LOCUS       DASWNS010000001         5287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_129760, whole genome shotgun sequence.
ACCESSION   DASWNS010000001 DASWNS010000000
VERSION     DASWNS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129760"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /gene="hemL"
                     /locus_tag="VGB76_00005"
     CDS             complement(<1..1216)
                     /gene="hemL"
                     /locus_tag="VGB76_00005"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY0097305.1"
                     /translation="MSEPITEIEQLTGEWLVAMVERDAARRLVIEPRKNLACLRRPPV
                     RRTFRRIRIDPMKPMSSEREDKSAELFARAVELMPGGVNSPVRAFRGVGGTPRFISRA
                     RGATMTDADGRTYIDYVGSWGPMILGHADPEVISALTDALTRGTSYGAPTELEIEMAE
                     EIIAAVPSIEMVRMVNSGTEATMSAIRLARGVTGRTKLLKFEGCYHGHGDSMLVRAGS
                     GVATLGLPDSPGVPPALAQETLTVAFNDPGALAEVFDAHGASIAAVIIEPVVGNMGCV
                     APHEGYLQTVREITSRHGALLIFDEVMTGFRLARGGAQELYGIAPDITTLGKIIGGGL
                     PVGAYGASRDIMRHVAPAGAVYQAGTLSGNPLAMTAGLTTLRRLRDKSVYAQLERAGA
                     RLCAGLSDAARAA"
     gene            complement(1655..2089)
                     /locus_tag="VGB76_00010"
     CDS             complement(1655..2089)
                     /locus_tag="VGB76_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097306.1"
                     /translation="MKKLLLLSVMALLLGGASEASAQWRDRDNRSDRQRIRRGVRSGQ
                     ITRDEARQIRERERQLRAERRAYRSDGVLTREERREIRRDEREQDRYIRSQRRDDDRR
                     NRRWDDDDNRRRGNGYWRRGAGSSSHPVFGNGGNRGRGRGRH"
     gene            complement(2364..3548)
                     /locus_tag="VGB76_00015"
     CDS             complement(2364..3548)
                     /locus_tag="VGB76_00015"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEY0097307.1"
                     /translation="MSTNFVEPLEQLATELLDNLADALVGKASTLRDGTEHAGVELPR
                     DLYAHAQAQTEWWYYTGHMRTASGRRFGFELVFFKRRTDLDRFGVVPLRLIANPLYLA
                     HFAVTDESRARFRYEHRKSANNPFDAPATMSANQYYLKLGDWTVREADGRHLLRATLG
                     DDLIFEVALRPTKPAALNGHAGVGVSFKDEGEASRYFSFTRMEAEGDITWGGETERFT
                     GAAWMDREFGTWRTTSKQKGWDWFSLQLSTGAELMVYQIRDAAGVPSEFSSGTFVDAG
                     GRATHLTRADFVLTTLAHWKSPHTGATYPIKWRLRAARFGIDVEIVPVLQDQELDTRG
                     TTMIVYWEGACKVRGRHNEAETEGRAYVELVGYDRSHEQPSFTTFLFGGALDRRWRSI
                     FG"
     gene            complement(3674..5188)
                     /locus_tag="VGB76_00020"
     CDS             complement(3674..5188)
                     /locus_tag="VGB76_00020"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEY0097308.1"
                     /translation="MNDKLIASIVAELAPALAGRMLGKVWQLSRVSLVIDFRLSDGRY
                     LFISIDPAAPRLYAIARTVRELEKLSLAPSNFVQTLRKHLGGARLVSLDKDEGERIVR
                     FAFASYDAVGEAHDWTLIAQLTGRAANLLLLDAAGRIADALRTPRGEGQEIADIYQPP
                     PQSSNSAGRPAPAPPFAQGAHASLSAAADAYYRELERARAFDARASAERARLRALVEK
                     QTKLRRNLERDLESHGDAAEHKRIGDLLLANIANAERRGSRVRVTDYFAEDAPVVELE
                     IDEHASLQEEAARRFTRYTKAKRAAQETTARLAELTHELDALARRQAELEEIIAARDE
                     AALESFGDAAARGAKGTRAKGAGRGGAPSSPGDAQRTKAQIKAAGEVAGARRYRSSDN
                     YEILVGRAAKDNDQLTFKVARAHDLWLHAADYPGSHVIVRNPARGADVPHRTIIEAAQ
                     LAAYFSQAKRDAKVSVHYTPRKFLSKPKGAAPGLVRMSSFRSILVEPREGVERI"
BASE COUNT          912 a         1651 c         1723 g         1001 t
ORIGIN      
        1 cggctgcgcg tgcggcatcg gaaagcccgg cacagagacg cgcgcccgcg cgctccagtt
       61 gcgcgtagac ggatttgtcg cgcaggcggc ggagcgtcgt gagaccggcc gtcatggcga
      121 gcgggttgcc ggagagcgtg cccgcctgat agacggcacc tgcgggcgcg acgtggcgca
      181 tgatgtcgcg gctcgcgccg taagccccga cgggcagccc gccgccgatg atcttgccga
      241 gcgtcgtgat gtcgggcgcg atgccgtaca actcttgcgc gccgccgcgt gcgagacgaa
      301 agccggtcat cacttcgtcg aagatgagca gcgcgccgtg acgactcgtg atctcgcgca
      361 ccgtttgcag gtagccttca tgcggtgcga cgcagcccat gttgccgacg acgggttcga
      421 tgatcactgc ggcgatgctc gcgccgtgag cgtcgaagac ttcggcgagc gcgccgggat
      481 cgttgaaggc gactgtgagc gtttcctgcg cgagcgcggg cgggacgccg gggctgtcgg
      541 gcagtccgag cgtggcgacg ccggagcctg cgcgcacgag catcgaatcg ccgtggccgt
      601 gatagcagcc ttcaaacttc aagagcttcg tgcgacccgt gacgccgcgc gccagacgga
      661 tcgcagacat cgtggcctcc gtgccgctat tgaccatgcg caccatctcg attgacggca
      721 cggcagcgat gatctcttcc gccatctcga tctcaagttc ggtgggcgcg ccgtaacttg
      781 tgccgcgtgt gagcgcgtcc gtcagggcgg agatgacttc cgggtcggcg tggccgagga
      841 tcatcggacc ccatgagccg acatagtcaa tgtaggtgcg cccgtctgcg tcggtcatcg
      901 tcgcgccgcg cgctcggctg atgaagcggg gcgtgccgcc gacgccgcga aaagcgcgca
      961 caggggagtt gacgccgccc ggcatcaact cgacggcgcg tgcgaacaat tccgcagatt
     1021 tatcttctct ctcgctgctc ataggtttca tcggatcaat ccttattcgt ctaaaagttc
     1081 ggcgcaccgg aggtcttcga aggcaagcca gattttttcg aggttcgatc accagacgcc
     1141 gggctgcatc ccgctcgacc attgccacaa gccactcgcc cgtgagttgt tcgatctccg
     1201 tgattggctc agacatgtgc aggcatcttt ggctaaccgt cttccgtgcg tctggtaaag
     1261 cctgtgttgg aacagtttcg ataatagccg attcaggcgg agcggcgcat tgcccgcgag
     1321 agttcgcacg gtgcgcggag agcgtctccc ggcgcgaggg cgtggtcccg ccgacgacgc
     1381 gcgccttcgc acgcaagctc accgaactcg gcgtcgaaca tatttatcga ggagtacaac
     1441 ggcgaccacc gcaatcgcat gtggggacgc agcggcagac tctacacgga agtcctgccc
     1501 tacttctggc tcctgctcga tcccgcagaa cgctaaaccg gagaggctga atgagcgaaa
     1561 cccgcgcaga cgttttaacg tccgcgcggg ctgcgccgtc gggaaactct taagtctgac
     1621 tattaagact cgaactcgat ttcagttcag tcgcttagtg acgaccgcga ccacgtccac
     1681 ggttgccgcc gttgccgaac acgggatgcg aagatgaacc cgctccgcgt cgccagtaac
     1741 cgttgccgcg cctgcggttg tcgtcatcgt cccaacgccg gttgcgacgg tcatcgtcgc
     1801 ggcgttggct gcggatgtag cgatcctgtt cgcgctcgtc gcggcgaatc tcgcgccgct
     1861 cttcacgcgt cagcacgcca tcggaacggt aagcgcgccg ttcggcgcga agctgccgtt
     1921 cgcgttcacg tatctgacgt gcttcgtcgc gcgtgatctg gccggagcgg acgccgcgcc
     1981 ggatgcgttg acggtcggag cggttgtcgc ggtcgcgcca ctgcgcggac gcctcgctcg
     2041 cgccgcccag aagcaaggcc ataacgctca gaagtagtaa ctttttcatc gttcaaccct
     2101 ttcctgatat tcgatactgt gaaaattacg gcatttcaat taacggctgc ctgcgcaccg
     2161 tcggagagag tttaagcaag cgtacacgat aaagggcggc aatcatcatg ccacgcaccg
     2221 gatgcgcacg cgatctcagt ggccggaggc ttaaactgtt gataactcaa gctgcgcccc
     2281 gatcacgcct gcgttgcgct ctctccgcgt tgcgtcacca aatagaacag cgggtgaatg
     2341 atttacacca tctcgtgccg tttctagccg aagatactgc gccagcggcg gtcgagcgcg
     2401 ccgccgaaga ggaatgtggt gaaggacggt tgctcgtgcg aacggtcgta gccgacgagt
     2461 tcgacgtaag cgcgcccctc agtctcggcc tcgttgtgcc gtccgcgaac cttgcacgcg
     2521 ccctcccagt agacgatcat cgtcgtcccg cgcgtgtcga gttcctgatc ctgcaacacc
     2581 ggaacaatct ccacgtcgat accgaatcgc gccgccctga gacgccactt gatcgggtag
     2641 gtcgcgcccg tgtgcgggct tttccaatga gccagcgtgg tgagcacgaa gtctgcgcgc
     2701 gtgaggtgcg tcgcacgacc cccggcgtcc acaaacgttc cgctggaaaa ctccgagggc
     2761 acgcccgccg cgtcgcgtat ttgatagacc atcaactccg cgcccgtcga gagttggagg
     2821 ctgaaccagt cccagccctt ctgcttgctc gtcgtgcgcc aggtgccgaa ctccctgtcc
     2881 atccacgccg cgcccgtgaa gcgttccgtc tcgccgcccc aggtgatgtc gccctccgcc
     2941 tccatccgcg tgaaggaaaa atagcgcgac gcctcgcctt catctttgaa actgacgccg
     3001 acgcccgcat gcccgttgag cgcggcgggt ttcgtggggc gcaaagcgac ttcgaagatg
     3061 agatcgtcgc cgagcgtggc gcgcaggagg tgccgcccgt cagcctcgcg cacagtccag
     3121 tcgccgagct tcaaataata ttggttcgcg ctcatcgtcg cgggcgcatc gaaagggttg
     3181 ttcgcgctct tgcggtgctc gtagcgaaag cgcgcgcgcg actcgtcagt gacggcgaag
     3241 tgtgcgagat agagcgggtt ggcgatgaga cgcaggggga cgacaccgaa gcggtcgagg
     3301 tctgtgcgac gtttgaagaa gacgagttcg aaaccgaagc gacgaccgga ggccgtgcgc
     3361 atgtgtccgg tgtagtacca ccattcggtc tgcgcttgcg cgtgcgcgta gagatcgcgc
     3421 ggcagttcga cgcccgcgtg ctccgtgccg tcgcgcagcg tcgaagcctt gccgacgagc
     3481 gcgtccgcca gattgtcgag aagctcggtc gccagttgtt ccagaggttc gacgaagttt
     3541 gtgctcatgc agctcgattg ctccaatgcg tgttctgaaa attcccgaac acgataaccg
     3601 aagggcgtaa cgaaactgtg cgcggatgct tacaaaagag aggatgcgcc ggaacggttc
     3661 gtcaaatgcg ttgtcaaatg cgttcgacgc cttcgcgcgg ttcgacgagg atgctgcgga
     3721 aactcgacat gcggacgagg ccgggggctg cgcccttcgg cttggagaga aacttgcgcg
     3781 gcgtgtaatg gacggagact ttggcgtcgc gtttggcctg gctgaaatag gctgcgagtt
     3841 gcgccgcctc gatgatggtg cgatgcggca cgtccgcgcc gcgcgcggga ttacgcacga
     3901 tgacatgcga gccgggatag tctgcggcat gcaaccagag atcgtgcgcg cgtgcgacct
     3961 tgaaggtgag ttgatcgtta tccttcgctg cgcgcccgac gagaatttcg tagttgtccg
     4021 acgaacgata gcgacgcgcg cccgccacct ccccggcagc tttgatttga gccttcgtgc
     4081 gctgcgcgtc tccgggtgac gatggcgcgc cgccgcgccc cgcgcccttc gcgcgagttc
     4141 ctttcgcgcc gcgagccgcc gcgtcgccaa acgattcgag cgcggcctcg tcgcgtgcgg
     4201 cgatgatctc ttcgagttcg gcctgtcgcc gtgcgagcgc gtcgagttcg tgtgtgagtt
     4261 cggcgaggcg cgcggtcgtc tcctgcgcgg cgcgcttggc cttcgtgtag cgcgtgaagc
     4321 ggcgcgcggc ctcttcctga aggctcgcgt gttcgtcaat ctcaagttcg acgaccggag
     4381 catcctccgc aaaatagtcc gtcacgcgca cgcgcgaacc gcgacgctcg gcgttggcga
     4441 tgttggcgag cagaaggtcg ccgatgcgct tgtgctcggc ggcgtcgccg tgcgactcca
     4501 gatcgcgttc cagattgcga cgcagtttgg tctgcttctc gacgagcgcg cgaagccgcg
     4561 cacgctcggc ggaggcgcgt gcatcgaagg ctctcgccct ttcgagttcg cggtaataag
     4621 catcggcagc ggcggagagg gaagcgtgcg cgccctgcgc gaaagggggc gcgggagccg
     4681 gtctgcccgc gctattacta ctctgcggcg gcggttgata gatgtctgcg atctcctgtc
     4741 cctcgccgcg cggcgtgcgc agtgcatcgg cgatgcgacc cgccgcgtcg agcagcaaca
     4801 ggttggcggc gcgtcccgta agttgcgcaa tgagcgtcca atcatgcgcc tcgcccaccg
     4861 cgtcgtagga ggcgaaggca aagcgcacga tgcgttcgcc ctcgtccttg tcgagactga
     4921 cgagccgcgc gccgcccaga tgtttgcgca aggtctgcac gaaattcgac ggcgcgagcg
     4981 acagtttttc gagttcgcgc acggtgcgcg cgatagcgta gagacgcggc gcagccgggt
     5041 caatggagat gaacaggtag cggccatcac tcaaacgaaa atcgatgacg agcgagacgc
     5101 gcgacagttg ccacaccttg cccaacatgc gccccgcgag cgcgggcgcg agttcggcga
     5161 cgatgctggc gatcaatttg tcgttcatcg agaagctggc ggtgtccggt tgtccgtgtt
     5221 tagttgtccg tcgtcagttg ctagtcgtcc gccgttcatc tctcatgcgt aacgcggcgg
     5281 agacggc
//
LOCUS       DASWNS010000002         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_363346, whole genome shotgun sequence.
ACCESSION   DASWNS010000002 DASWNS010000000
VERSION     DASWNS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363346"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VGB76_00025"
     CDS             <1..587
                     /locus_tag="VGB76_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0097309.1"
                     /translation="RRAVFIDRDGTISEEVGYVNHPSRYRVFPYAAEAIKRLNESNWL
                     AILVTNQAGVARGYFTEDVIGAVHGLLAEELARGGAHLDAVYYCPHHPSVGEPPYRFD
                     CDCRKPRPGLIRRAAADFDIDLAGSWMVGDRYSDTELARNAGTRAALVLSGYGRGEWE
                     YQRAAWRHQPDLVAENLLEGVEKIISDGGTGSTD"
     gene            642..>1618
                     /locus_tag="VGB76_00030"
     CDS             642..>1618
                     /locus_tag="VGB76_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0097310.1"
                     /translation="MLQTRLIHLVHQFAERRVVVVGDLLADQFLYGEISRVSREAPVL
                     ILRHERTETVPGGAANCAVNLASLGAQVSLVGTVGADTAGDALLERLRAAGVDCGGVV
                     RAPELSTTTKVRILAGQAHSMRQQVIRVDYEGQPLSDARIKTELTGRLNEATSRADAI
                     IVSDYNYGVACAEMIDTLGAAMQRHELPVIVDSRFRLLDFKGFTSATPNEDEVEQVLG
                     RRLEDASALEAAGAELCARLGYKALLITRGSQGMLLVEPGARPLHLSPAGSREAVDVT
                     GAGDTVIATYTLAVASGGGFMYATYLANHAGGVVVMKRGTASLDTRELLA"
BASE COUNT          279 a          497 c          549 g          293 t
ORIGIN      
        1 aacgtcgcgc cgtcttcata gatcgcgacg gcacgatctc ggaagaggtc ggctacgtca
       61 atcatccttc gcgctatcga gtctttcctt acgcggcgga agcgatcaag cgtctcaacg
      121 aatcgaactg gctcgccatc ctcgtcacga atcaggcggg cgtcgcgcgc ggctacttca
      181 ccgaagacgt gatcggcgcg gtgcacggcc tgctcgcgga agaactcgcg cgcggcggcg
      241 cgcatctgga cgcggtctat tattgcccgc atcatccgtc ggtcggcgag ccgccgtatc
      301 gtttcgactg cgactgccgc aagccgcgcc ccggactgat ccgccgcgcc gccgccgatt
      361 tcgatattga cctcgcgggc agttggatgg tcggcgaccg ttacagcgac actgaactcg
      421 cgcgcaacgc aggcacgcgc gccgccctcg tcctcagcgg ctacgggcgc ggcgagtggg
      481 aatatcaacg cgcggcatgg cgacaccagc ccgatctcgt cgcggaaaat ctgctggaag
      541 gggtagagaa aattatttcg gacggcggaa ccgggagtac ggattaacag gctccttcga
      601 gcggttttcc gccgaacgtt ttcgccattc gtagttacga tatgcttcaa acgcgtttga
      661 tacatctggt tcaccagttc gcggagcgac gcgtcgtagt ggtgggcgat ctgctggccg
      721 atcagttttt gtacggcgag atcagccgcg tgtcgcgcga agcgcccgtc ctgattttgc
      781 gccacgaacg gacggaaacc gtgccgggcg gcgcggctaa ctgcgccgtc aatctggcct
      841 cgctcggcgc gcaggtttcg ctcgtcggca cggtcggcgc ggacacggcg ggcgatgcgc
      901 tgctcgaacg cttgcgcgcg gcgggagttg attgcggcgg ggtcgtgcgt gctcccgaac
      961 tgagcacgac gacgaaggtg cgcatacttg ccggtcaggc gcactcgatg cggcagcagg
     1021 tgatccgcgt ggactacgaa ggccagccgc tctcggatgc gcggatcaag acggaactca
     1081 ccgggcgtct gaacgaagcg acgtcgcggg cggatgcgat catcgtctcg gactacaact
     1141 acggcgtcgc atgcgcggag atgatcgaca ccttgggcgc ggcgatgcag aggcacgaac
     1201 tgcccgtgat cgtggattcg cgtttccggc tcctcgattt caagggtttc acttcggcga
     1261 cgccgaacga ggacgaggtg gaacaggtgc tcggtcgaag gctcgaagac gcttcggcgt
     1321 tggaggcggc gggcgcagag ttgtgcgcac ggctcggtta caaggcgttg ctcattacgc
     1381 gcggcagcca ggggatgctg ctcgtcgaac ccggagcgag gccgctgcat ctctccccgg
     1441 caggctcgcg cgaagcggtg gacgtgacgg gcgcgggcga cacggtgatc gccacctaca
     1501 cgctcgcggt cgcttccggc ggggggttta tgtatgccac ctatctcgcc aatcacgccg
     1561 ggggcgtcgt cgtgatgaaa cgcggcacag cctcgctcga tacgcgggaa cttctggc
//
LOCUS       DASWNS010000003         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_78050, whole genome shotgun sequence.
ACCESSION   DASWNS010000003 DASWNS010000000
VERSION     DASWNS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78050"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1983)
                     /locus_tag="VGB76_00035"
     CDS             complement(52..1983)
                     /locus_tag="VGB76_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HEY0097311.1"
                     /translation="MTSILGLSAFYHDSAACLVVDGEIVAAAQEERFTRRKHDHRFPT
                     HAVRYCLAEAGLGVDALDYVSFYDKPLLKFERLLETYLDYAPKGFPSFLKAMPAWMKE
                     KLWMSDLISTEMARVEGVEDAKEAKRRAKSFEWKLLFGDHHESHAASAFYPSPFEEAA
                     ILTLDGVGEWATSSIGVGRGSELSLLKELRFPDSLGLLYSAFTYYTGFRVNSGEYKVM
                     GLAPYGEPKYVALIKERLVEIRDDGSLRMNHEYFGYSQGLKMTSKAFSKLFGDEPRKP
                     EAKLTQREMDLARSIQVITEEVMLKMATYAHRETGARKLCMAGGVALNCVGNGRILRE
                     SPFEDIWIQPAAGDAGSAAGIALAIWHRYLGQPRTSPERAGTWRSPRSANGANGSLPP
                     YADGMKGSYLGPRHTEAEIEEFLRARNLPYKKYRREELPEAVAELLAHEKVIGLHQGR
                     MEFGPRALGGRSIIGDARSPRMQSVMNLKIKYRESFRPFAPSVTREQVSEWFDLNTDS
                     PYMLLVAEVVQRRRREATEAERALWGIEKLNAVRSDIPAVTHVDYSARIQTVRRETNP
                     LYWEIIEAFQNKTGCPVVVNTSFNVRGEPIVCTPEDSYRCFMRTEMDALVLETCVLLK
                     EEQPEFAEAADWRESFALD"
     gene            complement(2007..2165)
                     /locus_tag="VGB76_00040"
     CDS             complement(2007..2165)
                     /locus_tag="VGB76_00040"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEY0097312.1"
                     /translation="MSKSQVLLQLWELMKYNKKYWLLPIILVLVLTGLALVFAQSSAL
                     APFLYPLF"
BASE COUNT          433 a          730 c          675 g          470 t
ORIGIN      
        1 cggcgcgtta aaaaatcccc tgcgcgagcg aaagcgtccg cgccgccggt tctaatccag
       61 cgcaaaactc tcgcgccagt ctgcggcttc ggcaaactcc ggctgctcct ctttcaagag
      121 cacgcaggtt tcgaggacga gcgcgtccat ctccgtgcgc atgaagcagc gataactgtc
      181 ctcgggcgtg cagacgattg gctcgccacg cacgttgaaa ctcgtgttga cgacgacggg
      241 gcagccggtc ttgttttgaa aggcttcgat gatctcccag tagagcggat tggtttcgcg
      301 ccgcacggtc tggatgcgcg ccgaataatc cacgtgcgtg acggcgggaa tgtcggagcg
      361 cacggcgttc agtttttcga tgccccagag agcgcgttcc gcttccgtcg cctcacggcg
      421 gcgacgttga accacctctg cgacgagcag catgtaaggg ctgtccgtgt tcaggtcaaa
      481 ccactcggac acctgttcgc gcgtcacgct cggcgcaaac ggacgaaagc tctcgcggta
      541 tttgatcttc agattcatca ccgactgcat gcggggcgaa cgcgcgtcgc cgatgatgct
      601 gcgtccgccg agggcgcggg gtccaaactc catgcgaccc tgatgcaggc cgatcacttt
      661 ttcgtgcgcc agcaattccg cgacggcttc cggcagctcc tcgcggcggt attttttgta
      721 aggcagattg cgcgcacgca aaaactcttc gatctccgcc tccgtgtggc gtggtcctaa
      781 atacgaacct ttcatgccgt cggcataagg cgggagcgag ccgttcgccc cgttcgcact
      841 gcgcggcgaa cgccacgtcc ccgcgcgttc gggactcgtg cgcggctggc ctagatagcg
      901 gtgccagatg gcgagcgcga ttccagccgc gctgcccgca tcgcccgccg ccggttgaat
      961 ccagatgtct tcaaacggac tctcgcgcag aattctcccg ttgccgacgc agttcagagc
     1021 gacgccgccc gccatgcaaa gtttccgcgc gcccgtctct cgatgcgcgt aagtcgccat
     1081 cttcaacatc acttcttcgg tgatgacctg aatgctgcgc gcgaggtcca tctcgcgctg
     1141 cgtcagtttc gcttccggct tgcgcggctc gtcgccgaac aacttcgaga aggctttgct
     1201 cgtcatcttc aagccctgcg aatagccgaa gtattcgtgg ttcatgcgca ggctgccgtc
     1261 gtcgcgtatc tctacgagtc gctccttgat gagcgcgaca tacttcggtt cgccgtaagg
     1321 cgcgagtccc atcactttgt attcgccgct gttgacgcga aagcccgtgt agtaagtgaa
     1381 cgcgctgtag agcagtccga gactgtcggg gaagcgcaac tccttcaata aactcaactc
     1441 gctcccgcgc ccgacgccga tggaactcgt cgcccattcg cccactccgt ccagagtcag
     1501 gatggcggcc tcttcaaacg gcgaagggta aaacgcgctc gccgcgtggc tctcgtgatg
     1561 atctccgaac agcagtttcc actcgaaact tttcgcgcgc cgcttcgcct ccttcgcgtc
     1621 ttcgacgcct tcgacgcgcg ccatctccgt cgagatcaaa tccgacatcc acagtttctc
     1681 cttcatccag gcgggcatcg ctttgaggaa ggagggaaag cccttcggcg cgtagtcgag
     1741 ataagtttcg aggaggcgtt cgaatttcag gagcggcttg tcgtagaaag agacgtagtc
     1801 gagcgcgtca acgccgagtc cggcttcggc gaggcaatag cggacggcgt gtgtggggaa
     1861 acggtggtcg tgcttgcggc gcgtgaagcg ttcctcctgc gccgccgcga cgatctcgcc
     1921 gtccacgacg aggcacgccg cagagtcgtg atagaaagcc gatagtccga gaatactggt
     1981 cacaaaaatt cacccgcgcc ttcgcgtcag aacagtggat agaggaacgg ggcgagcgcg
     2041 gagctttgcg cgaagacgag tgccagcccc gtcagcacga gcacgaggat gatcggcaag
     2101 agccagtact tcttgttgta tttcatcaac tcccacaact ggagcagaac ctgcgatttc
     2161 gacatctaac aaccgccttc cctctaatac atcttctcgt agtgcttggg gtctttgcgc
     2221 cgctctgtgt agggcttcca gtaagactcc gtgcgacccc cgcgccgacc gagcgggtcg
     2281 ccgcccgaca gacggcgcag gaatccga
//
LOCUS       DASWNS010000004         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_200, whole genome shotgun sequence.
ACCESSION   DASWNS010000004 DASWNS010000000
VERSION     DASWNS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VGB76_00045"
     CDS             complement(<1..750)
                     /locus_tag="VGB76_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0097313.1"
                     /translation="MLEHFHILLARLRALVRRDVVIEEIDEELRLHIELETEANIERG
                     MPPGEARRAALRSFGNLGRIKDAAYEIRGGGMLEALIQDIRYGARMLVKHRAFTFIAV
                     LTLALGIGANTTIFSVVNAVLLRPLPYPEPERLVQVSWLLGDGSGESLSTAVYAFWKD
                     NMRSFEEAAGYAQTNAGFNLAGGAEPRRVRGLRVAEGFLRVLGVNPMLGRSFTAEEDR
                     PNAPCAAVISEGLWQGYFGGDASTVGRQVELN"
     gene            complement(761..1093)
                     /locus_tag="VGB76_00050"
     CDS             complement(761..1093)
                     /locus_tag="VGB76_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0097314.1"
                     /translation="MTKATADLLQGTLDMLILKALSLGALHGYGIIQRIRQMSDEMLT
                     VEQGSLYPALYRIEQRGWVSSTWDTNETGRRAKFYALTRTGRKQLEVEEASWDRLVLA
                     VTKMRQAT"
     gene            complement(1346..1651)
                     /locus_tag="VGB76_00055"
     CDS             complement(1346..1651)
                     /locus_tag="VGB76_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HEY0097315.1"
                     /translation="MLKTPIGRLRVIAFIEGISYLLLLGVAMPLKYFAGMPAPVKTAG
                     WIHGVLFMLYLFSVAEVTIRRRLSFMLAFGALVASLIPFGTFVLDARLRREERAERS"
     gene            1912..2241
                     /locus_tag="VGB76_00060"
     CDS             1912..2241
                     /locus_tag="VGB76_00060"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0097316.1"
                     /translation="MGNERRLQERVNIVLEARWEGLAGRHEARISNLSSGGCFIESIG
                     QVQLHEQVRFEIQLPWGRWLPLQGEVVYYQPNFGFAIHLTELSEMQQRQFNALFAYAK
                     ASTHKSA"
BASE COUNT          532 a          720 c          666 g          471 t
ORIGIN      
        1 gttcaactcg acctgcctgc cgactgtcga agcatcgccg ccgaagtagc cctgccacag
       61 cccttcacta atcaccgccg cgcagggagc gttcgggcga tcctcctccg ctgtgaaact
      121 ccgacccagc atcgggttga cgccgagtac gcgcaggaaa ccctccgcga cacgcaagcc
      181 ccgcacgcgt cgcggctccg cgccgcccgc aagattgaaa ccggcattcg tctgggcgta
      241 gcccgccgcc tcctcgaacg aacgcatgtt gtctttccag aaagcgtaga ccgcagtgct
      301 cagcgactcg cccgacccgt cacccagcaa ccatgaaacc tgcaccagcc gctcaggctc
      361 cgggtaaggc agcggtcgca gcaggacggc attgacgacc gagaagatcg tcgtgttggc
      421 gccgatgccg agcgccagcg tgagcacggc gataaaggtg aaggcacgat gcttgacgag
      481 catccgcgcc ccgtacctga tgtcttgaat gagtgcttcc aacatgcccc ctccacgaat
      541 ctcgtaagct gcgtctttaa tccggcctag attgccgaag ctcctgagcg ccgcccgcct
      601 cgcctctccc ggcggcatgc ctctctcgat attcgcctcc gtctccaact cgatgtgaag
      661 gcgtagctct tcgtcgatct cttcgatcac cacgtcgcgt cgaaccagcg cgcgcagacg
      721 tgccagcagg atgtgaaagt gttccaacat cgcccgctcc ttatgtagcc tgacgcattt
      781 tcgtaacggc cagcacgagc cggtcccaac tcgcctcttc gacttcaagc tgcttccggc
      841 ctgtcctcgt aagcgcgtaa aacttggcgc gccgccccgt ctcgttcgtg tcccaggtgg
      901 aactgaccca cccgcgctgc tcgatgcggt agagcgccgg gtagagcgaa ccctgttcca
      961 ccgtcaacat ttcgtcggac atctgccgga tacgctggat gatgccgtac ccgtgcagcg
     1021 cacccagcga aagcgccttg aggatgagca tgtcgagtgt gccttgaagc aggtctgcgg
     1081 ttgctttggt catcgctata tctccaattt ggtagcccgc gacagagcgt gatacctaga
     1141 atgggaaggc attctaaata aggacgccta gaacgtcaag gcttaaatga aaactgtgtg
     1201 atgaattcca gaaacgggga ggcaagccgt cagggtagtg ttgaaaaaat tccccggtag
     1261 gatatgctct tgacaggctc tagccgcgtg gcgcgggcat cagggtcttc ccttcgtcgg
     1321 gagcactatt ttatcttctg acacgttaac ttcgctccgc gcgctcctcc cggcggagac
     1381 gcgcatcgag cacgaaagtg ccgaagggga ttaacgacgc caccagcgcg ccgaaggcca
     1441 gcatgaaaga taagcgtcgc ctgatggtta cctcggcgac ggagaacagg tagagcatga
     1501 acagtacgcc gtgaatccaa ccggcagttt tgacgggggc aggcataccg gcgaagtact
     1561 tcaggggcat cgccactccg agcagcagga ggtacgagat gccctcgatg aaagcgatga
     1621 cgcgcaggcg accgatgggg gttttcagca tgtctcaact ccaacaaata aacgtcagtt
     1681 gaaaatcaca gtatcacggt tacttaatca taccggcagt ggggcgaaaa ctttccggcc
     1741 acgccggttc ctgaggtctt tgcgctctac ccgtctctga taattgtggc taaaatattt
     1801 tttgcaacgt tacctgtgaa gctactcgca gccgccgcta actgcaaagg atatcaggcg
     1861 ggtaaagaga agcgatgatg taaacgaacg agagacgctc gaccggaaaa gatgggcaac
     1921 gaacgcaggt tacaagagcg ggtcaatatt gttctcgaag ccagatggga agggctggcg
     1981 ggcaggcacg aggcgcgcat ctccaacctg agttccggtg ggtgcttcat cgaatcaatc
     2041 gggcaggtgc aacttcacga gcaggtgcga ttcgagattc aattgccgtg ggggcgttgg
     2101 ctgccgctgc aaggcgaagt cgtctactat cagcccaact tcggtttcgc tatccacttg
     2161 acggagttat ccgagatgca gcagcgccag ttcaacgcgc tgtttgccta cgcgaaagcg
     2221 tcaacgcaca agtcagcgta aacgcaccca acgacatcat ccccgctcgc tcttgcacga
     2281 ttagtctgat atagcggctt aaacatttgt ggcaactctg cacgcattat tcaccacgtc
     2341 tgatgaggct ggaggcaacg gccaccagtt cgaccagttc cacaggctt
//
LOCUS       DASWNS010000005         6428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_311562, whole genome shotgun sequence.
ACCESSION   DASWNS010000005 DASWNS010000000
VERSION     DASWNS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311562"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..2295)
                     /locus_tag="VGB76_00065"
     CDS             complement(202..2295)
                     /locus_tag="VGB76_00065"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0097317.1"
                     /translation="MSEHKHTNRLQHETSPYLLQHAHNPVDWYPWGDEALGRARAENK
                     PILLSIGYSACHWCHVMERESFENEEIASLMNENFINIKVDREERPDLDQIYMNAVQL
                     MLGHGGWPLTVFLTPELVPFYGGTYFPPEDRFRLPGFPRVLSGVADAYRSQPDEIAQT
                     TTSFLNELRRMSVAYESNEPLDEQLLDAAASVLTRNYDPQHGGFGGAPKFPASMNLEF
                     LLRVNQRRNGDGQILKMVTETCRRMAEGGLYDQLGGGFHRYSTDAQWLVPHFEKMLYD
                     NALLSRLYLHVYQVTGDQFFRRITEETLDYVVREMTDADGAFYSTQDADSEGVEGKFF
                     VWSIEEIGELLGEEDARVVSAYYGATHAGNFEERNILHVPRPAQSVAAELNISVERLH
                     EVVERSKPLLFAAREKRIKPGRDEKAIAAWNGMMLTSFAEASSVLERDDYRRIAERNA
                     GFILDTLRREGTLQHVYKDGQAKHHAYLDDYACVASALLSLYEATGAIRWLRECISLT
                     GQMCEEFWDETDGGFFYTSTRGERLIVRNKDFMDNATPSGNSVAAELLLRLALLTENQ
                     EHQRKATTIFRLLRDQTVRHPSAFGYLLGALDLYLSTPREIVIVGEVGAHDSLALLRE
                     VWRRYLPNKVVAQSAENDAEAAGILPLLRERPALGGRATAYVCENYSCLQPVTTPGEL
                     AAQLDTARTKGAPGN"
     gene            2469..4181
                     /locus_tag="VGB76_00070"
     CDS             2469..4181
                     /locus_tag="VGB76_00070"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY0097318.1"
                     /translation="MERESLEMDVVFVGAGPANLSGALHLARLVKEHNEGVAAGKREG
                     KPLGELEIGVIEKGAAVGSHILSGAVMDPKALRELIPDFIEQGAPVESPVKEDEFLYL
                     TETRAIRSPITPPPLKNHGYYIVSLNKLVAWLGEQCEAAGVNIFPEFPGAEMLYDGED
                     RVVGVRTGDKGIDKEGKRKANFEPGVDLLAKVTVLGEGPRGSLTKSLAQRLNLNDGRE
                     PQVYSLGIKELWELPDDRYPAGRVTHTLGYPSDAHTYGGGWIYGMQNRILNLGYVTGL
                     DYRDPLLDPHAEFQRFKQHPFLAKLLEGGKLLRYGAKTIAAGGYYAMPQLYADGVLLV
                     GDCAGFLNSQRLKGIHTAIKSGMLAAEAIFEALVAGDFSARQLQRYETLVKESWIMPE
                     LRKVRNFHAGFRRGRWLGIANAGLQFLTGGRSWGVFDRAEAESGHAALKKLSDFGYKG
                     DALEQRYKGLRFDGKLTFNKLTDVYHAAVGHDEDQPVHLHVLDTNICVERCAEEYGNP
                     CQRFCPAAVYEMVEDPATAGKRRLQINFSNCVHCKTCDIMDPYQIINWVTPEGGGGPE
                     YKNM"
     gene            4274..4726
                     /locus_tag="VGB76_00075"
     CDS             4274..4726
                     /locus_tag="VGB76_00075"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY0097319.1"
                     /translation="MRLAEESQARLEQFFRWYEGDEQLRLPLIFIHAGFLSHRMARVL
                     RSAAFTVGRHIFVSQVYVERDERGQLTIRGGLLAHEATHVRQFQREGFLPFLYNYLRE
                     YVTFLFRTGKLNAMARRAAYEQITREREAREVEAAYLEWRLNATSSPG"
     gene            complement(4754..4996)
                     /gene="rpoZ"
                     /locus_tag="VGB76_00080"
     CDS             complement(4754..4996)
                     /gene="rpoZ"
                     /locus_tag="VGB76_00080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY0097320.1"
                     /translation="MAKATQSNKEDHNTPATAEKQTPAMDSKYRMIIVAAQRSKQLQR
                     GARARVEIDSQQHKHTRIALLEVEQGKVDFTITEQE"
     gene            complement(5100..5744)
                     /gene="gmk"
                     /locus_tag="VGB76_00085"
     CDS             complement(5100..5744)
                     /gene="gmk"
                     /locus_tag="VGB76_00085"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY0097321.1"
                     /translation="MDANESPALDQPERGILFVISSPSGGGKGTLIRRILTHLPRLGY
                     SVSWTTRAPRQGETNGRDYHFVTTEEFDAARERGEFLEWALVHGNLYGTSLKVVEREL
                     AAGRDIVLEIDVQGAATVRRTGIEAVSIFILPPSFEILRRRLIGRQSENSEALAVRLQ
                     NSRGEVEDYREFDYLVLNDDVGRAATQLAGIVYAERARRPRQERLAQRVLATFP"
     gene            complement(5755..>6428)
                     /locus_tag="VGB76_00090"
     CDS             complement(5755..>6428)
                     /locus_tag="VGB76_00090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY0097322.1"
                     /translation="EVAYEMNRPLIAGFIEAMRSVQQEFSLAGEPDINVLARLPGALQ
                     PVRDGSLTEAMREGIERAVMEALAELNHMRGVEGEALAAEMHARLDEIERQVPVIEAV
                     ASDLVETYRARLQRRISELLARDGLDIVELDQGRLAQEVAYLADRSDISEELARLRSH
                     VKQFREIIDSEGDTGKRLDFLLQEFNREANTVLSKSTDMAIKEAALAIKAEVEKLREQ
                     VQNVE"
BASE COUNT         1272 a         1878 c         1918 g         1360 t
ORIGIN      
        1 gagtccgggc cggattccat aaacgtcgtc gcaaccgact cttttgcgac tttgcgcctc
       61 cggcgtgagt ttaaattctc gctggaggcg caaggtcgca aagaaaactc ggagcgttgt
      121 gcggcgacga acagttttcc caataagctc gaagcaatat ttaggaaaga gcttaataga
      181 gagcttgtga ggaaaggcgc gctagttgcc gggcgcgcct ttcgttctgg ccgtgtcgag
      241 ttgcgcggcg agttcgccgg gcgtggtcac gggttggagg cagctatagt tttcgcagac
      301 atacgcggtg gcacgaccgc cgagcgcggg tcgttcgcgc agaagcggaa ggatgcccgc
      361 tgcctccgcg tcgttctccg ccgactgcgc gacgaccttg ttcggcaggt agcgtctcca
      421 gacttcacga agcaatgcga gcgagtcgtg cgctccgacc tcgccgacga tgacgatctc
      481 tctcggggtc gaaagataga gatcgagtgc gccgagtaga taaccgaagg cggaggggtg
      541 gcgcacggtt tgatcgcgca ggagacgaaa gatcgtggtg gctttgcgct ggtgttcctg
      601 attctccgtg aggagcgcga gacgcaggag cagttcggcg gccacggagt ttccggaggg
      661 cgtggcgttg tccatgaaat ctttgtttcg cacgatcaaa cgctcgccgc gcgtgctcgt
      721 gtagaagaag ccgccgtccg tctcgtccca aaactcctcg cacatctgtc cggttagaga
      781 gatgcactcg cgcaaccaac gaatcgcgcc ggtcgcttcg tagagcgaaa ggagggcgga
      841 ggcgacgcac gcataatcgt cgagataggc gtgatgcttg gcctgcccat ctttatagac
      901 gtgttggagc gtgccttcgc ggcgcagcgt gtcgagaatg aacccggcgt tgcgttcggc
      961 gatgcgacgg taatcgtcgc gttcgagcac ggacgaggct tcggcaaaac tcgtgagcat
     1021 catgccgttc caggcggcaa tcgctttctc gtcacgcccc ggtttgattc tcttttcccg
     1081 cgccgcgaag agcagaggct tgctgcgctc gacaacttca tgcagacgct ccacagagat
     1141 gttcaactct gcggcgaccg actgcgcggg gcgcggcacg tgcaaaatgt tccgctcttc
     1201 aaagtttccc gcgtgcgtcg cgccgtaata ggctgacacc acgcgtgcat cttcttcgcc
     1261 caacaactct ccgatctctt cgattgacca gacgaagaat tttccttcca cgccttccga
     1321 gtcggcatcc tgcgtggaat aaaacgcgcc gtccgcgtcc gtcatctcgc gtacgacgta
     1381 atcgagcgtc tcttctgtga tgcggcgaaa aaattgatcg cctgtcactt gatagacgtg
     1441 caggtagaga cgcgagagca gggcgttgtc gtagagcatc ttttcaaaat gcgggacgag
     1501 ccactgtgca tcagtcgagt agcgatgaaa gccgccgccg agttgatcat acaaaccgcc
     1561 ttcggccatc cggcggcacg tctccgtcac catcttgaga atttggccgt cgccgttacg
     1621 gcgttgatta acgcgcaaga ggaattcgag attcatggag gcggggaatt ttggcgcgcc
     1681 gccgaagccc ccgtgctgcg gatcgtagtt gcgcgtgagc acagacgccg ccgcgtcgag
     1741 cagttgctcg tcgagcggct cgttcgattc gtaggcgacg ctcatccggc gcagttcgtt
     1801 cagaaaactt gtcgtcgtct gcgcgatctc gtccggttgc gaacgatagg cgtcggccac
     1861 gccggacaag acgcgcggga agccgggcaa gcggaaacga tcttcgggcg ggaaatatgt
     1921 gccgccgtag aaaggcacga gttcgggcgt gaggaagacg gtcaaaggcc agccaccgtg
     1981 gccgagcatc aactgcaccg cattcatgta aatctggtcg aggtcggggc gttcttctcg
     2041 atcaactttg atgttgatga aattctcgtt catcaacgaa gcgatctctt cgttctcgaa
     2101 cgactcgcgc tccatcacgt gacaccagtg gcaggccgag tagccgatgc tgagtaagat
     2161 cggtttgttc tccgcgcgtg cgcgccccaa cgcttcatcg ccccacggat accagtcaac
     2221 cggattgtgc gcgtgctgca acagatatgg actcgtctcg tgttgtagac gattcgtgtg
     2281 cttgtgttcc gacatgtccc cttgtccttt ctttttgatt gcctccattc tacagccacg
     2341 cgcgcccatt gtaacttcga caagcgcgtt gattttgtta tactgcaaaa ctgaatggtg
     2401 attcattttt ataagtcctg aaacggacgg gcatgctcca ggtttgttca gctagcgggg
     2461 tgattttgat ggaacgagag agtttggaga tggatgtggt gttcgtcggg gcaggtccgg
     2521 cgaacttgag cggcgcgttg cacctggcgc gtctggtgaa ggagcacaac gagggggttg
     2581 cggcggggaa gcgcgagggc aagccgctcg gcgaacttga gatcggcgtg atcgaaaagg
     2641 gggcggcggt cggctcgcac atcctgtcgg gcgcggtgat ggacccgaaa gcgttgcgcg
     2701 agttgattcc tgatttcata gagcagggcg cacccgtcga atcgcccgtc aaagaagacg
     2761 agtttctgta tctcacggag acgcgtgcga ttcgttcgcc catcacgccg ccgccgctca
     2821 aaaaccacgg ctactacatc gtctctctga acaaactcgt cgcgtggctc ggcgaacaat
     2881 gcgaagcggc gggcgtcaat atcttcccgg agtttcccgg tgcggagatg ctctacgacg
     2941 gggaagaccg cgtcgtcggc gtgcgcacgg gcgacaaggg catcgacaaa gaagggaagc
     3001 gcaaggcgaa cttcgagccg ggcgttgacc tgttggcgaa agtgactgtg ctcggcgaag
     3061 ggccgcgcgg ttcgctcacg aagagtctgg cgcaacgtct gaatctcaac gatggacgcg
     3121 agccgcaggt ttatagtctc ggcatcaagg aactctggga gttgccggac gaccgctacc
     3181 cggcggggcg cgtcacgcac acgctcggct atccttccga cgcgcacacc tacggcggcg
     3241 gttggattta cggcatgcaa aatcgcatcc tcaacctcgg ctatgtgact ggcctcgact
     3301 accgcgaccc tctgctcgac ccgcacgcgg agtttcaacg cttcaaacag catccctttc
     3361 tcgccaaact tctggaaggc ggaaagctcc tgcgctacgg cgcgaagacc atcgccgccg
     3421 ggggctacta cgcgatgccg caactctacg cggacggcgt actgctcgtc ggcgactgcg
     3481 cgggctttct caattcgcaa cgcctgaagg gcatccacac ggcgatcaag agcggcatgc
     3541 tcgccgccga agcgatcttt gaagcactcg tggcaggaga tttttcggcg cgccagctac
     3601 agcgttacga aacgctcgtc aaagagagtt ggatcatgcc ggagttgcgg aaggtgagaa
     3661 atttccacgc gggttttcgc agaggccgct ggctgggaat cgccaatgcg ggtttgcagt
     3721 ttctcacggg cgggcgttcg tggggagtgt ttgatcgtgc ggaagcggag tccggccatg
     3781 ccgcgctcaa aaaactttcc gacttcggct acaaaggcga cgcgctcgaa caacgctata
     3841 aaggactccg cttcgacggc aaactgacct tcaataaact gacggacgtt tatcacgccg
     3901 ccgtcgggca cgacgaagat caacccgtgc atctgcacgt cctcgacacc aacatctgtg
     3961 tggagcgttg tgcggaagag tacggcaatc cctgccagcg tttctgtccg gcagcggtct
     4021 acgagatggt cgaagacccg gctacggcgg gcaagcggcg tttgcagatc aacttctcga
     4081 actgcgtcca ctgcaagacc tgcgacatca tggaccctta ccagatcatc aactgggtga
     4141 cgcccgaagg cggcggcggt cctgaataca agaacatgta aaagccgtga agcgagaagc
     4201 gtcaatcgtg tatggaaaga accgggtttt ctctatacac gattgacgct tctcgtttta
     4261 tgttcttatt gctatgcgac tcgcggaaga atctcaagcg cgcctcgaac aattttttcg
     4321 ttggtatgaa ggcgacgaac agttgcggct tccactgata ttcatccacg ccggattctt
     4381 gtcgcaccgc atggcgagag tattgcgcag cgcagccttc accgttggcc gtcacatctt
     4441 tgtctcacag gtctatgttg aaagagacga acgcggtcaa ctgacgatac ggggcgggct
     4501 gctcgcgcat gaagccacac acgtcaggca attccagagg gagggcttcc tcccttttct
     4561 ctacaattac ctgcgagagt atgtgacgtt tttattcaga accgggaagc tcaacgcgat
     4621 ggcgaggcga gcggcatacg agcagatcac gcgcgagagg gaagcgcgtg aagttgaggc
     4681 tgcttatctt gagtggcgac ttaacgcgac ctcttcaccg ggttaaaaaa agtcgtgagc
     4741 cgaaatcgcc gcctcactcc tgctccgtga tcgtgaaatc gacttttccc tgttccactt
     4801 ccaacagggc gatgcgcgtg tgtttgtgct gctgactgtc gatctcgacg cgggcgcgcg
     4861 cgccccgctg caattgcttg ctgcgctgtg cggcgacgat gatcatgcga tacttggagt
     4921 ccattgcggg cgtctgcttt tccgccgtcg cgggcgtgtt gtgatcttct ttgttcgatt
     4981 gagttgcttt agccatttac tttcccttcc gataaaacag attacttctt ttcggataag
     5041 cctaaaaagt tgttagttaa cgcgcaccgc tgcggcggtt acgtagcgtc tccgccggtt
     5101 catggaaacg tggcgagcac gcgctgtgcg aggcgttcct gccggggacg acgcgctctt
     5161 tcagcataca cgattccggc cagttgcgtc gccgcgcgcc ccacgtcgtc gttgagcacg
     5221 aggtagtcga actcacgata atcttccact tcgccgcgcg aattttgcaa acggacggcg
     5281 agagcttcgg agttctccga ttgtcgcccg atgagacgcc tgcgcaaaat ctcgaacgag
     5341 gggggcagga tgaagatgct cacggcctcg atccccgtgc gccggacggt ggccgcgccc
     5401 tgcacgtcga tctccaacac gatgtcgcgc cctgcggcca actcgcgctc gacaaccttg
     5461 agcgacgtgc cgtagagatt accgtgcacc aacgcccatt cgagaaattc gccgcgctcg
     5521 cgcgccgcat caaactcttc cgtcgtaacg aaatgataat cgcgcccgtt cgtttcgcct
     5581 tgtcgcggcg cacgcgtcgt ccacgaaacc gagtagccga ggcgcggcag gtgcgtcagg
     5641 atgcgacgaa tcagcgtgcc tttcccgccg cccgaaggcg aactgatgac aaagagaatc
     5701 ccgcgctccg gctgatcaag cgcgggcgac tcgtttgcgt ccatctgcga ccgctcactc
     5761 aacgttctgc acctgctcgc gcaacttttc cacttccgct ttgatcgcca gcgccgcctc
     5821 tttgatcgcc atgtcggtag atttcgagag caccgtattc gcttcgcggt taaattcctg
     5881 caagagaaaa tcgagccgct tgcccgtgtc gccttcgctg tctatgatct cgcggaactg
     5941 tttgacgtgg ctgcgcagcc gcgccagttc ttcgctgatg tcgctgcggt ctgcgagata
     6001 ggccacctcc tgcgcgagcc ttccctgatc gagttcgacg atgtcgagtc cgtcgcgcgc
     6061 cagcagttcg ctgatacgac gctgcaaacg cgccctgtaa gtttccacga gatcgctcgc
     6121 caccgcttcg atcacgggca cctgccgttc gatctcatcc aggcgcgcgt gcatctcggc
     6181 ggccaacgct tcgccttcga cgccgcgcat gtgattcaac tccgcgagag cctccatgac
     6241 ggcgcgctcg atgccttcgc gcatcgcctc cgtcaaactg ccgtcgcgca caggctgcaa
     6301 cgcgcccggc agacgcgcca gcacgttgat gtccggttcg cccgcgagcg aaaactcctg
     6361 ttgcaccgaa cgcatcgctt cgatgaaacc cgcgatgagc gggcggttca tttcataagc
     6421 gacttcgc
//
LOCUS       DASWNS010000006         8894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_415401, whole genome shotgun sequence.
ACCESSION   DASWNS010000006 DASWNS010000000
VERSION     DASWNS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8894
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415401"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VGB76_00095"
     CDS             complement(<1..628)
                     /locus_tag="VGB76_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097323.1"
                     /translation="MLTSLHKFDAKHAGQTASELDIIANGYRDFAGQLGELGEAETAE
                     YFADALGSASYLCRWIVATLGASSDAQSLLEAARARCLVLQERLNGSSHIGEAHSALN
                     LWIESILAVNNITQVSSALDSLKRIQLPVFYSRGNSKKQSSPSASASLETRATVKSPV
                     VKLMFSLDGQILATPQAIRSGMQHDLTIDMTVAAWPDSFDVLQVDYLST"
     gene            complement(655..3300)
                     /locus_tag="VGB76_00100"
     CDS             complement(655..3300)
                     /locus_tag="VGB76_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097324.1"
                     /translation="MMASKKSEDRLIEKSLEIGRKNAELMPKVRRWCKHLDIKMTSAG
                     ILAEVYNLPVGSMRVTCPHGTSLIESMHLSGVARSFILQNCVGCPHHEEISPDNFGRE
                     VFNEKAKADAQKEVDRAQREELKQRLYQGAQQALDTRDTPRRSINSLVLALQDSEPNA
                     PEYRDITDQLIQAAKVAPEHFSDEALRVLVAGDEETRLATGLHISRSICTFRKGVPDF
                     VLQAATKAVRQSFDVGGDAACGILLEHALINDLGDVLPLLQPIISVANYRLAHFLMSP
                     IIRSEPLFPNALELLRLVFAHKPEEARQAFDERLRRDNKATKLNAAKTLQAMLPDLAE
                     EVLPLTRVLLYSLELEDDHYEESADHEVCELISHLYAYSPQFVEVKIEAFWPVASDEV
                     KTLLTHVYSLIVRHASTEHRWSKPVFDEELYVRQVPTAIDHLFNAMADVRLDVEDRAE
                     ICDELEDAVKAFPQLAFDRLDRVLGRLAMTIREAEQIPKDGANYLEHMTNDTRRIEFD
                     RLIGHIVKITRTLIHHSPQKAFPVLTQTIHDLSSKNDTRLKAELVGILSDFGRDENLL
                     PQVVPELYRHLTDYESNLVRSRAIDVLSDLLDHSPGTVPSNMLELLRIYLQDPYVIIH
                     KSAVRALAHTELKRDDFGWDALNRVLALEKAYADEANQTAFFEDLIQTLYAAFRAWPE
                     VDRYIATKLLPKYSRHSEKYFAEDMLVRLSRSVEDYPGVAAEFVEIAVEYIRATSAKR
                     TASGDLYSDRSIIWQGLFEAPQEQLRPYIGVLREASIQKIQHEPLTSYQIIELLSHAG
                     LNKEALALAKDLLAALPDVKSNKYRRDTCCLIAAAEHAEQLCATGETEKAIQILVEAG
                     KAVPTESKLHEKLEEMWRAGLIETL"
     gene            3699..4562
                     /locus_tag="VGB76_00105"
     CDS             3699..4562
                     /locus_tag="VGB76_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097325.1"
                     /translation="MELIATTLGAGVTALVVGSIAGGLAAGVSAAVLTFIISLAAIFV
                     VHFLRAPSKLDSERQTEIDKLNAAVKDLRDSAGELEAKHQSELKALKKREKEIRFKLY
                     RRYRKILTDVKELLEPQVSIDYIADQPSFIALKTGGRKHYRVIRVRVTNTGGAPLYNL
                     RAQLKLTTRHTSYDNEDLTLKEEGLPIIRQILYRHEQHVLPRPQTSFNLSRGESQFVD
                     VAMQENEDGKWGTVTLCLSRIGVDNYSNIVNPSEPVEFSIVVLGDMSPCSRNFVLSLD
                     DVGVLQMKAAD"
     gene            4609..6216
                     /locus_tag="VGB76_00110"
     CDS             4609..6216
                     /locus_tag="VGB76_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEY0097326.1"
                     /translation="MSKVSSQPEPLVPPRRRNAKNLVRILAFSDWRVPDYEDLLKLAE
                     RSPGCDIVLYGGDDLDRVIEAREVVEKITCLTSAKKFLFVAGNDDSPHDRRALAATDF
                     AHDLHAEPFFYQDFAFIGLEGTTDGMGFIQHSESQVTEVLEGHLQTIRKRYGRKKPVT
                     VLVSHAPPRGVLDMAMRHSERPGARSIGSTSLRDFLEENRVPVTICGHVHLFGARQER
                     LPNKNLVINIASHDSRGAEGRFSVIDLDRSGHAEVSFHTTHVLLHDHELGRLQHVGMT
                     KVRRLLQNGIQSLADVTEENRPRLRIPGCGDRHINIWIRQSELIRGGFVGIEILEYDR
                     MAFLEDKRFVVWDIETDLKQTHIWLIGALDTLTGERRQFFNPDDESDCAKGFVEWMAG
                     RASATPVSFSCTRFDPRTLRKSLERHGILDAVNICERDVDLGNRLLYSCAHTYPTSKV
                     KDLAHHLGYRFRHPNLDGMAVGAMFSRYLNTGRGPGRWKPYLEYNEDDVLATLLILEK
                     VKEARARGETPRHKRRRRPPQRGAGRL"
     gene            6257..7189
                     /locus_tag="VGB76_00115"
     CDS             6257..7189
                     /locus_tag="VGB76_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097327.1"
                     /translation="MTELVALICAADRIPPSEIYQAGILLMFSCFNPDKTPGRRCEWK
                     GGNLQEFVSQYNRECGTTYVLVECLDAPKSVAQQAGLKQPEVLVEGSRGERPMVIERK
                     QVVTESYAAHHGNLHVLYKTVSESLAPHLGDALYVLEVTDAGLRGKWKGDVKAAVKEI
                     SDHVLANLQSARSGTAIGGQRPFPWRFSRVPAHARDDDAPEVGIGVQIHGSDTSADDP
                     KTLLRQVAEAYAETKKLLERVLAEAGPKFVAYQDHLKVVVLEFYGDWNLLDEDDAKRM
                     VGEVELPALIDEVWIARPEWVSEGDYEIGYDRAR"
     gene            7203..7604
                     /locus_tag="VGB76_00120"
     CDS             7203..7604
                     /locus_tag="VGB76_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097328.1"
                     /translation="MVKGRKKAGDLFRESTSLFGRKTSFAEAYPQIEDIEVEVEETGR
                     GVESRSRSRRYTKEYLPGEFVNCSNPMCYGGGFQLGLYLHGMVSDKKSELEESALCSG
                     NEGSPQGRRVYRKCLNLFRLKIKVTYKDSQG"
     gene            complement(7614..7958)
                     /locus_tag="VGB76_00125"
     CDS             complement(7614..7958)
                     /locus_tag="VGB76_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097329.1"
                     /translation="MADEIIVVKKSRHYKCVLSNAEMERMLANGVTFGGLGMYITMCE
                     FEPDTLFTVEDLTGLAPDTRDETEAILDGLINAGYVFRTDERVEGQAAYAVNRELLPE
                     SITEVPADQTVH"
     gene            8107..>8894
                     /locus_tag="VGB76_00130"
     CDS             8107..>8894
                     /locus_tag="VGB76_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097330.1"
                     /translation="MEMDTEDLPPVEGLKSEYLADGKISRAVNHHLSFYIWASGRDVP
                     AADPEFAALKLHLEEKGSVTRATARAIHNAAMKHLRNCGSRRAMETAFIPFFLGCWHP
                     LLQNKDMYEEMKEALRMLLSKALGDESLLRCAFKVGAYGYLSETAEEAGWELLEKYPA
                     DVDLVHPMAEHLAGDGRRAEAISVIERALRAGCADIEVLSTLARLRKEHKEREEFLSS
                     APERWPQVNYYQEHLLRLLAAGGPSDDFGALAKQSGIHHMSVWN"
BASE COUNT         2016 a         2403 c         2535 g         1940 t
ORIGIN      
        1 ttgtggaaag ataatccacc tgaagcacat cgaacgagtc cggccaggcc gccacggtca
       61 tgtcaattgt caggtcgtgc tgcatgccgc tgcgtatagc ttgcggagtc gctaagattt
      121 gtccgtcaag cgaaaacatt aatttcacga cgggcgactt cactgtcgcc cgtgtctcta
      181 aagacgcact agctgaaggg ctgctttgtt tttttgagtt accgcgcgaa taaaagacgg
      241 gcagttgaat gcgctttaaa gaatcaaggg cagatgaaac ctgcgtgatg ttatttacgg
      301 cgaggatact ctcaatccat aagttcaacg cgctgtgagc ctcgcctata tgggaagagc
      361 cattaagtcg ttcttgcagg actaaacagc gcgccctagc cgcctcgaga agtgactgtg
      421 cgtcggatga agctcctaac gtagcgacaa tccagcggca gagatatgat gccgacccaa
      481 gcgcatcagc aaaatactct gccgtttctg cctcccctaa ctcgccaagt tgaccggcga
      541 aatccctgta cccgttagca ataatgtcta gttcagacgc tgtctgacct gcgtgtttag
      601 catcaaactt atgcagtgat gtcagcacgc ggctcctcag agcgatggca tatttcagag
      661 tgtctcgatc aaaccggcgc gccacatctc ctccaatttc tcgtgtagtt tactctccgt
      721 ggggactgct ttacctgcct cgaccaaaat ctgtatcgcc ttctctgtct cccctgtcgc
      781 acacaattgt tcggcatgct cggccgctgc gatgaggcag catgtatctc tacggtattt
      841 gttagactta acgtcaggaa gggctgctaa aagatctttt gctaaagcaa gtgcctcttt
      901 attcaatccc gcatgcgata ggagctcgat gatttgatac gacgtgaggg gttcgtgctg
      961 aattttttgg atgctggctt cacgtaagac tccaatgtat ggccttagtt gttcctgagg
     1021 agcttcgaat aagccttgcc aaattatgga gcggtcagaa tagaggtctc cgctggctgt
     1081 gcgtttagcc gaagtagccc gaatatattc aaccgcgatt tcgacgaatt ctgctgcaac
     1141 tccagggtaa tcctcaaccg aacgagacaa tcttaccagc atgtcttcag caaaatactt
     1201 ttccgaatgc ctactgtact tcggtaaaag tttagtggct atatatcggt caacttcagg
     1261 ccaggcgcgg aatgcagcat agagcgtttg aattaaatcc tcaaaaaaag cagtttgatt
     1321 cgcctcatcc gcgtacgcct tttcaagagc caaaacccgg ttcaaagcat cccatccgaa
     1381 atcgtcccgt ttgagttccg tatgagctag tgcgcgcacg gcactcttgt gaatgatgac
     1441 gtatgggtct tggaggtaga tgcgcagcag ttccagcatg ttacttggaa cggtacccgg
     1501 agagtggtca agtaaatctg acagaacatc gatagctcga ctccgcacca aattggattc
     1561 gtagtcggta agatgtcggt agagctcagg tacgacctgc ggtagaaggt tctcatctct
     1621 tccaaaatcg ctaaggattc cgactaattc ggccttaagt cgcgtgtcat ttttactact
     1681 cagatcatga atggtttgcg ttaaaaccgg aaatgccttc tgtggggaat ggtgaatgag
     1741 cgttcgagta atctttacga tgtgtccaat caacctatca aactcaatgc ggcgcgtatc
     1801 gttcgtcata tgctccagat agttagcgcc atccttcggt atttgctccg cttcacgaat
     1861 tgtcatcgct agacggccta gaactcggtc tagcctgtca aatgctaact gagggaatgc
     1921 ctttacagca tcttcaagct catcgcagat ctccgccctg tcttcaacgt ctagacgcac
     1981 atcagccatc gcgttaaaga ggtgatctat tgcggtgggc acctgccgga catatagctc
     2041 ttcatcaaat actggtttcg accagcggtg ctcagtcgat gcatgtcgga caattaacga
     2101 gtagacgtgc gtgagcagcg ttttgacttc gtccgaggcc acaggccaga aggcttctat
     2161 ctttacttcc acaaactgcg gagagtacgc atataggtga gagattaatt cacatacttc
     2221 gtggtccgcc gactcttcat aatggtcgtc ctcaagctca agggagtaga gcaagactct
     2281 agtgagcggc aaaacctcct ccgcgaggtc tggaagcatc gcctgtagcg tcttggcagc
     2341 gttcagcttc gtcgctttat tgtcacgccg taatcgttca tcaaaagctt gtcgggcctc
     2401 ttctggttta tgtgcaaaga ctagccgaag aagctcaaga gcatttggaa atagcggctc
     2461 actgcgaatg atgggtgaca ttaagaaatg agccagcctg taattcgcga ctgatatgat
     2521 tggctgcagt aggggcagca cgtcacccaa gtcattgatc aaggcatgtt cgaggagaat
     2581 gccgcatgcg gcatcacctc ccacgtcaaa agattggcgg actgctttgg tagcagcttg
     2641 taaaacaaaa tcgggcacgc ctttgcggaa ggtacatata gaacgtgata tgtgcagccc
     2701 tgttgctaga cgagtttcct catcgccagc cacaagaact cgcaaagctt catcagaaaa
     2761 atgctctggg gcaaccttcg cggcttgtat aagttggtcg gtgatgtccc ggtattcggg
     2821 ggcgtttggc tcactgtctt gaagcgccaa aaccaacgag ttaattgacc gtcgcggcgt
     2881 gtctcgggtg tctagtgcct gttgtgcccc ttgatataac cgttgtttga gttcttctcg
     2941 ctgtgctctg tccacctcct tctgggcgtc agccttcgct ttctcgttaa aaacttcgcg
     3001 gccgaaatta tcgggtgaaa tctcttcatg atgcggacaa ccaacgcaat tttgcagaat
     3061 aaaggagcga gctactccag acagatgcat ggactcgatc aaagatgtgc catgtgggca
     3121 ggttacacgc atactgccga ccggtaggtt gtacacctcg gctagtatgc cggctgaagt
     3181 catcttgatg tccaagtgct tgcaccatct acggactttg ggcatcagtt cggcgttttt
     3241 acgacctatt tctaatgatt tttcgatgag ccggtcttca ctctttttgc tggccatcac
     3301 gtcctcttac agtagtgact taccactatt tgtggcccct tataagcgcc tatttattct
     3361 agatcatctt aacctaatgg actttgcctt tcaccaatac ctacaggcct gtttcctgaa
     3421 ccgaatccag cgatcggctt taccttataa gttcaaaaat tactcgactg ttgaggcgat
     3481 ccctccaatt gccttaatag gcacttcggt tcaattgaaa agttccggtt ctgcacacat
     3541 agtcttgtgg taggctttga gccgcaatgt ctgttgaaat gttacctcct aatacgccga
     3601 cagaggcgcc gcagcaaggg acaacacctt cccccgaaaa gccttacctc cgtagacgct
     3661 cggagtatgc agcccggcag tggcgagaac accaaaaggt ggagttgatc gccaccaccc
     3721 tcggggcggg cgtgaccgcc ctcgtggtcg gtagcatagc gggcggtttg gcggccgggg
     3781 tgagcgcggc cgtactgacg ttcattattt cgctggccgc catcttcgtc gtccacttcc
     3841 tgcgcgcccc ctcaaagtta gatagtgaaa ggcaaacaga gattgataag ttgaacgcgg
     3901 ctgttaagga tctgcgggac tcggccgggg agttggaggc taaacatcag tcagaactga
     3961 aggcgctgaa gaagagggag aaggaaataa ggtttaagct gtaccgccgc taccgcaaaa
     4021 tactgaccga cgtgaaagaa cttctggagc cgcaggtttc catcgactac attgccgacc
     4081 aaccgagctt catcgctctc aaaacaggag ggagaaagca ctacagagtt atccgcgtgc
     4141 gggtgacgaa taccggcggc gctccacttt ataatctgag ggcgcagctt aaattaacca
     4201 ctcggcacac gtcctatgat aatgaagact taaccctgaa ggaggaaggc ctgccgatca
     4261 tccggcagat tctttacagg cacgaacagc acgtgttgcc aagaccccaa acctctttca
     4321 acctgagccg gggagaatct cagttcgtcg acgtcgccat gcaagagaat gaagacggca
     4381 agtggggcac cgtgacgttg tgtctttcgc gcatcggggt agacaattac agcaatattg
     4441 taaacccgag tgagccggtc gaatttagca tcgttgtctt gggcgacatg tcgccatgct
     4501 ctaggaactt tgttttatcc ttagatgatg tgggggtgct gcagatgaaa gcggccgatt
     4561 aggcccctcc aaattacccg gcgcacttga gccagggagg aactctgcgt gagcaaggtt
     4621 tcgagccagc cggagccatt agtcccgccg cggcgaagaa acgctaagaa cctcgtccgc
     4681 atactggcct tcagcgactg gagggtcccg gactatgagg acttgctgaa gctcgccgaa
     4741 cgtagccccg gttgtgacat cgtcctctac ggcggtgacg atttagatcg cgtaatcgaa
     4801 gcacgtgaag tcgtcgagaa gatcacctgc ctgacgtccg ccaagaagtt cttattcgtg
     4861 gccgggaatg atgactcgcc ccacgacagg cgggcgctgg cggcgacgga ctttgcccac
     4921 gacctgcacg cggagccatt cttctatcag gattttgcct tcatcggact cgaaggcacg
     4981 accgacggca tgggcttcat ccaacactca gaatcccaag tgacagaagt cctagagggg
     5041 catcttcaga cgataaggaa gcggtacggg aggaaaaaac cggtcactgt tctcgtgtcc
     5101 cacgcgccgc cacggggcgt cctcgacatg gccatgcgtc attcggagag gccgggcgcg
     5161 aggtccatag gctctacgag cttgcgcgat ttcctggagg agaatcgcgt ccccgtcacg
     5221 atctgcggcc acgttcacct gttcggcgcc aggcaggaac ggctgcctaa caagaacctg
     5281 gtcatcaaca ttgccagcca cgacagccgc ggcgcggaag ggcggttctc ggtaatcgac
     5341 ttggaccgct ccggccacgc ggaggtttca ttccatacga cgcacgtcct gcttcacgat
     5401 cacgaactgg gcaggctcca gcacgtcggg atgacgaagg tgcggaggct tttgcagaac
     5461 ggcatccagt ccctggcgga cgtgacggag gagaacaggc ccaggttgcg catcccgggc
     5521 tgtggggaca ggcacatcaa catttggatc cgccagtcgg agctgattag gggcggcttc
     5581 gtcgggatag agatactcga atacgaccgg atggccttcc tagaggacaa gcgcttcgtc
     5641 gtctgggaca tcgagactga cttgaagcag acgcacatct ggctcatcgg ggccctcgac
     5701 accctaacgg gcgaaagaag gcagttcttc aacccggacg acgagagcga ctgtgccaag
     5761 ggcttcgtgg aatggatggc ggggcgggcg tcggcaactc ccgtcagctt cagttgcaca
     5821 cgcttcgacc cccgcaccct acggaagtcg ctcgagaggc acggcatcct ggacgcagta
     5881 aatatttgcg agagggacgt ggacctcggt aacagactgc tttatagctg cgcccacacg
     5941 tatcccacca gcaaggtgaa agacctcgcc caccatctcg gttacaggtt ccgccacccc
     6001 aacctggatg ggatggcggt aggcgcaatg ttctcccgct acttgaacac ggggcgcggg
     6061 cctggccggt ggaaaccgta cctggaatat aacgaggacg atgtcctggc cacactgctg
     6121 atactggaga aggttaagga ggccagggcc cggggagaaa cacctcgtca taagcgccgt
     6181 cgacgaccac cacaaagggg agccggtcgc ctgtaagcac ctgaggtgac taccggcgca
     6241 gataagaaga taattgttga cggagcttgt cgctcttata tgcgcggcag atcggattcc
     6301 accctcggag atttaccagg cagggatttt gctcatgttt tcttgtttta atccggataa
     6361 gacgcctggc cgcaggtgcg agtggaaggg cgggaacctg caggagttcg tcagccagta
     6421 caaccgggag tgtggcacta cgtacgttct ggtggagtgc ctggacgccc cgaagtcggt
     6481 ggcgcaacag gctggcctca agcagcccga ggtcctagtg gaaggtagcc gcggcgaaag
     6541 gccgatggtt atcgagcgca agcaggtggt caccgaatcc tacgctgcac accacggtaa
     6601 tctgcatgtc ctgtacaaga cggtctcgga gtctctggcg cctcacttgg gggacgcgct
     6661 gtacgtctta gaggttacgg acgccggcct gcggggaaaa tggaaggggg atgtgaaggc
     6721 ggcggtcaag gagatctccg atcacgtcct cgctaacctg cagagcgcca ggagcggcac
     6781 ggccatcggc ggccagcggc ccttcccctg gagattcagc cgggtaccgg ctcacgcgcg
     6841 ggatgacgac gcccccgagg tcgggatcgg cgtgcagatt catggctcgg acacctcagc
     6901 ggacgacccg aagacattac tgcgccaggt cgcagaggcg tatgcggaaa cgaaaaaatt
     6961 gttagagcgg gtactcgccg aggcggggcc gaagtttgtc gcctaccaag atcacctcaa
     7021 ggtcgtggtg ctggagtttt acggcgactg gaacctcttg gacgaggacg atgcgaagag
     7081 aatggtcggg gaggtcgagc tgccggcgct gattgacgag gtgtggatcg cgcgccccga
     7141 gtgggtctcc gagggggatt atgagatcgg ctacgaccgc gcccgctaga cgtgggaggc
     7201 gaatggtgaa gggacggaag aaagccggcg acctgtttcg tgagagcacc tcgctcttcg
     7261 gcaggaagac ctccttcgcg gaggcttacc ctcagatcga agacatcgaa gtcgaggtgg
     7321 aggagaccgg ccgcggcgtc gagtcgcgga gtcggagtcg gaggtatacg aaagagtatc
     7381 tgcccgggga gtttgtcaac tgcagcaacc cgatgtgtta cggcgggggg ttccagttag
     7441 gcctgtatct gcacggcatg gtctcggata agaagagcga gctcgaagag tcggcgctgt
     7501 gcagtgggaa cgagggctcg cctcagggcc ggcgcgtcta caggaagtgc ctgaacctgt
     7561 tcaggctgaa gatcaaagtc acgtataaag actctcaggg atgagcgacc tgatcaatgt
     7621 accgtctgat cggcggggac ttcggtgatc gactccggga gcagctcgcg gttcacggcg
     7681 taggcagctt ggccctccac tcgctcgtcg gtcctgaaga cgtacccggc atttatcagg
     7741 ccgtcgagga tggcctccgt ttcgtctcgc gtgtccgggg cgagccccgt caggtcctcg
     7801 acggtgaaga gcgtgtcggg ctcaaactca cacatcgtga tgtacatccc cagcccgcca
     7861 aacgtcacgc cgttggccaa catccgctcc atctcggcgt tagacaagac gcatttgtag
     7921 tggcggcttt ttttaacgac gatgatttcg tcagccacga tttgtgtctc ctgccccgcg
     7981 ggcggatatg gattattccc cgcgggctgt tatagagtag cctaccgctt caggccgggg
     8041 agcatagcag aaaatactct ggctggcagc cggcctcagc cgatctgatt ctcatacggt
     8101 tgccgcatgg aaatggacac agaagacttg cctcccgtcg agggcttaaa gtcagaatac
     8161 ctggccgacg gtaagatcag ccgcgccgtc aaccaccacc tcagcttcta catctgggcc
     8221 tccgggaggg acgtgcccgc cgccgacccg gagttcgcgg ccctcaaact ccacctcgaa
     8281 gaaaaaggct ccgtcacccg agcgaccgcc cgcgcgatcc acaacgccgc catgaagcac
     8341 ctgcggaatt gcggcagccg acgcgccatg gagacggcct tcatcccttt ctttcttggc
     8401 tgctggcacc ctctcctgca gaacaaagac atgtacgagg agatgaagga agcactgagg
     8461 atgttgctct caaaagctct gggggacgag agcctcctcc ggtgcgcgtt taaggtcggc
     8521 gcctacggat atttaagcga gacggcggag gaggcggggt gggaactgct ggaaaagtat
     8581 ccggccgatg ttgatctcgt tcacccaatg gccgaacacc tcgcaggcga tgggcggcgc
     8641 gccgaggcaa tctcggtcat tgagcgcgcc ctgcgggcgg gctgcgcgga cattgaggtt
     8701 ctgtcgactc tggcgcgact ccggaaagaa cataaggagc gagaagaatt cctgagctcg
     8761 gcgccggaac ggtggccgca ggtcaactac taccaggaac atctcctgcg gctgctcgcc
     8821 gctggcggcc cgtccgacga cttcggggcg ctcgctaaac agtccggcat tcaccacatg
     8881 agcgtctgga atca
//
LOCUS       DASWNS010000007         5272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_78177, whole genome shotgun sequence.
ACCESSION   DASWNS010000007 DASWNS010000000
VERSION     DASWNS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78177"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..2753)
                     /locus_tag="VGB76_00135"
     CDS             complement(78..2753)
                     /locus_tag="VGB76_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097331.1"
                     /translation="MPTIEHERLKDSPGAPVQPWRKWGPYVSERAWGTVREDYSADGS
                     AWDYFPHDLARSKAYRWGEDGLAGICDRYQLMTFALALWNGRDPILKERLYGLVSSEA
                     NHGEDVKEYYFYLDSTPTHSYMKYLYKYPQREFPYSQLIEENRARRGGGQPEYELLDT
                     GVFADDRYFDLFVEYAKATPEDICVRIEVFNRGAEDAPLHLLPHLWFRNIWAWWAEPS
                     AEPIISLGESGEDFVSLVASDEETCGLPGLPFKYCLGTRYLYGEPGGQPLFTDNETNA
                     PRVWGAHVESRKPYVKDGFHRHIINGEPTVNPEQRGTKACLHYEFNVPAQGSVVLRLR
                     LSDEKLEKPLADVDEIVQERRREADQFYAAIQPAKATEDERRVQRQAFAGMLWSKQIY
                     IYDVRAWFEGDNPAWPPPASRRHIRNTRWMHIKSMRVLSMPDKWEYPWFAAWDLAFHC
                     APLALVDPEFAKEQLWLLLFEQFQHPNGQIPAYEWEFSDLNPPVHAWAVWRIYNQDRI
                     ASGKADRLFLEKCFHKLLINFAWWVNKVDSEGNNVFEGGFLGLDNITVLDRSHELPNG
                     ATLEQSDATGWMGMFCLNLMRIALELAKENPAYEALATKFFQHYVYVGAAMKRMGSVG
                     YQLWDEDDRFFYDVLSYPNGEHHKFRVRSLVGLIPLYAIERLEENWIKPFTQFHREMR
                     WFIENRQDIVQSCVQIVEHEDQRVYVLAIVNQEQMRHLLGRIWDPQEFRSDYGLRSLS
                     KYHETHPFHFGGSQVGYEPAESVEMLKGGNSNWRGPVWFPTSFMMIEALRKVGKAYGH
                     KFEIESPVEGEPPVTLNDMAQGFANRLINIFTRDREQRRPVHGWYQKFQDDPHWRDYI
                     LFYEYFHGDNGMGLGASHQTGWTGLVASLIDEWRK"
     gene            complement(2953..3405)
                     /gene="rpiB"
                     /locus_tag="VGB76_00140"
     CDS             complement(2953..3405)
                     /gene="rpiB"
                     /locus_tag="VGB76_00140"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEY0097332.1"
                     /translation="MRPKRVAIGSDHAGYESKESAKREIAALGVEVEDKGTNSLESVD
                     YPDFGAAVGRAVASGEVERGVVICGSGIGISIAANKVPGVRAALCWNEETARLARQHN
                     DANVLCIGARFIEPELAARMIRAFLETDFDGGRHQERVEKLTQLDGSR"
     gene            complement(3492..4250)
                     /gene="pgl"
                     /locus_tag="VGB76_00145"
     CDS             complement(3492..4250)
                     /gene="pgl"
                     /locus_tag="VGB76_00145"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY0097333.1"
                     /translation="MPADRATPNLQIFADATGVARAAALRFIEIARESTQARGRFSVA
                     LAGGSTPKRVYEMLAGEELSRELDWSQVHIFFGDERTVPPDDAESNYRMANEALLARV
                     PLPEQNIHRMRGEGDAVANARLYEDELRGFFDGEDWPRFDLVMLGMGDDGHTASLFPE
                     TRALDVQAAWVTANWVEKFDTFRVTLTVPAINHARHVLFIVTGASKAARLQEVLYGAR
                     EPRRLPSQLIQPHEGALAWFLDEAAAKLLRKDEG"
     gene            complement(4275..5252)
                     /gene="glk"
                     /locus_tag="VGB76_00150"
     CDS             complement(4275..5252)
                     /gene="glk"
                     /locus_tag="VGB76_00150"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY0097334.1"
                     /translation="MILAGDIGGTKTNVALFEIGQTGKPLAQRSFPSNKYDSLESIVA
                     EFVAEHQPQLTHACFGIAGPVKDGQVKTPNLKWIVDAQTLAQTLKLKRVNLINDLEAT
                     AYGVEGLSEAQLHTLNEGHEEHGGHRALIAAGTGLGMAGLFYDGKHYRPIASEGGHID
                     YAPRTAIEHELLNYMRAQVGGRVSYERVLSGPGLFNIYSFLRDHKYGEEPVWLAEEIK
                     AGDGSAAVSIAGLNGKSELAVKALDLFAEIYGSMAGNLALILKATGGLYIGGGIAPKL
                     IGKLKDGTFMRAFRDKGRMSALVSNVPVRVILDDKAALYGAARCAVEVI"
BASE COUNT          988 a         1592 c         1565 g         1127 t
ORIGIN      
        1 aggacgcgag agaagagaca ctcttctctc gcgtccttcg gggtttcgac ttcgcgtgct
       61 ttagcgtccg gttcccttca cttgcgccat tcgtctatca acgaggcaac cagcccggtc
      121 cagcccgtct gatgcgacgc gccgagtccc attccgttgt cgccgtgaaa atactcgtaa
      181 aagagaatgt agtcgcgcca gtgcgggtcg tcctgaaatt tttgatacca gccatgcacg
      241 gggcgacgtt gctccctgtc gcgcgtgaag atattgatca ggcggttggc gaaaccttgc
      301 gccatgtcgt tgagcgtcac gggcggttcg ccttccaccg gactctcgat ctcaaacttg
      361 tgcccgtagg ctttgcctac tttgcgcaaa gcctcgatca tcatgaagga agtgggaaac
      421 cagacgggac cacgccagtt ggagttgccg cccttcaaca tctcgaccga ctctgccggt
      481 tcataaccca cctgactgcc gccgaaatga aacgggtgcg tttcgtgata tttcgacagg
      541 ctgcgcaacc cgtaatcgga acgaaactcc tgcgggtccc agatgcgccc gagcaggtgt
      601 cgcatctgct cctgattgac gatggcgagc acgtagacgc gctggtcttc gtgctcgacg
      661 atctggacgc aactctgcac gatgtcctgc cggttctcga tgaaccagcg catctcgcgg
      721 tgaaactgcg tgaagggttt gatccagttc tcttcgagcc gctcgatggc gtagagcggg
      781 atgaggccga cgagcgagcg cacgcggaac ttatgatgct cgccgttcgg gtaactcaac
      841 acgtcgtaga agaagcggtc gtcttcgtcc cacagttgat aaccgacgct gcccatcctc
      901 ttcatcgccg cgccgacgta gacgtagtgc tggaaaaatt tggtggcgag agcttcgtag
      961 gccggattct ctttcgccag ttcgagcgcg atgcgcatga ggttgaggca gaacattccc
     1021 atccagcccg tcgcgtcgga ttgttcgagc gtcgccccgt tcggcagttc gtggctgcgg
     1081 tcgagcaccg tgatgttgtc gaggccgagg aagccgcctt caaagacgtt gttgccttcg
     1141 ctgtcaacct tgttgaccca ccatgcgaag ttgatcagca acttgtgaaa acacttttcg
     1201 agaaacagac ggtcggcctt gcccgaagcg atgcgatcct gattgtagat gcgccacaca
     1261 gcccacgcgt ggacgggcgg gttgagatcg gagaactccc attcgtaggc cggaatctgc
     1321 ccgttcggat gttgaaactg ttcgaagagc agcagccaca actgctcttt cgcaaactcc
     1381 gggtcaacta acgccagcgg cgcgcagtga aaagccaggt cccacgcggc aaaccaggga
     1441 tactcccact tgtccggcat cgaaagcacg cgcatgctct tgatgtgcat ccagcgcgtg
     1501 ttgcgtatgt ggcggcgcga ggcgggcgga ggccacgcgg gattatcgcc ctcgaaccat
     1561 gcgcgcacgt cgtagatata gatctgcttg ctccagagca tccccgcgaa cgcttgccgc
     1621 tgcacgcgcc gctcgtcttc ggtcgccttc gcgggttgga tcgccgcata aaattggtcg
     1681 gcttcgcgtc gccgctcctg cacgatctca tccacgtccg cgagcggctt ttccaacttc
     1741 tcatccgaca ggcgcagtct taaaacgaca gacccttgcg ccgggacgtt aaactcgtaa
     1801 tgcaggcaag ccttcgtccc gcgctgctcc ggattgacgg tcggttcgcc gttgatgatg
     1861 tggcggtgaa acccgtcttt gacgtaaggc ttgcggcttt cgacgtgtgc gccccaaacg
     1921 cgcggcgcgt tcgtctcgtt gtccgtgaag agtggttggc cgcccggctc gccgtagaga
     1981 tagcgcgtgc cgaggcaata cttgaagggc aggccgggca gcccgcaagt ctcttcgtcg
     2041 gaggcgacga ggctcacaaa gtcttcgccg ctctcgccga ggctgatgat cggttcggca
     2101 gacggctcag cccaccaagc ccagatattg cgaaaccaga gatgcggcag gagatgaagc
     2161 ggcgcatctt ccgccccgcg attgaagact tcgatgcgga cgcagatgtc ttccggcgtg
     2221 gccttcgcgt attcgacgaa gagatcgaag tagcggtcgt ctgcaaagac gcccgtgtcg
     2281 agcagttcgt attcgggttg accgccgccg cgccgcgcac ggttctcctc gatgagttga
     2341 ctgtaaggaa actcgcgctg cgggtacttg tacaggtact tcatgtacga gtgggtcggc
     2401 gtcgagtcga gataaaagta gtactccttc acgtcctcgc cgtgattggc ttcgctggag
     2461 acgaggccgt agaggcgttc tttcaagatc ggatcgcgcc cgttccagag tgcgagcgcg
     2521 aaggtcatca gttgatagcg atcacaaatt ccggccagcc cgtcctcgcc ccagcgatac
     2581 gccttcgagc gcgcgaggtc gtgcggaaag tagtcccacg cagagccgtc cgcgctgtaa
     2641 tcctcgcgca ccgttcccca cgcgcgctcc gagacatacg gcccccactt gcgccacggc
     2701 tgaacaggcg cgcccggaga atcttttaat cgttcgtgtt ctatggttgg catggaaatt
     2761 tgagtaaact gaatttcaag tcgggcagaa caatctcacg acgcgggcgc aaagacgcct
     2821 gacgaaaggc gcggagagaa cctgtcttaa ctttgcggct tcactttcga cttcactttc
     2881 gcctctgtgc ctgcgcgtga aatgcccgct cgcagtgcgg cctcacactt tgacgtgtgg
     2941 cttcctcacg cttcaacgcg acccgtccaa ctgcgtcaac ttctccacgc gctcttgatg
     3001 tcgcccgccg tcgaagtccg tctcaagaaa ggcgcgaatc atgcgtgcgg cgagttcggg
     3061 ttcgatgaag cgcgcgccga tacagagcac gttggcgtcg ttgtgctggc gcgcgagtcg
     3121 tgccgtctct tcgttccagc agagcgcggc gcgcacgccc ggcactttgt tggcggcgat
     3181 agagatgccg atgcccgaac cgcagatgac gacgccccgc tcgacttcac cgctcgccac
     3241 cgcgcgcccg accgccgccc cgaagtctgg gtaatccacg ctctcaaggc tgttcgtccc
     3301 cttgtcttca acttcgacgc cgagcgcggc aatctcgcgc ttcgccgatt ccttgctctc
     3361 atacccggca tgatcgctgc cgatggcgac acgttttggc ctcataaaaa acagggatga
     3421 aggatgaggg atgagggatg aacaagaaaa gcactcgcgc tctcaggtcg tctcctttca
     3481 tccctcatct ctcatccttc atcctttctt aagagtttgg cggcggcttc gtccagaaac
     3541 catgcgagcg cgccttcgtg cggttgaatg agttgtgaag ggaggcgtcg gggttcgcgt
     3601 gcgccgtaca ggacttcctg caaacgcgcc gccttgcttg cgcccgtgac gatgaacagc
     3661 acatggcgcg cgtgattgat ggcgggcaca gtcagcgtca cacgaaaggt gtcgaacttt
     3721 tcgacccagt tggccgtgac ccatgccgcc tgcacgtcga gcgcgcgtgt ctcgggaaag
     3781 agcgaggcgg tgtggccgtc gtcgcccatg ccgagcatga cgagatcgaa gcgcggccag
     3841 tcttcgccgt cgaagaagcc gcgcagttcg tcttcgtaga gacgcgcatt ggcaaccgcg
     3901 tcgccttcgc cgcgcatgcg atggatgttc tgttcgggca gcggaacgcg cgcgagcaaa
     3961 gcctcgtttg ccatgcgata gttgctctcg gcatcgtccg gcggaacggt gcgttcgtcg
     4021 ccgaaaaaga tgtggacttg cgaccagtcg agttcgcggc tcaactcttc gcccgcgagc
     4081 atttcgtaga cgcgcttcgg cgtggagcct ccggcgagcg cgacgctgaa acggcctcgc
     4141 gcctgcgtgc tctcccgcgc gatctctatg aaacgaagcg cggcggcgcg tgctacgccg
     4201 gtcgcgtccg caaagatttg taaatttgga gtcgcacggt cggctggcat agagttgtcg
     4261 aaatttgaag tcgatcaaat aacttcgacg gcgcaacggg ctgcaccata gagcgcggcc
     4321 ttgtcgtcga ggatgacgcg cacgggaacg ttgctgacga gcgcggacat gcgcccttta
     4381 tctcggaagg ctctcatgaa ggtgccgtct ttcaatttcc cgatcaattt gggcgcgatg
     4441 ccgccgccga tgtagaggcc gcccgtcgct ttcaggatca aggcgaggtt gcccgccatc
     4501 gaaccgtaaa tttctgcgaa cagatcgaga gctttgacgg ccaactccga ctttccgttc
     4561 agcccggcga ttgagactgc cgccgaaccg tcgcccgctt tgatctcttc ggccagccac
     4621 acgggttctt cgccgtactt gtggtcacgc aggaaactgt agatgttgaa aagcccggga
     4681 cccgacagca cgcgttcgta gctgacgcgc ccgcccacct gtgcgcgcat gtaattcagg
     4741 agttcgtgtt cgatggcggt gcgcggggcg taatcaatgt ggccgccttc ggaggcaatg
     4801 gggcgataat gtttgccgtc gtaaaaaagc ccggccatgc cgagtcccgt cccggcggcg
     4861 atgagcgcgc ggtgcccgcc gtgctcctcg tgcccttcgt tgagcgtgtg cagttgcgct
     4921 tcgctcaagc cttcgacgcc gtaggctgtc gcttccagat cattgatcaa gttgacgcgc
     4981 ttcagtttga gagtttgcgc aagcgtctgc gcgtctacga tccacttgag attcggcgtc
     5041 ttgacctgac cgtctttcac gggtccggcg atgccgaaac aggcgtgcgt caattgcggc
     5101 tgatgctctg cgacaaactc cgcgacgatt gactccagcg agtcgtactt gttgctcgga
     5161 aaacttctct gcgcgagcgg tttccccgtc tgtccaattt caaagagggc gacgttggtt
     5221 ttcgtccctc cgatgtctcc ggcaagtatc atgtcgttaa aaaattaatt ct
//
LOCUS       DASWNS010000008        10406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_363606, whole genome shotgun sequence.
ACCESSION   DASWNS010000008 DASWNS010000000
VERSION     DASWNS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363606"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2772)
                     /locus_tag="VGB76_00155"
     CDS             complement(<1..2772)
                     /locus_tag="VGB76_00155"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0097335.1"
                     /translation="MKKKLTLHLAVVLLGAGLLTGVHAQTSTVGSISGSVRDPQGAAV
                     PKTEVVITEETTGQTRTVKTDDDGDYSAQSLPIGRYSVSASPQGFKKTVATGVEVHVG
                     DSLVVDLKLEVGAVTETVTVTDAARLVETESGKVSSLISEKQVTELPLNGRNYAALVT
                     LVPGLSAPNEGGAFGTRGTGLDSHVDVSVNGNQSNANMWTVDGVNNMDVGSNATLLVF
                     PSIDSIAEFRVERNSFSAEYGQAQGAVINLITKGGGNEFHGSLFEFFRNDKLNANDWF
                     ANRSGNYGPNSPEVQLGLKQVGDEITPRGQLRYNNFGGNFNGPIIKNRVFFFWSEEWR
                     RERRGTPLTSRVPTAQERLGDFSGTLTGPVPRDPLTCQRNPANGLLVRDANGQPVGCQ
                     PFPGNRIPQNRLSPAGLALMNIFPLPNNAADPTGTNWISAPLQPVDTRQDLIRGDITI
                     TDKMNLMVRYINETWVHGQAAGNFWGDTPFPTLASDWEQPSQSFAIKLTNTLSSTAVN
                     EFQFSRAGNDIFVTTNPASTALNENIASTFPTVFSRVQGTGLPTVGWGAGGYPTLWHQ
                     APWDNHQDLFIWKDDFSKVLGSHNTKFGVLFSHNIKNEKPNGGSGLYTIQSDGGRTGN
                     AIAELLLRDLPILQYTEFENQGTTLGRWHDFEFYGTDTWKARPNLTLTFGVRYSLFPP
                     AYADDNRITNWIPELYDGQTLSTGFVRADQAGQAGLPRALVNTYKGGIQPRLGLAWDV
                     HGNGKTAVRMGFGRYMSRSNVIASLLRLSNNPPWNTVVDSGWNPDAVTLADCPNCRSL
                     DTINPGLRNSAAGLGGVNSVDPNFKPPESWQWNLTVSREVMKNTVAEVSYVGNHGLHL
                     WRLINGNYNQVLPQFRAAVAAGTRSADSSRRFGFTNGVTRDESTGDSNYHGLQVWIDR
                     RFTNRLAFQT"
     gene            3352..4719
                     /locus_tag="VGB76_00160"
     CDS             3352..4719
                     /locus_tag="VGB76_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097336.1"
                     /translation="MELGKEQKLEQLEKVLHSRTLQNSESLKAFLRFVVEKTVADEDV
                     LLKEYTIATEVFGRNGDYDPRIDSVVRVQAGRLRTKLQEYYTTEGKADPVIIDLPKGH
                     YHPVFNCPHPPAAIETATGVTADSQTTTEAARAADAQAQAGSAHARGRGRALTFLLAA
                     LVVVLVVAVAALYNSNRELRRQASNADAALDPADFKAVWSPFISDPESPLLVLSNPTV
                     YRFLNEADPDSLEQRAIQLTPEQTRSLVDAPEFRGQWTGGDSPRLIPSLGMYTGMGEA
                     IGLYRLTDLFRTAQKTILLRQSRHASAADLKYRNVILLGSIYVNEWTRRLPTVESFNY
                     TFSATIENRDPQAGEEREYKPQFNPQTGELTVDYALITVKPNVSGEHAVMTLAGIFSE
                     GTEAAAEFVTTRNHLAVLGQRLRQLGGQNAPPKYYQALLKVEVENGTPTTITLLSLRA
                     LPEKQ"
     gene            complement(4949..7360)
                     /locus_tag="VGB76_00165"
     CDS             complement(4949..7360)
                     /locus_tag="VGB76_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY0097337.1"
                     /translation="MKRMFATYAFAALSLYALLPLHTSAARQQETAASARTATTTATA
                     SELTDSHTVFRRMLAPPASNAEAARRADELLSKMSLEEKVGQMTQLEISMVSTGRNQE
                     IKIDPAKLEKAVVKYGVGSILNVADQALTLDRWHDTIRQIQEAARRTRHKIPVLYGID
                     TIHGANYVQGATLFPQPLAMAATWNPELMERASAVAAIETRAAGIPWTFSPVLDTGRQ
                     PLWPRMWETYGEDPYLISVMGVASVRGYEGLNLSSSERVAACLKHYMGYGLPLSGRDR
                     TPAWIPEIYLREYVLPPFKAAVDAGARTVMINSGEINGVPVHASHFILTDILRKELGF
                     RGVAVSDWEDIKKLVNIHRVAATEKEATRMAVMAGVDMSMVPSDYSFADHLLALAREG
                     AVPQSRIDEAVRRILTLKFELGLFTDSMPDASLRAKFATPESRRMSLEAAREAMTLLK
                     NTNELLPLAKNRKVLVTGPTADSLISLNNGWTYVWQGSEESLYPKDRPTIRQAIEAKV
                     GKANMSYVAGTRIRRNGNAPSNVTPTDFDEEVDIAAAARAARDADVVILCLGEGSYTE
                     TPGNITDLTLPEPQLRLAEAVAAAGKPVVYVLVEGRPRIIARIAERAGAILLAYNPGN
                     EGGDAVADVLFGDYNPSGRLPFTYPRHPNALLNYDHKSYENESTTFGLTPFNPQWAFG
                     HGLSYTSFKYGDLRLSRKSLSEADKLGVSVTLTNTGRRAGSEVVQLYVRDLVATITPP
                     DKRLRRFAKVHLAPGQTRTLTFELERADLSFIGQNNQPVVEPGDFEVLVGGLSDKFTL
                     VTSGK"
     gene            complement(7583..8233)
                     /locus_tag="VGB76_00170"
     CDS             complement(7583..8233)
                     /locus_tag="VGB76_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097338.1"
                     /translation="MENQRASSSIIVAGFVAVVCLLTLAAHSARAQQTTPGGPPPPGQ
                     RDPFGEVRERQQREAQLRSAEMLGAVKSKDRRGAQAAAEQLREDFRNIQILRNKLVRH
                     LQSEKPLDYKFIAAETGEVNRRAGRMQTHLLREVAANEKKATETPVEIGDEQLKDALV
                     TMCKRIDSFTESPVFKVPEVVNVEQSAKANRDLRHIILLSGGIRRTAERLNKSHKK"
     gene            8696..10357
                     /locus_tag="VGB76_00175"
     CDS             8696..10357
                     /locus_tag="VGB76_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY0097339.1"
                     /translation="MKRFFASILVIALCSMMVSPGLVYSQRSNKAAKLARAEAASLLR
                     LQKKLARKGIAPNDVKTLRVFEDTDRAERVHTHVQQTHQGVPVFGGEAIVHLNAATEE
                     EAQPTTDTLIAGVSVSTTPGMTVGDAVRLAKSEYKVKKGCDECFTDAPQTDLWVLRHE
                     GEDHLVYRVQLSRLDGTEHTSEPVYFIDAHTGALVWSYDNLQTQAATGTGVSLYSGTQ
                     AINTFKSGTSYYMEDNARALGTFDNRNSAKINSMLGYITSYGSMYRFADADNFWDAAT
                     ARAGVDAHYGAAKTYDYFLNVHGRNGIDGAGGPRTLASADGSTGLISSIVHFGKAYNN
                     AFWSSTKNQMFYGDGDGTNFSPLVTLDITGHEMQHGITSRTANLTYANESGALNESWS
                     DVFGAMVELYAEGQETPTTWWIGEDANTPGVAGDALRYMDTPHKAGNYGYTTNDDPDH
                     YSERYTGTGDSGGVHINSGIPNHVFYLLAKGGTHAHIVGAPVTGIGADKAAKIWYKAL
                     TTYMTSSTNFTGARTATLDAANALYGVGSPESISVAQAWSACGIL"
BASE COUNT         1954 a         3164 c         3221 g         2067 t
ORIGIN      
        1 ggtttggaag gcgagccggt tggtgaagcg ccggtcgatc cagacttgaa gcccgtggta
       61 attggaatcg ccggttgatt cgtcacgcgt cacgccgttg gtgaaaccga agcggcgcga
      121 ggaatctgcc gagcgcgtcc cggcggccac ggcggcgcgg aactgcggca acacctgatt
      181 gtagttgccg ttgatgagcc gccagagatg gaggccgtga ttgccgacgt aggagacttc
      241 ggccacggtg ttcttcatca cctcgcgcga gacggtcaga ttccactgcc agctctcggg
      301 tggcttgaag ttgggatcga cggaattgac gccgcccaga cccgccgccg aatttctcaa
      361 ccccggattg atcgtgtcga gactacggca gttcggacag tcggccagcg tgaccgcgtc
      421 cgggttccag cccgaatcga cgaccgtgtt ccagggcgga ttgttagaga ggcgaagcag
      481 cgaggcgatc acgtttgaac gactcatgta acgaccgaag cccatgcgaa cggcggtctt
      541 accgttgccg tgtacgtccc aggccaaacc gagacgcggc tggatgccgc ccttgtaggt
      601 gttgacgagc gcgcgcggca gacccgcctg cccggcctga tcggcgcgga cgaagccggt
      661 gctgagcgtc tggccgtcgt agagttcggg tatccagttg gtgatgcggt tgtcgtcggc
      721 gtaggcgggg gggaagagcg aatagcgcac gccgaaagtg agcgtcaggt tgggacgcgc
      781 cttccaggtg tccgtgccgt agaactcgaa gtcgtgccag cgtccgagcg tcgtgccctg
      841 attctcaaac tctgtgtatt gaaggatcgg taagtctctg agcagcagtt cggcgatggc
      901 gttgccggtg cgtccgccgt ccgactggat ggtgtagagg ccgctcccgc cgttcggttt
      961 ttcgttcttg atgttgtgac tgaaaaggac gccgaacttc gtattgtgtg agccgagcac
     1021 tttggagaag tcgtctttcc agatgaagag gtcttggtgg ttatcccacg gagcctgatg
     1081 ccagagggtc gggtagccgc ccgcgcccca accgacggtc ggaagccccg tgccctgaac
     1141 gcgggagaag accgtgggga aagtcgaagc gatgttctcg ttgagggctg tgctcgcggg
     1201 gttggtcgtg acgaagatgt cgtttccggc gcgcgagaac tggaactcgt tcaccgccgt
     1261 cgaactcaga gtgttagtca acttgatggc gaagctctgg ctcggctgtt cccagtcgga
     1321 ggcgagcgtg gggaacggcg tgtcgcccca gaagttgccc gccgcctgac cgtgcaccca
     1381 cgtctcgttg atgtagcgca ccatcaggtt catcttgtcg gtaatggtga tgtcgccgcg
     1441 gatgaggtct tgccgggtat cgacgggctg caagggcgcg gaaatccagt tggtgccggt
     1501 cgggtcggcg gcgttgttcg ggagcgggaa gatgttcatc agggcgagtc ccgcggggct
     1561 taggcggttt tgcgggatgc ggttgccggg gaacggctgg cagccgacgg gttgaccatt
     1621 cgcatctctg acgagcaggc cgttggccgg gtttcgctgg caggtgagcg ggtcacgcgg
     1681 cacgggtccc gtgagcgtgc cgctgaaatc tcccagacgc tcctgcgcgg tcggcacgcg
     1741 ggatgtgagc ggcgtgccgc gacgctcgcg ccgccactcc tccgaccaga agaagaagac
     1801 gcgattcttg atgatggggc cgttgaagtt gccgccgaag ttgttgtaac gcagttggcc
     1861 gcgcggcgtg atctcgtcgc cgacctgctt gaggccgagt tgcacttcgg gagagttcgg
     1921 cccgtaattg cctgagcggt tggcgaacca gtcgttagcg ttcagtttgt cgttgcggaa
     1981 aaattcgaag agcgagccgt gaaactcgtt gccgccgccc ttcgtgatga gattgatgac
     2041 cgcgccctgc gcctgcccgt actccgcgct gaaactgttg cgctcgacgc ggaactcggc
     2101 gatggaatca attgagggga agaccaggag cgtggcgttt gaacccacgt ccatgttgtt
     2161 cacgccgtca accgtccaca tgttggcgtt cgactggttg ccgttgaccg aaacgtctac
     2221 gtgcgagtcg aggccggtgc cgcgcgtgcc gaacgcgccg ccctcgttcg gggcggagag
     2281 gccgggcacg agcgtcacga gcgcagcgta gttgcgcccg ttcagaggaa gttccgtcac
     2341 ctgcttttcg gagatgaggc tcgacacctt gccgctctca gtctcgacga ggcgcgccgc
     2401 gtccgtcacc gtcaccgtct ccgtcaccgc gccgacttcg agtttgagat cgacgaccag
     2461 actatcgccg acgtgtacct cgacgcccgt ggcgaccgtc tttttgaatc cttgcggcga
     2521 cgcgctcacg ctgtagcgtc cgatgggcaa actttgcgcc gagtagtcgc cgtcgtcgtc
     2581 cgtcttgacg gtgcgcgtct gtccggtcgt ctcttcggtg atgacgacct ccgttttggg
     2641 gacggcggct ccctgcgggt cgcgcacgct gccgctgatg ctgccgacgg tgctcgtctg
     2701 ggcatgaacg ccggtcagca gacctgctcc aagcaagacc actgcgaggt gaagagttaa
     2761 cttctttttc atggctctga ttctcctgac gccttccgga agtctctccg gcttgacgga
     2821 cggcacttga cgattaatca aacgaaacgc tcaagatgaa cccttgaaca gggttcgtga
     2881 agttttgcag cgtgaggcgc gtgccccggc tccctagccc ttctctggtt tcgctttcct
     2941 cgcagctacg aattaagagt tcaaaagtcc ggctgagcgc cgggtagatt aagacttaca
     3001 tacactttat acccgttgtt aaccatccca aaagttaccg tcagtaagat acggtataca
     3061 tgccggttca gggcgcatat ccctgagaaa acagcgcgtt acatgaaaga tgtaacgggg
     3121 ttacatggca ggcggcgcaa actgcccgca aggttggcct gtcgccgccg gtggcagttg
     3181 ttgaaaagcg gcgggtaggc ttataattcg gccaccgtca cacgggacgc gccagccttt
     3241 agtttggaca ccgaacaatc tgggttaaat tcgcgcttgc ttaagaacga cgcccctccg
     3301 cgtcgtctgc atctccgggc gtactcttaa atcaggttag tgatcgagat tatggaatta
     3361 ggcaaggaac agaaacttga gcagttggag aaagtgctgc atagccgcac gctccaaaac
     3421 tccgagagcc tgaaggcgtt cctccgcttc gtcgtggaga agacggttgc cgacgaggac
     3481 gtgctgctca aggagtacac gattgccacg gaagttttcg gacgcaacgg cgactacgac
     3541 ccgcgcattg attcggtcgt acgcgtgcag gccgggaggc tgcgtacgaa gctacaggag
     3601 tattacacca cggagggcaa ggccgatccc gtcatcatcg accttcccaa agggcactac
     3661 caccccgtct tcaactgccc ccacccgcca gccgcgattg agacggcgac cggcgtgacg
     3721 gcggatagtc aaacgacgac agaggcggcg cgcgcggcgg atgctcaagc gcaggccggg
     3781 agcgcgcacg cgcgtggccg gggcagagcc ttgactttcc tgctggccgc gctcgtcgtc
     3841 gtgctcgtgg ttgccgtcgc ggcgttgtac aactcgaacc gtgagttgcg gcggcaggcg
     3901 tcgaacgcgg acgccgcgct cgaccccgcc gacttcaagg ccgtctggag tccgttcatc
     3961 agcgatccgg aatcgccctt gctcgtcctg agcaacccga ccgtctatcg cttcctgaac
     4021 gaggccgacc ccgactcgct tgagcaacgt gcgatccagt tgacgcccga acaaacccgc
     4081 tcgcttgttg acgcgccgga gttcagagga caatggacgg gcggcgactc gccccggctc
     4141 atcccctcgc tcggcatgta cacggggatg ggcgaggcca tcggcctcta tcgtctgaca
     4201 gacctcttcc gcacggcgca gaagaccatc ctgctccggc aaagccgaca cgccagcgca
     4261 gccgatttga agtatcgcaa cgtgatcctg ctcggcagca tttacgtcaa cgaatggacg
     4321 cgccgcctgc ctacggtgga gagtttcaac tacacattct ccgcgacgat tgagaaccgc
     4381 gacccgcagg cgggcgagga gcgcgagtac aaaccgcagt tcaacccgca gacgggcgag
     4441 ttgacggttg actacgcgct catcaccgtt aagccgaacg tctccggcga acatgccgtg
     4501 atgacgctcg caggtatttt cagcgaaggg acggaggccg ccgccgagtt cgtcacgacg
     4561 cgcaaccatc tggccgtcct cggtcaacgc ctgcgccaac tcggcgggca gaacgcgccg
     4621 ccgaaatatt atcaagccct cctcaaggtc gaagtcgaga acggcacgcc gaccaccatc
     4681 acgctcctct ctctccgcgc gctgcctgaa aaacagtgac gagtgacgag caagcagccg
     4741 tcaacgtgaa gttgtcactt caaattccgg gcgcgcttct tacaactaca actcagtttt
     4801 tccttcgcgc ctgtgcgcct ctacgtgagt tatctccttc ccaaggataa gtctcacgca
     4861 aagacgcaca gatagcgcgg cgggggttgt gaagcgtgct ttgagtcgta tccgaccgca
     4921 agccgtttcg cctgtcactc gtcacttgtc acttgccact cgtcactaaa gtaaacttgt
     4981 cggagagtcc gccgacgagc acttcgaagt ctcccggctc gacgacgggc tgattgttct
     5041 gaccgatgaa ggagaggtct gcgcgttcaa gctcgaaggt gagcgtgcgc gtctggccgg
     5101 gcgcgagatg aactttggca aagcgtcgga gccgcttgtc gggcggcgtg atggtcgcca
     5161 cgagatcgcg cacgtaaagc tgcacgactt cgctgcccgc gcgccttccc gtattcgtca
     5221 acgtcacact gacgcccagc ttgtccgcct cgctcagact cttccggctc aggcgcaggt
     5281 cgccgtactt gaaagacgtg tagctcaggc cgtggccgaa tgcccattgc gggttgaagg
     5341 gcgtgaggcc gaaggtcgtg ctctcgttct cgtaagactt gtgatcgtag ttcaacagcg
     5401 cgttcgggtg gcgcgggtag gtgaagggca gacgccccga aggattgtag tcgccgaaga
     5461 gcacgtccgc gacggcatcg ccgccctcgt tgccggggtt gtaggccaac aggatcgcgc
     5521 ccgcgcgttc ggcgatgcgc gcgatgatgc gcgggcgacc ctctacgagc acgtagacga
     5581 cgggcttgcc tgcggcggcg acggcctccg cgagtcgcag ttgcggttcg ggcagagtga
     5641 gatcagtgat gttgcccggc gtctccgtgt aagagccttc gccgagacac aggatcacga
     5701 catctgcatc acgcgccgcg cgggctgcgg cggcgatgtc aacctcctcg tcgaagtccg
     5761 tgggcgtgac gtttgaaggc gcgttgccgt tgcgcctgat gcgcgtgccc gccacgtagc
     5821 tcatgtttgc cttgccgact ttcgcttcga ttgcctgccg gatcgtcggg cggtctttcg
     5881 ggtagagcga ctcctcggag ccttgccaaa cataggtcca accgttgttg agcgagatga
     5941 gcgaatcggc tgtgggacct gtcacgagca ccttgcggtt cttcgcgagc ggcaacagtt
     6001 cgttcgtgtt cttcaagagc gtcatcgctt cgcgcgccgc ttccagactc atgcggcgcg
     6061 actccggcgt ggcaaacttc gcacgcagcg acgcgtccgg catcgagtcc gtgaatagtc
     6121 cgagttcaaa tttcagagtc aggatgcgac gcacggcctc gtcaatgcgc gactgcggaa
     6181 ctgcgccctc acgcgcgagc gcgagcaggt gatcggcgaa gctgtagtcg ctcggcacca
     6241 tgctcatgtc aactccggcc atgacggcca tgcgcgtcgc ctctttttcg gtcgccgcga
     6301 cgcgatgaat gttgaccagc tttttaatgt cctcccagtc cgagacggcg acgccgcgaa
     6361 agccgagttc tttgcgcagg atgtccgtca gaatgaagtg gcttgcgtgg acgggcacgc
     6421 cgttgatctc gccggagttg atcatcacag tgcgcgcgcc cgcgtcaacg gcagccttga
     6481 agggcggcag cacatactcg cgcaggtaaa tctcaggaat ccaggcgggc gtccgatccc
     6541 gcccgctcag aggaaggccg tagcccatgt agtgcttgag acacgcggcg acgcgctcgg
     6601 acgaagagag gttcagcccc tcgtagccgc gcacggaggc gacgcccatc acagagatga
     6661 gatacgggtc ttcgccgtag gtttcccaca tgcgcggcca gagcggctgg cgtccggtgt
     6721 cgaggacggg cgagaaggtc caggggatgc ccgcggcgcg cgtctcgatg gccgccactg
     6781 ccgaagcgcg ctccatcagt tcgggattcc acgtcgcggc catggcgagc ggttgcggga
     6841 agagggttgc gccctgcacg tagtttgcgc cgtggatcgt gtcgatgccg tagaggactg
     6901 gaatcttgtg ccgcgtgcga cgggcggcct cctgaatctg acggatggtg tcgtgccaac
     6961 ggtcgagcgt gagcgcctga tctgcgacgt tcaggatcga gccgacgccg tacttgacga
     7021 ccgccttctc aagtttcgcc gggtcgatct tgatctcctg attcctgccc gtcgaaacca
     7081 tgctgatctc aagttgggtc atctgcccga ccttctcttc gagcgacatc ttcgacagca
     7141 gttcgtctgc acgccgcgcg gcctccgcgt tcgacgcggg cggggcgagc atccggcgaa
     7201 agacggtgtg gctgtcggtt agttcgctgg cggtcgctgt cgtagtggca gtgcgcgcgg
     7261 aggcggcggt ttcctgttgc cgtgcggcgc tcgtgtggag cggcagcagc gcatacagag
     7321 atagtgcggc gaaggcgtag gtcgcgaaca ttcttttcat gtcggaattt tcccttgctg
     7381 actctgagga tggactcggc gttacggcgc acttgtcgag cctgtatcgt gcggatgact
     7441 tctataatgc gggcggggtg aaacgtaagt tacaaaccgt taagcgggac tttgcagatt
     7501 gccgattgcg taatgcagtg tgtggttgag aaggttaact cacttctcac ctacgcccgg
     7561 ttcgcaatcg ttaatcctca cactattttt tgtgcgactt gttgaggcgt tcggctgtcc
     7621 gcctgatgcc gccgctgagc aggatgatgt ggcgcaggtc gcggttggcc ttcgcggact
     7681 gttcgacgtt gacgacttcc ggcactttga agacagggct ttcggtgaaa ctatcgatcc
     7741 gcttgcacat cgtcacgagc gcgtctttta actgctcatc gccgatctcg acgggcgttt
     7801 ccgttgcctt cttctcgttc gcggcaactt cgcgcaacag gtgcgtctgc atacgcccgg
     7861 cgcgacggtt gacctcaccc gtctccgccg cgatgaactt gtagtccagc ggcttctcgg
     7921 actgtaggtg gcgcacgagc ttgttgcgca gaatctgtat gttccgaaag tcttcacgca
     7981 actgttcggc ggcggcctgt gcgcccctcc ggtctttaga cttcaccgcc cccaacatct
     8041 ccgcgctacg gagttgcgcc tcgcgctgct gccgctcgcg cacctctccg aacgggtcgc
     8101 gctgccccgg aggcggcggg ccgcccggcg tagtctgctg cgcccgcgcc gaatgcgccg
     8161 cgagcgtcaa cagacagacg accgcgacaa agcccgccac gatgattgac gaagacgcac
     8221 gctgattctc catagcagtc tccttaaata tcttgcagag agtaggcggg cagtataatc
     8281 ccgcacctgc cccccgacaa tacctgcgag tcctatcaag tctttagcga ctaccccggc
     8341 cactttcggc cagcgtcatg cttgagcaga aacttatgtt gtcgaatcta tgaaattttt
     8401 tacaaaaacc tcttgcatta ttaaaacggc tatgtataat gtgcgcctca ctgcgggagt
     8461 ttactgcctg taacccattg tctgcctttg ccgaaagtta gtctcagtcg agtcggcatc
     8521 gggttcaaca tcaacgtaaa ttcataacct cgcgcaccct gcggagagtt acggattcct
     8581 caccgtagct atccttgcac gagtctctgt tatcggcgtc attatttaac aggagcctcg
     8641 tccggcggac tggttctatc tacctaaacc tcgcaacccc tccaaaggag tagaaatgaa
     8701 gagatttttt gcgtcgattc ttgttatcgc cctgtgctct atgatggttt cgccggggct
     8761 ggtttatagt cagcgttcga acaaggcggc caagctggcg cgcgccgaag cggccagcct
     8821 gctgcgcctg caaaagaaac ttgccagaaa aggcatcgcc cccaacgacg ttaagaccct
     8881 gcgcgttttc gaggacacag accgtgcgga acgcgtgcac acgcacgtcc agcagacaca
     8941 tcaaggcgtc cccgtcttcg gcggcgaagc gattgtccac ctcaatgccg cgaccgaaga
     9001 ggaagcacag ccgacgaccg acacactcat cgccggagtc tctgtttcca cgacgccggg
     9061 aatgacggtg ggcgacgccg tccgtctggc gaagtccgag tacaaagtga agaagggctg
     9121 cgacgaatgc ttcacggacg cgccgcagac tgatctgtgg gtgcttcgcc acgaaggcga
     9181 agatcacctc gtctaccgcg tgcaactctc gcgcctcgac ggcacggagc acacgtccga
     9241 gccggtctac ttcatagacg cacacacggg cgcgctcgtc tggagttacg acaacttgca
     9301 gacgcaggcc gcgaccggaa ccggcgtttc cctctacagc ggcacgcagg ccatcaacac
     9361 cttcaagtcc ggcacgagct actacatgga agacaacgcc cgcgcgctcg gcaccttcga
     9421 taaccgcaac tcggcgaaga tcaactcgat gctcggttac attacgagct acggctcgat
     9481 gtacaggttc gccgacgccg acaacttctg ggacgcggcg accgccagag cgggcgtcga
     9541 tgcacactac ggcgcggcca agacttacga ctacttcctc aacgtgcatg gtcgcaacgg
     9601 catcgacggc gcgggcggcc ctcgcacgct ggcttccgcc gacggttcaa ccggcctcat
     9661 cagttccatc gtccacttcg gcaaggccta caacaacgct ttttggagca gcaccaagaa
     9721 ccagatgttc tacggcgacg gcgacggcac gaacttttcg cctctcgtca cgctcgacat
     9781 caccggccac gagatgcagc acggcatcac ctcgcgcacg gcaaacctga cctacgcgaa
     9841 cgagtccggc gcgctcaacg aatcatggtc ggacgttttc ggcgcgatgg tcgaacttta
     9901 cgcagaggga caggagacgc ccactacttg gtggatcggt gaagacgcca acactcccgg
     9961 cgtcgcgggc gacgctctgc gctacatgga caccccgcac aaggccggca actacggcta
    10021 cacgacgaac gacgaccccg atcactactc ggagcgttac accggcacgg gcgacagcgg
    10081 cggcgtccac atcaactccg gcatccccaa tcacgtcttc tatcttttgg ctaaaggcgg
    10141 cacgcacgcg cacatcgtgg gtgctcccgt cacgggcatc ggcgctgaca aagcggccaa
    10201 aatctggtac aaggcgctca ccacctacat gacctcctcg accaacttca cgggcgcgcg
    10261 cacggcgacc ttggacgcag ccaacgccct ctacggcgtg ggcagccccg agtcaatttc
    10321 cgtcgcgcag gcgtggtctg cctgtgggat actctaggct cccggccaga gcgtaaagca
    10381 cagtcgaagt agagagggcg cggcgg
//
LOCUS       DASWNS010000009         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_415696, whole genome shotgun sequence.
ACCESSION   DASWNS010000009 DASWNS010000000
VERSION     DASWNS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415696"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VGB76_00180"
     CDS             <1..383
                     /locus_tag="VGB76_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097340.1"
                     /translation="DGIIATNTTTARVNLVHTPAGVASSIGAGGLSGAPLRACSTEVI
                     RALYRLSRGEIRIIGVGGIFNAAHAWEKICAGASLVQLYTGFIYEGLTAARDINEGLA
                     ELLRREGFRTLEEAVGCRAEEPVN"
     gene            499..1254
                     /gene="pyrF"
                     /locus_tag="VGB76_00185"
     CDS             499..1254
                     /gene="pyrF"
                     /locus_tag="VGB76_00185"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY0097341.1"
                     /translation="MHNKLIIALDVETATEARRLFSLLGAEAGMFKVGSQLFTAAGPE
                     FVREITGAGGRVFLDLKFHDIPNTVASACREAVRLGVSLFNVHAAGGGEMMRRAAEAT
                     RETAARENLPRPELIAVTVLTSADAGVLREIGVANELEAQVKMLAGLAASNGLDGVVA
                     SPHEIRAVRSVVARESFLLVTPGVRPAAAAHDDQRRVMTPGEAVRAGADYIVVGRAIL
                     NAPDPQRAAQEMVEEMQRAGRELSSIRSGEKSI"
     gene            1475..3076
                     /locus_tag="VGB76_00190"
     CDS             1475..3076
                     /locus_tag="VGB76_00190"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY0097342.1"
                     /translation="MHRRHRLLLPTFTLLFVISTLPVSLSAQQTSSNAPAAPAATGEF
                     SPAPYRAGEKLTYNVSFSNFLSAGYVELFNAGRGQFFNREGFELRARVQSVGIVRAAL
                     YAIDNEYTAYVDPQTGLPFRTRIQNLEGQGAAQGSTIAAQLPPPVEATPATYDFLSAL
                     YRMRALPLTPGATYRVSAQGGVMQYDAEVRVVGRETVKTPAGSFNAVVTQLRVRGNPA
                     ASDYRPRIYFTDDELHVPVLLTARLKTGELRAELASSELINLTPQAPTDAGGVIALQP
                     NTNPPPPGRVVVPSPAAGGDGAVAAGASDANSNLSDLPFKVGEQLNFNFFLGTSPQPV
                     GIASYHVRARNRYGGRDGLWLSATMGTTNALQQLFPVADKIETYVDVKTLLPFRSQLQ
                     IREGNHRLQGVVSLDQERGSATVTDGSTIEIPVGTYDLVSVIYALRSFDLTPPKRNAV
                     SLLINKRPRTLFITSLKRETIELGGKSIPAYQLALATDEPQGNRLNLRLWVGSDRRRL
                     PLRLTATTPLGPVRADLSIIPLTTQ"
     gene            3240..3551
                     /locus_tag="VGB76_00195"
     CDS             3240..3551
                     /locus_tag="VGB76_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEY0097343.1"
                     /translation="MVSVNNNFMDASATEGGRAAKNERRGALTLNALPYGQEARVVSV
                     EGEGAVARRLMEMGVVPGAPVCVIKAAPLGDPIEVRVRGYHLALRRAEAQTILVVTGD
                     R"
BASE COUNT          689 a         1138 c         1112 g          646 t
ORIGIN      
        1 ttgacggcat catcgcgacc aacacgacga cggcgcgcgt gaatctcgta cacaccccgg
       61 cgggcgttgc ttcttcaatc ggcgcgggcg gtttgagcgg cgcacccttg cgcgcgtgtt
      121 cgacggaagt gattcgtgca ttgtatcgtc tctcgcgcgg agagatcagg attatcggcg
      181 tcggcggaat cttcaacgcg gctcatgcgt gggagaagat ttgcgccgga gcgagcctcg
      241 tgcaactcta cacgggtttc atctacgaag gactcaccgc cgcacgcgac atcaacgaag
      301 gactggcgga acttctccgg cgcgaaggct ttcgcacgct cgaagaggcc gtcggctgcc
      361 gtgcggaaga acctgtcaac tgatagcgat tcgcagttag ctgtcagcag agagccggat
      421 ggttcggagt tatcgttgaa actgatagca ggctatcttg ttgccaacag ttaatagcta
      481 tcagctaaca gcctctttat gcacaacaaa ctgatcatcg cgctcgatgt ggagacggcg
      541 acggaagcgc gcaggctctt ttcattgctc ggcgcggagg cggggatgtt caaggtcggc
      601 tcgcaactgt tcaccgctgc gggaccagag ttcgtgcgcg agatcacggg cgcgggcggg
      661 cgcgtcttcc tcgatctcaa attccacgac atcccgaaca cggtcgcctc cgcctgccgc
      721 gaagctgtgc ggctcggcgt ctcgctcttc aacgtgcatg cggcgggcgg aggcgagatg
      781 atgcggcgcg cggcggaggc cacgagggaa accgccgcac gcgaaaactt gccgcgaccg
      841 gaattgatcg ccgtcaccgt gctgacgagc gcagacgcgg gcgtgctgcg ggagattggc
      901 gtcgcaaacg agttggaagc gcaggtgaag atgctggccg ggttggcagc gtcgaacggg
      961 ttggacggcg tggtcgcctc gccgcacgag ataagggcgg tgcgctcggt cgtcgcgcgc
     1021 gaaagtttcc tgctcgttac gcccggcgtg cgccctgcgg cggcggcgca tgatgatcag
     1081 agacgcgtga tgacgcccgg cgaagccgtg cgcgccgggg cggattacat cgtcgttggg
     1141 cgcgcgatcc tgaacgcgcc cgacccgcag cgcgcagcgc aggagatggt cgaagagatg
     1201 cagcgcgccg ggcgagaatt gtcgagcatc aggtcaggag agaaaagcat ctgaagtgag
     1261 gccgccggtg gcgcgacgcg cgccgttatg tcgtcgcctg acaggtgcgc ccgcgtagcc
     1321 gtgtagtgta agctagcgaa tatcgtttcc cccgtcgaag gtttaggaac agcaacggcg
     1381 cgtgcggcgt gtaaggctga atgctccgtg ctgcttgctg cgggcgggat gtaggaaaag
     1441 ttttagcgaa cctgcggaaa tgaggaagtt acttttgcac cgaagacacc gattgctgct
     1501 gccgacgttc acactcctgt tcgtaatctc gacgcttccg gtgtcgctct ccgcacagca
     1561 aacttcgagc aacgcccccg ccgccccggc agcgacgggc gaattctccc ccgcgccgta
     1621 ccgtgcgggc gaaaaactga cctataacgt ctccttctca aacttcctga gcgcaggcta
     1681 cgtggaatta ttcaacgccg ggcgcggcca gtttttcaat cgtgaaggct tcgaactgcg
     1741 tgcgcgcgtc cagagcgtcg gcatcgtgcg cgccgcgctc tatgccatcg acaacgaata
     1801 cacggcctac gtcgatccgc aaaccggact cccctttcgc acacgcatcc agaatctcga
     1861 aggacagggc gcggcgcaag gctcgaccat cgcggcacaa ctcccgcccc ccgtcgaagc
     1921 gacgcccgcg acttatgatt ttctctccgc tctctatcgc atgcgtgcgc tgccgctcac
     1981 gccgggcgca acctatcgcg tcagcgcaca gggcggcgtc atgcagtacg atgcggaggt
     2041 gcgcgtcgtc gggcgcgaga cggtgaaaac ccccgcgggc agtttcaacg ccgtcgtgac
     2101 gcagttgcgt gtgcgcggca atccggcggc gagcgactat cgcccgcgca tctatttcac
     2161 ggacgacgaa ctacacgtcc cggtcttgtt gactgcgaga ttgaagaccg gagagcttcg
     2221 cgcagagttg gcgagttccg agttgattaa cctgacgccg caagccccta ccgatgccgg
     2281 gggcgttatc gccttacagc ccaacacgaa tccgccgccg ccgggaagag tcgtcgtgcc
     2341 gtcgcccgcc gctggcggcg acggcgcagt tgccgccgga gcgtctgatg cgaattcgaa
     2401 tctctccgat ctgccgttca aagtcggcga gcagttaaac ttcaacttct ttctcggcac
     2461 gtcgccgcaa cccgtcggca tcgccagcta tcacgtgcgc gcacgcaacc gctacggcgg
     2521 gcgcgacggc ctctggctct ccgcgacgat gggcacgacc aacgccctac agcaactctt
     2581 ccccgtcgcc gacaagatcg agacttacgt tgacgtgaag acgctgctgc ccttccgcag
     2641 ccagttgcag atacgcgagg gcaaccatcg cttgcagggc gtcgtctcgc tcgatcagga
     2701 gcgcggttcg gccaccgtga cggacggcag caccatcgag attcccgtcg gcacctatga
     2761 tctcgtctcg gtcatctacg ccctgcgctc gttcgacctg acgccgccca agcgcaacgc
     2821 cgtctcgctg ctcatcaaca aacgaccgcg cacgctcttt atcacctcac tcaaacgcga
     2881 gacgattgaa ctcggcggca aaagcatccc cgcctatcaa ctcgcgctcg ccacagacga
     2941 accgcaaggc aaccgtctca acctgcgcct ctgggtcggc tcagaccgtc gccgcctccc
     3001 tctgcgcctg acggccacca cccccctcgg ccccgtccgc gcagacctct ccatcatccc
     3061 gctcaccacc cagtaagagt tcgccgcacg cgcgaacccg ccgacgggca gcctccctag
     3121 atgaaattga aaattgattt catttaatct atactcatcg cgcgattgaa gacagacggc
     3181 ttttagttat tcgactcccc aatcctgagg ccgcgcgcca tcgtcgaagc aagcgaaaaa
     3241 tggtttcagt caacaacaat ttcatggatg cgtcggcgac tgaggggggg cgtgccgcga
     3301 agaacgaacg gcgcggcgca ttgacgttga acgcgctgcc gtacgggcag gaagcgcgcg
     3361 tcgtgtctgt ggagggagag ggcgcggtgg cgcgtcgctt gatggagatg ggagttgtgc
     3421 ccggcgcgcc cgtgtgcgtt atcaaggccg cgccgctcgg cgaccccata gaagtgcgcg
     3481 tgcgcggcta tcatctcgcc ctgcgccgcg ccgaagcgca aacgatcctg gtagtgacgg
     3541 gtgacaggtg ataggtgaca agataaagcc gtgaatcgtg aatcg
//
LOCUS       DASWNS010000010         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_130328, whole genome shotgun sequence.
ACCESSION   DASWNS010000010 DASWNS010000000
VERSION     DASWNS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130328"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VGB76_00200"
     CDS             complement(<1..1110)
                     /locus_tag="VGB76_00200"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEY0097344.1"
                     /translation="MADKEQFTIGVEEEYQIVDRETRELRPRALRILPDAQEAVGDSV
                     TPELYLSQIEIGTNICATLTEVRDELTHLRREIITAAESDGHLIAAAGTHPFSHWEDQ
                     ELTPKRRYRGIAEEYEQLAREQLIFGCHVHIGINDREAAIHVMNRVRLWLAPLLALAA
                     NSPYWLGCDTGYASFRTAIWRRWPMSGTPQVFRSRAEYDALVEKLVATRSVSDGTKIY
                     WDVRPSARFETLEYRVTDVCLTIDEAVMIAGLVRALTRTAYEHLMRGEPLTEVRPEVL
                     RAAKWRAARYGLDGELIDAIEGRALPAADLIEKLLAHLRPALETDGNWEEVAALTRYT
                     LARGNGASRQRQVFARTENFEAVVDLLVAETAAGTT"
     gene            1138..>2197
                     /locus_tag="VGB76_00205"
     CDS             1138..>2197
                     /locus_tag="VGB76_00205"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0097345.1"
                     /translation="MPKRSGLILPLMLFLSVAFNAAAAQTGRTRTLSGTILTQQNERI
                     AGVSILVRSASGEQRVESDAEGNFRLAVPAETLSLRFFGKSLALLSRTIKPDEPSENL
                     EIRVAYIVPPVHESVVIEAAALDPTLDRRNATVYNHTLTLRDDQLFQTLSAGIDAGQH
                     EGGGKSLEIRRFGFNLDHGGVGGGLKVLVNNVQQNQATQGHGQGYLGQLKSLTPELVE
                     DVDILNGPFSAQYGDFSGLGVVHIRLRESLPDTLTLRLQGGSFDTFRTFLAYSPTLAR
                     ADAFLAYEGTRTNGAFQNALRYGRDNLTANYTRRLTDKRALGFKFNFGRNAFFSSGQI
                     PLDEVAAGRLDRFGFIDPD"
BASE COUNT          377 a          718 c          701 g          401 t
ORIGIN      
        1 agtcgtgcct gcggcggttt cggcgacgag gagatcgacg acggcttcga agttttccgt
       61 gcgggcgaag acctgtcgtt ggcgcgacgc gccgttgccc cgcgccagcg tgtagcgtgt
      121 gagggcggcc acctcttccc agttgccgtc cgtttcgagc gcgggtcgca ggtgcgcgag
      181 tagtttctcg atgaggtctg cggcgggcag cgcgcgtcct tcgatggcgt cgatgagttc
      241 accgtccagc ccgtagcgtg cggcgcgcca cttggcggcg cgaagcactt cggggcgcac
      301 ctccgtgagc ggttcgccgc gcatcaagtg ttcgtaggcg gtgcgcgtga gcgcgcgcac
      361 gagtccggcg atcatgacgg cctcgtcaat ggtcaggcaa acgtccgtga cgcgatactc
      421 aagcgtctcg aagcgcgccg aggggcgcac gtcccagtag attttcgtcc cgtcggagac
      481 gctcctcgtg gcgacgagct tttcgacgag cgcgtcgtat tcggcgcgcg agcggaagac
      541 ctgcggcgtg cccgacatcg gccagcgtcg ccagatcgcg gtgcgaaaac tggcgtagcc
      601 cgtgtcacag ccgagccagt agggcgagtt ggcggcgagc gcgaggagcg gcgcgagcca
      661 caggcgcacg cggttcatga cgtgaatcgc cgcctcgcgg tcgttgatgc cgatgtggac
      721 gtgacagccg aagatcaact gctcgcgcgc cagttgttca tactcttcgg cgatgccgcg
      781 atagcgacgc ttgggcgtca actcctgatc ttcccagtgg gagaaggggt gcgtgcccgc
      841 cgccgcgatg aggtggccgt cggattcagc agccgtgatg atctcgcggc gcaggtgcgt
      901 gagttcgtcg cgcacctccg tgagcgtcgc gcagatgttg gtgccgatct cgatctgcga
      961 gaggtagagt tccggcgtga cgctgtcgcc aacggcctcc tgcgcgtccg gcaggatacg
     1021 cagcgcgcgc gggcgcaact cgcgcgtctc ccggtcaacg atctgatatt cctcttcgac
     1081 cccgatggta aactgctctt tatccgccat gccttgaagt gtacaatagg tttcgtaatg
     1141 ccaaaacggt cgggcttgat tctcccactg atgctttttc tgtcggtcgc tttcaacgcc
     1201 gccgcagccc agacggggcg cacgcgcacg ctctccggta cgatcctcac acagcagaac
     1261 gaaagaatcg cgggcgtctc gatcctcgtg cgctccgctt cgggcgagca gagagttgag
     1321 agcgacgccg agggaaattt tcgcctcgcg gtgcccgccg aaactctgtc gctcaggttc
     1381 ttcggaaaga gcctcgcgct cttgtccagg actatcaaac cggacgagcc gagcgagaat
     1441 ctcgaaattc gtgtcgccta catcgtcccg cccgtacacg aatcggtcgt catcgaagcc
     1501 gccgcgctcg acccgacgct cgaccggcgc aacgccaccg tctacaacca cacgctcacc
     1561 ctgcgcgacg atcaactctt tcaaaccctg agcgcgggaa tcgacgcggg gcaacacgaa
     1621 ggcggcggca agtctctgga gatacgccgc ttcgggttca atctggatca cggcggcgtg
     1681 ggcggcggcc tcaaagttct ggtcaacaac gtgcagcaga atcaggcgac gcagggacac
     1741 gggcaaggct acctcgggca gttgaagagt ttgacgccgg aactcgtcga ggacgtggac
     1801 atcctgaacg ggccgttcag cgcgcagtac ggcgacttct ccggcctcgg cgtcgtgcac
     1861 atacgcctgc gcgagtctct gcccgacacg ctcaccctgc ggcttcaggg cggctcgttc
     1921 gacaccttcc gcacctttct cgcctacagc cccacgctcg cgcgcgccga cgcttttctc
     1981 gcctacgaag gcacgcgcac caatggcgcg ttccaaaacg ccctgcgcta cgggcgcgac
     2041 aacctgaccg ccaactacac gcgccgactc acggacaagc gcgccctcgg cttcaaattc
     2101 aacttcgggc gcaacgcgtt cttctcttcc ggccagatac ctctggacga agtggcggcg
     2161 gggcgtctgg atcgtttcgg cttcatcgac ccggaca
//
LOCUS       DASWNS010000011         4894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_415730, whole genome shotgun sequence.
ACCESSION   DASWNS010000011 DASWNS010000000
VERSION     DASWNS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415730"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="VGB76_00210"
     CDS             complement(<1..1366)
                     /locus_tag="VGB76_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY0097346.1"
                     /translation="MSAKNSSIAHPSKRVRAKLPPASNRRGYLRRFWLPAVLLLAISA
                     GGLTGIVVAYELNYSKAAGEVASLATYRPSQVTRVYADDGETVIGEFALERRIPLKYE
                     EIPPVLKNAILAVEDARFYDHVGIDPIRIIGAAWKNITADRREGGSTLTQQLAKNLFL
                     SREQTLTRKVNEWAIALQIERYYTKNQIMEMYVNHIFLGARAYGFEAAAQTYFNKHAK
                     DLTLDEAALLAGIPRSTAQYSPTVNPKAAKDRRDLVLELMARHSFVSQAEADAAKSKP
                     IKIADTAYYPPPSASHAWFEYPVEEIRQHIEEKYTTRVALGGLSVYSTINAAAQKKAY
                     EVVRHGLRVYDRGHRGWRSAYTNITTENNNGASQATPQQLASFVLPEWYGSNYAPDTY
                     ARGLVMSIDQARNVATVRFGSYTATLTAKDMGWSGRQPKSEFTVGDVAEFKIKEVDKD
                     ERRLT"
     gene            1605..1999
                     /gene="ssrA"
                     /locus_tag="VGB76_00215"
     tmRNA           1605..1999
                     /gene="ssrA"
                     /locus_tag="VGB76_00215"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2095..2973
                     /gene="murI"
                     /locus_tag="VGB76_00220"
     CDS             2095..2973
                     /gene="murI"
                     /locus_tag="VGB76_00220"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY0097347.1"
                     /translation="MNPNLPIGIFDSGVGGLTVYRALHEALPQERFVYLGDTARVPYG
                     TKSLATVERYALENARFLEARGIKLLVVACNTASALALPAIRASVGIPVIGVIEPGAA
                     AAVEASKGGRIGVIATEATVRSGAYSRAIRELAPEAEVLERACPLFVALAEEGWAESD
                     VARKVAAEYLTDLGQGRVDALVLGCTHYPILRRMIQETVGADVRLIDSGEATARSVEV
                     ALEREGLRRRKFDTATGQLLKERARERRLCDDLDHFYVTDAEERFARVAERFLGSAPT
                     RLEAVEVWGQDELHPA"
     gene            2954..3712
                     /gene="rph"
                     /locus_tag="VGB76_00225"
     CDS             2954..3712
                     /gene="rph"
                     /locus_tag="VGB76_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY0097348.1"
                     /translation="MSFTQLKTSYTRTDGRAAAALRAVSITPNFMPYAEGSALISMGN
                     TRIICTATLEESVPRFRRNSGQGWLTAEYAMLPRATTQRTQRETGRGGPSGRTHEIQR
                     LIGRSLRAVFDLALLGERTLMVDCDVLQADGGTRTASITGAYVACALACQKLQRAGKI
                     ARTPFKAEVAAVSVGIVEGTPLLDLKYDEDSRAAVDMNVVCTGDGRFIEVQGTAEGDP
                     FSRAEMDELLALASVGLEELFAAQRAALLPAPNA"
     gene            3863..4387
                     /locus_tag="VGB76_00230"
     CDS             3863..4387
                     /locus_tag="VGB76_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097349.1"
                     /translation="MFIFIAYGAFMLTAALPALQAQEEGAQKYTNERLEYALELPNAK
                     WRALTRPDSVHNHTEFIYGDRSDGLLRIRKEVVDAGTTATDLARRDHDLKFRFQPGYV
                     ESKEKEARFVGRINGVTSAYEYTGSGKAMIGRVYYLQADNRTIYVLHFTGARDKLPLL
                     RNQTDSIARSFHLK"
     gene            4494..>4894
                     /locus_tag="VGB76_00235"
     CDS             4494..>4894
                     /locus_tag="VGB76_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY0097350.1"
                     /translation="MKLANGLAVRPSRIDGSGCFATIHFRKGFKIAEYAGERISRREV
                     ARRARTRRKLRICAINSYWSLDGSRGGNGTHYINHSCAPNSYMRITHGHILFIALRDI
                     APGEEITLDYVTTLHSNDKRCHCKAPTCRGR"
BASE COUNT          973 a         1413 c         1544 g          964 t
ORIGIN      
        1 ctgtcaggcg gcgttcgtcc ttgtcaactt ccttgatctt gaactcggcc acgtcgccca
       61 ctgtaaactc gctcttcggt tgacgacccg accagcccat gtccttcgcc gtcaacgtcg
      121 ccgtgtagct gccgaagcgg acggtcgcta cgttccgcgc ctgatcaatc gacatcacga
      181 ggccgcgcgc gtaagtgtcg ggcgcgtagt tgctgccgta ccattcgggc aggacgaaag
      241 aggcgagttg ttgcggtgtg gcctggctcg cgccgttatt gttttccgta gtgatgttgg
      301 tgtaggccga acgccagccg cgatggcctc gatcatagac gcgcaagccg tgacgcacga
      361 cctcataagc cttcttctgc gccgccgcgt tgatcgtgga atagacggag agcccgccga
      421 gcgcgacgcg cgtggtgtac ttctcttcga tgtgctgcct gatctcttcc accggatatt
      481 cgaaccacgc atgcgaggcc gaaggcggcg ggtagtaggc agtgtcggcg atcttgatcg
      541 gcttcgactt ggctgcgtcg gcctcggcct gtgagacgaa actgtgtcga gccatgagtt
      601 cgagtacgag gtcgcgtcga tctttcgccg ctttggggtt gaccgtcgga gagtattgcg
      661 cggtcgagcg cgggatgccc gcgagcagtg cggcttcgtc gagcgtcaaa tccttggcgt
      721 gcttgttgaa gtaggtttgc gcggcggcct cgaatccgta ggcgcgtgcg ccgaggaaga
      781 tgtggttgac gtacatctcc atgatctggt tcttcgtgta gtaacgctca atctggagcg
      841 cgatcgccca ctcgttgacc ttgcgcgtga gcgtctgctc gcgcgagagg aagaggttct
      901 tggcgagttg ctgcgtcagg gtcgagccgc cttcgcgcct gtctgccgtg atgttcttcc
      961 acgccgcacc gatgatgcgt atggggtcga tgccgacgtg gtcgtagaag cgcgcgtcct
     1021 cgacggcgag gatcgcgttt tttaagacgg gcggaatctc ttcgtacttg agcgggatgc
     1081 gccgctccag tgcgaactcg ccgatgacgg tttcgccgtc gtccgcgtaa acgcgcgtta
     1141 cttgactcgg cctgtaggtc gccagcgacg ccacttcgcc cgccgccttc gaatagttca
     1201 actcgtaggc gacgacgatg ccggtcaagc ctccggcgga gattgccagg aggagaactg
     1261 cgggcagcca gaagcggcgc aggtagccac ggcgattcga cgccggaggc agtttcgcgc
     1321 gcacacgttt cgacggatgc gcgatactcg aatttttagc ggacatgtaa gatgatcctc
     1381 gttgccggac gacacgcgaa aaaaaatagc cgaaagcgcg tgtcgaaaaa caatcaagtc
     1441 aaaagtcagg agagactaac cggacgcaat tataacgcaa cctttgcctc tacgcgaacg
     1501 tctaaaagca gtgacaaacg atgagtgaca ggtgacaagt taaaacagaa atcctgttct
     1561 ttaacctgcc acctgtcact gtgttataat cactctgcac gtttgggggc gaaaggcttt
     1621 cgacaggggt tttggtcacc gagcggatgc atgccgggct tactctttgc agctcgttaa
     1681 caagcactga gaaaaaacaa ttgccaataa caatgagttg gctcttgctg cctaattaaa
     1741 gttagccagt aagcgtctcg ccgcaagttg ctcaagcgcg cggagcgaga tgtcacttag
     1801 ttgagctggc ctgctctctg tgctcaggga gagcgggcga gataaaactg ggctagccgc
     1861 aggcggggtc ttcgtcgctt cactgacccg ctcgcagcga acgcgccgga gcaagaccgg
     1921 caacagagaa gcgtctaagc atgtagattc cgacggtgtc ggcctttgga cccgagttcg
     1981 attctcggcg cctccactta caaagtttta ccgcacgacg aaagcggcca gcagggataa
     2041 atggtgtgat accattcgcc ccggctggtc gttttcgcgt ctggtgaaaa tagattgaat
     2101 cctaatttac ccatcggaat atttgatagc ggcgtcggcg gcttgacggt ttatcgcgcg
     2161 ctgcacgagg ctttgccgca ggagcgtttc gtttatttgg gcgacacggc gcgcgtgcct
     2221 tacgggacga agtctctggc gaccgtggag cgttatgcgc tggagaacgc gcgttttctc
     2281 gaagcgcgcg gcatcaaatt gctggtcgtg gcgtgcaaca cggcgtccgc gctggcgttg
     2341 cccgcgattc gtgcgagcgt gggcatcccc gtcatcggcg tgatcgagcc gggggcggcg
     2401 gcggcggtag aggcttcgaa gggcggacgc atcggcgtga tcgcgacgga ggccaccgtg
     2461 cgcagcggcg cgtacagtcg cgctatcagg gaactcgcgc cggaggcgga ggtcttggag
     2521 cgcgcgtgtc ctctgttcgt cgcgttggcc gaagagggtt gggcggagtc ggacgtggcg
     2581 cgaaaggtgg ccgccgaata tctgacggat ttgggacagg ggcgcgttga tgcgctcgtg
     2641 ctcgggtgca cgcattatcc gattttgcga cgtatgattc aggagacggt cggcgcagat
     2701 gtccgcctga tcgattcggg cgaggcgacg gcgcggagcg tggaagtcgc gcttgagcgc
     2761 gaaggactgc ggcgtcgcaa gttcgacacg gcgacggggc agttgctcaa agagcgtgcg
     2821 cgtgaacgtc gtttgtgcga tgatctcgac cacttttacg tgacggatgc ggaggaacgt
     2881 ttcgcccgcg tcgcagagag atttttgggc agcgcgccga cgcgccttga ggccgtggag
     2941 gtttggggac aagatgagct tcacccagct taaaacgagt tacacgcgaa ccgacgggcg
     3001 cgccgccgca gctctgcgcg ctgtgagcat cacgccgaat tttatgcctt acgcagaagg
     3061 ctccgcgctc atctcgatgg gcaatacgcg catcatctgc acggcgacgc tggaagagag
     3121 cgtcccgcgt ttccggcgca acagcgggca gggttggctg acggcggagt atgcgatgct
     3181 gccgcgcgcc acgacccagc gcacgcagcg cgagacgggg cgcggcggtc cttccggtcg
     3241 cacacacgaa attcagagac tcatcgggcg aagtttgcgc gccgtcttcg acctcgcgct
     3301 gctcggcgaa cgcacgctca tggttgattg cgatgtgctg caagcggacg gcggcacgcg
     3361 caccgcttcg atcacgggcg catacgtcgc atgcgcgctg gcctgccaga agttgcagcg
     3421 cgcgggtaag atcgcgcgca cgccgttcaa ggccgaggtg gcggctgtga gcgtcgggat
     3481 cgtcgaaggg acgcctcttc tcgatctcaa atacgacgaa gactcgcgtg ccgccgttga
     3541 catgaacgtc gtctgcacgg gcgacgggcg tttcatcgaa gtgcagggca cggctgaggg
     3601 cgaccccttc tcgcgcgccg agatggacga gttgctggcc ttggcgagcg taggtctgga
     3661 agaacttttc gccgcacaga gagccgcgct cctccccgcg cccaacgctt gaagcgcacg
     3721 cgggcggggc aacacggcgc gagttcgcac gcatctgaca gggagcggct cgaaacactt
     3781 aataacactt aaccgggtag atcacacaaa gaacacgcgc gacaaaatca gatcaggagt
     3841 aaagttttaa attatgcgac gcttgtttat tttcattgcc tacggcgcgt tcatgctgac
     3901 ggcggcgttg cccgccttgc aggcgcagga agagggggcg cagaagtaca ccaacgagcg
     3961 gctcgaatac gcgctcgaac tgcccaacgc gaagtggcgc gcgctgacac ggcctgacag
     4021 cgtacacaat cacacggagt ttatttacgg cgaccgcagc gacggactct tgcgtatccg
     4081 caaggaagta gttgacgccg ggacgaccgc cacagacctc gcgcgacgcg atcacgatct
     4141 caagtttcgt ttccagccgg gctacgtcga aagtaaagaa aaagaggcac ggtttgtcgg
     4201 gcgcatcaac ggcgtcacct ccgcttacga atacacgggc agcggcaaag cgatgatcgg
     4261 gcgcgtctat tacctgcaag ccgacaatcg caccatctac gtcctgcact tcaccggagc
     4321 gcgcgacaaa ctcccgctcc tgcgcaatca aaccgactcg atagcgcgca gttttcatct
     4381 caaatagcgg ttagcgatta gctgttagct cttaaccgaa cgaagaagcg caacgagctt
     4441 ccggctaatc actaacgcct gatcgctaac ggttactccc tgacagcttc cctatgaaac
     4501 ttgccaatgg cctcgccgtg aggccgtcgc ggattgacgg tagcgggtgc ttcgcgacga
     4561 ttcactttcg caaaggtttc aagatcgctg agtatgcggg cgagcggatt tcgcggcgag
     4621 aggtggcgcg gcgcgcacgc acccggcgca aactgcgcat ctgcgccatc aattcttact
     4681 ggagtctgga cggcagccgg ggcggcaacg gcacacacta catcaaccat tcctgtgcgc
     4741 ccaactccta catgcgcatc acgcacggcc acatcctctt catcgccctg cgcgacatcg
     4801 cacccggcga agagatcacg ctcgactacg tcaccaccct ccattccaac gacaagcgtt
     4861 gccactgcaa agcccccacc tgccggggaa ggat
//
LOCUS       DASWNS010000012         6749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_415746, whole genome shotgun sequence.
ACCESSION   DASWNS010000012 DASWNS010000000
VERSION     DASWNS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415746"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VGB76_00240"
     CDS             <1..375
                     /locus_tag="VGB76_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0097351.1"
                     /translation="AGLSVGQKQLISFARALAFNPALLILDEATSSIDTETEQLIQQA
                     IERVMRDRTSILVAHRLSTIQRADRIIVLHHGEIREQGSHQELLTGRGLYWRLYKLQY
                     ADKSRDEAVPDLPLRDTLQYGD"
     gene            complement(503..1900)
                     /locus_tag="VGB76_00245"
     CDS             complement(503..1900)
                     /locus_tag="VGB76_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097352.1"
                     /translation="MKTVAVSSLLMFAFVSANAQQATQSPTPGQFVRPRIVASAPAQE
                     PQQRQTVQPPAPNASAPVKTVTPTPQTPPRISPVAPNVSNAPAQTPTVAAPPLTTPVV
                     VPSIPLKPATPISPNKVRARILESQRLFKTRITPTALGSSPNLNFVTLAVLDDDSSQI
                     HLLTLAKEVFLKKDNEMTVASSLGGFLRVRIVRSNYVNTAVTVSDLTGRQLAPLLVEY
                     PIEKFGKYRETAYYTSAHPALLTPELIKNGQAYVRTMVDLAAKRLKDKGHHMTPEVLD
                     MAERLCLVEHVDHERFRKESRLAVFNEVYALYALNELDTYRYSVSTAGAGGMVQMIPW
                     TYQMLRQRYPAIGLNPDFVSGMRNHGNALEAMLLYIRDTWSDLLLNETIAGALSNGIA
                     TPADLIAAGYNSNPAKLAGYIRRGGAAWRTLIPRETQMYLQIYQSLESLSPIKARAKQ
                     WEKAPAATTAQTPIS"
     gene            complement(2047..3504)
                     /locus_tag="VGB76_00250"
     CDS             complement(2047..3504)
                     /locus_tag="VGB76_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097353.1"
                     /translation="MLFVESKSLDQPVDGATRTSLLENLQEQGFVPLIRSARLHLYEV
                     ALAGAEQTRELERALAARADVEAEAPYVRGDIFCVEDYALFLLFGEAGDEEAVRAGII
                     YESETHEPLERLDAFCRSIGEALDASRRSGVSGGNGAGSGSGVAATLNGGATPVSEWK
                     RREPRVPDSFRRFAAAETATPANARGEMIEGWLRTSGMLEDTQARGFLRRLSEAHREG
                     RTASVLQASGDESVPAALLNRLAEVGLLKREILVSCRRDERALFRLPSPDAFSVLSGS
                     NAVCNECGAAIADERAEEVIVPTSLTTTLLQDGAWLATHLRSIIYTLGVPEKQIVARP
                     TGSDGEVRLMANVCGEAFLCLLHEGDWTTAQARRALDEQAKSDSAHLVLIATGKIQED
                     ARQRLREHARRRTQGGRELELILIEGLDSVAAELQPVFERVASNALAEELWELDTSLG
                     VSAGQLIATRFRLLRRSGEPRDLAASAVASIKSQV"
     gene            3866..4492
                     /locus_tag="VGB76_00255"
     CDS             3866..4492
                     /locus_tag="VGB76_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY0097354.1"
                     /translation="MSVKTLFPRALAASFALCLFASGVAAQETDQPRPRQVTQATQQD
                     GSNRLETDVFVVSEATPASVNRYETELAKPRLIAPRLFQFNQMMLAAIDTRIGAPYVY
                     GSSGPRVFDCSGFVWSVFQSAGVNFERGSARGFWTNFYPVSEDEKYKFGTLVFFNNLK
                     HVGIVADEKGFYHASTSQGVTYSPFNEYWTSRITGFRRVPLPAQTLAD"
     gene            complement(4620..4844)
                     /locus_tag="VGB76_00260"
     CDS             complement(4620..4844)
                     /locus_tag="VGB76_00260"
                     /inference="COORDINATES: protein motif:HMM:NF041655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MM0924 family protein"
                     /protein_id="HEY0097355.1"
                     /translation="MQDFLAKMMGQRVDIFCGGASSLHGEVVKVEGGVLHLKDAEGTR
                     CYVAVEKIIAVWERREENEHRAGFLSSVVK"
     gene            complement(4935..5411)
                     /locus_tag="VGB76_00265"
     CDS             complement(4935..5411)
                     /locus_tag="VGB76_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY0097356.1"
                     /translation="MGKKITDEQPRVYFDCSKCPAYCCALYERIQVTKRDLNRLAKHF
                     GLTVERTAKRYTKMWNGKERVLRRTPDPILGEACQFLDHETRGCSIYHARPEVCRTYP
                     DRSRCAYYDVLQFERRQQDDPNVLPLFQITFREVKKKVVTDEHGSEKIWEWKQEKK"
     gene            complement(5520..6656)
                     /gene="speY"
                     /locus_tag="VGB76_00270"
     CDS             complement(5520..6656)
                     /gene="speY"
                     /locus_tag="VGB76_00270"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEY0097357.1"
                     /translation="MAKKKSKFLRGRRIDPKPITGEVALAELVDETFLAYNAGRLREA
                     CQLFTQKMLEPDVTVGVSITGALTPAGLGISAIIPLMRAGFIDWIITTGANLYHDTHF
                     GIGLSLHQGNTSTSDITLRDEEVVRIYDIFFDYSVLLDTDEFFRRIIEGEEFQKAMST
                     AEFHHLCGRYVRERERKLGLEYKSLLAAAYEFGVPLYTSSPGDSSIGMNVAAKALQGN
                     KLAFDPSLDVNETAAIVLAAKRGAIHGKGDRGRKHGGKSAVFILGGGSPKNFMLQTEP
                     QIQEVLGIDERGHDFFLQVTDARPDTGGLSGATPGEAVSWGKVDPDRLPDAVVCYVDS
                     TIALPVLTAYALSRHEPRALKRLYERRGELMNLLLSEYEKSERR"
BASE COUNT         1313 a         2109 c         1926 g         1401 t
ORIGIN      
        1 gcgggattgt ccgtcgggca aaagcaactg atcagtttcg cgcgtgcgct ggccttcaat
       61 cccgcgctcc tgattctcga cgaagcgacg agttcgattg acaccgaaac cgagcaactc
      121 atccagcagg ccatcgaacg cgtgatgcgc gaccgcactt ccattctcgt cgcgcaccgc
      181 ctctccacca tccaacgcgc cgaccgcatc atcgtccttc atcacggcga gattcgcgaa
      241 caaggctccc atcaggaatt gctcaccggg cgcggcctct actggcgtct ctacaaactt
      301 caatacgcag acaagtcgcg cgacgaagcc gtgcctgact tgcctctgcg cgacactctc
      361 caatacggcg attgagcaag cggaaaactc taaagaagaa cggcgagcgg taattatctt
      421 cccgctcgcc gttcttcttc ttcctgcgct ttccgccctg cccttacggc ttcgccgcag
      481 ccgcgcgcac gcggatcaac ttttaactta tgggggtttg cgccgtcgtc gccgcagggg
      541 ctttctccca ctgcttcgca cgcgccttga tgggcgatag ggattcgagc gattggtaaa
      601 tctgtagata catctgcgtc tcgcgcggga tgagcgtgcg ccatgccgcg ccgccgcgcc
      661 gtatgtatcc ggcgagtttg gccggattgg aattgtaacc ggcggcgatc aggtcggcgg
      721 gcgtggcgat gccgttcgag agagcaccgg cgatggtttc gttaaggagc aggtcgctcc
      781 aggtgtcccg tatgtaaagc agcatcgctt cgagcgcgtt gccgtgattg cgcatgccgg
      841 agacgaagtc cgggttgagg ccgatggccg ggtaacgctg ccggagcatc tggtaagtcc
      901 agggaatcat ctgcaccata ccgcccgccc cggcggtgct caccgagtag cgataggtgt
      961 cgagttcgtt cagcgcgtag agcgcataga cctcgttgaa gaccgcgagg cgcgactcct
     1021 tgcggaaccg ctcatgatcg acgtgctcga cgagacagag acgctcggcc atgtccagca
     1081 cttcgggcgt catgtgatgc cctttatctt tcagacgctt ggcggccaga tcaaccatcg
     1141 tgcgcacata ggcttggccg ttcttgatga gttcgggcgt gagcagcgcg ggatgcgccg
     1201 aggtgtaata agccgtctcc cggtacttgc caaacttctc aatcggatat tcgacgagca
     1261 gcggcgcgag ttgccgcccc gtcaaatcgg agacggtgac cgccgtgttg acgtagttgg
     1321 aacgcacgat gcgcacgcgc agaaaaccac cgagcgaaga ggccacggtc atctcgttat
     1381 cttttttgag aaacacttcc ttcgccagcg tcaggagatg aatctgcgac gaatcatcgt
     1441 cgagtacggc gagcgtgacg aagtttaagt tgggcgagct gccgagcgcg gtgggcgtga
     1501 tgcgtgtctt gaagaggcgt tgcgattcga ggatgcgtgc gcgcactttg ttcgggctga
     1561 tcggcgtcgc gggcttcaac ggaatcgaag gcacgacgac gggcgttgtg agcggcggtg
     1621 cggcgacagt cggagtttgc gccggggcgt tcgagacgtt gggcgcgacg ggggagatgc
     1681 gcgggggcgt ctgcggcgtc ggcgtaacgg tcttgaccgg agcggacgca ttcggcgcgg
     1741 gcggctgcac agtttgacgc tgctgcggtt cctgcgcggg cgcgctggcg acgatgcgcg
     1801 ggcggacaaa ctggccgggc gtcggagact gtgttgcttg ctgcgcgttc gccgagacga
     1861 acgcaaacat taagagggac gagacggcaa ctgttttcat ctttctgtaa aacccttcgg
     1921 atggcaatag gttctgcggg gagcagaccc agctaagatt cgcttgcgag gcgcacggtt
     1981 gtcaagaaaa gttccaaaga ccaaatctcg aatcacaact cggaggcgtt ttccagactc
     2041 cggactttag acctgagact ttatggaagc gacggctgaa gccgcgagat cgcgcggctc
     2101 gcccgaacgt cggagcaagc gaaagcgcgt ggcgattaat tgtcccgcgc tcacgccgag
     2161 gctcgtgtcg agttcccata actcctccgc aagcgcattc gaggcgacgc gttcgaaaac
     2221 gggttgaagt tcggcggcga ccgagtccag accttcgatg agaatgagtt cgagttcgcg
     2281 cccgccctgc gtgcgccgtc gcgcgtgttc gcgcaggcgt tggcgcgcgt cttcctgtat
     2341 cttgcccgtc gcaatcagca cgagatgcgc gctgtcgctc ttggcctgtt cgtcgagcgc
     2401 acggcgcgcc tgcgcggtcg tccagtcgcc ttcgtgcagg aggcagagaa aggcttcgcc
     2461 gcagacgttt gccatcaggc gcacctcgcc gtcgctcccg gtgggacgcg cgacgatctg
     2521 tttttcgggg acgccgagcg tgtagatgat cgaacgcagg tgcgtagcga gccacgcgcc
     2581 gtcttgcagg agcgtcgtgg tgagagaggt cggcacgatc acctcttccg cgcgctcgtc
     2641 tgcgatggcc gcaccgcatt cattgcagac ggcgttgctc ccgctcagga cagagaacgc
     2701 gtccggcgac ggcagacgaa acagtgcgcg ctcgtctctg cggcaactca ccagaatctc
     2761 gcgtttcaac agacccactt ccgccagccg gttgagcaac gcggcgggca cgctctcgtc
     2821 gccagacgct tgcagcacgc tcgccgtgcg cccttccctg tgtgcctcgc tcagacggcg
     2881 caggaagccg cgcgcctgcg tatcctccag catcccggag gtgcgcagcc atccttcaat
     2941 catctcgccg cgcgcattgg cgggcgtggc cgtctccgcc gccgcgaagc gtcggaaact
     3001 atcgggcacg cgcggttcgc gccgcttcca ttcggacacg ggcgtcgcgc cgccgttcaa
     3061 cgtcgccgca actccgctgc cgctccccgc gccattgccg ccgctcaccc cgctacgacg
     3121 cgacgcgtcc agagcctcgc cgatgctgcg acaaaaggcg tcaagtctct ctaaaggttc
     3181 gtgcgtctcg ctttcgtaga tgattccggc gcgcacggcc tcttcgtcgc ccgcctcgcc
     3241 gaacagaaga aagagcgcgt agtcttcgac gcagaagatg tcgccgcgca cgtaaggcgc
     3301 ttcggcctcc acgtccgcac gcgccgccag agcgcgctcc aactcgcgcg tctgctccgc
     3361 gcccgccagc gcaacttcgt acaaatgcag ccgcgccgaa cgaatcaacg gcacgaagcc
     3421 ctgctcctgc aaattctcca acagagaggt gcgcgttgcg ccgtcaacgg gttgatccag
     3481 acttttgctc tcgacgaata gcatagtttg cgattatctc ctcctcaagg ccgccgcatg
     3541 ctatcacgaa cgtgcaaaaa attgtccatc ttcttcgcgg tttatcaccc aaaaattctt
     3601 tctttcgaac aattagcgta tgaaggcgtc acacatcgtt caagttgaat caatttccgc
     3661 gcccgtttgt ggttagtttc aggtcacctc gcgttttgcg tccgtgccct gtcacgaaac
     3721 agtcacattg gctcttgttt caatcatgtt acgactgtaa gatgttttgg ctgcacaaaa
     3781 tgcagcgcac tagccttcta acccctagaa gtatcccaaa ggcaattgtg gattttgaat
     3841 aaggagctac ggaagccccc gatctatgtc tgttaaaact ctgttccccc gcgcgctggc
     3901 cgcgtctttc gctctctgtc tgttcgcctc cggcgtcgcc gcacaggaaa ccgaccaacc
     3961 ccgcccgcgt caggtcacgc aagccactca acaggacggc tcgaatcgtt tggagacgga
     4021 cgtgttcgtc gtctcggaag ccacacctgc gagcgtgaac cgatacgaga ctgaactcgc
     4081 caagccccga ctcatcgccc cgcgcctttt tcaattcaac cagatgatgt tggccgccat
     4141 tgatacgcgc atcggcgcgc cttacgtcta cggcagttcg gggccgcgcg tcttcgattg
     4201 ttcgggtttc gtgtggagcg tctttcaatc ggcgggcgtc aacttcgagc gcggcagcgc
     4261 gcgtggtttc tggacgaact tctacccggt cagcgaggat gagaagtaca aattcggcac
     4321 gctcgtcttc ttcaacaatc tcaagcacgt cggcatcgtc gccgacgaga agggcttcta
     4381 tcacgcctcg accagtcagg gcgtcaccta ttcgcccttc aacgaatact ggacgagccg
     4441 catcacgggc tttcgccgcg tccccctccc cgctcagact ctcgcagatt aactctaagg
     4501 ttgctcctta gaacgacagg ccgacgttgt gcaactccca caacgccggc ctgttaactt
     4561 tttacgcgcc ctcgcacgat aggacgcaca ccccaacccg tgtgcgcgac tcccgcgcgt
     4621 tacttcacta cgctactgag gaagcccgcg cggtgctcgt tctcctcgcg ccgctcccac
     4681 acggctatga tcttttccac cgccacatag caacgcgtgc cctccgcatc tttcaagtgc
     4741 agcacgccgc cttcgacctt caccacttcg ccgtgcaagc tcgacgcccc gccgcaaaat
     4801 atatccaccc gctgacccat catcttcgcc agaaaatcct gcatcttcaa cctcccgaat
     4861 tcagtgacaa gtgacaggtg acaagttaaa gccaaagctt ttgtattaac ctgtcactcg
     4921 tcacctgtcg ctcgtcactt tttctcttgt ttccattccc agattttttc gcttccgtgc
     4981 tcgtccgtga ccactttctt tttcacttcg cggaaagtga tctggaaaag gggcaggacg
     5041 ttcggatcgt cttgttgtcg ccgctcgaat tgcagcacgt cgtagtaagc gcagcgcgag
     5101 cggtctggat acgtgcggca aacctcgggg cgggcatggt agatcgaaca gccgcgcgtc
     5161 tcgtggtcga gaaactggca ggcttcgccg aggatcggat cgggcgtgcg cctgagcaca
     5221 cgctctttcc cgttccacat ctttgtgtaa cgcttcgccg tgcgctccac cgtcaggccg
     5281 aaatgtttcg ccagccggtt cagatcgcgc ttcgtcacct gaatccgctc gtacaacgcg
     5341 cagcagtaag ccgggcattt cgaacaatcg aaataaactc gcggctgctc gtcggtaatt
     5401 ttcttcccca tgccattaaa agtgacgagt gacaagtgac aagtgacaag ttggagacaa
     5461 gaaaacaggt tctctgattt aacttgtcac tcgtcacttg tcactcgtca ctgtactcgt
     5521 caccgccttt cagatttctc atattcggat aacagtaaat tcatcaactc gccgcgccgt
     5581 tcgtagagac gcttcagcgc gcgcggctcg tggcgtgaca gcgcataggc cgttaagacg
     5641 ggcagcgcga tggtcgaatc aacataacac acaacggcgt cgggcaaacg atccgggtca
     5701 acctttcccc aggatacggc ttcgccgggg gtcgcaccgg agagtccgcc tgtgtcgggg
     5761 cgcgcgtccg tgacctgcaa aaagaaatca tggccgcgct cgtcaatgcc gagcacctct
     5821 tgaatctgcg gctcggtttg cagcatgaaa ttcttgggcg aaccgccgcc gagaataaag
     5881 accgcgctct tgccgccgtg cttgcgccca cggtcgccct tgccgtgaat cgcgccgcgc
     5941 ttggccgcga ggactatcgc ggcagtctcg ttcacgtcga gcgacggatc gaaagcgagt
     6001 ttatttccct gcaaagcctt cgccgcgacg ttcatgccga ttgaagaatc gccgggcgaa
     6061 gaggtgtaga gcggcacgcc gaactcgtag gcggcagcga gcagagattt atattcgagt
     6121 ccgagcttgc gctcgcgctc gcgcacgtag cgcccgcaga gatgatgaaa ctccgccgtg
     6181 gacatcgcct tctgaaactc ttcgccttcg atgatgcgcc ggaagaactc gtccgtgtcg
     6241 agcagcaccg aataatcgaa gaagatgtcg tagattctca ccacctcttc atcgcgcagg
     6301 gtgatatccg aagtgctcgt gttgccctga tgcagagaga ggccgatgcc gaaatgcgtg
     6361 tcgtgataaa ggttcgcgcc cgtcgtgatg atccaatcaa tgaacccggc gcgcatcaag
     6421 ggaatgatcg cggagatgcc gagtccggca ggcgtgagcg cgcccgtaat cgagacgccg
     6481 accgtcacgt ccggctccaa catcttctgc gtgaacaact ggcacgcctc gcgcagacgc
     6541 cccgcgttgt aagccagaaa cgtttcgtca accagctctg cgagcgcgac ctcgcccgtg
     6601 atgggcttcg ggtcaatccg ccgcccgcgc agaaacttac tcttcttttt cgccatgccg
     6661 acaatataaa cgaagaacgc ggaacgatga aatctagagc gactcttatt tcatcatccc
     6721 gcactcgggg ttcacaaact ctcttttga
//
LOCUS       DASWNS010000013         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_104191, whole genome shotgun sequence.
ACCESSION   DASWNS010000013 DASWNS010000000
VERSION     DASWNS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104191"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VGB76_00275"
     CDS             complement(<1..346)
                     /locus_tag="VGB76_00275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0097358.1"
                     /translation="MDEFLQTNRSLWNGWTRLHKHSRFYDIEGFKAGNSSLTPLELGE
                     VGDVEGKSLLHLQCHFGMDTLSWARRGAQATGVDFSVEAVELARSLAAELDLPAQFVC
                     SNVYDLPGALEGR"
     gene            complement(355..1221)
                     /locus_tag="VGB76_00280"
     CDS             complement(355..1221)
                     /locus_tag="VGB76_00280"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEY0097359.1"
                     /translation="MSKNYGENAPLSYNKYLRVADLIALQECLSDPAHHDELLFITIH
                     QTYELWFKQILHEIDASVALMNEDRAFVAARALTRVVEIEKVLVNQIHLLETMTPINF
                     LGFRDELNPASGFQSMQFREIEFASGLKAQGTLDNFREDEFAHTRLQARFDAPTLADA
                     FYAYLRRRGLDAPADDATLNKEERTRRYGQRTRAVLELLTHFETRPEEFGLAEALIAH
                     DEYFSLWRSHHVKMVERMVGTKRGTGGSEGVGYLRTTLEQRFFPELWEARTYLDAKHG
                     AGGCPFAGHQQQ"
     gene            complement(1320..>1827)
                     /locus_tag="VGB76_00285"
     CDS             complement(1320..>1827)
                     /locus_tag="VGB76_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0097360.1"
                     /translation="HPRLHATLEPKVTGWMAHEHPFAFEGRMTYAPGIARFLHGSPSI
                     PALYAARAGYSIINEIGVAQIRAKSTRQTTRLIEIADAAGFNVTSPREADRRGGTVTV
                     NVEHAAAVTRELIRREIIVDFRPGAGIRISPHFYTKDEELDIVIREMRRIIDTRAYEA
                     HEATGAAY"
BASE COUNT          325 a          503 c          620 g          379 t
ORIGIN      
        1 atcggccttc gagcgcgccc ggcagatcgt agacgttcga gcagacgaac tgcgcgggaa
       61 gatccaactc ggcggcgagc gagcgcgcta actcgaccgc ctcgacggag aagtcaacgc
      121 ccgtcgcctg cgcgccgcga cgcgcccacg agagcgtgtc catgccgaag tggcattgca
      181 ggtggagcag cgacttgcct tctacgtcgc cgacctctcc gagttccaga ggcgtcagag
      241 aagagttgcc cgccttgaaa ccttcgatgt cgtagaagcg cgaatgcttg tgcagccgtg
      301 tccagccgtt ccagagcgag cggttagtct gcaaaaattc gtccatgaca actgttactg
      361 ctgctggtgt ccggcgaagg ggcaaccgcc cgcgccgtgt ttcgcgtcga ggtaggtgcg
      421 cgcttcccag agttcgggga agaaacgttg ttcgagcgtg gtgcgcaggt agccgacgcc
      481 ttccgagccg cccgtcccgc gcttcgtgcc gaccatgcgt tcgaccatct tgacgtggtg
      541 cgaacgccag agcgaaaaat attcgtcgtg cgcgatgaga gcttcggcca gcccgaactc
      601 ttccggtcgc gtttcgaaat gcgtgagcag ttcgaggacg gcgcgcgtgc gttggccgta
      661 gcggcgcgtg cgttcttcct tgttgagcgt cgcatcgtcg gcgggcgcgt cgaggccgcg
      721 acgacgcagg taggcgtaga aggcatcggc gagcgttggc gcgtcgaagc gcgcctgcaa
      781 gcgcgtgtgc gcgaactcat cttcgcggaa gttgtcgagc gtgccttgag ccttgaggcc
      841 ggaggcgaat tcgatctcgc gaaactgcat cgactggaag ccgctcgcgg gattgagttc
      901 gtcgcggaag ccgaggaagt tgatcggcgt catcgtctcc aacaggtgaa tttgattgac
      961 gagaaccttc tcgatctcca cgacccgcgt gagcgcgcgc gcggcgacga acgcgcgatc
     1021 ctcgttcatc aaggcgacgc tcgcatcgat ctcatggagt atctgtttga accagagttc
     1081 gtaagtttga tggatggtga taaagagcag ttcgtcgtga tgcgccggat cggagaggca
     1141 ctcctgcaac gcgatcaagt ccgccacgcg tagatatttg ttgtatgaga gcggcgcatt
     1201 ctcgccgtag ttctttgaca taatagagta atgagtaatg agtgatgagt gatgagtgat
     1261 gagtgatgag tgaagaagag tttttaactc atcactcatc actcatcact catcacttct
     1321 caataagctg cgccggtggc ttcgtgtgct tcgtaggcgc gtgtgtcgat gatgcggcgc
     1381 atttcacgta tgacgatgtc gagttcttcg tctttcgtgt agaagtgcgg cgagatgcgg
     1441 atgcccgcgc cggggcggaa gtccacgatg atctcgcggc ggatgagttc gcgcgtgacg
     1501 gcggcggcgt gttcgacgtt gacggtgacc gtgccgccgc gcctgtctgc ctcgcgcggg
     1561 ctggtgacgt tgaagcctgc ggcgtctgcg atctcgatga gcctcgttgt ctgccgcgta
     1621 ctcttggcgc gaatttgcgc gacgccgatc tcgttgatga tcgagtagcc cgcgcgtgcg
     1681 gcgtagagcg cggggatgga gggcgagccg tgcaggaagc gcgcgatgcc gggcgcgtag
     1741 gtcatccgtc cctcgaaggc gaaggggtgc tcgtgcgcca tccagccggt gactttgggt
     1801 tcgagcgtgg cgtgcaggcg cgggtgg
//
LOCUS       DASWNS010000014         4770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_78448, whole genome shotgun sequence.
ACCESSION   DASWNS010000014 DASWNS010000000
VERSION     DASWNS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78448"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VGB76_00290"
     CDS             <1..293
                     /locus_tag="VGB76_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097361.1"
                     /translation="GAMAALVYCASDYVGAWGGIELLRRRNLTVDLVAGSVTDSQMGE
                     DYIEGEFNVAAGNARRDGRRMFEIVEAKVASRKSQVASPLDENALEAVLVEA"
     gene            395..1423
                     /gene="argC"
                     /locus_tag="VGB76_00295"
     CDS             395..1423
                     /gene="argC"
                     /locus_tag="VGB76_00295"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY0097362.1"
                     /translation="MKLRVGIFGGSGYGGAELLRILLARADVEVAFVTANEHAGKAVG
                     AVHRNLYGLTDLRFVSAPEELESLPEVDCVFLALPHGQALEIAPRLHASVRVIDLSGD
                     FRLRDAREFLEHYGREHTATELQREFVYGLTETNRETIKRARLVSNPGCFATATLLGL
                     APLVAAGVLGGRVIVDAKTGSSGSGAKAATNTHHPQRMNSFYAYKPFTHQHVPEIEQE
                     LRSVGDWAGELIFMTHSLPVARGIFASIYVETRDALTTEDVRALYEDFYRESFFVRIV
                     EGSPDINPVKTTNFCDLGFAVRGRQLVVFSAIDNLVKGAAGQAVQNMNLMFGLDEKTG
                     LMMFGSNP"
     gene            1533..2717
                     /locus_tag="VGB76_00300"
     CDS             1533..2717
                     /locus_tag="VGB76_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0097363.1"
                     /translation="MMTFDEIAAHEDEFQLATYKKMKLAAARGRGAWVETSEGERFLD
                     LYGGHAVAATGHCHPHVVEAIRAQAGELLFYSNLVYSEVRARAAERLVSCAPAPLAKV
                     FFCNSGTEANENAMRMARMFTGREGIITFTGSFHGRTADAISATFLGKYREIGRPNVP
                     GHLCAEFGSIESVAALADETVAAVMLEPIQSMAGVRQAAPEFYRALRELCDARGMLLI
                     YDEVQTGIGRTGDWFFAGSAAGGEVVPDIFTLAKALGSGVPVGACLTTEAVASRIKEN
                     DLGTTFGGGMLAMAAVKATLEAIEADGMLENVRASERHLRESLAEMPRIARVRGMGLL
                     LGLEFADEIAAKVHLALLGERIIAGTSSDARVLRLLPPLCLQREEVELFVAALRRALS
                     SL"
     gene            2824..2994
                     /locus_tag="VGB76_00305"
     CDS             2824..2994
                     /locus_tag="VGB76_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097364.1"
                     /translation="MPRALSRRREREAWAAPRRAGFYVEAFARKEFYVAADDRLASGV
                     EIACVVRSKELR"
     gene            3025..4032
                     /locus_tag="VGB76_00310"
     CDS             3025..4032
                     /locus_tag="VGB76_00310"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HEY0097365.1"
                     /translation="MPRDFLTTADWTRDELERLLEAAARFKRGEESSRPLEGRSVALV
                     FFNPSLRTRASMQVGIYELGGNPVVLEPGGTSWTLEHRDGVVMDGDKTEHVAEFVRVL
                     GRYCAAIGVRTFAALKDWKEERRDPVLAAFARHSEVPIINLESAMHHPCQSLADMLTI
                     RERLGAKPQRVTLTWAWHPKPLPMAVPNSFALAAVQLGHELTIAHPPGYELDDELVGQ
                     LNEHAFDAGTTVNVTHNVEEAFDGAQVVYAKSWGSRMFYGSPEEDLEARAEYRAQWIV
                     DEAKMRRTGSGIFMHCLPVRRNVIVTDAVIDSPASVVIDEAENRLHAQKAVMKLVTGD
                     R"
     gene            4094..>4770
                     /gene="argB"
                     /locus_tag="VGB76_00315"
     CDS             4094..>4770
                     /gene="argB"
                     /locus_tag="VGB76_00315"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY0097366.1"
                     /translation="MSQPRLDLLREALPYIQRFKGKTFVVKISGKLTEDHDNLMSLCE
                     ELALLHQVGIRLCVVHGGGKQLTELAELMGVEQTIIDGRRVTDDATLDMAKMIFAGKI
                     NTDILAALRNHGVNAVGLSGIDGNIVLAERRPPKDVVNRTTGASERVDFGHVGDVVEI
                     DDRLLRVLLDHDYLPVVSPLGADSRGRVFNINADTIAAEIAVRLEAEKLILLSDVDGL
                     YLRPGESA"
BASE COUNT          918 a         1301 c         1650 g          901 t
ORIGIN      
        1 gcggggcgat ggccgcgctc gtctattgcg cttcggatta cgtgggcgcg tggggcggca
       61 tcgagttgct gcggcggcgc aatctgacgg tcgacctcgt ggccggttcg gtgaccgact
      121 cgcagatggg cgaagactac atcgaaggag agttcaatgt cgcggcgggc aacgcgcgca
      181 gggacggcag gcggatgttt gagatcgtgg aggcgaaggt cgcaagtcgt aagtcgcaag
      241 tggcaagtcc gctcgatgag aatgcgcttg aggcggtgct ggtcgaggcg tgagtccaga
      301 ggccgaagct gaaagcacaa aagcgcgaag gaagagcgga ataagttttc aaacgcactt
      361 taagcattta tctcttcaac ctgaaacttg agacatgaaa ctgagagtcg gcatctttgg
      421 cgggtcgggt tatggcgggg cggagttgct gcgcatcctg ctggcgcgcg cggacgtgga
      481 ggtcgcattc gtgacggcga acgagcacgc gggcaaagct gtcggcgcgg tgcatcgcaa
      541 tctttacggg ttgacggatt tgcgtttcgt ctctgcgccc gaagagttgg agagtttgcc
      601 ggaggtggat tgcgtctttc tcgcgctgcc gcacgggcag gctttggaga tcgcgccgcg
      661 cctgcacgcg agcgtgcgcg tgattgatct gtcgggagat tttcgcctgc gcgacgcgcg
      721 tgagtttctg gaacattacg ggcgcgagca cacggcgacg gagttgcaac gcgagtttgt
      781 ctatggcttg acggagacga accgggagac gatcaaacgt gcgcggctcg tcagtaatcc
      841 ggggtgcttt gcgacggcga cgctgctcgg ccttgcgccg ctcgtcgcag cgggagtgtt
      901 gggcgggcgc gtgatcgtgg atgccaagac gggttcttcc gggtcgggcg cgaaggccgc
      961 aaccaacacg caccacccgc agcgcatgaa ctctttctac gcctacaagc ctttcacgca
     1021 ccagcatgtg ccggagatcg agcaggaact gagaagcgtc ggcgactggg cgggcgaact
     1081 tatttttatg acgcactcgc tgcccgtcgc gcgcggcatc ttcgcctcga tctacgtgga
     1141 gacgagagac gcgttgacga ccgaagacgt gcgcgcgctg tatgaagact tttaccggga
     1201 gagtttcttc gtgcggatcg tggaaggctc gcccgacatc aacccggtga agacgaccaa
     1261 cttttgcgat ctcggtttcg ccgtgcgcgg gcgtcaactc gtcgtctttt cggcgataga
     1321 taacctcgtc aaaggcgcgg cgggtcaagc cgtgcagaac atgaacctga tgttcggtct
     1381 ggacgaaaag acagggctga tgatgttcgg cagcaatcca tgaagcagtg acaagtgacg
     1441 agtgacaggt taagaccaaa ctgcgttggc ctgtctctta catgagcttg cctgtgatga
     1501 agtgatggcg agtaaagtta aagatgaact cgatgatgac atttgatgag atcgccgcgc
     1561 acgaggatga gtttcaactg gcgacttata agaagatgaa actcgcggcg gcgcgcgggc
     1621 gcggcgcgtg ggtggagacg agcgagggcg agcgttttct cgacctctat ggcgggcatg
     1681 ccgtggcggc gacggggcat tgccacccgc acgtcgtcga agcgatcagg gcgcaggccg
     1741 gggagttgct gttctattcg aatctggttt attcggaagt gcgcgcgcgc gcggcggagc
     1801 gtctggtgag ttgcgcgccc gcgccgctgg cgaaagtctt cttttgcaat tcgggcacgg
     1861 aagcgaacga gaatgcgatg cgcatggcgc gcatgtttac cgggcgcgag ggcatcatca
     1921 cttttacggg tagctttcac gggcgcacgg cagacgcgat cagtgcgacg tttttaggga
     1981 agtatagaga gatcgggcga ccgaacgtgc ccggccacct gtgcgcggag tttggttcga
     2041 tagagtctgt ggccgcgctc gcggatgaga cggtcgccgc cgtgatgctg gaaccgattc
     2101 aatctatggc gggcgtgagg caggccgcgc cggagtttta tcgtgcgcta cgcgagttgt
     2161 gcgacgcgcg cgggatgctc ctgatttatg atgaggtgca gacgggcatc gggcgcacgg
     2221 gcgactggtt tttcgcgggc agcgcggcgg gcggcgaggt tgtgccggac atcttcacgc
     2281 tggcgaaggc tctggggagc ggcgtccccg tcggcgcgtg cctgacgacg gaggcggtcg
     2341 cctccaggat caaggagaac gacctcggca cgactttcgg cggcgggatg ctcgcgatgg
     2401 cggcggtgaa ggcgacgctg gaagcgattg aagcggatgg gatgctggag aacgtgcgcg
     2461 cgtcggagcg gcacttgcgc gagagtctgg ccgagatgcc gcgtatcgcg cgggtgcgcg
     2521 gcatggggtt gttgttaggc ttggagttcg cggatgagat cgccgcgaaa gtacatctgg
     2581 ccttgctcgg cgagcgcatc atcgcgggca cttccagcga tgcgcgcgtg ctccgcctgt
     2641 tgccgccgct ctgtttgcag cgagaagagg ttgaactctt tgtcgctgcg ctcagacgcg
     2701 cgttgtcgtc tctctgagca gttttgatcc cgcgcaaact attatgagct tccgtgtgcg
     2761 cagggcgaga cgggaggacg acgaagcgga cgggcggctg cacgtcgcat ccaacagaga
     2821 gttgtgccgc gcgcattatc gcgccggaga gagagagagg cgtgggctgc gccgcgcagg
     2881 gctggtttct acgtcgaagc gtttgcgcgg aaggagttct atgtcgcggc ggacgacaga
     2941 ttggctagcg gggttgagat cgcgtgcgtc gtgaggtcga aggagttgcg ttaaagagag
     3001 tgccgggtga ggctgcgaaa aactatgccg agagattttc tgacgactgc cgactggacg
     3061 cgcgacgaac tcgaacgcct gctcgaagcg gcggcgcgat tcaaacgcgg agaagagagt
     3121 tcgaggccgc tcgaaggcag gtccgtcgcg ctcgtcttct tcaatcccag cttgcgcacg
     3181 cgcgcctcga tgcaggtcgg catttacgaa ctcggcggca acccggtcgt gctcgaaccc
     3241 ggcggcacga gttggacgct cgaacacagg gacggcgtgg tgatggacgg cgacaagacc
     3301 gagcacgtcg cggagtttgt gcgcgtgctc ggacgttatt gcgcggccat cggcgtgcgc
     3361 acgttcgccg cgctcaaaga ttggaaagag gagcggcgcg atccggtact cgcggccttt
     3421 gcgcgccaca gcgaagtgcc catcatcaat ctcgaatcgg cgatgcacca cccgtgccag
     3481 tcgctcgcgg acatgttgac gatcagggag aggctcggcg cgaaaccgca gcgcgtaacg
     3541 ctcacttggg catggcatcc gaagcccctg ccgatggccg tgccgaacag cttcgcgctc
     3601 gcggcggtgc agttggggca tgaactgacc atcgcgcacc cgcccggcta cgaactcgac
     3661 gacgaactcg tcgggcaact caacgaacac gcgttcgacg cgggcacgac ggtcaacgtc
     3721 acgcacaatg tagaagaagc cttcgacggc gcacaggtcg tctacgcgaa aagctggggc
     3781 agcaggatgt tctacggctc gccggaagaa gacctcgaag cgcgcgccga atatcgcgca
     3841 caatggatcg ttgacgaagc gaagatgcga cgcaccggga gcggcatctt catgcactgc
     3901 ctccctgtgc gccgcaacgt gatcgtcacg gacgccgtca tcgattcccc cgcctccgtc
     3961 gtcatagacg aagccgaaaa ccggctacac gcgcagaaag cagttatgaa attagtgaca
     4021 ggtgacaggt gacaggttaa agcagaaact ttgtcttcat cctgtcacct gtcacctgtc
     4081 acacggagga tttatgagcc agccgcgact cgatctgttg cgcgaagcgt tgccttatat
     4141 tcagcgtttc aagggcaaaa ctttcgtcgt caaaatctcc ggcaaactga cggaggatca
     4201 cgacaacctc atgtctttgt gcgaggagtt ggccttgctg catcaggtgg gcatacggct
     4261 ctgcgtggtg catggcggcg ggaagcagtt gacggagctg gcggagttga tgggcgtcga
     4321 gcagacgatc atcgacgggc gacgtgtgac cgacgatgcc acgctcgaca tggcgaagat
     4381 gatcttcgcc ggaaagatca acacggacat tctcgccgcg ctgcgtaatc atggcgtgaa
     4441 cgccgtcggc ctgtcgggca ttgacggcaa catcgtgctg gccgaacgtc gcccgccgaa
     4501 ggatgtggtc aatcgcacga cgggcgcgag cgagcgcgtg gacttcgggc acgtcgggga
     4561 tgtcgtggag attgacgacc gcttgctgcg cgtgctgctc gaccacgatt acctgcccgt
     4621 cgtctcgccg ctcggcgcgg actcgcgcgg gcgcgtcttc aacatcaacg ccgacaccat
     4681 cgccgcagag atcgccgtgc gcctcgaagc cgaaaagttg atcctcttga gcgacgtgga
     4741 cggcctctac ctgcgccccg gtgaatccgc
//
LOCUS       DASWNS010000015         6210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_311884, whole genome shotgun sequence.
ACCESSION   DASWNS010000015 DASWNS010000000
VERSION     DASWNS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311884"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            540..1037
                     /locus_tag="VGB76_00320"
     CDS             540..1037
                     /locus_tag="VGB76_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEY0097367.1"
                     /translation="MSEPFLSEIRIMSFNFAPKGWAMCNGQLLPINQNQALFSLLGTT
                     YGGDGRVNFALPDLRSRVPIHTGSGHTLGEKGGEQAHTLTMSEMPAHNHIVQGSSANG
                     ASVLPTDGVLAAAAGLYGDPVQLTSLLPASVTNVGGSQAHLNMQPFLTLTFCIALQGI
                     FPSQN"
     gene            1055..1558
                     /locus_tag="VGB76_00325"
     CDS             1055..1558
                     /locus_tag="VGB76_00325"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEY0097368.1"
                     /translation="MAQPYVGEIRMFAGNFAPAGWMFCEGQLLPISENETLFQLIGTT
                     YGGDGQSTFALPDLRGRLPIHQGNGFILAETGGAEEVTLTIQQIPVHNHAFLGSKGVG
                     NQASPVGFIPAASNTVSLYKAAAPVNNMAAQSVLPTGGSQPHTNFQPYLCIDFIISLF
                     GIFPSQT"
     gene            1573..2106
                     /locus_tag="VGB76_00330"
     CDS             1573..2106
                     /locus_tag="VGB76_00330"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEY0097369.1"
                     /translation="MADPFVAEIRIFPFTFAPKGWAFCNGQLLPLSQNTALFSLLGTT
                     YGGDGKSNFALPDLQGSAAMHPGQGPGLSLHDLGETGGSETVTLLQSEMPAHSHFIVA
                     AGNPPPANAGTPDPSVALARSSGTTAYQASPFSNLGNMNPQLLSISGASLPHNNMMPY
                     LTINFCIALQGVFPPRG"
     gene            2625..>6210
                     /locus_tag="VGB76_00335"
     CDS             2625..>6210
                     /locus_tag="VGB76_00335"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0097370.1"
                     /translation="MPVGFAATTTQTIQSKSRNAGKRQPERKETAPATQTARAQKAPP
                     VVPSALTTSNITATKMDTIVGDSLGDGKAQAGDTIKYDITITNNSATDNALNVALNDT
                     IDSNTTLVGDSIHAQPQARNDSYTTVGNTLLEVGVPAGAAPAVRVAGSAFDNDGNATD
                     PSTFVSATQPANGTVTFNADGTFTYLPNAGFTGPTDSFTYTIRNSADSTLTDMATVTI
                     TITSRVWYVDNAAAAGDGRSTSPFNSLSSVNNAASDADAASDYIYLFTGNAAYTTGLV
                     LENNQQLIGNGVALVVPINATPTTLRAAGTRPTLSNGTGNALTFASNNTVQGLNLTAA
                     ANAAIFGSGISGTNNLSDANITTTGSGTAVSLTNQAGTFNYSGGGGSSISGNSSGTAV
                     LISGGNGNITFFGVPISQNSGRAIDIQTRTGGTVSFENGSTVTETAGSTDAVVLRNNT
                     GGSVINFSNNVNLTTNAGRGLYTDNSTGSFTLNMNAAGNNINATGGAAIDVEDIIADL
                     DFQTLTSTNSVVAGGSAGVRIVNLSNAPVGRSVTVTGATTVTNSAATGVIVQGNAAAI
                     TFATLNSAPAAGQIGILADTNSGTTTATAGTITATDAIAVSVLNSPLIMTLTSVSANN
                     TGDSDPGVSLNGMTGTLVMSGGSLVGGNAQAFFANAQNGSITYNGTISQANAFRLIEV
                     TNKTGGTVNFGGALTATGANSTGIRLNANGNTTFNFTGGMNLSTGVNDAFTATGGGTV
                     NITQNNGAIVNTLTTTTGTALNVTSTNIGASGLTFRSINSTGASAKNGVILNTTGTTA
                     GTHGGLTITGNGGTCSSAATCTGGNIANKNSGADASTTEGTGIYLKDARDVNLTNVFL
                     NDFANYAIYGSNVTNFTFTTSRITGSNGTSSNPINEGCIRFDGLLGNALITGSNIGNT
                     NTAGGATNALHINNASGTLTLLDIQNSTIFNQNASNSPANSGQDAVFFGSPSGATGTM
                     NLRMNNTLLTHARQFLMQVNIQGTKTANLEITNNTLHNQHPSVVNAGGGSNITGGGTD
                     VYVKYLISGNSFRHTPGVTNGGRSLTIGHASGSAAFDGRFINNTIGQTGVVSSGAGNA
                     ADGLGIFASGNNSPSMGGSKALIQGNTIQRYGEAGILVNARQGSATLDVTILGNIIRE
                     PGTAALGAFAAIWVNSGALAADTNIVNLVVGSATVAANKNTMTNSDPSN"
BASE COUNT         1399 a         2164 c         1641 g         1006 t
ORIGIN      
        1 gagtccggcg gcaacagcgg agacacacgc ggactgcaaa aagccacggc gggagatgga
       61 catataagtt ggctcctaac gaattgtaga cgactcgata gacggtgctt acaggatgtt
      121 tactgatggt gcttgctgaa gatggtgctt gcgaggggat ggaacgtagc ggaaaagcct
      181 acattgccct tcgcgcagaa gtgtaatcct tctgctaaaa aatgacaacg ttcatttgga
      241 tcatttaaca ctgaaacata tctttatcct tgaaattgct acgatcaagc cgtacaatgc
      301 ttgcctcgcg gggatcaagc ctttcaggaa tcctgtaaga acccaagttc tatcaacctc
      361 gactgacaac ccgggatacg cgtcagcgaa tttcgaaccc agggtcaagt agtaccaacc
      421 agcaacgaac ccttcagtat tgtagcttcg cttttctttg tcgaagtgtg ctctttaaaa
      481 ctcaattcca actactcaga ggactgctct ctgaagtccc tttaagtaag gagaaatgca
      541 tgtccgaacc gtttctcagt gagatcagaa tcatgtcctt caacttcgcg ccgaaaggtt
      601 gggcgatgtg caacgggcaa ttgctgccca tcaaccagaa ccaggcgctc ttctcgctgc
      661 tcggcacgac ctacggcggc gacgggcgcg tcaacttcgc gctgcccgat ctccgctcgc
      721 gcgtgccgat tcacaccggc agcggacaca cactcggcga aaaaggcggc gagcaggcgc
      781 acacgctcac gatgagcgaa atgccggcgc acaaccacat cgttcagggg tcgtcggcca
      841 acggcgcgtc ggtcttgccg acggacggcg tgctggccgc cgccgcaggt ctttacggcg
      901 atcccgtcca actgacctcg ctcctccccg ccagtgtaac gaacgtcggc ggcagccagg
      961 cgcacctgaa tatgcagccg ttcctaaccc tcacgttctg catcgccctc cagggcattt
     1021 tcccatccca gaactaagga gaagatagac caccatggca caaccctatg tcggtgaaat
     1081 tagaatgttc gcgggcaatt tcgcccccgc gggatggatg ttttgcgaag ggcagttact
     1141 ccccatctcc gagaacgaga cgttattcca attaatcggg acaacctacg gcggcgacgg
     1201 ccaatcaacg ttcgcacttc cagacctgcg cggacgcctt ccaatccatc agggcaacgg
     1261 cttcatcctg gcggaaacgg gcggcgcgga agaggtgacg ctcaccatcc agcagattcc
     1321 ggttcacaac cacgccttcc tcggctcgaa gggcgtcggc aatcaggcca gccccgtcgg
     1381 ctttataccc gccgcgtcaa acacggtgtc gctctacaag gcggcggctc cggtcaacaa
     1441 catggccgct cagtccgtgc ttcccaccgg aggcagccag ccgcacacca actttcagcc
     1501 gtacctgtgc attgatttca tcatctctct cttcgggata ttcccgtcgc aaacttaagg
     1561 aggccgtcca ttatggcaga cccatttgtc gctgaaatcc gtatctttcc gttcaccttc
     1621 gcgcctaagg gatgggcatt ttgtaacggg caattattgc cgctctcgca aaacaccgcg
     1681 ctcttctcgc tcctcggcac gacctacggc ggcgacggca agtcgaattt cgccctcccc
     1741 gacctacagg gaagcgcggc gatgcatccc ggtcagggac cgggactctc cctccacgac
     1801 ctcggcgaaa ccggcgggtc ggaaaccgtc acgctcctcc aatcggagat gcccgcgcac
     1861 agccacttca tcgtggcggc tggcaatccg ccgcccgcca atgccggtac gcccgatccg
     1921 agcgtcgcgc tggcgcgttc gagcggaacg accgcctatc aggcttcgcc cttctcgaac
     1981 ttgggcaata tgaacccgca actgctcagc atctcgggcg ccagcctgcc gcacaacaat
     2041 atgatgccct acctgacgat taacttctgc atcgcgctgc aaggcgtctt cccgccccgc
     2101 ggttaaacgg acggagagtt tcacccgaaa gcaacgacaa accggaggcg gaacgcgcac
     2161 tcgtcaatcc cctcaacgcg aagcggcggg agccagaccg ttcggcaata aaggaacggt
     2221 gctcccgtcg ctttcaaaaa tttcgctgcg gggtggtgaa ttccgggcag agcatgttat
     2281 aaagtttatg gtggcgcacc cggccaccgc tacctgtcag acgtttcacc agagacgcct
     2341 ctataaattg accgtttctc tacccagacc tctgcgcata cagttctgta taaacgtcac
     2401 gactacttcg aaaatcaaag tgcaaaaact ggctcgtacg tcgtcgttta aatatcaagc
     2461 acaaccctaa acctgagaca tttctacagg ccgccctcgc acaatgtgga aatgcagtcc
     2521 ccttaaggag agttatgaag agcttcaccc ccatatccag ctttagaaat tccagagcct
     2581 ttctcgcaag cctcctcact tgcatcatgt tagcgactcc ccttatgccc gtcgggtttg
     2641 cggcgacgac aactcaaacg attcaatcaa aatcgaggaa cgccgggaag cggcaaccgg
     2701 agcgcaagga gacggctccg gcgacgcaaa ctgcgcgtgc gcagaaagcc ccgccggtcg
     2761 tccccagtgc gctaacgacc tccaacatca cggccaccaa aatggacacc atcgtgggcg
     2821 actcgctcgg cgacggcaaa gcgcaggccg gcgacacgat caaatacgac atcacgatca
     2881 cgaacaacag cgcgaccgac aacgccctca acgtcgcgct caacgacacc atcgactcga
     2941 acaccacgct cgtcggcgac tcgattcacg cgcagccgca ggcgcgcaac gactcgtaca
     3001 ccacggtcgg caacacgctg ctcgaagtgg gcgtgcccgc aggcgcagcc ccggcggtga
     3061 gagtcgcggg cagcgcgttc gacaacgacg gcaacgcgac cgacccgagc acgttcgtct
     3121 ccgccaccca gcccgccaac gggactgtga ccttcaacgc cgacggcact ttcacctatc
     3181 tgccgaatgc cgggttcacc ggcccgaccg actccttcac ctacacgatc aggaacagcg
     3241 ccgacagcac gctgaccgac atggcgaccg tcacgatcac gatcaccagt cgcgtctggt
     3301 atgtggacaa cgcagcggcg gctggcgacg ggcgttcgac ttcccctttc aactcgctct
     3361 cgtcggttaa caacgcggca tcggacgctg acgcagccag cgactacatc tacctcttca
     3421 ccggcaacgc cgcctacacg accggcctcg tccttgagaa caatcagcaa ctcatcggca
     3481 acggcgtcgc actcgtcgtc cccatcaacg ccacgccgac cacgctccgc gccgcgggca
     3541 cgcgcccgac gctcagcaac ggtacgggca acgccctgac cttcgccagc aacaacaccg
     3601 tgcaggggtt gaacctgacg gcggcggcca acgcggccat cttcggttcg ggcatcagcg
     3661 gcacgaacaa cctctcggac gcgaacatca ccaccaccgg ctcaggcacc gccgtgagcc
     3721 tgacgaatca ggcgggcacc ttcaactact cgggcggcgg cggcagttcc atctcgggca
     3781 acagttccgg cacagccgtc cttatcagcg gcggcaacgg aaacatcacg ttcttcggcg
     3841 tccccatcag ccagaacagc gggcgcgcga ttgacatcca gacgcgcacg ggcggcaccg
     3901 tcagtttcga gaacggttcg accgtcaccg agacggctgg ctcgaccgac gctgtcgtcc
     3961 tgcgcaacaa cacgggcggc tccgtcatca acttttcgaa caacgtcaac ctcacgacga
     4021 acgctggacg cggcctctat acagacaaca gcaccggcag tttcacactc aacatgaacg
     4081 ccgccggtaa caacatcaac gcgacgggcg gcgcggccat cgacgtggaa gacatcatcg
     4141 ccgacctcga cttccagacg ctcacctcga ccaacagcgt cgtggcgggc ggctcggctg
     4201 gcgtcagaat cgtcaatctc tccaacgccc ccgtgggtcg cagcgtgacg gtcacgggcg
     4261 caacgaccgt gacgaactcg gcggccaccg gcgtcatcgt gcagggcaac gcggcggcga
     4321 tcacattcgc cacgctcaac agcgcacctg cggcaggaca gatcggcatc ctcgccgaca
     4381 ccaactccgg cacgaccacc gcgacggctg gcacgatcac ggctaccgac gccatcgccg
     4441 tcagcgtctt gaattcgccg ctcattatga cgctcaccag cgtctcggcg aacaacacgg
     4501 gcgacagtga tccgggcgtc tcgctgaacg gcatgaccgg cacgctcgtg atgagcggcg
     4561 gctcgctcgt cggcggcaac gcgcaagcct tcttcgccaa cgcgcagaac ggcagcatca
     4621 cctacaacgg caccatctcg caggcgaacg ccttccgcct tatcgaagtg acgaacaaga
     4681 ccggcggcac ggtcaacttc ggcggcgcgc tcacggcgac cggcgcgaat agcacgggca
     4741 tccgcctgaa cgccaacggc aacacgacat tcaacttcac cggcggcatg aacctctcga
     4801 ccggcgtgaa cgacgccttc accgccaccg gcggcggcac cgtcaacatc acgcagaaca
     4861 acggcgcaat cgtcaacacg ctgaccacga cgacgggcac cgcgctcaac gtcacgagca
     4921 cgaacatcgg cgcgagcggc ctcaccttcc gcagcatcaa ctcaaccggc gccagtgcga
     4981 agaacggcgt catcctcaac acgacgggca cgacggcggg cacgcacggc ggcctgacca
     5041 tcaccggcaa cggcggcacc tgctcctcgg cggccacctg caccggcggc aacatcgcca
     5101 acaagaacag cggcgcggac gccagcacca cggaggggac gggcatctac ctcaaagacg
     5161 cgcgcgacgt gaatttgacg aacgtgttcc tcaacgactt cgccaactac gccatctacg
     5221 gcagcaacgt caccaacttc accttcacca cgagccgcat cacgggttcg aacggcacga
     5281 gcagcaaccc catcaatgag ggctgtatcc gcttcgacgg cctgctcggc aacgcgctca
     5341 tcacgggcag caacatcggc aacaccaaca cggcgggcgg cgcgaccaac gccctgcaca
     5401 tcaacaacgc gtcgggcacg ctcaccttgc tcgacatcca gaacagcacc atcttcaacc
     5461 agaacgcgag caattcacct gccaacagcg gtcaggacgc cgtcttcttc ggctcgccct
     5521 ccggcgcgac cggcacgatg aacctgcgca tgaacaacac gctgttgacg cacgcgcgcc
     5581 agttcctcat gcaagtcaac attcagggca cgaagaccgc caacctcgaa atcaccaaca
     5641 acacgctcca caaccagcat ccgtccgtcg tcaacgcggg cggcggctcg aacatcacgg
     5701 gcggcggcac ggacgtatac gtcaaatatc tcatcagtgg caacagcttc cgccacaccc
     5761 ccggcgtcac aaacggcggt cgctccctga ccatcggcca cgcttccggc agtgccgcct
     5821 tcgacggtcg cttcatcaac aacaccatcg gccagaccgg cgttgtctca tcgggcgcgg
     5881 gcaatgcggc agacggcctc ggcatcttcg ccagcggcaa caacagcccc tcgatgggcg
     5941 gctcgaaagc cctgattcag ggcaacacca tccaacgtta cggcgaggcg ggcatcctcg
     6001 ttaacgcccg tcagggcagc gccacgctcg acgtgaccat cctcggcaac atcatccgcg
     6061 agccgggcac agccgccctc ggcgcgttcg ccgccatctg ggtcaactcc ggcgcgctcg
     6121 ccgccgacac caacatcgtc aacctcgtcg tcggctcggc caccgtcgcc gccaacaaga
     6181 acacgatgac gaactccgac ccgagcaacg
//
LOCUS       DASWNS010000016         6136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_260029, whole genome shotgun sequence.
ACCESSION   DASWNS010000016 DASWNS010000000
VERSION     DASWNS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260029"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VGB76_00340"
     CDS             complement(<1..921)
                     /locus_tag="VGB76_00340"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEY0097371.1"
                     /translation="MKEKIRIASGQGFWGDMLDAPVRQVKGGQIDYLMLDYLAEVTMS
                     IMQKQRTRDPRAGYARDFVPLMRDILPDCVQKNIRVTANAGGVNPVGCAEAVRDVARE
                     LGLAGRLRIGTVTGDDIMSRLDDFLARGIELRNMDTGEPLSKVRDAIQSANVYLGAWP
                     MVEALNRGAQVVITGRATDTGLTLAPIIHEFGWLADDWDRLSAGTIAGHIIECGAQCS
                     GGNCQYDWQSIPDLADVGFPIVEAAPDGTFVVTKHEGTGGLVSIPTIKEQLVYEMGDP
                     HEYITPDCVADFTSIRLEEAGRNRVRVHGIK"
     gene            complement(1156..1845)
                     /locus_tag="VGB76_00345"
     CDS             complement(1156..1845)
                     /locus_tag="VGB76_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097372.1"
                     /translation="MKRSSNISSKMIGLLILFVAVIFVCADLPTALAQNTNSGTTGGD
                     TTMQESNTNTGSVRRGRRRARRRARRRARRQANANATDANANASTDAGNANMAGDTTT
                     TPDANTNTGTAGGTQGGGGTTGMGTFGDVADLSGTYTGTINYPEGGMTGEATLTITTN
                     TFTITAGSATQSGTLSARSWPGYTALSMRFGETLPATIVSTRAWHRGRSLRIQSVRGE
                     SRAFTFNGRSQ"
     gene            complement(2258..3910)
                     /locus_tag="VGB76_00350"
     CDS             complement(2258..3910)
                     /locus_tag="VGB76_00350"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY0097373.1"
                     /translation="MMKSETPSSSGAAEKRTRMRALVEDYERLAAKLRRGGGADKIER
                     QHTQGKLTARERLALLLDPNTYAQEIGLLVAYDEYKGGAPSAGVVTATGRVGGREVVV
                     VANDATVKAGSWWPETIKKILRAQEIAMRSRVPIIYLVDSAGVNLPYQGGVFPGQYGA
                     ARIFYYNSIMRRYLHVPQISAVMGPCVAGGAYLPALSDIIVMVEGTSFMGLGGANLVK
                     GATGQTIDNETLGGARTHNEISGVAHYRVANDEECITKIREFVSELPLTQSRALSVSE
                     SAEASRSVEELYSLIPEDHRQPYDARAVLQCIIDGGHWDEFQADYAREMITGHARIRG
                     IQVGVIANSRGMFRAAGGGQPKFGGIIYTESAEKVAYFIDVCNRHRTPLVFIQDVSGF
                     MVGAEAEHSGIIRAGARFVEAMATASVPKIVLTINHASGAGYYAMAGQGFDPDFIFSW
                     PTGRMGVMEGDSAVQALFGTQLDKLKREGKEPDEELKREMARVREDYERQLDAKYAAA
                     RGFVDAVIAPDETRDALELALRVSANYDGAHLGHFVLPPTLV"
     gene            complement(4141..5511)
                     /locus_tag="VGB76_00355"
     CDS             complement(4141..5511)
                     /locus_tag="VGB76_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097374.1"
                     /translation="MSKRNMRRGWRRPYAAAFVAVALAGAVWTVGAPQVAAQVAAPQA
                     PTAAVNPRSASVVAATSEVLKETSEIRQLEVLRAVKSGAQSRAEIERMLVRNLDEQTT
                     PADLHASELALKKLGLVPADFQFRSFLIALLTEQVAGYYEPRTQEFYLADWIDLDGQQ
                     PVMAHELTHALQDQHFNLRRFEKWPKGDSDAELAAHALVEGDATLVMTFYVMRDLKRV
                     AAMMKSMSAEGSSSEKIERAPRVIRESILFPYKQGMDFASQLYRRGGWPLISEAYKAL
                     PQSSEQILHPEKYFAREAPVKVNLPELVELLGRNWTRLDYDVNGEWSYFQILDEFLRA
                     EKVSQKAVAGWGGDRFALYENRATREVLLAQLTAWDTEQDAVEFYDAYLNRTERRYKD
                     AVADTRASIPTGTRRVWRTPKEGLVVLERQGARVAILEGVPEKVDVNALLKKVLAGTQ
                     TVQASQ"
     gene            5606..6115
                     /locus_tag="VGB76_00360"
     CDS             5606..6115
                     /locus_tag="VGB76_00360"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0097375.1"
                     /translation="MMAETAMDDPLFDVWHETPVRVRYAETDRMGVVYHANYLVWFEI
                     GRTEFCRARGFAYRDMEENSDAFLVVAESYCRYKAPAFYDDELIVRTHITELRRRSVR
                     FGYEILRATDGLIIAEGETGHVVTDGHGRVKSMPEQYRDLLSAVPEEDIHTSQLPQRI
                     EPVPEGNAP"
BASE COUNT         1086 a         1972 c         1832 g         1246 t
ORIGIN      
        1 cttgatgccg tggacgcgca cgcggtttcg tcccgcctct tccagccgga tcgaagtgaa
       61 atcggcgacg caatcgggcg tgatgtattc gtgcgggtcg cccatctcat agacgagttg
      121 ttccttgatc gtaggaatgg agacgaggcc gcccgtgccc tcgtgcttgg taacgacgaa
      181 cgtgccgtcg ggcgcggctt cgacgatggg aaagccgaca tccgcgagat cgggaattga
      241 ttgccaatcg tactggcagt tgccgcccga acattgcgcg ccgcattcga tgatatgccc
      301 cgcgatagtt ccggcggaga gtctgtccca atcgtctgcc agccagccga actcgtgaat
      361 gatcggcgcg agcgtcagtc cggtatcggt cgcgcgcccg gtgatcacta cctgcgcgcc
      421 gcgattcaga gcctcgacca tcggccacgc gccgagatag acgttcgcgc tctggatcgc
      481 atcgcgcact ttggagagcg gctcgcccgt gtccatgttg cgcagttcga ttccgcgtgc
      541 gagaaagtcg tccaggcgac tcatgatgtc gtcgcccgtc acagtaccga tgcgcagacg
      601 cccggcgagt ccgagttcgc gcgccacgtc gcggacggct tcggcgcaac ccacaggatt
      661 gacgccccct gcgttcgccg tcacgcgaat atttttctgc acgcagtcgg gcagaatatc
      721 gcgcatgagc ggaacgaagt cgcgcgcgta tccggcgcgc gggtcgcgtg tgcgctgctt
      781 ctgcataatg gacatcgtga cttccgccag gtagtcgagc atcaggtaat cgatctgccc
      841 gcctttcacc tgacgcacgg gcgcatccag catgtcgccc caaaaaccct gcccgctcgc
      901 aatgcgaatc ttctctttca tcttgtgcac aaaactctga atccgtcgcg tctaaaagta
      961 aaaaggtaaa agttaaagca agaagcagtt cttacctttt gccttttacc tttttggaag
     1021 tgcttacaaa ctcgcgctca ctgcctcaat ccttcgcgcc tttcgctttg actttgcgcc
     1081 tccggcgcga actgtttttc acgccggagg cgcaaagcga agagaacccg aaggcgcaaa
     1141 gagaacgccg ctcaactact gggaacgccc gttgaaagtg aacgcgcgcg actcgccgcg
     1201 cacgctctgg attctcaaac tgcgtcctct gtgccaggcg cgcgtcgaga cgatggtggc
     1261 gggcagcgtt tcaccgaagc gcatcgagag cgcggtgtag cccggccaac tgcgcgcgct
     1321 gagcgtgccg ctctgcgtgg ccgaaccggc ggtgatcgtg aaagtgttgg tcgtgatggt
     1381 cagcgtcgct tcgcccgtca tgccgccctc cgggtaatta atcgttccgg tgtaggtgcc
     1441 agacaggtcg gccacgtcgc cgaaggtgcc catgcccgtt gtgcccccgc cgccctgcgt
     1501 gccgcccgcc gtgccggtgt tggtgttcgc gtccggcgtg gtggtcgtgt cgcccgccat
     1561 gttcgcgttg cccgcgtcgg tggacgcgtt ggcgttggcg tcggtcgcgt tggcgttcgc
     1621 ttggcgacgt gcgcggcgac gtgcgcggcg acgtgcacgg cggcgtccac ggcgcacgct
     1681 gccggtgttc gtgttcgact cctgcatcgt ggtgtcgccg ccggtcgtgc cgctgttggt
     1741 attttgcgcc agcgcggtgg gcaggtctgc gcagacgaag atcaccgcga cgaaaaggat
     1801 caagagcccg atcattttgc ttgagatgtt gctgcttctc ttcatggtaa gtgtcatcct
     1861 ccggtggtat atcgcgtcgc acctcgctat aggccgacgg ctaagcgttt tcgtgagacg
     1921 cctcttgcga ccagtctccc cgtgctcgtc ccgatctctg ccgacttcac ccggcgcttg
     1981 cggaagccgc cggtgaagaa cttgctgact gccgcccggc acgcttcgca cgtcggtcgc
     2041 tggcgcactc cgcgctggct acagcgtcaa acaagctgcc gttaaacttc gtctcgcact
     2101 gaagccgcac tacgggggag agaaaaacag ttccgattta gctgcttaaa ctttgcgcgt
     2161 agtttctcac aagatggata cggttgcacg aaaaatcgtg cggcgacgcg cgaacccggc
     2221 ggattttccc ggcctccggc gagcggaatt ttcatcctca cacgagcgtc ggcggcaaga
     2281 cgaaatggcc gagatgcgcc ccgtcgtagt tcgcgctcac gcgcaaggcc agttccagcg
     2341 cgtcgcgcgt ctcgtccggc gcgatcacgg cgtccacgaa cccgcgcgcc gcagcgtatt
     2401 tcgcatccaa ctgccgctcg taatcctcgc gcacacgcgc catctcgcgc ttcaactctt
     2461 catccggctc cttgccctcg cgcttcaact tgtcgagttg cgtgccgaag agagcctgca
     2521 ctgccgagtc gccctccatc acgcccatgc ggcctgtcgg ccaactgaaa atgaaatccg
     2581 ggtcgaagcc ctgccccgcc atcgcgtagt aacccgcgcc cgacgcgtgg ttgatggtga
     2641 gtacaatctt cggcacgctg gccgtcgcca tcgcttcgac gaagcgcgcg cccgcgcgga
     2701 tgatccccga gtgttccgct tccgcgccga ccatgaagcc cgacacgtcc tgtatgaaga
     2761 cgagcggcgt gcgatggcga ttgcacacgt ctatgaagta agcgaccttc tcagccgact
     2821 ccgtgtagat gatgccaccg aacttcggct ggccgccgcc cgccgcgcga aacatgccgc
     2881 gactgttggc gatgacgccc acctgtatgc cgcgtatgcg cgcgtgtccg gtgatcatct
     2941 cgcgcgcgta gtcggcctga aactcgtccc agtggccgcc gtctatgatg cactggagca
     3001 ctgcgcgcgc atcgtaaggc tgccggtgat cttccgggat gaggctgtag agttcttcca
     3061 ccgaacgcga cgcctccgcg ctctccgaga ctgagagcgc acgcgattga gtcaagggaa
     3121 gttcggacac gaactctctg atcttcgtga tgcactcttc gtcgttcgcc acgcggtaat
     3181 gagcgacgcc ggaaatttcg ttatgcgtgc gtgcgccgcc tagagtctcg ttgtcaatcg
     3241 tctgcccggt cgcgcctttg acgaggtttg cgccgccgag tcccatgaag gaagtgccct
     3301 cgaccatcac gatgatgtcg gagagcgcgg gcaggtacgc gccgcccgcc acgcatggac
     3361 ccatcaccgc ggagatttgc gggacgtgca ggtaacgccg catgatcgag ttgtaataga
     3421 agatgcgcgc cgcgccgtac tggccgggaa agacgccgcc ctgatacggc agattcacgc
     3481 ccgccgaatc caccagatag atgatcggca cgcgcgagcg catggcgatc tcctgcgcgc
     3541 gcagaatctt cttgatcgtc tccggccacc acgaaccggc cttcaccgtc gcatcgttgg
     3601 cgacgaccac gacttcgcgc ccgccgacgc gtcccgtcgc cgtcaccacg cccgccgacg
     3661 gcgcgccgcc cttgtactcg tcgtaagcga cgagcaggcc gatctcctgc gcgtaggtgt
     3721 tcgggtctaa gaggagcgcg aggcgctcgc gcgcggtgag tttgccttgc gtgtgctggc
     3781 gttcgatctt atctgcgccg ccgccgcgac ggagtttcgc agcgagccgc tcgtagtctt
     3841 cgacgagtgc gcgcatgcgc gtgcgcttct cagccgcccc ggaagaagat ggtgtttctg
     3901 acttcatcat caagcgtcgc cgtccgggcg tcgtgcccgg ccagcaggca caactcggcg
     3961 gcgttcatcg ctgcttaaat atttatgacc aaagatgctt caacataaca tacgagcgcg
     4021 acgcgagaca acgcacctta accataagaa agcgtcgcat agttaaagct gccaacgcgg
     4081 ctttaactat gcgacgcttc gtgatctctt acctgtcgcc gctcgcgcgg cttggacaaa
     4141 ttattgactc gcttgaactg tttgggttcc cgccagcacc tttttcaaca acgcgttcac
     4201 gtccaccttc tccggcacgc cttcgaggat ggcgacgcgt gcgccctgtc tctccaaaac
     4261 caccagccct tctttcggcg tgcgccacac gcggcgcgtc cctgtgggga tgctggcgcg
     4321 cgtgtcggcg acggcatctt tgtaacgccg ctcggtgcga ttgaggtagg cgtcgtagaa
     4381 ctccacggcg tcctgttcgg tatcccacgc cgtgagttgg gcgagcaaga cctcgcgcgt
     4441 cgcacggttt tcgtagagcg cgaagcggtc gccgccccat cccgccacgg ctttctgcga
     4501 cactttctcc gcgcgcaaaa attcgtcgag gatctggaaa taactccact cgccgttcac
     4561 gtcatagtcg agtcgcgtcc aattcctgcc gagcagttcg acgagttccg gcaagttaac
     4621 tttgacgggc gcttcgcgtg cgaaatattt ttccgggtgc aggatctgtt cggacgactg
     4681 cggcagagct ttgtaggctt cggagatgag cggccaaccg ccgcgtctat agagttgtga
     4741 agcgaaatcc attccctgct tgtagggaaa gaggatcgat tcgcgtatga cgcgcggcgc
     4801 acgctcaatc ttttcagacg aactaccctc cgcgctcatt gatttcatca tcgcggcgac
     4861 gcgcttcaaa tcacgcatca cgtagaaggt catcacgagc gtcgcgtcgc cttcaacgag
     4921 ggcgtgcgcg gcgagttcgg cgtcggagtc gcccttcggc catttctcga agcggcgcag
     4981 gttgaagtgc tggtcttgca gggcgtgcgt gagttcgtgc gccatgacgg gttgctggcc
     5041 gtcgaggtcg atccagtctg cgaggtaaaa ctcctgcgtc ctcggttcgt agtagcccgc
     5101 cacctgttcg gtcagcagcg cgatgagaaa ggaacgaaac tggaaatcgg cgggcacaag
     5161 tccgagcttc ttcaacgcga gttcgctcgc gtggaggtct gcgggcgtcg tctgctcgtc
     5221 gagattgcgc acgagcatcc gctcgatctc ggcgcgcgat tgcgccccgc tcttcaccgc
     5281 gcgcaacact tccagttgcc tgatctcgct cgtctctttc aagacttcgg aggtggccgc
     5341 caccacgctt gcgctgcgcg gattgaccgc tgcggtcggt gcctgcggcg cggcgacctg
     5401 cgcggcgacc tgcggcgcgc ccaccgtcca aaccgctccc gcgagcgcga cggcgacgaa
     5461 tgctgcggca taaggtctgc gccagccgcg cctcatgttt ctctttgaca tctcgaacct
     5521 ttcgctttta tagtccgcag acacgcggct catttcaggt agtgtagatt gctccccgcc
     5581 ggagagcaat cagcgggagg cttttatgat ggcagaaacg gctatggacg atcctctgtt
     5641 cgacgtgtgg cacgaaaccc ccgtgcgcgt gcgctacgcc gagacggaca gaatgggcgt
     5701 cgtctatcac gccaactacc tcgtctggtt tgagatcggt cgcacggagt tttgccgtgc
     5761 gcgcggcttc gcctaccgcg acatggaaga gaactccgac gcctttctcg tcgtcgccga
     5821 aagctattgc cgctacaaag ctcccgcctt ttacgatgac gaactgatcg tgcgcacgca
     5881 catcacggaa ctgcgccgtc gctccgtccg cttcggttac gaaatcctgc gcgcgacgga
     5941 cggcttgatc atcgcagaag gcgaaaccgg ccacgtcgtc acggacgggc acgggcgcgt
     6001 caaatcaatg cccgaacaat accgcgacct gctctccgcc gtccccgaag aagacataca
     6061 cacttcgcaa ctcccgcaac gaatcgaacc cgtgcccgaa ggcaacgcgc cgtgagaaga
     6121 agggatgaag gatgag
//
LOCUS       DASWNS010000017        17972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_467576, whole genome shotgun sequence.
ACCESSION   DASWNS010000017 DASWNS010000000
VERSION     DASWNS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17972
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467576"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VGB76_00365"
     CDS             <1..1117
                     /locus_tag="VGB76_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0097376.1"
                     /translation="PLTSDLLRIARSRTATQNRGLLEAALARYTTPLLLVLIGMSKHG
                     KFQTTCEYAVTRALLGGLGLLPRRAAVATGRAAGRLAHKFAGGLRRTGEINLRLAFPE
                     MEARERERVLRGSFDNLGRLLGEFSQFPSATPESLRALVEYDEVGLKHLREAEAQGRG
                     VIFLTAHLGAWEFLSFAWSALEYPISFLVRPIDNARVEELIEGIRTRYGNKAINKKMA
                     ARQALRVLREGGTLGILADLNTHPNEGVFVPFFGHLACTTSGVATLALRTDAVVIPTC
                     AVWDEQRGRFFFHGDPPVELVRTGDDRRDVETNTANFAAAIERMIRAYPEQWLWIHKR
                     WRTRPDGEPDFYNHGKLFDTKESHLTAPTGHNSLSKS"
     gene            complement(1635..2405)
                     /gene="fabG"
                     /locus_tag="VGB76_00370"
     CDS             complement(1635..2405)
                     /gene="fabG"
                     /locus_tag="VGB76_00370"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY0097377.1"
                     /translation="MSDDVLQRKPFDGRAAIVTGATRGIGRAIALELARRGAHVSFNY
                     AKSAEAAEELRVEIESLGVRVLATQCDVAQTEAVASMVKATKEAFGSIDFLVNNAGIT
                     RDNLILRMKEEDWDTVVDTNLKGPWNFARAALRTMLRQEEGGSVLNISSISGLVGMAG
                     QSNYSASKAGLVGLTKALAKEVASRKVTVNALALGLVSTDMSGALDEGYQTKLLEQIP
                     LGRFAEPQEVAEIACFLVSPAARYITGQVIQVDGGLAM"
     gene            complement(2476..3351)
                     /gene="galU"
                     /locus_tag="VGB76_00375"
     CDS             complement(2476..3351)
                     /gene="galU"
                     /locus_tag="VGB76_00375"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY0097378.1"
                     /translation="MPQKIRKAVFPAAGLGTRFLPATKASPKEMLALVDKPLIQYGVE
                     EAVASGVESILIITGRGKGVIEDHFDISFELEHTLRQRGKDQLLALARSVSEIARISY
                     VRQSEALGLGHAILQARDFVGDEPFAVLLPDDIMDCETPALKQMLRVYEEYDAPVLGT
                     MQVAGEAISRFGAIDAEEVAEGVYKIRDMVEKPPFAEAPSDLAIIGRYILTPDIFDEI
                     ERTERGTGGEIQITDAMRSLLKRRPFYAVRFEGVRHDAGDKLGFLIATVEFALKRPDL
                     APEFREYLRSLDLNK"
     gene            3546..4841
                     /gene="serS"
                     /locus_tag="VGB76_00380"
     CDS             3546..4841
                     /gene="serS"
                     /locus_tag="VGB76_00380"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY0097379.1"
                     /translation="MLDLNFVRDNLERVRAALEARRAPVAALEDFARMDAERRRVIAE
                     SDELNAARNAASRAIGALMKEGRREEAEAERRAVGELKDRIAALDRLREEAETNMRQL
                     LSTLPNLPHESVPVGADEAANVEVRRWGAPPAFDFAPKDHVDLGASLGILDLERATKI
                     AGARFAILYGAGARLERALINFMLDVHTGEAHNYRETLPPFIVNQKALFGTGQLPKFE
                     QDLFKLTDERELYLIPTAEVPVTNIRGDEVLDEQELPLKFAAYTPCFRSEAGSYGRDT
                     RGLIRQHQFDKVELVKLALPENSYDELEGLTRDAETILQKLGLHYRVVALSTGDLGFS
                     SAKTYDIEVWLPSQNAFREISSCSNFEAFQARRAQIRFKRASGGKPEYVHTLNGSGLA
                     VGRTWLAVLENYQQADGSILIPEALRPYMNGMEKIEAAQ"
     gene            complement(4924..6726)
                     /gene="sppA"
                     /locus_tag="VGB76_00385"
     CDS             complement(4924..6726)
                     /gene="sppA"
                     /locus_tag="VGB76_00385"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY0097380.1"
                     /translation="MSRSGKIFLWVAGLLVALALVVVIGAVMLLALFADDSPDVRDNS
                     VLVLKVEGALPDYAPEDPFASRFLGRDDRSLTRLLSNIKKAKADKRIGAILLELDMTM
                     IGWGKADEIRDTIADFRSSGKPVYAYMEYGTNKEYYLATACDRIYVAPIGDLFIVGLA
                     ADVMSLGGSLDKLGVKADFYQIGKYKTAPEQYTRKEMSEASREVTNAILDEFFGRYVS
                     TIAATRKKSEADVRTLIDNAPLGAWEAKAAGLIDDAKYRDEVEEELKQRLGYKASDKL
                     RKVRESDYRRVTPESVGLAQGERIAVIFASGPIGGGESDDGSAFSSQSTGSDTVVKAL
                     NDARDDKTIKAIILRVDSPGGTSYASDVIWHAVEKAKAQKPVVVSMSDVAASGGYYIS
                     ATASKIIAQPSTITGSIGVYAGKPVIKGFYDWIGVNNEYVLRGKQAGMFRETEPFNAD
                     ERAKFEGMLKSFYYDDFLPKVAKGRGRDVEYVDSIAQGRVWTGTQAKERGLVDEFGGM
                     DKAVEVAKGLANIPADKNVRRIVFPAPRSFLQQFLGGGGAEGSANAKAREQRAAIESA
                     LPEDMRRALRYMSILENMKRGETMAMLPFDLQIK"
     gene            7194..9482
                     /locus_tag="VGB76_00390"
     CDS             7194..9482
                     /locus_tag="VGB76_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEY0097381.1"
                     /translation="MHQLRFAVRRQRTCLYLLVAAALFGALALTGTPPRVASATGAHL
                     LKDDQDASAAAIPVIAPGSAYRQTNFVSDVPGIAFIEDPLLVNPWGISLTASSPFWVA
                     NTGTSTSTLYRGDVGGVVFFKNAGLAGITIPGGLPTGTVANPTAADFVVPGACAAAPC
                     GANFIFASITGNIVGWDPNAPAAGSITGVIAASHPGHVYTGLAIGNNGAGNFLYAPDF
                     ANGRIDVFNNAYALQPAASFPFADPTIPTTPGNRYHPYNVQAVGSSLYVTYAKVGAGG
                     LDEEGIGNGFVRRFNLNGVRDLTFGINNGPLNSPWGIVVAPASFGIFGGALLVGNFGA
                     GNPSIHAFNPTTGAFLGTIQDEGGHGIVIDELWAIVFGNGGNGGDPNTLYFAAGIAKE
                     EHGLFGSLKPTTASATSLVEFSSSDYAIGEGNSSIQITVTRSGNVSGSATVNYATFDE
                     SRPGHANQKTDYEIAVGAVSFAPGETSKTFKVILVDDKFDENDETIDLALSNPTGTGV
                     GLGSVNRSKLLITDNDTGAAATNPIDETAFFVRQHYLDFLGREPDAGGFNAWVNVLNG
                     CAVGNTACDRVTVSRSFFESPEFQSRGYFIIRAYLAAYGRNPLYGEFMGDLSRLSGAT
                     AEETNALRAGFTATFVTRNEFVATLGALTNAQYVDRLIANSGVANANRNALVASLDAL
                     QKTRAQVLQEIVDSFQFVNDTPTFSRAYILAQYFGYLRRNPDQAGYNMWLNYLMANPR
                     DFRTMVHGFVNSNEYRSRFGAS"
     gene            complement(9633..10742)
                     /gene="mnmA"
                     /locus_tag="VGB76_00395"
     CDS             complement(9633..10742)
                     /gene="mnmA"
                     /locus_tag="VGB76_00395"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY0097382.1"
                     /translation="MKIAVAMSGGVDSSAAAAILQAAGHELVGFTMQLWNQNRNINVD
                     ENGDPLPSRCCSLDDVYDARRVAGELGFPFYVLNLEQDFERDVVQPFVTSYLEGETPI
                     PCVACNSRLKFASLDRLAQSLGCEKVATGHYARVSEDAATNRYRLFRGEDPNKDQSYF
                     LWELTQAQLAHAMFPLGEMSKAEAREAARRFNLAVAEKAESQEICFVPDGDYAGFIDR
                     YLAAEGASERLPGAGEIVDTNGRVIGEHQGVHRYTVGQRRGLGISRPLPLYVVKVDAE
                     RNRVVVGESDELLAREFTAAGVTWIAFDEPDAPVRAEVRVRYRHTPAHATIEPLADRR
                     VRVTFDEPQRAISPGQATVFYRGDEVLGGGWIVKR"
     gene            11021..11827
                     /locus_tag="VGB76_00400"
     CDS             11021..11827
                     /locus_tag="VGB76_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEY0097383.1"
                     /translation="MPERAIEEYTVTDEPYYLPVASEVEMFEAANAARLPVMLKGPTG
                     CGKTRFVEHMAWRLGRPLITVACHEDLSATDLVGRFLLEGEETVWHDGPLTSAVKSGA
                     ICYLDEVVEARKDTVVIIHPLTDHRRRLPIEKRGTILDAPEDFMLVVSYNPGYQSVLK
                     DLKQSTRQRFVSLEFDYPPAELETEIVAREGGIDLQAARDLVQIGHKIRNLRGHGLEE
                     GVSTRLLIYAAQLIARGIAPVAAAEVALASPITDDRELQRSIREIITTVI"
     gene            11839..12402
                     /locus_tag="VGB76_00405"
     CDS             11839..12402
                     /locus_tag="VGB76_00405"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0097384.1"
                     /translation="MNEALAAHFLEDALAIFRKYKKLAEDAIAQIDDEEFFARIDAEA
                     NSVAVIVKHLAGNMRSRWTDFLTTDGEKATRARDAEFEIEPETTRAEVMRWWEEGWSC
                     VFTAVEPLEPADLEHTVTIRGEPHTVVKAINRQLTHYTYHTGQIVLLAKHFKAESWSS
                     LSIARGQTEAFNRSLREGESTERLSEK"
     gene            12449..15715
                     /locus_tag="VGB76_00410"
     CDS             12449..15715
                     /locus_tag="VGB76_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0097385.1"
                     /translation="MSKPDDKDAPNASKANEETAQSQPLADVLPEQFAEVRSASERRN
                     VAGLVREIGQLPADRARAALEVSAGLASLSLRVGLEFLRAAPGAAQVLEAAELREWGE
                     MGRRLAMADVETGANFFRAGVAQLLAVPQATRPLLFQVCARQLPLSTSIATETFRLAP
                     ELARAAPDEALLATTFEVALEIARRSARHSADFLAASFDAFAHLKNYERAGAGGRENE
                     VARAALHLAGVFAQRAGGVAADAWTNIPAATSGLEAAHALALLRHTETFLERGGAAAS
                     HVLVAGGEVLRLAPQVFADWTALLHTVAAQGNAPLVSLARHSPLFFRALPARDARGAA
                     LTQGTSDLARRVIAATRAVAEVDAEAAISCFRSAPTALRATSLEHFEQWARDGLALKR
                     NDARTRRSYYALETRQSNAALNEGGAGLALETVAPTLRLYVEGLTGRAVEVASLAAVP
                     DEARIGDGRTIHLPAAVSEFADDERNFRLYKVLAAHAAGQIEFDTHARAGNDTAASAS
                     LRAAFASIAELYAEENRDAQDAFALDGYINDPAQSEPALAPAEEARRARNSRRRMPKE
                     SDYRAVLALFPLPTLAARIFGTLENGRIDRRLRHAYRGLARDLDLVRTHLRASRPRIA
                     TIPLTLVPFELLFQITLCGGALDDARSFYGQIVSELESIVAGYLNGAEASVADTLMAT
                     NRVYTLFQSVTPQATEQQEQQSETQDGGASGAESLLEDASARKPREPEGEPRNDPPPN
                     DARELFNAWAKSNSSEADAAEQLFGAERWTQSQSPEQDLEPGEVAYSYDEWDRELADT
                     RVNWCRVVEKRVRRGDRSFVELTRTRYRGVISSIKHQFQMMRPESLLRIKNELDGDDY
                     DLDAVINYVADRRADGRQSERLYTKRLRRERDVAVSFLLDQSSSTARTIGRHPLQPYT
                     HPGRRIIEIEKEGLVLMSEALEAVGDAYAIYGFTSEGRRNVKFYVVKDFHERHSEEIE
                     RRIGGINYQHNTRLGAAVRHAAARLRVQEARTRLLIVLSDGRPYDHDYGDARYAREDT
                     REALRQARVAGVTPFCITIDRDSEAELRDLYGEVGYTIIDDVLSLPERMPAIYRRLTT
                     "
     gene            16038..16460
                     /locus_tag="VGB76_00415"
     CDS             16038..16460
                     /locus_tag="VGB76_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097386.1"
                     /translation="MRRTLALLSLLALATFGAACAASDNANTGANANNANNMNATTGT
                     TGTGTTGTGATTGTGTTGTTGTADNMNTTGGTTGGTTGGTTGTGGNTNTGGNTNMGGN
                     ANRGNTNAGNGNTGGGNGNMRTGNTNTGTGTGANRNTP"
     gene            complement(16644..>17972)
                     /locus_tag="VGB76_00420"
     CDS             complement(16644..>17972)
                     /locus_tag="VGB76_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0097387.1"
                     /translation="DEVFAGRLDRFGLIDPDNGGRVHAGMVGLYFRNESASGATFKAD
                     AFVSRSLFDLYSNFTFFLTDEVNGDEIQQHDSRLQEGANLQYLRPYKLFKHLALITVG
                     ANYHDNQINVGLYPTVARSPIAVGEAVSPGVTTRPLTDARARVTNASGYVQHGIDLLH
                     GHLHIEAGVRYDYFRFKVDDRAAPALSGAQSAARFQPKFSAAYNPADNFPATFYFNYG
                     RGISSQDARGVVGNPSAPKVSTTDFYQLGTSHKFKRFSLVTDLFLIDRSNEQVYIPDD
                     GSFEFRGASRSYGYEFKTSAQLTKHLAFNGGLTQVSNAFFRATRPRVYVDSAPHTVAN
                     AALTLSGYRGFDAALNYRHTGSYRLDGEDATIRASGLDVLDFSLRKRIRRFVDFNLSI
                     DNLTNKRYFETQNYFESRLRPGDPVVARIHGTPGYPFGVTAGFTFRFAGK"
BASE COUNT         3462 a         5800 c         5350 g         3360 t
ORIGIN      
        1 acctctcacc tctgacctct tacgtatagc acgcagccgc acggcgacgc aaaatcgcgg
       61 tttgctagag gccgcgctgg cgcgctacac tacgcctctc ctcttagtgc taatcgggat
      121 gagtaaacac ggaaaatttc agacaacctg cgaatacgcc gtcacgcgcg ctctgctcgg
      181 cgggctgggt ctgctgccgc gccgtgcggc ggtggcgacg ggtcgtgcgg cgggcaggct
      241 cgcacacaaa ttcgcgggcg gcctgcgtcg cacgggcgag atcaatctgc ggctcgcctt
      301 ccccgaaatg gaggcgcgcg aacgcgagcg tgtgttgcgc gggtcgttcg acaacctcgg
      361 acgcttgctc ggcgagttca gccagtttcc aagtgcgacg ccggagagtt tgcgcgcgct
      421 cgtggagtac gatgaagtcg ggcttaaaca tctgcgtgag gcggaagcac aggggcgcgg
      481 cgtcattttc ctgacggcgc atctgggcgc gtgggaattt ttgagtttcg cctggtcggc
      541 gctcgaatac ccgatcagtt ttctcgtgcg cccgattgac aacgcgcgcg tcgaggagtt
      601 gatcgagggc atccgcacgc gctacggcaa caaggccatc aataagaaga tggccgcgcg
      661 tcaggcgctc cgcgtcctgc gcgaaggcgg cacgctcggt atcctggccg acctcaacac
      721 gcacccgaac gaaggcgtct tcgtcccctt cttcggccac ctcgcctgca ccacttcggg
      781 cgtcgctacg ctcgccctgc gcaccgacgc ggtcgtcatc ccgacctgcg ccgtgtggga
      841 cgaacaacgc gggcgtttct tctttcacgg cgacccgccc gtcgaactcg tccgcaccgg
      901 agacgaccgc cgcgatgtcg aaaccaacac ggcaaacttt gccgccgcca tcgaacgcat
      961 gatccgcgct tatcccgaac agtggctctg gattcacaaa cgctggcgca cgcgtcccga
     1021 cggagagcct gatttctaca atcacggaaa actattcgac acaaaagagt cacatttaac
     1081 cgctcccacc ggacataaca gcctctccaa gtcgtaagcg caagattttt atcgtttttt
     1141 cgctttcccg cgccccttct ttgtcctttt ctgacaacat ttagcctcta accgggagtt
     1201 gtattgcaaa tttgtcactt ttttttgtat tttatgcttg acacgtaaca atcgtttagt
     1261 gtaatttcac acacgtcgga gggcagtgag ttttataaca aagtttttca gcagattgcc
     1321 cttcgagatt tttgaaatca gtgaaattcg gccaagtgcc acggtaacgc aaaggctgct
     1381 ttacgaagca ttatattttt cgagccttgc aattataact cgcccgtacc aacgcaccct
     1441 gaccaaaatg ccggatcgcc cgtaagtgac gatctccagc gccaagtcaa cgccaccctg
     1501 aatctccgag cctgcccaaa aaattcagcc tcgttagtcc cccaagctaa cgaggctgct
     1561 tctctttgta gccgaccaat ttccgccttc agcagccagc ctccagcggc gagacgcttc
     1621 acagactaaa gttttcacat cgcgagtccg ccgtccacct gtatcacctg cccggtgatg
     1681 taacgcgcgg cgggtgaaac tagaaagcac gcgatctccg cgacttcctg cggttccgcg
     1741 aaacgaccga gcggtatttg ttcgagcaac tttgtctggt agccttcgtc gagcgcgccc
     1801 gacatgtctg tggagacgag gccgagcgcg agcgcgttga ccgtcacctt gcggctcgcc
     1861 acctctttcg ccagcgcctt cgtcaaaccg acgagtcccg ctttcgacgc ggagtagttc
     1921 gactgtccgg ccattcccac cagcccgctg atggaggaga tgttcaagac cgacccaccc
     1981 tcttcctgcc tgagcatcgt gcgcagagcc gcccgcgcga agttccaggg gcctttcaaa
     2041 ttcgtgtcca cgacggtgtc ccaatcctct tccttcatgc gcaggatcaa attgtcgcgc
     2101 gtgatgcccg cgttgttgac gaggaaatct atgcttccga acgcctcttt ggtcgccttg
     2161 accatcgagg cgacggcttc cgtctgcgcc acgtcgcact gcgtcgcgag aacgcgcacg
     2221 ccgagcgact cgatctccac gcgcaactct tcagccgcct ccgcgctctt cgcgtagttg
     2281 aaggaaacgt gcgcgccgcg ccgcgccaac tccaacgcga tggcgcgacc gatgccgcgc
     2341 gtcgctcccg tcacaatggc cgcccgcccg tcgaaaggtt ttcgctgtaa aacgtcgtcg
     2401 ctcaaatttt catcctccgt ttaagaccgg aaatcttctc atctccaaag ttcacgcgcc
     2461 tcgtgaattt cacgtctact tgttcaagtc caacgaacgc agatattccc tgaactccgg
     2521 cgcgaggtct ggacgcttca aggcgaattc gacggtggcg atgaggaagc cgagtttgtc
     2581 gcccgcgtcg tggcgcacgc cttcgaagcg cacggcgtag aagggtcgcc gtttgagcag
     2641 cgaacgcatc gcgtccgtga tctgtatctc gccgcccgtg ccgcgctctg tgcgttcgat
     2701 ctcgtcgaag atgtccggcg tcaggatgta acgcccgatg atcgccagat ccgaaggggc
     2761 ttcggcgaag ggtggtttct ccaccatgtc cctgatcttg tagacgcctt cggcgacttc
     2821 ctccgcgtca atcgcaccga agcgcgaaat ggcctcgccc gccacctgca tcgtgccgag
     2881 gacgggtgcg tcgtattctt cataaacgcg gagcatctgt ttgagcgcgg gagtttcgca
     2941 atccatgatg tcgtcgggca gcaggacggc gaagggttcg tcgccgacga aatcgcgcgc
     3001 ctgtaagatg gcgtgcccga gtcctaatgc ttcactctgg cgcacgtagc tgatgcgcgc
     3061 gatctcggag acggagcgtg cgagcgcgag cagttggtct ttgccgcgct ggcgcagggt
     3121 gtgttcgagt tcgaaggaga tgtcgaagtg atcttcgatg acgcccttgc cgcgcccggt
     3181 gatgatgagg atcgactcga cgccggaagc gaccgcctcc tccaccccgt actggatgag
     3241 cggcttatcg acgagcgcga gcatctcttt gggcgacgct ttcgtcgcag gcagaaagcg
     3301 cgttcccaga cccgcggcgg gaaagacggc tttacgaatc ttctgtggca tgttcttctg
     3361 actccccttg tcgaagctgg aatactatca atcgccgccg acaaaaagag tcgcggcgca
     3421 accggaagcc gacgcttgac acgcgcgcgc acagtcggct agcgtgacga acttcgttga
     3481 ctaatactgc tggtactgct ggcgaaaaga tagaacttca taccacacgt taaaggacag
     3541 tacaaatgct cgatctcaac ttcgtccgcg acaatctgga acgcgtccgc gccgcactcg
     3601 aagcacgccg cgcgcccgtc gccgcgctcg aagactttgc acgcatggat gccgagcgtc
     3661 gccgcgtgat cgcagagtcc gacgaactca acgccgcgcg caatgctgcg agccgcgcta
     3721 tcggcgcgtt gatgaaagaa gggcggcgcg aagaggccga ggcagagcgg cgagcggtcg
     3781 gagagttgaa ggatcgcatc gcggcactgg accgcctgcg cgaagaggcc gagacaaaca
     3841 tgcgtcagct tctctcgacg cttcccaatc tcccgcacga gtctgtccct gtcggcgcgg
     3901 acgaagcggc gaacgtggaa gtgcgtcgct ggggcgcgcc gcccgcgttc gatttcgctc
     3961 cgaaggatca cgtagacctc ggcgcgtcgc tcgggatact cgacctcgaa cgcgcgacga
     4021 agatcgcagg cgcacgcttc gccattctct acggcgcggg cgcgcgtctc gaacgcgctc
     4081 tcatcaactt catgctcgac gtgcacacgg gcgaggcgca caactacagg gagacgctgc
     4141 cgcctttcat cgtcaaccag aaggcactgt tcggcacggg tcaactgccg aagttcgagc
     4201 aggacttgtt caagctcacg gacgagcgcg aactctacct gatcccgacg gcggaagtgc
     4261 ccgtcaccaa tattcgcggc gatgaggtgc tcgacgaaca ggaactgccg ttgaagtttg
     4321 cggcctacac gccctgcttc cgttccgagg cgggcagcta cgggcgcgac acgcgcgggc
     4381 tgattcgcca gcaccagttc gacaaggtgg aactggtgaa actcgcgctc cccgaaaatt
     4441 cctacgacga actcgaagga ctcacgcgcg atgccgagac gatcctgcaa aagttagggc
     4501 tgcactatcg cgtcgtcgcg ctctcgacgg gcgacctcgg attcagttcg gccaagactt
     4561 acgacatcga agtctggttg ccctcccaga atgccttccg cgaaatctct tcgtgctcca
     4621 acttcgaggc gtttcaggcg cggcgcgcgc agatacgctt caaacgggcg tcgggcggca
     4681 agcccgaata cgtccacacc ttaaacggct ccggcctcgc cgtcgggcgc acatggctcg
     4741 ccgttctgga aaactatcaa caagcggacg gctcgatcct gatccccgaa gcgttacgcc
     4801 cgtacatgaa cgggatggag aagatcgagg ccgcgcagta gtcgaaagca gtagatgtca
     4861 aaagcgtaga ggccgtgtga acgtcacacg gcctctacgc ttctgacatc tactgtctat
     4921 tgattacttg atctgtaggt cgaacggcag catcgccatc gtctcgccgc gtttcatgtt
     4981 ttcgaggatc gacatgtagc gcagggcgcg gcgcatgtct tcgggcaagg ccgattcaat
     5041 cgcggcgcgc tgctctctgg ctttcgcgtt cgcggagcct tctgcgccgc cgccgcccaa
     5101 gaactgttgc aggaacgaac gcggcgcggg gaagactatg cggcgcacgt tcttgtcggc
     5161 gggaatattc gccagcccct tggcgacttc gacggccttg tccattccgc cgaactcgtc
     5221 aaccagaccg cgctcctttg cctgcgtgcc cgtccagacg cgcccctgcg cgatggaatc
     5281 cacgtactcg acatcacgcc cgcgcccctt cgccaccttc ggcaaaaagt cgtcgtaata
     5341 gaaactcttg agcatccctt cgaacttggc gcgctcgtcg gcgttgaagg gttcggtttc
     5401 gcgaaacatt ccggcctgtt tgccgcgcag cacatactcg ttgttgacgc cgatccaatc
     5461 gtagaaacct ttgatgacgg gtttgcccgc atagacgccg atggagccgg tgatggtcga
     5521 aggttgtgcg atgatcttcg acgcggtggc ggagatgtag taaccgccgg aggccgccac
     5581 gtcgctcatc gagacgacga cgggcttctg cgcttttgct ttctccaccg catgccagat
     5641 cacatccgac gcgtaactcg tcccgcccgg actatccacg cgcaggatga tcgccttgat
     5701 cgtcttgtcg tcgcgcgcgt cgttcaacgc tttgacgacc gtgtccgagc cggttgactg
     5761 gctgctgaac gcgctgccgt cgtcggactc gccgcccccg atgggaccgg aagcgaagat
     5821 gacggcgata cgctcgccct gcgcgagtcc cacggattcg ggagtcacgc ggcgataatc
     5881 ggattcacgc accttgcgca gtttgtccga agctttgtag ccgagccgct gcttgagttc
     5941 ttcttcaact tcatctcgat acttcgcgtc gtcgatgagt cccgccgcct tcgcttccca
     6001 cgcgccgagc ggggcgttgt cgatgagcgt gcgcacgtct gcttccgact tcttgcgcgt
     6061 cgcggcaatc gtcgagacgt agcgcccgaa gaactcgtcg aggatcgcgt tcgtcacttc
     6121 cctgctcgcc tcggacatct ccttgcgcgt gtactgttcg ggcgcggtct tatattttcc
     6181 gatctggtaa aagtccgcct tgacgccgag tttgtcgagc gagccgccga gcgacatcac
     6241 atccgcggcg aggccgacga tgaagaggtc gccgatgggc gcgacgtaga tgcggtcgca
     6301 ggcggtggca aggtaatact ccttgttcgt gccgtactcc atgtaggcat agacgggttt
     6361 gcccgacgag cgaaagtccg cgatggtgtc gcgtatctcg tcggccttgc cccagccgat
     6421 catcgtcatg tcgagttcga gcaggatcgc gccgatgcgt ttgtccgcct tcgccttttt
     6481 gatgttcgac agcaggcgcg tcagcgaccg gtcatcgcgc cccagaaagc gactcgcgaa
     6541 agggtcttcg ggcgcgtaat cgggaagcgc gccttccacc ttcaacacca gcacgctgtt
     6601 atcgcgtacg tccgggctat cgtcggcgaa caaggccaac aacatcactg cgccgatcac
     6661 gacgacgagc gcgagcgcga cgagcaagcc cgccacccat aaaaagattt tcccactgcg
     6721 agacatagcc ataagattgc ttcaactgct cctgaaatca gccctcccct tgctgcatcc
     6781 gggcaccccg ggaggtctat gtgaatacgg gaaaatggcg gaaaagttta gctacgcggc
     6841 gcgtggcgga gagcctgcga gatcgttatc cgccatccct gcccctgacg cctccattct
     6901 tcgcacacca tatcgcataa cgcaaatgtc ttgacaggcg tgagcgtcgg ctatagagtc
     6961 gtcgccgctt cccccaaagc cacttgaaca catttatcgc ccagacagaa actgccgacc
     7021 ttccgggcac gtggcttcaa ttgcatttct caaaccgcga tcacgggctg cgcaccttat
     7081 atccagcact ttcgccattg tctcttattg ataccgccga acttctctgt ccagcggcat
     7141 ccctgcgttt agtgtaatta attaaccacc tcgtccgatc acggagaatc tacatgcatc
     7201 aactgcgctt cgccgttcgc cgccagcgaa cctgtctcta tctgttagtg gccgctgcgc
     7261 tcttcggcgc actcgctctc accggcacgc ctccgagagt tgccagcgcg acgggcgcgc
     7321 atctgctcaa ggacgatcag gatgcgtccg ccgcagccat ccctgtcatc gctcccggct
     7381 cggcctaccg tcaaaccaac ttcgtctccg acgtgccggg catcgccttc atcgaagacc
     7441 cgctgctcgt caacccgtgg ggcatctcgc tcaccgcgtc cagccctttc tgggtggcta
     7501 acacaggcac gagcacgtcc acgctctatc gcggcgatgt cggcggcgtc gtatttttca
     7561 agaacgcagg acttgcgggc atcacgattc ccggcggcct cccgaccggc acggtcgcca
     7621 acccgacggc ggctgacttc gtcgtccccg gcgcgtgtgc cgccgcaccg tgcggcgcaa
     7681 acttcatctt cgcctcgatc acgggcaaca tcgtaggttg ggacccgaac gctcctgccg
     7741 ccggttcgat caccggcgtg atcgccgcga gccaccccgg ccacgtctac acgggtctag
     7801 ccatcggcaa caacggcgcg gggaattttc tctacgcccc cgatttcgcc aatggcagga
     7861 ttgatgtttt caacaacgct tatgcgctcc aaccggcggc cagtttcccc ttcgccgacc
     7921 cgactatccc gacgactccc ggaaacaggt atcatcctta caacgttcag gccgtgggca
     7981 gttcgctcta cgtcacctac gccaaggtcg gcgcgggcgg gctggacgaa gaaggcatcg
     8041 gcaacggctt cgtccgcagg ttcaacttga acggcgtccg cgatctcacg ttcggcatca
     8101 ataacgggcc gctcaattcg ccctggggga tcgtcgtcgc gcccgcgagt ttcggcatct
     8161 ttggcggtgc gctgctcgtc ggcaacttcg gtgcgggcaa tccgagcatc cacgctttca
     8221 acccgacgac cggcgcgttc ctcggcacga ttcaggacga gggcgggcac ggcatcgtga
     8281 tcgacgaact gtgggctatc gtcttcggca acggcggcaa cggcggcgac ccgaacacgc
     8341 tctacttcgc cgcaggcatc gccaaagagg agcacggact cttcggctcg ctcaaaccga
     8401 cgacggcttc cgccacgtcg ctcgtcgagt tcagttcgtc tgactatgcc atcggcgagg
     8461 gcaacagcag tattcagatc accgtcacgc gttccggcaa cgtctccggc tcggccaccg
     8521 tcaactatgc caccttcgac gagagccgcc ccggccatgc aaatcaaaag accgactatg
     8581 agatcgccgt cggcgcggtc agcttcgccc ccggcgaaac gagcaagacc ttcaaggtga
     8641 tcctcgttga cgacaagttc gacgaaaacg acgaaacaat cgatctcgct ttgagcaatc
     8701 cgaccggcac aggcgtcggc ctcggcagtg tgaataggtc gaagctgctc atcacggaca
     8761 acgacacagg agcggctgcg accaacccga ttgacgagac ggccttcttc gtccgccagc
     8821 actatctcga cttcctcggg cgcgagcctg atgcgggcgg cttcaatgcg tgggtcaacg
     8881 tgctcaacgg ctgcgctgtg ggcaacaccg cctgcgaccg cgtcacggtt tcgcgttcgt
     8941 tcttcgagtc gccggagttt cagagtcggg gctacttcat catccgcgct tatctggccg
     9001 cctacgggcg caacccgctc tacggcgaat tcatgggcga cctcagccgc ctgagcggcg
     9061 cgaccgcgga agagacgaac gctttgcgcg cgggcttcac cgccaccttc gtcacgcgta
     9121 acgaattcgt cgccacgctc ggcgcgctca ccaacgcgca atatgtagac cggctgatcg
     9181 ccaatagcgg cgtggcaaac gccaaccgca acgcgctcgt cgcctcgctc gacgcgcttc
     9241 aaaagacgcg cgcacaggtc ttgcaggaga tcgtggacag cttccagttc gtcaatgaca
     9301 cgcccacgtt cagccgcgcc tacatcctcg cgcaatactt cggctacctg cgccgcaatc
     9361 ccgatcaggc tggctacaac atgtggctca actacctgat ggcgaatccg cgcgacttcc
     9421 gcacgatggt tcacgggttc gtcaactcga acgagtatcg cagccgcttc ggtgcgtctt
     9481 agcagcaacc ccctctgctg aggcaaaaag gaacgaggac agtttggtta aactgtcctc
     9541 gttccttttt tgctgcgccg acacaaagca tgtccggctc gcccgcaact aatgattaaa
     9601 acaacggcgg ctcgcctgca ccacctcgac gcttatcgtt tcacgatcca gccgccgccg
     9661 agcacctcgt cgccgcgata aaagacggtc gcctgtccgg gcgagatggc gcgctgcggt
     9721 tcgtcgaagg tgacgcgcac gcgcctgtcc gcgagcggtt cgatggtcgc gtgcgcgggc
     9781 gtgtggcgat agcgcacgcg cacctccgcg cgcacgggcg cgtccggttc atcgaacgcg
     9841 atccaggtga cgcccgccgc cgtgaactcg cgcgccagca actcgtcgct ctcgcccacc
     9901 acgaccctgt ttcgctccgc gtcaactttg acgacgtaga gcggaagcgg gcgcgagatg
     9961 cctaacccgc gacgctgccc gaccgtgtag cgatgcacgc cctgatgctc gcctatgacg
    10021 cgcccgttcg tgtccacgat ctcgcccgcg cccggcagac gctcgctcgc gccctccgct
    10081 gcgagataac gatctatgaa gcccgcgtaa tcgccgtccg gcacgaagca gatctcctgc
    10141 gactccgcct tttccgccac cgcgagattg aaacgccgcg ccgcctcgcg cgcctccgct
    10201 ttcgacatct cacccagcgg aaacatcgcg tgcgcgagtt gcgcttgcgt caactcccag
    10261 aggaaataag attgatcctt gttcgggtct tcgccgcgaa agagcctgta acggttcgtc
    10321 gccgcgtcct ccgagacgcg cgcgtaatgt cccgtcgcca ccttctcgca gccgaggctt
    10381 tgcgccagcc ggtcgagcga agcgaacttc aggcggctgt tgcaggccac gcacggaatc
    10441 ggcgtctcgc cctccagata actcgtgacg aacggctgca ccacgtcgcg ctcgaaatcc
    10501 tgttcgagat tcagcacgta gaagggaaag cccaactctc cggccacgcg gcgcgcatcg
    10561 tacacgtcgt cgagcgagca gcagcgcgag ggcaacgggt cgccgttttc gtccacgttg
    10621 atgttgcggt tctggttcca caactgcatc gtgaagccga cgagttcgtg ccccgccgcc
    10681 tgcaagatcg ccgccgccgc cgaagaatcc acgccgccgc tcatcgccac cgcaatcttc
    10741 ataacttaaa aacggtgacg agtgacgggt gacgagtgac aagtaaaaac ctgttctttc
    10801 ttgtcacttg tcacccgtca ctcgtcactt cctcatgttt gccgcgcttt cacggcgaat
    10861 gttaagatgc tcggacagac aacaaatgtg tccaacaaca aacagttaag gatacccgaa
    10921 acagagcgaa acgtcagcct ctcaactgtc gaagccgcgt gccggagttt gcctggaaac
    10981 agagactttt agttttacgt tcagttgaga tcattgcctt atgcccgaaa gagccatcga
    11041 agaatatacc gttacggacg agccttatta cctgcccgtg gcgagcgaag tggagatgtt
    11101 tgaagcggcg aacgcggcgc gtctgcccgt gatgttgaag gggccgacgg gctgcggcaa
    11161 gacgcgcttc gtcgagcaca tggcgtggcg tctcggtcgt cccttgatca cagtggcctg
    11221 ccacgaagac ttgtccgcca ccgatctcgt cggtcgtttc ctgctcgaag gcgaagagac
    11281 ggtctggcac gatggaccgc tcacctccgc cgtcaagagc ggcgcgatct gttatctcga
    11341 tgaagtggtc gaagcgcgca aggatacggt cgtcatcatt caccctctga cggatcatcg
    11401 ccgccgcctg cccatcgaga agcgcggcac gatcctcgat gcgccggagg acttcatgct
    11461 cgtcgtctcc tacaatcccg gctaccagtc ggtcttgaaa gacctcaaac agtctacgcg
    11521 ccaacgcttc gtctcgctcg aattcgacta tccgcccgcc gaactcgaaa ccgagatcgt
    11581 cgcgcgcgaa ggcggcattg acttgcaggc ggcgcgcgac ctcgtacaga tcggacacaa
    11641 gatcagaaat ctgcgcgggc acggcctcga agaaggagtc tcgacgcgcc ttctcatcta
    11701 cgccgcccag ctcatcgcgc gcggcatcgc gcccgttgcc gccgccgaag tcgccctcgc
    11761 ctcccccatc acggacgacc gcgaactcca acgcagcata cgcgagatca tcaccaccgt
    11821 catttgaatt tgaacgccat gaacgaagcc ttagccgcac actttctgga agatgcgctc
    11881 gcaatctttc gcaagtataa gaagctcgcc gaggatgcca ttgcgcagat cgacgacgag
    11941 gaattttttg cgcgcattga tgcggaggcg aacagcgttg cggtgatcgt gaagcatctg
    12001 gcggggaaca tgcgttcgcg ttggactgac tttttgacga cggacgggga gaaggcgacg
    12061 cgcgcccgcg atgcggagtt tgagatcgag ccggagacga cgcgtgcgga ggtcatgcgc
    12121 tggtgggaag agggttggag ttgcgtcttc acggcggtcg agccgcttga gccagcagac
    12181 cttgagcaca cggtgacgat caggggcgag ccgcacacgg tcgtcaaagc gatcaaccgg
    12241 cagttgaccc attacactta tcacacgggg cagatcgtgc tgctggcgaa gcatttcaaa
    12301 gcagagagtt ggagctccct gagcatcgcg cgcgggcaga cggaagcgtt caatcgatct
    12361 ctgcgcgaag gtgaatctac ggagaggctc tctgaaaagt aagcctgtgg ccgttcctcg
    12421 cagacctccc ggcgatcctc gaaacgcaat gagcaagcca gacgacaaag acgcgcccaa
    12481 cgcctcgaaa gcgaacgagg agacggctca gtcgcagcca ctcgcagatg ttctgccgga
    12541 acagttcgcg gaggtgcgga gcgcgagcga gcggcgcaac gtggcgggac tcgtgcgcga
    12601 gatcgggcaa ctgcccgccg accgcgcgcg cgccgcgctc gaagtgagcg cggggctggc
    12661 gagtttgagc ctgcgcgtcg ggctggaatt cctgcgcgcg gcgccgggcg cggcgcaggt
    12721 cttggaagcg gctgagttgc gcgagtgggg cgagatgggg cggcgtctgg cgatggccga
    12781 cgtggagacg ggcgcaaact tcttccgcgc gggcgtcgcg cagttgctcg ccgtgccgca
    12841 agcgacgcga ccgctgctgt ttcaagtctg cgcgcgccag ttgccgctct ctacttcgat
    12901 tgcgacggag actttcaggc tcgcgcccga actcgcacgc gccgccccgg acgaagccct
    12961 gctcgccacg acctttgaag tcgcgcttga gatcgcgcgc cgctccgcac gccacagcgc
    13021 agactttctc gccgcctcct tcgacgcctt cgcgcatctc aaaaactatg aacgggcggg
    13081 cgcgggcggg cgcgaaaacg aagttgcgcg cgccgcgctg catctcgccg gagtgttcgc
    13141 acagcgcgcg ggcggcgtcg ccgccgacgc ctggacgaac attcccgccg ccacctccgg
    13201 cctcgaagcc gcacacgcgc tcgcgctcct tcgccacacg gaaacttttc tcgaacgcgg
    13261 cggcgcggcg gcctcgcacg tcctcgtcgc gggcggcgaa gtgctcaggc tcgcgccgca
    13321 ggtctttgcc gattggacgg ccttgctcca cacggtcgcc gcgcagggca acgccccgct
    13381 cgtctcgctc gcgcgccaca gtccgctctt cttccgcgcc ctcccggcac gtgatgctcg
    13441 cggtgcggcg ttaacacagg gcacgtctga tctcgcgcgt cgtgtcatcg cggcgacgcg
    13501 cgccgtggcc gaggtagacg cggaggccgc tatctcctgt tttcgttccg cgcccaccgc
    13561 gctgcgcgcg acttcgctcg aacacttcga gcagtgggcg cgcgacggcc tcgcgctcaa
    13621 acgaaacgac gcgcgcacgc gccgcagtta ttacgcgctc gaaacccgcc agagcaacgc
    13681 cgcgctcaac gagggcggcg cgggactcgc gctcgaaacg gttgcgccga cgctgcgcct
    13741 ctacgtggaa ggtttgacgg ggcgcgccgt cgaagtggcc tcgctcgccg ccgtgcccga
    13801 cgaagcgcgc atcggcgacg ggcgcacgat ccacctgccc gccgccgtct ccgagtttgc
    13861 agacgacgag cgaaattttc gtctctacaa agtgctcgcc gcgcacgccg ccgggcagat
    13921 cgagttcgac acgcacgcac gcgccggaaa cgacacggcg gcgagcgcga gtttgcgcgc
    13981 cgccttcgct tcaatcgcag aactttacgc ggaagagaat agggacgcgc aggacgcctt
    14041 cgcgctcgac ggctatatca acgacccggc gcagagcgaa cccgccctcg cgcccgcaga
    14101 ggaagcgcgc cgcgcgcgaa acagccggag gcggatgccg aaggagtcgg actatcgcgc
    14161 cgtgctcgcg ctctttcccc tcccgacgct cgccgcgcgc atcttcggca cgctcgaaaa
    14221 cggtcgcatt gatcgacgcc tgcgccacgc ctatcgcggc ctcgcacgcg atctcgatct
    14281 cgtgcgcaca cacctgcgcg cgagccgccc gcgcatcgca accatccccc tcacgctcgt
    14341 ccccttcgag ttgctcttcc agatcacgct ctgcggcggc gcgctcgacg acgcgcgctc
    14401 cttctacggc cagatcgtct ccgaactcga atcaatcgtc gccggttacc tgaacggcgc
    14461 ggaggcttcc gtcgcggaca ccctgatggc gacgaaccgc gtctacacgc tctttcagtc
    14521 cgtcacgccg caggccaccg agcagcagga acagcaatcg gagacgcagg acggaggcgc
    14581 gagcggtgcg gaatctttgc tcgaagatgc gagcgcgcga aagccgcgcg aacccgaagg
    14641 ggagccgcga aacgatccgc cgcccaatga tgcgcgcgaa ctcttcaacg cgtgggcgaa
    14701 gtccaactcg tccgaagccg acgccgccga gcagcttttc ggcgcggagc gttggacgca
    14761 gtcgcagtcg ccggagcagg atttggagcc gggcgaagtg gcttacagtt acgacgagtg
    14821 ggatcgtgaa ctcgcggaca cgcgcgtcaa ctggtgtcgc gtcgtagaga aacgggtccg
    14881 gcgcggcgac cgctcctttg tcgaactcac gcgcacgcgt tatcgcggcg tcatctcttc
    14941 gatcaagcat caattccaga tgatgcgccc cgaaagtttg ctgcgcatca agaacgaact
    15001 cgacggcgac gattacgatt tggatgccgt gatcaattac gtcgccgacc gtcgcgccga
    15061 cgggcgacaa tccgaacgcc tctacacgaa acgcctgcgc cgcgagcgcg acgtggccgt
    15121 ctcgtttctg ctcgaccagt cttcctcgac ggcgcgcacc atcggtcgtc atcccttgca
    15181 gccctacacg catcccggtc gccgcatcat cgagatcgaa aaggaagggc ttgtcctgat
    15241 gagcgaagcc ctcgaagccg tgggcgacgc ttacgccatc tacggtttca cgtccgaagg
    15301 gcggcgcaac gtgaaatttt acgtcgtcaa agattttcac gaacgccact ccgaagagat
    15361 cgagcggcgc atcggcggca tcaactatca acacaacacg cggctcggcg cggctgtgcg
    15421 ccatgccgcc gcgcgcctgc gtgtgcagga agcgcgcacg cgtctcctca tcgtgctctc
    15481 ggacgggcgg ccttacgatc acgattacgg cgacgcgcgc tacgcacgcg aagacacgcg
    15541 cgaagccctg cgacaggctc gcgtcgcggg cgtcacgcct ttctgcatca ccatcgaccg
    15601 cgactccgag gccgaactcc gcgacctcta cggcgaggtc ggctacacga tcatagacga
    15661 cgtgctcagc ctgccggagc ggatgcccgc catctatcgc cgtctaacga cgtgaacggg
    15721 gcgagagcgc gggaagaagg ccgggtcgca gtttagaggg gcgaggcggc gagttgtcgc
    15781 tgccccgctc gcgtaatttt cggagacgaa agttccctct attcgtctct gacgcacata
    15841 agttttcttc ccgttatagt ttcgtcaaat agttcctcta aaagagagcc taaaattagg
    15901 cgcaagaatg gcgaacgtct gtcgttcttg aacttgaaaa cgaaactgtt cgcgcgccgc
    15961 cgggctaaaa gaacggtcgg gcaccagcgc gcgattattg accactaggg attcggctaa
    16021 cattaggaga aacttcaatg cgacgaactt tagctctttt gagcctgctg gctctggcga
    16081 ccttcggggc tgcctgcgct gcttcggaca atgcaaacac aggggccaat gccaataacg
    16141 ccaacaatat gaacgccacg accggaacga cgggtacggg cacgacgggt acgggcgcga
    16201 cgaccggaac cggcacgacc ggaacgaccg gaaccgccga caacatgaac acgaccggcg
    16261 gcacgaccgg cggcacaacg ggcggcacaa cgggcaccgg cggcaacacc aatacgggtg
    16321 ggaacacgaa catgggcggc aacgccaatc gtgggaacac gaacgcgggc aacggtaaca
    16381 ccggcggcgg caacggcaac atgcgtaccg gcaacacgaa caccgggacg gggacgggcg
    16441 cgaaccgcaa cactccctag tctctacgcg aaagtttttt tcaagagtct ggataaaaaa
    16501 agggcaaggg cgcggtcgga gtttgaccgc gcccttgccc tttccgggcg aggtgggatc
    16561 gcttcacgtc gaatcaacaa gtccagagtc tcaagtcaaa gacggtttga gactctggac
    16621 ttttccctgt tgtcccggct tcgctacttt ccggcgaagc gaaaggtgaa gcccgccgtc
    16681 acgccgaagg ggtagccggg cgtgccatga atccgcgcga cgaccgggtc gccgggtctg
    16741 aggcgcgact cgaaatagtt ctgcgtctcg aagtaacgct tgttcgtcag attgtctatc
    16801 gagagattga aatccacgaa acgccggatg cgcttgcgga ggctgaaatc gagcacgtcg
    16861 aggccggagg cgcgaatggt ggcgtcctct ccgtcgagac ggtagctgcc ggtgtgcctg
    16921 taattgagcg cggcgtcgaa gccgcgatag ccggagaggg tgagcgcggc gttggcgacc
    16981 gtgtgcggcg cgctgtccac gtagacgcgc gggcgcgtgg cgcgaaaaaa tgcgttgctc
    17041 acctgcgtca ggccgccgtt gaaggcgagg tgcttcgtca gttgcgcgga ggtcttgaac
    17101 tcgtaaccgt aggaacggct tgcgcccctg aactcgaagc tgccgtcgtc cgggatgtag
    17161 acctgttcgt tcgagcggtc gatgaggaag aggtctgtga cgagcgagaa ccgcttgaac
    17221 ttgtgcgacg tgccgagttg gtaaaaatcg gtggtggaga ctttcggggc ggaaggattg
    17281 ccgacgacgc cgcgcgcgtc ctgactcgaa atgccgcgcc cgtagttgaa atagaaggtg
    17341 gccggaaagt tgtcggcagg attgtaggcc gcgctgaact tgggctggaa gcgcgcggcg
    17401 gactgcgcgc cagagagcgc gggagcggcg cggtcgtcca ccttgaagcg gaaatagtcg
    17461 tagcgcacgc ccgcctcgat gtggaggtgg ccgtgcagga ggtcgatgcc gtgttgcacg
    17521 tagccgctcg cgttcgtgac gcgcgcccgc gcgtcggtca acgggcgcgt ggtcacgccg
    17581 gggctgacgg cttcgcccac ggcaatcgga ctgcgcgcga cggtcgggta gagtccgacg
    17641 ttgatctgat tgtcgtgata gtttgcgccg acggtgatga gcgcgagatg cttgaaaagt
    17701 ttataggggc gcaggtattg gaggttcgcg ccttcctgta gacgcgaatc gtgctgctga
    17761 atctcgtcgc cgttcacttc gtctgtgaga aagaaggtga agttggaata gagatcgaag
    17821 agcgagcgcg agacgaaggc gtcggccttg aaggtcgcgc ccgatgcgga ctcgttgcgg
    17881 aaatagaggc cgaccatccc cgcatgcacg cgcccgccgt tgtccgggtc gatgaggccg
    17941 aagcggtcga gtcggcccgc gaacacctcg tc
//
LOCUS       DASWNS010000018        13054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_441621, whole genome shotgun sequence.
ACCESSION   DASWNS010000018 DASWNS010000000
VERSION     DASWNS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441621"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            556..1059
                     /locus_tag="VGB76_00425"
     CDS             556..1059
                     /locus_tag="VGB76_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097388.1"
                     /translation="MYRPNFCAECGEPVVRLRWRLWTSRRFCATCDKRFRKSRLLWRV
                     AACLALTGVGFTGGRALRQAPPPLVIERGAFNSPASSSQTPATARQVSANANAANVNA
                     GASLYGADGTTRERPTEAGEVVSICGARTKRGTPCSRRVRGTGRCWQHRGRAAMLPAA
                     KLIVREE"
     gene            1283..4582
                     /locus_tag="VGB76_00430"
     CDS             1283..4582
                     /locus_tag="VGB76_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0097389.1"
                     /translation="MMKKIYLSAIAFLFLCSYVATAKEARLVRYPHYHQGRITFGYLG
                     DIWTADETGQNIQRLTVHKARDVYPRFSPDGKWIAFSSDRNGNLDVFIMPSSGGTVKQ
                     LTNHSSDDSVLNWTPDGTRVLFSANRGEDFSARLYTVSTDGGMPANAGTDMGVQGSFS
                     PDGRRLAYNQKAQAYWRKFYRGSMQSDVMVMDASTKKFTNLTDFEGLDSWPMWARDGH
                     IYFVSDREGGGLTNIWRVSEAGGKAAQVTNFKSGDVRWPAISADGKTIVFEHNFGISK
                     FDVGSKRVTPISLDIAAETQESLSEVRDYASQVDDYALAPNSRRIAFSIHGEVFTAPV
                     EEGDLRQLTESPFRDRNVEYSPDGKWIAFVSDRSGREELYVVAVDGAGEAQKLTDLDE
                     LKYGYAWSPDSTQISYSASDNKLRVVNVATKASKEISSSRYGQIGFGSWSPDGKWLAY
                     SKPDFTRTSDVYIAPSTGGEERRVTFDSFTDTGSRFSPDGKKLYFQRVDQSSFSSLTS
                     VQIYSITLEKLERDPDDAEERAEQEAAEAARQQPSGEASAGGAARPPMPRPTPKPVNI
                     DWAGLKRRTRQLTRMPFAVTSYTIAPDSKSIVFITSEPAGAAGNLPVIYSIQEDGKRL
                     TRITSGTPPTDGAGGGGGGGGLGSGLSNLSVSRDNRTLFFQEGQFVYSVPMPPPVPAG
                     APASSAPPATTASAAQRRRINFNAKVRIDRPAEWAEMFDDAWRTMKYRFYDAKMHGTD
                     WDAMRAKYRPLVEYVGDRQELLNIVNEMIGELNASHTGAAPPPGGSQPGAAQTGHLGL
                     ELEPDAAAGRYRVKHIYEDGPSDKDWVKVSVGDYLIAIDGKPVRAGDEYWQHLNYRLN
                     RKVAVTFNNKAAEEGAWRTRIEPTSMGNYSQLRYERWVKERRDLVSKLSNGRVGYLHI
                     QAMNQPSLRKFEKELREYRDKEAMIIDQRWNGGGNIEQELLGILVQRQYQVWQPRGTE
                     PTTRPLAGFFGPKVVLQNWRSSSNAEMFPAGFRALGLGKVIGTPTMGAVIGTGSYSLI
                     DGSTVRTPGIGVYLADKGQTNMENNGVRPDILVDNTPEETLAGRDRQIEVAVEELLKQ
                     LKSGNNSVANK"
     gene            4977..6959
                     /locus_tag="VGB76_00435"
     CDS             4977..6959
                     /locus_tag="VGB76_00435"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0097390.1"
                     /translation="MIEAKNMAVPIQGDAYDSSTIAPVADWRALSELEEHFVQFYEAD
                     EFLLDSLGGFIGAGLAAGDACIVVATEAHREALEERLQATGLDTAAARAGGQYVVLDA
                     SEILSKFMSGGQPDPVRFAEVVGSIVGRAAEGRERVRIFGEMVALLWTEENYHAAIRL
                     EELWNELQRTHSFSLFCAYPMQGFGGELLASGLADVCTTHARVIPAESYTALADPDER
                     LRKIITLQQKARTLEAEIAERKEAEERLRISLAQEQLARAEAEHASRLKDEFLATVSH
                     ELRTPLTAIIGWSYMLRHGKLSEANAVRALETIERNAKSQAQIVEDILDVSRVITGKL
                     RLNIEAVDVASVINAAIDSAQLAAESKHIQLQVTLDPSARHVSGDASRLQQVVWNLLS
                     NAIKFTPKGGRVEVRLERKEANAQLRVSDTGCGLHPEFLPYIFDRFRQADGSNTRCHG
                     GLGLGLSIVRHLVELHGGTVRADSPGAGGGATFTVELPLAPTRSSVRNGETGGHYSRS
                     NEEALSAHAGSLPSLEGVRVLLVDDAPDTLNTLNAILTEHNATVQIAASAAAALEVLA
                     RYKPSVLVSGLTMPCADGYTLIGRIRAQETESGDEPVPAIALTAYVRVEDRVRALAAG
                     FNMFVPKPVDPNELIVAILSLAEPRTTGLLHSDSEM"
     gene            complement(7060..8436)
                     /locus_tag="VGB76_00440"
     CDS             complement(7060..8436)
                     /locus_tag="VGB76_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0097391.1"
                     /translation="MKLKSLLRFVAVARGLAVALAFSMLLPVAATAQQASSTMGPQFM
                     SGTPGTFAIRNARIFTVSGAEIENGTVVISDGRIAAVGASVAVPAGAQEIDARGLSVY
                     PGMIDLGTAMGLIEIPQGANATVDVAETGDMNPNAQAFYGINPHSAHVAVTRVNGVTS
                     VLSLPAGGIISGQAAFINLLGSSPQEMAVLPAAALVVTYPRAGGGGGFGGFFGQPQAN
                     PTEAVAARDRQVEQLRKMLRDAEAYGRAMDAAERDRALPRPDADVVLASLVPYVRGER
                     PVVFRADREPEMRAVIRFAEEMKLKPIILGGTDAWKMAAFLKERNVPVILSGVFNLPS
                     REDDFYDVMYENPSKLQQAGVRFALSTGDEGAEVRDLPYRAGMAAAFGLSRAEALKSV
                     TLYPAQIMGVGDRLGSIETGKIANLVVTDGDLLEARTHIRHLFINGRQVPLNSRHTEL
                     YDQFKNRK"
     gene            complement(8433..9917)
                     /locus_tag="VGB76_00445"
     CDS             complement(8433..9917)
                     /locus_tag="VGB76_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0097392.1"
                     /translation="MTKLISLFIAYAVLVPVWGATAHAQQRRTQPQQPGAARTGADAA
                     AGGETLIRNATILTISRGTLQNSDILIRNGKIAAVGQNLKAPERARVIDATGKYVMPG
                     IIDPHSHSMSDAINEGTLSVTSMVRIRDMLNPTAPNLYRELAGGTTTLNLLHGSANTI
                     GGQNTVVKIKYGRPVGEFIFPGAPPGIKFALGENVKRASSTVLPGQTRRYPATRMGQE
                     EVLRDAFTRARNYKRAWDDHRAATARGDRNLIPPRRDLQLEPLVEVLEGKRLVHAHCY
                     RADEIIMMLNVAQEFGFRVKTLQHVLEGYKVAKEIAAHGAGASPFADSWGYKIEAYDA
                     IPYNVAVMMRAGVLVSINSDSDERARRLNIDAAKMMKYGDLSEEEALRLITINPAIQL
                     GIQDRVGSIDVGKDADLVIWNGHPFSVYSRVERTFIDGDIFFDREQDLARRAELQRER
                     EELLKAEVNRPPSQGATPPSAPRTRPSNHHDHDDVDNGEGDNHR"
     gene            complement(10130..11899)
                     /locus_tag="VGB76_00450"
     CDS             complement(10130..11899)
                     /locus_tag="VGB76_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0097393.1"
                     /translation="MRKDPARTDGRRRLAPVISLSLVVALLAVMLAAPPRAAALLFFT
                     QRSAIDTYAITNARIVPVSGAEIARGTVVIRNGLIAAVGANVAAPADARIIDGAGLTI
                     YPGLIDANTNLGMPAAPTRPAGGGGGVAAFLAAQQQPTPAVVSPNSSQPVGLQPEILA
                     SDIVRPGGEQLDAARNAGLTAALTAPREGIFMGQSAFVNLSGETPQQMIVRAPVALHV
                     GFTPLRTGTYPGSLLGVFAAIRQMMLDAARYREANAIYEKSPRGVRRPDQDKSLDALL
                     PVLARQMPVVMYADRQREIERALDLAQEFNLRLIIAGGAESWRVSERLAAAKVPVLLS
                     LNYPRRTTAESPEADPDPIRVLRERVDAPKTAGRLAAAGVRFAFQSGGLANMSDYLAN
                     VSKSVENGLARDEAVRALTLRAAEILGVEAQLGTIETGKIANLTIVRGDLFDKNRRIT
                     HVFIDGRPVDLKPAPATPAGAPGGAPRTASLASGTWTLRVQLEGQPERSVTLTLQQEG
                     ERLSGSIQGDFGSSQIANASVGASGDIKFTVPITTGTQTTEATFNGTISGNEMRGTVT
                     TVGTGTGTFNGTRAAGGATPPGQ"
     gene            complement(12294..12548)
                     /locus_tag="VGB76_00455"
     CDS             complement(12294..12548)
                     /locus_tag="VGB76_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097394.1"
                     /translation="MKKLATLVALAALSAGCTATDNANTNAGARNGNAAIVVPNNANN
                     ANAANANAANANANASNANANASNANTKAGGAAHNANANH"
BASE COUNT         2448 a         4007 c         4217 g         2382 t
ORIGIN      
        1 gattgcaata aagtttcaga cccatcggcg taaccaaacc agcggcggcg ggagaatgac
       61 gggcggggcg cacgacgctt ctgaagcgcg acgacgaccg gagcgcaatt ctcagtcatt
      121 cgggagtgac gaagaatata gcacgtttgt tttgcacatg caacactcgc cagcgcgtga
      181 aacgaaggcg acggagaacg aggcacaagt ttcaccccgg aacagcccgt gcctcaagca
      241 gaaatagcat cgcactcaaa cagaaatagc ttgtgcctcg aacagaaata gcttgcgctc
      301 aagcagaaat agcttgcgct ccggccttcg cttccgtcta taatccgcgt gcgtagcagc
      361 aaactgatcc gttgcggcag agcagccgac ggcagacgcg tttcctcccg ccttacagga
      421 agcgttcgct gcgccctttt ttaagcgacg cgctcgataa actcaccacc acagcaacca
      481 tagcggcagc ggcgaagcag cgacagcggc agcaccggcg acgcgcgccc acacgtctct
      541 ggagaactcc cgaagatgta caggccaaac ttctgcgcgg agtgcggcga gcctgttgtt
      601 cgcctgcgct ggcgtttgtg gacgagtcgc cgtttttgcg cgacgtgcga caaaaggttt
      661 cgtaaaagcc gcctgctgtg gcgggtcgcc gcgtgcctcg cgctcacggg cgtaggcttc
      721 accggcggtc gcgcgttgcg tcaagcaccg ccgccgctcg tcatcgaacg cggcgcgttt
      781 aactctccgg cctcctcgtc gcaaactccg gcgacggcca gacaagtttc cgcgaacgcc
      841 aacgccgcga acgtcaacgc gggcgcgagc ctctacggcg cggacggcac cacgcgggaa
      901 cgacccacgg aggccggaga ggtcgtctcc atctgcggcg cgcgcacgaa acgcggcacg
      961 ccctgttcgc gccgcgtgcg cggcacgggt cgctgctggc agcatagagg acgtgcggcg
     1021 atgctgcccg ccgcgaagtt gatcgtgcgt gaagagtgag agggggaaaa aacttttctc
     1081 ttcgcgcccc gtaatatttg gcgttcgcag tgcgttttaa ctacaatgca tgagctaatc
     1141 ctgctaaagc gtcctcgctg gggcgcaaaa aagaatttcc tgtccgcatc aagagctaaa
     1201 agtcgtcgtt gctgctcgaa gcagcccgtc ggggcgtcgc tcgtgagggc ggccttaact
     1261 ctctgaaaaa ctgtctaaaa aaatgatgaa gaaaatctac ctatcggcaa tcgcttttct
     1321 attcctctgc tcctacgtcg ccacggcgaa agaagcaagg ctcgtgcgtt acccgcatta
     1381 tcatcagggg cgcatcacgt tcggttatct cggcgacatc tggacggcgg atgagacggg
     1441 gcagaacatc cagcgtctca ccgtgcataa agcgcgcgac gtgtacccgc gcttttcgcc
     1501 cgacggcaaa tggatcgctt tttcgagcga ccgcaacggc aacctcgatg tctttatcat
     1561 gccttcgagc ggcggcacgg tgaagcagtt gaccaaccac tcttccgacg attccgtcct
     1621 caactggacg cccgacggca cgcgcgttct cttctccgcg aatcgcggcg aagacttctc
     1681 cgcgcgcctc tacacggttt cgaccgacgg cgggatgccc gcgaacgcag gcacggacat
     1741 gggcgtgcaa ggctcctttt cgcccgacgg gcggcggctc gcctacaacc agaaagcgca
     1801 ggcgtactgg cggaagttct atcgcggctc gatgcaatcg gacgtgatgg tgatggatgc
     1861 gtccacgaaa aagttcacga acctgaccga cttcgaaggg ctggactcgt ggccgatgtg
     1921 ggcgcgcgac ggccacatct atttcgtctc cgaccgcgaa ggcggcggac tcaccaacat
     1981 ctggcgcgtc tcggaagcgg gaggcaaggc cgcgcaggtc acgaatttca agagcggcga
     2041 cgtgcgctgg cctgccatca gcgccgacgg caaaaccatc gtcttcgagc acaacttcgg
     2101 catcagcaaa ttcgacgtgg gatcaaaacg cgtcacgccg atctcgctcg acatcgccgc
     2161 cgagacgcag gagagcctga gcgaagtgcg cgactacgct tcgcaggttg acgattacgc
     2221 gctcgcgccg aattcgcgcc gcatcgcctt ctccattcac ggcgaagtgt tcaccgcgcc
     2281 cgtcgaagag ggcgacctgc gccaactgac cgaaagccct ttccgcgacc gtaacgtcga
     2341 atattcgccc gacggcaagt ggatcgcttt cgtctccgac cgcagcgggc gcgaagaact
     2401 ctacgtcgtc gccgtggacg gcgcgggcga ggcgcagaag ctgacagacc tcgacgaact
     2461 caagtacggc tacgcctggt cgcccgattc cacgcaaatc tcctattcgg cttccgacaa
     2521 caaactgcgc gtcgtcaacg tcgccaccaa agcgtcgaag gagatcagtt cctctcgcta
     2581 cgggcagatc ggcttcggct cctggtcgcc cgacggcaag tggctcgcct attccaaacc
     2641 cgacttcacg cgcacgagcg atgtctacat cgcgccttcc accggcggcg aagagcgcag
     2701 agtgaccttc gattccttca ccgacacagg ctcgcgcttc tcgcccgacg gcaagaaact
     2761 ctatttccag cgcgtagacc agagcagctt cagttcgttg acctccgtgc aaatctattc
     2821 gatcacgctc gaaaaattgg agcgcgaccc ggacgacgcg gaggagcgcg ccgaacagga
     2881 ggccgccgaa gccgcgcgcc aacaaccttc cggcgaagcg tccgcaggcg gcgcggcgcg
     2941 tccgcccatg ccccgcccga cgccgaagcc tgtcaatatt gactgggcgg gtctgaaacg
     3001 ccgcacgcgg caactgacgc gcatgccttt cgccgtcacg agctacacca tcgcgcccga
     3061 cagcaaatcc atcgtcttta tcacgagcga accggcgggc gcggcgggga acctccccgt
     3121 catctattcg attcaggaag acggcaagcg actcacgcgc atcacctccg gcacgccgcc
     3181 gaccgacggc gcgggcggtg gcggcggtgg cggcggtttg ggcagcggcc tctccaacct
     3241 gagcgtctcg cgcgataacc gcacgctctt ctttcaggag gggcagttcg tctattccgt
     3301 gccgatgccg ccgcccgtgc ctgcgggcgc acctgcatcg agcgcgccgc ccgcgacgac
     3361 ggcaagtgcg gcgcaacgtc gccgcatcaa cttcaacgcg aaggtcagaa ttgatcgtcc
     3421 cgccgagtgg gcggagatgt tcgacgatgc ctggcgcacg atgaaatatc gtttctacga
     3481 cgcgaagatg cacggcacgg attgggacgc gatgcgcgcc aagtaccgcc cgctcgtcga
     3541 atacgtcggc gaccggcagg aactcctgaa catcgtcaac gagatgatcg gcgaactcaa
     3601 cgcttcgcac acgggagccg cgccgccgcc gggcggaagc cagccgggcg cagcccagac
     3661 cgggcacctc ggcctcgaac tcgaacccga tgcggcggcg ggtcgttatc gcgtcaagca
     3721 catctacgaa gacggccctt cggacaagga ttgggtcaaa gtctcggtcg gcgattacct
     3781 gatcgccatt gacggcaaac ccgtgcgcgc gggcgacgaa tactggcagc atctcaacta
     3841 ccggctcaat cgcaaggtcg ccgtcacctt caacaacaag gctgcggaag agggcgcgtg
     3901 gcgcacccgc atcgaaccga cttcgatggg caactacagc cagcttcgct acgaacgctg
     3961 ggtcaaagag cggcgcgact tggtatcgaa actctcgaac gggcgcgtcg gctacctgca
     4021 catacaggcg atgaaccaac cctctctgcg caagtttgag aaggaactgc gcgagtatcg
     4081 agacaaggag gcgatgatca tcgaccagcg ttggaacggc ggcggcaaca tcgaacagga
     4141 gttgctcggc attctcgtcc aacgccagta tcaagtctgg cagccgcgcg gcaccgagcc
     4201 gacgacgcga ccgctggcag gcttcttcgg tcccaaagtc gtcctccaga actggcgttc
     4261 gtcttcaaac gcggagatgt ttcccgcagg cttccgcgcg ctcggactcg gcaaagtgat
     4321 cggcacgccg acgatgggcg cggtgatcgg cacgggcagc tactctttga tcgacggctc
     4381 gaccgtgcgc acgcccggca tcggcgtcta tctcgccgac aaggggcaga ccaacatgga
     4441 gaacaacggg gtgcgccccg acattctcgt ggacaacacg cccgaagaga ctcttgccgg
     4501 gcgcgaccgc cagatcgaag tcgccgtcga agaactgctc aagcaactca agagcggcaa
     4561 caactctgtg gcgaacaaat agccgatagg cgggaagcga acagttaaaa ggccacggcg
     4621 cagacggaag gctcgaatga tccgtctgcg tcgtggactt ttcttttgcg cgggggcatc
     4681 ggtttcaggg cgcggcgcag cccggatcgc ccgcctctgg ctttcgggcg ggaagagagc
     4741 taaaatgctc cggcgcggac atcccctcct cgcgagtctg cggttgaatt cggactactg
     4801 cgcgccagta gtcttttgac tgaaaccgga acgccgccaa tatcccctta aggtcaatac
     4861 gcccggcagc cgtctgtcaa atgaccgagc cggggaattt ccaaaacctc ggagtgtgag
     4921 aatgcggagg cgcgattccg gcgcaccccg tttattgatt cgcggagaga ctctacttga
     4981 tcgaagctaa aaatatggcc gtgccaattc aaggcgacgc ttacgacagc agcaccatcg
     5041 cgcccgtcgc ggattggcgc gcgttgagtg aacttgaaga gcacttcgta cagttttatg
     5101 aggccgacga atttctactg gattcgctcg gcggcttcat cggcgcggga ctcgctgccg
     5161 gagatgcctg catcgtggtg gcaacggagg cgcaccgcga agctctcgaa gagcgtttgc
     5221 aggcgaccgg gttggatacg gcggcggcgc gcgcaggcgg ccagtatgtc gtgttggatg
     5281 cgtcggagat tttgtcgaag ttcatgtccg gagggcagcc cgatccggtt cgcttcgcgg
     5341 aagttgtggg gagcatcgtc ggacgcgcag cggaaggccg tgagcgggtg cgaatcttcg
     5401 gggagatggt cgcgctcctg tggacggaag agaattacca cgccgccatc cgtctcgaag
     5461 aactctggaa cgaactgcaa cggacgcact cgttctcgct cttctgcgct tatcccatgc
     5521 agggcttcgg cggggaactg ctcgccagcg gactcgccga cgtgtgcacg acgcacgcgc
     5581 gcgtgattcc cgcagagagc tacaccgcgc tcgctgaccc ggacgaacgc ctacgcaaga
     5641 tcatcacgct gcaacagaaa gcgaggacgc tcgaagctga gatcgcggaa cgcaaggagg
     5701 ccgaagagcg tctgcgcatc tcgctcgcac aggagcaact cgcacgcgca gaggccgagc
     5761 acgccagtcg tctgaaagat gaatttctcg ccaccgtctc gcacgaacta cgcacgccgc
     5821 tcacggccat catcggctgg tcgtacatgc ttcgacacgg caagctgagc gaggccaacg
     5881 cggtgcgcgc gcttgaaacc atcgaacgca acgccaagtc gcaggcgcag atcgtcgaag
     5941 acattctcga cgtgtcgcgc gtcatcacgg gcaaacttcg tctcaacatc gaagcggttg
     6001 acgtggcctc cgtgatcaac gccgcgattg attccgcgca gttggcggcg gagtcgaagc
     6061 acatccaact gcaagtaacg ctcgatcctt ccgcccgcca cgtctcgggc gatgcgagcc
     6121 gtttgcagca ggtcgtctgg aacctgctct cgaacgcgat caagttcacg ccgaagggcg
     6181 ggcgcgtcga agtgcgtctg gagcgcaaag aggcaaacgc gcaactgaga gtgagcgaca
     6241 cgggctgcgg cctccacccg gaatttctgc cctacatctt cgaccgcttc cgtcaggccg
     6301 acggctctaa cacacgctgt cacggcgggc tgggcttagg gctttcgata gtcaggcatc
     6361 tcgtcgaact ccacggcggc accgtccggg cggacagccc cggcgcgggc ggcggcgcga
     6421 ctttcaccgt cgaactcccg ctcgccccga cgcgaagcag cgtgcgaaac ggcgagacgg
     6481 gcggacacta ttcacggagc aatgaagaag ccctgagcgc gcacgccgga agcctgcctt
     6541 cgctcgaagg cgtgcgcgtg ctcctcgtgg acgacgcccc tgacacgctc aacacactca
     6601 acgcgatcct gaccgagcac aacgcgacgg tgcaaatcgc cgcttccgct gccgccgcgc
     6661 tcgaagtgct cgcgcgctac aagccctccg tgctcgtctc cggcctgacc atgccctgcg
     6721 cggacggcta cacgctcatc ggcaggatca gagcacagga gacggagagc ggcgacgagc
     6781 cggttcccgc catcgccctg accgcttatg tcagggttga ggatcgcgtg cgcgcactcg
     6841 cggcgggctt caatatgttc gtcccgaagc cggttgaccc gaacgagttg atcgtagcca
     6901 tcctgagtct cgccgaaccg cgcacgacgg gcctgctcca ctccgactcg gagatgtagc
     6961 gaactgcgcg cggcacacac cgacgatgaa aaaggcaggc gggactttcg agtcccgcct
     7021 gccttcttcg ttatgcgacg tgcgcttttt ttgtcttccc tatttacggt ttttgaactg
     7081 gtcgtacaac tccgtgtggc gactgttgag cggcacctgt cgcccgttga tgaagaggtg
     7141 acggatgtgc gtgcgtgctt cgagcaggtc gccgtccgtg acgacgaggt tggcgatctt
     7201 gcccgtctcg attgagccta agcgatcccc gacgcccatg atctgcgccg ggtagagcgt
     7261 gacggatttg agcgcctcgg cgcgggagag tccgaaggcg gcggccattc cggcgcgata
     7321 tggcaggtcg cgcacctccg cgccttcgtc gccggtcgag agcgcgaagc gcacgcccgc
     7381 ctgttgaagt ttggaagggt tctcgtacat cacgtcgtaa aagtcgtctt cgcggctcgg
     7441 caggttgaag acgccgctta agatgacggg cacgttgcgc tctttgagaa acgcggccat
     7501 cttccaggcg tccgtgccgc cgaggatgat gggcttgagt ttcatctctt ccgcaaagcg
     7561 gatgacggcg cgcatctccg gctcgcggtc ggcgcgaaag acgacgggac gctcgccgcg
     7621 cacgtaaggc acgagcgaag cgagcacgac atcggcatcg ggacgcggaa gtgcgcggtc
     7681 gcgctccgcc gcgtccatcg cgcgcccgta ggcttccgca tcgcggagca tcttgcgcag
     7741 ttgctcgacc tgccggtcgc gcgccgcgac ggcctccgtc gggttagctt gcggctgccc
     7801 gaagaaaccg ccgaagcctc cgccaccgcc cgcgcgcggg taggtgacga cgagcgcggc
     7861 ggcgggcagg acggccatct cctgcgggct tgagccgagc aggttgatga aggcggcctg
     7921 ccccgagatg atcccgcccg caggcagact caagacgctc gtgacgccgt tcacgcgcgt
     7981 cacagcgacg tgcgcgctgt gcgggttgat gccgtagaag gcttgcgcgt tcggattcat
     8041 gtcgcccgtt tcggccacgt caacggtcgc gttcgcgccc tgcggaatct ctatgagtcc
     8101 catcgccgtg ccgaggtcga tcatgccggg atagacgctc aacccgcgcg catcgatctc
     8161 ctgcgctccc gcaggcaccg cgacgctcgc gccgacggcg gcgatcctgc cgtcgctgat
     8221 gacgacggtg ccgttctcga tctctgcgcc ggagacggta aagatgcgcg cgttgcggat
     8281 ggcaaacgtg cccggcgtgc cggacatgaa ctggggtccc atcgtgctcg acgcctgctg
     8341 cgccgttgcc gcgacgggca ggagcatcga aaacgcgagc gcgacggcca gaccgcgagc
     8401 gacggcgacg aatcttagta gtgatttcag cttcatcggt gattatctcc ttccccgttg
     8461 tcaacgtcgt cgtggtcgtg atgattcgag gggcgcgttc gaggtgcgct cggcggcgtt
     8521 gcgccctgac tcggcgggcg gttcacttcg gccttcaata actcctcgcg ctcgcgttgc
     8581 agttcggcgc ggcgcgctaa gtcctgctcg cggtcgaaga agatgtcgcc gtcgataaac
     8641 gtgcgttcga cgcgcgagta gacgctgaac gggtggccgt tccagatgac gaggtctgca
     8701 tctttgccca cgtcaatcga gccgacgcga tcctgtatgc cgagttgaat ggccggattg
     8761 atggtgatga ggcgcaacgc ttcctcttcc gagaggtcgc cgtatttcat catcttcgcg
     8821 gcatcaatgt tcagacgacg cgcgcgctcg tccgagtcgg agttgatgga gacgagcact
     8881 ccggcgcgca tcatcacggc gacgttatag ggaatcgcat cgtaggcttc gatcttgtag
     8941 ccccaactgt ctgcgaaggg cgacgcgccc gcgccgtgcg ccgcgatctc tttcgccacc
     9001 ttgtagcctt cgagcacgtg ctggagcgtc ttgacgcgga agccgaactc ctgcgcgacg
     9061 ttgagcatca tgatgatctc gtcggcgcga taacagtgcg cgtggacgag tcgtttgcct
     9121 tcgagcactt cgacgagcgg ttcgagttgc aggtcgcggc gcggcgggat caaattcctg
     9181 tcgccgcgtg ctgtggcggc gcggtggtcg tcccaggcgc gtttgtagtt gcgtgcgcgg
     9241 gtgaaggcat cgcgcaaaac ttcttcctgt cccatgcgcg tcgcgggata gcggcgcgtc
     9301 tggccgggca ggacggtgga actcgcgcgc ttgacgtttt cgccgagcgc aaacttgatg
     9361 cccggaggcg cgccgggaaa gatgaactcc ccgaccgggc ggccatactt gatcttgacc
     9421 acggtgttct gcccgccgat ggtgttggcc gagccgtgca ggagattgag agtggtcgtg
     9481 ccgcccgcga gttcgcggta gaggttgggc gcagtcgggt tcagcatgtc gcggatgcgc
     9541 accatcgaag tgacggagag cgtgccttcg ttgatcgcat cggacatcga atgcgagtgc
     9601 gggtcgatga tgccgggcat gacgtacttg cccgtcgcat cgatcacgcg cgcacgctcc
     9661 ggcgctttca ggttctggcc gacggctgcg atcttgccgt tgcgaatcag gatgtcggaa
     9721 ttttgcagag tgccgcgcga gatggtgagg atcgtcgcat tgcgaatcag cgtctcccct
     9781 ccggcggcgg cgtctgcccc ggttctggcc gcgcccggtt gctgcggctg cgtgcgccgc
     9841 tgctgcgcgt gcgccgttgc gccccacacc gggacgagca cggcgtaagc gatgaaaagt
     9901 gaaataagtt ttgtcatacc aagctccatg aaacagtgac gagtgacaag tgacgagtag
     9961 aaaacagttc tgagccgtaa gcgataagcg acgggtgacg agcggcgagt gacaagttaa
    10021 aaagccgtga atcgtagatc gtgaatcgtg aatagaagag atcagtttta gagctattca
    10081 cgatctacga ttcacgatct actgtcttca tctcgtcact cgtcactcgt cactgtcccg
    10141 gcggcgttgc gccgcctgcg gcgcgcgtgc cgttgaaggt gcctgtgccg gtgccgacgg
    10201 tcgtgacggt gccgcgcatc tcgttgccgc tgatcgtgcc gttgaaggtg gcttcggtgg
    10261 tctgcgtgcc cgtcgtgatg gggacggtga acttgatgtc gccgcttgcc ccgacggatg
    10321 cgttagcgat ctgcgacgag ccgaagtcgc cttgaatcga accgctcagg cgttcgccct
    10381 cctgttgcag cgtgagcgtg acggagcgtt ccggctggcc ttcgagctgc acgcgcagcg
    10441 tccacgtgcc ggacgcgagc gaggcggtgc gcggtgcgcc gcccggtgct ccggctggtg
    10501 tggcgggcgc gggtttgaga tcgaccgggc gaccgtctat gaagacgtgg gtgatgcgcc
    10561 ggttcttatc gaagaggtcg ccgcgcacga tggtcaggtt cgcgatcttg cccgtttcaa
    10621 tcgtgccgag ttgagcttcg acgccgagaa tctccgccgc ccgcagcgtc aaggcgcgca
    10681 cggcctcgtc gcgcgcgagt ccgttctcga cagacttcga gacgtttgcg agatagtcgg
    10741 acatgttcgc cagacctccc gactggaagg cgaagcgcac gcccgccgcc gcgagtcgcc
    10801 cggcggtctt cggcgcgtcc acgcgttcgc gcaggacgcg aatcggatcg gggtcggctt
    10861 cgggcgactc ggcggtcgtg cggcgcgggt agttgagcga cagcaggacg gggactttcg
    10921 ccgccgccag ccgctccgat acgcgccaac tctccgcgcc gcccgcgatg atcaggcgca
    10981 ggttgaactc ctgcgcgagg tcgagcgcgc gttcgatctc gcgctggcgg tcggcgtaca
    11041 tgacgacggg catctggcgc gcgagcacgg gcaggagcgc gtcgagcgac ttgtcctgat
    11101 ccggacgacg cacgccgcgc gggctttttt cgtagatggc attggcttcg cggtaacgcg
    11161 ctgcgtcgag catcatctgg cgaatggctg cgaagacgcc gaggagcgag ccgggatagg
    11221 tgcccgtgcg cagcggcgtg aagccgacgt ggagcgcgac gggcgcgcgc acgatcatct
    11281 gttgcggcgt ctcgcccgag agattcacga aggccgactg ccccatgaag atgccttcgc
    11341 gcggcgcggt cagggcggcg gtcagcccgg cgttgcgcgc ggcatcgagt tgttcgccgc
    11401 cgggacgcac gatgtcggac gcgagaatct ccggttgcaa cccgacgggc tgcgaagagt
    11461 ttggcgacac gacggcgggc gtcggttgtt gctgcgcggc gagaaacgcg gctacgcctc
    11521 ctcccccgcc cgcgggtcgc gtgggcgcgg cgggcatgcc gagattcgtg ttggcgtcga
    11581 tgaggccggg atagatcgtg agtcccgcgc cgtcgatgat gcgcgcgtcg gcaggcgcgg
    11641 cgacgttcgc gccgacggct gcgatgaggc cgttgcggat gacgaccgtg ccgcgcgcta
    11701 tctccgcgcc ggagacgggc acgatgcgcg cgttcgtgat ggcataagtg tcaatcgcgc
    11761 tgcgctgtgt gaagaagagt agagcggcgg cgcgaggcgg cgcggcgagc atgacggcga
    11821 gcagcgcgac taccagcgag agagagatga cgggcgcgag gcggcggcga ccgtccgtgc
    11881 gtgcagggtc ttttcgcatg atcattttct gccttcgggt ttgcgagtta acgggtgcaa
    11941 gttaaagtgc gcgcagacgc tccccttgcg gagatggcgt gcgcacgcgt gcgctcaaat
    12001 ttttaaagac gaaaattgaa ggggacgcgt ccggcaagag acgccttctc ccgcgagagg
    12061 ccggagaaga gcgaaatttc tcggttgact gccggaggct cgtcctgtta cggaagcgcg
    12121 cgcgagaaag tttcgaagga cttcccgcac gcctgttaag agcaaaactc gaataaacga
    12181 aaataaacga atccgcgccg ggagcagata aaaggcggcg cgtgcgatca atatcgcacg
    12241 cgccgcccgg ttgcgaatgc gtccgtgttg taagacggat gcggcgcaag tgtctagtgg
    12301 ttggcgttag cgttgtgcgc cgcgcctccg gccttggtgt tggcgttcga cgcgttggca
    12361 ttggcgttcg acgcgttagc attcgcattc gccgcgttgg catttgctgc gttggcgttg
    12421 ttggcgttgt tagggacgac gatggccgcg ttgccgttgc gcgcgcctgc gttggtgttg
    12481 gcgttgtcgg tggcggtgca tccggcagag agtgcggcca gtgcgaccag agttgcaagt
    12541 ttcttcatag tctatgtgct cctgtgaaag tgtcttctac ttgcccggac aattccctgt
    12601 cggtcaaatt tgctgccttc gctggcgagc cgggcgcgat ggccgcgacg gtgggaaagc
    12661 cgtgcgggaa gtgcgggcag ctttcattct atgacgttca ggggaagtgt tatttagcgc
    12721 acgcatattt accattgcac cgcaacgaaa gcaagagagc gcaacgttga aggaggaacg
    12781 ttgaagaagg aaggagaaga caggacacgg aatacaggag tcagaataga aggtgcgcgt
    12841 ctcttgcttc tgactcctgt cttctgtttt ctgactcctg cttttcattc ctccttcaac
    12901 gttaccattt gaccgagcct tcctgcgtcg tatcgatctg cgcggtgggc agagattcgt
    12961 tgaagagata gcccttcatg ttatcgaaga gaaagttttg ggcgtcgggg ctggtgaggt
    13021 cgaggccgta gtggttgatg atctggctct gttt
//
LOCUS       DASWNS010000019         7724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_26469, whole genome shotgun sequence.
ACCESSION   DASWNS010000019 DASWNS010000000
VERSION     DASWNS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26469"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1114
                     /locus_tag="VGB76_00460"
     CDS             239..1114
                     /locus_tag="VGB76_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0097395.1"
                     /translation="MRDESLTDVLRDLVTKSSLPLAGAETVFAADSTGFGIPLRRVWF
                     NRHTERREKRRNYIKLHVMVGVESNIITCAEPTEGSASDRTYLGRLVEGTARYFDISE
                     VSADAGYLSGENMRAVLLAGGIPYIAFYKNCVLDAEYKSNFWKDLLYLYKTRHPRFMD
                     RYYLRNNVEASFASMKEKFGGRLRSKSTTGQFNEVLCKALCHNICVLIHSMYQTGIDP
                     TAWAGVQVRPEAAAGLTGAGLKHREKDFAEIKSAAAGRESYVMEKANEDVQQTRKAKR
                     NTARKLAHPTQASLF"
     gene            1643..3994
                     /locus_tag="VGB76_00465"
     CDS             1643..3994
                     /locus_tag="VGB76_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEY0097396.1"
                     /translation="MSGKENQQSDLGLWEINLINEKLLPYLRRLGYKYIEGNVSVPLG
                     SRTLEADCVVYLDEKKEKPFIVVETKSALRQEVTLLEPAVQQAFAVATALGGDVRYIL
                     VTDGSKYQWFERGVGTQSLVPAKYPPRGPNESYQQTLLELPLTPVAGVEQYLSLLESV
                     VDVLRKEGFVFGLRMGVELNRILIAKLHDERVVAGGGGRRFSAEDNNAGVTATKVKQL
                     YEEAMSILGGGGEYQNWSLSAGTLLTVVRLLEPYDISSVTHNVRDRSFWRVFSRLLGK
                     DEGIHTTPAPLAELLTHLADPAEGERIVDPACGTGLLLLEAYGYINTRASGESENDGQ
                     RDPRDTGLMGIELNAEVAELAATNFALNGLPPGHIINADSLLRPELEQSGVREEGYDL
                     VVLHPPLGHVPKNGSFLRDFSVAHNSPRVTLEILFIELSLRLLRRGGRLAAFVPDTFL
                     SSPSLVDAREWLLGDTTPKAIISLPPEAFMPAGHSGKASVLLLEKRRPYDASEPVLVA
                     DVRAVGYDRFGEATRENDLPRLLEVVRQFKDEGTVESSNSTLRTWTVEISALKAKRLD
                     ITSLDPEGHEVVRAVSRGQYPAVKLGEVVEVISGRNFKTYVDPGDDSAILIQAGAVKD
                     LEIDFAETAPHISLRDYETAKRAQLLVGDVLVTSTGQYLGRAAVVEWLAGPAMASGAV
                     TVLRTRGDVLPFFLAAVINSVIGKEQINRLQAAATAQPYIRRADLNEVIIPLPPLAEQ
                     EAVAAQILERLTLAKDLRRQAYALEENAKALIETKFLGGGVNE"
     gene            3987..5066
                     /locus_tag="VGB76_00470"
     CDS             3987..5066
                     /locus_tag="VGB76_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097397.1"
                     /translation="MNDRFSPDSKKVRRLLENLINTLSEETRFLVDAYESVTGSRGKT
                     VSAVNSELATAAMGQETPWVLYDTISTLIRRLPLPSRAPIIEAVEIKTGHANEMLRPN
                     PKGNWQTVWQVDRGGDPEDRYVALDWYAPPDNVRAPIVPTIIIDYIAGCVSLLESNLT
                     LPSLALLLIALESTLWDDLEHKGVSRSRERVTYPPVEWEYKRVQNRLVCTIAGADKDL
                     SGLDAVAKVRPAVGKFEIRKIQVEGTKVILRAEVDDMIAGFFASEVEASRETFTDKGL
                     SEAVQRARKEGSLVTMPAQLDETLVKLRNNLVHLPSGGSLDPPVPVPFGGEFKTFDEL
                     RQDLKFIRDLMHIVVDLINNLYAAH"
     gene            5125..6945
                     /locus_tag="VGB76_00475"
     CDS             5125..6945
                     /locus_tag="VGB76_00475"
                     /inference="COORDINATES: protein motif:HMM:NF022062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HEY0097398.1"
                     /translation="MLRKDDCAAVIKTFRARHGLDASDENIIATVHLMNTHGLDVHAA
                     LDQSSRSGNDRGVDAWHYDEIGRELSIYQSKLTESRAGALRGFGDLEGAAEWIGGIVV
                     GGTVDVVPSDNHCLFGLYTRLSQVRSSLRTIRFALLSLFEKMEVEDSSEYQQFEQSLV
                     GSELNACVRGRPGGRLVVDAESYNLKGGVPAAVKMYAVDKIPEARIGMRRNAHLDLAY
                     VSLHSLVELYRQRGDVLFDKNVRLSLAGTKEARERLVNPMENTLDMITSGRLSPSIFP
                     FYHIGVTIAATSVADEDTNLLNLAAPSIINGCQTITIASEYLKRLEWQADAGALELFK
                     QIKVVAKVVVGTTNEELKEITNSNNRQNPIENWQLFSNEPLHVEIEAALKDIGVFYER
                     QKGKFDSVMKSADNARHYQATRGTYVKVVDLGQIVALSRKNLQWAAKPSEIFINKENH
                     DRIFDRSVPRYPRDIILTANLFKAVRRGLNNYLELPTHANGHTPLIFKKPIVRAHVYY
                     LALLYFYQKDSKRWVRADFSRSLNKIASPRLVEESKSFYQKVVTRVKNWYLDASKNLS
                     VEISKKSMDRFFIDVTTEIGIDDEGGPVPFTATSIDGVEF"
     gene            complement(7215..>7724)
                     /locus_tag="VGB76_00480"
     CDS             complement(7215..>7724)
                     /locus_tag="VGB76_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097399.1"
                     /translation="ALVNGNRANENVRLKAQAVLERRTPKMLFKLEYLEGRQQKKAKE
                     LHLKRNLLANEIHKWAAKFGIDESLWYLWDKDGVVLTSVGAMLPISAAVEENDERQIE
                     LSQAIHVLERGAEQSLPIFNLDYSLMSFLANSALYALRLYVLLPPDKEGVAGEIQISV
                     KQFWDSLED"
BASE COUNT         1813 a         2092 c         2241 g         1578 t
ORIGIN      
        1 ccagaactgt gggcgacaaa gtgacggcgg aagtgattcg taaatacatc caacgtcaca
       61 aagaaaccga agatacgcaa atagacctgt tcgactaaat cactctaaag ccccaccgct
      121 tgcggtgggg tacttttact cgaatctttc gtctcaacgc ttccagtacg acctgaagga
      181 ggcgcaagcc aaggacatga ttagcgcggc accgagcccg aacacgcttt cggaatatat
      241 gcgagatgag tcgctgacag acgtgctgcg agacctcgtc acgaagagca gtctcccgct
      301 cgccggggcg gagactgtct tcgccgcgga ctcgacgggc tttggcatcc cgctccgccg
      361 cgtctggttt aatcgtcaca ccgagcggcg agaaaaaaga cgaaattaca taaagctcca
      421 cgtgatggtt ggcgtagaga gcaacatcat cacctgcgcg gagccgaccg aagggtctgc
      481 cagtgacagg acgtatctcg gacgactggt cgaggggacg gctcgctact tcgacatctc
      541 tgaggtgtcg gccgatgccg gttatcttag cggtgaaaat atgcgggcag tcctgctggc
      601 gggggggatt ccgtacatcg cgttttacaa gaactgcgtg ctcgacgctg agtacaagag
      661 caacttctgg aaggaccttc tctacctcta taagacgaga cacccgcggt tcatggacag
      721 gtactacctg cggaacaacg ttgaggcctc gtttgcctcg atgaaggaga agttcggcgg
      781 ccgcctgcgc agcaaatcga cgacgggcca gttcaacgaa gttctctgca aggcgctgtg
      841 tcacaatatt tgtgtcctca tccactcaat gtatcagacg ggcattgacc cgactgcgtg
      901 ggcaggggtc caagtcaggc cggaggctgc cgcaggtttg acaggcgcag ggttgaagca
      961 tcgcgagaaa gactttgcgg agatcaaaag cgcggcagcc gggagagaaa gctatgtcat
     1021 ggagaaagct aacgaagacg ttcagcaaac taggaaggcc aaacgaaaca cggcccgcaa
     1081 actcgctcat ccgactcagg cttcgctctt ctagggctta aggtaggctg tgaagctctg
     1141 aactctgtgt aactccggta ccgatcaaca gcgtagggtg caggctctta tagtcagcac
     1201 cctgcgttgt taatattcaa cccgattctc ctgtccttgc ctctccttag gggttgcgga
     1261 cgtagcgcga tggcgatgag gagatacatc catgattggt ataggaacga ccgtttcacg
     1321 gtaaggcagc ccatatgtgc gtggcaaagt tagcaactct tcctcacgtt aaacgattcg
     1381 ctggcacgcc gattagataa ttgtgatcag acaccaagag tgctgtttac aaacatgtct
     1441 tagctagcgc aaaatgcgcc tagtcatccg cgcggagtca ctggggccgc gccggcgacc
     1501 cgatatcgac gtcgatagtt cctcataatt caacataccg actacaggta gtcccgacga
     1561 gtttcgccgc agccggtcct atccttgagg actcatcgtc tactctgaag agatattgaa
     1621 ggccaaattt gaaggaggcc caatgagcgg taaggaaaat cagcagtcag acctcggctt
     1681 gtgggagatc aaccttatta acgaaaagct actcccgtac ctccgaaggc ttggctacaa
     1741 gtatatcgag ggcaacgtga gcgtgccgct ggggagtcgg acgctggagg cggactgcgt
     1801 cgtgtacctc gacgagaaaa aggagaaacc gttcatagtt gtagagacta agtccgcact
     1861 ccgccaagag gtgacgctgc tggagccggc cgtccagcaa gccttcgcgg tcgccaccgc
     1921 cctgggcggg gacgtccgat acatcctcgt caccgacggc agtaaatacc agtggttcga
     1981 gagaggtgtc ggcacacagt ccctggtgcc ggcgaaatac ccgccgaggg gaccgaacga
     2041 gtcataccag cagacacttt tagaactgcc cctgacgcct gtggctggcg tcgagcaata
     2101 tctctcactc ctcgaatcgg tcgtcgacgt cctcaggaag gaggggttcg tgttcgggct
     2161 gaggatgggg gtcgagttga accgcatcct catagccaag ctgcacgacg aaagggtggt
     2221 cgccggcggc ggtggccgac ggtttagtgc cgaagacaat aacgcagggg tgacggccac
     2281 aaaagtcaaa cagttgtacg aagaggcaat gtcgatcctc ggtggcgggg gggagtatca
     2341 gaattggtcg ctgtccgcgg gcacactctt aacagtggtc aggctgctgg aaccctatga
     2401 catcagctcc gtcacccata acgtccgtga ccgctctttc tggcgcgtgt tctcccgcct
     2461 tcttggtaaa gacgagggca tccacacaac gcccgcaccg ctcgccgagt tgctgacgca
     2521 tctggctgac ccggccgaag gcgagcgaat cgttgatccc gcgtgcggga cgggactcct
     2581 cctgctggag gcttacggtt atataaacac cagagcgagc ggtgaaagtg agaacgatgg
     2641 acagcgtgac cctcgggaca ctggcctgat gggaattgag ttgaacgcgg aggtggcgga
     2701 gctggcagca acgaacttcg cgctaaatgg cctcccgccc ggacacatca ttaacgcgga
     2761 ctctttgctg aggccggaac tcgagcagtc tggtgtacgt gaagagggat atgatctggt
     2821 cgtgctgcat ccccccttgg gacatgtgcc caaaaacggg tcgttcctgc gagacttcag
     2881 tgtcgcccat aatagcccga gagtcaccct cgagatcctt ttcatagaac tctcgctgcg
     2941 gcttctacgc cgaggcggga gattggcagc attcgtcccg gacacattcc tatcttcgcc
     3001 gtcgctggtt gacgcccgtg agtggttact gggagataca acacctaaag caatcatcag
     3061 cctcccgcca gaagcattca tgccggcggg acattccggc aaggcgtccg tgctgcttct
     3121 cgaaaagcgg cggccttatg acgccagcga accggtactc gtggccgacg tccgggcggt
     3181 gggatatgac aggtttggag aggcgaccag ggagaacgac ctgccgcggt tactcgaagt
     3241 ggtaaggcag ttcaaggacg aaggcactgt cgagtcttcc aacagcacgc tccgcacgtg
     3301 gacggttgaa atttccgccc tgaaggcgaa acggctcgac atcacgagcc tcgacccaga
     3361 aggccatgag gtcgtacgcg ccgtctcgcg cggccagtac ccggcggtta agctgggaga
     3421 ggtcgtggag gtgatcagcg gccgcaattt taaaacttac gtcgatccgg gagatgacag
     3481 cgcgatcctc attcaggccg gggcggtaaa agatctggag atcgacttcg cagaaaccgc
     3541 tccgcacatc tccctgcggg actacgagac ggccaagcga gcgcagctgt tggtaggcga
     3601 cgttcttgtc acttctaccg gtcaatatct ggggcgtgcg gccgtagtag agtggttagc
     3661 gggtcctgcg atggcaagcg gggcagtcac tgtcctgcgg acgcgtgggg atgtcctccc
     3721 gttttttcta gcggccgtaa taaatagcgt tatcggcaaa gagcaaataa acaggctgca
     3781 ggctgcggct actgcacagc catacatcag gagagctgac ttaaacgagg tgatcatccc
     3841 gctgccgcct ctggctgagc aagaggcagt ggctgctcaa atactggaga gattgaccct
     3901 cgctaaagac ctcaggcggc aggcgtacgc gctggaagaa aatgccaaag cgctcataga
     3961 gactaagttc ctggggggcg gggtcaatga atgatcgttt ctctccggat tcaaaaaagg
     4021 tcagacgcct cctagagaac ttaattaaca ctctctcgga agaaactcgg ttcttggtcg
     4081 acgcgtatga atcggtcacg ggtagcaggg gtaaaactgt aagtgccgtt aacagcgaac
     4141 tggcgaccgc agcgatgggg caggagacgc cgtgggtgct atacgacacg atttctacgc
     4201 tgatcaggcg actgcctttg ccgagcaggg caccaatcat tgaagccgtc gagatcaaaa
     4261 ccgggcacgc aaacgagatg ttacgcccga acccgaaagg caactggcag accgtttggc
     4321 aagtcgatag gggcggggac cccgaggacc ggtacgtcgc gttggactgg tacgcgccac
     4381 cggacaacgt cagggcaccg attgtcccaa caattatcat tgattacatc gcgggctgcg
     4441 tttctctgct ggagagtaat ctcaccttac cttcgttagc actcctgctc atcgccttgg
     4501 agtccacact ctgggatgac ctcgaacaca agggtgtgtc tcggagccgt gaacgagtga
     4561 cttacccgcc tgtggagtgg gagtacaagc gcgtgcagaa taggctagtc tgcacgatcg
     4621 ccggtgctga caaggaccta tctgggttgg atgcggtggc gaaggtaagg ccggcggttg
     4681 gcaaatttga aatccggaag attcaggttg aaggaacaaa ggtaatcctg agggcagagg
     4741 ttgatgacat gatcgccggg ttctttgctt ctgaggtgga ggcgagccgt gagacattca
     4801 ccgacaaggg gctctccgaa gcggttcaga gggcaaggaa agagggttcc cttgtgacga
     4861 tgccagcgca actcgacgag actcttgtca agttgcggaa taacttagtt cacctaccgt
     4921 ccggcggcag cttggacccg cccgtaccag tcccttttgg gggagagttt aagacctttg
     4981 acgagctgag gcaggatctc aagttcattc gagatctgat gcacatagtc gtcgatctga
     5041 ttaataacct atacgccgct cattaaacgg aacccgggga gcgtttcaac gagtagcctt
     5101 cacaaacatt gccaggacga aacaatgctg agaaaagacg actgcgccgc agtcattaag
     5161 accttccggg cgaggcacgg gctcgacgct agtgacgaga atatcatcgc caccgttcac
     5221 ttaatgaaca cacacggact cgacgtgcac gcggccctcg accagagttc gaggagtggc
     5281 aatgatcgag gcgttgatgc atggcattac gacgagatcg gccgtgaact ctccatctat
     5341 cagagtaaat tgaccgagag cagagcgggc gccttgcgcg gattcgggga tcttgagggt
     5401 gccgccgagt ggataggcgg gatagttgtc ggtgggaccg tcgatgtcgt gccgagcgat
     5461 aaccattgcc tcttcggcct gtacactcga ctatcacagg tccgcagttc cctcagaacc
     5521 atccgcttcg cactcctatc gctgtttgag aagatggagg tagaggattc ctccgagtat
     5581 cagcagttcg agcaatcgct agtcggctct gaactgaacg cgtgtgtcag agggcgtccc
     5641 ggcggtaggc ttgtcgtcga cgccgaatct tataacctga agggcggagt ccctgcggct
     5701 gtgaagatgt acgccgtaga caagatcccc gaggcgcgga tcgggatgag acgcaacgcc
     5761 cacctcgatc tggcgtatgt ttccctccac agcctagtgg agctataccg gcagcgtggg
     5821 gacgtgctct tcgataagaa cgtccgcctc tcgctggctg gcactaagga ggcgagggag
     5881 agactggtga acccgatgga gaatacgcta gacatgatca catcggggag actgagcccg
     5941 agcatcttcc ccttctatca cattggggtg accatcgctg ctacttccgt tgccgacgaa
     6001 gacaccaacc tgctgaacct cgcggcacca agcatcatca acggctgcca gaccatcacc
     6061 atcgcgagcg agtatctaaa gcggctcgag tggcaggcag acgctggtgc cttggagctg
     6121 ttcaaacaga ttaaggtcgt cgctaaggtg gtcgtgggaa caacgaacga ggagctgaaa
     6181 gagatcacca attccaataa ccggcagaac ccgatcgaga actggcaact cttctccaac
     6241 gagcccctcc acgtggagat cgaggcagcg ctaaaagaca ttggagtctt ctacgagagg
     6301 cagaagggta agttcgactc ggtgatgaag agcgccgaca atgcgagaca ctatcaggcc
     6361 accagaggaa cctatgtcaa ggtagttgac cttgggcaga tcgtcgccct ctccagaaaa
     6421 aacttacaat gggcggcgaa gccgtcggag atcttcatca acaaggagaa ccacgaccgc
     6481 atttttgacc gctctgttcc ccgttacccg cgcgacatca tccttacagc gaacctgttt
     6541 aaggccgtca ggcgggggct gaataactac cttgagctgc cgacgcacgc caatggtcat
     6601 acgccactca ttttcaagaa gcctatagtt cgggcccacg tctactacct cgcactactc
     6661 tacttctatc agaaggacag caagaggtgg gtgcgcgccg acttctctcg atcgcttaac
     6721 aagatcgcaa gcccccgcct cgtcgaagag tccaagtcgt tctaccagaa ggttgtgaca
     6781 cgggttaaga attggtacct ggatgcctcg aagaatctca gcgtggagat ttccaagaag
     6841 tccatggaca ggtttttcat cgatgtcact accgaaattg ggatagacga tgaaggcggc
     6901 cccgtaccat tcaccgcgac gagcattgac ggcgtcgagt tttagacgtc aatgctcagt
     6961 gtttcgcgga tgactatcag tcatagacgc atcgctccat gacactgctc gggaacgtcg
     7021 gcaccaggtt ccacgctggc gtgcgtatcg cagagcgaat cgggagttct aaacagtctg
     7081 tgtaagtggc aataatcagc acccactcac acagactgtt tagaattgcc tcaactccga
     7141 gtcatacatg gctgcgtgac ggtgcaaccg cgtcgctgac tcatagctat cctgcgctaa
     7201 gggcagggaa ttggttaatc ttccagcgag tcccagaatt gcttaacaga gatttgtatc
     7261 tcccccgcga ccccttcctt atctggcggg aggagcacat ataggcgtaa agcgtagagg
     7321 gcggaattgg ctaggaaact catcaaggaa taatccagat tgaatatcgg cagcgattgt
     7381 tctgcgcctc tctctaggac gtggatggct tggctcagtt cgatctgacg ctcgtcattc
     7441 tcctcgacgg cggccgagat gggaagcatg gctcccacac tggtgagcac tactccgtcc
     7501 ttgtcccaca ggtaccaaag gctttcgtcg attccgaact tggccgccca cttgtgaatc
     7561 tcattcgcca gtaaattcct tttgaggtgt agctccttag ccttcttctg ctggcgaccc
     7621 tccaagtatt cgagcttgaa tagcattttc ggggttctgc gctcgaggac agcctgcgcc
     7681 ttaagcctca cgttctcatt cgcccgattg ccattcacca acgc
//
LOCUS       DASWNS010000020         4953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_416098, whole genome shotgun sequence.
ACCESSION   DASWNS010000020 DASWNS010000000
VERSION     DASWNS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416098"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VGB76_00485"
     CDS             complement(<1..442)
                     /locus_tag="VGB76_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097400.1"
                     /translation="MSTPTDQPSPLAYPYQTEPSPEGTNSPPSGDAPLSGGPASAAAF
                     NPDNPPWGLLAAVVVVLGSLALVVLLQLVGTVPYFIWRAANGLPLMETAQPDKNVILI
                     AILAMLPAHVLTLTLAWLIVTSRGKRPFWSTLGWWWSPRYGWLAD"
     gene            complement(599..1150)
                     /locus_tag="VGB76_00490"
     CDS             complement(599..1150)
                     /locus_tag="VGB76_00490"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0097401.1"
                     /translation="MDTSGKEAPEVVASEDVYRGRIFTITVDTVREGGREHQREVVRH
                     PGSAAIVAVHDDVTVSLVRQYRHPTVKYLLEIPAGSRDNFEPPEECASRELEEELGLV
                     AGEWEKLSEFFVSPGFCGEKMWVYLATGLTETKANPEEDEDLEIVRLPLTRALEMIEA
                     GEIEDAKTIIGLTLAAARLSVNG"
     gene            complement(1233..2579)
                     /locus_tag="VGB76_00495"
     CDS             complement(1233..2579)
                     /locus_tag="VGB76_00495"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY0097402.1"
                     /translation="MPSNPRSEIRVPQFDEAVKYLLSLGHETLAIKLGLANIEQLLAA
                     LLSPEHAYPAIQIAGTNGKGSTAAMLESICRACRVRAGLYTSPHLISITERIRIDGEE
                     ISREEFARLTAQVRRVSLELQEASRALPTFFEQVTAIALLAFREAGVSLAILETGLGG
                     RLDATTAARARTLAITPIALDHQEYLGHTLREIAAEKAAIIRRGAAAVVVAPQTDEAR
                     EVLLARCREQQVAPRFATRDINVRGADEEGRLRVTFKTGSDIYEEVRLSLRGRHQVTN
                     AAVAIALAEVLRDEHNFPVEREQIIEGLETATHAGRLELQAGKPSLLFDGAHNPAGAR
                     ALRDYLDEFVRVPVTLVFGAMRDKELDEIAATLFPAAHRLILTRPANARAATLDTLKR
                     LAASHSVDSSPIFTSSPVEALRVAVESTPPEGIVCITGSLYLVGEVKSLNARREQG"
     gene            complement(2611..3600)
                     /gene="accD"
                     /locus_tag="VGB76_00500"
     CDS             complement(2611..3600)
                     /gene="accD"
                     /locus_tag="VGB76_00500"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY0097403.1"
                     /translation="MTWFRKKGQKIEVVPADERVVKTEGVFRKCPECDAALYKKDLEE
                     SLQVCPPCGHHFRIGARERLAMLFDNAGYEELDAEVISSDPLHFVDTKPYTQRLEQAK
                     RATGLPEAVINARGTIDGHTVFAGAMDFSFLGGSMGSAVGEKVTRLIERAIKERAAVI
                     TITSSGGARMQEGTLSLLQMAKVSAALAQLDKSRLPFISILTDPTTGGVTASFAMLGD
                     VIIAEPKALIGFAGPRVIEQTIRQKLPKDFQRSEFLLEHGMLDAVVDRREMRALIIKL
                     LGFMVNPEIKATHLDVNIDFAGSNGYNSKSARAVLEEQPPEAKIQQPETQSTD"
     gene            complement(3770..4849)
                     /locus_tag="VGB76_00505"
     CDS             complement(3770..4849)
                     /locus_tag="VGB76_00505"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEY0097404.1"
                     /translation="MLVQVVELIEAKRRFAITSHIRPDGDGLGSSLGLYWLLKALGKE
                     PEVIMRDPVPHSYRKLPGAETIRVTPVIDKQYDAVFVIECSDITRPGLADLDKQYVVN
                     IDHHATTSPFGTINWIDSTASAVGEMIYNLCKAIGVRPSKEIAECVYTALVTDTGSFH
                     YSNTTERTFKVASELLRAGVKPAKVSQAVFNNYPWSKLELLSQVLPTARHDETGRVAC
                     LRQTLEMQERAGASDEDSDGFVNYPMSVGDVEACAFLKETAPGIYRTSLRSKGDVNVA
                     RIAEKFGGGGHRNAAGCTLTGEWDEVERKIVALLVEAVDNRSNGNSSSNGSLSDQTPT
                     ANGGGNTPTGQLPATFIRGTLNAER"
BASE COUNT          912 a         1448 c         1595 g          998 t
ORIGIN      
        1 tgtctgcgag ccaaccgtag cgcggactcc accaccagcc cagcgtgctc cagaaagggc
       61 gtttgccccg gctcgtcacg atcagccacg cgagcgtcag cgtcaggacg tgcgcgggca
      121 gcatcgcgag gatcgcgatc aggatcacgt tcttgtcagg ctgcgccgtc tccatgagag
      181 gcagcccgtt cgcagcgcgc cagatgaagt aagggacagt gccgacgagt tggaggagga
      241 cgactagggc gagacttccc agcacgacga cgactgccgc cagcaatccc cagggcgggt
      301 tgtccggatt gaaagccgcc gcgctcgccg gaccgccgct caacggagcg tcgcccgacg
      361 ggggcgagtt cgtaccctcc ggcgaaggct cagtttgata aggatatgcg agaggggaag
      421 gttgatcagt cggtgtgctc acgaaaatga gtgtaagaga gcgggcgcgg cagcgtcaac
      481 cggaaggcga agaagggatg ggagagaggg aaaaagggaa ggagaaaagg ggaaaggttg
      541 aaagcaaccg gcgcacgatg gacgcgcctc gttgctttca acccttcccc ttggccccct
      601 acccgttcac actaagccgc gcggcggcga gcgtgagtcc gatgatggtc ttggcatctt
      661 cgatttcgcc cgcttcgatc atctcaagcg cgcgcgtgag gggcaggcgc acgatctcca
      721 aatcttcatc ctcttcgggg ttggctttgg tttccgtcaa accggtggcg aggtagaccc
      781 acatcttctc cccgcaaaaa ccgggcgaga cgaaaaactc ggagagcttt tcccactctc
      841 ccgccaccag ccccaactct tcttccaact cgcgcgacgc gcactcttcc ggcggctcga
      901 aattgtcccg gctccccgcc ggaatttcca gcaggtactt caccgtcggg tggcgatact
      961 ggcgtacgag cgacacggtc acgtcgtcgt gcacggcgac gatggccgcg ctgccgggat
     1021 ggcgcacgac ctcgcgctgg tgttcacgtc cgccctcgcg caccgtgtca accgtgatgg
     1081 tgaagatgcg accgcgatac acgtcttcgc tcgccacaac ttcgggagct tccttgccgg
     1141 atgtgtccat aaatttttac gagatatgac aggcttaaat ttcgtacgaa cgaatcgatc
     1201 ttagagcaat ctaaaacttt actcgttttg attcaaccct gctcgcggcg cgcgttgaga
     1261 cttttaactt cgccgaccag atagagcgac cccgtgatgc agacgattcc ttccggcggc
     1321 gtgctctcca cggcgacgcg caaggcttcg acgggcgacg aagtgaagat cggcgaactg
     1381 tcaacgctat gcgacgcggc gagtcgtttg agcgtgtcga gcgtggcggc gcgcgcgttt
     1441 gccgggcgcg tcaggatcag tctatgcgct gccggaaaaa gcgtggccgc gatctcgtcc
     1501 aactctttat ctcgcatcgc gccgaagacg agcgtgacgg gaacgcggac aaactcgtca
     1561 aggtagtcgc gcagcgcacg cgcgcccgca gggttgtgcg cgccatcgaa gagaagggaa
     1621 ggcttgcccg cttgcaactc aaggcgtccg gcgtgcgtcg ccgtttcgag tccctcgatg
     1681 atctgttcgc gctcgacggg gaagttgtgc tcgtcacgca aaacttccgc gagcgcgatt
     1741 gccacggcgg cgttcgtcac ctgatgccgt ccgcgcaggg acaagcgaac ttcttcgtaa
     1801 atgtcggagc cggtcttgaa cgtcacgcgg agccgccctt cttcgtctgc gccgcgcacg
     1861 ttgatgtcgc gcgtggcgaa gcgcggcgcg acctgttgct cgcggcaacg cgcgagcaac
     1921 acttcgcgcg cctcgtccgt ttgcggcgca acgacaaccg ccgctgcccc tctgcggatg
     1981 atggcggcct tctcggcggc gatctcccgg agcgtgtggc cgagatactc ttgatggtcg
     2041 agcgcgatgg gtgtgatggc gagcgttcgg gcgcgtgcgg cggtcgtcgc atcgagacga
     2101 ccgccaagac ccgtctccag aatggcgagc gagacgcccg cttcgcggaa ggcgaggagc
     2161 gcgatagccg tgacctgctc gaaaaatgtc gggagcgcgc gcgacgcctc ttgcagttcc
     2221 agcgataccc ggcgcacctg tgccgtgagg cgcgcaaact cttcgcgcga gatctcttcg
     2281 ccgtcgatgc gtatgcgttc ggtgatcgag atgagatgcg gcgaggtgta aagaccggcg
     2341 cgcacacgac acgcgcggca gatgctctca agcatcgctg cggttgagcc tttgccgttc
     2401 gtgcccgcga tctgtatggc ggggtaagcg tgttcgggag acaggagcgc ggcgagcagt
     2461 tgttcgatgt tggcgaggcc gagtttgatg gcgagcgttt cgtgaccgag actgaggaga
     2521 tatttaacgg cctcgtcgaa ttgcggaacg cggatttcgg aacgcggatt agaaggcatg
     2581 actatgtaat ccgcaatccg caattgccgg ttagtctgtg gactgagttt cgggctgctg
     2641 aattttggcc tcgggtggtt gctcttcgag cacggcgcgt gcggacttcg agttgtagcc
     2701 gttgctgccc gcgaagtcta tgttcacgtc aaggtgcgtc gctttgatct cgggattcac
     2761 catgaagccg agcagcttga tgatgagggc gcgcatctcg cggcggtcaa ccacggcgtc
     2821 gagcatcccg tgttcgagca gaaattcgga gcgctggaaa tctttcggca acttctggcg
     2881 gatagtctgt tcgatgacgc gcggcccggc gaaaccgatc agggctttcg gttcggcgat
     2941 gatcacgtcg ccgagcatgg cgaagctggc ggtgacgccg ccggtcgtcg ggtcggtcag
     3001 gatggagatg aaagggagac gcgacttgtc gagttgcgcg agcgcggcgg aaactttcgc
     3061 catctgcaag agcgagagcg tgccctcctg catgcgcgcg ccgcccgaag aggtgatggt
     3121 gataactgcc gcgcgctctt tgatcgcgcg ctcgataaga cgcgtcacct tctcgccaac
     3181 cgccgatccc atcgagccgc cgaggaaact gaaatccatc gcgcccgcaa agaccgtgtg
     3241 cccgtcgatt gttccgcgcg cgttgatgac ggcttcgggc agcccggtgg cgcgtttggc
     3301 ctgttcgagt cgttgcgtgt agggcttggt gtcaacgaag tggagcgggt cggaggagat
     3361 aacttcggcg tcgagttctt cgtagccggc gttgtcgaag agcatggcga gccgttcgcg
     3421 tgcgccgatg cggaagtggt gcccgcaggg cgggcagacc tgcaaagact cttcgaggtc
     3481 tttcttgtaa agcgcggcgt cgcactccgg acacttgcgg aagacacctt cggtcttgac
     3541 gacgcgctcg tccgccggaa caacttctat tttctgtccc tttttacgga accatgtcat
     3601 aggcgcagag tataaacgaa gtgccggagg ctgtcagtag cccgtcgcgc ttctcgcgtt
     3661 gtcagtcgcc gtttgttttt cgtccgaccc cggcctggac tcaatcttta gagagcaaac
     3721 tgcccggtca cagacgacgg acgaccggct aacgtgcacg cagagcgttc tatctttccg
     3781 cgttcagcgt gccccggatg aacgtcgccg gaagttgtcc ggtcggcgtg ttgccgccgc
     3841 cgttcgccgt gggcgtctgg tcggacagcg aaccgttgct gctgctgttg ccgttcgaac
     3901 gattgtccac ggcttccacg agcagtgcga cgatctttcg ttcaacctcg tcccattcgc
     3961 ccgtgagcgt gcaaccggcg gcgttgcgat ggccgccgcc gccgaatttt tccgcgatgc
     4021 gcgccacgtt cacgtcgccc ttcgaacgca ggctcgtgcg atagatgccc ggcgctgtct
     4081 ccttcaaaaa ggcgcacgct tcaacgtcgc cgacgctcat cgggtagttg acgaagccgt
     4141 ccgagtcttc gtccgaagcc cctgcgcgtt cctgcatctc cagcgtttga cgcaggcacg
     4201 ccacccgccc tgtctcgtcg tggcgcgcgg tcgggagcac ctggctcagg agttcgagct
     4261 tgctccaggg gtagttgttg aagacggcct gcgacacctt cgccggtttg acgcccgcac
     4321 gcaataactc ggaggcgact ttgaaggtgc gctcggtggt gttcgagtag tgaaacgatc
     4381 ccgtgtcggt gacgagcgca gtatagacgc attcggcgat ctctttcgag gggcgcacgc
     4441 cgatggcctt gcacaggttg tagatcatct cgccgacggc tgaggcggtc gagtcgatcc
     4501 agttgatcgt gccgaaggga gaagtcgtcg cgtggtggtc tatgttgacg acatattgtt
     4561 tgtcgaggtc tgcgaggccg gggcgcgtga tgtcggaaca ttcgatgacg aagaccgcat
     4621 catactgttt atcgatgacg ggggtgacgc gaatggtttc cgcgccggga agtttgcgat
     4681 aagagtgggg cacggggtcg cgcatgatca cttccggttc tttgccgaga gccttgagca
     4741 gccagtaaag acctagtgac gatcccagac cgtcgccgtc cggacgaatg tgcgaggtga
     4801 tggcaaagcg tcgcttcgcc tcaatcagtt cgacgacttg aacaagcatg gttttcaaaa
     4861 aggatgaggg atgagggatg agggatgaat caggaaaact actcaaccga gtgagtgctt
     4921 cctcttattc atccctcatc cctcatccct cat
//
LOCUS       DASWNS010000021        14556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_311920, whole genome shotgun sequence.
ACCESSION   DASWNS010000021 DASWNS010000000
VERSION     DASWNS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14556
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311920"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1171)
                     /gene="gcvT"
                     /locus_tag="VGB76_00510"
     CDS             complement(38..1171)
                     /gene="gcvT"
                     /locus_tag="VGB76_00510"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY0097405.1"
                     /translation="MTTAPETLKQTPLNAAHRRLGARMVDFGGWDMPVQYPAGTVEEH
                     LRTRRRSGLFDVSHMGEIEVRGAEAIPFVNRLCSNDVTKLVDGQAHYTALTTPEGTVV
                     DDLLVYRFAEDHLLLVVNASTTGKDWDWINSHRGNEAVELTNRSADFCQIAVQGPDAV
                     RILQTLTGTPLEEIKYYHFREGTVDGVPSIISRTGYTGEDGFEVYAAPDKAEQLWDRL
                     LEAGAYGTPEGILPCGLAARNTLRLESAMALYGHEIDETTTLYEANLGWICKLNKGDF
                     TGRERLAQQKAEGVTRKLVGFEVTDRGIARDGYDVLVNGEKLGRVTSGSPAPYLKKNI
                     GLAYLPTQHTDVGQEIQIDVRGRPVAARIVPVPFYKRERAAAK"
     gene            1771..6039
                     /locus_tag="VGB76_00515"
     CDS             1771..6039
                     /locus_tag="VGB76_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEY0097406.1"
                     /translation="MSFPVPQSLPPARRRLRFALFFLLSLSLYASGASLFSHATVEAA
                     PRVQKKKGQAKVRRGNVRRARRGGVAASTSRTPTARPAPVAEPSIGAQEDGDERDVID
                     ARQQWFMMQRAYPFDTPPAGARLKAYLARPKDAGRGKDGSSAYAAVQTWRSIGPSPTT
                     PAFPNNWGLTSGRLNAIAVHPTNPQIILLGASTGGIWRSTDGGANFTPVGDTQVDLSV
                     GSIAFSKSNPSIVYAGMGDLGNGYFGTGVLKSTDTGATWTRSSNNTLPPLGTTAEVEV
                     DPANPNRVYVLQASRTMTTSYDILHPDGALYSSGFYLSEDGGVNWRRTLPGRPRDLAV
                     HPTNSQILYLGMNSVTALGINAGLYKSLDGGATWLPAPVYTAPTGISTSDVRVAVTPA
                     SPENVYVIAGTRTALTVTVSNNGGLTWTTKTSAGVDPGQFGYNSYIYAAPNDPNTIYV
                     GTRDVFKSTNGGSSWTNLTKNFVPNPARTDCSFCYSPFGSNSHPDQHAFAFAPNDPNT
                     LYIGNDGGISKSSDGGVSFVSLNSSLTLTQFVGISMHPTDPNISYGGTQDNGTQLRIP
                     GTNTWREFSAGDGGRSIVNAPNPSIVFTTYIYGRVNRFTNNGQTFSGTIAENTTFGEP
                     TSGHRIAFYPPMTGNGVDSTIYFGTYRLFTCTNCHDTSRSHPNAPANWSAPGDTFDQT
                     KGNGDVLSAIGVERRANAQVIYTGSAQGRIMVTENGGQTWIDISAGMPNRFIEHITVD
                     PSNPVVAYVTYGGFGTGHVFRTTNFGTSWTDIGGIPGQPNTIPNVPVSTFLIDPTTPS
                     TLYAGTDIGVFRSTDHGATWATFNKGMLPAVVTGFATGANGAIQLSTYGRGAYELVTT
                     TPPTYSIGGLVTLGGVGLSGVTLSLSNGQAALTTTTDASGNYSFSGVTGGDNYVLTPS
                     KPNFNFAPQNMTYSNLGAHQSANFNAASACLYSVAPQGLPRFAASGGAGSFQITTSAG
                     CAWTASSLVPWITITNGSGTGNGTVSFNVAPNTGTARNGQILHTNDSFYNITQEASPS
                     PALVAFGAASYTFGEEAGRATITVTRTGNTSGAVSVEVATVDDPAAIPCNPTTRDQNG
                     NLFPQGKAFARCDYATSIETVTFAAGDTQPKSVFVPLVDDGHVEGAETFQITLLNASN
                     AALGTLSTTTLTLTDNDAPGQANPILQTPLFVRMQYLDFLSREPEPGEPWSGVLNRCP
                     LPFNLDPLNASAGCDRIIVSKSFYESQEFRLKGLYTFIFYRVAFNRLPEYAEIIPDMR
                     SVTGATAAEVYARRALFPSNFAQRQEFRSAYDALSHTAYVNALLDRYGFASITSPDPQ
                     NPEGGTKVTLSRADLIARLGAQGASNLTRAQVLRAIVESDEMRAAEFTRAYVAMQYYG
                     YLRRTPEPGGFNAWLNYLNAHPTDARTMVNGFVNSGEYRARFGQ"
     gene            complement(6132..6887)
                     /locus_tag="VGB76_00520"
     CDS             complement(6132..6887)
                     /locus_tag="VGB76_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0097407.1"
                     /translation="MTCEETQKSFSPYLDGVLARASRAQVDAHLEVCPVCRLELAHTR
                     SITRALAGLERPRVPHNLAGSINELLLIERAARRQQPTGSLPTRFMRWLEPRLMPYTV
                     GALASMILFVGVVNALRPQFRVLRELAIATRQASEESLDTSLFEGEGYDVTKPVPTIN
                     PNGALAALIRTPSSSDDDDADDMVIYADVFSNGSASLAGVVQAPRNPQMLDEVQEALR
                     RTPAFVPAYRDGRPQTMRVVFSVSKVNVHDRSF"
     gene            complement(7375..8037)
                     /locus_tag="VGB76_00525"
     CDS             complement(7375..8037)
                     /locus_tag="VGB76_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0097408.1"
                     /translation="MSFGEPIALRGQAELEHPAVAAESAESRPSPELQFIERLRAGEA
                     AAFDHLVNERSADIYALLYRLTEDPEEARDLTQETFLQAFRSIAHFRGDADLRTWLYR
                     IAVNQARNRWRWWRRRRRNVTVSLDATDETHELSLATRLADERADDPEERTLAHEREE
                     ILLAAVRTLARPYREAVVLRDVEGLSYEEVAATLEISVGTVKSRLSRGRLELRRKLEG
                     SL"
     gene            8356..9171
                     /locus_tag="VGB76_00530"
     CDS             8356..9171
                     /locus_tag="VGB76_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097409.1"
                     /translation="MAPSRKKHAREVQRDKFREKTLAQFDRVGDRLEGKGRTLLYALA
                     GLVGLALLAGVYSWWSGRKAEEATRALGRVTEIVNATVTPSPMPGSTGLSFTSEKERA
                     QRVVEEAQKVEQKYGDPYKSLAHYFRAANLLTVDRSKGLSELEALTKNGDAEVSARAK
                     FALAQAREADGQHDAAAALYLELVRAQNPAVPVDTANLRLASIYEKQGKKSEAADILF
                     QIAKTAREAKDKDGKPSTQSAAAREAAQKLERLDPARHAQLPAEPAPATNLPF"
     gene            9261..10406
                     /locus_tag="VGB76_00535"
     CDS             9261..10406
                     /locus_tag="VGB76_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY0097410.1"
                     /translation="MDNLTHTLVGLTAAKAGLERLSPGATAVCMLAANAPDADIVATF
                     GGSWFYLKHHRGITHSIVGTLTIALLLPLLFYAGDRLIARLRARPPRVRLRGLVIASL
                     LLSASHPLMDWTNNYGLRPLLPWSGRWFYGDLVYIIDPWLWLSLGGASFLLTAKTRWR
                     SVAWGALALLATVAFVVLPLRVGETYPHTSRALWLVWLACLIVAHRMKVAPRLGSSIA
                     LAALAFVVLYWGALGFVHGHAVERARAVAEAVAARGGESVLRVAAMPSLADPSSWRVL
                     AETDRATYRFNISLLARVETEAAAPAALARFEKPQGEAAELAARAAVDERAEIFLDFA
                     RFPAVRVETQNCAEELLVQFADLRFTEPGSGRRAGNFSLEVPVRPAR"
     gene            10575..12710
                     /locus_tag="VGB76_00540"
     CDS             10575..12710
                     /locus_tag="VGB76_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0097411.1"
                     /translation="MKFASHEVGNSSLTARESPGEASDPPVTEASDGRFLTFAESAAD
                     AIIVIDAASDIRFINRAAEKIFGYPVHELLGRNLTILMPGYLRHVHREGLAQYLSTGV
                     PHINWSSVELPGLHRDGREVALEISFGAFSENGVRYFTGIARDITERKRQKEAQRFLV
                     EASALLAASLDYETTLQSVAHLSVPFLADWCIVYVVDDDAAIRPIAVMNDDAAQVELV
                     WELERRENQNPEALTGVPKAIRTGQSELYADIPTAAETSAASDAGRFRVARQLDLKSA
                     MIVPLVARGRTLGAITFATAVSGRRYAPSDLAFAEDLAQRAAFAVDNARLYREAQEAN
                     RLKDEFLATLSHELRTPLTSILGWTNLLRSVKFDESTTERALETIERNARSQKQLIDD
                     LLDASRIITGKLRLELKPTELKSVVESACHAARPAADAKGIELSLALEGASSIVAGDA
                     ERLQQVVWNLLSNAVKFTPEGGRVEVRLERVGTQAEISVSDTGTGIKAEFLPFVFERF
                     RQADGSTTRTHGGLGLGLAIVRHLVELHGGTVVAESTSAGATFKVSLPLIEYAKRGET
                     ATAGELRFEGVGEPTGRQALLRGLHILIVEDEPDTRVLLKKALERYGAQVETRELAEE
                     ALDALAASAFDVVLSDIAMPGEDGYSFIEKIRARGYTTPAIALTAYAREEDRQLALAS
                     GFQLHLAKPVAPKELAAAVADVVGRTSNV"
     gene            complement(12741..13844)
                     /locus_tag="VGB76_00545"
     CDS             complement(12741..13844)
                     /locus_tag="VGB76_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0097412.1"
                     /translation="MSTGETQPRVMSSAYMHWAKTQSQATYNLATSGVRPYTLEQLRV
                     ELSDLELSGVSTYGYQPLQQAIAAKCRVKADSVVAANGTSMANFIALATLIEPGDEVL
                     IEQPTYELLLSAALYLGAKVTRFTRHTEARFRIDPDEVARAVTPRTKLIVVTNMHNPS
                     NAYEHDETISQLGEIAHSVGARVLVDEVYLDAAFSDAPQSAFHLGELFITTSSLTKVY
                     GLSGLRCGWILAAPALAARMWRLNDLFGAVQPHASERLACLAFARLPEIAAHSRALLE
                     RNRLLANDFLETRRDDLDAFPITHGMIAFPRLRRGSVEDLCALLREKYETSIVPGRFF
                     EMPEHFRLSIGGDTGMLSGGLERLSAALDEIKR"
     gene            complement(13844..>14556)
                     /locus_tag="VGB76_00550"
     CDS             complement(13844..>14556)
                     /locus_tag="VGB76_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0097413.1"
                     /translation="MFAYGGWQTTSFIAGEMRDPRRDLARGLLFGVAGVILLYVAVSF
                     VCVYALGAEGLAQTNTPATAVMRRALGERGATLIALGISISTLGFLSQSLLTTPRVYF
                     AMAEDGLFFHRIAQVHTRTRVPVFAIALQGALAIIVALSGKYGQILNYVVSVDFIWFG
                     LTGLSVFIFRRRDPAGEATFKVPGHPYTTALFVAACWLVVLSTVYKYPVNSLIGFAIV
                     LAGVPACYYWLRQQRRDR"
BASE COUNT         2784 a         4640 c         4424 g         2708 t
ORIGIN      
        1 acaccgcact catcacttct atttcgccgc tgcgcgttta tttcgccgct gcgcgttcgc
       61 gtttataaaa agggacgggg acgatgcgag cggcgaccgg gcgaccgcgc acgtcaatct
      121 gtatctcctg tcccacgtct gtgtgctgcg tgggcaggta ggccagcccg atgttcttct
      181 tcaaataggg cgcggggctg cccgaagtga cgcgccctaa cttctctccg ttcaccagca
      241 cgtcgtagcc gtcgcgtgcg atgccgcgat ccgtcacttc gaagccgacg agtttgcgcg
      301 tcacgccttc ggccttctgc tgtgcgagac gttcgcgccc ggtaaagtcg cctttgttca
      361 atttacaaat ccaaccgagg ttggcttcgt agagcgtcgt cgtctcgtcg atctcgtggc
      421 cgtagagagc cattgcggat tcgagccgga gcgtgttgcg cgcggcaagc ccgcagggca
      481 ggatgccttc gggcgtgccg taggcaccgg cttcgagcag gcgatcccag agttgttcgg
      541 ccttgtcggg cgcggcgtag acttcaaagc cgtcttcgcc cgtgtagcct gtgcgcgaga
      601 tgatcgaagg cacgccgtca accgtgccct cgcggaagtg gtaatacttg atctcttcga
      661 gcggcgtccc ggtgagagtt tgcaggatac gcacggcgtc gggtccctgc acggcgatct
      721 ggcagaagtc cgcgctgcgg ttggtgagtt ccactgcctc gttgccgcga tgcgagttga
      781 tccaatccca gtccttcccg gtcgtcgagg cgttgacgac gagcagcagg tggtcttccg
      841 cgaagcgata gacgagcaga tcgtcaacga ccgtgccttc gggcgtggtc agagcggtat
      901 agtgcgcctg tccatccacg agcttggtga catcgttcga gcagaggcga ttgacgaagg
      961 ggatggcttc cgcgccgcgc acctcgatct cgcccatgtg cgacacgtcg aaaagaccgg
     1021 agcggcggcg cgtgcgcagg tgttcttcga ccgtcccggc ggggtactgc acgggcatgt
     1081 cccagccgcc gaaatcaacc atccgcgcgc cgaggcgtcg atgcgcggcg ttgagcggcg
     1141 tctgcttaag tgtctcgggc gcagtagtca agcggctctc ctgactctct gaatgagatg
     1201 tgtagaacgc tgtaaattat tgatgttgac gaagaagaaa acctaacatg ccgcgcgcaa
     1261 gtaggtcaag caagtcggcc tgtgaccggg aggcgcggtg cgggacgcat cttatggaag
     1321 cggaagcgaa agagaaggcc agaaacgctg ctctccgaac gtcgccgggt tcgccataaa
     1381 acttgacgga gcgtgaaatt cgatagtaat gttggccgag cctcgcggtt cgaggaacca
     1441 gccggaaaat ccctttttag tcaatgtcgg ttgacccgtc gggcatcctg aaactcgttt
     1501 tttgagtcta aaatcagacc tttgggaact cttttcaagc ggtttcgccg gttttcgttc
     1561 aactcagcga caattcagcc ttaactttac tggccgcaga cacccccttc atgccagtcg
     1621 caaacttttc ctataacttt tacacaggga gtcgcccgtt cgtccctgct ccggcttcgc
     1681 attcatgcgg cctttccggc tcatcccacg cgtaccaaac gcagcgtcca ttcttcgtcc
     1741 tgtgattgtg aggattttgc tttgaagaaa atgagtttcc ccgttccgca atctctccca
     1801 cctgcgcgtc gccgcctgcg attcgccctc tttttcctgt tgagcttatc gctttatgcg
     1861 tcgggcgcga gcctgttttc gcacgcgacg gtcgaagctg cgccgcgtgt gcagaagaag
     1921 aagggacagg cgaaagtgcg gcgcggaaac gtgcgacgcg cgcgtcgcgg aggcgtcgcg
     1981 gcgtcaacgt cgcgcacgcc gactgcgcgg cctgcgccgg tcgcggagcc gagcatcgga
     2041 gcgcaggagg acggcgacga gcgcgatgtg attgacgcgc gccagcagtg gtttatgatg
     2101 cagcgcgctt acccgttcga cacgcccccc gccggagcgc gcctcaaagc ctacctcgcg
     2161 cgcccgaagg acgcagggcg cggcaaagac ggaagctccg cttacgccgc cgtgcagacg
     2221 tggcgcagca tcggcccttc gccgaccacc ccggcctttc ccaacaactg gggcttgacg
     2281 agcgggcgac tcaacgccat cgccgtccat cccacgaacc cgcagatcat cctcctcgga
     2341 gcatccacgg gcggcatctg gcgttcgacc gacggcggcg caaacttcac tcccgtcggc
     2401 gacacgcagg ttgatctgtc cgtcggctca atcgccttct cgaagagtaa tccttcaatc
     2461 gtctatgcgg gcatgggcga cctcggcaac ggctatttcg gtacgggcgt cttgaagtcc
     2521 acggacaccg gggcgacttg gacgcgcagc agcaacaata cgcttccgcc gctcggcacg
     2581 accgccgaag tcgaagttga cccggccaac ccgaatcgcg tctacgtgct gcaagcctcg
     2641 cgcacgatga cgacttccta cgatattctc catcccgacg gcgcgctcta cagcagcggt
     2701 ttttatctct ccgaagacgg cggcgttaac tggcgtcgga cgctgcccgg caggccgcgc
     2761 gatctcgcag tgcatccgac gaattcgcag attctctatc tcggcatgaa ctccgtcact
     2821 gcgctcggca tcaacgcggg tctgtacaag tctctggacg gcggcgcaac gtggttgccc
     2881 gcacccgtct acactgcgcc caccggcatc agcacaagcg acgtgcgcgt cgccgtcacg
     2941 cctgccagcc cggagaacgt ctatgttatt gccggcacgc gaacagccct caccgtcacg
     3001 gtgagcaata acggcgggct gacgtggaca accaaaacca gcgctggagt agaccccggc
     3061 cagttcggct ataactccta catctacgcc gcgcccaacg acccgaacac catctatgtc
     3121 ggcacgcgcg acgttttcaa gagcaccaac ggcggcagca gttggactaa cctgacaaag
     3181 aattttgttc ccaaccccgc gcgcaccgac tgtagcttct gttacagccc gttcggttca
     3241 aactcgcacc cggaccagca cgccttcgcc ttcgcgccca acgatccgaa cacgctctac
     3301 ataggcaacg acggcggcat ctcgaaatcg tccgacggcg gcgtgtcgtt cgtctcgctc
     3361 aactcctcgc tcacgctcac gcagttcgta ggcatctcga tgcacccgac cgacccgaac
     3421 atctcctacg gcggcacaca ggacaacggc acgcagttac gcatccccgg cacgaacacc
     3481 tggcgagagt tcagcgccgg cgacggcggg cgcagcatcg tcaacgcgcc gaatccgagc
     3541 atcgtgttca ccacctacat ctacgggcgc gtcaaccgtt tcaccaacaa cgggcagacg
     3601 tttagcggca ccatcgccga gaacaccacc ttcggcgagc cgacgagcgg ccaccgcatt
     3661 gccttctacc cgccgatgac cggcaacggc gttgactcga cgatctattt cggcacctat
     3721 cgcctcttca cctgcaccaa ctgccacgac acgtcgagaa gccatccgaa cgctcccgca
     3781 aactggtctg cgcccggcga cactttcgat cagacgaaag gcaacggcga cgtgctgagc
     3841 gcaatcggcg tcgagcgcag agcgaacgca caggtcatct acacaggctc ggcgcaaggc
     3901 cgcatcatgg tcacggaaaa cggcggccag acgtggatag acatctctgc ggggatgccc
     3961 aatcgtttca tcgaacacat cacggtcgat ccgtcgaacc cggtggtggc ttatgtcacc
     4021 tacggcggct tcggcaccgg gcacgtcttc cgcacgacga acttcggcac gagctggacg
     4081 gacatcggcg gcatccccgg ccaaccgaac accatcccga acgtgcccgt cagcaccttt
     4141 ctcatcgacc cgacaacgcc ctccacgctc tatgccggga cggacatcgg cgtctttcgt
     4201 tcgaccgacc acggggcgac gtgggcgacc ttcaataaag ggatgcttcc ggcggtcgtc
     4261 accggattcg ccacgggcgc gaacggcgcg attcaactct caacctacgg gcgcggcgcg
     4321 tatgaactgg tcacgaccac gccgccgacc tacagcatcg gcgggcttgt gacactcggc
     4381 ggagttggtt tgagcggcgt cacgctgtcg ctcagcaacg ggcaggccgc gctgaccacg
     4441 accaccgacg ccagcggcaa ctacagcttc tccggcgtca cgggtggcga caactacgtc
     4501 ctcacgccgt cgaagccgaa cttcaatttc gcgccgcaga acatgaccta ctccaatctc
     4561 ggcgcgcacc agtctgcaaa cttcaacgcc gcctcggcgt gcctctattc cgtcgctccg
     4621 caagggctgc cgcgcttcgc cgcgagcggc ggggcgggca gtttccagat cactacgagc
     4681 gcgggttgcg cgtggacggc ttcgagcctc gtcccctgga tcacgatcac gaacgggagc
     4741 ggcacgggga acggcacggt cagcttcaac gtcgctccca acacgggcac ggcgcgcaac
     4801 gggcagatac ttcacaccaa cgattcgttc tacaacatca ctcaggaagc ctctccgtcg
     4861 cccgcgctcg tcgccttcgg agcggcaagc tacacgtttg gcgaagaggc cggacgcgcc
     4921 accatcaccg tcacacggac gggcaacacg tcaggcgcag tctcggtcga ggtcgctacc
     4981 gtagacgacc ctgcggccat cccctgcaac ccgacgacgc gcgaccagaa cggcaacctc
     5041 ttcccgcagg gcaaggcttt cgcgcgttgc gactatgcca ccagcatcga aacggtgacc
     5101 ttcgccgcgg gcgatacgca gccgaagagc gtctttgtcc cgctcgttga cgacgggcac
     5161 gtggagggag cggagacgtt ccagatcact ctgctcaatg cgtcgaacgc cgcgctcggc
     5221 acgctctcga ccacgacgct cacgctcacg gacaacgacg cgccgggaca ggccaacccg
     5281 atcctgcaaa cgccgctctt tgtcaggatg caatatctgg acttcctctc gcgcgaaccc
     5341 gaaccgggcg agccttggtc tggagtcttg aatcgctgcc cgcttccgtt caatctcgat
     5401 ccgctcaacg cgtcggcggg ctgcgaccgg atcatcgtct ccaagtcgtt ctacgaatcg
     5461 caggagtttc gtctgaaggg cttgtacacg ttcatctttt accgcgtggc cttcaatcgc
     5521 ctgccggagt atgcggagat catcccggac atgagaagcg tgacgggggc gacggctgcg
     5581 gaggtgtacg cgcgtcgcgc cctcttcccg tcgaacttcg cgcagcggca ggagtttcgc
     5641 tccgcctacg atgcgctctc acacacggcc tacgtcaacg cgctgctcga ccgttacggc
     5701 ttcgcctcca tcacgtcgcc cgacccgcag aaccccgaag gcgggacgaa ggtgactttg
     5761 agccgcgcgg atttgatcgc acggctcggc gcgcagggcg cgtccaactt gacgcgggcg
     5821 caggtcttgc gtgcgatagt cgaatcggac gagatgagag cagccgaatt cacacgcgcc
     5881 tacgtggcga tgcagtatta cggctacctg cgccggacgc cggaaccggg cggcttcaac
     5941 gcctggctca actacctgaa cgcgcacccg accgacgcgc gcacgatggt caacggcttt
     6001 gtgaactcag gcgagtatcg cgcgcggttc ggccagtagc agaaaagaga cgcagaggta
     6061 ggaacgcaga acgaggaact gaaaacagtt gctttcagtt cctcgttccg cgttcctact
     6121 tctgcgtctc tttagaaact gcggtcgtgg acgttgactt tagagacgga gaagacgacg
     6181 cgcatggtct gcgggcgacc atcacgataa gcggggacga aggcgggcgt ccggcgcaaa
     6241 gcctcctgca cctcgtcgag catttgcggg ttgcgcggcg cctgcactac gcccgcgagc
     6301 gacgcgctgc cgttgctgaa cacgtccgcg tagatcacca tgtcgtccgc gtcgtcgtcg
     6361 tcgctgctgg aaggcgtgcg gatgagcgcg gcgagcgcgc cgttcggatt gatcgtcggg
     6421 acgggcttcg tcacgtcgta gccctcgccc tcgaacagag acgtatcgag cgactcttcg
     6481 gaggcttgac gcgtggcaat cgccagttct cgcagcacgc gaaactgtgg gcgcagcgcg
     6541 ttgaccaccc cgacgaacag gatcatcgaa gccagcgcgc cgaccgtgta aggcatcaga
     6601 cgcggttcga gccagcgcat gaaacgcgtg gggagtgaac ccgtcggctg ctgcctgcgt
     6661 gcggcgcgtt cgattaagag cagttcgttg atggaaccgg cgagattgtg cggcacgcgc
     6721 gggcgttcaa gaccggcaag cgcacgcgtg atggaacgcg tgtgggctaa ttccagacgg
     6781 cacacgggac agacttcgag atgcgcgtcc acctgagcgc gcgaggcacg cgccagcacg
     6841 ccgtccagat acggcgaaaa acttttctgt gtctcctcgc aagtcataat tttaggggtc
     6901 ggaggttaga gtttagaggt tagaagagat cggttcttgt ttccattgat ctctgacctc
     6961 tagccgctga cctctgttcc cgaatggctg gccgaccccg gctcgctccc tcgctcaaga
     7021 aagcaacgcc gacagattat catttcacac gcccgctcga cgaacggcag caattcgtgg
     7081 cgcgcagtat tcgtcaacgg gtgttgctgg ccgcgtctta aataacctca cgcacgattg
     7141 ctgccttccg ctccgcacgc gacgaaactc aatccttcgt cgcttcccga cccattggca
     7201 cctcggatga tggatcaagc cggggcaggg cagccattcg gatgtcaaaa gagctgtgag
     7261 ctaaaagaag ctctgagccg tcagcaaaat catgctccca accttgttct aaacgccgtc
     7321 agcgtgctgc gcttcgcctg aaagctaacg gctgacaact cttcacaacg atcttcacaa
     7381 cgagccttcc aacttgcgcc gcagttccag acgaccgcgc gagagtctgg atttgactgt
     7441 gccgacgctg atctcaagcg tggcggcgac ctcttcgtag ctcaagcctt ccacgtcgcg
     7501 caggacgacg gcttcgcggt acgggcgcgc gagcgtgcgg acggcggcga gtaaaatctc
     7561 ttcgcgttcg tgcgcgagcg tgcgttcttc ggggtcgtca gcccgctcgt ctgcgaggcg
     7621 cgtggcgagc gagagttcgt gcgtctcgtc tgtggcgtcg agcgagacgg tcacgttgcg
     7681 tcttctgcgt cgccaccaac gccagcgatt gcgcgcctga ttgacggcga tgcggtagag
     7741 ccacgtgcgc aaatcggcgt cgccgcgaaa gtgtgcgatg ctgcggaagg cttgcaggaa
     7801 agtctcctgc gtcaaatcgc gcgcctcttc ggggtcttcc gtcaggcgat agagcagcgc
     7861 gtaaatatct gccgagcgtt cgttgacgag atgatcgaag gccgccgcct cgcccgcgcg
     7921 taatctttca atgaactgta gttccggact cggacgcgac tccgccgact ccgccgcgac
     7981 ggcgggatgc tccaattcgg cttgcccgcg gagggcgatt ggctctccga agctcacaaa
     8041 cgtcctccag cgtgaaccgg cgactcatgc attggccgct cgccgtgcac tcgacagatt
     8101 agacaccgcc ctatggcggc ttgttcccga aaaattttac tcgttttcac tcgacgcata
     8161 agctgctcgg aggggacggg cgcgaagcgt agcacgcacc cccgtaacgg tcaataaagt
     8221 ctttgccagc taggatgttg agccgcctga atggttgctt gacaaccccg cacgacggtc
     8281 gttagagtga atcgtttcgc cctaactact gaatttagaa cgcgcgcacc tgcgcgacat
     8341 tgcaagagaa aaaacgtggc tccatccaga aaaaaacatg cgcgcgaagt ccagcgcgat
     8401 aaatttcgcg aaaagacact ggcacaattt gatcgcgtcg gcgaccgcct cgaaggcaaa
     8461 ggccgcacgc tcctctacgc tctcgccgga ctcgtcggcc tcgccttgct ggcgggcgtc
     8521 tattcctggt ggagcgggcg caaggccgag gaggccaccc gcgctctcgg tcgcgtcacg
     8581 gagatcgtca acgccaccgt cacgccctcg cccatgcccg gctcaaccgg cctgagtttt
     8641 acgagcgaga aagagcgcgc gcagcgcgtc gtcgaggaag cacagaaggt cgaacaaaaa
     8701 tacggcgatc cttacaagtc gctcgcacac tactttcgcg cggccaatct cctgacggtt
     8761 gatcgcagca aaggtctgag cgaactcgaa gcgttgacga agaacggcga cgcggaggtg
     8821 tcggcacgcg cgaagtttgc gctggcgcag gcgagggaag ccgacgggca gcacgacgcc
     8881 gcagccgcgc tctacctcga actcgtacgc gcgcaaaatc cagccgtccc ggttgacacg
     8941 gcgaacctgc gcctcgcctc catctatgaa aagcagggca agaagagtga ggccgcagac
     9001 attctcttcc agatcgccaa gacggcgcgc gaggcaaaag acaaggacgg caaaccctcc
     9061 acgcaatccg ccgccgcacg cgaagccgcg caaaaactgg aacgcctcga ccctgcgcgc
     9121 cacgcgcaac tcccagccga acctgctccc gcaacaaatc ttccattcta aaaacagagg
     9181 tcagagattg gagagatcag tttaaaagca caaactgatc tctctaatct ctgacctcca
     9241 gccttcgacc tctatcctca atggacaatc tcacgcacac gctggtcggg ttgacggctg
     9301 cgaaagcggg gttggaacgc ctctcgccgg gggcgaccgc cgtgtgcatg ctcgccgcca
     9361 acgcgccgga cgcggatatc gtcgcgacct tcggcggaag ttggttctat ctcaaacatc
     9421 atcgcggtat cacccattca atcgtcggga cgctcaccat cgcgttactg cttcccctgc
     9481 tgttctatgc cggggatcgc ctcatcgcgc ggcttcgtgc gcgcccgccg cgcgtacggc
     9541 tgcgcgggct ggttatcgct tcgctgctcc tcagcgcgtc gcaccctttg atggactgga
     9601 cgaataacta tggcctgcgc cctctgttgc cctggagcgg gcgttggttt tacggcgacc
     9661 tcgtctacat catagacccg tggctctggc tgagtctggg cggggcgtcg tttctgttga
     9721 ctgctaagac gcgctggcga agcgtcgcgt ggggcgcgct cgcgctgctg gcgacggtcg
     9781 ccttcgtcgt cttgcccctg cgggtgggcg agacttaccc gcacacatcg cgcgctttgt
     9841 ggctcgtgtg gctcgcctgc ctgatcgtcg cgcatcgcat gaaagtcgcg ccgcgtctcg
     9901 gaagttccat cgctctggcc gcgctcgcgt tcgtcgtcct ctactggggc gcgctcggct
     9961 tcgtgcacgg gcacgcagtc gaacgcgcgc gggcggtcgc ggaggcggtg gcggcgcgcg
    10021 ggggagagtc tgtgctgcgt gtcgcggcga tgccgtctct ggccgaccct tcgagttggc
    10081 gcgtgctcgc ggagacggat cgcgccactt accgttttaa catctccctg ctcgcgcgcg
    10141 tcgaaacgga agccgccgcg cccgctgcgc tcgcgcgctt cgagaagccg cagggtgaag
    10201 ccgccgaact cgccgcgcgt gccgccgtag acgagcgcgc cgaaatcttt ctcgacttcg
    10261 cgcgctttcc cgccgtccgc gtcgagacgc aaaactgcgc cgaagaactg ctcgtgcaat
    10321 ttgccgacct gcgtttcacc gaaccgggca gcggacgccg cgctggcaac ttcagtttag
    10381 aagttcccgt ccgccccgcg cgctgagcgt tcgtcgtcgt ttggaagcgc acgctaaatt
    10441 ccggctgcgt ataattttgt gagtcccgtc cttcgttctc gcctgcccgc ctgttcagcc
    10501 ttttctacgc cactgggtta taatcaatct cgcccgacct cgatctaatc cttagtgttt
    10561 agtgagtcgt gtctatgaaa ttcgcgtcgc acgaagttgg aaattcgtct ctgacggcaa
    10621 gagagtctcc gggggaagcc tccgatccgc cagtgacgga agcgagcgac gggcgtttcc
    10681 tcacctttgc cgaaagcgca gccgacgcca tcatcgtcat tgacgccgcc agcgacatcc
    10741 gcttcatcaa tcgcgccgcc gagaagattt tcggctatcc cgtacatgaa ctgctcggtc
    10801 ggaatctgac cattctgatg cccggctacc tgcgccacgt tcatcgcgaa gggttggcgc
    10861 aatacctctc gacgggcgtg ccgcacatca attggagcag cgtcgaactt cccggcctgc
    10921 accgcgacgg gcgcgaggtc gcgctcgaaa tctctttcgg tgcgttcagc gaaaacggcg
    10981 tgcgctattt cacgggcatc gcgcgcgaca tcaccgagcg caagcgacag aaggaggctc
    11041 aacgctttct cgtcgaagcg agcgcgctcc tggccgcgtc gctcgattat gaaaccaccc
    11101 tgcaatcggt cgcacatttg agcgtcccgt tcctcgccga ttggtgcatc gtctatgtgg
    11161 tggacgacga tgcggccata cgtcccatcg ccgtcatgaa cgacgacgct gcgcaggtcg
    11221 agttggtctg ggaacttgag cggcgcgaga accagaaccc ggaggctttg acgggcgtgc
    11281 cgaaagcgat tcgcaccggc cagtccgaac tctacgcgga cattcccacg gctgccgaaa
    11341 cgtctgcggc gagcgacgcg ggaaggtttc gcgtcgcacg tcaactcgat ttgaagtcgg
    11401 cgatgatcgt gccgctcgtc gctcgtggcc gcacgctcgg cgcgattacg tttgccaccg
    11461 ccgtttcggg acgccgctat gccccctccg atctcgcctt cgccgaagac ctcgcgcagc
    11521 gcgccgcctt cgcggttgat aacgcgcgcc tttatcgaga agcgcaggaa gccaaccgcc
    11581 tgaaagacga atttctcgcc acgctctcgc acgaactgcg cacaccgctc acctccattc
    11641 tcggttggac gaatctgctt cgctccgtca agtttgacga aagcactacc gagcgcgcac
    11701 tcgaaaccat cgaacgcaac gcccgttcac agaagcaact catagatgat ctgctcgatg
    11761 cctcgcgcat catcacaggc aaactccgcc tcgaattgaa gccgacggaa ctcaaatcgg
    11821 tggtcgaatc ggcgtgtcat gcggcgcgtc cggcggcgga tgcgaagggc atcgaattga
    11881 gccttgcgct cgaaggcgcg tcgagcatcg tggcgggcga tgcggaacgc ttgcagcagg
    11941 tggtgtggaa tcttctctcg aacgcggtga agtttacgcc ggagggcggg cgcgtcgaag
    12001 tgcgtctgga gcgcgtgggg acgcaggcgg agatttccgt aagcgatacg gggacgggca
    12061 tcaaagcgga gtttctcccg ttcgtcttcg agcgtttccg gcaggccgac gggagcacga
    12121 cgcggacgca cggcgggctt ggactcggcc tcgccatcgt ccgccatctc gtcgaactgc
    12181 acggcggaac ggtggtcgcc gaaagcacga gcgcgggcgc gactttcaag gtgagcctgc
    12241 cgctcatcga atacgcgaag cgcggcgaga ctgcgacggc gggcgagttg aggtttgaag
    12301 gcgtgggtga acccaccggg cgacaggcgt tgctacgcgg tctacacata ctgatcgtgg
    12361 aggatgaacc ggacacgcgc gtgctcttga agaaagctct ggaacgctac ggcgcacagg
    12421 tggagacgcg cgagttggcg gaagaggctc tggacgcgct cgccgcctct gcgttcgatg
    12481 tcgtcctgag cgacatcgcc atgcccggag aggacggcta ttcgttcatc gaaaagatca
    12541 gggcgcgcgg ctacacgact cccgccatcg ccctcacggc ctacgcacgc gaagaagaca
    12601 ggcaactcgc actcgcctca ggcttccaac ttcatctcgc caagcccgtc gccccgaaag
    12661 aactggccgc cgccgtcgcg gatgtcgtcg ggcgcacgtc gaatgtctga agcggcgcga
    12721 gaggccgaac agttcattcc ttacctctta atctcatcca gagccgcgct caaacgttcg
    12781 agtccgccgc ttaacattcc ggtatcgccg ccgatgctga ggcggaaatg ttcgggcatc
    12841 tcgaaaaatc tccccggcac tatcgaggtt tcatacttct cgcgcagcaa cgcgcagagg
    12901 tcttcgacgc tgccgcgccg gagacgcggg aacgcgatca tgccgtgcgt gatggggaag
    12961 gcgtcgagat cgtcccgccg cgtctctagg aagtcgttgg cgaggaggcg gtttcgttcg
    13021 agcagcgcgc gggagtgagc ggcgatctcc gggagacgcg cgaaggcgag gcaagcgagc
    13081 cgttcggagg cgtgcggttg aactgcgccg aagagatcgt tgagtcgcca catacgtgcg
    13141 gcgagcgcgg gcgcggcgag tatccagccg caacgcaggc cgctgaggcc gtaaactttc
    13201 gtgagactgc tcgtggtgat gaacaattcg ccgagatgaa aggctgactg cggcgcgtcc
    13261 gagaaggccg cgtcgagata gacttcatct acgagcacgc gcgcgcccac gctatgggcg
    13321 atctctccga gttgtgaaat cgtctcgtcg tgctcgtagg cgttcgaagg gttgtgcatg
    13381 ttcgtcacga cgatgagctt cgtgcgcggc gtgacggcgc gtgcaacttc atctggatca
    13441 atacggaatc gagcttccgt atggcgcgtg aatcttgtga ccttcgctcc gagatagagt
    13501 gcggcggaga ggagcagttc ataggtcggc tgttcgatga gaacttcgtc gccgggttcg
    13561 atgagagtcg ccagcgcgat aaagttcgcc atggaagtgc cgttggcggc gacaaccgaa
    13621 tcggctttca ctctgcattt cgcggcgatg gcctgttgca gcggttggta gccgtaggtg
    13681 ctcaccccgc tcagttcgag gtcggagagt tcgacgcgca attgttcgag cgtgtaaggt
    13741 ctgactccac tcgtcgcgag attgtaggtc gcctgcgatt gagtcttcgc ccagtgcatg
    13801 taggccgaac tcatcacgcg cggttgcgtt tctcctgtgc tcatcagcga tctctccttt
    13861 gttgtctcag ccagtagtag caggcgggga cgcccgcgag cacaatcgcg aaaccgatga
    13921 ggctgttgac cgggtattta tagacggtgc tcaggacgac gagccagcag gccgcgacga
    13981 agagcgcggt cgtgtatgga tggccgggga ctttaaacgt cgcctcaccc gcagggtcgc
    14041 ggcggcgaaa gatgaagacg gagagtccgg tcagcccgaa ccagatgaag tcaaccgaga
    14101 cgacgtagtt gagaatctgt ccatacttgc ctgagagcgc gacgatgatc gcgagcgcgc
    14161 cctgcaaagc gatggcgaag acgggcacgc gcgtccgcgt gtggacttgt gcgatgcggt
    14221 ggaagaacaa tccatcttcg gccatggcga aatagacgcg cggcgtggtg agcagggatt
    14281 ggctgaggaa gccaagcgtc gagatgctga tgccgagcgc gatgagggtc gcgccgcgtt
    14341 cgccgagcgc gcgacgcatg acggcggtag cgggcgtgtt ggtctgcgcc aacccttccg
    14401 cgccgagcgc atagacacag acgaagctga cggcgacgta gagcaggatc acgcccgcca
    14461 cgccgaacag cagcccgcgc gccaggtccc tgcgcgggtc gcgcatctcg cccgcgatga
    14521 aactcgtcgt ctgccagccg ccgtaagcga acatga
//
LOCUS       DASWNS010000022        16329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_337970, whole genome shotgun sequence.
ACCESSION   DASWNS010000022 DASWNS010000000
VERSION     DASWNS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16329
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337970"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..348)
                     /locus_tag="VGB76_00555"
     CDS             complement(46..348)
                     /locus_tag="VGB76_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEY0097414.1"
                     /translation="MSEHIVSKKVYFVIFGALMALTVLTVVAANYDLGSEKINTIVAL
                     AIAVTKAVLVVLYFMHVRYSSRLTWVVVAGGFLWLVIMIGLTLSDYLSRGLLTYPR"
     gene            complement(363..1094)
                     /locus_tag="VGB76_00560"
     CDS             complement(363..1094)
                     /locus_tag="VGB76_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEY0097415.1"
                     /translation="MAHAETLDAHNQEEATHHHASSNLAHHFDNLEQQREAGSLGMWL
                     FLVTEIMFFGGLFTGYIIYRMQHPQAFAIASSTLNWKLGALNTVVLIVSSLTMALAVY
                     FAQMGSRRMQIIFLVVTMLLGATFLVVKAFEYADKFEHHHFPGLNFQWNEAVPAGVST
                     HHIQMFYWIYFAMTGLHALHMIIGIGAMLFLIYYAYRGRYTPEYHAPVELTGLYWHFV
                     DIVWIFLFPLLYLLGAHLAGGGGAH"
     gene            complement(1137..2879)
                     /locus_tag="VGB76_00565"
     CDS             complement(1137..2879)
                     /locus_tag="VGB76_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY0097416.1"
                     /translation="MSSHDPSIPLPPPNAPHAPTGLDALGSRTRFAHDNYITANYGIK
                     SWLLTKDHKRIALLYLASITVFFILGGIYALVIRLELLTPTGNIVQPMTYNKLFTQHG
                     IIMIFFFLIPSIPAVLGNFLVPLMIGAKDLAFPKLNLLSWYLYLAGAVFTIWALLNGG
                     IDTGWTFYTPYSTTFSNSYVVAAGLGIFINGFSSILTGLNFIVTIHTMRAPGMTWFRL
                     PLFIWSHYATSLIMVLGTPVIAITILLLALERIVKIGIFDPRLGGDPVLFQHFFWFYS
                     HPAVYIMILPAMGVVSELISTHSRKNIFGYSFVAFSSLAIAVFGFLVWGHHLFTSSQS
                     VYAGMIFSLVSFAVAIPSAVKVFNWTATLYKGSISYDTPMLYALGFIGLFTIGGLTGL
                     FLAALGMDVHLTDTYFIVAHFHYIMVGGALMGYLGGLHNWWPKITGRMYPEGWGRFGA
                     LVIFVGFNLTFFPQFLLGYMGMPRRYHAYAEEFQVLNVFSTAGASILGFGLLIPGIYF
                     IWSMRYGKFAPANPWAATGLEWRTVSPPPTENFAATPVVTWEAYEYRPMEEMDVIGPD
                     KRNTAQPAHGEALT"
     gene            complement(2979..4154)
                     /gene="coxB"
                     /locus_tag="VGB76_00570"
     CDS             complement(2979..4154)
                     /gene="coxB"
                     /locus_tag="VGB76_00570"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY0097417.1"
                     /translation="MPRLPFTPEQASTVSTEVDALYTYLVAITLFFSVLVTVLIFYFA
                     VKYRRRDANELPQPIAGSIKLETMWTVIPFLIAMTIFVWGASLYFTLYRPPKDAIEIF
                     VVGKQWMWKFQHTEGQREINELHVPVGAKVKLTMTTEDVIHSFAVPAFRIRSDVVPGR
                     YTFAWFEATKTGRYHLFCTEFCGTNHSGMTGWVEVMEPTAYQAWLAGGGSDSPAAQGD
                     KLFQSLGCATCHRTDTQGRGPVLTGVFGKPQRMQNGEDVLADEAYIRESIVNPTAKIV
                     AGFQPIMPAYQGQVNEEQLLQLIAYIRSLGAPQQQQGGSTMTNAGEGQQEPSTTTGIN
                     PDAQRSNPLDSTSPRPGGAGAPTGGLSPTSQRPSTRTGGTTTTTGGQTGGNSNQRQQ"
     gene            complement(4165..5118)
                     /locus_tag="VGB76_00575"
     CDS             complement(4165..5118)
                     /locus_tag="VGB76_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0097418.1"
                     /translation="MNKVISRNRVVGERSRAPWLARGVACLLLLAVCCSPLTVLGQYA
                     QPPQGGVSSNDVPLPLKEIGIEQRLGGQVPLELKFRDETGQHVRLGDYFGKRPVFISL
                     VYYNCPKLCNLVLNGLVGGLRTLPFTVGKEFDVVTVSFDPSEGASDAVKKKEVVMHDY
                     GRQGDASVAANWHFLTGDKTSIDALADAVGFKYSFDTATNQYAHASGVMLATPEGKLS
                     HYFYGIEYAPRDLKFGLVEASEGKIGSVVDKLTLYCYHYDPTTGKYAPLIMNIMRLTG
                     ILTVIGLVVLISLLHRRRRRREEASPSPPEGLSTGEAGGIA"
     gene            complement(5148..5765)
                     /locus_tag="VGB76_00580"
     CDS             complement(5148..5765)
                     /locus_tag="VGB76_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097419.1"
                     /translation="MGIFGQHGNGKGKMHLTETPDVSHITNPDVMHEESDVSVRGVGT
                     FVGLLFLGLIIVCGLMLGLFKLLEAYATSKEIAPAPMARKAEERLPPPGVPRLQGAPD
                     HRSLDDPNLIFEKKEPTLEWDALKAKFKYDLETAAEVNPGTGMWRIPIADAKKKLLEK
                     GLPVRQQSPPQQQQQQQQGGGAASRDGLHLPTAASAGRQTERRDQ"
     gene            complement(5852..7123)
                     /locus_tag="VGB76_00585"
     CDS             complement(5852..7123)
                     /locus_tag="VGB76_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097420.1"
                     /translation="MNAPNIAATTATTYNAPPNIERLGGRALVVGLVALVLCALGAFV
                     NLAGFWRAYLVGYVFWTGVSVGSLAIIMLHHLSGGGWGVVIRRPLEAATRTLPVMFVL
                     FIPLAVAVLTHGLYPWTVPEERDLEIIKHKAIYLNVPFFLGRTVFYFAMWWLLQHFLS
                     KWSLEQDTAKDPRERRRLKDKLQNLSGPGILLFGLTVTFAAVDWVMSLEPEWFSTIYG
                     LLIMVGWGVTALAFAIVTTVILSKDERLAHVYQPRHFHDHGKLLLAFVMLWAYFSFSQ
                     FLIIWSGNLPEEIPWYLRRLYGGWGYVAVALVILHFALPFVLLLSRDLKRRARALVLV
                     ALLVLFMRVVDLFWTIAPSALAMPHHGGGAASHAGGFPDVVAYALNFVTPILIGGIWL
                     WWYTRELRSRPLLPLGDEGLANALAEPSGHH"
     gene            complement(7120..8010)
                     /locus_tag="VGB76_00590"
     CDS             complement(7120..8010)
                     /locus_tag="VGB76_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0097421.1"
                     /translation="MSNTEPRRGQRSEAGGQSPMFGRRAALCLLLAACCLLALGCRQD
                     MQDQPRYEAYEPSKSFGDGQSSRPFVEGTVARGYRDINTSYYTGKQASGATTGGAQGL
                     MNGSGNALQATMANAQGGSAGAQAAGGGDVNVRGQNVGANVPNGAGGGTAGQSGTPGS
                     INEFPFPIAEKDLDRGQERFEIYCAMCHGATGYGDGMIVRRGFRHPPSLHDDRLRGET
                     VGHFFDVITNGWGAMPDYSQQIPVEDRWKIIAYIRALQLSQNTPVAEVPEHLRNQRKP
                     TPGTPGTQGGHQGAGGERTP"
     gene            complement(7997..8542)
                     /locus_tag="VGB76_00595"
     CDS             complement(7997..8542)
                     /locus_tag="VGB76_00595"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEY0097422.1"
                     /translation="MKKPTRPAIYGVMAEFDNPTDVVAATRRAYEEGYRRMDAYSPYP
                     IEALSEAVGPHKERLPLIVLIGGIVGLIGGYAMQYYVSVWNYPINVGGKPLHSWPAFI
                     PITFETTVLGAAMAAIFGMLALNGLPEPYHPVFNAPNFALATRDRFFLVIESRDPKFD
                     REATSGFLRSLGAQEVADVEH"
     gene            complement(8675..10078)
                     /gene="nrfD"
                     /locus_tag="VGB76_00600"
     CDS             complement(8675..10078)
                     /gene="nrfD"
                     /locus_tag="VGB76_00600"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY0097423.1"
                     /translation="MASRVDEPHDDLIHVTPNPVIEPGHTAASVTDKISALVLKRKTP
                     LGWYIGFFISFSLANVLMFTIAYLVTTGIGIWGNNWPVGWAFDIINFVWWIGIGHAGT
                     LISAILLLLRQEWRTSINRFAEAMTLFAVACAGLFPLLHTGRPWLAYWMLPYPNTMNI
                     YPQFRSPLMWDVFAVSTYASVSALFWYVGLIPDFATLRDRAKSGVFKVIYGMLALGWR
                     GSARHWHRYETAYLLLAGLATPLVLSVHTIVSFDFAVSQLPGWHATIFPPYFVAGAIY
                     AGFAMVLLLTIPLRKFYGLEDFITLRHLRNSAKVMLATGLIVGYGYMMEAFMAWYSAS
                     EYEGFMLKNRIWTGPYWWAYALLIITNIIIPQILWSRRARHNTVLLFIVALIISVGMW
                     LERFVIIVTSLHRDYLPSSWAMYRPTIFDWSMFIGTIGLFFTLLLLFIRFLPVISIFE
                     MRTILPGAAPKAVASDE"
     gene            complement(10085..13516)
                     /locus_tag="VGB76_00605"
     CDS             complement(10085..13516)
                     /locus_tag="VGB76_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEY0097424.1"
                     /translation="MHIKESFPELYNIRRPVKREEIRSRLEEKTGREYWRSLEELADS
                     GQFEDFLHAEFPRQAGEWDDEVGRRTFLKLMGASLALAGLTSCSYQPPETVVPYVNQP
                     PELVPGKPLFFATAMPFPGGATGLLVRSNEGRPTHAEGNPDHPTNGTPERPVGQRVAA
                     TDVFSIASILSLYDPDRSETITHRGEIRPYTAFLAEIRTRLGEMNGRQGAGLRFLTET
                     VTSPTMAAQLRAILAAFPAAKWHQYEPAGTNNASLGAQLAFGQPVNTIYRFDRAERVL
                     SLDSDFLTCGTANLRYARDYAAKRRVLAGKTDMNRLYVVETTPTSTGACADHRFSIKP
                     SQMEMFARALASAVGAPGAPTDGAQFQNVAHAGHIFAIAKDLQAHRGRSIVVAGEHQP
                     PAVHALAHAMNQSLGNVGQTVIHTDPIEAQPLDQVASLRELVGDIEAGRVEMLVIVGG
                     NPVYNAPVDLAFTKERLDKIRTPDGKDGLRVHLGLYDDETAELCHWHIPETHFLEAWS
                     DTRAHDGTVSIIQPLIAPLYKGRSVHEFLAAFTDAPERSGYDIVRDYWRTQRLESLVA
                     AASASQAATQAGSDNTHGQGGTTAQNIPTAEHGANPNASATPGAAPNAIPSPTPSATP
                     TATPSPATNATRGAAAAPANFDAAWRAVVHDGVVPGTAAAVRNVTVRGDWSASATTAQ
                     PTPAGGFEIVFRPDPNIYDGRFANNGWLQELPKPLTKLTWDNAAYMSLATARRMFGLN
                     ENDYTFKGREFNAPLVDLRYRGQTVRAPVLIVPGQPDDVVTVHLGYGRTRAGKVGTST
                     ADQPLGFNAYRLRTSDAMWQGAGLEVAPTGEEYLLAVTQLHFNMEGRDIVRSNTLTEY
                     LKGGGDSSREVHGGASVATHEGAAEVVGKEMHKEPGPNDTLYPNYEYTGYKWGMAVDI
                     ASCVGCQACVVACQSENNIPIVGKDQVSRSRHMHWLRVDTYFKGLAENPEGAHFMPIP
                     CMHCEVAPCEPVCPVHATVHSAEGTNDMVYNRCIGTRYCSNNCPYKVRRFNFLLYQDF
                     NTPSLKMMRNPDVTVRSRGVMEKCTYCIQRISYARIEAEKEERRVRDGEIVTACQSAC
                     PTEAIIFGDLNDQQSRVAQMQHDKRNYSLLAELNTRPRTTYLSAMRNPNEEIHGA"
     gene            complement(13544..14227)
                     /locus_tag="VGB76_00610"
     CDS             complement(13544..14227)
                     /locus_tag="VGB76_00610"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY0097425.1"
                     /translation="MPQIFHRSTNTLAKLSIFGSIFVLLFVTWAVAEINRSSWNTGAF
                     DEREQPVQFSHKHHTGDDGIDCRYCHTSVETSSSAGLPSTKTCMNCHSQLWNDSPYLE
                     PVRASWRDNKPIEWTRVHDLPDYVYFNHSIHVNKGVGCSSCHGRVDEMPLVYQAASLQ
                     MEWCLECHRQPERYLREKSEVFNMDYQQENPLAENKTAAQLQKGRELLVKHKIRSKSI
                     LESCSTCHR"
     gene            14711..>16329
                     /locus_tag="VGB76_00615"
     CDS             14711..>16329
                     /locus_tag="VGB76_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0097426.1"
                     /translation="MKQRISRAFIVLSFVLLAVCGAAAKDTWTSIKSKNFTVVGNAGE
                     GEMRKVTMRLEQFRQAVSVLFANAKVETRVPTTVVIFKSHGSFKPFKPKYKGKTRENV
                     GGYFINGGDLNYIVLTTETHSGISPYEIIFHEYEHFILANSMRHAPAWLHEGLAEFVS
                     TLETSDNEQKVRLGTPIAHHVYTLRQNNPIPLKTFFTVDHRSPQYNENDKVGIFYAQS
                     WALIHYLMLGNDGARRPQLTRFINGLNGDLPIEENFRQSFGTDYAKLEHELRGYLRKE
                     AYPVLDFKLQKPLELSKEAVTTTLTEAQVEYHLGDLLLRARRFDEATERLEKSVALDA
                     GFAPPRVSLGIARLRQNRPGEAQKILRGALALDPRNPLAHYHYASALGAEDRLEEAVA
                     AYREAIALKPDMAGAHAELGRTYRNLRRNEEALEAYNQAIRHSPDSPYFYQSRAYINL
                     QLGRGNGASFDARAYLHLQGWRDDSAPYLALAAYFGFRQKNRAADAARMLEEAAAKTD
                     AAAWPRPVFKYLAGELTAEELIKQANNNDELT"
BASE COUNT         3204 a         4965 c         5117 g         3043 t
ORIGIN      
        1 tcgggcttct ctctcacaaa cgggggcagg cgtgtctcgc ccgcttcatc tcgggtaagt
       61 gagcagacct ctcgataagt agtcgctcaa agtgaggccg atcatgatca cgagccacag
      121 gaagccgccc gccacgacga cccacgtgag ccgcgaactg taacgcacgt gcataaagta
      181 gagcacgacg agcacggcct tcgtcacggc gatggcgagc gcgacgatgg tgttgatctt
      241 ctctgagccg aggtcgtaat ttgcggcgac gacggtgaga acggttaacg ccatcagcgc
      301 gccgaagatt acgaagtaaa cttttttcga gacgatatgc tcagacataa cttcttcacc
      361 ctctaatgag cgccgccgcc cccggcgagg tgcgcgccca gcaaatagag cagcgggaac
      421 aggaaaatcc agacgatatc gacgaagtgc cagtacaagc ccgtcagttc gacgggcgcg
      481 tgatattcgg gcgtgtagcg tccgcgatac gcgtaataga tcaggaacag catggcgccg
      541 atgccgatga tcatgtggag cgcgtgcaag cccgtcatgg cgaagtaaat ccagtagaac
      601 atctgtatat ggtgcgtcga gacgcctgcg ggcacggcct cgttccactg gaaattgagt
      661 cccggaaaat gatgatgctc gaacttgtct gcgtactcga aagccttgac gacgaggaag
      721 gtcgcgccta gcagcatcgt cacgacgagg aagatgatct gcatgcgccg actgcccatc
      781 tgcgcgaagt agacggcgag cgccatggtc aaactgctga cgatgagaac gaccgtgttc
      841 aaagcgccga gtttccagtt gagcgtgctg ctggcgatgg cgaaggcttg cgggtgctgc
      901 atccggtaga tgatgtagcc ggtaaagagg ccgccgaaga acatgatctc cgtgacgagg
      961 aagagccaca tgccgaggct gcccgcctcg cgctgctgtt cgagattgtc gaagtggtgc
     1021 gcgagattgc tggaagcgtg atgatgcgtc gcttcctcct ggttgtgcgc gtccaatgtt
     1081 tctgcgtgtg ccatgtgctt ctttcttccc tcccggctgt caggtcaaaa cggctgttag
     1141 gtcagagcct cgccgtgagc gggctgcgcg gtgttgcgct tgtcggggcc gatcacgtcc
     1201 atctcttcca tcggcctgta ctcgtaagcc tcccacgtga ccacgggcgt cgcagcgaag
     1261 ttctccgtcg gcggcgggct gacggtgcgc cattccagac cggtcgccgc ccacgggttc
     1321 gccggggcaa acttgccgta acgcatcgac cagatgaaat agatgcccgg aatcaacaac
     1381 ccgaagccga gaatggacgc gcccgccgtc gagaagacgt tcaagacttg aaactcttcg
     1441 gcgtaggcgt ggtagcggcg cggcatgccc atgtagccga ggagaaactg cgggaagaag
     1501 gtcaggttga agccgacgaa aatgacgagc gcgccgaagc gtccccagcc ttccgggtac
     1561 atgcgcccgg taatcttcgg ccaccagtta tgcaatccgc cgaggtagcc catcagcgcg
     1621 ccgccgacca tgatgtaatg gaagtgcgcg acgatgaaat aggtgtcggt caaatgcacg
     1681 tccatgccga gcgcggcgag gaagaggccg gtgagtccgc cgatggtgaa cagcccgatg
     1741 aagccgagcg cgtagagcat cggcgtgtcg taggagatcg accccttgta gagcgtggcc
     1801 gtccagttga acactttcac cgccgaaggg atcgcgacgg cgaaggagac gagcgaaaag
     1861 atcatcccgg catagaccga ctggctcgaa gtgaacaggt gatgtcccca cacgaggaag
     1921 ccgaagacgg cgatggcgag actcgaaaac gcgacgaacg aatagccgaa aatgttcttg
     1981 cgcgagtggg tcgagatgag ttcgctgacc acgcccatcg cgggcaggat catgatgtag
     2041 accgccgggt gcgagtagaa ccagaagaag tgctggaaga ggactggatc gccgccgagt
     2101 cgcggatcga agatgccgat cttgacgatg cgctccagag cgaggagcag gatcgtgatg
     2161 gcgatgacgg gcgtgcccaa gaccatgatc aaactcgtcg catagtgcga ccagatgaag
     2221 agcggcaggc gaaaccaggt catgccggga gcgcgcatcg tgtgaatggt gacgatgaag
     2281 tttaagcccg tcaggatcga ggagaaaccg ttgatgaaga tgcctagtcc ggcggctacc
     2341 acgtaagagt tcgagaaggt ggtggagtag ggcgtgtaga acgtccagcc cgtgtcaatg
     2401 ccgccgttca ggagcgccca gatggtgaag accgcgcccg ccagatagag ataccaactg
     2461 agcaggttga gtttcggaaa ggccaaatcc ttcgccccga tcatgagcgg gacgaggaag
     2521 tttcccaata ccgccggaat ggacgggatc aggaagaaga agatcatgat gatgccgtgc
     2581 tgcgtgaaca gcttgttgta ggtcatcggc tggacgatgt tgccggtggg cgtcaggagt
     2641 tccagacgga tgacgagcgc gtagatgccg ccgaggatga agaagaccgt gatcgaggcc
     2701 aggtagagca gcgcgatgcg cttgtgatcc ttcgtcaaca gccacgactt gatgccgtag
     2761 ttggcggtga tgtagttgtc gtgcgcgaat ctggtgcgcg aacccagcgc gtccaaaccc
     2821 gtcggcgcgt gcggcgcgtt cggcgggggc agcgggattg acggatcgtg tgaactcata
     2881 cttcgtttaa ccttctgtct tcaacctgtg aactcttctc gactgcctcg ttccggctga
     2941 acgcagccgc tctgccggat gcgcctcacg gcgtctctct attgctgccg ctgattactg
     3001 ttgccgccgg tctgcccgcc agtcgtggtg gttgtgccgc ccgtcctcgt gctcggacgc
     3061 tgcgacgtgg gcgacaagcc tccggtgggc gcgcccgcgc cgccggggcg cggcgaagtg
     3121 gagtcgagcg gattcgagcg ttgcgcgtcc gggttgatgc cggtcgtcgt cgaaggctct
     3181 tgctgcccct ctccggcgtt cgtcatggtc gaaccgccct gctgttgctg cggcgcgccg
     3241 agcgagcgta tgtaagcgat gagttgcagc aactgctcct cgttgacctg cccctgatag
     3301 gcaggcatga tcggctggaa gcccgccacg atcttcgccg tcgggttgac gatggactcg
     3361 cgtatgtacg cttcgtcggc caacacgtcc tcgccgttct gcatccgctg cggtttgccg
     3421 aaaacgcccg tcagcaccgg accgcgcccc tgtgtgtccg tgcgatgaca cgtggcgcag
     3481 cctaaggact ggaaaagttt atcgccctgc gccgcagggg agtctgaccc gccgcccgcg
     3541 agccacgcct gataggccgt cggctccatc acctcgaccc agcccgtcat gcccgaatgg
     3601 ttcgtgccgc agaactccgt gcagaacagg tgataacgcc ccgtcttcgt cgcctcgaac
     3661 caggcgaagg tgtagcgacc cggaaccacg tcggagcgga tgcggaaagc gggcacggcg
     3721 aacgagtgaa tgacatcttc ggtcgtcatc gtcaatttca ctttcgcgcc gacgggcacg
     3781 tgcagttcgt tgatctcgcg ctgcccttcc gtgtgctgga atttccacat ccattgtttg
     3841 ccgacgacga agatttcgat ggcgtccttc ggcgggcggt agagcgtgaa atagaggctc
     3901 gcgccccaga cgaagatcgt catggcgatg aggaacggga tcacggtcca catcgtctca
     3961 agcttgatcg acccggcgat gggctgcggc agttcgttcg cgtcgcggcg gcgatacttg
     4021 acggcgaaat agaagatcag caccgtgacg agcacggaga agaagagtgt gatcgccacc
     4081 agataagtgt agagcgcgtc cacctccgtc gagacggtcg aagcctgctc cggcgtaaat
     4141 ggtaatcgtg gcaactgcat ctcgttaagc gattcctcct gcctctcccg tgctcaatcc
     4201 ttccggcggc gatggcgacg cctcctcgcg gcgtcggcga cggcgatgta agagcgagat
     4261 caaaaccacc agcccgatca ccgtcagaat ccccgtcagg cgcatgatgt tcatgatcaa
     4321 cggggcatac ttcccggtcg tcggatcgta gtgatagcaa tagagcgtca acttatcgac
     4381 cacgctcccg atcttccctt ccgacgcttc caccagaccg aacttcaaat cgcgcggcgc
     4441 atactcgatg ccgtagaaat aatgcgacag cttcccttcg ggcgtcgcca gcatcacgcc
     4501 gctcgcgtgc gcgtactggt tcgtcgccgt gtcgaacgag tatttgaaac cgacggcgtc
     4561 ggccagcgcg tcaatcgaag ttttgtcgcc cgtcaggaaa tgccagttgg cggcgacgga
     4621 cgcgtcgccc tgccgcccgt agtcgtgcat gacgacttcc tttttcttga cggcatcgct
     4681 cgcgccctcg ctcggatcga agctcaccgt taccacgtcg aactctttgc ccacggtgaa
     4741 cggcaacgtt ctcaggccgc cgaccagacc gttcagcacg aggttgcaca gcttcgggca
     4801 gttgtaatag acgagcgaga tgaagacggg gcgtttgccg aaatagtcgc cgagccgcac
     4861 gtgctgtccc gtctcgtcgc gaaacttgag ttcgagcgga acctgcccgc cgagacgctg
     4921 ctcgatgccg atctctttca atggcagagg cacgtcgttg ctcgacaccc cgccctgcgg
     4981 cggctgcgcg tactggccga ggacggtgag cggcgagcag caaacggcaa gcagcaggag
     5041 acacgcgacg ccacgcgcaa gccacggcgc acggcttctc tctccgacaa ctcgattacg
     5101 acttatgacc ttgttcatta aactgctcac cgcctactgc tcaatgctta ctgatcgcgt
     5161 ctctccgtct gccgccccgc gctcgcggcg gtcggcaggt ggagtccgtc acgcgacgcc
     5221 gctcctccgc cctgctgttg ctgctgctgt tgctgcggcg gcgactgctg gcgcacgggc
     5281 aagcccttct ccagaagttt cttcttcgcg tcggcaatag ggatgcgcca catccccgtg
     5341 ccggggttaa cttcggcggc agtctccaga tcgtacttga acttcgcctt gagcgcgtcc
     5401 cactccagag tcggctcttt cttctcgaag atcagattcg gatcgtcgag cgagcgatgg
     5461 tcgggcgcgc cttgcaggcg gggcacgccg ggcgggggca ggcgttcctc ggccttgcgc
     5521 gccatcggcg cgggcgcgat ctctttgctc gtcgcgtagg cttcgagcag tttgaacaac
     5581 ccgagcatca aaccgcacac gatgatgagg ccgagaaaca acaacccgac gaacgtgccg
     5641 acgccgcgca cgctcacgtc cgactcttcg tgcatcacat ccgggttcgt gatgtgcgaa
     5701 acgtccggcg tctccgtcag gtgcattttg cctttcccgt tgccgtgctg accaaagatt
     5761 cccatctcaa atcctcgctt ccctaccaac ccgtgaccat taacgcttcg accttatccc
     5821 tcgccccttc gccctttttc cccgacgctt ctcagtgatg gccgctcggt tcggcgagcg
     5881 cgtttgccaa cccttcgtcg ccaagcggaa gcagcgggcg gcttctcagt tcgcgcgtgt
     5941 accaccagag ccagatgccg ccgatcagga tgggcgtcac gaagttcaac gcataagcca
     6001 ccacgtccgg gaagcccccg gcgtgcgatg ccgccccgcc gccgtggtgc ggcatcgcga
     6061 gcgcgctcgg cgcaatcgtc cagaagagat cgacgacgcg catgaagagc acgagcagcg
     6121 cgaccaaaac cagcgcacgc gcgcgccgtt tcagatcgcg cgagagcagg agcacgaacg
     6181 gcagcgcgaa atgaagaatc acgagcgcga ccgcgacata gccccaaccg ccatacaaac
     6241 ggcgcaggta ccagggaatc tcttccggca aattgcccga ccagatgatc aaaaattgcg
     6301 agaacgagaa gtaagcccag agcatcacga aggcgagcag gagtttgccg tgatcgtgaa
     6361 aatggcgcgg ctggtagacg tgcgccaaac gctcgtcctt cgacaaaata acggtggtga
     6421 cgatggcgaa ggccagcgcg gtcacgcccc acccgaccat gatcagcagc ccgtagatcg
     6481 tcgagaacca ctctggttcg agcgacatca cccaatcgac ggcggcgaag gtgacggtca
     6541 aaccgaagag caaaattccg gggccgctca ggttttgcag tttatctttc agacgccgac
     6601 gctcgcgcgg gtctttcgcc gtgtcctgtt cgagcgacca cttgctcagg aaatgctgga
     6661 gcagccacca catcgcaaag tagaagaccg tgcgaccgag aaagaaaggg acgttcaggt
     6721 agatcgcctt gtgcttgatg atctcaaggt cgcgctcctc cggcaccgtc caggggtaga
     6781 ggccgtgcgt gagcacggcg acggcgagcg ggatgaagag gacgaacatc acgggcagcg
     6841 tgcgcgtggc ggcttcgagc gggcgacgaa tcacgacgcc ccaaccgccg cccgacaggt
     6901 ggtgcagcat gatgatggcg agcgaaccga ccgaaacgcc cgtccagaaa acgtagccga
     6961 cgaggtaagc gcgccagaag cccgcgagat tgacgaacgc gccgagcgcg cacaggacga
     7021 gcgcgacgag gccgacgacg agagcgcggc ctccgagtcg ctcgatgttg ggcggggcgt
     7081 tataagttgt agctgtcgtg gcagcaatgt taggcgcgtt catggcgtgc gctcacctcc
     7141 ggctccctga tgtccgccct gtgtgcccgg cgtgcccggc gtgggcttgc gctggttacg
     7201 caagtgctcc ggcacttccg cgacgggcgt gttctggctc agttgcagcg cgcgtatgta
     7261 ggcgatgatc ttccagcggt cttccaccgg aatctgctgt gagtagtcgg gcattgcgcc
     7321 ccagccgttc gtgatcacgt cgaaaaagtg gccgacggtt tcgccgcgca agcggtcgtc
     7381 gtggagggag ggcgggtgac gaaagccgcg ccggacgatc atgccgtcgc cgtagcccgt
     7441 cgcaccgtgg cacatcgcgc agtaaatctc gaaacgctcc tgcccgcgat ccaggtcttt
     7501 ctccgcgatg gggaacggaa actcgttgat gctgcccggc gtcccgctct gccccgccgt
     7561 gccgccgccc gcgccgttcg gaacgttcgc gccgacgttc tggccgcgca cattcacgtc
     7621 gccgccgccc gccgcctgcg cgcccgcgct tccaccctgc gcgttcgcca tcgtcgcctg
     7681 caaagcgttg ccgctaccgt tcatcaaccc ctgcgcgccg cccgtcgtgg ctcccgacgc
     7741 ttgcttcccc gtgtagtaag aggtgttgat gtctcgatag ccgcgcgcga ccgtgccctc
     7801 gacgaaggga cgcgacgact ggccgtcgcc aaaactcttg ctcggctcat aagcctcata
     7861 cctcggctgg tcttgcatgt cctgacgaca accgagagcg agtaagcagc aggccgcgag
     7921 cagcaggcac agggcagcgc gccgaccgaa catcggactc tgacctccgg cctctgacct
     7981 ttgacctctg cgcggttcag tgttcgacat cagccacctc ttgcgcaccc agactgcgta
     8041 gaaagccgct cgtcgcctcg cggtcgaact tcggatcgcg tgattcgatg acgagaaaga
     8101 aacggtcgcg cgtggcgagc gcgaagttcg gcgcgttgaa gaccggatga tacggttcgg
     8161 gcaacccgtt taaggcgagc atgccgaaga tggccgccat cgccgcgccg agcacggtcg
     8221 tctcgaaagt gatcgggatg aaggcaggcc aactgtgcag gggcttgccg ccgacgttga
     8281 tcgggtagtt ccacaccgag acgtaatact gcatggcata gccgccgatc aggccgacga
     8341 tgccgccgat gaggacgatg agcggcagac gctctttatg ggggccgacg gcctccgaca
     8401 gagcctcaat cggataaggc gaataagcgt ccatccggcg atagccttcc tcataagccc
     8461 gccgcgtcgc cgccaccacg tcggtcggat tatcgaactc cgccatcacg ccatagatag
     8521 ctggcctagt cggtttcttc ataagacctc aactacagtg acgagtgacg agcgacgagt
     8581 gacaagataa agaaaaatca aacctgtctt tgaacttgtt cctgctctcg tcatttatca
     8641 ctcaccagtc gtcattgttt tagctcgtca cttgtcactc gtcacttgcc actgctttcg
     8701 gtgctgctcc cggcaggatc gtgcgcatct cgaagatgga gatgacgggc aggaagcgta
     8761 tgaagagcag cagcaacgtg aagaacaggc caatcgtgcc gatgaacatc gaccagtcga
     8821 agatcgtcgg gcgatacatc gcccacgacg acggcagata atcccggtgc agactcgtca
     8881 cgatgatcac gaagcgttcg agccacatgc cgacgctgat gatgagggcg acgatgaaaa
     8941 ggagcaccgt gttgtggcgc gcgcgcctcg accagagaat ttgcgggatg atgatgttcg
     9001 tgatgatgag cagcgcgtaa gcccaccagt agggacccgt ccagatgcgg ttcttgagca
     9061 tgaagccttc atactcgctc gcgctgtacc aggccatgaa cgcttccatc atgtagccgt
     9121 agccgacgat gaggccggtg gcgagcatga ccttcgcgct gttgcgcagg tggcgcagcg
     9181 tgatgaaatc ttcgagtccg tagaacttgc gcaaggggat ggtcaacagt aagaccatcg
     9241 cgaagcccgc atagatcgcg cccgccacga agtagggcgg aaagatcgtg gcgtgccagc
     9301 cgggcagttg cgagacggcg aagtcgaaac tgacgatggt gtgcacggag agcacgagcg
     9361 gcgtagcgag tccggctagc agcaggtagg ccgtctcgta gcgatgccag tggcgcgcgc
     9421 tgccgcgcca gccgagcgcg agcatgccgt agataacttt gaagacgccg ctcttggcgc
     9481 ggtcgcggag cgtggcgaag tcggggatta agccgacgta ccagaagaga gccgagacgc
     9541 tcgcataggt cgagacggcg aacacgtccc acatcagagg gcttctgaat tgcgggtaga
     9601 tgttcatcgt gttcgggtag ggcagcatcc agtaggcgag ccacgggcga cccgtgtgga
     9661 gcagcggaaa cagacccgcg caggcgacgg caaagagcgt catcgcttcg gcgaagcggt
     9721 taatagaggt gcgccactcc tgtcggagca ggaggaggat cgccgagatc agagttccgg
     9781 cgtggccgat gccgatccac cagacgaagt tgatgatgtc gaacgcccac ccgaccggcc
     9841 agttgttgcc ccagatgccg atgccggtcg tgacgagata ggcgatggtg aacatcagga
     9901 cgttcgccag cgagaacgag atgaagaagc cgatatacca gccgagcggc gtcttgcgct
     9961 tgagcacgag cgcgctgatc ttgtccgtca cggacgcggc ggtgtgcccc ggctcgatca
    10021 cggggtttgg cgtgacgtga attaggtcgt cgtgtggctc gtctactctt gatgccatga
    10081 aattttacgc cccatgaatt tcttcgttcg gattgcgcat cgccgacagg tacgtcgtgc
    10141 gcgggcgcgt gttcagttcc gcgagcaaac tatagttgcg tttgtcgtgc tgcatctgcg
    10201 cgacgcggct ctgctggtcg ttcaagtcgc cgaagatgat ggcctcggtc gggcaggccg
    10261 actggcaggc cgtgacgatc tcgccgtcac gcacgcgccg ctcttccttc tccgcttcga
    10321 tgcgcgcgta ggagatgcgc tggatgcagt aagtgcactt ctccatgacg ccgcgcgagc
    10381 gcaccgtgac atccgggttg cgcatcatct tcagggaagg cgtgttgaaa tcctgataga
    10441 gcaggaagtt gaaacggcgc accttgtaag gacagttgtt cgagcaatag cgcgtgccga
    10501 tgcaacggtt gtagaccatg tcgttcgtgc cttccgcgct gtggacggtg gcatgtacgg
    10561 gacagactgg ctcgcacggg gcgacttcgc agtgcataca gggaatcggc atgaagtgtg
    10621 cgccttcggg attttccgcc aagcccttga agtaggtgtc cacgcgcagc cagtgcatgt
    10681 ggcggctgcg cgaaacctga tccttgccga cgatggggat gttgttctcc gattggcacg
    10741 cgacgacgca cgcctgacag ccgacgcacg aagcgatgtc aaccgccatg ccccacttgt
    10801 agcccgtgta ctcgtagttc gggtagagcg tgtcgtttgg tcccggctcc ttgtgcatct
    10861 ctttgccgac gacctcggct gcgccttcgt gcgtcgccac gctcgcgccg ccgtgcacct
    10921 cgcggctgga atcgccgccg cctttcagat attccgtgag cgtgttggag cgcacgatgt
    10981 cgcgcccctc catgttgaag tggagttgcg tgacggcgag caggtactcc tcgcccgtcg
    11041 gcgcgacttc cagccccgcg ccctgccaca tcgcgtcgga cgtgcgcagg cggtaggcgt
    11101 tgaagccgag cggctggtct gcggtgctcg tgccgacttt ccctgcgcgc gtgcgaccgt
    11161 aaccgagatg gactgtcacc acgtcgtccg gctgacccgg cacgatgagg acgggagcgc
    11221 gcaccgtctg cccgcgataa cgcagatcga cgagcggcgc gttgaactca cgccctttga
    11281 aggtgtagtc gttctcgttc agaccgaaca tgcgccgcgc tgtggcgaga ctcatgtagg
    11341 cggcgttgtc ccacgtcaac ttcgtcagag gcttcggcaa ctcctgcaac cagccgttgt
    11401 tggcgaaacg cccgtcgtag atgttcgggt ctggacgaaa gacgatctca aaaccacccg
    11461 ccggagttgg ctgtgccgtc gtcgcgctgg cagaccaatc gcctctcacc gtgacgtttc
    11521 tgactgccgc tgccgtgccc ggcaccactc cgtcgtgcac gaccgcgcgc caggccgcgt
    11581 cgaagtttgc cggagcagca gccgcgccgc gcgtcgcgtt cgtcgccgga ctcggcgttg
    11641 cggtcggcgt cgcgcttggc gtcgggcttg gtatcgcgtt gggcgctgcg cccggtgtgg
    11701 ccgaagcgtt ggggttcgcg ccgtgttcgg cggtcgggat gttctgcgcc gtcgttccgc
    11761 cttgaccgtg cgtgttgtcg ctgcctgcct gcgtggctgc ctgcgacgcg ctcgcggcgg
    11821 cgacgagcga ttcgagacgc tgcgtgcgcc agtagtcgcg cacgatgtcg tagccgctgc
    11881 gttcgggcgc gtcggtaaag gccgctaaga attcgtggac gctgcgcccc ttgtagagcg
    11941 gcgcgatgag cggctggatg atggagactg tcccgtcgtg cgcgcgcgtg tcgctccacg
    12001 cttcgaggaa gtgcgtttcg ggaatgtgcc agtggcagag ttcggctgtc tcgtcgtcgt
    12061 agaggccgag atgtacgcgc aggccgtctt tgccgtcggg cgtcctgatc ttgtcgagcc
    12121 gttccttcgt gaacgcgaga tcgacgggcg cgttatagac cgggttgccg ccgacgatga
    12181 cgagcatctc gacgcgcccc gcttcgatgt cgcccacgag ttcgcgcaga gatgcgactt
    12241 gatcgagggg ctgcgcctca atcgggtcgg tgtggatcac cgtctgcccg acgttgccga
    12301 gcgattgatt catcgcgtgc gcgagcgcgt ggacggcggg cggctggtgt tcgcccgcga
    12361 cgacgattga tctgccgcga tgcgcctgta aatcttttgc gatggcgaaa atgtgcccgg
    12421 cgtgcgcgac gttctgaaac tgcgcgccgt cggtgggcgc acccggcgcg ccgactgcgg
    12481 aggcgagcgc acgcgcgaac atctccatct ggctcggctt gatcgagaaa cgatggtcgg
    12541 cgcacgcgcc ggtgctcgtc ggcgtcgtct ccaccacgta gagacggttc atgtcggtct
    12601 tgcctgccag cacgcgacgc ttcgccgcgt agtcgcgcgc atagcgcagg ttggccgtgc
    12661 cgcaggtgag aaaatcggag tcgagcgaga gcacgcgttc ggcgcggtcg aaacgataga
    12721 tggtgttgac gggctggccg aaagcgagtt gcgcgccaag cgaggcgttg ttcgtcccgg
    12781 cgggttcgta ttgatgccac ttcgcggcgg ggaaggccgc gaggatcgct ctcaactgcg
    12841 cggccatcgt cggcgacgtg acggtttcgg tcaggaaacg cagccccgcg ccctgccgcc
    12901 cgttcatctc accgagtcgc gtgcgaatct cggcgaggaa ggccgtgtaa gggcgtatct
    12961 cgccgcgatg ggtgatggtt tccgaacgat ccgggtcgta gagcgagagg atcgaagcga
    13021 tagagaaaac gtctgtggcg gcgacgcgct ggccgacggg tctttcgggc gtgccgttcg
    13081 tagggtggtc ggggttgcct tcggcgtgcg tcgggcgtcc ttcgttgctg cgcacgagga
    13141 ggccggtcgc gccgccggga aacggcatcg cggtggcgaa gaagaggggc ttgccgggga
    13201 ctaactccgg gggctggtta acgtaaggga cgacggtttc gggcggctgg taagaacagg
    13261 acgtgaggcc ggcgagcgcg agcgacgcgc ccatcaactt caagaacgtg cggcgtccca
    13321 cctcgtcgtc ccactcgccc gcctgtcgcg gaaactccgc gtgcagaaaa tcttcgaact
    13381 gtcccgaatc ggccaactct tcgaggctgc gccagtattc ccgccccgtc ttctcttcga
    13441 ggcgtgagcg tatctcttcg cgcttgaccg ggcggcgaat gttgtaaagt tcggggaaac
    13501 tctctttaat gtgcatctac taacctctcg cgcaacgcct gtcctagcgg tggcaagtcg
    13561 aacaactttc aaggatggac ttcgagcgaa tcttatgctt gacgagcagt tcgcgcccct
    13621 tctgcaactg tgcggcggtc ttgttctccg cgagcgggtt ctcctgctgg tagtccatgt
    13681 tgaagacttc ggatttctcc cgcaggtaac gctccggctg gcggtggcat tccagacacc
    13741 actccatctg caaagaggcc gcctgataga cgagcggcat ctcgtccacg cgcccgtggc
    13801 aggaagagca gccgacgccc ttgttgacgt ggatgctgtg attgaaatag acgtaatcgg
    13861 gcaggtcgtg aacgcgcgtc cattcaatcg gcttgttgtc gcgccaactg gcgcgcacgg
    13921 gttcgagata cgggctgtcg ttccagagtt gcgaatgaca gttcatgcaa gtcttggttg
    13981 acggcagccc cgcgctcgaa gaagtctcga cggacgtgtg gcaataacga caatcaatgc
    14041 cgtcgtcgcc cgtgtggtgc ttgtgcgaaa actgcacggg ctgctcgcgc tcgtcaaacg
    14101 cgcccgtgtt ccacgacgag cggttgatct cggccaccgc ccacgtcaca aacagcagga
    14161 cgaagatcga gccgaagata ctcaacttgg cgagcgtgtt ggtgctgcga tgaaaaattt
    14221 gcggcatgct cagtacctgt tgctttgctg ctcagtgacc ttcaccgacg ggcgaccgtt
    14281 tttgtcaagc atggagaacc gatggtcgct gaaagcgttc gcgtgtcaga gaaagaaagg
    14341 ccaccaccga acgggcgggc gcacaacatg acgaggtacg gcagtcgaga gcttgcaagc
    14401 tacaactttc cccgtcatgc ccccttggtt ccaaaattct tgtggtgggg tagtgatact
    14461 aaattttgcg tcgaacggca agcgattgcc taaatttatt cggaaaataa tctatgaccg
    14521 tcgcgtgacg gtcgcgcccg ccgccgcatc aaacatctct aaccggcgca attgccggga
    14581 aaaatccgct ccagatagcc aagttataag tttaacgcgc gcaaacgctc ataattattc
    14641 atgcctcgcg gctaccgcgc acgccccttc aaactgtagg ataaatcgaa caatttgccc
    14701 gggaggaatt atgaaacaga gaatctcacg cgcttttatt gtcctctcat ttgtgctgct
    14761 ggcggtctgc ggcgcggcgg cgaaggatac ttggacgagt atcaaatcga aaaatttcac
    14821 ggtcgtcggc aacgcgggcg agggcgagat gcgcaaggtg acgatgcgcc tcgaacaatt
    14881 ccgtcaggcc gtctccgtgc tcttcgccaa cgccaaggtc gagacgcgcg tgcccaccac
    14941 cgtcgtcatc ttcaagagcc acggctcgtt caagcccttc aagccgaagt acaagggcaa
    15001 gacgcgggag aacgtcggcg gctacttcat caacggcggc gacctgaact acatcgtgct
    15061 caccacggaa acccacagcg gcatcagccc ctacgagatc atcttccacg aatacgagca
    15121 cttcattctc gccaacagca tgcggcacgc gcccgcctgg ctccacgagg gactcgccga
    15181 attcgtgagc acgcttgaga cttccgacaa cgagcaaaaa gtcaggctcg gcacgcccat
    15241 cgcgcaccac gtctacacac tcagacagaa caatccgatt cctctaaaga ccttcttcac
    15301 ggtcgatcac cgctcgcctc agtacaacga gaacgacaag gtgggaatct tctacgcgca
    15361 gtcgtgggcg ctcatccact acctcatgct cggcaacgac ggcgcgcgtc gcccgcaact
    15421 cacgcgcttc atcaacgggc tgaacggcga cctgcccatc gaagagaatt tccggcaatc
    15481 ctttggcacg gattacgcca agctggagca cgagttgcgc ggctacctga ggaaagaggc
    15541 ttatcccgtg ctggacttca agctgcaaaa gccgctcgaa ctgtcgaagg aggccgtgac
    15601 cacgacgttg accgaggcgc aggtcgaata tcatctgggc gacctgctgc tgcgcgcacg
    15661 tcgtttcgac gaagcaacgg agcgtctgga aaagtcggtc gcgctcgacg ccggtttcgc
    15721 cccgccgcgc gtctcgctcg gcatcgcgcg cctgcgccag aatcgccccg gcgaagcgca
    15781 aaaaatctta cgcggcgcac tcgcgctcga cccgcgcaac ccgctcgctc actatcacta
    15841 cgcctccgcc ctcggcgcgg aagacagact ggaagaggcc gtcgccgctt accgcgaggc
    15901 catcgcgctc aagcctgaca tggcgggcgc gcacgccgaa ctcggacgca cctatcgcaa
    15961 cctgcgtaga aacgaagagg cgttggaggc atacaatcag gccatccggc actcgcccga
    16021 ctcgccctac ttctaccaga gccgcgccta catcaatctc caactcgggc gcggcaacgg
    16081 cgcgtccttc gatgccagag cctacctcca cctccaaggc tggcgcgacg acagcgcgcc
    16141 ctacctcgcg ctcgccgcct acttcggctt ccgacagaag aatcgcgccg ccgatgccgc
    16201 acggatgctc gaagaggccg ccgcgaagac ggacgccgcc gcgtggccgc gccccgtctt
    16261 caaatatctc gcgggcgaac tcacggcgga agaactgatc aagcaggcca acaacaacga
    16321 cgaacttac
//
LOCUS       DASWNS010000023        12147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_234203, whole genome shotgun sequence.
ACCESSION   DASWNS010000023 DASWNS010000000
VERSION     DASWNS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12147
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234203"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..953
                     /locus_tag="VGB76_00620"
     CDS             270..953
                     /locus_tag="VGB76_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEY0097427.1"
                     /translation="MSFLSGLMRDADYQREEPRPAEAFEDGEIVISPDTRREKRLPPG
                     QTRTRKWPVLDAHGTPEIDLANWEFAVEGLVERPQAWSLDEFLALPATKVYADFHCVT
                     RWSRLDNVWGGVATREIAKIAGIKPEAKYVLALAYDYGWTTNIPLEYFLAEDSLVAWS
                     HDGEPIRPEHGGPARLIIPQLYAWKSAKWIKGLRFLAEDRAGFWEEGGYHMRGVPWNG
                     SDGERFRYE"
     gene            1098..1760
                     /locus_tag="VGB76_00625"
     CDS             1098..1760
                     /locus_tag="VGB76_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY0097428.1"
                     /translation="MFLQLPTPAPTPTPPPTLREVFAQLHADLMRALPGMFRGVLVFL
                     VFWVVATAGRRVIALAAPRVRADTGVVLLLSRVYYYGILIFGIVTALGAAGLNVSALV
                     TGLGLTGFALGFALKDVLSNLLSGIMLLLYRPFNIGDLIRMGEYEGTIETIRMRDTLV
                     RAADGRLIVIPNTKLITEVVVNNTRARQNASPRDPDAPRPASAPLAPAPEEDEYWQPP
                     QH"
     gene            1886..3421
                     /locus_tag="VGB76_00630"
     CDS             1886..3421
                     /locus_tag="VGB76_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY0097429.1"
                     /translation="MSNRILNTQPSPAKLNRLARLLCALVVSFIFVGCNSTGSKATEI
                     NNPQVNFAPSTATAATAAAQNSYADVVARVAPAVVTVRSERRGRAAQQHPFMDDPMFR
                     EFFGQRMPNSPQTPRREEGLGSGVIVTGDGYILTNHHVVDGADEIRVEMTDRRVLTAK
                     IVGTDPPSDLAVLKVEATNLPVLPLGDSDKVRVGDIALAVGNPLGIGQTVTSGIISAK
                     ERTTSGVGDGSFANFIQTDAPINRGNSGGALVNTSGELIGINSQILSPTGGSIGIGFA
                     IPSNMAKGVMDQLVKGGKVRRGQLGIQIQEVTSDIAASLDLKEVRGVIIGAVTPGSAG
                     ERAGLKQGDVITSVNGAAVNDVNALRNRIASTAPGTDVTLTVVREGREQQVRATLGEF
                     TQTARSGDEQGDSDSGNGNGAGESTGRLGIRVQPITPELASELQLQSGAQGVVVAEVD
                     PAGPAADAGLQRGDVIVQVNRQPVRTGAGLNEAVGRSGTRPVLLLVNRRGTTVFLTVR
                     PRS"
     gene            3689..5272
                     /locus_tag="VGB76_00635"
     CDS             3689..5272
                     /locus_tag="VGB76_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0097430.1"
                     /translation="MPVNETPAATAAAAGAPRAKADAVPRDAAAVILVREVEGEPQVF
                     WARRSERMAFLGGFYAFPGGQRDAEDAETPVENSADAEEAAMIACAARELFEELGVLV
                     ARGAEHLTRGQLASVLDDLTSARMTFPQLLAHYGLQLDARDFTYAGRWVTPPFSPRRF
                     DTWFFLARCPRKQEPQVRTAEFESGEWTRAKDGVSRWEDCDALVAPPVLHALKVLREG
                     LTEDLVERFLSVPQAQRQPVRRIEFMPGFICFPVRTPTKPPATHTNAYIVGHGELVIL
                     DPGSPDAGEQAELAACIDALLAEGRRVLEIILTHHHPDHVAGVDALRQHLGGRVRVAA
                     HQLTADALRGRVSVDRFIEDGDEIKLAGEPALSLRAMHTPGHTRGHLSFYEERTGALI
                     TGDNILGLGSVLIDPPEGNMIDYLASLERYRALLPKIKVLFGGHGAAVASPRAKIEEY
                     IAHRLEREEHILQAVRAGASSPPEIVRRVYTDVNPKAHALAERAVLAHLEKLEAEKLI
                     AQTGDGNFVAHDANTRAAG"
     gene            5508..6980
                     /locus_tag="VGB76_00640"
     CDS             5508..6980
                     /locus_tag="VGB76_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097431.1"
                     /translation="MAQAKTLGELKRQNYNAGRTVKDELRANLIAKLRAGERIFPGIV
                     GYDETVVPQIVNAILARHNLILLGLRGQAKSRIIRQLTNLLDERIPAIAGSEVNDDPF
                     RPISAYGRQLIEELGDETPVAWIERDARFVEKLATPDVTIADIIGDVDPIKAAKGGHL
                     LSDELTIHYGLLPRANRGIFAINELPDLAGKIQVGLFNIMQEGDVQIKGYPVRLRLDV
                     MLVFSANPEDYTARGKIITPLKDRIGAEVSTHYPADLQLGVEITKQEAWTKREDFAGR
                     LRVPDFIRELVEQIAFEARDDQRIDKRSGVSQRLPITVMESLVSNAERRALLTGEEEI
                     VPRISDIYAAVPSMTGKMELEYEGEQVGATKIAKDLIKRAAGEIHEGYFVGIDFTAAV
                     RWFDEGNNLRLADTASASECVALLEAVPELIDTVVIPFDFKRTDEAQLIAACEFALEG
                     LYAQNKISRNEEGGYTAATKAKKDRRGMIYDDLTETGRFS"
     gene            6994..7209
                     /locus_tag="VGB76_00645"
     CDS             6994..7209
                     /locus_tag="VGB76_00645"
                     /inference="COORDINATES: protein motif:HMM:NF044561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY0097432.1"
                     /translation="MDRTYNAVYLKVPEGYVGFIEELPGVSTQGATLEEARQNLKEAV
                     TIVLEANQALTLVATDGEEVIREPLSI"
     gene            7240..7455
                     /locus_tag="VGB76_00650"
     CDS             7240..7455
                     /locus_tag="VGB76_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097433.1"
                     /translation="MSKSNHEEQRTESPEEAHGRARELARRQGVEPIRGIEDLRGDFW
                     PEEDNADEFLSWVRTLRQDDARRSIPE"
     gene            7452..7862
                     /locus_tag="VGB76_00655"
     CDS             7452..7862
                     /locus_tag="VGB76_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0097434.1"
                     /translation="MTTTGAIVVVDTDVVSFIFKGDTRGSLYRPHLDGRLAIIAAQTR
                     AELELWTLLRKWGERRRTAMRTYLKNFVFAEADETICLRWAEVQENAQRRGRPISCAD
                     GWIAATALAYAVPLVTHNPDDFKNVSGLTILTEK"
     gene            7870..9228
                     /locus_tag="VGB76_00660"
     CDS             7870..9228
                     /locus_tag="VGB76_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0097435.1"
                     /translation="MKFIRYSKFKGFDVSGVNLGDLMEALQDSLLQSGFDDDYYWTRQ
                     RRPQDTSLDALRQALLRALLESGEVDEQQVREMLAENEGQFKGSQLEELLNQLIERLV
                     EEGFLNLREEPSPEQQRRMRGQAGMGRADETPPPRNVKFEVTEKGLDFLGYKTLRNLL
                     GSMGKSSVGRHDTPHLATGIEAAQASKLYEFGDTVNLDVNATLMSAIKREGLGVPLNL
                     EYRDLYVHQCDYQSSCATVVMLDCSHSMILYGEDRFTPAKKVALALAHLIRTQYAGDS
                     LKVVLFHDDAEELPLARLATTQVGPYHTNTAEGLKLARRLLQAQRKEMKQIVMVTDGK
                     PTACFLEGNGLAARRNSSGGEMRRSSVGSSSGGGARRLYKNSMGLDPIVMDATFNEVQ
                     ACRKAGIMINTFMLASDYYLVEFVKQMSQMTNGKAYFATPGNLSQFVLIDFLKRKKSR
                     VK"
     gene            complement(9316..9669)
                     /locus_tag="VGB76_00665"
     CDS             complement(9316..9669)
                     /locus_tag="VGB76_00665"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY0097436.1"
                     /translation="MADEVSNTCDLASRKCVPCHGGVPRLVGAEIEQFMGELEGWEVV
                     AEHHLKKTYAFPNFRQALALVNRVGEVAEAEGHHPDICFGWGRVELTIYTHAIDGLSE
                     SDFILAAKIDAIQKG"
     gene            complement(9832..10464)
                     /locus_tag="VGB76_00670"
     CDS             complement(9832..10464)
                     /locus_tag="VGB76_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEY0097437.1"
                     /translation="MRQKICGVEKVCLLKVCLLKGCLLIVSLALCVVISATGARGQQA
                     QQPSVYAGEERREIKSLSREEIEQLLQGHGMGLAKAAELNHYPGPRHVLELGERLKLT
                     PEQRTATEGAFARMREEAMRLGRQIVARERELDAMFAKGEIDAGKLRASTAEIARLQG
                     NLRAAHLAAHLETRRILSPQQIAKYDELRGYAGAGKQTPPSPHEHKHGKH"
     gene            complement(10570..>12147)
                     /locus_tag="VGB76_00675"
     CDS             complement(10570..>12147)
                     /locus_tag="VGB76_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097438.1"
                     /translation="YQSWPGRRIYVNADAGLIEIEDQKMWSPAVFGVGQLGPLADLSV
                     YNLTLSLIGCPVRAYGCASGRLSGRIAAFFYRYKSIGGFEYVADFLIGSRGDRPLLTR
                     PGDSGTVWVVETDDVRRDLMPIAVQWGGAVFSADTTRLPFALATNLSTICRELEVDLF
                     RSRSLASFEYWESVGHYSIASFACAQVGDKSLRDLMILNRRRISFDPDAIDTSVNDVT
                     VPGFVPLANVPDKVWKKSFDEKKAPYGRKGNENPNHYADIDYPDADGNTLDDLTPTVA
                     FLDPQTWRNYYKSIGWNSVSQRGLLPFRVRQIYKKMVEFVQAKDVVQYVAAAGILSHY
                     VGDACQPLHGSYMSDGDPFRHRDGTPSDVELGLGSGYGGGIHGAYEGELLDLHVDDFL
                     SGLTATLGVSHGMALVGNRFEAGFAIIELLRRARGHINPQTLVHRYGDLILNGVKKSK
                     RIEILWDEFGAQTIEVMADGARTLAMLWESAWVEGGGDEIPQSELKRFDRSRLQRIYE
                     RLDFLPSFALGQIDKHI"
BASE COUNT         2444 a         3714 c         3699 g         2290 t
ORIGIN      
        1 gctacgagag cgagcgcggc cacagcggag ccgcagacga atggcacgac cgcagcgtcg
       61 gcatcaaaga gccgctcgcc aaaggcgtca acaagtggat gcccgaagag ttgatgaacc
      121 tcgcggagcg gtcaacgaag aagtgacagg tgacgagaaa gaaaagtaat gagtgatgag
      181 taatgagtga tgagttaaga caaaagcctt atctttgact catcactcat cactcatcac
      241 ttcttcctcg tcactggttt gaggttgcta tgagttttct gagcggcttg atgagggatg
      301 cggattacca gcgcgaagag ccgcgcccgg cggaggcgtt cgaggacggc gagatcgtca
      361 tcagccccga cacgcggcgc gagaagcgtc tgccgcccgg ccagacgcgc acacgtaagt
      421 ggcctgtgct cgacgcgcac ggcacgccgg agattgatct ggcgaattgg gagtttgccg
      481 tcgaaggtct ggtggagagg ccgcaggcgt ggtcgctcga cgaattcctc gccttgcccg
      541 caaccaaagt ttatgcggac tttcattgcg tgacgcgctg gtcgcgcctc gacaacgtgt
      601 ggggcggcgt ggcgacgcgc gagatcgcaa agatcgccgg gatcaaaccg gaagcgaaat
      661 acgtgctcgc gctcgcttac gattacggct ggacgacgaa catcccgctc gaatatttcc
      721 tcgccgaaga ttcgctcgtc gcctggtcgc acgacggcga gccgatccgt cctgaacacg
      781 gcggccccgc gcgtctcatc atcccgcaac tctacgcctg gaagtctgcc aagtggatca
      841 agggcttgcg ctttctcgcc gaagaccgcg cgggcttctg ggaggagggc ggctaccaca
      901 tgcgcggcgt cccctggaac gggagcgacg gcgaacgctt ccgctacgaa taaggcagtg
      961 acgggtgaca agttaaaaag acgtaaattg tgaaccgtaa attttgaata gagaaaacct
     1021 gctttcttct attcacgatt cacgattcat ttgtctttca ctcgtcactt gtcactcgtc
     1081 acttgtcact gtcttctatg tttcttcaac tgccaacgcc cgccccgacg ccgacgcctc
     1141 cgccgacgct cagggaagtt ttcgcgcagt tacacgccga tctgatgcgc gctctgcccg
     1201 gcatgttccg gggcgtgctg gtcttcctcg tcttctgggt cgtggcgacg gcggggcggc
     1261 gtgtgatcgc tctggccgcg ccgcgcgtgc gtgccgacac gggcgtcgtg ttgctgctct
     1321 ccagagtcta ttattacggc attctcatct tcggcatcgt caccgcgctc ggcgcggcgg
     1381 gcttgaacgt gagcgcgctc gtgacgggtc tgggcttgac gggctttgcg ctcggcttcg
     1441 ccttgaaaga cgtgctctcg aacctgttgt cgggcatcat gctgctgctc tatcgcccct
     1501 tcaatatcgg cgatctcatc aggatgggcg agtatgaggg gacgattgag acgatccgca
     1561 tgcgcgatac gctcgtgcgc gccgccgacg ggcggctcat cgtcatcccc aacaccaaac
     1621 tgatcacgga agtcgtcgtc aacaacacgc gcgcccgtca aaacgcctca ccacgcgacc
     1681 cggacgcccc ccgccccgcc tccgcgccgc tcgcacccgc cccggaagaa gacgaatact
     1741 ggcaacctcc gcaacactaa ttttacattc ttaacgcaac cgcgcaccac ctggatcgta
     1801 tctaaatacc aaagatacga ggtagttgat ctgatcgaga acaactggga aaatagagct
     1861 atttttatca acagaggtgc agcatatgag taacagaatt cttaacacac aaccgagtcc
     1921 ggcgaagttg aaccggctgg cgcggctgct ttgtgcgctg gtcgtgtcgt ttatcttcgt
     1981 ggggtgcaat tcgacgggtt ctaaggcgac ggagatcaat aacccgcagg ttaacttcgc
     2041 gccgagcacg gcgaccgccg ccaccgccgc cgcacagaac tcctacgccg atgtggtggc
     2101 gcgcgtcgcc ccggcggtcg tcacggtgcg ctccgagcgg cgcgggcgcg cggcgcagca
     2161 gcacccgttc atggacgacc cgatgttccg cgagttcttc gggcagcgca tgccaaactc
     2221 gccgcagacg ccgcgccgcg aagagggttt aggctccggc gtcatcgtca cgggcgacgg
     2281 ctacattttg acgaaccatc acgtcgttga cggcgcggac gagattcggg ttgagatgac
     2341 cgaccgccgc gtgctgacgg cgaaaattgt cggcaccgat ccgccgtccg acctcgcggt
     2401 cttgaaggtc gaggcgacga acctgccggt cttgcccctc ggcgactcgg acaaggtgcg
     2461 tgtcggcgac atcgcgctcg ccgtgggcaa cccgctcggc atcggccaga ccgtgacctc
     2521 cggcatcatc tccgccaagg agcgcacgac ctccggtgtc ggcgacggca gcttcgccaa
     2581 cttcatccag accgacgcgc cgatcaatcg cggcaactcc ggcggcgcgc tcgtcaacac
     2641 gagcggcgaa ctgatcggca tcaactcgca gattctctcg cccacgggcg gcagcatcgg
     2701 catcggcttt gccatcccgt cgaacatggc gaagggcgtg atggatcaac tcgtcaaggg
     2761 cggcaaagtg cgtcgcggcc aactcggcat ccagattcag gaggtcactt cggacatcgc
     2821 tgcgagcctc gatctgaaag aagtgcgcgg cgtcattatc ggcgcggtca cgccgggcag
     2881 cgcgggcgag cgcgccgggc tgaaacaggg cgacgtgatc acgtccgtca acggcgcggc
     2941 ggtcaacgat gtcaacgccc tgcgcaatcg tatcgcgagc accgcgccgg gcacggacgt
     3001 gacgctcacg gtcgtgcgtg aaggccgcga acagcaggtg cgcgccacgc tcggagagtt
     3061 cacgcaaacc gcacggagcg gcgacgaaca gggcgactcg gattcgggta acgggaacgg
     3121 cgcgggcgag agcaccggac gactcggcat ccgcgtgcag ccgatcacgc ccgaactcgc
     3181 cagcgagttg caactgcaat cgggcgcaca gggcgtggtc gtggccgaag ttgacccggc
     3241 gggtcccgcc gccgacgccg gactccagcg cggcgacgtg atcgtgcagg tcaaccgtca
     3301 acccgtgcgc acgggcgcag gcttgaacga ggccgtcggg cgcagcggca cgcgccccgt
     3361 cctcctgctc gtcaatcgtc gcggcacgac cgtgtttctc acggtgaggc cgcgctcgta
     3421 aagcagcagg cggcgtttaa gaagttagta aaaacttaga aggtgaaggg gacaagcgcg
     3481 tcttgcgccg tccgcttcac cttctggttt tatggcggcg atagattgcc gggcgcgcga
     3541 aagcaatcac tatagtttta ccttttcgtg tcagacaggc tttagccgga cgttgatgtt
     3601 atactgctct caactcccgt aaacgattga gcctgaattt tcggagcacg cttctcgtct
     3661 ctcacgaaga acgagttttc atttgaagat gcctgtcaac gaaacaccag ccgccacggc
     3721 agcagcagcg ggtgcgcccc gcgctaaagc cgacgccgtg cccagagatg ccgccgccgt
     3781 catcctcgtg cgcgaggttg agggcgagcc gcaggttttc tgggcgcggc gcagcgaacg
     3841 catggccttt ctcggcggct tctacgcctt ccctggcggg cagcgcgatg cggaagatgc
     3901 ggaaactccg gtcgaaaact cggctgacgc ggaagaggct gcgatgatcg cgtgcgcggc
     3961 gcgcgagttg ttcgaagaac tcggcgtgct cgtcgcgcgt ggcgcggaac atctgacgcg
     4021 aggccaactc gcctccgtcc tcgacgatct cacgtccgcc cgcatgacct tcccgcaact
     4081 gctcgcgcac tacggcctgc aactcgacgc gcgcgatttc acctacgccg ggcgttgggt
     4141 gacgccgcct ttcagcccgc gccgcttcga cacctggttc tttctcgcgc gctgcccgcg
     4201 caagcaggag cctcaggtgc gcacggcgga gtttgagtcg ggcgaatgga cgcgtgcgaa
     4261 ggatggcgtc agccgttggg aggattgcga tgcgctcgtc gcgccgcccg tgctgcacgc
     4321 gctgaaagtt ttgcgcgaag ggttgacgga agatttggtc gaacgttttt tgagcgtgcc
     4381 gcaggcgcag cgtcaacctg tgcgccgcat cgagttcatg cccggcttca tctgctttcc
     4441 cgtgcgcacg ccgacgaagc cgcccgccac gcacacgaac gcctacatcg tcggtcacgg
     4501 cgaactcgtc atactcgatc ccggctcgcc cgacgcgggc gaacaagcgg aactcgccgc
     4561 ctgcatagac gcgcttctgg cggaaggccg acgcgtgctt gagatcatcc tgacgcatca
     4621 tcacccggac cacgtcgcgg gcgtggacgc gcttcgacag catctcggcg ggcgcgtgcg
     4681 ggtcgccgcg catcaactga ctgccgatgc cttacgcggg cgcgtctccg tggaccgctt
     4741 catcgaagac ggagacgaga tcaaactcgc gggtgagcct gcgctctccc tgcgcgcgat
     4801 gcacacgccg ggacacacgc gcggccatct cagcttttac gaagaacgga cgggcgcgct
     4861 catcacgggc gacaacattc tcggcctcgg ctcggttctc atcgatccgc ccgaaggcaa
     4921 catgattgac tatctcgcgt cgctcgaacg ctaccgcgcg ctcttaccga agatcaaagt
     4981 tctcttcggc gggcacggcg cggcggtcgc cagcccacgc gcgaagatcg aggaatacat
     5041 cgcgcacagg ctcgaacgcg aggagcatat tctgcaagcc gtgcgtgcgg gcgcatcttc
     5101 gccgccggag atcgtccggc gcgtctacac ggacgtaaac ccgaaggctc acgcgctggc
     5161 cgagcgcgcc gtcctcgcgc atctcgaaaa gctcgaagcg gagaagttga tcgcgcagac
     5221 aggcgacggc aactttgtcg ctcacgatgc gaacacgcgg gcggcgggtt gagccgtttc
     5281 gcaaaactcg aagtttaatc gtctgcaaaa ccgtgtacgg agggcgatga atttctggca
     5341 gggaaagaga gtgctggcat ctcccttgag aatgtgaaaa tgaggcgttg agcatcaggc
     5401 gcgcgtgcct ctccgtttct cattttccgt ttctcgacga gaacggtttc acagaggcat
     5461 ctgcgcaggc agcaattatt caaacgacaa ggaacgaatt tcaaacgatg gcacaggcaa
     5521 agactttagg cgagttgaaa cgacagaact acaacgccgg gcgcacggtc aaggatgaac
     5581 tgcgcgcgaa cctcatcgct aaactgcgcg cgggcgagcg catcttcccc ggcatcgtcg
     5641 gctacgacga gacggtcgtg ccgcagatcg tgaacgccat cctcgcgcgc cacaacctga
     5701 tcctgctcgg acttcgcggt caggccaaga gccgcatcat ccgccagttg acgaacctgc
     5761 tcgacgagcg tattcccgcc atcgccggtt cggaagtcaa cgacgatccg tttcgcccga
     5821 tcagcgcgta tggtcgccag ttgatcgaag agttgggcga cgagacgccg gttgcgtgga
     5881 tcgaacgcga cgcgcgcttc gtcgaaaaac tcgccacgcc cgacgtgacc atcgcagaca
     5941 tcatcggcga cgttgatccg atcaaagccg cgaagggcgg ccatctctta agcgacgaac
     6001 tcacgattca ttacggcctc ctgccccgcg ccaatcgcgg catcttcgcc atcaacgaac
     6061 tgcccgacct cgccggaaaa attcaggtcg gcctcttcaa catcatgcag gagggcgacg
     6121 tgcagattaa aggctacccc gtgcgcctgc ggttggacgt gatgctcgtc ttctccgcta
     6181 accccgaaga ctacacggcg cgcggcaaga tcatcacgcc cttgaaagac cgcatcggcg
     6241 cggaagtctc cacgcactac ccggcagacc tccaactcgg cgtcgagatc acgaagcagg
     6301 aagcctggac gaagcgcgaa gacttcgccg gacgtctgcg cgtgcccgac ttcatacgtg
     6361 aactcgtcga gcagatcgcc tttgaagcgc gcgacgacca gcgcatagac aaacgctccg
     6421 gcgtctcgca aagactcccg atcaccgtca tggaatcgct cgtctcgaac gccgaacggc
     6481 gcgctctcct gacgggcgag gaagagatcg tcccgcgcat ctccgacatc tatgccgccg
     6541 tgccctcgat gaccggcaag atggaactcg aatacgaagg cgagcaggtc ggcgcaacga
     6601 agatcgccaa agacctgatc aagcgcgccg cgggggagat tcacgaaggc tatttcgtcg
     6661 gcatagactt cactgctgcc gtccgctggt tcgacgaagg caacaatttg cgcctcgccg
     6721 acacggcctc ggcctccgag tgcgtcgcgc tcctcgaagc cgtgcccgaa ctcatcgaca
     6781 ccgtcgtcat tcccttcgac ttcaaacgca ccgacgaagc tcaactcatc gccgcctgcg
     6841 agtttgccct cgaaggtctg tacgcgcaaa acaagatcag ccgcaacgaa gaaggcggct
     6901 acaccgccgc gacgaaagcc aagaaagatc gacgcgggat gatctatgat gacctgacgg
     6961 agacagggcg tttcagctaa ggagccaacc gcaatggatc gcacctacaa cgccgtctac
     7021 ctcaaagtgc ccgaaggcta cgtcggcttc atcgaagagt tgcccggcgt gagcacacaa
     7081 ggcgcaacgc tggaagaagc gcgtcagaac ttgaaagagg ctgtaacgat tgtgctcgaa
     7141 gctaatcaag ccttaacgtt ggtagcgact gacggtgaag aggtcatccg cgaaccgctt
     7201 tcaatataaa acaggttggc gatagggagg cgatgcccga tgagcaagtc gaatcatgaa
     7261 gagcagcgga cggaatctcc ggaggaagcg cacgggcggg cgcgtgagct tgcccggcgt
     7321 cagggcgtcg agccgattcg cggcatagaa gatttgcgtg gagacttctg gccggaagag
     7381 gataacgcag acgagttctt gtcgtgggtg cgtactcttc gtcaagatga tgctcgccgg
     7441 agcattcctg aatgacgact accggggcga tcgtagtggt tgacacagac gttgtttcgt
     7501 tcatcttcaa aggcgacacg cgcggcagtc tgtaccggcc tcacctggac gggcgactcg
     7561 caattatcgc agcgcagacg cgagctgaac tcgaactctg gacgctgctg cgaaagtggg
     7621 gcgagcgtcg ccgcactgcc atgcggacat atctaaagaa tttcgttttc gcggaagcgg
     7681 atgaaactat ttgtctgcgc tgggctgaag tgcaggagaa tgcacaacgc agggggcgcc
     7741 ctatttcctg cgccgacggg tggatcgccg ctactgcgct ggcctatgcc gtgccgttgg
     7801 tcacacataa tcctgacgat tttaagaatg tatcagggtt gacaatactt acagagaagt
     7861 agagcagtta tgaagttcat acgttattca aaattcaaag gcttcgatgt gtcgggcgtc
     7921 aatctgggcg acttgatgga ggcgttacag gatagtttgc tgcaatcggg ttttgacgac
     7981 gattactatt ggacgcggca gcgcaggccg caggacacgt ctttggatgc gttgcggcag
     8041 gcgttgttgc gtgcgttgtt ggagtcgggt gaggttgacg agcagcaggt gcgggagatg
     8101 ctggcggaga atgagggaca gttcaagggg tcgcaactgg aagagttgtt gaatcaattg
     8161 atcgaacgtc tggtggaaga gggctttttg aatctgcgcg aagagccgtc gccggaacag
     8221 cagcgacgca tgcgcgggca ggcaggcatg ggacgcgccg acgaaacgcc gccgccgcgc
     8281 aacgtgaagt ttgaagtgac ggagaagggc ttggattttc tcggctacaa gacgctgcga
     8341 aatctgctcg gcagcatggg taagagttcc gtggggcggc acgacacgcc gcacctggcg
     8401 acgggcatcg aagcggcgca ggcgtcgaag ttgtacgagt tcggcgacac ggtgaatctg
     8461 gacgtgaacg cgactttgat gtcggcgatc aagcgcgagg gcttgggcgt cccgctcaat
     8521 ctcgaatacc gcgacctcta cgtacaccag tgtgattatc aatcttcgtg cgcgaccgtg
     8581 gtgatgctcg actgctcgca ctcgatgatt ctctacggcg aagatcgttt cacgcccgcc
     8641 aaaaaggttg cgctcgcgct cgcgcatctg atccgcacgc agtatgcggg cgactcgctc
     8701 aaggtggtgc tctttcacga cgacgcggag gagttgccgc tcgcgcggct ggcgaccacg
     8761 caggttggtc cttatcacac gaacactgcc gagggtctga aactcgcgcg gcgtctcttg
     8821 caggcgcagc gcaaagagat gaagcagatc gtgatggtga cggatggcaa gccgaccgcc
     8881 tgttttctgg aaggcaacgg cctcgcggcg agaagaaact ccagcggcgg cgagatgcgc
     8941 cgttcaagcg tcggcagtag tagcggcggc ggcgcgcggc ggctctacaa gaattcgatg
     9001 gggcttgacc cgattgtgat ggacgccact ttcaacgaag tgcaggcgtg ccgcaaggcc
     9061 ggaatcatga tcaacacgtt catgctggcg tcggactact acctcgtcga gttcgtcaag
     9121 cagatgtcgc agatgaccaa cggcaaagcc tacttcgcca cccccggcaa cctctcacaa
     9181 tttgtcctga tagattttct caaacgcaag aagagccggg tgaaatgagg gatgaaggat
     9241 gaaggatgag ggatgaatca gaaaagcatc caacgaagcg acagtctttc ttattcatcc
     9301 ctcatccctc atccttcatc ccttttgtat cgcgtctatc ttggcggcga ggatgaagtc
     9361 gctttcgctg aggccatcga tggcatgcgt gtagatggtc agttcgacgc gcccccagcc
     9421 gaagcagatg tccgggtgat gcccctcggc ttccgccact tcgccgaccc gattgacgag
     9481 cgcgagcgcc tgccggaagt tggggaacgc gtaggtcttc ttcaggtgat gctctgcgac
     9541 gacttcccag ccctccaact cgcccatgaa ctgttcgatc tccgcgccca cgagacgcgg
     9601 caccccgcca tgacatggca cgcacttacg gctcgccaga tcgcacgtgt ttgaaacttc
     9661 atccgccatt ttaaattgtt ccttcgcgct aatctcaaga ttacgaaagg ctcgacgctt
     9721 taagttttct tcgcctcctg cttgcgcctc tttcgtgaaa ccattctctc gccggaggcg
     9781 cacggaggca aacgaagacg gcgcgtggtt ctgtcacccg cgcccggtct tctagtgttt
     9841 gccgtgtttg tgttcgtgcg gcgaaggggg cgtctgcttg cctgccccgg cgtagccgcg
     9901 cagttcgtca tattttgcga tctgctgcgg cgagagtatg cggcgcgtct caaggtgcgc
     9961 ggcgaggtgt gcggcgcgca agttaccttg cagccgcgcg atctccgccg tggacgcgcg
    10021 gagcttcccc gcatcaattt cgcctttcgc aaacatcgca tcaagctcgc gctcgcgcgc
    10081 cacgatctgc cgaccgagcc tcatcgcctc ttcacgcatc cgtgcaaacg cgccctcggt
    10141 cgccgtgcgt tgctccggcg tcaactttag ccgttcgccc agttcgagca cgtggcgggg
    10201 tccgggataa tgatttagct ccgccgcctt cgccagtccc attccgtgac cctgcaaaag
    10261 ctgctcgatc tcttcccgcg agagcgactt gatctcgcgt cgctcctcgc ccgcatagac
    10321 gctcggctgc tgcgcttgct gcccacgcgc tcccgtcgcc gagatgacca cgcagagcgc
    10381 gagcgacaca atcaacaaac agcctttcaa cagacagacc ttcaacagac agactttttc
    10441 caccccacaa attttttgac gcatcagttc atcctccctg atgcacgatt gtaagccctg
    10501 tattcgacta cagggtcaag ccctttcttc tgcgggtcgt ttatggtttt cgcctgtgcc
    10561 agccgccatt cagatatgtt tatcgatctg gccgagcgcg aatgacggca ggaagtcgag
    10621 ccgctcgtaa atcctctgaa gtctgctcct gtcgaacctc ttcagttcag actgcggaat
    10681 ctcgtcgccc ccgccttcca cccacgcgct ctcccagagc atcgccaaag tgcgtgcgcc
    10741 gtcagccatc acctcaatcg tctgcgcgcc gaactcatcc cagagaattt caatgcgctt
    10801 gctcttcttc acgccgttga ggatgaggtc gccgtacctg tgaacgagcg tctgcgggtt
    10861 gatgtggccg cgcgcgcgcc ggagcagttc tatgatggcg aagccagcct cgaacctgtt
    10921 cccgacgagc gccatcccgt gagaaacgcc gagagtcgcg gtcaagccgg agaggaagtc
    10981 gtcaacgtgc aggtcgagca gttcgccctc gtaagcgccg tggatgccgc ccccgtaacc
    11041 actgccgagg ccgagttcga catcggacgg cgttccgtca cggtgacgga agggatcgcc
    11101 gtcggacatg taggagccgt gcaaaggctg gcacgcatcg ccgacgtaat gcgacaggat
    11161 gccagcggcg gcaacgtact gcactacgtc tttagcctga acgaactcga ccatcttctt
    11221 gtaaatctgc cggacgcgga acggcagcag gccgcgctgc gagacggagt tccagccgat
    11281 ggacttgtag tagttgcgcc aggtttgcgg gtcgaggaat gcgaccgtcg gcgtgagatc
    11341 gtcgagcgtg ttgccgtccg catccgggta gtctatgtcg gcgtagtgat tcgggttctc
    11401 attgcccttg cgcccgtaag gcgctttctt ctcgtcaaac gatttcttcc agactttgtc
    11461 gggcacgttg gcgagcggga cgaagcccgg cacggtgaca tcgttgacgc tcgtatcaat
    11521 cgcatcgggg tcgaagctga tgcggcggcg gttcaatatc atcaagtctc gcaggctctt
    11581 gtcgcccacc tgtgcgcagg cgaacgaggc tatcgagtaa tggccgaccg actcccagta
    11641 ttcgaaagag gccagcgagc gcgagcggaa cagatcgact tcgagttcgc ggcagatggt
    11701 gctgaggttc gtggcgagcg cgaaggggag ccgggtggtg tccgccgaga agaccgcgcc
    11761 gccccactgc acggcaatgg gcatcaggtc gcggcgcacg tcgtcggttt cgacgaccca
    11821 caccgtaccc gaatcgccgg ggcgcgtcag cagcggcctg tcgccccttg agccgatgag
    11881 aaagtcggcc acgtactcga agccgccgat tgacttgtag cggtagaaga aggccgcgat
    11941 gcgccccgac agccgaccgc tggcacagcc ataggcgcgc accgggcagc cgatcaggct
    12001 caacgtcagg ttgtagacgc tcaggtcggc gagtggtcca agctgcccga cgccgaaaac
    12061 tgcgggactc cacatcttct ggtcttcgat ctcgatcagt cctgcgtcag cgttcacgta
    12121 gatgcggcga cccggccagc tttgata
//
LOCUS       DASWNS010000024         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_699, whole genome shotgun sequence.
ACCESSION   DASWNS010000024 DASWNS010000000
VERSION     DASWNS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..386)
                     /locus_tag="VGB76_00680"
     CDS             complement(183..386)
                     /locus_tag="VGB76_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097439.1"
                     /translation="MRDKQSSPQKLSDETRAIEQAMRRAVGHALLAHKRAGNTVAAWK
                     DGEVVLVRAENIQLEGLVQGKQK"
     gene            complement(383..988)
                     /locus_tag="VGB76_00685"
     CDS             complement(383..988)
                     /locus_tag="VGB76_00685"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEY0097440.1"
                     /translation="MPDSLPTRSSPPQVFIIAGATGAGKSTLAPFLLRDAFGLNEFVN
                     ADTIALGLSAFSPESVTFEAGRIMLARLRDLARTRQSFAFETTLATRSYAAFVAGLRR
                     EGYEFQLLYLYLRSPELAVERVRSRVRLGGHNVPAPVVHRRFHNGARNFFRLYRPLAD
                     TWGMYDNSTLDAPRPVAVGHRDEFTRVLQQDLWQDFQRRAT"
     gene            complement(995..1891)
                     /locus_tag="VGB76_00690"
     CDS             complement(995..1891)
                     /locus_tag="VGB76_00690"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEY0097441.1"
                     /translation="MPDEQSTRDRVRALVREVLNNALSEEESGERADASQTASPSYAS
                     APAPPPAAAPAPPKTTPAAAPPRATTFAPPPAGAKSATSAPEDKPIARDESAKTVITE
                     ADVHGLERGARLRIAEGARLTPLAADIVRERGIELVRRPSRRGSRESKSIAVGADHGG
                     YPLKEELKTYLAELGQRVRDFGTNSTDAVDYPDFAHAVARAVADGECDLGIIIDGAGV
                     GSCMTANKVPGVRAAACYSVRVAVNSREHNDANVLTLGSGTITPAEMREIVSAWLSTG
                     ITEERHRKRVAKIEAVERQYRA"
     gene            2107..2676
                     /gene="hpt"
                     /locus_tag="VGB76_00695"
     CDS             2107..2676
                     /gene="hpt"
                     /locus_tag="VGB76_00695"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY0097442.1"
                     /translation="MSASSALIPSEFTNDNLEPLFSAEEIQNRITSLGAEIARDYAGR
                     NPLLLGVLKGAVIFLSDMMRAIDLRLGVEFMAISSYGEATRSSGEVKIVKDLDVPIEG
                     RDILIVEDIVDTGLTLSYLMANLRSRGAASVKLVALLNKQERRIREVQIDYLGFTIPD
                     AFVVGYGLDFAERYRNLPYIAVIKDPSKQ"
BASE COUNT          489 a          809 c          867 g          541 t
ORIGIN      
        1 gtcaaccacg cgctgcatcc gctccgggca acgattgaaa caggccgtcc cgcacccgca
       61 caacgccgcc tgctcctccg catccccatt cagacgcgcc acaatcacat ccgtgatctg
      121 ctcaacaagg tgctccaaat cgccgttacc ggaatatgac atcgactcta atgctcctga
      181 aattatttct gtttgccttg tactaaccct tccaactgaa tgttttcagc acgaactaaa
      241 acgacctccc cgtctttcca ggcggcgacg gtgttgcccg cgcgtttgtg tgcgaggagg
      301 gcgtgtccga cggcgcgacg catggcctgt tcgatggctc tggtttcgtc ggatagtttc
      361 tgcgggctgc tctgtttgtc tctcatgttg cgcgtctctg gaaatcctgc cacaaatctt
      421 gttggagcac tctggtaaat tcgtctcgat ggccgacggc gacggggcgc ggcgcgtcga
      481 gggtggaatt atcgtacatt ccccacgtat cggctagcgg tcggtaaagc cggaagaagt
      541 tgcgcgcgcc gttgtggaaa cgcctgtgga cgaccggcgc ggggacgttg tggccgccga
      601 gtcgcacgcg cgagcggacg cgttcgacgg cgagttccgg gctgcgcagg tacaggtaga
      661 gcaattgaaa ttcgtaaccc tcgcgccgca agcccgcgac gaaggcggca taggagcgtg
      721 tggcgagcgt cgtctcgaaa gcgaagctct ggcgtgtgcg tgcgaggtcg cgcaggcgtg
      781 cgagcatgat gcgccccgcc tcgaaggtca cgctttcagg actgaacgca gagaggccga
      841 gcgcgatggt atcggcgttg acaaactcgt tgaggccgaa cgcatcgcgc agcagaaacg
      901 gcgcgagcgt ggatttcccc gccccggtcg ccccggcgat gatgaagact tgcggcggcg
      961 atgatctcgt cggcagcgaa tcaggcatcg aaactcacgc gcgatattga cgctcgaccg
     1021 cctcgatctt cgccacgcgt ttacggtgac gttcctccgt gatcccggtc gagagccagg
     1081 cagagacgat ctcgcgcatc tctgcgggcg tgatcgtgcc gcttccgagc gtgagcacgt
     1141 tggcgtcgtt gtgttcgcgg gaattgacgg cgacacggac ggagtagcag gcggcggcgc
     1201 gcacgcccgg caccttgttg gccgtcatac atgagccgac gcctgcgccg tcgatgatga
     1261 tgccgagatc gcattcgccg tcggcgacgg cgcgtgcgac ggcatgtgcg aagtcgggat
     1321 agtcaacggc gtcggtcgag ttggtgccga agtcgcgcac gcgctggccg agttcggcaa
     1381 ggtaagtttt gagttcttcc ttgagagggt agccgccgtg atccgcgccg acggcgatag
     1441 acttgctttc gcgcgagccg cgacgcgaag ggcgacggac gagttcgatg ccccgttcac
     1501 gcacgatgtc agcggcgagc ggcgtgagac gcgcgccctc cgcgatgcgc aggcgcgcgc
     1561 cgcgttcgag gccgtgcacg tccgcctccg tgataaccgt ctttgccgat tcatcacggg
     1621 cgataggctt atcctccggc gcgctcgtcg cagactttgc gcctgccggg ggcggcgcga
     1681 atgtcgttgc gcgcggcggc gcggcggcgg gcgttgtttt aggcggcgca ggggcggcgg
     1741 caggaggagg ggcgggcgcg ctcgcgtatg aaggcgacgc tgtttgagat gcgtccgctc
     1801 tctctccgct ctcttcttcg gacagtgcgt tgttgagcac ctcgcgcacg agggcgcgga
     1861 cgcggtcgcg tgtggattgt tcgtcaggca taagattgct gtcggctgtc agtcgttggc
     1921 agttcgccgg gcgctggctg aaagctgata gctaacggct attagccagc ggcttggcac
     1981 ttcattgacc tgtggcgagc ggctacttta gtatgtcgca ccaagaggcg cgcgtgcaaa
     2041 ccgcgtcgct cgtcggtcgc acttttaagt tcaaaaattt tccgtcgcac aataggagtc
     2101 cgccacgtgt cagcttcttc cgcactcatc cccagcgaat tcacaaacga taatctcgaa
     2161 ccgctcttca gcgcggaaga gattcaaaac cgcatcacct cgctcggcgc ggagatcgcg
     2221 cgcgactacg cggggcgcaa cccgctgctg ctcggcgtcc tcaaaggcgc ggttatcttt
     2281 ttgagcgata tgatgcgtgc gattgatttg cggctcggcg tggagttcat ggcgatctca
     2341 agctacggcg aagcgacgcg ctcgtcgggc gaagtcaaga tcgtcaaaga cctcgacgtg
     2401 ccgattgaag ggcgcgacat cctgatcgtc gaagacatcg tggatacggg gctgacgctc
     2461 tcttacctga tggcgaattt acgttcgcgc ggcgcggcga gcgtcaaact cgtcgccctc
     2521 ttgaacaaac aggagcggcg tatccgcgaa gtgcagatcg attatctcgg cttcaccatc
     2581 ccagacgctt tcgtcgtcgg ctacggcctc gacttcgccg aacgctaccg caacctcccc
     2641 tacatcgccg tcatcaaaga cccctcaaaa cagtgatgag caaagaaaag tgatgagtga
     2701 tgagtg
//
LOCUS       DASWNS010000025         9565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_104384, whole genome shotgun sequence.
ACCESSION   DASWNS010000025 DASWNS010000000
VERSION     DASWNS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104384"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VGB76_00700"
     CDS             <1..1077
                     /locus_tag="VGB76_00700"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY0097443.1"
                     /translation="TGTQARAVLDNARTAAGAGGTFNIMFLQALGSVLPMGTMQVLLF
                     FAFAIGFAIKVPMWPFHTWLPDAHVEAPTAGSVILAGILLKLGTYGFFRFNLPMFPEA
                     SKDLSYFNLFGATFGVRHIMVSLAIIGIIYGALAAMYFVVVKDGDVKKLVAYSSVSSM
                     GIVMLGLFALNPNGLNGAVLHMINHGIYSAALFLLVGIIYERRHTRKVSEFGGLSHVM
                     PGYATVFLAMVMTAIGLPLLCVFISEFLAMRGAFEAQPWWAAWAALGIILNAGYMLWL
                     YQRMFFGHVDNPKNENLTDMNGREWAYMLPLVVLSLWIGVYPKPFIEYIQRPVNAVVR
                     QVRPDYPLQPGAPATAPTTARVER"
     gene            1156..2730
                     /locus_tag="VGB76_00705"
     CDS             1156..2730
                     /locus_tag="VGB76_00705"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY0097444.1"
                     /translation="MFLQANTTALFKMRELELIVPELILTLVACGVLVMEVMLPRRKS
                     KWTGYFALVGCALAVVSLVLLWRTHRAGLPLDAFYGMVRLDGFALVFKLIFLLASALT
                     IAVSLRYLDVEGEQRGEYYALILFATVGMMFLAGGYDLIVLFISLELMALTFYVLVGF
                     TKRERRSNEAGMKYFLLGAFSSGVLLYGMSILYGVAGSTNLGDIARSVDAIMNAPPVE
                     GALAGGLPLRPLLLLGMIALAAGLFFKIAAVPFHMWAPDAYEGAPTSVTAFLSTGSKA
                     ASFALLARIFLEALGGMRADWAPLLGLVAAVTIMVGNWAAVTQTNAKRLLAYSSISNA
                     GYLLLGVIAANRYGYIGLVVYLFVYTLMNIGAFGVIISLRRRGIVGDMVDDFTGLAQK
                     APGMALMMTIFMLSLGGLPLTGGFIGKWFLFGGLLQRYDAGEKSWYLWLAIWALLNTV
                     VSFFYYIRFIRVMYLGEGTADARPLSLSPALRTALIVSVIGVIVIGVYPQPFINLAQT
                     LVASLSSVASGTFALK"
     gene            2774..3055
                     /locus_tag="VGB76_00710"
     CDS             2774..3055
                     /locus_tag="VGB76_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097445.1"
                     /translation="MKRTEMVLNRLTIALALATQLFLFAPATQAVSNTTPSATPGMQH
                     ESHNMQPTRRNRCRERCLREYRQCTRGVANPSQARCRQRYNQCLRRCRR"
     gene            3112..4383
                     /locus_tag="VGB76_00715"
     CDS             3112..4383
                     /locus_tag="VGB76_00715"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0097446.1"
                     /translation="MGFYYLFAALLLLQALISLRGGARYLRYFRRELSAPRSVYTPYA
                     SVIVPCRGLDQDLHKNLDALYQQHYPSFEIIFVVDSLEDPALEIIERVRAERGSSASV
                     PARVVVAGRANGCGQKVHNLIAAVRAVDERSEVLVFVDTDARPRRDWLRSLVAPLTDE
                     KTGAATGYRWFIPSNDLASRHKLASHLRAVWNASIASALGENRRANFCWGGSTAIRRT
                     TFAALDMPERWRGTLSDDFALTRALQDARLHIRFVPHCLTPSYEDCSGRELFEFTTRQ
                     LKITRVYAPRLWQVVLVSNLLFVAVFYGGLALSLARFWRGLPTALPLAFVAAIYVLGS
                     CKALLRLRAVALAFGGEHKIWRAVTICAHLSLWPLASALFLYNALAAARSRRIRWRGI
                     VYELKSPTETGIIDAARFEDTTADKKRVRRA"
     gene            4389..5510
                     /locus_tag="VGB76_00720"
     CDS             4389..5510
                     /locus_tag="VGB76_00720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0097447.1"
                     /translation="MTNKAVKLFQLLRSTERERRLGTVILFVTSRCNAKCATCFYWDE
                     LNQPGDMTFAQLERVSQTMPPITDLWLSGGEPTLRRDLNDIINLFVRGNGVRRVIIPT
                     NAIIKAHVCEAVGRALGDNPEIDLYLNVALDGYGSTHDRIRGVPGNWEKTLDCIRSLY
                     PLKERYGDRFRLNVNTVVCAENYTEIERLSVYLWENFQLDGHYFNLIRGETKAGATIK
                     QVPTEILPEMYARVSRLTKRYGERMFAEDEAATRFVKSVAYVGAITTHYRTQLANFER
                     QTAWAFPCTAGETTAVIDYNGDVRACELREKFATLSDYDYDFGALWNSQHRSAELAAI
                     DGGNTCWCTHVCFIHDSMRHSRRALLYELPKNYATREKW"
     gene            5616..5831
                     /locus_tag="VGB76_00725"
     CDS             5616..5831
                     /locus_tag="VGB76_00725"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY0097448.1"
                     /translation="MSNKDDQKVNRKSFALIGAVTVLSSSILSCLLLGWVLDRWLGTT
                     PWLIVAGIIIGSVAGFMHLIRVMSRIS"
     gene            5889..6344
                     /locus_tag="VGB76_00730"
     CDS             5889..6344
                     /locus_tag="VGB76_00730"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY0097449.1"
                     /translation="MNEIANSGALDGEMSGEVASGDASAMAERLFRLMTAAVLVAVVV
                     SALWLPWRVTSGLFIGGALSLLNHHWMRTAIMAAFASAANAGAKPQLKIVRFITRYFI
                     VAVAITLAHWLDIASLTAMLVGLCSFVVAALVEGFMQVYFVIIHREETK"
     gene            6344..7348
                     /gene="atpB"
                     /locus_tag="VGB76_00735"
     CDS             6344..7348
                     /gene="atpB"
                     /locus_tag="VGB76_00735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY0097450.1"
                     /translation="MFWLLQGHGEDAAHGATAGHAETATAAGAHGGGEAAAHHTPPLV
                     EAVNYFIGRPVHNFQMKYTKPLWDKFFHYFGTDAESVFGLYTVNNAVPWYTVMFIVAC
                     LITVALIWILKGKYLSEDEPHGGQQTLEVTVLAVRDMVENIIGPHGLKYFPVVATFAL
                     LILVSNLMGLFPMFMPPTAHTSVTFALGITSFIYYNYIGVKENGLMGHLGHFAGVHYF
                     SGAILFIMGPAMFVIELISNAVRPLSLGLRLFGNIFGDEQVAVNIAGIYPIVTHWGIP
                     AFLMILSVFASIMQAFIFTLLSMIYIGEVSHAPHEEHEDEHGNQLQPIEGNEIIAPAL
                     "
     gene            7734..8063
                     /locus_tag="VGB76_00740"
     CDS             7734..8063
                     /locus_tag="VGB76_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEY0097451.1"
                     /translation="MNKLRMAFFAVLGVLMSAAPALAQAAEVAADNASSVAKYSKLAA
                     GFGFAIAAGLGAIGQSRVAAAAAEGTARNPGAGGRIQILMIIGLAFIETLVIFTLVIV
                     FVAVRVA"
     gene            8357..9526
                     /locus_tag="VGB76_00745"
     CDS             8357..9526
                     /locus_tag="VGB76_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097452.1"
                     /translation="MRKIILVLLLLVGCAPYALADTIYLRDGRTVRGTVLGFIGGRFA
                     VRVATATGTSTTTASGVAAEQGEVQFFRPNQIERIEIEGRSLDEARFSTRNVEVGLDA
                     NWIDSGVDVRRGERLQLKATGTIVAGRARITPGGLRSTDPTAPLPRAAEGVLIGAIGN
                     EPDSPIIEVGIGREFVADRDGRLYLTANRGTYTDARGSYSVQIRTERNLTPRRRDNTA
                     LRNNNDEELDDDLFGTRQTSPAPVRSRRRTGDNPDTSDPNSSGNNREPREVTVDVPGT
                     SRGIDTGIELRTGDQVSVSASGTVVAGRRAGQVSPDGGRVSGLGIVSAYPVPNAGVGA
                     LIGYIRLTSGQTTAPFILGSQQSFAAQADGRLFLLVNDDNYSDNSGNFTVRIRVN"
BASE COUNT         1767 a         2983 c         2791 g         2024 t
ORIGIN      
        1 acgggcacgc aggcgcgcgc cgtgctcgat aacgcgcgga cggcggcggg cgcgggcggc
       61 acgttcaaca tcatgttcct ccaagcgctc ggctccgtcc tgccgatggg cacgatgcag
      121 gtcttgctct tcttcgcctt cgccatcggc ttcgcgatca aagttccgat gtggccgttt
      181 cacacgtggc tgccggacgc gcacgtcgaa gcgccgaccg caggctccgt gatcctcgcg
      241 ggcatcctgc tgaaactcgg tacttacggt ttcttccgtt tcaacctgcc gatgttcccc
      301 gaagcgtcga aggacttaag ctacttcaac ctgttcggcg cgaccttcgg cgtgcgccac
      361 atcatggtca gcctcgcgat catcggcatc atctacggcg cgctcgccgc gatgtacttc
      421 gtcgtcgtga aagacggcga cgtgaagaag ttggtcgcct attcgtccgt ctcttcgatg
      481 ggcatcgtca tgctcggcct gttcgctctg aacccgaacg gcctgaacgg cgcggtgctc
      541 cacatgatca atcacggcat ctattccgcc gcgctcttcc tcctcgtcgg catcatttat
      601 gagagacgcc atacgcgcaa agtctccgag ttcggcggac tctcgcacgt catgccgggc
      661 tacgccacgg tctttttagc gatggtgatg acggccatcg gcctgccgct gttgtgcgtc
      721 ttcatctcgg aattcttagc gatgcgcggc gcgttcgagg cgcagccgtg gtgggcggcg
      781 tgggctgcgc tcggcatcat cttaaacgcg ggctacatgc tctggctcta ccaacgcatg
      841 ttcttcggcc acgttgacaa cccgaagaac gaaaatctaa cggacatgaa cgggcgcgaa
      901 tgggcttaca tgctcccgct cgtcgtgctc tcgctctgga tcggcgtcta tccgaagccg
      961 ttcatcgaat acatccaaag acccgtcaac gccgtcgtcc ggcaggtgag gccggactac
     1021 ccgcttcaac ccggcgcacc cgctacggcg ccgacgacgg cgcgggtgga aagataaaag
     1081 ggatgaggga tgagggatga ataaaacagt cgtcttaact cgtcacttac cgcttgtcgc
     1141 tcgtcactcg taactatgtt tcttcaagcc aacaccaccg ccctgttcaa aatgcgcgag
     1201 ctagagttga tcgtgccgga gttgatcctt acgctcgtgg cctgcggcgt gctcgtgatg
     1261 gaagtcatgc tgccacgacg caagagcaaa tggacgggct acttcgcgct cgtcggctgc
     1321 gcgctcgccg tcgtctcgct cgtcctgctc tggcgcacac accgcgcggg cttgccgctc
     1381 gacgctttct acgggatggt acgcctcgac ggttttgcgc tcgtcttcaa attgatcttt
     1441 ctgctggcct ccgcgctgac catcgccgtg agcctccgct atcttgacgt agagggtgag
     1501 caacgcggcg agtattacgc gctcattctg ttcgccaccg tgggcatgat gtttctcgcc
     1561 ggtggctacg atctgatcgt gctcttcatc agtctggaat tgatggcctt gacgttctac
     1621 gtgctcgtcg ggtttacgaa gcgcgagcgg cgctcgaatg aagcgggcat gaaatatttc
     1681 ctgctcggcg cgttctcctc cggcgtgttg ctctacggca tgtcgatcct gtacggcgtc
     1741 gccggttcga cgaacctcgg cgacatcgcg cgcagcgtcg acgcgattat gaacgccccg
     1801 cccgtcgaag gcgcgctcgc cggaggactg ccgctgcgtc ctctcctgct gctcggcatg
     1861 atcgcgcttg ccgcagggct gttcttcaag atcgccgccg tgccctttca catgtgggcg
     1921 ccagacgctt acgagggcgc gcccacttcc gtcacggcct ttctctccac gggatcgaag
     1981 gccgcgagct tcgcactcct ggcgcgcatc tttctcgaag ccctcggcgg gatgcgtgcg
     2041 gactgggcgc cgcttctcgg cctcgtcgcc gccgtcacga tcatggtcgg caactgggcg
     2101 gcggtcacac aaacgaacgc gaagcgtctg ctcgcctatt cgtcaatttc caacgcgggc
     2161 tacctgctgc tcggcgtgat cgcggctaac cgctatggct acatcggcct cgtcgtctat
     2221 ctcttcgtct atactttgat gaacatcggc gcgttcggcg tcatcatcag tctgcggcgg
     2281 cgcggcatcg tcggcgacat ggttgacgat ttcaccggac tcgcgcagaa agctccgggc
     2341 atggccctga tgatgaccat cttcatgctc tcgctcggcg gcctgcctct gaccggcggc
     2401 tttatcggca agtggtttct cttcggcgga ctgcttcaac gctacgacgc gggcgaaaag
     2461 agttggtatc tctggctcgc catctgggcg ctcctcaaca cggtcgtctc cttcttctac
     2521 tacattcgct tcatccgcgt aatgtatctc ggcgaaggca cagccgacgc gcgaccgctc
     2581 tccctgtcgc ccgcgctgcg caccgcactc atcgtctccg tgatcggcgt gatcgtgatc
     2641 ggcgtctacc cccagccgtt tatcaatctc gcgcagacgc tcgtggcgtc gctctcaagc
     2701 gtcgcctccg gcacgtttgc tctgaaatag aagcctcgct ccggcaatgc ctcacttcaa
     2761 ggagatacgc aacatgaaac gcacagagat ggtcttgaac cggctaacca tcgcgttagc
     2821 tctggcaact caactcttcc tgttcgctcc ggcgacgcag gctgtcagca atacgactcc
     2881 gtccgccaca cccgggatgc agcacgaaag tcacaacatg cagccaacgc ggcgcaatcg
     2941 ttgccgcgag cgttgcctga gggaatatcg ccagtgcacg cgcggcgtcg ccaatccgtc
     3001 gcaggcgcgt tgccgccagc gatacaacca gtgcttgcgc agatgccgcc ggtaagttcg
     3061 cgcgcaaatt tgcgaaaggc gatgctctcg tgagagcatc gcctttcgtc tatgggcttc
     3121 tactatctct tcgccgcgtt gctcctcctg caagctctca tctcgcttcg cggcggagcg
     3181 cgctatctca gatacttccg gcgcgaactc tccgcgcccc gctccgtcta cacgccgtac
     3241 gcttccgtca tcgtcccctg tcgcggcctc gatcaggatt tacacaaaaa cctcgacgcg
     3301 ctttaccagc aacactatcc gtctttcgag atcatcttcg tcgtcgatag cctcgaagac
     3361 cctgcgctcg aaatcatcga acgggtgcgc gcggagcggg gaagttccgc gtctgtcccg
     3421 gcgcgcgtgg tggtcgcggg gagggcgaac gggtgcggcc agaaggtcca caatctcatc
     3481 gccgccgtgc gcgcggttga cgagcgcagc gaggtcttag tcttcgtcga taccgacgcg
     3541 cgaccgcgcc gcgattggct gcgctcgctc gtcgcgccgc ttacggacga gaagacggga
     3601 gccgcgaccg gctatcgctg gttcatcccg tcaaacgatc tcgcctcgcg ccataaactg
     3661 gcttcgcacc tgcgcgccgt ctggaacgcc tccatcgctt ccgcgctcgg cgaaaacagg
     3721 cgcgccaact tttgctgggg cggttcgacc gcgattcgcc gcacaacctt cgccgcactc
     3781 gacatgccgg agcgttggcg cggcacgctc tccgacgact tcgccctcac tcgcgcgcta
     3841 caagacgcgc gcctccacat ccgtttcgtc ccccattgcc tgacaccctc atacgaagat
     3901 tgctccgggc gtgagctttt tgaatttacg acgcgccaac tcaagatcac gcgcgtctac
     3961 gcgccccgcc tgtggcaagt cgtgctcgtc tcgaatctgc tcttcgtcgc ggtcttctac
     4021 ggcggcctcg cgctctccct cgcgcgcttc tggcgcggcc tgcccacagc tttgccgctc
     4081 gccttcgtcg ccgcgatcta cgtgcttggt tcgtgcaaag ctctcctgcg cctgcgcgcc
     4141 gtcgcgctcg ccttcggagg cgaacacaaa atttggcgtg ccgtcaccat ctgcgcacac
     4201 ctctccctgt ggccgctcgc atccgcgctc tttctctaca atgcgctcgc cgccgcgcgc
     4261 tcgcgtcgca tacgctggcg cggcatcgtc tacgaattga aatcgccaac ggaaactggc
     4321 atcatcgacg ccgctcgatt cgaagacacg acggcagaca agaaacgcgt gcgccgcgcc
     4381 tgaaggtcat gacaaacaaa gcggtcaaac tttttcagtt gctgcgctcg accgagcgtg
     4441 agcggcgtct cggcacggtg atcctgttcg tcacctcgcg ctgcaacgcg aagtgtgcga
     4501 cctgttttta ctgggacgaa ctcaaccagc cgggcgacat gacatttgcc caactcgaac
     4561 gcgtttcgca gacgatgccg cccatcacag acctctggct ctcaggcggc gagccgacgt
     4621 tgcgccgcga tctaaacgac atcatcaatc tcttcgtgcg cggcaacggc gtgcgccgcg
     4681 tcatcatccc gaccaacgcc atcatcaaag cgcacgtctg cgaggcggtc ggacgcgcgc
     4741 tcggcgacaa tcccgaaatc gatctctatc tcaacgttgc gctcgacggc tacggctcca
     4801 cccacgaccg catacgcggc gtgcccggca attgggagaa gacgctcgac tgcatccgct
     4861 cgctctaccc tctgaaagag agatatgggg atcgctttcg cctcaacgtc aacacggtcg
     4921 tctgcgcgga gaactatacg gagatcgaac gactctccgt gtatctctgg gaaaacttcc
     4981 aactcgacgg ccactacttc aatcttattc gcggcgagac gaaagcgggc gcgacgatca
     5041 agcaggtgcc gacggaaatt ttgccggaga tgtacgcccg cgtgtcgcgc ctcacgaagc
     5101 gttacggcga acgcatgttc gcggaggatg aagccgccac gcgcttcgtc aaatccgtcg
     5161 cctacgtcgg cgcgatcacg acgcactatc gcacccaact cgccaacttc gaacggcaga
     5221 ccgcgtgggc tttcccctgc acggcgggcg agacaactgc cgtcattgat tacaacggcg
     5281 acgtgcgcgc ctgcgagtta cgcgaaaaat tcgccaccct ctccgattac gattacgatt
     5341 tcggcgcgct ctggaacagc cagcatcgca gcgcggaact cgccgccatt gacggcggga
     5401 acacctgctg gtgcacgcac gtctgcttca tccacgactc catgcgccac tcgcgccgcg
     5461 cactcctcta cgagttgccg aagaactacg cgacgcggga gaaatggtga actatcgcta
     5521 gttccttgtc agttgtcagt tgttcgagca aaaattcggt gattcaacgg atagccgagt
     5581 gcaacgaaca acggataact gacagcttca aagttatgtc gaacaaagac gaccagaaag
     5641 ttaatcgcaa gagtttcgcg ctgatcgggg cggtgacggt gttgtcttct tccatcttgt
     5701 catgcctgtt attgggttgg gtgctggatc gctggctggg caccaccccg tggctcatcg
     5761 tggccgggat catcatcggg tcggtggccg gtttcatgca cctcatacgc gtcatgtcgc
     5821 ggatttcgtg aaggattatc gccgctcgac agcccgccgc cggagcatgt agaatctagt
     5881 gttctacgat gaatgaaatt gcaaacagcg gcgcgcttga cggtgaaatg tcaggcgaag
     5941 tggcgagcgg agatgcgtct gcgatggcgg aacggctgtt tcgcttgatg accgcagcag
     6001 ttctcgtcgc cgtggtggtc agcgcgttgt ggttgccgtg gcgcgtgacg agcgggttgt
     6061 tcatcggcgg cgcgctttct ctgctcaatc accattggat gcggacggcc atcatggcgg
     6121 cgtttgcgtc ggcggcgaat gcgggcgcga aaccgcaact caagatcgtg cgcttcatca
     6181 cgcgctactt tatcgtcgcc gtcgccatta cgctggcgca ctggctcgac atcgcctctt
     6241 tgacggccat gctcgtcggc ctctgttcgt tcgtcgtggc tgcgctcgtc gaaggcttca
     6301 tgcaggttta tttcgtgatc atacatcgag aggaaactaa ataatgttct ggttgctgca
     6361 aggacatggt gaggacgcgg cgcacggcgc gactgccgga cacgccgaga ctgcaacggc
     6421 ggcgggtgcg cacggcggcg gcgaagcggc agcgcaccac acgccgccgc tcgtcgaagc
     6481 ggtcaattat ttcatcggca ggccggtgca caatttccag atgaaataca ccaagccgct
     6541 ctgggataag ttcttccact atttcggcac tgatgccgag tcggtcttcg gcctctacac
     6601 cgtgaacaac gccgtgccct ggtacacggt gatgttcatc gtggcgtgtc tcatcacggt
     6661 cgctttgatc tggattctca agggcaagta tttatcggag gacgaaccgc acggcgggca
     6721 gcagacgctg gaagtaacgg tgctggccgt gcgcgacatg gtggagaaca tcatcgggcc
     6781 gcacggcttg aaatactttc cggtcgtcgc cacgttcgcg ctcttaatcc tcgtctcgaa
     6841 tttgatggga ctcttcccga tgttcatgcc gccgacggcg cacacctccg tcaccttcgc
     6901 gctcggcatc acgtcgttca tttactacaa ctacatcggc gttaaagaga acgggttgat
     6961 gggacacctc ggccacttcg ccggagtgca ttatttcagc ggcgcgatcc tcttcattat
     7021 gggtccggcc atgttcgtca tcgagttgat cagtaacgcc gtgcgcccgc tctctttggg
     7081 gctgcgtctc ttcggcaaca tcttcggcga cgaacaggtg gcggtcaaca tcgccggaat
     7141 ttatccgatc gtgacccact ggggcatccc cgcgttcctg atgatcctca gcgtcttcgc
     7201 ctccatcatg caggcgttca tctttacgct gctttcgatg atctacatcg gcgaggtttc
     7261 tcacgcgccg cacgaagagc acgaggacga gcacggaaat caacttcagc cgattgaggg
     7321 caacgagatc attgcgccgg cattataaga gcagtgacaa gtgataagtg ataagtgacg
     7381 agattgaaga cagttgactg gttttaactt gtcacttgtc actcgtcact gtagttgaaa
     7441 gcgttctgag cggtgcgggg agacgcgcct gcacgcgttg gggcgtttgg atgggaaact
     7501 ccgggaggga gaaaatcgtt aaggataagt gaggggcgaa gaggcacgcg gagaatcata
     7561 gctttcgctg tttgaaagta aacgggtgtc ggcatcgctg gagcgccgtt gtaagagggg
     7621 cggcggatac cggatgcggg cgaaatcata agtggcgcgt gcagcgtttc aattgtccgg
     7681 cggagttcaa cttcctctct gttgataatc aactctgaaa aggagagtcg aatatgaata
     7741 aactcagaat ggcattcttc gcagtgctcg gcgtgttgat gtctgccgcg cccgcgctcg
     7801 cgcaggccgc cgaagtggcg gcggacaacg cttcgagcgt tgccaagtat tcgaaattgg
     7861 ctgccgggtt tggtttcgcg attgcggcag gcttgggcgc gattggacag tcgcgcgtgg
     7921 cggcggcggc ggcggaaggc acggcgcgca atccgggcgc gggcggtcgt atccagatct
     7981 tgatgatcat cggtctggcg tttatcgaaa cgctggtcat cttcacgctg gttatcgtct
     8041 tcgtggctgt tcgcgtggcg taactgcggc gagactgtaa acaccagaat gctttgcggg
     8101 cgcgtctctt ttcaatcgag acgcgcccgc aaactttttt atgcgtcgcc ttgtgcttgc
     8161 ctgccaatcc gcttcgttgt agtctctaga cacattcccc cgggttaatc caaagttatt
     8221 catccccttc tgtaattctc tttcgtgagt gattggcggc tggcaaactt gtactctgtc
     8281 aaagctgcgt ttgaattgaa tttgacgaac cctgagtgct ctcttaaaac tctggctgtg
     8341 agaggatggg aaaaaaatga gaaagataat cttagtctta ctgttactcg tcggatgcgc
     8401 gccgtacgct ctggccgaca cgatctattt gcgtgatggc cgcaccgtgc gtggcacggt
     8461 gctcggcttc atcgggggac gtttcgccgt gcgcgtcgcc acggcgacgg gcacgagcac
     8521 gaccactgcg agcggagttg ccgcagagca gggcgaggtg cagttctttc gtccgaatca
     8581 aatcgagcgc atcgagatcg aaggccgctc gctcgacgaa gcacgcttct cgacgcgcaa
     8641 cgtcgaagtc ggcctcgatg caaattggat tgacagcggc gtggacgtgc ggcgaggcga
     8701 acgcttgcag ttgaaagcca ccggcaccat cgtcgccggg cgcgcacgca tcacgccggg
     8761 cggtttgcgt tcgaccgacc cgaccgcgcc tctgccgcgc gctgccgaag gcgtgctcat
     8821 cggcgcaatc ggcaacgagc ctgactcgcc gattatagag gtcggcatcg ggcgcgagtt
     8881 tgtcgccgac agggatggcc gcctctacct gactgccaat cgcggcactt acacggacgc
     8941 acgcggttca tactccgtgc agattcgcac cgaacgcaat ctcacgccgc gcaggcgcga
     9001 caacaccgcc ttgcgcaaca ataatgatga ggaactcgac gatgacctgt tcggcacgcg
     9061 gcaaacttca cccgcccctg tgcgctcacg caggcgaacc ggcgacaacc cggacacgag
     9121 cgatccgaac agcagcggca acaaccgcga accgcgcgaa gtgacggttg acgtgcccgg
     9181 cacttcacgc ggcatagaca ccggtatcga actgcgtaca ggcgatcagg tatcggtcag
     9241 cgcgtcgggc actgtcgtgg cggggcggcg cgcggggcag gtttcgccgg atggcggtcg
     9301 tgtcagcgga ctcggaatcg tcagcgcata cccggtgccg aacgcgggcg tcggcgcgct
     9361 catcggctac atccgtctga cgagcgggca gaccaccgcg ccgttcatcc tcggcagcca
     9421 gcagagtttc gccgcgcagg ctgacggtag attgttcctg ctcgtcaacg acgacaacta
     9481 tagcgacaac agcggcaact tcaccgtccg catcagagtc aactaagaca gtgacgagtg
     9541 acaggtgaca ggtgacaagt taaag
//
LOCUS       DASWNS010000026         6027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_130614, whole genome shotgun sequence.
ACCESSION   DASWNS010000026 DASWNS010000000
VERSION     DASWNS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130614"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1182)
                     /locus_tag="VGB76_00750"
     CDS             complement(112..1182)
                     /locus_tag="VGB76_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097453.1"
                     /translation="MSKEPQVRDVVPFKSGKRPTTARKLNLTFAGGTALEEALAGQEK
                     RTNHDSPPLPTTPHHSPDNIAPKKDFNRRANSLDRDALPAGLFRGSSKKLYDALYLRS
                     RGAITPKRHVRASRRELLEWTGIRNLKTIDSHIHYLMAVGLIQRHWELGSTEGSEYEV
                     LLPEELDSPRLTTTHHHSPPDTTSQKMSSGNTQFLGSGGDSQTIDNAATSEASKTDLR
                     LDQKNIDDEAFAMLCEKLQAVSSELTGKQTTTADRARWGEVADVLIAELRIASGRTTV
                     SSVPAFLAEHLRRRLWKIDRKQAQAEGRELPDQPQSKNLLPQGQTCPDCNNTGWWYPH
                     GTEKGVARCKHANLSDSAATEK"
     gene            complement(1157..2143)
                     /locus_tag="VGB76_00755"
     CDS             complement(1157..2143)
                     /locus_tag="VGB76_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0097454.1"
                     /translation="MVSSEFISATEACEFLGISPDTFRRIVRDMNLPTTSDPTDARVK
                     LYNRAAITALKQQPLIIPTRHRVITLSNNKGGVSKTTSAISLAAEAAADGLKVLLIDA
                     APQASATISLLDEAERPQETDHGILLSWLKGDTPFQELITPIVFRQFQIDFIPSSPKN
                     DQIDRANPLEAVPALREFFESWDDSPYDYIFIDTDPSFGTLVSMAQVGSHFVICPVQA
                     DVLSVDGTIQLTRQLQRARNLTKSQFPALLGFFLARFDPRKRVCKEALDTLRAAYPES
                     VFEAVIPENVRVTESPSQKAPINLTAPDSKGAIAYKALWKEVRERVERTTGA"
     gene            2145..2849
                     /locus_tag="VGB76_00760"
     CDS             2145..2849
                     /locus_tag="VGB76_00760"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0097455.1"
                     /translation="MDTPYYGTRSSKSLRSISQHVCILCPHNLGVNDYLLEGQDSNVL
                     TCVYLIDNKWSVAASVEVSERGTAKEAGGRFRQVGHEIVDHFSHSNGFHRWFLMLCSG
                     ALFLDTVWAKGRLQMITVANRIYVKREYAEAFEARFRERAGLVDKMHGFVSNQVLRPV
                     NEGDPYVVFTTWHSRQDFLNWVRSDEFVRGHAQSGTLPKEAFSGGNVLEMHEVIQDSS
                     RPDLEPEPAGGPFKMH"
     gene            3057..4244
                     /locus_tag="VGB76_00765"
     CDS             3057..4244
                     /locus_tag="VGB76_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY0097456.1"
                     /translation="MYLKPKAAQALFIVLLLCHTLFAAPSVRWTNARANEWYRQQPFL
                     VGSNYNPATAINELEMWQAETFDPKRIDLELGWAENIGMNTMRVYLHDLPYKQDAEGF
                     KRRINAFLQIADKHKIRPMFVLFDSCWDPFPVAGKQREPRPGVHNSGWMQSPGKVALE
                     DPAEYPRLEAYVKGIVGAFKDDKRILAWDIWNEPDNMNDPAYISLEPKNKVELVLALL
                     PQAFQWAREAGATQPLTSGVWKGGDWSNPRELTAMEITQLYLSDIISFHNYDDAESFE
                     RRIKQLQRYDRPILCTEYMARGNGSTFEGSLPIAKRYNVAAINWGLVQGRSQTHLPWD
                     SWRRPYVDREPAIWFHEVFRNDGTPYRPQEVELIKRLTGKVRTARVNRQTSRPIGAPV
                     AFR"
     gene            4259..>6027
                     /locus_tag="VGB76_00770"
     CDS             4259..>6027
                     /locus_tag="VGB76_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0097457.1"
                     /translation="MGKLIKRLRALLLRAGSDRELDEETHFRLERQIESNISGGMSPL
                     EAPQAALRSFGGMEQAKESCREARGVRLIEDLRQDLRYGLQMLLKNPGFTLVAVLSLG
                     LGIGLNTTVFSFINSIMFRPLALPDPGKLVRIHDDNIPAFSDYLAFRERADVFSGLAA
                     YDFDNFNLLSGASSGRVGVVKVSGNYFEVLKVRMALGRSFSTEEDRPGGHPVAVLSYG
                     LWQSRFGGDQNIVGKSFILQREAFTVIGVVPREFQGVALGWRHDLWIPIAMDARLHPE
                     HNLLKHPDSYQVRVIGRLKPEVELAQAQAVVKTIAATQDKITKVRLFTDSPDEQPPTP
                     SALLPATMIEISPRDRRQAWLGIFGVMAVMGSVLLVACANVANLLLGRATKRRREIAI
                     RLALGAGRRRIVRQLLTESLLLSLIGGALGLLLARWAADILLSILPRVAPREDASIFL
                     DLSLDWRILIFTLLLSLVTGIAFGLVPALQTSKPDVMSALKDETPTLAGRGRRFTFRN
                     ALVVTQVAVSFLLLIPSGLLIRNMRLAQSSDHGFATGHRYVMPVDLEGLGYDENRQRS
                     YRNALLESIRAVPGIRSATLADIV"
BASE COUNT         1275 a         1632 c         1721 g         1399 t
ORIGIN      
        1 tgttcaatta tttatccttg tgaaacaacg tgaaacttgc ctgtcgccct tccgccttct
       61 ctcaactctc agggacaaag ctgctacttc tatctgcctg tcgcgcctaa cttacttctc
      121 tgttgccgca gagtcagaca agttcgcatg cttgcagcgt gcgacaccct tctccgtgcc
      181 gtgcggatac caccagcctg tgttgttgca gtcaggacaa gtctgaccct gtgggaggag
      241 gttttttgac tgcggctggt caggcagttc cctgccctcg gcttgcgcct gtttcctgtc
      301 aattttccac agacgacgac gtagatgctc ggccagaaag gccggtacgc tcgacactgt
      361 cgtgcgtccg gaagcaatcc gtaattctgc gatcaggaca tcagcgactt ctccccagcg
      421 cgccctgtct gctgtagttg tctgtttgcc tgtcaactcg gaacttaccg cttgtaactt
      481 ctcgcatagc atggcgaaag cttcatcatc aatatttttt tgatctagtc ttagatcagt
      541 cttagaggcc tcggaagttg ctgcattatc aatagtttga ctatcaccac cactccccaa
      601 aaattgggta ttcccactac tcattttctg actagtggtg tctggtgggg agtggtggtg
      661 agtcgtggtg agtcgtggtg agtctagttc ttccggcagc aacacttcat attcggagcc
      721 ttccgtactg cctaactccc agtgacgttg gatcaagcca acggccatca aataatgaat
      781 gtggctatca atagtcttga gattgcggat gcctgtccac tctagaagtt ccctgcgcga
      841 tgctcgaaca tgcctctttg gagtgatagc ccctcgtgac ctcaaataga gcgcatcgta
      901 gagtttttta ctcgatccgc gaaacagacc ggcggggagc gcatcgcgat ctaaactatt
      961 cgcgcgccgg ttgaagtctt ttttgggcgc gatgttgtct ggggagtggt ggggagtggt
     1021 ggggagtggt ggggagtcgt ggttagtcct cttttcctga cctgccagtg cttcctctag
     1081 ggcagtccca cctgcaaagg tcaggttaag ttttctggcc gtagtggggc gtttgcccga
     1141 tttgaatggg acaacgtcac gcacctgtgg ttctttcgac acgctctcgc acctccttcc
     1201 acaatgcctt ataagcaatc gcgcccttcg agtcgggagc tgtcaggtta atgggcgcct
     1261 tctggctggg actttcggtt acgcgtacgt tctccggtat gaccgcctcg aacacagact
     1321 ccgggtaggc agcccgaaga gtgtccaaag cctctttgca gacgcgcttc cgtgggtcaa
     1381 acctagccag gaagaaaccc aaaagggctg ggaactggct tttggtgagg tttctggctc
     1441 gctgcaactg cctggttagc tggatcgtgc catccacgga gagcacgtcg gcctgtacag
     1501 ggcagatgac gaaatgactg ccaacttgtg ccattgaaac caaagttcca aaggaggggt
     1561 cagtatcaat aaaaatgtaa tcgtaaggtg aatcgtccca actttcgaaa aactccctca
     1621 acgcaggcac cgcttcgagc ggattagccc tgtctatctg gtcgtttttc ggcgaactgg
     1681 gaatgaagtc gatctggaac tgcctaaaaa caatgggcgt gatcaattcc tgaaacgggg
     1741 tgtctccttt cagccaagag agcaaaatac cgtgatccgt ctcctgcgga cgctccgctt
     1801 catccagcag tgaaatcgtc gcagaggctt gcggagccgc atctatgagc agaaccttaa
     1861 gaccatccgc tgccgcctca gccgccagag aaatggccga agtagtcttg ctcaccccgc
     1921 ccttgttgtt actaagtgtt atcacccgat gacgtgtggg aatgattaat ggctgctgtt
     1981 taagggcggt gatggcagcc ctgttgtaga gtttgacccg cgcatcggtc ggatcactcg
     2041 tcgtcggaag attcatgtct ctgacaatcc ggcggaaagt atcaggggaa atccctaaga
     2101 attcgcaggc ttcggtggca gaaatgaact cggaactaac caaaatggat actccttatt
     2161 atggtactag aagcagtaag tcgcttcgct ccatctcgca gcatgtctgc atattatgcc
     2221 cgcacaattt aggagtcaat gattatttac tggaaggaca ggactcaaat gttttaactt
     2281 gtgtatattt aatagacaat aaatggagcg tggcggcatc ggttgaggta tctgaacggg
     2341 gaacagccaa ggaggcaggc gggcgtttta gacaggtcgg gcatgaaatc gtggatcact
     2401 tttcccattc gaacggattt catcgatggt ttttgatgtt atgctcaggc gctctgtttc
     2461 ttgatacggt ttgggcgaaa gggagacttc aaatgataac ggttgccaat cgcatatatg
     2521 ttaagcgaga gtacgctgaa gccttcgagg cgaggtttag ggagcgcgcg ggactcgtgg
     2581 ataagatgca cggattcgtc tccaaccaag tattacgacc tgttaatgaa ggtgatccgt
     2641 atgtagtctt tactacgtgg catagtcgtc aggactttct caattgggtg cgctcggacg
     2701 agttcgttag aggccacgcg caatccggta ctctgccgaa ggaggccttc tcggggggaa
     2761 atgtgttgga gatgcacgag gtcatacagg attcgtcgcg tccggatttg gagccggagc
     2821 ctgccggcgg cccgttcaag atgcactagg cttcgagcag agccgtgccg gtttgttgcg
     2881 ggtgttctcg cgcaggcgct attgacgact acaatgagtg acttccatcg aatcacacag
     2941 gctttgtttc aaggcgttag tcttttgcgc cctccaacta tcacgcgctg gccgtgaacg
     3001 acttcatcaa agggttgtag accacccgcg actcaagacg gagactcgaa aaaaatatgt
     3061 acctcaaacc taaagccgcg caggcactct tcatcgtcct gctgttatgc cacaccctgt
     3121 tcgccgcgcc ttcggttcga tggacgaatg cccgtgccaa cgaatggtac aggcagcagc
     3181 cgtttctggt cggcagcaat tacaacccgg cgacggcaat caacgaactg gagatgtggc
     3241 aggctgaaac tttcgatccc aagcgcatag acctcgaact cggctgggct gagaacatcg
     3301 ggatgaacac gatgcgcgtc tacctgcacg atctcccgta caagcaggac gcggaaggct
     3361 tcaagcgacg gatcaatgcg tttctacaga tcgccgacaa gcataagatt cgtccgatgt
     3421 tcgtcctgtt cgattcatgc tgggacccgt ttccggttgc aggcaagcag agagagcccc
     3481 gaccgggcgt gcacaactcc ggctggatgc agtctcccgg caaagtcgcc cttgaagacc
     3541 ccgcggaata tccgcgtctc gaagcttacg tgaagggaat cgtcggcgcg ttcaaggacg
     3601 acaagcgtat tctcgcgtgg gacatctgga acgagccgga caacatgaac gaccccgcct
     3661 acatcagtct ggagccgaag aacaaggtcg aactcgtcct cgccctgctg ccgcaagcgt
     3721 ttcaatgggc gcgcgaggcg ggcgcgacgc agccgctcac ctcgggcgtg tggaagggcg
     3781 gcgactggtc gaacccgcgc gagttgacgg cgatggagat cacgcaactc tacctctccg
     3841 acatcatctc attccacaac tacgacgacg cggaaagttt cgagcggcgc atcaagcagc
     3901 ttcagcgtta cgaccgtccg attctctgca ccgaatacat ggcgcgcggc aacggcagca
     3961 cgttcgaggg cagccttccc atcgccaaac gctacaacgt cgccgcgatc aactgggggc
     4021 tggtccaagg aagatcgcag acccacttgc cctgggattc gtggcggcgt ccttacgttg
     4081 atcgggaacc cgcgatctgg ttccacgaag tcttccgcaa tgatgggacg ccataccgcc
     4141 cgcaggaggt ggaactcatc aagcgactca cgggcaaagt tcgaactgcg cgcgtcaatc
     4201 gacagacctc cagacccatc ggtgcgcccg tggcctttcg ataagaggtt gcatagtgtt
     4261 gggcaagttg atcaagcgtt tgcgcgcact gttgctcaga gccgggtcgg atcgtgagtt
     4321 ggacgaagaa acgcacttcc gtctggagcg acagattgag tcgaacatct cgggcggcat
     4381 gagtccgttg gaggcgcctc aggcggcgtt acgtagtttt gggggcatgg aacaggcgaa
     4441 ggagtcctgt cgcgaagcgc ggggcgtcag gcttatcgaa gacctccggc aagacttgcg
     4501 ctacggcttg cagatgttgc tcaagaatcc gggctttacg ctcgtggcgg tgctctcgct
     4561 cggtttgggc atcgggctta ataccaccgt cttcagcttc atcaactcga tcatgttcag
     4621 acctctcgct ctccctgatc ccggaaagct cgtgcggatt cacgacgata atatcccggc
     4681 attttcagat tacctcgcct tccgtgaacg cgcggacgtt ttcagcggat tagcggctta
     4741 tgatttcgac aatttcaacc tgctctccgg tgcgagcagc gggcgcgtgg gggtcgttaa
     4801 agttagtggt aattattttg aagtgctcaa ggtgaggatg gcgctcggac gctctttttc
     4861 tacggaggaa gacaggccgg gcgggcatcc cgtggccgtt ctcagctacg gactctggca
     4921 gagtcgcttt ggcggggatc aaaatatcgt cgggaagtct ttcatactac agagagaggc
     4981 tttcacggtc atcggcgtcg tcccacggga gtttcaggga gtggctttag ggtggcgtca
     5041 cgatctgtgg attcctatcg ctatggacgc gagattgcac ccggaacata acctgctcaa
     5101 gcacccggac agttatcagg tgagggtcat cggcagactg aaaccggagg tggaactcgc
     5161 acaggcgcag gccgtcgtca aaaccatcgc cgcgacacag gacaagatca cgaaagtcag
     5221 actcttcacc gattcaccgg acgaacagcc gcccacgccg agcgcgctgc tcccggccac
     5281 gatgatcgaa ataagcccgc gtgatcgccg acaggcatgg ctgggaatct tcggtgtgat
     5341 ggccgtgatg ggttccgtgc tgctggtcgc ctgcgctaac gtggcaaatt tgttgctcgg
     5401 cagagccacg aagcgtcggc gggagatcgc catccgcctc gctctggggg caggtcgccg
     5461 ccgcatcgtg cggcaacttc tgacggagag cctactgctg tcgctcatcg ggggagcatt
     5521 gggactactg ctggcgcgtt gggctgcgga catcctgctc tcgatattgc cacgcgtggc
     5581 tccgagagaa gacgcgtcta tttttctcga cctcagcctc gactggcgca tactcatctt
     5641 caccttgctg ctgtctctcg tcacggggat agctttcggc ctcgttcccg cgctacagac
     5701 atcgaagcct gatgttatgt cggcgttgaa ggatgagacg ccgacgcttg cggggcgcgg
     5761 gcgtcgcttc acctttcgca acgcgctcgt ggtcacacag gtcgccgtct cgtttctgct
     5821 cttgatcccg tcgggacttt tgatcagaaa tatgcgtctg gcacagtctt ccgatcacgg
     5881 gttcgcgacc gggcacagat acgtgatgcc ggttgatctg gaaggactgg gctatgacga
     5941 aaaccggcag cgttcatacc ggaacgctct gcttgagagc atacgcgccg tgcccgggat
     6001 acgttcggcc accctcgcgg atattgt
//
LOCUS       DASWNS010000027         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_104500, whole genome shotgun sequence.
ACCESSION   DASWNS010000027 DASWNS010000000
VERSION     DASWNS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104500"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /gene="recJ"
                     /locus_tag="VGB76_00775"
     CDS             complement(<1..1522)
                     /gene="recJ"
                     /locus_tag="VGB76_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY0097458.1"
                     /translation="MQEAFSPTHQPVQWNVRGYDEAHARALAARLGLSPVVAGLLIAR
                     GVETEEAAQKLFHPSFDQLHDPSLMLGMSDAVRRVLRAVDAGEQVLIYGDYDVDGTTG
                     TVVLRRALQLLGAATRFHVPHRFTEGYGIRQDVLERAKAEGCALVISVDCGIRAHEPL
                     EWARANGLDVIVTDHHLPDDAEGSPPAFAVLNANQRGCNYPDKNLAGVGVAFKLAHAC
                     LRERGREHLIKSFLKVVAIGTVADVAPLVGENRAIVALGLTDIVNATNPGLRALMEVA
                     GCGDGRDMRCSDLGFRLGPRINAAGRMDAARAVVELFETTDEGEARRLAEHLDARNRE
                     RQVMQRAITGRAVAEYERSGDCGAVAVIAGDGWHRGVIGLAASRIAEKLARPCVVISL
                     EGDLGHGSARSVENYHLLDGLTACADLLETFGGHAQAAGLTIRRENIPELRRRLHEHA
                     SNFLIDGADCAPVFKVDAELPASALTLTLADELERLEPFGAQWPRPVFLSRNLRVVK"
     gene            complement(1525..1854)
                     /locus_tag="VGB76_00780"
     CDS             complement(1525..1854)
                     /locus_tag="VGB76_00780"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0097459.1"
                     /translation="MTDETRTEPDNSPSQRPRSPRVPVNFPVELEGRAPDGAPFKESG
                     EAVRVSRGGATLIAEATVELGAVLRLTPPFGHPLDAEVNGIWKDEEDGRQRIGVKLLD
                     PNGWFAE"
BASE COUNT          308 a          605 c          670 g          347 t
ORIGIN      
        1 ctttaactac ccgtaaattc cgcgacaaaa agacggggcg cggccactgt gcgccgaagg
       61 gttcgaggcg ttcgagttcg tcggcgagcg tgagcgtgag cgcgcttgcg ggcaactcgg
      121 catcaacttt gaagacgggc gcgcagtctg cgccgtcaat taaaaagttg cttgcgtgct
      181 cgtggaggcg tcggcgcagt tcgggaatat tttcgcggcg gatggtgagt ccggcggcct
      241 gcgcgtggcc gccgaaagtc tccagcaggt cggcgcaggc ggtaagccca tcgagcaggt
      301 gataattttc gacgctacgc gccgagccgt gcccgaggtc gccttcaagc gagatgacga
      361 cgcaggggcg cgcgagtttc tccgcgatgc gcgaagcggc cagaccgatc acgccgcgat
      421 gccagccgtc gcccgcgatg acggccaccg cgccgcagtc gccgctccgt tcatattcgg
      481 cgacggcgcg cccggtgatc gcgcgctgca tcacctgtcg ctcgcggttg cgcgcgtcca
      541 gatgttcggc cagtcggcgc gcttcccctt cgtcggtcgt ctcgaacagt tcgacgacgg
      601 cgcgcgcggc gtccattcgt ccggcagcgt tgatgcgagg accgaggcgg aatccgagat
      661 cgctgcaacg catatcgcgc ccgtcgccgc agcccgccac ttccatcagg gcgcgcaggc
      721 cggggttggt cgcgttgacg atgtccgtca ggccgagcgc gacgatggcg cggttttcgc
      781 cgacgagcgg cgcaacgtcc gcgaccgtgc cgatggcgac caccttgagg aaacttttga
      841 tcagatgctc gcgcccgcgt tcacgtagac aagcgtgcgc gagtttgaag gcgacgccga
      901 cgcccgcgag atttttgtcc gggtagttgc agccgcgctg gttggcgttc aggacggcga
      961 aggccggagg cgagccttcc gcgtcgtccg gcaggtgatg atccgtgacg atcacgtcga
     1021 ggccgttcgc gcgcgcccat tccagaggct cgtgcgcgcg gatgccgcaa tccacgctga
     1081 tgacgagcgc gcagccttcc gccttcgcgc gttcgagcac gtcctgccgg atgccgtagc
     1141 cttcggtgaa gcggtgcggg acgtgaaagc gcgtcgcggc tccgagcagt tgcagcgcgc
     1201 gacgcaggac gaccgtgccc gtcgttccgt caacatcgta gtcgccgtag atcaagacct
     1261 gctcgcccgc gtcaaccgcg cgcagcacgc gccgcacggc gtcgctcatg ccgagcatca
     1321 gcgaagggtc gtgcagttga tcgaacgaag gatggaacaa cttctgcgcg gcctcttccg
     1381 tttcgacgcc gcgcgcgatg agcagccccg cgacgacggg agagaggccg aggcgcgcgg
     1441 cgagcgcgcg tgcgtgcgcc tcgtcgtagc cgcgcacgtt ccactgtacg ggctggtgtg
     1501 tagggctgaa ggcttcctgc atctttactc tgcgaaccaa ccgttcgggt cgagcagttt
     1561 gacgccgatg cgttgtcgcc cgtcttcttc gtctttccag atgccgttga cttcggcgtc
     1621 gagcgggtgg ccgaagggcg gtgtgagacg caagaccgcg ccgagttcga cggtggcttc
     1681 ggcgatcagg gttgcgccgc cgcgactgac gcgcacggct tcgccgctct ccttgaaggg
     1741 cgcgccgtcg ggcgcacggc cttcgagttc gacgggaaag ttgacgggga cgcgtgggct
     1801 gcgtgggcgt tgcgaaggcg agttgtcggg ttcggttctg gtttcatcag tcatatctat
     1861 cttaagaccc ccttctgaat cggatcagaa aatgcacggc aactgtgcgg aaacaggggc
     1921 accggagagt
//
LOCUS       DASWNS010000028         6381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_389862, whole genome shotgun sequence.
ACCESSION   DASWNS010000028 DASWNS010000000
VERSION     DASWNS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389862"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /gene="murC"
                     /locus_tag="VGB76_00785"
     CDS             <1..1237
                     /gene="murC"
                     /locus_tag="VGB76_00785"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY0097460.1"
                     /translation="RAENVGDAQVVVRSTAVRDNNPELIEARERSIPVIARAEMLAEL
                     MRLKRDTVAVAGSHGKTTTTSMIGMVLGAAGLDPTVVVGGVVGAFGSNARLGKSDLMV
                     VEADESDRSFLMLAPTFTVVTNIDREHMDYYRDMQDMQDCFIRFVNKVPFYGASVLCL
                     DDPYVQAVIPHVKRRRITYGLSAQADVSAHNIKFDIPFGSRFTVWRGHEVMGEVEMRV
                     PGLHNVYNALAAIAISFELEAPFEKTVEGLGAYTGVNRRFQFKGEAQGVLVVDDYGHH
                     PTEIRATLAAAKIGSSGRRMVVLFQPHRYTRTQDQMEEFARSFNNADVLLVTDIYAAS
                     EDPIEGITAEALVEAVKRYGHKNTFYAGSLETAVKALLSEAHNGDMVITLGAGNVFRA
                     GEELIELLSKNGHGHGNGK"
     gene            1347..2390
                     /gene="murB"
                     /locus_tag="VGB76_00790"
     CDS             1347..2390
                     /gene="murB"
                     /locus_tag="VGB76_00790"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY0097461.1"
                     /translation="MTHSTISPRERDAAIEEIAARLGVRAERGRRFAELTSLRVGGAI
                     DWVFAPETEEAAAQLAGELDAAGIAWRVLGAGSNVLADDEAHHYVVLSMREVKGTALF
                     EGERVSVSAGYSLPRLCVDAARRGLAGIEGLGGIPGTVGGALWMNAGAFGHEIGNVVE
                     TVRVARRGRVVEVRGSEIEWNYRHTSFREGELLLGVTLRLRPDDSAKIVERMAEVKRQ
                     RMETQPHGARSAGCFFKNPPGSGLSTGRMIDEMGMKGEKRGGAVVSPVHANFIVTEGD
                     DARASDAFALAEEIRERIRRERGIELEYEVELWRSGNARQAVESNAGRDEAGSGEESV
                     NAQGAAVEDEKRG"
     gene            2472..3704
                     /locus_tag="VGB76_00795"
     CDS             2472..3704
                     /locus_tag="VGB76_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY0097462.1"
                     /translation="MLREQVITPRTGRGGAQSGGGRGRAGDVAQQRPVKRGGAGGGQR
                     GGTATRKAAKGGAWGKALAWMPLIGKMLVVVCVLLVCFMAYRTAASSSFFALRTVDLS
                     GVSQGSEDQLKALVRRVGGAQGVWKADLDAISAELERQPWVRKAIVSRVLPSGLRVRI
                     TERTPRAVVRTSAGRFVWVDDDGVTVGTVAPTQNAPAFFMRGWDESGTESGRTENRRR
                     VEKYLELSREWEAAGVASRVSEVNLDDLRDVRAQLTGDDSQIEVRLGREDLTKRLRQS
                     LEALDEQRSTPRGAHITYIDMTQGKRAVIGFKAHALKQSDGDASNPGAHDGAASGDVA
                     DVAPDTNGANGTSRRAAETKKPLVQAANKPRPSVEKTAKSKEVKKAQQAGARENERPA
                     TGQTRGGMERPRRVGRSG"
     gene            3787..5052
                     /gene="ftsA"
                     /locus_tag="VGB76_00800"
     CDS             3787..5052
                     /gene="ftsA"
                     /locus_tag="VGB76_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY0097463.1"
                     /translation="MAKRGAHTVGLSIGTSRVRCIIGEPGDVSGALDIVGIGEAEARG
                     LRKGVIVNPENAVEAIKHAVEAAERMSGLEAEEVTINLAGSHIMGFNGQAIVAVSGRD
                     REITSDDVRRAIDSACAIQLPAGREIVDRLPQEFIVDDQDGINDPVGMIGARLAVKVH
                     IVTSPVTARQNAINAVNRAGLVVSDMVLEQLAAAEASLTEEDKEFGSALVDIGAETTG
                     LVIYQRGAVQHTAVFALGGSHFTNDIAFGLRTPIPEAEKIKRSVGFASSTGLSEMERG
                     ELVEVPSVGGRGPRQLSRQILCDILQPRAEEVLMHVADEIRESGWDRQLSSGVVLTGG
                     GSLLGGMVEVAEQVFDAPVRLGYPERDRFGGLIEDIQTPAWTAAAGLSLVAGRANAVE
                     ARSASGKRGATGGLTAFVSKFRNRFSSIF"
     gene            5196..6353
                     /gene="ftsZ"
                     /locus_tag="VGB76_00805"
     CDS             5196..6353
                     /gene="ftsZ"
                     /locus_tag="VGB76_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY0097464.1"
                     /translation="MTPESRQPGSGINIFIDDDPPITGARIKVIGVGGGGGNAVNRMI
                     EAGIEGIEFLVANTDLQALKRSRAPIKIQLGGKLTKGLGAGANPDVGRNAALEDTEKI
                     IEALEGADMVFVTTGLGGGTGTGAAPIIASLATELNALTVAVVTKPFHFEGKRRMQQG
                     DQGLRELRECVDTVITIPNERLLGNVTKDMSLTDAFKMADDVLRQAVQGISDLITVPG
                     LINLDFADVKSIMAGMGMALMGAGRASGEDRALKATQEAISSPLLEEASINGAKGVLI
                     NITGGLDLTLFEVNEASSIIREAADEDANIIFGAVIDESMRDEMKITVIATGFDKESA
                     DAGLVPNPNVMPAAQPRYIPRTDELPSRSTAAGQSRADDLDVPAFIRKKAD"
BASE COUNT         1274 a         1806 c         2217 g         1084 t
ORIGIN      
        1 ccgcgcggag aacgtgggcg atgcgcaggt ggtggttcgc tcgaccgccg tgcgcgacaa
       61 caacccggag ttgatcgagg cgcgcgagcg ttcgatcccc gtgatcgcgc gggctgagat
      121 gctggccgag ttgatgcgac tcaaacgcga tacggtcgcc gtcgcaggct cgcacgggaa
      181 gacgacgacc acttcgatga tcgggatggt cttgggcgcg gcgggactcg acccgacggt
      241 ggtggttggc ggcgtggtcg gtgccttcgg atcgaacgcg cgtctgggga agagcgatct
      301 gatggtggtg gaagcggacg aaagcgacag gtcgtttttg atgctcgcgc cgactttcac
      361 ggtcgtcacg aacatcgacc gcgaacacat ggactactac cgcgacatgc aggacatgca
      421 ggattgcttc atccgcttcg tcaacaaagt gccgttctac ggcgcgtccg tgttgtgtct
      481 cgacgatcct tacgtgcagg ctgtgatccc gcacgtcaaa cgccgccgca tcacttacgg
      541 cctgagcgcg caggcagacg tttcggcgca caacatcaag tttgatattc cgttcggttc
      601 gcgcttcacg gtctggcgcg ggcatgaagt gatgggcgaa gtggagatgc gcgtgccggg
      661 tttgcacaac gtctataacg cgctcgcggc catcgccatt agcttcgagt tggaagcgcc
      721 gtttgaaaag accgtggaag gcttgggtgc atacacgggc gtcaatcgcc gctttcagtt
      781 caagggcgaa gcgcagggcg tgctcgtcgt ggacgattac gggcatcacc cgacggagat
      841 tcgggcgacg ctcgcggcgg cgaagatcgg ttcttcgggc cggcgcatgg tcgtgctctt
      901 tcaaccgcac cgctacacgc ggacgcagga tcagatggaa gagttcgcgc gttccttcaa
      961 caacgccgac gtgcttctcg tcacagacat ctacgcggcg agcgaagacc cgattgaagg
     1021 catcacggcg gaggctctgg tcgaagcggt gaaacgctac ggccacaaga acactttcta
     1081 tgccggttct ctggagacag ccgtgaaagc tctcttgagc gaagcgcaca acggcgacat
     1141 ggtgatcacg ctcggcgcgg gcaacgtctt tcgcgcgggc gaagagttga tcgaactctt
     1201 gagcaaaaac ggccacgggc acggcaacgg caaatgaaag gagacgatga acgcggaatg
     1261 atgaatgatg aattaagaaa gcaaagaaga ctgcttttcg ttcctcattc cgccttcatc
     1321 atgcataaat ttttatcgtg acgacgatga cgcacagcac gatcagccca cgcgagcgcg
     1381 acgcggcgat tgaagagatc gcggcgcgtc tgggcgtgcg tgcggagcgc ggcaggcgtt
     1441 ttgccgagtt gacgagtttg cgtgtgggcg gggcgattga ttgggtcttt gcgccggaga
     1501 cggaagaggc ggcagcacag ttggccggtg agttggacgc ggcagggatc gcgtggcgcg
     1561 tgctcggcgc gggcagcaac gtcctcgccg acgacgaagc gcatcattac gtcgtcttga
     1621 gcatgcgcga ggtgaagggg acggcgttgt tcgagggcga gcgcgtgagc gtgagcgcgg
     1681 gttattcgct gccgcgcctg tgcgtggatg cggcgcggcg cggactcgca ggcatcgagg
     1741 gcttgggcgg catccccggc acggtcggcg gcgcgctctg gatgaacgcg ggcgcgttcg
     1801 ggcacgagat cggaaacgtg gtggagacgg tgcgtgtggc gcgcaggggg cgcgtggttg
     1861 aagtgcgcgg cagcgagatc gaatggaact accggcacac gtctttccgc gagggcgagt
     1921 tgttgttagg cgtgacgctg cgcctgcgtc ctgacgactc ggcgaagatc gtggaacgga
     1981 tggcggaagt gaagcggcag cggatggaga cgcagccgca cggcgcgcgt tccgccgggt
     2041 gcttcttcaa gaacccgccg gggagcggcc tcagcaccgg gcggatgatc gacgagatgg
     2101 gcatgaaagg cgagaagcgc ggcggcgcgg tcgtctcacc cgtccacgcc aacttcatcg
     2161 tgaccgaagg cgacgacgca cgcgcctccg acgccttcgc gctcgcagaa gagatcaggg
     2221 aacgcatacg acgcgagcgc ggcatcgagt tggaatatga agtggaacta tggcgcagcg
     2281 gcaacgcgcg ccaagcggtt gagagtaatg ctggcagaga cgaagcgggt tcgggcgaag
     2341 agtctgtgaa tgcgcagggc gcagcagttg aagatgaaaa gcgcggctga acattgaagg
     2401 acaagtcttt gacttgtcac tcgtcactcg tcacttgtta ctgtagttgg cacctgtcat
     2461 taggagtaaa gatgctgaga gagcaagtca tcacaccacg cacgggtcgc ggcggcgcac
     2521 agagcggcgg cggtcgtggc cgcgccggag atgtcgcaca gcagcgtccc gtcaagcgcg
     2581 gcggcgcggg tggcggtcag cgcggcggta cggccacgcg caaggctgcg aagggggggg
     2641 cgtggggcaa ggctctggcg tggatgccgc tcatcgggaa aatgctggtc gtcgtgtgcg
     2701 tcctgctggt ctgcttcatg gcttatcgca cggcggcgtc atcctctttc ttcgcgctgc
     2761 ggacggttga cctgagcggc gtgtcgcaag gttcggaaga ccaacttaag gctctggtgc
     2821 ggcgcgtggg cggcgcgcag ggcgtctgga aagccgacct cgacgcgatc agcgcggaac
     2881 tcgaacgcca gccctgggtg cgcaaagcga ttgtctcgcg cgtgctgcct tcgggcttgc
     2941 gcgtgcgcat cacggagcgc acgccgcgcg ccgtggtcag aacttcggcg gggcgtttcg
     3001 tctgggttga cgacgacgga gtgacggtcg gcacggttgc gccgacgcag aacgcgcccg
     3061 ccttcttcat gcgcggctgg gacgagtcgg gcacggaatc gggacgcacg gagaatcgcc
     3121 gtcgcgtgga gaaatatctg gaactgtcgc gcgagtggga agcggcgggc gtggcctcgc
     3181 gcgtgagcga agttaatctc gacgatctgc gcgacgtgcg cgcgcagttg acgggcgacg
     3241 attcgcagat cgaagtgcga ctcggtcgtg aggatttgac caagcggctc aggcaatctc
     3301 tggaagctct cgacgaacaa cgctcgacgc cgcgcggcgc gcacatcacc tacatcgaca
     3361 tgacgcaggg caagcgcgcc gtgatcggct tcaaagcgca cgcgctcaag cagagcgacg
     3421 gagacgcctc gaaccccggc gcgcacgacg gcgcggccag cggtgatgtc gctgatgtcg
     3481 cgcctgatac aaacggcgcg aacggcacga gccgtcgtgc ggcggaaacg aagaagccgt
     3541 tagttcaagc ggctaacaag ccgcgcccgt ccgtcgagaa gacggcgaag agtaaagagg
     3601 tcaagaaggc gcagcaggcg ggagcgcgtg agaacgagcg accggcgacg gggcagacgc
     3661 gcggagggat ggaacgcccg cgccgtgtgg ggcgcagcgg ttaaagcgcg gctgcggcgt
     3721 gggcggggcg gagaagaacg cgggcgaaag ttttcgcacc ctttgagaag gacgagagaa
     3781 accagcatgg cgaaacgcgg agcacacacc gtcggcttga gcatcggtac gagccgtgtg
     3841 aggtgcatca tcggcgagcc gggcgatgtg tcgggcgcgc tcgacatcgt cggcatcggc
     3901 gaagcggagg cacgcggcct gcgcaaaggc gtcatcgtca atcccgaaaa cgccgtcgag
     3961 gcgatcaagc acgcagtcga agccgccgag cgcatgagcg gactcgaagc cgaagaggtg
     4021 acgatcaacc tcgccggttc acacatcatg ggcttcaacg ggcaagccat cgtcgccgtc
     4081 tcagggcgcg atcgcgagat cacttcggac gacgtgcgcc gcgcgattga ttcggcctgc
     4141 gcgattcaac ttccggcggg acgcgagatc gtcgaccggc tcccgcagga gttcatcgtc
     4201 gacgatcagg acggcatcaa cgatccggtc ggcatgatcg gtgcgcgtct ggccgtcaaa
     4261 gtccatatcg tgacgagtcc cgtcacggcg cggcaaaacg ccatcaacgc cgtcaatcgc
     4321 gccggtctgg tcgtctccga catggtgctc gaacagctcg ccgccgccga agcgtctctg
     4381 acggaagagg acaaggagtt cggttcggcc ttagttgaca tcggcgcgga gacgaccggc
     4441 ctcgtcattt atcaacgcgg cgcggtgcag cacacggcgg tcttcgcgct cggcggttcg
     4501 cactttacga acgacatcgc gttcggactg cgcacgccga ttcccgaagc ggagaagatc
     4561 aagcgttcgg tcgggttcgc atcgagcacg ggtttgagcg agatggagcg cggcgaactc
     4621 gtcgaagtgc cttcggtcgg aggacgtggc cctcgccagt tgtcgcgcca gattttgtgc
     4681 gacatcctgc aaccgcgcgc cgaagaggtg ttgatgcacg tggccgacga gatacgcgaa
     4741 tcgggatggg acaggcagtt gtcgagcggc gtcgtcttga cgggcggcgg ctcgctcctc
     4801 ggcggaatgg tcgaagttgc ggagcaggtc ttcgatgcgc cggtgcgtct cggttatccc
     4861 gaacgcgacc gtttcggcgg cctcattgaa gacattcaaa ctccggcgtg gactgccgcc
     4921 gccggactct cgctcgtcgc cgggcgtgca aacgccgtcg aagcgcgctc tgccagcggc
     4981 aagcgcggag cgacgggtgg tttgactgct ttcgtttcga aatttcggaa tcgttttagt
     5041 agtattttct gacaagttgt tgtatgctgc cgcgccagcc ggcacaagat gttgtccgca
     5101 atatatgggt gtcaccggcg cgcacagccc aaaatagccg cacaggctct cctgcgagcg
     5161 gtgggcgttc aaagaagaga aaggacttat aagatatgac accggaaagc cgtcagccgg
     5221 gcagtggcat taatattttc atagacgatg atcctccgat caccggcgcg cgcattaaag
     5281 tgataggtgt tgggggcggc ggcggcaacg ccgtcaaccg catgatcgag gccggcatcg
     5341 agggcatcga gttcctcgtc gccaatacgg atttgcaagc gttgaaacgt tcgcgcgcgc
     5401 cgatcaagat tcaactcggc ggcaagttga ccaagggact cggcgcgggc gcaaaccccg
     5461 acgttggccg caacgccgcg ctcgaagata cggaaaagat tatcgaggct ctggaaggcg
     5521 cggacatggt cttcgtcacg acggggcttg gcggcggcac gggcacgggc gcagccccga
     5581 ttatcgcgag tctggcgacg gagttgaatg ctttgacggt cgccgtcgtc acaaagcctt
     5641 ttcactttga aggcaagcgg cgcatgcagc agggcgatca gggcttgagg gaacttcgtg
     5701 aatgcgtgga cactgtgatc acgattccga acgaacgcct gctcggcaac gtgacgaagg
     5761 acatgtctct gacggacgcc ttcaaaatgg ccgacgacgt gttgcgtcaa gccgtgcagg
     5821 gcatcagcga tctcatcacc gtacccggcc tgatcaatct ggacttcgcc gacgtgaaat
     5881 caatcatggc ggggatgggc atggccttga tgggcgcggg gcgtgcgagc ggcgaagacc
     5941 gtgcgctcaa agccacgcag gaagcgatct cttcgcccct gctggaagag gcatcgatta
     6001 acggcgcgaa gggcgtgctc atcaacatca cgggcggact cgatctcacg ctcttcgaag
     6061 tcaacgaagc ctcctcgatc atacgcgagg cggcggacga agacgccaac atcatcttcg
     6121 gcgcggtgat tgacgaatcg atgcgcgacg agatgaagat cacggtcatc gcgacagggt
     6181 tcgacaaaga gagcgcggac gccggactcg ttcccaaccc gaacgtgatg cccgccgcgc
     6241 agccgcgcta catcccgcgc acggacgagt tgccgtcgcg cagcaccgcc gccgggcaat
     6301 cgcgcgcaga cgatctcgac gtgcccgctt tcattcgcaa gaaagcagac taacatcagt
     6361 gacaagtgac aagtgacggg t
//
LOCUS       DASWNS010000029         7217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_364070, whole genome shotgun sequence.
ACCESSION   DASWNS010000029 DASWNS010000000
VERSION     DASWNS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364070"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VGB76_00810"
     CDS             complement(<1..1194)
                     /locus_tag="VGB76_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0097465.1"
                     /translation="MSTIAPPQRPEIKTELPGPKGQAIVEADAQYVTPSYPRPSFKLV
                     AERGEGVWVTDVDGNVFLDCNAGVAVCSTGHCHPEVVKAIQDQTAKLIHMCGTDYFYS
                     QMPDLARKLDEIVPVPSPTRTHFANSGTEAIETALKLAMHATGRQKFIAFFNSFHGRT
                     LGSLSLTSSKIAQRTGFKRSVLDVTHVPYPNAFRHPFTGEQTDEATVSRACLDWIEER
                     LFKTTTPPEEVAAIIVEVVQGEGGYVPAPRAFLEGLRRICDAHGIMLIVDEVQSGMGR
                     TGKMFASDHHDLKPDIICLAKGIASGLPMGACVARADLMDWKPGAHASTFGGNPVALV
                     AALKTIELLEGGLVSNSAEVGAYLKAGLEKLKEKHDCIGDVRGLGMMIGVEFVEDRAS
                     MKPAPE"
     gene            complement(1495..2895)
                     /gene="cysS"
                     /locus_tag="VGB76_00815"
     CDS             complement(1495..2895)
                     /gene="cysS"
                     /locus_tag="VGB76_00815"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY0097466.1"
                     /translation="MLKFQNTLSGQLEEFRPMREGEVRMYNCGPTVWDFAHIGNFRTS
                     IFVDILRRYLIFKGYKVLHVTNITDVDDRIIQMAAQKNVTLDEYTAPYLAAFWEDFDA
                     LGCQRPEIAPRATHHIPEMEEIVKRLAERGCAYETEDSYYFRITSFPEYGKLSKINFA
                     GNIAGASERVDTDKYEKEDARDFVLWKAQKSADEPAWQTALGNGRPGWHIECSAMSMK
                     YLGETFDIHCGGVDLIFPHHENEIAQSEGATGKPFVNYWLHGEHLKINGESMSKSKGN
                     YYTFRDLTRQGFTPAAIRYALFSAPYRKQINFTLDGLRGHEKTVASLQDFRARLEEAR
                     TEAGSNPAMRAVAARALEEFTNGLDDDLNTSVALAAVHNLSREVNTALDNHALRADDR
                     SDLLALIARFDSVLNIFGHTEREMLDAEIQALIDERQQARHRRDFARGDEIRDQLAAR
                     GIILEDTRDGVRWKRR"
     gene            complement(2981..3490)
                     /locus_tag="VGB76_00820"
     CDS             complement(2981..3490)
                     /locus_tag="VGB76_00820"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0097467.1"
                     /translation="MPNTLLRIAVALFAFGLGVSATTLWIAFRTPEVKSMETEPYRKR
                     ECSARPRMPLPDEHFLPPLAEPPPPPRPLLPRAPISGGVLDGKAVSKPQPAYPSVALA
                     GRAAGAVYVQVTVDESGQVISAKAIGGHPLLQSAATEAANRARFSPTRLEGQPVKVSG
                     VLSYNFVLP"
     gene            complement(3642..4793)
                     /gene="thiL"
                     /locus_tag="VGB76_00825"
     CDS             complement(3642..4793)
                     /gene="thiL"
                     /locus_tag="VGB76_00825"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY0097468.1"
                     /translation="MKDEVKRKARPVVPPRASPVVARSSTGQLPNGEFDFISRIKRHA
                     EASRLNKTGGLVQGIGDDAAVLTGRGVGRSGRDSVITADLLVEDIDFRRAYLPHDIGY
                     KALAVSLSDIAAMGARPRWTLLSLGLPQDVWESPFAAGISEGFLFAAREFGVTLIGGD
                     VSRTPERIVIDSIVIGETRRGGAVLRSGARAGDQIYVTGTLGGAAAGLKLMERARSLR
                     TPGARTRLLSGFEELTRRQTHPTPRVACGALLGERRLVSAMIDLSDGLSSDLAHLCER
                     SGVGAFIEASRLPVAPLLGQPGASASHSLARALKEDALDFALHGGEDFELLFTVRPRA
                     AARLPKRLGGVRLTRIGEVREGVEGIRISRGGRVEILQPSGFDHFKQVR"
     gene            complement(4819..5991)
                     /locus_tag="VGB76_00830"
     CDS             complement(4819..5991)
                     /locus_tag="VGB76_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0097469.1"
                     /translation="MVSTAPSVYRLAARLEAVGFSDIVKIRNRIMELRAAGATVYQFE
                     GGEPFLNTPEHVKEAMTRALAEDRTRYAPSSGIQELREAIAQKLRTRNHMPVEAKDVI
                     VQNGGMQGLFGAFQSVVNPGEEVLLFSPYWTPVKDLIAGCEARSVLVSTEEARREGFA
                     ATLARHTTAKTRAIYYNTPQNPTGVVFTREEAEAVAAFARERNLVVIADEAYEDLVYE
                     GEHVSIASLEGMWERTLTVYTLSKSYSMTGWRIGYVVAAEPWMTGLRKTTLYSTNGVS
                     TPTQWAALAAINTPPDFFAATRAEYRERRDLMIDGLNEIGLKCAPPAGAFYAFADVTR
                     IHADSRTAAEILLDRAQVATVPGIVFGEHGEGHLRFSFSTSLDAIRDGLDSMRRKL"
     gene            6428..7042
                     /locus_tag="VGB76_00835"
     CDS             6428..7042
                     /locus_tag="VGB76_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097470.1"
                     /translation="MNLQNKILPVALVAALLGGTVGAFVMRPKTNELNTVASSNLLPA
                     AKDVNSQAASLRYNETLPANNNETPATLNVANTSSDEECYRAGFTEGFAAAREDKNLR
                     SSNLTNSATRSTERVVYRDAPVRRTRVARNTRASRQAYYDYEPRKRSFWSKHRDKLTV
                     AMGAGGGALVGGLIGGKKGAAIGALGGGAGSALYTYKIRNRNRR"
BASE COUNT         1332 a         2084 c         2384 g         1417 t
ORIGIN      
        1 ctccggcgca ggcttcatcg aagcgcggtc ttccacgaac tcgacgccga tcatcatgcc
       61 gagtccacgc acgtcgccga tgcaatcgtg cttctctttc aacttctcca gaccggcttt
      121 caggtacgcg ccgacttccg cgctgttcga gacgaggccg ccttcgagca gttcgatggt
      181 cttcaaggcg gcgacgagcg cgacggggtt gccgccgaaa gtcgaagcgt gcgcgcccgg
      241 tttccagtcc atcaaatcgg cgcgcgccac gcacgctccc atcggcagcc ccgacgcgat
      301 gcctttcgcg aggcagatga tgtccggctt caagtcgtga tgatcgctgg cgaacatctt
      361 gcccgtgcgc cccatgccgg attgcacctc gtcaacgatg agcatgatgc cgtgcgcgtc
      421 gcagatgcgg cgcaagcctt cgaggaacgc gcgcggcgcg ggcacgtaac caccctcgcc
      481 ctgcaccact tcgacgatga tcgcggccac ttcttcgggc ggcgtggtcg tcttgaagag
      541 acgttcttcg atccaatcga gacaggcgcg gcttacagtc gcttcgtccg tctgctcgcc
      601 cgtgaaggga tggcggaagg cgttcgggta gggcacgtgc gtcacgtcga ggacgctgcg
      661 cttgaagcct gtgcgttgcg cgatcttcga cgaagtgagc gacaggctcc caagcgtgcg
      721 cccgtgaaaa ctgttgaaga aggcgatgaa cttctgtcgc ccggtggcgt gcatggcgag
      781 cttgagcgcg gtttcgatgg cctccgtgcc gctgttggca aagtgcgtgc gggtgggcga
      841 ggggacgggc acgatctcgt cgagcttgcg cgcgaggtcg ggcatctggc tgtagaaata
      901 gtccgtgccg cacatgtgta tgagcttggc ggtttgatcc tgaatcgctt tgacgacttc
      961 aggatggcaa tgacccgtcg agcagacggc gactccggcg ttgcagtcga gaaagacgtt
     1021 gccgtcaacg tccgtgaccc agacgccttc gccgcgttcg gcgacgagtt tgaaagaggg
     1081 gcgcgggtaa gaaggcgtga cgtattgcgc gtcggcttcg acgatggcct gaccctttgg
     1141 gccgggcagt tcggttttga tctccggcct ctgcggcggg gcgatggttg acatggaaat
     1201 ttctccttca gtgaaaagaa cagatatgaa tgacaactct cgattttcaa agttggaatg
     1261 agagagttgc gacgtgcggg cgcgttgaag gaataatgtg cgaggctagc cggcgaagat
     1321 gttcgcgcgg aggtcgagcg gtcgaagatt ggaaagcggt gaatcaaaca tgggaaaacg
     1381 tcttttgcct tttgtccttg gcccgtcgcc ggtggttcaa gaccgcgagc gaaggtggat
     1441 gattatttag cacaaacgac ggacaactgc ctaccgacgg cgaaggattc tgttttagcg
     1501 tcgcttccag cgcacgccgt cgcgcgtgtc ttcgaggatg atgccgcgcg cggcgagttg
     1561 gtcgcggatt tcgtcgccgc gtgcgaagtc gcggcggtgt cgcgcctgct ggcgttcgtc
     1621 gatgagagcc tgaatctcgg cgtcgagcat ctcgcgctcg gtgtggccga agatgttgag
     1681 cacggaatcg aagcgtgcga tgagcgcgag cagatcgctt ctgtcgtcgg cgcgcagagc
     1741 gtggttgtcg agcgcggtgt tcacttcgcg cgagagatta tggacggcgg ccagcgcgac
     1801 cgaggtgttg agatcgtcgt ccagaccgtt ggtgaactct tcgagcgcgc gggcggcgac
     1861 ggcgcgcatg gcgggattgc tgcccgcttc ggtgcgcgct tcctcaaggc gggcgcgaaa
     1921 atcctgtagg ctcgccaccg tcttctcgtg gccgcgcagg ccgtcgagcg tgaagttgat
     1981 ctgcttgcga tagggcgcgg agaagagcgc gtagcggatg gcggctggcg taaagccctg
     2041 ccgcgtgaga tcgcggaagg tgtaatagtt gcccttcgat ttcgacatcg actcgccgtt
     2101 gatcttcaag tgctcgccgt gcagccagta gttgacgaag ggctttccgg tcgccccttc
     2161 cgattgcgcg atctcgtttt cgtgatgcgg gaagatgaga tcgacgccgc cgcagtggat
     2221 gtcgaacgtc tcgccaagat acttcatgga catcgccgag cactcgatgt gccagccggg
     2281 acgaccgttg ccgagcgcgg tttgccacgc gggttcgtcc gcagacttct gcgccttcca
     2341 gagcacgaaa tcgcgcgcgt cctctttctc atacttgtcc gtgtccacgc gttcgctcgc
     2401 gcccgcgatg ttgcccgcga agttgatctt cgagagcttg ccgtattcgg gaaaagaggt
     2461 gatgcgaaaa tagtaggagt cttcggtttc gtaggcgcag ccgcgctccg cgagacgttt
     2521 gacgatctct tccatctccg ggatgtggtg cgtggcgcgc ggggcgatct cgggtcgctg
     2581 gcagccgagc gcgtcgaagt cttcccagaa ggccgcgagg tagggagcgg tgtattcgtc
     2641 gagcgtgacg ttcttttgcg cggccatctg gatgatgcgg tcgtccacgt ccgtgatgtt
     2701 tgtgacgtgc agcaccttgt agcctttgaa gatcaggtaa cggcgcagga tgtcaacgaa
     2761 gatggacgtg cggaagttgc cgatgtgcgc gaagtcccag accgtgggac cgcagttata
     2821 catgcgtacc tcgccctcgc gcatggggcg aaattcttcg agttgacctg agagagtgtt
     2881 ttgaaatttc aacatcttag ttcgaacgca cggagacgga cgctgcgccc gcgccgccga
     2941 cgaatcggac gcgccatcag agtgggcttg ttccgcgcgt ttatggaagc acgaagttgt
     3001 agctgagcac gccggagact ttgacgggct gaccttcgag ccgcgtgggc gagaagcgcg
     3061 ctcgattggc cgcttcggtc gccgcgcttt gcagcaacgg atgaccgccg atggctttgg
     3121 cggagatcac ctgaccgctt tcgtcaaccg tcacctgcac gtagaccgcg cctgcggcac
     3181 gtccggcgag cgcgacggac gggtaggcgg gctgcggctt gctgacagct ttgccgtcga
     3241 gcacgccgcc ggagatggga gcgcgcggca agagcggcct cggaggcgga ggcggttcag
     3301 ccaaaggcgg caggaagtgt tcgtccggca gcggcattct ggggcgtgcg ctgcactctc
     3361 tctttctgta gggttcagtc tccattgatt ttacttccgg cgtgcggaag gcgatccaga
     3421 gcgtggtggc gctgacgccg aggccgaaag cgaagagggc aactgcgatt cggagcaatg
     3481 tgtttggcat aactttactt cctttgctga acgagagcta gagggacgac cccgccgtcc
     3541 ggtctgaaat aatcgtcctt cagcgagagt ctaggcaagt ctaggcggct agtcaaattt
     3601 taggttctac cggagggaag tacgaagcag cggcgacagg atcagcgcac ttgtttgaag
     3661 tggtcgaagc cggaaggttg caggatttct acccgcccgc cgcgtgagat tcggatgcct
     3721 tcgacgccct ctctcacctc gccgatgcgt gtgagccgca cgccgccgag ccgcttcgga
     3781 agtcgcgcgg cagcgcgcgg gcgcacggta aacagcaact cgaaatcctc gcccccgtgc
     3841 agtgcgaagt cgagcgcgtc ctcttttaaa gcgcgtgcga gactgtgaga ggcagacgcg
     3901 ccgggctgtc cgagcagggg cgcgacgggg aggcgcgatg cttcgatgaa cgcgccgacg
     3961 ccgcttcgtt cgcagagatg cgcgaggtcg gatgagagtc cgtcgctcag atcaatcatg
     4021 gcagagacca gacgccgctc acctaagagc gcgccgcagg cgacgcgggg cgtgggatgc
     4081 gtctgccgcc gcgtgagttc ttcaaagccg gagaggagac gcgtgcgtgc gccgggcgtg
     4141 cgcaggctgc gggcgcgttc cattaacttc aagcccgccg ccgcgccgcc gagcgtgccc
     4201 gtcacgtaga tttgatcgcc cgcacgcgcg cccgaacgca acaccgcgcc cccgcgccgc
     4261 gtctcgccga tcacgattga atcgatcacg atgcgttcgg gcgtgcgcga cacgtcgccc
     4321 ccgatgagcg tcacgccgaa ctcacgcgcg gcaaacagga agccttctga aatgcctgcg
     4381 gcaaacggcg actcccaaac gtcctgcggc aggccgagcg agagaagcgt ccagcgaggt
     4441 cgtgcgccca tcgcggcgat gtccgagagc gagacagcga gagccttgta gccgatgtcg
     4501 tgcggaaggt atgcgcggcg aaagtctatg tcttcgacga ggaggtcggc ggtgatcacg
     4561 gagtcgcgcc cgctgcgtcc cacgccgcgc ccggtgagca cggcggcgtc gtcgccgatg
     4621 ccttgaacga ggccgcccgt cttatttaga cgcgacgctt ccgcgtgtcg cttgatgcgc
     4681 gagatgaaat cgaactcgcc gttcgggagt tggccggtcg atgaacgcgc gacgacgggt
     4741 gatgcgcgag gcgggacgac cggacgagct ttccgcttta cttcatcctt catctctccg
     4801 ctctccgccc tcgacgtttc acagcttccg gcgcatcgaa tcgagtccgt cgcggatggc
     4861 gtcgagcgag gtggagaagg agaaacgcag gtggccttcg ccgtgttcgc cgaagacgat
     4921 gccggggacg gtggcgactt gcgcgcggtc gagcagtatc tcggcggcgg tgcgactgtc
     4981 ggcgtggatg cgcgtgacat ccgcgaaagc atagaaggct ccggcgggcg gcgcacactt
     5041 gaggccgatc tcgttcaagc cgtcgatcat caggtcgcgg cgttctcgat actcggcgcg
     5101 cgtcgccgca aaaaagtcgg gcggggtatt gatggcggcg agggcagccc attgggtcgg
     5161 cgtcgagacg ccattggttg aatagagcgt ggtcttgcgc aggccggtca tccacggttc
     5221 ggcggccacg acgtagccga tgcgccagcc cgtcatcgaa taagacttcg agagcgtata
     5281 gacggtgagc gtgcgttccc acatcccttc gagcgaggcg atagagacgt gttcgccttc
     5341 atagaccagg tcttcgtaag cctcgtcggc gatgacgacg aggttgcgtt cgcgtgcgaa
     5401 ggcggcgacg gcctcggcct cctcgcgcgt gaagaccacg cccgtcggat tttgcggcgt
     5461 gttgtaatag atggcgcgcg tcttggccgt cgtgtggcgc gcgagcgtcg cggcaaaacc
     5521 ttcccggcgc gcttcctctg tcgaaaccag cacggagcgc gcttcgcagc ccgcgatcaa
     5581 atctttaacg ggcgtccagt agggagagaa gagcagcact tcttcgccgg gattgacgac
     5641 cgactggaac gcgccgaaca aaccctgcat gccgccgttc tgcacgatca cgtccttcgc
     5701 ttcgacgggc atgtggttgc gcgtacgcaa tttttgggcg atggcttcgc gcaattcctg
     5761 tatgccggag gagggcgcgt agcgcgtgcg atcttcggcg agggcgcgcg tcatcgcttc
     5821 tttgacgtgt tcgggcgtgt tgagaaacgg ttcgccgcct tcaaattgat agacggttgc
     5881 gcccgccgcg cgcaactcca tgatgcggtt gcgaatcttg acgatgtcgg aaaacccgac
     5941 cgcctccaga cgcgcagcga gacgatagac ggacggagcg gtagacacca tggcgacgaa
     6001 cctccaaatt tttcaggcgt ggaagagaag cgggattata gcttgccccc gccgcgtgcg
     6061 tccaactcgc ccgcccttta gagaagccgt ttaaagaaac acgcaggcgt cgagcttgtc
     6121 gttgagcttg tcattaagct tgccgttgag cagggtattg gaggaggtcg ttgagcattt
     6181 cgtgtctcga ccgataccat atcgtgcacg attgcgattc cggcgcgcac tcggtcgagc
     6241 gtcaatatta cattttcgtt gcaaagagaa aattcagcct ttgaatgtct gaaaaaacga
     6301 gtagttgagg ttaaactgtc ggcgactctc cctctgacac gacttgcgga acgtagcttg
     6361 ctcatttatc gagcgggtta atcgaactca cctgaaccat ttggcgaatt gaaggagaag
     6421 ctgaccaatg aacttacaga ataagatttt accagtcgca ctcgtggccg cgcttttggg
     6481 tggcactgtc ggcgcgttcg tgatgcgccc caagacgaac gaactcaaca cggtcgcctc
     6541 atcgaatttg ttaccggctg cgaaggatgt gaattcgcag gccgcgtcgt tgcgttataa
     6601 cgagacgctc ccggcgaaca ataatgagac gcccgcgact ttaaacgtcg ccaacacgtc
     6661 gagcgacgag gaatgctatc gcgccggttt caccgaagga ttcgcagcgg cgcgtgaaga
     6721 caagaacctc cgcagcagca acctgacgaa ctccgcgacc cgctcgaccg agcgcgtcgt
     6781 ctaccgcgac gctccggttc gccgcacacg tgtggcgcgc aacacgcgtg cttcgcgtca
     6841 ggcttactac gattatgaac cgcgcaagcg ttcgttctgg tcgaagcacc gcgataaatt
     6901 gacggtggcg atgggcgcgg gcggcggcgc gttggttggc ggcctcatcg gcggcaagaa
     6961 gggcgcggct atcggcgcac tcggcggcgg tgcaggctcg gccttgtaca cgtacaaaat
     7021 ccgcaaccgc aatcgtcgct aattcaagcc gggacggtgt gagatggatc gtggctcgaa
     7081 gcagacggcg cgacgcgcaa cttcagcttt cctgatcgct ctggcagtaa cgttatgggc
     7141 agttcccaac gagggacctg cccattcttt tgtccacggg cgcgagcgaa tgacgaccca
     7201 cgcccgcgcc gcaaacg
//
LOCUS       DASWNS010000030         8442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_312067, whole genome shotgun sequence.
ACCESSION   DASWNS010000030 DASWNS010000000
VERSION     DASWNS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312067"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VGB76_00840"
     CDS             complement(<1..1118)
                     /locus_tag="VGB76_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097471.1"
                     /translation="MNSASLRAVLSRLTLAAVLALLASPPAVSQKNCGKEKNPYTGMI
                     ISINPSHPAGKLKIRLNGRAAPFNAEPWMTVRRGALLELAEGASATIMCGDQSQHSLK
                     SGANPVPCSDKSFVNRNGGCIAPARGGDNFDGSFPVIVAPRRTLLLTRRPTFRWTPVS
                     KSAAHAAASDPEITYTVSLFGAGMELIWLEKTSANVLPYPANRPELKAGDYQLVVVAG
                     NFGSSEKERMPDTGFTVLEDCQPQASRAAACKAREVRAEVNAIRRLRLPPDAEKLMLA
                     YVYMDRELYAEAIESLNAIVAPKSLPVVRLLGDLYLLTGLNREAERSYLDALSLPRMA
                     DEPEDKALSLSALAEIYEARGARSDAGARWDEAIKVYER"
     gene            complement(1111..2382)
                     /locus_tag="VGB76_00845"
     CDS             complement(1111..2382)
                     /locus_tag="VGB76_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0097472.1"
                     /translation="MGGIEDSISRYERWLDNLPSPASNEYEQHVWEILLARDEVANAL
                     AESQGVTASTFGRVAELDRRLKDKASVIRNTVGANKLLNWREVKSPPERSWWWFLDER
                     VVKEEAEAEEKRLKYDAVWILLTFFFVTVSLSLLTEITRRFFSGEGEWVSILGSVVQV
                     PLNVLSALLGLLTVGTITEAGRHGVQGVLQRAGLFKQAGVMRRFAIALALFCLVFPLR
                     LTLPLFAHYYNDLGGSQARAGHISKAIESYERAITLKPEYVEANYNLADAYERLPNCQ
                     KAISHYEAAIKLDHALTHAQNNLARLYLKCGNEAQTLTALAILDQQLRQHPPPDDDVR
                     YSLLKNQGWARYKLKNYAAAESDLRQAKAIRADRGSAHCLLAYTLEAQGRADEAIDEW
                     YDCIAYSVDVDVEAVWVDEAREKYGRKKSYE"
     gene            complement(2517..3467)
                     /locus_tag="VGB76_00850"
     CDS             complement(2517..3467)
                     /locus_tag="VGB76_00850"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097473.1"
                     /translation="MILIISHLADPHATTVLDHLARAGSEALLFDNARFPREIRLDIT
                     HATGERSAANVSIDGTRRDMSGVRCVWWRRPQPFVIPEEVSGSEDRNFAYGECHAAVV
                     GLWSCLDAHWVNNPERDEVAARKAYQLKVAVGLGFRVPRTLITNDPASAATFIEGEGA
                     AGTIYKAFSATERAWRETRLLRPEELAQLDAVRFAPVIFQEHIRADIDLRITIIGDEV
                     FPAAIRSGETDYHVDFRMTMHEAPIEAHELPPEIVSKLRSFMNALGLVYGAIDMRLTP
                     EGEYVFLEINPAGQWLFVEERTGQPITAALAAYLLAADRA"
     gene            complement(3513..4946)
                     /locus_tag="VGB76_00855"
     CDS             complement(3513..4946)
                     /locus_tag="VGB76_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0097474.1"
                     /translation="MSKENSGSDNPRRPAETGSRSIEESVRSSEDRADDPLFVPRIEP
                     LELTSERLDGGVGDDYAALLETICGANDDSQPVEQYNGALGVTTAFVAAHQSAACQVQ
                     WNSNLASIYTNPGDVSGARWGSGTMITNDLFLTCGHLFDQTGGGWQRPRQNGTSNIIS
                     PQEIARNMHLNFNFQVDPAGNLRPEQSFAIVELIEYRLGGLDMAICRIAGNPGATFGR
                     GVVSTTDAVVPEMLCIIGHPAGRPKRIEAGPTTRLAGNLIHYGDIDTLGGNSGSGILR
                     ARDGRLVGIHTNGGCTRDSPGDGGTNFGQRIAAVIAASPTLRALVAPKNKLADDPVTV
                     KFRDDVGNKRKVLDDIAVTLKFRDDVVAKRKVLDDLTLSKPPALEGKPPGADNPGNIG
                     DRPRPFGESPGAGGQPARPFILGTPHHSNAWGGEGQQAEVLGQIEHALMQLEQAMAAG
                     EAELMQLDAEYRQLLAEYEAAGGNLGG"
     gene            complement(5627..7849)
                     /locus_tag="VGB76_00860"
     CDS             complement(5627..7849)
                     /locus_tag="VGB76_00860"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0097475.1"
                     /translation="MSSTNQSGHYEKQHRGTQAAYEAYFAGMDASMQQKVALTTAHFP
                     VRGRIADMGSGSGRGTYDLACLYGGLELVGVDINPVSVERAAQQFRRANLSYVVGDIS
                     GMAFPAESLDGILDSSVLHHVTSFNNFDVNRVLDTLDNQVAQLKTGGVIIIRDFVIPD
                     GAEQVYLDLPGKGGLETGDVRELSAAALFELFARNWRSSVNRNAPVPYTRMESTRRGF
                     VRYEVALRAAAEFVLRKDYRADWETEILEEYTYLTQSQFEAAFRSRGLRIVTSRPLWN
                     PWIVQNRFEGKFYLSDTDEAPLPFPPTNYLIVGEKVRAGAGVELFEKERRAISSPNFL
                     SLRGYRHEATGHVMELVERPHQTLDVVPWFESRGQVFVFAKKDFPRPVVNACADQPRL
                     NSTTNSGYITEPISAIVNSEGDEEEEVCRILRERGGFEAEDIINVGGSFSYYTSPGGV
                     NELVISRLVEVRPGQTDFTFPNYTPFKDAGSVRELDATQVLRACHVGGLFDARLEINI
                     YRLLQELGRNYGPWIGAPISLSVQPLTLALSSPAQVITPQAHAAFSQPAPLSQPRFLS
                     LYEGSFAERDREGRILAGARFEYVRPEHLSHNTLVVLPVIRTEQGVFVGVEHRDLPAV
                     QSFSGSSRITVTPAWRLPSTIRRHSEIPAFLEEALARDFNLRVDRAWELGGSYFPTSG
                     VTPEVVYPFAAEVKPGKNPDSALQFIPLGEITAKPYLLQDAHLLIAAYRLAHALGNLP
                     "
     gene            complement(7877..>8442)
                     /locus_tag="VGB76_00865"
     CDS             complement(7877..>8442)
                     /locus_tag="VGB76_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097476.1"
                     /translation="QREHIFAVEFIELKGDILLGIKPVANVMIKSTRMFQGLAIQNLL
                     GAYYASIFPSPGVCHQVRLSELDRYQILDARLSRNVLMVVGAQGGRYDKLIFRFAKDF
                     SDYDSRLVPDVSSTDLNFTVLDSGVVLHLNDEGALEVFSRLRGASGIRTFQDPSVRGD
                     DRLFHTGGQALTARGSKLYKFTMLGQP"
BASE COUNT         1618 a         2533 c         2558 g         1733 t
ORIGIN      
        1 cgctcataaa cctttatcgc ctcgtcccat ctcgccccgg catcgctgcg cgcgccgcgc
       61 gcctcgtaga tttcggcgag cgcagagaga gagagggctt tatcctccgg ctcgtcggcc
      121 attcgtggca ggctcagggc gtcgaggtaa gaacgctcgg cttcgcggtt cagccccgtc
      181 aacaggtaga ggtcgccgag caggcggacc accggaagac ttttcggggc gacgattgcg
      241 ttcagcgact cgatggcttc cgcgtagagt tctcgatcca tgtagacgta ggcgagcatc
      301 aatttctccg cgtccggcgg gaggcgcaga cggcgaatcg cattcacttc cgcacgcacc
      361 tcccgcgctt tgcaagcggc ggcgcgactc gcctgcggct ggcagtcttc cagtaccgtg
      421 aagcctgtgt ccggcatgcg ctccttctca gagctgccga agttaccggc gacaactacc
      481 aactggtagt cgcccgcttt tagctccgga cggttggccg ggtatggcag aacgttcgcg
      541 ctcgtcttct ccagccagat caactccatc cccgcgccga agagactcac ggtgtaggtt
      601 atctccgggt cggaggcggc agcgtgcgcg gcggacttgg acacgggcgt ccaacggaaa
      661 gtcggcctcc tcgtcaacag gagcgtccgg cgcggcgcga cgatgacggg gaaggagccg
      721 tcgaagttgt cgcctcccct cgccggtgcg atgcagccgc cgttgcgatt gacgaaagat
      781 ttatccgagc aggggacggg gttcgcccct gacttcaagc tgtgctggct ttgatcgccg
      841 cacatgatcg tcgcgctcgc gccttcggcc agttccaaca acgcgccgcg cctgaccgtc
      901 atccacggct ccgcattgaa aggtgctgcc ctgccgttca acctgatctt gagtttccct
      961 gccgggtgcg acgggttgat agagatgatc atgcccgtgt aaggattttt ttccttcccg
     1021 caattcttct ggctcacggc gggcggtgac gccaacagcg cgaggacggc ggccagcgtc
     1081 agcctgctta agacggcgcg tagcgaggca ctattcatag ctcttcttcc tcccgtattt
     1141 ctccctcgcc tcatcaaccc agacggcctc cacgtctaca tctacggaat aggcgatgca
     1201 gtcgtaccac tcatcaatgg cttcatccgc acgtccctgc gcctcaagcg tgtaggccag
     1261 caggcagtga gccgagccac ggtctgcacg gatcgctttc gcttgccgga ggtcgctttc
     1321 ggcagccgcg tagttcttca atttgtatct cgcccagcct tgattcttca gcagtgaata
     1381 acgcacgtcg tcgtcgggcg gcgggtgctg ccggagctgt tgatccagta tcgccaacgc
     1441 cgtcagggtc tgcgcttcgt ttccgcattt cagatacagc cgcgcgaggt tgttctgcgc
     1501 gtgcgtgaga gcatggtcca acttgatcgc cgcctcgtag tgcgagatgg ccttctggca
     1561 gttgggtaaa cgctcgtagg cgtccgcaag gttgtagttc gcctccacgt attcgggctt
     1621 gagcgtgata gcccgttcgt agctctcgat ggccttcgaa atatgccccg ctcgcgcctg
     1681 actcccgccg aggtcgttgt aataatgtgc gaagagagga agcgtcaggc gtaagggaaa
     1741 gacgaggcag aagagagcga gcgcgatggc gaagcgtctc atcacaccgg cctgcttaaa
     1801 tagccctgcg cgttgcagaa ccccctgcac gccgtgtcgt cccgcctccg tgatcgttcc
     1861 cacggtcagg agtcccagca gcgcgctcaa cacgttgagc ggcacctgta cgacggagcc
     1921 gaggatgctc acccactccc cttcaccgga gaagaaacgg cgcgtgatct ccgtgaggag
     1981 actgagcgag acggtgacga agaaaaacgt caggagaatc cagacggcgt catacttcag
     2041 acgcttctct tccgcctccg cttcttcctt caccacgcgc tcgtccagaa accaccacca
     2101 agacctctcc ggcggcgact tcacctcgcg ccagttcaag agcttattcg cgccgaccgt
     2161 gttcctgatg acgctcgcct tatccttcaa cctgcgatcc agttcagcca cgcgcccgaa
     2221 agtcgaggcc gtgacgccct gactttccgc taacgcgttc gccacctcat cgcgcgccag
     2281 cagtatctcc cagacatgct gctcatactc gttgctcgca ggcgacggca aattgtcgag
     2341 ccaccgttcg tagcgtgata tcgaatcttc gatacctccc ataaagtttc tccgggtgct
     2401 tgcgctcgta ttgtgtcgcg atcaactttc ttatcgcgac ccgaagagat gaggcgcggg
     2461 cttgcagttg accatttcga cagtatgtag gccgttcaaa gatacgcgcc cgccggttaa
     2521 gcgcggtcag ccgctaagag atacgccgcc aacgccgccg tgatgggctg gccggtgcgc
     2581 tcttcaacga acaaccattg accggcgggg ttgatctcaa gaaagacgta ctccccttcc
     2641 ggcgtgaggc gcatgtctat cgcgccatag acgaggccga gcgcattcat gaacgagcgt
     2701 agcttcgaga ctatctcagg gggcagttca tgcgcctcga tgggggcttc gtgcatggtc
     2761 atgcgaaagt ccacgtgata gtccgtctcg ccggagcgga tcgcggctgg gaagacctcg
     2821 tcgccgatga tggtgatacg caggtctatg tcggcgcgga tatgctcctg aaagatgacg
     2881 ggagcaaacc gtaccgcgtc gagttgcgcc agttcctccg gcctcaacag acgcgtttca
     2941 cgccacgccc gttcggtcgc ggaaaaagct ttatagatgg tgcccgccgc tccttcgccc
     3001 tcgatgaacg tcgcggcact ggcgggatcg ttggtgatca gcgttcgggg cactctgaat
     3061 cccagcccga ccgcgacctt cagttgatac gctttgcgtg cggccacctc gtcgcgttcc
     3121 gggttattga cccagtgcgc atcgaggcaa gaccacagcc ccacgacggc ggcatggcat
     3181 tcaccgtaag cgaagttgcg atcctcgctg cccgacactt cctccggtat gacgaagggc
     3241 tgcgggcgtc gccaccagac gcagcgcacg ccggacatat cgcgccgggt tccgtctatc
     3301 gacacgttgg ccgccgaacg ttctccggtc gcgtgcgtaa tatcgagtcg tatctcacgc
     3361 gggaagcgtg cgttgtcgaa gagcaacgcc tcgcttcccg cgcgagcgag gtgatcgagc
     3421 acagtggtcg cgtgcgggtc ggccagatgt gaaatgatca agatcataac taagatttac
     3481 gcagcgcgac gacggccagc gcagccggac catcaacctc cgaggttgcc gcccgccgcc
     3541 tcatactcgg cgagcaactg ccgatactcc gcgtcgagtt gcatgagttc ggcctcgccc
     3601 gccgccatcg cctgctcaag ttgcatcaga gcatgctcta tctggccgag cacttctgcc
     3661 tgctggcctt cgccgcccca ggcattggaa tgatgcggcg tgccgaggat aaagggacgg
     3721 gctggctggc cgcctgcgcc cggagactcg ccgaaaggtc gcggcctgtc gccgatgttg
     3781 ccggggttgt ccgcacccgg cggtttgcct tccaaggcgg gaggcttgga gagggtcagg
     3841 tcgtccaaca cctttcgttt ggcgacaacg tcgtcgcgga atttcagcgt caccgcaata
     3901 tcatcgagca ccttccgttt gttgccgaca tcatcgcgga acttaaccgt gacggggtcg
     3961 tccgccagtt tattcttcgg ggcgaccaga gcgcggagcg tcggcgaggc ggcgatcacg
     4021 gcagcaatcc gctggccgaa atttgtgccg ccgtcgccgg ggctgtcgcg cgtgcagccg
     4081 ccgttcgtgt ggataccgac gagccgccca tctctggcgc gcaggatgcc ggagccggaa
     4141 ttgccgccga gcgtgtcgat gtcgccgtaa tgaatgagat tgcccgcgag ccgcgtggtt
     4201 ggtcccgctt cgattcgctt cggacgcccg gcgggatgac cgatgatgca gagcatctcc
     4261 ggaacgacgg catcggtggt tgacaccacg cctcgtccga aggtcgcacc gggattgcct
     4321 gcgatgcggc agatagccat gtcgagtccg ccgagccggt actcgatcag ttcgacgata
     4381 gcgaaactct gttcgggtcg gagattgccc gccggatcaa cctgaaagtt gaaattcaga
     4441 tgcatgttgc gggcgatctc ctgtggagag atgatgttgc ttgtgccgtt ctggcgcggg
     4501 cgttgccacc cgccgccggt ttgatcgaag aggtggccgc aggtgagaaa cagatcgttc
     4561 gtgatcatcg tgccgctgcc ccagcgcgcg cccgaaacgt cgcccggatt ggtgtagatc
     4621 gaggcgaggt tggagttcca ctgcacctga caggcggcgg actggtgcgc ggcgacaaag
     4681 gcagtcgtca cgcccagcgc gccgttgtac tgttcgacgg gctgcgaatc atcgttcgcg
     4741 ccgcagatag tctccagcag agccgcatag tcgtcgccga cgccgccgtc gagacgttcg
     4801 ctggtaagtt ccaaaggttc gatgcgcgga acaaagagtg gatcgtcagc ccggtcttct
     4861 gacgaccgca cagactcctc gatgctcctg ctcccggttt cagccggacg gcgcgggttg
     4921 tccgaacccg aattttcctt actcatatga acctccgtag taaattgtgg ttgattgatc
     4981 tcttgttggt tcagctagac ccttgcccgt aggttgaggt tttggttggc agcgagacgg
     5041 attgcgcctc gtagagtttt cgatttgtaa ccgcaggcgg agcgatgtcg aagagagcgg
     5101 cctgtcgcct gtgtcggatt gccgggagtc ttgtgagtaa cccctgtagg tgcgcgggac
     5161 gcgaggattg tttgagcatc cacgccgtga ggggacagtt tcctgagcga gttgaggaag
     5221 tgcccgcgat caagaggcaa taataaaatc ggttcgctat ctcgaaagca gcccgctcac
     5281 ctttttttgt cgctgcctct aagatcgcca accgcgcctg ttatcaaggc cggaataata
     5341 cgcctgatgt ttgtgcctgt aaagagtttt ataatggtcg cattccgttc tgaatatggt
     5401 gggagaggtt gtagcggcga acgctggtag aagtgaatcg tcaggctatt tgaaacggaa
     5461 cttccgcgcc cttgagccat tcggccaaac cgccgggtaa tagatcgcga cgccccggag
     5521 cacgctcgcg ctcgacggca ccagcaggcc gaacgggtgt cgggtgagtg ctgttgttag
     5581 ctgtcgggct gagtttaccg gagggacgtt ctcgcccgtg cgtcgctcat ggcaggttgc
     5641 ccaaagcgtg cgccagcctg tatgccgcta tcagcaggtg cgcgtcctgc aatagatagg
     5701 gcttggctgt gatctcgccc aatggaataa attgcagcgc ggagtcagga tttttacccg
     5761 gcttaacttc ggctgcaaag gggtagacga cttcgggcgt cacgcctgat gtcggaaaat
     5821 atgaaccgcc caactcccac gctcgatcaa ctcgaaggtt gaagtcgcgc gccaacgctt
     5881 cctctagaaa agctggaatc tcggaatggc gtctaatcgt tgaaggtagc cgccatgccg
     5941 gagtgaccgt gatccttgag cttccgctga atgattggac agccggtagg tcgcggtgtt
     6001 cgacgccgac gaagaccccc tgttccgtcc ttatcacagg taagacgacc agagtgttat
     6061 ggctcagatg ctcaggccgc acgtattcaa atcgcgcccc ggcaagtatc cgtccctccc
     6121 tgtcacgctc cgcgaaactg ccctcatata acgagaggaa ccgtggctga gaaagcggcg
     6181 cgggttgact gaacgcggcg tgagcctgtg gcgttatgac ctgcgcggga gatgataggg
     6241 cgagcgtcaa cggctgcacg gagagactaa tgggcgcgcc gatccagggt ccataattcc
     6301 tgccgagttc ttgaagcagg cggtatatat ttatctccag cctcgcatcg aacagccccc
     6361 cgacgtggca cgcccgcaac acctgagtcg cgtccaactc acgcacgctc ccggcatctt
     6421 tgaacggggt gtagttgggg aaggtgaaat ccgtctggcc gggacgaact tcgacgaggc
     6481 gtgagatgac gagttcgtta acgccgccgg gcgaagtgta gtaagaaaaa cttccgccga
     6541 cgttgatgat atcttccgct tcaaacccgc cccgctcacg cagaatacgg cagacctctt
     6601 cttcttcgtc gccttccgaa ttgacgatgg cgctgatcgg ctcggtgatg tacccggagt
     6661 tggtcgtcga gttgaggcgg ggctggtcgg cgcaggcgtt gacgacgggg cgtggaaaat
     6721 ccttcttcgc aaacacgaag acctgtcctc ggctctcgaa ccacggcacc acgtcgagcg
     6781 tctggtgagg ccgttcgaca agctccatga cgtgtcccgt cgcttcgtgc ctgtaacctc
     6841 tgaggctcaa aaaattgggc gaagagatcg cgcgccgctc cttctcaaaa agttcgacgc
     6901 ccgcgcctgc gcgcaccttc tcgccgacga tcaggtagtt tgtcggcgga aacggtagcg
     6961 gggcttcgtc cgtgtctgaa agatagaatt taccttcaaa gcggttctgc acgatccagg
     7021 ggttccagag cgggcgcgag gtgacgatgc gcagcccacg cgaacggaac gccgcctcga
     7081 actgcgactg tgtgagatac gtgtactctt ccagaatctc cgtctcccaa tccgcgcggt
     7141 aatctttgcg cagcacgaac tccgccgccg cacgcagagc gacttcgtaa cggacaaagc
     7201 cgcgacgcgt tgactccatt ctggtgtagg gaacaggcgc gttacggttc acgctgctgc
     7261 gccagtttcg cgcgaacagt tcaaacagag cggcggcgga tagctcgcgg acatctcccg
     7321 tttcgaggcc gcccttgccc ggcaggtcga ggtagacctg ctccgccccg tcggggataa
     7381 cgaagtctct gatgatgatg acgccgccgg ttttaagttg tgcgacctga ttgtccaaag
     7441 tgtcgagcac acggttcacg tcgaagttgt tgaagctcgt gacgtgatgc agcacggagg
     7501 agtcgaggat gccatcgagc gactcggcag gaaaggccat gccggagatg tcgcccacca
     7561 cgtaagagag gttcgcgcgc cggaactgtt gcgccgcgcg ctcgaccgaa acggggttga
     7621 tgtctacgcc caccagttcc agcccgccgt agaggcaggc cagatcgtaa gtcccgcgac
     7681 cggaaccaga ccccatatcc gcgatgcggc cacgcacggg aaagtgtgcc gtggtgagcg
     7741 cgaccttctg ctgcatggag gcgtccattc cggcgaaata cgcctcgtag gctgcctgcg
     7801 tcccgcgatg ctgcttctcg tagtgtccgc tctgatttgt ggaactcatc tgtgaaagct
     7861 gccgggcgtt tgtgtgtcag ggctgcccta gcatcgtaaa tttgtataac ttgctgccgc
     7921 gcgcggtgag cgcctgccct cccgtgtgga aaagcctgtc gtcgcctctg acggaagggt
     7981 cttgaaaggt tctgatgccg gacgcgcccc tgagcctgga aaaaacttcc aacgcgccct
     8041 cgtcgttcag gtgcaggacg actccgctgt cgagcactgt aaagttaaga tcggttgaag
     8101 agacatcggg cacgagccgc gagtcgtagt cgctgaaatc tttggcgaag cggaagatga
     8161 gcttgtcgta tcgtccgcct tgtgcgccga cgaccatcag cacgttacgg gagagccgtg
     8221 cgtcgagaat ctgatagcga tcaagttcgg acaggcgaac ctgatgacac acaccgggcg
     8281 aaggaaaaat agatgcgtag taagctccca gaagattctg aatcgccaag ccctgaaaca
     8341 tccgcgtgga cttaatcata acattggcga cgggtttgat acccaacaaa atgtcgccct
     8401 tgagttcgat aaactcgacg gcaaagatgt gctcccgctg ct
//
LOCUS       DASWNS010000031         7679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_52950, whole genome shotgun sequence.
ACCESSION   DASWNS010000031 DASWNS010000000
VERSION     DASWNS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52950"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VGB76_00870"
     CDS             <1..580
                     /locus_tag="VGB76_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0097477.1"
                     /translation="QPLARRLRKKRQEETMSQSNSRLPQQAVQVPPALAAQLIGGEIN
                     IAEFVGLGRDALYAIARVGYQFLNSGRVEQARQVYRGLVAADPYDSVFRCHLAAAHHR
                     LGDLDEAFKAYTAALRFNYANVDALAGRSEIFLQRGRIAEAIADLQAVLTHDPRSQRA
                     TTLRARAILPALRNAAAQATRQPAVQSGAAGS"
     gene            complement(763..1386)
                     /locus_tag="VGB76_00875"
     CDS             complement(763..1386)
                     /locus_tag="VGB76_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097478.1"
                     /translation="MHEIGNVGGLPPQGQQTVEQPGQTGQPETVGQPGTLGQPTGIPV
                     PGQGQSLPDVLAGRDASQLVAAERMREAGGASATEKLLGLNQQPSVLGAFAAPPGNSE
                     ALRHMSPTMRRMIIRGLVAKQRTRLRRLARHLRDEREGEQRQGAQENEEGGFLDALST
                     EIVAPDAAQLARARAELTGAARMLDLLDELLVMQDYALSQMGAFAQG"
     gene            complement(1379..2464)
                     /gene="flhB"
                     /locus_tag="VGB76_00880"
     CDS             complement(1379..2464)
                     /gene="flhB"
                     /locus_tag="VGB76_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEY0097479.1"
                     /translation="MAHSGEKTERPTEKRLRDARKRGQVAKSHDLSSALLLIAALAVL
                     WLAGGYMGEWLSGAMQDQLRHAGAYQGQLTRADAFGALAGGVKAFALVLLPLFATLFL
                     VAALVGYFQVGSIFAFEPVKPNLNRLNPAESFKQKFFKGRPYLELFKTLLKMTITTLV
                     IGTVLWSSRRDVIELTRQPSSRVATYTLSLIFEIGFKVGLAFVVLGAADYFLQRFLHL
                     KELRMSKQEVKEEWKETEGNPLFKSARRQMHREILMQSMMAAVKRADVVVVNPTHVAV
                     ALQYDRATMGAPVIVAKGAELIAARIRELAKESGVPIMRDVPLARTLYELEIDSEIPE
                     ELFEAVATVLRWVYRLAEERGEVVSHA"
     gene            complement(2469..2873)
                     /locus_tag="VGB76_00885"
     CDS             complement(2469..2873)
                     /locus_tag="VGB76_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097480.1"
                     /translation="MKVKGGSPLSSGAEPLEPLDARELRKAVEGDRFAAALSQLEGQS
                     GAQGAEQAGEAKNATRAALAEIANSSNLSSSEGAASAVRESARYMIRSRLGEKYRDTE
                     QGQRVVKDLSAYVASDPLLKSKLLSILKQIRT"
     gene            complement(2942..3367)
                     /locus_tag="VGB76_00890"
     CDS             complement(2942..3367)
                     /locus_tag="VGB76_00890"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0097481.1"
                     /translation="MRLKDVQPTEEELRVVMEAGFVLREAGKFDAAEAVFRGVSELLP
                     QSDVPRVALGTVELQRGRFAEALSACDEALRLRPESLYARVHHAEALLFQQRRAEAEA
                     ELHEVIAADAQSPHSRTARALLDAADLICAQTSDAATTA"
     gene            complement(3420..3797)
                     /locus_tag="VGB76_00895"
     CDS             complement(3420..3797)
                     /locus_tag="VGB76_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097482.1"
                     /translation="MAVSNDPMINQMPTVQPAPSALPPIQPQPKQPGKFSRIFGGLVG
                     GALNIVAPGAGSLIGGFINGGAGGVDFNNMQSMLNQQAQQSMQMLAVQNRVQSQTQEF
                     TTVSNLLKAKHDGDMSAVHNFKS"
     gene            complement(3956..4270)
                     /locus_tag="VGB76_00900"
     CDS             complement(3956..4270)
                     /locus_tag="VGB76_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097483.1"
                     /translation="MNRYLNGSEDATASTQNGAATGSSDLSSLYHYPSLGALFEQPNS
                     PQLAEMRGRFSRSTQALERVVRQGTKEDAERASRALRAYEAVSGLFDELQKLQQGQAT
                     QG"
     gene            4466..5542
                     /locus_tag="VGB76_00905"
     CDS             4466..5542
                     /locus_tag="VGB76_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0097484.1"
                     /translation="MTPKLLIKRRGDAPSAAPQSELALDGALISVGTDPAATLSLRGA
                     NIAPEQAVIINEDGQLLLINRADGTTLNDEPLPREARRPLTHGDRLGVGNYVIILVTD
                     EAHTASEKVARQGNFSESLTLSTDATAQVETTLAPTQPVEPPPNGAERAPRNFAAILD
                     TLRTEEDSFYLLIEGGADDGQRVRLESLEMPLGWEETGQRIAFEAEKVLAQRAVLRKD
                     WTGVFVQASGVGLVAVNGEPVEAPRQLRNGDRLTLVPTVNTAAQNQAILIFHEPASLI
                     ALDSLLSQKLPPPVALQPAAPDTSLIAQADADAALEVSRERTARPVATRKYFGYFTRG
                     ELLLMLVGTLITAAIIFLVLEFTA"
     gene            5593..>7679
                     /gene="sctV"
                     /locus_tag="VGB76_00910"
     CDS             5593..>7679
                     /gene="sctV"
                     /locus_tag="VGB76_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctV"
                     /protein_id="HEY0097485.1"
                     /translation="MDGLLKQLARFWQALKSGDVLRLLARHNDLVLPIALMLAISMLF
                     IPIPTPLISALLVLNLAISFIVLATSLYISSPVQLTSYPTILLLTTLFRLCLSVSVSR
                     SILERAYAGEVIETLGKFTGGGNVIVGAVVFVMILVVQFIVVAKGSERVAEVAARFTL
                     DAMPGKQMSIDADMRSGLLTQEQARKMRGALQKESQLYGAMDGAMKFVKGDSIATIVI
                     ALINIAAGLAIGMLYKGLALPQALQKYTIMTIGDGLAAIISSILITVSAGIVVTRVAA
                     DEDKSNVGSDISLQLFHNPKPLVIAAALLLVLALIPGMHLPFFVVGLIVGGVAYLLYR
                     AQQRAARDEAERIAKTGATGGRDELQPTFAVPLAVVVSSPLTHLIDQQTPSGALFRQE
                     LPKLRSALYYDLGVMLPNVYVSGDAPLKPDQYFIAVKEVPIAYGRLKPDSVYVNDSAE
                     NIKVFGLEGEDVRNPADLSPGSWIPAAQRPIAELAGLKVWTPVEVISLHLSRVMNRYA
                     HEFIGIQEAQALLDFVARGVPKLVEEVVPKVVSIHQFTDVLQRLVQEGISVRDVKSIL
                     DALSEWGRIEKDPMMLTEYVRASLKRYISFRYTQGRDALFVYLLDPEIEDVIRGAIRR
                     TPTGSFLSLDPSIAHDILEALRREIANLPPTAQKPVVITDMELRRFVRKMVELEFPTL
                     AVLSYQELTPELNV"
BASE COUNT         1345 a         2588 c         2304 g         1442 t
ORIGIN      
        1 gcaaccgctc gctcgccgtc tgaggaagaa gaggcaggaa gaaaccatgt cgcaatcgaa
       61 cagccgtctg ccgcaacaag ccgttcaagt gccgcccgcg ctcgccgcgc aactgatcgg
      121 cggcgagatc aacatcgccg aatttgtcgg cctcgggcgc gacgcgctct acgccatcgc
      181 gcgcgtcggc tatcagtttt tgaacagcgg gcgcgtcgaa caggcgcgac aggtttatcg
      241 cggcctcgtc gcggcagacc cttacgacag cgtctttcgc tgtcacctcg ccgccgcgca
      301 tcaccggctc ggcgacctgg acgaagcgtt caaagcgtac acggcggcgt tgcgcttcaa
      361 ctacgccaac gtggacgcgc tcgccggacg aagcgagatt tttctccaac gcgggcgcat
      421 cgccgaggcc atcgcagacc ttcaagccgt gctcacacac gacccgcgca gccagcgtgc
      481 gaccaccctg cgcgcgcgcg ccatcctccc ggcattgcgc aacgccgccg cgcaagcgac
      541 gagacagccc gctgtgcagt cgggcgcggc aggttcgtag cgcagagcgc cgcgagaacg
      601 tgcggcagac gacatagcga agcccctgaa agtggcgaaa gagagtcaac gtcaaacgtg
      661 attcgccgct ttcaggggct tctgaatttt gggtagtgtc ctgagttgat ttgaactctc
      721 cgtgcgccct ccgtgcgctc tgtgactctg aggaaagctg actcatccct gcgcaaacgc
      781 gcccatctgg ctgagcgcgt aatcctgcat caccagcaac tcgtccaaca ggtcgagcat
      841 tcgcgctgcg cccgtcaact cggcgcgtgc gcgcgccagt tgcgcggcat cgggcgcgac
      901 aatttccgtc gagagtgcgt cgaggaatcc gccctcttca ttctcctgcg ccccctgccg
      961 ttgttcgcct tcacgctcgt cgcgcaggtg gcgcgcgagg cggcgcaggc gcgtgcgctg
     1021 ctttgccacg aggccgcgaa tgatcatgcg ccgcatcgtg ggcgacatgt ggcgcagggc
     1081 ttcgctgttg ccgggcggcg cggcgaacgc acccaacacg gagggctgtt gattcagccc
     1141 caataatttc tccgtcgcag acgcgccgcc cgcctcacgc atccgctccg ccgcgaccaa
     1201 ctgcgaggcg tcgcgcccgg ccagcacgtc cggcaggctt tgtccctgcc ccggcacggg
     1261 gatgcccgtc ggctgaccaa gcgtgcccgg ctgcccgacc gtctccggct gtcccgtctg
     1321 ccccggctgt tcgaccgtct gctgcccttg cggcggcaag ccgcccacgt tcccaatctc
     1381 atgcatggct caccacctcc ccgcgttcct ctgccagtct gtacacccag cgcaacactg
     1441 tcgccaccgc ttcaaacagt tcttcgggaa tctcgctatc aatctccaac tcgtacaggg
     1501 tgcgtgcaag cggcacgtcg cgcatgatgg gcacgcctga ctctttcgcc aactccctga
     1561 tgcgcgccgc gatcaactcc gcgcctttcg cgacgatgac gggcgctccc atcgtcgcgc
     1621 gatcatactg gagcgcgacg gccacgtgcg tcgggttgac cactaccacg tccgcgcgct
     1681 tgactgccgc catcatgctc tgcatcaaga tttcgcggtg catctgccgc cgtgcgctct
     1741 taaacaaagg gttgccctcc gtctctttcc actcctcttt cacctcctgc ttgctcatcc
     1801 gcagttcttt gaggtgaagg aatctttgca ggaagtagtc cgccgcgccg agcacgacga
     1861 aggccagccc gaccttgaag ccgatctcga agatgagcga gagcgtatag gttgcaacgc
     1921 gcgacgaagg ctgtcgcgtc aactcgatca cgtcgcgccg cgacgaccag agcaccgtgc
     1981 cgatcacgag cgtcgtgatc gtcatcttca acaaagtctt gaagagttcg aggtagggac
     2041 gacccttgaa gaacttctgt ttgaagctct ccgccgggtt caggcggttg aggttcggtt
     2101 tgacgggttc aaaggcgaag atcgagccga cttgaaaata gccgaccagc gcggcgacga
     2161 ggaacagcgt ggcgaagagc ggcagcagca cgagcgcgaa cgccttgacg ccgcctgcga
     2221 gtgcgccgaa ggcgtcggcg cgcgtcagtt ggccttgata ggctccggcg tgccggagtt
     2281 gatcctgcat cgcgccgctc aaccattcgc ccatgtagcc gcccgcgagc cagagcacgg
     2341 cgagcgcggc tatgagcagc agggcggacg agaggtcgtg acttttcgcc acctgcccgc
     2401 gcttgcgtgc gtcgcggagg cgtttctcag tcgggcgttc agttttctcg ccgctgtgcg
     2461 ccatctcatc acgtgcggat ttgcttgagt atggagagga gttttgactt caacagcgga
     2521 tcggaggcga cgtacgcgct caggtctttg acgacccgct gcccctgttc cgtgtcgcga
     2581 tacttttcgc cgaggcgcga cctgatcatg tagcgcgccg attcgcgcac cgccgaggcc
     2641 gcgccttcgc tgctcgataa attcgacgag ttggcgattt ccgcgagggc ggcgcgcgtc
     2701 gcattcttgg cctcgcccgc ctgttccgcc ccctgcgctc cgctctgtcc ttcgagttgg
     2761 gagagcgcgg cggcgaagcg gtcgccctcg accgccttgc gtagctcgcg cgcgtcgagt
     2821 ggttccagcg gctccgcgcc gctcgacagg ggtgatccgc ctttgacttt catctgactt
     2881 tcatcctgag cgtcctgcaa agagaaagat cgccgacgct agcgtcgcaa ttcatgaaga
     2941 ttcaggcagt ggttgcggcg tcgctcgtct gcgcgcagat gaggtcggcg gcgtcgagca
     3001 gggcgcgcgc cgtgcggctg tgcggcgatt gcgcgtcggc ggcgataact tcgtgcaact
     3061 cggcttcggc ctccgcacgc cgctgctgga acaggagcgc ctccgcgtga tggacgcgcg
     3121 catagaggct ttcggggcgc aggcgcagag cctcgtcgca agccgataac gcctccgcga
     3181 aacgcccgcg ctgcaactcg accgtaccga gcgcgacgcg cggcacgtcc gactgtggca
     3241 gcagttccga cacgccgcga aagactgcct cggcggcgtc gaacttcccc gcctcgcgca
     3301 gcacgaaccc ggcctccatg acgacccgca gttcttcctc ggtgggctgc acgtccttga
     3361 gcctcatcct ttttcacctc tcgcccgcgt agctgtcaac gccgtgcgcc ttccagtgtc
     3421 tacgacttga agttatgcac cgccgacatg tcgccgtcgt gcttggcctt caagagattc
     3481 gagacggtcg tgaactcctg cgtctgcgac tggacgcggt tctggacggc gagcatctgc
     3541 atggactgct gcgcctgctg gttgagcatg gactgcatgt tattgaagtc cacgccgccc
     3601 gcaccgccgt tgatgaagcc gccgatgagg ctgcccgccc ccggcgcgac gatgttgagc
     3661 gcgccgccga cgaggccgcc gaagatgcgc gaaaacttgc cgggctgttt cggctgcggc
     3721 tggatgggcg gcaacgcaga cggtgcgggc tgcacggtcg gcatctggtt aatcatcgga
     3781 tcattgctga ctgccatgtt ctacctcccc cggtctgttg ccggacgcga agaaaaggct
     3841 ctcccgcaca ttttacgagg ccgtgctctc cgctcgaaat aaaagagatt ctctgctgtc
     3901 gaaaggatta tcggcggttt gcggaaaaag tttccggttg cgccgaaaaa aattttcagc
     3961 cctgtgtggc ctgcccctgc tgcaattttt gcagctcgtc gaacagcccc gaaaccgcct
     4021 cgtaggcgcg cagggcgcgt gaagcgcgtt ccgcatcctc cttcgtgccc tgccgcacga
     4081 cgcgttcgag cgcctgcgtg ctccggctga aacgcccgcg catctctgcc agttgcgggc
     4141 tgttaggctg ctcgaacagt gcgccgagcg aagggtagtg atagagcgaa gatagatcgg
     4201 acgagcctgt cgccgcgccg ttttgggtgg acgccgtcgc gtcttccgac ccgtttaagt
     4261 acctgttcat cgggctaacc tcctgcggga agcgtgcgct cggattgagt tgtaaagcgg
     4321 atgcgacggt gtgatagtat taagtgcaag taattgatgt caaacagacg aagcacttcc
     4381 cccgtttgtc tccgaagtaa gttatggata gtgcaatttt ccgaatgtct ccgggggcac
     4441 tcgccggacg acccgcaccc gttgcatgac gccgaaactg ctcatcaaac gccgggggga
     4501 cgcgccttca gccgcgccgc agagcgagtt ggctctcgac ggcgcgttga tttccgtcgg
     4561 caccgacccg gctgccacgc tctccctgcg cggcgcgaac atcgcccccg aacaggctgt
     4621 catcatcaac gaagacggcc aactcctcct catcaatcgt gccgacggca caactctgaa
     4681 cgacgaaccc ttgccccgcg aagcgcgccg cccgctcacg cacggcgacc gtctcggcgt
     4741 cggcaattac gtcatcatcc tcgtcacgga cgaagcacat acagcgtccg agaaggtcgc
     4801 acgtcagggc aacttttccg agtctctaac gttaagcacg gacgcgacgg ctcaagtgga
     4861 gacaactctt gcgccgacac agcccgtcga gccgccgccc aacggagccg agcgcgcacc
     4921 gcgcaacttc gcggccattc tcgacacgct ccgcacggag gaagatagct tctaccttct
     4981 catcgaaggc ggcgcggacg acgggcagcg cgtgcggctc gaatcgctgg agatgccgct
     5041 cggctgggaa gagacaggtc agcgcatcgc cttcgaggcc gaaaaagttt tggcgcagcg
     5101 cgccgtgctg cgcaaggatt ggacgggcgt gtttgtgcag gcatccggcg tcggcctcgt
     5161 cgccgtcaac ggcgagccgg tcgaagcccc gcgccaactg cgcaacggcg accgcctgac
     5221 gctcgtgccg accgtcaaca cggcggcgca gaatcaagcc atcctgatct ttcacgaacc
     5281 cgcgtcgctc atcgcgctcg actcactgct gtcgcaaaag ttgccgccgc cggttgcgct
     5341 ccaacccgcc gcgcccgata cgagtctcat cgcgcaggcg gacgcagatg cagcgttgga
     5401 agtgtcgcgc gaacgcacgg cgcgtcctgt tgcgacgcga aaatacttcg gctacttcac
     5461 gcgcggcgag ctgttgctca tgctcgtcgg cacgctcatc acggcggcga ttatttttct
     5521 cgtcttggag ttcacggcgt aggacgcgcc gcgcggcgac gcgccggttc acaggcgcaa
     5581 gagtgaagtg aaatggacgg gcttctcaaa caactcgcac ggttctggca ggctttgaag
     5641 agcggcgacg tgctgcgcct gctcgcgcgt cacaacgatc tcgtgctgcc aatcgcgctg
     5701 atgctcgcca tcagcatgct ctttatcccg atcccgacgc cgctcatctc cgcgctgctc
     5761 gtgctcaacc tggcgatctc gttcatcgtg ctcgccacct cgctctacat ctcaagcccc
     5821 gtccaattaa cttcgtaccc gaccatcctg ctcctgacga cgctctttcg tctctgcctg
     5881 agcgtctccg tctcgcgcag catcctcgaa cgcgcctacg cgggcgaagt catcgaaacg
     5941 ctcggcaagt ttaccggcgg cggcaacgtc atcgtcggcg cggtcgtctt cgtgatgatc
     6001 ctcgtcgtgc agttcatcgt ggtcgccaaa ggctcggagc gcgtggcgga ggtggcggcg
     6061 cgcttcacgc tcgacgcgat gccgggcaag cagatgtcca ttgacgcgga tatgcgctcc
     6121 ggcctgctga cgcaggagca ggcgcgcaag atgcgcggcg cgctgcaaaa ggagtcgcaa
     6181 ctctacggcg cgatggacgg cgcgatgaag ttcgtcaagg gcgactccat tgccaccatc
     6241 gttatcgcgc tcatcaacat cgcggcggga ctcgccatcg ggatgctcta taaggggctg
     6301 gcgctcccgc aggcgttgca gaagtacacc atcatgacca tcggcgacgg gctggcggcg
     6361 atcatctctt cgatcctgat caccgtctcg gcgggcatcg tcgtgacgcg cgtagctgcc
     6421 gacgaagaca agtcgaacgt cggctcggac atcagcctgc aactcttcca caatccgaag
     6481 ccgctcgtca tcgccgccgc gctgctgctc gtgctcgccc tgattccggg catgcacctc
     6541 cccttcttcg tcgtcggcct gatagtcggc ggcgtcgctt acctgctcta ccgcgcgcag
     6601 caacgcgccg cccgcgacga agccgaacgc atcgccaaga ccggcgcgac cggcgggcgc
     6661 gacgagttgc aaccgacctt cgccgtgccg ctcgccgtcg tcgtcagttc gccgctcacg
     6721 cacctcatag accagcagac gccttcgggc gcgctcttcc ggcaggagtt gcccaagctg
     6781 cgttcggctc tctactacga tctgggcgtc atgctgccca acgtctacgt gagcggagat
     6841 gcgccgctca agcccgacca gtatttcatc gcggtcaagg aagtgcctat cgcctacggg
     6901 cggctcaaac cggatagcgt ctacgtcaac gactccgccg agaacatcaa ggtcttcggc
     6961 ctcgaaggcg aggacgtgcg caaccccgca gacctctcgc ccggctcatg gattcccgcc
     7021 gcgcaacgtc ctatcgccga actcgccggg ctgaaagtct ggacgcccgt ggaagttatc
     7081 agcctccacc tttcgcgcgt gatgaacagg tacgcgcacg agttcatcgg catccaggag
     7141 gcgcaggcgt tgttggattt cgtcgcgcgc ggcgtgccca aactggtcga agaggttgtg
     7201 ccgaaggtgg tcagcattca ccagttcacg gatgtcctgc aaaggctcgt gcaggagggc
     7261 atctccgtgc gcgacgtgaa atccatcctc gacgcgcttt ccgaatgggg gcgcatcgaa
     7321 aaagacccga tgatgctgac cgagtacgtg cgcgcctcgc tcaagcgtta catctccttc
     7381 cgctacacgc agggacgcga cgcgctcttc gtctatctac tcgaccccga aatcgaagat
     7441 gtgattcgcg gcgcgatccg ccgcacgccg accggctctt ttctctccct cgacccgtcc
     7501 atcgcacacg atattctcga agccctgcgg cgcgagatcg ccaacctccc gccaaccgcg
     7561 caaaagcccg tcgtcatcac cgacatggaa ctcagacgct ttgtgcgcaa gatggtcgag
     7621 ttggaattcc cgacgctcgc cgtgctctct tatcaggaac tcacgccgga gttgaacgt
//
LOCUS       DASWNS010000032        10586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_286696, whole genome shotgun sequence.
ACCESSION   DASWNS010000032 DASWNS010000000
VERSION     DASWNS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10586
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286696"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VGB76_00915"
     CDS             <1..640
                     /locus_tag="VGB76_00915"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY0097486.1"
                     /translation="NKIAEALPEVLTTTLDSIVNWARKSSLWPATFGLACCAIEMMNA
                     TSSRHDLARFGSEVFRASPRQADVMIVSGRVSRKMAPVLRRIYDQMPEPKWVISMGAC
                     ATSGGVFDNYAIVQGVDKIVPVDVYVPGCPPRPEMLINAVMMLQDKVMKESLRDRRDI
                     PAEQAREAVPPGTLPASGVSALNEAAAQDADEKANVSRPYTIPSRHERRRSS"
     gene            753..1298
                     /locus_tag="VGB76_00920"
     CDS             753..1298
                     /locus_tag="VGB76_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY0097487.1"
                     /translation="MSKDIEREQNIAGQAASMPARDQVGAELIGGLQESHPGAVLEVI
                     QAFGEITLVVPRERIVELCDYLKRTPEFAFNLLADICGADRGPEEDPRFEVNYHLFST
                     TKFHRLRLKVVVSEEDLHVPTVTGIWRTANWHERETFDMFGIRFDGHPDLRRILLPDD
                     WQGHALRKDFPLRGYEPYSLG"
     gene            1384..2604
                     /gene="nuoD"
                     /locus_tag="VGB76_00925"
     CDS             1384..2604
                     /gene="nuoD"
                     /locus_tag="VGB76_00925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEY0097488.1"
                     /translation="MSASTTNLPPQFEVVDQPFDTQMTISMGPQHPSTHGVLRLELVL
                     DGEMVVKCTPDIGYLHTGMEKLFEYKKYQQGIVITDRMDYLNPLGNNLCYVMAVEKLL
                     GLEIPERAQVIRVLMCELQRIASHMVWLGTHALDIGAMTVFFYAFREREKILNLIEAA
                     SGGRLTPSYFRIGGLMMDLPAGFERRAKQFMDGFPGAIDEFETLLSGNRIFQTRTQGV
                     GIINAEDAIDMGVTGPCLRGSGVAIDVRRTNPYTGYETYDFEIPVEQSCDVWARYIVR
                     MRELRESLKIARQALGRLKPGAVKADAPKVVLPDREAMKTHMDALIHHFLIVAEGFNV
                     PAGEVYHPIEGSKGELGCYVRSDGGPKPARVHFRGPSFINLSALPHMAEGEMVADVVA
                     IIGSLDIVLGEIDR"
     gene            2694..3251
                     /gene="nuoE"
                     /locus_tag="VGB76_00930"
     CDS             2694..3251
                     /gene="nuoE"
                     /locus_tag="VGB76_00930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY0097489.1"
                     /translation="MSKQFTPNNSLPQEAPVPDEIAQFSAAVEAEIDGHLAKYPLKRS
                     AILPIMFIVQRERGYLDKPGVAYLADRLGVRVTDIWEVATFYSMLNTEPVGKYHLQVC
                     RTLSCKILGADKITEHCASRLGIKPGETTEDGQFSLSEVECLGSCGTAPMLQIGFDYY
                     ENLTPEKVDELIEQCRKGQITNGVR"
     gene            3330..4646
                     /gene="nuoF"
                     /locus_tag="VGB76_00935"
     CDS             3330..4646
                     /gene="nuoF"
                     /locus_tag="VGB76_00935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY0097490.1"
                     /translation="MFIGAIGCEPLQLARVHLERSQSLETYQQHGGYEALRRVLGGMS
                     PDDVIAEVKKSALRGRGGAGFPTGMKWSFVPKDSPKPKYVVCNADESEPGTFKDRYIM
                     ERDPHMLIEGMLIAAYALGSKACYIYTRGEYRYLIEIMDRAIEEARAAGLVGEKIAGT
                     DFACEVYTHTGAGAYICGEETALLNSLEGLRGHPRMKPPFPAVAGLYGCPTVVNNVET
                     LTAVPWIILNGGEAYQKLGTEKSGGTKLWSVSGHVNRPGVYELPMGYADMERFIMEDC
                     QGIPNGRNLKAVIPGGSSVYIMPASDIIGKSVKMDYEGLVAGGSMVGSGGFIVMDDTV
                     DIFESTKNLTEFYKHESCGWCTPCREGTDWLVKVFKRIARGEGKPDDAQLLLDLCDNI
                     EGKSFCPLGDAASWPIQSAIKKFPEDFRRHLAPLAGGNGHRSLSTM"
     gene            4674..5105
                     /locus_tag="VGB76_00940"
     CDS             4674..5105
                     /locus_tag="VGB76_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097491.1"
                     /translation="MRPRQLWRAVAFSAALFAAPAPRVEADGVRATRSPAPAQCRRTD
                     RAIRKSIRIGHGSTVVRKDTVRLCTSHEYRFDADAGEKISINLATGKRTSFTLRTPSG
                     DIVEGADGVKNWSGDAPEVGDYLITIGTDATAAYTLSVTLG"
     gene            5149..7446
                     /locus_tag="VGB76_00945"
     CDS             5149..7446
                     /locus_tag="VGB76_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0097492.1"
                     /translation="MSTELIKVTINGNLYEVEKGARLIDVCREKGHAVPSFCYYADLA
                     LQASCRMCLVRIEKMPKLQTSCTITCTDGMSVTTESDEIEKAQRGMVEFLLANHPLDC
                     PVCDRGGECELQEMVFDWGGLEERFQEKKNYYPEKFLSPIVANDPQRCILCKRCTRVC
                     DEWMGEDAIEAGGRGANTVIGTFDGWLNCSQCGNCIEVCPTGTLLDATYRHQARPWEL
                     SQTITTCNFCSDGCQMSLGSRAGEVMRSVARDRYVNGINGEFLCIKGRFAHPFINHEE
                     RIKTPLIRYKKGGKLIPATWDEAIRHTAAKLQAIKDAHGADALGVVGSPRLTNEANYA
                     LVKFAREVVGTQNYTATDAFSLAPFFASLSAPLATHRDIRHAKTIIIIGGEPEELQPL
                     TGRQIRQAVRNGGAKLVIVNSVPIRLKQAATQFIHINPGAEDAAVLAFAGSEYATAAH
                     KSGIEAQEFEDARRIVEETTGDVVVIFGELSATAQSALANIPTNYGEREGRRFLFHPL
                     PLFNNSLGVHDLGMMGGALDANAMLDAAGTTLRALYVAGSFLPQHFAGRADALSKLDF
                     LVVQELFETETTAHADVVFPAASYAETDGTFTNNSGLVQRVRQSIPPVHQSRPDWMIT
                     DALAAALGVNFDFQWSAGKVFLELARNTQAYAGMKYALLKDESQPVQARHAVAGDNGG
                     AADVLAELRERVEALSVEGEKIMETPPIGHELFKPGTLTGKTPQFPLLDAGNPKPPTV
                     LVSPLYQISVDPALRRATVVNAAGD"
     gene            7554..8636
                     /gene="nuoH"
                     /locus_tag="VGB76_00950"
     CDS             7554..8636
                     /gene="nuoH"
                     /locus_tag="VGB76_00950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY0097493.1"
                     /translation="MDWNTLTYVGLKMLGINIAFVVVLMIVAYTVLAERRILALIQGR
                     LGPNRVGYGGILQPFADLLKFIFKEEIVPDKATKFVYFLAPMIAIVGALMTIIVYPFG
                     PEVNLPFIGTIKLVVTQFDVGLLYVLAITSIGVYGIALAGWSSNNKYSLMGGLRASAQ
                     LISYELSLGLGLVGVVLLSGTLDLYQIVEQQAGYWWVGGVVPIPRWNIFPQLLGFIVY
                     LISAIAETNRVPFDLPEAETELVAGFHTEYSALKFALFFMAEYVNMFTVSMLAATLFF
                     GGWNFPWLDLLVARGGFAFAFLSLLAFLAKVLFFLFLYIWLRGTLPRFRFDQLMNFGW
                     KFLLPAAILNVVLTATIVFAFMTYRS"
     gene            8706..9380
                     /locus_tag="VGB76_00955"
     CDS             8706..9380
                     /locus_tag="VGB76_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY0097494.1"
                     /translation="MTTLLFVLLAGLAIGSAIAMVVQRNPLYSAISLIGVFIALAGLY
                     LTLAAPFLAAIQIIVYAGAIMVLVVFVIMLLNVEEEEHKPLRLGYLTAVAILLAAILF
                     GEAGFILYFMGRSGVEPLDPNAVGNASNVGLTASIGTGLFTNYLLPFEITSVLLLMAI
                     VGAMTLARRVGLRPTDLAIPGSQVIPRHSIEPKAVGDATEVVSTTTADAERALGLEGA
                     RHRREE"
     gene            9397..9705
                     /gene="nuoK"
                     /locus_tag="VGB76_00960"
     CDS             9397..9705
                     /gene="nuoK"
                     /locus_tag="VGB76_00960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY0097495.1"
                     /translation="MPEVSLSWYLALSAVLFTIGVAGVIIKRNAIAMFMCIELMLNAV
                     NLTFVAFSSYFRDVSGQLFVFIVMTVAAAEAAVGLGIILAVFRNRESLDVDDSSVLKN
                     "
     gene            9796..>10586
                     /locus_tag="VGB76_00965"
     CDS             9796..>10586
                     /locus_tag="VGB76_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY0097496.1"
                     /translation="MLRYIILAPLVGAAINWLVGRRVRSEAFIGAVASLSIAVSCALS
                     LWLAFGPNGALVGEPKVILDHVWTWMEAGTFRADIGFAMDRLSGIYACFITFVGLLIH
                     VFATGYMHGDKSFYRFFAYLNLFMFMMLTLVLADNLLLMFVGWEGVGLCSYLLIGYYI
                     DRREAGDAAKKAFVANRVGDWGVVLGIMLVFAITQAAGHASISFFDKPGVVSALKTIN
                     AMGVEAFSPGTLIAGGITSAALLLFIGATGKSAQIPLFVWLPDAMA"
BASE COUNT         2033 a         3191 c         3253 g         2109 t
ORIGIN      
        1 aaataagatc gcggaagcgt tgccggaagt tttgacgacg acgctggatt caatcgtcaa
       61 ctgggcgcgg aagtcttccc tgtggccggc aactttcggg ctcgcctgtt gcgccatcga
      121 gatgatgaac gcgacgagtt cgcggcacga cctcgcgcgt ttcggctccg aagtgtttcg
      181 cgccagcccg cgtcaggccg acgtgatgat cgtctccggt cgcgtctcgc gcaagatggc
      241 tccggtgctg cgacgcatct atgatcagat gcccgaaccg aaatgggtta tctcgatggg
      301 cgcgtgcgcc acttcgggcg gcgtcttcga caactacgcc atcgtgcagg gcgtggataa
      361 gatcgtgccc gtcgacgtat acgtgccggg ctgcccgccg cgcccggaga tgctgatcaa
      421 tgccgtgatg atgttgcagg acaaggtgat gaaagagtct ctgcgcgacc gcagggacat
      481 cccggcagaa caggcgcgcg aagccgtccc gccgggcaca ctcccggcct ccggcgtctc
      541 cgcgctcaac gaagcggcgg cgcaagacgc ggacgaaaaa gcgaacgtct cgcgcccgta
      601 cactattccg tcgcggcacg aacggcgcag gtcgtcatag agcagtgacg ggcgacaagg
      661 gacaggtgac aagttaaaac agaaaacctg ttcttaaact tctcctgttt cttgctcgtc
      721 acactttact cgtcacttgt cactggtttc ttatgtctaa agatatagag cgcgaacaga
      781 atattgccgg gcaggcggcg agcatgcccg cgcgggatca agtgggcgcg gagttgatcg
      841 gtgggttgca ggagtcgcat ccgggcgcgg tgctggaggt gattcaggcg tttggcgaga
      901 tcacgctcgt cgtgccgcgc gagcgcatcg tagaactttg cgactatctc aaacggacgc
      961 cggagttcgc tttcaacttg ctggcggaca tttgcggcgc ggatcggggg ccggaggaag
     1021 acccgcgatt tgaagtcaac tatcatcttt tttccaccac taagtttcat cgcctgcggc
     1081 tgaaagtggt cgtgagcgaa gaggatttgc acgtgccgac cgtgactggc atctggcgca
     1141 cggccaactg gcacgagcgc gaaactttcg acatgttcgg catccgtttc gacggacatc
     1201 ccgatttgcg gcgcatcctc ttgcccgacg actggcaagg ccacgcgctt cgtaaagact
     1261 tcccgctgcg cggctacgag ccgtacagcc tcggctaaga agtgatgagt gatgagtgat
     1321 gagtgatgag tcaaaactga cttcttactc atcactcatc actcatcact catcacttaa
     1381 agtatgagcg cttctaccac aaacctaccg ccgcagtttg aggttgtcga tcagccgttc
     1441 gacacgcaga tgacgatctc gatggggccg cagcaccctt cgacgcatgg cgtcctgcgt
     1501 ctcgaactcg tgctcgacgg cgagatggtc gtcaagtgca cgcccgacat cggctatctc
     1561 cacacgggga tggagaagct cttcgaatat aagaagtatc agcaaggcat cgtcatcacc
     1621 gaccggatgg attatctgaa tccgctcggc aacaatctct gctacgtgat ggcggtggag
     1681 aaactgctcg gccttgagat accggaacgc gcgcaggtga ttcgcgtcct gatgtgcgag
     1741 ttgcagcgca tcgcttcgca catggtctgg ctgggaacgc acgcgctcga catcggcgcg
     1801 atgacggtct tcttttacgc cttccgcgaa cgcgagaaga ttctcaacct gatcgaggcc
     1861 gcatcgggcg ggcgtctgac gccctcctat ttccgcatcg gcggcttgat gatggacttg
     1921 cccgccggat tcgagcggcg cgcgaagcag ttcatggacg gctttcccgg cgcaatcgac
     1981 gaattcgaga cgctgctctc cggcaaccgt atcttccaga cacgcacgca gggcgtcggc
     2041 atcatcaatg ccgaagacgc gattgacatg ggcgtcacag gcccctgcct gcgcggcagc
     2101 ggcgtcgcca tagacgtgcg gcgcacgaac ccttacacgg gttatgagac ttacgacttc
     2161 gagattcccg tcgagcagag ttgcgacgtg tgggcgcgct acatagtacg catgcgcgaa
     2221 ctgcgcgagt ctctgaagat cgcgcggcag gcgttggggc gtctgaaacc gggcgcggtt
     2281 aaagcggacg cgccgaaagt cgtcctgccc gacagagagg cgatgaagac gcacatggat
     2341 gcgttgatcc atcacttcct gatcgtggcc gaaggcttca acgtccctgc gggcgaggtg
     2401 taccacccga ttgaaggttc gaagggcgaa ctcggctgct acgtgagatc ggacgggggg
     2461 ccgaagcccg cgcgcgtgca ttttcgcggc ccctcgttca tcaacctctc ggccttgccc
     2521 cacatggcgg aaggcgagat ggtcgcagac gtggtcgcca tcatcggctc actcgacatc
     2581 gtgctcggcg aaattgatag ataagaattc agggggcagc attcaggaat cagaataaac
     2641 agcacgacca gcttgattcc gacttctgaa ttctgcctcc tgactcctga cttatgagca
     2701 aacagttcac gccaaacaat tctttgccgc aggaagcgcc cgtgcctgat gagatcgcgc
     2761 aattttcggc ggcagtggag gcggagatcg acgggcatct ggcgaagtat ccgttgaagc
     2821 gttcggcgat cctgccgatc atgttcatcg tgcagcgcga gcgcggctat ctggacaagc
     2881 cgggcgtggc ctatctggcg gatcgtctgg gcgtgcgcgt cacggacatc tgggaagtgg
     2941 cgacgttcta ctcgatgctc aacacggagc cggtcggcaa gtatcacttg caggtctgcc
     3001 gcacgctctc gtgcaagatt ctcggggcag acaagatcac ggagcactgc gcgagccgtc
     3061 tcggcatcaa gccgggcgag acgaccgagg acgggcagtt ttcgctctcg gaggtggaat
     3121 gtctcggctc gtgcggcacc gccccgatgt tgcagatcgg ctttgactat tacgaaaatt
     3181 tgacgccgga gaaagttgac gaactgatcg agcaatgtcg caaagggcag atcacgaacg
     3241 gcgtccgctg aagcgtctga ggccgaaggt tctgtagttt tgatttccga agtttaactg
     3301 attcgagttt tagtctcctg ttaaagaata tgttcattgg tgccatcggc tgtgaaccgc
     3361 tccaactcgc gcgcgtccat ctggagcgtt cgcagtcgct cgaaacttat caacagcacg
     3421 gcggctatga agccctgcgc agagtgctcg gcggaatgtc gcccgacgac gtgatcgcgg
     3481 aagtcaagaa gtccgcgctg cgcgggcgtg gcggcgcggg tttcccgacg gggatgaagt
     3541 ggagtttcgt cccgaaggat tcgccgaagc cgaagtacgt cgtctgcaac gcagacgaat
     3601 cggagccggg cacgttcaaa gatcgctaca tcatggagcg cgacccgcac atgctcatcg
     3661 agggtatgtt gatcgcggcc tacgcgctcg gctcgaaggc ttgctacatc tacacgcgcg
     3721 gcgagtaccg ttatctcatc gagatcatgg atcgcgcgat tgaagaggca cgcgcggcgg
     3781 gactcgtcgg cgagaagatc gcgggcacag acttcgcgtg cgaggtctac acgcacacgg
     3841 gcgcgggggc atacatctgc ggcgaagaga ctgcgctcct gaattcgctc gaaggcttgc
     3901 gcgggcaccc gcgcatgaag ccgcccttcc ctgccgtcgc cgggctttac ggctgcccga
     3961 cggtcgtgaa caacgtcgag actctaaccg ccgtgccgtg gatcattctc aacggtggcg
     4021 aggcttacca gaagttgggg acggagaaga gcggcggcac gaaactctgg tctgtgagcg
     4081 gccacgtgaa tcgccccggc gtctatgaac tgccgatggg ctacgccgac atggaacgct
     4141 tcatcatgga agattgccag ggcatcccga acgggcgcaa tttgaaggcg gtgattcccg
     4201 gcgggtcgag cgtctacatc atgcccgcct cggacatcat cggtaagagc gtgaagatgg
     4261 actacgaggg tttggtcgcg ggcggttcga tggtcggctc gggcggcttc atcgtgatgg
     4321 acgacacggt tgacatcttc gaatccacga agaacctgac ggagttttac aaacacgaat
     4381 cctgcggctg gtgcacgccc tgccgcgaag gcacggactg gctcgtcaag gtcttcaaac
     4441 gcatcgcacg cggcgaaggc aagccggacg acgcgcaact gctgctcgac ctgtgcgaca
     4501 acatcgaagg caagtcgttc tgcccgctcg gcgacgccgc ttcctggccg atccagtcgg
     4561 ccatcaaaaa gtttcccgaa gacttccgac gccatctcgc gccgctcgcg ggcgggaacg
     4621 gccaccggag tctgagcacg atgtaagcgc gagggggcgc gggtagattg ggcatgaggc
     4681 cgagacagtt gtggcgcgcg gtggcgttct ctgccgcact gttcgccgcg cccgccccgc
     4741 gcgtcgaggc agacggggtg cgcgcgacaa ggtcgcccgc cccggcgcag tgccgtcgta
     4801 cagaccgcgc gatcagaaaa tctatcagga taggccacgg ctcgaccgtg gttagaaagg
     4861 acaccgtgcg gctctgcacg tcgcacgagt atcgcttcga cgcggatgcc ggggagaaga
     4921 taagcatcaa ccttgcgacc ggcaagcgca ccagtttcac gctccgaacg ccttcgggcg
     4981 acattgtcga gggcgcagac ggcgttaaga actggagcgg cgacgcccct gaggtgggcg
     5041 actacctcat taccatcggg acggacgcaa ctgccgccta cacgctctcg gtaacgctcg
     5101 gatagagcgt cgcccggacg actttcggtt aagaagttca ttgcaattat gtcgacagag
     5161 ttaatcaaag taacgatcaa tgggaacctc tacgaggtgg agaagggcgc gcggctgatt
     5221 gacgtgtgca gggagaaggg gcacgccgtt ccctctttct gttattacgc agacctcgcg
     5281 ctgcaagcct cttgccgcat gtgcctcgtg cgcatcgaga agatgccgaa gctgcaaacg
     5341 tcctgcacga tcacctgcac ggacgggatg agcgtcacca ccgagagcga cgagatcgag
     5401 aaggcgcagc gcgggatggt ggaattcctg ctcgccaatc acccgctcga ctgtcccgtg
     5461 tgtgatcgcg gcggcgagtg cgagttgcag gagatggtct tcgattgggg cggcctcgaa
     5521 gaacgctttc aggagaagaa gaattactac ccggaaaaat tcctctcgcc catcgtcgcc
     5581 aacgatccgc agcgttgcat tttatgtaaa cgctgcacgc gcgtctgcga cgagtggatg
     5641 ggcgaggacg cgattgaggc gggcgggcgc ggggctaaca ccgtcatcgg caccttcgac
     5701 ggctggctca actgttcgca gtgcggcaac tgcatcgaag tctgcccgac gggcacgctc
     5761 ctcgatgcga cctacaggca tcaagcgcga ccgtgggaac tctcgcagac gatcacgacc
     5821 tgcaacttct gctccgacgg ttgccagatg tcgctcggct cgcgcgcggg cgaagtgatg
     5881 cgttccgtcg cacgcgaccg ttacgtcaac ggcatcaacg gagagtttct ctgtatcaag
     5941 ggacgattcg cgcacccctt catcaatcac gaagagcgca tcaagacgcc gctcatccgt
     6001 tataagaagg gcgggaagct catccccgcg acgtgggacg aggccatccg ccacaccgcc
     6061 gcgaaactgc aagcgatcaa agacgcgcac ggcgcagacg cgctcggcgt cgtcggcagc
     6121 ccgcgcctca cgaacgaggc caactacgcg ctcgtcaagt tcgcacgcga agtcgtgggc
     6181 acgcagaact acacggcgac tgacgccttc tcgctcgcgc ccttcttcgc ttccctgagc
     6241 gcgccgctcg ccacgcaccg cgacattcgc cacgccaaga cgatcatcat catcggcggc
     6301 gagccggaag agttgcagcc gctcaccgga cgacagatca ggcaggccgt caggaacggc
     6361 ggcgcgaaac tcgtgatcgt caacagcgtg ccgattcgtc tgaaacaggc ggcgacgcag
     6421 ttcatacata tcaatcccgg cgcggaagat gccgccgtgc tcgctttcgc gggcagcgaa
     6481 tacgcgacgg cggcgcacaa gtcgggcatc gaggcgcagg agtttgaaga cgcgcggcgc
     6541 atcgtcgaag agacgacggg cgacgtggtc gtcatcttcg gcgagttgag cgcgacggcg
     6601 caaagcgcgc tggcaaacat ccctacgaac tacggcgaac gcgaaggccg acgcttcctg
     6661 ttccacccgc tgccgctctt caacaacagc ctcggcgtac acgacctcgg catgatgggc
     6721 ggcgcgctcg atgcaaatgc gatgctcgac gcggcaggca cgacgctgcg cgctctctac
     6781 gtcgcgggca gtttcctgcc gcaacacttc gccggacgcg ccgacgccct ctcgaagctg
     6841 gacttcctcg tcgtgcagga actcttcgag acggagacga ccgcgcacgc cgatgtcgtc
     6901 ttccccgccg cctcctacgc cgagacggac gggacgttta cgaacaacag cggcctcgtc
     6961 cagcgcgtgc ggcaatcaat tccgcccgtc caccagtcgc ggcctgactg gatgatcacg
     7021 gacgcgctcg ccgccgcgct cggcgtaaat ttcgacttcc agtggtcagc cggaaaagtc
     7081 ttcctcgaac tcgcgcgcaa cacgcaggct tacgcgggga tgaagtacgc gctactgaaa
     7141 gatgagtcgc agccggtgca ggcgcggcac gcggtcgcag gcgacaacgg cggcgcggcg
     7201 gacgtgctgg cggagttgcg cgaacgcgtc gaagcgttga gcgtcgaggg cgagaagatc
     7261 atggagacgc cgcccatcgg gcacgaactg ttcaagccgg gcacgctgac gggcaagacg
     7321 ccgcagttcc ccctgctcga cgcgggcaat ccgaaaccgc ccaccgtgct cgtctcgccg
     7381 ctctatcaga tttccgttga cccggccttg cgccgcgcaa ctgtcgttaa cgccgccgga
     7441 gactaatatg gagggatgaa ggatgaggga tgagggatga accagagaag ccctgaagct
     7501 gctcgatcat tccggttcat ccctcatccc tcatccttca tcccttaatt gttatggact
     7561 ggaacacact cacttacgtc ggcttgaaaa tgctgggcat caacatcgct ttcgtggtgg
     7621 tgttgatgat cgtggcctac acggtgctgg cggagcggcg cattctcgcg ctgattcaag
     7681 ggcggctcgg acccaaccgc gtcggctacg gcggcatcct gcaacctttc gcggatctcc
     7741 tgaagttcat cttcaaagaa gagatcgtcc cggacaaggc aacgaagttc gtctattttc
     7801 tcgcgcccat gatcgctatc gtcggcgcgt tgatgacgat catcgtgtat cccttcgggc
     7861 cggaagtgaa cctgcctttt atcggcacga tcaaactcgt cgtcacgcag tttgatgtcg
     7921 ggctgctcta cgtgctggcg atcacttcta tcggcgtcta cgggatcgcg ctcgccggtt
     7981 ggtcttccaa taacaagtac agcttgatgg gcggcctgcg cgcgtcggcg caactgatct
     8041 cttacgagtt gtcgctgggg cttggcctcg tgggcgtcgt gctgctctca ggcacgctcg
     8101 acctctacca gatcgtcgag cagcaggcgg gctactggtg ggtcggcggc gtcgtcccca
     8161 tcccgcgctg gaatattttc ccgcaactgc tcggcttcat cgtctacctg atctcggcga
     8221 ttgccgaaac gaaccgcgtg ccgttcgact tgccggaagc ggagacggaa ctcgtcgcgg
     8281 gctttcacac ggagtacagc gcgctcaagt tcgcgctctt cttcatggcc gagtacgtca
     8341 acatgttcac cgtctcgatg ctggcggcaa cgctcttctt cggcggctgg aatttcccgt
     8401 ggctcgacct cctggtggcg cgcggcggat tcgcgttcgc cttcctgagc ctgctcgcgt
     8461 ttctcgcgaa ggtgctcttc tttctctttt tgtacatctg gctacgcggg acgctgccgc
     8521 gttttcgctt cgaccagttg atgaacttcg gctggaaatt cctgttgccg gcggcgatcc
     8581 tgaacgtcgt cctgacggcg acaatcgttt tcgcgtttat gacttatcgc tcctgagcgc
     8641 gaaggatcga agacccggag cgagacgttt ttccggatta agccttcgcc ggattgtttc
     8701 caattatgac gactctactt tttgtgctgt tggcgggtct ggcgattggt tcggccatcg
     8761 cgatggtggt gcagcgcaac ccgctctaca gcgcgatctc tctgatcggc gtcttcatcg
     8821 cgctcgccgg actctatctg acgctcgctg ccccttttct cgcagccatc cagatcatcg
     8881 tctatgcggg cgcgatcatg gtgctcgtcg tcttcgtcat catgctcctg aacgtcgaag
     8941 aggaagagca taaacccttg cggctgggtt acctgaccgc cgttgcgatc ctgcttgcgg
     9001 cgatactctt cggcgaagcc ggattcatcc tctacttcat ggggcggtca ggcgtcgaac
     9061 cgctcgaccc gaacgccgtc ggcaacgcct cgaacgtcgg gctgaccgct tccatcggca
     9121 cggggctatt caccaactac cttttgccct tcgagatcac gtccgtactt ttgttgatgg
     9181 cgattgtcgg ggcgatgacg ctcgcgcggc gcgtcggcct gcgcccgacc gatctggcga
     9241 tccccggctc gcaggtgatc ccgcgccact ccatcgaacc gaaggccgtg ggcgacgcga
     9301 ccgaagtagt aagcacgacg accgccgacg cagaacgcgc gctcggcctc gaaggcgcgc
     9361 ggcacaggcg cgaagaatag gcgggggaaa caacttatgc ccgaagtctc tctttcatgg
     9421 tatctcgcgc tctcggcggt gttgttcacc atcggcgtgg cgggcgtcat catcaagcgc
     9481 aacgccatcg cgatgttcat gtgcatcgag ttgatgctga acgccgtcaa cttaactttc
     9541 gtcgccttct caagttattt ccgcgacgta agcggccaac tgttcgtctt catcgtgatg
     9601 acggtggcgg cggcggaagc ggcggtcgga ctcggtatta tcctcgccgt ctttcgcaac
     9661 cgcgaatcgc tcgacgtgga tgattcgagt gtgttgaaga attgagaata ccgaaggcag
     9721 aataccgaag gcagaatgca ggccgccgtc tcttcctgct gcattccgta ttctgaattc
     9781 tgactcctga aacttatgct gcgttacatc atccttgcgc cgctcgtcgg cgcggctatc
     9841 aactggctgg tcggtcgccg cgtgcggagc gaagcgttta tcggtgcggt cgcgagtttg
     9901 tccatcgccg tctcgtgcgc gttgtcgctg tggctcgcct tcggaccaaa cggcgcgctc
     9961 gtgggcgaac cgaaggtcat cctcgatcac gtctggacat ggatggaggc cgggacgttt
    10021 cgcgccgaca tcggcttcgc gatggatcgc ttgagcggca tctatgcctg cttcatcacg
    10081 tttgtcgggc tgctcataca cgtcttcgcc accggctaca tgcacggcga taagagcttt
    10141 tatcgcttct tcgcctacct gaacctcttc atgttcatga tgctgacgct ggtgctggcc
    10201 gacaacctgc tcctgatgtt cgtcggctgg gaaggcgtcg ggctgtgctc ctatcttttg
    10261 atcggctact acattgaccg gcgggaagcg ggcgacgcgg cgaagaaagc ctttgtcgcc
    10321 aaccgcgtgg gcgactgggg cgtcgtgctc ggcatcatgc tcgtcttcgc catcacgcag
    10381 gccgcgggtc acgcttcgat cagtttcttc gacaagcccg gcgtcgtgag cgcgttgaag
    10441 accatcaacg cgatgggcgt cgaagccttc tctccgggca cgctcatcgc gggcggcatc
    10501 acgtccgccg cgctcctgct ctttatcggc gcgacgggca agagcgcgca gatacctctg
    10561 ttcgtctggc tgcccgacgc gatggc
//
LOCUS       DASWNS010000033         8968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_260717, whole genome shotgun sequence.
ACCESSION   DASWNS010000033 DASWNS010000000
VERSION     DASWNS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260717"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..530)
                     /locus_tag="VGB76_00970"
     CDS             complement(192..530)
                     /locus_tag="VGB76_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097497.1"
                     /translation="MTGSRNDEADRRAGGTGGTQAGNGENEDLFPGEIGGAQTYLPSD
                     GRDAGEGTPAVSANNTTGAVGEMDFTGAPAGTSGGGDAGAGSSEGASVGGGTGGGAMT
                     TSGQTGVDKE"
     gene            754..1197
                     /locus_tag="VGB76_00975"
     CDS             754..1197
                     /locus_tag="VGB76_00975"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0097498.1"
                     /translation="MKTTPTQTQISAGGVVFRRGEARIEVALISVGDKNRWQLPKGLV
                     DAGEDAETAAVREAREETGLETELVAPLDKIEYWYYATKRGQRVRFHKFVYFYLLRYL
                     SGDVRDHDAEVNEARWLEIDEALALLAFAGERRVVERARELIAAG"
     gene            complement(1249..1473)
                     /locus_tag="VGB76_00980"
     CDS             complement(1249..1473)
                     /locus_tag="VGB76_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097499.1"
                     /translation="MTNKSRGKVNQPPTGEGKKQSAESGAENKLGKRSEVVQAQRDRP
                     DENLGTDADEAAAQPKSGAKGNPKRPKAGR"
     gene            complement(1525..2073)
                     /locus_tag="VGB76_00985"
     CDS             complement(1525..2073)
                     /locus_tag="VGB76_00985"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0097500.1"
                     /translation="MKERKHGAWTIKETTEKYRDEFIAVYEDSVIRPDGKPGTYATVQ
                     LKPGVSVLPFEPDGTIYLTRQFRYATGAESIEVVSGGIDEEDASGAALKELREELGIE
                     AAELISLGRMDMDTSIVRSPVSLFLARGLKFTEAEQEGTERIELFKTTLEQAVGMVLR
                     SEITHSPSCVLLLKAGRYLEGE"
     gene            complement(2070..2948)
                     /locus_tag="VGB76_00990"
     CDS             complement(2070..2948)
                     /locus_tag="VGB76_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEY0097501.1"
                     /translation="MKQQEFLVPAGARLRLKNYDPAFTGKFKSEAEAEAKIRDDCENL
                     ARYQDKLLAEERQSLLLIFQAMDGAGKDGTIKHVMSSVDPQGCVVMLSEKPGATELKH
                     DYLWRFVRYLPKRGQIGIFNRSYYEEVLSTRVHPERLEDQQLPPRIRDAKNIWRQRFT
                     QINNFEQYLTENGVHVMKFFLHLSKEKQRERLLERLDVPEKKWKFSMNDVAERAHWDE
                     YMIAFEDAITQTNKRHAPWYVVPADHRWFSALVVADLVLGKLKSLKLRYPPASSDGKK
                     EMEKARKLLEREEEKS"
     gene            complement(3050..4387)
                     /locus_tag="VGB76_00995"
     CDS             complement(3050..4387)
                     /locus_tag="VGB76_00995"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0097502.1"
                     /translation="MEVIQAGLTREGVPETPTPEKFRLPAALRSLGHKNYRLFYGGQF
                     ISLTGTWMQMVAQSWLVYRLTGSAATLGFVAFAGQVPGFLLAPLGGTLADSLSRHRIL
                     IATQTLAMLLALALAVLTLTGSVAVWHVYVLAALLGMVNAFDIPARQAFVADLVSRDD
                     LVNAIALNSSMINGARLVGPAMAGVLVATIGEGWCFFVNALSYVAVIGGMLRMRVAKR
                     EPDATTEARRSPIRRVVEGFSFVARTAPIRALIILLGLVSLLGMPYTVLMPVFADRVL
                     HVGASGLGVLLGAAGVGALAAAILLLTRRGVRGMGRWVAFSSAGFGASLILFSQVQVF
                     WLAVVVLVCVGFSMIIQIASSNTLVQAMVPNELRGRVMAVYSMMFLGMAPLGALLAGW
                     LAERMGAANTVAIGGAGCILSALVFSLRLPALRREARTLLGVETGGGVVEAGR"
     gene            complement(4585..5403)
                     /locus_tag="VGB76_01000"
     CDS             complement(4585..5403)
                     /locus_tag="VGB76_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEY0097503.1"
                     /translation="MSHKTDGKRASTAHVERASTTRDERVERTRGNVWRRIALFVLAC
                     AYVILWVGGVGHYLFVGAVAAEQSWLASAFLSVAGLIVLLAATSRRAALQLVAVAALG
                     LGFELCGVRYGLPFGLYAYTGVLQPTIFGVPLVMAFAWMSLVAYLKQSLSRLPLKPWV
                     GALIASAWMTTIDLVIDPLAANQLGYWRWVERGSYYGIPSSNFVGWFVCSLSIFLLFR
                     QKWQPNFWHRLTGASIILFFTLVALSFQLYLAALFGFGLCAAELLIHLRRKIAS"
     gene            complement(5400..6872)
                     /gene="crtI"
                     /locus_tag="VGB76_01005"
     CDS             complement(5400..6872)
                     /gene="crtI"
                     /locus_tag="VGB76_01005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEY0097504.1"
                     /translation="MKRKNAIIIGGGLGGLATALRLSAAGWHVVVCEQGTTLGGKMNR
                     WQERGFRFDTGPSLITMRWVFEELFAAVGSSMDEHLELVSMHPLAEYVYDDGTRFKYS
                     SNLPGWLATLRELEPRDVDGFFRFMNLGARLLEVSQETFFRRRPLDAPDLRSLRAMRQ
                     LPLRHAWGNYHRTVAAHFKSPHLRQLFDRYPTYVGSSPYSSPATLAVIPYLEYAYGGW
                     HIKGGLYQLVASLVALAKRRGVTLRTCARVERIEHDGRRVNGVLLANGLRLMADAVVM
                     NGDASMTNAMLGLKDAATLASKERSLSGLVFLAGVKRTHAELPHHAVYFSSDYKREFA
                     QLFDERRFPDDPTVYVSAPSRSDRSLVPHGEGETLFIMANAPANDGDEWTEAGIGQAR
                     ARVFARLQKGGFPAIESELVVGDVWTPGRLRERYSMPGGAIYGTHSHGWRRAFLRPPN
                     KDTRFAGLYYVGGSTHPGGGTPTVLLSAKITSELVRRCER"
     gene            complement(7002..8162)
                     /locus_tag="VGB76_01010"
     CDS             complement(7002..8162)
                     /locus_tag="VGB76_01010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0097505.1"
                     /translation="MIFFYLLLIPAAALLFVAVWNALAWPEVAAEGSVAPEVAEGAAA
                     RVSILIPARNEETNLPACLDAALAQGEIVRELLVYDDHSTDATSEIIEQYAQRDARVR
                     SVPAETLPAGWCGKTFACAQLAASARGDWLLFLDADARLGAGAASRMLAEAERRRLTL
                     LSCWPALELSSFWERALMPLLNFVVFTLYPAPLALRRADASLGLAHGACLLAHRSTYA
                     LVGGHGAVRDQLFEDVRLAQLWRARGQASLCLDGQGIVSVRMYRSFAEIWRGFQKNFF
                     PAFRRETSFWLFMSLHLTLFLLPSILAPFVLTRGAVFGGDAATLILLLLFCVLAMRAA
                     LALRFKHPWWSVLLHPCGEAILLALGLSSWWRCKSGRGVVWKGRRYRAGAEV"
     gene            complement(8192..>8968)
                     /locus_tag="VGB76_01015"
     CDS             complement(8192..>8968)
                     /locus_tag="VGB76_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0097506.1"
                     /translation="ALSAARLAGATHEQGIIVGCAVEFLHSSSLILDDLPAMDDADLR
                     RNRRALHLVYGEGLAVLAAVALLNQAYALLTRAASAAACVKAGTTEALLGEAAHAVGA
                     DGMIGGQVIDLELQPGTAGERALASRDLKTVALMRLMMVSGALARGADDETITTLAKF
                     GECFGRVYQICDDLLDEPAESARSGKTAGQDARHLRPSAVSVLGIDASQSLAFALVRE
                     GTSLLVRKFGERNDARVLVEAAQLMLRKVKLAGVEQLSVA"
BASE COUNT         1743 a         2924 c         2710 g         1591 t
ORIGIN      
        1 aagaataagg agatgaagaa agacgagtga agcgtacata gataaaagcc gtgaatcgtg
       61 aatcgtaaat agataaaaga tgtaaatcgt caatcgtcaa tcgtaaatag actaagacag
      121 gctttctctc ttcacgattg acgattcacg attcgcagct tttgaatctc ttcacgattc
      181 acggctttca ttcactcttt atccacgccc gtctggccgc tggtcgtcat tgcgccgccg
      241 cctgtgccgc cgccgaccga tgcgccctcg ctgctgcccg cgcccgcatc accgccgccg
      301 ctcgtgcccg cgggtgcgcc cgtgaagtcc atctcgccga ccgcgccggt ggtgttgttg
      361 gcacttacag ccggcgtgcc ctcgcctgcg tcgcgcccgt ccgaaggaag gtaggtctgc
      421 gcgccgccga tctcgccggg gaacaggtct tcgttctctc cgttgcccgc ctgcgtgccg
      481 ccggtgccac ccgcgcgcct gtccgcttca tcatttctgc tgccagtcat acttttacat
      541 ccttccttaa gctgctcgaa attggtgaac atggcgaatg gagctttggc ggtgcagcgc
      601 gcaccgaaaa cttctcgccc gttcgaacaa aatgttatta gtggaacgcg gcgagtgtat
      661 ggcgcgcggg cgcgcgggtc tgtaccgtgg cgtactaagc gacgtgcggc ctcgttgccg
      721 cagttcagcc cgtcgcatct ataatctttc gacatgaaga cgacgcctac acagacgcaa
      781 atctcagccg ggggcgtcgt cttccgtcga ggcgaggcgc gcatcgaagt ggctctgatt
      841 tcggtcggcg acaaaaatcg ctggcagttg ccgaagggct tggttgacgc gggcgaggac
      901 gcggagacgg ctgccgtgcg cgaggcgcgc gaggagacgg gcttggagac tgaactcgtc
      961 gcgcccctgg acaagatcga gtactggtac tacgcgacga aacgcgggca gcgcgtgcgc
     1021 ttccacaagt tcgtctattt ttacctgctg cgctacctgt ccggcgacgt gcgcgaccac
     1081 gacgcggaag tcaacgaggc gcgctggctg gagatagacg aagccctcgc gctgctggcc
     1141 ttcgcgggcg agcgcagagt ggtcgagcgt gcgcgcgagt tgatcgcggc aggctgagtc
     1201 cggcgttaaa gaaagtcgtt cgtgcgcagg tgcgtcgagg ctcaggcgtc agcgtccggc
     1261 cttcggtcgc ttcggattgc ccttcgcgcc ggacttaggc tgcgccgccg cttcgtcggc
     1321 atccgtcccc agattttcgt ccggcctgtc gcgttgcgcc tgcaccacct cggaacgctt
     1381 gccgagtttg ttctccgctc cgctctccgc gctctgcttc ttgccttcgc ccgtcggcgg
     1441 ctggttcact ttgccacggc tcttattcgt catttccctc tcctccctta atcgtctctc
     1501 ccggccacat tatcgccgct gcccctactc tccttcaaga taacgcccgg ccttcaagag
     1561 cagcacgcaa ctgggactgt gcgtgatctc gctccgcaag accatcccga ccgcctgctc
     1621 cagcgtcgtc ttgaacagct cgatacgctc cgtgccttcc tgttccgctt ccgtaaactt
     1681 caacccgcgc gcgaggaaga gactgacggg cgagcgcacg atggaggtgt ccatgtccat
     1741 ccgccccaga ctgatcaact ccgccgcttc gatgcccaac tcctcgcgca gctctttcaa
     1801 cgccgcaccc gacgcgtcct cttcgtcaat cccgccgctc accacttcaa tgctctccgc
     1861 gcccgtggca taacgaaact ggcgcgtcag gtagatcgtc ccgtcgggtt caaaggggag
     1921 caccgagacg cccggcttca actgcaccgt agcatacgtg cccggcttgc cgtccggcct
     1981 gatcacgctg tcttcataga ccgcgatgaa ctcgtctcga tacttctccg tcgtctcttt
     2041 aatcgtccac gcgccgtgct ttcgctcctt caacttttct cctcctcgcg ttcgagcagt
     2101 ttgcgcgctt tctccatctc ctttttgccg tcgctgctgg caggcgggta gcgaagtttc
     2161 agagacttca acttgccgag cacgaggtct gcgacgacga gcgcgctgaa ccagcgatgg
     2221 tctgcgggaa cgacgtacca gggcgcgtgc cgcttgttcg tctgcgtgat ggcgtcttca
     2281 aacgcgatca tgtattcatc ccaatgggcg cgctccgcca cgtcgttcat ggagaacttc
     2341 catttcttct ccggcacgtc gaggcgttcg agcagacgct cgcgctgctt ctctttcgag
     2401 aggtgcagga aaaacttcat gacgtgaacg ccattctccg tcaggtattg ctcgaagttg
     2461 ttgatctgcg tgaagcgttg ccgccagata ttcttcgcgt cgcggatgcg cggcggcaac
     2521 tgctggtctt cgagtcgttc ggggtgaacg cgcgtcgaga gcacttcctc gtagtaggag
     2581 cggttgaaga tgccgatctg cccgcgcttg ggcagatacc tgacgaaacg ccagaggtaa
     2641 tcgtgcttga gttccgtcgc gccgggcttc tcgctgagca tcaccacgca cccttgtggg
     2701 tctacgctcg acatgacgtg cttgatcgtg ccgtccttgc ccgcgccgtc catcgcctga
     2761 aagatgagca ggagcgactg ccgttcctcc gccagaagtt tgtcctgata cctcgcgagg
     2821 ttctcgcagt cgtcccgaat cttggcttcc gcctccgcct cgctcttaaa ctttccggtg
     2881 aacgccggat cgtaattctt cagacgcaat ctcgcgcccg caggcacgag aaattcctgt
     2941 tgcttcattc acgctccacc cttcgtttct ttcgactcaa aaacagagac gcacgttgac
     3001 agtttgccca gacatcgtgc gcccgaatca ttcccgcacc atcttgcaat taacgccccg
     3061 cctccacaac tcccccgcct gtctccacgc cgagcagcgt gcgcgcctca cgcctgagcg
     3121 cgggcaggcg caggctgaag acgagcgcgc tcaggatgca acccgcgccg ccgatggcga
     3181 ccgtgttggc cgcgcccatg cgctccgcga gccagcccgc cagcagcgcg ccgagcggag
     3241 ccatgccgag aaacatcatc gaatagaccg ccatcacgcg cccgcgcagt tcgttcggaa
     3301 ccatcgcctg cacgagcgtg ttcgacgaag cgatctgaat gatcatcgaa aagccgacgc
     3361 agaccagcac gaccacggcc agccagaaga cctgcacctg cgaaaacagg atcaggctcg
     3421 cgccgaatcc cgccgacgaa aaggccaccc aacgccccat cccgcgcacg ccccgccgcg
     3481 tgagcagaag gatcgccgcc gcgagcgcgc ccacgcccgc cgcgccgagc aacacgccga
     3541 gtccgctcgc gccgacgtgc agcacgcggt cggcaaagac cggcatcagc accgtgtaag
     3601 gcatccccag caaactcacg aggccgagca agatgatcaa agcgcgaatc ggagccgtgc
     3661 gcgcgacgaa gctgaaacct tctaccacgc gcctgatcgg cgaacgtcgt gcttccgtcg
     3721 tcgcgtccgg ctctcgcttc gccacgcgca tgcgcagcat tccgccgatc acggccacgt
     3781 agctcaaggc gttgacgaag aagcaccagc cctcgccgat ggtggcgacg agcacgcccg
     3841 ccatcgccgg gccgacgagc cgcgccccgt tgatcatcga agagttgagc gcgatagcgt
     3901 tgacgagatc gtcgcggctg acgaggtctg cgacaaacgc ctgccgcgca ggaatgtcga
     3961 acgcgttgac catgcccaga agcgcggcga gcacatagac gtgccagacg gcgacgcttc
     4021 cggtcagcgt cagcacggcg agcgcgagcg cgagcagcat ggcgagcgtc tgcgtcgcga
     4081 tcaggatgcg gtggcggctg aggctatcgg ccaacgtgcc gcccagaggc gcgagcaaaa
     4141 atccgggcac ctgccccgcg aacgcgacga agccgagcgt cgccgccgaa cccgtcaagc
     4201 gatagacgag ccacgactgc gccaccatct gcatccacgt gcccgtgagc gagatgaact
     4261 gcccgccgta gaagaggcga taatttttgt ggccgagcga gcgcaaggcc gccgggagcc
     4321 tgaatttttc cggcgtgggc gtctccggca cgccctctct cgtgagtcct gcttgaataa
     4381 cttccacccc ttgcccgtat cgcctgagtc tccacggcgg cgcacaaacg cgcacgcaac
     4441 atctcaggcc gacactcttt taaacagatc gtcttagata gatgaactac tgccactata
     4501 ggatcgacgc gggacagaat gatgaccttc atttcaagaa actgtgacaa aagagaagac
     4561 ccgccacggc ttcgagagaa tatgctatga agcgatctta cgtctcaggt ggatgagcaa
     4621 ctctgccgcg cataatccga agccgaagag ggcggcgagg tacagttgaa atgagagcgc
     4681 gacgagcgtg aagaacagaa tgatgctcgc gcccgtgagg cgatgccaga agttcggctg
     4741 ccacttctgc cggaacagga ggaagatcga gagactacag acaaaccagc ccacgaaatt
     4801 actcgacgga atcccgtagt agctgccccg ttcaacccaa cgccagtagc cgagttgatt
     4861 tgcggccaga gggtctatca caagatcgat ggtcgtcatc cacgcggaag cgatgagcgc
     4921 gccgacccac ggcttgagcg gcagacgcga gagcgactgc ttcaaatagg cgacgagcga
     4981 catccacgcg aaggccatca cgagcggtac gccgaagatc gtcggctgca acacgcccgt
     5041 ataagcgtaa agcccgaacg gcagaccata gcgcacgccg cacaactcga agccgagtcc
     5101 gagcgcggcc actgcgacga gttggagagc cgcgcggcgt gaagttgccg cgagcagaac
     5161 gatgagtccg gccacggaaa gaaaagcgga ggcgagccag ctctgttcgg cggcgaccgc
     5221 gcccacgaac aggtaatgcc ccaccccgcc tacccacaag atcacatatg cgcacgcgag
     5281 cacgaagagc gcgattcgac gccacacgtt acctcgcgtc ctctccacac gttcgtcacg
     5341 cgtcgtgctc gcgcgttcaa cgtgcgccgt gctggcgcgc ttgccgtctg tcttgtggct
     5401 catctctcgc atcgtcggac gagttcggaa gtgattttgg cggagagcag aacggtgggc
     5461 gtgccgccgc cgggatgggt gctgccgccg acgtagtaca gacctgcgaa gcgcgtgtct
     5521 ttgttcggcg gtctgaggaa agcccgacgc cagccgtgcg aatgcgtgcc gtagatcgcg
     5581 ccgccgggca tcgaataacg ctcgcgaagg cggcccggcg tccatacatc cccgacgaca
     5641 agctccgatt cgatggcagg gaagcccccc ttttgcaggc gcgcgaagac gcgtgcgcgt
     5701 gcctgcccga tcccggcctc cgtccattcg tcgccgtcgt tcgccggagc gttcgccatg
     5761 atgaaaagcg tctcgccctc gccgtgcggc acgagcgagc ggtcgctgcg gctcggcgcg
     5821 ctcacataga cggtcgggtc gtcgggaaat cgccgttcgt cgaaaagttg tgcaaactcg
     5881 cgcttgtagt cgcttgaaaa atagaccgcg tgatgcggca actccgcgtg cgtgcgcttg
     5941 acgccagcga ggaagacgag gccggagagc gaacgctcct tcgacgctaa cgtggcggca
     6001 tctttcaacc cgagcatcgc gttcgtcatc gacgcgtcgc cgttcatcac caccgcgtcg
     6061 gccatgagcc gcaggccgtt tgccagcagc acgccgttga ctctgcgccc gtcgtgctcg
     6121 atgcgctcga cccgcgcaca cgtcctgagc gtcacgcccc tgcgtttggc gagtgcgacg
     6181 aggctggcga cgagttgata gaggccgccc ttgatgtgcc agccgccgta ggcgtattcg
     6241 aggtaaggaa tgacggcgag cgtcgcgggt gaactgtatg gcgacgagcc gacgtaagtg
     6301 ggatagcggt cgaagagttg gcgcaggtgc gggcttttga aatgagccgc gacggtgcgg
     6361 tggtagttgc cccacgcatg gcggagcggc agttggcgca tggcacggag cgaacgcagg
     6421 tcgggcgcat cgagcgggcg gcggcgaaag aaagtctcct gcgagacttc aaggagacgc
     6481 gcgccgagat tcataaaacg aaagaatccg tccacgtcgc gcggctcaag ctcgcggagg
     6541 gttgccagcc agccgggcag gttggaactg tacttgaagc gcgtgccgtc gtcgtagaca
     6601 tattctgcga gcgggtgcat ggagacgagt tcgaggtgtt cgtccatcga actgccgacc
     6661 gccgcgaaca gttcttcaaa gacccaacgc atcgtgatga gcgaagggcc tgtgtcgaaa
     6721 cgaaagccgc gctcctgcca gcggttcatc ttgccgccga gcgtggtccc ctgctcgcag
     6781 acgacgacgt gccagcccgc agccgacagg cgcaacgccg tcgccaatcc gccgaggccg
     6841 ccgccgatga tgatcgcgtt ctttcgtttc ataagcgact gccgggcgaa agtttcaagt
     6901 ctcaagtttc aaggctcagg ttagaagtct caagttacaa gtcgaagaca agttttcgac
     6961 ttgagactta agactttctc tgcggctctg cgtcgtgcgt tctaaacttc cgcgcccgca
     7021 cggtaacgtc tgcctttcca tacgacgccg cgcccgctct tgcacctcca ccatgaggag
     7081 aggccgagcg cgagcaggat cgcttcaccg cacgggtgca gcaagactga ccaccacgga
     7141 tgtttgaaac gcagagccaa tgccgcacgc atcgcgagca cgcagaagag taagaggaga
     7201 atcagcgtgg ccgcgtcgcc gccaaacact gcgccgcgtg tcaacacgaa cggcgcgagg
     7261 atagacggga gcagaaacaa cgtcaggtga agcgacatga agagccagaa actcgtctcg
     7321 cggcggaagg cgggaaagaa gttcttctga aagccccgcc agatttcagc gaaactccgg
     7381 tacatccgca cgctcacgat tccctgcccg tccagacaca gactcgcctg accgcgtgcg
     7441 cgccacaact gcgcgagtcg cacgtcttca aagagttgat cgcgcaccgc gccgtgtccg
     7501 ccgacgagcg cataggtcga acgatgcgcg agcaggcagg ctccgtgcgc gaggccgagc
     7561 gaagcatccg cgcgccggag cgcgagcggc gcgggataga gcgtgaagac gacgaagttg
     7621 agcaaaggca tcaaagcccg ctcccaaaaa ctacttagtt cgagcgcagg ccagcaggag
     7681 agcagggtga ggcggcgacg ctccgcttcg gcgagcatcc gcgacgccgc gcccgcaccg
     7741 agccgcgcat ccgcgtcgag aaagagcagc cagtcgcccc gtgcgcttgc ggccaactgc
     7801 gcgcaggcga aagtcttgcc gcaccaacct gcgggcagag tctctgcggg cacgcttctc
     7861 acacgcgcgt cgcgctgtgc gtactgctcg atgatctcgg aagtagcgtc cgtcgagtgg
     7921 tcgtcgtaga cgagcagttc gcgtacgatc tcgccttgcg cgagcgcggc gtcgaggcag
     7981 gcgggaaggt tcgtctcctc gttgcgcgcc ggaatcagga tggagacgcg cgccgctgcg
     8041 ccctccgcga cctccggcgc gacgcttccc tccgccgcca cctccggcca cgcgagcgcg
     8101 ttccacacgg cgacgaagag caaggccgcc gcggggatga gcaagaggta gaaaaagatc
     8161 atgcgccggg aagttgctct aaaaagtcgt gtcaggcgac ggagagttgt tccacgcccg
     8221 cgagtttcac tttacggagc atcaactgcg ccgcctccac cagcacgcgc gcatcattgc
     8281 gttcgccgaa ttttctgaca aggagcgacg tgccctctcg caccaacgcg aacgccagac
     8341 tttgcgacgc gtcaatgccg agcacggaga cggctgacgg gcgcaagtgt cgcgcgtcct
     8401 gccctgcggt cttgcccgaa cgcgcgctct ctgcgggttc gtccagcagg tcgtcgcaaa
     8461 tctgatagac gcgcccgaag cactcgccaa actttgcgag cgtcgtgatc gtctcgtcgt
     8521 ctgcgccgcg cgcgagcgca ccggacacca tcatcaagcg catcagcgcg acggtcttca
     8581 agtcgcggct ggcgagcgcg cgttcgccag ccgtgcccgg ttgcaactcc aaatctatca
     8641 cctgcccgcc gatcatcccg tccgcgccga ccgcgtgcgc ggcttctccg agcaacgctt
     8701 cggtcgtgcc cgctttgacg cacgctgccg cagaggcggc gcgcgtcagg agcgcgtaag
     8761 cctgattgag caacgcgacg gcggcgagca cggccagccc ctcgccgtag acgagatgca
     8821 gggcgcgacg attgcgacgc aaatccgcgt cgtccatcgc gggcagatcg tcgaggatga
     8881 gagaactgct gtggagaaat tcgacggcgc aaccgacgat gatgccctgt tcgtgtgtcg
     8941 cccctgcgag tcgcgcggcg gagagcgc
//
LOCUS       DASWNS010000034        32060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_1294, whole genome shotgun sequence.
ACCESSION   DASWNS010000034 DASWNS010000000
VERSION     DASWNS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 32060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32060
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VGB76_01020"
     CDS             <1..410
                     /locus_tag="VGB76_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0097507.1"
                     /translation="PFTINYLGKDPADDPEIYQKTSPMSYIKSARTPTLIQHGELDRR
                     VPIANAYELRQGLEDRGVRAEMIVYKGFGHGITKPRAMRAVMHHNLAWFNHYLWNEPL
                     PDLANLPLPKKEEKDGEKKDEEKKTAAAAAATN"
     gene            542..1939
                     /locus_tag="VGB76_01025"
     CDS             542..1939
                     /locus_tag="VGB76_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0097508.1"
                     /translation="MRRIAPFLLCLCFITTGQHLTASAQNWPQFRGAQASGVAQSTQK
                     TPVSWNGEKSVGVRWKTPIPGIGQSSPVVWGNRVYLTTAVSAEAKPAARYGLYGDVEP
                     VKDEPKHTWHVMALDKTTGKVVWDRTTLEGLPKTKRHPKGTHASSTPATDGKHVVALF
                     GSQGVLACYDTNGKLLWKQEMGVLDAGWFYDPDYQWGHASSPVIYKNLVIVQADIQKN
                     SFLAAYDLKTGKQVWKTVRDEIPSWGTPTVYEGKTRPELIANATKFIRGYDPLTGKEL
                     WRLGGNSEITTPTPIIAHDLIYVTAGYRPIQPIYAIRLGANGDITLKEKQESNEFVAW
                     SKQRGGPYMPTPIVYGDYFYTCSNNGVVTAYNAKTGERVYQERIAGKGGAFTASPVAA
                     DDKLYFSSEDGEVFVVKAGPTHELLATNPMGEPLMATPAISDGMVIVRGQHHLFGIGE
                     GAPAPTMKSKAKAAK"
     gene            2205..3563
                     /locus_tag="VGB76_01030"
     CDS             2205..3563
                     /locus_tag="VGB76_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0097509.1"
                     /translation="MKRFFAGLLLSTFVCLVAVSPLSSVAVKAKGGNWPQWRGPEGQG
                     VSMETGLPTEWSATKNIKWKTAIAGRGHSSPIVWGKKIFLTTALDGAPIPGAKPGVTH
                     KMADGSDFVHPDAVGADLKHTFKVICVDRETGKILWERVAYEGVVYDSRHKKASFASS
                     TPATDGKYVFAFFGAEGLYAYDMGGKLLWKQNLGKLGTASVGYGVSPILYGNLVIMQC
                     DDSGMNSFIAAFDKKTGKEMWRAARKVDLTWSTPVLVNTGKRTELIASAAEAIIAYDP
                     QTGKELWRHKGLESNAVNTPVVSQDMVVVTSGYPAKIALALKAGGSGDITGTPQLIWK
                     YNKGTAYVPSPILYGEYLYLMTGNGSITCLEAKTGKVVYEAGRVPKPTSFMASPVAYE
                     GKILLTSEEGDTYVLKAGPKHEVIRTNTLGEPVYASPAIADEKLFIRGEKNLYCITNA
                     RG"
     gene            3777..5300
                     /locus_tag="VGB76_01035"
     CDS             3777..5300
                     /locus_tag="VGB76_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0097510.1"
                     /translation="MFESQDTTPWYRSRSTIIGVSVCCAALVLTLFYHFYGYRYFTNR
                     DAHYAELERHRAQQAALAGNEPPPAAPAGQPTAPEQNAPTTGQPTSAPGATVAEASGN
                     SATANGQDANHPVAPNASSARNYWTNFRGLNRDGRYEEGAVKVDWPAGGLKPMWKQPV
                     GGGFSSFVVADGTAYTIEQRRKQEVAVAYNVETGRELWAHGWNAEFSPDGTGDGPRST
                     PTWDGGRLYALGAAGELRALDAKTGRLFWQKNILADNGASNIQWGMAASPLIVDDKVV
                     VLAGGTSGKGVVAYNKLTGARVWSSLNDRASYTSPMLVTLAGKRQIMVVTASRIAGLD
                     PADGAVLWTHPWDVSMGINVSQPLITGANRFFISAGYGKGATLVELQGSGKSFTTKSI
                     WENINLKTKFNSPVVHNGHAYGLDEGILTCLDLATGERKWKGGRYGYGQVLLASNHLI
                     VTTDTGDLVLVKADPAAYAEVAKFNALSGKTWNVPALAGGRLLMRNGNEMAAYNLTD"
     gene            complement(5421..5606)
                     /locus_tag="VGB76_01040"
     CDS             complement(5421..5606)
                     /locus_tag="VGB76_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097511.1"
                     /translation="MADDKEREYEKTPTAGKGGSPASSTPNEEARGAGEDDEASASAR
                     TPKESKESAGGKGGGLH"
     gene            complement(5689..8871)
                     /locus_tag="VGB76_01045"
     CDS             complement(5689..8871)
                     /locus_tag="VGB76_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0097512.1"
                     /translation="MKSKITFLSFLLVLLIAPATFAQTHRASIRGTVSDPNRAVVPGA
                     TVTLTNRATNEIRTVTSNASGEYAILSLAPGVYLVAVEAAGFQRLPQEVALQVNQELR
                     LDAALSVTDVTITVDGIISEPAPLRKDSAALGTVIAERQITELPLDGRNFLELALLAP
                     GTAPSAPGSAGSVRGDFAFSANGAREDSNNFLLDGVYNLDPKLNSFGVRPPVDGIQEF
                     EISTSTYDASFGRNAGAQVNVISKSGSNVLHGTLYEFHRNAAFDARNFFAPADQPDPK
                     YIRNQFGFSLGGAIVKDRTFFFGDYEGTRTREGITRITNVPTLAERNGDFSQSLFARP
                     FNPFVQQPFTNGVIPVEFQNPIGRAIANLYPLPNRNVPFANFVSSPTLRDRNDNFDAR
                     IDHRFSDTSTFTARYSFGDRNLFDPFTGTTFSLVPGFGNFVPRRSQNLMLSETHVFSP
                     SVVNEARFAFSRVASAVTSEGAGASLNRQVGLPELSNNPRDFGLSFITITGYSPLGDE
                     FNNPQQSATNVFQFLDNLSYVRGAHLAKFGFEFRAMQQNAFRDVQSRGFLTFSERFPL
                     TGNALADLLLGFPALTGGARLDNHQHLRGESYAVYANDSFRITPRLTISAGLRYEYNS
                     PPVDVEDRANLYDPATRTLSRVGTNGIPRGGYAPDRNNFAPRLGLAYTLNESGTTVVR
                     GGYGVYYDQPALAPGEGLYFNAPYFDFNLYFSLEGLPLTVNNPFPSNFPVPLPDTALS
                     FQRDLRTAYMQHWNLNLQQQLGQNRVLEIGYAGSKGTKLLTARDINQPRPSTLPFVLR
                     PVPQFDDINLIESRGNSNYHSLQVRLQQRLTNGLALLSSYTYAKSLDDASNFFTSAGD
                     PNFPQDSLNLRAEHGRSNFDVRHRLSTSFSYELPFGHGRKFLSDEGFLTTLLSGFQFN
                     GIVTLQTGRPFTVALLSEIDNSNTGRSTLGFGANDRPNVAGDPNVANRTPERWFNTQA
                     FVFPAFGTFGNSGRNTLDGPGYQNVNASLLKNTRLGERVNLQLRLEAFNLFNHPNFNL
                     PDNFLGSPTFGQILSARDPRHLQFGAKLLF"
     gene            complement(9144..9593)
                     /locus_tag="VGB76_01050"
                     /pseudo
     CDS             complement(9144..9593)
                     /locus_tag="VGB76_01050"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(9991..10119)
                     /locus_tag="VGB76_01055"
     CDS             complement(9991..10119)
                     /locus_tag="VGB76_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097513.1"
                     /translation="MENLLDKAAATDTGDTLIVLSNYDPPAVMPLVREIEQFVTKD"
     gene            complement(10256..11536)
                     /locus_tag="VGB76_01060"
     CDS             complement(10256..11536)
                     /locus_tag="VGB76_01060"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0097514.1"
                     /translation="MHPTLSRILRPTTRRTHPRLQNSSSRALFDAPRTRAASLCLPAL
                     CFIVALASFNAAQQTATTQPPRQPEQPDMTIDAGVRTQVIEGALKNLHEAYVYPETAK
                     RMEQAIRERVAKKEYEEVTSAKALAQMLTQHLQAVSRDKHLRVNYRAEALAEGEALPG
                     ARIVRRRPGDDGGGGGNPASGERVVRRNPNDLTGNMGLEKVETLEGNLGLLEFSYFDA
                     SGEARQKISDAMNRLADTDALIIDLRRCRGGASAAITLLMSYFFDKSIHLSDAYDRVM
                     DETISFWSLAEVPGRRYGRKDVYILTSSFTFSAAEDISYTLKNLKRATIVGETTGGGA
                     HPVMGKRLSDHFFVMVPFARSISPITKTNWEGTGVEPDIKTPARHAFKVAQLAALKKL
                     SAQRQDAKASAQLKSLIETLQKELDELKKESSRS"
     gene            11773..12399
                     /locus_tag="VGB76_01065"
     CDS             11773..12399
                     /locus_tag="VGB76_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0097515.1"
                     /translation="MFCPQCGANQSEELKFCNLCGANLQAVRQVVALRETSEDKFDWS
                     KTWVAEMFLSEAERKRRLHTQKAIEHELESQRGITPEVRRYNEIKAGIITSSIGLGSA
                     IFLFILMQGIIQGGQTPPHANEILSRVWIAGVVPFLVGIALIINGLFVSKKIVEVTRR
                     ESSRMADALGGDTARRSLNSADTSEISPASFSVTEETTKHLVNSRPKQ"
     gene            12704..14686
                     /locus_tag="VGB76_01070"
     CDS             12704..14686
                     /locus_tag="VGB76_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0097516.1"
                     /translation="MLLLLAAALVVLLVVLAWLQYRWLGQVSEAERERMQSTLSAGAA
                     RFSQDFDRELGRVYFGLKLDADAWGARDGEAYAARYEQWRATTPHAQLVRDVYVVDGG
                     TAGGQPQLTRFDAAARKFVPSDEAWPAEFDALRHRFEAAHEMSTPAHSELFAPQPNVD
                     HSIPSVIIPIVPDEPARPVEHGGEAGRENSVGDVEASGAGNERKLVLTNYSPLAAYAV
                     VRLDLEYIKREMWPALARRYFSSGDQLDYRISVVDANNPQKIIYQSAPGADTDAAATT
                     APAPGDARAGIFKIRFEEMDALVQPGRAVRRVSKEQETTVLPPARESSEKRVALRFYE
                     SRTSHVEMRRMRLQDIGEGNWQLVATHAAGSLDAAVGQARRRSLLVSFGILLLLAGSV
                     ALIFVSTHRAQRLARRQVEFVAGVTHELRTPLAVICSAGENLADGVIDDRRQVRRYGE
                     VIHSEGRRLSEMVEQVLEFAGAQSGRRELELAPVEVASLIDNALASCRAGAHPHEASA
                     CEIERDVPADLPPVLADFGALSRSLQNLLANALKYGGESRWVKLSARSVERVRGGGAE
                     VRIKVEDRGAGIAPVDLPHIFEPFYRGREVVAAQIHGNGLGLSLVKQIVEAHGGRVSV
                     RSEPGRGSAFTIHLPAAPGAPAAANGNGHHLRRSDI"
     gene            14683..15405
                     /locus_tag="VGB76_01075"
     CDS             14683..15405
                     /locus_tag="VGB76_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0097517.1"
                     /translation="MNQRILLVEDEPGLLMTLTDRLVSEGFAVETATDGEAGLQRALA
                     ESFDLIILDVGLPRKNGFDVCRDLRQRSIGVPVIMLTARGQVVDKVVGLKIGADDYVT
                     KPFEMMELLARIEALLRRVPPAHAAATGSAHGSAAGEAGDVYSFGAIHADFRRAEVTR
                     EGERIELSAREFKLLRYLIEHRGATISRDELLNEVWGYNAMPSTRTVDVHIAWLRQKL
                     EPNPHYPQFILTIHGLGYKFVG"
     gene            complement(15387..15713)
                     /locus_tag="VGB76_01080"
     CDS             complement(15387..15713)
                     /locus_tag="VGB76_01080"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY0097518.1"
                     /translation="MELSARATNGRETRGYNRNGRRSTFFRLFSTDLANFHDTRGRGY
                     ALISRSLQLAPVFSGTRLPVQTLFDYPEGGDSLDEFPDEFQALSRGQAIEVLEKLKEL
                     HHPTNL"
     gene            15766..16365
                     /locus_tag="VGB76_01085"
     CDS             15766..16365
                     /locus_tag="VGB76_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0097519.1"
                     /translation="MGGDEVVKENNVPATAPQLKPVPHSGGLEELDTLFQQHYDQVYR
                     TAYRITGSPADAEDVLQTIFMRLAGNTTKRDLAPSPASYLHRAAVNASLDLMRSRTRA
                     RSVPFEDVDSALLQSPILNPEAQHADSEMRALIRQAVARLGTRASEVFVLRYFEGYDN
                     GEIAEMLGMSQMVVAVTLHRARVRLRGEIGEYLEKHHEA"
     gene            16355..19135
                     /locus_tag="VGB76_01090"
     CDS             16355..19135
                     /locus_tag="VGB76_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0097520.1"
                     /translation="MKRNKKELETILDETMSDMRNEQLNTHTIDEAASRVWARIAADH
                     NPASVMTPAAEHINSCGDFQSLIPSYLRRELSPARTLLLEDHTQECIPCRKALKEART
                     GRAAATRATLKPARATQTNFTAWRWAVAASLAVAVAIGALFFSNRMFDTGGALAATVE
                     STSGALYKVTDAGTLAMAVGEEIGKGVHIRTAKDSGAIVRLADGSRVEMRERSEFSVS
                     ENGQGLTLHLDRGDVIVEAAKQGAKHLYVATPDSLVSVKGTIFAVGSGTKGSRVSVVE
                     GEVHVNHSGSDKVLKPGDQTTTGASLVPVSVSADVAWSRNAARYAKMVTELAALRKDI
                     AQQVPHPGVRYSTRFLDLAPEGTVLYAALPNLATTLGESHRIMQERIAQNPALSQWMK
                     EQETSSAHGPGLNQTMARVREFGEYLGNEIVVTAGTNAQGNPDAFLVLGELKNAQGFR
                     PFVEKQLGELKGVDKGAPSVRIIDDPNTVVAATPAATAAATTAPNTEKKTVAKKLAGN
                     NNNELYIWIRDDFFAASPKIESLRGLQATMSAPGTNRFVGTPFYARIADVYKEGAGLI
                     VAADLEKVIAQATSDAAKVGGQQQLDLYKQAGLFNLKHFVVEQKEVKAKTQSRAVLTF
                     NEPRRGIASWLAAPGPMGALKFISPDAHIATAFVVKEPTLLVDDLLGFMETASPDLRK
                     RLRELETQNGLDIKRDFAAPLGGEYAFAIDGPVLPTPSWKMIVEVYDQKHLQQTFENV
                     VGKLNAWAAQHGQKGLTLQQSGNIYTLRSVDFGLEVHYTFSEGYMIAAPTSALLTRAL
                     QYRESGVSLLTSPKFIAALPEDGNTNFSAVIYHNLAALLQPLAGAIGDRSKSLGEEQQ
                     KAIAAFAANSEPTLAYAYAQGDRITLAANTEGGPFGLSPGSLLGLPNAFAMQHIMMEA
                     MDEKKGSTPEKK"
     gene            19256..20023
                     /locus_tag="VGB76_01095"
     CDS             19256..20023
                     /locus_tag="VGB76_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0097521.1"
                     /translation="MKGGNLIAGILIVVSLWAVTVWFVFASGIADRHEPTTPLTVAAE
                     RGDAAQVRKLIAGGADLNERDDSGYTSLVWAARNGNTEVAEALIEARADLNARDCAAN
                     GWTPLIHAIHKNNNEMARLLIERGADVNAKAGKCTEKTIESGATALLHAAGEDNTEIV
                     RALLAKGADPYAEYDKANALSKAVAGAMDFSRPDDKQCPTETVKTLLESAPELTTKRS
                     VWDRKSVFIARHKGCTELVSLLEQRKSALAAKAAANN"
     gene            20467..21423
                     /locus_tag="VGB76_01100"
     CDS             20467..21423
                     /locus_tag="VGB76_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0097522.1"
                     /translation="MNPVIDLDGLGVTFGGRPILQDLRGTLSGRAIGLLGPNGAGKTT
                     LIHTLLGFHPATAGTARIFGQDISANAKQIRTLIGYMPERDSFIAKMSCVHFVRLMAE
                     LSGMPSDAALERAHESLFYVGLGEARYRTLDTYSLGMKQLAKLAQAIVHGPKLIILDE
                     PTNGLDPPTRLRMIRLIREIRDSGEARIMLSSHLLRDVEECCDEILVLKDGRIAANCN
                     LEEERKANRKFIELEMRGGDNRAFAEAIGQLGCEYAFTNERRLKLVLPEGIEIRDLYR
                     VAAGQAVQIRRLNYKRDSLEDIFLKAMEGGAVGTGGNGNGSL"
     gene            21410..22324
                     /locus_tag="VGB76_01105"
     CDS             21410..22324
                     /locus_tag="VGB76_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0097523.1"
                     /translation="MAVYEHGYKPYTGGLTPEWSRFLIIPRHAYRDVFRSKFFIAFFA
                     LCFIYPLVAAILIYLHHNVNAIALMQINVRELVPINASFFHVFMTTQCSLGFFLTVLI
                     GPPLVSRDLANNALPLYLSRPFTRKEYVVGKMSVILVLVSLITWVPGLLLFILQAYLE
                     GAGWFGNNLWMAGAIFLSAFVWIAVLSFLSLAVSAWLKWRVVASGALLAIFFIPSAFG
                     EIVNALFMTRIGHLISIGAIMNSIWLGLFGLFQRQTGRMETTQGGRRIEMVFYEPPLW
                     SSWLALGLICAFCVLLLMRKVRAYEVVK"
     gene            22321..23319
                     /locus_tag="VGB76_01110"
     CDS             22321..23319
                     /locus_tag="VGB76_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0097524.1"
                     /translation="MTTAPATVATNANVTAHAVERMIVFEDVSKFYGEILGVNRVNLS
                     IAPGITSLVGPNGSGKTTLMNLMTGLLKPTRGHISVLGTSPHNPQELFRKVGYCAQFD
                     SFPRGATGREFIEFYLRTHGGTRRAVENLTYQALERVSMLEAADRKVAGYSKGMRQRI
                     RLAQSIAHNPAVLILDEPLNGLDPMARAEIIRLFRQLAAEGLYLIISSHILHEVDMMS
                     DSVVLLNNGYVVAEGNVHGVRDEIEEHPMQILIRCDRPQVLAARVFEQDDGAVVEARL
                     HDDRQGLFVKTRRPDDFYLLLNRVVLDGDLKIESVAPADDDLNAVYQYLISSDGGR"
     gene            23319..24194
                     /locus_tag="VGB76_01115"
     CDS             23319..24194
                     /locus_tag="VGB76_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097525.1"
                     /translation="MSSTIDSATPAQIKSAREMPWQLWAQQTLAILRLELQKNFLSRR
                     ALLIYLLAALPILLLFVLAVLPPSEDDTFNNFAELGVLFSAIYNGLILRTVVFFGCAW
                     IFMNLFRGEVVDRSLHYYFLSPVKREVLVVGKYLSGLVTAIVLFGLTTLVSLFMLYFP
                     RFSAESSRYFFGGPGGKQALIYLGITTLACIGYGAIFLIVGLFFRNPIIPAILIYGWE
                     WINFLLPPLLKKVGVIHYLHSLMPVPMNEGPFAVMAEPTPAWISVPSLIIFTIIVLIL
                     AGLHIRRMEISYIGD"
     gene            complement(24370..25443)
                     /gene="rtcA"
                     /locus_tag="VGB76_01120"
     CDS             complement(24370..25443)
                     /gene="rtcA"
                     /locus_tag="VGB76_01120"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF003246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEY0097526.1"
                     /translation="MKRRRRSGYNRLIMLTIDGSQGEGGGQIIRTSLALSLITGKPFR
                     IYNVRARREKPGLQRQHLTAVLAAAAIGGAQVEGASLGSSAFTFTPGTVKPGDYSFPI
                     GTAGSTSLVLQTILPPLMIADAPSTLAFEGGTHNIHAPPYEFIARAFLPLINRMGVRV
                     SAELERYGFYPPGGGRINVRIEPVARGALAPLELLTRREIRARRARALVVKLPPTVGE
                     RELTVVREQLGWNEEEGDELRVETSTNAHSPGNVLTLEIESEHLTEVFTGIGERGVRA
                     ETIAERASDEAAAYLETGAPVGEHLADQLLIPLALAGGGSYTTGQPTLHTTTNIEVIR
                     MFLDVEIKAGQAEADVWKIEVSG"
     gene            25495..26919
                     /locus_tag="VGB76_01125"
     CDS             25495..26919
                     /locus_tag="VGB76_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY0097527.1"
                     /translation="MAKSIVLRFDAGTLLLDGLERTPPVAGDVPESFRWDERVRRWRA
                     PASAYRHVVRELIRKKVAFEDQARAYHKFDFQTKFTVEPRPYQQEAIAAWRKAERCGV
                     VILPTGAGKSLVAQMAIEQTQRSTLVVVPTIDLMNQWYDLLLSCFQAEIGLIGGGFFE
                     VGALTVTTYASAFRFMERLGNEFGLVIFDECHHLPGDVFRYAAEMSLAPFRLGLTATP
                     ERADGADALLEELIGPIVYRREARELAGEYLSDYSIVRLHVELSAAERAGYEASRAVF
                     KSFLQEKNIRLSSLHGWQLFIAASARSEVGRRAMMAYRESKRIALGTEAKLRVLAELL
                     KRHRRDRVLIFTAENEMVYRISEQFLIPAITHETNIKERREWLAAFNAGDVLALATSK
                     VLNEGVNIPAASVAIVLSGSGSTREHIQRLGRILRKQPEKEATLYEVVTLDTTEEGIS
                     RRRGGEQFRAGTGRSEGGRLPGGL"
     gene            26923..28152
                     /locus_tag="VGB76_01130"
     CDS             26923..28152
                     /locus_tag="VGB76_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEY0097528.1"
                     /translation="MLTADLALSWQRGAQIKPRYIDAEDADWLDAASALIELFREHRG
                     CTRAELEAALEEYVGAGTDYKILRGLIKLLTDRAEFAAGVEVVPADLRRAVFLKARGA
                     HPVTDESVRAQVLTEAGEELGCATELVRANLYADLPKNQRLVEFEETDARALLELYNV
                     AQAQALLYRCVRMRLSIEPQGVENYRELFGAIKAYRLIHTIKGSPARGYEIELDGPVS
                     MFHRSQKYGIQMAVFLPALLLCKGWRMRAEIESRHTGTSFFEMTSEAHGLRSHYLSVT
                     PYENPVREKLLQSWARFESEWTLETSGEIIDLGASAFIPDFVLRHAGGRVVYLEILGF
                     WTPPHLKERLQEFAGSGFRDFLLAAWEELRGSREPLVAAPPHTLIFKRSLDPATVELA
                     VNRLISEAGQTQGGELA"
     gene            28149..29171
                     /locus_tag="VGB76_01135"
     CDS             28149..29171
                     /locus_tag="VGB76_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097529.1"
                     /translation="MKTFNLCFLGFGNVGRALLRLLGEKSVALREEYGIEWRATGVAT
                     RRMGWIASADGLPSESLLAGEATVRREGESQPANVREWLAAARCDVLFETTSLEPLTG
                     QPAIEHARAGLDAGAHVVTANKGTIVHGYRELDALARERDRRFLFEATVADMPVFSLF
                     RECLPAARLLGFRGIFNSTTNIILEEIEAGRTFAEGVRRAQLLGVTETDPAYDVDGWD
                     SAVKTCALVNVLMRAPLRLDEIAREGIRELDAASVRAAHAAGKPYKLVSRAELETGGR
                     VSARVRAEQVATDDPLAVAGGTSLSINFELDILPGLTLVAHQPDLQSTAYGLLADFVN
                     AVRAET"
     gene            complement(29318..30253)
                     /locus_tag="VGB76_01140"
     CDS             complement(29318..30253)
                     /locus_tag="VGB76_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097530.1"
                     /translation="MSSAIFDQPAHEPTPQSLAYLPASEASSGEEINLETFLLPTVEV
                     PLSAPLAMLVESLTPGSFRETMRRLFVDLARLLDYLRLIEKKVQQDQLLASTFSVFNN
                     VHKESLALIENIEAGILSMDGADEELLSTLDGISYAIGHEIGRVYGFELVGISDPHSP
                     PIRGKLERAHGMLRNCFQQSTISLTQVFNPALDGEELFNDFHARREEALILYRDLWNL
                     VQLARRADQECDQFSVALLLAHLKRFRDGSMCYLMYKDWEEYERFVKKVAAARRVLEI
                     GPALEGLACYLETLLGQVRLRSVLANLAFIVPDSD"
     gene            30745..31443
                     /locus_tag="VGB76_01145"
     CDS             30745..31443
                     /locus_tag="VGB76_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0097531.1"
                     /translation="MINVRRAAERGHFNFGWLDTRHTFSFGEYYDPRNMGFRTLRVIN
                     EDHVRAGHGFPTHPHRDMEIITYVLEGALEHKDSMGNGTVILPGEVQRMSAGTGIRHS
                     EKNHSTEQDVHLLQIWIIPEEQGLTPSYEQKMFEADEKRERLRLIASNDGRDGSVTLH
                     QDASLYAALLEPGQEVTYQLSPGRHAWAQVARGALEINGQLLQQGDGAAISNESQLKI
                     AGRDSSEVLLFDLG"
     gene            complement(31587..>32060)
                     /locus_tag="VGB76_01150"
     CDS             complement(31587..>32060)
                     /locus_tag="VGB76_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0097532.1"
                     /translation="AEAEDLAQEAFIQLFRKIGSFRGESAFTTWLHRLTVNQVLMHFR
                     KRGVRLEQTTDDGETPVQIVAGTQNPNSMPVVDRIALDRAIGQLPPGYRTVFVLHDVE
                     GHEHEEIARMLGCSVGTSKSQLHKARMKLRTLLRQQKPSRLSENSFVTRAKPDIA"
BASE COUNT         6224 a         9553 c        10220 g         6063 t
ORIGIN      
        1 cgcccttcac gatcaactat ctcggcaaag accccgccga cgatccggag atttatcaaa
       61 agacctcgcc catgtcttac atcaagagcg cgcgcacgcc gacgctcatc cagcacggcg
      121 aactcgaccg ccgcgtgccc atcgccaacg cctacgaact gcggcaaggc ctggaggaca
      181 ggggcgtgcg cgcagagatg atcgtctata aaggcttcgg ccacggcatc acgaagccgc
      241 gcgcgatgcg cgccgtgatg catcacaatc tggcctggtt caatcattat ctctggaacg
      301 agccgctgcc cgatttagcc aacttgccgc tgccgaagaa ggaagagaag gacggcgaga
      361 agaaggacga agagaaaaag acggcggcag cagccgcagc gaccaactaa ccgtccgcgc
      421 gcgccgactt gaaaaccacc gtccgaagtc gccgccgcgc gcgttcaaac tgtcagtcgg
      481 gcgcgtccga catttccgtc gagttcgaca tcaacgcaaa gtttacggga gagacacaca
      541 catgagacgc atcgcacctt tcctcctctg cctctgcttt atcactaccg gccaacactt
      601 gacggcgagc gcgcagaact ggccgcagtt tcgcggcgca caggcttcgg gcgtcgccca
      661 aagcacgcag aagacgcccg tgagttggaa cggcgagaag tccgtgggcg tgcgctggaa
      721 gacgccgatt cccggcatcg ggcaatcgag tccggtggtg tggggcaacc gcgtctactt
      781 gacgaccgcc gtgagcgcgg aagcgaagcc cgccgcgcgc tatggactct acggcgatgt
      841 cgagccggtg aaggatgaac cgaagcacac ctggcacgtg atggcgttag acaaaacgac
      901 gggcaaggtc gtctgggatc gcaccacgct cgaaggcttg ccgaagacca aacgccaccc
      961 gaagggcacg cacgcttctt cgacgcccgc gaccgacggc aaacatgtcg tcgcgctctt
     1021 cggctcgcaa ggggtcctgg cctgctacga cacgaacgga aagttgctct ggaagcagga
     1081 gatgggagtg ctcgacgcgg gctggttcta cgatcccgat tatcaatggg gacacgcgag
     1141 ttcgcccgtc atctacaaga acctcgtcat cgtgcaggcc gacatccaga aaaactcttt
     1201 cctcgcggcc tatgatctca agaccggcaa acaggtctgg aagaccgtcc gcgacgagat
     1261 tccttcgtgg ggcacgccca ccgtctatga aggcaagacg cgcccggaac tgatcgccaa
     1321 cgcgacgaag ttcatacgcg gctacgatcc tctgaccggc aaagaactct ggcggctcgg
     1381 cggcaactcc gagatcacca cgcccacgcc gatcatcgcg cacgatctca tctacgtcac
     1441 cgccggttat cgcccgatcc agccgatcta cgcgatccgg ctcggcgcaa acggcgacat
     1501 cactcttaaa gagaagcagg agtcgaacga gtttgtcgcg tggagcaagc agcgcggcgg
     1561 cccctacatg ccgacgccca tcgtctacgg cgactacttc tacacctgct cgaacaacgg
     1621 cgtcgtcacg gcctacaacg cgaagacggg cgagcgcgtt tatcaggaac gcatcgcggg
     1681 caagggcggc gcgttcaccg cctcgcccgt cgcggcggac gacaaactct atttttcgag
     1741 cgaagacggc gaagtcttcg tcgtcaaggc gggacccaca cacgaactgc tggcgacgaa
     1801 cccgatgggc gagccgctca tggcgacgcc cgccatctcc gacggcatgg tcatcgtgcg
     1861 cgggcaacat cacctcttcg gcatcggcga gggcgcaccc gctccgacga tgaagtcgaa
     1921 ggcgaaggcc gcaaagtaaa ttactctggc gcacgcacaa cttcagtggc gcgcgacaag
     1981 accagttcct gacttccttt tgcgcctccc gcgtgcagat acagtcgcac gcgggagacg
     2041 cgaaggaaga gtcaaagggt tgagtgatta gcgtagtttt aggcagtctc cacgcatgag
     2101 cggcgcgcgc tcgaaatcga aaatctgcaa atggaacaaa agccggtgtc gccgcgtcta
     2161 agcgacattg gttttaccct ctattggaag aaggaaacca acacatgaaa cgtttttttg
     2221 ccgggttgtt attgtcaacg ttcgtctgcc tcgtcgccgt ttctccgctg agttcggttg
     2281 cggttaaagc caaaggtggg aactggccgc agtggcgtgg tcccgaaggg cagggcgtgt
     2341 cgatggagac gggcttgccc acagaatgga gtgcgaccaa aaatatcaaa tggaagacgg
     2401 cgattgccgg gcgcggccac tcttcgccca tcgtctgggg caagaaaatt ttcctgacca
     2461 ccgcgctcga cggcgcgccg atcccgggcg cgaagcccgg agtcacgcac aagatggccg
     2521 acgggtcgga cttcgtacac cccgacgccg tgggcgcaga cctcaaacac acgttcaagg
     2581 tgatctgcgt cgaccgtgag acgggcaaga ttctgtggga gcgcgtggcc tacgaaggcg
     2641 tggtctatga cagccgccac aagaaggcga gcttcgcttc ttcgacgccc gcgaccgacg
     2701 gcaagtacgt cttcgccttc ttcggcgcgg aaggtctgta cgcctacgac atgggcggca
     2761 aacttctctg gaagcagaac ctcggcaaac tcggcacggc gagcgtgggt tacggggtgt
     2821 cgcccatcct ctacggaaac ctcgtcatca tgcaatgcga cgacagcggc atgaactctt
     2881 tcatcgcggc gtttgacaag aagaccggga aagagatgtg gcgcgcggcg cgcaaagttg
     2941 acttgacgtg gagcacgccc gtcctcgtca acacgggcaa gcgcaccgaa ctcatcgcga
     3001 gcgcggcgga agcgatcatc gcctacgatc cgcagacggg caaagaactc tggcggcaca
     3061 agggtctgga gagcaacgcc gtgaacacgc ccgtcgtctc gcaagacatg gtggtcgtca
     3121 cgtccggcta tccggcgaag atcgctctgg ctctcaaggc gggcggcagc ggcgacatca
     3181 cgggcacgcc gcaactcatc tggaagtaca acaaggggac ggcctacgtg ccttcgccga
     3241 ttctctacgg cgagtatctc tatttgatga cgggcaacgg ctcgatcacc tgtctggaag
     3301 cgaagaccgg aaaggtcgtc tacgaagcgg ggcgcgtgcc gaagcctact tcattcatgg
     3361 cctcgcccgt cgcctacgag ggcaagattc tgttgacgag cgaagagggc gacacttacg
     3421 tcttgaaggc gggacccaag cacgaagtca ttcgcacgaa cacgctcggc gaacccgtct
     3481 atgcttcccc ggccatcgcc gacgagaaac ttttcatacg cggcgagaag aatttgtact
     3541 gcatcactaa cgcgcgtggt tagataaccg gagttaagaa acgatgaagg aggaacgatg
     3601 cccgatgaat gaaaagcagg agtctttcat tcatccttca tcgtccctcc ttcatcgttt
     3661 gccttttgcg ttgtggtgtc tttgatcgcg ctcgactaga attgcgccac gccatataag
     3721 gactcggatc aattgccggg ttcgttcgtt tcgttttccg gcaagggagt taagcaatgt
     3781 tcgaatcaca agacacgact ccgtggtatc gctcgcgctc gacgatcatc ggcgtatcag
     3841 tctgctgcgc cgcgctcgtc ctcacgctgt tctatcattt ctacggctat cgctatttca
     3901 ccaaccgcga cgcgcactac gccgaactcg aacgccaccg cgcgcagcag gccgcgcttg
     3961 ccggaaacga accgccgccc gccgctcccg ccggacaacc aaccgcgccg gagcagaacg
     4021 caccgaccac cggacaaccc acgagcgcgc cgggggcgac ggttgcagag gcgagcggaa
     4081 acagcgcgac ggcgaacggg caggacgcga atcatccggt cgcccctaac gcttcgtccg
     4141 cgcgcaacta ctggacgaac tttcggggtc tgaatcgaga cgggcgttac gaagagggtg
     4201 cggtgaaggt ggactggcct gcgggcggtt tgaagccgat gtggaagcag cctgtgggcg
     4261 gcggcttctc gtcgttcgtc gtggccgacg gcacggctta cacgattgag cagcggcgca
     4321 aacaggaagt ggccgtcgct tacaacgtcg agacggggcg cgaactctgg gcgcacggtt
     4381 ggaacgcgga gttcagcccc gatggcaccg gcgacgggcc gcgctccacg ccgacgtggg
     4441 acggcgggcg tctctatgcg ctcggcgcgg caggcgaact gcgcgcgctc gatgcgaaga
     4501 cggggagact cttctggcag aagaacattc tcgcggacaa cggcgcgtcg aacattcaat
     4561 ggggcatggc cgcctcgccg ctcatcgttg acgacaaggt ggtggtgctg gcgggcggca
     4621 cttcgggcaa aggcgtcgtg gcttacaaca agttgacggg cgcgcgcgtc tggtcgtctc
     4681 tgaacgaccg cgcctcttac acgtcgccga tgctcgtcac gctggcgggc aagcgtcaga
     4741 tcatggtcgt tacggcgagc cgcatcgccg gtcttgaccc ggcagacggt gcagtgctct
     4801 ggacgcaccc gtgggatgtc tccatgggca tcaacgtttc gcagccgctc atcacgggcg
     4861 cgaatcgttt cttcatctcg gcgggctacg gcaaaggcgc gacgctcgtt gagcttcagg
     4921 ggagcggcaa gagttttacg acgaagagca tctgggagaa catcaacctc aagacgaaat
     4981 tcaacagccc cgttgtgcac aacggccacg cctacgggtt ggacgaaggc atcctgacct
     5041 gtctcgatct ggcgacgggc gagcgcaagt ggaagggcgg acgctacggc tacgggcaag
     5101 tgctgcttgc cagcaatcat ctgatcgtga cgacggacac gggcgatctc gtcctcgtca
     5161 aagcagaccc ggcagcttac gcggaagtcg ccaaattcaa cgcgctctcc ggcaagacct
     5221 ggaacgtgcc cgcgctcgcg ggcggacgcc tcttaatgcg caacggcaac gagatggccg
     5281 cgtacaatct aacggattaa cgcaaggcgt attcgcctgc ggcaaactaa aaaagcagcg
     5341 cagcccgaag gaactgtaaa gtcgccttcg ggctgcgctg cttttacgtc ggttagtgaa
     5401 caggggcgcg cgggttcggc tcagtgcagg ccgccgcctt tgccgcccgc gctctctttg
     5461 ctctccttcg gcgtccgcgc gcttgcagag gcttcgtcgt cttcgcctgc gccgcgcgct
     5521 tcttcgttcg gcgtgctgct cgcgggcgag ccgcccttgc ccgccgtcgg cgtcttctcg
     5581 tactcgcgtt ccttatcgtc cgccatcgtc tttgccctcc ctgcacgtag agattgaacc
     5641 gcacacattt tatccacgcg acgcccgcgc ggccaacagg tgcgacgctt agaacaacag
     5701 tttcgcgccg aactgcaagt ggcgcgggtc gcgcgcggag agaatctggc cgaacgtggg
     5761 cgacccgagg aagttgtcgg gcaggttgaa gttcggatga ttgaaaaggt tgaaggcttc
     5821 gagccgcagt tgcaggttca cgcgctcgcc gagccgcgtg ttcttcaaga gcgatgcgtt
     5881 gacgttctgg tagccggggc cgtcgagtgt gttgcgcccg gagttaccga aggtgccgaa
     5941 ggccgggaag acgaacgcct gcgtattgaa ccaacgttcg ggcgtgcgat tcgccacgtt
     6001 gggatcgccc gcgacgttcg ggcggtcgtt cgcgccgaag ccgagcgtgg agcgtcccgt
     6061 gttgctgttg tcgatctccg agagcagcgc gacggtgaac gggcgacccg tctggagcgt
     6121 cacgatgccg ttgaactgaa agccggagag aagagtggtt aagaagcctt cgtcgctcag
     6181 aaacttgcgg ccatgaccga aaggcaactc gtaggagaaa ctcgtggaga ggcgatggcg
     6241 cacgtcgaag ttcgagcgac cgtgttcggc gcggaggttg aggctgtcct gcggaaagtt
     6301 gggatcgccc gcgctcgtaa agaagttcga ggcgtcgtcg agagatttcg cgtaggtgta
     6361 ggacgagagc agcgcgagtc cgttggtgag acgttgttgt agacgcacct gtaagctgtg
     6421 atagttggag ttgccgcgcg actcgatgag attgatatcg tcgaactgcg gcacggggcg
     6481 caggacgaaa ggcagagtgg aggggcgcgg ctggttgatg tcgcgtgcgg tcagaagttt
     6541 tgtgcccttc gatccggcgt agccgatttc gaggacgcgg ttctgtccga gttgctgttg
     6601 caggttcagg ttccagtgct gcatgtaggc cgtgcgcagg tcgcgctgga atgagagcgc
     6661 ggtgtcgggg agcggcacgg ggaagttgct ggggaacggg ttgttgacgg tgagcggcag
     6721 accttcgagc gagaagtaga gattgaagtc gaagtagggg gcgttgaaat agagtccttc
     6781 gccgggggcg agcgcgggct gatcgtaata gacgccgtag ccgccgcgca cgacggtggt
     6841 gcccgattcg ttcagcgtgt aggcgaggcc gaggcgcggc gcgaagttgt tacggtcggg
     6901 cgcataaccg ccgcgcggga tgccgttcgt gccgacgcgt gagagcgtgc gcgtcgcggg
     6961 gtcgtagagg ttcgcgcggt cttccacatc gacgggcggc gagttgtatt cgtagcgcaa
     7021 gcccgccgag atggtcaggc gcggcgtgat gcggaaactg tcgttggcgt agacggcgta
     7081 gctttcgccg cgcaggtgtt gatgattgtc gagccgcgcg ccgcccgtga gcgcggggaa
     7141 gccgagcagg aggtctgcaa gcgcgttgcc tgtcagaggg aaacgctcgg agaaggtcag
     7201 gaagccgcgc gattgcacgt cgcggaaagc gttctgctgc atcgcgcgaa actcgaagcc
     7261 gaacttggcg agatgcgccc cgcgcacgta agagaggttg tcgagaaact ggaatacgtt
     7321 ggtcgcgctc tgctgcgggt tgttgaactc gtcgccgagc ggcgagtagc ccgtgatggt
     7381 gatgaagctg agtccgaagt cgcgcgggtt gttggagagt tccggcaggc cgacctgacg
     7441 attgagactc gcgcccgccc cttccgacgt gaccgccgac gccacgcgac tgaaagcgaa
     7501 gcgggcttcg ttgacgacgc tcggcgagaa cacgtgcgtc tcgctgagca tcaaattttg
     7561 gctgcgacgg gggacgaagt tgccgaagcc ggggacgagc gaaaaggtcg tacccgtgaa
     7621 ggggtcgaag aggttgcggt cgccgaaact gtaacgcgcg gtgaacgtcg aggtatcgct
     7681 gaacctgtga tcgatgcgtg cgtcgaaatt gtcgttgcgg tcgcgcaggg tgggcgacga
     7741 gacgaagttg gcgaagggca cgttgcgatt gggcagcggg tacaagttgg cgatggcgcg
     7801 cccgatgggg ttctgaaact cgacggggat aacgccgttt gtgaaaggct gctgcacgaa
     7861 ggggttgaag gggcgggcga agagcgattg cgagaagtcg ccgttgcgtt cggccagcgt
     7921 cgggacgttg gtgatgcgtg tgatgccttc gcgcgtgcgc gtgccttcgt agtcgccgaa
     7981 gaagaaggtg cggtctttga caatcgcgcc gccgagcgag aagccgaatt ggttgcggat
     8041 atatttcggg tcgggctggt ctgcgggcgc gaagaagttg cgcgcgtcga aggcggcgtt
     8101 gcggtgaaac tcgtagagcg tgccgtgcag cacgttcgag cctgacttcg aaatgacgtt
     8161 gacctgtgcc ccggcgttgc gcccgaagga agcgtcgtag gtgctcgttg aaatctcgaa
     8221 ctcctgaatg ccgtccacgg gcgggcggac gccgaagctg ttgagtttcg ggtcgaggtt
     8281 atagacgccg tccagaagaa agttgttgga gtcttcgcgt gcgccgttgg cgctgaaggc
     8341 gaagtcgccg cgcacggaac cggctgagcc gggagcggag ggcgcggtgc cgggggcgag
     8401 cagcgcgagt tcgaggaagt tgcgcccgtc gagcggcaac tccgtgatct gtcgttccgc
     8461 gatgaccgtg ccgagcgcgg cggaatcttt tctgagcggc gcgggttcgc tgatgatgcc
     8521 gtcgacggtg atcgtcacgt cggtcacgga gagcgcggcg tccagtctca attcctgatt
     8581 gacttgaagc gcgacctctt gcggcagacg ttggaaaccc gccgcttcga cggccacgag
     8641 atagacgccc ggcgcgagcg ataaaatggc gtactcgccc gacgcgttgc tcgtcacagt
     8701 cctgatctca ttcgtggctc tattcgtgag cgtcacggtc gcgccgggaa cgacggctct
     8761 gttcgggtcg ctgactgtgc cgcgaatcga ggcgcggtgt gtttgcgcga aagtcgcggg
     8821 ggcaatgagt aagacgagca ggaaagagag aaaagtgatt ttagacttca cgacgactcc
     8881 tcgcggtttt tgacggttga caggatcgag aactgtagat ggatgaaaac gcaccttacc
     8941 ttaacgaacc ggagcggata aaagttttcg ggttggacgc aggcggcaag gtttttcttt
     9001 catccgaaaa ttcggaagag acagccccgc gcgcgtcgcc tgccggaatc cgcaaggtcg
     9061 aagctcacgc gcgggactgt ctctttcgag ttaaacgaac ttcacaagtc gaagaattgc
     9121 acaggtcgaa cgaatctcgt cgtctagctc ttcttcgtga agatgctgca cgcctggtat
     9181 gcgcccgaag ggtcgatggc cgagcgtccc tcttcaatgt ggcggatgat accttccttg
     9241 tccacgacga acgtggcgcg ggcggagtag ccgcttttct cgtcgagcaa gccgtagtct
     9301 ttaacggcct gtcgtttcca atcgctgagc agcgggaagg tcactttgat gtcttcggca
     9361 aagcgtttat tggccgcgaa gctgtccatg ctgatgccta agatttgtgt ttcggtggcg
     9421 tcgaatttgg cgatatcaga ctgatacgcc tgtagttcct tcgttcaacc gccggtaaag
     9481 gccagcacgt agaaggccag cacgacgcct tttttgccct tgaagtcgga caaacttacc
     9541 ttctggcctt gcgtgtcggt gagcgtgaag ttgggcgcgg ggtcgccgac tttcaaatgc
     9601 gtcttgggcg cggcgggctg cgccccggcg ttggcctgcg gctgcgcggg cgcggcgggc
     9661 tgcgcctgcg gcttctgttg cgcgcaggcc gcgattgaca gcgcgagcag cagcgtaaaa
     9721 cttaaaaatt ttcggttcat caaacttagc tcctcaaatg tggaaaactc gtccggggaa
     9781 acttctctcg aagccatatt agacatgatt gacgataggt tggcaacaac ctgacccgcg
     9841 acgcgcgacg cccctgttgg ccgcccgtcg agactcacgc tgcgcaagcg gttcttcaat
     9901 acaagaacag gacgggcgcg ggttgtcgta tctcaacccg cgcccgtccg cctcgccgcg
     9961 cgcgacgcac gacgaaaaga gatgcccgcg tcagtctttc gtcacgaact gctcgatctc
    10021 tctgacgagc ggcatgacgg cgggcgggtc gtagtttgac agcacgatga gcgtgtcgcc
    10081 tgtgtctgtg gcagcggcct tgtcgagtag gttctccaac tcctgcaaac gctcccgctc
    10141 gtctcacccg tcgcgcccgc ctgtgtcgct ggcggcgggt tgaacgccgc cagcgtgaag
    10201 gcgaggataa aacatatcga cgcgtgcata agtgtgatct ctcctttcag gcgggctagc
    10261 tccgggagct ttccttcttc aactcgtcca actctttctg caacgtctcg atgaggctct
    10321 tgagttgagc ggaggctttc gcgtcctgtc tctgcgcgga gagtttcttg agcgcggcga
    10381 gttgcgcgac cttgaaggcg tgcctcgcag gcgttttgat gtcaggctcg acgcccgtgc
    10441 cttcccagtt ggtcttcgtg atggggctga tggagcgcgc gaagggaacc attacgaaga
    10501 agtgatcgct gagacgcttt cccatgacgg ggtgcgcgcc gcctccggtc gtctcgccga
    10561 cgatggttgc gcgcttgaga tttttgagcg tgtaactgat gtcttcggcg gcggagaagg
    10621 tgaaactgct cgtcaggata tagacatcct tccgaccgta gcgtctgccg ggcacttccg
    10681 cgagcgacca gaacgagatg gtttcgtcca tcacgcggtc gtaggcgtcg ctcaggtgga
    10741 tggacttgtc gaaaaagtag ctcatcagca gcgtgatcgc ggcgctcgct ccgccgcgac
    10801 aacggcgcag gtctatgatg agcgcgtctg tgtcggccag acgattcatc gcgtcggaaa
    10861 tcttctggcg cgcctcgccc gacgcgtcga agtagctgaa ctccaacagc cccagattgc
    10921 cttcgagcgt ctcgactttt tccagcccca tgttgcccgt caggtcgttc gggttgcgcc
    10981 gcacgacgcg ctcgccggac gccggattac cgcccccgcc gccgtcgtcg cccggcctgc
    11041 gacggacgat gcgcgcgccc ggcaacgcct cgccttcggc caacgcttcg gcgcgatagt
    11101 tcacgcgcag gtgcttgtcg cggctgacgg cctgcaagtg ctgtgtgagc atctgcgcca
    11161 gagccttcgc gctcgtaacc tcttcgtact ccttcttcgc cacgcgctcg cgtatggcct
    11221 gctccatacg cttcgcggtt tcgggataga cgtaagcctc gtgcaaattt ttcagcgcgc
    11281 cttcgatgac ctgcgtgcgc acgcccgcgt cgatggtcat gtcgggttgt tccggttgac
    11341 gcggcggctg cgtcgtcgcc gtttgctgcg ccgcgttgaa actcgcgagc gcgacgatga
    11401 agcagagcgc ggggagacag agactggccg ccctcgtgcg cggcgcgtcg aagagagcgc
    11461 gcgaggaact gttttgaaga cggggatgcg tgcggcgcgt cgtcggacgc aggatgcgtg
    11521 agagtgtcgg atgcattttg tttcttcctt tcaaactttc cgaatgatgg ctcgtctgca
    11581 agcacacggc ggatattagg cgagacgggc gcgcgagtgg ttaagccgag gaaaagtgtt
    11641 tgttaaggat gtaaaatctt tctgcccgta gttggcggcg agcgtccgcc cggtatgttc
    11701 gcaaaacttg aacaaacggc ctagaatcac gtattggaat tggagaaacg gatgggggag
    11761 gatgaggcgt ttatgttctg tccacagtgc ggcgcgaatc agagcgaaga gttgaagttc
    11821 tgcaacctgt gcggcgcaaa cttgcaggcc gtgcggcagg tggttgcgct gcgcgaaact
    11881 tcggaagata aattcgactg gagcaaaaca tgggtcgcgg agatgttcct ctccgaagcg
    11941 gaacgcaagc gtcgcctgca cacgcaaaaa gcaatcgaac acgaactgga gagccagcgc
    12001 ggcatcacgc ccgaagtgag acgctacaac gagatcaagg cgggcatcat cacgtccagc
    12061 atcggcctcg ggtcggccat cttcctcttc atcctgatgc agggaattat ccagggcggc
    12121 cagactccgc cgcacgctaa cgagatcctg agccgtgtct ggatcgcggg agtcgttccc
    12181 tttctcgtcg gcatcgcact catcatcaac ggcctgttcg tgagcaaaaa gatcgtcgag
    12241 gtcacgcggc gggaaagttc gcggatggcg gacgcgctcg gaggcgacac agcgcgtcgc
    12301 tcgctcaatt ccgcagacac gtcggaaatc tcgcccgcca gtttcagcgt caccgaagag
    12361 acgacgaagc atctggtgaa ctctcgtccg aagcagtaac gccgcgcgct aaagtggcgc
    12421 gcacgcgcga acgacaagac tgccccccga aattcacaca aacaaactcc gccgcgcgta
    12481 gcaaaactac cttccggctc tactttgctt tccggcgtga gtttgcttcc acgcaaaagc
    12541 gcaggggaaa gcgtggagcg ccgggtagcg tctcgccttg aagccgggcg cgagggcggc
    12601 ggatgttaag aatgtaaaat ctactgcgtg cccggtttca ggccgcgcgg gctgtgctac
    12661 attgccgggc gtgagaggca atacgggaaa gcgtcaacct ccgatgctgt tgctgttggc
    12721 agccgcgctc gtcgtgctgc tcgtggtgct cgcgtggttg cagtatcgct ggctcggtca
    12781 ggtgagcgag gccgaacgcg agcgcatgca gagcactctc agcgcgggcg cggcgcggtt
    12841 cagccaggac ttcgaccgcg aacttggccg cgtctatttc ggtctgaaac tggacgcgga
    12901 cgcgtgggga gcgcgcgacg gcgaggctta cgccgcgcgc tacgagcaat ggcgcgcgac
    12961 gacgcctcac gcgcaactcg tgcgcgacgt gtacgtcgtg gatggaggca cggcgggcgg
    13021 ccagccgcaa ctcacgcgct tcgatgccgc cgcacgaaag tttgtgccgt ccgacgaggc
    13081 ttggcctgcc gaattcgacg cgctgcgcca tcgcttcgag gcagcccacg agatgtccac
    13141 gcccgcacac tccgaactct tcgcgccgca gccgaacgta gaccactcga tcccgtccgt
    13201 catcattccc atcgttccgg acgaacccgc acgccccgtc gaacacggcg gagaggctgg
    13261 ccgggagaat agcgtcggcg acgttgaagc atccggcgcg ggcaacgaac gcaaactcgt
    13321 gctgacgaac tactcgccgc tcgccgctta tgccgtcgtc aggctcgatc tcgaatacat
    13381 caagcgcgag atgtggcctg cgctcgcgcg ccgctatttt tcgagcggcg atcagctcga
    13441 ttaccgcatc agcgtcgttg acgcaaacaa cccgcaaaag atcatttatc aatccgcgcc
    13501 cggtgcagac acagacgccg ccgctactac tgcgcccgcc cccggcgacg cgcgcgcagg
    13561 catcttcaaa attcgttttg aggagatgga cgcgctcgtc cagccgggtc gtgcggtgcg
    13621 ccgtgtgagt aaagaacagg agacgactgt gctgccaccg gcgcgggagt cgagcgaaaa
    13681 acgggtcgcg ctgcgcttct acgagagccg cacgagtcat gtggagatgc gccggatgcg
    13741 tcttcaggat atcggcgagg gcaactggca actcgtcgcg acgcacgccg cagggtcgct
    13801 cgacgcagcg gtcgggcagg cgcggcgacg aagtctgctc gtcagtttcg ggatcctgct
    13861 gctgctcgcg ggaagcgttg ccttgatctt cgtctccacg catcgcgcgc agcgtctcgc
    13921 gcggcgacag gtggagttcg tcgcgggcgt cacgcacgaa ctgcgcacgc cgctcgcggt
    13981 gatctgttcg gcgggcgaaa atctggccga cggcgtgatt gatgatcgca ggcaggtgcg
    14041 acgttacggt gaggtgattc atagcgaggg gcggcgtctg agcgagatgg tcgagcaggt
    14101 gttggagttc gcgggcgcgc aatcggggcg acgcgaactg gaactcgcgc ccgtcgaagt
    14161 cgcgtctttg atcgacaacg cgctcgcctc gtgtcgcgca ggggcgcacc cgcatgaggc
    14221 ttccgcgtgc gagatcgagc gcgacgtgcc tgccgacctg ccgcccgttc tggcagactt
    14281 tggcgcgctc agtcgttcgc tgcaaaactt gctggcgaac gctctaaagt atggcggcga
    14341 gagtcgctgg gtgaaactgt ctgcgcgaag tgtggagcgc gtgcggggcg gcggggcgga
    14401 agtgcgaatc aaggtggaag atcgaggcgc agggatcgcg ccggttgatc tgccgcacat
    14461 cttcgagccg ttttatcgcg ggcgcgaggt ggtggccgcg cagattcacg gcaacggtct
    14521 gggcttgagt ctggtcaaac agatcgtcga ggcgcacggc gggcgggtca gcgtgcggag
    14581 cgaaccgggg cgcggcagcg cgttcacgat tcacctgccc gccgcgcccg gtgcgcctgc
    14641 ggcggcaaac ggcaacggcc accacctgcg gcggagcgac atatgaacca gcgcattcta
    14701 ctcgttgaag atgagccggg actgttgatg actctgaccg accggctcgt gagcgaaggc
    14761 ttcgcggtgg agacggcgac cgatggcgaa gcggggctgc aacgcgcgct cgcggaatct
    14821 ttcgacctca tcattctgga cgtaggcttg ccgcgcaaga acggtttcga tgtctgccgc
    14881 gacctgcggc agcgcagcat cggcgtgccc gtcattatgc tgacggcgcg cgggcaggtg
    14941 gtggacaaag tggtcggcct caagatcggc gcggacgatt acgtgacgaa gcctttcgag
    15001 atgatggaac tcctggcgcg catcgaagcg ctgctgcgtc gagtgcctcc ggcgcacgcc
    15061 gccgcgacgg gcagtgcgca cggtagcgcg gcgggcgagg ccggagacgt ttacagcttc
    15121 ggcgcgattc acgccgattt tcgtcgcgcc gaagtgacgc gcgaaggcga gcgcatcgaa
    15181 ctctccgcgc gcgagttcaa attgctgcgc tacctgatcg agcacagggg cgcaaccatc
    15241 tcgcgcgacg aactgttgaa cgaagtctgg ggctacaacg cgatgccctc gacacgcacc
    15301 gtagatgtcc acatcgcgtg gctccggcaa aagctcgaac ccaacccgca ctacccgcag
    15361 ttcatcctca ccattcatgg cctcggctac aaattcgtcg ggtgatgaag ttccttcaac
    15421 ttttcgagaa cttcgatggc ctgtcccctg ctgagagcct gaaactcatc cggaaactcg
    15481 tcgaggctgt cgccaccttc cggatagtcg aataatgttt gcaccggcaa acgagtccct
    15541 gagaaaacgg gggcgagttg gagagagcgg ctgattaaag catagccgcg cccgcgcgtg
    15601 tcgtgaaaat tcgccaaatc tgtcgaaaac agccggaaaa aggttgaacg acgcccgtta
    15661 cggttatatc ctcgcgtttc tcgtccgttt gttgcccgcg cacttaattc catcaaggcg
    15721 aaaatttttc gggagttgtt aataaactct gtggaggagc gttttatggg tggagacgaa
    15781 gttgtgaagg agaacaacgt gccagctact gcgccccaac tgaaacccgt accccacagc
    15841 ggcgggctgg aggaactcga taccctgttt cagcaacact acgatcaggt ctatcgcacc
    15901 gcttaccgca tcaccggcag cccggctgac gccgaagacg tgctgcaaac catttttatg
    15961 cgcctcgccg gaaatacgac gaaacgcgac ctcgcaccaa gccccgcaag ttatctgcac
    16021 cgcgccgccg tcaacgcttc gctcgacctg atgcgcagcc ggacgcgtgc gcgctccgtg
    16081 ccctttgaag acgtggattc ggcgctcctg caaagtccga ttcttaaccc ggaggcgcag
    16141 cacgcggaca gtgagatgcg cgctctcatc cggcaagccg tggcgcgact cggtacgcgc
    16201 gcctccgaag ttttcgtcct gcgttatttt gaaggctacg acaatggcga gatcgccgaa
    16261 atgctcggca tgtcgcagat ggtcgtggcc gtcacgctgc atcgcgcacg cgtgcggctg
    16321 cgtggagaga ttggtgagta tctggagaaa catcatgaag cgtaataaaa aagaactgga
    16381 aacaatcctt gacgagacga tgagcgacat gcgcaacgaa caactcaaca cgcacacgat
    16441 tgacgaagcg gcctcgcgcg tctgggcacg catcgccgcc gaccacaatc cggcttccgt
    16501 gatgacgccc gccgccgagc acatcaacag ttgcggcgat tttcaatctc tcattccgtc
    16561 ttacctgcgc cgcgagttgt cgcccgcccg cacgctcctg ctcgaagacc acacgcagga
    16621 gtgcatcccc tgccgcaagg ctctgaaaga ggcgcgcacc gggcgcgctg ccgccacgcg
    16681 tgccacgctc aaacccgcac gcgcgacgca gacgaatttc acggcctggc gttgggctgt
    16741 cgcggcctcg ctcgccgtgg cggttgccat cggcgcgctc ttcttctcga accgcatgtt
    16801 cgacacgggc ggcgcgctcg cggccacggt cgagagcacg agcggcgcgc tctataaagt
    16861 gaccgacgcg ggcacgctgg cgatggccgt gggcgaagag atcggtaagg gcgtccacat
    16921 ccgcacggcg aaagactccg gcgcgattgt gcgtctcgcc gacggttcgc gcgtcgagat
    16981 gcgcgaacgc tccgagtttt ccgtctcgga gaacgggcag ggcttgacgc tgcatctcga
    17041 ccgcggcgac gtgatcgtgg aagcggccaa gcagggcgca aagcatctct atgtcgccac
    17101 gcccgattcg ctcgtctccg tgaagggcac gatcttcgcg gtcggcagcg gcacgaaggg
    17161 ttcgcgcgtc tccgtcgtcg aaggcgaagt gcacgtcaac cactcgggca gcgataaagt
    17221 cctgaagccg ggcgaccaga cgacgacggg cgcgagcctc gttcccgtct ccgtctccgc
    17281 agacgtggcc tggagccgca acgcggcgcg ctacgcgaag atggtgacgg aactggcagc
    17341 cctgcgcaaa gacatcgcgc agcaagtgcc gcaccccggc gtgcgctact cgacgcgctt
    17401 cctcgacctc gcgcccgaag gcaccgtgct ctatgccgcg ctgccgaatc tggcgacaac
    17461 actcggcgaa tcgcatcgca tcatgcagga gcgcatcgcg cagaatcccg cgctcagcca
    17521 gtggatgaaa gagcaggaaa cctcctccgc ccacgggccg gggctgaacc agacgatggc
    17581 gcgcgtgcgt gaattcggcg aatacctcgg caacgagatc gtcgtcacgg cgggcacgaa
    17641 cgcgcagggc aaccccgatg cgttcctcgt cctcggcgag ttgaagaacg cgcaagggtt
    17701 ccgcccgttc gtcgaaaagc aactcggcga actgaaaggc gtagacaagg gcgcgccgag
    17761 cgttcgcatc attgacgacc cgaacacggt cgtcgccgcc acgcccgccg caaccgcagc
    17821 agcgacgacg gctccgaaca ccgagaagaa gaccgtcgcc aagaaactcg cgggcaataa
    17881 caacaacgaa ctctacatct ggattcgtga cgatttcttc gcggcctcgc cgaagatcga
    17941 atccctgcgc ggcttgcagg cgaccatgag cgcgcccggc acgaaccgct tcgtcggcac
    18001 gcccttctat gcgcgcatcg cagacgttta caaggaaggc gcggggctga tcgtcgccgc
    18061 cgacctcgaa aaagtgatcg cgcaagcgac gagcgacgcc gcgaaagtcg gcgggcagca
    18121 gcaactcgac ctctacaagc aggccggact cttcaacctc aagcacttcg ttgtggagca
    18181 gaaggaagtc aaggcgaaga cgcagagccg cgccgtgctg accttcaacg aaccgcgtcg
    18241 cggcatcgct tcgtggctcg ccgcaccggg tccgatgggc gcgctcaagt tcatctcgcc
    18301 ggacgcgcac atcgctaccg ctttcgtcgt caaagagccg acgctgctgg ttgacgatct
    18361 gctcggcttc atggaaaccg cgtcgcccga cctgcgcaaa cgcctgcgcg aacttgagac
    18421 gcagaacggc ctcgacatca aacgcgactt cgccgcgccg ctcggaggcg agtatgcctt
    18481 cgccatcgac ggccccgtcc tgccgacgcc ttcctggaag atgatcgtcg aagtttatga
    18541 ccagaagcac ttgcagcaga cgttcgagaa cgtggtcggg aagttgaacg cctgggcggc
    18601 gcagcatggg cagaagggct tgacgctcca gcagtcgggc aacatctaca cgctgcgttc
    18661 ggtggatttc ggcctcgaag ttcactacac cttctcggaa ggctacatga tcgccgcgcc
    18721 gacaagtgcg ctgctgaccc gcgccttgca gtatcgcgag tcgggcgtgt cgctgttgac
    18781 ttcgccgaag ttcatcgcgg ccttgcccga agacggcaac acgaacttct ccgccgtcat
    18841 ctatcacaac ctcgcggcgc tgctgcaacc gcttgcaggg gctattgggg atcgctccaa
    18901 gtcgctcggc gaggaacagc agaaggccat cgccgctttc gccgcgaact ccgagccgac
    18961 gctcgcttat gcctacgcac agggcgaccg catcacgctc gcggccaaca cggagggcgg
    19021 ccccttcggc ctcagccccg gcagcctgct cggcctgccc aacgctttcg ccatgcagca
    19081 catcatgatg gaagcgatgg acgagaagaa gggcagcacg ccggagaaga agtaagtaag
    19141 cgaagcgacg agcgcgcgtc tctgtccggt ggcgacgaag ttgtcgtcac cggacagaga
    19201 cgcgcgagcg ttaaggttaa ccggcagcct gagtgaatag agaggagtct ccagcatgaa
    19261 aggcggaaat ctcattgccg gaattctcat cgtcgtgagt ctctgggcgg tcacggtctg
    19321 gttcgtcttc gcgtccggca tagccgaccg gcatgaaccg accacgcctt tgacagttgc
    19381 cgccgagcgc ggcgacgcgg ctcaggtcag gaaactgatc gccgggggcg cggacttgaa
    19441 cgagcgcgac gactccggct acacctctct cgtgtgggcg gcgcgcaacg gcaacacgga
    19501 agtggcggaa gccctcatcg aagcgcgcgc cgacttgaac gcgcgcgact gcgccgcgaa
    19561 cggctggacg ccgctcatcc atgccatcca caaaaacaat aacgagatgg cgcgcctcct
    19621 gatcgagcgc ggcgcggacg tgaacgcgaa ggccgggaag tgtacggaga aaacgattga
    19681 gagcggcgcg accgcgctgc tgcatgcggc aggcgaagac aacacggaga tcgtccgcgc
    19741 cctgctcgcc aaaggcgcag acccttacgc cgaatacgac aaggcgaacg cgctctcgaa
    19801 ggctgtcgcc ggggcgatgg acttctcccg tccggacgac aaacagtgtc cgaccgaaac
    19861 cgtgaagact ctgcttgaga gcgcgcccga actgaccacg aagcgcagcg tctgggatcg
    19921 caagtccgtc tttatcgcca ggcacaaggg ctgcacggaa ttagtgagcc tgctcgaaca
    19981 gcgcaagagc gcgttggccg caaaggccgc cgccaacaat tgaattgtga tccgggcttg
    20041 agcgtgcagc cggtaggatt cgccgccgca ggtacagagg acacagggaa aaaatgatga
    20101 agcaggaacg gtgaacgaag aacgaaagca actgctttgg tcttcaattc agcattcatc
    20161 gttccgcgct catcgttttt ctttgtgctc tcggtgcctg cggtggcgcg tgcttttctg
    20221 ctcacgcatt tcactctggc gcatcttctt tctattcaaa aacctttctc acaagtcgaa
    20281 ctttaattca catcgaagcg ttattcttca acggaacaca cgcggttgtc ggcgttgaat
    20341 tcactcccga ttctgtttgg agttgccggg agaatatcgt ataaagcgaa ctactctgct
    20401 tgtgtagttc gcgcttctca actttacttt gtctcaaact gaaagccgcg acggcttcaa
    20461 ctcattatga atccagtcat cgatctagac ggtttgggcg tgaccttcgg cgggcgtccc
    20521 atcctacagg acttgcgcgg cacgctcagc ggtcgcgcca tcggcctgct cggtccaaac
    20581 ggcgcgggga agacgacgct catccacacg ctgctcggtt tccatcccgc gaccgctggc
    20641 acggcgcgca tcttcgggca ggacatctcc gccaacgcca aacagattcg caccctgatc
    20701 ggttacatgc ccgaacgcga ttcgttcatc gccaagatgt cgtgcgtgca tttcgtgcgc
    20761 ctcatggcgg aactctccgg catgccttcc gacgccgcgc tcgaacgcgc gcacgagtcg
    20821 ctcttctatg tcggcctcgg tgaagcgcgc tatcgcacgc tcgacaccta ctcgctcggc
    20881 atgaagcaac tggcgaaact cgcgcaggcc atcgtccacg ggccaaagct catcatcctc
    20941 gacgaaccca cgaacggcct cgacccgccc acgcgcctgc gcatgattcg tctcatccgc
    21001 gagatacgcg acagcggcga ggcgcgcatc atgttgtcgt cgcacctgct gcgcgacgtg
    21061 gaagaatgct gcgacgagat actcgtcctg aaggacgggc gcatcgccgc taactgtaat
    21121 ctggaagagg aacgcaaggc caaccgtaag tttatcgaac tggagatgcg cgggggcgac
    21181 aatcgcgcct tcgcggaagc catcggccag ttgggctgcg aatacgcgtt cacgaacgag
    21241 cggcggctga aattggtctt gcccgaaggc atcgagatac gcgacctgta ccgcgtcgcc
    21301 gccgggcagg cggtgcagat acggcgtctg aactacaagc gcgattcgct ggaagacatc
    21361 ttcctgaaag cgatggaagg cggcgcggtc ggcacgggag gtaacggcaa tggcagtcta
    21421 tgagcatggc tacaagccat atacgggcgg gttgacgccg gaatggtcgc gctttttgat
    21481 catcccgcgc cacgcttacc gcgacgtgtt tcgttcgaaa ttcttcatcg cgttcttcgc
    21541 gctctgcttc atctacccgc tcgtggccgc gatcctgatc tacctgcacc acaacgtcaa
    21601 tgccatcgcg ttgatgcaga ttaacgtccg cgaactcgtc cccatcaacg cctctttctt
    21661 ccacgtcttc atgacgacgc agtgctcgct cggcttcttt ctcaccgtgc tgatcgggcc
    21721 gccgctcgtc tcgcgcgacc tcgccaacaa cgccctgccg ctctatcttt cgcgtccctt
    21781 cacgcgtaag gagtatgtcg tcggtaagat gagcgtgatt ctggttctgg tttcgctgat
    21841 cacctgggtt cccggcctcc tgctctttat cttgcaggct taccttgagg gcgcaggctg
    21901 gttcgggaac aacctgtgga tggcgggggc gatctttttg agcgcgtttg tgtggatcgc
    21961 cgtgctctcg tttctgtcgc tcgccgtctc tgcgtggttg aagtggcgtg tcgtggcgag
    22021 cggcgcgctg ctcgcgatct tcttcatccc gtcggccttc ggcgagatcg tcaacgcact
    22081 gttcatgacg cgcatcggcc atctcatcag tatcggcgcg atcatgaact cgatctggct
    22141 cggcctcttc ggattgttcc aacgccagac cggtcgcatg gagacgacgc aaggcggacg
    22201 gcgcatcgaa atggtcttct acgaaccgcc gctctggtct tcgtggctcg cgctcggcct
    22261 gatctgcgcc ttctgcgtgc tgctcttgat gcgcaaggtg cgcgcttacg aggtggtgaa
    22321 atgacgacgg caccggcaac agtagcgaca aacgcaaacg tcacggcgca cgccgtggag
    22381 agaatgatcg tcttcgaaga tgtctcgaag ttctacggcg agatactcgg cgtcaatcgc
    22441 gtcaatctct ccatcgcgcc gggcatcacg agtctggtcg ggccgaacgg ttcgggcaag
    22501 accacgctca tgaacctgat gacgggactc ttgaagccga cgcgcggcca catcagcgtg
    22561 ctcggcacca gcccgcacaa cccgcaggaa ctttttcgca aggtcggcta ctgcgcgcag
    22621 ttcgattcgt ttccgcgcgg cgcaacgggg cgtgagttca tagagttcta cctgcgcacg
    22681 cacggcggca cgcgccgcgc cgtggagaat ttgacgtatc aggctctcga acgcgtttcg
    22741 atgctggagg cggcggatcg caaggtggcc ggttacagca aaggcatgcg ccagcgcatc
    22801 cgcctcgcgc agtccatcgc gcacaatccg gcggtgctca tcctcgacga gcctctgaac
    22861 ggactcgacc cgatggcgcg cgcagagatc atacggctct ttcgccaact cgccgccgaa
    22921 ggtctgtacc tgatcatctc cagccacatc ctgcacgaag ttgacatgat gtcggacagc
    22981 gtcgtcctgt tgaacaacgg ctacgtcgtg gccgaaggca acgtgcacgg cgtgcgcgac
    23041 gagatcgaag agcacccgat gcagattttg attcgctgcg accgcccgca ggttctggcc
    23101 gcgcgcgtct tcgagcagga cgacggcgcg gtggtcgaag cgcgcctgca cgacgaccgg
    23161 caaggtctgt ttgtgaagac gcggcgaccg gacgatttct atctgctctt gaaccgcgtc
    23221 gtcttggacg gcgacctaaa gatcgaatcg gtcgcgcccg cagacgacga cctcaacgcg
    23281 gtctatcaat acttgatcag ttcggacgga gggcgataat gagttcgacc attgattcgg
    23341 cgacacccgc acaaattaag agcgcacgcg aaatgccctg gcaactctgg gcgcagcaga
    23401 cgctcgccat cctgcgcctt gagttgcaga aaaactttct gagccgtcgc gcgctgttga
    23461 tctacctgct cgcagcgttg ccgatcctgc tgttgttcgt gctggcggtc ttgccgccct
    23521 ccgaagacga cacctttaac aacttcgccg agttgggcgt cctcttttcc gccatctaca
    23581 acgggctcat cctgcgcacg gtcgtcttct tcggctgcgc gtggatcttt atgaacctgt
    23641 ttcgcggcga agtggttgac cggagtttgc actactactt tctctccccg gtcaagcgcg
    23701 aggtgctcgt cgtcggcaag tacctgtcgg gactggtgac ggccatcgtg ctcttcgggc
    23761 tgaccacgct cgtctcgctc tttatgctct acttcccgcg cttctcggcg gaaagctcgc
    23821 gctacttttt cggcggtccg ggcggcaagc aggcgctcat ctatctcggc atcacgacgc
    23881 tcgcctgcat cggctacggc gcgatcttcc tgatcgtcgg actcttcttc cgcaacccga
    23941 tcattccggc gatcctgatc tacggctggg agtggatcaa cttcctgttg ccgccgctcc
    24001 tgaaaaaggt cggcgtcata cactatctac actcgctgat gcccgtgccg atgaacgaag
    24061 gaccattcgc cgtcatggcg gagccgacgc ccgcttggat ttccgtcccg agtctcatca
    24121 ttttcaccat catcgttctc atcctcgccg gactccacat tcgccgcatg gagatcagct
    24181 acatcggcga ctgacgcgtg cggtcggtgg ttgagcggca tttaagattt gcggcggtta
    24241 agattttaag actcgccaca gagacacgga gaacacaaag ggggcacaga gagttcaaat
    24301 attctctgtg ccccctttgt gctctctgtg tcccctgtgc cccggtggtg aagctttatc
    24361 cctgcttcct catcctgaga cttcaatctt ccagacatct gcctcggcct gcccggcctt
    24421 gatctcaacg tcgagaaaca tccggatgac ttcgatgttg gtcgtcgtgt gcagcgtggg
    24481 ctggccggtc gtgtatgagc cgccgcccgc gagcgcgaga gggatgagaa gttgatcggc
    24541 taagtgttcg ccgacgggcg cgcccgtttc gagatacgcg gctgcttcat ccgatgcccg
    24601 ttcggcaatt gtctcggcgc gcacgccgcg ctcgccgatg cccgtaaaga cttccgtgag
    24661 atgttcgctc tcgatctcaa gcgtcaggac gttacccgga ctgtgcgcgt tcgtcgaagt
    24721 ctcgacgcgc aactcgtcgc cctcttcctc gttccatccg agttgttcac ggacgaccgt
    24781 taactcgcgc tcgccgaccg tgggaggcag cttgacgacg agagcgcgtg cgcggcgggc
    24841 gcgaatttcg cggcgcgtga gcagttcgag cggcgcgagt gcgccacgcg cgacgggttc
    24901 gatgcggacg ttgatgcgac cgccgcccgg cggataaaag ccgtagcgtt cgagttcggc
    24961 agagacgcgc acgcccatgc ggttgatgag cgggagaaat gctctggcga tgaactcgta
    25021 gggcggcgcg tggatgttgt gcgtgccgcc ttcaaaggcg agcgtgctcg gcgcgtccgc
    25081 gatcatcaaa ggcggcagga tcgtttgcag cacgagcgac gtactcccgg ctgtgccgat
    25141 ggggaaggaa tagtcgccgg gcttgaccgt gccgggcgta aaggtgaacg cgcttgagcc
    25201 taacgatgcg ccttccacct gcgcgccgcc gatggcagcg gcggcgagaa cggcggtgag
    25261 atgctgtcgc tgcaagccgg gcttctccct tcgtgcgcgc acgttgtaga tgcggaaagg
    25321 ttttccggtg atgagcgaga gcgcgagcga ggtgcgtatg atctgcccgc cgccctcgcc
    25381 ttgtgaaccg tcaatcgtca gcatgatgag gcgattgtaa cccgaacgcc gtcgcctttt
    25441 cacatcctct tttcacagtg cgcgtccgcc tgttgtactc tggcagttgt gactgtggcg
    25501 aaatcaatcg tgctgcgctt cgacgcgggg acgctcctgc tcgacggact tgagcgcact
    25561 ccgccggtgg cgggcgacgt gccggagagc tttcgctggg acgagcgcgt ccggcgctgg
    25621 cgcgcgcccg cgtcggctta tcgtcatgtc gtgcgcgaac tgatccgcaa aaaagtggcc
    25681 ttcgaagatc aggcgcgcgc ctatcacaag ttcgacttcc agacgaagtt cacggtcgag
    25741 ccgcgcccct accagcagga agctatagcc gcgtggcgaa aggcggagcg ttgcggcgtc
    25801 gtgatcctgc cgacgggcgc ggggaagagc ctcgtcgcgc agatggcgat tgaacagacg
    25861 cagcgttcca cgctcgtcgt cgtgcccacg attgacctga tgaatcaatg gtacgacctc
    25921 ctgctctcct gctttcaagc cgagatcgga ctcatcggcg ggggcttctt cgaagtcggc
    25981 gcgttgacgg tgacgaccta cgcctccgcc ttccgtttca tggaacggct cggcaatgaa
    26041 ttcggcctcg tcatcttcga cgagtgccac catctgcctg gcgacgtttt tcgttacgcg
    26101 gcggagatgt cgctcgcgcc tttccggctc ggcctgaccg cgacgcccga acgcgccgac
    26161 ggcgcagacg ccctgctgga agagttgatc ggtcctatcg tctatcgccg cgaagcgcgc
    26221 gaactcgccg gggaatacct ctccgattac agcatcgtgc ggctgcacgt cgagttgagc
    26281 gcggcggaac gcgcgggcta cgaggcgtcg cgcgccgtct tcaaaagttt cctgcaagag
    26341 aagaacatcc gcctgagcag cctgcacggg tggcagcttt tcatcgcggc gagcgcgcgg
    26401 agcgaggtgg gacggcgcgc gatgatggct tatcgagagt cgaagcgaat cgcgctcggc
    26461 acagaggcga agttgcgcgt gctcgccgaa ctgttgaagc ggcacaggcg ggatcgcgtg
    26521 ctcatcttca ccgccgagaa cgagatggtc tatcgcatct ccgaacaatt cctgatcccc
    26581 gccatcacgc acgagacgaa catcaaggag cggcgcgaat ggctcgccgc cttcaacgcg
    26641 ggcgacgtgc tcgcgctcgc cacttcgaag gtcttgaatg aaggcgtgaa cattcccgcc
    26701 gcctcggtcg ccatcgtcct ctccggctca ggttcgacgc gcgagcacat ccaacgtctg
    26761 ggtcgcatac tgcgcaagca gccggagaag gaggcgacgc tgtacgaagt cgtcaccctc
    26821 gacacgaccg aagagggcat cagtcgccgt cggggcggag aacagtttcg cgcgggcaca
    26881 gggcgcagcg aaggcgggcg tttgcccggt gggttgtaat ctatgttgac cgcagacctc
    26941 gcactaagct ggcagcgcgg cgcgcagatc aagccgcgct acattgacgc cgaagacgcc
    27001 gactggctgg acgcggcttc cgccctgatc gaactcttcc gcgagcatcg aggctgcacg
    27061 cgcgccgaac tcgaagcggc tctggaagag tacgtcggcg cggggacgga ctacaaaatc
    27121 ctgcgcggcc tgatcaagct gttgacggat cgcgcggagt ttgcggcggg cgtcgaagtt
    27181 gtcccggcag atttgcggcg cgcggtcttt ctcaaagcgc gcggcgcgca tcccgtcacg
    27241 gacgagagcg tgcgcgcgca agtcctgacg gaagccgggg aagaactcgg ttgtgcgacc
    27301 gaactggtgc gcgcgaattt gtatgcggac ttgccgaaga accagcggct cgtcgagttt
    27361 gaagagacgg acgcgcgtgc gctcctcgaa ctctacaacg tggcgcaggc gcaggcgttg
    27421 ctctatcgct gcgtgcggat gcgtctcagc atcgagccgc agggggtgga aaactatcgc
    27481 gaactcttcg gcgcgatcaa agcttatcgc ttgattcaca cgatcaaggg gagtccggcg
    27541 cgcggctatg agatcgaact cgatggtccc gtctcgatgt ttcatcgttc gcaaaagtac
    27601 ggcatccaga tggctgtgtt tttgcccgcg ctgctcctgt gcaaaggatg gcggatgcgc
    27661 gccgagatcg agtcgagaca cacgggcacg tcgttcttcg agatgacgag cgaggcgcac
    27721 ggcctgcgtt cgcattattt gagcgtgacg ccttacgaaa atccggtgag ggaaaagttg
    27781 ttgcagagtt gggcgcgctt cgagagcgag tggacgttgg agacgagcgg cgagatcatc
    27841 gatctcggcg caagcgcgtt catccccgat ttcgtactgc ggcacgcagg ggggcgcgtg
    27901 gtctatctgg agattctcgg cttctggacg ccgccgcacc tcaaggagcg tttgcaggag
    27961 tttgccggga gcggcttccg ggatttcctg ctcgccgcgt gggaggaact gcgcggcagc
    28021 cgcgagcctc tggtcgccgc cccgccgcac acgctcatct tcaagcgttc gctcgatccg
    28081 gcgacggtcg agttggccgt caacagattg atctcggaag cgggtcagac gcaaggcggc
    28141 gaactggcat gaaaactttc aatctctgct ttctcggttt cggtaacgtg gggcgcgcgc
    28201 tgttgcgact gctcggcgag aagtccgtag cgttgcgtga agagtacggg atcgaatggc
    28261 gcgcgacggg cgtggcgacg cggcgcatgg gctggatcgc gtcggcggat ggattgcctt
    28321 ccgaatcgct gctggcaggc gaggctacgg tgcgacgaga aggcgagtcg cagcccgcga
    28381 acgtgcgcga gtggctggcg gctgcgcgtt gcgatgtgct gtttgaaaca acgtcgcttg
    28441 agccgctgac gggtcagccc gcgattgagc acgcgcgcgc cgggttggat gcgggcgcgc
    28501 acgtcgtgac ggcgaacaaa ggaaccatcg tgcatggcta tcgagagttg gacgcgcttg
    28561 cgcgcgaacg cgacagacgc tttttgtttg aagcgacggt ggcggacatg cctgtcttct
    28621 ccctctttcg cgaatgtctc ccggcggctc gcctgctcgg ctttcgcggc atcttcaact
    28681 cgacgacgaa catcattctc gaagagatag aggcggggcg cacgttcgcg gaaggcgtcc
    28741 ggcgcgcaca actcctcggc gtcacggaga ctgatccggc ctacgacgtt gacggctggg
    28801 attcggcggt taagacgtgc gcgctcgtga atgtcttgat gcgcgcacct ctgagactgg
    28861 atgagatagc gcgtgaagga attcgcgaac tggatgcggc gagcgtgcgc gcggcgcacg
    28921 cggcggggaa gccttacaaa ctcgtgagcc gtgccgaact tgaaacgggt ggccgcgtgt
    28981 cggcgcgcgt gcgcgcggaa caggtcgcaa cagacgaccc tctggccgtt gcgggcggca
    29041 cgtctctctc catcaatttc gagttggata ttcttcccgg cctgacgctc gtggcgcacc
    29101 aacccgattt gcagagcacc gcctacgggt tgctcgccga tttcgtcaat gccgtgcgcg
    29161 ccgaaacata gggaagggaa tttagacgca agaaaaattc cctctgcgct ttgctttgcg
    29221 tctccggcgt gagatcgatt tctcgccgga ggcgcaaagg aggcagaggg agaggcaggg
    29281 agaggcgttg cgttcttggt tatcgtgcgg cttactttta gtcggagtcg gggacgatga
    29341 aggcgaggtt ggcgagcacg ctgcgcaggc gaacctgtcc gagcagggtt tcgaggtaac
    29401 acgccagacc ttcgagcgcg gggccaatct ccagaactct acgcgccgcc gccacctttt
    29461 taacgaagcg ttcgtactcc tcccaatcct tgtacatcag gtagcacatg ctgccgtcgc
    29521 gaaaacgctt gaggtgcgcg agcaggagcg cgaccgaaaa ttgatcgcat tcctgatcgg
    29581 cgcggcgcgc gagttggacg agattccaga ggtcgcggta gagaatgagg gcttcttcgc
    29641 ggcgggcgtg aaagtcgttg aagagttctt cgccgtcgag cgcgggattg aagacctgcg
    29701 tgagcgagat ggttgattgc tggaagcagt tgcgcagcat cccgtgtgcg cgttcgagct
    29761 tgccgcgaat aggcggcgag tgcgggtcgc tgatgccgac gagctcgaag ccgtagacgc
    29821 gcccgatctc gtggccgatg gcataactga tgccgtcgag cgtggagagc aactcttcgt
    29881 ccgcaccatc catgctgagg atcccggctt cgatgttttc gatgagcgcg agcgactcct
    29941 tgtggacatt gttaaagacg gagaaggttg aggcgagcag ttggtcttgc tgcaccttct
    30001 tttcgatgag gcgcaggtag tcgagcagac gtgcgagatc gacgaagaga cggcgcatcg
    30061 tctctctaaa ggagccgggc gtgaggctct cgacgagcat ggcgagcggc gcggacagag
    30121 ggacttcaac cgtcggaagc agaaacgtct caaggttgat ctcttcgccg ctgctcgctt
    30181 cgctggcggg aagataggcg agagactgcg gcgtcggctc gtgtgcgggc tgatcaaaaa
    30241 tagcactgct catagttgat gtgtcctggt ggatatgttc tgccggtgcg caaccgccgg
    30301 acggctggag acggagcgtt gcctttaaat tactgtcggt caggcttcct gcggcgaaaa
    30361 agcgtgcgcg aagaagagtc tctccgcgcc agacctctaa ctgtgctgtc aaactttatg
    30421 ccagaaattt cttgcgaccg gcgacgcgcg aaatcgcttg atttgcgacc atttcccgca
    30481 tccgaaagat tgccgggcgt gaatttcagg caggccgact gacagtttga caggtcaagc
    30541 tggcagtgct gcgacaatat gacaggcgcg cacgcgcacg ccgttttccg tgggagcaat
    30601 tattggctat agttgaccca tactattttg cggatagaca ttatgccgga gacgcgcgcg
    30661 agatgctttc gtgcacgcca gcgctctggt aagcctgtgc ggcgggcggc gggcacggcg
    30721 tttgaggaga ggaaggcagg gacgatgatt aacgtgcgca gagccgccga gcgcggccac
    30781 ttcaatttcg gttggctcga cacccgacac acattctctt tcggcgaata ctacgatccg
    30841 cgcaacatgg gatttcgcac gctgcgcgtc atcaacgaag atcacgtgcg cgccggacac
    30901 ggtttcccca cccacccgca ccgcgacatg gagatcatca cctacgtgct cgaaggcgcg
    30961 ctcgaacaca aagacagcat gggcaacggc acggtgatac tccccggcga agtgcagcgc
    31021 atgagcgcgg gcacgggcat tcgtcacagt gagaagaatc attcgacgga acaggacgta
    31081 cacttgctgc aaatctggat catcccggag gagcagggat tgacgccgag ctatgaacag
    31141 aagatgtttg aagcagacga gaagcgcgag cgtttgcgcc tcatcgcgtc gaacgacggg
    31201 cgcgacggtt cggtcacgct gcatcaggac gcgagtttgt atgccgcgtt actcgaaccg
    31261 ggtcaggagg tgacgtatca actgtcgccg gggcgacatg cgtgggcgca agtggcgcgc
    31321 ggcgcgttgg agatcaacgg gcagcttttg cagcagggag acggggcggc gatcagcaat
    31381 gaatcgcagt tgaagattgc cgggcgcgac tcatccgaag ttctgttgtt cgatctcgga
    31441 taaggcgcgg gcgcgtgtgc gaataatctg tgacgtgagc aaaaagtctc agacgagggg
    31501 cgagagttta aatctctaac acgagcgggc gcggagtagt cgccactact ccgcgcccgc
    31561 ttaacttctc tttagcgtcc ggcgcgtcaa gcaatgtccg gcttagcgcg tgtgacgaaa
    31621 ctgttttcgc tcaggcgcga aggcttctgt tgcctgagga gagtgcgaag tttcatcctc
    31681 gccttgtgca gttgcgactt gctcgtcccc accgaacaac ccaacatccg cgcaatctcc
    31741 tcgtgctcgt gcccttccac atcatgcaac acaaagacgg tgcggtagcc cggcggcaac
    31801 tgtccaatcg cacggtctaa agcgattcta tccaccaccg gcatgctgtt cgggttctgc
    31861 gtccccgcca caatctgcac gggcgtctcc ccgtcatcgg tggtctgctc taacctcacg
    31921 ccgcgcttgc gaaagtgcat caagacctga ttgaccgtca ggcgatggag ccaggtggta
    31981 aaagccgact cgccgcgaaa tgagccgatc ttgcgaaaga gttggatgaa ggcttcctgc
    32041 gccaggtctt cggcttcggc
//
LOCUS       DASWNS010000035        19027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_209048, whole genome shotgun sequence.
ACCESSION   DASWNS010000035 DASWNS010000000
VERSION     DASWNS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19027
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209048"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3495)
                     /locus_tag="VGB76_01155"
     CDS             complement(<1..3495)
                     /locus_tag="VGB76_01155"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEY0097533.1"
                     /translation="MKQFPAPRSIPVSRRAVAFIAILLSTFVLLTAPFFWLRSLAAAP
                     LSTAELKAASAQADKARSLLAPPGDLDTTFGNAGKVVTPFPNGLDAAEDVAVQADGKV
                     VVAGGSGTDFAVARYNMDGSLDQTFGTGGRVNTDFPIGEGGFASALVIQPDGKLVLAG
                     SANSPEDDFNSAFALARYNPDGSLDTSFDSDGLVVTPFTGASATANALALQPDGKLVV
                     AGSSRNGTTFESVFALARYNTNGSLDTTFDTDGRLTIDFAGVTFEEALGVAVQADGRI
                     VAVGLGSSVFAVARVNTNGSPDNTFDLDGLVTTDFGGSFEQANAVAIQTDGRIVVAGE
                     IATSVAADDNFALARYNTDGSLDTSFDGDGKVTTDFDLVDGARDVLIQPDGRIVAAGR
                     AGSIPTGTFFAVARYNTNGSLDNSFDTDGKVLTDVAGGRFNLGGRGVALYTDGRIVVA
                     GGDNTSGQLDFGVVRYNTNGSLDNSFDTDGKVFTDFPLNEETIEDMALQPDGKIVAVG
                     RMNTRVIIDDAQDFNFLLARYNPDGSLDTTFGSGGLVSTDFTGNGDDFARAVVLQPDG
                     KIVVAGQTGPQFSIVPSADTSFGVARYNANGMLDTDFGTGGRVTVDFGTGHDGASDVA
                     LQPDGKIVLVGGDFLNFKLARLNANGSLDSGAAGDSTPADMFGTGGKVTTDFNERSDA
                     AHAVVIQPDAKIVAAGSSSGDFALARYNADGSLDTTGFGTGGKVLTDFSAGGDGINAL
                     VRQADGKLVAAGGATISGGNSDFALARYNTDGSLDSGTGTDSTPGDSFGTGGKVTTDF
                     GGFDSANDLALQADGKLVAVGLYFPLSVVTTAEGRVQPRATDDVEIYKTPSDAAKSGR
                     QAKPAALVNGQAQPTARAAAFSFFALARYNTDGLLDSSFGAGGQVTTDLFGIENEAAA
                     VLIQADGKIVAGGYTDTGSSRDFALARYKAAEDPTPTPTPTPTPTPTPTPTPTPSPTP
                     TPHTVELSASAYVVDENARSVLITVVRTGDTAAPATVDYETEADSVTVGCNVKNGFAS
                     ERCDYTTTLGTLRFAAGETSKTFRIFITDDVYLDGDERFNVTLSNPSSGLTIGTIFSA
                     VVVIVDNDTNPNAPNPVDSAQFFVEQHYVDFLNRQGEPTGVSAWVNALNNCPAGNQSC
                     DRVGVSSAFFRTDEFQFKG"
     gene            3823..4290
                     /locus_tag="VGB76_01160"
     CDS             3823..4290
                     /locus_tag="VGB76_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY0097534.1"
                     /translation="MLSLILLQAANAITDATAATTATAAAPVAQVGSPVTAYWPVMIF
                     FVVAVVFPVLPIVLGRLVRPSIWQKDKMRPYECGIDPTTEAHDRFTVRYYIVAMLFLV
                     FDVETIFLLPWAIIFMGDDFSFTKFALVEMLVFIGILVLGYYYAWRKGALEWA"
     gene            4406..5050
                     /locus_tag="VGB76_01165"
     CDS             4406..5050
                     /locus_tag="VGB76_01165"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY0097535.1"
                     /translation="MAEHPEGIVLTTVDAIFNSLRRNVAAPVMNVKPVRNTVNWARKS
                     ALWPMTFGLACCAIEMIATGAGRFDIDRFGAGVFRPSPRQSDLIIVAGTVTLKMGPVV
                     RRVYDQMPEPKWVIAMGACASTGGPFDSYSTMQGVDRTIPVDVYIPGCPPRPEALLYG
                     LMRLQDIIMKEAPSAYIFDSDGTVHRQRQLEGDGEEDARLDAEREDDEVSTVAG"
     gene            complement(5179..5826)
                     /locus_tag="VGB76_01170"
     CDS             complement(5179..5826)
                     /locus_tag="VGB76_01170"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0097536.1"
                     /translation="MESTRVLLIRHGQSEGNAARRFGGHTPTPLSALGHRQAEATARA
                     LESEGVTAIYSSDLLRAVETATPLAAALNLEIRQSDAFRERSIGRMEGLTFEEAAQKF
                     PEEYAALLRRDFEHVLPGGESYRQLLDRAAHALDRAINANRGGTIAVFSHTGTICILA
                     LHLLGALDAPTLRPVWLATANCGISRFTLRDDGFLRISALNDTRHLGDLSPDAKA"
     gene            complement(5990..7069)
                     /gene="prfA"
                     /locus_tag="VGB76_01175"
     CDS             complement(5990..7069)
                     /gene="prfA"
                     /locus_tag="VGB76_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY0097537.1"
                     /translation="MLEKLEQIEARYEALSEELYSPELLADQAAYAKAAKQHRSLGDM
                     VKRYREWKTIKEELAGAREMFDMNADDEEMRELARVEVETLQVQLERCEEDLRLLLLP
                     TDPNDEKNVILEIRAGTGGDEASLFAAEMLRMYGRYAERQGWRMEILNTSESGIGGYK
                     EATALIEGDKVYSKLKHESGVHRVQRVPQTETSGRIHTSAITVAVLPEAEEVDVKIDA
                     KDLRIDTFCSSGPGGQSVNTTYSAVRITHLPTNTVVSMQDEKSQIKNREKAMRVLRAR
                     LQEQEEQKQHELLASERRSQVGSGDRSEKIRTYNFKENRVSDHRIGLTVHQLNLVMEG
                     QLDEIINALVTHYQAEKLKAETVAA"
     gene            complement(7206..8414)
                     /locus_tag="VGB76_01180"
     CDS             complement(7206..8414)
                     /locus_tag="VGB76_01180"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEY0097538.1"
                     /translation="MSANEKDLIVGGQAVVEGVMMRTPNAYAVAVRRADGEIVFTGAT
                     LPKWSDKYRFLRLPVLRGAAVLVQSMALGIKALNYSASEAFADAEQEQEVASVALTPA
                     VVEGEGDFAGITGGVPGLIPIPTERRAEDEMKRGANASVIGSIVFALFFNVMLFIVAP
                     LVLTNALFIAAGWAVPAGMNANAAMAQTQSVANTTTGASATNQTATSEQAWYARAWGG
                     VRGYLHPVRPSVAFNLVDGVIRMSFFLIMIFSFSLLKDIRRVFEYHGAEHKTVFTWEA
                     GLPLTVEHARTQPRQHPRCGTSFLMVVMLVSIVLFSIVKFDSLALNMLTRLALMPVIA
                     GLSYEIIRLSARREGSLIFKMITLPGIWLQNITTKEPDDKQLEVALYALKESLKLEPR
                     PQEATMTPLS"
     gene            complement(8650..8895)
                     /gene="rpmE"
                     /locus_tag="VGB76_01185"
     CDS             complement(8650..8895)
                     /gene="rpmE"
                     /locus_tag="VGB76_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY0097539.1"
                     /translation="MKDGIHPKYNEIKVVCACGENFTTRSTRKDDLHVEICSACHPFF
                     TGKQKLVDTAGRVDRFNKRYGKKTETAPAATSEQAAS"
     gene            9182..10456
                     /locus_tag="VGB76_01190"
     CDS             9182..10456
                     /locus_tag="VGB76_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY0097540.1"
                     /translation="MIDETDKTQSDDSANRATDDASAPLRPDDRSPSPDGEISRAGSL
                     PAPPHADAPPAPESQAAPQAKAPSGQSRDTVMGASTGDASNAAPDAADAPPADETPEQ
                     KRARIIAEAKAKAAAKMAAAKGNATTAGGETAAPKAKPPGVAPSATSDEEKAARIAEA
                     RAKAAAFKQAAGESAGANKTPPAPFAAAGAPAAPAASAAGAPKAPVKKKEEGAKPADA
                     SQHPLVKRLREGLDGVVIEATEFLGQLSVRVAPARIVEVCEFLKRDAESPFEYLSDLT
                     CVHFPENAGAPFEVVYNLYSISANVRVRLKAETTEAAGIESVTGVWPAANWLEREVYD
                     LFGVRFHNHPDLRRILLPPDWEGHPLRKDYPLEPVENNWTAKHLPEFSDVQREQLEQR
                     RAYGLEALSTPDERRVREIFRAGKEVMPLDRK"
     gene            10569..11702
                     /locus_tag="VGB76_01195"
     CDS             10569..11702
                     /locus_tag="VGB76_01195"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY0097541.1"
                     /translation="MHQVEVATTQETLLDSPEMVLNMGPQHPSTHGVLRVILKLDGET
                     VIDLDCDIGYLHRGMEKIAEHRLYPMIAPYWDRLDYIAAVSNGLLWVETVEQMLGLEV
                     PPRAHYIRTVLTELNRISSHLLWLATHGLDIGSFTVLLWALREREEILKIFERQFGAR
                     LTCHAWRIGGMPNDTYDGFTEDVTQFVDNFPHRLEEYETLLSENRIWLGRTVGIGVIS
                     AEEAIAIGLSGPALRGSGVAWDIRKSRPYAAYADMEFDIPVGENGDTYDRYLVRLEEM
                     RQSRRIVQQALAKMPDGPVNAKVPKIIKPPVGDYYHSIEGPKGEIGCYLVSDGSSRPA
                     RVRVRPPCLINLQALRQMCLGHLVADVVAIIGTLDIVLGEIDR"
     gene            11815..12264
                     /locus_tag="VGB76_01200"
     CDS             11815..12264
                     /locus_tag="VGB76_01200"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEY0097542.1"
                     /translation="MRLAVQEMTRERFAPFGILLDSRGSVEIDLGMGAPSLTGATSER
                     RAFEFQFMARHKRTMQVFSPLASSRSIICVAPPTERDAPALDEVVAFLVNGKLPYAYH
                     KGTWHTPPFAVGEWASYLVVDRAGTLDSDYELVDLKAVHDCTFEIDV"
     gene            12313..12582
                     /locus_tag="VGB76_01205"
     CDS             12313..12582
                     /locus_tag="VGB76_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097543.1"
                     /translation="MSTDRTTEILNYLSAISRDVGELRTEVHSLRDEMSARFSEVNTR
                     MDRIETEVTRLGREQRLLNRIGSVMLETRADIEELQERVDVLEGK"
     gene            12609..13940
                     /gene="nuoH"
                     /locus_tag="VGB76_01210"
     CDS             12609..13940
                     /gene="nuoH"
                     /locus_tag="VGB76_01210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY0097544.1"
                     /translation="MLALFAPPDLINGLLLKLGLGRETINYVVWPFIQIGAVATVLAG
                     WAAYASYLERKISAFMQARLGPMRVGPWGLLQPIADAIKLLTKEDLKPDRADTYIFFY
                     APFISVVAAFVVFVVIPFAPDWGVITDLNIGLLFVLSVSSVGVLALILAGWSSNSKYA
                     LLGGLRSSAQMVSYEVAMGMALIGALMFSRTLSLSGIVSAQGLDSIWYIFYQPLGFIV
                     FLISGIAENNRAPFDLPEAESELVAGFHTEYSGMRWSLFFMAEYAAMIVVSAIATAVF
                     LGGWYFPFVWRLSASYPQLYVAVSLLVFILKTAFLLYVYFWLRWTFPRYRYDQLMDLG
                     WKWMIPAALLNITLTAIAIFTVQALDGWRGMDTVESIARGVNLTRIGKAIMIAFGALG
                     VFIAAGMLSYINHGTRDFNLKKQRRGIQLVNPPQGQTAVASTTTAAVASEG"
     gene            13956..14489
                     /locus_tag="VGB76_01215"
     CDS             13956..14489
                     /locus_tag="VGB76_01215"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEY0097545.1"
                     /translation="MPEVYVPEATWKEKLKRFFLIDLIQGLKLTLKFNVGALTDKDSV
                     AGKGIYTEQYPKVRAEVAPRFHGAPRLNMDPETYETLCIACNLCALACPEDCIDVIPM
                     DIEIVTAGKARKKKVLDEFIFDTSRCMFCALCQEACPTACLELTQEFELATYSRAGFV
                     WNREMLEQGRETVKYEK"
     gene            14572..15219
                     /locus_tag="VGB76_01220"
     CDS             14572..15219
                     /locus_tag="VGB76_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY0097546.1"
                     /translation="MTLSGWEAVFFWVFAIAALVAALLTILARNAVHSALFLISTLIS
                     VAALFILLGAEFIAGVQILVYVGGVMVLFLFVIMLVNVGAEERGGEMLFNRPAQVATS
                     AIFLVLLSLGFIYAIRQGYEGLLIRDNLDAPAQRAQLAERRNAEQQNLRLAATGTSRI
                     STDTQLVGASLYRFAALPFEIASVLLLVAIIGSVMLARTAKQEEATDDIETKDAG"
     gene            15321..15728
                     /gene="nuoK"
                     /locus_tag="VGB76_01225"
     CDS             15321..15728
                     /gene="nuoK"
                     /locus_tag="VGB76_01225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY0097547.1"
                     /translation="MNNFIASALAFVLQEAVPGRWQMIKQGVLEPDLSKFLVIAALLF
                     VIGVAGVLTRRNIIVIFMSIELILNAANLNFIAFSRYLQDTGKMNAVAGQVFTIFIIV
                     VAAAEAAIGLGLVIALYRNKETIFIDKIDLLKW"
     gene            15826..18504
                     /gene="nuoL"
                     /locus_tag="VGB76_01230"
     CDS             15826..18504
                     /gene="nuoL"
                     /locus_tag="VGB76_01230"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY0097548.1"
                     /translation="MHRLIWLIPLLPLAGAAINGLLGRRLRFSETVVSVIAVGSVALA
                     FLLGVAAVYSYGFGGHAQWPQPYVTSQDGAFVYRWIPGGATELTQGTGHGVYREISAA
                     TRAKTEERAREITRETGTPPSAVLMGGEPETRGLGALLDVEWSYQLDPLSSIFILVIT
                     GVGLCIFIFATGYMHGDAGFYRFFAYLGLFMFMMLILVMGSNFLMMFVGWEGVGLCSY
                     LLIGYYFDRKEAGDASRKAFITNRIGDFGFALAVFGIIATFGSTQYTQVMSAASGYPV
                     EPLGQFGLMSWLALGLFVGACGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGLY
                     MLTRTNVIFQHSQTMMLVVAIVGALTALFAATIGITQNDIKKVLAYSTVSQLGFMFLA
                     CGVGAFAIGIFHVMTHAFFKALMFLGAGSVIHGMHHEQDMRRMGGLRKYMPKTYLTFL
                     AGWLAICGIIPFSGFWSKDEILWNAASTTLIPYGWLLWLVGTFTAALTAFYMTRLVAM
                     TFWGRERFLDVDAGGQADEAHAHGYDDKGKTHDAHIDSAGDRPRHEPGEYVGEPHAGA
                     AHAHTDAHGAPVAVHHDAHGHGTHIPHESPPAMWIPLAALAVLAVIGGFVGISPAFTG
                     GAHVGGRMNIVNWLEPVIWNPSTRQFNAPHGAGGGEHASADTHGEPDAIAQNHGAPAT
                     TQAAPAPHSDTAQSSHAATGADASHSPYGNDAGFNLAHAVEGVTHSHLLTEWVFIIIS
                     LIAAGIGMGLGYLFYVKNPRLPEVWATRLGALYRASYNKYWVDELYGLLFTRRTMDAA
                     RGIYAVDSKGIDGAVNGSAWLTRRISRYTGNFDRTVVDGAVNGIAVFIGRLMSPLLRA
                     AQTGLTANYALVMVLGLVVAVVFFFSGDILGLVRGR"
     gene            18560..>19027
                     /locus_tag="VGB76_01235"
     CDS             18560..>19027
                     /locus_tag="VGB76_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097549.1"
                     /translation="MKIPYLLSIITWLPLVGALIILFVFKKDNANAIKKFATGWFGLV
                     FLVSLALVGYDRAAGGMQFIEDLQWIPIIGARYQMGVDGVAVLLIVLTTLLGVIAALS
                     SWEYIGKREQEYYVLLLLLQAAVVGVFASMDLFLFYLFFEVSLVPMYFLIGIWG"
BASE COUNT         3507 a         5726 c         5860 g         3934 t
ORIGIN      
        1 gcctttgaac tggaactcat cggtgcggaa gaaggcggag gagacgccga cgcggtcgca
       61 gctctggttg cctgcggggc agttgttgag ggcgttgacc caggcggaga cgccggtggg
      121 ttcgccctga cggttgagga agtcgacata gtgttgttcg acgaagaact gcgcgctgtc
      181 tacagggttc ggcgcgttcg ggttggtgtc gttatcgacg ataacgacga cggcggagaa
      241 gattgttcca atggtgaggc cgctggacgg attgctcaat gtgacattga accgctcgtc
      301 gccgtcgagg tacacgtcgt cggtgatgaa gatgcggaag gtcttgctgg tttcacctgc
      361 ggcgaagcgc agcgtgccga gcgtggtggt gtagtcgcag cgttcggagg cgaaaccgtt
      421 cttaacattg cagccgacgg taacgctatc ggcttcggtt tcataatcga cggtggccgg
      481 ggcagcggtg tcgcccgtgc ggaccactgt gatgagaacg ctgcgcgcgt tttcgtcaac
      541 cacgtacgcg ctcgcgctga gttccaccgt atgcggcgtc ggcgtgggtg acggcgtagg
      601 tgtcggcgtt ggcgtcgggg ttggagtggg cgtaggtgtg ggcgtcgggg tcgggtcttc
      661 cgcggctttg tagcgtgcga gggcaaagtc cctgctgctg cccgtgtccg tgtatccgcc
      721 cgcgacgatc ttgccgtctg cttggataag gacggcggcg gcttcgtttt caatgccgaa
      781 gagatcggtt gtgacctgac cgcccgcgcc gaaacttgaa tcgagcaggc cgtccgtgtt
      841 gtaacgcgcg agggcgaaga acgagaacgc agcagcacgc gcagttggct gcgcttgacc
      901 gttaaccaat gcggcgggct tcgcctgccg cccggacttc gcggcgtccg agggcgtctt
      961 gtaaatctct acgtcatcgg ttgcgcgtgg ctgcacgcgc ccctccgccg ttgtgacgac
     1021 ggagagcggg aaatagaggc cgactgcgac gagcttgccg tcggcttgca gagcgaggtc
     1081 gttcgccgag tcgaaaccgc cgaagtcggt cgtgactttg ccacccgtgc cgaatgaatc
     1141 accgggcgtc gagtctgtgc ccgtgccgct gtcgagcgag ccgtccgtgt tgtaacgcgc
     1201 gagggcgaaa tcgctgttgc cgccggagat ggtagcccct cccgccgcga ccaatttacc
     1261 gtctgcctgc ctgaccagag cgttgatgcc gtcgccgccc gcggagaagt ctgtgaggac
     1321 tttgccgccc gtgccgaagc ctgtggtgtc gagcgagccg tcggcgttgt agcgcgcgag
     1381 cgcgaagtcg ccggacgagg agcctgcggc gacgatcttt gcgtcgggtt gaatcacgac
     1441 cgcgtgggct gcatcgctcc gctcgttgaa atcggttgtg actttgccgc ccgtgccgaa
     1501 catgtcggcg ggtgtcgagt cgcccgctgc gccgctgtcg agcgagccgt tggcgttgag
     1561 gcgtgcgagt ttgaagttta agaaatcccc gccgacgagg acgatcttgc cgtcgggctg
     1621 caaggccacg tctgatgcgc cgtcgtgtcc cgttccgaaa tcaaccgtga cgcggccacc
     1681 cgtgccgaag tccgtgtcga gcatgccgtt ggcgttgtag cgcgccacgc cgaaagaggt
     1741 gtcggcagag ggcacgatgg agaattgcgg tccggtctgc ccggccacga cgatcttgcc
     1801 gtcgggttgc agcactacgg cgcgtgcaaa atcgtcgccg ttgcctgtga agtcggttga
     1861 aaccaagccg ccggagccga aggtggtgtc gagcgagccg tccgggttgt agcgcgcgag
     1921 caggaagttg aagtcctgcg catcatcaat gatgactcgc gtgttcatgc gcccgaccgc
     1981 gacaatcttc ccgtccggtt ggagcgccat gtcttcgatt gtttcctcgt tgagggggaa
     2041 gtctgtgaac accttcccgt ctgtgtcgaa ggagttgtcg agcgagccgt ttgtgttgta
     2101 gcgaacgacg ccgaaatcga gttggccgct cgtgttgtcg ccgcctgcga cgacgatgcg
     2161 cccgtccgtg tagagagcca cgccccgccc gccgagattg aagcgaccgc ctgcaacgtc
     2221 ggtcaaaact ttgccgtcgg tgtcaaagga gttgtcgagc gagccgttcg tgttgtagcg
     2281 cgcgaccgcg aagaaggtgc cggtcgggat actgcccgct ctaccggcgg cgacgatgcg
     2341 cccgtccggc tgaatcaaga catcccttgc gccgtcaacg aggtcgaagt cggtcgtgac
     2401 tttgccgtcg ccatcgaaag aggtatcgag cgagccgtcc gtgttgtagc gcgcgagcgc
     2461 gaagttgtcg tcggcggcga cgctcgtggc aatttcaccg gccacgacga tgcgcccgtc
     2521 cgtctgaatc gcaaccgcgt tggcctgttc aaaagagccg ccgaagtcgg tcgtgactag
     2581 tccgtccaga tcgaaagtgt tatcggggct gccgttggtg ttgacgcgcg cgacggcaaa
     2641 gacggaactg ccgaggccga ccgccacgat gcgcccgtcg gcctgcacgg cgacgcccaa
     2701 cgcttcttca aaggtgacgc ccgcgaaatc aatcgtgagt ctgccgtcgg tgtcgaaggt
     2761 ggtgtcgagc gagccgttcg tgttgtagcg cgcgagggcg aagacgctct cgaaagtcgt
     2821 cccgttcctg ctcgatcccg cgacgacgag tttgccgtcg ggttggagcg caagggcgtt
     2881 ggcagttgcg ctcgcgcccg taaacggcgt cacgaccagc ccgtccgaat caaaactcgt
     2941 gtcgagcgag ccgtcagggt tatagcgtgc gagggcgaag gcggagttga aatcgtcttc
     3001 gggggagttg gccgacccgg caaggacgag cttgccgtcg ggctggataa cgagtgcgga
     3061 ggcgaaaccg ccctccccga tagggaagtc tgtgttgacg cgaccgcccg tgccgaaagt
     3121 ctggtcgagc gagccatcca tgttgtagcg cgcgacggcg aaatcggtgc cggagccacc
     3181 ggccaccacg actttgccgt ctgcctgcac ggcgacatcc tcggcagcgt cgaggccgtt
     3241 agggaacggc gtgacgacct tgcccgcgtt gccgaacgtc gtgtcgagat cgcccggagg
     3301 ggctaagagc gaccgcgcct tgtcggcctg cgcggaggca gctttaagct ctgcggtcga
     3361 gaggggcgcg gcggcgagag agcggagcca gaagaaggga gcggttaaga gaacgaatgt
     3421 cgaaagcagt atggctatga aggccacggc gcgtcgtgag acaggaattg agcggggagc
     3481 ggggaattgc ttcataaagt ctcctgtaaa tttggaagcg atggctggtc gaacagcatt
     3541 ctctttgcgg ctctatacgg cgtgcgacga agatgcagcc ctgtacccgg tgataagtgc
     3601 ggtctgaaaa acggtaggcc ggttttctac tcctaaacaa aatttaagtc aagaatttta
     3661 atgacgccgc cgacgcgcgt gagcgtcgag ccatgtcccg gcgtgcgcgc cggaggcggg
     3721 cgcacaaacg cattgacaag ccgtaccccc gcgcttatat ttcgcttgtt cactgtagac
     3781 ctgaagcctg aactcgcgcc ttcccgtcaa aaaccggaaa ccatgctgag cttgatactt
     3841 ctccaggcgg caaacgcaat cacagatgca acggcggcaa cgaccgcgac cgctgctgcg
     3901 cccgtcgcac aggtgggcag cccggtcacg gcctactggc cggtgatgat cttcttcgtc
     3961 gtggccgtcg tcttcccggt gctgccaatc gtgctggggc gtctggtgcg cccgagcatc
     4021 tggcaaaaag ataagatgcg cccttatgaa tgcggcattg atccgacgac ggaggcgcac
     4081 gaccgtttca ccgtgcgcta ctacatcgtg gcgatgctct ttctggtctt cgacgtggag
     4141 acgatcttcc tgctgccgtg ggcgatcatc ttcatgggcg acgatttctc cttcaccaaa
     4201 ttcgccctcg tcgagatgct cgtctttatc ggcatcctcg tcctcggtta ctactacgcg
     4261 tggcgcaaag gcgcgctcga atgggcttga actacggtga cgggtgacaa gtgacaggtg
     4321 acaggttaaa gcagaagaaa aactctcttt aaacttgtct tgttccaact cgtcacttgt
     4381 cactcgtcac ttgtcactta aacatatggc agagcatccg gaaggaatcg tactgacgac
     4441 ggttgacgcg atcttcaatt cgttgcggcg caacgtggct gcgcccgtga tgaatgtgaa
     4501 gcccgtgcgc aacactgtga actgggcgcg caagtctgcg ctgtggccga tgacgttcgg
     4561 gctggcctgc tgcgccatcg aaatgatcgc gacgggcgcg ggtcgcttcg atatagatcg
     4621 cttcggcgcg ggcgtctttc gtcccagccc acgccagagc gacttgatta tcgtcgcggg
     4681 cacggtgacg ttgaagatgg ggccggtcgt gcgccgcgtc tatgatcaga tgccggagcc
     4741 gaaatgggtc atcgcgatgg gcgcgtgcgc ttcgaccggg gggccgttcg attcctactc
     4801 cacgatgcag ggcgtagacc gcaccatccc cgtagacgtg tacattcccg gttgtcctcc
     4861 gcgccccgaa gccctgctct acggcctcat gcgcctacag gacatcatca tgaaagaagc
     4921 cccctccgcc tacatcttcg actccgacgg cacagtccat cgccaacgcc aactcgaagg
     4981 cgacggcgaa gaagatgcga gactggacgc cgaacgcgag gacgacgaag tctccaccgt
     5041 cgccggttag caaacacacg cacgtctcgc gccgcgcacg ccgcacacgc aatccgagca
     5101 gtatctcaag ccgcctcttt accggaaggc ggcttttgct tgtcgaattt tgcttgtcga
     5161 agttgcccgc gccgctcgtc aggcttttgc gtcgggtgat aagtcgccga gatggcgcgt
     5221 gtcgttgagc gcggagatgc gcaggaagcc gtcgtcgcgg agagtgaagc gggagatgcc
     5281 gcagttggct gtggcgagcc agacggggcg cagggtgggg gcgtcgagtg cgcccaagag
     5341 gtgcagggcg aggatgcaga tggtgccggt gtgagagaag actgcgatag tgccccctcg
     5401 attggcattg atggcgcggt cgagcgcgtg ggcggcgcgg tcgagcagtt ggcgatagct
     5461 ctcgccgccg gggagcacgt gctcgaagtc tctgcgcagc agcgcggcat actcttcggg
     5521 aaacttttgc gccgcttcct caaacgtcaa gccttccatc cgtccgatgc tgcgttcacg
     5581 aaaggcgtcg ctctgcctga tctccaaatt gagcgcggcg gcgagcggag tggccgtctc
     5641 gacggcgcgc agcaggtcgc tcgaatagat cgccgtgacg ccttccgact ccagcgcacg
     5701 cgccgtcgcc tccgcctgcc tgtgaccgag cgcggataag ggcgtcggcg tgtgcccgcc
     5761 gaagcgacgc gcggcgttgc cctcggattg accgtgacga atcagcagaa cgcgcgtgct
     5821 ctccataaga agacagtgac aagtgacgag tgacaggcag gaacagagca aacctgctgc
     5881 cattaacatg ctgctaaaaa cgacaagtga caagtggcga gcggggaagt ttaaagagca
     5941 gagcgctgtt ttaacttgtc acctgtcact cgtcactcgt cactctcttc tacgccgcga
     6001 cggtttcggc cttcagcttt tcggcttgat agtgtgtgac gagggcgttg atgatctcgt
     6061 cgagttggcc ttccatgacg aggttgagtt ggtgcacggt gaggccgatg cgatggtcgg
     6121 agacgcggtt ctctttgaag ttgtaggtgc ggattttttc ggagcggtcg cccgaaccga
     6181 cctgcgagcg gcgttcgctg gcgagcagtt cgtgctgctt ttgctcttcc tgctcttgca
     6241 gccgcgcacg cagcacgcgc atggctttct cgcggttctt gatctgcgat ttctcatcct
     6301 gcatggagac gacggtgttc gtcggcaggt gcgtgatgcg gaccgccgag taggtcgtgt
     6361 tgacggattg gccgccggga ccagatgagc agaaagtgtc gatgcgcaga tctttcgcat
     6421 cgatcttcac gtcaacttct tcggcttcgg ggaggacggc gaccgtgatc gcgctcgtgt
     6481 ggatgcgccc gctcgtctcc gtctgcggca cgcgctggac gcgatgcacg cccgattcgt
     6541 gtttcaactt cgaatagact ttgtcgcctt cgatgagcgc ggtggcctct ttgtagccgc
     6601 cgattcctga ctctgaggtg ttgagaatct ccatgcgcca gccctgacgc tctgcgtagc
     6661 gaccgtacat gcgcagcatc tcggcggcaa agagcgaagc ttcatcgccg cccgtgcccg
     6721 cgcgaatttc gaggatgacg ttcttctcgt cgttcggatc ggtcggcagc aacaggaggc
     6781 gcaggtcttc ttcgcaccgc tccaactgca cttgcagagt ctcgacttcg acgcgggcga
     6841 gttcgcgcat ctcttcgtcg tccgcgttca tgtcgaacat ctcacgcgcg cccgcgagtt
     6901 cttccttaat cgtcttccac tcgcgatacc gcttcaccat atcgccgagg ctgcgatgct
     6961 gcttggcggc tttggcatag gccgcctgat ccgccagcag ttccggcgaa taaagttctt
     7021 ccgacaacgc ttcgtagcgt gcttcgatct gttctaattt ctcaagcata aaatagctct
     7081 cagtaatcag ccgtcagctt tcaacttccg gccttgtcat tcgtaaataa acaccctgct
     7141 tgacgatcaa tttgttcaac tgctggcgca aaaaaagctg acagccgaaa gtcggcggct
     7201 ctactctaag agagcggcgt catggtggct tcctgcggtc gcggttcgag tttgagcgac
     7261 tctttgagcg cgtagagggc gacttcgagt tgcttgtcgt cgggttcttt cgtcgtaatg
     7321 ttttgcagcc agatgccggg cagcgtgatc attttgaaga tgaggctgcc ttcgcgtctg
     7381 gccgagagcc ggatgatctc ataagagagg ccagcgataa cgggcatcag cgcgagacgc
     7441 gtgagcatgt tcaaggcgag cgaatcgaac ttaacgatgg agaacaggac gatggagacg
     7501 agcatcacga ccatcaggaa actcgtgccg caacgcgggt gctggcgcgg ctgcgtgcgc
     7561 gcgtgctcaa ccgtgagcgg caagcccgcc tcccacgtga agaccgtctt gtgttccgcg
     7621 ccatgatatt caaagacgcg ccggatgtcc ttcaagagcg agaacgaaaa gatcatgatc
     7681 aggaagaacg acatgcgaat gacgccgtca accagattga aggccaccga agggcgcacc
     7741 ggatggagat aaccgcgcac gccgccccac gcacgcgcgt accatgcctg ctcgctcgtc
     7801 gccgtttgat tcgtcgcgga cgcgcccgtc gttgtgttgg cgacgctttg agtttgcgcc
     7861 attgcggcgt tcgcgttcat cccggcgggc acagcccaac cggcggcgat gaagagcgcg
     7921 ttggttaaga cgagcggcgc aacgataaag agcatgacgt tgaagaagag tgcgaagacg
     7981 atggagccga tgacggaggc gttggcgccg cgcttcatct catcttcagc gcgccgctcg
     8041 gtcgggatgg gaatgagtcc gggcacgccg ccagtgattc ccgcgaagtc gccttcgccc
     8101 tcgacgaccg ccggagtgag tgccaccgac gccacctcct gctcctgctc cgcatctgcg
     8161 aaggcttcgc tcgcggaata gttgagagcc ttgatgccga gcgccatcga ttgcacgagc
     8221 accgctgcgc cgcgtaagac cggcagccgc aggaaacgat atttatcgct ccacttcggc
     8281 agagttgcgc ccgtgaagac gatctcgccg tccgcgcgcc tgacggcgac cgcataagcg
     8341 ttcggcgttc gcatcatcac gccttcgacg accgcttggc cgccgacgat taggtctttt
     8401 tcgtttgcac tcactttgtt tttgctctct gaaaatttac agggggggtg agataacaac
     8461 gccgacgcta tcaggttcgg aggtcgctgt gtgcatcaat ggatggatgc gcgggcacga
     8521 ccgccgcgct tgcaagcgtc ggcgtcaaat tttgggtcgc taatactggc cggagatagt
     8581 ctgcttcatt gacacagagt gcccgtcgtg cgagaaaccg gaacgggcgg gagcctcgat
     8641 gcgcctctat tagctggcgg cttgttcgct cgttgccgca ggggcggttt cggtcttctt
     8701 gccgtaacgc ttgttgaagc ggtcgacgcg tccggcggtg tccacgagtt tctgcttgcc
     8761 cgtgaagaac gggtggcagg ccgagcagat ttcgacgtgg agatcgtcct tacgggttga
     8821 acgcgtggtg aaattctcgc cgcaggcgca gacgactttg atttcgttgt atttgggatg
     8881 aattccgtct ttcatgtttt ccttcctcac gacagatgcg aatttgggtc atctgtcaat
     8941 ccggtatgat actttttcgg tattcttatc gtcaagttag atggggattt atggcttgcg
     9001 gtggcctctt gggaacggcc tgataagttt attgacaatg ccgtaaacag cgtgttaaaa
     9061 attctgctcg tcaccgcctt tttgcttctt tgtcagaaca caagtcgaaa gtttttgagc
     9121 attaaacccg aacggcgcac actccgcccg tttttccgtc gaagtctaac ttttcgctcc
     9181 gatgattgac gagactgaca aaacgcagtc cgacgattct gccaaccgcg cgacggacga
     9241 cgcgtccgcg cctttacgcc ccgacgacag atcgcccagc cccgacggag aaatttcacg
     9301 agccggaagt ttgcccgcgc cgccgcacgc cgacgcgccg cccgcgcccg aaagtcaggc
     9361 cgcgccgcag gcgaaagctc cttcgggaca gtcgcgcgac accgtgatgg gcgcgtcaac
     9421 cggagacgcg tcaaacgccg cgcccgacgc cgccgacgcg ccgcccgccg acgaaactcc
     9481 cgaacagaaa cgcgcgcgca ttatcgccga ggcgaaagcg aaagccgccg ccaagatggc
     9541 cgccgcgaaa ggcaacgcca ccacggcggg cggcgaaacc gcagccccga aggccaagcc
     9601 gccgggcgtc gcgccgtctg ccacctccga cgaggagaag gccgcgcgga tcgccgaggc
     9661 gcgcgccaaa gccgccgcgt tcaaacaagc ggcgggcgag tctgccgggg cgaacaagac
     9721 accgcccgcg ccgtttgccg ctgccggtgc gcctgccgcc cctgctgctt ctgctgcggg
     9781 tgcgccgaaa gctcccgtca agaagaaaga ggaaggggcg aagcctgcgg acgcctcgca
     9841 gcacccgctc gtcaaacgcc tgcgggaagg tctggacggc gtggtcatcg aagcgacgga
     9901 gtttctcggc cagttgtccg tgcgcgtcgc gcccgcgcgt atcgtggaag tctgcgagtt
     9961 tctcaaacgc gatgcggagt cgccgttcga atatctctcc gatctcacct gcgtccattt
    10021 tcccgaaaac gcgggcgcgc ctttcgaggt cgtctataac ctgtattcga tcagcgcgaa
    10081 tgtgcgcgtg cgtttgaagg cagagacgac ggaggccgcc gggatcgaga gcgtgacggg
    10141 cgtgtggccg gcggcaaact ggttggagcg agaggtctat gatctcttcg gcgtgcgctt
    10201 ccacaatcac ccggacttgc gccgcatcct gcttccgccc gactgggaag gccaccccct
    10261 gcgcaaagat tacccgctcg aaccggtgga gaataactgg acggccaagc acctgccgga
    10321 gttctccgac gtgcagcgcg aacaactcga acaacgccgt gcctacggcc tcgaagccct
    10381 gtccacgccg gacgaaaggc gcgtccgcga aatcttccgc gcgggcaaag aagtgatgcc
    10441 gctcgataga aaatgatgaa cgatgaacgc ggaacgatga atgtaagaga gcactctctt
    10501 catcggacag tttagccttc atcgttccgc gttcatcgtt ccgcgttccg cgttcatcgt
    10561 ttaactttat gcatcaggta gaagtcgcca ccacgcaaga aaccctgttg gacagcccgg
    10621 aaatggtgct gaacatgggg ccgcagcatc cttcgacgca cggcgtcctg cgcgtcatcc
    10681 tgaaactcga cggcgagacg gtcatcgatc tcgattgcga catcggctac ctgcatcgcg
    10741 gcatggagaa gatcgccgag catcgcctct acccgatgat cgcgccctat tgggacaggc
    10801 tcgactacat cgccgccgtc tcgaacggcc tgctctgggt ggagacggtc gaacagatgc
    10861 tcggactcga agtgccgccg cgcgcgcact acatccgcac cgtcctgacc gaactcaacc
    10921 gcatctcttc acatcttctc tggctcgcca cgcacggact cgacatcggt tccttcaccg
    10981 tcctgctctg ggctttgcgc gaacgtgaag agattctaaa gattttcgaa cgccagttcg
    11041 gcgcgcgtct cacctgtcac gcgtggcgca tcggcggcat gccgaacgat acttacgacg
    11101 gcttcacgga agacgtgacg cagttcgtag acaactttcc gcaccgcctc gaagagtacg
    11161 agacgctcct gtcggagaac cgcatctggc tcggtcgcac ggtgggcatc ggcgtcatct
    11221 cggcggagga ggccatcgcc atcggcctct cgggtccggc gttgcgcggt tcgggagtcg
    11281 cttgggacat acgcaagtcg cgcccgtatg ccgcctacgc cgacatggaa ttcgatatcc
    11341 ccgtcggcga aaacggcgac acctacgacc gttacctcgt caggctcgaa gagatgcgac
    11401 aatcgcggcg catcgtgcaa caggcgctcg cgaagatgcc ggacggcccc gtcaacgcga
    11461 aggtgccgaa gatcatcaag ccgcccgtgg gcgactatta tcattcgatt gaaggaccga
    11521 agggcgagat cggctgctac ctcgtctcgg acggttcttc cagaccggcg cgcgtgcgcg
    11581 tgcgcccgcc gtgcctgatc aacttgcagg cactccggca gatgtgcctc ggccatctcg
    11641 tcgccgatgt cgtcgccatc atcggcaccc tcgacatcgt gctgggagaa attgatagat
    11701 aaagcagtga caggtgacag gtgacaggtg acaggttaaa acaacagaac ttgtctttaa
    11761 acttgtctcg attcctgctc gtcacttgtc actcgtcact ggcttatgag agttgtgaga
    11821 ctcgctgtgc aggagatgac gcgcgagcgt ttcgcgccgt tcgggatttt gctcgatagt
    11881 cgcgggtcgg tggagattga cctggggatg ggcgcgccga gtttgacggg cgcgacgagc
    11941 gagcggcgcg cgttcgagtt tcagtttatg gcgcggcaca agcgtacgat gcaggtcttt
    12001 tcgcctctgg cctcttcgcg ttcaatcatc tgtgtcgcgc cgccgaccga gagagacgcg
    12061 cccgcgttgg atgaggtcgt cgcttttctg gtcaacggga aattgcctta cgcctatcac
    12121 aagggcacct ggcacacgcc gcccttcgcc gtgggcgagt gggcttccta cctcgtcgtt
    12181 gaccgcgccg gaacgctcga tagcgattac gagttggttg atttgaaagc cgtccacgat
    12241 tgtactttcg agatcgatgt ctgagacgcc tctgctttta actggccgtg aagtgaaaga
    12301 gaggtgcaaa ctatgagcac agaccgcaca accgaaattc tcaactacct gagcgcgatc
    12361 agtcgcgatg tcggcgaact gcgcacggag gtacacagtc tccgcgatga gatgagcgcg
    12421 cgtttctcgg aagtgaatac gcggatggat cgtatcgaaa ccgaggtcac acgcctcggg
    12481 cgcgagcaac gtctgctcaa ccgtatcgga tcggtgatgt tggagacgcg ggcggatatt
    12541 gaggaattac aagaacgcgt agatgtgctc gaaggcaaat agcggagcgt ttgaatcaac
    12601 ttatggacat gctcgcatta ttcgcgccgc cggatttaat caacggcctc ctgctcaaac
    12661 tcgggcttgg gcgggagacg atcaactacg tcgtctggcc gttcatacag atcggcgcgg
    12721 tggctaccgt gctcgccggt tgggcggcct acgcctcgta tctcgaacgc aagatttcgg
    12781 ccttcatgca ggcgcgtctg ggtccgatgc gcgtcggtcc gtggggactc ttgcagccca
    12841 tcgccgacgc catcaaactg ctcacgaaag aagacctcaa gcccgaccgc gccgacacct
    12901 atatcttctt ctacgcgccg ttcatctcgg tggtggcagc cttcgtcgtc ttcgtcgtga
    12961 tccctttcgc gcccgattgg ggcgtcatta cagacctcaa catcggcctg cttttcgtgc
    13021 tctcagtctc ctctgtcggc gtgctcgctc tgatcctcgc gggctggtct tccaactcga
    13081 aatacgctct gctcggcggc ctgcgctcca gcgcgcagat ggtttcttac gaagtggcaa
    13141 tgggtatggc gctcatcggc gcgctgatgt tctcgcgcac gctctctctg tcgggcatcg
    13201 tctcggcgca ggggctggat tccatctggt acatcttcta tcaaccgctc ggcttcatcg
    13261 tctttctgat cagcggcatc gcggagaaca accgcgctcc gttcgatctg ccggaagccg
    13321 aatcggaact cgtggcgggt tttcacacgg agtattcggg gatgcgctgg tcgctcttct
    13381 tcatggccga gtacgcggcg atgatcgtgg tgtcggcgat tgcgacggcg gtgtttctag
    13441 gcggctggta tttcccgttc gtgtggcgtc tgtcggccag ttacccgcaa ctctacgttg
    13501 ccgtcagtct tctggtcttc atcttgaaga cggcgtttct gctctacgtc tatttctggc
    13561 tgcgctggac gttcccgcgt tatcgctacg accaactgat ggatttgggt tggaagtgga
    13621 tgattcccgc cgcgctcctc aatattacgc tcacggcaat cgcgatcttc acggtgcagg
    13681 cgctcgacgg gtggcgcggt atggacacgg tcgagtccat cgcgcgcggc gtgaacctga
    13741 cgcggatagg caaagcgatc atgatcgcgt tcggcgcgct cggcgtgttc atcgcggcag
    13801 gaatgctctc ttacatcaat cacggcacgc gcgacttcaa cttgaagaag cagcggcgcg
    13861 gtattcaact cgtcaacccg ccccaaggcc aaacggcggt cgccagcaca acgacggcgg
    13921 cggtcgcgtc ggaaggataa agtaaggata aagttatgcc ggaagtttat gtcccagagg
    13981 cgacctggaa agagaagtta aaacgtttct tcctgatcga tttgattcag ggcttgaagc
    14041 tgacgctgaa attcaacgtc ggcgcgttaa cggacaagga ttcggtggcg ggcaagggga
    14101 tttacacgga gcagtatccg aaggtgcgcg cggaagtcgc gccgcgtttt cacggcgcgc
    14161 cgcgtctcaa tatggacccg gagacttacg agacgctctg catcgcctgc aacctctgtg
    14221 cgctcgcctg tcccgaagat tgcatcgacg tgatcccgat ggacatcgag atcgtcacgg
    14281 cgggcaaggc gcgcaagaag aaggtgctcg acgaattcat cttcgacacc tcgcgctgca
    14341 tgttctgcgc gctctgccag gaagcgtgcc cgaccgcctg cctcgaactg acgcaggaat
    14401 tcgaactcgc cacctactcg cgcgcaggct tcgtctggaa tcgcgaaatg ctcgaacagg
    14461 gacgcgagac ggttaaatac gaaaagtgat gagtaatgag taatgagtga tgagccgaaa
    14521 gctctcacca cttactcacc actcatcact cattactcat cactcatcac tatgacctta
    14581 tccggttggg aagcggtctt cttctgggtc ttcgcgattg ccgcgctcgt ggcggcgttg
    14641 ttgaccatcc tcgcgcgcaa tgccgtccac tcggctctgt tcctgatctc gacgctcatt
    14701 tccgtggccg cgctctttat cctgctcggc gcggagttca tcgcgggcgt gcagattctc
    14761 gtctacgtcg gcggcgtgat ggtgctcttt ctgttcgtca tcatgctcgt caacgtgggc
    14821 gcggaggagc gcggcggcga gatgttgttc aatcgtcccg cgcaggtggc gacgagcgcg
    14881 atcttcctcg tgctgctctc gctcggcttc atctacgcca tccgtcaggg ttacgaagga
    14941 ctactcatcc gcgacaatct cgacgcgccc gcacagcgtg cgcaactcgc cgaacgacgc
    15001 aacgcggaac agcaaaattt gcggctggcg gcaaccggca cgtcgcgcat ttcgaccgac
    15061 acgcaactcg tcggcgcgag cctctatcgc tttgccgcgc tgccctttga aatcgcgagc
    15121 gtgcttctct tagtcgccat tatcggctcc gtcatgctcg cgcggacggc caaacaggaa
    15181 gaggccaccg atgatattga gacaaaggat gcgggataag ggatgcggga tgaatcagaa
    15241 aacattcaag ccaactggcg gccactgttt ttaattcatc ccccgtccct cgtcctccat
    15301 cccctatctt tcaaccgctt atgaacaact ttatcgcatc cgcgctcgca ttcgttttgc
    15361 aggaggctgt gccgggtcgc tggcagatga tcaagcaggg cgtgcttgag ccagacctgt
    15421 cgaagtttct cgtgatcgcg gcgttgctct ttgtcatcgg cgtggcgggc gtgttgacgc
    15481 gtcgcaacat catcgtcatc ttcatgtcca tcgagttgat cctgaacgcg gccaatctca
    15541 acttcatcgc cttctcgcgc tacttgcagg acacgggcaa gatgaacgcc gtcgccggac
    15601 aggtctttac gatctttatc atcgtcgtcg ccgctgccga agccgccatc ggcctcggcc
    15661 tcgtcatcgc gctctaccgg aacaaagaga cgatctttat tgacaagatt gatctgctca
    15721 aatggtaaaa aaacagtgac aagtgaagag tgacaagtga cgagttggaa agacaagttg
    15781 ttttaacctg tcacctgtca cctgtcactt gtcactcttg aatatatgca cagactcatc
    15841 tggctcattc cgcttctgcc gctcgccgga gccgccatca acgggctgct cggacgccgc
    15901 ctgcgcttct cggaaacagt cgtcagcgtg attgcggtgg ggtcggtcgc gctcgcgttt
    15961 ctgctcggcg tggcggcggt ctattcgtat ggcttcggcg gtcatgcaca gtggccgcag
    16021 ccttacgtga cgagccagga cggcgcgttc gtctacaggt ggattccggg cggcgcaacg
    16081 gaactcacgc agggcaccgg tcacggcgta taccgggaaa tatccgcagc cacgcgcgcg
    16141 aagactgaag agcgggcgcg cgaaattaca cgcgaaaccg gcacgccgcc gagcgcagtt
    16201 ctcatgggcg gcgagccgga gacgaggggc ttgggcgcgc tgctcgacgt ggagtggagt
    16261 tatcaactcg atccgctctc gtcaattttc atcctcgtca tcacgggcgt cggcctgtgc
    16321 atcttcatct ttgcgacggg ctacatgcac ggcgatgcgg gcttctatcg cttcttcgcc
    16381 tatctcggcc tcttcatgtt catgatgctc atcctcgtga tgggatcaaa cttcctgatg
    16441 atgttcgtcg ggtgggaggg cgtcggcctc tgctcctacc tgctcatcgg ttactacttc
    16501 gaccgcaagg aggcgggcga cgcctcccgc aaggctttca tcacgaaccg catcggcgac
    16561 ttcggtttcg cgctcgccgt cttcggcatc atcgccactt tcggttccac gcagtatacg
    16621 caggtgatgt ctgcggcttc cggctacccg gtcgaaccgc tggggcagtt cggcctcatg
    16681 tcctggctcg cgctcggcct cttcgtcggc gcgtgcggca agtctgcgca gatacctcta
    16741 tacgtgtggc tgcccgacgc gatggcagga cccacgcccg tctccgcgct catccacgcc
    16801 gcgacgatgg tgaccgccgg cctctacatg ttgacgcgca ccaacgtcat cttccagcac
    16861 tcgcagacga tgatgctcgt cgtcgccatc gtcggcgcgc tgacagccct gtttgccgcg
    16921 accatcggca tcacgcaaaa cgacatcaag aaagtcctcg cctattccac cgtctcgcaa
    16981 ctaggcttca tgttcctcgc ctgcggcgtc ggcgcgttcg ccatcggcat cttccacgtc
    17041 atgacgcacg ccttcttcaa agccctcatg ttcctcggcg caggctccgt cattcacggc
    17101 atgcaccacg agcaggacat gcgacgcatg ggcgggcttc ggaagtacat gccgaagact
    17161 tacctgacgt ttctggcggg ctggctcgcc atctgcggca tcattccgtt ttcgggtttc
    17221 tggtcgaagg acgaaattct gtggaacgcg gcttcgacca cgctcattcc ttacggctgg
    17281 ttactctggc tcgtcggcac tttcaccgcc gccctgaccg ccttctacat gacgcgcctc
    17341 gtggccatga ctttctgggg gcgcgagcgt ttcctcgacg tggacgccgg agggcaggcc
    17401 gacgaagcgc acgcgcacgg ctacgacgac aagggcaaaa cgcacgacgc gcacatcgac
    17461 tcggcgggcg accgcccgcg ccacgaaccc ggcgagtatg tcggcgaacc gcacgccgga
    17521 gcagcgcacg cgcacacaga cgcgcacggc gcacccgtcg ccgtccatca cgacgcacac
    17581 gggcacggca cgcacatccc gcacgagtcg cctcccgcca tgtggattcc gctcgccgcg
    17641 ctcgcagtgc tcgcagtcat cggcggcttc gtcggcatca gtcccgcgtt cacaggcggc
    17701 gcacacgtcg gcgggcgcat gaacatcgtc aactggcttg agccggtcat ctggaatcct
    17761 tcgacccggc agttcaacgc gccgcacggc gcagggggcg gggaacatgc gtctgcggac
    17821 acgcacggcg agccagacgc aatcgcgcag aatcacggcg cgcccgcgac gacgcaagcc
    17881 gcgcccgccc cgcactcaga caccgcgcaa tcgtcgcacg cggcgaccgg agcggacgca
    17941 agccattcgc cctacggcaa tgacgcgggc ttcaacctcg cgcacgcggt tgaaggcgtg
    18001 acccatagcc acctcttaac cgaatgggtc ttcatcatca tctcgctcat cgccgcgggc
    18061 atcggcatgg gactcggcta tctgttctac gtgaagaatc cgcgcctgcc cgaagtgtgg
    18121 gcgacgcgtc tcggcgcgct ctatcgcgcg agctacaaca aatactgggt ggacgaactc
    18181 tacggcctcc tcttcacgcg gcgcacgatg gatgcggcgc gcggcatcta cgcggttgac
    18241 tcgaagggaa ttgacggcgc ggtcaacggc tcagcgtggc tcacgcggcg catcagccgt
    18301 tacacaggca acttcgaccg caccgtggtt gacggcgcgg tcaacggcat tgccgtcttt
    18361 atcgggcgtc tcatgtcgcc gctcctgcgc gcggcgcaaa ccgggctgac cgccaactac
    18421 gcgctcgtca tggtgctcgg cctcgtcgtg gcggtcgtct tctttttcag cggcgacatc
    18481 ttgggtttag tgcgcggaag gtagagcgtc cacctgtcgt ccgtgcgccg tcgtccgttc
    18541 tctctgactg ttgaccaata tgaaaatccc ttacttactc tccatcatca cgtggctgcc
    18601 gctcgtcggc gcgctcatta tcctgttcgt gttcaaaaag gataatgcaa acgcgatcaa
    18661 gaagttcgcg accggatggt tcgggctggt cttcctcgtg tcgctcgcgc tcgtcggata
    18721 cgaccgtgcg gcgggcggga tgcagtttat cgaagacctc cagtggattc ccatcatcgg
    18781 cgcgcgctac cagatgggcg ttgacggcgt ggccgtcctg ctcatcgtcc tgacgactct
    18841 gctcggcgtc atcgccgcgc tctcttcatg ggaatacatc gggaagcgcg aacaggaata
    18901 ctacgtgctc ctgttgctgt tgcaggcggc ggtggtcggc gtcttcgctt cgatggactt
    18961 gttcctcttc tatctcttct tcgaagtctc gctcgtgccg atgtatttcc tgatcggcat
    19021 ctggggc
//
LOCUS       DASWNS010000036         5375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_494043, whole genome shotgun sequence.
ACCESSION   DASWNS010000036 DASWNS010000000
VERSION     DASWNS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494043"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..591)
                     /locus_tag="VGB76_01240"
     CDS             complement(139..591)
                     /locus_tag="VGB76_01240"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEY0097550.1"
                     /translation="MAQEELRQTLEDEIREIVGRETRAWDTKDVRLLLTVFHPDMVWP
                     WPPDARSHDPSTWVFWAGRYNYERWRANWQELFDTHELVHNRREIIKIVLTAEGDGAF
                     AVVDVDTLWRDHAGADFHWQGRACKVYAKVGDEWKMTMHTGLLDYGQL"
     gene            complement(618..1079)
                     /locus_tag="VGB76_01245"
     CDS             complement(618..1079)
                     /locus_tag="VGB76_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097551.1"
                     /translation="MLTQNRRTPFGLLVLACLLFACANETVAQKTCKPRVDKNNIAVE
                     LTIPNFNGGQRTVLSMPAKEKEMRAFGEYVKNQSANHASPVSYSVVITDKNSNGVTLE
                     LNVSTKAANGGYQVTKRTIVLPYETIVEQRYMTGVQVKAYFKLRPFNCKSL"
     gene            complement(1198..1572)
                     /locus_tag="VGB76_01250"
     CDS             complement(1198..1572)
                     /locus_tag="VGB76_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097552.1"
                     /translation="MHGDMHENLLAKLRFWGRHRCPRCRMPLHAEEIACPVCELRIVF
                     LNERGEVMVEMVAVKDPNDYESAEVGEYHLTLALEEAGDMHLPRLHLPTEPQRADLVP
                     LPGDTFGIRFHNRIALEDITRG"
     gene            complement(1604..2710)
                     /gene="hemE"
                     /locus_tag="VGB76_01255"
     CDS             complement(1604..2710)
                     /gene="hemE"
                     /locus_tag="VGB76_01255"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY0097553.1"
                     /translation="MTTKPPTQAATLASDTPTRDNSTSDALYNSPFMRACRREPAPYT
                     PVWLMRQAGRYMPEYREVRARTTFLELCKTPDLAAEVTVYAAERLGVDAAIIFADILL
                     ILEPLGLELEFAKGEGPVIHNPVRTSADVDRLRELETIESLDYVMQAIRTTRRALPPG
                     LPLIGFAGAPFTLASYIIEGGSSRNYIHTKRLMHADKGAWHALMARIARALPLYLNAQ
                     IAAGAQAVQLFDSWVGCLSPEDYREFVLPHTLQAIRNISPGTPVIHFGTGTATLLELM
                     REAGGDVIGLDWRVPLEEGWRRIGYDRAVQGNFDQILLFTSPKIIRQHVGRILQQAAG
                     RPGHIFNLGHGILPETPVDNVVALVEAVHEMSAG"
     gene            2986..3567
                     /locus_tag="VGB76_01260"
     CDS             2986..3567
                     /locus_tag="VGB76_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0097554.1"
                     /translation="MSDTAEALKNPVATPAHQTARDATLGFDEAFMLHHRAVYNTSYA
                     LLRDAGLAEDVTQEVFLRLYRQLDAEPPADEFLRPWLLRVAINLSHNTMRSRTRSVAR
                     EEEFAKQTIGTDGLTAPLDADYERRTEIEEARRALEKIEEPMRSCLLLKQQGLSYREI
                     AATLSINETYVGSLVARGRKEFARVYGKIGGRR"
     gene            3567..4565
                     /locus_tag="VGB76_01265"
     CDS             3567..4565
                     /locus_tag="VGB76_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0097555.1"
                     /translation="METCLDEGTLQAYLDGELAPEMLNGIAVHLSACANCAAAAHAAE
                     QELALFSTAFAPALSANVPTAKLRARLDDAIAELNQPQREFATLPATARLRSFFGSIA
                     VSLKTFTPRQATAFASFLVAVALAVVFFALIATPDKPQATGGEIAKNERPSNERPSNE
                     KPYASVPSAAQTQKPDPTHDSTARLIQSGEVNAPAATARREGRAGSVKANLNVRPQAV
                     ETSVERGSAPEGVNASVAAKVEPMLEDEKTYISSIASLTSAIEAQGAEGLTPTLRAEY
                     ERNLAVVNQAIISSRVAARRNPQDTDAKEFLRTAYQNKVELLSAVADQAQIASSRD"
     gene            4661..>5375
                     /locus_tag="VGB76_01270"
     CDS             4661..>5375
                     /locus_tag="VGB76_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY0097556.1"
                     /translation="MRRNSSYISAVAVCLLLALLACPFSSGTAAPAVRESLAGDRSVH
                     GGGDPTVTAEQTLAAEATVTLLACIESGDVVVRAWDRREVRARSNDASRIELRRADAT
                     SEPNPATRIEVLVAKDEAEPARPGLCSNSGNLELDVPHGSTVQIKGNTGDVSITGIAN
                     VRAKTLSGDIELQRITKSTEAWSANGTIVMRNSSGRVRLETISSEIVASDLGADGASD
                     EFFAKSVSGEISLAKLAHTH"
BASE COUNT         1061 a         1591 c         1695 g         1028 t
ORIGIN      
        1 gcatcacttc ctctcgcgct tcgggtccac cgaaagagac gacgagaagc gcgtcgtacg
       61 actctgccgt tcgtgaatcg ctcatcgaaa tttaaatccc cttgaacctg cgccgccgcg
      121 cacgcgccgc gcgcgagtct ataactgtcc gtagtcgagc agccccgtgt gcatcgtcat
      181 cttccactcg tcgccgacct ttgcatagac tttacaggcg cgtccctgcc agtggaagtc
      241 tgcgcccgca tggtcgcgcc agagcgtgtc cacgtcaacg acggcgaacg ccccgtcgcc
      301 ctctgccgtc aggacgatct taatgatctc gcgccggttg tggacgagtt cgtgcgtgtc
      361 gaacaactcc tgccagttcg cgcgccaacg ctcgtagttg taacgacccg cccagaagac
      421 ccacgtggac ggatcgtgcg agcgcgcatc gggcggccac ggccagacca tgtccgggtg
      481 aaagactgtc aacagcaggc ggacatcttt cgtatcccaa gcgcgcgtct cgcgtccgac
      541 gatctccctg atctcgtctt cgagcgtttg ccggagttct tcctgcgcca taatttttca
      601 gagggcggcg cgccgtctta gagcgatttg cagttgaacg ggcgcagttt gaagtaagcc
      661 ttcacctgca cgccggtcat gtaacgctgc tcgacgatgg tttcgtaagg caggacgatg
      721 gttcgtttcg tcacttgata accaccgttg gcggccttcg tgctcacgtt cagttcgagc
      781 gtcacgccgt tactgttctt gtccgtgatg accaccgaat aggagacggg cgaagcgtgg
      841 ttggccgatt gatttttgac gtactcgccg aaggcacgca tctccttctc tttcgcgggc
      901 atgctcagga cggtgcgctg cccgccgttg aaattcggaa tcgtgagttc gacggcgatg
      961 ttgttcttgt caacgcgcgg tttgcaggtc ttttgggcga ccgtctcgtt cgcgcaggcg
     1021 aacaagaggc aggcgagcac aagtaggccg aagggggttc ttctattttg cgtcagcatc
     1081 aggggatacc tcggggattt aggggtttag gggtttggct cacacggctt gggagcgcgc
     1141 gtgtgaaccc tgaaaaatct ctaacgaact ttcaagctaa cttcaagacg aacaaactca
     1201 accgcgtgta atgtcttcga gggcgatgcg gttgtgaaaa cgtatgccga acgtgtcgcc
     1261 ggggagcggc acgaggtctg cgcgttgcgg ttcggtcggc agatgaaggc gcggcagatg
     1321 catgtcgccc gcctcttcga gcgcgagcgt caaatgatac tcgcccacct ccgcgctctc
     1381 gtaatcgttg gggtctttga ccgccaccat ctcgaccatc acttcgccac gctcgttcaa
     1441 aaagacgatc cggagttcgc acacggggca ggcgatctct tcggcgtgta gaggcatgcg
     1501 acaacgcggg cagcgatgtc gtccccagaa gcgaagttta gcgagcaggt tttcgtgcat
     1561 gtctccgtgc atgtctctta cgagtctaaa aaaaagtctc attctaaccc gcgctcatct
     1621 catgcacagc ttcgaccagc gcgacgacat tgtcaacggg tgtttcgggc agaatgccgt
     1681 gcccgaggtt aaagatgtgg ccggggcgtc ctgcggcctg ttgcagaatg cgcccgacgt
     1741 gctggcgaat tatcttggga gaggtgaaca gaaggatctg atcgaagttg ccctgcacgg
     1801 cgcggtcgta gccgatgcgc cgccaacctt cttcgagcgg gacgcgccag tcgaggccga
     1861 tcacgtcgcc cccggcttcg cgcatgagtt cgaggagcgt cgccgttccc gtcccaaagt
     1921 gtatcacggg cgtgcccggc gaaatgttgc gaattgcttg caatgtgtgg gggaggacaa
     1981 attcgcggta atcttcgggg ctgagacagc cgacccacga gtcgaaaagc tgtacggcct
     2041 gcgcgcccgc cgcgatctgc gcgttgagat agagcgggag cgcgcgtgct atccttgcca
     2101 ttaaggcgtg ccacgcgcct ttgtcggcgt gcatcaggcg tttcgtgtga atgtagtttc
     2161 gagaagaacc gccttcgatg atgtaggagg cgagcgtgaa gggcgcgccc gcgaaaccga
     2221 tcaggggcag tccgggcggc aaggcgcgcc gcgtcgtgcg gatggcctgc atcacgtagt
     2281 ccagactttc gattgtttcc agttcgcgca ggcgatccac gtcagccgac gtgcgcaccg
     2341 gattgtgtat gacagggcct tcgcctttgg cgaattcgag ttcgaggccg agcggttcga
     2401 ggatcaggag gatgtctgcg aagatgatcg cggcgtccac gccgaggcgt tcggcggcgt
     2461 agacggtgac ttcggctgcg aggtcgggcg tcttgcagag ttcgagaaaa gtggtgcggg
     2521 cacgcacttc gcggtattcc ggcatgtagc gtccagcctg tcgcatcaac cagacgggcg
     2581 tgtagggcgc gggttcgcgg cggcaggcgc gcatgaaagg actgttatag agcgcgtcgg
     2641 aagttgaatt atcgcgcgtc ggagtgtcgc tggcgagagt tgcggcttgg gtcgggggct
     2701 tcgtcgtcaa gttcttcagt ccacgttcac gaaacttggg gcagcggcgg ggcgtacaaa
     2761 attgcttgag acggcgacgc cctcctgaaa aatccgtccc gctcagacaa gagcagacta
     2821 caatatgtaa ccccctgagc gcgagtcgcc gcagggcttt ccgcctcatc tgaaccattt
     2881 taatgcgacg tgagtgcgtc gtccgcgaga tcgggagcgg cgggcgcgaa attatttgcc
     2941 acaaacgaag cgcgggcggt gtttacagtg atgagagcgt cgaagatgag cgacacagca
     3001 gaggctctaa aaaatccagt ggctaccccc gcccaccaga ctgcgagaga cgcgacgctc
     3061 ggctttgacg aggcatttat gttgcaccat cgcgccgtct acaacacgtc atacgcgctt
     3121 ttgcgcgacg ccgggctggc cgaagacgtg acgcaggaag ttttcctgcg gctctatcgc
     3181 caactcgacg cagagcctcc ggcggacgag tttttgcgcc cgtggctgct ccgcgtggcg
     3241 atcaatttgt cgcacaacac gatgcgcagc cgcacgcgtt cggtggcgcg cgaagaggag
     3301 tttgcgaagc agacaatcgg gacggacggg ctgaccgccc cgctcgacgc agattacgaa
     3361 cgccgcacag agattgaaga agcgcgccgc gcgctcgaaa aaattgagga gccgatgcgt
     3421 agttgcctct tgctcaagca gcagggcttg tcttatcgcg agatcgctgc caccctgtca
     3481 atcaacgaaa cttacgtggg cagtctcgtg gcgcgcgggc gcaaggagtt cgcacgggta
     3541 tatggaaaga tcggaggacg ccggtgatgg aaacctgtct cgatgaagga acgctgcaag
     3601 cttatctcga cggcgaactc gctcccgaaa tgctgaacgg catcgccgtg catctctccg
     3661 cctgcgccaa ctgcgccgcc gccgcacacg cggcggaaca ggaactcgcg ctcttctcca
     3721 cggcctttgc ccccgcgctc tctgcgaacg tgccgacggc gaaactgcgc gcacgcctcg
     3781 acgatgcgat tgcggagttg aaccagccgc aacgcgaatt tgcgacgctg cccgccaccg
     3841 cgcgcctgcg ctctttcttc ggctccatag ccgtctcttt aaagaccttt acgccgcgtc
     3901 aggccaccgc ctttgcgagt ttcctcgtcg ccgtcgcgct cgccgtcgtc ttcttcgcgc
     3961 tgatagccac gccggataag cctcaagcga caggcggaga aattgcgaaa aacgaacgac
     4021 cttcaaacga acgaccttca aacgaaaaac cttacgcgtc cgtaccttcc gccgcgcaga
     4081 cccagaagcc cgatccgacg cacgacagca cagcgcggtt aatccagagc ggagaagtta
     4141 acgctcctgc tgcgaccgcg cggcgcgaag ggcgtgcagg ctccgtgaag gccaatttga
     4201 atgttcgtcc gcaagccgtg gagacgagcg tggagcgggg cagcgcgccg gaaggcgtga
     4261 acgccagcgt cgcggcgaag gtcgagccga tgctggaaga cgaaaagact tatatcagtt
     4321 ccatcgcttc gctcacttcc gccatcgagg cgcaaggggc ggaggggctg acgccgactc
     4381 tgcgcgccga gtatgagcgc aatctggcgg tcgtcaacca agcgatcatc tcgtcgcgcg
     4441 tggcggcgcg gcgcaacccg caagacacgg acgcgaaaga gtttttgcgt accgcttacc
     4501 agaacaaggt cgagttgttg agcgcggtgg ccgatcaggc gcaaatcgcc agcagccgcg
     4561 attaactcgc tcagtccgac gtgattgatc tcacacattt caagcggcgc gctggccttg
     4621 atatcaacgc cgccggatta ttaaaggaac tttttacagg atgagacgga atagctctta
     4681 tatctcggca gtcgccgtgt gcctgttgct cgcgttgctg gcgtgcccgt tcagttcggg
     4741 aaccgccgcg cccgccgtgc gtgaaagttt agcgggcgac cggagcgtgc acgggggcgg
     4801 cgacccgacc gtgacagccg aacagaccct cgcggcggag gcgaccgtga cgcttttggc
     4861 ctgtatcgaa tcgggcgacg tggtggtgcg cgcatgggat cgcagagagg tgcgcgcccg
     4921 ctcgaacgac gcctcgcgca tcgaactgcg ccgcgccgac gccaccagcg agccgaatcc
     4981 ggcgacgcgc atcgaagtgc tcgtcgctaa agacgaggcc gaacccgcgc gccccggcct
     5041 gtgcagcaat tccggcaatc tggaactcga cgtgccgcac ggcagcaccg tccagatcaa
     5101 aggcaacacg ggcgacgtga gcatcacggg catcgccaac gtgcgggcga agaccttgag
     5161 cggcgacata gagttgcagc gcatcacgaa gtcaaccgag gcttggagcg cgaacggcac
     5221 gattgtgatg agaaattcga gtgggcgcgt gcgtctggag acgatcagca gcgagatcgt
     5281 ggcaagcgat ctcggggcgg acggggcgag cgacgagttt ttcgccaagt ctgtgagcgg
     5341 cgaaatctcg ctcgccaagc tggcgcacac gcacg
//
LOCUS       DASWNS010000037         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_260818, whole genome shotgun sequence.
ACCESSION   DASWNS010000037 DASWNS010000000
VERSION     DASWNS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260818"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VGB76_01275"
     CDS             <1..296
                     /locus_tag="VGB76_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097557.1"
                     /translation="LLRAAEDLSDTKRKEEYEPRLDVLETLIRDAWLLSLDPKTKIVN
                     EDIRERLARFGAQLESRRVADWMTRIQNLRGQLAVNINRRVATDALFLSMAGA"
     gene            complement(395..1504)
                     /gene="ispG"
                     /locus_tag="VGB76_01280"
                     /gene_synonym="gcpE"
     CDS             complement(395..1504)
                     /gene="ispG"
                     /locus_tag="VGB76_01280"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY0097558.1"
                     /translation="MRETRAVRIGNVQVGGGAPVAVQSMTKTDTSDVDGTVKQIEEML
                     SAGCEIVRLAVPDKEAALALKEIRRRVPQVPLVADIHFHYKLALMALDAGVDKLRLNP
                     GNIGAHERVREVTRAAQSQNVPIRIGVNGGSLEKDLLARYGTATPEAMVESALRHIRI
                     LEDLNFNDIIVSLKASDVHRTVAAYRLLSAQVDYPLHLGVTEAGTAFSGSIKSSIGLG
                     ILLHEGIGDTIRVSLAAEPHEEVRVAWEILKSLELRQRGVTVVACPTCGRLDVDDFVG
                     IVTEIERRLAHIEEPLHLSIMGCAVNGPGEAHDSQLGVTFGRGVGMIYKDGKPMRKVA
                     GADIVEAFVAETEKLVAEGASAKTQETPELVTIER"
     gene            complement(1568..>2656)
                     /gene="rseP"
                     /locus_tag="VGB76_01285"
     CDS             complement(1568..>2656)
                     /gene="rseP"
                     /locus_tag="VGB76_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY0097559.1"
                     /translation="QKFLVMVAGPTMNILTALAVFWAGAMISGIPATPTSPVIRYMKP
                     GGVAEAAGLRAGDRIVSFNGKENPSWDRIRGDVSLSPEQPLPMVVERNGQRVDLTIKP
                     TRYTEEGESIGEIDFRLDYGVHPVMVTEVIAGSPAEQAGLRANDRVVAVNNEAVRDHF
                     DVLAFVQAHRNEAVRLTVEREGQRQELTAQTNAEGKLGFVPTTLLPQRSVGPVAAFQH
                     SVNRNLEILRMTGAAFAQIFRGQRSARESLSGPIGIARASSTAANKLGWAGVFTMLGF
                     LSLNLGVVNLLPIPVLDGGAIFLLWIEAILGWIGVKMSMTVRERIQQVGMVMLLLLMG
                     FVITNDLLKEVSRLGASDDKPAATQPAK"
BASE COUNT          497 a          857 c          794 g          508 t
ORIGIN      
        1 gcctgcttcg cgccgccgag gatttgagcg acactaagcg caaggaagag tacgaacccc
       61 ggctcgacgt gctcgaaacg ctcatccgcg acgcgtggct cctctcgctc gaccccaaaa
      121 caaagatcgt caacgaagac atacgcgaac ggctcgcacg cttcggcgcg caactcgaaa
      181 gccgccgcgt cgccgactgg atgacgcgca tccagaattt gcgcggccaa ctcgccgtca
      241 acatcaaccg ccgcgtcgcc accgacgccc tgtttctctc gatggccggg gcgtagaaaa
      301 ggcgcgagcg cacaaggcaa aaggttaggg gcagaggaaa gaccgtctta cggcgtctcc
      361 ctctgcccct gacctttaat cctgtgccct gcttttatct ttcgattgtg acgagttcgg
      421 gcgtctcctg cgttttcgcc gacgcgcctt cggccaccag tttttccgtc tccgcgacga
      481 aggcttccac gatgtctgcg cccgccactt tgcgcatcgg tttgccgtcc ttgtagatca
      541 tgccgacgcc gcgcccgaag gtgactccga gttggctgtc gtgcgcctcg cccggtccgt
      601 tgacggcgca gcccatgatc gagagatgca gaggctcttc gatatgcgcc agccgacgct
      661 cgatctccgt cacgatgccg acgaaatcat ccacgtccag ccgcccgcac gtcgggcacg
      721 ccacgaccgt cacgccgcgc tggcgcaact ccagagattt caaaatctcc cacgccacgc
      781 gcacctcttc gtgcggttcg gcggcgagcg agacacgaat cgtatcgccg atgccttcgt
      841 gcaaaaggat gccgaggccg attgatgatt tgatcgaccc gctgaaagcc gtgcccgctt
      901 ccgtcacgcc gagatgcaac gggtaatcaa cctgcgcgga gagcaagcga tacgcggcca
      961 ccgtgcgatg cacatccgat gccttgagcg agacgatgat gtcgttgaaa tttaggtctt
     1021 cgagaatgcg tatgtggcgc aaggccgatt cgaccatcgc ttccggcgtc gccgtgccgt
     1081 atcgcgccag taagtctttt tcgagcgagc cgccgttgac gccgatacga atggggacgt
     1141 tttgcgattg cgcggcgcgc gtcacttcgc gcacgcgttc gtgcgcgccg atgttgccgg
     1201 gattcaggcg caacttatcg acgcccgcgt ccagagccat cagagccagt ttgtaatgga
     1261 agtgaatgtc cgcgacgagc ggcacttgcg gcacgcgccg cctgatctct ttgagcgcga
     1321 gggcggcttc cttgtccggc acggcgagcc ggacgatctc gcagcccgcg ctcaacatct
     1381 cttcgatctg tttgaccgtg ccgtcgacat cggacgtgtc cgtcttcgtc atcgactgca
     1441 cagcgacggg cgcgccgccg ccaacctgca cattcccgat cctgaccgct ctcgtctctc
     1501 tcataaagtc acaagtccag agtctcaagt cccaggtcgc aaaccgttgt gacgtgggac
     1561 tcgaaaccta tttcgccggt tgtgtcgccg ccggtttgtc gtccgaggct cctaagcgcg
     1621 aaacctcttt gagcaggtcg ttggtgatga cgaatcccat caggaggagc agcatcacca
     1681 tgccgacttg ctgtatgcgc tcgcgcacgg tcatcgacat cttgacgccg atccagccga
     1741 ggatcgcttc aatccacaac aggaagatcg cgccgccgtc gagcacgggg atcggcagca
     1801 agttgacgac gccgagattc aggctcagga agccgagcat cgtgaagact cccgcccaac
     1861 ccagtttatt ggcggcggtg gaagacgcgc gggcgatgcc gattggtccg gagagcgatt
     1921 cgcgcgccga acgctggccg cgaaagatct gcgcgaaggc tgcgcccgtc atgcgcagaa
     1981 tttcgagatt gcgattgacg gaatgctgga acgcggccac gggaccgacg ctgcgctgcg
     2041 gcaggagcgt cgtcgggacg aaaccgagtt tcccttcggc gttagtctgc gcggtgagtt
     2101 cctgtcgctg cccttcgcgt tcgacggtca ggcgcacggc ttcgttccgg tgtgcctgaa
     2161 cgaaggcgag cacgtcgaag tggtcgcgca cagcctcgtt gttcaccgcc accacgcggt
     2221 cgttcgcacg caagcccgcc tgttcggcag gcgacccggc gatcacctcc gtaaccatca
     2281 cgggatgtac gccgtagtcg agacgaaaat caatttcgcc gatgctctcg ccctcttcgg
     2341 tatatctggt cggcttgatg gtcaagtcca cgcgttgacc gttgcgttcc acgaccatcg
     2401 gcagaggttg ttcgggcgag aggctcacgt cgccgcgaat ccggtcccag gaaggattct
     2461 ctttgccgtt aaaggagacg atgcggtcgc ccgcacgcag gccagccgct tcggcgacgc
     2521 cgccgggctt catgtagcgg atgacgggcg aggtcggagt agcgggaatg ccggagatca
     2581 tcgcgcctgc ccagaagacc gcgagcgcgg tcaaaatatt catcgtcggc cccgccacca
     2641 tgacgagaaa cttctg
//
LOCUS       DASWNS010000038         8235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_105103, whole genome shotgun sequence.
ACCESSION   DASWNS010000038 DASWNS010000000
VERSION     DASWNS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105103"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..1496
                     /locus_tag="VGB76_01290"
     CDS             414..1496
                     /locus_tag="VGB76_01290"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY0097560.1"
                     /translation="MNILSNFFSDDLAIDLGTVNTLIYAPDRGIVLNEPSVVAIHKYT
                     GEVLSVGAEAYKMVGRAPKDAEVFRPVKGGTVDNFEAAEKMLAAFIRRVHGNQNKRSH
                     MVIGVPGSSTALEQRSVRDAARDAKATRVDLVDEGLAAALGAGLGYEDERAHLIVDIG
                     GGTTNIAIISSAAVVSSSSLKVAGNAMDDAIRDYVRAKYEMQLGERTAEQVKKELGAG
                     HAEARVDGKREARHMEVVGKELTSGLAKAVELSSDEVRQALEPVLSEIVAGARRAIED
                     SQPDVTADIYQTGLILTGGGALLEGMAERLQNELRLHVTVADDPLAAVALGAGRLLAD
                     AERLQRAAIREDVPAWQGSEELVVNW"
     gene            complement(1561..2352)
                     /locus_tag="VGB76_01295"
     CDS             complement(1561..2352)
                     /locus_tag="VGB76_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097561.1"
                     /translation="MKTQRERNQILLRGLLLPTIFLTVALMGGLRVAAETQAFLFVAP
                     PLVALVLAVLLSLLFVRGRLVNLHAWFAGDHAPLTLISHALTLLALFFASAQAFTSVL
                     PERGLFRWMFAFFFLWTLWHNQFANFDARLTLRSLVALFGTAFVLKHILLASLYAPDG
                     GWLKRLAGALLEGFTQGTLGEQQTFAPATGYISFFTLALYVLGLVLLPPAPQPGDASP
                     ERLDAHELVTQYRQLSLEERALLREEMLRTERGLLEDEIHATRTD"
     gene            complement(2400..2906)
                     /locus_tag="VGB76_01300"
     CDS             complement(2400..2906)
                     /locus_tag="VGB76_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097562.1"
                     /translation="MQTTLIKSAGIAWTLLYAAAIIWIYATAPRTFTEATTGVSVAAG
                     TYAIDEARFLAARELFRREQYAAARDEWMRADPAMRDARTQFYIAYAFYREGWGRVYH
                     DDALYKQGLEAVARALALSSAEGGALAVADPELKIHSAAELKAELQQGLETSTGDFNP
                     LKIMRERK"
     gene            2983..3510
                     /locus_tag="VGB76_01305"
     CDS             2983..3510
                     /locus_tag="VGB76_01305"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HEY0097563.1"
                     /translation="MKTFLLSLLFVVAASGVAAAQEQKSHDSHKKHDAAKVETAKVER
                     DGDVIKRGVALGTETPAVKFADVLKEPAKFAGQRVRIEGVVERVCQSEGCWMQITPEA
                     GVAGIRVTFKDHSFFVPKDSKSMKFKAEGEFFVKVLDKAQVDHLVEDGAKIERKPDGT
                     ADEIRFVATGVELWK"
     gene            3596..4666
                     /gene="dusB"
                     /locus_tag="VGB76_01310"
     CDS             3596..4666
                     /gene="dusB"
                     /locus_tag="VGB76_01310"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY0097564.1"
                     /translation="MLPFKVRDVLISPPLVLSPMAGVTDVAFRGLLKRCGGVGLTVSE
                     FISVEGLTRNNPKSKRQMRFYDYERPFAVQIFGGQPERMRMAAEMAEECGADVLDVNC
                     GCPAPKVVKHGGGSGLLRDMPRLETILKEIKRSITIPLTIKIRAGYYDNNINAVETAR
                     LAEACGVEHIALHGRTKEQGYKGRANWDLVRQIKETVKIPVSGSGDVTTVDEALARWR
                     ETGCDGILIGRGAMANPWVFRQIEDTLAGRAPFQPTLADKRNLLLEYFELLRADMPEL
                     PAIGRMKQLAGQFTRGLVGGSRFRQVLYHSHAVDEILERIEEYFETVAAGRVYIGEGD
                     ASEAEEEGAASPVLDSCEAATV"
     gene            4736..4918
                     /locus_tag="VGB76_01315"
     CDS             4736..4918
                     /locus_tag="VGB76_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097565.1"
                     /translation="MTEHREETRDEEGHFNEQDRVDERGDVAEPDAPVTAEHNEGMST
                     ILDGEPGVGVQDSLGE"
     gene            complement(5037..5297)
                     /locus_tag="VGB76_01320"
     CDS             complement(5037..5297)
                     /locus_tag="VGB76_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097566.1"
                     /translation="MARGWESKAVEDQIQEAEERARRAAEPPVDESPAARARRERLET
                     LRLSRSRTLSQLEQATRRPHREMLQRTLRALETEIEELQSAE"
     gene            complement(5382..6623)
                     /locus_tag="VGB76_01325"
     CDS             complement(5382..6623)
                     /locus_tag="VGB76_01325"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0097567.1"
                     /translation="MAKKQARILIIGGGFGGLFTALDLAGDGEVTLVSAEDHFTFRPM
                     LYEYLSGEVEAWHIAPHYRELLDDKIRFIHGAATDVDFEARRVVIEGWAEPQSYDVLV
                     LAVGGVTNYAGVEGAQEYALPFRGIADADALRNRMIETLDRIPPEASAEEAQRAATFA
                     VVGAGASGCELSTKMGDLLRDAFRRRGLQGAPRVVVVEMAEHVVQGMGEELRVYVEEA
                     LAEAGVEVHTQTRVRRVTQEGFTFEQAGVQTELAAAAVVWVGGVKVSPLVEHLQIPKE
                     RRGLVSVEPTLQVEGRREVFSLGDDARFAAASPQLAGTAQLAFQQASLAAANIRALLG
                     GHELKTKHFEEMGEALSLGTERAAVLTAGKAFGGALARQARFALYTSRLPTWHHRVKV
                     GASWFFEGTAPLPLGLRRPGT"
     gene            complement(6731..7093)
                     /locus_tag="VGB76_01330"
     CDS             complement(6731..7093)
                     /locus_tag="VGB76_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0097568.1"
                     /translation="MPLTILYVEDHKVVAHVVRETSKAEGWCVVLCYDGAVAVKRLAS
                     AASYDLLLFDNDLPNVSGLELVRYAREFPHRTQTTIIVLSAGACRRDARLAGANEFLR
                     KPEDIGKIVETVTPLLTS"
     gene            7309..7620
                     /locus_tag="VGB76_01335"
     CDS             7309..7620
                     /locus_tag="VGB76_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0097569.1"
                     /translation="MGSARERPARLAEKLLQIRTALGISQGEMLRRLGADEKSDRYYV
                     SNYETGKREPSLLVLLEYARIANVLMEVLADDELDLPETIPSSTRSEGIRRTPVNSQR
                     R"
     gene            7792..8058
                     /gene="rpsO"
                     /locus_tag="VGB76_01340"
     CDS             7792..8058
                     /gene="rpsO"
                     /locus_tag="VGB76_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY0097570.1"
                     /translation="MLDRESKEQIVTTYQTHEGDTGSPQVQVALLTTRINQLTEHFKT
                     HKKDNHSRRGLLKMVSQRRSLLDYLKRRDIEQYHKLISSLGLRR"
BASE COUNT         1651 a         2462 c         2599 g         1523 t
ORIGIN      
        1 cgtccttcca agcggaaagc cgcacgcgtc aacaactgaa ctggccgtta caaatcgtcg
       61 gcgatggagc cattcatcgc cgactttttt tcgcgcggcg tactaactgc ggcatcgagt
      121 aattgttacc cgcctgacgg acgaagcgcg tctatttttc atccctcatc ctgcgccctt
      181 catcgctgct tttcgcgcgt tatccctgct ttttgggcgt tcgtctttcg cccgcccttt
      241 tttttaatgt cttagaaaca gacaccggcg gggaacgccc cggttgaatt tacgggcatt
      301 tcaggtggcc tcccggtggt atgcggtttg ccgtttggag aggcgcacgc gtctatcaaa
      361 ctctcctcag tggaacaaga gggttattcg gagtctatca ggggcaagct attatgaaca
      421 ttttaagtaa cttcttctcg gacgatctcg ccatcgacct cggcacggtg aacacgctca
      481 tctacgcgcc cgaccggggc atcgtcttga atgagccttc ggtggtggcg attcacaagt
      541 acacgggcga agtgttgtcg gtgggtgcgg aagcgtacaa gatggtgggg cgtgcgccga
      601 aagatgcgga ggtgtttcgt ccggtcaagg gcggcacggt cgataacttc gaggccgcag
      661 agaagatgct cgccgccttc attcgccgcg tgcacggcaa tcagaacaaa cgcagccaca
      721 tggtgatcgg cgtgcccggc tcttccaccg cgctcgaaca acgctccgtg cgcgatgcgg
      781 cgcgcgacgc taaagctacg cgcgtcgatc tcgttgacga aggactggcc gccgcgctcg
      841 gcgccggact cggctacgaa gacgaacgcg cgcacttgat cgttgacatc ggcggcggca
      901 caacgaacat cgccatcatc tcttcggcgg cggtcgtctc ttcgagcagt ttgaaggtgg
      961 cgggcaatgc gatggacgat gcgatccgcg attacgtgcg cgcgaagtat gagatgcagc
     1021 tcggcgagcg caccgccgaa caggtcaaga aagaactcgg cgcggggcac gcggaagcgc
     1081 gcgtggacgg caaacgcgaa gcgcgacaca tggaagtggt cggcaaggaa ctgacgagcg
     1141 ggctggcaaa ggcggtcgaa ctcagttccg atgaagtgcg gcaggcttta gagccggtgc
     1201 tctccgagat cgtcgcgggc gcacgccgcg cgattgaaga ctcgcagcca gacgtgacgg
     1261 cggacatcta ccagacggga ctcatcctga cgggcggcgg cgcgcttctc gaaggcatgg
     1321 ccgaacgcct ccagaacgaa ctgcgcctgc acgtcacggt cgcagacgat ccgctcgcgg
     1381 cggtcgcgct cggcgcggga cgcctgctcg ccgacgcaga gagactccaa cgcgccgcca
     1441 tccgcgaaga cgttcccgca tggcaaggct ccgaagagtt ggtcgtcaac tggtaatcga
     1501 ataagcggtc gagatcactg aggcgggagg acaggcgcag agagcgttcg tcctcccgcc
     1561 ttaatctgta cgggtagcgt gaatctcatc ttcgagcagc ccgcgttccg ttcgcaacat
     1621 ctcttcgcgc aggagtgcgc gttcttcgag cgacagttgg cggtattgtg tgacgagttc
     1681 gtgcgcgtcc aggcgttcgg gcgacgcgtc gccgggttgg ggcgcgggcg ggagcaggac
     1741 gaggccgagg acgtagaggg cgagcgtgaa aaagctgata tagccggtgg cgggcgcgaa
     1801 ggtttgctgt tcgccgagcg tgccttgcgt gaagccttcg aggagcgcgc ccgcgagtcg
     1861 tttgagccag ccgccgtcgg gcgcatagag cgaggcgagc aggatatgtt tgaggacgaa
     1921 ggccgtgccg aagagggcga cgaggctgcg cagggtgagg cgcgcgtcga agttggcgaa
     1981 ttgattgtgc cagagcgtcc agaggaagaa gaaagcgaac atccagcgga acaggccgcg
     2041 ttcgggcagc acgctcgtga aggcttgagc ggaggcgaag aagagcgcga gcagcgtgag
     2101 cgcgtgcgag atgagcgtga gcggagcgtg atcgcctgcg aaccacgcgt ggagattgac
     2161 gagacgcccg cgcacgaaga gcagcgacag gaggactgcg agcacgagcg cgacgagcgg
     2221 cggcgcgacg aagagaaacg cctgtgtctc cgccgcgacg cgcagaccgc ccatgagcgc
     2281 gacggtcaaa aagatcgtcg gcaacaagag gccacgcagg agaatttgat tgcgttcgcg
     2341 ttgagtcttc atcggtgcaa agttaattcg aggggcgacg ctcgctgcgt gggcgtcgct
     2401 cattttcgct cgcgcatgat cttgagcggg ttgaaatcgc ccgtggatgt ctccaatcct
     2461 tgctgcaact cggccttgag ttcggcggcg gaatgaatct tcaactccgg gtcggcgacg
     2521 gccagtgcgc ctccttcggc ggacgacaga gcgagcgcgc gtgcgaccgc ttccaagccc
     2581 tgtttgtaca aagcgtcgtc gtgatagacg cgcccccatc cctccctgta gaaagcgtag
     2641 gcgatgtaga actgcgtgcg cgcgtcgcgc atggccgggt cggcgcgcat ccattcgtcg
     2701 cgcgccgccg cgtattgctc gcggcgaaac agttcgcgcg ccgcgagaaa gcgcgcctcg
     2761 tcaatcgcat aagtcccggc ggcgacgctc accccggtcg tcgcttcggt gaaagtgcgc
     2821 ggcgcggtgg cgtaaatcca gatgatggcg gcggcgtaga gaagcgtcca tgcgatgcct
     2881 gcgcttttga tgagggtggt ttgcattcta taatctgacg ccgccaaatg ctcaaatgat
     2941 gcttcggcca atttcagtta caaaggatag cttatttccc ttatgaagac atttctcctt
     3001 tcactcctgt tcgtcgtcgc cgcgtccggc gtcgccgccg cgcaggaaca gaagtctcac
     3061 gactcgcata agaagcacga cgccgcgaaa gtcgaaaccg ccaaagtcga gcgggacggc
     3121 gacgtgatca agcgcggcgt tgcgctcgga accgagacgc ccgccgtcaa attcgcagac
     3181 gtgttgaagg agcctgcgaa gtttgcaggc cagcgcgtgc ggatcgaagg cgtggtggaa
     3241 cgcgtctgcc agagcgaagg ctgctggatg cagatcacgc cggaagcggg cgtggccgga
     3301 attcgcgtca ccttcaaaga ccacagtttc ttcgtgccga aggattccaa gagcatgaag
     3361 tttaaggccg aaggcgagtt cttcgtgaag gtgctggaca aggcgcaggt ggatcacctc
     3421 gtcgaagacg gcgcgaagat tgagcgcaaa cccgacggca cggcggacga gattcgcttc
     3481 gtcgccacgg gcgtcgaact ctggaaataa gtatgaacgc ggaacaatgg acgatgaatg
     3541 aaaagcagac tgtctttcag ttcaccgttc cgcgtccctc cttcatcgtt tagttatgct
     3601 gcctttcaaa gtcagggatg tgttgatctc gccgccgctc gtgctgtcgc cgatggcggg
     3661 cgtgacggac gtggcctttc gcggtctgtt gaaacgctgc ggcggggtcg gcctgaccgt
     3721 ctcggagttc atttcggtcg agggcttgac gcgcaacaac ccgaagtcga aacggcagat
     3781 gcgtttttac gattacgagc gaccgttcgc cgtgcagatt ttcggcgggc agccggagcg
     3841 catgcgcatg gccgcagaga tggccgagga gtgcggcgcg gacgtgctcg acgtgaactg
     3901 cggttgcccc gcgccgaagg tcgtcaaaca cggcggcggc tcggggttgc tccgggacat
     3961 gccgcgcctc gaaaccatcc tcaaggagat caaacgctcg atcacgattc cgctcacgat
     4021 caagattcgc gcgggctatt acgacaacaa tatcaacgcc gtcgagacgg caaggctggc
     4081 ggaagcgtgc ggcgtcgagc acatcgcgct gcacgggcgc acgaaggagc agggctacaa
     4141 ggggcgcgcg aattgggact tggtacggca gatcaaagag acggtcaaaa ttcccgtctc
     4201 cggcagcggc gatgtgacga ccgtggacga agcgttggcg cgctggcgcg agacgggctg
     4261 cgacggcatc ctcatcgggc gcggcgcgat ggcgaacccg tgggtctttc gacagatcga
     4321 agacacgctc gccggtcgcg cgcccttcca gccgacgctc gccgacaagc gcaacctcct
     4381 gctcgaatat ttcgaactgc tgcgcgcgga catgcccgaa ctgcccgcca tcggtcggat
     4441 gaagcaactc gcgggacagt tcacgcgcgg cctcgtcggc ggctcgcgct ttcgtcaggt
     4501 gctctatcac tcgcacgccg tggacgagat actcgaacgc atcgaagaat atttcgagac
     4561 ggtcgccgcc gggcgcgtct atatcggtga aggcgacgcg agtgaggccg aagaagaagg
     4621 ggcggcgtcg cccgtgctcg attcatgcga ggcggcgaca gtgtgaagcc tgtcagttgt
     4681 acgtggttag ttgtccgttg tttgtgatcc gtagaatcga agggagcaag cgggaatgac
     4741 cgagcacagg gaagagacgc gcgacgaaga agggcacttc aacgaacagg atcgcgtgga
     4801 cgaacgcggc gacgtggcgg agccggatgc gcccgtcacg gcggaacata acgaaggcat
     4861 gagcacgatc ctcgacggcg aaccgggcgt cggcgtgcag gacagtctcg gcgagtaata
     4921 atttcgcgca aagcacttaa cgcaaaggcg cgtgcatcag gcggaggggc gcagagagtt
     4981 gaagcttcaa ctctctgcgc ccttttgcgc tctctacgtg tgcggcgttg agactcttat
     5041 tccgccgact gtaattcttc gatctcggtt tctagcgcgc ggagcgtgcg ttggagcatt
     5101 tcgcgatggg ggcgtcgggt ggcctgttca agttgcgaga gggtgcgtga gcgcgagagg
     5161 cggagggttt cgagtcgttc gcgacgcgcg cgtgctgcgg gcgattcgtc tacgggcggc
     5221 tccgccgcgc gacgcgcacg ttcctccgct tcctgtatct ggtcttcgac cgctttgctt
     5281 tcccacccgc gcgccatcgg ccacgctccc ctttacagtt tcaatcaaga agacttactg
     5341 cggtcgcctg ctctcaggcg actcggtgtt cgcgcccgaa tttatgtgcc cggcctgcgc
     5401 agtccgagcg gcagcggcgc ggtgccttcg aagaaccagc tcgcgccgac tttgacgcgg
     5461 tgatgccacg tcggcaggcg cgaagtgtag agcgcgaagc gcgcctgacg cgcgagcgcg
     5521 ccgccgaagg ctttacccgc cgtgagcact gcggcgcgtt ccgtgccgag gctcagggct
     5581 tcgcccatct cctcgaaatg cttcgtcttg agttcatggc cgccgagcag cgcgcggata
     5641 ttggcggcgg cgagcgacgc ttgttgaaag gccagttgcg ccgtgcccgc cagttgcggg
     5701 ctggctgcgg cgaagcgcgc gtcgtcgccg agcgagaaga cttcccggcg tccctccact
     5761 tgcagcgtcg gctcgaccga gacgaggccg cgcctctcct tcggaatttg caggtgctcc
     5821 acgagcgggc tgactttcac cccgcccacc cagacgactg ccgccgccgc cagttccgtc
     5881 tgcacgcccg cctgctcgaa ggtgaaaccc tcctgcgtca cgcgccgcac gcgcgtctgc
     5941 gtatgcacct cgacgcccgc ttccgccaga gcctcttcca cgtagacgcg cagttcctcg
     6001 cccatcccct gcaccacatg ttccgccatc tccaccacga cgacgcgcgg cgcgccctgc
     6061 aacccgcgcc gccggaacgc atcgcgcagc aggtcgccca tcttcgtaga gagttcgcac
     6121 ccgctcgcgc ccgcgccgac gacggcaaag gtcgccgcac gctgcgcctc ttccgcagag
     6181 gcttcgggcg gaatgcggtc gagcgtctca atcatgcgat tgcgtaaagc gtccgcgtcg
     6241 gcgataccgc gaaaaggcag cgcgtactcc tgcgcgccct ccacgcccgc gtagtttgtg
     6301 acgccgccga cggccagcac gagcacgtcg taactttgcg gctccgccca tccttcgatg
     6361 acgacgcgac gcgcctcgaa atccacgtcc gtcgccgcgc cgtgaatgaa gcgaatttta
     6421 tcgtcgagca attcacgata atgcggcgcg atgtgccacg cctccacctc gccgctcaaa
     6481 tactcgtaga gcatcggtcg aaacgtaaaa tgatcctccg cgctcacgag tgtcacctcg
     6541 ccgtcgcccg cgaggtcgag cgcggtgaac aacccgccga agccgccgcc gatgatcaaa
     6601 atacgcgcct gtttcttcgc cataagttta acttgtccgg acgctccttt cgggcgggca
     6661 tggtagcaga gaagcgacgc gcgcgccacg cctctcttta gagggtagcg ggtcggtttg
     6721 aaatccggct ctaagaagta agcagggggg tgacggtttc aacaatcttg cctatgtctt
     6781 ccggcttccg taaaaactca ttcgcgcccg ccaaccgcgc atccctccta catgcaccag
     6841 cagagagcac gataatcgta gtctgtgtgc ggtgtggaaa ttcgcgggca tatcggacta
     6901 gctcaaggcc gctgacgttc ggtaagtcat tatcgaagag caacaaatca taactggccg
     6961 cgcttgccag ccgcttaact gcaaccgccc cgtcgtagca gagcacgacg caccagcctt
     7021 ccgctttcga cgtctctcta actacgtgcg cgacaacttt gtgatcttcg acatagagga
     7081 tcgtgagggg catcttgtgc tatcttgctc gcgctctttc tttgaaaact gctggcaacg
     7141 gtcgcatgtc tagctcaccc tcacattctc acgagtgatg agcgtcgcca cgaagcagag
     7201 actttctact ctcttggcga caacaactgg tgagacgatt ttagttacag ttgtcactaa
     7261 aatcaagcga cgagcgcact tcattgtctc atcaggggag aaatcctgat gggcagcgcg
     7321 cgtgagcgac ccgcacggct cgctgaaaag ttgctccaga ttcgcaccgc actcggtatt
     7381 tcgcagggcg aaatgctcag gcgtctgggt gcagatgaaa agagcgaccg ctattacgtc
     7441 tccaattacg aaaccggaaa gcgcgagccg tcgctgctcg tcctcttgga atacgcacgc
     7501 atcgccaacg tcctcatgga agtcctcgcc gacgatgaac tagacctccc cgaaaccatc
     7561 cccagctcca ccagaagcga aggcatccgg cgcacgccag tcaacagcca gaggcgttag
     7621 tcttctgaaa tagcgccgtt tccaagctca tctctgctgt gcggcgtggt ggctggacag
     7681 ccggtttgtg ggcgtgttaa attgtcgcgg ttttgcttaa ctcttgcggt tgcctgaaat
     7741 tacgggcgaa ctgcggtctg ggagggccat ccggggcagg aagctacagt tatgttagat
     7801 cgagaatcga aggaacagat cgttacgacg taccaaacgc acgagggcga cacggggtcg
     7861 ccgcaggtgc aagtcgcgtt gctcaccaca cgcattaacc agctcaccga gcacttcaaa
     7921 acgcacaaga aagataacca ctcgcgacgc ggactgctca agatggtttc gcagcgacgc
     7981 agcctgctcg attacctcaa acgcagggat atcgagcagt accataaact gatcagcagc
     8041 ctcggcctgc gccgctaaac aaaagcgcgc cagaccttaa aaggcggttg aacggagcat
     8101 atcagcgaag gaaaacggtt ccttccgaca tcaagaaacg cccgcgtccc tcgaaaagcc
     8161 gggtggcagt gccgaacgtg acggcgcaaa ctgtcgccaa gcggacgcga gtagcctgta
     8221 gcagttagtt cagca
//
LOCUS       DASWNS010000039        13859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_209164, whole genome shotgun sequence.
ACCESSION   DASWNS010000039 DASWNS010000000
VERSION     DASWNS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13859
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209164"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4061)
                     /locus_tag="VGB76_01345"
     CDS             complement(<1..4061)
                     /locus_tag="VGB76_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0097571.1"
                     /translation="MSNILESLNNLSPEKRALLALRLKLKGAAASRVQKIPRRENNEA
                     IPLSYAQQRLWFIWKMEPDSIEDNIAVALRITGPLNVQGMERTFSELINRHEILRTNF
                     PEVDGLPIQRINPPQELRLPLVDLSELDEASREARAEQLISEEERRPFDLMNGRMLRV
                     GLVKMHEQEHLLLLTMHHVVSDGWSMSILIREVSTLYDAYSKGNSSSLPELPIQYADY
                     ALWQRAQLSGDALDGPLAYWKQQLAGASQVLELRTDRARSASPTSSSNTIGFVLPPGV
                     TEAVKELSRSERTTLFMTFMAAFKVLLYRYTGQSDISVGTPYANRNLEEVQGLIGIFI
                     NVLVMRTDMSGNPGFRELLARVKKVAVDAYANQDVPFEKLVEVLKPDRNLSQTPLFQV
                     MFAFHNWDQESLKLNELNERSIRRESAATTKYDLSLEMMEHEGSIRGMLQYKSELFDE
                     STITRMVEHFQRLVENAVFKPEQPISTLRMMSPQEQQLLLEQWDNTKGHDQTTKCLDE
                     LFEEQVARTPEATAVVLGEEQINYRQLNERANQLARRLREQGVRPEVIVGILLERSVE
                     MIVAIFGVLKAGGAYLPLDRNLPVERVAWMMEDAGVEVLLTQESLKDWLPQRGRELRY
                     VLVEQIHNSEGAQVEERVESGVTGDNLAYVIYTSGSTGIPKGVCISHIAAANHFLVAL
                     EEYRIDSTDVVLVFASFNFDASVEEIFTGLLTGAALVLRGDPWGREDFFQCVADSGLT
                     IVNFPPAYWTQIVPAPGSETAALLAARLKLITIGGDQMPPQTARLWQQSGLQDLRLLN
                     GYGPTETVVTATLFDVPPLTEEDAASSQVPIGRPLAHRAIYILDQQGAPVPVGVTGEL
                     HLGGSLLARGYLNRPDLTAEKFIPDPFIGEAGARMYRTGDKATYLEDGQLRFLGRVDN
                     QVKIRGYRIELGEIEAGLYACDGVREAAVMVNERVAGEKRLIGYVVMEPGQTVVPGKI
                     KEHLRKRLPDYMVPTSFVELEKMPLTTNGKIDRRALPAPDEAARNIDLQYVEPKGPTE
                     EIIAGIWSSVLAIGRVGAEDNFFDLGGHSLLATQVISRVREALGVEVPLRTLFEQPTV
                     RGLAATVAGHLRAGERVRSLPLVPVGRDRALPLSFAQQRLWFLDQLEPQSSTYNMPAA
                     LRLTGALDRVALERSLSEVVRRHEALRTTFAETDGEPTQIIQPAQAIAVPLFDLSHLP
                     ETEREQQAQQLVAEEAARPFDLAAGPLLRAALIRLSGEEHIALLTMHHIISDGWSMGV
                     FIREMGALYEAYQKQEESPLSELAIQYADYAVWQREWLQGEVLDEQLSYWREQLGGAP
                     ALLELPTDRPRTKEQSHRGAHHGFTLEKAVADGL"
     gene            complement(4132..5505)
                     /locus_tag="VGB76_01350"
     CDS             complement(4132..5505)
                     /locus_tag="VGB76_01350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0097572.1"
                     /translation="MRILLAQPLGYLYATGGAHKANRMLMEGLAKRGHHCRALTAYRS
                     AESVQTREQFLSSLAKRGVSLSSSSPEADVFSLEGVEVHVSRDYLHQCSRLSDHVREF
                     KPDWVLVSEDTASVMLGAALEAAPARVVYIAHSPSTLPFGLDSFAADPDKTILLNKCA
                     GIITVSNYMQQYIERWSGIASAAIKFPVYGTEPFPRFGSFESGFVTMVNPCVIKGQTI
                     FLELARRFPDVEFAAVPTWGALNKDLELIKDLPNVRIIQPSERVDDILSQTRILLAPS
                     LWGEAYGQIVTEAMLRAIPVLASDSGGLLEAKLGTDYILPVRCIEGYEPVLDELKRTV
                     YVPLVPEQDVDPWEAALREVLTERATYERVSEQSREAATHFVNGLGFRPFEQYLEGLA
                     TEREKPEGQETDASKDGEFAGAMSEMSVEKRALIARRLKGRLEEVLNKRRTSSENSFD
                     RSGSKVS"
     gene            complement(5630..7096)
                     /locus_tag="VGB76_01355"
     CDS             complement(5630..7096)
                     /locus_tag="VGB76_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0097573.1"
                     /translation="MANIVVVTKGTSGDLYPFIRIGQALKARGHRVVLVTHWYGERHV
                     LRAGLDFAALDNPEEVPLYEEQTTLDAEREMNLVADEAVLVGSHAAGATAAETAAGSE
                     QPTTPSLSIFGMPLSRGVLEYEGISEQCRANDSILVTNYTAFFSARHVADRLGTPLVA
                     AFIAPSMFPQWVLDLSFFDELYAHVGRDLNRIRAKVGLPPVRDWRAWLTAADALVGLW
                     PEWFGAQQSGNGLKIVPVGFPCDVGTTGELPVELGDILDSGATPPLLITHGTSIPAKS
                     DFFTSAVEACRLSDMPALLVCKHEKLIPQPLPPQVKWFKFLPFDILMPHMRAIIHHGG
                     IGTCGQALAASLPQVVLALGYDRADNAARLQSLGVAEALTPLQWRPDALAGALQRLLN
                     APDIKERCSVYAQRMQHSDFAASAADVVEALAADVTRRKRAVEILPEAQDAASFPPPE
                     QRREPDDLHALVDSLSPARRALLKQRLKQKLSTPAQSK"
     gene            complement(7192..9873)
                     /locus_tag="VGB76_01360"
     CDS             complement(7192..9873)
                     /locus_tag="VGB76_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0097574.1"
                     /translation="MSEAKSKITTFDRELKAEQEYWIGRLPAEREESSLRLDYERPAS
                     YAADREQVEIVVPAELTQALAKLTGGSPFLTYTALMTVLKVCLYKYSENDLVIVGSPA
                     FQPEEVPGESANVLAILDEVSGRMSFKELLSSVRTSLREAYDRQNYPFERLLRDLKIE
                     RQENRSPLFDVALALTNIHTHFPSSELKNDITITFTSTPEALSGTFEYGRELFRRESI
                     ERLSEHFLNLLRRALGNMNARLAELEMLSDAERHKLLFEWNDTTTAFSHDRCIHQLFE
                     ARVEETPEAVALLFDAQTMTYAELNRRANQLAHSLQGRGIAPEVRVGILMDRSLEMVV
                     AILGVLKAGGAYVPLDPAYPKDRLAFMLHDSDAKVLLTQQRQEERLPEFQGEVFYLDA
                     GWETIAGESAEEPDSHATPENLAYIIYTSGSTGRPKGVQVEHRSLCNLAEAQSRSFGV
                     QPESHVIQLASFSFDASVSEIFMALVKGATLCLGYSEAVFSATAFVQLVREQEVTVAT
                     IPPAVLAVLPTEELTSLTTIIAAGEKCPAEVVARWSAGRRFFNAYGPTETTVCATLAE
                     CSPEKGALPPIGRPIDNAQVYLLDKDLQCVPIGAVGEICVGGVCLGRGYLNRPELTAE
                     SFVPNRFSAEPGARLYRTGDSARYRPDGQLEYLGRQDRQVKIRGYRIEIGEVEAALNG
                     LPKVRESAVIVREDVPGEKRLVAYVVGDTTEPDEAAEIREALRQRLPDYMMPSSVVLL
                     ESMPLTSNGKTDRRALAATDRERTSREEFVAPRNSVERKLAEIWSELLGIEQVDVNDS
                     FVVSIHDHFFDLGGHSLMAIKMFSRVREAFGVELPLNLIFTSAPTIAGLAQAIEQYML
                     ESADVNEVVAMLKELDELSDEEIKALLESEEEEAPQS"
     gene            complement(9958..11715)
                     /locus_tag="VGB76_01365"
     CDS             complement(9958..11715)
                     /locus_tag="VGB76_01365"
                     /inference="COORDINATES: protein motif:HMM:NF037824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0097575.1"
                     /translation="MSQESVYRLGKSAVVEPLVNRWAAWANLLPPVASSLHLQHYQLN
                     LLQSYLGDPMLHAQAGRDRKLRSGPFVDIPPERAGEVAELLERTRASMADNLELAQSL
                     MEFHTLLVREARGHSLEPYYRQVPPPLQGYVELIYDYYNRPIVRVFESLLYESKYYHS
                     ELQSLRLFRHTNDDARPFIMNTPRLPAEGQVDWEIPFASPQLDEFFKLDGVARPLGLI
                     RELLGLPADREKELLPLLTEEPSPAREEWNESSVRVRYIGHACVLVEWKGVAILTDPY
                     IGAVPTEGGLERFTYEDLPERIDYVLITHNHHDHFCLETLLRLRHRIGCVVVPKSSGV
                     FYGDISLKLLTQKVGFKNVVELEPLGSIPLPGGEIVAVPFLGEHADLPHSKSAFVVRA
                     GSEQILFGADSDCLDRQMYEHIRRILGPIQTIFLGMECVGAPLSWSGGAFLPVKPERS
                     HDQSRRYKGCDSSRAMEILEAVDAERIFIYAMGMEPWLEHLLGLGYTPDAVQILESNK
                     LLIDTRDRGFAVTERLYGKREIHFSQFPARATPAPRRRPAGENEQPPADAPVAASGER
                     APAKRRESLEQLEGEFIFD"
     gene            complement(11750..>13859)
                     /locus_tag="VGB76_01370"
     CDS             complement(11750..>13859)
                     /locus_tag="VGB76_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEY0097576.1"
                     /translation="ELTAERFIANPYRAGERLYRSGDRARHLPGGVIEYLGRKDEQIK
                     YHGYRVELGELRAALNTHPQIRDSVVAVRYDQHGREVMVGYYVARQELSGDELRAHMR
                     QRVYEEVMPSVWMRLRKLPLTLNGKVNYEELPGLAEARESVERVYEAPRTPSEEVVAG
                     IWGEVLGVQSVGAGDNFFELGGHSLLATQVISRVREAFGVDVPLRALFENRTVAGLGR
                     YIDSEIADAHHVSLPPILSVNRDAPLSLSFAQQRLWFLNQLEPDSVAYNIPAALRLEG
                     VLDISALERSLSEIVRRHEALRTTFVTTKDGEPIQIIQTAHAVELPLFDLSQLEPAER
                     EKRAQQLVAREAALPFDLARGPLLRAGLIRLAAEEHIALLTMHHIISDGWSLGVLVKE
                     VSVLYEAYSTGKESTLAELPIQYADYAAWQREWLQGEVLEEQLSYWRRQLRDAPSVLE
                     LPTDRPRPRVQSYRGAQEMFQLSGELSKELKALSRREGVTLFMTLLAAWKVLLHRYTG
                     QDRIPVGSPVANRERAETHDLIACFINTVVLHTDLSGNPDFQELMRRVREVTLEAYAH
                     QDLPFEVLVEALAPERKANYTPLFQVWFVFQNAPLQPMKLPGLTLEPLPNESQTAQFD
                     VALSMRESEQGIEGHMDYNTDLFNADTIIEMQKHFILILEAMVANPQTQLLDIPLGEE
                     NEYASINLQPVVQTSDEFVF"
BASE COUNT         2723 a         4250 c         3943 g         2943 t
ORIGIN      
        1 agaccatccg ccactgcctt ctcgagtgtg aagccgtggt gcgctccgcg atgactctgt
       61 tctttagtgc gtgggcggtc ggtgggcaac tccaacaggg cgggtgcgcc gcccagttgt
      121 tcgcgccagt agctcaactg ctcgtcgagc acttcacctt gcagccactc acgctgccac
      181 accgcataat ccgcatattg aatcgctaac tctgagagcg gcgactcttc ctgcttctga
      241 tacgcctcgt agagcgcgcc catctcacga atgaagactc ccatcgacca cccgtccgag
      301 atgatgtgat gcatcgtcag caaggcgatg tgctcttcgc ctgacaacct gatcagcgct
      361 gcccgcagca acggccccgc ggctaaatcg aaggggcgcg ccgcctcttc cgctacgagt
      421 tgctgcgcct gctgctcgcg ctccgtctca ggtaggtgcg agagatcaaa caacggcact
      481 gcgatggctt gcgccggttg aatgatttgc gtcggctcgc cgtccgtctc ggcaaatgtc
      541 gtgcgcaacg cttcgtgacg acgcacgacc tcagagaggc tgcgttccag cgccacccgg
      601 tcgagcgctc cggtcagtcg cagggcggca ggcatgttgt aagtgctcga ctgcggctcc
      661 agttggtcga ggaaccagag acgctgctgg gcgaatgaga ggggcaatgc cctgtcacgt
      721 cccaccggca ccagtggcag gctgcggact cgctctccgg ctctcaagtg tccggcgacg
      781 gtggcggcca gaccgcgcac ggtgggttgc tcgaagagcg tgcgcagcgg cacttccacc
      841 cctaaggctt ctctgacgcg cgagataacc tgtgtggcga gcaatgaatg tccgcccaag
      901 tcgaagaagt tatcttctgc gccgaccctg cctatcgcta agacgctcga ccagattccg
      961 gcgatgatct cttcggttgg accttttggc tcgacgtatt gaaggtctat gttgcgcgct
     1021 gcttcatcgg gtgcggggag ggcgcgacga tcaatcttgc cgttggtcgt caacggcatc
     1081 ttttccagtt cgacaaagct cgtagggacc atgtaatccg gcaaacgctt tcgtagatgc
     1141 tcctttatct tgcctggcac cactgtctgc cccggttcca tcaccacgta tccgattaga
     1201 cgcttctccc cggccacccg ctcgttaacc atgacggcgg cctcccgcac gccgtcgcag
     1261 gcgtacagcc ccgcttcaat ttctcccaac tcaattcgat acccgcgaat cttgacctga
     1321 ttatcaacgc gtccgaggaa gcggagttgt ccatcctcta aataggtcgc tttatcccct
     1381 gtgcgataca tgcgcgcccc tgcttcaccg atgaacgggt caggtataaa cttctccgcc
     1441 gtcagatcgg gacgattcag atagccgcgc gccagcaacg acccgccaag gtgtaactct
     1501 cccgtcacgc ccacaggcac gggtgcgccc tgctggtcga ggatgtagat ggcacgatgc
     1561 gccagcggac gcccgattgg tacctgcgat gaggcggcat cttcttccgt caacggcggc
     1621 acgtcgaaga gtgtggcggt cacgaccgtc tcggtggggc cataaccatt cagcaggcgc
     1681 aggtcttgca ggccggactg ttgccacagg cgcgccgtct gcggtggcat ctggtcgccg
     1741 ccgatggtga tgagtttcaa gcgcgcggct agcagggcgg ctgtctcgga gccgggcgcg
     1801 ggcacaattt gagtccagta agcgggaggg aagttgacta tggtgaggcc ggagtcggcg
     1861 acgcattgga agaaatcttc acgcccccac gggtcgccgc gcaggacgag cgcagcgccg
     1921 gtcagcaggc ctgtgaagat ctcttcgacc gaggcatcga agttgaaaga ggcgaacacg
     1981 aggacgacat cagtggagtc gatgcggtac tcttctaagg cgacgaggaa gtggttggcg
     2041 gcggcgatgt gagagatgca gacgcccttg ggaatgcctg tggagccgga agtgtaaatg
     2101 acatatgcga ggttatcccc cgtcaccccg ctctccacac gctcttcaac ctgcgcccct
     2161 tcgctattat gaatctgctc caccaacaca tatctcaact ctcttccgcg ctgcggcagc
     2221 cagtccttca gagactcctg tgtcagcaac acttctactc cggcgtcctc catcatccac
     2281 gccacacgct ccaccggtag attacggtcg aggggcaggt acgcgcctcc ggctttgagc
     2341 actccgaaga tggcgacgat catctcgacg gagcgttcca gcaggatgcc gacgataact
     2401 tccggcctga caccctgttc gcgcagtctt cgcgcaagtt gattggctcg ctcgttgagc
     2461 tggcgataat tgatctgttc ttcgccgagg acgaccgctg tggcctcggg cgtgcgtgcc
     2521 acctgctcct cgaagagttc gtccaaacat ttagttgtct gatcatgacc cttcgtgttg
     2581 tcccactgtt ccagcaaaag ttgctgctcc tgcggcgaca tcatccgcag agtggagatc
     2641 ggttgttcgg gtttgaaaac agcattttca accagcctct gaaaatgctc gaccatgcgc
     2701 gtgatcgtgc tctcatcgaa aagctcgctc ttgtattgca gcatgccgcg aatgcttccc
     2761 tcatgctcca tcatctcaag cgacaggtca tacttggtcg tcgccgcaga ctctctcctg
     2821 atgctccgct cgttgagttc gtttaacttc aacgactcct gatcccagtt atgaaaggca
     2881 aacatcacct gaaagagcgg cgtctggctc aggttacgat ccggcttgag cacctccacc
     2941 agtttttcaa acggcacatc ctgattcgca tatgcatcaa ccgcgacttt cttcacgcgt
     3001 gcgaggagtt cgcggaagcc ggggtttccg ctcatgtccg tgcgcatcac tagaacgttg
     3061 atgaagatgc cgatgagtcc ctgaacctct tcaaggttgc ggttggcgta tggcgtcccg
     3121 acgctgatgt ccgactgtcc cgtgtagcga tagagcagaa ccttgaacgc cgccatgaac
     3181 gtcatgaaga gcgtagttcg ttcactgcga ctcaactcct tcaccgcctc cgtcacaccc
     3241 ggcggaagca cgaagccgat tgtgttgctg ctagacgttg gcgacgcgga gcgcgcgcgg
     3301 tcggttcgta gctccaagac ctgcgaagcc cccgccagtt gttgcttcca gtaagccagc
     3361 gggccatcga gcgcgtcgcc cgataactgc gcgcgctgcc agagcgcgta gtccgcatat
     3421 tgaatcggca actcaggcag cgatgaggaa ttccccttcg agtaggcatc atagagcgtg
     3481 ctgacctcac gaatgaggat gctcattgac caaccatcgg aaacgacgtg gtgcattgtc
     3541 agcagcaaca ggtgctcctg ttcgtgcatc tttaccaaac ccacgcgcag catccggcca
     3601 ttcatgagat cgaaggggcg acgctcttct tcggagatca gttgttcggc acgcgcttcc
     3661 cgactcgctt catcaagctc gctcaggtct acgagcggca ggcgaagctc ttggggcggg
     3721 ttgatgcgct gtatgggcag gccgtccact tcgggaaagt tcgttcgcag aatttcgtgg
     3781 cggttgatca gttcgctgaa agttcgttcc atcccctgca cgttgagggg gcctgtaatt
     3841 cgtagtgcga cggcgatgtt atcttcgatg ctgtccggct ccatcttcca gatgaaccag
     3901 agacgctgct gtgcatagga caagggaatc gcctcgttat tttcgcggcg gggaatcttc
     3961 tgcactctgg aagcggcggc acctttcaac ttcaaacgca gcgcaagcag agcacgcttt
     4021 tcaggagaaa gattgtttaa ggactcaaga atattgctca ccaccggcct ccgttgtctg
     4081 tcttcaactg cgttttgcgc tcctactcag aaccgttcgg gcactatgtc ttcaactcac
     4141 ttttgaacct gatcgatcaa atgagttttc gctggaagtg cgacgtttat ttagcacctc
     4201 ttcgagccgt cctttgaggc gacgggcgat caaggcacgc ttctcaaccg acatctcact
     4261 catcgcgccc gcaaactctc cgtccttgct cgcatcagtc tcttgcccct ccggcttctc
     4321 acgttccgtc gccaaccctt cgaggtattg ctcgaacggc ctgaatccga gtccgttgac
     4381 gaaatgcgtc gccgcctcgc gcgattgctc cgacacgcgc tcataggtgg cgcgctccgt
     4441 aagcacttcg cgcagggctg cctcccaggg gtctacgtcc tgttcgggca cgagcggcac
     4501 atagacggtt cgcttcaact cgtcgagcac cggctcatat ccctcgatac aacgcacggg
     4561 caggatgtaa tcggtgccga gtttcgcttc gagcaggccg ccggagtcgc tggcgaggac
     4621 agggatcgca cgcagcatcg cttccgtgac gatctgaccg taggcttcgc cccagagcga
     4681 gggagcgagc aggatgcgtg tctgtgaaag aatgtcatca accctctcgc tcggctgaat
     4741 gatccgcaca ttgggcaggt ctttgatcag ttccaggtct ttattcagcg cgccccacgt
     4801 cggcacggcg gcaaactcca cgtccggaaa ccttctcgct aattccaaaa agatcgtctg
     4861 gcctttaatg acgcagggat tgaccatcgt gacgaagcca ctttcaaagc tgccgaaacg
     4921 cggaaacggt tccgtcccgt agaccgggaa cttgatcgcc gccgaggcga tgccgctcca
     4981 gcgttcgatg tattgctgca tgtagttgct gacggtgatg atcccggcac acttgttcag
     5041 caatatcgtc ttgtcgggat cggcagcgaa actgtcgagt ccaaacggca gcgtcgaagg
     5101 gctgtgagcg atgtagacga cgcgcgcggg cgcggcttcc agcgcggctc caagcatgac
     5161 ggaggccgtg tcttccgaga ctaataccca gtccggcttg aactctctga cgtggtcgga
     5221 caggcgcgag cattggtgca ggtagtctct ggagacatgc acctcgacgc cttcaaggct
     5281 gaacacgtct gcttccggcg aagaggatga gaggctgacg ccccgttttg cgagtgaact
     5341 caaaaactgt tcacgcgtct gcacgctctc ggcagagcga taggctgtca gggcgcggca
     5401 gtggtggcct ctcttcgcca gcccttccat cagcatccgg ttagctttgt gcgcgccgcc
     5461 cgtggcgtat agataaccca gaggttgcgc taataagatt ctcattcagg ttttgccttg
     5521 ctgttatgtc gcgtcttgta gaggcttctt ttgaaatgtt cgccggacac cggcggccaa
     5581 cgaccgcgat gcatctacgc gccatatcaa gatgaagatt gaggttgctc tatttgcttt
     5641 gagcgggtgt gctgagcttc tgctttaatc gctgcttgag gagagcgcgc ctggccgggg
     5701 agaggctgtc aaccagtgcg tgcagatcgt ccggctctcg tctctgttcg ggagggggga
     5761 acgaggcggc gtcctgcgcc tcaggcagta tttcaaccgc gcgcttcctg cgtgtcacgt
     5821 ctgcggctaa tgcttcgacc acgtcggcgg ctgaggcggc gaagtcgcta tgctgcatcc
     5881 gttgtgcata gacagagcaa cgctccttga tgtcgggggc gttcagcaaa cgttgcaacg
     5941 ctccggcaag cgcatcggga cgccattgca gcggcgtcaa agcctcggcc acgccgagcg
     6001 actggaggcg cgccgcgtta tccgcgcggt cgtagccgag cgccagcacc acttgcggga
     6061 gagaggcggc caacgcctgt ccgcaggtgc cgatgccgcc gtgatgaata atcgctcgca
     6121 tgtgcggcat gagtatgtcg aagggaagaa acttgaacca cttcacctgc ggcggcaaag
     6181 gctgcgggat caacttctca tgcttgcaga cgaggagagc cggcatgtcc gagagacggc
     6241 aggcttctac ggcggaagta aaaaagtctg acttcgcagg gatactggtg ccgtgtgtga
     6301 tgagcagcgg cggcgtcgcg ccggagtcga ggatgtctcc caactcgaca ggcaactcgc
     6361 ctgtcgtgcc gacatcgcaa gggaaaccca cgggcacgat cttcaagccg ttgccgcttt
     6421 gttgcgcgcc gaaccattca ggccacagcc ccaccagcgc gtcggctgcc gtgagccacg
     6481 cgcgccaatc cctgaccggg ggcagcccca ctttcgcgcg aatccggttg aggtcgcgcc
     6541 cgacgtgcgc gtagagttca tcgaagaacg acaagtcgag aacccactgc gggaacatgc
     6601 tcggcgcgat gaaggccgcg acgagggggg tgccgagtcg atccgcgaca tgacgcgcag
     6661 agaagaacgc tgtgtagttg gtaaccagaa tcgaatcgtt cgcacgacat tgttcgctga
     6721 tgccctcgta ctcaaggacg cctctgctga gtggcattcc gaatatcgag aggctcggcg
     6781 tggtcggctg ttcactcccg gcagccgtct cagccgccgt agccccggca gcatggctgc
     6841 caaccagcac cgcttcgtcg gccaccaggt tcatctcgcg ctcggcgtcg agcgtcgtct
     6901 gctcctcgta gagcgggact tcttcggggt tgtccagcgc agcgaagtcg agtccggctc
     6961 tcaagacgtg tcgctcgccg taccagtggg tcacgaggac gaccctgtgg ccgcgcgcct
     7021 tgagcgcctg accgatgcgt ataaaggggt acaaatcccc gctcgtcccc ttggtgacta
     7081 ctactatatt cgccatgatc taaccccgtt ttaaccgccg cccccgcgcg ctttgccctt
     7141 gctccggcgt tcgccagacc gccgcgtcgt ttaaaagtct ttcttaaata atcaactctg
     7201 gggagcctct tcctcttcgc tttcaagcag cgctttaatc tcttcatcgg agagttcgtc
     7261 caactctttc agcatggcga cgacctcatt cacatccgcc gactccaaca tgtactgctc
     7321 gatggcctga gccagcccgg cgatggtcgg ggcgctggtg aagatcaggt tcagaggcag
     7381 ttcgacgccg aaggcttcgc gcacgcgtga gaacattttg atcgccatca gcgagtggcc
     7441 gccgaggtcg aagaagtggt cgtgaatgct gaccacgaaa ctgtcgttca catccacctg
     7501 ctcgattccg agcagttccg accagatctc ggcgagcttt ctctcgaccg agttgcgcgg
     7561 ggcgacgaac tcctcccggc tcgtgcgttc gcggtcagtt gcggcaaggg cgcgtcggtc
     7621 tgttttgccg ttggaggtca gaggcatcga ctcaagcaga acgacggagg acggcatcat
     7681 gtagtctggg agtctctgcc tcagggcttc acgtatctca gcggcctcat caggctccgt
     7741 cgtgtcgccc accacgtagg ccacgagccg tttctcgccg ggcacatcct cccgcacgat
     7801 gaccgcgctt tcacgaactt tcggaagccc gtttaacgcg gcttcaactt ctccgatctc
     7861 gatgcggtag ccgcgaatct tgacctgccg gtcttgccga ccgagatatt cgagttggcc
     7921 gtcggggcga taacgcgcgc tgtcgcccgt ccggtagagt cgcgctccgg gttccgcgct
     7981 gaaacggttg ggcacgaatg attcagccgt cagttcggga cgattgagat agccgcgtcc
     8041 gagacagaca ccgccgacgc aaatttcgcc caccgcgcca atcggcacgc attgcaggtc
     8101 tttatcaagc agataaacct gcgcgttatc aatcgggcga ccgatgggag gcagcgcgcc
     8161 cttctccggc gaacactcgg ccagagtggc acacacggta gtttcagtcg gcccgtaggc
     8221 gttgaagaaa cgtctgccgg cagaccagcg cgccacaacc tccgcagggc atttctcccc
     8281 ggcggcaatg atcgtcgtga gcgaggtcaa ctcttccgtc ggcaggacag ccagcacggc
     8341 gggcgggatg gtggcgaccg tcacctcctg ctcacgcacc agttgcacaa atgctgtcgc
     8401 ggagaagacg gcttcgctgt aaccgaggca gagcgtcgct cccttgacga gcgccataaa
     8461 gatttccgac accgaagcgt cgaagctgaa agaggcaagc tggatcacat ggctctccgg
     8521 ctgcacaccg aaactgcggc tctgcgcttc ggccagatta cacagactcc tgtgctcgac
     8581 ctgcacgccc tttggtcgcc cggtcgaacc tgaggtgtag atgatgtagg cgagattttc
     8641 aggtgtagcg tggctgtcag gctcctccgc gctctccccc gcaatcgtct cccagcccgc
     8701 gtccagatag aagacctcgc cttgaaactc cggcagtcgc tcttcctgtc tttgctgtgt
     8761 gagaagcact tttgcgtccg agtcgtgcaa cataaaagcg aggcggtctt tcggataggc
     8821 gggatcgagc gggacatacg cgccgcccgc cttgagcacg ccgagtattg ccaccaccat
     8881 ctccagagag cggtccatca ggatgccgac gcgaacttcc ggagcgattc cccgcccttg
     8941 caaagaatgc gcgagttgat tagccctgcg attcaactcg gcgtaagtca ttgtctgcgc
     9001 gtcgaagagg agcgcgacgg cctccggcgt ctcttcgacg cgagcctcga acaattgatg
     9061 aatgcaacgg tcgtgcgaga aagctgtggt cgtgtcgttc cactcaaaca gcagcttgtg
     9121 ccgctcggcg tccgacagca tctccagttc ggcaaggcgc gcgttcatgt ttcccagcgc
     9181 acgacgcagg aggttcagga agtgctcgct caggcgctct atgctttcac gccggaacag
     9241 ttcgcggcca tattcaaacg tgccggacag tgcttcgggc gtagaggtaa aggtgatcgt
     9301 gatgtcgttc ttcaattcgg atgaaggaaa gtgcgtgtga atgttcgtca acgcgagcgc
     9361 cacgtcgaag agcggcgacc tgttctcctg tcgctcgatc ttcaaatctc tgagcagccg
     9421 ctcgaatgga tagttctgcc tgtcgtaagc ttcgcgcagg ctcgtgcgaa cgctcgacaa
     9481 cagttccttg aaactcatgc ggccactcac ctcgtcgaga atcgccagca cgttcgcgct
     9541 ctcgcccggc acctcttccg gctgaaaggc ggggctgccc acgatgacga gatcattttc
     9601 ggaatacttg tagagacaga ctttcagcac ggtcatcagc gcggtgtaag tcaagaaggg
     9661 cgagccgcct gtgagtttcg ccaacgcctg cgtcagttct gccgggacga ctatttcaac
     9721 ctgttccctg tccgccgcat aactcgccgg tctttcataa tctaatctga gactggactc
     9781 ttcccgttca gccgggagac gaccgatcca gtattcctgt tccgccttta actcccggtc
     9841 aaaggttgtg attttcgatt tagcttcgct catcgtgtta actcctccgc ttgtaatctg
     9901 gaaaggcggc gggtgtcggt tgctcgtcgc ccctgcatct attcgtcgcc tcgataacta
     9961 atcaaagatg aattcgcctt ccaactgttc caggctctcg cgccgctttg ccggagcacg
    10021 ctccccggac gcagccacgg gcgcatccgc cggaggctgc tcgttctccc cggctggccg
    10081 tctgcgcggc gcgggtgtcg ctctcgcggg gaattgtgag aagtgaatct cgcgttttcc
    10141 atagaggcgt tcggtgacgg cgaagcctct gtcgcgtgta tcaatcaaga gcttgttgga
    10201 ttccagaatc tgcacggcat ccggcgtgta gcccaatccc agcaggtgtt cgagccacgg
    10261 ctccatgccc atcgcataga tgaaaattcg ttcggcgtcc acggcttcga gaatctccat
    10321 cgcgcgactc gaatcacaac ctttatacct gcgcgactgg tcgtggctgc gttccggctt
    10381 aacgggcaaa aacgctcctc cgctccatga gagcggcgcg ccgacacatt ccatgccgag
    10441 aaagatggtt tgaatgggac cgagtatgcg ccttatgtgc tcgtacattt gccggtcgag
    10501 acaatcggaa tccgccccga agaggatctg ctcgcttccg gcacgcacga cgaaggccga
    10561 cttggaatgt ggcaggtcgg cgtgctctcc caaaaaagga accgccacga tctcgcctcc
    10621 cggcagcggg attgaaccca acggttccaa ctccacgacg ttcttgaagc ccactttttg
    10681 cgtcagaagt ttaagtgaga tgtcgccgta gaagacgccc gacgatttcg gcaccaccac
    10741 acagccgatc cggtggcgca ggcgcagaag cgtctctaga caaaagtggt cgtgatgatt
    10801 atgtgtgatc aggacgtaat cgattctctc cggcaggtct tcataagtga accgctcaag
    10861 gccgccctcg gtcggcaccg cgccgatgta ggggtcggtc aggatcgcta cgcccttcca
    10921 ctcgaccagc acgcaggcgt gcccgatata acgcacgcgg acgctcgact cgttccactc
    10981 ttcgcgcgcg ggcgaaggct cttccgtgag cagaggcaac agttcctttt ctctgtccgc
    11041 aggcagcccc agcaattcac gtatcaggcc gagggggcgc gcaacgccgt cgagtttgaa
    11101 gaattcatca agctgcgggc tggcaaaagg tatctcccaa tccacctgac cctccgccgg
    11161 gaggcgcggg gtattcatga tgaacgggcg tgcgtcgtcg ttcgtgtgcc ggaagagtct
    11221 gagcgattga agttcgctgt gatagtactt gctttcataa agaagacttt cgaagacgcg
    11281 cacgatggga cggttatagt agtcgtagat caactcgacg tagccctgca aaggaggcgg
    11341 cacttgcctg tagtacggtt cgaggctgtg ccctctggcc tctctcacga gtaaggtatg
    11401 aaactccatc aggctctgtg ccagttcgag attgtcggcc atgctggcgc gcgtgcgctc
    11461 caataactcg gccacctccc ctgctctctc cggcggaata tccacaaagg gtccgctgcg
    11521 aagtttccga tcccggcctg cctgtgcatg taacatcgga tcgcccagat acgactggag
    11581 gaggttgagt tgataatgtt gcaggtggag gctggatgcc acgggcggca acagattagc
    11641 ccacgccgcc caacgattaa ccagtggttc aactacggcg gactttccta gccggtacac
    11701 ggactcttgc gacatggctt tggctcctta cctattccct ctgaccttgc tagaagacga
    11761 attcatcaga cgtttgaacg acgggttgca gattgatgga ggcatattca ttctcttcgc
    11821 cgagcggtat atccagcagt tgcgtctgag gattggcaac catagcctcc agaatgagga
    11881 tgaagtgctt ctgcatctcg atgatggtgt cggcgttaaa gagatcggtg ttgtagtcca
    11941 tgtgcccttc aatgccctgc tccgactctc gcattgatag cgcaacatcg aactgcgccg
    12001 tctggctttc gttgggcagc ggctccaaag tcagaccggg caacttcatt ggttgcaacg
    12061 gcgcgttctg gaagacgaac cagacctgaa acagcggcgt ataattcgcc ttccgctcag
    12121 gcgctaaggc ttccaccagc acttcgaagg ggaggtcttg gtgcgcatag gcttcgagtg
    12181 tcacctcacg cacgcgtctc attaactctt gaaaatcagg gttgccggac aggtctgtat
    12241 gcagcaccac cgtattgatg aaacacgcta tcaagtcgtg tgtctccgcg cgctcccggt
    12301 tcgcgacggg tgagccgacc ggaattctgt cctgtccggt gtagcgatgg agcaacacct
    12361 tccacgcggc cagcagcgtc ataaacagcg tcaccccttc gcgccggctt aaagccttta
    12421 gttctttcga caactcgccg ctcaactgaa acatctcctg cgcgccgcga taggactgca
    12481 cgcgcgggcg cgggcggtcg gtgggcaact ccagcacaga gggcgcatcc cgcaactgcc
    12541 tgcgccagta actcaactgc tcttcgagca cttcgccttg caaccactcg cgctgccatg
    12601 cagcataatc cgcgtattga atcggcaact ccgcaagcgt tgattccttg ccggtgctgt
    12661 aagcctcgta aagcacactt acctccttca ccagcacacc cagcgaccac ccatcactga
    12721 taatgtggtg catcgtcaac agggcgatgt gctcttctgc cgccagccgg atcaatcccg
    12781 cgcgcagcaa tggccccctc gccaaatcga acgggagtgc ggcttctcta gccacgagtt
    12841 gctgtgcccg cttctcgcgc tccgcaggct caagctgaga gagatcaaag agcggcaact
    12901 cgacagcatg cgccgtttga atgatctgaa tcggctcccc gtctttcgtc gtaacaaacg
    12961 tcgtgcgtaa cgcctcgtga cggcgcacta tctcggacag gctgcgctcc agcgcagaaa
    13021 tgtcgagcac gccctccaga cgtagggcag cagggatgtt ataggcaacc gagtctggtt
    13081 caagctggtt aagaaaccac agtcgctgtt gcgcgaacga gagggagagc ggcgcatctc
    13141 tattgacgga cagaatcggc gggagtgaga cgtgatgcgc atcagcgatc tctgagtcaa
    13201 tatatctgcc cagccccgca acagtgcgat tctcaaacag agcgcgcagc ggcacatcaa
    13261 cgccaaacgc ctcacgtaca cgcgagatga cctgtgtagc caacaatgaa tgcccgccca
    13321 actcgaagaa gttatctccc gcgcccacac tctgcactcc gagcacctcg ccccagatgc
    13381 ccgccaccac ctcctcgctc ggcgtgcgcg gcgcttcgta cacgcgctca accgactcgc
    13441 gcgcctcagc cagtcccggc aactcctcgt agttgacctt cccgttcaat gtcagcggca
    13501 actttcgcaa ccgcatccag acgctcggca tcacctcttc atagacgcgc tgacgcatgt
    13561 gcgcacgcaa ctcatctccg cttagttcct gacgagccac gtaatagccg accattactt
    13621 cgcggccatg ctggtcgtat cgcaccgcca ccacgctgtc tctaatctgc gggtgtgtgt
    13681 taagtgctgc acgcaactca cctaactcca cccgataccc gtgatacttg atctgctcat
    13741 ctttacgtcc taagtattcg atgacgccgc ccggcaaatg gcgtgcgcgg tcgccgctgc
    13801 gatagaggcg ttcgcctgcg cgataaggat tggcaatgaa gcgttcagcc gtcagttcc
//
LOCUS       DASWNS010000040        11233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_468901, whole genome shotgun sequence.
ACCESSION   DASWNS010000040 DASWNS010000000
VERSION     DASWNS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468901"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VGB76_01375"
     CDS             <1..442
                     /locus_tag="VGB76_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0097577.1"
                     /translation="DPLSVHNDRSVAEAYSNRGFIRTNKLDMPGALFDFNKALECYQG
                     NPDLYFKRGRARLINGDLGGAITDFTDGLALKPESSLASLAYASRGYAYMLQGNEAEA
                     RKDFDQSSKVNKEGKIFLHLHLMTLDEQLKEMKRLRTRDQQRET"
     gene            complement(537..1784)
                     /locus_tag="VGB76_01380"
     CDS             complement(537..1784)
                     /locus_tag="VGB76_01380"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEY0097578.1"
                     /translation="MNVLVLNCGSSSVKFQLIATDLERIAENTDRRLARGVVERIGGE
                     AIITLQAEGGTAQRSTAPLRDTRAAVESVIRWATSEASGIAEVRSVGDVHAVGHRVVH
                     GGERFTHSVLITEEVWRGIEDCIDLAPLHNPANLKGIAAAREVFGAGVPQAAVFDTAF
                     HQTIPDHAYLYALPYQLYRRYRVRRYGFHGTSHRYVAYRYRQLRNIERAATNLITLHL
                     GNGCSVAAIRAGDSVDTSMGMTPLEGLVMGTRSGDIDPAIIDYVAAKEGLSAQEVETL
                     LNKQSGLLGISGLTNDMRELLDEAHESDDRRARLAIEIFCYRARKYIGAYLAALNGAD
                     AIVFTGGIGENSAEIRARICDGLAWLGVELDTEKNALHHGGREGLISGDASRLAVYVI
                     PTDEELLIARDTVRCVHGAPQRY"
     gene            complement(1887..3287)
                     /locus_tag="VGB76_01385"
     CDS             complement(1887..3287)
                     /locus_tag="VGB76_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0097579.1"
                     /translation="MNSLDSTENNSQPVKLYCARWLLPITSPTIEDGAVAVAGKLIVG
                     VGSRAEVESQFPEASREDFGEAAIIPGLVNCHSHLELTAMRGYLEDVEGDFFAWLRKL
                     TVARMTRMTADDLRVSATWGAVEAARAGVTCLGDACSAGDASLSALRDVALRGIVYQE
                     VIHPDERAADEMLAKLKDDVVRLRERENELVRVGVSPHAPYTMSEKLLVGVADYAFAE
                     KLPLMIHAAESRYEDELLKHGSGVFAENYVRRAIEWRSPRMSPIQYLSTRGVLATRPL
                     LAHCVRVDAADIETIREARAAVAHCPKSNLKLGHGHAPFAKMSGVRRGFGSDSVASNN
                     TCDMLEEARFATLLARLHQTEEGVEIYNAHDALQDATRGGARALGYEENLGALNAGDE
                     ADLAIFKLDGAHQIPVYDPAHALIFSSSAHDAVLTLVAGREVYRDGKVLTVDEERLRA
                     RMAEIRRKVTGNKSDE"
     gene            complement(3284..4690)
                     /locus_tag="VGB76_01390"
     CDS             complement(3284..4690)
                     /locus_tag="VGB76_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0097580.1"
                     /translation="MTYTQLLKGNANFRHLWSGQVVSEIGDWLNNIAVLALALELAGA
                     GHQGRAIAIYAIARHLPLFLFGPIAGVVVDRADRRRVMIVADVLRAVLAAGFLLAQMF
                     SSLPTIYAVGASLFAVSAFFNAAKRATIPVIVTDADELLNANSLSASTTAATIAVGSA
                     LGGLVATFISRDIVFLLNAATFIVSALLISRIRLPHGRSAGSETDALEITAIETSRGR
                     ISASQGRVALSQGRVASARGRIVTGLRRAFVEFREGLHYVRSDGVLAAVFLVAAGWGL
                     GNGVARALYSVFGARLGELTASAWAARPGDFGISVLFVAMGAGGVLGAPVARRFNAAG
                     DSLGARMGRSLVLDGCGLALFTLMPNLWTASLVLIAREMNFAIWWTAQQTLIMRRTEN
                     AYAGRVFASYETLTTLMMVASMYAAGAGADAFGLRPVAALGGLTIAFSGLLWFLLRRR
                     HRHAANAASTTNAQHLST"
     gene            complement(4687..5289)
                     /locus_tag="VGB76_01395"
     CDS             complement(4687..5289)
                     /locus_tag="VGB76_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0097581.1"
                     /translation="MPVLKRLVLAALILSVLAAAGLYFFNQYWIHRFDPLIARQASVY
                     RLDPDLVWSIIYEETYFSPWKIGEDAEIGLMQVTPTVGREWAAETGMREFAQQMARDP
                     KSLLSDPERNIQVGCWYLEKISKDYRDQPDAEVRMVAAYNAGPSRAAEWNRAGAGQAP
                     LTAGQFIERIDIASTRAYVSSILARYRELKTAQRGGQPKK"
     gene            complement(5330..6118)
                     /locus_tag="VGB76_01400"
     CDS             complement(5330..6118)
                     /locus_tag="VGB76_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097582.1"
                     /translation="MEETFEAIGIDVPSERDFNHLAERAGDQGEPSQLLRRGAVLHGR
                     CWKLGEGLEVWTVLYESGTGDIFYADCRPGFRARYTQRISPWALTEYDEEGEAIVHGY
                     CDGTETEVLFELQNLTEVGFEGFNGQELHVGLCGLAYRARVCAREERPRWKALEEYAP
                     ARAAHENDWSLCGRVLSFKPLRNPLSGGELYWAYLDLEHFKLEVLINQRSLRGGALRV
                     GSTLAADVWLQGHVLDRRALLSRYEGLDRSIKQAELWTRLRRRN"
     gene            complement(6216..6443)
                     /locus_tag="VGB76_01405"
     CDS             complement(6216..6443)
                     /locus_tag="VGB76_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097583.1"
                     /translation="MYRKPRFLEELHAIREEMSREADYDVELFAEMTRTGARPSHGPE
                     RNIRGFKSRAPRAGEREEKLKSIQPRKRATR"
     gene            complement(6524..7000)
                     /locus_tag="VGB76_01410"
     CDS             complement(6524..7000)
                     /locus_tag="VGB76_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0097584.1"
                     /translation="MKQSLYLETSVVGAYLDNGEPFRRDLTIRWWEHEMSEYRAVISP
                     LVMRELERVPEPHRTAYLKLIAPLAQLELTDEATILAEGYISRGIFHRKYIGDALHVA
                     VASVHKIDYLVTWNFGHLANVRRQARIRLFNTAAGFFVPMIVTPEFLVSELTEKKD"
     gene            complement(6997..8748)
                     /locus_tag="VGB76_01415"
     CDS             complement(6997..8748)
                     /locus_tag="VGB76_01415"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEY0097585.1"
                     /translation="MKKLSRVLWDSLAVSLICVAMCGADARAFAWAGDKNLEKAARAI
                     RQGEFEEAEKLYRAAVERDPRDVPARLGLSFALLKQRNLRGAFEQAARVLALDPTSAR
                     AHALLGSALLASGDFKNSIEEFRTALVFKEDEALAIAGLSMINFYENRASVALTGLRR
                     AIYLDPKEPDYIYSYAQAAARSERYREAADAYEEFLHIAPRTDADRRARIRGLISFLR
                     YLGAQRNLYQTTGAPRTTFSFELSNSRPIVSVHVNGSKTPLRFVVDTGSGMCVLSTAT
                     AERLGIKPIAEGGMARAVGGGGRFAIVYGFLSSLQIDGARIENVPVYIRNFHNQQEAV
                     DGYIGLSVLSKYVTSVDYGTREMTLVRNADRDDAPRPAAPRAIDRLADNVFADNAAPP
                     PTVSTLLGTPTSRPTLKPAPSSASPGSDTSRSYEFPIRATSSGFWSSAVTLDGVEKPL
                     NFIVDTGASISVVSTQLAKTEDMSRFEQKTRLKVFGAAGVSEDVMTLLLPRVTLGDYT
                     HSNLSAAVLDMSAINETCGFEQTGIIGGNILRSFRVTFDFQRGLVRLDPLTTQPAPVT
                     SPPTRDVNITPASGATP"
     gene            complement(8867..9529)
                     /locus_tag="VGB76_01420"
     CDS             complement(8867..9529)
                     /locus_tag="VGB76_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097586.1"
                     /translation="MPRDKQPVTIEALRETHRARATDIRRRLAEFQDVWREGSDARLW
                     EELVFCIFTAGASARMGLRSVEAVRALLAEGAEPELAHALGGVHRYPNSRSGYIVVTR
                     DYLREDCGMRLRERLLSFDDPLARRDWLARERRIKGLGYKEASHYLRNIGFRGYAILD
                     KHILRSLAELGVLESPAPPTTRARYLVIEESLRRFAGRLAIDFDELDLVLWSMKTGEI
                     LK"
     gene            9829..11205
                     /locus_tag="VGB76_01425"
     CDS             9829..11205
                     /locus_tag="VGB76_01425"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY0097587.1"
                     /translation="MVGSKIRQLLFVSTLLLSCYASAAAQDDSTPAPQPSTSATPYVV
                     STWPETLPVRSSPDIPDLLAAQPAPTPVNYNQGVLVETIDGRVVLEQGANQQYNPASV
                     VKLATALDALQTFGPNYRFSTVVWTNGTFDQATGTITGDLIISGRDPSLHYEHAVAIA
                     RELNRLGIRTVTGDLYVPPKFTMNFNWSAQRSGDLFYDTLDSTRRPAAATRAWFDERI
                     AHGDQAATQTIPSVAVMGAVYVASVPQNARVLITHRSSKLVDVLKVLLCYSNNFMAER
                     LGDNLGGAAGLEHFLISKVGLAPGDVRLASTSGLGVNRLSPRSMMKIYRALLKELDKH
                     NLSPSDIMPVAGIDPGTLQKRYTFGYSRGSVIGKTGTLGRTDGGASALAGQMRTKTGE
                     TLLFVIFNRGGSVARFRETQDRLVSELQFTRGGPAPFAYSPQTLAMRLSDTEFEQADA
                     SEFEPKTN"
BASE COUNT         2212 a         3495 c         3435 g         2091 t
ORIGIN      
        1 cgatccgctc tccgtgcaca acgaccgcag cgtggcagaa gcctactcga atcgcggctt
       61 catacgcacc aacaaactcg acatgcccgg cgcgctgttc gatttcaaca aagccctcga
      121 atgttatcag ggcaatcccg atctctattt caaacgcggg cgcgcacgcc tgatcaacgg
      181 cgacctcggc ggcgcgatca ccgatttcac cgacggcctc gcactcaaac ccgaaagcag
      241 cctcgcctcg ctcgcctacg cctcacgcgg ctatgcctac atgctgcaag gcaacgaagc
      301 ggaggcgcgc aaagatttcg atcaaagctc aaaggtcaac aaggaaggca agatctttct
      361 ccacctccac ctcatgacgc tcgacgagca actcaaagag atgaaacgcc tgcgcacccg
      421 cgaccagcaa cgcgagacgt agaaaagata aacgatgaag taggaacgat gaacgcggaa
      481 ctgaaagcac ctgttttcag ttccgcgttc atcgttccta cttcatcgtt tcccgctcaa
      541 tatctttgcg gcgcgccgtg gacgcagcgc acggtgtcgc gtgcaatgag caattcttca
      601 tcggtgggga tcacatagac ggcgagccgt gaggcgtcgc cgctgatgag cccttcgcgc
      661 ccgccgtgat gcagcgcgtt cttctctgtg tcaagttcga cgccgagcca cgcgagtccg
      721 tcgcagatgc gtgcgcgaat ctccgccgaa ttttccccga tgccgcccgt gaagacgatg
      781 gcgtccgcgc cgttcaaagc cgcgagatac gcgccgatat atttgcgcgc gcggtagcag
      841 aagatttcga tggcgagccg cgcgcggcgg tcgtcggatt cgtgcgcttc gtccaacagt
      901 tcgcgcatgt cgttcgtcaa gccggagatt ccgagcagcc ccgactgctt gttcaagagc
      961 gtctccacct cctgcgccga cagaccctct ttcgccgcaa cgtaatcgat gatcgccgga
     1021 tcgatgtcgc cggagcgcgt gcccatcacc agaccttcga gcggcgtcat acccatcgaa
     1081 gtgtcaaccg agtcgcccgc gcgaatggcc gccaccgagc acccgttgcc gagatggagc
     1141 gtgatcaggt tggtcgccgc gcgctcgatg tttcgcagtt gccggtagcg ataggcgacg
     1201 tagcgatgcg acgtgccgtg gaaaccatag cgacgcaccc ggtagcgtct gtagagttga
     1261 taaggcaggg cgtagaggta agcgtgatcg gggatggtct ggtgaaacgc cgtgtcgaag
     1321 acggcggctt gcgggacgcc cgccccgaac acctcgcgcg ccgccgcgat gcctttgagg
     1381 ttcgcgggat tatgcagcgg cgcgaggtct atgcaatcct ctatgccgcg ccagacctct
     1441 tccgtgatca agacagaatg ggtgaaccgc tcgccgccgt gcacgacgcg atgaccgacc
     1501 gcatgcacgt cgcccacgct gcgcacctcc gcgatcccgg aggcttccga cgtagcccaa
     1561 cggataacag attcaaccgc cgcacgcgta tccctgagcg gcgcggtcga acgctgtgcc
     1621 gtgccgcctt cggcctgcaa agtgatgatc gcttcgcctc cgatgcgttc caccacgccg
     1681 cgcgccagac gccgatccgt attctccgcg atccgctcaa ggtcggtcgc tatcagttga
     1741 aacttcacgg acgaactgcc gcagttgagc acaagtacat tcataataat tcagtgacgg
     1801 gtaacgggaa gaaaagtgat gagtaatgag taatgagtga tgagtaaaac ccaaagagtt
     1861 gtctttcact catcactcat tactcatcac tcatcacttt tatttcccgt cactttcctt
     1921 ctaatttccg ccatgcgcgc gcgcaggcgt tcttcgtcaa cggttaagac tttgccgtct
     1981 cgataaactt ccctgcccgc gacgagcgtg agcacggcgt cgtgcgccga agaggagaag
     2041 atgagcgcgt gcgccggatc gtagacgggg atttggtgcg cgccgtcgag tttgaagatg
     2101 gcgaggtcgg cctcgtcgcc tgcgttgagc gcgcccagat tctcttcgta gccgagagcg
     2161 cgtgcgccgc cgcgcgtggc atcctgcaaa gcatcgtgcg cgttgtagat ttcgacgccc
     2221 tcttccgtct gatgcagacg cgcgagcagc gtggcgaagc gcgcttcttc gagcatgtca
     2281 caagtgttgt tgctggcaac cgagtcgctg ccgaagccgc gccggactcc agacatcttc
     2341 gcaaacggcg cgtggccgtg gccgagtttg aggttcgact tcggacagtg cgcgaccgcc
     2401 gcgcgggctt ccctgatcgt ttcgatgtcc gccgcgtcaa cgcggacgca atgcgcgagc
     2461 agaggccgtg tcgcaagcac gccgcgcgtc gagagatatt gaatcggcga catgcgcggc
     2521 gaacgccatt cgatggcgcg gcgcacatag ttctccgcaa acacgccgct cccgtgtttc
     2581 aacaactcgt cttcgtatcg agactccgcc gcgtgaatca tcaacggcaa cttttccgcg
     2641 aatgcataat cggctactcc caccagcaac ttttcgctca tcgtgtacgg cgcgtgcggc
     2701 gagacgccga cccgtacgag ttcgttctcc cgctcgcgca gtcgcacgac atcgtctttc
     2761 agcttcgcca acatttcgtc tgcggcgcgt tcgtccgggt ggatcacttc ctgatagacg
     2821 atgccgcgca aggccacatc gcggagcgca gacaggctgg cgtcgcccgc gctgcacgca
     2881 tcgcccagac acgttacgcc cgcacgcgcc gcttccaccg cgccccacgt cgcagacacg
     2941 cgcaagtcgt cagccgtcat acgcgtcatg cgcgcgaccg tcagtttgcg gagccacgcg
     3001 aagaagtcgc cttctacgtc ttccagatag ccgcgcattg cggtcagttc cagatgcgaa
     3061 tggcaattga ccagaccggg aatgatcgcc gcttcgccga aatcttcgcg cgacgcttcg
     3121 ggaaactgcg actcaacttc agcccggctc ccgacgccga cgatcaactt ccccgccacc
     3181 gcgaccgcgc cgtcttcaat cgtcggcgac gtgatgggga gcagccagcg cgcgcagtaa
     3241 agtttcaccg gctgagagtt gttttcggtt gagtcgagag agttcatgtc gagagatgtt
     3301 gcgcgtttgt cgtcgaggcg gcgttggccg cgtgcctgtg ccttcgtctg agtagaaacc
     3361 acaggaggcc ggaaaaggca atcgttaaac cgccgagcgc ggccacgggg cgaaggccga
     3421 aagcgtctgc gcccgcgccc gccgcgtaca tcgaagcgac catcatcagc gtcgtcaaag
     3481 tttcgtagga ggcaaagacg cgccctgcgt aagcgttctc cgtgcgacgc atgatgagcg
     3541 tctgttgcgc cgtccaccag atggcgaagt tcatctcgcg cgcgatgagc acgaggctcg
     3601 ccgtccacag gttgggcatc agagtgaaaa gcgcgaggcc gcagccgtcg agcacgagcg
     3661 aacgtcccat gcgtgcgccc agactatcgc ccgccgcgtt gaagcgacgc gcgacgggcg
     3721 cgccgagcac gccgcccgcc cccatcgcca cgaacaacac agagatgccg aagtctcccg
     3781 gtcgcgccgc ccacgccgat gccgtcaact cgccgagccg cgcgccgaac acgctgtaga
     3841 gcgcacgcgc cacgccgttg cccaaccccc aaccggcagc caccaagaag accgccgcca
     3901 gcaccccgtc gcttctcaca taatgcaaac cttctcgaaa ctctacgaac gcgcgacgca
     3961 aacccgtcac gatgcgacca cgcgccgaag cgacacgccc ctgcgacaaa gcgacgcgcc
     4021 cctgcgacgc actgatgcga ccgcgcgacg tttcaatagc agttatctca agcgcatcgg
     4081 tttcgctccc cgcgcttcgc ccgtgcggga gacgtatgcg gctgatcaac agggcggaga
     4141 cgatgaaggt ggcggcgttg agcaggaaga ctatatcgcg gctgatgaag gtggcgacga
     4201 gtccgccgag cgcggagccg acggcaatgg tcgcggcggt ggtcgaagcc gagagcgagt
     4261 ttgcgttgag cagttcgtcc gcgtcagtga cgatgacggg aatcgtggcg cgtttcgcgg
     4321 cgttgaaaaa ggccgagacg gcgaagagcg acgcgccgac agcatagatc gtcggaagcg
     4381 acgaaaacat ctgtgcgagc aaaaatccgg cggccagcac ggcgcgcaaa acgtctgcca
     4441 caatcatcac gcgcctgcgg tcggcgcgat ccacgacgac gcccgcgatg ggtccgaaga
     4501 ggaagagagg caggtggcgc gcgatggcgt agatggcgat ggcgcgacct tgatgcccgg
     4561 ctccggcgag ttcgagcgcg agcgcgagca cggcgatgtt gttgagccag tcgccgatct
     4621 cggagacgac ctgccccgac cagagatggc gaaagttcgc gttccccttg agcagttgcg
     4681 tgtaggtcac ttcttcggct gcccgccgcg ctgcgctgtt ttcagttcac ggtaacgcgc
     4741 gaggatcgat gagacgtaag cgcgcgtgga agcgatgtca atgcgttcga taaactgccc
     4801 ggcggtgagc ggcgcttgtc ctgcgcccgc gcgattccac tcggcggcgc ggcttggtcc
     4861 ggcgttgtaa gcggcgacca tgcgcacctc cgcgtcgggc tggtcgcgat agtctttcga
     4921 gattttttcc aggtaccagc agccgacctg tatgttgcgt tccgggtcgg agagcaggct
     4981 tttggggtcg cgcgccatct gctgcgcaaa ctcgcgcatc cccgtctccg cagcccactc
     5041 gcgcccgacc gtcggcgtca cctgcatcag accgatctcc gcgtcctcac cgatcttcca
     5101 agggctgaaa tacgtctcct cgtagatgat gctccagacg agatcggggt cgagccggta
     5161 gacgctcgcc tgccgcgcga tcaaggggtc gaaacgatga atccaatatt gattgaaaaa
     5221 atagaggccg gcggcggcga ggacggagag gataagggct gcgagaacga gacgcttgag
     5281 aactggcatt caactgaact tcccttgact atcgcgcagt ggctcccggc tagttgcgac
     5341 gcctgagccg cgtccagagt tcggcctgtt tgatcgagcg gtcgagtcct tcgtagcgcg
     5401 aaaggagcgc gcggcggtcg aggacgtggc cttgcagcca gacatctgcg gcgagcgtcg
     5461 agccgacacg cagcgcaccg ccgcgcaggg agcgttgatt gatcaggact tcgagtttga
     5521 aatgttcgag gtcgagataa gcccagtaga gttcgccgcc ggagagcggg ttgcgcaggg
     5581 gcttgaagga gagcacgcgg ccacagaggc tccaatcgtt ttcgtgcgcg gcgcgcgccg
     5641 gggcatattc ttccagagct ttccagcgcg ggcgttcttc gcgggcgcac acgcgcgcac
     5701 ggtaggccag accgcacagg ccgacgtgca actcctgccc gttgaaaccc tcgaagccga
     5761 cttccgttaa attctgcaac tcgaagagca cttccgtctc cgtaccgtcg cagtagccgt
     5821 gcacgatggc ctcgccctct tcgtcgtatt cggtcaaagc ccaagggctg atgcgttgcg
     5881 tgtagcgcgc gcgaaaaccg gggcggcaat cggcgtaaaa aatatcgccc gtgcccgact
     5941 cgtagagcac cgtccaaact tccaatccct cgcccagttt ccagcagcga ccgtgcagca
     6001 ccgcgccgcg ccggagcaac tgcgacggct cgccctgatc gcccgcgcgc tctgcgagat
     6061 gattgaaatc acgctcgctc ggcacgtcta tcccgatagc ttcaaacgtc tcttccataa
     6121 cttaagtaca gcgacgagtg gcgagtgaca agtgacaagt taaagcagaa aacctgtgtt
     6181 gtgcttgctt gtcacttgtc actcgccact ggtcactacc tggtagctcg ctttcgtggc
     6241 tgaatgctct tcaacttctc ttctctttcg cccgcgcgcg gcgcgcgact tttgaagccg
     6301 cgaatgttgc gctcgggacc atggcttgga cgcgcgcccg tgcgcgtcat ctcggcgaag
     6361 agttccacat cgtagtcggc ctcacgcgac atctcttcgc ggatggcgtg caactcttcc
     6421 agaaatctcg gcttacgata catagctggt gaatagtaaa tcgtaaatcg tgaatagtaa
     6481 aaacaaaagc agttcttatt cacgatttac gattcaagat ttactaatcc ttcttttccg
     6541 tcaactcgga aaccaggaac tccggcgtga cgatcatcgg gacgaagaat ccggcggcgg
     6601 tgttgaagag acggatgcgt gcctgtctcc gcacgttcgc cagatgaccg aagttccatg
     6661 taactagata atctatttta tgcacggagg caaccgcgac gtgcaaggca tcgccgatat
     6721 acttgcgatg aaagatgccg cgcgagatgt agccttcggc gaggatggtg gcctcgtccg
     6781 tcaactcaag ttgtgcgagc ggcgcgatga gcttcagata agcggtgcga tgcggttcgg
     6841 gcacgcgttc gagttcgcgc atcacgagcg gcgagatcac ggcgcggtac tccgacatct
     6901 cgtgctccca ccagcgaatc gtcaggtcgc gccggaaagg ctcgccgttg tcgagatacg
     6961 caccgacgac ggacgtttcc agatagaggc tctgcttcac ggcgtcgccc ccgaagcggg
     7021 cgtgatgttg acgtcgcgcg tcggcggcga ggtgacaggc gcgggttgcg tggtgagcgg
     7081 atcgaggcgc acgaggccac gttgaaaatc gaacgtgacg cggaagctgc gcaggatgtt
     7141 gccgccgatg atgccggtct gttcgaagcc gcaagtctcg ttgatggccg acatgtcgag
     7201 cacggcggct gagaggttcg agtgcgtgta atcgccgagc gtgacgcgcg gcaaaagcag
     7261 cgtcatcaca tcttcggaga cgcccgccgc gccgaaaact ttgagccgcg tcttttgttc
     7321 gaagcgcgac atgtcttcgg tcttggcgag ttgtgtggag acgacagaga tgctcgcccc
     7381 tgtgtccacg atgaagttga gcggtttctc aactccgtcg agcgtaaccg cgctgctcca
     7441 aaatccgctc gacgtggcgc ggatgggaaa ctcataactg cggctcgtgt cgctgccggg
     7501 cgacgcgctc gacggtgcgg gcttcaaggt cgggcgtgac gtgggcgtgc cgagcagagt
     7561 cgagacggtg ggcggcggcg cggcgttgtc ggcaaaaacg ttgtcggcga gacggtcaat
     7621 agcgcgcggc gcagcggggc gcggggcgtc gtcgcggtct gcgttgcgca cgagcgtcat
     7681 ctcgcgcgtg ccgtagtcaa ccgaggtaac atatttggag aggacggaga ggccgatgta
     7741 accgtccact gcctcctgct ggttgtgaaa gttgcgtatg taaacgggca cgttctcgat
     7801 gcgcgccccg tctatttgca gcgacgagag gaagccgtag acgatggcga agcgtccgcc
     7861 gccgcccacg gcgcgcgcca tgccgccctc cgcgatgggc ttgatgccga gacgctctgc
     7921 cgtcgccgtc gagagcacgc acatgcctga acccgtatcc acgacgaaac gcagaggcgt
     7981 cttcgagccg ttgacgtgta cgctgacgat gggacggctg ttggagagtt cgaaagaaaa
     8041 ggtcgtgcgc ggcgcgccgg tcgtctggta gagattgcgc tgcgcgccga ggtagcgcag
     8101 gaaactgatc aggccgcgaa tacgtgcgcg ccggtcagcg tccgtgcgcg gcgcgatgtg
     8161 cagaaactct tcataagcgt cagccgcttc gcggtaacgc tcggagcgcg cggcggcctg
     8221 cgcgtaggag tagatgtaat caggctcttt cgggtcgaga tagatggcgc ggcgcaaacc
     8281 cgtgagcgcg accgaagcgc gattctcgta gaagttgatc atcgaaagcc ccgcaatcgc
     8341 caacgcctcg tcctccttga acacgagtgc ggtgcgaaac tcttcgatgg aattcttgaa
     8401 gtcgccggag gcgaggaggg ctgaaccgag cagggcgtgg gcgcgagccg aagtcggatc
     8461 gagggcgagc acgcgcgccg cctgttcgaa cgccccgcgc aggttgcgtt gcttcaacag
     8521 cgcgaaactc aaacccagac gcgcgggcac gtctcgcggg tcgcgctcca ccgccgcgcg
     8581 atagagcttc tcggcctctt caaactcgcc ttgccggatg gctcgcgcgg ccttctccag
     8641 attcttatcg cccgcccacg cgaacgcacg ggcatccgcc ccgcacatcg ccacacagat
     8701 caacgagaca gccaaactgt cccacaacac acggctcagt tttttcatct ttcggccacc
     8761 ccttcaggca agggatgagg gatgagggat gaaggatgaa taaaaccaaa gcccgtttag
     8821 cggcttgaat gtctcttatt catccctcat cccttatccg tcatccttat ttgagtattt
     8881 ctccggtctt cattgaccac agtaccagat cgagttcatc gaaatctatg gcgagacgcc
     8941 cggcaaaacg gcgtagtgat tcttcgatga cgaggtagcg tgcgcgggtt gtcggcggtg
     9001 ccggagattc gagcacgcca agttcggcga ggctacgcag gatgtgcttg tcgagaatgg
     9061 cgtagccgcg aaagcctatg ttgcgcaggt agtggctcgc ctctttgtat cccagacctt
     9121 tgatgcgccg ttcgcgtgcg agccagtcgc ggcgcgcgag gggatcgtcg aagctcagga
     9181 gtcgttcgcg caggcgcatg ccacagtctt cgcgcaggta gtcgcgcgtg acgacgatgt
     9241 aaccggagcg cgagttcggg taacgatgca cgccgccaag cgcgtgcgcc agttccggct
     9301 ccgcgccttc ggccagcagc gcgcgcaccg cttcgacgga acgcaaaccc atcctcgccg
     9361 acgctccggc ggtgaagatg caaaagacca actcttccca caggcgcgcg tccgagcctt
     9421 cgcgccacac gtcctgaaac tccgcgaggc ggcggcggat gtccgtcgcg cgcgcccggt
     9481 gtgtctcgcg cagggcttcg atggtgacgg gctgcttgtc tcggggcaca atgcttacgg
     9541 aagataaacc tcttgccgga ttgtcaattg cgaggcgcga tcaacatctc gaattttagg
     9601 agcgcgttcg cggccttgtc aacgagtttg caggttggag gtcgcgggtg tggaatttcg
     9661 cgggtcttaa tcttttatct caggacagca cacgtacatc ccccgataga cttgagagca
     9721 tcttaagtgt cgcgcttgcg cactccccgc cgagcattcg cgcgaacaca agatcactct
     9781 ctccgtgtta tgatgcaatt gcccccaaac aattgataga ggataaagat ggtcggatca
     9841 aaaatcaggc aattgttatt cgtgtcaacc ctgctgctct cgtgctacgc gagcgccgcc
     9901 gcacaggacg actccacgcc cgcgcctcag ccttctacgt cggcgacgcc ttacgtcgtc
     9961 tccacctggc cggagacgct gccggtgagg tcgtcgccgg acattcccga cctgctcgcg
    10021 gcgcagcccg cgccaacgcc cgtcaactac aatcagggag tgctcgtcga gacgattgac
    10081 gggcgtgtgg tgttggagca gggcgcgaac cagcaataca atccggcgtc ggtcgtcaaa
    10141 ctcgccaccg cgctcgatgc cctgcaaacc tttggaccca actatcgctt ttccaccgtc
    10201 gtctggacga acggaacttt cgatcaggcg acgggcacga tcacaggcga tctgatcatc
    10261 tccggtcgcg acccctcgct tcactacgag catgccgtcg ccatcgcacg cgaactgaac
    10321 cgcctcggca tacgcaccgt gacgggcgac ctgtacgtgc cgccgaagtt cacgatgaac
    10381 ttcaactggt cggcacagcg ttccggcgat cttttctatg acacgctcga ctcgacgcgc
    10441 cgccctgcgg cagcgacgcg cgcatggttc gacgagcgga tcgcgcacgg cgatcaggcc
    10501 gccacgcaga cgattccgag cgtcgccgtg atgggcgcgg tctatgtcgc ctccgtgccg
    10561 caaaacgcgc gcgtgctgat cacacatcgt tcaagcaagc tggttgacgt gctgaaagtg
    10621 ctgctctgct attcgaacaa cttcatggcc gaacgcctcg gcgacaatct cggcggcgcg
    10681 gctggcctgg agcattttct gatcagcaaa gtcgggcttg cgcccggcga tgtgcggctc
    10741 gcttcgacga gcgggttggg cgtcaatcgc ctctcgccgc gttcgatgat gaagatttat
    10801 cgcgcgctcc tgaaggaact cgacaaacat aatttgtcgc cctcggacat catgccggtg
    10861 gcgggcattg atccgggcac gttgcagaag cggtacacgt tcggctattc gcgcggcagc
    10921 gtgatcggaa agacgggcac gctcggacgc acggacggcg gcgcgagcgc gctggccggg
    10981 cagatgcgca cgaagaccgg cgagacgctg ctgttcgtca tcttcaaccg gggcggcagt
    11041 gtcgcgcgtt tccgcgaaac gcaagaccgt ctcgtctccg agttacaatt cacgcgcggc
    11101 ggccccgctc ccttcgccta ctcgccgcaa actctcgcca tgcgcctctc cgacaccgaa
    11161 ttcgaacagg ccgacgcgtc ggagtttgaa ccaaagacaa actgagggat gaaggatgag
    11221 ggatgaggga tga
//
LOCUS       DASWNS010000041         8619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_286956, whole genome shotgun sequence.
ACCESSION   DASWNS010000041 DASWNS010000000
VERSION     DASWNS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286956"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3131)
                     /locus_tag="VGB76_01430"
     CDS             complement(<1..3131)
                     /locus_tag="VGB76_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097588.1"
                     /translation="MPERIYNWRRYWYPRGSNVRFLDNGFLYSPGRFNQDVVPFEALD
                     DVPCLVLLGEPGMGKSRALEAERAAIEARVREGGGNAFWLDLRSYGSEDRLVRDLFRS
                     PDFLAWLEGNHLLHLFLDSTDECLIRIETLASLLIDEFNKYPVERLRLRIVCRPAEWP
                     ALLEEGLVRLWRRESVKIYKLAPLQREDALTAAAEEGLDAEAFLGEVDRMEVGPFAAK
                     PVTLKMLLRVYSRDQRLPQNQAQIYEEGCLTLCEELNPSYVASGRTGDLTATERMTVA
                     ARIAAVMTFARRSAIRTGVGAGEPLPEDITIDEIAGGNDESPDGFFDVPRAAVREALD
                     TGLFAARGPDRIGWAHQTYSEFLAAWYVSRRLSAAQVLSLIVHPDDAEGKLVPQLHEV
                     AAWMATNTREVFEAIMKADPEVLLRSDVTAYDDGLKETLVAELLKLYDEERGYVGWLS
                     NSRYDRLGHHRLADQLRPYIRDDAKNTMVRIVAIKIAESCQLRTLQADLADVALDASQ
                     PVDVRAYAARAVSQIGDEEGKLRLRPLAEAGIAEDRTGRLKGWGLRALWPGLITGQEL
                     FQLLTPMGVNDTGSYGHFVSGDLVQHLRPEDLPVALSWVEAQGDYRRLGFYFKRLADS
                     IMLSGWEHLDEPGVLEAFARAALLRLRYHNQIIGDHHERPDFESSVRAEDDKRRRLLE
                     ALLALISDTEQIRFLLIYTGSALVLSKDVPWMLERFETADSEEAREAWAQVIEAAFGR
                     GGGENYDLVLAAYRRDTLLSERLGWAFETVITDSQEARQMKEQYRMMHGLDVERDEDE
                     ESDQTAAGPSVEERIATFLDESEAGDADAWWRLNYVMTFGPEGNQGVGESESDLTATP
                     GWVASDDDTHVRIIEAAKRYVVEQGPQTEEWLEAGITYRPAYAGYRALRLLLDKDPEF
                     VAGLTPDVWGKWTPIILLYPTNDSSEEGLEPHLRLVELAYRSAPEEVIQILLGQIDRE
                     NSQGRYASIPDKVERCWDERLGGALLEKARAQEISAGSLGNILRALLKHGVEGAREFA
                     RSFITLPIPESGV"
     gene            complement(3199..3831)
                     /locus_tag="VGB76_01435"
     CDS             complement(3199..3831)
                     /locus_tag="VGB76_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097589.1"
                     /translation="MPDDGKIIELFASARRAEWAGDVEPARRLLQALKQAAVVRAREP
                     DEHPIAVILERAKLLDARLDRAPAEVPDRLVVQTANLRGVIWRTGERNFELRAYLLDP
                     SWEESLYRRAHAVAGRYRPLLTAVKGTDTEDVRDKKGQLRLYQDIERMKPDSGRGASW
                     AMDGDAFLREVTVGDFALVRIKMVWAQRELPENFLIIRKLLEEALRQLDE"
     gene            complement(3824..4591)
                     /locus_tag="VGB76_01440"
     CDS             complement(3824..4591)
                     /locus_tag="VGB76_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097590.1"
                     /translation="MEFLQEFYIGMTRYALGTGVSKHINPKIEISADQLETAADYAGS
                     CYILEMGQHYIEYLGYGSGFPLFPPITGYPMWAPHQLDTKSRASNVTGNIGETITGIV
                     VHRTLKFDSNRIAPLKAQPGVKTPDFLIARKPKFIQLLTDIESSLVSLRMPVWWPVES
                     KTRSDGFFTSDVKVGLRQLATYWYGIRRRYPAGVGYGVVIGADLKNPRRICIHVFVPK
                     DKPGLTTYLKSFSDNTKYSEDLDKQIGETGKYLMHYA"
     gene            4895..5044
                     /locus_tag="VGB76_01445"
     CDS             4895..5044
                     /locus_tag="VGB76_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097591.1"
                     /translation="MVKIDREKIVKAAVGSARLEGYKGPLKPLTEKGADVKRAEAKTE
                     PTVKR"
     gene            5053..5658
                     /locus_tag="VGB76_01450"
     CDS             5053..5658
                     /locus_tag="VGB76_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY0097592.1"
                     /translation="MADRYDTTGNSEDEYYPGTFVLINLEDIRDPEELLERETELQLA
                     AYEVMFGSFDETLTPALPFFYYLHHMMFASLYVWAGRSRTVGISKGGTPFCPPQNIEV
                     MMGALFSELGSEQWLAGLDNESFLRRAAYYVCEINAVHPFREGNGRAIRFYLDVLSAR
                     ARSDIFDWTLVGEDEYLQACIAGFAQNYSPMEAVLGRCAPR"
     gene            complement(5736..6620)
                     /locus_tag="VGB76_01455"
     CDS             complement(5736..6620)
                     /locus_tag="VGB76_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097593.1"
                     /translation="MKMRGTNLGGMRLGEDDLVAHLNDIIPGLTKRRVSSWRSKGLLP
                     AFDIQGKGAGRGRGRQISYWSQGEKIIEQAVAVHEVLSFYPSAEEAYLPLWLFGYEIH
                     VETARKKLLEEITAAREVLAEGEGSSDSLEDYFFDRAYDLFEALQVCDRESAKEFTFE
                     QVERFVQTLANPAYDPDDPPSIEWQFVREHFLLTVLYERVANLSLDELEQVRKDYRHV
                     IDAISLLASAFPSFPSEFPQKYLVGGNVGFFFVLFDLAFRRAGYGDFIDRHFPRLSTY
                     IQQGLEKREAAELLESRG"
     gene            complement(6961..8094)
                     /locus_tag="VGB76_01460"
     CDS             complement(6961..8094)
                     /locus_tag="VGB76_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097594.1"
                     /translation="MNVTTENLSVSKCIYCGREGRLSGEHYLPRCLGTFIGYELLNDR
                     VCKDCNNSFSPLDEQFCRSGPEAIIRDMLGIEGRKTHKRVSPFQRGSAGAERLKFTGK
                     SHSEDIEDGEFDLDVDRQTRAVRRMRQIIISAGERRVVLRVTDDIREPEQLIELMKKK
                     GVEMATPGDPDTEAVTAIFTGVAEDEVEWMQHLLSGLKPNISEGPEIKKGEPEQETRA
                     TMGATPTEKYFRGLAKIGFHYFLKYMRGFHGSEATFTGIRDFITDGKAEDVFRFISGW
                     TNHRVVDITPGESRSGYRHLLLAYSNYERLLCKMQFFVHPKHSSPIYRFDRLTPPIYT
                     INLGRNPSRVDYPQAKRHSFTYFNTPGENGYDGEMKEEFTRAR"
     gene            complement(8224..>8619)
                     /locus_tag="VGB76_01465"
     CDS             complement(8224..>8619)
                     /locus_tag="VGB76_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097595.1"
                     /translation="CAKDTSEEVMETLGHSLPGFETSEITRRQQIFRPVAQRLQADDR
                     LMGLMITKMGQNPSPTEKATLSRLIAQARGAEAIRTWCVNELTKQTLPTTASEIGVDI
                     SIGDFVPVTHVLLDILNDQYEPHQNQFLD"
BASE COUNT         1844 a         2542 c         2197 g         2036 t
ORIGIN      
        1 aacactccgc tttcgggtat gggcaaagtg ataaaagacc gtgcgaattc tctggcaccc
       61 tcgaccccat gctttagaag cgccctgagt atgttgccga gactcccggc gctgatctct
      121 tgagccctgg ccttctccag cagggcaccc ccgaggcgct catcccagca acgctcgacc
      181 ttatcaggga tggaggcata cctgccttgg ctattctccc tgtctatctg cccaagcagt
      241 atctgtatta cctcttctgg ggcacttctg taggccagtt caaccagcct caagtgtggc
      301 tccagaccct cctcgcttga gtcgttcgtc ggatagagaa ggatgattgg cgtccacttc
      361 ccccacacgt caggcgtcag tccggcgacg aactccgggt ccttgtcgag tagaagtcgc
      421 aacgctctgt atccggcgta agccggccta tacgtgatcc cagcctccag ccactcttcc
      481 gtttgcggcc cctgctcgac gacatatcgc ttagccgctt cgattatcct gacgtgggta
      541 tcatcatccg acgccaccca cccaggcgtg gcagtaaggt ctgactcgct ctcaccaacc
      601 ccctgatttc cctccggacc aaacgtcata acgtaattga gccgccacca tgcgtctgcg
      661 tcgccagcct cgctctcgtc taaaaacgtg gcgatccgct cttcgacgga cgggcccgct
      721 gccgtttggt ctgactcttc gtcctcgtcc ctctccacat cgagcccgtg catcatcctg
      781 tattgctctt tcatctgccg ggcctcttgc gagtcggtta tgaccgtctc gaaagcccac
      841 ccaagcctct cggacaagag agtgtcgcgc cggtaggctg ccaagaccag atcatagttt
      901 tccccgcctc ccctgccgaa ggccgcttcg ataacctgag cccacgcctc gcgcgcctcc
      961 tccgaatctg ccgtctcgaa ccgctcgagc atccaaggca cgtctttgct cagcaccagt
     1021 gcagagccgg tataaatgag taagaaccgt atttgctcag tgtcggagat gagcgctagg
     1081 agcgcttcaa gaagcctgcg acgcttatcg tcctcggctc gaacggacga ttcgaagtcg
     1141 ggccgttcat ggtgatcacc aattatttgg ttgtgatacc tgagtcgtaa taaggccgcg
     1201 cgggcgaagg cttcgagcac gcccggctca tccaagtgct cccagccgga tagcatgatg
     1261 gagtccgcca gccgcttgaa gtagaacccg agcctgcggt agtccccttg tgcttcgacc
     1321 cagctgagcg cgaccggcag gtcttcaggg cgaaggtgct gaacaaggtc tcccgacaca
     1381 aagtgcccgt aggaccccgt gtcgttcacg cccatagggg ttagcagttg gaagagttct
     1441 tgaccagtaa taaggccggg ccacagcgcg cgtagccccc aacctttcaa acggccagtc
     1501 cggtcttcgg ctatccccgc ctccgccagc ggcctcagcc tcagcttccc ttcttcgtca
     1561 ccaatctgtg acaccgcgcg cgccgcgtac gcacgaacgt caaccggctg agaggcatcc
     1621 agtgcgacat cggcaagatc agcctgtaac gtccgcagct gacagctttc tgcgatcttg
     1681 atagcgacga tgcgcaccat ggtgtttttt gcgtcgtcgc ggatgtaagg tcgtagctgg
     1741 tcggctagac ggtgatgtcc taaacggtcg taccggctgt tgctcagcca gccgacgtag
     1801 ccccgttctt cgtcgtacag tttgaggagt tcggcgacca gcgtctcctt aagcccgtcg
     1861 tcataggccg tgacgtcgct tctgaggagc acctccggat cggccttcat gatggcctcg
     1921 aacacttcac gagtgttcgt cgccatccag gccgccacct cgtgcaattg cggcacgagt
     1981 ttgccttccg catcatcggg gtggactatg agactcaata cctgcgccgc gctcagccgc
     2041 cggctgacat accaggcggc gagaaactcg ctgtaggtct gatgcgccca gccgatgcga
     2101 tccggccccc gcgccgcgaa cagtcccgtg tcgagtgcct cccttacagc cgcgcgtgga
     2161 acgtcgaaaa aaccgtccgg cgactcgtca ttaccacctg ctatctcgtc gattgttatg
     2221 tcttcgggaa gtggctctcc cgcgccgacg cccgttcgaa tcgccgaccg tctcgcaaag
     2281 gtcatgacgg ccgcgatgcg tgctgcgaca gtcatgcgct ccgtggcggt gaggtctccg
     2341 gtgcggccag aagcgacata gctcgggttt aactcttcgc agagtgtaag acagccttcc
     2401 tcatagattt gggcttggtt ctgcggtaag cgctggtcgc gactgtaaac ccgtaacagc
     2461 atcttcagtg ttacaggctt agcggcgaat ggaccgacct ccattctgtc tacttccccg
     2521 aggaaagctt ccgcgtcgag cccttcttcg gcggccgcag tcagagcatc ctccctctgt
     2581 agtggggcca gcttataaat ctttactgac tcacgtctcc acaatctgac caatccttct
     2641 tcaagaaggg cgggccactc ggcagggcga cagactatgc gcaggcgaag tcgctcaacc
     2701 ggatacttat taaattcgtc tataagcaga gacgcgagtg tctctattct gatgagacat
     2761 tcgtcggtac tatcaaggaa gagatgtagg aggtgattcc cctccaacca ggcaagaaag
     2821 tcgggcgacc ggaaaaggtc acggacgagc ctgtcctcgc ttccgtatga gcgcaggtct
     2881 agccagaaag cattcccgcc gccctcacgc actctagctt caatcgcagc ccgttccgcc
     2941 tctaaggcgc gactcttccc catacccggc tctcccaaga gcaccagaca cggaacatcg
     3001 tcgagcgcct cgaatggtac gacgtcctgg ttgaaacggc cgggactata aaggaagccg
     3061 ttgtccaaga atctgacgtt gcttccacgg gggtaccagt atcgtcgcca attatagatt
     3121 ctctcgggca tcttctactt taaccaagcc agatcgatta gttcccttgt gagtaaccca
     3181 ttcgatacgt cagtacgtct attcatctaa ctgtcgcaac gcctcctcaa gaagcttccg
     3241 tataattaaa aagttttcag gcaactccct ctgcgcccat accattttga ttcttacgag
     3301 cgcaaaatcg cctaccgtta cttcccttag aaacgcatct ccgtccattg cccaagaagc
     3361 tccccggccc gaatccggtt tcatccgctc tatatcctgg tacagacgca attgtccttt
     3421 tttatcccta acatcttccg tgtctgtacc cttcacggcg gtaagcagtg gtctgtagcg
     3481 accggcaaca gcgtgggcgc ggcgatagag tgattcttcc caagatggat ccagcaagta
     3541 agctctcagc tcaaagtttc tctccccggt acgccagatg acgccgcgca aatttgccgt
     3601 ctgtaccacc agtcggtcgg gaacttctgc gggtgcccgg tctagacgag catcgagtaa
     3661 cttggcccgc tctaaaataa cagcgattgg atgctcgtct ggttctctag cccttacgac
     3721 cgccgcttgt ttaagtgcct ggagtagacg gcgggcgggc tctacgtcgc cggcccattc
     3781 ggcccgacgg gcactcgcga atagctctat tattttgccg tcatcaggca tagtgcatta
     3841 gatacttccc ggtttcgccg atctgcttat cgagatcctc gctgtacttc gtgttatcgc
     3901 taaacgactt caggtaggta gtaagacctg gtttatcttt gggcacaaag acatgaatgc
     3961 atatccgtcg cgggtttttc aggtcggccc ctatgacgac gccataccct acgccggccg
     4021 ggtaacgtct tctgatcccg taccaataag tggcgagctg tctcaaccct acctttacat
     4081 ctgatgtgaa gaagccgtcg ctgcgcgtct tagattctac cggccaccag acgggcattc
     4141 tcaggctaac tagggatgat tctatgtcag tcaggagctg aatgaattta ggctttctgg
     4201 caatgaggaa gtcaggggtt ttaacgccag gctgtgcttt aagcggcgct atcctgttcg
     4261 agtcgaattt taaagttcta tggacgacaa tccctgtaat agtttccccg atattccccg
     4321 tgacgttcga cgcgcgcgac ttggtgtcca gttggtgcgg cgcccacatc gggtagccgg
     4381 ttattggcgg aaacagcggg aaccctgaac catatcccag atattctatg taatgttgcc
     4441 ccatttccag tatgtaacat gagcccgcat agtcggcagc agtttcaagc tggtcagcgc
     4501 ttatttcgat cttagggttt atgtgtttag acacccctgt gcccaaggcg tagcgcgtca
     4561 ttcctatata gaattcttgt agaaattcca ttccgtgtcg ctcgtcacca taaaaattta
     4621 gggcgaatta tagtcgagaa accgttcttg caatacaaac taccggttgc cggtgtaaag
     4681 ccggcgacct attcaagacg gggatactat tcttgagggc ggtatcccat gtcttcaact
     4741 acgaccgggc agattgggga catgctctat acccccggca ctctttacag tattcacctt
     4801 gtttttgacc attgaaatcg ttcataaatg ccagcaaaaa tttcggcggc ggtgtaaaat
     4861 atgaggtgct ggattgcgac ggagggcgag gcacatggtt aagatagacc gcgaaaagat
     4921 agtgaaggcc gccgtaggct cggcccgcct tgaaggctat aaggggccgc tcaaaccgct
     4981 gacagagaag ggcgcagacg tgaagcgggc ggaggcgaag accgaaccca cggttaagcg
     5041 ttagccacgc ccatggccga ccgctacgac accacgggaa actcggagga cgagtactac
     5101 cccggcacct tcgtcctcat taaccttgaa gacatccgcg acccggaaga gctgctcgag
     5161 cgcgagacag agctgcaact agccgcctac gaggtgatgt tcggctcgtt cgatgagact
     5221 ctgaccccgg cccttccctt cttctattac ctgcaccaca tgatgttcgc ctccctatac
     5281 gtgtgggcgg ggcggtcccg cacggtagga atctcgaaag gtggcacccc gttctgcccg
     5341 ccccagaaca tagaagtgat gatgggcgct ctcttctctg aattggggag tgagcaatgg
     5401 ctcgccggcc tcgacaatga atcattccta aggcgcgcgg cctactacgt ctgtgagatc
     5461 aacgccgtcc accccttccg cgaggggaac ggacgcgcca tcaggttcta tctagacgtc
     5521 ctgtcagccc gcgcccgcag cgacatattc gactggacgc tggtcggcga agatgagtac
     5581 ctacaggctt gcatcgccgg gttcgctcaa aactacagcc cgatggaggc tgtgctcggc
     5641 cgctgcgctc cccgctaaca acccgcacca tttaacttgc ttacagcgca ccggattact
     5701 gttctagtcg agcgcacgcc aaactcaatc aacgctcagc cccggctctc taacagttca
     5761 gcggcttcgc gtttctccaa accctgttgg atatacgtcg acaggcgcgg gaaatgtcga
     5821 tcaatgaagt cgccataacc agcccggcgg aaggccaaat cgaagagcac gaagaaaaat
     5881 ccaacgttgc cgccgactaa atacttctgg ggaaattctg aagggaatga ggggaaggca
     5941 ctcgccagca aacttatcgc gtctatcacg tgacggtaat ccttccgcac ctgctccaac
     6001 tcgtccaaag ataaattagc caccctctcg tataagaccg tcagcagaaa gtgctcgcgc
     6061 acgaactgcc attcgatgga tggcgggtca tcagggtcat atgcagggtt ggcaagtgtc
     6121 tggacgaacc gttcgacctg ttcgaacgta aactctttgg cggactcccg atcacatact
     6181 tgtaatgcct cgaacaggtc gtaagctctg tcgaaaaaat aatcctcaag gctgtcgctg
     6241 ctgccttcgc cctcggccag tacttcgcgc gccgcggtga tctcttcaag cagttttttt
     6301 cgcgccgttt ctacatgaat ctcatatccg aaaagccata agggcaaata tgcctcttcg
     6361 gcgcttgggt aaaaacttag gacctcgtgt acggcgacgg cctgttcgat aatcttctcc
     6421 ccctgactcc agtagcttat ctgccgcccg cggccgcgcc ctgctccctt gccctgtatg
     6481 tcaaaagcag gcagtagccc ttttgagcgc caactgctga cccgcctctt cgtcaatccg
     6541 ggaataatgt cgttgagatg ggcaaccaga tcatcctcgc ccagcctcat gccacctaag
     6601 tttgttcctc tcattttcac tgccacgctc cttacctact agaattagga cgccacttgg
     6661 cgcgtctagg aacggagcat agcaaacagg catcgatgaa aattgcacct gcggtgtagg
     6721 ttgtaaataa tctttaggtc ggggctgatg gtatgacagc atgaacactt ctaaaccacc
     6781 ttgacgcggc aaagtcactg ttaggcgacg acttaatgtg cgtctgagtc ccgagcgttt
     6841 gatggatcga caaccggaag agttttctta ctacacctag agaagtgtag tgaaattcgc
     6901 ctacagcatc atatccgttg actctgcgtt ggataatttg cgctgccaat gcgctgatag
     6961 ttagcgcgca cgtgtaaact cttccttcat ttcaccatcg tacccattct caccaggtgt
     7021 attgaagtaa gtgaatgaat gtctttttgc ctgcgggtaa tcgacgcggg acggatttct
     7081 tcctaagttt atggtgtaga taggaggcgt gaggcgatcg aatcggtaga tgggggaaga
     7141 gtgcttaggg tggacaaaga actgcatttt gcacaggagt cgttcgtagt tggaataggc
     7201 aaggagcaga tggcgatacc cggagcggga ttcgcccggc gtgatgtcga ccacacgatg
     7261 atttgtccaa ccagagatga atcgaaaaac gtcctccgcc ttaccgtccg tgatgaaatc
     7321 acgtatacct gtgaaagtgg cttcggatcc gtggaatccg cgcatgtatt tcaggaagta
     7381 gtgaaatcct atcttggcaa gccccctaaa atacttctcg gtcggggtgg cacccattgt
     7441 ggctctagtc tcctgctcag gctctccctt ttttatttct ggcccttctg aaatattggg
     7501 tttgagtcct gagagtaggt gctgcatcca ctccacctcg tcctccgcga cgcctgtgaa
     7561 tatcgccgtt acggcctcag tgtcgggatc gcccggcgtg gccatctcca cacccttttt
     7621 cttcattaac tctatgagtt gctccggctc ccgtatgtcg tcagtaacgc gtaacacaac
     7681 gcggcgctct cccgccgaaa ttataatttg cctcatccgc cggacagccc tcgtctgcct
     7741 gtcaacgtcc aagtcaaatt ctccgtcctc gatatcctca ctgtggcttt tgcctgtgaa
     7801 cttgaggcgc tcagcgcccg cactaccgcg ctgaaacgga gagacccgtt tatgggtctt
     7861 ccgcccttca atccccagca tatctctgat aatggcttca ggaccgctgc ggcagaactg
     7921 ctcgtcgagg ggtgagaaac tattgttaca atccttgcaa acccggtcgt tcagtaattc
     7981 ataccctatg aatgttccca gacatctggg tagataatgc tcgccggaaa gcctgccctc
     8041 acgaccacag tatatgcact tggatactga aaggttttcg gtcgtgacat tcatttggtg
     8101 gacgtatacc aacgcgctat gcgaacactc actgcggcag aacagataac ttgcgatgct
     8161 tacagctggg cggaatcact tacggggcgc gttcacttgg ctgctctgtt cgacagcaca
     8221 tcgctaatcc aagaattggt tctgatgtgg ctcatactga tcgtttaaga tatctaacag
     8281 aacatgtgtt acaggtacga agtccccaat tgaaatgtcc acaccgattt ccgaggcggt
     8341 cgtcggaagc gtctgcttgg tcaattcgtt aacgcaccac gttctgatgg cttctgcccc
     8401 cctcgcttgg gcgatcaatc ttgatagtgt cgccttttct gtcggggaag gattttgccc
     8461 cattttcgta atcattaaac ccatcaaacg atcatccgct tggaggcgct gggccacagg
     8521 acgaaatatt tgctggcgtc gtgttatctc acttgtttca aagccgggca ggctgtgccc
     8581 cagcgtctcc ataacctctt cgctcgtatc tttggcaca
//
LOCUS       DASWNS010000042        19198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_1624, whole genome shotgun sequence.
ACCESSION   DASWNS010000042 DASWNS010000000
VERSION     DASWNS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19198
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VGB76_01470"
     CDS             <1..756
                     /locus_tag="VGB76_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097596.1"
                     /translation="INIHPFKRDLLDAWWTGFYGIKSNHAQWWERSSLRLWLRFFELI
                     RKYKIPIPRNMVRMIRATLLYDTVAARLYSRINVFKEFEKYTQDVARRAKRQIVESTA
                     RQLMLGPDDANFLRFRQVLDIGDDILVRVRRYLDNQDFTFQQLAGKVYTAIRAIFRMF
                     MLSAGAGLIAAVVAATLYVLNVLQPSYEILQARTWNPFGEQFILTKDDWEFTLPFSTG
                     ALQVIILIWFIVTAALLLMYGRRIYLRFGDVDD"
     gene            880..1407
                     /locus_tag="VGB76_01475"
     CDS             880..1407
                     /locus_tag="VGB76_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097597.1"
                     /translation="MANFKFQNIARETILFETNTEGQKSWQEQLKDILVLTREAYSLN
                     LAPVGLGGKVAILMTYEEPTLCTVRLCFRVIDQRGEPVSCGFQTMILLHKDTHEMVPA
                     PPLLTHFLTTESKVSLLEELKNPSFAERVAGGSRELKNVFPESIRRLGASVANSPREK
                     AYPKILDESQNEYSL"
     gene            1450..7854
                     /locus_tag="VGB76_01480"
     CDS             1450..7854
                     /locus_tag="VGB76_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0097598.1"
                     /translation="MRSISSSTSKTDPKVTFTFPGQGSYSHTLLRELYTAYPQTTPYF
                     RQADAITRELLKADFLPLVTAASAAEHDERLEGCPDLDQVGIYLAEVLIAGILIESGV
                     KPSLLVGHSFGELAALATAGVYSIETGLRIVCRRVLALQSAGASGGMAALSCDLERAT
                     QFLAESGSNSIEISVINHPRQTVLSGKLSELESLRPVVNRQGVSLTILKSRYPYHSSL
                     LAGAVEPFRAALDEFDYSPAEIPVYLGLEGMLYSPGFDLPQILSSQFIRKLHFSNVVK
                     VLYESGYRTFIECGAGEIVTKLIGQNLAQVASGIVAVPSAPQEGSLDEELRKLFARGF
                     GKEAATLAASETHDATRSSQPPAQLLESMSLVVQSMSQLVEHTSRLIEQVSTSLVTAN
                     LPVAAREENISPASRESVAQPDPVQPETLPADAAFVPVPPPRGNGSSPESSHLVTHAS
                     GSNGNNGSGAQPERKRELEAAFAREECAATPIAVVSLGCVLPGANDPEQYWSNILNGV
                     SGITNLADIDPTAAEDFLLTNEGDGIKVVPDKTYTLLHGSIVDIPYDAALLSKAYSRE
                     QFDVLTKGQKILALAVAQSLARLKVKIDPSASVKMQCILGATADGSKEFDEALFVESL
                     SMHLGALDEPESLRRAFADTLEEITGYRNGDTKKLTQHKIYASVIERLMGSGLKTYVV
                     DTACSSSLYSTYLGMRALQNRECDMVLAGGVFAPGPANNTLFAQFKGLTHRESRPFDA
                     DADGVVFSDGAGIVILKRLPDALADGDRFLAVIRAIGLSSDGKSPSINVPQVKGQGLA
                     IRHAYKDSAIDVDTIQYVEAHATATLVGDAVEFNALKDTMQRSPASPPVELGSVKALV
                     GHTGWAAGMASLIKICKAFEARVIPKQYNYVKPSPEIDLAHSQFTITETSHPWTENVA
                     PYPRRAAINGFGFGGTNAHLILEDFDEAYHRNLCANLDVKEKVPVELAVISIGSLFPG
                     TDGALAAEDPSTESRFRRDALRLPAKKMLLPDVTEHMDASQYLAALAAEKAFATMPEG
                     WTRFKNAMGVVLGLTSKTERGMRANERVFVDRLRRQVTQRGGSGNLSNADLTRILEKL
                     VESIRARNVPSGPYTLPGLMPNVTAGRIANMFDLNGPNIVVDMGDDSLLQALFVARQL
                     LAHDACTIVLAGGVNANNAADASEAEAVFLLALTTPEIARREELSILSVISFSGSEEG
                     THAGERPVSVSHPALNYKGAQGAPEMLKAIRQAREKNSLCEFRQPGEGALAGRRLIFA
                     SPASHAPAKTAQPTETQRDASRAYAFVQGTPIRYYTPQLAPAKATGDAKSLQERKILF
                     LVDQPEQWLTLENSGALAALDYHVVCPSGVRVAKSLPVDLTSEESIESSLSGLAGLEF
                     DTLIAVKTLAGQTKETLLLNNSEGELRWLDLLFAVCRQSYERIQRECIPVVAVCQGAY
                     QNGELDPFTGLAAGFMKSLARELGENVCRAINTDEVNFYKTLRQVEIELGQAGEDVEV
                     CYREGNRSVFTLAPVEKLAKDEQPQLDADSVVIATGGARGVTAVLAEELLEKFGCRVI
                     ALGRSDPSSMPEEVRTMDEQAFKEYEPQFYKDELARNKGKKMGELKREYLAYRAANEV
                     CQVTRRLQAISGKYEYRSVDITSEKAIEEVVAETYRKYGRVDLVLHGAGVQVSKALTR
                     KSLTDFRKIVATKLRGLSNLYKACRKYGEGRRIHFHILTSVFSYMGNDGQPDYGAANE
                     AMSRMAACMNSPENGNHWSAMAWLGWAGIGMTRDSEFAALAASRRLRGVTKEEGQQIF
                     SELMRGTPATPINILLADGEIEYYKVAIQTSSPEASPRASQASAQAAPPAVSQAVKAK
                     PDFHVMEREISVESSPYLLNHLIDGVPTLPGALVISLFAEAAQQLRPELKINSFEKAF
                     FHRFIKVYQNRKTRIRVEARIVSEDERQTVVQVRILSDFVHSSGVVLQKEILQHEILI
                     RMSSAALTSPARVHPNGLEGRRLLDPYVMDGSPVCLSGPFNAMENIIVGGAQRKAEFK
                     LADFNGASPAYKSEWSKIILMDSLWRFGVIHLAPDDSSPVFVPEECRVMKVYFDFSDF
                     DASTLAGKLTFSGVNPRLDGDRLTIGPVTAADGNGNPLIIVEDGICRRYGKVSNGNGT
                     SH"
     gene            7832..8692
                     /locus_tag="VGB76_01485"
     CDS             7832..8692
                     /locus_tag="VGB76_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY0097599.1"
                     /translation="MATAPHTKTEATAARFDDCFVGWLDVCELQDREYTSHLSVDERA
                     KYLSLRGERRKSEWLAGRLAAKHLFLNWLEPAREIRSGQWNPGLSRLSGEALAAYSPW
                     MYRKVEVLSDGEKPGLVWCGRKRKESLSISHAGGISCASIALEKPVAIDVETAVDRHG
                     AFYRNTFTEVEKNWVARVGGDDGLSSANWCFTLLWSLKESVLKLGWLDGASIWNLSRI
                     EIDGLPGLNQPGFNRREPFWRSRNLSDAFVVFTARVKEPRRAMRVQVAVTGTRNFVLT
                     VMNPLIGAVN"
     gene            8689..10197
                     /locus_tag="VGB76_01490"
     CDS             8689..10197
                     /locus_tag="VGB76_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0097600.1"
                     /translation="MSTAYTLPVANDLNSAVREIFAQVTRYPLEVLDPASGLEEDLGI
                     DSVKLGEVFAVLREKYGLPEKLDFPREKLKTIGGIAEALQVYLRDASPPAHAGGNGGS
                     NGSAGLAQPHSGNGNGNGNGNSPVDAATLQAGVLEIFAQVTRYPLEVLDPAAGLEEDL
                     GIDSVKLGEVFAVLREKYGLPEKLDIPREKLKTIGGIAESLHEYVSHSVAPPQVVKPP
                     SPPPSPAVISGGNGAHLSSTIAESFASLMPERKPFAGKVALLTGSGRGLGKDVACYLA
                     ELGASVIVNSFHSRQEGERTVEEIKAAGGDAIHAWGSVANPEHVKTIFDKIEETHGVL
                     DFFISNASNGMLARLKEITTEHWEKAFRTNVVGLHQTSLKAVELMKRRGGGKIITLST
                     PASHGYVDYFGCMGAVKAAVESLTKSMAIEFAADNIQVNCVSPGPVYGDLLNKWPESE
                     RLIRQWENNTAYSRLCEARDVSHFIAYLLSEPVKLFTGSVLIMDGGISSQGW"
     gene            10198..11265
                     /locus_tag="VGB76_01495"
     CDS             10198..11265
                     /locus_tag="VGB76_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1702 family protein"
                     /protein_id="HEY0097601.1"
                     /translation="MVGREFMSLAIHTNGNLSRFNRLKGRLLGLSPKEASFSRRAFPG
                     CGSGSREHLESVIRTFIEGYNLAVRESDPVRLAQRLDSTFAPAFVGFAYEGVGLYFGL
                     VDLLLPRSGSRLDAFTHSAGERHDFIISVGAGFAVARVPLGLRRLESYQKSLDPMTAW
                     CLADGYGFHEGFFHWKRFVDGRQPAPASLSLQNRRLFDAGVGRAMWWVYGADAASIAA
                     AISRFDAERRHEMWAGIGTALAYAGGVTPEAASLLHDLAGSYQSDLLSGIPFAAHMRD
                     KGRNPAAWTDEVCMGLLRMSVGETSEIIVTELAAYLDNWQGSEPDKWSGCYVALRERV
                     RQRLLKRRATPSANFALTNGD"
     gene            11276..13024
                     /locus_tag="VGB76_01500"
     CDS             11276..13024
                     /locus_tag="VGB76_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY0097602.1"
                     /translation="MTTNTDYDVIILGTGIGGSMLGAILAKHNLNVLMLDAETHPRFA
                     IGEATTPDTNFRLKLLSHKYDLPEIAHLSAFHPTRDFISPACGVKRAFSFLYQREGKD
                     QLPMETHQYPTLAPPMGPDCHFFRQDTDAYMMAVAVNYGAKVRQQTRIKDFDIQEDHV
                     SLTSEKGETFTAKYLVDGTGMKSVLAHKFELRKPAETFRTNTRAIFTHMVGVKLYDQV
                     GSSQQTHGHKYPLSQSTLHHVFEGGWCWIIPFNNHQDSTNPLCSVGLVLNREIHPETG
                     MNPEEEFWSFVKKFPDMVRQFEGAQAVRGWVGTGRLQYGATSITGHRYSLLSHAGFFI
                     DPLYSTGLALTTAMVDLLGGQLLRAFKNNDFSVESFEHLNNFFDSNIGYADEMVSSSF
                     VSFRDFELWDAWFRVWVVGLFIGTCLNASLYLKYIESKDKRVLDQSGKEPYSVLLGGK
                     FPEFQKLYREALAEMDRVKAGQVDPKEAAQKIRGLFKGLDYVPTYWRWHEAVVRTTPA
                     FTIWGMTKMYFWFLFYAPKPIREALFGWSSFTAYKYILGSILDNNGASKRRKRSYIRD
                     VFKSWNLDWLPAKSHE"
     gene            13248..13670
                     /locus_tag="VGB76_01505"
     CDS             13248..13670
                     /locus_tag="VGB76_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097603.1"
                     /translation="MFNIPLLIAAGVALVAISLHIYTFEVWIWPKLKDDCFPSFPFGG
                     PDVVKGFYRTVWHFFTVNLLMTIIVNFMVGIGTLIPYGLLLIYYLIVFWILIFIEIWL
                     IGALSLRPGQSYLKTMLKAFQWVFVLLMIIFMYWGTRK"
     gene            13721..15973
                     /locus_tag="VGB76_01510"
     CDS             13721..15973
                     /locus_tag="VGB76_01510"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HEY0097604.1"
                     /translation="MKRLFAWIDSVVNKLATNFTLWIVGGIVALIAVAYTAYATVFVR
                     LPEPPVYAKYVELQPEEEEAGRKHGWNADRRQRYYQTSQGSLVMPYAWFRAVEWRTST
                     EMFASPQVQTRYGLLPDNDPVYNRDQMPVGLVKHIVPDQYVDNLGEGEKEWASISCAA
                     CHTGQILYKGTAMRIDGGQSFWNFDKWSGDMVFSLMLTSASPQRFERFCARVYGRRPG
                     EKCASNEVTTLRAQMKRYFNSDLIKSAIEALINHTYPTTEGFTRTSALGRGVNGEFGS
                     LDPCEGPLDRSCNRNVNRNTGPVSFPPLWYTHEYDWVQSTTAISQPLGRNVTEAWGVS
                     MRVELKDPSTRFASTANVEDMFWMETLISILTAPKWPEDILGKIDWERVKRGKYLYHD
                     AVWNKALPAAQAELQPDPASLIGAPNPNRPTTGLCARCHAPAFEAQPALTAALAQAPG
                     TNPNQYEKRFLQLPLYRQEVMGTDDDDAVQFNERRVHPGVLKELFKQEQFDDEGRIGI
                     GDMLTVTINGILDKWFKEHNIGEPCKTILQGYRPNQFRAPLGYPARPLDGYWTTGPFL
                     HNGSIRTMYELLSPAAERAEKFWIGSREFDPVYLGYKDEPVEGAFSFDISHQGNSNAG
                     HEFRDAPPNTVGVIGPALTHEQRLDLIEYLKVISTVPELLDNDPATRERLAERNGMLD
                     ALTPFYEKNAGWVTYGKNKPESEGGYSRMDFCKNIEAAAMRQSYAAPSASPSPAPSAS
                     PAATPYRKKR"
     gene            15980..17032
                     /locus_tag="VGB76_01515"
     CDS             15980..17032
                     /locus_tag="VGB76_01515"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0097605.1"
                     /translation="MSRLNDMRISAIGAPPQGELHETRLDIPFTIINITTTIAACVLP
                     FYFYSRSSIAAFLIFYTITGMGLTLGYHRLFAHRAFKVPRWLERLIALCGYLAIQRGP
                     IFWVAMHRLHHRYSDIPGRDPHTPREGWLHVHFGWHQRRRRDVWESEIYRRYTPDLVN
                     DRFYLFLDNERRDYFTYFGILFGSYLVGGLVGAFGTTGFQFDHYNAVCFLVWVGLLNR
                     VAVLHAFGFINSVCHELGTRPFKTRGTDTSVNNVIVSLLIFGEGWHNNHHAHPKSARQ
                     GLRWYQWDPAWYGIWVLKTLGLAEKVHVPSSEVIIRSSRSYVVPAGEGLARAEAVGGS
                     YERKTARGKVEPLEDA"
     gene            17034..17648
                     /locus_tag="VGB76_01520"
     CDS             17034..17648
                     /locus_tag="VGB76_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097606.1"
                     /translation="MKIYFGKVYGAHMDKLKTIWLPRGKRVYVWQGLISTDQRAYDQL
                     TAIAPNNAGGRPARSGRQNALLFKELEENNRWARLGANIYFGWFTLLLIINVIAAIWL
                     FTYRGVRPPYARLVFLLIIGMNLTGTVVTFFIRKHMLDCDRRIKGVLEELRQEPAAIG
                     AYDEPQSPVPRQVVNVMFAFTGVIFFMLFLFWMILEIWPHVFLT"
     gene            17749..18792
                     /locus_tag="VGB76_01525"
     CDS             17749..18792
                     /locus_tag="VGB76_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family protein"
                     /protein_id="HEY0097607.1"
                     /translation="MKRDAQLELNKEYPAPDEREITEKLIVLLKGIIEQRYLTGVTFR
                     DVHVKGHAGVRAEFIVEPNLPEEFRVGVFREARTFPAWIRFSNSSQDPAVDIKDDIRG
                     FALKLMGVEGEKLIESQKDATTQDFIFLSTNLFLTKDAKDFYELVECGALNKKKSLSD
                     YLGILGYFARHPDVGVKLLAALRKFPHLLEMEWFSATPYLFGERAVKYALRPWLKTKT
                     ALPRKPGNNFLRERLTEHLAREGTGFDFMVQFQLDPYREPIENSLVPWKEENTPFHKV
                     ASINIPRQKIDSPEQMSFAENLSFNPWHSLPEHRPLGSANRVRKEVYIAISEYRRSRN
                     GVPNTEPTPDESF"
     gene            18940..>19198
                     /locus_tag="VGB76_01530"
     CDS             18940..>19198
                     /locus_tag="VGB76_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097608.1"
                     /translation="MLEKIVNFLKSLKLWKKIALGVLLLLVVGVPLAYYLYNRDRAQP
                     LKPSAEVLYLNNEVWTEAERQRYYHLSQGAQIMPYDWLIALE"
BASE COUNT         4025 a         5595 c         5748 g         3830 t
ORIGIN      
        1 atcaacattc acccgttcaa gcgcgacctg ttagacgcgt ggtggacagg cttctacggc
       61 atcaagagca atcacgcgca atggtgggaa cgcagttcgc tgaggctctg gctgcggttc
      121 tttgaattga tcaggaaata caagattccc attccgcgca acatggttcg catgatccgc
      181 gccacgctgc tctacgacac ggtggccgcg cggctctata gcaggatcaa tgtcttcaag
      241 gaatttgaaa aatacacgca ggatgtcgcg cggcgtgcca aacgccagat cgtagagtct
      301 accgcgcggc aactcatgct cggtccggac gacgcgaatt ttctcaggtt ccggcaagtc
      361 ctggacatcg gagacgacat actggttcgt gtgcgccggt atctcgacaa tcaggatttc
      421 acgttccaac aactggcggg caaagtctat acggccattc gcgcgatttt cagaatgttt
      481 atgttgagcg caggcgcggg cttaatcgcc gccgtcgtgg ccgccacgct gtatgtcctc
      541 aacgtgttgc agcccagcta tgagatcttg caggccagga cgtggaaccc gttcggtgag
      601 caatttatcc tcacgaaaga cgattgggag tttacgcttc ctttttcgac cggggctttg
      661 caggtcatca ttctgatctg gtttatcgtc acggcggcgt tactgctcat gtatgggcga
      721 cgcatttacc tgcgtttcgg agacgtggac gactaagcac aacaagcagg ggcgagacga
      781 agaatcgtta gcggatggtg gagttactct atggcgtatt tcgcgatgca gtcccaggtg
      841 ctttttcacg acacgatggc ctatggctca caccatcata tggctaactt caagtttcag
      901 aacatcgcgc gtgaaaccat cctgtttgaa accaataccg aggggcagaa aagctggcag
      961 gagcagttga aggatattct cgtgctgacg cgcgaggcgt atagcctcaa cctcgcgccg
     1021 gtcggcctcg gcgggaaagt ggcgattctg atgacttatg aagagccgac gctgtgtacc
     1081 gtgcgcctct gtttccgggt catagaccaa cgcggagagc ctgtgtcgtg cggcttccag
     1141 accatgatcc tgttgcacaa ggatacgcac gagatggtgc ctgcgccgcc tctgctcaca
     1201 cactttctga cgaccgaaag taaggtcagc ctgcttgaag aactcaaaaa tccctcgttc
     1261 gccgaaagag tcgcaggcgg cagcagagaa ctcaagaacg tcttccccga atcaattcgc
     1321 cgtttgggcg caagcgtcgc caactcaccg cgcgagaagg cttatcccaa aatcctcgac
     1381 gaatcgcaaa atgaatacag cctttgatga aacgcaaaag aaatacaccc tctgaggatg
     1441 tgaaaggcta tgagaagtat ctcctccagc acttcaaaaa ccgatccgaa agtgaccttc
     1501 acttttcccg gccaaggttc gtactctcac acactgctgc gtgaactcta caccgcttac
     1561 ccgcagacca cgccctattt ccggcaggca gatgcgatca cgcgggaact tcttaaagcc
     1621 gacttccttc ctctggtgac ggctgcttcc gccgccgaac acgacgagcg attggagggc
     1681 tgtcccgatc tcgatcaggt tggaatctat ctggcagagg tgttgattgc cggaatctta
     1741 atcgagtcgg gcgtcaagcc gtcgctgctc gtgggtcaca gtttcggcga actggcggca
     1801 ctggcgacgg ctggcgtcta ttcgatagaa accggattga gaatcgtctg ccgacgcgtt
     1861 ttggcgttgc aatccgcagg cgcgtccggc ggcatggccg cgctctcgtg cgatctcgaa
     1921 cgcgccacac aattccttgc ggagtcggga agcaactcca tcgaaatatc ggttattaac
     1981 catccgcgcc agaccgtctt gtccggcaaa ctttcggagt tggaatcgtt gcgccccgtc
     2041 gtgaatcgtc agggtgtgtc gctgacgatt ctcaagagcc gctatccgta ccattcatca
     2101 ctactggcgg gcgcggtcga accgttccgc gctgccctcg acgagttcga ctacagcccg
     2161 gcagagattc cggtctacct cggcctcgaa ggcatgctct attcgccggg ctttgatctg
     2221 ccgcaaatac tctcgtcgca gttcatacgc aaactccatt tcagcaatgt cgttaaggtt
     2281 ctctatgaaa gcggctatcg aacgtttatc gaatgcgggg cgggggagat cgttacaaaa
     2341 ctgatcgggc agaatttggc gcaggtggcg agcggcatcg tggcagtgcc gtcggcaccg
     2401 caggagggaa gtctggacga agaattgcgc aagttgtttg cgcgtggctt cgggaaggag
     2461 gccgccacgc ttgcagcaag tgagacgcac gacgcgacgc ggagcagtca gcctccggcg
     2521 cagttgttgg agagcatgtc tctggtcgtg caaagcatgt ctcaactcgt cgagcacact
     2581 tctcgactga tcgagcaagt ctcgacttcg ctcgttacgg caaatctgcc cgtcgctgcg
     2641 cgggaggaaa atatttcccc tgccagccgc gagagtgtgg cgcagcctga cccggtccaa
     2701 ccggagactc ttcctgcgga cgctgccttc gtgcccgttc ctccgccccg gggaaacggc
     2761 tcgtcgcctg aatcgagcca tttggtaacc catgcgtctg gctctaacgg caacaatggg
     2821 tcgggcgcgc agccggagcg gaagcgcgaa ctggaagcgg cgttcgcccg cgaagaatgc
     2881 gcggcaacgc cgattgcagt ggtatctctg ggctgtgttc tgccgggcgc gaatgacccg
     2941 gagcagtatt ggtcgaacat cctgaacggc gtgtccggga tcaccaatct cgccgacatc
     3001 gaccccactg ccgccgaaga cttcctcctc acaaacgaag gcgacggaat aaaggtcgtc
     3061 cccgacaaga cttatactct gttgcacggc agcatcgttg acatccctta cgatgccgcg
     3121 ctgctttcga aggcttacag cagagagcag ttcgacgttc tcaccaaagg acaaaaaatc
     3181 ctcgcgctcg cagtggcgca gagtctggct cgcctcaagg tcaaaattga tccgtcggcc
     3241 tccgtcaaaa tgcagtgcat cctcggcgcg accgccgacg gctcgaagga gtttgacgag
     3301 gcgttgtttg tggaaagtct ctcgatgcac ttaggcgcgc tcgacgaacc ggagagcctg
     3361 cgtcgtgctt tcgccgacac cctcgaagag atcacgggct accgtaacgg cgatacgaaa
     3421 aaactgacac agcataagat ttacgcgagc gtgatcgagc ggctcatggg cagcgggctg
     3481 aaaacctacg tggtggacac ggcctgttcg tcgtctctct attcgaccta tctcggcatg
     3541 agagctttgc agaaccgcga gtgcgacatg gtgctcgcgg gcggcgtctt cgcgccggga
     3601 ccggccaaca atactctctt cgcgcagttc aagggattga cccaccgtga gagccgcccc
     3661 ttcgacgcag acgcggacgg cgtggtcttc agcgacggcg caggcatcgt catcctcaag
     3721 cgtctgcccg acgcgctcgc cgacggcgac cgcttcctcg ccgtgatcag ggctatcggt
     3781 ttgtcgagcg acggcaaaag cccctccatc aacgtgccgc aggtgaaagg gcagggcttg
     3841 gcgatccgtc atgcctacaa agattccgcc attgacgtgg acacgattca atacgtcgaa
     3901 gctcacgcca ccgcgactct ggtgggagat gcggttgagt tcaacgcctt gaaagatacg
     3961 atgcagcgca gcccggcctc cccgccggtc gaactcggaa gcgtcaaggc tctggtcggg
     4021 catacgggct gggcggcggg catggcgtcg ctcatcaaaa tctgcaaggc gtttgaggcg
     4081 agagtgatcc cgaagcaata caactacgtg aagccaagcc cggagatcga tctggctcat
     4141 tcacagttta cgatcacaga gacttcgcac ccgtggactg agaacgttgc gccctatccg
     4201 cgccgcgcgg cgatcaacgg cttcggtttc ggcggcacga acgctcattt gattctggaa
     4261 gattttgacg aagcctatca tcgaaacctc tgcgccaatt tggacgtgaa ggagaaagtc
     4321 cccgtcgagt tggccgtcat cagcatcggc agcctgtttc ccggcacaga cggcgcgttg
     4381 gccgcagaag acccgtccac ggagtcgcgc ttccggcggg acgcgctgcg tctaccggcc
     4441 aagaagatgc tgctccctga cgtgaccgag cacatggacg ccagccagta tctcgcagcc
     4501 ctcgccgccg agaaggcatt cgcgacgatg cccgaaggtt ggacgcgatt caaaaatgcg
     4561 atgggcgtgg tgctggggct gacgagcaag acggaacgcg ggatgcgtgc caacgaacga
     4621 gttttcgtag accgcctgcg gcgacaggtc acgcagcgcg gcgggagcgg caacctctcg
     4681 aacgccgacc tcacacgcat tttagagaaa ctggtcgagt cgattcgcgc ccgcaatgtc
     4741 ccttccggtc cttacaccct gccggggctg atgcccaatg tgacggcggg cagaatcgcc
     4801 aacatgttcg acctcaacgg acccaacatc gtcgtcgata tgggcgacga ttcgttgctg
     4861 caagccctgt tcgtggcgcg tcaacttctc gcacacgacg cctgcacaat cgttctggcc
     4921 ggaggcgtca acgcgaacaa cgccgccgat gcgagcgaag cggaggccgt cttcctgctg
     4981 gctctgacga cgcccgaaat agcgcgccgc gaggaactgt cgatcctgag cgtcatcagt
     5041 ttctccgggt cggaagaggg aactcatgcc ggggagaggc cggtttccgt gtcgcatcca
     5101 gccctcaatt acaaaggggc gcagggcgcg ccggagatgt tgaaggccat ccggcaagca
     5161 cgcgaaaaga actccctttg cgagttcagg cagccgggtg agggtgcgct ggcgggcagg
     5221 cgtctgatct tcgcctcgcc tgcttcgcac gcgcctgcaa agacggcgca gccgaccgag
     5281 acgcagagag acgcctcacg cgcctacgcc ttcgtgcagg gcacgccgat tcgttactac
     5341 acgccgcaac tggctccggc caaagctacc ggcgacgcga agagcttgca ggagcgcaaa
     5401 atcctgtttc tggttgacca gcccgaacaa tggttgacgc tcgaaaactc cggcgcgctc
     5461 gccgcgctcg actatcacgt ggtctgcccg tccggtgtcc gcgtggcgaa gtctctgccg
     5521 gttgatctca catccgaaga gagcattgag agttctttga gcggcctcgc cgggttggag
     5581 ttcgatacgc tgatcgcggt caagactctg gccgggcaga cgaaagagac gcttctcctc
     5641 aacaactcgg agggcgaact tcgctggctc gatctgttgt tcgcggtctg ccgacagagt
     5701 tacgagagga ttcaaagaga gtgcatcccg gtcgtcgccg tgtgtcaggg cgcgtatcag
     5761 aacggggagc ttgatccgtt tacgggtctg gccgccggat ttatgaagtc tctggcgagg
     5821 gaactcggcg agaatgtctg ccgcgccatc aacacggacg aagtcaattt ctacaagacg
     5881 ctgcggcagg tggagattga actggggcag gcgggagagg acgtagaggt ctgttacaga
     5941 gaagggaacc ggagcgtctt tacgctcgcg ccggtcgaga agctggcgaa ggatgagcag
     6001 ccacaactcg atgccgactc ggtggtgatc gctacgggcg gtgcgcgcgg cgtgacggcg
     6061 gtgctggccg aagagttgct ggaaaaattc ggctgccgcg tgatcgcgct gggacgaagc
     6121 gatccatcct caatgccgga agaagtgcgg acgatggatg agcaggcttt caaggagtac
     6181 gagccgcagt tctacaagga tgagctggcg cgaaataagg gcaagaagat gggcgaattg
     6241 aaacgggagt atctcgccta tcgcgccgcc aacgaggtgt gccaggtcac cagacgcttg
     6301 caggccatct ccggaaagta cgaataccgg agcgttgaca tcacgagcga aaaggccatc
     6361 gaagaggtgg tcgcggaaac atatcgaaag tatggtcggg tcgatctggt gctgcacggc
     6421 gcgggcgtgc aagtctcgaa ggcgctcacc aggaaatctc tcacggactt ccgcaagatc
     6481 gtcgccacca aattgcgcgg cctgagcaat ctgtataagg catgccggaa gtatggcgag
     6541 ggacgccgca ttcatttcca cattctgacc tctgtgttca gctacatggg gaacgacggc
     6601 cagccggatt acggcgcagc caacgaggcg atgagccgca tggccgcctg catgaactcg
     6661 ccggagaacg gcaaccactg gtcagctatg gcgtggctcg gttgggctgg catcggcatg
     6721 acacgcgact ccgagtttgc cgcgctcgcc gcctcaagac gcttgcgagg cgtgacgaaa
     6781 gaggaaggac aacagatctt ctccgagttg atgcggggca cgcccgccac gcccatcaac
     6841 atcctcctcg ccgacggcga gatcgaatac tacaaggtgg cgattcaaac ttcgtcgccg
     6901 gaagcgtcgc cgcgcgcgtc gcaagcgtca gcgcaagccg cgccgccagc cgtctctcag
     6961 gcggtcaagg ccaagccgga ctttcatgtg atggagcggg aaatctcggt cgaaagctct
     7021 ccgtatctgc tcaaccatct catcgacgga gtgccgaccc tgccgggcgc gctcgtcatc
     7081 tcgctctttg ccgaagcggc gcaacaactt cgacccgaac tgaagatcaa ctcctttgaa
     7141 aaagcgttct ttcatcgctt catcaaagtc taccagaacc ggaagacgcg catccgcgtc
     7201 gaggcgcgca tcgtttccga ggatgagcgg cagacggtcg tgcaggtcag gattctgtcc
     7261 gacttcgttc acagcagcgg ggtcgtgctg caaaaggaga tcctccaaca tgaaatcctg
     7321 atccgcatgt cgtccgccgc gctgacctct ccggcgcgcg tccacccgaa cggactcgaa
     7381 gggcgacgcc tgctcgaccc ttacgtgatg gatggttctc cggtctgcct gagcggcccc
     7441 ttcaacgcca tggagaacat catcgtcggc ggagcgcagc ggaaggccga gttcaaactc
     7501 gccgacttca acggcgcaag cccggcatac aaatcggagt ggtcgaagat tattctgatg
     7561 gattcgctct ggagattcgg cgtgattcac ctcgcgcccg acgactcctc gcccgtcttt
     7621 gttcccgaag agtgcagggt catgaaggtc tatttcgact tctccgactt cgacgcctcg
     7681 acgctcgccg ggaagttgac tttcagcggc gtgaacccgc gtctggacgg cgaccggctc
     7741 accatcggtc cggtgactgc ggctgacggc aacggcaatc cgctgatcat cgtggaagac
     7801 ggaatctgcc gtcgatacgg aaaagtaagc aatggcaacg gcacctcaca ctaagacgga
     7861 agcgactgcg gcgcgcttcg acgactgctt tgtcggctgg ctcgacgtgt gtgaattgca
     7921 ggacagggaa tatacaagcc acctgtctgt ggacgagcgg gcgaagtact tgagtcttcg
     7981 cggcgagcga cggaaaagcg agtggctggc gggtcgtctg gcagccaagc acctcttcct
     8041 gaactggctg gaaccggcgc gggaaattcg gagcgggcaa tggaatccgg gtctgtcgcg
     8101 gctttccggc gaggctctcg ccgcctattc gccgtggatg taccggaagg tcgaagtgct
     8161 ttcggatggc gagaagcccg gcctcgtctg gtgcgggcgg aagcgaaagg aaagcctctc
     8221 gatctcgcat gcgggcggaa tctcctgcgc gtccatagct ctggaaaagc ccgtcgccat
     8281 tgatgtcgag actgcggttg acaggcacgg cgcattctac cgaaacacct ttacggaagt
     8341 ggaaaagaac tgggtcgccc gtgtcggcgg cgacgacggg ttgagtagtg cgaactggtg
     8401 tttcacgctt ttgtggagct tgaaggaatc ggttttgaaa ttgggatggc tggacggggc
     8461 aagcatctgg aatctttcga ggatcgagat cgacggcttg cccggcctca accagcccgg
     8521 ctttaaccgt agagagccgt tctggcgcag ccgcaacctg agcgacgctt ttgtcgtctt
     8581 cacggcaaga gtcaaagaac ctcgccgcgc gatgcgggta caggtagcgg tgaccggtac
     8641 acgcaacttc gttttaaccg ttatgaatcc tctgattgga gcagtcaatt gagcacagca
     8701 tacacactcc ccgtcgcaaa cgatcttaac agtgccgtcc gcgaaatctt tgcacaggtg
     8761 acgcgctacc cgctggaagt cttagacccg gcatccggtc tggaagaaga cctcggcatc
     8821 gactcggtga agctgggcga ggtgttcgcg gtcttgcgcg agaagtacgg cctgccggag
     8881 aagctcgatt ttccacgcga aaagctcaag accatcggcg gcatcgcgga agccctgcaa
     8941 gtctacctgc gcgatgcatc gcctcccgcg cacgccggag gaaacggcgg cagtaacggc
     9001 tcggcgggac tcgcgcagcc gcactccgga aacgggaacg gcaacggcaa cgggaacagc
     9061 ccggtggatg ccgcgacgct tcaagccggt gtgcttgaaa tcttcgcgca ggtgacgcgc
     9121 tacccgctgg aagtcttaga cccggcggct ggtttggaag aagacctcgg cattgattcg
     9181 gtgaagttgg gcgaggtgtt cgcggtcttg cgcgagaagt acggcttgcc cgaaaagttg
     9241 gacatcccgc gcgagaagct gaagaccatc ggcggcatcg cagagtcgct gcacgaatac
     9301 gtctcgcaca gcgtcgctcc gccgcaggtt gtgaagccgc cgagtccgcc gccctctccg
     9361 gctgtgatct cgggcggcaa cggcgcgcac ctgtcgtcca cgatagccga gtcgttcgcc
     9421 tctctgatgc cggagcgcaa gccctttgcc ggaaaggtcg cgctgctgac cggctccggg
     9481 cgcgggctgg gcaaggatgt cgcctgttat ctggccgaac ttggcgcgtc cgtgatcgtc
     9541 aactctttcc actcccggca ggaaggcgag cgcacggtcg aagagatcaa agcggcgggc
     9601 ggcgacgcga tccacgcgtg gggttcggtc gcaaatcccg aacacgtgaa gaccatcttc
     9661 gacaagatcg aggagacgca cggcgtgctg gacttcttta tcagcaacgc ttcaaacggc
     9721 atgctcgcgc gcctgaaaga gatcaccacg gagcactggg aaaaagcctt ccgcaccaac
     9781 gtcgtcggcc tgcaccagac atcgctcaag gccgtggaac tgatgaagag acgcggcggc
     9841 ggcaagatca tcacgctctc cactccggcc tcgcacggct atgtggacta cttcggctgc
     9901 atgggcgcgg tgaaggccgc cgtcgagtct ctgacgaaga gcatggcgat tgaattcgcc
     9961 gccgacaata ttcaggtcaa ctgtgtctcg ccgggtccgg tctatggcga cctgctgaac
    10021 aagtggcctg agagcgagcg tctgatccgg cagtgggaaa acaacacggc ctactcgcgg
    10081 ctctgtgagg cgcgcgacgt gtcccatttc atcgcttacc tgctcagcga gccggtgaag
    10141 ctgttcacgg gcagcgtcct tatcatggac ggaggaattt cgtcgcaagg ttggtaaatg
    10201 gttggtagag agttcatgtc gctggcaata cacacgaacg gaaatctctc gcggttcaac
    10261 cgactcaaag gccggttgct cggtctgtct ccgaaggaag cctccttttc gcggcgcgcg
    10321 tttccgggct gcggttcagg cagccgcgag catctggaga gcgtcatccg aacctttatc
    10381 gaaggctaca atctggccgt tcgtgagtcc gacccggttc gtttagctca aaggctggac
    10441 tcgacgtttg cgcctgcctt cgtcgggttc gcatacgaag gagtgggact ctatttcggc
    10501 ctcgttgacc tgctgcttcc acgctccggg agccgtctcg acgccttcac gcactccgcc
    10561 ggagagcgac acgatttcat catttcggtc ggcgcgggct ttgccgtcgc gcgtgtgccg
    10621 ctcggcctgc gccgtctgga gtcctatcaa aaatcgctcg acccgatgac cgcctggtgt
    10681 ctggccgacg gctacggatt ccacgagggc ttctttcact ggaagcgttt cgttgacggg
    10741 cgacagccag cccccgcatc gctcagcttg cagaaccgca ggctgttcga tgccggagtc
    10801 ggtcgtgcga tgtggtgggt ttacggcgcg gatgcggcct ctatcgcggc ggccatctca
    10861 cgcttcgacg ccgaacggcg gcatgagatg tgggctggta tcggcaccgc tctggcctat
    10921 gccggtggcg tgacgccgga ggccgcttcg ctcttacacg acctcgccgg gtcgtatcaa
    10981 tccgacctgt tgagcggcat tccgttcgcg gcgcacatgc gcgataaggg gcggaacccg
    11041 gcggcctgga ctgatgaagt ttgcatgggg ttgttgcgga tgtcggtcgg agaaacgtca
    11101 gaaattattg ttaccgaatt ggccgcctat cttgataact ggcaaggctc ggaaccggac
    11161 aagtggagcg gttgttacgt ggctttgcgc gagcgtgtgc ggcagcgtct gttgaaacgg
    11221 cgcgctacac cctccgctaa ctttgctttg acgaatgggg attgagagga gaagtatgac
    11281 caccaatact gattacgatg tcattatttt aggaaccggc atcggaggct cgatgctcgg
    11341 agccatcctt gccaagcata atctgaacgt gctgatgctg gatgccgaga cgcatccccg
    11401 tttcgcgatt ggggaagcga ccactcccga taccaacttc cggctcaagc tgttgtcgca
    11461 caaatacgac ctgccggaga tcgcacacct cagtgcgttt catcccacgc gcgacttcat
    11521 cagtccggcg tgcggagtga agcgcgcctt cagttttctc taccagcgcg aaggcaagga
    11581 tcaattgccc atggagacac atcaataccc gacgctcgcg ccgccgatgg gacccgattg
    11641 ccatttcttc cggcaggaca cggacgccta catgatggcc gtcgccgtca actacggcgc
    11701 gaaggttcgc cagcagacgc gcatcaagga tttcgacata caggaggatc acgtttcgct
    11761 gacgagcgag aagggcgaaa cgttcacggc caagtatctg gttgacggga cgggcatgaa
    11821 gtctgtgctc gcgcataagt tcgaactgcg taaaccggcg gagactttcc gcaccaacac
    11881 gcgagccatc ttcacgcaca tggtaggcgt caagctctac gatcaggtcg ggtcgtcgca
    11941 acagacgcac ggccacaagt atccgctctc gcagagcact ctgcaccacg tcttcgaggg
    12001 cggctggtgc tggatcattc ctttcaacaa tcatcaggac agcacgaacc ctctatgcag
    12061 cgtcgggctg gtgctcaatc gtgagattca cccggaaacc ggcatgaatc cggaggagga
    12121 gttctggtct ttcgtgaaga agtttcccga tatggtgaga cagtttgaag gcgcgcaggc
    12181 ggtgcgcggc tgggtcggca cggggcgact ccagtacggc gcgaccagca tcacaggcca
    12241 ccgctactca ctgctctcgc acgccgggtt ctttatcgat cctctgtact ccaccggcct
    12301 tgccctgacg acggcaatgg ttgacctgct cggcgggcag ttgctcaggg ctttcaagaa
    12361 taacgatttt tcggtcgaga gcttcgagca tctgaacaac tttttcgata gcaacatcgg
    12421 ttatgcagac gagatggtga gcagttcctt cgtgtcgttt cgggatttcg aactgtggga
    12481 cgcatggttc cgcgtgtggg tggtcggcct ctttatcggc acgtgtctga acgccagcct
    12541 gtatctgaaa tatatcgaat cgaaagacaa gcgcgtgctc gaccagtcgg ggaaggaacc
    12601 ttactccgtg ctgctcggcg gcaagtttcc ggagtttcag aaactctacc gggaggcgct
    12661 cgccgaaatg gaccgggtga aggccggaca ggttgacccc aaagaggcgg cgcagaagat
    12721 acgcggctta ttcaagggac tcgactacgt tcccacttac tggcgctggc acgaagcggt
    12781 tgtgcgtacg acgcccgcct tcacgatctg gggcatgacg aagatgtatt tctggtttct
    12841 gttctacgct ccgaagccga tccgcgaagc gctgttcggt tggagttcgt tcaccgccta
    12901 caaatatatc ctcggctcca tactggataa caacggggca tcgaagcgtc gcaaacgaag
    12961 ctacatcagg gacgtgttca agtcgtggaa tctcgattgg cttccggcga agtcgcacga
    13021 gtaggagcgg cagcggcggt cgccccgcga gcggaggtta tgttcggcgc gagcgtcacc
    13081 ccctcgcgta acgaacgcga cgcgccgcag cgtcgggttt acggacgacg gccatcgttg
    13141 ccttgagttg gttcagacga atcggacacg cgggaacaat cggttttgcg tccagcccaa
    13201 cacctcgctc gaagagttgc agcacactcc aatggaggag ttagtcgatg ttcaatatcc
    13261 ccctactgat cgccgcagga gtcgcgctcg tcgcaatctc actgcacatt tacaccttcg
    13321 aagtgtggat ttggcctaag ctcaaagacg actgttttcc cagtttcccc ttcggcggtc
    13381 cagacgtggt gaagggcttc taccggacag tgtggcactt cttcacggtc aatctcctga
    13441 tgaccatcat cgtcaatttc atggtgggca tcgggacgct catcccctac ggcctcctgc
    13501 tcatctacta cctgatcgtc ttctggattc tcatctttat agagatctgg ctcatcggcg
    13561 cgttgtctct gcgccccggc cagtcctatc tcaagacgat gttgaaggcg ttccagtggg
    13621 tcttcgttct cctcatgatc atcttcatgt actggggcac ccggaaatag ttgagcgcgc
    13681 cggaagagtt acggggcaac tgcccctcgc gaggttaagc atgaaaagat tatttgcgtg
    13741 gatcgattca gtggttaata aactggcgac gaacttcacg ctctggatcg tcggcggtat
    13801 cgtcgcgctc atcgcggtgg cctacacggc ctatgccacg gtctttgtgc ggctgccgga
    13861 gccgcccgtc tatgcgaagt atgtggagct tcaaccggaa gaggaagagg cggggcgcaa
    13921 gcatggatgg aacgcagacc gcaggcaacg ttattaccag accagccagg gcagcctcgt
    13981 aatgccttac gcctggttcc gcgcggtgga gtggcgcacg agcacggaga tgttcgcgtc
    14041 tccgcaggtg cagaccagat acgggctgct gccggataac gaccccgtct acaaccggga
    14101 ccagatgccg gtcggcctcg tgaagcacat cgtgccggac caatatgtcg ataacctcgg
    14161 cgaaggggag aaagagtggg cgagtatctc ctgcgccgcg tgtcatacgg ggcagatcct
    14221 ctacaaaggc accgcgatgc gcatcgacgg cggccagagt ttctggaact tcgataaatg
    14281 gtcgggcgac atggtcttct ccctgatgct gacttccgcc tcgccgcaaa ggttcgaacg
    14341 cttctgtgcc cgcgtctacg gacgccgacc gggcgaaaaa tgtgcgtcca acgaagtgac
    14401 gacgctgcgc gcacagatga agaggtactt caacagcgac ctcatcaagt cggccatcga
    14461 ggcgctcatc aaccacacct atccgaccac ggagggcttc acccgcacgt ccgcgctcgg
    14521 gcgcggagtc aacggcgagt tcggctcgct cgatccttgt gaggggccgc tcgaccgtag
    14581 ctgtaaccgg aacgtgaacc ggaacacggg acccgtgagt ttcccgccgc tgtggtacac
    14641 gcacgaatac gattgggttc aatctacgac ggcgatcagc cagccgctcg gtcgcaacgt
    14701 gactgaagcg tggggcgtca gtatgcgcgt cgaactgaaa gacccatcca cacgcttcgc
    14761 ctcgaccgcc aacgtcgaag acatgttctg gatggagact ctgatctcga tcctgacagc
    14821 cccgaagtgg ccggaggaca tcctcggcaa gatcgactgg gagcgtgtga agcggggcaa
    14881 gtatctctat cacgatgcgg tgtggaacaa ggctctgccc gccgcgcagg ccgaactgca
    14941 accagacccg gccagcctga tcggcgcgcc gaacccgaac cgcccgacga ccggcttatg
    15001 cgcgcgctgc cacgctccgg ccttcgaggc acagcccgcg ttgacggcag ccctcgcaca
    15061 ggctcccggc accaatccca accagtacga aaaacggttt cttcaactcc cgctctatcg
    15121 tcaggaggtg atgggcacgg atgacgacga tgcggtgcaa ttcaacgaac gccgcgtgca
    15181 tcccggcgtc ctcaaagagt tgttcaagca ggagcagttc gatgatgaag gccggatcgg
    15241 gatcggcgac atgctgaccg tgaccatcaa cggcattctc gataagtggt tcaaggaaca
    15301 taacatcggc gaaccttgca agacgatctt gcagggctat cgcccgaacc agtttcgcgc
    15361 gccgctcggc tatcccgcgc gtcccctcga cggttactgg acaaccggac ctttcctgca
    15421 caacgggtca atccggacta tgtacgaact gttgtctccg gcggcggagc gagccgagaa
    15481 gttctggatc ggatcgaggg agtttgatcc ggtctatttg ggttacaagg acgagccggt
    15541 ggaaggagct ttcagcttcg acatctcgca tcaaggcaac tccaacgccg ggcatgaatt
    15601 ccgcgatgcg ccgccgaata ccgtcggggt cattggtccg gcgctgacac acgagcagcg
    15661 gctcgacctg atcgagtacc tcaaggtgat cagcaccgtg ccggagttgc tcgataacga
    15721 cccggcgacc agagagcgat tggccgagcg aaatggcatg ctcgatgctc tgaccccttt
    15781 ctatgaaaag aacgcgggtt gggtgaccta cgggaagaac aagccggaga gcgagggcgg
    15841 ctacagccgg atggacttct gcaagaacat cgaggcggcg gccatgcgcc agtcctatgc
    15901 ggctccgtcg gcgtcgccct cgcccgcgcc gtctgcgtct cccgctgcga cgccgtatcg
    15961 aaagaaaagg tagcttcgta tgagccgctt gaacgacatg agaatatcgg ccatcggcgc
    16021 gccgccgcag ggtgagctgc atgagacgcg gctggatatt ccgttcacga ttatcaacat
    16081 caccacgacc atcgcggcgt gcgtgctgcc tttctatttc tactctcgct cgtcaatcgc
    16141 cgcttttctg atcttctaca cgatcacggg catgggattg acgctcggtt atcaccgcct
    16201 gtttgcgcac cgcgccttca aggtgccgcg ctggctggaa cgactgatcg cgctctgcgg
    16261 ctatctggcg attcagcgtg gacctatctt ctgggtggcg atgcaccgcc tgcatcatcg
    16321 ctacagcgac attccggggc gcgacccgca cacgccccgc gaaggttggc tgcacgttca
    16381 tttcggttgg caccagcgtc gccgccgtga tgtctgggag agcgagatat acaggcgata
    16441 tacgccagac ctcgtcaacg acaggttcta cctgtttctg gataacgagc ggcgcgacta
    16501 cttcacctat ttcggcatcc tgttcggctc ctatctcgtg ggcggactcg tcggcgcgtt
    16561 cgggacgacg ggtttccaat tcgatcacta caacgccgtc tgcttcctcg tctgggtcgg
    16621 gttgttgaac cgcgtcgccg tgctgcatgc gttcggcttc atcaactccg tctgccatga
    16681 actcggaacg cgtcctttca agacgcgcgg taccgacacc tccgtcaata acgtgatcgt
    16741 ctcgctgctc atcttcgggg aaggatggca caacaatcac cacgcgcatc cgaagtctgc
    16801 ccgtcagggt ctgcgctggt atcaatggga ccccgcctgg tacggcatct gggtgctgaa
    16861 aacgctgggg ctggcggaga aagttcacgt gccgagcagc gaggtcatca tcaggtcatc
    16921 gaggagctat gtcgtccctg cgggtgaagg gctggcgcgc gcagaggccg tcggcggctc
    16981 gtatgaacga aaaacagcaa ggggcaaagt tgagccgttg gaagacgcct gacatgaaaa
    17041 tctactttgg taaggtttac ggtgcgcaca tggataaact caagacgatc tggttgccga
    17101 gagggaagag ggtatacgtc tggcagggac tcatctcgac cgaccagcgt gcgtatgacc
    17161 agctaacggc aatcgctccg aataatgccg gggggcgacc tgccagaagc ggcaggcaga
    17221 acgcccttct attcaaagaa ctcgaagaga acaaccgttg ggcgcgtctg ggagccaaca
    17281 tctacttcgg atggtttacg ctgcttctca tcatcaatgt gatcgctgcg atctggctct
    17341 tcacctacag aggcgtcagg ccgccatacg cgcgcctggt ctttctcctc atcatcggaa
    17401 tgaatctgac gggaaccgtg gtcaccttct ttatacgcaa acacatgctc gattgcgacc
    17461 ggcgcataaa aggagtgctt gaggaactca gacaagaacc cgcagccatc ggcgcgtatg
    17521 acgagccgca gtcgcccgtg ccgcgtcagg tcgtcaacgt gatgttcgcc ttcacgggtg
    17581 tcatcttctt catgttgttt ctcttttgga tgatcctgga aatttggccc catgttttcc
    17641 tcacctagct gccggatggc cgtctgtatt cagaggacga actccaaccc gtgcgcggcg
    17701 tcggcctgaa agcaaaagcc agtcctaacg gagcctcaga cgaagactat gaaacgggac
    17761 gcacaattag aactcaataa ggaatatccc gcgcccgacg agcgtgagat cacggagaag
    17821 ttgatcgtcc tgctcaaagg gattatcgag cagcgatacc tcacgggcgt gacgtttcgt
    17881 gatgtgcacg tgaagggaca cgcgggagtg agagcggagt ttatcgtcga gccgaatctg
    17941 ccggaagagt ttcgcgtggg cgtcttcaga gaggcgcgca cgttccccgc gtggatcagg
    18001 ttctccaact ccagccaaga ccccgcggtt gacatcaaag acgacatacg cggcttcgcg
    18061 ctcaagctca tgggcgtcga aggtgagaag ttgatcgaga gccagaaaga cgcgaccacg
    18121 caggacttta tttttctgag caccaatctg tttctcacca aagatgcgaa ggacttttac
    18181 gaactcgtcg agtgcggcgc gctcaacaag aaaaagtcgc tttcggatta cctcggcatc
    18241 ctcggttatt tcgccaggca ccctgacgtg ggagtgaaac tgctggcggc tttgcgcaag
    18301 tttccgcacc tgctggagat ggaatggttc agcgcaactc cttatctctt cggcgagcgt
    18361 gcggtgaagt atgcgctcag gccgtggctc aagacgaaaa cggcgttgcc gcgaaagccg
    18421 ggcaacaact ttttgcgcga acgcctgacg gagcatctgg cgcgcgaagg cacgggcttc
    18481 gatttcatgg tgcaatttca actcgaccct taccgcgagc cgatagaaaa ttctctcgtt
    18541 ccgtggaagg aagagaacac tccgtttcac aaggtcgcca gcatcaacat cccccgtcaa
    18601 aagatcgact cgccggaaca gatgtcgttc gccgaaaact tgtcattcaa tccctggcac
    18661 tctctgccgg agcacaggcc gctcggtagc gcgaaccgtg tgcgcaagga ggtgtacata
    18721 gctatttctg agtacaggcg ctcaagaaat ggcgtcccga atacggagcc tactcccgat
    18781 gaaagtttct gaatgtgccg cactgcccca ccgccgcacg gttcctccat gccggtttgg
    18841 aagtaaagcc gagacgcgcg agcgcgcggg tggcgaacta aagagcgtgc ttcctaaacc
    18901 gttcaattca gagaggagta gattcgaggt aagaccgaca tgttagaaaa aattgtgaac
    18961 ttcctgaaat ccctcaaact gtggaagaag attgcgctgg gtgttctgct cctgctggtg
    19021 gtgggcgtgc ccctcgccta ttacctctac aaccgcgacc gcgcgcaacc gttgaaacca
    19081 tccgccgaag tcctgtacct gaacaacgag gtgtggaccg aggccgagcg tcagcgttat
    19141 taccatctct cgcagggcgc gcagatcatg ccctacgatt ggctcatcgc cctcgaac
//
LOCUS       DASWNS010000043         5379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_417093, whole genome shotgun sequence.
ACCESSION   DASWNS010000043 DASWNS010000000
VERSION     DASWNS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417093"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VGB76_01535"
     CDS             <1..251
                     /locus_tag="VGB76_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097609.1"
                     /translation="NDSFSDGLLDFPHYTRPPEFRGQCVPEVLLGGNHAEIALWRRRR
                     ALRKTQRNRPDLLTRASLSEAERAWLAEAEEPENFRDE"
     gene            343..747
                     /gene="rplS"
                     /locus_tag="VGB76_01540"
     CDS             343..747
                     /gene="rplS"
                     /locus_tag="VGB76_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY0097610.1"
                     /translation="MNRLDAVEKTQMNKTLPDFQPGDTVRVHVRVKESETKERLQVFE
                     GLVIARKGGGVRETITVRKISFGIGVERIFPLYATIVKDIEVVKRGRVRRAKLYYLRN
                     LRGKAARIRERDTRPTSSGQVQAAAAAARKTK"
     gene            809..1468
                     /locus_tag="VGB76_01545"
     CDS             809..1468
                     /locus_tag="VGB76_01545"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY0097611.1"
                     /translation="MSLVTRHSSLMARQKRASEICTAFEDEARAGGHRMVAGVDEVGR
                     GALAGPVVAAAVILDPDTPLPEGLDDSKKLTALQRERIAEELKASALCYGVGLVPPEE
                     IDSTNILIATRRAMCHALGELKPCADFLLIDALQLREIALPQRAIIRGDSISASIAAA
                     SVIAKTYRDALMRDYDSVYPEYGFASHVGYGTRAHLEALRAHGACPIHRRSFRGVLPA
                     G"
     gene            1547..2680
                     /locus_tag="VGB76_01550"
     CDS             1547..2680
                     /locus_tag="VGB76_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0097612.1"
                     /translation="MHIAIDAHSVGTGLAGNETYITNLIEALAALDTAHRYTLYVTKR
                     AALERFAARWPHVSVRRTLPHTPFVRIPLTFPVELRRRPVDLLHVQFTAPPFAPCPVV
                     STIHDLAFEHLPETFNRRSWMQLRLTVRRTARTAAHIITPSEFSRRDLIETYNVAPER
                     VSVTLEAAAPHFRPASAEAITAVKRRYGINGDYVLAVGSIQPRKNLVRLVRAYSDLRR
                     SRSQAKLPRLVLVGKRAWLYGETLRAVEQSGTGQDVIFTGYVPERDLPALYTGALCFV
                     YPSYFEGFGLPPLEAMSCGAPVIAGDRTSLPEVVGDAGLLVNPFDTDAIGESLARLID
                     DEELRTRLRQRGLERAALFDWRDTARRTLQVYEQATGGKQSDE"
     gene            2771..3934
                     /locus_tag="VGB76_01555"
     CDS             2771..3934
                     /locus_tag="VGB76_01555"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEY0097613.1"
                     /translation="MRIAILGTRGVPASYGGFETFAEQLSTRLVARGHHVTVYCRSHY
                     VSPRQLEYRGVHLKVLPTFRHKYFDTIVHAFLSALHSTTKRYDAALICNAANAPFAPI
                     VRLGGTPVAINVDGLEHKRRKWNWLARRYYLLAERLSTVLPNETVTDARVIQDYYMAQ
                     YNHPTTMIAYGAEVARIPDRMAVRRWGVEPNRYVLYVSRLEPENNARLVIEAFKRVRT
                     SHKLVIVGDAPYAHEYIEELRASARRDRRIVFTSFVFGDAYRALQQNAYCYVHATEVG
                     GTHPALLEAMGYGNCVLTLATPENIEAVGEAAIPYSDEKDLEEKLQRVLRDGSLVNSY
                     RQRAQKRVSEHYDWERIVDLYENLFARMAGQREPHRAVAELVEAESNKEAVPR"
     gene            complement(4064..>5379)
                     /locus_tag="VGB76_01560"
     CDS             complement(4064..>5379)
                     /locus_tag="VGB76_01560"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0097614.1"
                     /translation="KSFKLANGLTVLLVEQHNLPVVAANLVVLSGSEANPADKPGLAS
                     FTADMLDEGTNKRSTLQIAEDVAAIGATLGTGSSSDASTVSVRTLKKNADAALELVAD
                     VALQPAFAAKELDRVRNTRLTQVLQQRDNPNVLASKVFNSEVYGTSHPYGFTELGTEE
                     SIKAITREEMAKFWQAGYVPENAALVVAGDLNESELRALAEKHFGKWAGKAGTVTRPE
                     VRTGAARRILLVDKPGAPQTALRIGMVGVPRANPDYVPIEVMNTGLGGLFSSRINMNL
                     REKNGYTYGAGSVFQFRRGPGPFFTATSVRTDVTAPAVREIFNEFERMRTTDVSAEEL
                     KIAKDSFARSLPGLFETTGQAAATIGQLFIYNLPLDYYSTLPGRIDAVTVADVRRVAG
                     KYLLPESMVIVAVGDRSKIEPELLKLNLGKVEARGLDGKATTPEK"
BASE COUNT          977 a         1744 c         1620 g         1038 t
ORIGIN      
        1 cgaacgattc tttctccgac gggctgttgg actttccgca ctacacccgc ccgccggagt
       61 ttcgcgggca gtgcgtaccc gaagttttgc tcggcggcaa ccatgccgag atcgcgctct
      121 ggcgcaggcg gcgagcgtta cgaaagacgc agcgcaaccg ccccgatctt ttaacgcgtg
      181 cctctctgag cgaggccgaa cgcgcgtggc tcgcggaagc agaggagccg gaaaattttc
      241 gtgacgagta acgagttggc gggcgaccga agtttgcagc ctgttctaac ttgtcacctg
      301 tcactcgtca cttgtcactt ttaatagctt ggagtgttag tcatgaaccg tttagatgct
      361 gtcgaaaaaa ctcagatgaa taagaccctc ccggactttc agccgggaga cacggtgcgc
      421 gtccatgtgc gcgtgaagga atcggagacg aaagagcgtt tgcaggtctt cgaaggactc
      481 gtcattgcgc gcaaaggcgg cggcgtgcgc gagacgatca ccgtgcgcaa gatcagtttc
      541 ggcatcggcg tcgaacgcat ctttccgctc tacgcgacga ttgtgaaaga tatcgaagtg
      601 gtcaaacgtg ggcgggtgcg ccgcgccaaa ctctattacc tgcgcaacct gcgcggcaaa
      661 gccgcacgca tccgcgagcg cgacacgcgc ccgaccagca gcggacaggt gcaggccgcc
      721 gccgctgccg cccgcaagac caagtaaaac agtgacgagt gacaagtgac gagtgacaag
      781 ttaaaactgc ttctctttat ctcggcactt gtcactcgtt actcgtcact cctctcttat
      841 ggcacgtcaa aagcgcgcat cggagatttg cacggcgttt gaggatgagg cgcgcgcggg
      901 cggtcatcgg atggtggcgg gcgtggatga ggtcgggcgc ggcgcgctgg ctggtccggt
      961 cgtcgctgcc gccgtcatcc tcgatccgga tacgcctctg ccggaaggct tggacgactc
     1021 gaagaagttg acggcgttgc agcgcgagcg catcgcggaa gaactcaaag cgtctgcgct
     1081 ctgttacggg gtcggcctcg tcccgccgga agagattgac agcaccaaca tcctcatcgc
     1141 cacgcgtcgc gccatgtgcc acgccctcgg cgaactcaaa ccctgcgccg acttcctcct
     1201 gatcgacgcg ctccaactga gagagatcgc cctcccgcaa cgcgccatca ttcgcggcga
     1261 ctcgatctcc gcttccatcg ccgccgcttc cgtcatcgcc aaaacttacc gcgacgcgct
     1321 catgcgcgac tacgattcag tctatcccga atacggtttc gcctcgcacg tcggctacgg
     1381 cacgcgcgcg cacctcgaag cgctgcgcgc acacggagcc tgccccatcc accggcgcag
     1441 ctttcgcggc gtgcttcccg ccggatgagc ctctggggta aactcgcgcg cgttaatctc
     1501 cctcctcaaa acagaacgcc agaacgcacg ggagcaactg aaccgcttgc acatcgcgat
     1561 tgacgcgcac tcggtcggca cggggttggc cggcaatgag acttacatca caaatttgat
     1621 cgaagcgttg gccgcgctcg acacggctca tcgttacacg ctctatgtga cgaagcgcgc
     1681 ggcgttggaa cgtttcgccg ctcgctggcc gcacgtctcc gtgcgccgca cgctgccgca
     1741 cacgcccttc gtgcgcatcc cgctcacctt cccggtcgag ttgcgacgcc gccccgtcga
     1801 tctgttgcac gtacagttca ccgcgccgcc cttcgcgccc tgtcccgtcg tctcgaccat
     1861 acacgatctc gccttcgagc atttgccgga gactttcaat cgccggagtt ggatgcaact
     1921 gcgactcacc gtgcgacgca cggcgcgcac cgccgcgcac atcatcacgc cttccgagtt
     1981 ttcgcgccgc gatctgatcg agacttacaa cgtcgcgccg gagcgtgtca gcgtcacgct
     2041 cgaagccgcc gcgccgcatt ttcgtcccgc gtccgccgaa gcgattactg ccgtgaaaag
     2101 aaggtacggc atcaatggcg actacgtgct cgcggtcggt tcgattcagc cgcgcaagaa
     2161 tctcgtcagg ctcgtgcgcg cttactcgga cttgcgccgc tcgcgttccc aagctaagct
     2221 gccacgactc gttttagtgg gtaaacgcgc gtggctttat ggcgaaacgc tgcgcgccgt
     2281 cgagcaatcc ggcaccggtc aggatgtgat ctttacaggc tacgtgcccg agcgagactt
     2341 gcccgcgctc tacacgggcg cgctctgctt cgtctatcct tcttactttg aggggttcgg
     2401 gttgccgccg ctcgaagcca tgagttgcgg cgcgcccgtc atcgcgggcg accggacgag
     2461 tttgccggaa gtggtcggcg acgcggggct gctcgtcaac ccttttgaca cggacgccat
     2521 cggcgagagc ctggcacgcc tcatcgacga cgaagagcta cgcacgcgtc tccgccagcg
     2581 cgggctggag cgcgccgcac tcttcgactg gcgcgacacc gcgcgccgga ctttgcaggt
     2641 ttacgaacaa gcgacaggag gaaagcaaag tgatgagtga tgagtgatag gagtgatgag
     2701 tcgaagcaaa tctcttttgt ctttgactca tcactcatca ctcatcactc atcactttcc
     2761 acttgcggcg atgcggatag cgattctagg aacacggggc gtgcccgcga gctacggggg
     2821 ctttgagact ttcgccgaac aactctcgac gcggctcgtc gcgcgcggtc atcacgtcac
     2881 agtctattgt cgttcgcact acgtctctcc gcgtcaactc gaataccgtg gcgtccacct
     2941 gaaagttctc cccaccttcc ggcataaata tttcgacacc atcgtccacg cctttctctc
     3001 cgcgctccac tcgacgacga agcgttacga cgccgcgctc atctgcaacg ccgccaacgc
     3061 gcccttcgct cccatcgtcc gcctcggcgg cacgcccgtc gccatcaacg tggacggact
     3121 cgaacacaaa cgccgcaagt ggaactggct cgcgcgccgt tactacctgc tcgccgaacg
     3181 gctctcgacc gttctgccga acgagactgt gaccgatgcg cgcgtgattc aagactatta
     3241 catggcgcaa tacaatcatc cgacgacgat gatcgcctac ggcgcggagg tggcgcgcat
     3301 tccagaccgc atggccgtgc gccgctgggg ggtggagccg aaccgctacg tgctctacgt
     3361 ctcgcgcctt gagccggaga acaacgcgcg gctcgtcatc gaagccttca agcgcgtgcg
     3421 cacttcgcac aaactcgtca tcgtcggcga tgcgccctac gcgcacgagt acatcgaaga
     3481 gttgcgcgcc agcgcgcggc gcgaccgtcg catcgtcttc accagtttcg tcttcggcga
     3541 tgcctaccgc gccctgcaac agaatgctta ttgctacgtc cacgccaccg aagtcggcgg
     3601 cacgcatccc gcgctgctcg aagcgatggg ctacggcaac tgcgtcctga cgcttgccac
     3661 gccagaaaac atagaagccg tcggcgaagc ggccatcccc tacagcgacg aaaaagacct
     3721 cgaagagaaa ctccaacgcg tcctgcgcga cggctcgctc gtcaactctt atcgccagcg
     3781 cgcgcagaag cgcgtgagcg aacattacga ctgggagcgc atcgttgacc tgtacgaaaa
     3841 tttgttcgcg cgcatggccg gacagcgcga accgcatcgc gccgtggccg aattggtcga
     3901 ggcggagtcg aacaaagaag ccgtgccgag gtagaacaat cgtgcaggcg tcatgccgcg
     3961 cgtgcgcggg gagacggagc cggagttaaa cagagaaagg gcgcgctgac gatccgttag
     4021 cgcgcccttt ggcctgttac tcgccccctc tgagtttgaa gggctacttc tccggggtcg
     4081 tcgctttgcc gtccagaccg cgcgcttcga ctttgccgag gttgagtttc aataactccg
     4141 gctcgatctt gctgcggtcg cccacggcga cgatgaccat tgattccggg agcaggtatt
     4201 tcccggccac gcgccgcacg tcggcgacgg tgacggcgtc aatcctgccc ggcagcgtgc
     4261 tgtaatagtc gagcggaaga ttgtagatga aaagctgtcc gatggtggcc gccgcctgcc
     4321 cggtcgtctc gaagagtccc ggcagcgagc gtgcgaaaga gtctttcgcg atcttcaact
     4381 cttccgcgct cacgtccgtc gtacgcatcc gctcgaactc gttgaaaatc tcgcgcaccg
     4441 ccggggctgt cacatccgtg cggacgctcg tggccgtgaa gaaggggccg ggaccacgcc
     4501 ggaactgaaa gaccgaaccc gcgccgtaag tgtagccgtt cttctcgcgc aggttcatgt
     4561 tgatgcggct ggagaagagg ccgccgagtc ccgtgttcat cacttcgatg ggcacgtagt
     4621 cggggttggc gcgcggtacg ccgaccatcc cgatgcgcaa ggccgtctgc ggcgcgcccg
     4681 gtttgtccac gagcaggatg cgacgcgctg cgccggtgcg cacttcggga cgcgtcaccg
     4741 tcccggcctt gcccgcccac ttgccgaaat gcttttcggc cagcgcgcgc agttcgctct
     4801 cgttgagatc gcccgcgacg acgagcgcgg cgttctccgg cacgtagccc gcctgccaga
     4861 acttcgccat ctcttcgcgc gtgatggctt tgattgactc ctccgtgccg agttccgtga
     4921 agccgtaagg gtggctcgtt ccgtagactt cgctgttgaa gactttgctg gcgagcacgt
     4981 tcgggttgtc gcgctgttgg aggacttggg tgaggcgcgt gttgcgcacg cggtcgagtt
     5041 ccttcgcggc gaaggcgggt tggagcgcaa cgtctgcgac gagttcgagc gcggcgtcgg
     5101 cgttcttctt caaggtgcgg acgctcaccg tcgaggcgtc gctcgaagaa cctgtgccga
     5161 gcgttgcgcc gatggccgcc acgtcttcgg cgatctgcaa ggtcgaacgc ttgttcgtgc
     5221 cttcgtcgag catgtccgcc gtgaacgaag cgaggccggg cttatccgcc gggttcgctt
     5281 cgctgccgct caggacgacg aggttggccg cgacgaccgg cagattgtgt tgctctacga
     5341 gcagcaccgt gaggccgttc gcgagtttga aggatttgg
//
LOCUS       DASWNS010000044         5324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_417343, whole genome shotgun sequence.
ACCESSION   DASWNS010000044 DASWNS010000000
VERSION     DASWNS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417343"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGB76_01565"
     CDS             <1..312
                     /locus_tag="VGB76_01565"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEY0097615.1"
                     /translation="GYSAKFRKRLKVGEMSERGVLWATRSGRAGFIARGIVFLIVGGF
                     LIQAAWYYDSSKAKGLDGALQNLIQQPFGPWLLGAVALGLIAYGLYMLVEARYRRIAG
                     S"
     gene            complement(324..1499)
                     /gene="aroC"
                     /locus_tag="VGB76_01570"
     CDS             complement(324..1499)
                     /gene="aroC"
                     /locus_tag="VGB76_01570"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY0097616.1"
                     /translation="MFRFTTAGESHGRALVAIVEGLPAGVRVEVESINREMERRQWGY
                     GRGGRMKIEQDRVEILSGVRHGLTLGSPVAMLVENKDWKNWTEVMSAEVREAESAPEK
                     SRRVKRPRPGHADLAGGLKYGTRDLRDILERASARETAARVACGALAKLLLANFELEI
                     LSHVVQLGGVPVQPLELKWDEIAAIADDAPLRCGDGEAQARMVELIDEKRREGDTLGG
                     VFEVVARGVVAGLGAHTSWDEKLDGRLAQALMSIPAVKAVAIGAGVEASALAGSEVHD
                     EIGYERESRRFTRPTNRAGGLEGGITNGEELRVRGHLKPISTLRRALRSVDIDTKEEE
                     AAAFERSDVTAVPAAGVIGEAMVALVLAQAMREKFGGDSLPEMRRNFDAYRQQLRDY"
     gene            complement(1753..3252)
                     /locus_tag="VGB76_01575"
     CDS             complement(1753..3252)
                     /locus_tag="VGB76_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0097617.1"
                     /translation="MLEKFKPDEVQSYLADASNLAGGHAARVVFPETTEEVAVMLAAA
                     SRERVPVTVSGAGTGVVGGRVPFGGIVLATDKLNRIGEIVPGANGGEASAEAGVALAD
                     FQRAVEARGLLYPPDPTERSCYLGATVATNSSGARTFKYGATRRYIRRLRVALATGDV
                     LNLSRGELFANEGRLRIPLGDGRNIEARLPAYRMPQTRKHAAGYYVAQGMDAIDLFIG
                     SEGTLGVVCEIEAALLSRPEGVLGGVVFFREEEKLLAFVRAARAASLDTRAGNLEGEL
                     DARALEFFDAEALNFLREKFPLIPRDAAGAIFFEQETKEHTEERLMDAWLALLERHEA
                     ALDASWFAANERDGARLREFRHALPLLVNEWLARHGQRKVSTDMSVPDEAFPAMLGFY
                     QETLRASRLSYVIFGHIGDNHVHVNILPRDDAQAATAREIYRRFIERAVRTGGTISAE
                     HGVGKIKRDYLRTLYGEEHLRQMAALKRAFDPACILGRGNMFAEEFLEQ"
     gene            complement(3255..3695)
                     /locus_tag="VGB76_01580"
     CDS             complement(3255..3695)
                     /locus_tag="VGB76_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097618.1"
                     /translation="MFEQTLSFPCPSCKEIINDRAEQCKYCGAPIDKGVAQFAAEVQG
                     KVNQAYSDASYLKSTAVALWTLLAISLLPFGACVFIGAVIIFFVLPVMLVRWHLRFNN
                     LNTTDPDYRKAKRNKNIALVLWLVVIPAFILRDLIFLVVRHMLR"
     gene            complement(3765..>5324)
                     /gene="uvrB"
                     /locus_tag="VGB76_01585"
     CDS             complement(3765..>5324)
                     /gene="uvrB"
                     /locus_tag="VGB76_01585"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY0097619.1"
                     /translation="YGLGDPDAYYNMLLFVEPGMKMARDALVQKLVELQYERSDIEFG
                     RGSFRVRGDIVEIFPSYQEQAYRIELWGDEIDTISTIDPLLGEVLHKHTTRLPIYPKT
                     HYVMSKPTIKRAIKTIREELDWWEPKLIQEGKLVEAQRLHQRTMYDLEMIKEMGFCRG
                     IENYSRHLTGKNPGDPPPTLLDYLPRDTMVVIDESHQTVPQVRGMFNGDQSRKRTLVE
                     YGFRLPSALDNRPLNFDEFNERIGQVIYVSATPGPYELTKSGGEVVEQIIRPTGLTDP
                     LVIVRPVKGQIDDLLEECRVRAERNERVLVTTLTKKMAEDLTEYFTEVGVKVRYLHSD
                     IETLERIKILRDLRRGEFDVLVGINLLREGLDLPEVSLVAILDADKEGFLRSESSLIQ
                     TIGRAARHSSGTAILYADRITDSMKRAIDETSRRRTIQEAYNEEHGITPQTIIKPIEA
                     TLVTAYEADYFKVPLELEAFEEYSRDKIDGTIARLEAEMRHAAKAMEFERAAELRDRI
                     KYLRERELTMT"
BASE COUNT          969 a         1712 c         1604 g         1039 t
ORIGIN      
        1 ggctactcgg cgaagtttcg caagcgtctg aaagtgggcg agatgagcga gcgcggagtg
       61 ctgtgggcga cgcgcagcgg gcgcgcgggc tttatcgcgc ggggcatcgt cttcctcatc
      121 gtcggcggct tcctcataca ggccgcgtgg tactacgact cctcgaaagc gaaaggctta
      181 gacggcgcgc tccaaaacct catccaacaa cccttcggac catggctgct cggagccgtc
      241 gccctcggcc tcatcgccta cggcctctac atgctcgtcg aagcacgcta ccggcgcatc
      301 gcaggctctt gaagattctg caatcagtag tcgcgcaact gttgacgata ggcgtcgaag
      361 ttgcggcgca tctccgggag actgtcgccg ccgaactttt cgcgcatcgc ttgtgcgagc
      421 acgagcgcga ccatcgcttc gccgatgacg cccgccgccg gaaccgccgt cacgtccgaa
      481 cgctcgaagg cggcggcctc ttcctccttc gtgtctatgt caaccgagcg tagcgcgcgc
      541 ctgagcgtcg agatcggctt gagatggccg cgcacgcgca actcctcgcc gttcgtgatg
      601 ccgccctcta agccgcccgc gcggttcgtc gggcgcgtga acctgcgcga ctcccgctca
      661 taaccgatct cgtcatgcac ctccgacccc gcgagcgcgc tcgcctccac acccgccccg
      721 atggcgaccg ccttcaccgc cggaatagac atcaacgcct gcgccagacg cccatccaac
      781 ttctcatccc aggacgtgtg cgcgcctaat ccggcgacca cgccgcgcgc caccacttca
      841 aagacgccgc cgagcgtgtc gccctcccgc cgcttctcgt caatcaactc gaccatcctc
      901 gcctgcgctt ccccatcgcc gcaacgcagc ggagcgtcgt ccgcgatagc agcgatctca
      961 tcccacttca attcgagcgg ttgcaccggc acgccgccga gttgcacgac gtggctcaat
     1021 atctccaact cgaaattagc gagcaataat ttcgcgagcg cgccgcaggc cacgcgcgcc
     1081 gccgtctcgc gcgccgacgc acgctccagt atgtctcgca ggtcgcgcgt gccgtacttc
     1141 aaaccgcccg cgaggtctgc gtgtccgggg cgcgggcgct tcacccgccg cgacttctcc
     1201 ggcgcgctct ctgcttcacg cacctctgcc gacatcacct ccgtccagtt cttccaatcc
     1261 ttgttctcga cgagcattgc gacgggcgaa ccgagcgtca gcccgtgacg caccccgctc
     1321 aaaatctcca cccggtcttg ctcgatcttc atccgcccgc cccgcccgta gccccactgc
     1381 cgccgctcca tctcacgatt gatcgactcg acttccacac gcacgcccgc aggcagacct
     1441 tcgactatcg ccaccagcgc gcgcccatga gactctccgg cagtagtgaa acgaaacata
     1501 agagaacagt gaccagtgac gggtgacaag tgacgagtga cgagcaagca ccggctaaaa
     1561 gtgtcgccat cgtttacgac tatcgtgtaa gtgacaagta acgagcaaca agtaatgagc
     1621 aacgagtaat gaacatgaag taacgaccgg gcagggacga tattttaact tgtcaatcgt
     1681 cgcccgtcac ggttctctta actcgtcacc cgtccctgtg ttcttaactc atcacttgtc
     1741 actcgtcact tgtcactgct ctaaaaactc ttccgcgaac atgttgccgc gaccgagaat
     1801 gcaggcgggg tcgaaggcgc gtttgagggc ggccatctgg cgcaagtgct cctcgccgta
     1861 gagcgtgcgc aggtagtcgc gcttgatctt gccgacgcca tgctccgccg agatcgtgcc
     1921 gccggtgcgg acggcgcgct cgataaaccg tctgtaaatc tcgcgcgctg tcgcagcctg
     1981 cgcgtcgtcg cgcggcagga tgttgacgtg gacgtgattg tcgccgatgt ggccgaagat
     2041 gacgtatgac aggcggctgg cgcgcagagt ctcctggtag aaaccgagca tcgccggaaa
     2101 cgcttcgtcg ggcacggaca tatccgtcga gactttgcgc tggccgtggc gcgcgagcca
     2161 ttcgttgacg agcagcggca gcgcgtggcg aaactcgcgc aggcgcgcgc cgtcgcgttc
     2221 gttcgcagcg aaccatgaag catcgagcgc ggcttcgtgt cgttcgagga gcgcgagcca
     2281 cgcgtccatc aagcgttctt ccgtgtgctc tttcgtctcc tgctcgaaga agatcgcgcc
     2341 cgcagcatcc ctggggatga gcgggaattt ttcgcgcagg aagttcaggg cttcggcgtc
     2401 gaagaactca agcgcgcgtg cgtccaactc gccttcgaga tttccggcgc gcgtgtcgag
     2461 cgacgccgcg cgcgctgcgc gaacaaaggc gagcaatttt tcttcttcac ggaagaagac
     2521 cacgccgccg agcacgccct ccggcctcga cagcaacgcg gcctcgattt cacagacgac
     2581 gccgagcgtg ccttcgctcc cgatgaagag atcaatcgcg tccatgccct gtgcgacgta
     2641 ataaccggcg gcatgttttc gcgtctgcgg catgcggtag gcgggcagac gcgcttcgat
     2701 gttgcgcccg tcgccgagcg ggatgcggag tcgcccttcg tttgcgaaga gttctccgcg
     2761 cgagaggttg agcacgtcgc ccgtcgcaag cgcgacccgc aggcggcgta tgtaacgacg
     2821 cgtcgcccca tacttgaagg tacgcgcgcc ggaactgttc gtcgccacgg tcgcgccgag
     2881 ataacaactg cgctcggtcg ggtcgggcgg gtagagcagc ccgcgcgctt cgacggcgcg
     2941 ttgaaaatcc gcgagcgcga cgcccgcctc tgcgctggcc tcccccccgt tcgcgcccgg
     3001 cacgatctcg ccgatgcgat tcaacttatc cgtcgccaac acgatgccgc cgaacggcac
     3061 gcgaccgccc acgacgcccg tacccgcgcc cgacaccgtg accggaacgc gctcgcgcga
     3121 agcggcggcc aacatcacgg cgacttcctc cgtcgtctcc ggaaacacga cgcgcgccgc
     3181 gtgcccgccc gcgagattgc tcgcgtcggc gagataggat tgcacctcgt cgggcttaaa
     3241 cttttccagc atccttatct caacatgtgt ctgacgacga gaaaaataag gtctcgcaga
     3301 atgaatgcgg gaatcaccac gagccacaga acgagagcga tgttcttatt gcgcttcgct
     3361 ttccggtaat cagggtcggt cgtgttcagg ttgttgaacc tcaggtgcca gcggacgagc
     3421 atcacgggca acacgaaaaa gataatgacc gcgccgataa agacgcaagc cccaaacggc
     3481 agcaggctga tcgccagcag agtccagagc gcaacagcgg tgctcttcaa gtagctcgcg
     3541 tcgctgtacg cctgattaac tttgccctgc acctccgcag caaactgcgc cacgccttta
     3601 tcaatcggtg cgccgcaata tttacattgt tccgccctgt cgttgatgat ctctttgcaa
     3661 ctcggacagg ggaaactcag tgtctgctcg aacatgtgtg cctccgaata attgtgagga
     3721 tgtaaccgga cgcgggcgat gaagagagac tccattaaac gcgatcacgt cattgtcagt
     3781 tcgcgctcgc gtagatactt gatgcggtcg cggagttcgg cggcgcgttc aaactccatc
     3841 gctttggcgg cgtggcgcat ctcggcttcg aggcgcgcga tggtgccgtc gatcttgtcg
     3901 cgcgagtact cttcgaaggc ttcgagttcg agcggcactt tgaagtagtc ggcctcgtag
     3961 gcggtgacga gcgtggcttc gatgggcttg atgatggttt gcggcgtgat gccgtgttcc
     4021 tcgttgtacg cctcttgaat cgtgcggcgg cgactcgtct cgtcaatggc gcgcttcatg
     4081 gaatcggtga tgcggtcggc gtagagaatg gccgtgcccg aagagtggcg cgcggcgcgc
     4141 ccgatggtct ggatgagcga tgactcggag cggaggaagc cctccttgtc cgcgtcgagg
     4201 atggcgacga gcgagacttc gggcaagtcc agaccttcgc gcaggaggtt gatgccgacg
     4261 agcacgtcga actcgccgcg ccgcaggtcg cgcagaatct tgatgcgttc gagcgtctcg
     4321 atgtccgagt ggagatagcg gactttcacg ccgacctccg tgaagtattc ggtcaaatcc
     4381 tccgccatct ttttcgtgag cgtggtgacg agcacgcgct cgttgcgctc ggcgcgcacg
     4441 cggcactctt ccagtagatc gtcgatctgt cctttgacgg ggcggacgat gactagcggg
     4501 tcggtcaagc cggtcgggcg gatgatctgc tcgaccactt cgccgccgga cttcgtcaac
     4561 tcataaggcc ccggcgtggc cgagacgtag atcacctgtc cgatgcgttc gttgaactcg
     4621 tcgaaattga gcgggcggtt gtcgagcgcg gaaggcaggc gaaagccgta ctcgacgagc
     4681 gtgcgcttgc gcgactggtc gccgttgaac atgccgcgca cctgcgggac ggtctggtgc
     4741 gattcgtcta tgacgaccat cgtgtcgcgc ggcaggtaat caaggagcgt cggcggtggg
     4801 tcgccgggat ttttcccggt caggtggcgc gaatagtttt cgatgccgcg acagaaaccc
     4861 atctccttga tcatctcaag gtcgtacatg gtgcgctggt ggaggcgttg cgcctcgacg
     4921 agtttgcctt cctgtatgag cttcggctcc caccagtcca actcttcgcg gatggtcttg
     4981 atggcgcgct tgatggtcgg cttcgacatc acgtagtgcg tcttcggata gatgggcagg
     5041 cgcgtcgtgt gcttgtgcag gacttcgccg agcaaagggt cgatggtcga gatggtgtcg
     5101 atctcgtcgc cccagagttc gatgcggtag gcttgctcct gataactcgg gaagatctcc
     5161 acgatgtcgc cgcgcacgcg gaaactcccg cgcccgaact ctatatcgga acgttcgtat
     5221 tgcagctcga ccagtttttg cacgagcgcg tcgcgcgcca tcttcatgcc cggctcgacg
     5281 aagagcagca tgttgtaata agcgtcaggg tcgccgaggc cgta
//
LOCUS       DASWNS010000045         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_27309, whole genome shotgun sequence.
ACCESSION   DASWNS010000045 DASWNS010000000
VERSION     DASWNS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27309"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1409)
                     /gene="rlmB"
                     /locus_tag="VGB76_01590"
     CDS             complement(318..1409)
                     /gene="rlmB"
                     /locus_tag="VGB76_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY0097620.1"
                     /translation="MKNFRKHKSKPLSTRARDDGRERREPRVEEKRDGDAPRRPEANR
                     NGVRRDEVRAGEHRNEVRRNEVGTHERRHEVRTSGANETREAPAKRVESAPDESHLYG
                     VSPVLEALRAGHRRIERITIAEGAGGARFAQLFELAREFRVPVRRAPRAELQRMAAVG
                     ANHQGVVAHVAAVAYAEADELLDALTARVGTGDPPLAVVLDGVEDPRNLGAIIRTAEC
                     AGVHGLFVPERRAVGLTETVAKASAGALEYLPVARATNIARLVEEFKERGIWTVGTDA
                     EANIAYTDWDWTQPCALLFGGEGAGLRRLVRERCDALVSIPLHGRISSLNVSVAAAVV
                     LYEAVRQRTLARPATYEEGRDGDEATEAG"
     gene            complement(1406..2455)
                     /locus_tag="VGB76_01595"
     CDS             complement(1406..2455)
                     /locus_tag="VGB76_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097621.1"
                     /translation="MAKEDDELPLMDDDAGAPLMARAFAPGEMVACEACVRANPPTRM
                     NCLYCGAVLPVGDAENDLRRPVLKALEAWEQGFNVVLVPQGAADELSVDKLSEAAAFL
                     RVETGQLREMLRAGVYLPLARVAARDEASLVERRVRALGCRVETVADEELGVETHAPK
                     RIRRLEIGADALTGWSSAESEAERVEWPEVALIVAGRIARKRIEVEERRGGRRAEAEV
                     VETREFQTDESALDLYFKGSVVNWRIMAENFDYTCLGAEKCLTAAENFKRLAETLRVR
                     ATAADFDDSYRSVRALLKFAWPLAEQTEAGSLKRVRPGRYNAEAVTSVTNETQFTRYG
                     RLLHYFRLRSLSTLT"
     gene            2757..>3053
                     /locus_tag="VGB76_01600"
     CDS             2757..>3053
                     /locus_tag="VGB76_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097622.1"
                     /translation="MLQTCLGKLCHARRVNFRFALVVSLVSLACASSSCAGARDPDTG
                     RPRANEPPYPVVLAANAERREQALAGWAALARDNNLQNEPPPQLQPVTATISALP"
BASE COUNT          525 a         1114 c          853 g          561 t
ORIGIN      
        1 ccctcatcca tggtcgccgg tttgcgcttc ttctctttgc ggcgcgcgac aattttatcc
       61 tcgtcgagtt tgcccaacag ggcttgcggc accaggcgta tgtctgcgcc gcacgcacgg
      121 caaaacttcg cgtcgtctaa attttgcacc gcacatcgcg gacagtacat gcgcgtcctc
      181 ctcctccggg gaaagtcagg tgacagggca aggggaaaag ggagcggacg aagcgaaacg
      241 cacctttcga cgggcggaac aggtcaatct ctttcttaac ctttacccgc tagccctctc
      301 cctttcaact gacttctcta ccctgcttcc gttgcctcgt cgccgtctcg cccctcttca
      361 tacgtcgcgg gacgcgcgag ggttcgttgt cgcaccgctt cgtagagcac aacggcggcg
      421 gcgaccgaga cgttgagcga cgaaatgcgc ccgtgcaagg ggatactgac gagcgcatcg
      481 cagcgttcgc gcacgaggcg acgcaagccc gcgccctcgc cgccgaagag gagcgcgcac
      541 ggttgcgtcc aatcccaatc ggtgtaggcg atgttggcct cggcgtcagt cccgaccgtc
      601 cagatgccgc gctccttgaa ctcttcgacg agccgcgcga tgttcgtcgc acgcgcgacg
      661 ggcaggtatt cgagcgcgcc cgcgctggcc ttcgccaccg tctccgtcaa accgacggcg
      721 cggcgttcgg gcacgaacag gccgtggacg cccgcgcatt cagccgtgcg aatgatcgcg
      781 cccagattgc gcgggtcttc cacgccgtcg agcacaacgg cgagcggcgg gtcgcctgtg
      841 ccgacgcgcg ccgtcagcgc gtccaacagt tcatccgctt ccgcgtaagc gacggcggcg
      901 acatgcgcga ccacgccttg atgattcgcg cccaccgccg ccatccgctg caactccgcg
      961 cgcggcgcac gcctgacggg cacacgaaac tcacgcgcca gttcaaacaa ctgcgcgaag
     1021 cgcgccccgc ccgcgccctc cgcaatcgtg atccgctcga tgcgccggtg acccgcgcgc
     1081 aaggcttcga gcacgggcga cacgccgtag agatgcgact cgtcgggcgc gctctccaca
     1141 cgttttgccg gagcctcccg cgtctcattc gcgccgctcg ttctaacttc atggcgtcgc
     1201 tcgtgcgtgc cgacttcgtt gcgccgcacc tcattccggt gctcgcccgc gcggacttca
     1261 tcgcggcgca cgccgttccg gttcgcttcg gggcggcgcg gcgcgtctcc gtcgcgcttc
     1321 tcttcgacgc gcggttcgcg tcgctcgcgc ccgtcgtcgc gtgcgcgggt cgagagcggc
     1381 ttcgatttat gcttgcgaaa atttttcatg tgagcgtcga gagtgagcgc aggcgaaagt
     1441 agtgcaagag tcgcccgtag cgcgtgaact gcgtctcatt cgtcacgctc gtcacggctt
     1501 ctgcattata gcggcctggg cgtacgcgct tgaggcttcc cgcctccgtc tgctccgcga
     1561 gcggccacgc gaacttcaat aaggcgcgca cgctccggta agaatcatcg aagtcggcgg
     1621 cggtcgcgcg cacgcgcagc gtctccgcga gtcgtttaaa attctccgcc gccgtcagac
     1681 acttctccgc gccgagacac gtataatcaa aattctccgc catgatgcgc cagttgacga
     1741 cagacccctt gaagtagaga tcgagcgcac tctcatccgt ctgaaactcg cgcgtctcga
     1801 ccacttccgc ctccgcgcga cgcccgccgc gccgctcctc cacttcgatc cgcttccgtg
     1861 cgatgcgccc cgccacgatc aacgccacct ccggccattc gacgcgctcc gcctcgctct
     1921 ccgcgctgct ccaacccgtg agcgcgtccg cgccgatctc aagcctgcga atccgcttcg
     1981 gcgcgtgcgt ctcgacgccc aactcttcat ccgccaccgt ctcgacccgg cagccgagcg
     2041 cgcgaactct tctctccacc agcgacgcct catcgcgcgc agccacgcgc gccagaggca
     2101 aatacacgcc cgccctcaac atctcgcgca actgccccgt ctccacacgc aggaacgcag
     2161 ccgcctccga caacttatcc acactcaact catccgccgc cccctgcggc acgagcacga
     2221 cgttgaaccc ctgctcccac gcctccagag ccttcaacac cggacgacgc agatcattct
     2281 cggcatcgcc caccggcaac accgccccgc aatacagaca attcatacgc gtcggcggat
     2341 tagcacgcac gcacgcctcg cacgccacca tctcccccgg cgcaaacgcc cgcgccatca
     2401 acggcgcgcc cgcgtcatca tccatcaacg gcagttcatc atcttccttc gccaagtctt
     2461 ttgctcctgt ccctcgatta tacgtcccgc cccccgcttt gcaatcacca ttcaaaggca
     2521 aacttcaaac cagccacgta gagcgcagga aatggaagca acacgaggag gaggaactta
     2581 agggcttccc gcgaaagtgt aggctgtggt cggttgcgcc atagaatctt gcgcgggata
     2641 aaatatccgc cctgcctcat catcccaccc gcgatgcagt ataatcgcgg cgcgtcttga
     2701 tttatcaact gctttgagag cgtcattcac cagcgaattt agagcgtcgg atttttatgc
     2761 tgcaaacctg cctggggaaa ctttgtcatg cgcgtcgggt caactttcgt ttcgcgctcg
     2821 tcgtgtcgct cgtctctctg gcgtgcgcgt cgtcatcgtg cgcgggtgcg cgcgacccgg
     2881 acaccgggcg accgcgtgcg aacgagccgc cctatccggt tgtgctcgcc gcgaatgcgg
     2941 aacggcgcga gcaggcgttg gcgggttggg ctgcgctggc gcgcgacaat aatttgcaga
     3001 acgaaccgcc gccgcagttg cagcccgtca cggcgacgat cagcgcgctg cca
//
LOCUS       DASWNS010000046         6236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_469532, whole genome shotgun sequence.
ACCESSION   DASWNS010000046 DASWNS010000000
VERSION     DASWNS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469532"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VGB76_01605"
     CDS             <1..574
                     /locus_tag="VGB76_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097623.1"
                     /translation="HSGLGTAAERAWTDRSKAPGNEVVRLRRVRVHAADGQTAEAIDI
                     RQPVGLEMEFEVLQAGHVLVPNFGLYNEEGVLLFIAVDSDPAWRGKERTVGRHTSTAW
                     IPGNFFAEGSLTVNAALSTFDPVYVHASETEAVAFQVVDSMDGDSARGGFAGVMPGVI
                     RPLLRWENKFSGDEAFAPEMSPATTTAHLS"
     gene            627..1877
                     /locus_tag="VGB76_01610"
     CDS             627..1877
                     /locus_tag="VGB76_01610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar O-methyltransferase"
                     /protein_id="HEY0097624.1"
                     /translation="MFSNVISRIGYKIGLTPFNVHLLRGLKRPEWLYRNRKSIPFQPL
                     YFAPVAAVTPEDVALCERLIAAYRKATGVERTGAVSEIWTTMLTRYGKLQKALDEGRP
                     ENLAQTLSTMFREAFLFGISTADTYPGSQTRIGARIWSQRCLEDLVSLAEYVGAVRTE
                     CLEQGELGWAFKDGAEALVAKIERAIGTPVNFPAIGAPYGIRIGDALITFETPEHIYV
                     ATRINEAINAYVEESRRGAPHIVEIGAGFGGTAHWLLKLRRDVASYTIIDLPLINVLQ
                     GYFLAKAFGAANVSLYGEEGDARRDATVAAGAATGTFINVRPDHSLNSVGGAGVDVLV
                     NENSMPEMPQHVVENYLAWAKRRVSGIFYSYNHEAYSPFDNVPQVLVPEVLAGVGGFK
                     RLSRNYSWVRRGYVEEVYARDHQD"
     gene            1922..2638
                     /locus_tag="VGB76_01615"
     CDS             1922..2638
                     /locus_tag="VGB76_01615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0097625.1"
                     /translation="MLNIRGQLHNTKKWLTRRTQLGKLDTIEAIKARHADEFVRLRDM
                     IRASGTDSLSHFGSSYTHEGGLCLQQNPDEFASLILILKERQPYASYMEIGSASGGAC
                     LILQREVGFERVMSLDNGEHPRAVEQKKNFSQIPNFRQFLGDSHSAEARAYLQQNVEG
                     KLDVAFIDGDHSYEGVWQDIELTLPFCRPGTLIILHDTVACDGVERAWLRCVREGRIK
                     PVAEYIGEVRPLGIGVGTVI"
     gene            2656..3426
                     /gene="rfbF"
                     /locus_tag="VGB76_01620"
     CDS             2656..3426
                     /gene="rfbF"
                     /locus_tag="VGB76_01620"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HEY0097626.1"
                     /translation="MKVVILAGGLGTRLAEETEVKPKPMVEIGGRPILWHIVKHYAHY
                     GFKEFFIALGYKGELIKRYFVDYCNLNGSMTLDMASGKIDAHDKDCEDWILHLKNTGQ
                     ATSTGGRVKRLEPWLKDATFMLTYGDGVCDIDLQELLRFHRAHGKLATVTAVRPPARF
                     GGLVFDGDLVSDFTEKPQIGEGWINGGFMVLEPGIFDYLSGDESVLEADALEQLAADR
                     QLAAYRHEQFWQCMDTLRDKRLLENLWHEGRAPWKVWE"
     gene            3490..4500
                     /locus_tag="VGB76_01625"
     CDS             3490..4500
                     /locus_tag="VGB76_01625"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0097627.1"
                     /translation="MVERSMTGNGSGGFWLDRPTLVTGATGLIGGWLVRRLLEAGADV
                     VCLVRDTVPQSEFVRAGLDARVKTVRGAVEDQELMERTLGEYEVDTVIHLAAQTIVGI
                     ANRNPVSTFEANIAGTWSLLEAARRSPTVKGIVVASSDKAYGDHEILPYSEDAPLQGR
                     HPYDVSKSCADLIAQMYGYTYKLPVAITRCGNFYGGGDLNWNRIVPGTIRSVLRGQRP
                     VIRSDGQFIRDYFYAEDGALANMTLAEKLAANPDLRGHAFNFSNEIQVTVLELVRRIL
                     ALMNSTLEPEVRNEATNEIRHQYLSAAKAREMLDWQPRYTLDEGLGRTIEWYKKFLEV
                     AE"
     gene            4497..5741
                     /locus_tag="VGB76_01630"
     CDS             4497..5741
                     /locus_tag="VGB76_01630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0097628.1"
                     /translation="MNAKEALPPCRACAAPALELILSLGHTPLANALLTAEQLREPEA
                     TYPLDLAFCPACTLVQITETVPPEQLFREYFYLSSFSDTMLRHASELTARLIGERGLG
                     AASLAVEVASNDGYLLQYYKGAGVPVLGIEPAANIARIATEERGIPTVCEFFGEELAA
                     KLAGQGERADVIHANNVLAHVADLNGFVRGLELLLKDDGVAVIEVPYVKEMIDHREFD
                     TIYHEHLCYFSLTALERLFRRHHLRIEDVELLPLHGGSLLIIAGKERGAGVVSERVTR
                     MLAEEAAWGVGRLEFYRGFGQGVARLRDELLALLNSLKAEGKRIAVYGASAKGSTLLN
                     YFRLGRETLDFVVDRSTVKQGYYTPGTHLPIHAPEMLLTEMPDYVLLLTWNFADEILA
                     QQSAYRVRGGRFILPIPEVKVV"
     gene            5826..>6236
                     /locus_tag="VGB76_01635"
     CDS             5826..>6236
                     /locus_tag="VGB76_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEY0097629.1"
                     /translation="MIFNETKLAGAFIIEQELFEDQRGFFARSWSEREFAAQGLDARL
                     AECNISFNHREGTLRGMHFQNAPHAQAKLVRCTQGAIYDVIIDLRPASPTFKGWIGVE
                     LTAENRRMLFVPEGFAHGFQTLAPNTEVFYQMSEV"
BASE COUNT         1231 a         1885 c         2007 g         1113 t
ORIGIN      
        1 gcattcgggt ttgggcacgg cggcggaacg cgcctggact gatcgcagca aagcgccggg
       61 caacgaagtc gtgcgcttgc gacgcgtgcg cgtccacgcc gccgacgggc agacggcgga
      121 ggcgatagac attcggcagc cggtcgggct ggagatggaa ttcgaagtct tgcaggcagg
      181 ccacgtgctc gtgccgaact tcggcctgta caacgaagaa ggcgtgctgc tcttcatcgc
      241 cgtcgattcc gacccggcgt ggcgcggtaa agagcgcacg gtcgggcggc atacgagcac
      301 cgcttggatt cccggaaatt ttttcgccga gggcagcctg acggtcaatg cggcgttgag
      361 cacgttcgat cccgtctacg tccacgccag cgagacggag gccgtggcct ttcaggtcgt
      421 ggacagcatg gacggcgatt cggcgcgcgg cgggttcgcg ggcgtgatgc ccggcgtgat
      481 caggccgctg ctgcgttggg agaataagtt cagcggggac gaagcgttcg cgccggagat
      541 gtctccggcg acgacgacgg cgcatctctc gtaaacgcac gcgtgcgacg accgggcggc
      601 gcgagtttat aagtttacaa ggacacatgt tttcaaacgt catatcgcgc atcgggtata
      661 agatcggctt gacgcctttt aacgtgcatt tgctgcgcgg cttgaagcgg cctgaatggc
      721 tctatcgcaa tcgcaagagc atcccgtttc agccgctcta ctttgcgccc gtcgccgccg
      781 tcacgccgga agatgtcgcc ctctgtgaac gcctgatcgc ggcctaccgg aaggcgaccg
      841 gcgtggagcg cacgggcgcg gtcagcgaaa tctggacgac gatgttgacg cgttacggga
      901 agttgcagaa ggctctggac gaaggacgcc ccgaaaatct ggcgcagact ttatccacca
      961 tgttcagaga ggcgtttctg ttcggcatca gcacggctga tacctacccc ggcagccaga
     1021 cgcgtatcgg agcgcgcatc tggtcgcagc ggtgtctgga agacctcgtc tcgctggccg
     1081 agtacgtggg cgcggtcaga acggaatgtc tcgaacaggg cgaactcggt tgggctttca
     1141 aggacggagc ggaagcgttg gtcgcaaaga tcgagcgcgc catcggcacg cccgtcaact
     1201 tcccggctat cggtgcgcct tacggcatca ggatcggcga tgctctgatc accttcgaga
     1261 cgcccgagca catttatgtc gcgacgagga ttaacgaagc gatcaacgct tacgtggagg
     1321 agtcgcgccg gggcgcgccg cacatcgtcg agatcggcgc gggcttcggc ggcacggcgc
     1381 actggctgtt gaaactcagg cgcgacgtgg cgagctacac gatcatcgat ctgccgctca
     1441 tcaacgtctt gcagggatat tttttagcca aagcgttcgg cgcggcaaac gtctctctct
     1501 acggagaaga gggcgacgct cgcagggatg cgaccgtcgc agcaggcgcg gccacgggaa
     1561 cgttcatcaa cgtgcgaccg gatcattcgc tgaactctgt gggcggcgcg ggagttgacg
     1621 tgctcgtcaa cgaaaacagt atgccggaga tgccgcaaca cgtggtggag aattatctgg
     1681 cgtgggcgaa gcggcgggtc agcggcatct tctacagtta taatcacgaa gcgtattcgc
     1741 ctttcgataa tgtgccgcag gtgttggtgc cggaagtgct ggcgggcgtg ggcggtttca
     1801 aacgcttgtc cagaaattat tcgtgggtgc ggcgcgggta tgtggaggaa gtttacgcgc
     1861 gcgaccatca ggattgatca agccgcgttc gggtcttaac gggcgaggga gaggaagaga
     1921 gatgcttaat attcggggtc aactacacaa cactaaaaaa tggctcacgc gccggacgca
     1981 actcggcaaa ctcgatacca tcgaagctat caaagcgcgc cacgccgacg agttcgtgcg
     2041 cctgcgcgac atgatccgcg cctccggcac ggacagcctc tcgcacttcg gcagcagcta
     2101 cacgcacgaa ggcggcctct gcctgcaaca gaacccggac gagtttgcct cgctcattct
     2161 gatcctcaaa gagcggcagc cctacgcgag ttatatggag atcggttcgg cgtcgggcgg
     2221 cgcgtgcctc attctccagc gcgaggtcgg gttcgagcgc gtgatgagtc tcgacaacgg
     2281 cgagcacccg cgcgccgtcg agcagaagaa aaatttcagc cagattccca acttccggca
     2341 gtttctcggc gatagccatt cggcggaggc gcgcgcttat ttgcagcaga acgtggaagg
     2401 caaactggac gtggccttca tcgacggcga ccacagctac gagggcgtct ggcaggacat
     2461 cgaactgacg ctgcccttct gtcgtccggg cacgctcatc atcctgcacg acacggtggc
     2521 ctgcgacggc gtggagcgcg cgtggctcag gtgcgtgcgc gaggggcgca tcaaacccgt
     2581 cgcggagtat atcggcgaag tcaggccgct cggcatcggc gtcgggacgg tgatctgaag
     2641 gacggggcgt aagcgatgaa agttgtgata ctggcagggg gcttgggcac gcgcctcgcc
     2701 gaagagacgg aggtgaagcc gaagccgatg gtcgagattg gcgggcggcc tattctctgg
     2761 cacatcgtca aacactacgc gcactacggc ttcaaagagt tcttcatcgc gctcggctac
     2821 aagggcgagt tgatcaagcg ttacttcgta gactactgca acctcaacgg cagcatgacg
     2881 ctcgacatgg cgagcggaaa gatagacgcg cacgacaaag attgcgaaga ctggattctg
     2941 cacctcaaga acaccgggca ggcgacgagc acgggcgggc gcgtcaagcg tctcgaaccg
     3001 tggctcaaag acgccacctt catgctcact tacggcgacg gcgtgtgcga catcgacttg
     3061 caggagttgc tgcgcttcca tcgcgcgcac ggcaaactgg cgaccgttac cgccgtgcgc
     3121 ccgcccgcgc gcttcggcgg cctcgtcttc gacggcgatc tcgtctccga cttcacggag
     3181 aagccgcaga tcggagaagg ctggatcaac ggcggcttca tggtgctcga accgggcatc
     3241 ttcgattatc tgagcggcga cgagagcgtg ctcgaagccg acgcgctcga acaacttgcc
     3301 gccgaccgtc aactcgccgc ttatcgccac gagcagttct ggcaatgcat ggacacgctg
     3361 cgcgataaac gcctgttgga aaacctctgg cacgaagggc gcgcgccgtg gaaagtctgg
     3421 gagtagacgc gagcggaaac ctatgcggga aaagttaatg agagagagcg agcggatgtc
     3481 gaaaagcgaa tggtagaacg tagcatgaca ggtaatggca gcggcggctt ctggctggat
     3541 cgcccgacgc tggtgacggg cgcgaccggc ctcatcggcg gctggctcgt gcgtcgtctg
     3601 ctggaagcgg gcgcagacgt ggtgtgcctc gtgcgcgaca cggttccgca gagcgagttt
     3661 gtgcgcgccg gactcgacgc gcgcgtcaag actgtgcgcg gcgcggtcga agatcaggag
     3721 ttgatggagc gcacgctcgg cgagtatgaa gttgacacgg tcatccacct cgcggcgcaa
     3781 accattgtcg gcatcgccaa ccgcaatccg gtttcgacct ttgaagccaa catcgccggg
     3841 acgtggtcgc tgctggaagc ggcgcgcagg agtcccacgg tgaaggggat cgtcgtcgcc
     3901 tcgtccgaca aagcctacgg cgaccacgaa attctgccct acagcgaaga cgcgcctttg
     3961 caggggcgac acccttacga cgtgagcaag tcgtgcgccg acctgatcgc gcagatgtac
     4021 ggctacacct acaaactccc cgtggcgatc acgcgctgcg gcaacttcta cggcggcggc
     4081 gacctcaact ggaatcgcat cgtgcccggc acgatccgct ccgtcctgcg cgggcaacgt
     4141 cccgtcatac gctccgacgg ccagttcata cgcgactact tctacgccga agacggcgcg
     4201 ctggcgaaca tgacgctcgc cgaaaagttg gccgccaatc cagacctgcg cggccacgcc
     4261 ttcaacttct cgaacgagat tcaggtgacc gtgctcgaac tcgtcaggcg catcctcgcc
     4321 ctgatgaatt ccacgctcga acccgaagtg cgcaacgaag cgacgaacga aattcgccat
     4381 caatatttga gcgcggcgaa agcgcgcgag atgctcgact ggcaaccgcg ctacacgctc
     4441 gacgaaggac tcgggcgcac gattgagtgg tacaaaaaat ttctggaggt ggcagagtga
     4501 acgcgaaaga agccctgccg ccctgtcgcg cctgcgccgc gcccgcgctc gaactgatcc
     4561 tctcgctcgg ccacacgccg cttgcgaacg ccctgctcac cgccgaacaa ctgcgcgaac
     4621 cggaagcgac ctacccgctc gatctagcct tctgccccgc ctgcacgctc gtgcagatca
     4681 cggagacggt gccgcccgaa cagttgttcc gcgaatactt ttatctctcg tccttctccg
     4741 acacgatgct caggcacgcc tccgagttga ccgcgcggct catcggcgag cgcgggcttg
     4801 gggcggcgag tctcgccgtg gaagtggcga gcaacgacgg ttatctcttg cagtattaca
     4861 agggcgcggg cgtgcccgtt ctcggcatcg aaccggcggc caacatcgcg cgcatcgcca
     4921 cggaagagcg cggcatcccc accgtctgcg agtttttcgg cgaagagttg gccgcgaaac
     4981 tggcgggaca gggagagcgc gccgatgtca ttcacgccaa caacgtgctg gcgcacgtcg
     5041 ccgatttgaa cgggttcgtg cgcgggttgg agttgttgtt gaaggacgac ggcgtcgccg
     5101 tcatcgaagt gccttacgtg aaggagatga tcgaccaccg cgaattcgac accatctatc
     5161 acgagcatct ctgctacttt tcgctgaccg ccctcgaacg cctcttccgc cgccaccacc
     5221 tgcgcatcga ggacgttgaa ctgctgccgc tgcacggcgg ctcgctcctc attatcgcgg
     5281 gcaaggagcg cggcgcgggc gtcgtctcgg agcgcgtcac gcggatgctg gcggaagagg
     5341 ccgcgtgggg cgtcgggcgt ttggagttct atcgcggctt cgggcagggc gtggcgcgcc
     5401 tgcgcgatga actgctcgcg ctgctgaact cgttgaaggc ggagggcaaa cgcatcgccg
     5461 tctatggcgc gtcggcgaag ggcagcaccc tgctcaacta ctttcgtctg gggcgcgaaa
     5521 cgctcgactt cgtcgttgac cgcagcaccg tcaagcaggg ctactacacg ccggggacgc
     5581 acctgccgat tcacgcgccg gagatgctgc tcacagagat gccggattac gtcttgctgc
     5641 tcacctggaa cttcgcagac gaaattctcg cgcaacagtc cgcctatcgc gtgcgcggcg
     5701 ggcgattcat tctccccatt ccggaagtga aagtggttta actaaggagc gcgcgaggct
     5761 gacgacgcgg taagagttta ccgcccgtcg tctcttctgc gcgcgctccc ggctgtcgtc
     5821 tgtttatgat cttcaacgaa acaaaactcg cgggcgcgtt catcatcgag caggaattat
     5881 tcgaagacca gcgcgggttc ttcgcgcgca gttggtcgga gcgcgagttc gcggcgcaag
     5941 ggttggacgc gcgcctcgcc gagtgcaaca tctctttcaa ccaccgcgag ggcacgctgc
     6001 gcgggatgca cttccagaac gcgccccacg cgcaggccaa actcgtccgc tgcacacagg
     6061 gcgcgatcta cgacgtgatc atagacctgc gccccgcctc gccaaccttc aaggggtgga
     6121 tcggggttga actgacggcg gagaatcgcc gcatgctctt cgtgccggaa ggattcgcac
     6181 acggtttcca gacgctcgca cccaacacgg aagtcttcta ccagatgtcg gaggtg
//
LOCUS       DASWNS010000047         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_338907, whole genome shotgun sequence.
ACCESSION   DASWNS010000047 DASWNS010000000
VERSION     DASWNS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338907"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1217)
                     /locus_tag="VGB76_01640"
     CDS             complement(213..1217)
                     /locus_tag="VGB76_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0097630.1"
                     /translation="METETERSRREGSATETPAACPSCNAALVDGMRFCRMCGYRLGE
                     GLAEYVQTERFDRMPGISAMPQGNQDLTGRTGGQTTVMTPPLAPVAPVWKPKRGRKWT
                     WMLLVFAVFLVIIMGGGGVFRAVRNAAQQGISRLQPPPEPPNSFLGVSEFSDTEGESG
                     ALLEETLPETPAARAGLLDGDIITKFDGRDVKDEDSLRDVISETPIGKTVEVVYLRDG
                     ETQTTKLTTISNKEYDLDAYMPKERGMMGVDDLARVPVEGTKIHGVLVGDVYDNRPAD
                     LAGLKEGDIITEFDGKPVRTAEGLGGYIAFSKPASTVKLIIYREGQRIELPVKMGRRN
                     "
     gene            1430..1651
                     /locus_tag="VGB76_01645"
     CDS             1430..1651
                     /locus_tag="VGB76_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097631.1"
                     /translation="MEVVKALAALQPLYGKDNIEIVTEDGARVRGVVKSMKTTQALTK
                     PSVKIQRADGEIVKITFDTITEIVDHNSA"
     gene            1838..2464
                     /locus_tag="VGB76_01650"
     CDS             1838..2464
                     /locus_tag="VGB76_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097632.1"
                     /translation="MSNITIQEFGGRVASVWEGLRPSTRSLMERALVSAAPVSMRGAS
                     APYDARSEWELSRLLAALDERTKEAGARVLSREEERELTRMAETCAHVLHSEARSAEV
                     FAQLLERALSVHDYARIDVLADTFSARLASSEMCELARHPNPSVRALAHEALAQSPTG
                     VLVELLGDPVDSDVARNALELQADDYGSEEARWVVSALERADASEEEA"
BASE COUNT          503 a          782 c          741 g          486 t
ORIGIN      
        1 tgaactgttc gaacccgacg aggccgaaga agatcgcgaa gatgtccgcc gcgaaatgga
       61 agccatgatg agagacaaga tgaacccgga aggatgagac aggaatcaag tgtcagaaga
      121 cagaatacag gagtcagaat aaatacaagc gcggcgcgtt tattctgact cctgtattct
      181 gtcttctgac ttctgttttc tgactccgcc ctttaattgc gccgtcccat cttgacgggc
      241 aactcgatgc gttggccttc gcggtagatg atgagtttga cggtgctggc gggcttggag
      301 aaggcgatgt agccgccgag tccttcggcg gtgcgcacgg gtttgccgtc aaactccgtg
      361 atgatgtcgc cctctttgag tccggcgagg tctgcggggc gattgtcgta aacatcgccg
      421 acgagcacgc cgtgaatctt cgttccctcc accggcacgc gagccagatc atccacgccc
      481 atcatgcctc tctctttcgg catgtacgcg tcgaggtcgt actccttatt cgagatggtt
      541 gtgagtttgg tggtctgcgt ctcgccgtcg cgcaggtaga cgacctcgac cgtcttgccg
      601 atgggcgttt cgctaatcac gtctctcaga gagtcttcgt ctttaacgtc ccttccgtca
      661 aacttcgtga tgatgtcgcc gtcgagaagt ccggcgcggg cggcgggagt ttcaggcaga
      721 gtctcttcga gcagcgcgcc actctcgcct tcggtatcgc taaactccga cacgccgagg
      781 aacgaattcg gcggctccgg cggcggctgt agacggctga tgccctgctg cgccgcattc
      841 ctgactgccc tgaacacccc ccccccgccc attattatca ccagaaagac ggcgaacacc
      901 agaagcatcc acgtccattt tctgccgcgc ttcggcttcc acacgggcgc gacgggcgcg
      961 agcgggggcg tcatcacagt cgtctgcccg cccgtcctac ccgtcaaatc ctggttaccc
     1021 tgcggcatgg cggatatgcc gggcatgcgg tcgaaacgct ccgtctgcac gtattcggcc
     1081 aaaccttcgc cgagacgata gccgcacatg cggcaaaaac gcatcccgtc cacgagcgcg
     1141 gcattgcaac tcgggcacgc ggcgggcgtc tccgtcgcgc tgccctctct gcgggagcgt
     1201 tctgtttccg tttccataaa aatcacctgt cgtcgggctt gcaaatggac gctcgcgggc
     1261 gtctgatcca ttttttcgcg tcacgaatta tacgtcaact ggcgcaaccc ggttgcacat
     1321 tcatacgcgc gcggcataaa cttttccgcg tatacaaacg acgaccggcg accgagacgc
     1381 ggcagacgaa agcgccggaa actgtctcaa gaaagaatga ggacccgacg tggaagtagt
     1441 taaagccctc gctgctttgc agccgctcta cggcaaagac aatatcgaga tcgtgaccga
     1501 ggacggcgca cgcgtgcgcg gcgtcgtcaa gagcatgaag acgacgcagg cgctcacgaa
     1561 acccagcgtt aaaatccaac gagccgacgg cgagatcgtc aagatcactt tcgacaccat
     1621 cacggagatc gtggatcaca actcggccta gcgtgcgcgg tctgtgcgcg gtaaacgtcg
     1681 aagattattt ttccttcgtg cgtggacgcg tcgctcgcgg cagtcggctt cgctttcgga
     1741 acccgtctct tcgtcgcggg tagtttataa ggtatgatat ttcacgcgcg ggcggccaac
     1801 ggaagagacg cccggcgcgc agggaattat gcagaaaatg tccaacatca cgattcagga
     1861 gttcggcggg cgcgtggcga gcgtgtggga gggtttgcgc ccttcgacgc gcagcctcat
     1921 ggaacgcgct ctcgtctccg ccgctcccgt ctccatgcgc ggcgcgagcg cgccttacga
     1981 tgcgcgctcg gaatgggaac tgagccgcct gctcgccgcg ctcgacgaac gcacgaaaga
     2041 ggctggcgca cgcgtcctga gccgcgaaga ggagcgagaa ctcacgcgca tggccgaaac
     2101 ctgcgcgcac gtcctgcaca gtgaagcgcg ttcggcggaa gtcttcgcgc aactgctgga
     2161 gcgcgccctg agcgtgcacg actacgcacg cattgacgtg ctcgcagaca ctttcagcgc
     2221 gcgtctcgcg tcgagcgaaa tgtgcgaact cgcgcgccat ccgaacccga gcgtgcgcgc
     2281 cctcgcccac gaagctctcg cgcaatcgcc gaccggcgtg ctcgtcgaac tgctcggcga
     2341 cccggttgac tcagacgtgg cgcgcaacgc cctcgaactt caggccgacg attacggctc
     2401 ggaagaagcc cgctgggtcg tcagcgcact cgaacgcgcc gacgccagcg aagaggaagc
     2461 ataagaaaag ccgctcgaac ctgagcggct tttcacttta atccgacctt ac
//
LOCUS       DASWNS010000048         9191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_27455, whole genome shotgun sequence.
ACCESSION   DASWNS010000048 DASWNS010000000
VERSION     DASWNS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27455"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VGB76_01655"
     CDS             <1..1204
                     /locus_tag="VGB76_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY0097633.1"
                     /translation="AEPIFKPGTADGYKFFLEMGSLANVNEKFYKGQVGFWNEMLAHP
                     NYDAWWKARNVPQHLSGIRPAVMTVGGWFDAEDLYGALKTYEAIERKNPGIPNTLVMG
                     PWLHGGWAGMDGDVLGDINFGEKTGLFYREKIEFPFFQCVLKDRCEEKLPEAYIFETG
                     SNRWRRFDSWPPKNAKEVSLYLDAKGRLSFNPATGAALAFDEYVSDPARPVPHLNRIT
                     LGMTGDYMIQDQRFASRRPDVLVYQTEPLKEEITLAGPVAADLVVSTTGTDSDFVVKL
                     IDVFPDNTPDPNPNPKNLRMGGYQMLVRGEPMRARFRNSWERPEALTPNQPTKVAFVM
                     PDTSHTFRKGHRIMVQVQSSWFPLVDRNPQKFVNIYQARESDFQKATQRIYRAGRMAS
                     RLKLTLLP"
     gene            complement(1328..1579)
                     /locus_tag="VGB76_01660"
     CDS             complement(1328..1579)
                     /locus_tag="VGB76_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097634.1"
                     /translation="MTAREPALAALEDELSAVRDEGGNSFCANKYWYVRVAPRVAGLV
                     GAQAAVDDSLLRSPEAYTLAYEVLYRLLPNCRNCVCVPW"
     gene            complement(1612..2307)
                     /locus_tag="VGB76_01665"
     CDS             complement(1612..2307)
                     /locus_tag="VGB76_01665"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEY0097635.1"
                     /translation="MALPARYRGAGYRAWMSEDLGALVESKDLTLSHLQKGYVRSRWL
                     QQVLRLDYELQRALKRYQVLRLATALGCLVILMLVSLGFDEARWGTWSNGVRYLTILS
                     TLLVAASVVVEHLFNYGERWRETQQHLERLQTEGWRFLQLSGHYRHYENHAAAFAAFV
                     NQVEELSQREVEVYSSRVMHERRKGGGAGESEQAKKSETMPAETPEPRPDVPTVPMRD
                     QIAPRRVQLPRGQ"
     gene            2739..3554
                     /locus_tag="VGB76_01670"
     CDS             2739..3554
                     /locus_tag="VGB76_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097636.1"
                     /translation="MKKSKLGALALAVVLTLSLSAGILADTIRLRDGSVVRGQIIAFK
                     DQQFTVLIGAGTRGRRGRMTIYSEDIESIEFDAAGGAADAGGASSGGVDTSNDDRSGS
                     VNTAPSTRNPPADPVRPRTDTPPASDRDADAGTANPGGSPIYFPIRLRVRADNASNGW
                     NDSGLMVRAGQRLRITATGRVSLGNGQMSTPTGLPRLADRDKLMRNEPTGGLIAVIGD
                     DNDDFIFVGANREFVAQRAGRLFLGVNEGNLGDNSGTYDVTVEAEAVSSTGRP"
     gene            3750..4994
                     /locus_tag="VGB76_01675"
     CDS             3750..4994
                     /locus_tag="VGB76_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0097637.1"
                     /translation="MKNSLTYRRVVALAVAFMFIFANVAVTSVATRAQSRRKPETLPQ
                     KKNQRPGEQKPDEEKPAEEVPKEVINTPQEAEVVKVVTNLVNIETTVVHKKSKQIVAN
                     LKQANFAVFEDGVQQVITNFATPEAKLNVAVVIEYSKLAQSISSIGTSGMEDGRRELI
                     APAALFMQDAVQRGDYISVIAYDMRPTPLTDFTNDPRRVSEVINLLVRNRPAFRETNL
                     FDALKLTIAGGRADSVVLEDNQPEKSDYSGLASVEGRRRAIFLISTGIDTFSKTNYDQ
                     ARKIAQNAGIPIYIIGTGNLFFKKYSDQMDAVDSLTGFPGRLTMIQAQNTLSTFAKET
                     GGAYFPVTFEGELRSTLSSINALMRSQYSLGYNPGERRDGKQHKIVVKVDINGDGVFD
                     EKEFDVQHRKFYNSPKPEKSDE"
     gene            complement(5103..6401)
                     /locus_tag="VGB76_01680"
     CDS             complement(5103..6401)
                     /locus_tag="VGB76_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HEY0097638.1"
                     /translation="MARRKYRSTQPVTERSFLEYLSTPALQTSRIELLRLVRGGEDTF
                     LELKVKLSNPEKIAQEIVALANTGGGVMVFGVSDQLRVEGVDDPEDVRDEIVRICREQ
                     IAPQLVPYIDLIAFDNGRRVVAVEIEGKRRPYQTRGDGRYFIRIGADKREASREELSA
                     LLDEARPLRYENVTVPGASDADIDEAHLWSFVREFAGDAFDSDLNARDYPTADVLKRD
                     LLLAAGNMGESVPTVAGLLLFGRDERVAALLPRSVVTAIRYAGDTVQDAEIERVELKG
                     NLHTLYESSLRFIARYCDLLDARPRTRHAPPEDAPVAARANYQRAAVGEAVANALVHR
                     DLALRDHSTRILVFDHSIEILNPRRTIGFAPPAQRAIRYGVPQQLNPQIAAIFRNPAY
                     GLALPHGGLPMLLRQTRLFSNRRADINLFNDEFRLRLYGI"
     gene            complement(6573..8294)
                     /locus_tag="VGB76_01685"
     CDS             complement(6573..8294)
                     /locus_tag="VGB76_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097639.1"
                     /translation="MTGNSNTAQLGALVWLKWRLFLNAMRSKRGAANRVASILGTLAA
                     LAISLLIAAGFGAGAYFVIANRTSGGRRAATSMARDLATGASVEALLFLFLFMSMIYL
                     MWAFVPLSLGGGEHFDPGRLLLYPISLRKLFLIDLLSELTSLASIFAAPSVFAIALGA
                     GLARGQVGSALVVAVCATLFGIALAKLLATAVGALMQRKRTRGEAVLAFLGVLGALSG
                     VLLGQAEHLFGRLENFPAALRWTPPGAVAFALAAPFAGAHYALSLAVLLAYTFVLTAL
                     TYKIARGALAGDSGGKRRRKAATAANAGAGATETRKAFGWRLPFGSEELSAIFEKELR
                     YALRNAQLRTMALMPIILTISFRLLGTRRGGAFGGALPESIAPYMQGSGAALSVLYAF
                     LITSALSSNLFAYESGGMRAYVLAPVARSRILLGKNLAMLVVALGFALAVTLVNALVY
                     RDLTPRALLFTALSFVFFAAATATIGNLFSINFPKRLEFGKRMNASGVAGVLLLPVFM
                     CIAAPPALAVLAGYAAQSLFVEYVILAAFAVAGVAVYFLSLKAQGRLLARRELDILEA
                     VTGRGDD"
     gene            8438..>9191
                     /locus_tag="VGB76_01690"
     CDS             8438..>9191
                     /locus_tag="VGB76_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097640.1"
                     /translation="MSNLLRTPSLLLVVILAAAVGLAQEKKQEKKKKKDEPTVTARVL
                     TEPLPAEYDPAAWKVYKSEAGRFSVLFPGTPQEETDKLQSGVHEVPIHILKFNALAAY
                     ALMYLDSPAAFDLPPEATRKMLDAHAKQAIATFQATTLQQKEVALDGHPGLAYMVRLP
                     DGLIMRLRVYAVRSRLYQLMIVTPPEQGATDGQRRFYEATADKFLDSFKLTPLAEPAP
                     VVSVQDAPEPPAPKRLPHAPVSGGVLNGKAVSK"
BASE COUNT         1857 a         2956 c         2794 g         1584 t
ORIGIN      
        1 ggctgaaccc attttcaaac ccggcacggc tgacggctac aagttcttcc tcgaaatggg
       61 atcgctcgca aacgtcaacg aaaagtttta caaagggcag gtcggcttct ggaacgagat
      121 gctggcgcac ccgaattacg acgcgtggtg gaaggcgcgc aacgtcccgc aacacctgag
      181 cggcatccgc ccggcggtga tgaccgtcgg cggctggttc gacgccgaag acctctacgg
      241 cgcgctcaag acctacgagg ccatcgagcg caaaaacccc ggcatcccga acacgctcgt
      301 catgggaccg tggctgcacg gcggctgggc gggcatggac ggcgacgtgc tcggcgacat
      361 caacttcgga gagaagaccg gcctgttcta tcgtgagaag atcgagtttc ccttcttcca
      421 gtgcgtgctg aaagacaggt gcgaagagaa gttgccggag gcttacatct ttgaaaccgg
      481 ctcgaaccgc tggcgcaggt tcgacagttg gccgccgaag aacgcgaaag aggtgtcgct
      541 ctacctcgac gcgaagggca ggctctcctt caacccggcc accggtgccg cgctcgcctt
      601 cgacgagtat gtgagcgacc cggcgcgtcc cgtgccgcac ctcaaccgca tcacgctcgg
      661 catgacgggc gactacatga ttcaggatca gcgtttcgcc tcacgccgtc cggacgtgct
      721 cgtctatcag accgaaccgc tcaaagaaga gatcacgctc gcgggtcccg tcgccgccga
      781 cctcgtcgtc tcgacgacgg gcacggattc ggatttcgtc gtcaagctca tagacgtgtt
      841 tccagataac acgcccgacc ccaatccgaa cccgaagaac ctgcgcatgg gcggctacca
      901 gatgctcgtg cgcggcgagc cgatgcgcgc gcgcttccgc aacagttggg agcgaccgga
      961 agccttaacg ccgaatcaac cgaccaaagt tgcgttcgtg atgccggaca cgagccacac
     1021 cttccgcaaa ggccatcgca tcatggtgca ggtacagagc agttggtttc cactcgttga
     1081 ccgcaacccg cagaagttcg tcaacatcta ccaggcgcgc gagtctgact tccaaaaggc
     1141 gacgcagcgc atctatcgcg cgggacggat ggcttcacgt ttgaagttga ctctcctgcc
     1201 gtagcgtaaa agggatgagg gcgaagggat gaaggatgag ggatgaggga taaatcagag
     1261 aaagcattca agcgagtgag tgcttcctct gattcatccc tcatccctca tccttcatcc
     1321 cttttttcta ccacggcacg cagacgcagt tgcggcagtt tggcaggagg cgatagagga
     1381 cttcgtaggc gagcgtgtag gcttcgggcg aacgcaggag ggaatcgtca acggcggcct
     1441 gcgcgcccac caaccccgcg acgcgcggcg cgacgcgcac ataccaatac ttattcgcgc
     1501 agaaagagtt gccgccctcg tctctcaccg ccgacaactc atcttccaac gcggcgagcg
     1561 cgggttctct cgccgtcaat atctcccacg tgataaccgg ggcgagataa tttactgccc
     1621 ccgtggaagc tggacgcggc gcggggcgat ctggtcgcgc atgggaaccg ttggaacgtc
     1681 cgggcgcggc tccggcgtct cggcgggcat cgtctccgac ttttttgcct gctccgactc
     1741 gcctgcgcca cctccctttc tccgctcatg catcacccgc gagctataga cttccacttc
     1801 acgctggctc aactcttcca cctgattgac gaaggccgcg aaagccgccg cgtggttttc
     1861 gtaatggcga tagtggccgc tcagttggag aaaacgccag ccctcggttt gcagtcgttc
     1921 cagatgttgc tgggtttcgc gccaacgctc tccgtagttg aagagatgct cgacgacgac
     1981 gctggccgcg acgaggagcg tcgagagaat cgtcaggtag cgcacgccgt tcgaccaagt
     2041 gccccaacgc gcttcatcga atccgagact gacgagcatc aggatcacga ggcagccgag
     2101 cgcggttgcc agacgcaaaa cctgatacct cttgagcgcg cgctgcaact cgtaatccag
     2161 acgcaagact tgttgtagcc agcgcgaacg cacgtagccc ttttgcaaat gggacagcgt
     2221 caaatccttc gactcgacga gcgcgcccaa atcttcgctc atccatgcgc gatagcccgc
     2281 gccccgatac ctcgccggta aggccatacc ttaaactccc ccttaggtgg tcaatgcgtg
     2341 ttgaagatgt ggtaaataaa gctgctcccc gccggtcgcc gccgctgtag gctctggcaa
     2401 atgcctgcgc gcatttcgcc attgtaggtt tccgaacatc aaagattgac tttaaactca
     2461 gttgaagact gagagaatag ccgagaacca aagagagtga caagtgacaa gtgacgggtg
     2521 acaagttaaa actaaatcct cgtctttaaa cttgtctttg acccgctcgc ctgtcacccg
     2581 ccacttgtca ctcgtcacgg ttttacaggc aacccgcgcg tgcgattcgg acatcctttc
     2641 gtgcaacatg ctactctccc gcttgccaaa gcgtgtctcg gtttggtaaa cgtccgctta
     2701 acaattcgtc ggttgtccga gagtttccgg gagggattat gaagaagagt aaattgggcg
     2761 cgctcgcgct ggccgtcgtc ctcacgttga gtttgagcgc gggcatcctc gccgatacga
     2821 ttcgcctgcg cgacggctcg gtcgtgcgcg gccagatcat cgcctttaaa gatcaacagt
     2881 tcacagtgct catcggcgcg ggcacgcgcg ggcggcgcgg gcgcatgacg atctactcgg
     2941 aagacatcga gtccatcgag ttcgacgcgg cgggcggcgc agccgatgcg ggcggcgcgt
     3001 cgagcggcgg cgtggatacg tcgaacgacg accgctccgg cagcgtcaac accgcgccct
     3061 ccacacgcaa tcctcccgca gaccccgtgc gcccccgcac cgacacgccc ccggccagcg
     3121 accgcgatgc ggacgcgggc acggcgaacc ccggcggctc gcccatctac ttccccatcc
     3181 gcttgcgcgt gcgcgccgac aacgcctcga acggctggaa cgacagcggc ctgatggtgc
     3241 gcgccggtca acgcctgcgc atcaccgcga cggggcgcgt ctctctcggc aacggccaga
     3301 tgtccacgcc gacgggtctg ccgcgtctcg cagaccgcga caaactgatg cgcaacgagc
     3361 cgacgggcgg cctgatcgcc gtcatcggcg acgataacga cgacttcatc ttcgtgggcg
     3421 cgaaccgcga gtttgtcgcc cagcgcgccg ggcggctctt cctcggcgtc aacgaaggca
     3481 atctcggcga caactccggc acttatgacg tgacggttga agccgaagcc gtctcctcca
     3541 ctggccgccc gtaagcgact cgtcccgaaa cgcgtgcgaa gcgcgagatg ccgacggctg
     3601 ctccgcaggc ggctggcggc atctcgcgca cgcgtccccg catctaatcc tttcttgctc
     3661 tatcagcggc gtgcgccgta atattgggtc agcgcagaca acttgtcttt cacccgccct
     3721 gtcagtcttt ttaagaggtt tgtattgcca tgaagaactc tctcacctat cgtcgcgttg
     3781 ttgctctcgc cgtcgcgttc atgttcattt ttgccaacgt cgccgtgacg agcgtggcga
     3841 cgcgcgcgca atcccgacgc aagccggaga cgcttccgca gaagaagaac cagcgaccgg
     3901 gcgagcagaa gccggacgaa gagaaacccg ccgaagaggt tccgaaagag gtcatcaaca
     3961 cgccgcagga ggcggaggtc gtcaaggttg tgacgaacct cgtcaacatc gaaaccaccg
     4021 tcgtccacaa gaagagcaaa cagatcgtcg ccaacttgaa gcaggccaac ttcgccgtct
     4081 ttgaagacgg cgtgcagcag gtgatcacca acttcgccac gcccgaagcg aaactcaacg
     4141 ttgccgtcgt catcgaatac agcaaactcg cgcagtcgat cagttccatc ggcacgagcg
     4201 gcatggaaga cggtcggcgc gaattgatcg cgcccgccgc gctcttcatg caggacgcag
     4261 tgcagcgcgg cgactacatc tccgtcatcg cctacgacat gcgcccgacg ccgctcacag
     4321 actttacgaa cgacccgcgc cgcgtcagcg aagtgatcaa cctgctcgtg cgcaaccgtc
     4381 cggccttccg cgaaacgaac ctcttcgatg ctttgaaact cacgattgcg ggcggtcgtg
     4441 ccgattccgt cgtgctcgaa gacaatcaac cggagaagtc ggactatagc ggcctcgcca
     4501 gcgtcgaagg tcgccgccgc gccatcttcc tcatctccac cggcatcgac acgttcagca
     4561 agacgaacta cgatcaggcg cgcaagatcg cgcagaacgc cggcatcccg atctacatca
     4621 tcggcacggg caatctcttc ttcaaaaaat acagcgacca gatggacgcc gtagactcgc
     4681 tcacgggctt ccccggtcgc ctcaccatga ttcaggcgca gaacacgctc agcaccttcg
     4741 ccaaagagac aggcggcgca tacttccccg tcaccttcga aggcgaactc cgctccacgc
     4801 tctcatccat caacgcgctt atgcgcagcc agtacagcct cggctacaac cccggcgaac
     4861 gccgcgacgg caagcagcac aagatcgtcg tcaaagttga catcaacggc gacggggtct
     4921 tcgacgaaaa agagttcgac gtgcagcacc gcaagtttta caactcaccc aagcctgaaa
     4981 aaagtgacga gtgacaggtg acgagttaaa gctcacatct cgctcgcaga ttgatgaaag
     5041 ccgtgcatcg taaaatacga tgcacggctt ttcacttatc actcgtcact tctcactgcc
     5101 tttcagattc cgtagaggcg caagcggaac tcgtcgttga agaggttgat gtcagcgcgg
     5161 cggttggaga agagccgcgt ctggcgcagg agcatgggga gtccgccgtg cgggagggcg
     5221 aggccgtagg cggggttgcg gaagatggcc gcgatttggg ggttgagttg ctgcggcacg
     5281 ccgtagcgga tggcgcgctg tgcgggcggc gcgaagccta tggtgcggcg cgggttcaaa
     5341 atctcgattg aatgatcgaa gacgaggatg cgcgtcgagt gatcgcgcag ggcgaggtcg
     5401 cgatgcacga gcgcgtttgc gacggcttcg ccgaccgccg cgcgctggta attggcgcga
     5461 gccgcgacgg gcgcatcttc cggtggcgcg tggcgcgtgc gtggccgcgc gtcgagcagg
     5521 tcgcagtagc gcgcgatgaa gcgcagggag gattcgtaga gcgtgtgcag gttgcctttg
     5581 agttcgacgc gctcgatctc tgcgtcctgc acggtgtctc cggcgtagcg gatggccgtg
     5641 acgacggagc gcggcaggag cgcggcgacg cgttcgtcgc gcccgaagag gaggagtccg
     5701 gcgacggtcg gcacggactc gcccatgttg cccgccgcga gcagcaggtc gcgcttcaac
     5761 acgtctgcgg tcgggtagtc gcgcgcgttc aaatccgaat cgaaagcgtc tccggcaaac
     5821 tcgcgcacga agctccacaa gtgcgcctcg tcaatatccg catcgctcgc gcccggcacg
     5881 gtgacgttct cataacgcag gggtcgcgct tcatcgagca gcgcggagag ttcttcgcgc
     5941 gaggcttcgc gcttgtccgc gccgatgcgt atgaagtaac gcccgtcgcc gcgcgtctgg
     6001 taggggcgac gcttcccttc gatctccact gcgacgacgc gccgcccgtt gtcgaacgcg
     6061 atgagatcaa tgtaggggac gagttgcggc gcgatctgtt cgcggcagat gcgcacgatc
     6121 tcgtcgcgca cgtcttccgg gtcgtccacg ccttcgacgc ggagttgatc gctcacgccg
     6181 aagaccatca cgccgccgcc cgtgttggcg agcgcgacta tttcctgcgc gatcttctcc
     6241 gggttcgaga gtttaacttt gagttcgaga aaggtgtctt cgccgccgcg cacgagccgc
     6301 aggagttcga tgcgggaggt ttgcagggcg ggcgtggaga gatactccag aaaagagcgt
     6361 tccgtgacgg gctgggtgct gcggtatttt ctgcgtgcca tttgtgtctg gacggggacg
     6421 catccacctt tggactagcc cgcccggatg ccgcgcgccg gatgaaacga taaatgcgcg
     6481 cgccgcacct ctggaatcaa tattaacttc aagcgacgac cggaaaagtt tccgcacggg
     6541 cggtctgtcc gcgcggagag agaggcggcc tgtcagtcgt cgccgcgtcc ggtcacagct
     6601 tcaaggatgt caagttcgcg cctcgcaagc aggcgtccct gcgccttgag cgacaaaaaa
     6661 tagacggcca cgcctgcgac ggcgaacgcc gcgagtataa catattcgac gaagagactc
     6721 tgcgcggcgt agcccgcgag caccgcgagc gcgggcggcg cggcgatgca catgaagacc
     6781 ggcagcagga gcacccccgc cacgcccgac gcgttcatac gcttcccgaa ttcgagtcgc
     6841 ttcgggaaat tgatcgagaa cagattgccg atggtcgccg tcgccgccgc gaagaagacg
     6901 aaggagagcg cggtgaacag gagcgcgcgt ggcgtcaggt cgcggtagac gagcgcgttg
     6961 acgagcgtga ccgcgagcgc gaagccgagc gcgaccacga gcatcgccag attcttcccc
     7021 agcagaatcc tgctccgcgc gacgggcgcg agcacgtaag cgcgcatccc gccagactcg
     7081 tacgcgaaga gattcgacga cagtgcggac gtgatcagga aagcatagag cacgctcaag
     7141 gccgcgccgc tgccctgcat gtaaggcgcg attgactctg gcagagcgcc gccaaacgcg
     7201 ccgccgcgcc gcgtgccgag caacctgaac gagatggtga ggatgatcgg catcaaggcc
     7261 atcgtgcgca attgcgcgtt gcgcaacgcg tagcgcaact ccttttcgaa gatcgcggac
     7321 aactcctccg agccgaacgg cagacgccac ccgaacgctt ttcgcgtctc cgtcgcgccc
     7381 gctccggcgt ttgccgccgt agctgccttc cggcgtctct tcccgccgct gtctcctgcc
     7441 agcgcgcccc gcgcgatctt gtaagtcaga gccgtgagga cgaaggtgta agccaggagc
     7501 actgcgagcg agagcgcgta gtgcgcgcct gcaaagggcg cggcgagggc gaaggccacc
     7561 gcgccggggg gcgtccagcg caaggctgcg ggaaaatttt cgagccgccc gaaaaggtgt
     7621 tcggcctgtc cgagcaggac gccggagagc gcgccgagca cgccgaggaa agccagcacg
     7681 gcctcgccgc gcgtccgctt cctttgcatc aacgcgccga cagccgtagc gagcagtttc
     7741 gcgagcgcga tgccgaagag cgtggcacag acggcgacga cgagtgcgct gcctacttgc
     7801 ccgcgcgcta gccccgcgcc gagcgcgatg gcgaagaccg acggcgcggc gaagatcgag
     7861 gcgagactcg ttaactcgct caggagatct atgaggaaaa gtttgcgcag gctgatcggg
     7921 tacaacagga gcctgccggg atcgaaatgc tcgccgccgc cgagactcaa aggcacgaac
     7981 gcccacatca gatagatcat ggacatgaac aggaacagaa agagcaacgc ttcgacgctc
     8041 gctcccgtcg cgaggtcgcg cgccatgctt gtcgctgcgc gcctgccgcc gctcgtccgg
     8101 ttggcgatga cgaagtacgc gcccgcgccg aagcccgccg cgatcaagag cgagatggcg
     8161 agcgcggcga gcgtgcctaa aattgacgcc acgcggttgg ccgcgccgcg cttcgagcgc
     8221 atcgcgttca ggaacagacg ccacttcaac cagacgagcg cgcccagttg cgccgtgttc
     8281 gagttgcctg tcatccgccg cctccccttt tctttccctg ctgctgcgcg cgtggcggtt
     8341 cgcaccgcaa agcgatacac tgcaacgcgt cgccggattt aaccacagtt cgccgcgccg
     8401 cacacacgat tcgcttcact tgaaaggact cccggttttg tcgaacctct tacgcacccc
     8461 gtcgctgctc ctcgtcgtaa ttctcgccgc cgccgtcgga ttggcgcaag agaagaagca
     8521 ggagaagaag aagaagaagg atgagccgac cgtgacggcg cgcgtcctca ccgaaccgct
     8581 gcccgccgag tacgacccgg cggcgtggaa ggtctacaag tctgaggcgg gacgtttcag
     8641 cgtcctgttt cccggcacgc cgcaggaaga gacggacaag ctgcaaagcg gcgtccacga
     8701 agttcccatc cacattctca aattcaacgc cctcgcagcc tacgccctca tgtacctcga
     8761 cagcccggcg gcgtttgacc tgccccccga agcgacgcgg aaaatgctcg acgcacacgc
     8821 gaagcaggcg attgcgacct ttcaggccac cacgctccaa cagaaggagg tcgcgctcga
     8881 cggccatcca gggcttgctt acatggtgcg cctgccggac ggactgatca tgcgcctgcg
     8941 cgtctatgcc gtccgctcgc gcctctatca acttatgatc gtgacgccgc ccgaacaggg
     9001 cgcgaccgac ggccagcgac gcttttatga agcgaccgcc gacaagtttc tcgactcctt
     9061 taaactcacg cccttggccg aacccgcgcc cgtcgtgagc gtgcaggacg cgccggaacc
     9121 gcccgcgccg aagagacttc cccacgcccc tgtgagcggc ggcgtgctca acggcaaggc
     9181 cgtctcaaaa c
//
LOCUS       DASWNS010000049        11411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_339065, whole genome shotgun sequence.
ACCESSION   DASWNS010000049 DASWNS010000000
VERSION     DASWNS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339065"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..226)
                     /locus_tag="VGB76_01695"
     CDS             complement(11..226)
                     /locus_tag="VGB76_01695"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY0097641.1"
                     /translation="MKITEEFVEMMICPACRSKVRLKEDGSAIKCVGCRRVYPIEDDM
                     PMMLPDKATIEDEETTPTTTTDAPRPA"
     gene            complement(361..1740)
                     /locus_tag="VGB76_01700"
     CDS             complement(361..1740)
                     /locus_tag="VGB76_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0097642.1"
                     /translation="MATAIPTPGGARPKRTGSPLFDLLAFRRDPLKFLTRLAREHGDI
                     VPFRMGPQRVLLLNHPDLVRDALVTRADHFHKGRALQRAKRLLGEGLLTSEGEHHRRQ
                     RRLAQPAFHRKRVDSYGAVMVDYAARDSDARWHDGATLDLSHEMMRLTLAIVGKTLFD
                     ADVESDTDEIGSALTELLELFQMLLLPYSEYLERLPLPANRRFTRARDKLDAVIYRII
                     DERRAGGADRGDLLSMLLLAQDEEGAGGGMTDRQLRDEALTIFLAGHETTANALAWTW
                     YLLSQNPEVEERLHAELDAVLDGGRLPTVEDLPQLRYTEMVLAESMRLYPPAWVVGRL
                     AVKEYAIDDYLAAEGTLVLISQYVLHRDPRFFPDPLRFDPERWTTEAKEARPPYAYFP
                     FGGGARRCIGEGFAWMEGTLIIATIARRWRMRLVPGHAVVPHPRMTLRAKHGIRMRME
                     DRAKGKGER"
     gene            complement(1816..2133)
                     /locus_tag="VGB76_01705"
     CDS             complement(1816..2133)
                     /locus_tag="VGB76_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097643.1"
                     /translation="MRSNVQAYNNKETAQALDGVPGGAKPGGKVLMSKTAKKLMMCAL
                     SLYAGSFLSCYIAARPRQPAPGEVWASGFDTIGWYDFGFALLIVAGGFTLAAIRFSYH
                     RTG"
     gene            2197..2958
                     /locus_tag="VGB76_01710"
     CDS             2197..2958
                     /locus_tag="VGB76_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097644.1"
                     /translation="MSLEFAIEYPCQLRARYTEEHLRAWGRLGNIHTRVTTGEQAAPS
                     EEKLRFVERSSIEIERMQEETSVCEKCPARLPHDMAGEGEAVGCQGRITYPFDAQFEK
                     FLADRVQLALDTVSLEDQPRLLRILVDPDSPFDGEGAKSLRAVTTPEGLRFFELRLPI
                     RLAREASHVTTDHLFDALAGFTAEDNARTSYARELPPKAAPDYYDFLDLILLNDLSGS
                     ERARIEKRSHNYQQFLRLHAALERAEALQARILLD"
     gene            complement(3046..3639)
                     /locus_tag="VGB76_01715"
     CDS             complement(3046..3639)
                     /locus_tag="VGB76_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097645.1"
                     /translation="MADLTLDGCWNSSVCGSSSPVLIDVAGDGFNLTDAPHGVAFDLN
                     NDGHREWLSWTAVDSDDAWLALDRNGNGQIDNGGELFGNFTLQLWSATPNGFLALARF
                     DRPEKGGNADGVIDGGDGVFADLRLWQDRNHNGLSEAAELQTPASLNVAALHLEYKES
                     KRTDEHDNHFRYRARVRDAGGAQVGRWAWDVFLVRGN"
     gene            complement(4415..5128)
                     /locus_tag="VGB76_01720"
     CDS             complement(4415..5128)
                     /locus_tag="VGB76_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097646.1"
                     /translation="MSYKSITIICSFVLLACMGIGYIKTSSKNKVRQVPIESEKRGTL
                     RQQIAKAKSNGQLELNLSGLIANYEQVKNLDDALTRYTVLIAQPVEERSYLNEHDSIT
                     TWYKFKILEPVSQPAVAKSFDFIQPPVELLPLNDDEILVPRIGGTLTVDGVKVTRSEH
                     IFPTFKKSRKYLIFVLLDNSIKVGLPDLGPAGVLTINDDNSLTPINDVTLPLEKELKA
                     RYGKSLPEIRERFATARSR"
     gene            complement(5254..5628)
                     /locus_tag="VGB76_01725"
     CDS             complement(5254..5628)
                     /locus_tag="VGB76_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097647.1"
                     /translation="MHRHRKILLLLVVLSVTALSALTALSGQEQTSAAAPTQHSAAEA
                     PEPVADYAAGEPADPEKRAKRRARGKSYDGQSWVMEPHPQSRRQLLYVGAPLAACLLT
                     LSVRARSEPPRSFKDEIEEVLV"
     gene            complement(5849..6574)
                     /locus_tag="VGB76_01730"
     CDS             complement(5849..6574)
                     /locus_tag="VGB76_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0097648.1"
                     /translation="MKDAHTDTGYSEDVETALLDVELFLKYRSHTRAMARLQEAIKSN
                     PQAIKLRERLREVALANGQPEEAARQCLALTNLYITRDNFDAAQERLLEAKQTDPRIN
                     IVPGLEAIRRARRPDLPPAPSAATNAPQRAPRALTFAGDLSVVSIFDAVQVIENARLT
                     GTLTLSGGGAQGGHIRFNEGRIAAAESGVERDTDALRRLVEMTGGEFDFERSTGSYPT
                     TIQATSNTSLLLNALRELDEENR"
     gene            7067..7759
                     /locus_tag="VGB76_01735"
     CDS             7067..7759
                     /locus_tag="VGB76_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0097649.1"
                     /translation="MQEELTILDASAAATVRLLNNSWANYLQRACTRLKSVGQINQTN
                     GTHALVSRLRRTRPSTYEHSVRVARLARVTGRAFNFTDTRLQQLSSTAMLHDIGKILV
                     PEAVLNRPRRPTRMEQFVLHLHPSLGALLASYFNLSPELRIRTQHHHERWDGKGYPHR
                     LGGEDIPLMARIVQVVDTYDAMVARRPYSNPRTHNEAVAELLAHGGKQFDPRVIEAFL
                     DAHPAHLDETGF"
     gene            complement(7852..11328)
                     /locus_tag="VGB76_01740"
     CDS             complement(7852..11328)
                     /locus_tag="VGB76_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0097650.1"
                     /translation="MRPRTLHTISHVCLWQICPCLFALLLSPLAPAHYGVGAAFAQDL
                     DDVVISGRVIDQNGAGIAGAKVSVVHAATNFARSVVADSEGRYRLVELAPGVYTLRAA
                     CEGFATQERAGVETLAGQRVQLDLTLRPAGVAAEQVVLSEASGNVVDTTRTVVGGTLT
                     RAELETLPSPTRSPLDLIYTLGGVSEEPLSTRDVAEDRNRTPARTPEEAGTFALSGGA
                     AYSNNITIDGLDNNDDRAARERFQPSLEAVAEVQVITNQFSAEYGRASGGRVNLRTRG
                     GAHDFHARAFYFFRDESLNANSYFNNARGLKRLPLQEHAPGFTLSGQLKSPLKLFGAR
                     DRAGAPDGVRAIATESGGADGNRATRPSRHHFFIAHESQIVLDATVIDTLVPVRANPR
                     FPLPAPTTLAGRRTEASSAAPFEPAELAPYVEHTATPLRAYNFTARTDHNFSDAHNAT
                     FLYQRGRSRNLRQFGGGTRLADALQGRTRHSDALAYTDNFVGSPRLVNQLRAQISRLT
                     PALRTADSQARPVVLISIDDPLAAVSTADRSGTLVAGSSNAGASDRRELRVQLQNTLT
                     VLRGAQTFKFGGDLQGIRSTFIDLSDASGTFDFESAGEFLADAPSRFRQRFNGESEQK
                     NLYTGFFAQVEWHARPNLTISYGLRYENETILKDRNNFAPRLALALDPFRTGKTVIRL
                     GAGLFYNRVLLRTVDDFTLGRARIFFDTDTLRSPASGRLMTDAERRAFIAANLRFPET
                     LNAGSPIVKQFGALQSNFTRRLDPDLRIPESYQANVGFERELARGFVVEANYTFNRGL
                     HLWREFNANAPRLPHGYRDFAAYLLSRDFANFRDVSGTRPLYNTQSAGELVRFTLAAP
                     GAPNPDAIARVVEFGVPVSIFNLDSFSSSVALEAALAALNDLRPDPARGQIEQLTSIG
                     NSFYHGLTVEARRRFARREQGFGLSLRAAYTLSRLIDDGVVNTSSALTPGDFRAERAP
                     GLLDRRHRFALSGTFDTPRKLGALRFATILRVASGAPFNLSLGGADRNLDDVSNDRPV
                     FNGDPRHIRSRRPGEPLNPELPGAFSLPIIGRNGNLPRNAGRGDALFTFDLNITREFR
                     FGERLSLRASIEIDNPLNKTVYTFGAEFINFNALRPTATAAQRQAFLDSFLVPTHTLR
                     PRSVRVGLRFDF"
BASE COUNT         2181 a         3317 c         3574 g         2339 t
ORIGIN      
        1 agcgttcatc tcaagcgggg cggggagcgt ctgtcgtagt agtcggggtc gtctcttcgt
       61 cctcaatcgt ggccttgtcg ggcagcatca tcggcatgtc gtcctcaatc gggtagacgc
      121 gcctgcaacc cacacacttg atcgcgctgc cgtcctcttt gagccgaacc ttcgaccggc
      181 aggccgggca gatcatcatc tcgacaaact cttccgtgat cttcatgcgc ccaagattag
      241 caaaggcgac gggggaaacg aaactaaggg caaaggcgac gggaacgagg ggtagagaga
      301 aagggggaag gataaagatc agaagcgcgt cccgccggtt tcgttttgcg ccttcccttt
      361 ttacctttcc cccttcccct tcgccctgtc ttccattctc attctgattc cgtgcttcgc
      421 gcgcagcgtc atgcgcgggt gcgggacgac ggcgtgaccg gggaccaggc gcatccgcca
      481 gcgacgcgcg atggtggcga tgatgagcgt cccttccatc cacgcgaagc cctcgccgat
      541 gcagcgacgc gcgccgccgc cgaaagggaa ataggcataa ggcggcctcg cctctttcgc
      601 ttccgtcgtc cagcgttcgg gatcgaagcg cagggggtct ggaaagaagc gcgggtcgcg
      661 gtgcagcacg tactggctga tgagcacgag cgttccctcc gccgcgaggt aatcgtcaat
      721 cgcatactcc ttgaccgcga gccgaccgac gacccacgcc ggaggataga gccgcattga
      781 ctcggccaag accatctccg tgtaacgcaa ctgcgggagg tcttcgactg tcggcaggcg
      841 tccgccgtcg agcaccgcgt cgagttccgc gtgcagtctc tcttcgacct ccggattttg
      901 cgagagcaga taccacgtcc acgcgagcgc gttggcggtc gtctcgtgtc cggcgagaaa
      961 gatggtcaac gcttcgtcgc gtagttgccg gtcggtcatg ccgccgcccg cgccctcttc
     1021 gtcctgcgcg agcagaagca tcgagagcag gtcgccacgg tctgcgccgc ccgcgcgccg
     1081 ctcgtcgatg atgcgataga tgaccgcatc gagtttgtcg cgcgcacgcg tgaagcgacg
     1141 attggcgggg agcggcaggc gttcgagata ctcggaatag ggcagcagga gcatctggaa
     1201 gagttccaat agttccgtga gcgcgctgcc gatctcgtcc gtgtcggatt cgacatcggc
     1261 atcgaacaga gttttgccga cgatggcgag cgtgagtcgc atcatctcgt gcgagagatc
     1321 gagcgtcgcg ccgtcgtgcc agcgcgcgtc gctgtcgcgt gcggcgtagt caaccatcac
     1381 cgcgccgtaa ctatcgacgc gcttgcgatg aaaggcgggc tgcgcgaggc gacgttggcg
     1441 gcgatggtgt tcgccctcgc tcgtcaggag tccctcgccg agcagacgct tggcgcgttg
     1501 gagtgcgcgc cccttgtgaa aatggtctgc gcgcgtgacg agcgcgtcgc gcacgagatc
     1561 gggatgattg agcaggagga cgcgctgtgg tcccatgcgg aaagggacga tgtcgccgtg
     1621 ttcgcgtgcg aggcgcgtga gaaacttcag agggtcgcgg cggaaagcga gcagatcgaa
     1681 gagcggcgag cctgttcgtt tcgggcgcgc gccgccgggg gtcgggattg cggttgccat
     1741 cagtggaaac tccgccggtc agattcgacc cgccgccgcg cgtgcgggaa acgactttac
     1801 agttcgcaat ccttgtcagc cggttctgtg atagctgaac cggatcgcgg ccagagtaaa
     1861 accgcccgcc acgatcaaga gcgcgaagcc gaagtcgtac caaccaatcg tatcaaatcc
     1921 cgaagcccac acctcgccgg gcgcgggctg gcgcggacga gcggcgatgt aacaactcag
     1981 aaaagaaccg gcgtagagcg acagggcgca catcatcaac tttttagcag ttttgctcat
     2041 cagcactttt ccgccgggtt tcgcgccgcc gggaacgccg tctaacgctt gcgccgtctc
     2101 tttgttatta tacgcctgca cgttcgaacg cacgtagaaa agagcgcgat tctttaggga
     2161 gaatcggggt gcggaatgcc ggaggcggga ggcagtttgt ctttagagtt tgcgattgaa
     2221 tatccatgcc agttgcgcgc gcgctatacg gaggaacacc tgcgcgcgtg ggggcggctc
     2281 ggcaacatcc atacgcgcgt gacgacgggc gagcaggccg cgccgtcgga agagaagctg
     2341 cgcttcgtcg agcgttcgag catcgagatc gagcggatgc aggaggagac gagcgtgtgc
     2401 gagaagtgcc ccgcgcgtct gccgcacgac atggcgggcg aaggcgaggc cgtcggctgc
     2461 caggggcgca tcacctaccc cttcgacgcg cagtttgaaa aatttctggc cgaccgtgtg
     2521 caactcgcgc tcgacaccgt gagcctcgaa gaccagccga ggctcctgcg catcctcgtc
     2581 gatcctgatt cgcctttcga cggcgaaggc gcgaagtcgc tgcgcgccgt gacgacgccc
     2641 gaaggcttgc gcttcttcga gttgcgcctg cccatccgcc tcgcacgcga agcctcgcac
     2701 gtcaccaccg atcatctctt cgacgcgctc gcgggcttca ccgccgaaga caacgcgcgc
     2761 acctcttacg cgcgcgaact gccccccaaa gccgcgcccg attattacga cttcctcgat
     2821 ctcattctcc tgaacgacct cagcggctcg gagcgcgccc gcatcgaaaa gcgcagccac
     2881 aactatcaac agtttctacg cctccacgcc gctctcgaac gcgccgaagc cctccaggct
     2941 cgcatcctcc tcgactaatt cccgacaccg gaattcgact caagagatca gcccgccagt
     3001 catcgctaga ccggcgggct gattttttcc tctcgcttta cttacctaat ttccacgcac
     3061 gagaaacacg tcccacgccc agcgtccgac ctgtgccccg cccgcgtctc tgactctggc
     3121 acgataacgg aagtggttgt cgtgttcgtc cgtccgtttc gactccttgt attcaaggtg
     3181 cagggcggcg acgttgagtg aggcgggcgt ctgtagttcc gccgcctcgg agaggccgtt
     3241 gtgattccgg tcttgccaca ggcgcaagtc cgcgaacacg ccatccccgc cgtcaatcac
     3301 gccgtcggca ttgcctccct tctccgggcg gtcgaagcgc gccagcgcga ggaagccgtt
     3361 gggcgtcgca gaccagagtt gcagcgtgaa gttgccgaac aactccccgc cgttgtctat
     3421 ctggccgttg ccgtttctgt cgagggcgag ccacgcatca tcggaatcga cggcagtcca
     3481 cgagagccat tctctgtgac cgtcgttgtt gaggtcgaag gccacaccat gcggcgcgtc
     3541 ggtgagattg aaaccatcgc ccgccacgtc tatcaagacg ggcgacgaac tgccgcagac
     3601 ggaactgttc cagcaaccgt ccagcgtgag gtcagccaca caagttcctt cggtttgcgc
     3661 gcccgtgcaa tcgccgtcac catcgccgcc gcccaacgga gcgtcagcag cagtaggcgg
     3721 aggaatctga gtacagttat ttgtggtatt agggtcggcg cttctattcg acttatagac
     3781 atcttcagct ttcacttcgc gggtaacttg tgcgttattt tcaccatgcc cgttcatgat
     3841 ggacggatta gttccgcacg gttcgcttga tgtattggca agtccgatgc gatgaccaac
     3901 ctcatgggca atagtggctt ctaaatcttc cgctgtgagt gagttcaaca catccgacct
     3961 gatgtgtatc ttatgcgggt aaacgtctaa atctatatga gcaactgatc ctaccgcggc
     4021 agccacaatt ataaaatctg ctgattcgta attgctctgt tgaaaaaagt aattggtcgt
     4081 ataactattt ggaggattgg aagttgtatc tcgcgcctga ttccacatcg ttgacgcacc
     4141 gtttatagcc tgaccgacct tagcattgtt ggctccggga acatcattgg taatgttcag
     4201 cacgcgccga gagtctgttg acgaggcggg gccatgacca tttagcggcg gctgattttt
     4261 gaaacatggc gggcatgccg attcaccgta agcagcaaga agtagcacta gaatgacggc
     4321 acatagcgaa actttataaa atcgcatgat tctgacagct tccttcaact aatcagcttt
     4381 tcggtatctg cgatactcaa tcaggaaaaa gacgttacct gctgcgcgca gttgcgaatc
     4441 tttctctaat ctccggtaaa gatttaccgt accgagcctt tagttctttt tcgagaggaa
     4501 gcgtcacgtc atttattggt gtgagcgagt tgtcatcgtt gatggttaaa acgcctgcgg
     4561 ggcctaaatc aggcaagccc actttaatgg agttgtctaa cagaacaaaa attaagtatt
     4621 ttctggattt tttgaacgta ggaaagatat gttcactacg cgtgacttta actccgtcca
     4681 ccgttaacgt cccgccgatt ctgggcacca agatttcatc atcattcaag ggcaacagtt
     4741 ctaccggcgg ttgaataaag tcaaaggatt tggctacggc aggttgagaa acaggctcta
     4801 aaattttgaa cttgtaccag gtggtgatgc tatcgtgttc gttcagatat gatctctctt
     4861 ctacaggctg cgcgataagc actgtgtatc gagtgagggc gtcatctaaa ttttttactt
     4921 gttcgtagtt tgcgataagg ccggacaaat taagctctag ctgcccattg cttttcgctt
     4981 tagcaatttg ctgtctaagc gtgccgcgtt tttctgattc gattggcact tgtcgaacct
     5041 tgtttttgga tgatgttttg atatatccga ttcccatgca ggctagaagg acaaatgaac
     5101 aaataatggt aatgcttttg taggacactt ctggctctcc ttaaatatca ctatgttgga
     5161 agtgggaggt aattaacgta atcagtgagg gttgctttaa ttagggcgta agcggcaaac
     5221 tgcttataca cccttcaatc ctacagatca acctcatacc aagacctcct cgatctcatc
     5281 cttgaacgac ctcggcggtt cggagcgcgc acgcaccgaa agtgtcagga ggcacgccgc
     5341 gagaggtgcg ccgacgtaca gcagttgcct gcgggattga ggatgtggct ccataaccca
     5401 actctgtcca tcataacttt tgcctctggc tcgtctctta gcacgctttt ccgggtcggc
     5461 aggctctccc gctgcgtagt ctgcaaccgg ctccggtgcc tctgccgcac tgtgctgtgt
     5521 gggcgcggcg gcgctcgttt gttcctggcc ggagagcgcg gttaatgccg acaaggccgt
     5581 gacggacagc acgaccagca gcagtaagat tttcctgtgt ctgtgcatat ttagactccc
     5641 ggttagcccc tcgacaggta aaggacatca ccactgatta ggaaagtttt cagttgccgt
     5701 gaggttgacg aaatcctctt agctttcgtt aacaggacta aagcgacaac aggcttatac
     5761 gcccgacgtg agaatacaga tcaagaaaag cacttaaaga gttgagttct gaatttggct
     5821 cttcccttta gccccttccc cgtttccctt atctattttc ctcatccaat tcgcggagcg
     5881 cgttcaagag gagactcgtg ttgctggtgg cttgaatggt ggtgggataa ctgccggttg
     5941 atctctcgaa atcgaattcg ccgccggtca tctcgaccag gcgacgcaac gcgtccgtgt
     6001 cgcgctcgac gccgctttcg gctgccgcga tgcgcccctc gttgaaacgg atgtggccgc
     6061 cttgtgcgcc gccgccggac agcgtgagcg tgcccgtcag ccgtgcgttt tcgatgacct
     6121 gcacggcgtc gaagatgctg acgaccgaga ggtcgcccgc gaaggtgagg gcgcgcggcg
     6181 cacgctgcgg cgcgttcgtc gcggcggaag gggcgggcgg caggtctggg cggcgtgcgc
     6241 ggcgaatggc ttcgaggccg ggcacgatgt tgatgcgcgg gtcggtctgt ttggcttcga
     6301 ggagccgttc ctgcgcggcg tcgaaattgt cgcgggtgat gtagaggttg gtgagcgcga
     6361 gacattgccg cgcggcctct tccggctggc cgttggcgag cgcgacctcg cgcagacgct
     6421 cgcgcagttt gatcgcctgc gggttgctct tgatcgcttc ctgtagacgc gccatcgcac
     6481 gcgtgtgcga cctgtatttc agaaacaact cgacatcgag cagagccgtc tcgacatcct
     6541 cgctgtaacc cgtgtccgtg tgcgcgtcct tcatcgcctc tcgtgcccgc aactgagaac
     6601 ttccagccgg gaaaagttta tcacactcgt cgcaacgcgc tgcgcgtgtg ctgcaagatg
     6661 ccgcacgggt gccggattta agtcgtccgc tgaccccttt tcgtatagtc cgccgccatc
     6721 ttttcgcagt tttttcgcgc tacgtctcac aacgtacata tctccgggca cgactcgtgt
     6781 atagtcacgc tcaggttagt tctcaaccta ctcttcccaa cttacagctt cgttatcctt
     6841 aacttttagc ggtttctgtc caacgcgttt tagccctgtg cagcggtaaa acgggcgttg
     6901 catagaggaa acatggcatg ccgtgtgcaa ttaaagctgc gctggtttct aacccccttt
     6961 aggttactca aactataatc gcgtgagagc gtccgccgga tgctcgacag tcgcccccca
     7021 ctgccgtcgc acggacagcg gcgcgtttag aggagtaaaa aaacggatgc aagaagagtt
     7081 gacgattttg gacgcgagcg cggcggcaac cgtccggctg cttaataata gctgggcgaa
     7141 ctatctccaa cgcgcgtgca cacgcctcaa gtcggtcgga caaattaatc aaaccaacgg
     7201 cacacacgcg ctcgtctcgc gtctcagacg cacgcgcccc tccacctatg agcactccgt
     7261 ccgcgtcgcg cgactcgcac gcgtcaccgg acgcgccttc aacttcacgg acacgcgcct
     7321 ccaacaactt tcttcgaccg ccatgctgca cgacatcggc aagatactcg tgcccgaagc
     7381 ggtcttgaat cgcccccgtc gcccgacgcg catggagcag ttcgtactgc acctgcatcc
     7441 gtcgctcggc gcgctcctcg cctcctattt caacctttcg ccggagctac gcatccgcac
     7501 gcaacatcac cacgagcgtt gggacggcaa gggctacccg caccgcctcg gcggcgaaga
     7561 catcccgctc atggcgcgca tcgtgcaagt cgttgacacc tacgacgcga tggtcgccag
     7621 acgcccctac tccaacccgc gcacacacaa cgaagccgtc gccgaactac tcgcacacgg
     7681 cggcaaacag ttcgacccgc gcgttatcga agcattcctc gacgcgcatc ccgcacatct
     7741 ggacgaaaca ggcttttagt taaaaaaatt tcgccagcgc aggaggccgc tttctctcgt
     7801 agaaagcggc ctcctgcgct tgagcgaccc gctcgacgct taactcaatt cttaaaaatc
     7861 gaatctcagc ccgactcgca cgctgcgcgg gcgcagtgtg tgcgtcggga cgaggaagga
     7921 gtcgaggaac gcctgacgtt gtgctgcggt cgccgtgggg cgcagggcgt tgaagttgat
     7981 gaactccgcg ccgaaggtgt agacggtttt gttgagcggg ttgtcgatct cgatggacgc
     8041 gcgcagggag agacgctcgc cgaagcggaa ctcgcgcgtg atgttgagat cgaaggtgaa
     8101 gagggcatcg ccgcgcccgg cgttgcgcgg gagatttccg ttgcgcccga tgatgggcag
     8161 cgagaacgcg cccggaagtt cgggattgag cggttcgccg gggcggcgcg agcgtatgtg
     8221 tcgcgggtcg ccgttgaaga caggccggtc attgctcacg tcgtcgagat tacgatccgc
     8281 gccgccgagc gaaaggttga aaggcgcgcc gctggcgacg cgtaagatcg tggcgaagcg
     8341 caaggctccg agtttgcgcg gcgtgtcgaa ggtgccggag agtgcgaagc gatggcgtcg
     8401 gtcgagcaga cccggcgcac gctcggcgcg aaagtctccg ggcgtgagtg cgctcgaagt
     8461 gttgactacg ccgtcgtcta tgagccgcga gagcgtgtag gcggcgcgca gggagaggcc
     8521 gaagccctgt tcgcggcggg cgaagcggcg gcgcgcttcg acggtcaggc cgtgatagaa
     8581 gctgttgccg attgatgtga gttgttcgat ctgcccgcgc gcggggtctg gacgcagatc
     8641 gttcaaggcc gcgagcgcgg cttcgagcgc gacgctcgaa ctgaaactgt cgagattgaa
     8701 gatgctgacg gggacgccga attctacgac gcgagcgatg gcgtccgggt tcggcgcgcc
     8761 gggcgcggcc agcgtgaagc gcacgagttc gccagccgac tgtgtgttgt agagcggtcg
     8821 tgtgccggac acgtcgcgga agttggcgaa gtcgcgtgag agcaggtagg cggcgaagtc
     8881 tctgtaaccg tgcgggagtc gcggcgcgtt ggcattgaac tcacgccaga gatgcagccc
     8941 gcgattgaag gtgtaattcg cttctacgac gaacccgcgc gcgagttcgc gctcgaagcc
     9001 gacgttggct tgatagcttt cggggatgcg caggtcggga tcgagtcggc gcgtgaagtt
     9061 tgactggagc gcgccgaatt gtttgacgat gggggaaccg gcgttgagcg tttcggggaa
     9121 acggaggttg gcggcgatga aggcgcgacg ctccgcatcc gtcataagtc tgccgctcgc
     9181 gggactgcgc agcgtgtccg tgtcgaagaa gatgcgcgcc cgaccgagcg tgaaatcgtc
     9241 aaccgtgcgc aggagcacgc ggttgtagaa cagacccgcg ccgagacgga tgacggtctt
     9301 gcccgtgcga aacggatcga gggcgagcgc gaggcgcggg gcgaagttgt ttcggtcttt
     9361 gaggatcgtt tcgttttcgt agcgcagccc gtaagagatc gtgaggttgg ggcgcgcgtg
     9421 ccattcgact tgcgcgaaga agcctgtgta gagattcttc tgctccgact cgccgttgaa
     9481 gcgttggcga aagcgtgagg gcgcgtcggc gagaaactcg cccgcgctct cgaaatcgaa
     9541 ggtgccgctt gcgtccgaga ggtctatgaa agttgaacgg atgccttgca ggtcgccgcc
     9601 gaatttgaac gtctgcgcgc cgcgaagaac ggttagagtg ttttgcagtt gcacgcgcag
     9661 ctcgcgccgg tcgcttgcac ccgcgttcga cgaaccggcg acgagcgtgc ccgaacgatc
     9721 tgccgtcgag acggcggcga gcggatcgtc aatcgagatg agcacgacgg gacgcgcttg
     9781 cgagtccgct gttcgcaacg ccggggtgag ccttgaaatt tgcgcacgga gttgattgac
     9841 gaggcggggc gagccgacga agttgtccgt gtaggcgagg gcatcggaat ggcgcgtgcg
     9901 gccttgcagc gcatcggcga ggcgcgtgcc gccgccgaac tgtcgcaggt ttcggctgcg
     9961 cccgcgttga tagagaaacg tggcgttgtg cgcgtcggag aagttgtgat cggtgcgcgc
    10021 cgtgaagttg taggcgcgca ggggcgtagc cgtgtgctcg acgtagggcg cgagttcggc
    10081 gggttcgaac ggcgcggcgg atgaggcttc cgtgcgccgc ccggcgagcg tggtgggcgc
    10141 gggcagaggg aagcgcgggt ttgcgcgcac gggaacgagc gtgtcgatga cggtcgcatc
    10201 gagaactatt tgagattcgt gagcaatgaa aaaatggtgt cgcgacgggc gagttgcgcg
    10261 gttcccgtct gcacctccgc tctctgttgc gatggcgcgc acgccatccg gtgctcccgc
    10321 tctgtcgcgc gcgccgaaaa gtttcagggg cgacttgagt tggccggaga gagtgaagcc
    10381 gggagcgtgc tcttgcaggg gcagacgctt gaggccgcgc gcgttgttga aatagctatt
    10441 ggcgttcaac gattcgtcgc gaaaaaaata gaaggcgcgg gcgtgaaaat cgtgcgcgcc
    10501 gccgcgcgtg cgcaggttca ccctgccgcc ggatgcgcgc ccgtactcgg cggagaactg
    10561 gttggtgatg acctgcactt ctgcgacggc ttcgagcgat ggttgaaaac gttcgcgcgc
    10621 ggcgcggtcg tcgttgttgt cgagtccgtc aatcgtgatg ttgttggaat aggccgcgcc
    10681 gcctgagagc gcgaacgtgc ccgcttcttc cggcgtgcgc gcgggcgtgc gattgcgatc
    10741 ctcggccacg tcgcgcgtcg agagcggctc ttccgacacg ccgccgagcg tgtagatgag
    10801 atcgagcggc gagcgcgtag gcgacggcaa cgtctccaac tcggcgcgcg tgagcgtgcc
    10861 gccgaccacc gtgcgcgtcg tgtcaaccac gttgccactc gcctcgctta agacgacttg
    10921 ctcggcggcg acgcccgccg gtcgcagggt gagatcaagt tgcacgcgct gtcccgctaa
    10981 agtctcgacg cccgcgcgtt cctgcgtggc aaacccttca caggctgcgc gcaacgtgta
    11041 gacgcccggc gcaagttcga ccagacgata acgcccctcg ctatccgcga cgaccgagcg
    11101 cgcgaagttt gtggcggcgt gaacgactga cactttcgcg cccgcgatgc ccgccccgtt
    11161 ttggtctatg acgcgcccgc tgatcaccac gtcgtcgagg tcttgcgcga acgctgcgcc
    11221 gacgccgtag tgagccgggg cgagaggaga caaaaggagc gcaaacaggc aggggcaaat
    11281 ttgccagagg cagacgtgag agatggtgtg cagggtgcgg ggtcgcatgg gctgtctcct
    11341 gtcgcgcggc ggctgacagg tgaggtcgcc gccgcaggtt gtgtcgcctc caccgcgtgg
    11401 cgacgaaggc a
//
LOCUS       DASWNS010000050         6523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_27575, whole genome shotgun sequence.
ACCESSION   DASWNS010000050 DASWNS010000000
VERSION     DASWNS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27575"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VGB76_01745"
     CDS             complement(<1..697)
                     /locus_tag="VGB76_01745"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY0097651.1"
                     /translation="MTGKTDLAYQALLRSSAIRVNRVWVFFRLLRLLYFGFYFWVGLL
                     WHRLTITNVEERQKIDAKRFRRLLVNRGGVMIKVGQQLSQRPDVLPPVYCDELRTLLD
                     DLPIKIDKKDVILAIRRQTGMAVDEAFADFNFDPVGSASVSCVYRAVLHEGDVVAVKI
                     RRPNILKEFTADLDAINWLFFFLEFLTVWRPGTTKHIRYELRDLLLEELDFRREARYQ
                     ELFRRYHRRRKKLK"
     gene            complement(1022..1330)
                     /locus_tag="VGB76_01750"
     CDS             complement(1022..1330)
                     /locus_tag="VGB76_01750"
                     /inference="COORDINATES: protein motif:HMM:NF039376.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097652.1"
                     /translation="MDKDNNQTSVAVVVQDIPTPVIVDLGSRKKKAIKRLKNGRGKLM
                     SEVALAVEQVRGGLSEGDQNQRIVPVVIIYKKKKKNKGGGKGMSLSPLNALSPFNFLR
                     "
     gene            2351..2860
                     /locus_tag="VGB76_01755"
     CDS             2351..2860
                     /locus_tag="VGB76_01755"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY0097653.1"
                     /translation="MEVSDGATAGAHLIYLRRAVWLALEAEAAGNLPVGAVITLDDGV
                     IAEAGNSILRPHYYPGGHAEAESLRRVPARLWSRAREMTCYTTLEPCVMCMGAILLHG
                     IGRVVFGSSDREGGAGQLLPHLPAYYARGAPVPEWIGPLLPELCDALYERAREKFDEL
                     PCGKSFRGE"
     gene            complement(2993..3133)
                     /locus_tag="VGB76_01760"
     CDS             complement(2993..3133)
                     /locus_tag="VGB76_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097654.1"
                     /translation="MNVQTRGLTCGIVFDDEIPPPAPGEPDETDEDEPVDEEEEDDVE
                     FA"
     gene            complement(3166..3777)
                     /locus_tag="VGB76_01765"
     CDS             complement(3166..3777)
                     /locus_tag="VGB76_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097655.1"
                     /translation="MAKTQGTARKTAPGASLATTAGDDELTKDALQRSMEDARESIAQ
                     TVTDIKDTVAGQYQSVKESVTDALDWREQFRQHPVAWCVGALSVGFVLSDSVVAALKK
                     TDRENQLLDQLAALADHFSQQLTDKGMSILAPALTGTVLVPILTSKINEMFGVDLSHL
                     PQQLLPSDDTGVNKRAKGKKKGGKKKKKKEKKDKAGRRDENAA"
     gene            complement(3802..4089)
                     /locus_tag="VGB76_01770"
     CDS             complement(3802..4089)
                     /locus_tag="VGB76_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097656.1"
                     /translation="MPVIKRKTKKAITKQVKKLVKKHGPEIALGLATTLVGSVVGAAT
                     GKSQDKSKSKKKGDSAKKKEKPQKKSKREDAKTEETAKNKKKQTTAAATKS"
     gene            complement(4289..6448)
                     /gene="ppk1"
                     /locus_tag="VGB76_01775"
     CDS             complement(4289..6448)
                     /gene="ppk1"
                     /locus_tag="VGB76_01775"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY0097657.1"
                     /translation="MEDKKQQLPHVGQFENASGVAITDDTSYSTLHLLNRDLSQLEFF
                     RRVLEEARDPSQPLLERLKFLSIFSTNLDEFFMIRVSGLKERAEHKGIEATPDGLMPA
                     DQLSLIRERLLPMFEEQARCLREEILPALAEQGIAVVAYASLSRTEQAALKLFFMDSV
                     FPLLSPQSVDQAHPFPYIPGLNLNLGLMVGPRPEHGITRSLVGGIKPRFVRIAVPALV
                     PRLVPVAEGQTRYVYLEELIAANIGELFPRMLAGEVYPFRVTRDADFDVREDEVVDLL
                     QTVEQSLRTRRFGSPVRLEVASDMPPEMTRFLRDSLDGLTAEDVYVTDAPLNLPDLMT
                     LYSLDKPELKDRPLQAKNRVKLTGRETIFDIIGSRDVLLHHPYDSYSTITDFIDAAAH
                     DPNVVAIKMCLYRTGKNSPIPQSLIKAVKNGKEVVTIVELKARFDEEHNIEWAKRLEE
                     AGAHVVYGLIGLKTHSKVALVIRREDERLRSYVHVATGNYNPTTSRFYTDLSLLTADR
                     EIGSDASALFNFLTGFSRQKEYRRLLVAPVNLRGRMLELIRRETAHARAGKEARITAK
                     INRLADLEVIESLYEASQAGVTVDLIVRGICTLRPGVQGLSERITVRSIVGRFLEHSR
                     VFYFANGGAEELYIGSADWLPRNLNRRVEVVTPISDPDAKTYLKDVLLKAYLRDNVRA
                     RLLRPDGSYERVQTTPDEARWESQLYFEGVGDATKEV"
BASE COUNT         1262 a         1895 c         1874 g         1492 t
ORIGIN      
        1 ctttcaactt cttacgtcgt ctatggtaac gacggaatag ttcctgatag cgggcttccc
       61 tgcgaaaatc gagttcttcg agcagtaaat ctctcagttc gtacctgatg tgcttggtcg
      121 ttcccggtcg ccatacggtc aggaactcca gaaaaaagaa gagccagttg atcgcgtcga
      181 ggtctgccgt aaattccttc agaatattcg ggcggcgaat tttgacggca actacgtcgc
      241 cttcgtggag tactgcccgg tagacgcagg agacggaagc cgagccgacc gggtcgaagt
      301 tgaaatcggc aaacgcctcg tccacagcca ttcccgtctg gcgacggatg gcgaggatca
      361 cgtccttctt gtcaatctta atcggcaggt cgtccagcag cgttctcaac tcgtcacaat
      421 agacaggcgg cagaacgtcc ggtcgctggg agagttgctg tccgactttg atcattacgc
      481 cgccgcgatt cacgagcagg cggcggaagc gtttggcgtc aatcttctgg cgttcttcga
      541 cgttcgtgat cgtcagacga tgccagagca ggccgaccca gaagtaaaac ccgaagtaca
      601 agagccggag gagacgaaaa aagacccaga cgcgatttac cctgatcgcg ctcgaacgca
      661 atagagcctg ataggcgagg tctgttttac cggtcaaaaa atccggggaa tggagcgggt
      721 cacgctcgga tgcgttcgtg ttccgccaat tctctagctc cggcttctca tgggttacgc
      781 cccatcctct cgggtcgcct ccctccgcaa ccaaaccgtg tccattccct tgataagatc
      841 gagccattac agaagcaacc tcgaatcctt tgcgctcgcg ccctagacca cagcactttt
      901 agctgtcgcc cggtcgtctc ccgacaacat caatccgctt ccagagagcc gcgcgagccg
      961 cgctgtcaaa agcggctttg tgtgcgcgac aagcttctct ccgggcggca acttctcgcc
     1021 tttaccgcag gaagttgaaa ggagaaagag cgttgagcgg agagagcgac atgcctttac
     1081 cgccgccctt gttcttcttc tttttcttat agatgatgac caccgggacg atccgctggt
     1141 tctggtcgcc ctcggacaaa ccgccgcgca cctgctcgac ggctaacgcc acttcgctca
     1201 tcaattttcc ccggccattc ttgagtctct taatggcctt ttttttcctg ctgcccagat
     1261 cgacgatcac cggcgtcgga atgtcttgaa ctaccacggc gacgcttgtt tgattgttgt
     1321 ctttgtccat cgtgtgcctc cgaatcttgt tgattcctat tacccgagta ctgttagttg
     1381 actatcttct tccgcttgcg cccctcgttt gaaaaccggc ttacacgccg ccgggagcgc
     1441 agagagagat gtgagcagaa ctgaaagtcc cacggcttca aagatgaatg ccctcgcccg
     1501 tttgtgccgg gagtcgaggg gataaagaag gtgcaccggc aaccgcagag atatgttgtt
     1561 gcatatgctc caggccgcgt gcgttagctc ccggaaagcg tcgagccgat accgcgtaaa
     1621 gcaataatgc tccggactac cgttgcggca tccgcgaggt gcgccccatg ttgtaaccag
     1681 cttgcttaat gccctcgacg aatccccgat ctgctttgcg ggcacggcct ttgcttacca
     1741 acgccttaga ggaacaaaac attctcaggg tcgagattgt gaatgagcag gtcacggcgg
     1801 cacgcctgtc atatcgccgt gaagcacctg tcggcttttc cggtcgcccg gatcaggtgc
     1861 gccgttaacc ggaagctccg tttgacggct gtatccgttc tctttcctgc cgcatcgctt
     1921 ccgactcgac aaaaaatttg cggaacgaat gaaagtcttg tttgaaagag cctgagcttg
     1981 agtaactaat ggtgacggta aagctaacct ctgagggtcg gcttttggtc ttggacgttg
     2041 agcctctcaa catcctgatg cgctagaatt cattctgagg catggtcgcc gcgctgattt
     2101 tcacggctga ccacgagcga tgtgtccggc acttcaacaa aatccttgga acctttcgca
     2161 ggcagctata atggcaatcg gtggaaaaat gagtttgcga aagcgcgccc gggattaaag
     2221 gaaaggcgca tatagcttgc gtgctcgtag cggtaaagca aaagaacatg agccttatac
     2281 tcccttgcaa gaaaagtgtc aagaaaatcg ttcggctccg tcacgtcact gtaggaagca
     2341 ggaatctcta atggaagttt cagacggcgc gactgccggg gcgcatttaa tttatttgcg
     2401 gcgcgccgtg tggctggctc tagaggcaga ggcggcgggt aatttgccgg tgggagcagt
     2461 tattacgctg gacgatggtg tgattgccga agcgggcaat tcgattttac gaccccatta
     2521 ttatcccggc gggcacgcgg aagccgagtc gctccggcgt gtccccgcgc gcctgtggag
     2581 ccgtgcgcgc gagatgacct gctacacgac gctcgaaccg tgtgtgatgt gcatgggagc
     2641 aattctgctg cacggcatcg ggcgcgtagt cttcggttct agcgaccggg aaggcggggc
     2701 gggtcaactc ttgcctcacc tgcccgcata ctacgcacgc ggcgcgcccg ttcccgaatg
     2761 gattggtccc ctgctgcccg aactctgcga tgccctgtac gagcgagcca gagagaaatt
     2821 cgatgaactg ccgtgtggca agagctttcg cggtgagtaa cgaaagcggg tcgggaggtt
     2881 tcaaagtttc gttgacaaga ggcgcggagt cgcttgggga tgagccgcac agacgcttga
     2941 actcatcgct tgctccggtc aacacgatgc agacgcgaag ttcacttcgc ctctaagcga
     3001 actccacatc gtcttcctct tcttcatcaa cgggttcgtc ttcatccgtc tcgtccggct
     3061 cgccgggagc gggcggaggt atctcgtcgt cgaacactat cccgcaggtc agcccacgcg
     3121 tctgcacgtt catctacaac gtcctttctt tcagagcggc caccctcaag ccgcattttc
     3181 atcacgccgc ccggctttgt ccttcttctc tttcttcttc ttctttttgc cgcccttctt
     3241 cttccctttc gcgcgtttat tgactcccgt gtcgtcgctt ggcagaagtt gctgcgggag
     3301 atgcgagaga tcgacgccga acatctcgtt gattttgctc gtcagtatcg gcaccagcac
     3361 ggtgcccgtc agggcgggcg cgaggatgct cattccttta tcggtcaact gctggctgaa
     3421 atggtctgcg agcgcggcca gttggtcgag caactgattc tcgcggtcgg tcttcttcaa
     3481 agcagcgacg acgctatcac tcaggacgaa gcccaccgag agcgcgccga cgcaccatgc
     3541 gaccgggtgc tgccgaaact gctcgcgcca atcgagcgca tccgtaaccg actctttaac
     3601 cgattgatac tgacccgcca ccgtatcctt gatgtccgtc acggtctgcg cgatagactc
     3661 gcgcgcgtcc tccatgctgc gctggagcgc gtctttcgtt aactcgtcgt cgcccgccgt
     3721 cgtcgccaac gacgcgccgg gtgcagtctt gcgcgcggtt ccctgcgtct ttgccatcgt
     3781 tccctccttg attaaacagc ttcagctctt agtcgccgca gcggttgtct gcttcttctt
     3841 attcttcgcc gtctcttcgg tcttggcgtc ctcgcgcttc gacttcttct gtggcttctc
     3901 ttttttcttc gccgaatcgc ccttcttttt cgacttcgac ttgtcctgag atttgcccgt
     3961 ggccgccccg acgacgctgc cgacgagagt ggtcgccaat cccagagcga tctccgggcc
     4021 atgttttttg accagcttct tgacctgctt ggtgatggcc ttcttggtct tgcgtttaat
     4081 taccggcatc cgatcctcct ccgcaatatc agcctgcgcc agctcatcga acctcttcgc
     4141 ggctcacgcc agagacgcga ctttcaaagt tctgagccgt caggcaaact atagcaagtt
     4201 accgccgcga ctgccgctgt tcctgatgaa aacagcccct gcaaaagttg aaaaatcggg
     4261 cggggatgaa ctccgtctga aatccacctt aaacttcttt cgtcgcatcg ccgacgcctt
     4321 caaaatatag ctggctctcc caacgcgcct cgtccggagt tgtttgcacg cgctcgtaag
     4381 agccatcggg acggagcagg cgcgccctca cgttgtcgcg caggtaggct ttcaggagaa
     4441 cgtctttcaa gtacgtcttg gcatccggat cgctgatcgg cgtcaccact tcgacgcgcc
     4501 ggttcagatt gcgcggcaac cagtccgcac tgccgatgta gagttcttcc gcgccaccgt
     4561 tggcgaaata gaagacgcgg ctgtgttcga gaaagcgacc gacgatgctc cgcacagtga
     4621 tcctctctga caacccctgc acgccggggc gcagcgtgca gatgccgcgc acgatgaggt
     4681 caacggtcac gcccgcctgc gacgcttcgt agagcgattc gataacctca aggtctgcaa
     4741 ggcggttaat cttcgccgtg atgcgcgcct cttttccggc gcgtgcgtgc gccgtttcgc
     4801 ggcggatgag ttcgagcatg cgcccgcgca gattaaccgg ggcgacgagc aggcggcgat
     4861 attccttctg gcgcgagaag cctgtgagga agttgaagag cgcgctcgcg tcggagccga
     4921 tctccctgtc ggcggtgagc aggctgaggt ctgtgtagaa tcttgaagtt gtcgggttgt
     4981 aatttcccgt ggcgacgtga acgtaagagc gcagcctttc gtcttcgcgc cggatgacga
     5041 gcgcgacttt ggaatgcgtc ttcaacccga tcaacccgta gacgacgtgc gcgcccgcct
     5101 cttcgagtcg cttcgcccac tcgatgttgt gctcctcgtc aaagcgcgcc ttgagttcga
     5161 caatggtgac cacctctttt ccgttcttga ccgccttgat cagggattgc gggatcggcg
     5221 agtttttccc cgtgcgatag aggcacatct tgatcgccac gacgttcggg tcgtgcgccg
     5281 ccgcgtcgat aaagtccgtg atggtgctgt aagaatcgta aggatggtga aggagaacgt
     5341 cgcggctgcc gatgatgtcg aagatcgtct cgcgccccgt gagcttcacg cggttcttcg
     5401 cttgcagcgg cctgtctttg agttcgggct tgtcgagact gtagagcgtc atcaggtcgg
     5461 gcaggttgag cggcgcgtct gtgacgtaca cgtcttccgc cgtcaggccg tcgagggaat
     5521 ctctcagaaa gcgtgtcatc tccgggggca tgtcggaagc gacttcgagc cggacgggcg
     5581 aaccgaaacg gcgcgtgcgc agagattgtt cgaccgtctg caagagatca accacctcgt
     5641 cttctctcac gtcgaaatcc gcgtcgcgcg tcacacgaaa cgggtagact tcgcctgcga
     5701 gcatgcgcgg aaacagttcg ccgatgttgg ccgcgatcaa ctcttcgaga tagacgtagc
     5761 gcgtctggcc ttcggcaacc ggcacgaggc gcggcacgag cgcgggaacg gcgatgcgca
     5821 cgaagcgcgg cttgatcccg ccgacgagcg atctggtgat gccgtgttca ggtcgaggtc
     5881 cgaccatcag gccgaggttg agattgaggc cggggatgta ggggaacggg tgcgcctgat
     5941 caaccgactg cggcgagagc aaggggaaca cactgtccat gaagaagagt ttgagcgcag
     6001 cctgctccgt ccgcgacagc gaggcgtagg cgacgacggc gatgccctgt tcggcgagcg
     6061 cggggagaat ctcttcgcgc aggcagcgcg cctgctcttc gaacatgggg agcaggcgct
     6121 cgcgtatgag gctgagttgg tctgcgggca tcagcccgtc gggcgtcgcc tcgatgccct
     6181 tgtgctccgc tcgctccttc aacccggaga cgcggatcat gaagaactcg tcgagattgg
     6241 tggagaagat ggagagaaac ttgaggcgtt cgagtagagg ctgcgaaggg tcgcgcgctt
     6301 cttcgagtac gcggcgaaag aattcaagct ggctcaagtc gcggttgagc aggtggagcg
     6361 tggaataaga ggtgtcatcg gtgatcgcca cgcccgaagc attctcgaat tggccgacgt
     6421 gcggcaattg ctgtttctta tcttccatca agttgtttcc gtgattacaa aatatatttt
     6481 gctttggaga gtctggcaat aatacgacac acgcgccctg cgt
//
LOCUS       DASWNS010000051         3311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_157413, whole genome shotgun sequence.
ACCESSION   DASWNS010000051 DASWNS010000000
VERSION     DASWNS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157413"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VGB76_01780"
     CDS             <1..519
                     /locus_tag="VGB76_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0097658.1"
                     /translation="RVRGMFAEGALKAEHPVSGVSWYEAEAYARFMGKRLPTEAEWEK
                     AASWDASSNRKRLYAWGDDEPSHAHGNFDLRYWDTTPVGSFRAGASGYGCLDMTGNVW
                     EWTSDLFAGYENFKAYPYPEYSEVWFDEDHRVLKGGSWATHPSVLRNSFRNFFRRHFR
                     IAFTGIRLASDA"
     gene            complement(516..959)
                     /gene="mscL"
                     /locus_tag="VGB76_01785"
     CDS             complement(516..959)
                     /gene="mscL"
                     /locus_tag="VGB76_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY0097659.1"
                     /translation="MFREFTEFIKRGNVVDLAVAVIMATAFGKIVTTLVDSIIMPPIG
                     LLLGGVDFTSLFINLSDKPRPASLAAAKAEGIPVIAYGQLLNDLITFLIVAFVVFLIV
                     RAVNRLKSRQATDAAPTTKDCPHCLSTIPLKAARCASCCVDLQVV"
     gene            1174..1440
                     /locus_tag="VGB76_01790"
     CDS             1174..1440
                     /locus_tag="VGB76_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097660.1"
                     /translation="MSELREQRWSVVSERGCEASGVNYEAAAALVRRLREDKVHGLCI
                     VSDEAARRYNANNNGATGAKTTEPEPAAPPAKKRAPRRKSAKLS"
     gene            complement(1485..2306)
                     /locus_tag="VGB76_01795"
     CDS             complement(1485..2306)
                     /locus_tag="VGB76_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0097661.1"
                     /translation="MRGQLTLLSSNLRPPASDLYSMRIVTYNVHKCRGLDRRVRPARI
                     ASVLREVDADIIALQEVLSVGGAAREMDQARFIAEELGYDYCIGENRRLSGGAYGNVI
                     LSRLPWRHVHNYDITWRGRERRGCLRVDIEMGDDARSDGNGNGTTRGALLHLFNVHLG
                     TAYIERRHQARQLVGDKILGNAELTGARIVLGDFNEWTKGLATRLLTEQLQSADVRTH
                     LRSPRTYPGALPLVHLDHIYFDPALELEQLTLHKSLTALVASDHLPLVADFRVRA"
     gene            complement(2329..>3311)
                     /locus_tag="VGB76_01800"
     CDS             complement(2329..>3311)
                     /locus_tag="VGB76_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY0097662.1"
                     /translation="VGDEFVKVLTERARAGVQVNLVLDAIGSFASWNSYFKELRAAGG
                     RVCWYHPFKWYTLPRINNRTHRELIIVDGLVGFLGGAGFADHWRYPVKDHPRWRDSMF
                     RVEGDAVANLQATFAENWLEASGEILAHKDYFPACRAEECTPSLVVNSSPSTGRSTRA
                     RILFQMLLASAQESVLITTPYFLPDRSARAEMVRAIKERGVKIQIIVPGKKSDHALTR
                     NSSRRLYGDLLAAGAKIYEYNPTMIHTKTLVVDGMWSVVGSTNFDNRSFGINDEVNLA
                     AFDTNLAARITEDFERDRAESKAVSYEEWKRRSVFERIHELFGMLLERQQ"
BASE COUNT          613 a          979 c         1094 g          625 t
ORIGIN      
        1 cgcgtgcgcg ggatgttcgc ggagggcgcg ctcaaggcgg agcatccggt gtcgggcgtg
       61 agttggtacg aggcggaagc ctatgcgcgc ttcatgggca agcgtctgcc gacggaggcc
      121 gagtgggaga aggccgcgtc gtgggatgcg agttcaaacc gcaaacgtct ctatgcgtgg
      181 ggcgacgacg agccttcaca cgcgcacggc aatttcgacc tgcgctactg ggatacaact
      241 ccggtcggaa gcttccgcgc gggcgcgagc ggctacggtt gtctcgacat gacgggcaac
      301 gtctgggaat ggacgagcga tctctttgcc ggatacgaaa acttcaaagc ttatccttat
      361 cccgaatact ccgaagtatg gtttgacgaa gaccatcgcg tcctcaaagg cggctcatgg
      421 gcgacgcacc cgtccgtcct gcgcaactcc ttccgcaact tcttccgacg ccactttcgc
      481 atcgccttca ccggaatccg tcttgcttca gacgcctaaa ccacctgcaa gtcaacgcag
      541 cacgaggcgc agcgcgcggc cttgagcggg atggtcgaga gacagtgcgg gcagtctttg
      601 gtcgtcgggg cggcgtcggt cgcctgtctg ctcttgaggc ggttgacggc gcgcacgatg
      661 agaaagacga cgaaggccac gatcaggaag gtgatgagat cgttcagcag ttggccgtag
      721 gcgatgacgg ggatgccttc ggctttggcg gcggcgaggc tcgccggacg cggtttgtcc
      781 gagaggttga tgaagaggct ggtgaaatcc acgcctccga gcagcaaccc gatgggcggc
      841 atgatgatgc tgtcaacgag cgtcgtgacg atcttgccga aggcggttgc catgatcacc
      901 gcgacggcga ggtctacgac gttgccgcgt ttgataaact ctgtgaactc tctgaacatt
      961 ttggtcgctc cgaagttttc ccgaagggtg cgcgagggcg gacgggagag ttaggtcgag
     1021 cggcgaggtt tctattgtat ttacacggca aagtcaaacg gaagcaactg ccctgcggcg
     1081 cggcggcggc ggcctgtctg gcgttccgcg ccgccttcca ttaaactcgc gtgcagcttc
     1141 gatttcagtt aactttcgtc ggcaagaata ttgatgagcg aacttcgaga acaacgctgg
     1201 tcggtcgtga gcgagcgcgg ctgtgaagcg tcgggcgtga actacgaagc cgccgccgcg
     1261 ctggtgcgcc gtctgcgcga agacaaggtt cacggcctct gcatcgtcag cgacgaagcc
     1321 gcgcgccgct acaacgccaa caacaacggc gcgaccggag ccaagacaac ggagccggaa
     1381 cctgccgcgc cgcccgccaa aaaacgcgcc ccgcgccgca agagcgcgaa actctcttaa
     1441 gcgcaattca cacgccgcat aactagaacg cccggcagct ttcgctacgc gcgcacgcgg
     1501 aagtccgcga cgagcggcag gtgatcggag gcgacgagcg cggtgagact tttgtgcagc
     1561 gtgagttgtt cgagttcgag cgcggggtcg aagtagatgt gatcgagatg gacgagcggg
     1621 agcgcgccgg gataggtgcg cgggctgcgc aggtgcgtgc gcacgtctgc gctctgtagt
     1681 tgctcggtca ggaggcgtgt cgccaagccc ttcgtccatt cgttgaagtc gccgaggacg
     1741 atgcgtgcgc ccgtcagttc cgcgttgccg agtatcttgt cgccgacgag ttggcgcgcc
     1801 tgatgccgac gctcgatgta agccgtgccg agatgcacgt tgaagagatg gagcagcgcg
     1861 ccccgcgtcg tgccattgcc gttgccgtcg ctcctggcat cgtcgcccat ctcgatgtcc
     1921 acgcgcaggc agccgcgacg ctcgcgcccg cgccaggtga tgtcatagtt gtgtacgtgt
     1981 cgccagggca ggcggctgag gatgacgttg ccgtatgcgc cgccggagag cctgcggttt
     2041 tcgccgatgc agtaatcgta accgagttct tcggcgatga agcgcgcctg gtccatctcg
     2101 cgcgccgcgc cgcccacgct caatacctct tgcagcgcga tgatgtcggc atccacttcg
     2161 cgcaacaccg acgcgatgcg cgccggacgc acgcgccggt caagcccgcg acacttgtgc
     2221 acgttgtacg taacgatacg catggagtag aggtcggaag ccggaggccg gaggttagaa
     2281 gagagcagtg ttaactggcc tctcaccttg tggcctccga cctccgaatc attgttgtct
     2341 ctctaagagc atcccgaaca gttcgtggat gcgttcgaag acggagcgac gtttccattc
     2401 ttcgtaggag acggccttgc tctcggcgcg gtcgcgctcg aagtcttccg tgatgcgcgc
     2461 ggcaagattc gtgtcgaagg cggcgaggtt gacttcgtcg ttgatgccga acgagcggtt
     2521 gtcgaagttg gtcgagccga cgacgctcca cataccgtca accacgagcg tcttggtatg
     2581 gatcatcgtc gggttgtatt cgtaaatctt cgcgcccgcc gcgagcaggt cgccgtagag
     2641 acggcggctg gagttgcgcg tcagggcatg gtcgctcttc ttgccgggca cgatgatctg
     2701 aattttgacg ccgcgctcct tgatggcgcg caccatctct gcgcgcgcgc tgcggtccgg
     2761 gagaaagtag ggcgtcgtga tgaggacgct ctcttgcgcg gaggccagaa gcatctgaaa
     2821 aaggatgcgc gcacgcgtcg agcgtccggt cgagggcgaa ctgttgacga cgagcgaagg
     2881 cgtgcattcc tccgcacggc aggcgggaaa gtagtccttg tgcgccagaa tctcgcccga
     2941 cgcttcgagc cagttctccg caaacgtcgc ctgtagattc gccaccgcgt cgccttccac
     3001 gcgaaacatc gagtcgcgcc agcgcgggtg gtctttgacc ggataacgcc agtggtctgc
     3061 gaagcccgcg ccgccgagga agccgacgag tccgtctacg atgatcagtt cgcggtgcgt
     3121 gcggttgttg atgcgcggca gcgtgtacca tttgaaaggg tgataccagc agacgcgccc
     3181 gcccgccgcg cgcagttctt tgaagtagct gttccacgaa gcgaaactgc cgatggcgtc
     3241 gagcacgaga tttacctgca cccccgcgcg cgcacgctcc gtcaagactt tgacgaactc
     3301 gtcgccgacg c
//
LOCUS       DASWNS010000052         6908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_391047, whole genome shotgun sequence.
ACCESSION   DASWNS010000052 DASWNS010000000
VERSION     DASWNS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391047"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VGB76_01805"
     CDS             complement(<1..321)
                     /locus_tag="VGB76_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097663.1"
                     /translation="MRVIKTKGDFRAKAIAGTHTVLIALDCAEERRQGLMGFAFRRDV
                     VSGENMGPKWLRSLKVFKSVVPDPRALNAEGKEIRFSTWEHPIQSFLWSDYTAKPDTV
                     YKFTV"
     gene            complement(355..1473)
                     /locus_tag="VGB76_01810"
     CDS             complement(355..1473)
                     /locus_tag="VGB76_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEY0097664.1"
                     /translation="MPIRNLLPHGGRFVALLMCLGVTAMLPGTSLAWGDEGHRITARI
                     AASFLTPAAAAEVGKLLRNDARNNPAYFKSKCPAMFALTSKAKLTKAEEATFLVEGLA
                     CVAPWPDPPVKDQRPYTANWHFVDIPVIMQNPDGPQRFTYEPARDCVMDSKRGDCAIQ
                     ALERLQAVLRNQKVPNEKGHEWGEELTSRSEALKFFVHILGDMHQPLHNVADKADVKA
                     VNDPKNMGDVGGNRKYAEWFGDDKTPYGPMNLHSVWDSGFITHTMAVGNLSEDQYYRR
                     LLQELKAGGSSGLAQMQAGDVFAWTGESYNTAVQRVYGKLPPLNLGCGVKDVKSGKLS
                     GCYRLADEYYSANKSVVDQQLKLGGVRLAKLLNEALGN"
     gene            1757..1978
                     /locus_tag="VGB76_01815"
     CDS             1757..1978
                     /locus_tag="VGB76_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0097665.1"
                     /translation="MNAKLREHVRRVMQERGLTVYRVAQGSGERLSTDRLDDIRQARA
                     ENLSDSEMEGLAKGLGESADYLRSLIDTP"
     gene            2253..2774
                     /locus_tag="VGB76_01820"
     CDS             2253..2774
                     /locus_tag="VGB76_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097666.1"
                     /translation="MIGGLVCNDVVEPGLLPGDGQELSHRTREFLWRIFVEIGNCWKN
                     VWWESSNLKSSWLDLVKAKSECPPSYNGEYVNAVYVVQELLDMYGEPYAFYKLFFESG
                     IPSGPPTTRLAHAKRYVVDEFIRMQVLASGFKHFGGKNSNYKGYVKGSRYSLMPEVRE
                     FIESAEESKAESE"
     gene            2787..4346
                     /locus_tag="VGB76_01825"
     CDS             2787..4346
                     /locus_tag="VGB76_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY0097667.1"
                     /translation="MKTKYILVGSGVAGTTIAKRLLEHDRNTSILMLEAGPVIEAKSR
                     RGWWDHVTLPGERTPYNYTYDLDWEAKTTGNVNFISAGARVKAYGGSTMHWGAWALRF
                     KPEDFHLYSNTGEGGKWPITYDDLEEYYCQAEEYMSVCGDDGEKWSRRSKPYPMPPFK
                     WTAADGLMIKAFREVGIKPGKMPIARYRKCMTTGTCKYCPFGARFSAQYVLDDLREDP
                     RHQNFIVRPNSPATKIVCSSRDQIEAIEYVDNQTGETIREHADTYIICSGAYETPKLL
                     MNSTNHFWENGVGNDHDLLGRFIISHSMLKVRGSSPTNKEKWFQEYDFPTLMSRTYDT
                     EEQQKKGKIFLFKNRVLPNIDIASLMIGGNTPAQIDKIVSGEMELELQAFMEEKGKFE
                     NRLQIIPGKDRFGLPLTTVNFNRTDEERKNTEDRLSIMRKVIDAMGLKFVFGKTEAPG
                     AHHTTGTCKMGNTPEEGVTDSNLKVHDIKNLYLCSNGVFPTGSAVNPTLTLAALSMRL
                     TDHLISQSNGN"
     gene            4353..5453
                     /locus_tag="VGB76_01830"
     CDS             4353..5453
                     /locus_tag="VGB76_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like protein"
                     /protein_id="HEY0097668.1"
                     /translation="MSVAQQQIEKKVEIVLSLENPPVSEHVDCLTSPSLQSLSREARL
                     ALLKQSLSQAVTLEFATIPIYLAALWSIKHNLHPAAISIRNVVQEEMLHLALACNMLA
                     SIGGNPKLYDTTTNGLSYPARLPGGVHPELVLKLSGLTDDALDDFLEVELPQEEIDIE
                     EFKGKYNSGDEHHNHSQTIGALYGAISGEFKNLQPRMTPDNQVSGPLSWFVVDTPEKV
                     DSAIHWIETQGEGAIDTSPKNTGLKNLSHFYRFWEIRRRQKIEKDPDTGKFVFMSPLE
                     FPDVWPMAVVPEGGYRKEDVSEEVWLLLSKFDETYSNLMKLLESAWGAGGQAALWKAI
                     DTMFDLEKYAIPLMQIPIPEGEGNYGPCFRLI"
     gene            5521..5955
                     /locus_tag="VGB76_01835"
     CDS             5521..5955
                     /locus_tag="VGB76_01835"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0097669.1"
                     /translation="MKNKAYKNWRVVRSVELNAPAQEVWNLVGGFFNIHKWHPDIVQT
                     EVLSDQTEMSAVRRLLTFPGQPKTTEELIFMDNENFHYRYKWHEGEWGEKVQRYVAEI
                     KVLELELDQRCVVQWSSTFYYFEDALTQFYQNGFAALVKKFG"
     gene            5996..6397
                     /locus_tag="VGB76_01840"
     CDS             5996..6397
                     /locus_tag="VGB76_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097670.1"
                     /translation="MGSMVEINDTLQLTTEQGFPADLLDLKKHQKKPIKLNDVQGKIF
                     EFKDKASARIFQLDPVRVYYVHNIKGKWLFWGRVFIQSLTIYKNVDAKGNWKEGDWLT
                     SGTYKIIDIYDPSYQKTFTLHEAPPDRNYFK"
BASE COUNT         1579 a         1993 c         1986 g         1350 t
ORIGIN      
        1 gacggtgaac ttgtagaccg tgtcgggctt ggcggtgtag tcgctccaga gaaagctctg
       61 aatcggatgc tcccacgtgc tgaagcgaat ctctttccct tccgcgttta aagcccgcgg
      121 gtcggggacg actgacttga agactttcag cgaccggagc cacttcggtc ccatgttctc
      181 gccgctcaca acgtcacgcc tgaaggcgaa gcccatcagc ccctgccgcc gctcctcggc
      241 gcagtcgagc gcgatgagca cggtgtgggt tcccgcaatc gccttggccc tgaaatctcc
      301 tttagtcttg atgacacgca tcgcggactc tccctccttc aaactctact ggccttagtt
      361 cccgagcgcc tcgttcagaa gcttcgcgag ccgcacgccg ccgagcttca actgctggtc
      421 aacgacgctt ttgttggcgg aataatactc gtcggcgagc cggtagcagc cggacagttt
      481 cccggacttc acgtccttaa cgccgcagcc caggttaagg ggcgggagtt tcccgtacac
      541 cctttggacg gcggtgttgt acgactcgcc cgtccatgcg aagacgtccc cggcctgcat
      601 ctgcgccagc cccgaactcc ccccggcctt gagttcttgc aggagccttc tgtagtactg
      661 atcctccgac agattgccga cagccatcgt gtgggtgatg aagccgctgt cccagacgga
      721 gtggagattc atcggcccgt aaggcgtttt gtcgtcgccg aaccactcgg cgtatttgcg
      781 gttgccaccg acgtcgccca tgttcttcgg gtcgttgacc gccttcacgt cggccttgtc
      841 ggcgacgttg tgtaaaggtt ggtgcatgtc gccgaggatg tggacgaaaa acttcagcgc
      901 ctcggagcgg ctcgttagct cctcgcccca ctcgtgccct ttctcgttcg gaaccttctg
      961 gttcctgagg acggcctgca aacgttccag cgcctggatc gcacaatccc cccgcttcga
     1021 atccatgaca cagtcccgcg ccggctcgta cgtgaagcgt tgggggccgt ccgggttctg
     1081 catgattacg gggatgtcaa cgaagtgcca gttggcggtg tagggccgct gatccttgac
     1141 cggcgggtcg ggccacgggg cgacgcacgc cagcccttcg accagaaacg tcgcctcctc
     1201 ggccttcgtc agtttcgcct tgctcgtcag ggcgaacatg gccgggcatt tcgacttgaa
     1261 gtaggccggg ttgttgcggg cgtcattccg tagaagcttg ccgacctctg ccgcggcggc
     1321 gggcgtcagg aaactcgccg cgatgcgggc cgtgatgcgg tgcccttcgt ccccccaggc
     1381 gagggaagtg ccgggcagca tggccgtgac cccgaggcac atcaacagcg cgacaaagcg
     1441 tcccccatgg ggaagcaaat ttcttatggg cattggttct tacctccacg gcgggcgtaa
     1501 tgcgtatgcc gaaggccgct cgttgattgg ttgtggctgg attgatgagg ccaacgaggg
     1561 aagactctcg ttagcatgag cgacatttga agacgcgccg aagatattcc ttacgcgagt
     1621 gtatgtcaat aactttatgc ggcacgtctg aaggtagttg cgacggcatt aaaagcttgc
     1681 ccgccggtta agttcgtctt aaagtggcgg cacaaagttc gacgattacc gaccggccat
     1741 cgaaagggag aactctatga acgccaagtt gcgggaacat gtaagacgcg tcatgcagga
     1801 gcggggactc accgtctaca gggtcgccca gggcagcggc gagcggctca gcacagaccg
     1861 cctcgacgac ataagacagg cccgggccga aaatctttcc gacagcgaga tggaaggact
     1921 cgcgaaaggc ttgggtgagt cggcggatta cttgcgctcg ctaattgaca cgccgtagcg
     1981 gcgggcgtcg gcggcgaaat aaacatcccg gaaggtttga gggtgacggc gtgacgcctt
     2041 caccctcccc tttcatcctc acgcggttgc cgcccgcctc gaaacgaggc gcggggcagc
     2101 gccgttcgta gagactttct tcacctcaaa aataaaaggc ttgcaacgcc agggcttata
     2161 gtatataagc cctgcgtcaa tcacacgggg cgttgcggca gctgccgcgt tctctcaagt
     2221 caacgcgtct ttaccttacc taaaacaaaa ttatgattgg tggcctagtc tgcaacgacg
     2281 tagtcgagcc aggtctgttg cccggggacg gacaggaact gtctcaccgg actcgcgaat
     2341 tcctgtggcg catcttcgtc gagatcggca attgctggaa gaacgtctgg tgggagtctt
     2401 ccaacctgaa aagctcctgg ctcgatctcg tcaaggcgaa gtcagaatgc ccgcccagtt
     2461 acaacggcga gtacgtgaac gctgtgtacg tcgtacagga gttgctcgac atgtacggcg
     2521 aaccgtatgc gttctacaag ctcttcttcg agagcggcat tccgtccggc ccgccgacga
     2581 cgcgcctcgc ccacgcgaag aggtatgtcg tcgatgagtt tataagaatg caggtgctgg
     2641 cgagcggctt caagcatttc ggcggcaaga attccaacta caagggctac gtcaaaggct
     2701 cccggtacag cctcatgccg gaggtcagag agttcatcga gtcggcggag gagtccaagg
     2761 cagagtccga gtgaaggctg tttagtatga agaccaaata catccttgtg ggctccgggg
     2821 tcgccgggac aaccatcgcc aaacgccttt tagagcacga ccgcaacact tccatcctga
     2881 tgttggaggc aggcccggtc atcgaggcca aaagccgccg gggttggtgg gatcacgtca
     2941 cccttcccgg cgagcgaacg ccgtacaact acacctatga cctggactgg gaggcgaaga
     3001 caaccgggaa cgttaatttc atcagcgccg gcgcgagggt caaagcctac ggcggttcga
     3061 cgatgcactg gggggcttgg gcgttgaggt tcaagccgga ggattttcac ctttattcca
     3121 acacgggcga gggcggcaag tggccgatta cgtatgatga tttagaggag tattactgtc
     3181 aggcggaaga gtacatgtcg gtctgcggcg acgacgggga aaaatggagc cggaggagca
     3241 aaccctaccc gatgccgcca ttcaagtgga cggctgccga cggcctgatg atcaaggcgt
     3301 tccgggaggt gggcatcaag ccgggcaaga tgcccatcgc gcgctaccgc aagtgtatga
     3361 cgacgggcac ctgtaagtac tgcccgttcg gcgcgagatt cagcgcgcag tacgttctgg
     3421 acgacctcag ggaagacccc cgccaccaaa atttcatcgt ccgcccgaac tcgcccgcca
     3481 ccaagattgt ttgcagctcc cgcgaccaga tagaggccat cgagtacgtc gataaccaga
     3541 ccggcgagac gattcgggag cacgccgaca cctacatcat ctgttcgggg gcgtatgaga
     3601 ccccgaaact gttgatgaac tcgacgaacc acttttggga aaacggcgtc ggcaacgacc
     3661 acgacctgct gggccgcttc atcatctccc actccatgct gaaggttcgt gggagctcgc
     3721 cgacgaataa agagaagtgg ttccaggaat acgatttccc cactctgatg tcgcgcacct
     3781 acgacacaga ggaacagcag aagaagggga aaatattcct gttcaagaac agagtcctgc
     3841 ccaatatcga catcgcgtcg ctgatgatcg gcgggaacac gccggctcag atcgacaaga
     3901 tagtcagcgg ggagatggaa ctggaactac aagccttcat ggaagagaag ggcaagttcg
     3961 agaaccgcct gcaaatcatc cccggcaaag acaggttcgg gctgcccctc accacggtca
     4021 acttcaaccg gacggacgag gagagaaaga acacggaaga ccgcttgagc atcatgagga
     4081 aggtgattga cgccatgggg ctgaagttcg tcttcggcaa gaccgaagcc cccggcgcgc
     4141 atcacaccac gggcacctgc aagatgggaa acactcccga ggagggcgtc accgactcga
     4201 acctcaaagt ccacgacatt aaaaacctgt acctctgttc caacggggtc ttcccaaccg
     4261 gcagcgccgt caacccgacc ctgaccctgg cggcgctgtc catgaggctg acggatcacc
     4321 tgatctccca atccaatggc aactaatcaa atatgagcgt agcccaacag cagattgaga
     4381 agaaagtaga gatagtctta tctctcgaaa acccccccgt ctccgagcac gtggactgct
     4441 tgacgagccc ttcgttgcag tccctctcgc gcgaggccag gctggcgctc ctgaagcagt
     4501 ctttaagcca ggccgtcacc cttgagttcg cgaccatccc catctacctt gcggcgctct
     4561 ggtctataaa gcacaacctg caccccgccg ccatctccat cagaaacgta gtccaggagg
     4621 agatgctgca cctggcgctg gcctgcaaca tgctcgcgtc gattggcggc aaccccaaac
     4681 tctacgacac gaccacgaac ggtttgagct atcccgcgag gctccccggt ggggtgcacc
     4741 cggagctggt cttgaaactc tcggggctga cggacgacgc gctggacgat tttctggagg
     4801 tcgagttacc gcaggaagag atcgacatcg aagagttcaa gggcaaatac aattccggcg
     4861 acgagcacca caaccactca cagaccatcg gggccttgta cggtgcgatt agcggagagt
     4921 ttaaaaatct ccagcccagg atgacgcccg acaatcaggt atccggcccc ctctcctggt
     4981 tcgtggtcga cacgccggag aaggtcgaca gcgccatcca ctggatcgag acgcagggcg
     5041 aaggggccat cgatacctcg cccaagaaca cggggctgaa gaatctgtcg cacttctacc
     5101 gtttctggga gatacgcaga cggcagaaga tagagaaaga cccggacacc gggaagttcg
     5161 tctttatgtc gccgctggag ttccccgacg tctggccgat ggccgtcgtg ccggagggcg
     5221 gataccgtaa ggaagacgtc tcggaggagg tctggctgct gctgtccaag ttcgacgaaa
     5281 cctattccaa cttaatgaag cttttagaaa gcgcatgggg cgcaggcggg caggccgctc
     5341 tgtggaaggc catcgacacg atgttcgacc ttgagaagta cgccatcccc ctgatgcaga
     5401 ttccgatccc cgagggcgag ggcaactacg gcccttgctt caggttgatt taaacggacg
     5461 tgcggtcgga atttttcaat tcagcctgaa gcgaagacct cgccgacttt taaccgatac
     5521 atgaaaaaca aagcttacaa gaactggcgc gtggttcggt cggtggaact caacgctccc
     5581 gcgcaggaag tgtggaactt ggtcggcgga ttcttcaaca tacacaaatg gcacccggac
     5641 atcgtccaga cggaagttct cagcgatcag acggagatgt ccgccgtcag gcgtctgttg
     5701 acctttccgg ggcagccgaa gaccaccgaa gaactcatct tcatggacaa cgagaatttc
     5761 cactaccgct acaagtggca cgaaggcgag tggggggaga aggtgcagcg ctacgtcgcg
     5821 gagatcaagg tgttggagct tgaactcgac cagcgctgcg tcgtccagtg gtcttcgacc
     5881 ttctactact ttgaggacgc gctcacacag ttctaccaga acgggttcgc cgcgctggtc
     5941 aaaaaattcg gatgaggctt gggccgggtc gtttcaacta aggagactat aagtcatggg
     6001 aagcatggta gagatcaacg acacccttca actgacgacc gagcagggct tcccggcgga
     6061 cttgctggat ttgaagaagc accagaaaaa accgatcaag ctgaacgacg tgcaaggcaa
     6121 gatattcgaa ttcaaggaca aggcgtccgc gcgcattttt cagctcgacc ccgtccgcgt
     6181 ctactacgta cacaacatca aagggaagtg gcttttctgg gggagggtct tcatccagtc
     6241 gctgacgatc tacaagaacg tggacgcgaa aggcaactgg aaggagggcg actggctcac
     6301 ctccggcacg tacaagatta tcgacatcta cgaccccagc tatcagaaga ccttcacgct
     6361 gcacgaagcc ccgcccgaca ggaactattt caagtaacag agcgcggacg attgatgcca
     6421 cccagaatca caaaagcgca gaagcgggaa ctggttcggc aggcagcgga ggcgcttaag
     6481 aatccacacg ccaaggctta ccccaacgtc tacgccgcgg cggccatgcg tggcgcttct
     6541 gaagcttaag atgatgattg cagaacacgg tgaaatctaa atgttcaatg cggcaccttg
     6601 gacggttgaa gcctgaggat atcttttccg tgcagccctc gctgtaaata tcttccacca
     6661 cagcttatgc acagcccttc ccctatttgg gaaggacttt attgttttgg gaagaacttt
     6721 attgttcgca aacacctacg gctcactaga taaataaaca aggtagtacg aactttgtga
     6781 acaggcggta ttacgtccag taagcgcttt gaacagctcg aactgacgca aaaaggcgcc
     6841 tatccatccc caccgctttt atagctctca gcaaagaaca tcattttctc agtaaagaac
     6901 attattcc
//
LOCUS       DASWNS010000053        19213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_210062, whole genome shotgun sequence.
ACCESSION   DASWNS010000053 DASWNS010000000
VERSION     DASWNS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19213
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210062"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1486)
                     /locus_tag="VGB76_01845"
     CDS             complement(251..1486)
                     /locus_tag="VGB76_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0097671.1"
                     /translation="MPTYVFKGRNRLGEVMVGERVADSRDMLKQLLRREQVMLTSVKE
                     KGREIAVPKLSGRGKVKSKELSIFTRQFSVMIDAGLPLVQCLDILASQQENKYFQQVL
                     TQTRQDVEEGSTLAVAMSNHPKVFDQLFVNMIEAGETGGILDIILQRLSTFIEKIVKL
                     RRDVISAMIYPSAVIVIACLVVAAILIFVIPQFQTIFLGLLGPGEKLPLPTRIVVAFS
                     DFVAGWGGLCILVGLIAAGVGLRTYRKTPNGQRTTDRLLLKAPIIGPVLRKIAIARFS
                     RTLSTLLSSGVPILQSLDITARTAGNLVIEEAIRGIRTGVERGESFVEPLKATDVFPN
                     MVAQMVGIGEQTGALDSMLGKIADFYEQEVDTAIANMLTLIEPAMIGFLGVTIGGIVI
                     AMYMPLFTLVGKLSSGGGS"
     gene            complement(1626..2837)
                     /locus_tag="VGB76_01850"
     CDS             complement(1626..2837)
                     /locus_tag="VGB76_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY0097672.1"
                     /translation="MSANESSLADQVSLSDLLKKLIELGGSDLHLTTNTPPQVRVDGA
                     LKPLDGFKTLTSADTKQLAYSVLTDAQKHRFEEALELDFSFGVRGLSRFRANLFNQRG
                     AVGCVFRAIPYEIRSFENLGLPAVVTKLCDKPRGLILVTGPTGSGKSTTLAAMIDKIN
                     RERHEHIMTIEDPIEFLHNHKSCLVNQREVGSDTKGFADALRTALRQDPDVVLVGEMR
                     DLETIESALRIAETGHLTFATLHTNSAASTINRIIDVFPSNQQAQIRAQLSLVLEGIM
                     CQALLPRANGQGRVAALEILVPNAAIRNLIREDKIHQIYSMMQTGQEKYGMQTFNQAL
                     ATLYHKRLISLELAMQRSSNTDELRDLIEHGAGVNTGNGQNGAQNTPARPGAHPSPYA
                     QARPIGARPTR"
     gene            complement(2967..3926)
                     /locus_tag="VGB76_01855"
     CDS             complement(2967..3926)
                     /locus_tag="VGB76_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097673.1"
                     /translation="MFLRALVLTILIMCTVAATLPLSQSLAHWGSRPSASRRHKGRAA
                     RRHSRAWWRRYRARLRRQRAFAAERRRRRAAILTATKTKPISPFAPATNHASAKTATG
                     QASNLMLPEVTLPSTAALSSITPVTPTPMPMLAVAPTASKDASKEAARVAAPVANASA
                     LPSGWSSTASGRAGEMRFNVRAADGRVRSSAVWSRVNLQSAAPATTDARSKTFGGVPV
                     TLLRRTVINRMVAEGGWVTNDVQRQFGERRVFVVTAQSMAANGERRAWTFYFTESDGQ
                     VYSLATNAPAEFADSLATESEQAIVALGSRRNPVNSTTAKYKQ"
     gene            complement(3940..5736)
                     /gene="pilB"
                     /locus_tag="VGB76_01860"
     CDS             complement(3940..5736)
                     /gene="pilB"
                     /locus_tag="VGB76_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEY0097674.1"
                     /translation="MSAKLGEILVRENILTPQQLREALDYQREHGGRLGFNLVKLGLV
                     SDDMVTAVLSRQYGVPSVNLDLFDVDASVIRLIPRDVADKYSVLPLSRVGATLTLAMV
                     DPTNVFAMDDIKFMTGLNIEPVVVSEAGVQEAIEEYYGGTREIELASMVEATLAGSNG
                     NGHANAFAMTTDAVSLAQLDFDEEAGRDVEVIDEDDEIDVSSLARMSEDAPVVRLVNV
                     LLVDALQRGASDIHIEPYEKEMRIRLRIDGVLYDVMHPPLRLRDALISRIKIMSKLDI
                     SEKRLPQDGRIKIKLRAEGRSRELDFRVSTLPTLFGEKVVMRLLDKEKLMLDMTKLGF
                     EPESLVKFKRNISRPYGMVLVTGPTGSGKTNTLYSALQSLNTTETNIMTAEDPVEFNL
                     MGINQVQMKEQIGLNFAAALRSFLRQDPNIVLVGEIRDFETAEIAIKAALTGHLVLST
                     LHTNDAPSTISRLMNMGIEPFLVATSVNLIQAQRLIRRVCTECKEVLPTPAEALVEIG
                     FTQDEARDLKVYKGKGCAKCNNTGYKGRVGLYEVMEITDDIRELILIGASALELRKRA
                     VESGMLTLRQSGLMKIRNGVTTLEEVVRETVA"
     gene            complement(6114..7427)
                     /locus_tag="VGB76_01865"
     CDS             complement(6114..7427)
                     /locus_tag="VGB76_01865"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0097675.1"
                     /translation="MRIQKFTHFVVAALLLATFAVNASAQFTATATGKVTLKQADGTE
                     VPLPDALVEIHRTDIKQYKKVKTDKKGEYLYAGIDPTGVYTLVVSAPNATPSYIQNIN
                     LNRQSANNFSLNPGDGRTLTLAEIKAAGGAKTPTAAAAPGATGAAAAPRMSAEEAKKK
                     QEELDKEIAAVKAKNARIEERNAKLPEVFKAANEALKAKNYDLAITTYDQAIEIDPAE
                     AVVYRNKAIALRSRGVDKYNAAAKTKDQAGKESARADLKLATETMEKAITNYRTPQAD
                     RVASAPDAAKTDELDYLFQRAEAYRVALQTNAQVDTEAGIKAFQEYIASETDAAKKTK
                     AEASLGDALFQGGRVDESIAAYKAILASNPSNLDAIYGLGLALASDPTGAKTAEGRDM
                     LEQFASKAPATDPRKQMAEEAVAGLNEALKPKPADKATATKKRKT"
     gene            complement(7573..8355)
                     /gene="queC"
                     /locus_tag="VGB76_01870"
     CDS             complement(7573..8355)
                     /gene="queC"
                     /locus_tag="VGB76_01870"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY0097676.1"
                     /translation="MGGYDEKAFAELETEASEDEELSLDAEASDSSLIPHPSSLPLAV
                     CLVSGGMDSCVTAAIAASEHEELAFLHVSYGQRTQAREGRAFQELAEHYKVTKRLVAS
                     LDYLARIGGSSLTDARIPVAAANLSAREVPTSYVPFRNAHLLAIAASWAEVLRARAIY
                     IGAVAEDSSGYPDCRPEFYAAFQQAIDTGTRPETRVEIKTPVIHLRKSEIIRRGLALG
                     APLELTWSCYQAEERACGRCDSCALRLRAFREAGAVDPVPYA"
     gene            complement(8465..9112)
                     /locus_tag="VGB76_01875"
     CDS             complement(8465..9112)
                     /locus_tag="VGB76_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0097677.1"
                     /translation="MLRVTEIFRSIQGESTHAGRPCAFVRLTGCPMRCVWCDSEYTFT
                     GGERMSLGEVLAQVRAMNCQLVEVTGGEPLAQREAFALITSLCDEGYEVLIETGGYVS
                     TEKVDPRAKLILDVKCPASGEAERNHWPNLERLRADCDEIKFVVANRADWDYAREVIT
                     RYDLESRAHALLISPVWGQIDLKELADLIAASGLKKVRMQMQLHKMIWGADVHGV"
     gene            9317..10285
                     /locus_tag="VGB76_01880"
     CDS             9317..10285
                     /locus_tag="VGB76_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0097678.1"
                     /translation="MSRKATAAIATKEQLLREGTARLLGRAADARALDTGALLPRVGG
                     VLEKYLLRHQPEASASEINNFLDALRADDLCLVVACERGDEGAWSDLMEQFRPTVLSA
                     ARSASANEDAAEELAQSVWADLYGLRTRADGSAAGKLAYYSGCGSLGGWLRAVVGQLA
                     VDRHRKTARLVQTEETTDFDRLSREGHEETDHFTVSAPPDPELALAGQQTAAALERAL
                     RHTLAGLDAEDRLLIKLYYFDDLRLREAGAVLGVHEATASRRLTRLHGEVRKRVEAFL
                     ISEHGWTKQETARTLADAATHLDTDLRRMLAGAETAASERREELYE"
     gene            10388..11782
                     /locus_tag="VGB76_01885"
     CDS             10388..11782
                     /locus_tag="VGB76_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097679.1"
                     /translation="MQTEFDKEIDALLREGARRGRAASAERAQRTGEREENGGARAAS
                     SGAHLDADELNAYAENSLPPAARTYYAAHLADCDDCRRSVTQLALAAGMPSQLEQRAA
                     AATTQEVSKGVTWRERLGALFAPRAWRYAVPALALLLVGAVALLVLTRDTRQQREVSI
                     AQQQQPQPRNASEQAKPTMPETHHAAQNTNAAAGEGDPLSAANSNAPGAKEELAAVSK
                     EEQAQPGAGVILSDSTVASPPPPAPLSPGASTAAAPVTTTAAPSIPVPQATPAPVPEA
                     VITTQEAEQKLKVGGRTDELARDAQRQGNYENSSPRDRISGPRRNNDQRNMQRGVIID
                     GRDSSDKNEGASAAPAPVPPAGATAARRAKPREADGERGGRTSDDSVRSAELRASKSV
                     AETRTVAGRKFRRTGGAWVDTAYNSAQAVTVVRRNSEQYRALVADEPQLRRISDALGG
                     EVTIVWKGRAYRIR"
     gene            11998..13605
                     /locus_tag="VGB76_01890"
     CDS             11998..13605
                     /locus_tag="VGB76_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HEY0097680.1"
                     /translation="MIFRSPYPEIELPGMSLTPFVLAGARVRGDKPALIDALSGRTIT
                     YGQLFESVQRVAAGLSARGFRKGDVFAIYSPNVPEYAIAFHAVSLIGGTLTTLNPLYT
                     NEEVAHQLKDARARYLLTVPALLDRATEAAAHAHIEEIFVFGEAEGATPFAALFANEG
                     AGRGRSDEQEEVFVVPQVEIDPREDVVVLPYSSGTTGLPKGVMLTHDNLIANTLQVQA
                     CELIKESDTLVCVLPLFHIYGMVVIMNVGLYTGARIVVMSRFDLESFLKIMQDYEVTL
                     AHVVPPIMLALAQHPAVENYDLSKLQTLFSGAAPLSEKLARACSERLDCAVRQGYGMT
                     ETSPAIHLTPSTPGWSKYGSIGECVPNTECKIVDVETGEPLGYGQEGEICMRGPQMMK
                     GYLNRPEATAATIDADGWLHTGDIGYCDEEGHFFIVDRLKELIKYKGMQVAPAELEAL
                     LLTHPCVADAAVVPLADEEAGELPKAFVVRKAGAEASTPDELMAFVTAQVAPYKKIRR
                     LEFIEQIPKSASGKILRRLLVERARAK"
     gene            13644..15104
                     /locus_tag="VGB76_01895"
     CDS             13644..15104
                     /locus_tag="VGB76_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEY0097681.1"
                     /translation="MSKNLNIIRAACASIIAVFVLVACLQLAPSAHAQRRGAARRNAK
                     ASPAVATSGALRRDRDVARIISEINPRHIERTVRKLVSFGTRNTLSAQDNPTRGIGAA
                     RDWLRSEFQRIAQGSGGRLTVELQSYTQEALPPPRGRIPQPTVITNVVATLRGTHPAS
                     ASRMYVVSGHYDSMCTSPTDAVCDAPGANDDASGVAAVVEMARVMSRREFEATIVFMA
                     VAGEEQGLLGATHYAEQAKQKSLDIEAMFTNDIVGNSLGGNGVRDRRTVRVFSEGVPT
                     NETPEEATTRRGTGGENDSNARQLARFIKAVGATYLSTFNVNLIYRRDRYLRGGDHIP
                     FLERGFAAVRFTEPNEDYTHQHQNVRRENGIQYGDLPQFVDYGYIAQVARLNAAALAT
                     LARAPARPKGVGLLTRRLTNDTDLQWTANTEDDLAGYEVVWRETSSPVWTHALAVGNV
                     TSYTVKGMSKDNYFFGVRAVDRQGNRSPVSFPRPVR"
     gene            15374..17596
                     /locus_tag="VGB76_01900"
     CDS             15374..17596
                     /locus_tag="VGB76_01900"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY0097682.1"
                     /translation="MKKLFNYLAPLARRAAAFSLAFALLAGSFVALPAAASAQQKQSS
                     SASKAARLSEEQRIVHVLGRLGFGARPGDVERVRQMGLERYIEAQLQPEKIADASVEA
                     KLRNLPSYWMTTAELYRKYPQPGRLLRALERQGRLPADLAELRDNRKGGDDAAAAAPA
                     KKDAGAKMDAEGASGSETALKANGGDAPEAPAKNAARRDYRQALQEYYRENNLLQPAR
                     ITAELQSSRVLRAVYSERQLQEVLVDFWTNHFNVYANKGADRWLLVSYDRDTIRPHTL
                     GKFKDLLLATAQSPAMLFYLDNFQSVSPTAGRNANRMRPRVKRFFETLTSPQAGDAGE
                     QETPRRAARMNRRRELQREQANAQQAQQMPAQQQSQTPAPQQPRRMRRGINENYAREL
                     MELHTLGVEGSYTQKDVQEVARCFTGWTIFDPRGQGGATGLTNPERAGTFFFNPRLHD
                     DGEKLVLGHKIPAGGGVNDGLAVLDILSKHPSTAKFIATKLARRFVMDNPTPALVERI
                     SAAYTKSDGDIRTTLRAIFASPEFNAPENYRAKIKTPFELAVSAVRSLGGETTGAPAL
                     HQWIARMGEPLYQYQAPTGYPDTSEHWVNTGALLERMNFALALVGNRINGTRVDLARF
                     DGTGSTKAGADKSRLIEQFAAVILQGDMSERTKAALLKQLNEAAPAATTTNHASASNS
                     TIRTTGEMNNNDDAMMPRGGGGGRRGARREMAANLNAPISDAARIAALLLGSPEFQRQ
                     "
     gene            17751..19025
                     /locus_tag="VGB76_01905"
     CDS             17751..19025
                     /locus_tag="VGB76_01905"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0097683.1"
                     /translation="MNRRFFLKSGGVALASVGLMGASPSFLQRVAAQGLKLNAGGRRR
                     KTLIAIFQRGAVDGLNMVVPHGEKNYYALRPSIAIAQPGRGAANESVVDLDGFFGLHP
                     SLSGFKPMWDAKRLAIVHAAGSPANTRSHFDAQDYMEAGTPGVKSTPDGWLNRYLQAR
                     VDPASSPFRGVALAQNLPRSMQGRAPALAISNLADFSIRAGQYSQKVQGGFEDIYEGV
                     ANDALRGAGQETFEAIDFLKKVNPAQYRPENGAQYPRTQFGNALLQIAQLVKAGVGLE
                     VAFTEVGGWDTHVNEGGARGQLAARLSEFGNGINALYTDLGAERMDDVVILTMSEFGR
                     TVRENGNRGTDHGHANAMFIIGGATRGGRVYGDWPGLDASKLHDGRDLALTTDFRDVF
                     GEVAQRHLGNADLNRVFPGYASSPANFRGLLG"
     gene            complement(19109..>19213)
                     /locus_tag="VGB76_01910"
     CDS             complement(19109..>19213)
                     /locus_tag="VGB76_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEY0097684.1"
                     /translation="GAWSTGNVSETVVQEYLEHHRQQSNQDSDAFILE"
BASE COUNT         3681 a         5711 c         6182 g         3639 t
ORIGIN      
        1 gctcgacaag cgctttggtg agttgttgga gcaagccgtc ttgaccgagc aagtcttcgg
       61 gcggactaac tattgttgag tgtcagcggg gacgtccaac tatgcttgta ccgtagcggt
      121 aaacgcccgc acgaggattt ccctcgtacg ggcgtttact attgttgttc tcagaccaga
      181 actttgttac acagaccaga actttgttac acagaacaag actttaattc gcttggacac
      241 ttggacatct ctaagagccg ccgccgcttg agagtttgcc gacgagggtg aagaggggca
      301 tgtacatggc gatgacgatg ccgccgatgg tgacgccgag gaagccgatc atggcgggtt
      361 cgatgagggt cagcatgttg gcgatggcgg tgtcaacttc ttgttcatag aagtcggcga
      421 tcttgccgag catcgaatcg agcgcacccg tctgttcgcc gatgccgacc atctgcgcca
      481 ccatgttggg gaacacgtcg gtcgccttca gcggttcgac gaaactttcg ccgcgttcga
      541 cgccggtgcg gatgccgcgg atggcttctt cgataacgag gttgccggcg gtgcgagccg
      601 tgatgtcgag cgattggagg atggggacgc cggaggagag cagggtggag agcgtgcgcg
      661 agaagcgtgc gatggcgatc ttgcgcagga cgggtccgat gatcggggct ttcaaaagca
      721 gtcgatcagt ggtgcgctga ccgttcgggg tcttgcgata ggtgcgtagg ccgacgccgg
      781 cggcaatgag gccgacgagg atgcagaggc cgccccaacc ggcgacgaaa tcgctgaagg
      841 cgacgacgat gcgcgtgggg agcggaagct tttcgccggg tccgaggagt ccgaggaaga
      901 tcgtctggaa ctgcggaatg acgaagatga gaatcgcggc cacgaccaga cacgcaatga
      961 cgatcacagc cgaaggatag atcatcgcgg agatgacatc gcgtcgcagt ttgacgatct
     1021 tttcgatgaa ggttgacagg cgttgcagga tgatgtcgag aataccgccc gtctcacccg
     1081 cttcgatcat gttgacgaag agttgatcga acactttggg atggttcgac atggcgaccg
     1141 ccagcgttga cccctcttcc acgtcctgcc gggtttgcgt caagacttgc tgaaaatatt
     1201 tgttctcctg ctggctcgcg agaatgtcca gacattgcac gagcggcagc cccgcatcga
     1261 tcatcacgga gaattgacgc gtgaagatcg ataactcttt agacttcact ttgccgcgcc
     1321 cgctcaactt cggcacggcg atctcgcgcc ccttctcttt gacggaggtg agcatgacct
     1381 gttcgcgccg caacagttgt ttcaacatat cgcggctgtc ggcgacgcgt tcgccgacca
     1441 tcacttcgcc gagtcggttg cggcctttga agacataagt gggcatctgt gaaaactcct
     1501 taagaacagt gacaagtgac aagtgacgag ttaagacttg tagatcgtga ataaaaagtc
     1561 tgtcttcgtt gcgtgacgat tgacggcttt tgcttgttac tcgtcacccg tcacttgtcc
     1621 ctgctttatc ttgtgggacg tgcgccgatg ggacgcgcct gtgcgtaggg gctgggatgc
     1681 gcgccggggc gggcgggcgt gttctgcgcg ccgttctggc cgttgcccgt gttgacaccc
     1741 gcgccgtgtt cgatcagatc gcgcagttcg tccgtgttgg acgaacgctg catggcgagt
     1801 tcgagcgaga tcaggcgttt gtgatagagc gtggcgagcg cctgattgaa agtctgcatg
     1861 ccgtactttt cctgacccgt ctgcatcatc gagtagatct gatgaatctt gtcttcgcgg
     1921 atcaggttgc ggatggccgc attggggacg aggatttcca gcgcggcgac gcgaccctgc
     1981 ccgttcgcgc gcggcaggag cgcctgacac atgatgcctt cgagcacgag cgacaactgc
     2041 gcgcgaatct gcgcctgctg gttggaaggg aacacgtcga tgatgcggtt gatggtggaa
     2101 gcggcagagt tcgtgtgcag ggtggcgaaa gttaagtggc cggtttcggc gatgcgcagg
     2161 gctgattcga tggtttcgag gtcgcgcatt tcgccgacga gcactacgtc ggggtcttgc
     2221 cggagcgcgg tgcgcagggc gtcggcgaag cctttggtgt ccgagccgac ttcgcgctgg
     2281 ttgacgaggc aggacttgtg attgtgcaga aactcgatgg ggtcttcgat ggtcatgatg
     2341 tgctcatgac gttcacgatt gatcttatcg atcatcgcgg cgagcgtggt cgatttaccg
     2401 gagccggtcg gtcccgtgac gagaatgagt ccgcgcggct tgtcgcagag cttggtgacg
     2461 accgccggga ggccgaggtt ctcgaacgac ctgatctcgt aggggatggc gcggaagacg
     2521 cagccgaccg cgccgcgctg gttgaagagg ttggcgcgga aacgggagag gccgcgcacg
     2581 ccgaacgaga aatcgagttc gagcgcttcc tcgaagcgat gcttctgcgc gtccgtgagc
     2641 acgctgtagg cgagctgctt ggtgtccgcg gaggtgagcg tcttgaagcc gtcgagcggt
     2701 ttgagcgcgc cgtcgacacg cacctgcgga ggggtgttgg tggtcaggtg taaatccgag
     2761 ccgcccagtt cgatcaattt tttgagcaga tcggagagcg agacttgatc cgccagtgaa
     2821 gattcgttag ctgacatttt tctttatgaa ctccgtgggg aagattgccc gaattgcgga
     2881 gggcgagcgc gccgagcggt gctggctgct attgctgcgg cgcgtctgaa aaagtggttt
     2941 cgcgcgatga gcgaagaatt agacgctcac tgtttgtatt tggcggtcgt cgagttgacg
     3001 gggttgcggc gtgagccgag cgcgacgatg gcctgctcgg actcggttgc gagcgagtct
     3061 gcgaattcgg cgggcgcgtt cgtcgcaagg ctatagacct gcccgtcgga ttccgtgaag
     3121 tagaaggtcc aggcgcggcg ttcgccgtta gcggccatgc tctgcgcggt gacgacgaac
     3181 acgcggcgtt cgccgaactg gcgttgcacg tcgttcgtca cccagccgcc ctcggccacc
     3241 atccggttga tcaccgtgcg acgcaggagc gtgacgggga cgccgccgaa agttttgctg
     3301 cgcgcgtcgg tcgtggcggg cgcggcggat tgcaagttga cgcgcgacca gacggctgag
     3361 gagcgcacgc gtccgtctgc ggcgcgcacg ttgaaacgca tttcacctgc gcgcccggaa
     3421 gccgtgctgc tccaaccgct cggcaaagca gaggcgttgg cgaccggagc cgctacgcgc
     3481 gccgcctctt tcgacgcatc tttcgacgcc gtaggcgcga ccgcgagcat gggcatcggg
     3541 gtcggcgtca cgggagtgat gcttgagagc gcggcggtgc tcggcagagt cacctcgggc
     3601 agcatgagat tgctcgcctg acccgtggcc gtcttcgcgc tcgcatggtt cgtggcgggc
     3661 gcgaaggggg agatgggctt ggttttggtc gccgtcaaga tggcagcgcg acggcgtcgg
     3721 cgttcggcag cgaaggcgcg ctggcgtcgg aggcgcgcac ggtagcgtcg ccaccaggcg
     3781 cgagagtggc ggcgcgcggc gcgtcctttg tgacggcgcg aggcggaggg gcgcgacccc
     3841 caatgcgcca gcgattgcga gagcggcagc gtcgccgcca ccgtgcacat gatgagtatc
     3901 gttaaaacta aagcgcgcaa aaacattggg aacctctctt taagcgaccg tctcgcgcac
     3961 gacctcttcg agggtggtca cgccgtttct aatcttcatc aatcccgatt gacgcagggt
     4021 gagcatcccg ctttcaaccg cacgcttgcg cagttcgagc gccgacgcgc cgatcaggat
     4081 caattcgcgg atgtcgtccg tgatctccat cacttcgtaa agaccgaccc ggcctttgta
     4141 gccggtgttg ttgcacttcg cacagccctt gcctttgtag accttgaggt cgcgcgcttc
     4201 gtcctgcgta aagccgattt cgacgagagc ttcggcgggc gtcgggagca cttctttgca
     4261 ttccgtgcag acgcggcgaa tgaggcgttg cgcctgaatc aggttgacgg aggtggcgac
     4321 gaggaaaggc tcgatgccca tgttcatcag gcgggagatg gtggagggcg cgtcgttggt
     4381 gtggagggtg gagagaacga ggtgcccggt gagcgcggcc ttgatggcga tctcggcggt
     4441 ttcgaagtcg cggatttcgc cgacgaggac gatgttcggg tcttggcgca ggaaggaacg
     4501 cagcgcggcg gcgaagttca aaccgatctg ctctttcatc tgcacctgat tgatgcccat
     4561 caggttgaac tcgaccgggt cttccgccgt catgatgttc gtctcggtcg tgttcagaga
     4621 ctggagcgcg gagtagagcg tgttggtctt gcccgaaccg gtcggtcccg tgacgagcac
     4681 catgccgtag gggcgcgaga tgttgcgctt gaacttgacg agcgattccg gctcgaagcc
     4741 gagcttcgtc atgtcgagca tcagcttctc tttatcgagg agacgcatca cgactttttc
     4801 gccgaacagc gtcggcagcg tcgagacgcg gaagtccaac tcgcgtgaac gaccttcggc
     4861 gcgcagcttg atcttgatgc gcccgtcctg cggcagacgt ttctccgaga tgtcgagctt
     4921 cgacatgatc ttgatgcgcg agatgagcgc gtcgcgcagg cgaagcgggg ggtgcatcac
     4981 gtcgtagagc acgccatcaa tgcgcaggcg aatacgcatc tctttttcgt aaggctcgat
     5041 gtgaatatcg gaagccccgc gttgcagcgc gtccacgagc aggacgttga cgagacgcac
     5101 gacgggcgcg tcctcgctca tgcgcgccag gctcgacacg tctatctcgt cgtcttcgtc
     5161 aataacttca acgtcccggc cagcctcttc gtcgaagtcc aactgtgcga gcgagacagc
     5221 gtccgtcgtc atggcaaagg cgttagcgtg gccgttgccg ttcgatccgg ccagcgtggc
     5281 ctcaaccatg ctcgccagtt cgatctcgcg cgtcccgccg taatattcct cgatggcctc
     5341 ctgcacgccc gcttccgaga cgacgactgg ctcgatgttc aagcccgtca taaacttgat
     5401 gtcgtccatg gcgaagacgt tcgtggggtc aaccatcgcc agcgtcaaag tcgccccgac
     5461 gcgcgagagc ggcagcaccg aatatttgtc ggcaacatcg cgcgggataa ggcggataac
     5521 cgacgcgtca acgtcgaaca ggtcgagatt gacgctcggc acgccgtact ggcgcgagag
     5581 cacagccgtc accatgtcgt cggagacaag gccgagtttg acgaggttga agccgagacg
     5641 cccgccgtgc tcgcgttggt agtcgagcgc ttcgcgtagc tgctgcggcg tcaggatatt
     5701 ttcacggacg agaatttcgc ctaatttagc tgacatttct tgactgtttc cacagacttc
     5761 ggacgctgtt agggaagaat ttgagacgag gcaggcactt caattaactc ggataaagga
     5821 gatgtacgcg agacgcgtgc tgtttgtctt aaggcaacga tggcaaacat cgttggcaaa
     5881 atcatgttaa cggacgagtc gtgtggctgt caagatgaat atgcgttgac gcgccggaag
     5941 gttgaaagga gtgcgaaaag gcgtatttca ggccgatttt taaatagaga gggcgcgtcg
     6001 cggttgtcaa attgaacgtt ggaggggcgt gaggcggggt gggggagggt ttaaaggcga
     6061 acgggcgacg cctgtttgag cgtcgcccgt tcgtttttga gattgaaaag agtttaggtt
     6121 ttgcgttttt tggtggcggt cgctttgtca gccggtttgg gcttgagagc ttcgttgagt
     6181 ccggcgacgg cctcttccgc catctgcttg cgcgggtcgg tggcgggagc tttgctggca
     6241 aattgttcga gcatgtcgcg tccttcagcc gtcttcgcgc cggtcgggtc ggaggctaag
     6301 gcgaggccga ggccgtagat ggcgtcgagg ttgctcgggt tgctagccag aatcgccttg
     6361 taggccgcga tggactcgtc cacgcgcccg ccctgaaaca gcgcatcgcc gaggctcgcc
     6421 tccgctttgg tcttcttcgc cgcgtcggtt tcagaggcga tatactcctg aaacgccttg
     6481 atgcccgctt ctgtatcgac ctgcgcgttc gtttgcagcg cgacgcgata agcctctgcg
     6541 cgctggaaca ggtagtctaa ctcgtcggtc ttcgccgcgt cgggcgcgga ggcgacgcgg
     6601 tcggcttgcg gcgtccggta gttggtaatg gctttctcca tcgtctcggt ggcgagtttc
     6661 aaatcggcgc gcgcagactc tttgcccgcc tgatctttag tcttcgcggc ggcgttgtat
     6721 ttgtccacgc cgcgcgaacg gagcgcgatg gccttgttgc gatagacgac ggcttcggcg
     6781 gggtcaatct ctatcgcctg atcgtaggtg gtgattgcca gatcatagtt cttggccttg
     6841 agggcttcgt tcgccgcctt gaagacttcg gggagtttgg cgtttcgctc ttcgatcctc
     6901 gcattcttgg ctttgaccgc agcgatctct ttatccaact cctcctgttt cttcttcgct
     6961 tcttccgcgc tcatcctcgg cgcggcggct gccccggttg cgcccggtgc ggcggcagcg
     7021 gtcggagttt ttgcgccgcc tgcggccttg atctcggcca gcgtcagcgt cctgccgtcg
     7081 ccggggttga gcgagaagtt gttggccgac tgacggttga gattgatgtt ctggatataa
     7141 ctcggagtcg cgttcggcgc gctgacgacg agggtgtaga cgccggtcgg gtcgatcccg
     7201 gcgtagaggt attcgccctt cttatccgtt tttactttct tgtattgttt aatatcggtg
     7261 cggtgaatct ccacgagcgc atcggggagc gggacttccg tgccatcggc ctgcttgagc
     7321 gtaaccttgc cggtggcggt ggccgtgaac tgtgccgagg cgttgacggc gaaggtcgcc
     7381 aagagcaacg cggcgacgac gaagtgtgtg aatttttgga tacgcatatt gggcttctcc
     7441 tgtaaatgat tctgtaggga gtttaattat aaaggggaat tcaatcaagt cttcagccgc
     7501 ccgttaagat gcgtgcggag gctggccggt tgtcatcgtt cgtctcacct caataccagc
     7561 tcgacgggca aatcaagcat acggaacagg atcgaccgcg cccgcctccc gaaacgcgcg
     7621 caggcgcagg gcgcacgaat cgcaacgccc gcaggcgcgc tcctccgctt gataacaaga
     7681 ccaggtgagt tcgagcggcg cgccgagtgc gaggccgcgc cggatgatct cggacttgcg
     7741 caggtgtatg acgggcgttt tgatctcgac gcgcgtttcg gggcgcgtcc ccgtgtcgat
     7801 ggcctgctgg aaagccgcgt aaaactccgg tcggcaatct ggataaccgg aagagtcttc
     7861 ggccaccgcg ccgatgtaga tggcacgcgc acgcagcact tccgcccaac tcgcggcgat
     7921 ggcgagcagg tgagcgttgc gaaaaggcac ataactcgtc ggcacttcgc gtgcgctcag
     7981 gttggccgcc gcgaccggaa tccgcgcatc cgtcagagac gaaccgccga tgcgcgccag
     8041 gtaatcgagc gaggcgacga gtcgctttgt cactttgtaa tgctccgcaa gttcctgaaa
     8101 agcacgcccc tcgcgcgcct gtgtgcgctg gccataagag acgtggagaa aagccaactc
     8161 ctcatgctcc gacgccgcga tggccgccgt cacacatgag tccatgccgc cggagacgag
     8221 acacacggcc agtggaaggg atgagggatg agggatgagg gatgaatcag aagcctccgc
     8281 gtcaaggctc aactcttcgt cctcagacgc ttccgtctcc aattcggcaa acgctttttc
     8341 gtcgtatccg cccataagtt tcagggatga aggatgaggg atgagggatg aataagaaaa
     8401 gcactcaagt gagttgaata gttctctgat tcatccttcg tccctcatcc tttatccctt
     8461 cctttcagac tccgtgaacg tccgcgcccc agatcatttt gtggagctgc atctgcatgc
     8521 gaactttttt gaggccgctc gcggcgatga ggtcggccaa ctctttcaga tcaatttgac
     8581 cccagacggg cgagatcagg agcgcgtgtg cgcggctttc gagatcgtag cgtgtgatga
     8641 cttcgcgcgc gtagtcccag tcggcgcgat tggcgacgac gaatttgatc tcgtcgcaat
     8701 cggcgcgcaa gcgttcgagg ttcggccagt gattgcgttc ggcctcgccg gaggcagggc
     8761 atttcacgtc gaggatcaac ttcgcgcgcg ggtcaacctt ctcggtcgag acgtagccgc
     8821 ccgtctcaat caggacttcg tagccttcgt cgcagaggct ggtgatgagc gcgaaggctt
     8881 cgcgttgcgc gagcggttcg ccgcccgtca cttcgacgag ttggcagttc atcgcgcgca
     8941 cctgtgcgag cacctctccg agcgacatgc gctcgccgcc cgtgaacgtg tactcgctat
     9001 cgcaccacac gcagcgcatc gggcagcccg tcagccgcac gaacgcgcac ggtcgcccgg
     9061 cgtgcgtaga ctcgccttga atcgaacgaa aaatttccgt tacgcgaagc atgagaatcg
     9121 aaatgctgcc tgttgtccgt cgcccttagt caatagtcaa tcgccgatgg tcagtcgttc
     9181 aggccaattt atagcagctt gtgtgacagg cacaaacgac ggacaattga ccgctgacaa
     9241 cggacacaga acaactgaca aatctttacc ttgtgttagc atacgcgctt gacgggctgg
     9301 gcatcaaatc caagcgatga gccggaaggc gacagcagcc atagcaacta aggagcaact
     9361 tttacgcgag ggcacggcgc gcctgctcgg gcgtgcggct gacgcgcgtg cgctcgatac
     9421 gggcgcgctc ctgccgcgcg tgggcggcgt gctggagaaa tacctgttgc gccaccagcc
     9481 ggaagcgtcg gcctcggaga tcaataattt tctcgacgcg ctacgcgcag acgatctgtg
     9541 tctcgtcgtg gcctgcgagc gcggcgacga aggggcgtgg agcgatctga tggagcagtt
     9601 tcgcccgacg gtgctctcgg cggcgcgcag cgcatcggcg aacgaagatg cggcggagga
     9661 gttggcgcag tccgtctggg ctgacctcta cggcctgcgc acgcgcgccg acggaagcgc
     9721 ggcggggaag ctggcctatt attccggttg cggttcgctc ggcggttggc tgcgcgccgt
     9781 cgtcggccaa ctcgccgttg accgtcatcg taagacggcg cgactcgtgc agaccgaaga
     9841 gacgaccgac ttcgaccgtc tctcgcgcga ggggcatgaa gagacagacc acttcaccgt
     9901 gagcgcgccg cccgacccgg aactcgcgct cgcagggcag cagacggcgg ctgcgctcga
     9961 acgcgcgctc aggcatacgc tcgccggact cgacgcggaa gatcgtctcc tgatcaaact
    10021 ttactacttc gacgatctgc gcctgcgcga agcgggcgcg gtgctcggcg tacacgaagc
    10081 gacggcgagc cgtcgtctga cgcgtctgca cggagaggtg agaaagcgcg tcgaggcgtt
    10141 tttgatctcc gaacacggtt ggacgaaaca ggagacggcg cgcacgctcg cagacgccgc
    10201 cacgcatctc gacacagacc tccggcgcat gctcgccgga gcggaaactg ctgcgagcga
    10261 gcggcgcgaa gaactctatg aatgaatcgc atgcggtcaa ccagcacgcg cgcccggcgc
    10321 aagaaaaggg cacgcggcgc gttcttcaaa tggccggaag ttgttctaaa gttcgactgg
    10381 aagtttaatg caaacagagt tcgacaaaga gatagatgca ctgttgcgcg agggcgcgcg
    10441 gcgcgggcgt gccgcatccg ccgaacgcgc gcagcgcacg ggcgagcgcg aagagaacgg
    10501 cggcgcgcgg gcggcctctt ccggcgcgca cctcgacgcg gatgaactga acgcttatgc
    10561 ggaaaactcc ctgccgcccg ctgcgcgcac gtattacgca gcgcatctgg cggattgcga
    10621 cgattgccgc cggagcgtca cgcagcttgc gctcgctgcc gggatgcctt cgcagttgga
    10681 acagcgcgcg gctgcggcca ccacacagga agtctcgaag ggcgtcacgt ggcgcgaacg
    10741 cctcggcgcg ttgtttgcgc cgcgcgcgtg gcgttacgcc gtgcccgctc tcgcgttgtt
    10801 actcgtcggc gcggtggcct tgttggtgtt aacgcgcgac acgcggcagc agcgtgaggt
    10861 gtctatcgcg cagcagcaac agccgcagcc gcgcaacgca tcggagcagg cgaaaccgac
    10921 gatgccggag acgcaccacg cggcgcagaa cactaacgcc gccgctggcg aaggcgaccc
    10981 gctatcggct gccaactcga atgcgccggg ggcgaaagaa gaactcgctg ccgtgagtaa
    11041 agaggagcag gcgcagccgg gggcgggcgt gatcttgagc gacagcaccg tcgcgtcgcc
    11101 tccgccgccc gcgccgcttt cgccgggcgc gtcaacggcg gctgcgcccg tcactactac
    11161 cgccgccccg agtattccag tgccgcaggc gacgcccgcg cccgtcccgg aggcggtcat
    11221 caccacacag gaggccgagc agaaactcaa ggtcggcgga cgcaccgacg agttggcgag
    11281 ggatgcacag aggcagggca actacgaaaa cagttctccg cgtgatcgga ttagcggtcc
    11341 acggcgcaac aacgatcaac gcaacatgca gcgcggggtc atcatcgacg ggcgcgacag
    11401 tagcgacaag aatgaaggag cctctgccgc gcctgcgccc gttccgcccg ccggagccac
    11461 cgccgcgcga cgcgcgaagc cgcgcgaagc ggatggagag cgtggtggca gaacgtccga
    11521 tgattccgtg agaagcgcag aactgcgcgc gtcgaagtcc gtcgcagaga cgcgcacggt
    11581 cgccggaaga aagtttcgcc gcacgggcgg cgcgtgggtt gacacggcct ataactctgc
    11641 gcaggcggtc acggtcgtcc ggcgcaactc cgaacagtac cgcgcgctcg tcgcagacga
    11701 accgcaactg cgccgcatca gcgatgcgct cggcggcgaa gtcaccatcg tctggaaagg
    11761 gcgcgcctac cgcatccgtt aacaagaaca gtgacgagtg acaagtgacg agcgaagggt
    11821 ggcgggcgac aggtgaaaaa tatttacctg ccgcccgctt ctcatgtaag gtgtacccct
    11881 caagagccgc ttcggctgac aactaactac aagcgaagat actgcgcgag gtttaaatca
    11941 cgaatttgaa cttgtcacct gtcactcgtc actcgtcact gtctttggag gtcttcgatg
    12001 atcttccgca gcccctatcc tgagatcgag ttgccgggca tgtcgctcac gccgtttgtg
    12061 ctggcgggcg cgcgtgtgcg cggagacaag cccgcgttga tcgatgcctt gagcgggcgc
    12121 acgatcactt acgggcagct tttcgagtcg gtgcagcgcg tcgccgccgg actctctgcg
    12181 cgcggctttc gcaaaggcga tgtcttcgcc atctacagcc cgaacgtccc tgagtatgcc
    12241 atcgcttttc acgccgtctc actcatcggc gggacgctca cgacgctcaa cccgctctac
    12301 acgaacgaag aagtcgcgca tcaactcaag gacgcacgcg ccaggtatct gctcaccgtg
    12361 cctgcgctgc tcgacagggc gaccgaggcc gccgctcacg ctcacatcga agaaattttt
    12421 gtgttcggcg aagcggaggg cgcaacgcct ttcgccgcgc tctttgcgaa cgaaggggcg
    12481 ggcagaggga ggagcgacga gcaggaagaa gttttcgtcg tcccgcaggt cgaaattgat
    12541 ccgcgtgagg atgtggttgt gcttccgtat tcgagcggga cgacggggct gccgaagggc
    12601 gtgatgctca cgcacgataa tttgatcgca aacacgctgc aagtgcaggc gtgcgagttg
    12661 atcaaggagt cggacacgct cgtctgcgtg ctgcccctgt ttcacatcta cggcatggtc
    12721 gtcatcatga acgtcggcct ctacacgggc gcgcggatcg tcgtcatgtc gcgcttcgat
    12781 ctcgaatcgt tcctcaagat catgcaggac tacgaggtca cgctcgcaca cgtcgtcccg
    12841 ccgatcatgc tcgcgctcgc gcagcatcct gcggtggaaa actacgacct ctcaaaactt
    12901 cagacgctct tctccggcgc ggctcccttg agcgaaaaac tcgcgcgggc gtgcagcgag
    12961 cgtctcgact gcgccgtccg tcagggctac ggcatgacgg aaaccagccc ggccatccat
    13021 ctcacgccct ccacgcccgg ctggtcgaag tacggctcaa tcggcgagtg cgtgccgaac
    13081 accgaatgca agatcgtgga cgtggagacg ggcgagcctc tgggctacgg gcaggagggc
    13141 gaaatctgca tgcgcgggcc gcagatgatg aagggctatc tgaaccgccc cgaagccacc
    13201 gccgcgacga ttgacgcaga cggctggctg cacacgggcg acatcggcta ttgcgatgag
    13261 gaaggccatt tcttcatcgt ggatcgcctg aaagagttga tcaaatataa aggtatgcag
    13321 gtggcaccgg ctgaactcga agcgttgctg ttgacgcacc cctgcgtcgc cgacgcggcg
    13381 gtcgtgcccc ttgcggacga ggaagcgggc gaactgccga aagccttcgt cgtacgcaaa
    13441 gcgggagcgg aggcttcgac gcccgacgag ttgatggcct tcgtcaccgc gcaggtcgcg
    13501 ccctacaaaa agattcgccg actcgaattc atcgagcaga ttcccaagtc ggcctccggc
    13561 aaaatcctgc gccgcctgct cgtcgagcgc gcgcgcgcaa agtaaatctc gaacactcaa
    13621 gaacgcgcga ggggcaaatt ctcatgtcga aaaacttaaa catcatacgc gccgcgtgcg
    13681 cgtcgatcat cgccgttttc gttctcgtcg cctgcctcca actcgcgccc tccgcacacg
    13741 cgcagcgaag aggcgcggcg cgcaggaacg caaaagcttc tccggctgtt gccacctccg
    13801 gcgcgttgcg ccgcgaccgc gatgtcgcgc gcatcatctc ggagatcaac ccgcgccaca
    13861 tcgagcgcac ggtgcgaaag ctcgtctcct tcggcacgcg caacacgctc tcggcgcagg
    13921 acaaccccac gcgcggcatc ggcgcggcgc gcgactggct caggagcgag tttcagcgga
    13981 tcgcgcaagg gtcgggcggg cggctcacgg tcgagttgca gtcttacacg caggaagcgt
    14041 tgccgcctcc gcgcgggcgc atcccgcaac cgaccgtcat cacgaatgtc gtggcgacgc
    14101 tcagaggcac gcatcccgcg tcggcctcgc gcatgtacgt cgtcagcggt cactacgatt
    14161 cgatgtgcac cagcccgacg gatgccgtgt gcgatgcgcc gggggcgaat gatgatgcgt
    14221 caggtgtggc ggcggtggtg gagatggcgc gcgtcatgtc gcggcgcgag tttgaggcga
    14281 cgattgtgtt catggcggtg gcgggcgagg agcagggctt gctcggcgcg acgcattacg
    14341 cggagcaggc caaacagaag agccttgaca tcgaagcgat gtttacgaac gacatcgtgg
    14401 gcaatagctt gggcggaaac ggcgtgcgcg accggcgcac ggtgcgcgtc ttctccgaag
    14461 gcgtgcccac gaacgagacg cccgaagagg ccacgacgcg gcgcggcacg ggcggcgaaa
    14521 acgattccaa cgcacgccaa ctcgcacgct tcatcaaagc cgtcggcgca acctacctgt
    14581 ccaccttcaa cgtcaacttg atctaccggc gcgaccgcta cctgcgcggc ggcgaccaca
    14641 tcccgtttct cgaacgcggc ttcgcggcgg tgcgcttcac cgagccgaac gaggattaca
    14701 cgcaccagca tcaaaacgtc cgccgcgaaa acggcatcca gtacggcgac ttgccgcagt
    14761 tcgtggacta cggctacatc gcacaggttg cgcgcctgaa cgccgccgcg ctcgccacgc
    14821 tcgcacgcgc ccccgcgcgc ccgaaaggcg tcggcctgct cacgcgccgc ctcacgaacg
    14881 acacagacct ccaatggacg gcgaacaccg aagacgatct ggcgggctac gaagtggtct
    14941 ggcgcgagac gagttcgccc gtctggacgc acgcgctcgc ggtgggcaac gtaacgagtt
    15001 atacggtcaa agggatgtcg aaggataact actttttcgg cgtgcgcgcc gtggataggc
    15061 agggcaatcg cagccccgtc agcttcccgc gcccggttcg gtaaagggat gaaggatgag
    15121 ggatgaggga tgaataagaa aaccgctcaa ccgggtgcgt gcttcttctg attcatccct
    15181 catccctcat ccctcatcct tcatccttca tccctcattc ttcatccctt gctttcccgc
    15241 ctctaatttg ttcaaccatt tcatgctgcg gcgcgtctaa atcaccgggt catttaaagt
    15301 ctaaaagatt gtcccggtct gaagagattc aggcgaatat tcaatccggc aaagttttat
    15361 caagggaaat tttatgaaga agctttttaa ttatctcgct ccgctcgcac gacgcgcggc
    15421 ggcgttctcg ctcgcgttcg cgttgctcgc cgggtcgttc gtcgcgttgc ctgccgccgc
    15481 gagcgcacag cagaagcagt cttcgtctgc gtcgaaggcg gcgcgtctga gcgaggagca
    15541 gcgcatcgtg catgtgctcg gtcgcctcgg tttcggcgcg cgtcccggcg acgtggagcg
    15601 cgtgcgccag atggggcttg agcgttacat cgaggcgcaa ctccaacccg aaaagatcgc
    15661 cgacgcgagc gtggaagcga agctgcgcaa tctgccgtct tactggatga cgaccgccga
    15721 gctttatcga aaatacccgc agccggggcg actcctgcgc gcgttggagc ggcagggacg
    15781 cctgcccgca gacctcgccg agttgcgcga taatcgcaag ggcggcgacg acgcggcggc
    15841 agccgctccc gcgaaaaaag atgcgggcgc gaagatggac gcggaaggcg cgagcgggag
    15901 cgagacggcg ttgaaggcga acggcggcga cgcgcccgaa gccccggcga agaacgccgc
    15961 gcgcagagat tatcgtcagg cgctacagga gtattaccgg gagaacaatc tgctacagcc
    16021 cgcgcgcatc actgcggagt tgcaatcgtc gcgcgtcctg cgcgcggtct acagcgagcg
    16081 gcagttgcag gaagtcctcg tcgatttctg gacgaaccat ttcaacgtct atgccaacaa
    16141 gggcgcggat cgctggctcc tcgtctctta tgaccgcgac acgatccgcc cgcacacgct
    16201 cggcaagttt aaagacctgc tgctggcgac ggcgcaaagc cccgcaatgc ttttttatct
    16261 cgacaacttt cagagcgtca gcccgaccgc cggacgaaat gctaaccgca tgcgcccgcg
    16321 cgtcaaacgt ttcttcgaga ctttaaccag cccgcaggcg ggcgacgccg gggaacagga
    16381 aacgccgcgc cgcgccgcgc gcatgaaccg gcggcgcgag ttgcagcgcg aacaggcgaa
    16441 cgcgcagcaa gcgcagcaga tgcctgcaca acagcaatct caaacacccg cgccgcagca
    16501 accgcgtcgc atgcgtcgcg gcatcaacga aaactacgcg cgcgagttga tggagttgca
    16561 cacgctcggc gtcgagggca gctacacgca gaaggatgtg caggaagtgg cgcgttgctt
    16621 caccggctgg actatcttcg acccgcgcgg gcagggcggc gcgacgggct taacgaaccc
    16681 cgaacgcgcc gggacgttct tcttcaatcc gcgcctgcac gacgacggcg agaaactcgt
    16741 gctcggccac aagattcccg cgggtggcgg cgtcaacgac ggtcttgccg tgctcgacat
    16801 tctctcgaag catccctcga cggcgaagtt catcgccacg aaactcgcgc gccgcttcgt
    16861 catggacaac ccgacgcccg cgttagtcga acgcatcagc gcggcctaca cgaagtctga
    16921 cggcgacatc cgcaccaccc tgcgcgccat cttcgcctcg cccgaattca acgcgccgga
    16981 gaactaccgc gcgaagatca agacaccttt cgaactcgcc gtcagcgcgg tgcgttcgct
    17041 cggcggcgag acgacgggcg cgcccgcctt gcatcaatgg atcgcgcgca tgggcgagcc
    17101 gctctatcaa tatcaggctc cgaccggcta cccggacacc tccgagcact gggtcaacac
    17161 gggcgcgctg ctcgaacgca tgaacttcgc cctcgcactc gtcggcaacc gcatcaacgg
    17221 cacgcgcgtc gatctcgcac gcttcgacgg cacgggcagc acaaaggcgg gcgcggacaa
    17281 gtcgcgcctc atcgaacagt ttgccgccgt catcttgcag ggcgacatgt cggaacgaac
    17341 gaaggccgcc ctgctcaagc agttgaacga agccgctccc gcagccacga cgacaaacca
    17401 cgcgagcgcg tcgaactcaa ccatacggac gacaggcgag atgaacaaca acgacgatgc
    17461 gatgatgcca cgcggcggtg gcggcgggcg tcgcggcgcg cgacgcgaga tggctgccaa
    17521 cctcaacgca ccgatctccg acgcggcgcg catcgccgca ttgctcctcg gctcgcccga
    17581 gtttcaaagg cagtgacaag tgaccagtga cgagataaaa cagtgataag tgacaggtga
    17641 caagtgacga gacggaggac gagtttaaag gcaagcttga agacagagct tttgttttaa
    17701 cttgtcacct gtcactggtc acccgtcact gatttagaga ggttatgaag atgaatcgta
    17761 gatttttttt gaagtcgggc ggcgttgcgc tggcgagcgt ggggttgatg ggagcgtcgc
    17821 cgtcgttttt gcagcgcgtg gcggcgcagg gtttgaaatt gaacgcgggc gggcgacgca
    17881 gaaagactct gatcgctatc ttccagcgcg gcgcggttga cggcctcaac atggtcgtgc
    17941 cgcacggcga gaaaaactat tacgcgctgc gtccttcaat cgccatcgcg caaccgggca
    18001 gaggcgcggc taacgagtcg gtcgttgatc tcgacgggtt cttcggcctg cacccgtcgc
    18061 tctccggctt caagccgatg tgggatgcga agcgtctcgc catcgtgcac gccgcaggct
    18121 cgcccgccaa cacgcgctct cacttcgacg cgcaggacta catggaagcg ggcacgcccg
    18181 gcgtcaagtc aacgcccgac ggctggctca atcgttactt gcaggcacgc gtcgatcccg
    18241 cgtcttcgcc gtttcgcggc gtcgcgctcg cgcagaacct gccgcgctcg atgcagggac
    18301 gcgcgcccgc cctcgccatc tccaacctcg cggacttctc gatccgcgcc ggtcaatatt
    18361 cgcagaaggt gcagggcggc ttcgaggaca tctacgaagg ggtggcgaac gatgcgctgc
    18421 gcggcgcggg gcaggagact tttgaggcca tagacttctt gaagaaggtg aaccccgcgc
    18481 agtacaggcc ggagaacggc gcgcagtacc cgcgcacaca gttcggcaac gcgctgttgc
    18541 agatcgcgca actcgtcaag gcgggcgtcg gcctcgaagt cgctttcacg gaggtcggcg
    18601 gttgggacac gcacgtcaac gaaggcggcg cacgcggtca actcgcagcg cgcctgtcgg
    18661 agttcggcaa cggcatcaac gcgctctaca cagacctcgg cgcggagcgc atggatgatg
    18721 tcgtgatcct cacgatgtcg gagttcgggc ggacggtgag agagaacggc aatcgcggca
    18781 ccgatcacgg ccacgccaac gccatgttca tcatcggcgg cgcgacgcgc ggcgggcgcg
    18841 tctacggcga ctggccggga ctcgacgcga gcaaacttca cgacgggcgc gacctcgccc
    18901 tgacgacaga cttccgcgac gtgttcggcg aagtcgcaca gagacatctc ggcaacgccg
    18961 acttgaatag agtctttccc ggttacgctt ccagcccggc gaactttcgc gggctgctcg
    19021 gctgagagaa gtaaggatga agtaggaacg atgactaaac cactatgcac taaaagtgca
    19081 tagatttccg caggactgaa agtccgcctc attccaagat gaatgcgtcg ctgtcctgat
    19141 tagattgttg ccgatgatgc tcaagatatt cctgcaccac cgtttcgctg acgtttcctg
    19201 tgctccatgc gcc
//
LOCUS       DASWNS010000054        18804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_131689, whole genome shotgun sequence.
ACCESSION   DASWNS010000054 DASWNS010000000
VERSION     DASWNS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18804
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131689"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..986)
                     /locus_tag="VGB76_01915"
     CDS             complement(216..986)
                     /locus_tag="VGB76_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEY0097685.1"
                     /translation="MIGDVVAKPGRVAVLERIQDLREQHRIDLAVMNAENVAGGFSIT
                     PTLADQLFQSGIDVMTSGNHIFDKRDIIPYIERQGRLLRPANYPPGTPGAGLWVGEVR
                     GVRVAVLNLIGRVFMGTADDPFRVADELVKSIPADVRVRLVDMHAEATSEKSAMGWFL
                     DGRVSAVVGTHTHVQTADECILPQGTAYLSDIGMTGSYAGVIGMDKDDVIARFTSVTQ
                     RRAEHSTGNARICAAVIDVDHETGRAREISRLSLPHES"
     gene            complement(1115..1304)
                     /gene="ssrS"
                     /locus_tag="VGB76_01920"
     ncRNA           complement(1115..1304)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGB76_01920"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1418..1786)
                     /locus_tag="VGB76_01925"
     CDS             complement(1418..1786)
                     /locus_tag="VGB76_01925"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY0097686.1"
                     /translation="MSAIKSPGDSPGTPTIRVEIYNQIYNIRSDGDSEYVTQLAEFVD
                     RRMREISSGTLTVDSLKVAILAALHIADELHRLRRLHEQADSQLAARSGECADLLDRL
                     LKARTVAEQQTTSQSNISFT"
     gene            complement(1807..2088)
                     /locus_tag="VGB76_01930"
     CDS             complement(1807..2088)
                     /locus_tag="VGB76_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097687.1"
                     /translation="MVGLTGLEKFSHLEDKIYRTIELTKTLRQEKEALERDVAATRRE
                     LGSLLDEKDRLETQVERLLSERDMIRMKVEAMLDAIAVLDPEMAEAVRK"
     gene            complement(2262..4412)
                     /locus_tag="VGB76_01935"
     CDS             complement(2262..4412)
                     /locus_tag="VGB76_01935"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY0097688.1"
                     /translation="MIISYNWLRELTGTSLTAGELRERLTMAGLEVEAVHEAAGGDFV
                     LEIAGLSNRPDLLSHLGVAREVCTLTGGALLWPNMQPGREEGRTESFTSVEVQAVDLC
                     PRFTARVVRGVRIAPSPAWLVERLQAVGQRSINNVADITNYVLLELGQPLHAFDLEKL
                     NERRLVVRRAHAGERLTTLDDVERELDAEMLVIADALRPVAIAGVMGGADTEISETTQ
                     DVLIEAAHFAPASVRRTSRALGLQTEASDRFERGVDYDGQLRAQARTVALITELAGGT
                     ATGDAIDLYSQPPPPVVVPLRFSRVKELTGLDVPPQDSIRILGALGIEPLEDAGASRP
                     AETSSESAGQGAGETSVERADFVSPSWRTDIRIEEDLVEEVGRHFGYDKVSDTLPAAN
                     VAGEYRAHEGRRRAARRALNACGFDEALTFSFINAEHDGQFELLPGLVRRADEASQFV
                     TLSNPILEGASRMRATLLAGLLESVRHNFNHGTRDVRLYEMGRVFAANAEMGGQPHET
                     EACALVATGVATEEGHAGAARELDFYDLKGALEAATDGMRVASLEFAAATNAKHLREG
                     QAAQILLAGRPVGTLGRLADEVAAAYKFKQAVYVAEVDLTAMLASEESGVRYAPLARF
                     PSVMRDLSLVADRRVSFAELRRAVVELNLEQVRRVMLVYVYEGERVPEGQRSVTLRLE
                     YRADDRTLRDEEVDELHRRIVNTLEERFGAQLKR"
     gene            complement(4522..5625)
                     /gene="pheS"
                     /locus_tag="VGB76_01940"
     CDS             complement(4522..5625)
                     /gene="pheS"
                     /locus_tag="VGB76_01940"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY0097689.1"
                     /translation="MSSEVREQPQIEAVREAFEQEFGRFERFRGADVAASIAELKSSD
                     AETLLREVADLRTRHTGKKSALANLKKMIGRVAPEERAAFGQQAQQLEAGISGKLEEV
                     ESLLKNYVARLRVERERIDVTLPGRRPRRGHQHPITIVRQRIEDIFVAMGYTVEDGPE
                     IETDFYNFDALNIPAAHPARSPQDTFYTTEGYALRSQTSTVQIHAMQRRKPPLRVIAP
                     GKVFRRDTPDATHNPMFFQLEGLLVDRGITMGHLKGTVTEFLRHLYGPQTRTRFRPSY
                     FPFTEPSAEFDFSCFKCNGAGCRLCKGSGWIELGGSGMVHPNVLRAVGIDPQEFSGFA
                     FGFGIDRMCMLNFGLDDIRLLFENDMRFLEQFN"
     gene            complement(5726..6109)
                     /gene="rplT"
                     /locus_tag="VGB76_01945"
     CDS             complement(5726..6109)
                     /gene="rplT"
                     /locus_tag="VGB76_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY0097690.1"
                     /translation="MPRAKRGNKRTEKRKKILKLAKGYRGNKSKLYRYASEAVDRALN
                     FAYTGRKLKKRDFRSLWIVRIGAAARLNGLNYSQFMHGLKVAGIELDRKVLADLAANS
                     PEAFAELAGQAKNAIESNQQQQAAA"
     gene            complement(6233..6436)
                     /gene="rpmI"
                     /locus_tag="VGB76_01950"
     CDS             complement(6233..6436)
                     /gene="rpmI"
                     /locus_tag="VGB76_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY0097691.1"
                     /translation="MPKLKTHKGAAKRVRTTASGKFKRGHSHARHILTSKTSKRKRHL
                     DIDTLVSEADEQRFKDMLPYGRS"
     gene            complement(6476..7285)
                     /gene="infC"
                     /locus_tag="VGB76_01955"
     CDS             complement(6476..7285)
                     /gene="infC"
                     /locus_tag="VGB76_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY0097692.1"
                     /translation="MNHREARERKCPYGEERHTVAVFKVITGRGRKRLASALNSGGGA
                     ISPSFRGRGQRPDTRRTPSARINDRIRVPEVRVIGDDGEQLGILNTRDAIRMARDRGL
                     DLVEVAATADPPVCRIIDFGKFQYENSKKQKEAKKKQVTITVKEVKFRPGTDDHDYNF
                     KMKHARDWLTDGDKVKATIFFRGREITHRELGAELLQRLEKDLADYGEVEARPRMEGN
                     QMFFIFTPKKQKSAGGGGGGGAKPDGGAAKPATQTLPIATETKTNGGDAPA"
     gene            complement(7210..9261)
                     /gene="thrS"
                     /locus_tag="VGB76_01960"
     CDS             complement(7210..9261)
                     /gene="thrS"
                     /locus_tag="VGB76_01960"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY0097693.1"
                     /translation="MSEVNVQLKEGSQALQLPAGVSVGEALKRLDRDAAKLALAAKVD
                     GREVDLAYKLERAGANGDDSTATVEIEPVLPATRDGLDVLRHSTAHLLAAAVLDLFPG
                     TQLGIGPALLDDPRYGFFYDVIAPRQLTDEDLPVIEKRMRDIAKRNLAYRREEIPKAD
                     ALRIFEERGEPLKCQLVAEKGGETVSVYYIDNSPFIDFCLGPHVPNTNKLKAFKLLTL
                     SGAYWMGDAENAQMQRIYGTAFNTPEELEAWQKQREEAERRDHRRLGRELDLFSIQDS
                     YGQGLIFWHPKGAMIRKKMEDYLYEELIKRNYSFVYTPHIAKRELWQVSGHDQNYGDS
                     MFAPTKLEDTEFRLKPMNCPFHIGIYKSQQRSYRELPQRYAEMGTVYRAELSGTLHGL
                     MRVRGFTVDDAHLFCTPEQVRKEIGDCVDFVIEVFHTFGFEKVKFELSVKGNETNKNY
                     LGAEEDWAQAEGALADALHARGVPYERIEGEAAFYGPKIDFKIEDAIGRLWQLGTVQW
                     DFNLPERFELEYIGEDNKPHRPVMVHRALFGSIERFFGVLIEHYAGAFPLWLAPLQVS
                     VLPITDRINEYAEEVARELRAAGLRVEVNTRSEKIGAKIRDAQLQKVPLMLVLGDREM
                     EQQNVAVRERVQGDLGSMSTEEFKGWARELVEKRELTIGKQESGSVHTGKNDTPSQSS
                     R"
     gene            complement(9608..9682)
                     /locus_tag="VGB76_01965"
     tRNA            complement(9608..9682)
                     /locus_tag="VGB76_01965"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9648..9650),aa:Val,seq:gac)
     gene            complement(10460..11011)
                     /locus_tag="VGB76_01970"
     CDS             complement(10460..11011)
                     /locus_tag="VGB76_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097694.1"
                     /translation="MSDRLFKKTELIANIAIIVVAILLSGVLVKRYIFSGNEAASAPA
                     VDRRIPAGTKAALPGVDWAKNGETLVLVLSRDCRYCTQSAPFYQRLTRAAAGRPDLHL
                     LAVLPQEVEVGKSYLNELGVSVNDVRQASPSTTGAAVTPTLILVDDKGIVKNSWIGQL
                     ATPEENEVLSQLSLERASNDLLN"
     gene            11285..11473
                     /locus_tag="VGB76_01975"
     CDS             11285..11473
                     /locus_tag="VGB76_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097695.1"
                     /translation="MKRKRLLLADLTDAVIPAACDAWGSPGLGDEFRAPRNNGTDLQA
                     TQVDDWQFEFEVAVKPVR"
     gene            complement(11476..13461)
                     /locus_tag="VGB76_01980"
     CDS             complement(11476..13461)
                     /locus_tag="VGB76_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY0097696.1"
                     /translation="MLFRFSEVYKSYGSQDVLRGVSLQINPGEHVGLVGRNGAGKTTL
                     FRLIGGEETPDSGDVVRARGLKLGLLEQHVDFEPGMTVHESALAAFGRLQQIEHEMHE
                     LEHRMGEATDDLDAVLERYSDLQHEFEREGGFEYAARAEAILLGLGFEREMWQLETEK
                     LSGGQKNRLGMARLLLAEPDVLLLDEPTNHLDVAGVEWLEEYLSSYGSAYVIISHDRY
                     FLDRSCRRIIEVENGKAVSYHGNYSAFLVEREERREAQRRAYENQQHLIAKTEEFIRR
                     NLAGQKTKQAKSRRTMLQKMDRVEAVRADGSQGNFQLKGVERTGNQTLIVKELAVGYP
                     DKVLATDITFTLTRGEALGVIGPNGSGKTTFIKTLLGKLPPLAGEARWGTKVQIGYYA
                     QQLDDLDERNEIIMELRRVAPSTVTAGELRGFLAKFLFVGDDVYKHVRDLSGGEKGRL
                     ALAKLIYSRVNVLVLDEPTNHLDIPSRESLEEALDAYEGTIITISHDRYFLDRVATQI
                     LALDGQSAAEHYDGDYTEYHDWKALTRAEAAANARAAQEKQHSAPAAVKTPARAAAQE
                     ESANASRKAKSSEGKGGRKKSSAGAATARTPQLIESEIAAVEKRLAFLSGELSHPEVA
                     RDAARVATLNDEYQQQDAHLRALYEEWERVAVDASSV"
     gene            complement(13637..14848)
                     /locus_tag="VGB76_01985"
     CDS             complement(13637..14848)
                     /locus_tag="VGB76_01985"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY0097697.1"
                     /translation="MYRAAKQISLLTLVALLASVFVACDRSKAVGDNKGTAVAASVND
                     KKIMLTEVDKILEQQAKSQQVQFSDMSQLELAAGRLQVLDELIRREVLFQRAEKESLM
                     PKDDEVAQAINEQKLQLGTQEAFQRALKESGQTEEGMRDIARRQLAIQKLLDRTVSKI
                     EPPTDKEVEDFYNNNKARYVSARGVGLAAIIVDPADNGAANDAKGDIEAKNKIDIINQ
                     RLKSGADFATVAREQSEDPQSVPRGGDLGFFTEDQLKQSGFPQEMISRFLGPMEVGKI
                     TDPFQSNGRWTIFKLTDRRLQNENLTLENPEVRKDATESILNQRKQILNAAMLEVAMR
                     EAKITNNLAQDMLNSPTSLSSLRPAGSRPAQTATPPAATSSPTASSSPATAASPAGGT
                     TSNTNAANAGK"
     gene            15049..15264
                     /gene="rpmB"
                     /locus_tag="VGB76_01990"
     CDS             15049..15264
                     /gene="rpmB"
                     /locus_tag="VGB76_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY0097698.1"
                     /translation="MAKRCEVCGKGPQFGNNVSHANNRTRRRFNPNLQEIRTQRPQGG
                     VVRMKVCTSCIKGNKVTKVAQTAKAAA"
     gene            complement(15333..15737)
                     /locus_tag="VGB76_01995"
     CDS             complement(15333..15737)
                     /locus_tag="VGB76_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0097699.1"
                     /translation="MHTVREIVATEAAPQAIGPYSQAVRIGNFVFTSGQIPIDPATGV
                     FVEGGIAEQTEQVLRNLAEVLRAAGTSLEAVVKTTVFLADMDDFAAMNEVYARYFSNE
                     PPARSTVQAARLPRDARVEIDVIAVRKNNDED"
     gene            complement(15998..18190)
                     /locus_tag="VGB76_02000"
     CDS             complement(15998..18190)
                     /locus_tag="VGB76_02000"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY0097700.1"
                     /translation="MLMIRIEEIVEKVGRNHPQADLDMLRRAYFFSAREHKGQTRASG
                     EPYLVHPLEVANILADMRLDEVSVATGLLHDVVEDTLVEPATIREYFGEEVAHLVEGL
                     TKIAQISNQSKEVQQAENVRKMLLAMIDDVRVVLVKLADRLHNMRTLHYLKPEKRQRI
                     AQETLDIFAPIAHRLGMGKLRSELEDLAFQHLHPEDYRELTAQLEQRRAEHEVFLHAT
                     TARIEEKLREADVPYIRVEGRVKRLYSIWKKLKRQKIELDQVYDLVAARVVLPNEVRH
                     CYAALGIIHNLWKPLPGRFKDWIATPRENLYQSLHTSVIGTEGQPFEVQIRTDEMHRI
                     AEEGVAAHWKYKEGKRGAHEDDEAFQWLRSLIEWTQEVKDSRDFLESLKLDLYPRDVY
                     AFTPMGKIIQLPRGATPVDFAYMIHTEVGNTCTGARVNGRIVPLRTPIQNGDVVEIIR
                     SPNSNPSRDWLNFVVTSKARTSVRHWVSEQQRTESIEIGRKLFEKEAARFQLSTKKLL
                     TDSNGDFKKIAGEYGYGRVDDLLAAIGYGKLVPKNVISKYLGPEKFAELDERKESRLR
                     TSVQAVKRLIRLGDDAIVVRGIDDLLVARARCCNPVHGEPIIGYVTRGKGVAVHAERC
                     KNVQALMVNPERIVAVEWAGRSEHAAYAVKLLAVTENRTGMIAGITNAISDLKTGIRD
                     ARATVASDNHGRIEVTVEVFDVKHLDKVIRAVRGVPGVLDVERLQGAA"
     gene            complement(18400..>18804)
                     /locus_tag="VGB76_02005"
     CDS             complement(18400..>18804)
                     /locus_tag="VGB76_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097701.1"
                     /translation="TLMASKEWNCSYTITEQKENKQTVMKDGKAIIQFKKPKEQADFD
                     KAMKCVNDGMALAEKAISLDPNSEQAWSYKTNLLLEHVKLAEMEGNNDKKAEYQKQAA
                     AAQQKTSQLSEENKRRKDEEEKAKAAKSPASS"
BASE COUNT         3546 a         5662 c         5483 g         4113 t
ORIGIN      
        1 tgcgcctgcg gggcattaac cacgggcgcg gcggaatgct gcgcttgcga agaacaggcg
       61 caacaaagca acaacaataa gggcaacagg gttcgagtct tcatctaaat cctccaggtc
      121 taaacggtgt ggtctgatct atgtgtcggc gtaaactttg ccgccggtga gaaccgtggc
      181 cgggttcgta tcagagcgag agacaaaaag ccgcctcatg attcatgcgg cagagagagc
      241 cgggaaattt cgcgcgcgcg tccggtttcg tgatcaacat ctatgacggc ggcgcagatg
      301 cgcgcgttgc ccgtggagtg ttccgcgcga cgctgtgtga cggaagtgaa gcgcgcgatc
      361 acgtcgtctt tgtccatgcc gatcacgccc gcgtagcttc ccgtcatgcc gatgtccgag
      421 agataagccg tgccttgcgg caggatgcac tcgtccgccg tctggacgtg cgtgtgcgtg
      481 ccgactacag ccgagacgcg cccgtccaga aaccagccca tcgcgctctt ttcagaagtc
      541 gcttcggcgt gcatgtccac gaggcgcacg cgcacgtccg cagggatcga cttgaccaac
      601 tcgtctgcga cgcgaaacgg atcgtccgcc gtgcccataa agacgcgacc gatgaggttc
      661 aggacggcga cgcggacgcc gcgcacctct ccgacccaca agcctgcgcc gggcgtgccg
      721 ggcgggtagt tggcgggacg aagcagacgc ccctggcgtt cgatgtaggg gatgatgtcg
      781 cgcttgtcga agatgtgatt gccggaggtc atcacgtcga tgccgctctg gaagagttga
      841 tctgcgagcg tgggcgtgat ggagaagccg cccgccacgt tctcggcgtt catcacggcg
      901 aggtctatgc ggtgctgctc gcgcaagtcc tgtatgcgct ccaagaccgc cacccgcccc
      961 ggcttcgcca ccacatcccc gatcatcaaa actctcatct agacccctaa aagaaagaga
     1021 cgagcgacga gtgacacgtg gcaagtcaga aaagcaggct gcttcaactt gtcacttgtc
     1081 acctgtcact cgtcacttaa aaaagaggca gaggggcatc cgcaaagccg ttccgaagcc
     1141 gtaattgaac ctgcactcaa caggtgggtg cccttttatg agccgcagcc tttggcagtt
     1201 ccttaacaat gaaggaatgc actggctatc agagcggcgt cgggctccct tatcaacggc
     1261 attggctcaa catgggaaat cggtgacgaa ccacgcagaa ttgattccct cagccaaacg
     1321 ggatactaac ataaatcggc ggcctcaagt caaaagagca aaggcgaaaa agcggctctt
     1381 gaaacttgac aattcgacgc tctttttctt tccgatttta tgtaaaagag atgttgctct
     1441 gactcgtggt ttgctgttcg gcgacggtgc gcgccttgag caaacggtcg agcaggtctg
     1501 cgcattctcc gctgcgcgcg gcgagttggg agtcggcctg ttcgtgcaga cgtctgaggc
     1561 ggtggagttc gtctgcgatg tggagagcgg ccagaatcgc caccttgagc gaatcgacgg
     1621 tgagcgtgcc cgaagagatt tcgcgcatcc gccggtcgac aaactctgcg agttgcgtga
     1681 cgtattcgct gtcgccgtcg gaacggatgt tataaatctg gttgtagatt tccacgcgga
     1741 tggtgggcgt gccgggcgaa tcgccggggc ttttgatggc actcataact taaacgtctc
     1801 cgccgctcat tttctgacgg cttcggccat ctctgggtcg agcacggcga tggcgtcgag
     1861 catcgcttca accttcatgc gaatcatatc gcgctcggac aagagccgct caacctgtgt
     1921 ttcgagccga tctttctcgt cgagcaggct gcccaattcg cgccgcgtcg ccgcgacatc
     1981 gcgttccaac gcttccttct cctgacgcaa cgtcttcgtc agttcgatgg tgcggtaaat
     2041 cttatcctct aagtgtgaaa atttctccag cccggttaat ccgaccatcg cctctccttc
     2101 accataaaac agcaattcaa aattgccgcc cgattatgcg acttcgcgtc tccgagcgca
     2161 agaggtaaaa gtcatagggt cggaggccgg aggtgggagg ttagtggaaa tcaatttttg
     2221 atcttaactc acctctcacc tccggcctct cgcctctgct tttatcgttt caactgtgcg
     2281 ccgaatctct cttccagagt gttgacgatg cgtctatgca gttcgtcaac ctcttcgtcg
     2341 cgcagcgtgc gatcatctgc gcggtattcg aggcggagcg tcacggagcg ttgtccttcg
     2401 ggcacgcgct cgccctcgta gacgtagacg agcatcacgc gccgcacctg ttccagattc
     2461 aactcgacga ccgcgcgccg caactccgcg aaactcacgc ggcgatctgc gacgagcgac
     2521 agatcacgca tgaccgaggg gaaacgcgcg agcggcgcat agcgcacgcc cgactcttcc
     2581 gaggcgagca tcgccgtgag atcaacttcc gccacgtaga cggcctgttt gaatttatag
     2641 gcggcagcca cttcgtccgc gagcctgccg agcgtgccga ccggacgccc tgccagcaga
     2701 atttgcgccg cctgtccttc gcgcaggtgc ttcgcgttcg tcgccgccgc gaactccaga
     2761 gaggcgacgc gcattccgtc cgtcgccgct tcgagcgcgc ctttgaggtc gtagaaatcg
     2821 agttcgcgcg ctgcgcccgc gtgcccttct tcggtcgcca cgcctgttgc gacgagcgcg
     2881 cacgcttccg tctcgtgcgg ttgcccgccc atctccgcgt tcgccgcgaa gacgcgcccc
     2941 atctcgtaga ggcgcacgtc gcgcgtgccg tgattgaagt tatggcgcac cgattcgagc
     3001 agaccggcaa ggagtgtcgc cctcatgcgc gacgcgcctt cgaggatcgg attgctgagc
     3061 gtgacaaact gcgacgcctc atccgcgcgc cgcacgagtc cgggcagcaa ctcgaactgc
     3121 ccgtcgtgtt ccgcattgat gaagctgaag gtcaaagcct cgtcgaaccc gcaggcgttc
     3181 aaagcgcgac gcgccgcgcg cctgcgcccc tcgtgcgcgc gatattcgcc cgcgacgttt
     3241 gcggcgggca gcgtgtccga aactttgtcg taaccgaaat gacgcccgac ttcttcaact
     3301 aaatcctctt cgatgcgtat gtccgtgcgc catgacgggc tgacaaagtc tgcgcgctcg
     3361 acgctcgttt cgcccgcgcc ctgccctgcg ctttcgcttg aagtttcagc cggacggctc
     3421 gcgcccgcgt cctccaacgg ctcgatgccg agcgcgccga gtatgcgaat cgaatcctgc
     3481 ggcggcacgt ccagacccgt caattctttg acgcgcgaga agcgcaaggg aacgaccacc
     3541 ggcggcggcg gttgcgagta aaggtctatt gcatcgccgg tcgccgtgcc gcccgcgagt
     3601 tcggtgatga gcgcgacggt acgcgcctgt gcgcgcaact gcccgtcgta atctacgccg
     3661 cgctcgaagc gatccgacgc ttcggtctgc aaacccagag cgcgtgaagt gcgcctgacg
     3721 gacgcgggcg cgaagtgcgc cgcttcaatc aaaacgtcct gcgtcgtctc ggagatttcg
     3781 gtgtccgcgc cgcccatcac gcccgcgatg gctacgggtc gcaaggcatc ggcgatgacc
     3841 agcatctccg catcgagttc gcgttcgaca tcatcgagcg tggtgagccg ttcgcccgcg
     3901 tgcgcgcgac ggacgacgag acgccgctcg ttcaacttct ccagatcgaa tgcgtgaagc
     3961 ggctgaccca gttcgagcag cacgtagttg gtgatgtccg cgacgttgtt gatcgaacgc
     4021 tgcccgaccg cctgcaaacg ctcgacgagc cacgcgggcg acggcgcgat ccgcactccg
     4081 cgcacgacgc gcgcagtgaa acgcgggcat agatcaacgg cctgcacttc gaccgaagta
     4141 aagctctccg tgcgcccttc ctcgcgcccc ggctgcatgt tcggccacag cagcgcgccg
     4201 cccgttaaag tgcagacctc gcgcgcgacg cccagatgcg acagcaggtc aggacgattc
     4261 gacagccccg cgatctccaa cacgaaatcg ccgcccgccg cctcgtgtac cgcttcgacc
     4321 tcaagccccg ccatcgttaa acgctcgcgc agttcgcccg ccgtcaggct tgtgcccgtc
     4381 aattcgcgta gccagttgta gctaataatc ataattcagt gacaagtgac gagtgacgag
     4441 tgacgagtga cgagccgaag acaagtttaa agacaggaat gctgttttaa cttgtcaccc
     4501 gtcgcccgtc actcgtcact gttagttaaa ctgttcgagg aagcgcatgt cgttctcgaa
     4561 gaggaggcgg atgtcgtcga ggccgaagtt cagcatgcac atgcggtcga tgccgaagcc
     4621 gaaggcgaag ccggagaact cctgcgggtc gatcccgact gcgcgcagga cgtttggatg
     4681 caccatcccc gaaccgccga gttcgatcca gcccgaacct ttgcagagac gacagcccgc
     4741 gccgttgcac ttgaagcaac tgaagtcgaa ttccgcgctc ggctccgtga acggaaagta
     4801 agaggggcga aaacgcgtgc gcgtctgcgg cccgtagagg tggcgcagga actccgtaac
     4861 cgtgcccttc aagtgtccca tcgtgatgcc gcgatcaacc aaaagccctt cgagttggaa
     4921 gaacatcggg ttgtgcgtag catcgggcgt gtcgcggcga aagactttgc cgggcgcgat
     4981 gacacgcaga gggggcttgc gccgctgcat cgcgtgaatc tgcacggtcg aagtttgcga
     5041 gcgcagggcg tagccttccg tagtgtagaa agtgtcctgc gggctgcgcg cggggtgcgc
     5101 cgccggaatg ttgagcgcgt cgaagttgta aaaatccgtc tcaatctcgg ggccgtcttc
     5161 aaccgtgtag cccatcgcca cgaagatgtc ttcgatgcgc tggcggacaa tcgtgatcgg
     5221 atgctggtgt ccgcgacgcg ggcgacgacc gggcagcgtc acgtctatgc gctcgcgctc
     5281 gacgcgcaaa cgcgcgacgt agtttttcaa caagctctcg acttcttcaa gctttccgct
     5341 tatccctgct tcaagctgct gcgcctgttg cccgaacgcc gcgcgctcct ccggcgcgac
     5401 gcgcccgatc atcttcttga gattggcaag cgcactcttc ttgcccgtat ggcgcgtgcg
     5461 cagatcagca acctctcgca ggagagtttc ggcatcactc gatttcaact cggctatgct
     5521 tgccgccaca tctgccccgc gaaaacgttc gaagcgtccg aactcttgct cgaacgcttc
     5581 gcgcaccgct tcgatttgcg gctgttctct gacttccgaa ctcattccgg aacctcaatc
     5641 tcgtgaatcg agaatcataa attgtaaata agaggagagc agtttcccga tctattcacg
     5701 atttacgatt cacgattcac ggcttttagg ccgctgcctg ttgctgctgg ttgctctcaa
     5761 tcgcgttctt cgcctgtccg gccagttccg caaacgcttc cggcgagttg gcggccaggt
     5821 cggccagaac tttacggtcg agttcgatgc ctgcgacctt caagccgtgc atgaactgcg
     5881 aatagttcaa gccgttcaga cgcgccgccg cgccgatgcg cacgatccag agactacgga
     5941 agtcgcgctt cttcaacttg cgacccgtgt aagcgaaatt cagcgcgcgg tctaccgcct
     6001 cactggcgta gcgataaagt ttcgacttgt taccgcgata gcccttcgct aatttaagta
     6061 tcttcttgcg cttttccgtg cgcttatttc cacgttttgc cctcggcata acattctcct
     6121 cctaataatt cagtgacgag tgacgagtga cgaacgacga gtgagagaca agttcaagac
     6181 agggtttctg ctgttttaac ttgtcactcg tcacttgcca ctcgtcactc tcctagcttc
     6241 tcccgtaggg cagcatgtct ttgaagcgct gctcgtcggc ctcggacacg agcgtgtcga
     6301 tgtcgagatg tcgtttacgt ttcgacgttt tgctcgtcag gatgtggcgc gcgtgcgaat
     6361 ggccgcgctt gaacttgcct gacgccgtag tgcggacgcg tttggccgcg cccttgtgtg
     6421 ttttgagttt tggcataata acaacccctt ctttctgcta caaaaatatc tctgctcaag
     6481 ccggtgcatc gccgccgttg gttttcgtct cggtcgcgat gggcaaagtt tgcgtcgccg
     6541 gttttgctgc gccgccgtcc ggcttcgcac cgccgccacc tccgccgccc gcgctcttct
     6601 gcttcttggg cgtgaagatg aagaacatct gattgccctc catgcgcggg cgcgcctcca
     6661 cttcgccgta gtcggccaaa tctttctcca gacgctgcaa caactccgcg ccgagttcgc
     6721 ggtgcgtgat ctcgcgcccg cgaaagaaga tcgtggcttt aactttgtcg ccgtccgtca
     6781 gccaatcgcg cgcgtgcttc atcttgaagt tgtagtcgtg atcgtccgtg cccggtcgga
     6841 acttcacttc cttgaccgtg atcgtgacct gcttcttctt cgcctctttc tgcttcttgc
     6901 tgttctcgta ctggaacttg ccgaagtcga tgatgcgaca gacgggcggg tcggcggtcg
     6961 ccgccacttc cacgagatcg aggccgcgat cacgcgccat gcgaatagca tcgcgcgtgt
     7021 tcaagatgcc gagttgctcg ccgtcgtcgc cgatgacgcg cacttcaggc acgcgaatcc
     7081 ggtcgttgat gcgcgcagag ggtgtccggc gtgtgtcagg acgctgtccg cgcccgcgaa
     7141 aacttggtga gattgctcca cctcctgagt taagggctga tgcgagccgc ttccgcccgc
     7201 gcccggttat taccttgaag actgcgacgg tgtgtcgttc ttccccgtat ggacacttcc
     7261 gctctcttgc ttcccgatgg ttaattcgcg tttttccacc aactcacgcg cccatccctt
     7321 gaactcttcc gtcgacatcg aaccgaggtc gccctgcacg cgctcgcgca cggcgacatt
     7381 ttgttgctcc atctcgcggt cgccgagcac gagcatgagc ggcacttttt gcaactgcgc
     7441 gtcgcgtatc ttcgcgccga tcttctcgct cctcgtattc acctcgaccc gcaagcccgc
     7501 cgcgcgcaac tcacgcgcaa cctcttccgc atactcgttg atgcgatccg tgatcggcag
     7561 caccgacacc tgcaagggcg cgagccagag cggaaacgcg cccgcgtagt gttcgatgag
     7621 gacgccgaag aagcgttcaa tcgaaccgaa gagcgcgcga tgcaccatca cggggcgatg
     7681 cggcttgttg tcctcgccga tgtactccag ttcgaagcgt tccggcaagt tgaaatccca
     7741 ctgcaccgtg ccgagttgcc agagccgccc gatggcgtct tcgatcttga agtcaatctt
     7801 cggaccatag aaagcggcct cgccttcgat gcgctcgtaa gggacgcccc gcgcgtgcaa
     7861 agcatccgcg agcgcgccct cggcctgcgc ccagtcctct tccgcgccga gataattctt
     7921 gttcgtctcg ttgcccttga ccgacaactc gaacttcact ttttcgaaac cgaacgtgtg
     7981 gaaaacttcg ataacgaagt ccacgcaatc gccgatctct ttgcgcacct gttcgggcgt
     8041 gcagaagagg tgcgcgtcgt ccaccgtgaa gccgcgcacg cgcatcaggc cgtggagcgt
     8101 ccccgacagt tccgcgcgat agaccgtgcc catctccgcg taacgttgcg gcagttcgcg
     8161 gtaagagcgt tgctgcgact tatagatgcc gatgtgaaac gggcagttca taggcttcag
     8221 gcgaaactcc gtatcttcga gtttcgtcgg cgcaaacatc gaatcgccgt agttctggtc
     8281 gtgcccgctc acttgccaca actcgcgctt cgcaatgtgc ggcgtataga cgaaactgta
     8341 gttgcgcttg atcaactctt cgtacaggta atcttccatc ttcttccgga tcatcgcgcc
     8401 cttcggatgc cagaagatca agccttgccc gtaactgtcc tgaatcgaga agagatcgag
     8461 ttcgcgcccc aggcggcgat ggtcgcgtcg ctcggcttct tcgcgctgct tctgccacgc
     8521 ttcgagttct tccggcgtgt tgaaggccgt gccgtagatg cgttgcatct gcgcgttttc
     8581 ggcgtcgccc atccagtatg cgccggagag cgtgagcagt ttgaaggctt tcagtttatt
     8641 cgtgttcggc acgtgcgggc cgagacagaa atctatgaac ggcgaattgt ctatgtaata
     8701 gacgctcacc gtctcgccgc ctttctccgc caccaactgg cacttgagag gctcgccgcg
     8761 ctcctcgaag atgcgcagcg cgtctgcctt cggaatctct tcgcgccggt acgcgagatt
     8821 gcgtttggcg atgtcgcgca tgcgcttttc gatcacgggc agatcttcgt ccgtcaactg
     8881 tcgcggggcg atcacgtcgt agaagaaacc gtaacgcggg tcgtcgagga gcgcgggacc
     8941 aatgccgagt tgggtgccgg gaaagagatc gagaacggcg gcggccaaaa gatgcgccgt
     9001 cgaatggcgc agcacgtcga gtccgtcgcg cgtggcgggc aacaccggct cgatctcgac
     9061 ggtcgccgtg ctatcgtcgc cgttcgcgcc cgcccgctcc aacttgtacg cgaggtcaac
     9121 ttcgcggcca tccactttcg ccgcgagcgc gagtttcgcc gcgtcgcggt cgagtcgctt
     9181 caaagcctcg cccacgctca cgcccgcagg caattgcaaa gcctgcgagc cttctttcag
     9241 ttgtacgttt acttcactca tcgcctgtca tctccgcgaa agatttaaag agactctcgg
     9301 cgcgcatcgc gcatataagc gacaacacgc gacgcgccct cgcgtcacat gggttggaag
     9361 aaaaaccctc ctgacacgag cggcggcctt cgcggaagca aaagcgtctg acctgattct
     9421 gaattttcgc gttcgcctaa aggtaaacga acaattacga gatcggattc gacagaagcg
     9481 cctttgccgt gcgacgccgt gacacaacgt gtggcgggtc agctttgatt agaatcgaaa
     9541 cgtagttcga gttcgcatca acataaaagc aaaggtcttc cggagctcac tgtcgaaaaa
     9601 aggaaaatgg taggctcgtg gggatttgaa ccccagaccc ctaccgtgtc aaggtagtgc
     9661 tctacccctg agctacgagc ctacaactac aaacaacatc gcttcaaaca cgtctgaaaa
     9721 cgaactcgta aagtaacaaa cgccccgcgc acatgtcaag caaggcgcag cccgctaaaa
     9781 tcgcatgatc aggctcggat attccttcga catcttttcc gctcaagcat tcgcggctcg
     9841 cccttgagat caaatatttg acagccgcga cgcgagtttc cagcggctcc cgggtgcggg
     9901 acggctccgc tcaagtgggc tgggaaaaga cgcgcggggc gaaagctccg tctctgcctg
     9961 ttgctcaatt caacgagcaa taagctggaa cagagcctcc gccccgacga agtttttgac
    10021 gttggacaac accaacgtct cttgcccgcg ccggaaggag actcaggcgc gcggttggcg
    10081 aagcgtccgt ctcctagcag gagaaagagc accaacgcca gcatctccta taaacttcct
    10141 gaacgcacgg ccccgggtct gggagcgtgt agcaatcggc ttcctgctct tcgcaactgc
    10201 tacagcaggg agcggcagcg gtctgcctca cggtgaggtc ggaagacgcg acgatgaaac
    10261 ctgcacacaa gacgacaagc atggcgaact tgcgcaacag tctgagttta tctgtccggt
    10321 tcatgtctgt atcctttcgc tttgaggtct gttgatgagg tggcaacctg tctacagtat
    10381 gcgggaaacc ggcttgccta ccgatttccg ccgaagccgg gcacgcctcg cgtttaagtt
    10441 cggcgcgcga atgagaacgt caattcaaga gatcgttgct cgcacgctcc agcgacagtt
    10501 gactcaagac ttcattctct tcgggagtcg ccagttgccc gatccaagag tttttgacga
    10561 tgcctttgtc atcgacaaga atgagcgtcg gcgtcacggc tgccccggtc gtcgagggcg
    10621 acgcctgcct gacatcgttg acggagacgc ccagttcgtt cagatagctc ttgccaactt
    10681 ccacctcctg cggcaacacg gccagcaaat gcaggtcggg acgccccgcc gccgcgcgcg
    10741 tcagacgttg ataaaaaggg gcgctctgcg tgcagtagcg gcaatctcgc gagagaacca
    10801 acacgagcgt ctcgccgttc ttcgcccagt ccacgccggg caatgcggcc ttcgtgccgg
    10861 cggggattct ccggtcgacg gcgggcgcgg acgcagcttc attgccactg aagatgtatc
    10921 ttttgacgag gacgccgctg agtaatatcg cgaccacgat gatcgcaatg ttggcgatga
    10981 gttcggtctt cttgaagagg cggtctgaca taaatttgtc ctcacatttc gattggaagg
    11041 agagttcaca gacagccccg aacgccgtta ctcgcacacg tccggagaca ctgccgcccg
    11101 ggcgatgcgg ctcgctgtag gaacgggtgc gtatgattta cggcgcgtga gggctaccac
    11161 atgttgttct ctgtaaactg gctttgactg tttagatcag cgagagagta ggggtcgcca
    11221 atccaggatt aacaaaggga ggacgaattt atataccttg ccccgcaggg tgtcaatata
    11281 aagcgtgaag cgaaagagac ttttgcttgc agacctcacc gacgctgtca ttcccgccgc
    11341 ttgcgatgcg tggggcagcc ccggcctcgg cgatgagttt cgcgcgcccc ggaataacgg
    11401 cacggactta caggcgacgc aagttgacga ctggcaattc gaattcgaag tcgctgtaaa
    11461 acccgtccgt taaattcaga cactcgaagc atctaccgcg acgcgctccc attcttcata
    11521 gagcgcgcgg agatgtgcgt cctgctgctg gtactcgtcg ttgagagttg ccacgcgggc
    11581 ggcgtcgcgc gcaacttcgg ggtgggacaa ttctccagag aggaacgcca gacgcttttc
    11641 aaccgccgcg atctcgctct cgatgagttg cggcgtgcgc gccgtcgccg ctcccgccga
    11701 cgactttttc ctgccgccct taccctctga acttttcgct ttgcgcgaag cgttcgcgct
    11761 ctcttcctgc gcggcggcgc gggcgggagt ctttacggcg gcgggcgcgc tgtgctgctt
    11821 ctcctgagcg gcgcgagcgt tggcggcggc ctccgcgcgt gtgagagctt tccaatcgtg
    11881 atactcggtg taatcgccgt cgtaatgttc ggctgcgctc tggccgtcga gcgcgagaat
    11941 ttgcgtcgcc acgcggtcga gaaaataacg gtcgtggctg atggtgatga tcgtgccttc
    12001 gtaagcgtcg agggcttctt ccagactctc gcgcgatggg atgtcgaggt ggttggtcgg
    12061 ctcgtcgagg acgagcacgt tgacgcgcga gtagatcaac ttggcgagcg cgagcctgcc
    12121 cttctcgccc cccgaaagat cgcgtacgtg cttgtaaacg tcgtcgccga cgaagagaaa
    12181 cttggcgagg aaaccgcgca actcgcccgc cgtcacggtt gaaggcgcga cgcgacgcag
    12241 ttccatgatg atctcgttgc gttcgtccag atcgtcgagt tgctgcgcgt agtaaccgat
    12301 ctgaactttc gtgccccaac gcgcctcgcc cgcgagcggt gggagtttgc cgagcagcgt
    12361 cttgatgaag gtcgtcttgc ccgaaccgtt cggcccgatg acgccgaggg cttcgccccg
    12421 cgtgagcgta aacgtgatgt ccgtcgccag caccttatcc ggataaccga cggcgagttc
    12481 tttgacgatc aatgtctggt tgcccgtgcg ctcgacgcct ttcaactgga agttgccctg
    12541 cgacccatcg gcgcgcacag cttcgacgcg atccattttt tgcagcatcg tgcggcgcga
    12601 cttcgcctgc ttggtcttct gtccggcgag attgcggcga ataaattctt cggtcttggc
    12661 gatgaggtgt tgctggtttt cgtaagcgcg ccgctgcgcc tcgcgccgct cttcgcgctc
    12721 gacaaggaag gccgaatagt tgccgtggta actcaccgct ttgccattct ccacttcgat
    12781 gatgcggcga cacgagcggt cgagaaaata acggtcgtgc gaaatgatga cgtaggccga
    12841 cccgtagctc gaaagatact cttcgagcca ttcgaccccg gccacgtcca gatggttcgt
    12901 cggctcgtca agcaggagca catcgggttc ggcgagcagg aggcgcgcca tgccgagacg
    12961 gttcttctgc ccgcccgaaa gtttctccgt ctccagttgc cacatctcgc gctcgaagcc
    13021 gaggccgagc aggatcgctt cggcgcgtgc ggcatactcg aaaccgccct cgcgctcgaa
    13081 ttcgtgttgg aggtcgctgt agcgttcgag caccgcatcg agatcgtcag tcgcttcgcc
    13141 catacggtgt tcgagttcat gcatctcgtg ctcgatctgc tggaggcgac cgaaggccgc
    13201 gagcgcggat tcgtgcacgg tcatgcccgg ctcgaaatcc acgtgctgtt cgagcaggcc
    13261 gagtttgagg ccgcgcgcgc gcaccacgtc gccgctatcg ggcgtctctt cgccgccgat
    13321 caggcgaaac agggtggtct tgcccgcgcc gttgcgcccg acgaggccga cgtgctcgcc
    13381 cggattgatc tggagcgaca cgccgcgcaa cacatcctgc gagccgtaag atttatagac
    13441 ttccgagaaa cgaaacaaca taagaacggt gacgagtgac aggtgacgag ttggtaaatc
    13501 gtaatcggta aatcgtaatc ggtaaatcgt aaatcgtgaa tcgtaaacgg agactgcttt
    13561 ctccgtttac gattcacgat ttgtgtgttc gagccgaacc ggcgatggca gccgcagcag
    13621 ttcaactacg cgcgagctat ttgccggcgt tcgctgcgtt ggtgttggac gttgtgccgc
    13681 ctgccgggct cgccgccgtc gcgggcgaag aggaggccgt ggggcttgaa gtcgcggcgg
    13741 gcggcgtggc cgtctgcgcc ggacgacttc cggctggacg caggctgctc agcgaagtcg
    13801 ggctgttgag catgtcctgc gccagattgt tcgtgatctt cgcctcgcgc atggcaactt
    13861 cgagcatcgc cgcattcagg atttgcttgc gctggttgag aatgctctcc gtcgcatcct
    13921 tacgcacttc cggattttcg agcgtcaaat tctcgttctg caaacgcctg tccgtcagct
    13981 tgaagatcgt ccaacgaccg ttgctctgaa acgggtcggt gattttgccg acttccatgg
    14041 ggccgagaaa tcttgagatc atctcctgcg ggaagccgct ctgcttgagt tgatcttcgg
    14101 tgaagaaacc gaggtcgccg ccgcgcggca cgctctgcgg gtcttcgctc tgttcgcgcg
    14161 cgacggtggc gaaatccgcg ccgctcttga ggcgttggtt gatgatgtcg atcttgttct
    14221 tggcctcgat gtcgcccttg gcatcgttcg ccgcgccgtt gtccgccggg tcgacgatga
    14281 tggcggccag cccgactccg cgcgcgctca cgtaacgcgc tttgttgttg ttataaaaat
    14341 cttcgacttc cttgtcggtc ggcggctcga tcttcgagac ggtgcggtcg agcagttttt
    14401 gaatcgccaa ctggcggcgc gcgatgtcgc gcatgccctc ttcggtctgc cccgattctt
    14461 tgagcgcacg ttggaacgct tcctgcgttc cgagttgcag tttctgctcg ttgatggctt
    14521 gcgcaacctc gtcgtctttg ggcatgaggc tctctttctc ggcgcgctgg aagaggactt
    14581 cgcggcgaat caactcgtcg agaacctgca agcgtccggc ggcaagttcg agttgcgaca
    14641 tgtcggaaaa ctgcacttgc tggctcttgg cctgctgctc cagaatcttg tcaacctcgg
    14701 ttaacatgat ctttttatcg ttgaccgaag cggcgacggc tgtgcccttg ttgtcgccga
    14761 cggccttgct gcggtcgcag gcaacgaaga cagacgcgag taacgcgacg agggtgagaa
    14821 gactgatctg tttggcggct ctatacactg tggatttcta ctccttcaac ttttatgtga
    14881 tgatccaatc gaacttgcgc gcgagcggca aagggcagcc tgcgcgcttg acctctggta
    14941 aaactgtttg ctatatattt tggtttcccg cgtgcttcaa gtcctcactg tgtgagttgg
    15001 caatagacgc ggcgcgcttt ctactattcg gaggtacaca aagcaatcat ggctaaacga
    15061 tgcgaagtct gcggcaaagg cccacaattc ggaaacaacg tctcccacgc gaacaatcgc
    15121 acccgcaggc gtttcaaccc caacctgcaa gagattcgca cgcaacgccc gcaaggcggg
    15181 gtcgtgcgta tgaaggtttg caccagttgc atcaagggca acaaagtaac gaaagtggcg
    15241 caaacggcga aagcagcagc ttaatttaaa cgacgaagta agaacgcgga gcgacgaggt
    15301 gaaagcaaag tctttcattc atcgttccgc gtttaatctt catcgttatt ctttctaact
    15361 gctatcacgt cgatttcaac gcgcgcatcg cgtggcagac gcgcggcctg gacggttgaa
    15421 cgcgcgggcg gttcgtttga aaaatagcgc gcgtaaactt cgttcatcgc cgcgaagtcg
    15481 tccatatcgg cgagaaacac ggtcgtcttc accaccgctt ccagactcgt tcccgccgcc
    15541 cgcaagacct ccgcgagatt gcgcaaaacc tgctcagtct gctccgcgat tccaccttcg
    15601 acaaacacgc ccgttgcagg atcaatcgga atctgccccg acgtaaacac gaagttgcct
    15661 atccgcacgg cctgcgaata aggaccgatg gcttgcggcg cggcctctgt tgcgacgatc
    15721 tccctcacgg tgtgcatatc ctctttttag tctcaccaag caaattgcgc attgtaacaa
    15781 aaactccgcc gctagaccaa cgcgagagca ccgacgagtt gcgggcaggt gcgctcgtcg
    15841 cgtcgaggat taaggcggaa agcagactta aatattacag atgagtttta tggcgtgacg
    15901 gatgcttggc tgtgaattaa taacagaggc gcggcaaact gttgtggcgt cgccgcgtgg
    15961 cggtgcgcgg cagttccggc gtcacgcctg ccgggtgtca ggcggctccc tgcaagcgtt
    16021 ccacgtcgag cacgccgggc acgccgcgca cggcgcggat aactttatcg agatgcttca
    16081 catcgaagac ctcgaccgta acctcgatgc gcccgtgatt gtcggacgca accgttgccc
    16141 gcgcatcgcg gatgccggtc ttcaagtcgg agatcgcatt tgtgattccg gcgatcatgc
    16201 ccgtgcgatt ctccgtgacg gcgagcagtt tgacggcata agccgcgtgc tcgctcctgc
    16261 ccgcccattc aaccgcgacg atacgctccg ggttgaccat caacgcctgc acgttcttgc
    16321 aacgttcggc gtggacggcg actcccttgc cacgcgtgac ataaccgatg atcggctcgc
    16381 catgcaccgg attgcagcaa cgcgcgcggg cgacgagcag atcgtctatg ccgcgcacga
    16441 ctatcgcatc gtcgccgaga cggatgagac gcttgacggc ctgcacgctc gtgcgcaggc
    16501 gcgactcttt gcgctcgtcg agttcggcga atttttcggg gccgagatat ttggagatga
    16561 cgttcttcgg gacgagtttg ccgtaaccga tagcggcgag cagatcgtcc acgcgcccgt
    16621 agccatactc gcccgcgatc tttttaaagt cgccgtttga atccgtgagc agtttcttgg
    16681 tcgagagttg gaagcgcgcg gcctccttct cgaaaagttt gcgaccgatc tcgattgatt
    16741 ccgtgcgttg ctgctccgaa acccagtggc gcacgctggt gcgcgctttt gaagtgacga
    16801 cgaaattgag ccagtcgcgc gaagggtttg agttgggcga gcgaatgatc tcgaccacat
    16861 cgccgttctg aatcggcgtg cgcaagggca cgatgcgtcc gttgacgcgt gcgcccgtac
    16921 aggtgttgcc gacctcggtg tgaatcatgt aggcgaagtc aacgggagtc gcgccgcgcg
    16981 ggagttggat gatcttgccc atcggcgtga aggcgtaaac gtcgcgcggg tagagatcga
    17041 gtttgagcga ttcgagaaaa tcgcgcgagt ctttgacttc ctgcgtccat tcgatgagcg
    17101 agcgcagcca ttggaaggcc tcgtcgtctt cgtgcgcgcc gcgcttccct tccttgtatt
    17161 tccagtgcgc ggcgacgccc tcttccgcga tgcgatgcat ctcgtcggtg cgaatctgca
    17221 cctcaaaagg ctgcccttcc gtgccgatga cggaggtgtg gagcgattgg tagagattct
    17281 cgcgcggcgt ggcgatccaa tctttgaagc gaccgggcag aggcttccat aagttgtgaa
    17341 tgatgccgag cgcggcgtag caatgacgca cctcgttcgg caggacgacg cgcgccgcga
    17401 ccagatcgta aacctgatca agctcgatct tttgtcgctt caatttcttc cagatcgaat
    17461 agagacgctt gacgcgcccc tcgacccgta tgtaaggcac gtcggcctcg cgcagtttct
    17521 cttcgatgcg cgcggtggtg gcatgaagga agacttcatg ctcggcgcgt cgttgttcga
    17581 gttgggctgt gagttcgcgg tagtcctctg ggtgcaggtg ctggaaggcg aggtcttcga
    17641 gttcgctgcg cagtttgccc atgccgaggc ggtgcgcgat gggggcgaag atgtcgagcg
    17701 tctcctgcgc gatgcgttgc cgcttttccg gtttcaggta atgcagcgtg cgcatgttgt
    17761 gcaggcggtc tgcgagtttg accagcacga cgcgcacgtc gtcgatcatc gccagcagca
    17821 tcttgcgcac gttctcggcc tgctgcacct ccttcgactg gttactgatc tgcgcgatct
    17881 tcgtcaagcc ctcgacgaga tgcgccacct cttcgccgaa gtattcgcgg atcgtcgccg
    17941 gttcgacgag cgtgtcttcc accacgtcgt gcaggagtcc ggtggcgacg gaaacttcgt
    18001 cgagccgcat gtctgccaga atgttggcga cttcgagcgg gtgtacgaga taaggttcgc
    18061 cggaggcgcg cgtctgccct ttatgttcgc gcgccgaaaa aaagtaggcg cgacgcaaca
    18121 tatccagatc ggcctgcgga tgattgcgcc caactttttc gacgatctct tcgatccgaa
    18181 tcataagcat tcgtgaatcg tgaatcgtaa atcgtgaaga gaggagttcc ggtttgttct
    18241 attcacgatt tacgattcac gatttactga taagaatagg tgatgcccga ctcgaaagtc
    18301 gagcatcacc tctttttcgg gcagtgaaag tttactgaaa aagcttcgaa cgcgggagac
    18361 tcgttcgtgt cgccctgcgc gcgtaccggc gccagcgagc tagcttgaag cgggcgactt
    18421 ggccgctttg gctttctctt cttcatcctt cctgcgtttg ttctcttcac tcagttgaga
    18481 agtcttctgc tgggcggcgg cggcctgttt ctggtattcg gccttcttgt cgttgttgcc
    18541 ttccatctcc gccagtttga cgtgctccaa cagcaggttt gttttgtaag accaggcctg
    18601 ctcgctgttc gggtcgaggc tgatggcctt ctcggccaac gccatgccgt cgttgacgca
    18661 cttcatcgcc ttgtcgaagt ctgcctgctc cttcggcttc ttgaactgga tgatggcctt
    18721 gccatctttc atcacggtct gcttgttttc cttctgctcc gtgatggtgt aggagcagtt
    18781 ccactccttg ctcgccatca gcgt
//
LOCUS       DASWNS010000055         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_365379, whole genome shotgun sequence.
ACCESSION   DASWNS010000055 DASWNS010000000
VERSION     DASWNS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365379"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VGB76_02010"
     CDS             <1..351
                     /locus_tag="VGB76_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0097702.1"
                     /translation="RLMGLNTGRVTIGVYALSGALAGLAGVLDAARLGAGRPVAGMGD
                     ELNAIAAVVVGGTLLTGGQGSVMSTLVGVLLLGLIFNVFNFEGWISPWWQAVLRGLFL
                     LVVVIVQNRLSRRK"
     gene            564..1706
                     /locus_tag="VGB76_02015"
     CDS             564..1706
                     /locus_tag="VGB76_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097703.1"
                     /translation="MKVSIRVRFFLSLVALLSVMSVAVRAQDARSTLTNFPESQAILY
                     INARRLINEAAPAVVPPAALDKALGEAKQFIDLNGLDYVIAGARLKGAVSLKQLPEVL
                     LVVRGNFSADGLLSAARMAGQGMYVQETYGGKTLNVFTLNKPATTADGSGGGGGEQSG
                     RKFPVDQIAAVALDANTLAIGIPAYLRDAIDAGAGGGTTARLKPELVDLTLRDGNTLV
                     SLVTDVPPAMSQHLRLLGMPPNAETDRLIDALRQVQLSVNMQPGTFGVQSILRMDSSE
                     SASALNGLVGMGATLAKAQIAKEVGKQTSSGKDDMAALLRVLETLTNTARDNEVVLGI
                     TFQQASLAEVIKRQMMPKKQETAITPRPDGNKGAAVKRRRPRTRRR"
     gene            1720..>1986
                     /locus_tag="VGB76_02020"
     CDS             1720..>1986
                     /locus_tag="VGB76_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097704.1"
                     /translation="MNKGRYTEAEHDSFAEAVLRDGFCLLPGHFSKETLEAWRAAFAL
                     LLEEHIEREGHLRNRGAARYYVTLPFIAPFADPAIYEDADVLAVI"
BASE COUNT          389 a          576 c          645 g          376 t
ORIGIN      
        1 cgtttgatgg gcttgaacac ggggcgcgta acgattggcg tctatgcttt gagcggcgcg
       61 ctcgcagggc tggcgggcgt gctcgatgcc gcgcgactcg gcgcggggcg acccgtcgca
      121 gggatgggcg acgagttgaa cgcgattgcg gcggtcgtcg tcggcggcac actcttgacg
      181 ggcgggcagg gcagcgtgat gtcaacgctc gtcggcgtcc tgctgctcgg actcatcttc
      241 aacgtcttca acttcgaggg atggatcagc ccctggtggc aggcagtcct gcgcgggctg
      301 ttcctgctgg tggtcgtcat cgtgcagaac cgcctgagca ggcgaaagtg acgcgaggga
      361 gcggcggcgg aaaccggaat ccaaaatact gaatccggaa tccaaaatta agcgtacagg
      421 aatcagcagt gataatcgct gaattttctt ccggctatac tgcttctgaa ggcagtctaa
      481 ggttctttgg atttcgtcta ccgtgtcgca tcggttaaaa tccgaagagt tctggccgtt
      541 atgtcgaaag gataattgac accatgaaag tctccatccg cgtcaggttc ttcctctcgc
      601 tcgtcgcgct tctctcggtc atgtctgtcg ccgtccgggc gcaggatgcg cgcagcacgc
      661 ttacaaactt tcccgaatca caggccatcc tgtatatcaa cgcgcgccgc ctgattaacg
      721 aggccgcgcc tgccgtcgtg ccgcccgccg cgctcgacaa ggcgctcggc gaggcaaagc
      781 agtttatcga cctcaacggg cttgattatg tgatcgcggg cgcgcgcctc aagggcgcgg
      841 tgtctctgaa acagttgccg gaggttctgc tcgtcgtgcg cggcaatttc agcgcagacg
      901 gcctgctctc cgccgcgcgc atggcagggc agggcatgta cgtgcaggag acttacggcg
      961 gaaagacctt aaacgtcttt acgctcaaca agcctgctac gacggctgac ggcagcggcg
     1021 gcgggggcgg cgaacagtcc gggagaaagt ttcccgtcga tcagatcgcg gcggtggctc
     1081 tcgatgccaa cacgctcgcc atcggcatcc ccgcttatct gcgcgatgcg attgacgcgg
     1141 gcgcgggcgg cggcacgacg gcgcggctca agcccgagtt ggttgatctc acgctgcgcg
     1201 atggcaacac gctcgtcagt ctcgtgaccg acgtgccgcc cgcgatgtcg cagcatttgc
     1261 gcctgctcgg aatgccgccg aacgccgaga cggacaggct aattgacgcc ttgcgtcagg
     1321 tgcagttgtc cgtgaacatg cagccgggaa ctttcggcgt gcaatcgatt ctgcggatgg
     1381 acagttccga atcggcttcg gcgctgaacg gtctggtcgg catgggcgca actctggcga
     1441 aggcgcagat cgcgaaagag gtaggaaaac agaccagcag cgggaaggat gacatggccg
     1501 ccctcctgcg tgtgcttgag acgttgacca acacggcgcg cgataacgaa gtcgtgctcg
     1561 gaatcacttt tcagcaagcc tctctcgcag aagtgatcaa gcgacagatg atgccgaaga
     1621 agcaggagac ggccattact cccaggccgg atggaaacaa gggcgcggcg gtgaaacgcc
     1681 gtaggccgcg cacgcgcaga aggtaaccgg gtgaattaga tgaacaaggg gcgatacacg
     1741 gaagcagagc acgacagttt cgcagaagcc gtattacgcg acggcttctg cctgctgccg
     1801 gggcatttct cgaaagagac tttggaagcg tggcgcgcgg ccttcgcgct gttgctcgaa
     1861 gagcatatcg aacgcgaagg ccacctgcgc aaccggggcg cggcgcgcta ctacgtgacg
     1921 ctgcctttca tcgccccgtt cgccgatcct gcgatctacg aagatgcgga cgtgctggcc
     1981 gtcatc
//
LOCUS       DASWNS010000056         5087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_210124, whole genome shotgun sequence.
ACCESSION   DASWNS010000056 DASWNS010000000
VERSION     DASWNS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210124"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1325)
                     /locus_tag="VGB76_02025"
     CDS             complement(261..1325)
                     /locus_tag="VGB76_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEY0097705.1"
                     /translation="MRVVKILFLFASICASVALPLQAQQPQQPQSTTAQPEIVAARRD
                     TSNSTMTVRREYLLGPNDVVELRVFGEPQFDGAFTVDGDGKLAVPFVEEPLAVQCRSV
                     KEIRKDVVTALSKFLKNPQVYMSVKDEKSRTPATVYGAVRSPAQYEMRRPARLLELLS
                     KSGGVTEQQSGTIQIFHTQPLLCPGPEEMNIAAAQVEGGEDTLGVPFTLYKVDELKMG
                     KNEANPYIHPGDVIYVAEAAPIYITGSVAQPANLYLRDKMTLQRALAIVGGPKNAKES
                     QVRIYRQKPGVLKPEILTVDYKAIKQGKKEDIALQPYDIIEVPENGVWSAKGVAKLLT
                     GLATNGAASMVTQGGARIIY"
     gene            complement(1653..2696)
                     /locus_tag="VGB76_02030"
     CDS             complement(1653..2696)
                     /locus_tag="VGB76_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097706.1"
                     /translation="MPIESANYTDSNRNARAAVAHASSGQRKRPPARPRSPSSFSFML
                     TAALIALFIFFALWWLLLTSGDETPWLPAALAASVVMLVAVSAREVVMRRAWARYTRE
                     LEMRMGSESAQVPSAALHPRPSRKATVHATANSLRALQQRLAKAEEAGAQQPEAHLEA
                     YRLCEQYLATTEDAMHKPGMGTDVRAALRAGQERVRGLQKHHLLSWVRGEAQRLTHEA
                     QRRVSVSDKIETAMRAIDVINEALQIYPAEPELRDSSLAVRNFIASVKVAHWVELAER
                     AAFRGRYTRAVARYRDALFYLSRAEMGEDAREEAATRINREIEMLRARLAMGDLEEEP
                     PLQRRPRPARKKP"
     gene            3099..3473
                     /locus_tag="VGB76_02035"
     CDS             3099..3473
                     /locus_tag="VGB76_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0097707.1"
                     /translation="MVNCPKCGVGVREEKAFCHNCGAPMNDAMRQREEPQPDFAATVL
                     EPPRRAAQPFVPPSITEVQPTAGETATVGARQPAAPAALPLPPGAQPNGRRKSSRKIG
                     FGFVILLLFLMFLVFVLAVVLD"
     gene            3520..4674
                     /locus_tag="VGB76_02040"
     CDS             3520..4674
                     /locus_tag="VGB76_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEY0097708.1"
                     /translation="MNEFAGIPHRPHAALAAGRPRRSLLRGAAIVFASLVALLLALLL
                     LLLTGLQTGPVPFLIGFVLATLPAPLYVALVLWIDRYESEPVWTLVTAFAWGAIVAVF
                     IAAFFNTLSSLIVASLVNDEAGKLFGAVVSAPIVEESAKAAVLFALYFWKHDEFDGVI
                     DGIVYASMVGLGFAMTENIQYYGNAALEGGIEEGLALFILRGMLAPFSHPLFTSMTGI
                     ALGLATQTKNQLAKFFYPALGLLLAMFLHSLWNLSASINAALYFIVYFLIMVPVFVAV
                     LISIYFALRREGRIVREHLRHDLEHGRLTQFELEILASVRGRMGASFRALTSGGYRSW
                     RTRREFNRVASELAFHRNRVAQGRAVSDGAAVEELYVRQLTELRGQFNNS"
     gene            4710..>5087
                     /locus_tag="VGB76_02045"
     CDS             4710..>5087
                     /locus_tag="VGB76_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097709.1"
                     /translation="MKIQLLPSTFGQDGHASQEQRLTCFLIDDRVAVDAGSIALGLSD
                     AQHETVRDVIVTHPHMDHMATLPIFIDDHFALLTEPVRVHATEEVITLLERDIFNWTV
                     YPRFSQLKNEHGAVMEYVPFPVRE"
BASE COUNT          900 a         1582 c         1531 g         1074 t
ORIGIN      
        1 gatcaagccg acgataatca caaccgctac ggcgatccat atgagcgtca tccggcgacc
       61 gcctttgttg cgcacgttcc ccgttgttgt cccagccatc ttagttctcc cgactaaatg
      121 aaaatgaaaa ttggaaaggc gttcttaact tgaacgcagc gcgcaggcag acacttcacg
      181 ctcgcgcctc gttcactcac gcgccgcaga gagtttgacg aactctttcc gtcaaactct
      241 cgcggcagcg attttcgtgt ctaataaatg atgcgcgccc cgccctgcgt gaccatagaa
      301 gcggcaccgt tggtagccag gccggtgagc aacttcgcga cgccctttgc cgaccagacg
      361 ccgttctccg gcacttcgat gatgtcgtaa ggttgaagcg cgatgtcctc tttcttgcct
      421 tgcttgatgg ccttgtagtc aaccgtcagt atctccggct tcaacacgcc gggcttctgc
      481 ctgtaaattc gcacctgact ctctttggca ttcttcgggc cgccgacgat agccaacgcg
      541 cgctggagcg tcatcttatc ccgcaggtaa agattggcag gctgcgccac actgcccgtg
      601 atatagatcg gcgcggcttc ggccacatag atcacgtcgc cgggatgaat gtaggggttg
      661 gcctcgttct tgcccatttt aagttcatca actttgtaaa gcgtgaaagg cacgccgagc
      721 gtgtcctcgc cgccttcgac ttgcgcggcg gcgatgttca tctcttcggg gccgggacag
      781 aggagcggct gcgtgtggaa aatctggatc gtgccgctct gctgttcggt gacgccgccg
      841 ctcttcgaga gcagttccaa gagacgcgcg ggacggcgca tctcgtactg ggcgggcgag
      901 cgcaccgcgc catagacggt cgcgggcgtc cggctcttct cgtccttgac agacatgtag
      961 acctgcggat ttttgagaaa cttcgagagc gcggtcacga catctttgcg aatttccttg
     1021 acgctgcggc actgcaccgc gagcggctct tccacgaacg gcacggcgag tttgccgtcg
     1081 ccgtcaacgg tgaacgcgcc gtcgaactgc ggttcgccga aaactctcaa ttcgaccacg
     1141 tcgttcggtc cgagcaggta ttcgcgccgc acggtcatcg tgctgttgga cgtgtcccgc
     1201 cgggcggcga cgatttccgg ttgcgccgtc gtgctctgtg gttgctgcgg ttgctgcgct
     1261 tgcagcggca gggcgaccga agcacagatg gaggcgaaca gaaataaaat tttaacaact
     1321 ctcatgtttg tatgtcctgt cagtcgaatg tggcgcaagt gtcgaatgtc acggtttacg
     1381 gagcgtgcgc gcggaagact tatagatgta cgaaagaagt taaagggctg ccttgaggcc
     1441 aacttttaat ttgccgggga ggaattaaag cgcgccgcat gcccttcgcc aactatcgtt
     1501 taaatcgttc tgcgcacgtc gattgtcgca cagcaagcgg ccaaatgcca attcacggtc
     1561 ggggatggtt tcgacccgtc gcgtcgtttg cgccacgact cgcgcggcgg acgacgcgga
     1621 tttgcgtcgt gtagagtttt gccgttcgcg cgttaaggct tttttcgcgc gggacgcggg
     1681 cggcgttgta gcgggggttc ctcttccaga tcgcccatcg ccagacgcgc acgcagcatc
     1741 tctatctcgc ggttgatgcg tgtggcggct tcctcgcggg cgtcttcgcc catctcggcg
     1801 cgggagagat aaaagagggc gtcgcggtag cgtgcgacgg cgcgggtgta gcgtccgcga
     1861 aaagcggcgc gctcggcgag ttccacccaa tgcgcgacct tgacggaagc gatgaagttg
     1921 cgcacggcga gcgaagagtc gcgcagttcc ggttccgccg ggtagatttg caacgcttcg
     1981 ttgatgacat ctatggcgcg catggccgtc tcgatcttgt cggagacgct cacgcgccgc
     2041 tgggcttcgt gcgtgagcct ttgcgcctcg ccgcgcaccc atgagagcag gtgatgcttt
     2101 tgcagcccgc gcacacgctc ctgtccggcg cgcagcgcgg cgcgcacgtc cgttcccatg
     2161 cccggcttat gcatcgcatc ttccgtcgtc gccaaatact gttcgcagag gcgataggct
     2221 tcgaggtggg cttcgggttg ctgtgcgccc gcctcctccg ccttcgcgag cctctgctgc
     2281 aaggcgcgca gggagttggc cgtcgcgtgg acggtcgctt tgcgtgaggg gcgcgggtgg
     2341 agtgcagccg atgggacttg cgcgctctcg ctgcccatgc gcatttcgag ttcgcgcgtg
     2401 taacgcgccc aggcgcggcg cattacaacc tcgcgcgccg acaccgccac gagcatcacg
     2461 acgctcgcgg ccagagcggc gggcagccag ggcgtctcgt cgccgctcgt caggagcagc
     2521 caccataaag cgaaaaagat gaagagggcg atgagagccg ccgtaagcat gaagctaaag
     2581 gacgaggggc ttcggggacg ggcgggcgga cgcttgcgct ggccggagga agcgtgagcg
     2641 acagcggcgc gcgcgttgcg atttgaatct gtgtagttag ccgattcgat gggcaagcct
     2701 atctctccgg agacgagggg aactggcgac tcgcgtggcc gcgctctcta tggctcgcgc
     2761 cggcgcgtga aactggcgat tctcttaggt tgcgggcgcg aaacgcctat cagtgtgaaa
     2821 cggaaatcat agtcaccttt tcgtccctgt ctgtcaacag tcgagatgac ggtcgggctg
     2881 ttaccagagc cgccgcgccg ggttgaaatt atactggaag tggggggcaa atctgtttat
     2941 actggcgcgc gttcgtgaac ttcgcatgcg ccgcccggtt gaatggccgg ggttcaatcg
     3001 tccccgaatt cttaaacacc gcgcaattat agaatttccc gccgggttgc aggcgcgggc
     3061 aagtgcgttt cgagttgcac ggtggatgca tcaagctcat ggtcaattgt ccaaaatgcg
     3121 gcgtgggcgt gcgtgaggag aaggcttttt gccataactg cggcgcgccg atgaatgacg
     3181 cgatgcggca gcgcgaagag ccacagcctg acttcgccgc gaccgtgctc gaaccgccgc
     3241 gacgcgccgc gcagcccttc gtgcccccgt cgatcaccga agttcaaccg accgccggag
     3301 agacggcgac cgtcggcgcg cgccaacccg ctgctcccgc cgccttaccc ttgccccccg
     3361 gcgcgcagcc aaacggcagg cgaaagtcct cccgaaaaat cggcttcggc ttcgtcatac
     3421 ttttgctctt cctgatgttc ctggtgtttg tgctcgctgt cgtgctcgac taatctcttc
     3481 gcgcaagtta accctcgaag gcgacccgca aaacttccga tgaacgaatt tgcaggcatc
     3541 ccgcatagac cgcacgccgc tctcgcagcg gggcgacccc gccgcagcct cttgcgcggc
     3601 gcggcgattg tgttcgcctc gctcgtcgcg ctcctgctcg cgctgctcct gctgctgctc
     3661 acgggactcc agacaggccc cgttcccttc ctcatcggat tcgtgctggc gacgctgccc
     3721 gcgcctctct acgtcgcgct cgtcctctgg atcgaccgct acgaatccga acccgtctgg
     3781 acgctcgtaa ctgcttttgc atggggcgcg attgtggccg tcttcatcgc cgccttcttc
     3841 aacacgctca gcagcctcat cgtcgcctcg ctcgtcaacg atgaagcggg caaactcttc
     3901 ggcgcagtcg tctccgcccc catcgtcgag gagagcgcga aggccgccgt gctcttcgca
     3961 ctctacttct ggaagcacga cgagtttgac ggcgtgatcg acggcatcgt ctacgcttcg
     4021 atggtcggcc tcggtttcgc catgaccgag aacatacagt attacggcaa cgcggctttg
     4081 gaggggggca ttgaagaggg actcgcgctc tttatcctgc gcggcatgct cgcgcccttt
     4141 tctcatccgc tcttcacgag catgacgggc atcgctctgg gcttggcgac gcagacgaaa
     4201 aaccaactcg ccaaattctt ctatcccgcg ctcggcctcc tgctcgcgat gtttctccac
     4261 agcctgtgga acctttcggc ttcaatcaac gccgcgctct acttcatcgt ctattttctg
     4321 atcatggttc cggtcttcgt cgcggtctta atctcgatct acttcgcgct ccggcgtgag
     4381 ggtcgcatcg tgcgcgagca tctgcgacac gatcttgagc acgggcgtct gacacagttt
     4441 gaactggaga tactggcgag cgtcagagga cgcatgggcg cgtctttcag agcgttgacg
     4501 agcggcggct atcgctcctg gcgcacgcgc cgggagttca accgggtggc gagcgagttg
     4561 gcttttcatc gcaaccgcgt ggcgcaaggg cgtgcggtca gtgacggtgc cgccgtcgaa
     4621 gaactatatg tccggcaact cacggaactg cgcggtcagt tcaacaactc ttgaagacgg
     4681 agcgcgacac ttaagacgga acttccctcg tgaaaatcca acttcttccc agcaccttcg
     4741 gccaggacgg ccacgccagc caggaacagc ggctcacctg ttttctcatt gacgaccgcg
     4801 tcgccgtgga tgccggaagc atcgccctcg gcctctccga cgcgcagcat gaaaccgtcc
     4861 gcgatgtcat cgtcacgcac ccgcacatgg atcacatggc gacgctgccc atcttcatcg
     4921 acgatcattt cgcgctcctg accgaacccg tgcgcgtgca cgccaccgaa gaggtgatca
     4981 cgctgctcga acgcgacatc tttaactgga ctgtctaccc gcgcttctcc caactcaaga
     5041 acgaacatgg tgcggtgatg gaatacgtcc cgttccccgt gcgcgag
//
LOCUS       DASWNS010000057         9858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_53932, whole genome shotgun sequence.
ACCESSION   DASWNS010000057 DASWNS010000000
VERSION     DASWNS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53932"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VGB76_02050"
     CDS             <1..407
                     /locus_tag="VGB76_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097710.1"
                     /translation="EKRIKTFVLMAGSLSDADDLRSDHPEMVKLRESVGREKIEQIIR
                     ETNWQNPANFIGHAAPASVLLQYAYEDGFLDEARARRYFALVSEPKELKFYTAGHALN
                     AEARRDRYEWLRLKINLRKLKRAVLDQVKEVK"
     gene            435..1859
                     /locus_tag="VGB76_02055"
     CDS             435..1859
                     /locus_tag="VGB76_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097711.1"
                     /translation="MILQTKIRSRPALTHGLALAAFSAAIFLPLIGRGFVHDDFMWLA
                     SVAHETRWHGLTHPTPTFYTPLTWLSFKLDWTLWGLRPFPYGLENLLLHILNTLLLYR
                     LALRLWQRHTAAWWAAFGFALLYMANSWAVMWISARTHLLATLFCLAAMLATVRYVQS
                     ERRKVTAVLTVVVCCALSMLAKEIGVASIGACFIVLWYAGGFRRLRSSWAELALLSGA
                     LLAVLCAFLMLRAWAGALSVSSNQGWYQYAFDLKVLFLNLREYLSRTYLVAALIAGAI
                     ALSQAIRGSKPTLNAVTRRELFLSVMLFATTIAPVILIRGRSGLYTYLPGTGAALLLG
                     AAACSLYPAGDNTRRLTWVNLLPVILLSALLSLATVGQSWKWRTMAKTNSSILRQLAE
                     QDPQPARDTHIVLSYAQPDRRHRFPDGFGTWGFPFAVRLLYNEPNLRGDIVSEANQSE
                     PVAGSTIVNYLYVAGEDSPKVVKQ"
     gene            complement(1906..3822)
                     /locus_tag="VGB76_02060"
     CDS             complement(1906..3822)
                     /locus_tag="VGB76_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY0097712.1"
                     /translation="MHILSIESLSKNYGLKPLFENVTLGFDSQDRIGVVGANGSGKTT
                     LLRLIAGAEQPDSGRVVFAEGVSVGYLPQNPPYEAEQTVLDAVFAASDAKMKLLLDYE
                     RACQLLAASGGGADEPRLLNRVSELAHKLEATGAWELETNARMVLSRLGIQDTAARMG
                     ALSGGQRKRVALAHALIANPQLLILDEPTNHLDAETITWLEMYLARYRGALLLVTHDR
                     YFLDRVTGRILEIDRNIVQAFDGNYAYYLEKKEEQELARAAEGQKREMLIRRELAWLR
                     RGAKARTRKSKARIDRAESLMAQPREATRAELDISVTTSRIGKKILELRDIRKAYDGR
                     ILIDNFSYTVKPGDRIGIIGANGAGKTTLLEIITGRIKPDSGSLETGQTVRIGYYDQE
                     NRALNDEQRVIDYIREVAERIETADGAFITAGQMLEKFLFPGALAYAPIAKLSGGERR
                     RLYLLRVLMNAPNVLILDEPTNDLDIQTMVTLEDYLDSFGGCLIVVSHDRYFLDRTVE
                     HIFRFEAGGKVREYPGNYAAFLEARQKEERAVATPTDGNETAMRAVKKERTAQAAAPP
                     AKRKLSFKEQRELEELEARIQTAEQRQAEIETELAANSSDAHLVNKLFQERETLALEL
                     TRALDRWAELAELA"
     gene            3942..4481
                     /gene="msrB"
                     /locus_tag="VGB76_02065"
     CDS             3942..4481
                     /gene="msrB"
                     /locus_tag="VGB76_02065"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0097713.1"
                     /translation="MNRRYFIKSGATLGAAALLCKLPFVSASQQKGSTGEGVASASIK
                     KIVKSDEEWRRLLTPEQYRVTRQKGTETPYSSPLNKVSARGVFECVCCALPLFSSQAK
                     YDSHTGWPSFWKPISKQNVREQTETAEGDTRTEVVCNRCDAHLGHVFDDGPDPTGLRY
                     CMNGVALKFVKSAKSVKRA"
     gene            4542..4940
                     /gene="msrB"
                     /locus_tag="VGB76_02070"
     CDS             4542..4940
                     /gene="msrB"
                     /locus_tag="VGB76_02070"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0097714.1"
                     /translation="MTKKVIKTEEEWRASLTPEQYRVARQKGTERPFTGEYYDAKDEG
                     IYKCAACGNELFSSEAKFDSGCGWPSFTEPKAAENVRTEMDYSHGMKRTEVLCAACDS
                     HLGHVFDDGPRPTNLRYCINSVVLDLDKKE"
     gene            complement(5037..5921)
                     /locus_tag="VGB76_02075"
     CDS             complement(5037..5921)
                     /locus_tag="VGB76_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097715.1"
                     /translation="MAQNGKLELELVDVHGQFLDERVDVMLRHRVLSHFVKKTQKASA
                     KFTIPNLHAEPQGLYRIESVPLSYMPVDDNIKIKGSGTTPYRAVFPVDPKKVIKVNFP
                     RYDKLPPDALTLLSRSDKVLAFEGKTGDTLYTALEKENLKLAGMLNIIAKTNLARLSN
                     GRTVLSYLQELRELRGDRFFVVASKELREETKHSVAEGLFAKKSGKLHHLPAQFIGYE
                     PAGSFKTPDRYGNLQLTFFMKGDDCVADIDIDDASGLQHIFQVLNNMLPGNDTHPYTI
                     HEILIASQKLDPGYTFVL"
     gene            complement(6014..7792)
                     /locus_tag="VGB76_02080"
     CDS             complement(6014..7792)
                     /locus_tag="VGB76_02080"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY0097716.1"
                     /translation="MPMNFDAIVIGTGFGGTIAATRLAAKGKKVLLLERGTWWVTPAK
                     LGKPPASTRPSIPAWAKEQKPPQPVQYWTRPDHKDGMLDFFAAIRSPFNKDGLYQYSI
                     FKQADILTASGVGGGSLIYSNVTVRPQAEVLQGIGLNLGDADYQLAYKWMEDFRGRLN
                     HIVTKIPLPGRDVTNLTTDDYLYLDRARVLRDAAKLSEQKLGIKLPWKPLDLSLIEYD
                     PHRADGGDAAKVGTFCQRQGRCILGCLPSARHTFSKTLYTKFLSDPSKGVTLSPLAEV
                     RDIKQIAGGYEVNYRDHRDDEKKTVSAPLVFLAGGTLGTTEILLRSRERGGLKLSDTL
                     GSHFSTNGDFGAFVVGTTKPVHSTVGPINTCQVSTKLDGLHIKVEDSAIPEMFAALAS
                     TTLGVLDNFAQREMLKGRLKFAFINKALPDLRDFFPHLPNTYDPESNQTEAEMVSNIF
                     FFNVMGQDDASGRFTLKNDGLDLDWGKKIGETPIFQKIEVLLKSLAESMGGRYVPFPL
                     WKGLGNKKLVVTHPLGGCPIAPTSADGVVDELGRVFDGSKPKGAKDILPGLFVVDGSA
                     IPGAVATNPTLTIAAQALKSINAALP"
     gene            8279..9034
                     /locus_tag="VGB76_02085"
     CDS             8279..9034
                     /locus_tag="VGB76_02085"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEY0097717.1"
                     /translation="MSKILNIGVLGGMGPEATNQLCALLTANTAVSRDQDHIPVITYN
                     NPRIPGRVAAVRGDDGALSPVPEMIRTAQVLERAGAGLLLLPCNLAHYFIADVQASIR
                     IPILNMIEETVNFIVEHYPQLRRAGILASTPTIECGLYDRAFRRHDKHLLAPEAVEQD
                     DKVMRAIYGSEGIKCGHKSAPRNLLKSAAENLSQRGAEVIIAGCTEVSLVLSRENSSF
                     NVIDPLEIIARVAIAHAGAGEVKLTRRDSATAN"
     gene            9077..>9858
                     /locus_tag="VGB76_02090"
     CDS             9077..>9858
                     /locus_tag="VGB76_02090"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097718.1"
                     /translation="MSNILLTLAEEREFTRDDFFAVKMKVDDGDCYDFSNELQSLGHE
                     VFFANWDDLDGREFTRMFHDNEKRFVSPLPLGDADLIFVYKMEGFYFDLPRFFQMVET
                     FAASARIVVNHPATIRHNIDKRYLLELERVGVPVPPTRLIDETVRERVSRGERLVLKP
                     VSGERGKDVMLADTLAALDSIKGREEEYLAQAYIPEVLAGERSLVFLGFEYQHAVLKR
                     PSPRNPQEFRCNESRGGTVTHYEPTRAELDFALRTLRAYESLG"
BASE COUNT         1970 a         2773 c         3001 g         2114 t
ORIGIN      
        1 tggagaagcg catcaagaca ttcgtgctca tggccgggag cttgtcggat gcggatgatc
       61 tgcgttcgga tcatcccgag atggtgaagc tgcgcgagtc tgtgggcagg gagaagatcg
      121 agcagatcat acgcgagacg aactggcaga acccggcaaa cttcatcggc cacgctgcgc
      181 ccgcgtcggt cttgctgcaa tatgcctacg aagacggctt tctggatgaa gcgcgcgccc
      241 gccgctattt cgcgctcgtg agcgaaccga aggagttgaa gttttacaca gcggggcacg
      301 ccttgaacgc ggaggcgcga cgcgaccgtt acgaatggct gcgcctcaag atcaatttac
      361 gaaaactaaa acgcgccgtt ctggatcagg tcaaagaagt gaaataggaa tgcgatccgg
      421 cgtcgaatgc taacttgata cttcaaacga aaatcaggag tcgtcccgct ctcacgcatg
      481 gtctggcgtt agccgctttt agtgccgcga tatttctgcc gctcatcggg aggggcttcg
      541 tccacgacga tttcatgtgg ctcgcaagcg tcgcacacga gacgcgctgg catggactaa
      601 cgcacccgac gcccactttc tacacgcctc tcacgtggct ctccttcaaa ctcgactgga
      661 cgctctgggg gcttcgccct ttcccctacg ggctggagaa tctgctgctc cacatcctca
      721 acacgctctt gctctacagg ctcgcgctgc gcttgtggca gaggcacacg gcagcatggt
      781 gggcagcctt cggtttcgcg ctgctctaca tggcgaacag ttgggcggtg atgtggattt
      841 ccgcgcggac gcacttgctg gcaactctct tctgtctcgc ggcgatgctc gcaactgtcc
      901 gttacgtgca gtcggaacgg cgaaaagtaa cagccgtttt aacggtcgtc gtatgctgcg
      961 cgctctccat gctggcgaaa gagatcggcg tggcttccat cggcgcgtgc ttcatcgtgc
     1021 tctggtacgc gggaggtttc cggcggctga gaagttcatg ggcggagttg gcgttgctct
     1081 ctggcgcgct gctcgctgtg ctctgcgcct ttctcatgtt gcgcgcgtgg gcgggcgcac
     1141 tctccgtctc gtcgaaccaa gggtggtatc aatacgcgtt cgacctgaaa gtgctatttt
     1201 tgaatttgcg tgagtaccta tcgagaacct acctcgtcgc ggcgttaatc gccggagcca
     1261 tcgcgctctc gcaggcaatt cgcggcagca agcctacttt aaacgcggtc acacggcgcg
     1321 aacttttttt gagcgtgatg cttttcgcca cgacgattgc gcctgtgatt ctgattcgcg
     1381 gaaggtctgg actctacaca tatctgcccg gaaccggcgc ggctctcctg ctcggcgcag
     1441 ccgcatgctc gctctacccg gcgggtgata acacacgtcg tctgacgtgg gtaaacctgc
     1501 ttcccgttat cctgctgtcc gcactgctga gtctggcaac cgtggggcag agttggaaat
     1561 ggcggacgat ggcgaagacc aactcgtcca tcctgcgaca gcttgcggag caagacccgc
     1621 agcccgcacg cgacacgcat atcgttttaa gttacgcgca gccggaccgg aggcatcgct
     1681 tcccggacgg tttcggcacg tggggcttcc ccttcgccgt gagacttctg tacaacgagc
     1741 cgaacctgcg cggcgacatc gtgagcgaag cgaaccagtc tgagccggtc gcgggcagca
     1801 cgatagtcaa ttacttgtat gtcgctggcg aggacagccc gaaagtcgtt aagcaatgat
     1861 gaggacaaga gagtaaggat gaacagttgg aagagcgttg atctctcagg cgagttcggc
     1921 gagttctgcc caacggtcga gggcgcgtgt gagttcgagt gcgagcgtct cgcgctcctg
     1981 aaagagtttg ttgacgagat gcgcgtcgct cgaattggcc gccaactcag tctcaatctc
     2041 agcctgacgc tgttcagccg tttgaatcct cgcctccaat tcctccaact cgcgttgctc
     2101 tttgaatgat aacttacgct tggcaggcgg cgcggcggcc tgtgccgtcc tctccttttt
     2161 gacggcgcgc atcgcggttt cgttcccgtc cgtcggtgtg gcgactgcac gctcctcctt
     2221 ctgccgcgct tccagaaagg cggcgtagtt gcccggatac tcgcgcacct tgccgcccgc
     2281 ctcgaagcgg aagatatgct cgacggtgcg gtcgagaaaa tagcggtcgt ggctgacgac
     2341 gatcaggcag ccgccgaagc tgtcgagata atcttcgagc gtgaccatcg tttgaatgtc
     2401 gagatcgttt gtcggttcat ccagaatgag gacgttcggc gcgttcatca gcacgcgcag
     2461 gagatagaga cgcctgcgtt cgccgcccga taatttcgca atcggcgcgt aagcgagcgc
     2521 gccggggaag agaaactttt ccagcatttg cccggcggtg ataaacgcgc cgtcggcggt
     2581 ttcgattcgt tcggccactt cgcgtatgta atcgatcacg cgctgctcgt cgttgagcgc
     2641 gcggttttcc tgatcgtaat agccgatgcg gactgtctgc cccgtttcga gcgagccgct
     2701 atcgggcttg atgcgcccgg tgatgatctc aagcaaggtg gtcttgcctg cgccgtttgc
     2761 accgatgatg ccgatgcggt cgccgggttt gacggtgtag ctgaaattgt cgatcaggat
     2821 gcgcccatcg taagccttcc tgatgtcgcg cagttctaaa atcttcttgc cgatgcggct
     2881 ggtcgtgacg gagatgtcga gttcggcgcg cgtagcctct ctcggctgcg ccatcaaact
     2941 ctcggcgcga tcaatacgcg ctttagactt acgcgtgcgc gcctttgcgc cgcgacgcag
     3001 ccatgccagt tcgcgccgga tgagcatctc gcgcttctgt ccttcggcgg cgcgtgcgag
     3061 ttcctgctcc tctttcttct ccagatagta agcgtagttt ccgtcgaacg cctgcacgat
     3121 gttccggtcg atctcaagga tgcgcccggt tacgcggtcg aggaagtagc ggtcgtgcgt
     3181 gacgagcagg agcgcgcccc tgtagcgtgc gagatacatt tcgagccacg tgatggtttc
     3241 ggcatcgaga tggttggtcg gttcgtcgag gatgagaagc tgcggattgg cgatgagggc
     3301 gtgagcgagc gcgacgcgtt tgcgctgccc gccggagagc gcgcccatgc gtgcggcggt
     3361 gtcctgaatg ccgagacggc tgagcaccat gcgcgcgttg gtttccagtt cccatgcgcc
     3421 cgtggcctcc agtttgtgcg ccaactccga gacgcgattc aacaggcgcg gctcgtccgc
     3481 accgccgccg gacgcggcca acaactggca cgcgcgctcg taatcgagaa gcaacttcat
     3541 cttcgcatcg ctcgccgcaa agacggcatc gagcacggtc tgttccgctt cgtagggcgg
     3601 gttctgcggc aggtaaccga ccgacacgcc ctctgcgaaa actacgcgac cggaatcggg
     3661 ctgctccgcg cccgcgatga gacgcagcag agtggtcttg cccgaaccgt ttgcgccgac
     3721 tacgccgatg cggtcttgcg aatcgaatcc gagcgtgacg ttttcgaaga gcggctttag
     3781 tccgtagttc ttgctgagcg attcgatgga tagaatatgc atgaagggtt tgttaaaata
     3841 accgacatta gctctgaaaa gcgttcgcgt tacattcccg gtcaatccaa gtcttcattc
     3901 aatacgtaaa tagaatgaaa tccacgtaga gaggttggga tatgaataga cgttatttta
     3961 tcaaatccgg cgcgactctc ggagcagccg cgctgttgtg caaactgccg ttcgtttcgg
     4021 catcgcagca aaaaggctcg acgggtgaag gcgtcgcttc cgcgtccatt aaaaagatcg
     4081 tgaagagcga tgaagaatgg agacgcctgt tgacgccgga gcaataccgc gtcacgcgac
     4141 aaaaggggac ggaaactcct tattccagcc cgctcaataa agtctctgcg cggggcgtgt
     4201 tcgagtgcgt ctgctgcgcg ctgcccttgt tcagttcgca ggcgaaatac gattctcaca
     4261 cgggctggcc gagtttctgg aagcccatct ccaaacagaa cgtgcgcgag caaacggaga
     4321 cagccgaggg cgacacgcgc acggaagtcg tctgcaatcg ttgcgacgcg catctcggtc
     4381 acgtcttcga cgacgggcct gacccgaccg ggctgcgcta ttgtatgaac ggcgtggcct
     4441 tgaagtttgt gaaatcagcg aaaagcgtaa aacgcgcctg agacttcctg cgcgggcgga
     4501 agttaatttg gcgatcattt aagaaccgga aggattgatt cgtgaccaag aaagttatca
     4561 agacggaaga ggaatggcgc gcgagcctga cgcccgaaca atatcgcgtc gcgcggcaga
     4621 agggaacgga gcgaccgttc acaggcgaat actacgatgc gaaggacgaa ggcatctaca
     4681 agtgtgcggc ttgcggcaac gaactgttta gctccgaagc caaatttgat tcgggctgcg
     4741 ggtggccgag tttcaccgag ccgaaggcgg cggaaaacgt ccgcaccgag atggattaca
     4801 gccacggcat gaaacgcacg gaagttttgt gcgccgcttg cgattcgcat ctcggccacg
     4861 tcttcgacga cgggccgcgc ccgaccaacc tgcgttattg catcaattcg gtcgtcctcg
     4921 atctagacaa aaaagagtaa cgcgccgttc gcgcaagtct cgacaggctt gagcctgaga
     4981 aacgtgcgcg agctttggcg cgcgacacag gcagccggct acgaaccgaa attctcctag
     5041 aggacgaagg tgtagccggg gtctaacttc tggctggcga taaggatttc gtgaatggtg
     5101 taagggtgcg tgtcgttgcc gggcagcatg ttgttgagca cctgaaagat atgctgcaaa
     5161 ccggaagcgt cgtcgatgtc gatatcggcc acgcagtcgt cgcccttcat aaaaaaagtg
     5221 agttgcagat ttccgtagcg gtcgggcgtc ttgaaactcc cggcgggttc gtagccgata
     5281 aactgcgcgg gcagatggtg cagcttgccg ctcttctttg cgaacagacc ttcggccacg
     5341 ctatgtttag tctcctcgcg caactccttc gaggccacca cgaaaaaacg atccccgcgc
     5401 aactctcgca actcttgcag gtaagacaag accgttctgc cgttgcttaa tcgagccaga
     5461 ttcgtcttgg cgatgatgtt gagcattccc gccagcttga gattttcctt ctccagagca
     5521 gtgtagagcg tgtctcccgt cttgccttcg aacgcgagaa ctttgtcgct ccggctgagt
     5581 agagtcagcg catcgggcgg gagtttatcg tacctgggaa agttcacctt gatgaccttc
     5641 ttcggatcga cgggaaatac cgctcggtaa ggcgtcgtgc cgctgccctt gatcttgata
     5701 ttgtcgtcca caggcatgta agacaacgga acgctctcga tgcgataaag cccctgcggc
     5761 tcggcgtgca aattcgggat agtaaatttc gccgacgcct tctgggtctt cttaacaaag
     5821 tgactcaaga cacggtggcg cagcatgaca tcaactctct cgtcgaggaa ttgaccgtgc
     5881 acgtccacca actccaattc cagtttaccg ttctgtgcca taacctgatt tctctcctct
     5941 ccaattttgc gcccggcagg cttaaccgaa accaacgctc cagcctgtct tcaaactgct
     6001 cgcgccggag tgctcacggt aatgccgcgt tgatggattt caatgcctgc gcggcaatcg
     6061 tcagggtcgg gttggtggcg accgcgccgg ggatggcgga accgtcaacg acgaacaagc
     6121 ccggcaggat atccttcgcg cctttgggtt tgcttccatc gaacacgcga ccgagttcgt
     6181 caactactcc gtccgcgctc gtcggggcga tggggcaacc gcccagagga tgcgtcacga
     6241 cgagtttctt gttgcccaga cccttccaga gcgggaacgg cacgtaacgc ccgcccatcg
     6301 actcggctaa ggatttcagc agcacttcaa tcttctggaa gatgggcgtc tcaccgatct
     6361 tcttgcccca atcgagatcg agtccgtcgt tcttcaaagt gaacctgccg ctcgcgtcgt
     6421 cctgtcccat cacgttgaaa aagaagatgt tggaaaccat ctcggcttcg gtctggttgc
     6481 tttcggggtc gtaggtgttg ggcaggtgcg ggaagaaatc gcgcaggtcg ggcagagcct
     6541 tgttgatgaa agcgaacttg agccgcccct tgagcatctc gcgctgcgcg aagttgtcga
     6601 gcacgccgag cgtcgtgctg gcgagggcgg cgaacatttc ggggatggca gagtcttcca
     6661 ccttgatgtg cagcccgtcg agtttcgtgc tcacctgaca ggtgttgata ggaccaaccg
     6721 tgctgtggac gggcttcgtc gtgccgacga cgaacgcgcc gaagtcgccg ttggtggaga
     6781 agtgcgagcc gagcgtgtcg ctgagtttga ggccgccgcg ctcacgcgaa cgcaaaagta
     6841 tctcggtcgt gccgagcgtg ccgcctgcga gaaagacgag cggggcagag acggtcttct
     6901 tctcgtcgtc gcggtgatcg cggtagttca cttcgtagcc cccggcgatc tgcttgatgt
     6961 ccctgacctc ggccagcggc gagagcgtga cgcctttaga ggggtcggag agaaacttcg
     7021 tgtagagcgt tttgctgaaa gtgtggcgcg cggagggcag gcagcccaga atgcagcgac
     7081 cctgacgctg gcagaacgtc ccgactttcg ccgcgtcgcc gccgtctgcg cggtgcggat
     7141 cgtattcgat gagcgagagg tcgagcggct tccaggggag cttgatgccg agcttctgct
     7201 cggacagctt ggcggcgtcg cgcagcacgc gtgcgcggtc gaggtagaga tagtcgtccg
     7261 tcgtcaaatt cgtcacgtcc cgaccgggca ggggaatttt agtgacgata tggttaagcc
     7321 tgccgcgaaa gtcttccatc cacttgtagg cgagttgata atcggcatcg ccgaggttga
     7381 ggccgatgcc ctgcaagact tcggcctgcg ggcgcacggt gacgttcgag tagatgagcg
     7441 acccgccgcc cacgccgctc gccgtgagga tgtcggcctg cttgaagatc gaatactggt
     7501 agagtccgtc cttgttgaaa gggctgcgga tggcggcgaa gaaatcgagc atcccgtctt
     7561 tgtggtccgg gcgcgtccag tattggacgg gctgcggggg cttctgctct ttcgcccacg
     7621 cggggatgga cgggcgcgtg ctcgcgggcg gcttgccgag cttcgcgggc gtcacccacc
     7681 acgtcccgcg ctccaacagc aagaccttct ttcccttcgc ggccagacgc gtagcggcaa
     7741 tggttccccc gaaacccgtc ccgatcacaa ttgcatcgaa gttcattggc atggctatca
     7801 ctcctttatg gcaagcctct gcggcggtga ggacgactcg ctgaatgtga aaagcgaatc
     7861 gaacagattg gcgaaaagag ttgcgccacg ttaggcgttg aaggttgcct cgacgggcga
     7921 taggacgtag ggctttgatg gcgagggtcg gctggctatc aaagtcactt gcggaacgaa
     7981 ctcacagctt gcgcacttta ttcctgatga aaaaggctgt caagattttt ccacccgccc
     8041 gctggtttgt ctctcgacgg gcggcctctc gtttgcgcgc gtctggtggc ggcacggata
     8101 agtttttatg tttgacattc aattgtggcg agaatatctt ttcgcacgat ctaaccgttc
     8161 actataactg ccggagaaga ctcgctctta agttggcctc acccaaacac tcgacgcaag
     8221 cgaagcggcg aacaacgcga cacatacggc ggcgagagaa gcgtttgaag ttcttatcat
     8281 gtcgaaaatt ttaaacatcg gggtgctcgg cgggatgggg cctgaggcca cgaatcaact
     8341 gtgcgccttg ctcacggcca acaccgccgt cagccgcgat caggatcata tccccgtcat
     8401 cacatacaac aatccgcgca tccccggacg cgtggctgcc gtgcgcggcg acgacggggc
     8461 gttaagccct gtgccggaga tgatccgcac ggcgcaagtg ctggaacgag cgggcgcggg
     8521 attgcttttg ctgccctgca atctcgccca ctactttatc gcagacgtgc aggcgtctat
     8581 ccgcatcccc atcctgaata tgattgaaga gacggtgaac ttcatcgtcg agcattatcc
     8641 gcaacttcgc cgcgccggaa tactcgcttc gacgccgacc atcgagtgcg gcctctacga
     8701 cagggcgttt cgccgtcacg ataagcacct gctggcgcct gaagccgtcg agcaggacga
     8761 caaagtgatg agagcgatct acggcagcga aggcatcaag tgcgggcata agagcgcgcc
     8821 gcgcaattta ttgaagagcg cggcggagaa tctatcgcag cgaggcgcgg aagtcatcat
     8881 cgcgggctgt acggaagttt cgctcgtgct gtcgcgggag aattcgtctt tcaacgtcat
     8941 cgatcctctg gagattatcg cgcgtgtcgc catcgcgcac gccggtgcgg gcgaggttaa
     9001 gttaacgcga cgagatagtg cgacggcgaa ttgacaggcg taagcaaggc gaccctttca
     9061 tcggagaaga gagattgtga gcaacatcct gttgacgttg gccgaagaga gagaatttac
     9121 gagagacgac ttttttgcgg tgaagatgaa agttgacgac ggcgactgtt atgatttttc
     9181 aaacgaattg cagtcgctcg gacatgaagt tttcttcgcc aactgggacg acctcgacgg
     9241 gcgagagttt acgcgcatgt tccacgacaa cgagaagaga ttcgtctcgc ccctgccgct
     9301 cggcgatgcc gacctgatct tcgtctataa gatggaaggt ttctatttcg atcttcccag
     9361 attctttcag atggtggaga cgttcgccgc gagcgcgcga atcgtcgtca accatccggc
     9421 cacgatcagg cataacatag acaaaaggta tctgttggaa ttggagcgcg tgggggttcc
     9481 cgtgccgccg acgcgtctga tagacgaaac cgttcgagag cgtgtgtcgc gcggcgaaag
     9541 gctcgtgctc aaacccgtca gcggagagcg cggcaaggac gtgatgttgg cagacacgct
     9601 cgccgcgctc gattcgatca aggggcgcga ggaggaatat ctggcgcagg cttacatccc
     9661 ggaagttttg gcaggggagc ggagcctggt cttcctcgga tttgaatatc aacacgccgt
     9721 cttgaagcga ccttcgccgc gcaacccgca ggagtttcgt tgcaacgaat cgcggggcgg
     9781 aacggtgacg cactatgagc cgacgcgtgc ggagttggat ttcgcgctca ggacgctgcg
     9841 cgcttacgag tctctcgg
//
LOCUS       DASWNS010000058         8572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_27928, whole genome shotgun sequence.
ACCESSION   DASWNS010000058 DASWNS010000000
VERSION     DASWNS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27928"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VGB76_02095"
     CDS             complement(<1..372)
                     /locus_tag="VGB76_02095"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0097719.1"
                     /translation="MALLKIKDASGRQWQYPLSPQGICTIGRAIDNSVVLDDPRASRY
                     HAYIEFRDAAYHIIDGRKTGPLIQRSSNKVLIDGQPFQEHLLSNGAQIKIGASVLLFE
                     SSPVEAVMPRFSYDDRPLGHTQ"
     gene            complement(514..1914)
                     /locus_tag="VGB76_02100"
     CDS             complement(514..1914)
                     /locus_tag="VGB76_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097720.1"
                     /translation="MAEQSDDAQDAQTPQSPQETEEVTPQPPPPNRFWRALNPVISRV
                     PHTKAFHSRIAKGQESLREVRLEKYEQVTSVSPDVKSSLFVEFDRNTRQLSEVELWIE
                     MIQFYMGVLIGLIILSLFLVMPPLAAEFFPPFSDWNSHGLWNWSLEFLWSLLVAPLAF
                     AIIFVPFLVTTGAGAKVENFLNLDRLMALWASFLLLVASGYLYALYTGGMAAQPPLFR
                     QRPFFYFTLQTGFFLPTIFVLSVPGRLFFRSLESRKQAVQTRAFVIDELLNILHRHEE
                     PGYELIWSEFTHRKWLAERLEAIASCMENHFPRYFITGVPEMDRWATLTASEMANGVR
                     ATIKWVFAPTRNTREEFKARIVKYFIAALASEWGKFDRVTAEKLSRKEGLRARVVSVV
                     TALFTAAVPILLLLLVRRLQLVAEPLLTYLTVGSYIWAALSLLSHLDPHYNTKLAALK
                     DLTKTLPFGKRGGDDS"
     gene            complement(2180..3280)
                     /gene="ychF"
                     /locus_tag="VGB76_02105"
     CDS             complement(2180..3280)
                     /gene="ychF"
                     /locus_tag="VGB76_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY0097721.1"
                     /translation="MLRAGIVGLPNVGKSTLFNALTAQTSALAANYPFATIDPNIGVV
                     PVPDERLAPLARIVKTNTIVPATVEFVDIAGLVRGASKGEGLGNQFLANIRETDAVIQ
                     VVRCFEDDNIVHVEGSVNPERDIETIQIELALADLASVERRRERAQKNAKGGDKNALA
                     EIALIDKLLPVLSEGQPARLVPLTDDERATARTLFLLTMKPTIYAANVDEATLAAPDQ
                     NAHVKAVRALAGSEAAESVVICAQIEAELVELSPEDREGYLQSLGVEGSGVADLIKSA
                     YRLLGLMSFLTAGEKEVRAWTIPRGTRAPQAAGTIHSDIERGFIRAEIVSYDDLTRAG
                     SYAAAREQGLLRLEGKEYVMQEGDVVNFRFNV"
     gene            complement(3461..4033)
                     /locus_tag="VGB76_02110"
     CDS             complement(3461..4033)
                     /locus_tag="VGB76_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097722.1"
                     /translation="MKRLILIALLISAAAPVALPAQQPTAQQKEAARIATREQLRKLL
                     ETSGPKRGINIKFQQSEKQPFNFVGVLKDGLRNAESLEVVVGVTKDDTIGFRVYPHFK
                     GGYINADKAKNNVGLMRLLLNLSDRNFLFWGVDDSGDVFAGYTFTLESGFPEKSAEVV
                     LYSIPLLDKFVGEMRPFIDGSAAAGLALVR"
     gene            4288..6345
                     /locus_tag="VGB76_02115"
     CDS             4288..6345
                     /locus_tag="VGB76_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097723.1"
                     /translation="MASGALVTYLYDRDAHFFVRLAAGACTGFAALGLAGFVFASFLG
                     LTTASLILSAAVVAAPLTLLLKRDWRRRVRFDVGEAWRDVEAAIVRPEARTTGVLILF
                     FVSALIFWQVYERALFVRGGEILTGVDNNLGDLPFHLSIITGFVYGENFPPVHPEYAG
                     ARLTYPFVVDFVAAMFVRAGATLEGALFWENFVLALSLVGLLYRFALKLTRGDHLAAL
                     ITPALALLSGGFGWWMFVREWRGGTLGLFELLNRLPHDYTITSDGVFRWGNAITTLLV
                     PQRGLLLGLPLALIIITQWWLATEEEGDGGRGEGSKEEAGKEEAEPSIRRKGKGGKKE
                     KRSSPTATSEPPIPALNLFRLPFTLTPAVQRMIGAGVVAGLLPLVHAHSFVVLMLMGG
                     CLALLFPRWRLWFIFFALTLIIAAPQMWWATRQSAAQAGSFFGWQFGWDRGEQNALWF
                     WLNNTGLFIPLLIAAILWRGRAPVVSKRLLLFYLPFTLLFVISNAGKISPWVWDNIKV
                     LYYWYLASVPLVALLCARLWRLHAATRVAAIVLLLVLTLAGGLDVWRVVSEASEQREF
                     DRAGVAFAEIVKSQTAPRSLILHAPTYNHPVYLTGRRALMGYAGHLWSQGINYTEREA
                     ELRRMYAGAPDAESLMAKYGVEYAVVSPLERSGVAVNEQFFRRFTKVGETGGYSLYKL
                     TRR"
     gene            6311..8473
                     /locus_tag="VGB76_02120"
     CDS             6311..8473
                     /locus_tag="VGB76_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097724.1"
                     /translation="MATASTNSRAGKPKRRPTNVAQGNETGASSAAAPTTTTTGSHET
                     SGELSLRAWWIAYASVLILAALVRLYQLELRPMHHDEGVNGFFLNNLMRTGIFNYDPS
                     NYHGPTLYYFTLPLALLAQHFQMFDSWVVRLVPLIFGMATVWLALGLRRYIGAVGALA
                     AAALLALSPGVVFFSRYFIHEMMFVFFTLAVVVAALRLYETRGREEASPAKRWLELLI
                     LLAALPVVAQYATHIAQFALNLSGIFSGTANFAFNLPGYLVIFGLVALLVLVVLYERA
                     RAAYLILGAVAAALLFATKETAFISVGVLLIAWAMTWGYASIAGRQAWLGGGEPLSGR
                     ARRKQGLKGSATAPVSDSDSRGLVERFGGWRRLSLLSVAALGIFFSVNILFYSSFFSN
                     PKGVADSLKAFDIWTKTGKSEFHAKPFLTYVKWLLVEESPVLALSSMGALVALWRVRR
                     NRFALFAALWGFGLLAAYSLINYKTPWLALSMLVPLTLVGGYAINSIYRADEKPGLWR
                     VVALSLFCFALLTMFELFIALVQPKSLMPYLFINYKTLWPWVGMTLLFAGMGSYALNQ
                     SYKTGGTGRAGRVFALATLALALVVCAYQTYMINLVHYDNDAYIYVYAHTKRSYVELI
                     DEVERVAARAGTKFETPITVTAPEYWPMPWYLRDYKNVGYHGRLTPANNAAAIIASKA
                     QQSEVLAANGTRYLLVGDYEMRPGVMLTLYVRRDLAGR"
BASE COUNT         1532 a         2558 c         2690 g         1792 t
ORIGIN      
        1 ctgcgtgtgg ccgagtggcc ggtcgtcgta actgaagcgc ggcatcaccg cttccacggg
       61 cgacgactcg aacagcagga cgctcgcgcc aatcttgatc tgcgcgccgt tcgacagcag
      121 gtgctcctga aacggctggc cgtcaatcaa gaccttgttg gaactgcgtt gaatcagcgg
      181 ccctgtcttg cgcccgtcaa taatatggta ggccgcatcc cgaaactcga tataagcgtg
      241 ataacgcgag gcgcgcggat cgtccagcac gacgctatta tcaatcgcgc gaccgatggt
      301 gcagataccc tgcggcgaca gagggtattg ccactgacga ccgctcgcat ctttaatctt
      361 gagtaaagcc ataagccaaa tgttcaaacg cgcgacgagg gagatgggag agcgtgacgg
      421 gacgactggc gcaactctat tgctaagcga aaacggctcg cttcacacat tgtagcggaa
      481 gtgcgcctgt gtcaggcaga aattcattga gcgttagctg tcatctccgc cacgtttgcc
      541 gaagggcagg gtcttggtga ggtctttgag cgcggccagt ttcgtgttgt agtgcgggtc
      601 gaggtgcgag agaagactga gcgcagccca gatgtaagag ccgaccgtta aataagtaag
      661 caacggctcg gccaccagtt gcagacgcct gaccagcagc agcagcagga taggcacggc
      721 ggctgtgaac agcgcggtga cgactgacac gacccgcgcc cgcaagccct ccttgcgcga
      781 caacttctcg gcagtcacgc ggtcgaactt gccccactcg gacgccagtg ctgcgatgaa
      841 gtacttgacg atccgagcct tgaattcctc gcgcgtgttt ctggttggcg cgaagaccca
      901 tttgatcgtc gcgcgcaccc cgtttgccat ctccgaggcc gtgagagtcg cccacctgtc
      961 catctccggc acgcctgtga tgaagtagcg gggaaagtgg ttttccatgc aggaagctat
     1021 cgcctccaaa cgctctgcca gccacttccg gtgcgtgaac tcgctccata ttaactcgta
     1081 gccgggttct tcgtgtctgt gcaggatgtt gagcaactca tcaatgacga acgcgcgtgt
     1141 ctggacggct tgcttacggc tttcgagcga tctgaagaag aggcgtccgg gcacgctcag
     1201 gacaaatatg gttggcagga agaacccggt ctgaagcgta aagtagaaga atggccgttg
     1261 acgaaacaaa ggaggctggg cagccatccc gccagtgtaa agcgcgtata aatagccgct
     1321 ggcgacgagg agcaagaacg acgcccacaa tgccatgagc cgatcaagat tcaaaaaatt
     1381 ctccactttt gctccggctc ccgtcgttac aaggaacggc acaaaaatga tcgcgaaggc
     1441 caacggggcg acgagcaggc tccacaaaaa ttcgagtgac caattccata ggccgtgtga
     1501 gttccaatcg ctaaaggggg gaaagaactc tgcggcgagg ggtggcatga cgaggaagag
     1561 tgaaaggatg atgaggccga tgagaacgcc catgtagaac tgaatcatct cgatccataa
     1621 ctcaacttcg gagagttggc gtgtgttcct gtcaaattct acgaagaggc tgctcttgac
     1681 atcaggagag acggatgtga cctgctcgta cttctccagc cgcacctcgc gcaaagactc
     1741 ctgccccttc gcgatccggc tgtgaaacgc cttcgtgtgt gggacacgac tgatgaccgg
     1801 atttaacgcc cgccagaaac gattaggcgg aggaggctgt ggagtgacct cctccgtttc
     1861 ctgcggcgac tgtggcgtct gtgcatcctg cgcatcatcg ctctgttcgg ccatgacggt
     1921 agctctcctg ttatgtttgt gacttgatgg cgtcagataa gccagccaca ctggtcggcg
     1981 gcataacgga cgcgcgccgg gtggtttggc ctaagttccc ttaaaacgtg cggcgtaatg
     2041 tgagtctcac gagcgaagaa ctcattggcg gaatagcgtg tggaagatat aagggaggag
     2101 cggcggaagt caatagtcag cccgccgcgc gggaacggtg agggggcacg ggcgcgcggc
     2161 ggtgttctgc tggtgtgcgc tacacgttga agcggaagtt gaccacgtcg ccttcctgca
     2221 tgacgtactc tttgccttcg aggcgcagca agccctgctc gcgcgcggcg gcgtaagagc
     2281 ctgcgcgtgt gagatcgtcg tagctgacga tctcggcgcg gatgaagccg cgctcgatgt
     2341 cggagtggat cgttccggcg gcttgcgggg cgcgcgtgcc gcgcgggatc gtccacgcgc
     2401 gcacctcttt ttcgcccgcc gttaagaacg acatcagacc gagcaggcga tacgcgctct
     2461 tgatcaggtc ggccacgccg ctgccttcga cgccgagcga ttgcaggtag ccctcacgat
     2521 cttcgggcga gagttcgacg agttccgctt cgatctgcgc gcagatgacg accgattccg
     2581 ccgcttcgct tccggccagc gcgcggacgg ctttaacgtg cgcgttctgg tcgggcgcag
     2641 cgagcgtcgc ttcgtcaacg ttggcggcgt agatggtcgg cttcatggtc aggagaaaga
     2701 gggtgcgcgc ggtggcgcgt tcatcgtctg tgagcgggac gaggcgcgcg ggttgccctt
     2761 cggagagcac tggcaggagt ttgtcgatga gcgcgatctc ggcgagcgcg ttcttgtcgc
     2821 cgcccttggc gttcttctgc gcgcgttcgc ggcggcgttc gacggaggcg aggtctgcga
     2881 gcgcgagttc gatctgaatc gtctcgatgt cgcgttcggg attgacagag ccttcgacgt
     2941 ggacgatgtt atcgtcctcg aagcagcgca cgacttgaat gacggcatcc gtctcgcgga
     3001 tgttggcgag gaactggttg ccgagtcctt cgcctttcga cgcgccgcgc actagccccg
     3061 cgatgtccac gaactcgacc gtcgccggaa ctatcgtgtt cgttttgacg atccgtgcga
     3121 gcggcgcgag tcgctcatcg ggcacgggta cgacgccgat gttggggtca atggtggcga
     3181 acgggtagtt ggcggcgagc gcggaagtct gcgccgtgag cgcgttgaag agcgtggact
     3241 tgccgacgtt cggcagcccg acaatacctg cgcggagcat gaaagcacct ctctcgaatt
     3301 agttcacgcg gttgattgcg agatgctttc ttttacaccg tggcgcaggg aaaggcaaaa
     3361 gtgcaggcga caacctcccg tgcctctccc gcgctcgcgg cgcatcctgc gcgctcagga
     3421 cgaccgcccc gcgcgacgac ttgccccacc gcgcaggcag ttatcgcacc agcgcgagtc
     3481 cagccgccgc gctgccgtcg atgaacggtc gcatctcgcc gacgaattta tcgagcagcg
     3541 ggatgctgta gagcacgacc tcggcagact tttcggggaa gccggattcg agtgtgaagg
     3601 tgtagcccgc gaagacatcg ccgctgtcgt ccacgcccca gaagagaaag ttgcggtcgc
     3661 tcaggttgag caggaggcgc atcaggccga cgttgttttt ggctttgtcg gcgttgatgt
     3721 agccgccctt gaaatgcggg tagacgcgaa agccgatggt atcgtccttc gtgacgccga
     3781 cgacgacttc gaggctctcg gcattcctga gtccatcttt caacacgccg acaaaattga
     3841 agggctgctt ttcactctgc tggaacttga tgttgatgcc tcgcttgggg ccggaggttt
     3901 cgagcagttt gcgcagttgt tcgcgcgtag cgatgcgcgc ggcctctttc tgttgcgcgg
     3961 tcggttgctg cgccgggagg gcgacaggag cggctgccga gatgagtagc gcgatcagga
     4021 tcagtcgttt caaagacttc gatacctccg taaggaatgt tgaattacgc gtgtctggcc
     4081 gaagccgact cgcttgtatg ccaaatcgca cgacaagtca aacaactcgg aaagttttcg
     4141 ccgtgaagcg aggggctttg ccaacacgcg ccgcagttcg cgtataatcg cgtgcgccga
     4201 cgccgtttta gtttttcaat ttaacgcgcg cactccttgc gccgttcgcc atattcgcct
     4261 atgtctttca tgctggggtt gcttgccatg gcaagcggcg cgctcgttac ctatctgtac
     4321 gaccgcgacg cgcacttctt cgtgcggctc gcggcgggcg cgtgcacggg cttcgccgcg
     4381 ctcggcctcg ccggattcgt cttcgcttca ttcctcggac tcacaaccgc atcgctcatt
     4441 ctctccgccg ccgtcgtcgc cgcgccgctc acgctgttgt tgaagcgcga ctggcgcagg
     4501 cgcgtgcgct tcgacgtggg cgaagcatgg cgcgatgtgg aagcggcaat cgtcagaccg
     4561 gaggcgagga cgacgggcgt gctcatcctc ttctttgtct ccgcgctcat cttctggcag
     4621 gtctacgagc gcgcgttgtt cgtgcgcggc ggcgaaatcc tgacgggcgt tgacaacaat
     4681 ctcggcgacc tgccgtttca cttgagcatc atcacgggtt ttgtctatgg cgagaatttc
     4741 ccgccggtgc atcccgaata cgcgggcgcg cgtttgacct acccgtttgt ggtggacttc
     4801 gtggcggcga tgttcgtgcg cgcgggcgcg actctggaag gcgcgctctt ctgggaaaat
     4861 ttcgtgctcg cgctgtcgct cgtcgggctg ctgtatcgct tcgcgttgaa gttgacgcgc
     4921 ggcgaccacc tggccgcgct catcacgccc gcgctcgcgc tcctgagcgg cgggttcggc
     4981 tggtggatgt tcgtgcggga atggcgcggc ggaacgctcg gcctcttcga gttgctcaac
     5041 cggctgccgc acgattacac gatcacttcc gacggcgtgt ttcgctgggg caacgcgatc
     5101 acgacgctgc tcgtgccgca acgcgggctg ctgctcggtc tgccgctcgc gctcatcatc
     5161 attacgcaat ggtggctggc gacggaggaa gagggagacg gggggagagg agaagggagc
     5221 aaggaagaag cgggaaagga agaagcggag ccatctatca ggcgaaaggg gaagggcggg
     5281 aagaaagaga agcgcagttc gccgacggcg acgagcgagc cgccgattcc tgcgctcaac
     5341 cttttccgct tgccctttac ccttactccc gccgtgcagc gaatgatcgg cgcgggcgtc
     5401 gtcgcaggat tgttgccgct cgtgcacgcg cacagtttcg tggtcttgat gttgatgggc
     5461 ggatgcctcg cgttactgtt tccgcgctgg cggctctggt tcatcttttt cgccttgacg
     5521 ctcatcatcg ccgcgccgca gatgtggtgg gcgacgcggc agagcgcggc gcaggcgggg
     5581 agtttcttcg gctggcagtt cggttgggat cgcggtgaac agaacgctct gtggttctgg
     5641 ttgaataaca ccggactctt catccccctg ctcatcgcgg cgattctgtg gcgcggtcgc
     5701 gcccccgtcg tctcgaaacg cctgttgctc ttctacctgc cgttcacgct cctgttcgtc
     5761 atctcgaacg cgggaaaaat ctcgccctgg gtgtgggaca atattaaagt tctttactac
     5821 tggtatctcg cttcggtgcc gctcgtcgcg ctgctctgcg cgcgcctgtg gcgtctgcac
     5881 gcggcgacgc gcgtggccgc catcgtgctc ctgctggtgt tgacgctcgc cggtggcctc
     5941 gacgtgtggc gcgtcgtgtc ggaagcatcg gagcagcgtg agttcgaccg cgctggcgtc
     6001 gcgtttgccg agatcgtcaa atctcagacc gctccgcgtt cactgattct tcacgcgccg
     6061 acttataatc acccggttta tttgacggga cgccgcgccc tgatgggcta cgccgggcat
     6121 ctctggtcgc agggcatcaa ctacacggag cgcgaagccg aactccggcg catgtacgcg
     6181 ggtgcgcccg atgccgagag cctgatggcg aagtacggcg tcgagtatgc ggtcgtcagc
     6241 ccgctcgaac gcagcggggt ggcggtcaac gaacaatttt tcagacgctt cacgaaggtt
     6301 ggagagacag gtggctacag cctctacaaa ctcacgcgcc ggtaaaccga aacgacggcc
     6361 aacgaacgtc gcgcaaggca acgagacggg cgcgagcagt gccgccgcgc cgacgacaac
     6421 gacgaccggc agccacgaga cgagcgggga actctctctg cgcgcatggt ggatcgccta
     6481 tgcgtcggtg ctgatcttgg ccgcgctcgt gcgtctctac caactcgaac tccgaccgat
     6541 gcaccacgac gaaggcgtca acggcttctt cctcaacaac ctgatgcgca cgggcatctt
     6601 caactacgac ccgagcaact atcacggccc gacgctctat tatttcaccc tgccgctcgc
     6661 cctgctcgcg caacattttc agatgttcga ctcgtgggtg gtgcgtctcg tccccctcat
     6721 tttcgggatg gcgaccgtgt ggctcgcgct cggcctgcgt cgctacatcg gggcagtcgg
     6781 cgcgctggcc gccgccgcgc tgctcgcctt gtcgcccggc gtcgtcttct tctcgcgcta
     6841 cttcatacac gagatgatgt tcgtcttctt cacgctcgcc gtcgtcgtgg ccgcgcttcg
     6901 tctctacgag acgcgcgggc gggaagaagc gtcgcccgcg aagaggtggc tcgaactgct
     6961 catcctgctc gccgcgcttc cggtcgtcgc gcagtatgca acgcacatcg cgcagtttgc
     7021 cttgaacctc tccggcatat tttccgggac ggccaatttc gctttcaacc tgccgggcta
     7081 tctcgtgatt ttcggcctcg tcgccctgct cgtgctcgtc gtgctttacg aacgggcgcg
     7141 ggcagcctat ctcatactcg gagccgtcgc cgccgcgctg ctgtttgcga cgaaggaaac
     7201 cgcctttatt tccgtgggcg tgctcctcat cgcctgggcg atgacgtggg gttacgcttc
     7261 aatcgccgga cgacaggcat ggctcggcgg gggagaaccc ctgagcgggc gcgcgcgccg
     7321 caagcagggc ttgaagggaa gcgcgacagc gcccgtgagc gatagcgatt cgcgcggact
     7381 tgtggaacgg ttcggcggct ggcggcgtct gtcgctcctg tccgtcgccg cgctcggcat
     7441 cttcttctcc gtcaatattc tgttctactc gtctttcttc tcgaatccga aaggcgtcgc
     7501 ggactcgctc aaagcgttcg acatctggac aaagacgggc aagagcgagt ttcacgccaa
     7561 gccttttctc acctacgtca agtggctctt ggtggaggag tcgcccgtgc tcgcgctctc
     7621 ctctatgggc gcgctcgtcg ccctgtggcg cgtgcgtcgc aatcgcttcg cgctttttgc
     7681 ggcgttgtgg ggcttcgggc tgctcgccgc ctattcgctg atcaactaca agacgccgtg
     7741 gctcgcgctc agcatgctcg tcccgcttac gctcgtgggc ggctacgcga ttaattcgat
     7801 ctacagagcc gacgagaagc cgggactctg gcgtgtcgtg gcgttgtccc tgttctgctt
     7861 cgcgctgttg acgatgttcg agctgttcat cgcgctcgtt cagccgaaga gcctcatgcc
     7921 ctacctcttc atcaactata agacgctgtg gccttgggtg ggcatgaccc tgttgtttgc
     7981 ggggatgggg agctatgcgt tgaaccagag ctataagacg ggcgggacgg gcagggcggg
     8041 acgcgtcttt gcccttgcca cgctcgcgct cgcgctcgtc gtctgcgcct atcaaaccta
     8101 catgatcaac cttgtccact acgacaacga tgcctatatc tacgtctacg cgcacacgaa
     8161 acgctcgtac gtggagttga tcgatgaggt cgagcgcgtc gccgcgcgtg ccgggacgaa
     8221 gttcgagacg cccatcaccg tcaccgcgcc cgaatactgg ccgatgccct ggtacttgcg
     8281 cgactacaag aatgtcggct atcacggtcg cctcacccct gccaacaacg ccgccgcgat
     8341 catcgccagc aaggcgcagc agtccgaggt gctggcggca aacggcacgc gttacctcct
     8401 cgtgggagat tatgagatgc gccccggcgt catgctcacg ctctacgtcc gccgcgacct
     8461 cgcgggacga tagcaagtgt aagaatgaac gatgaaggcg gaaggatgaa ggatcaatga
     8521 aagcactcaa tcgagttaat gctttcattc atccctcatc cctcatccct ca
//
LOCUS       DASWNS010000059        16694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_210309, whole genome shotgun sequence.
ACCESSION   DASWNS010000059 DASWNS010000000
VERSION     DASWNS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16694
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210309"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VGB76_02125"
     CDS             complement(<1..782)
                     /locus_tag="VGB76_02125"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY0097725.1"
                     /translation="MKKLGFIRQAAALVAVLCAVTGVTTNAQTQTLDAAQTVEAESRG
                     RSRFVISARAGAVNLVAGRAILKKRGDSSELPLTTSVDLEAGDVVETGQNGAVEILLA
                     PGSYFRVGASSTVQMLDTSLERVRLRVENGRAIAEITADSKDSSTEIQTPHGNIVLTK
                     KGLYRVNVEREATALLVRKGEVRVGGAEGQIKVKKNEVLTLSTSGGLLAQNKSSFDKN
                     AQDGFDTWSRERAETLVALNRRLGDGRVETALNSYLSSDRRWS"
     gene            complement(963..2393)
                     /locus_tag="VGB76_02130"
     CDS             complement(963..2393)
                     /locus_tag="VGB76_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEY0097726.1"
                     /translation="MKHRLKSTLKSTLLFLCCAVTLAATLCPPAAAQTRRPNIIFIIT
                     DDQRWDSLGVTGHPFAKTPHLDRVAREGALFNNFFTTTPLCSPSRASFLTGQYAHAHR
                     ITNNDRLGLDVISHTLMTFPRRLREAGYETAFIGKWHMGLDDSRRPGFDHWISFKGQG
                     IYLDPVVNVNGRERQLDGHMTDWLNRWALEFVNRPHPKPFMLYLSHKALHTPYLPAKR
                     HESRYGDAKYIPPASAVDDLSGKPVLRRMVEPVDWARLEGVAPEPPESRRGRGTDRDS
                     IVRDQLRSLAAVDEGVGMLLRALQRKRQLDNTVIVFTGDNGYLLGEHGQFDNKRFAYE
                     ESIRVPFIIRYPKLVRPGMKIDALALNIDVAPTLLSLAGAEPLEKVHGQSLLPLLGNA
                     RTPWRESFLAEYFLEKVAPRAPAWQAVRTAEWKYIHYTELEGMDELYNLRADPHEMRN
                     LLDDPASRPALDRLKAELQRLERETK"
     gene            2700..6182
                     /locus_tag="VGB76_02135"
     CDS             2700..6182
                     /locus_tag="VGB76_02135"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEY0097727.1"
                     /translation="MPLRLKRCLSFLLATSFMLVPAAAQTAKPAPPDLTREPTLYVVG
                     YAHLDTQWRWEYPQVIDEYLPRTMRDNFALFEKYPSYIFNFSGANRYRMMKEYWPEDY
                     ARVKRYVAAGRWFPAGSSMEEGDPLAPSAESLIRQILYGSHYFRREFGRTSAEYMLPD
                     SFGFPASFPSILSHMGLKGFSTQKLYWGSAAEVGGANSPQRTPPGVPFNVGLWEGPDG
                     RGIVSAFNPRPYDSNVREDYSKSPHPGAANPAVNYTPVDWPPRIRRNGEVSGLFTDYT
                     YYGAFGDIGGAPREFSVKLLDAVVGRKMAVLPPPPPALPPADAPGTPIRVGDGEVRVI
                     SATAEQMFLDIKPEWTTRLPRYRGEFLLTNHSAGSINSQTYQKRWNRQNELLADAAER
                     ASVGALWLGARPYPLARLNDAWTLVMGGQFHDILPGTATPKAFEFSWNDDVIALNQFA
                     GVLTSATEGIASGLDTEGQGTPVIVYNPLNIAREDVVEATVSFAGGVPKAVRVFAPDG
                     REVPAQLAGVRDGDARILFLARVPSVGYAVYDVRAADAPAAAPASALKVTGTSLENAR
                     YRLKLDANGDVSSIFDKRLGRELLSAPARLAFQTERPRDWPAWNMDWADRQKPPRTYV
                     GGQVKVRVAENGAARVALEVERETEGSKFVQTIHLSAGDAGNRVEFGNVFDWQSREAA
                     LKAVFPLTAANPRATYNWDVGTIERGNNDERKFEVPSHQWFDLTDRSGGYGVTVLSDC
                     KYGSDKPDDRTLRLTLLYTPGLGAGNAWEYHDQTTQDLGHHEFVYGLAGHGGDWRRDE
                     TDWQAQRLNQPLIAFRSPKHAGALGKSLSLLATNNSRVRVLAFKRAEESDELVVRLVE
                     LDGKRVENVRLKFAAPVVSAREIDGQEQPLGAASISRGELLTSFSPYQLRTFALKLAP
                     SRTRLAAPQSRPVEMPYDLVAASPHGMKPRGGFDRAGRNLPAEMLPAEISYAGVRFRL
                     AGTGAAGKPNALVSRGQTLKLPAGKFTRLYLLAASADGDQRATFRAGGSPFELTVQHW
                     GGFIGQWDTRQWEQGERQVPHGASAEVAARIREPQFRTDPYARMTGIRRGFIKRADLA
                     WYASHHHTPDGVSVPYAYSYLFAYEINLPPGARTLTLPDNDQIRILAVTVSNQREGAR
                     PARPLYDTLEQNAR"
     gene            complement(6353..7771)
                     /locus_tag="VGB76_02140"
     CDS             complement(6353..7771)
                     /locus_tag="VGB76_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0097728.1"
                     /translation="MVQRYGKLLAAVLIFNLSLISASQSIVAQSARAAEQKLSRELVA
                     RLPADLRDATRSHLRLTVEEQTRWLALAPEQLRSTLLARLVERPGAADFLLRLLPREP
                     SARNRVALARAVLNVPHFRTNRRARPALTNLVLSDPDAAVVQQSLDALRGFHLSPLRN
                     LLEQRLEAARARGDRELLRALASEHERWLSLERGTALPAFMRASPPPFSLKRAGQPVR
                     LVAFGDFGQGTEAQKRVAAAMLREHRARPFDFGITTGDNFYPRGMESPTDPRWKSWWE
                     EMYTPLGIRFYAVLGNHDWINFDSPAAEIAYSARSESWRLPAPYYTFTAGAAQFFAVD
                     TDEMSQAQVMWLQDELERSRATWKVVYGHHPVFVSGLSGASYTAEMRRMLWPIIEGRA
                     DLYVSGHHHSLQHLRAPGGGHFVTSGGGGAGTYAVDEREPLALFARSANGFTTIEVGE
                     RELTFKHISDKGDELYAYTIRK"
     gene            complement(7946..8458)
                     /locus_tag="VGB76_02145"
     CDS             complement(7946..8458)
                     /locus_tag="VGB76_02145"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HEY0097729.1"
                     /translation="MPAGMALAATWNRALIEGYERAGTQPLYAFGHGLSYTTFKYGAL
                     GINPSSESASGGREAAPKFEVSFDVTNTGARDGVEVAQIYVGERRPSVPRPAKELKGF
                     AKISLRPGETGRVTIVLDGRAFSYYDVTAKRWRAQPGEFEVSVGRSSAQIELRGRLLL
                     PAKGMAAAGR"
     gene            8445..8630
                     /locus_tag="VGB76_02150"
     CDS             8445..8630
                     /locus_tag="VGB76_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097730.1"
                     /translation="MPAGIAVAGQAKPLNAANEEVVNAANQVDLLLQSHLAENGLDAP
                     LVAGSRRLPRRRSRDQK"
     gene            8705..9325
                     /locus_tag="VGB76_02155"
     CDS             8705..9325
                     /locus_tag="VGB76_02155"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY0097731.1"
                     /translation="MLDKSVLEQLCETADSLFARGYAHGSTGNVSIRLGDEVWITPTG
                     QRLGGLAPARLARLDLGGNLLNENRPSKEHPFHTAIYRQRAEARAVVHLHSTYAVALS
                     CLASFAGEDDLAPLTPYYFMRVAPLAVLPYFKPGSPDLARAIEGVAAAHHSLLLRNHG
                     LICAGATLAEACDRAEELEQTARLHFILRGEEVRTLSPDEVDALRS"
     gene            complement(9420..10433)
                     /locus_tag="VGB76_02160"
     CDS             complement(9420..10433)
                     /locus_tag="VGB76_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097732.1"
                     /translation="MSFDYQKFLPPAVLERVTGLRVSDPELPLRVAHERKRRGRLAPK
                     GKLNILASDHPARRVVRVGDNPFRMANRQEYLARTLRVLQSELVDGVLATMDIIEDLL
                     VIHELMKRAGGAPFLDDKLLIASLNRAGLLGSSWEIDDPMSGPSPAACAAWGLDGTKV
                     LLRLCDAEPDSLKTLLATVRAISEQNALRLPMFLESLPVEKVEGGYRIIKTAEELTRT
                     AGAASALGDSSRYLWLKLPYCENYEMVARSTTLPILLLGGDPVGDVRPFLGELAAGLK
                     AGANVRGALVGRNVLFPGDGDPLAAADAAGGLVHHGWSLEQALDALDTRAGGGLDWLA
                     QLA"
     gene            complement(10437..11474)
                     /gene="iolC"
                     /locus_tag="VGB76_02165"
     CDS             complement(10437..11474)
                     /gene="iolC"
                     /locus_tag="VGB76_02165"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR04382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEY0097733.1"
                     /translation="MMTDAAYDVLAMGRSSIDLYANEIGAPFAEVKSFSAYVGGSPTN
                     ICVGARRLGLRTALLTAVGEDLVGDFVLDFLRREEIAVEFSPRKAGRRTSAFMLAIQP
                     PDKFSIVPYRDNCADLELTTDDVRGAPVEKSRALLLTGSGLSRQPSRDATIYAAETAR
                     ASGKRVVLDLDVRAELWTNMRAYAEGIRPILSLTDVIIGTVEEVAAAAAREGEAAQSP
                     VESPVGSPVESPEVFAGALGEGVRELLAAGSADALVVKRGARSTFVFLRNGETVEAAT
                     FPVEVLNVLGAGDAFAGGFLYGYLKGWDWHRSARMGNACGAIVVTRHGCANFMPYEEE
                     ALAFIQERGGF"
     gene            complement(11471..12787)
                     /locus_tag="VGB76_02170"
     CDS             complement(11471..12787)
                     /locus_tag="VGB76_02170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0097734.1"
                     /translation="MQMNPPATDALATGLAAQAPASGVRYKVLAFLFALAALTYLDRL
                     AISVAAPSIRQEFNLSPVQMGYIFSAFTLTYALFEIPTGWMGDRFGTRRTLTRIVLWW
                     SAFTALTSAAVGFWSLFVMRLLFGMGEAGAIPNSACTVSRWFPASQRGRAMGGVCIGH
                     ALGAALTPPIVFILIQQQGWRLTFIEFGVLGVAWCLAWYWWFRDSPEEHPAVNPAEVK
                     LICAGEERRPDRSHAIPWRTFLRSRNIFFLCAMYFAYGYSLYFYITWLPTYLLEARGF
                     TMTSAGLFAALPWVFGAVAFLCGGTLTDWLVTRTGNKKIARCGVGAFGLTASALMLVL
                     VALTANAVAAALFIALALFFQFLTTPAVWATCMDIGGHRAGVVSGTTNTFGNLAGTLA
                     PIVFGYILQELHSWTLGFYVAAGFLLIGVVMWLFIDPRQTLDEQAA"
     gene            complement(12839..16438)
                     /locus_tag="VGB76_02175"
     CDS             complement(12839..16438)
                     /locus_tag="VGB76_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEY0097735.1"
                     /translation="MIRRYAVRAILLSLILSANALAQGERTLWRIGAFDNSAEEFAAS
                     AGSAPVVYRVADGQPQNWVGTQQAVVPGKAGAGVAPARRIQFELQSAPEGVYRLRLGL
                     ILKTARAPVVELDVNGHRGWFHQPLETYKEGNSEGAILPQYSIGTLTIEVPREFLRAG
                     RNEFALTAVTDELSTAMPGGETTDYAVLTYDALELLHERAAAQASASGVTGARATPTV
                     FFRRVGGRLSEVVSVVVSWRGLVPEGTVTLRLPGGAQSQPLAARGRDFGEQRLEFSVP
                     EFAADTRATVNVRADGREHAFAQTLTPARKWTVYMVPHEHLDVGYSDFQTKLAELHSR
                     IINEALDMNAAHPEFRFTLDGYWQARHFLQGRSEEERQKFYRAVRERKIIVPAQHSVI
                     LTGFPTGEALIRSFYGARRLNREAGGAWDTANITDVPSYSWSYASILAASGLKYFAAA
                     ANADRGPTLMLGDLHRRSPFWWEGPDGARVLMWYSRHYHQIGSEFGLPTHVANGYEGL
                     PSFLGVYERPDYTARAVMLHGSQWENTSLYPQQAALVKQWNSLFAYPELRFSGFGEAM
                     GKISAEAERNGGKLPVVRGDGGPYWEDGIASDAYHAAMERETERRAASAEKLGVIASL
                     VDPRYRPSRDALDCMWENISLMHEHTWGWGRSITEPHSEDSTRELATKRLYGTGARHC
                     AEYLLERGMTAIAGRISTSPRALVVFNTLNWPRDGWVEFDLQKTRELFDLETREVVRF
                     EVLEDHPAYQRIRFRARGVPAVGYKSYDVRDKTAPQSSATNAAASPAATQEAAPGGAT
                     HSGKDTIENSFYRVTLDPKSGGVRSIYDKSLRRELVDQSAAYRFNQYVYVTGGDNPPT
                     FTQLLTYRRHLPLARLEAHAAREGRLVFVGRTPTGISARLESRGVNTPKITTEIILFD
                     NEKRIEFVNRVSKETIYKKEAAYFAFPLALRAPEFKYEVQNGVVRPDRDMMPGAGLEW
                     FSAQNWVSAGEAGAEVSLVSTDSFLWTFGDIVRGTWPTEFKPKGSTVFSYVLNNYWGT
                     NYIAAQGGDFTFRYALTSAPKLDAAAMSRLGWEQTTPLERTLVKSQDKAAPAPDPLPA
                     SRMSFLETSDPSVLLSAWKHAEEGEGMVLRFIELGGAQGARQVRVSSPLFGAFDRRAC
                     NAVEDCAQASAGGAGSFNFETRPRQILTFKLASAQRRTTTTTKTKTRRARARE"
BASE COUNT         2938 a         5502 c         5307 g         2947 t
ORIGIN      
        1 gaccagcgac gatcactgct caagtaactg ttgagcgcgg tttcaaccct gccgtcgccc
       61 aaacgccggt tgagcgccac gagagtctca gcccgctcgc ggctccacgt gtcgaaccca
      121 tcctgcgcat tcttatcgaa ggaagattta ttctgcgcga ggaggccgcc ggaagtgctc
      181 aaggttaaga cttcattctt cttaaccttg atctggcctt ccgctccccc gacgcgcact
      241 tcacctttgc gaaccagcag cgcggtcgct tcccgctcga cgttgacgcg gtagagtccc
      301 ttcttggtca ggacgatgtt gccgtgcggc gtctgtatct ccgtcgaaga atccttcgag
      361 tcggcggtaa tttcggcgat ggcgcggcca ttctccaccc gcaggcggac gcgttcgaga
      421 gaggtgtcca acatctgcac cgtcgaactc gcgccgacgc gaaaatacga tcccggcgcg
      481 agtagtattt cgaccgcgcc gttctgtccc gtctcaacca cgtcgcccgc ttcgagatcg
      541 acgctcgtgg tcaacggcag ttcgctgctg tcgccgcgct tcttcaatat cgcccgaccg
      601 gcgaccagat tcaccgcccc ggcgcgcgcc gagataacga aacggctccg ccctctcgac
      661 tccgcctcga cagtttgagc ggcatccaac gtctgagtct gggcgttggt tgtcacgccc
      721 gtgacagcac agagcacggc cacaagcgcg gcggcctgcc tgataaaacc taattttttc
      781 ataacaattt atcctgcgtt acgaccaatg attgttttcc gtcggttata gaagggagac
      841 tttcagcaat gatatgcctt cgcctgttta aaatcaatca cggcaaaatt tcgcaccact
      901 atcctgtcct gagcctcgtc gggagttgca tgtcgccctg catggggcgt gcccggcgac
      961 ggttacttcg tctcacgttc cagcctttga agttcggcct tgaggcgatc aagcgcggga
     1021 cgcgaggcgg gatcgtcaag gaggtttcgc atctcgtgcg ggtcagcccg caggttataa
     1081 agttcgtcca tcccttccag ttcggtgtag tggatatatt tccactccgc cgtccgtacg
     1141 gcctgccagg ccggagcgcg cggggcgacc ttctccagaa aatactctgc gaggaatgat
     1201 tcgcgccagg gcgtacgcgc gttaccgagc agaggcagca gcgattggcc gtgaactttt
     1261 tcgagcggct ctgcgcccgc cagcgagaga agcgtgggcg cgacatcgat gttcagagcg
     1321 agggcgtcga tcttcatccc cggcctcacg agtttgggat agcggatgat gaagggcacg
     1381 cgtatggact cttcataggc gaaacgcttg ttgtcgaact gcccgtgctc gccgagcagg
     1441 tagccgttat cgcccgtaaa gacgatgact gtgttgtcta gttgtcgctt ccgctgcaac
     1501 gcgcgcaaca gcatcccgac accttcgtct acggcggcga gactacgcag ttgatctctc
     1561 acgattgagt cgcggtctgt gccgcgcccg cgtctggatt cgggcggttc cggggcgacg
     1621 ccttccagcc gcgcccagtc tacgggttca accatccgcc gcaggacggg cttgcccgat
     1681 agatcatcaa cggcggaagc gggcgggatg tacttcgcgt cgccgtagcg cgattcgtgg
     1741 cgtttcgcgg gcaggtacgg ggtgtgcagg gctttgtgcg agaggtagag catgaagggc
     1801 ttcgggtgcg ggcggttaac gaactctagc gcccagcggt tgagccagtc ggtcatgtgg
     1861 ccgtcgagtt gccgctcgcg cccgttgacg ttcaccaccg ggtcgaggta gatgccctgc
     1921 cctttgaagc tgatccagtg gtcgaagccc ggtcgccgtg aatcgtccag ccccatgtgc
     1981 catttgccga tgaaggcggt ttcgtacccg gcctcgcgca ggcggcgcgg aaacgtcatc
     2041 aaggtatggc tgatcacgtc cagaccgaga cggtcgttgt tggtgatgcg gtgcgcgtgc
     2101 gcgtattgcc ctgtgagaaa actggcgcgg ctcggcgagc agagcggggt ggtcgtgaag
     2161 aagttattga agagcgcgcc ttctctcgcc acgcggtcga ggtgcggcgt tttggcgaac
     2221 gggtgccccg tcacgccgag cgaatcccaa cgctggtcgt cggtgatgat gaagatgatg
     2281 ttcggtcggc gcgtctgtgc ggcggcaggc gggcagagcg tggcggccag cgtcaccgcg
     2341 cagcagagaa agagcagagt tgatttcaga gttgatttca gccggtgctt cacacgaact
     2401 cctttcgacc tcgacagagg ctctgttagt tcactcgctt gacgaaaaac cactccgggc
     2461 ttgtgggtca actcatggag agttgcctgt agcctgcggc ctgcgccggg taccagtaga
     2521 aattttcgga cagcaattgg tacgggtcat taagaattgc cggaagaacg taggaaggga
     2581 ttatacttgg gaagctattc ccgcaacacc gaatccgagg ccgacgcgca tcatttcttc
     2641 cttcagccac aacctgacgg gcgtttatcc cggccacaaa atcaaggaga gttcggctca
     2701 tgccccttcg cctcaaacga tgtctatcgt ttctactggc tacctcgttc atgctcgttc
     2761 ccgctgcggc tcaaaccgcg aagcccgcgc cgcccgacct gaccagagag ccgacgctct
     2821 acgtggtcgg ttatgcacac ctcgacacgc agtggcgttg ggagtatccg caggtcatcg
     2881 acgagtacct gccgcgcacc atgcgcgaca acttcgcgct gttcgagaag tatccgagtt
     2941 acattttcaa cttcagcggg gctaaccgct accgcatgat gaaagagtat tggccggagg
     3001 attacgcgcg cgtcaagcgt tacgtcgcgg cgggacgctg gtttcccgca ggctcttcga
     3061 tggaggaggg cgacccgctc gccccttccg ccgaatcgct catccggcag atactctacg
     3121 gctcgcacta cttccggcgc gagttcgggc gcaccagcgc ggaatacatg ctgcccgaca
     3181 gcttcggctt ccccgcttcc ttcccctcca tactttcgca catgggactc aaaggcttct
     3241 ccacgcagaa actctactgg ggttcggcgg cagaggtcgg cggcgcgaac tcgccccaac
     3301 gcacgccccc cggagtccct ttcaacgtcg gcctctggga aggaccagac gggcgcggca
     3361 tcgtctcggc cttcaacccg cgcccctacg acagcaacgt gcgcgaggac tatagcaaga
     3421 gtccgcatcc cggtgcggcg aatccggcgg tgaactacac gcccgtcgat tggccgccgc
     3481 gcatccgccg caacggcgag gtgagcggcc tcttcaccga ctacacctac tacggcgcgt
     3541 tcggcgacat cggcggtgcg ccgcgcgagt tttccgtgaa gctgctggac gcggtagtgg
     3601 gcaggaagat ggccgtcctc ccgccgcctc cgcccgcctt gccgcccgcc gatgcacccg
     3661 gcacgcccat ccgcgtgggc gacggcgaag tgcgcgtcat ctccgccacc gccgaacaga
     3721 tgtttctcga catcaagcct gaatggacta cacgcctccc gcgctaccgg ggcgagtttt
     3781 tgttgaccaa ccactccgcc ggttccatca actcgcagac ctaccagaaa cgctggaacc
     3841 gccagaacga actgctcgca gacgccgccg aacgcgcatc ggtcggtgct ctctggctcg
     3901 gcgcgcgtcc ctacccgctc gcgcgcctga acgatgcatg gacgctcgtg atgggcggcc
     3961 agttccatga catcctgccc ggcaccgcga cgccgaaagc gttcgagttc tcctggaacg
     4021 acgacgtgat cgctttgaac cagttcgccg gagtgttgac gagcgcgacg gagggcatcg
     4081 cctccggcct cgacaccgaa ggtcagggca cgcccgtcat cgtctacaac cctctgaaca
     4141 tcgcgcgcga agacgtggtt gaagcaacag tctcattcgc gggcggcgtg ccgaaagccg
     4201 tacgcgtctt tgcgcccgac gggagagagg ttcccgcgca actcgcaggc gtgcgcgatg
     4261 gcgacgcgcg gattttattc ctcgcccgtg tgccctccgt cggctacgcc gtctacgacg
     4321 tgcgcgccgc agacgctccg gctgcggccc cggcttccgc gctcaaagtc accggcacgt
     4381 ctttggagaa cgcgcgctat cgcctgaagc tggacgctaa cggcgacgtg tcgagcatct
     4441 tcgacaagcg actcgggcgc gagttgctct ccgctccggc gcggctggcc ttccagaccg
     4501 agcgaccgcg cgactggcct gcgtggaaca tggactgggc tgaccggcag aagccgccgc
     4561 gcacgtacgt cggcgggcag gtcaaagtgc gcgtggcgga gaacggcgcg gcgcgcgtcg
     4621 cgctcgaagt cgaacgcgag acggaggggt cgaagttcgt gcagaccata cacctgtctg
     4681 cgggcgatgc gggcaaccgc gtcgagttcg ggaacgtctt cgactggcag agccgcgagg
     4741 ccgcgctcaa ggccgtcttc cccctgaccg ccgcgaaccc gcgcgcgacc tacaactggg
     4801 acgtggggac aatcgagcgc ggcaacaacg acgagcgcaa gtttgaagtg ccgtcgcacc
     4861 agtggttcga tctcacggac aggagcggcg gctacggcgt cacagtgctc tccgattgca
     4921 agtacggctc cgacaagccg gacgaccgga cgctgcgcct gacgctgctc tacacgcccg
     4981 gcctcggcgc gggcaacgca tgggaatacc acgaccagac cacgcaggac ctcggacatc
     5041 acgagttcgt ctacggcctc gccggtcacg gcggcgactg gcggcgcgac gaaaccgatt
     5101 ggcaggcgca acgcctcaat caaccgctca tcgccttccg cagtccgaag cacgcgggcg
     5161 cgctcggcaa gagcctctcg ctgctcgcca cgaacaacag ccgcgtgcgc gtgctggcct
     5221 tcaagcgcgc ggaggagagc gacgaactcg tcgtccgtct cgtggaactc gacggcaagc
     5281 gagttgagaa cgtccgtctc aagttcgccg cgcccgtcgt gtcggcgcgc gagattgacg
     5341 ggcaggagca acctctcggc gcggccagca tctcgcgcgg cgaactgttg acgagtttca
     5401 gtccctatca acttcgcacc ttcgcgctca agctcgcgcc ctcccgcaca cgtctggccg
     5461 cgccgcagtc gcgcccggtc gagatgcctt acgatctggt cgcggccagc ccgcacggca
     5521 tgaagccgcg cggcggtttc gaccgcgcgg ggcgcaatct ccccgcagag atgctgcccg
     5581 ccgaaatctc ctacgcggga gtccgcttcc gcctcgccgg aacgggagcg gcgggcaagc
     5641 cgaacgcact cgtctcgcgc gggcagacat tgaaactgcc cgcgggtaag tttacgcggc
     5701 tctacctgct ggcggcttcg gcggacggcg accagcgcgc caccttccgc gcgggcggca
     5761 gccccttcga gttgacggtg cagcattggg gcggcttcat cgggcagtgg gacacgcggc
     5821 agtgggagca gggtgagcgg caggttccgc acggcgcgtc ggcagaagtg gcggcgcgca
     5881 tcagggaacc gcagtttcgc acagaccctt acgcgcgcat gaccggcatc aggcgcggct
     5941 tcatcaagcg cgcagactta gcctggtacg cctcgcacca tcacacgccg gacggcgtca
     6001 gcgtacccta cgcatactct tacctcttcg cttacgagat caatctgccg cccggcgcgc
     6061 ggacgctgac gctgcccgac aacgatcaga ttcgcattct ggccgtcacc gtctcgaacc
     6121 agagagaggg cgcgcgacct gcacgcccgc tctacgacac gctcgaacag aatgctcgtt
     6181 gacgacagca tctcagcgcg ccctcacaac gtcttaagtt gaagcaaaag gcgcgaaggg
     6241 ttgagcgagc gcacgcggtt ttcaaataaa cttcagggcg tcaggctcaa gtcgtcgcgc
     6301 tcaagtcgtc cggctcaagt cgccgggctt gggacgaata agaacgggcg gcttacttac
     6361 ggatcgtgta agcgtaaagc tcatcccctt tgtcgctgat gtgcttgaag gtcaactcgc
     6421 gctcgccgac ctcaatcgtc gtgaagccgt tggcggagcg tgcgaacagc gcgagcggtt
     6481 cgcgttcgtc aacggcgtaa gtgcctgcgc cgccgccgcc cgaggtcacg aagtggccgc
     6541 cgccgggcgc gcgcagatgt tggaggctgt gatgatggcc gctcacgtac aggtctgcgc
     6601 gcccctcgat gatcggccac agcattctcc gcatctctgc cgtataggac gccccgctca
     6661 gacccgacac gaaaacaggg tgatggccgt acaccacctt ccacgtcgca cggctgcgct
     6721 ccaactcgtc ctgcaaccac atgacctgcg cctgcgacat ctcatccgta tcgactgcga
     6781 agaactgcgc ggctccggcg gtgaacgtgt aatagggcgc gggcagccgc cagctttcac
     6841 tccgcgcgga ataggcgatc tcggcggcgg ggctgtcgaa gttgatccag tcgtggttgc
     6901 cgagcacggc gtagaagcgt atgccgagcg gcgtgtacat ctcctcccac caactcttcc
     6961 aacgcgggtc tgtcggactc tccatgccgc gcgggtagaa gttgtcgccc gtcgtgatgc
     7021 cgaagtcgaa ggggcgcgcg cggtgctctc gcagcatcgc cgccgccaca cgcttctgcg
     7081 cttccgtccc ctgcccgaaa tcgccgaagg ctacgagacg aaccggctga cccgcgcgct
     7141 tgagcgaaaa cgggggcggc gacgctcgca tgaaagcggg cagcgccgtc ccgcgttcca
     7201 gactcagcca acgctcgtgt tcggaggcga gcgcgcgcag caactccctg tcgccgcgcg
     7261 cccgcgccgc ttcgagtcgc tgctccaaaa gatttctgag cggcgacagg tggaaaccgc
     7321 gcagcgcgtc gagcgactgc tggaccaccg ccgcgtccgg gtcggacagg acgagatttg
     7381 tgagcgcggg acgcgcgcgg cggttcgtac gaaagtgcgg cacgttgagg acggcgcgtg
     7441 cgagcgcgac gcggttgcgc gcagacggtt cgcgcggcaa caaccggagc aggaagtctg
     7501 ccgcgcccgg tcgttcgacg agccttgcca gcagagtgct gcgcaactgt tcgggcgcga
     7561 gcgcgagcca gcgcgtctgt tcctcaaccg tcaggcgcag gtgcgagcgc gtcgcgtcgc
     7621 gaaggtctgc gggcagcctc gcgacgagtt cacgcgagag cttctgctcc gccgccctcg
     7681 cgctctgtgc gacgatggat tgcgacgcgg agataagcga caggttgaag atgaggacgg
     7741 cggcgagaag ttttccgtat cgttgaacca ttgtgtgaat tgccctctaa actattaagc
     7801 gcgcgtttcg acatccgccg cctctcgtgg ctccggcgtg agccacttct ctagagaaga
     7861 taaatctcag gcaaaagcgc agcggcgaag atgagttgta gttttcaaat atgaactatg
     7921 ttcgcagtct cgatgtgggc gtgctctaac ggcctgccgc cgccatgcct ttcgcgggca
     7981 gcaggagtct gccccgcagt tcgatctgcg cggacgaccg cccgacggac acttcgaact
     8041 cgcccggttg cgcgcgccaa cgcttagcgg tcacgtcata gtatgagaaa gcgcgcccgt
     8101 cgagcacgat tgtcacgcgc cccgtctcgc cgggacgcag agagattttc gcaaagcctt
     8161 tcaactcctt cgccgggcgc ggcacgctcg gcctgcgctc cccgacgtag atttgcgcca
     8221 cctccacgcc gtcacgcgcg cccgtgttcg tcacgtcgaa cgaaacctca aacttcggag
     8281 ccgcctcgcg tccgcccgag gcgctttcgc tgctcggatt gatccccaga gcgccgtact
     8341 tgaaagtcgt gtatgacagc ccgtgaccga acgcgtagag cggttgcgtg cccgcgcgct
     8401 cgtagccctc gatgagcgcg cggttccacg tcgcggccag cgccatgcct gcgggcatcg
     8461 ccgtggcggg gcaggccaag cctctcaatg ccgcgaatga agaagtcgtc aacgccgcca
     8521 atcaggtcga tcttcttctc caaagtcatc tggctgagaa tggcctcgac gcgccgctcg
     8581 tcgccgggag caggcgactg ccccgccgcc ggagccgcga tcagaaatga gagggcgacg
     8641 accgccgcgc aaattttcct catgggatca ccctccggtc tgctatagat atggagtgat
     8701 ggacatgttg gacaagtctg tattagagca actctgcgag acggctgaca gtctcttcgc
     8761 gcgcggttac gcacacggtt cgaccggcaa cgtctccata cgcctcggcg acgaggtatg
     8821 gatcacgccg accggccagc ggctcggcgg cctcgcgccc gcgcgcctcg cgcgcctcga
     8881 tctcggtggc aaccttctca acgaaaatcg cccgtcgaaa gagcacccgt tccacacagc
     8941 catctacagg caaagagcgg aagcgcgcgc cgtcgtccac ctgcattcga cctacgccgt
     9001 cgcgctctcc tgtctcgcct ctttcgcagg cgaggacgat ctcgcgccgc tcacccctta
     9061 ctacttcatg cgcgtcgcgc cgctcgccgt gcttccttat ttcaaacccg gctctcccga
     9121 tctagcccgc gccatcgaag gcgtggccgc cgcgcaccac tcgctgctgc tgcgcaatca
     9181 cggccttatc tgcgccgggg cgacgctcgc cgaagcgtgc gaccgcgccg aagagttgga
     9241 gcagaccgcg cgactccact tcatcctgcg cggcgaagag gtgcgcacgc tctcgcccga
     9301 cgaggtggac gcgctccgca gttgaagccc gccggactct tctccaatgc cccgcccggc
     9361 agtcgcttca ccaatgcgtc cggctgacag tcttgacggt ctcgacagtc tcgacagttt
     9421 caggcgagtt gcgccagcca gtccagcccg ccgcccgcgc gtgtgtcgag ggcgtcgagt
     9481 gcctgttcca gactccagcc gtgatggacc agcccgcccg cagcatcggc agccgccagc
     9541 ggatcgccgt cgccgggaaa gagcacgttg cgtcctacga gcgcgccgcg cacgttcgcc
     9601 ccggccttga gtccggcggc gagttcgccg aggaatggcc gcacgtcgcc aaccgggtcg
     9661 ccgccgagca ggaggatcgg cagggtcgtc gaacgcgcca ccatctcgta attttcgcag
     9721 taggggagtt tcagccagag gtagcggctg ctgtcgccga gcgcggaggc cgcgcccgca
     9781 gtccgcgtca gttcttccgc cgtcttgatg atcctgtagc cgccttcgac tttttcgacg
     9841 ggcagcgatt cgagaaacat cggaagtcgg agcgcgttct gttccgagat ggcgcgcacg
     9901 gtggcgagca gagttttcag cgaatcgggt tcggcgtcgc agaggcgcag cagaactttc
     9961 gtgccgtcca agccccacgc ggcgcaggca gcgggcgacg ggccgctcat cggatcgtcg
    10021 atctcccaac tgctccccag aagtccggcg cggttgagac tcgcgatgag aagtttgtcg
    10081 tcgaggaagg gcgcgccgcc cgcacgcttc atcaactcgt ggatgacgag caaatcttcg
    10141 atgatgtcca tcgtggcgag cacgccgtcc accagttcgc tctgcaagac gcgcagcgtg
    10201 cgcgccagat actcctgccg gttcgccatg cggaacgggt tgtcgccgac gcgcacgacg
    10261 cgccgcgcag ggtgatcgga agccagtatg ttgagcttac ctttgggggc gagccgtccg
    10321 cgtcgctttc gttcgtgcgc cacgcgcaaa ggcagttccg ggtcgctcac gcgcaacccg
    10381 gtcactcgtt ccagcaccgc cgggggaaga aacttctgat agtcgaatga caaactttaa
    10441 aaccctcctc tttcctgtat gaatgctaaa gcctcttctt cgtagggcat gaagttagcg
    10501 cagccgtgac gcgtgacgac gattgcgccg caggcgttcc ccatccgcgc agacctgtgc
    10561 cagtcccagc ctttcagata accgtagagg aagccgcccg cgaaggcgtc gcccgctccc
    10621 agcacgttca gcacctcgac gggaaacgtc gccgcctcaa ccgtctcacc gttgcgcagg
    10681 aagacgaacg tgctccgcgc tccgcgcttg acgacgagcg catccgccga ccccgctgcg
    10741 agtaactctc gaacgccttc ccccagcgcg cccgcgaaaa cctccggcga ctcgaccggc
    10801 gacccgaccg gcgactcgac cggcgactgc gccgcttcgc cctctcgcgc ggcggcagcc
    10861 gctacctctt ccaccgtgcc gatgatgaca tccgtgagcg acaggatcgg gcggatgcct
    10921 tcggcgtagg cgcgcatgtt ggtccagagt tcggcgcgca cgtcgaggtc gagcacgacg
    10981 cgtttgccgg aggcgcgcgc cgtctccgcc gcgtagatgg tcgcgtcgcg gctcggttgg
    11041 cgactgaggc cggaacccgt caagagcagc gcgcggctct tctcgacggg cgcgcctctc
    11101 acatcgtctg tcgtcagttc gaggtcggcg cagttgtctc tgtagggcac gatggaaaat
    11161 ttgtcgggcg gctggatggc gagcatgaag gcgctcgtgc gccgccccgc tttgcgcggg
    11221 ctgaactcga cggcgatctc ttcgcgccgg agaaagtcga gcacgaaatc gccgacgagg
    11281 tcttcgccta cggcggtgag cagcgcggtg cgcaggccga gccgccgcgc gcccacgcag
    11341 atgttcgtcg gcgagccgcc cacgtaggcc gagaaactct tcacttccgc gaagggcgcg
    11401 ccgatctcgt tggcgtagag atcaatcgaa ctgcgcccca tcgctaacac gtcgtaagcc
    11461 gcgtcggtca tcatgcggcc tgctcgtcga gcgtctggcg cgggtctatg aagagccaca
    11521 tcacgacgcc gatgagaagg aatccggcgg cgacgtaaaa gcccagagtc cacgagtgca
    11581 actcttgcag gatgtagccg aagacgatgg gcgcgagcgt gcccgcgagg ttgccgaagg
    11641 tgttcgtcgt gccgctcacg acgcccgcgc ggtggccgcc gatgtccatg caggtcgccc
    11701 acacggcggg cgtcgtcagg aattgaaaga agagcgcaag ggcgatgaag agcgcggcgg
    11761 ctaccgcgtt cgcagtcagc gcgacgagca ccagcatcaa cgcgctcgcc gtcagcccga
    11821 acgcgccgac gccgcaacgc gcgatcttct tgttgccggt gcgcgtcacg agccagtccg
    11881 tgagcgtgcc gccgcacaaa aaggcgaccg ccccgaagac ccacggcagc gcggcgaaga
    11941 gtcccgccga ggtcatcgtg aagccgcgcg cttcgagcag gtaagtgggc agccacgtga
    12001 tgtagaagta gaggctgtag ccgtaggcga aatacatggc gcagaggaag aagatgttgc
    12061 ggctgcgcag gaaagtgcgc caggggatcg cgtgcgaacg gtcggggcgg cgctcctcac
    12121 ccgcgcagat caatttcact tccgccgggt tgaccgccgg gtgctcttcg gggctgtcgc
    12181 ggaaccacca gtaccacgcg agacaccacg cgacgccgag cacgccgaac tcgatgaacg
    12241 tcagccgcca gccctgctgc tggatgagta tgaagacaat cgggggcgtg agtgccgcac
    12301 ccaacgcgtg cccgatgcag acgccgccca tcgcgcgccc gcgctgcgag gccgggaacc
    12361 agcgcgagac tgtgcacgcg ctgttcggga tggctccggc ctcgcccatg ccgaagagca
    12421 ggcgcatcac gaacagcgac cagaagccca ccgccgcgct cgtcagtgcg gtgaaggccg
    12481 accaccagag cacgatgcgc gtcagcgtcc ggcgcgtgcc gaagcggtcg cccatccagc
    12541 cggtcggaat ctcgaagagc gcgtaagtca gcgtgaacgc gctgaagatg tagcccatct
    12601 gcacgggcga gagattgaac tcctgccgga tggagggcgc ggcgacggag atggcgaggc
    12661 ggtcgaggta ggtcagcgcg gccagcgcga acaggaacgc gagcactttg taacgcacgc
    12721 cggaggcggg cgcttgcgcc gccagcccgg tagcgagggc atcggtcgca ggggggttca
    12781 tctgcataac aaagctatcc cttcgaaatc tctctcggaa tcgtaaaact tgcctgcctt
    12841 actcgcgcgc ccgtgcgcgt cttgtctttg tctttgtcgt cgttgtcgta cgcctctgcg
    12901 cggaagccag tttgaaggtc agaatttgac gcgggcgcgt ctcgaagtta aagctgcccg
    12961 cgccgcccgc gctcgcctgc gcgcagtctt cgacggcgtt gcacgcgcgc ctgtcgaacg
    13021 ccccgaagag agggcttgag acgcgcacct gtcgcgcgcc ctgcgcgccg ccgagttcga
    13081 tgaagcgcag caccatccct tcgccctctt ccgcgtgctt ccacgcgctc aagagcacgg
    13141 acggatcgct cgtctccaga aagctcatgc gggaggcggg cagcgggtcg ggggcggggg
    13201 cggccttgtc ctgcgacttg acgagcgtgc gttcgagcgg cgtggtctgc tcccagccca
    13261 agcggctcat ggccgccgcg tcgagtttgg gcgcgctcgt cagggcgtag cggaaggtga
    13321 aatcgccgcc ctgcgccgcg atgtagttcg tgccccagta gttgttcaag acgtaggaga
    13381 agacggtcga gcctttcggc ttgaactcgg tcggccacgt gccgcgcacg atgtcgccga
    13441 aggtccagag gaacgagtcg gtcgagacga gactcacctc cgcgcccgct tcgcccgccg
    13501 acacccagtt ctgcgccgag aaccattcaa gccccgcgcc cggcatcatg tcgcggtcgg
    13561 gacgcaccac cccgttctgc acttcgtact tgaactccgg cgcgcgcagg gcgagcggga
    13621 aggcgaaata ggccgcctcc tttttataga tcgtctcttt gctcacgcgg ttgacgaact
    13681 cgatccgctt ctcgttgtcg aagagaatga tctcggtcgt gatcttcggg gtgttgacgc
    13741 cccggctctc cagccgcgcg ctgatgcccg tcggcgttct cccgacgaag acgaggcggc
    13801 cttcgcgcgc cgcgtgcgct tcgaggcgcg cgaggggcag gtgcctgcga taggtgagca
    13861 actgcgtgaa ggtcggcggg ttgtcgccgc ccgtgacgta gacgtactgg ttgaagcgat
    13921 aagcggctga ctggtctacc aactcgcgcc gcaagctctt gtcgtagatg ctgcgcacgc
    13981 cgccgctctt cgggtcgagc gttacgcggt agaaagagtt ctcgatggtg tccttccccg
    14041 aatgggtcgc gccgccggga gccgcctcct gcgtcgcggc aggtgaagcg gcggcgttcg
    14101 tcgcactcga ctgcggcgcg gtcttgtccc tcacgtcgta actcttgtag ccgacggcgg
    14161 gcacgccgcg cgcacggaag cggatgcgct ggtaggccgg gtgatcttcg agcacttcga
    14221 agcggacgac ctcgcgcgtt tccagatcga acaactcgcg cgtcttttgc aggtcgaact
    14281 ccacccagcc gtcgcgcggc cagttgagcg tgttgaagac gacgagcgcg cgcggcgagg
    14341 tactgatgcg cccggcgatg gccgtcatgc cgcgctccaa caggtactcg gcgcagtggc
    14401 gcgcgcccgt gccgtagagt cgcttggtgg cgagttcgcg cgtcgagtct tcgctgtgcg
    14461 gctcggtgat gctgcgcccc cagccccacg tgtgttcgtg catcagggag atgttctccc
    14521 acatgcaatc gagcgcgtcg cgcgaaggcc ggtagcgcgg atcgacgagc gacgcgatga
    14581 cgcccagctt ctccgccgag gcggcgcgtc gctccgtctc gcgttccatc gccgcgtggt
    14641 atgcgtcgga cgcgatgccg tcttcccagt aggggccgcc gtcgccacgc acgacgggaa
    14701 gtttcccgcc gtttcgttcg gcctcggcgg atattttacc catcgcctcg ccgaagccgg
    14761 agaagcgtag ttcgggatag gcgaacaggc tgttccactg cttgacgagc gcggcctgct
    14821 gcgggtagag cgaagtgttc tcccactggc tgccgtgcag catgacggcg cgcgccgtgt
    14881 agtccgggcg ttcgtagacg ccgaggaaac tcggcagccc ttcgtagccg ttcgccacgt
    14941 gcgtcggcaa accgaactcg cttccgatct gatggtagtg gcgcgagtac cacatcaaca
    15001 cacgcgcgcc gtcggggcct tcccaccaga agggcgagcg gcggtgcaag tcgccgagca
    15061 tgagcgtcgg cccccggtcg gcgttcgccg ccgccgcgaa atacttcaaa cccgaagccg
    15121 cgaggatcga ggcgtaagac caactgtaag agggcacgtc cgtaatgttg gccgtgtccc
    15181 acgcgccgcc cgcttcgcgg ttcagacgac gcgcgccgta gaaggagcgt atgagagcct
    15241 cgcccgtggg gaagccggtc aggatcacgg agtgctgcgc gggcacgatg atctttcgtt
    15301 cgcgcaccgc ccggtagaac ttctgccgct cctcttcgga acgaccctgt aggaaatggc
    15361 gcgcctgcca gtagccgtcg agcgtgaagc gaaactccgg gtgcgccgcg ttcatgtcca
    15421 gagcctcgtt gatgatgcgg ctgtggagtt cggcgagttt ggtttgaaag tcggaatagc
    15481 ccacgtccag atgctcgtgg ggcaccatgt agaccgtcca cttgcgcgcg ggcgtgagcg
    15541 tctgcgcgaa agcgtgttcg cgcccgtcgg cgcggacgtt gacggtggcg cgcgtgtcgg
    15601 ctgcaaactc cgggacggaa aattcgagcc gctgttcgcc gaagtcgcgc ccgcgcgccg
    15661 cgagcggctg cgactgcgcc ccgccgggca accggagcgt gaccgtgcct tcgggcacga
    15721 ggccgcgcca actgacgacg accgagacga cctccgagag ccgcccgccc acgcgccgga
    15781 agaagaccgt cggcgtcgcg cgcgcgcccg tcacgccgga agcagacgcc tgcgccgccg
    15841 cgcgctcgtg caacaactcc agcgcatcgt aggtgagcac ggcatagtcg gtcgtctcgc
    15901 cgccgggcat ggcggtcgag agttcgtcgg tcacagccgt cagagcaaac tcgttccgcc
    15961 ccgcgcgcag gaattcgcgt ggcacctcaa tcgtcagcgt gccgattgag tactgcggca
    16021 ggatcgcccc ctcgctgttc ccttccttgt aggtttcgag cggctggtgg aaccatcccc
    16081 gatggccgtt cacgtccaac tcgacgacgg gcgcgcgcgc cgtcttcagg atcaatccga
    16141 gccgcagacg atagacgccc tccggtgcgg actgtagttc gaactgtatc ctgcgcgccg
    16201 gagcgacgcc cgcccccgcc ttgccgggga cgacggcctg ctgcgtgccc acccagttct
    16261 gcggctgccc gtcggcgact ctatacacaa ccggcgcgct tccggcggaa gcggcaaact
    16321 cttccgcaga gttatcgaac gcgcctattc tccagagcgt gcgctcgccc tgcgccagag
    16381 cgttcgcgga caaaatgagc gacagcaata tcgcccggac ggcgtatcta cgaatcaata
    16441 gcttctctcc acacaattat tctgtagcgc tgtgaattac aacaggctgc gagcttaagt
    16501 ttcgaattgt ccagcgggat tgtggttaga tatgccccgt taagactcag aacaggtttt
    16561 cttacctgtc atggttgctg cacctgcccg tcgctgatag acgcgaaccg gacaacgcga
    16621 tccggtgccc ttactatccg gccagccctt gcgtgtgcag cgtttgaaag aatcgaacgt
    16681 ggcagcaaaa tact
//
LOCUS       DASWNS010000060         7860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_495578, whole genome shotgun sequence.
ACCESSION   DASWNS010000060 DASWNS010000000
VERSION     DASWNS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495578"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VGB76_02180"
     CDS             <1..641
                     /locus_tag="VGB76_02180"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY0097736.1"
                     /translation="IGFGLQFIAGDIASGFILLFERPARIGDFVTITGPDSKLTEGRV
                     QAIKLRTTAVLTNDRITVIVPNSKLVNDNLVNWSYADRRSRISVPVGVSYDSDVDLVT
                     DTLLRAAEGITHVLPDPKPSVQFLGFGESSLDFRLLVWTDRPRRHPSIRSDINYRIWR
                     LFKEAGIEIPFPQRDLNLRGGALRLAAPDGQQFTLSDGGEVDVEHEENLTRS"
     gene            645..818
                     /locus_tag="VGB76_02185"
     CDS             645..818
                     /locus_tag="VGB76_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097737.1"
                     /translation="MKKGLPYCCDAMRSNLDYRCDEHPDPFDGPDNLIYYASFTAPDD
                     TIYLFAFHLFSYF"
     gene            897..1517
                     /locus_tag="VGB76_02190"
     CDS             897..1517
                     /locus_tag="VGB76_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0097738.1"
                     /translation="MRSARSLNHGQRAAVPDKREAILRAATKVFATSGYFNSKVADVA
                     RVAGVADGTVYLYFKSKEEILRSIFERGVSEAIAAARAETAMLDDAREKLRRIARLHL
                     ERLGADRDLAVVFQVELRGSTKFMEEFSAAGLAEYLRLIRETLEEGQRAGLFRAELNA
                     KIVAKILFGALDEMATNWILSPRAYKLAPLADQVLDIFLNGVSNKR"
     gene            1514..3481
                     /locus_tag="VGB76_02195"
     CDS             1514..3481
                     /locus_tag="VGB76_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY0097739.1"
                     /translation="MTPNPNLSNRPGHATETLNTPGASADERAASNSTSAAPPADEFI
                     PIPPPPRVPLAVDEPTTLAEVFALATSRHQKPDALNYKQEGQWRAISSADLRERAHLI
                     AYGLYALGLRAGDRAAILSESCPEWTLTDAGCQFAAVVDVPIYPTQAPPQVRYILDDS
                     GARVLFIRNRAAFERIFAAIDGCDTLEHLIFFEPEGAAEAGASTLDELIARARTLRDE
                     QPALIDDLARAAKPDDLATIIYTSGTTGEPKGVMLTHSNLVSNLIDSSGHLAFSESDI
                     ALSVLPLSHVFERLGMYMYIHHGMSVYYAESIEKIGDNIREVRPTIMLCVPRLFEKIY
                     ARIQEKAAAGGKLKAGMLAWAVNVGKLWAQHVVAGRPVPPLLALKHDLAKRLVFSKWR
                     EGVGGRMRLFVSGGAALPEEIGYIFLGAGLPIVQGYGLTETSPVIAAGNLEENRIGTV
                     GRPIKNVEVRIAADGEIETRGPNVMRGYYRKPAETRAVFTEDGWFKTGDIGLIDPDGF
                     LKITDRKKELFKTSGGKYIAPSVVEARIKSSRFVNQVVLIGNGRKYAAALIVPDWEQL
                     RAYTQHKGIREGVATPAEMCRHERILDLMQRQVDALCADLSNYERVKRIALLEHELSI
                     EGGELTPTLKVKRRVVDEKYSDVINRLYAGS"
     gene            3779..5170
                     /locus_tag="VGB76_02200"
     CDS             3779..5170
                     /locus_tag="VGB76_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY0097740.1"
                     /translation="MSWKKTINLLREAVLGSNQDFTEGSIGRAIALLSVPMILEMMME
                     SLFGIVNVFYVARLGADAIATVGLTESMLTIVFGIAIGLSMATTATVARRTGEKDPEG
                     AAVAAVQSIIVGVLISLPVAVAGVAFAPALLALMGAEASVVATGSGYASIILGGNVCI
                     MLLFLINAIFRGAGDAAVAMRSLWLANLVNIVLDPCFIFGLGPFPELGVTGSAIATTI
                     GRGTGVVYQLWILLGGKSRVQVRREHLRLDAPVMLKLLRVSLGGMFQFLVATASWLGL
                     TRIVAVFGSAALAGYTLSLRILIFAMLPSWGMSNAAATLVGQNLGAGKPHRAERSVLL
                     AGLSNVVFLAIVALIFIAFTEQIIGIFTTDASVVAYGVTSLRLVSYGFIFYGYGMVMV
                     QAFNGAGDTYTPTIINLGCYWLFQIPLAYALAIHFDMGARGVFIAIPIAETLLTVLAI
                     IYFRRGRWKARKL"
     gene            complement(5343..6023)
                     /locus_tag="VGB76_02205"
     CDS             complement(5343..6023)
                     /locus_tag="VGB76_02205"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0097741.1"
                     /translation="MRATLYPVGNLFIPAPHGKLEAILKEPRPAEGGSARGVALVLHP
                     HPLHGGTMHNKVVFRAARALNDAGCVTLRVNFRGVGQTTGTHDHGQGEQADAALALDY
                     LVEHYPRLPLILAGFSFGARVGLEVGISDERVRALVGIGTPVSIPERGYDFSFLRECR
                     KPVLFVHGDRDEFGDVAKLQTLVAELPAATDARVEVIAGAGHFFDEQLDELTRVIREW
                     AERVLEEA"
     gene            complement(6232..>7860)
                     /locus_tag="VGB76_02210"
     CDS             complement(6232..>7860)
                     /locus_tag="VGB76_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0097742.1"
                     /translation="ENLACTAVAPTINGQIAAGDPTQPTRIFRGGTPSTCTNRPFPGT
                     NADPSPVNRRYDQYTFTNTSASPACIRVTFRADNAGIHSVAYLGAYNPANISQNYLGD
                     SGAAYTANTFASYSFTVPAGATYVVVMHEITPNTPAVINYSLAVCDSTGTTTQPAPVA
                     QVGQVLISEFRPSGVTTPAPSPSPAPAPAFSVNDEYVELYNNTDGPIDVGGFAVVSNR
                     DGTDFQLGFPAGIIIPRRGHLIVVNSFPGSGYSLSGYAAADLSIGTLADLWQENQGIA
                     LVDASRTTVIDSVGFVGNGGSLPYIEGNGLRRTGQKPAVQYAYVRKTSPTTNYPQDTG
                     DSASDWLLVSVTGAAFPGQPAASGPVQTILGAPGPENRSSPIERSAFIPPSLVDPTAP
                     ANGGENRRRLGCGAPNTPPCDANTSSSGFLSLRRKFTNNTGANVTRLRFRITDMSTLG
                     SPGDGPGQADLRALTSTTITATINGVPTTIEGTTLEQPPNQPIGGGVNSSLNVGTITF
                     GTPLPSGQSINVQFLLGVQRDGAFRFVINTEALP"
BASE COUNT         1426 a         2465 c         2449 g         1520 t
ORIGIN      
        1 gcatcggctt cggcttgcag ttcatcgcgg gcgacatcgc ctccggcttc atcctgctct
       61 tcgaacgccc cgcgcgcatc ggcgatttcg tcaccatcac cggacctgac agcaaactca
      121 cagaaggccg cgtgcaggcg atcaagctgc gcacgacagc cgtgttgact aacgaccgca
      181 tcaccgtcat cgtgcccaac tcgaaactcg ttaacgacaa tctcgtcaac tggtcttacg
      241 cagaccgccg ctcgcgcatc tccgtgcccg tcggcgtctc ttacgattcc gacgttgatc
      301 tcgtcaccga cacgctcctg cgcgccgccg agggcatcac gcacgtcttg cccgacccga
      361 aaccgagcgt gcagttttta ggcttcggcg aatcgtcgct cgactttcgt ttgctcgtct
      421 ggacggacag gccgcgccgc cacccgtcca tcagaagcga catcaactat cgcatctggc
      481 gtctcttcaa agaggccgga atcgaaattc ccttcccgca gcgcgatctc aatctgcgcg
      541 gcggagcact tcgtctcgcc gcacctgacg gccagcagtt caccctctcg gacgggggcg
      601 aagtggacgt ggaacacgaa gaaaatctga cgcggagtta acttgtgaag aaggggcttc
      661 cgtattgctg cgacgcgatg aggtcgaatc tcgactatcg ttgcgacgag catccagacc
      721 ctttcgatgg tcctgacaat cttatctatt acgcttcatt caccgcaccg gacgacacga
      781 tttatctctt cgcctttcac cttttttctt acttctgata cgctgtcaga gagctactga
      841 atgagcagtc agtcaccgaa gcgtccaagt aagcgaaacg gagttgtgac ccccgaatgc
      901 gttccgcacg ttccctgaac cacggccagc gcgccgctgt gccggacaag cgcgaggcca
      961 tcctgcgcgc cgccacgaaa gtcttcgcca cgagcggtta cttcaactcg aaggtggccg
     1021 acgtggcgcg cgtcgcgggc gtcgccgacg gcacggtcta tctctacttc aaaagcaaag
     1081 aggagattct gcgttcgatc ttcgagcgcg gcgtgagcga ggccatcgcc gccgcacgcg
     1141 cggagacggc catgctcgac gatgcgcgcg agaagttgcg ccgcatcgcg cgcctgcatc
     1201 tggagagact cggcgcggat cgcgatctgg ccgtcgtctt tcaggtcgaa ctgcgcggct
     1261 cgacgaagtt catggaagag ttttccgccg ccgggctggc cgaatacctg cgcttgatcc
     1321 gcgagacgct cgaagaggga cagcgcgccg gactctttcg cgcggaactc aacgccaaga
     1381 tcgtcgccaa gattctcttc ggcgcgctcg acgagatggc gaccaactgg attctctccc
     1441 cgcgcgccta caagctcgcg ccgctggccg atcaagtgct cgacattttc ctgaacggag
     1501 taagcaacaa aagatgaccc ccaatccaaa cctgagcaac agaccaggac acgcgaccga
     1561 gacgctcaac acgcccggcg catccgccga cgagcgagca gcctcaaact ccacttctgc
     1621 cgcgcctccg gcggatgagt tcattcccat tccaccgccg ccgcgtgtgc cgctggcagt
     1681 tgacgaaccc acgacgctcg cagaagtttt cgcgcttgcc acgagccgcc atcagaagcc
     1741 cgacgcgctc aactacaagc aggaaggcca gtggcgcgcg atctcttccg ccgacctgcg
     1801 cgaacgcgcg cacctcatcg cctacggcct ctacgccctc gggttgcgcg cgggcgaccg
     1861 cgccgcgatc ctctccgaga gttgccctga gtggacgctg acggatgcgg gctgccagtt
     1921 tgccgccgtc gttgacgttc cgatctaccc gacgcaagcg ccgccgcagg tgcgttacat
     1981 cctcgacgat tccggcgcgc gcgtgctctt catacgcaac cgcgccgcct tcgaacgcat
     2041 cttcgcagcc attgacggtt gcgacacgct cgaacatctc atcttcttcg agccggaggg
     2101 cgcagccgaa gcgggcgcat cgacgctcga cgaactcatc gcacgcgctc gcacgctgcg
     2161 cgacgagcag cccgctctga ttgacgacct cgcacgcgcc gctaagccgg acgacctcgc
     2221 cacgatcatc tacacttccg gcacgacggg cgagccgaaa ggcgtcatgc tcacgcactc
     2281 gaacctcgtc tcgaatttga tcgatagctc cggccatctc gcgttcagcg aatcggacat
     2341 cgcgctctcc gtgcttccgc tctctcacgt cttcgagcgg ctcgggatgt acatgtacat
     2401 ccatcacggc atgagcgtct attacgcgga gtcaatcgag aagatcggcg acaacatacg
     2461 cgaggtgcgc cccacgatca tgctctgcgt gccgcgcctg tttgaaaaaa tttacgcgcg
     2521 cattcaggag aaggccgccg ctggcggcaa actcaaggcc ggtatgctcg cgtgggcggt
     2581 caacgtgggg aaactctggg cgcagcacgt cgtcgccggg cggccagtcc cgccgcttct
     2641 ggcgttgaaa catgatctcg ccaagcgtct cgtcttttcg aagtggcgtg agggcgtggg
     2701 cgggcggatg cgcctgttcg tttcgggcgg cgcggctctg cccgaagaga tcggctacat
     2761 cttcctcggc gcggggctgc ccatcgtgca gggttacggt ttgacggaaa cctcgcccgt
     2821 gatcgccgcc ggaaatttgg aagagaatcg catcggcacg gtcgggcgac cgatcaaaaa
     2881 cgtcgaagtg cggatcgctg cggacggcga gatcgagacg cgtggaccaa acgtgatgcg
     2941 cggctactac cgcaagcccg ccgagacgcg cgccgtcttc acggaggacg gctggttcaa
     3001 gacgggcgac atcggcctca ttgacccgga cggtttcctg aagatcacgg atcgcaagaa
     3061 agagttgttc aaaacttccg gcggcaagta catcgcgcct tcggtcgtcg aagcgcgcat
     3121 caagagttcg cgcttcgtca atcaagtcgt gctcatcggt aacgggcgca agtatgccgc
     3181 cgcgctcatc gtccccgatt gggagcaact gcgcgcctac acgcaacaca aaggcatccg
     3241 cgaaggcgtg gcgacgcccg ccgaaatgtg caggcacgag cgcatcctcg atctcatgca
     3301 gcgacaggtg gacgcgctct gcgcagacct ctccaactat gaacgcgtca agcgcatcgc
     3361 gctcctcgaa cacgagcttt caatcgaagg cggcgaactc acgcccacgc tcaaggtcaa
     3421 acgccgcgtc gtcgacgaaa aatacagcga cgtgatcaac cgtctctacg cgggcagtta
     3481 aaaaattgaa acctgagcgc ggcgagcatc gttagacaac tttccgtttg gagctggcgg
     3541 caggctattc accgcagaga gcgagatata agcgcagcac gcgcgcagag gaaagagaga
     3601 tgaagagcga acgatgaatg aggaatgacg acggccactc gcctgagtgc tgtgttattc
     3661 gtccttcatc gcttcgcctt catcgttttt tctctgcgcc cgcgctgcgc ttctttttgc
     3721 gcctgcgacg gtgagcgcac gtagttaaag ttaattttaa ccggataaca ggacagttat
     3781 gagttggaaa aagactataa acttgctgcg cgaagctgtg ctcggctcaa atcaggattt
     3841 cacggagggc agcatcgggc gcgccatcgc cctgctctcc gtgccgatga tccttgagat
     3901 gatgatggag tcgctgttcg gcatcgtcaa cgtcttctac gtggcgcggc tcggcgcgga
     3961 tgctatcgca accgtcgggc tgaccgagtc gatgctcacc atcgtcttcg gcattgccat
     4021 cggactgagc atggcgacga ccgcgaccgt cgcgcgtcgc acgggcgaaa aagacccgga
     4081 aggcgcggcg gtggcggcgg tgcaatcaat catcgtcggc gtgctgatct ctttgcccgt
     4141 cgcagtcgcc ggggtggcct ttgcgcccgc gctcctagcg ttgatgggcg cggaagcgag
     4201 cgtcgtggcg acgggttcgg gctacgcctc gatcatcctc ggcggcaacg tctgcatcat
     4261 gctgctgttt ctcatcaacg cgatctttcg cggggcgggc gacgcggcgg tggcgatgcg
     4321 ctcgctctgg ctcgccaacc tcgtcaacat cgttctcgac ccctgtttca tcttcggcct
     4381 cggccccttt cccgaactcg gcgtgaccgg ctcggccatc gcgacgacca tcgggcgcgg
     4441 cacgggcgtc gtctatcaac tctggatttt gctcggaggc aagagccgcg tgcaggtcag
     4501 gcgtgagcat ttacggctcg atgcgccggt gatgttgaag ctgctgcgcg tctctctggg
     4561 cgggatgttt caattcctgg tggcgacggc gagttggctg gggctgacgc gcatcgtcgc
     4621 cgtcttcggc agcgcggctc tggcgggcta cacgctctcg ctgcggattc tcatctttgc
     4681 gatgctgcct tcgtggggca tgtcgaacgc ggcggccacg ctcgtcggtc agaatctcgg
     4741 cgcggggaaa cctcatcgcg cggagcgttc cgtgttgctg gcgggtttga gcaacgtcgt
     4801 cttcctcgcc atagtcgcgc tcatcttcat cgccttcacc gagcagatca tcggcatctt
     4861 tacgacggat gcctctgtcg tcgcttacgg cgtcacgtct ctgcgcctcg tcagctacgg
     4921 cttcatcttc tatggctacg gcatggtgat ggtgcaggcg ttcaacggcg cgggcgacac
     4981 ctacacgccg accatcatca acctcggctg ctactggctc tttcaaatcc cgctcgccta
     5041 tgcgctcgcc atccatttcg acatgggcgc gcgcggagtg ttcatagcga ttcccatcgc
     5101 cgagacgctg ctcaccgtcc tcgccattat ttacttccgg cgcgggcgtt ggaaagcaag
     5161 gaaactctga gacgacggcg gcggcttctc ttcattcaaa gcctcgaact ctcatcctcc
     5221 aaagcgaagg ggcggtcaac tggcgaccgc cccttcgcac cgctcttaga cgacgcctca
     5281 agttcccgcg atagacttcg agggagcgcg tcgtttaccg cttcgacagg ttacgcgtcc
     5341 tcttacgctt cttccagaac tcgctccgcc cattccctga tcacgcgcgt caattcatcg
     5401 agttgttcgt cgaagaagtg tcctgcgccc gcgatgactt cgacgcgtgc gtcggtggcg
     5461 gcgggaagtt cggcgacgag tgtttggagt ttggcgacat cgccgaactc gtcgcggtcg
     5521 ccgtgtacga agaggacagg cttgcggcac tcgcgcagga atgagaaatc gtagccgcgt
     5581 tcggggatgc tgacgggcgt gccgatgccg acgagggcgc gcacgcgctc gtcactgatg
     5641 ccgacttcca gccccacgcg cgccccgaag gagaagcccg cgaggatgag cggcaagcgt
     5701 gggtaatgct ctacgagata gtcgagcgcg agcgcggcgt ccgcctgttc gccctgcccg
     5761 tggtcgtgcg tgccggtcgt ctgcccgacg ccgcgaaagt tgacgcgcag cgtgacgcag
     5821 cccgcgtcgt ttaaagcgcg tgcggcgcgg aagaccactt tgttgtgcat cgtgccgccg
     5881 tgcagcgggt gcgggtgcag cacgagcgcg acgccgcgtg cgctgccgcc ctccgccggg
     5941 cgcggctctt ttaagatcgc ttcgagtttg ccgtgcgggg cgggaatgaa aagattgccg
     6001 accggataca aagttgctct cacctcgttt gtgaattctg ccctgcggac tggcggaaga
     6061 gactagcaga gaagcgtccg ggggcaaacc gtgaggcggc gggagaaaag agcgggggcg
     6121 cgaaagttaa ctttcgcgcc cccggatgat gtgcgtccga actttcagcc ggatggctaa
     6181 aggtttgtcc gcaggttagt gcgacgacct gttaagtcgc agcctcaact tctagggcag
     6241 tgcctccgtg ttgatgacga agcggaacgc gccatcgcgc tgcacgccta agaggaactg
     6301 cacgttgatg ctctgaccac tcggcaacgg tgttccgaac gtgatggtgc cgacgttcag
     6361 actgctgttg acgccgccgc caatcggttg attgggcggt tgctcaaggg tcgtgccctc
     6421 gatggtcgtc ggcacgccgt taatggtggc cgtgatggtc gtcgaagtga gcgcgcgtag
     6481 atcggcctgg ccgggtccgt cgccggggct gccgagcgtg ctcatgtcgg tgatacggaa
     6541 gcgcaagcgt gtgacgttgg cacccgtgtt gttggtgaac ttccgccgca ggctcaggaa
     6601 gccggaggaa gaagtgtttg catcgcacgg cggcgtgtta ggcgcaccgc aaccgagacg
     6661 cctgcggttc tcgccgccgt ttgccggcgc ggtcggatcg acgagcgacg gaggaatgaa
     6721 cgcactgcgc tcaatcgggc ttgacctatt ctccggcccc ggcgcgccga ggatggtttg
     6781 aacaggccca gaagcggcag gctggccggg gaatgccgcc ccggtcacgg aaaccaacag
     6841 ccagtcgctc gcgctgtcgc cggtgtcctg cgggtagttg gtggtcggcg aagtcttacg
     6901 cacgtaggcg tattgcaccg ccggcttttg tcccgtccga cgcaggccgt tgccttcgat
     6961 gtacggcagg cttccgccgt taccgacgaa gccgaccgaa tcgatgaccg tcgttctgct
     7021 ggcatcaacc agagcgatgc cctgattttc ctgccagagg tcggcgagcg tgccgatgct
     7081 caagtcagcc gcggcatagc cgctcaagct gtagccgctg ccggggaacg agttgacgac
     7141 gatcaggtgg ccgcgacgcg ggatgatgat gcccgccggg aagccaagct ggaagtctgt
     7201 gccgtccctg ttactgacga cggcgaagcc gccgacatca atcggtccat cggtgttgtt
     7261 atacaactcg acgtactcgt cgtttacgct gaacgccgga gcgggagccg gactcgggct
     7321 gggagccgga gtggtcacgc ccgacgggcg gaactcgctg atgagcactt gaccgacctg
     7381 cgccacgggc gcgggctgcg tcgtggttcc ggtgctgtcg cagactgcga gcgagtagtt
     7441 gatcacagcc ggagtgttcg gcgtgatctc gtgcatcacg acgacgtagg tagccccggc
     7501 gggaacggtg aacgagtacg aggcgaacgt gttggccgtg taagccgcgc ccgaatcgcc
     7561 gaggtagttc tgcgagatgt tcgcagggtt gtacgcgccc agataggcga cgctgtggat
     7621 gcccgcgttg tcagcccgga aggtgaccct gatacaggcc ggcgacgcgc tcgtgttggt
     7681 gaaggtgtac tggtcgtaac gacgattgac cggcgacgga tcggcattgg ttccggggaa
     7741 cggcctgttc gtgcaggtgc tcggcgtgcc gccgcggaag atgcgggtcg gctgcgtcgg
     7801 gtcgcccgcc gcgatctgcc cgttgatggt cggggcgact gccgtacagg caaggttttc
//
LOCUS       DASWNS010000061         4796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_365672, whole genome shotgun sequence.
ACCESSION   DASWNS010000061 DASWNS010000000
VERSION     DASWNS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365672"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VGB76_02215"
     CDS             <1..769
                     /locus_tag="VGB76_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0097743.1"
                     /translation="DDVAGVIGTHIYGMPCHVDELQRVVADYAQRQSRRVFLLYDAAH
                     AFGSSIRGRRVGSFGDAEVFSLSVTKMLVSVEGGMISSRHPELIGRIRKMRNYGIEAN
                     YDTYWPGMNGKMSEFHAIIGLHNLRRLDELLAERQHKAKYYFTRIHERTGFETLPWPD
                     NVVHTFKDLTVLVPERRIGERDEVVAFLKTQGIETRAYFYPPVHEQRLFQSFADRALP
                     RTESLSRRVITLPFYTAMTEDEMDCVVQALRLYEEGK"
     gene            854..1906
                     /locus_tag="VGB76_02220"
     CDS             854..1906
                     /locus_tag="VGB76_02220"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0097744.1"
                     /translation="MNILVTGGAGYLGSVLIPKLLARGHRVRVLDLGYFGLGHLRALR
                     PSVEVIREDLRALAAAPAQMHALLDGCDVVIHLAAVSNDPSAELNPGLTEEVNFHATI
                     ALAEAARALGVKFLFSSSCSVYGEAEGELDEESWVNPLTAYASSKVKAEQGLSALAQT
                     GWRPVILRNGTLFGYSPRMRFDLVVNIFSLYSTLHNEIKIFGDGQQWRPFLHVSDCAR
                     AFVFFTEQPEQQHLCYNIAHENLRVVDIAARFMQLNPSLQATHVETPDEDRRDYRVST
                     ERIGAEGFRTRTGVEVGAEEMIEAIINGLIPDPESIFYRNAKWLKELTQIGSKNHRDI
                     VSLLETLSHLNLPTRV"
     gene            1946..2620
                     /locus_tag="VGB76_02225"
     CDS             1946..2620
                     /locus_tag="VGB76_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEY0097745.1"
                     /translation="MNPNRTVQVIIVGAGGHGSELHAYLRELCVQGSGIEFLGFIDDH
                     RPRGAWGDTEVLGGLACLEEFLGARVGELHYITAVGSNTLRRTFVERLRRIDEQRLKP
                     WTLQHPRAITGHGVEFGAGTCLAPGSIVTTNARIGSHCILNVNTSVSHDAVVGDYTNL
                     NPGVVVAGNVEIGEGCYIGAGATLIDNVKIGEWSTIGAGAVVIGDIPPRVTAVGVPAR
                     VIKYHE"
     gene            2613..3611
                     /locus_tag="VGB76_02230"
     CDS             2613..3611
                     /locus_tag="VGB76_02230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0097746.1"
                     /translation="MSNMMPLITFIIPCYNYARYLPDCLNSILQQEGKFDFEIILIDD
                     ASTDDTGEVIRSYNDPRIRVITHAVNVGHAGTISEGLKLARGEYVARIDPDDRYRPGY
                     LSRVIPKFEAHPEVGLVYGNVSLIDEHGATSVGRGDRVHGGRDYKGCELVALLGQNFI
                     CAPSVMARREAWLKALPVPEWLAFNDWYFTLMMARQYEFYYLDEVLADYRVHGTNHHS
                     RIVLNKTEEPSIFWLLDKIFSTPETSPELEIAKQRARRRIYGAQYLDMADKYFGCDYC
                     GDARRCYLAALRHHPAYLLNPGVLRRLLATFIGRKTYESSKSTVKAVLAPLARVRR"
     gene            3616..4308
                     /locus_tag="VGB76_02235"
     CDS             3616..4308
                     /locus_tag="VGB76_02235"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0097747.1"
                     /translation="MKITTRIAYSLFPSYVWGTLREELKLTRCRWKARRRVDGHIFAG
                     SGYKLHLGSGPRVVPGWFNIDGAPQDQLDLQWDLRTPLPFANRSCRLIYSEHLLEHLF
                     KEDALNLLQECNRLLEPGGSIRLGVPDAELYLRAYVERRTDFFQGLKHLGGAVIPLST
                     PIDVINQMFRMGGAHLFAWDFETIERALVDAGFTQITRWPSGQASSPELCLDDPEHAF
                     ETLYVEAVKPGV"
     gene            4337..>4796
                     /locus_tag="VGB76_02240"
     CDS             4337..>4796
                     /locus_tag="VGB76_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0097748.1"
                     /translation="MQHANAVAPVATQPLINYNALPGIPCPICASEKYEIVTLRFDGG
                     RIVQCLQCGHKYLNPTLTDDILKAIYTDYHEAESDESVIAMVSNWFAQPDSPYQYVLQ
                     KLELDGGLNGKRVVEVGCGPGKFLNECRLRGAIVAGVDISPGAVRMAKNFF"
BASE COUNT         1040 a         1300 c         1373 g         1083 t
ORIGIN      
        1 tgacgacgtg gcaggagtca tcggaactca tatctacggt atgccgtgtc acgtggatga
       61 gttgcagagg gtcgtcgctg attatgcgca gcggcagtca cgtcgcgtct tcctgctgta
      121 tgatgccgcg cacgctttcg gttcgtctat acgagggcgt cgcgtgggaa gttttggcga
      181 cgccgaggtg ttctcgctgt ccgtcaccaa gatgctggtc agtgtcgaag gtgggatgat
      241 ttccagccgg caccccgaac tgatcgggcg catccgtaaa atgcgcaatt acggcatcga
      301 ggccaactac gacacctact ggccgggcat gaacggcaag atgagcgagt ttcacgctat
      361 catcggactc cataaccttc gtcgcctcga tgaactgctg gccgagcgac agcacaaagc
      421 taaatattat ttcacacgca tacacgaacg caccggattt gaaactctgc cgtggccgga
      481 taatgtggtg cacactttca aggacttaac cgttctggtt cctgaacgaa gaatcggaga
      541 gcgtgatgag gtggtagcct tcctgaagac acagggaatt gaaacccgcg cctattttta
      601 cccgcctgtt cacgaacagc gattgtttca gagttttgcc gaccgcgcac tgccgcgcac
      661 ggaatcactt tcccgccgcg tcatcacact gcctttctac accgccatga cagaggacga
      721 gatggattgc gtagtacagg cattacgtct gtatgaggaa ggtaagtgaa ctgcgagtgc
      781 gaggcgttca gaaagctgcc tgagcgaatt cctctccgta aaaaccaccc cactataagt
      841 ctataaacat ggaatgaata tacttgtaac aggcggcgcg gggtatcttg gctccgtgct
      901 tataccaaaa ctactggcgc gcggtcatcg tgtgcgcgtg ctggatttag gctacttcgg
      961 tctgggacat ctgcgcgctc tgcgacccag cgttgaggtg atccgtgaag acctgcgcgc
     1021 cctggcagcc gctccagccc agatgcatgc gctcctcgat ggctgcgacg tcgtcattca
     1081 tctcgctgcc gtttcaaacg atccttcggc tgaactgaac ccggggctga cggaagaggt
     1141 gaacttccac gccaccatcg cgctcgccga ggcagcgcgt gcgctgggtg tgaagttcct
     1201 gttctcgtcg tcgtgctcgg tctacgggga ggcagagggc gagttagacg aggaaagttg
     1261 ggtcaacccg ctgacggcct acgcaagctc aaaagttaaa gcggagcagg ggctttcagc
     1321 cctcgcgcag accggctggc ggccagttat cctgcgtaac ggtacgctgt tcggttactc
     1381 gccccgcatg cgttttgatc tggtcgtcaa catctttagc ctctatagca cgttgcacaa
     1441 tgagatcaaa atctttggcg acggccagca gtggcgtcca ttccttcacg tcagcgactg
     1501 cgcgcgcgct tttgtcttct tcacagagca acccgaacaa cagcacctct gctataacat
     1561 cgcgcatgag aatctacgtg tggtcgacat cgcggcgcgc ttcatgcaat tgaatccatc
     1621 gttgcaggcg acacacgttg agactccgga tgaagaccgg cgcgactatc gcgtctcgac
     1681 cgaaaggatc ggagccgaag ggtttcgcac gcgaaccggc gtggaggtgg gagccgaaga
     1741 gatgatcgag gccatcatta acgggttgat tcctgacccg gaatcaatct tctatcgcaa
     1801 cgccaagtgg ttgaaggagt tgacgcagat cggctcgaag aaccatcgtg atatcgtgag
     1861 cctgttggaa acactctcgc acctcaatct gccaacccgc gtctgaagtt gtctctgtgc
     1921 tgtcagtgga aaacgtaagc taaggatgaa tccgaaccgc acggtgcagg taatcattgt
     1981 cggggctggt gggcacggaa gcgaacttca cgcctacctg cgcgaactct gcgtgcaagg
     2041 ctccggtatc gagtttctcg gcttcatcga cgatcacagg ccgcgcggcg cgtggggcga
     2101 cacagaggtt ttaggcgggc tggcctgttt agaagagttt ttaggggcgc gggtgggcga
     2161 actccactac atcacagccg tcggcagtaa cacgctgcga cgcactttcg ttgaacgatt
     2221 gcgccggatc gacgagcaac gtctaaagcc ctggacgctg caacatccac gcgcaattac
     2281 cggccacggc gtggaattcg gcgcgggcac gtgccttgca ccgggcagca ttgtgacgac
     2341 taacgcgcga atcggctcgc actgtattct caacgttaat acttctgtat cccacgacgc
     2401 cgtggtcggg gattatacaa acctcaatcc gggggtcgtc gtagccggaa acgtagagat
     2461 cggagagggg tgctacatcg gcgcgggtgc gactttgatt gacaacgtga agattggaga
     2521 atggtcaact atcggcgcgg gcgcggtcgt tattggcgac atccctcccc gcgtgacggc
     2581 ggttggcgtt cccgcacggg tcattaaata tcatgagtaa tatgatgccg ctcatcacat
     2641 ttattatccc ctgctataac tatgcgcgct atttgccgga ctgcctcaat agtattttac
     2701 agcaagaggg caagtttgat ttcgagatca ttctaattga tgacgcttcg accgacgata
     2761 cgggcgaggt catacgttcc tacaacgacc cacgcattcg ggtgattaca catgccgtaa
     2821 acgtagggca tgccgggacg atcagcgaag ggttgaaatt agcccgtgga gagtatgtcg
     2881 cacgcattga tccggacgat cgctaccgcc cgggttactt atcacgcgtg ataccgaagt
     2941 tcgaggcgca cccggaagtc gggctggttt acgggaatgt gtcgttgatc gatgagcatg
     3001 gcgcaacttc cgtggggcgc ggcgaccggg tgcacggcgg gcgcgactac aaggggtgcg
     3061 agctggtggc gctgttgggt caaaacttta tctgcgcgcc ctcggttatg gcgcgtcgag
     3121 aagcatggct taaggcactg cctgtgcccg agtggttagc atttaatgat tggtatttta
     3181 cgctgatgat ggctaggcag tacgagtttt attacctcga cgaggtgctg gccgactacc
     3241 gcgtgcatgg cacgaaccac cacagccgta tcgtgctgaa caaaacagaa gagccttcga
     3301 tattttggct gctggataag atttttagta cgccggaaac gagccccgaa cttgagattg
     3361 ccaaacagcg cgcccgtcgc cgaatctacg gagcgcagta tctcgacatg gctgataagt
     3421 acttcggctg cgattactgt ggcgacgccc gccgctgtta tctcgccgca ctgcgtcatc
     3481 atccggcgta tcttctgaat ccgggcgtcc tccgccgtct gcttgctact ttcattggac
     3541 gcaaaactta cgaatcctcg aagtcaaccg tcaaagctgt tctcgcaccg ctcgcaagag
     3601 tgcgccgtta gcgttatgaa aataacgaca cgcattgcct attctctctt cccctcatat
     3661 gtctggggca ctcttcgcga ggaattaaaa ctcactagat gtcgctggaa ggcacgccgc
     3721 cgggttgacg gacacatatt tgcgggcagc ggttataagc tacatctcgg cagtggaccg
     3781 cgtgttgtgc ccggttggtt taatattgac ggcgctcccc aagaccaact cgacctgcaa
     3841 tgggatctgc gcacgccgct tcctttcgcc aatcgaagtt gccgcttaat ttattcggag
     3901 catttgctgg aacacctctt caaagaagac gcactcaatt tgttgcagga atgcaaccgt
     3961 ctgcttgagc cgggaggcag catccggctc ggcgtgccgg acgccgaatt gtacctgcgc
     4021 gcttacgtcg aacgccggac agactttttc cagggtctta agcatttggg tggcgccgtc
     4081 attccgctct ccactcctat tgacgtaatc aatcaaatgt ttaggatggg cggagcgcac
     4141 ctatttgcat gggacttcga aacaatcgaa cgcgcactcg ttgatgcggg cttcacacag
     4201 atcacacgtt ggccttccgg tcaggcttca tctccggaac tatgcctgga tgaccccgag
     4261 cacgcttttg aaactttgta cgtagaggct gtgaagccgg gcgtttaaga cgcaagctat
     4321 cgctccgcca gagactatgc agcacgctaa cgccgtcgca cccgtggcga cgcaaccctt
     4381 gattaactac aacgcgctcc cgggcattcc ttgtccgatc tgtgccagcg agaaatatga
     4441 gatcgttact ttgcgttttg atggcgggcg catcgtgcag tgcttacagt gcgggcacaa
     4501 atatctgaat ccgacgctga cggacgatat tctcaaggcc atctatacgg actatcacga
     4561 ggcggagagc gatgaatccg tcatcgcgat ggtttcaaat tggttcgcgc agccggatag
     4621 cccatatcaa tacgtgctgc aaaagctcga actggatggc gggttgaacg gtaagcgtgt
     4681 cgtcgaagtg ggttgtggtc cgggcaagtt tctaaacgag tgccgcttac ggggtgctat
     4741 cgtggcaggc gtcgatatca gtccgggcgc ggtacggatg gcgaaaaact tctttg
//
LOCUS       DASWNS010000062         6859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_443757, whole genome shotgun sequence.
ACCESSION   DASWNS010000062 DASWNS010000000
VERSION     DASWNS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443757"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VGB76_02245"
     CDS             <1..800
                     /locus_tag="VGB76_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0097749.1"
                     /translation="SSGNAVLICAGQTRVLVDAGMSAKEIVRRLALVGCDAATLDGVI
                     ITHEHGDHAGGLRVLLKTLSCPVYISGETRDAYIGDREGVTRDEPRKRDDALRDRTEE
                     ISSSRDFRIGEIDFHPFTIPHDAADNFGFTARHEGVCVATLMDFGHITTLINERLRGC
                     AAVIIESNHSRDMLKACSVYPWELKQRILSRTGHLSNEDVAVWLRDEYDGGAEHIVLA
                     HLSQRANNPYVAKLSAEVALQERGSLFSTDTKITLSFPKEPTPWIEL"
     gene            785..1264
                     /locus_tag="VGB76_02250"
     CDS             785..1264
                     /locus_tag="VGB76_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HEY0097750.1"
                     /translation="MDRTMSAPPLMPRALRLSTAATLLLLVALSFACGVEQKRSGLPP
                     DAQAAIDSITSDMGRGDYEKIYAEAAEEWRRTASTGESRANLERIRSALGNVLSRAQL
                     SAQERENASGDLTGHTLVVSYNTKFERADAIETYTLVERSGRWLLARYAVNSNVLRQ"
     gene            1403..2719
                     /gene="purB"
                     /locus_tag="VGB76_02255"
     CDS             1403..2719
                     /gene="purB"
                     /locus_tag="VGB76_02255"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY0097751.1"
                     /translation="MINRYTLPEMGALWSEAAKFQKWLDVELAVCEVHAEMGTIPEDA
                     LAEIKARAAFTVERINEIERTTDHDVIAFTTALAEKIGESARFVHYGLTSSDVVDTAN
                     ALLLRDACDLLLKRIDALLEVLKRRAYEFKETPQIGRTHGVHAEPTSFGLTFALWYDE
                     MRRNRERLELARARVAVGKISGAVGAFAHLDPEVEERVCARLGLRAAPVSTQVIQRDN
                     YAEYLSTLAIVASSLDKFALQIRHWQRTEVREAEERFKKGQKGSSAMPHKRNPILSER
                     ICGMARVVRGYAIAGLESVALWHERDISHSSAERVILPDSSIALDYMLAKATSLIDTL
                     VVYPARMLENLNATRGLVFSGQLLLALTRGGVSREQAYEWTQRNAMKTWDEGGDFRTR
                     VLEDSDIGAHLSRAEIERAFALDTYLRNVGAVFTRVFGERAATNDG"
     gene            2782..3585
                     /locus_tag="VGB76_02260"
     CDS             2782..3585
                     /locus_tag="VGB76_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097752.1"
                     /translation="MFKFVSLMLAVLLSAAAVAGQRNAPAAPAELAEITERGRQLVEY
                     DQAAWHATDAVVALSPPEGSVARYIARKTAAGWVVGFGRFNEKRDRFLVVYEATQGAS
                     PTEFKVRKHEPPQEAADFYLFAAKAIEIALADFKGENRPYNVAVLPAKPEGLYVYLVP
                     AQTKHGIYPHGGDVRYLISADGSKLVSKRQMHKAIIEIDAPANTKEVAAGFHTAVMDD
                     IPEDTDVFYVLARKPAVPEWIGTRKYVYKVEVDGTIKYVMTTEAFRKMK"
     gene            3610..3855
                     /gene="purS"
                     /locus_tag="VGB76_02265"
     CDS             3610..3855
                     /gene="purS"
                     /locus_tag="VGB76_02265"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY0097753.1"
                     /translation="MVKAKVYVTLKPGVLDPQGKAIRNSVGLLGYGGIADVRQGKYFE
                     IALDESLEGERARETAERMAREVLANPVIEDYRVEIE"
     gene            3855..4553
                     /gene="purQ"
                     /locus_tag="VGB76_02270"
     CDS             3855..4553
                     /gene="purQ"
                     /locus_tag="VGB76_02270"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY0097754.1"
                     /translation="MKFGVIVFPGSNCDHDAYHVISKHVGQPVSFIWHRETDLTDYDA
                     LIIPGGFSYGDYLRAGALASLSPVMSSVQEFAARGGLVLGICNGFQILCEAGLLPGAL
                     IHNRDLHFVCQHVNVRVENADTPFTSELKRGQILSMPVAHAEGNYVCDDETLAMLRRE
                     DRIIFRYCDAQGNLTEAANPNGARDHIAGICNRERNVVGLMPHPERACEDLLGSSDGR
                     GLFHSLAATLASVS"
     gene            4743..6281
                     /locus_tag="VGB76_02275"
     CDS             4743..6281
                     /locus_tag="VGB76_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0097755.1"
                     /translation="MRTSKLALCLGLFIALTHGFGESARAQTKELTASEQSYREARRV
                     LDAGLAAYGGLDALRTIESTTIRHEGFTVHRNQSRRTDPPYERTPLSGTLILEPRRGR
                     LYHDNKGSYPGGFNWHWGLMTNGKETARVTFLERRINSTAALPPQAMRGRLRWLPHNI
                     LINALERAVQLRSMGRANFDNRPHDVVTYANEDGVQLTVYLDAGTHLVSKFELLGSDP
                     YFGDSVQEVIFPAHRAVEKFQVPTARVTKIMGEVVEEIPYVEVAFNKSHGDEQFKAPA
                     GFSAPVAAPPVETPPVNRLAENVYMIRGGGYNVLAVGFKDHVMVVETPGGDSTSREII
                     TRIKELMPGKPIRYAAVTHHHDDHAGGVRTYIAEGATIITTPALRSFFTRVAAARNFT
                     FQPDALTRNPRPLVLETLQNGKRIFTDETQTVELYDIGSGPHMDEMIVAYLPKEKIIF
                     QGDLLNRPEDGHIAPGNATTAHFAGWLKKSGLAVERIVGVHGPVATPDDLQKAVALIQ
                     QSQN"
     gene            6383..6796
                     /locus_tag="VGB76_02280"
     CDS             6383..6796
                     /locus_tag="VGB76_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097756.1"
                     /translation="MNYPATPPDEILSWAEVQRTHRIRNGVYQRGGRLISLLTDFGRI
                     NPCYPDAHADTSGETIIYTGAGRRGDQHLTPPNRALLSAIESGHAVPLFNKLSVGRWQ
                     HMGYWRVVHATHQHDAEEDRMLWRFTLKKALTSDE"
BASE COUNT         1386 a         2252 c         2031 g         1190 t
ORIGIN      
        1 gctcatcggg aaacgccgtg ctgatctgcg cggggcagac gcgcgtgctc gtggatgcgg
       61 ggatgagcgc gaaggagatc gtgcggcggc tcgcgctcgt cggctgcgac gccgccacgc
      121 tcgacggcgt gatcatcacg cacgagcacg gcgaccacgc gggcggcctg cgcgtgctct
      181 tgaaaactct ctcctgcccc gtctacatct ccggcgagac gcgcgacgcc tacatcggcg
      241 accgcgaagg cgtgacccgt gacgaaccgc gcaaacgcga cgacgccctg cgcgaccgca
      301 ccgaagagat ctcttcgagc cgtgatttcc gtatcggcga aatcgacttt cacccgttca
      361 ccatcccgca cgatgcggcg gacaacttcg gcttcaccgc gcgtcacgaa ggcgtctgcg
      421 tcgccacgct catggacttc ggccacatca cgactttgat caacgagcgt ctgcgcggtt
      481 gcgctgccgt catcatcgag tcgaatcaca gccgcgacat gctcaaggcg tgcagcgtct
      541 acccctggga gttgaagcaa cgcatacttt cgcgcacggg gcatctctcg aacgaagatg
      601 tcgccgtctg gctgcgcgac gagtacgacg gcggagccga acatatcgtg ctcgcgcatc
      661 tctcgcagcg cgccaacaat ccctacgtcg ccaaactctc ggcggaggtc gcgttgcagg
      721 aacgcggctc gctcttttcg accgacacca agatcacgct ctcgtttccc aaagagccga
      781 ccccgtggat cgaactatga gcgcgccgcc cctcatgcct cgcgcgctgc ggctctctac
      841 ggcggcgacg ctcctccttc tcgttgcgct cagcttcgcg tgcggcgtcg aacagaaacg
      901 aagcgggttg ccgccggacg cgcaggcggc gattgatagc atcacgagcg acatggggcg
      961 cggcgactac gaaaagattt acgcggaagc ggcggaagaa tggcggcgca cggcgagcac
     1021 gggcgagagc cgcgccaatc tcgaacgcat ccgcagcgcg ctcggcaacg tgctcagccg
     1081 cgcccaactc agcgcgcagg aacgcgaaaa cgcgagcggc gacctgaccg gccacacact
     1141 cgtcgtcagc tacaacacaa agtttgagcg cgccgacgcc atcgaaactt acacgctcgt
     1201 cgaacgctca ggccgctggc tgctcgcgcg ctacgcggtg aactcgaatg ttctaagaca
     1261 gtgacaggtg acgggtgaca ggtgacaaga taagagaaga gtgacgagtg acgggtgaca
     1321 agttaaaatc ctgtcgcctc ctgctcatcg cttctccctc gtcgcttctc cctcgtcact
     1381 tgtcactcgt cactgctttt ttatgattaa tcgttacaca ctgcccgaga tgggcgcgct
     1441 ctggtcggag gcggcgaagt ttcagaagtg gctcgacgtg gagttggccg tctgtgaagt
     1501 gcacgcggag atgggcacga tcccggagga cgcgctcgcc gagatcaagg cgcgcgccgc
     1561 cttcaccgtc gagcgcatca acgagatcga gcggacgacc gaccatgacg tgatcgcctt
     1621 caccaccgcg ctcgccgaga agatcggcga gagtgcgcgc ttcgtgcatt acggcctcac
     1681 ctcttccgac gtggttgaca cggcgaacgc gctgctcctg cgcgacgcct gcgatctgct
     1741 cttgaaaagg atcgacgcgc tgctcgaagt cctgaaacga cgcgcctacg aatttaaaga
     1801 gacgccgcag atcgggcgca cgcacggcgt ccacgccgaa ccgacttcgt tcggcctcac
     1861 ctttgcgctc tggtacgacg agatgcgccg caaccgcgaa cggctcgaac tggcgcgcgc
     1921 gcgcgtcgcc gtcggcaaga tcagcggcgc ggtcggcgcg tttgcgcatc tcgacccgga
     1981 agtggaagag cgcgtgtgcg cgcggcttgg gctgcgcgct gcgcccgtct ccacgcaggt
     2041 cattcagcgc gacaactacg ccgagtatct ctcgacgctc gccatcgtcg cctcttcgct
     2101 cgacaagttc gctttgcaga ttcgccactg gcagcgcacc gaagtgcgcg aggcggagga
     2161 gcgtttcaag aaaggccaga agggatcgtc ggcgatgccg cacaagcgca acccgatcct
     2221 ctcggagcgc atctgcggca tggcgcgcgt ggtgcgcggc tacgccatcg ccggactcga
     2281 atccgtcgcc ctctggcacg agcgcgacat ctcccactcg tcagccgaac gcgtcatcct
     2341 ccccgattct tcaatcgcgc tcgactacat gctcgccaaa gccacgtcgc tcatagacac
     2401 gctcgtcgtc taccctgcgc ggatgctcga aaatttgaat gccacacgcg ggcttgtctt
     2461 tagcggtcaa ctgctgctcg cactgacgcg cggcggcgtc tctcgcgaac aggcttatga
     2521 atggacgcag cgcaacgcca tgaagacctg ggacgaaggc ggcgacttcc gcacgcgcgt
     2581 cctcgaagac tcagacatcg gcgcgcatct ctcgcgcgcg gagatcgaac gcgccttcgc
     2641 gctcgacacc tacctgcgca acgtcggcgc ggtcttcacg cgcgtgttcg gcgagcgtgc
     2701 ggcgacgaac gacggttgat gcctgaaccg gagcgtgacg atacgcgtta cgggcgcggg
     2761 catgtttttc ggggggatga gatgttcaag tttgtaagtt taatgctggc ggttctgctt
     2821 tcagccgcag ccgtcgcggg gcagcgcaac gcgcccgccg ctcctgccga gttggcggag
     2881 atcacggagc gcgggcggca actcgtcgag tacgatcagg ccgcctggca cgcgacggac
     2941 gccgtcgtcg ctttgtcgcc gccggaaggc agcgtggcgc gctacatcgc acgcaagacc
     3001 gccgcggggt gggtcgtcgg cttcggacgc ttcaacgaaa agcgcgacag gtttctcgtc
     3061 gtctacgagg cgacgcaggg cgcgagtccc accgagttta aggtacgcaa gcacgagccg
     3121 ccgcaggagg ctgccgactt ttatctcttc gcggcgaaag ccatcgagat cgcgctcgcg
     3181 gatttcaagg gcgagaaccg gccttacaac gtcgccgtct tgcccgcgaa gcccgaaggg
     3241 ttatacgtct atctcgtgcc cgcgcagacg aagcacggca tctatccgca cggcggcgac
     3301 gttcgctatt tgatctccgc agacggctcg aagctcgtct cgaagcgtca aatgcacaaa
     3361 gcgatcatcg agatcgacgc gcccgctaac accaaagagg tggccgccgg atttcacacc
     3421 gcagtgatgg acgacatccc cgaagacacg gacgtgttct acgtcctcgc acgcaagccc
     3481 gccgtgccgg agtggatcgg gacgcgaaaa tatgtttata aggtagaggt tgacggcacg
     3541 atcaaatatg ttatgacgac ggaagcgttt aggaagatga aataagtttg cggcgagagg
     3601 acaagcaaga tggtaaaagc taaagtttac gtgacattga aaccgggcgt gctcgatccg
     3661 cagggcaagg cgatccggaa ctcggtgggg ctgctcggct acggcggcat cgcggacgtg
     3721 cggcagggca agtatttcga gatcgcgctc gatgaatcgc tcgaaggaga gcgtgcgcgc
     3781 gaaaccgccg aacgcatggc gcgcgaggtg ctggcaaatc ctgtgatcga agattatcgc
     3841 gtggagatag agtaatgaaa ttcggcgtta tagtttttcc cggctcaaac tgcgaccacg
     3901 acgcgtatca cgtcatctcg aagcatgtcg gccagcccgt ctccttcatc tggcaccgcg
     3961 aaaccgacct gacggactac gacgcgctca tcattcccgg cggcttctcc tacggcgact
     4021 acctgcgcgc gggcgcgctc gccagcctgt cgcccgtcat gtcgagcgtg caggaatttg
     4081 cggcgcgcgg cggactcgtc ctcggcatct gcaacggctt ccaaattctg tgcgaagcgg
     4141 ggctgctccc cggcgcgctc atccacaacc gcgatctgca tttcgtctgc cagcacgtca
     4201 acgtgcgcgt ggagaacgcc gacacgcctt tcacgagcga actcaagcgc ggccagattc
     4261 tctccatgcc cgtcgcgcac gccgaaggca actacgtctg cgacgacgag acgctcgcca
     4321 tgctccggcg cgaagaccgc atcatctttc gctactgcga cgcgcagggc aacctcaccg
     4381 aagcagccaa ccccaacggc gcacgcgatc atatcgcggg catctgcaac cgcgagcgca
     4441 acgtggtggg gctgatgccg cacccggagc gcgcctgcga agacctgctc ggctcctcgg
     4501 acgggcgcgg cctcttccac tcgctcgccg ccacgctcgc ctccgtctcc taaaagaaga
     4561 aacgacgaag gcggaacgat aaggacgaaa tgaaagcaag aaccttttgt ctttcattcc
     4621 gccttcatca ttccgccttc gtcgtctgtt ttctacggaa cttccttgcg cccccgtcag
     4681 tatttagtag acattggggc aagtctcccg ccagacaaca tccagtaagg agaatctcag
     4741 ctatgcgtac ctcgaaactc gctctctgtc tcggtctgtt tatcgcgctc acacatgggt
     4801 ttggcgaaag cgcgcgggcg caaacgaagg aattgacggc ttccgaacag tcctatcgag
     4861 aggcgcgtcg cgtgctcgac gccggactcg cggcctacgg cggactcgac gccctgcgca
     4921 cgattgaaag cacgacgatc cgccacgaag gcttcaccgt gcatcgcaac cagagccgcc
     4981 gcaccgaccc gccctacgag cgcacgcctc tgagcggcac gctcatcctc gaaccgaggc
     5041 gcgggcgtct ctatcacgac aacaagggca gctatcccgg cggcttcaac tggcactggg
     5101 gcttgatgac gaacggcaaa gagacggcgc gcgtgacctt tctcgaacgc cgcatcaact
     5161 ccaccgccgc gctcccgccg caagccatgc gcggtcgcct gcgctggctg cctcacaaca
     5221 tactcatcaa cgcgctcgaa cgcgccgtgc aactgcgttc gatgggacgc gcaaacttcg
     5281 ataaccgccc gcatgatgtc gtgacctacg ccaacgagga cggcgtgcag ttgaccgtct
     5341 atctcgacgc cggaacgcat ctcgtctcga agtttgaatt gctcggcagc gatccttatt
     5401 tcggcgactc ggtgcaggag gtcattttcc cggcgcatcg cgcggtcgag aagtttcagg
     5461 tgccgacggc gcgcgtcacg aagatcatgg gcgaagtggt ggaagagata ccttacgtcg
     5521 aagtggcctt caataagagt catggcgacg agcagtttaa agcgcccgca ggattcagcg
     5581 cgcccgtcgc cgcgcctccc gtcgaaactc cgcccgtcaa tcgtcttgcg gagaacgtct
     5641 acatgattcg cggcggcggc tacaacgtgc tcgccgtcgg cttcaaagac cacgtgatgg
     5701 tcgtcgagac tccgggcggc gacagcacct cgcgcgagat catcacgagg atcaaggaac
     5761 tgatgccggg caagccgatc aggtacgcgg cggtcacgca tcatcacgac gaccacgcgg
     5821 gcggcgtacg cacttacatc gccgagggcg cgacaatcat cacgacgccc gccctgcgct
     5881 cctttttcac gcgcgtcgcg gcggcgcgaa acttcacctt ccagcccgac gcgctcacgc
     5941 gcaacccgcg cccgctcgtc ctcgaaacct tgcagaacgg caaacgcatc ttcacagacg
     6001 agacgcagac cgtagagttg tatgacatcg gctccggccc ccacatggat gagatgatcg
     6061 tggcctacct gccgaaggag aagatcatct ttcagggcga cctcttaaac cgccccgaag
     6121 acgggcacat cgcgccgggc aacgccacga ccgcgcactt cgccgggtgg ctcaaaaaat
     6181 ccggcctcgc cgtcgaacgc atcgtcggcg tgcatggccc cgtcgccacg cccgacgacc
     6241 tgcaaaaagc ggtcgccctg atacagcagt cacagaactg aagtcctcga cgatgaacgc
     6301 ggaatgatga acgagaaaag ctgtccagcg ggttaacgcc tgcctgcttc attcatcctt
     6361 ccgcgttcat catttctctc caatgaacta tccggcgacg ccgcccgacg aaattctctc
     6421 ttgggcggaa gtccagcgca cgcatcgcat ccggaacggc gtctaccagc gcggcgggcg
     6481 gctcatttcg ctcctgaccg acttcgggcg catcaatccc tgctacccgg acgcgcacgc
     6541 agacacgagc ggcgagacga tcatctacac gggcgcgggg cgtcgcggcg atcaacatct
     6601 cacgccgccg aatcgcgccc tgctctccgc catcgagagc ggccacgccg tcccgctctt
     6661 caacaaactc tctgtcggac gctggcagca catgggctac tggcgcgtcg tccacgccac
     6721 gcaccaacac gacgcagaag aagaccgcat gctctggcgg ttcaccctca agaaagcact
     6781 tacaagtgat gagtgatgag taatgagtga tgagtaaaaa cagaagattt gtctttaact
     6841 catcactcat tactcatca
//
LOCUS       DASWNS010000063         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_470357, whole genome shotgun sequence.
ACCESSION   DASWNS010000063 DASWNS010000000
VERSION     DASWNS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470357"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VGB76_02285"
     CDS             <1..197
                     /locus_tag="VGB76_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097757.1"
                     /translation="AEMNEIFERIAIELRHQYSIGYKPKNFVNNGKWHKLKVKVTPPR
                     GLPRLFVRTKEGYFATTQPR"
     gene            488..1732
                     /locus_tag="VGB76_02290"
     CDS             488..1732
                     /locus_tag="VGB76_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0097758.1"
                     /translation="MPHRKVNHTGRRGRRNNGGKSVRLFLSLAFVAAALAFLSVVNVA
                     THAQRASDDSRPRRVANSQPQQQQTRPTPLPSPVPQLQTPAAQQTPTPPVLQDPGQDV
                     DEDDVVKVDTSLVNLNVRVIDRANRPVDDVRQEEFKIFEDGVPQQIFSFTRAEVPISY
                     GIVVDNSGSMKTQLEKVIDATRTIIQQNKPGDETFLERFVSSDEIAILQDFTANQEDL
                     VDALDKMHTEGGQTAVLDAVYLAAEHVGKHKKGDPLSDKRRRALILVTDGEDRSSFYK
                     QAELFEFLRENDVQIYVIGFVNELDSQGSFIRKSSKDKAVNLLNKLATETGGRAFYPM
                     SLAELPGIANEITKDLRTQYVVSYSPTNKRRDGTFRKVNIRIEDTGKRDKRIAVTRPG
                     YTAPRGEAAAPARTSSTSNKQP"
BASE COUNT          415 a          603 c          523 g          285 t
ORIGIN      
        1 ccgccgagat gaacgagatt ttcgagcgca tcgccatcga actgcgccac caatattcca
       61 tcggctacaa gccgaagaat ttcgtcaaca acggcaaatg gcacaagttg aaggtgaagg
      121 tgacgccgcc gcgcgggctg ccgcgcctct tcgtgcgcac gaaggaaggc tacttcgcca
      181 ccacacagcc gcgctagagc ctgcgccgcg cccgtacgag ccgcgcgatt ctcccggctc
      241 tcactggcaa cgcgccgcgc gcccggctcc tctaaaggtg caggcggcgt gactcgcacg
      301 acgcgcatca gttcttttta gccccagccg cgcgaagcca atttttggct gacaaacgaa
      361 gcgccccgcg cgccgtcccc gcctttttcg aatggcggac gggggcgtcg ccgggcgctt
      421 ccttgtgctc gtctcgaagc gtcggagcgt ggctatcgct ggtttttaag tcgcaggaga
      481 agacgggatg ccacatcgca aagttaatca cacaggccgt cgcggtcgtc gcaataacgg
      541 cggcaaaagc gtgcgcctct ttttgtcgct ggccttcgtc gccgccgcgc tcgccttcct
      601 gagcgtcgtc aacgtggcga cgcacgcgca gagagcctcg gacgattcac gcccgcgccg
      661 cgtggcgaac tcgcagccgc agcaacagca gacgcgcccg acgcccctgc cgagtcccgt
      721 cccgcagcta cagacgcccg ccgcgcagca gacgcccacg cctcccgtgc tgcaagaccc
      781 cggacaggat gtggacgaag acgatgtcgt caaagttgac acgagcctcg tgaacctgaa
      841 cgtgcgcgtc atcgaccgcg cgaaccgtcc cgtggacgat gtgcggcagg aggagttcaa
      901 aatcttcgag gacggcgtgc cgcaacagat attctctttc acgcgcgccg aagtgccgat
      961 cagctacggc atcgtcgtgg acaactccgg ctcgatgaag acgcagttgg aaaaagtgat
     1021 cgacgcgacg cgcaccatca tccagcagaa caaacccggc gacgagacgt ttctcgaacg
     1081 cttcgtcagc agcgacgaga tcgctatctt gcaggacttc accgcgaatc aggaagacct
     1141 cgtggatgcg ctggacaaga tgcacaccga aggcgggcag accgccgtgc ttgacgccgt
     1201 gtatctcgcc gccgaacacg tcgggaaaca caagaagggc gaccccttaa gcgacaagcg
     1261 gcgacgcgct ctcatcctcg tcaccgacgg cgaagaccgc agcagtttct acaaacaggc
     1321 ggaactcttc gagtttctgc gcgaaaacga cgtgcagatt tacgttatcg gcttcgtcaa
     1381 cgaactcgat tcgcagggca gtttcatacg caagagttcg aaggacaagg ccgtgaacct
     1441 gctcaacaaa ctcgccaccg agacgggcgg gcgagccttc tatcccatgt cgctcgcgga
     1501 actccccggc atcgccaacg agatcacgaa agacctgcgc acgcagtacg tcgtcagcta
     1561 cagcccgacc aacaagcggc gcgacggcac gtttcgcaaa gtaaacatcc gcatcgaaga
     1621 tacaggcaaa cgcgacaagc gcatcgccgt cacgcgcccc ggctacacag ccccgcgcgg
     1681 agaggccgcc gcgcccgcgc gcacatcttc aacttccaac aagcaaccgt aactacaggg
     1741 acaagtgatg agtgacgggt gacgagcaag accgaagaag taacaaagtg aagcgtcact
     1801 cccgaataga cgaaacgctg ctttcg
//
LOCUS       DASWNS010000064         5479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_365804, whole genome shotgun sequence.
ACCESSION   DASWNS010000064 DASWNS010000000
VERSION     DASWNS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365804"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..890)
                     /locus_tag="VGB76_02295"
     CDS             complement(114..890)
                     /locus_tag="VGB76_02295"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0097759.1"
                     /translation="MSYKPLELNGRVAVVIGGTSGIGRAIAHGLAEAGADVVPTSRRM
                     EQVEAAAAEIEERGRRSLRVVSDVSERDSLQRVLAASVEAFGKVDILVNSAGRTKRAP
                     TVDFPEDDWNDIIETNLTGTLRACQVFGRHMLEREYGRIINIASLSTFVALYEVAAYS
                     ASKAAVASLTKSLAVEWASRGVNVNAIAPGVFRTALNQKLLDETERGREFQLRTPARR
                     FGKVEELAGAAVFLASEAASFVNGEVLVVDGGFLASGVNQ"
     gene            1285..2217
                     /locus_tag="VGB76_02300"
     CDS             1285..2217
                     /locus_tag="VGB76_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097760.1"
                     /translation="MSAPNTETFTRRVAGNNVWLAYGGGADPVAALQPRELRYEILES
                     EEAVGRAQYEELQRAAREKDGDLVIILLGGRGAQAMYRILGELAEGTQADELLARLHV
                     FTQDALAPMRMDNGLSFVRDFERLLGRAFFAKVKSFTPMQTDTEDLEGGLAGYLEKLA
                     SLGGIDIFFLGLGPEAGEASHLCYIKPGSGATARDVAGVIPISPSILEHHITKFKVGG
                     SAVTEADEAECRAATHILTLGPAAILGARRIVQSIVDASTAPAKRASYRRVLETEIEE
                     DAARRAAQCDENPGLWLRLHPNVRSLVLPDVFQA"
     gene            2252..3823
                     /locus_tag="VGB76_02305"
     CDS             2252..3823
                     /locus_tag="VGB76_02305"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY0097761.1"
                     /translation="MSSMITQAQPVSEYAIIVSPEDNLAVVKRETAPGLELALPGGRV
                     IRVAATVPPGHRFATRDIPQGEFVLQYGQPIGTSLGITEGDYISHANMSDDVPIVRDL
                     PEDLQTPPPDYLPVSERATFKGFRRPDGRVGTRNFVLIVPTSMCASHEAQQISMMSEF
                     LHYKRERYPNVDGVVAIPHNKGCGCQDGSTIDVMLRTLSNYADHPNVGGVILMDLGCE
                     KTNLAKVEQYLLKRDKNFDKPVAKIGIQDVGGTQAAIEQGLREVQRMLPEVNRIEREE
                     VSVSELVLGVKCGGSDGFSGISANPSLGRAADHLVRSGGTVLITEVPEFCGAEHILAN
                     RAKDAATGRAIYRMIDWYKEYASKFGAVLNQNPSPGNIAGGLLNITIKSLGAMAKAGT
                     TRVEGTVDYAETPQGRGLYLMQGPGYDQESTPGLVAAGANVVVFTTGRGTTIGNAIVP
                     VIKLASNNVVFERMSRDLDLSAGNVIEGTETIDEVGARVFEHVRRVAGGEVLAKAEEH
                     KHREFQFWAEQTVSL"
     gene            4010..>5479
                     /locus_tag="VGB76_02310"
     CDS             4010..>5479
                     /locus_tag="VGB76_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0097762.1"
                     /translation="MPTLRDVATLAGVSVTTVSNVVNDARRVLPATRARVEEAMAQLN
                     YAPARGVAGTSASPLPADGHTRPTASSFAPAIQDRSRTGDTTGFRAYAVAAAADPGTP
                     GKAARMLLRLLRAAQPISRVELARRLGVNRSTVTDIFKPLIASGLVREELGAGTDRVL
                     GRPPVALSFNDDHVFFAGVNIGIRRVQVGLGTLGGEIVYEDDFDTPHDPAEALSLVRE
                     RIARLCAGGVGRTLRMIGVSVSGMTDGGRSRLVYAPHLGWHDVRIADALRFNPAGGVA
                     APDEEAVPVVVENDATAAAIYESRLGLRDRDVNLMDNFVLVRSGTGIGVGLVLGGEVY
                     RGAGRGEGLAGEFGHMTIVAGGKACVCGNRGCWERYASASSAASLYAGDRLQTNGAAR
                     PRYVEIVARAEAGELRARRTLEHVGEYLGIGIGNVIMGLGVPRVILSGRVVLGWKFIH
                     EPLRNAVAQSMAGRAGGWSVEPGEPRGAGLGGALEVAAEG"
BASE COUNT         1060 a         1721 c         1732 g          966 t
ORIGIN      
        1 caatcagcag ttagcgatca acattcggca atcagtaatc agcagtcagt gaagagtgtt
       61 gaaagtggcg ctgttttgaa ctcgtcactt gtcacccgtc acttgtcact gtcttattga
      121 ttgactccgc tggcgaggaa gccgccgtcc acgacgagca cttcgccgtt gacgaagctg
      181 gccgcttccg aagcgagaaa gactgccgca ccggccaact cctcgacctt gccgaaacgc
      241 cgcgccggag tgcgcaactg gaattcacgc ccgcgctccg tttcgtccaa aagcttctgg
      301 ttgagcgcgg tacggaagac gccgggcgcg atggcgttga cgttaacgcc gcgcgacgcc
      361 cattccacgg ccagagactt cgtgagcgag gcgaccgccg ccttgctcgc cgagtaggcc
      421 gccacttcgt agagtgcgac gaaggttgag agcgaggcga tgttgatgat gcgtccatac
      481 tcgcgctcaa gcatgtgacg gccaaagacc tgacaggcgc gcagcgtccc cgtcaggttc
      541 gtctcgatga tgtcgttcca gtcgtcctca gggaagtcca cggtcggggc gcgcttggtg
      601 cgtccggcag agttgacgag gatgtccacc ttcccgaagg cttcgacgct cgcggccaga
      661 accctttgca gggagtcgcg ttcggagaca tccgagacga cgcgcaggct cctccgcccg
      721 cgttcctcta tctcggctgc cgccgcctct acctgctcca tccgccgcga agtcgggaca
      781 acgtctgcgc cagcctctgc cagcccgtgc gcgatggctc gcccgatgcc agaggtgccg
      841 ccgatgacaa cggccacgcg gccatttaac tccagaggtt tgtaactcat tgtccggcta
      901 tcctgccttt aacggggctt acgctggtgg agacacttaa ggagaaccga gcgccccgaa
      961 tttttaatgt ttgaagaacc gcagccactt tagcaggcat atctgctgtc gggacatacg
     1021 ctttagaagg ttagaccaat taatgaacgc gcggaaggta tgtaacacca gcaggcataa
     1081 tctgtcaagc atcaaatctc tagtatattg acgttattgt taatttattg caaattacct
     1141 tgctttatca tgctgaatat ggtaaaaagc caccttcaca aacggggcaa tggttagacc
     1201 attgattaaa tcagcatcta caatctgcgg tgtggctggc actgttcagg cacagaagtg
     1261 ggaaaatcag cggaggttta agaggtgtcg gctcctaaca cagaaacatt cacgcggcgc
     1321 gtcgcgggca ataacgtctg gctcgcttac ggcggcggtg ccgacccggt cgcggcgttg
     1381 cagccgcgag aactccgtta cgagattttg gagtcggagg aagcggtcgg gcgtgcacag
     1441 tacgaagagt tgcagcgtgc ggcgcgcgag aaggatggcg atctggtcat catcctgctc
     1501 ggcgggcgcg gcgcgcaggc catgtaccgg attttgggcg agttggccga aggcacgcag
     1561 gccgacgaac tgctcgcgcg cctccacgtc ttcacgcagg acgcgctcgc gccgatgcgt
     1621 atggacaacg gcctgagctt cgtgcgcgac ttcgagcgtc tgctcggtcg agccttcttc
     1681 gccaaggtca agagtttcac gccgatgcag acggacacgg aagaccttga aggcgggctg
     1741 gcgggttatc tggagaaact tgcgtctctg ggcggcatag acatcttctt cctcggcctc
     1801 ggcccggagg cgggtgaggc gtcgcacctc tgctacatca aaccgggttc gggcgcgacg
     1861 gcgcgcgacg tggccggggt gatcccgatc tcgccgagca tactcgaaca ccacatcacg
     1921 aagtttaaag tggggggcag cgcagtcacg gaggccgacg aggcggaatg ccgcgcggcg
     1981 acgcacatcc tgacgctcgg ccctgccgcc attctcggcg cgcgccgcat cgtgcaatcc
     2041 atcgtggacg cctcgactgc gcccgccaag cgcgccagct accgtcgtgt actggagacg
     2101 gagatcgaag aggacgccgc gcgccgcgcc gctcaatgcg acgagaaccc cggcctctgg
     2161 ctgcgcctcc acccgaacgt cagatcgctg gtcctgccgg atgttttcca agcctgaacc
     2221 gtagccgcaa ggcaagaaag ttagaagaat aatgtcgagc atgattacac aggcgcagcc
     2281 cgtcagcgag tacgccatca tcgtcagccc cgaagacaac ctcgcggtcg tcaagcgcga
     2341 aaccgcgccg ggactcgaac tcgctttgcc gggcgggcgc gtcatacgcg tcgcggccac
     2401 agtccccccc ggtcatcgct tcgccacgcg tgacatcccg cagggagagt tcgtcctcca
     2461 atacggccag cccatcggca cgagcctcgg catcacggag ggcgattaca tctcgcacgc
     2521 caacatgtcg gacgacgtgc ccatcgtgcg cgacctgccc gaagacctac agactccgcc
     2581 gcccgactat ctccccgtct ccgagcgcgc cacctttaag ggcttccgcc gacccgacgg
     2641 acgcgtcggc acgcgcaact tcgtactcat cgtgccgacc tctatgtgcg ccagccacga
     2701 ggcgcagcag atttcgatga tgtcggagtt tctacactac aagcgcgagc gttacccgaa
     2761 cgttgacggc gtggtcgcca tcccgcacaa caagggctgc gggtgccagg acggctccac
     2821 catagacgtg atgctgcgca cgctctccaa ctacgccgac cacccgaacg tcggcggcgt
     2881 gatcctgatg gacttgggct gcgagaagac caatctcgcc aaggtcgagc agtacttgct
     2941 caagcgcgat aagaatttcg acaagcccgt cgccaagatc ggcatacagg acgtgggcgg
     3001 cacgcaggcg gccatcgagc agggactcag ggaagtccag cggatgctgc cggaagtgaa
     3061 ccgcatcgag cgcgaagagg tttcggtcag cgaactcgtc ctcggcgtca agtgcggagg
     3121 ctctgacggc ttctccggca tctcggccaa cccctctctg gggcgcgcgg cagaccacct
     3181 cgtacgctcc ggcggcacgg tcttaattac agaggtgccc gagttctgcg gagccgagca
     3241 catcctcgcc aaccgcgcga aggacgctgc gacgggacgc gccatctacc gcatgatcga
     3301 ctggtacaag gagtatgctt cgaagttcgg ggcggtgctc aaccagaacc ccagccccgg
     3361 caacatcgcg ggcggactgc tcaacatcac catcaaatct ctgggcgcga tggcgaaggc
     3421 cgggaccacg cgggtcgaag gcactgtgga ttacgcggaa acgccgcagg gtcgcgggct
     3481 atacttgatg cagggtccgg gctacgatca ggagtctacg ccgggcttgg tcgcagcggg
     3541 cgcaaacgtc gtggtcttca ccacggggcg cggcacgacc atcggtaacg ccatcgtgcc
     3601 cgtcatcaag ctggcttcca acaacgtcgt cttcgagcgc atgtcgcgcg atctagacct
     3661 ctcagccggg aacgtcatcg agggcacgga gacgattgac gaggtcggcg cgcgcgtctt
     3721 cgaacacgtc cggcgggtgg cgggcggcga ggtgctggcg aaggccgaag agcacaagca
     3781 cagggagttt cagttctggg cagagcagac cgtctccctc taaacgatga aggatgaacg
     3841 cggaacgatg aattaggaat tgtcagtggt cagttgcccg ttgcatgtga caattgcaca
     3901 agttactaaa gacgggtttt gaacaactgg caacggacag agaacaacgg gcggctttta
     3961 cttcatcgtt catcgttcat cgttccgcgt tcatcatttc ttaactgata tgcccacgct
     4021 gagagatgta gcaacactgg ccggtgtctc ggtcacgacc gtctcgaacg tcgtcaacga
     4081 cgcacgccgc gtcttaccgg ccacgcgcgc ccgcgtcgag gaagcgatgg cgcaactcaa
     4141 ctatgcgccc gcgcgcggag ttgccgggac aagcgcatct ccgcttcccg ccgacggcca
     4201 cacacgcccc actgcatcct ctttcgcccc cgccattcag gatagatcgc gtacgggcga
     4261 cacaacgggt ttcagggcat acgccgtcgc agccgccgcc gaccccggca cgccgggcaa
     4321 ggcagcccgc atgctcctgc gtctactgcg tgcggcgcag cccatctcac gggtcgaact
     4381 cgcacgccgc ctcggcgtta accgcagcac tgtaaccgac atcttcaaac cgctcatcgc
     4441 ctctggattg gtgcgcgaag agttgggcgc gggcacggat cgcgtcctcg gacgcccgcc
     4501 cgtagctctc tccttcaacg acgatcacgt cttcttcgcc ggtgtcaata tcggcatccg
     4561 gcgcgtgcag gtcggactcg gcacgctcgg cggtgagatc gtgtatgagg acgacttcga
     4621 cacgccccac gatccggcgg aagcgttgtc gctcgtacgt gagcgcatcg ctcgactctg
     4681 cgcagggggc gtgggacgca cgttgcgtat gatcggcgtc agcgtctcag gcatgaccga
     4741 cggaggacgg agccgactcg tctacgcacc tcatctcggc tggcacgacg taaggatcgc
     4801 cgatgccctc cgcttcaatc cggcgggcgg cgtggccgcg ccggacgaag aggccgtgcc
     4861 ggtggtggtc gaaaacgacg ccacggcggc ggcgatctat gagtcccggc tcggcctgcg
     4921 cgacagggac gtgaacctga tggacaactt cgtgctcgtg aggtcgggca cagggatcgg
     4981 cgtcgggctg gtgctcggcg gcgaggtcta caggggcgcg gggcgcggcg aaggcttggc
     5041 gggcgagttc gggcacatga ctatcgtagc cggggggaag gcgtgcgtgt gcggcaatcg
     5101 cggctgctgg gagcgttacg cgtccgcctc gtctgccgcc tcgctctacg caggagatag
     5161 actccagacg aacggggctg cgcgcccgcg ttacgtcgag atcgtggcgc gcgccgaagc
     5221 gggcgaactg cgcgcgcgtc ggacgctgga acacgtcggc gaatatctcg gcatcggcat
     5281 cggcaacgtc atcatgggac tcggcgtgcc gcgcgtgatc ttgagcggtc gcgtcgtact
     5341 cggctggaag ttcatccacg aaccgctccg caacgccgtc gcgcagagca tggcggggag
     5401 ggctggcggc tggtctgtcg agccggggga accgcgcggc gcgggactcg gcggcgcgct
     5461 cgaagtcgcc gccgaaggt
//
LOCUS       DASWNS010000065         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_418124, whole genome shotgun sequence.
ACCESSION   DASWNS010000065 DASWNS010000000
VERSION     DASWNS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418124"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VGB76_02315"
     CDS             <1..611
                     /locus_tag="VGB76_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY0097763.1"
                     /translation="AAGVGCISCHTEHRGADFRPGVAPFNASFQKGVAPEQTCAGCHN
                     DGNKKLYNGREVRTPHGGTFGYPVVSGQWKWKGLDAEAWQQKPDTLKQLVANWPVAPG
                     DENARRSAQFHALHLYRVRTTGSLDGNAEGEVSCSTCHKSYGATLDRETPRTTCAACH
                     NGKTDTRTGVALLSAETPNCTSCHVQHVRDPRHWNPSLLATK"
     gene            684..>1539
                     /locus_tag="VGB76_02320"
     CDS             684..>1539
                     /locus_tag="VGB76_02320"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0097764.1"
                     /translation="MKKSQLFIILALTALLLSINLLTRDDFAGKSYFGGLPWFGWVGV
                     SLFLIAVGIGFALRDAARARRLLEEPPVATKDEEKERLQLTQELLEKYDPDGPNYPHP
                     VIVTERCIGCHACVDACPHDVLAIVNGFATPIASAQCMEDTSCQVECPVNPKACIVVN
                     TTKKIPPRKVPTRDASFMTNVPGCYIIGDVSGTPLIKNAANEGADCIKHIVEELHAAT
                     AEPKAEVDVAIIGAGPAGLSAAVIAKQAGLSYVNIEQDKILATIDAYPANKYVFFKPE
                     TMESRGGIA"
BASE COUNT          326 a          495 c          456 g          262 t
ORIGIN      
        1 tcgccgccgg agtcggctgc atcagttgcc acacggagca tcgcggcgca gacttccgcc
       61 ccggcgtcgc ccccttcaat gcaagttttc aaaagggcgt cgcgccggag cagacctgcg
      121 ccggttgtca taacgacggc aacaaaaaac tctataacgg tcgcgaggtg cgcacgccgc
      181 acgggggcac gttcggctat ccggtcgtga gcggccagtg gaaatggaag ggactcgacg
      241 cggaagcgtg gcagcagaag ccagacaccc tcaagcaact cgtcgcaaac tggccggtcg
      301 cgcccggcga tgagaacgcg cgacgcagcg cgcagtttca cgcgctacat ctctatcgtg
      361 tgcgcacgac cgggagttta gacggaaacg cggaaggcga agtctcctgc tcgacctgtc
      421 ataaatccta cggcgcgacg ctcgaccgcg agactccgcg cacaacctgc gccgcctgtc
      481 ataacggcaa gacggacacg cgcacaggcg tcgcgctgct ctcagccgag acgcccaact
      541 gcacctcttg ccacgtccag cacgtgcgcg acccgcgcca ctggaacccc tcgctgctgg
      601 cgacgaagtg agagccgggc ggcgaagtaa aagtagtctg tagctcaaac ccgcataatt
      661 ttttcgagaa tcttttcgag actatgaaaa agtcgcaact gttcatcatt ctcgcgctga
      721 ccgcgttgct gctgtcgatc aatctgctga cccgcgatga tttcgcgggc aagagctatt
      781 tcggcgggct gccctggttc ggttgggttg gcgtgagcct gtttttgatc gcggtcggca
      841 tcggctttgc gttgcgcgac gcggcgcgtg cgcgccgcct gctcgaagag ccgcccgtcg
      901 ccacgaagga cgaagagaaa gaacgcctcc aactcacgca agagctgctt gagaagtacg
      961 acccagatgg ccctaactac ccgcaccccg tcatcgtcac ggaacgctgc atcggctgtc
     1021 acgcctgcgt agatgcgtgc ccgcatgacg tgctcgccat cgtcaacggt ttcgccaccc
     1081 ccattgcgag cgcgcagtgc atggaagaca cttcctgtca ggtcgaatgc ccggtcaacc
     1141 cgaaggcgtg catcgtcgtc aacacgacga agaagattcc gccgcgcaag gtgccgacgc
     1201 gcgacgcttc gtttatgacg aacgttcccg gctgctacat catcggcgac gtatccggca
     1261 cgcctctcat caagaacgcc gccaacgaag gcgcggattg catcaagcac atcgtcgaag
     1321 aactgcacgc cgcgacagcc gaaccgaaag ccgaggtgga cgtggcgatc atcggcgcag
     1381 gtccggcggg actctccgcc gcagtcatcg cgaaacaggc cgggctttct tacgtgaaca
     1441 tcgaacagga caagatactg gcgaccattg atgcttaccc ggccaacaag tatgtcttct
     1501 tcaaaccgga gacgatggag tcgcgcggcg gcatcgcgg
//
LOCUS       DASWNS010000066        10713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_443937, whole genome shotgun sequence.
ACCESSION   DASWNS010000066 DASWNS010000000
VERSION     DASWNS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443937"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2052
                     /locus_tag="VGB76_02325"
     CDS             <1..2052
                     /locus_tag="VGB76_02325"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0097765.1"
                     /translation="ELHGNKWYAGQALRTLGRCHLASDEPERALAEGLRALRYAEQIG
                     DRHAVCDSRLLLSETHLRRGDLAQCEEQLNLVLDETADSSADLAVSGEAQRLQGLLSL
                     AEGNALPAAQHFGRSVSIYEMLGDRYRSARSLYDLGRAYAAAQPERAQECLNRAAETF
                     RELGARLDITRTEVALAALERSRSSVGDDTARASQQKFDDLTHLILLRLTDAVASREL
                     LLRELAAVIYQETGVRQILITEPGEIAHDSIVVAHGWTEREGEELLEKLRRAESAEER
                     SGVAHAHNTEIIELHAANARPATLLVSPRGAAHLPGGVSLAPLLRMVELGLDVCAFRA
                     KAREGAGGAEQTANPTQSFMPGFIHSSPAMTSLVEEVHKIRSSDVTVLVTGESGTGKE
                     LVARAIHALSSRREKVFVPFNCTAVPKELSDAYLFGYRRGAFTGAVSDSPGVIRSAAG
                     GTLFLDEIGDLPLDIQPKLLRFLQEGEIQPLGEQRPAKVDVRIIAATNSDLDEMVKDG
                     RFREDLYYRLNVIRLRVPPLRERRSEIPTLVNHYIGHYAAKFGRRDIRITPETIDLLM
                     VCEWPGNIRQLVNEIQRIMARAEDATLITPDHLSPELKHTSAPVSTTTTNVALFGSHA
                     NHAPEHLSLPDAVEDLERRMIADALRRHRGNISRAARELGITRRGLQLKLGRYAMSAT
                     S"
     gene            2168..2350
                     /locus_tag="VGB76_02330"
     CDS             2168..2350
                     /locus_tag="VGB76_02330"
                     /inference="COORDINATES: protein motif:HMM:NF033738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microviridin/marinostatin family tricyclic
                     proteinase inhibitor"
                     /protein_id="HEY0097766.1"
                     /translation="MEPTEKANTHDPAEGSETPFFARFLEGQHAETKPDAANDEASFM
                     TLKYPSDRDEIDRQNV"
     gene            2506..3489
                     /locus_tag="VGB76_02335"
     CDS             2506..3489
                     /locus_tag="VGB76_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvdC family ATP-grasp ribosomal peptide
                     maturase"
                     /protein_id="HEY0097767.1"
                     /translation="MQTKPERDTVLLLTHSGDYYTVERVADALAARGVKTFRFDTDRF
                     PREVRLAARLDAEGFSYTIEEAAAVCRADAVRAVWARKIWLPELDEKLDPKFRAMCVR
                     ESVAALHGFLDGLQGARWVNERSRESEAENKLLQLRLAHEAGLRIPRTLLTNDARQAR
                     EFFRQNDGALVAKLLAPLNVSMGASSAFVYTNEVCAADLEEAGGLRHSPMVFQERIPK
                     SIELRIAYVAGKFFVGALDASRSVGGQVDWRRATPAECQWQRDEIPPELGTQLTTLMR
                     KLQLVYGAIDIIRTPAGEHVFLEVNPGGEWGMLERDLDLPISEAIADALLA"
     gene            3516..4514
                     /locus_tag="VGB76_02340"
     CDS             3516..4514
                     /locus_tag="VGB76_02340"
                     /inference="COORDINATES: protein motif:HMM:NF044697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097768.1"
                     /translation="MTVLIITHSKDNESVALVAKAIEQRGGSLFRFDTDRFPTEVRLI
                     AEYGDASERLSLVSGGATLDLRRDVSAIWHRRLNIGGQLPRELDGQMRAASVGEARAT
                     VMGMLASLDAFRMDEELLIRRAENKQLQLQLARAIGLETPRTLITNDPQAVRDFARTC
                     ERGLVAKMLSSFAIQAGDAEKVVFTNPLTAEDLNDLDGLRLCPMTFQEQVSKRLELRV
                     TIIGERVLAAAIDSQASARAAHDWRRDGVQLLDAWQPYQLPSGTQEKLLRLMDYFGLN
                     YGAADFILTPEGRHVFLEVNPVGEFFWLEKCPGLPISDAIADLLLGRSTRRGEGKG"
     gene            complement(4604..5167)
                     /locus_tag="VGB76_02345"
     CDS             complement(4604..5167)
                     /locus_tag="VGB76_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097769.1"
                     /translation="MKEIYVSTDVEADGPIPGPHSMLSFGSAAYLADKTLIGTFTANL
                     EMLPGASGHPETLAWWKTQPEAWAACREDLQTPAEAMKNYVAWLKSLPGRPIFVAYPV
                     VFDYMFVQWYLHKFAGESPFSFIGLDIKSYAMAMLKKDFHHTVKSNMPRHWFDDLPHT
                     HKALEDAIEQGALFCNMLAENTRQEKG"
     gene            complement(5346..6602)
                     /locus_tag="VGB76_02350"
     CDS             complement(5346..6602)
                     /locus_tag="VGB76_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097770.1"
                     /translation="MKRVGILVGREKTFPQALIDRINARGAGEVEAEYVIVGGVTLEE
                     SRRYDIIIDRISHEVPFYRAFLKRAALEGTQIINNPFWWSADDKFFNFSLATKLGVAI
                     PKTALLPQKDYIEGITSESLRNLEFPLDWQGIVDHVGMPAIIKPFDGGGWKNVSRVNS
                     IEELWEVYDTTGTLCMTLQEFIAFDQFVRCYCIGQEEVMIMPYDPRKPYLSGEQYIQD
                     PNYLTPELAERITNDVRTLCRALGYDINTVEFAIKDGIPYAIDFMNPAPDAELASVGE
                     FYHNWVTDTVVNLVFKRLAEPQTEVRYRWDAMLNPRPLDTVETPAQAMSAVASAVGQA
                     VPASIPESGRGIAADIAAGVTDIVEGATNAVSEFLGRTGEAEAAPEENAKPVRAASTK
                     TPKAASARKGAAKSATPRTKKPTPEL"
     gene            6898..7800
                     /locus_tag="VGB76_02355"
     CDS             6898..7800
                     /locus_tag="VGB76_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HEY0097771.1"
                     /translation="MLLVLRRFYIRALAVMVLGAAGLSSPAFAQTTQPGALPTPTPEV
                     KVEKKPELVTGKEAKMPTAEQIAETVILIYGGGGGRAVLDQIRRNGVERGKLTRTASD
                     GRIEESSYEQRFVRGETAGKDKIRVDQKMPTMEYALIFDAGQIWGIINGTAFTPKQEA
                     AAELISQTQHGIDTLLRYKENNSTLAFVGKEKMKGLELWVIDLTTKENNKTRYYISAN
                     PDLRRTARVLWLEYEEPSEAGSPPSKYRRTFHDYRYSQNTLVPFRSVLYKDDKQVQEA
                     RVLTVTYGVKMDDSYFKNPEAASN"
     gene            8154..10100
                     /locus_tag="VGB76_02360"
     CDS             8154..10100
                     /locus_tag="VGB76_02360"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF018571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEY0097772.1"
                     /translation="MPGLNNFEESTLLHALSRNYPTAETAIAELAALRAALSLPKGVV
                     HVVSDVHGEYKKLRHIINNASGSLRELSGQLFGGRLSVEEQQELLAVLYYPRELMEHL
                     HGQLNERAARRVWVRAMLRRQFEIVRELARGCRYRQVSALMPREYAELFEELLAEALT
                     GRTHAYVDEMIDAFAEHSRDLAAVRAASRLVRNLSVSEIIVAGDLGDRGPRLDKVIDY
                     LSEQPNVSFTWGNHDVSWMGACLGQEALIATVLRVSLRYRRLSQLEEGYGLIIAPLER
                     LARTVYAGDPCEHFQTKGTGLRDDHLMAQMQKACAVMQFKLEGQTIRRHPEWEMERRN
                     LLHRIDAKKGTVEIDGRTHTLLDVHLPTLDPADPYALSAEERACMDRLRQSFVSSSRL
                     WEHMSYVSRRGATWLRRDQALIFHGCVPVDERGEFLSLNVEGREYAGRALFDALNTLV
                     RRAINEGAENVGERADWLWYLWTGMRSPLFGKDRMATFENYFVADEEARNEHKNPYFK
                     LIHEAEFCRRVAADFGIEEDALIVNGHVPVKVERGEEPVKRGGNAVTIDGAFSEAYGD
                     RGYTLIIAPERVALAEHHHFESISEAITAGADIVPKVTTIRTYERMRLVADTEEGEVI
                     RRRAGALERLIFAYERGELLEGQK"
     gene            10431..>10713
                     /locus_tag="VGB76_02365"
     CDS             10431..>10713
                     /locus_tag="VGB76_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097773.1"
                     /translation="MEASAVSQQQVQLDTLCVNTIRTLSMDAVQAANSGHPGTPMAMA
                     PVAYLLWNEIMRFDPEDPIWPNRDRFVLSMGHASMLLYSMLHLTGVKAVN"
BASE COUNT         2184 a         3300 c         3154 g         2075 t
ORIGIN      
        1 gagctgcacg gcaataagtg gtacgcggga caagccctgc gcacgctcgg tcgttgtcat
       61 ctcgcgtcgg acgagccgga acgcgcgctc gcagaggggc tgcgcgcgct ccgctatgcg
      121 gagcagatcg gtgatcgcca tgccgtctgc gattcgcgcc tcctgttgag tgagacgcac
      181 ctgcggcgcg gcgacctggc tcaatgcgaa gagcagttaa atctggtgtt ggacgaaacg
      241 gcggactctt cggcagacct cgccgtgtct ggcgaagcac aacgcctgca aggcttgctc
      301 tcgctcgcag agggcaacgc cctgcccgcc gcgcaacact tcgggcgtag cgtctcgatc
      361 tacgaaatgc tcggcgaccg ttatcgcagc gcgcgttcgc tttacgatct cggtcgggct
      421 tacgccgccg cgcagcccga acgcgcacag gaatgtctca accgcgccgc agagactttt
      481 cgcgaactcg gcgcgcggct cgacatcacg cgcacggaag tagctctggc cgcgctcgaa
      541 cgaagccgca gcagcgtcgg cgacgacaca gcccgcgcgt cgcaacaaaa attcgacgac
      601 ctcacgcatc tcatcctgct gcgtctcacc gatgcggtcg cctcccgcga actgctcctg
      661 cgcgaactcg ccgcagtgat ctatcaggag acgggcgtgc ggcaaatcct cattacggag
      721 ccgggtgaga tcgcgcacga cagcatcgtc gtggcgcacg gctggacgga gcgcgagggc
      781 gaagagttgt tggagaagct gcgacgcgcg gagagtgcgg aagagagaag cggcgtcgca
      841 catgcgcaca acacggagat catcgaactg cacgcggcga acgcgcgtcc ggccacgctc
      901 ctcgtttcgc cgcgaggggc ggcgcatctg cccggcggcg tctcgctcgc tccgctcctg
      961 cggatggttg aactcggact cgacgtttgc gcgtttcgcg ccaaggctcg tgagggagcg
     1021 ggcggcgcgg agcagacagc caatccgacc cagagtttca tgcccggctt cattcattcg
     1081 tcgcccgcga tgacgagttt agttgaagag gtgcataaga ttcgctcctc cgatgtgacg
     1141 gtgctcgtga ctggcgaatc aggtacgggc aaggaactgg tcgcacgcgc catccacgcg
     1201 ctctcctcgc gccgcgagaa agtcttcgtg ccgttcaact gcacggcagt cccgaaggaa
     1261 ctctcggacg cctacctgtt cggctatcgc aggggggcgt ttacgggcgc ggtgtccgat
     1321 tctccgggcg tgattcgttc ggcggctggc ggcacactct tcctcgacga gatcggcgac
     1381 ctcccgctcg acatccaacc gaaactcctg cgcttcctgc aagagggcga gattcaaccg
     1441 ctcggcgagc agcgaccggc gaaggtggac gtgcgcatca tcgctgccac caacagcgat
     1501 ctggacgaga tggtgaaaga cggacgcttc cgcgaagacc tctattatcg tctgaacgtt
     1561 atccgcctgc gcgtgccgcc gctccgcgag cgtcgttcag agattcccac gctcgtcaat
     1621 cattacatcg gccactacgc ggcgaagttc ggacgccgcg acatacgcat cacgccggag
     1681 acgattgatc tgctgatggt ctgcgaatgg ccgggcaaca ttcgccaact cgtcaacgag
     1741 attcagcgca tcatggcgcg cgccgaagac gccactttga tcacgcccga ccatctctcg
     1801 cccgaactca aacacacttc cgcgcccgtc tccacgacca cgacaaacgt ggccttattc
     1861 ggctcgcacg ccaatcacgc gccggaacac ctctctctgc ccgacgccgt ggaagacctc
     1921 gaacgccgca tgatcgcaga tgccttgcgc cgccatcgcg gcaacatctc acgcgccgca
     1981 cgcgaactcg gcatcacgcg tcgcggtctt caactcaaac tcggtcgcta cgccatgagc
     2041 gccacctcct gagtcgaaac ttattcctgc cgaccagcgt aaataacacc agcctcgccg
     2101 cagctttgtc ttatcaatac gcgcgtttcc ccatcctctt aatgacagtg ttaaggagaa
     2161 ccccaacatg gaaccgacag aaaaagccaa tacgcacgac ccggcagagg gaagtgaaac
     2221 acctttcttc gcgcgcttcc tcgaagggca gcatgccgaa accaaacctg acgcggcgaa
     2281 cgacgaggct tcattcatga cgctcaagta cccctcagac cgcgacgaga ttgacaggca
     2341 gaacgtttaa tcagaaacgt agccgtcacc acagtgggtc aatctcttaa atgaactgct
     2401 accggagacg ttgacgccac aacaaaggtt aaggacatcc catcccggat gtccttaacc
     2461 tttgcaccgc gactttccca actcttcatc aacagtcttc ttcgtatgca gaccaagccg
     2521 gaacgcgaca ccgtcctgct actcacgcac agcggcgact actacacggt ggaacgcgtc
     2581 gccgacgctc tcgcagcgcg cggcgtaaaa acttttcgct tcgacacaga ccgtttcccg
     2641 cgcgaggtgc ggctcgccgc acgtctcgat gcggaaggct tcagctacac aatcgaagag
     2701 gccgcagccg tatgtcgtgc ggacgcggtg cgcgcggtgt gggcgcgaaa gatttggttg
     2761 ccggagttgg acgaaaagct cgacccgaag tttcgcgcga tgtgcgtgcg cgagtccgtc
     2821 gccgcgctgc atggattcct cgacgggttg cagggcgcgc gttgggtcaa cgaacgcagc
     2881 cgcgagtcgg aggcggaaaa taaactcctg caactgcgcc tcgcacacga agcgggcttg
     2941 cgcatcccgc gcacgctgct gacgaacgac gcgcgacagg cgcgggaatt ctttcgacag
     3001 aacgacggcg cgctcgtggc taaacttctc gctccgctca acgtcagcat gggcgcgtct
     3061 tcggccttcg tctatacgaa cgaagtgtgc gccgccgacc ttgaagaagc gggcgggctt
     3121 cgccacagcc cgatggtctt tcaggagcgc atcccgaaat caatcgaact gcgcatcgca
     3181 tacgtcgccg gaaaattctt tgtcggcgcg ctcgacgcga gccgttcggt gggcgggcaa
     3241 gttgattggc gacgcgccac gcccgcagag tgtcaatggc agcgagacga aattccgccc
     3301 gaactcggaa cgcaactcac gacgttgatg cgcaaactgc aactcgtcta cggcgcgatt
     3361 gatattattc gcacgcctgc gggcgagcac gtctttctcg aagtcaaccc cggcggcgaa
     3421 tggggcatgc tcgaacgcga tctcgatctt cccatctcgg aagccatcgc cgacgccctc
     3481 ctcgcctgag actttcccac ccgcccgaaa cttttatgac cgttctcatc atcacgcaca
     3541 gcaaagataa cgaaagcgtc gcgctcgttg cgaaagctat cgagcagcgc ggcggcagcc
     3601 tctttcgctt cgacacggac agattcccga ccgaagtgcg cctcatcgcc gagtacggcg
     3661 acgcgtccga gagactttcg ctcgtgtccg gcggcgcgac gctcgacctc cggcgcgacg
     3721 tgtcggccat ctggcatcgc cgcctgaaca ttggcgggca actgccgcgc gagttggatg
     3781 gacagatgcg cgcggcgtct gtcggcgaag cccgcgcgac cgtcatggga atgctggcga
     3841 gcctcgatgc gtttcgcatg gacgaagagt tgctgatccg tcgtgccgaa aacaagcagt
     3901 tgcaacttca actcgcacgc gcaatcggat tggagacgcc gcgcacgctc atcaccaacg
     3961 acccgcaagc cgtgcgtgac ttcgcgcgca cgtgcgagcg cggcctcgtc gcaaagatgc
     4021 tctcctcctt cgccatccaa gcgggtgatg cggagaaagt cgtcttcacc aaccctctga
     4081 cggcggaaga cctgaacgat ctcgacggtc tgcgactgtg cccgatgact tttcaggaac
     4141 aggtgtcgaa gcgtctggaa ctacgcgtga cgattatcgg cgagcgcgtg ctggccgccg
     4201 cgattgactc gcaagcgtcc gcacgcgccg cgcacgattg gcgtcgggac ggcgtccaat
     4261 tgctcgacgc atggcagcct taccaactgc cgtccggcac gcaggagaaa ttgttgcgtc
     4321 tgatggacta cttcgggttg aactacgggg cagccgattt catcttgacg ccggaaggcc
     4381 gccacgtctt tctcgaagtc aaccccgtcg gcgagttctt ctggctcgaa aaatgtcccg
     4441 gactgccgat ctcagacgcc atcgccgact tgctgctcgg acgttcgacg cgaaggggag
     4501 agggcaaggg ataaggggca gaggcgaaga cagaaacagg aagcggggcg cagcacttaa
     4561 acgctgcgcc ccgctttcat ctacaaccct gaccctcgac cccttaccct ttctcctgtc
     4621 ttgtgttctc cgcgagcatg ttgcagaaga gcgcgccctg ttcgatggcg tcttccagtg
     4681 ctttgtgcgt gtgcggcaga tcatcgaacc agtgtctggg catgtttgat ttaaccgtgt
     4741 ggtggaaatc ttttttgagc atcgccatcg cataggattt gatgtcgagg ccgatgaaag
     4801 agaacggact ctcgcccgcg aacttgtgca agtaccattg cacgaacatg taatcgaaga
     4861 cgaccggata ggccacgaag atgggacgac cgggcagcga cttgagccat gcgacgtagt
     4921 tcttcatggc ctctgcgggc gtctgtaaat cttcgcggca ggccgcccat gcttcgggct
     4981 gcgtcttcca ccacgcgagc gtttcgggat ggccgctcgc gccgggcagc atttcgaggt
     5041 tggccgtgaa cgtgccgatc aaagtcttgt cggcgagata agcagcggag ccgaaactga
     5101 gcatcgaatg agggccggga atcgggccat cggcttccac gtcggtgctg acgtaaattt
     5161 ccttcatgtt tttaacctcg atgcgaattg aaaatagctg accgaaccgg cacgcgcctt
     5221 tccgcgcacg cgtttgcgga tgacgacaaa cgacgacaaa ctaagagacg gtcgcattcc
     5281 gttgcgaccg tctctcattt cgagtcaatt caagactgtc cgttctgtcc gttaaagttc
     5341 ggcggttaaa gttccggcgt cggcttcttc gtgcgcggcg tggcggattt cgctgccccc
     5401 ttacgcgctg atgcggcttt cggagttttc gtcgaagcgg cgcgcacggg cttcgcgttc
     5461 tcctccgggg cagcttccgc ttcaccagtc ctgccgagaa attccgacac ggcgttggtc
     5521 gcgccttcga cgatgtctgt cacgcccgcc gcgatgtcgg cagcgatccc acgtcccgat
     5581 tcgggaatgc tcgcgggcac agcctgcccg accgcagagg cgaccgccga catcgcctgc
     5641 gccggtgtct cgacagtgtc gagggggcgc gggttcagca tcgcatccca gcgataacga
     5701 acttcggtct gcggctcggc gaggcgtttg aagacgagat tgacgaccgt atccgtcacc
     5761 cagttgtgat aaaactcgcc gaccgaagcg agttcggcat cgggcgcggg gttcatgaaa
     5821 tcaatggcgt aggggatgcc gtccttgatc gcaaactcga ccgtattgat gtcgtagccg
     5881 agtgcgcggc agagcgtgcg cacgtcgttc gtgatgcgct cggcgagttc gggcgtcagg
     5941 tagttcgggt cttgaatata ctgctcgccg gagagataag gcttgcgcgg atcgtagggc
     6001 atgatcatca cttcttcctg cccgatgcag tagcagcgca caaactggtc gaaggcgatg
     6061 aactcctgga gcgtcatgca gagcgtcccc gtcgtgtcgt aaacttccca gagttcctct
     6121 atcgaattga cgcgtgagac gtttttccag ccgccgccgt cgaagggctt gatgatggcg
     6181 ggcatgccta cgtgatccac gatgccttgc cagtcgagcg gaaattcgag attgcgcaag
     6241 gactcgctcg tgatgccttc gatgtagtct ttctgcggca ggagcgcggt tttcgggatg
     6301 gcgacgccga gcttggtcgc gagcgaaaag ttgaaaaatt tatcgtcggc tgaccaccag
     6361 aaagggttat tgatgatctg cgtgccttcg agcgcggcgc gcttcaggaa cgcgcggtag
     6421 aagggaactt catgcgaaat gcggtcaata ataatgtcgt agcggcgcga ttcttcgagc
     6481 gtcacgccgc cgacgatcac gtactcggct tcaacttcgc ccgccccgcg cgcgttaatc
     6541 cggtcaatca atgcctgcgg aaacgtcttc tcacggccaa ccaaaatacc aactctcttc
     6601 acaatgttaa tatcctttca aaaaaatatc gtgcagccgg cgacgggcgg aaaagttaag
     6661 acctttgtta cctgtcgcca ctcacgcgtc tatgaaaacc gtgaactcta catgcccccc
     6721 gccatgttgt caaatgagcg gcgggcatcc gcacgcaggg ttttacgcat caataaaagt
     6781 gctcggcgct gacccgggcg cgacggctta cgggcgtcgg cttctatgtt acaattcgcc
     6841 aattgatttg ttcgatcttc actcccgatg gtttttagcg aaaaagtttt taaggagatg
     6901 ttactggtgt tgcgacgttt ttatatacgt gcgttagccg tcatggtgct tggtgcggct
     6961 ggcctctcca gccccgcttt tgctcaaaca actcaacccg gcgcgttgcc gaccccgacg
     7021 cccgaagtga aggttgaaaa aaaacctgaa ctggttaccg gcaaagaagc caaaatgccg
     7081 accgccgagc agatagccga gacggtcatc ctcatctacg gcggcggcgg cggacgcgcc
     7141 gtgctcgatc agatcaggcg caacggcgtc gagcgcggga aactgacgcg cacggcgagc
     7201 gacgggcgca tagaggaatc cagctacgag caacgcttcg tacgcggcga gacggcgggc
     7261 aaggataaga ttcgcgtcga ccaaaagatg cccacgatgg aatacgcgct catcttcgac
     7321 gcggggcaaa tctggggcat catcaacggc acggccttca cgccgaagca ggaagccgcc
     7381 gccgaactca tctcgcagac ccagcacggc atagacacgc tgctgcgtta taaagagaac
     7441 aactcgacgc tggccttcgt cgggaaagag aagatgaagg ggcttgaact ctgggttatc
     7501 gacctcacca ccaaagaaaa caacaagacg cgctactaca tcagcgcgaa ccccgacctg
     7561 cgccgcacgg cgcgcgtcct ctggctcgaa tacgaggagc cgtcggaggc cggttcgccg
     7621 cccagcaagt accgtcggac tttccacgat tatcgctatt cgcaaaacac gctcgtgccc
     7681 ttccgctccg tcctctacaa agatgataag caggtgcagg aggcgcgcgt gctcaccgtc
     7741 acctacggcg tcaagatgga cgacagctac ttcaagaatc cggaagcggc gtccaactaa
     7801 gccgggcggg cgtcgcccgg ttctccaaca cgaatatttt gtgagaggct gaaattgatc
     7861 gtttcagcct ctcatttttt ggcgacggac gcttgcgaat tgctacgtga attacgctgc
     7921 gagttgctca ggcgggcgcg aaatgctcgc gggttgaggt tgtggcttta gacaaaaggg
     7981 tgtgctagta tcccgccgct ttcgccccga aacttcgtcc gcttgagccg gtgtttgccc
     8041 ttaagctttc cttcaaactt gaaactccgt ctgccgcgtt tccgagccgt tgccttcacc
     8101 ttgcccgccc ctcgacgcgc ccgcgcgcaa acttacacag gaggagcctc ttcttgcccg
     8161 gcctgaataa ttttgaggag tccacgctac tccacgcgct ctcccgcaac tacccgacgg
     8221 ctgagacggc cattgccgaa ctggccgctt tgcgcgccgc cctctcgctc ccgaaaggcg
     8281 tcgtccacgt cgtcagcgat gtccacggcg agtataaaaa acttcgtcac atcatcaaca
     8341 acgcgtcggg cagtttgcgc gaactctcag gccaactctt cggcggcagg ttgagcgtcg
     8401 aagagcaaca ggaattattg gccgtcctct attacccgcg tgaattgatg gagcacctgc
     8461 acgggcagtt gaacgaacgc gcggcgcgtc gtgtctgggt gcgcgcgatg ctccggcggc
     8521 aattcgagat cgtgcgcgaa ctggcgcgcg ggtgtcgtta ccgtcaagtc tccgccctga
     8581 tgccgcgtga atacgcagag cttttcgagg aactgctcgc agaggctttg acgggacgca
     8641 cgcacgccta cgtggacgag atgatcgacg ccttcgccga acacagccgc gaccttgcgg
     8701 cggtgcgcgc ggcttcgcgt ctggtgcgca atttatccgt ctccgagatc atcgtggcgg
     8761 gcgacctcgg cgaccgtgga ccgcgtctcg acaaagtgat cgattatctc tcggagcagc
     8821 cgaacgtctc tttcacttgg ggcaatcacg atgtctcctg gatgggcgcg tgcctggggc
     8881 aggaagccct gatcgcgacg gtgctccgcg tctcgctccg ctatcgccgc ctgtcgcaac
     8941 tcgaagaagg ctacggcctg atcatcgcgc cgctcgaacg tctggcgcgg acagtctacg
     9001 cgggcgaccc ttgcgagcat tttcagacga agggcaccgg cttgcgcgac gatcacttga
     9061 tggcgcagat gcaaaaagcc tgcgccgtca tgcagttcaa actcgaaggt cagaccatcc
     9121 ggcgccatcc cgaatgggag atggaacggc gcaatctgtt gcaccggatt gacgcgaaaa
     9181 aagggacggt cgaaattgac gggcgcacgc acacgctgct cgatgtgcat ctgccgaccc
     9241 ttgacccggc tgacccttac gcgctctccg cagaagagcg cgcgtgcatg gatcgcctgc
     9301 gacaatcgtt cgtttcgagt tcgcgcctgt gggagcacat gtcttatgtc tcgcgtcgcg
     9361 gcgcgacgtg gctccgcagg gatcaggctt tgatctttca cggctgcgtg ccggttgacg
     9421 agcgcggcga gtttctctct ctgaacgtag aggggcggga gtatgcgggg cgcgcgttgt
     9481 tcgatgcgct caatacgctg gtgcgccgcg cgataaacga gggcgcggag aacgtgggcg
     9541 agcgcgccga ttggctctgg tatctctgga cgggcatgcg ctcgccgctc ttcggtaaag
     9601 atcgcatggc gacttttgaa aactattttg tcgcagacga agaggcgcgc aacgagcaca
     9661 agaatcctta cttcaagttg attcacgaag cggaattctg tcgccgtgta gcggctgatt
     9721 tcggcatcga ggaagacgct ctgatcgtca acggtcacgt ccccgttaag gtcgagagag
     9781 gcgaagaacc cgtcaagcgc ggcggcaacg ccgtcacgat tgacggcgca ttctccgaag
     9841 cctacggcga ccgtggctat acgctcatca tcgcgcccga acgcgtcgcg ctcgcagagc
     9901 atcatcactt cgaatccatc tcggaggcga tcaccgccgg ggcagacatc gtgccgaagg
     9961 tcacgaccat tcgcacctac gagcgcatgc gcctcgtcgc agacacggaa gaaggcgaag
    10021 ttatccgccg ccgcgcaggc gcgctcgaac gactgatctt cgcctacgag cgcggcgaat
    10081 tgttggaagg gcaaaaatga aagctgagtt gcgcgtgaag cgcacggcgc ggcagaagtg
    10141 catagacgtt ccttccacaa ctgtgttagt atcccgccgc ttcaacgggc gacaccaaag
    10201 tcttttcgcg tcctgctttt catcctgatt agggttaggc tttccgcgca ctgtcctaca
    10261 ggttccaatt gtgatctctg aaaataatct gcgaagatgc tttaaacgtc ttacatgatc
    10321 acagacgggg caacgctctt tccgcacgtc acaaccgcta caatgtgcgt ttgcacggcc
    10381 ttccgcgaag ggcgggaggg tagaaaaatt ctgcaaggag ccgacaatta atggaagcat
    10441 ccgctgtgag ccaacagcag gtacaactcg acactctctg cgttaacacg atccgcacgc
    10501 tctcgatgga cgccgtgcag gcggccaact caggccaccc cggcacgccg atggcgatgg
    10561 ctccggtggc ctacctgctc tggaacgaga tcatgcgctt cgacccggaa gaccccatct
    10621 ggccgaaccg cgaccgcttc gtgctctcga tgggacacgc ctcgatgcta ctctattcga
    10681 tgctgcacct gacgggcgtg aaggccgtca atg
//
LOCUS       DASWNS010000067         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_132245, whole genome shotgun sequence.
ACCESSION   DASWNS010000067 DASWNS010000000
VERSION     DASWNS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132245"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VGB76_02370"
     CDS             <1..837
                     /locus_tag="VGB76_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0097774.1"
                     /translation="ELAAVVIATPVATHYEFARAALAAGKHVLIEKPFTTNSREAEEL
                     VALAQARGLTLMVDHTFVYTGAVRRIKEIVESGELGELLYFDSVRINLGLFQRDINVV
                     WDLAPHDLSIMDYLVEREPVALTATGSSHIEAGIENIAYVMLRFRDSFIAHFHFNWLS
                     PVKIRRTLIAGSRKMIVYDDIEPTEKVRVYDKGVTTNRIESDTDKEAAYRTLVSYRTG
                     DVWVPKLDSTEALTHVSAEFLEAITDKRAPLTDGSAGLRVVRLLEAAQESINQGGKLI
                     EL"
     gene            844..1983
                     /locus_tag="VGB76_02375"
     CDS             844..1983
                     /locus_tag="VGB76_02375"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0097775.1"
                     /translation="MTTTPTTGMQVPLLDLRQQHEALREALRAATLRVLDSQQFVLGE
                     EVRLLEQEIARYCATRHAIGCASGSDALLLASMALDIGAGDEVITTPFSFFATASSIT
                     RTGARPVFVDIEPHTYNLDPSLVEAAITERTRAIMPVHLYGQCAGMDALLEIAARHQL
                     PVIEDAAQAIGAEDATRRAGSMGRIGCFSFYPTKNLGGAGDGGMLTTDDDALAERLRA
                     LRVHGGATEYLHREIGINSRLDALQAAILRVKLPHLDSWSQARRERAETYSLLLTNAR
                     LAFPLSIPFIREHTRHIFHQYVVRVPRHRDALIEHLRAHGVGVKIYYPMPLHLQECFS
                     YLGYEAGALSVAERAAGETLALPLYPELRLEQQQYVVDVISRFRP"
     gene            2036..>3030
                     /locus_tag="VGB76_02380"
     CDS             2036..>3030
                     /locus_tag="VGB76_02380"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0097776.1"
                     /translation="MTKKALITGITGQDGSYLAEHLLSLGYEVHGLVRRVALEDAARR
                     FTRISHLLDRVILHAASLESYPSLFHVVSKHDFDECYHLAAQSFVAESFADGFSTMNT
                     NINGTHYMLAALRELRPDCKFYFAGSSEMFGKVAETPQRETTRFHPRSPYGISKVAGF
                     ELTRNYREAYGMFCASGLLFNHEGPRRGYEFVTRKITSTAARIKLGLAEELRLGNLEA
                     QRDWGHAKDYVRAMHLMLQQGEPDDYVIATGETHTVREFCQRAFGEAGLDYEQYVKVD
                     ERFLRPAEVDLLIGDAGKAKRELGWEPEYTFGEMIKEMVEADMKEAQKECGQSVSA"
BASE COUNT          618 a          971 c          888 g          553 t
ORIGIN      
        1 gaactcgcgg cggtcgtcat cgccacgccc gtcgccacgc attacgagtt tgcacgcgcc
       61 gcgctcgcag cgggcaagca cgtgctcatc gaaaaacctt tcacgacgaa cagccgcgag
      121 gccgaagagt tggtcgcgct ggcgcaggct cgcggcctga ccttgatggt cgatcacacg
      181 ttcgtctata cgggcgcggt gcgccgcatc aaagagatcg tcgagagcgg cgaactcggc
      241 gagttgctct acttcgacag cgtgcgcatc aatctcggcc tcttccagcg cgacatcaac
      301 gtcgtctggg atttagcccc gcacgatctc tcgatcatgg actacctcgt cgagcgcgaa
      361 cccgtcgcgc tcacggccac gggtagctcg cacatcgaag cgggcatcga aaacatcgcc
      421 tacgtgatgc tgcgcttccg cgattccttc atcgcacact ttcatttcaa ctggctctcg
      481 cccgtcaaaa tccgccgcac gctcatcgcc ggaagccgca agatgatcgt ctatgacgac
      541 atcgaaccga ccgagaaagt gcgcgtctac gacaagggcg tgacgaccaa tcgcatcgaa
      601 tccgacaccg acaaggaggc cgcttaccgc accctcgtca gctaccgcac gggcgacgtg
      661 tgggttccga agctcgattc gaccgaagcc ttaacgcacg tctccgccga attcctcgaa
      721 gccatcacag acaaacgcgc gccgctcacc gacggctcgg cgggcttgcg cgtcgtgcgc
      781 ctgctcgaag ccgcgcagga atcaatcaat caaggcggca aactgatcga gttataattc
      841 ctgatgacga ccactcccac aacgggcatg caagtgcccc tgctcgacct gagacagcaa
      901 cacgaagcgt tgcgcgaagc gttgcgcgcg gcgaccttgc gcgtcctcga ttcgcaacag
      961 ttcgtcttag gcgaagaagt ccggctgctc gaacaggaga tcgcgcgcta ttgcgcgacg
     1021 cgccacgcca tcggctgcgc ttccggttcc gacgcgctcc tgctcgccag catggcctta
     1081 gacataggcg cgggcgacga agttatcact acgccgttca gtttcttcgc caccgccagt
     1141 tcgatcacgc gaacgggcgc gcgccccgtc ttcgtggaca tcgaaccgca cacttacaac
     1201 ctcgacccgt cgctcgtcga agccgccatc acggagcgca cgcgcgccat catgcccgtg
     1261 catctctacg gccagtgcgc cgggatggac gcgctgttgg agatcgccgc gcggcaccaa
     1321 ctccccgtca tcgaagatgc ggcgcaggcc atcggcgcag aagatgccac gcgccgcgcg
     1381 gggtctatgg gacgcatcgg ttgtttcagt ttctacccga cgaaaaatct cggcggcgcg
     1441 ggcgacggcg gcatgctcac gactgacgac gacgcgcttg ccgaacgctt gcgcgccttg
     1501 cgcgtccacg gcggcgcaac cgaatatcta catcgagaga tcggcatcaa cagccgtctc
     1561 gacgccttgc aggcagcgat cctgcgcgtc aagctgccgc atctcgatag ttggtcgcag
     1621 gcgcggcgcg aacgcgcaga gacttactcg ctgctcttga cgaacgcgcg ccttgctttc
     1681 cctctgagca ttcccttcat ccgcgaacac acgcgacaca tctttcacca gtacgtcgtg
     1741 cgcgtgccaa ggcatcgtga cgcgctcatc gaacacttgc gcgcacacgg cgtcggcgtc
     1801 aaaatttact atcctatgcc gctgcatttg caggagtgtt tcagctatct cggctacgag
     1861 gcgggcgcgc tctccgtcgc ggaacgcgct gcgggcgaaa cgctcgccct gccgctctac
     1921 cctgaactgc ggctcgaaca acagcaatac gtggttgacg taatcagtcg ctttcgtccc
     1981 tgacgacaag cgactgaaac gcacacccgc gctcttattt tcggagactc aagcattgac
     2041 gaagaaggca ttaatcaccg gcatcaccgg acaggacggc agctacttag ccgaacatct
     2101 cctctcgctc ggctacgaag tccacggact cgtccgccgc gtcgccctcg aagacgccgc
     2161 ccgccgcttt acccgcatct cgcatctctt agaccgcgtc atcctccatg ccgcctcttt
     2221 ggagagctat ccttcgctct tccacgtcgt ctcaaaacac gacttcgacg agtgctatca
     2281 cctcgccgca cagagtttcg tcgccgaatc tttcgccgac ggcttctcca cgatgaacac
     2341 caacatcaac ggcacgcact acatgctggc cgcactccga gagttgcgcc ccgactgtaa
     2401 gttctacttc gccgggtcga gtgagatgtt cggcaaggtg gccgagacgc cgcagcggga
     2461 gacgacgcgc tttcatcccc gctcgcccta cggcatctcg aaagtggcgg gctttgagtt
     2521 gacgagaaat tatcgtgaag cctacgggat gttctgcgcc agcggattgt tgttcaacca
     2581 tgaggggcca cgccggggct acgagttcgt gacgagaaag atcacctcga cggcggcgcg
     2641 catcaagctg gggctggcgg aggaactacg cttaggcaac cttgaggcgc agcgcgactg
     2701 ggggcacgcc aaagactatg tgcgagcgat gcacctgatg ctgcaacagg gagagcctga
     2761 cgattatgtg atcgcgacgg gagagacgca cacggtcagg gagttctgcc agcgtgcgtt
     2821 tggagaagcg ggattggatt atgagcaata tgtgaaagtg gacgagcgat ttctgcgacc
     2881 ggcggaagta gatttgctga tcggggatgc ggggaaggcg aagcgggagc tagggtggga
     2941 gccggagtat acgtttgggg agatgatcaa ggagatggtg gaggcggaca tgaaggaggc
     3001 gcagaaggag tgcggccaga gtgtctccgc
//
LOCUS       DASWNS010000068         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_185119, whole genome shotgun sequence.
ACCESSION   DASWNS010000068 DASWNS010000000
VERSION     DASWNS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185119"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VGB76_02385"
     CDS             <1..294
                     /locus_tag="VGB76_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY0097777.1"
                     /translation="YIIAADDPRPPAERTGVDERFAQVYNIDYGRCIFCGYCVEACPK
                     DAITHGYNFELSVYNRADLLKTKDDLLITKQKQSSNYRVAFADEDVNSEYKEV"
     gene            486..1220
                     /locus_tag="VGB76_02390"
     CDS             486..1220
                     /locus_tag="VGB76_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0097778.1"
                     /translation="MQRTTDDGQPATNERMNHSLIETREVSKHYQMGATTVVALDRVS
                     VAVEEGEFVTLLGTSGSGKSTLLNLIGGLDHPSTGEVVFDGRSLAPFTKREMARYRRR
                     SVGMIFQNFNLITTMTAAENVELALAFGGLAGREREARASELLRRVGLGERQDHRPSE
                     LSGGEQQRVAIARALANRPRVLLADEPTGNLDSTRARELLSLLREMVSHDKLTVLLVT
                     HDRDLAAQFADRTIQMRDGRVVMSDE"
     gene            1313..2221
                     /locus_tag="VGB76_02395"
     CDS             1313..2221
                     /locus_tag="VGB76_02395"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY0097779.1"
                     /translation="MQKETPHVLTLEALRSVPLFSGLDNAAANTLRGLLSVRDVPAGT
                     LLFKVGDVGDKMFLIEAGRVRIYIHDGDGDDLTLAELAPGDFFGEMAIVDGKPRSAHA
                     AVTEEARLAVLSREDFLNFVRSNVDIALEMVSAISDRLRRTDELLRQRVSRNVNEEDE
                     ARLTWADHVADSLAAFGGSWKFITYSVLFLVAWILLNTWMMSDAGFDPFPYQLLGLVL
                     GVVAGVQAPIIMMSQNRQAEKERLRADLDFQINLKNELAIAEVLRRLDLLESERLPLL
                     FAEQNARFADAKRQSIEAGDGKFPAS"
     gene            2391..3086
                     /locus_tag="VGB76_02400"
     CDS             2391..3086
                     /locus_tag="VGB76_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY0097780.1"
                     /translation="MIKAILFDFNGVVIDDEPLQLKAAQAALEAEGIPLTERDYYDSL
                     GMDDHTFFRAAFARAGREHSEEVFNRVLEAFTKSHRELIEDELPLFPGVVTFIKAVSR
                     RWPLAVVSMARRASIEYVLERAGLAEAFAVVVSAEDVSACKPDPACVNRALEKLNEAR
                     RAAGQPALAPAECLVIEDSPPGIEAARTAGMRTLGVTNTVSEQLLRAAGADVVTASLA
                     DWTDDAVRHVFDK"
BASE COUNT          647 a         1011 c          980 g          663 t
ORIGIN      
        1 tacatcatcg cggcggacga cccgcgcccg cctgccgagc gcacgggcgt agacgagcgt
       61 ttcgcacagg tctataacat cgactacggg cgttgcatct tctgcggcta ctgcgtcgaa
      121 gcctgtccga aggacgccat cacgcacggc tataatttcg aactctccgt ctataaccgc
      181 gccgacctgt tgaagaccaa agacgatctg ctcatcacca agcagaagca gtcgtccaac
      241 tatcgcgtcg ccttcgccga cgaagatgtc aactcggaat acaaagaggt gtgagggggc
      301 gcggtttgcc agccgccggt tgcccgttgt atgaagcgag aggcgacttg aattttaaag
      361 ccgtcgcgtg ttatttcgca cgcgacggct ttcccgcctg ccagtagtca aattgccggt
      421 agccgtttgt cgagcggttg tcggtagttc gatttaatct ttagcgacct gctttttttc
      481 agcacatgca acgcacgact gacgacggac aaccggccac gaacgaacgc atgaatcact
      541 ccctgatcga gacgcgcgaa gtttccaagc attaccagat gggcgcgacg acggtggtgg
      601 ccttagaccg cgtttcggtt gcggtggaag aaggcgagtt tgtcacgttg ctgggcacgt
      661 ccgggtctgg gaagtctacg ctcttgaatt tgatcggcgg gctggatcat ccgagcacgg
      721 gcgaggtggt gtttgacggg cgttcgctcg cgccgtttac gaagcgcgag atggcgcgtt
      781 acagacgccg ctcggtcggc atgatctttc agaatttcaa cctcatcacg acgatgaccg
      841 ccgccgaaaa cgtcgaactc gcactcgcct tcggcggcct cgccgggcgc gaaagggaag
      901 ctcgcgcgag cgagttgctc cggcgcgtcg gcctcggcga gcggcaagac catcgcccct
      961 cggaactatc gggcggcgag cagcagcgcg tcgccatcgc ccgcgcgctc gccaatcgcc
     1021 cgcgcgtgct cctcgcagac gagccgaccg gcaatctcga ttcgacccgc gcacgcgaac
     1081 ttctctcgct cctgcgcgag atggtttcgc acgacaaact gaccgtcctc cttgtcacac
     1141 acgaccgcga tctcgccgcc caattcgccg accgcaccat tcagatgaga gacggaagag
     1201 tagtgatgag tgatgagtga tgagtgatga gtaaaaacag aagccccgtc tctctttttc
     1261 actcatcact catcactcat cactcattac ttcactatcg aggtgaatcg ttatgcagaa
     1321 ggaaactccc cacgtattga cgcttgaggc tttgcgttcc gtgccgttgt tcagcggttt
     1381 ggacaacgcg gcggcaaaca ctttgcgcgg tctgctctcc gtgcgcgatg tcccggcggg
     1441 caccctgttg ttcaaggtgg gcgacgtggg cgacaagatg tttttgatcg aggcgggacg
     1501 cgtgcgcatc tacatccacg acggcgacgg cgacgacctg acgctcgcgg aactcgcccc
     1561 cggcgacttc ttcggcgaga tggcgattgt cgatggcaag ccgcgttcgg cgcatgccgc
     1621 cgtcaccgaa gaagcgcggc tcgccgtgct ctcgcgcgaa gattttctca acttcgtgcg
     1681 cagcaacgtg gacatcgcgt tggagatggt gagcgcgatc tccgaccgcc tgcgacgcac
     1741 ggatgaactg ttgcgccagc gcgtctcccg caacgtcaac gaggaggacg aggcgcgtct
     1801 gacgtgggcg gatcatgtgg ccgactcgct cgcggcgttt ggcggcagtt ggaagttcat
     1861 cacctactcg gtgctctttc tggtcgcatg gattctcttg aacacctgga tgatgagcga
     1921 cgcagggttt gatcctttcc cttaccagtt gctcggcctc gtgctcggcg tcgtcgcggg
     1981 tgtgcaggct ccgatcatca tgatgtcgca gaaccggcag gcggagaaag aacgcctgcg
     2041 cgccgatctc gattttcaga tcaacttgaa gaacgaactc gccatagcgg aagtcctgcg
     2101 ccgcctcgac ctgcttgaga gcgaacgcct gcccctgctc ttcgccgaac aaaacgcgcg
     2161 ctttgccgac gcgaaacgac agagcatcga agcgggcgac gggaagtttc cggcgtcctg
     2221 aacctgtcgc acacacggga gacgcgaaac agcgcgtgcc ggattttgga gtttggcgcg
     2281 cagcgtcgta tcttggtcgg gcaagacacg tatttcaaat agcagttacc ggatttaaat
     2341 ttcagggatg agcgcacgag tcttatcccg cgcccgaaat tttcacgcgc atgattaaag
     2401 caattctttt cgacttcaac ggcgtcgtca tcgacgatga accgctgcaa ctcaaggccg
     2461 cgcaagccgc gctcgaagcg gagggcatcc cactcaccga acgcgattac tacgactcgc
     2521 tcggcatgga cgaccacacg ttttttcgcg ccgcctttgc acgcgccgga cgcgaacatt
     2581 ccgaggaagt ttttaaccgc gtgctggaag ccttcacgaa gtcgcatcgc gagttgatcg
     2641 aagacgagtt gccgctcttt ccgggcgtcg tcacgttcat taaagccgtg tcgcgccgtt
     2701 ggccgctcgc cgtcgtcagc atggcgcggc gcgcgagcat cgaatatgtg ctcgaacgcg
     2761 cggggctggc ggaggctttc gcggtcgtcg tcagcgcgga agacgtgagc gcgtgcaagc
     2821 ccgacccggc ctgcgtcaat cgcgcgctcg aaaagttgaa cgaggcgcgc cgcgctgccg
     2881 ggcagcccgc cctcgcgccc gccgaatgtc tcgtcatcga agactcgccg ccgggaatcg
     2941 aagccgcacg caccgccgga atgcgcacgc tcggcgtgac gaacaccgtc agcgaacaac
     3001 tcctgcgcgc cgccggagcc gatgtcgtca ccgcgagcct cgccgactgg acggacgacg
     3061 cggtcagaca cgtgttcgac aaatgatgaa cgatgaatgc ggaatgatga atagagcgaa
     3121 aagcatcccc gctttgattc aacgttcact tttccgcgtt caacattcat ccttccgcat
     3181 tcatcgtttt cttctatgac tcctgccgga aaattgttga tggtggacgc acggcgtcgt
     3241 ttcgcgcggc tcgtggcgca gcccgatgcc tcgattgaac tggcgcacgc ggccttgctc
     3301 a
//
LOCUS       DASWNS010000069         2765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_158156, whole genome shotgun sequence.
ACCESSION   DASWNS010000069 DASWNS010000000
VERSION     DASWNS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158156"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..>2765)
                     /locus_tag="VGB76_02405"
     CDS             complement(194..>2765)
                     /locus_tag="VGB76_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0097781.1"
                     /translation="PRQGEGHVSLMLHTFIGADAASVREKVRQPFINYLRSSFGLIKN
                     LARSLGQDIEGKDFTEDDMQALLARAFDRYFETSGLFGTPESCLQIVERLKAIGVDEV
                     ACLVDFGVDFDSVMSSLNYLSAVKEESNRKDDAAEEDYSLPAQLQRHSVSHLQCTPSM
                     ASMLATQPGALDALRSVRQLLLGGEALPAALAAHLSEELAAEIHNMYGPTETTIWSAT
                     QLLAKDGGSVPIGRPIANTQIYILDRHLQPVPVNVPGELFIGGAGVVRGYLKRPDLSA
                     ERFIPDPFGKEGGARLYRTGDLARYLPGGEIEFIGRIDDQVKVRGHRIELGEIETAIV
                     EHAGVREAVVVAREDVPGDKRLVAYLVPAQARQQDHEQPLTPIDQERILADRRGYRLP
                     NGMVVAHHSGLQTNIIYREVFEDKVYLKQGITLNDGDCIFDVGANIGLFTLFVHQQCK
                     KPVVYAFEPIPPTFDLLSTNVALYGLDVKLFNCGLSGETKTSPFTFYPHAAGLSGYIS
                     GAEEDKQVTKLIIQSWLQKVAPENNGAVLPQAELDALMEEQLQSETHVCQLRTLSEII
                     RENSVERIDLLKIDVEGSEYDVLAGIAPADWKKIRQMVVEVHTGELLDRIKVLLERHG
                     FNLSVDETILVEETNGGAATYVYTLYAIHPSREKAPAETKEIAAGVVPAASAPVELSL
                     GELRDALKDRLPDFMIPTAFVLLDALPLTPNGKVNRRALPPPDAGQAERRTIVDLPRT
                     PTEKALAKVWIEVLGGEEVGIHDDFFESGGHSLTATQFVSRVHALFAVDLTLRSFLKS
                     PTISSLAEMVEEGLIAKSSTDRIDELLDLLETLDEDELETLPDIDLTPNGDRHKNLIA
                     "
BASE COUNT          511 a          812 c          828 g          614 t
ORIGIN      
        1 cctccccttg cgtggcttcc cgctgcaacg ctttcaagag aagcgcgcgt ttggcgggtg
       61 acagtttttt cagccgttcg agccggtcag cttcctgcat cttggatatg ggcttttgca
      121 cggatggcgt gcgccatcat ctacggtttg tgtggctagg gacgaggcta aggacgaggc
      181 tacggctcgc cgttcaggcg atcagatttt tatgcctgtc gccgttgggc gtgagatcga
      241 tgtcgggtaa agtttcgagt tcatcttcgt cgagcgtctc cagtaggtcg agcagttcat
      301 cgatcctgtc agtgctcgat ttggcgatca gaccttcttc caccatctcg gcgagactgc
      361 tgatcgtggg agacttgaga aaacttcgca gcgtcaggtc aacggcaaag agggcgtgca
      421 cccgcgagac gaactgtgtc gccgtcagag agtggccgcc tgactcgaag aagtcgtcat
      481 gtatcccgac ctcttcgcct cccagcactt caatccacac ctttgccagc gccttctccg
      541 tcggtgtgcg cggcagatcg acaatggttc gacgctcggc ctgcccggcg tcgggtggag
      601 gcagcgcgcg gcggttcact ttcccgttag gtgtcagcgg cagcgcatcc aacaacacga
      661 aggctgttgg aatcatgaag tcgggcagtc tatctttgag cgcgtcgcgc aactcgccga
      721 gcgaaagctc gaccggggca gacgcggcgg gcacaacccc ggcggcaatc tctttcgtct
      781 ccgcaggagc tttctccctt gaggggtgga tggcatagag cgtatagacg taggtggcgg
      841 ctcctccgtt cgtctcttcg accagaatgg tttcatctac ggagaggttg aagccgtgcc
      901 tctcaagcag aaccttgatc cggtcgagca gttcgcccgt gtgtacctcg acgaccatct
      961 gcctgatctt tttccagtca gccggagcga taccggcgag cacgtcgtat tcgcttcctt
     1021 ccacgtcgat cttgagcaga tcaatacgct cgacgctgtt ctcgcggatg atctccgaga
     1081 gagttctcaa ctggcagacg tgtgtttcgc tttgaagttg ctcttccatc agagcgtcga
     1141 gttcggcttg cggcaagacc gcgccgttgt tctccggcgc aaccttttgc agccagcttt
     1201 ggatgatcaa cttcgttacc tgcttgtcct cttccgctcc cgaaatgtaa ccggagagtc
     1261 cggcggcgtg cgggtagaaa gtgaagggcg acgttttcgt ctcgccggac aggccgcaat
     1321 tgaaaagttt cacgtccagc ccatagagag cgacgttggt gctgagcagg tcgaaggtcg
     1381 ggggaatcgg ctcgaaggcg tagacgacag gttttttgca ctgctggtga acgaagagag
     1441 taaacaggcc gatatttgct cccacgtcga aaatgcagtc gccgtcgttc agggtgatgc
     1501 cctgtttcag gtagacctta tcttcaaaca cttcgcggta aataatgttg gtttggaggc
     1561 cgctgtggtg tgcgacgacc atcccgttcg gcaacctgta gccgcgacgg tctgcgagga
     1621 tgcgctcctg atcaatcggt gttagaggct gttcgtgatc ctgctgccgt gcctgtgcgg
     1681 ggacgagata agcgaccaga cgtttatcac ccggcacgtc ctcgcgcgcg acgacgaccg
     1741 cctcgcgcac gcccgcatgc tccacaatgg ctgtttcgat ctctcccagt tcgatgcgat
     1801 ggccgcgcac cttcacctga tcgtcgatgc gcccgatgaa ctcgatctcg ccgccgggca
     1861 ggtagcgtgc gaggtcgcca gtccggtaga gacgcgcgcc gccttccttg ccgaaggggt
     1921 cggggatgaa tctttcggcg ctcaaatccg gcctcttcaa atacccgcgc accactcccg
     1981 cgccgccgat gaacaactcg ccgggcacat tgaccgggac gggttgcagg tggcggtcca
     2041 gaatatagat ctgtgtattg gcaatcgggc gaccgatagg cacgctgccg ccgtcttttg
     2101 ccagaagttg cgtcgcagac cagatggtcg tctccgtcgg cccgtacatg ttatgtatct
     2161 cagccgccaa ctcctcgctc agatgtgcgg cgagcgcggc ggggagggct tcgccgccga
     2221 gcaacagttg acgcacggag cggagagcat cgagcgcacc cggttgagtc gccagcatgc
     2281 tcgccatcga aggggtgcat tgcaggtgag aaacgctgtg cctttgcagt tgcgccggaa
     2341 gcgaatagtc ttcctccgcc gcatcgtcct tccggttgct ctcttctttg accgcgctca
     2401 ggtagttgag actcgacatc accgagtcga aatcgacgcc gaagtcgacg aggcaagcca
     2461 cctcgtcaac gcctatcgcc ttgagccgct cgacgatttg caggcagctt tccggcgtcc
     2521 cgaaaagccc gctcgtctcg aagtaacggt cgaaggcgcg cgcgagcaac gcctgcatgt
     2581 cgtcctctgt gaagtctttc ccttcgatat cctgccccag actgcgcgcc agatttttga
     2641 tcagtccaaa agaactgcgc aggtaattga tgaagggctg gcgcaccttt tcgcggacgg
     2701 aggcggcgtc tgctccgata aatgtatgaa gcatgagcga gacgtgacct tcgccctgcc
     2761 gggga
//
LOCUS       DASWNS010000070         7101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_470627, whole genome shotgun sequence.
ACCESSION   DASWNS010000070 DASWNS010000000
VERSION     DASWNS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470627"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VGB76_02410"
     CDS             complement(<1..183)
                     /locus_tag="VGB76_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097782.1"
                     /translation="MTKSVSAKEKDVAHESQTNPERAGDPVRSVREEPAGTISVKASR
                     EQEIDPRISYPSYKSLD"
     gene            complement(439..1194)
                     /locus_tag="VGB76_02415"
     CDS             complement(439..1194)
                     /locus_tag="VGB76_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0097783.1"
                     /translation="MRYLLFSLLLLGGSSVSLAHAGVREQRSGLLTRTVKVGADSYDY
                     QVYVPDKVRGQAKLPVILFLHGIGQRGEGGFIPAQGGANVMLKGYLEQIPAIVLLPHC
                     RKGRYWHDAEMERMVLESLRQTVSEFDADESRLYLTGVSMGGFGAWHIASKHPGRFAA
                     LVPICGGSTLIAADRYTPVARGVGKMPVWVFHGADDPIVPVSESRGMVAALKATGGDV
                     RYNEYAGVGHNVWLNVLGEAELLPWILAQRLRR"
     gene            complement(1218..2006)
                     /locus_tag="VGB76_02420"
     CDS             complement(1218..2006)
                     /locus_tag="VGB76_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEY0097784.1"
                     /translation="MTKDKHLEQQIETVARWINESRHLVAFTGAGISTDSGIPDFRGP
                     DGVWTRRDAGLPAPRWRVSPEEVRPNASHAALVELQRLGKLRFLITQNTDNLHRRSGI
                     DPEILAELHGNGHLMRCLACDRLYERRALGWDARRWGTAYRTQKPIAGQPACPDCAGR
                     LISSVVNFGDPLPEKELMLSAEHARRCDLMLVLGSSLVVNPAASLVGLALRNGARAVL
                     VNQGETPYDEAVDLLVRSPIGDVIPPAVERVRQHKSADTSLSKD"
     gene            2142..2843
                     /locus_tag="VGB76_02425"
     CDS             2142..2843
                     /locus_tag="VGB76_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097785.1"
                     /translation="MEEKTKHSLDKGAASEGRLLARPSEVKEASPVGLHKLNLDRQRD
                     GLLFVPRNYRVERPAAFALMLHGAGGNAQHGISLLQRFADESGIILLAPDSRRQTWDV
                     IFDRYGTDVSYIDRALAQTFSRYAVDPKRIAVGGFSDGASYALSLGVTNGELFTHVIA
                     FSPGFMAPGGQQGAPRLFISHGTRDQVLPIQRCSRRLVPQLQGAGYDVLYREFDGAHT
                     VPPEIAREAHEWFIK"
     gene            3130..4167
                     /locus_tag="VGB76_02430"
     CDS             3130..4167
                     /locus_tag="VGB76_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0097786.1"
                     /translation="MSLQTQRERTALAFGAMLRFASVLAILFTLTSAAFAQESSRSIK
                     VERVNFPVTLSDGKTYTVAGYLYYQGSYRNRTLLLALHGANYNHNYWDVPRINGHDYS
                     FARYMAAQKYAVLAIDQLGTGASDKPDGDLLTLGETASATHQIISGLRAGAGDIRHPF
                     ERVVLVGHSLGSINALYEQATYADADAVIITGMGHVAHKLPIPDPVIEEMLKSKYFPF
                     PDNLRAELFYYAPGADPDVIAYDRDHLSELLPRAQLVTGVFAAFNPEANRVGAVTGPV
                     LVQLGEHDALFPASLADGEAAFYQSASSVTVQAIPGVGHDVNTHYGNHASWRLMEEWL
                     RSAGLHKPDAN"
     gene            complement(4430..4864)
                     /locus_tag="VGB76_02435"
     CDS             complement(4430..4864)
                     /locus_tag="VGB76_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0097787.1"
                     /translation="MTSAPHRATSQPERVTNYRILVVEDAPDTRELLCLLFESEGYEV
                     LTAGDGLSAVEIALRELPDAIVMDMSLPRLDGYNAAKLIRREPALAGVPLIACTAFNR
                     WEWRGKAIAAGFDAFLTKPIDIRRLSATMSSLLSREADRPTR"
     gene            complement(4861..5790)
                     /locus_tag="VGB76_02440"
     CDS             complement(4861..5790)
                     /locus_tag="VGB76_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0097788.1"
                     /translation="MLPRENLQASTPDVECGTLVRTPLFYSSILGNARQLIVYLPPGY
                     SADENRRYPVLYMQDGQNLFDGTTSYVPGQHWHLNETADNLIREGAIQPLIMVGVYHA
                     GERRIDEYTPTADPNFKKGGRAARYGRMLAEELKPYIDGAYRTKPDREHTGIGGSSLG
                     GLLSLYLGLGRHGDIFGRVLAMSPSLWWDKCWLLRHLDELTRGQKAKVWIDAGTAEGS
                     NTECNAETLAEALAGRGFTRDAEVKFMRVEGARHSEQDWAHRVHHGLRFTFAADTKEE
                     GNLLPNPNRSHGTAQTQHQLRPVGGVLTSPSTL"
     gene            complement(6210..6596)
                     /locus_tag="VGB76_02445"
     CDS             complement(6210..6596)
                     /locus_tag="VGB76_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0097789.1"
                     /translation="MDRGGLTVLVVDDYADTREFIRTLLEMKGCRVFEAANGREAVNI
                     AANTVLSFILMDLEMPVLNGFEATREILAHQKTVDVPIIAFSANCSQERQQRALDVGC
                     RECIPKPVDFSRIDDLINQYSSNAMD"
BASE COUNT         1399 a         2165 c         2085 g         1452 t
ORIGIN      
        1 gtccaacgat ttatagctcg ggtaggaaat gcgcggatcg atctcttgct ccctgctcgc
       61 tttcacggaa attgtcccgg caggctcctc tctaaccgat ctcaccgggt cgcccgcgcg
      121 ctccggattc gtttgtgatt catgcgccac gtccttctct ttggcagata cacttttcgt
      181 cattgatgga aagcaccctc gctatgttga tagagcggac gaattatagc aggcaagtgc
      241 gattcttcgt ctattccggt gccgaatatt atcactcatc aggagaggcg ctcttggtac
      301 gactacgtac acaccacctt cgattcatct accagacgtt aaactaaatc ctccgttttt
      361 gtctgcggca actcatccgg cgggcgtttt gtctccccgc acggcagaca cgatccgccg
      421 cgccgtgcgc aggcaagctt aacggcgtag acgctgcgcg agtatccagg gcaacagttc
      481 ggcctcgccg agcacgttga gccagacgtt gtggccgacg cccgcgtact cgttatagcg
      541 cacgtcgccg cccgtcgcct tcaaagcggc gaccatccca cgcgattcac tgaccggcac
      601 gattggatcg tccgcgccgt gaaagaccca cacgggcatc ttgcctacgc cacgcgcgac
      661 gggagtgtag cggtcggctg cgatgagcgt gctgccgccg cagatcggca ccagcgcggc
      721 gaacctgccc ggatgtttcg aggctatatg ccatgccccg aaccctccca tcgagactcc
      781 tgtgaggtag aggcggcttt cgtcagcatc aaactccgag acggtctggc gcagagactc
      841 caacaccatt cgctccatct ccgcgtcgtg ccagtagcga cccttgcggc agtgcggcag
      901 caagacgatg gccggaatct gctccaggta accctttaac atcacgtttg cgccgccctg
      961 agccgggatg aagccgccct cgccgcgctg gccgatgccg tgtagaaaaa ggatcacggg
     1021 gagctttgcc tgcccgcgaa ctttgtccgg gacgtaaacc tgataatcat aagagtccgc
     1081 gccgactttg accgtgcggg tcaagaggcc gcttctctgc tctcgcactc cggcgtgtgc
     1141 gagggagacg gaacttccac cgagcagtag aagcgagaac aatagataac gcatgtaaaa
     1201 agtttaacct tcctttttta gtccttgctt agtgatgtat ccgcgctttt gtgttgcctg
     1261 acacgctcga ccgcaggcgg gatcacgtcg ccgatgggcg aacgcaccag caagtccacc
     1321 gcttcgtcgt aaggcgtctc gccctgattg accagcactg cgcgcgcccc gttcctcaag
     1381 gccagcccga cgagcgatgc cgccgggttc acgaccaacg atgaacccag tacgagcatc
     1441 agatcgcagc ggcgtgcgtg ttctgccgag agcatcaact ctttctccgg cagcgggtcg
     1501 ccgaagttca ccacggaaga gataagacgc ccggcgcaat cggggcaggc gggttgtccc
     1561 gcaataggtt tttgcgtgcg gtaggcggtt ccccaacgtc gcgcgtccca cccgagcgcg
     1621 cgtcgctcat agagacggtc gcaggccaga caacgcatga ggtgtccgtt gccgtgcaac
     1681 tctgccagaa tctccgggtc tatcccggaa cgcctgtgga ggttgtccgt gttctgcgtg
     1741 atgaggaagc ggagtttgcc cagacgttgg agttcgacca gagccgcgtg cgaggcattg
     1801 ggacgaacct cttcgggcga gacgcgccag cgtggcgcgg gcaacccggc atcgcggcgc
     1861 gtccacacgc cgtcggggcc gcgaaagtca gggatgccgg aatcggtgct gatgcccgcg
     1921 ccggtgaagg caacgaggtg tcggctctcg ttgatccaac gggcgacggt ttcgatctgt
     1981 tgttcgaggt gcttatcctt agtcatgcta ttttgatgaa taagaggcag atgaagtttg
     2041 caggacgggt aacgcggcga cgctttttag gcttcgggct agcgccactg gcggcgattt
     2101 gtggtgcgtg cgtcgccgag cgggcgagag gggatttatc tatggaagag aagaccaagc
     2161 atagtctcga caagggagcg gcctccgagg gacgcttgct ggcacgcccc tcggaggtca
     2221 aagaagcgtc tcccgtgggg cttcacaagc tgaatctcga ccgccagcgc gacgggctgc
     2281 tctttgtgcc tcgaaactac cgcgtagaac gtccggctgc gttcgcgctc atgctgcacg
     2341 gcgcgggcgg caatgcgcag cacggcatct ccctgctcca gcgttttgcc gacgagagcg
     2401 ggataattct ccttgccccg gattcacgcc gtcagacgtg ggacgtgatc tttgatcgct
     2461 acggcacaga tgtcagctac attgatcgcg cgctggcgca gaccttcagc cgctacgcgg
     2521 tagacccgaa gcggatcgcg gtcggcgggt tttccgacgg cgcatcctac gcgctttcgc
     2581 tcggagttac gaacggggag ctattcacgc acgtcatcgc gttctcgccg ggcttcatgg
     2641 cgccgggcgg gcagcaaggt gcgccgcgcc tctttatctc gcacggcacg cgggatcagg
     2701 tgctccccat ccagcgttgc agccgccgcc tggtgccgca attgcagggc gcgggttacg
     2761 acgtgctgta ccgcgaattc gacggggcgc acacagtccc gccggagatc gcacgcgagg
     2821 cgcacgagtg gttcatcaag tagtcgcctt gcttcgatca actcgcgtgg aggaacaacc
     2881 tgttcttaac actaatttcg tcctgtgtgc gttcgagacg ccgcctgcct ttaccttcgg
     2941 atgacgaagc cccgcacgtc gaatttcacg ccgcgtaaat tttcctgatc cctttgtcct
     3001 gtagttttcg cgttaccttc ttgagacaca aaacgcgcct tttcacaggc cgcacgccgc
     3061 catccgcccg gcattacttt gccgacgcgc cttacaggac aaaagaataa ggagacaaaa
     3121 gaaaagaata tgtcattgca aactcaacgc gaacggacag ccctggcctt cggggcgatg
     3181 ctcagatttg cgtccgttct cgccatcctc ttcacgctga ccagcgcggc cttcgcacaa
     3241 gagtcgtccc gcagcatcaa ggttgaacgc gtgaacttcc cggtcacgct ctcggacggc
     3301 aagacctaca cagtcgcagg ctacctctac tatcaaggca gctaccgcaa ccgcactctt
     3361 ctgctcgcac tgcacggcgc aaactacaac cacaattatt gggacgtgcc ccggatcaac
     3421 ggccacgact actctttcgc ccgctacatg gccgcgcaga agtatgccgt gctcgccatc
     3481 gaccaactcg gcacgggcgc gagcgacaaa cccgacggcg acctcctgac gctcggcgaa
     3541 actgccagcg cgacccacca gatcatctct ggtttgcgcg cgggcgcggg cgacattcgc
     3601 caccccttcg agcgcgtcgt gctggtcggc cactcgctcg gcagtatcaa cgccctctac
     3661 gagcaggcga cctacgcgga cgccgatgcc gtcatcatca cggggatggg gcacgtcgcg
     3721 cacaagctgc cgatacccga cccggttatc gaggagatgt tgaagagcaa atactttccg
     3781 ttccccgaca acctgcgtgc ggaactcttc tactacgcgc ccggcgcaga ccccgacgtg
     3841 atcgcctacg accgcgatca cctgtccgaa ctcctgccac gcgcgcaact cgtcacgggc
     3901 gtcttcgcgg cgttcaaccc ggaagccaac cgcgtcggcg cggtcacagg acccgtgctc
     3961 gtccagcttg gagagcacga cgcgctcttc cccgcttcgc tggcggacgg cgaggcggcc
     4021 ttctaccaga gcgcgtcgag cgtcaccgtg caggcgattc ccggcgtcgg acacgacgtg
     4081 aatacgcact acggcaacca tgcgagttgg cggctcatgg aggagtggct caggtcagcg
     4141 gggcttcaca agccggacgc caattgatct ccccgcacag ttctcgaaaa ggagaggtca
     4201 acgctctctt gtgacagcgt tgacctcccg ctctacggcg cattgtctgg ctggttgtgt
     4261 tgcgggaatt agacggaaat acaacagcgg aagcacagcg acgaaagcgc agcagacgca
     4321 aattcaattc gcgaggtacc gtcggaaccg gagaacccat cgcccggctc ctacttgcgc
     4381 gcaccgcttg atataaaccc cattcgagaa ttctctcgca cgcccacgcc taacgtgtag
     4441 gacgatccgc ctcgcgcgac agaagcgacg acatggtcgc cgacaaacgc ctgatgtcga
     4501 tgggcttggt caggaaggcg tcgaacccgg cggcgatagc tttgccgcgc cattcccaac
     4561 ggttgaaggc cgtgcaggcg atgagcggca cgcccgcgag ggcaggctcc cggcgtatga
     4621 gtttcgcggc gttgtagccg tctaagcggg gcaggctcat gtccatgaca atcgcgtcgg
     4681 gcagttctct gagcgcgatc tcgaccgcag ataacccgtc acccgccgtc aagacctcgt
     4741 aaccctcgga ctcgaacagc aggcacagga gttcgcgcgt gtccggcgca tcttccacca
     4801 ccagaatgcg gtaattcgtg acacgttcgg gctggctcgt tgccctgtgc ggcgcgctgg
     4861 tcataatgtc gaggggctgg tcagcacgcc gccgaccgga cgcagttgat gttgagtttg
     4921 cgcggttcca tgtgagcggt ttgggttcgg cagcaaattc ccctcctctt tggtatcggc
     4981 ggcgaatgtg aagcgtagcc cgtggtgtac gcggtgcgcc cagtcctgct cggaatgacg
     5041 cgcgccctca acccgcatga actttacctc cgcgtcgcgc gtaaatccgc gcccggccag
     5101 agcctctgca agcgtctcgg cgttgcattc ggtgttcgag ccttccgccg tcccggcgtc
     5161 gatccagact ttagcctttt gtccacgcgt caattcgtcg aggtgacgta gcaaccagca
     5221 cttgtcccac cacaacgaag gcgacatggc gagaacgcgc ccgaagatgt cgccatgtcg
     5281 cccaagtccc agatacaggc ttaacagccc tccgagcgac gagcctccga tgcctgtgtg
     5341 ctcgcggtcg ggcttcgtcc tgtacgcgcc gtcgatgtaa ggcttcaact cctctgcgag
     5401 catccgcccg tagcgcgctg cccggccacc cttcttgaag ttgggatcgg ctgtcggggt
     5461 gtactcgtca atgcggcgtt cgcccgcgtg gtagacgccg accataatca agggctggat
     5521 ggcgccttcg cggatcaggt tgtcggctgt ctcgttcagg tgccagtgct gccccggcac
     5581 gtatgaagtt gtgccgtcga agagattctg cccgtcctgc atgtagagga cggggtatct
     5641 gcgattttcg tccgcgctgt agccgggcgg gagatagacg atgagttggc gtgcgttccc
     5701 gaggatggac gagtaaaaga gcggtgtgcg aacgagcgtg ccgcattcta cgtccggcgt
     5761 tgacgcctga aggttttcgc gaggtaacat ttttggctac tgtccccaat caaaacaata
     5821 atggtcgcgc cgacctcgca gaggaatttt tgtactattt cccatcggcc aatcggtcgc
     5881 gtgggaaggc agagcgattc atgtgccact gctgaatgcg ggcgtttggt tcgttcgctt
     5941 tgaagggttg gaggggttgg agggtaagat aaggagagtg cttgatccgt caagcggcag
     6001 acgacctgag ggattttccg gacggcacag ggcttggatg cagcttggat gcaagcctcc
     6061 ggttcatctg cgaaggtatc tttacaggcg ggcaactctc cggctgaaag ataatgcggc
     6121 atactttaga caggcgcacg ggagccgtta aagacctgag gccaacggga cgagcggtaa
     6181 atgtgtggaa cggcgtaacg cgtccggcct taatccattg cgtttgagga atactggttg
     6241 attagatcgt caatacgaga aaagtccacg ggtttaggga tacattctct gcaaccgaca
     6301 tccagtgcgc gctgttgtcg ttcctggcta cagttggcgg agaaggcgat gatgggaaca
     6361 tccacggtct tttgatgtgc gagaatttcg cgcgtagctt cgaagccgtt gagcaccggc
     6421 atctccaagt ccatcagaat gaagctcagt acggtattcg ccgcgatgtt taccgcctct
     6481 cgaccgttcg cggcttcaaa cacccggcaa cctttcatct ccaacagtgt cctgatgaat
     6541 tctcgcgtgt ccgcgtaatc atcaacgacg agcacggtca gaccccctcg atccatgttc
     6601 aagtcctctc taagtgcagg gatatggaaa ctctttaata gcacatctca cttacccgga
     6661 cgaagctgac aggccgcctg cccggcaggc agctcgctac ttaaagagcg cggcaaaaaa
     6721 attgtgccgc attactacag atgttgggag agttttgcta gtagtaaggg cagggttctt
     6781 tagctgtgct cttattacat ccccgtcgaa ggcggacgtt gccagcactc taaactcgcc
     6841 gtgcccctgt gtatataaaa gagtagtgct gcaaaaaata tcttgtctcc gtttagtcgc
     6901 cttgagaagc ctgtggttgc cttcatagct tgagtgagcg gaaatttttg acaacactac
     6961 cctcgacagt ttccacacct tctacgccgc aagcgtgagc gcgccgtcac acgtcacggc
     7021 gaagccgacg gagacgacgc gccgacgttg aaaatcattg acgagcagca ttgctgcctg
     7081 agcgcgtctt tgtctcggcg g
//
LOCUS       DASWNS010000071         4239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_106865, whole genome shotgun sequence.
ACCESSION   DASWNS010000071 DASWNS010000000
VERSION     DASWNS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106865"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..694)
                     /locus_tag="VGB76_02450"
     CDS             complement(326..694)
                     /locus_tag="VGB76_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097790.1"
                     /translation="MKQTELFAEKKAEAAHDIDRVVLYAFADFQLRGKTLAERELPLD
                     RLRGALRRAAEVFGISELNDETAAAAIQTLGGRVRRVPPYVAKHPFRITVPAELAERA
                     RLLYQNQDAPVDASTGDSGT"
     gene            complement(764..1366)
                     /locus_tag="VGB76_02455"
     CDS             complement(764..1366)
                     /locus_tag="VGB76_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097791.1"
                     /translation="MWRGAEKLWRCLLLAMLVCVASAGTAAAQRANTAAADEAEFGPV
                     VRTYLGYLRAEQEVVDDRASRREIDQTYFRRNSNRIRALRQMAVRIARETQNDFLPEL
                     YAVTRDELGTLFDPEPQPSEFRVGEIVNNTFRYLGTVRVAEPFYLFARLDIYEQAELS
                     QKGQAQPAPDATASAPDATTANAPRGKNTATTRPRRVNTP"
     gene            1599..2951
                     /gene="coaBC"
                     /locus_tag="VGB76_02460"
     CDS             1599..2951
                     /gene="coaBC"
                     /locus_tag="VGB76_02460"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY0097792.1"
                     /translation="MSTVSTDTPATHAHAGRRRVALGISGGIAAYKAAEILRGLQRAG
                     CEVRVAMTEHATKMIAPLTFRALSGAHVVVDDYAPDNPDPIAHITFSQTVELFLIAPA
                     TANIIAKFANGIADDFLTSTYLASNAPVVIAPAMNTTMWQHPATVRNLRRLREDGVYI
                     IEPDAGEMACGTFGPGRLSEPEKIVAAALKILEQHAAGASSASKPSLSGSTGAEDEGA
                     QVDDRRVRDLAGEKFLITAGATREEIDPVRFLSNRSSGRMGFALAEAAAARGAEVSIV
                     AGATSAGAPARARIIRATSADEMHAAVRAEIKSATVFIGAAAVSDYRPARRAAQKIKK
                     SGAALTLTLEPTPDILAAVAAERHEELLVIGFAAESENVVANAREKLSRKKLDAIVAN
                     DITRDGAGFDAETNIVTIITRGGDAHVELPLMSKLETAHRILDEVVRLRRARVAQAAG
                     "
     gene            2990..4039
                     /locus_tag="VGB76_02465"
     CDS             2990..4039
                     /locus_tag="VGB76_02465"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0097793.1"
                     /translation="MADEASRAELLSLTEEVGEALSYLRELGVDYLESAGTIEAAATT
                     QAFDIRSSSARGADAPAPDAQRFAALKRNAEAGARVAQPPAETHAAGTQRPATPPPYA
                     RTEERTAPDILYQSARTTSTERAEMPKRKPIRTPDPVPPPPQETLFGEIKPKDELSLP
                     REGETFADIHRDVGECLRCPLHQSRTRIVHSEGNAKARLMFVGEAPGADEDASGRPFV
                     GRAGQLLNKIIEAIGMKREDIFIGNVNRCRPPQNRTPTHEEAVTCKPFLLREIAILRP
                     DVIVVLGNTAMKNLLDTKEGITKLRGQFMDYKGIKVMPTFHPAYLLRDPSKKRETWED
                     MKKVREYLNKTKTDK"
BASE COUNT          875 a         1272 c         1324 g          768 t
ORIGIN      
        1 gccgtgtaat cgtgtgcccg ccatgtcggt tattgcgcat cgggcacgac ttctatcatg
       61 cgatagaagt gtgtagcaac tgaaagtctt gcaccgaagg tgcatagttt tgcactagcg
      121 tgtctgtgac aataaactca aaacaggaaa cagaaattat agccgccgag aagtgcgtgc
      181 gatttagagc gcgtttgaaa accgcttgat gccttccccg atgtctgtgc gtgagtatag
      241 ttttcccgca aaggcgcaag ggacggcaag gaagacgcag agcgtcagag tagttattcg
      301 tagttttgaa ataggctccg gggcttcaag tgccggaatc gccggtcgag gcatcaacgg
      361 gggcgtcctg attttgataa agcaggcgcg cacgttcggc tagctcggca gggacggtga
      421 tgcgaaaggg gtgcttggcg acgtaaggcg gcacgcggcg cacacgcccg ccgagcgttt
      481 gaatcgctgc cgcggcagtc tcatcattca actcggagat gccgaaaact tctgcggcgc
      541 gacgcaaggc tccgcgcagc cggtcaagcg gcagttcacg ctcggcgagc gtcttgccgc
      601 gcagttgaaa gtctgcgaag gcgtagagca cgacgcggtc tatatcgtgt gcggcttcag
      661 ctttcttctc ggcaaaaagt tccgtctgct tcatcgtgaa ttatggcgcg tgcgtagcgg
      721 tacacaaatt ccgcgcgccg ctctttctcc gcacgcggct ggctcaaggt gtgttaacgc
      781 ggcgcgggcg cgttgtcgct gtgtttttgc cgcgcggggc gttggccgtc gtcgcatcgg
      841 gagcagaggc ggtcgcatcg ggggcgggct gcgcctgtcc cttctgcgac agttcggcct
      901 gttcatagat gtcgagccgc gcgaaaagat agaacggctc ggcgacgcgc accgtgccga
      961 gataacgaaa agtgttgttg actatttcgc cgacgcgaaa ctcggagggt tgcggttccg
     1021 ggtcgaacag agttccgagt tcatcgcgcg tgacggcata gagttcgggc aagaaatcgt
     1081 tctgcgtctc gcgcgcgatc cgtacggcca tctgcctgag cgcgcggatg cggttcgagt
     1141 tgcgacggaa ataggtctga tcgatctcgc gacggctcgc gcggtcgtca accacctcct
     1201 gctcggcgcg caggtagccg agataagttc gcacgacggg accaaactcc gcttcatcgg
     1261 cggcagccgt gttcgcgcgt tgcgccgccg ccgtgcccgc cgaagcgacg cacacgagca
     1321 ttgccagcaa caggcatcgc cacaatttct ctgcccctct ccacatgttt ttcacagacc
     1381 ccggcacagc gaaagagctg cgctgtgttt aactttgcgt ttaactttgc gtctgacttg
     1441 aagttgattt caagcccctc gaaagtttac cacttccggc gcgtcaaagg ttagaggtcg
     1501 caagtctgcc ggacgggcga acgcccggaa agctgcgaag tttcagtttg cgcccgtcgc
     1561 gccgcgatgc tacatttttg ccgtaccttt caacctttat gtcaactgtc tcgacagata
     1621 caccggccac gcacgcgcac gcgggcagaa ggcgcgtcgc gctcggcatc tcgggcggca
     1681 tcgccgctta caaggccgca gagattctgc gcggactgca acgcgccgga tgcgaggtgc
     1741 gcgtggcgat gacggaacac gcgacgaaga tgatcgcgcc gctcaccttc cgcgctctct
     1801 ccggcgcgca cgtcgtcgtt gatgattacg cgccggacaa tccagacccc atcgcgcaca
     1861 tcaccttctc tcaaaccgtc gaactctttc tgattgcgcc cgcaacggcc aacatcatcg
     1921 ccaaattcgc gaacggcatc gccgacgatt tcctgacttc aacttatctg gcctcgaatg
     1981 cgcccgtcgt catcgccccc gcgatgaaca cgacgatgtg gcagcatccc gcgaccgtgc
     2041 gcaatctccg acgcttgcgc gaagacggcg tgtacatcat cgaacccgat gcgggcgaga
     2101 tggcctgcgg cactttcggg ccggggcgtc tcagcgaacc cgaaaagatc gtcgcggcgg
     2161 ccttgaagat tcttgagcaa cacgcggcag gcgcgtcgag cgcgtcaaag ccttctctgt
     2221 ccggttcgac cggggcagag gatgagggcg cacaggttga cgacagacgc gtgcgcgatc
     2281 tggcgggcga aaaatttctg atcacggctg gcgcgacgcg cgaagagatc gatccggttc
     2341 gttttctctc gaaccgttcg tctggacgca tgggtttcgc gctggccgaa gcggcggcgg
     2401 cgcgcggggc ggaagtctca attgtggccg gggcgacgag tgccggagcg cccgcgcgtg
     2461 cacgtatcat tcgcgcgacg agtgcagacg agatgcacgc ggcggtgcgc gccgagatca
     2521 aaagcgcgac cgtcttcatc ggggcggcgg cagtctccga ttatcgtcct gcgcggcgcg
     2581 ccgcgcagaa gatcaaaaaa tccggcgcag ccctgacgct cacgctcgaa ccgacgcccg
     2641 atattctcgc cgccgtcgca gccgaacgtc acgaggaact gctcgttata ggctttgccg
     2701 ccgagagcga aaacgttgtc gccaatgcgc gtgaaaaact gtcgcgaaaa aaactcgacg
     2761 ccatcgtcgc caacgacatc acgcgcgacg gcgcaggttt cgacgcagag acgaacatcg
     2821 ttaccatcat cacacgcgga ggcgacgcgc acgtcgagct tccgctcatg tcaaaactcg
     2881 aaacggcgca tcgtattctg gatgaagtcg tgcgcttgcg gcgggcgcgt gtggcgcagg
     2941 cggcgggata gttgaaggag tagcggcgaa agagtttgag aagattgcta tggcagatga
     3001 agcgagtcgg gcggagttgt tgtcgctgac ggaagaggtc ggcgaggctc tgtcgtacct
     3061 gcgcgaactt ggcgtggatt atttggagtc tgcgggtaca atcgaggccg cagcgacgac
     3121 gcaggcgttt gacatcagaa gcagcagcgc gcgtggggcg gacgcgcccg cgccggacgc
     3181 gcagcgtttc gccgcgctga aacgaaacgc agaggcgggt gcgcgcgtgg cgcagccccc
     3241 ggcggaaact cacgcggcgg gcactcaacg cccggccacg cccccgccgt acgcgcgcac
     3301 ggaagagcgc acagcccccg acattttgta ccagtcagcc cggacaactt cgacggagag
     3361 agccgagatg ccgaaacgaa aacccatccg cacgcccgac cccgtcccgc cgccgccgca
     3421 ggagacgctc tttggcgaga tcaagccgaa ggacgaactg agcctgccgc gcgagggcga
     3481 aaccttcgca gacattcaca gagacgtggg cgagtgcctg cgctgcccgc tgcaccagag
     3541 ccgcacgagg attgtgcata gcgagggcaa cgctaaagcg cgcctgatgt ttgtcggcga
     3601 agcgccgggc gcggacgaag acgcgagcgg gcgtcccttc gtcggtcgcg ccgggcaact
     3661 cctgaacaag atcatcgaag ccatcggcat gaagcgcgaa gacatcttca tcggcaacgt
     3721 caaccgctgc cgcccgccgc agaaccgcac gccgacgcat gaagaggccg tcacctgcaa
     3781 gccgtttctg ttgcgcgaga tcgccatcct gcgcccggac gtgatcgtcg tcttaggcaa
     3841 cacggcgatg aaaaacctgc tcgacacgaa ggaaggcatc acgaaactgc gcgggcagtt
     3901 catggactac aaaggcatca aggtgatgcc gacgtttcac ccggcctacc tgctccgcga
     3961 cccttccaag aaacgcgaga cgtgggaaga catgaagaag gtgcgcgagt atttgaacaa
     4021 gacgaaaacg gacaaatgag tctcgcgccc cgactcaacc cttcaagtct ctgcgcccgt
     4081 ccgtgaactt attccttaac aagaataagt ctcacgcaca ggcgcaaaga gagcgagagt
     4141 ttgagtttgt gaagttcctg tgggtgagat cagtcttata gaatcatggc cgagctttca
     4201 cttttcgacg atgcgccgcc cggctccgac gagacgctc
//
LOCUS       DASWNS010000072         4749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_54652, whole genome shotgun sequence.
ACCESSION   DASWNS010000072 DASWNS010000000
VERSION     DASWNS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54652"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1354
                     /locus_tag="VGB76_02470"
     CDS             71..1354
                     /locus_tag="VGB76_02470"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0097794.1"
                     /translation="MPALVEPTAPRETRSSEMFRSLAHRNFRLFWAGAFLSNIGTWMQ
                     TVAQGWLVLKLTNSAFWLGVDGFAATSPGLVLTLLGGVYADRIDRKRLLIYTQAGAGL
                     TALVLAILIATGVVRDESHVWIILVASFITGCCFSLASPSYQAITYDLVGREDLTNAI
                     ALNSTQFQLSRVVGPALAGVGFQLFGLAGCFFVNGLSFVAIIIALSLVRFESPGGTTV
                     VAQPAGERNSMWHDLKNGFRYVWRRPRVFLLLLISAVASLFGAPYMTLMPFFTRNVLG
                     LGETGLSVMMGVAGAGSFLSALMLTFLGNVRRKGLYVLGGSAAAGACIVGFALSRSVP
                     LSLLMLFGIGFSIVCSIAVTNMLLQELVTDEMRGRVMSMFMLSFIGAWPIGSFLAGVS
                     ARRFGAPHTLAVGGLVIVLFNLYVALRSPRLRAMN"
     gene            1537..3873
                     /locus_tag="VGB76_02475"
     CDS             1537..3873
                     /locus_tag="VGB76_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HEY0097795.1"
                     /translation="MPSRRFAASLFFNFLIFFTFAAAATVYAQGVIVPRPCETCPRPP
                     RPLPPLPPALPIKSIKIETKINAQVATTHVEQIFRNDSDATLEGTYFFPIPETASISE
                     FAIWDGERRLVGEVRSREEARRIYDQIVRKQRDPGLLEYAGKDLFQASIFPIPPHADK
                     KLELTYTQVLRAQSGTVSYRYPLGTNHNLVSIGRVSGALEIEGNKPLRNIYSPSHAVD
                     VRPSQGGQRARVSFETTGAGREPQDFQLFYTLSSEDFGMSLLTHREPGKDGYFLLMIS
                     PKDNWAESEYTTKDIVFVIDTSGSMAEEGKMEKARAAMLFGVKTLRAGDRFNVISFAG
                     EEHLMEAGLIQADERGRARGIEFIQKLQPTGGTNINGAVQAGLKQFDSSDRPKMLIFM
                     TDGLPTVGVTNPPAIIENARTAKTGNTRLFTFGVGYDVNTALLDKLASENGGTADYVE
                     PKEDLEVKVSSFFAKVNYPVLTDLALDMGGVETDFMYPRALPDLFRGAQVTLIGRYRN
                     ANDLRDVRLRLSGRSNRETRSFAYENLRFPVNSEENDFLPRLWATRRVGWLMEQIRSN
                     GEARELRDEVVDLGTRYGIVTPYTSYLALEPGVAGETVDVAGGRARDSSGRVIDGSDN
                     RVALLRRQERRRAAGAPGTGGGAPVAVAPPMLQATPMPTPEAVPPPTTGATAVQQSKQ
                     ARDQQETLIISEDDAATVGRVMRKVADKTFYLREGVWTDADFKADAKLPETALVFGSD
                     AYFDLLKRERKLADFFALGERVVVVYKGRVYRVAAATN"
     gene            4129..>4749
                     /locus_tag="VGB76_02480"
     CDS             4129..>4749
                     /locus_tag="VGB76_02480"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY0097796.1"
                     /translation="MKDAKVVRPYLVQRSAWAFLFPVFCLLVVGCWFAPAGGAQEDGI
                     GWTGTRHGAAGKDLNTVYFTDSKRGWIAGDDGLVFRTEDGGRSWVRQPVGTNEQINDL
                     YFRDKEDGYLIAGSSILITADSGRTWREARRFRPADFGGGVPELYSVRFASKKKGWIV
                     GSVSRREVVTDSLVHFTDDAGATWRPQLVPSRDELIHLDFADEKRGW"
BASE COUNT          860 a         1494 c         1471 g          924 t
ORIGIN      
        1 gcgtgagtct gcgctatttc gacggcggcg gtttgagtca aacgccctcc gaatggtatc
       61 aagttaaatt atgcctgcac tggtcgaacc aaccgcgccg cgagagactc gttcgagcga
      121 gatgtttcgc tcgctcgcgc atcgcaactt tcgtctcttc tgggcgggcg cgttcctgtc
      181 gaacatcggg acgtggatgc agacggtggc gcagggctgg ctggtcctga aactcacaaa
      241 ctccgctttc tggctcggcg tggacggctt tgcggccact tcgcccggcc tcgtcctgac
      301 gctcctcggc ggcgtctatg cagaccgcat agaccgcaag cgcctcttga tctacacgca
      361 ggcgggcgcg gggctgacgg ctctggtgct cgccatcctg atcgctacgg gcgtcgtgcg
      421 cgacgagagc cacgtctgga tcattctcgt cgcctctttc atcacgggct gctgtttctc
      481 tctggcctcg ccctcctatc aggcgatcac gtacgacctc gtggggcgag aggatttgac
      541 taacgccatc gcgctcaact cgacgcagtt tcaactctcg cgcgtcgtcg gccccgcgct
      601 cgccggagtc ggcttccaac tcttcgggct ggcgggttgc ttcttcgtca acggcctctc
      661 gttcgtcgcc atcatcatcg cgctctcgct cgtgcggttc gagtctcccg gcgggacgac
      721 cgtggtggcg caaccggcgg gcgagcgaaa ctcgatgtgg catgatctga agaacggctt
      781 tcgctacgtg tggcgacgtc cgcgcgtctt tttgctgctc ctgatctcgg cggtggcgag
      841 cctgttcggc gcgccttaca tgacgctgat gccgttcttc acgcgcaacg tgctcggcct
      901 cggcgaaacc ggactctccg tgatgatggg cgtggcgggc gcaggctcgt ttctctccgc
      961 cctgatgctc acgttcctcg gcaacgtgcg gcgcaaaggt ctgtacgtgc tcggcggctc
     1021 ggcggcggcg ggcgcatgca tcgtcggctt cgcgctctcg cgcagcgtgc cgctgtcgct
     1081 cctgatgctg ttcggcatcg gcttctccat cgtctgttcc atcgccgtca ccaacatgct
     1141 gttgcaggaa ctcgtgacgg acgaaatgcg cgggcgcgtg atgagcatgt tcatgctctc
     1201 gttcatcggc gcgtggccga ttgggagttt cctcgcgggc gtctccgcgc gtcgcttcgg
     1261 cgcgccccac acgctcgccg tcggcggact cgtcatcgtc ctcttcaacc tctacgtcgc
     1321 gctccgcagc ccgcgcctgc gcgcgatgaa ctgacaacca aaaccgtgaa tcgtgaatcg
     1381 taaatcgtga atagattaaa acccgctttt ctattcacga tttacgatct accgtcttcc
     1441 cgcaagaaag cctcaggcgg tcgttctagg agatgggagc ggcctaaaaa cagtgcccgc
     1501 cgcccgtcaa attggatttt cgaggagaag ccctttatgc ccagccgacg atttgccgcc
     1561 agcctctttt tcaacttcct gatcttcttc acgttcgccg ccgccgctac tgtctacgcg
     1621 cagggcgtca tcgtgccgcg cccctgcgag acgtgcccgc gcccgccgcg ccccttgccg
     1681 ccgctgccgc ccgcgttgcc catcaagtcg atcaagatcg agacgaagat caatgcgcag
     1741 gtggcgacca cgcacgtcga acagattttc cgcaacgatt ccgacgcgac gctcgaaggc
     1801 acctatttct ttcccatccc agagacggct tccatctccg agtttgcgat ctgggacggg
     1861 gagcgtcgcc tcgtcggcga ggtgcgttcg cgcgaggagg cacggcgcat ctacgatcag
     1921 atcgtgcgca agcagcgcga ccccggcctg ctggaatatg ccggtaaaga tctgtttcaa
     1981 gcgagcatct ttcccatccc gccgcacgcc gacaagaagt tggagttgac ctacacgcag
     2041 gtgttgcgcg cacagtcggg cacagtctct tatcgctacc cgctcggcac gaatcacaac
     2101 ctcgtctcaa tcggcagggt gtcgggcgcg cttgagatcg aaggcaacaa gcccctgcgc
     2161 aacatctatt cgccgagcca cgccgtggat gtgcgcccct cgcagggcgg gcagcgggcg
     2221 cgcgtctctt tcgagacgac gggcgcgggg cgcgagccgc aggacttcca actcttctac
     2281 acgctctcca gcgaagactt cgggatgtcg ctcctgacgc accgcgaacc gggcaaggac
     2341 ggctatttcc tcctcatgat ctcgccgaaa gataactggg cggagagcga gtacacgacg
     2401 aaagatatcg tcttcgtgat cgacacttcc ggttcgatgg ccgaggaagg gaagatggag
     2461 aaggcgcgtg cggcgatgct cttcggcgtc aagacgctgc gcgcgggcga ccgtttcaac
     2521 gtcatctcgt tcgcgggcga agagcacttg atggaggccg gactcattca ggcggacgag
     2581 cgcgggcgtg cgcgcggcat agagtttata cagaagttgc agccgacggg cggcacgaac
     2641 atcaacggcg cggtgcaggc cggactcaaa cagttcgact caagcgaccg acccaaaatg
     2701 ctcatcttca tgacggacgg cctgccgacc gtcggcgtga cgaacccccc ggccatcatc
     2761 gaaaacgcgc gcacggcgaa gacgggcaac acgcgcctct tcaccttcgg cgtgggctac
     2821 gatgtcaaca ccgcgctgct cgataaactc gcgagcgaaa acggcggcac ggcggattac
     2881 gtcgagccga aagaggattt ggaagttaag gtttccagct tcttcgccaa ggtcaattat
     2941 ccggtgctca ccgatctcgc gctcgacatg ggcggcgtcg aaaccgactt catgtacccg
     3001 cgcgcgctcc ccgatctctt tcgcggcgcg caggtgacct tgatcggacg ctaccgcaac
     3061 gcgaatgatc tgagagatgt gcgcctcaga ctttcgggca ggagcaaccg tgagactcgc
     3121 agcttcgcct acgaaaatct gcgcttcccg gtcaactcgg aggagaacga ttttctgccg
     3181 cgcctgtggg cgacgcggcg cgtcggctgg ttgatggagc agattcgctc gaacggcgag
     3241 gcgcgagagt tgcgcgatga agtggtcgat ctcggcacgc gctacggcat cgtcacgcct
     3301 tacacgtcat acctcgcgct cgaaccgggc gtggcggggg agacggttga tgtcgcgggc
     3361 ggcagagcca gagattcgtc ggggcgggtg attgatggat cggacaacag ggtggcgttg
     3421 ttgcggaggc aggagcgcag aagggcggcg ggcgcgcccg gcacaggcgg aggtgccccg
     3481 gtggcggtcg cgccgccgat gctgcaagcg acacccatgc cgacccccga agcagtgccg
     3541 ccgccgacga ccggcgcaac cgccgtgcag cagagcaagc aggcgcgcga ccagcaggag
     3601 acattaatca tcagcgaaga cgatgccgcc accgtcgggc gcgtgatgcg caaggttgcg
     3661 gacaagacct tctacctgcg cgaaggcgtc tggacggacg cggacttcaa ggcggacgcg
     3721 aaactgcccg aaaccgcgct cgtgtttggc agcgacgctt acttcgacct cctgaaacgt
     3781 gagcggaaac tggcagactt cttcgccctc ggcgaacgcg tcgtagttgt ctataaaggg
     3841 cgcgtctatc gcgtcgctgc cgcgacgaac tgaaccggaa agaggatagg ggaaggggaa
     3901 aggttaaggt ttagagagcg tgcgacgaac ggcgtgctct tttaaccttt cccccttctc
     3961 cttcctctaa cctttccccc tcccccttct cctttccctt cctcttgccc cttgtctgat
     4021 agcccctgct tcgtgtaaaa cgaagcctac ctcacgcgtc ggaaatatcg tgcaagggcg
     4081 tcgctcgctg tatctcgaaa tcaaacttcg ttcggagaag actcttaaat gaaagacgcg
     4141 aaagttgtgc gcccctatct cgtgcagaga agcgcgtggg cgtttttgtt tccggtgttc
     4201 tgcctgttag tggtcggctg ctggttcgct cccgcaggcg gggcgcagga agatggcatc
     4261 gggtggacgg gcacgcgcca cggggctgcg gggaaggatt tgaacacggt ctatttcaca
     4321 gacagcaagc gcggctggat agcgggcgac gacggactcg tctttcgcac ggaagacggc
     4381 gggcgtagtt gggtacggca acccgtcggg acgaatgaac agatcaacga tctctacttt
     4441 cgcgacaagg aagacggcta cctcatcgcg ggcagcagca tcctgatcac cgccgacagc
     4501 gggcgcacct ggcgagaagc gcgtcgcttc cgccccgcag atttcggcgg cggcgtgccc
     4561 gaactctaca gcgtgcgttt tgcgagcaag aagaaaggct ggatcgtcgg ctcggtcagc
     4621 cgccgcgaag tcgtcaccga cagtctcgtg cacttcacgg acgacgcggg cgcaacgtgg
     4681 cgtccccaac tcgtgccttc gcgcgacgag ttgatccatc tcgacttcgc agatgagaaa
     4741 cgcggctgg
//
LOCUS       DASWNS010000073        18540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_313633, whole genome shotgun sequence.
ACCESSION   DASWNS010000073 DASWNS010000000
VERSION     DASWNS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313633"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VGB76_02485"
     CDS             complement(<1..323)
                     /locus_tag="VGB76_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097797.1"
                     /translation="MKVTKLFVLALVAALAALVYTPVAKLAVDETCTTDVVTEGDIAR
                     RPESTPPTAADDWVLYTRTGTAPTAGAFVNGPATPPAGAGSLQLQTNTGSEKVFIFNY
                     EHEGTR"
     gene            complement(739..2289)
                     /locus_tag="VGB76_02490"
     CDS             complement(739..2289)
                     /locus_tag="VGB76_02490"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEY0097798.1"
                     /translation="MKTTRLPEPDANMRQLLERRASETPAKTFLFDAHDGRSWTYAEF
                     DAAVNRTANLLAAQGVGRGDVVSLLLPNSAEYIIAYFACFKTGALAGPVNSLLKPEEA
                     AYVVGNSKAKALLYHSQFRETVTEIQKEVPRLAAALIFDVETTANANLAATPGESWRE
                     LALSRDDEAIIIYTSGTTGKPKGCLLTHGNLLANARQIVEWMKFTPEDRLLSVMPLFH
                     MNAVMVTTVAAMYAGGSSVVSPRFSASRFWQTISDYGVTSFGSVATMLSMLLATYTEG
                     VPGGLHTEQLRFAMCGSAPVPVEVLRRFEETFRCLVIEGYGLSESTVRATFNPPDARR
                     RPGSCGQSIGCEIKVFDEAEREVATGELGQIVLRGENIFKGYYENEPATREAFRGGWF
                     HTGDVGYRDADGFFYIVDRMSDMIIRGGENIYPREIDELLYKHPAIASAATIGVPDNL
                     YGEEVAAFVVLKEGREASPAEIMEFCRAHLADFKCPKTIRFLPELPKGATGKVLKREL
                     ARKFNRSE"
     gene            2499..3977
                     /locus_tag="VGB76_02495"
     CDS             2499..3977
                     /locus_tag="VGB76_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0097799.1"
                     /translation="MDTYRNFIEGQWILSEAAQTVPNLNPANTDDVLGTVRLSLREEA
                     RRAVEVAHAAFREWRATPAPARGRIIARFARLLEEDTENLAALLTREEGKTLAESRGE
                     IRRAINVAEFCAGEARRMNGETIQSELPANFAYTIKQPHGVVACVTPWNFPVAIPVWK
                     IAPALVAGNTVIFKPATVTPATAVRLVELFAEAGLPKGVLNLVIGSGSEAGDEIVNHP
                     AVRAVSFTGSNPIGLKLYEQCARRGAKVQCEMGGKNPVIVLEDADMNLAVESTAQGAF
                     GSTGQRCTATSRAIVHEKVYDNFIERITARVESLRLGDGSQPDTEVGPLVDGKAFKSV
                     LSYMDIGREDGASLVCGGAIAEGPGLRKGYFVKPTIFVGVTPDMRIAREEIFGPVLSV
                     MRAKDLDEALLIANDSEYGLSSSIFTNDAARIFRFVDEIETGMTHINSPTTGGEAHIP
                     FGGIKATGIGEREQGSTALDFYTELKVVYVDYTGRKREGNLY"
     gene            4071..4616
                     /locus_tag="VGB76_02500"
     CDS             4071..4616
                     /locus_tag="VGB76_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097800.1"
                     /translation="MNAEANVNSACSRCGESLAPALIEQSVNELKRLTAKLEEASAPS
                     FNSFNGFGTTLLDYRASPVGTYEATRWAIALFLPLFPIANYVIQPTGQERSYGRETSK
                     FRIMAQTPLTLTRILRTYALAVAGLLPIIAGSLYSNVVNRTLGGLPAAVAMILSFVWA
                     IYIIFFKLKNEGAAYKSVAAT"
     gene            4660..5964
                     /locus_tag="VGB76_02505"
     CDS             4660..5964
                     /locus_tag="VGB76_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0097801.1"
                     /translation="MSTVEQQVAQSEIVRKHKEFLFPAVATYYQEPVALVRGEGQYVW
                     DDQGTRYLDCFGGVLTVSVGHANPQVNAAIIEQVKTLSHTSTLYANRPQGDLAEKLHQ
                     ITPGRLKKSFFTNSGTEADDTAITAAKIHTGRHEIVVLRHSYSGRSATALSAIGHSTW
                     RPLPAQVAGIVHARAPYCYRCPLKLSYPECGLACAEDIKDVIETTTTGQIAAFMAEPI
                     LGVGGFIVPPAGYFERAAEITRAAGGLFIADEVQTGWGRTGDKWFGIEHWNVEPDIIT
                     SAKGMGNGVPIGMTIATPEVADSFPGLTFSTFGGNPVSMAAALAVIKVIEDENLRTNA
                     ATVGAYFREKLEGLKEKYPVIGDVRGMGLMQGLELVRDRETKEPDPQSLLSVFEETKR
                     RGVLIGKGGLYANVIRTGLMLNSTKDTVDELITALDAGLAAL"
     gene            6039..7487
                     /locus_tag="VGB76_02510"
     CDS             6039..7487
                     /locus_tag="VGB76_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097802.1"
                     /translation="MALTPSDKELLKGIYRKVKDEPLKPGTPEFERFYQPIYEIPGKE
                     DPVDFLQKAIQFSEYESLQLFSGFRGSGKTTELFRLRRRLEQEGYVVLYVDALKYISP
                     SDEIDISDLLIVLAGAFSDTLEEWAAQLQQPVQLAHETYWNRIKNFLTKTSVELNEAT
                     VSLGGETPAGQILGSLKGGLDLKLAIKESPTFRQNLQKLMANRIGELRATVSQFFEDG
                     VKAVKQVWGDEKEVVYIFDSLEQLRGALTNEEGVLKSVQRVFASHLNLLRLPYIHVIY
                     TVPPWLQFVMQGSDIYLLPCIRLWNNDAERTHYEPGWNAVRSLVFKRIGQEGCERLFG
                     CKLDQPFTSADDLIAVCGGHFRDLLRLLGDIVRRADSLPITPDVLNSAVMSLRDDYSP
                     IAIEDALWLAKIGEHRTPMLPDTKSQTISRLTRFLDTHVVLHLTNGVKWYDIHPLVRD
                     EVAKLVKFQQDSQLLKNAQPVGGAPADRSPQP"
     gene            7484..9022
                     /locus_tag="VGB76_02515"
     CDS             7484..9022
                     /locus_tag="VGB76_02515"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0097803.1"
                     /translation="MTKTETSLPTLPAQRSGVAASPEWRSLLTHFELAPDDFAFIVLL
                     VPDRDWAEACRQALERFLISSRKKLLAVHFEDAREFRDELATRLLSLQAGDDIGVVWV
                     SEAVTEAAADYDEWAQAWRVAAGRLNQYRNPLRRQFHVPLIFAGAPWIQVTLREAAPD
                     LWSVRTQVVRIIPPAAEDRGETESPVSQQTFTSDLVEGRAIDPFFALKEAERLRGESG
                     KEMALARLLARAGLGFKARYRWDEAKQALTEAVELHRRFGAETEEFADLLAALADVLK
                     WKTDYEQAVDILLQALRIFQQSGNVHGEANCILTLGYIALDRSLHDEARARFKEALPL
                     YRQVGGVLGEANCILSLGNIALNRSQHDEARARFKEALVLYQQIGNVLGEANCIKSIG
                     DIALRRSQYDEARAHFEEALPLYRQFGDVLGEANCIQSLGDIALEQGERDEAKCSFIE
                     ARELFEQIPEPYSIGRARRRLARIADDESERQQHLQAARAAWESIGFSELVEGLDREF
                     STSE"
     gene            9113..10495
                     /gene="hydA"
                     /locus_tag="VGB76_02520"
     CDS             9113..10495
                     /gene="hydA"
                     /locus_tag="VGB76_02520"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY0097804.1"
                     /translation="MKTLIRNGRVVTAVDDYKADILIEGETVSVIGARLDVEADVVID
                     AGGKLVIPGGIDPHTHMELPFGGTQASDDFFTGTRAAAYGGTTMIIDFAVQYKGESLT
                     EALDNWHAKAEGKTAIDYSFHLITTDLEDDRVPELHRIIDEGVTSFKMFMAYPGVFLV
                     DDATIFRAMSAAGERGGLICMHAENGIVINEIIKRALAAGRTAPKYHALTRPTRAEAE
                     GVHRAIAIAEMAESPVYIVHLSCADALEQVRAARDRGLPAFAETCPQYLFLSTDNYDE
                     PGFEGAKYVMTPPLRERWNQAELWKGLKTDDLQVISTDHCPFCMKEQKELGREDFSKI
                     PNGAPGVEHRVPLIYNGGVVENRLSLNRFVELTSTAAAKMFGLFPKKGTIAVGSDADI
                     VIFDAEREQTISAATHHMNVDYSAYEGKRIKGVVETVLSRGRVVIENGEYKGKAGDGR
                     FIKRGTCVTM"
     gene            10564..11289
                     /locus_tag="VGB76_02525"
     CDS             10564..11289
                     /locus_tag="VGB76_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097805.1"
                     /translation="MNHKIKYLALPLCLILLTTPACNRDGIGASSGGATLPGSGVNNS
                     GGTTTSAAISETDKPLDVMTRAMRAQLDAKSYRAHVTTTLADNAPNKMIIEYVAPDRY
                     RMVNDMQVGGKSVSQEFIIVGNGSYIKAPNGQWMRSPVDASEIVKGFRDPKMLDELAK
                     TADVKFVGADVIGGAPMLVYQYTQNNPMGMQLKSVSKTWLSVADGLPRKTESEGEFQG
                     KKTKTLVSISDYNADIKIESPIK"
     gene            11300..12037
                     /locus_tag="VGB76_02530"
     CDS             11300..12037
                     /locus_tag="VGB76_02530"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY0097806.1"
                     /translation="MYRSLRNISRTALVGLCLASVVAAQPQREPPLITVTGESEVRVV
                     PDEVVFDLTVQTLNRDLRLAKAQTDERLKKTLDLTRKYRIAPADVQTDYVKLEPRYRS
                     NDETRTLVGYSVRKDLVFTLRDASQAEALLSEVIETGITRINSIVFRSSQLRKHRDQA
                     RALAIKAAQEKAAALAAEIGQKIGKAYSIEEEGAGGSTRFNISGSAGSQNSFSTDTDR
                     SSQESEGTLALGQIKVSARVTVRFYLD"
     gene            12049..13575
                     /locus_tag="VGB76_02535"
     CDS             12049..13575
                     /locus_tag="VGB76_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEY0097807.1"
                     /translation="MATVTGSEDVYVTQRDFEELREDVSASPLWNRDLAPTTIRERTW
                     STWNIAALWIGMSVVITTYTLAGAFIEAGMSWWQAMITILLGNTIVLIPMILNAHAGT
                     KYGVSFPVLCRASFGVRGANVPAILRAIVACGWFGIQTWIGGTALDALFGAMWGGWNN
                     LFGGGGVDGVAWHTWIGFFLFWLIQVFIILKGVEGIKHLETWSAPLLLAGGAALLAWA
                     AWRAGGLGRVLSESTALQKQHNNFWTIFPGALTASVGYWATLSLNIPDFTRYARSQRS
                     QMTGQALGLPLTMTAFAFIGVAVTSATLLIYGVAIPNPVELMQRFDSVAIILFATLVI
                     FAAQLTTNMAANVVSPSNDFSNLNPKLISYVMGGIITALIGVLMMPWKLMTGSMGAYI
                     FTWLIGYSGLMGAIAGILICDYWVLRRQHLDLHDLFQLKGRYTYNAGFNLRALIALVV
                     AIAPVVPGFLHAANTPGGQIAQPGFFDTLYTYAWFVTFAIGFVLYYLLMQGNLRKEES
                     "
     gene            13672..14541
                     /locus_tag="VGB76_02540"
     CDS             13672..14541
                     /locus_tag="VGB76_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY0097808.1"
                     /translation="MPRTVKCGLIQATNAAPTDAPIEEIKRANIEKHLALIADAAKQG
                     VQILCMQEVFTTPYFCAEQQTRWYEAVEKIPDGPTVRLMQEVAKQYGMVIVVPIYEEE
                     IVGIYYNTAAVIDADGKYLGKYRKNHIPHVNPGFWEKFYFKPGNLGYPAFDTAYARIG
                     VYICYDRHFPEGARILGLNGAEIVFNPSATVAGLSEYLWRLEQPAHAVANGYFIGAIN
                     RVGHEQPWDIGEFYGQSYFCDPRGKFLAEAPRDEDALVVAELDLDMIREVRNTWQFFR
                     DRRPDTYDQIVAD"
     gene            14637..16055
                     /locus_tag="VGB76_02545"
     CDS             14637..16055
                     /locus_tag="VGB76_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0097809.1"
                     /translation="MSTTHDAPLNLAQIERNFREIAPPLTEAEAIMESHRCIYCYDAP
                     CTRACPTHIDVPAFIKKIASGNLTGSARVIFDANPIGATCARVCPVEVLCEGACVEKT
                     LMAKPIEIGRLQRYATDAAFARGRDVLKAGAPNGKSIGIIGSGPAGLSCATYLARLGY
                     AVTIYERKPLAGGMDTYGMAEYKMTQRVSQDEVALVARLGVEFKTGTEVGRDVSLKEL
                     EAAHDAIFLGIGLGATARLKIPGEQLSGVYDALDFIERIKTRDWASVPIGRTVAVIGA
                     GNTAIDAVTQAKRLGAERVLLIYRRTQHEMPAYEYEYELAKRDGIEFWWQTAPVEILP
                     GADGTRAAMLRCVRTEAVETGEAGRRSFQIVAGSEFDIPVDMVVKALGQQKMTQWLSE
                     QAQVKYDESGRVVVNPETMQTSNAKFFAGGDCVNGGREAVDASQAGKLAAQGIHEKLS
                     GERVRFAGAQVPLHEETSEIKH"
     gene            16087..17355
                     /gene="preA"
                     /locus_tag="VGB76_02550"
     CDS             16087..17355
                     /gene="preA"
                     /locus_tag="VGB76_02550"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEY0097810.1"
                     /translation="MADLKVNFAGIESPNPFWLASAPPTNMGSMVERAFDAGWGGAVW
                     KTLGEPIRNVCSRLASIDQGSQRMLGLNNIELITDRPLEVNLREIKECKRKYPKHALI
                     ISLMVESKREAWHEIAKQVEDTGADGVELNFGCPHGMSERGMGAAMGQVPEYTCMVTE
                     WVKEATRLPVIVKLTPNVTNITPPGRAAVRGGADAVSLINTINSVMGVDLDTLIPHPN
                     VNGMAAHGGYCGPAVKPIALNMVSELARDAEVNVPISGIGGISTWRDATEFLLLGATT
                     VQVCTAVMHYGYRIVEDMIDGLSNYLDDKGFLSVNDIIGRSVPRVTDWGNLDLNYKTV
                     ARIDHNKCIQCNLCHIACEDGAHQCIPFTEIAGKRFPIVDEHECVGCNLCHLVCPVPG
                     CISMVRLDDGTRPLTWRELTAREPETMTAD"
     gene            17428..>18540
                     /gene="rmuC"
                     /locus_tag="VGB76_02555"
     CDS             17428..>18540
                     /gene="rmuC"
                     /locus_tag="VGB76_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY0097811.1"
                     /translation="MEALLLVILIFLVLAVLAIAIKAAMRSPQGTEDISIPLQNLTNL
                     INQSQTRIEVLSEKLSQVERKQDSVGQHVVSMGTGLAKTGSVAESLVDATNFIRDELS
                     RAKNDLTALQSHTKARESIERDTAESIKRLEAVIAGTHSKGMAGENILEIMFARLPAE
                     WQMRNFTVGNKTVEFGLRLPNNRILPIDSKWTATHLLEQFISCQDVNEQLSLKGKIEK
                     AVLDKAKEVQKYIDTNLTVNFGVAVIPDAIYELCCGIHCDAFKTKVVLVSHSMFIPYL
                     LLVIESAHKDSQSIDEVKLRDYLKTTQSTIESLQREIEKRFSLALTMLDNSRTEIRGQ
                     LSKMSSGITGLQIGVNASQIEESQLPTLLDVVESSAA"
BASE COUNT         4004 a         5526 c         5390 g         3620 t
ORIGIN      
        1 cgggtgcctt cgtgttcata gttgaagata aacacctttt cgcttccggt gtttgtttgc
       61 aattgcaggc ttcccgcacc ggcgggcggc gtcgcaggac cgttcacgaa cgcgccggcg
      121 gtgggcgcag ttccggtgcg cgtgtagagc acccagtcgt ctgcggcagt cggcggggtg
      181 ctctccggac gccgggcaat gtcgccttca gtcacgacat ctgtggtaca cgtttcgtcc
      241 acggcgagtt tggcgacggg ggtatagacg agcgcggcga gcgcggcaac aagggcaaga
      301 acaaacaatt tggtcacttt catctgtact ctattctcct aaggggcaga aggttgcgcg
      361 gaaatgcgca acgtcggggg ttaaagtttg agaagccaca aacacagact cctacggctc
      421 agagcgactg acacacttcg cgccaagcaa aaaccgggtg ggcatgtcgc gcacaacacc
      481 acacggttca agcctgtctg accggcacaa aattagtagt cacattcgga ttgttgtctg
      541 tcggatggaa ggaaaaactt cgcggccttc gcggggacag agctaagtag acggggcatc
      601 tccagacacg tgcggccagc agcgaaagga gatgatcagg cgtcttttga taatgctcgc
      661 ggactttaaa gcaacgctgt taatgatgtc aacttggtac ggccttcata aatgacgaaa
      721 gcgcgatcaa ttccagcgtc actctgagcg gttgaacttt cgggcaagtt cgcgcttgag
      781 cacttttccg gtcgcgcctt tcggcaactc cgggaggaag cggatcgtct tcgggcattt
      841 gaagtcggcg agatgtgccc ggcaaaactc catgatctcc gcgggcgagg cttcgcgccc
      901 ctctttcaac acgacgaaag cggccacctc ttcaccgtac aaattatcgg gcacgccgat
      961 ggtcgcggcg gaagcgatag ccgggtgctt gtagagcagt tcgtcgatct cgcgcgggta
     1021 gatattttca ccgccgcgaa tgatcatgtc cgacatccga tccacgatgt agaagaatcc
     1081 atcggcgtcg cggtagccga catcgcccgt gtgaaaccag ccgccgcgaa aagcctctct
     1141 cgtggccggt tcgttttcgt agtagccttt aaagatgttc tcgccgcgaa gcacgatctg
     1201 gccgagttcg cccgtggcga cttccctctc cgcttcgtcg aagaccttga tctcgcagcc
     1261 gattgattgc ccgcacgagc cgggacgacg gcgcgcgtcc ggcgggttga acgtcgcccg
     1321 cacggttgat tccgacaacc cgtagccttc gatgacgaga caacggaaag tctcttcgaa
     1381 gcgacgcagg acttccacgg gcacgggcgc ggagccgcac atcgcaaagc gcagttgctc
     1441 cgtgtgcaga ccgccgggca cgccctccgt gtaagtggcg agcagcatgg agagcatcgt
     1501 ggcgaccgag ccgaacgagg tcactccgta atcggagatg gtctgccaga agcgcgacgc
     1561 cgagaagcgc gggctgacga cgctcgaccc gcccgcgtac atcgccgcca cggtcgtcac
     1621 catcacggcg ttcatgtgaa agagcggcat cacggagagc agtcgatcct ccggcgtgaa
     1681 cttcatccac tccacgatct gccgcgcgtt cgccagcaaa tttccgtgcg tcaggagaca
     1741 gcctttcggc tttccggtcg tgcccgaagt gtagatgatg atcgcttcgt cgtcgcggct
     1801 caaggcgagt tcgcgccacg actcgccggg cgtggcggcg agatttgcgt tagcagtcgt
     1861 ctcaacgtca aagatgagcg cggcggcgag gcgcggcacc tccttttgta tctccgtcac
     1921 agtttcgcga aactgcgaat gatagagcag agctttcgcc ttcgaattgc cgacgacgta
     1981 agccgcctct tccggcttga gcagagagtt gaccggacct gccagcgcgc cggtcttgaa
     2041 gcaggcgaag taggcgatga tgtattccgc gctgttcggc aagagcaggc tcaccacgtc
     2101 gccgcgcccg acgccctgcg ccgcgagcag gttggccgtc cggttgaccg ccgcatcaaa
     2161 ctccgcgtaa gtccaactgc gcccgtcgtg cgcgtcgaag agaaaagttt tggcgggcgt
     2221 ctcggaggcg cgtcgttcca gcagttggcg catgttggcg tcaggttcgg gcaaacgggt
     2281 ggtcttcata attcagattg caaggcggcg cggctgtgcg gtataaagtc cgggtaaact
     2341 gaggcgtcgc tatttacttc tgcgtccgcg ccgaacgcgc cacagcttaa cgaaacgctt
     2401 ccatctctac aattatttcc gttccggcat tgctgctgtt cgcccccgca accagacgcc
     2461 tcgcgcatgg tcggcacgcg cgaccgaaat gctttcttat ggacacctat cgtaatttta
     2521 ttgaaggcca gtggattctc tccgaggccg cgcagacggt tcccaatctc aacccggcca
     2581 atacggacga cgtgctcggc acagtgcgcc tctccctgcg cgaggaagcg cgtcgcgccg
     2641 tcgaggttgc acacgccgcg tttcgggagt ggcgcgcgac gcctgcgccc gcgcgcggac
     2701 gcatcatcgc acgtttcgca cgcctgctcg aagaggacac ggagaatctc gccgccctgc
     2761 tcacgcggga ggagggcaag acgctcgccg aatcgcgcgg cgagatacga cgcgccatca
     2821 acgtcgcgga gttttgcgcg ggcgaagcga ggcgcatgaa cggcgagacg atccaatcgg
     2881 aactccccgc caacttcgcc tacaccatca agcagccgca cggcgtcgtc gcctgcgtca
     2941 cgccgtggaa cttccccgtc gccattcccg tctggaagat cgcgcccgcg ctcgtcgcag
     3001 gcaacacggt gatcttcaaa ccggcgaccg tgacgcccgc caccgccgtg cgcctcgtcg
     3061 aactcttcgc ggaagcgggc ttgccgaagg gcgtcctgaa tctcgtcatc ggctccggct
     3121 cggaggcggg cgacgagatc gtgaatcacc cggcggtgcg cgcagtctcc ttcaccggct
     3181 cgaaccccat cggcctcaag ctctacgagc agtgcgcgcg gcgcggcgcg aaagtgcaat
     3241 gcgagatggg cggcaagaac cctgtgatcg tgctcgaaga cgccgacatg aacctcgccg
     3301 tcgaatcgac cgcacagggc gcgttcggct caaccggcca acgctgcacg gcgacgagcc
     3361 gcgccatcgt gcatgaaaaa gtttacgaca acttcatcga gcgcatcacg gcgcgggtgg
     3421 agagtttgcg cctcggcgac ggcagccagc ccgacacgga agtggggcca ctcgttgacg
     3481 gcaaggcgtt caagagcgtg ctctcctaca tggacatcgg gcgcgaagac ggcgcgagtc
     3541 tggtgtgcgg cggcgcaatc gccgaagggc cgggcttgcg caaaggctac ttcgtcaagc
     3601 cgacgatctt cgtcggcgtc acgcccgaca tgcgtatcgc ccgcgaagag attttcggtc
     3661 ccgtcctctc cgtcatgcgc gccaaagatt tagacgaagc gctcctgatc gccaacgaca
     3721 gcgaatacgg cctctcctca tccatcttca ccaacgacgc ggcacgcatc ttccgcttcg
     3781 ttgacgagat cgaaaccggc atgacgcata tcaactcgcc cacgaccggc ggcgaagcgc
     3841 acatcccttt cggcggcatc aaggccaccg gcatcggcga acgcgaacaa ggctcgaccg
     3901 ccctcgattt ttataccgaa ctcaaagtcg tctacgtgga ttacacggga cgcaaacggg
     3961 aaggaaactt gtactaaccg cagaggccaa cggacaaacg agtctgtgtc tgtagctact
     4021 tgcagccctg tcagattatg aacaccgttg tctgcactgc ttgtaaaacc gtgaacgcgg
     4081 aggctaacgt caattccgct tgctcacgtt gcggcgaatc gctcgcccct gcgctcatcg
     4141 aacagagcgt caacgagttg aagcgtctga cggcgaagtt ggaggaggcc agcgcccctt
     4201 cgttcaactc attcaacggc ttcgggacga cgctgctgga ttatcgcgcc agccccgtcg
     4261 gaacttacga agcgacgcgc tgggcaatcg cactctttct gccgctcttt cccattgcaa
     4321 actatgtgat ccaaccgacc gggcaggaga ggtcttacgg gcgcgaaacg tcgaagtttc
     4381 ggatcatggc gcagacgcct ttgacactga cgagaatcct gcgcacctac gcgctcgccg
     4441 tcgcgggact cttacccatc atcgccggtt ctctctactc caacgttgtt aaccgcacgc
     4501 tcggcggcct ccccgccgcc gttgccatga tcttatcttt cgtgtgggcg atttacatta
     4561 tcttcttcaa gttgaagaat gagggcgcgg cttacaagtc tgtcgccgcc acttgaaaac
     4621 ttccacccgt cccttaactt ttccacggag tctatcttta tgagcactgt cgagcagcaa
     4681 gtcgcgcaat ccgagatcgt acgcaagcac aaagagtttc tcttccccgc cgtcgccacc
     4741 tattatcaag agccggtcgc gctcgtgcgc ggcgaggggc aatacgtctg ggacgatcag
     4801 ggcacgcgct atctcgattg cttcggcggt gtgctcacgg tcagcgtcgg ccacgccaac
     4861 ccgcaggtca acgcggcgat catcgaacag gtcaagaccc tctcgcacac ttcgacgctc
     4921 tacgccaacc gaccacaggg cgacctcgcc gaaaagctcc accagatcac gcccgggcgt
     4981 ctgaagaaat ccttcttcac caacagcggc acggaagccg acgacacggc catcaccgcc
     5041 gcgaagattc acaccggacg ccacgagatc gtcgtcttgc gccacagcta ttcgggacgc
     5101 tccgccactg cgctctccgc catcggccat tccacctggc gaccgctccc cgcgcaagtc
     5161 gccgggatcg tccacgcacg cgcgccctac tgttatcgct gcccgctcaa gttgagctac
     5221 ccggaatgcg gactcgcctg cgccgaagac atcaaggacg tgatcgagac gacgaccacc
     5281 gggcagatcg ccgccttcat ggccgaaccg atcctcggcg tcggcggctt catcgtcccg
     5341 cccgccggat acttcgagcg cgccgcagag atcacgcgcg ccgccggcgg actcttcatc
     5401 gcagacgaag tgcagaccgg atgggggcgc acgggcgaca agtggttcgg catcgaacac
     5461 tggaacgtcg aacccgacat catcacctcg gccaaaggca tgggcaacgg cgtccccatc
     5521 ggcatgacca tcgccacgcc ggaggtggca gacagtttcc ccggcctcac cttctccacc
     5581 ttcggcggca accccgtctc gatggccgcc gcgctcgccg tgatcaaggt tatcgaagat
     5641 gagaacctgc gcacgaacgc cgccaccgtc ggcgcatact tccgcgaaaa actggaagga
     5701 ctcaaagaaa aatatcccgt catcggcgac gtgcgcggca tgggcttaat gcagggactc
     5761 gaactcgtcc gtgaccgcga gacgaaagaa cccgacccgc aatccctcct cagcgttttc
     5821 gaggaaacca aacgtcgcgg cgtcctcatc ggcaaaggcg gcctctacgc caacgtcatc
     5881 cgcaccggcc tcatgctcaa ctccacgaag gacacggttg acgaactgat aaccgccctc
     5941 gatgcaggtc tggcagcgtt atgatgttgt aattgcgacg cctggtgttt gccgctacaa
     6001 tcaggctaag aacatatcat cactaccgtt gaaaatttat ggcattgact ccatcagata
     6061 aagaattgct caagggaatc taccggaagg tgaaagacga gccgcttaaa ccggggacgc
     6121 cagaatttga gcgattctac cagccaattt acgaaattcc cggtaaggaa gacccggtgg
     6181 atttcttaca gaaggctatt cagttcagcg aatatgagag tctccagctt ttttcaggct
     6241 tccgaggctc aggcaagacc accgaattgt tccggctcag gcggcgtctg gaacaagagg
     6301 gctacgtcgt gctgtatgtc gatgccctca agtacatcag cccttcggac gagattgata
     6361 tatccgacct gttgattgtg ctcgccggag cattcagcga cacgctcgaa gaatgggcgg
     6421 cgcagttgca acaacctgtc cagttggcgc atgagactta ctggaaccgc atcaagaatt
     6481 ttttgacgaa aacctctgtc gagttgaacg aggcgacagt ttcgctcgga ggcgagactc
     6541 ctgccggaca gattctcggg agccttaaag gcgggctgga tttgaagctc gcaatcaagg
     6601 agtcaccgac ctttcgccag aacttgcaaa agttgatggc gaaccgcatc ggtgaactgc
     6661 gcgccaccgt gagtcaattc ttcgaggatg gcgtgaaggc agtcaagcag gtgtggggcg
     6721 atgagaagga ggtggtttac atcttcgatt cactcgaaca gttgcgcggc gctctcacca
     6781 atgaagaggg cgtgctgaaa agcgtgcaac gcgtctttgc cagccacctc aatctgttgc
     6841 gcctgccgta cattcacgtc atctacaccg ttccgccgtg gctccagttc gtgatgcaag
     6901 gcagcgacat ctacctcctg ccctgcattc ggctatggaa taatgacgcg gaacgcacgc
     6961 attatgaacc gggctggaac gctgtgcgct ctctggtgtt taaacggatc gggcaggagg
     7021 gctgcgagcg gctctttggc tgcaagctgg accagccctt cacttcagct gacgatctga
     7081 tcgccgtctg cggcggccac ttccgcgatc tgttgcgact gctgggcgac atcgtccgcc
     7141 gcgccgactc gctcccgatc acgccggacg tgcttaacag tgccgtcatg tctctgcgcg
     7201 acgactactc gccgattgcc atcgaagacg cgctctggct ggcgaagatc ggcgagcacc
     7261 gcacaccgat gttgcccgat acgaaatcgc agacgattag ccgcctgaca cgtttcctcg
     7321 acacacatgt cgtccttcac ctgaccaatg gcgtcaaatg gtacgacatt cacccactgg
     7381 tcagagatga ggtggcaaag ctcgtcaagt tccagcaaga ctctcagctt ctgaagaatg
     7441 ctcaacccgt gggcggcgcg ccggcagaca gatcgcctca accatgacga agaccgagac
     7501 atccttaccc actctgccag cgcagcgttc aggcgtcgca gcctcgcccg aatggcgaag
     7561 cctgctcacc cacttcgaat tggctcccga cgacttcgcc ttcatcgtgc tgctcgtgcc
     7621 ggacagagat tgggcggaag cctgccgtca agccctcgaa cgtttcctga tctcttcacg
     7681 caaaaagctg ctggccgttc acttcgaaga tgcgcgcgag ttcagagacg aattggcgac
     7741 gcgcctgctg tcgctgcaag cgggcgacga catcggcgtg gtctgggtaa gcgaagcggt
     7801 gacggaagcc gccgccgact atgacgagtg ggcgcaggct tggcgcgttg cggcgggacg
     7861 gctcaatcaa tatcgcaacc ctttacgccg ccagttccac gtcccgctga ttttcgctgg
     7921 cgcgccgtgg attcaggtga cgcttcgcga ggcggctccc gatttatggt ctgtgcggac
     7981 acaggtagtg cggatcatac ctcccgccgc cgaggatagg ggagagactg aatcccccgt
     8041 gtctcaacaa acgttcacat cggacttggt agagggacgg gcaatcgatc cctttttcgc
     8101 tttgaaagag gcggaacgct tgcgcggcga aagcggcaag gagatggctt tagcgcgtct
     8161 actcgcgcgc gcgggacttg gattcaaggc tcggtatcgt tgggatgaag caaaacaggc
     8221 attgaccgag gccgttgaat tacaccgccg ctttggagcg gaaacagaag agtttgccga
     8281 tttattggct gctcttgccg atgtccttaa atggaagaca gattacgagc aagctgtaga
     8341 tattcttctg caagcattaa ggatatttca acaatcaggc aacgtgcatg gcgaagccaa
     8401 ttgtatcttg actctcggct acatcgcgct tgacagatcg ctgcacgatg aagcgcgcgc
     8461 acgcttcaag gaagcgttgc cgctctatcg tcaggttgga ggtgtgctcg gcgaggccaa
     8521 ttgcatcttg agtctcggca acatcgcgct caatagatca cagcatgatg aagcgcgcgc
     8581 ccgcttcaag gaagcattag ttctttatca acagatcggc aacgtgctcg gcgaggccaa
     8641 ctgcattaaa agtattggcg atatcgcgct tcgcagatcg caatacgatg aagcgcgcgc
     8701 ccacttcgag gaagcgttgc cactctatcg tcagttcggc gatgtgcttg gcgaggccaa
     8761 ctgcatccag agtctcggcg acatcgcttt agagcagggt gaaagagatg aggctaagtg
     8821 ctcctttatc gaagcccgtg agttatttga gcagattccg gaaccatatt caatcggtcg
     8881 cgcgcggcgg cgattggctc ggattgcaga tgatgagtct gagcgtcagc agcacttaca
     8941 ggctgcccgc gccgcgtggg aaagtatcgg cttctcagag ttggtagaag ggttggacag
     9001 ggaattcagc acatccgaat aaccgttact tgtcggacga acggcctttc gcctgtgacg
     9061 tattaacaac attccggcag attaaggctt ctctttattc aagtgtcaat ttatgaaaac
     9121 tctcattcgt aatggtcgcg tcgtcacagc cgttgacgat tacaaggccg acatattaat
     9181 cgaaggcgag acggtgtcgg tgatcggcgc aaggctcgac gtggaagcgg acgtagtgat
     9241 tgatgcgggg ggaaagttgg tcatccccgg tgggatcgat ccgcatacgc acatggaact
     9301 tccctttggg gggacgcagg cgtcggatga tttctttacg gggacgcggg cggcggctta
     9361 cggcgggacg acgatgatca ttgatttcgc ggtgcagtac aagggcgagt cgctgacgga
     9421 ggctctggat aactggcacg cgaaggccga gggcaagacg gcgattgatt acagttttca
     9481 tttgatcacg accgatctgg aagacgaccg cgtgccggag ttgcaccgga tcattgacga
     9541 gggcgtgacg agtttcaaga tgttcatggc ctacccgggc gtctttctcg tggacgatgc
     9601 gacgatcttt cgcgcgatgt cggcggcggg cgagcgcggc ggtttgatct gcatgcacgc
     9661 ggagaacggc atcgtcatca acgagatcat caagcgcgcg ctcgccgcag ggcgcaccgc
     9721 gccgaagtat catgcgctga cgcgaccgac gcgcgccgag gccgaaggcg tgcatcgcgc
     9781 gattgcgatt gcagagatgg ccgagtcgcc cgtctacatc gtgcacttga gttgcgccga
     9841 tgcgctcgaa caggtgcgcg cggcgcgcga ccgtggactc cctgcgttcg cagagacatg
     9901 cccgcaatat ctcttcctct cgaccgacaa ttacgacgag ccgggattcg agggcgcgaa
     9961 gtatgtgatg acgccaccgc tcagggagcg ttggaatcag gccgagttgt ggaagggttt
    10021 gaagacggac gacctgcaag tgatctccac agaccactgc cctttctgca tgaaggagca
    10081 gaaggaactc gggcgcgaag acttttccaa aatcccgaac ggcgcgcccg gcgtcgagca
    10141 tcgcgtgccg ctcatctaca acggcggcgt cgtcgaaaat cgcctctcgc tcaaccgctt
    10201 cgtcgaactc acttccaccg ccgccgccaa gatgttcggc ctcttcccga agaaaggcac
    10261 gattgccgtc gggtctgacg ctgacatcgt tatcttcgac gcggaacgcg aacagacgat
    10321 cagcgcggcg acgcatcaca tgaacgttga ctacagcgcg tatgaaggca agcgtatcaa
    10381 gggcgtcgtc gagacggtct tgtcgcgcgg tcgcgtcgtc atcgaaaacg gagagtacaa
    10441 gggcaaggcg ggtgatggcc ggttcatcaa gcgcggaacc tgtgtcacga tgtaagcaat
    10501 cctctttgtc tgttttcttt caagcgggaa acttcaacca accaagccgg gaggcgattt
    10561 cccatgaatc ataagattaa atatctggct ctgccgctct gccttatttt gctgacgacc
    10621 cctgcctgca acagagacgg aattggcgcg agcagcggcg gcgcaacgct acccggtagc
    10681 ggcgtcaaca acagcggcgg cacgacgaca agcgccgcca tctccgaaac cgacaagccg
    10741 ctcgacgtga tgaccagagc gatgcgcgcc caactcgacg cgaaatccta tcgcgcgcac
    10801 gtcaccacta cgctcgccga caatgctccc aacaagatga tcatcgaata cgtcgcgccc
    10861 gaccgctacc gcatggtcaa cgatatgcag gtcggcggca aaagcgtcag ccaggagttc
    10921 atcatcgtgg gcaacggctc atatatcaaa gctccgaacg gccagtggat gagatcgccg
    10981 gttgacgcga gcgagatcgt caaggggttt cgcgatccga aaatgctgga cgagttggcg
    11041 aagacggcgg acgttaaatt cgtcggggcg gacgtaatcg gcggcgcgcc catgctcgtc
    11101 tatcaataca cgcagaacaa cccgatgggg atgcaactca agagcgtctc gaagacgtgg
    11161 ctctccgtcg ccgacgggct gccgcgcaag accgaaagtg aaggcgagtt tcagggtaag
    11221 aagacgaaga cgctcgtgtc gatctccgat tacaacgccg acatcaagat cgaatccccc
    11281 atcaagtgaa ggaggccgca tgtatcgttc tttgcgaaac atatcgagaa ccgcgctcgt
    11341 cggcctctgt ctggcttccg tcgtcgcggc gcaaccgcag cgcgagccgc cgctcatcac
    11401 cgtgacgggc gaatcggaag tgcgcgtcgt gccggatgaa gtggtcttcg atctcaccgt
    11461 gcagacgctc aaccgtgatc tgcgtctggc gaaagcgcag accgatgaaa gactcaaaaa
    11521 gactctggac ttgacgcgca agtacaggat cgcccccgcc gacgtgcaga ccgactacgt
    11581 taaactcgaa ccgcgttatc gcagcaacga cgagacgaga acgctcgtcg gttattccgt
    11641 caggaaagac ctcgtcttca cgctgcgcga cgcttcgcag gccgaagcat tgctctcgga
    11701 agtgattgaa acgggcatca cgcgcatcaa cagcatcgtc ttccgttcgt cgcaactgcg
    11761 caagcacagg gatcaggcgc gcgcgctcgc catcaaagcc gcgcaggaga aggccgccgc
    11821 gctcgccgct gagatcgggc agaagatcgg gaaggcttat tccatcgagg aagaaggcgc
    11881 gggaggttcg actcgtttca acatttccgg cagcgcgggc agtcagaact ccttcagcac
    11941 cgacaccgac cggagttcgc aggagtcgga aggcacgctc gcgctcggcc agatcaaagt
    12001 gagcgcgcgc gtcacggttc gtttctacct cgattagaga tacggcttat ggcgactgtc
    12061 accggtagcg aagatgtgta cgtcacgcag cgcgacttcg aggagttgcg cgaagacgtg
    12121 tccgccagtc ccctgtggaa ccgcgacctc gcgccgacaa ccatccgcga gcgcacgtgg
    12181 tcaacgtgga acatcgccgc gctgtggatc gggatgagcg tcgtcatcac gacctacacg
    12241 ctggccgggg cgttcatcga agcgggcatg agttggtggc aggcgatgat cacgatcctg
    12301 ctcggcaaca ccatcgtcct catcccgatg atcctgaacg cgcacgcggg cacgaagtac
    12361 ggcgtctctt ttccggtgct ctgccgcgct tcgttcggag tgcgcggcgc gaacgtcccg
    12421 gcgatcctgc gcgccattgt cgcgtgcggc tggttcggca tccagacatg gatcggcggc
    12481 accgccctcg atgcgctgtt cggcgcgatg tggggcgggt ggaacaacct gttcggcggc
    12541 gggggcgtgg acggcgttgc gtggcacacg tggatcgggt tcttcctctt ctggctgatt
    12601 caggtcttca tcatcctgaa gggcgtcgaa ggcatcaagc atctggagac gtggtcggct
    12661 cctttgctgc tcgcgggcgg cgcggcgttg ctcgcgtggg ctgcgtggcg cgcgggcgga
    12721 ctcgggcgcg tcctctccga atcaaccgcc ttgcagaaac agcacaacaa cttctggacg
    12781 atctttcccg gcgcgctcac cgcttcggtc ggctactggg cgacgctcag cctcaacatc
    12841 cccgacttca cgcgctacgc ccgctcgcag agatcgcaga tgacaggtca ggctttgggg
    12901 ctgccgctga cgatgaccgc gtttgctttc atcggcgtcg ccgtgacgag cgcgaccttg
    12961 ttgatttacg gcgtggcgat tcccaacccg gtcgagttga tgcaacgctt cgacagcgtc
    13021 gccatcatcc tcttcgccac gctcgtcatc ttcgccgcgc aactcacgac caacatggcc
    13081 gccaacgtcg tctcgccctc gaacgacttt tcgaacctca atccgaaact gatctcctac
    13141 gtgatgggcg gcatcatcac cgcgctcatc ggcgtgctga tgatgccttg gaaattgatg
    13201 acgggttcga tgggcgcgta tatcttcacc tggctcatcg gctattccgg cttgatggga
    13261 gccatcgcgg gcattctcat ctgcgactac tgggtcttac gccgccaaca cctcgacctg
    13321 catgatctgt ttcaactcaa aggccgctat acttacaatg caggcttcaa cctgcgcgcg
    13381 ctcatcgccc tcgtcgttgc catcgcgccg gtcgtcccgg gctttctgca cgcggccaac
    13441 actcccggcg ggcagatcgc gcaacccggc tttttcgaca cgctctacac ctacgcatgg
    13501 ttcgtcacct tcgcgatagg cttcgtgctt tactatctgt taatgcaggg aaacttgaga
    13561 aaggaagaaa gttgagtgat gagtgatgag tgataagtga tgagttaaga agcaactgtc
    13621 tttaaactca tcactcatca ctcacactca tcactcgcct ttgtaagaat tatgccacgc
    13681 acagttaaat gcggactcat acaggcgacg aacgccgcgc ccacggacgc gcccatcgaa
    13741 gagatcaagc gcgcgaatat cgagaagcac ctggcgttga tcgcagatgc ggccaaacag
    13801 ggcgtgcaga ttctctgcat gcaggaagtt ttcaccacgc cttatttctg cgccgagcag
    13861 cagacgcgct ggtatgaggc ggtggagaaa attcccgacg gcccgacggt caggttgatg
    13921 caggaggtgg cgaagcaata cggcatggtc atcgtcgtgc cgatctacga agaagagatc
    13981 gtggggattt attacaacac cgccgccgtt atagacgccg acggaaaata tctcggcaag
    14041 tatcgcaaga accacattcc gcacgtcaat ccgggcttct gggagaagtt ctacttcaag
    14101 ccgggcaatc tcggctaccc ggccttcgac acggcctacg cgcgcatcgg cgtctatatc
    14161 tgctacgacc gccacttccc cgaaggcgca cgcatcctcg gcttgaacgg cgcagagatc
    14221 gtcttcaacc cgtcggccac cgtcgccgga ctctccgaat acctctggcg tctggagcaa
    14281 cccgcacacg ccgtcgccaa cggctacttc atcggcgcga tcaatcgcgt cggccacgaa
    14341 caaccgtggg acatcggcga gttctacggc cagagttatt tctgcgaccc gcgtggtaaa
    14401 ttcctggccg aagccccgcg cgacgaggac gcactcgtcg tcgccgaact cgacctcgac
    14461 atgatccgcg aagtccgcaa cacgtggcaa ttcttccgcg accgcaggcc ggacacttac
    14521 gatcagatcg tggcagacta aacagagttc caagtctcaa atctcaagtc ccgcgtcgaa
    14581 cgcggcctgg cgacctggga ttgagacttg agacttgaaa cttgagactc gaacttatgt
    14641 caacgactca cgacgcgccg cttaacctcg cgcagatcga acgaaacttt cgggagatcg
    14701 cgcccccttt gaccgaggcc gaagcgatca tggaatcgca tcgctgcatc tattgctacg
    14761 acgcgccctg cacgcgcgcc tgcccgacgc acatcgacgt tcccgccttc atcaaaaaga
    14821 tcgccagcgg caatctcaca ggctctgcgc gcgtcatctt cgatgccaac ccgattggcg
    14881 cgacctgtgc gcgcgtctgc cccgtcgagg tgctctgcga aggcgcgtgc gtggagaaga
    14941 ccctcatggc gaagccgatt gagatcggtc gtttgcaacg ctacgcgacg gatgcagcct
    15001 tcgcacgcgg gcgcgacgtg ctcaaggcgg gcgcgccgaa cggcaaatca atcggcatca
    15061 tcggctctgg tccggcgggt ctgtcgtgtg cgacctatct ggcgcgtctc ggctatgccg
    15121 tgacgatcta cgagcgcaag ccgctcgcgg gcggcatgga cacctacggg atggccgaat
    15181 acaagatgac gcagcgcgtc tcacaggatg aagtggcgtt ggttgcgcgt ctcggcgtcg
    15241 aatttaaaac cggcacggaa gtcggacgcg acgtgtcgtt gaaagaactg gaagcggcgc
    15301 acgacgcgat ctttctcggc atcggactcg gcgcgacggc gcggctcaag attccgggcg
    15361 aacaactctc cggcgtctac gatgcgctcg atttcatcga acgcatcaag acgcgcgatt
    15421 gggcgagcgt tcccatcggg cgcacggtcg ccgtcatcgg cgcgggcaac accgcgattg
    15481 atgcggtcac gcaggccaaa cggctcggcg cggaacgcgt gctcctgatc taccgccgca
    15541 cgcagcacga gatgcccgct tacgagtatg aatacgaact ggcgaaacgt gacgggatcg
    15601 agttctggtg gcagaccgcg cccgtggaaa ttttgccggg cgcagacggc acgcgcgccg
    15661 ccatgcttcg ctgcgtcagg acggaagccg tcgagacggg cgaggcgggg cgtcgttcgt
    15721 ttcagattgt ggcgggttcg gagttcgaca ttccggtcga tatggtcgtc aaggcgctcg
    15781 gccagcagaa gatgacacag tggctctcgg aacaggctca ggttaagtac gacgagagcg
    15841 ggcgcgtcgt cgtcaaccct gagacgatgc agacgagcaa cgcgaagttc ttcgcgggcg
    15901 gcgactgcgt gaacggcggg cgcgaagcgg ttgacgcctc gcaagccggt aaactcgcgg
    15961 cgcagggcat ccacgaaaag ttgtcgggcg aacgcgtccg gttcgcgggc gcgcaggtgc
    16021 cgctccacga agagacgagc gagatcaagc actaaggaaa gcgttcagag ttgaggagtt
    16081 gaaatcatgg ccgatctgaa agttaacttt gcgggcatcg aatcgccgaa tccgttctgg
    16141 ctggcgtctg ctccgccgac gaatatgggt tcgatggtgg aacgcgcgtt tgacgcgggc
    16201 tggggcggcg cggtctggaa gactctcggc gaaccgatcc gcaacgtctg ctcgcgcctc
    16261 gcctcgattg atcaggggtc gcagcgcatg ctcggcctca acaacatcga actcatcacg
    16321 gatcgcccgc tcgaagtgaa cctcagagag atcaaggagt gcaaacgcaa gtatccgaag
    16381 cacgcgctga tcatctccct gatggtggaa tcgaagcgcg aggcgtggca cgagatcgcc
    16441 aagcaggtgg aagacacggg cgcggacggc gtggaactga acttcggctg cccgcacggc
    16501 atgtccgagc gcggcatggg cgcggcgatg gggcaggtgc ctgaatacac ctgcatggtg
    16561 acggagtggg tgaaggaagc gacgcggctt cccgtcatcg tcaaactcac gccgaacgtg
    16621 acgaacatca cgccgccggg tcgtgcggcg gtgcgtggcg gcgcggatgc cgtctcgctc
    16681 atcaacacga tcaactccgt gatgggcgtc gatctcgaca cgctcatccc gcacccgaac
    16741 gtcaacggca tggcggcgca cggcggttat tgtggtcccg ccgtcaaacc catcgcgctc
    16801 aacatggttt ccgaactcgc ccgcgacgcg gaagtgaacg tgcccatcag cggcatcggc
    16861 ggcatctcga cgtggcgcga cgcgacggag tttctcctcc tcggtgcaac gaccgtgcag
    16921 gtctgcaccg ccgtcatgca ctacggctac cgcattgtcg aagacatgat cgacggcctc
    16981 tcgaattatc tggacgataa aggcttcctc agtgtcaacg acatcatcgg gcggagcgtg
    17041 ccgcgcgtca ccgattgggg caacctcgat ctcaactaca agacggtcgc acgcattgac
    17101 cacaacaagt gcatccagtg caacctctgc cacatcgcct gcgaagacgg cgcgcaccag
    17161 tgtatcccct tcaccgagat tgcaggcaaa cgcttcccca tcgttgacga gcacgagtgc
    17221 gtcggctgca atctctgcca tctcgtctgc cccgtccccg gctgcatctc gatggtgcgc
    17281 ctcgacgacg gcacacgccc gctcacctgg cgcgaactca ccgcccgcga accggagacg
    17341 atgacggcgg actaaatata tttcaaccaa tacactctca cctaacttat gcttggcata
    17401 taactatcta aagaaggagt atcctcaatg gaagccctac tgcttgtaat cctcattttc
    17461 ttagtcttag ctgtgcttgc tattgctatc aaagcagcaa tgcgttcccc tcaaggtacg
    17521 gaagacatct caatcccact acaaaactta accaatttga tcaatcagag tcagacacgc
    17581 attgaggtgc tatctgaaaa actttcgcaa gtagaacgca agcaggatag cgtaggccag
    17641 cacgtcgttt cgatgggcac agggttggct aaaaccggat cagtcgctga aagtctcgtt
    17701 gatgcgacaa actttatacg tgacgaattg tctcgtgcga aaaacgattt aacggctcta
    17761 caatcccaca caaaagcgag ggagagtatt gaacgcgaca ctgccgagtc tatcaaacga
    17821 ttagaagccg taatcgctgg cacacacagt aaaggtatgg ctggtgaaaa catacttgaa
    17881 ataatgtttg ctagactccc agcggaatgg cagatgcgaa attttactgt tggcaataag
    17941 actgtagaat ttgggcttcg gttgccgaat aatcggattc tccctattga cagtaaatgg
    18001 acagccacgc acctactcga acagtttatt agttgtcagg atgttaatga gcaattatca
    18061 cttaaaggta aaattgagaa agcagtttta gacaaagcta aagaagttca aaaatatata
    18121 gacacaaact taactgttaa ttttggcgtc gcagttattc ccgatgcgat atatgaattg
    18181 tgctgtggta tccactgtga tgcttttaag acaaaggttg tacttgtcag tcacagcatg
    18241 ttcattccat atctcctttt ggtcatcgaa tctgctcaca aagattcaca aagtatcgat
    18301 gaggtcaaat tacgagacta cctaaaaaca acgcagagca ctattgagag tttgcagcgt
    18361 gagattgaaa agcgattctc tctggcgttg actatgcttg ataactcacg cacagaaata
    18421 cgtgggcagc tcagcaaaat gagcagtggt attacaggct tgcaaattgg ggtaaatgct
    18481 tcacaaattg aagaatcaca gttaccaact ttacttgatg ttgttgagtc gtcagcagca
//
LOCUS       DASWNS010000074         5828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_80590, whole genome shotgun sequence.
ACCESSION   DASWNS010000074 DASWNS010000000
VERSION     DASWNS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80590"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /gene="deoC"
                     /locus_tag="VGB76_02560"
     CDS             <1..697
                     /gene="deoC"
                     /locus_tag="VGB76_02560"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY0097812.1"
                     /translation="SARDWASLVDHTLLKPEASEADIERLCGEASQFHFASVCVNPTW
                     VRASACHLRGTGVVVCTVVGFPLGATLADVKAYETRRAIYDGAREVDMVINVGALKSG
                     DDCAVEYDIRQVVEAAHEHGVLVKVIIEAALLTDEEKVRACLAAKRAGADFVKTSTGF
                     AKGGATVADVALMRRTVGQEMGVKAAGGVKGLDDARAMVEAGATRLGASVGVKIAQEA
                     SGVRTATSSNGGY"
     gene            complement(894..1538)
                     /locus_tag="VGB76_02565"
     CDS             complement(894..1538)
                     /locus_tag="VGB76_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0097813.1"
                     /translation="MNKKPGCAAVAVLLCLFFASGATLAQTIETPRFEVGAQFSSLTL
                     ANRPSDTKPGFGGRFTVNLNDNVAVEAETNFYPTESTFPSEETGGRAVQGLFGVKAGK
                     RFDKFGIFAKARPGLVHFTRVLRVNGFTNEIIDGQSFIIPHFRTQGVTHFAADLGGVV
                     EFYPSRRIVTRFDIGDTIIRDSGYERPPFPGLVTFSNSATTHNFQFNAGIGFRF"
     gene            complement(1687..2847)
                     /locus_tag="VGB76_02570"
     CDS             complement(1687..2847)
                     /locus_tag="VGB76_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097814.1"
                     /translation="MNSKAKLSSSCSLALLLMLLCGIVAAPHALAQKGEAATERAGQF
                     VMPLHGEGFIAFRVEAAPLGTPGTNVNLRQIQAALTPQAMLADNHVVHRVLVDAEGNF
                     VFGYDLIVEPVAATKQFKVSVKPLSQEFEEKLRARQPAAGANRAPPNVSTLPRSGDAQ
                     FVDDGDAFALDLLVNAQTGVKIVDVVKVSFDRAKLWSVPPRRSVPLRDFTLGNVELAM
                     RDYKLTINGELVGGGQKPARGSAGALLWFYVPNRGRFIVSLMPHEGYDFQKVGRIENN
                     KISFTLGQDVYEWTSSAPVVGNGGNWNLWVLHDAGYVSDFASAEQIVEGREGKPASPE
                     WLQDPLARLRREGQRGEFETRTNDKAKRNAAKRNARVLVGGADRIENLLPKR"
     gene            complement(2837..3550)
                     /locus_tag="VGB76_02575"
     CDS             complement(2837..3550)
                     /locus_tag="VGB76_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097815.1"
                     /translation="MHDCLQTQDNLIDLLFNELEGATRSRLLAEVAACSRCGAQYRSL
                     NETLDVCAEASAAAMPRESYWPQYHAALNRRLHEAAGEARAEQRSAPFWRRLFTTSIR
                     VPVPLAATAVLLLFAVSVLAFSLVARSASETVFVAAPAASQGEAAPQIKFIEVPVIQE
                     KIVTQTIYVPRRNSGNQSAEATTGARRTPARENLAGDRRQNAPAPEASAPPRANLSGF
                     KPAGEVNLRIIKGSQAVEQ"
     gene            complement(3567..4256)
                     /locus_tag="VGB76_02580"
     CDS             complement(3567..4256)
                     /locus_tag="VGB76_02580"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0097816.1"
                     /translation="MSIALTHFVRRAKTNLIEQSESELIGRAQAGDAEAFSTLARAYE
                     RRLFVLALHHTRDRHDAEDLSQEVWLKAFRAIKSFRGESSFYTWLRQIMINTFLNDRR
                     APMYGGAQVVELDAWEASRRDAAHGSGDSGGSPLPARPRGLEETVEQQFLVTRVLDAL
                     GELTAQQRLIFLLKHQEGMTYEEISASLGCSTGTTKKALFRAVLKLRQQLGVEVEAAQ
                     YEPPRPVRRSC"
     gene            4470..5765
                     /locus_tag="VGB76_02585"
     CDS             4470..5765
                     /locus_tag="VGB76_02585"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002772.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY0097817.1"
                     /translation="MPSGQKVLAVILGGGAGSRLYPLTHLRSKPAVPLGGKYRLVDIP
                     ISNCINSDVMQIFVLTQYNSASLNRHIARTYRFSGFSDGFVEILAAEQTPDSPQWFQG
                     TADAVRQVLPHIHDWGIDTLLILSGDHLYRMDYRKFIARHVETKADVTVSVVPFVREA
                     AEEFGLLKADPTGRILEFREKPKGAALEEMRVDTTAFGLSAEEAARRPFLASMGIYVF
                     DYKRLRELLAQDKTWVDFGREVIPAAIKDYNVQSYLFDDYWEDIGTISAFHSANLDMT
                     SLLPKFNFFDTEAPIYTRPRYLPPSKVHHCEISDSIIGDGSIINRARLVRSVVGLRSR
                     IEPGARIEDTYLMGADYYQSIEELRADEAAGLPLIGIGRDTIIRHAIIDKNARIGANV
                     RLVNEAGVKHFDNEKAGYYIRDGIIIVPKNATVKNGTVI"
BASE COUNT         1069 a         1752 c         1885 g         1122 t
ORIGIN      
        1 ttcggcgagg gattgggcga gcctcgtgga tcacacgctg ctcaagccgg aggcgagcga
       61 ggcggacatc gaacgtctgt gcggtgaggc gtcgcagttt cattttgcga gcgtgtgtgt
      121 gaacccgacg tgggtgcggg cgtcggcctg tcacctgcgc gggacgggcg tggtcgtctg
      181 cacggtcgtc gggtttccgc tcggcgcgac gctggcagac gtgaaggcgt atgagacgcg
      241 gcgcgcgatc tatgacgggg cgcgcgaggt ggacatggtg atcaacgtcg gcgcgttgaa
      301 gagtggcgac gattgcgcgg tcgagtatga catcaggcaa gtggtggagg cggcgcacga
      361 gcatggagtg ctggtgaagg tgatcatcga ggccgcgcta ttgacggacg aggagaaggt
      421 gcgcgcgtgt ctggcggcga agcgtgcggg cgcggatttc gtgaagactt cgacgggctt
      481 tgcgaagggc ggggcgacgg tggcggacgt ggctttgatg cgtcgcacgg tcgggcagga
      541 gatgggcgtg aaggctgcgg gcggcgtcaa agggctggac gatgcgcgtg cgatggtcga
      601 ggcgggcgcg acaaggctcg gcgcgagcgt cggcgtgaag atcgcgcagg aggcttcggg
      661 cgtgcgaacg gcgacgagca gcaacggcgg ctattgaacg tcgcccgtcg aagcgacagt
      721 aaaggtcgtc cgtcgaagcg acagcaatag aatcaaagca ttcaacgcag agaacgcaga
      781 gaacacagag aaacgcggag ggggcgcaga gagagttgaa tttctctgcg ccccctctct
      841 ctgtgttctc tgcgtctctg tggtgagtct tatttcttca ccgtgaacta tcgtcagaag
      901 cggaagccga tgcccgcgtt gaattggaag ttgtgtgtgg tcgcgctgtt agagaacgtg
      961 acgaggccgg ggaatggtgg ccgctcatag ccgctgtcgc ggatgatggt gtcgccgatg
     1021 tcgaagcgcg tgacgatgcg gcgcgacggg tagaactcga ccacgccgcc gaggtctgcc
     1081 gcgaaatgcg tgacgccctg agtgcggaag tgggggataa tgaaagactg gccgtctatg
     1141 atttcgttgg taaagccgtt cactctcagc acccttgtga aatgcacgag gccggggcgg
     1201 gctttggcga agatgccaaa cttgtcgaag cgttttccgg ccttcacgcc gaacaatccc
     1261 tgtactgcgc gcccgccggt ttcctcgctg gggaaggttg actcggtcgg gtaaaagttg
     1321 gtttcggcct cgaccgcgac gttatcgttg aggttgacgg tgaagcgacc gccgaagccg
     1381 ggtttggtgt cggaagggcg attggcgagc gtgagtgaag agaactgcgc gcccacttcg
     1441 aatcgcggcg tctcgatggt ctgcgcaagc gtcgcgccgg aggcgaaaaa tagacacaga
     1501 aggactgcga ctgccgcgca gccgggtttc ttgttcatcg aattgtctcc ttagtgaatg
     1561 gttgattcgc gtactgacga agcaggctac ggaagccttc cggtcaagcg aacttgaata
     1621 aatgagaact gagtttgcgc tcgcgcctgt gttcggggcg ggcgcggcag cgtttgggct
     1681 agagcgtcag cgtttcggca gaaggttctc gatgcggtct gcgccgccga cgaggacgcg
     1741 agcatttcgt ttcgccgcgt tgcgcttcgc tttgtcgttc gtgcgtgtct caaactcccc
     1801 gcgctgcccc tcgcgtcgca ggcgcgccag agggtcttgc agccactcgg gcgaagcggg
     1861 tttcccctcg cgcccctcca cgatctgctc cgccgaagcg aagtcggaga cgtatccggc
     1921 atcgtgcagc acccacaaat tccagttgcc gccgttgccc accacgggcg cgctcgaagt
     1981 ccattcgtaa acgtcctgac cgagcgtgaa agaaattttg ttgttctcga tgcgcccgac
     2041 cttttgaaaa tcgtagccct cgtgcggcat cagcgagacg atgaagcgtc cgcgattcgg
     2101 cacgtaaaac cagaggagcg cgcccgcaga cccgcgcgcg ggcttctgac cgccgccgac
     2161 gagttcgccg ttgatcgtca atttgtaatc gcgcatggcg agctctacgt tgccgagcgt
     2221 gaaatcgcgc agcggcacgc tccggcgcgg cggcacgctc cacagcttcg cgcggtcgaa
     2281 agagactttg acgacatcca cgatcttcac gcccgtctgc gcattgacga gcaggtcgag
     2341 cgcgaaggcg tcgccgtcgt caacgaactg cgcgtcgccg gaacgcggca gcgtggagac
     2401 gttcggcggc gcgcggttcg ccccggcggc gggctgtcgc gcgcgaagtt tctcctcaaa
     2461 ctcctgactc aacggtttaa ccgaaacttt gaactgcttc gtcgccgcga cgggttcgac
     2521 gatcaggtcg taaccaaaca cgaagtttcc ctccgcgtcc acgagcacgc gatggacgac
     2581 gtggttgtct gcgagcatgg cctggggcgt cagagccgct tgaatctggc gcaggttgac
     2641 gttcgtgccg ggcgtgccga gcggcgcggc ctcgacgcgg aacgcgatga agccctcgcc
     2701 gtgcagaggc atgacgaact ggcctgcgcg ctctgtcgcc gcctcccctt tttgcgcgag
     2761 cgcgtgcggc gcggcgacga tgccgcacag cagcattaag agcagcgcga ggctgcatga
     2821 agatgagagt ttcgctttac tgttcaactg cttggctccc cttgatgatg cgtaagttca
     2881 cttccccggc gggcttgaag ccggagaggt tcgcgcgcgg cggggcagac gcctccggcg
     2941 cgggcgcgtt ctgccgcctg tctcccgcga ggttctcgcg cgccggtgtg cgacgcgctc
     3001 ctgtcgtcgc ctccgcgctc tggttgccgg agttgcgacg cggcacatag atcgtctgcg
     3061 tgacgatctt ctcctgtatg acgggcactt cgataaactt gatttgcggc gcggcttcgc
     3121 cttgcgacgc ggcaggcgcg gcgacgaaga ccgtttcgga cgcggaacgc gcgacgagcg
     3181 agaacgcgag gacggagacg gcgaagagca aaagcactgc ggttgcagcg agcggcacgg
     3241 gcacgcggat cgaggtcgta aacaggcgtc tccagaacgg cgcgctccgc tgctccgccc
     3301 gcgcttcgcc cgccgcttcg tgtagacggc ggttgagcgc ggcgtgatac tgaggccagt
     3361 agctttcgcg cggcatggct gcggctgacg cctcggcgca aacgtcgagc gtctcgttca
     3421 gcgagcgata ctgcgctccg caccggctgc aagccgcgac ttccgccagc aggcgtgaac
     3481 gcgtcgcgcc ctccagttcg ttgaagagca gatcgatcaa gttatcttgt gtctggagac
     3541 aatcgtgcat caaatttaac tcccgcttaa caacttctcc gcaccgggcg cggcggctcg
     3601 tactgcgccg cttcgacttc cacgccgagt tgctggcgca gtttcaggac ggcgcgaaac
     3661 agagccttct tcgtcgtgcc cgtcgagcag cccaaactgg cggagatctc ttcgtaggtc
     3721 attccctcct ggtgcttgag cagaaagatc agacgctgct gcgcggttaa ctctccgagc
     3781 gcgtcgagca cgcgtgtcac gagaaactgt tgttcgaccg tctcttccag accgcgcggg
     3841 cgtgcgggca ggggactgcc gccgctgtcg ccgctgccat gcgccgcgtc gcgccgcgag
     3901 gcttcccatg cgtcgagttc gacgacctgc gcgccgccgt acatgggcgc gcgccggtcg
     3961 ttgaggaacg tgttgatcat gatctgccgc agccacgtgt agaaactcga ctccccgcga
     4021 aaacttttga tcgcgcggaa agccttcaac cagacctcct gcgagaggtc ttcggcgtcg
     4081 tggcggtcgc gcgtgtgatg cagtgcgagc acgaagaggc gtcgctcgta ggcgcgtgcg
     4141 agcgtggaga aggcttctgc gtcgcccgcc tgcgcccgcc cgatcaattc gctttcgctc
     4201 tgctcgatca aattcgtctt cgcccttctc acgaaatgcg tcagtgctat agacacgccg
     4261 cgcgaaattt ggttggcctg atcagcgcgc gcgggattga aattcaggat tcggattcaa
     4321 ggggcgcggc aacttttctc cggctttttg tctccgcgcc gggacatctt acgccctgtt
     4381 ctgcgcgttc cgtcttgaaa cgcacgccgc tttgcgtcta tgctgaacgc acacaagttc
     4441 ttccagttca agtcgaatgg tgaaaagtga tgccctccgg acagaaggtg ttggcggtga
     4501 tcctcggcgg cggcgcaggc tcgcgtctct atcctttgac gcacctgcgt tcaaagcccg
     4561 ccgtgccgct cggcggcaag tatcgcctcg tggatattcc gatctcgaat tgcatcaact
     4621 ccgacgtgat gcaaatcttc gtgctcacgc agtacaactc ggcttcgctc aaccgccaca
     4681 tcgcgcgcac ctatcgcttc tccggcttct ccgacggctt cgtggaaatt ctcgccgccg
     4741 aacagacgcc cgacagcccg cagtggtttc agggaacagc cgacgccgtg cgtcaggtct
     4801 tgccgcacat tcacgattgg ggcatcgaca cgctgctgat tctctccggc gatcatctct
     4861 accggatgga ctatcgcaag tttatcgcgc gccacgtcga gacgaaggcg gacgtgaccg
     4921 tctcggtcgt gcccttcgtg cgcgaggcgg cggaagagtt cgggctgctc aaggccgacc
     4981 cgacggggcg catccttgag tttcgcgaga agccgaaagg cgctgcgctc gaagagatgc
     5041 gcgtggacac caccgccttc ggcctctcgg cggaagaggc cgcgcgccgc cccttcctcg
     5101 cctcgatggg catctatgtc ttcgactaca aacggctgcg cgaactgctc gcgcaggata
     5161 agacgtgggt ggacttcggg cgcgaggtga tccctgccgc catcaaggac tacaacgtgc
     5221 agtcgtatct cttcgacgac tactgggaag acatcggaac catctcggcc tttcactccg
     5281 caaacctcga catgacatcg ctcttgccga agttcaattt cttcgacacg gaagcgccca
     5341 tctacacgcg cccgcgctac ctgccgccct cgaaagttca ccactgcgag atcagcgatt
     5401 cgatcatcgg cgacggctcg atcatcaacc gtgcgcgcct cgtgcgctcc gtcgtcggcc
     5461 tgcgcagccg catcgaaccc ggcgcgcgga tcgaagacac ctacctgatg ggcgcagact
     5521 actatcaatc aatcgaggaa ctgcgcgccg acgaagccgc cggactcccg ctcataggca
     5581 ttggccgcga caccatcatc cgccacgcca tcatcgacaa aaacgcgcgc atcggcgcaa
     5641 acgtccgtct cgtcaacgaa gccggagtca aacacttcga caacgaaaag gcgggctact
     5701 acatacgcga cggcatcatc atcgtcccga agaacgcgac ggtgaaaaac ggcacagtga
     5761 tttaaagtga tgagtaatga gtgatgggtg atgagttaaa gacaactgct ttaactcatc
     5821 acccatca
//
LOCUS       DASWNS010000075         4926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_185376, whole genome shotgun sequence.
ACCESSION   DASWNS010000075 DASWNS010000000
VERSION     DASWNS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185376"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VGB76_02590"
     CDS             <1..348
                     /locus_tag="VGB76_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097818.1"
                     /translation="MLDVYFAVRYLQLRDDVQDEGADRSTLRTLARLRDANSLDAAAY
                     HALAEGYAALRRLDHHLRLIAGRSSRLPAAADHALLKDLARAAGYESAAALLSELERQ
                     MKAIRTAYERITT"
     gene            complement(474..692)
                     /locus_tag="VGB76_02595"
     CDS             complement(474..692)
                     /locus_tag="VGB76_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097819.1"
                     /translation="MNDKLIDERRAGGCPRCHAADLRAWSELDADEREVVRRLPASAV
                     YSPEERAARHLWCVRCWHEEPGGARSEA"
     gene            812..3802
                     /locus_tag="VGB76_02600"
     CDS             812..3802
                     /locus_tag="VGB76_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY0097820.1"
                     /translation="MFHNSRFSSSFSCALLLLVVLFVLPCLTNAQAQRDEPRFDFYAR
                     GPYRENVPRPQTTTRFDVGDFHTNYATMERVIERIAAAAPDRVRVTDIGETNEHRMMH
                     LLVISAPENLQRLDQIKANVARLADPRATNAGEAARIAAEMPIISFMSYTIHGNESAS
                     FEAMMQVVYQLAASNEPATLDILKNSVVLVNVCSNPDGHERFVTWYNSFGMGNADPLA
                     GEHREPWSIWGRANHYRFDLNRDNLAATQIETRNLQRAYLDWNPQIFVDHHGQPSQYF
                     FPPAALPVNPNLPPAQTARWLTEFGRANAAQFDARNWDYYVRDVFDLFGPFFWDSWPA
                     LNGATGMTYETDGGGTKGYAWRRDDDTIVTLRSAIAKHFVASMTTLETAARNREARLK
                     DFYEFKRSALEEGRREAMRRIVVVPGRDPVRAADLADVLLRSGIEVSMASEPFRSANA
                     HAYASGASSSTAASGAARTFPAGSYIVELAQPQKRLAKALLEPDTELDPAFVRDQLAR
                     FARNQRRGGNAAKEDYGFYDVTAWSLPLVFGVEAYWTGDAPAVASVALRPEPPAAQSS
                     ALDIVRASGERLLSRRLGVSGRATVAYIIPYETNGAASLAYRLQRENYKLAVATRTLN
                     AGGRDWPRGTIVVRVSRNPDTLHEAIARLAREASVEVTAVNSGFIETGDTSIGSEAVI
                     SLKRPRIIVVADDPVSQTSYGAMWWTFERYGVDFTPMTIANLRRANLDQYNVIIMPDG
                     SAGGYMSALGASGVGTLRAWVQRGGTLVCIKGAAVFAALKDVNLTSSRLVGSDDDEQR
                     DQKTPEDDEAKPAPASSPTPATPENATAREQSATARNRRQAAQTATEQQREAQDRSEK
                     LEGAPPDLPPIASPSARPGRVPEAVPGAIFRATVDRSTPLTYGYEDASLPVLVDSAYF
                     FRPSKEGTNAIVFTGEAGQTLRLAGFIWPNNTERLLRGTAHVIEEPTGRGHVVLYAED
                     PNYRGFYRAATRLFFNSFLFQPTF"
     gene            3952..4434
                     /locus_tag="VGB76_02605"
     CDS             3952..4434
                     /locus_tag="VGB76_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097821.1"
                     /translation="MFQIKAAFEEKLEIKTTIERARAFFGDVGNFVNLMPGVESIREE
                     AGGVVRWLIRAEVPVIGAIRQAFAVEQTLNHPQRIEWSPAAQERSNFLRYAAQFEEQG
                     ARVLVRIVQRVELRRQKATELHMLAGLVGENRLSAEMQKGVSEMMRTFLQRARTQLEQ
                     "
     gene            complement(4648..>4926)
                     /locus_tag="VGB76_02610"
     CDS             complement(4648..>4926)
                     /locus_tag="VGB76_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097822.1"
                     /translation="RLAHSLEGGEIKRGLEVWFHKHRETHLACPPPGLPGERSGRGVS
                     SCRETSSLGAPLAAEAQRRQVETCANLKVRLFALSHGARTQKERREKV"
BASE COUNT          914 a         1715 c         1430 g          867 t
ORIGIN      
        1 atgctcgatg tctatttcgc cgtgcgctac ctgcaactgc gcgacgacgt gcaggacgaa
       61 ggcgcagatc gttcgacgct caggacgctc gcacgcctgc gcgacgcaaa ctcgctcgac
      121 gccgccgcct atcacgcgct cgccgaaggc tacgccgcgc tgcgcaggct cgatcatcac
      181 ctgcgcttga tcgccggtcg ctcgtcgcgc ctgcccgccg cagcagacca cgcccttctc
      241 aaagacctcg cccgcgctgc cggttacgaa tcagccgccg ccctcctgtc tgaactggaa
      301 cggcagatga aagcgattcg cacggcctac gaacggatca caacatgacg taacgcccgg
      361 caacgaagtc gagccgcacc ggactcggag cgcgttgcca aaaactctct gcctgttttc
      421 ccttgcgcct cttcctctgc gcttcttcct ctgcgtctct tcctttgcgc ctctcacgct
      481 tcgctacgcg cgccgcccgg ctcttcgtgc cagcagcgca cgcaccagag atgccgcgcc
      541 gcgcgttctt cgggcgaata aacggctgac gcgggcagtc gccgcacaac ctcgcgctcg
      601 tctgcgtcga gttcgctcca cgcgcgcaag tctgcggcgt ggcaacgcgg gcagcctccc
      661 gctctgcgtt cgtcgatcaa tttatcgttc atagtctcta cgcccggcga atcatttaaa
      721 gccgaacttt tatttgagtg tcaagactgt gacagggtaa tatcgtcgcc gtctttatcc
      781 aactctcgta aaacagaaag ctgcctgccg aatgtttcac aacagtcgtt tctcgtcctc
      841 cttctcgtgc gctctccttc tgctcgtcgt tctcttcgtc ctcccctgtc tcacgaacgc
      901 acaggcgcag cgcgatgagc cgcgtttcga cttctacgcg cgtggcccct atcgcgaaaa
      961 cgtgccgcgc ccgcaaacca cgacgcgctt cgacgtgggc gactttcaca ccaactacgc
     1021 gacgatggag cgcgtcatcg aacgcatcgc cgccgccgcg cccgaccgcg tgcgcgtgac
     1081 cgacatcggc gagacgaacg agcatcgcat gatgcacctg ctcgttatct ccgcgccgga
     1141 gaatttgcag cgtctcgatc agatcaaggc gaacgtggcg cgtctcgccg acccgcgcgc
     1201 gacgaacgca ggcgaagccg cacgcatcgc cgccgagatg ccgatcatct ctttcatgag
     1261 ctacacgatt cacggcaacg agtcggcgtc gttcgaggcg atgatgcagg tcgtctatca
     1321 actcgccgcg tcgaacgaac cggccacgct cgacatcctc aaaaactcgg tcgtgctcgt
     1381 caacgtctgc tcgaaccccg acggccacga gcgtttcgtg acgtggtaca actccttcgg
     1441 catgggcaac gccgacccgc tcgcgggcga gcaccgcgaa ccctggtcaa tctggggacg
     1501 cgccaatcat taccgcttcg atctcaaccg cgacaatctc gccgccacgc agatagagac
     1561 gcgcaacctc cagcgcgcct atctcgactg gaacccgcag atcttcgtag accatcacgg
     1621 gcaaccctcg cagtatttct tcccgcccgc cgcgctcccc gtcaacccga acctgccgcc
     1681 cgcgcagacg gcgcgctggc tcacggagtt tggccgcgcc aacgccgcgc agttcgacgc
     1741 gcgcaactgg gactactacg tgcgcgacgt gttcgatctc ttcggcccct tcttctggga
     1801 ctcctggcct gccctgaacg gcgcgaccgg catgacctac gagacggacg gcggcggcac
     1861 caaaggctac gcgtggcgac gcgacgacga caccatcgtc accctgcgtt cagccatcgc
     1921 caaacatttc gtcgcctcga tgacgacgct tgagacggcg gcgcgcaacc gcgaagcgcg
     1981 gctcaaagat ttctacgagt tcaaacgaag cgcgctcgaa gaggggcgac gcgaagcgat
     2041 gcggcgcatc gtcgtcgtgc cgggacgcga ccccgtgcgc gcggctgatc tggccgacgt
     2101 gctcctgcgt tcggggatcg aagtgagcat ggcgagcgaa ccgtttcgtt cggcgaacgc
     2161 gcacgcttac gcgagcggcg cgtcttcttc aaccgcagcg agcggcgcgg cgcgcacctt
     2221 tcccgcaggc tcatacatcg tcgaactcgc gcagccgcag aagcgtctcg ccaaggctct
     2281 gcttgagccg gacacggaac tcgacccggc gttcgtacgc gaccaactcg cgcgtttcgc
     2341 acgcaaccag cgacgcgggg gcaacgccgc gaaggaggat tacggctttt acgacgtgac
     2401 ggcgtggtct ttgccgctcg tcttcggcgt cgaagcctat tggacgggcg acgctccggc
     2461 ggtcgcttcc gtcgcgcttc gccctgagcc tcccgccgcg cagagttccg cgctcgacat
     2521 cgtgcgcgcc tccggcgaaa ggctgctctc gcgtcgcctc ggcgtctcag gtcgtgccac
     2581 ggttgcctac atcatccctt acgagacgaa cggcgcggcc tcgctcgcct atcgcctgca
     2641 acgcgaaaac tacaaactcg cagtcgccac gcgcaccctg aacgcgggcg ggcgcgattg
     2701 gccgcgcggc acaatcgtcg tgcgcgtctc gcgtaatccc gacacgctgc acgaagccat
     2761 cgcgcggctc gcacgcgaag cgagcgtcga agtcaccgcc gtcaattccg gcttcatcga
     2821 gacgggcgac acgagcatcg gctcggaagc ggtcatctcc ctgaaacgac cgcgcatcat
     2881 cgtcgtcgcc gacgaccccg tgagccagac cagctacggc gcgatgtggt ggaccttcga
     2941 acgctacggc gtagacttca cgcccatgac catcgccaat ctccggcgcg caaacctcga
     3001 ccagtacaac gtgatcatca tgcccgacgg ctctgcgggc ggctacatga gcgcgctcgg
     3061 cgcgtcgggc gtcggcacgc tgcgcgcgtg ggtgcagcgc ggcggcacgc tcgtctgcat
     3121 caaaggcgcg gcggtcttcg cggctttgaa ggatgtcaac ttaacttcct cgcgccttgt
     3181 cggcagcgac gacgacgagc agcgcgatca aaagacgcca gaagacgacg aagccaagcc
     3241 cgcgcccgcc tccagcccca cgcccgccac gccggaaaac gcgacggcgc gcgagcaatc
     3301 ggccacggcg cgcaaccgca ggcaggcggc gcagaccgcc accgaacagc aacgcgaagc
     3361 gcaggaccgc agcgaaaaac tcgaaggcgc gccgcccgac ctgccgccca tcgcgtcgcc
     3421 ctcggcgcgt ccgggtcgcg tgcccgaagc cgtccccggc gcgatcttcc gcgccaccgt
     3481 agaccgctcc acgccgctca cctacggcta cgaagacgcg tcgctccccg tgctcgttga
     3541 ctcggcctac ttcttccgcc cgtcgaaaga gggcacgaac gccatcgtct tcacgggcga
     3601 ggcggggcag acgttgcgcc tcgcagggtt catctggcct aacaacacgg agcggctcct
     3661 gcgcggcacg gcgcacgtca tcgaagaacc gacggggcgc ggccacgtcg tcctctacgc
     3721 cgaagacccg aactatcgcg gcttctatcg cgccgccacg cgcctcttct tcaattcgtt
     3781 tctctttcaa cccacgtttt gagagcagtg acaagtgacg agtgaccagt gacgaggttc
     3841 ctacagtgac aggcgacggg tgacaagtta gaatcttaaa ggataggtga cgggttgaag
     3901 atcggctcta actcgtcact tatcactcgt cacttgtcat tgggagatat tgtgtttcag
     3961 atcaaagcgg ctttcgaaga aaaacttgag attaagacga cgattgagcg tgcgcgcgcc
     4021 ttctttggcg acgtgggcaa tttcgtgaat ttgatgccgg gcgtggagag catacgcgag
     4081 gaggccgggg gcgtggtgcg ctggctgatc cgcgcggaag tgccggtcat cggcgcgatc
     4141 cggcaggcgt tcgccgtcga gcagacgctc aatcacccgc agcgcatcga gtggtcgccc
     4201 gccgcgcagg agcgcagcaa ttttctgcgc tacgccgcgc agttcgagga gcagggagcg
     4261 cgcgtccttg tacgcatcgt gcagcgcgtc gagttgcgca ggcagaaagc gacggagttg
     4321 cacatgctcg ccggactcgt cggcgaaaac cggctcagcg cggagatgca gaagggcgtc
     4381 agcgaaatga tgcgcacttt cctccagcgc gcacgcacgc aactggaaca gtagcctgtc
     4441 gcccgtggcc ggtggttcaa tccaaatctc cggcagtctg tgtgatagcc acaggacacg
     4501 gcaaccggtt gccggtcgct ccccgcgatt tatcttcttg caaggacaca gattatcaag
     4561 taatctggaa agtggttgac cttcccacgg cgggacttgc ccacatcttc cggtcaacct
     4621 cctcagccaa cgccttagct ctttagttca cactttctcg cgccgctctt tttgcgtgcg
     4681 cgcaccgtgc gacaaagcaa ataacctgac tttgagattg gcgcaagtct ccacctgcct
     4741 gcgctgagct tctgctgcga gcggcgcgcc caaactcgaa gtctcccgac aagacgagac
     4801 tcctctccct gacctctcgc ccggcagccc cggcggcgga caagcgagat gcgtctcacg
     4861 atgcttatga aaccaaacct ccagcccgcg tttgatttcg ccgccttcca gagagtgtgc
     4921 gagacg
//
LOCUS       DASWNS010000076         8681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_132606, whole genome shotgun sequence.
ACCESSION   DASWNS010000076 DASWNS010000000
VERSION     DASWNS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132606"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VGB76_02615"
     CDS             <1..625
                     /locus_tag="VGB76_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097823.1"
                     /translation="PELVSGGRAYVHRMLNEAAARLAQHGEVISPEIIDAAERLCIVE
                     HTDHKRFKTEDHASLYNEIFTLYALNVGDTYRFSVSSAGAGGMVQMIPPTYKSIREMY
                     PSVGLNPDFVAGMRDHSNALEAMLLYMQSTWDDLLEREQVRHALAANIATQTELLAAG
                     YNSNPTRLHLYLERGGTNWRALIPAETQMYLRIYASVDRLVEFKERA"
     gene            825..1121
                     /locus_tag="VGB76_02620"
     CDS             825..1121
                     /locus_tag="VGB76_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097824.1"
                     /translation="MEINDEAKELVQEIGEAINAAVERSPQVADAISRLRAAGYEMEL
                     TLKLEIGLRETGIEEDTEVSASANGDGAQDATLELTDEDRRTLRSMKIRFDESE"
     gene            1266..2792
                     /gene="atpD"
                     /locus_tag="VGB76_02625"
     CDS             1266..2792
                     /gene="atpD"
                     /locus_tag="VGB76_02625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY0097825.1"
                     /translation="MSTPNGNAGNGHNIGRVVQIIGPVLDVEFEENHLPPIYTALRVT
                     SEGFDVPYPIDIICEVEQHLGEGRVRTVSMKPTEGVVRGMRALDTGGPITVPVGNMTL
                     GRIMNVIGEPVDTLGAVNSETRYPIHRHAPTLDEQSTELEMFETGIKVIDLIQPFLRG
                     GKIGLFGGAGVGKTVLIMELINNVAKAHSGFSVFAGVGERTREGNDLLREMVESKVIN
                     FGEAFYQHMEETGEFDLTKVDLKAVEESKVALVYGQMTEPPGARLRVALSGLTVAEYF
                     RDQGLDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPNLATEMGEMQERITSTKTG
                     AVTSIQAVYVPADDYTDPAPATTFAHLDAVTALSRQIAELGIYPAVDPLSSSSRLLDP
                     RIIGEEHYRTAQDVKRILQRYKELQDIIAILGLDELSEEDKLAVSRARKVQRFFSQPF
                     FVAAQFTGLEGKYVKLEETIRGFREIIDGNADDLPEQAFFLQGTIEDVRARARQIAES
                     "
     gene            2885..3301
                     /locus_tag="VGB76_02630"
     CDS             2885..3301
                     /locus_tag="VGB76_02630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY0097826.1"
                     /translation="MADRLQLDIITPERGLLSEAVDAVTLPGLGGELGILPGHTPLIS
                     QLQTGVLSYTQGGATKRLLVSGGFVEVSDDRVAVLAELAERPEEIDAARARSERDQAE
                     KLLGAATGSEDDLSRARTQFERASARLQIAGDSSSH"
     gene            3472..4524
                     /gene="ruvB"
                     /locus_tag="VGB76_02635"
     CDS             3472..4524
                     /gene="ruvB"
                     /locus_tag="VGB76_02635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY0097827.1"
                     /translation="MPQESTLETVAEAREISGEATPEDRQVEPSLRPRSLEEYIGQTK
                     ITTNLKIFLRAALSRREALDHVLFFGPPGLGKTTLSQIIAQEMNAPLKTVSAPAIERG
                     GDLAAVLTNLQEGEVLFIDEIHRLKPQIEEVLYSAMEDYQLDIMIGQGTAARSIKLDL
                     PKFTLVGATTRAGLITAPLRGRFGIVFHLDFYSQKDLEIIVHRSAEILNVEIEATGAK
                     EIARRARGTPRIANRLLRRVRDYAQVLGDGRITQALSKRALDEMEVDKYGLDEVDRKI
                     LTAIIEKFDGGPVGIGTIAASISEERESIEEIIEPYLIQIGFLNRTPRGRVATRAAYK
                     HLGIALPKIQQDLLAS"
     gene            4580..4810
                     /locus_tag="VGB76_02640"
     CDS             4580..4810
                     /locus_tag="VGB76_02640"
                     /inference="COORDINATES: protein motif:HMM:NF022716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEY0097828.1"
                     /translation="MTDKQTQEQAVESNTIKDPDNWTTGDEPMTGAQRSYLTTLSEEA
                     KEEFDENLTKAEASKRIEELQQKTGRGLEQKS"
     gene            complement(4996..5745)
                     /locus_tag="VGB76_02645"
     CDS             complement(4996..5745)
                     /locus_tag="VGB76_02645"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY0097829.1"
                     /translation="MTARLNVNIDHVATVRQARRAPEPSLVAAAMICEGAGADGITIH
                     LRADRRHIQDEDLHTLRRVVTTYLNLELSVSEEMTRIALDTQPDAVSLVPENPNEITT
                     EGGLDVTTQLETVRGAIDRLREGGIFASIFLDPEPAQITAAHEAGAQQVELCTALYAE
                     ATLGARGFHGEGAARVAHELQRLHEAATLAAQYGLRVAAGHGLTYRNVGAVARIQEIT
                     EFNIGHNIISRAVLVGLERAVREMREEIARS"
     gene            complement(5830..6687)
                     /locus_tag="VGB76_02650"
     CDS             complement(5830..6687)
                     /locus_tag="VGB76_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEY0097830.1"
                     /translation="MSLNLSFVIPAYNEAARLGKTLQTAFEYLNAQTSESEIIVVDDG
                     STDETTQIAEQSFEKFSGRTGSRLIRVAPNRGKGYAVRAGLLAAHAPIALFSDADLST
                     PLTETPKLVAPIRAGEYDLVFGSRALDRSLIGVHQPWRREQSGRVFNLVMRLATGLPF
                     WDTQCGFKAFRLDVCRPIIENSKIDRFGFDVELLYVAHLAGLRLHERAVRWNDVAGSK
                     VSFMNGLDGFLELRELRREARRGSYDEAIRAARIVAESREGRDGLFNEHDAPESDVSR
                     ELASASRRI"
     gene            6912..8642
                     /locus_tag="VGB76_02655"
     CDS             6912..8642
                     /locus_tag="VGB76_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0097831.1"
                     /translation="MKKRLGLIIAALVVVVASILGGVALRRVQQRIVTGTSADADAII
                     EHDYREVLSVISAEYADEIDYEKANQAAIQGMLSTLDPHSTFFTRADYDKLKQDHDSE
                     FYGIGVTILRHRDGVYVQSPVPNTPAARAGLRYGDRIVEVDGKDAREWTSEGVSKAVR
                     GERGEPVKLKVERAGALAPVYVTIFRDAVSQPSVRTAYMIRPGTGYVALTGGFTNTTS
                     DELEAAIKRLKEEGLRQLVLDLRNNPGGLLKESINVASQFLPRGQTIVSVRGRGEYGT
                     PQTFENTAYDPEDFPLVVLVNRNSASASEIVAGAIQDYGRGYLVGETTFGKGLVQRVF
                     SLDYGTGLTLTTQKYYTPYGRSIQRDYSSGSFYDYYVRHDPEDEPQPSTPRAPTLAAT
                     PNAHSTIVEPTPTPAPTPHPPTGPAVKTAAGRVFYGGGGITPDIEVKPLDVSSPVRVR
                     IFEAAFQFTRELVAGQIPGLESYRVEETRYGQFPRPTDLPVNERVLEAFRAHALRDAE
                     SGLTNAQIDAELDYVRLRVREEIVTAAYGNEAAIRALLDSDPQLLRALEVFPDAKRLA
                     EMVRSGASQS"
BASE COUNT         1800 a         2736 c         2625 g         1520 t
ORIGIN      
        1 gcccgaactc gtgagcggcg gcagggctta cgtccatcgt atgctgaacg aggccgccgc
       61 gcgtctggcg cagcacggcg aagtgatctc gcccgagatc attgacgccg ccgagcggct
      121 ctgcatcgtc gagcacacgg atcacaagcg tttcaagacc gaagatcacg cctcgctcta
      181 caacgaaatt ttcacgctct acgcgctgaa cgtgggcgac acctatcgtt tctccgtcag
      241 ttcggcgggc gcgggcggca tggtgcagat gatcccgccg acctacaagt cgatcaggga
      301 gatgtaccct tccgtcggac tcaatcccga cttcgtcgcg ggcatgcgcg accactcgaa
      361 cgccctcgaa gccatgctcc tctacatgca gagcacctgg gacgacctgc tggaacgcga
      421 acaggtcagg cacgcgctcg ccgcgaatat tgccacgcag accgaactgc tggccgccgg
      481 ttacaactcg aaccccacgc gcctccacct ctatctcgaa cgcggcggca caaactggcg
      541 cgcgctcatc cccgccgaga cgcagatgta cctgcgcatc tatgcttcgg ttgaccgtct
      601 cgtcgagttc aaggagcgcg cctagaacat ttccgccgcc gcagaccgac gcccgacggg
      661 aggcgcgcga aacgtgcgga caatccggca aaattaaaga gcgcggagcg accaactccg
      721 cgctctttgc ctgtcttccg gttgaccaaa ggcgcgtcgt aagcgtagca tcgcgggcaa
      781 ctatcagaca gccaaacgat gagccgggtc gcgggagaga cgagttggag atcaacgacg
      841 aagcaaaaga gttggtgcag gagatcggcg aagcgatcaa cgcggcggtg gagcgttcgc
      901 cgcaggtggc cgacgccatt tcccgcctgc gcgcggcggg ctacgagatg gaactcacac
      961 tgaaattgga gatcggtttg cgcgaaaccg gaatcgaaga ggacacggaa gtctctgcgt
     1021 cggccaacgg agacggagcg caggatgcga cgctcgaact cacggacgaa gaccgccgca
     1081 cgctccgcag catgaagatt cgcttcgacg aaagcgaata gacgcgcctc acgcctcagg
     1141 ctcgaacagt cgcccaacta aacgcgcttt gccttagttt gcgcggttat gctatcttca
     1201 accccttaag taatccgcta atttttcgcc tttaacaaga cacacagagg agttgcgcta
     1261 aagttatgtc tacgcctaat ggaaacgccg ggaacggcca taatataggc agggtggtgc
     1321 agatcatcgg tcccgtgctc gacgtggaat ttgaagagaa ccacctgccg ccgatctaca
     1381 ccgcgttgcg cgtcacgtcc gaaggcttcg acgtgccata cccgattgac atcatctgcg
     1441 aagtcgaaca gcacctcggc gaaggccgcg tccgcaccgt ctcgatgaag ccgaccgagg
     1501 gcgtcgtgcg cggcatgcgc gccctcgaca ccggcggacc aatcaccgtc cccgtcggca
     1561 acatgaccct cgggcgcatc atgaacgtca tcggcgaacc cgtggacacg ctcggcgcgg
     1621 tcaactccga gacgcgctac cccattcatc gccacgcgcc cacgctcgac gagcaatcga
     1681 ccgaactcga aatgttcgaa acgggcatca aggtgatcga cctcatccag ccgtttttgc
     1741 gcggcggcaa gatcggcctc ttcggcggcg cgggcgttgg caagaccgtc ttgatcatgg
     1801 aattgatcaa caacgtcgcc aaggctcact ccggcttctc cgtgtttgcg ggcgtcggcg
     1861 agcgcacgcg cgaaggcaac gacctcctgc gcgagatggt cgaatcgaaa gtcatcaact
     1921 tcggcgaagc cttctatcaa cacatggaag agacgggcga gttcgacctg acgaaggttg
     1981 atctcaaggc ggtcgaagag tcgaaggtcg cgctcgtcta cgggcagatg accgaaccgc
     2041 cgggagcgcg cctgcgcgtc gcactctccg gcctgaccgt cgccgaatac ttccgcgatc
     2101 agggcttgga cgtgctgctc ttcatcgaca acatcttccg cttcacgcag gcgggttcgg
     2161 aagtgtccgc gctgctcggg cgcatgcctt cggcggtcgg ttatcaaccg aacctcgcca
     2221 ccgagatggg cgagatgcag gagcgcatca cttcgacgaa gaccggcgcg gtcacgtcca
     2281 ttcaggccgt ctacgttccg gcggacgact acacagaccc cgcgcccgcc acgaccttcg
     2341 cgcacctcga cgccgtcacc gcgctctcgc gccagatcgc cgaactcggc atctacccgg
     2401 cggtcgatcc gctctcgtcc tcttcgcgcc tgctcgaccc gcgcatcatc ggcgaagagc
     2461 actaccgcac ggcgcaggac gtgaagcgca tcctgcaacg ctacaaggaa ctgcaagaca
     2521 tcatcgccat tctcggcctc gacgaactct cggaagaaga caagctcgcc gtctcgcgtg
     2581 cgcgcaaagt gcaacgcttc ttctcgcagc ccttcttcgt cgccgcgcag ttcaccggtc
     2641 ttgaaggcaa gtacgtcaaa ctcgaagaaa ccatcagagg cttccgcgag atcatcgacg
     2701 gcaacgccga cgatctaccc gaacaagcct tcttccttca aggcaccatc gaagacgttc
     2761 gcgcccgcgc gcgccagatc gcagaatcgt agaacaaaag tgatgagtaa tgagtgatgg
     2821 gtgatgagtt aaagacagac tcttctcact catcgctcat cacccatcac tcatcactat
     2881 aatcatggct gacagattac aactcgacat catcacgccg gagcgcggcc tgttgtcgga
     2941 agcggttgac gccgtgacgc tgccgggctt gggcggcgaa ctcggcatcc tgcccggcca
     3001 cacgccgctc atctcgcaac tgcaaaccgg cgtcctctct tacacgcagg gcggcgcgac
     3061 caaacgcctg ctcgtctccg gcggcttcgt cgaagtgagc gacgaccgcg tggccgtgct
     3121 cgccgaactc gccgaacgcc ccgaagagat cgacgccgcg cgcgcccgct ccgaacgcga
     3181 tcaggccgag aaattactgg gcgcagccac cggctcggaa gatgatctct cgcgcgcccg
     3241 cacgcagttc gaacgcgcca gcgcgcgcct ccagatcgcg ggcgatagtt cgtcgcacta
     3301 agctaaaact ccaaatttac aggcaggagc ggaagttagc ggtcatccgt caacttccgt
     3361 tcctgctttt tatggcaaac tatttttgtt tcatgcttgc aacaacgatt caaaagcggt
     3421 ataatccctc ttgtctgaaa cacggggaaa gaagaccagg ggagagagtt gatgccacag
     3481 gaatccacgc ttgagacggt tgcggaggcg cgcgaaattt cgggcgaggc aacgccggaa
     3541 gatcgtcagg tcgaaccgag tttgcgcccg cgctcgctcg aagagtatat cggccagacc
     3601 aagatcacga ccaatctgaa gatctttttg cgcgccgcgc tatcacgacg cgaagcgctc
     3661 gatcacgtcc tcttcttcgg cccgccggga ctcggcaaga cgactctttc gcagatcatc
     3721 gcgcaggaga tgaacgcgcc tttaaagacc gtgagcgcgc ccgccatcga acgcggcggc
     3781 gacctcgcgg ctgtcctgac caacttgcag gaaggtgaag tgctcttcat agacgagatt
     3841 catcgtctca agccgcagat cgaagaggtg ctctactcgg cgatggagga ttatcaactc
     3901 gacatcatga tcgggcaggg cacggcggcg cgctccatca aactggactt accgaaattc
     3961 accttagtcg gcgcgacgac gcgcgccgga ctcatcaccg cccccttgcg cggtcgcttc
     4021 ggcatcgtct ttcatctcga cttctactcg cagaaagacc ttgagatcat cgtccaccgc
     4081 tcggctgaaa tcctgaacgt ggagatcgag gcgacgggcg cgaaagagat cgcgcggcgc
     4141 gcacgcggga caccgcgcat cgccaaccgc ctgctcaggc gcgtgcgcga ctacgcgcag
     4201 gtcttgggcg acgggcgcat cacgcaggcg ttgtcaaagc gcgcgctcga cgagatggaa
     4261 gttgacaagt acgggctgga cgaagtggat cgcaagattc tgacggcgat catagagaag
     4321 ttcgatggcg gtcccgtcgg catcggaaca atcgccgctt cgatcagcga ggagcgcgag
     4381 tccatcgaag agatcatcga gccttatttg atccagatcg gattcctcaa tcgcacgccg
     4441 cgcgggcggg tcgccacgcg cgcagcctac aagcatctcg gcatcgcgct gccgaagata
     4501 cagcaggatt tgctggcgag ttaatttcag cgagttaagt tcgaaggaac atcaaacgaa
     4561 ccgaaggggg aacgggaaag tgacagacaa gcagacgcag gaacaggcag ttgagagcaa
     4621 caccatcaaa gaccctgata actggacgac gggcgacgag ccgatgaccg gcgcgcagcg
     4681 ttcgtacctg acgacgcttt cggaagaggc gaaagaagag ttcgacgaga acctgacgaa
     4741 ggccgaagcg tcgaagcgca tcgaagagtt gcagcaaaag acgggtcgcg ggttggaaca
     4801 gaagtcgtaa gttgcgccgt cgcctgcggc gcggtgaggc aacaatttct acgagtttcg
     4861 gtcagtttcg gctggccgaa ggtaacgccg gggagcatcg gttggggagc ggatcgggag
     4921 ccactcttcg acaggacagc tctccggcgt ttttatgcgc gcaagcgtgc gcggaagatt
     4981 cgagcgaaag ccggactaac tccgtgcgat ctcttcgcgc atctcgcgca cggctcgttc
     5041 gaggccgacg agaacggcgc gggagatgat gttgtggccg atgttgaact ccgtgatctc
     5101 ctgtatgcgc gccactgcgc cgacgtttcg ataggtgagt ccgtgcccgg cggcgacgcg
     5161 caggccgtat tgcgcggcga gcgtggcggc ttcgtggagg cgttggagtt cgtgtgcgac
     5221 gcgcgccgcg ccttcgccgt ggaagccgcg tgcgccgaga gtcgcttccg cgtagagcgc
     5281 ggtgcagagt tccacttgct gcgcgcccgc ttcgtgcgcg gcggtgatct gcgctggctc
     5341 cgggtcgaga aagatgctgg cgaagattcc gccttcgcgt aagcggtcaa tcgcgccgcg
     5401 cacggtttcg agttgcgtgg ttacgtcgag gccgccttcg gtcgtgatct cgttcgggtt
     5461 ttccgggacg agcgagacgg cgtcgggctg cgtatcgagc gcgatccggg tcatctcttc
     5521 ggagacgctc aattcgaggt tgagataggt cgtgacgacg cggcgcagcg tgtggaggtc
     5581 ttcgtcctga atatgcctgc ggtcggcgcg caggtggatg gtgatgccgt cggctccggc
     5641 tccctcgcag atcatggccg ccgccacgag gctcggttcg ggcgcacgcc gcgcctgcct
     5701 gacggtcgcc acatgatcga tattaacgtt gagtcgcgcg gtcatagacg cctccgtgga
     5761 aacagaaata gtaacgccgc gacttcgaga gttcgggcac ggcgtaacga tcacttcgca
     5821 tgcaaaagat caaatgcggc gcgacgcgct ggcgagttcg cgtgagacat cgctttcggg
     5881 cgcgtcgtgt tcgttgaaca ggccgtcgcg cccttcccgg ctctcggcga cgatgcgtgc
     5941 ggcgcggatc gcttcgtcat aactgccgcg cctggcttcg cggcgcagtt cgcgcagttc
     6001 gaggaagccg tcgaggccgt tcataaaact gaccttgctg cccgcgacat cgttccagcg
     6061 cacggcgcgt tcgtgaagtc gcaagcccgc cagatgcgcg acgtagagca gctccacgtc
     6121 gaagccgaag cgatcaattt tagagttttc gatgatggga cggcacacgt cgaggcggaa
     6181 ggctttaaag ccgcactgcg tatcccagaa gggcagtccg gtggcgagcc gcatcacgag
     6241 gttgaagacg cgcccgctct gctcacgccg ccagggctgg tgcacgccga tgagcgaacg
     6301 gtccagcgcg cgcgaaccga agacgagatc atattcgccc gcacgaatcg gcgcgacgag
     6361 tttcggcgtc tccgtgagcg gcgtggagag gtctgcgtcg gaaaagagcg cgatgggggc
     6421 gtgcgcggcg agaagtccgg cgcgcacggc gtagcctttg ccgcgattgg gggcgacgcg
     6481 gatgagacgg ctgccggtgc ggcctgaaaa cttctcgaaa ctctgctcgg cgatctgcgt
     6541 cgtctcgtcc gtcgagccgt cgtcaacgac gatgatctcg ctctcggaag tttgcgcgtt
     6601 caaatactcg aaggccgtct gcaaagtttt acccagacgc gccgcttcgt tataggccgg
     6661 gatgacgaat gaaagattga gcgacatgaa aactcccaaa ggatgacgag tgacagcgac
     6721 aagtgatgag tgacatgaca aaacaagagt ctatctgctt gccggtcgcc caccaatagc
     6781 catttctttt atagcacagg ccatttgcgg tgcgcgcggt ttcggcagcg tgcgggcggc
     6841 tgtgttaaaa ttttcattga tgaatgcgtt ggttgtgggc agaggatttt gacggaagct
     6901 gtgaagaagt tatgaagaag agattgggtc tgatcattgc cgctctggtc gtcgtcgtgg
     6961 cgagcatcct gggcggcgtc gcgctgcggc gtgtgcagca gcggatcgtg acgggcacga
     7021 gcgcggacgc cgacgccatc atcgaacacg attacagaga ggtgctgtcg gtcatctctg
     7081 cggaatacgc ggacgagatc gactatgaga aggccaacca ggccgccata cagggcatgc
     7141 tctccacgct cgacccgcac tccaccttct tcacgcgcgc cgactacgac aagctgaaac
     7201 aggatcacga ctccgagttt tacggcatcg gcgtgaccat cctgcgccac cgcgacggcg
     7261 tctacgtgca gtcgcccgtg ccgaacacgc ccgcggcgcg cgccggtctg cgttacggcg
     7321 accgcatcgt cgaagttgac ggcaaggacg cgcgcgaatg gacgagcgaa ggagtttcca
     7381 aggccgtgcg cggcgaacgc ggcgagccgg tcaagctcaa agtcgaacgc gcgggcgcgc
     7441 tcgctcccgt ctatgtcacc atcttccgcg atgccgtctc gcagccctcc gtacgcaccg
     7501 cctacatgat tcgccccggc accggctacg tcgctttaac cggcggcttc accaacacca
     7561 cgagcgacga actggaagcc gccatcaaac ggctcaagga agagggcttg cgccaactcg
     7621 tcctcgacct gcgcaacaac cccggcgggc tgctcaagga atcgatcaac gtggcgagcc
     7681 aatttttgcc gcgcgggcag accatcgtga gcgtgcgcgg gcgcggcgaa tacggcacgc
     7741 cgcaaacttt tgaaaacacc gcctacgacc cggaagactt tccgctcgtc gtgctcgtca
     7801 atcgcaactc ggcttcggct tccgagatcg tggcgggtgc gatacaggat tacgggcgcg
     7861 gctacctcgt cggcgagacg accttcggca agggcttggt gcaacgcgtc ttctcgctcg
     7921 attacggcac gggtctgacg ctcaccacgc aaaaatatta cacgccctac ggtcgctcga
     7981 ttcaacgcga ctattcgagc ggctcgttct acgattacta cgttcgccac gacccggaag
     8041 acgaaccgca accgagcacg ccgcgcgcgc ccacgctcgc cgcgacgccg aacgcacatt
     8101 ccactatcgt cgaaccaacg cccacgccgg ctccaacgcc gcacccgccc acaggtcctg
     8161 ccgtgaagac cgcagccgga cgcgtcttct acggcggcgg cggcatcacg cccgacatcg
     8221 aagtgaaacc gctcgacgtg agcagccccg tgcgcgtgcg tatcttcgaa gccgcttttc
     8281 agttcacgcg cgaactcgtc gccggtcaaa ttcccggcct cgaaagctac cgcgtggaag
     8341 agactcgcta cggccagttt ccccgcccca ccgacctccc cgtcaacgaa cgcgtcctcg
     8401 aagcctttcg cgcacacgcc ctgcgcgatg ccgagtcggg tttgacgaac gcgcagattg
     8461 acgccgaact cgactacgtg cgcctgcgcg tccgcgaaga gatcgtcacc gccgcttacg
     8521 gcaacgaagc cgccatccgc gccctgctcg acagcgaccc gcaactcctg cgcgccctcg
     8581 aagtcttccc cgatgccaaa cggctcgccg aaatggtgcg cagcggcgca tcgcaaagtt
     8641 aacaaggcaa aattcagttc gccgcgcccg tccgacgcaa c
//
LOCUS       DASWNS010000077         3700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_496214, whole genome shotgun sequence.
ACCESSION   DASWNS010000077 DASWNS010000000
VERSION     DASWNS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496214"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VGB76_02660"
     CDS             <1..407
                     /locus_tag="VGB76_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0097832.1"
                     /translation="GAAEVIASAFIERKRGEVVGEKSFGAGAEQQLFPLRNGDGLLLT
                     TVKWASSAGKSFIGAGVTPTVEVKRPELAAAIDPDELTGNDDDAIAAPAPPQNGREVA
                     PEATSPKQQPEDTQLKKALELLNKPATQQKAA"
     gene            complement(521..2095)
                     /locus_tag="VGB76_02665"
     CDS             complement(521..2095)
                     /locus_tag="VGB76_02665"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY0097833.1"
                     /translation="MASTRTETKPALSKIERLRERERRAEEGGGRERIERQHAAGKLT
                     ARERIAFLLDEGTFEEFDKHVVHRSRDFGLGEQLYPGDGVVTGYGLVDNRRVFVFAQD
                     FTVFGGSLSETHAEKICKVMDLAMRVGAPVIGLNDSGGARIQEGVLSLGGYADIFLRN
                     TLASGVVPQISCILGPCAGGAVYSPAITDFNVMVKNTSYMFITGPEVIRAVTHEEVSK
                     EELGGADTHNRISGVAHFDADSDEHALQLVRELLSFLPSNNMDEAPRVPTADPADRRD
                     ERLNSIVPEASNQPYDIRDVIHAVIDENYFFEVHEFYAPNIVVGFARLDGRSVGIVAN
                     QPAYLAGVLDIDASVKAARFVRFCDCFNIPLVVFEDVPGFLPGVRQEHGGIIRHGAKL
                     LYAFAEATVPKITVITRKAYGGAYCVMASKHIRTDINLAWPTAEIAVMGAGGAVGVLY
                     RREIGEAADPESARSARITEFEEKFANPYVAAERGFIDEIIEPAETRRKLIRALALLE
                     NKRDTNPPKKHGNIPL"
     gene            complement(2166..2690)
                     /locus_tag="VGB76_02670"
     CDS             complement(2166..2690)
                     /locus_tag="VGB76_02670"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEY0097834.1"
                     /translation="MGVESSAAGAEGAQARPPVTVHARKFDGRLHREWQAGLVRRVGS
                     LVVVEGVFAEEIRHDQLGTIAPGTLSAEYYWTDRWYSVFRFHEPWGALRNYYCNINQP
                     AKFDGHVLTFTDLDIDVLVAPDFSYRILDEEEFAANAALYGYPESVRESARHALAELL
                     SLIERREHPFDGQM"
     gene            complement(2709..3497)
                     /locus_tag="VGB76_02675"
     CDS             complement(2709..3497)
                     /locus_tag="VGB76_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0097835.1"
                     /translation="MSKKICRFLNPNEGEASYGLIEDETVYPVSAAQIFASSHAPARA
                     DAGFALSEVKLLAPVAPSKIVCVGRNYKEHAAELGNPMPDEPLLFLKPPSSVVGHQDA
                     IELPPVSSRVEHEGELGVVIGRRAHRLSDSENPLHYVLGYTCVNDVTARDLQRRDVQF
                     TRAKSFDTFCPVGAYIVCGLDPFDLGVETRVNGEVRQSGRTSAMAFPVSYLIRYISHI
                     MTLEPGDLISTGTPAGVAPLADGDVCEVEVEHVGLLANPVRAGR"
     gene            complement(3498..>3700)
                     /locus_tag="VGB76_02680"
     CDS             complement(3498..>3700)
                     /locus_tag="VGB76_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097836.1"
                     /translation="GYNVYRSTDPSAPKERWTKLNRTLLERTTFQDDAVESGTKYFYY
                     LTAIDTAGNTSQPSEVVSETVP"
BASE COUNT          690 a         1051 c         1210 g          749 t
ORIGIN      
        1 cgggcgcagc ggaagtgatc gcgtcggctt tcatcgaacg caaacgcggc gaggtggtcg
       61 gggaaaagag tttcggcgcg ggtgcggagc agcagttgtt tcctctacgc aacggcgacg
      121 ggctgctttt gacgaccgtg aaatgggctt catcggcggg caagtcgttt atcggagcgg
      181 gcgtgacgcc gacggtggaa gttaaacgac ccgaattagc cgccgcgatt gacccggacg
      241 aattgaccgg caacgacgac gatgcgattg ccgctcccgc accgccacag aatggcaggg
      301 aagtagcacc ggaagccact tcgcccaagc agcaacccga agacacacaa ctcaagaagg
      361 cgctcgaact gctcaacaaa ccggcgacgc agcagaaggc cgcttaaaat ttttcgaccg
      421 cattgacgaa aaaggccgga gcaggtggta atcctgctcc ggccttttcg cttgtcgcgc
      481 ccgtgccgcg cgtcgtgctc tgtccagccc tcgaacgggt tcagagcggg atgttgccgt
      541 gctttttcgg cgggttggtg tcgcgtttat tttcgagcag ggcgagggcg cgaatgagtt
      601 tgcggcgcgt ctcggcgggt tcgataattt cgtctatgaa gccgcgttcg gcggcgacat
      661 aagggttggc aaacttctct tcaaactccg tgatgcgtgc gcttctcgcg ctctccgggt
      721 ctgccgcttc gccgatctcg cgccggtaca ggacgccaac cgcgccgccc gcgcccatca
      781 cggcgatctc tgctgtaggc cacgcgagat taatgtccgt gcgaatgtgc ttggaggcca
      841 tgacgcaata agcgccgccg taagccttgc gcgtgatgac agtgatcttc gggacggtgg
      901 cttcggcaaa tgcgtagagc agttttgcgc cgtggcgtat gatgccgccg tgctcctgcc
      961 ggacgccggg aaggaagccc ggcacgtctt caaagacgac gagcgggatg ttgaagcaat
     1021 cgcagaagcg tacgaagcgc gcggccttga cggaagcgtc gatgtcgagc acgcccgcca
     1081 gatacgcagg ttgattggcg acgatgccga cgctgcggcc atcgaggcgc gcaaagccga
     1141 cgacgatatt cggcgcataa aactcgtgca cttcaaaaaa atagttctcg tctatcacgg
     1201 catgaatcac gtcgcggatg tcgtaaggtt gattcgacgc ttcgggcaca atcgagttca
     1261 agcgttcatc gcggcggtct gcggggtctg ccgtcgggac gcggggggct tcgtccatgt
     1321 tgttggaggg caggaaggag agtagttcgc gcacgagttg gagcgcgtgc tcgtcggagt
     1381 cggcgtcgaa gtgtgcgacg ccggagatgc ggttgtgcgt gtcggctccg ccgagttcct
     1441 ctttggagac ttcttcgtgc gtgacggcgc gaatcacctc cggacccgtg atgaacatgt
     1501 atgaagtgtt cttcaccatc acgttgaagt ctgtgatggc ggggctgtag accgcgccgc
     1561 ccgcgcatgg tccgaggatg cagctgattt gcgggacgac tccggaggcc agagtgttgc
     1621 gcagaaaaat gtccgcgtag ccgccgaggc taaggacgcc ttcctgtatg cgtgcgccgc
     1681 ccgaatcgtt gaggccgatg acgggcgcgc cgacgcgcat cgccaggtcc atcactttgc
     1741 agatcttttc ggcgtgcgtc tcggagagcg aaccgccgaa caccgtgaaa tcctgcgcga
     1801 agacgaagac gcgtcgatta tcgacgaggc cgtagcccgt cacgacgccg tcgccggggt
     1861 agagttgctc gccgagtccg aaatcgcgcg agcgatggac gacgtgcttg tcaaactctt
     1921 cgaaagttcc ttcgtcgagc agaaaggcga tgcgctcgcg cgcagttaat ttgcccgccg
     1981 cgtgctggcg ttcgatgcgc tcgcgcccgc cgccttcttc ggcgcgacgt tcgcgctcgc
     2041 gcagtcgctc tattttcgag agcgccggtt tcgtctccgt gcgtgttgaa gccataaatt
     2101 ttcgtgccgt ttaattattg ccggggcgga attgtacacc atgaataccg ctcgccgtgc
     2161 cgtatttaca tctgaccgtc aaaggggtgc tccctgcgct cgatgagcga gagcaattcc
     2221 gcgagcgcgt gccgcgcaga ctcgcgcacg gattcgggat aaccgtagag ggctgcgttg
     2281 gcggcaaact cttcttcgtc gagaatgcga taggagaaat ccggcgcgac gagtacgtct
     2341 atgtcgaggt ctgtgaaggt caggacgtgg ccgtcgaatt tcgcaggctg attgatgttg
     2401 cagtaatagt ttctcaatgc gccccacggt tcatggaaac gaaagacgct gtaccagcga
     2461 tccgtccagt agtattccgc gctcagcgtg ccgggggcaa tcgtgccgag ttggtcgtgt
     2521 cgtatctctt ctgcgaacac gccttcgacg acgacgagcg agccgacgcg ccggaccagt
     2581 ccggcctgcc attcacgatg caaacgcccg tcgaacttgc gcgcgtgaac cgtgacgggc
     2641 ggcctcgcct gcgccccctc cgcgcccgct gctgaacttt caacccccat gccgcctttt
     2701 gtcatccttt acctgccagc ccgcacggga ttggcgagca gccctacgtg ttcgacttcg
     2761 acttcgcaca cgtcgccgtc agccagaggt gcgacgcccg caggggtgcc ggtcgagatc
     2821 aggtcgccgg gttcgagcgt catgatgtgc gagatgtagc gtatgagata ggagacgggg
     2881 aaggccattg cgctcgtgcg cccgctctgg cgcacctctc cgttgacgcg cgtttcgacg
     2941 ccgaggtcga aagggtctaa gccgcacacg atgtatgcgc cgacggggca gaaggtgtcg
     3001 aatgatttgg cgcgcgtgaa ctgcacgtcg cggcgttgga ggtcgcgcgc ggtgacatcg
     3061 ttgacgcagg tgtagccgag cacatagtgg agcggatttt cgctatcgct cagacgatgc
     3121 gcacgccgtc cgataacgac cccgagttcc ccttcatgtt cgacgcgcga agagactgga
     3181 gggagttcga tggcgtcctg atgaccgacg acggatgaag gcggcttgag aaagagcaga
     3241 ggctcgtcgg gcatcgggtt gccgagttcg gcggcgtgct ctttgtagtt gcgcccgacg
     3301 cagacgatct tcgaaggcgc gacgggcgcg agcagcttta cttcggagag ggcgaaacct
     3361 gcgtccgcgc gggcgggcgc gtgcgacgag gcaaaaattt gcgctgcgga aaccggatag
     3421 accgtctcgt cttcaatcag gccgtagctc gcctcgcctt cgttcgggtt gagaaagcgg
     3481 cagatttttt tgctcattta aggcaccgtt tcggagacga cttcggaggg ctggctcgtg
     3541 ttgccagccg tatctatggc cgtcaggtag tagaaatatt tcgtgccgga ttcgaccgcg
     3601 tcgtcttgaa aagtggtgcg ctcaaggagc gtgcggttga gtttggtcca acgctctttc
     3661 ggcgcagacg ggtctgtgga acgatagacg ttgtagcccg
//
LOCUS       DASWNS010000078        10061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_313899, whole genome shotgun sequence.
ACCESSION   DASWNS010000078 DASWNS010000000
VERSION     DASWNS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313899"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VGB76_02685"
     CDS             <1..606
                     /locus_tag="VGB76_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY0097837.1"
                     /translation="DKIMVGEIRDNETAQIAIQSALTGHLVFTTVHANNVIDVIGRFL
                     NMGVEPYNFVSSLNCVLAQRLIRMLCDVCKRPYHPTEDELLESGLKPAEYRERTFYMS
                     VGCDACNHTGYRGRTAIHELLNLSDNVREMIIERRPGSEVRRAAEQEGLASLRESALK
                     KVFSGVSTLHEINRATFVEEVDLNGNGAGAKVNGDAQPQAV"
     gene            775..3660
                     /locus_tag="VGB76_02690"
     CDS             775..3660
                     /locus_tag="VGB76_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEY0097838.1"
                     /translation="MKLTVSVRGFASLVMVLCLLAAPVAVLAKKGEKNYKRGLEQERA
                     MQWERAAQEFALAVAANPSDIEYQLHYRRAIFNASQRFMEQGRTLAEQGDFSGAYNAY
                     RQAMGYDPINELAASEMERMLRLQREKEGRDPANANGNGNGAPNRSNGTPSTGAAVVP
                     TSYTGGTANGLGQRAQDALPAPRTEQLRVINYNGDLEGFIRKLGDELSLNVLFDRDFP
                     KGRTININLKDVTTARALDHIFLSQNLFFQKLDRRTILVADQSKRGQYQQLVLRTFYL
                     SNIDPNDARSLIQSSLPPTAGRQPQVTPNKATNSITVRDTPENIRLIAELLQAIDKDR
                     AEVVMDVNIYEVSRTNLLQLGNQLGTSDSLGNLGGLLPTPFLFGARKDRFGNTTTTTG
                     GTKGGTTTTGSLITNAIGGSFLLPASTITAFQSKDNTRLLAATQVHAFDSEQSTTRIG
                     QKVPVQTAQVSPFGSTIGGTQTGQQPGAASSLFGGSGYPVIQYQDTGLILKFTPQVFP
                     NLDVQVKMEIESNDVIGGATNLTPTFSQRNVSGMARIPNNRTMMIASVAQDKHSEGRQ
                     GIPLLGLIPILGRLFSTPTRNDVNSDIVITVTPRVLRAPTVSPGDIDQRPSGTLQAPV
                     SESLEALLVQADREDQLAAARQLPKDVALQVPTNAATTVAASPAAVVAPAETPEAQSY
                     APAPKALMALTDASAHHVAASNSSTAATAASAETAAPQANKSAEAVKPPAPVVSSATV
                     ALVPISNSGAAITPAAANLTTKTAAPPTVNAATPSTNTTPAGNASPVNNASPVPATVK
                     NATPPVVANAAASPSAPLATMTEGGTQLYLLSGQKEMRVGERQRLMLLVKTTTPLGLA
                     AATLRFDPRVFAVRGVTQGSLFGEAKEALPSITPSIDARGSLLALIAPATSAPISGMG
                     VLLFVEVEALAAGESDVTLDGAGVHLMSVSGQNVATQLANTRLTVKP"
     gene            3691..4398
                     /locus_tag="VGB76_02695"
     CDS             3691..4398
                     /locus_tag="VGB76_02695"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY0097839.1"
                     /translation="MAKGVGGELKGSGRRLCVWASRTFALAPRRSQGGWTLIELVITM
                     TVLTILSMGVIPMVKTSVKRQREQQLRESLREMREAIKEFHRDALNSPYLDAPQLQAQ
                     VPPQQQQPTVTTDPRSKMAIADKTLFGVDNPDRFPPTLETLVQGVSVAPRVQQVPGAT
                     ADGKLPELGSGSTLAFKKKIYLRKIPVDPITGKAEWGLRSCYDSPDSGSWGGENVFDV
                     YSKSQDTALNGQKYSDW"
     gene            4412..4936
                     /locus_tag="VGB76_02700"
     CDS             4412..4936
                     /locus_tag="VGB76_02700"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0097840.1"
                     /translation="MSSEQRAGSGGERGGRCVAALPAARSSMFTGLRRRRLALAPRLQ
                     RVAATRSGGFTLLELMIVISIIIILAIIVLPQYQKTVLQARESVLKDDLAQMRKLLDQ
                     YAADKGQLPQSLDDLATSGYLREVPIDPITGEKDWNVITGQDPNSAEGGQGVVDVRSS
                     SGDLSTEGTPYSEW"
     gene            5098..6072
                     /locus_tag="VGB76_02705"
     CDS             5098..6072
                     /locus_tag="VGB76_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097841.1"
                     /translation="MNAFSKLIKPRLPAAAVGLSGEGAALVSLDRRRDGSLLVRRAGY
                     VPLAEGLVQPGFDETNVADAREFAAVLQELSTSAGLQKRKRWSVALPEAATRTTILTM
                     EGGKASRAEMEEMLRWKTERGFGTPVEELRVARERLRPDGQGRARYLATAVRLTVLAE
                     YEDTFAALGWQTGLILPRHMGEAWWLMRGRVAAATVNAADSLLVSTHHEGFTAVLLRA
                     AEPLLVRSIVCDAEDRVDELYRFLLYYRDRVQTPASEGDAPLDTIGQMLLAGDGIEHT
                     EASAIIEETMGLRPRLITAADVRLALPSAELDFDQIAAPAGLAALAWA"
     gene            6332..6940
                     /locus_tag="VGB76_02710"
     CDS             6332..6940
                     /locus_tag="VGB76_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0097842.1"
                     /translation="MTATSPTVQKFPGVADCELVTRAIAGRDDSFEELVRRYQRPLVS
                     YVYRMTGNYESALDLTQEVFIKVYGSLARYRPEYKFSTWIYRIAHNTAIDHLRRHSTR
                     EQELEVTTTEGETYEKPYASSAPTPEEESVRAERRAEIEDVIQSLPASYRELIVLRHA
                     HDLSYDEIAEVTGLPLGTVKNRIFRAREAMRLPLVERGITGI"
     gene            6968..7573
                     /locus_tag="VGB76_02715"
     CDS             6968..7573
                     /locus_tag="VGB76_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0097843.1"
                     /translation="MSTQLNTVEPPEKPNACLDVAAYLDGELDAAEGALFEQHTKTCA
                     HCATALREQRRLLCVLDAAFGDAQQQIQLPANFTQVVKARAQSDMSSVRHRSERRRAL
                     LLCAGLAALSFALLGGQVWDELFAPLRAVAGVLLTMLDMAAHVLGEAVAGIALMLRVL
                     GGQLFDEPSAFSTVTCIILALAVVLLFRLIGSYHRTSRLPD"
     gene            7667..8665
                     /locus_tag="VGB76_02720"
     CDS             7667..8665
                     /locus_tag="VGB76_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097844.1"
                     /translation="MNTFILQPTTPPLATLRLLSRWCAVLCALLCAGVSVDARTPSVR
                     VAENDPTTLIVEGDDTNDVFGMGRSVIVRGRVKHGVTAFGGDVFVEAHGRVEGDVGVI
                     GGTITQRENSYIGGDVLVIGGAYHHDKAAPGRNPASKTVMYTGYEEELRELGRNPWSV
                     LEPKWTTASFGLRVLSVLFWFIVSLALTAATPGAISRAATRLQLTSLRVAAIGLLATI
                     VLLFGVPVALYLLPPVLGVFVGALALLFWVVAYLFGRVPIYAATGRWLQRLLLNETQR
                     SESIALLLGAIFWAVVLSLPYIWAFAFAGLVVTSLGLALTARYRIGWKSPVSAARS"
     gene            8995..>10061
                     /locus_tag="VGB76_02725"
     CDS             8995..>10061
                     /locus_tag="VGB76_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097845.1"
                     /translation="MKKQLLTFATLALVSSLSLVSAQIPATGTAASQPAQTDKAITAN
                     GVLGDVTAINTEAKGLSVKTDAGNTVTVVFSDTTSFIRAQPGATTLEGATKITPAEIS
                     VGDRVFARGKVADDQKSVMVRALVVMTKADIAKKHERDREEWRRRGILGTVTAVNPAT
                     KEITVSTSTPEGLKPLVVEAGGEKIMFRRYAPDSVKFADAKTSSFTELKVGDRLRALG
                     ERSADGARFTPEEIVSGAFRQLVGTVTAVDAATNEIKITTQDKRPLTIVVNQDSNLRR
                     LPAMMAQMLAARAAGGGAANGQGSGGTRPPQAGGEPGAGGNGRGGGGGGGGRRMGGFD
                     PQDMLERMPQLTLAELKPGDM"
BASE COUNT         1899 a         3284 c         3110 g         1768 t
ORIGIN      
        1 gacaagatca tggtcggcga aatccgcgac aacgagacgg cgcagatcgc catccagtcc
       61 gctttgaccg gccacctcgt gttcaccacc gtccacgcca acaacgtcat cgacgtgatc
      121 gggcgtttcc tgaacatggg cgtcgagcct tataatttcg tctcgtcgct caactgcgtg
      181 ctcgctcaac gcctgatccg tatgctctgc gacgtgtgca agcgccctta tcatccgacc
      241 gaggacgaac tgctcgaatc gggcttgaag cctgccgaat accgcgagcg cacgttctac
      301 atgagcgtcg gctgcgatgc ctgcaatcac acgggctatc gcgggcgcac cgcgattcac
      361 gaactgctca atctctcgga caacgtgcgc gagatgatca tcgaacgccg tcccggttcg
      421 gaagtccgcc gcgcggctga gcaggaaggt ctggcttctc tgcgtgaatc cgcgctcaag
      481 aaggttttct ccggcgtctc gacgctgcac gagatcaacc gcgccacgtt cgtcgaagag
      541 gttgacctga acggcaacgg cgcaggcgcg aaagtgaacg gcgacgctca gccgcaggcg
      601 gtctgaaagc catctgagcc gcctcgccgc tcgccgccga cggctcactt tgggctggct
      661 tttttggaac agtctctcgc gctcgtcgtg taaatacttc aggtggtcag ccccgcccgg
      721 taatgctgat tgctgaaatc ccaaactgaa cgctgattta tcttgaggat cgaaatgaaa
      781 ttaacagtta gtgtgcgtgg cttcgcttcg ctcgtcatgg ttttgtgcct gctcgccgct
      841 ccggtggcgg tgctggcgaa gaagggcgag aagaattaca aacgcgggtt ggagcaggaa
      901 cgcgcgatgc aatgggagcg cgccgcgcaa gagtttgcgc tcgccgtcgc cgcgaaccct
      961 tccgacatcg agtaccaact ccactatcgc cgcgccatct tcaacgcctc gcagcgtttc
     1021 atggagcagg ggcgcacgct cgccgaacag ggcgacttct ccggcgcgta taacgcctac
     1081 cgccaggcga tgggctacga tccgatcaat gaactcgccg cctccgagat ggagcgcatg
     1141 ttgcgcctgc aacgcgagaa ggaagggcgc gacccggcga acgcgaacgg caacggcaac
     1201 ggcgcaccca accgctcgaa cggcacgccg agcacgggcg cagccgtcgt ccccacgtct
     1261 tacacgggcg gcacggcgaa cggtctgggg caacgcgcgc aggatgcgct cccggctccg
     1321 cgcaccgagc agttacgcgt catcaactac aacggcgacc tcgaaggctt catacgcaaa
     1381 ctcggcgatg aactgagcct caacgttctc ttcgaccgcg acttcccgaa agggcgcacc
     1441 atcaacatca acctgaaaga cgtgacgacg gcgcgcgcgc tcgaccacat cttcctctcg
     1501 cagaatctct tctttcagaa actcgaccgc cgcaccattc tcgtcgcaga tcaatcgaag
     1561 cgcgggcagt atcagcaact cgtcctgcgc acgttctacc tgtcgaacat cgacccgaac
     1621 gacgcgcgct ctctgattca atcttccctg ccgccgaccg ccggacgcca gccgcaggtg
     1681 acgcccaaca aagccaccaa ctccatcacc gtgcgcgaca cgcccgaaaa catacggctc
     1741 atcgccgaac tgctgcaagc catcgacaag gatcgcgcag aagtcgtgat ggacgtgaac
     1801 atctacgagg tttcgcgcac gaacctgctg caactcggca accagctcgg cacttccgac
     1861 tcgctcggca acctcggcgg cctcttgccg acgcctttcc tcttcggcgc gcgcaaagat
     1921 cgtttcggca acacgaccac gacgacgggc ggcacgaagg gcggcacgac cacgaccggc
     1981 tcgctcatca cgaacgccat cggcggttcg ttcctgttgc ccgcttcgac catcaccgcg
     2041 tttcaatcga aggacaacac gcgtctcctc gcggccacgc aggttcacgc tttcgacagc
     2101 gaacaatcca ccacgcgcat cggccagaaa gtccctgtgc agacggcgca ggtgtcgccc
     2161 ttcggctcga ccatcggcgg cacgcagacg gggcagcagc cgggcgcggc ctcttcgctc
     2221 ttcggcggtt cgggctaccc ggtcatccag tatcaggaca caggcttgat cctgaagttc
     2281 acgccacagg tcttcccgaa cctcgacgtg caggtgaaga tggagatcga gtcgaacgac
     2341 gtgatcggcg gcgcgacgaa cctgacgccg acgttcagcc agcgcaacgt ctccggcatg
     2401 gcgcgcattc ccaataaccg cacgatgatg atcgccagcg tcgcgcagga taagcattcc
     2461 gaagggcgac agggcatccc gctcctcggc ctcatcccca ttctcggcag gctcttcagc
     2521 acgccgacgc ggaacgacgt aaactccgac atcgtgatca cggtgacgcc gcgcgttctg
     2581 cgtgcgccga ccgtcagccc cggcgacatc gatcagcgtc cgagcggcac gctgcaagcg
     2641 cccgtctccg aatcgctcga agccctgctt gtgcaggccg accgcgaaga ccaactcgcc
     2701 gccgcgcgcc agcttccgaa agatgtcgcg ctgcaagtgc cgacgaacgc cgctactacg
     2761 gttgcggcat cgcccgccgc agttgtcgcg cccgccgaga cgcccgaggc tcaatcctac
     2821 gcgcccgcgc cgaaagccct catggccttg acggacgcga gcgcacatca cgtcgccgcc
     2881 agcaacagca gcacggctgc gacggcagcc agcgcggaga cagctgcgcc gcaggcgaat
     2941 aagagtgcgg aggccgtaaa accgcccgcg cccgtcgtct caagcgcgac ggttgcgctc
     3001 gttcccatct ccaattccgg cgcggccatc acgcccgccg ccgcgaactt gacgacgaag
     3061 accgccgctc cgccgacggt caacgccgcg acgccttcaa ccaacacgac tccggcgggc
     3121 aatgcgtcgc cggtaaataa cgcgtcgccc gttccggcga ccgtgaagaa cgcgacgccg
     3181 cccgtcgtcg ccaatgctgc tgcttctccg tctgcgccgc tcgcgacgat gacggagggc
     3241 ggcacgcaac tctatctcct ttcgggacag aaggagatgc gcgtcgggga gcgtcaacgc
     3301 ctgatgctgc tcgtcaagac gacgactccg ctcggactcg ccgccgcgac gctgcgcttc
     3361 gacccgcgcg tctttgccgt gcgcggcgtc acgcagggca gcctcttcgg cgaagcgaag
     3421 gaagccttgc cctcgatcac gccttcgata gatgcgcgcg gctctctgct cgcgctcatc
     3481 gcgcccgcta cgagcgcgcc gattagcggc atgggcgtcc tgctgttcgt cgaagtcgaa
     3541 gctctggcgg caggcgaaag cgatgttacg ctcgacgggg cgggcgtcca tctgatgtcc
     3601 gtcagcgggc aaaacgtggc gacgcaactg gcgaacacgc ggctcacggt gaagccgtaa
     3661 gcggcatgag cgaaacgggc gggagcgatt atggcaaagg gtgtgggggg agagttgaaa
     3721 ggtagcggca gacgcctctg cgtttgggct tcgcgcacgt tcgccctcgc cccgcgccgt
     3781 tcgcagggcg gctggacgct catcgaactc gtcatcacca tgacggtgct cacgattctc
     3841 tcgatgggcg tgatcccgat ggtcaaaacc tccgtcaagc ggcagcgcga acaacaactg
     3901 cgcgagtctc tgcgcgagat gcgcgaagcg atcaaggagt ttcaccgcga cgcgctgaac
     3961 agtccttatc tggatgcgcc gcaacttcag gcacaggttc cgccgcagca gcaacagccg
     4021 actgtgacca cagacccgcg cagcaaaatg gcgattgccg acaagaccct gtttggagtt
     4081 gataaccctg accgctttcc gccgacgctg gaaactctcg tgcagggcgt cagcgtcgcg
     4141 ccgcgcgtgc agcaggttcc cggcgcaacc gccgacggca aactgccgga actgggcagc
     4201 ggttcgacgc tcgccttcaa gaagaagatt tacctgcgca agattcccgt cgatccgata
     4261 acggggaagg ccgagtgggg attgcgttcc tgttacgatt cgccggattc cggatcgtgg
     4321 ggcggcgaaa acgtctttga tgtctattcg aaatcgcagg ataccgcgct gaacgggcag
     4381 aaatacagtg attggtaaaa ggttaaaaat tgtgagcagc gaacagagag caggaagcgg
     4441 cggggagaga ggcggacggt gtgtagctgc gctgcccgct gcgcgctcct ctatgttcac
     4501 aggcttgcgg cgtcgtcgcc tcgctctcgc accgcgtcta cagcgtgtcg cggcaacgcg
     4561 ctcgggcggg ttcaccctgc ttgagttgat gatcgtgatc tcgatcatca tcattctcgc
     4621 catcatcgtg ctgccgcaat accagaagac ggtgttgcag gcgcgcgagt cggtcttgaa
     4681 agacgatctg gcgcagatgc gcaagctgct cgatcaatac gccgccgaca agggacaact
     4741 cccgcagtcg ctcgacgacc tcgccacctc cggttacctg cgcgaagtgc cgattgatcc
     4801 gatcaccgga gagaaggact ggaacgtgat cacggggcaa gacccgaact ctgcggaggg
     4861 cgggcagggc gtcgtagacg tgcgtagttc ctccggcgac ctctcaaccg aaggcacgcc
     4921 atacagcgaa tggtgatagg gcagtgacga atgacgagtg acaagttaaa actaagaatt
     4981 aagaacttgt cttcaactcg tcttcaactc gtcgctcgct actcgtcacc tgtccggcat
     5041 caatgagttt ctcttttaac ttgtcactcg tcgcccgtca ctcgtcactg ttctcttatg
     5101 aatgctttct caaagttgat taaaccgcgc ttgcctgcgg cggcggtggg cttgtcgggc
     5161 gagggcgcgg cgctcgtctc gctggatcgc aggcgcgacg gctcgctgct cgtgcggcgc
     5221 gcgggttacg tgccgctcgc cgaggggctg gtgcagccgg gtttcgacga gacgaacgtg
     5281 gccgacgcgc gcgaattcgc cgccgtgctg caagaactct cgacgagcgc gggcttgcag
     5341 aagcgtaagc gttggtctgt ggcgttgccg gaagcggcga cgcgcacgac gatcctgacg
     5401 atggagggcg gcaaggcttc gcgcgccgaa atggaagaga tgttgcgctg gaagaccgag
     5461 cgcggcttcg gcacgccggt cgaagagttg cgcgtggcgc gcgagcgcct gcgcccggac
     5521 gggcagggtc gcgcgcgtta tcttgcgacg gcggtgcggc tcacagtgct cgcggaatac
     5581 gaagatacgt ttgccgcgct cggctggcag accggtttga ttctgccgcg ccacatgggc
     5641 gaggcgtggt ggctgatgcg cgggcgcgtg gccgccgcta ctgtcaacgc agccgactcg
     5701 ctgctcgtca gcacgcacca tgaaggcttt accgccgtcc tgctgcgcgc cgccgaaccg
     5761 ctcctcgtgc gcagcatcgt ctgcgacgcg gaagaccgcg tggacgaact ctatcgcttt
     5821 ctactctact accgcgaccg cgtgcagacg cctgcctcgg aaggcgacgc gcccctcgac
     5881 accatcgggc agatgctgct cgccggagac ggcatcgagc acacggaagc gagcgcgatc
     5941 atcgaagaga cgatgggctt gcgcccgcgc ctcatcaccg ccgccgacgt gcgcctcgcg
     6001 ctcccgtccg ccgaactcga cttcgaccag atcgccgccc ccgccggact cgccgccctc
     6061 gcgtgggctt aaagtcgttc gcgtcatgtt cgcttgaggg cttcgagcga cgcgcggcat
     6121 caggcgagag gatgccggat gaggggagtg gctgtgcagg ggaacgcggg cggcggaaag
     6181 tctacgctcg ccgcgagaga tcggatcgtt ttgcgcggcg caatctttaa taaaggcgcg
     6241 gcgacgcctc gcccgccaga gtatgcggtt cgagcgcgcg agtaatgaaa ctttcgcttt
     6301 tgtgccaacg taaagacgtt ttatcgagtt tatgaccgca acttccccca ccgtgcaaaa
     6361 gttccccggc gtcgccgact gcgagttggt gacgcgtgcg atagccgggc gcgacgacag
     6421 tttcgaggaa ctcgtccgcc gctaccagcg accgctcgtc tcctacgtct atcgcatgac
     6481 gggcaactac gagtccgcgc tcgacctgac gcaggaagtg ttcatcaagg tctacgggtc
     6541 gctcgcccgc tatcgccccg aatataaatt ttccacctgg atttatcgca tcgcgcacaa
     6601 cacggcgatt gaccacttgc gccgccactc gacccgcgaa caggaactcg aagtgacgac
     6661 caccgaaggc gagacttacg aaaagcctta tgcctccagc gcgccgacgc ccgaagaaga
     6721 gagcgtgcgc gccgagcgtc gcgccgagat cgaggatgtc atacagtctc tgcctgcgag
     6781 ctatcgtgag ttgatcgtgt tgcgccacgc gcacgatctc agttacgacg agatcgcgga
     6841 ggtgacgggg ctgccgctcg gcacggtcaa gaatcgcatc ttccgcgcgc gtgaggcgat
     6901 gcgcctgccg ctcgtcgaac gcggcatcac gggcatttga ggagcgcgcc gcgctggcag
     6961 agagatcgtg agcacccaac tcaacaccgt cgaaccaccg gagaaaccga acgcgtgtct
     7021 cgacgtagcc gcgtatctcg acggcgagtt ggacgccgca gagggcgcgc tcttcgagca
     7081 gcacacgaaa acctgcgcgc actgcgcgac cgcgctccgc gagcagcggc gtctgctgtg
     7141 cgtcctcgac gccgcgttcg gcgatgcgca gcaacaaatt caactccccg caaatttcac
     7201 gcaggtcgtc aaagcgcgcg cgcagtcgga catgagcagc gtgcggcatc gttcggagcg
     7261 gcggcgcgct ctgctgttat gcgcgggact ggctgcgctc tccttcgcgc tgctcggcgg
     7321 gcaggtctgg gatgagttgt tcgcgccgtt gcgcgccgtc gcgggcgtgc tcttgacgat
     7381 gctcgacatg gcggcgcacg tcctcgggga agccgtcgcc ggaatcgcgc tcatgctgcg
     7441 cgtgctcggc gggcagttgt tcgacgaacc gagcgcgttc agcaccgtca cctgcattat
     7501 tctcgcgctc gccgtcgtgc tgctcttccg gctcatcggg agttatcatc gcacctcgcg
     7561 cctccccgac taaaagaaaa ctctccgcgc taaaactctc cgcccctttc atacgtaaca
     7621 actctggctt attcgactat agctctgccg ggacgcgatg acagacgtga acacgttcat
     7681 tctccaacca actacgccgc cgcttgccac tttgcgcctc ctttcgcgct ggtgcgcggt
     7741 tctctgcgcg ctcctgtgcg ccggagtctc ggtcgatgcg cggacgcctt ccgtgcgcgt
     7801 ggcggagaac gatccgacga cgctgatcgt agaaggcgac gataccaatg atgtgttcgg
     7861 gatgggtcgt tcggttatcg tgcgcgggag agtcaagcat ggcgtgacgg cctttggcgg
     7921 cgatgtgttt gtcgaagcgc acgggcgcgt cgaaggagac gtgggcgtca ttggcggcac
     7981 gatcacgcag cgggaaaatt cttacatcgg cggcgacgtg ctcgtcatcg gcggcgcgta
     8041 tcatcacgac aaggctgcgc ccggacgcaa tccggcctcg aagacggtca tgtacacggg
     8101 ctacgaagag gagttgcgcg aactgggacg caacccttgg tcggtgctcg aaccgaaatg
     8161 gacgacggcc tccttcggcc tgcgcgtcct gtccgtcctc ttctggttca tcgtctctct
     8221 ggccttgacc gccgccacgc cgggcgcgat cagtcgggcg gccacgcgtt tgcagttgac
     8281 gagtctgcgc gtcgcggcga ttggcctgct ggcgaccatc gtgctcttgt tcggcgtgcc
     8341 ggtcgcgctc tacctgttgc cgcccgtgct cggcgtcttc gtgggcgcgc tcgcgctcct
     8401 gttctgggtc gtcgcctatc tcttcgggcg cgtcccgatc tacgcggcca ccgggcgttg
     8461 gctgcaacgc ctgttgctca acgaaacgca gcgttccgaa tcaatcgccc tgctgctcgg
     8521 cgcaatcttc tgggcggtcg tgctgtcgct gccctacatc tgggcgttcg ccttcgccgg
     8581 tctggtcgtg acgagtctgg ggctggctct gacggcgcgc tatcgcatcg gctggaagtc
     8641 gcccgtctcc gccgcgcggt cttagactca cccgccgcgt tcgtcaatct caccgttaaa
     8701 tccccggcgt tccctgaaat tccgcgaaat ttgcgcaaac gaaggcgact aaaagaggcc
     8761 gtcgcgcgtc taacctctcg cggcgaacgc acgctcccca tactccccaa actccctgtt
     8821 gacgaaactt tcccgccatt tgagacgtga gacgcgccgg agcggcgcac gcgccgggaa
     8881 ccgtcggctt tgcgcgattc gacaaaacgt gcgaaaacgg caacactatt ggctcgcaga
     8941 tgtaaaacat taatcgtttc gagcgtttat ctactgaccg aaaagagcat caagatgaaa
     9001 aagcaacttc tgaccttcgc caccctcgcg ctcgtatcga gcctgagcct cgtttccgcg
     9061 cagattcccg ccaccggcac agccgcgtcg caaccggcgc agaccgataa agcgatcacc
     9121 gcgaacggcg tgctcggcga cgtgacggcc atcaacacgg aagcgaaagg tttgtccgtc
     9181 aagaccgacg cgggcaacac cgtcaccgtc gtcttcagcg acaccacctc cttcatacgc
     9241 gcgcagcccg gcgcgaccac gctcgaaggc gcgaccaaaa ttacgcccgc cgagatcagc
     9301 gtcggcgacc gcgtgtttgc gcgcggcaaa gtggctgacg atcaaaagag cgtgatggtg
     9361 cgtgcgctcg tcgtgatgac gaaggccgac atcgcgaaga agcatgagcg cgaccgcgaa
     9421 gagtggcgac ggcgcggcat cctcggcacg gtcacggcgg ttaatcccgc caccaaagag
     9481 atcactgtct ccacctccac gcccgaaggc ttgaagcctc tcgtcgtgga ggcggggggc
     9541 gagaagatta tgtttcgtcg ttacgcgccc gactccgtga agtttgcgga tgcgaagacg
     9601 agcagtttca ccgagttgaa ggtcggggat cgtttgcgcg cgctcggcga acgcagcgcg
     9661 gacggcgcgc gcttcacgcc cgaagagatc gtctccggcg cgtttcgtca actcgtcggc
     9721 acggtcacgg cggttgacgc ggcgacgaac gagatcaaga tcacgacgca ggacaagagg
     9781 ccgctcacca tcgtcgtcaa tcaggattca aacctgcgtc gcctgccggc gatgatggcg
     9841 cagatgcttg cggcgcgcgc agcgggcggc ggcgcggcga acggtcaggg cagcggcggc
     9901 acgcgtcctc cgcaggcggg aggcgagccg ggcgcaggcg gcaacggtag aggcggcggt
     9961 ggcggcggag gcggacgccg gatgggcggt ttcgacccgc aggacatgct cgaacggatg
    10021 ccgcaactga cgcttgccga gttgaaaccg ggcgacatga t
//
LOCUS       DASWNS010000079         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_211252, whole genome shotgun sequence.
ACCESSION   DASWNS010000079 DASWNS010000000
VERSION     DASWNS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211252"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="VGB76_02730"
     CDS             complement(<1..1402)
                     /locus_tag="VGB76_02730"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY0097846.1"
                     /translation="MSTNEPVLRMKGITKIFSGVTALSNVDLALERGEVHALVGENGA
                     GKSTLIKIMTGAYTRDGGQVFLEGRAVDFRTPEDAQGAGVVAVYQEVNLLTYRTVAEN
                     IYLGREPRRFGLIDWKRMNADSGALLKRLGLEIDPRAPLGSLNIALRQMVAIARGVSF
                     DAKVVVLDEPTSSLTEHEVQILYDVIRRLKAEGTAVVYISHRFDELYTVCDRVTILRD
                     GKFVATRPLAGLEKIDLVCLMLGKQRDELRQGTTAFKHAGANAGADATPLLSAENLKR
                     GRKLNDVTVEVARGEIVGMAGLLGSGRTETARAIFGADSVEEGEVKLEGKKIKRGSVN
                     EAIDSGIAFLTEDRKAEGIIPELSVRENLTLAALPALSTLGVVSRARQAEIVDKFMKR
                     LGIKAANADQKIRELSGGNQQKVLLARWLCKNPKFLILDEPTRGIDIGAKGEIQSLVG
                     ELAESGLGVLMISSELE"
     gene            complement(1547..>2366)
                     /locus_tag="VGB76_02735"
     CDS             complement(1547..>2366)
                     /locus_tag="VGB76_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0097847.1"
                     /translation="ETRSIEGEAAKHADKYDLRVTVAQGDTAKQVSNVEDLVSQKVDA
                     ILLAPREKEGFEQALQAAKSANIPVILVDREASGTAGEDFVTVLKSDFVKQGQRAAEW
                     LAKASNGKANIVELSGTSGASVTADRAKGFADEIAKHPEMKIIQTQTGDFNRAKARDV
                     MQNIIQARGKEITAVYAHNDEMALGAIQALEAAGMKPGTEVLIVSVDGQKSALQAIID
                     GKMNVTIESNPRFGPLAFATLEKFWAGEKLPPLIGIEDRLFDKTNAAQFLNEAY"
BASE COUNT          372 a          798 c          685 g          511 t
ORIGIN      
        1 cttccaactc cgaagagatc atcaacacgc ccagacccga ttcggcgagt tcgcccacga
       61 gcgactgaat ctcgcccttc gcgccgatgt ctatgccgcg cgtcggttcg tcgagaatga
      121 gaaacttcgg gttcttgcag agccagcgcg cgagcagaac tttctgttga ttgccgcccg
      181 acaactcgcg tattttctgg tcggcgttcg cggccttgat gccgagccgc ttcataaact
      241 tgtctacgat ctcggcctgc cgcgcgcgcg agacgacgcc gagcgtggag agcgcgggca
      301 gcgcggcgag cgttaaattc tcgcgcacgc tcaactccgg gatgatccct tcggccttgc
      361 ggtcttccgt gaggaacgcg atcccggaat caatcgcctc gttcacagag ccgcgcttga
      421 tcttcttacc ttccaacttc acttcgcctt cttccacgct gtccgcgccg aagatcgcac
      481 gcgccgtctc cgtgcgtccc gaaccgagca agcccgccat gccgacgatc tcgccgcgcg
      541 ccacctccac cgtcacgtcg ttcagctttc gcccgcgctt caaattctcc gcgctgagaa
      601 ggggcgtcgc gtctgctccg gcatttgcgc ccgcgtgttt gaaagcggtc gtaccctgtc
      661 gcagttcgtc gcgctgcttg ccgagcatga ggcagacgag atcgatcttc tcaagccctg
      721 cgagcgggcg cgtggcgaca aacttcccgt cgcgcaggat cgtcacgcgg tcgcagaccg
      781 tgtacaactc gtcgaagcga tggctgatat agacgaccgc cgtgccttcc gctttcagcc
      841 gccggatcac gtcatagaga atctgcacct catgttccgt cagcgaactc gtcggctcat
      901 cgaggaccac cactttcgcg tcgaacgaga cgccgcgcgc aatcgccacc atctgccgga
      961 gcgcgatgtt gaggctgccg agcggcgcgc gcgggtcgat ctctaagccg aggcgtttga
     1021 gcagcgcgcc ggagtccgcg ttcatacgct tccaatcgat gaggccgaag cgtcgcggct
     1081 cgcgccccag atagatgttc tcggccacgg tgcggtaggt cagcaggttg acctcctgat
     1141 agacggcgac gacgcccgcg ccctgcgcat cttccggcgt gcgaaagtct acggcgcgcc
     1201 cttcgagaaa gacctgcccg ccgtcgcgcg tgtacgcgcc cgtcatgatc ttgatcaggg
     1261 ttgacttgcc cgcgccgttt tcgccgacga gcgcgtgcac ctccccgcgc tccaacgcca
     1321 gatcgacatt cgacagcgcg gttacgcccg aaaagatttt cgtgatgccc ttcatgcgga
     1381 ggactggctc gttcgtgctc ataagaattt gtccatcgcc ggttgcccgt ggccggttac
     1441 ccgtggcctg tcgttttcgt ctcgcccgga ttcgagactc ttatctttag cccgccgctc
     1501 ttgaaccaca acgacgtgca accggccaca ggcagcggac ggcttttcag tatgcttcgt
     1561 tcaaaaactg cgccgcgttc gtcttgtcga agaggcgatc ttcgatgccg atgaggggcg
     1621 gaagtttctc gcccgcccag aatttttcga gcgtggcgaa ggccagtggt ccgaagcgcg
     1681 ggttggactc gatggtcacg ttcatcttgc cgtcgatgat ggcttgcagc gcgctcttct
     1741 gcccgtcaac cgagacgatc aggacttccg tgcccggttt catcccggcg gcttcgaggg
     1801 cttgaatcgc gccgagcgcc atctcgtcgt tgtgcgcata gacggctgtg atctctttgc
     1861 ctctggcctg aatgatgttc tgcatcacgt cgcgcgcctt cgcgcggttg aagtcgcccg
     1921 tctgcgtctg gatgatcttc atctccgggt gcttcgcgat ctcgtcggca aaacccttcg
     1981 cgcgatccgc cgtgacggac gcgccgctcg tgccgctcag ttcgacgatg ttggctttgc
     2041 cgttcgacgc cttcgccagc cactcggcgg cgcgctgtcc ctgcttcacg aagtcggact
     2101 tgagcacggt gacgaaatct tctcccgccg tgcccgacgc ttcgcggtcg acgaggatga
     2161 cgggaatgtt cgcgctcttg gccgcctgca acgcctgctc gaacccttct ttctcgcgcg
     2221 gcgcgagcag gatcgcatca accttctgcg agacgaggtc ttcgacgttc gacacctgct
     2281 tggccgtgtc gccctgcgcg accgtcacgc gcaggtcata cttgtccgcg tgcttggccg
     2341 cttccccttc gatgctcctc gtctcg
//
LOCUS       DASWNS010000080        12409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_418743, whole genome shotgun sequence.
ACCESSION   DASWNS010000080 DASWNS010000000
VERSION     DASWNS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418743"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..2244
                     /locus_tag="VGB76_02740"
     CDS             49..2244
                     /locus_tag="VGB76_02740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY0097848.1"
                     /translation="MRRSTPLTLLLVYSLLLSTTGALPLPATHAQVSERRGNSLPGKM
                     SDSLRRRAKASRQSNKDGDGGERARVILNLAGDSAAVKQSRALLQGAGANVRQELNEL
                     GMMVADVPVERLEELAAHSEVAWMSEDAEVRTLATTDNTSHLELTTGASKVLPSDVTK
                     GGAGNGVGIAFLDSGISRPDAAEFAGYTLDTSGGLLGGGGLLGSGLLSTPTLTPYNRI
                     TKEMDFTGEGTKLDNYGHGTHAAGVAAGTGQASEEYAASHAGAPTYGGIAPMANLISV
                     RVLNSQGVGTVSGVIAGINWVIQNKSTYKIRVMNLSLGTSITQSYKTDPLCQAVGRAV
                     DAGIVVVVAAGNLGKDSNGQTIYGGILSPANSPKVITVGATNTQDTVKRSDDVVTTYS
                     SRGPTLVDGLVKPDLVAPGNRIGAAESSVTSPTASSSYSTSGGGGLLGGLVLGSPTTT
                     KPIATGTYRVWSGTSFSAPAVSGTVALMLEANPSLTPRIVKALLMRTAQRLPYYETSV
                     MTGQMSAYERIITEGAGELNANAAVALARAVRRDANLARTGENLITNSKLTYATVPGI
                     TSPIGGEAIPLNNGLVWSDGVAFNSRLQLTNGYRLSDGRTITSGFAIADGYLNADGSY
                     VLGNGIVVASGIVVASGIVVASGIVVASGIVVASGIVVASEYVLADGSFSTGALNGCS
                     FNYEAVWASNLVDPGTVDIKPDQVILLGEDAVPVGVRRLANVSPLYPRR"
     gene            2370..4484
                     /locus_tag="VGB76_02745"
     CDS             2370..4484
                     /locus_tag="VGB76_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0097849.1"
                     /translation="MTPDNAKVKSNDLYTTLLTVVSLALWLAASVTFFVEHAWREQLA
                     VLALLPLVIVTGMFPNTLPLPSALKLSEERMNFTLSDALVLLIACRYGFAACVFVAGI
                     EGLTSSRRVVRRLSSNLYSFGMMSLAAAAAAASLHAVLRYGFGEAAGTEHAFPAIAVA
                     VLAASIVHISVNTGVISLLFATRLGVPVMSSWKENFRLAVPLFLPTSAAASLMYIGLQ
                     YNALTMVAIGAPLLFAIYFGHRRYRDSMQEQLNLIERAQQERISTLEQAHRETIEALA
                     VTINAKDEVTHEHVLRVQIYAAGVARLLGCSDAEIEALRAGALLHDIGKIAVPDYIIK
                     KPGKLTAAEFEKMKIHTLVGAQILGRVGFSYPVVPVVRSHHERWDGKGYPDGLAGDSI
                     PLTARILSVVDCFDAVREDRQYRKGMTRTEAIDYLMGQSGTQYDPRVVGTFITHLPEF
                     EAEIAAHRNTTAPTYGIEPVEQLSEAARRVAPAAGLAEASAAPHPQTAEVEFTEAERD
                     ALSHAARRLSEARDTDEVIASFIESVKTLVPYDSCALTLAPPHTDESHMAYADGRHAD
                     LLNNFRIIPGEGVTGWVLANRQPFYNADPLLDLPPALAARDDSYRTLAVCPILRGVEL
                     HGVVTLYSATLRTYEPAQQQLIEALASLLAARLSANAQVASPTVLEISPPATAHLSQG
                     ELAFNMPPPHLAGVALESELAH"
     gene            4743..5309
                     /locus_tag="VGB76_02750"
     CDS             4743..5309
                     /locus_tag="VGB76_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEY0097850.1"
                     /translation="MRELKQTGIDNNAVATRTATGTLTPERRHTHVEIEQSLDQLSRW
                     MDGLFRVPGTSWRFGLDAIVGLIPGVGDTVTTIVGFYILAAGVRYRVSKATLLRMGLN
                     IGVDYIFGAIPVLGDLFDTAWKSNQKNVELLRRRATVTAEEARSGRLSDWLFVGLVML
                     CLLALLVGSITILWFILRFLSDQFSALF"
     gene            complement(5412..5888)
                     /locus_tag="VGB76_02755"
     CDS             complement(5412..5888)
                     /locus_tag="VGB76_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY0097851.1"
                     /translation="MPRIGTDMPSLEGATEWFNATGARAEAEAKGQPTLVHFWSVNCD
                     ACKGNMARVAAWRDERAADGLRVIAVHTPREEAEADTETVREGVALCNITEPCAVDNE
                     YKLRDAFQNEQGDVPAYYLFDGEGKLRSFASGEQGLEEVAAVLERVLAEHTGQAAS"
     gene            complement(6087..7166)
                     /locus_tag="VGB76_02760"
     CDS             complement(6087..7166)
                     /locus_tag="VGB76_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0097852.1"
                     /translation="MLSQETIFLTGFPGFIAARLIKELARDASSTRFVLLVQPAFLSR
                     AREEVARLATETGASAERFRIVEGDITSEARLGMSEAEFARASAETNVLFHLAAIYDL
                     AVRREPAMRVNVEGTRHVNEFARACKHLTRYHYVSTCYVAGLREGLIREDELAHTAGF
                     RNFYEETKYLAELEVERLKGELPVTIHRPAVVCGDSRSGETAKYDGVYYLIKYLRIWP
                     GLFSLVNIGNRDVRLNIVPVDFVVEAMAALARDASAVGATVQLADPQPLTTSELFETI
                     AHALVGRGSRVQLPKPLVRATLGLPFSEKLTKLPHIGVPYFFIKQTYDTSRAVELLAP
                     HHIACPPFPSYVRALVDYVARHPTL"
     gene            7387..8973
                     /locus_tag="VGB76_02765"
     CDS             7387..8973
                     /locus_tag="VGB76_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0097853.1"
                     /translation="MQFPRPLVVRRHTLLALALTIVSAVSTYAQANAQANVQATATPS
                     PQAGETVRVTLLQVNDVYQISPVDRGRRGGLARLATLRKIVLEESPHTLYLLAGDTLA
                     PSVASNIFKGRQMIAAWNATGLDYAALGNHEFDFGDAVLRERMRESKFTWLGSNVFDR
                     KTGKLFGDMPPFVVREFGGVKVGLFGLLTVDTKQTSSPGADVEFRDPCRTANETIKQL
                     RQRGVRTIVAITHLTIENDKRLARCAPGIDVIIGGHEHSVLQSLSERTPIFKMGSDGR
                     NLGRIDLNIYRRNGTVESVDWEIIPVTDGVEEEPKSAAIIAEYEKKLSAELDLPVGRT
                     TVELDARQETNRSRETNLGSFIADSYRKYAKADVAIVNGGSIRSNTTYGPGTITKRDV
                     LSILPFENPIVKVEVTGATLRAALEHGVSRIREEKEAGLFPQVSGLRFTYDGRLAPGA
                     RVKSVTVNGAPLDNGKTYTLAVNTYVLGGGDGYTMFKDAPLLLKPEEAQVEPAMLMNI
                     IAAAAEIAPATDGRIERSDK"
     gene            9118..9618
                     /locus_tag="VGB76_02770"
     CDS             9118..9618
                     /locus_tag="VGB76_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0097854.1"
                     /translation="MFEEDTLLSGAKSEAVELFRRAYEAQQSQAYAEAIELYRRSIET
                     YPTAEAHTFLGWAYSFLERYDEAIDACLEAIRVDPAFGNPYNDIGSYLIAKGDLYNCV
                     RWFKRALEAARYESYAFPHFNLGRVYEQRGHLLKAARHYGLALKQAPDFAQATKALRR
                     LQARLN"
     gene            9664..10155
                     /locus_tag="VGB76_02775"
     CDS             9664..10155
                     /locus_tag="VGB76_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097855.1"
                     /translation="MNTYLLTWSPKKWQWTDLSQRIREITERGFSLTDWSCGNNKSIA
                     RGDRLFLLRQGEEPRGIVGAGFAASEPYAEIHWREEKARVGKTTMYVKVRWEILLNPE
                     SESIFPREWLNEGFLSKVNWNTQISGISIRSEAADILEERWEAFLESRENEFSLFSNA
                     SPD"
     gene            complement(10224..10376)
                     /locus_tag="VGB76_02780"
     CDS             complement(10224..10376)
                     /locus_tag="VGB76_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097856.1"
                     /translation="MRIEVAEAGVVYVSVGANRRKMRLNRELFKGDLSGIMSGIVSGI
                     EPHETE"
     gene            10594..>12409
                     /locus_tag="VGB76_02785"
     CDS             10594..>12409
                     /locus_tag="VGB76_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0097857.1"
                     /translation="MPLTTGTQLGRYEICSLLGMGGMGEVYLAYDSRLRRKIALKVLP
                     DSLIDSPEHLRRFEREARAVSALNHPNIITIHEIGTDGDTHFIAMELIEGETLRQKLR
                     TGRLEIEQTLDVVTQIASALNAAHGSGIVHRDLKPENIMLREDGLVKVLDFGLAKLVE
                     KRGALPGDTQATTLAIGLTQPGAVIGTVAYMSPEQARGQEVDARTDVFSLGLVWHEML
                     SGSITFAGATASDMIAAILKSEPPPLSHFNQNIPAELERIVGKSLAKDREERYQTAKD
                     LLVDLRRLLKKLETQNEIGRTSPPDKTEHENATQILPARPARSGDKVSGASRTYRLGF
                     AIASGVLLLACVVWGYWFIAGRTSQLGQIESIAVLPFINESGNSDVEHLSDGMTETLI
                     NSISKLPLLSVKARSSVFHYKGKEIKPESIASELSVQALLTGRFVQRGNDLTLYLALV
                     DGRNGNQLWGEQYNRKLVDLVLLQSEIARDVSRKLRARLSRADEQEVVRNDTDNPEAY
                     ELYLKGRYHVFKLTPPEVDKGISYLQKAIHLDPNYALAYVGLSQAYRARVLGGEMNPA
                     ETLPKAKEAAQKAIRIKDALAEAHGALANMTFWYDWNWN"
BASE COUNT         2525 a         3965 c         3610 g         2309 t
ORIGIN      
        1 atccgccgat attcagttcg aacttttaat acacgaggaa caacatagat gcgtaggtca
       61 acccccttga ccctgttact ggtttattcg ctcctgttat caacgacggg cgcgctgccg
      121 ctccccgcga cgcacgcgca agtctccgag cgtcgcggca actcgctgcc cggtaagatg
      181 tccgactcgc tgcgcaggcg tgcgaaggct tcgcggcaga gcaacaaaga cggcgacggc
      241 ggcgagcgtg cgcgcgtcat tctcaatctg gccggagact ctgcggcggt caagcaatcg
      301 cgcgcgctgt tgcagggcgc gggcgcaaac gtccggcagg agttgaacga actcggcatg
      361 atggtggcgg acgtgcccgt cgaacgactc gaagaactcg cagcgcacag cgaagtcgca
      421 tggatgtcgg aagacgcaga ggtgcggacg cttgcgacga ccgacaacac gagccatctt
      481 gagttgacga ccggcgcgag caaagttttg ccgtcggatg tgacgaaggg cggcgcgggc
      541 aacggcgtcg gcatcgcctt tctcgatagc ggcatcagcc gcccggacgc agcggagttt
      601 gcgggctaca cgctcgacac gagcggcggc ctgctcggcg gcggcggcct cctcggctcc
      661 ggtctgctct ccaccccgac gcttacgccc tacaaccgca tcacaaagga gatggacttc
      721 accggcgaag gcacgaagct cgacaactac ggacatggca cgcacgccgc cggagtcgcg
      781 gcgggcacgg gtcaggcgag cgaagagtac gcggcgagcc acgcgggcgc gcccacttac
      841 ggcggcatcg cgccgatggc gaacctgatc agcgtgcgcg tgctcaattc gcagggcgtc
      901 ggcacagtct ccggcgtcat cgccgggatc aactgggtca tccagaacaa gtcaacctac
      961 aaaatccgcg tgatgaatct ctcgctcggc acttcgatca cgcagagcta caagaccgac
     1021 ccgctctgtc aggcggtcgg gcgcgcggtt gacgcaggca tcgtcgtggt tgttgcggcg
     1081 ggcaacctcg gcaaagattc caacgggcag acgatctacg gcggcattct ctccccggcc
     1141 aattcgccga aggtcatcac cgtcggcgcg accaacacgc aggataccgt caagcgttcg
     1201 gacgatgtgg tgacgaccta ctcttcgcgt ggtccaacgc tcgttgacgg actcgtcaag
     1261 cccgacctcg tcgcgcccgg caaccgcatc ggcgcggcgg aatcgtccgt cacgtctccc
     1321 acggcgagca gttcttattc gacgagcggc ggcgggggat tgctcggcgg tctggtctta
     1381 ggcagcccga ccacgacgaa gccgattgcg accggcacgt atcgcgtctg gagcggcacc
     1441 tctttctccg cgcccgccgt ctccggcacg gtcgcgctga tgctcgaagc caatccctcg
     1501 ctcacgccgc gcatcgtcaa agcactcctg atgcgcacgg cgcaacgcct gccctactac
     1561 gaaacgagcg tgatgacagg gcagatgtcc gcctacgagc gcatcatcac ggaaggcgcg
     1621 ggcgaactga acgccaacgc tgccgtcgcg ctcgcacgcg ccgtccgccg cgacgccaac
     1681 ctcgcgcgga cgggcgagaa tttgatcacc aactcgaagc tcacttacgc caccgtcccc
     1741 ggcatcactt cccctatcgg cggtgaggcc atcccgctca acaacggcct cgtctggtca
     1801 gacggcgttg ccttcaacag tcgcctgcaa ctgacaaacg gctaccgcct ctcagacggg
     1861 cgcacgatca cgagcggctt cgccatcgcc gacggctacc tgaacgcaga cggaagctac
     1921 gtgctcggca acggcatcgt cgtcgccagc ggcattgtcg tggcgagcgg catcgtggta
     1981 gcgagcggca tcgtcgtggc gagcggcatc gtggtggcga gcggcatcgt cgtcgccagc
     2041 gagtatgtgc tggccgacgg gtcgttttcc acgggcgcgc tgaacggctg tagttttaac
     2101 tacgaggccg tctgggcgtc gaatctggtt gatccgggca cggtggacat caagccagac
     2161 caagtgatcc tgctcggcga ggacgcggtt cccgtcggcg tgcggcgtct ggcaaacgtc
     2221 agcccgctct acccgcgccg ctaggagaat cgagggacgt gcaggcaggc gcgcttgctt
     2281 gcacgtcctt caacctgccg ccctcgactc gcctctctta aacccgcttc tctgcaactc
     2341 gctgcctgtc aaacctgaaa gtgcgcacca tgacgccgga caacgccaaa gtaaaaagca
     2401 acgatctcta cacgacactg ctcaccgtcg tcagtctcgc cttgtggctc gcggcgagcg
     2461 taaccttttt tgtcgagcat gcgtggcggg aacaactggc ggtgctcgcg ctcttgccgc
     2521 tcgtcatcgt gacgggcatg ttccccaaca ccctgccgct gccctccgcc ctcaagctct
     2581 cggaagagcg catgaacttt acgctctcgg acgcgctcgt acttttgatc gcctgtcgtt
     2641 acggcttcgc cgcctgcgtc tttgtggcgg gcatcgaagg actcacttcg tcgagacgcg
     2701 tcgttcgtcg tctctccagc aatctctatt cgttcggcat gatgtcgctc gcggcggcgg
     2761 cggcggcggc gagcctccat gccgtgctgc gctacggttt cggcgaagct gccggtacgg
     2821 agcacgcttt tcccgctatc gccgtcgccg tgctcgcggc gagcatcgtg cacatcagcg
     2881 tcaacacggg cgtcatctcg ctgctctttg cgacgcgtct cggcgtcccg gtcatgtcga
     2941 gttggaagga gaacttccgt ctggccgtgc cgctctttct gccgacgagt gcggcagcga
     3001 gcttgatgta catcgggctg caatacaacg cgctcacgat ggtcgccatc ggcgcgccgc
     3061 tcctcttcgc catctacttc gggcaccgac gctaccgcga cagcatgcag gagcaactca
     3121 atctcatcga gcgcgcacag caggaacgca tctcgacgct cgaacaggcg caccgcgaaa
     3181 ccatcgaagc gctcgccgtc acgatcaatg cgaaggacga ggtgacgcac gaacacgtcc
     3241 tgcgcgtgca gatttacgcc gccggggtgg cgcgcctgct cggctgctca gatgccgaga
     3301 tcgaagccct gcgcgcgggc gcgctcctgc acgacatcgg caagatcgcc gtgcccgatt
     3361 acatcatcaa gaaacccggc aagctcaccg ccgccgagtt tgagaagatg aagattcaca
     3421 cgctcgtcgg cgcgcagata ctcggtcgcg tcggcttctc atacccggtc gtgcccgtcg
     3481 tgcgctcgca tcacgagcgt tgggacggca aaggctaccc cgacggcctc gcgggcgata
     3541 gcatcccgct cacggcgcgc atcctttcgg tggtcgattg cttcgacgcc gtgcgcgaag
     3601 accggcagta tcgcaaaggc atgacgcgca cggaggccat agactacctg atgggtcaga
     3661 gcgggacgca gtacgacccg cgcgtcgtcg gaactttcat cacgcacctg cccgagttcg
     3721 aggccgagat cgcggcgcac cgcaacacga ccgcgccgac ctacggcatc gaacccgtcg
     3781 agcaactatc ggaggccgcg cgccgcgtcg cccccgccgc aggtctggcc gaagcgtctg
     3841 ccgcgcccca cccacagacg gcggaggtcg agttcacaga ggccgagcgc gacgcgcttt
     3901 cacacgccgc ccgccgcttg agcgaggcgc gcgatacgga tgaagttatc gcttcattca
     3961 tcgagagtgt gaaaacgctc gtgccttacg attcatgcgc gttgacgctc gccccgccgc
     4021 acacggacga gtcgcacatg gcctacgcgg acgggcgaca cgcagacctc ttgaacaatt
     4081 tccgcatcat ccccggcgaa ggcgtgacgg gctgggtgct cgccaatcgt caaccgttct
     4141 acaacgctga tcccttgctc gaccttccgc ccgcgctcgc ggcgcgcgac gattcctacc
     4201 gcacgctcgc cgtctgcccc atcctgcgcg gcgtcgagct tcacggcgtc gtcacgctct
     4261 actccgcaac gctgcgaact tacgaacccg ctcaacagca actcatcgaa gcgttggcct
     4321 cgctgctcgc cgcgaggctc tccgccaacg ctcaagtcgc atcgcccacg gttcttgaaa
     4381 tctcgccgcc cgctaccgct catctatcgc agggcgagtt ggctttcaat atgccgccgc
     4441 cgcaccttgc gggcgttgcg ctcgaatccg agttggcgca ctagtcgctt cgatacgtta
     4501 gacgccagcg caacgctgca aagagaaagt caccgccgcc gactccaccg actccacgca
     4561 cagactcgac acgcttcccg ccacgcattt tccctccgcc gctttcaagc cgactgcccg
     4621 cgtactacta tcggggcggc gcacgcatct gatatgtgtg cgtcgtccgt acacggcggc
     4681 atccggccac ggcgagctat gccgtgcgag gcgatttcgt tcacgagacg ggaggattcg
     4741 cgttgagaga attgaaacag acgggcatcg acaacaacgc cgtcgccacg cgcacggcga
     4801 cgggtacgct cacgcctgag aggcggcaca cgcacgtcga gatcgagcag agcctcgacc
     4861 aactgagtcg ctggatggac gggctttttc gcgttccggg cacgagttgg cgtttcggcc
     4921 tcgacgccat cgtcggcctc attcccggcg tcggcgacac ggtcaccacc atcgtcggct
     4981 tctacatcct cgccgcggga gtgcgctacc gcgtctcgaa agcgacgctg ctgcgcatgg
     5041 gactcaacat cggcgtcgat tacatcttcg gcgcgatccc tgtcctcggc gatctcttcg
     5101 acaccgcctg gaaatcgaat caaaagaacg tcgaactgct gcgccgccgc gccaccgtca
     5161 cggcagagga agcgcgctcc ggacgactga gcgactggct cttcgtcggc ctcgtcatgc
     5221 tctgcctgct cgcgctcctc gtcggctcga tcacgatcct ctggttcatc ctgcgcttcc
     5281 tctcagacca gttctccgcg ctcttttaaa ctgaacagaa acggcgcgac tcgaaaacga
     5341 agaaggcgat cccactggcg ggatcgcctt cttcgttcta acacgtctgt cgcttgtgcg
     5401 ctccgaactg ctcaggaggc cgcctgcccc gtgtgctctg cgagcacgcg ttcgagcaca
     5461 gccgcgacct cttccaaacc ctgctcgccc gacgcgaagc tgcgcaattt tccctcgccg
     5521 tcgaacaggt aataggcggg cacgtcgccc tgctcgttct gaaacgcatc gcgcaacttg
     5581 tattcgttgt ccaccgcgca cggctccgtg atgttgcaga gcgcgacgcc ctcgcgcacc
     5641 gtctccgtat ccgcctcggc ctcctcgcgc ggcgtgtgca cggcgatgac gcgcaagccg
     5701 tcagccgcgc gctcgtcgcg ccaagccgcc acgcgcgcca tgttcccttt gcaggcgtcg
     5761 caattgacag accagaagtg aaccagcgtc ggctgtccct tcgcctccgc ctcggcgcgc
     5821 gcgcccgtcg cattgaacca ctcggtcgcg ccttccagcg aaggcatgtc cgtcccgatc
     5881 ctcggcataa ctcacccctc taaaacagtg acaagtgaca ggtgacgagt gacaagttaa
     5941 aaccatgagc ggctttcggc tgcccgctgt cacatctctt gttacttgtc actgctttca
     6001 aacttgtcac ttgtcactcg tcactgcttc cattactgct ttcattctaa cgctcgctaa
     6061 aggtggccgc aaagaggccg tcggcttcac agggtcggat ggcgcgcgac gtaatcgacg
     6121 agcgcgcgga cgtaggaggg aaacggcggg caggcgatat ggtgcggcgc gagcaactcg
     6181 acggcgcggc tcgtatcgta agtttgcttg atgaagaaat agggcacgcc gatgtgcggc
     6241 agcttcgtca acttctcgga gaacgggagg ccgagcgtcg cgcgcacgag cggcttcggc
     6301 agttgcacgc gcgaaccgcg ccccacgagc gcgtgcgcga tggtttcaaa aagctcgctc
     6361 gtggtgagcg gttgcgggtc tgcgagttgg acggtcgcgc ccaccgcgct cgcgtcgcgc
     6421 gccagtgccg ccatcgcttc gaccacgaaa tcaaccggca cgatgttcag acgcacatcc
     6481 ctgtttccga tgttcaccag agagaaaagc cccggccaga tgcgcaggta cttgatcagg
     6541 taatagacgc cgtcgtactt cgccgtctcg cccgaacgcg aatcgccgca caccaccgcc
     6601 ggacgatgaa tcgtcacagg caactcgccc ttcaatcgtt cgacttccaa ctcggcgaga
     6661 tatttcgtct cctcgtaaaa attgcggaag cccgccgtgt gtgccaactc gtcttcgcga
     6721 atcagcccct cgcgcaagcc cgcgacgtag caggtcgaaa cgtaatgata acgcgtgaga
     6781 tgtttgcacg cgcgcgcaaa ctcgttgacg tggcgcgtgc cctccacgtt cacacgcatc
     6841 gcaggctcgc gccgcacggc cagatcgtaa atcgccgcca ggtgaaacaa cacgttcgtc
     6901 tccgcagacg cccgcgcaaa ctcggcttcg ctcatcccca gccgcgcctc gctcgtgatg
     6961 tcgccttcaa cgatccgaaa acgctccgcg ctcgcgcccg tctccgtggc cagccgcgcc
     7021 acctcttcgc gcgcacgcga caggaaagcg ggctgcacca gcaggacgaa gcgcgtggag
     7081 gaagcgtcgc gcgccagttc cttgatcaaa cgcgcggcga tgaagccggg gaaacctgtg
     7141 agaaaaattg tttcttgaga taacataaaa agcactcatc acggagacac agaggagacg
     7201 ggcgcacgaa agtttctgtg tgtggcgcgg cttgtgtcgg gcgagtataa ttcaagcact
     7261 ttggcataac ctaactgccc tctgcctgtc cggcgcgaga tgcgcttccg tgcccttcgt
     7321 cgccggtgca ggagaaaagt taagtcccga atttattgtt agcgttcaag gagaagaaga
     7381 gttcgcatgc aatttccccg tcctctcgtc gtccggcgac acacgctgct cgcactcgcg
     7441 ctgacgatag tttccgccgt ctcgacctac gcgcaggcca acgcgcaggc caatgtgcag
     7501 gcgaccgcga cgccgtcgcc ccaagccggg gagacggtgc gcgtgacgct cctgcaagtc
     7561 aacgacgtat accagatttc gcctgtagat cgcggcaggc gcggcggcct cgcgcgcctc
     7621 gccacgctcc ggaaaatagt gttggaggaa tcgccgcaca cgctctacct gctcgcgggc
     7681 gacacgctcg cgccttcggt cgcttcgaac atcttcaaag gccgacagat gatcgccgcc
     7741 tggaacgcca ccggactcga ctatgccgcg ctcggcaatc acgagttcga cttcggcgat
     7801 gcagtcctgc gcgagcgcat gcgcgaatcg aagttcacat ggctcggctc gaacgtgttc
     7861 gaccgcaaga ccggcaaact cttcggcgac atgccgccct tcgtcgtgcg cgaattcggc
     7921 ggcgtcaaag tcggcctctt cgggctgctc acagtggaca cgaagcagac ctcttcgccc
     7981 ggtgcggacg tggagtttcg cgacccttgc cgcacggcca atgagacgat caaacaactc
     8041 cgccaacgcg gcgtgcgcac catcgtcgcc atcacgcacc tgaccatcga gaacgacaag
     8101 cggctcgcgc gctgcgctcc cggcatcgac gtgatcatcg gcggccacga acattccgtg
     8161 ctgcaatcgc tttcggagcg cacgccgatc ttcaagatgg gatcggacgg gcgcaatctc
     8221 ggacgcatcg atctcaacat ctatcgccgc aacggcacgg tggagagcgt cgattgggag
     8281 atcatcccgg tgacggacgg ggtggaggaa gagccgaagt cggcagccat catcgccgaa
     8341 tatgagaaga agctgtcagc cgaactcgac ctgcccgtcg gtcgcaccac ggtcgaactc
     8401 gacgcgcggc aggagacgaa ccgctcgcgc gaaacgaacc tcggttcgtt catcgccgac
     8461 tcgtatcgta agtacgcgaa agccgacgtg gcgattgtca acggcggctc gattcgttcg
     8521 aacacaactt atggtcccgg cacgatcacc aagcgcgacg tgctctcgat cctcccgttc
     8581 gagaatccga ttgtgaaagt cgaggtcacg ggcgcgacgc tgcgtgccgc gctcgaacac
     8641 ggcgtgagcc ggattcgaga agagaaagag gcgggactct ttccgcaagt ctcaggtctg
     8701 cgtttcactt acgacgggcg gctcgccccc ggcgcgcggg ttaagagcgt gacggtgaac
     8761 ggcgcgccgc tcgacaacgg caagacctac acgctcgccg tcaacacata cgtgctcggc
     8821 ggcggcgacg gctacacgat gttcaaagac gccccccttc tgctcaagcc cgaagaggcg
     8881 caggttgaac ccgccatgct catgaacatc atcgccgccg ccgcagagat cgccccggcc
     8941 actgacgggc gcatcgagcg cagtgacaag tgacgagacg gaagtgatga gtgatgagtg
     9001 atgagtgatg agtgatgagt aatgagtgat gagtgataaa gttgatgagc gcacagccgg
     9061 aaactgttcc tgcttttaac tcgtcactcg tcacttgtca ctcgtcactg aattattatg
     9121 tttgaagaag atactttatt gagtggcgcg aagagcgagg ccgtggagtt gttccggcgc
     9181 gcgtatgagg cgcagcagtc gcaggcttac gcggaggcca tcgagttgta ccgccgttcc
     9241 atcgaaacct atccgaccgc cgaggcgcat acctttctcg gttgggctta tagttttctg
     9301 gaacgctacg acgaagcgat tgacgcctgc ctcgaagcca ttcgcgtcga tccggccttc
     9361 ggcaatcctt acaacgacat cgggagttat ctcatcgcca aaggcgatct ctacaactgc
     9421 gtacgctggt tcaagcgcgc cctcgaagcg gcgcgttatg agtcctacgc gttcccgcat
     9481 ttcaatctgg ggcgcgtcta cgagcagcgc ggccacctgc tcaaagccgc gcgccattac
     9541 ggcctcgcac tcaagcaagc cccggacttt gcgcaggcca ccaaagctct ccgccgcctg
     9601 caagcccggc tcaattgatc tcccgcccct ctcgcaccac acacgaaaga aagtcgcagc
     9661 gacatgaaca cgtacctcct gacctggagt ccgaagaagt ggcaatggac tgacctctca
     9721 caacggatta gagagatcac ggagagagga ttctctctca ccgattggag ttgcgggaac
     9781 aacaaatcaa tcgcccgtgg agacaggctg ttcctgctgc gtcagggcga agagccgaga
     9841 ggaatcgtcg gggcggggtt cgccgcctca gagccttatg cggagattca ctggcgcgaa
     9901 gagaaagcgc gggtcggcaa gactaccatg tacgtaaaag ttcgttggga gattctgttg
     9961 aaccccgaaa gcgaatccat ctttccccgc gagtggttga atgaaggctt cctgtcaaaa
    10021 gtaaactgga acacacaaat atccggcatc agcatccgta gcgaggcggc ggatattctc
    10081 gaagagaggt gggaggcatt tttggaaagc agggaaaacg agttctctct tttcagcaat
    10141 gcttcgccgg actagccgga cgcctcttcg ccagtcggca accggaagag actgcttgtt
    10201 cataaccggc tacacacggg gcgtcactcc gtctcgtgcg gctcaatgcc cgacacaatg
    10261 cccgacataa tgcccgacaa gtcgcccttg aagagttcgc ggttgagccg catcttgcgg
    10321 cggttggcac ccacggagac ataaaccacc ccggcctccg ccacttcaat gcgcacggag
    10381 atttcgcttt aacgtccggc ttcggcgatt cgataattca gctacacgct ccgcaacatt
    10441 cgaccttcat ttttgtgcct gctgccgatt actctggcgg atgcaaccga atggggatag
    10501 aatgtgccag ccttctagtt ctcataatgt tctgccccgc gccgcaactc atcactcgtt
    10561 gctagaagaa gctcatctct cctcgtcaga aagatgcctc tcactacagg tacacaactc
    10621 ggacgctacg aaatctgctc gctactgggc atgggcggca tgggcgaagt gtacttagct
    10681 tacgactcac ggctgaggcg caagatcgcg ctcaaagttc tacccgatag cttgattgac
    10741 agccccgaac atctacgccg tttcgagcgg gaagcgagag ccgtatctgc cttaaatcat
    10801 ccgaacatta ttaccatcca tgaaataggc acggacggcg acacgcactt tatcgccatg
    10861 gaactcattg aaggcgagac gctgcgccag aagttgcgaa cggggcgttt agagattgaa
    10921 cagacactcg atgtcgtcac acagatcgcc tctgcgctca atgccgcaca tggaagcggt
    10981 attgtgcacc gcgacttgaa gccggaaaac atcatgctgc gcgaagacgg tctggtgaaa
    11041 gtgttggatt tcggtttggc gaagctggtg gagaagcgag gcgcactgcc cggcgacacg
    11101 caagccacga ccctcgccat cggactgaca cagcccggcg cagtgatcgg aactgtcgca
    11161 tacatgtcgc cggaacaggc cagagggcaa gaggttgatg cgcgaaccga tgtttttagt
    11221 ttgggcttgg tgtggcatga aatgctgtcg gggagcataa cgtttgcagg cgcgacggcg
    11281 agcgatatga ttgccgccat cttgaaatcc gagcctccgc cgctgagcca tttcaatcaa
    11341 aacattcccg cggaacttga aagaatagtc ggcaaaagtt tagccaaaga ccgtgaagag
    11401 cgttatcaaa ccgcaaaaga cttgctggtt gacctccgac ggttgctcaa aaaacttgag
    11461 actcagaatg aaatcgggcg cacctcgccg ccagacaaaa cagagcatga aaacgccacg
    11521 caaatcctcc cggctcgtcc ggctcggagc ggcgacaagg ttagcggcgc aagccgaaca
    11581 tacagactcg gtttcgccat tgcctcaggg gttttattgc tcgcgtgcgt ggtttggggc
    11641 tactggttta ttgccggtcg cacgtcacag ttggggcaga tcgaatcaat tgctgtcctg
    11701 ccgtttatta acgagagcgg caatagcgat gtcgagcatc tttcggacgg gatgaccgaa
    11761 acgctcatta acagcatctc taaactacct cttttatcag tcaaagctcg tagctccgtg
    11821 tttcactaca aaggcaaaga gattaagccg gaaagcatcg cttccgagtt gtccgtgcaa
    11881 gcactcttga ccgggcgatt tgtccaacgc ggcaatgatt tgacactgta cttagcgttg
    11941 gttgatggac gcaatggtaa tcagctgtgg ggtgagcagt acaatcgcaa gctggtggac
    12001 ttagtgctct tgcagagcga gattgcgcgc gatgtctcgc ggaaattgcg tgcgcgttta
    12061 tcgagagcag acgagcaaga ggtggtgaga aacgacacgg acaacccgga agcctacgag
    12121 ctatatctca aaggacgtta ccacgttttc aaactcacac cgccggaagt ggataagggc
    12181 atctcatatc tccaaaaggc aattcacctt gacccgaact atgcgctcgc ctatgtcgga
    12241 ctctctcagg catatcgtgc gcgcgtgctc ggcggcgaaa tgaatccggc tgaaaccttg
    12301 ccgaaagcga aagaggcggc acaaaaggca atccggatta aggacgctct ggccgaagcc
    12361 catggtgccc ttgctaatat gacgttctgg tacgattgga attggaacg
//
LOCUS       DASWNS010000081         7629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_211453, whole genome shotgun sequence.
ACCESSION   DASWNS010000081 DASWNS010000000
VERSION     DASWNS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211453"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VGB76_02790"
     CDS             complement(<1..518)
                     /locus_tag="VGB76_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097858.1"
                     /translation="MLHKISTSTRLSLRALPALALSLLLTALPLPLVAQTPAQPQQGM
                     GGNATGEARSYASKRTVNITDPKAPVVYEDVTAKTALANFRHRAGGAAKDYILDTPAG
                     GLAIFDYHTDTRPDIYLLNGSTFAALAGKERAPRAALYRNLGNWKFEDVTARAGLENE
                     RWGMGVAAGDYD"
     gene            complement(696..1793)
                     /locus_tag="VGB76_02795"
     CDS             complement(696..1793)
                     /locus_tag="VGB76_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097859.1"
                     /translation="MCRKDRRRLLFALSVCLVWAGSLHAQTPKPKPVAVKFDEFKYDF
                     KEDMKARLAPLAGALKQQPRARAFIIGYAARMFRYGDSSASDIAAYVRLDLTNRHEDT
                     IDWDRIVLIDGGYREEQMVELYLVPPGAAPPAPRPTLQPAQVTFCPHIMVNPPFFVWD
                     VKGTLKFSATVREELTKIVPTYLWTVSHGRISSGQGTAEIAVQQSAEEYQPVTATVEI
                     GGYAPGCGSSVSASATSPEKLSHVPLKFGEMGNVAAGDMKAHLDNYAVGLAAAPEMQA
                     YIIFYGGRRDNGRLPRRGEAERLAARLPNYLVKTRAIAPARITLINGGFREEWTAELW
                     LSPRGAKAPAPAPTIAASEIKFRPERRRRRR"
     gene            complement(1817..3697)
                     /locus_tag="VGB76_02800"
     CDS             complement(1817..3697)
                     /locus_tag="VGB76_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEY0097860.1"
                     /translation="MIHQWCSKFQMNANSSSLVRALFSSNSLAFLLAFSLLSVAVRPA
                     YAQTQPPAPQPTPTQQRTGRSYSGVDPLKTPPPPAPQSPSPVTFTDITAATRINFKHH
                     ASVTSRKYLLEAMGAGVALFDYDNDGRLDIYFTNGARLDDPMPAANAQPDKRDPRFWN
                     RLYQQRPDGTFADVTERAGVAGGGYSMGAAAGDFDADGFTDLYVTAYGANILYRNRGN
                     GTFEDVSKKAGVAGGGWSTSAGWFDYDNDGRLDLFVARYVDWDFARGDLYCGEPRPGL
                     RAYCHPDNFKGATNLLYRQKPDGTFADVSAVAGIADPNGKGLGVAFADFDADNFTDII
                     VANDSVRQSLYHNKGDGTFADVALIAGVGYDENGKTFAGMGVDAADFDNDGRPDIFIT
                     ALSNETYPLYQNTGEMSFNYVTQTSGVGQITLLYSGWGTRFIDFDNDGWRDIFVAQGH
                     VLDTIELTTSYLKHRQTPLLMRGVAAAAKPGKPHTFVNVSATAGASFNQPYAARGAGF
                     GDLDNDGDTDIVLGVADGAPVIMRNDGTKNHWLGLVLSGTRSHRSGTGARVVVTDSTG
                     RRQTFDATNSGSYLSSSDPRLLSGLGAATAVQSIDIRWPSGQKQTINRPEIDRYLTIK
                     EP"
     gene            3802..5676
                     /locus_tag="VGB76_02805"
     CDS             3802..5676
                     /locus_tag="VGB76_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0097861.1"
                     /translation="MRRLKFPPTPLLQPKFYALCGLMFALALVCFVVRSTHAQQAATP
                     AAPAAPAEGRARFEVVEDLSESLANDLLELSVATRDRDLKRTSEFFPAELTAKPFTSH
                     PEVTETKVKWIGAHNWTLRPPAGNATPHHVAAPPTVSTVAKTNLPKPTPNPDPLVQKI
                     SRERFMADWSHFLAHFSEIEDARFKVKEAYFDADAQAVAGAKEPTAVVGAKGKARVAF
                     YVIGRDLDGRREWARGVANVNARYDASNRWQFDSFLLVSLDSLVATSDLFSEVAIPAG
                     LGASLPAFGSPGNGGFVWHGAAASDFNQDGAIDLFVTAPARNFLYLNDGKGRFTDASD
                     AAGVRLLAAGVAPLVFDYDNDGDGDVFVSNVGAQVLFENRLVPDGKLEFRDVSQEAGV
                     GGVQAIGFSAVAGDVNNDGRQDIYVTSYNRYGQITPDSWFRATNGTPNLLFINQGNGT
                     FKEEAKRWGVDDSRWSYAAAFADVNADGRVDLYVANDFGEKAMFINRGDRFTDEAKER
                     GVLDPGNGMGVSFGDYNNDGLLDVHASNMSSTAGNRILARLFPNQSPKENVLKKLAAG
                     NNLFENQGDGKFKDVTAEVGGFGGGWAWGGGFIDFDNDGWEDIYTPNGFISGKSMKDT
                     "
     gene            5698..6864
                     /locus_tag="VGB76_02810"
     CDS             5698..6864
                     /locus_tag="VGB76_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HEY0097862.1"
                     /translation="MTQKNDGKDEKGALRVDQTQLMFGEGFSFSGYERDPLYLNLGTK
                     KFMDISGVSGIDSITDGRAGVFADFDNDGDLDVFSTTIQGQAHLLFRNNVGQDGNHLR
                     IALEGNERTGRDAFSAVVRVKTSQGTLTKIKDGGAGFISQHDPRLLFGLGRDERAEWI
                     EVTWPHGKVERFAGEARAGTSLLLREGTGRAQELTLARAKLPDPLTRAEAFARGLKIK
                     IGQPLPTLALKSLDSVAAAGRSASRPGAYLRPGRRTLINVWATWCTPCAAEMPELEKL
                     RAGLNTRGIDLVGLNVDSEPKADVRGFLREHRVGYPNFLGGVAAIESLYATDELSVPM
                     SIVVDGRGIVTEIIPGWSAETQRRFRELAGEGAGSGGSSGAPAAKTKARSLPRR"
     gene            7015..7524
                     /locus_tag="VGB76_02815"
     CDS             7015..7524
                     /locus_tag="VGB76_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097863.1"
                     /translation="MNRNVSGRLAVLFAVLTFALIPAGVNAQVIITGAQLSRIVPAGF
                     YFEGQSAPTQMRNASAARFGAKQHLVVGMVDTSGYSADVRSRYEGFFIIDTPMMVGGQ
                     ELGIGAYGFGFVENNRFTISDVAGNQIFTVNTEPDRNLRRPRPLVLTRGAGGVRLYSG
                     RRYVAITLR"
BASE COUNT         1397 a         2309 c         2487 g         1436 t
ORIGIN      
        1 ttgtcgtaat caccggcggc gacgcccatt ccccagcgct cgttctccaa gccggcgcgg
       61 gcggtcacgt cctcgaattt ccagttgccg agattgcgat agagcgcggc gcgcggggcg
      121 cgttctttgc ccgcgagcgc ggcgaaggtc gagccgttga ggagatagat gtcggggcgc
      181 gtgtcggtgt ggtaatcgaa gatggcgagg ccgcccgcag gcgtgtcgag gatgtaatct
      241 ttcgccgcgc cgcccgcgcg atgacgaaaa ttggcgagcg cggttttggc cgtcacatct
      301 tcgtagacga cgggggcttt cgggtcggtg atgttcaccg tgcgcttcga ggcgtagctg
      361 cgcgcttcgc ccgtggcgtt gccgcccatg ccttgctgcg gctgcgcggg cgtctgcgcg
      421 acgagcggga gagggagcgc agtgagcagt aaggagagcg cgagcgcggg aagcgcgcgt
      481 agggagagac gtgtggacgt tgagatttta tgaagcatga atcttatctt ttcgacagaa
      541 cggaaaattg aagcattgaa gcgcggcttt cgaggtcagc atgtgaagag acaaaataac
      601 gagtcgattt gtcgcctgtc aaagcgcgaa ggctcgcgcc gggatggctg agcgggcatt
      661 gcgtccgagc cgcaggcgac gcgaagagtt tacgttcatc tacgccttct tcttctttca
      721 ggacgaaact tgatctcgga tgccgctatc gtcggcgcgg gagccggagc tttcgcgccg
      781 cgtgggctga gccataattc tgccgtccat tcctcgcgga agccgccgtt gattagcgtg
      841 atgcgtgcgg gcgcgatggc gcgcgtcttc accagataat tcgggaggcg cgcggccagt
      901 ctttcggctt cgccgcgtcg tgggaggcgg ccattgtcgc ggcgtccgcc gtaaaagatg
      961 atataagcct gcatctccgg ggcggctgcg aggccaaccg cataattatc gaggtgcgcc
     1021 ttcatgtcgc ccgccgccac gttgcccatc tcgccaaact tcaaaggcac gtggctgagt
     1081 ttttccggcg atgtggccga agcggaaacg gaactgccgc acccgggcgc gtagccgccg
     1141 atctcgaccg tcgccgttac cggctggtat tcctccgcgc tctgttggac ggcgatctca
     1201 gccgtcccct gcccgctgct gatccgtccg tgggaaactg tccagagata cgtgggcaca
     1261 attttcgtca actcctccct gaccgtcgcc gagaatttga gcgtcccttt aacgtcccag
     1321 acaaaaaacg gcgggttcac cataatgtgc gggcaaaagg tgacttgcgc gggctgtaag
     1381 gtcgggcgcg gcgcgggcgg cgcggcaccc ggcggcacga gatacagttc gaccatctgc
     1441 tcttcgcggt agcccccgtc aatcaggacg atgcgatccc aatcaatagt atcttcatga
     1501 cgattggtga gatcgagccg cacgtaagca gcaatatcac tcgcgctgga atcgccgtaa
     1561 cgaaacatgc gtgcagcgta accgatgatg aaagcacgcg cgcgcggttg ctgcttcaag
     1621 gctccggcga gaggggcgag ccgtgccttc atgtcctcct tgaaatcgta tttgaactcg
     1681 tcgaacttga cggccacggg cttcggcttc ggcgtttgcg cgtgcagaga acccgcccac
     1741 acgaggcaga cgcttaaggc gaataacaga cgccgccggt ctttgcgaca catgctcgtt
     1801 ctccacttac gactcatcaa ggctccttga tcgtcaggta acggtcgatc tccggcctgt
     1861 tgatggtctg cttctgcccg ctcggccagc gaatgtcaat tgactgcact gcggtcgccg
     1921 cgccgaggcc ggagaggagg cgcgggtcgc tcgaagagag atagctgccg gagttcgtcg
     1981 cgtcgaaagt ctgcctgcgc ccggtcgagt ccgtcacgac gacgcgtgcg cccgtgccgc
     2041 tgcgatgcga tctcgtgccg gagaggacga ggccgagcca gtggtttttc gtcccgtcgt
     2101 tgcgcatgat gacgggcgcg ccgtccgcga cgccgagaac gatgtccgtg tcgccgtcgt
     2161 tgtcgagatc accgaagccc gcgccgcgcg ccgcgtatgg ttgattgaaa ctcgcgcccg
     2221 ccgtggccga gacgttgacg aacgtgtgcg gcttgcccgg ctttgcagcg gcggcgactc
     2281 cgcgcatcag gagcggcgtc tgcctgtgct tgagatagct cgtcgtcagt tcaatcgtgt
     2341 cgaggacgtg gccttgtgcg acgaagatgt cgcgccagcc gtcgttgtcg aagtctatga
     2401 agcgcgtgcc ccagccggaa tagagcagcg tgatctggcc gacgccggag gtttgcgtca
     2461 cgtagttgaa actcatctcg cccgtgttct gatagagcgg gtaggtttcg ttcgagaggg
     2521 ctgtgatgaa gatgtcgggt cgcccgtcgt tgtcgaagtc ggcggcatcc acgcccatcc
     2581 cggcgaaggt cttgccgttc tcgtcgtagc cgacgcccgc gatgagcgca acgtctgcaa
     2641 acgtcccgtc gcccttgttg tgatagagcg actggcgcac gctgtcgttg gcgacgatga
     2701 tgtccgtgaa gttgtctgcg tcgaaatcgg cgaaggcgac gcccagacct ttgccgttcg
     2761 ggtcggcgat tccggccacc gcgctcacgt ctgcaaaagt gccgtcgggc ttctggcgat
     2821 agagaagatt ggtcgcgcct ttgaagttgt cggggtggca ataggcgcgc aggccggggc
     2881 gcggctcgcc gcaatagaga tcgccgcgcg cgaagtccca atccacgtag cgcgcgacga
     2941 agagatcgag ccgcccgtcg ttgtcgtagt cgaaccagcc cgcgctcgtt gaccagccgc
     3001 cgcccgccac gcccgctttt ttcgacacgt cttcaaacgt cccgttgccg cgattgcgat
     3061 agagaatgtt tgcgccgtaa gcggtgacgt agaggtctgt gaatccgtcc gcgtcaaagt
     3121 cgcccgccgc cgcgcccatg ctgtagccgc cgcccgccac ccctgcgcgc tcggtcacgt
     3181 ctgcaaacgt gccgtcgggt ctttgctggt agaggcgatt ccagaaacgc ggatcgcgtt
     3241 tgtcgggttg tgcgttggcg gcgggcatcg gatcgtcgag gcgcgcgccg ttggtgaaat
     3301 agatgtcgag ccgcccgtcg ttgtcgtaat cgaagagggc gacgcccgcg cccatcgctt
     3361 ccaacaggta cttgcgcgag gtgacggagg cgtgatgctt gaagttgatg cgcgtcgcgg
     3421 cggtgatgtc cgtgaaggtg acgggcgagg gcgattgggg cgcgggcggc gggggcgtct
     3481 tcagaggatc aacgccgctg taggaacgcc ccgtgcgttg ctgcgtcggg gtcggttgcg
     3541 gcgcgggcgg ctgcgtctgc gcgtacgcgg ggcgcacggc gaccgacaac agcgagaagg
     3601 cgagcaggaa cgcgagcgag ttggacgaaa aaagagcgcg gacgagggaa cttgagttgg
     3661 cgttcatctg aaacttcgag caccattgat gaatcataaa tcgttcgcgc gggagacacg
     3721 gaagcgcgtg cggacgcgct tggaagcgta ggctcacttt acagtcaaat gaggtgcata
     3781 gtacagtgac gcgcgaaact tatgagacgc ttgaaatttc ctcccacgcc cctcctacaa
     3841 cctaaatttt acgcgctgtg cggcctgatg ttcgcgctcg cactcgtttg tttcgtcgtg
     3901 cgttcgacac acgcgcaaca ggcggcgacc cccgccgcgc ccgctgcgcc agccgaggga
     3961 cgcgcgcgtt tcgaagtcgt cgaagacttg tccgagtcgc tcgccaacga tctgctcgaa
     4021 ctgtccgtcg ccacgcgcga ccgcgacctc aaacgcacgt ccgagttctt ccccgccgaa
     4081 ctcactgcca agcccttcac ctcgcatccc gaagtcaccg agacgaaggt gaagtggatc
     4141 ggcgcgcaca actggactct tcgcccgccc gcaggcaatg cgacgccgca tcacgtcgca
     4201 gcccctccca ccgtttcgac agtggcgaag accaacctcc cgaagccgac gcccaacccc
     4261 gatccgctcg tacagaagat cagccgcgaa cgcttcatgg ccgactggtc acacttcctc
     4321 gcgcacttct ccgagatcga agacgcgcgc ttcaaagtta aagaagccta cttcgacgca
     4381 gacgcgcagg ccgtggcggg cgcaaaagag ccgacggcgg tggtcggcgc gaagggcaag
     4441 gcgcgcgtcg ccttctacgt catcgggcgc gaccttgacg gcagacgcga atgggcgcgc
     4501 ggcgtcgcaa acgtcaacgc gcgctacgac gcttccaatc gttggcagtt cgattcgttc
     4561 ctgctcgtct cgctcgattc gctcgtcgcg acaagcgatc tcttctccga ggtggcgatt
     4621 ccggcagggc tcggcgcgag cctcccggcc ttcggctcgc ccggcaacgg cggcttcgtc
     4681 tggcacggcg cggcggcctc cgacttcaac caggacggcg cgattgatct cttcgtcacc
     4741 gcgcccgcgc gcaacttcct ctatctcaac gacggcaaag gacgcttcac ggacgcatcc
     4801 gacgcggcgg gcgtgcgcct gcttgcggcg ggcgtcgcgc cgctcgtctt cgactacgac
     4861 aacgacggcg acggcgacgt gttcgtctcg aacgtcggcg cgcaagtcct gttcgagaat
     4921 cgcctcgtgc ccgacggcaa gttggagttt cgagacgtgt cgcaggaggc gggcgtcggc
     4981 ggcgtgcagg ccatcggctt ctccgcggtc gcaggcgacg tgaacaacga cgggcgacag
     5041 gacatctacg tcacttcata taatcgctac ggccagatca cgcccgactc ctggtttcgc
     5101 gcgacgaacg gcacgccgaa tctcctcttc atcaatcagg gcaacggcac gttcaaggaa
     5161 gaggcgaagc gttggggcgt ggacgacagc cgttggagct acgcggcggc cttcgccgat
     5221 gtgaacgcgg acggcagagt cgatctctac gtcgccaacg atttcggcga gaaggccatg
     5281 ttcatcaatc gcggcgacag gttcacggac gaagcgaaag agcgcggcgt gctcgatccc
     5341 ggcaacggca tgggcgtctc cttcggcgat tacaacaacg acggcctctt agacgtgcac
     5401 gcctcgaaca tgtcttcgac ggcgggcaac cgcatcctcg cgcgtctctt cccgaaccag
     5461 agtccgaagg agaacgtctt gaagaaactc gcggcgggca acaacctctt cgagaatcag
     5521 ggcgacggaa agttcaaaga cgtgactgcg gaggtcggcg gcttcggtgg cggctgggct
     5581 tggggcggcg gcttcataga tttcgacaac gatggctggg aggacatcta cacgccgaac
     5641 ggctttattt ccggcaaatc catgaaggac acttgaagcc acttctggcg tcaggtcgtg
     5701 acgcaaaaaa atgatggcaa ggatgagaag ggcgcgcttc gagttgacca gacgcagttg
     5761 atgttcggcg agggtttttc gttcagcggc tacgagcgcg acccgctcta tctgaatctc
     5821 ggcacgaaga agttcatgga catctccggt gtctccggca tcgattcgat cacggacggg
     5881 cgcgcgggcg tcttcgcaga cttcgacaac gacggcgacc tcgacgtgtt ctcgacgacg
     5941 attcagggtc aagcccatct cctgtttcgc aacaacgtcg ggcaggacgg aaaccacctg
     6001 cgcatcgcgc tcgaaggcaa cgaacggacg ggacgcgacg ctttcagcgc ggtcgtccgc
     6061 gtcaagactt cgcagggcac gctgacgaag attaaagacg gcggtgcggg cttcatctcg
     6121 cagcacgacc cgcgcctgct cttcggctta gggcgcgacg agcgcgccga atggatcgaa
     6181 gtgacgtggc cgcacggcaa ggtcgaacgc ttcgcgggcg aggcgcgcgc cggaacgtcc
     6241 ctcctgcttc gtgaaggcac ggggcgcgca caagagttaa cgctcgcccg cgcgaagttg
     6301 cccgatccgc tgacgcgcgc ggaggcgttt gcgcgcggct tgaagatcaa gatcgggcag
     6361 ccgctcccca cgctcgcctt gaagtcgctg gatagcgtcg ccgctgctgg gcgcagtgcg
     6421 tcgcgccccg gagcctattt gcgccccggt cgccgcacgc tcatcaacgt ctgggcgacg
     6481 tggtgcacgc cctgcgcggc ggagatgccg gaactcgaaa aactgcgcgc aggcttgaac
     6541 acgcgcggca ttgatctcgt ggggttgaac gttgacagcg agccgaaggc ggatgtgcgc
     6601 ggcttcctca gagagcatcg cgtgggttat ccgaacttcc tcggcggcgt cgcggccatc
     6661 gagagcctgt atgcgacgga tgaactgtca gtgccgatgt caatcgtcgt tgacggacgc
     6721 ggcattgtga cggagatcat tccgggctgg tctgcggaga cgcagagacg tttccgcgaa
     6781 ctcgcgggcg aaggcgcggg cagcggcggc agtagcggcg cgcccgccgc gaagacgaag
     6841 gcgcggagtc taccgcgtcg ctgaaggcgt cggcgatcct gcgcggtctt tcaaaaattc
     6901 gctgatggct ttctctgcgc ctccgtcgtg agtctgattt ctcgcgggag gcgcaaagaa
     6961 aattaaaggt agttttcaaa cgcactcgtc gtcctgaaac ttggaggagg aagcatgaac
     7021 agaaacgtaa gtggtcgcct ggcagttcta tttgcggtct taactttcgc gctcatcccg
     7081 gcgggcgtta acgcgcaagt catcattacg ggcgcgcaac tctcgcgcat cgtaccggcg
     7141 ggtttctact tcgaagggca gagcgcgccc acgcagatgc gcaacgcatc ggcggcacgc
     7201 ttcggcgcga agcagcacct cgtcgtcggc atggttgaca cttccggcta ttcggctgac
     7261 gtgcgttcga gatacgaagg cttcttcatc atcgacacgc cgatgatggt cggcgggcag
     7321 gaactcggca tcggcgcgta cggcttcggc ttcgtggaga acaacaggtt caccatctcg
     7381 gacgtggccg gtaatcaaat cttcaccgtc aacacggagc ctgaccggaa cctgcgccgc
     7441 ccgcgaccgc tcgtgttgac gcgcggcgcg ggcggcgtgc gcctctacag cggtcgtcgt
     7501 tatgtcgcca tcaccttgcg gtaaagactt cctcttctgt tgatgcaatg gatgcgtgcg
     7561 tgagtttttt caacgcggag agcacagaga tgaagacgca gaggacgcag aggacttcaa
     7621 gttcctctg
//
LOCUS       DASWNS010000082         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_28980, whole genome shotgun sequence.
ACCESSION   DASWNS010000082 DASWNS010000000
VERSION     DASWNS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28980"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VGB76_02820"
     CDS             complement(<1..834)
                     /locus_tag="VGB76_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY0097864.1"
                     /translation="MKAIIVEEYGGADKLLYREVEQPAPKAGEAVVRVEAAGVNYIDI
                     YHRTGLYPLPLPFTPGSEAAGTVAAVGEGVTEIEVGDRVAYAMALGSYAELTTVPAAR
                     LVKVPEAIDTQTAAAAMLQGMTAHYLVTSTYPLKRGETALVHAAAGGVGLLLIQLAKR
                     IGARVFGTVSTKEKAALAREAGADEVFIYTEQDFQEETRRANGGAGVEVVYDSVGRTT
                     FRQSLDSLAPRGMLALFGQSSGTVPPFDAALLAQKGSLFLTRPSLGHYTRTREELLWR
                     AG"
     gene            complement(938..1963)
                     /locus_tag="VGB76_02825"
     CDS             complement(938..1963)
                     /locus_tag="VGB76_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097865.1"
                     /translation="MLNPARTPFGAIFYNVDTLYHDPESMHTLRPPSFEVQLEQPERR
                     EKVSLPEVATANEGGRANFAKLRAALGVEFRLLRAERSLVVIMPLAIFLSVLELAFYE
                     VPPDISYTAAYASSTAKSLLLFLYGLTVFYTGEAIQRDREVRIEPLLWSAPAANYVSL
                     VSKFLAPVVLGLSLLALSVLAAIALQIYKGHTPVEFSAYLLVYSLILIPSLFFIAAAS
                     VLLNVSLRDKYLAYAVSIAIGGGLFYLYKQGYNHWSYNPVLYQLWTYADLTGAGSNGA
                     RILTHRIYFLAIACASLALAHLFHQRRATKGFRVNARLGSASWSILVAAVSVVVAVVA
                     GFVLVRL"
     gene            complement(1965..2849)
                     /locus_tag="VGB76_02830"
     CDS             complement(1965..2849)
                     /locus_tag="VGB76_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0097866.1"
                     /translation="MLSIDSLRKTYASRVQALKGVSLEVPAGMFGLLGPNGAGKTTLM
                     KILATLLKPDAGAAQMNGLDLISHPDGARRILGYLPQEFGLYPTLTAEQTLDYFARLK
                     GVNEASERRTLIETLLERVNLSAARKQRVGEFSGGMRQRLGIAQALIGQPTLIIVDEP
                     TAGLDPEERVRFHNLLSDTAGEGRVVILSTHIVSDVSNLCSRMAIIRHGEILASGTPQ
                     EAIGELAGSIWEASVPRENVAALKSRFRVISSQMFDGRARLKVISKGVRPSEEFVAAT
                     PTLEDYYFHLVSQPERAA"
     gene            complement(3050..3448)
                     /locus_tag="VGB76_02835"
     CDS             complement(3050..3448)
                     /locus_tag="VGB76_02835"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEY0097867.1"
                     /translation="MKRANGTFEVKLNPQADVEVGDASVARLSLEKQFRGDLEAVSRG
                     QMLAVHAEVKGSAGYVAMERVSGTLEGRSGTFALQHSGTMTRGEGQLTITVVPDSGTG
                     QLAGLSGKMSINIADGQHSYDFEYTLAQPD"
     gene            complement(3503..>4149)
                     /locus_tag="VGB76_02840"
     CDS             complement(3503..>4149)
                     /locus_tag="VGB76_02840"
                     /inference="COORDINATES: protein motif:HMM:NF025638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEY0097868.1"
                     /translation="LMEKLLARRQDADTECDKCGNRFTLWDALEKKFASAEVRRQVEE
                     LQANDVIRLDSRRKGKLLALEVGARITSADQKCFEIPATEDEGLDMELEFTDDNGKGT
                     GRRLYLQLKAGNSYLRKRKDGAEIFTIKKQGWVNNWLAQPHPVMLVIGTFAGEDERIP
                     GKDRLEFAEVRWMEISSYLKRASDNGRKPVRQIVFAGERLDATSVRRWRDRVLH"
BASE COUNT          791 a         1298 c         1212 g          848 t
ORIGIN      
        1 tcccgcgcgc cacaacaact cttcgcgcgt gcgcgtgtag tggccgaggc ttggccgcgt
       61 taaaaagagc gatcccttct gcgcgagcag ggcggcgtca aagggcggga cagtgccgct
      121 cgattgaccg aacagggcga gcattccgcg cggcgcgagc gagtcgagac tctgcctgaa
      181 ggtcgtccgc ccgaccgagt cgtagacgac ttcgacgcca gcacctccgt ttgcgcgccg
      241 tgtctcttcc tgaaaatcct gttccgtgta gatgaagact tcatccgcgc ccgcctcgcg
      301 cgccagcgcg gccttctctt tcgtggacac agtgccgaac acgcgcgcgc caattcgctt
      361 tgccaactga atcagcagca gcccgacgcc gcccgccgca gcgtgtacga gagccgtctc
      421 tccgcgcttg agcgggtaag tcgaagtgac cagatagtga gccgtcatgc cttgcagcat
      481 ggccgccgcc gccgtctgcg tgtctatcgc ttcgggcact ttcacgagtc gcgcggcggg
      541 caccgttgta agctccgcat acgagcctaa agccatcgcg taggcaaccc tgtctccgac
      601 ctctatttcg gtcacgccct caccgacggc tgccactgtg cccgccgctt ccgaccccgg
      661 cgtgaaggga agcggcagcg ggtagagtcc cgtgcgatga tagatgtcta tgtagttaac
      721 accggcggct tccacgcgaa cgaccgcctc gcctgccttc ggcgcgggct gttccacctc
      781 cctgtagaga agcttgtctg ccccgccata ctcttcaacg atgatcgctt tcatcccggt
      841 tccttcgcgt cgtgctgcgc cagctacgag tctcccttcc gccgggagcg aaggccggag
      901 cgaatccggc ctcgatagta aaagtgtctt gagtatatta aagccgaacg aggacgaagc
      961 ctgcaacgac ggcgacgacg accgagacgg cggcgaccag aattgaccaa ctcgcgctgc
     1021 cgagacgagc gttaactcga aatcccttcg tcgctctgcg ctggtgaaag aggtgcgcca
     1081 gcgcgagaga cgcacaggcg atggcgagaa agtagattct gtgcgtgagt atgcgcgcgc
     1141 cgttgcttcc cgcgcccgtc aagtccgcgt aagtccagag ttgatacaac accggattat
     1201 aagaccagtg attgtagccc tgtttgtaca ggtagaacag cccgccgccg atcgcgatgc
     1261 tcacggcata agcgaggtac ttatcccgca gcgagacgtt cagcaacacg gaggcagcgg
     1321 cgataaaaaa caggctcgga atcaggatca acgagtagac cagaaggtag gccgagaact
     1381 cgaccggcgt gtggcctttg tagatttgca atgcgattgc cgccagcacg ctgagcgcga
     1441 gcagcgacag gccgagcacg accggggcga gaaacttcga gacgagcgag acatagtttg
     1501 cggcgggcgc gctccacaag agcggctcga ttctcacttc acgatcacgt tggatggctt
     1561 cgcccgtgta aaagaccgtg aggccgtaga gaaagagcaa gagcgacttc gcggtgctgc
     1621 tggcgtaagc tgccgtataa gagatgtcgg gcggaacttc atagaaagcg agttccagca
     1681 cggagaggaa gatcgccaga ggcatgatca cgacgagact tcgttcggcg cgaagcaggc
     1741 gaaactcgac gcccagagcc gcgcgcagtt tggcgaagtt cgcgcgccct ccttcgttcg
     1801 ccgtggcgac ttcgggaagc gacactttct ctctgcgttc cggctgttcg agttgcactt
     1861 cgaacgaagg cgggcgcaac gtgtgcatgc tctccgggtc gtgataaagc gtgtcaacgt
     1921 tgtaaaagat tgcgccaaag ggcgtgcgtg cggggttcaa catctcacgc ggctctctcc
     1981 ggttggctga cgagatgaaa atagtagtct tcgagcgtcg gggtcgccgc gacaaactct
     2041 tcgctcggtc gcacgccctt cgagatcact ttgagacgcg cccgcccgtc gaacatctgc
     2101 gaagagataa ctctgaaacg cgacttcaac gccgccacgt tttcgcgcgg cacgctggct
     2161 tcccagatcg agcctgcgag ttcgccgata gcttcctgcg gcgtgccgga cgcgagtatt
     2221 tcgccgtgcc gtatgatcgc catgcggctg cacaggttcg acacgtcgga gacgatatgc
     2281 gtggagagga tgaccaccct gccttcgccc gccgtgtcgg agagcaggtt atgaaaacgc
     2341 acgcgctcct ccgggtcgag tcctgccgtc ggttcatcga cgatgatgag cgtgggttga
     2401 ccgatgagag cctgcgcgat gccgagacgc tggcgcatcc cgcccgaaaa ttccccgacg
     2461 cgctgctttc gcgccgcaga taaattgact ctttcgagca gcgtctcgat caaagtcctg
     2521 cgctccgacg cttcgttgac gcccttgagc ctggcgaagt agtcgagcgt ctgctccgcc
     2581 gtgagagtcg ggtacagacc gaattcctgc ggcaggtagc cgagtattct gcgtgctccg
     2641 tcagggtgcg agatcaggtc gaggccgttc atctgcgccg cgcccgcatc gggtttgagc
     2701 agcgtcgcca gtatcttcat cagcgtcgtc ttgcccgcgc cgttcggacc gaggagtccg
     2761 aacattcccg caggcacttc gaggctcacg cctttcaaag cctgcacgcg gctggcgtag
     2821 gttttcctga gactgtctat gctaagcatc gtcgatacta caggggcacg caggcagcgg
     2881 agatcaattc acgccggttt ctaaatcgta gagcttaacg atttgaaacc atagacacct
     2941 gtagcgcgaa atttgttccg gcacggcgca atcgctcgac gcgaagccgg ggcgcgcggc
     3001 tgcatagcca tcctgtgaag gccgccgccg ggggcaggtg gccgaaagct taatcgggtt
     3061 gcgcgagcgt gtactcgaag tcgtaagagt gctgcccgtc tgcgatgttg atcgacatct
     3121 tgcctgacag tcctgcaagt tgacccgtgc cggaatcggg cacgaccgtg atcgtgagtt
     3181 gcccctcgcc gcgcgtcatt gtgccactgt gttggagcgc aaaagtgccg ctgcgccctt
     3241 cgagcgtccc actcacgcgc tccatcgcca cgtatccggc ggagcctttc acctccgcgt
     3301 ggaccgcaag catctgccca cgactcacgg cttccagatc gccccggaac tgcttctcaa
     3361 gcgacaacct cgccacactc gcgtcgccca cttcaacatc cgcctgagga ttcaacttca
     3421 cttcaaacgt tccattcgca cgcttcatca agacaggctc cttaattgat ccaagttgct
     3481 aggttttgaa tacgagtaaa ttttagtgca acactctgtc ccgccagcgg cgcacgctcg
     3541 tggcgtccag acgctcgccc gcaaagacga tctgcctgac gggcttcctg ccgttgtcgc
     3601 tggcgcgctt caggtaactg ctgatctcca tccaacgcac ctctgcaaac tccagtctgt
     3661 ctttgccggg gatgcgctcg tcctctccgg caaacgtgcc gatcaccagc atcaccggat
     3721 gtggctgcgc gagccagttg ttcacccatc cctgtttctt gatcgtgaat atctctgcgc
     3781 cgtcctttcg cttgcggaga taggagttgc ccgctttcag ttgcaggtag agtcgccgcc
     3841 cggtgccttt gccgttatcg tctgtaaact ccagttccat gtcgagtcct tcatcttcgg
     3901 tggccggaat ttcgaagcac ttttggtctg cgctggtaat gcgtgcgccg acctctagcg
     3961 cgagcagttt gcctttacgg cgcgagtcca gccggatcac gtcgttggcc tgaagttcct
     4021 ctacctgcct gcgcacttcc gcactcgcga actttttctc cagcgcgtcc cagagcgtga
     4081 aacggttccc gcacttgtcg cattccgtgt cggcgtcctg tcttctggcg agcagctttt
     4141 ccatcaaca
//
LOCUS       DASWNS010000083         5805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_471224, whole genome shotgun sequence.
ACCESSION   DASWNS010000083 DASWNS010000000
VERSION     DASWNS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471224"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VGB76_02845"
     CDS             <1..1080
                     /locus_tag="VGB76_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0097869.1"
                     /translation="VQTEIISYLRDQRQREEAKRFADKLRAASNVKILLPNATPPRND
                     AERARVLAVVNGEQLTSGHIEDSLKPLIYNVQERAYLLRKNQLDVRINDALLEQEAQK
                     RKITTKALLEAEIDAKAQPVTEAEARAFYDQNKERINGDFAQVKDQIVQYLQETQKRN
                     LQTAFADQLRKAATLQINLRAPEAPVLQISTDDQPSKGSPTAPVTLIEFTDFQCPSCG
                     MAQPILERLTTEYGDRLRLVVRDFPLEQHEFARKAAEAAEAARAQGKYWEYASLLFTN
                     QKELGVAKLKEYATQVGLDRAKFDAALDSGQFADKVQRDFQEGMLLGIGSTPTFYVNG
                     RITNDRTYEGLKAAIEKALAEKTKK"
     gene            1092..1601
                     /locus_tag="VGB76_02850"
     CDS             1092..1601
                     /locus_tag="VGB76_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097870.1"
                     /translation="MKQQRKKGREVILLLAPSRVAEPPATIQNQTSNEVDIELRERQF
                     FIEQKLSSLHTAGIVCITWWVTLVVFCGTILAATWLHKEKLVQTTPMKWTLFAVLTIF
                     FLGAVGFGVLVIAYLRKFKGDVARFPENSNEGRLFNTELGFFQGGMWIGTVSFILILM
                     AWVVFWLAL"
     gene            complement(1998..3278)
                     /locus_tag="VGB76_02855"
     CDS             complement(1998..3278)
                     /locus_tag="VGB76_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEY0097871.1"
                     /translation="MTERVQRRATQSGDAFPPVEAIRAHFPALERSYGGHAVAYFDGP
                     GGTQVPRAVVEAIADYLYGHNANTHWAYPASAETDALIEEARHALADFLNAAPNEIAF
                     GANMTTLTFHLSRALGRQLNAGDEIVVTELDHHANVAPWQALADERGLRVRMAKMLPE
                     TGQLDWDDFSRLVNGRTRLVAIGAASNALGTISDVRRAVDLAHEVGALCFVDAVHFAP
                     HQLVDVRALDCDFLACSAYKFCGPHLGVLYGKQELFESLDFPKLLPAPDTAPERVETG
                     TQNHEGITGTAAAINFFASLAPAAAAANESRRERLRTTFAALHARGSDLTAQLWQGLF
                     DIKGVRLYGPLPGEPRTPTIAFTVKGVASTSVAQKLAALGLFLSHGDFYAATVVGRLG
                     LGNEGLVRAGCACYTTQDEIERLIRGVREIARRT"
     gene            complement(3315..3566)
                     /locus_tag="VGB76_02860"
     CDS             complement(3315..3566)
                     /locus_tag="VGB76_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097872.1"
                     /translation="MRVEQIEELARQALLESRRNDCEIVSVKAKDGAENSWSIDLLDV
                     LHTQEPFRVTLEVAPASSEEEIKEAIRRGIAEHFSAASY"
     gene            complement(3645..4913)
                     /locus_tag="VGB76_02865"
     CDS             complement(3645..4913)
                     /locus_tag="VGB76_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0097873.1"
                     /translation="MKRVSLRAAFGVALMLACLSVEVAAQGAVAGAAAGGNWPQWRGP
                     NRDGISRETGLLKQWPAEGPPLVWKTTGAGRGYSSLAVSDNRLYTMGLRGDREFIVAF
                     DVKSGKELWAMPHGKAFHNDRGDGPRGTPTIEGERLYALGGNGDLSCLETRTGRIVWT
                     MNVLQKFGGSNITWGISESPLIVGDKLLVNAGGQNASIVALNKRDGALIWKSQSDEAG
                     YSSAITMDAGGTPQVVFFTSRRAVGLDLRDGKLLWEYSRAANRTANAATPVARGNRVF
                     ISSDYGTGAGLIEIKAGERGALTAQEVYFTKDMRNHHSSSILVGDHLYGFSSGILTAM
                     RFDSGEVAWKDRSVGKGSIVYADGHLYCLSENGVVGLVEATPEAYREKGRFRIAQDSL
                     PTWTHPIIAGGRLYLRDQDTIYAYDVRQKK"
     gene            5228..>5805
                     /locus_tag="VGB76_02870"
     CDS             5228..>5805
                     /locus_tag="VGB76_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097874.1"
                     /translation="MRKLICLCLLLLALPPNSLAQEETYEQLVKRYDYDRSTPLDLRD
                     FGVVRREEGVRVHDISYASLKGGRVPAYLVVPEGKGPFAAILFGHWAMKGSPTRNRTE
                     FLDEAVALARAGAISLLPDAPFARHGFKETTQPFDPRDIETYFQQVLDMRRGIDLLVA
                     REDVDPARLAYVGHSYNANVGGVLAGVEKRIKT"
BASE COUNT         1179 a         1750 c         1757 g         1119 t
ORIGIN      
        1 gttcaaaccg agatcatctc ttacctgcgc gatcagcgtc agcgcgaaga agcgaaaaga
       61 tttgccgaca aactgcgcgc cgcctcgaac gtcaagattc ttctccccaa cgccacgccg
      121 ccgcgaaacg acgccgaacg cgcacgcgtc ctcgccgtcg tcaatggcga gcaactcact
      181 tcgggtcaca tcgaagattc gctcaagccg ctcatctaca acgtgcagga gcgcgcttac
      241 ctgctgcgca agaaccaact cgacgttcgc atcaacgacg cgctgttgga gcaggaggcg
      301 caaaagagaa agatcacgac gaaggctctg ctcgaagcgg agatcgacgc caaggcgcag
      361 cccgttacgg aagccgaggc gcgcgccttt tatgatcaaa acaaggagcg catcaacggc
      421 gactttgcac aggtgaaaga tcagatcgtg caatacttgc aggagacgca gaaacgaaat
      481 ctgcaaacgg cttttgccga ccagttacgc aaagcagcga cgctccagat caatctgcgc
      541 gcgccggaag cgcccgtgct gcaaatctcg acggacgatc agccgtccaa aggcagcccg
      601 accgcgcccg tcacgcttat cgagttcacg gacttccagt gtccgagttg cgggatggcg
      661 cagccgattc tggagcgact gacgacggaa tacggcgacc gcctgcgtct ggtcgtgcgc
      721 gatttccctc tggagcagca tgaatttgcg cgcaaggcgg cggaagcggc ggaggccgcg
      781 cgcgcacagg gcaagtactg ggaatatgcc tcgctgttgt tcaccaacca gaaggaactc
      841 ggcgtggcta agttgaaaga gtatgccacg caggtcggtt tagaccgcgc gaagttcgac
      901 gccgcgctcg actccgggca gttcgcagat aaagtccagc gcgactttca agagggcatg
      961 ctcctcggca tcggcagcac cccgaccttc tacgtcaacg ggcgcatcac caacgaccgc
     1021 acctacgaag gactcaaagc cgccatcgaa aaagctctgg cggagaagac taaaaagtga
     1081 agagcattaa ggtgaagcaa caacgaaaga aggggcgaga agtgatactg cttctcgccc
     1141 cttcacgtgt tgccgaaccc ccggcgacaa ttcagaatca gacgagcaac gaagtagaca
     1201 tcgaactcag agagagacag ttctttatcg agcagaaact ttcgtcgctg cacacggctg
     1261 gaatcgtctg catcacgtgg tgggttacgt tggtggtttt ttgcggcacg attctggccg
     1321 cgacgtggct gcacaaggag aaacttgtac agacgactcc catgaagtgg acgctctttg
     1381 ccgtgctgac gatcttcttc ctcggggctg tgggcttcgg ggttctggtc atcgcgtacc
     1441 tcaggaagtt taaaggggac gttgcccgtt ttcccgaaaa ctcgaatgaa ggtcgtctgt
     1501 tcaacacgga gttgggattt tttcaggggg gaatgtggat cgggacggtc agtttcatct
     1561 tgatcctgat ggcctgggtc gtcttctggc tcgccctgta gtgtttcgga tactaacgcc
     1621 cctcacccgc ctcgcagcac aggcgcgggt tcgctccagc cctcccgacc gtcttcacag
     1681 ctatccaact tttgagtgaa atttagcgcg acgtagctct gcggctagcg actacagaag
     1741 cgaacacccg tcggagatgt cctcacagca tccgcgacgg gcttgttcaa tttccctgtg
     1801 cgccgggcgg cgcaggatgg gaagttgttt gttcgccttt tgaccgttga cggtcgcggc
     1861 gtagggtgtc ctcactacac cagacgcctt ttgttgcgcg gaggctatca ccgttccccg
     1921 tacgcgtcaa taacaattaa cagcaaaaat tcgggcgtcg gacgagtctg aaatctacgc
     1981 gcgacggcct cggaaaacta cgtgcggcgg gctatctcgc ggacgccccg gatgagcctc
     2041 tcgatctcat cctgcgtcgt atagcaggcg caaccggcgc gcactaaacc ttcattgccg
     2101 aggccgaggc gtccgacgac agtggcggcg tagaagtcgc cgtgggagag gaagagtccg
     2161 agtgcggcca gtttttgcgc gaccgaagtg gaggcgacgc ccttgacggt gaaagcgatg
     2221 gtcggcgtgc gcggctcccc cggcagtgga ccataaagcc ggacgccttt aatgtcaaac
     2281 aagccttgcc agagttgcgc cgtcagatcc gaaccgcgcg catggagcgc ggcaaacgtc
     2341 gtgcgcagac gctcgcggcg tgattcgttc gctgcggcag cggcgggtgc gagcgaagcg
     2401 aagaagttga tggcggcggc agttccggtg atgccttcgt ggttctgcgt gcctgtctcc
     2461 acgcgttcgg gggcggtgtc gggagcgggc aacagcttgg gaaaatcgag cgactcaaac
     2521 aactcctgct tgccatagag cacgccgagg tgcgggccgc aaaacttgta ggcggagcaa
     2581 gcgagaaagt cgcaatcgag cgcgcgcacg tccacgagtt gatgcggcgc aaagtgtacc
     2641 gcatccacga agcacaaagc tccgacctcg tgcgccaagt ccaccgcccg gcgcacgtcc
     2701 gagatcgtgc cgagcgcatt tgaagccgcg ccgatggcga ccaacctggt gcgcccgttg
     2761 acgaggcggg aaaaatcatc ccaatcgagt tgtcccgtct ccggcaacat cttcgccatg
     2821 cgaacacgca ggccgcgctc atcggccaac gcctgccaag gggcgacgtt cgcatgatga
     2881 tcgagttcgg tgacgacgat ctcgtcgccc gcattaagct gccgaccgag cgcacgcgag
     2941 agatgaaagg tgagcgtcgt catgttggcg ccgaaagcga tctcgttggg ggcggcgttg
     3001 aggaaatctg cgagcgcatg ccgcgcctct tcgatcaggg catcggtttc cgcgctggcc
     3061 ggatacgccc agtgtgtgtt ggcattatgc ccgtagagat aatcggcgat agcttcgacg
     3121 acggcgcgcg gaacctgtgt gccgccgggt ccgtcgaagt aggcgaccgc gtggccgccg
     3181 taggaacgct caagggcagg gaagtgcgct ctgatcgctt cgaccggcgg gaaggcatcg
     3241 ccgctctgcg tcgcgcgccg ctgtactctc tctgtcatgt gggttttgtc gaaaagttat
     3301 ggcggctgtc aggatcaata gctggccgcg ctgaaatgtt cggcgatgcc gcgccggatg
     3361 gcttctttga tctcctcttc gctgctcgcg ggcgcaactt ccagcgtcac gcgaaacggt
     3421 tcctgtgtgt gcagcacatc gagcagatca attgaccacg aattctccgc cccgtccttc
     3481 gccttgacgg acacgatctc gcaatcgttc cgccgcgact ccaacaacgc ctgccttgcc
     3541 agttcttcta tttgctccac acgcatctgt cgtctctccg cttgagttaa tcgaacgttc
     3601 caaccttcgc cgcctgtcaa cggctgataa ggaatgataa gaggttactt cttctgccgc
     3661 acgtcgtagg cgtagatggt gtcctgatcg cgtagataga gacggccacc ggcgatgatc
     3721 gggtgagtcc aggtcgggag cgagtcctgc gcgatacgga agcgtccctt ctcacggtag
     3781 gcttcgggag tggcctcgac gaggccaacc acgccgttct cgctcaagca gtacaggtgg
     3841 ccgtcggcgt agacgatgga gcctttgccg acgctgcgat ccttccatgc cacctctccg
     3901 ctgtcgaaac gcatggccgt caaaatgccg ctcgaaaaac cgtagagatg atcgccgacg
     3961 agaatcgaac tgctgtgatg attgcgcatg tccttcgtga aatagacttc ctgcgccgtc
     4021 agcgcgccgc gctcgcccgc cttgatctcg atgagtcccg cgcccgtccc gtaatccgaa
     4081 gagatgaaga cgcgattgcc gcgcgccacg ggcgtcgccg cgttggccgt ccggtttgcc
     4141 gcgcgcgagt attcccacag gagtttaccg tcgcgcaggt ctagcccgac ggcgcggcgc
     4201 gaggtgaaga agacgacctg cggcgtgccg cccgcatcca tcgtgatggc cgaggaataa
     4261 cccgcctcgt cgctctggct cttccagatg agcgcgccgt cgcgtttgtt gagtgcgacg
     4321 atggaagcat tctgcccgcc cgcgttgacg agcagtttat cgccgacgat gagcggcgat
     4381 tcgctgatgc cccaggtgat gttcgacccg ccaaactttt gcaggacgtt catcgtccag
     4441 acgatccgcc cggtgcgcgt ctccagacac gagagatcgc cattgccgcc gagcgcatag
     4501 agacgttcgc cttcgatggt gggcgtgccg cgaggtccat cgccacggtc gttgtggaag
     4561 gctttgccgt gcggcatcgc ccagagttct ttgccgctct tcacgtcgaa ggcgacgatg
     4621 aattcgcggt cgccgcgcaa gcccatcgtg tagaggcgat tgtccgagac ggcgagcgac
     4681 gaataaccgc gtcctgcgcc cgtcgtcttc cagacgagcg gcgggccttc ggcaggccat
     4741 tgcttgagca agcccgtctc tctggagatg ccgtcgcggt tcggaccacg ccactgcggc
     4801 cagttaccgc ctgctgctgc tccggcgact gcgccctgcg ccgccacttc aaccgataag
     4861 cacgccagca ttaacgcgac accgaacgca gccctgaggc tcacacgctt cataaagaaa
     4921 actcctcctg caaacattgc ctgcttcgaa ttccgtcagc gagttgcccg attatagcaa
     4981 caccgcgcgc cgcacacgct aaaaaacatc gccgtgcggg atacatattt ttggaacgaa
     5041 cggccaacgg cggctcaaga agggagccgt catagtgtat cttgaagttg aaagatttac
     5101 tgccgggaag agtttgcagc gcggcggctg agcatgacat caaacgcgcg cacaagttgc
     5161 cttagccgtc ttcctgcccg gcgtccgttg cctgagtctg aaatatccga cacttgagga
     5221 gcgtcttatg cggaagttga tctgcctctg tttactgttg ctcgccctgc ctccgaactc
     5281 tctcgcgcag gaagagacgt atgaacaact ggtgaagcgt tacgattacg accggagcac
     5341 gccgctcgac ttgagagact ttggcgtcgt gcggcgcgaa gagggcgtgc gagtccacga
     5401 catctcttat gcgagtttga agggcgggcg cgtgcccgcg tatctcgtcg tgccggaagg
     5461 caaaggaccc ttcgccgcca tcctcttcgg ccattgggcg atgaagggat cgccgacgag
     5521 aaatcgcacg gagtttttgg acgaggccgt tgcgctggct cgcgccggtg cgatctcgct
     5581 gctgcccgac gcgcccttcg cgcgtcacgg cttcaaggag acgacgcagc cgtttgaccc
     5641 gcgagacatc gaaacctatt ttcaacaggt gttggacatg cgacgcggca tagacctcct
     5701 cgtcgcacgc gaggacgttg accccgcgcg gttggcctac gtcggccaca gctacaatgc
     5761 caacgtcggc ggcgtgctcg cgggtgtgga gaagcgcatc aagac
//
LOCUS       DASWNS010000084         6025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_471267, whole genome shotgun sequence.
ACCESSION   DASWNS010000084 DASWNS010000000
VERSION     DASWNS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471267"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VGB76_02875"
     CDS             complement(<1..334)
                     /locus_tag="VGB76_02875"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0097875.1"
                     /translation="MLPIELQTPLDIRRALARRVKALRLERGWTQQEVAERAGIALPT
                     YRVFERSGRISLDRLLKLAVIFEARAGFDQLFAPRPVRSLAELERRAERPSRKRGRRG
                     DAQTRSTAG"
     gene            556..771
                     /locus_tag="VGB76_02880"
     CDS             556..771
                     /locus_tag="VGB76_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097876.1"
                     /translation="MSRAEERLLNPPPGGSIEAARAHGVDLSLLVERLRKTPEERVRD
                     LQRAVKVIEAARGKALKASRVRGGAID"
     gene            1158..1436
                     /locus_tag="VGB76_02885"
     CDS             1158..1436
                     /locus_tag="VGB76_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY0097877.1"
                     /translation="MIKQVTITDLQRRAAEVISSLREGPVVVNQRGRPVAVILTAEAY
                     EQMEQAVAEVEAKQVREIVEAGLSSYGAGRTSPQAAVAQRVRACRRKK"
     gene            complement(1614..2228)
                     /locus_tag="VGB76_02890"
     CDS             complement(1614..2228)
                     /locus_tag="VGB76_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock and DUF1294 domain-containing
                     protein"
                     /protein_id="HEY0097878.1"
                     /translation="MRHQGRIAKWNDERGFGFISPSEGGGSVFVHISSFPRSDRRPGV
                     NEAVSYTLAFDSHGRPQANDVRFVVGTRSASPTRGIPSAPITFAISFLAAVTALVVSG
                     RLEMSWLLLYYGASIITYGVYARDKTAAQNAGRRVPESTLHLMSLVGGWPGALIAQVL
                     LRHKTRKTSFLIGYWLTVIVNCVALGVVLVKGVSPVKVLLGTAT"
     gene            complement(2389..2976)
                     /locus_tag="VGB76_02895"
     CDS             complement(2389..2976)
                     /locus_tag="VGB76_02895"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEY0097879.1"
                     /translation="MPAIALRRTICWLPTWNKGREGVGLEHLLLDEHVADSVVLAFDE
                     GRGPFRLTYRLTWDELWRLREADLVVTKERFTTSLNLRTDGRGRWRDDDGRAIDELDG
                     CVDIDIWPTPFTNSFPVRREPMVVGERRQFRMAWVFAPDLTVRPQPQAYTRLADRLYL
                     FESLDGSGFSVELPVDEDGIVLDYPGMFRRLVDSE"
     gene            complement(3071..3391)
                     /locus_tag="VGB76_02900"
     CDS             complement(3071..3391)
                     /locus_tag="VGB76_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097880.1"
                     /translation="MRLLFDQGTPVPLRNHLPYHTVETAYERGWHDLKNGELLARAEA
                     EGFDALVTTDQNIRYQQNLAGRRIGVLVLMTTSWPRIRGDVTRVAQAIDNLHAGGYEE
                     IAFP"
     gene            complement(3394..3624)
                     /locus_tag="VGB76_02905"
     CDS             complement(3394..3624)
                     /locus_tag="VGB76_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY0097881.1"
                     /translation="MMDWSTCTAVEQDPEKVSGSWVFRGTRVPVVALFENLEGGASVE
                     DFLSWFPGVTREQVEAVLECAIESLRPGRQAA"
     gene            4422..5408
                     /locus_tag="VGB76_02910"
     CDS             4422..5408
                     /locus_tag="VGB76_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0097882.1"
                     /translation="MKKRTIGIVVIFIILIIAVIAYQIYSPHNVTQESTVIRIGYKPN
                     SGYQHFFIAQEKGYFKKYGLNVEGVTFESTNQMVQAVVNGDLDATAASSIEVIGLVEQ
                     NSPNLLKIYLTLVFDKDHAFHSVLVPPDSKIQTLADLKGKKIGTVPGSTSQSWLEIIL
                     GRFFDSKKDVQIIQLEPRLQLQALSSGQVDALYTVDPIVAQAQAKGLARVLIKGPENE
                     YMFTPMATGGAVVSQRLVQNNPEAAKNLIRAMNDAVDFMRTNDSESRQIIAKDTKLEA
                     NVAAKLDLLQLWKINETDLSGVQKYLDFLAETKILTKRIAAEDIYLNRSFIQ"
     gene            5426..>6025
                     /locus_tag="VGB76_02915"
     CDS             5426..>6025
                     /locus_tag="VGB76_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0097883.1"
                     /translation="MNAITVQNVQKSYLEGDKRILVLNDLTLSVGEHEILSLFGPNGC
                     GKTTLINICAGLMQADAGVVQIFGADPRDVRIGYIPQAFGESLFPWLTNLDNICFPLS
                     LDGLTKKRSREEAVRFLQQIDISLPLENYPYESSGGQKQLAALGRALVSSPSVLLADE
                     PFSALDYTTRLEIQDKFLKVVSESLQLATIVISHQIEDAI"
BASE COUNT         1370 a         1707 c         1627 g         1321 t
ORIGIN      
        1 atccagccgt acttcgagtt tgcgcatcgc cccgcctccc tcgcttgcgt gacggtcgtt
       61 ccgccctccg ctccagttcg gccagagacc tgactggcct gggggcgaag agctgatcaa
      121 agcccgcacg ggcctcgaag atgacggcga gtttcagcag gcgatccagc gagatgcgcc
      181 cgctacgctc gaagacccga taggtcggca gggcgatccc cgcgcgctcc gcgacctcct
      241 gctgagtcca cccgcgctcc agacgaagtg ccttgacccg acgggccaaa gcgcgcctaa
      301 tatcaagagg agtctgaagc tctatgggta acatattatc agttatcctt catctaccaa
      361 gctaactaat attattctat cagttagtaa tcgagcgtca accacgattt tcccctgccc
      421 attacaggat ttttctcgcc cccttacagg aatctcatgt cccgacattt gtccattttc
      481 gtcgcgtgtt cattgcacgt gaacacgcga cgaaaatgaa cacagacata aaaaggtggc
      541 gatataataa aaggcatgag ccgcgccgaa gagagactgc tcaacccgcc gccgggaggt
      601 agcatcgagg cggcgcgtgc ccacggcgtc gacctcagct tgctcgtcga gcgtctgcgc
      661 aaaaccccgg aggagcgcgt gcgcgacttg caacgggctg tcaaggttat agaggccgcg
      721 cgcggcaagg cgctcaaagc ttcgcgtgtt cggggcggcg cgattgattg actttgagca
      781 agcactgacg gcgctcgctc gagcccgcgt gcggttcgtc atcgtgggcg gcctcgccgt
      841 cacgatacac ggctcctcat acgtcacctt cgatctggat ttctgctacg cccgcgtccg
      901 cgagaacctg ccgcgactcg cgcaggccct cggcccctat catccgcggc cgcgcggagc
      961 gcgtcgaggg gtccgccacg agataggcct gtcccagctc gccgagcgcc gggggccaag
     1021 ggatgctccg gtagtcaaag cctcggtata acccgcctaa atcggcccgc cacagctgcc
     1081 gctatcagtt caaacttgcc acctatacgt ctttaatagt atccagatac gtattctgac
     1141 aaggacgtgc caccggcatg atcaaacaag taaccatcac cgatttgcag cggcgcgcag
     1201 ccgaggtcat cagcagtctt cgggaaggcc cggtcgtcgt taaccagcgc gggcgtccgg
     1261 tcgccgtcat ccttaccgcg gaggcttacg agcagatgga gcaggccgtg gccgaggtcg
     1321 aggccaagca ggtgcgcgag atcgtcgagg ccgggctctc gagttacggc gccggacgca
     1381 cctcacccca ggcggccgtt gcgcagcgcg tccgcgcctg ccgccggaag aagtgagcct
     1441 cacggttgtg tcgtcgtacg acgccattca ccgtttgcaa gtctcgcgcc ggttccgacg
     1501 aagagtcggt tggcctgaga cccagttcca tctaacggtg ggcaccagcc gccgccccga
     1561 gcgattcgat gtacatgaaa tctttcgtgt cggcggtcgg gtgcatgcgc ctgtcaggtc
     1621 gcggtgccga ggagtacttt caccggcgac actcctttca ccagaaccac accgagcgcc
     1681 acacagttga ctatgacggt gagccagtat cctatcagga acgaagtctt ccgcgttttg
     1741 tgtcgcaaaa gcacctgagc gatcagtgcg ccgggccagc ccccgaccag gctcatcaga
     1801 tgcaacgtcg actcggggac tcgccggccc gcattctggg cggccgtctt gtcccgggcg
     1861 tagacgccgt acgtgataat gctcgcgccg taatagagca acagccaact catctcgagc
     1921 cgccctgaaa ccacgagcgc ggttaccgcg gcgaggaaac tgattgcgaa cgtgatcggc
     1981 gcggagggaa tcccacgcgt tggcgaggcg ctacgtgtac ccacaacgaa tcggacatca
     2041 ttcgcctgag gccgtccatg cgaatcgaac gcgagcgtgt aactcaccgc ctcattcacg
     2101 cccggcctac gatcacttcg tgggaatgac gagatgtgaa caaatacgga gccgccccct
     2161 tcagaagggg agatgaatcc gaacccgcgc tcgtcgttcc actttgcgat tcttccctgg
     2221 tgacgcattt tctattttca agcgatctca agcccggcag cgcccgcccg ccagctccac
     2281 tgctattgat gacaggcgcg tatcgggggt tgggtacata ctgatgtcag gcggtcggcg
     2341 aggcgttatc acctatgtct ttatccgctc aggtaactgt tccgtcattc actcgctgtc
     2401 cactaaccgt cggaacatgc ccgggtagtc cagcacgatg ccgtcctcgt ccacgggaag
     2461 ctccaccgag aagccgctgc cgtcgaggct ctcgaagagg tagagccggt cggctagccg
     2521 cgtataagcc tgcggctgcg ggcggaccgt caggtccggc gcgaagaccc aggccatgcg
     2581 gaactgccgc ctctcgccga ccaccatcgg ctcgcggcgg acggggaagc tgttggtgaa
     2641 aggggtcggc cagatgtcaa tatccacgca gccgtcgagt tcgtcgatgg cccggccgtc
     2701 gtcgtctcgc caccggcccc ggccgtccgt tcgcagattg agtgatgtgg tgaagcgctc
     2761 cttcgtgaca accagatcgg cctcgcggag ccgccacaat tcgtcccagg tcaggcggta
     2821 ggtgagccgg aacgggccgc gcccctcgtc aaacgccagg acgacgctgt cggccacatg
     2881 ctcatccagg agcagatgct ccagccccac gccctcccgg cccttgttcc aggtgggcag
     2941 ccagcaaatc gttcgtctaa gcgctattgc aggcaaaagt cctctttata gtcaccaaga
     3001 tggaagacgc atgatcgaaa tttacccgga agggaagccg ccgggagggc ttaaccagac
     3061 gagccctcat ttaggggaaa gcaatctctt catagccgcc ggcgtgcagg ttgtctatgg
     3121 cctgggcgac ccgggtgacg tcacccctga tgcgcggcca gctcgtcgtc atcagcacga
     3181 gcacgccgat tctccggccc gccaagttct gttggtagcg gatgttctgg tccgtggtga
     3241 cgagggcgtc gaaaccttcg gcctcggcgc gggcgagcag ctccccgttc ttgaggtcgt
     3301 gccagcccct ctcgtatgca gtctcgacag tgtggtaagg caggtgattc ctcaggggca
     3361 cgggtgtccc ctgatcgaag agaagcctca tcgctaagcc gcctggcggc ccgggcgcag
     3421 actttcgatg gcgcactcga ggacggcctc gacctgctca cgggtgacgc cggggaacca
     3481 tgataggaag tcttcgaccg aggccccgcc ctcaaggttc tcgaagaggg cgacgacggg
     3541 gacgcgggtg ccgcggaaga cccacgagcc gctgaccttc tccggatcct gctcgacggc
     3601 agtgcacgta gaccaatcca tcatggctct attgtatcgc cggagccctg tttgttctag
     3661 acatttgtga ggtcacgccg cggaactgag cgaacccgag acggccactt cgagcgttaa
     3721 ccggactcct aaaggtaacc tgctctttcg aactcgaagc atgtgttttc cccgctccgc
     3781 ttacaggaac ccccgttcgg ggaacgggtc aagagagaat gtcttaagcc atttctaata
     3841 accaggaggc tgcaaaaggt gtagataaac tctagcgccg gcggcgcgct tgagggtcag
     3901 aaagaaacgc caaacttccc gtggtatctc aggaatgaaa taaaaagctt gagtttcaga
     3961 taggctagat atactatgcg attctgcaat agactttact gagcgactgc ctccatctcg
     4021 aattttcagt taagtatttc actcaagtcg ctgattctac ttagctagta ttcattttgt
     4081 cctcatgaac actcttagcc aacttttaac cttaagcatt ttaagcggta tccagaatca
     4141 aatactaagg acatatagct ctgctaggac caagggctaa agggccttaa tgagttctct
     4201 aagttatctc acttatatcg cggttcatta ggttaagagt acgggttgct ttatagtcct
     4261 taagtattcc tcgactcatt atcataaggc tcttagtaat tatcattgaa agcgggggca
     4321 gtagttgcct cattcctcgt cgacactggt gatagtcagc ggaggagtta gccattaatc
     4381 ttggcggata gctcgtatcc gtttagggag acttcacaat catgaaaaaa cggactatcg
     4441 gcatagttgt cattttcatc attctcatta tagctgtcat agcctaccaa atttactcgc
     4501 ctcataatgt tacgcaagag tccaccgtta tccgcattgg atacaagcct aattccggat
     4561 atcaacactt cttcatcgcc caggagaagg ggtactttaa aaagtacggg ctcaatgttg
     4621 agggcgtcac attcgaatct acaaatcaga tggttcaagc tgtagttaac ggtgatcttg
     4681 acgctacggc cgcgagttca atcgaggtga tcggactcgt cgaacagaat tcgcctaatt
     4741 tactgaagat atatctcact ctggtattcg ataaggatca tgctttccac tcggtactag
     4801 taccaccaga ttctaaaatt caaacactgg ccgatttgaa gggtaaaaag attgggaccg
     4861 ttcccggctc aacttctcaa tcgtggctgg aaataatcct cggccgtttc tttgattcca
     4921 aaaaggatgt tcagattatt cagctcgaac cgcgcctgca gttacaagcg ctgagttctg
     4981 gacaggtgga tgcgctctac acggtagacc ccattgtcgc gcaagctcaa gccaagggct
     5041 tggcccgggt gttgataaag gggccggaga acgaatacat gtttacccct atggctacag
     5101 ggggcgcagt agtctctcaa cggttagttc agaacaatcc agaggcagcc aagaacctca
     5161 taagggcgat gaatgatgct gtcgatttca tgagaactaa tgactccgaa tctcgacaga
     5221 ttattgctaa agataccaag ctcgaagcta acgtggccgc caaattggat ttactacaac
     5281 tgtggaaaat caacgaaact gatctctcgg gagtccaaaa atatctggac tttttagcag
     5341 aaacaaaaat tctcactaaa cgcattgcag cagaagatat ttacttaaat cgttccttta
     5401 ttcagtaatt gcccccttac actcgatgaa tgcaattact gttcaaaatg tccaaaagag
     5461 ttatttagaa ggtgataaga ggatattagt gttaaatgac ctgacgttaa gcgtcgggga
     5521 acatgaaatc ctttctcttt ttggtccgaa tggatgcggg aagaccactt tgattaatat
     5581 ctgtgctggc ctgatgcagg cggatgccgg agtagttcaa atctttggtg ctgacccaag
     5641 agatgtgaga attggctata tacctcaggc cttcggggag tcattatttc cctggttaac
     5701 gaatttagac aacatctgct ttccacttag cttggatggg cttactaaaa aaagatcgcg
     5761 agaggaagct gtcagatttc tacaacaaat tgatatcagc ctgccgctcg agaattaccc
     5821 ttacgaatca agtggggggc aaaagcaact tgcagcactc gggcgggctc tagtttcatc
     5881 cccttcagtt ctgttagcag acgagccctt ttcggcgctt gattacacaa cccggcttga
     5941 gattcaggat aaattcttaa aagtggtgtc agagagccta cagctcgcga caatcgttat
     6001 tagccatcag attgaagatg ccatt
//
LOCUS       DASWNS010000085        14496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_211627, whole genome shotgun sequence.
ACCESSION   DASWNS010000085 DASWNS010000000
VERSION     DASWNS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14496
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211627"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..1273
                     /locus_tag="VGB76_02920"
     CDS             392..1273
                     /locus_tag="VGB76_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEY0097884.1"
                     /translation="MRRFVVALFLTTLCASFALAQGQSAPTLRVVTEDPSLPSELFYG
                     DVRVKPIRLRPGTNTRITIDDSDFFVQQQYIDFLSRFPEAGGFAAWMRVLDSCNNDPN
                     CLHGVNGKRVLVSQSFFQSIEFQYKGGFVYRFYKASFNRLPTYGEIVEGMRSVTGQTE
                     AEVIAKRNAFMNNWVTRGDFATAFPASLSPADYVNKILQTAGVTVANKDQLIAGLTNG
                     TETRASVLGKIVESSELYTKEYDPAFVAMQYYGYLRRAPEPEGYNNWLTYLRNNPGDY
                     KTMVWGFVYSGEYRNRH"
     gene            1373..1765
                     /locus_tag="VGB76_02925"
     CDS             1373..1765
                     /locus_tag="VGB76_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0097885.1"
                     /translation="MKQTIAHIALVVDDYDEAIKFYTEKLDFTLVEDTPQSETKRWVL
                     VAPKGAEECCLLLARGVGEEQRSRIGNQTGGRVFLFLRTDDFWRDYENMGARGVNFVR
                     EPKTEEYGTVAVFADLYGNLWDLVEFRK"
     gene            complement(1793..2956)
                     /locus_tag="VGB76_02930"
     CDS             complement(1793..2956)
                     /locus_tag="VGB76_02930"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0097886.1"
                     /translation="MRIAIDGVPLAEPKTGIGHYTFELARGLAALAPEYDFELVAHVP
                     IEASVEDAFEESLPANLRTVHARVNSLSRRWWMIGLPLYVQQNGITLFHGTNYKIPLW
                     NRCRTVVTIHDLSLLLHSHTHEAELVRRARRRLPATARMASKIITDSESVKREICEHL
                     RVKPEKIAVVPLAPRRAFRPVAAQGAADARRRLGVEDEFILFVGTVEPRKNLLTLVRA
                     FEELTRRTGFRTQLVIAGQKGWLTEELFALIEQSGLGARILFTGYISDADLAALYSSC
                     RVCVYPSLYEGFGLPPLEAMSCGAPVVTSRIPVIMETVGEAARLVEPTNVRELADTIA
                     HVCGSEEERARLSSIGTVRAAEFTWERTARLTLDVYSELLPAMSPVKPRTARV"
     gene            complement(3263..5890)
                     /locus_tag="VGB76_02935"
     CDS             complement(3263..5890)
                     /locus_tag="VGB76_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097887.1"
                     /translation="MKNAFRLSLIALMLVCLSAGLAVAQTATPSPTPRYSSFTQVTSS
                     AANAFVGGISGDGRFVVFESTGDLATLLPEETPTTRSVTNTDGNREIFLYDYAQRRIF
                     QITNTTSARKDATKPALAVNTTTGLPDFSNVDVEVSNNRPFISRNGRWIVFSSNADTP
                     FTFDGDASRTALKADGNQEIFLYRIPAVETVDLRSGTDSGFVNLRVNDFTRVTDTTAS
                     RLPVPGTDVTSPIVADDNRAAQVNDRASRIVFTSTRNRFAPNGADFRNPDANPEIFIW
                     TRNTLIPATQPATGVFAQVTDTSGAFTFNDNPSISGDTSLSDETVELSTSMIAFVSNA
                     TAMRDINGTPFNTLANDDGNAEIFVAPVTLAGPTDGITQATRTQRANPGNLVNVLNPG
                     PRMSRDGRFVAFESVATNPAANTTANEVTRGLFVYVIQSNTFVQIGPRGLASANEEDV
                     LRFPVFAGSGAATQLVFTSALNFTPGGARLNLTDTTGMNPAGLKQIFSLPVPTTTAEA
                     QAFTRLTNTTISGNAALQPFVSNSIERIAFSFNGELGGGNGTSTSEGSGSAEVFYLNV
                     PPAAAPANDTPASASALAYFTGATEREVVTPAASPTPTPSPTPAATPITGLAPGMIGI
                     VRKTAASGAFTLASTTTNVCPPATACDVASEFQRRPPLPVELAGVSLSINNAAAGLYF
                     VSPTQINFVVPRGLAPLSGAATYPVVINVVEGGVSRTIRSVLQIVAAQPDIFSTTNGA
                     GGRAVVGNATNPLTVGLGMTEPFTVTTTYVNGSGQSVTEATRLRVLLTGVRGIPRSAI
                     TVRLIKADGTTTDIAPGDQIPTDPQVVDRMPGALTLDFRLPATLANAGDVSIIVIVNT
                     AGGTFMSRPVDTAPRFRIN"
     gene            complement(6188..9985)
                     /locus_tag="VGB76_02940"
     CDS             complement(6188..9985)
                     /locus_tag="VGB76_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0097888.1"
                     /translation="MFRSPLGRNLAVRVRATRRLALVLSLFALAAGLPYIKAGAWQQE
                     EERPRKSQIVAKGAANIVEGAEFVPGEVLVRFRTDAAAKSAEASPMSLQREVDGGEVS
                     FQRFEGSDMVRGLRLAKVEESRTLEALAELAERPDVLYAEPNYIWRARRTPNDPRFGE
                     QYGLNRINAPSAWDTTVGSRDVVVGVLDGGVDINHPDLRDNIWTNAAEANGAPGADDD
                     GNGYIDDVNGWDFDHNDRTVFDNQEGDDHATHVAGTVGARGNNAVGVTGVNWEVSLMS
                     LKVLGPEGGTVSNIIEGYNYALQMRQRGVNLRVLNNSYGGPGKSQAALDAIQQLNAAG
                     ILFVVAAGNGGGDNIGDDNFVFPDFPASYDVPNVLAVASTNSFDGLVSSSNFGARLVS
                     MAAPGQSILSTVPTFVNPSGYEYFGGTSMASPHVAGAAALILAAKPSITMSELRGVLA
                     YTGDRISPALDGRTTTGRRLNVGKALQSALENDTTPPAPVGNLRIQPTTSGVGRNISL
                     AWTATGDDGNSGTAADYDFFFVNPATGAKTLLPTSLNPAPAGTQQSIFVTMPYRNFEG
                     TLVLRAYDNAGNSSDTSLAVRIPDNYRTNPYNVTLSGAAGLTPITGTSLVTGDDKYVG
                     YTLPFAFRFYGESQPVTISTNGAIYFSQPPRRENQDADDAGSSVQGLQGQRMIAGLWD
                     DIDINTARRSDSGIFVRESDSNHIIFRWQGVPCNPSASTGGCTGGSPINFEIELRADG
                     TIQMRYGANASMFPVVGISNGEPDAYVVTTHTSETQRINLTNAATVTFTPRPRSLTIS
                     GRVTDNGVGLSGVPLTLSGTHSATTTTDAAGHYFFANLSIGASYTVTPSRPGSTFAPT
                     RAVFANNGLEELTADFAVSVAGPNSIGLSHPSYQYGEGDGRATFTVTRTGDSIGTSTV
                     DYRTTDSDTFTVGCADTVGNNGGAFARCDFATTSGRLTFAPGELNKTITVPLIDDGHD
                     ESAETFQIQLANASGSGTSLGAQSVATVTLQDNDTGVSPNPIITLAPGDYPFFVRQQY
                     LDFLSREPEANEPWTAVMARCANVNTGPATNTDCDRIAVSRAFFESPEFNLKGFSVFR
                     FYKVAFNRLPQYTEIVTDMSFVAGATAEEVFARKAQLTNTFVARPEFTNAYGAMSNAQ
                     FVSALLGRYSLASVTTPDPLTPDSGTRVNLTSADLTARLDNNSLTRAQVFRAIADSDQ
                     LQALEFNSAFVAMQYYGYLRRTPEPAGYEANLNALQRGTSRREMINGFLNSTEYRLRF
                     GQP"
     gene            complement(10219..12906)
                     /gene="lptD"
                     /locus_tag="VGB76_02945"
     CDS             complement(10219..12906)
                     /gene="lptD"
                     /locus_tag="VGB76_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY0097889.1"
                     /translation="MRSFFFSKNLRTNEKHLLLSWSAFVCLVVCGCAAPRAFGQQQQS
                     NPVERKVLNPITDTPNVNPLQQEQPIRPRMPVKSDGAATAGQSTERLDIDAGKQTASG
                     PENARVVVYEGDVDARVGIYRLQSDKLTVYEAQNRVVAEGNVVFDQGQSQRITGSRAE
                     FNYATKLGFFIDSTGFTNQTEDGTIIYFTADRVEKVAANRIVVHNTELTACDEETPKW
                     SFTARRAEITLNDRVRARNPKFKVKGMTLLPLPYASISIKRRDRASGFLTPTFSGSGQ
                     KGFRLSNAYYQTLGRAADVTFRNDIFSQRGLGFGADMRTRANSRSYFNLGFYTVKDRL
                     FGEKESAEHPDQGGSSFYMDGVHYFPNGFLAAADINITSNLAFRQVFSDNIQQAISPE
                     ERSQVFVNKNAGDYSFNFLARNQVTALPSARIRIRNLPSIELQKRPSPLSFFERLPVY
                     FSFEGGLEGVSRKETVDDLATFRLEAGGEPLITPSVVQRLDVNPTFTVPLYRDGWSAT
                     TTASLRGTYYSNSIDPLTRLVLGRDVSRAYAELRFDVRPPALARNFYRGDDSLRFRHV
                     VEPYVTYRKITGINNFPRIIRFDSTDAIADTNEIEYGIVNRFFKRHSTENVSGKTPLT
                     SRTTNGASNGAANDATNGAANDAASSSVTGGAANAATQAASGNGKTLAPVATRPEEVL
                     SITVRGKYFFDPYFGGALIPGRRNQFYPINTFSGFTYGGVPRRFSPLSVEARYRPHPN
                     LTTDVRTNLDVQGGGVRDLVVSFGLNRRLVQIFQTFYYTRAIKLAPSLQQFADARGRE
                     AGTLRGSQYSPYVFIGNRERGLFGGAAIFFDFQNRPGKTSPLVSSTLTLGYSWDCCAV
                     TLQDTFFNLGLRKENRVVFSFRLNGIGTFGTEQIGQNFR"
     gene            12911..13033
                     /locus_tag="VGB76_02950"
     CDS             12911..13033
                     /locus_tag="VGB76_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097890.1"
                     /translation="MSWKTSDALNHGARMRRFSHGNRRALSSLQKRGDANMRRQ"
     gene            complement(13117..13593)
                     /locus_tag="VGB76_02955"
     CDS             complement(13117..13593)
                     /locus_tag="VGB76_02955"
                     /inference="COORDINATES: protein motif:HMM:NF021516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HEY0097891.1"
                     /translation="MVAVEHDRRASVAGILLGIGLGGFVDGIVLHQILQWHNMVSNWI
                     PPTTMEAMSVNMLWDGIFHAAVWLVTLLGVFLLWSAAYRRAAIPSLRAFVGQLFLGWG
                     LFNLVEGIIDHQILGVHYVRQVPNYTIYNLTFLATGGVLFILLGWLLARAGQRVEG"
     gene            13832..>14496
                     /locus_tag="VGB76_02960"
     CDS             13832..>14496
                     /locus_tag="VGB76_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097892.1"
                     /translation="MSHTRACADQPASLLDQNIHAIPELARTGEESSGITGRRRPEAL
                     RPIANDRRLSPAANAVAIIQAMSGEASGNLRLMIVAGEASGDAHAASLVRALRESAPG
                     VRFQFFGATGREMRAAGVESVVATDDLAITGLMEIGRALKKFWRAYQTLRRVGIERAP
                     QAVILVDWPDFNLRLARFFHRRGLRVIYYISPQLWAWREHRARNIRRDVDLLLAILPF
                     EPS"
BASE COUNT         2719 a         4193 c         4817 g         2767 t
ORIGIN      
        1 tcgcttctcc gaccgggcgg gagtttcgct ctgaccggcg ttttatcccg tctaattccg
       61 cgcctaaacg ctttacgggc aacgcctttc cccgtttccg gcatctctgc tctgtccctc
      121 gcaaggcttt tggccgccgt cactttttcg tcgcgacgaa aacttatttt ccgcctgacg
      181 agtagcgtgc ggcgtctaaa cagcgcgtgg gttcaatatc gttgacaaac gttctgaggg
      241 caagctacca taacagttgt tgggctgtgc ttgcgagcca accgctcgac cgaagcgcag
      301 agagcgttcg agtagccccg gctctcccac aggaatcctt aagctacaaa aaaacagtcc
      361 caccgtctat agagtaccgg gagtaacaaa catgcgtaga ttcgtcgtag ccctattttt
      421 aaccaccctg tgcgcctctt ttgcgctcgc gcaaggccag agcgcaccca cgctcagagt
      481 cgtcaccgaa gacccttcgc tgccctctga actcttctac ggcgacgtgc gcgttaagcc
      541 catccgcctc cgaccgggca cgaacacacg catcacgatt gacgacagcg actttttcgt
      601 ccagcagcag tacatagact ttctgagccg cttccccgaa gccggcggct tcgccgcatg
      661 gatgcgcgtg ctcgatagtt gcaacaacga ccccaactgc ctgcacggcg tcaacggcaa
      721 gcgcgtgctc gtctcgcaga gtttcttcca gtcaatcgag tttcagtaca aaggcggctt
      781 cgtctatcgc ttctacaaag cgagtttcaa tcgcctgccg acttacggcg agatcgtgga
      841 agggatgcgc agcgtgaccg ggcagaccga ggccgaagtg atcgccaaac gcaacgcctt
      901 catgaacaac tgggtgacgc gcggcgactt tgccaccgct tttccggcga gcttgagtcc
      961 ggcggactac gtcaacaaaa ttctccagac ggcgggcgtc acggtggcga acaaagatca
     1021 actcatcgcc ggtttgacca acggcacgga gacgcgcgcc agcgtcctcg gcaagatcgt
     1081 cgagagcagc gaactctaca cgaaagagta cgaccccgct ttcgtcgcca tgcaatacta
     1141 cggctacctg cgccgcgcgc ccgaacccga aggctacaac aactggctca cctacctgcg
     1201 caacaatccc ggcgattaca aaacgatggt ctggggcttc gtctactccg gcgaatatcg
     1261 caaccgccat taagcgcagc acgcacgcga aacaacttcg aggccagcct ttcaacgggc
     1321 tggccttttc aatttaagcc tctacccgaa ccccgttcgc ccagacaaaa ccatgaaaca
     1381 aactattgcg catattgctt tagtcgtaga cgactatgac gaggccataa aattctatac
     1441 cgaaaaactg gatttcactc tggtcgaaga tactccgcag agcgagacga agcgttgggt
     1501 tttagttgcg cctaagggag cggaagagtg ctgcctgttg ctcgccagag gggtcggcga
     1561 agaacagaga agccgcatcg gaaaccagac gggggggcgc gtttttctgt tcctgcgcac
     1621 agatgatttc tggagagatt atgaaaacat gggggcgcgc ggcgtaaatt ttgtcaggga
     1681 accgaaaacg gaggagtacg gcacggtcgc cgtttttgca gatttgtacg gcaatctctg
     1741 ggatttggtc gagttccgca agtagagacg agcgaacgat attactgccc ggctagacac
     1801 gcgccgtgcg cggcttaacc ggcgacatcg cgggcaagag ttcgctgtaa acgtccagag
     1861 tcagccgcgc cgtgcgctcc caggtaaatt ccgccgcgcg cactgtgccg atggaagaga
     1921 gacgggcgcg ctcctcctcg ctgccgcaga cgtgtgcgat ggtgtctgcc agttccctca
     1981 cgttcgtcgg ttcgacgaga cgcgccgcct cgcccaccgt ctccatgatg acgggaatgc
     2041 ggctcgtgac gacgggtgcg ccgcaactca tcgcttcgag cggcggcagg ccgaagcctt
     2101 cgtacagcga cgggtagacg cagacgcggc aggaggaata gagcgcggcg aggtctgcat
     2161 cggagatgta gcccgtaaac agtatgcgcg cgcccagccc cgactgttcg atgagcgcga
     2221 agagttcttc cgtcaaccaa cccttctgac cggcgatgac gagttgcgtg cgaaagcccg
     2281 tgcggcgcgt gagttcctcg aaggcgcgca cgagcgtcaa cagatttttg cgcggctcga
     2341 ccgtcccgac gaacaagata aattcgtctt cgacgccgag cctgcgccgg gcatccgccg
     2401 ccccttgcgc cgcgacggga cgaaacgcac ggcgcggtgc gagcggcacg accgcgatct
     2461 tctccggctt aactctcagg tgctcgcaga tttcgcgctt gacgctctcg gagtcggtga
     2521 tgatcttcga ggccatgcgc gccgtggcag gcaggcggcg acgcgcgcgg cgcacgagtt
     2581 ccgcttcgtg cgtgtgcgaa tggagcagga gcgagagatc gtggatggtg acgacggtgc
     2641 ggcagcgatt ccagagcggg attttgtagt ttgtgccgtg aaagagtgtg atgccgttct
     2701 gctgcacgta gagcggcagg ccaatcatcc accagcggcg agagagggag ttgacgcgcg
     2761 cgtgcacggt gcgcaaattc gcgggcagag actcttcgaa cgcgtcctcg acagaggctt
     2821 cgatgggaac gtgtgcgacc aactcgaaat cgtattcggg agcgagagcc gcgagtccgc
     2881 gcgccagttc gaaagtgtaa tggccgatgc cggtcttcgg ctcggccagc ggaaccccgt
     2941 caattgcgat gcgcatgctg taaacgataa cgagcggcca cgcccggtac atggcgcgcg
     3001 aatctacttt tcagtcttat tacctgccgc gagattatag cccctgaacg gcggaggcac
     3061 ccagccgggt ttcgccggaa aggaacagaa cacttacgcc gcagagacgc agagaagggg
     3121 cgcagaggaa aacgatgcgg gatgaaggat gaaggatgaa cgaaagccaa tactgcttca
     3181 gttcatcctt catccttgat cgttcatcgt ttcttttctc tgcgcccctt ctctgcgatg
     3241 aagtcttcgc gtcgccctgc gcctagttga tgcggaagcg gggcgcggtg tctacggggc
     3301 gactcatgaa ggtgcctccg gcagtattga cgatcacgat gatggacacg tcgcccgcgt
     3361 tcgccagcgt ggcgggaagg cggaagtcga gcgtgagcgc gccgggcatg cggtcaacta
     3421 cctgcgggtc ggtcggtatc tgatcgccgg gcgcgatgtc cgtagtcgtg ccgtcggcct
     3481 tgatgagccg gacggtgatc gcgctgcgcg ggatgccgcg cacgcccgtc aacaggacgc
     3541 gcaacctcgt cgcctcggtg acgctttggc cgctgccgtt cacgtaggtc gtcgtcacgg
     3601 tgaaaggttc ggtcatgccc agcccgacgg tgagcgggtt ggtggcgttg ccgacgacgg
     3661 cgcgaccgcc cgcaccgttc gtcgtgctga agatgtccgg ttgcgcggcg acgatttgca
     3721 gcacgctgcg aatggttctg gacacgccgc cctcgacgac gttgatcacg accggataag
     3781 tggccgcacc tgagagcggg gcgagtccgc gcgggacgac gaagttaatc tgcgtcgggc
     3841 tgacgaagta aagaccggcg gcggcgttgt tgatggagag cgaaacgccc gcgagttcga
     3901 cgggaagcgg cggcctgcgc tggaactccg aggcgacatc gcaagcggtc gcgggcgggc
     3961 agacgttggt ggtggtcgag gcgagcgtga acgcgccgga agcggcggtc ttgcggacga
     4021 taccgatcat gccgggcgcg agtccggtga tgggagtcgc agccggggtc ggcgaaggcg
     4081 tgggcgtggg cgatgccgca ggcgtcacaa cttcgcgttc cgtagctccc gtgaaataag
     4141 cgagcgcgct cgcggaagcg ggcgtgtcgt tggcgggcgc ggcggcgggc gggacgttca
     4201 ggtaaaagac ttccgccgag cctgagcctt ccgacgtgga ggtgccgttg ccgccgccga
     4261 gttcgccgtt gaaactgaaa gcgatgcgct caatcgagtt gctgacgaag ggttgcagcg
     4321 cggcgttgcc gctgatggta gtgttcgtca ggcgcgtgaa agcctgcgcc tctgcggtcg
     4381 tcgtgggaac gggcagcgaa aaaatttgct tcaagcccgc agggttcatc cccgtggtgt
     4441 ccgtgagatt gaggcgcgcg ccgcccggcg tgaaattgag cgcggaggta aagacgagtt
     4501 gggtggccgc gccgctgccc gcgaagaccg gaaagcgcaa cacgtcctcc tcgttggcgg
     4561 aggctaagcc gcgaggcccg atctggacga aagtattgct ctggatgaca tagacgaaga
     4621 ggccgcgcgt gacttcattc gcagtggtgt ttgccgccgg gttggtggcg accgattcaa
     4681 acgccacgaa tcttccgtcg cggctcatac gcggccccgg attgagcacg ttgacgaggt
     4741 tgccggggtt cgcgcgttgc gtgcgcgtcg cctgcgtgat gccatcggtg ggtcccgcga
     4801 gggtcacggg ggcgacgaaa atttccgcgt tgccgtcgtc attggcgagg gtgttgaacg
     4861 gcgtgccgtt gatgtccctc atggcggtgg catttgagac gaaggcgatc atggaggtgg
     4921 agagttctac ggtttcgtcg ctaagcgagg tgtcgccgga gatgctcgga ttgtcgttaa
     4981 aggtaaacgc gccggaggtg tccgttacct gcgcgaagac gcccgtggcg ggctgcgtgg
     5041 cggggatgag cgtgtttcgc gtccagatga aaatttcggg gttggcgtcg gggttcctga
     5101 aatccgcccc gttcggcgcg aaacggttgc gcgtcgaagt gaagacgatg cgcgaggcgc
     5161 ggtcgttgac ctgtgcggcg cggttatcgt ctgcgacgat gggcgaggta acgtccgtgc
     5221 ccggcacggg caggcggctc gccgtcgtat cggttacgcg cgtgaaatca ttgacgcgga
     5281 gattgacgaa gccgctgtcc gtgccgcttc gcagatcgac ggtttcgacg gcggggatgc
     5341 gataaagaaa gatctcttga ttgccgtcgg ccttgagcgc agtcctgctc gcgtcgccgt
     5401 cgaaggtgaa cggggtgtcg gcgtttgaac tgaagacgat ccagcgtccg ttgcgactga
     5461 tgaaggggcg attgttgctc acttcgacat cgacgttgct gaaatcaggc aggccggtcg
     5521 tggtgttgac ggcgagcgcg ggtttggtcg cgtccttgcg tgcgctcgtc gtgttggtga
     5581 tctggaagat gcgccgttgc gcgtagtcgt agagaaaaat ttcgcggttg ccgtccgtgt
     5641 tggtgacgct tctggtggta ggagtctcct ccggcagcag ggttgcgaga tcgcccgtcg
     5701 attcaaagac gacgaaacgc ccgtcgccgc tgatgccgcc gacaaaagcg ttggccgccg
     5761 aagaagtgac ctgcgtgaag gaggaatagc ggggcgtcgg gctgggagtg gccgtctgcg
     5821 cgacggccaa gcctgccgac aggcagacga gcattaaagc tatcaaggac aggcgaaaag
     5881 cgtttttcac tggaaaatct ccaaatgagg ccgaaacagg ataagcgatg ttcatgaaat
     5941 ttcttgggac cttcattcgc cggacggcaa gctgccacga caggcgaacg ggaacgggca
     6001 ttttaagcgc aacgcccgta ttagagcaag ttaggctgac agttcctctg atgagacgcg
     6061 ggcggcgcac cgttggccgg agaaaagaga aaggatgaag gaggaacgat gaacgaggaa
     6121 tattaaagca ctcaagcgag ttaaggcttt cattcctcgt tcatcgttcc tccttcatcc
     6181 tttattttca gggttggccg aacctgagcc tgtactcggt cgagttgagg aagccgttga
     6241 tcatctcgcg ccggctcgtc ccgcgttgca gggcgttcag gttggcctcg tagcccgccg
     6301 gttccggcgt cctcctcagg tagccatagt actgcatggc gacgaacgcg ctgttgaatt
     6361 ctaacgcctg caactgatcc gagtcggcga tggcgcggaa cacctgcgca cgcgtcagcg
     6421 agttgttgtc gaggcgggca gtcaagtccg cgctcgtcag gttaactcgc gtcccgctgt
     6481 cgggtgtgag cgggtcgggc gtcgtcacgg aagcgagcga gtagcgcccg agcaaggccg
     6541 agacgaactg cgcgttggac attgcgccgt aggcgttcgt gaactcaggc cgcgcgacga
     6601 acgtgttcgt cagttgcgcc ttgcgtgcga aaacctcttc cgccgtcgct ccggcgacga
     6661 agctcatgtc cgtcacgatc tccgtgtact gcggcaggcg gttgaaggcc accttgtaga
     6721 agcggaagac ggagaagccc ttcaaattaa actcaggcga ctcgaagaag gcgcgcgaga
     6781 cggcgatgcg gtcgcaatcc gtgttcgtcg ccggacccgt gttcacgttc gcgcaacgcg
     6841 ccatcaccgc cgtccacggc tcattggcct ccggctcgcg cgagaggaag tcgagatact
     6901 gctggcgaac gaagaaggga tagtcgcccg gcgctaaggt gatgatcggg ttcggggaga
     6961 cgcccgtgtc gttgtcctgc aaggtgacgg tcgcgacgct ctgtgcgccg agcgacgtgc
     7021 ccgagccgga agcgttcgcc agttgaatct ggaaagtctc cgcgctttcg tcgtgcccgt
     7081 cgtcaatgag cggcacggtg atggtcttgt tgagttcgcc cggagcgaaa gtgagcctgc
     7141 cggacgtggt ggcgaagtcg cagcgcgcga acgcgccgcc gttattgccc accgtgtcag
     7201 cgcacccgac ggtgaaggtg tcgctgtcgg tcgtgcggta atcaaccgtg gaggtgccga
     7261 tgctgtcgcc cgtgcgcgtg acggtgaagg tcgcgcgccc gtcgccttcg ccgtattgat
     7321 aactcggatg cgataaaccg atgctgttcg gacccgccac gctgacggcg aagtcggctg
     7381 tcagttcctc cagaccgttg ttggcgaaaa ctgcgcgcgt gggcgcaaag gtagagccgg
     7441 gtcttgaggg agtgacggtg tagctggcgc cgatgctgag gttggcgaaa aagtagtgac
     7501 cggcggcatc ggtggtagta gtggcggagt gtgtgccgct caaagtgagc gggacgccgc
     7561 tcaagcctac gccgttatcc gtcacgcgcc cgctgatggt cagcgaacgg gggcgcggcg
     7621 tgaaggtcac ggtcgccgcg ttcgtcaggt tgatgcgctg cgtttcggac gtgtgcgtcg
     7681 taacgacata agcatcgggt tcgccgttcg agatgccgac gaccgggaac atgctcgcat
     7741 tcgcgccgta gcgcatctgg atggtgccat ctgcgcgcag ttcgatctcg aaattgatcg
     7801 gacttccgcc cgtacacccg ccggtgctcg cgctcggatt gcagggcacg ccctgccagc
     7861 ggaagatgat gtgattggag tcggactccc gcacgaagat gccgctatcg gaacgccgcg
     7921 ccgtgttgat gtcgatgtcg tcccacagcc cggcgatcat cctctggcct tgcagcccct
     7981 ggacggaact ccccgcgtcg tctgcatctt gattttcgcg gcggggcggc tgggagaagt
     8041 agatcgcgcc gttggtcgag atggtcacgg gttggctttc gccgtagaac ctgaaggcga
     8101 acggcagggt ataaccgacg tacttgtcgt cgcccgtcac cagagaggtt ccggtgatgg
     8161 gcgtgagtcc cgccgcgccg ctgagcgtca cgttgtaggg gttggtgcgg tagttgtccg
     8221 gaattctcac ggcgagggag gtgtcgctgc tgttgcccgc gttgtcgtag gcgcgcagga
     8281 cgagcgtgcc ctcgaagttt ctgtagggca tggtgacgaa gatgctctgc tgcgtgcccg
     8341 ccggggcggg gttgagcgag gtgggcagca gggtcttggc tcccgtcgcc gggttgacga
     8401 agaagaaatc atagtcggcg gcggtgcccg aattgccgtc gtcgccggtg gccgtccacg
     8461 ccagcgagat gttacgcccg actccgctcg tcgtaggttg aatgcgcaga ttgccgacgg
     8521 gcgcgggcgg ggtcgtgtcg ttctccagcg cgctctggag agctttgccg acgttcagac
     8581 ggcgacccgt cgtggttctc ccgtcgagcg cgggcgagat gcggtcgccc gtgtaggcga
     8641 gtacgccgcg cagttcggac atggtgatcg agggcttggc ggcgaggatg agcgcggcgg
     8701 ctccggcgac gtgcggcgaa gccatcgaag tgccgccgaa gtattcatac ccgctcggat
     8761 tcacaaacgt ggggacggtg ctgaggatgc tttggccggg ggcggccatg ctgacgaggc
     8821 gcgcgccgaa gttcgaggaa ctgacaaggc cgtcgaagct gttggtggaa gcgacggcga
     8881 gcacgttcgg cacgtcgtag ctggcgggga aatcggggaa gacgaagttg tcgtcgccga
     8941 tattatcgcc gccgccgttg cccgccgcga cgacgaagag gatgcccgcc gcgttgagtt
     9001 gctgaatggc gtcgagggcg gcctgtgatt tgccggggcc gccgtaagag ttgttgagca
     9061 cgcggaggtt gacgccgcgc tggcgcatct gaagcgcgta gttgtagccc tctatgatgt
     9121 tcgagacggt gccgccttcg ggaccgagca ccttgagcga catgaggctc acttcccagt
     9181 tgacgcccgt gacgccgacg gcgttgttgc ctctcgcgcc gaccgtcccg gcgacgtgcg
     9241 tggcgtgatc gtcgccctcc tgattatcga agacggttct gtcgttgtgg tcgaaatccc
     9301 agccgttgac atcgtctata tagccgttgc cgtcgtcgtc agcccccggc gcgccgttag
     9361 cttcggcggc gttcgtccag atgttgtcac gcaaatccgg gtgattgatg tcaacgccgc
     9421 cgtcgagcac gccgacgacc acgtcgcggc tgccgaccgt cgtgtcccac gcggacggag
     9481 cgttgatgcg gtttaagccg tactgttcgc cgaagcgcgg gtcgttgggc gtgcggcgtg
     9541 cgcgccagat gtagttcggt tcggcgtaga gcacgtccgg gcgttcggcg agttcggcga
     9601 gggcttcgag cgtgcgcgac tcttcgactt tagcgagacg caggccgcgc accatgtcgg
     9661 agccttcgaa gcgttgaaag ctcacttcgc cgccgtccac ctcgcgctgg agcgacatcg
     9721 gggatgcttc cgcgctcttg gctgccgcgt ccgtgcggaa acgcaccagc acttcgcccg
     9781 gcacaaactc cgcgccctcg acgatgttgg ccgctccttt agctacgatc tgcgacttgc
     9841 gtggccgctc ctcttcctgc tgccacgcgc ccgctttgat gtaggggagg cctgcggcga
     9901 gggcgaacaa gctcaagact aaagcgagac ggcgcgtggc gcgcacacga acggcgagat
     9961 tccggcctaa cggggaacga aacatctgtt agtctctcct gtattaaaaa cgcgcccgca
    10021 tgaaagatgt gtcagcgcgc gcttatgatt tcgtcaaaaa atggcctgaa aagtagaact
    10081 gagggggcgt ccggcgatag cctgtgttta aggacgatgc gaactccatt cagatgcgaa
    10141 aacggcctct aaaagatgta cgcgcgcata ttatttttcg gatgtttacg ggcgttttcg
    10201 ggcaaacacg ggcacgcgtt aacggaagtt ttgcccgatc tgttccgtgc cgaacgtgcc
    10261 gatgccgttc aagcggaaac tgaagacgac gcggttctct ttacgcaggc cgaggttgaa
    10321 aaaagtgtcc tgcaacgtca cggcgcagca gtcccaactg tagccgagcg tcagggtcga
    10381 actgacgagc ggggaggtct tgcccgggcg gttctggaaa tcgaagaaga tggccgcgcc
    10441 gccgaacaga ccgcgctcgc ggttgccgat gaaaacatag gggctgtatt gcgagccgcg
    10501 cagagtgccc gcttccctgc cgcgcgcgtc ggcaaactgt tgcagactcg gcgcgagttt
    10561 gatggcgcgc gtgtagtaga aggtctggaa gatctgcacg aggcggcggt tgagtccgaa
    10621 gctgacgacg agatcgcgca cgccgccgcc ctgcacgtcg aggttggtgc gcacgtcggt
    10681 cgtcaggttc gggtgcgggc gataacgcgc ttcgacgctc aggggcgaga agcgacgcgg
    10741 cacgccgccg taggtgaaac cggagaaagt gttgatcggg tagaactggt tgcgcctgcc
    10801 gggaatgagc gcgccgccga aatagggatc gaagaagtat ttgccgcgca ccgtgatgga
    10861 gagcacctct tcgggacgcg tcgcaacggg cgcgagagtc ttgccgttgc cgctcgcggc
    10921 ctgcgtcgca gcgttcgccg ccccgcccgt cacactacta ctggccgcat cgttcgccgc
    10981 accgttcgtc gcgtcgttcg ccgcaccgtt tgacgcgccg ttcgtcgtgc ggctcgtgag
    11041 cggcgtcttt ccgctgacgt tttcggtcga gtggcgttta aagaaacggt tgacgatgcc
    11101 gtattcgatc tcgttcgtgt cggcgatggc gtcggtcgag tcgaagcgga tgatgcgcgg
    11161 gaaattgttg atgccggtta ttttccggta ggtcacgtaa ggctcgacga cgtgacggaa
    11221 gcgcaggctg tcgtcgccgc gatagaagtt gcgcgcgagc gcgggcgggc gcacgtcgaa
    11281 gcgcagttcg gcgtaagcgc gcgacacgtc gcgaccgagc acgaggcgcg tcagcgggtc
    11341 aatcgagttg gaatagtatg tgccgcgcag ggaggccgtc gtcgtcgcag accagccatc
    11401 gcggtagagc ggaacagtga acgtcgggtt cacgtcgaga cgctgcacga ccgacggcgt
    11461 gatgagcggt tcgccgcccg cttcgagtcg aaaggtggcg aggtcgtcaa ccgtctcctt
    11521 gcggctgacg ccttcgaggc cgccttcgaa agagaaatag acggggagac gctcgaagaa
    11581 gctgaggggc gaaggccgct tctgcaattc gatgctcggc aggttgcgga tgcgtatgcg
    11641 cgcgctcggc agcgcggtca cctgattgcg cgcgaggaag ttgaaactgt agtcgcccgc
    11701 gttcttgttg acgaagacct gcgagcgttc ttcgggcgag atggcctgtt gaatgttatc
    11761 ggagaagacc tgccggaagg cgagattgga agtgatgttg atgtcggcgg cggcgagaaa
    11821 gccgttcgga aaataatgga cgccgtccat gtagaagctc gacccgccct ggtccggatg
    11881 ctccgcgctc tccttctcgc cgaagagtcg atccttgacg gtgtagaagc cgagattgaa
    11941 ataggagcgc gagttggcgc gcgtgcgcat gtccgcgccg aagccgaggc cgcgctgcga
    12001 gaagatgtcg tttctgaacg ttacgtcggc ggcgcgtccg agcgtttgat agtaagcgtt
    12061 cgagaggcgg aagcccttct ggccggagcc gctgaaggtc ggcgtcagga agcctgaagc
    12121 gcggtcgcgc cgcttgatcg agatggaggc gtaaggcagc ggcaggagcg tcatgccttt
    12181 gactttaaac ttcggattcc gcgcacgcac gcggtcgttc agagtgatct cggcgcgacg
    12241 agccgtgaaa ctccacttcg gcgtctcctc gtcgcaggcc gtcagttccg tgttgtggac
    12301 gacgatgcga ttggccgcaa ccttctccac gcggtcggcg gtgaaataga tgatcgtgcc
    12361 gtcttcggtc tggttggtga agcccgtgga gtctatgaag aagccgagct tggtcgcgta
    12421 gttgaactcg gcgcgcgaac ccgtgatgcg ctgcgactgg ccttgatcga agacgacgtt
    12481 gccttccgcg acgacgcggt tctgagcctc gtagacggtc agcttgtcgg actgcaagcg
    12541 atagatgccg acgcgcgcat ccacgtcgcc ttcatagacg acgacgcgcg cattctcggg
    12601 gccgctcgcc gtctgcttgc ccgcatctat gtcgagcctt tcggttgact gccccgccgt
    12661 cgccgcaccg tcgctcttaa cgggcatgcg cgggcggatc ggctgctcct gttgcagcgg
    12721 gttgacgttc ggcgtgtccg tgatcggatt gagcactttg cgctcgaccg gattcgactg
    12781 ttgctgctgc ccgaaagcgc gcggcgcagc gcagccgcag acgacgaggc agacgaaggc
    12841 agaccacgac aggagcagat gcttctcgtt tgtacgaaga tttttactga agaagaagga
    12901 acgcaaaagt gtgagctgga agacctccga cgccttgaat cacggggcgc gcatgcgccg
    12961 cttttcgcac ggcaacaggc gcgcgctttc gtcgctgcaa aagcgcggcg atgctaacat
    13021 gcgccgccaa tgagcgacaa gcaaacagca ctctcagcct gtggcaggga gaaccggggg
    13081 cgtccgctcc gctcgcggac gccccgcctg ctcagtctaa ccctcgactc tctggcccgc
    13141 acgcgccagt aaccagccta aaaggatgaa gagcacgccg ccggtggcaa ggaacgtgag
    13201 gttatagatc gtatagttcg gcacttgcct gacgtagtgg acgccgagaa tctggtggtc
    13261 gatgatgccc tcgaccagat tgaacaagcc ccagcccaga aacagttggc cgacgaaggc
    13321 tctcagagac gggatcgcag cgcggcgata agctgcgctc cagagcagaa agacgccgag
    13381 caaagttacc aaccagacgg cggcgtggaa gatgccgtcc cagagcatgt tgacgctcat
    13441 cgcctccatc gtcgtcggcg gaatccagtt tgacaccatg ttgtgccact gcaatatctg
    13501 gtggagcacg atgccgtcaa caaacccgcc gaggccgatg cctaacaaaa tccccgcgac
    13561 cgacgctctc ctgtcatgtt cgactgcgac catccgagcc tcctctcaca gttcgttcca
    13621 agttttagcc ggagcgttgc attatggctc ccgccgcaac ctgcaatcaa cagagaagcg
    13681 gcgaaacaaa gcaggccgga caacgggcgg cctatccatc aacccgcgct attgactcac
    13741 tcccgcacaa tcgcccgtcg cacggacaat ctaactgtcg cacggacgac ttaacacgag
    13801 cgtccccatc taactcaagg acgccgggca gatgagccac acgcgtgcgt gcgccgacca
    13861 gcccgcaagc cttctcgatc agaatatcca cgccatcccg gaacttgccc ggacaggcga
    13921 agagagttcc ggcatcacgg gcagacggcg accggaggct ctacgcccta tcgcaaacga
    13981 ccgccgcctg tcgcctgccg ccaacgcagt tgctattatc caagcaatga gcggcgaggc
    14041 ttccgggaat ttgcgtctga tgatcgtggc gggcgaggct tcgggcgacg cgcacgcggc
    14101 gtcgctcgtg cgtgcgctca gagagagtgc gccgggcgtg aggttccaat ttttcggggc
    14161 gacggggaga gagatgcgcg ccgccggagt cgagtccgtc gtggcgacgg acgatctggc
    14221 gatcacgggg ctgatggaga tcgggcgcgc gctcaaaaag ttctggcgcg cgtatcaaac
    14281 gctccggcga gtcggcatcg agcgcgcgcc tcaggcagtg atcctcgtcg attggcctga
    14341 cttcaacctg cgtctcgcgc gcttcttcca ccggcgcggc ctgcgcgtga tctactacat
    14401 cagcccgcaa ctctgggcgt ggcgcgagca tcgcgcgcgc aacatccggc gcgacgttga
    14461 cctgctgctc gccatcctgc ccttcgaacc ctcctg
//
LOCUS       DASWNS010000086        11548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_444761, whole genome shotgun sequence.
ACCESSION   DASWNS010000086 DASWNS010000000
VERSION     DASWNS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11548
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444761"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VGB76_02965"
     CDS             <1..1167
                     /locus_tag="VGB76_02965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0097893.1"
                     /translation="MNALRTPTPLPRVPTPETPRRARESADEPPSPLPLAPARWDWSG
                     VRRVLVVRLRSIGDTVLATPALHALRRFLPDAQIDLLLEDWVAPLLDGNTDASRIITV
                     RRGSTLARLSLVRRLRAARYDVAYNLHGGTTAALLTRASGARFRVGYASYKFSALHNH
                     AAPPASELWRQEKTHSVEQQLALPGWTGVPVTDRPPTRLSIKDEADAKIVERLRAAGF
                     DVRAPQPFALFHPAAAFDSKRWDVRNFARVAERLDARGFAVVAVAAHGEREVVRAFAS
                     SAKARVASFNDLTLPEVAALAARARLFVGNDSGIAHIAAAVGTPSVVVFGSSNVAHWR
                     PWSTAPAEVVREEMPCAPCAGYTCGEFAEAQCIRRVTVERVEAAIGRVLAQIEG"
     gene            1212..1490
                     /locus_tag="VGB76_02970"
     CDS             1212..1490
                     /locus_tag="VGB76_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097894.1"
                     /translation="MGAAAGAAVAAAIANAIKSSGVLVTVTPEDFAAILRRTQQPLIV
                     AAQGGFFSTHYKYLTSYKGLAFYTKSPTPLVLPLGAEVVNAKGISIPQ"
     gene            1548..2546
                     /gene="rfaE1"
                     /locus_tag="VGB76_02975"
     CDS             1548..2546
                     /gene="rfaE1"
                     /locus_tag="VGB76_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEY0097895.1"
                     /translation="MELITAQRAAELAANFGGRRIVVYGDVMLDEFVWGDVSRISPEA
                     PVPVVDIRRESVRLGGAANVLANLNALGARASVVGVVGRDRAGERLLGALEEAGALNS
                     HGGLVTDESRPTTLKTRIIAHNQLVVRADRERRVPADAATEDRLVATLTAALQDADAF
                     VVSDYDKGAVTPRVLAEILPRATKRGIPVLVDPKIRNFDSYRPATLVTPNHHEALRLT
                     NTEDDTDEGIAAAARLIRQRLDCESVLITRGEHGMMLLEGDAAPVYVATVAREVYDVT
                     GAGDTVIATLAAALAARASLVEAAALANHAAGIVVAKLGTATASADELVNSFKLKD"
     gene            2616..4409
                     /gene="dacB"
                     /locus_tag="VGB76_02980"
     CDS             2616..4409
                     /gene="dacB"
                     /locus_tag="VGB76_02980"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY0097896.1"
                     /translation="MKKISAARLLSKLLLCALVLNPTAPGTLAQEAQRNRRVNQPPAP
                     RATPQPTPTPQAAPPQEAAPPQATPTPQATPAPQTTPTPQATPVLPAPRTIEVLRARI
                     HEVLRQPALAPAIFAIKVVSLDTGRVLFEENAEKLLMPASNMKLYTVAAALDRLGPDF
                     RFHTSAYAPEPPDNKGKVRGDLIIYGRGDPTFATRFNGVGSTDYLKGIDELAANIYAA
                     GVRRVEGDLVGDESYFTGAPRSPGWEWDDLQWYYGADVSALTVNDNALDLSVKPGAKV
                     GDPCVVTTGPNIAHIVNANLPRHSGVPPVPPSSAASDLAPVSIINGTTTTARASKREL
                     VIDRPLGQHGFFVSGSLPLDDPGYTASISVPRPAYAFITMLRAALERRGVSVRGRTSV
                     VNAEMRRAAPLDPAKLFEVAKRESPPFSEIAAQTLKPSQNLYTELILRTLGKRFPPTD
                     ARLTSAQAGISVVRAFLNEAGVNTSHLSLVDGSGLARQNLITAESTIQLLTYMSQHRY
                     AQVYRDAQPIAGVDGTLRGRMRNTVAAGNLRAKTGTLSNVSGLSGYLTSAAGERLVFS
                     MIVNHYPDDQPPRQHYMDAIAILLASFAGKT"
     gene            4530..5414
                     /gene="dapF"
                     /locus_tag="VGB76_02985"
     CDS             4530..5414
                     /gene="dapF"
                     /locus_tag="VGB76_02985"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY0097897.1"
                     /translation="MRFVKFHGYGNDYIVCEARELAGVGDVPEFVRRACDRHYGVGAD
                     GIATLGRCDADAGVDVDGGAAADFVLRIFNPDGSEAAMSGNGSRSAASFLYFQNLWQR
                     EELRLATKAGVKLYRLLERDARGRFRFAAEIGRPAFDSATIPFHTGEARERVVDFPLE
                     LRTGEIVRLTALQMCNPNACVFVDDFAHADWRRIGREIESHPQFPQRTNVEFVRVRDR
                     AHVEVRVYERGVGPTLSSGTGACASAVAACINNLTERRVSVEMEGGRLEVEWRDDGEV
                     LLTGEAEVIYRGEWLKDK"
     gene            5890..6465
                     /locus_tag="VGB76_02990"
     CDS             5890..6465
                     /locus_tag="VGB76_02990"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEY0097898.1"
                     /translation="MTFNAQTLVAENKLNAARNSQTQAVKTDSTLAILAHESGTLSGS
                     IKAALPIIALAGISQEAEEPADATVTTTTAPVEPSAANPAGSNALEPMQKFTATAYAL
                     PGRTASGQTVRRGLIAADKSLPLGTRVRLDAGRYSGEYLVADRGSRVRGRTIDIWVPS
                     NGEAIRFGRRPVKLTILSYGAKKAKGAGKTR"
     gene            6660..7706
                     /locus_tag="VGB76_02995"
     CDS             6660..7706
                     /locus_tag="VGB76_02995"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY0097899.1"
                     /translation="MTSDNIPTPAPLVEVRRGAITESRHRGHVVAVDGDGLIVAQLGA
                     PETVTYLRSSAKPLQAVPLISTGAADRFGFTPQEIAVACGSHSGESVHEATVEAMLRK
                     IGLDGGALKCGVHEPFDKEVALELRARGEQPRVLQNNCSGKHTGMLALALHLGGATET
                     YDQPANPAQLAIARTVAEFSGVPLEEIAVGTDGCGVPVFGITVRAMALAYARLVAPPA
                     AFDAKTRDACVRIVVAMRAHPELVGGTRQRLDTELMRAAQGVISKVGAEGVYTVGVEP
                     SARWPRGLGLALKIEDGEDRRARPTVVIESLRQLGVLDDATFGELAPYAKFDVRNHRG
                     DLVGEVRASFELEQ"
     gene            complement(8356..9552)
                     /locus_tag="VGB76_03000"
     CDS             complement(8356..9552)
                     /locus_tag="VGB76_03000"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HEY0097900.1"
                     /translation="MRVPFINRLILVGSLFSLLFASVYASPTFADAAKLLPNKIGEAT
                     ARGAAGLPRHAFFDPLKQENFAAVRSATRSYVSADGSEFSIQLFKTQSDSEAYALLTN
                     IRLAQASAPSSPVAHSSPVTQLNGVGTAAISLDGRVLFFKGAAVASVYGGSANDNYAS
                     LVTFARSFAETLEGGSGEIPVLVKHLPDWETAQERAVYVVTQPALREVVPNQPVLDAI
                     SFDGGTEAVTATYEGGARLVIVEYATAQFAFDANAAINARLSELRGAGQPVPSAYRRV
                     GNYAVFVFGAPDESAAAGLIDKVKYEQEVRWLGENPYATERANRAWLNMSTSVIVNTV
                     KATGLAIVICLSIGGIFGGWIFARRRAQAALTEQFSDAGGMLRLNLDEMSQQTNAARL
                     LGQGDK"
     gene            complement(9714..10406)
                     /locus_tag="VGB76_03005"
     CDS             complement(9714..10406)
                     /locus_tag="VGB76_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0097901.1"
                     /translation="MYCMNCGADIPANVRFCGSCGAPADTEATRVASRGGTGEATAGV
                     RTSFDSGITAVSRQPAQPLGEHAVRTPATHTTRDEADAERVIFSVRPTFLFIGIGYIL
                     AAVAAVGLSVLLAMFAGQYISAPIAVLITLPLLLIPAYKHLRRNTIKYTLTDSKIEID
                     QGFLARKTRNIPLRNIQDVTVSTTIPQRIFGFGDLVIENANEVGGQTLLDNIPDPRRH
                     AELLLRELRRWN"
     gene            complement(10420..>11548)
                     /locus_tag="VGB76_03010"
     CDS             complement(10420..>11548)
                     /locus_tag="VGB76_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0097902.1"
                     /translation="LQLLEAVRADIDAGAGGTLALIASAPTQASLETEAGATMPQQQA
                     RAFAEAHKFFRLNLRRVDSVAFYGPTLLLAYLPQKTRGAAQKEIARLSEEFQATQERA
                     DGSLEVGLASFPSDGTEIESLIERAEAALQQVRPAHAGEDEEETSPNARPQSKPPAND
                     ASTTLPPLVSRDAKPPVAPETPTAGRQARAAGELSPSRAVRESKQMDMETSMMPAGGA
                     GGGGVGQGTLAQAAADAAARERERRTRGAIMPRRLLLTVSDPARMAQINLLLRSAGYE
                     VRAAFDGLQAINLLRIERPDLLVIDVALYGIDGLEALRRLQRQHHGRLPLPIVLLLPA
                     SSTERERVRAEASELGVSSFVAVPYDPEELLTCVRTVGSTD"
BASE COUNT         2148 a         3658 c         3642 g         2100 t
ORIGIN      
        1 atgaacgctt tacgaactcc gacgcctttg ccgcgcgtgc cgacgccgga gactccccgg
       61 cgcgcgcgtg agtcggcgga tgagccgcca tcgccgctgc cgctcgctcc ggcgcgttgg
      121 gattggagtg gggtgcgccg cgtgctcgtc gtgcgcctgc gctccatcgg cgacacggtg
      181 ctcgccacgc ccgcgctcca cgcgctcagg cgttttcttc ccgacgcgca gatcgatctc
      241 ctgctcgaag attgggttgc gccgctgctc gacggcaaca cagacgcttc ccgcatcatc
      301 accgtcagac gcgggagcac gctcgcgcgc ctctcgctcg tccgccgtct tcgcgccgcg
      361 cgctacgatg tcgcttacaa tctgcacggc ggcacgacag ccgcgctctt gacgcgtgcc
      421 tccggcgcac gcttccgcgt cggttatgcg agctacaagt tcagcgcatt gcataatcac
      481 gccgcgccgc ccgcctccga gttgtggcga caggagaaga cgcactcggt cgagcagcaa
      541 ctcgcgctcc ccggttggac gggcgtgccc gtcacagacc gaccgcccac gcgcctctcc
      601 atcaaggacg aagccgacgc gaagatcgtc gaacggttgc gcgcggcggg cttcgacgtg
      661 cgcgctccgc aacccttcgc gctgtttcac ccggcagccg ccttcgactc gaagcgttgg
      721 gacgtgcgca acttcgcgcg cgtggccgaa cggctcgacg cgcgcggctt cgccgtcgtg
      781 gccgtcgccg cgcacgggga gagggaggtc gtccgcgcct tcgcgtcgag cgcgaaggcg
      841 cgcgtcgcgt ccttcaacga tctgacgctg ccggaagtgg ccgcgctcgc cgcgcgtgcg
      901 cgcctgtttg tcggcaacga tagcggcatc gcgcacatcg ccgccgccgt cggcacgccc
      961 tcggtcgtcg tcttcggttc gtcaaacgtc gcacactggc gtccgtggtc aactgcgccg
     1021 gcggaggtcg tccgcgaaga gatgccctgc gcgccgtgcg cgggttacac gtgcggggag
     1081 tttgcggaag cgcaatgcat caggcgcgtt acggtcgagc gcgtggaggc ggccatcggg
     1141 cgcgtgctcg cgcaaataga gggctgacga ttttccgcgc gccggagagt gtcgaaggga
     1201 gaacttactt tatgggcgca gcagccgggg cggcagtggc ggcagccatc gccaatgcga
     1261 tcaaatcgag cggcgtgctg gtgacggtca cgccggaaga tttcgcggcc atcctgcgcc
     1321 gcacacagca accgctcatc gtggcggcgc agggcggatt cttttccacc cactacaagt
     1381 atctgacgag ctacaaggga ctcgccttct atacgaagtc gccgacgccg ctcgtcctgc
     1441 cgttaggcgc agaggtcgtc aacgcgaaag gcatctcgat cccgcagtag tcgtcgcgtt
     1501 tgatcgtgta gaatctttta gtcacctgtc tttgctgacc gaaacggatg gaactgatca
     1561 cagcacaacg cgcggctgag ttggccgcga acttcggcgg gcggcgcatc gtcgtctacg
     1621 gcgacgtgat gctcgacgaa ttcgtctggg gcgacgtgtc gcgcatctcg ccggaagcgc
     1681 ccgtgcccgt cgtcgacatt cgccgcgagt cggtgcggct cggcggcgcg gccaacgtgc
     1741 tcgcaaattt gaacgcgctc ggtgcgcgtg cctcggtggt cggcgtcgtg ggacgcgacc
     1801 gcgcgggcga acgcctgctc ggcgcgctcg aagaagcggg cgcgctcaat tcacacggcg
     1861 gcctcgtcac agacgagagc cgcccgacga ctctcaagac ccgcatcatc gcccacaacc
     1921 aactcgtcgt gcgcgcagac cgcgagcggc gcgtgcccgc cgatgccgcc acggaagacc
     1981 ggctcgtcgc cacgctcacc gccgccctgc aagacgcaga cgccttcgtc gtctccgact
     2041 acgacaaggg cgcggtcacg ccgcgcgtcc tcgcggagat tttgccgcgc gctacgaaac
     2101 gcgggattcc cgtcctcgtc gatccgaaga ttcgcaactt cgactcgtat cgtccggcta
     2161 cgctcgtcac gccgaaccat cacgaagccc tgcgcctgac gaacaccgaa gacgacacgg
     2221 acgaaggcat agccgccgcc gcgcgcctca tacgccaacg tctggactgc gaatccgtgc
     2281 tcatcacgcg cggcgagcac ggcatgatgc ttttggaagg cgacgccgcg cccgtctatg
     2341 tcgccacggt cgcacgcgaa gtctatgacg tgacgggcgc gggcgacacc gtgatcgcga
     2401 cgctcgccgc cgcgctcgcc gcacgcgcgt cgctcgtcga agccgccgcg ctcgccaacc
     2461 acgccgccgg aatcgtcgtc gccaaactcg gcacggccac cgccagcgcg gacgaactcg
     2521 tcaactcctt caagttaaaa gactgacaag ttagaagact ctctttgagc gaacccgaag
     2581 actgaacggt tcgagcctct cgataaatta tgccattgaa gaaaatttcc gccgcgcgcc
     2641 tgctctcaaa attgctgctg tgcgcgctcg tcctgaaccc gacagccccc ggcacgctcg
     2701 cgcaggaggc gcaacgcaac cgcagggtca accagcctcc cgcgccgcgc gcaactccgc
     2761 agccgactcc gacgccgcag gccgccccgc cgcaagaagc cgctccgccg caagcgactc
     2821 cgacgccaca ggcgactccc gcgccgcaaa cgacgccgac gccgcaagcc acgcccgttc
     2881 tgcccgcgcc gcgtaccatc gaagtgctgc gcgcgcgcat ccacgaggtc ttgcgccagc
     2941 ccgcgcttgc gcccgcgata ttcgcgatca aagtcgtctc gctcgacacg gggcgcgtcc
     3001 tcttcgaaga gaacgcggaa aaacttctga tgcccgcgtc gaacatgaaa ctctacacgg
     3061 tcgccgccgc gctcgaccgc ctcggtcccg actttcgttt ccacacctcc gcctacgcgc
     3121 cggagccgcc cgacaataaa ggcaaagttc gcggcgatct catcatctac gggcgcggcg
     3181 acccgacctt cgccacgcgc ttcaacggcg tcggcagcac cgactaccta aaggggattg
     3241 acgaactggc ggcgaacatc tacgcggcgg gcgtccggcg tgtggagggc gacctcgtcg
     3301 gcgatgaaag ctacttcacg ggtgcgccgc gctcgcccgg ctgggagtgg gacgatctac
     3361 agtggtacta cggcgcagac gtgtccgcgc tcacggtcaa cgacaacgcg ctcgatctct
     3421 ccgtcaaacc gggcgcgaag gtcggcgacc cctgtgtggt tacgacgggt ccaaacatcg
     3481 cacacatcgt caacgcgaat ttaccgcgac actccggcgt gccgcccgtc cccccttcgt
     3541 ctgcggcgtc cgacctcgcg cccgtctcga tcatcaacgg cacgaccacg acggcgcgtg
     3601 cgtcgaaaag ggaactcgtc attgatcgcc cgctcggtca acacgggttt ttcgtcagcg
     3661 ggagtttgcc gctcgacgat ccgggttaca cggccagcat ctccgtgccg cgccccgcct
     3721 acgccttcat cacgatgctg cgtgccgcgc ttgagcggcg cggcgtgagc gtgcgcggca
     3781 gaacgtcggt cgtgaatgcg gagatgcgcc gcgccgcgcc gctcgacccg gcgaaactct
     3841 tcgaagtggc gaagcgcgag tcgccgccct tctccgagat cgcggcgcag actttgaagc
     3901 cgagccagaa cctctacacc gaactcatcc tccgcacgct cggcaaacga ttccccccga
     3961 ccgatgcgag actgacgagc gcgcaggcgg gcatcagcgt cgtgcgcgcc tttctgaacg
     4021 aagcgggcgt caacaccagc cacctctcgc tcgtggacgg ctcagggctg gcgcggcaaa
     4081 acctcatcac ggcggagtca accatccaac tcctgaccta catgagccag catcgctacg
     4141 cgcaggtcta tcgtgacgca cagcccatcg ctggcgtgga cggcacgctg cgcgggcgca
     4201 tgcgcaacac cgtcgccgcc ggaaatttgc gcgccaagac cggcacgctc tcaaacgtct
     4261 ccggcctctc cggctacctg acgagcgcgg cgggcgaacg cctcgtcttc tcgatgatcg
     4321 tcaaccatta cccggacgac cagccgccac gccaacatta catggacgcc atcgccatcc
     4381 tcctcgcttc gttcgcggga aagacttaaa gacagtgacg agtgacaagt gacgagatta
     4441 agggcaagtc taaagacagg attgctgctt taacttgtca cccgtcactc gtcactcgtc
     4501 actgtttttg gcacttggca ctgaatctta tgcgattcgt taaatttcac ggttacggca
     4561 acgattacat cgtgtgcgag gcgagggagt tggcgggcgt cggcgacgtg ccggagttcg
     4621 tgcggcgcgc gtgcgatagg cattacggcg tgggggcgga tggcatcgcg acgctcggac
     4681 gatgcgacgc ggacgcgggt gtggatgttg acggcggcgc ggcggcggat ttcgtcctgc
     4741 gcatcttcaa ccctgacggc agcgaggcgg ccatgtcggg caacggctcg cgctcggcgg
     4801 cttcgtttct ctacttccag aatttgtggc agcgcgagga gttgcgactc gcgacgaagg
     4861 cgggcgtcaa actttatcgc ctgctcgaac gagacgcgcg cggtcgtttt cgcttcgcgg
     4921 cggagatcgg gcgacccgct ttcgacagcg cgacgatccc gtttcatacg ggcgaagcgc
     4981 gcgaacgcgt cgtggatttc ccactggagt tacgtacggg cgagatcgtg cgcctgacgg
     5041 ccttgcagat gtgcaacccg aacgcctgcg tgtttgttga cgatttcgcg cacgccgact
     5101 ggcggcgaat cgggcgggag atcgaatcgc acccgcagtt tccgcaaaga accaacgttg
     5161 aattcgtgcg cgtgcgcgac cgcgcgcacg tcgaagtgcg cgtctatgaa cgcggcgtcg
     5221 gacccacgct ctcttcgggc acgggcgcgt gcgcctctgc cgtcgccgcg tgcatcaaca
     5281 acctgacgga gcggcgcgtc agcgtagaga tggaaggcgg acggcttgaa gtcgaatggc
     5341 gcgacgacgg cgaagtgctg ctcacgggcg aggccgaagt tatctatcgc ggcgaatggc
     5401 tgaaagataa atgatgaagg aggaacgatg aacgaggaat gaaaagcagg agtcggaaaa
     5461 cagaatacag gagtcagaat aaaacgcgcg cgtctcttta ttctgactcc tgtattctgt
     5521 tttctgaatt ctagttcctc gttcatcgtt ttcttccgcc gacatctatc acaaatttgt
     5581 gttctcgcgg ctggacagtc gttagaggct tgtgttagcc tcatttacga aacaagcgga
     5641 cagtgatgtt cgcttgtttt tctttttaag taacgatctt ttcggcgtgg ccgcatcaag
     5701 gatgtgatcc ccactcgggc tgacggttat cgtgacctcc tagagccaga ggtcgagtcc
     5761 tttcaaacct cgtgcaagga ctcgcgcggc gtcgaaaagt cttccatcgg aacaggagaa
     5821 ccgaaaaaaa tgctgaaatc cctctttgga gggggcgctg tgttaacagc cgcgctcctt
     5881 ctgtgttctt tgacttttaa cgcgcaaacc ttagtcgcag aaaacaaact taacgccgca
     5941 cgaaactctc aaacgcaggc cgtaaagaca gattcaacgc ttgctatcct cgcacacgaa
     6001 agcggcacgc tctccggctc catcaaggcc gcactgccca tcatcgccct cgccggaatt
     6061 tcccaagaag cggaagaacc cgccgacgca actgtcacta caactactgc gcccgtcgag
     6121 ccttccgccg cgaatcccgc cggaagcaac gcgctcgaac cgatgcagaa atttacggcg
     6181 accgcctacg ctctgcccgg tcgcacggcg agcggccaga cggtgcgacg cggattgatc
     6241 gccgccgata aatctctgcc gctcggcacg cgcgtccgtc tcgacgccgg acgctacagc
     6301 ggcgaatatc tggtggccga ccgtggctcg cgcgtgcgcg ggcgcaccat agacatctgg
     6361 gttcccagca acggcgaggc cattcgtttc ggtcgtcgtc ccgtcaagct cacgattctc
     6421 agctacggcg cgaagaaagc caaaggcgcg ggaaagacgc gttagacgct cgtagaagca
     6481 tcaaactgaa tagcagagag gcaaaggccg ctgtcgtcac tggcagcggc cttcgccttt
     6541 tcagactcgg acgtggcgag gcgggttgcg acgggcgaag tggcgcggtt aagatgcgtc
     6601 acaacaaaag caatggaaaa tttgagacag cctgttgagc gcgcccgccc gaacaagaga
     6661 tgacatctga caatatcccg acgcccgcgc cgctcgtcga agttaggcgg ggtgcgatca
     6721 cggaatcgcg tcatcgcggc cacgtcgtcg cggttgacgg cgacggcctg atcgtcgctc
     6781 aactcggcgc acccgaaacc gtcacctacc tgcgttcttc tgcgaagccc ttgcaggccg
     6841 tcccgctcat ttcgacgggc gcagccgacc gcttcggctt caccccgcaa gagatcgccg
     6901 tcgcctgcgg ttcgcacagc ggcgaatccg tccacgaagc gacggtggag gcgatgctgc
     6961 gtaaaatcgg gctggacggc ggcgcgctca agtgcggcgt gcacgagccg ttcgacaagg
     7021 aagttgcgct tgagttgcgt gcgcgcgggg agcagccgcg cgtcttgcag aacaactgct
     7081 ccggcaagca cacaggcatg cttgccctcg cgctccacct gggcggcgcg accgaaactt
     7141 acgaccagcc cgcgaacccc gcccaactcg ccatcgcacg caccgtcgca gagttttccg
     7201 gcgtcccgct tgaagagatc gcggtcggca cggacggctg cggcgtgccg gtctttggca
     7261 tcaccgtgcg cgcgatggcg ctcgcttatg cgcggctcgt cgcgccgccc gccgcgttcg
     7321 acgcaaagac gcgcgacgcc tgcgtgcgca tcgtcgtagc gatgcgtgcg cacccggaac
     7381 tcgtcggcgg cacgcgccag cggctcgaca cggagttgat gcgcgccgcg cagggcgtca
     7441 tctcgaaggt cggcgcggaa ggcgtctaca cggtcggcgt cgagccttcg gcgcgctggc
     7501 cgcgcgggtt ggggcttgcg ctcaagatcg aggacggcga agacaggcgc gcgcgtccca
     7561 ccgtcgtcat cgaatcgcta cgccaactcg gcgtattgga cgacgccacg ttcggagagc
     7621 ttgcgcccta tgcgaaattc gacgtgcgca atcatcgcgg cgacctggtg ggcgaagttc
     7681 gcgcttcttt tgagttggag cagtaaaaaa atcaataagg gcaaaaattt gcgggggctg
     7741 gaagtggcgt taaataggcc gtattgtgaa ttttgcgaat taaaataatt cagtggtaca
     7801 cggggtaagt ataaaaatcg tgcagatttc gggtggggct gaaagcggta agattgcagg
     7861 ccggatgggg cggaaatctt ccgaccccca tttttattga cgaaaattcg gatttcaaag
     7921 attgggcgac gacgggagaa gttgtggcat ggtcgttgcc aagagtagaa acagttaata
     7981 ttgataatga gtagatagtg ttatcgggtt ggctcggaga cggcagtgag tgacagcttt
     8041 gggggaggct ttctctcact ttatgtctgt agcggttcta tgggggaacg gaaacgttac
     8101 gggcaagcgg tcaaagagct ttagttgctg ggttgctggt caccgtctgt agtgctttgg
     8161 gggaacgcta caggcagtcc agcaacccgc aatctcgtca agggtaacgc tgcgaagtgc
     8221 tttaaagtaa aaaagcgcgt tgagtgttgg gggacactcg gcgcgctttt ttacgtttac
     8281 cactctttca actctcgctc aaacgcccgc gctcaaactt cttctacacg cttgcgccga
     8341 acttctcgcc tctatttatt tatctccttg gccgagcagg cgcgccgcgt tggtctgctg
     8401 gctcatttcg tctagattga ggcgcagcat gccgcccgcg tcggagaact gttcggtgag
     8461 tgcggcctgt gcgcggcggc gcgcgaaaat ccacccgccg aagatgccgc caatgctgag
     8521 acagatgacg atggcaagtc ccgtcgcctt caccgtgttg acgatgacgc tcgttgacat
     8581 gttgagccac gcgcgattcg cgcgttcggt ggcatagggg ttctcgccga gccagcgcac
     8641 ttcctgttca tatttcacct tgtcaatcaa ccctgccgcc gcgctctcgt ccggcgcacc
     8701 aaagacgaac accgcgtaat tcccgacgcg tcgataagcg gaaggcacgg gctgtcctgc
     8761 gccgcgcagt tcgctgaggc gcgcattgat ggcggcattg gcatcgaagg caaactgcgc
     8821 cgtcgcgtat tcgacaatga cgagccgcgc gccgccttcg taagtcgccg ttactgcttc
     8881 cgtcccgccg tcgaatgaga tggcatcgag cacgggctgg ttcggaacaa cctctctgag
     8941 cgcgggctgc gtgacgacgt agaccgcacg ctcctgcgcc gtctcccaat cgggcaggtg
     9001 cttgacgaga acgggaatct cccccgaacc gccttccaaa gtctccgcga aactacgtgc
     9061 gaaggtaacg agactcgcgt agttatcgtt tgcagaccct ccgtacacgc tggcgacggc
     9121 tgcgcctttg aagaatagaa cgcgcccgtc cagagagatc gccgccgtgc ctacgccgtt
     9181 caattgtgtg acgggcgaag agtgtgcgac aggcgaagag ggcgcagatg cttgcgccaa
     9241 cctgatgttg gttagcagcg catacgcttc ggaatcggac tgcgtcttga acagttggat
     9301 actaaattcg ctgccatcgg cggagacata agagcgcgtc gcgctgcgga cggcggcaaa
     9361 attttcctgt ttaagcggat cgaagaaagc gtgccgggga agacccgccg cgccgcgcgc
     9421 agtcgcctcg ccgatcttgt tcggcagtaa tttcgcggcg tctgcaaaag tagggcttgc
     9481 gtagacggag gcgaaaagta gagaaaaaag agagccgacg agaatcaatc tgttgataaa
     9541 tgggacacgc atggaaaatc ctttaacccc gaaaacggct aaaaatatga ctggcgacaa
     9601 tacgaccggc gacaagtatt tggatgccgg acggggaacg ctcgttgcgc tgagagtaat
     9661 agtaaccgcc ggggtctgtt tagttccaac tacttgttga ttccgttgac ggtttagttc
     9721 cagcggcgca gttcgcgcag caggagttcg gcgtggcggc gcgggtctgg gatgttgtcc
     9781 aagagcgtct ggccgccgac ttcgttggca ttttcgatga cgagatcgcc gaagccgaag
     9841 atgcgctgcg ggatggtggt ggagacggtt acgtcctgaa tgttgcgcag gggaatgttg
     9901 cgcgtcttgc gcgcgaggaa tccctgatcg atctcgatct tggagtcggt cagcgtgtac
     9961 ttgatggtgt tgcgtctgag gtgcttgtaa gcggggatca ggaggagcgg cagcgtgatg
    10021 agcacggcga tgggcgcgga gatgtactgc cccgcgaaca tcgcgagcag gacgctcagg
    10081 ccgacggcgg cgacggcggc gaggatgtag ccgatgccaa tgaagagaaa cgtcgggcgg
    10141 acgctgaaga tcacgcgctc ggcatccgcc tcgtcgcgcg tcgtgtgtgt ggcgggcgtt
    10201 cggacggcgt gttcgcccaa gggctgcgcc ggttgcctgc tgacggcggt tatcccgcta
    10261 tcgaaggagg tgcggacgcc cgccgtcgct tcgcccgtcc cgccgcgtga ggcgacgcgc
    10321 gtggcttccg tatcggcggg cgcgccgcac gatccgcaga aacggacgtt cgccggaata
    10381 tccgcgccgc agttcataca gtacatagtt ttcactcctt caatcggtgc tgccgaccgt
    10441 gcgcacgcag gtcaacagct cttccgggtc ataaggcact gcgacgaaag aactcacgcc
    10501 caactcagac gcttccgcgc gcacgcgttc gcgctccgtc gaactggccg gaagcaggag
    10561 cacgatgggc agcggcagtc gcccgtgatg ctggcgttgc aggcggcgca aggcttccag
    10621 cccgtcgatc ccgtagaggg caacgtcgat cacgagcaga tctggacgtt cgatgcgcag
    10681 caaattgatg gcttgcaggc cgtcgaacgc ggcgcgcact tcgtagcccg cgctgcgcag
    10741 caacagattg atctgcgcca tgcgcgccgg gtcggagacg gtgagcagga gacggcgcgg
    10801 catgatcgcg ccgcgcgtgc ggcgttcgcg ttcgcgcgcg gcggcatcgg cggcggcctg
    10861 tgccagcgtc ccctgcccga cgccaccgcc gcccgccccg cccgccggca tcatagaagt
    10921 ttccatgtcc atctgcttcg actcgcgtac ggcgcgcgac ggcgagagtt ctccggcggc
    10981 gcgtgcctgt cgccctgcgg tcggcgtctc cggcgcgacg ggcggttttg cgtcgcgcga
    11041 aacgagcggc ggcagcgtgg tcgaggcgtc gttcgccggg ggcttacttt ggggacgcgc
    11101 gtttggagag gtttcttcct cgtcttcccc ggcgtgcgcc gggcgcacct gttggagcgc
    11161 ggcttcggcg cgttcgatca aactttcgat ctccgtgccg tcggacggaa acgaggcgag
    11221 gccgacttcg agcgaaccgt ctgcgcgctc ctgcgtcgct tgaaattctt cgcttaaacg
    11281 cgcgatctct ttttgcgccg cgccgcgtgt cttttgcggc aagtaggcga gcaacaaagt
    11341 cgggccgtaa aaggcgaccg aatcgacgcg ccgcaggttg agcctgaaaa acttgtgagc
    11401 ctccgcgaaa gcacgcgcct gctgttgcgg catcgttgcg ccagcctcag tttccagaga
    11461 cgcttgagtt ggcgcggagg cgataagcgc gagcgtgccg cccgcgcccg cgtcgatgtc
    11521 ggcgcggacg gcttcgagca gttgcaga
//
LOCUS       DASWNS010000087         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_29123, whole genome shotgun sequence.
ACCESSION   DASWNS010000087 DASWNS010000000
VERSION     DASWNS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29123"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1629
                     /locus_tag="VGB76_03015"
     CDS             <1..>1629
                     /locus_tag="VGB76_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097903.1"
                     /translation="RTARAFIRAAVCLTLISQGLFIETTRRAAGQSGEEFISSVAVSP
                     SSVAVVNFADQAAAEENGGGSPSAPTVIPDSNSPSDPETGNTGAPANDDLSMRMPDEP
                     AEEEPPAQLSGEITPLTISDFQAQPDGGVIPPDTHGAVGLDKVMSTLNTNYQVQHKAT
                     GMVLSSLTIGGFWSPTGAINPYDPKTLYDPYNQRWILTAVSDRQTAASSILVAVSQTS
                     DPNGLYHLARFDADAGNSLHADFPSIGFNKNWITISVNMNTNSNPAFVESRVLAIDYP
                     SARGGLFNSPRYFTGITQGRGGFNIQPAVTYSATEPNLYLAAHGSAASATYSYFVMTG
                     TPALPGLRAAVTKNNLLGAWTQPGGDILPQAPEPNGTGTRKIDSRDSRILNMVYRNGS
                     VYFCQTVGLPAGRTPTTIERTAAQWVQVTASGDFVQGGRVEDPTATPTNGGKWYAYPS
                     LSVNRENSILMGFSQFASDEFASAAYTFRAVADPAGTMREPVVYKDGEGYYEKTLGGA
                     RNRWGNYSNTQVDPANDRDFWTIQEYAMTPSGTG"
BASE COUNT          325 a          584 c          463 g          257 t
ORIGIN      
        1 accgcacggc cagagcattc atcagagccg ccgtctgcct gacgttgatc tcgcaaggct
       61 tattcatcga aaccacgcgc cgcgcggcgg ggcagagcgg cgaggagttt atttccagcg
      121 tggccgtcag cccgtcgagc gtcgccgtcg tcaacttcgc ggatcaagcg gcggcagaag
      181 agaacggcgg cggcagccct tccgcgccca cagtcatccc cgattcaaac tcgccctccg
      241 acccggagac gggaaatacg ggcgcgcccg caaacgacga tttatctatg cggatgccgg
      301 acgaaccggc tgaagaagag cctcccgcgc agttgtcggg ggagatcact ccgctcacga
      361 tcagcgattt tcaggcgcag ccggacggcg gcgtcatccc gcccgacacg cacggcgcgg
      421 tcggcctcga caaggtcatg tcaacgctca acacgaatta tcaggtgcag cacaaagcga
      481 cgggcatggt tctcagcagc ctgacgattg gcgggttctg gtcgccgacg ggcgcaatca
      541 acccttacga cccgaagacg ctctacgatc cgtataacca gcgttggatt ctcaccgccg
      601 tctcggacag gcagacggct gcctcttcga tcctcgtcgc cgtctcgcaa acctccgacc
      661 cgaacggcct ctaccatctc gcgcgcttcg acgccgacgc gggcaactcg ctccacgcag
      721 attttccgag catcggcttc aacaagaact ggatcaccat ctcggttaac atgaacacga
      781 acagcaatcc cgcgttcgtc gaaagccgcg tgctcgcgat tgattacccg tcggcgcggg
      841 gcggcctctt caactccccc cgctacttca caggcatcac gcaggggcgc ggcggcttca
      901 acatacagcc cgccgtcact tattcagcca cggaaccgaa cctgtatctc gccgcgcacg
      961 gcagcgccgc gtcggccacc tattcctatt tcgtgatgac gggcacgccc gccctccccg
     1021 gcctgcgcgc cgccgtcaca aagaacaacc ttctcggcgc gtggacccaa ccgggcggcg
     1081 acattctccc gcaagcgccg gagccgaacg gcacgggcac gcgcaagatc gacagccgcg
     1141 acagccgcat cctcaacatg gtctatcgca acggctcggt ctatttctgc cagaccgtcg
     1201 gcctgcccgc cgggcgcacc cccacgacca tcgaacgcac cgccgcgcag tgggtgcagg
     1261 tcaccgccag cggcgacttc gtgcagggcg ggcgcgtcga agacccgacc gccacgccta
     1321 ccaacggcgg caagtggtac gcctaccctt cgctctccgt aaaccgcgag aacagcatcc
     1381 tgatgggctt ctcgcagttc gcttccgacg agttcgcttc cgccgcctac accttccgcg
     1441 ccgtcgccga cccggcgggc acgatgcgcg aacccgtcgt ctataaagat ggagaaggct
     1501 actacgaaaa aactttgggc ggcgcgcgca accgttgggg caactacagc aacacgcagg
     1561 ttgaccctgc caacgaccgc gacttctgga cgatacagga gtatgcgatg accccttcgg
     1621 ggacgggca
//
LOCUS       DASWNS010000088         9138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_288998, whole genome shotgun sequence.
ACCESSION   DASWNS010000088 DASWNS010000000
VERSION     DASWNS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9138
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288998"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VGB76_03020"
     CDS             complement(<1..756)
                     /locus_tag="VGB76_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0097904.1"
                     /translation="MLLETDFIVIGSGIAGLRAALEISGRGARVVVLTKERASESNTE
                     YAQGGVAVALSEDDETTLHEDDTLAAGAGLCDERAVRVLVQDGRRYMRELMEWGAEFD
                     REGGELLFTQEAAHSRRRILHAGGDATGHEIVRALLARARADKRIEFLAHAATEELIL
                     EDGRCRGVRYLDPLVRASRSLHARAVVLASGGAGQLYLHTTNPQVATGDGMAMAYAAG
                     AEVADMEFVQFHPTALNVEAAPRFLLSEAMRGEG"
     gene            968..2677
                     /locus_tag="VGB76_03025"
     CDS             968..2677
                     /locus_tag="VGB76_03025"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HEY0097905.1"
                     /translation="MRKIRLGNSLPVCAAGLLFGALPLWRGVPVRGDTVFHAMWYTHF
                     SAQLLAGDLYPRWLINLNGGLGSPVFFFYAPLPYYITTLFAFVMPGGIYGTLHLGASA
                     ALAIMASGLTAYLWLKESADAKAASVAAVLYMLMPYHLAVDVYTRDAFGEVWAFVWMP
                     LLLYFVRRTGGGSKFAPHGLALAYAALITTHLPSTLIFSLVPPCYAFFAHEPGRRGRA
                     LLYTAGGMLLGTGLAAVYLLPAMLNQAFISLGDMLPDDYDQRWLRLTDFNLQLIEGRI
                     TIAFLLTAGVIACAYLMARGGAQANDAAFRRERAFWMSVSAACLLLMTAPGDFVWRIV
                     KPLRSIQFPWRFNAVLCVAAAALIAAGVRALLRNRPPGALRIAASVAGCALVLGWVVF
                     TVQVARKEFPSLRRGSPAGMDAAYLKRLEQGRDAPEYRPANAASTQTEEFAKLLSRIC
                     RDGAQLAKACVVEGAGAVTFERWQPREIALEVETPQGLKLNVNQFYYPGWTAYVDGRA
                     HPLAPSQPDGLLQLTLPPGAHKLKLRLEKTTPERAGEFISAASVILWLLSLVAAKRSR
                     SEW"
     gene            complement(2708..3163)
                     /locus_tag="VGB76_03030"
     CDS             complement(2708..3163)
                     /locus_tag="VGB76_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY0097906.1"
                     /translation="MRARTEATRTRDFAATEGIEARKSKIDGTGCFATVPFRKRRKIA
                     ELTGERVSRVKAARRMRGKQRLHICAIDSYWGIDSSVGGNAAQFINHSCQPNSFVRIM
                     HGHIIFFALRDIAPGEEITLDYVESYHSNDKRCDCGAPNCRGTINLIKK"
     gene            complement(3160..3519)
                     /locus_tag="VGB76_03035"
     CDS             complement(3160..3519)
                     /locus_tag="VGB76_03035"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY0097907.1"
                     /translation="MMDIESLRRFCLSLPHATEGVQWDNDLLFRVGGKMFAVASLDVT
                     PTRVSFKCTPEKFAELVEQEEITPAAYMARIHWVTLHRLDALNERETKELIRESYGMV
                     WEKLTKKLRAELEAGKH"
     gene            complement(3621..4292)
                     /locus_tag="VGB76_03040"
     CDS             complement(3621..4292)
                     /locus_tag="VGB76_03040"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEY0097908.1"
                     /translation="MSEELILSAEELRVVGALIEKQVTTPEYYPLTLNALRQACNQLS
                     NRDPVVSFDERTVAWALESLRDRKLVRVVSTADGRVPKYWHVLDEALGLKPPEMAVMC
                     VLMLRGAQTIGEIRTRTERLYPFSALSFVETTLEDLMTREVPLVLKLPRLAGQKESRY
                     AHLLGGEVEVPAESVAAPRVEAATREVRGELERVAHLENELKTVRAELAELREQFAEF
                     KKQFE"
     gene            complement(4309..4695)
                     /locus_tag="VGB76_03045"
     CDS             complement(4309..4695)
                     /locus_tag="VGB76_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0097909.1"
                     /translation="MVKRLLIDTDVLIDYLRGQAEAVSYLESLTEPLLISAITVAELF
                     AGVREGTERVALEQFVSAFNVIPIDDAIAANGGLIRRDYRKSHGVGLADAIIASTAEF
                     TKADLVTLNKKHFPMLSNVVTPYHKS"
     gene            complement(4714..4947)
                     /locus_tag="VGB76_03050"
     CDS             complement(4714..4947)
                     /locus_tag="VGB76_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY0097910.1"
                     /translation="MIETEINLTAQERDALKEIAERTGKSQAELVREAVERLISEFQR
                     EDDRNLLRQAQGIWKDHVDLPAPEELRREWDRS"
     gene            complement(5027..5863)
                     /locus_tag="VGB76_03055"
     CDS             complement(5027..5863)
                     /locus_tag="VGB76_03055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0097911.1"
                     /translation="MNEQDGQPFIFADANLSRRLERAEGHTNREFVETRARMFPASGA
                     CWREVAGAYLLFDGAESLLTQTFGLGLFQSVTGAEMDEIESFFSERGAPVLHEVSPLA
                     DPALLPLLNERGYQPIELTSVMFRPIKSAGDKPHAPPNDKINVRIVGEDEHELWAQTA
                     AHGWSEFTEFADQILELSRVSAKRASAASFIAEIEGRPIATGGMGICDSVALLAGAST
                     IPEGRRQGAQTALLDSRLHFAAAQGCDIAMMCAQPGSTSQRNAERNGFRIAYTRIKWR
                     LD"
     gene            6025..7128
                     /gene="rlmN"
                     /locus_tag="VGB76_03060"
     CDS             6025..7128
                     /gene="rlmN"
                     /locus_tag="VGB76_03060"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY0097912.1"
                     /translation="MNHQAVHLTGLDAAELSAFVEESGEPAYRGRQLFGALHRRRLRS
                     FEEMTDLPKSFRATLAGRATASTLTVESRYISEDGTRRYLMKTHDNLPVETVFIPEER
                     RDTICFSSQSGCPLQCDFCLTAKLGLLRTLTAGEIVEQIVIALNDAYGAGVKTPRGTN
                     LVAMGAGEPFLAFEPLMKALRIMADPAGLHIVPNRVTVSTAGIVPRIRELARIPSRPH
                     LAISLAAPTDEQRNVLMPINRKWPLAELLDACKEFELSLKPGERFTFEYVMLDGVNDS
                     DEHARQLANLLNRHQLRAKVNLIPHNPAEPLPYRPTPLEQVERFKRILEAKGVHAYVR
                     RPRGRDIYAACGQLAARRPANNLNPQLLHQLTG"
     gene            7166..7768
                     /locus_tag="VGB76_03065"
     CDS             7166..7768
                     /locus_tag="VGB76_03065"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY0097913.1"
                     /translation="MTRKIFITRTILLFIAATLIVAAPPAPRAASAAVENAAVATNLS
                     APAAVQGNIGVNGFFSADKAQRGRSIQAAVVLDIPRGFHVNANKAAKFSIPTVVKIDA
                     PRGLRVGAVVYPRAVVRRLSFSKEALSLYEGRAVMRFNLSVPANFQTGLTELRAKVTY
                     QSCNDEVCFPPTTREVKLGINVVGTNEPVKRINAQYFGRR"
     gene            complement(7858..8718)
                     /gene="pgeF"
                     /locus_tag="VGB76_03070"
     CDS             complement(7858..8718)
                     /gene="pgeF"
                     /locus_tag="VGB76_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY0097914.1"
                     /translation="MPIEMIKETSEPLARAGFNWREQGGVRALVCTQLEAEGFTNGFS
                     TRAGGCSPMPHDALNLAGFNEDDAANIYENRRRFLSLFRGQWRLATCWQVHGADVRRV
                     VPRDATHALDTNEERCDALTTDAAHVLLGVQTADCVPIILGDARTGACAAVHAGWRGT
                     AASIVVAALERMREDYGTRSADVRACIGPAANVCCYEVGREVLDTFRARFAYADELLA
                     PTREGHARIDLQRANRQQLAGAGVAPDRIHTAPFCTICRNDLFFSYRREKKVHGRTGR
                     LLAVIGKSDE"
     gene            8699..9088
                     /locus_tag="VGB76_03075"
     CDS             8699..9088
                     /locus_tag="VGB76_03075"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY0097915.1"
                     /translation="MIISIGIDIIEVARVRRVLRRTPRFAERVFTPGERDYCERRGVV
                     APQHYAARFAAKEAALKALGTGWSGGLAWQDIEVVSNAAGAPSLSFYNLARELLERSG
                     ATTAHLSISHTTEHAIAQVVLERRDVL"
BASE COUNT         1650 a         3063 c         2621 g         1804 t
ORIGIN      
        1 gccctcgcca cgcatcgctt cggagagcag aaaacgcggc gcagcctcta cgttcaaagc
       61 cgtcgggtga aactgcacaa actccatgtc agccacttcc gcgcccgccg cgtaggccat
      121 cgccatgccg tcgcccgtcg cgacctgcgg gttggtcgtg tgcagataga gttgacccgc
      181 gccgcccgac gcgagcacca ccgcgcgcgc gtgcaggctc cgcgacgcgc gcaccagcgg
      241 atcgagataa cgcacgccgc gacaacgccc gtcttccaag atcaactctt cggtcgccgc
      301 gtgcgcgaga aactctatgc gcttgtcggc acgcgcacgc gcgagcagag cgcggacgat
      361 ctcgtgcccg gtcgcgtcgc ctccggcgtg gaggatgcgg cggcgcgagt gcgcggcctc
      421 ctgcgtgaag agcagttcgc cgccttcccg gtcaaactcc gctccccatt ccatcaactc
      481 gcgcatgtag cggcgaccgt cttgcacgag cacgcgcacg gcgcgttcgt cgcatagccc
      541 tgcgcccgcc gccagtgtgt cgtcctcgtg gagcgtcgtc tcgtcgtctt ccgagagcgc
      601 gaccgccacg ccgccctgcg catactccgt gttcgattcg ctcgcgcgct ccttcgtgag
      661 cacgacgacg cgtgcgcccc ggcctgagat ttcgagcgcg gcgcgcaagc ccgcgatacc
      721 gctgccgata acgatgaagt ctgtttcaag aagcatcttg gtgttggaaa gttctcagcg
      781 tgacatacta acaacccgcc gcgcgcgcgg gcaacgtcca cggcgcttcc gctgcgcggc
      841 tgcgcgtcct gcgtccggcc tccttcttga accggcgcgc tcacgtaaga taaagtggcg
      901 gagaagcgtc catcacttga agtcgcacgc ggctgcgcgc cgcagttcac gtctggccga
      961 cgcccggatg agaaagatca gactcggaaa ttctctgccc gtctgcgccg ccggcctcct
     1021 gttcggcgcg ctgcctctgt ggcgaggcgt gcctgtccgg ggcgacacgg tgttccacgc
     1081 gatgtggtac acgcacttct ccgcgcaact tctggcgggc gacctctacc cgcgctggct
     1141 catcaatctc aacggcgggc tgggcagccc ggtctttttc ttctacgccc cgctgcccta
     1201 ttacatcacc acgctcttcg cgttcgtgat gcccggcggc atctacggca cgctgcatct
     1261 cggcgcatcc gccgcgctgg cgatcatggc ctccggcctc acggcctacc tctggctcaa
     1321 agagagcgcg gacgcgaaag cggccagcgt cgccgccgtc ctctacatgc tcatgcccta
     1381 tcatctcgcc gtcgatgttt atacgcgcga cgcgttcgga gaggtgtggg ctttcgtctg
     1441 gatgccgttg ctgctctact tcgtacggcg caccggcggc ggctcgaagt ttgccccaca
     1501 cggactcgcg ctcgcctacg ccgcgcttat caccacacac ctgccgagca ctttgatctt
     1561 ttccctcgtc ccgccctgtt atgccttctt cgcgcacgag ccggggcgca gaggccgcgc
     1621 gctgctctac acggcgggcg gcatgctgct cggcacgggg ctggctgccg tctacctgct
     1681 cccggccatg ctcaatcagg cattcatctc tctcggcgac atgctgccgg atgactacga
     1741 ccaaaggtgg cttcgactaa ccgacttcaa cctccaactc atcgagggac gaatcacaat
     1801 agcgttcctg ttaaccgccg gagtgatcgc ctgcgcctac ctcatggcgc gcggcggcgc
     1861 gcaggcgaac gacgctgcgt tcaggcgcga gcgcgcattc tggatgagcg tgtcggcggc
     1921 ctgtctcctg ttgatgaccg cgccgggcga cttcgtgtgg cggatcgtta agccgctcag
     1981 gagcatccag ttcccctggc gtttcaacgc ggtgctttgc gtcgccgccg ccgcgctcat
     2041 cgcagcgggc gttcgcgctc tccttcgcaa cagacctccc ggcgcgctca ggatcgcggc
     2101 gtcagtcgcc gggtgcgcgc tcgttctggg ttgggtggtc ttcacagtcc aggtggcgcg
     2161 gaaggagttt ccatcgctca ggcgcggctc gcctgcgggg atggacgcgg cctatctcaa
     2221 gcggcttgag cagggacgcg acgcgccgga gtatcgcccg gccaacgccg cttcgacgca
     2281 gaccgaagag ttcgcgaagc tgttgtcgag aatctgccgc gacggcgcgc agttggcgaa
     2341 ggcgtgcgtc gtcgaagggg cgggcgcggt cacgtttgag cgttggcaac cgagagagat
     2401 cgcgctcgaa gttgagacgc cgcagggact caagctcaac gtcaaccagt tttactatcc
     2461 cggctggacg gcctacgttg acgggcgtgc gcacccgctc gcgccctcgc agcccgacgg
     2521 acttctccaa ctcacacttc ctcccggcgc gcataagttg aaattacgct tggagaagac
     2581 gacgccggaa agagccggag agttcatcag cgcggcctcc gtcatcctgt ggctgctctc
     2641 gctcgtggcc gcgaagcgaa gccggagcga atggtgaaag ctgcctctgt gagctaaacg
     2701 gcgggagcta ttttttgatg aggttgatcg tcccgcgaca gttcggcgcg ccgcaatcgc
     2761 aacgcttgtc gttcgagtga taggattcga cgtagtcgag cgtgatctct tcgccgggtg
     2821 cgatgtcgcg cagcgcgaag aagatgatat ggccgtgcat gatgcgcacg aagctgttcg
     2881 gctggcatga atgattgatg aactgcgcgg cgttgccgcc cacgctgctg tcaatgcccc
     2941 agtaagaatc tatggcgcag atgtgcaggc gttgcttgcc gcgcatccgc cgcgccgcct
     3001 tcacgcgcga cacgcgctcg cccgtcaact cagcgatctt ccgtctttta cgaaacggca
     3061 cggtggcgaa acaacccgtc ccgtcaatct tcgacttgcg cgcctcgatg ccctccgtcg
     3121 cggcaaagtc tctcgttcgc gtcgcttcag ttctcgctct caatgtttac cggcctccaa
     3181 ctccgcacgc agtttcttcg tcaacttctc ccacaccatc ccgtaagact cgcgtatcaa
     3241 ctctttcgtc tcccgctcgt tcagcgcgtc gagccggtgc agcgtcaccc aatggatgcg
     3301 cgccatgtag gcggcgggcg tgatctcctc ctgctcgacg agttcggcaa acttctccgg
     3361 cgtgcatttg aaggacacgc gcgtcggcgt cacatccaga ctcgccaccg cgaacatctt
     3421 cccgccgacg cgaaacaaca gatcgttatc ccactgcacg ccctccgtcg catgcggcag
     3481 cgacaggcag aaacggcgca agctctcaat atccatcatc tcaacctctc ttctgctgtg
     3541 acgctctcac gttctcttaa gcaactctcc gcacctgtga agcgaattct ctttaacatt
     3601 cgattcatga atcagacgcc ctactcaaac tgcttcttaa actccgcaaa ctgctcacgc
     3661 aactccgcca actccgcacg caccgtcttc aactcgttct ccaaatgcgc tacccgctcc
     3721 aactctccac gcacttccct cgtcgccgct tcgacgcggg gcgcggccac gctctccgcc
     3781 ggaacctcca cctccccgcc gagcaggtgc gcgtaacgcg actccttctg tcccgccagc
     3841 cgtggcaact tcaagacgag cggcacctcg cgcgtcatca aatcttccag cgtcgtttcg
     3901 acaaacgaca aggccgagaa cggatagaga cgctccgtgc gcgtgcgtat ctctcctatc
     3961 gtctgcgccc cgcgcagcat caacacacac atcaccgcca tctccggcgg ctttaagccc
     4021 aacgcctcgt ccaacacatg ccagtacttc ggcacgcgcc cgtccgctgt gctcaccacc
     4081 ctcacgagtt tccggtcgcg caaactctcc aaagcccacg ccaccgtccg ctcgtcaaac
     4141 gacaccaccg ggtcgcggtt cgacaactga ttacacgcct gccgcagcgc gttcaacgtc
     4201 aaagggtaat actccggcgt cgtcacctgc ttctcgatga gcgcgcccac cacgcgcaac
     4261 tcttccgcac tcaagatcaa ttcttcgctc aaggctttat ccttatgatt aacttttatg
     4321 gtagggagta accacgttcg acaacatcgg gaaatgcttc ttgtttaaag tcactagatc
     4381 ggcttttgta aactcagcgg tagacgcgat tatggcgtct gcaagtccga ctccgtgact
     4441 tttcctatag tcgcgcctga tgagtccacc attggcagca atagcatcat cgatagggat
     4501 gacgttgaag gctgagacga actgctccaa agctactcgc tctgtaccct cgcgcacacc
     4561 tgcgaaaagt tcggccaccg taatggcaga aatcagcaac ggctctgtga gactctctag
     4621 atacgaaacg gcctccgcct gcccacgtaa gtagtctata agaacatcgg tatctatcag
     4681 caaccgttta accatgtgag ccgctcatct cccttaactt cgatcccatt cccgtctgag
     4741 ttcttccggt gcgggtaaat caacgtggtc tttccagatg ccctgcgctt ggcgcaggag
     4801 gttgcgatca tcttcccttt gaaactcact gatcagtcgc tcgaccgctt cacgaaccaa
     4861 ctcggcttgt gacttgcccg tccgttcggc aatctccttg agcgcgtccc gctcctgtgc
     4921 agtcaaattt atctctgtct caatcatttg caacacacct ttcacgagca acgaacctga
     4981 acatatgtta tcagaactca agactaactc tctgctcgat gccttatcag tccaatcgcc
     5041 acttgatgcg cgtgtaggcg atgcggaatc cgttgcgctc ggcgttgcgc tgcgaagtgc
     5101 tgccgggttg ggcgcacatc atggcgatgt cgcagccctg cgcggcagca aagtgcaggc
     5161 ggctgtcgag caaagccgtc tgcgcgccct gcctgcgccc ttcggggatc gtgctcgccc
     5221 ctgccagcag ggctacgctg tcgcagatgc ccatgccgcc cgtggcgata ggccgtccct
     5281 cgatctcggc gatgaaagag gcggcggacg ccctcttcgc gctgacgcgt gagagttcga
     5341 gtatctggtc tgcgaattcc gtgaactcgc tccacccgtg cgccgccgtc tgcgcccata
     5401 gctcatgctc gtcttctccg acgatccgca cgttgatctt atcgttcggc ggcgcgtgcg
     5461 gtttgtcacc cgccgacttg atcgggcgga acataacgct cgtaagttcg atgggctgat
     5521 agccgcgctc gttcagcaga gggagcagcg cagggtcggc cagcgggctg acttcatgca
     5581 ggacgggcgc gccgcgctca ctaaagaaac tttcgatctc atccatctcc gcgcccgtca
     5641 cggactggaa gaggccgagg ccgaacgtct gcgtcaagag tgactccgcc ccgtcgaaca
     5701 acaggtatgc gcccgccact tcgcgccagc acgcgccgga tgcgggaaac attcgcgccc
     5761 ttgtttccac gaactccctg ttcgtatgcc cttccgccct ctccagtcgc cgcgacaggt
     5821 tggcatcggc gaagatgaac ggttggccgt cctgttcatt catagttttt tctgtccaat
     5881 gctcacattt gaactctgga attatactcc agggcgcagg atagcacgcc gctctcccgt
     5941 ccggcgcgcg tttgttgcgc gcgaaattta tgccgcccgc cctgctgtgt gttgggcgtc
     6001 gcgcgatgtt aaaatagcgt tgcaatgaat catcaagcag tacatctcac agggttggac
     6061 gcggcggaat tgtctgcgtt tgtcgaagag tcgggcgaac ccgcgtatcg cgggcggcag
     6121 ttgttcggcg cgctgcaccg caggcgtctg cgttcgttcg aagagatgac ggacttgccg
     6181 aagagttttc gcgcgacgct tgccggacgc gcgaccgctt cgactctgac cgtcgaatcg
     6241 cgttacatct ccgaggacgg cacgcgccgt tacttgatga agacgcacga caatcttccc
     6301 gtcgaaaccg tcttcattcc cgaagaacga cgcgacacga tctgtttctc gtcgcaatcg
     6361 ggctgccccc tgcaatgcga tttttgtttg acggcgaaac tcggactcct gcgcacgctg
     6421 acggcgggcg agatcgtcga gcagatcgtg atcgctctga acgacgccta cggcgcgggc
     6481 gtcaagactc cgcgcgggac gaacctcgtg gcgatgggcg cgggcgaacc cttcctcgcc
     6541 ttcgagcctt tgatgaaagc tctgcgcatc atggccgacc ccgccggtct gcatatcgtg
     6601 ccgaaccgcg tcaccgtctc gaccgcaggc atcgtgcccc gcatacgcga actcgcgcgc
     6661 atcccttcgc gcccgcatct cgccatctca ctcgccgcgc ccacagacga acagcgcaac
     6721 gtcctgatgc cgatcaatcg caaatggccg ctcgcggagt tgctcgacgc gtgcaaggag
     6781 ttcgagttgt ctctgaagcc gggcgagcgt ttcacgttcg agtacgtcat gctcgacggc
     6841 gtgaacgatt cagacgagca cgcgcgccaa ctggcgaacc tgctcaaccg tcaccaactg
     6901 cgcgcaaagg tcaacctgat tccgcacaac cccgccgagc ctctgcccta tcgcccgacg
     6961 ccgttggagc aggtggagcg attcaagcgc atcctcgaag cgaagggcgt gcatgcctac
     7021 gtgcgccgcc cgcgcgggcg agacatctat gccgcctgcg gccaactcgc cgcgcgccgc
     7081 ccggcaaaca acctgaaccc gcaactgctg catcaactaa caggctgacc gctcaactca
     7141 aaaatcaagt ctatcgaaag ccgaaatgac ccgaaaaata tttatcacgc gcaccattct
     7201 gctcttcatc gccgccacgc tcatcgtcgc cgcgccgccc gccccgcgcg ccgcctccgc
     7261 tgccgttgag aatgctgctg tcgcaaccaa tctctctgcg cccgccgccg tgcagggcaa
     7321 catcggcgtc aacgggttct tctccgcaga caaagcgcaa cgcggtcgga gcattcaggc
     7381 cgccgtcgtg ttggacatcc cgcgcggctt tcacgtcaac gcgaacaagg ccgccaagtt
     7441 ttcgatcccg accgtggtga agattgacgc gccgagaggc ttgcgcgtgg gcgcggtggt
     7501 gtacccgcgc gccgtcgtgc gccgtctcag tttctcgaaa gaggctctgt cgctctacga
     7561 agggcgcgcc gtgatgcgct ttaacctctc cgtcccggcc aactttcaga cggggctgac
     7621 cgaactgcgc gcaaaggtga cctaccaaag ctgcaacgac gaagtctgct tcccgccaac
     7681 cacgcgtgaa gtcaaactcg gcatcaacgt cgtcggcaca aacgaacccg tcaaacgcat
     7741 caacgcgcaa tacttcggaa gaaggtaaga aagtggcgag gaaggaagtg atgagtgatg
     7801 agtaatgagt gatgagttaa aacagaagcc tcttcttcac tcattactca tcactcatca
     7861 ctcatcactt tttccgataa ctgcgaggag tcgtccggtg cgaccgtgga cttttttttc
     7921 gcgccggtag gagaagaaga ggtcgttgcg acagatggtg cagaagggcg cggtgtggat
     7981 gcgatcgggt gcgacgcccg cgcccgcgag ttgttggcgg ttggctcttt gcagatcaat
     8041 gcgcgcgtgc ccttcgcgcg tgggcgcgag aagttcgtcc gcgtaggcga agcgcgcgcg
     8101 gaaagtgtcg agcacttcgc gcccgacttc gtagcagcag acgttggcgg cggggccgat
     8161 gcaggcgcgc acgtccgccg agcgcgtgcc gtaatcttcg cgcatccgtt cgagcgcggc
     8221 gacgacgatt gacgccgccg tgccgcgcca tcccgcatgg acggcggcgc aggcgcccgt
     8281 tcgcgcgtcg cctaagatga tgggcacaca gtcggcggtc tggacgccga gcaggacgtg
     8341 tgcggcgtcg gtcgtcagcg cgtcgcagcg ttcttcgttg gtgtcgagcg cgtgtgtcgc
     8401 atcgcgcggg acgacgcgcc gcacgtctgc gccgtgaacc tgccagcagg tggcgagtcg
     8461 ccactggcct cgaaagagcg agaggaagcg gcgtcggttt tcgtagatgt tcgccgcgtc
     8521 gtcttcgttg aagcccgcga gattgagcgc gtcgtgcggc atggggctgc acccgcccgc
     8581 ccgcgtggag aagccgttcg tgaagccttc ggcttccaac tgcgtgcaga ccagggcgcg
     8641 cacgccgccc tgttcgcgcc aattgaatcc ggcgcgtgcg agcggttcag aggtttcctt
     8701 gatcatttca atcggcatag acatcatcga agtggcacgt gtgcgccggg tcttgcgccg
     8761 cacgccgcgt ttcgccgagc gcgtcttcac tcccggcgag cgcgactatt gcgagcggcg
     8821 gggcgtggtc gcgccgcaac attatgccgc gcgtttcgcc gcgaaagagg ccgcgctgaa
     8881 agcgctcggc acgggttgga gcggcggcct cgcttggcaa gacatcgagg tcgtgtcgaa
     8941 tgcggcgggc gcgccttcgc tcagtttcta caacctcgca cgcgaactac tcgaacgctc
     9001 cggcgcgacg acggcacatc tctccatatc tcacaccacc gaacacgcta tcgctcaggt
     9061 cgtcctcgaa cgccgcgacg ttctctaaac gacgacacgc gaattgtaac ccagacaccg
     9121 acaattaaaa gccgtgtg
//
LOCUS       DASWNS010000089         6147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_289039, whole genome shotgun sequence.
ACCESSION   DASWNS010000089 DASWNS010000000
VERSION     DASWNS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289039"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..446)
                     /locus_tag="VGB76_03080"
     CDS             complement(246..446)
                     /locus_tag="VGB76_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097916.1"
                     /translation="MVHVRFEGRSFDYAEREVRVEASMTDGEIKARLAQFLDASMDRF
                     EAYVVERTQLGDLIIRPEAVYG"
     gene            complement(596..805)
                     /locus_tag="VGB76_03085"
     CDS             complement(596..805)
                     /locus_tag="VGB76_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097917.1"
                     /translation="MRRQRESASGKGAASVVTRSGSPSAGAAAFKRGGAESLCAAASL
                     ESVLDKVRLAWQEGSTAFRNRQVPA"
     gene            complement(977..3742)
                     /locus_tag="VGB76_03090"
     CDS             complement(977..3742)
                     /locus_tag="VGB76_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0097918.1"
                     /translation="MRQQFSPEQDEGERAWTPYVVLAVTLLLTAFSIYYVVQKAEEKG
                     RQLFETAGGHLTYILLGGLLTGFVLFWLTRAQARARASAKRTAAKLRQSEERFRTLIE
                     QSPLSTQVFSPDGRTIQVNRAWEELWGVTLETLGDYNILEDPQLVAKGIMPYIQKGFA
                     GTPTSIPPILYDPEVTIPNVTVNEHPQRWVQAIIYPVRDAAGEIREVVLMHEDISERK
                     RVEEDLRYQLNLTSAITENTAEGLCLMDAEGRLTFMNPAAEAMLGWKEAELKGRVLHH
                     AVHYLKPDGTPFPLSECPLGDVLNSKATITNREDVWIRKDGTMIPIFCSCAPIMVDGV
                     LTGAVLAAHDITERKRAEERRAQRARQTTLRADVSVALAKSDSLQSVLRESAEALVSH
                     FDAAFARIWTFNPDTNFLELQASAGIYTRLDGTHARVPLGQFKIGLIAEERKPHRTND
                     VLNDPRVSDKEWARRTGMVAFAGYPLLVEDRLIGVMAIFSREHLTEETLDSLALVAET
                     IAQGIERKRLEEQLRQRAEQLAEANRLKDEFLATLSHELRTPLTSILGWAKLMRTEKF
                     DPTLSNRALEIIERSAVAQSQLIDDLLDVSRIITGKLRLVPQTLELAPIMAAAVDSLR
                     PTADARGVQLEMLLDTTAGQVSGDADRLQQVIWNLLSNAIKFTPKGGRVTIELRRSDS
                     QVEIVVSDTGQGIAPEFLPYIFDRFRQADGSITRAHGGLGLGLAIVRHLVELHGGTIR
                     AASYGANLGSTFRVRLPLVGVRHSTDPHGLADARHASGTSDGAFNAAARNGREGVLKG
                     LRVLLVEDDPDTRSLLVTTLEQSGAERVAACASAPEALDALKRLLPDVLISDIGMANV
                     DGYELIRQIRALPPEEGGRTPAAALTAYATPGDRLRALDAGYNLHIPKPIGPKELAAA
                     IARLAGR"
     gene            complement(4069..5322)
                     /locus_tag="VGB76_03095"
     CDS             complement(4069..5322)
                     /locus_tag="VGB76_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0097919.1"
                     /translation="MRSEQPRRKTRLLIADDEPEIRSILQECLCEHYDCRAVGSAEEV
                     LALLTTSNFDLIISDINMEGMSGLEMVPHVRQIAPETVIIMISGAQTVESAIEALRAG
                     AFDYVTKPFDLRHVEMAVERAREHRELLRSKRRYELFLEESIKLRTKELGEALASLED
                     AYRTTLKALAAALETRDTDTHGHSERVVSFSLRLGRELDLDEAQLRALEFGSLLHDIG
                     KIGIPDCILRKPAALTDEEWTTMRQHPALGGQILRGIKFLEGAARVVGQHHERWDGTG
                     YPSGLRGAQIDLNARIFAVADAFDAMVSTRVYRAGKPYEAAAEELKRHAGQQFDPDVV
                     AAFLRIPPAEWEELRSHSLRHRPELLAEAQTETHAHVAAASASSSSTGKAGTPLLPSL
                     APKAPRIVHHPIKIRTIQRRGVRHS"
BASE COUNT         1105 a         1901 c         1910 g         1231 t
ORIGIN      
        1 ggcagggacg caaaccgaaa ctcaaagaac aaaaaaaagc ggttgctccc tcatctctca
       61 atgtatgact tggtatgacg tgccccgcgc aagtccgaaa agcatttttc acatcggtgc
      121 tctgccactg agctacagga gcaaaaattt ttgcgctcct gacgggaatc gaacccgcga
      181 ccccccactt aaaggtgact gtgtgtaggc ttctcgcgtt ggggcgcggg gacgcgccac
      241 tcctcttacc cgtagacggc ctccgggcgg atgatcaggt cgccgagttg cgtgcgctcg
      301 acgacatacg cctcgaagcg atccatgctc gcgtcgagaa actgcgcgag acgcgccttg
      361 atctcgccat ccgtcatcga cgcctcgacg cgcacctcgc gctccgcgta atcaaaagaa
      421 cgaccttcaa aacgaacgtg taccattttt ttcgcacctc ctccgaaaca cgaatcttac
      481 cgatgacaaa aaagagcggg tcgctcgctt ccctccacgg aagccgaccc gctccttcaa
      541 atctcttaaa ctttgcgttt gagaatttcg ggagcgggag gccgctcccg caaaatcagg
      601 ccggcacttg acgattacgg aaagccgtcg agccttcctg ccacgccaat cgcaccttat
      661 cgagcacgct ttcgagcgac gccgccgcgc acaggctttc tgcgccgccg cgcttaaacg
      721 cagccgcgcc cgcagagggc gagccggagc gtgtcacgac agacgccgcc cctttgccgc
      781 tcgccgactc tctctgtctt cgcatcctca acatgctctc catcgtctct tctctttgcc
      841 gcgccgcgcg gggaaaatct cgaccgcgcg tgtgcgccgg taatatatag accgcgacgc
      901 ttctcctgtc aacacttttt tctgttaaca gcagatttat ttttgtcgct gccgggggaa
      961 cgactgcggg cttcgcttat cgtccggcga ggcgtgcgat ggcggcggcc agttctttcg
     1021 gcccgatggg tttggggatg tgcaggttgt agcccgcgtc gagcgcgcgc aggcggtcgc
     1081 cgggggtggc gtaggcggtg agcgcggcgg cgggcgtgcg tccgccctct tccgggggga
     1141 gcgcgcgaat ctgccgtatg agttcatagc cgtcaacgtt cgccatgccg atgtcggaga
     1201 tgagcacgtc gggcaggaga cgcttgagcg cgtccaacgc ttcgggcgca gaggcacacg
     1261 ccgcgacacg ctccgcgccg ctctgttcaa gcgtcgtcac gagcagcgag cgcgtatcgg
     1321 gatcatcctc cacgaggagc acgcgcagtc ccttgagcac accttcgcga ccgttgcggg
     1381 ctgccgcgtt aaacgcaccg tcggacgtgc cggatgcgtg gcgcgcgtcg gcgaggccgt
     1441 gcgggtctgt gctgtgtcga acccccacga gcggaagcct gacgcgaaag gtcgagccga
     1501 ggttcgcgcc gtagcttgcg gcgcgaatcg tgccgccgtg aagttcgacg aggtgacgca
     1561 cgatggcgag gccgaggccg aggccgccgt gcgcacgcgt gatcgaaccg tcggcctgac
     1621 ggaagcggtc gaagatgtag ggcagaaact ccggcgcaat gccctgcccc gtgtcgctga
     1681 ccacgatctc gacctgcgaa tcggaacggc gcagttcaat cgtcacgcgc ccgcccttcg
     1741 gcgtgaactt gatcgcgttc gagagcaggt tccagatcac ttgctgcaag cggtcggcgt
     1801 cgccggagac ttgtcccgcc gtcgtatcga ggagcatctc cagttgcacg ccgcgcgcgt
     1861 cggcggtcgg gcgcagacta tcgacggcgg cagccatgat gggcgcgagt tcgagggttt
     1921 gcgggacgag gcgcagtttg cccgtgatga tgcgcgacac gtcgagcaga tcgtctatca
     1981 attgcgactg cgcgaccgcg cttcgttcga tgatttcgag cgcgcgattt gaaagcgtcg
     2041 gatcgaactt ctccgtgcgc atcagtttcg cccaaccgag aatggaggtg agcggcgtgc
     2101 gcagttcgtg cgagagcgtg gcgagaaatt cgtctttcag gcggttggct tccgcgagtt
     2161 gttcggcgcg ctggcgcaac tgttcttcga gccgtttgcg ctcgatgccc tgcgcgatgg
     2221 tttctgcaac taaggccaga gagtcgagcg tctcttccgt caaatgttca cgcgagaaga
     2281 tcgccatcac gccgatgagc ctgtcttcga cgagcagcgg gtaaccggcg aaggcgacca
     2341 tccccgtgcg cctcgcccat tccttgtcgc tgacgcgcgg atcgttcagg acatcgttgg
     2401 tgcgatgcgg ctttcgctcc tctgcgatga ggccgatttt gaattggccg aggggcacgc
     2461 gcgcgtgcgt tccgtcgagg cgcgtgtaga tgcccgcgct ggcctgaagt tcgaggaagt
     2521 tggtgtcggg gttgaaggtc cagatgcgtg cgaaggccgc gtcgaagtgg ctgacgaggg
     2581 cttccgcgct ttcgcgcagg acgctctgca agctgtcgct cttcgccagc gcgacgctga
     2641 catcggcgcg cagagtcgtc tggcgtgcgc gttgtgcgcg tcgctcctcc gcacgcttgc
     2701 gctccgtgat gtcgtgcgcg gcgagcaccg cgcccgtcaa cacgccgtca accatgatgg
     2761 gcgcgcacga gcagaagatc gggatcatcg tcccgtcttt tctgatccac acgtcttcgc
     2821 ggttcgtgat cgtcgccttc gagttcaaca cgtcgccgag cgggcactcg gagaggggaa
     2881 aaggcgtccc gtcgggtttc aggtagtgca ctgcatgatg gaggacgcgc cccttgagtt
     2941 ccgcttcctt ccaaccgagc atcgcctccg ccgccgggtt cataaaggtg aggcgacctt
     3001 ccgcgtccat caggcacaga ccttccgcag tgttctccgt gatcgcgctc gtcaggttca
     3061 gttgataacg caaatcctct tcgacgcgtt tgcgctcgct gatgtcttcg tgcatgagga
     3121 cgacttcgcg aatctcgccc gccgcgtctc tcaccgggta gatgatcgcc tgcacccaac
     3181 gctgcgggtg ctcgttgacg gtgacgttcg ggatggtgac ttccgggtcg tagagaatgg
     3241 gcgggatgga ggtcggggtt ccggcaaagc ctttttggat gtagggcatg atgccctttg
     3301 cgacgagttg cgggtcttcg aggatgttgt agtcgccgag cgtttcgagc gtgacgcccc
     3361 agagttcttc ccaagcgcgg ttgacctgaa tcgtgcgacc atcgggcgag aagacctgcg
     3421 tgctcagcgg cgactgctcg atgagcgtgc gaaagcgttc ttcggattga cgtagtttcg
     3481 ccgccgtgcg tttcgccgac gcacgcgctc tcgcctgcgc gcgcgtcaac cagaagagca
     3541 cgaagcccgt gagcaggccg cccagcagga tgtaggtcag gtggccgccc gcggtttcaa
     3601 ataactggcg ccctttctct tcggccttct gcaccacgta gtagatggag aaggccgtca
     3661 ggagcagcgt cacggcgagg acgacatagg gagtccatgc ccgctcgcct tcatcttgtt
     3721 caggagagaa ctgctgtctc atctgtcgct taacactcct gcgcacgcac gcatgcacta
     3781 ttgttcgact taagacggcc tcgccgccaa ctgtcaacgc ctgttgtaac ctattttcta
     3841 acgtcgtgag tccgcctgtt gtctcgccgc aacctgctct tgaaagtgat gctatcacag
     3901 gcgtctgaat tattgcgcgc ggggcggcga cgaattgagc ctcgcacgtt ttcgcgcccg
     3961 gacaaaaaaa gccgcgagac aactttcagt gttgcccgtg gctttttgct tgagaagatt
     4021 gagtcgaaac ctgcggccag ccgcgcttac gtatcacgcg cgacgcgctt acgaatggcg
     4081 cacgccgcgc cgctgtattg ttcgtatctt gatagggtga tgcacgatgc gcggcgcttt
     4141 gggagcgagc gacggcagca gcggcgtgcc cgctttgccg gtggaggaag atgacgcgga
     4201 ggctgccgca acgtgcgcgt gagtctccgt ctgcgcttcc gccaacagtt ccggcctgtg
     4261 cctgagagaa tgcgagcgca gttcttccca ctccgcaggg ggaatgcgca gaaaggctgc
     4321 gacgacatcc gggtcgaact gctgccccgc gtgtcgcttc aactcctcgg ctgccgcctc
     4381 gtaaggtttt ccggcgcgat agacgcgcgt gctcaccatc gcgtcgaagg catccgcgac
     4441 ggcgaagatg cgcgcgttga ggtcgatctg cgcgccgcgc aagcccgagg ggtagcccgt
     4501 cccgtcccaa cgctcgtgat gctggccgac gacgcgcgcc gcgccttcca gaaatttgat
     4561 gccgcgcaaa atctgcccgc cgagcgcggg atgctgtcgc atcgtggtcc attcttcgtc
     4621 ggtgagcgcg gcgggcttgc gcaggatgca gtctggaata ccgatcttgc cgatgtcatg
     4681 caggagcgac ccgaactcaa gcgcgcgtaa ctgtgcttcg tccagatcga gttcgcgccc
     4741 cagccgcagg ctgaaactga cgacgcgctc cgaatggccg tgcgtgtccg tgtcgcgcgt
     4801 ctccaaggcg gcggcgagag ctttgagcgt cgttcggtaa gcgtcttcga gcgaggcgag
     4861 ggcttcgccg agttccttcg tgcgcagctt gattgactct tcgaggaaga gttcgtaacg
     4921 gcgtttgctg cgcaacagtt cgcggtgctc gcgcgcacgc tcgacggcca tctccacgtg
     4981 gcgcaggtca aaaggcttcg tcacgtagtc gaacgctccg gcgcgcagag cctcaatcgc
     5041 gctctccacc gtctgcgcgc cgctgatcat gatgatcacc gtctcgggcg cgatctgacg
     5101 cacgtgcgga accatctcca gcccgctcat cccctccatg ttgatgtcgc tgatgatgag
     5161 gtcgaaattc gacgtggtga gcagggcgag cacctcttca gccgagccta ccgcgcggca
     5221 atcgtaatgc tcgcacaaac actcttgcag aatacttctg atctccggct cgtcgtcggc
     5281 gataagcaga cgcgtcttac gtctcggttg ttcagacctc atggctgccc ctttcgaaag
     5341 aaactaccgt ccacaaaaga cgcgtccccg ccaccctggc gcgtcgcttc cgccccaaaa
     5401 agcatttact tgttcaaaag ttaagggtgt tgaaccaaat ccattcaaac ggggagtgta
     5461 cgacgggcat cattcgcaag gggcgtgcca agtctctgcg ggggcgcgtg cgcctcgcgc
     5521 gttgcgaatt gccgtggcgt cagggcaaat cgtcggcgtg cgcataaagt taatgctgcg
     5581 gcgcaactca gagcgcgcaa gtctccgtct ctaaacttct tgataaaacg cgcgccatcc
     5641 gcccgcgcct cccgcacgcc gcgcgcacgc acgcgtccgt agcaacttcc gccagacaaa
     5701 gaatttggcg gcgcgcggca aatcactgcg gcacgatccg cgcgacgccg cattatgcgt
     5761 ctggcttcac gctccgtcgc aattttggct ggaacgaatc cattcaagga tgttccgtct
     5821 tcaaagcggc gaggctcccg cgcgttttcg cccgcgcgtg ctgccatcgt gcgcggcttc
     5881 ttcttgagca tcagcgacat acgggcggcg tcgggtgacg cgtcgcggcg tcggttcaga
     5941 aatcgcttta agttatttca aaggctggcc gcgcccgcgc gcccggttca gtagagaggc
     6001 gcggcgcgag gtacgactct tgccatagaa gataggaagc aacagacaga aggactccgc
     6061 gccggagaaa ccctcaagaa gaaatcgaca aagttggaca ggatacgctc agaggaggag
     6121 ccgaaggtga caagcgtaca gcatttc
//
LOCUS       DASWNS010000090         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_289108, whole genome shotgun sequence.
ACCESSION   DASWNS010000090 DASWNS010000000
VERSION     DASWNS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289108"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VGB76_03100"
     CDS             <1..291
                     /locus_tag="VGB76_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097920.1"
                     /translation="DTAATEEFWQLINERPEIAALFIESSFPNSMGKLAEVSGHLTPE
                     GVAGELRKLAHNHFDVLAVHLKPSYRETLVKELDALGIRGLSAMEVGRTYEW"
     gene            complement(373..717)
                     /locus_tag="VGB76_03105"
     CDS             complement(373..717)
                     /locus_tag="VGB76_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0097921.1"
                     /translation="MSQERIEAFKAMVEQQPEQEMVWYGLANEYLKLERWDEAADALG
                     HVLKLNADFTAAYQMLGTALMNGGQREEARRVWAEGVEVANRTGAWKARQHMEGLLAD
                     SEAAKAGGEFCE"
     gene            complement(780..1091)
                     /locus_tag="VGB76_03110"
     CDS             complement(780..1091)
                     /locus_tag="VGB76_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097922.1"
                     /translation="MKTTAKRLLGGLALAVGGLGAVVLLKGRKREAARGGEEKAQSRE
                     RGQDRWARPGMSVTFRGELMPGRQRAERTFRVVELLPSGRVRLDGVAGEHSEHEFEAA
                     R"
     gene            complement(1255..1641)
                     /locus_tag="VGB76_03115"
     CDS             complement(1255..1641)
                     /locus_tag="VGB76_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEY0097923.1"
                     /translation="MFYPHALPSRPHAVGTEAKFLCGYRHINLEEASLMTYVVCEPCF
                     GCRYTDCALVCPVEAFHLDDEMAALVINPETCIDCDACVPECPVEAIFAEDKVPEEWA
                     HYTEINADRSLRAGLPIQSEKLDPLC"
     gene            complement(1826..2002)
                     /locus_tag="VGB76_03120"
     CDS             complement(1826..2002)
                     /locus_tag="VGB76_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097924.1"
                     /translation="MTSNSQKPSKKRPGNTLYDTIRKPVAPPGHPLTRAKPEERAHPA
                     ERKVKHKRRAADEG"
     gene            complement(2237..2470)
                     /locus_tag="VGB76_03125"
     CDS             complement(2237..2470)
                     /locus_tag="VGB76_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097925.1"
                     /translation="MTTNKSIKIIKRGQRAAQEAAEASAAQSEQKKSAHEAARDVVAT
                     VTEWVNEFQQKRRTETTHALKTLFGETPQPTKA"
     gene            3031..3948
                     /locus_tag="VGB76_03130"
     CDS             3031..3948
                     /locus_tag="VGB76_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097926.1"
                     /translation="MLLSEFIIIYLAAAAPFGVTNFLRQPAGARRTRSLLHATGAALL
                     WPLTLLSLPFVHKKFRRVEPEAAAPEVSLSREQRTEVARRALLSSLYRMEDLAEEVPG
                     LQTEATRSALRETISAVERYVGLALAVAEADEAASVRVPRETELCRLSGRAGDDLHIA
                     TLCHGRRNIARLRAHGADSRLELLHALAELREVFESTHAATPTEAQAVAPSRHLFAAL
                     LETYMRAIDLLSLFEDERAAATVVRLLDAVRARLRRSESLDAEAAPPSLMQTDRESNV
                     TGDEPCITSIPQTNPQTQLSPTQTIRVHG"
BASE COUNT          737 a         1297 c         1141 g          855 t
ORIGIN      
        1 gacacggcgg cgacagagga gttctggcag cttatcaacg agcgaccgga gatcgccgcg
       61 ctcttcatcg agtcgtcgtt tccaaactcg atgggcaaac tcgcggaagt ctccggccac
      121 ctcacgcccg aaggagtcgc gggagaacta cgcaaactcg cgcataatca tttcgacgtg
      181 ctcgccgtcc atctcaagcc ctcctaccgc gaaacgctcg tcaaggaact cgacgcatta
      241 ggcatacgcg gcctctccgc gatggaagtc gggcgcacgt atgaatggta gacgggcaac
      301 gtgtaaaaac taaaagccgc ttccggtaga aacggctcac ggatagttga ttgagtttta
      361 agcgtcagtc catcactcgc aaaattcccc gcccgccttc gccgcctccg aatcagccaa
      421 caagccttcc atgtgctgcc gcgctttcca cgcgcccgtg cggtttgcca cctcgacccc
      481 ttcagcccac acgcgacgcg cctcctcgcg ctgcccgcca ttcatcaacg ccgtgccgag
      541 catttgatag gccgccgtga aatccgcgtt cagtttcaac acgtggccga gcgcgtccgc
      601 cgcttcgtcc cagcgttcca atttcaggta ctcgttcgcc agcccgtacc agaccatctc
      661 ctgctccggc tgctgttcga ccatcgcctt gaacgcttcg attctttcct gtgacacttt
      721 gactccaacc cttccggcta cgtcggggca aaaacgcgct gaccgcgcgc ccgagcgcgc
      781 taacgcgccg cttcaaattc gtgttccgag tgctcgcccg ccacgccgtc gagccgcacg
      841 cgcccgctcg gcaacagttc gacgacgcga aacgtgcgct ccgcgcgctg gcgacccggc
      901 atcagttcgc cgcgaaaggt gacgctcatg cctggacgcg cccaccggtc ctgcccgcgc
      961 tcacgcgact gcgctttctc ttccccgccc cgcgccgcct cacgcttccg ccccttgagc
     1021 aacaccaccg cccccagacc gccgaccgcc aacgccagac cgccgagcaa acgtttcgca
     1081 gttgttttca tccctatcct gccgttctcc ccgccgccct caaacgttcg ccgccgcgtg
     1141 cgcctcagaa acgatgaagc gcaagcccct ccgcgcgttt gagtggcttg cgcgccgtct
     1201 atcgtcgtgt cgcgcgctcc ggcgagcgcg cgctcgtcat atccttacgg agtctcagca
     1261 caatggatcg agtttttcgc tctggatggg caaaccggcg cggagcgacc tgtcggcgtt
     1321 gatctccgtg tagtgcgccc attcctcagg gactttgtct tcggcgaaga tggcttcgac
     1381 gggacattcc ggcacgcacg catcgcaatc aatacaagtc tccgggttga ttacgagcgc
     1441 ggccatctcg tcgtcgagat gaaaagcctc gacgggacag accagagcgc agtcggtata
     1501 acgacagcca aagcacggtt cgcacacaac gtaagtcatt aaactagcct cctcaaggtt
     1561 tatatgacga tagccgcaca aaaacttcgc ctccgtgccg accgcgtgcg ggcgcgacgg
     1621 cagggcgtgc gggtaaaaca tgaaaaatct acaagcgcgc ttatattaag cacgaaccgg
     1681 caagattgaa aagcaaaaag ttacgtccgc aggcgcgcct gttgtgaacg gtcaatcgcc
     1741 aatcggcggt tgtctcctga ccgccgggcg agttaccaaa gattgaattg aacaacgcac
     1801 aactgacagc ggacggacgg cctttttacc cttcatcggc agcccggcgt ttgtgtttga
     1861 ctttgcgctc ggcggggtgc gcgcgttcct ccggcttggc gcgtgtgagc gggtggccgg
     1921 gcggcgcgac gggcttgcgg atggtgtcgt agagagtgtt gccggggcgt ttcttcgacg
     1981 gcttctggga gtttgaggtc atgatgttcg aaagcctctt ttttggggat gtggcgcgat
     2041 gctacgcgcg ctccccttgc gtgtcaatag ccgtgcgtgg gcgcgtgttg gagttcggac
     2101 tcttttcgcg tgagtgtgga gggggtgtag agattgagag gcgtgcgcgc gggtaggagg
     2161 ccgtaaaaga gtttgcgcct gagcgttccg ttgagaaacg ctcaggcgca agaaattagt
     2221 ttcggctgtc gtgaatttac gccttggtcg gctgcggcgt ctcgccgaag agcgtcttca
     2281 aggcgtgcgt agtttcggtg cgccgcttct gttgaaactc gttcacccac tccgtcacgg
     2341 ttgccaccac gtcgcgcgcg gcctcgtggg cagacttctt ctgttcgctc tgagccgcag
     2401 aggcttcagc agcttcctgt gcggcgcgtt gtccgcgttt gatgatcttg atggatttgt
     2461 tcgtcgtcat gattgcgtta cctgcttgtt tattttcttt cgttctataa gacgctcaag
     2521 gcaccgaaaa agttccaatt tgttgttcgc ggtgccgtgc ggcttgagaa cttgtctctc
     2581 tgcttagcaa atcaaaggcc acaactttca ggtcgagccg ggagccttcg cggatgaggc
     2641 gaagcgaaaa ccgcgtgccc gtttttgctt ggtttctcac gcgctttaca ttcgctctac
     2701 acgcgcgcaa cttcgccgtg acaattcaac cgttaagccc actgccttat atagtttctc
     2761 aatcgttcac tctcaaccca tctgcgactt tttctgatac gctttcggac actgttaact
     2821 cgtccacgct ctttgcagaa tgccaacgcg acgctccggc ttgcgcgctt tgccaatcgc
     2881 acgctccttc ctcagaaccc gcgcgccgtc gctcgccgcc ttcgcaaaaa taactaacct
     2941 tattgctgtg aatgaatggc tcgcaggagt gccctgtgtg ttggttctca cgcgcgcgtt
     3001 ggcatgttcg ttgccctagc gtcaggcgtc atgcttttaa gcgaattcat catcatctat
     3061 ctcgccgccg ccgcaccctt cggcgtcacc aattttctac gccagcccgc aggcgcaagg
     3121 cgcacgcgct cgctcttaca tgcgacgggc gcggcgttgc tctggccgct cactctgctc
     3181 tcgcttccgt tcgttcacaa gaagttcaga cgagtggaac ctgaggcagc cgcgccggaa
     3241 gtttccctct cgcgagagca acgcacggag gtcgcgcgcc gcgcgctgct ctcgtccctg
     3301 tatcgcatgg aagacctcgc ggaagaagtt ccgggtttgc agacggaagc gacgcggagt
     3361 gcgctgcgtg aaactatctc tgcggttgaa cgctacgtcg gccttgcgct ggccgtcgca
     3421 gaggcggacg aagcggcatc cgtgcgcgtt ccgcgcgaga cggagttgtg ccgcctcagc
     3481 gggcgcgcgg gcgacgacct gcacatcgcc acgctctgcc acgggcgacg caacattgcg
     3541 cgtctacgcg cgcacggagc cgattcccgg ctcgaacttc tacacgcgct cgcagagttg
     3601 cgcgaggtct ttgaaagtac gcacgcggcg acgccgacgg aggcgcaggc cgtcgcgccg
     3661 tcgcgtcacc tgtttgccgc cctgctcgaa acttacatgc gtgcgattga cctgctctct
     3721 ttgtttgaag acgaacgcgc cgccgcgacc gtcgtacggc ttctcgatgc ggtgcgtgcg
     3781 cgtctgcgtc ggagcgaatc tctcgatgcc gaagccgccc cgccatctct catgcaaacg
     3841 gacagggaga gcaatgttac cggagacgaa ccatgtataa cgtccatccc gcaaacgaat
     3901 ccgcaaacgc aactttcgcc cacgcagacg atccgtgtac acggctgaac tggctcaccc
     3961 gatggaacgc ggaggccgaa cgccacgcgc gccttttccg cagcgcacgc gaagaggagg
     4021 agttgagcct
//
LOCUS       DASWNS010000091        20684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_29383, whole genome shotgun sequence.
ACCESSION   DASWNS010000091 DASWNS010000000
VERSION     DASWNS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20684
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29383"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..541)
                     /locus_tag="VGB76_03135"
     CDS             complement(74..541)
                     /locus_tag="VGB76_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097927.1"
                     /translation="MSYQREAHEEAWMGATVIVYWPDITEEQIDSQPDFYNDSKAWGD
                     WMAERESEPAVTDALRKLNAEAILTQTTDGVRDEDVMWVTPAQLRDAATRLREAVRGG
                     STETRIILETYARNAGSEEEVAEDFVRDLEDIIEMAGWAEKEGASKITLEVNW"
     gene            916..1719
                     /locus_tag="VGB76_03140"
     CDS             916..1719
                     /locus_tag="VGB76_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097928.1"
                     /translation="MSKQINRLLGRTRLLVLTIAVIAAVCVATVALAVNSEPGSAAVN
                     TGAAAPADAGSSHTQGRGSFAAYTLEQRQTFTPQGGEPFVNAKKERYQRSDGVFLLVH
                     TYYRPDGSVITVETGLGYLGSGAYRVDEKNNLLEFVAVQDSDAPTATEEQLRADPGFE
                     QEETLAGQRTLVARYSEKGAAANYRQVYRAPELQNLALKTVIVSPRGEEIIEPVRLDP
                     GEPPATLFDKALRYRKSYARYEERIRQTEQKGDVERASMMRKHLARVRG"
     gene            1879..7737
                     /locus_tag="VGB76_03145"
     CDS             1879..7737
                     /locus_tag="VGB76_03145"
                     /inference="COORDINATES: protein motif:HMM:NF019227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HEY0097929.1"
                     /translation="MRTNLVSSSLSAALRVLSHSFRSLSRRSARTVAACCAALLLLCA
                     GVRAQTVEHTENRADQAIRSEARIDPSTHALELAVPLRNIPGRGGMGLPITLNYSSKV
                     WRIQYISGHSTYLNYHTWSSAMYAEDSAAGWTTSIAVPRIEYTGQEQYYSFSGQPKGD
                     DLEGGDDPYQPSYYLARINVKMPDGSSHDLLKDYVARVLGTGGYDYTGMFYATDGSHV
                     RYDNNTNTLYLPDGSRYILGDPDGSREVYGLTSPPTYYIDRNGNTRVYNHQTKQWTDT
                     LGRSIGQLKDPSGAVVGGMPLPANPQVGNYTYTLSGVGGQAINYTLRWSNLSGALTNP
                     NQTLHYKGSHQCGSTYTAVSPHLFSSSYPNYVCYGGVFNPVVLAEIALPDGHSYRFTY
                     NIYGEIDKIYHPGGGYERVRHEQKTPLSHMDSPYSQGNRGVAERWVSVKGDGTDEARW
                     EYGVSTGQYNSAPYATYVNLPDGSRSERVMHMGSTGGPASPPFGIEDARVGMAYEERS
                     YSAPDQYGNRQMLRRTLTEWATSPVNVTDESGYPMASVTRAARVVKEVGLLLDTGGAA
                     LASTVTYSHDQDLNVTATNRYNYTTVAASTAQTAAIGSIPTGTLDRTDEVTYLVNDPS
                     VSAGTRQAYRDRNLLGLPSSTRVKNGATALVAQSEIKYDEAAYPLVIYGAVTGWTEPG
                     TNVRGNQTTLRSWIDTSNTWIETHTQYDQCGNARSIWDAKGNQSQLSYADSFSDGVGR
                     STYAYTTQTISAVPDPSNTRATNTPLVASTVYDYWTGIKLSTTDANGKVTTYQYNDAL
                     NRLSNVNHPDGGWSAFWYGRNAYGDYIGTRQAINATQHTESYQFFDGLGRPVRSFALD
                     GSQWITTDTQYDALGRVWRVSNPYLSGGSGTQINPSGNWTTRSYDALGRETSLTTPDG
                     AVTNNAYSGNQTTVTDQAGKQRRSITDAFGRQTQVIEDPNGVAYVTNYTYDPLGNLRR
                     VEQGGQYRYFMYDSLGRLLRVKNPEQAANANLATAADPVSGNTQWSLGYSYDNNGNVS
                     VRTDARGVTTTYQYDNINRLKQTNYSDGTPYTLRTYDFATNGRGRFYADYESSTSGTL
                     NFVLTYDAMGRPTTGKTEFYLLGWGWRPAYTMARAYDLMGNITSLTYPSGHTAQYPQH
                     DVLGRLKKFNGNLGDGVQRTYATGINPANGVEEGIQYNESGRIRQERFGTQTPLYHKQ
                     QYNLRGQLSDIRLGLGYDVWSTERGAVVIDYGTTLNNGSVVSQQYWIPTDTTATAWDV
                     RQTNYAYDNLNRVAGMQEFYGPAGMVMQQWFTYDRWGNRSISPSTTDGLNELAFDVDE
                     TSNRLYAPGQKNLPDSSRTLRYDAAGNVTMDVYSAHDALRTFDAEGRMTAAHDSQSAR
                     VYRYTYDAGGRRMRRDLSGQETWQVYGFDGELLAEYAINAAPTQPQKEYGYRNGELLI
                     TAESGGQVNWLVTDHLGSARMIADLSGSLAGMRRHDYLPFGEEAFAGQGGRTTGQGYA
                     SDAVRQKFTGYERDSETGLDYAQARYHSPTQGRFISPDPLLSSGVPANPQSWNRYTYV
                     LNNPLALVDPSGLIWGKKDGDDHIRWFNSEAEMKAAGYEAIDKFYYERDGTWYAVDPN
                     SNRYYKSTDGRMAQKAFWSFLGLPDSNWDVLPGVRSVRSFFFYYATGDYNKAMGSFAL
                     ASVEGGTSASGVKSLFNLGAKGIKSIVASLTDDAGRALADDAVKHANCFVAGTPVHTI
                     EGLKAIEEIKPGDQVLSWNEKTQRVEYKTVVRTFANRASALVVLSIAGESTPLTTTPG
                     HPFYVRRARDSLKVADEDDGAWILAVSLRAGDEVRRPDGQWVRIDDVTQRAETTTVYN
                     FEVADNHTYYVGDAGVLVHNQSVLRTVIEDDAAAAAADKMRGNVASAYDELKNLLSSG
                     RPGKNQHPLTGEYAGMMAADLTGSGKGRGAMRVIFSRNDNEVIIHKIVDYHK"
     gene            7808..8041
                     /locus_tag="VGB76_03150"
     CDS             7808..8041
                     /locus_tag="VGB76_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097930.1"
                     /translation="MSTWNYRVARRNGEYGVYAAYYNDDGTIVSLSRDPLSPTGDSPD
                     ELRTQLELLLESLDAEVVDYASIADNREKPDDL"
     gene            8447..8686
                     /locus_tag="VGB76_03155"
     CDS             8447..8686
                     /locus_tag="VGB76_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097931.1"
                     /translation="MKPREAIHQALYLALVEIRAEAYTAENNKIFHLADLFHNIPLQL
                     ERVSQDEESYDDVLEWLRKRASEKGFLQWLDQAIE"
     gene            8705..9130
                     /locus_tag="VGB76_03160"
     CDS             8705..9130
                     /locus_tag="VGB76_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0097932.1"
                     /translation="MSENRFGKLEAEGLAFLHAGQFADAIEVFMKLAEENPGYEHGIC
                     FYHLACCFEDVGDFEKARLHYERALQAAPGDVYRVGGYAAFLYLHGNPKDAFEAQLNY
                     LRVVRRMGNKTDEEKAISNLKHLGAKLGLTEDEIMRTGQ"
     gene            9490..10026
                     /locus_tag="VGB76_03165"
     CDS             9490..10026
                     /locus_tag="VGB76_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEY0097933.1"
                     /translation="MALGLAMDAFAVSVADGARARQRRFSHAFQLALTFGLFQALMPL
                     IGWTVGLGFKDIISSVDHWIAFGMLGLVGGKMIYSDLSAKAAAAPESRVPGGVTTLLV
                     LAVATSIDALAVGFSLTFLPSILLPVLIVGVVTFSTCLAGVHLGQRYRHFGRSKVQTA
                     GGAILIAIGIKILIEHLS"
     gene            complement(10099..10230)
                     /locus_tag="VGB76_03170"
     CDS             complement(10099..10230)
                     /locus_tag="VGB76_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097934.1"
                     /translation="MSGNGGHVGLRWTPAPSTTLTFDADATQRSVKLGLNQATLSTK"
     gene            complement(10433..10648)
                     /locus_tag="VGB76_03175"
     CDS             complement(10433..10648)
                     /locus_tag="VGB76_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097935.1"
                     /translation="MDVPTLAALLRETAERHDRYERTHAEHHWWDWYASYLSARQQGS
                     SSEEAEAAADRYMEEVSPCSSPMKPCD"
     gene            complement(10658..11140)
                     /locus_tag="VGB76_03180"
     CDS             complement(10658..11140)
                     /locus_tag="VGB76_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0097936.1"
                     /translation="MIEVKKAQVDMKLEVVVIPVSDVDRAKAFYGNLGWRLDIDFTVG
                     DDFRGVQMTPHNSEASIIFGKGSTSAKPGSLEGMVLAVDDLDAARDELIARGVDVSEV
                     FHYAGGPFNNTGKNPRVGGRDPEGRSYFSFASFEDPDGNGWLLQEITTRLPVRTWKRE
                     "
     gene            complement(11181..11705)
                     /locus_tag="VGB76_03185"
     CDS             complement(11181..11705)
                     /locus_tag="VGB76_03185"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEY0097937.1"
                     /translation="MKRIIFLLVAVATLGGVVAFKATASRHADKEAAAVFVKTIPPGY
                     RDWRFISVAREEGELDDVRAILGNDVAIKAYREGKLPFPEGTIIARLAWSFDPSEENN
                     KVFGRRQSFVAGHPKNGVQFMVKDSKKYAATGGWGYAQFDDGKPAADSLMNTCFACHA
                     AIKERDYVFTHYAP"
     gene            complement(11787..12128)
                     /locus_tag="VGB76_03190"
     CDS             complement(11787..12128)
                     /locus_tag="VGB76_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097938.1"
                     /translation="MLDNFTLYWLTNSAISSARLYWENGGRSVTSATAQKTTEISVPV
                     AVTVFPDEVYRAPETWVRRAFRNLIYYHEADRGGHFAAWEHPQLFSEELRAAFRSLRQ
                     PRPAAPVQGCP"
     gene            12460..12831
                     /locus_tag="VGB76_03195"
     CDS             12460..12831
                     /locus_tag="VGB76_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0097939.1"
                     /translation="MIVSTPLISVVDDDESVRESLPDLLREFGFAAQTFSSAEEFLAS
                     DFIGQPKCLVLDIAMPGMTGPDLQQELARRRQEIPIIFITAHADETVRPRMIERGAVE
                     CLFKPFSETALLEALNAAFRE"
     gene            12873..13538
                     /locus_tag="VGB76_03200"
     CDS             12873..13538
                     /locus_tag="VGB76_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0097940.1"
                     /translation="MNAARLSPESQDKPIIFVVDDDISVRESLELLIGFAGWQPEVFA
                     SAEEFLAHPRVHAPSCLILDVSLPDLNGLELQKLVAGDRTDMPIIFITGYGDVPMTVQ
                     AMKAGAVEFLTKPFADDVLLGAIQHAIERSESSLGKEAETQVLRDSYASLTPREQEVM
                     VLVVSGLLNKQIGFKLGISEITVKAHRGKLVQKMKADSLAGLVQMAVKLRLAPARNPW
                     QKQ"
     gene            complement(13709..15331)
                     /locus_tag="VGB76_03205"
     CDS             complement(13709..15331)
                     /locus_tag="VGB76_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0097941.1"
                     /translation="MTADTHHGRDDHTSDAARTRTALLAHIRHELSSPINAIIGYGEM
                     LVESATEEGAGEFRADLEKILTAGRQLRTRVSDLLNPARTETPDLDLDSFGAHVRHEL
                     RTPINAVIGYSEMLIEDADAPEHAEFVADLQKIQTAARQLLELIEDIVRFSDLEADSA
                     TLRPADDGVSSMIANLVSTIHAIDNAHARQTEQGGLVLVVDDSETNRDILARRLTREG
                     YEVETVPDGARALEAVGLRRFDLVLLDIMMPGLNGYQVLERLKTTPELRDIPVIMISA
                     LDEIDGIVRCIEMGAEDYLPKPFNPVILRARVEASLEKKRLRDREQAYLAQLRIEREK
                     SERLLLNILPSAIAERLKEAQGVIAESFTEATILFSDVVGFTQMSARITPVELVYLLN
                     EIFSSFDELATRHGLEKIKTIGDAYMVAAGLPERRADHAEAMAEMALDMQAALAAFNR
                     ARGTTLNIRTGINTGPVVAGIIGTSKFIYDLWGDAVNTASRMESHSTPGRIQVTASTF
                     ERLRHAYLFEPRGTINVKGKGDMSTYFLLGRQ"
     gene            complement(15328..15705)
                     /locus_tag="VGB76_03210"
     CDS             complement(15328..15705)
                     /locus_tag="VGB76_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0097942.1"
                     /translation="MKKILLVEDNEMNRDMLSRRLVRKGYEVSIAVDGRQGVEMAQSG
                     APDLILMDMSLPVLDGWEATRQLKNADATRHIPVIALTAHAMSGDRERALEAGCDDYD
                     TKPIELPRLLEKIETMLNRSAAS"
     gene            complement(15745..18036)
                     /locus_tag="VGB76_03215"
     CDS             complement(15745..18036)
                     /locus_tag="VGB76_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0097943.1"
                     /translation="MNRVLHRYRFTLVFLLIGVIVVGVGWYVVSDLLRAKRQVQQMYT
                     GTVQGIDLIGDLLYSTQETRRTMLYALTTDDPNLQIVYADQSHEAGKRIAQLITAERL
                     HAKSQQLLDAIDMFERDWAHYLKNRDEVIGLILQGSIKEAAESDQKNGIPAFNQVRDD
                     LLQIEQLYKADAENQLTSVNRSFDNTLYKLVTILFVTLLLAVVVIRTIQRGRMLRAMK
                     RANDELSDALRQLQETQAELQLAKEEAESANQAKSTFLANMSHELRTPLNAIIGYSEM
                     LQEDAADTGQDEFVPDLQKIQTAGRHLLALINDILDLSKIEAGKIELYLESFDLSALV
                     GEMESTIKPLAAKNENALIVHADPELGTMYADLTKVRQSLLNLLSNACKFTKAGNVTL
                     EVVRSREDGREWINFSVKDSGIGITKEQMNKLFQPFSQADASTTREFGGTGLGLVITK
                     KFCEMMGGTMGVSSVRGEGSTFVIKLPADVRSESPDTNAPAPGGEGRAELLDEDAGMV
                     LVVDDDPVARDLLQRALGKAGFRVECATDGEEALQLARTLRPEAITLDVMMPGIDGWA
                     TLAALKADPALADIPVIMLTIVDDKNKGYALGAADYMTKPFDREQLAAVLAKYRRAAT
                     NRNAPALVVEDDEATRALLVRGLKQEGWQVREAANGRAALAQVAEEQPQLILLDLMMP
                     EMDGFEFVQELHKLPGARSIPVVVITAKDITLEDRLRLSGYVEKILEKDSYSRDDLLR
                     AVREMVEVSVRSRGSDSLVKLPS"
     gene            complement(18085..18630)
                     /locus_tag="VGB76_03220"
     CDS             complement(18085..18630)
                     /locus_tag="VGB76_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097944.1"
                     /translation="MRVRVFGLAVLLTLLLASSRLAVWSEAAGGEAARAFASTRSPAS
                     LQTGDVAIIVNPKNSMRDISFDVLRKYFKAERGQWPNGTKVVIAMRQPPGQSERGAVL
                     RSIYGWDENYYQKYFRQGQFNESIQQAPKELNTTYAMIQYVHYTPGAIGYVRADQVDN
                     SKVNVLSVDGRKPGQPGYRIR"
     gene            complement(18642..20342)
                     /locus_tag="VGB76_03225"
     CDS             complement(18642..20342)
                     /locus_tag="VGB76_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097945.1"
                     /translation="MKHTPSVSSVLSLVLFFVCACPFAVPLHAQEPERLAASASAHDN
                     FAKAHRAPTPPVARPRKRLVPVRVSDTPEGSRVIVASDASLDNHYSYFDDGRFYVLIP
                     KADVAATMPDEFKGRGFASARLAQRGGDALLSFELQPGTTPRLSQKFNRLEINFTAQD
                     AARQPPAHAAQAVEQAQLQQLLKRVEELEGQVRELRANAVAANGQPPTVAPATTATAP
                     EAAAPEEEEAQAAITGQEHETHTSGPRLQIQGFADVNFRASNGRGTTNAFGLGQLDLF
                     ITSQLSEKFSVVSELILEAKRDNSFEFEIHRLLLRYAMNDYLNLSAGRYHSGIGYYNT
                     AFHHGSYFATAANRPFLFAFEGQGGLLPLHNVGVSATGRIPSGSLGLRYIAEVGNGRS
                     ARSAVDRTVQTAFDENNGKSYNLGLNARPDWLPGLQAGFSFYRDRLTPTAAPNVDQRI
                     MAAHVVYQSPRYEWLNEAVFIRHKPESAGGVATYTPGFYTQFARRFGKAQPYFRYQYL
                     NAPTRDLLFRDIGRRNGPSFGLRYDLTTYAAFKSQYDRTERRGLSTLDELILQLAFTF
                     "
BASE COUNT         4179 a         6095 c         6208 g         4202 t
ORIGIN      
        1 gctttatagc cttactgctc accgctcgcc gcatccgggc ttgctcgata cctttgagac
       61 gactgccgga cggctaccag ttgacttcca gcgttatttt cgacgcgccc tctttttccg
      121 cccatcccgc catctcgatg atgtcttcga ggtcgcgcac gaaatcttcg gccacctctt
      181 cctcgcttcc ggcattgcgc gcatacgtct caagaatgat tcgggtttca gttgagccgc
      241 cgcgcacggc ttcgcgcagt ctcgtcgcgg cgtcgcgtag ttgtgcggga gtcacccaca
      301 tcacatcttc gtcgcgcacg ccgtcggtgg tctgcgtcag gatagcttcc gcgttcagct
      361 tcctgagcgc gtctgtcact gccggttccg actcgcgctc ggccatccag tctccccagg
      421 ctttgctgtc attatagaag tccggctgtg aatcgatctg ttcctctgtg atgtccggcc
      481 agtaaacgat gacggttgcg cccatccaag cctcctcatg cgcttcgcgc tggtatgaca
      541 aaaactttcc gcccgcgacg gattttggga gtcattatac ttcttgccgc gggcaatttc
      601 ggacacgttt ccgccccgct cggtcgtaaa actttctcaa caatttcttg aatttctcgt
      661 gtagatttcc gaaaaagcag ttaggtattt tacgccgtcc tttggtgaac ctgcgataaa
      721 agactcactc tttcatcgtc ttcacaaaac tcgtcaaacc agaagaaggg ctgccgtcag
      781 accttcgcag atgaatcgtc cgcccgtcgc tcggcagcgt gcctgctccg gcggctttcg
      841 atgtgtcgat aaaattaaaa ggccgtgctg tgtctactct agcccgggcg cggcctctcg
      901 tggggaataa gttttatgtc taaacagata aacagattat tgggtcgcac tcgtttactc
      961 gttctaacca tcgccgtgat cgcggctgtc tgcgtcgcca ccgtcgcgct cgctgtcaac
     1021 agcgaaccgg ggagcgcggc agtcaacacg ggagcggcgg ctccggctga tgccggatcg
     1081 tcccacacgc aggggcgagg ctcctttgcc gcctacacgc tggagcagcg acagaccttc
     1141 acgccgcagg ggggcgagcc gttcgtgaac gccaagaagg agcgttatca aagatcggac
     1201 ggcgtgtttc tgctggttca tacctactac aggccggatg gctcggtcat cacggtcgag
     1261 acgggcttag gatacttggg ttctggcgcg taccgcgttg acgagaagaa taatttactt
     1321 gagttcgtcg ccgtacagga cagcgacgcc ccgacagcca cggaggagca gttacgcgcc
     1381 gaccccggct tcgagcagga agagacgttg gcggggcagc gcacactggt tgcgcgctac
     1441 tccgagaaag gcgcggcggc gaactacagg caagtctatc gtgccccgga acttcagaat
     1501 ctcgcgctca agacggtcat cgtctcgccg cgcggcgagg agatcatcga gccggtgcga
     1561 ctcgaccccg gcgagccacc cgcgacgctc ttcgacaagg cgctgcgcta ccgtaagtcg
     1621 tacgcgcgct acgaggagcg aatccgtcaa acggagcaga agggcgatgt cgaacgcgcg
     1681 tcgatgatgc gcaagcatct ggcgcgcgtc agaggctaga ggctaaagcc ccgcagtcga
     1741 aaacataact gagaggcgag tgcggcgtgc atcccgcact cgccaagcgg aatcatcgag
     1801 aagtctggcc gcgcccccat cctcaaactc ttacgcgcgg tctaaacatc aaccaatttt
     1861 tttagagaat taaatatcat gcgtactaac ctcgtctcgt cgtcgctttc tgccgccctg
     1921 cgcgtgctct cacattcatt ccgctcgctc tctcgccgct ccgcgcgcac agtcgcggcg
     1981 tgctgcgctg ctctgctgtt gctctgcgcc ggagtgcgcg cgcagaccgt cgagcacaca
     2041 gagaaccggg ctgatcaagc aatccgcagc gaagcgcgga tcgatccttc cacgcacgcg
     2101 ctggagttag ccgtgcccct cagaaacatt ccggggcggg gcggcatggg gcttccgatc
     2161 accctcaatt actcttcgaa ggtgtggcgc atccagtaca tctccggcca ttccacttat
     2221 ctcaactatc acacctggtc gtcggccatg tacgcggaag actccgccgc cgggtggacg
     2281 acgagcatcg ccgttccgcg catcgaatat acgggacagg agcagtatta cagtttttcc
     2341 ggtcagccga agggcgacga ccttgaaggc ggcgatgatc cgtatcagcc ttcgtattat
     2401 ctcgcgcgca tcaacgtcaa gatgccggac ggctcatccc acgatctgct caaagactac
     2461 gtggcgcgcg tcctcggcac gggcggttat gattacacgg ggatgttcta cgcgacggac
     2521 ggctcgcacg tccgctacga caacaacacg aacacgctct atctacccga cggctcgcgc
     2581 tacatcctcg gcgacccgga cgggtcgaga gaggtttacg gcctcacctc cccgcccact
     2641 tattacatag accgcaacgg caacacgcgc gtctacaacc accagacgaa gcagtggacc
     2701 gatacgctcg gacggtctat cggccaactc aaagacccct ccggcgcggt cgtcggcggg
     2761 atgcccctgc ccgccaaccc gcaggtcggc aactacacat acactctgtc cggcgtgggc
     2821 gggcaggcga tcaattacac gctgcgttgg agcaacttgt cgggtgcgct gaccaacccg
     2881 aatcaaaccc tgcactataa ggggagccac cagtgcggtt cgacctatac agccgtctcc
     2941 ccacaccttt tcagttcgag ctatcctaac tacgtctgct acggcggagt attcaacccc
     3001 gtcgtgctcg ccgagatagc cttgcccgac ggccattctt atcgcttcac ttacaacatc
     3061 tacggcgaga tcgataagat ttatcacccc ggcggcggct atgagcgcgt ccggcacgag
     3121 cagaaaacgc ccctcagtca tatggactcg ccttacagtc aaggcaaccg tggagtggcg
     3181 gaacgttggg tgagcgtgaa gggcgacggc acggacgagg cgcgttggga gtacggcgtc
     3241 agcacggggc agtataactc cgcgccctac gctacctatg tcaacctccc ggacggttcg
     3301 cgcagcgagc gtgtgatgca catgggcagc acgggggggc cggccagccc gcccttcgga
     3361 attgaagacg cgcgcgtcgg catggcctac gaggagcgca gctattccgc gcccgaccag
     3421 tacggcaacc ggcagatgtt gcgccgcacc ctcacggaat gggcgacttc tcccgtcaac
     3481 gtcacggacg agtccggtta cccgatggcc tcggtcacgc gcgccgcgcg ggtggtgaag
     3541 gaggtcgggc tgctgctcga cacgggcggc gcggcgctcg cctccaccgt cacttacagc
     3601 cacgatcaag acctcaacgt caccgcgacg aacaggtaca actacaccac cgtcgccgcg
     3661 agcacggcgc agacggcagc catcggttcg attcccaccg ggacgctcga ccgcacggac
     3721 gaagtgactt atctggtcaa cgacccgtcg gtgagtgcgg gaacgaggca ggcttaccgc
     3781 gaccgcaatc ttctgggtct gccgagttct actcgcgtca agaacggcgc gaccgcgctc
     3841 gtcgctcaga gcgagatcaa atacgacgag gcggcttacc cgctcgtgat ttatggcgca
     3901 gtcaccggct ggactgaacc gggcaccaac gtgcgcggca accagaccac gttgaggagt
     3961 tggatcgaca cttccaacac ctggatcgag acgcatacgc aatacgacca gtgcggcaac
     4021 gcgcgctcca tctgggacgc gaagggcaac cagtcgcaac tctcctacgc agacagtttc
     4081 tccgatggcg tcgggcgcag cacttacgcc tacacgacgc agaccatttc cgccgtcccc
     4141 gacccgtcga acacgcgcgc caccaacacg ccgttagtcg cctcgacggt ctacgactac
     4201 tggaccggca tcaagttgtc tacgacggac gccaacggca aagtcaccac ctaccagtac
     4261 aacgacgcgc tcaaccgtct atccaacgtc aatcatccag acggcgggtg gagcgccttc
     4321 tggtacgggc gcaacgccta cggcgattac atcggcacac gccaggccat caatgcgacc
     4381 caacacacgg agagctacca gttcttcgat ggtctgggca gacccgtccg ttctttcgcg
     4441 ctggacggca gccagtggat cactaccgac acgcagtacg atgctctggg tcgggtgtgg
     4501 agagtctcga atccctacct gtcgggcggg tctggaactc agatcaatcc ttcgggcaac
     4561 tggacgaccc gcagctatga cgcgctcggg cgcgagacga gcctcacgac gccggacggg
     4621 gcggtcacca acaacgccta cagcggcaac cagacgacgg ttacggatca ggccgggaag
     4681 cagcgcagga gcatcaccga cgcgttcggg cgtcaaacgc aggtcatcga agacccgaac
     4741 ggcgtggcct acgtgaccaa ctacacttac gacccgctcg gaaacctgcg ccgcgtggag
     4801 cagggcggcc agtatcgcta cttcatgtac gactcgctcg ggcggctcct gcgcgtgaag
     4861 aatccggagc aagcggcgaa cgcgaacctc gccaccgccg ccgatcccgt ctccggcaac
     4921 acgcaatggt cgctcggcta ctcctacgac aataacggca acgtctccgt caggactgat
     4981 gcccgtgggg tgacgacgac ttatcaatac gacaacatca accggctcaa acagaccaac
     5041 tattcggacg gcacgcccta taccctgcgc acctacgact tcgccacgaa cggtcgtggt
     5101 cgcttctacg ccgattacga atcgagcacg agcggcacgc tcaacttcgt tctcacctat
     5161 gacgcgatgg ggcgtcctac tacgggcaag accgagttct acctgctcgg ctggggctgg
     5221 cgtccggcct acaccatggc gcgcgcctat gacctgatgg ggaacatcac ctcgctcacc
     5281 tatccttcgg gacacacggc gcagtacccg cagcacgacg tgttgggacg actcaagaag
     5341 ttcaacggca acctcggcga cggcgtgcag cgcacctacg cgacgggcat caacccggcc
     5401 aacggcgtcg aagagggcat ccagtacaac gaatccgggc gcatccggca ggagcggttc
     5461 ggcacgcaga cgcccctcta ccacaagcag caatacaatc tgcgcggcca actctccgac
     5521 atccggctcg gcctcggcta cgatgtctgg tctacagagc gcggggcggt agtcatcgac
     5581 tacgggacga cgctcaacaa cggcagcgta gtctcgcagc aatactggat tccgaccgac
     5641 acgacggcca ccgcctggga cgtgcggcag accaactatg cctatgacaa tctgaatcgt
     5701 gtcgccggga tgcaggagtt ttatggtccg gcgggaatgg tcatgcagca gtggttcact
     5761 tacgaccgct ggggcaatcg ttccatcagc ccctcgacca ccgacggcct caacgaactg
     5821 gccttcgacg ttgacgagac gagcaaccgt ctctacgcgc cggggcagaa aaacctcccc
     5881 gactcgtcgc gcaccctgcg ctacgacgcg gcgggcaatg tgacgatgga cgtttacagc
     5941 gcgcacgatg ccctgcgtac tttcgatgcg gaagggcgga tgacggcggc gcacgacagc
     6001 cagagcgcga gagtctatcg ctacacgtat gacgcgggcg ggagacgtat gcggagagac
     6061 ttgtccgggc aggagacgtg gcaggtctat ggcttcgatg gtgagttgtt ggccgagtac
     6121 gcaatcaacg ccgcgcccac gcagccccag aaggaatacg gctaccggaa cggcgagtta
     6181 ctgatcactg ctgaaagcgg cgggcaggtc aactggctcg tcacagacca tctcggctcg
     6241 gcgcgcatga tcgcagacct cagtgggagc ttggcgggca tgcgtcggca cgattatcta
     6301 cccttcggcg aagaggcgtt cgccgggcaa ggtggacgca cgacgggaca aggctatgcg
     6361 agcgacgccg tccgccagaa gttcaccggc tacgagcggg acagtgaaac cgggctggac
     6421 tacgcgcagg cgcgttatca ctctccgacg cagggacgct tcatctcgcc cgacccgctg
     6481 ctgtcgagcg gcgtgcccgc gaacccgcag agttggaatc gctacaccta tgtcctgaat
     6541 aatccgctcg cgctcgttga cccctccggc ctcatctggg gtaagaagga tggcgacgat
     6601 cacatccgct ggttcaacag tgaggcggag atgaaggccg caggttacga ggcgattgat
     6661 aagttctatt acgagcggga cgggacgtgg tatgccgtcg acccgaactc gaaccgctat
     6721 tacaagtcta cggacgggcg aatggcgcaa aaagccttct ggtcattcct cggtctgccg
     6781 gattcgaatt gggacgtgct tccgggtgtg cgttcggtgc ggagtttctt cttctactac
     6841 gcgacgggcg actacaacaa ggcgatgggc agtttcgcgc tcgcatctgt cgagggcggc
     6901 acgagtgcct ccggcgtcaa gagcttgttc aacctcggag cgaaagggat aaagtcaatc
     6961 gtggcgtcgc tcaccgacga cgccgggcgc gcgctggccg acgatgcagt caaacatgca
     7021 aattgcttcg tcgcaggcac tccggttcac accattgagg ggcttaaagc aatagaagag
     7081 attaagccgg gcgatcaagt gttgagttgg aatgagaaga cgcagcgggt tgaatataaa
     7141 acggtcgtgc gcaccttcgc gaaccgcgct tccgcgcttg tggtgctgtc aattgccggg
     7201 gagtcaacgc ccctcaccac cacgccgggt catcccttct acgtgcgtcg tgccagagac
     7261 agcctgaaag tcgccgatga agatgacggc gcgtggatac tggccgtctc gttacgcgcc
     7321 ggagatgagg tgaggcgtcc ggatggtcag tgggtgcgga tagatgacgt tacacaacgc
     7381 gccgagacga cgacggtgta caacttcgag gtcgccgaca accatacata ctacgtcggc
     7441 gacgcgggcg tgctggtgca taatcagtct gttctaagaa ccgtcatcga ggacgatgcc
     7501 gccgccgccg ccgcggataa gatgagggga aatgttgcga gcgcatacga tgagcttaaa
     7561 aatctactct ccagcggacg tccgggaaaa aaccagcatc cattaacggg tgaatacgct
     7621 ggcatgatgg cggcagattt aactggctca ggtaagggta ggggggcgat gcgcgtaata
     7681 ttcagccgta atgataacga agtgataatc cataagatcg tggattatca caagtagaat
     7741 tacaggccac agtttgagtc cgagagtgcc gccagcgcag gggcggtggt gattgggaga
     7801 ggagtcgatg agtacttgga actatcgcgt ggcacgcagg aacggcgagt atggtgtcta
     7861 cgccgcctat tataacgatg acggtacaat cgttagtctc agccgcgacc ctctttctcc
     7921 cacgggcgat agtccggatg aactcagaac gcagttggaa ctgttattgg agtcattgga
     7981 cgcggaggta gttgactatg cctccatcgc ggacaaccgt gaaaagcctg atgacctgta
     8041 acagtcccga taatctctaa ggctcgcctt ccaacctcgc gccgcatgac cttgaggccg
     8101 ccgacataat attgtcggcg gcctgcgcct gctggtgcat catcagttca gttcactatt
     8161 acggttggtg aactcaaccc gctgcattca cctccgactt ccggagcgag gccggagctt
     8221 caggcattat ctgattccga gttattgaat gcggtgacta atccgatcaa aggtgatcct
     8281 atgcagatca acacagtgat aggcaaaata atggatggaa acggtcgtgc ttacgaactt
     8341 ctgcggcacg ctgctgaccc aagtagttca attactccca acacaaggat tccttatgtg
     8401 ccgtatacgc ccatcaaaat cccatgaact tcggtgagca aaaactatga agccaagaga
     8461 agctatacat caggcactct accttgcttt agttgaaata cgcgctgagg catacaccgc
     8521 cgagaacaat aagatttttc atctggccga tctctttcat aacattccgc ttcagttaga
     8581 aagagtttct caggatgaag aaagttatga cgacgtatta gagtggttga ggaagcgcgc
     8641 gagtgagaag ggctttctac agtggctcga ccaagctatt gaatgacaac ctcaggaagc
     8701 gaaaatgagt gaaaacaggt ttggcaagtt agaagctgaa gggctggcat ttctgcacgc
     8761 aggccagttt gctgatgcga tagaagtgtt catgaaactg gccgaagaga atccgggtta
     8821 tgaacatggc atctgcttct atcatctcgc atgttgtttt gaagacgtgg gtgattttga
     8881 aaaagcccgg ctgcattacg agcgtgcgct tcaagctgcc cccggcgatg tgtatagggt
     8941 agggggatac gctgcattct tatatctgca cgggaacccc aaagatgctt ttgaagcaca
     9001 actcaattat ctgcgtgtgg tgcgcaggat ggggaacaag acggatgaag aaaaagcgat
     9061 ctctaatctg aagcatttgg gagcaaaact tggcttgacg gaagacgaaa taatgagaac
     9121 aggtcaatag atttgctccc acaacaaaac gctggagttc tcatgctaaa gagtaacttg
     9181 tgatttttca ggttatccgg cgcaagggtt ccttatcggc tgaacagaat ccgatgccgg
     9241 gcgcgcagcc ggttgaaggt tcgacgccgg ctccccctct ctaccgggac gcagacttat
     9301 ttccgggtcg agcaaggagt ggcacaaaat attttagccg cgatatgcag cgtttgagtc
     9361 gattcaggta tcacaatatt ttgagtcgca tggaaatttt tgccacacta ccctgcgacg
     9421 aaggcgcgtg tcactaatgc attcgggctt aaacttagat cgactatggg cttatctaca
     9481 acattcctga tggcgcttgg ccttgcgatg gacgccttcg ccgtctccgt cgctgacggg
     9541 gcgcgcgccc ggcaacggag gttcagccac gcctttcaac tggcgctcac tttcgggctg
     9601 tttcaagccc tgatgcccct cattggttgg acggtgggac tcggcttcaa agatattatt
     9661 tcgtccgtcg atcactggat cgcgttcggg atgctcggcc ttgtcggcgg taagatgatc
     9721 tacagcgatc tgagcgccaa ggcggcggca gcacccgagt cccgcgtgcc gggaggggtg
     9781 acgacgctct tggtgctggc ggtcgccacc agcatcgacg cgctcgcggt cgggttcagc
     9841 ttaacctttc tcccttccat tctcctgccc gtgctgatcg tcggggtggt tacattctcc
     9901 acctgcctgg cgggcgtgca tctcggccag agatacaggc acttcggcag gagtaaggtt
     9961 caaacggcgg gtggggcgat actgatcgct atcggcatca agattttgat cgagcaccta
    10021 tcctgaggcc gggaatcaat ccaaccgcca atcccaaaga ataaaaaact ctcaggtaac
    10081 tgcgaaatta actaacctct atttggtcga taaggtggcc tggttgagtc cgagtttgac
    10141 agacctctgg gtggcatcgg catcgaaggt cagggtcgtc gaaggcgcgg gcgtccagcg
    10201 caacccgacg tggccgccgt tcccgctcat gagcggcggc gtttctcaga gtcgcactgg
    10261 ccgcgttgcg ggcggcgtgg agcagcggtc agaagtgacg gtcgaggttg cggcgcgcct
    10321 gcccgaagtt gatgtcgggc aggcacgcgt agaatagccc gccgtctgtg acctccacgt
    10381 cgctgtagaa tgcggtcact catcccgcgc cctgcgtact cggcgcgtgc ggtcaatcgc
    10441 acggcttcat cggggaagaa catggagata cctcctccat gtagcggtcg gcggcggcct
    10501 cggcctcctc cgaactgctt ccctgctggc gcgcgctcag gtaggaggca taccaatccc
    10561 accagtgatg ctcggcgtgc gtccgctcgt agcggtcgtg tcgctccgcc gtctcgcgga
    10621 ggagcgctgc aagggttgga acgtccatgg ctcttcctca ctcacgcttc cacgtgcgga
    10681 cgggaagccg cgtcgtgatc tcctgaagca gccagccgtt cccgtccggg tcttcgaacg
    10741 aggcgaacga gaagtaggaa cgcccttccg ggtcgcgccc gccgacgcgc gggttctttc
    10801 cggtgttgtt gaaggggccg ccggcgtagt ggaagacctc gctcacgtca acgccacggg
    10861 cgattaactc gtcgcgggcg gcgtcgaggt cgtctacggc gagcaccatg ccttccaatg
    10921 agccgggctt ggccgacgtg cttcccttgc cgaagatgat cgaggcttcc gagttgtgcg
    10981 gcgtcatctg cacgccacgg aagtcgtcac ccacagtgaa gtctatgtcg agccgccagc
    11041 cgagatttcc gtagaacgcc ttagcgcgat caacgtcgga gacggggatc accaccactt
    11101 cgagcttcat gtctacttgt gcttttttta cttcgatcat tgtgtgttct ccttccaaga
    11161 tatagaactg ccgttcggta tcagggtgcg taatgggtga agacgtagtc gcgctccttg
    11221 atggccgcgt ggcaggcaaa gcaggtgttc atcaaggagt cggcggcggg cttgccgtcg
    11281 tcgaattgag cgtaacccca gccacccgtc gcggcatact ttttcgagtc cttaaccatg
    11341 aactgaaccc cgttcttcgg gtgcccggcc acgaaagatt ggcgacggcc aaagactttg
    11401 ttgttctcct ccgacgggtc gaaactccat gcgagccggg caatgatggt gccttccgga
    11461 aacgggagct tcccttcccg gtaagccttg atcgctacgt cgttgcccag aatggcgcga
    11521 acgtcgtcga gttcgccttc ctcgcgcgcc acggagatga acctccagtc gcggtagccg
    11581 gggggaattg ttttgacgaa gaccgcggcg gcctctttgt cggcgtgtcg ggaggcggtc
    11641 gccttgaagg cgaccacgcc gcccagcgtt gccactgcaa ccagcaagaa gatgatcctt
    11701 ttcataatgc ttcctccttc ggacatctgg taattgaacc cgtgcgcgat cctgcgatca
    11761 cgggtcaggg ttcggaacag gcgctatcac gggcagccct gaaccggagc tgccggtcgc
    11821 ggttggcgga gcgaacgaaa cgcggctcgc aactcttcac tgaagagctg cgggtgctcc
    11881 cacgccgcga agtggccgcc ccggtccgct tcgtggtagt agatcaggtt gcggaaggcg
    11941 cgccgtaccc atgtctccgg cgcacgatag acctcatcgg gaaagaccgt aacggccacc
    12001 gggactgata tctcagtggt cttctgcgcg gtcgcactgg tgacgcttcg tccgccgttc
    12061 tcccagtaca gccgcgcaga ggagatcgcg ctgttcgtca gccagtacag cgtgaagttg
    12121 tccagcacct cgtctttcgt cggagactgt tggggatcgc tgccgtacgt ccactgcgcg
    12181 aaacccgggt gtgggtttcg accctcaaga tttgggcaga ctcttcgaag cgttctacac
    12241 aactaagagc aatggtatgg ggatcggcct ctccgtcagt cgctccatca tcgagagcca
    12301 tcatggccgc ctgtgggctt caccgaacgg cgggacggga gccacgtttt cattctctat
    12361 tactcgcgca tccaggggtg taatggatgc ctccagtatt ggcgcaattc aaacatctgc
    12421 tttgacggat gcggagcggg ctgaaaggca ggcataaaca tgatagtttc aactccgctt
    12481 atatcagtag tcgatgatga cgagtccgtg cgcgagtcgc tacccgatct gctcagagag
    12541 ttcgggttcg cagcccagac gttctcgtcg gcggaagagt tcctcgcgtc cgatttcatc
    12601 ggccagccca agtgtctggt gctcgacatc gccatgccgg gcatgaccgg acccgatctc
    12661 caacaggaat tggcacgtcg gcggcaggag attccgatca tcttcatcac cgctcacgcg
    12721 gacgaaacgg tgcgtccgcg catgatcgaa cgtggtgccg tcgaatgcct gttcaagccg
    12781 ttcagcgaga cggcactgct tgaggcgctc aacgccgcat ttcgcgaatg agccgagatg
    12841 cgccgtgctc gcgagcgcag tgagggctag atatgaatgc agcgaggtta tcacctgagt
    12901 cgcaagataa gccgatcatt ttcgtggttg acgacgacat ctcggtgcgc gagtcgctgg
    12961 agttgctgat cggctttgca ggctggcagc cggaggtctt cgcctccgca gaagagttcc
    13021 tcgcgcaccc gcgagtccac gccccaagct gcctgatact tgacgtttct cttccagacc
    13081 tcaacggtct tgagttgcag aagctcgtcg ccggcgaccg tacggacatg ccgatcatct
    13141 tcatcacggg ttacggcgac gtgccgatga cggtgcaggc catgaaggcg ggagccgtcg
    13201 agtttttgac aaagccattt gctgatgacg tgctgctcgg cgcgatccag cacgccatcg
    13261 agcgaagcga atcttcactc gggaaggagg ccgagacaca ggtgctccgt gatagttacg
    13321 cctcactgac accccgcgaa caagaggtca tggtgctggt cgtttccggc ctgctcaata
    13381 aacagatcgg attcaagctc ggaatcagcg agatcacggt gaaggcgcac cggggcaagt
    13441 tggtgcaaaa gatgaaggcc gactctctcg ccgggttggt gcaaatggcc gtgaaactcc
    13501 gcctcgcacc cgccaggaat ccatggcaga agcaataagc agccggactt tcgaacttcg
    13561 aacaaagagc ggatgttaga tatatcccgc cactggagtc ccaatgcagg ctcgaagaaa
    13621 ccgacgcccc ccagcggaga caccttcaaa cctcacttcg tctaaaacta atctttgcca
    13681 cggaaccgca atttcagcaa cgcaagcgct actggcgacc gagaaggaaa taggtggaca
    13741 tgtcgccctt acctttgacg ttgatcgtac cgcgcggttc aaagaggtag gcatggcgga
    13801 ggcgctcgaa ggttgacgcc gtgacttgaa tgcgtcccgg cgtgctgtgg gattccatgc
    13861 ggctcgctgt gttgacggcg tctccccaca ggtcgtagat gaacttcgac gtgccgatga
    13921 ttccggcgac caccggcccc gtattgatgc cggtgcgaat attaagcgtc gtcccgcgcg
    13981 cgcgattgaa agcggccagc gcagcttgca tgtcgagcgc catttcggcc atagcttcgg
    14041 cgtgatcggc gcggcgttca ggcagccccg ccgcaaccat gtacgcatcg ccgatagtct
    14101 tgatcttttc cagcccgtgc cgcgtggcga gttcatcgaa agacgagaag atttcgttga
    14161 gcagatagac caactcgacc ggcgtaatgc gcgccgacat ctgcgtgaag ccgaccacat
    14221 ccgagaagag gatagtggct tcggtgaaac tttcggcgat gacgccctga gcttctttca
    14281 gacgttctgc gatggccgag ggcaagatgt ttaagaggag gcgttcggac ttctcgcgct
    14341 cgatgcgcaa ctgcgcgagg taagcctgct cccgatcccg caggcgcttc ttctccagcg
    14401 aagcctcgac gcgcgcacgc aggatcaccg gattgaaggg cttgggcaaa tagtcttccg
    14461 cgcccatttc gatgcaacgc acgatgccat cgatctcgtc gagggcggag atcatgatga
    14521 cgggaatgtc tcgcagttcg ggcgtcgtct tgagccgctc cagaacctga tacccgttca
    14581 gaccgggcat cataatgtcc agcagcacga ggtcgaacct gcgcaacccg accgcctcta
    14641 aagcccgcgc gccgtccggg acggtttcaa cttcgtagcc ttcgcgtgtg aggcggcgcg
    14701 cgagaatgtc gcggttggtt tcgctgtcgt ccacgaccag aaccagaccg ccctgctccg
    14761 tctgccgggc gtgcgcgtta tcgatggcat ggatggtgct gacgaggttt gcgatcatcg
    14821 aggagacgcc gtcgtccgcc ggacggaggg ttgcgctatc agcttcgagg tcggagaagc
    14881 ggacgatgtc ttcgatgagt tcgagcaatt ggcgcgcggc ggtctggatt ttttgcaggt
    14941 cggcgacaaa ctcggcatgc tccggcgcgt cggcgtcttc gatcagcatt tcgctgtagc
    15001 cgatcacggc gttgatgggc gtgcgcagtt cgtggcggac gtgcgcgccg aaagagtcga
    15061 ggtcgagatc gggcgtctcg gtgcgcgcgg gattgaggag gtcactgacg cgcgtgcgca
    15121 attgccgccc cgctgtcagg attttttcga ggtcggcgcg aaactcgccc gcgccttcct
    15181 ctgtcgcgct ttcaaccagc atctcgccgt agccgataat ggcgttgatg ggcgaggaca
    15241 attcgtgacg gatgtgcgcc agcagagccg tgcgggtgcg cgccgcatcg gacgtatgat
    15301 catcgcgacc gtgatgggtg tcggcggtca tgaggcggca gaccggttga gcattgtttc
    15361 gatcttttcg agcaggcgcg ggagttcaat cggcttggtg tcgtagtcgt cgcaaccggc
    15421 ttcgagcgcg cgctcgcggt cgccggacat ggcgtgtgca gtcagggcga tgaccgggat
    15481 gtggcgggtc gcgtccgcgt ttttgagttg gcgcgtggcc tcccaaccgt cgagcaccgg
    15541 caagctcatg tccatcagga tgagatcggg cgcgccggat tgagccatct cgacgccctg
    15601 ccgcccgtca acggcgatgc tcacctcgta acctttgcgc accagacgcc gcgagagcat
    15661 gtcgcggttc atctcgttat cttcaaccaa aagtatcttc ttcataatgg ctgtctcgta
    15721 ccctttaaat tttcggatgg aattttatga aggaagttta accaggctgt cgctgccacg
    15781 actgcggacg ctgacctcga ccatctctct gacggcgcgc agcagatcgt cgcggctgta
    15841 agaatccttc tccaaaatct tctcgacata gccgctgagc cggaggcggt cttcgagcgt
    15901 gatgtctttg gcggtgatga cgacgacggg aatcgaacgc gcgccgggca gcttgtgtaa
    15961 ctcctgcaca aactcgaagc cgtccatctc cggcatcatc aaatccagca ggatcaactg
    16021 cggttgttcc tccgccacct gcgcgagggc ggcgcgaccg ttggccgctt cgcgcacctg
    16081 ccagccttcc tgtttgagtc cgcgcaccaa caatgcgcgc gtcgcttcgt cgtcttccac
    16141 gacgagggcg ggggcgttcc ggttcgtcgc ggcgcggcga tatttggcga gcacggcggc
    16201 gagttgctcg cggtcgaagg gcttcgtcat gtagtcggct gcgcccagcg cgtagccttt
    16261 gttcttatcg tctacgatgg tgagcatgat gacggggatg tcggccagtg cggggtcggc
    16321 cttgagggcg gcgagcgtcg cccagccgtc gatgccgggc atcatcacgt cgagcgtgat
    16381 cgcttcgggg cgaagcgtgc gtgcgagttg caacgcctct tcgccgtcgg tcgcgcattc
    16441 gaccctgaaa ccggccttgc cgagagcgcg ctgcaacagg tcgcgtgcca ccggatcatc
    16501 gtccacgacg agcaccatcc cggcgtcttc gtcgaggagt tcagcgcggc cttcgccgcc
    16561 cggcgcgggc gcattagtgt ccggcgactc gctccgcacg tcggcaggca gcttgatgac
    16621 gaacgtggaa ccttcgccgc gcacgctgct gacgcccatc gtgcccccca tcatctcgca
    16681 gaactttttc gtgatgacca gacccagccc cgtgccgccg aactcgcgcg tggtcgaggc
    16741 gtcggcctgc gagaagggct ggaagagctt gttcatctgc tctttcgtga tgccgatgcc
    16801 ggaatctttg acgctgaaat tgatccactc gcgcccgtcc tccctgcttc gcaccacttc
    16861 cagcgtgacg ttgcccgcct tcgtgaactt gcaggcgttc gagagcaggt tgaggagaga
    16921 ctggcgaact ttggtcaggt cggcgtacat cgtgccgagt tcggggtcgg cgtggacgat
    16981 gagcgcgttc tcgttcttgg cggcgagcgg cttgatggtc gattccattt cgccgacgag
    17041 cgcggagagg tcgaatgatt cgaggtagag ttctattttg cccgcttcga tcttcgagag
    17101 gtcgagaata tcgttgatga gggcgagcaa gtggcgcccg gcggtctgaa tcttttggag
    17161 atcggggacg aactcatcct gccctgtgtc ggctgcgtcc tcttgcagca tttcgctgta
    17221 gccgatgatg gcgtttaagg gcgtgcgcaa ctcgtgcgac atgttggcga ggaaggtact
    17281 cttggcctga ttggcggact cggcttcctc tttcgccagt tgcaactcgg cctgcgtctc
    17341 ctgcaattgt cgtagagcgt ctgacagttc atcgttggcg cgcttcatgg cgcgcagcat
    17401 ccgcccgcgc tggatcgtcc tgatcacgac gaccgccagc aggagcgtga cgaagaggat
    17461 cgtgacgagt ttgtagagcg tgttgtcgaa ggagcggttg acggaggtga gttggttttc
    17521 ggcgtcggcc ttgtacagtt gttcgatctg gagcaggtcg tcgcgcacct ggttgaaggc
    17581 cgggatgccg ttcttttgat cagactcggc tgcctctttg atgctgccct ggagaatcag
    17641 cccgatgact tcatcacgat ttttgagata gtgcgcccaa tcgcgctcga acatgtcaat
    17701 ggcatcgagc aactgctgcg acttcgcatg cagccgctct gccgtgatca gttgggcgat
    17761 ccttttcccg gcttcgtggg actgatccgc atacacgatt tgcaggttgg ggtcgtcagt
    17821 cgtcagggcg tagagcatgg tgcggcgagt ctcctgcgtt gagtagagca agtcgccgat
    17881 caggtcaata ccctgcaccg tgccggtgta catctgctgc acctgccgct tcgcgcgcag
    17941 gaggtcgctc acgacgtacc acccgacgcc gacgactatg acgccgatca gcaggaacac
    18001 cagagtgaat ctatacctgt gcaggactcg attcattaac ttaacctatc cgggcgcggg
    18061 gcgtgacgcc cgccacctga gaactcatct gatgcgatag ccgggttggc cgggtttgcg
    18121 cccgtccacg ctcaagacgt tcacctttga attgtccacc tgatccgcgc gcacgtagcc
    18181 gattgcgccg ggcgtgtaat gcacatactg gatcatggcg taagtcgtgt ttaattcttt
    18241 gggcgcttgc tggatggact cgttaaattg tccctgacgg aaatattttt gatagtagtt
    18301 ttcgtcccag ccgtagatgg aacgcaagac cgcgccgcgc tctgactggc cgggaggctg
    18361 gcgcatggcg atgacgactt tcgtgccgtt cggccactgc ccgcgctcgg ccttgaaata
    18421 tttacgcagc acgtcgaacg atatgtcgcg catcgagttt ttcggattaa cgatgatcgc
    18481 cacgtcgccc gtttgcagcg aggcgggcga acgcgtggag gcgaacgcgc gcgccgcctc
    18541 cccgcccgcc gcttcagacc acacggccaa ccggctcgac gccagaagca acgtcaggag
    18601 aacggctagc ccaaagactc tgaccctcat gtaacttccc cttaaaaagt gaacgcgagt
    18661 tgcagaatga gttcatcgag tgtgctcagg ccgcgtcgct ccgtgcgatc atactggctt
    18721 ttgaaggccg cataagtcgt caggtcataa cgcaatccga acgacggccc gttgcgtcgc
    18781 ccgatgtcgc gaaagagaag atcacgcgtc ggcgcattca gatattggta gcgaaaataa
    18841 ggctgcgctt taccgaagcg ccgcgcaaac tgcgtgtaga aaccgggcgt gtaggttgcg
    18901 actccgcccg cgctttccgg cttgtgccgg atgaacacgg cttcgttcag ccattcgtag
    18961 cgtgggcttt gatagacgac gtgcgcggcc atgattcgtt gatcgacgtt tggcgcggcg
    19021 gtcggcgtca ggcggtcgcg gtagaaagag aatccggctt gcagcccagg gagccaatcg
    19081 gggcgcgcgt tgaggccgag gttgtacgac ttgccgttgt tctcatcgaa cgcggtttgc
    19141 accgtgcggt caactgcgga gcgcgcgctc cgcccgttac cgacctcggc tatatagcgc
    19201 aggccgagcg agccggaggg gatgcgcccc gtcgccgaca cgccgacgtt gtgcagcggc
    19261 agcaggccgc cctgaccttc aaaggcgaag agaaaagggc ggttggcggc agtggcgaaa
    19321 taagacccgt gatgaaacgc ggtgttgtaa tagccgatgc cactgtgata acgcccggcg
    19381 ctcaagttca aataatcgtt catcgcatag cgcagcagca ggcggtgaat ctcgaactcg
    19441 aacgaattgt cgcgtttcgc ctccaagatc agttcgctga cgacgctgaa cttttcggac
    19501 agttgcgagg tgatgaacag gtcgagttgt cccagaccga aagcgttcgt cgtgccgcgc
    19561 ccgttgctgg cgcgaaagtt cacgtcggcg aagccctgaa tttggaggcg ggggccggac
    19621 gtgtgggttt cgtgttcctg cccggtgatg gccgcttgcg cctcttcctc ttccggggcg
    19681 gcggcctcgg gcgcagtcgc cgtggtcgcg ggcgcgacgg tcggcggttg gccgttggcg
    19741 gccacggcgt tagccctcag ttccctgacc tgcccttcca gttcctcaac ccgcttaagc
    19801 agttgttgga gttgcgcctg ctcgacggcc tgcgcggcgt gtgcgggagg ctgcctcgcc
    19861 gcgtcctgcg ccgtgaaatt gatttcgaga cggttgaact tctgactcaa gcgcggcgtc
    19921 gtgcccggct gcaactcgaa ggagagcaat gcgtcgcctc ccctctgagc gagacgagcg
    19981 gaggcgaagc cgcgtccctt gaattcatcc ggcatggtcg ccgcaacatc tgccttcggg
    20041 atcaagacgt agaagcgtcc gtcgtcaaaa tatgagtaat ggttgtcgag cgaggcatcg
    20101 gaggcaacta tgacacgcga gccttcgggc gtgtcgctga cgcgcaccgg gacgagtcgt
    20161 ttgcgcggac gggcgacggg cggagtaggc gcgcgatgtg cctttgcgaa gttgtcgtgc
    20221 gcagaggccg aggcggccag acgttccggc tcttgcgcat ggagcgggac ggcgaatgga
    20281 caagcacaga cgaaaaaaag aacgagcgat agaacggacg agacgctagg ggtatgcttc
    20341 atattgaaaa acacttgtgc tggatgcaac gagagcagaa attgcgagcc taccgggtgg
    20401 atacgatgta acagagcaat aatacgacgg ttggccggat gacgccagta cttagatttt
    20461 tttcaatgca gataaaccgc ctaccgtgca acaccccgcc tccttccttt ttagagacta
    20521 aacacgtgat tatgaccact tacacagatt ttatagaaac ttcgacgaaa attgacctta
    20581 agagtctcgc ggaagtagtt ttttccgaac tttaattggc cttctaatag acacaatcaa
    20641 gaatattatt cataaaaatc tcattacctt aaatattgcc aggc
//
LOCUS       DASWNS010000092         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_55713, whole genome shotgun sequence.
ACCESSION   DASWNS010000092 DASWNS010000000
VERSION     DASWNS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55713"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /gene="pstC"
                     /locus_tag="VGB76_03230"
     CDS             <1..588
                     /gene="pstC"
                     /locus_tag="VGB76_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY0097946.1"
                     /translation="IPSVVYGLWGIFVLAPFLREHVSPPLQKLFGWTPFFQGTITGIG
                     LFTGGVILAIMVTPIITAVVRDVLAAVPVTQREAALALGATKWETTRIVLANGAPGIA
                     GAIILGLGRAIGETMAVTMVIGNRPQISASLFDPSYTIASVIANEFTEATGDLYLSAL
                     VELGLVLFLVTFIVNGAARLLVWSVTRSTSGVGRA"
     gene            621..1541
                     /gene="pstA"
                     /locus_tag="VGB76_03235"
     CDS             621..1541
                     /gene="pstA"
                     /locus_tag="VGB76_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY0097947.1"
                     /translation="MFTTHDSSISQSSGSQSSGSRSSGSRSSGNAGASRRRSVVNTVM
                     TTLTALCAFVGVALLLLILGYIAWRGISSISFQFLIDTPKPVGEGGGIGNAILGTLLL
                     LALASAIGLPLGIAAGVYLAEFGRGWFAGAVRFISDTLTGVPSIVTGVFVYTLIVLPM
                     KQFSAMAGGIALAVIMIPIVARTTEEMLRLVPASLREGALALGAPQWRVTTGVVLPAA
                     ASGIATGAMLAVARVSGETAPLLFTAFGSRFFNVYLDQPIASLTVQIFNYAVSPYDEW
                     HAQAWAATLVLMTLILFINVAVRFMTRKKF"
BASE COUNT          279 a          555 c          554 g          350 t
ORIGIN      
        1 atcccttcgg tcgtctacgg gctgtggggc atcttcgtgc tcgcgccatt cctgcgcgaa
       61 cacgtctcgc cgccgctgca aaagttgttc ggctggacgc ccttctttca gggcacgatc
      121 acaggcatcg gacttttcac tggaggcgtt attctcgcca tcatggtgac gccgatcatc
      181 actgcggtcg tgcgcgacgt gctcgccgcc gtccccgtga cgcaacgcga ggccgcgctc
      241 gcgctcggcg cgacgaagtg ggagacgacg cgaatcgtgc tggcgaacgg tgcgccgggg
      301 atcgcgggcg cgatcattct cggtctgggt cgcgccatcg gagagacgat ggccgtgacg
      361 atggtgatcg gcaaccgccc gcagatcagc gcgtcgctgt tcgacccttc gtacaccatt
      421 gcctccgtca tcgccaacga gtttacggag gccacgggcg atctctacct gagcgcgctc
      481 gtcgaactgg gactggttct cttcctcgtc acgttcatcg tcaacggcgc ggcgcgactg
      541 ctggtgtgga gcgtgacgcg ctcgacgagc ggggtcgggc gcgcgtaagg cggcgcggag
      601 atcagagaga cgtgagacag atgtttacaa cgcacgacag ttctatcagt caaagttcag
      661 gcagccaaag ttcgggcagc cggagttcgg gcagccggag ttcgggcaat gcgggcgcga
      721 gcaggcgtcg cagcgtggtc aacacggtga tgacgacgct tactgcgctg tgcgccttcg
      781 tgggcgtcgc actcctgctg ttgattctgg gctacatcgc gtggcgcggc atctcttcca
      841 tcagcttcca gttcctgatc gacacgccga agcctgtggg cgagggcggc ggcatcggca
      901 acgccatcct cggcacgctc ctcctgctcg cgctcgcgtc cgccatcggc ctgccgctcg
      961 gcatcgcggc gggcgtctat ctcgcggagt tcgggagggg ttggtttgcg ggcgcggtgc
     1021 gcttcatctc ggacacgctg accggcgtgc cttcaatcgt cacaggcgtc ttcgtctata
     1081 ccttgatcgt gctgccgatg aagcagtttt cggcgatggc ggggggcatt gcgctcgccg
     1141 tcatcatgat ccccatcgtc gcgcgcacga ccgaagagat gctccggctc gtgcccgcat
     1201 ctctgcgcga aggcgcgctt gcgctcggcg cgccgcagtg gcgcgtcacg acgggcgtgg
     1261 tgctgcccgc cgccgcctcc ggcattgcga cgggcgcgat gctcgccgtg gcgcgcgtgt
     1321 cgggcgagac ggcgccgctc cttttcacgg ccttcggcag ccgcttcttc aacgtctatc
     1381 tcgaccagcc gattgcctct ctaactgtgc agatattcaa ctatgccgtc tcgccctacg
     1441 acgagtggca cgcgcaggca tgggcggcca cgctcgttct gatgacgctc attctgttca
     1501 ttaacgtcgc cgtccgcttc atgacacgta agaagtttta aaagggagcc gctggctgtt
     1561 agctgttagc tgttggcttt cggcagagac aatttggacg tagactgata ccagtcgatt
     1621 tccgatccgg ctcagagcta agagccaatc atttttatga ccaccgcacc gacgccagca
     1681 cgagccatac gacaggaagg cgagcagcct tcgcgcccca agaccacgcc gaccaaga
//
LOCUS       DASWNS010000093         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_314648, whole genome shotgun sequence.
ACCESSION   DASWNS010000093 DASWNS010000000
VERSION     DASWNS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314648"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..980)
                     /locus_tag="VGB76_03240"
     CDS             complement(36..980)
                     /locus_tag="VGB76_03240"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0097948.1"
                     /translation="MVQRKSSGEVPRRLLGRTGVEVSALALGGSHIAETKSKRESLQI
                     VREAVDAGIDFMDNAWEYHEGESEELMGEALAGGLRQKVFLMSKVCTHGRDRKTAMRQ
                     LEQTLRRLRTDYLDLWQVHEVIHDNDPDLHFAKGGVIEALEQAQREGKVRFVGFTGHK
                     DPAIHLKMLAYDYPFDTCQLPLNCFDATFRSFEQQVLPELLRRGIAPLGMKSLGGGGE
                     AVKRRAVTASEALRYAMSLPVAATVSGLDSLKVLRQNLRIARTFTPMSAAEMQDLRTR
                     CAADAADGHFELYKTSKKFDGPPGRKVHKFPSEEELAA"
     gene            complement(1176..1850)
                     /locus_tag="VGB76_03245"
     CDS             complement(1176..1850)
                     /locus_tag="VGB76_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097949.1"
                     /translation="MSDNKNDSIGEEVSAFGQRAKGDVKEATGTVTGDSSLRAEGATE
                     SSTGAARQRSNRMVTGLFRDRDSAERAYGSLSTRGYSKDDVNLLMSDETRDRHFKSDD
                     AADTDLGSKALEGAGAGAAIGGTTGAVLAAIAAIGTSVILPGLGLVVAGPLAAALAGA
                     GAGGATGGLIGALIGSGIPEDRAKAYESGVKEGGIVMGVNARSDEDAEYFENEFRTHR
                     GENIYR"
BASE COUNT          382 a          660 c          644 g          474 t
ORIGIN      
        1 tttcgttcat cgttccgcgt tcctgcttca tcgtttcaag cggcgagttc ctcttcggaa
       61 gggaacttgt gtactttgcg tccgggcggc ccgtcgaatt ttttggaggt cttgtagagt
      121 tcgaagtgtc cgtcagcggc gtctgccgcg cagcgtgtgc gcaggtcttg catctcggcg
      181 gcggacatgg gcgtgaaagt gcgcgcgatg cgtaagttct gccgcaggac tttgagcgag
      241 tcgaggccgg agacggtggc ggcgacgggc agactcatcg catagcgcag agcctcgctc
      301 gcggtcacgg cgcgacgctt gacggcctcg ccgcctccgc ccagactctt catgccgagc
      361 ggggcgatgc cgcgccgtag aagttcgggc aacacctgct gctcgaaact gcgaaaggtg
      421 gcgtcgaaac agttgagcgg cagttggcag gtgtcgaaag gatagtcgta ggcgagcatc
      481 ttgagatgaa tcgcggggtc tttgtgcccg gtgaagccga cgaagcgaac cttgccctcg
      541 cgctgcgcct gttccagagc ttcgatgacg ccgcctttgg cgaagtgtaa atccggatcg
      601 ttatcgtgga tcacttcgtg aacctgccag aggtcgagat aatccgtgcg aaggcgtctg
      661 agcgtctgtt cgagttggcg catggcggtc ttgcggtcgc gcccgtgcgt gcagaccttc
      721 gacatgagaa agactttctg gcgcaagccc ccggccagtg cctcacccat caactcttcg
      781 ctctcccctt cgtgatactc ccatgcgttg tccatgaaat ctatcccggc gtcaaccgct
      841 tcacggacga tctgcaaaga ctcgcgtttc gacttcgttt cggcgatgtg agaaccgccg
      901 agcgcgagcg cggagacttc tacgcccgtg cgcccaagca gcctgcgcgg cacttctcca
      961 gaagatttgc gctgaaccat gacgacccct ccttgatgct ttcccgacgt ggccggaagc
     1021 aacctgaaat ttaattgttg aacgatgaga cggagtatgc cacaacaaaa ggcgcgtcga
     1081 gaagatttaa gttgagcgac ggggaggcaa aatggagacg ggagactcaa atattttgag
     1141 cctcccgtct ttcacttcgt taacatcccg cgtctttacc ggtagatgtt ctcgccgcga
     1201 tgcgtgcgaa actcgttctc gaagtattcg gcatcttcgt ccgagcgcgc gttgacgccc
     1261 atcacgatgc cgccttcttt gacgccgctc tcatacgcct tcgcacggtc ttcggggatg
     1321 cccgaaccga tgagcgcgcc gatgagtccg cccgtcgcgc cgcccgcgcc cgcgcccgcc
     1381 agtgccgccg ccagcggtcc ggccacgacc aaccctaagc ccggcaagat cacggacgtg
     1441 ccgatggctg cgatagccgc gagcactgcg cccgtcgtgc cgccgatggc cgcgcccgcg
     1501 cccgcgcctt ccagagcttt gctgccgagg tctgtgtcag ccgcgtcgtc ggatttgaaa
     1561 tggcggtcgc gcgtctcgtc cgacatcaag aggttgacat cgtctttgct gtagccgcgc
     1621 gtcgagagcg aaccgtaggc gcgctccgcg ctgtcgcggt cgcggaacag tcccgtcacc
     1681 atgcgattcg acctctggcg cgccgcgccc gtcgaactct ccgtcgcgcc ttccgcgcgc
     1741 agcgaactgt cgcccgtcac cgtgcccgtc gcctccttga cgtcgccttt cgcgcgctgg
     1801 ccgaaagctg aaacctcttc gccgatgctg tcatttttat tgtcgctcat aatcttccct
     1861 ttcactgtga tggataagga ctactgcggc ggttctcgcc cggagtcctt taaatgtttg
     1921 ccactaaccg tcccgcttga tatttgagag agtagactcg gcagccgtta ttcgttgcga
     1981 cagatgcgcg ccgcgccgtt cgtttacaag accgcgtaca gtctttgact ctggcgcgat
     2041 tttgtccgtg tacgattttg cgtcttaaaa attgctgccg gggtggagat ggcaaagcgc
     2101 gtgccgttga ttctaaaagg gcgtgaagcg tgaagcgtaa atcgtaggaa aagccgtaaa
//
LOCUS       DASWNS010000094         9098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_29614, whole genome shotgun sequence.
ACCESSION   DASWNS010000094 DASWNS010000000
VERSION     DASWNS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29614"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VGB76_03250"
     CDS             complement(<1..719)
                     /locus_tag="VGB76_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY0097950.1"
                     /translation="MPETATPEIVNVTEEIKRKAGRLLFIGLPGTRLDKRLREMLREV
                     RPGGVILFGRNIETAEQVALLNAQIRDALGGRVIIGVDQEGGLVDRFRDICEPMPSAK
                     AVRLAGRSELARKFGELSARALRLLGFNMNFAPVLDLKGGNEENGLRGRTFGETPEEV
                     SRLAGAYLDGLQAHRVVGCGKHFPGLGGSQVDSHRRLPVVTHSWEEILQHDLVPFLDL
                     MQHRRGEQLHSVMVSHAAFPEV"
     gene            868..1839
                     /gene="queG"
                     /locus_tag="VGB76_03255"
     CDS             868..1839
                     /gene="queG"
                     /locus_tag="VGB76_03255"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY0097951.1"
                     /translation="MVAEELSQLAPPRFTARLKERALELGFDKVGIVAAAPLDAERAR
                     LEEWLRRGYHGEMNWMAREPERRTDPRLVMPGARSVVVVALNYFTPHTHDADPAQGKI
                     SRYAWGDDYHDVVGGKLKELLAWIKEECPEAEGKTCVDIQPLMDKAWAARAGLGWIGK
                     HTNLITREYGSWVFLGELLLNLELEYDAVASEDHCGTCTLCIESCPTEAITEAYVVDS
                     NRCISYATIELRTPEIPEAVAPHLEGWLYGCDICQDVCPWNRFEQPTTETRFEPREGE
                     VAASLSDLLALTPDAYAARFRRSAIKRAKLIGLQRNARALQKNRDED"
     gene            2063..3382
                     /locus_tag="VGB76_03260"
     CDS             2063..3382
                     /locus_tag="VGB76_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097952.1"
                     /translation="MPSLRRLSAQLSARRVPLVLTFVFVAAAFAFLCIEPPASSACPV
                     VLPKTMRMLYNESALVAVVRVADSVEVKKEEDMTLKKIGLRISSLLKGESKGRVVDLY
                     LQFWGDLDGSSANVFQKGEVLLVFLDANEDGEGYALTDFERGAKKLSPDDLKVYVSRL
                     EELAAIMRSKKPDAAALTEWLVRCAEEPATRWEGAYELAPDGLPFEDSGEPGDADADA
                     DVANAEVSVTLDDPGREQAKPEGVAAAQAATTTNGVVPANDADESGERADPQEIMRRL
                     RAAAGEPQVDFAALLTPAQKERLMTVLLAAEEWNEGEQMLLLLAANWKDARLVPLSLK
                     HLARMADKPTYQAEDLMRVVAHMLADRTLIKFVADYSEEASYADTGISDEEHSAEETE
                     AGGATAEERAASKREMEEMKAAAAEARFKRSGKLRLFLALAEQPQKP"
     gene            3587..4588
                     /gene="moaA"
                     /locus_tag="VGB76_03265"
     CDS             3587..4588
                     /gene="moaA"
                     /locus_tag="VGB76_03265"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY0097953.1"
                     /translation="MTTAVLKDSYGRAIRDLRVSLTDRCNFRCFYCLPHGEPPIAPKE
                     QMLSYEEIEYLSEIFVSLGIEKIRLTGGEPMMRRDIETIIGKLAALKPRLHDLALTTN
                     GYFLPGRAAALKEAGLDRITISLDSLRPDTFRRMTGVDVLPRVLEGIEAARAARLTPI
                     KINAVIVRGHNDAEVADFAAFARAHDIAMRFIEFMPLDSGHEWSREMVVSGREMRAAI
                     EERFPLAPLPVRRGAETSARYRFADGAPGEIGIIAPVTEPFCGACSRIRLTADGQIRT
                     CLFSTVEHSLRDVLRSGATRGQIVEYIESVVMKKEPRHYINDPDFLQPARTMSFIGG"
     gene            4610..5158
                     /locus_tag="VGB76_03270"
     CDS             4610..5158
                     /locus_tag="VGB76_03270"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0097954.1"
                     /translation="MSTKTEATIEDLYKVEGKAELVNGEIVHMSPTGSQPGYAGDEIF
                     VSLREYCKRTKTGRAVGDNKAFRVDLPNRKSFSPDAAFHVGADTGMKFYEGAPIFAVA
                     VRSEGDYGAKAECAMARKHPEYFAAGTEVVWDVDLLSEDVVRVYRASDPDNAQIYRRG
                     ERAEAEAAVPGWSMAVGDLFVE"
     regulatory      5203..5321
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5394..7367
                     /gene="thiC"
                     /locus_tag="VGB76_03275"
     CDS             5394..7367
                     /gene="thiC"
                     /locus_tag="VGB76_03275"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY0097955.1"
                     /translation="MSTTEEIDATPEQQSERQSDEVQLPNSGRVYIEGAREGVRVPFR
                     EVTLNPTRNPDGTLEENPCVRVYDTSGAWGDPEIKCDERTGLTPLRRDWIIARGDVEE
                     YQGREVKPEDDGYLTAGAAEYAQQKRKGRLEYFPGLRRAPLRARAGARVTQMHYARRG
                     HITPEMEFVALRENMGREMALHALTSHSRDDRSSLFHEHRGESFGASIPEFVTPEFVR
                     DEVARGRAIIPANVNHPESEPMIIGRNFLVKINANIGNSAVASSIEEEVEKMRWSTKW
                     GADTVMDLSTGKNIHATREWILRNSPVPIGTVPIYQALEKVGGRAEELTWEIYRDTLV
                     EQAEQGVDYFTIHAGVRLPYIPLTAKRTTGIVSRGGSIMAKWCLAHHEESFLYTRFRE
                     ICEIMAAYDVSFSLGDGLRPGCIADANDEAQFAELETLGELTQIAWEYDCQTMIEGPG
                     HVPMHLIKENMDKQLATCGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLC
                     YVTPKEHLGLPDKKDVKEGVIAYKLAAHAADLAKGHPGAQLRDNALSKARFEFRWEDQ
                     FNLSLDPEVAREYHDETLPQEGAKLAHFCSMCGPHFCSMKITQEVRDFAARKELEESA
                     ALAAGMKEKAAEFVATGAEIYQGERPAGAHHDH"
     gene            7557..8963
                     /locus_tag="VGB76_03280"
     CDS             7557..8963
                     /locus_tag="VGB76_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097956.1"
                     /translation="MPRSTVLDAYERAGFVIIARIVSVEKTEKAAPQGQMSNGENYVD
                     GVKSTRMMIERVFKGSLKAGDEITFGQGGGADCVWTFREKEIGEQFLFYLGGREKNQL
                     LWYAGTCGRSGGLKYVGDDLLYLNKLDRVRGKSRLSGTLRFADNDELSVEGRRIRITG
                     AGKTHELKTDADGVYEIYDLPAGQYLVEPEIPPGWKFDSYYHRAPTGAAAGSGSDAAK
                     KLPVTVEAKKHTALNLVFEIDNAIRGKVYDPRGRPLDNVCLNLVPVEGDHSNYFYKAD
                     CTEGGGMFEIREIPSGSYLLVVNKAGKISSSEPFKTFYHPDVFERERATVISIGAGET
                     LEGLDIRAPHVEETITVEGVFLYSDGKPVVGESVEFVPGQTGENVEGEARAQTDAQGR
                     FSFKILKGLEGELYGEMYVYASKFENCPKLEAIIKKAPQGSTDVKTPVMKIHALNDLS
                     GVELRYPFPGCKKAKTPE"
BASE COUNT         1886 a         2766 c         2811 g         1635 t
ORIGIN      
        1 acctcaggaa aggccgcgtg gctcaccatc accgaatgca gttgctcgcc gcgccggtgc
       61 tgcatgagat cgagaaaggg cacgaggtcg tgttgcagta tctcttccca cgaatgcgtg
      121 acgacgggga gacgccggtg cgagtcaacc tgtgaaccgc cgaggccggg gaaatgcttg
      181 ccgcaaccga cgacgcggtg tgcctgcaag ccgtcgagat atgcgcccgc gagcctcgac
      241 acttcttccg gcgtctcgcc gaaggtgcgc ccgcgcagtc cgttctcttc gttgccgccc
      301 ttgaggtcga gcacgggcgc gaagttcatg ttgaagccga ggaggcgcag ggcgcgtgcg
      361 gagagttcgc cgaacttgcg cgcgagttcg ctgcgtccgg cgaggcgcac ggctttggct
      421 gaaggcatcg gttcgcagat gtcgcgaaag cgatccacga gtccgccctc ctgatccacg
      481 ccgatgatga cgcgcccgcc gagcgcgtcg cgaatctgcg cgttcagcag cgcgacctgc
      541 tccgccgtct cgatgttgcg cccgaagagg atgacgccgc cggggcgcac ctcgcgcaac
      601 atctcgcgca ggcgtttatc gagccgcgtt cccggcaagc cgataaacag gagtcgtccg
      661 gccttgcgct tgatctcttc cgtcacgttc acaatctctg gcgtcgcagt ttcgggcatc
      721 gttcgattac ggttttcctt ttcctccagc ttaaagggtc gaagggctga cgcgcaagaa
      781 ggcagccgcc gccttccttc atacacaacc attaacgctc gtcttccgct gaaccggagg
      841 cggacgcttt gctatacttc tccccgcatg gtcgcagaag agctatcaca actcgcgccg
      901 ccgcgattca ccgcgcgcct caaggagcgc gcgcttgagt tgggcttcga caaggtgggc
      961 atcgtcgccg ctgcgccgct cgacgccgag cgtgcgcgcc tcgaagagtg gctacggcgc
     1021 ggctatcacg gcgagatgaa ttggatggcg cgcgagccgg agcggcgcac agacccgcgc
     1081 ctcgtgatgc ccggtgcgcg ctccgtcgtc gtcgtcgcgc tcaactactt cacgccgcac
     1141 acgcacgacg ccgacccggc gcaaggtaaa atctcccgct acgcctgggg cgacgattat
     1201 cacgatgtcg tcggcggcaa actcaaagaa ctcctcgcgt ggatcaagga ggaatgcccg
     1261 gaggccgaag gcaagacttg cgtggacatt caaccgttga tggacaaggc ttgggcggcg
     1321 cgcgcggggc ttggctggat cggcaagcac acgaacctga tcacgcgcga atacggctcg
     1381 tgggtcttcc tcggcgaact cctgctcaac ctcgaactcg aatacgacgc cgttgcctcg
     1441 gaagatcatt gcggcacttg cacgctctgc attgaatcat gcccgacgga agcgatcacc
     1501 gaagcttacg tcgtggattc aaaccgctgc atctcctacg ccaccatcga actacgcacg
     1561 cccgaaattc ccgaagcggt cgccccgcat ctcgaaggct ggctctacgg ctgcgacatc
     1621 tgccaggatg tctgtccgtg gaaccgcttc gagcagccga ccacggagac gcgcttcgag
     1681 ccgcgcgaag gcgaggtcgc cgccagtctc tccgatctgc tcgcgctcac gcccgacgcc
     1741 tacgccgcgc gctttcgccg cagcgcgatc aaacgcgcca agctcatcgg actccaacgc
     1801 aacgcacgcg cgctccagaa aaatcgggac gaagattgac cgtccgcata accgggacga
     1861 cgatccaaaa aaatattttt ccgtaggaca actctcagcg cgccagccct cttctcatgt
     1921 gcagaccaac cgagtgggca gttcttcatt gggaactttt gtcgcgcgcc tgcgtctaag
     1981 agttcgctgg cttcataaaa ttatcgtgtc gggtcgggta ggcaatttta cctcgcagat
     2041 aaatgccgaa aggggtacgt ctatgccaag tctccgtcgc ctgtcagctc aactctccgc
     2101 gcgccgcgtg ccgctcgtct taactttcgt ctttgtcgcc gccgccttcg cgttcctctg
     2161 catcgaaccg cccgcgtctt cggcctgtcc cgtcgtcctg ccgaaaacga tgcgcatgct
     2221 ctacaacgag agcgcgctcg tcgcagtagt gcgcgttgcg gattccgtcg aggtcaaaaa
     2281 agaagaagac atgacgttga agaagatcgg gctgcgcatc tcttcgctgt tgaaaggaga
     2341 gagcaagggg cgtgtcgtcg atctctatct acagttctgg ggagacttgg acgggagttc
     2401 cgcaaacgtt tttcagaaag gcgaggtgct gctggttttt ctcgatgcta acgaggatgg
     2461 agagggctac gcgctcacgg acttcgaacg gggcgcaaag aagttgtcgc cggacgatct
     2521 gaaagtctac gtcagccgtc tcgaagaact cgccgcgatc atgcgcagca aaaagccgga
     2581 cgcggcggct ctcaccgaat ggctcgtgcg ctgcgccgaa gaacccgcga cgcgttggga
     2641 gggcgcatac gaactcgctc ccgatggcct gccgtttgaa gactccgggg aacccggcga
     2701 cgcggatgcc gacgccgacg tggcgaacgc ggaggtcagc gtgacgctcg acgaccccgg
     2761 acgggaacag gcaaagccgg aaggcgttgc tgccgcacag gccgcaacga cgacgaacgg
     2821 cgtggttccc gcgaacgatg ccgacgaatc cggcgagcgc gccgacccgc aggagatcat
     2881 gcgccgcctc cgcgccgcag cgggagagcc gcaggttgac ttcgccgcgc tgctcacgcc
     2941 cgcgcagaaa gagcgtctga tgacagtgct gctggccgcc gaagagtgga acgagggtga
     3001 gcagatgctt ctgttgctcg ccgccaactg gaaggatgcg cgcctcgtcc ccttaagcct
     3061 caagcatctc gcgcgcatgg cggacaagcc tacctatcag gccgaagacc tgatgcgcgt
     3121 cgtcgcccac atgctcgccg accggacgct catcaagttt gtcgcagact acagcgagga
     3181 agcgtcttat gcagacaccg gcatcagcga cgaagagcat agcgcagagg agacggaagc
     3241 tggcggcgcg accgccgaag aacgcgccgc gtccaaaaga gagatggaag agatgaaggc
     3301 cgccgccgcc gaagcgcgct tcaaacgcag cggcaaactg cgcctcttcc tcgctctggc
     3361 cgagcaaccg caaaaaccct gacacggcct ccgaagttcc tcaccggcgg gacgcgtccc
     3421 atactcttcc gcgcgtcccg ctgctttcga atttttcgcg gacgctcttc tccgtcgcct
     3481 tgcgcaacgc ctcgcgcctg gacttggtac gctccgtcgc gcggcgtatc atgttagtga
     3541 aacttcgtcg ctaaagttac aggcgttgcc cggtgaaaga ttagaagtga ctactgctgt
     3601 gctcaaagat tcttacggtc gggcgatacg cgacctgcgt gtgtcgctga cggacaggtg
     3661 caactttcgt tgtttctact gcctgccgca cggcgagccg ccgattgcgc ccaaagagca
     3721 gatgctctct tacgaagaga tcgaatacct ctcggagata ttcgtctcgc tcggcatcga
     3781 aaaaattcga ctcaccggcg gcgagccgat gatgcgccgc gacatcgaaa cgatcatcgg
     3841 caaactcgcc gcgctcaaac cacgcctgca cgatctcgcg ctcacgacga atggctactt
     3901 cctgcccgga cgcgccgccg cgctcaaaga ggcgggactc gaccgcatca ccatcagtct
     3961 cgacagcctc cgccccgata cgtttcgccg gatgacaggc gtggacgtgc tcccgcgcgt
     4021 gctcgaaggc atcgaggcgg cgcgcgccgc gcgcctcacg ccgatcaaga tcaacgccgt
     4081 catcgtgcgc gggcacaacg acgcggaggt ggcggacttc gccgcgttcg cgcgcgcgca
     4141 cgacatcgcg atgcgcttca tcgagttcat gcctctggat tccggccatg aatggtcgcg
     4201 ggagatggtc gtgtcgggca gagagatgcg ggcggcgatt gaagagcgtt ttccgctcgc
     4261 gccgctgccc gtccggcgcg gcgcggagac ttcggcgcgc taccgtttcg ccgacggcgc
     4321 gcccggcgag atcggcatca tcgcgcccgt cacagaaccc ttctgcggcg cgtgctcacg
     4381 catccgcctc accgccgacg gccagatcag aacctgcctc ttctccaccg tcgaacactc
     4441 actgcgcgac gtgctgcgga gcggcgcgac gcgcggccag atcgtcgagt acatcgaatc
     4501 ggtggtcatg aaaaaagagc cgcgccacta catcaacgac ccggactttc tccagcccgc
     4561 acgcacgatg agcttcatcg gcggctaacc tgaaacgagg tgcgcccgca tgagtacaaa
     4621 gaccgaagcg accatcgaag acctctacaa agtcgagggc aaggcggaac tcgtcaacgg
     4681 agagatcgtc cacatgtcac caaccggcag ccagcccggt tatgcgggcg acgaaatttt
     4741 tgtgtcgttg cgcgaatact gcaaacgcac taaaactgga cgcgccgttg gcgacaacaa
     4801 agccttccgg gttgatttgc ccaatcgaaa atcctttagt cctgatgcag cctttcatgt
     4861 cggcgcagat acgggcatga aattttatga gggcgcgccc atcttcgccg tcgcagtgag
     4921 aagcgagggc gactacgggg cgaaggccga gtgtgcgatg gcacgaaaac accccgagta
     4981 tttcgcggcg ggcactgagg tcgtctggga tgtcgatctg ttgagcgagg atgtggttcg
     5041 cgtctatcgc gcgagcgatc ctgacaacgc gcaaatctat cgtcgcggcg agagggctga
     5101 ggctgaggcc gccgtgccgg gttggtcgat ggcggttggc gacctgtttg tggaatgaca
     5161 cgcgcgtaag tcataatccg ccgttcgttt ttgacaatcg agaaaagttt tcgggggagc
     5221 ttcgcccaac cagagcgggc gcggctgaga gtcacaaatg tgtgagactc ctagaacctg
     5281 atgcggttaa taccgccgaa gggagaaaca ccggaagacc gcttgcgacc cttcatgtgt
     5341 ccgggcggtc tttcttttca ggccggactt gcggctgaat ggatgacaac caaatgagta
     5401 caaccgagga aattgacgcg acgccggagc agcagtccga acgtcagagc gatgaagtgc
     5461 aactgccgaa ctccgggcgc gtctacatcg aaggcgcgcg cgagggcgtg cgcgtgcctt
     5521 ttcgtgaagt cactttgaac ccgacgcgca accccgacgg cacgctcgaa gagaacccct
     5581 gcgtgcgcgt ctacgacacg agcggtgcgt ggggcgaccc tgagatcaag tgcgacgaac
     5641 gcaccggact cacgccgctg cggcgcgact ggatcatcgc gcgcggcgac gttgaagaat
     5701 atcagggacg tgaagtgaag ccggaagacg acggctatct gacggcgggc gcggcggagt
     5761 acgcgcaaca aaagaggaaa ggacggctgg aatatttccc cggcctgagg cgcgccccct
     5821 tgcgcgcccg tgcgggggca cgcgtcacgc agatgcacta cgcgcggcgc ggccatatca
     5881 cgccggagat ggagttcgtc gcgctgcgcg aaaacatggg gcgcgagatg gccttgcacg
     5941 cgctcacctc gcactcacgc gacgaccgca gttcgctctt tcacgaacac cgtggcgaat
     6001 ctttcggcgc gtcaattccg gagttcgtga cgcctgaatt cgtgcgcgat gaagtggcgc
     6061 gcgggcgcgc gatcattccg gcgaacgtca accatcctga gtctgagccg atgatcatcg
     6121 ggcgcaattt cctcgtcaag atcaacgcca acatcggcaa ctctgcggtc gcctcttcga
     6181 ttgaagaaga ggtggagaag atgcgctggt ctacgaagtg gggcgcggac acggtgatgg
     6241 acttgtccac gggcaagaac attcacgcca cgcgcgagtg gattctgcgc aactcgcccg
     6301 tgcccatcgg gacggttccg atctatcagg cgttggagaa agtcggaggc cgcgccgaag
     6361 agttgacgtg ggagatttac cgcgacactc tcgtcgaaca ggccgaacag ggcgttgact
     6421 acttcaccat ccacgcgggc gtgcgcctgc cttacatccc gctcacggcg aaacgcacga
     6481 cgggcattgt ctcgcgcggc ggctcgatca tggcgaagtg gtgcctcgca catcacgagg
     6541 agagctttct ctacacgcgc tttcgagaga tctgcgagat catggcggcc tacgacgttt
     6601 cgttctctct gggcgacggg ctccgccccg gctgcatcgc agacgccaac gacgaagcgc
     6661 agtttgccga actcgaaacg ctcggcgaac tcacgcagat cgcctgggag tacgactgcc
     6721 agacgatgat cgaaggaccc ggccacgtcc ccatgcacct catcaaagag aacatggaca
     6781 agcaacttgc gacctgcggc gaagcaccct tctacacgct ggggccgctc accaccgaca
     6841 tcgcgcccgg ctacgatcac atcacctccg ccatcggcgc ggcgatgatc ggctggtacg
     6901 gcacggccat gctctgctac gtcacgccga aagagcatct gggcttgccc gacaagaaag
     6961 acgtgaaaga gggcgtcatc gcttacaagc tcgcggcgca cgcagccgat ctggcgaagg
     7021 gtcatcccgg cgcgcaactg cgcgacaacg cgctctcgaa agcgcgcttc gagtttcgct
     7081 gggaagatca attcaacctc tcgctcgacc cggaagtggc gcgcgaatat cacgacgaga
     7141 cgctgccgca ggaaggagca aagctcgcgc acttctgctc gatgtgcgga ccacacttct
     7201 gctcgatgaa gatcacgcag gaggtgcgcg acttcgcggc cagaaaggaa cttgaagagt
     7261 cggctgcgct cgcggcggga atgaaagaga aggcggcgga gtttgtcgcc acgggcgcgg
     7321 agatttatca gggcgagcgt ccggcgggcg cgcatcacga tcactgagcg cacgggtaag
     7381 cgacgggtga gttgacgccc cgcaacggcg acggcagcgt tgcggggcag acggaaggac
     7441 gagccatatt tcgcaagaaa ggacgagcca tgtttcgcaa aagttgcttt tcgtttctgt
     7501 tgctgatctt cgctcttcta ttctccggcg cgactgacgc gcatgcctgt tcctgcatgc
     7561 cgcgttccac ggtgctcgat gcttacgagc gcgccgggtt cgttatcatt gcccgaatcg
     7621 tgtcggtcga aaaaacggag aaggccgccc cacagggtca gatgtccaac ggcgagaatt
     7681 atgtggacgg ggtgaagtca accagaatga tgatcgagcg ggtcttcaaa gggagtttga
     7741 aggccggtga cgagataact ttcgggcagg gcggcggcgc agactgcgtc tggactttca
     7801 gagagaaaga gatcggcgag cagtttctgt tttatctcgg cgggcgcgag aagaaccaac
     7861 tcctctggta cgcgggcact tgcggcaggt cgggcggctt gaaatatgtg ggcgacgact
     7921 tgctgtatct gaacaagctc gaccgcgtgc gcggcaagtc gcgtctgtcg ggcacgctca
     7981 ggtttgctga caacgatgaa ttgagcgtcg agggcaggcg gattcgcatt accggggcgg
     8041 gcaaaactca cgaactcaaa acggacgcgg acggggttta cgaaatctac gatctgcccg
     8101 ccggtcaata tctggtcgaa cctgaaatac cgcccggctg gaagtttgat agctattacc
     8161 atcgcgctcc gaccggagct gccgccggga gtgggagcga cgcggcgaaa aagctccccg
     8221 tcaccgtcga agccaaaaag cacaccgcac tcaaccttgt tttcgagatc gacaatgcca
     8281 ttcgcggcaa ggtttatgat ccgcgcgggc ggcctttgga taatgtctgc ctgaacctcg
     8341 tgcccgtcga aggcgaccat tcaaactatt tctacaaggc cgactgcacg gagggaggcg
     8401 ggatgttcga gatcagagag attccgtccg ggagttatct gctggtcgtc aacaaagcgg
     8461 ggaagatcag cagcagcgaa ccgttcaaga cgttctacca tccggacgtg ttcgagcgcg
     8521 agcgcgcgac cgtcatcagc atcggcgcgg gggagacgct cgaaggtctg gacatccgcg
     8581 cgccccacgt ggaagagacg atcacggtcg aaggcgtctt cctttactcg gacggaaagc
     8641 ccgtggttgg agagagcgtg gaatttgtac cggggcagac cggagagaac gtggaaggcg
     8701 aagcccgtgc gcaaaccgac gcgcagggac gcttctcgtt caaaatcctg aagggcttgg
     8761 agggcgagtt gtacggcgag atgtacgtct atgccagcaa gtttgagaac tgtccgaaac
     8821 ttgaggccat catcaaaaaa gccccgcaag gttcgacgga tgttaaaaca cccgtcatga
     8881 aaattcatgc cttgaacgat ctctccggcg tggagttgag ataccctttc cccggctgca
     8941 agaaagcgaa gacgccggag taaaagtacc ccaccgcaag cggtggggct ttagagtgat
     9001 ttagtcgaac aggtctattt gcgtatcttc ggtttctttg tgacgttgga tgtatttacg
     9061 aatcacttcc gccgtcactt tgtcgcccac agttctgg
//
LOCUS       DASWNS010000095         4641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_29620, whole genome shotgun sequence.
ACCESSION   DASWNS010000095 DASWNS010000000
VERSION     DASWNS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29620"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..800)
                     /gene="xth"
                     /locus_tag="VGB76_03285"
     CDS             complement(33..800)
                     /gene="xth"
                     /locus_tag="VGB76_03285"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0097957.1"
                     /translation="MKIATWNVNSVTARLPVVLQWLRRARPDVLCMQELKCTDERFPR
                     EAFAELGYRAEVYGQRTYNGVGIVSRSPITDVERGFPDDEEGSHARLLAATVEGVRLI
                     NVYVPNGQSVGTDKYRFKLDWLKRLREFIDDEYWTDDEVLLCGDFNVAPEDRDVHDPQ
                     LWRGRVLFSKPEKDALAHVKEWGFTDAFRQHEPGSGHYSWWDYRAGSFRQNTGVRIDH
                     IWVSENLAARCTNAWIDKEPRGWERPSDHTPVVAEFK"
     gene            978..1640
                     /locus_tag="VGB76_03290"
     CDS             978..1640
                     /locus_tag="VGB76_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY0097958.1"
                     /translation="MAAAAFYDLEGTLVSTNLVHTLGFYARNQQGVLRSFRKTATTVL
                     SIPLFAATDQYSRKVFNDLFFKRYKGETEDRLRFFADELFETVLKPAVFPGTFELIEK
                     SRSLGLRQIVVTGALDFSVEPLMKYLGIETYVSNRLEFVNGIATGRLLPPVLAAATKA
                     SWIRNYAEQENINLSESYAYTDSMSDLPMLSIVGHPAAVNPDMRLRQTALHHDWPILN
                     LK"
     gene            1719..3317
                     /locus_tag="VGB76_03295"
     CDS             1719..3317
                     /locus_tag="VGB76_03295"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEY0097959.1"
                     /translation="MALQLRKKLHESVVYIDRDSAPRIVASGMDFILEDLPVGTRVIY
                     PNQPLKGLVNREAAIRYALNHPHDCDPLFAQLTPGMKVTIAVDDISMPLPPMNTPDIR
                     QTALEILVEMLNGHGVDDIHIIIAISLHRKMTPAEMKRMAGAKIWDDFYPDRYYNHDA
                     EDPSNLVLLGETRHKEPLRINRRAAESDLLIYLNFNFVPMNGGHKSVGVGLCDYESLR
                     AHHNPNTIRASNSYMDPAGSELGRVISRVGKLADEHLNVFHIETTINNHMFGAEMGFL
                     TKNEDDFSALDRMKYEAMRFTMSKMPRPAKRAMYNSLKGNYEMIACHAGRTDPVHERI
                     IAKSMEQYVVPIEGQCDILICPVPDMSPYSVNSSLNPLLVQVMALGYHFNMYRGKPLL
                     KKGGTLILHHPCTDEFDHNFHPSYIEFFNRLLPESRDANVLRDKYELEFANNPSYVEM
                     YRRGNAYHGSHPFFMWYWGENGRQHVGHVIAAGAENAHVPAMMGWERADNLTEAIAMA
                     RSYQGRSAEITMLHQPVIAICDME"
     gene            3518..3724
                     /locus_tag="VGB76_03300"
     CDS             3518..3724
                     /locus_tag="VGB76_03300"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY0097960.1"
                     /translation="MRCAVIIEKGESSYGADVPDLPGCVAAAETKAEVEELIREAIKF
                     HIEGLQAEGLPVPEPSSYFEYVEA"
     gene            3733..>4641
                     /locus_tag="VGB76_03305"
     CDS             3733..>4641
                     /locus_tag="VGB76_03305"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0097961.1"
                     /translation="MKLSPTEIFQGRKIFLIGSTGFLGKVTLSMLLYRFPGIERVYLT
                     VRSRSLAESETRFWNNVITAPPFDPLRERFGDAFEDFIRDKVRVYNGDIGDTNLGLTE
                     DEAKAIAADIDVVINSAGNVTFNPTLESALRTNVVGTQNVIAFAKRMRRPALVHISTC
                     FVAGNRSGPVWESDPVIGYFPRREELPGVEFSVEQEIRDCAKLADRVKDEAKDAMQVA
                     RFREGARKRLIEEGRDADDLDAMGLAVARERKVWTRTRLTDLGIERAEWWGWPNIYTY
                     TKSLGEQLVAAETGIVRSIVRPSIVES"
BASE COUNT         1003 a         1458 c         1293 g          887 t
ORIGIN      
        1 gctttataat ccgcgttcag cattcactta attcacttaa actccgccac gaccggcgtg
       61 tgatccgaag gccgctccca gccgcgcggc tctttgtcga tccatgcgtt cgtgcaacgc
      121 gcggcgagat tttccgacac ccagatgtga tcgatgcgga cgcccgtgtt ctggcgaaac
      181 gacccggcgc gataatccca ccaagaataa tggccgctgc ccggctcatg ctgccggaac
      241 gcatccgtga agccccattc tttaacgtgg gctaaggcat ccttctccgg cttgctgaac
      301 aggacgcgcc cgcgccacag ttgcgggtcg tgcacgtccc tgtcttcagg cgcgacgttg
      361 aagtcgccgc agagcaatac ttcgtcgtcc gtccagtatt cgtcgtcgat gaattcccgc
      421 agccgtttca accagtccaa cttgaaacga tacttgtccg tgccgaccga ttgaccgttc
      481 ggcacataga cgttgatgag acggacgcct tcgaccgtcg ccgcgagcag gcgcgcgtgc
      541 gagccttctt cgtcgtctgg aaaaccgcgc tcgacatccg tgatgggcga gcgcgagacg
      601 atcccgacgc cgttataagt tcgctgcccg tacacttcgg cgcggtagcc gagttcggcg
      661 aaagcctcgc gcgggaaacg ctcgtccgtg catttcaact cctgcatgca caaaacgtcc
      721 gggcgcgcac gccggagcca ttgcagcacg acgggcaggc gcgccgttac agagttcaca
      781 ttccaggttg cgattttcac gttgactcat attatatcgg cgcgccttaa aattcccact
      841 gccgaagaca ttgctacata aaaccatcga aatagagtat atttttcttc ctgcgcagtt
      901 cagccctaaa ttttgcagac agacgccgcg ccaacccgcg cttttattgc cgccgggcgc
      961 gtcgttagga ggaaagtttg gcagccgccg ctttttacga tctcgaaggc actctggtca
     1021 gcaccaatct cgtgcacacg ctcggtttct acgcgcgcaa tcaacagggc gtgctgcgtt
     1081 cgtttcgcaa gaccgccacg accgttctga gcatcccgct cttcgccgcg accgaccagt
     1141 acagtcgcaa ggtcttcaac gatctcttct tcaaacgcta caagggcgag acggaagacc
     1201 gcctgcgctt cttcgctgac gaactcttcg agacagtcct gaagcccgcc gtcttccccg
     1261 gcaccttcga gttgatcgaa aaatcgcgca gcctcggcct gcggcaaatc gtcgtcacgg
     1321 gcgcgctcga tttctccgtc gagcctctga tgaaatatct cggcatcgaa acctacgtct
     1381 ccaaccggct cgaattcgtc aacggcatcg ccaccgggcg actcctgccg cccgtactcg
     1441 ccgccgccac gaaggcgagt tggatcagaa actacgccga acaggagaac atcaacctga
     1501 gcgagtctta cgcctacacg gacagcatga gcgatctccc gatgctctcc atcgtcggcc
     1561 acccggcggc ggtgaacccc gacatgcgac tgcgccaaac cgcgctgcac cacgactggc
     1621 cgatacttaa tttgaagtga cgagtggcga gtgacaagtg acaagttaaa acttgttctt
     1681 gctcgtcact cgtcactcgt cacccgtcac ttaaaaatat ggctctgcaa ctcagaaaaa
     1741 aactgcacga atcggtcgtc tatatcgacc gcgacagcgc gccgcgtatc gtggcttcgg
     1801 ggatggactt catcctcgaa gatttgcccg tcggcacgcg cgtcatctat ccgaaccagc
     1861 cgctcaaggg tctggtcaac cgcgaggcgg cgatccgtta cgcgctcaat cacccgcacg
     1921 attgcgaccc gctctttgcg caactgacgc cgggcatgaa ggtgaccatc gccgtcgacg
     1981 acatttcgat gccgctcccg ccgatgaaca cgcccgacat ccgccagacc gcgctcgaaa
     2041 ttctcgtcga gatgctgaac ggccacggcg tagacgacat tcatatcatc atcgccatct
     2101 cgctccatcg caagatgacg cccgccgaga tgaagcgcat ggcgggggcg aaaatctggg
     2161 acgactttta tccggatcgc tactacaatc acgacgccga agacccctcg aatctcgtcc
     2221 tcctcggcga gacgcgccac aaagaacccc tgcgcatcaa ccgtcgcgcc gccgagagcg
     2281 acctgctcat ctatctcaac ttcaacttcg tgccgatgaa cggcggccac aagtcggtcg
     2341 gggtcggcct ctgcgattac gagtcgctgc gcgcgcatca caacccgaac acgatccgcg
     2401 cgtcgaacag ttacatggac cccgccgggt cggagttggg gcgcgtcatc agccgcgtcg
     2461 gcaaactcgc ggacgagcac ctgaacgtct ttcacatcga gacgaccatc aacaaccaca
     2521 tgttcggcgc ggagatggga ttcctcacga agaacgagga cgacttctcc gctttagacc
     2581 gcatgaagta tgaagccatg cgcttcacga tgagcaagat gccgcgcccc gccaagcgcg
     2641 ccatgtacaa ctcgctcaag ggcaactacg agatgatcgc ctgccacgcc gggcgcacag
     2701 accccgtcca cgaacgcatc atcgccaaga gcatggagca atacgtcgtg cccatcgaag
     2761 gccagtgcga cattttgatc tgccccgtgc ccgacatgtc gccctacagc gtcaactctt
     2821 cgctgaaccc tctgctcgtg caggtgatgg cgctcggtta tcatttcaac atgtatcgcg
     2881 gcaagcccct gctcaaaaaa ggcggcacgc tgattctgca ccatccctgc acggacgaat
     2941 tcgaccacaa ctttcatccg agctacatcg aatttttcaa ccgcctgctg cccgaatcgc
     3001 gcgacgcgaa tgttttgcgc gacaagtacg aactggagtt tgcgaacaat ccgagctacg
     3061 tcgagatgta ccggcgcggc aacgcctatc acggctcgca ccccttcttc atgtggtact
     3121 ggggcgagaa cggacgccag cacgtcggcc acgtgatcgc cgccggagcg gagaacgcac
     3181 acgtccccgc catgatgggt tgggagcgcg ccgacaatct caccgaagcg attgcgatgg
     3241 cgcgcagcta tcaaggccgc tccgccgaga tcacgatgct ccaccaaccc gtgatcgcga
     3301 tttgcgatat ggagtgagtt tgtttgaaag tccgggatgt cttcaagcgt ttggaggacg
     3361 acggagggca gttagtgcgc atgaaaggca gccatcgcta gtttcgtcac ccgacgaaga
     3421 ccagattagt gacggttgca ggcaagcccg gagttgacgt gccaacggga acgttaaaga
     3481 atatctggcg tcaggctgaa attgaggaaa atgtttgatg agatgcgcgg tcatcatcga
     3541 aaagggcgaa tcgagctacg gcgcagatgt gcctgatctt ccgggctgtg tcgccgccgc
     3601 cgaaactaaa gcggaagttg aagagttaat tcgagaagcc atcaagtttc atattgaagg
     3661 tctgcaagcg gaaggattgc ccgtgccgga gccgtcgagt tatttcgagt acgtcgaagc
     3721 gtgaagcaga gcatgaagtt atcaccgaca gaaatttttc agggacgcaa aatcttcctc
     3781 atcgggagca cgggctttct gggcaaggtg acgctgagca tgttgctcta tcgcttcccc
     3841 ggcatcgagc gcgtctatct caccgtgcgc tcgcgctcgc tcgccgaatc cgagacacgc
     3901 ttctggaaca acgtcatcac cgcgccgccc ttcgacccct tgcgcgaacg cttcggcgac
     3961 gccttcgagg atttcatacg cgacaaggtg cgcgtctata acggcgacat tggcgacacc
     4021 aatctcggct tgacggaaga tgaagcgaaa gcgattgccg ccgacataga tgtcgtcatc
     4081 aacagcgcgg gcaacgtcac gttcaacccg acgctggaga gcgcgctgcg cacgaacgtc
     4141 gtcggcacgc agaacgtcat cgcgttcgcc aagcgcatgc ggcggcctgc gctcgtgcat
     4201 atctccacct gcttcgtcgc gggcaaccgc tcgggtccag tgtgggagag cgatccggtg
     4261 atcggatatt ttccgcgccg cgaagagttg cccggcgtcg agttctcggt cgagcaggag
     4321 atacgcgact gcgcgaaact cgccgaccgc gtgaaggacg aagcgaaaga cgcgatgcag
     4381 gtggcgcgct tccgcgaagg ggcgcgcaag cggctcatcg aagaggggcg cgacgcagac
     4441 gatctcgacg cgatggggct ggccgtggcg cgcgagcgca aggtgtggac gcgcacgcgt
     4501 ctcacagacc tcggcatcga acgcgccgaa tggtggggct ggccgaacat ctacacttac
     4561 acgaagagtc tgggcgaaca actcgtcgcg gcggagacgg ggatcgtgcg ctccatcgtg
     4621 cgcccttcaa tcgtcgaatc g
//
LOCUS       DASWNS010000096         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_341131, whole genome shotgun sequence.
ACCESSION   DASWNS010000096 DASWNS010000000
VERSION     DASWNS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341131"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /gene="nhaA"
                     /locus_tag="VGB76_03310"
     CDS             <1..932
                     /gene="nhaA"
                     /locus_tag="VGB76_03310"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY0097962.1"
                     /translation="LPIFAAAGGICVPALIHFGLNAGTPTQAGIGIPMATDIAFALGV
                     LALLGSRVPASLKVFLTALAVMDDLGAIIVIALFYTAELSVAYLIGALAVFGLLVAIN
                     RLLRVMSLIPYLLGGALMWFLMLKSGVHATIAGVLLAFAIPFSAKEDDAESPSHKLEH
                     LLHMPVAFIILPIFALANTGIVVGTGWAQNLTGANSTGIIAGLVLGKPVGITLLSFVA
                     VSIGICRLPLDLKWRHVFGAGLLGGIGFTMSIFITNLAFVGQAETINASKMAILLASL
                     TAGTIGFLWLKLLGQPAPSDVDMDTMDFAEAEA"
     gene            1080..1448
                     /locus_tag="VGB76_03315"
     CDS             1080..1448
                     /locus_tag="VGB76_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0097963.1"
                     /translation="MLRRGLFITLALVVGLAGCAQVRRAERSPETDALLQAARAGHAD
                     TVKSLLAGGKADVNGRDEHGNTPLIEAARAGHDDVVQALLIATADVSAKNDEGQTALS
                     VAARGGHNEVIRLLRQAGAS"
     gene            complement(1647..1913)
                     /locus_tag="VGB76_03320"
     CDS             complement(1647..1913)
                     /locus_tag="VGB76_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097964.1"
                     /translation="MSSNQSDNSQGDTGTRDISYDLLSISYHLLQGAETLALYIADAR
                     QEGNDELVQFFQETKDEFTRRAERSKALLVQYVGQEQAQAASGS"
BASE COUNT          497 a          763 c          833 g          602 t
ORIGIN      
        1 tactccccat cttcgcggcg gcgggcggca tctgcgtgcc cgcgttgatt catttcggtt
       61 tgaatgcggg gacgcccacg caggccggga tcggcatccc gatggctacc gacatcgctt
      121 tcgctctcgg cgtgctcgcg cttctcggca gtcgcgtccc ggcctcgctg aaagtatttc
      181 taaccgcgct cgccgtgatg gacgacctcg gcgcgatcat cgtcatcgcc ctgttctaca
      241 cggcggaact ctcggtcgca tacttaatcg gcgcgctcgc ggtgttcgga ttactcgtcg
      301 ccatcaatcg tctgttacgt gtgatgagtt taatcccgta tcttctcggc ggggcgttga
      361 tgtggttcct gatgctcaag tccggcgtcc acgcgacgat tgccggggtc ttgctggcgt
      421 tcgccattcc gttctccgct aaagaagacg acgcggaatc gccttcgcat aaactcgaac
      481 atttgctgca catgcccgtc gcctttatca tcctgcccat cttcgcgctg gcgaatacgg
      541 gtatcgtcgt cggcaccgga tgggcgcaaa acctaacggg cgcgaacagc acgggcatca
      601 tcgccggtct ggtcttaggc aagccggtag ggatcacgct gttgagtttc gtagcggttt
      661 ccatcggcat ttgccgcctg ccgctcgatt tgaagtggcg acacgttttc ggcgcggggc
      721 tgctcggcgg catcggtttc acgatgtcga ttttcatcac gaatctggcg ttcgtcgggc
      781 aggcggagac gatcaacgcc tcgaagatgg cgatcctgct ggcttctctg acagcgggga
      841 caatcggttt cctgtggctc aaacttctcg gccagcccgc gccgtcggat gtggatatgg
      901 acacgatgga tttcgcggag gcggaagcat aacttacccg ttcagtttga gatttggttc
      961 tgcgcagtcg ttgagttgaa ggatgaggcg ctatccgggc gagggcggga agatgataag
     1021 ttcaaccgtt gacagactgt ttcgcgcctg tcgaaggcgc tggaagaagg agattgctga
     1081 tgcttagacg tggactgttc atcacgctcg cgctggtcgt cgggctggca ggttgtgcgc
     1141 aggtgcgacg cgcggagcgt agccccgaaa cggacgccct gctgcaagcg gcacgtgccg
     1201 ggcacgcgga cacagtgaaa agccttctgg caggcgggaa ggcagacgtt aacggcaggg
     1261 acgaacacgg caacacgccg ctcatcgagg ccgcacgcgc gggtcatgac gacgtggtgc
     1321 aggcgctcct gatcgcaacg gctgatgtga gcgcaaagaa cgacgaaggg cagacggcgc
     1381 tctctgtagc agctcggggc gggcacaacg aagtcatccg cctgctcagg caggccgggg
     1441 cgtcgtgacg gtcgcaggac agccgacgaa cggcggcggg tggctttcgc gcgagcgggc
     1501 actgtccctc gtgttttgcg tcgcgatata atctctgcaa cgcaacgtaa gaagaggcca
     1561 gccgcgatgt ggctggcctc ttcttcttct acttgtcttg ggctgagcac ccggttaacg
     1621 ctgaactcgc ccgtcagcca accccgttag gaaccggatg cggcctgcgc ctgctcttga
     1681 ccgacatact ggacgagcaa cgccttggat cgctcggcgc gtctggtgaa ctcgtccttc
     1741 gtctcctgaa agaattgcac cagctcatcg ttgccttcct gccgggcgtc tgcgatgtag
     1801 agggcgagcg tttccgcccc ctgtagcagg tggtaagaga tgctgagtag atcgtagctg
     1861 atgtcacgtg tgccggtgtc gccctggctg ttgtcgctct gattgcttga catgatttcc
     1921 ctcctggtaa agtcgtgttg atgtagtggg ggcttcaggt gaagccctca ttgtagagag
     1981 gcgctttgca ggggtcaagg taggagcagg cctgaggctg tcagacctgc gctctccgtc
     2041 gcgctccgac tgtcggcggc cagaacgcca aaaccctctg ccgttagcgg ccttgccgca
     2101 ggtcgttcct ccggagagat cgtcgctcgc cgggtatcaa ctcccatttg ccgcgccgat
     2161 tccaagcacg acaggctctc gatggccgtg cctcgcagcg tgccgtcggt ggccgtgttg
     2221 caaaaaatat tttggccgca ataggatgtg ctgaagcgta tgtggccttc tcaatatctt
     2281 gtatagtcgg aaagtttttg caacacttct ccctgtgcat tgcgaaaagg tagattgacc
     2341 ccccgataga ggtcgaaatg gccgatgctt acagccatcg gtcgaggccg gataatgaat
     2401 cattcacctg cccgcctctt agagcattgc gcgctctgcg ctataagggc ataaagaggc
     2461 cattacttaa ggctcatcgc tttctcatca tattactcgg aacgaagatc aagcggaggt
     2521 tgagcagtgc gtctggtcac ggtcacagcc ccgaaaggga aaggcgtcga agtagccggg
     2581 tttgctctcg acgcaggtat ctcccgcgct tcaatccatg aggtgcagat ctatactcac
     2641 aacggtgata tagagataga ggatgtggtt gagatcagtt cttcgacccc gcagg
//
LOCUS       DASWNS010000097         4961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_497259, whole genome shotgun sequence.
ACCESSION   DASWNS010000097 DASWNS010000000
VERSION     DASWNS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497259"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..894)
                     /locus_tag="VGB76_03325"
     CDS             complement(4..894)
                     /locus_tag="VGB76_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097965.1"
                     /translation="MVTKQKKRELDFSDFPPGTVNAYTRLVCLACIFDLFTKQLGLAP
                     RTAYSEIKRHAPTAAELTALEPARPYFDSAEKHPRCPYCDAAKRWHARLDTFRIESGK
                     ATDAPRRALVKSLPKPEEQFALIETRSTKRDIFFAWLDALGRQLDFTDDAWLIRTTHA
                     MLERKEPKTDWAEIFSRVRAVRRSQRLAEGWEQEGARLYLSPPLYTDALLIQYLVSRS
                     QAHGGITFEGRLTLVELVRRLRYSGQLEAQGITERDQFEVLEQLIEKLTGGNTPAKLY
                     FLVDRRDFLDKVKTVYSHYV"
     gene            1347..3236
                     /locus_tag="VGB76_03330"
     CDS             1347..3236
                     /locus_tag="VGB76_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0097966.1"
                     /translation="MIRKLSPYRRRLQAIAFFSLLVIFAAVFVSLSAAFSVSNAGRSL
                     TASQRVTRIREIQGAAHLSPLNGASVSGVDGIITANRANGFFMQDPAPDNDPRTSEGI
                     FVLAPVRASAVVGDRVRVDGTVTEARPGANAETNANLSVTQINSSSVEVLSSGNALPA
                     PVVIGAGGRRPPLNVIEDDARGNVESGGTFDPASDGLDFYESLEGMRVQVRDAVAVAR
                     SFALRSGAREITVVGDGGRVARLRTPRGGIVIRPDDFNPERIFLSGELSALPDLNVGD
                     RFGGTLEGVLDYNFGNYKLIVTRAPRVARGGLRRETTTAARAGQLSVATFNVHNLDPN
                     DSAEKFRQLADIIVKNLKSPDLLAIEEIQDNNGATNDAVVDAAETFARLISAIKLAGG
                     GVYSFRSINPVDDQDGGEPGGNIRIGFLFRTERGLRFIDRPGGSPTASTTVVNEANVP
                     RLSSSPGRIEPANPAFNQTRKSLAGEFTFNGHKLFVVANHLSSKGGDHPLFGRFQPPV
                     RQSETKRHQQAQIVNGFVNSILAVDRNANVIVLGDLNDFEFSTTLQRLAGDALKNLSE
                     TLPQGERYTYIFEGNSQALDHILVSRRLFASLVRFDVVHINAEFFEQASDHDPTVAVF
                     RLPAR"
     gene            3381..4556
                     /locus_tag="VGB76_03335"
     CDS             3381..4556
                     /locus_tag="VGB76_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097967.1"
                     /translation="MPLIALKLKSERRRLMLVATLALVLTASASMLAAPAFAQSIEPN
                     SPTPVLSNEIAGRIAPLDIGDPRLTRFFYAFNGLQGDLELTVESYNLDGDIDIFLAGN
                     LRPLTKVTLYAGATATKSSKTVYLRRDEPLILRVQARTPNDAEGTYRVTFGGAFKPAE
                     RLASDVASSGETTDANPSPARTPAASANRNVRRVTSTGARVEEPVAEVAVREEPVREA
                     ETREAPPVAAKKSAPRPRPDRSRAGARGNTRTQNARSGSPGAGTGSGGETEKADAPKP
                     DDNKTDMGAEERPAPPERPRPVRVPRGRNNRNTGTARRTGAPPAEVVPTAPAETPAPP
                     APSPRLVLVMRNGERFERDMSGVRRVTVENGMIVIVSKNGKTERQPMAAVLRMSIEP"
     gene            4686..>4961
                     /locus_tag="VGB76_03340"
     CDS             4686..>4961
                     /locus_tag="VGB76_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097968.1"
                     /translation="MAERQNPDGTRRASKRRVAGRIAVASLAVVVALLLYSRGGMWAS
                     PEQWQAGGAGFVTAFVIVLIITASLACGLTASYFLILTPLLFPLHWSA"
BASE COUNT          955 a         1557 c         1528 g          921 t
ORIGIN      
        1 ctgtcagacg tagtgggaat agacggtctt aactttgtcg aggaagtcgc ggcggtcaac
       61 tagaaaatag agtttcgcgg gcgtgttgcc gccggttagt ttctcgatca actgttccag
      121 cacttcaaac tggtcgcgct cggtgatgcc ctgcgcttcg agttggccgg agtagcgcag
      181 gcggcgtacg agttcgacaa gcgtgagcct gccttcgaag gtgatgccgc cgtgagcttg
      241 cgaacggctg acgagatatt gaatgaggag cgcgtccgtg tagagcgggg gcgaaagata
      301 gagccgcgcg ccctcctgct cccaaccttc tgcgagccgc tgcgaacggc gcacggcgcg
      361 cacgcgtgag aaaatttccg cccaatccgt cttcggctct ttccgctcca gcatggcgtg
      421 cgtcgtgcgg atgagccacg cgtcgtctgt gaaatcgagt tggcgtccga gcgcgtcgag
      481 ccacgcgaag aagatgtcgc gtttggtgga gcgcgtctcg atcagtgcaa actgctcttc
      541 cggtttcggg agcgatttga cgagcgcgcg gcgcggcgca tcggtcgctt tgccgctttc
      601 gatgcggaag gtgtcgagcc gcgcgtgcca acgcttcgcg gcgtcgcaat aggggcagcg
      661 cgggtgcttc tctgcgctgt cgaagtaagg gcgcgcgggt tcgagcgcgg tcaactctgc
      721 ggcggtcggc gcgtggcgtt tgatctcgga ataggccgtg cgcggagcga ggccgagttg
      781 cttggtgaag agatcgaaga tgcaggcgag acagacgagc ctcgtatatg cgttgaccgt
      841 gccgggcgga aaatcgctga aatccagttc gcgctttttc tgttttgtta ccatgaactc
      901 attttacagg agcgacggag caatcttgta taacccccgt gaatggcaat tagaattgac
      961 aaccaattga cttgagcata aggtctgcac gctatcgccc tgcttgctcc tgcgcttctc
     1021 aactgtccgg gaggcttccc gtaactttca tcttccaaag gccgacatcg tgatgccaag
     1081 attaatcgca ctccttctcg cctgctcaac cctgctcgcg ccttccgcgt cgtttcaatt
     1141 ccgccctgtt tgcgcgtccc gccagcttga atttcaaaaa agtcatcttc aataccgagg
     1201 cgcatcccgt ctggaagacg tggtgagttg agcgtcccac tcgtctccgg cgcgcgcgtc
     1261 tctttagtcg cccggcgaga accggcgtgc gccttgccct ctccggcgac atcgcgcaca
     1321 attaaccgct gtagcccata ccaactatga tcagaaaact ttcgccctac cggcgacgcc
     1381 tgcaagccat cgccttcttt agtctccttg tcatcttcgc tgccgtcttc gtctcattga
     1441 gcgcagcgtt cagcgtctca aacgcggggc gaagccttac ggcctctcag cgcgttacac
     1501 ggattcgtga gattcagggc gcggcgcacc tctcgcctct gaacggcgcg tccgtgtccg
     1561 gcgttgacgg aatcatcacc gccaaccgcg cgaacggctt tttcatgcaa gaccccgccc
     1621 ccgataacga cccgcgcacc tccgaaggaa tattcgtgct cgctcccgtt cgggcgagcg
     1681 cggttgtcgg cgaccgcgtg cgcgttgacg ggacggtgac agaggcgcga ccgggcgcaa
     1741 acgcagaaac caacgcgaac ctctccgtca cacagatcaa ctcttcttcc gtcgaagttc
     1801 tctcaagcgg gaatgcgctg cccgcgcccg tcgtcatcgg cgcgggtggc cgacgcccgc
     1861 ccctgaatgt catcgaagat gatgcgcggg gaaacgtgga aagcggcggc acgttcgatc
     1921 cagcgtctga cgggctggac ttttacgaaa gcctcgaagg gatgcgcgtg caggtcaggg
     1981 atgcggtggc cgtggcacgc agtttcgctt tgcggagcgg cgcgcgcgag attaccgtcg
     2041 tgggcgacgg cgggcgcgtc gcacgcctgc gcaccccgcg cggcggcatc gtcattcgac
     2101 ccgacgactt caaccccgaa cgcatcttcc tctccggcga gttgagcgcg ctacccgatc
     2161 tcaacgtggg agatcgtttc ggcggcacgc tcgaaggagt gctcgattac aatttcggca
     2221 actacaaatt gatcgtcaca cgcgcgcccc gcgtggcacg cggcggatta aggcgcgaga
     2281 cgacgacggc tgccagagcg gggcaactct cggttgcgac cttcaacgtc cataacctcg
     2341 acccgaacga cagcgcggag aagtttcggc agttggccga catcatcgtc aaaaatctca
     2401 agtcgcccga cctcctcgca atcgaagaga tacaggataa caacggcgcg acaaacgatg
     2461 cggtcgtaga cgccgccgaa actttcgcac gtttgatctc agccatcaaa ctcgcgggcg
     2521 gcggggtcta ttccttccgc agcatcaacc ccgtggatga tcaggacggc ggcgaaccgg
     2581 gcggcaacat ccgcatcggt ttcctcttcc gcacggagcg cggcctgcgt ttcatagatc
     2641 gccccggcgg aagtccgacc gcttcaacta cggtagtcaa cgaagcgaac gtcccgcgcc
     2701 tgtcgtccag ccccggacgc atcgagcctg cgaaccccgc tttcaatcaa acacgcaagt
     2761 cgctcgcggg cgaatttact ttcaacgggc acaaactttt cgtcgtcgcc aaccacctct
     2821 cgtccaaagg cggcgaccac ccgttgttcg gacgctttca accgcccgtg cgccagagcg
     2881 aaacgaagcg acaccagcaa gcgcagatcg tcaacgggtt cgtcaactcg attctggcgg
     2941 ttgaccgcaa cgcgaacgtg atcgtcttag gcgaccttaa cgacttcgag ttttccacca
     3001 cgttgcagag gctcgcgggc gacgccctga aaaatctgtc cgagacgctg ccgcagggcg
     3061 agcgttacac gtacatcttc gaaggcaact cgcaggcgct cgatcacatt ctcgtctcgc
     3121 ggcgtctctt cgcctcgctc gtcaggttcg acgtggtaca catcaatgcg gagttcttcg
     3181 agcaggcgag cgatcatgat ccgaccgtcg cagtcttccg gctgcccgcg cgctagttaa
     3241 atgagtcgtc gaagagaggc ggcggcttga tcccgtcgcc ttttttgttt gcccgcaagc
     3301 ggcggcgcgt aggctttgtt cgtaaacgtg tagactgtta aactctccgc agttcaagtt
     3361 tacccggcga ccgcaagctg atgcctttaa ttgctctcaa actaaaatcc gaaagacggc
     3421 gtttgatgct cgtggcgacg ttggccttgg tgctcacggc gtcggcctcg atgctcgccg
     3481 cgcccgcctt cgcacagtca atcgaaccga attctccgac gcccgtactc tcaaacgaga
     3541 tcgccgggcg catcgccccg ctcgacatcg gcgacccgcg cctgacgcgt ttcttctacg
     3601 ccttcaacgg cctgcaaggc gatctcgaac tcacggtcga aagttataac ctcgacggcg
     3661 atatagacat cttcctcgca gggaatctgc gcccgctgac gaaggtgacg ctttacgccg
     3721 gggcgactgc cacgaaaagc tcgaagaccg tctacctgcg ccgcgacgaa ccgctcatcc
     3781 tgcgcgtgca ggcgcgcacg ccgaacgatg cggaagggac ttatcgcgtc acgttcggcg
     3841 gcgcattcaa acccgccgaa cggctggcga gcgacgtcgc ttcgtccggc gagacgacag
     3901 acgcgaatcc gtctccggcg cgtacgcccg ccgcgagcgc gaacaggaac gtaaggcgcg
     3961 tcacctcgac gggagcgcgc gtcgaagagc cggtcgcaga ggtcgccgtc agagaagaac
     4021 ctgtcaggga ggcggagacg cgcgaggctc ctccggtcgc ggcgaaaaag agtgcgccca
     4081 gacccagacc ggacagaagc agagcgggcg cgcggggcaa cacacgcacg cagaatgcgc
     4141 gcagcggttc gcccggcgcg ggaacgggca gcggcggcga gacggagaaa gccgacgcgc
     4201 cgaagcccga cgataataag actgacatgg gcgcggaaga gcgacccgcg ccgccggaac
     4261 gcccgcgccc cgtcagagtg ccgcgcgggc gcaacaacag gaacacgggc acggcgagac
     4321 ggacgggcgc accgcccgcc gaagtcgtcc cgaccgctcc ggcagagact cccgcgccgc
     4381 ccgcacccag cccgcgcctc gtcctcgtca tgcgcaacgg cgagaggttt gagcgcgaca
     4441 tgtcgggcgt gcgccgcgtg acggtcgaga acggcatgat cgtgatcgtc tcgaagaacg
     4501 ggaagacaga gcggcaaccg atggccgccg tcttgcgcat gtcaatcgaa ccctgacgaa
     4561 gcgttgagac tgtgaagcgt tgggaaaggc ggagagcggg gaagagttga gcgttgaaaa
     4621 aggcgggcgt tgcgacaggc gcacggttga aaaagttctc gacgagcaga ctcttgagcg
     4681 agccaatggc agagcgacaa aatcctgacg ggacgcgtcg agcgtcgaag cggcgcgttg
     4741 cgggacgcat cgccgtggcg tcgctggcgg tcgtggttgc gctgttgctt tatagtcgcg
     4801 gcggcatgtg ggcttcgccg gagcaatggc aggcgggcgg cgcgggattc gtcaccgcct
     4861 tcgtcatcgt cctgatcatc accgcgagtt tggcgtgtgg cttgacggcc tcctattttt
     4921 tgatcctcac gccgctcctg ttcccgcttc actggtcggc t
//
LOCUS       DASWNS010000098         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_29809, whole genome shotgun sequence.
ACCESSION   DASWNS010000098 DASWNS010000000
VERSION     DASWNS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29809"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1049
                     /gene="sucD"
                     /locus_tag="VGB76_03345"
     CDS             165..1049
                     /gene="sucD"
                     /locus_tag="VGB76_03345"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY0097969.1"
                     /translation="MSVLVDKNTRLVVQGITGKEGSFHTKQMVEYGTNVVGGVTPGKG
                     GTTHEGIPVFNTVEEAVREAGANVSIIYVPPPFAADAIIEAAAAGMPLVVCITEGIPA
                     IDMVGVKRFIEGTGTRLIGPNCPGIISPGKCKIGIMPGHIHREGNIGVVSRSGTLTYE
                     AVGQLTALGLGQSTAIGIGGDPIIGTTHTDALALFEADPETEGIVMIGEIGGTAEEEA
                     AAYAKAHVTKPIVAFIAGQTAPPGRRMGHAGAIIAGGKGTAAEKMSALEAAGIRVVRS
                     PADIGTAMRDALGASASA"
     gene            1098..1808
                     /locus_tag="VGB76_03350"
     CDS             1098..1808
                     /locus_tag="VGB76_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097970.1"
                     /translation="MKRYGLRLVILVALAAQASGVGMPFAGAQQNWEKAPFQNWGAAD
                     VKAVLEKSPWSRVIEQNTVIAPSLLGVNAPKMEEKTIILLRSALPVRQALLRERQLAS
                     KYESMNAADRAAFDAKNKALLDCPACARYYVISIKCNYACYGGSFVEDHKKSFYLLND
                     KGERRELAGATPLPDKQEGALFFFPRLNDKGEPLLTPASKELTFHSLIRPVVNADSLS
                     QKYRFDVTKMVRGGEVVF"
     gene            1873..2301
                     /gene="ndk"
                     /locus_tag="VGB76_03355"
     CDS             1873..2301
                     /gene="ndk"
                     /locus_tag="VGB76_03355"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY0097971.1"
                     /translation="MSEQIGNLTFGIIKPDAVRNGKTGSIIQRITDSGFKLRGMKLIH
                     LTRKEAEGFYAVHRERPFFGELTEFMSSAPCVVLALEKAGAVKAWRDLMGATDPAKAD
                     EGTLRKEFATSIGENAVHGSDSEENARIEISYFFSQLELI"
     gene            2413..>2632
                     /locus_tag="VGB76_03360"
     CDS             2413..>2632
                     /locus_tag="VGB76_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097972.1"
                     /translation="MSIISDVIENTKAIIKGMSVTFSYIPKEKWTVQYPDEPVTMQPR
                     YRGQHLLHVDELGREKCVACFLCAAACPS"
BASE COUNT          568 a          767 c          817 g          480 t
ORIGIN      
        1 ccccgcgcct tgctatactg ccccttcttt ttacaggcaa gccattacca tatctgcggg
       61 cgtcagttga ttttaccggc tggctaagtc tgtccgggac aactcgacga gtggccgaag
      121 cgtggccgcc tgccgttctt caacaaaatg agggagcgaa aactttgagc gtactggttg
      181 ataaaaacac gcgccttgtc gtgcagggca tcacgggcaa agaaggcagt tttcacacga
      241 agcagatggt ggagtacggc acgaacgtcg tcggcggcgt gacgccgggg aagggcggga
      301 cgacgcacga gggcatcccc gtcttcaaca ccgtcgagga agcggtcaga gaggcgggcg
      361 caaacgtctc gatcatctac gtgccgccgc cgtttgccgc cgacgcgatc atcgaagccg
      421 ccgctgccgg aatgccgctc gtcgtctgca tcaccgaagg catcccggcc attgacatgg
      481 tcggcgtgaa gcgtttcata gaaggcacgg ggacgcggct catcgggccg aattgtcccg
      541 gcatcatctc gcccggcaaa tgcaagatcg gcatcatgcc cggccacatc caccgtgaag
      601 gaaacatcgg cgtcgtctcg cgttcgggga cgttaacgta tgaagcggtc gggcagttga
      661 ccgcgctcgg actcggccag tcaaccgcca tcggcatcgg cggcgacccc atcatcggca
      721 cgacgcacac ggacgcgctc gcgctcttcg aggccgaccc cgaaaccgaa ggcatcgtca
      781 tgatcggcga gatcggcggg acggcggaag aggaagccgc cgcctacgcg aaagcgcacg
      841 tgactaaacc catcgtcgcc ttcatcgccg ggcagaccgc cccgccggga cgccgcatgg
      901 gacacgcggg cgcgatcatc gcgggcggca aaggcactgc cgccgagaag atgtccgcgc
      961 tcgaagccgc gggcatccgc gtcgtgcgct cgcccgcaga catcggcacg gccatgcgcg
     1021 acgcgctcgg cgcgagcgcg tctgcgtaag acgcggcgaa acttctgtca gaattacatc
     1081 ttgcggagtc tctggaaatg aagcgttacg gactgcggct cgtaatatta gttgctctcg
     1141 ccgcgcaggc gtctggcgtg gggatgccgt ttgccggggc gcagcagaat tgggagaaag
     1201 ctcctttcca gaattggggc gcagccgacg tgaaggccgt gctcgaaaag tcgccttgga
     1261 gtcgcgtcat cgagcagaac acggtgattg ctccgtcact tctcggcgtg aacgctccga
     1321 aaatggagga gaagacgatc atcctgttac gctcggctct gcctgtccgt caggctttgc
     1381 tgcgcgagag gcagttggct agcaagtatg agagcatgaa cgcggcggat cgcgcggcct
     1441 tcgacgcgaa gaataaagca ctgttggact gtccggcctg cgccaggtat tacgtgatct
     1501 cgattaagtg caactatgcg tgctacggcg ggagctttgt agaagatcac aagaaatctt
     1561 tctacctgtt gaatgacaag ggcgagcgac gggagttggc gggcgcaacg ccgctcccgg
     1621 acaaacaaga gggcgcgctc ttcttcttcc cgcgcttgaa cgacaagggc gaaccgctgc
     1681 tcacgcccgc gagcaaggaa ttgactttcc actccctgat ccgccccgta gtgaacgcgg
     1741 attcgctaag ccagaaatat agattcgacg tgacgaaaat ggtgcgcggc ggcgaggtgg
     1801 tgttctaaca cgcgcggcgg cgggtgcgcc ctcaattaag ttcgactgaa atcttcttcg
     1861 caagaggtgt caatgtcaga gcaaatcgga aatcttactt tcggaatcat caagcccgat
     1921 gcggtgcgca acgggaagac gggcagcatc atccaacgga tcaccgatag cggtttcaaa
     1981 ctgcgcggga tgaagttgat ccacctgaca cgcaaggagg cggaaggctt ttacgcagtc
     2041 caccgcgagc gtccgttctt cggcgagttg acggagttca tgtcgagcgc gccgtgcgtc
     2101 gtcttagcgt tggagaaagc gggcgcggtc aaagcctggc gcgacctgat gggcgcgacc
     2161 gacccggcca aggccgacga gggcacgctc cgcaaagagt ttgccacgtc catcggcgag
     2221 aacgccgtcc acggctccga ctccgaggag aacgcgcgca tcgagatcag ctacttcttc
     2281 agtcaactgg aattgatcta aagcagaatt caggagtcag aatacagaag tcagaatgta
     2341 gatcatttgc gcccggaacc gcgttgattc cgactcctga attctgactc ctgttttctc
     2401 cgagaggttt acatgtccat catcagcgac gttatcgaaa acacgaaggc gatcatcaag
     2461 gggatgagcg tgaccttctc ttacatcccg aaagagaagt ggacggtgca atatccggat
     2521 gagccggtga cgatgcagcc gcgctatcgc ggccaacacc tgctgcacgt agatgaactc
     2581 gggcgcgaaa agtgcgtcgc ctgtttcctg tgcgcggcgg cctgcccgtc cg
//
LOCUS       DASWNS010000099         4391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_445113, whole genome shotgun sequence.
ACCESSION   DASWNS010000099 DASWNS010000000
VERSION     DASWNS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445113"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VGB76_03365"
     CDS             complement(<1..303)
                     /locus_tag="VGB76_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097973.1"
                     /translation="MTDRREFLKHLIIGGAAAFVAPSLYARDASGSVSNQTLKGGADA
                     WAQVPQILKRIKPPRFAKRDFVITKFGAVADGKSESTEAIRKAIEACSKAGGGRVVV"
     gene            complement(410..1543)
                     /gene="pelA"
                     /locus_tag="VGB76_03370"
     CDS             complement(410..1543)
                     /gene="pelA"
                     /locus_tag="VGB76_03370"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEY0097974.1"
                     /translation="MKKRFTVRAAVLVCCLSAAAFAVPAQKADGAASTQKPTPAAQTI
                     NWRDALKQSAEWYASDEAVRVADNVLLYQHDEGGWSKNIDMARVLTEPERAELAKLKA
                     ETGSNIDNGATYTQLIYLARVYTARKMERHREAFLRGFDYLLKAQYENGGWPQYYPLR
                     KGYYSHVTFNDSAMVNVLRLLRDVARKRSAYRFVDEDRRLRAEKAVAKGIELILKTQV
                     VIGGKRTIWGAQHDEVTLAPAPARKFEPASLSAGESVGVVRFLLGIENPDARIVEAVE
                     SAVAWFKTSGVCGLRWVERRDASKPGGYAREAVADPKAPPIWARFYEIGTNRPVFAGR
                     DGIVRYNVNEIDEERRNGYGWYVEEPAELLEKDYPAWRKKWRP"
     gene            complement(1678..>4391)
                     /locus_tag="VGB76_03375"
     CDS             complement(1678..>4391)
                     /locus_tag="VGB76_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0097975.1"
                     /translation="AYERLKETTAEPQLYTVPTARMRTGDLSEFLSPTASPNGRVLIY
                     NPFSIGATGTANRTPFAGNIIPTALLDPVAVNYLNMYPLPNREGLQNNFFSPTLRFQN
                     YRSWVTRVDHNLNSNHRLFFNYYHSFNPEDRRDWAQTEVNGFPVTRGVEFRTNDGAVL
                     GYTATLSPSMVLDVRGNMTRFVQERRPSGTFDPAELGFTAEALAAMRGYQYLPRFDIR
                     TYDQVRPIRSTLGAERADFNEGFVRPLYVASLQPTLTAIFGNHTARFGYDGRVLRENF
                     SGNGYQGGRFTFDGTFTTITTTTTTNSSNATNHERNRNLYGRDLAAFLLGLPVSGSNS
                     RIENSVNYNVQSIYHGLFVQDDWRVTPKLTLNLGMRYDLELGITERFNRLSRGFDLTS
                     PSPIDAAARAAFTTIYNANQARFAGSGLTPDQFAVRGGFTYANDNDRAAWDADRSNLQ
                     PRIGAAYQLNDKTVLRGGFGIFTSAFRIPSTLSDIANNSLTIQPGFSGFTPFVPSTNS
                     GRTFIASLDNPFPSGLRPSAGTSLGLLTNFAQTLGASDAPILPTERKNAKFARLIIGM
                     QRELPGQFIVEANFVSSWGYDLAVNRNINFVPAGLLGTDPTSAETANTFLTGNDPNNP
                     FRNLLNNNLFLSDASFNTGATVDLLQLVRPYPQFGDIWIQEYNGTNRYTSLQLQGQKR
                     FSRSFSLTTSYTYSRLKEKITYLNASDTELEERFSTDDRPHRFTFAGVYELPFGRGRR
                     FGHDMNRIVNAFIGGWQLNGTYEWQSGEPLLFGDNFYYAGNINALSSRVGQYNEQGRR
                     YGVDIPAFDTAGWVELPSTGLRNVPSTLPKLRNQPFKSVNMSLSKNFNFNERMRLQFR
                     AEALNALNYVYFGGINLADGETNANFGRVNNQRNLPRDIQLGLKFTF"
BASE COUNT          755 a         1257 c         1488 g          891 t
ORIGIN      
        1 cacgaccacg cgcccgccgc cagccttgct gcacgcttca atggctttgc ggatcgcctc
       61 cgtgctctcg ctcttcccgt ctgcgacggc tccgaacttc gtgatcacga aatcgcgttt
      121 cgcaaagcgc ggcggcttga tcctctttag aatctgcggc acctgcgccc acgcgtccgc
      181 cccgcccttc aatgtttgat tcgagacgct cccggaggcg tcgcgtgcgt agagcgaggg
      241 cgcgacgaaa gcagccgctc cgcctatgat caagtgtttg agaaactcgc gtcggtctgt
      301 catatctcac ctatgattga ttcggagact cgcaaagcga cgggcagcac gcgcctcgcg
      361 cgcagcctct ttgcactaca ttagattagg gacactatta gtgacgccat tagggacgcc
      421 acttcttgcg ccacgccggg taatctttct caagcagttc ggcaggctct tcgacgtacc
      481 agccgtagcc gtttcgacgt tcctcgtcaa tctcgttgac gttgtagcgg actatgccgt
      541 cgcgtcccgc gaagacggga cggttggtgc caatctcgta gaagcgcgcc cagatgggag
      601 gagccttcgg gtcggcgacc gcttcgcgcg cgtagccgcc gggcttcgat gcgtcgcgcc
      661 gctcaaccca gcgcaggccg cagactccag acgttttgaa ccacgcgaca gccgactcga
      721 cggcttcgac gatgcgcgcg tcggggtttt cgatccccag caggaagcgt acgacgccga
      781 cgctctcccc tgcgctcaaa gaagcgggtt cgaacttgcg tgcaggggcg ggcgcgagcg
      841 tcacctcgtc gtgctgcgct ccccagatgg tgcgcttgcc gccgatgaca acctgcgtct
      901 tgaggatcaa ctcgatccct tttgccaccg ccttctcggc gcgcagtcgc cggtcttcat
      961 ccacgaagcg ataggccgaa cgcttccgcg ccacgtcgcg cagcagcctc aagacgttga
     1021 ccatcgcgct gtcgttgaag gtgacgtgcg agtagtagcc tttgcggagc gggtagtatt
     1081 gcggccagcc gccgttctcg tattgggctt tgagcaggta atcgaaaccg cgtagaaatg
     1141 cttcccggtg ccgctccatt ttgcgtgcgg tgtagacgcg cgcgaggtag atgagttgtg
     1201 tataggtcgc gccgttgtct atgttcgagc cggtttccgc tttgagcttg gcgagttcgg
     1261 cgcgctccgg ctctgtcagc acgcgcgcca tgtctatgtt cttcgaccag ccgccttcgt
     1321 cgtgctggta gaggagaacg ttgtcggcga cgcgcaccgc ctcgtcgctc gcgtaccact
     1381 ccgcgctttg cttgagggcg tcacgccagt tgatggtttg cgccgccggg gtcggcttct
     1441 gcgtcgaggc agccccgtcg gccttctgcg ccgggacggc gaacgccgct gccgataaac
     1501 aacagacgag aaccgccgcc ctcactgtaa atcgtttctt cattccgcct ctagcgagtt
     1561 gagactgtct gctccgaacg aatcgaagat gcggcgtgag ccgcccgggc tgcttgcacg
     1621 gagcggctca cgtctctgtt agtggccgga aacttaatcc gtccacgtca aggggtttta
     1681 gaaagtgaac ttcaagccga gttggatgtc gcgcggcagg ttacgctggt tgttcacccg
     1741 accgaagttc gcgttggttt cgccgtcggc gaggttgatg ccgccgaagt agacatagtt
     1801 tagagcgttc aacgcctcgg cgcggaactg caagcgcatc ctctcgttga agttgaaatt
     1861 cttggacaac gacatgttga cgctcttgaa cggctggttg cgcagcttgg gcagcgtcga
     1921 gggcacgttg cgcaggccgg tcgagggtag ttcgacccag cccgccgtgt cgaacgcagg
     1981 gatgtctacg ccgtagcggc gaccctgctc gttgtactgg ccgacgcggc tcgacagcgc
     2041 gttgatgttg cccgcgtagt agaagttgtc gccgaacaga agcggctcgc cgctctgcca
     2101 ctcgtaggtg ccgttgagct gccatccgcc gatgaaggcg ttgacgatgc ggttcatgtc
     2161 atgaccgaac ctgcggccac gaccgaaggg cagttcgtag acgcccgcga acgtaaagcg
     2221 gtgcgggcgg tcgtcggtcg agaagcgttc ttccaactcc gtgtcggagg cgttcaggta
     2281 ggtgatcttc tctttcagac gcgagtaggt gtagctcgtc gtgagcgaga agctgcgcga
     2341 gaaacgcttc tggccttgaa gctggagcga ggtgtagcgg ttggtgccgt tgtactcctg
     2401 aatccagatg tcgccgaact gcgggtacgg cctcaccagt tgcagcaggt ctaccgtcgc
     2461 gccggtgttg aaagaggcgt ccgagaggaa gaggttgttg ttgagcaggt tcctgaaagg
     2521 gttgttcggg tcgttgccgg tcaggaaagt gttggccgtc tccgcgcttg tcgggtcggt
     2581 gccgagcagc ccggcgggca cgaagttgat gttgcggttg accgccagat cgtaacccca
     2641 cgaagagacg aagttggcct cgacgatgaa ctggccgggc aactcgcgct gcatgccgat
     2701 gatcaggcgt gcgaacttcg cgttcttgcg ctccgtcggg aggatgggcg catcggacgc
     2761 gccgagcgtc tgcgcaaagt tggtcagcag tccgaggctc gtgcccgccg aggggcggag
     2821 gccgctcgga aacgggttgt cgagcgaggc gatgaaggtg cgaccgctgt tggtcgaggg
     2881 cacgaagggc gtgaagccag agaagcccgg ctgaatggtc agcgagttgt tggcgatgtc
     2941 ggagagcgtg ctcgggatgc ggaaggcgga cgtgaagatg ccgaagccgc cgcgcagcac
     3001 ggtcttgtcg ttgagttggt aagctgcgcc gatgcgcggc tgcaagttcg aacggtcggc
     3061 gtcccacgcc gcgcgatcgt tatcgttggc ataggtgaac ccgccgcgca ctgcgaactg
     3121 gtcgggcgtc agcccggagc cagcgaagcg tgcctgattg gcgttgtaga tggtggtgaa
     3181 agcggcacgc gcggctgcgt cgataggact cggagaggtc aggtcgaagc cgcgtgagag
     3241 acggttgaag cgttccgtga tgccgagttc cagatcgtaa cgcatgccga ggttcaatgt
     3301 gagcttcggc gtcacgcgcc agtcgtcctg cacgaataag ccgtgataga tggactgcac
     3361 gttgtagttg acggagttct cgatgcggct gttgctgccg gagacgggca ggccgaggag
     3421 gaacgcggcc aggtcgcgcc cgtaaaggtt gcggttccgt tcgtggttgg tcgcgttgct
     3481 gctgttggtc gtggtcgtgg tcgtgatggt cgtgaacgtg ccgtcgaagg tgaagcggcc
     3541 accctgataa ccgttgccgc tgaagttctc gcgcagcacg cggccatcgt aaccgaacct
     3601 cgcggtgtgg ttgccgaaaa tcgcggtcag cgtgggctgg agcgacgcca cgtagagcgg
     3661 gcggacgaag ccctcgttga agtcggcacg ctccgcgccg agtgtggagc ggatcggcct
     3721 cacctgatcg taagtgcgga tgtcgaaacg cgggagatac tggtagccgc gcatggcggc
     3781 cagagcctcg gcggtgaagc cgagttcagc cgggtcgaac gtcccggaag gccgacgctc
     3841 ctgcacgaag cgcgtcatgt tgccgcgcac gtcgagcacc atcgaaggcg agagcgtggc
     3901 ggtatagccg aggacggctc cgtcgttggt gcgaaactcc acgcctctcg tgacggggaa
     3961 gccgttcacc tcggtctgcg cccaatcgcg acgatcttcc gggttgaagc tgtgatagta
     4021 gttgaagaag agcctgtggt tcgagttgag attatggtca acgcgcgtta cccacgagcg
     4081 atagttctgg aagcgcagcg tcggcgagaa gaagttgttc tggagtcctt cacggttcgg
     4141 cagcgggtac atgttcaggt agttgacggc gaccggatcg agcagtgcgg tcgggatgat
     4201 gtttccggcg aagggcgtgc ggttggccgt gcccgtcgcg ccgatgctga acgggttgta
     4261 gatcagcacg cgtccgttcg gcgaagccgt cgggctgagg aactccgaga ggtcgccggt
     4321 gcgcatgcgc gcggtcggca ccgtgtagag ttgcggctcg gcggtagtct ccttcaaacg
     4381 ctcgtaggcg a
//
LOCUS       DASWNS010000100         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_108171, whole genome shotgun sequence.
ACCESSION   DASWNS010000100 DASWNS010000000
VERSION     DASWNS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108171"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..315
                     /locus_tag="VGB76_03380"
     CDS             73..315
                     /locus_tag="VGB76_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY0097976.1"
                     /translation="MAHIKMYTTTWCSDCRHAKRFLNESGIAYEEINIEEHDGAAELV
                     MRLNEGKRKVPTFEVDGRAFNLSPYDERKLRAELGL"
     gene            353..637
                     /locus_tag="VGB76_03385"
     CDS             353..637
                     /locus_tag="VGB76_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097977.1"
                     /translation="MDETNTKRNGTGRQLTRKVARAGGLRLVRRLIKPIPIIGTAVAL
                     GFAGHEIRKKGWRNGLIHVGLDITPVVGTVKDVIEIFTGDLIPDKENVKR"
     gene            complement(846..>1584)
                     /locus_tag="VGB76_03390"
     CDS             complement(846..>1584)
                     /locus_tag="VGB76_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0097978.1"
                     /translation="PDYVSELTGDVRGARIGVPRELFGEGLNPDVRASVEAAIETYRT
                     LGAEVVDIELPYAKYAIAVYYIIATAEASSNLARYDGVRYGFRAEETPTLREMYRRTR
                     EEGFGAEVKRRIMLGTYVLSSGYYDAYYLKAQKVRTLLKQDFARAFERCDAIITPTSP
                     TTAFLLGEKTDDPLAMYLNDIYTVTANLAGIPGLSIPCGLSSSGLPIGMQLLGAHWSE
                     PTLFRLAEAYERAQPFKARPPVMAKEQ"
BASE COUNT          331 a          463 c          496 g          294 t
ORIGIN      
        1 tgtgttttgt ctcgccgcgc ccgtccgcta caataacgac atcatttttc attccaggag
       61 agaactctcg cgatggcaca catcaagatg tatacgacta catggtgctc cgattgccgc
      121 catgccaaac gtttcctgaa cgagagcggc atcgcttacg aggagatcaa catcgaagag
      181 catgacgggg cggcggaact cgtgatgcgg cttaacgaag gcaagcgcaa agttccgact
      241 tttgaagttg atgggcgcgc gtttaacctc tcgccctacg acgaacgaaa actacgcgcg
      301 gaactcggcc tctagccgaa acggcgggaa ccgcaaggga tgggcgacag cgatggatga
      361 aacgaacacg aagcgaaacg gaaccgggcg acaactgacg cgcaaggtgg cgcgcgccgg
      421 gggcttgcgc ctggtgcgtc ggctcatcaa gccgatcccc atcatcggca ctgccgtcgc
      481 gctcggcttc gccggacacg agatcagaaa gaagggctgg cgcaacggcc tcatccacgt
      541 cggcctcgat atcacacccg tcgtcggcac agttaaagac gtgatcgaaa ttttcacggg
      601 cgatctgatt cccgacaagg aaaacgtcaa acgctagctc ttgaggcgag gttaatcagg
      661 taagttcttg aagccgcgac gcgccgcgag atgcgcgatg atcccgccga cgaacacact
      721 cgcttctcaa actatcagtc agttgaacgc gccacctgcg acgagaagaa tagttcgctg
      781 catgtcgatc tctccctgtg ccgcacgcgc gctgtcagac caaactcatc tcagttaaag
      841 agagttcatt gttccttcgc catgacgggc gggcgcgcct tgaagggctg cgctcgctcg
      901 taggcttcgg caaggcggaa gagcgtcggt tcgctccagt gcgccccgag cagttgcatg
      961 ccgatgggca gacctgagct tgagaggccg caggggatgg agaggccggg gatgcccgcg
     1021 aggttggccg tcacggtgta gatgtcgttc aagtacatcg cgagcgggtc gtcggtcttt
     1081 tcgccgagca ggaaggctgt ggtcggcgaa gtcggcgtga tgatggcgtc gcaacgctcg
     1141 aaggcgcgtg cgaagtcttg tttgaggagc gtgcgtacct tttgcgcctt gagatagtag
     1201 gcatcgtaat agccggacga gagcacatac gtgccgagca tgatgcggcg tttgacctcc
     1261 gcgccgaaac cttcctcgcg cgtgcgtcga tacatctcgc ggagcgtggg cgtctcttcg
     1321 gcgcgaaagc cgtagcgcac gccgtcgtag cgcgcgaggt tggaagaggc ttcggcggtg
     1381 gcgatgatgt agtagaccgc gatggcgtat ttcgcgtagg gcagttcgat gtcaaccacc
     1441 tccgcgccga gcgtgcgata agtctcaatc gccgcctcca cgctcgcgcg cacgtccggg
     1501 tttaagccct cgccgaacaa ctcgcgcggc acgccgatcc gcgcgccgcg cacgtcgccc
     1561 gtcaactccg agacgtaatc gggc
//
LOCUS       DASWNS010000101        10144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_341441, whole genome shotgun sequence.
ACCESSION   DASWNS010000101 DASWNS010000000
VERSION     DASWNS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10144
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341441"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VGB76_03395"
     CDS             <1..562
                     /locus_tag="VGB76_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY0097979.1"
                     /translation="AESYTQGDARRRAELLAWADEVARRPPTGTINCLALDAASDISG
                     VTTTSGLAWKIPGRVGDSPLIGCGLYVDNDTGAAGSTGRGEEVIYINGARSVVEFMRR
                     GASPEQACLDALKLIAARYGNDLEKLKDIDVNFYALNKRGEFAGAAIWNGTRNSRGEL
                     RRRQFAAHDGREGRLFESAYLFERRA"
     gene            867..1586
                     /locus_tag="VGB76_03400"
     CDS             867..1586
                     /locus_tag="VGB76_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097980.1"
                     /translation="MISRNWSRMAISLCLTVAILSTFSMVALAAQAQSASGELSVVGE
                     VSVNGTNAISGATVFSDSTVTTAKGSSAVVSLGKLGRVEVLPESSIKLSFTDNSVTGT
                     LEAGRVRYSTSAGTAASVITRDGSAVADNSQPNVFTVNVECGNTEVATQSGRVELRAG
                     NSVKQVAAGNQDTAGTAQPGSRCTRLTRSTDFGALSGGALAALLLAAGGAVAAAIIAA
                     TDDSNDLGVGGTAPVFSPSRQ"
     gene            1745..3091
                     /locus_tag="VGB76_03405"
     CDS             1745..3091
                     /locus_tag="VGB76_03405"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY0097981.1"
                     /translation="MQIAVIGTGYVGLVTGACFAEFGVDVVCFDIDTRKIDELNQGKI
                     PIYEPGLEQLVEKNAQAGRLKFTTDLKSAVEPSLVILLAVGTPQGTDGSADLSYVEAA
                     ARAVAEHITDYKVIVTKSTVPVGTGEHLRSIIREQNKQAKFGMVSNPEFLREGAAIGD
                     FMRPDRVVIGSRDAEAIAIVKDLYRPLYLIETPFVITSMEGAELTKYAANAFLATKIS
                     FINEIANLCDRIGCDVHDVARALGMDNRIGKKFLHPGPGFGGSCFPKDTNAFLSIAGK
                     YDYDALIVDAVVRVNERQRGLMVPKIERLVGGDLRSKTVAVLGLAFKPGTDDMREAPS
                     VDIIRLLLERGAAVRAYDPIAMNAAREFLPGITYVEDEYEAVEGADVLVFMTEWNQFR
                     ALNMERVRELMRAPRIADLRNIYEPEAMCELGFEYVGVGRQPRAATANDKGQSETA"
     gene            3152..4108
                     /locus_tag="VGB76_03410"
     CDS             3152..4108
                     /locus_tag="VGB76_03410"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0097982.1"
                     /translation="MSLSGIVLVTGGAGFVGSHIAATFAGRGARVRVIDDLSTGHLEN
                     LEEIGGDIDFVRGSLNDEATLRRALEGVELVFHEAAIPSVPRSVENPRETHRACAEAT
                     FSLLLAARESGVRRVVYAASSSAYGDQPTLPKVETMPPDPLSPYAAAKLIGEYYCQVF
                     TRTYKLETVALRYFNVFGPRQDPSSTYSGVISRFIAALSTGQTPVIYGDGEQSRDFTY
                     VANVVDANLRAAETTRGVGQIINVANGQRVTLNELLEQLKQLTGHADVQAEYRDARVG
                     DVKHSLADITRAREFLGYEPQVGLEEGLRHTIDWWKQSRFAR"
     gene            complement(4244..5296)
                     /gene="mtnA"
                     /locus_tag="VGB76_03415"
     CDS             complement(4244..5296)
                     /gene="mtnA"
                     /locus_tag="VGB76_03415"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEY0097983.1"
                     /translation="MIKTVEWTAEGVRMIDQRLLPTEEKYLILRSCEEVADSIRDMAV
                     RGAPAIGVAAAMGIAVGLKQAVATAVADLQYDFDHMCNMMSQTRPTAVNLFWAIERMR
                     EAFRRAAAATSDVEEIKRRMVDEAKAVYAEDIASNRALGRFGGELLPDGANVLTHCNA
                     GALATAGDYGTALGVIRGARDAGKRIAVIADETRPFLQGSRLTAWELDQDDIPVTLIT
                     DNMAGHVMKQGKVDAVVVGADRIAANGDTANKIGTYMVAVLARQHNIPFYVAAPISTV
                     DLNIPKGEEIPIEERNTREVTHIHEHRLAPEGINVQNFAFDVTPNEFIAAIITDKGIA
                     RAPYTESLRKLFGEQN"
     gene            complement(5553..5975)
                     /locus_tag="VGB76_03420"
     CDS             complement(5553..5975)
                     /locus_tag="VGB76_03420"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEY0097984.1"
                     /translation="MAKTVATIMGVVFLLVGIMGFVAPGVGGFHLSTAHNLVHIISGA
                     LALYFGLAGTLSGARLFCIIFGVVYGLLGVVGFLLGAPGTPSMPGMAADSRLFKVLPG
                     TLELGTSDHVFHILLGLIFLIAGFLTKAAYDARTRDDH"
     gene            6323..6544
                     /locus_tag="VGB76_03425"
     CDS             6323..6544
                     /locus_tag="VGB76_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0097985.1"
                     /translation="MGDVREPPVRVSPEEAWRLVAEGRAPVFLDTRNARHYAQSDVQI
                     PGSLYIWRGELEARIGEVPRGRPIVTYCA"
     gene            6773..8251
                     /gene="gatB"
                     /locus_tag="VGB76_03430"
     CDS             6773..8251
                     /gene="gatB"
                     /locus_tag="VGB76_03430"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY0097986.1"
                     /translation="MKHGWEAVIGLEIHAQLNTESKIFCGCSTRFGDEPNANTCPVCL
                     GLPGALPVLNRRVVELGARAALALGLRINEQSIFARKNYFYPDLPKGYQISQFDRPFS
                     EHGELEIMTATRDEAGHARDWRPMLIRITRMHLEEDAGKNVHEGLPETERYSYVDLNR
                     AGTPLAEIVTEPDFRTSWEAYDYVNHVRRALQWVGASEADMEKGNLRCEANVSVRRVG
                     EEKFGTKVELKNLNSVRFMQKAIEFEIARHIELIEGGGRVTQETRLWDDRVGETRVMR
                     SKEEAHDYRYFPEPDLPPLVVTSAFIEEVTAQMPELPEARRRRFMEEYALSFEDASQF
                     TSERGLADYYERAARAANNPRGAANWIRSELLRELEATGRTAEASPVAAEELGALVRL
                     IDEGKISGKQGKEVLVEMFASGKSASVIIEEQGLAQVSDAGEIATLVDEVIAASPKQL
                     EQYRAGKETLFGFFVGQVMKASKGKANPKLVNEVLQEKLREQ"
     gene            8334..9056
                     /locus_tag="VGB76_03435"
     CDS             8334..9056
                     /locus_tag="VGB76_03435"
                     /inference="COORDINATES: protein motif:HMM:NF005594.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0097987.1"
                     /translation="MNLDGKVAIVTGSTKGIGRAIAEALVREGLNVCISARSEDEVER
                     AVGELGDAGGGRVTGAVCDVRSYEEVRALFEHTVAEFGGVDVLVNNAGIGLFQSVEEM
                     SPEDFRAVLETNLNSVFYACHEAIPRMRERGGGYIINISSLAGANAHPRMAAYNASKF
                     GLNGFSEALMQEVRHDRIKVSYIMPGSVNTYFGGDVPSAEQSWQLQPADVARVVLDLL
                     KHDARSLPSRVEIRPSMPPKAK"
     gene            9069..9902
                     /locus_tag="VGB76_03440"
     CDS             9069..9902
                     /locus_tag="VGB76_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0097988.1"
                     /translation="MRRLILALLVCLIPPALHAQSASPTPAVPAQAKATSPVQSAEFA
                     EAERLNASVLQLYAAGKYKEALPLAERVLALREKASGDEDAFVGSALNNLATLYLTLK
                     HSDKALPILERILARQEKLKTASSRMTMTLIMSYICLTSAKGTSLRVRGVKPSERRVT
                     LTDRINAILLQDAILAAGLAVPENLSELSADIITRKPQPRYSAEAKSARLQGSVVVLA
                     EADETGKVLSAEPVECWIGQKPLADAAAEALREARFKQVSINGKPIKLKAIAMYNFVL
                     R"
     gene            9943..>10144
                     /locus_tag="VGB76_03445"
     CDS             9943..>10144
                     /locus_tag="VGB76_03445"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HEY0097989.1"
                     /translation="MEAGDVAEQLIELKEGEGDREENDQFRSRVALTISFMAMLLAIT
                     SLWGGNAAEDIMNHNIHASDTWA"
BASE COUNT         2119 a         2846 c         3210 g         1969 t
ORIGIN      
        1 cgctgagtcg tatacacagg gcgacgcgcg gcgacgcgcg gagttgctgg cgtgggcgga
       61 tgaagtcgcg cgccgtcccc cgaccggcac gatcaattgt ctcgcgctcg acgccgcaag
      121 cgacatcagc ggcgtgacga cgacgagcgg gctggcctgg aaaattccgg ggcgcgtcgg
      181 cgattcgccg ctcatcggct gcggcctcta cgtggacaac gacacgggcg cggcgggttc
      241 gaccgggcgc ggcgaagaag tgatctatat taacggcgca cgcagcgtcg tcgaattcat
      301 gcggcgcggc gcgtcgcccg aacaagcctg cctcgacgcg ctgaaattga tcgccgcgcg
      361 ttacggcaac gacctcgaaa aattgaaaga tatagacgtg aacttttacg ccctgaacaa
      421 gcggggcgag tttgcgggcg cggccatctg gaacggcacg cgaaatagtc gcggggaact
      481 gcggcggcgg caattcgccg cgcacgacgg gcgggaaggg cgtctcttcg agagcgcgta
      541 tctgtttgag cggcgggctt gaagtcggct atacacgtcc gcgtgtctgc ccggagcgtg
      601 tgcgagcgga caaaaaagcc aagaaaaagc tgagaattta gctaaatgtt tattgacagc
      661 aaacggggat tgatgtaaaa aggctaggtc aaagcagccc acagaggctc gctgacctgc
      721 tttcttaacg aatgctacag ggacgcaatt gaatttcagg cgggtttagc gaggaaatat
      781 cagaatccgt cttctgcgaa gttctctcta aggactcgcg gcgcgctctc tacaaacact
      841 ggttcagtct acttgggagg agaataatga ttagcagaaa ttggagccgt atggctattt
      901 cgctctgcct gacagttgcc attttgagca cgttctcaat ggttgctctg gcggcgcaag
      961 cacagtcggc ctcgggcgaa ctgtcagtcg tcggcgaagt ttccgtaaac ggaacgaacg
     1021 cgatctccgg tgcgacagtt ttttcggaca gcaccgtcac caccgccaaa ggttcgagcg
     1081 cagtcgtgag tttaggtaaa ctcggacgcg tcgaagtctt gccggaatcc agcattaaac
     1141 tcagctttac agacaacagc gtcacgggca cgcttgaggc cgggcgcgtg cgctattcga
     1201 cctctgcggg cacggcggcc agcgtcatca ccagagacgg ctcagccgtg gccgacaaca
     1261 gccagccgaa cgtcttcacc gtgaacgtcg aatgcggcaa cacggaagtc gccacccaat
     1321 cgggtcgcgt cgaactccgc gccggcaaca gcgtcaaaca ggttgctgcc ggtaatcagg
     1381 acaccgccgg aacggctcaa ccgggttcgc gctgcacgcg cctgacccgt tcgaccgact
     1441 tcggtgctct cagcggcggc gcgctcgcgg cgcttctgtt ggcggctggc ggcgcggttg
     1501 cggcggccat catcgcagcg accgacgata gcaacgacct tggcgtcggc ggcaccgctc
     1561 cggttttcag cccgagcagg cagtaagttt cgccggctgt tgagttgtta ggctttgaca
     1621 gctgtgcgat cttctcgcgc acggtagacg agaacattca cttgcgggtc acgcgcgggt
     1681 ttaccccgcg tgacccgcaa acttttgtct gaatgtatca atccttgaaa ggtctgtttt
     1741 aaagatgcag attgcagtga tcgggacggg ctacgtcggc cttgtgaccg gagcctgttt
     1801 cgccgagttt ggcgttgacg tggtgtgttt cgatatcgac acgcggaaga tcgacgaact
     1861 caatcaaggt aagattccga tttacgaacc cggccttgag caactcgtgg agaaaaacgc
     1921 gcaggccggg cgtttgaaat tcacgaccga cttgaagagt gcggtcgaac cttcgctggt
     1981 gatcctgctg gccgtcggga cgccgcaggg aacggacggc tctgccgact tgagctacgt
     2041 ggaagcggca gcccgcgcgg ttgccgaaca tatcaccgat tacaaagtga tcgtcacgaa
     2101 atccaccgtc cctgtcggta cgggcgagca tctgcgcagc atcatcaggg agcagaataa
     2161 gcaggccaaa ttcggcatgg tttcgaaccc ggagttttta cgcgaggggg cggccatcgg
     2221 tgatttcatg cgtcccgacc gcgtcgtgat cgggagtcgc gatgcggagg ccatcgccat
     2281 cgtcaaagac ctctatcgtc cgctctactt gatcgagacg cctttcgtca tcacctcgat
     2341 ggaaggcgcg gagttgacga agtacgccgc caacgctttt ctcgccacga agatttcctt
     2401 catcaacgaa atcgccaacc tctgtgatcg catcggctgc gatgtgcacg atgtcgcgcg
     2461 cgccctcggc atggacaacc gcatcggcaa gaaattcctc catccgggac ccggcttcgg
     2521 cggttcctgt tttcccaaag acaccaacgc gtttctctcc atcgccggca aatatgatta
     2581 cgacgcgctc atcgtggatg cggtcgtgcg tgtcaacgag agacagcgag ggttgatggt
     2641 gccgaagatc gagcgtctgg tgggcggcga tttacgcagc aagaccgtcg ccgtgctcgg
     2701 cctggcgttc aaaccgggaa cggacgacat gcgcgaagcg ccctccgtag acatcattcg
     2761 tctcctgctc gaacgtggcg cggcagtgcg cgcttacgat ccgattgcga tgaatgccgc
     2821 gcgtgaattt ttgccgggca tcacctatgt cgaagatgaa tatgaagcgg ttgaaggcgc
     2881 agacgtgctc gtcttcatga ccgagtggaa tcagtttcgc gcgctcaaca tggaacgcgt
     2941 gcgcgaactc atgcgcgctc cgcgcatcgc cgatttgcgc aacatctacg aacctgaagc
     3001 catgtgcgaa ctcggcttcg aatacgtcgg cgtcggcaga cagccccgcg ccgccactgc
     3061 gaatgacaag ggacaaagcg aaacggcctg atctccgcgt ccgtcatccg aaacagactg
     3121 atctcctgac tcgccgcccg acactaaact tatgagtctt tctggaatcg tactcgtgac
     3181 cggcggagcc ggtttcgtcg gctcgcacat cgccgcgact ttcgcggggc gcggcgcacg
     3241 cgtgcgcgtc atagacgatc tctcgaccgg ccatctcgaa aatctggaag agatcggcgg
     3301 agacattgat ttcgtgcgcg gcagtttgaa cgacgaagcg acgctcaggc gcgcgctcga
     3361 aggcgtcgaa ctcgtttttc acgaagcggc tattccctcc gtgccgcgtt cggtggagaa
     3421 cccgcgcgag acgcatcgcg cctgtgccga ggcgacgttc tctctgctcc tcgcggcgcg
     3481 tgagagcggc gtgcgccgcg tcgtctatgc cgcttcgagt tcggcctacg gcgaccagcc
     3541 gacgctgccc aaagtcgaga cgatgccgcc cgatccgctc tcgccttacg ccgccgcgaa
     3601 actcataggc gagtattact gtcaggtctt cacgcgcacg tacaaactcg aaaccgtagc
     3661 cctgcgctac ttcaacgtct tcggaccacg ccaagacccg agttcgactt actccggcgt
     3721 catctcacgt ttcatcgccg cactctcgac cggacaaacg cccgtcattt acggcgacgg
     3781 cgagcagtcg cgcgatttca cttatgtcgc caacgtcgtc gatgcaaacc tgcgcgccgc
     3841 cgaaaccacg cgcggcgtcg gccagatcat caacgtcgcc aacggccagc gggtgacgct
     3901 caacgaattg cttgagcagt tgaagcaact cacagggcac gcggacgtgc aggcggagta
     3961 tcgcgatgcg cgcgtcggcg acgtgaagca cagcctcgcg gatatcacac gcgcacgcga
     4021 gtttctcggc tacgaaccgc aggtcggtct ggaagaggga ttgcgccaca cgattgactg
     4081 gtggaagcag agccgcttcg cgcgttagtc acgagataaa gaaaagcgca cgacgcggag
     4141 acgctgagtt aacgcagcga ggggacgcag aaacagttga tcattcaact gtttctgcgt
     4201 cccctcgctg cgtctctctc tttgcgtctc tctctgttga agcttaattc tgttcgccga
     4261 acagctttcg caaactctcc gtgtaaggtg cgcgtgcgat gcctttgtcg gtgatgatgg
     4321 cggcgatgaa ttcgttgggc gtgacatcga aggcgaagtt ctgcacgttg atgccttcgg
     4381 gggcgagacg gtgttcgtgt atatgcgtca cttcgcgggt gttgcgttct tcgatgggaa
     4441 tctcctcgcc tttggggatg ttcaaatcga cggtcgagat gggcgcggcc acgtagaaag
     4501 ggatgttgtg ctgacgtgcg aggacggcga ccatgtaggt gccgatcttg ttggccgtgt
     4561 cgccgtttgc ggcgatgcgg tctgcgccga cgacgacggc gtcaaccttg ccctgcttca
     4621 tcacgtgccc ggccatgttg tctgtgatga gcgtgacggg aatgtcgtct tgatcgagtt
     4681 cccatgccgt gaggcgtgag ccttgcagga aggggcgcgt ttcgtctgcg atgacggcga
     4741 tgcgtttgcc cgcgtcgcgc gcgccgcgta tcacgccgag cgcggtgccg tagtctccgg
     4801 cggtggcgag cgcgcccgcg ttgcagtgcg tcaggacgtt tgcgccgtcg ggcaacaact
     4861 cgccgccgaa gcgtccgagc gcgcggttcg aagcgatgtc ttccgcgtag acggctttag
     4921 cttcgtcaac catgcgccgc ttgatctctt ccacgtcgga ggtggcggcg gcggcgcggc
     4981 gaaaagcctc gcgcatgcgt tcgatagccc agaagagatt gacggcggtg gggcgcgtct
     5041 ggctcatcat gttgcacatg tggtcgaagt cgtactggag atcggcgacg gcggtggcga
     5101 ccgcttgttt gaggccgacg gcgatgccca tcgcggcggc gacgccgatg gccggagcgc
     5161 cgcgcacagc catgtctctg atactgtcgg ccacctcttc acatgaacgc aggattaaat
     5221 atttttcttc agtcggcagc aggcgttgat cgatcatgcg cacgccttcg gcagtccatt
     5281 cgaccgtttt gatcatttaa gaaacactct cctgatgatg tttgaaagat gaacgatgcg
     5341 tgttcagacc gtgaaactgc tgctttcatc tttgaatttt gatgtggagc atcttaaccg
     5401 acgtgagttg aggcggcaaa cgtgtggctt ggctcaaaga cgaagaggcc gcaggaggga
     5461 agtccggcaa actgtcgggc gtttgccgtc gcacggccag cgtgctgtat ttgctcctgc
     5521 ggcctctcca cggttcaaac cggcgcgaaa gattagtggt cgtcgcgcgt cctcgcgtcg
     5581 taggctgctt tcgtcaggaa gcccgcgatc agaaagataa gcccgagcag gatgtggaac
     5641 acgtggtcgc ttgtgccgag ttcgagcgtg ccgggaagaa ctttgaacag gcgactatca
     5701 gcggccatgc ccggcatcga aggcgtgccg ggtgcgccga gcaaaaatcc cacgacgccg
     5761 agcagtccgt agacgacgcc gaaaattatg cagaagaggc gtgcgccgga gagcgtcccg
     5821 gccagcccga agtagagtgc cagcgcgcct gaaatgatat ggacgaggtt gtgcgccgtg
     5881 ctcaggtgga aaccgccgac gccgggcgcg acgaaaccca taatccccac gagtaaaaac
     5941 acgacgccca taatggtcgc aacagttttt gccataattt tcatcccctc cttcgggaga
     6001 aaaggtggac accttgtaaa cagcgatcgc cacagcactt cttaccgggc gtcgccgcag
     6061 ttcaaaaatc gaaactgagt ctggatgagc ggattgtata ctaaattcaa cgcgggtgag
     6121 ctagtttttc tttcccacga accggaatcc gagacggttt cgccgcgcct tcggctgaat
     6181 gttatactca atttttcaca caagcaggcc acccgccgcg tataaaggca gccagtagag
     6241 gagtgcgagt tcgatatttt gccccgctgc gtgcgcgacg aagaagaaga gcgcgcttga
     6301 gttcgagcga aagtggagag agatgggaga cgttcgagag ccgcccgtac gtgtcagtcc
     6361 cgaagaagcg tggaggttgg tcgcagaggg acgcgcaccc gtgttcctcg acacgcgcaa
     6421 cgcgcgccac tacgcgcaga gcgacgtgca gattccgggg tcgctctaca tctggcgcgg
     6481 agagttggaa gcgcgcatcg gagaagtgcc gcgcggtcgt cccatcgtca cgtattgcgc
     6541 ctgacactac gaaaattcca gcacccgtgc ggcgctgata cttgaagaat acgatttcac
     6601 ggaactctac attctcgtcg gcggcatcga cgcgtggcgc gaccgtgggc ttccgctcga
     6661 accgaaaggg gctgatagcc cttagttgtc agccggacgt tcggaatacc tgtgcaggac
     6721 agaatcttcc gctaacagct aggagttaag agctaacagc taagagctaa ttatgaaaca
     6781 tggctgggag gccgtcatcg gccttgaaat tcacgcgcaa ctgaatacgg agtcgaagat
     6841 tttttgcggc tgctccacgc ggttcggcga tgagccgaac gcaaacacat gtcccgtgtg
     6901 tctgggattg ccgggcgcgt tgccggtctt gaatcgccgc gtcgtcgaac tcggcgcacg
     6961 cgccgcgctc gccctcggcc tgcgcatcaa cgagcaatcg atcttcgcgc gcaaaaacta
     7021 tttctatccc gacctgccaa agggttatca aatttcgcag ttcgaccgcc cgttctccga
     7081 gcacggcgaa ctggagatca tgaccgccac gcgcgacgag gcggggcacg cgcgcgactg
     7141 gcgacccatg ttgattcgca tcacgcgcat gcaccttgaa gaagatgctg gcaagaacgt
     7201 ccacgaaggt ctgccggaga cggaacgcta ttcctacgtc gatctgaatc gtgcgggcac
     7261 gccgctcgcg gagatcgtga ccgagccgga ttttcgcacc tcttgggaag cctacgatta
     7321 cgtcaatcac gtgcggcgcg cgctgcaatg ggtgggggcg tccgaggctg acatggagaa
     7381 gggcaacctg cgctgcgagg cgaacgtctc tgtgcggcgt gtgggcgaag agaaattcgg
     7441 cacaaaggtt gagttgaaga atctgaactc ggtgcgcttc atgcagaagg ccatcgagtt
     7501 tgagatcgcg cgacacatcg agttgatcga aggcggcggg cgcgtcacgc aggagacgcg
     7561 cctgtgggac gaccgcgtgg gcgagacgcg cgtgatgcgc tcgaaagagg aggcgcacga
     7621 ttatcgttat ttccccgaac cggacttgcc gccgctcgtc gtcacgtcgg cctttatcga
     7681 agaggtgacg gcgcagatgc ctgagttgcc ggaggcgcgc cgccgacgct tcatggagga
     7741 gtacgcgttg tcgtttgaag atgcttcgca gttcacttcc gaacgcggcc tcgcggatta
     7801 ttacgagcgc gcggcgcgtg cggcgaacaa cccgcgcggc gcggccaact ggatcaggtc
     7861 ggaactgttg cgcgaactcg aagcgacggg gcggacggcg gaagcgtctc ccgtcgcggc
     7921 agaggagttg ggcgcgctcg tgcgcctgat tgacgaaggc aagatcagcg gcaagcaggg
     7981 caaagaagtg ctcgtcgaga tgtttgcgtc gggcaagtcg gcctccgtca tcatcgagga
     8041 gcagggactc gcgcaggtga gcgacgcggg cgaaatcgcg acgcttgttg acgaagtgat
     8101 cgcggccagc cccaaacaac tcgaacaata tcgcgcgggc aaagagacgc tcttcggctt
     8161 cttcgtcggg caggtgatga aagcctcgaa gggcaaagca aatccgaaac tcgtcaacga
     8221 agtgctgcaa gaaaaattga gagaacagtg acaggtgaca gataacgagt gacgagttga
     8281 aaactaaaaa cttgtcttta actcgtcact cgtcactgct tttgaggtgt tgaatgaatc
     8341 tggacgggaa agttgcgatt gtgacgggga gcacgaaagg gatcgggcgg gcaattgccg
     8401 aggctctcgt gcgggagggc ttgaacgtct gcatcagcgc gcggagcgag gatgaggtcg
     8461 agcgtgcggt cggcgagttg ggcgatgcgg gcgggggacg tgtgacgggc gcggtgtgcg
     8521 acgtgcgtag ctacgaagag gtgcgcgcac tgttcgagca cacggtcgcg gagtttggcg
     8581 gcgttgacgt gctcgtcaat aacgccggta ttggcctctt tcagagcgtc gaagagatgt
     8641 cgccggaaga ttttcgcgcc gtgttggaga cgaatctcaa cagcgtcttc tacgcctgcc
     8701 acgaagcgat cccacggatg cgcgagcgcg gcggcggcta catcatcaac atctcttcgc
     8761 tcgcgggcgc aaacgctcac ccgcgcatgg ccgcttacaa cgcgtcgaag tttggcttga
     8821 acgggttcag cgaagcgttg atgcaggagg tgaggcacga ccgcatcaag gtcagctaca
     8881 tcatgcccgg ttcggtgaac acgtatttcg gcggcgacgt gccgagcgcg gaacagagtt
     8941 ggcaactgca accggcggac gtggcgcgcg tcgtgctcga tctcttaaag cacgacgcgc
     9001 gaagtttgcc gagtcgcgtg gagatcaggc cgagcatgcc gccgaaagcc aaatgagggg
     9061 ttgtgataat gagaagactg atcctcgcac tgctcgtctg tctgattccc cccgcgctcc
     9121 acgcgcagag cgcgagtccg actcctgccg tcccggctca ggccaaagcc acgtcgccgg
     9181 tgcagtcggc ggagtttgcg gaagcggaga gactcaatgc ctccgtgctc caactttacg
     9241 cggcgggcaa atataaagaa gcgttgccgc tggcagagcg tgtgctggct ctgcgtgaaa
     9301 aagcctcagg ggatgaagac gcgttcgtcg ggagcgcgct caataatttg gcgacgctct
     9361 acctcacgct taaacattcg gataaagccc tgccgattct cgaacgcatt ctcgcgcggc
     9421 aagagaagtt gaaaaccgcc agctctcgga tgacgatgac cttgatcatg agctacatct
     9481 gcctgacaag cgcgaagggc acgagcctgc gcgtgcgagg ggtaaaaccg agcgagcgga
     9541 gggtaacgct gaccgaccgc atcaacgcga ttctcttaca ggatgccata ctcgccgccg
     9601 gactcgccgt tccggagaat ctatcggagt tgagcgcgga catcattacg cgcaaaccgc
     9661 agccgcgcta ttcggcggag gcgaagagtg cgcgcttgca gggaagcgtg gtcgtgttgg
     9721 ccgaagctga tgaaacggga aaggtgttga gcgcggagcc tgtggagtgt tggatcggcc
     9781 aaaaaccatt ggccgacgct gccgccgaag cgttgcgtga ggcgcggttc aaacaggtgt
     9841 cgattaacgg gaaacccatt aagttgaaag ctatcgctat gtataatttt gtgcttcgat
     9901 aaagcgcgaa gcgccgggta atttcagggg aagggatcag acatggaagc gggagacgtg
     9961 gcagagcagc tcattgaatt gaaggaaggc gaaggagaca gggaagagaa cgatcagttt
    10021 cgttcgcggg tggcgttgac gatctcgttt atggcgatgc tgctggcgat cacgagtctg
    10081 tggggcggca atgctgccga agacatcatg aaccataaca tccacgcgag cgacacgtgg
    10141 gcgt
//
LOCUS       DASWNS010000102         5269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_366917, whole genome shotgun sequence.
ACCESSION   DASWNS010000102 DASWNS010000000
VERSION     DASWNS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366917"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..2059)
                     /gene="ftsH"
                     /locus_tag="VGB76_03450"
     CDS             complement(263..2059)
                     /gene="ftsH"
                     /locus_tag="VGB76_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY0097990.1"
                     /translation="MPFSRLLPRTQKTSLLVAIGAVTTLLAVIAFQTFYFSSRPVVQT
                     VNYTELHALTTNDAAVRSLLVEGELLVVEKTDGTRSQAVVTNAVAQQEIISAFEQKKL
                     PIVFNTMQPGRLSTALNWLITLLTLGVIAFVGWRLYASMSGGHGDFELSDASGSQQTT
                     TFADVAGVDEAKNELAETIEFLRDPLRFGRLGGRAPRGILLSGPPGTGKTLLARAAAT
                     EANVPFLSVSGSSFQEKFAGLGAARVRRLFKQARKLSPCVVFIDEIDALGRRRGRSGD
                     SASADQDQTLNQLLIEMDGFQQLNGVVVIASTNRPDILDSALTRPGRFDREIAVNLAD
                     VRGREQILRVHARKLTLAPELELSWIARGTPGFSGADLANLLNEAAIAATRHDAASIC
                     REHVEYARDKILMGAERRGFMMDEEERYATAVHEAGHVAVGLDVKHGDPIHKVSILPR
                     GRALGVTQSLPERDRLMKKREYLEDQIALLLGGRAAEQILLETMTAGASNDIERAVEI
                     ARKMVSEFGMSPLGPIHLGKPEDPHSQALLDRVEHATNEIINAQMARACEIVDARREE
                     IMQLVAGLMERDTLEADEIQHCFGIKSASRAA"
     gene            2688..2834
                     /locus_tag="VGB76_03455"
     CDS             2688..2834
                     /locus_tag="VGB76_03455"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEY0097991.1"
                     /translation="MLWTILVVLLVLWLLGLIGGIGGGLIHLLLVVALIVLVVNLLTG
                     RRTV"
     gene            complement(2934..4709)
                     /locus_tag="VGB76_03460"
     CDS             complement(2934..4709)
                     /locus_tag="VGB76_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY0097992.1"
                     /translation="MTTESNAQSQPPPVPPDWQTHAERTDYRETPRYEETLAYCRKLV
                     AASSSQIRLSDFGRSGEGRALPLVIAATNGTFTPEASRRAGKVVVFVQANIHAGETDG
                     KDAGLALLRDIVVLKTHPRLLEHVTLLFVPIYNVDGHERRSPHNRINQNGPEEMGWRA
                     NATNLNLNRDYMKADAPETRAWLRLWNEWNPELFIDCHVTDGADFRYNVTYQFEQHEN
                     VPQPLRDWMKAAFDGRIVPATEALGNLLTTYMEFRDNRDPVKAGVEGFIGTPRFATGY
                     APLRNRPALLIETHMLKDYRTRVRATYDLLRTALEDVNRDPAGWIRVAKETDQKVVEE
                     GSAYDPERKVALRIAFTDKSVPRVIKGVEFRRELSDVSGAVRVVYGTKPLDYTVPFFN
                     ESYATISVPTPLYYIIPPQWTEVIEVLTAHGVRLQRLAEPLTVEVESYRFSEVKWASA
                     SFELRVPVTQKNRLVREQRTFVAGSVVVPMGQAAGRLVINLLEPDAPDSLLYWGFFNS
                     IFEQKEYGEGYMLEKLAREMLAKDEKLREEFERRVATEPQFAASARARLNFFYERSPY
                     YDKQMNLYPVGRITTPLKARLVDFR"
     gene            complement(4918..5088)
                     /locus_tag="VGB76_03465"
     CDS             complement(4918..5088)
                     /locus_tag="VGB76_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097993.1"
                     /translation="MKDTLLSVAVFLGILLVSALITNWFARTMYNRCASCGTLNAKRR
                     AHCRSCGNALAR"
     gene            complement(5085..>5269)
                     /locus_tag="VGB76_03470"
     CDS             complement(5085..>5269)
                     /locus_tag="VGB76_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0097994.1"
                     /translation="PLYRRGFEAALHPQLRGASYAESAERLRERYSDAYTHDAFRRGY
                     ERGQSYDQNLRERYKS"
BASE COUNT         1043 a         1499 c         1615 g         1112 t
ORIGIN      
        1 ggataagtca ttgccaaatc agaggaacgt ccgggagtta aaaacaggat gtgaagtcga
       61 atggccttcg caagcaaaaa ttacaggaga tgcgacggac aatcaagccg tgagaatgtt
      121 tttcggattg ccatgcgcga acgtggaaac tcgaagagag acgccggaag tcggagggct
      181 tcaggagaaa agcggaaggc agccgaagaa cttctcgatg ttcctcgctg ccttccgctt
      241 ttactttttt tcgcttcagc ttttacgccg cgcggctcgc ggacttgatg ccgaagcagt
      301 gttgtatctc gtcggcttcc agcgtgtcgc gctccatcaa tccggcgacg agttgcatga
      361 tctcttcgcg gcgtgcgtcc acgatctcac aggcgcgtgc catctgcgcg ttgatgatct
      421 cgttcgtcgc gtgctccaca cggtcgagca gagcctgact gtgcgggtct tcaggtttgc
      481 cgagatgaat cggacccagc ggactcatgc cgaactccga aaccatctta cgcgcgatct
      541 ctacggcgcg ttcgatgtcg ttcgacgcgc ccgcagtcat cgtctccaac aagatttgtt
      601 cggcggcgcg tccgcctaaa agcagcgcga tttgatcttc gagatactcc cgcttcttca
      661 tcaggcggtc gcgttcgggc agagactgtg tgacgccaag ggcacgcccg cgcggcagga
      721 tcgagacttt gtgaatcggg tcgccatgct tcacgtcgag gccgacggcg acgtgtcccg
      781 cttcgtgaac ggctgtggcg tagcgttcct cttcgtccat catgaagccg cgacgttccg
      841 cgcccatcag gattttgtcg cgcgcatatt cgacgtgctc gcggcagatt gaggctgcat
      901 cgtgacgggt ggcagcgatg gcggcttcgt tgagaaggtt ggcgaggtct gcgccggaga
      961 aaccgggcgt gccgcgcgcg atccaactga gttcgagttc gggcgcaagc gtcaacttgc
     1021 gcgcgtgcac gcgcaggatc tgttcgcgcc cgcgcacgtc cgcgaggttg actgcgatct
     1081 cgcggtcgaa cctgccgggg cgtgtcaacg cgctgtcgag aatgtccggg cggttggtcg
     1141 aagcgatgac gaccacgccg ttcagttgtt ggaaaccgtc catctcgatg agaagttggt
     1201 tgagagtctg atcctgatcg gcggaggcgg aatcgccgct gcgaccgcga cgacgaccga
     1261 gcgcgtcgat ctcgtcaatg aagacgacgc agggcgagag tttgcgcgct tgcttgaaca
     1321 ggcgacggac gcgagccgcg ccgagtccgg cgaacttctc ctgaaagctc gaaccggaga
     1381 cggataagaa cgggacgttg gcttccgtgg ctgccgcgcg tgcgagcaga gttttgcctg
     1441 tgccgggagg gccggagagg aggatgccgc gcggcgcgcg cccgccaaga cgcccaaagc
     1501 gcagcgggtc gcgtaagaat tcgatggttt cggccaactc gtttttggct tcgtccacac
     1561 ccgcgacatc cgcgaaggtc gtggtctgct gcgaaccact cgcatcgctc aactcgaaat
     1621 cgccgtgtcc gccgctcatg ctcgcgtaga gacgccagcc gacgaaagcg atcacgccga
     1681 gcgtgagcag cgtgatgagc cagttcaagg ccgtcgagag acgccccggc tgcatcgtgt
     1741 tgaagacgat gggaagtttc ttctgttcaa acgcgctgat gatctcttgc tgcgccacgg
     1801 cgtttgtgac cacggcctgt gagcgcgtgc cgtccgtctt ctcgaccacc aacaattcac
     1861 cttccacgag cagcgatctg acggcggcgt cgttcgtcgt cagcgcatgc agttccgtgt
     1921 agttgaccgt ctgcacgacg gggcgactgg agaagtagaa agtttggaag gcgatgacgg
     1981 ccagcagtgt ggtgaccgcg ccgatggcga cgagcaaact cgtcttttgc gtgcggggga
     2041 gcaggcgtga aaaaggcatt aaaatttctc caaacacagc gaggtcgcgt ttctgagggg
     2101 aaacgagcca cgctgtttcg agctatcaag aatctaaaaa attttaactg aggaaacgaa
     2161 cccggttgag aaggccaacg cgcggaattt gcggtaggag cctatctgct gctctgtaat
     2221 tacgcggcgg tagcggccac gaaactaaaa actactgtaa cacccgctct cgctcgaagt
     2281 ccagccgaat gtttcttatt tgtgtcagac gcgttgtttg ataactctca aactaaacgt
     2341 gagcgaagcg accgtcggaa cgtcctcatt gctccgatga caaaaaaaac aggtgaggtt
     2401 gccgccaaag gcgtcgaaga gatgatattt caacactttg ctctgattta gaacaccgtc
     2461 gaaggttcgc aagcggcgta ttttcataca agcgaggcgc ggttaacgtc gccgtttccc
     2521 gtcaaataga cacgctcaag cgttcaaacg tatccatctc aaggctttaa gttgcaggcg
     2581 cgaagacgcg gctaaggttg gcatcacgtt tgcactagag agtgcaggcg ggttcacaag
     2641 aaccgggaga tttgacatca accttttttg ctaaggagaa tgcgaacatg ttgtggacga
     2701 ttctggtagt gcttctggtg ttgtggttac tcgggctgat cggcggtatc ggcggcgggc
     2761 ttatccactt acttttggtc gtggctctta tcgtgctggt ggtcaacctg ttgaccggca
     2821 gacgcacggt ttagtctgga cgacacggac agctcttaaa acttgggaaa agggatgcgg
     2881 tcttaattga accgcatccc ttttcgtttt ccggcaggcg acgcgaaagc gacttaacga
     2941 aaatccacga gccgagcctt gagcggggtc gtgatgcgcc cgaccgggta gaggttcatc
     3001 tgcttgtcat aataaggcga gcgttcgtag aagaagttca ggcgggcgcg cgcgctggcg
     3061 gcgaactgcg gttctgtcgc cacgcgccgc tcaaactctt ctcgcagttt ttcatccttc
     3121 gccagcatct cacgcgccag tttttccagc atatatcctt cgccgtactc tttctgttcg
     3181 aaaatggagt tgaagaaacc ccagtagagc agcgagtcgg gcgcatcggg ttcgagcagg
     3241 ttgatgacga gacgccccgc cgcctgcccc atcgggacga cgaccgaacc cgcaacgaag
     3301 gttctctgtt cgcgcaccag acggttcttc tgcgttacgg gcacgcgaag ctcaaacgac
     3361 gcgctcgccc atttcacctc cgagaagcga tagctctcca cttcgaccgt taaaggctcg
     3421 gccagacgtt gcaggcggac gccgtgcgcc gtgagcactt caatcacctc cgtccactgc
     3481 ggtggaatga tgtagtagag cggcgtcggc acggagatgg tggcgtagct ttcgttgaag
     3541 aagggcacgg tgtaatccag aggcttcgtc ccgtagacga cgcgcaccgc gcccgaaaca
     3601 tcgctcaact cgcgcctgaa ctccacccct ttgatgacgc gcggcacaga cttgtccgtg
     3661 aacgctatcc tgagcgcgac tttccgctcc gggtcgtacg cgcttccctc ttcgacgact
     3721 ttttgatccg tctctttcgc cacacgtatc cagcccgccg gatcgcggtt cacgtcttcc
     3781 agagccgtgc gcagcaggtc gtaggtcgcg cgcacgcgcg tgcggtagtc cttgagcatg
     3841 tgcgtctcga tgagcagggc tggacgattg cgcagagggg cgtagccggt ggcgaagcgc
     3901 ggcgtgccga tgaatccctc gacaccggct ttgaccggat cgcggttgtc gcgaaattcc
     3961 atgtaggtcg tgagcaggtt gccgagcgct tcggtggccg gaacgatgcg cccatcgaag
     4021 gcggctttca tccaatcgcg gagcggctgc gggacgtttt cgtgctgctc gaactgatac
     4081 gtcacgttgt agcgaaagtc tgcgccgtcc gtgacgtggc aatctataaa caactccggg
     4141 ttccactcgt tccacaggcg cagccaggcg cgcgtctccg gcgcgtcggc cttcatgtag
     4201 tcgcgattca gattgagatt ggtggcgttt gcgcgccagc ccatctcttc gggaccattt
     4261 tgattgatgc gattgtgcgg gctgcgccgc tcgtggccgt caacattgta gatcggcacg
     4321 aagagcagcg tgacgtgttc gagcaggcgc gggtgcgtct tcaagaccac gatgtcgcgc
     4381 aggagcgcaa gccccgcgtc cttgccgtca gtctcaccgg catgaatgtt ggcctgcacg
     4441 aagacgacga ccttgcccgc gcgccgcgac gcttcgggcg tgaacgttcc gttcgtggcg
     4501 gcgatgacga gcggcagcgc gcgcccttcg ccgctccgac cgaaatcact cagacgaatc
     4561 tgcgaagacg aagcggcgac gagtttgcga cagtaggcga gcgtctcttc gtagcgcggc
     4621 gtttcgcgat aatcggttcg ctccgcgtgc gtctgccaat caggcggcac gggaggcggt
     4681 tggctttgcg cgtttgattc ggtagtcata atgattaaca gaaacagcag gccacagagg
     4741 ccactcgaac ggtgcatccg gtcaactcca aatcctgaga gcttatttca aaatcgctgc
     4801 ccctcttcct gtgcccctcc ggcgtgcgac tgatctacac gtcagggcgc aaaggaaaac
     4861 ttgaagcgtc tagcggctgg cagtagtttt gcaatgcact cttaagtccc gtttcagtca
     4921 tcgcgcgaga gcattgccgc aggagcgaca gtgcgcgcgt cgcttcgcgt tgagggtgcc
     4981 gcacgaggcg cagcgattgt acatcgtgcg tgcgaaccaa tttgtgatga gcgcgctcac
     5041 gagaagaatc cccagaaaca cggcgacgct caacagcgtg tctttcaact cttgtatctc
     5101 tcccgcagat tttggtcgta gctttggccg cgctcgtagc cgcgcctgaa ggcgtcatgg
     5161 gtgtaggcgt cgctgtatct ctcacgcaga cgctccgccg actctgcgta agacgcgccg
     5221 cgcagttgcg ggtgcagggc ggcctcgaag ccgcgtcgat agagaggct
//
LOCUS       DASWNS010000103         6011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_186557, whole genome shotgun sequence.
ACCESSION   DASWNS010000103 DASWNS010000000
VERSION     DASWNS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186557"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VGB76_03475"
     CDS             complement(<1..1088)
                     /locus_tag="VGB76_03475"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0097995.1"
                     /translation="MRDIISCRQRWPFLIALGFLLTLNCAAQTHLDTKALAAEADKYL
                     SGLVTQNRFGGAILVARDGKVLLRKAYAMGDIEDDVPNSVQTKFPIGSITKQFTAVAI
                     LMLQERGKLSVQDSICKYIADCPATWQPITIEHLLTHTSGLPDSTTFRRPETVGTSPA
                     ANLVELFKKREMVLKPGEKFAFSNPGYIFLGHIIEKASGKSYETFLRENIFEPLKLTN
                     TGYNKPIEILKHRASGYVLRGTQYVNAPFFDIATHHAASALYSTVDDLYVWEQSFYTN
                     KLLSQKSLDEMLKPRQGAYGYGVFIEDRFNLKRMSHAGDLIGFSSYIARYPAQKAVVI
                     VLANYAGTNPLEVGGKLTNIYLRDQMVAP"
     gene            complement(1270..2904)
                     /locus_tag="VGB76_03480"
     CDS             complement(1270..2904)
                     /locus_tag="VGB76_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HEY0097996.1"
                     /translation="MSFLLRCSPKIVIVAVVAGLITGMSNGAILALINVVLSGANSQP
                     RTLVWNFIGLCALMLTSRVVSEILLQRLSQDAIFNLRRELTQQILAAPLRQLEEQGVP
                     RLLNSITGDVPSIAQALNTIAVLCMQIAIVLSILIYMSWLSLTLLACVLVFMALGIAS
                     YQLLMRKGLSELRLVREHWDQLYTHFTALTRGIKELKLHQPRRESFQSELLDPAAKAV
                     RKHNVQGMSILIVATSWGQLLFFVLIGVLIFALPAWQNVSTQALTGYTITILYMITPL
                     QTILGLLPELGRANIATQKVQALGLSLATGIKEQPLKSADSVEQFWDSLKLEGVTHAY
                     HSEKENSNFTLGPIDLSFTPGELVFLVGGNGSGKTTLAKLLTGLYIPEGGVIRLNGQE
                     VTDENRERYRQLFTVVFSDFYLFDSLLGLPTAQLDKQIPALLEQLQLQHKVQIEGGQL
                     STIDLSQGQRKRLALLTAYLEDRQIYIFDEWAADQDPLFKEIFYYRLLPGLQQRGKTI
                     IVISHDDKYYHIADRIIKLDYGKVESAQPVKDLQYT"
     gene            complement(3047..>6011)
                     /locus_tag="VGB76_03485"
     CDS             complement(3047..>6011)
                     /locus_tag="VGB76_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0097997.1"
                     /translation="ELQPQREMSHSPLFQVMLVLQNAPTQDLALSALEVSGIDGGGEA
                     AKFDLTLTLVETHEGISGALGYNTDLFEAETMARMVEHLRVLLVEIVAQPEERIGRLP
                     MLGSVERRQLLEEWNETGREYAAPYLLHELFEQQVERTPEAIALVSGEEQLSYRQLNG
                     RANQLAHRLRDEGVRPEVMVGILMERSVEMVVALLGVLKAGGAYLPLDPAYPVERLHF
                     MLDAARVPVVLTKEHLRINLPANDARIICVDSTGEINSQYSRSNPESEIRADNLAYVI
                     YTSGSTGTPKGVMITHGSVVNLIAALKLTVYAKYESPLCVSVNAPLTFDSSVKQIFQL
                     LAGHTIDIIPEEVRRDGEALLQHIRQHRIQVLDITPSQLRMLIEAGLQESVEPLPAIV
                     LVGGEEIDSTLWQTLSADSARAYYNVYGPTECTVDATACPVEPGREMPSLGRPLSNVE
                     IYVLDSHLQPAAIGVAGELHIGGAGLARGYLNRGELTAEKFIPHPFSEKPGARLYRAG
                     DLVRYRVDGSVEFLGRVDEQVKVRGFRIELGEIESVLGRHAQVRECVVIVREMGGDRM
                     LVAYLVTEGEEPLSVNELRGFAQAQLPAYMVPSAFVMLDEMPLTPNGKVDRKSLSATD
                     KGRSALQQGFVAPRDLLELQLAQVWEEVLGVSPIGVRDDFFENGGHSLLAVRLLARTQ
                     QVVGETFALTALFQAPTIERLANLLRRSKRETHWSALVPIQPHGTGTPFFCVHPGGGN
                     VLCYMDLARSLGSGQPFYAFQARGLDGLQTPLTRVEDMAADYLQAMRSVQPDGPYLIG
                     GWSLGGTIAYEMARQLSEQGESIALLALFDTSAPSANLEVAPTDDGTLMLAFAQDLGF
                     SADMAHIDVADFKRLSHDEQIACLWEQAQLANLLTPDMELAQMRNLLRVFEANVRARN
                     AYRESGYNGELTLFRAADGAQHSPQDVSFGWSPWTGREIEVLLTPGSHYTMLKKPHVE
                     KLAERLGERLRQTEN"
BASE COUNT         1317 a         1726 c         1611 g         1357 t
ORIGIN      
        1 ggagcaacca tctgatctct gaggtaaata ttcgtaagct taccgccgac ctccaacgga
       61 ttagtcccgg catagttggc gagtacgatc acgactgctt tctgtgcggg ataacgcgcg
      121 atataactgc tgaagccgat taaatctccg gcatggctca tacgcttcag attaaacctg
      181 tcctcaataa agactccgta accgtaagct ccctgccggg gtttcaacat ttcatccaga
      241 gacttttgtg agaggagctt gttcgtatag aacgactgct cccacacgta gagatcatcc
      301 acggtcgagt atagggcgct ggcggcatgg tgcgtggcaa tgtcgaagaa tggagcgttg
      361 acatactgtg tgccccgcag gacgtaaccg gacgcgcggt gtttaagaat ctcaattggc
      421 ttattgtatc cggtgttcgt taacttcaac ggctcgaaga tattttctct cagaaaggtt
      481 tcataacttt taccggaagc cttttcgatg atgtgaccga gaaatatata gccggggttg
      541 ctgaacgcga atttttcgcc cggcttgagc accatctccc gtttcttaaa gagttcgacc
      601 agattggctg cgggtgaagt tcccaccgtc tccgggcggc ggaacgtggt gctgtccggc
      661 agtccggaag tgtgcgtcaa gaggtgttcg atggttatcg gctgccacgt cgccgggcag
      721 tctgcgatgt acttacatat tgaatcctgc acactcagtt tgccgcgctc ctgtagcatc
      781 aggatggcaa ctgccgtgaa ttgtttcgtg atagagccga tgggaaactt cgtctgcacg
      841 ctgttgggca cgtcgtcttc aatatccccc atcgcgtaag ccttacgtaa gagcaccttg
      901 ccatcccgcg cgacgaggat cgcgccgccg aaacggttct gcgtgacaag accgcttaga
      961 tacttgtcgg cctccgccgc cagcgctttt gtatcgagat gtgtctgcgc tgcacagttc
     1021 agggtcagaa gaaaacctag agctatcagg aacggccacc gctgtctgca agatatgatg
     1081 tctctcatag tggatactcc tttggataag ggatatttga acgcgcttca accatccgga
     1141 agatcgagtc ggcgcaaatt aaacgcctgc cttgctgcgg ttcaatagac ccggcagatg
     1201 gaaccgtgcc gggcgaattc ttcaaggtga gaggatgatc cgtgtgatcg aagtggagcg
     1261 ggttaagggt tatgtgtatt gcaaatcctt cacgggttgg gcagactcca ccttgccgta
     1321 gtccaactta atgatcctgt ctgcgatgtg ataatacttg tcgtcgtggc taatcacgat
     1381 gatggtcttg ccgcgctgtt ggaggccggg caggagccgg tagtagaaga tttctttgaa
     1441 cagggggtct tggtcggcag cccactcgtc gaaaatatag atctgtctat cttccagata
     1501 ggccgtcagc agcgcgagac gtttgcgctg cccctgcgag agatcaatgg tggaaagctg
     1561 cccgccttcg atttgcactt tatgttgcag ttggagttgc tcaagcagcg ccggaatctg
     1621 cttgtcgagt tgtgcggtgg gcaaacccaa caggctgtcg aagagataaa agtcggagaa
     1681 gaccacggtg aaaagctgtc ggtagcgttc tcgattctcg tccgtcactt cctgcccgtt
     1741 caagcggatg acgccgccct cgggaatgta taaacctgtg agcagtttgg cgagagtcgt
     1801 ctttccactg ccgttgccgc cgacgagaaa cacgagttcg ccgggggtga aactcagatc
     1861 aatcgggccg agcgtgaaat tgctgttctc tttctcgcta tgataggcat gcgtcacgcc
     1921 ctcaagtttg aggctatccc agaactgctc aacgctatcg gcggatttca acggctgctc
     1981 ctttatgccg gttgccagtg aaagcccgag cgcttgtacc ttttgcgtcg caatattggc
     2041 gcggccaagt tccggcagca gacccagaat cgtctgcaag ggagtgatca tgtaaagaat
     2101 agtgatggtg tagccggtca acgcctgtgt gctgacgttc tgccaggcgg gcaacgcgaa
     2161 gatcagcacg ccgatcagca cgaagaacaa cagttgaccc caactcgtgg cgacaatcag
     2221 gatgctcatg ccctgcacgt tatgcttgcg cacggccttc gcagccgggt cgagcaactc
     2281 cgattgaaaa gattcccggc gcggctggtg taacttgagt tccttgatgc ctctcgtgag
     2341 cgcggtgaaa tgtgtataga gctggtccca gtgttcgcgc accaacctga gttcgctcag
     2401 accctttctc atcaacaact ggtaactggc gatgcccagc gccatgaaga cgagcacgca
     2461 ggccagcagc gtgagggaga gccaactcat gtaaatgagg atgctgagga cgatggcaat
     2521 ctgcatgcac aggacggcga ttgtattgag cgcctgcgcg atgctcggca cgtcgcccgt
     2581 gatgctgttc aggagacgcg gcacgccctg ttcttccagt tggcgcaaag gcgcggccag
     2641 aatctgttgc gtgagttctc tacgcaaatt gaagatggca tcctgcgaca gacgctgtaa
     2701 taagatttcg gacacgactc tgctggtgag catcagcgcg cataatccga taaagttcca
     2761 gaccaatgtt cgtggttgag agtttgcgcc gctcagtacg acgttgatca gagccagaat
     2821 agcgccgttg ctcatgcctg tgattaatcc cgcgaccact gcgacaatca caattttcgg
     2881 agagcagcgc agaagaaagg acattaattt caaagtgttt accgcctttc gactttcaga
     2941 gacaggtgaa tcaggagtta aaagctaaca aatagaactc ggtgtattta agattcgttt
     3001 gccagcctgt ttgagtgcag ggcaagaggg gttttgagaa gtggctttaa ttttctgtct
     3061 gccgtaaacg ctcgcctaat cgttcggcca acttttccac gtgaggtttt ttgagcatcg
     3121 tgtaatgact gccgggcgtg agcagaactt caatctcccg cccggtccat ggcgaccacc
     3181 cgaagctcac atcctgcggt gaatgctgcg cgccatcggc ggctctgaag agcgtgagtt
     3241 cgccgttata gccggactct cgataagcgt ttctagccct gacgttggcc tcgaagacac
     3301 gcaacaggtt tcgcatctgc gcgagttcca tgtcgggagt caacaggttg gcaagctgcg
     3361 cctgctccca caggcatgcg atctgttcgt cgtggctgag gcgtttgaaa tccgccacgt
     3421 ctatgtgagc catgtcggct gagaagccga ggtcttgggc gaaggcgagc atcagcgtgc
     3481 catcatccgt cggagcgact tccagattcg cagatggcgc gctggtatcg aacagagcaa
     3541 ggagcgcgat gctctcaccc tgctcggaga gttgccgcgc catctcgtag gcaatcgtgc
     3601 cgcccagcga ccagccaccg ataagataag gcccatcggg ctgcacgctg cgcatggctt
     3661 gtagatagtc ggcagccatg tcttcaacgc gcgtcagggg cgtctggagg ccgtcgagtc
     3721 cccgcgcctg aaaagcgtag aagggctggc cggagccgag gctgcgcgcc aaatccatat
     3781 agcagagaac atttcccccg cccggatgaa cgcagaagaa aggcgttccg gttccgtgtg
     3841 gctgaatagg cacgagcgca gaccagtgcg tctctctctt gctccggcgc aaaagatttg
     3901 ccagacgctc aatcgtaggg gcttggaaca atgctgtgag ggcgaaagtc tctccgacga
     3961 cttgttgagt tcgtgcgagc agacgcacgg ccagcagcga gtgtccgccg ttttcaaaga
     4021 agtcgtcccg cacgccgatg ggagagactc cgagcacctc ttcccaaacc tgtgccaact
     4081 gtagttccag taagtctctc ggcgcgacaa aaccctgttg cagcgcgctc cgccccttat
     4141 cagtcgccga cagagatttc cggtcaacct tgccgttagg cgtcagcggc atctcgtcca
     4201 gcatgacgaa agccgacgga accatgtaag caggtaactg cgcttgcgcg aatccacgta
     4261 gttcattaac ggagagcggc tcttcgccct cagttacgag gtaggccacc aacattctat
     4321 cgccacccat ctccctgact atcaccacgc actctcgcac ctgtgcgtgt ctccccagca
     4381 ccgactcgat ctcacccaat tcgatgcgga agccgcgcac cttcacctgc tcgtctacac
     4441 gccctaaaaa ctcgacgctc ccatcaaccc ggtaacgtac cagatcgccc gcccggtaca
     4501 atctcgctcc cggcttctcg ctgaacggat gcgggataaa tttttccgcc gtcaactcac
     4561 cgcgatttaa ataccctcgc gccaagcccg cgccgccgat gtgtaactcg cccgcaaccc
     4621 ctatcgctgc cggttgcaga tgcgaatcga gcacatagat ttcgacattg ctcaacggtc
     4681 gtccaagact cggcatttca cgtcccggct caaccgggca ggcggtggca tctacggtgc
     4741 attcggtcgg gccataaacg ttatagtaag cccgcgcgct gtctgcacta agagtttgcc
     4801 agagcgtcga gtcgatctct tcgcctccga ccagcactat cgcaggcagt ggctccacac
     4861 tctcctgcaa gcccgcttct atcaacatcc gtaattgaga gggagtgatg tcaagcacct
     4921 gtatacgatg ctgccggata tgttggagca atgcttcgcc atcgcgcctc acctcttcgg
     4981 gaataatgtc gatggtatga ccggccagta gttgaaagat ttgcttgacc gaagagtcga
     5041 aggtcaaagg ggcgttaacg ctcacacaca gcggcgattc atattttgca tagaccgtca
     5101 gtttcagcgc agctatgaga ttaaccaccg aaccgtgagt aatcattact cccttcggtg
     5161 tgcctgtgga gccggaagtg tagattacat aagccagatt atcagccctt atttcactct
     5221 ccgggttgct cctgctgtat tgagagttaa tctcccctgt actatcaaca caaataattc
     5281 gtgcatcatt agccggaaga tttatcctca agtgttcctt agtcaacacg actggaaccc
     5341 gtgcagcatc gagcatgaag tgcagacgct cgaccgggta agccgggtcg agcggcagat
     5401 acgcacctcc cgccttgagc actccgagga gggcaacaac catctccacg gagcgttcca
     5461 tcaggatgcc caccatcacc tccggcctca caccctcatc ccgcagccgg tgcgcaagtt
     5521 gattggctcg cccattcagt tgccggtaac tgagttgttc ctccccagaa accagagcaa
     5581 tggcctccgg cgtacgctcc acctgctgct cgaacagttc atgcaacagg tatggcgcgg
     5641 cgtactccct gcccgtctca ttccactctt ccagcagttg ccgccgctcc acgctcccca
     5701 acatcggcaa ccttccgatg cgctcctccg gctgcgccac tatctccacc aacaacaccc
     5761 gcaagtgctc caccatccgt gccatcgtct ccgcttcaaa taagtctgtg ttgtacccca
     5821 gcgcgccgct aatcccttca tgcgtctcca ccagcgtcaa cgtcaggtca aacttcgccg
     5881 cttccccacc tccgtctatg ccgctcactt ccaacgcact caaagccagg tcttgcgtcg
     5941 gagcgttctg caacacgagc atcacttgaa agagcggcga gtggctcatc tcccgctgcg
     6001 gctgcaactc t
//
LOCUS       DASWNS010000104        15984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_497655, whole genome shotgun sequence.
ACCESSION   DASWNS010000104 DASWNS010000000
VERSION     DASWNS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15984
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497655"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VGB76_03490"
     CDS             <1..560
                     /locus_tag="VGB76_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY0097998.1"
                     /translation="LTIGWNALEAVVSIGAGLFAGSVALVGFGVDSVIESTSGAVLLW
                     RLRDGERSEQRERIALRLVGVSFLLLAAYVAFGAAKSLFMREPPEVSYTGIGIAALSL
                     VVMPLLARAKRRVATQINSRAMKADSRQTDLCAYLSAILLGGLLLNALFGWWWADPLA
                     ALVMSPIIVREGVEALRGETCVDCH"
     gene            662..1357
                     /locus_tag="VGB76_03495"
     CDS             662..1357
                     /locus_tag="VGB76_03495"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEY0097999.1"
                     /translation="MFYTRTGGGRLQRPGSRLLRLSVSFMSDVEGFILTGGASSRMGT
                     EKARLSLGGLTFIERIAGALRAITPDLRAVSARTESFNLNLPVVPDIHHDCGALGGLH
                     AALRACRAPWAAIVSCDLPFVTGELFVRLCELRTQETDAVAPLQTDGRPQPLCALYSP
                     AGCLERVERLLQEGERRPRALLRQVRTRWVAHTELDDLRDAGLFFMNVNTPEDYSSAL
                     ERVGESRSDEDKG"
     gene            1527..2483
                     /locus_tag="VGB76_03500"
     CDS             1527..2483
                     /locus_tag="VGB76_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098000.1"
                     /translation="MLKALLPVALLLVLGVGAGLFYIVRTIAHPVPHPYLVTPETFPA
                     YSARGLRVNEEHWSNSDGTQARGWLLRGAEGAPAVILLHRYGADRSWLLNLGVKINEA
                     TNCTILWPDLRGHGENPIVPQTSFGARESADVAAAVAFLRNLKTAQKRPLVAERFGFF
                     GVELGAYAALAASARAPENALVRVLVLDSVPASADELLANAIRDRMGFDNQLLNLFTR
                     GAARLYFLGGYENDSACALAAQLVNKRVLLLSGEDAGTLRASTKSLAECLPTPASVAL
                     HADLPVTGFTASSATGEQGETYDRLVIEFFSKAFNEESQTAR"
     gene            complement(2500..4119)
                     /locus_tag="VGB76_03505"
     CDS             complement(2500..4119)
                     /locus_tag="VGB76_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY0098001.1"
                     /translation="MLAKRGWLLLLLTTAVVYFYGLGRAPFVGADEPRYAQVAREMHL
                     RGDAVTPTLGGHTWFEKPALTYWAAMAGYSLFGVSEWSARLGVACAGWLTVLLVGWTA
                     KRVEEAEGARASWLQLSTGAVAATSAGLIVFSRGVNFDIFITMTVACALASFFAAETE
                     TSARRKRWLLAGFYAGMGAALLAKGLIGVVLPAGVVGVYYVLRRRRPDVWRSAFWGLL
                     ITAAVAALWYAPVVARHGWTFIDEFFVKHHFARYLSNRYHHPQPFWFYFTILPALMLP
                     WSAFLASAIYRARRWTWGSDDALSRLRLFALAWVLFPLLFFSVSGSKLPGYILPALPG
                     AALLAGERLAGFLRGEGGLRTLRATALLIFALAVAGILYALRLNELSRLCIALVGAPV
                     VAASAYVLLAGRRRVWSVLSVVGVSFLTTLLVAGCALDRVSARESVRDLLRAAERRGY
                     GDAPVLNLHTVERTGEFYAAGRLIYNEENGEPLKFEGAGEVLDVLRARGGTVLVIVPP
                     EYAAQLFNFQPLQTEMIGDNGQVALVAARLR"
     gene            4350..5369
                     /locus_tag="VGB76_03510"
     CDS             4350..5369
                     /locus_tag="VGB76_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0098002.1"
                     /translation="MLLRTAAIGTGHLGRQHARLHAALAAEGRTQFVAVCDADEATAR
                     RVASERETEWTTDWRSLLGRVDAVSLAVPTEAHAEIACPLLEAGIHVLVEKPISHTLA
                     EADQMIASASRGRAVLMVGHVERFNPALTALYPHVRSPLYFEIHRVGEFTARSLDIDV
                     VLDLMIHDLDVVQWLVGSDVEVTDVRAVGIPVLTERVDASNARIEFASGAVANITASR
                     VGMEKIRKMRFFQPHDYIAVDYAARHTSISSLAPPDPSSARPGVQLRRLEIADVEPLR
                     AEIEAFLEAASTGQPPPISGADGRRALSLALRVIERINEHTTNAGLHALAAATRSTFK
                     TPGDT"
     gene            5386..6552
                     /locus_tag="VGB76_03515"
     CDS             5386..6552
                     /locus_tag="VGB76_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0098003.1"
                     /translation="MISNRTHEVSIDALLAGRIAAGDFPSAVYLVAERERVAFANALG
                     DAVREPVRHAATVETIYDLASLTKPLVTGLLCARRVERGELEMDRAVASYLPEFAPEG
                     DKASITLRHLLTHTSGLPAWQPLRFTSHNRRERALATIAATALSYPTGSRVVYSDLGF
                     ITLGLLLEKISGAPLDELAQSEIFQPLQLQRTFFRPEQAYLTGVAACELSGNAYERGM
                     CEAEGQAESAGAAGAWREELIWGEVHDGNAYFLGGAAGHAGLFSNAHETFRLAEQFIA
                     RRTQLLAPETCELFRTNLTPDLNEARSFAWQLAATPDSTAGDALAPDCFGHLGFTGTS
                     CWIDALRERVFILLTNRTHTRTLPFVNINGVRRRFHTLAVHALGDAGSFVNHPQ"
     gene            6549..8000
                     /locus_tag="VGB76_03520"
     CDS             6549..8000
                     /locus_tag="VGB76_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY0098004.1"
                     /translation="MKPLDETSRHESALSPEHVKAAAGRVSFGEAFRFWVKLGFISFG
                     GPAGQIAIMHRELVERRRWLSEERFLHALNYCMLLPGPEAQQLAIYIGWLMHRTWGGI
                     VAGAFFVIPSIFILLALSYVYAKYGQLTSIAGVVNGFKPVVVAIVVEALLKIGGRALK
                     RRIHFLIAVAAFAAIFFLHIPFPVIVLAAGLLGLAGARFRPDVFAPPAPKVSKSAQPS
                     KSKETREETARREVSHDAQARAAFVIADDAPPPAHTLPSSGRTLRILLAGIVLWVLPF
                     AAIIWWRGWGSLHAEEYRFFTQAALVTFGGAYAVLAYVTQAAVGSFNWLTHAQAVDGL
                     ALAETTPGPLIMVLQFVGFMAGWNHPPQGLDQTTSAIVGALVTTYATFLPCFIFIFLG
                     APYIEILRGNKNLTSALSGITAAVVGVILNLALVFGAAVIWPHGLSGGANLFAAIMSV
                     AAFVALYRLKLDVLWVVLAGGALGFLHGWFFAR"
     gene            8148..10634
                     /locus_tag="VGB76_03525"
     CDS             8148..10634
                     /locus_tag="VGB76_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY0098005.1"
                     /translation="MPILPFKFTKLIALSVALALLAQSFVSAQDAATQTRPRRVQTET
                     PPVSTIQPPRVDVAVTRPGAEPNIRIGLSTNARSVTISTSGQALTVLRTQPGEMHAPA
                     ALAVSRVRIEPRMLMPLPAPRADENGLFRVEVAAVTSEAQAASIARDVKELTGEAPEV
                     VRDAATTWRVRVGLPTPRTEAEELRTRLEEAGIASVSLVGVQRPAGTTPQPQTEAARA
                     RENSNAPTSAKRTQNSSTAHRSESTGVRLATRATLPTRGLVVSAGGTSPLLDSRAPVT
                     FAPTEASAPLRFNEKPYRGQLEVFTNLNGSLTVVNVLGLEEYVRGVVPNELSPGGYGA
                     LEALKAQAVAARTYAVSNRGQFNAAGFDLLPTIRSQVYGGLSTEHPLSTRAVEETRGL
                     IATYRGEPINALYTSTCGGRTEHAENIFGGEFVPYLRARECTAHAAHASLVSTNIQTT
                     REPHNLRDALHAASAREGALLAVHGFRLPSRINDEWLAAAVSAEEVRALLEVAARLAR
                     RPAPLASINNDATRPPGFSTALALALDGESRADVLLSADDVNYLLAFRDAEEIPAQNR
                     ADVALLLRDGHLTLHTDATLRPRQPMSRARAMHTVIHLLEARGLLQLQKATARPPVGG
                     ALVVRAGKSPERAIAVAPDAYLFRTFGEQLFQMRNVNVVGGEPVAYHTDARGAVDYLE
                     LQTAPKGASAERVSPYTTWTTTLSAGEVAHRLARSAGRIGSLLDLRVASRGASRRALD
                     LEVTGTTGTAHVRGGRIRSALGLREQLFVIDRKYDESGRVASFVFTGRGWGHGVGMCQ
                     VGAYGMARAGLRYDQILKNYYTDIALTKMY"
     gene            10661..10924
                     /locus_tag="VGB76_03530"
     CDS             10661..10924
                     /locus_tag="VGB76_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY0098006.1"
                     /translation="MKETETGSAVTLFVVPNCPLCTHARAWLAQHEITFVERDVKRDF
                     GALRDMYRLTGQNLVPVFARGEHALVRPTDKQLTEFLVNRQRT"
     gene            11099..12058
                     /gene="miaA"
                     /locus_tag="VGB76_03535"
     CDS             11099..12058
                     /gene="miaA"
                     /locus_tag="VGB76_03535"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY0098007.1"
                     /translation="MSSLSRHQPIIVVAGPTASGKSELGIALALRFEGEIINCDSVQI
                     YQGIEIATAKVPPAEQRGVPHHLLDFVPPHVNFTAGAWAETATRTLSEIEARGRLAIF
                     VGGTGFYLRALREPFFPSPPTDEALRARLTQLRERRGAEHLHRILRRLDPASASRLHP
                     RDWSRVQRALEVRVQTGAPLSLQQPARPAPPPFAARLHIFALSPPRDALYARINERAA
                     RHFQAGLIEEVSRLLKAGVPAASNALGAHGYRRAVEYLNGARTLESAIEQTKLDVRHY
                     AKRQLTWFRREPGVEWIEGFGDEPETQERVAARVGELLAINAG"
     gene            12228..12530
                     /gene="hfq"
                     /locus_tag="VGB76_03540"
     CDS             12228..12530
                     /gene="hfq"
                     /locus_tag="VGB76_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY0098008.1"
                     /translation="MDSKPSPQNIQDAFLNTVRREKDAVTIYLMNGAKLMGRIKSFDK
                     FSVLLEAGAQEQLIFKHAISTISHARRHASGELRGAHSFEPAGEHNSHDASERADD"
     gene            12572..12964
                     /gene="tmk"
                     /locus_tag="VGB76_03545"
     CDS             12572..12964
                     /gene="tmk"
                     /locus_tag="VGB76_03545"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY0098009.1"
                     /translation="MNGAFITFEGIDGSGKSTQVRMLSSVLRLRGMDVVQTREPGGTP
                     LGSRLREALLDAQEQVDPLAELLLYAADRAQHVRALLLPALAAGRIVLSDRYADATVA
                     YQQLLNAKNILYTVSVCMGGCFAFCFRG"
     gene            13021..13599
                     /locus_tag="VGB76_03550"
     CDS             13021..13599
                     /locus_tag="VGB76_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0098010.1"
                     /translation="MSLGLCGPHRTGKTTLAQALASRTGRPFVPTNVSAVFAECGIDP
                     AAPIDFQTRLRIQNQILDSCEKVWQTDRAPFITDRTPIDLMAYTLGDIQGTTNVNFTE
                     LESYMHNCFEVTNKNFDALIILQPGIPLIKEGGKAALNSAYIEHLNSLIIGLCNDERL
                     NCGVLKIKREMTDLGERVQKILGAGLDVRVLP"
     gene            complement(13616..14851)
                     /locus_tag="VGB76_03555"
     CDS             complement(13616..14851)
                     /locus_tag="VGB76_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEY0098011.1"
                     /translation="MPPIRPELLDELLKDYQMPEDLLGQDGLLQQLTKALVERALDGE
                     LTHHLGYEKHDSAGDNSGNSRNGTTPKTMRGKRGQVQIDVPRDHTSDFKPQLVKKGQT
                     RFDGLDEKIISLYSRGMTQREIQGHLEEIYGVEISPSLISTVTDAVLDEVRTWQARPL
                     DSVYPILYLDALQVKVKSHGRVTNKSIYLAFGVNLQGLKEVLGMWAADNEGAKFWMQV
                     VTELKNRGVSDIFIACVGGLKGFPEAIEALYPQTQVQLCMVHLVRHSLSYVLHKDRKA
                     VADDLKTIYQAATLEEAERQLTEFEETWAANYPVIAKSWRSNWARVVPMFGYPSEIRR
                     AVYTTNTIESLNMTLRKVSKNRSLFPNDEAVFKLMYLALRNISKPWTMPIPNWSAAMN
                     QFAILFDGRSPDVEMIESL"
     gene            complement(15089..15790)
                     /locus_tag="VGB76_03560"
     CDS             complement(15089..15790)
                     /locus_tag="VGB76_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098012.1"
                     /translation="MTESRLGLKEKALLAALKCTGGDINKSFTLEDLLVEAWKQDHEA
                     WGLRGYEKVYPDVDRLSKEINSRGKSLGAKGLVNDGFLAKQNSRVYRLTTKGLAFAAR
                     LKPDDETVQVKVTRALHNTIKSIMDNQVFRSWLTNPEQPKYFRDAGYFWGIAPGSPAS
                     TVKLRIGAVDSALKDAYEFLEIHGIDEVADSRSRILFDRKDIERCSEFQLVLKKRFAH
                     ELTILDPEIVNKDKD"
BASE COUNT         3106 a         5107 c         4558 g         3213 t
ORIGIN      
        1 atctcaccat cggctggaac gcgcttgagg ctgtagtttc catcggcgcg ggcttattcg
       61 caggcagcgt cgcgttggtg ggcttcggcg tggactccgt gatcgagtca acgtccggcg
      121 cggtcttgtt gtggcgtctg cgcgacggcg agcgcagcga acagagggaa cgcattgcgc
      181 tgcggctcgt cggcgtcagc tttcttctcc tcgccgctta cgtggctttc ggcgcggcaa
      241 aatctctctt tatgcgcgag ccgccggaag tgagttatac gggcatcggc atcgccgcct
      301 tgtctctcgt cgtgatgccg ctcttagcca gagcaaaacg gcgcgttgcc acgcagatta
      361 acagccgcgc gatgaaagcc gactcgcggc agacagacct ttgcgcttac ctgtccgcga
      421 tccttctcgg cgggcttctt ttaaatgcac tgttcggctg gtggtgggct gacccgttgg
      481 ccgcgctcgt catgtcgccg attattgtca gggaaggcgt agaggctctg cgcggcgaga
      541 cgtgcgttga ttgccactaa tcaacgcaca ggtctttcaa cttgatctgc cttgctgcac
      601 gctcgcgccg cgtgttaatt ttcgcgggcg atgctacctt tatcctcccg acacaagcct
      661 tttgttttac actcgcaccg gcggcgggcg tctccaacgt cccggctcgc gattgctgcg
      721 cctgagcgta tccttcatgt ctgacgtgga gggttttata ttgacgggcg gcgcgtcgag
      781 ccggatggga accgagaagg cgcgcctctc gcttggcggg ctgactttta tcgaacgcat
      841 cgccggagct ttaagggcga ttacgcccga cctgcgcgcg gtgagcgcgc ggacggagag
      901 tttcaattta aatctgccgg tcgttccaga catacatcac gactgcggcg cgctcggcgg
      961 cctgcacgcc gcgttgcgcg cgtgtcgtgc gccgtgggcg gcgattgtct cctgcgatct
     1021 gccgttcgtt acgggcgaac tgttcgtgcg gctctgcgaa cttcgcacgc aggagactga
     1081 cgccgtcgcc ccgctgcaaa ctgacggtcg cccgcaaccg ctctgcgcgc tctactcgcc
     1141 cgcaggttgt ctggaacgcg tcgaacgact tttgcaggag ggcgagcgac gcccgcgcgc
     1201 tttgctccgg caggtgcgca cgcgctgggt cgcgcacacg gagttggatg atctacgcga
     1261 cgccgggttg ttcttcatga acgtcaacac gccggaagat tacagcagcg cgcttgagcg
     1321 cgtaggagaa agtagaagcg atgaagataa aggatgaacg atgaagtgga gacagaattc
     1381 agaattcggg agccggtagt aaagtcagcc ggctttgctt ccgctccctg tgttctgaag
     1441 tctgtttgct gtcttccgat tcatccttca tccttcatcc ttcatcctta gattaaatgt
     1501 cgcgccgtgg aagaccggga cgaagattgt taaaagcgct gctgccggtg gcgttgctgc
     1561 tggtgttggg cgtcggcgcg ggtctgttct acatcgtccg cacgattgcg catccggttc
     1621 cgcatcctta tctcgtcacg ccggaaactt ttcccgctta cagcgcgcgc ggcctgcgcg
     1681 tgaacgaaga gcattggagc aactccgacg ggacacaggc gcgcggctgg ctgctgcgcg
     1741 gcgcggaagg cgcacccgcc gtcatactcc tgcatcgcta cggcgcagac cgttcatggc
     1801 tgctcaacct cggcgtcaag atcaacgaag ccaccaactg cacgattctc tggcctgacc
     1861 tgcgcggcca cggcgagaac cccatcgtcc cgcaaacctc tttcggcgcg cgtgagagcg
     1921 cggacgtggc ggcggcggtg gcgttcctgc gcaatctcaa gaccgcacag aaacggccac
     1981 tcgtcgccga gagattcggc ttcttcggcg tcgaactggg agcgtatgcc gcgctcgccg
     2041 cctccgcacg cgcgccggag aacgcgttgg tgcgcgtgct cgtcctcgac tccgttcccg
     2101 cctctgccga tgaactgctc gccaacgcca tccgcgaccg catgggcttc gacaaccaac
     2161 tgctcaatct gttcacgcgc ggcgcggcgc gactctattt tctcggtggt tatgaaaacg
     2221 actccgcgtg cgcgctcgcc gcgcaactcg tgaacaagcg tgtgctgctg ctgagcggcg
     2281 aagacgcggg cactctgcgc gcgtccacca aatcgctcgc agagtgcctg cccacgcccg
     2341 cctccgtcgc gctccacgca gacctccccg tgacaggttt caccgcctcc tccgccacgg
     2401 gcgaacaggg cgaaacctac gaccgcctcg tcatcgaatt cttctccaaa gccttcaacg
     2461 aagagtcgca gaccgcacgc taacggcgcg cccgtcgcgt caacgcaggc gcgccgcgac
     2521 gagcgcgacc tgcccgttat cgccgatcat ttcggtctgt agaggttgga agttgaacaa
     2581 ctgcgcggcg tattccggag gcacgatgac gagcaccgtg cccccgcgcg ccctcaacac
     2641 gtcgagcact tcgcccgcgc cctcaaactt caaaggctcg ccgttctcct cgttgtaaat
     2701 cagacgaccc gccgcataaa actcgcccgt gcgctccacc gtatgcaggt tcaagaccgg
     2761 cgcgtcgccg taaccgcgcc tctccgccgc acgcagcaga tcgcgcacag actcccgcgc
     2821 agacactcta tccagagcgc aacctgcgac cagcagtgtt gtcaaaaacg aaacgccgac
     2881 gacagacaag acgctccaca cgcgtcttcg ccctgccaac agaacgtatg cgctcgcggc
     2941 gacgaccggc gcgccgacaa gcgcgatgca gagtctcgaa agctcgttca atctcagtgc
     3001 atacaaaatt cctgcgaccg cgagcgcgaa gatcaacagg gcggtcgcgc gtagcgtccg
     3061 caagccgccc tcgccgcgca aaaaacctgc gagccgttcg cccgcgagta gagccgcgcc
     3121 cggcagcgcg ggcagaatgt agcccggcaa cttcgagccg gacaccgaaa aaaagagcag
     3181 cggaaacaag acccacgcga gcgcaaacag gcgcagcctg ctcaaagcgt cgtcgctgcc
     3241 ccacgtccag cgtcgcgctc gatagatggc cgaggcgaga aacgcgctcc acggcagcat
     3301 gagcgcgggg aggatcgtaa agtaaaacca gaagggctgc gggtggtgat acctgttcga
     3361 gaggtagcgc gcgaagtgat gcttgacgaa gaactcgtct ataaaagtcc agccgtgccg
     3421 cgcgacgacc ggcgcatacc acaacgcggc gaccgccgcc gtgatcaaca gaccccagaa
     3481 cgcagacctc cacacgtcag gccgacgccg ccgcaatacg taataaactc cgaccacgcc
     3541 cgctggaagc acgacgccga ttaaaccttt tgccaagagc gccgcgccca tccccgcgta
     3601 aaatcccgcg agcagccaac gctttctgcg cgccgatgtc tccgtctccg ccgcgaaaaa
     3661 agatgcgagc gcgcacgcga ccgtcatcgt gatgaagatg tcgaagttca ccccgcgcga
     3721 aaagacgata agccccgcgc tcgtcgccgc caccgcgccc gtcgagagtt gcaaccaact
     3781 cgcacgcgcg ccctcagcct cctccacgcg cttcgccgtc cagcccacca gcaacacggt
     3841 cagccaaccg gcgcaggcca cgccaagccg cgccgaccat tccgagacgc cgaacagact
     3901 gtaacccgcc atcgccgccc aataggtgag cgcgggcttt tcaaaccacg tatggccgcc
     3961 gagcgtgggc gtaaccgcgt cgccgcgcag gtgcatctcg cgcgccacct gcgcgtaacg
     4021 cggttcgtcc gcgccgacga aaggagcgcg ccccaacccg taaaagtaaa ctacggctgt
     4081 cgtcaacaac agcaaaagcc agccgcgctt ggcaagcatc gaaggttgca agtatagtca
     4141 ccgaaaagct gttagccgtt agctattagc cgttagcagg atttttgctt taccgcttga
     4201 gtatcaacga agacgcgccg atgtacaatc tgcgacagct attttgagac tgactgccga
     4261 caaacgatag cgcacaacca acgtttgaaa gccgaaagcc aatcgctgac ggctgaaagc
     4321 taatcgctgt caactgaccg ccaatacaca tgcttctacg caccgccgcc atcggcacag
     4381 gccacctcgg acgccagcac gcgcgcttgc acgccgcgct cgccgcagaa ggacgcacgc
     4441 agtttgtcgc cgtctgcgat gctgacgaag cgacggctcg ccgcgtcgcc tccgaacgcg
     4501 aaaccgaatg gacgaccgac tggcgcagcc tgctcggtcg ggtagacgcc gtgagcctcg
     4561 ccgtgccgac cgaagcgcac gcagagatcg cctgcccgct cctcgaagcg ggcatccatg
     4621 tgctcgtcga aaagcccatc tcgcacacgc tggcggaagc ggatcagatg atcgcctcgg
     4681 cctcgcgcgg tcgcgccgtg ctgatggtcg gccatgtcga acgcttcaac ccggccttaa
     4741 ccgcgctcta tccgcacgtt cgcagcccgc tctatttcga gattcatcgc gtcggcgagt
     4801 tcacggcgcg ctcgctcgat atagatgtcg tgctcgatct gatgattcac gatctggatg
     4861 tcgtgcagtg gctcgtcggg agcgacgtgg aagtgacgga cgtgcgcgcc gtagggattc
     4921 ccgtcctcac cgagcgcgtg gatgcgtcca acgcgcgcat cgagtttgct tccggcgcgg
     4981 tcgccaacat caccgcctcg cgcgtcggca tggagaagat tcgcaagatg cgtttctttc
     5041 aaccacacga ctacatcgcg gttgactacg ccgcccgcca cacgtcgatc agcagcctcg
     5101 cgccgcccga tccttcgagc gcgcgccccg gcgtacagtt gcgccgcttg gagatcgccg
     5161 acgttgagcc gctgcgcgcc gagatcgaag cgtttctcga agcggcctcc acaggccagc
     5221 cgccgcccat cagcggcgcg gacgggcggc gcgcgctctc gctcgccctg cgcgtcatcg
     5281 aacgtatcaa cgaacacacg accaacgccg gactccatgc actggccgcc gccacgcgtt
     5341 caaccttcaa aacacccggc gacacttaac acggcgaagc acacgttgat ctccaaccgc
     5401 actcacgaag tatcaatcga cgcgcttctc gccggacgca tcgcagcggg cgacttccct
     5461 tccgccgtct atctcgtagc cgagcgcgag cgcgtcgcgt tcgccaatgc cctgggcgac
     5521 gccgtgcgcg aacccgtgcg acacgcggcg accgtcgaga cgatctacga tctcgcgtcg
     5581 ctgacgaagc cgctcgtcac gggtctgctc tgcgcgcgtc gcgtcgagcg cggcgagttg
     5641 gaaatggatc gcgccgtcgc gtcgtacctg ccggagttcg cgccggaggg cgacaaggct
     5701 tcgatcactt tgcgccattt gttgacgcac acttccggtt tgcccgcgtg gcaaccgctc
     5761 cgcttcacgt ctcataaccg ccgcgaacgc gcgctggcaa caatcgccgc cactgccctc
     5821 tcgtacccga ccggctcgcg cgtcgtctat agcgatctcg gtttcatcac actcggcctg
     5881 ctgctcgaaa aaatctcagg cgcgccgctc gacgagttgg cgcagagtga gattttccaa
     5941 ccgctccaac tccaaagaac atttttcaga cccgaacagg cttatctgac cggagtggcc
     6001 gcgtgcgaac tgtcgggcaa cgcgtatgag cgcggcatgt gcgaagcgga agggcaggcg
     6061 gagagcgcgg gcgcggcggg cgcatggcgc gaagagttga tctggggcga ggtgcacgac
     6121 gggaacgcct atttcctcgg aggcgcggcg ggacacgcgg gacttttctc gaacgcgcac
     6181 gagacgtttc gccttgcgga acagttcatc gcccgccgca cgcaactgct cgcgccggag
     6241 acgtgcgagt tgtttcgcac gaacctcacg ccggatttga acgaagcgcg ttccttcgcc
     6301 tggcagttgg ccgcaacgcc ggactctacg gcgggcgacg cgctcgcgcc cgactgtttc
     6361 ggtcatctcg gattcaccgg gacgagttgt tggattgacg ctctgcgcga gcgcgtcttt
     6421 atcctgctca ccaaccggac gcacacgcgc acgctcccct tcgtcaacat caacggcgtg
     6481 cgccgacgct ttcacacgct cgccgtccat gcgctcggag acgcgggcag tttcgtcaac
     6541 catccgcaat gaaaccactc gacgagacat cccggcacga atccgcgctt tcgcctgagc
     6601 atgttaaggc ggcggcgggg cgcgtctcgt tcggcgaagc gtttcgtttc tgggtgaagc
     6661 tgggattcat ttcgttcggt ggtccggcgg gacagatcgc gatcatgcac cgcgaactgg
     6721 tcgagcgcag gcgttggctg agcgaagagc gtttcctgca cgcgctcaac tactgcatgc
     6781 tcctgcccgg ccccgaagcg caacaactcg ccatctacat cggctggctc atgcaccgca
     6841 cgtggggcgg catcgtcgcg ggcgcgttct tcgtcatccc ttcgatcttc atcctgctcg
     6901 cgctctctta cgtctacgcg aaatatggac aactcacttc catcgccggg gtggtcaacg
     6961 gtttcaagcc ggtcgtggtg gcaatcgtcg tcgaggcgtt gttgaagatc ggcgggcgcg
     7021 cgctcaaacg tcgcatccat tttctcatcg ccgtcgccgc cttcgccgcc atcttttttc
     7081 tgcacattcc cttccccgtc atcgtgctcg cggcgggtct gctcggactc gcgggcgcac
     7141 gcttccggcc tgacgttttt gcgccgcctg cgccgaaggt gtcgaaatcc gcccaaccct
     7201 caaagagtaa agagacgaga gaggagacgg ctcgacgcga agtttcacac gacgctcaag
     7261 ctcgcgcagc gttcgtgatc gcagacgacg cgccgccgcc cgcgcacacg ctgccttcga
     7321 gcgggcgcac gctcagaatc ctcttagcgg gaatcgtgct ctgggtgctg cctttcgccg
     7381 cgatcatctg gtggcgcggt tgggggagcc tgcacgcgga ggaatatcgc ttcttcacac
     7441 aggccgcgct cgtcaccttc ggcggagctt acgccgtgct cgcttatgtc acgcaggccg
     7501 ccgtcggttc cttcaactgg ctcacgcacg cgcaggcggt tgacggcctc gcgctggccg
     7561 agacgacgcc cggaccgctc atcatggtgc tgcaattcgt cggcttcatg gcgggttgga
     7621 accacccgcc gcaaggttta gaccagacga cgagcgccat cgtgggcgca ctcgtcacca
     7681 cctacgccac tttcctgccc tgcttcatct tcatcttcct cggcgcgcct tacatcgaga
     7741 tactgcgcgg caacaagaat ttgacgagcg cgctctcagg catcacggcg gctgtcgtcg
     7801 gcgtgatact caacctcgcg ctcgtcttcg gcgcggcggt catctggccg cacggtcttt
     7861 caggcggcgc gaacttgttc gcagcgatca tgagcgtggc ggctttcgtc gcgctgtacc
     7921 ggctgaaact cgatgtcctg tgggtcgtac tcgcgggcgg cgcgctggga ttcctgcatg
     7981 gatggttttt cgctcgatga cgaatgcccg gctctatccg cactctatcc gctaacagaa
     8041 cgggaggcga attttctgct ctgcggtcgc ctgcgttccg ccggagttgt agtagtatga
     8101 aggcgcggct gtctgagatg taaagccgct gccgggaagt acgattgatg ccgatcctgc
     8161 ctttcaagtt tacgaagctc atcgcgttgt ccgtcgcgct cgcgctgctc gcacagtctt
     8221 tcgtgtcggc gcaggacgct gcgacgcaga cgcgcccgcg ccgtgtccag acggagacgc
     8281 cgcccgtttc aaccatccaa cctccgcgcg tagatgtcgc cgtcacacga cccggcgcgg
     8341 agccgaacat tcgcatcggc ttatccacca acgcgcgctc cgtcacgatc tcgacgagtg
     8401 ggcaagcctt gacggtgctc cggacgcagc ccggcgaaat gcacgcgccc gccgcgctcg
     8461 ccgtctcgcg cgtgcgtatc gaaccgcgca tgctcatgcc gcttcctgcg ccgcgcgcgg
     8521 atgagaacgg cctgtttcgc gtagaggtcg cggcggtgac gagcgaggcg caggccgctt
     8581 caatcgcgcg cgacgtgaaa gaattgacgg gcgaagcgcc ggaagtcgtg cgcgatgcgg
     8641 cgacgacgtg gcgcgtgcgc gtcggcctcc ccacgccgcg cacggaggcg gaagaactgc
     8701 gcacgcggct cgaagaagcc ggtatcgcgt cagtctccct cgtcggcgtg caacgtcccg
     8761 caggcacgac gccgcagcca cagacggagg ccgcacgcgc acgcgaaaac tcaaacgctc
     8821 cgacctctgc gaagcgcacg caaaacagtt cgacggcgca caggagcgag agcaccggcg
     8881 tgcggctcgc cacgcgcgcg acgctgccga cgcgcggcct cgtcgtctct gcgggcggca
     8941 cgtcgccgct gctcgactcg cgcgcgcccg ttacgttcgc gcccaccgaa gcgagtgcgc
     9001 cgcttcgctt caacgaaaaa ccttatcgcg ggcaactcga agttttcacc aacctgaacg
     9061 gctcgctcac ggtcgtcaac gtgctcggcc tcgaagagta cgtgcgcggc gtcgtgccga
     9121 acgaactttc gccgggtggc tatggcgcgc tcgaagcgtt gaaggcgcag gccgtcgccg
     9181 cgcgcactta tgccgtcagc aatcgcggcc agttcaacgc cgctggtttc gatctgttgc
     9241 cgacgattcg ctcgcaggtc tacggcggac tctccacgga acatccgctc tccacgcgcg
     9301 ccgtggaaga gacgcgcggc ctcatcgcaa cctatcgcgg cgaaccgatc aacgcgctct
     9361 acacctcgac ttgcggcggg cgcaccgaac acgccgagaa cattttcggc ggcgagtttg
     9421 tcccctacct gcgcgcgcgt gaatgtaccg cgcacgccgc acacgcatcc ttagtttcga
     9481 ccaacattca aaccacgcgc gaaccgcaca acctgcgcga cgccctgcac gctgcgagtg
     9541 cacgcgaagg cgcgctgctc gccgtgcatg gctttcgtct gccgtcgcgc atcaacgacg
     9601 agtggctggc cgccgccgtc agcgcagaag aggttcgcgc gctgctcgaa gtggccgcac
     9661 ggctcgcacg acgccccgcg ccgcttgcct ccatcaacaa cgatgcgacg cgcccgcccg
     9721 gattcagcac cgcgctcgcg ctcgcgctcg acggggagag tcgcgccgat gtcctgctct
     9781 cggcagacga cgtgaactac ctgctcgcct tccgcgacgc cgaagagatt cccgcgcaga
     9841 accgcgccga cgtggcgttg ctcctgcgcg acgggcatct cacgctccac acggacgcca
     9901 ccctgcgccc gcgccagccg atgtcgcgcg cacgcgccat gcacacggtc atccacctgc
     9961 tcgaagcgcg cggactcttg cagttgcaga aagcgacggc gcgcccgccc gtcggcggcg
    10021 cgctcgtggt gcgtgcgggc aagtcgcctg agcgcgccat cgccgtcgcg cccgatgctt
    10081 acctgttccg cacctttggc gagcaacttt ttcaaatgcg caatgtgaac gtcgtcggcg
    10141 gcgagccggt ggcgtatcac acggatgcgc gtggcgcggt cgattatctg gagttgcaga
    10201 ccgcgccgaa gggcgcgtcc gccgaacgcg tctcgcctta cacgacctgg acgaccactc
    10261 tcagcgcggg cgaagtcgcg cataggctgg cgcgcagcgc gggtcgcatc ggctcactgt
    10321 tggatttgcg cgtcgcctcg cgcggggctt cgcgtcgcgc gctcgatctc gaagtcacag
    10381 gcacgacggg gacggcgcac gtgcgcggcg gtcggattcg ctccgcgctc ggcttgcgcg
    10441 aacaactctt cgtgattgat cgcaagtatg acgagagcgg gcgggtggca agtttcgtct
    10501 tcaccgggcg cggctggggg cacggcgtcg ggatgtgtca ggtcggcgca tacggaatgg
    10561 cgcgcgccgg actccgctat gatcagattc tcaagaatta ctacaccgac attgccctga
    10621 cgaagatgta ttgatccgag attgcacgat tgagagtcgt atgaaagaga cagaaacagg
    10681 ctcagccgtc acgcttttcg tcgtcccgaa ttgccctctc tgcacgcacg cgcgtgcgtg
    10741 gctggcgcaa cacgagataa ctttcgtcga acgcgacgta aaacgagact tcggcgcgct
    10801 gcgcgacatg tatcgtctga cgggacaaaa tctcgtgccc gtctttgcac gcggcgaaca
    10861 cgccctcgtg cgtccgaccg acaaacaact gacggaattt ctcgtaaatc gtcaaagaac
    10921 gtgaatcgtg aatcgtaaat cgtaaataga gaaaacctgc tttcgtctat ttacgattca
    10981 cgattcacga ttcacggcac ttgcttatgc gtttctcgcg tgcacaactc gtcggcgcgt
    11041 tcgcgctgct cgcagtcatc tgggctgtgc tgatctttcg tctcttattt tccgcctcgt
    11101 gagttccctc tccagacatc aaccgatcat cgtcgtcgcg gggccgacgg cttccgggaa
    11161 gagcgaactg ggcatcgccc tggctctccg cttcgaaggc gagatcatca attgcgactc
    11221 cgtacaaatc tatcagggca tagagatcgc cacggccaaa gtgccgcccg cagagcagcg
    11281 cggcgtgccg catcatctgc tcgacttcgt tccgccccac gtcaacttca ctgcgggcgc
    11341 gtgggctgag acggcgaccc ggacgctctc ggagatcgaa gcgcgcgggc gtctcgccat
    11401 cttcgtgggc ggcacgggct tctacctgcg cgcgctcaga gaaccgttct ttccgagtcc
    11461 gccgacggac gaagcgttgc gggcgcgcct gacgcagctc agggagcgac gcggcgcaga
    11521 gcacctgcat cgtattttgc gccgtctcga cccggcttcc gcttcgcgtc tccacccgcg
    11581 cgactggtcg cgtgtacagc gtgcgctcga agtgcgcgtg cagacgggcg cgccgctctc
    11641 tctgcaacaa cccgcgcgcc ctgcgccgcc gcccttcgcc gcgcgcctgc acatctttgc
    11701 cctctcgcct ccgcgcgacg cgctctacgc gcgcatcaac gaacgcgccg cacgccattt
    11761 tcaagcgggc ttgattgaag aagtgagccg actgttaaag gcaggcgtgc ccgccgcttc
    11821 caacgcgctc ggcgcgcacg gttatcgccg cgcggtcgaa tacctgaacg gcgcgcgcac
    11881 gcttgagagc gccatcgaac agaccaaact cgacgtgcgc cattacgcca aacgccagtt
    11941 gacatggttt cgccgcgaac ccggtgtcga atggatcgag ggcttcggcg acgaaccgga
    12001 aacgcaggag cgcgtggcgg cgcgcgtcgg cgagttgctg gcgattaacg ccgggtgagc
    12061 cttgaaattg acgttggcgc gagattaagg ggcggcagga gtgcggcaag agtcgccgca
    12121 tttttcgttt cgggcggcct tcgtcaacat tagcttgtca cgcgtcacag cgtgttgtaa
    12181 tctgacgaca ctgtttaatc gcgagttgat ccgcatggag tgttgccgtg gactctaagc
    12241 cttctcccca aaatattcag gacgccttcc tgaacacggt acgccgtgag aaagacgccg
    12301 tcacgatcta tctgatgaac ggcgcgaaac tcatgggtcg catcaagagt ttcgacaaat
    12361 tctcggtgct gctggaagcg ggcgcgcagg aacagttgat cttcaaacat gcgatctcaa
    12421 ccatctcgca cgcgcgccgc cacgcgagcg gcgaactgcg cggcgcacac tctttcgaac
    12481 cggcgggcga gcacaatagc cacgacgcct ccgaacgcgc cgacgattaa cctctcccgc
    12541 agccagacca tctgacggag tcccaacttt tttgaacggc gcatttatca cctttgaagg
    12601 catcgacggg tcggggaaat ctacccaggt gcggatgctg tcgagcgtgc tccgcctgcg
    12661 cggcatggac gtggtgcaga cgcgcgaacc cggcggcacg ccgctcggct cgcgcctgcg
    12721 cgaagccctg ctcgacgctc aggaacaggt tgacccgctc gcagaacttt tgctctacgc
    12781 cgccgaccgc gcgcagcacg tccgcgcgct cctgctcccc gcgctcgccg ccggtcgcat
    12841 cgtgctctcg gatcgttatg cagatgccac cgtggcctat caacagttat taaatgcaaa
    12901 aaacatactt tatactgtaa gtgtatgtat gggtgggtgt tttgcatttt gttttcgagg
    12961 atagatactt tatctatcta aaatgaaaca cagatatact tttccaagcg agcacgtaaa
    13021 atgagtttag gactttgcgg tccacataga actgggaaaa caacacttgc tcaggcactt
    13081 gcctcgcgta ctgggaggcc gtttgttccg acaaatgtca gcgcggtctt tgcagaatgt
    13141 ggaatagacc cagcagcacc aatagatttc cagacacgtc ttcgaattca aaatcaaata
    13201 ttggattctt gtgaaaaggt ttggcagaca gatagagcac ccttcataac tgatcgaacc
    13261 cctatcgatt tgatggccta caccctaggt gacatacagg gaacaactaa tgttaatttc
    13321 actgaattag agagctacat gcataactgt ttcgaggtga ctaataaaaa tttcgatgcg
    13381 ctcatcatac ttcagccggg cataccatta attaaagagg ggggcaaggc tgccctgaat
    13441 agtgcttata tagagcatct gaatagcctc attattggct tatgcaacga cgaacgcctc
    13501 aattgcggag tacttaaaat caagcgggaa atgacagacc taggtgaacg tgtccagaaa
    13561 attcttgggg cggggcttga tgtacgtgtg cttccgtgaa ctgtatagac tgatgttata
    13621 gactttcaat catctccaca tccggacttc tcccgtcgaa caggatagcg aactgattca
    13681 tcgctgcgct ccagttaggg atcggcattg tccacggctt actgatgttg cgcaaggcca
    13741 gatacatcag cttgaagacc gcctcgtcat tcgggaacaa ggagcgattc ttcgacactt
    13801 tgcgtagcgt catattcagc gactcgatgg tgttcgtcgt atagacagca cgtctgatct
    13861 ccgaaggata cccgaacatc ggcaccactc gcgcccagtt cgaccgccag gactttgcga
    13921 tcaccgggta gttggccgcc cacgtctcct cgaactcggt gagttgacgc tcggcctctt
    13981 cgagcgtggc tgcctgataa atcgtcttca ggtcatcggc tacagcctta cggtctttat
    14041 gtaacacgta acttaaagag tgcctgacca gatgcaccat gcagagttgc acctgcgtct
    14101 gcggatacag ggcttcgatg gcttcgggga agcccttcaa gccgcccaca caagcgatga
    14161 aaatatcgga gacgccgcgg ttcttcaact ccgtcacgac ctgcatccag aatttcgcgc
    14221 cttcattgtc agcggcccac atgcctaaaa cttccttcaa gccttgtaag ttcacgccga
    14281 aggccagata aatggattta ttcgtgactc ggccatgact cttgaccttc acctgcaagg
    14341 cgtcgagata gaggatggga taaacggagt cgagcggacg cgcctgccag gtgcggacct
    14401 catcgaggac ggcatccgtt accgtcgaga ttaaactcgg cgagatctcc accccgtaaa
    14461 tctcttcgag gtgcccctgt atctcgcgct gcgtcatgcc gcgcgagtaa agtgagatga
    14521 tcttctcgtc taacccgtcg aagcgcgtct gccccttctt gacgagttgc ggcttgaagt
    14581 ctgaggtatg gtcacgcggc acgtcaatct gcacttgacc gcgcttgccg cgcatggtct
    14641 taggcgtcgt gccgttgcgc gagttgccag agttatcgcc agcggaatca tgcttctcat
    14701 agccgaggtg gtgagtcaac tcgccgtcga gagcgcgctc aacaagggct ttagtgagtt
    14761 gctgaaggag gccgtcttga ccgagcaagt cttcgggcat ctggtagtct ttgagcagtt
    14821 cgtcgagcag ttcgggtcga atcggtggca taatcacatc tcctttcgag actgtatggt
    14881 tgattattgc cacttacaca gattttagag aacctcctta taaaccacac actcttttct
    14941 aggtgagtga tggaaaagtt cttggaataa tggagtgtct atgtctgctc tatcttttgc
    15001 aattatgctt ttaactttga gaaggaaagg cttaacttcc atatgtgcgc cactagaagt
    15061 tatctatata atttgtatct cagctaattt aatctttatc tttattaaca atttctgggt
    15121 cgagaatggt taattcatgt gcgaatcttt tctttagcac cagttgaaat tctgagcatc
    15181 tttcaatatc ttttctatca aataggattc tacttcttga gtcagctact tcatcaattc
    15241 catggatttc caagaattca taggcatcct ttagcgctga atcaactgcg cctattcgta
    15301 acttaactgt cgatgcggga ctaccagggg cgattcccca aaagtagcct gcatctctga
    15361 aatatttagg ctgctcagga ttggtgagcc aagatctaaa aacttgatta tccataatac
    15421 tttttattgt attgtgcaaa gctctcgtaa ctttgacctg aacagtttca tcatcaggtt
    15481 ttaatcgtgc tgcaaatgca agacctttgg ttgttagcct atagactcgg ctattctgtt
    15541 ttgctaaaaa cccatcatta accaagcctt tagcccctag agatttaccg cggctattta
    15601 tttccttact cagtctatct acatcaggat agaccttttc atagccacgc agcccccaag
    15661 cctcatgatc ctgcttccac gcttctacca atagatcctc taatgtaaaa ctcttgttaa
    15721 tgtctcctcc tgtacattta agcgcagcta agagagcctt ttcctttagc ccgaggcgtg
    15781 actcagtcat tccagtcctc caccgctctc aacgtagacc ttcaactcct gccacatcgc
    15841 ttgcttgagg tttatctgat ttcgttctag agattgttta tgtatcaacc aaaggtctgg
    15901 cctttcatct tcacccctac tagtgaactc ttgctgcata tgaacctgag ctaccatctc
    15961 gacaagttgc tgtgcatgaa acat
//
LOCUS       DASWNS010000105         8072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_315193, whole genome shotgun sequence.
ACCESSION   DASWNS010000105 DASWNS010000000
VERSION     DASWNS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315193"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4763)
                     /locus_tag="VGB76_03565"
     CDS             complement(<1..4763)
                     /locus_tag="VGB76_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MupA/Atu3671 family FMN-dependent
                     luciferase-like monooxygenase"
                     /protein_id="HEY0098013.1"
                     /translation="MKREFSSLVELLRHRALHQPDRLAYTFLAGGDVAETHFTYGELD
                     RRARAIASQLQAQGREGQRVLLLYPAGLEYIAAFFGCLYAGAVAVPTYPPRLNRNLLR
                     LQSIVTDAGATMALSTESLLSRVEPLLEQSPELQRLPWLSTDNLAANLADQWLEPALN
                     GDTLAFLQYTSGSTAAPKGVMVSHGNLLHNEGLIQRVFQQTEEAVIVGWLPLYHDMGL
                     IGNVIQPLYLGARCILISPVAFLQQPFRWLQLISEYKATTSGGPNFAYDLCVRKVTPE
                     ERATLDLSSWSVAFNGSEPIRRETLERFATTFAPSGFRREAFHPCYGLAEATLLVSGR
                     DGANLPFVNHAGQTLSEDGAGGEATANTTRSRNALVACGGALPGQKVLVVDPESRVER
                     LPGEVGEIWVSGRSVAQGYWNRPEETEHTFRARLADTGEGAFLRTGDLGFLQNGELFI
                     AGRLKDLIIIRGRNHYPQDIERTVELCHPALRPGCGAAFSLDETGVERLVIVQEQDQR
                     QQTDAGRIIDAIRQRVAQEHEVQASAVLLLKPGSILKTTSGKIQRRACREAFLKGELK
                     ITAEWREASADSQEASLSNLPLSFDSAESVAAWLQSEVAARVGVGAEKIDVKRPFIEY
                     GLDSLMATELMHSIESNLGVCISITDFLQGSSIAQIAEQATARTTDATREENAAALSA
                     EPEAATEFPLSHGQQAQWFLYQLAPESTAYNISAAVRIKGELNPDNLRRAFQMLVARH
                     PSLRTSFSAPLGEPLQKIQQSVALSFEIEEAANRDDAALQERLVEEAHSPFKLEEGGL
                     LRVRLFTRPAGEHVLLLSVHHIIADFWSLAVLIQELGALYEAEKSGVRAELPTLRLQY
                     GDYVRWQGRMLSGSAGEASWRYWERQLAGELPVLNLPADRPRPPMQTFRGASQSFKLS
                     EELTRKLKTLGVARGATLYMTLLAAFEVLLYRYSGQEEIIVGSPVAGRSRAELAGLVG
                     YFINPLALRADLSANPTFDSFLAQVRQTVLDAFAHQDYPFPLLVEKLHPHRDASRSPI
                     FQAVFILQRAHLLDDEGLASFALGETGAQMKLGELALESMALEQRVAQFDLTLMMAEV
                     DGGLGASLQYNSDLFDPATIQRMAQHFETLLESIAASPAQTVSTLPLLPQAERHQLLR
                     EWNETETNYPDEQCIQRLFEAQVERTPGAVALTFENEQVTYEELNRRANRLARFLKEL
                     GIEPDALVGILMERSIEMMVALLGTLKAGGAYVPLDPSYPAQRLAFMLENAEVKVLLT
                     QQRLAAGTVGVAARVVCVDTDWPEIATHEAENLEASASADNLAYVIYTSGSTGEPKGV
                     MVAHRNVVNFFTAMDQRIESEGVGKTWLAVTSISFDISVLELFWTLARGFNVILQREP
                     ERAALAAPSTVRRTNREIAFSLFYFASDDDQTSADKYRLLLEGAKFADRNGFAAVWTP
                     ERHFHAFGGLYPNPSVTSAAIATITEQIQIRAGSVVIPLHSPIRVAEEWSVVDNLSNG
                     RVGVSFASGWHSDDFVFAPHEYAQRKEAMLRGIEIVRKLWRGEAVTYQGGAGNDVEVK
                     ISPRPIQRELPFWITAAGSPETFQTAGESGANLLT"
     gene            complement(5110..7485)
                     /locus_tag="VGB76_03570"
     CDS             complement(5110..7485)
                     /locus_tag="VGB76_03570"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEY0098014.1"
                     /translation="MVRAQAHISTLTQPRPPVSSQVPLVVGIATLLFIVLLIFFSIYL
                     LKPPAIVPASAPVTEFSSGRAMEKLAVISKQPHPIGSDGHAVVRDYLLADLNAIGLKP
                     ETQKTTVADYRWGNSFRAATVENILARLEGSNDGKAILLVAHYDSVPTSYGASDDGSG
                     VATLLETAHALKAGAPLKNDVIFLFTDAEEVGMLGARAFVNEHPWAKDVGLVLNFEAR
                     GTGGPSVMFETSDKNGSIINEFAKAAPHPVANSLSYDIYKLLPNDTDFTVFRNAGWQG
                     LNFAYIDGLSHYHTSLDNFQKIDEGSLQHSGSYALSLTRHFGNLSLDGRGERNSVYFD
                     ILGRTLVSYSPTWAVALTALIVLMFAGVVLYGFKQGRLSVLGIILGFFVLLLNIIIAA
                     ALVMCVGWITRALQRGVSAIPQGDTSHSSLYLLGVAALVLASTSIIYIWFQKKTSIGN
                     LTLGGLLFLLILLILSHLYLPGGSYLLAWPLLFSLVALGYLFAAKDENHVSLKKQVVL
                     TLCAVPSLVLLSPMIYQLFVALGLGAAAMLGALMVLSLGMIMPQRNLAAAPKRWLLPG
                     GAAVASLLLIVAAVAGAGVNKDHPRQDSVFYALNADIEKAVWVSSDERLDEWSSQFFT
                     AGAQKGALTEYLPWGGEGYLKGAAPVASLSAPEVSVLEDRADNGGRLLRLRVNSPRQA
                     AIMSIFVDATVQQAVVNGKPVREGAEQPKRNPGKRWSLQYCAPPAEGIELTLSLKSME
                     PVSVVVVDESRGLPELPGSVYQPRPAHTISSPVLPSDLTFVRKTFTLKMQT"
BASE COUNT         1780 a         2359 c         2199 g         1734 t
ORIGIN      
        1 tgagtcagaa gattagctcc tgattcgccc gccgtttgaa atgtctcagg gctaccggcg
       61 gcagtgatcc agaaggggag ttcgcgttgg atcgggcgcg gtgaaatctt cacctccacg
      121 tcgttgcccg caccgccctg atatgtgacc gcctctcctc tccatagctt gcgcacaatt
      181 tcgatgcccc tcagcatcgc ttccttgcgt tgtgcatact cgtgcggcgc aaacacgaaa
      241 tcgtcggaat gccagcccga ggcgaaagag acgcccaccc tgccgttcga taaattgtct
      301 acgaccgacc attcctccgc gaccctgatc ggactgtgca gcggtatcac cacactcccg
      361 gctctgatct ggatttgctc cgtgatggtg gcgatggccg cgctggtgac ggacggattc
      421 gggtagagtc cgccgaacgc gtggaagtgg cgttcgggag tccacaccgc agcgaagccg
      481 ttccggtctg caaacttcgc cccttcgagg agcagccggt atttatctgc gctcgtttga
      541 tcgtcgtcgc tggcgaaata gaagaggctg aaagcgatct ccctattcgt tcgcctcacg
      601 gtcgaggggg cggcgagggc ggctcgctcc ggttctcgct ggagaatgac gttgaagcct
      661 ctggcaagcg tccagaacag ttcaagcacg gagatgtcga aggagatact cgtgacggcc
      721 agccacgtct ttccgacgcc ttcgctttcg atgcgctggt ccatcgcggt aaagaagttg
      781 acgacgttgc gatgagcgac catcacgccc ttcggctcac ccgtcgagcc ggacgtgtag
      841 atcacgtagg cgaggttgtc cgcgctcgca ctcgcttcaa ggttctccgc ttcatgcgtg
      901 gcgatctccg gccagtccgt atctacacag acaacccgtg ccgcgacgcc cacggttccc
      961 gccgccagac gttgctgcgt cagcagcact ttcacttccg cattttcgag catgaaagcc
     1021 agacgctgcg ccggatagga gggatcgagc ggcacgtatg ctccaccggc cttgagcgtg
     1081 ccgaggagcg cgaccatcat ctcgattgaa cgctccatca ggataccgac cagcgcatcc
     1141 ggttcaatcc ccaactcctt caagaaccgc gccagcctgt tggctctccg gttcaactcc
     1201 tcgtaagtga cctgctcgtt ctcgaaggtc agcgcgacgg caccgggcgt gcgctcaacc
     1261 tgagcctcga acaaccgttg gatacattgt tcatcgggat agtttgtctc tgtctcgttc
     1321 cattcgcgca gcagttgatg tcgctcggcc tgtggtaata acggaagcgt ggaaacggtc
     1381 tgtgcgggag aagcggcgat gctttccagc aaggtttcaa aatgctgcgc catgcgctga
     1441 attgtcgccg ggtcgaagag gtctgagttg tactgcaacg atgccccaag cccgccgtca
     1501 acttccgcca tcatcaacgt caggtcgaac tgcgcgacac gttgttccag agccattgat
     1561 tcgagcgcga gttctcccag cttcatctgc gcgcccgttt cgcctaacgc aaacgaagcg
     1621 agtccttcat cgtcgagcag gtgcgctcgc tgcaaaatga agaccgcctg aaaaatcggt
     1681 gagcggctgg cgtcccggtg cgggtgcaat ttttctacca gcaggggaaa gggataatcc
     1741 tgatgtgcga aagcatcgag aacagtctgg cgaacttgag ccagaaaaga gtcgaaggtt
     1801 ggattggccg ataggtctgc acgcaaggcc agggggttga tgaagtagcc gaccagcccc
     1861 gccaactcag cccggctccg acccgcgaca ggcgacccga cgatgatctc ttcctgaccg
     1921 ctatagcgat agagaagcac ctcgaatgcc gccagcagcg tcatgtagag agttgctccg
     1981 cgtgccacgc cgagcgtctt gagtttccgc gtcaactcct cgctcaactt gaaggattga
     2041 gatgctccgc gaaaagtttg catgggcggg cgcggcctgt cggctggcag gttcaacaca
     2101 ggcaactcgc ccgctagttg ccgctcccag tatctccagg acgcctcccc tgcgctgccc
     2161 gacaacatgc gcccctgcca acgaacgtag tcgccatatt ggagccgcaa cgttggaagc
     2221 tccgcgcgaa cgcctgactt ctccgcttca tacagcgcgc ccaattcctg tatgagcacg
     2281 gccagcgacc agaaatcggc gatgatgtga tggacggaca gcagcaggac gtgttcgcca
     2341 gccggacgcg taaacagccg cacacgcaga agcccgccct cctccagttt gaacggactg
     2401 tgagcttctt cgacgagccg ctcctgcaaa gcggcatcgt cgcggttcgc ggcctcttcg
     2461 atctcgaagg agagagcgac gctctgttga atcttctgta gaggctctcc gagcggcgcg
     2521 ctaaaacttg tacgcagcga aggatgacgc gccacgagca tctgaaatgc gcgccggaga
     2581 ttgtcaggat tcaactcgcc cttgattcgc acggcggcag agatgttgta ggccgtactc
     2641 tccggcgcga gttggtagag aaaccactga gcctgctggc cgtgagagag gggaaactcc
     2701 gtggccgctt ccggctcggc ggaaagagcg gcggcgtttt cctcccgtgt ggcatccgtt
     2761 gttcgggctg tcgcctgttc ggcgatctgc gcgatgctgg aaccctgtag aaaatcggta
     2821 atggatatac agactccgag gttggactcg atgctgtgca tcaactcggt cgccatcaga
     2881 gaatcgaggc cgtactctat gaacgggcgt ttgacatcga tcttttcagc ccccaccccc
     2941 actctcgcgg ccacttccga ctgaagccac gctgcgaccg actccgcact gtcgaaagag
     3001 aggggcaggt ttgaaagtga ggcttcctgc gagtcggcag aggcttcccg ccactctgcc
     3061 gtgatcttga gttcaccctt gagaaacgct tcgcggcagg cgcgtctctg aatcttcccg
     3121 ctcgtggtct tcaggatact tcccggtttg aggagcagca cggctgacgc ctgcacctca
     3181 tgctcctgcg ccacccgctg gcggatggcg tcaataatcc gacccgcatc cgtctgttga
     3241 cgctgatcct gctcctgaac gatcactaga cgctcaacgc ccgtctcatc gagggagaag
     3301 gctgcgccac agccgggtcg taatgccggg tggcacagtt cgacggtgcg ctcgatgtct
     3361 tgagggtagt ggttgcgacc ccgaatgata atcaggtctt tcaggcgacc ggcgatgaat
     3421 agttccccgt tctgtaaaaa tcccaaatcg cccgtgcgta gaaaagcgcc ttcgccggtg
     3481 tctgcgagac gggcgcgaaa cgtgtgctcc gtctcttcag gccggttcca gtaaccctgc
     3541 gccacgcttc gaccgctcac ccagatttcg cccacctcac ccggcaaccg ctcgacacgc
     3601 gattccgggt ctaccaccag caccttttgc cccggcagtg ctccgccaca cgcgaccagc
     3661 gcattccggc tccgggtagt gttggcggtt gcttccccgc ccgccccatc ctcagagagc
     3721 gtctggcctg catgattcac aaagggcaga ttggctccat ctcttccgct caccagcagc
     3781 gtcgcttccg cgagtccata acacgggtga aaggcttcac gacgaaagcc gctgggggcg
     3841 aacgtcgttg caaaacgttc caacgtttcc ctgcggatcg gttcggagcc gttgaaagcg
     3901 acgctccaac tgctcaaatc cagagtcgca cgctcttcgg gtgtgacttt gcgcacgcac
     3961 aggtcataag cgaagtttgg gccgccgctc gtcgtggcct tgtattccga gatgagttga
     4021 agccagcgga agggctgttg cagaaaagct acgggtgaga tgaggatgca tcgcgcgccg
     4081 aggtacaaag gctggatcac gttcccgatc aatcccatgt cgtgatagag aggcagccaa
     4141 cccacaatca ccgcttcctc tgtttgttga aatactctct gtatcaatcc ctcgttatga
     4201 agaagattgc cgtggctcac catcacgcct ttgggcgcgg cggtagagcc ggaggtatat
     4261 tggaggaaag cgagcgtgtc gccgttcaaa gcaggttcaa gccattggtc tgcgaggttc
     4321 gcggcgagat tatcggttga cagccacggc aggcgttgta attccggaga ctgctcaagt
     4381 aaaggctcga ctctggacaa gagcgactcg gtggagagcg ccatcgtggc cccggcatcg
     4441 gtcacgatgc tttgaagccg cagcagattc cgattaaggc gcggcgggta ggtcggcacg
     4501 gcgaccgcgc ccgcatagag gcagccgaag aatgcggcga tgtattccag ccccgccggg
     4561 tagagcaata aaactctttg cccttccctg ccctgcgcct gaagttgaga cgcaatagcc
     4621 cgcgcccgcc ggtctaactc tccgtaggtg aagtgggttt cggccacgtc gccgcccgcg
     4681 agaaaggtat aggccagccg gtcgggttga tgcaaagctc tatgccttag cagttcaacc
     4741 agagatgaaa attctctctt catgtcggaa ccagattcag ggtatgcgct catcgtactt
     4801 gacatccctc acgctctcag ccgcacaggc gaacggctat ttattcaaac aactctcccc
     4861 gcacaaacga agtttgcgtc tgcggctccg gggtgaacca tatcgctcag ggcggcgcgc
     4921 gacagtcgcg cccgagtcga agtcgtcttg ctgcttaaat agcccgcgcc ccgaaaaaat
     4981 ttagtaattg gtttaaggag taacggagaa gtaaccgtgt tgcacttgat tgccgtgttg
     5041 ctgcccgccg cacatcgaac gctaacggcc tactttcggc aagcggaagg gtaaaacacc
     5101 ccgccgcttt tatgtctgca ttttgagcgt gaaggttttt ctcacgaagg ttaagtcgct
     5161 cggtaacacc ggagatgaga tggtgtgcgc tggcctcggt tgataaacgg agccgggaag
     5221 ttcgggcagc cctctcgatt catctaccac gaccacgctg accggctcca tcgatttcag
     5281 agacaacgtt agctcgatgc cttctgctgg aggagcgcag tattgcaacg accaacgctt
     5341 gccggggttt ctcttaggct gctccgcgcc ctcccgaacc ggcttcccat tgaccactgc
     5401 ctgctgcact gtagcatcaa cgaatataga cataatcgct gcctgtcgcg gtgagttcac
     5461 ccgaaggcgc aacagtcgtc cgccgttatc cgccctgtct tcgagcaccg aaacctcagg
     5521 cgcgctcaaa gatgcgaccg gggctgcgcc ttttaaataa ccttcgccgc cccacggcaa
     5581 gtactccgtc aatgcgccct tttgcgcgcc cgcagtgaaa aactgggaac tccactcgtc
     5641 cagcctctca tcactactca cccagaccgc tttttcgatg tcggcattaa gtgcatagaa
     5701 gacactatcc tgccggggat gatctttatt gacacccgct ccggccacag ccgcgacgat
     5761 caacaacagg ctcgccacgg cagccccccc cggcaacagc caacgcttag gggcggcagc
     5821 cagattgcgc tgcggcatga tcatccctag cgacaacacc atcagtgcgc ccagcatagc
     5881 cgccgcgccc aagcccagcg cgacgaagag ttgatatatc atcggcgaca gcaatacgag
     5941 cgaaggcacg gcgcacagtg taaggacaac ctgttttttc agcgagacat ggttttcgtc
     6001 tttagctgcg aagaggtatc ccagcgcgac gaggctgaat aacaaaggcc atgccagcaa
     6061 gtagctccca ccgggaagat atagatgcga aagtatcagc aatatcaaca ggaacaacaa
     6121 tccgcccagc gttagatttc cgatgctggt ctttttctga aaccatatat agatgatcga
     6181 ggtcgaggcg agaaccagcg ccgccacgcc caacaggtat agactgctgt gagacgtatc
     6241 accttgagga atggcgctga ccccccgctg caacgcgcgc gtgatccacc caacgcacat
     6301 cacaagcgcc gcagcgatta tgatattgag aagcaatacg aaaaaaccaa gaatgattcc
     6361 taaaacactc agccgccctt gcttgaagcc atataggaca actccggcaa acatcaacac
     6421 gatcaaggcg gttaaggcta cagcccaagt aggcgagtaa ctaaccagcg ttcggcctag
     6481 aatatcgaag taaaccgagt ttcgttcccc tctaccatct aagctcaggt tgccgaagtg
     6541 acgtgtcaaa gacagggcat acgagccact atgttgaagg ctgccttcat caatcttttg
     6601 aaagttatcg aggctcgtgt ggtaatgact gaggccatcg atgtaggcga aattgagtcc
     6661 ctgccatccc gcgtttctga aaacagtgaa atccgtatcg ttcgggagca gcttgtagat
     6721 gtcatacgac agcgaattag ccaccgggtg cggagcagct ttagcaaact cgttgatgat
     6781 cgacccgttc ttatcacttg tctcaaacat tactgatgga ccgcctgtgc cacgggcttc
     6841 gaaattgagc accagcccga cgtctttcgc ccaggggtgt tcattcacga aagctctcgc
     6901 tcccagcatg ccgacctctt cggcgtcggt aaagagaaag atcacatcgt tcttcaaggg
     6961 cgcgcctgcc ttcaacgcgt gtgcggtttc aagcagcgtc gccacgccgg agccgtcatc
     7021 gctcgcccca tagcttgtcg gaacggaatc ataatgtgct acgagtaaga tggctttacc
     7081 gtcgttactg ccttcaagcc ttgccaggat attttccacc gtagcggcac ggaaactgtt
     7141 gccccatctg taatccgcga cagtggtttt ttgtgtttcc ggttttaatc cgatggcatt
     7201 taagtctgcg agaagataat cacggacaac tgcgtgtccg tctgagccaa tcggatgcgg
     7261 ttgcttgctt attaccgcaa gtttttccat tgcccgcccg gaggaaaatt ccgtgacagg
     7321 cgcgctcgca ggcacgatgg cggggggctt taatagataa attgaaaaaa atattaacag
     7381 gacgataaac aaaagcgtcg caatcccaac cactagagga acttgtgacg aaaccggggg
     7441 tcgcggttgc gttaaagttg agatgtgagc ctgtgcgcgc accatattct ctgacctcca
     7501 tgaatctttc actggtaaag aggatcacta ttaaattttg aggagcttat tgtctgtcag
     7561 acttgcactg cattttgaat aaggagggat ccgaaaaact gtaattatat cgccgagaat
     7621 ttgaataacc gtgcaaaata gtctgttctt aaggcaaagc taccgcatat tgagccaagc
     7681 actcaatttc gaactatgaa aatcaaggag agttacagtg ctggatcagg cttaatacga
     7741 aagaatttgc ctgaccctac tccttgatct ttaacaccta cccgtttatc gctaagtggt
     7801 gcgtgcagat gccggaaact ctaagctgac atctgaactt cacgttcgtg gactaatcgc
     7861 tacagccagg tcaaaacttg ttggggcgaa tggttcacac ggtgcggctt tattctcttg
     7921 gtagacgccc gacattccgt acagacacat gtcgctggct aacttataaa gtgactttct
     7981 ttgaccgatt cttcttcgat aataagatgg atatcgtagg ttattgcttc gaacaatatg
     8041 cgcacacggt cagaattttt gattttcttg tc
//
LOCUS       DASWNS010000106         8102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_159552, whole genome shotgun sequence.
ACCESSION   DASWNS010000106 DASWNS010000000
VERSION     DASWNS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159552"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1933)
                     /locus_tag="VGB76_03575"
     CDS             complement(209..1933)
                     /locus_tag="VGB76_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098015.1"
                     /translation="MQHSPFTRVGWEVREHLARINPDGTLDDPFRSGANDSVRAIATR
                     SDGRTLIGGLFTQVNGQSRNRFARLNADGTLDIAYNPEPNGPVHAVGGLEDGRAFIGG
                     EFTEAGGRVRRRVARLVAGDSYPDGANMADPSFKLPQAGWGMAVQPDGRILASGVSGQ
                     SSLLTRFNVDGTIDQSFSCYVGGGEVHAIAVQPDSKIIIGGRFRYAGSQPRPYLARLN
                     GDGTLDSTFAQFTSSFSETTVNIIAVQADGRILVAGPFRKEAGHPRNYFARLNADGTI
                     DESFNPNPDKNPTSLALQPDGKILLGGGFSRIGGQERRGVTRLNADGTLDTSFNPNVG
                     ATAHAMAVQPDGRILIGGLFDYIGGVRRPSLARLKSDGTLDNFKPWGGSYYAPFSVTS
                     IALQTNGKIIISGMSGSASEVRYMRLNADGTVDTSHNSYRDGSNYFVLLQPDGRPLMG
                     ASGLKRLLNNEAAQQQLSVKLWADDTASIHWARSGAAPEVERVVFEVSTDGHTYNPVG
                     EATRGAGGWFLNGATLPLGRSIYVRARGTYMATGYVSPHTYSSTAIHDSVLYLHLYPN
                     QTDGPPTY"
     gene            complement(2245..5145)
                     /locus_tag="VGB76_03580"
     CDS             complement(2245..5145)
                     /locus_tag="VGB76_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0098016.1"
                     /translation="MVNGGKGRTAALILAALLFCSNFAFAQSSTGTLRGQVQDVLGGL
                     VVGVTVTATDAAGVARTATTSDEGQYVFAALPPARYTVRVESPGFDVYENTEVEVTAG
                     TTNALDIILNVAIEAEEVTVTSEAPISTDPESEAGAVVIRGAQLDALPDDPDSLADAL
                     QALAGPSAGPEGEGEIYIDGFSGGRLPPKESIREIRINRNPFSAEYERLGYGRIEVFT
                     KPGTDKFRGQTFFNFSDESLNARNPFAFTRAPFQSRRFGGNLSGPIIPGKVSFFFDFQ
                     RNETDENDVINAVILDPSLNPIAFNQSILTPARRTTFSPRIDWQLNPANTLVARYTYQ
                     RSRSENEGIGDFNLPERAFNSEGTQHTFQLTETAVINQQVINETRFQFERNRSIRQGG
                     LSAPTLRVQEAFTGGGAQVGLSTNDQNRFELQNFTSWVSGNHSLKAGARFRHLRLTDV
                     SQQNFAGTFTFTSLEQYRQTLLGLAGARPAQFSISAGDPEARITRSDFSPFIQDDWRV
                     RPNLTISAGLRYDWQTDIGSGLNFAPRLAFAWSPDGGQGRRQQTVIRGGFGIFYDTLR
                     ENLLLQAERFNGVNQQQFLVTSTSPGGEEILGLFPTIPSIETLRAFSVPQIVRQLAPD
                     LLTPYTLQTAFSVERQLPWRTTLSINFVNARTLHVLRSRNINAPLPGTLLVRPLPDQG
                     NIFQYESSGRFNQQQLIVNVNNRFSQRLSLSGNYVFNRARSDTDGANTFPANQYDLSG
                     EYGRSAQDIRHRFTLFGTITGLPWDIRLSPIVIANSGRPFNITLGRDVNGDTLFTERP
                     AFANDLSDPGVRVTPFGTFDVTPSAGQAMIPRNYATGPSFFVVNLRTSKSFGFGGVVA
                     PTAGAGGDATGGGGGGLGGGRRGGRGGGGGRRGGSGGAGGDISERYRYNLNLSVNVQN
                     LFNNTNAGQPVGNLSSPLFGQSIGSAGGFGRGGGGGQAAGNRRVELQLRFSF"
     gene            complement(5259..6530)
                     /locus_tag="VGB76_03585"
     CDS             complement(5259..6530)
                     /locus_tag="VGB76_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098017.1"
                     /translation="MKNQLHTIASAFLNISLVVVSIGHLSPVAAGAVSRAAQQTQHLV
                     GEVTALDQASGQITVKTDAGSSVTVAADERTTYRRLPPGQTSLDKAEGITRSDIKVGD
                     RVLVPNGAAGGQMAARQVIVMAREAIAARRQQEREDWRARGVNGRVVAIDPAKKELSI
                     EARSRQDVETITVVASGDVKFRRYAPGSLRPADAVPGAFTDIRVGDQLRALGNRTGSR
                     LAAEEIISGTVARLTGTVEAVDATRGEITIRDNSTGQPIIVAFNANTTLRRIPAEYAE
                     TLRERREQRREQRREGGEASAGGRQDGERRGGRRNQSRQEGRSQDGQRQEGQRQPDGQ
                     RAGGGGARQMLENLPPITLSELKKGDAVVVTGTAGTDAARVTAATLITGDAEIIQRMR
                     RFQRGSDPADGMSPGLPGGVIGGGTGDRDTP"
     gene            complement(7239..>8102)
                     /locus_tag="VGB76_03590"
     CDS             complement(7239..>8102)
                     /locus_tag="VGB76_03590"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEY0098018.1"
                     /translation="EHTVAEAASLLTSAGIDVQRERARGALDFSTSREAYLPSGHFEP
                     DERLTRLGEYVRQAIASGFSGLRSIGEMGWIDCEAPGCERAAEYEARLNDLIPHLPVT
                     GMCQYNRGQISPAILSDALRTHPVVVLNSKARRNLYYVPSAIFLGRASDIERVAWMID
                     ELNHAPEVTAGSPVLVVDDDQEIRQSMGRKLEALGYTVVKAEDAPGALEVARREQPYF
                     ILTNADLPWLGNLIYRLREEPGLRDAPVVSIYPNRPEEFHEDRIVVLDDYRQLEELWP
                     SKAASRLSINP"
BASE COUNT         1502 a         2419 c         2535 g         1646 t
ORIGIN      
        1 cgaaggagtg atgatgagca ggccagcgta gatcttcaca cacgacggga taacctacgc
       61 gccgtgtatt caatatgatg cgcccctccg gctttgaacg ccgggcatgt tatcgccttt
      121 cagccagacg aaaggggcga gagttagtct ctcgccccct cgccttcgtt ccgtacgcag
      181 gccgcttgcc cctgtccaac agcctgcctc agtaggtggg cgggccgtcc gtctggttgg
      241 ggtacaggtg gaggtacagg acggaatcgt gtatggcggt gctggagtag gtatgagggg
      301 agacataacc ggtcgccatg taagtgcctc gtgcgcggac gtagatggac ctgcccagcg
      361 gcagcgtggc accgttgaga aaccagccgc ctgcgccgcg cgtagcctcg ccgacgggat
      421 tgtacgtgtg gccgtcggtc gaaacttcga agacgacccg ctcgacctca ggtgccgcac
      481 cgcttcgcgc ccagtggata gaggcagtgt cgtcggccca cagtttgacg gagagttgtt
      541 gctgcgccgc ttcgttgttg agcagacgtt tcagcccgga cgcgcccatg agcggcctgc
      601 cgtccggctg caaaagcacg aagtagttgg agccatccct gtacgagttg tgggatgtgt
      661 caaccgtccc gtcggcgttc agacgcatgt agcgcacctc ggatgcggag ccactcatgc
      721 cactgatgat gatcttgccg tttgtctgca aagcgatgga agtgacactg aagggggcgt
      781 aatagctacc cccccacggc ttgaagttgt ccaacgtacc gtcgcttttg agccgcgcga
      841 gagacggacg gcgcaccccg ccaatgtagt cgaacaagcc gccgatcaga atgcgtccgt
      901 ccggttgcac cgccatggca tgagcagtcg ccccgacatt cgggttgaag gaggtgtcca
      961 gcgtgccgtc ggcgttgagg cgcgtcacgc ctcgacgctc ctgcccgcct atccgtgaaa
     1021 aaccgccgcc gagcagaatc ttgccatccg gttgtaacgc gagggacgtg gggtttttgt
     1081 ccgggttcgg gttaaaactc tcgtcgatgg ttccgtcggc gttgagacgg gcgaagtaat
     1141 tgcgcgggtg ccctgcctct ttcctgaatg gcccagccac cagaattctg ccatctgcct
     1201 gcaccgcgat gatgttcaca gtggtttcag agaatgagct ggtgaactga gcgaaggtgc
     1261 tgtcgagcgt accgtcgccg ttgagtcgcg ccaggtatgg acgcggctgg gaaccggcgt
     1321 acctgaacct gccgccgatg atgatcttgc tgtcaggctg caccgcgatg gcatggactt
     1381 caccgccccc cacgtagcag gagaaggact ggtcaattgt accgtccacg ttgaatcgcg
     1441 tgaggagaga agattgaccg gacacaccgc tcgccagtat gcggccatca ggctgcaccg
     1501 ccatccccca gccggcctgt ggcaacttga atgaagggtc agccatgttc gcgccgtcgg
     1561 ggtagctgtc cccggcgacg agccgcgcca cgcgcctgcg cacccgaccg cctgcttccg
     1621 tgaactctcc cccgatgaag gcgcgcccgt cctccagtcc accgacagca tggacggggc
     1681 cgttcggctc cggattgtag gcgatgtcca gcgtgccgtc ggcgttgaga cgtgcgaagc
     1741 ggttgcgtga ctgcccgttg acctgcgtga agagtccgcc gatgagcgtt ctgccgtcag
     1801 accgggtcgc aatagcccta acgctgtcgt tagcgccgct ccggaagggg tcgtcgagcg
     1861 ttccgtcggg attgatgcgc gcgaggtgtt cgcgaacctc ccagccgacc cgcgtaaagg
     1921 gggagtgttg caaaaacttt acgacgatac aagatattga ggcggcagca ctttttcagc
     1981 tcaactaatt gcgggcaaaa tattttttgc cacactacct cgtcaactgg gtcaatgccc
     2041 accatgcagc gttgctcgaa gctgagccgt ctgcgtctac gcccgccgtg gtcgaactcg
     2101 acgagttgtt caccttcgtt ggctcaaaaa aacgcaagcc tatgtcgtcc acagcggtag
     2161 accgggcaac gagctgcatg gctttgccca aaaagtcaaa aacttgtgga tatgtagagg
     2221 acgcccaaca gcgattctta tttctcagaa gctgaaacgt aattgcagtt cgacgcggcg
     2281 attccccgcc gcctgtccgc cgccgccacc tctgccgaat cctcccgcgc tgccgattga
     2341 ttgaccgaag agtggcgagc tgagattgcc gaccggttgg cctgcgttag tgttgttgaa
     2401 gaggttctga acattgaccg ataagttcag gttgtagcga taacgctcgg agatatctcc
     2461 gcccgcgccc ccgctgcctc cccgacgacc accaccgcca cctcgtccgc cgcgtcgtcc
     2521 gccgccaagt ccaccgccgc cgccacccgt tgcgtctccc cccgccccgg ctgtcggcgc
     2581 gaccaccccg ccgaagccga aggacttact tgtgcgtaag ttcacgacga agaaagaagg
     2641 ccccgtcgcg tagttgcgcg ggatcatagc ctgaccggcg gacggagtaa catcaaatgt
     2701 tccgaaaggc gtgaccctca caccggggtc agacagatcg ttggcgaagg cgggtcgctc
     2761 ggtaaataac gtgtctccgt tcacatcacg tccgagggtg atgttgaagg gtcggcctga
     2821 gttggcgatg acgatggggc ttagtctgat gtcccagggc agcccggtga tcgtcccgaa
     2881 gagagtgaag cggtgacgta tgtcctgcgc agacctgccg tactcgcccg acaaatcata
     2941 ctggttggcc gggaaggtat tcgccccgtc cgtgtcgctc cgcgcgcggt tgaagacgta
     3001 gtttccagac aggctgagcc gttggctgaa gcggttgttg acattgacga tgagttgttg
     3061 ctggttgaaa cgtccgctcg attcatactg gaagatgttg ccctggtccg gcagcgggcg
     3121 cacgaggagc gtgccgggga gcggggcgtt aatgttgcgc gagcgcagga cgtgcagggt
     3181 gcgcgcgttg acgaagttga tggagagcgt cgtgcgccag ggcagttgcc gctcgacgct
     3241 gaacgcggtc tgcaaggtgt agggcgtcag cagatcaggt gccagttgtc tgacaatctg
     3301 cgggacggag aatgcccgca gcgtctcgat ggacggaatc gtggggaaca ggccgaggat
     3361 ttcttccccg ccgggagatg tgctcgtcac gaggaattgt tgctggttca ctccgttgaa
     3421 ccgctcggcc tgcaagagca ggttctctcg cagggtgtcg taaaaaatgc cgaagccccc
     3481 gcgaatcacg gtctgttgtc gccgcccctg tccgccgtcc ggcgaccacg cgaaggcgag
     3541 gcgcggggcg aagttcaagc ccgagccgat gtctgtctgc cagtcatagc gcagcccggc
     3601 ggagatggtc aggtttggcc tgacccgcca gtcgtcctgt atgaaggggc tgaagtcgga
     3661 gcgcgtgatg cgcgcctccg ggtcgcctgc ggagatggag aattgcgctg gtcgcgcccc
     3721 cgccaacccg agcagtgtct gccggtactg ttcaagcgag gtgaaggtga aggtgccggc
     3781 aaagttctgc tgtgagacat cagtcaaacg gagatggcgg aatcgcgcgc ccgccttcag
     3841 agagtggttg ccggaaaccc acgaagtgaa gttctgcaac tcgaaccggt tttgatcgtt
     3901 cgtcgagagg ccgacttgcg cgcccccgcc cgtaaaggct tcctgcacgc gaagtgtcgg
     3961 cgcggagagt ccgccctggc ggatgctgcg gttgcgctcg aactggaagc gcgtctcatt
     4021 gatcacctgc tggttaatga cggcagtttc cgtcagttga aaagtgtgct gcgttccctc
     4081 ggagttaaaa gcgcgctccg gcaggttgaa gtctccgatg ccctcgttct cgctgcgcga
     4141 acgctgatag gtgtagcgcg cgacgagtgt gttggccgga ttcagttgcc agtcgattcg
     4201 agggctgaaa gtggtgcgac gcgcgggcgt cagaatcgac tggttaaaag cgatggggtt
     4261 gagcgacggg tcgaggatga cggcgttgat cacgtcgttc tcgtccgtct cgtttctttg
     4321 aaagtcgaag aagaaagata ccttgccggg aataatgggg ccgctcagat tgccgccgaa
     4381 gcgtcgcgac tggaagggcg cgcgtgtgaa cgcgaaaggg ttgcgcgcgt tcaggctctc
     4441 gtcgctgaaa ttgaagaacg tctggccgcg aaatttgtcc gtgcccggtt tggtgaagac
     4501 ttcgatccgc ccgtatccga gacgttcata ttccgccgag aaggggttcc ggttgatgcg
     4561 aatctcgcgg atggactctt tcggcgggag ccgcccgccg ctgaacccgt ctatgtaaat
     4621 ctctccttcc ccttcgggtc cggctgaagg accagccaac gcctgaaggg catccgccag
     4681 agagtctggg tcatcaggca gcgcatccag ttgcgctccc ctgatgacga ccgccccggc
     4741 ttcggactcc ggatcagtgc tgatgggtgc ctcggacgtg acggtcacct cctcggcttc
     4801 gatggcgacg ttcagaataa tgtcgagcgc gttggtagtt cctgccgtca cttcgacttc
     4861 cgtattttcg taaacgtcga agccgggcga ctccaccctc accgtatagc gggcgggcgg
     4921 aagtgcggcg aagacatact gaccctcatc gctggtggtc gccgtgcgcg cgacgcccgc
     4981 ggcatccgtc gccgtcaccg tcacgccgac aaccaagccc cccagtacgt cctgcacctg
     5041 cccgcgcaga gtccccgtgg aactctgtgc gaaagcaaag ttggaacaaa aaagcagcgc
     5101 tgccagtatc agggcggcag tccgcccttt ccctccgttg accattcgca ttatcccctt
     5161 ggtttcatag ttcggttagg tgcttctctt tttaaggggg gtgaaaagat gacgcagtaa
     5221 gaggggggtt aactaaatcg tatgtttccg cctccgggtc agggtgtgtc tctatcgccc
     5281 gtgccgccgc cgataactcc gcccggcaga ccgggactca taccatccgc cggatctgag
     5341 ccacgctgga agcgtcgcat ccgctggatg atctcggcat cacccgtgat gagagttgcc
     5401 gccgtcacgc gcgcggcgtc cgttccagcc gtgcccgtga ccacgacggc atcacccttt
     5461 ttcagctctg agagggtgat cggcggaagg ttctcaagca tttgccgcgc gccgccaccg
     5521 cccgcccgct gcccgtccgg ctgacgctgt ccttcctgac gctgtccgtc ctggctccgg
     5581 ccttcctgcc gggactggtt tcgccgaccg ccgcgccgct cgccatcttg tcgtcccccg
     5641 gcagaggctt cgcctccctc acgccgctgt tctctacgct gttctcttct ttccctgaga
     5701 gtctccgcat actctgccgg gatgcgccgg agagtcgtgt tcgcgttgaa ggccacgatg
     5761 atcggctgcc ccgtcgaatt gtctctgatc gtaatttcgc cgcgtgtcgc gtccacagcc
     5821 tcgaccgtgc cggtcagcct tgccacggtg ccggaaatta tctcctctgc cgcgaggcgc
     5881 gagccagttc ggttacccag agcgcgcaat tgatcgccta cacggatgtc tgtgaaagca
     5941 ccggggacgg cgtcggcggg gcgcagcgaa ccgggcgcat agcggcggaa tttcacgtcg
     6001 cccgacgcga cgaccgtgat cgtttctaca tcctgacgcg agcgcgcttc gatggagagt
     6061 tccttcttcg ccgggtcaat ggcgacgacg cgcccgttca caccacgcgc ccgccagtct
     6121 tcccgttcct gctggcggcg ggctgctatc gcctcacgcg ccatcacgat gacctgtcgc
     6181 gctgccatct ggccgcccgc agcgccatta ggcacgagga cgcgatcccc gaccttgatg
     6241 tcggaacggg taatgccttc ggctttatcg aggcttgtct gaccgggcgg cagacggcgg
     6301 taggtcgtcc gctcgtctgc cgctaccgtg acggacgagc ctgcgtcagt cttaactgtg
     6361 atctgtccgg aagcctgatc cagagccgtc acttcgccca cgagatgctg cgtctgttgt
     6421 gcggcgcggc tgacggctcc ggcggcgacg ggcgagaggt gcccgataga aacaaccacc
     6481 agcgaaatat tcaggaacgc cgaggcaata gtgtgcaatt ggttcttcat attcttcacc
     6541 gagattcttt tcatcaaaca ccgtcctgtt ttaatcagtg gcggacgagg agagcgccga
     6601 ccttagtcga tctaagtgac ggacagggca aacgtgagaa tgactgttgc tgaaaacgtc
     6661 gcgcgtggca tctgcttccc cttgctctgt aaaccatctg actgaccggg cggtggaaca
     6721 tcttgcctcg acagtttgag aagcctgcta cggttcaaaa gaaaccggct caaaacggcg
     6781 ggcaaaaatc aacgtgccta cgtttgacta agttatgctg agacttttta ccgcaacagt
     6841 ctgaggcagc ggttgtgatt ttgtctgtac taaagcgagc acaaaagaac gctttggaag
     6901 gccgcattta tgacattcgg acgggcggct ttgtggcggg agctaacgtg gcggaggcag
     6961 gagtgagtat ggagagtctg cgcatacccg gtatcgtcgc aagctcacgc cgtgggtctt
     7021 acaacaggtg actgacgcgc gcggcgcagg aagtcgccgg gatgcggctt cgtagggctt
     7081 atcgaaatga gaaacgggcg gagacgacaa ttgcgattgc cgtcatagct atctcacaca
     7141 gccgcttgac cgcgttgaag ccgccctgaa cctgagggtg gctggaaggt tagaagttgt
     7201 gcgtgtcagg ctactcgtgt caggctactc gtgtcaggct acgggttgat gctcaggcgc
     7261 gacgcggcct tcgacggcca cagttcttcc aattgccggt agtcgtcgag cacgacgatg
     7321 cggtcttcat gaaactcttc cgggcggttc gggtagatgc tgacgacggg cgcgtcgcgc
     7381 aagcccggtt cttcgcggag acggtagatc aggttgccga gccacggcag gtcggcgttg
     7441 gtgagaataa agtagggctg ctcgcgcctg gcgacctcca acgccccggg cgcatcctcg
     7501 gccttgacga cggtgtagcc gagcgcctcc agcttacgcc ccatcgactg gcgaatctcc
     7561 tgatcgtcat ccacgacgag gacgggcgaa ccggcggtca cttcgggcgc atggttaagt
     7621 tcgtcgatca tccatgcgac gcgctcgatg tccgacgccc tgccgaggaa gatcgcggag
     7681 ggcacatagt aaagatttct acgcgctttg ctgttgagga cgacaaccgg gtgagttcgc
     7741 agcgcgtcgc tgagaatggc gggagaaatc tgtccccgat tgtactggca catgccggtg
     7801 acgggtaagt gcgggatcaa gtcattaagc ctcgcctcgt attcagccgc acgctcgcaa
     7861 cccggcgctt cgcagtctat ccagcccatc tctccaatac ttctcaatcc gctgaagccg
     7921 gaagcgatgg cttgcctgac atattcgcct agccgggtga gccgctcatc gggttcaaag
     7981 tggccggaag gcaggtacgc ttcccggctg gtggagaagt cgagcgcacc gcgcgcccgc
     8041 tctctttgga catcaatccc ggcggatgtt aaaagagagg ccgcctccgc gaccgtgtgc
     8101 tc
//
LOCUS       DASWNS010000107        20016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_212189, whole genome shotgun sequence.
ACCESSION   DASWNS010000107 DASWNS010000000
VERSION     DASWNS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20016
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212189"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VGB76_03595"
     CDS             <1..435
                     /locus_tag="VGB76_03595"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY0098019.1"
                     /translation="TTTGSVLGAAGGYWLAGFVGGSWAERFQNGRVQRFLSTHSSFIA
                     LFGLRLAPSPHSVVNYAAGLARIPPTRFLLATLVAMLIKSYVYATAVQNAVGAGSGAN
                     AVSATVVLSLFGVAALSLLGHVLTRRYLSLPVAPTSVQNRLG"
     gene            591..2729
                     /gene="bchI"
                     /locus_tag="VGB76_03600"
     CDS             591..2729
                     /gene="bchI"
                     /locus_tag="VGB76_03600"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0010007 - magnesium chelatase complex
                     [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HEY0098020.1"
                     /translation="MSPPRRRVYPFASIVGQEELKLGLLLNVIAPSIGGVLIMGHRGT
                     GKSTAVRALAELLPPLARVADCLYGCDPSPETDLCADCRARLAATGKLAGKRAPVPVV
                     ELPLNATEDRVAGTINFERAITEGVKSFEPGLLARAHRGFLYIDEVNLLEDHLVDLLL
                     DASASGRNRVEREGVSIEHPSEFVLVGSGNPEEGELRPQLLDRFGLYTEIRTAADLEE
                     RVEIITRREAFDRDPEIFCALWEREQAQLRRRLTRARSGVRKVRLPDAIVRAIAQLCQ
                     RLDVDGHRGELTIARASRALAAFEGRREVTREDVRRVAAMALRHRLRRDPLEQTGGGA
                     QIEQAAGELFGAGEEATPERGSASSDARQRQPKKSPPDEPGGENNGGGTLRPAPQREE
                     AQREEARRREARREANAPNTSPARHEAQPAPPLDSRLPSQAFDEPLRLKRSEAASNTH
                     TKRRAHARTDSSRAARGRYARAVAMRTGDLKIALDATVRAAASSQMFRRSASTVRDAQ
                     AAGVERALRIETEDFRYKRFSRRTGTLYVFAVDTSGSMALNRIAQAKGALARLLRQSY
                     VNRDRVSLVTFREREGRVLLAPSQSSALAKRLLDALPVGGATPLSAGLLCALEVCERA
                     ARQGTGRIVLLVFTDGRANVPRDGRTGGDLADARRRIKSEVEKIGLALKRLGVAAVVV
                     DTQNRFTSGGEGQTLAHTLGGRYAHLPSWQ"
     gene            2953..4704
                     /locus_tag="VGB76_03605"
     CDS             2953..4704
                     /locus_tag="VGB76_03605"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEY0098021.1"
                     /translation="MSETLNQIRLTKDERDRRVLELCEQVSEIEGRLIPTGLHVFGRA
                     SVETERADLLRMVASFDRPEVGARALPQLIEENLPPDAASASDETTSAGEGSLRARER
                     IEAITRKAIERFLADGTQGACAWLEATARVPQADSRPLFALLEKIAAQLEVNRELDAF
                     VRALRGEYIEPGPGADIVQNPAILPTGRNTHAVNPYGVPSDLAFARAERVVNLLLERH
                     RTEHGRYPAAMALVLWGLDNIKTQGEGVAQALWLLGVRPVRDKMNRVTDVEAIPLDRL
                     GRPRIDVVMTVSGIFRDLFGATMGLLDRAVHTVAALDEPPGSNYVRRNIHAQMEREAC
                     AFEEAALRVFSNAAGNYGTNVNFMVMDSQWEADDTLAELFVTRKCFAYGRDREGRALE
                     GREARGAFNSALSRVEATYQNIDSFEIGITDVDHYFEYLGGVSKAVEKQSESRPAIYL
                     SDALSREAKVRSVEETIRLETRTKTLNPKWYEGMLKHGFRGVAEIESHVANTFGWSAT
                     ADAVDDWVYAEVAQTFVLDERMLERLRELNPHSARALVARLLEAEGRGFWEAEAEMIE
                     QLREIFNALEDRIEGVA"
     gene            4730..5239
                     /locus_tag="VGB76_03610"
     CDS             4730..5239
                     /locus_tag="VGB76_03610"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEY0098022.1"
                     /translation="MFARKLMGFMFCLSLTCGGVVVVQAQDVRDADKPAAISPERRAD
                     IGRLLELMNVFEEEKNSVEPLFENFKRAAPHVPAEVWRDLRKEFDAEFTKEFLLNSYL
                     PIYARRFSAAEVKEFIKFFDSPAGRKMIATLPLIREEAFAIGYERGRKLGERIREKLR
                     ARGYSLSET"
     gene            5304..7610
                     /locus_tag="VGB76_03615"
     CDS             5304..7610
                     /locus_tag="VGB76_03615"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEY0098023.1"
                     /translation="MKITVLYVGSSLLAPLRQAEREIERAHALNLKVAAYNFGAPLDE
                     PQWREIERELGESEVVFVIHVTDGENAARLIPLLEKYRSEQRAVVVINCMPDLMRQTR
                     MGRLRFGREAARAEAGVEDVATAEGGRTTAGANEGMARRLVGRIGAWMGEQARARRGG
                     RGGSHTQYLRYVERLPALLRVVPNAGRLRDAKNYLSLFCYFLQPTPANIRSMLLLALK
                     NYVADERLEKIELAPPESMPAVAVYHPDAPALFETFESYRVWYERRAGRNGGAKLDPD
                     KTIGLLLMRPQIVSGAHAHYDALIRAIEAEGLATVPAISTFMDNREACEMFFVEKQAS
                     PVARRALRLSGRRRLPGESKTTGGGERTKGKSRVSQIVSLTGFSFVGGPAMNDAEAAV
                     EFLRELNVPFRSAVSLDVQTIEAWRESWTGLNPVQAGMQIAIPEIDGATEPFVYGGMS
                     QASASPLPLEDRCERIARRLAKWNRVQCAPRAEIKVALVVFCFPPNKGNIGTAADLDV
                     FPSVWEMLKRLEREGYRVEVPESADELRERLLGGNADSFGATANVAYRMTVEEYRRLC
                     PFAREIEAEWGSAPGAINSFGGTLLIEGLQLGNIFIGVQPTFGYEGDPMKMLMARSGA
                     PHHGFAAFYAFIEKVFRADTVAHVGTHGALEFMPGKQVGLSESCWPDRLIGELPNVYI
                     YSVNNPSEGSIARRRSYAELISYLTPPVEDAGVYRELASLKELLLAYRQTTDEQEREQ
                     LYQSIDEKAAALHLLTGEAHAQRELSAR"
     gene            complement(7870..8151)
                     /locus_tag="VGB76_03620"
     CDS             complement(7870..8151)
                     /locus_tag="VGB76_03620"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY0098024.1"
                     /translation="MEDANKIHDRRKAQVTLYTRPGCHLCDEAKQAMRAARCEGQYTL
                     REINIDLDPALVRRYGWDIPVVLIDGIETFRHRLTPSEFEREIRRAVFH"
     gene            8455..9564
                     /locus_tag="VGB76_03625"
     CDS             8455..9564
                     /locus_tag="VGB76_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0098025.1"
                     /translation="MDSEKDPNNLGGSWTGKTPADETQGSTSAPAEAGGAESAASSSD
                     ETADERRARLMAEVKAKAAARMAAKGGAPAKPETPAATPAAPPVTTPAANADAPSGET
                     PEEKRARIIAEAKAKAAAKMAAKGSPTAGSEAATTTPTAATTTPAATTTVDVPDAPGR
                     DVPSGTPPATRVAPGAAKATPTASVAQGVPAAVAARQHPTTVDTIPEPPINKMRRRII
                     WASIIGFVGTSLLMFFRFFLPRTIFEPSSIFRVGFPGDYGVGVDTKYQQLYRIWVVKT
                     SDRLFVINAVCTHLGCTPDWKASENKFKCPCHGSGYDSEGINFEGPAPRPMDRAHIEL
                     DAEGQIVVNKGRLYDWPKGGENRFEEPGAYIPMGG"
     gene            9698..10753
                     /locus_tag="VGB76_03630"
     CDS             9698..10753
                     /locus_tag="VGB76_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY0098026.1"
                     /translation="MDSERPDEKEGQPKTGNGSSQTPAEKEDIKSLLRAASGVPEGGH
                     KALSAETTTPEGKRAAGAPGVGLIERTKEQGLALKERALDEAKTYKDPQQTQLWKSIF
                     RVSHDRSDPRNRSLAILSNVFLHLHPAKINRDAVRYGFTWGMGGITFYLFIVLTFTGV
                     LLMFYYHPTKVQAFRDILYLEHDVPFGKLLRNMHRWAAHLMVITTWLHMFRVVLTGSY
                     KRPREFNWCVGVVLLVLTLLLSFTGYLLPDDQLGFWAVTVGTNMARATPMLGSEGPFG
                     PQLGMTPYNDVRFGLLGGSIVDANALLRSYIWHCIGIPLVASIFMAVHFWRIRKDGGI
                     SGPAPVMLESEMKATKK"
     gene            10836..11771
                     /locus_tag="VGB76_03635"
     CDS             10836..11771
                     /locus_tag="VGB76_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098027.1"
                     /translation="MDWHQLWIITSAPDNVPIVGMLFLVPFFTWYGLKQARENDKLIV
                     ELEGDTAKAKTHHRKTLPWRPGWARELHVWPFLVRVEFLAAVIVTVILFIWSITLDAP
                     LEEPANPNLTMNPSKAPWYFLGLQEMLVYFDPWIAGVVMPSIMMIGLMVFPYVDSNPL
                     GNGYYTIRQRKFALGMFGIGWFTWIILIVIGTFIRGPGWVWFWPGQTWDHNAVVFDKN
                     RDLHDLFGITSMPVKIIFGAIVVGLFYLLAGLFLHWLMTRNEFERKLLARTSLLQYLT
                     FQFFAISVLIALPVKILLRLAFTIKYVWVTPWFNV"
     gene            11798..15550
                     /locus_tag="VGB76_03640"
     CDS             11798..15550
                     /locus_tag="VGB76_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0098028.1"
                     /translation="MAETPKTDTDALAKREPETPARDPIISRSTSNILLICALLLMAS
                     LAWALYDEAYGQRPWKGMQQEFVSRYNRYLKRLKRTGTQSEKDIKATDEYKELERAVS
                     EANQAAAPRKKEIDSEVKFIDRQLAAITDPFQNKRGYITVVNYRIETSDESDRADLRK
                     EVEEQKQEKVDVEMPVDPGSDKTQEQKLNYTELEAKYLALKEQKAKLLAERAEVTKEA
                     DELAKQRDTYLKDNIIGLSAEQIDKLIESNEKFDYSLRQINVNGDQLVDRCETCHLGV
                     RQPLELKAVNFKRPGKKLDASARAFVSHPSKELLQIHDPERFGCSSCHGGNGRATTSV
                     VKGHGRHRFWLHPLHERENMEAGCQQCHAQDRVLQGANVLTQGKDLFQERGCVGCHRY
                     EGFDRETDGLANSRQNIKQLEEELAANDREARQLDVEAGTVTDETEAQEKLARAVSLR
                     VTNSQIEARIEQLNVQARYLMQDQKKVGPNLKDVRLKLRKEWIPAWLKDPQGFRPGTK
                     MPTFWYLNPEHELSKNFKQNADNERAAIAAYLWQSSFTGQVPKQEPGDATHGKELFET
                     KGCMACHSLGEGVENQVGGTFAANLQRVGEKADFNYIVRWIYNPRERWAPYCPKEKRD
                     LTREDYEKNGKPYRFDTEGHSKCPNDGAELQVQNMTVMPNFRLTEQDSRDIATYLFSL
                     SKGTQWPDASFMDDPKLKDQGAYYIKQYGCANCHEIRGFEDEQRIGKELTAEGATPIE
                     RLDFALLTHDAEKNINPFTEKKYERREGMQESWYNRKGFFEHKLEEPGIYDKGKEKEP
                     RDHLRMPDPYLTPEWKNALTTFLLGSVGAEGANVPTSFFYQPTDRRKDIQDGWWVIKK
                     YNCMGCHSVQVGQKSVLQDLPQYLTPEGKDQLPPALMTEGARVDPNWLLRFLTDPSLS
                     NGSDDPTVRGLTGRNNGGQSGSRASGASGGNGQTSGGGGSGAGGGEASSNQTQSGGAT
                     AASGGNSSGGNSSGASQSNDAAPELPPQPGENKNGVRLYLKARMPTFNFSPNELRTLV
                     RFFLAVSSQQEPFIKEPVDPLTDQERNLARQLFTSQQAPCLKCHLTGNPEHDKNASAP
                     NFLQAGERLKPGWTFRWLLDPQKVIPGTAMPSELFKRDGERWVFNGELPESFNEYKGD
                     HADLLVRYMLQLTPAEQARLASGSPAATGGTTTTGESKNAAAQSSTHHARARAKAAAR
                     NRKYRLRAAASRAPQGSRRSVRRVREPVVSVRQYGVGYSPPHGRSW"
     gene            15587..16474
                     /locus_tag="VGB76_03645"
     CDS             15587..16474
                     /locus_tag="VGB76_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0098029.1"
                     /translation="MPFKQLSRLLALMLAAAMLSFMLACGGDDDDDVAEEDGAATEAA
                     AGTPYKSSGNEGSITGAVNFTGEPPAPKPISMDADAACAQNNANPQTEDVVVKDGKLA
                     NVFVYIKDGKTTEGKSITSFAFEVPATEVTLDQKGCRYIPHVLGIMAGQKLKVLNSDP
                     TAHNVHPSPKSNKEWNQSQPAGAQPIIQSFSRPEVVVPVKCNQHPWMKANIGVLRHPL
                     YAVSGENGAFEIKGVPPGTYTVATWHERYGEKTQSVTVGAKETKTQDFAFDASAGKTA
                     IHGSLEVLPAIEFPVITKH"
     gene            16772..17701
                     /gene="cyoE"
                     /locus_tag="VGB76_03650"
     CDS             16772..17701
                     /gene="cyoE"
                     /locus_tag="VGB76_03650"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY0098030.1"
                     /translation="METIVVEEIEQSVAPGAVEGGRSATREKLSAYYELTKPRITFLV
                     VLTTSAGFLLGSTRGAFDYQLFAHTLLGVALLCSGISTLNQWMERGLDGLMRRTARRP
                     LPLGTLSPRAALIFGLSLTVGAEAYLAVFVNLLSAFCGLTVIVGYLLCYTPLKTRTSL
                     STLVGAFPGAVPPLLGWAAATNHVGLEAWALFAILFLWQFPHFFAIAWMYREDYARAG
                     ILMLPVVEPEGRLTRQQIVVWTLMLVPVSLAPAALGISGAIYFFGALVLGALFLWSSI
                     AAAVTLSRQSARRVLLASVLYLPVLFVLMVINQ"
     gene            17775..18653
                     /locus_tag="VGB76_03655"
     CDS             17775..18653
                     /locus_tag="VGB76_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY0098031.1"
                     /translation="MGRALGVLIWLLTFGSVWLFVNGRWWLPPYISEHGPAYDRQFLI
                     TIIVVGVSFVAAQIGLGWAVWRYGSKGDRTERAVYSHGNNRLEIIWTAITALIFITLG
                     LMGQRVWAQLHLNEAPAGSTRVHVVAQQFQWNFHYPGADGTFGRTDPKFVKDAELNFV
                     GLDEQDQNAKDDQVVPALAVPVGRPIELTLQSKDVTHDLFVPELRYKQDVVPGMAIKM
                     HFTLTKEGRFELACAELCGQLHYKMKSYLIAIPASAYDELVALPQAQFQARITQLIKE
                     YPLGIRQATPAAAASR"
     gene            18697..>20016
                     /locus_tag="VGB76_03660"
     CDS             18697..>20016
                     /locus_tag="VGB76_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0098032.1"
                     /translation="MEEATTPFEPHAATATAAGAVDDAAAAASPHAAEAAHHHGAPQG
                     FIRKYIFSIDHKVIGIQYLLLAIFAAFVGMFMSVLMRLNLTWPGRKWTILETLFPLGA
                     PGGVMSPEFYLSLVTMHGTMMVFFVLTTAPQGGFGNYFLPIQVGAEDMAFPVLNMLSF
                     WVTFVGLVVLIAAIFVEGSTIIGNTDFVNNYLGGGGTGTLGPIGGWTGYPPLSALGKI
                     AGPGQGAGATLWITSIAIFCVASLLGALNFITTLINMRAPGMTLMRMPLTCWSWFTTA
                     ILALLAFPVLLAAGVLLLLDRHAGTSFFIPGGLVFSDAVLPNKGGSPILWQHLFWFFG
                     HPEVYIAILPGMGATSHILSTFARKPVFGYRAMVFAMFAIGLLGFFVWGHHMFISGMD
                     PRTSIAFSVLTLSIGVPSAIKTFNWLGTLWGAKIRFTVPMMFAIGFVS"
BASE COUNT         3983 a         6159 c         6178 g         3696 t
ORIGIN      
        1 acgacgacgg gcagcgtgct cggcgcggcg ggcggctatt ggctcgcggg ctttgtcggc
       61 ggctcctggg cggagcgttt tcagaacggg cgcgtgcagc gtttcctgtc cactcattcc
      121 agtttcatcg cgctcttcgg cctgcggctc gcgcccagcc cgcacagcgt ggtgaactac
      181 gcggcgggtc tggcgcgcat cccgcccacg cgattcctgc ttgcgacgct cgtggcgatg
      241 ttgatcaagt cttacgtcta tgcgaccgcc gtgcagaacg cggtcggagc gggttcgggg
      301 gcaaacgccg tgagcgcaac agtcgtgctc tcgctcttcg gcgtggccgc gctctcgctc
      361 ctcggtcatg tcctgaccag acgctacctg tcccttccgg tcgcgccgac ttcggtgcaa
      421 aatcgtctcg gctgaacacg cgaccgtcga gcttgacggg tagataaagt ttcgtctctg
      481 acaagcgttc gcgccggatg ctccggcaaa ctcaccaaat tgacttcaaa gcaacgccaa
      541 ctgcgagcat gacccgacgg cctgacactt cccgacagcg agacgcaaca ttgtcgccgc
      601 cccggcgacg ggtctatccg ttcgcgtcca tcgtcgggca ggaggagttg aaactcggat
      661 tgctcctgaa cgtcatcgcg ccttccatcg gcggcgtctt gatcatggga catcgcggca
      721 cggggaagtc aaccgccgtg cgcgctctgg ccgaactctt gccgcccctc gcgcgcgtgg
      781 ccgattgtct ctacggctgc gatccgtcgc cggaaacaga cctgtgcgcc gattgccgtg
      841 cgcgcctcgc cgcgacgggc aaactggcag gcaagcgcgc gcccgttccc gtcgtcgagt
      901 tgcctctgaa cgcgaccgag gatcgcgtcg cgggcacgat caactttgaa cgcgcgatta
      961 cggagggcgt caagagtttc gagccggggc tgctcgcacg cgcgcatcgc ggttttctct
     1021 acattgacga agttaattta ctcgaagacc atctcgtgga tttgctgctc gacgcgtcgg
     1081 cttcggggcg caatcgcgtc gaacgcgaag gcgtttcaat tgagcatccg tcggagttcg
     1141 tactcgtcgg ctccggcaac ccggaggagg gcgaactgag gccgcaactg ctcgaccgct
     1201 tcggcctcta cacggagatt cgcaccgccg ccgacctcga agagcgcgtc gagatcatca
     1261 cccggcgcga agccttcgac cgcgacccgg agatattttg cgcgctctgg gagcgtgaac
     1321 aggcgcagct tcgtcgtcgt ctgaccagag cgcgaagcgg cgtccgaaaa gttcgactcc
     1381 cggatgcaat cgtgcgcgcc atcgcgcagc tatgccagcg gctcgacgtt gacggacaca
     1441 ggggagagtt gacgattgcg cgtgcgtcgc gtgcgctggc cgcgtttgaa ggtcgccgcg
     1501 aggtgacgcg cgaagacgtg cgccgtgtgg ccgcgatggc tctgcgccac cgcctgcgcc
     1561 gcgacccgct cgaacagaca ggcggcggcg cgcagatcga acaggccgcc ggagaactct
     1621 tcggcgcggg cgaagaggca acgcccgaac gcggctccgc ttcaagcgac gcgcggcaac
     1681 gccagccgaa aaagagtccg cccgatgagc cgggcggaga gaacaacggc ggaggaactt
     1741 tgcgacccgc gccgcaacgc gaagaggcgc aacgtgaaga ggcgcgacgc agagaggcgc
     1801 gacgcgaagc gaatgcgcca aacacctcgc ctgcgcgaca cgaagcgcaa cccgcgcctc
     1861 cgctcgattc gcgtctccct tctcaagcct tcgacgagcc tttgcgcttg aagagatcgg
     1921 aagcggcttc aaacacgcac acgaaacggc gagcacacgc gcgaacagat tcgagccgcg
     1981 cggcgcgtgg gcgttacgcg cgcgccgtcg ccatgcgaac cggcgacttg aagatcgcgc
     2041 tcgatgccac tgtgcgcgcg gcggcttcgt cgcagatgtt tcgccgtagc gcgagcaccg
     2101 tgcgcgacgc tcaggccgca ggcgttgagc gcgcgcttcg gattgagacg gaagattttc
     2161 gttacaagcg attcagcagg cgcaccggca cgctctacgt ctttgccgtt gacacttcgg
     2221 gcagcatggc gctcaatcgc atcgcgcagg cgaagggcgc gctggcgcgt ctgctcaggc
     2281 agtcttacgt caaccgggat cgcgtctcgc tcgtcacctt ccgcgaacgc gaggggcggg
     2341 tgttgctcgc tccgagtcaa tcgagcgcgc tggcgaaacg tctgctcgac gcgctcccgg
     2401 tcggcggggc gacgcccttg agtgcgggct tgctgtgcgc gctcgaagtg tgcgagcgcg
     2461 ccgcgcgaca gggcaccggg cgcatcgtcc tgctcgtctt cacggacggg cgcgcgaacg
     2521 ttccgcgcga cggtcggaca ggcggcgacc tcgcggacgc gcggcgacgc atcaagagtg
     2581 aagttgagaa gataggcctt gccttgaaac gcctcggcgt cgcggcggtg gtcgtggaca
     2641 cgcagaaccg tttcacctcc ggcggcgaag gacagacgct cgcgcacacg ctcggcggtc
     2701 gctacgcgca tctcccttcg tggcaatagc gacattctcg tttacaaaga gaatcaaacg
     2761 tggctatttt atctccgccg tttaagcgtc agtcttaaac tccatttttg cgcctctctt
     2821 ctttgcgcct ccaacgtgag tttattcttt agacagcatt agtctcacgc aaaagcgcac
     2881 agggaagaag gctgagaatt ttttgcagcg aactcgaaat ctctccggcc tgtcgcggcg
     2941 aactgtcaga acatgagcga gacactaaac cagattcggc tcacgaagga cgagcgcgac
     3001 cgtcgcgtgc tcgaactctg cgagcaggtg tcggagatcg aggggcgtct gatccccacg
     3061 ggcttgcacg tcttcgggcg cgcttccgtc gaaaccgaac gcgcagacct cttgcgcatg
     3121 gtcgcctctt tcgacaggcc ggaagtcggc gcacgcgcgc tcccacaact catcgaagaa
     3181 aatctgccgc ccgacgctgc ttccgcgtcg gacgagacga cgagcgcggg cgagggaagt
     3241 ctgcgtgcgc gcgagcgcat cgaagcgatc acgcgcaaag ctatcgagcg ttttctcgca
     3301 gacgggacgc agggcgcgtg cgcctggttg gaagcgacgg cgcgtgtccc gcaggccgac
     3361 tcacgccctc tctttgcgct gctggaaaag atcgcggcgc agttggaggt caatcgggaa
     3421 ttggatgctt ttgtgcgtgc gctgcgcggc gagtacatcg agccgggacc gggcgcagac
     3481 atcgtgcaga atccggccat cctgccgacg gggagaaaca cgcacgccgt caatccttac
     3541 ggcgtccctt ccgatctcgc cttcgcgcgc gccgaacggg tcgtcaatct cctgctcgaa
     3601 cgccatcgca cggagcacgg acgctacccg gcggcgatgg ctctcgtcct ctgggggctg
     3661 gacaatatca agacgcaggg cgagggcgtg gcgcaagccc tgtggctgct cggcgtgcgc
     3721 cccgtgcgcg acaagatgaa tcgcgtcaca gacgttgaag cgatcccgct cgacagatta
     3781 ggcagaccgc gtattgatgt cgtgatgacc gtctcaggca tcttccgcga tctcttcggc
     3841 gcgacgatgg gactgctcga ccgcgccgta cacacggtcg ccgcgctcga cgaaccgccc
     3901 ggttcgaact acgtgcggcg caacattcac gcgcagatgg agcgggaggc ttgcgcgttt
     3961 gaagaggccg cgctgcgcgt cttctccaac gcggcaggca attacggcac aaacgtcaac
     4021 ttcatggtga tggattcgca gtgggaggcg gacgacacgc tggccgagtt gttcgtcacg
     4081 cgcaagtgtt tcgcctacgg gcgcgaccgt gaagggcgcg cgctcgaagg acgcgaagcg
     4141 cgcggcgcat tcaacagtgc gctctcgcgc gtggaagcga cctatcaaaa catagactcc
     4201 ttcgagatcg gcatcacgga cgttgatcac tatttcgaat atctgggcgg cgtctcgaaa
     4261 gcggtggaga aacagtcgga gtcgcgcccc gcgatctacc tgtcggacgc gctctcgcgc
     4321 gaggcgaagg tgcgttcggt ggaagagacg atccggctgg agacgcgcac gaagacgctc
     4381 aatccgaagt ggtatgaagg catgctcaaa cacggcttcc ggggcgtggc cgagatcgaa
     4441 agccacgtcg ccaacacgtt cgggtggagc gcgacagccg acgcggttga cgattgggtc
     4501 tacgcggaag tggcgcaaac tttcgtgctg gacgagcgga tgctcgaacg cttgcgcgaa
     4561 ctcaatccgc attcggcgcg tgcgctcgtt gcgcgcctgc tcgaagccga ggggcgcggt
     4621 ttctgggagg ctgaggcgga gatgatcgaa caactgcgag agattttcaa cgcgctggaa
     4681 gatcgcattg agggcgtcgc ctgaacttga agggtaaaag gagtaagaga tgttcgctcg
     4741 caagttaatg ggatttatgt tctgcctgag tcttacctgc ggcggcgtcg tcgttgtgca
     4801 ggcgcaggac gtgcgcgacg ccgacaagcc tgccgcgatc agcccggagc ggcgggcgga
     4861 tatcggtcgt ttgctggaac tgatgaacgt cttcgaagaa gagaagaact cggtcgagcc
     4921 tctgtttgag aatttcaaac gcgccgcgcc gcacgttccg gcggaagtct ggagagattt
     4981 aaggaaagag tttgatgccg agttcactaa ggagttcctc ttgaactcct acttgccgat
     5041 ttacgcgcgc agattcagcg cggcggaagt gaaggagttc attaaattct tcgattcccc
     5101 ggcgggcaga aagatgatag ccacgctgcc gctcatcaga gaggaagcgt tcgccatcgg
     5161 gtatgaacga gggcggaagt taggcgagcg catacgagag aaactgcggg cgagaggcta
     5221 ctccctgtcc gaaacttaaa agaacagaac acgacgaacg cgctgccgcg tcgtcgctcc
     5281 actccaaagc tgaggtcgaa atcttgaaga tcacagtcct ctacgtcggc tcaagtttgc
     5341 tcgcgccgct caggcaggcg gagcgcgaga tcgagcgcgc gcatgctttg aatttgaagg
     5401 ttgcggctta caacttcggc gcgccgctgg acgagccgca gtggcgcgag atcgagcgcg
     5461 aactcggcga gtcggaagtg gtcttcgtca tacacgtgac ggacggggag aacgccgcgc
     5521 gcctgatccc tctgctcgaa aagtacaggt cggagcagcg ggcggtcgtc gtcatcaact
     5581 gcatgcccga cttgatgcga cagacgcgta tggggcgttt gcgcttcggg cgcgaggccg
     5641 cgcgcgcgga ggccggggtt gaggatgtgg cgacagctga aggcgggcgc acgacggcgg
     5701 gcgcaaatga agggatggcg cggcgtctgg tgggtcgcat cggcgcgtgg atgggagagc
     5761 aggcgcgtgc gcggcgcggg ggcaggggcg ggagccacac acagtatttg cgttacgtag
     5821 agcgtctgcc agccctgttg cgcgtcgtgc cgaacgcggg acgcctgcgc gatgcgaaga
     5881 attatctctc gctcttctgc tactttctgc aacccacgcc cgccaacatc cgctcgatgt
     5941 tgctcctcgc gctgaaaaat tacgtggccg acgaacggct ggaaaagatc gaactcgcgc
     6001 cgcccgaatc catgcccgcc gtcgccgtct atcacccgga cgcgcccgcc ctgtttgaaa
     6061 cgttcgagtc ttatcgcgtc tggtacgaac ggcgcgcggg tagaaacggc ggcgcgaaac
     6121 tcgatccgga caagaccatc ggcctgctcc tgatgcgacc gcagatcgtg agcggcgcgc
     6181 acgcgcacta cgacgccctg attcgcgcca tcgaagccga agggctggcg accgtgcccg
     6241 cgatctcgac cttcatggac aaccgcgaag cgtgcgaaat gttctttgtc gaaaaacagg
     6301 cgtcgcccgt cgcacgtcgc gcgttgcgcc tgtccggcag gcggcgtctg ccgggagagt
     6361 cgaaaacaac cggcggcggg gaacggacga agggcaagtc gcgcgtgagc cagatcgtgt
     6421 cgctgacggg gttcagcttc gtcggtggtc cggcgatgaa cgacgcggaa gcggcggtcg
     6481 aatttttgcg cgaattgaac gtgccgtttc gttccgccgt gagtttggac gtgcagacga
     6541 ttgaggcgtg gcgggaatcg tggacgggtc tgaatccggt gcaggccgga atgcagatcg
     6601 ccattccgga gattgacggc gcgaccgagc ctttcgtcta tggcggcatg tcgcaggcga
     6661 gcgcgtcgcc gctcccgctc gaagatcgct gcgaacgcat cgcgcggcgg ctcgcaaaat
     6721 ggaatcgtgt gcagtgcgcg ccgcgtgcag agatcaaggt tgcgctcgtc gtcttctgct
     6781 ttccgccgaa caagggcaac atcgggacag ccgccgacct cgacgtgttt ccgagcgtgt
     6841 gggagatgct caagcgtctg gagcgggaag gctaccgcgt cgaagtgcct gagagcgcgg
     6901 acgagttgcg cgaacgactc ctcggtggta acgccgactc gttcggggcg acggcgaacg
     6961 tggcttaccg gatgacggtc gaggagtaca ggcgtctgtg cccgtttgcg cgggagatcg
     7021 aagcggagtg gggcagcgcg cccggcgcga tcaactcttt cggcgggacg ttgctcatcg
     7081 aagggttgca actgggcaac atcttcatcg gcgtgcagcc gactttcggt tacgaaggcg
     7141 acccgatgaa gatgttgatg gcgcgaagcg gcgcaccgca tcacggcttc gcggctttct
     7201 acgccttcat cgaaaaagtt tttcgggcgg atacggtcgc gcacgtcggc actcacgggg
     7261 ctttggagtt catgccgggc aagcaggtgg ggctgtccga aagttgctgg ccggatcgtt
     7321 tgatcggcga gttgccgaac gtgtacatct acagcgtcaa caatccttcc gagggttcaa
     7381 tcgcgcgcag gcgttcctat gccgagttga tctcatacct gacgccgccc gtggaagatg
     7441 cgggagtcta tcgcgaactc gcttcgctca aggaactgct gctcgcctac cgccagacga
     7501 ccgacgagca ggagcgcgaa caactctatc aatcaattga cgagaaagcc gccgcgctac
     7561 atctcctgac cggagaggcg cacgcgcaga gagagcttag cgcacgttga acatgtcttg
     7621 tcccgtgtcg caagttttca acggctttgc atgacctgcc ggaagtctgt ttcgacggcg
     7681 cgcaggcgag cgcgccagca agccgctcac aacctgagaa tggtcttgat agcaaagttc
     7741 atcttctcct aatcttccgc ctcgcaaccg tcagcagcat ttcttgagac agacgcaaca
     7801 caggctctta cgtctgcggc cacacggctg tctgcctgcg actggttggc gggggacttc
     7861 tgctatctct taatgaaaga ctgcgcgccg gatttcacgc tcgaattcag atggcgtgag
     7921 ccggtgcctg aaagtttcga tgccgtcgat gaggaccacg ggaatgtccc atccgtagcg
     7981 gcggacgagt gcggggtcga ggtcgatatt gatctcccgc agcgtgtatt gaccttcgca
     8041 gcgggctgcg cgcatggcct gtttcgcttc gtcacagagg tggcagccgg ggcgcgtgta
     8101 gagcgttacc tgcgccttgc gccggtcgtg aattttattt gcgtcctcca ttttcgccct
     8161 ctccggcgac gagcgcaccg cgtcgtcgtc gcgaaatttc tcaaaaaata catcttgcca
     8221 caatggcgtg aaaagagtat aaaaggcgaa cacgccgggt cgttttacta taattttatt
     8281 tgacgcggtg gctcttctaa gtaacgcata gcatcacaat ccctgacgcg gagaggtcac
     8341 tcctctttct gggagagtcc taccaatctc tccgtcaagg cttatcaagg ggtttgtcta
     8401 tctccagaac gtgtgagcgt gcccgcctca catttcctaa cacggaggtt gcgtttggac
     8461 agcgaaaaag accctaacaa cttaggcggc agttggacgg gcaagacgcc cgccgacgag
     8521 acgcaaggct cgacctctgc gcccgcagag gcaggcgggg cggagtctgc cgcgtcatcc
     8581 tctgatgaaa ccgccgacga gcgacgcgcg cgtttgatgg ccgaagtgaa agccaaggcc
     8641 gccgcccgga tggctgcgaa aggcggcgcg cccgccaagc ccgaaactcc tgcggcaact
     8701 cccgccgcgc cgcccgtgac gacgcccgcc gcaaacgctg acgctccctc aggcgaaact
     8761 cccgaagaga aacgtgcgcg catcatcgcg gaagcgaaag cgaaggcagc cgccaagatg
     8821 gccgcgaaag gcagccccac cgccggaagc gaagccgcga ccacgacgcc gacagccgcg
     8881 accacgacgc cagccgcaac aacgaccgta gatgtcccgg acgctccggg cagggacgtg
     8941 ccgagcggca cgccgcccgc cacgcgcgtc gcaccgggcg cggccaaagc gactccgacg
     9001 gcgagcgtcg cgcagggcgt ccccgccgcc gtcgccgcga ggcaacaccc gacgaccgtt
     9061 gacaccattc ccgaaccgcc catcaacaag atgcgtcgtc gcatcatctg ggcgagcatc
     9121 atcggttttg tggggacgag cctgttgatg ttcttccgct tctttctgcc gcgcacgatc
     9181 ttcgagcctt cgagcatctt tcgcgtcggc ttccccggcg attacggcgt gggcgtggat
     9241 acgaagtatc agcagttgta ccgcatctgg gtcgtcaaaa cttctgaccg cctgttcgtc
     9301 atcaacgccg tctgcacgca cctcggctgc acgcccgact ggaaggccag cgaaaacaag
     9361 ttcaagtgtc cctgtcacgg cagcggctac gacagcgagg gcatcaactt cgaaggcccc
     9421 gccccgcgcc cgatggatcg cgcgcatatc gaactcgacg ccgaagggca gatcgtcgtc
     9481 aacaagggca ggctctacga ttggccgaaa ggcggcgaaa acaggtttga agaacccggc
     9541 gcatatatcc ctatgggtgg atagcgtccg gcgtctgctt caaagcttca atcaacaatt
     9601 tcttgccggt tcacatcaag cgggtcttca gtcacttttt aacagttcac gtgtcggcgg
     9661 gcttagcgtt taagccctaa gagtaggaga gacagggatg gattctgaac gtcctgacga
     9721 gaaggaaggc cagccgaaaa cgggcaacgg ttcgtcgcag acgcccgccg aaaaagagga
     9781 catcaagtcg ctgctgagag ccgcgtccgg tgtgccggag ggcggccata aggctctctc
     9841 cgctgaaacg acgacgcccg aaggcaagcg cgccgccgga gcgccgggcg tcggcctgat
     9901 cgagcggacg aaagagcagg ggcttgcgct caaggaacgc gcgctcgacg aagcgaagac
     9961 ttacaaagac ccgcaacaga cgcaactctg gaagtctatc ttccgcgtct cgcacgaccg
    10021 cagcgacccg cgcaaccgca gtctggcgat cctctcaaac gtcttcctgc acctccaccc
    10081 ggcgaagatc aaccgcgatg cagtgcgcta cggcttcaca tggggcatgg gcggcatcac
    10141 cttctatctc ttcatcgtgc tcaccttcac gggcgtgctt ttgatgttct actaccaccc
    10201 gacgaaggtg caggcgttcc gcgacattct ctatctcgaa cacgacgtgc ccttcggcaa
    10261 actgctgcgc aacatgcacc gctgggcggc gcacctgatg gtcatcacca cctggctgca
    10321 catgtttcgc gtcgtcctga cgggttcata caagcgaccg cgtgagttca actggtgcgt
    10381 cggcgtcgtg ctcctcgtcc tgacgctcct gctctcgttt acgggttacc tgttgcccga
    10441 cgaccaactg gggttctggg cagtgacggt cggcacgaac atggcgcgcg ccacgcccat
    10501 gctcggcagc gaaggaccct tcggacccca actcggcatg acgccctaca acgacgtgcg
    10561 cttcggcctc ctcggcggct ccatcgtgga tgccaacgcc ctgctgcgct cctatatctg
    10621 gcactgcatc ggcatcccgc tcgtcgcctc gatcttcatg gccgtccact tttggcgcat
    10681 acgcaaagac ggcggcatct ccggccccgc gcccgtgatg ctggaatcgg aaatgaaagc
    10741 aactaagaag tgatgagtga tgggtgatga gtaatgagtg aaaacaaacc actcttactc
    10801 atcactcatc atgcatcacg catcacttaa agattatgga ctggcatcaa ctctggatca
    10861 tcacgtctgc gccggacaac gtgcctattg tggggatgtt gttcctcgtc ccgttcttca
    10921 cctggtacgg cttgaagcag gcgcgcgaaa acgacaagct gatcgttgaa ctcgaaggcg
    10981 acacggcgaa ggccaagacg catcaccgca agactctgcc gtggcgtccg ggctgggcgc
    11041 gcgagttgca cgtctggccg ttcctcgtgc gcgtggaatt tctggccgcc gtgatcgtca
    11101 ccgtcattct cttcatctgg tcgatcacgc tcgacgcgcc gctcgaagaa ccggccaacc
    11161 cgaacctgac gatgaaccct tcgaaagcgc cctggtactt cctcggcctg caagagatgc
    11221 tcgtctattt cgacccgtgg atcgcgggcg tcgtgatgcc ttcgatcatg atgatcggct
    11281 tgatggtctt cccctacgtc gattcaaacc cgttggggaa cggctactac acgatccggc
    11341 aacgcaagtt tgcgctcggc atgttcggca tcggctggtt cacctggatc atcctgatcg
    11401 tcatcggtac gttcataagg ggtcccggct gggtctggtt ctggccgggg cagacgtggg
    11461 atcacaacgc cgtcgtcttc gataagaacc gcgacctgca cgatctgttc ggcatcacga
    11521 gcatgcccgt caagatcatc ttcggcgcaa ttgtcgtcgg cctcttctac ctcttggccg
    11581 gactgttttt gcactggctg atgacgcgca acgagtttga gcgaaaactg ctggcgcgca
    11641 cgagcctgct gcaatacctg acgttccagt ttttcgccat ctccgtgctc atcgcgctgc
    11701 cggtgaaaat cctgctgcgg ttggccttta cgatcaagta tgtgtgggta acgccctggt
    11761 ttaatgtctg aacccgattt cctggaggga gacgaaggtg gccgagactc ctaaaaccga
    11821 cacggatgcg ctggcgaaac gcgagccgga aacccccgcg cgcgatccga tcattagccg
    11881 ttcgacgagc aacattcttc tcatctgcgc gctgctgctg atggcctcgc tcgcgtgggc
    11941 tttgtacgac gaagcctacg gtcagcgtcc gtggaagggc atgcagcagg agttcgtctc
    12001 gcgttacaac cgttatttga agcgtttgaa acgcaccggc acgcaatcgg agaaggacat
    12061 taaagcgacg gacgaataca aggaactcga acgcgccgtg agcgaggcga atcaggccgc
    12121 cgcgccgcgt aaaaaggaga tcgacagcga ggtcaagttc atcgaccggc agcttgccgc
    12181 catcaccgat cctttccaga acaaacgcgg ctacatcacc gtcgtcaact atcgcatcga
    12241 gacttccgac gaatccgacc gggctgatct gcgtaaagag gtcgaggagc agaagcagga
    12301 gaaggttgac gtggagatgc ccgtcgatcc cggctccgac aagacgcagg aacagaaact
    12361 caactacacc gaactcgaag caaagtatct cgcgctcaaa gaacagaagg ccaagttgct
    12421 cgccgaacgc gccgaagtca cgaaggaagc ggacgaactg gcgaagcagc gcgacaccta
    12481 tctgaaagac aacatcatcg gcctcagcgc agagcagatt gataaactga tcgagagcaa
    12541 cgaaaagttc gactactcgc tcagacagat caacgtcaac ggcgatcaac tcgtcgaccg
    12601 ttgcgagaca tgccatctcg gcgtccgcca gccgctcgaa ctcaaggccg tcaacttcaa
    12661 gcgaccgggc aagaaactcg acgccagcgc acgcgccttc gtctcccatc cgagcaagga
    12721 gcttttacag attcacgacc cggaacgctt cggttgttcg agttgtcacg gcggcaacgg
    12781 gcgcgccacc acgagcgtcg tcaaaggtca tggccgtcat cgcttctggc tccacccgct
    12841 ccacgagcgc gagaacatgg aggcgggttg ccagcagtgc cacgcgcaag accgcgtgct
    12901 gcaaggcgcg aacgtgttga cgcagggcaa agacctcttc caggagcgcg gctgcgtcgg
    12961 ctgccaccgc tacgagggtt tcgaccggga gacggacggg cttgccaact cccggcagaa
    13021 catcaagcaa ctcgaagaag aactcgccgc caacgaccgc gaggcgcggc agttggacgt
    13081 ggaagcgggc accgtgacgg acgagacgga agcgcaggag aaactcgcac gcgccgtctc
    13141 gctgcgcgtc acgaacagcc agatcgaggc gcgcatcgag cagttgaacg tgcaggcgcg
    13201 ctacctgatg caggatcaaa agaaggtggg gccgaatctg aaagacgtgc gcctgaagtt
    13261 gcgcaaggag tggattccgg cgtggctcaa agacccgcaa gggttccgcc ccggcacaaa
    13321 gatgccgacc ttctggtatc tcaaccccga acacgagcta tcgaagaact tcaaacagaa
    13381 cgccgacaac gaacgcgccg ccatcgccgc ctatctgtgg cagtcgagtt ttacggggca
    13441 ggtgccgaag caggagccgg gcgacgccac gcacggcaaa gagttgttcg agacgaaagg
    13501 ctgtatggcc tgccattcgc tcggcgaggg cgtggagaat caggtgggcg gcacgttcgc
    13561 cgcgaacctc caacgcgtgg gcgagaaggc cgatttcaat tacatcgtcc gctggattta
    13621 caacccgcgc gagcgttggg cgccctattg cccgaaggaa aaacgcgatc tcacgcgcga
    13681 ggattatgag aagaacggca agccttatcg cttcgacacg gaaggtcatt ccaagtgtcc
    13741 gaacgacggc gcggaactgc aagtgcagaa catgacggtg atgcccaact tccgtctcac
    13801 cgaacaggac tcgcgcgaca tcgccaccta tctcttctcg ctcagcaagg gaactcagtg
    13861 gcctgacgct tctttcatgg acgatccgaa gttgaaggat cagggcgcgt attacatcaa
    13921 gcagtacggc tgcgccaact gccacgagat cagaggcttc gaagacgagc agcgcatcgg
    13981 caaggagttg accgccgaag gggcgacgcc catcgaacgt ctcgacttcg cactgctgac
    14041 gcacgacgcg gagaaaaata tcaacccctt caccgaaaag aagtacgagc gcagggaggg
    14101 gatgcaggag agttggtaca accgcaaagg cttcttcgaa cacaagctcg aagagccggg
    14161 catctacgac aagggcaaag agaaggagcc gcgcgatcac ctgcgcatgc ccgatccgta
    14221 tctcacgccc gaatggaaaa atgcgctgac cacattcctg ctcggcagcg tcggagcgga
    14281 aggggcaaac gtcccgacct ccttcttcta ccagccgact gaccgccgca aggatattca
    14341 agacggctgg tgggtgatca aaaaatacaa ctgcatgggc tgccactcgg tacaggtcgg
    14401 gcaaaagtcg gtgctgcaag atctgccgca atacctgacg cccgaaggca aagaccaact
    14461 cccgcccgcg ttgatgaccg aaggcgcacg cgtcgatccg aactggctgc tgcgcttcct
    14521 gaccgacccg tcgctcagca acggcagcga cgacccgacc gtgcgcggcc tgaccggacg
    14581 caacaacggc ggccagtcag gtagcagagc gagcggcgcg tcaggcggca acggccagac
    14641 gagcggcggt ggtggaagcg gcgcaggggg cggcgaggcg tcgagtaatc aaacgcagag
    14701 cggaggggcg acggcagcat ccggcggcaa cagttccggc ggcaacagtt ccggcgcgtc
    14761 gcagtcgaac gacgctgccc cggaattgcc gccgcaaccg ggcgagaata aaaacggcgt
    14821 gcgtctctac ctgaaagcgc gcatgcccac cttcaacttc tcgccgaacg agttgcgcac
    14881 gctggtacgc ttcttcctcg cagtctcttc ccagcaggag ccgttcatca aagagccggt
    14941 tgacccgctc acggatcagg agagaaatct cgcgcggcag ttgttcactt cgcagcaagc
    15001 gccctgcttg aagtgtcacc tgaccggaaa ccccgagcac gacaaaaacg cttccgctcc
    15061 gaacttcttg caggcgggcg aacgcctcaa gcccggttgg acgttccgct ggctgctcga
    15121 cccgcagaag gtgatccccg gcacggccat gccctccgaa ctcttcaagc gcgacggcga
    15181 acgctgggtc ttcaacggcg aactcccgga gagctttaac gagtacaagg gcgaccacgc
    15241 cgatctgctc gtccgttaca tgctgcaact gacgcccgca gagcaagcgc gcctcgcctc
    15301 cggttcgcct gcggccacgg gcggcaccac cacgacaggc gagagcaaaa acgccgccgc
    15361 gcaatcgagc acgcatcatg cgcgcgcgcg tgccaaggcc gccgcgcgaa acaggaaata
    15421 tcgtttgcgc gcggcggcct cgcgcgctcc gcaaggcagt cggcgcagtg tgagacgtgt
    15481 gcgtgagccg gtcgtgtccg tacggcaata cggcgtcggg tattcgccgc cgcatggacg
    15541 ttcttggtag aatcggggaa attaaatttc aggagacacc tacgaaatgc cattcaagca
    15601 attaagccgt ctgctcgctt tgatgctcgc cgccgcgatg ctctctttca tgctcgcctg
    15661 cggaggcgac gatgatgacg acgtggccga ggaagacggc gcggcgaccg aggcagcggc
    15721 aggcacgcct tacaagtcga gcggcaacga aggctcgatc acgggcgcgg tcaatttcac
    15781 aggcgaaccg cccgcgccga aaccgatcag catggatgcg gacgccgcct gcgcgcagaa
    15841 caacgccaat ccgcagacgg aggatgtcgt cgtcaaagac ggaaaactcg ccaacgtgtt
    15901 cgtctatatc aaggacggca agacgaccga aggcaaaagc atcacgagct ttgccttcga
    15961 agttccggcc accgaagtga cgctcgacca gaaaggctgc cgctacatcc cgcacgtgct
    16021 cggcatcatg gccggacaga agttgaaggt gctgaacagc gacccgaccg cgcacaacgt
    16081 ccacccctcg ccgaagagca acaaggagtg gaaccagtcg caacccgccg gagcgcagcc
    16141 gatcatccag agcttttcgc gccccgaagt ggtcgtcccg gtgaagtgca accagcaccc
    16201 ctggatgaag gccaacatcg gcgtcctgcg ccacccgctc tacgccgtca gcggcgagaa
    16261 cggcgcgttc gagatcaagg gcgtgccgcc gggaacttac acggtcgcca cctggcatga
    16321 gaggtacggc gagaaaactc agtccgtcac cgtcggcgcg aaagagacta agacgcagga
    16381 cttcgctttc gatgcgagcg cgggcaagac cgccatccat ggttcgctcg aagtgctgcc
    16441 cgccatcgag ttcccggtaa tcacgaaaca ttaaaaacag tgacgagtga cgggtgacaa
    16501 gccaaaacag aaaaaccact cttcctgtct tcaacttgtc actcgtcacc ggtcactcgt
    16561 cactaaggat tttgattttg actcgtcact tgtcactgtt cttgtcactg ttcctggcgc
    16621 ttgagaatta ttaaaagggg aagtgcagcc ggttaattta tttcatgtct cgctcggggc
    16681 gagccgcttg ccacccgtcg cacgggatgc gacacttgag tttacttgca gacagaaagt
    16741 taagctttaa gcatcgctcc acaggcattt catggaaaca attgttgtag aagagatcga
    16801 gcaatcggtc gcgcccggcg cggtcgaggg cgggcgttcg gcgacgcgcg agaaattgtc
    16861 cgcatattac gagttgacga aaccgcgcat cacctttctc gtcgtgctca cgacctccgc
    16921 aggcttcctg ctcggttcga cgcgcggcgc gttcgactat caactcttcg cgcacacgct
    16981 cctcggcgtc gcgctgttgt gttccggcat ctccactctg aatcaatgga tggagcgcgg
    17041 cctcgacggt ttgatgcgac gcacggcgcg gcgtccgctt ccgctcggca cgctctcgcc
    17101 gcgcgccgct ctcatcttcg gcttgagtct aaccgtcggc gcggaggctt atctggccgt
    17161 cttcgtcaac ctgctctcgg ccttctgcgg cctgaccgtg atcgtcggct acttgctgtg
    17221 ctacacgccg ctcaagacgc gcacttcgct ctcaactctc gtcggagctt ttcccggcgc
    17281 ggttccgccg cttctcggtt gggcggcggc gacgaatcac gtcggcctcg aagcgtgggc
    17341 gctcttcgcg atcctgttcc tctggcagtt tccgcacttc ttcgccatcg cgtggatgta
    17401 ccgggaagat tatgcgcggg cgggaatttt gatgctgccc gtggtcgagc cggaagggcg
    17461 tctgacgcgc cagcagatcg tcgtctggac gctgatgctc gtgcccgtca gcctggctcc
    17521 ggcggcgctc ggtatctcgg gcgcaatcta tttcttcggc gcgctcgtgc tcggcgcgct
    17581 cttcctgtgg agcagcatcg ccgccgccgt gacgctctca cgccagagcg cgcgccgcgt
    17641 gctcctggct tccgttcttt acttgcccgt gctgtttgtt ctgatggtca tcaatcagtg
    17701 agccataagc agtaagaaaa gtctgttaac tgcccaacgc ttactgctca cagtttactt
    17761 ccgaaggaga agtcatgggt cgtgcgctcg gcgtcttgat ctggttgttg acgtttggct
    17821 ccgtgtggct gttcgtgaac ggtcgctggt ggctgccgcc ttatatctcc gaacatggtc
    17881 cggcttatga tcgccagttc ctaatcacga tcatcgtcgt gggtgtctcg tttgtggcgg
    17941 cgcagatcgg gttgggctgg gcggtctggc gttacggctc gaagggcgac cggacagagc
    18001 gcgccgtcta ttcacacggc aacaatcgtc tggagatcat ctggacggcc atcaccgcgc
    18061 tcatctttat cacgctcggc ttgatggggc agcgcgtgtg ggcgcagttg cacttgaacg
    18121 aagcgcccgc aggctcgacg cgtgtgcatg tcgtcgcgca gcagtttcaa tggaactttc
    18181 attatccggg ggcggacggc acgttcggtc gcaccgaccc gaagtttgtg aaggacgcgg
    18241 aactgaactt cgtcggctta gacgagcaag accagaacgc gaaggatgat caagtcgtcc
    18301 ctgcgctcgc cgtcccggtc ggtcgcccca tcgaactcac gctgcaatcg aaagacgtga
    18361 cgcacgatct cttcgtgccc gaactccgct acaagcagga cgtggtgccc ggcatggcga
    18421 tcaagatgca cttcaccctg acgaaagaag ggcgtttcga actcgcctgt gcggagcttt
    18481 gcggccagct tcattacaag atgaagagtt atttgatcgc gattcccgcc agcgcgtatg
    18541 acgagttggt ggctctgccg caagcgcagt ttcaggcgcg catcacgcag ttgatcaagg
    18601 agtacccgct cggaatcagg caggcgacgc cggctgccgc agcgtcgaga taagcaagcg
    18661 aaccggactc atcccgaagg gagaggagtt ttaaagatgg aagaggccac gaccccgttt
    18721 gagccgcacg ccgccacggc aaccgccgcc ggagcggttg atgatgctgc ggcggctgcc
    18781 agcccgcatg cggcggaggc ggcgcatcac cacggcgcgc cgcagggttt catacgcaaa
    18841 tatattttca gcattgatca taaagtgatc ggcatccagt atctgctgct cgccatcttc
    18901 gcggcgtttg tagggatgtt catgtccgtg ctgatgcgtc tgaacctgac ttggccgggg
    18961 cggaaatgga cgatcctcga aacgctcttt ccgctcggcg cgccgggcgg ggtgatgtcg
    19021 ccggagttct acctctcgct cgtgacgatg cacggcacga tgatggtctt cttcgtcctg
    19081 accaccgcgc cgcagggcgg tttcggcaac tattttctcc cgatccaggt gggcgcggag
    19141 gatatggctt tccccgtcct caacatgctc tcgttctggg tcacgttcgt cggtctggtg
    19201 gtgctcatcg cagccatctt cgtagagggc agcacgatca tcggcaacac ggatttcgtc
    19261 aacaactacc tcggcggcgg cggcacaggc acgctcggac ccatcggcgg gtggacgggc
    19321 tacccgcctc tgagcgcgct cggaaagatc gccggacccg gacagggcgc gggcgcaact
    19381 ctctggatca ccagcatcgc catcttctgc gtcgcgtcgc tgctcggcgc actcaatttc
    19441 atcaccacgc tcatcaacat gcgcgcgccc ggcatgaccc tgatgcgcat gcctctgacg
    19501 tgctggtctt ggtttacgac agcgatcctc gcgctcctcg cgtttccggt gctgctcgcc
    19561 gccggcgtgt tgctgctgct cgaccggcac gcggggacta gtttcttcat tcccggcggg
    19621 ctggtcttta gtgatgcggt gctgccgaac aagggcggct cgccgattct ctggcaacac
    19681 ctcttctggt tcttcggaca cccggaagtc tatatcgcga tcttgccggg catgggcgcg
    19741 acttcgcaca ttctttccac cttcgcgcgc aagcccgtct tcggttatcg cgcgatggtc
    19801 ttcgcgatgt ttgccatcgg cctcctcggc ttcttcgtct gggggcatca catgttcatc
    19861 agcggcatgg acccgcgcac gagcatcgcc ttctccgtcc tgacgctctc catcggcgtg
    19921 ccttccgcga tcaagacttt caactggctc ggcacgctct ggggcgcgaa gatacgtttc
    19981 accgtcccga tgatgttcgc tatcggcttc gtctcg
//
LOCUS       DASWNS010000108         4415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_186765, whole genome shotgun sequence.
ACCESSION   DASWNS010000108 DASWNS010000000
VERSION     DASWNS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186765"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VGB76_03665"
     CDS             <1..759
                     /locus_tag="VGB76_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098033.1"
                     /translation="SLMPEGFLSPFDDLLKAHTNYEFDASCLLAESLLEGVQVSLEGY
                     AFRGQVHTLGILDAEMFPGTISFERFVYPSRLPAPVQARMSQFAQDFIGGIGYDNALF
                     NMELMYNPANGDIHIIEVNPKIASQFPDLFEKVDGTSSYLPLLQVALGVEPTFKRRQG
                     SFKLAASCVLRDFTDRRVLRVPTTEQIAELTRKVPDARIEISVSEGQLLSEIMQDGKS
                     FRYGLINIGANSEAELSAKFELCKSSLQFQFAAP"
     gene            1186..4143
                     /locus_tag="VGB76_03670"
     CDS             1186..4143
                     /locus_tag="VGB76_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098034.1"
                     /translation="MKFAKILACGFYSLFIALLVSFNPLELGAERAAAKSQAASAQDI
                     CAWPLPYLPSQRGEEAFEKLLSDYTNKGCYKNDPRWKHDLEVRDTGAFLSGKTYGTHN
                     AVRVYYSPEIVKWLGSGKEGDRKEGDIPDGAMIIKEMYANPAQQNVDTKIKCNDPFSN
                     QDPQPYRCKGLAIMFRDKRGSWDGWFWSDGNPLNYGSYPNAGFGLYCVNCHASAKTEL
                     TFSTLNNILGNAITFNPTMPPNQIDLRQTTRPRATIAPKQRLTPLEDISVHLKRSEDS
                     EKVKTGADGTMQAPTATAHRSARTVPGASASPTPKQLVSEWYDTATQRPQSQKQQMFL
                     TSNQCIGCHDATQNNATMPNMIYQQIYTNIPASPSPTPSPQELDLNLSPYSEWRSSLM
                     GLSGRDPVFYSQLETELNLYPGIKDQIVNTCLSCHGVMGQRQLTLDTQKPFEFKLEYL
                     DRFGAQPFAKYGALARDGVSCAVCHHISNEGLGTPATYTGKFNLGKADEIYGPYEDVA
                     LVPMKNSLGLTPLKTKENQIGKSALCGSCHTVILPVLKPGQPYAPGDNVFTNPRQETE
                     HEQNTYLEWRNSKYQDERPPINREAAQSCQQCHMPGMYPQKTGEKLQFKVASIEDETF
                     AAVEFRAPDGDIHLMVRGGAASPGENYSRHTLVGLNLFVMEIFNQFSAQLGISTQPDA
                     NNVPIPFYDPMATWGNSVPSMVLTKKSALEMARNETAQIEIASLRRTPKGLTAQVKVT
                     NLAGHKFPSGVSFRRAFIEFRVNDANGNPVWVSGDTNEMGVIGNCRNGQFAAGKCRPG
                     QFVPLLTESFAKSSNPQQIFQPHYETINSNTQVQFYEELVKDKNGNFTTSFLSLANQV
                     KDNRLMPQGWREDGPDAKSTAPGGVDKNSNPGYFDGSGTDVVTYEVPLDPTIRRPLTV
                     TARIYYQTIPPYYLEQRFANAPDGTFTRSLKYYVNNLDTKFIDSRWSLLLNEAPIRDW
                     KLFIVGTPPRTLR"
BASE COUNT         1003 a         1352 c         1176 g          884 t
ORIGIN      
        1 agcctgatgc ccgaaggctt cctgagtccc ttcgacgact tgctcaaggc tcacacgaat
       61 tacgaattcg acgcctcctg cctgctggcc gagtcgctgc tcgaaggcgt gcaggtatcg
      121 ctcgaaggct acgcgttcag agggcaggtt cacacgctcg gcattctgga tgcagagatg
      181 tttcccggta cgatctcatt cgagcgtttc gtctatcctt cgcgcctgcc cgcgccggtg
      241 caagcgcgca tgagccagtt cgcgcaggat ttcatcggcg gcatcggcta cgacaatgcg
      301 ctcttcaaca tggagttgat gtacaaccct gcgaacggcg acatacacat catcgaagtt
      361 aatccgaaga tcgcctcgca gtttccagac ctgttcgaga aggttgacgg gacaagttcc
      421 tacctgccgc tgttgcaggt tgcgctcggc gtagaaccaa ctttcaaacg aaggcaaggc
      481 tctttcaaac tggccgcgag ttgcgtgctg cgcgacttca cagaccgcag agtcctgcgt
      541 gtgccgacca ctgaacagat cgcggaactg acgcgcaaag tccccgacgc gcgcatcgaa
      601 atctccgtct ccgaagggca acttctctcc gagatcatgc aggacggaaa aagttttcgc
      661 tatggcctga tcaatatcgg cgcaaactcg gaagccgagt tgagtgccaa atttgaactc
      721 tgcaaaagct ctctccaatt ccagttcgcc gccccttgaa cgtcgcacag ggcgcacgga
      781 aagccgtctc ccaactttcc gccgtccgca ctttgccgga gtgttcgcgt cgccttcgtc
      841 aactattttc gacgccttgt gagttcgctg tgaacgggcg gctcccgtgt cgcgtgtgcg
      901 cttcgactgt tcggcggcgg ctccccttcg cggataaaag ttgttgacaa gaacgggcgt
      961 ggctcataca gtgcaagagc acttacgtcc ttgcctcgca gctttcaaat tagttcttta
     1021 actctctttt caagttgtcc aacgtattca cactgaaggg tgtagtctcg aagatttaca
     1081 gccccggtgg tgtctaagca tgaccgcgcc gtgtgtgttc aaaaaggcac aggcgcgcgt
     1141 cgtgcttacg cattgagcgt tccctgttgc cttgaggagg caaaaatgaa gtttgcaaaa
     1201 attctcgcct gcggcttcta ctctcttttc atcgccctgt tggtatcctt caatcccctc
     1261 gaacttggcg cggagcgcgc ggctgccaag tcgcaagccg cgagcgcgca ggacatctgc
     1321 gcgtggccgt tgccgtatct ccccagccag cgtggcgaag aggcttttga gaaactcctc
     1381 agcgactaca cgaacaaagg ttgctataag aacgacccgc gctggaaaca tgatctcgaa
     1441 gtgcgcgaca caggagcctt cctctccggc aagacctacg gcacgcataa cgcggtgaga
     1501 gtctattatt cgcctgagat cgtgaaatgg ctcggcagcg gcaaagaagg cgaccgcaaa
     1561 gaaggcgaca tccccgacgg cgcgatgatc atcaaggaga tgtatgccaa tcccgcgcag
     1621 caaaacgtcg acacgaagat caaatgtaac gatccgttca gcaaccaaga cccgcagcct
     1681 tacaggtgca aaggactcgc catcatgttc agggacaagc gaggctcttg ggacggctgg
     1741 ttttggagcg acggcaaccc gctcaattat ggcagctacc cgaatgcggg cttcggtctg
     1801 tattgcgtca attgccatgc ctccgcaaag accgaactga ccttctccac cctgaacaac
     1861 attctcggca acgccatcac tttcaacccg acgatgccgc ccaatcagat cgatctccgg
     1921 cagacgacgc gcccgcgtgc gacgattgcg ccgaagcaac ggctcacgcc gcttgaagat
     1981 atttccgtcc atttgaagag gtctgaagac agcgagaaag ttaagaccgg cgcggacggg
     2041 acgatgcagg ctccgacggc gacggctcat cgttccgcgc gcaccgtccc gggggcgtcc
     2101 gcctcgccca cgccgaaaca gttggtcagc gagtggtacg atacggccac acagcgtccg
     2161 cagtcgcaaa agcagcagat gttcctcacc tcgaatcaat gtatcggctg ccacgatgcg
     2221 acgcagaaca atgccaccat gcccaacatg atctaccagc aaatctatac caacatccct
     2281 gccagccctt cgcccacgcc ctcgccgcag gagttagacc tcaatctctc tccctacagt
     2341 gaatggcgtt cttcgctgat gggtctatcc gggcgcgatc cggtcttcta ctcgcagttg
     2401 gagacggagt tgaatctcta tcccggcatc aaagaccaga tcgttaacac ctgcctgagt
     2461 tgccacggcg tgatgggaca acggcaactc acgctcgaca cgcagaagcc cttcgaattc
     2521 aagttggagt atctcgaccg tttcggggcg cagcctttcg ccaagtacgg cgcactcgcc
     2581 cgcgacggcg tatcgtgcgc cgtctgccat cacatctcca acgaagggtt gggcacgccc
     2641 gcaacctata cgggtaagtt caacctgggg aaagccgacg agatttatgg tccttatgag
     2701 gatgtcgcgc tcgtcccgat gaaaaattct ctgggcttga cgccgctcaa gacaaaagag
     2761 aatcagatcg gcaagtccgc gctctgcggc tcttgtcaca cggtgatcct gcccgtgctc
     2821 aagccggggc aaccttacgc gcccggcgac aacgtcttca ccaacccccg gcaggagacg
     2881 gagcacgaac agaacaccta tctcgaatgg cgcaacagca agtatcagga cgagaggccg
     2941 cccatcaatc gagaggcggc gcaatcctgc cagcaatgcc acatgccggg gatgtacccg
     3001 caaaagaccg gcgagaagtt gcaattcaaa gttgccagca tcgaagacga gacgtttgcg
     3061 gctgtggagt ttcgcgcgcc ggatggagac atacacttga tggtgcgcgg cggcgcggcc
     3121 agtcccggcg aaaattattc acgccacacg ctcgtcggac tcaacctgtt cgtcatggag
     3181 atcttcaacc agttctccgc gcaactcggt atttcgacgc agcccgatgc aaacaacgtc
     3241 ccgattccct tctacgatcc gatggcgacg tggggcaact ccgtccccag catggtgctc
     3301 accaagaaat ccgcgctcga aatggcgcgc aacgaaaccg cgcaaatcga gatcgcttcg
     3361 ctgcgccgga cgccgaaagg tctgacggca caagtcaaag ttaccaatct cgccgggcac
     3421 aaattccctt cgggcgtgag tttccggcgg gcgttcatag agtttcgcgt caacgacgcg
     3481 aatggcaatc ccgtctgggt ttcgggcgac acgaatgaga tgggcgtgat cggcaattgc
     3541 aggaacgggc agtttgcggc gggcaagtgc aggccgggac agtttgtgcc cttgctgacc
     3601 gagtcgttcg ccaagtcgtc caacccgcag caaatctttc agccgcatta cgaaaccatc
     3661 aacagcaaca cacaggtgca gttttatgaa gaactcgtga aggacaagaa cggcaacttc
     3721 acgaccagtt ttctgagtct cgccaatcag gtcaaagaca atcgcctcat gccgcagggc
     3781 tggcgcgaag acggccccga tgcgaaatcc accgcgccgg gcggcgtgga caagaactcg
     3841 aatcccggct acttcgacgg cagtggaacc gacgtggtga cctacgaagt gccgctcgac
     3901 ccgaccatca ggcgtccact caccgtcacc gccagaatct actatcagac catcccgccc
     3961 tactacctcg aacaacgctt cgccaacgcg ccggacggta ctttcaccag atcgctcaaa
     4021 tactacgtca acaacctcga cacgaaattt atcgactccc gatggagctt actgctcaac
     4081 gaagcgccca tccgggattg gaaactgttc attgtcggca cgccgccccg gacgcttcga
     4141 taaactccgg gcagccccgc agaagacaca aaaccctgcg cccgtaacgc gtgtttaaaa
     4201 cagtcggcct gattaagaga gaacagtttc gtcaggccga ctgtttttcg cgtcgctcgt
     4261 ctctctatcg cgtcgctcgt ctctctatcg cgctgctcgt ttgattatga attgaggttt
     4321 cgcaggaggc cgtcgaaata tgcggcgatc tttttcccga acacttctcc gcgttcgata
     4381 tatcgcgcaa agattgaagg gttgatgagt tccgc
//
LOCUS       DASWNS010000109         5335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_472552, whole genome shotgun sequence.
ACCESSION   DASWNS010000109 DASWNS010000000
VERSION     DASWNS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472552"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..476
                     /gene="rpsT"
                     /locus_tag="VGB76_03675"
     CDS             210..476
                     /gene="rpsT"
                     /locus_tag="VGB76_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY0098035.1"
                     /translation="MPNHKSAEKRMRQNERRKGINRSNRTRLRTEIKKLRAALSGGDK
                     DALQAALSQTVSTIDKSVQKGVLHKNAAARYKSRLTARVNQAGA"
     gene            585..1025
                     /locus_tag="VGB76_03680"
     CDS             585..1025
                     /locus_tag="VGB76_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098036.1"
                     /translation="MVAGEQAKQRFRERREIERLVEGFEACTVAPVEFDHGAHLAVAL
                     WYLSELPAPLAEERMRAGLHRYTAHHNAQAMYNETLTLFWLKLVRHFLARADSKRPLA
                     ERGNELLTTYRSSKLVFEYYSRELILMPEAKTSWIEPDLKPLDF"
     gene            complement(1078..2082)
                     /gene="holA"
                     /locus_tag="VGB76_03685"
     CDS             complement(1078..2082)
                     /gene="holA"
                     /locus_tag="VGB76_03685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY0098037.1"
                     /translation="MSLLTREKLWSELKAKRVAPLYLLFGAEDYLRDAAARRIADEAL
                     SGASLREFNEATYSLASVDVQQAIAAAEQLPMMAARRVVRIADFAKLREADEGALLRY
                     IARPAESSVVIFIADELDKRRKLSKTLLDACVSVEFATLADNELAVWARDQLKRLKAD
                     ADERTLRQIIALVGTSVRRLANELDKLATAALTLDGRITMELVETLVNHSREHSNFEL
                     TDRLIAKDRKGALKTLRRLLDDDAEPVMLVGLIASNYHRLALTKELMSAGKPKEEVFR
                     LVPMPYGKREEFLATARRTDSADLARRISRIASTDLAIKTSLGTPRLQLELLICELSS
                     "
     gene            complement(2079..2642)
                     /locus_tag="VGB76_03690"
     CDS             complement(2079..2642)
                     /locus_tag="VGB76_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HEY0098038.1"
                     /translation="MTLAKKLVRGLSLLALLACVSGFTECYKPVTKSGLPTHIRTVAV
                     PPFQNQALRYKIEARFTEAVSNEIIRRGHGLRVQSDPEGADAVVDGVIRNFAFGGVLL
                     DERGRARIFEVTVVGAVTVRDQVQNRVLYDNQNYVYRGEFEFTNDPRTFFNEEDPAVQ
                     RIARNFAESLVSTLVHGFGVNEEKKKK"
     gene            complement(2835..3710)
                     /gene="folP"
                     /locus_tag="VGB76_03695"
     CDS             complement(2835..3710)
                     /gene="folP"
                     /locus_tag="VGB76_03695"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY0098039.1"
                     /translation="MESRSTHEWRLARRTLALGGRTLVMGILNVTPDSFSDGGQFFDL
                     ERAVAHAGEMLTDGADIIDVGGESTRPGGALVSAEEELRRIVPVVERLGAQLPGALIS
                     VDTTKAEVARAALDAGAEIVNDISALRFDPSLADEAARSGAGLVLMHSRGTPETLHKL
                     PPVHAILPEVTESLRRSIALAERRGVARASIALDPGIGFGKSQGQNLELLARLDELAA
                     EFAEFPLLVGTSRKSFIGRLLDDAPVERRLHGTMASVTAAILGGAHVIRVHDVRAASE
                     TARIADAIRAVTLEQ"
     gene            complement(3870..>5335)
                     /gene="ftsH"
                     /locus_tag="VGB76_03700"
     CDS             complement(3870..>5335)
                     /gene="ftsH"
                     /locus_tag="VGB76_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY0098040.1"
                     /translation="KRVSFNDVAGVEEAKEELQEIIEFLKEPQKFQKLGGRIPKGVLM
                     MGPPGTGKTLLARAVAGEANVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCII
                     FIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNDGVILMASTNRPDVLDPA
                     LLRPGRFDRRVVVSRPDVRGREGILKVHTRKIPLGEDVDISILARGTPGFTGADLANL
                     VNEAALNAARYNKKIVMQNDFELAKDKVLMGAERKSMVISNEEKRITAYHEAGHTLVG
                     LKVPNADPVHKVTIIPRGMALGLTQQLPEGDRYSATREYLLGQIAIMMGGRLAEEIFL
                     NDITTGASNDIERATELARAMVCEYGMSDLGPLTFGKKEEQIFLGREISQHRDFSEDT
                     AIKIDQEVRKIVNEQYERARNIITENRETMIRLSETLLERESLDGVQIRRIVAGLPLD
                     EEPPPASKDDEERPQLKEPATPPLKPILPPITGPATA"
BASE COUNT         1005 a         1634 c         1613 g         1083 t
ORIGIN      
        1 gcatgttgca ggtaaaacgc tactacatgc cccccgccgt gtcaacgaag acggcaaccg
       61 ccgacccgcg ccagcaccaa cctcgccttg acaagaactc tccgtgatac ctatactttg
      121 ccctttgcgt tcaaggccaa atcgttggct gtagcggcgc aagttgaatc aaacaatagt
      181 ttttagattt actgacggag tttcggaaca tgcctaatca taaatcagcc gagaaacgta
      241 tgcgccagaa cgagcgccgc aaaggaatca atcgcagcaa ccgcacgcgc ctgcgcacgg
      301 agatcaagaa actgcgcgcc gcgctctccg gcggcgacaa ggacgcgctg caagccgcgc
      361 tttcgcagac ggtttcgacc atcgacaagt cggtgcaaaa gggcgtcctg cacaagaacg
      421 ctgcggcgcg ctacaagtcg cgtctgacgg cgcgcgtcaa tcaagcgggc gcgtaagtta
      481 aacgttcctc gaacaattca agcggcagcg aggcggatcg aacagttcgg tccgcttcgc
      541 tgttttttct ttggacttaa agagcggcaa aacggaggtc agacatggtg gccggagaac
      601 aggcgaagca gcgtttcaga gagaggcggg agatcgagcg tttggtagag ggttttgagg
      661 cttgcaccgt cgcccccgta gagtttgatc atggcgcgca tctggccgtc gcgctgtggt
      721 atctctcgga gttgcccgcg ccgctcgcgg aagagcgcat gcgcgcgggc ttgcatcgct
      781 acacggcgca tcacaacgcg caggccatgt acaacgaaac tctcacgctc ttctggctca
      841 aactcgtccg ccactttctc gcacgcgcgg actcaaaacg ccctctcgcg gagcgcggca
      901 atgaattgct cacgacttac aggagttcaa agctcgtctt cgaatattac agccgcgagt
      961 tgattctgat gccggaagct aaaacttcgt ggatcgaacc cgacttgaag ccgctggatt
     1021 tttgaggcga agaatgggag cgggatcgca caggtttttg tttgcggcgg gtgagcgtta
     1081 gctcgatagt tcgcagatga gcagttcgag ttggaggcgg ggggtgccga gagaagtctt
     1141 gatggcgagg tcggtggagg cgatgcgcga gatacggcgc gcgaggtctg ccgagtcggt
     1201 gcggcgcgcg gtggcgagaa actcttcgcg cttgccgtag ggcatcggca cgagccggaa
     1261 gacctcttct ttgggcttgc ccgccgacat caactctttg gtgagcgcga ggcgatggta
     1321 gttgctcgcg atcaacccga cgagcatcac cggttccgca tcgtcgtcga ggagccgccg
     1381 cagagtcttc agcgcgccct tgcggtcttt ggcgatcagg cgatccgtca actcgaaatt
     1441 cgaatgctcg cgcgaatggt tgacgagagt ttccaccagt tccatcgtga tgcgcccgtc
     1501 gagtgtgagc gcggcggtcg ccagtttgtc caactcgttc gccagccgcc gcacgctcgt
     1561 tcccaccagc gcgatgatct gccggagcgt acgctcgtca gcatccgcct tcaacctctt
     1621 caattgatcg cgcgcccaca cggccagttc gttatccgca agcgtcgcga attcgacgct
     1681 cacgcaggcg tccagcagcg tcttcgacaa cttgcgccgc ttatcgagtt cgtccgcaat
     1741 gaagatgacg accgaacttt cagcggggcg cgcgatgtag cgcaacagcg cgccctcgtc
     1801 cgcctcgcgc agcttcgcaa agtctgcgat gcgcacaacg cggcgcgcgg ccatcatcgg
     1861 gagttgctcg gcggcggcaa tcgcctgctg cacgtccacg ctcgcgaggc tataggtcgc
     1921 ttcgttgaac tcgcgcagag atgcgccgct cagagcctcg tcggcgatgc gacgggctgc
     1981 ggcgtcgcgt aagtagtctt ccgcgccgaa cagaagatag agcggcgcaa cgcgcttcgc
     2041 cttcaattcg ctccacaact tttcgcgcgt gagcaggctc atttcttctt cttctcttcg
     2101 ttgactccga agccgtggac gagtgtggag acgagacttt cggcgaaatt gcgcgcgatg
     2161 cgctgcaccg cagggtcttc ttcgttgaag aaggtgcgcg ggtcgttggt aaattcaaac
     2221 tcgccccggt agacgtagtt ttgattatcg tagagcacgc ggttctgcac ctgatcgcgc
     2281 accgtcaccg cgccgaccac cgtgacttcg aagatgcgtg cgcgcccgcg ttcgtcgagc
     2341 agcacgccgc cgaaagcgaa gtttctgatc acgccgtcaa ccaccgcgtc cgcgccttcc
     2401 ggatcgctct gcacgcgcag gccgtggccg cgacgaatga tctcgttcga gacggcttcc
     2461 gtaaaacgcg cctcgatctt gtagcgcaac gcctgatttt gaaaaggcgg gacggccacc
     2521 gtgcgaatgt gcgtcggcaa cccgctcttc gtcacaggct tgtagcactc cgtaaagccc
     2581 gacacacagg ccagcaaggc cagcaacgac aacccgcgca caagtttttt cgccaaagtc
     2641 attttcaagt ctgctcccct cgcaccgaac gcgaacgccg cacgatacga taaactggag
     2701 aacagtgaca agtgacgggt gacgagtgac aagttaaaac agtagatcgt gaatagagaa
     2761 acaggtctgc ttctattcac acttgacgat tccgggttca cagctttcac tcgtcactcg
     2821 tcacccgtcg cttgtcactg ttctaaggtt acggctctta tcgcatccgc gatgcgtgcg
     2881 gtttcgctgg cggcgcgcac gtcgtggacg cgtatgacgt gcgcgccgcc gaggatcgcc
     2941 gccgtgacag aggccatcgt gccgtgcaga cgcctctcca cgggcgcatc gtcgagcaga
     3001 cgcccgatga aagacttgcg cgacgtgccg acgagcaatg gaaactctgc gaactccgct
     3061 gccagttcgt cgagccgtgc gaggagttcg aggttctgtc cctgactctt gccgaagccg
     3121 atgccggggt cgagcgcgat gctcgcgcgt gccacgccgc gccgttcggc gagagcgatg
     3181 ctgcggcgca agctctctgt aacttccggc aggatagcgt ggacgggcgg caatttgtgc
     3241 agcgtctcgg gcgtgccgcg cgagtgcatg agcacgagtc cagccccgct gcgcgcggcc
     3301 tcgtcggcga gcgaagggtc gaaacgaagc gcggagatgt cgttgacgat ctccgctccc
     3361 gcgtcgagcg cggcgcgcgc cacttccgct ttcgtcgtat cgacagagat gagcgcaccg
     3421 ggaagttgcg cgccgaggcg ttcgacgacg ggcacgatgc ggcgcaactc ttcctccgcc
     3481 gaaaccagcg cgccgcccgg cctcgtcgat tcaccgccga catcaatgat gtccgcgccg
     3541 tctgtgagca tctctccggc gtgcgcgacg gcgcgttcga gatcaaagaa ctgtccgccg
     3601 tcgctgaagc tatcgggcgt cacgttgagg atgcccatga cgagcgtgcg cccgccgagc
     3661 gcgagcgtgc ggcgtgccag tctccattcg tgtgtgctcc tactctccat cctctggaaa
     3721 cataattaac cacaaagagg cgcaggcaca aaggaagcga taaacgtttt ctttgcgccc
     3781 gcgcctcttt gtggttaacg actcaaagcg acaaaaaggg gaagaggttt gatccccttc
     3841 ccctttcgct gtctgcttct acctttaact tacgccgtgg ccgggccggt aatgggcggc
     3901 aggatcggct tgagcggcgg cgtcgcaggc tctttcagtt gcggacgctc ctcgtcgtct
     3961 ttcgacgccg gaggcggttc ctcgtcgagc gggagtcccg cgacgatgcg gcgaatctgc
     4021 acgccgtcga gcgattcgcg ttcgagcaac gtctccgaga gccggatcat ggtttcgcgg
     4081 ttctctgtga tgatgttgcg cgcgcgctcg tattgctcgt tgacgatctt tctgacttcc
     4141 tgatcgatct tgatcgcggt gtcttccgag aagtcgcggt gttgcgagat ttcacgaccg
     4201 aggaaaatct gctcctcttt cttgccgaag gtgagaggtc cgaggtcgga catgccgtac
     4261 tcgcagacca tcgcgcgggc aagttccgtc gcgcgttcga tgtcgttcga cgcgccggtc
     4321 gtgatgtcgt tcaggaaaat ctcttccgcc agccgtccgc ccatcatgat cgcgatctgg
     4381 ccgagtagat actcacgggt cgcgctgtag cggtcgcctt ccggcaactg ctgcgtgagg
     4441 ccgagagcca tgccgcgcgg aatgatcgtg accttgtgca cggggtctgc gttcggcact
     4501 ttgaggccga cgagcgtgtg ccccgcctcg tgataggccg tgatgcgctt ctcctcgttg
     4561 gagatgacca tgctcttacg ctccgcgccc atcaggactt tatccttcgc caactcgaag
     4621 tcgttctgca tgacgatctt cttgttgtag cgcgcggcgt tcagagcggc ctcgttgacg
     4681 aggttggcga ggtctgcgcc ggtaaagccg ggtgtgccgc gtgcgaggat cgaaatatcc
     4741 acatcttcgc cgagcggaat cttgcgcgtg tgaactttga ggatgccttc gcgtccgcgc
     4801 acgtcggggc gcgagacgac cacacgacgg tcgaagcgtc cgggtcggag caacgcggga
     4861 tcgagcacgt ccggcctgtt ggtcgaagcc atcaggatca cgccgtcgtt cgattcgaag
     4921 ccgtccatct cgacgagcaa ttgattcaaa gtctgctcgc gttcgtcgtg gccgccgccg
     4981 aggcctgcgc cgcgatgacg cccgaccgcg tcaatctcgt caatgaagat gatgcacggc
     5041 gcgttcttct tgccctgctc aaacaggtcg cgcacacgcg atgcgccgac gccaacgaac
     5101 atctccacga agtcggaacc ggagatcgag aagaaaggca cgttcgcttc gcccgcgacg
     5161 gcgcgtgcca gaagcgtctt gcccgttccc ggaggtccca tcatcaaaac gcccttcggg
     5221 atgcgcccgc cgagtttctg aaacttctgc ggctctttga ggaattcgat gatctcctgc
     5281 aattcctctt tcgcctcttc gacgcccgcc acgtcgttga acgagactcg cttct
//
LOCUS       DASWNS010000110        11368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_367163, whole genome shotgun sequence.
ACCESSION   DASWNS010000110 DASWNS010000000
VERSION     DASWNS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11368
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367163"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(443..5113)
                     /locus_tag="VGB76_03705"
     CDS             complement(443..5113)
                     /locus_tag="VGB76_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0098041.1"
                     /translation="MKLSKVLLLTLCLLALVPQLALTQESDAVGRLRRRAGAIPNNYI
                     VVFKDSVPQGRVKAFAAQLARTHGGTIGFTYQHALRGFSVDLSEAQALALSRNPQVEY
                     VEEDTLIEGVGVQSDPTWALDRIDQRLLPLNAAYNYANSGAGVNAYIIDSGIRFKHQE
                     FGERVLFAYDNVADGRNGEDCIGHGTHVAGIIGGKTYGVAKDAKLWAVRVINCQNQGS
                     SARNIAGIDWVIANHVKPAVANLSFITIVGNDSIDLAVRNLIAAGVTTVVAAGNNTGD
                     ASLRSPARVMEAITVAATDQFDHQSPNSNFGSVVDLYAPGVDIVSAWHFDNASYQPRS
                     GTSFAAPFVAGLAARYLASRPGDQPDAVGQAIINAATPGKVINPGAGSPNLLAYAGIT
                     LSDDFNDNVRDPTKWHAPAAPDFTMVETNGRLEITPGANATSYDGYKSATTVDLTDAR
                     ASIEVSAAHRIDSFGIYFTLGHSTGNNLMFGLGGSHFVLQQEVGGFTTTTHLAYDAVQ
                     HRFWRFRHNRANDTVNWEVSPDGTTWTVLRSEPRPFSITDLQIRLIAGKWSATTPTTT
                     ATFDNLWHEPNPTPPLVMADNFDDNLINPAMWATPDQTSPTIVSELNGRIEVMLQPNI
                     AAYNALHLARTFDFRDKTLQVEVQPASQAGGVETSFQLYFDNSNYFIFGAGAGSLTCD
                     ALVNGVRDRTSLDWDDSRFWRFRHDADANTVNFETSMDGTTWATRKTVTPAFPLNTLR
                     AALAAGASGTGNSAPGAAIFDNFRLERYRPLFPQSDNFNDNVRDARKWNAPTLPDFVI
                     AEQNGRLEIRPGTTSANYDGYQAATTFDLTDARASVQASTVQRIDGLGSYFVLRHSVG
                     NDLVFGIRGSNLVMQQEVGGVITAPTLPYDAVQHRFWRFRHNRANDTMNWETSPDGAT
                     WTTQSTMPTPFPITNLQTLLIAGKQTAAIPTGTALFDNLRIERNEGGLARTGSNTVND
                     APPANAAPQVNAGSDATVNEGGTFSGTASFTDIDNTDTWMVTVDYGDGTGAQPVAPTP
                     SKTIPLSHVYKDNAANPYMVTVTVTDAAGASSTDTAVVMSVNAPPTPVITGAQSTIRE
                     ETGLTLGSTVSDPGEADTAAGFKYRWTVTKYGVAYTPREVSSQSTFNFWEWAGTYVVT
                     LHVMDKDGGAGTTSTTITVNKIEQNDNFDDNSMDTLRWYVKGAPYVTVAEQNGQLQVT
                     THLSTLGYDGYYGRRSFDLTTHSAAVEAVKPAYNAGLDTYFALEDVMITGNRLLFAVR
                     GYMFEGPGSLLMEEHVNDTVTSRTQIPYDAAQHRFWRFRRDPATDTLNWETSPDSTHW
                     TVRHTAPRRPSLTRVRAQLYAGRRTSSGGSPRTAVFDNYRVEDVPQPPTISFSDNFDD
                     NLLDSALWTVDDPASSSSVSEQNQRLEVLLASAYIWDTGNSIKSASFYNFWNRTLQVE
                     VPRVSQVQGSTTYFELHSHSVNYSMSVTSNMLNCDVMIDTTHRTSVAYSASAHRFWRF
                     RHDAAAHTINFEVSADGHAWTTLKTVPETEPLDSMRVVIGARNEGSTSDTSVFDNVKM
                     EANQ"
     gene            complement(5651..7000)
                     /locus_tag="VGB76_03710"
     CDS             complement(5651..7000)
                     /locus_tag="VGB76_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0098042.1"
                     /translation="MKKNLQYLRQPLLLCVIWASCFVACPAQDVGAQVDEYLKSAITR
                     SRFSGAILIAREGRIIVRAGYGMANVEHDVPVTPETKFRIGSLTKQFTAAAILILQQR
                     GKLQTQDAICKYLPGCPAVWQPITIHQLLTHTAGLPNVDHTVEAKAPMSLAYTVQTFK
                     EQPLEFKPGAQFRYSNTNYLLLGYIIESISGVPYDRFLDENIFQPLKMIDTGYDNHER
                     LLKQRAAGYSLRATQLVNASYVDMSVPFSAGGLYSTVDDLYRWEQSLSTETLLSKRSV
                     DAIFTPYKGGYGYGWYIGEQDGRTCLSHGGWIGGFASALARYPNDKLTVIVLSNFDNL
                     PVNTMARHLAGIFFGAKGATLKDQKVASINPQILDGYVGQYELTPTFLITITKEGDKL
                     MGQAAGFPRIELLPASDIEFFVKEFDAQILFVEDGKGTVTHSILTLNGREAQAKKVH"
     gene            complement(7179..8120)
                     /locus_tag="VGB76_03715"
     CDS             complement(7179..8120)
                     /locus_tag="VGB76_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098043.1"
                     /translation="MKNSLRRLTNFVLAAAIFALTIPIAPVSATVQDKGKDKSAKKME
                     SFRGEAAKQHVRALRARHKGLNRAMKDMEKMGKQVDWDQTAVLFTIDPSISQKDRDAR
                     AKIQNVSFSAQDSYQSNADGEMSFITYSGSGSYWDGTVYVTEYATGATDVYNSVVDSG
                     GPVEFTESAYEFDEVYYPPDGGEPIGGGGGGGCGGDGGMRTYDQPQMLCYETMYMTDG
                     KTKSGNEAVFQKASFKRVMAYGFFSWLAKFFKCLGKATSHFAERCVAPSPNFRNNLIC
                     AVTAAFQAAGCCAANAKRSRGPLNPNNTGPGGGYCYR"
     gene            complement(8837..9553)
                     /locus_tag="VGB76_03720"
     CDS             complement(8837..9553)
                     /locus_tag="VGB76_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0098044.1"
                     /translation="MQYSNKPPLAFVNKLLASLPREEYARLNRHLEPASLKKGKIIYY
                     AGNPVREAYFPLGGMISLVSTTEEGYGVEVAMIGNEGMAGVSAILRGISAPYQVMVQI
                     PGNAMRIKSELLIREFARGEQLNNLLLRYTHTLVSQISQSAVCNRFHTAEARLCRWLL
                     TSHDRVGADTLNLTQEFLAYMLGSPRTSVTLIAGKLQGQKLIRYSRGKIKILDRAGLE
                     RGSCECYRVVSHDISHFLAA"
     gene            9732..10100
                     /locus_tag="VGB76_03725"
     CDS             9732..10100
                     /locus_tag="VGB76_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098045.1"
                     /translation="MGKRLRNLRFGTEMSASGACNSIAAMTRPPLFKRRRERTGDFFA
                     RCIVRYPEGVHWVAKVLVEYRQVHGAGAEGLCATTVREPERNHAGWQYVIEKWRRYPT
                     FAATAERLAAKHLVLCNVSS"
     gene            complement(10222..10902)
                     /locus_tag="VGB76_03730"
     CDS             complement(10222..10902)
                     /locus_tag="VGB76_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098046.1"
                     /translation="MRNLTQRTTASRFSLNLILATLLILPAGFVTQTTAQEPEDARRA
                     RISEALSRDDHVTVEKLLAEALAEQNSAEAVAPGPINGSLAYEEIRACGFYPQETRLE
                     CVIDIKQNFGYGGPLETFGSTEYVSFCVDWNNDGVFQNTESVGQGNVVLHDGSGKTNY
                     AVYRDINIPGGLRTRTTAPSTATTITNGPILRARATLSWVLPPTGCNFTPVWGNVFNF
                     RIRLDPIR"
BASE COUNT         2295 a         3077 c         3443 g         2553 t
ORIGIN      
        1 gcgacggcat gcctgacatt gcaggcgttg cgagccggtg cggttaaagc ctgatttcac
       61 ttggcgctcg ctactgtggc aatgaggaca ttgatgcatg tcgagtctta tatcatcatc
      121 aggtctaatt taggcaatcc cgtaacaaat agtctgttga cgagcgtgaa agtccacgcc
      181 acaataggtt gtcataggca cctcctttgg cgatctcatg cgcaacacga gtttatcgca
      241 gctcatacct gagttggagg tgtctgcttc ataatatcac taccgagtaa gagtgcttgt
      301 ccgagttctc actgccttct aacggggcaa gccgtctagg ttaatacagt cgtgtctcag
      361 ttactcgggc ggctgcccct cgccgacagc cggacagaac atccggcggg gagacgcggc
      421 cgcccgagcg ttgaggttcg ctttactggt tcgcctccat tttgacgttg tcgaagacgg
      481 aggtgtcact cgtgctgccc tcattgcgag cccctattac cactctcatc gagtctagcg
      541 gttccgtctc cgggacggtc ttgagggtcg tccacgcgtg accgtcggcg ctgacctcga
      601 agtttatcgt gtgcgccgcg gcgtcgtggc ggaaccgcca gaagcgatgg gccgacgcgc
      661 tataggcgac gctcgtccgg tgtgtcgtgt caatcatgac atcacagttg agcatgttag
      721 aggtgacgct catgctgtag ttaacagagt gactatgtaa ctcgaagtag gtggttgacc
      781 cctgtacctg gctcaccctc gggacttcga cctgcagcgt cctgttccag aagttgtaaa
      841 acgaggccga cttgatgctg ttgccggtat cccagatata ggccgatgct aataacacct
      901 ccaaccgctg gttttgctcg ctcaccgagg atgacgacgc agggtcatcc accgtccaga
      961 gcgccgagtc gagcaggttg tcatcgaagt tgtcgctgaa ggagatcgtt ggcggctgcg
     1021 gaacgtcttc gacgcggtag ttatcgaaga cggccgtcct aggcgacccc ccgcttgacg
     1081 ttctcctgcc ggcataaagt tgggcgcgca cacgtgtgag gctgggcctg cgcggcgcgg
     1141 tgtggcgcac cgtccagtgg gtgctgtcgg ggcttgtctc ccagttgagg gtgtcggtcg
     1201 ccgggtcgcg gcggaagcgc cagaagcgat gctgcgccgc gtcgtagggg atttgcgtgc
     1261 gcgaggtgac ggtgtcattg acgtgctcct ccatgagtaa gctgccggga ccctcgaaca
     1321 tatacccgcg gacggcgaac aggagacggt tccccgtgat catgacatcc tcaagggcga
     1381 agtaagtatc cagcccggca ttgtaggcag gcttaaccgc ctccaccgcg gcgctgtggg
     1441 tcgtcaggtc gaagctcctg cgtccgtagt agccgtcgta gccgagggtg gagagatgcg
     1501 tcgtcacctg aagctgcccg ttctgctccg cgacggtcac gtagggcgct cccttcacat
     1561 accatcttag tgtgtccata gagttgtcgt cgaagttgtc gttctgctct attttgttta
     1621 cggtgatggt cgtactggtc gtgcccgcgc cgccgtcctt gtccatcacg tgcagcgtca
     1681 cgacgtacgt gccggcccac tcccagaaat tgaaggtgct ctgactgctc acctcccgcg
     1741 gggtgtaagc gacgccgtac ttcgttaccg tccaacggta cttgaagcct gccgccgtgt
     1801 cggcctcgcc cgggtcgctt acggtgctgc cgagcgtgag acccgtctct tcgcggatgg
     1861 tggactgagc gcccgtgatg acgggcgtcg gcggagcgtt gacgctcatc acgacggcag
     1921 tgtcggttga ggaggcgccc gcggcgtccg tcaccgtcac cgttaccatg taggggttgg
     1981 cggcgttgtc cttgtagacg tggctgagcg ggatggtctt cgacggggtc ggggcgacgg
     2041 gctgcgcgcc cgtcccgtcg ccgtaatcga cggtgaccat ccacgtgtcc gtgttgtcaa
     2101 tgtcggtgaa ggaggcggtg ccgctgaagg tgccgccttc gttgacagtc gcgtcggaac
     2161 cggcgttgac ctgcggcgcg gcgttggccg gcggcgcgtc attgaccgtg ttgctgcctg
     2221 tgcgcgccag tccgccctcg tttctttcga tccgaaggtt gtcgaacagg gctgttccgg
     2281 tcgggattgc cgctgtctgc ttcccggcaa taagtagggt ttgcaggttc gtaatgggga
     2341 acggagtcgg catagtgctc tgtgttgtcc aggttgcgcc gtcggggctg gtttcccaat
     2401 tcatggtgtc gttcgcgcgg ttgtggcgga aacgccagaa gcgatgctgc accgcgtcgt
     2461 agggaagtgt cggagccgtt atgacgccgc ccacctcctg ctgcatcacg agattgctgc
     2521 cacggatgcc gaacaccaga tcgttgccga ccgagtgacg cagcacaaag taagagccta
     2581 atccgtcgat acgctggacg gtggaagctt ggacggaggc gcgagcatcg gtcaggtcaa
     2641 aggttgtggc cgcctggtag ccatcgtagt tggccgaggt tgtgcccggc ctgatctcta
     2701 gtcgcccgtt ctgttcggcg atgacaaagt cgggcaacgt gggcgcgttc cacttcctag
     2761 cgtcgcgcac gttgtcgttg aagttgtcgc tctgcgggaa gagcgggcgg tagcgttcga
     2821 gtcggaagtt gtcgaagatg gccgcgccag gtgcgctgtt gcctgtgccc gatgcaccgg
     2881 cggcgagcgc ggctctcaac gtgttgagcg ggaaggcggg ggtgacggtc ttgcgcgtcg
     2941 cccacgtagt gccatccatg ctcgtctcga aattgacggt gttggcgtcg gcgtcatggc
     3001 ggaaacgcca gaagcggctg tcgtcccagt cgagcgaagt gcggtcgcgc acgccgttca
     3061 ccaaagcatc gcaggtgaga gagccagcac ccgcgccgaa gatgaaatag ttgctgttgt
     3121 cgaaatacaa ctggaaagag gtttcgactc ctcccgcctg actcgccggt tgcacttcga
     3181 cctgtaacgt cttgtccctg aagtcgaagg ttcgcgccag atgcagagcg ttataggcag
     3241 cgatgttcgg ctgaagcatc acttcgattc gcccgtttag ttcgctgaca atggttggcg
     3301 aagtctggtc gggagtcgcc cacatcgctg ggttgatcag gttgtcgtcg aagttgtcgg
     3361 ccatcacgag gggcggcgtc gggttcggct cgtgccagag gttgtcgaaa gtcgccgtgg
     3421 tcgtcggcgt cgtcgccgac cacttccctg cgatgagcct gatttgcaag tcggtgatcg
     3481 agaacgggcg cggctcggag cgcaacaccg tccacgtcgt gccgtccggg ctgacttccc
     3541 agttgacggt gtcattggcg cggttgtgac ggaaacgcca gaagcgatgc tgcaccgcgt
     3601 cgtaggccag gtgcgtggtc gtcgtgaagc cgcctacctc ctgctgcaac acgaaatggc
     3661 tgccgccgag gccgaacatc aggttgttgc ccgttgagtg ccccagcgtg aagtaaattc
     3721 cgaacgagtc tatgcgatga gcggcggaga cttcgatgga ggcgcgagcg tctgtcaaat
     3781 cgacggtggt ggccgacttg tagccatcgt agctggttgc atttgctccc ggcgtaatct
     3841 ccagacggcc gttcgtttcg accatcgtga agtcgggtgc cgcgggtgcg tgccatttgg
     3901 tcgggtcgcg cacgttgtcg ttgaagtcgt cgctgagcgt gatgcctgca tacgcgagca
     3961 ggttcggcga gcctgcgccg gggttgatga ctttgccggg cgtggcggca tttatgatgg
     4021 cctggccaac ggcgtccggc tggtcgcccg gacgcgaggc gagataacgc gcggccagtc
     4081 cggcgacgaa cggcgcggcg aacgacgtcc cggaacgtgg ctggtatgaa gcgttgtcga
     4141 aatgccaggc cgagacgata tccacgcccg gcgcatacag gtcaacgacc gaaccgaagt
     4201 tcgagttggg agattgatgg tcaaactggt cggtggcggc aaccgtgatc gcttccatga
     4261 cccgcgcggg cgaacgcaag gaagcgtcgc ccgtgttgtt gcctgctgcc actactgttg
     4321 tcacacccgc cgcgattaag tttcgcacgg ccagatcaat tgagtcgtta ccgacgatag
     4381 tgatgaagct gaggttagcg accgccggtt tgacgtgatt agctatcacc cagtcgatgc
     4441 ccgcgatgtt gcgcgccgat gagccttgat tctgacagtt gatcacgcgc accgcccaca
     4501 acttcgcgtc cttcgccacg ccgtaggtct tgccgccgat gatccctgcg acgtgcgtgc
     4561 cgtggccgat gcagtcctcg ccgttgcgcc cgtctgcaac gttgtcgtaa gcgaagagaa
     4621 cacgctcgcc gaattcctga tgcttgaagc gaataccgct gtcaataatg taagcgttca
     4681 cgcccgcgcc cgaattggcg tagttgtaag cggcgttgag cggaaggagg cgttgatcga
     4741 tgcggtccag cgcccacgtc gggtcgctct gaacgcccac gccttcgatg agcgtgtctt
     4801 cctcgacgta ttcgacctgc ggattgcggc tgagcgcaag cgcctgcgcc tcgctcaagt
     4861 cgacggagaa gccgcgcagg gcgtgttgat aagtgaaacc gatagtgccg ccgtgcgtgc
     4921 gcgccagttg cgcagcgaag gccttaacgc ggccttgcgg gacgctatct ttgaagacga
     4981 cgatatagtt attgggaatc gcgccggcgc ggcggcggag gcggcctacg gcgtcagatt
     5041 cttgcgtgag cgcgagttgg ggcacgagcg ccagcaggca gagggtcagc aggagaactt
     5101 tgctcagttt cattgaaatt acgtgtaaaa ttttagtgag aagaaacccg gaagaaattg
     5161 cgttagggca cattggccgc tctcacgcct aatcgtctca cacactctcg ccaggacaac
     5221 ctgcatggaa tcgatagtcg tttatcgaaa agttgattgt gagatgtttg agacgatgcg
     5281 agccggagga agcaacactg tccggtgtgt gacaatgccg ccgtgagttc tctgcttaga
     5341 ggtcatccag cttgcgcaat gaataatgca atgagcattc cacacgttcc tcaaactcaa
     5401 agtcggaaag aacaacactt agaggaatcc agagagggga gcgatgaaat ggggtgtttc
     5461 agaatgaaac ttgtgacaca gtcgatggtt cagaataaaa cagggtaatg ttgcaaagat
     5521 tattacgagg cgggaaagac ctacatcaaa gtcgtaaata cctggatcgc gccgtggata
     5581 aggaaggtca aacggccttc cacgcttgaa aataattttc gcaacattac ctttaggtga
     5641 gagcaacgta tcaatgaacc tttttagctt gcgcttctcg tccgttgagc gtgaggatgg
     5701 aatgtgtcac cgtgcctttc ccgtcttcga caaacaagat ttgagcgtcg aattccttaa
     5761 caaagaattc gatgtcggag gcgggcagca actcgatgcg agggaatccg gcggcctgac
     5821 ccatcaactt gtctccttct ttcgtaatcg taatgagaaa ggtgggcgtc agttcatact
     5881 gccccacata accatctaaa atctgcggat tgatgctcgc cactttctga tccttcagcg
     5941 tcgcgccttt ggccccgaag aaaatccccg cgaggtgccg cgccattgtg ttcaccggca
     6001 ggttgtcgaa attgctcagg acgatgacgg tgagtttatc gttgggataa cgcgcaagcg
     6061 cggaggcgaa accaccgatc cagccgccgt ggctgagaca cgtgcgaccg tcctgctctc
     6121 cgatatacca gccataaccg tatccgcctt tatagggcgt gaagatcgca tcaacagacc
     6181 tctttgaaag cagtgtttcc gtggagagcg attgctccca gcgatacagg tcgtctacgg
     6241 tcgaatagag accgcccgca gagaacggca cggacatatc tacatacgac gcgttgacga
     6301 gttgagtggc gcgcaaagaa tagcctgcgg cgcgttgctt caggagccgc tcgtgattgt
     6361 cgtaacccgt gtcgatcatc ttcaggggtt gaaagatatt ttcgtcgaga aacctgtcgt
     6421 aaggcacgcc cgagatactt tcaataatgt aacccagaag gagatagttc gtattcgagt
     6481 atctgaactg cgcccccggc ttaaattcaa ggggctgttc tttaaaggtt tgcacggtgt
     6541 atgcgagcga catcggcgct ttcgcctcga ccgtgtggtc cacattagga agacccgccg
     6601 tgtgagtcag caattgatga atcgtgatgg gttgccaaac tgccggacag cccggcaggt
     6661 atttacagat cgcatcctgc gtttgcaact tgccgcgctg ctgcaatatt aaaattgcgg
     6721 cggcggtaaa ctgtttagtt agtgaaccaa tgcggaattt cgtctccggt gtcaccggca
     6781 cgtcgtgctc cacgttcgcc atgccatacc ctgcgcgcac gattattcgt ccttcgcgcg
     6841 caattagaat cgcgccgctg aaacgactcc ttgtgatcgc gctctttaga tattcgtcaa
     6901 cctgggcacc gacatcctgt gccgggcagg cgacgaaaca tgatgcccag atcacacata
     6961 agagaagcgg ctggcgtaga tattggaggt ttttcttcat aacccggtat taattcccgc
     7021 aaactgttaa atgctgatga ttctcttcac atttgaaacg gggcgctgcc cggcctcgaa
     7081 attacagaag cgtgtgcagt gccccgtttc aaatgaatcc gacacctgcc gaaagttgat
     7141 ccagaccgac tttcgcagaa gtcaggagac gactcttctt acctgtagca gtagccgccg
     7201 ccgggacccg tgttgttggg attgagcgga ccgcgcgagc gtttcgcgtt tgccgcgcag
     7261 caaccggcag cctggaaggc cgctgtgaca gcgcagatca ggttgttgcg gaaattgggc
     7321 gacggggcga cgcaacgctc cgcgaagtgg gaagtggctt tgccgaggca tttgaagaat
     7381 ttcgccagcc agctaaagaa gccgtaagcc atgacgcgct tgaaagaggc cttctggaag
     7441 accgcttcgt tgccgctctt cgttttcccg tccgtcatat acatcgtctc gtaacagagc
     7501 atttggggtt ggtcgtacgt gcgcatcccg ccatcgccgc cgcagccgcc accgccgcca
     7561 ccgccgatcg gttcgccgcc gtcgggcgga tagtagactt cgtcaaactc gtaggccgat
     7621 tcggtgaact cgacgggacc gccgctatcg acgaccgagt tgtaaacgtc agtcgcgccc
     7681 gtcgcatact cggtgacgta gacggttccg tcccagtaag agccactgcc cgaataggtg
     7741 atgaaggaca tctcgccatc ggcgttgctc tggtaggagt cttgcgcgct gaacgagacg
     7801 ttctggattt tcgctcgcgc gtcacgatcc ttttgcgaga tggagggatc aatcgtgaag
     7861 aggacggcgg tttggtccca gtcaacctgt ttccccatct tctccatatc cttcatcgcg
     7921 cggttcagac ctttgtggcg tgcgcgtaaa gccctgacgt gctgcttggc cgcttcgccc
     7981 cggaacgact ccattttctt agctgatttg tctttgccct tgtcctgcac ggtcgcggag
     8041 acgggagcaa tggggatggt gagcgcaaag atagcggctg cgaggacgaa gttcgtcaaa
     8101 cgccgcagag agtttttcat gtgtcaatct ccttcaaact gttaagtaga aacatggggg
     8161 acagggaaaa gacgacttca ccggatggct cacgctttgt cgcgcgaagc cgggagcgcg
     8221 aagttatccg accgtccgtt taatacccac cgagacgatt cagtgtggca cttggcatgg
     8281 aatctgaagt gaggctcggt tgaggattgc cttcttcact tccggttgag ccgtcgcatg
     8341 ttttgcagta agataggagc cgtcatgaac tctgttgtct tctgcgcagt gatgcggcgg
     8401 aggcggcaaa gttaaaaagc tctggtgtta cttggcggta acaccagagc tttttgttgt
     8461 gagcatctca tacgagagat gccgcaaaag caaactccct ggcacagcga cccatcacgc
     8521 aaagccgaag cgatgcatac attttgcaat atacgacgag gcgcagtgaa aaggcgctgc
     8581 ataaaactac cccttgctcg atctgtaaaa gttggacaat cttattggcc ggtgaggcgc
     8641 caataaaaga cttcatttga aatttttaag ggtcgcaact ttcaggctca tacggatggt
     8701 agtaacggaa acggtcatcc tgaaaagttg ttctgcgacg ggcgacagag tagcgtgctg
     8761 gtcgcgcgcc cgtctgtctg aaactcgaca tagcaaaaga aaagttagac atcgggcaaa
     8821 aaaatttgcg caagcatcag gctgccagaa agtggctgat gtcgtggcta acgacccggt
     8881 aacactcgca ggagccgcgt tccagccccg cccggtcaag aattttgatc ttaccgcgac
     8941 tgtatctgat gagcttttgc ccttgaagtt taccggcaat caacgtcacg ctggtgcgag
     9001 ggctgccgag catgtaggcg agaaactcct gcgtcaggtt taaagtatct gctcccacgc
     9061 ggtcgtggct ggtcagcagc caacggcaga gtctcgcctc agcggtgtga aagcgattgc
     9121 agacggctga ctgtgagatt tgcgaaacca gcgtatgtgt gtagcgtaac agcagattgt
     9181 ttaactgttc tccacgcgca aactctctga tgagtagctc ggacttgatc cgcatcgcgt
     9241 ttcccggtat ctgaaccatc acttgataag gagcagagat gcctctcagg atggccgaca
     9301 ccccggccat tccttcattg cctatcatcg ctacttcgac gccgtaaccc tcttccgttg
     9361 tcgaaactag tgaaatcatg ccgccgagcg gaaaataggc ttcacgcacc gggttgcccg
     9421 cgtaataaat gatctttcct ttcttaagcg atgccggttc cagatgtcta ttgaggcgcg
     9481 catattcttc acggggaagg gaggccagaa gtttatttac aaatgccagg ggaggtttat
     9541 tcgaatactg catgcgctga ccttcttttg tgagtgagct tttaaagaag gacacggact
     9601 gctaaggtcc tgatgtcgag aagtgtaagc ccgggtgtgc gggagggcag agtatagccg
     9661 agaatcctgt ttttacacca aaacaataat accttcggat gggtgccgcg ccgccccggc
     9721 ggacgacccg tttggggaaa aggctccgga acctgcggtt cggcaccgaa atgtcggcca
     9781 gcggcgcatg taactccatc gccgccatga cccgcccgcc cctgtttaaa cgcaggcggg
     9841 agcgcactgg ggatttcttc gcacgctgca tcgttcgcta tccggaggga gtccactggg
     9901 ttgcgaaagt gctcgtcgaa tacaggcaag tccacggcgc aggggcggag gggctatgcg
     9961 ccacgaccgt ccgggagccg gagcgcaacc acgctggatg gcagtacgtg atcgaaaagt
    10021 ggcgacgcta tcccaccttt gccgctactg ctgaacggct cgcggcaaag catctagtgc
    10081 tctgtaatgt tagttcttaa agttagcgtt ttgtcgtaat ccatttttgt tagcatctgg
    10141 caattgccat agctcacggc cacaataaaa actatgtttc cagagcacta gcggcggcgc
    10201 ggatcggaaa catccgcagg ctcagcggat cgggtcgaga cggatgcgga agttgaagac
    10261 gttgccccag acgggcgtga agttgcagcc ggtgggcggc aacacccagg agagcgtcgc
    10321 ccgtgccctc aagatcggcc cgttggtgat ggtcgtcgcc gtgctcggcg cggtggtgcg
    10381 ggtgcgcagt ccgccgggga tgttgatgtc gcggtagacg gcgtaattgg tcttgcctga
    10441 accgtcgtgc aggacgacgt tgccctgacc gaccgactcc gtgttctgga acaccccgtc
    10501 gttgttccag tcaacgcaga aactgacata ctcggtcgaa ccgaaggttt ccagcggccc
    10561 gccgtagccg aagttttgct tgatgtcaat gacgcactca agcctcgtct cctgcggata
    10621 gaagccgcag gctctaattt cctcgtaagc cagcgagccg ttgatcgggc cgggcgcgac
    10681 ggcttccgca gagttctgct cagccaacgc ttccgccagc aatttctcca cggttacatg
    10741 gtcgtctctg ctgagagctt cggatatgcg tgcgcggcgc gcgtcttccg gctcctgtgc
    10801 ggtggtttgg gtgacgaacc cggccggcag gatgagaagc gtggcgagga tcaagttgag
    10861 tgagaagcga gacgcggttg ttcgctgggt aaggtttctc atctttcttt tcctttcttg
    10921 ctaaacttta ttcgagaaga gtccgggtca aaattgcttt gaccgctgct gccgtcgagc
    10981 ggtttcagcg atgggataga ctgtctctcc taaagtgtga gtgtgagtag gactgaagcg
    11041 cattcgcaga ggtttctagc tgaaggcgtc ttgcagtcga gtagcgcgca tgaagccgtt
    11101 gaggttgagt tcgcgccggc taaaagtatt cctattcgcc gcgcatgtca atagctataa
    11161 agcgcgggga gacgacgcaa caagagtggc gagaattaag tttcgcctcg cgtcataaaa
    11221 tcatcttgat ccggcctcta gtgctctgta aggttagtgt ttagacttca ctttttcgta
    11281 gtgacggctc aacttcgctc gcgcttgcgt gattgagaac tgccactcga ctttgacttg
    11341 caactcgttt cgctcacaga ccagcacc
//
LOCUS       DASWNS010000111        19591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_472665, whole genome shotgun sequence.
ACCESSION   DASWNS010000111 DASWNS010000000
VERSION     DASWNS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19591
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472665"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VGB76_03735"
     CDS             complement(<1..874)
                     /locus_tag="VGB76_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0098047.1"
                     /translation="MPDLIVNYPDRASDTFPLGRLRITIGRSARNDVCIPDPFASRVH
                     AEVRREGDTYILQDLGSANGTLYNGERVESALTLTPGGRIQIGETEIVFKDKDGGASA
                     TMIADASASLPEATIALNSEQRSTSGLLEAIEDARTQGSAEQPRPNVAGTIIGSARTK
                     NTNSVQQSDLLALISKVSVTLLAPATLDETLRQIVSLVFEAVPAERCLIMLRDQAGEL
                     KIRVAQLRNSKEETGEVRVSRSIIEEVVGQGRSVLTSDAQHDPRFMSSTMTIQGIRSV
                     LAVPLGVGERIFGMI"
     gene            complement(1072..2844)
                     /locus_tag="VGB76_03740"
     CDS             complement(1072..2844)
                     /locus_tag="VGB76_03740"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0098048.1"
                     /translation="MTKTCPSCEILAPPEARYCRHCGTQLKRVGALGDDISPIAATMP
                     LSGQNTTDEIVAPQTPPSADAQTSEVTHEELDDLLQQRGVSPGEGRDAEAERGSDGQY
                     TPARDAVRAGVSTANYTEGANYAEGFRPDPAPGLASDNFDPEQTQITINVRPLTSRNL
                     PADAAASLNPARANSKPQFNPAPQQVTLSPTGSLQPATEMQTTNAAPSPRPASAGSTE
                     SRALRVWLGMGVAILSIVLICGVAFAAFWFGSRAWRNSETPPAITGNPPPPALDPKQL
                     AEAKLAEAAALIASGNAAEAQARLREAAALDPLNAEPQRRLARLLLEGGARREAIEAL
                     RAVTRLAPTDADAWRSLAAAQHAEGLYEDALASYKGLGEASPAALARDTVQLAYADTL
                     RLSGRTSEARAIYRRLAASSNAEVANASRQQLGQPTPEATDEDAEAADAHSDEPTARE
                     TAARAAEEAARTPGTSSALPSPEPASTRSTPPATATTTATPAKASTGSPSEQYQRGIG
                     LWATNRAAAVAEFRAAAERGNSDASYYLGLSIAEGRDPRALKRAELVAAIVYFGRARR
                     GKFRTQSVAYEEQLGRELDRRRNQ"
     gene            complement(2876..3433)
                     /locus_tag="VGB76_03745"
     CDS             complement(2876..3433)
                     /locus_tag="VGB76_03745"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0098049.1"
                     /translation="MTAQMEAGLAERQRRATVERAAAVVFEARRRRVRAPFSLRCGAL
                     LVDYTLAVAILAFTTLLARTLSGGANWTGEAMLATGYLVSLAVLLLNFLVLPVFTGTT
                     VGKWATGLRIERLNGEPLGFGHATLRHTLGYLVSLLTLGIGFLLAAFDAEGRTLHDRI
                     AGTVVVRERGSSSSTSGGTARPPVR"
     gene            complement(3555..5513)
                     /locus_tag="VGB76_03750"
     CDS             complement(3555..5513)
                     /locus_tag="VGB76_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0098050.1"
                     /translation="MSSCPHCGAEVTPTDRFCGDCGTPLPQGKAASQGTAAPGAGMGS
                     AESALAFAVGVEAAPAAVEQRPEAGAEKAASAPREDKGAGARAGESASPISQAQEAFG
                     AAAVKTAGAVEDMSGGAQRAEASAKLGNGADEAAAGHRAEAGGAKEPAAEAARQAENV
                     PAGAEENKRVTGGSDINSSDNRTNAVMGTSRSGSTGRLRHQAKQLDPGTILYSRYEIV
                     RRIGGGGMGAVYLARDRNLGDQPRAVKEMIQSNIDESQHEKAINDFKRESMLLASLEH
                     PSIPTIYDYFYDDEAARFYLVMKYISGGDFLARLRNAPGGRIDEKTVTDWACQVADVL
                     EYLHRRNPPIIYRDLKPANLMIDGNSGRVMLIDFGIARWVTTQEKGVTAVGTMGYAPP
                     ELFSGKVEPRSDIYSLGATMFHLLTGSDPQDNPLLIFDFNKNPRPRQLAPSLSNEMEA
                     ILMRTVGYKPEDRFRSAAELRDALGAHLEKLRAGAVTYGTAHAGDRTGMKTMQVEMVY
                     CGFCGGRIAYDDVYCAHCGQRQPLAVASAALRTARATAKLVVMGTTELDSSFQLHKES
                     SHIGRSDPHSQIFPEVDLSKFDPQTKISRRHARIFRKGDVYLVEDLGSVNGTVLNDSL
                     RLAPHQPRALESGDKLRLGETTLHFLIG"
     gene            complement(5637..6356)
                     /locus_tag="VGB76_03755"
     CDS             complement(5637..6356)
                     /locus_tag="VGB76_03755"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0098051.1"
                     /translation="MSIVGAAEVEIMITCERCQTENVDGAQYCDECGLPLTSAGAAAT
                     GRASADARTPILQMLNKPLTLTDEGASPDVISPDAPTKHDGSKEAADAAASVALSEAA
                     SAVRTSVAARATPAAVTPPRAAGGAHSAHAALVINRGRSAGKEFPVHEDEAYIGRWDA
                     DSGIFPDVDLDSDDPEAKVSRRHARITRRGEQYYIEDLGSTNGTFINRGRRLLPGDRQ
                     PLNDGDEIIIGKTFLRFHVAR"
     gene            6685..8262
                     /locus_tag="VGB76_03760"
     CDS             6685..8262
                     /locus_tag="VGB76_03760"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0098052.1"
                     /translation="MALSRKKKIIIAVSAVAVIALIVIISVFAGRKDEPEVTVVKVTV
                     RPELRSTVTASGEVRPVKFINLTSEVNGRIEEIFVNPGDAVTQGQPLVRLDPTQLQSS
                     QEAQTAGVQVAISDVQNARSSVTAAENSVAQAQQGLTVAEAGLSQARQGVVTSQTSVD
                     RAQVDLNTAQRELRRQTELVEAGVASRADYDAARDRLEQAQVALRTAQAQLQQQRIAV
                     EESRARVNQQKVAVRDAQIGVQRARTGVASSEARVNQQQAILRGQSSQRSKATQLSPL
                     TGVVADIPARQGQFALANLTSTPLMTIADMSTINVEVNVDETEIGNVAVGQAAKVKID
                     ALGEKEVEGIVTQKNPLAVGKSDTQGGGLQNRVNVQEAKEFKVIVELRNMPDDVRNAL
                     RPGMTATANITTKTKQNVLAVPLQAVIEKAPPTPAPAGGGDAAAATPAPAAAPGEKPK
                     EIKGVYVLEGNKVKFLQVETGITGESDIEIVSGLKEGVEVITGPSRVLRTLKENANVK
                     KQTRKPGEGEGKAGESK"
     gene            8266..9030
                     /locus_tag="VGB76_03765"
     CDS             8266..9030
                     /locus_tag="VGB76_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0098053.1"
                     /translation="MSNAIVADRPLEQRIEHGDLLAHDAVPAALISMRDIWKTYQMGS
                     EELHALRGVSFDVQRGEYVAIIGPSGSGKSTLMNLIGCLDTPSQGRYWINSKLVSDMD
                     DDELARIRNKEIGFVFQTFNLLARATALHNVELPLIYAGISSADRHEKAQASLRAVEL
                     GDRMTHRPNELSGGQRQRVAIARALVNHPSLLLADEPTGALDSHTSIEIMNLFEKLHS
                     EGNTIIVVTHEPDIAERAHRVISIRDGNIERDEKIK"
     gene            9391..10629
                     /locus_tag="VGB76_03770"
     CDS             9391..10629
                     /locus_tag="VGB76_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098054.1"
                     /translation="MKLIETLKLAFAAVWAHKLRSALTLLGMIIGVTAVVVVVSLIQG
                     FNRYVDEKIAGIGAKSFTIQRFNIFEDFKDTDTIAAATRRNKDLTMDDFEYLKARAVL
                     IDKLGAGARPSRAQVKRGAEILEEVPIDGMTANVAEINAVEVADGRFFTETENQSAAR
                     VAFLGSDVASKLFPTTSPINEEISIRGLPFRVVGVAVAKGSVLGMPQDNFINIPLKTY
                     GNQFGGFRNNRSLYFVATAKSDELFNDAVEEARQLMRARRRVPANEKDNFGIMTPDAI
                     TGLRDRIFGSIFIVAIAVPAIALVVGGIVIMNIMLVSVTERTKEIGIRKALGARRGDI
                     LKQFLVEAMTLSALGGATGVLLAWIVGRVLTAIFFPTYLSVVAVIVAVTVSGGIGVMA
                     GIIPAWKAARLNPIEALRSD"
     gene            10721..11977
                     /locus_tag="VGB76_03775"
     CDS             10721..11977
                     /locus_tag="VGB76_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098055.1"
                     /translation="MKLLRSDIYENLKMALATLRTNKLRSALTVVGVVIGVWTVMAIA
                     SIISGIDLAVTKEIESFGTRTIILTKFETGIHVGRRSREERMRKELTFEDAMALRALP
                     AVELSVPFLDITNNFFGSKINVSANGKTSSAVRLQGTLPDYERAGIENVSEGRFFSEF
                     ENDQNQQVCVIGASVAENFFPFGSPLEQEIDIGGNKFRVIGIFEKRDQLGGGGGNNDE
                     SNVIYMPYNVARKLKPNADDVFIFAVAKAGQMEAAQDQVVDLMRVRRQTPFGEKNNFG
                     VTTAESAISNFRSITSGVAIAMVVISSVGLMVGGIGVMNIMLVSVTERTREIGVRKAI
                     GARRADILWQFLIEAMTLTGFGGLVGLLLGWGTTFLISIFVPSYVPLWAPIAGFVASV
                     GIGLIFGLWPAWKAARLDPIEALRYE"
     gene            complement(12095..12898)
                     /locus_tag="VGB76_03780"
     CDS             complement(12095..12898)
                     /locus_tag="VGB76_03780"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEY0098056.1"
                     /translation="MSDMNTPISPVNNAVAPPHPAPPAPATETMSTAETLSGIFFEPA
                     RTFEALRSRPRFLIAGIILILAFMAFYILFVQRLGAENIARAQLEARSADMTPEQVEQ
                     ALSIQRQPIVQAITYASFPIVFAVIFAAGAGLYLLGTMAMAKSMSYRQALSVWVYSSM
                     PPLVISSLLSILLLFLKSKDDIDPSAVNQGLARANLGLLVNPKEQPVLGTLLGSIDLF
                     QFYGLFLAAIGLRKVARLSSGAAWGIALAIWLVGVLARVGMSAAFGQAY"
     gene            complement(13061..13558)
                     /locus_tag="VGB76_03785"
     CDS             complement(13061..13558)
                     /locus_tag="VGB76_03785"
                     /inference="COORDINATES: protein motif:HMM:NF015207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEY0098057.1"
                     /translation="MTTVDEDRRQLARVLQGAYSGELAAAYAYRGHWKSLRAGGERER
                     IRQIEDEEWVHRKKVRSIMNTLSVAPVKSRERLMWTIGRTLGLFCHLTGWFLPMYFAG
                     RLESGNVEEYETAAHHARQLELGEFAADLEEMARVEGEHEIFFREMVATHPWLPLMRK
                     FFGWS"
     gene            complement(13659..14753)
                     /locus_tag="VGB76_03790"
     CDS             complement(13659..14753)
                     /locus_tag="VGB76_03790"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY0098058.1"
                     /translation="MIDGTTNERLERVPEAEARAARARDAGEPEARVEELVRVRGLFK
                     HFPVAGSDDVVRAVDGVTFDIFKGETLGLVGESGCGKSTVGRCLLRLIEPTKGEISFE
                     GKDVLALSGKALRGVRAEMQIIFQDPYASLNPRLRVRDIVAEPLVIHGTGTKQERRER
                     VAELLRKVGLDPDYMNRYPHEFSGGQRQRLGIARALALNPKLIVADEPVSALDVSVQA
                     QVINLLEDLQAEFDLTYLFISHGLAVVEHISNRVAVMYLGRIVEVASAVELYANPLHP
                     YTRALLSAIPVPDPTRKRERIVLSGDVPTPINPPSGCRFHTRCPDAIPECSQIDPDLR
                     EVAPGHTVACIRVPGWADAPTLPSVPIKPA"
     gene            complement(14750..15784)
                     /locus_tag="VGB76_03795"
     CDS             complement(14750..15784)
                     /locus_tag="VGB76_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0098059.1"
                     /translation="MSHLLEVKNLQTHFFTRAGVVRSVDGVSFYLDRGELLGLVGESG
                     CGKSVTALSVMRLVGAPGRIVEGEIWFDGEDLRAASEKRMREIRGDDIAMIFQDPMTS
                     LNPVYTVGEQIAEALRLHRKLTRKQAREGAIEAMREVAIPDPARRASDYPHQLSGGMR
                     QRVMIAMALACDPKLLIADEPTTALDVTIQAQILELLDGLRKSRELAVLLITHDLGVV
                     AEVADRVAVMYTGRIVEEAPVAELFARPRMPYTEGLLKSVPKLTVGEAQKVERLQTIE
                     GTVPKPTDLPPGCHFAPRCSYREPRCTVGRIPLYELEGGVKVRCVLYDMASAVAADNQ
                     TAEQMESAGQ"
     gene            complement(15819..16910)
                     /locus_tag="VGB76_03800"
     CDS             complement(15819..16910)
                     /locus_tag="VGB76_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098060.1"
                     /translation="MKKLTSVSALALCATLVCLGQTASLSSAAAQRRRTVASSTASRN
                     DAADAQLTGLYRIDLARSDKLYSVVAGATSNLPFGEQQKFFIDLTVRLTPPDLLAIER
                     RGTRVTLASSRAPRISFEADGLTHNERSGGGQIVSTRATLRADSLSVSVSGGNADKFS
                     VAFEPLEDRRYLRVTRRLNNEQLNEPIIIRSVYEKISDTAQWDIYDTPARPSPTTPER
                     SIASSPAGTNDEASPVAGSNETAFFLRRALEDWIAATNARDIERQMSFYPPVMRAFYL
                     TRNAPRAAVRAEKERVFQRARTVEVRAAAPELIIQEQGRAAVMRFRKSYVIEGGGQNR
                     SGEVVQELRWQLTPQGWKIFSERDVRVIR"
     gene            complement(17044..18696)
                     /locus_tag="VGB76_03805"
     CDS             complement(17044..18696)
                     /locus_tag="VGB76_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY0098061.1"
                     /translation="MSTKTDPVTEPVDNAAAPPKERRARFETSSGVELANDFNPANTD
                     APDYERDLGAPGSYPYTRGVRRNMYRGRFWTMRQYAGYASAEESNARYKYLLAHGTTG
                     LSVAFDLPTQIGLDSDDPLAAGEVGKVGVAIDSLEDMERLFAGIPLDSVSTSMTINAT
                     ASTLLCLYIAVARKQGIAPAKLQGTIQNDILKEYIARGTYIYPPAPSLRLVTDTFAYC
                     AREVPNWNTISISGYHIREAGSTAAQELAFTLADAIAYVEAATAAGLQVDDFAPRLSF
                     FFNAHNNLLEEIAKFRAARRLWARIMRERFQARDPRSLMLRFHTQTAGSTLTAQQPEV
                     NVVRTTIQALAAVLGGTQSLHTNASDEALALPTETAARVALRTQQVIAHESGVADTVD
                     PLAGSYAVEHLTDELERRASDYIKKIDALGGMLRAIELGYVQREIQEAAYNYQRAVET
                     GDATVVGVNRFQIEEDSTPPLLRVEPAIEQSQVERLRALRERRDNASVESRLVALEAA
                     ARTETENLLPHILAAVEAYATVGEISHRLRRVWGEYREAVTV"
     gene            18958..>19591
                     /locus_tag="VGB76_03810"
     CDS             18958..>19591
                     /locus_tag="VGB76_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098062.1"
                     /translation="MFLLPAFPQFPFLSGLGLSLIAPKTVAERPADGQAELNKMQGRI
                     KAAKRLLEREAPETTEIVTVAVADAANSETHLLKLSKAAFLDKDAATVATSSTGKTFK
                     LHPVRPNYVNTAVRVTDAEGREFQPLVVQYPVERDGALTEVGYYSSAHPALSSPELVG
                     GGRAYVHRMLNEAAARLAGHGKVISPEIIDAAERLCIVEHTDHKRFKSEDH"
BASE COUNT         3584 a         6168 c         6195 g         3644 t
ORIGIN      
        1 agatcatgcc gaagatgcgt tcgcccacgc cgagcggcac ggcgagcacg gagcggatgc
       61 cctgaatcgt cattgtcgaa gacatgaagc gcggatcgtg ttgcgcgtcc gacgttaaga
      121 ccgagcgacc ttgaccgacg acctcttcga tgatcgagcg actgacgcgc acctcgcccg
      181 tctcttcttt actgttgcgc agctgggcga cgcgaatctt gagttcgcct gcctgatcgc
      241 gcagcatgat caaacaacgc tcggcgggca cggcctcgaa gacgagcgac acgatctggc
      301 ggagcgtctc gtcgagcgtg gcgggcgcaa gcagcgtgac gctgaccttg ctgatcagcg
      361 cgagcaggtc gctctgctgc acggagttcg tgtttttggt gcgcgccgaa ccgatgatcg
      421 ttcccgccac gttgggtcgc ggttgttcgg ctgagccttg cgtgcgcgcg tcttcgatgg
      481 cttcgaggag gccggaggtg gagcgttgct ccgagttgag cgcaattgtc gcttcgggca
      541 gcgaggcgga ggcgtctgcg atcatggtgg ccgacgcgcc gccgtctttg tctttgaaga
      601 cgatctcggt ttcgccgatc tggatgcgcc cgccgggcgt gagcgtcagg gcggattcga
      661 cgcgctcgcc gttgtagagc gtgccgttgg ccgaacccaa atcctgcaag atgtaagtgt
      721 cgccctcgcg ccgcacctct gcgtggacgc gcgaagcgaa cgggtcgggg atacacacgt
      781 cgttgcgcgc cgagcggcca atcgtgatgc gcaggcgtcc gagcggaaac gtatcggaag
      841 cgcggtcggg atagttaact attaaatcag gcatattctt aaagtgacga gtgacgagtg
      901 acaggtgaca agattaaaac agtaaatcgt gaatcgtaaa tcgtgaatcg aggggaacag
      961 tgaatcgtga atcgcaaatc gtcagtcgtg aatagggtag aacagttttc ctctattgac
     1021 gattcacgat tcacgtcttt tcctgctcgt cactcgtcac ttgtcactgc tttactgatt
     1081 tcttctgcgg tcgagttccc tgccgagttg ttcttcgtag gcgacggatt gagtgcggaa
     1141 cttgccgcgc cgggcgcgcc cgaaatagac gatagcggcg acgagttcgg cgcgtttcag
     1201 tgcgcgcggg tcgcgccctt cggcgatgct caggccgaga taataactgg cgtcggagtt
     1261 gccacgttcg gctgctgcgc gaaactccgc gacggctgcc gcccggttcg tcgcccacag
     1321 accgatgccg cgctggtact gctcggaagg cgaaccggtg gaggctttcg cgggcgtggc
     1381 cgtcgtagtt gcggtcgccg gaggcgtgct gcgcgtgctg gcaggctcgg gcgacgggag
     1441 cgcggaggaa gtgccgggcg tgcgcgcggc ctcttctgcg gcgcgtgcgg ctgtctcgcg
     1501 cgctgtcggt tcgtcgctat gagcgtccgc cgcttctgcg tcttcgtccg tcgcttcggg
     1561 cgtcggttga ccgagttgct gcctgctcgc gttcgccact tccgcattgg aggaggctgc
     1621 gaggcgacga tagatcgcgc gcgcttccga cgtacgaccg gacaggcgca gcgtgtctgc
     1681 gtaagccaac tgcaccgtgt cgcgtgcgag cgcggcgggc gaggcttcgc ccaagccctt
     1741 ataagatgcc agcgcgtctt cgtagagtcc ttcggcgtgc tgcgcagcgg cgaggctgcg
     1801 ccaggcatcc gcatccgtgg gcgcaaggcg cgtcacggcg cgtagagctt cgatggcctc
     1861 acgacgcgcg ccgccttcga gcaacaggcg cgcgagccgt cgctgcggtt cggcgttcag
     1921 agggtcgagc gcggcggcct cacgcagtcg cgcttgtgct tccgcagcgt tgccggaagc
     1981 gatgagcgcg gcggcctcgg ccagtttggc ctccgcgagt tgcttcgggt cgagcgcggg
     2041 aggcgggggg ttgccggtga tggcgggcgg agtttcggag ttgcgccacg cgcgcgagcc
     2101 gaaccagaag gcggcgaagg ccacgccgca gatcaagacg attgacagga tcgccacgcc
     2161 cattcccaac cacacgcgaa gcgcgcgcga ctcggttgac cctgcggacg caggacgcgg
     2221 cgacggcgcg gcgttcgtcg tctgcatctc cgtcgcaggt tggagcgaac ccgtgggaga
     2281 gagtgttacc tgttgcggcg cgggattgaa ttgcggcttg ctgttggcgc gcgccgggtt
     2341 gagagaagcg gcggcgtccg caggcaagtt ccgggaggtg agcggcctga cgttgatggt
     2401 gatctgcgtc tgctccgggt cgaagttgtc ggaggctaaa ccgggcgcgg ggtcggggcg
     2461 aaaaccttcg gcgtagtttg cgccttcggt gtagttcgcc gtcgagactc cggcgcgcac
     2521 ggcgtcgcgc gcgggcgtgt attgcccgtc gctgccgcgt tcggcctccg cgtcacggcc
     2581 ttcgcccgga ctcacgccgc gctgctgtaa gagatcgtcc aactcttcgt gtgtaacttc
     2641 ggaggtctgt gcgtctgcgg agggcggcgt ttgcggagcg acgatctcgt ccgtcgtgtt
     2701 ctggccggag agcggcatgg tggcggcgat gggcgagatg tcgtctccca acgcgcccac
     2761 gcgcttgagt tgcgtgccgc aatggcggca ataacgcgcc tcggggggag cgagaatttc
     2821 gcaactggga caggtcttcg tcatccgtct cctctcaaac ggtgggcggt ggagtttaac
     2881 gcacgggggg acgcgccgtg ccgccgctcg tactgctact gctgccgcgc tcgcgcacga
     2941 cgacggttcc ggcgatgcgg tcgtgcagcg tgcgcccctc cgcatcgaag gcggcgagga
     3001 gaaaccctat gccgagcgtg aggagagaaa ccagatagcc gagggtgtgg cgcagggtag
     3061 catgcccgaa gccgagcggc tcgccgttga ggcgttcgat gcgtaagccc gtcgcccact
     3121 tgccgacggt cgtgcccgtg aagacgggca gcacgagaaa gttcaacagg agcacggcga
     3181 gactcacgag atagcctgtg gcgagcatcg cttcgcccgt ccagtttgcg ccgccgctga
     3241 gcgtgcgcgc caacagcgtc gtgaaggcga ggatggcgac ggccagcgtg taatccacga
     3301 gcaacgcgcc gcacctgagc gagaagggcg cgcgcaccct gcgacggcgc gcttcaaaca
     3361 ccaccgctgc ggcgcgctcc accgtcgccc gcctttggcg ctccgccaac cccgcttcca
     3421 tctgtgctgt cataaataag ctgccggcta taagcttgtg cgctgccagc caaaccataa
     3481 aagagctatt agccggaaga ttcttgtttt agctgatagc taacggctga tagctcatag
     3541 ctcccggctg aaggctaccc tatcaaaaaa tggagcgtgg tttcgccgag gcgcagtttg
     3601 tcgccgcttt cgagcgcgcg tggttgatgc ggcgcgagac gcagagagtc gttcaagacc
     3661 gtcccgttca ccgagccgag gtcttcgacg agatacacgt cgcccttgcg aaagatgcgc
     3721 gcgtggcggc gcgagatttt ggtttgcggg tcgaacttcg acaggtcaac ctccgggaaa
     3781 atttgcgaat gcggatcgct gcgcccgatg tggctcgatt ccttgtggag ttgaaacgac
     3841 gaatcgagtt cggttgttcc catcacgacg agtttggccg tcgcgcgcgc cgtcctcaag
     3901 gccgcgctcg ccacggcgag cggttggcgt tggccgcagt gcgcgcaata cacgtcgtcg
     3961 taagcgatgc gcccgccgca gaagccgcaa tagaccatct cgacctgcat cgtcttcatg
     4021 cccgtgcggt cgccggcgtg cgccgtgccg taagtgaccg ccccggcgcg cagtttttcg
     4081 agatgcgcgc cgagcgcgtc gcgcagttct gccgccgaac gaaaacgatc ttcgggcttg
     4141 tagccgacgg tgcgcatcag gatcgcttcc atctcgtttg agagcgaggg cgcgagctgg
     4201 cgcgggcgcg ggttcttgtt gaagtcgaag atgaggagcg ggttgtcctg cgggtctgag
     4261 ccggtcagga gatgaaacat cgtcgcgccg agactgtaga tgtccgagcg cggctcgacc
     4321 ttgccgctga agagttcggg cggcgcatag cccatcgtgc cgaccgccgt gacgcccttc
     4381 tcctgcgtcg tcacccagcg cgcgatgccg aagtcgatga gcatgacgcg gccactattg
     4441 ccgtcgatca tcaggttggc gggtttgaga tcgcgataga tgatcggcgg gttgcggcgg
     4501 tgcaggtatt cgagcacgtc cgccacctgg caagcccagt cggtgacggt cttttcgtcg
     4561 atgcgcccgc cgggggcgtt cctcaggcgc gcgagaaaat cgccgccgga gatgtacttc
     4621 atgacgagat agaagcgcgc cgcctcgtcg tcgtagaagt aatcgtagat ggtcgggatc
     4681 gaagggtgtt cgagcgaagc cagcagcatc gactcgcgtt tgaaatcgtt gatggccttc
     4741 tcgtgctgcg actcgtcgat gttcgactgg atcatctcct tgacggcgcg cggctggtcg
     4801 ccgagattgc ggtcgcgcgc gagatagacc gcgcccatgc cgccgccgcc gatgcgcctg
     4861 acgatctcgt aacgcgaata gaggatcgtg cccgggtcga gttgcttcgc ctgatgacgc
     4921 agccgacccg tcgagccgga acgcgatgtg cccatcacag cgttcgtcct gttatcgctg
     4981 ctgttgatgt cgctaccgcc cgtcacgcgc ttgttctcct ccgcccccgc tggcacgttc
     5041 tccgcttggc gcgcggcttc cgccgcaggc tccttcgcgc cgcccgcttc cgcacgatgc
     5101 ccggctgccg cttcatccgc accgtttccc aacttcgccg aagcctccgc tcgctgcgcg
     5161 ccgccgctca tgtcttcaac tgcgcccgcc gttttgacgg ctgccgctcc gaacgcttcc
     5221 tgcgcctgcg agatcggcga cgcggactct cccgcacgcg ctcccgcgcc tttatcttcg
     5281 cgcggcgcgg acgctgcctt ctccgctccg gcctccggcc tctgctcgac cgcagcgggc
     5341 gcggcttcga cccccacggc gaaggccagc gccgactcgg cactccccat ccccgcgccg
     5401 ggcgcagccg tgccttgaga cgcggccttg ccttgcggca acggcgtccc gcaatcaccg
     5461 caaaaacgat ccgtcggcgt aacttccgcg ccgcaatgtg gacaggagga catcacttca
     5521 atcttcagga gtcagaactc agaatacaga agtcagcagg aagagcaggc cgcttttatt
     5581 ctgaccactg tattccgact actgttttct gactactgtt ttctgaattc cgacttctac
     5641 ctagcaacat gaaaacgtaa aaacgtcttg ccgatgatga tctcgtcgcc atcgttcaag
     5701 ggttggcggt cgcctgggag caggcgtctg ccgcgattga taaaagtgcc gttggtcgaa
     5761 cccaaatctt cgatgtaata ctgttcgccg cgtcgcgtaa tgcgcgcgtg gcggcgcgag
     5821 actttcgctt ccgggtcgtc cgagtcgaga tcaacgtcag ggaagatgcc gctgtctgca
     5881 tcccaacgcc cgatgtaggc ttcgtcctcg tgcacgggaa actctttccc ggcggaacga
     5941 ccgcgattga tgacgagcgc ggcgtgcgcg ctgtgtgcgc ctccggcggc acgcggcggc
     6001 gtcacggctg ccggggtcgc gcgtgcggcg acggaagttc tgacggcgga ggccgcttcc
     6061 gagagcgcga cggaggctgc ggcgtcggcg gcctctttgc tgccatcgtg cttggtcggc
     6121 gcgtcgggcg agattacgtc aggcgacgcg ccttcgtccg tgagcgtcag aggcttattg
     6181 agcatttgca ggatgggcgt gcgcgcatca gctgaagcgc gccccgtcgc ggcagccccg
     6241 gcggacgtga gcggcaaccc gcactcgtcg cagtattgcg ccccgtcaac attttccgtc
     6301 tggcatcgct cgcaggtgat catgatctcg acttctgccg ccccgactat actcaagaca
     6361 cacggcagat tgtaacgcga aacggaaaag ctagtaaatc gttcaaggcc gtgaatcgta
     6421 aatcgtcaat cgtgaataga cggaaaacgg attttctcca ttcacgattg acgatttacg
     6481 attcataatt cgcggctttt gacaatttac gcgccgacgt ttgagaatta cgagcttttg
     6541 aaactgtttt tgtaattccg acgtaaaagg tttgtcgccg cgagcgttat agttacagca
     6601 gcggttacgc ttcgcgcggc acagttttgc ttttcgcccc gacggttgcc cctgatcccc
     6661 taagttaaag gaagttgaag acgtatggct ctcagccgga agaagaaaat tatcattgcg
     6721 gtttcggcgg tggccgtcat cgcgctcatc gttatcatta gtgtcttcgc cggacgcaag
     6781 gacgagccgg aagtgacggt cgtgaaagtg accgtgcgcc ccgaactgcg ctcgaccgtg
     6841 acggcctcgg gcgaggtgcg accggtcaag ttcatcaacc tgacaagcga agtcaacggg
     6901 cgcatcgaag agattttcgt caaccccggc gacgccgtga cgcaaggcca gccgctcgtg
     6961 cgcctcgacc cgacgcagtt gcagtcttcg caggaagcgc agacggcagg cgtgcaggtt
     7021 gccatcagcg acgtgcagaa cgcgcgctcc tccgtcaccg ccgccgagaa cagcgtggcg
     7081 caggcgcagc aggggttgac ggtggccgag gccggtttgt cgcaggcacg gcagggagtc
     7141 gtcacctcgc agacgagcgt tgaccgcgcg caggttgatc tcaacacggc gcaacgcgaa
     7201 ttgagacggc agaccgaact cgtcgaagcg ggcgttgcct cgcgcgccga ttacgacgcc
     7261 gcgcgagatc gtctggagca agcgcaggtc gctctgcgca cggcgcaggc gcaattgcag
     7321 cagcagcgca tcgcggtcga ggaatcgcgg gcgcgcgtca accagcagaa ggtcgccgtg
     7381 cgcgacgcgc agatcggcgt gcagcgcgca cgcaccggag tcgcttcgag cgaagcgcgc
     7441 gtcaaccagc aacaggccat tctgcgcggc cagtcgagcc aacgctcgaa ggccacgcaa
     7501 ctctcgccgc tcacgggcgt cgtcgcagac atccccgcgc ggcagggaca gttcgccctc
     7561 gccaacttga cctccacgcc cctcatgacc atcgcggaca tgtccacgat caacgtcgag
     7621 gtgaacgttg acgagacgga gatcgggaac gtcgcggtcg ggcaggcggc gaaagtcaag
     7681 atcgacgcgc tcggcgaaaa agaggtcgaa ggcatcgtca cgcagaagaa cccgctcgcc
     7741 gtcggcaagt ccgacacgca gggcggcggc ctccagaacc gtgtgaacgt gcaggaggcc
     7801 aaagagttca aagtcatcgt cgaactcagg aacatgcccg acgacgtgcg caacgctctg
     7861 cgtcccggca tgacggcgac ggccaacatc acgaccaaga cgaaacagaa cgtgctcgca
     7921 gtgcccctgc aagccgtgat cgagaaagct ccgccgacgc ccgcgcccgc cggtggcggc
     7981 gatgcagcgg cggcgacgcc tgcgcccgca gccgcgccgg gcgagaagcc gaaagagatc
     8041 aaaggcgtct acgtgctcga aggcaacaag gtgaagttcc tgcaagtcga gacgggcatc
     8101 acgggcgaat ccgacatcga gatcgtgagc ggactcaagg aaggcgtgga ggtcatcacg
     8161 gggccgagcc gcgtgctgcg tactttgaaa gagaacgcga acgtgaagaa acagacgcgc
     8221 aagcccggcg aaggcgaagg caaagcgggc gagagcaagt aagttatgag caacgctatc
     8281 gtggctgacc ggccactcga acaacgtatc gaacacggcg acctgctcgc gcacgacgcc
     8341 gtccccgccg cgctcatctc gatgcgcgac atctggaaga cttaccagat ggggtcggaa
     8401 gaattgcacg cgctgcgcgg cgtctccttc gacgtgcagc gcggcgagta tgtcgccatc
     8461 atcgggccat cgggttcggg caagtcaacc ctgatgaacc tcatcggctg cctcgacacg
     8521 ccttcgcagg gtcgctactg gatcaacagc aagctcgtct ccgacatgga cgacgacgaa
     8581 ctcgcgcgca tccgcaacaa agagatcggc ttcgtcttcc agaccttcaa cctgctggcg
     8641 cgcgccaccg ccctgcacaa cgtcgaactc ccgctcatct acgccgggat cagttccgcc
     8701 gaccgccacg agaaggcgca ggcatcgctg cgcgccgtcg aactcggcga ccgcatgacg
     8761 caccgcccca acgaactctc cggcggccaa cgccagcgcg tcgccatcgc ccgcgccctc
     8821 gtcaatcatc cctcgctcct cttagcggac gaaccgacgg gcgcgctcga ttcgcacacc
     8881 agcatcgaga tcatgaacct cttcgagaaa ctccattccg aaggcaacac gatcatcgtc
     8941 gtcacgcacg agccggacat cgccgagcgc gcacaccgcg tcatctccat ccgcgacggc
     9001 aacatcgagc gcgacgagaa gatcaagtaa cccgcgaccg actgcgccgg ggcgaccgac
     9061 aataaaaacg cccgccggtt ttgacgaccg gcgggcgttc tcatttgtcc gtttctcatt
     9121 tgtccgtcga agtctatttc accctccggc gacgaggaac acgtcccacg cccgccatgt
     9181 ggggctgtca aacttttacg tcgcagccgc agaatggcgg gcaaagcagg tgcgcaagcc
     9241 ttcctcgaag ccgttaatcc tgcgcctcat ttgacctgcc tctcatccgc aattcataat
     9301 ccgcaggcac aagaaaactg ttttccacgg acggcaggta gcgtactgcc cgtgcaacta
     9361 aaggcgaact tcccccgtaa taatccggtc gtgaaattaa tcgagacttt aaaactggca
     9421 ttcgccgccg tctgggcgca caaacttcgt tcggctctga cgctgctcgg catgatcatc
     9481 ggcgtcacgg cggtcgtcgt cgtcgtctcg ctcatacagg gtttcaatcg ttacgttgac
     9541 gagaagatcg cgggcatcgg cgcaaagtcg ttcaccattc aacgcttcaa catcttcgaa
     9601 gacttcaaag acacggatac catcgccgcc gccacgcgcc gcaacaaaga tttgacgatg
     9661 gacgacttcg agtatttgaa ggcgcgcgcc gtgctcatag ataaactcgg cgcgggcgcg
     9721 cgaccctcac gcgcgcaggt caaacgcggc gcggagatac tcgaagaagt tcccattgac
     9781 ggtatgacgg ccaacgtcgc cgagatcaac gccgtcgagg tggccgacgg gcgtttcttc
     9841 accgagacgg agaaccagag cgcggcgcgc gtcgccttcc tcggctcaga cgtggcctcc
     9901 aagttgtttc ccacgaccag cccgatcaac gaggagattt ccattcgcgg cctgcccttc
     9961 cgcgtcgtcg gcgtggccgt cgcgaaagga agcgtgctcg gcatgccgca ggacaatttc
    10021 atcaacatcc ccttgaagac ttacggcaac cagttcggcg gcttccgcaa caaccgctcg
    10081 ctctacttcg tcgccaccgc caagtcggac gaactcttca acgacgccgt cgaggaagcg
    10141 cgccaactca tgcgtgcgcg ccgccgcgtg cccgccaacg agaaggacaa cttcggcatc
    10201 atgacgcccg atgccatcac gggcttgcgc gaccgcatct tcggctcgat cttcatcgtc
    10261 gccatcgccg tgcccgccat cgcactcgtc gtcggcggca tcgtgatcat gaacatcatg
    10321 ctcgtctccg tcaccgaacg cacgaaggag atcggcatcc gcaaggcgct cggcgcacgg
    10381 cgcggcgaca tcctcaagca atttctcgtg gaggccatga cgctctccgc gctcggcggc
    10441 gcaaccggcg tcctgctcgc gtggatcgtc gggcgcgtct taaccgccat cttcttcccg
    10501 acctacctgt cggtcgtcgc cgtcatcgtc gccgtcacgg tgtcgggcgg catcggcgtg
    10561 atggccggaa ttattcccgc gtggaaggcc gcccgcctca accccatcga agccctgcgc
    10621 tcggactaga aggaagagga atcgtgaatc gtaaatagag ggaagcaggt tttctctgtt
    10681 tccgattcac gactcacgac tcacgattca cggtgtttat atgaaactac tgcgaagcga
    10741 tatttacgaa aacctgaaga tggcgctggc gaccctgcgc acgaataagc tgcgctcggc
    10801 gttgacggtg gtgggcgtcg tgatcggcgt ctggacggtg atggctatcg cttccatcat
    10861 cagcggtatt gacctcgccg tcaccaaaga gatcgaatcg ttcggcacgc gcacgatcat
    10921 cctcacgaag ttcgagacgg gcatccacgt gggtcgccgc tcgcgcgagg aacggatgcg
    10981 caaggagttg accttcgaag acgcgatggc gttgcgcgcg ctcccggcgg tcgaactctc
    11041 ggtgcccttc ctcgacatca ccaacaattt cttcggctcg aagatcaacg tctcggccaa
    11101 cggcaagacc tcttccgccg tccgcctgca agggacgctg ccggactacg agcgcgccgg
    11161 aatcgagaac gtttccgaag ggcgtttctt ctccgagttt gagaacgatc agaaccagca
    11221 ggtgtgcgtt atcggtgcga gcgtggcgga aaacttcttc ccgttcgggt cgccgctgga
    11281 acaggagata gacatcggcg gcaacaagtt tcgcgtcatc ggcatcttcg agaagcgcga
    11341 ccaactcggc ggaggcggcg gcaacaacga cgaaagcaac gtcatctaca tgccctataa
    11401 cgtcgcccgc aaactcaagc cgaacgccga cgatgtcttt attttcgccg tggcgaaagc
    11461 ggggcagatg gaggcggcgc aggatcaggt ggtagacctg atgcgcgtgc gccgccagac
    11521 gccgtttgga gagaagaaca acttcggcgt gacgaccgcc gagtcggcca tctcgaattt
    11581 ccgttcgatc acgtcgggcg tggcgattgc gatggtcgtg atctcttcgg tcggcctgat
    11641 ggtgggcggc atcggcgtga tgaacatcat gctcgtctcc gtcaccgaac ggacgcgcga
    11701 gatcggcgtg cgcaaagcta tcggcgcgcg ccgcgcagac atcctctggc agtttctcat
    11761 cgaggccatg accttgacgg gcttcggcgg cctcgtcgga ctcctgctcg gctgggggac
    11821 gacgtttctc atcagcatct tcgtgccctc ttacgtcccg ctctgggcgc ctatcgccgg
    11881 attcgtcgcc agcgtcggca tcggcctcat cttcggactc tggccggcct ggaaagccgc
    11941 ccgcctcgat cccatcgaag ccctgcgcta tgaatagagg ttagaggtcg gaagttagaa
    12001 ctaagcagca gaggccgtcg agagcgaacc tgcacgcgct ctcgacggcc tctgctttta
    12061 ttgacctccg accttttagc tctgacctct gtttttaata agcctgaccg aacgcagccg
    12121 acatcccgac gcgcgcgaga acgccgacca gccagatcgc gagcgcgatg ccccacgccg
    12181 cgcccgacga caggcgcgcg accttacgca agcctatcgc cgcgaggaag agtccgtaga
    12241 actggaacag atcgatagag ccgaggagcg tccctagcac gggctgttct ttggggttaa
    12301 cgagcagacc taaattggcg cgcgccagtc cctgattcac ggctgacggg tcaatgtcgt
    12361 ctttcgactt caaaaacagc agcaggatac tcaacagcga agagatgacg aggggcggca
    12421 tgctggaata aacccacacg gagagcgcct gcctgtagct catagacttc gccatcgcca
    12481 tcgtgccgag caggtacaac ccggctccgg cggcgaagat gacggcgaag acgatgggga
    12541 aagaggcgta ggtgatggcc tgcacgatgg gctggcgctg gatgctcagg gcttgctcca
    12601 cctgctccgg cgtcatgtcg gctgaacgcg cctcaagctg tgcgcgggcg atgttttccg
    12661 cgccgagacg ctgtacgaac aggatgtaga aggccataaa cgccagtatc aggatgatcc
    12721 cggcgatgag aaaacgcggg cgcgagcgca gggcttcaaa cgtgcgcgcg ggttcgaaga
    12781 agataccgct cagagtttcc gccgttgaca tcgtttcggt ggcgggcgcg ggcggtgcag
    12841 ggtgcggcgg cgcgacagcg ttgttaaccg gggagatcgg ggtgttcatg tcgctcattt
    12901 ttttgtccta cagggattgg gatggagaag actttggtga aagcataacg aacgttgctt
    12961 caagcaagtg acttcaagca gtgacttcgc tctctattac gcacggcgcg aggcgatgtt
    13021 tcattcgaag gtgccgggcg ggaagagagc acgggttcgt ttaactccaa ccgaaaaatt
    13081 tgcgcatcaa cggaagccac gggtgggtgg cgaccatctc gcggaaaaat atctcgtgtt
    13141 cgccttcgac gcgcgccatc tcttcgaggt cggcggcaaa ctccccgagt tcgagttggc
    13201 gcgcgtgatg cgccgccgtc tcatactctt cgacgttgcc gctttcgagc ctccctgcaa
    13261 agtacatcgg caggaaccag cccgtcagat gacagaagag gccgagcgtc cggccaatcg
    13321 tccacatcaa tctctctctg cttttgacgg gcgcaaccga gagcgtgttc atgatcgacc
    13381 tcaccttctt ccggtgcacc cattcctcat cctcgatctg ccggatgcgc tcgcgctccc
    13441 cgcccgccct gagcgacttc caatgaccgc gatacgcgta agccgccgcc aactcgcccg
    13501 aataagctcc ctgcaacacg cgcgcaagct gccgtcgatc ttcgtctaca gtcgtcatct
    13561 cttaaacact atacgaacaa tttttttcgg cgcgcgaata tttttcgagc agtcgggagt
    13621 tcgctcccgg cgtgagttgc cattggcgca cgcgaaactc aagcgggctt gatcggcacg
    13681 gaaggaaggg tcggcgcgtc tgcccaaccg ggcacgcgta tgcaggcgac ggtgtggccg
    13741 ggcgcgactt cgcgcaagtc cggatcaatt tgtgaacatt cgggaatcgc atcggggcag
    13801 cgcgtgtgga agcggcagcc ggaaggcggg ttgatcggcg tcggcacgtc gccggagagc
    13861 acgatgcgtt cgcgcttgcg cgtcgggtca ggcacgggga tcgcggagag cagtgcgcgc
    13921 gtgtaggggt gcagcgggtt cgcgtagagt tcgaccgccg aggccacttc gacgatgcgc
    13981 cccagataca tcacggccac gcggttcgag atgtgttcga cgacggcgag gccgtgcgag
    14041 atgaagagat atgtcaggtc gaactccgcc tgcaaatctt ccagcaggtt gatgacctgc
    14101 gcctgcaccg acacgtcgag cgcagagacg ggttcgtccg ccacgatcaa cttcggattg
    14161 agcgcgagcg cacgcgcgat gccgagccgc tgccgctgcc cgcccgaaaa ctcgtgcggg
    14221 taacgattca tataatccgg gtcgagtcca acctttcgga gcaactccgc gacgcgctcg
    14281 cgccgctcct gtttcgttcc cgtgccgtga atgacgagcg gttcggccac gatgtcgcgc
    14341 acgcgcaggc gcgggttcag gctggcgtag gggtcttgaa agatgatctg catctcggcg
    14401 cgcacgcccc tcagagcctt ccctgagagc gcgagcacgt ccttaccttc aaaggagatc
    14461 tcacccttcg tcggctcgat gagacgcaac aggcaacgac cgacggttga cttcccgcag
    14521 cccgactcgc cgacgagtcc gagcgtctcg cccttaaaaa tatcgaacgt cacgccgtca
    14581 accgcgcgca ccacgtcgtc gctgcccgcg accggaaagt gtttgaacag gccgcgcacg
    14641 cgcacgagtt cttccacgcg cgcttccggc tctcccgcat cacgcgcgcg cgccgcccgc
    14701 gcctccgctt ccggcacgcg ctccaaacgt tcgttcgttg tcccgtcaat cactgtcccg
    14761 cgctctccat ctgttccgct gtttgattgt ccgccgcgac cgccgaggcc atgtcgtaga
    14821 gcacgcagcg aaccttgacg ccgccttcga gttcgtagag cggaatccgt cctacggtgc
    14881 aacgcggttc gcgatagctg caacgcggcg cgaagtggca accgggcggc aggtcggtcg
    14941 gcttcggcac ggtgccttcg atggtctgca aacgctcgac cttctgcgcc tcgcccaccg
    15001 tcaacttcgg cacggacttc aacagcccct ccgtgtaagg catgcgcggg cgcgcgaaca
    15061 actccgcgac cggagcctct tccacgatcc gccccgtata catcaccgcc accctgtccg
    15121 ccacttccgc cacgacgccg agatcgtgcg tgatgagcag caccgccaac tcgcgcgact
    15181 ttcgcaggcc gtctaagagt tccaatatct gcgcctgaat cgtcacgtcg agcgcggtcg
    15241 tgggttcgtc cgcgatgagc aatttcgggt cgcaggcgag cgccatcgcg atcattacgc
    15301 gctgccgcat cccgcccgac aactggtgcg ggtaatcgct cgcgcgtctg gccggatcgg
    15361 gaatcgccac ttcgcgcatc gcctcaatcg ccccttcgcg cgcctgcttg cgcgtgagtt
    15421 ttcgatgcag gcgcaacgcc tccgcgatct gctcgcccac cgtatagacc gggttcaaag
    15481 aggtcatcgg gtcttgaaag atcatcgcga tgtcgtcgcc gcgaatctcg cgcatgcgct
    15541 tctccgaagc ggcgcgcagg tcttccccgt caaaccagat ctccccctcg acgatccttc
    15601 ccggcgcgcc caccagacgc atcaccgaca gagccgtcac actcttccca caacccgact
    15661 cgcccaccaa ccccaacaac tccccgcgat ccagataaaa actgacgccg tccaccgaac
    15721 gcaccacgcc cgcgcgcgtg aagaaatggg tctgcaaatt tttaacttcg agcaaatgag
    15781 acatacgctt gcgagattaa cacatgatca gaaggactct agcggatgac gcgcacgtcg
    15841 cgttcgctga agattttcca gccctgcggc gtgagttgcc agcgtagttc ctgtacgact
    15901 tcgccgctgc ggttctgccc gccgccttcg atgacgtagc ttttgcggaa gcgcatgacg
    15961 gcggcgcgtc cctgttcctg aatgatgagt tcgggcgcgg cggcgcgcac ttcaaccgtg
    16021 cgtgcgcgct ggaagacgcg ctccttctcc gcgcgcacgg cggcgcgcgg cgcgttgcgc
    16081 gtcaggtaga aggcgcgcat gacgggcggg taaaagctca tctgccgttc gatgtcgcgc
    16141 gcgttcgtcg ccgcgatcca gtcttcgagc gcgcggcgca ggaagaaggc cgtctcgttg
    16201 ctcccggcaa cgggagaggc ttcgtcgttc gttcccgccg gggaggacgc gatgctgcgt
    16261 tcgggcgtcg tgggcgaggg gcgcgcgggc gtgtcgtaga tgtcccactg ggcggtgtcg
    16321 gagatctttt catagacgct gcggatgatg atcggttcgt tgagttgctc gttgttgagc
    16381 cgccgcgtga cgcgcaggta acgccggtct tccaacggct cgaaggcgac gctgaacttg
    16441 tccgcgttcc cgccgctgac gctgacgctc aaactgtcgg cacgaagcgt cgcgcgcgtg
    16501 ctgacgatct ggccgccgcc cgatctctcg ttgtgcgtca ggccgtcggc ttcaaaggag
    16561 atgcgcggcg cgcgcgacga agcgagcgtg acgcgtgtgc cccgccgctc gatggcgagc
    16621 aggtctggcg gcgtgaggcg cacggtgagg tcgatgaaga atttctgctg ttcgccgaaa
    16681 ggcaggttgc tcgtcgcgcc cgcgacgacc gagtacaact tgtcgctgcg cgcgaggtct
    16741 atgcggtaca gccccgtgag ttgcgcgtcc gccgcatcgt tgcgagacgc ggtggacgac
    16801 gcgacggtgc gccgtctctg cgccgccgcc gacgagagcg acgcggtttg accgagacac
    16861 acgagcgtcg cgcagagcgc taacgcggat acgctggtta gcttcttcat aaaacctttc
    16921 cccgtttcga catcacgaca ccttgaaacc ttcgactctg gcaacgcact cccgaaaggc
    16981 gtgccgggac tcttaaagct gcttcttcac agtcaataac tgcctcttca cagtcaagct
    17041 ctctcagacc gtcacggctt ccctgtattc gccccagacg cggcgcaggc gatggctgat
    17101 ctcgccgacc gtggcgtagg cctcgacggc ggcgaggatg tgcgggagaa gattttccgt
    17161 ctccgtgcgc gccgccgctt cgagcgcgac gagacgtgac tcgaccgaag cgttgtcgcg
    17221 ccgctcgcgc agcgcgcgca gccgttcgac ctgcgactgt tcgatggcag gttcgacgcg
    17281 caggagcggc ggcgtggagt cttcctcgat ctgaaagcga ttgacgccga ccaccgtggc
    17341 atcgcccgtc tcgactgctc gctggtaatt ataggcggcc tcctgaattt cgcgctgcac
    17401 gtagccgagt tcgatggcgc gcagcatgcc gccgagcgcg tcgatctttt tgatgtagtc
    17461 ggaggcgcgt cgttcgagtt cgtccgtcag gtgttcgacg gcgtaagagc ctgcgagcgg
    17521 gtcaaccgtg tcggccacgc ccgattcgtg cgcgatcacc tgctgcgtgc gcagggcgac
    17581 gcgcgcagcc gtctctgtgg gcagggcgag ggcttcgtcc gaggcgttcg tgtggaggct
    17641 ctgcgtgccg ccgagcacgg cggcgagcgc ctgaatcgtc gtgcggacga cattgacttc
    17701 cggctgctgc gcggtgagcg tggagcctgc cgtctgcgtg tggaagcgca gcatcagcga
    17761 acgcgggtcg cgcgcctgaa aacgctcgcg catgatgcgc gcccacaaac ggcgggcggc
    17821 gcgaaacttg gcgatctctt ccagcaggtt gttgtgcgcg ttgaagaaga aggagaggcg
    17881 cggggcgaaa tcgtcaactt gcagccccgc cgccgtcgcc gcttcgacgt aggcgatggc
    17941 gtctgcgagc gtgaaggcga gttcctgcgc ggcggtcgaa ccggcctcgc ggatgtgata
    18001 accgctgatc gagatggtgt tccagttcgg cacttcgcgg gcgcaatagg cgaaggtgtc
    18061 cgtgacgaga cggagcgaag gcgcgggcgg atagatgtac gtgccgcgcg cgatgtactc
    18121 cttgaggatg tcgttctgga tcgtaccttg aagtttggcg ggggcgatgc cctgcttgcg
    18181 cgcgacggcg atgtagaggc agaggagcgt ggaggccgtc gcgttgatgg tcatcgaggt
    18241 cgagacgctg tcgagcggga tgcccgcgaa gagtcgctcc atgtcttcga gcgagtcgat
    18301 ggcgacgccg actttgccga cttctccggc ggcgagcggg tcgtccgagt ccagcccgat
    18361 ctgcgtgggc aggtcgaagg cgacggagag gccggtcgtg ccgtgcgcga gcagatattt
    18421 ataacgcgca ttggactctt ccgccgaggc atagcccgcg tactggcgca tcgtccagaa
    18481 gcgaccgcga tacatgttgc ggcggacgcc gcgcgtgtaa gggtaagagc cgggcgcgcc
    18541 gaggtcgcgc tcgtagtcgg gcgcgtccgt gttcgcgggg ttgaaatcgt tggcgagttc
    18601 gacgccggaa gaggtttcga aacgcgcgcg gcgttctttg ggcggcgcgg cggcgttgtc
    18661 tacgggttcg gttacggggt cggttttggt gctcatatca atttcgtttc gcagtttgaa
    18721 gtcagcttcg acgccgctct acgacgagca cgctagcaca cggcgcgcgc gcctcgcaat
    18781 gcatgatttt gcgcgccgtc gtcgggcatt ttgaacgccc ctcgactggc aaccggagcc
    18841 tcgttgcgag cgtctgaaga acgtccgccc ccaacacttc cttagtccca agcaacagac
    18901 aaaagaaaga cccgataccc gatgagaaaa ctctttgttt ctgtcctccc tctagttgtg
    18961 ttccttctcc cggccttccc tcagttcccc tttctgagcg gactcggcct ctcgctcatc
    19021 gccccgaaga cagtggccga acgccccgcc gacgggcagg cggaattgaa caaaatgcag
    19081 gggcggatca aggcggccaa gcgtctgctc gaacgcgaag ccccggaaac gaccgagatc
    19141 gtaacggtcg ccgtcgccga cgcggccaac tccgaaacac acctcctgaa actctctaaa
    19201 gccgcttttc tcgacaaaga cgcggccacc gtcgccacct cttcgacggg caagactttc
    19261 aagctgcacc ccgtccgccc gaactacgtc aacaccgccg tccgcgtgac ggacgcggaa
    19321 gggcgcgaat ttcagccgct cgtcgtgcag tatccggtcg agcgcgacgg cgcgctcacc
    19381 gaagtcggct actactcgtc tgcgcatccc gcgctcagtt cgcccgagct cgtcggcggc
    19441 ggcagggctt acgtccaccg catgctgaac gaggccgccg cgcgtctggc cgggcacggc
    19501 aaagtgatct cgcccgaaat tatcgacgcc gccgaacggc tctgcatcgt cgagcacacg
    19561 gatcacaagc gtttcaagtc ggaagatcac g
//
LOCUS       DASWNS010000112         6765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_420431, whole genome shotgun sequence.
ACCESSION   DASWNS010000112 DASWNS010000000
VERSION     DASWNS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420431"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VGB76_03815"
     CDS             <1..309
                     /locus_tag="VGB76_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098063.1"
                     /translation="MSGKILLTYNVEYEVGLDADSLTPARIEEISAQQSIFLCRWLFY
                     LIRQAAVGASSDPFFKPQVFLPAIEAVSLLGEAISHTVNEHVERLSHLQQQAAKRAKK
                     "
     gene            complement(331..2028)
                     /locus_tag="VGB76_03820"
     CDS             complement(331..2028)
                     /locus_tag="VGB76_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEY0098064.1"
                     /translation="MSLTLIALGFLFLLAAAYKFYGTWVARQFATDDARTTPANLVND
                     GVDFVPTRPFYLFAQHFSAIAAAGPIAGPILACQAFGWLPCLLWIGFGVVLIGAVHDF
                     TSLAASVRHGASSIAEITREHLGRRAGAAMMGFIWIALIYVIVAFADITAGTFKSGTE
                     ELRQGGATAFNAGGAVAAASVMYLLLSILLGFVQRYLNPRLWLVTLLFVPATFALSWF
                     GTKVSTTLLLDHKTWGVLILIYCCIASVVPVWTLLQPRGYLGGFILYSALALGVVGVV
                     FGGLSGRYQILQPAFKSWDVGGLTGTLFPFLFVTIACGACSGFHGLVCSGTTSKQVDR
                     ESDIKPVGFGAMLAEGFVALIALVTVMIAAPADIRGKAPGAIYGNGIGQFMTLIIGAD
                     NLQFAITFGAMAFSTFVFDTLDVCTRLGRYIVQELFGWPGRFGALAGTLLTVALPFYF
                     IIYAREGMWTEFWTLFGASNQLLAALTLLAITVWLYQARQRISFTLYPMLFVLTITLW
                     ALCKLAYGNLRAANGFDIKLVNALASAALVILATYLTITALVKLRGERKSSLVPEGA"
     gene            complement(2124..2927)
                     /locus_tag="VGB76_03825"
     CDS             complement(2124..2927)
                     /locus_tag="VGB76_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098065.1"
                     /translation="MNALELIREVYRRNPVLALAGWLHVALLSIFICAAFFDARMVTG
                     VNPWIKPSKFAASFIIYTFTLAWFLPYLSHYRRTVKFISWGTAFVFVGETLCIASQAA
                     RGVPSHFNISTAYDSTVFSLMGLLITFNTLLVLLTLFLFFRQTAPLAPAYVWGIRLGL
                     LLFFLSSVEGYAMASNMAHTVGMPDGGPGLPVVNWSTRAGDLRIAHFLGFHALQILPF
                     AGYSFSRWRADAVRRRAVAFTLAFGLIYFIAFSLLFLQASHGRPLIAWL"
     gene            complement(2914..3363)
                     /locus_tag="VGB76_03830"
     CDS             complement(2914..3363)
                     /locus_tag="VGB76_03830"
                     /inference="COORDINATES: protein motif:HMM:NF025473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HEY0098066.1"
                     /translation="MSLETIFSVCNWTAMLGWLLLIFVPRWKWTARFITAALLPLALA
                     FLYLFLVAAHFAQSEGSFSSLAGVSQLFRNPHNLLAGWIHYLAFDLFIGSWEVRDAQR
                     LGIRHLLVVPCLVLTFIFGPVGLLLYFTLRFVIKREFFVGGSKHERA"
     gene            3733..4143
                     /locus_tag="VGB76_03835"
     CDS             3733..4143
                     /locus_tag="VGB76_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098067.1"
                     /translation="MNQIIIFTYLMYLCISIGLTVWVAQTLFKNGRVFLVDVFRGNEA
                     LADSVNHLLVVGFYLINFGYVSLALRIGYDVTSMRASIEALSWKVGLVLVVLGGMHFF
                     NLYVFSRIRRPQPLFDDSATSMPRTAPRTWVNES"
     gene            complement(4346..5491)
                     /locus_tag="VGB76_03840"
     CDS             complement(4346..5491)
                     /locus_tag="VGB76_03840"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0098068.1"
                     /translation="MKILVLGAGRMGLGAAYDLAHNSDGVAAVTVADIDLERARAVVS
                     TVGSAKLTPAHVDVNDPAQVVALMRGHDAVISCVTYYHNLQLARAAIEARANFCDLGG
                     NNAVVASELALDAEARAAGINIIPDCGLAPGMVSVLAAHGAARFDELEAIHIRVGGLP
                     QSPRPPLNYQIVFSVEGLINEYVERARVIRGGQIVEVESLAEIEALEFPAPYGRMEAF
                     QTSGGTSTLPDTFQGRLQELDYKTIRYPGHCGAFKLFVDLGLAASEEIEVDGVRVRPR
                     RLLGEMLLRHLPADEPDVVLIRLAFRGRTAGRDARLRYDIIDRFDERTGLSAMMRTTA
                     FPASIIAQMMAQGETTGRGALPQELVVPPDKFVAALAARDIRINEQQ"
     gene            complement(5522..>6765)
                     /locus_tag="VGB76_03845"
     CDS             complement(5522..>6765)
                     /locus_tag="VGB76_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0098069.1"
                     /translation="ITNNMKEAYAAWTTAPRLGGIPFVCDVPALGIVFLITALVYIGI
                     RESKRASNVMVALKLAVILLVIVLGAFYVNPANWSPFAPNGASGVLKGVAAVFFAFIG
                     FDALSTTAEECKNPTRDLPRAMIYSLIICTTLYIVLALILTGMVSYKQLAVGDPLAFV
                     FGPEGANMPWMSGIIAVSAVVAIATVLLVFQLGQPRIWMAMSRDGLLPPIFSSIHPRF
                     KTPWFSTIITGLVVAIPSLFMNLTEVTDLSSIGTLFAFCLVCGGVLLGAHTRGLEATP
                     NERSFVPYINSQYIVPAIFILAWAALFYFNPEGLRAFFSLRDPADPTLGVWEVFRHKI
                     PLLAYIILSFVIVYFCFRRRLSLIPVLGLMSCGYLMTELGITNWTRFGLWLVVGLILY
                     FFYGYSHSRLSHPKETTDAAG"
BASE COUNT         1464 a         1919 c         2240 g         1142 t
ORIGIN      
        1 atgagcggga aaatcctgtt aacctacaac gtcgagtatg aagttggact tgatgctgac
       61 agccttacac ccgccagaat agaagaaatc tccgctcagc aatccatctt cctgtgtcgg
      121 tggcttttct acttgattag acaagctgcc gtcggtgcat cctccgaccc gtttttcaag
      181 cctcaagttt tcctgcccgc gattgaagcc gtctcgctgc tcggcgaagc tatcagtcac
      241 actgttaacg aacacgtcga acgcctctct catttacaac aacaagcggc caagcgagcg
      301 aagaagtaaa aggtaaaaag cgtcaatccc ttacgctcct tccggcacga gcgaactctt
      361 gcgttccccg cgtagtttga cgagggcggt gatggttaag taagtcgcga ggatgacgag
      421 ggcggcggag gcgagggcgt tgacgagttt gatatcgaag ccgttggcgg cgcgcaggtt
      481 tccgtaggcg agtttgcaga gcgcccagag cgtgatggtg aggacgaaga gcatcgggta
      541 gagcgtgaag ctgatgcgtt ggcgcgcttg atagagccag acggtgatgg cgagcagcgt
      601 gagcgcggcg agcagttggt tcgatgcgcc gaagagcgtc cagaattccg tccacatgcc
      661 ttcgcgggcg tagatgatga agtagaaggg gagcgcgacg gttaagagcg tcccggcgag
      721 cgcgccgaag cgtcccggcc agccgaagag ttcctgcacg atgtagcgac cgagtcgcgt
      781 gcacacgtcg agcgtgtcga agacgaaggt ggagaaggcc atcgcgccga aggtaatggc
      841 gaattgcagg ttgtccgcac cgatgatgag ggtcataaac tggccgatgc cgttgccgta
      901 gatcgcaccg ggcgctttgc ctctgatgtc ggcgggcgcg gcgatcatca ccgtgacgag
      961 cgcgatgagc gcgacgaacc cttccgccag catcgcgccg aaaccgaccg gcttgatgtc
     1021 cgactcgcgg tcaacctgct tcgaggtcgt gcccgaacag acgaggccgt gaaagccgga
     1081 acacgcgccg caggcaatcg tcacgaagag aaagggaaag agcgtgcccg tcaaaccgcc
     1141 cacgtcccac gacttgaagg cgggttgcag gatttggtaa cgacccgaca agcccccgaa
     1201 gacgacgccg acgacgccga gcgcgagcgc gctgtagagg atgaaaccgc cgagataacc
     1261 gcgcggctgc aacaacgtcc agacgggcac gaccgatgcg atgcaacagt agatgagaat
     1321 cagcacgccc cacgttttgt gatcgagcag cagcgtcgtc gagactttcg tgccgaacca
     1381 ggagagcgcg aaggtcgcgg gcacgaagag aagcgtgacg agccagagcc gcgggttcag
     1441 gtaacgctgg acgaagccga gcaggatcga gagcagcagg tacatcacgc tcgccgccgc
     1501 caccgcgccg cccgcgttga aagccgtcgc gccgccctgt cgtagttcct ccgtcccgct
     1561 cttgaacgtg cccgcagtga tgtccgcgaa ggcgacgatc acgtagatga gcgcgatcca
     1621 gatgaagccc atcatggccg cgcccgcccg gcggcctaag tgctcgcgcg tgatctcggc
     1681 gatggaagac gcgccgtggc gcacggaagc ggcgagactc gtgaagtcgt gcacggcgcc
     1741 gatgagcacg acgccgaagc cgatccacag gaggcacggc agccagccga acgcctggca
     1801 ggcgagaatg ggtccggcga tgggaccggc ggcggcgatg gctgagaagt gctgcgcgaa
     1861 gagatagaag gggcgcgtcg ggacgaagtc cacgccgtcg ttgacgaggt tggcgggagt
     1921 cgtccgcgca tcgtcggtcg caaactggcg cgccacccac gtgccgtaaa atttgtaagc
     1981 ggctgcgagc aggaagagaa atccgagcgc gatgagagtc aggctcatgt gcgaatctcc
     2041 gaatcaaagg acgcgtgcgg aaggatgaag gatgaacttt ttttaacctt ctttaagagc
     2101 gggcgggatt atagaacgaa tctttaaagc cacgctatca agggtcgtcc gtgcgaagcc
     2161 tgtaggaaga gcagcgagaa ggctatgaag tagatgaggc cgaaggcgag tgtgaacgcg
     2221 acggcgcggc ggcgcacggc gtctgcgcgc caacgactga aagagtaacc ggcgaacggg
     2281 agaatttgca gcgcgtggaa gccgagaaag tgcgcgatgc gcaagtctcc ggcgcgcgtg
     2341 ctccaattga cgacgggcag cccgggtccc ccgtccggca tcccgaccgt gtgcgccatg
     2401 ttgctcgcca tcgcgtagcc ttcgacgctg gaaaggaaga agagcaggag tccgagccgg
     2461 atgccccaca cgtaagcggg cgcgagcggc gcagtttggc ggaagaagag aaagagcgtg
     2521 aggaggacga gcagcgtgtt gaaggtgatg agcaatccca tcaggctgaa cacggttgag
     2581 tcgtaagccg tcgagatgtt gaagtgcgac ggcacgccgc gcgccgcctg cgaagcgatg
     2641 cagagcgtct cgccgacgaa gacgaaggcc gtgccccaac tgatgaactt aacggtgcgg
     2701 cgatagtgcg acaggtaagg caggaaccac gcgagcgtga aagtgtagat gatgaaggag
     2761 gcggcgaact tcgagggctt gatccaggga ttcacgcccg tcaccatgcg tgcgtcgaag
     2821 aaggcggcgc agatgaagat ggagagcagc gcgacgtgca gccagcccgc gagcgcgagc
     2881 acagggttgc gccggtaaac ttcccgtatt aactcaagcg cgttcatgtt tacttcctcc
     2941 gacaaaaaac tcgcgcttga tgacaaagcg cagcgtaaag tagagcagca agcccacggg
     3001 gccgaagatg aaggtgagca cgagacaggg cacgacgagc aggtgacgga tgccgagcct
     3061 ctgcgcgtcg cgcacttccc aactgcctat gaagagatcg aaggcgagat agtgaatcca
     3121 accggcgagc aggttgtgcg ggttgcgaaa gagctggctc actccggcga gcgaactgaa
     3181 actgccctcg gattgggcga agtgtgcggc gacgagaaac aggtagagga aggccagcgc
     3241 gagcggcagc aaggccgccg tgatgaaacg cgccgtccac ttccagcgcg gcacgaagat
     3301 gaggagcagc cagccgagca tggctgtcca gttgcacaca gagaagattg tttcgagcga
     3361 catccgcgtt ggctcccgca gtcaaaactt aaaacgtcaa actcacgcga cgggttcgcc
     3421 tctaaaattc ggcgacgtgc cgggcgccgt agtagctcta gcgtccgccg ctccgctgca
     3481 catctctacg agagttcgac cgggcaagtt cgtttccgcg tctctgcgtc tcatggcaaa
     3541 ttgcgaaagc gaatttggcg aatcggcagc ttgccagaag cgcgcgggtg aaagagagga
     3601 aaatcaagga atacggctct ggttcgacgt ggcatgcggg ttgtttacga ctgagggcag
     3661 ccgccgcagt tgaagcccgg caatgaaacc gggagacgcg tggcgacgct tgaaaccacg
     3721 gaaggagaag ccatgaacca gatcattatc ttcacttact tgatgtacct ctgcatcagc
     3781 atcgggctga cggtgtgggt ggcgcagacc ctcttcaaga acgggcgcgt ctttctcgtt
     3841 gacgtttttc gcggcaacga ggcgttggcc gattcggtga atcacctgct cgtcgtcggg
     3901 ttctacctga tcaacttcgg ctacgtgagc ctcgcgctgc gcatcggcta cgacgtgacg
     3961 agcatgcgcg cgagcatcga agcgctctcg tggaaagtcg ggcttgtgct cgtcgtgctc
     4021 ggcgggatgc acttcttcaa tctctacgtc ttcagccgca tccgccgccc gcagccgctc
     4081 ttcgacgact ccgccacttc gatgccgcgc accgcgccgc gcacatgggt gaatgagagt
     4141 taagcgttaa gaatcgagac tcagcgcaga ggcgcggagc agagccgcag cgaacggcac
     4201 agagttaact tctcaactct gtgccgttcg ctgcggcttc gcggcgaatg tttgcactct
     4261 caccttctcc gtctcacttt gttccggctc atcttttccg tctcgttcag gcgcaacccg
     4321 ctctaagttc aggaactctc tcgcctcact gttgctcgtt gatgcggatg tcgcgcgcgg
     4381 cgagcgcggc gacgaatttg tcgggcggaa cgacgagttc ctgcgggagc gcgccgcgcc
     4441 cggtggtttc gccctgtgcc atcatctgcg cgatgatgga ggcggggaag gccgtcgtac
     4501 gcatcatcgc gctcaagccc gtgcgttcgt cgaagcggtc gatgatgtcg taacgcaggc
     4561 gcgcgtctct gcccgccgtg cgcccgcgaa aggcgagacg gatgagcacg acatccggtt
     4621 cgtctgcggg caggtggcgc aggagcatct cgccgagcag gcggcgcggg cgcacgcgta
     4681 cgccgtccac ttctatctcc tcgctcgcgg cgagtccgag atcgacgaac aacttgaacg
     4741 ccccgcagtg gccgggatag cgaatggtct tgtagtcgag ttcctgtagg cggccttgaa
     4801 aagtgtcggg cagggtggaa gtgccgccgg aagtttgaaa ggcttccatc cgcccgtagg
     4861 gcgcgggaaa ctccagggct tcgatctccg ccagcgattc gacctccacg atctgcccgc
     4921 cgcgtatcac ccgcgcacgc tcgacatact cgttgattaa accttcgacc gaaaagacga
     4981 tctggtagtt gagcggcggg cgagggcttt gcggcaagcc gccgacgcgt atgtgaatcg
     5041 cttcgagttc gtcgaaacga gccgcgccgt gcgccgcgag cacggaaacc atgccgggcg
     5101 cgaggccgca atcggggatg atgttgatgc ctgccgcacg cgcctccgcg tcgagggcga
     5161 gttcggaagc gacgaccgcg ttgttgccgc cgaggtcgca gaagttcgcg cgcgcctcaa
     5221 tcgccgcacg agcgagttgc aggttgtgat agtaggttac gcaactgatg acggcatcgt
     5281 ggccgcgcat caacgcgacg acctgcgccg ggtcgttcac gtcaacgtgc gcgggcgtca
     5341 acttcgcgct cccgaccgtg gagacgacgg cgcgtgcgcg ttcgaggtcg atgtctgcga
     5401 cggtcacggc ggctacgccg tcggagttgt gcgcgagatc gtaggccgcg ccgagtccca
     5461 tgcggcctgc gccgagtacg agaatcttca taacgattta acggatgagc agtcgccgtt
     5521 cttatccagc cgcgtcggtc gtctctttag gatggctgag acggctgtgg ctgtagccgt
     5581 agaaaaagta gaggatgagt ccgacgacga gccagaggcc gaagcgcgtc cagttggtga
     5641 tgccgagttc ggtcatcagg tagccacaac tcatcaagcc gagcaccggg atcagcgaga
     5701 gccgccgccg gaaacagaaa tagacgatga cgaacgacaa aataatatag gcgagcagag
     5761 gaatcttgtg ccggaaaact tcccacacgc cgagcgtcgg gtcggcagga tcgcgcaggc
     5821 tgaagaaggc gcgcaagccc tccgggttga agtagaagag cgccgcccac gcgaggatga
     5881 agatggcggg cacgatgtat tgcgagttga tgtagggcac aaaactgcgc tcgttcggcg
     5941 tcgcttccag accgcgcgtg tgcgcgccga gcagcacgcc gccgcagacg agacagaagg
     6001 caaagagcgt cccgatgctt gagagatcgg tcacttccgt caggttcata aagagcgacg
     6061 ggatggcgac gacgaggccg gtgatgatgg tcgaaaacca gggcgtcttg aagcgcgggt
     6121 ggatgctgga aaagatcggc ggcagcagtc cgtcgcgcga catcgccatc cagatgcgcg
     6181 gctgtccgag ttgaaagacg agcagcaccg tcgcaatcgc gacgaccgcg ctcactgcga
     6241 tgatgccgga catccagggc atgttcgcgc cttcgggtcc gaacacgaaa gccagcgggt
     6301 cgcctacggc caactgtttg taactgacca tgcccgtcag gatgagcgcg agcacgatgt
     6361 agagcgtcgt gcagatgatg agcgaataga tcatggcgcg cggcagatcg cgcgtcgggt
     6421 ttttacattc ttcggcagtg gtggagagcg cgtcgaaacc gatgaaggcg aagaagaccg
     6481 ccgccacgcc cttcaacacg ccgctcgccc cgttcggcgc aaacggactc cagttcgccg
     6541 ggttgacgta gaacgcgccg agcacgatga cgagaagaat gacggcgagt ttcagcgcga
     6601 ccatcacgtt cgaggcgcgc ttcgattcgc gtatgccgat atagacgagc gcggtgatca
     6661 ggaagacgat gccgagcgcg ggcacgtcgc acacgaaggg gatgccgccg aggcgcggcg
     6721 cggtcgtcca ggccgcgtaa gcctctttca tgttgttcgt gatgt
//
LOCUS       DASWNS010000113        15024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_315564, whole genome shotgun sequence.
ACCESSION   DASWNS010000113 DASWNS010000000
VERSION     DASWNS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15024
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315564"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="VGB76_03850"
     CDS             complement(<1..1687)
                     /locus_tag="VGB76_03850"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEY0098070.1"
                     /translation="MRSLDAAASLSLALILLLVSMPLSQTGAASLPPEYDLVIVNGLV
                     VDGTGRPAYRADVAVRGDRIETITQVRRIPRGRRVIDAKGLVVAPGFIDMLGQSEQNL
                     LIDPRAMSKVMQGVTTEVTGEGGSIAPLNARLIKEDEDFYRRFNLTADWRTFAEYFRR
                     VERQGLGLNLATFVGATQVRAYAMGYENRAPTPAELQHMRRLVAQAMEEGALGLSTSL
                     QYVPARFAKTAEIVELAKVARRYGGVYATHQRSEANALDSSLAEVFEIARRARIPTEI
                     WHLKTAYKKNWGRMPEVLRRIEAARAEGLQITADVYPYTAASTSLTACLPPWALEGGV
                     EKMLARLGDPETRERLKRDISTDAKDWENIYLGSGGAEGVLIGSVVKRELEALQGQRL
                     SQIAAAQNKDALDALFDLVVADRGQTGAIYFMMSEDDLRAALRSPFVSICTDSGARAT
                     DGPLSGTKSHPRGWGSYPRILSRYVRDEKLLTLEQAVHKMTGMSAARVGLRERGVLRP
                     GAFADITIFDPQRVRDRATFEQPNQYAEGINYVVINGQLAVENGQRTTALAGRV"
     gene            1859..3376
                     /locus_tag="VGB76_03855"
     CDS             1859..3376
                     /locus_tag="VGB76_03855"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY0098071.1"
                     /translation="MPDAEGRKRGATLADVARATGGKLSGADGDTAVVSDVTHDSREA
                     RAGSLFVAIRGAKFDAHSFVSQVLEQGAVGIISERARPDDFAGAWIEVADARVALAQA
                     AATVQGHPSRELKLVGITGTNGKTTTAHLLAAVCEAAGERVAVLGTVGYRIGEERFKA
                     EHTTPEASAIQRFLRRAVEAGCSVALMESSSQALDLRRCDALHYSVAVFTNLTRDHLD
                     YHGTMEDYYQAKLRLFDGRLGTPPQISVINLDDPYGVRLASELGERGARSITYALDAE
                     ADVSAHAVEYRLEGMRFSLRARAFESHVNSPLVGRPHIYNMLAATATGLALGYDPVVM
                     VRAFETCAGAPGRFERVAHTTGDFAVVVDYAHTDDALKNVLRTARDVVTGEGRVITVF
                     GCGGDRDRTKRAPMGEVAASLSDIVILTSDNPRTEDPEAILADTEVGLRTVNKPYVKI
                     ADRRAAIFRAIAEAHDGDVVVIAGKGHEDYQIIGTETIHFDDREVAREALAAREN"
     gene            3398..3961
                     /locus_tag="VGB76_03860"
     CDS             3398..3961
                     /locus_tag="VGB76_03860"
                     /inference="COORDINATES: protein motif:HMM:NF022215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HEY0098072.1"
                     /translation="MRMRRFWPWLALAAIMVAAVLQLRRQGRLWSCACGRVLLWSGDA
                     WSADNSQHLFDPYSFTHVLHGFLFCGLLAWLLPRLSAVWQLTLASAIEAVWEVLENSA
                     FIINRYRENTLALGYTGDTIINSFGDMLACGVGFMLARRLGLRRTLLIFLLTEIALLI
                     WIRDSLILEILMLIHPVDAIKAWQVSH"
     gene            complement(4049..4180)
                     /locus_tag="VGB76_03865"
     CDS             complement(4049..4180)
                     /locus_tag="VGB76_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098073.1"
                     /translation="MIYQIEFKPRAVKDMKSFTVEDRRRIMSKIEGLQNNPAAMLSD"
     gene            complement(4196..5650)
                     /locus_tag="VGB76_03870"
     CDS             complement(4196..5650)
                     /locus_tag="VGB76_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0098074.1"
                     /translation="MSTQPATDNVKLIRGLTLLAAVSIVIGNVIGTGVFLKARVMTCN
                     VGTPYMVVIVWIAAGILSLAGALTYAELAAMMPRAGGEYVFVREAYGGRLGFLYGWMQ
                     IFIAKTGSQASVAVAFAIFLNILTGGALNATFFTIPLGSFSLPFGYLQVVALSAIAIV
                     TIINCAAVVVSGHIATVLTGVKIALVLFVGLGALLFAGGGDWSHFGMANAGGACEGVS
                     AGARAGIAGFGAAMLGALGGYDGWNNLTLVAGEVKNPQRNIPLALIGGMITIISLYVF
                     VNIAYFYALAPTEVASVPAASSVASEVARTFLGPFAVSLIAAALLFSSVGTLHTSILT
                     GARVPYAMARDGLFFQNLARLSPKTHVPIGALILQGIWAGILALSGSFDTLTDYVIFG
                     SWIFYGLTTASVFVFRRRMPHAERPYRAWGYPVVPILFLIVTGGLLVNTLLTTPKQAL
                     IGLGLILLGLPVYWYWSKHNRAVNRSSETADVQE"
     gene            complement(5759..6259)
                     /locus_tag="VGB76_03875"
     CDS             complement(5759..6259)
                     /locus_tag="VGB76_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEY0098075.1"
                     /translation="MSQRRNLFAVVILALIFVGFPARHNAQTPATANTDEAAVRQIIQ
                     QVQDGWNAHDGKAFAAPFATDADYVVVNGMYAKGRETIERGHTAIFSTIYKESRNVAT
                     VKSVRFLRPDVAVAHVEWNLEFKMNGATQKGHAINTMILTKEGGKWSIAAFQNTPVQP
                     QQEHRP"
     gene            complement(6405..7814)
                     /locus_tag="VGB76_03880"
     CDS             complement(6405..7814)
                     /locus_tag="VGB76_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0098076.1"
                     /translation="MNTKKTLSNRRAFLKSFGAAALGLPLLLDSSSGQTQRHRGKHSC
                     IVVGAGLSGLAAAYALKRANWDVTILEARARHGGRVHSFRFDQNPELVCELGGEWIGA
                     SHERMRALCREFALPLKDHRFEATLMRDARVSPPGSWGFSAQAEAVFELFKKRYHHYT
                     ERERRRLDQLDWWTWLEQLGFPEDDLLLRDLQDSTDFGESIRHVSAYAAASEYLESSP
                     ANEMDFKIVGGNSRIIEEFAKRIGHDSIRTRAQVEEIRQRAGRVTVKAGGVLYQADAC
                     ICTVPARVLDQIKFDPPLPAAQSAAAEQLQYSRIVKNQILFDERFWGAEDFSLVSDVT
                     SHYYFHSTRDQRGTQGILCSYAVGEKADVLAAQSDQRRMEIITRDLLPFNERATQLAR
                     SIQSMPWQRDRYTQGAYAVYRPGQWFTVRPTLQKPHGKVIFAGEHLADWQGFMEGAVV
                     TGEAAAKALTGTARARRRA"
     gene            complement(7852..9273)
                     /gene="lysA"
                     /locus_tag="VGB76_03885"
     CDS             complement(7852..9273)
                     /gene="lysA"
                     /locus_tag="VGB76_03885"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY0098077.1"
                     /translation="MPAQTPAPPAPAWTIHGYLEAHEGRLHIDGADACELVREYGSPL
                     YVFSEPRIRSNIARLQQAAASTRRPFKFCYASKANSNMAVLKAVREAGIDIEVNSGGE
                     LFKALRAGFHPAQIIFNGTSKTDDELDDAVRAEIYSINVDSVYEIELVEAAARRVGKR
                     ANITLRLVPEIGTRSHIGLQTALLTSKFGITSTEVPAAFRRALMHPRLIRVGGIHIHI
                     GSQTPDVEPFAAAFKAMWEHLVALHRETGHMLEHINLGGGIPVNYLRDRSQADTLPAA
                     ERDMLGASLEPAEVLDAALRVARESARATEAEHLLDRLTILLEPGRSVIADAGLVLTT
                     VRNIKRRPETGDVWLLTDAGFNLLLSMTTYKWYYHLISASRADAPPDTGYKVAGPLCD
                     SGDVYFDLEGENRLPDYRRLPAEVRPGEVLALLNSGAYSLAQAFPYNGRPLPAAVMIG
                     EGGHARLVRRRDTYEDLLTGDIW"
     gene            9488..10615
                     /locus_tag="VGB76_03890"
     CDS             9488..10615
                     /locus_tag="VGB76_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0098078.1"
                     /translation="MDKAFDWQRVREEFPVTERLAYLNSAGAGPVPRAASDAALEFYR
                     EMLESGDKRWFDWLERRERARATISQLINAEPEEIAFTINTSTGMNLIIDALEGRGEV
                     VSCELEFPVSTITWMHRGMRVHLLKARGGEVSVEDVSAAIASRRTGVICLSHVQYSNG
                     FRAPLAEIGARKGEHAFVVNAAQSAGVFQIDVKRMKIDALCATGHKWMLGGFGSGFVY
                     LSRALLEATRPRAVGWMSEEHPFEMRNDEFHVRGDAAARAEMGCPHFAGIFSLGASAN
                     YMLQLGTGEIERRALALNRMLTEQLAAAGWRVLSPLRDEATRSAETLVAADDPARVAR
                     HLSTRGVAVTQKPEGIRVATHFFNNEEDIARLVAALDEVRS"
     gene            10680..11249
                     /locus_tag="VGB76_03895"
     CDS             10680..11249
                     /locus_tag="VGB76_03895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0098079.1"
                     /translation="MSLERPLFIPVILGTPRQGRMSEHAARFVFGELEKRAGIETEWI
                     DVRELNLPTTDAGEAIKDAGFSRACARADALVIVVPEYNHGYPGLLKHALDTNLKEYI
                     HKAVGVCGVSAGGFGGTRVIEHLLPVLRELGLVAIFNDVNFSRVSKIFDESGALLDES
                     YVKRTKSFLDELVWMAKVLRYGRENVVVE"
     gene            11400..13481
                     /locus_tag="VGB76_03900"
     CDS             11400..13481
                     /locus_tag="VGB76_03900"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEY0098080.1"
                     /translation="MSSVHDASAPVAAAATYLNPVYGRDFPDPFILKHAGEYWAYATG
                     FWRDGRAFGVLHSRDLVHWRELEGALAPLPGEHPCYWAPEVTYENGRFLMYYSVGNEK
                     RMHIRVAVAEHPAGPFVDSGHQLTDAEFAIDPHVFIDVEGTRWLFYATDFLTHTHIGT
                     GTVRERMLDPFTLAGDTRPVTRARYDWQVYDPARQEKGGVRWHTVEGPFVLRRKNLYY
                     QMFSGGNWQNLTYGVSYAVSDRIEREDEWEQAADGARVLPILRTIPERVIGPGHNSVV
                     RGTDNRELFCVYHRWDGEHGRVLAIDRLDWVGERMTVLGASTTPQPAPAPPTFAGFYG
                     EHEDGLGEGWTCTTQGGAQWSVREGEARQETVAEGARASARCDARAASFILEVSLRAL
                     DAEAQRGAFGVALLRDDEESALRFLLEPETGEARVDWQAEDGTRADVRFALPSDFAPR
                     AFHLLRVVVDNACVTISLDERVVRWQGTLKAAPDSAALVTTEGARAAFKGFELTHGWE
                     ELFTQTERTLAARGWQHNSEGWHVQDDLLRFDDREGRPNAAIVKGTLLESYELVVNVR
                     LEPEPAPGNGCYGFYPAYVVGAAEQEQLITVEREGGAEGECALVLRSLRDDSNRESWP
                     LPASFDPSVMQQFRFRKQGGRLAIWWEAAPLGEISTTTEATAIGLYAQRARVACDMVR
                     VTALRTVTSDK"
     gene            13569..14951
                     /locus_tag="VGB76_03905"
     CDS             13569..14951
                     /locus_tag="VGB76_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY0098081.1"
                     /translation="MTELILGTRPEDFIWASGVEDSFVPQVKAGHRALDEYQLMGHYE
                     HWREDLALGRELGVRALRWGVPWYRVEPEPGVFDWRWTDEVLPYMIEELGITPIVDLM
                     HYGCPLWLKKEFISKDYVRAVSAYAAAFARRYKDSVKWYTPLNEPLVNALMCGKRGLW
                     PPYLRGDVGYIRIMLQLVRGIIATVEAIKEVDPLSIMVHVEATGLSRAIRQDLESLAA
                     EEQRRGYLAYDLLVGSITHDHPLFPWLLRSGASVDMLDEIARHKISLDVLGMNFYPQW
                     STQHLYVDPKGRLAYRAHEEDGVGFAAVINDYYQRYKAPIIITETSAFGSEELRSRWL
                     KSSVEAVKYLRSQRIPVVGYTWFPMFTMIDWRYRHSRAPLEHFHLELGLYRLNSKGDA
                     STPAQGRWSATPLVEQVRAYITDSVSSVGALDPRALAPALNTEACKAHARTPTPPDAG
                     TPAPEQMPQG"
BASE COUNT         2760 a         4460 c         5010 g         2794 t
ORIGIN      
        1 ggacgcgccc ggcgagagcc gttgtgcgct ggccgttttc gacggcgagt tgaccgttga
       61 tgacgacgta gttgatgcct tcggcgtact ggttcggctg ctcgaaggtg gcgcggtcgc
      121 gcacgcgttg cgggtcgaag atggtgatgt cggcgaacgc gccggggcgc agcacgccgc
      181 gttcgcgcag cccgacgcgc gcggcggaca tgccggtcat cttgtgcacg gcctgttcga
      241 gcgtgagcag tttttcgtcg cggacgtagc gcgagaggat gcgcgggtaa gaaccccagc
      301 cgcgcgggtg cgacttcgtg ccggagagag gtccgtcggt ggcacgcgcg ccgctgtcgg
      361 tgcagatgga aacgaagggg gagcgcagcg cggcgcgcag gtcgtcttcg gacatcatga
      421 aatagatcgc gccggtctgc cctctgtccg cgacgacgag atcgaagagc gcatcgagcg
      481 cgtccttgtt ttgcgcggcg gcgatctggg agagacgctg cccctgcaag gcttcgagtt
      541 cgcgtttgac gaccgagccg atgagcactc cctccgcgcc gccgctgccg agatagatgt
      601 tctcccaatc tttcgcgtcg gtcgagatgt cgcgtttgag tcgctcgcgc gtctccgggt
      661 cgccgaggcg cgcgagcatc ttttcgacgc cgccttcgag cgcccacggc ggcagacagg
      721 cggtgagaga agtgctggcg gcggtgtaag ggtacacgtc tgctgtgatc tgcaaacctt
      781 ccgcgcgcgc cgcctcgatg cgtctcaaca cttcgggcat ccgaccccag ttctttttgt
      841 acgcggtctt gagatgccag atctccgtgg ggatgcgcgc acgccgcgcg atctcgaaga
      901 cttccgcgag cgaactgtcg agcgcgttgg cctcgctgcg ttggtgcgtg gcgtagacgc
      961 cgccgtaacg gcgtgcgacc tttgccagtt cgacgatctc tgcggtcttg gcgaagcgcg
     1021 cggggacgta ttgcagcgaa gtggagaggc cgagcgcgcc ttcttccatc gcctgtgcga
     1081 cgagacggcg catgtgttgg agttcggcgg gcgtgggcgc gcggttctcg tagcccatcg
     1141 cgtaggcgcg cacctgcgtc gcgccgacga acgtggcgag gttcaagccc aagccctgac
     1201 gctcgacgcg ccggaagtat tcggcgaagg tgcgccagtc agccgtgaga ttgaagcggc
     1261 gataaaaatc ttcgtcttcc ttgatgagcc gcgcgttcag aggcgcaatc gagccgcctt
     1321 cgcccgtcac ctccgtcgtc acgccctgca tcaccttgct catcgcgcgc gggtcgatca
     1381 agaggttctg ttcggattga ccgagcatgt ctatgaaacc gggcgcgacg accagcccct
     1441 tcgcgtcgat cacccgtcgc ccgcgcggga tgcgacgcac ctgtgtgatc gtctcgatgc
     1501 ggtcgccgcg cacggccacg tcggcgcggt aggcagggcg accggtgccg tcaacgacga
     1561 ggccgttgac gataacgaga tcatattcgg gcgggagact cgccgcgccg gtctgcgaga
     1621 gcggcatcga gacgagcagc aggataagcg cgagcgacag actcgcggcg gcgtcgagtg
     1681 atctcattaa tcctccgaga gagaatggac gtgtgcgcgg cgtcgcatgg taatgctttc
     1741 gcgccgggga tgtaaactat cttgaaaaaa ttcgtgcacg acattgcggc ggccagcgac
     1801 ggatgtaatt tataatcaaa tgcaacaggc gagagagaaa ggatgagttg aaaatttgat
     1861 gccggatgca gaaggacgga aaagaggcgc gacgcttgca gacgtggcgc gtgcgacggg
     1921 cgggaaactt tcgggcgcgg acggcgacac ggcagtcgtc tctgatgtca cacacgattc
     1981 gcgcgaggcg cgcgccggga gtctcttcgt agcgattcgc ggcgcgaagt tcgacgcgca
     2041 cagtttcgtc agtcaggtct tggagcaggg cgcggtggga atcatctcgg agcgcgcgcg
     2101 tccggacgac tttgcgggcg cgtggattga agtcgcggac gcgcgcgttg cgcttgcgca
     2161 ggcggcggcg acggtgcagg ggcatccctc gcgcgaactc aaactcgtag gcatcacggg
     2221 cacgaatggc aagacgacga cggcgcacct gctggctgcc gtgtgcgaag cggcgggcga
     2281 gcgcgtggcg gtgctcggca cggtcggcta tcgcatcggc gaagaacgat tcaaggccga
     2341 acacacgacg cccgaagcga gcgcgattca acgcttcctg cgccgcgcgg tggaagccgg
     2401 atgcagcgtt gccttgatgg agagttcgtc gcaggcttta gacctccgcc gctgcgacgc
     2461 gctccattac agcgtcgcgg tcttcaccaa tctcacgcgc gatcatctcg actatcacgg
     2521 cacgatggaa gattactatc aggccaagct gcgtctcttc gacggacggc tcggcacgcc
     2581 cccgcaaatt tccgtgatca acctggacga tccttacggc gtgcggctcg cctccgaact
     2641 cggagagcgc ggggcgcgct ccatcaccta cgcactcgat gcggaggcag acgtgagcgc
     2701 gcacgcggtt gaatacaggc tcgaagggat gcgcttttcg ttgcgcgcgc gggcgttcga
     2761 gtcgcacgtc aattcgccgc tcgtcgggcg accgcacatc tacaacatgc tcgcggcaac
     2821 cgccaccggc ctcgcgctcg gttacgaccc tgtcgtgatg gtgcgcgcct tcgagacttg
     2881 tgcgggcgcg cccggtcgct tcgagcgcgt cgcgcacacg accggcgact ttgccgtcgt
     2941 cgtcgattac gcgcacacgg acgacgcgct gaagaacgtc ctgcgcacgg cgcgcgacgt
     3001 ggtgacggga gaggggcgcg ttatcacggt cttcggttgc ggcggcgacc gcgaccgcac
     3061 gaagcgtgcg ccgatgggcg aggttgccgc ctccctgagc gacatcgtga tcctcacctc
     3121 tgacaacccg cgcacggaag accccgaagc gattctcgcg gatacggaag tcggcctgcg
     3181 aaccgtcaat aaaccctatg tgaagatcgc tgatcgccgc gcggccatct ttcgcgcgat
     3241 tgccgaagca cacgacggcg atgtcgtcgt catcgcaggc aaggggcacg aggactacca
     3301 gatcatcgga accgagacga tccatttcga cgaccgggaa gtggcgcgcg aggctctggc
     3361 cgcacgtgaa aattaaggac gcgtgaaaat taaacggatg cgtatgagaa gattctggcc
     3421 gtggctcgcg ctcgcggcga tcatggtcgc cgccgtgctc caacttcgta gacagggacg
     3481 cctgtggtct tgcgcgtgtg gccgcgtgct gctctggtcg ggcgacgcgt ggagtgcgga
     3541 caactcccaa cacctgttcg acccttacag cttcacgcac gtcctccacg gcttcctctt
     3601 ttgcggcctg ctggcgtggc tgttgccgcg tctctctgcg gtttggcaac tcacgctcgc
     3661 cagcgcaatc gaagcggtgt gggaagtttt agaaaactcc gccttcatca tcaaccgcta
     3721 tcgtgaaaac acactcgcgc tcggctacac gggcgacacc atcatcaatt ccttcggcga
     3781 catgctggcc tgcggcgtcg ggttcatgct ggcacgccgt ctcggcctgc gccgcacgct
     3841 cctcatcttc ctgctcaccg aaatcgcact cctcatctgg atcagagaca gcctcatcct
     3901 cgaaatattg atgctcatcc acccggtaga cgcgatcaag gcgtggcagg tgagccacta
     3961 ggcgggaatg ggaaattttt gtcgtccgct gtctcaaata atattcgata gtctcccacc
     4021 ggcagacggt acccgggcgt gaagttcgtt agtcgcttaa catcgccgcc gggttgtttt
     4081 gcaatccttc gattttagac atgatccggc gtctgtcttc cacggtgaaa gacttcatat
     4141 ccttgacggc gcgaggcttg aactcgatct gatagatcac ttgtcattcc tgcgattatt
     4201 cttgcacgtc tgctgtctcc gaacttcgat ttacggcgcg gttatgtttc gaccagtacc
     4261 agtagacggg caggccgagg aggatcagtc cgaggccgat gagggcttgt ttcggtgtgg
     4321 tcaggagcgt gttgacgagc agcccgcccg tgacgatcag gaagaggatg gggacaaccg
     4381 gataacccca cgcgcgatag ggacgctcgg cgtgcggcat gcggcggcgg aagacgaaga
     4441 cggaagccgt ggtgagtccg tagaaaatcc atgagccgaa gatcacgtag tcggttagtg
     4501 tgtcgaagga gccggagagg gcgaggatgc ctgcccagat gccttgcagg atgagcgcgc
     4561 cgatggggac gtgggttttc ggcgagaggc gcgcgaggtt ttgaaagaag agtccgtcgc
     4621 gcgccatcgc gtaggggacg cgtgcgcccg tcaggatcga agtgtggagc gtgccgacgg
     4681 aagagaagag gagcgcggcg gcgatgagac tcacggcgaa ggggccgagg aacgtgcgcg
     4741 ccacttccga ggcgaccgaa gaggcggcgg gcacgctcgc cacttcggtc ggcgcgagcg
     4801 cgtaaaagta tgcgatgttg acgaagacgt agagcgagat gatcgtgatc atgccgccga
     4861 tgagcgcgag cgggatattg cgttgcgggt tcttcacttc gcccgcgacg agcgtgagat
     4921 tgttccagcc gtcgtagccc ccgagcgcgc cgagcatggc cgcgccgaac cctgcgatgc
     4981 ccgcgcgtgc gcccgcgctc acgccctcac atgcgccgcc cgcgttcgcc atcccgaaat
     5041 gcgaccagtc gccgcccccc gcgaacagca acgcgccgag gccgacaaag agcacgagcg
     5101 cgatcttcac gcccgtcagc accgtcgcga tatggccgct gacgaccact gcggcgcagt
     5161 tgatgatggt gacgatggca atggccgaga gcgcgacgac ttgcaggtag ccgaagggga
     5221 gcgagaagct gccgaggggg atggtgaaga aggtcgcatt gagcgcgccg cccgtgagga
     5281 tgttgaggaa gatggcgaag gcgacggcga cggaggcttg cgagccggtc ttggcgatga
     5341 agatttgcat ccagccatag aggaagccga gccgcccgcc gtaagcctcg cgcacgaaga
     5401 catactcgcc gcccgcgcgc ggcatcatcg cggcgagttc cgcataggtg agcgcgcccg
     5461 cgagcgagag gatgccagcc gcgatccaga cgatgacgac catgtacggc gtgccgacgt
     5521 tgcaggtcat cacgcgcgct ttgaggaaga cgcccgtgcc gatgacgttg ccgatgacga
     5581 tggagacggc ggccagcagg gtcaggccgc gtatgagttt gacgttgtcc gttgctggct
     5641 gtgttgacat gattcaagcg tgcgttgagt aagactgcct tccgacttct ttttacgccc
     5701 ccggcgagat taacttttcc cgccggaggc gcaaaggaga agctcaaggg ttgagtgttt
     5761 aaggtctgtg ctcctgttga ggttgtaccg gcgtattctg aaaagcggcg atgctccatt
     5821 tgccgccctc cttcgtcagg atcatcgtat tgatggcatg acccttttgc gtcgcgccgt
     5881 tcattttaaa ctccagattc cattcgacgt gagccaccgc gacatccggg cgcaggaaac
     5941 gaacgctctt cactgtggcg acgttgcggc tctctttgta gatggtcgag aagatcgccg
     6001 tgtgcccccg ctcgattgtc tctctgcctt tcgcatacat gccgttgacg acaacgtagt
     6061 cggcgtctgt ggcgaaaggc gcggcgaagg ctttgccgtc gtgtgcgttc cagccgtcct
     6121 gcacctgttg gatgatctga cgaacggcgg cttcgtccgt gttcgccgtg gcgggcgtct
     6181 gcgcgttgtg gcgcgccgga aagccgacga agatgagagc cagaattact acggcgaaca
     6241 agtttctgcg ttgagacatc agacaattct ccttttcctg ccggttgagt tgagactacg
     6301 attggtacaa cttacacacg cttcgagaga gcaaattacc gcgccctgat tagccgcgct
     6361 tcgctgcaca tggccgcgca gtggcgcgca tggcggggcg aagttcaagc cctgcgtctg
     6421 gctctcgccg tgccggtgag agccttcgcc gccgcttcgc ccgtcaccac cgcgccttcc
     6481 atgaaaccct gccagtctgc gagatgctcg cctgcgaaga tgactttgcc gtgcggtttc
     6541 tggagtgtgg ggcggacggt gaaccactgg ccgggacgat agacggcgta tgcgccctgc
     6601 gtgtaacggt cgcgctgcca cggcatggat tgaatgctgc gcgcgagttg cgttgcgcgc
     6661 tcgttgaagg gcaggaggtc gcgcgtgatg atctccatgc gacgctgatc ggattgcgcg
     6721 gcgagcacgt cggccttctc gccgacggcg taactgcaca ggatgccctg cgtgccccgt
     6781 tgatcgcgcg tgctgtgaaa gtagtaatga gaagtcacgt cgctgacgag cgaaaaatct
     6841 tccgcgcccc agaagcgttc gtcgaagagt atctggtttt tgacgatgcg cgaatattga
     6901 agctgctcgg cggcggcgct ttgggcggca gggagcggcg ggtcgaactt aatctggtcg
     6961 agcacgcgcg cggggacggt gcagatgcag gcgtctgcct gatagagcac gcctccggct
     7021 ttcactgtga cacgtcctgc gcgttgccga atctcttcga cctgcgcgcg ggtgcggatg
     7081 gaatcgtggc cgatgcgttt cgcgaactct tcgatgatgc gactgttgcc gccgacgatc
     7141 ttgaagtcca tttcgttggc gggactcgat tctaaatact ccgacgcggc ggcataggcc
     7201 gagacgtgac ggatggactc gccgaagtcc gtgctgtcct gcaaatcgcg cagcagcagg
     7261 tcgtcttcag ggaagccgag ctgttcaagc cacgtccacc agtcgagttg gtcgagtctt
     7321 cgacgttcgc gttcggtgta gtggtgatag cgttttttga atagctcgaa gacggcttcg
     7381 gcctgcgcgg agaaacccca tgagccggga ggcgagacgc gcgcatcgcg catgagcgtg
     7441 gcctcgaagc gatgatcctt gagcgggaga gcaaactcgc ggcagagcgc gcgcatccgt
     7501 tcgtggctcg cgccgatcca ttcgccgccg agttcgcaga cgagttcggg attttggtcg
     7561 aaacggaaag aatggacgcg cccgccgtgt cgcgcgcggg cttcgaggat cgtcacgtcc
     7621 caattcgcgc gcttcaaagc gtaagcggcg gcgaggccgg agagtcccgc gccgacgacg
     7681 atgcacgaat gtttgccgcg atggcgctgc gtctgccccg acgaactgtc gagcagcagg
     7741 ggcaggccga gcgcggctgc gccgaaagat ttaagaaacg cgcggcggtt gcttagagtc
     7801 ttttttgtgt tcacgaatcc ttctcgcggg cggggtcgcc ggagtcgtag cttaccagat
     7861 gtccccggtc agtaagtctt cataagtgtc gcggcgacgg actagacgtg cgtggccgcc
     7921 ttctccaatc atcacggcgg cgggcagcgg gcgaccgttg tagggaaacg cctgcgcgag
     7981 cgaatatgcg ccgctgttca ggagcgcgag cacttcgccg gggcgcacct ccgccggaag
     8041 cctgcgataa tcgggcaggc gattttcgcc ttcgaggtcg aaatagacat cgccggaatc
     8101 gcacagaggc cccgcgactt tgtagccggt gtcgggcggc gcgtcggcgc gcgaagccga
     8161 gatgagatgg tagtaccatt tgtaggtcgt catcgagagc agcaggttga agcccgcgtc
     8221 ggtcaggagc cacacgtcgc ccgtctccgg tcgccgcttg atgttgcgca cggtggtcaa
     8281 gaccaacccc gcatcggcga tcacgctacg accgggttcg agcaggatcg tgagacgatc
     8341 cagcaggtgt tcggcctctg tcgcgcgcgc cgactcgcgc gcgacgcgaa gcgcggcgtc
     8401 gagcacctcg gcgggttcga gacttgcgcc gagcatgtcg cgttcggcgg cgggcagcgt
     8461 gtcggcctgc gagcggtcgc gcaggtagtt gacggggatg ccgccgccga ggttgatgtg
     8521 ttcgagcatg tgccccgttt cacgatgaag cgcgacgagg tgctcccaca tcgccttgaa
     8581 ggcggcggcg aagggttcga catcgggcgt ctgcgagccg atgtggatgt gtatgccgcc
     8641 gacgcggata agacggggat gcatcagcgc gcgacgaaag gcagcgggca cttccgtcga
     8701 agtgatgccg aacttcgagg tcagcagggc ggtctgcaaa ccgatgtgcg agcgcgtgcc
     8761 gatctccggc accagacgca gggtgatgtt cgcacgtttg ccgacgcgcc gcgcggcagc
     8821 ttcgacgagt tcgatctcgt agacggaatc cacgttgatc gaataaattt cggcgcgcac
     8881 agcatcgtcg agttcgtcgt ccgtcttgct cgtgccgttg aagatgatct gtgcgggatg
     8941 aaatccggcg cgcagggctt taaagagttc gccgccgctg ttgacttcga tgtcgattcc
     9001 ggcttcgcgc accgccttga gcacggccat gttcgagttg gccttcgagg cgtagcagaa
     9061 tttgaagggg cgacgggtgg aagcggcggc ctgttgcagg cgtgcgatgt tcgagcggat
     9121 gcgcggctcg gagaagacgt agaggggcga accgtattcg cgcacgagtt cgcaggcgtc
     9181 cgcgccgtcg atgtgcagac gcccttcgtg cgcttcgaga tagccgtgga tcgtccacgc
     9241 gggcgcgggc ggcgcgggcg tttgtgctgg catggtataa gactccttga agttcaccct
     9301 cggagagaag acgcgtgcga agatagactg acgccgcaac gcggtcaaga gacgagagga
     9361 aaagggcgac ggggcataag ggtgagaggc ataagagcag aagttgagag gccagcttac
     9421 aagttagcca acggcttctc ctttaccctt tccctctgcc tcttcccctc cattgcggag
     9481 catgaagatg gataaagcgt ttgactggca gcgagtgcgc gaagaatttc cggtgacgga
     9541 gcggctggct tatttgaaca gcgcgggcgc ggggcctgtg ccgcgcgcgg cgtcggacgc
     9601 ggcgttggag ttttatcgtg agatgctgga gtcgggcgac aaacgctggt tcgattggtt
     9661 ggagcgacgc gagcgtgcgc gtgcgacgat ttcccaactg atcaacgccg agccggaaga
     9721 gatcgcgttt acgatcaaca cctcgacggg gatgaacttg atcatcgacg cgctggaagg
     9781 tcgcggcgaa gtcgtctcct gcgagttgga gtttcccgtc tcgacgatca cttggatgca
     9841 caggggcatg cgcgttcatc tgctgaaagc gcggggcggg gaagtgagcg tcgaagacgt
     9901 gtcggcggcg atagcctcaa ggcgcacggg cgtcatctgt ctgagccacg tgcagtattc
     9961 gaacggcttt cgcgcgccgc tcgcggagat cggcgcgcgt aaaggcgagc acgcttttgt
    10021 cgtcaacgcg gcgcagtcgg cgggagtttt tcagattgac gtaaagcgca tgaagatcga
    10081 cgcgctctgc gcgacgggtc acaagtggat gctcggcggc ttcggttcgg gcttcgtcta
    10141 tttaagccgc gcgctgctcg aagcgacgcg cccgcgcgcc gtcggttgga tgagcgaaga
    10201 gcatcccttc gagatgcgca acgacgagtt tcatgtgcgc ggggatgcgg cggcgcgtgc
    10261 ggagatgggt tgcccgcatt tcgcgggcat cttctcgctc ggcgcgtcgg ccaactacat
    10321 gttgcaactc ggaacgggag agatcgagcg tcgcgcgctc gcccttaacc ggatgttgac
    10381 agagcaactc gcggcggctg gctggcgcgt cttgtcgccg ctgcgcgacg aagcgacgcg
    10441 ttcggcggag acgctcgtgg cggcggatga tccggcgcgc gtcgcgcgtc atctctcgac
    10501 gcgcggcgtc gccgtcacgc aaaagcccga aggcatccgc gtcgccacgc acttcttcaa
    10561 caacgaagag gatatcgcgc ggctcgtcgc cgcactcgat gaagttcgtt cgtgaggaac
    10621 gccgggaggc gcggtcttaa gttttaatca caccgaaccg aagagaagga gcagagacga
    10681 tgagccttga gcgaccgcta ttcattccgg tgattttagg cacgccgcga cagggacgca
    10741 tgagcgaaca cgccgcgcgt ttcgttttcg gcgaactgga aaaacgcgcc ggaatagaga
    10801 cggagtggat tgacgtgcgc gagttgaacc tgccgacgac ggatgcgggc gaagcgatca
    10861 aggacgcagg gttcagccgt gcgtgtgctc gcgccgacgc cctcgtgatc gtggtgcccg
    10921 aatacaatca cggttatccg gggctgctca aacacgcgct cgatacgaac ttgaaggagt
    10981 acatacacaa ggccgtgggc gtgtgcggcg tctcggcggg cggcttcggc ggcacgcgcg
    11041 tgatcgaaca cctgctgccc gtcctgcgcg aactcggcct cgtcgcgatc ttcaacgacg
    11101 tgaacttttc cagagtgagt aagattttcg acgagtcggg cgcgctgctc gacgagtctt
    11161 acgtcaagcg cacgaagagt tttctcgacg aactcgtctg gatggcgaag gtcttgcgct
    11221 acgggcgcga aaacgtggtc gtggaataaa cccgtgctcg cgccccttcg cgccgcgaac
    11281 gcacgcggcg cgaggatgcg ccggccacgc ttcgatgcgc ctttaagttg ggacgcaacc
    11341 ttcgggcggc gggcggaatc agaagtgact tgagcaagga gagacgttaa gaccgtttta
    11401 tgagttcagt acatgacgca agcgcgcccg ttgccgccgc tgccacctat ctcaaccccg
    11461 tctacgggcg cgacttccca gaccctttca tcctcaaaca cgcgggagaa tactgggctt
    11521 atgcgacggg cttctggcgc gacgggcgcg ccttcggcgt gctccactcg cgcgacctcg
    11581 ttcattggcg cgaacttgaa ggcgcgctcg caccgctccc cggcgagcac ccctgctact
    11641 gggcgcccga agtgacttac gaaaacgggc gtttcctgat gtactacagc gtcggcaacg
    11701 agaagcggat gcacattcgc gtcgccgtcg cggagcatcc ggcgggaccg ttcgttgaca
    11761 gcgggcacca gttgaccgac gccgagtttg ccattgaccc gcacgtcttc atcgacgttg
    11821 aaggcacgcg ctggctcttc tacgccacag actttctcac gcacacgcac atcggcacgg
    11881 gcaccgtgcg cgaacgaatg ctcgacccgt tcacgctcgc gggcgatacg cgccccgtga
    11941 cgcgcgcacg ctacgattgg caggtctacg accccgcgcg ccaggagaag ggcggcgtgc
    12001 gctggcacac cgtcgaagga ccctttgtgc tgcggcgcaa aaatctctac taccagatgt
    12061 tcagcggcgg caactggcag aatttgacct acggcgtgag ctacgccgtg agcgaccgca
    12121 tcgaacgcga agacgaatgg gagcaggccg ccgacggcgc acgcgtgctg cccatcctgc
    12181 gcacgatccc tgagcgcgtc atcggtcccg gccacaactc ggtcgtgcgc gggacggaca
    12241 atcgagagtt gttctgcgtc tatcatcgct gggacggcga acacgggcgc gtgctcgcca
    12301 ttgaccggct cgattgggtg ggcgaacgca tgaccgtgct cggcgcgagc acgacgccgc
    12361 agcccgcgcc cgcccccccg accttcgcag gcttctacgg cgaacatgag gacgggttgg
    12421 gcgaaggctg gacgtgcacg acgcagggtg gcgcgcagtg gagcgtgcgc gagggcgaag
    12481 cgcggcagga gacggtggcg gagggagcgc gtgcgtctgc gcgttgcgat gcgcgcgcgg
    12541 cgagttttat tctggaagta agtttgcgcg cgctcgatgc ggaggcacag aggggcgcgt
    12601 tcggcgtcgc gctgttgagg gatgatgaag agagcgcgct ccggtttttg cttgagccgg
    12661 agacgggaga ggcgcgtgtg gactggcagg cggaagacgg cacgcgcgct gacgtgcgct
    12721 ttgcgctgcc ttccgatttc gcgccgcgtg cctttcacct gttgcgagtt gtcgtcgata
    12781 acgcgtgtgt gactatttcc cttgacgaaa gagttgtgcg ctggcaaggt actttaaaag
    12841 ccgcgccgga ctcggctgcg ctcgtcacca cagagggcgc aagggcggca ttcaagggct
    12901 tcgaactaac acacggctgg gaagaactgt tcacgcagac ggagcggacg ctcgccgcgc
    12961 gcggctggca acacaactcc gaaggctggc atgtgcagga tgatctgttg cgcttcgacg
    13021 accgggaagg tcgtccgaac gccgctatcg tcaaagggac gctgcttgag tcttacgagt
    13081 tggtcgtcaa cgtgcggctt gagcctgagc ctgcgcccgg caatgggtgt tacggtttct
    13141 acccggctta tgtcgtgggc gcagcagagc aggagcagtt aatcacggtc gagcgagagg
    13201 gcggggcaga gggcgaatgt gcgctcgtcc tgcgcagttt gagagatgat tcgaacaggg
    13261 aaagctggcc gctccccgca tcgttcgacc cgtctgtgat gcagcagttt cgctttcgca
    13321 aacagggcgg gcgtctggcg atatggtggg aagccgcgcc gctcggcgaa atcagcacaa
    13381 ccacagaggc caccgccatc ggcctctacg cacaacgcgc ccgcgtcgcc tgcgacatgg
    13441 tgcgcgtcac ggctctgaga acagtgacga gtgacaagtg acgcgcgaca agttgaaaaa
    13501 caaaacttgc cccgaccaca cttgctttat ctcgtcactt gtcacttgtc acttgtcacc
    13561 gtcttcttat gactgaattg atactgggaa cgaggccgga agatttcatc tgggcgagcg
    13621 gcgtagagga ttcgtttgtg ccacaggtga aggcggggca tcgcgcgctc gacgaatacc
    13681 agttgatggg acattacgag cactggcgcg aagacctcgc gctcgggcgc gaactgggcg
    13741 tgcgcgcttt gcgctggggc gtgccctggt atcgtgtcga gccggagccg ggcgtcttcg
    13801 attggcgttg gacggacgaa gtgctgccct acatgatcga agaactcggc atcacgccca
    13861 tcgtcgattt gatgcactac ggctgcccgc tgtggttgaa gaaagagttc atcagtaaag
    13921 attacgtccg cgccgtctcg gcttacgccg ccgccttcgc gcgccgctac aaagattccg
    13981 tcaagtggta cacgcctctc aacgagccgc tcgtgaacgc cctgatgtgc ggcaagcgcg
    14041 ggttgtggcc gccatatctt cgcggcgacg tgggctacat ccgcatcatg ctgcaactcg
    14101 tgcgcggcat catcgccacc gtcgaagcga tcaaagaggt tgacccgctc tcgatcatgg
    14161 tgcacgtcga agcgaccgga ctcagccgcg ccatccggca ggatttggaa tcgctcgcgg
    14221 cggaagagca gcggcgcggt tatctcgcct acgatcttct cgtcggcagc atcacgcacg
    14281 atcacccgct cttcccgtgg ctcctgcgca gcggcgcgag cgtggacatg ctcgacgaga
    14341 tcgcgcgcca caaaatctcg ctcgacgtgc tcggcatgaa tttctacccg cagtggtcaa
    14401 cgcagcatct ctacgtcgac cccaaagggc gtctcgccta tcgcgcgcac gaggaggacg
    14461 gcgtcggctt cgcagccgtc atcaacgatt actatcaacg ctacaaagcg cccatcatca
    14521 tcacggagac gagcgcgttc ggttcggagg aactgcgctc gcgctggctc aagtcatccg
    14581 tcgaggccgt caaatactta cgcagccagc gcatccccgt cgtgggctac acctggttcc
    14641 cgatgttcac catgatcgat tggcgttacc gtcacagccg cgcgccactc gaacacttcc
    14701 atctcgaact cggcctctac cggctgaaca gcaaaggcga cgcgtccacc ccggcgcagg
    14761 ggcgttggag cgcaaccccg ctcgttgaac aggtgcgcgc ctacatcaca gactccgtgt
    14821 cctcggtcgg cgcgctcgac ccccgcgcgc tcgcgcccgc cctcaacacc gaagcctgca
    14881 aagcgcacgc acgcacgccg acgccccctg acgccggtac gcccgcgccg gaacagatgc
    14941 cgcagggtta gatcgagaac gattagagcg aatggctctc gccgtcgcag ggggcaagcg
    15001 cgcatctctc ccgcctgcgc cgga
//
LOCUS       DASWNS010000114         4525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_186916, whole genome shotgun sequence.
ACCESSION   DASWNS010000114 DASWNS010000000
VERSION     DASWNS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186916"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VGB76_03910"
     CDS             complement(<1..867)
                     /locus_tag="VGB76_03910"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0098082.1"
                     /translation="MSFRSKFLIVVFSATLALYAVVGGLMYTRAQQQNDPGAQLRIFE
                     SVLQHIHDDYVDEPDLNKVRAGALRGLAYGLDPYSTYLTVDQVRDFRVGLDKERAGIG
                     AELSQVASYLYVIAPVKGGPADLAGVQAGDIIEYIDTKATRDISLYDAKQLLNGASGT
                     EVKVRILRAGTRPLTLTIKRAPVQAPGVETRVEASKVGVVRINSLMEGEAAIVQKRVQ
                     ELQKQGSQKLVLDLRGVAGGSLAEGVKVANLFIKDGALAQTIGRGNKSLKTFTADPKL
                     AIFEGPVAVLIDR"
     gene            1046..1528
                     /gene="ruvX"
                     /locus_tag="VGB76_03915"
     CDS             1046..1528
                     /gene="ruvX"
                     /locus_tag="VGB76_03915"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY0098083.1"
                     /translation="MPNSDFPSSQNESQPDAAKGRLLALDLGTKRVGVAVSDELQLTV
                     QPLPPLRRSNWKTLLRQISELRHTFDAQGVVIGLPLNLDGTEGASAQDARRIARNLSL
                     SLTVPVHLQDERLTSLAAEEYLRAAGVRGRALSARTDSEAAAIILRDFISERRPPTIG
                     "
     gene            1647..2726
                     /gene="mltG"
                     /locus_tag="VGB76_03920"
     CDS             1647..2726
                     /gene="mltG"
                     /locus_tag="VGB76_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY0098084.1"
                     /translation="MKIYRLLAILVLLLACAAAVFSYWFYRELNKPVLHSHSTQYIEI
                     PRGSTPDQTIARLQSSGVIGRSWPLRFYIRFRGLGPRLKAGEYRFPSPISPLGVLRKL
                     EEGQQRLARFTVIEGWTRWDIASAMARLPELKLANADEALALMDDTSEIRDLDAQATN
                     LEGYLYPDTYSFPPETSAREMIAAMVRRFRQVWRDQVGATEAITSKRTPREIVTVASL
                     VETEAKLKDERPLVASVIYNRLKIGMPLGIDSTVIYASKLAGKWRNDGKVYQSDLDRD
                     SPYNTRKVRGLPPGPIASSGARSLEAALRPAESDYLYYVREPSRDDGAHNFYNNEADF
                     YRGVQALRAWERARDANAAGANTNE"
     gene            2878..3522
                     /locus_tag="VGB76_03925"
     CDS             2878..3522
                     /locus_tag="VGB76_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098085.1"
                     /translation="MKKVLTLSMFLLLVACNISAQEKGVDRQNDRIRDAGTDRAPGVN
                     GVKQNTGSGRGIDFGKGRTPQATPIPNPYRLSIRRDIVVRAVEELLAERKMIVDTTVS
                     KKDEGVLISQPYRFVKGAVVAGTELSHYAELTQENSRGWTQGRYTFIIEVQPLDGVTT
                     SVAVNARVEGRSDGASGAEWVSLRSSGQAEQEFIIALVEKLTGAPPPAPNGQAQ"
     gene            3620..4261
                     /locus_tag="VGB76_03930"
     CDS             3620..4261
                     /locus_tag="VGB76_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098086.1"
                     /translation="MASNQSINEPANEPHLFEGGETAPSKWIKFGALACALAVAAALL
                     IGYRLLRARQLESVRAAQQAAQVTKVVAPPEAQIFEDEARLKGSDAVITGTIRNISDK
                     RLEDLSVELELKRRTGQATERRQIDVAPASLDPGAEGRYSLNLRSSEWGGARVLSLNS
                     AKRSETIAFKSAVGARRQPERLPQGNPKIVVVPRPRPTGEEFINTPDNPTRIP"
BASE COUNT         1007 a         1340 c         1255 g          923 t
ORIGIN      
        1 gcgatcaatc aagaccgcca cgggaccctc gaaaatggcg agtttggggt cggcggtgaa
       61 agttttgagg ctcttgtttc cgcgcccgat ggtttgcgcg agcgcgccgt ctttaatgaa
      121 caggttcgcc accttgacgc cttccgcgag cgaaccgccc gcgactccgc gcaggtcgag
      181 cactaatttc tgcgagcctt gcttctgcaa ttcctgcacg cgcttctgca cgatggccgc
      241 ctcgccctcc atcaggctat tgatgcgcac taccccgacc ttcgacgctt ccacacgcgt
      301 ctcgacgccc ggagcctgca cgggagcacg cttgatcgtc agagtgagcg ggcgcgtccc
      361 cgcacgcagg atacgaactt tcacttctgt gcccgaagcg ccgttcagca gttgcttggc
      421 atcatagagc gaaatatcgc gcgtcgcttt ggtgtcgata tactcgatga tgtcgcccgc
      481 ctgaacgccc gccaagtctg cggggcctcc cttcacaggc gcaatcacgt acaggtaaga
      541 ggccacctgt gaaagctccg cgccgattcc cgcccgctcc ttatcgagtc cgacgcggaa
      601 atcacgcacc tgatcaaccg tcaggtaagt tgagtacggg tcgaggccgt aggcgaggcc
      661 gcgcaaggcg cccgcgcgca ccttgttcaa atccggctcg tcaacataat cgtcatgaat
      721 atgctggagt acgctttcaa aaattcgcaa ctgcgcgccg gggtcgttct gttgttgcgc
      781 gcgcgtgtac atcaaaccgc ccaccacggc atagagcgcg agcgtggcgg aaaaaactac
      841 tatgagaaac ttactgcgaa atgacatctt tcacctcaag aaaacttttg cgccggattg
      901 tgccaacacc tgtcgagcct ctctgccctc caggctggaa tggatgatac agtagccttc
      961 tgacgcgtcg caagacagtt tggcacgttg gcctgctgcc gtgcgcctgc gctatactcc
     1021 agacgtttga accaaaagat caaatttgcc gaattcagat tttcccagca gccaaaatga
     1081 gtcgcagccg gacgctgcga agggtcgcct gctcgcgctc gatttgggaa cgaagcgtgt
     1141 cggcgtcgcc gtcagcgacg aactacagct aaccgtgcaa ccgctgccgc cgctcagacg
     1201 ctctaactgg aaaactctgc tcaggcaaat ctcagaattg cgacacacct ttgacgcaca
     1261 aggagtggtg attggtttgc cgctcaatct ggacgggaca gaaggcgcgt cggcgcagga
     1321 tgcgcgtcgt atcgcgcgca atctctccct ttcgctcacg gttccggtgc atttgcagga
     1381 cgagcggctc acttcgctgg cagccgaaga gtatttgcgc gcagcgggcg tcagaggccg
     1441 ggcactgagc gcgcgcaccg atagcgaggc cgccgcaatt attctcaggg atttcatctc
     1501 cgaaaggcgg cctccgacga ttggctagac cggctcgaaa aagagtcgtg acgagcaaaa
     1561 acccggcgtt tccaagaatc ggagacaata aaaatgcaat tgctacttat tcaacaatta
     1621 cgccgcctta gttaatctaa tgtctcatga aaatttatcg ccttttagct attttagttc
     1681 tccttcttgc ctgcgctgcg gcggtttttt cctattggtt ttaccgcgaa ctaaacaagc
     1741 ccgtcctgca ttctcactcg acgcaatata tagaaatccc acgcggctcg acgcctgacc
     1801 aaacgatagc tcgccttcaa tcctccggcg ttatcggacg ctcgtggccg ttgcgcttct
     1861 atatacgttt tagaggactc ggcccacgtc tgaaggcggg cgagtatcgt ttcccttctc
     1921 ccatctcgcc gctcggagtg ttgcgaaaat tagaggaggg gcagcagcgg ctcgcgcgct
     1981 tcacagtgat cgagggctgg acgcgctggg acattgccag cgcgatggcg cgtctccccg
     2041 aactcaaact cgccaacgcg gacgaagcac tggccttaat ggacgatacg agcgagatac
     2101 gcgacctgga cgcgcaggca accaacctcg aaggctatct ctacccggac acttacagtt
     2161 tccccccgga gacgtctgcg cgcgagatga tcgccgcgat ggtcaggcgt ttccggcagg
     2221 tgtggcgcga tcaggttgga gcaacggaag ccataacgtc gaagcgtacg ccgagagaga
     2281 tcgtcacggt cgcatcgctc gtcgagacgg aagccaagtt gaaggacgaa aggcctctgg
     2341 tcgcatcagt catctacaat cgccttaaaa tcggcatgcc gctcggcatt gattcgaccg
     2401 tcatctacgc ttccaaactc gccggaaaat ggagaaacga cgggaaagtt tatcaaagcg
     2461 acctcgaccg cgattcgcct tacaatacgc gcaaggtgcg cggcctgccg ccggggccta
     2521 tcgcctcctc cggtgcgcgc tctctggaag ccgcactgcg ccccgccgag tccgactatc
     2581 tctactacgt gcgcgagcct tcacgcgacg acggcgcgca caatttttac aacaacgaag
     2641 ctgacttcta cagaggagtg caggcgctac gcgcttggga acgtgcgcgc gacgccaacg
     2701 cggcaggcgc aaacacgaac gagtaaacgc ttgagagcat aattttcagc gaattcattt
     2761 catccgcccc aactctttct tgaaaagacc tgaacgttgt tttattctcc atacatctcc
     2821 cgtcgtcttg aaattcttcg gacagaggct ctcgtgggcg aggccgaaag gtttgcaatg
     2881 aagaaagtct taacgctctc gatgttttta cttctggtcg cctgtaacat ctctgcacaa
     2941 gagaaaggcg tggaccggca gaacgaccgc atacgcgatg ccgggactga ccgcgccccc
     3001 ggcgtcaacg gcgtcaagca aaatacaggc agcgggcgcg gcatcgactt tggtaaaggc
     3061 cggacacctc aggccacgcc gattcccaat ccttatcgcc tctcgatccg gcgcgacatc
     3121 gtcgtccgcg ccgtcgaaga actgctggcg gagcggaaga tgatcgtcga tacgaccgtc
     3181 tccaaaaaag acgagggtgt tctcatcagc cagccctaca ggtttgttaa aggtgcggtt
     3241 gtggccggaa ccgagttgag ccattacgct gaactgacac aggaaaattc gagaggttgg
     3301 acgcagggac gctacacctt catcatcgaa gtgcagccgc tcgacggcgt gacgacgagc
     3361 gtagcggtca atgcacgcgt agagggtcgc agcgacggcg catccggcgc ggagtgggta
     3421 agtttgcgca gttcgggtca ggcggaacaa gagtttatta tcgcactcgt cgaaaaactg
     3481 accggcgcgc cgccccccgc cccgaacgga caggcgcagt aatcatccgg cgaccgcttg
     3541 cacgtcaggc tccgctaaca tttacaacca tttagccttc cccgtttaaa gttgccagtg
     3601 aaggatttga tttgaagcga tggcgagtaa tcaaagcatt aacgagccgg ctaacgagcc
     3661 gcatttgttt gaaggagggg agactgcccc ttcgaagtgg attaaattcg gggcgctcgc
     3721 ttgcgcgctg gccgtcgccg ccgcattgtt gatcggttac cgtctcttgc gtgcacgcca
     3781 gcttgagagc gtgcgcgcgg ctcaacaggc tgcgcaggtt acgaaagtcg tcgcgccgcc
     3841 cgaagcgcaa attttcgaag acgaggcgcg gctcaaaggc tccgacgcgg tcatcacggg
     3901 cacgatccgc aacatctcgg ataaaagact cgaagacttg agcgtcgagt tggaattgaa
     3961 gcgtcgcacg ggtcaggcta ccgagcgacg tcaaatcgac gtcgcgcccg ccagtctcga
     4021 tccgggcgca gaaggtcgct actctctgaa cttgcgctcc tccgagtggg gcggggcgcg
     4081 cgtgttgagc ctcaacagcg cgaagcgctc cgagacgata gctttcaagt cggcagtggg
     4141 agcacgccgc cagcccgaga ggcttcctca aggcaatccc aagatcgtcg tcgttccgcg
     4201 tccgaggcca acaggcgagg agttcatcaa cacgcccgat aatccgacgc gcatcccgta
     4261 gccgcccttc ttcaagtaat acttgacaag tcggcgcgac gaccaatttc aggtaaaacc
     4321 ttaaggtctg tgtttcttga ggatttttta gttatttatc tcgcaggact ttgcgaacag
     4381 acaaccggcg gatgtttttc tccgtcacaa caaagtccgg tgcaggttca acgcgaaaat
     4441 cgtttctccg taaagaatga acaaccaggc cacctgcgat aattcgtgcg aaaactatcg
     4501 acgccaaaat tgatgtcatt aagat
//
LOCUS       DASWNS010000115         5365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_30861, whole genome shotgun sequence.
ACCESSION   DASWNS010000115 DASWNS010000000
VERSION     DASWNS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30861"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2023)
                     /locus_tag="VGB76_03935"
     CDS             complement(<1..2023)
                     /locus_tag="VGB76_03935"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0098087.1"
                     /translation="MRRKFFQLLLVVVLAASALAGQERQSRSASAPEQKRLREHIEYL
                     ASADLEGRRTGTKGATVAAAYIAKEFERYGLRGGGYSAALRSKERAASYTQSFPYVSG
                     VELGKGNAMTFAPRASGTAEAHAAPASLDLRLGEDWMPLAWSANARVESSPVTYVGYG
                     ITAAELGHDDYAGVDLKGRLALAFAGAPDGDNPHGSFARFADLRFKAAAARDHGARAL
                     LVIAREENFKDDKLARLRVDQSVASGDAGLPVVVISRQAARRALESAATPAVRFEELE
                     NALLQTAPKTAPRDGVESAQGATHASKRNFSAPLRNVEFAVATEIVRRESPAVNVIGI
                     LEGSDPNLKNESIVIGAHYDHLGRGGQGSLAAREGEIHHGADDNASGVAGLLELARTF
                     AKDRESIRRTIVFIAFSGEEEGLLGSSYYVRNPVVPLTQTVAMINLDMIGRMKENRLN
                     IGGVGTSSEWKNWIEKANHALQLNVTAAAGKSSPEEELKRGNYPVVTAANGGASANAA
                     PGEHFALTLTEDGFGPSDHASFYAKQTPVLFFWTGTHEDYHKPTDTADRINYEGEQRI
                     VRFVSEIVRVLDANDKRPAYTLAKSDARAGRSPGFRVSLGTIPNYAETTDGMKLDGVS
                     EASPAAKAGLRAGDVIVRLAGRDVRNVYDYTYVLGEMKAGVEYDVEVMRA"
     gene            complement(2100..3188)
                     /locus_tag="VGB76_03940"
     CDS             complement(2100..3188)
                     /locus_tag="VGB76_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098088.1"
                     /translation="MKKLFPLALLVSVCAMLAATFSTAQTTTTQSAPGSKNEGVALAS
                     ERHLRNVRQLTFGGENAEAYFSGDGKQLIFQSKRDGRGCDQIYMMRADGSDVRMVSNG
                     TGRTTCSYIFPKNPRRILYASTHLGARECPPNPDFSKGYVWAIYPSYDIFTANADGSN
                     VKRLTDAEGYDAEATISPDGKKIVFTTMRSGDLDIYTMDTDGRNVRRLTTELGYDGGA
                     FFSTDSKQIVYRSYHPQTPEQVARYKQKLAENLIEPNTFEVWVMNADGSNKRQVTKLG
                     AASFAPYFFPDAKRIIFASNVNDPKRRNFDLYSIKVDGTGLERITYEETFDGFPMFTP
                     DGKKLVFASNRNAKTRGDTNIFIADWVE"
     gene            complement(3239..4324)
                     /gene="serC"
                     /locus_tag="VGB76_03945"
     CDS             complement(3239..4324)
                     /gene="serC"
                     /locus_tag="VGB76_03945"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY0098089.1"
                     /translation="MTERIYNFSAGPAVLPVPVLEEAQRDMLSLPGVGMSVMEISHRS
                     KTFDEIIHGAESSLRELLGIPDHYKVLFLQGGASLQFSMIPINLLAEGQTADYIITGS
                     WGKKALKEAKRVGTTSVVADTADSRYSRTPEQHELKLSQDAAYVHLTSNETIEGVEWK
                     QLPDVGNVPLISDASSNILSRPEPVERYALIYAGAQKNMGPSGLTLVIIREDLLARAP
                     SGLHTMLDYRTHVENGSLYNTPNTWGIYILNLVCKWLKGKGGLEGMERENEAKAALLY
                     DAIDATDFYRGHAAPEARSLMNVTFRLPTEDLEKQFVQEATAAKLDGLKGHRSVGGLR
                     ASIYNAFPREGVEALVEFMKEFERKNG"
     gene            complement(4360..5148)
                     /locus_tag="VGB76_03950"
     CDS             complement(4360..5148)
                     /locus_tag="VGB76_03950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0098090.1"
                     /translation="MKTTLITGASSGIGEVFARRLAGRGENVVLVARSEEKLNALCNE
                     LGRAAAGVNCQTIVSDLSEAGAARRLFEETERRGLEVDTLINNAGFGSMGDFLSLDVG
                     RELNMIDLNVKSLVELTYLYLAPMRRRRRGAIINVASTAAFQPVPYMATYAATKAFVL
                     SFTEALWDENRHYNIRVMALCPGATETNFFDAAGVKKPPFRVAQTAEQVVDTALHGLD
                     RGKGHIISGWINYVQTQAERLVPRSLVTRIAGKGMRSGLNIERD"
     gene            complement(5156..>5365)
                     /locus_tag="VGB76_03955"
     CDS             complement(5156..>5365)
                     /locus_tag="VGB76_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098091.1"
                     /translation="TLEEHYVATAKLSATEHFPKLGLTDAGILNLAKDQYLVLSNDFK
                     LVGYLSKQGVDAINFNYIRTLNWKL"
BASE COUNT          967 a         1641 c         1616 g         1141 t
ORIGIN      
        1 ccgcgcgcat gacctccacg tcatactcca ccccggcctt catttctccg agcacgtaag
       61 tgtaatcgta gacattgcgc acgtcgcgcc cggcgagacg cacgatcacg tcgcccgcgc
      121 gcaagcccgc cttcgccgcc ggtgaagctt cgcttacgcc gtcgagtttc atgccgtcgg
      181 tcgtctccgc gtaattcggg atcgtgccga gagagacgcg aaagccgggg gagcgtcccg
      241 ccctcgcgtc cgacttggcg agcgtgtaag ccgggcgttt atcgttcgca tccagaacgc
      301 gcacgatctc cgagacgaag cgcacgatcc gctgttcgcc ttcgtagttg atgcggtcgg
      361 ctgtgtcggt gggcttgtga taatcctcgt gcgtgcccgt ccagaagaag agcacgggcg
      421 tctgcttcgc gtagaacgaa gcgtggtcgc tcggaccgaa accgtcttcc gtgagggtca
      481 gcgcgaaatg ctctccgggc gcggcgtttg cgcttgcgcc cccgttcgcg gcggtgacga
      541 cagggtagtt gccgcgcttc aactcttcct ccggcgaact cttgcccgcc gccgctgtga
      601 cattgagttg gagcgcgtgg ttggcttttt cgatccagtt cttccactcg gacgacgtgc
      661 cgacgccgcc gatgttcagc ctgttctctt tcatccgccc gatcatgtcg agattgatca
      721 tggcgacggt ttgcgtgagc ggcacgacgg gatttctcac gtagtaactc gaaccgagca
      781 agccctcctc ttcgccgctg aaagcgatga agacaatcgt gcggcgaatt gattcgcggt
      841 ctttcgcaaa cgtgcgcgcg agttcgagca gaccggcgac gcccgaagcg ttgtcgtccg
      901 cgccatgatg aatctcgcct tcgcgtgcgg cgaggctgcc ctgcccgccg cgccccaggt
      961 gatcgtagtg tgcgccgatc acgattgact cgtttttcaa gttcggatcg gaaccttcca
     1021 gtatgccgat gacgttgacg gcgggggatt cgcgacgcac gatctcggtc gccacggcga
     1081 actcgacgtt gcggagcggc gcggagaaat tccgcttcga cgcgtgcgtc gctccctgcg
     1141 cactttcgac gccgtcgcgc ggcgcggtct tgggcgcagt ttggagcagc gcgttttcca
     1201 gttcctcaaa tctcaccgca ggcgtcgccg ccgattcgag cgcgcggcgc gcggcctgcc
     1261 ttgagatgac gacgacggga agccccgcat cgcccgaagc gacgctttgg tccacgcgca
     1321 ggcgcgcgag tttatcgtcc ttgaaattct cttcgcgcgc aatgacgagg agcgcacgcg
     1381 cgccgtgatc gcgcgccgcc gccgctttga aacgaaggtc tgcgaagcgc gcaaaactgc
     1441 cgtgcgggtt gtcgccgtcg ggcgcgcccg cgaaggcgag cgcgaggcgt cccttcaagt
     1501 ccacgcccgc gtagtcgtcg tgccccaact cagccgccgt gatgccgtag ccgacataag
     1561 tgacgggcga actctccaca cgcgcgttcg cgctccaggc gagcggcatc caatcctcgc
     1621 ccagacgcag atcgagcgac gccggagcag cgtgtgcctc cgccgtcccg ctcgcgcgtg
     1681 gcgcaaacgt catcgcgttg cccttgccga gttcgacgcc ggagacgtaa gggaaactct
     1741 gcgtgtagct ggccgcacgc tctttgctgc gcaacgctgc gctgtaaccg ccgccacgca
     1801 gaccgtaacg ctcaaactct ttcgcgatat acgccgccgc caccgtcgcg cccttcgttc
     1861 ccgtccggcg gccttccaga tcagccgagg cgagatactc tatgtgctca cgcagcctct
     1921 tctgctccgg cgcgctcgcg ctccgcgact gccgctcctg ccccgccagc gcgctcgccg
     1981 ccagcacgac gacgagcaac agttgaaaaa atttacgtct cataaataac ccggaggtcg
     2041 gagatttgta gaagatagtt ttgactgatc tctaaaccta cggcctctga tctctgctgt
     2101 tattccaccc aatcggcgat aaagatgttc gtgtcgccgc gcgtcttggc gttgcggttc
     2161 gaggcgaaga ccagtttttt gccgtcgggc gtaaacatcg ggaagccgtc gaaggtttcc
     2221 tcgtaggtga tgcgttcgag tcctgtgccg tccaccttga tcgagtagag atcgaagttg
     2281 cgacgcttgg gatcgttgac gttcgaggcg aagatgatgc gcttggcatc ggggaagaag
     2341 tagggggcga aactggccgc gccgagtttc gtcacctgcc gcttgttcga gccgtcggcg
     2401 ttcatcaccc agacttcgaa cgtgttcggc tcgatcaaat tctccgcgag cttctgcttg
     2461 tagcgcgcca cctgttcggg cgtctgcggg tgataggagc gatagacgat ctgcttgctg
     2521 tcggtggaga agaacgcgcc gccgtcgtag ccgagttcgg tcgtcaggcg gcggacgttg
     2581 cgcccgtccg tgtccatcgt gtagatgtcg aggtcgccgc tgcgcatcgt ggtgaagacg
     2641 atcttcttgc cgtccggcga gatggtcgct tcggcgtcgt acccttcggc gtcggtcaga
     2701 cgcttgacgt tcgagccgtc ggcgttcgcc gtgaagatgt catacgaggg atagatcgcc
     2761 cagacgtagc ctttcgagaa atcgggattg ggcgggcatt cgcgcgcgcc gagatgcgtg
     2821 gaggcgtaaa ggatgcgccg gggatttttc gggaagatgt aggaacaggt cgtgcgtccg
     2881 gtgccgttcg aaaccatgcg cacgtcggag ccgtcggcgc gcatcatgta aatctgatcg
     2941 cacccgcgcc cgtcgcgctt cgactgaaag atgagttgtt tgccgtcgcc gctgaaatag
     3001 gcttcggcgt tctcaccgcc gaacgtcaac tggcgcacgt tccgcaggtg acgttccgac
     3061 gccagcgcga cgccttcgtt tttactaccc ggcgcgcttt gtgtggtggt tgtttgcgcc
     3121 gtcgagaatg tcgcggcgag catggcacag acggagacga gaagtgccag agggaaaagt
     3181 ttcttcatac cttttaaaaa ccaaccgtac ttttaaaaac cagccgtact ttcaaaaact
     3241 agccgttctt gcgttcgaac tctttcataa actcgaccaa cgcttcgacg ccctcgcgcg
     3301 ggaaagcgtt gtagatcgag gcgcgcaggc cgcccaccga gcgatggcct ttgaggccgt
     3361 cgagtttcgc ggcggtcgct tcctgcacaa actgcttctc caaatcttcc gtgggcaggc
     3421 ggaacgtaac gttcatcaac gaacgcgctt cgggcgcagc gtggccgcga taaaagtctg
     3481 tcgcgtcaat ggcgtcgtag agcagcgcgg ccttcgcttc gttctcgcgc tccatgcctt
     3541 cgaggccgcc tttgcccttg agccacttgc agacgagatt caggatgtag atgccccacg
     3601 tgttcggcgt gttgtagagc gacccgtttt cgacgtgggt gcgatagtcg agcatcgtgt
     3661 ggaggccgga gggcgcgcgc gcgagcaggt cttcgcggat aatgacgagc gttaagcccg
     3721 atggtcccat gttcttttgc gcgcccgcgt agatgagcgc gtaacgctcg acgggttcgg
     3781 ggcgcgagag gatgtttgac gaagcatccg agatgagcgg cacgttgccc acgtccggca
     3841 gttgcttcca ttcgacgcct tcaatcgtct cgttcgaagt gagatggacg taggccgcgt
     3901 cctgagagag tttcaattcg tgctgttcgg gcgtgcgcga gtagcggctg tcggcggtgt
     3961 cggcgacgac gctcgtggtg cccacgcgct tcgcctcttt gagagccttc ttgccccaac
     4021 tgcccgtgat gatgtagtca gccgtctgac cttcggccag caggttgatc gggatcatcg
     4081 aaaactgcaa actcgcgccg ccctgtagaa agagcacctt gtagtggtcg ggaatcccta
     4141 acaactcgcg cagagagctt tccgcgccgt gaatgatctc gtcgaaagtt ttcgagcgat
     4201 ggctaatctc catcacggac atcccgacgc cgggcagaga caacatatcg cgctgcgctt
     4261 cttcgagcac aggcacgggc aggacggctg gacccgcgct aaaattatag attctctcag
     4321 tcatcttttc tcttcctgat ctcttttcct gaaatgagtt tagtctcgct cgatgtttaa
     4381 acccgaacgc atgcctttac ccgcgatgcg cgtgacgagg gagcgcggca cgaggcgttc
     4441 ggcctgcgtc tgcacgtaat tgatccagcc ggagatgata tggcctttgc ccctgtcgag
     4501 tccatgcagg gcggtatcca cgacctgctc ggctgtctgc gccacgcgaa acggcggctt
     4561 cttgacgccc gccgcgtcga agaaattcgt ctccgtcgcg cccggacaga gagccatcac
     4621 gcggatgttg tagtgccggt tctcgtccca caaggcttcg gtgaaagaga ggacgaaggc
     4681 tttggtcgcc gcgtaggtcg ccatgtaggg cacgggttgg aaggcggcgg tcgaggcgac
     4741 gttgatgatc gcgccgcgcc tgcgcctgcg catcggcgcg agatagagat aggtgagttc
     4801 gacgagcgac ttgacgttca ggtcgatcat gttcaactcg cgccccacgt cgagcgataa
     4861 aaaatcgccc atcgaaccga agcccgcgtt attgatgagc gtgtcaactt ccaatccacg
     4921 ccgctccgtc tcctcgaaca ggcgacgcgc cgcgcccgct tctgataagt ccgacacgat
     4981 tgtctggcag ttcacgcccg ccgccgcccg ccccaactcg ttgcacagcg cgttcaactt
     5041 ctcctccgaa cgcgccacca gcacgacgtt ctcgccgcgt cccgcgagcc gccgcgcgaa
     5101 gacctcgcca atcccgctcg acgcgcccgt gatgagagtt gttttcattc tctgcctaaa
     5161 gcttccaatt caaggtgcga atataattga aattaatcgc atcaacgcct tgtttgctta
     5221 aataaccgac caatttaaag tcgttagaca agacaagata ttgatctttg gctaaattta
     5281 agattccagc gtctgtcaga cctaatttag gaaagtgctc ggtggcagac aactttgcgg
     5341 tcgcaacgta atgttcttct aaagt
//
LOCUS       DASWNS010000116         6216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_56495, whole genome shotgun sequence.
ACCESSION   DASWNS010000116 DASWNS010000000
VERSION     DASWNS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56495"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..3762
                     /locus_tag="VGB76_03960"
     CDS             16..3762
                     /locus_tag="VGB76_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0098092.1"
                     /translation="MRKRRLFIVVIVLTGGVFMSALLSHRASNRVAVAQESRLPAAAR
                     EAAYRANNLGVALLEQFKHREGAEQFERALKLDPQLALARINLAIALYNVPDAEAAAR
                     EAASALTLAPRAPQALYILALVARSRNNTAEAVEAFRRVLQVDAQDTGANVNLGQLYA
                     QGRNYAEAERAFRAALASEPYNTTALYGLANVLLRSNQREEGQRRLQEFQVLRQSGAG
                     TSLGTNYLEQGRYAEAVTSTGAEAGLVDPRTPEVVYTDATEGVLPVAPAPATRTPPSA
                     NARRTRPAAPEQYDVSLRSPATRGVATLFDFDVDGDLDIFDSASGGQRLYRNDGGRFV
                     DVTANSGALASRVAGVGLAAVAGDYDNDAKPDLFVLYEGRVALYHNEGAGKFSDATLA
                     AGIASATHHSISAAFADVDHDGDLDIFTGGHSDLSKASVSTQATAKAGELKLDAPPAP
                     NRLLRNNGDGKFTDVTAGAKVAGERSHTVAVVPTDYDNRRDVDLLVVNYEGAPALYRN
                     LRDGSFRDVAREVGLELKGRYLSAAAGDVNKDGFTDFFFGAEASAGTFALSDGQGRFN
                     LKPAPNATRDASAAQFLDYDNDGLLDLLIDASGGMRVWRNLGNDWSDVSERAVGQTLR
                     GDGAREKAGESRVLASGDLDVDGDTDLIVRDTTGRLRFARNDGGNRNRSLRVRLAGRV
                     SNRSGVEAKVEMRAGSLWQKLEVYAASPAPAPADVVFGLGQREAADAVRVIWPSGTVQ
                     AETEFSPAATVAGASNEKGSDPRSATKDAQRTTAAATSNATLTVTELDRKPSSCPYLY
                     TWNGERFEFITDFMGGGELGYWLAPGVRSAPDPDEYVRIRGDQLKARNGLYELRVTNE
                     LEEVLFVDKLQLVAVSHPEDTEVFPGEGLGSSLAARFQLFATRGARPPARATDEHGHD
                     MLARISQLDRHYADDFALEPIRGYAGEHALTLDLKEGMRDEGGRMNKHSSELSAPFMP
                     HPSSLIPSSGRTLLLLTGWTDYAFSSDNVAAAHRRLSLRPPALQVRDARGRWRTVIED
                     IGIPVGRPQTVVVDLTGKFLSQSREVRIVTNMRIYWDQILVDHSGTPSPTQLARLDPL
                     SADLRWRGFSAQTSPDGREPFGYDYTRVSLSSPWKVFTGRYTREGDVRELVQAVDDMF
                     VISKPGDELSIAFDASALAPLPRGWTRTFLLYADGYSKEMDINSASPEEVAPLPFHRM
                     REYPYRWPETFPDTPAHLDYLERYNTRIVGAPLPELVTRKKSDE"
     gene            3835..4863
                     /locus_tag="VGB76_03965"
     CDS             3835..4863
                     /locus_tag="VGB76_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY0098093.1"
                     /translation="MNHIRYSVAACQVDQPNPLERREMRRNTERMLSLIDSAVAGAAP
                     FLPVRLVVFPEFAHAAPVFPTLAELRQKLAVPIPNEHTELLAQKAREHNIFIQSGSML
                     EVDPKWPGAVFNTTCLIGREGILYKYRKVNTWIPYEVHTSPHDLEGYDEPLFPVAETE
                     IGKIGCAICYDWLFPEAIRQLAANGAEVLVRVSAYMDPWGATEPMDWWTLINRCRALE
                     NMAYVVAANQGASLRHYPPYSWPGGSQVVDYDGRLLAQASPGPGERIVVAPLDISALR
                     HERAARRGHHMLAHLRTEAYPVYSAHAYPPRRQGNAGEGEAPLSYERNNELIEAAKRA
                     LEEGAGQE"
     gene            complement(4909..5397)
                     /locus_tag="VGB76_03970"
     CDS             complement(4909..5397)
                     /locus_tag="VGB76_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098094.1"
                     /translation="MFCPSCGIALPQQLKYCNRCGAQLNATRETELVKLFEKRMESEM
                     EGLFWIIVFGLGLLLGGLVILKKGLGLGENLLLAYMILCSTVLLMYFGLGVWQVRRLA
                     RSSREASGTPSQQLGQVETNELNPATTLPTLEPVPSVIEQTTRTLEPAHRQSKTGGGS
                     AY"
     gene            5791..>6216
                     /locus_tag="VGB76_03975"
     CDS             5791..>6216
                     /locus_tag="VGB76_03975"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0098095.1"
                     /translation="MTFYRFTPRRASQLFVLLFCFAATAFPLSAQNNKGTILGTITDA
                     QGGLVNGAKVTAVNLSTNTPAEDTSNEEGLYTIPNLDPGMYRVTIEATGFKTVVNQSV
                     RLETNARLPIDAQLEVGSVGGDVVTVTDQGPLVESETSVR"
BASE COUNT         1204 a         2020 c         1862 g         1130 t
ORIGIN      
        1 atgtataaga ctgctgtgag aaaacgtcgt ctcttcattg tcgtgatcgt gttgacgggc
       61 ggcgttttca tgagcgcgct gctgtcgcat cgtgcaagca accgggtcgc cgtcgcgcag
      121 gagagtcgcc tgcccgccgc cgcgcgtgag gccgcctatc gcgccaacaa tctcggcgtg
      181 gctttgctcg aacagttcaa gcacagggaa ggcgcggaac agtttgaacg cgcgctcaaa
      241 ctcgatccgc aactcgcgct cgcgcgcatc aatctcgcca tcgcgctcta caacgtgccc
      301 gacgcggagg ccgccgcacg cgaagccgct agcgcgctca cgctcgcgcc ccgtgcgccg
      361 caggctctct acatcctcgc gctcgtcgca cgttcgcgta acaacacggc ggaagccgtc
      421 gaagccttcc gccgcgtgtt gcaggtggac gcacaggaca cgggcgcaaa cgtcaacctc
      481 ggtcaactct acgcgcaggg gcgcaactac gcggaggccg agcgcgcctt ccgcgccgcg
      541 ctcgcctcgg agccttacaa caccaccgcc ctctacggac tcgccaacgt cctcctgcgc
      601 tccaaccagc gcgaagaagg ccaacgccgc ttgcaggagt ttcaagtgtt gcgccagagc
      661 ggcgcgggca cgagcctcgg cacaaactat ctcgaacagg gacgctacgc cgaagccgtc
      721 acctccacgg gcgcggaagc cggactcgtt gacccgcgca cgcccgaagt cgtctacacg
      781 gatgcgaccg agggcgtcct gcccgtcgcg cccgcccccg ccacacgaac gccgccaagc
      841 gcaaacgcac gccgcacgcg ccccgccgcg ccggagcaat acgatgtgtc gctcagatcg
      901 cctgccacgc ggggcgtggc gaccctgttc gatttcgacg ttgacggcga cctcgacatc
      961 ttcgactcag catccggcgg gcagagactt tatcgcaacg acggcggtag gttcgtagac
     1021 gtgacggcca actccggcgc gctggcctca cgcgtggcgg gcgttggcct cgccgccgtc
     1081 gccggagatt atgataacga cgcgaagccc gatctcttcg tgctctacga agggcgcgtc
     1141 gcgctctatc acaacgaagg ggcggggaaa ttttccgacg ccaccctcgc cgcaggcatc
     1201 gcgtccgcca cgcaccattc catctccgcc gccttcgcag acgttgatca cgacggcgac
     1261 ctcgacatct tcaccggcgg gcacagcgac ctctcgaagg cgagcgtctc cacgcaggca
     1321 acggcaaaag caggcgagtt gaaattagat gccccgcccg cgccgaaccg cctgctgcgc
     1381 aacaacggcg acggcaaatt tacggacgtg acggcgggag cgaaagtcgc gggcgaacga
     1441 agccatacgg tcgccgtcgt cccgaccgat tacgacaatc gccgggatgt cgatctgctc
     1501 gtcgtcaact acgagggcgc gcccgcgctc tatcgcaacc tgcgcgacgg ttcgtttcga
     1561 gatgtggcgc gcgaagtggg cttggagttg aaagggcgat acctctcggc ggcggcgggc
     1621 gatgtcaaca aggacggctt cacagacttc tttttcggcg cggaggcatc ggcgggcacc
     1681 tttgctctga gcgacgggca gggacgtttc aacctcaagc ccgcgcccaa cgcgacccgc
     1741 gacgcgtctg ccgcgcagtt tctcgactac gacaacgacg gcctgctcga tctgttgatc
     1801 gacgcttccg gcggcatgcg cgtctggcgc aacctcggca acgactggtc tgatgtgagc
     1861 gagcgcgccg tggggcaaac gctgcgtgga gacggcgcgc gggagaaggc cggagaatcg
     1921 cgcgtgctgg cctcaggcga tctcgatgtt gacggcgaca cggatttgat agtccgcgac
     1981 acgaccggtc gcctgcgctt cgcgcgcaac gacggcggca atcgcaatcg cagtttgcgc
     2041 gtgcggctcg cgggtcgcgt gagcaaccgg agcggcgtcg aggcgaaggt cgagatgcgc
     2101 gcggggagtt tgtggcagaa gttggaagtt tacgcggcca gccctgcgcc cgcacccgcc
     2161 gacgtggttt tcggtctggg gcagcgcgag gcggcggacg ccgtgcgcgt gatctggcct
     2221 tcgggcacgg tgcaggcgga gacggagttc agcccggcgg caaccgtggc gggcgcgtcg
     2281 aatgagaagg gaagcgaccc gcgctctgcg acgaaggacg cacagcgcac gacggcagcc
     2341 gcaacttcaa acgcgacttt gaccgtcacc gaacttgatc gcaaaccttc ttcgtgtccc
     2401 tatctctaca cctggaacgg cgagcgtttc gagttcatca ctgacttcat gggcggcggt
     2461 gaactcggct actggctcgc gccgggcgtg cgctccgcgc ccgaccctga cgaatacgta
     2521 cgcatcagag gcgaccaact caaagcccgc aacggtcttt acgaattgcg cgtgacgaac
     2581 gaactcgaag aagtcctctt cgttgacaaa ctgcaactcg tcgccgtctc gcacccggaa
     2641 gacacggaag tctttcccgg cgaaggtctg ggcagctcgc tcgccgccag gttccaattg
     2701 ttcgccacgc gcggcgcacg cccgcccgca cgcgccacag acgaacacgg ccacgacatg
     2761 ctcgcgcgca tctcgcaact cgaccgccac tacgccgacg acttcgcgct cgaacctatt
     2821 cgcggctacg ccggagagca cgcgttaacg ctcgacctga aagaagggat gagggatgag
     2881 ggcggaagga tgaataagca ctcaagcgag ttaagcgctc ctttcatgcc tcatccctca
     2941 tccctcatcc cttcttccgg gcgcacgctt ctgctgctca cggggtggac ggattacgcc
     3001 ttctccagcg ataacgtcgc cgccgcgcat cgccgtttga gtctccgccc gcccgcgctg
     3061 caagtgagag acgcgcgcgg tcgctggcgc acggtcatcg aagacatagg gattcccgtc
     3121 gggcgaccgc aaaccgtcgt cgtcgatctc acagggaagt ttctttcaca gagccgtgag
     3181 gtgcgcatcg tcacgaacat gcgtatctac tgggaccaga ttttggttga ccattcgggc
     3241 acgccctctc ccacgcaact ggcgcggctc gaccccttga gcgcagacct gcgctggcgc
     3301 ggcttctctg cgcaaacgtc gccggacggg cgcgagcctt tcggctacga ttacacgcgc
     3361 gtctctctct cctcgccgtg gaaagttttc acaggccgct acacgcgcga gggcgacgtg
     3421 cgcgagttgg tgcaggcggt ggacgacatg ttcgtcatct cgaaaccggg cgatgaactg
     3481 tcaatcgcct tcgacgcgag cgcgcttgcg cctctgccgc gcggttggac gcgcaccttc
     3541 ctgctctacg ccgacggcta cagcaaagag atggacatca actcggcgag tcccgaagag
     3601 gttgcgccct tgcccttcca ccgcatgcgc gaatatccct atcgctggcc ggagacattc
     3661 cccgacacgc ccgcgcatct cgattatctc gaacgctaca acacgcgcat tgtcggcgca
     3721 cctttgcctg aattagtgac aagaaagaaa agtgatgagt gatgagtaat gagttagaac
     3781 caaagacttg tctttaactc gtcacttctt cctcgtccct gcttttaaaa ttttatgaac
     3841 catatacgtt attccgtcgc cgcttgtcag gttgatcaac cgaacccgct ggagcggcgg
     3901 gagatgcggc gcaacaccga gcggatgctg tcgctcattg attcggcggt ggcgggcgcg
     3961 gctccgtttt tgcccgtgcg cctcgtcgtc tttccggagt tcgcgcacgc ggctcccgtc
     4021 ttcccgacgc tcgcagagtt gcggcagaag ttggccgtgc ccatcccgaa cgagcacacg
     4081 gaactgttgg cgcaaaaggc gcgcgagcat aacatcttta tccagagcgg ctcgatgctc
     4141 gaagttgatc cgaagtggcc gggcgcggtc ttcaatacga cctgtctcat cgggcgggaa
     4201 ggcatcctgt ataaatatcg caaggtgaat acgtggattc cttacgaggt gcacaccagc
     4261 ccgcacgatc tggaaggcta cgacgaaccg ctctttcccg tcgccgagac ggagatcggc
     4321 aagatcggct gcgccatctg ttacgactgg ttgtttcccg aagcgatccg ccaactcgcc
     4381 gcgaacggcg cggaggtgct cgtgcgcgtc tcggcttaca tggacccgtg gggcgcgacc
     4441 gagccgatgg actggtggac gctcatcaat cgttgtcgcg cgttggaaaa tatggcttac
     4501 gtggttgccg ccaatcaggg cgcgagcctc aggcactacc cgccctattc gtggccgggc
     4561 ggaagtcagg tagtagatta cgacgggcga cttctggcgc aggcgtcgcc gggacccggc
     4621 gagcgcatcg tcgtcgcacc cctcgacatt tccgcgctca ggcacgaacg cgccgcccga
     4681 cgcgggcatc atatgctcgc gcacctgcgc acggaggctt atcccgtcta ttccgcgcac
     4741 gcctacccgc cgcgtcgtca aggcaacgct ggcgaagggg aggcaccgct ttcttacgag
     4801 cggaacaatg aattgatcga agcggcgaag cgcgcgctgg aagaaggggc gggacaggag
     4861 tagggaattt tgcggcggcg acgcagcggg agtgtgggcg gcgggcggtt aatatgcaga
     4921 cccgccgccc gttttgctct gccggtgtgc aggctccaac gtgcgcgtcg tctgctcgat
     4981 cacgctcggt acaggctcaa gcgtgggcag tgtcgtggcg ggattgagtt cgttcgtctc
     5041 aacttgtccg agttgctgcg acggcgtccc gctcgcctct ctggaactac gtgcgagacg
     5101 gcgtacctgc cagacgccga gtccaaagta catcagcagt accgtcgaac agagaatcat
     5161 gtaggcgagt aataagtttt cgccaagtcc tagacctttc ttcaagataa ccagtccgcc
     5221 gagcagcaac ccgaggccga agacgataat ccagaacagc ccctccattt cgctctccat
     5281 acgcttctcg aatagtttga ccaactcggt ttcgcgtgtg gcgttcaact gcgctccgca
     5341 gcgattgcaa tacttcaact gctgcggcag agctattccg caagacgggc aaaacatttc
     5401 ctgcgcctcc cctctctctc tggtgctgtg tgtaatacgt ctttttgcgc ggagacgttc
     5461 gacggcaaaa ccgcacgcgc ccgccgttta attgaaggcg cgctggccgc cgtcgcttgc
     5521 taacatccgg cgcagaggcg acggcgaaaa ggcgtccggc ggcggaaaaa ctgattgaca
     5581 gggagcaaat tctgtattaa aaatcttcgt tcgggcaagc aacaaactgt cctttcgcac
     5641 gttccgttca atcatctgaa ctaaatttct cgccggaaga gtagcttttc acaattcatc
     5701 gtcccccgcg ctcgacccct tcggagcaac ggtacgtcct tgagtgagca gcgattcgtc
     5761 aggcatctcc acagcacgag gccgaaagct atgacatttt acaggttcac cccgcgaaga
     5821 gcctctcaac tcttcgtcct actcttttgt ttcgccgcca cggcgttccc cctctctgct
     5881 caaaataata aaggcacgat tctcggtacg atcacggatg ctcagggcgg gctggtgaac
     5941 ggcgcgaaag tgaccgccgt taacctctcc accaacactc cagccgaaga cacctccaac
     6001 gaggaaggac tctacacgat ccccaacctc gaccccggca tgtatcgtgt gaccatcgag
     6061 gcgacgggct ttaaaaccgt cgtcaaccag tcggtcagac tcgaaaccaa cgcgcgcctg
     6121 cccattgacg cgcaactcga agtcggctcg gtcggcggcg atgtcgtcac cgtcacggat
     6181 caaggtccac tcgtcgagag cgagacgagc gtgcgc
//
LOCUS       DASWNS010000117         9500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_238591, whole genome shotgun sequence.
ACCESSION   DASWNS010000117 DASWNS010000000
VERSION     DASWNS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238591"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2670
                     /locus_tag="VGB76_03980"
     CDS             <1..2670
                     /locus_tag="VGB76_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY0098096.1"
                     /translation="NQLPLTPQRNSRRNEIIAIALFALGMLLTLCLVFFNPNDPSLNS
                     AGQEETRNLIGPVGAYVSDVMLQSVGLASYLLPLLLLLAAWRRFRTRRIHAPVSRIIG
                     LVTLLVSASAILALVSLPLLFDGRVSAGGFVGTFVSSNLASALGGIGSLVLLLALAAT
                     GLLLATNFSFAHAYERAVDAFGHPSAFIATLSTRFQTWRAERREHAQQRAEQRRAIRA
                     AHEEAQAEEKHLREKKTGAERVAEFMRESETPATAANNKTTQNASANVAIQRSAAAAE
                     ASNNSTTNASPQPTIQNDLDEAFEEFADGDDDNALESPGNASAGSNASAATRKSNAKL
                     DAQLKEEDAELSPSAFIGEEDEEVAEMVASVSVVRTENTEEESVKQKEDKATREARRA
                     EKYAEAMSGYDMPGVDFLNAPPPRHEQADEELRNLATRVAEKCKEFNVTGKIEYICPG
                     PVVTTYEFKPDPGVKYSRVTGLVDDLCLALKAESIRIDRVPGKAHVGIEVPNHQRDNI
                     MLREVIESRHFKDSTSKLTLALGKTIDGLNYVADLARMPHLLIAGATGTGKSVCLNSL
                     VVSILYKARPDEVKFIMIDPKRLELGLYADIPHLATPIITDPKRAANALKWAVAQMEA
                     RYKELAKWGVRNIDGFNVEVLRRNMAEDYDDEGQPWKTLPYIVIIVDELADLMMTAGH
                     DVEEAITRLAQMARAVGIHLVLATQRPSVDVITGLIKANFPSRIALRTSSKVDSRTII
                     DANGAEQLLGRGDMLFLPPGTSRLIRVHGAYLDESEVNRIVSHIKGQGEPLYDETITQ
                     SEEESGGGGGVGGERDELFEQALRICVEMKRASTSVLQRRLRIGYGRAAAMLDAMERE
                     GYIGQADGARPRPITGRAYETVSHWDDQLREEG"
     gene            2935..3207
                     /locus_tag="VGB76_03985"
     CDS             2935..3207
                     /locus_tag="VGB76_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY0098097.1"
                     /translation="MPRRAGVVRNLTTSELAPTPSSAQATTRIRINTASRAELERLPG
                     IGEGLAARIIEHRERHGAFRRVEHLIIVRGISERRFEALRPFVTAE"
     gene            3242..4630
                     /locus_tag="VGB76_03990"
     CDS             3242..4630
                     /locus_tag="VGB76_03990"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEY0098098.1"
                     /translation="MIAGRDELRRAARSIFDATLAHADARLAVRRALNFTGGRLNIFD
                     TEFDLRDASRAVYSIAVGKAGEAMASALDEVLGDALAGGVVSTPPLNHPSRARLSKRW
                     RTFAGGHPLPNEASLDAARAAFALLHEADAQANALVIFLISGGGSAMLEWPRDESITL
                     AELQAANRALVSCGAEINEINSVRRAFSSVKGGGLSARAGRAAQVSLIISDTNRDEPF
                     NVASGLTYAFPPSSHAPPDPAEIIERYKLASSLPASILRAVKQTHARPEASTYAPLPL
                     RLHHVLLDNESALEHAAATASALGFASEIAGDVCEGSVEAGTATLVSRLFDLREREGM
                     NGRPLCLISGGEFSCPVRGAGVGGRNAETVLRCAFEIEARAAHTSSGAPIHVVALSAG
                     TDGIDGNSPAAGALCDETTLSRARALSLDARKFLEASDAHTFFAALGDAIHTGATGTN
                     VRDLRLLLCESK"
     gene            complement(4773..5141)
                     /locus_tag="VGB76_03995"
     CDS             complement(4773..5141)
                     /locus_tag="VGB76_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEY0098099.1"
                     /translation="MPESETTNTSPLYDERPWGSFTVLDEGENFKVKRIEVLPGKRLS
                     YQKHAQRAEHWMVVAGAGLVTLDDEESTVRTGETIDVPIGAKHRIENPGTEKLIFIEI
                     QRGAYLGEDDIVRLSDDFGR"
     gene            complement(5308..8076)
                     /locus_tag="VGB76_04000"
     CDS             complement(5308..8076)
                     /locus_tag="VGB76_04000"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY0098100.1"
                     /translation="MSTVSPHRPAFNQHPLAWLAASFANGILLAYLASPPLYLLTVSG
                     ALCTLLALFACKRRQGTLATLSLVLAFVCMGACVSQVEARRSVGVERVRRLYEEGRVV
                     SGEPLEVTGVIVRAPEFAPDGFYLTLRSERVRVRGTEQAAAGAVELFAPAGDAATRVK
                     YEELELRRGARVRVLVALERAEKFRNPGGNSWPEFLERRGIDAAGTIKSPLLVERLDD
                     ERVLVPLVWLDAWRERLHLRMSELFRAETAGVLQAALLGNRYGLSRASAERFREGGTF
                     HVLVISGLHISFIGVVVLLLMRRVLPRRRAWQVCVACVVLWAYAVAVGAESSVVRAAL
                     MFTLVALAPVAGRRGATLNALGGAALVLLIHRPAELFDPSFQLTFLSVLGIVALGWPL
                     IERLRAVGAWHPTRATPHPPVCPRAFLVLGESLFWSERAWRRELERNVYSYRLFKAGA
                     GEWFERWRVQRVWRYAFGAMIVSASVQLMLLPFFVLYFHRVSVASLFLNIFVGVLMAA
                     ASFGALLAMLLTTLSADAAAPLVWFVEWTNWLMTHSVDPFAQAGVASLRLPEYTGRAA
                     LVYALYYVPLAVLLRSLARWQPLQTPAASREEQGQRARRTTARRFAWAGCAICLCLII
                     LHPLSAGRTSGSGGRLRLDLLDVGQGDAALLTCPDGATLLVDAGGRARFDATRAGDVE
                     DATATRFERDGRDIGEAVVAEYLWWRGLERVDYILATHAHADHMSGLNDIARNFGVRA
                     ALIGRISATDEAEFARFAQTLRRERVPVELIARGDRLRFGSATVDVLWPPHMAGDAPQ
                     AFSENDSSLVLRVRYGERVFLLTGDIEREAEAALVAAGDALQSDLVKVAHHGSRTSSS
                     ESFIAATRPSVAVISVGHTSPYGHPDANVVARWRAAGAQVLQTGRRGTVTVSTDGHDL
                     RVETYAHE"
     gene            complement(8252..>9500)
                     /locus_tag="VGB76_04005"
     CDS             complement(8252..>9500)
                     /locus_tag="VGB76_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098101.1"
                     /translation="FNVRRLRTPFFFHLCLLIASVAAVNASARAQDAAEENAADPAVR
                     TQVAINEVKSGDPLMANPLTITVNGMTKALEDRKITAGDLILTLNGHPLNGLKGSQLN
                     ENTLEFRPKRTDETKDTWNELLGSPKFREAARQMTVGLQTPKGEEFATPLVASFHIFS
                     PFWANISLIGLLLMFGIFFWLAKTTNIIRDSNPPDPPPGKRRPYSLARTQAAFWFFLV
                     IASFLLIYLITNDYNTITEQALILIGIGTGTALGAAMIDASKRDTSNDELAALVPERD
                     KLQTEVAELENQQTELANRIRAAGEAATDEDKQALSALKIGLSEKRAMLAAAEKKVAD
                     AKSGLSTPVSEGLKKDLLSDPDGEISFHRFQIVVLTIILGTMFCVGVYRALAMPQFDG
                     TLLALMGISSGTYLGFKIPERQS"
BASE COUNT         1838 a         3186 c         2778 g         1698 t
ORIGIN      
        1 aaccaactgc cgctgacacc gcagcgcaac tctcgccgca acgagatcat cgccatcgct
       61 ctcttcgcgc tcggcatgct cttgacgctc tgcctcgtct tcttcaaccc gaacgatccc
      121 tctctaaact ccgccgggca ggaagagacg cgcaacctga tcggtcccgt cggcgcttat
      181 gtctcggacg tgatgctgca atcggtcggc ctcgcctcct acctgctgcc gctcctgctc
      241 ctgctcgcag cgtggcgtcg ctttcgcacg cgacgcatcc acgcgcccgt ctcgcgcatc
      301 atcggcctcg tcacgctctt agtctcggct tcggccatcc tcgcactcgt cagtctgccg
      361 ctgctcttcg acgggcgcgt gagcgcgggc ggattcgtcg gcacgttcgt ctcttcgaat
      421 ctggcgagcg cgctcggcgg catcggttcg ctcgtcctgc tgctcgcgct cgccgccaca
      481 gggcttctgc tcgccaccaa cttctctttc gcacatgcct acgaacgcgc ggttgacgcc
      541 ttcggccacc cgtcagcttt catcgccacc ctctcgacgc gcttccagac gtggcgcgcg
      601 gagcgacgcg agcatgcgca gcaacgcgcc gaacagcgtc gtgccatccg cgccgcgcac
      661 gaagaggctc aggccgaaga gaagcatttg cgcgagaaga agaccggcgc ggaacgcgtc
      721 gcggagttca tgcgcgaatc ggagactcct gcgaccgccg ccaacaacaa aacgacgcag
      781 aacgcttccg ccaacgtcgc catccaacgc tccgccgctg ccgccgaagc gagcaacaac
      841 agcacgacga acgcgtcgcc ccagcccacg attcaaaacg atctggacga agccttcgaa
      901 gagtttgcag acggagacga cgacaacgcg ttggaatcgc cgggcaatgc ttccgccggg
      961 tcgaacgcta gcgcagcgac gcgcaaatca aacgcgaagc tcgacgcaca gttgaaagaa
     1021 gaagacgcgg agctttcgcc gagcgcattt atcggcgagg aagacgaaga agtggcggag
     1081 atggtcgctt ccgtgtcggt cgtgcgcacg gagaacactg aggaagagtc cgtcaagcag
     1141 aaagaggaca aggccacgcg cgaggcgagg cgcgcagaga agtacgcgga ggcaatgagc
     1201 ggctacgaca tgccgggtgt tgatttcctg aacgccccgc ccccgcgcca cgagcaggcc
     1261 gacgaggaac tgcgcaatct cgccacgcgc gtcgcggaga agtgcaagga gtttaacgtc
     1321 acgggcaaga tcgaatacat ctgccccggc cctgtcgtca cgacttacga attcaaaccc
     1381 gaccccggcg tcaagtattc gcgcgtcaca ggactcgtgg acgatctctg cctcgcgctc
     1441 aaggccgagt ccatacgcat cgaccgcgtg cccggcaagg cgcacgtcgg catcgaagtg
     1501 cccaatcacc agcgcgacaa catcatgctc cgcgaagtga tcgaatcgcg ccacttcaaa
     1561 gactcgactt caaaactcac gctcgcgctc ggcaagacca tcgacggctt aaactacgtc
     1621 gcagacctcg cgcgcatgcc gcaccttttg atcgcgggcg caaccggcac aggcaagtct
     1681 gtctgtctga actcgctcgt cgtttccatt ctttataaag cgcgtcctga tgaagtcaaa
     1741 ttcatcatga tcgatccgaa gcgtctggag cttggcctct atgcggacat cccgcatctc
     1801 gccacgccga tcatcaccga tccgaagcgc gcggcaaacg ctctgaagtg ggcggtggcg
     1861 cagatggagg cgcgctacaa ggaactcgcc aagtggggcg tgcgcaacat tgacggcttc
     1921 aacgtggaag tgttgcgccg taacatggcc gaggactacg acgacgaagg acagccctgg
     1981 aagacgctgc cttacatcgt cattatcgtg gatgaacttg cagacttgat gatgaccgcc
     2041 gggcatgacg tagaagaagc gatcacgcgt ctggcgcaga tggcgcgcgc cgtcggcatc
     2101 catctcgtgc tcgccacgca gaggccgtcc gtggatgtca tcaccggact catcaaggct
     2161 aacttccctt cgcgcatcgc cctgcgcacg tcttccaaag ttgactcgcg cacgatcatc
     2221 gacgccaacg gcgcggagca acttctgggg cgcggcgaca tgctcttcct tccgcccgga
     2281 acctctcgcc tgatccgcgt ccacggcgca tacctggacg agtcggaggt caaccgtatc
     2341 gtctcgcaca tcaaggggca gggcgaaccg ctctacgacg aaaccatcac gcagtcggaa
     2401 gaagagtcag gcggcggggg cggcgtgggc ggcgaacgcg acgagctttt cgagcaagcc
     2461 ctgcgcatct gcgtcgaaat gaaacgcgcc tccacctccg tgctccaacg ccgcctgcgc
     2521 atcggctacg gacgcgccgc cgccatgctc gacgccatgg aacgcgaagg ctacatcggt
     2581 caggccgacg gcgcacgccc ccgccccatc acagggcgcg cctacgaaac cgtctctcat
     2641 tgggacgatc aactgcgcga agagggctga aacaactttt ccacagaatc tgtggaaaag
     2701 cctgtggaaa aatcgagttt ttgcgttaca agttcctgac aaatttagac ttctagcatt
     2761 ttgcacatct tcgcggcaaa gtttgccatt cctgtttcaa aggccgttaa agctgtgtct
     2821 agtcaacgct ttggagattg cacgcgcctt ttgcgggcaa ggcgcgtgcg ttctgtcccg
     2881 cccgtgtttt tatgctacgc gcttgcgctc gcgtgcgtct cgtcgtgtgt taagttgccc
     2941 cgtcgcgccg gagttgtacg taatctgaca acatccgaac tcgcgcccac gccctcctct
     3001 gcgcaggcca cgactcgcat ccgtatcaac acggcttcgc gcgcagagtt ggagcggctg
     3061 ccgggtatcg gcgaaggact cgcggctcgc atcatcgaac atcgcgaacg gcacggcgcg
     3121 tttcgccgcg tcgaacatct catcatcgtg cgcggcatca gcgagcgacg cttcgaggct
     3181 ctgcgcccgt tcgtcaccgc cgagtgattc gcctttacca tccgacctcg ctcctttaac
     3241 tgtgattgcc ggaagagacg aactgcgccg cgccgcgcgc tccatcttcg acgccacgct
     3301 ggcgcacgcc gacgcgcgcc tcgccgtccg ccgcgcgctc aacttcacag gcgggcgact
     3361 caacatcttc gacaccgaat ttgatctacg cgacgcgtcg cgtgcggtct actcaatcgc
     3421 cgtcggcaaa gccggagagg cgatggcctc cgcgctggat gaagtgttgg gcgacgcgct
     3481 cgcgggcggt gtcgtctcca cgccgcctct gaaccatccc tcacgcgccc gtttatccaa
     3541 acgctggcgc actttcgcgg gcggtcaccc tttgccgaac gaagcgagcc tcgacgcggc
     3601 gcgcgctgcc ttcgccctgt tgcacgaggc cgacgcacaa gcgaacgcgc tcgtcatctt
     3661 cctcatctcc ggcggcggtt cggcgatgct cgaatggccg cgcgatgagt ccatcacgct
     3721 cgcggagttg caggcggcga accgcgcgct tgtctcctgc ggcgcggaga ttaatgagat
     3781 caactccgtg cgtcgtgcgt tctcttccgt gaaaggcggc ggactatctg cgcgtgcggg
     3841 gcgcgcggcg caagtctcgc tcatcatctc cgacacgaac cgggacgagc ctttcaacgt
     3901 ggcctccggc ctgacctacg ctttcccgcc ttcctcgcat gcgccacccg acccggcaga
     3961 gatcatcgag cgttacaaac tcgcctcatc actccccgcc tcgatcctgc gcgccgtcaa
     4021 acaaacgcac gcacgcccgg aggcttcaac ttacgcgccg ctgccgttgc gcctgcatca
     4081 tgtgctgctc gataacgaaa gcgcgcttga gcatgcggca gcgacggcct ccgcgctcgg
     4141 cttcgcgtct gagattgccg gagacgtgtg cgaaggttca gtcgaagcag gaacggccac
     4201 gctcgtctcg cgtcttttcg atttgcgaga gcgtgagggg atgaacgggc ggcctctgtg
     4261 tctcatctcc ggcggcgagt tttcgtgtcc ggtgcgaggc gcaggcgtag gcgggcgcaa
     4321 tgccgagaca gtcctgcgtt gcgcgtttga gatagaggct cgcgctgcgc acacatcgtc
     4381 cggcgcgcca attcatgtcg tcgctctcag cgcgggtact gacggtattg acggcaacag
     4441 tccggcggcg ggagccttgt gcgatgagac gacgctttca cgcgcccgcg cgctttcgct
     4501 cgatgcacgg aagtttctcg aagcgagcga cgcacacact tttttcgccg cgctcggcga
     4561 cgccatccac accggcgcaa ccggcacgaa cgtacgcgac ctgcgactgc tcttgtgtga
     4621 aagtaaataa gggtgagggg cgagcgtact caagtcgaaa gcggcgggac gaaagttaag
     4681 acgaagcctc cggcatgatg caactcggaa atccgcatca cgccggaggc tgaaacacgc
     4741 gctcgccccg ccgctttcga ctctctattc catcatcgtc cgaagtcgtc gctgagacgc
     4801 acgatgtcgt cttcgccgag atacgcgccg cgttgaattt cgatgaagat caatttctcc
     4861 gtaccgggat tttcgatgcg gtgctttgcg ccgatgggca cgtcaatcgt ctcgcccgtc
     4921 cgcaccgtgc tctcttcgtc gtccaaagtc accagccctg cgcccgccac caccatccag
     4981 tgctccgcgc gctgcgcgtg cttctggtaa ctcagacgct tccccggcaa gacttcaata
     5041 cgcttgacct taaaattttc gccctcgtcg agcacggtaa acgaacccca cgggcgttcg
     5101 tcataaagcg gcgaagtgtt cgtcgtctcg gattccggca tgcttcctcc ctgccccgtc
     5161 ctacctcttt aattaactga cgcgcgcatc agccgacagc aatatatccg ttcgaacgga
     5221 ttgattcaac gtagcgttgc agcgtcgccg ttgaccattc cgttgaccat tccgttttca
     5281 agtcgctgcc ttcaagcttc acaggcatca ttcgtgcgcg taagtctcca cgcgcaagtc
     5341 gtgcccgtcg gtgctgacgg ttaccgtgcc gcgccgcccg gtctgtagaa cctgcgcgcc
     5401 cgctgcacgc cagcgcgcaa cgacgttcgc atccgggtgt ccgtagggcg acgtgtggcc
     5461 gaccgagatg acggcgacgg aggggcgtgt cgccgcgatg aatgattccg acgaagaggt
     5521 gcggcttccg tgatgcgcta ctttcacgag gtcgctttgc agcgcgtcgc ctgccgccac
     5581 gagggcggcc tccgcctcac gctcgatgtc gcccgtcaag agaaacacgc gctcgccgta
     5641 gcgcacgcgg agcaccagag aactgtcgtt ctccgaaaac gcttgcggcg cgtcacccgc
     5701 catgtgcggc ggccagagca catccaccgt cgcggagccg aacctgagcc ggtcgccgcg
     5761 cgcgatgagt tcgaccggca cgcgctcgcg tcggagcgtc tgcgcgaagc gcgcaaactc
     5821 cgcttcgtcc gttgcactaa tacgtccgat gagcgcggcg cgcactccga aattacgcgc
     5881 gatgtcgttt aagccgctca tgtgatcggc gtgcgcgtgc gtcgcgagaa tgtagtccac
     5941 acgctcaagc ccgcgccacc acaggtattc cgcgacgacc gcctcgccga tgtcgcgccc
     6001 gtcgcgttca aacctcgtgg ccgtcgcatc ttcaacgtcg cccgcacgcg tcgcgtcgaa
     6061 ccgggcgcgc ccgcccgcgt caaccaacag agtcgcgccg tccggacacg ttaacagcgc
     6121 ggcatcgccc tgccccacgt cgagcaggtc gagccgcaga cgcccgccgc tgccgctggt
     6181 acgccccgcg ctgagcggat gcaggatgat caggcagaga cagatcgcgc agcccgccca
     6241 ggcgaaccgt cgcgctgtcg tgcgtcgcgc gcgttggcct tgttcttccc gcgaggctgc
     6301 gggcgtttgt aaaggttgcc agcgcgcgag gctcctgagc aagaccgcga gcggcacgta
     6361 gtaaagcgcg tagacgagcg cggcgcggcc tgtgtattcc ggcaacctga gcgaagcgac
     6421 gcccgcctgc gcgaacgggt caacgctatg cgtcatcagc cagttcgtcc attcgacgaa
     6481 ccacacgagc ggcgcggcag catccgcgct caacgttgtg agcaacatcg ccagcagcgc
     6541 gccgaaactt gccgccgcca tcaacacgcc gacgaagata ttcaaaaaca gcgacgccac
     6601 cgacacgcga tgaaaataga ggacgaagaa aggcagcaac atcaactgca cgctcgccga
     6661 gacgatcatc gcgccgaacg cgtaacgcca cacgcgctgc acgcgccaac gctcaaacca
     6721 ttcgcccgcg cccgctttga agagacgata gctatagacg tttcgctcca actcgcgccg
     6781 ccacgcgcgc tcgctccaaa acaggctctc gccgagcaca agaaacgcgc gcgggcagac
     6841 gggcgggtgc ggcgtcgcac gcgtcggatg ccatgccccg accgcgcgca agcgttcgat
     6901 gagcggccag ccgagcgcga cgatgccgag caccgaaaga aaagtgagtt ggaacgacgg
     6961 gtcgaaaagc tctgcgggac gatggatgag gagcaccagc gctgcgccgc cgagcgcgtt
     7021 gagcgtcgcc ccgcgccgcc cggccacagg cgcgagcgca accaaggtga acatcaacgc
     7081 ggcgcgcacg acggacgact ccgcgccgac cgccacagcg taagcccata acacgacaca
     7141 cgccacgcag acctgccacg cccgccttcg cggcagcacc cgccgcatca agagcagcac
     7201 gacgaccccg atgaaactga tgtgcaggcc gctgatgacg agcacatgaa aagtgccgcc
     7261 ctcacgaaaa cgctccgcgc tcgcacgcga cagcccgtaa cgattgccga gcagcgcggc
     7321 ctgcaacacg cctgccgtct ccgccctgaa cagttccgac atacgcaggt gcagacgctc
     7381 gcgccatgcg tccagccaca cgagcggcac gagcacgcgt tcgtcgtcca gacgctccac
     7441 gagcaaagga ctcttgatcg tgcccgccgc atctattccg cgtcgctcca aaaactccgg
     7501 ccacgagttg ccgcccggat tacgaaactt ctccgcgcgt tcgagcgcca ccagcacgcg
     7561 cacgcgcgcc ccgcgccgca gttccaactc ttcgtattta acccgcgtcg ccgcatcgcc
     7621 ggcgggcgca aacagttcga ctgcgcccgc tgccgcctgc tccgtgccgc gcacgcgcac
     7681 gcgttcgctt cgcagcgtga gataaaaacc gtccggcgca aactccggcg cgcgcacaat
     7741 aacgcccgtc acctccaacg gctcgcccga aacgacgcgc ccctcttcat aaagacgccg
     7801 cacgcgttcg acgccgacgc tgcggcgcgc ttcaacctgc gagacgcacg cgcccatgca
     7861 cacgaacgcg agcactaagc tcaacgtggc gagcgtgcct tgtctccgtt tgcaggcgaa
     7921 gagggcgagc agcgtgcaga gtgcgccgga aaccgtaagg aggtagagcg gcggcgaggc
     7981 gagataagcg agcagtatgc cgttggcgaa agatgccgcc agccatgcga gcgggtgctg
     8041 attgaaggcc ggtctatgag gtgaaaccgt actcatgcca ctcggcgtcg gacatttata
     8101 acgaactaca actgcgtccg gctctccgtt gcgtccggct cgctgcgccc gccctgcgtc
     8161 acctgcgtct ctgtagtgaa tgtcttacaa aagcactcta tcaccgcagg cacagaggat
     8221 acagggcggg cacagtggaa agagcgttcg ctcaactttg ccgttcggga atcttgaagc
     8281 cgagataggt gcccgaactg atgcccatca gggcgagcag cgtgccgtcg aattgcggca
     8341 tcgccagcgc acgatagacg ccgacgcaga acatcgtccc cagaatgatc gtcaacacga
     8401 cgatctgaaa acggtgaaaa ctgatctcgc cgtcagggtc ggacaacagg tctttcttga
     8461 gtccttccga caccggcgtg ctcaaacctg atttcgcgtc cgcaaccttc ttctccgccg
     8521 ccgcgagcat cgcgcgtttc tcgctcagac ctattttcag cgcgctcaga gcttgtttgt
     8581 cttcgtccgt ggccgcctcg cccgccgccc ttatccggtt cgcaagttcc gtctgctggt
     8641 tctccagttc cgccacctcg gtctggagtt tatccctttc gggcacgagc gcggccagtt
     8701 cgtcgttcga ggtgtcgcgc ttcgaggcat cgatcatcgc ggctccgagg gctgtgcccg
     8761 tgccgatgcc gatcaagatg agcgcctgct ccgtgatcgt gttgtaatcg ttcgtgatca
     8821 aatagatcag caggaaggag gcgataacca gaaagaacca gaaagccgcc tgcgtccgtg
     8881 ccagactgta aggtcttctc tttcccggcg gcggatcggg cgggttggaa tcgcgtatga
     8941 tgttggtcgt cttcgccagc cagaagaaga tgccgaacat caagagcaac ccgatgaggc
     9001 tgatgtttgc ccagaaggga ctgaagatgt gaaaactggc gacgagcggt gtagcgaact
     9061 cctcaccttt cggcgtctgc aacccgaccg tcatctgccg cgcggcctct ctgaacttgg
     9121 ggctgccgag cagttcgttc caggtatctt tcgtctcatc ggttcgcttg ggtctgaact
     9181 ccagtgtgtt ttcgttgagc tggctccctt tgagtccgtt gagcgggtgg ccgttcagag
     9241 tcaaaatcag atcgcccgca gtgatcttcc tgtcctccaa tgctttcgtc attccgttga
     9301 cagttatggt cagcggatta gccatcaggg ggtcgcccga tttcacttcg ttaatggcta
     9361 cttgcgtgcg cacggcgggg tcggcggcat tctcttccgc agcatcctgc gcccgtgcgg
     9421 aagcgttaac ggcggcgacc gacgcgatga gcaaacacag gtgaaaaaag aagggggttc
     9481 tgaggcgtcg aacgttgaag
//
LOCUS       DASWNS010000118        15620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_108992, whole genome shotgun sequence.
ACCESSION   DASWNS010000118 DASWNS010000000
VERSION     DASWNS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15620
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108992"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1514)
                     /locus_tag="VGB76_04010"
     CDS             complement(183..1514)
                     /locus_tag="VGB76_04010"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY0098102.1"
                     /translation="MIKKRIAAACASLLLLLLVGCSKPARRVEQASSAASSTTPNSAK
                     DVRRITSVEPVLVSVKDTDAAEPALAAAGDGSVFVAWVEHRAGGEADVWLAHLDGEAK
                     PLGAPVRVNPQAGAATAWHGDPPTIAVAPDSTVYVGWTARVPGAKHASTLYLSASRDG
                     GRSFASPVKVNDDERPAAHGMHSLAAAPDGRIYMAWLDERNVAPPPPPKPNSTHRHKE
                     SNREIFFAASTDGGRTFEANRRIASEVCPCCKTSLAAGADGRVYASWRQVLPGEFRHI
                     AVAASTDGGRTFSSPAIVNDDKWQIAGCPVSGAALSLTNDGTLSVLWYTAGEAGTPGL
                     YQTQSLDGGRTFAPRRAFARSSGRGTPVLLAGQGGDHLMAVWEERGEGAATRIAATHF
                     KDGVETPPADTLASAGELPVATKTGQQLFVAYVSETDARRTVWLVRARLEE"
     gene            1779..4277
                     /locus_tag="VGB76_04015"
     CDS             1779..4277
                     /locus_tag="VGB76_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEY0098103.1"
                     /translation="MKQQVKTEEQQQQGAPEQKAEAHPSHYEPAKQDLKSERELRAEV
                     AAASEAGSQPDAAQELKEPAHASRRAQPPEDFTGIKISHAEKAAGGLPAIMSAMRHSW
                     SEMGATRTLKTLTRINQKGGFDCPGCAWPEPDGERTHFEFCENGAKHVADEATTKRIT
                     PDFFRKWSLTELSRQSDMWLNDQGRLTHPMLLRRGTDFYEPVSWDAAFALIAGELHAL
                     ASPDEAVFYTSGRTSNEAAFLYQLFVRKFGTNNLPDCSNMCHESSGSALTESIGVGKG
                     TVTLEDFAEAEAIFVIGQNPGTNHPRMLSTLQAAKRRGCRIVHINPLPEAGMSRFKHP
                     QEVFDTLIGSGTQLADLFLQVRINGDVALLQGIAKEVLEAESQRAGEVLDHDFIAAHT
                     TGFEAFAASLGKVVWEEITESCGIGREEIREAARIFIESKRTIFCWAMGLTQHKNAVA
                     NIQEIVNLLLLRGQIGQTGAGVCPVRGHSNVQGDRTMGIWERPTAAFLDALGREFDFA
                     PPRAHGFDTVEAIKAMHAGKAKLFFAMGGNFLSATPDTEYTAEALRRLRLTVQVSTKL
                     NRAHLITGEQALILPCLGRTEIDRQATGEQFVSAENSMGVVQSSRGNLEPASAELLSE
                     VAIVARLADATLGGTRRDRINWLELAADYDLIRERISRVVVGFENYNERVRRPGGFYL
                     PNLARAREWKTASGKAQFTVHELPHHALAPGQFLMMTIRSHDQFNTTIYGLDDRYRGI
                     RNERRVVLLNPEDISEAGLDAGSNVDLVSHFEGEERIARRFIVVPYSIPRRSAATYFP
                     ETNVLVPLGSVADKSNTPASKSVVISLRPCVAAT"
     gene            4407..4928
                     /locus_tag="VGB76_04020"
     CDS             4407..4928
                     /locus_tag="VGB76_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098104.1"
                     /translation="MATSRLGTYSLFLAVILWGTVLGGIVYSHLVYFPVYLSALPASS
                     VVVNGTYGLHEAIFWITVHPLLILSLILALVLNWKNRSRRKLILISCAIYFLVLVITQ
                     IYFIPELIAFEQSPASNVSASEWFARGQRWQRLSWLRGVVMYAAILPLLLALTRAAPD
                     AGARAFSATDARA"
     gene            complement(4903..5694)
                     /locus_tag="VGB76_04025"
     CDS             complement(4903..5694)
                     /locus_tag="VGB76_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0098105.1"
                     /translation="MANQMGFSLLQGVVLFASAFIAGMINSIAGGGTLLTFPTLIWLG
                     LDPKEANATSTVALWPGLVGGLFGFRRELEKSRPLLIRLGITSFFGGAVGAALLIRTP
                     SETFARLVPFLIFFATLLFMAQEPITRWLRLEATPAAGRQRGWWTGAILFQFCAAVYG
                     GYFGAGNGILMLAALGLLGISDIHRANGLKNFLGLTLNSVAVVAFALSGLVRWTPALL
                     MAAGAIMGGYYGAEIARKLGRTFVRRAVVLIGLTIGCLMLWRLSR"
     gene            5808..7205
                     /locus_tag="VGB76_04030"
     CDS             5808..7205
                     /locus_tag="VGB76_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY0098106.1"
                     /translation="MAIASVNPATGEKLREFAPLTEAEIEEKLERAARAFQTHRRTSF
                     AERAERMLRAADILETEKERFARLMTTEMGKPVRGAREEVAKCALVCRYYAEHAGSFL
                     ADETVETSATRSFVHYEPLGAVLAVMPWNFPFWQVFRFAAPALMAGNTGLLKHASNVP
                     QCALAIEEIFLRAGFPVGVFQTLLLNSDQVARVIDDARVRAATLTGSEQAGRQVASRA
                     GGEIKKTVLELGGSDPFIVMPSADFESAVKTAVKARTINNGQSCIAAKRFIVHTEIYE
                     EFARRFVAELEALKVGDPSDETTEIGPLATESIVAELDEQVRKSIETGARTLTGGERL
                     QRAGNYYAPTVLTDIPKDSPAYGEEFFGPVALLFRVGSIDEAIALANDTTFGLGASAW
                     TRAEDERARFVDELEAGAVFINAMVASDPRLPFGGVKRSGYGRELGAFGIREFVNIKT
                     VWLSDDGGERRTETE"
     gene            7513..8184
                     /locus_tag="VGB76_04035"
     CDS             7513..8184
                     /locus_tag="VGB76_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0098107.1"
                     /translation="MIRLRNVEKVYEQGVNRTYVLRRVDMDVREGEFVSIMGPSGAGK
                     STLLSILGMFDSAWTGEYYFWDAAVHKLNKKERAELNKRYIGFVFQSYHLLDHMTVYE
                     NLDMPLSYKDVKKSERQSIVGDVLDRFQIVGKKDLYPNQLSGGQQQLVAVARALISNP
                     KLLLADEPTGNLHSNQGREIMELFKRLNEEGTTIIQVTHSEQNAAYGHRIVQLADGWV
                     VKEEK"
     gene            8431..9687
                     /locus_tag="VGB76_04040"
     CDS             8431..9687
                     /locus_tag="VGB76_04040"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0098108.1"
                     /translation="MDVPRGKSSARKRRVRVALYAIIAIVAIAAITFGVSRMKPAAPS
                     VERSTALIDTVKRGQMLRQVRGIGTLVPQDIRVIAAATEGRVERVFVQPGTEVTAGTV
                     LIELSNPELQQGTVDIEYQVRAAEADYNNIRARLDSERMSQQAAAATVQAEYQQAKLQ
                     SDTDEELAKDGLIPAINLKLSRVRVADLANRYSLEKKRLDGTAKSAQAQLASQQARIA
                     QLKAQLQLRQTQVATLRVLAGTNGVLQQMQVEVGQQVQPGTNLARVVEPQNLKAELRI
                     AETQAKDITLGQQASIDTRNGIIPGHVVRIDPASENSTVRIDVALDAPLPQGARPDLS
                     VDGTIELERLDNILYVSRPAFGQAQSTIGMFKLETGGQFATRVQVKLGRSSVNTIEIL
                     EGLQEGDQVILSDTSQWDAFNRIRLN"
     gene            9793..10554
                     /locus_tag="VGB76_04045"
     CDS             9793..10554
                     /locus_tag="VGB76_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0098109.1"
                     /translation="MSASKQPLIRLQDVKKVFFTDEVETHALAGTHLDIANGEYVSIT
                     GPSGCGKSTLLSILGLLDSPTEGKYWLNGKLVSDLSLGERAHIRNREVGFIFQSFNLI
                     GDLSVYENVELPLTYRGMSNADRKARVTDALEKVGMAHRAKHLPSQLSGGQQQRVAVA
                     RAVAGQPLILLADEPTGNLDSKNGEAVMDLLRELHRGGATICMVTHDPRYATFADRAI
                     HLFDGRVVKEEMVSFKDADDEAAQAGQAGTQVGVA"
     gene            10554..13001
                     /locus_tag="VGB76_04050"
     CDS             10554..13001
                     /locus_tag="VGB76_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098110.1"
                     /translation="MGNLWQDVRYGFRTLVKSPGFTLIALMALALGIGANTAIFSVVN
                     AVLLRDLPYRESDQVMIVWEQNRARGGRNNVASLANFLDWQQQANSFQEMAAFYDAAF
                     NLTGEGNPEEIPAQVTSGNLLTMLGTQPALGQVYTKDEAEPGRDDVVVISHGLWQRRF
                     GGAGDIIGKKISLNGQNVTVIGVMPQGFKWFMKEGSRTGKAAEIWTPTNFASVLNGAT
                     KGRGRFITVAGRLKPGVTREQAQAEMSTIASRLEQQHAQFNTNMGIHVVPVREQLAGE
                     IKIALYVLLGAVGFVLLIACANVANLMLARAASRQSEFSIRTALGAGRWRMVRQLLTE
                     SVLLSVLGGALGLLIALWGVDALVALSPPNMLGTEKIGLSLPVLLFTFLISLITGVVF
                     GLVPALEVTRLNLNETLKETGKSNMSSRRTRRLSSGFVVAQVALALVLLIGSTLMIKS
                     FLRLQAVDPGFKTDNLLTLRVTVPAAKYPEDNQIVAFHRQALERLKSLPDVRSVGAVS
                     ALPFGGNLGARTSFSIEGRPVPRPGEELSTDVRVTDENYFQTIGIPVLSGRGFTEQEA
                     KEDRRTIIINETMARQHFPGQNPLGQYLRVQMMPDPQPMEIVGVVADAKYKTLEAPPH
                     PMVYWTHPQLTYSEMTLIVRTNGDPMNLAAAVRREIQAIDKDQPVADVRTMQSWIDEL
                     TARSRFGTLLLTIFAALALVLASIGIYGVMSYSVTQRTHELGIRIALGAKTLDVLKLI
                     LGRGLVLTLIGIALGLAASIVLTRFMSSLLFGISATDPITFGGLSLLLTAVALLACYV
                     PTRRAMRVDPMVALRNE"
     gene            13125..15566
                     /locus_tag="VGB76_04055"
     CDS             13125..15566
                     /locus_tag="VGB76_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098111.1"
                     /translation="MNTFWQDIRYALRMLLKSPGFTLVTVLALALGIGATTAIFSVVN
                     TVLLRPLPYPNSERVVYMGEWSQQIPEMSVSYPNFMDWRDQNQVFEQIAAFRNSNYVL
                     TDVGEPERLDGRQVSAAFFPVLGVNPAIGRNFTPEEDKPGANPVALISHGFWQRRLGS
                     DPSVVNKPLLLNGESYTVIGVLPQTFEWQSPVDVFVTVGLRSDRLMDRDNHPGMYVLG
                     LLKPGVSVEQARTEMKAIAARLADQHPETNAGNSVALQSLQERAVEDIRVALWVLFWA
                     VIFVLLIACANVANLLLARAASRSKEIAIRTALGAGRLRLIRQLLTESLLLSLLGGLC
                     GLLFAMWGIDALLSAIPDDVPRLLTANIGLDRWVLLFTFGVSVLTGLLFGLAPALQAS
                     KTNLNDSLKEGGRSGSAGGSHQRVRNMLVVSEVALSLLLLVGAGLLIQSFLRLQKTDL
                     GFDPANVLTLRVPLPEARYKENAQVASFWDELQNRVRVLPGVESVGLTRGLPMNGGIE
                     SGFTIEGREVANIKDLPIAVNLFTSANYFQTMNIPLLSGRYFTEQDTKDSQPVVLVDE
                     MLAARFFPNTDAVGKRLKLGGADSESPWMQIVGVVKHVKHYSPNEEPRVEVYTHYRQV
                     PERFFAAATRSMWVAVKTRTADPTSLTTAIRNEVQQIDKDQPISNVDKMENIVAATVA
                     PQKFATWLLGIFAAVAMVLAAIGIYGVMAYSVTQRTHEIGIRMALGAGQRDVLRMVVG
                     QGMKLALVGVALGLAGAFAATRLMSTLLFGVSATDPLTYSSVALLLGAVALFACLIPA
                     HKATKVDPMIALRYE"
BASE COUNT         3123 a         4800 c         4813 g         2884 t
ORIGIN      
        1 tgcgcgccgc accgtcgggc tggtgcgcgc gcggttagac gaatagggat gagggatgaa
       61 ggataaggga tgtgggatgc gggatgaggg aggaatccga gaccgcattc acgcgagtga
      121 gtgctttctc tgattcatcc ctcatccctc atccctcatc ccttatcctt catccctcat
      181 ccctattctt ctaaccgcgc gcgcaccagc cagacggtgc ggcgcgcatc agtctcggag
      241 acgtaggcga cgaagagttg ctggcctgtt ttcgttgcca caggtagctc gcccgcgctt
      301 gccagcgtat cggctggcgg agtttccacg ccgtccttga agtgcgtcgc ggcgatgcgc
      361 gtggccgcgc cctcgccgcg ttcttcccag accgccatca ggtgatcgcc gccttgaccc
      421 gccagtaaaa cgggcgtgcc gcgcccgctg ctgcgtgcaa aggcacgacg cggcgcgaag
      481 gtgcgcccgc cgtcgagcga ttgcgtttga tagaggccgg gcgttcccgc ttcgccagcc
      541 gtgtaccaga gcacggagag cgtcccgtcg tttgtgaggg agagagccgc gccggagaca
      601 gggcacccgg cgatctgcca cttgtcgtcg ttgacaatcg caggcgacga aaaggtgcgg
      661 cctccgtccg tcgaagcggc gacggcgatg tgccggaact cgcccggcaa cacctgccgc
      721 caactcgcat agacgcgtcc gtccgcgcct gccgcgagcg atgtcttgca gcacgggcac
      781 acttccgatg cgatgcgacg gttggcttcg aaggtgcgcc cgccatccgt ggaggcggca
      841 aagaaaattt cccggttgct ctccttgtgc ctgtgcgtgg agttgggctt cggcggggga
      901 ggcggggcga cgtttcgttc gtcgagccac gccatgtaga tgcgcccgtc cggggctgcg
      961 gccagtgagt gcatgccgtg agccgcaggc cgctcgtcgt cgttaacttt gacgggagac
     1021 gcgaaactgc gcccgccatc gcgcgaggcc gagagataga gcgtgctggc atgtttcgcc
     1081 cccggcacgc gcgccgtcca accgacatag accgtcgagt ccggcgcaac tgcgattgtc
     1141 ggcgggtcgc catgccacgc cgtcgccgcg cccgcctgtg gattaacgcg cacgggcgca
     1201 ccgagcggct tcgcttcgcc gtcgagatgc gcgagccaca cgtccgcctc gccgcccgcg
     1261 cgatgctcga cccacgcgac aaagaccgac ccgtcgcccg ccgccgcgag cgcaggctcc
     1321 gccgcatccg tatccttaac ggaaacaagg acaggctcga cagaggttat ccgtcgaacg
     1381 tccttcgcgg agttcggcgt cgttgaggac gcagcagaag acgcctgttc cacgcgtcgc
     1441 gcgggctttg agcaaccgac gagcagcagt aagagcagcg aggcgcaggc cgccgcgatc
     1501 cgttttttta tcatggcatc tctcccgtta aaataaaggg tcgctcaagt cggacggagc
     1561 ttgtcgcggg tgaacggcag ccgccggggt tgcaccgatt ttacacctcc ccagcgcgag
     1621 ccagctaacg gaagtctctc tttcacgacc gcaccgcgtc cgagtttcgt gaacggctga
     1681 actcaggggc gtatcggcga acagttcgcg tacgtgcgaa cgactctcgc cgcttgtctt
     1741 ttaccgtgcg gagtgaagta tcattcgtgc gagagacgat gaagcaacag gttaaaactg
     1801 aagaacagca gcagcagggc gcgccggagc agaaggcgga agcgcacccg agccactacg
     1861 aacctgccaa gcaggatttg aagtcggagc gcgagttgcg cgccgaagtc gcggctgcgt
     1921 ccgaagccgg gtcgcaaccc gatgccgcgc aggagttaaa ggagcctgcg cacgcgtcgc
     1981 gccgcgcgca gccgcccgaa gatttcacgg gcatcaaaat ttcgcacgcg gagaaagccg
     2041 ccggggggct tccggccatc atgtcggcca tgaggcattc ttggtcggag atgggcgcga
     2101 cgcgcacgct caagactctc acgcgcatca accagaaggg cggcttcgat tgccccggct
     2161 gcgcgtggcc tgaacccgac ggcgaacgca cgcacttcga gttttgtgaa aacggagcca
     2221 agcacgtcgc cgatgaagcg acgacgaaac gcatcacgcc ggacttcttc cgcaagtgga
     2281 gcctcaccga actctcgcgc cagtccgaca tgtggctgaa cgatcaggga cgcctcacgc
     2341 atcccatgtt gctcaggcgc ggcacagact tttacgaacc cgtcagttgg gatgcggcct
     2401 tcgccctcat cgccggagag ttgcacgcgc tcgcttcgcc cgacgaagcc gtcttctaca
     2461 cttccgggcg caccagcaac gaggcggcgt ttctctatca actcttcgta cgcaaattcg
     2521 gcacgaataa tctgcccgac tgttcgaaca tgtgtcatga atcgagcggc tcggctctga
     2581 ccgaaagcat cggcgtcggc aaaggcacgg tcacgctcga agacttcgca gaggcggaag
     2641 cgatcttcgt catcgggcag aatcccggaa cgaatcaccc gcgcatgctc tcgactttgc
     2701 aggccgccaa acgacgcggc tgtcgcatcg tccacatcaa ccctctgccc gaagccggaa
     2761 tgtcgcgttt caaacacccg caggaggtct tcgacacgct gattggcagc ggcacgcaac
     2821 tggccgacct cttcctgcaa gttcgcatca acggcgatgt cgcgctcctg caaggcatcg
     2881 ccaaagaggt tttggaagcg gagtcgcagc gcgcgggcga agtgctcgac catgacttca
     2941 tcgcggcgca caccacaggc ttcgaggcgt tcgcagcatc gctcggcaaa gtggtctggg
     3001 aagagatcac ggagagttgc ggcatcgggc gcgaagagat acgcgaggcc gcgcgtatct
     3061 ttatcgaatc gaagcgcacg atcttctgct gggcgatggg actcacgcag cacaagaacg
     3121 cggtggcgaa cattcaggag atcgtcaacc tgctactgct gcgcgggcag atcggccaga
     3181 cgggcgcggg cgtgtgtccc gtgcgcggcc attccaacgt gcagggcgac cgcacgatgg
     3241 gcatctggga acgcccgacc gcagccttcc tcgacgcgct cggcagagag ttcgacttcg
     3301 cgccgccgcg cgcacacggc ttcgacacgg tggaagcgat taaggcgatg cacgcgggga
     3361 aggcaaagct cttctttgcg atgggcggca attttctgtc ggccacgccc gacacggagt
     3421 acacggcgga agccctgcgc cgcctgcgtc tcaccgtgca agtctccacc aaactcaacc
     3481 gcgcgcacct catcacgggc gagcaggctc tcatcctgcc gtgtctcggt cgcaccgaaa
     3541 ttgacaggca ggcgacgggc gagcagtttg tcagtgcgga aaattcgatg ggcgtcgtgc
     3601 agtcttcgcg cggaaacctc gaaccggcat ccgcagaact cctgagcgaa gtggcaatcg
     3661 tggcgcgact cgcggatgcg actctcggcg gcacgcgccg cgacaggatc aactggctcg
     3721 aactcgccgc cgattacgac ctcatacgcg aacgcatctc gcgcgtcgtc gtcggtttcg
     3781 agaattacaa tgagcgcgtg cgccgtcccg gcggcttcta cctgccgaat ctcgcgcgcg
     3841 cacgcgaatg gaaaaccgct tcgggcaaag cgcagtttac cgtccacgaa ctgccgcacc
     3901 acgcgctcgc gcccggacag tttttgatga tgaccattcg cagccacgat caattcaaca
     3961 cgaccatcta cggactcgac gaccgttatc gcggcatccg caacgaacgg cgcgtcgtcc
     4021 tgcttaaccc ggaagacatc agcgaggcgg gactcgatgc aggctcaaac gtcgatctcg
     4081 tcagccactt cgaaggcgaa gaacgcatcg cgcgccgctt catcgtcgtg ccttacagca
     4141 tcccgcgccg ctcagccgca acctacttcc cggagacaaa cgtgctcgtc cctctcggca
     4201 gcgtcgccga caagagcaac acgcccgcct ccaagtcagt cgtcatctcc ctgcgcccct
     4261 gcgtcgccgc aacttaactt tggtatgtat gtagctcctc gcagcgagtg ttgaatgcct
     4321 ttggcgcatg cgggttattg cgggcgtgaa ccggccaccc ttattttttg cggcttcaaa
     4381 aatcctatac ttgaggagat agaaagatgg cgacatcgag gctcggtaca tactcgctct
     4441 ttctggctgt catcctgtgg gggacagtgc tcggcgggat tgtctattca catctcgttt
     4501 actttccggt ctatctctcc gctctgcctg cctcgtccgt cgtggtcaac gggacgtatg
     4561 ggctgcacga agcgatattc tggataactg ttcacccgct gctcatcctc tcgctcatcc
     4621 tcgctctcgt cttgaactgg aaaaacaggt cgcggcgcaa gctcatcttg atcagttgcg
     4681 cgatctactt tctggtgctt gtcatcacgc aaatctattt catccccgaa ttgatagcct
     4741 tcgaacaaag tcctgcctca aacgtctcag cctcggaatg gtttgcgcgc gggcagcgtt
     4801 ggcagcgatt gagctggctc cggggcgtgg tcatgtatgc cgccatcttg cctttactgc
     4861 tggcgttgac cagagcggct cccgatgcgg gagcgcgtgc gtttagcgcg acagacgcca
     4921 gagcatgaga cagccgatgg tgaggccgat gaggacgacg gcgcggcgca cgaacgtcct
     4981 gccgagtttg cgcgctatct ccgcaccgta gtaaccgccc atgatcgcgc ccgccgccat
     5041 gagcagggct ggcgtccagc gcacgaggcc ggagagggcg aaggccacga ccgcgacgct
     5101 gttcaaagtt agtccgagaa aattcttgag gccgttggcg cggtggatgt cggagatgcc
     5161 gagcagcccg agcgcggcga gcatcagaat gccgttgccc gcgccgaagt agccgccgta
     5221 gactgcggcg caaaactgaa agaggatcgc gcccgtccac cagcctctct gcctgcctgc
     5281 ggcgggcgtc gcctcaaggc gcagccagcg cgtgatcggt tcctgcgcca tgaagagaag
     5341 cgtggcgaag aagatgagga aagggacgag gcgggcgaaa gtttcggatg gcgtccggat
     5401 cagcaacgct gcgccgaccg cgccgccgaa gaaactcgtg attccgagac ggatgagcag
     5461 ggggcgactt ttttcgagtt cgcgccggaa gccgaacagc ccgccgacga gtcccggcca
     5521 gagcgcgacg gtgctggtcg cgttggcctc tttcgggtcg agtccgagcc agatgagcgt
     5581 ggggaaagtg aggagcgtgc cgccgcccgc aatggaattg atcatccctg cgatgaacgc
     5641 ggaggcgaac aggacgactc cttgaagaag agaaaagccc atctggtttg ccacaaaaat
     5701 gagactcgat agcgttaaac tgaagagact atacatgatg aatacgcgcg cggtcagtcg
     5761 cgttcggatg gacgcgcgac cggcgcacaa ttgacgagga gttaggcatg gcaatcgcga
     5821 gtgtgaatcc ggcgacggga gagaagctgc gcgagttcgc gccgctcacc gaagcggaga
     5881 tcgaagaaaa actggagcgc gccgcccgcg cctttcaaac gcacaggcgc acgagtttcg
     5941 ctgaacgcgc cgaacgtatg ctgcgcgcgg cggatatttt ggagacggag aaggaacgct
     6001 tcgcgcgttt gatgacgacc gaaatgggca agcccgtgcg cggggcgcgc gaagaggtgg
     6061 cgaagtgcgc gctcgtctgt cgttactacg cggagcacgc cggaagcttt ctcgcagatg
     6121 agacggtgga gacgagtgcg acgcgctcat tcgttcatta cgaaccgctc ggcgcggtgc
     6181 tcgccgtcat gccctggaac tttcctttct ggcaggtctt tcgctttgcc gcgcccgcct
     6241 tgatggctgg taacacgggg ctgctcaagc acgcttcaaa cgtgccgcag tgcgcgctgg
     6301 cgattgaaga gattttcctg cgcgcgggct tccctgtggg cgtcttccag accttgctgc
     6361 tcaactccga tcaggttgcg cgcgtgatag acgatgcgcg cgtgcgtgcg gcgactttga
     6421 cgggcagcga acaggcgggc agacaggtgg cgagccgcgc cggaggcgaa ataaaaaaga
     6481 ccgtgctcga actcggcggc agcgacccct tcatcgtcat gccgagcgcg gatttcgaga
     6541 gcgcagtcaa gacggcggtt aaagcacgca cgatcaataa cggccagtcg tgcattgcgg
     6601 ccaaacgctt catcgtccac acggagattt acgaagagtt cgcgcgtcgc tttgtcgccg
     6661 aactggaggc tttgaaggtc ggcgacccct cggacgagac aacggaaata ggaccactcg
     6721 ccaccgaaag catcgtcgcg gaactcgacg agcaggtgcg aaagtcaatc gagacgggcg
     6781 cgcgcacgct caccggcggc gaacgcttgc agcgcgcggg caattactac gcgcccaccg
     6841 tgctcacaga tattccgaaa gattctccgg cctacgggga agagtttttc ggtcccgtcg
     6901 ccctgctgtt tcgcgtgggt tcgattgacg aagcgattgc gctggcgaac gacacgacgt
     6961 tcggacttgg cgcgagcgcg tggacgcgtg cagaagatga gcgcgcgcgt tttgttgacg
     7021 aactcgaagc gggcgcggtc ttcatcaacg cgatggtggc atccgacccg cgcctgccct
     7081 tcggcggcgt caagcgttcg ggctacgggc gcgagttggg cgcgttcggc atacgcgaat
     7141 tcgtcaacat caagaccgtc tggctctcgg acgacggcgg cgagcggcgc accgagacgg
     7201 agtaagcgac aaactatctc ttacaaaacg ttgaactctc gatccatttg tttgtaagcc
     7261 gcgcgcttcg cgttgttcac tttcggacaa cgcgcgtccc gcaagtggga cgagaagagc
     7321 gcgcggctct acgtctcttc acataaaagc ctgcaaaact gattcaacga agtttccgcg
     7381 cgaagtggca cgccgcttcc atagtatctg cctgatcttc cggcgctggt tgaaggacgt
     7441 gctgccagag cgtctccatt cctacctcaa catttaagaa acgcgacggc ctcgccgtgc
     7501 gttaggtgaa acatgattcg tttgcgcaac gtcgaaaaag tttacgagca gggtgtgaat
     7561 agaacctacg tcttgcggcg cgtggatatg gacgtgcgcg agggcgagtt cgtctccatc
     7621 atggggcctt cgggcgcggg caagtcaact ttgctctcca tcctcggcat gttcgatagc
     7681 gcgtggacgg gcgaatacta tttctgggac gcggcagttc ataagttgaa caagaaagag
     7741 cgcgccgagt tgaacaaacg ctacatcgga ttcgtctttc agagttatca cctgctcgat
     7801 cacatgacgg tctacgagaa tctggacatg ccgctctctt acaaggatgt caaaaaatcc
     7861 gagcggcaat ccatcgtcgg cgatgtgctc gaccgtttcc agatcgtggg gaagaaggat
     7921 ttgtatccga accagttgtc gggcgggcag caacagttgg tggccgtcgc ccgcgccctc
     7981 atctcgaatc cgaaactgct cctcgcggac gagccgacgg ggaatcttca ttcgaatcag
     8041 ggacgcgaga tcatggaact cttcaagcgt ctgaacgaag aaggcacgac cattattcag
     8101 gtcacgcact cggaacagaa cgcggcctac ggccatcgca tcgtgcaact cgcagacggc
     8161 tgggtggtga aggaagagaa ataatttcgg gttgcggaat gctgactgga aaacaggaag
     8221 ccggtagtta aacgcctttg cttatcaagc ggcactcctt tgccggacgc tccgaaatcg
     8281 aaatggtttt gttgcgaaag tgggaaacgg tggttgcgac gcgtcgccgt aaaagtcgga
     8341 aagacggcaa aaacagtgcg aaatagagtt tttgcaggtg gcacgggttt cgcttaagcg
     8401 ggtgacggag gatggaaaat tttgtcaagt atggatgtcc ctcgcggtaa atcttcggct
     8461 cgcaagcggc gcgtgcgcgt cgctttgtat gccattatcg ccatcgtcgc tatcgcggcg
     8521 atcactttcg gcgtgtcgcg catgaagccc gccgcgccct cggtcgagcg ttcgaccgcg
     8581 ctcatcgaca cggtgaagcg cgggcagatg ctccggcagg tgcgcggcat cggcacgctc
     8641 gtgccgcagg acatacgcgt gatcgcggcg gccacggaag ggcgcgtcga gcgcgtgttc
     8701 gtccagccgg gtacggaagt cacggcgggc acggtgctca tcgaactcag caaccctgag
     8761 ttgcagcagg gcacggttga catcgagtat caggtgcgcg ccgccgaagc ggattacaac
     8821 aacatccgcg cgcgtcttga cagcgaacgc atgagccagc aggccgccgc cgccaccgtg
     8881 caggccgaat accagcaggc gaaacttcaa tccgacaccg acgaagaact cgccaaagac
     8941 ggtttgattc ccgccatcaa tctcaaactc tcgcgcgtgc gcgtggccga tctcgccaat
     9001 cgttacagcc tcgaaaagaa gcgtctggac ggcacggcga aatccgcgca ggcgcaactc
     9061 gcctcgcaac aggcgcgcat cgcccaactc aaggcgcaac tccaactgcg gcagacgcag
     9121 gtggcgacgc tgcgcgtgct ggccggaacg aacggcgtgc tgcaacagat gcaggtcgaa
     9181 gtcgggcagc aggtgcaacc gggaacgaac ctggcgcgcg tcgtcgaacc gcagaacctg
     9241 aaggccgaac tccgcatcgc agagacgcag gccaaagaca tcacgctcgg ccagcaagcc
     9301 agcattgaca cgcgcaacgg catcattccc ggccacgtcg tccgcattga tccggcgtcg
     9361 gagaacagca cggtgcgaat tgacgttgcg ctcgatgcgc cgctgccgca gggcgcgcgt
     9421 ccagacctga gcgttgacgg caccatcgaa ctcgaacgcc tcgacaacat tctctacgtt
     9481 tcccgccccg ccttcggaca ggcgcagagc accatcggca tgttcaaact cgaaacgggc
     9541 gggcagtttg ctacgcgcgt gcaggtcaag ctgggtcgca gttccgtcaa caccatcgaa
     9601 attctggaag gcttacagga aggggatcag gtgattctct cagacacttc gcagtgggac
     9661 gcctttaatc gcatacgact taactaagga aaggaagaag tgttgagtga tgagtgatga
     9721 gtgatgagtt aaaagacaaa gattttgttt taactcatca ctcatcattc atcactcatc
     9781 acttagaaaa caatgagcgc atcaaaacaa cctctgatcc gcctgcaaga tgtgaagaag
     9841 gttttcttca cggatgaagt ggagacgcac gccctcgccg gaacgcatct cgacatcgcc
     9901 aacggcgaat acgtctctat cacagggcct tccggctgcg gcaaatcaac tttgctctcc
     9961 atcctcggcc tcctcgattc tccgaccgaa ggcaagtact ggctgaacgg caaactcgtc
    10021 tccgatctct cgctcggcga acgcgcgcac atacgcaacc gcgaagtcgg gttcatcttt
    10081 caaagtttca atttgatcgg cgacttgagc gtctatgaaa acgtggaact gccgctcacc
    10141 tatcgcggca tgtcgaacgc agaccgcaag gcgcgcgtca cggatgctct ggagaaggtc
    10201 ggcatggcgc atcgcgcgaa acatctgccg agccaactct ccggcggtca gcagcagcgc
    10261 gtggccgtcg cccgcgccgt cgcgggtcaa ccgctgatcc tgctcgcgga cgagccgacg
    10321 ggtaacctcg attccaagaa cggcgaggcc gtgatggact tgctgcgcga actgcaccgg
    10381 ggcggcgcga cgatctgcat ggtcacgcac gatccgcgct acgcaacctt cgccgaccgc
    10441 gccatacatc tctttgacgg gcgcgtggtg aaagaagaga tggtcagttt caaggatgcg
    10501 gatgatgaag ccgcgcaggc cggacaagcc ggtacgcagg taggagtggc gtaatgggga
    10561 atctctggca ggacgttcgc tacggcttcc ggactttagt taaaagtccg ggtttcacct
    10621 tgatcgcgct catggcgctc gcgctcggca tcggcgcgaa caccgcgatc ttcagcgtcg
    10681 tcaacgcggt actgctgcgc gacttgcctt atcgcgaatc cgatcaggtg atgatcgtgt
    10741 gggagcaaaa tcgcgcgcgc ggcgggcgaa acaacgtcgc ttcgctggcg aactttttag
    10801 actggcaaca gcaggccaac agttttcagg agatggcagc cttctacgat gcggccttca
    10861 acctgacggg cgaaggcaac ccggaagaga ttccggcgca ggtgacgagc ggcaatctgc
    10921 tgacgatgct cggcacgcaa cccgcgctcg ggcaggtcta tacgaaggac gaggccgagc
    10981 cggggcgcga cgacgtggtg gtcatcagcc acggcctgtg gcagcgtcgc ttcggcggcg
    11041 cgggcgacat catcggcaaa aaaatcagtc tcaacgggca gaacgtgacc gttattggcg
    11101 tgatgccgca gggcttcaag tggttcatga aagaaggctc gcgcaccggt aaggccgcag
    11161 agatatggac gccgaccaac ttcgcttcgg tcttgaacgg cgcgacgaag gggcgcggtc
    11221 gcttcatcac cgtggccggt cggctcaagc cgggcgtcac gcgcgaacag gcgcaggccg
    11281 agatgtccac catcgccagc cgtctcgaac aacagcacgc acaattcaac accaacatgg
    11341 gcatccatgt cgtgcccgtg cgcgagcaac tggcgggcga gatcaagatc gcgctctacg
    11401 tgctgctcgg cgcggtcggg ttcgtgctgc tcatcgcgtg cgccaacgtc gccaacctga
    11461 tgctggcgcg ggcggcgagt cgccagagcg agttctccat acgcaccgcg ctcggcgcgg
    11521 gacgctggcg tatggtgagg cagttgttga ccgagagcgt gttgctgtcc gtgctgggcg
    11581 gcgcgctggg gttgctcatc gcgctgtggg gcgtggatgc gctcgtcgcg ctcagcccgc
    11641 ccaatatgct ggggacggag aagatcggcc tcagcctgcc cgtgctcctc ttcacctttc
    11701 tgatttcact aataacaggc gtggtcttcg gtctggtgcc tgcgcttgaa gtgacgcgtt
    11761 tgaatctcaa cgagactctc aaggagacgg gcaagagcaa catgagcagc cgtcgcaccc
    11821 ggcgcttgag cagtggcttc gtcgtggcgc aggtcgcgct cgcgctggtg ctgcttatcg
    11881 gctccactct gatgatcaag agtttcctcc gcctgcaagc ggttgacccc ggcttcaaga
    11941 ccgataacct gctcacgctg cgcgtgaccg tcccggcggc gaaatatccg gaggacaatc
    12001 agatcgtcgc cttccaccgg caggctcttg agcggctcaa atctctaccc gacgtgcgct
    12061 cggtcggggc tgtgagcgcg ctgcctttcg gcggcaacct cggcgcgcga acctctttca
    12121 gtatcgaagg gcgacccgtt ccgcgtccgg gcgaagaact ctcgaccgat gttcgcgtca
    12181 cggacgagaa ctattttcag acgataggaa ttccggtgct cagcggtcgc gggttcaccg
    12241 aacaggaggc gaaggaagac cggcgcacca tcatcattaa cgagacgatg gcgcggcaac
    12301 atttccccgg ccagaatcct ctgggtcaat atctcagggt gcagatgatg cccgacccac
    12361 aaccgatgga gatcgtgggc gtggtggccg acgcgaagta caagacgctg gaagccccgc
    12421 cgcacccgat ggtctactgg acgcacccgc aactcaccta ctcggagatg accttgatcg
    12481 tgcgcacgaa cggcgatccg atgaatctgg ccgccgccgt ccggcgcgag attcaggcga
    12541 ttgacaaaga ccagcccgtg gccgacgtgc ggacgatgca gagttggatc gacgagttga
    12601 cggcgcgcag ccgcttcgga actctattgc tcaccatctt cgccgcgctc gcgctcgtcc
    12661 tcgccagcat cggcatctac ggcgtgatgt cctattccgt cacgcagcgc acgcacgaac
    12721 tcggcatccg catcgcgctc ggcgcaaaaa ctctcgatgt gctcaaactg atcctcggac
    12781 gcggactcgt tctcaccctc atcgggatcg cactcggcct cgccgcctct attgtcttaa
    12841 cgcgcttcat gtcgagcctg ctgttcggca tcagcgcaac cgacccgatc accttcggcg
    12901 gcctatctct actcctgacc gcagtggcgt tgctcgcttg ctatgtgccg acgcgccgcg
    12961 cgatgagagt tgacccgatg gtcgcactcc gaaatgagta aggcagtggc gagtggcgtg
    13021 tgacaagtga caagttaaaa cagcgatcct gtctttgaat ttgtcttgtc tcttgctcgt
    13081 cactcgtcac acgccactcg tcactgttct agagaggtgc ccccatgaat acattctggc
    13141 aggacatacg ctacgctctg cgaatgctct tgaagagtcc gggctttacg ctggtgacgg
    13201 tgcttgcgct cgcgctcggc atcggcgcaa ccacggccat cttcagcgtc gtcaacacgg
    13261 tcttgctgcg cccgctgccc tatccgaaca gcgagcgcgt cgtctacatg ggggaatggt
    13321 cgcagcagat tccagagatg tccgtgtcgt atccgaactt catggactgg cgcgaccaga
    13381 atcaggtctt cgaacagatc gctgctttcc gcaattccaa ctatgtgctc acggacgtcg
    13441 gcgagccgga gcgactcgac gggcggcaag tttcggcggc cttctttccg gtgctcggcg
    13501 tgaatccggc catcgggcgc aacttcactc ccgaagagga caagcccggc gcaaaccccg
    13561 tcgcgctcat cagccacggc ttctggcaga gacgtttggg cagcgatcct tccgtcgtca
    13621 acaaaccttt gttgctgaac ggcgagagct acacggtcat cggcgtcttg ccgcagactt
    13681 ttgaatggca gtcgcccgtc gatgtgttcg tcacggtcgg cctgcgctcg gatcgtttga
    13741 tggaccgcga caaccatccg ggcatgtacg tgctcggatt gttgaagccc ggcgtctccg
    13801 tggaacaggc gcgcacggag atgaaggcca tcgccgcgcg cctcgcggat cagcatccgg
    13861 agacgaacgc gggcaacagt gtcgcgctgc aatcgttgca ggagcgggcg gtggaggata
    13921 tacgcgtcgc cctgtgggtc ttgttctggg cggtcatctt cgtgctgctc atcgcgtgcg
    13981 cgaacgtcgc caacctgctg ctggcgcgcg ccgcttcgcg ttcgaaagag atcgccatcc
    14041 gcaccgcgct cggcgcgggt cgcctgcgcc tcatccgtca attgctcacc gagagcctgc
    14101 tgctctcgct gctcggcgga ctatgcggcc tgctcttcgc catgtggggc atcgacgcgc
    14161 tcctgtccgc catccccgac gacgtgccgc gtctcctcac cgccaacatc ggcctcgacc
    14221 ggtgggtgct ccttttcacc ttcggcgtct ccgtgctgac gggtctgctt ttcggactcg
    14281 cgcccgcgct gcaagcctcg aaaaccaatc tgaatgattc gttgaaggaa ggcgggcgca
    14341 gcggttcggc gggcggaagc caccagcgcg tgcgcaacat gctcgtcgtc tccgaagtcg
    14401 ccctgtcgtt gctgctcctc gtcggcgcgg ggctgctcat acagagtttc ctgcgcttgc
    14461 agaagacaga cctcggcttc gaccccgcga acgtcctgac gctgcgcgtg ccgctgccgg
    14521 aagcgcgcta caaagagaac gcacaggtgg cgagcttttg ggacgaactg caaaaccgcg
    14581 tgcgcgtcct gccgggcgtc gagagcgtcg ggctgacacg cgggttgccg atgaacggcg
    14641 gcatcgagtc cggcttcacg attgaaggtc gggaggtggc gaacatcaaa gacctgccga
    14701 ttgccgtcaa cctcttcacc agcgcaaatt atttccagac gatgaacatc ccgctcctca
    14761 gcggtcgcta tttcaccgaa caggatacga aagactcgca gcccgtagtc ctcgtcgatg
    14821 agatgctcgc cgcgcgtttc ttcccgaaca cggatgccgt gggcaaacgg ctcaaactgg
    14881 gcggcgcgga tagtgaatct ccgtggatgc agatcgtcgg cgtcgtcaaa cacgtcaagc
    14941 attacagccc caacgaagaa ccgcgcgtag aggtttacac gcactacaga caggtgccgg
    15001 aacggttctt cgccgccgcc acgcgcagca tgtgggtagc cgtcaagacg cgcaccgccg
    15061 acccgacgag tttaaccacc gccattcgca acgaggtgca gcagatcgac aaggatcagc
    15121 cgatctcgaa cgtggacaag atggagaaca tcgtcgcggc gaccgtggct ccgcagaagt
    15181 ttgcgacgtg gctcctcggc atcttcgcgg cggtggcgat ggtgctcgcg gccatcggca
    15241 tctacggcgt gatggcctat tccgtcacgc agcgcacgca cgagatcggc atccgcatgg
    15301 cgctcggcgc gggacaaagg gacgtgctca ggatggtggt cgggcagggc atgaaactcg
    15361 cgctcgtcgg agttgcgctc ggcctcgccg gagcgtttgc ggcgacgcgc ctcatgtcca
    15421 cactgctctt cggagtcagc gcgaccgacc cgctgactta tagcagcgtc gccctgctgc
    15481 tcggcgccgt cgccctcttc gcctgcctca ttcccgcgca caaggcgacg aaagttgacc
    15541 cgatgatcgc gctcagatac gaataagaga gtgacaagtg acaggtgacg agtgacaagt
    15601 taaaaccaaa aaacgtgctg
//
LOCUS       DASWNS010000119         3609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_160041, whole genome shotgun sequence.
ACCESSION   DASWNS010000119 DASWNS010000000
VERSION     DASWNS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160041"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..2138
                     /locus_tag="VGB76_04060"
     CDS             138..2138
                     /locus_tag="VGB76_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY0098112.1"
                     /translation="MSHLCQLQTARAASRLVAVCILFSLTFAPANVLRGQPSSRLKAT
                     LSQGVQVAAKAGDSKSAVQSLAFKPYARRASREALKWADAELRRMTLDEKIGQLISVG
                     VNATFLNQDSEAFRALRRQVEQNHIGGIILFRGAVYESTHLVNRMQRLARRPLLISAD
                     LEAGSGMRFDDTINFPWNMAVGATGNPEYARRQGEVTAREARAMGVQQIFAPVVDVNN
                     NAANPVINVRSYGEDPQAVARMAAAFTEGAQAGGVIATAKHFPGHGDTAIDSHRGLPI
                     IDVGRARLDAVELVPFRATINAGVGSVMIAHIGLPQIDPTTVSPLPRTEPRRGGYADS
                     EVLVENARLPATLSPAVVGGMLRRDLAFDGIIVTDALDMSGLTIYFAPGEAAVRAVEA
                     GADMLLKPSDPDAVVRGLREAVAQGRLTERRIEESARRLLAAKYDLGLQRQRIAPLEG
                     IDRVVSNEAALKLAQEIAEHAVTLVRDERRLLPLSNLAAGSRIFNLAITNGEDRLWVG
                     NPFAGALGRGGHKVESAVLDERSTEKEVDKILDKTKEADLIVISMYGRVRTGQSNSGA
                     LPEAGTRALRKLIERKSPLVGISFGNPYLLQSFPDLKTYMVAYGDMPSLQRAAARALL
                     GEIDITGKLPITLPSLYARGTGIQLKAQPRTPAEGRAANNTK"
     gene            2203..>3609
                     /locus_tag="VGB76_04065"
     CDS             2203..>3609
                     /locus_tag="VGB76_04065"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0098113.1"
                     /translation="MQKANSRYSMPLVALYSLRRRAAPLLLVLFVAQSFAANLPVVAP
                     ASVGMSAERLGRIDAAVGEAIARRETPGAVVLAARRGRVVWRKAYGARAVEPARELMT
                     PDTIFDAASLTKVVATATSIMILVERGQVRLSDPVSRYLPEVKDGGRERVTIEQLLTH
                     RAGYAPDFDLGERWTGYDEALKRLASERLQNAAGTRFVYSDIGFIALGEVVKRVSGET
                     LDLFARKNIFEPLRMHDTGFNPRAALRTRIAPTERRRAQAAYLGGKPESVGAEGERWL
                     RGEVHDPTAFRMAGVAGHAGLFSTADDLAIYCQMILNGGTYGGVRILSPLGVATMTRP
                     RAVTDEGGARGLGWDIASSFSGNRGDLFPLGSFGHTGFTGTSLWLDPASETFVVFLSN
                     RVHPDGKGDVGALRGRVASIVASSLMDATLEQARLESSRFASETLLSLARLTGTNTQA
                     NAASNTGATADAEVLNGID"
BASE COUNT          632 a         1117 c         1219 g          641 t
ORIGIN      
        1 gcttcgcctt cgcgcccgca tccgccgcgc aaagcgacga actcgctccc ggcggcgcaa
       61 tggaaaatct ctagttagcc tcgacatcgg ttggacggcg aaatcagttg acctttaaaa
      121 gagatcgaat taaccacatg agccatcttt gccaacttca aactgcgcgt gcggcttcgc
      181 gtctggtcgc cgtgtgcata cttttttcgt tgacgttcgc gcctgccaat gtcctgcgcg
      241 ggcagccgtc ttcgcgtttg aaggcaacgc tgtcgcaggg cgtacaagtc gctgccaaag
      301 cgggcgattc caaatctgcc gtgcagagtc tcgccttcaa gccatacgcg cggcgcgcct
      361 cgcgtgaggc gttgaaatgg gcggacgcgg aactgcgccg gatgacgctc gacgaaaaga
      421 tcggtcaact gatctcggtc ggcgtcaacg ccacttttct gaatcaggat agcgaagcct
      481 tccgcgcgct gcgccgtcag gtggaacaga atcacatcgg cggcatcatc ctgtttcgcg
      541 gcgcggtcta tgaatccacg catctcgtca accgtatgca gcgattggcg cgtcgcccgc
      601 tgctcatctc cgccgacctc gaagccggtt cggggatgcg cttcgacgat acgatcaact
      661 ttccgtggaa catggccgtc ggggcgacgg gcaatcctga gtacgcgcgg cggcagggcg
      721 aggtgacggc gcgcgaggcg cgtgcgatgg gcgtgcagca gattttcgcc cccgtcgtag
      781 atgtcaacaa caacgcggcg aaccctgtga tcaacgtgcg ctcttacggc gaagacccgc
      841 aggccgtggc gcgcatggcc gccgctttca cggagggcgc gcaggcgggc ggcgtgatcg
      901 cgaccgccaa acatttcccc ggccacggcg acacggccat tgattcgcat cgcgggcttc
      961 cgatcataga tgtcgggcgc gcgcggctcg acgccgtgga actcgtccct tttcgcgcga
     1021 cgattaacgc gggcgtcggc tccgtgatga tcgcgcacat cggtctgccg caaattgacc
     1081 cgaccacggt tagcccgctt ccgcgcacgg aaccgaggcg cgggggctac gccgacagcg
     1141 aagtgctcgt cgaaaacgcg aggcttccgg ccacgctctc gcccgcagtc gtcggcggga
     1201 tgttgcgccg cgatctcgcc ttcgacggca tcatcgtgac ggacgcgctc gacatgagcg
     1261 gcctgacgat ctacttcgcg ccgggcgagg cggcggtgcg cgccgtggag gcgggcgcgg
     1321 atatgctgtt gaaaccttcc gacccggatg cggtcgtgcg cggcctgcgc gaagccgtgg
     1381 cgcagggtcg tctgacggag cggcggatcg aggagtcggc gcgtcgtctg ctggcggcga
     1441 agtatgacct cggcctccaa cggcagcgca tcgcgccgct ggagggaatt gatcgcgtcg
     1501 tctcgaacga ggccgcgttg aagctggcgc aggagatcgc ggagcacgcg gtcacgctcg
     1561 tgcgcgacga gaggcgtctg ctccccttga gcaatctggc ggcggggtcg cgcatcttta
     1621 atctggcgat cacgaacggg gaagatcgtc tctgggtggg gaatccgttt gcgggcgcgc
     1681 tcggtcgcgg cggacataaa gttgagtcgg cggtgctcga cgaacgctcg acggaaaaag
     1741 aggtcgataa aattctcgac aagacgaaag aggccgacct gatcgtcatc tcgatgtacg
     1801 ggcgcgtgcg aacggggcag tcgaacagcg gagcgttgcc ggaagcgggc acgcgcgcgc
     1861 tcaggaagtt gatcgaacgc aaaagcccgc tcgtcggcat cagcttcggc aatccatact
     1921 tgttgcagag ttttcccgat ctcaagactt acatggtggc ttacggcgac atgccgagtt
     1981 tgcaacgcgc cgccgcccgc gccctcctcg gcgagataga catcacaggt aaactcccga
     2041 tcactctgcc ctcgctctac gcgcgcggca caggcattca attgaaggcg cagccgcgca
     2101 cccctgccga agggcgcgcc gccaacaaca cgaagtagaa gcagcgaatt caaccgtaga
     2161 aaatatatgc ccgctcgtca cactgttatt caacgccgcg tcatgcagaa agcaaacagc
     2221 cgttattcca tgcctctcgt cgccctttat tccctgcgtc gccgcgccgc gccgctcctg
     2281 ctcgtgctct tcgtcgcgca gagtttcgcg gccaacctgc cggtcgtcgc gcccgcaagc
     2341 gtcgggatgt ctgcggaacg tttggggcgc attgatgcgg cggtggggga ggccatcgcg
     2401 cgcagggaga cgccgggggc ggtggtgctc gcggcgcgcc gggggcgtgt ggtgtggcgc
     2461 aaggcttacg gggcgcgcgc cgtcgaaccc gcgcgtgagt tgatgacgcc ggacacgatc
     2521 ttcgatgcgg cgagtttaac gaaggtcgtg gcgacggcga cgagcatcat gatcctcgtc
     2581 gagcgcgggc aggtgcgcct gagtgatccc gtgtcgcgtt acctgccgga agtgaaagac
     2641 ggcgggcgcg agcgcgtgac gattgagcaa ctcctgacgc atcgcgcggg ctacgccccg
     2701 gacttcgatc tcggcgagcg ttggacgggc tacgacgaag cgttgaagcg tctggcgagc
     2761 gagcgtctgc aaaatgcggc gggcacgcgt ttcgtctata gcgacatcgg gttcatcgcg
     2821 ctcggcgaag tggtgaagcg cgtgtcgggc gagacgctcg acctgtttgc gcgaaagaat
     2881 atctttgagc cgctgcgcat gcacgacacg ggtttcaacc cgcgcgccgc tttgcgcacg
     2941 cgcatcgcgc ccaccgagcg gcgacgcgcg caggccgcgt atcttggcgg caaacccgaa
     3001 agcgtgggcg cggaaggcga gcgttggctg cgcggcgaag tgcacgaccc gacggccttt
     3061 cgcatggcgg gcgtggccgg gcacgccgga ctcttctcga ccgcagatga tctagccatc
     3121 tactgccaga tgatcttgaa cggcggcacc tatggcggcg tgcgtatctt gagtccgctc
     3181 ggcgtggcga cgatgacgag gccgcgcgcc gtgacggatg aaggcggcgc acgcggcctc
     3241 ggttgggaca tcgcgagcag cttctctggg aatcgcggcg acctcttccc gttggggtct
     3301 ttcggccaca cgggctttac gggcacttcg ctctggctcg atccggcgag cgagacgttc
     3361 gtggtcttcc tgagcaaccg cgtgcaccct gacggcaagg gcgacgtggg cgcgttgcgc
     3421 gggcgggtcg cttcaatcgt cgcctcttcg ctcatggacg cgacgttgga acaggcgcgt
     3481 cttgagtcgt cgcgcttcgc gtcggagacg ttgttgagtc tggcgcgctt gacgggtacg
     3541 aacactcaag cgaacgccgc gagcaacaca ggcgcgacgg cggacgcaga agttttgaac
     3601 ggcatcgac
//
LOCUS       DASWNS010000120         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_393714, whole genome shotgun sequence.
ACCESSION   DASWNS010000120 DASWNS010000000
VERSION     DASWNS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393714"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /gene="ribH"
                     /locus_tag="VGB76_04070"
     CDS             complement(<1..383)
                     /gene="ribH"
                     /locus_tag="VGB76_04070"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR00114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY0098114.1"
                     /translation="MNAKGFRFALVSSRWNDFLTSRLVEGALDALERLGAAEKSVEHF
                     RVPGSFEIPLLASKLADSEKYDAVICLGTIIRGQTPHFDYIAGEVTKGIAQAGMESGI
                     PVVYGVVTADTLEQAIDRAGVKAGN"
     gene            complement(568..1380)
                     /locus_tag="VGB76_04075"
     CDS             complement(568..1380)
                     /locus_tag="VGB76_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0098115.1"
                     /translation="MKPDEQAAEVFVEERKVGDSGALVCVVRLNRPAKRNALTRAMLE
                     RLTEIFRTFAGRTDLRAVLLTGAGLEAFSAGTDIAELDGLDPAGALQAARRGQETCEA
                     IELCGVPVIAALNGIAAGGGCELALACHLRVAATHATFSLPEIKLGMIPAYGGTQRLA
                     RAVGHGRALAAMLAGESLSAEDALRLGLVNRVVAPAQLLPEADALAETLAQLAPLALR
                     ACLTAVTRGARLPLADGLALEAELFSKLFATEDVREGTRAFIEKRPPNFKGK"
     gene            complement(1377..>1914)
                     /locus_tag="VGB76_04080"
     CDS             complement(1377..>1914)
                     /locus_tag="VGB76_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098116.1"
                     /translation="KVETVLPRAGLYKLHSDFFPVGGTLQVLHRELATAGYRTTTHAR
                     AALVADTSFTKTVDGMKISLDWGTGELSAGAFVRLKYRLADAQTGAPVKDLEPYLGAW
                     GHTLILNADQSDYLHSHPTETLPEGVPRAALRGGPEVEFGAMFPEAGLYRIWTQFQRG
                     GRVATVSFTIQVRPVAAS"
BASE COUNT          343 a          581 c          641 g          349 t
ORIGIN      
        1 ttgttcccgg ccttcacgcc cgcgcgatca atcgcctgct ccagcgtgtc agccgtgacg
       61 acgccgtaga cgacgggaat tcccgattcc attccggcct gcgcgatgcc cttcgtcact
      121 tcgcccgcga tgtaatcgaa atgcggcgtc tgcccgcgaa tgatcgtgcc gagacagatc
      181 accgcatcat acttttccga gtcggcgagc ttgctcgcca gcagaggtat ctcgaacgac
      241 cccggcacgc gaaaatgttc gaccgacttt tccgccgcgc ccagacgctc caacgcatcg
      301 agcgcgcctt caaccagacg cgacgtgaga aagtcgttcc aacgactcga caccagcgca
      361 aagcgaaagc ccttagcgtt caactggcct tgatgtgtga cgggctgcaa ctgtttccgg
      421 cgactccaaa cctgttgaag attgattcga cagcagtata ggtaaaacga gtagcgagtg
      481 acaagcggcg ggacaataaa gtgatgagtg atgggtgatg agtgatgagt aaaaacaaaa
      541 aatctgtctt ttattactca tcactcatca cttgccttta aagttcggtg gtcgtttttc
      601 gatgaaggcg cgggtgcctt cgcgtacgtc ttcggtggcg aagagtttgg agaagagttc
      661 ggcttcgagc gcgaggccgt ctgcgagggg gaggcgtgcg ccgcgcgtga cggctgtgag
      721 gcaggcgcgc agggcgagcg gcgcgagttg cgcgagagtc tccgcgagcg cgtcggcctc
      781 gggcaaaagt tgtgcgggcg cgacgacgcg gttgacgagt ccgagccgca gcgcatcttc
      841 cgccgacaga ctttcgcccg cgagcatggc ggcgagagcg cgcccgtggc cgacggcgcg
      901 tgcgaggcgt tgcgtgccgc cgtaagcggg aatcatgccg agcttgattt cgggcagaga
      961 gaatgtcgcg tgcgtggcgg cgacgcggag atgacaggcg agcgcgagtt cgcaaccgcc
     1021 gcccgccgcg atgccgttga gggctgcgat gaccggcacg ccgcacagtt ctatcgcctc
     1081 gcaggtttcc tgcccgcgcc gcgccgcttg aagcgcgccc gccgggtcga gtccgtcaag
     1141 ctccgcgatg tccgtgcccg cgctgaaggc ttcaagcccc gcgcctgtga gcaggacggc
     1201 gcgcaagtct gtgcgtccgg cgaaggtgcg aaagatttcc gtgaggcgtt cgagcatcgc
     1261 gcgcgtgagg gcgttgcgct tcgcgggacg gttcaggcgc acgacgcaga cgagcgcgcc
     1321 gctgtcgccg accttgcgct cctcgacgaa gacctccgct gcctgttcgt ccggcttcac
     1381 gatgctgcga cagggcgcac ttgaatggtg aaggagacgg tggcgacgcg cccgccgcgc
     1441 tggaactgcg tccagatgcg atagagtcca gcttcgggaa acatcgcgcc gaactctact
     1501 tcgggaccac cgcgcagagc cgcgcgcggg acgccttccg gcagcgtctc ggtggggtgc
     1561 gaatggagat agtcggactg gtctgcgttc agaatcagcg tgtgccccca cgcgccgaga
     1621 tacggttcga ggtctttgac gggcgcgccc gtctgcgcgt ccgcgagcct gtatttgagc
     1681 cgtacgaatg ccccggcaga cagttcgcct gtgccccagt ccagactgat cttcatcccg
     1741 tcaactgtct ttgtgaacga agtgtcggcg acgagggcgg cgcgtgcgtg cgtcgtcgtg
     1801 cgataaccgg cggtcgccag ttcgcggtgc aacacctgga gcgtgccgcc gacggggaag
     1861 aagtcggaat ggagtttgta gagtccggcg cgcggcagca ccgtctcgac cttg
//
LOCUS       DASWNS010000121         3447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_367552, whole genome shotgun sequence.
ACCESSION   DASWNS010000121 DASWNS010000000
VERSION     DASWNS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367552"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /gene="rnpB"
                     /locus_tag="VGB76_04085"
     ncRNA           <1..476
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGB76_04085"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            574..870
                     /locus_tag="VGB76_04090"
     CDS             574..870
                     /locus_tag="VGB76_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098117.1"
                     /translation="MVNRKGVFIGAYVPNDLKETLRRRAAAEHRTLSQEITRILTEAV
                     HGKTLPPGAIDRRNPVSSQPRRRDTDPPERRRRDDVPYKGLDGDGNSSSTTGSD"
     gene            1037..1825
                     /locus_tag="VGB76_04095"
     CDS             1037..1825
                     /locus_tag="VGB76_04095"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0098118.1"
                     /translation="MNKQKILYALAGLLAGCAIGFFFANSVNLKEIEKLRGEVARARN
                     NAGTTQPQQGAGRSADGNASGAPGGEMPSEDEIRDLIAKADASRDDADFQRKVGQGVY
                     MYALSTGQPALMPEAVRLLKRAHEADPKNYETTVLLGNSLFALAQVTDASKFAEARVY
                     YNKALATKPDDTNVQTLLGMTYFFGQPSDPERAIREYRKSLAAEPRQEMALQSLAAAL
                     IATGAHAEAQKRIEELQAVNPANAALSNLRAQLAQAKNAAKEKD"
     gene            1822..2157
                     /locus_tag="VGB76_04100"
     CDS             1822..2157
                     /locus_tag="VGB76_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098119.1"
                     /translation="MKYLIIAIVLGALCTIIYLRLRPYINIARRVLGVVRQARSMSAG
                     QQTPDASMRQQKTATDERLVRCASCGTWTPASRAITLRATSSNFCSHACMERSAGVNS
                     TPRAAGNQR"
     gene            complement(2283..>3447)
                     /locus_tag="VGB76_04105"
     CDS             complement(2283..>3447)
                     /locus_tag="VGB76_04105"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0098120.1"
                     /translation="CISISVFLQVFLLPILGAIADYSNLKKRLLALFCYLGVAATCLM
                     FAVTGSLYIAGGLLFIIANLSFGAALVFYNAFLPEITTEDQRDKVSSRGFALGYLGGG
                     LLLALNLLLVQFAPTLGISTGMAVRLSLLSAGIWWGGFALVAFARLQTRQAERSLPPG
                     KTYLTIGFSQLRATFRELARLRQTLRYLVGYLFFNDGIQTVISMAGVFLAQELFVSRG
                     REVNESFLIGIFLLVQFLAFFGALLFERIANALGTKRAIMLSLAIWAAIVIYAYGFLQ
                     TTTQAWAMAAMIALVLGGSQALSRSLFSRMIPEGREASFFGLYEISERGTSWLGPLVF
                     GAIVGATNSYRQAILSLIVFFVVGLIILSLTDTDKAIHDAGNVLPEEVRHATA"
BASE COUNT          782 a         1027 c         1085 g          553 t
ORIGIN      
        1 ttccgtcagg gagcgccggg ggaggaaagt ccgaacacta cagggcagcg agccagctaa
       61 cggctgggcg cgtgggttaa aagggggctt tcagtagccg cgttttgacc agcgcgacga
      121 caagtgcaac agaaaacaga ccagcccggc tgcgttcgcc ctgcggggcg aaagagcggc
      181 gggtgatggg tgaaatggtg cggcctcgca acagaggctt aatgtaagag cgcaccagcg
      241 cgtgtggtga catgcgcggc taggcaaact cctcgcagtg caagaccaaa tagggaggcg
      301 tcaaacaggg ctgcccgccc gatgccgcgc gaacttctac gggagacgcg cgctacgacc
      361 tccgggtagg tcgctcgatc caaccggcga cggttgggct agatgaatgg tcgccgctct
      421 ccgtcccgtg taaacgggcg ggagtacaga attcggctta tagacctgtt ctcaaactgc
      481 atggccgctc acgtttaact cacacgaagc aaaggctttc aaagtgtcgc aagtgcgagt
      541 atccgaactt tccgtcgagg agcttttaag agcatggtga atcgcaaagg ggtcttcatt
      601 ggagcgtatg ttccgaacga tctgaaagaa actctgcgcc gccgcgccgc tgccgagcac
      661 cgcacgctct cgcaggagat cacgcgcatc ctgaccgaag cggtgcatgg caagacgctc
      721 cctcccggcg caattgaccg ccggaatccc gtctcttcac aaccgcgccg ccgcgacaca
      781 gacccgcccg aacgacgccg ccgcgacgac gtgccctaca aaggattgga cggcgacggc
      841 aacagcagca gcacaaccgg cagcgattaa ctcaaactca catcaactgc ggaagtctcc
      901 gtcttgaatc cggcgacgga gcgcggcgtc gaaaagcgtc gctaaagagc gtcggcggag
      961 tgcgtcgcct ttccccgtgc gttctgataa actcttttcc tgacttcaat tttcgtttcc
     1021 cgtaactcgc ttcgtcgtga acaagcaaaa gattctctac gccctagccg gactcctcgc
     1081 gggctgtgcc atcgggtttt tcttcgccaa ctccgtcaac ctgaaagaga tcgagaagtt
     1141 gcgcggagaa gtggcgcgcg cccgtaacaa tgcgggcacg acgcagccgc aacagggcgc
     1201 gggacggagc gcggacggca acgcgagcgg tgcgccgggc ggggagatgc cgtcggaaga
     1261 cgagatacgc gacctcatcg cgaaggccga cgccagccgc gacgatgcag actttcaacg
     1321 caaggtggga cagggcgtgt acatgtacgc cttgagcaca ggccagcccg cgttgatgcc
     1381 cgaggcggtg cgactcttga aacgcgcgca cgaagccgac ccgaaaaatt acgaaacgac
     1441 cgtgctcctc ggcaactcgc tctttgccct ggcgcaggtc acggacgcat cgaagtttgc
     1501 ggaggcgcgc gtctactaca ataaggctct ggcgacgaag ccggacgata cgaacgtgca
     1561 gacgttgctc ggcatgactt actttttcgg ccagccttcc gaccccgagc gcgccatcag
     1621 ggagtatcgc aagtcgctgg ccgcagagcc gcgccaagag atggctttgc aaagtttagc
     1681 ggcggctctt atcgccacgg gcgcgcacgc ggaggcgcaa aagcgcatcg aagaactgca
     1741 agcggtgaac ccggcgaacg ctgcgctctc gaatctgcgc gcgcaactcg cacaggccaa
     1801 aaacgccgcg aaggagaaag attgaagtac ctgatcatcg ccatcgtcct cggcgcgctc
     1861 tgcaccatca tctatctgcg cctgcgcccg tacatcaaca tcgcgcgccg cgtgctcggc
     1921 gtcgtacgcc aggcgcgcag catgagcgcg ggacaacaga cgccggatgc gtcgatgcgc
     1981 cagcagaaaa ctgctaccga tgagcgtctc gtgcgctgcg cctcttgcgg cacatggact
     2041 cccgcgtcgc gcgcgataac gctgcgcgcc acctcttcca acttctgctc gcacgcctgc
     2101 atggaacgct ccgccggggt gaacagcacg ccgagagcgg cgggcaatca acgctaaaag
     2161 attaagactc gccacagagg gcacaaaggg ggcgcagaga gagttgaatc ttctctgtgc
     2221 cctctctgtt tcccctgtgc ctgcggtgtt gaatgcttcg ggtcttatca agccgcgacg
     2281 gttcaggctg tcgcgtgccg gacttcttcg gggagcacgt tgcccgcgtc gtgtatggct
     2341 ttgtctgtgt ctgtgagtga gaggatgatg aggccgacga cgaagaagac gatcagggag
     2401 aggatcgctt ggcggtaaga gttcgtcgcg ccgacgattg cgccgaagac gagtggcccc
     2461 agccaagacg tgccgcgctc cgagatttca tagaggccga agaaagaggc ttcgcgccct
     2521 tccgggatca tgcgcgagaa gagcgaacgc gagagagcct gcgagccgcc gagcacaagc
     2581 gcgatcatcg ccgccatcgc ccacgcctga gtcgtcgtct gtaaaaaccc gtaagcgtag
     2641 atcacgatgg cagcccagat ggcgagcgac aacatgatcg cgcgcttcgt gccgagcgcg
     2701 ttcgcgatgc gctcgaagag cagtgcgccg aagaaggcga gaaactgcac cagcaaaaaa
     2761 atcccgatca gaaacgactc gttcacttcc cgcccgcgcg agacgaacaa ctcctgcgcg
     2821 agaaagaccc cggccatgct gatgacggtc tgaatgccgt cgttgaagaa gagataaccg
     2881 acgagataac gaagcgtctg ccgtaagcgc gcgagttcgc ggaaagtcgc tctgagttgg
     2941 ctgaagccga tggtgagata agtcttgccc ggcgggagtg aacgctccgc ctgccgcgtc
     3001 tgcaaacgcg cgaaagcgac gagcgcgaag ccgccccacc agattccggc ggagagcagg
     3061 gagaggcgta cggccatgcc tgtcgagatg ccgagcgtgg gcgcaaactg cacgagcagg
     3121 agattcaagg cgagcagcaa cccgccgccg agataaccga gcgcgaaacc gcgactcgaa
     3181 actttatcgc gctgatcctc cgtcgtaatc tccggcagaa acgcgttgta gaagacgaga
     3241 gccgcgccga agctcaggtt ggcgatgata aagaggaggc cgcccgcgat gtagaggctg
     3301 cccgtgacgg caaacatcag gcaggtggcg gccacgccga gataacagaa gagcgcgagc
     3361 aggcgtttct tcaagtttga ataatcggca atcgcgccga ggatgggcag caggaaaact
     3421 tggaggaaga cggagatgga gatgcag
//
LOCUS       DASWNS010000122        10461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_290299, whole genome shotgun sequence.
ACCESSION   DASWNS010000122 DASWNS010000000
VERSION     DASWNS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290299"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..2812)
                     /locus_tag="VGB76_04110"
     CDS             complement(188..2812)
                     /locus_tag="VGB76_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098121.1"
                     /translation="MWEKSKLWLRALFRREQMERELDRELRFHLEREIEENLRRGMSL
                     KEARDAAQRSFGGFERIKEECREVRGVGIFETFLQDLRYGVRMLFKHRGFTFVAVLTL
                     ALGIGANSAIFSVVNAVLLRPLPYKDPDQIVYLWERLPQGGEGSVSVPNLRDWQEQND
                     LFEQIAAYEQGNFSLLGSDLPERSAGASVTANFFDVLGVAPKQGRTFITGEDTAGRNR
                     VVILSDQLWQRNFGADPRILNREVLIGGENYMVVGVMPPGFQFPSRTTELWVPLVFTD
                     RQAGNRGNHAFSSIGRMKPGVTLEQAQEQMNVIARRLEQQYPDQQAKRGITVGRLQEE
                     IVKSVRPALLILLGAVGFVLLIACTNVANLLLARAAARRREIAIRTALGAGRARLVRQ
                     FLTESVLLSILGGLLGLVVAKWGIDALLSLTTGVLPRASEVGLDLRVLGFTLLLSLLT
                     GIIFGLAPALQTSKADVQDALKDGGTSGSNPRANWLRSILAVAQVASAVVLLVGAGLL
                     VKSFIRLQQVESGVRPENVLTMRVTLPAAKYQTTDAATNFYTQVLERVSALPGVEAAG
                     AINLLPVQRSGTNGNVQIVGDPPTPSGQGALVEYRTATPDYFRALGIPLVAGRFFEPA
                     AREQAPVAIVNETFVRVILNGRDALAKQIQSDGTPIQIVGVVRDVKQTGLTTPPRPEM
                     FLPYNSPFWAGMTQNMSLVVRASSDPTTLTQAIRREVLAVDPAQPVYNVLTMEQVLDE
                     SISRQRLNMMLLSIFAALAMLLSTIGIYSVMSYLVTQHTREIGIRMALGAQPKDVLKL
                     VLGQGLMLTVIGIVIGVAGALALTRLMETLLFGVQATDPLTFLGVSLLLTFVALLACY
                     IPARRAMRVDPMVALRYE"
     gene            complement(2827..3159)
                     /locus_tag="VGB76_04115"
     CDS             complement(2827..3159)
                     /locus_tag="VGB76_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0098122.1"
                     /translation="MAKQKTDLLQGTLDLLILKILALGATHGWDISQRIQQISQDVLR
                     VNQGSLYPALHRLEAQGWIGSEWGTSDNNRQAKYYKLTAAGRRQLAAETAMWERFSGA
                     ISLILKTT"
     gene            complement(3407..4630)
                     /locus_tag="VGB76_04120"
     CDS             complement(3407..4630)
                     /locus_tag="VGB76_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098123.1"
                     /translation="MNLLMIIRVAFRALVRNKMRAALTMLGIIIGVSAVIAMVSIGQG
                     AQASVQAQIESMGTNLLFVSAGAQNVGGVRSGAGDSGTNTLTVADLEAIRREVPSVSM
                     VTPSVNARAQIVVGNQNWNTSVQGVSEQFPEVRKWTVGSGAFFTDADVRASARVIVLG
                     QTIADNLFPGADPLGQSVRVMNLPFKVVGVMARKGQDPQGRDQDDTAFAPYTAVQKKL
                     LGNTRLQVAYVSAISQDATYTAQTQIAELLRQRHKLTAGAPDDFTVRNMTDVAEAANE
                     TNNIMTMLLGSIAGVSLLVGGIGIMNIMLVSVTERTREIGIRMAIGARSSAVRRQFLI
                     ESIVLSLTGGLIGILLGIAVSVTIPMLLGWPTLVSTTAIVGSVIFSAVVGISFGYYPA
                     RKAAALDPIDALRYE"
     gene            complement(4673..5494)
                     /locus_tag="VGB76_04125"
     CDS             complement(4673..5494)
                     /locus_tag="VGB76_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0098124.1"
                     /translation="MQATLEIKPQELKLDARLLARAKAAPPVIELEGIHKTYTMGDVE
                     VHALRGISLTIRKGEFVAIMGTSGSGKSTTMNIIGCLDRPTQGSYILDGEDVSTMSKD
                     ERADIRSRKLGFVFQGFNLLSRTSALENVELPMLYAGIETAERHSRSREALRAVGLAG
                     RENNHSNQLSGGQQQRVAIARSLVNRPALILADEPTGNLDSRTSIEVMEIFQKLNRER
                     GITLVLVTHEPDIAEYADRVVVFKDGRIKEDYPVENRRDASEELRLLPDVDQEED"
     gene            complement(5582..7300)
                     /locus_tag="VGB76_04130"
     CDS             complement(5582..7300)
                     /locus_tag="VGB76_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0098125.1"
                     /translation="MKWIKSNLSGRKRFVIALGVVALVAFAGFVLWGDRASAAEYMTG
                     KVERGNLRNSVTATGTLQAVTTVQVGSQASGTISALHADFNSMVRKGQVIAQLDPATA
                     QAQVEQARANLVQARAALQQAQAGVAQSRAGVSQSRAVVTDAQAKALAARSTVQNQQA
                     GVYGAQANLAVLKAQMDDAASFLRQQESLASSGVIARREVETAQTSYKTAEARYQQAA
                     AQVQQAQLSAQSASSSGVVQSQAGIQQAQAGVQQAQAQAQNAAAQVQNAAAGVQQAQA
                     ALQLAEINLSQTTIRSPIDGVVVSRDVDIGQTVAASLSAPTLFTIANDLTQMQVMANI
                     DQADIGLVEQAKSVSFTVDAFPGMEFTGTIREMRLNPQNVQNVVTYNVVIDVSNPEQR
                     LKPGMTANLEITIDERNNVLKVPNAALRFTPQGETVQRRRAGQGEGAGSSSAQGSRRD
                     SQGAGNANGASVENATGAGGAQAEGARERVAPATSPVLEGQTRRVWVLGQDGKPQPRR
                     IKVGLTDGAATEVLEGDLREGEIVVTGQNITGANRPQTGQTAAPGFGGAPRTGGAGGA
                     GGGRRR"
     gene            complement(7479..8246)
                     /locus_tag="VGB76_04135"
     CDS             complement(7479..8246)
                     /locus_tag="VGB76_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098126.1"
                     /translation="MRNFYCSRLVFALFALSIWGGTASAQTLTPAPEEELARTRAELI
                     GATQEYKASTENLLRLEEEEVNRSAERVEELRQLFAEGVIARVELEESERALVAARER
                     LEERRQQIAVSDSLISEIKAAEELAKAEPPPSQPVQTPSVPKGSYSSSGVVIRYTGKS
                     YWSIAGLETVQAFFSTRFGRTLPVSALGQTATHNRLGFDHRHAVDIALHPDSVEGRAL
                     IDYLQSRGITFIAFRAAVPGSATAPHIHIGAGSRRLT"
     gene            8457..8810
                     /locus_tag="VGB76_04140"
     CDS             8457..8810
                     /locus_tag="VGB76_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098127.1"
                     /translation="MKKVFVAIMCLVLLTASAMPTLAQTSHNRNARYTAQQRRADNSR
                     RANYERRDTDGSFIEEHRDKLTVAAGTGVGAAVGAAAGGKKGAIIGALLGAGGSALYT
                     YVLRDKDDDGRYYRR"
     gene            8858..9562
                     /locus_tag="VGB76_04145"
     CDS             8858..9562
                     /locus_tag="VGB76_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0098128.1"
                     /translation="MKQVEPAGGERVRFPARHLCTGARLPNDYEQAQDLELARLAREG
                     DEAAFAEIMRRYSPRVFRFASRYFRQRSLVEEAAQEVFLKAFTQLDSFEGRGSMEGWL
                     TRITTNTCLNLLRSAKRRPELTVSNLSEDESTWLDDMLAGTAWERHRAGERSLVAADL
                     AGRVLETMSPEDQLVLMLVDGEDTPVKEVAEMTGWSVSKVKVQAFRARRKMRKAVEQL
                     LSHKRKNPDESLETAR"
     gene            9566..10060
                     /locus_tag="VGB76_04150"
     CDS             9566..10060
                     /locus_tag="VGB76_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098129.1"
                     /translation="MSDEHIIGMIENAPLSGLSESELAHIRAHTALCAGCGRAFEAAQ
                     LSTLLLRERAAETVEPSPFFQTRVLAALRERQAVRETSALRRLWNASGALVSSMAATV
                     AALVVLTVFAPGSQPASAPGEVASAANSYSAEEVLFEQGDANAEPMSYGQVLSTLYES
                     DDDR"
     gene            10068..>10461
                     /locus_tag="VGB76_04155"
     CDS             10068..>10461
                     /locus_tag="VGB76_04155"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEY0098130.1"
                     /translation="MDMNSKNRWQVRAAALLIFLLGFTAGALALNAYRAWSRGDAGER
                     RQDRFEQMSERLKLNDEQKSQVQKILGDTREQLQGLRKEAEPRVTEIRRQADEQLQKT
                     LTPEQWQQFQQMRDEMRGRGGRRRRGSDR"
BASE COUNT         1913 a         3255 c         3296 g         1997 t
ORIGIN      
        1 tgcaggcgtt cgagatagag gatgcggctg ccggtcagta gaaagaggaa cgcgcccgcg
       61 tagagagtcg cttcgcagac gaacagcagg cgcgcgacgg aaaggcgacg ccgcctgtgt
      121 agggagcggc gtcgccttgc acggagtcgc gggcgggcaa tgcgcggaaa gactctcatc
      181 cccggcctta ctcgtatctg agcgcgacca tcggatcgac gcgcatcgcc cgccgcgcgg
      241 gaatgtagca ggccagaagc gccacgaatg tcaacagcag cgacacgcct aggaaggtca
      301 gcgggtcggt ggcctgcact ccgaacagca gcgtctccat caaacgcgtc agcgcgagtg
      361 cgcccgccac gccgatcacg atgccgatga ccgtgagcat caatccctga ccgagcacga
      421 gtttcaagac atccttcggc tgcgcgccga gcgccatgcg gatgccgatc tcgcgcgtgt
      481 gttgcgtgac gaggtaagac atcacgctgt agatgccgat ggtggaaagc agcatcgcga
      541 gcgcggcgaa gatgctgagc agcatcatgt tcaggcgctg ccttgagatg gattcgtcga
      601 gcacctgctc catggtcagc acgttgtaga cgggctgcgc cggatcgacc gcgagcacct
      661 cgcgccggat cgcctgcgtc aaggtcgtcg ggtcggacga agcgcgcacg acgagggaca
      721 tgttctgcgt catccccgcc cagaaaggcg agttgtaggg cagaaacatt tcggggcgcg
      781 gcggcgtggt caaccctgtc tgtttcacgt cgcgcaccac gccgacgatc tgaatcgggg
      841 tgccgtcgct ttgaatctgc ttggcgagcg cgtcgcgccc gttcaggatc acgcgcacga
      901 aggtttcgtt gacgatggcg acgggagctt gctcgcgcgc ggcaggctcg aagaagcgtc
      961 cggccacgag cgggatgccg agcgcgcgga agtagtcggg cgtggcggtg cgatactcta
     1021 cgagcgcgcc ctgtcccgaa ggggtgggcg ggtcgccgac gatctggacg ttgccgttcg
     1081 tgccggagcg ttggacgggc aggaggttga tggctccggc ggcctcgacg ccgggcagcg
     1141 cggagacgcg ttcgagcacc tgcgtataaa aattggtggc cgcgtcggtc gtctgatact
     1201 tcgccgccgg tagcgtgacg cgcatcgtca aaacgttttc gggtctgacg cccgactcca
     1261 cctgctgcaa gcgtatgaag ctcttgacga gcaagcccgc gccgaccagc aacacgaccg
     1321 ccgaagcgac ctgagccacg gcgaggatgc tgcgcaacca gttggcgcgc gggttggaac
     1381 cggaagtgcc cccgtctttg agcgcgtcct gcacgtcggc cttggaagtt tgcagcgcgg
     1441 gggcgaggcc gaagatgatg cccgtcagga gagagagcag gagggtgaag ccgagcactc
     1501 tgaggtctaa ccccacctcg ctcgcgcgcg gcagaacgcc cgtggtcaaa ctcagcaacg
     1561 cgtcaatgcc ccacttcgcc accaccagac cgagcagccc gccgaggatg gacaacaaca
     1621 cgctctcggt cagaaactgc cgcaccagcc gtgcgcgacc cgcgccgagc gcggtgcgga
     1681 tagcgatctc cctgcgcctc gcggcggcgc gtgccagcag caggttggcg acgttcgtgc
     1741 aggcaatgag caggacgaag cccaccgccc ccagcaggat caacaaggcc gggcgcacgc
     1801 tcttgacgat ctcctcctgc aagcgaccga cggtgatgcc gcgtttggct tgctggtctg
     1861 gatactgttg ttcgagtcgg cgcgcgatga cgttcatctg ctcctgcgcc tgttccaaag
     1921 tcacgcccgg tttcatccgc ccgatggacg agaaggcatg gttgccgcga ttgcccgcct
     1981 gcctgtccgt gaagacgaga ggcacccaca actccgtggt ccgggaggga aactggaagc
     2041 ccggcggcat cacgccgacg accatataat tttcgccgcc gatcagcacc tcgcggttga
     2101 ggatgcgcgg gtctgcgccg aagttcctct gccagagttg gtcgctcagg atgacgacgc
     2161 ggttcctgcc cgccgtgtct tcgcccgtaa tgaaggtgcg cccctgcttc ggcgcgacgc
     2221 cgagcacgtc gaagaagttg gccgtcacgg acgcgcccgc ggagcgttcg ggcaggtcgc
     2281 ttcccagcag gctgaagttg ccctgctcgt aagcggcgat ctgctcgaag agatcattct
     2341 gctcctgcca gtctctaagg ttggggacgg agacggagcc ttcgccgcct tgcggcagac
     2401 gttcccagag atagacgatc tgatccgggt ctttgtaggg cagcggcctg agcagcaccg
     2461 cattgacgac gctgaagatg gcactgttgg ctccgatgcc gagagctaag gtcagcacgg
     2521 cgacgaaggt gaaaccgcga tgcttgaaca gcatccgcac gccgtaacgc aagtcctgta
     2581 aaaacgtctc gaagattccc actccgcgca cctcccgaca ttcttcttta attcgttcaa
     2641 agccgccgaa acttctctgg gcggcatcgc gagcctcttt cagactcatc ccccggcgca
     2701 ggttctcttc gatctccctt tccagatgaa agcgcagttc cctgtccaac tcacgctcca
     2761 tctgctcgcg ccggaagagc gcacgcagcc atagtttcga cttctcccac atcgccacag
     2821 ctccgctcag gtggttttaa ggataaggct gatcgcgccg gagaagcgct cccacatcgc
     2881 ggtttcggcg gcgagttgcc gtcgtccggc ggcggttaac ttgtagtact tggcctgccg
     2941 gttattgtcg gacgtgcccc actcggagcc gatccacccc tgcgcttcga gacgatgcag
     3001 agccgggtag agagagcctt gattgacgcg cagcacgtcc tgagaaatct gttgaatgcg
     3061 ctgcgagatg tcccacccgt gggtcgcacc gagcgcgaga attttaagga tcagcagatc
     3121 gagcgtgccc tgcaacaggt ctgttttttg tttcgccata agactcctgt agataaacga
     3181 cagaggcgca gattatgttc cttcttgtag attgtcaagg ggaaggccgg ttttccattt
     3241 tctcggatca acgatgcgga aaagttggat cgtgaagatg cgctaaaccg ggtcggctgg
     3301 cgggaatcgc tctaaggacg ggcgaaggct cgcggaacac ttccgtccag tcggaaatgc
     3361 cccgcgagcc ttcgcccgtc ctgcgtcaca atttcgacgg gatgatttac tcgtaacgta
     3421 aagcgtctat cgggtcgagt gcggcggcct tacgcgccgg gtagtagccg aaggagatgc
     3481 cgacgaccgc cgagaagatg accgagccga cgatggccgt ggtggagacg agcgtaggcc
     3541 agccgagcag catcgggatg gtgacggaga cggcgatgcc gagcaggatt ccgatcagcc
     3601 cgccggttaa gctgagcacg attgactcga tcaggaattg cctgcggacg gcggacgagc
     3661 gcgcccctat cgccatgcgg atgccgatct cgcgcgtgcg ttcggtgacg gagacgagca
     3721 tgatattcat gatgccgatg ccgcccacga ggagcgagac gcccgcgatg ctgccgagga
     3781 gcatcgtcat aatattgttg gtttcgttgg ccgcctccgc cacgtccgtc atgttgcgca
     3841 cggtgaagtc gtcgggcgcg cccgccgtga gtttatgacg ctggcgcagg agttccgcga
     3901 tctgcgtctg cgccgtgtag gtggcatcct gcgagatggc ggagacgtat gcgacttgca
     3961 gacgcgtgtt ccccagaagc tttttctgca cggcggtgta gggggcaaaa gccgtatcgt
     4021 cctgatcgcg tccctgcggg tcttgaccct tgcgcgccat cacgccgacg accttgaaag
     4081 gaagattcat cacgcgcacg gattgcccga gcgggtctgc gccgggaaac aggttatcgg
     4141 cgatggtctg gccgagcacg atgacacgtg cggaggcacg cacgtctgcg tctgtgaaga
     4201 acgcgccgct cccaaccgtc cacttgcgca cttcgggaaa ctgttcgctc acgccctgaa
     4261 cagaagtgtt ccagttctgg ttgccgacga cgatctgcgc ccgcgcgttg acagacggcg
     4321 tgaccatcga aacgctcggc acttcgcgcc gaatggcctc aaggtctgcg acggtgagcg
     4381 tgttcgtgcc gctgtcgccc gcaccgctgc gcacgccgcc cacgttctgc gcgcccgcgc
     4441 tgacgaagag cagattcgtg cccatgcttt cgatctgagc ctgcacggag gcttgcgctc
     4501 cctgcccgat gctgaccatc gcaatcacgg ctgagacgcc gatgatgatg ccgagcatgg
     4561 tcagtgcggc gcgcatcttg ttgcgcacga gggcgcgaaa agcgacgcga ataatcatta
     4621 acaagttcat ggtctgctaa cccttcaggc ggtcgaatgt cgccgccgtc tttcaatctt
     4681 cttcctgatc cacatcgggc agaagtctca actcctccga ggcatcgcgc cggttttcga
     4741 cagggtagtc ttccttgatc ctgccgtctt tgaagacgac gacgcgatcc gcatactcgg
     4801 cgatgtcagg ctcatgcgta acgaggacga gcgtgatgcc gcgttcgcgg ttgagcttct
     4861 gaaaaatctc catcacttca atcgaagtgc gcgagtcgag gttgccggtc ggctcgtctg
     4921 cgagaatgag cgcgggtcgg ttgacgagag agcgcgcaat ggcgacgcgt tgttgctggc
     4981 cgccggacag ttggttcgag tggttgttct cacgccccgc caagcccacc gcgcgtagcg
     5041 cctcccgcga acgactgtga cgctctgccg tctcgatgcc cgcgtagagc atgggcagtt
     5101 cgacgttctc taatgccgag gtgcgcgaga gcaggttgaa gccctgaaag acgaagccca
     5161 gtttgcggct gcgtatgtcg gcgcgctcgt ccttcgacat cgtggacacg tcttcgccgt
     5221 cgaggatgta actgccctgc gtcgggcggt cgagacagcc gatgatgttc atcgtcgtgg
     5281 atttgcccga acccgacgtg cccatgatcg cgacgaactc gccttttctg atcgtcagag
     5341 agattcctcg cagggcatgc acctcgacat cgcccatcgt gtaagtctta tgaatcccct
     5401 ccaactcgat cacgggcggc gcggccttcg cgcgcgcgag cagccgtgcg tcgagcttaa
     5461 gttcctgcgg tttgatctca agtgttgcct gcattgcttt ctccctatcc tgctgtgaac
     5521 ctttgacagc cggggatacg tgagggcgtt tccacggctg tcacgagttg agcctcgcgg
     5581 cttacctgcg tctgccgccg cctgcaccgc ctgcgccgcc cgtgcgcggc gcgccgccaa
     5641 agccgggtgc tgcggtctga cctgtctgcg gcctgttcgc gcccgtgatg ttctggcctg
     5701 tgacgacgat ctcgccttcg cgcaggtcgc cttcgagaac ctcggttgcg gctccgtccg
     5761 tcaggccgac cttgatccgg cggggctgcg gcttgccgtc ctgaccgagc acccagacgc
     5821 gacgggtctg gccttccaag acgggcgaag tggccggggc gacacgttca cgcgcgcctt
     5881 ccgcctgcgc cccgcccgcg cccgtcgcat tctccacgct cgcgccgttc gcatttcccg
     5941 ctccctgaga gtcgcgccgg gaaccttgcg cggaactact gcccgcgcct tcgccctgcc
     6001 ccgcgcgccg acgctgcacg gtttctccct gtggcgtaaa acgcagagcg gcgttgggga
     6061 ctttgagcac gttgttgcgc tcgtcaatcg tgatctcaag attggcggtc atgcccggct
     6121 tgagtctctg ctccgggttg ctaacgtcga tcacgacgtt ataagtgacg acgttctgga
     6181 cgttctgcgg gttgagccgc atctcccgga tcgtgcccgt gaactccata cccggaaagg
     6241 catcaaccgt gaagctcacg ctcttcgcct gttcgaccaa accgatgtcc gcctgatcta
     6301 tgttcgccat gacctgcatc tgcgtcaggt cgttggcaat cgtgaagagc gtcggggcgg
     6361 agagactcgc ggcgaccgtc tggccgatgt ccacgtcgcg cgagacgacc acgccgtcaa
     6421 tgggcgagcg gatagtcgtc tgcgagagat taatttcggc cagttggagc gcggcctgtg
     6481 cctgctgcac tccggcggcg gcgttctgca cctgagccgc cgcgttctgc gcctgcgcct
     6541 gtgcctgctg cactcccgcc tgtgcctgct ggattcccgc ctgcgactgc acgacccccg
     6601 aactcgaagc cgactgcgcg ctcaactgcg cctgttgcac ttgcgcggcg gcctgctggt
     6661 agcgtgcttc cgccgtcttg taactcgtct gcgccgtctc cacctcccga cgtgcgatga
     6721 cgccgctcga cgcgagcgac tcctgttgcc gtaagaaact ggccgcgtcg tccatctgcg
     6781 ctttcagcac cgcgaggttg gcctgcgcgc cgtagacgcc cgcctgctgg ttctgcaccg
     6841 tcgatctggc ggccaacgct ttggcctgcg cgtccgtaac gactgcacgc gattgactga
     6901 cgcctgcgcg cgactgcgcg acgcccgcct gcgcctgctg gagcgcggcg cgtgcctgca
     6961 cgaggttggc gcgtgcctgt tcgacctgcg cctgtgcggt cgccgggtcg agttgcgcga
     7021 taacctgccc cttcctgacc atcgagttga aatcggcgtg gagcgcggag atcgtgcccg
     7081 acgcctgact gccgacctgc accgtcgtga cagcttgaag cgtgcccgtc gccgtgaccg
     7141 agttacgcaa gtttcccctc tccactttcc ccgtcatgta ctcggctgcg ctcgcacggt
     7201 cgccccacaa aacgaacccc gcaaacgcga cgagcgcgac cacgcccaac gctattacga
     7261 aacgctttct gccgctcaaa tttgatttga tccacttcat gattctcttc ctatcgaggc
     7321 gcacccgccg tccgttcgtt aatgcccgat ttaatcaaag agtctgcgtg caaactctgc
     7381 gcacactctg atagacaccg gcgacgcccg ttgcgttaca cccggcgtgc ctcgaaatcg
     7441 cgcttcgatt agttcgcttc gtgtgggctt cttttaaatc aggtgagacg gcgcgagcct
     7501 gcgccgatgt gtatgtgcgg ggctgtcgca gagccgggca cggcggcgcg aaaggctata
     7561 aacgtgatgc cccggctttg caggtagtcg atcagcgcac gcccctccac gctgtcgggg
     7621 tgcaacgcga tgtcaaccgc gtgtcgatga tcgaagccga ggcggttatg cgtcgccgtc
     7681 tggccgagcg cgctgaccgg gagcgtgcgc ccgaagcggg ttgaaaagaa ggcttgcacc
     7741 gtctccagac ccgcaatcga ccagtagctc ttccccgtgt agcggatgac gacgccgctc
     7801 gaactatagc tgcccttcgg cacggacgga gtttgtaccg gctgcgacgg cggcggctct
     7861 gcttttgcca attcttcagc cgctttgatc tccgagatca aactgtctga gacggcgatc
     7921 tgctgacgcc tctcttccaa cctctcgcgc gcggccacga gtgcgcgctc gctctcttca
     7981 agctccaccc ttgcgatgac cccttccgcg aaaagttgtc gcagttcttc gaccctctcc
     8041 gcgctcctgt tgacctcttc ctcttcaagc cgcaacagat tttcggtgct ggccttgtat
     8101 tcctgcgtcg cgccgataag ttctgcgcgc gtgcgtgcga gttcctcttc cggcgcgggc
     8161 gtcaacgtct gcgcgctcgc cgtgccgccc cagattgaga gcgcgaacaa cgcaaaaact
     8221 aaacgcgaac aatagaagtt tctcatgccg gtttctccgt cttccctctt actccagaca
     8281 cctccctgac cccggaagtt acagagagac gcggagcggc aactttctcc cgcgcaattg
     8341 aacggagcgt tgtaaccctt cgacgaagcc cggtgtctta cctcgcagaa ggagattgaa
     8401 gagccttcgc aggaaacagt ttggctctac cgaaacagga aggaagaggt aaaggaatga
     8461 aaaaggtatt tgtagcaatc atgtgtctgg tactgctcac ggcgtcggca atgcccacgc
     8521 tggcgcaaac gtctcataac cgcaacgcgc gctacaccgc gcagcaacgg cgcgctgaca
     8581 actcgcgccg cgccaactat gaacggcgcg atacggacgg ctcttttatc gaagagcatc
     8641 gtgataaatt gaccgtcgcg gcgggcacag gcgtcggcgc ggctgtcggg gcggcagcgg
     8701 gcggcaagaa gggcgcgatc atcggcgcgc ttctcggagc gggcggctcc gcgctctaca
     8761 cttacgttct gcgcgacaag gatgacgacg ggcgttacta tcgtcgctga gagcgtcggt
     8821 tggtgtgaca gagtgtgacc gccacacttt gtcacaggtg aagcaagtgg aaccggcggg
     8881 cggggagcgt gttcgattcc ctgcccgtca tctctgcaca ggggcgcgtc tgccgaacga
     8941 ctacgaacag gctcaggact tggagttggc acgtctggca cgtgagggcg acgaagccgc
     9001 cttcgcggag atcatgcggc gttacagccc gcgtgtcttc agatttgcca gccgctactt
     9061 tcgccagcgc agcctcgtcg aagaggccgc gcaggaggtc ttcctcaagg ctttcacaca
     9121 gctcgacagc ttcgaggggc gcggctcgat ggagggctgg ctgacgcgca ttacgaccaa
     9181 cacctgtctc aacctgctcc gcagcgcaaa gcgaaggccg gagttgaccg tctcgaacct
     9241 gtcggaagat gagagcacat ggttggacga catgctggcg ggcacggcct gggagcgtca
     9301 ccgggcggga gaacgaagtc tcgtcgctgc cgacctggca ggccgcgtcc tcgaaacgat
     9361 gtctcccgaa gaccagttgg tcttgatgct cgttgacggc gaagacacgc ccgtcaaaga
     9421 ggtggcggag atgaccggct ggtctgtctc gaaggtgaag gtgcaggcat ttcgcgcgcg
     9481 ccgcaagatg cgcaaggcgg tggaacaatt gttgtctcat aagagaaaga acccggacga
     9541 gagtttagaa acagcgaggt gagagatgag cgacgaacac atcattggca tgatcgagaa
     9601 tgcgccgttg tccggtttga gcgagagcga gttggcgcac atacgcgcgc atacagccct
     9661 gtgcgccggg tgcgggcgcg cttttgaagc ggcgcaactc tcgacgcttc tactcagaga
     9721 acgcgcggcg gagacagtgg agccgtcgcc tttcttccag acgcgagttc tggccgcgct
     9781 gcgtgagcgg caggcagttc gagagacttc cgcgctccgg cgtctatgga acgcgtcggg
     9841 cgcgctcgtc tcctcgatgg ccgcgacggt cgccgcgctc gtggtcttaa ccgtcttcgc
     9901 gcccggttct cagcccgcat ccgctccggg tgaagtggcc tctgcggcca actcctattc
     9961 ggcggaagag gtgctcttcg agcagggcga cgcgaacgca gagccgatgt cttacgggca
    10021 ggtgctctcg accctctacg aatcggatga tgacaggtga aataattatg gacatgaaca
    10081 gtaagaacag gtggcaggtt agagcggcgg cgttactgat attcctgctc ggcttcacgg
    10141 cgggcgcgct cgccctgaac gcctatcgcg cctggtcgag gggcgacgca ggagagcgca
    10201 ggcaggatcg cttcgagcag atgtccgaac gcctgaagct caacgacgaa cagaaatctc
    10261 aagtgcagaa gatactgggc gacacgcgcg aacagttaca gggtttacgc aaagaagccg
    10321 agccgcgcgt gacggagatt cgacggcagg ccgacgagca attgcagaag actctgacgc
    10381 cggagcaatg gcaacagttt cagcagatga gagacgagat gcgcgggcgc ggaggacgac
    10441 gcagaagagg gtcggacaga g
//
LOCUS       DASWNS010000123         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_56808, whole genome shotgun sequence.
ACCESSION   DASWNS010000123 DASWNS010000000
VERSION     DASWNS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56808"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="VGB76_04160"
     CDS             <1..1887
                     /locus_tag="VGB76_04160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0098131.1"
                     /translation="SPDSRRLAFFATRGEQSGLWVMSPRERQSPRFVVAVRSTNYHIP
                     YAGESFAWSPDSRRLAYINATDEAQDNAPATSATGDARARRDDPRLVDRIQYKSRTSF
                     SDTLRTHVWLTDVDEPAPRQLTSGLHYDHALTWSPRGDEIAFLSNHEPDPEAVNNSDI
                     FAVALDGRTRQITSTRGCEYEPAWSPDGRWLAYTATTRDVTTIDSVAEDTHIWVIEAG
                     GGGKAREQAAALDRRARSPRWLPDSNALLFLAGDNGRTTIYGFRLNDRQVHRLSLWTD
                     GVGFRMLFDAQSRTAPTTTDALPIRPARPFQISNFSIASLSSRLPAAATSLNLTPLAF
                     TLGNAVAPAEVWFGLGASSVPLRRLSNHNEAFLRSVSVVEPEEIRFKSFDGTDVQGWL
                     MKPLNFRDNERYPLILSIHGGPHGMYGYSFNAAFQTYAARGYGVLFINPRGSSGYGQK
                     FSDGTLREWGGGDYRDLMTGVDEALRRFNWIDRGRMGVTGGSYGGFMTNWIITQTPRF
                     RAAVASASVSNLVSFYATSLYQDLIHAEFGGFPWDDYDLLWRWSPLRYVRAVETPTLF
                     IHGELDNDVHITQAEEMYMALRRRGVETVLARYPREGHGFREPRHREDALERTVAWFD
                     KYLK"
     gene            1986..2282
                     /locus_tag="VGB76_04165"
     CDS             1986..2282
                     /locus_tag="VGB76_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEY0098132.1"
                     /translation="MLTHIVIWKYLAETTADEREEHVARLRRLPNFIPQIESFAVGFD
                     VLHLPRSYDVGLVAVFRDRAGLDAYTDHPEHVLVAVYGRSLSEQIASVDFESAD"
     gene            2391..3611
                     /locus_tag="VGB76_04170"
     CDS             2391..3611
                     /locus_tag="VGB76_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098133.1"
                     /translation="MRRLLKLLAFVLVVALLSQTPFIYRRYQLGRLEAKIRELNAARE
                     VSPDPVYADYKGVVHVHSFLGGHSTGTFAEIERAAQANSLAFVVMTEHPSNDYDTRAL
                     TLRGVRGGVLFIQGSETSESAEDRLLTVGGTTTAGETTADTTTQASVNRAKAANQLVF
                     IAHPETFRSWRETGGFDGMEIYNLHADAKNINRLALFFDGLWSYRSYPHLLWTRFYQS
                     PDANLRRFDELTAPEGGRKLVAIAGNDAHANIGLSLRQLTGKPFLELKLDPYERSFQV
                     VRTHVLLEHAKPLNEENLLDALARGHAYVSFDILCDASGFRFTASNGAEQKLSGDEIE
                     LASGVRLEVKTPVRSRIVIFRDGRAVSEAKEAARHEWTAKEAGVYRVVCYLPQLPAPL
                     DAKPWIISNPVYVR"
BASE COUNT          722 a         1235 c         1157 g          707 t
ORIGIN      
        1 tcgccggaca gcaggcggct cgccttcttc gccacgcgcg gcgaacagag cggcctctgg
       61 gtgatgtcgc cgcgcgaacg ccagtcgccg cgtttcgtcg tcgccgtgcg ctcgaccaac
      121 tatcacatcc cttatgcggg cgagtcgttc gcgtggtcgc cagacagtcg ccgcctcgcc
      181 tacatcaacg cgaccgacga agcgcaggac aacgcccccg cgacgagcgc gacgggcgac
      241 gcacgcgcgc gacgcgacga cccgcgcctc gtggatcgca tccagtacaa atcgcgcacc
      301 tctttttccg acacgctgcg cacgcacgtc tggctcacag acgtggacga acctgcgccg
      361 cgccaactca cttccggcct acactacgat cacgcgctca catggagtcc gcgcggcgac
      421 gagatcgctt ttctctccaa ccacgaaccc gacccggagg ccgtcaacaa ctcggacatc
      481 ttcgccgtcg cgctcgacgg tcgcacgcgc cagatcacct cgacgcgcgg ctgcgaatac
      541 gaacccgcat ggtcgcccga cggcaggtgg ctcgcctaca ccgccaccac gcgcgacgta
      601 acgaccattg acagcgtcgc cgaagacacg cacatctggg tgattgaagc ggggggcggc
      661 gggaaggcgc gcgaacaggc cgccgcgctg gatcgtcgtg cgcggagtcc gcgctggttg
      721 ccggacagca acgcgctcct ctttctcgca ggcgacaatg ggcggacgac gatttacggt
      781 ttcagactca acgacaggca ggtgcacagg ctctcgctct ggactgacgg cgtgggcttc
      841 cgcatgctct tcgacgcgca gagccgcacc gcgccaacca cgacagacgc tctgccgatt
      901 cgtccggcgc gtccgtttca gatcagcaat ttcagcatcg cctctctctc gtcgcgcctt
      961 cctgccgccg cgacgagcct caacctcacg ccgctcgcct tcacgctcgg caatgcggtc
     1021 gctccggcgg aggtctggtt cgggcttggc gcgtcgtccg tcccgctgcg ccgcctgagc
     1081 aatcataacg aggcgtttct acgctccgtc tcagttgtcg aacccgaaga gatacgcttt
     1141 aaaagtttcg acgggacgga cgtgcagggc tggctgatga agcctctgaa cttccgcgac
     1201 aacgaacgct acccgctcat cctgagcatt cacggcgggc cgcacggtat gtacggctac
     1261 agtttcaacg cggcctttca aacttacgcg gcgcgcggct acggcgtgct cttcatcaac
     1321 ccgcgcggtt cgagcggcta cgggcagaag ttttccgacg gcacgctgcg cgaatggggc
     1381 ggcggcgact accgcgatct catgaccgga gtggacgaag ctctgcgccg cttcaactgg
     1441 atagatcgcg ggcgtatggg cgtgacgggc ggctcttacg gcggtttcat gaccaactgg
     1501 attatcacgc agacgccgcg ttttcgtgcg gcggtggcga gcgcgtccgt gtcgaacctc
     1561 gtcagttttt atgcgacctc tctgtatcag gatttgatcc acgcggagtt tggcggcttc
     1621 ccgtgggacg attatgatct cctgtggcgt tggtcgccgc tgcgctacgt gcgcgccgtc
     1681 gagacgccga cgctcttcat tcacggcgaa ctcgacaacg acgtgcacat cacacaggcc
     1741 gaagagatgt acatggcctt gcggcggcgc ggcgtcgaaa ccgtgctcgc gcgctatcca
     1801 cgcgaaggcc acggcttccg cgaaccgcgc catcgcgaag acgcgctcga acgcaccgtc
     1861 gcgtggtttg ataaatatct gaaatgagaa tgcagaattc aggagccgga attcggaaga
     1921 aagcgttatg ctgcctcttt gattctgaat cctgaattct gactcctgaa tcctgactcc
     1981 tgcttatgct gacacacatt gttatctgga aatatctggc ggagacgacc gcagacgagc
     2041 gcgaggaaca cgtggcgcgt ttgcggcgtc tgccgaattt catcccgcag atcgagagct
     2101 tcgccgtcgg cttcgacgtg ctgcacctgc cgcgctcgta tgacgtggga ctcgtcgcag
     2161 tctttcgcga ccgcgcgggg ctggacgctt acacggatca tccagagcac gtgctcgtcg
     2221 cagtctatgg acgcagcctc tcggagcaga tcgcgtccgt cgatttcgag agcgcggatt
     2281 gaagcgagac gaaagatgag agcgttgcta tccgagatgc accggggcgg agcggagtcg
     2341 cctcgcgcgg cgcgcgtgcc gtcgtttaga cgcccgcgcg actgaacccg atgagacgtt
     2401 tgttgaaact actggcgttc gtcctcgtcg tcgcgctctt gtcgcagacg cccttcatct
     2461 atcgacgcta ccaactcggt cgcctcgaag cgaagattcg agagttaaac gcggcgcgcg
     2521 aagtttcgcc cgatccggtc tacgccgact acaaaggcgt ggtccacgta cactcttttc
     2581 tcggcgggca cagcacgggc acgttcgcgg agatcgagcg ggcggcgcag gccaacagcc
     2641 tcgccttcgt cgtgatgacg gagcacccgt cgaatgatta cgacacgcgc gcgctgacct
     2701 tgagaggcgt gcgcggcggc gtgctcttca ttcagggcag cgagacgagc gagtctgctg
     2761 aagaccgcct gctcaccgtc ggcggcacga cgacggcggg cgagacgaca gccgacacga
     2821 caactcaagc gagcgtcaat cgcgcgaagg ccgcaaacca acttgtcttc atcgcgcacc
     2881 cggagacgtt tcgcagttgg cgcgagacgg gcggcttcga cggcatggag atttacaacc
     2941 ttcacgccga cgccaaaaac atcaaccgcc tcgcgctctt cttcgacggc ctctggtctt
     3001 accgcagcta cccgcacctg ctctggacgc gtttctacca aagccccgac gcaaacctgc
     3061 gacgcttcga cgaactgacc gccccggaag gaggccgtaa gcttgtcgcc attgcgggca
     3121 acgatgccca cgcgaacatc ggcctcagcc tgcgacaatt gaccggcaag ccgtttctcg
     3181 aattaaaact cgatccatac gagcgcagct ttcaagtcgt ccgcacgcac gtgctgctcg
     3241 aacacgcgaa gccgctcaac gaagagaact tactggacgc gctcgcacgc ggccacgctt
     3301 acgtctcttt cgacatcctg tgcgatgcga gcggctttcg cttcacggcg tcgaacggcg
     3361 cggagcagaa gttgtcgggc gatgagatcg agttggcttc gggcgtgcgt ctggaagtga
     3421 agactcctgt gcggagtcgc atcgtgatct ttagagacgg tcgggcggtg agtgaagcga
     3481 aggaggccgc gcggcacgag tggacggcga aggaggcggg cgtctatcgc gtcgtctgct
     3541 acctgccgca actgcccgcg ccgctcgatg cgaagccctg gatcatctcg aacccggtct
     3601 acgtgcgctg agaattcgag cgtccggaca cgacatcgcc ccgacagcta aaactgtgcg
     3661 gggcggtttc gcgagccgga aagctccagc tcaatttttg gaagcggcgt cttaagtcgc
     3721 cttaacttct gtagagagtc acgtgtttga cccttcgcac gcggcacatc tcaagtgtca
     3781 gagaagcgcg tgtacaggct tcctattaag ttgtaccaag t
//
LOCUS       DASWNS010000124         7193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_420863, whole genome shotgun sequence.
ACCESSION   DASWNS010000124 DASWNS010000000
VERSION     DASWNS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420863"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1698
                     /locus_tag="VGB76_04175"
     CDS             55..1698
                     /locus_tag="VGB76_04175"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEY0098134.1"
                     /translation="MAASVLTKESGSPHEEHGRRLSDVGLESLSITQASNVYWNLSTP
                     ELYEEIARRGEGNFSDHGALIVDTGEHTGRAAKDKAIVRDAASADKVFWGEVNKEFST
                     ERFNALRDRMMAHTAGRDLFVQDTFAGADPRYRLPIRVVTELAWHSLFARTMFINPEA
                     GSTEKHTPEFTIVNIPSFKADPARDGTRAETFILMDFTQRLVLIGGTSYAGETKKSVF
                     TILNYLLPQRGVMSMHCSANVGDAGDVAIFFGLSGTGKTTLSADPERRLIGDDEHGWS
                     DDGVFNFEGGCYAKVIRLSSEAEPDIYRTTRMFGTVLENVVYDPVTRALDLDDASKTE
                     NTRAAYPLTSIPNIVPEGHAGHPQNIIMLTADAFGVLPPVARLTPEQAMYHFLSGYTA
                     KVAGTERGVTEPEATFSTCFGAPFMVLHPGTYADLLGKKLTEHKANCWLVNTGWSGGP
                     YGEGQRMKIKYTRAMIRAILNGSLAEVETQSDPVFGVGVPVSCPEVPAEVLTPRNTWK
                     DTEAYDRKARELAERFNRNFKKYEDGVSEAVRAVAPKAG"
     gene            complement(2020..2952)
                     /locus_tag="VGB76_04180"
     CDS             complement(2020..2952)
                     /locus_tag="VGB76_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098135.1"
                     /translation="MKVWRVFIANLLVVLLLTTGSRVVVAQAASGAPEANDVAKITAE
                     EEREAREVAERFMRRMQETNDLTPLVGEMFVPDYAARLRQEASGARLPMLSKEAVAQA
                     SREELARYQQALNHSFYMGILLHLAYQNAHPEKAVDEEARGLEYFKQVLPPDIVELCM
                     NDPTLRVLLEEETNEGAEADQPGAPVENADEEDDEPRPVRSVEQLRSFTSTMEQFIVL
                     ARKHLAASTRKQTFIERHKGANEEENWQAERKAVTASAWTLNREFYGYPKGTRIFCVH
                     VLVYHMDLVRVDGRLKVLALYPDMEPPPCAPLAN"
     gene            complement(3054..3659)
                     /locus_tag="VGB76_04185"
     CDS             complement(3054..3659)
                     /locus_tag="VGB76_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0098136.1"
                     /translation="MRRKKLYALIALFLLAPLCAHAQSVLDEHRFEVGGQLTALGVED
                     VSEPLLGVGARVGYNFNKYLALEGEANFFPSDAFGNDQIGQRAQAFAGIKAGARSRYA
                     GLFAKARPGVMFIGETISGFNCRRLDGFTSCRPSHRNFALDAGAVAEFYPTRRSIIRL
                     DVGDTMVNVRRATRNVFNQTSTSSSEFIHQLQIGLGFSYRF"
     gene            complement(3755..4294)
                     /locus_tag="VGB76_04190"
     CDS             complement(3755..4294)
                     /locus_tag="VGB76_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098137.1"
                     /translation="MRHRAQKIENRLRRAVQLSFVLLIACTAMSAQAQTAPNSNQGYS
                     DSDANAASRTRTTNTKGRGAPLGDTPTDILRAARTIHISPNQHINADYLEYKLDKLPE
                     FAQWQLSIVKDSAKADLVLEIRKSALNYIFSIVEPESSIVVAKGKVVAINGRVAAEDI
                     SHQIVKRMRALRALPANER"
     gene            4600..6264
                     /locus_tag="VGB76_04195"
     CDS             4600..6264
                     /locus_tag="VGB76_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0098138.1"
                     /translation="MIDPSDNRRTETNAQGETHAPRGDGGHASSAADAHPGESATTLR
                     SVVAMARRIMRSDTASVATLSVAERMVTWKATSGFNSLGDEDVEIVNPLRGMFAEQAA
                     VSDDMTIIEVRNVVGGELPVSEFPLHSAEGLRDLAIVRLRARGETLGLLVVGYRTAHR
                     LTPDEKELLESLAEMAALALDNARLLETVSAGKKIWEQTFDAMPDGIIVHDASMHLTR
                     CNAMAAEMMGFEQPADAIGLSCANAFARLFGERAAAFHMSPGTTGGIGASSFEIQAED
                     GRRYLVAVAPLDGVAGGDGSSVITWSDVTQISEMQEQLSRSRRLATVGQLAAGVAHEI
                     NNPLAAITTCAEATLRDIRSTPDLSTVAEERSWNFYLEEIVRQALRCKAITRGLLDLS
                     RQRRARREPCDVNALVERSVLLVSQREEAREVSFETALDSQLGEVATDEVLVLQILDN
                     LLTNALDAVEGRGSIRITTMLEGERTRIEVADTGAGIPAEMLVRIFDPFYTTKEAGKG
                     SGLGLAISATLAEALGGALTVESKPGTGSRFRLWLPRRAPETRDEG"
     gene            6324..6692
                     /locus_tag="VGB76_04200"
     CDS             6324..6692
                     /locus_tag="VGB76_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0098139.1"
                     /translation="MKENDLRVRTKVFALRIIKLYTALPGTVEAQVLGKQILRSGTSV
                     GAHYREACRAKSDADFISKIEGGLQELEETIYWLELITESKIFPEERIKPLCEESEEL
                     LAMFVTMVKNVKGKKETLTR"
     gene            6745..>7193
                     /locus_tag="VGB76_04205"
     CDS             6745..>7193
                     /locus_tag="VGB76_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098140.1"
                     /translation="MTRKISVLVVDDDPLLRKLVTEQLSRSGFETAAAASGEEAIEAL
                     QATDYDVALLDIRMTGMSGLHALREIRRLEDPPEVIMLTADTSLGTGIEAMRLGAYDY
                     LTKPSTLDEMEAVIRKADEKRRLVRQNAGLRAAANRGTSAEENAHAL"
BASE COUNT         1432 a         2204 c         2174 g         1383 t
ORIGIN      
        1 actcatcact catcacccat cactcatcac tttttaactt gaggagaatt taagatggca
       61 gcatctgtcc tgacgaaaga atccggctcg ccgcacgagg agcatggccg caggctgagc
      121 gacgtgggat tagaatcgct cagcatcacg caagcctcca acgtctattg gaatctctcg
      181 acgccggaac tctacgaaga gatcgcgcgg cgcggcgaag gcaacttctc cgatcacggc
      241 gcgctcatcg tcgatacggg cgagcacacc ggacgcgccg ccaaagacaa ggccatcgtg
      301 cgcgacgcgg cgagcgcaga taaagtcttc tggggcgagg tcaacaaaga attctccacc
      361 gagcgattca acgccctgcg cgaccggatg atggcgcaca ccgccgggcg tgacctcttc
      421 gtgcaggaca ctttcgccgg agccgatccg cgctatcgcc tccccatccg cgtcgtcacc
      481 gaactcgcgt ggcattcgct ctttgcgcgc accatgttca tcaacccgga agcgggcagc
      541 accgagaagc acacgcccga gttcaccatc gtcaacatcc cgagtttcaa ggccgacccc
      601 gcgcgcgacg gcacgcgtgc ggagacgttc atcctgatgg acttcacgca gcggctcgtg
      661 ctcatcggcg gcacgagcta cgcgggcgaa accaagaagt cggtcttcac gattctcaat
      721 tacctgctgc cgcagcgcgg cgtgatgtcc atgcactgct ccgccaacgt cggcgacgcg
      781 ggcgacgtgg cgatcttctt cggtctgtcc ggcacgggca agaccactct gagcgcagac
      841 cccgaacgcc gtctcatcgg cgacgacgag cacggctggt ctgacgacgg cgtcttcaat
      901 ttcgagggcg gctgctacgc caaagtgatc aggctctcgt ccgaagccga acccgacatc
      961 tatcgcacga cgcgcatgtt cggcacggtg ctcgaaaacg tcgtctatga tcccgtaacg
     1021 cgtgcgctcg acctcgacga cgcgagcaag accgagaaca cgcgcgccgc ttacccgctc
     1081 acgagcattc cgaacatcgt gcccgaaggc cacgcggggc acccgcaaaa catcatcatg
     1141 ctcacggctg atgccttcgg cgtgctcccg cccgtcgcgc gcctcacgcc tgagcaggcg
     1201 atgtaccact tcctctccgg ctacacggcg aaggtggcgg gtacggagcg cggcgtcacc
     1261 gaaccggaag ccactttctc cacctgtttc ggcgcgccct tcatggtctt gcatcccggc
     1321 acttacgcag acctcttagg caaaaaattg accgagcaca aggcgaactg ctggcttgtc
     1381 aacacgggct ggtcaggcgg tccctacggc gaaggccagc gcatgaagat caaatacacg
     1441 cgcgccatga tccgcgccat cctgaacggc tcgctcgcgg aagtcgaaac gcaatccgat
     1501 ccggtcttcg gcgtcggcgt gcccgtctcc tgtccggaag ttccggcgga agtcctgacg
     1561 ccgcgcaaca cctggaaaga cacggaagcc tacgaccgca aagcgcgcga actcgcggag
     1621 cgtttcaacc ggaacttcaa gaagtatgag gacggagttt cggaagccgt gcgcgccgtc
     1681 gcgccgaaag ctggctagag tagacttgaa cgtctgagaa gttgaaggcg actacgcggc
     1741 gcgtagtcgc cttctttttt tgtccggtgt cggtcaggcg aaagttcgcc gtttaaaacc
     1801 tgatgccgaa gttgatgccg ccctgaaaat catgtgtcgt ctcggaaggc gcggtgccga
     1861 caaatacctg ctggacgcct gagccggagt cgggattgag cacgaagggc atcgcgcgcc
     1921 ctcgctcgca ccggacgcgt cgtggctggc ggcttccgat ttctgaattt ttccaatctc
     1981 ttgaatcgcc cgctgccgtg tctgaggcac gggcgcggct cagttggcta agggggcgca
     2041 cggaggtggt tccatgtccg gataaagcgc gagcactttg agtctgccgt caacacgaac
     2101 gaggtccatg tggtagacga gcacgtggac gcagaaaatc ctggtgcctt tcggatagcc
     2161 gtaaaactcc cggttgagcg tccacgcgct cgccgtcaca gccttgcgtt ccgcctgcca
     2221 gttctcttcc tcattcgccc ccttgtgtcg ctctatgaaa gtttgtttgc gtgtggaagc
     2281 ggcgagatgc ttgcgcgcaa ggacgatgaa ctgttccatc gtggaagtga aactgcgcaa
     2341 ttgctcgacg ctgcgcaccg gacgcggctc atcatcttct tcgtccgcgt tttctacggg
     2401 agcgccgggc tgatctgctt ccgcgccttc gttagtctct tcttctaaca acactctgag
     2461 cgtcgggtca ttcatgcata gctcgacgat gtcgggcggg agcacctgct tgaagtactc
     2521 caagcccctc gcttcttcat cgacggcttt ctccggatgc gcgttttgat aagcgaggtg
     2581 taggagaatg cccatgtaga agctgtggtt caaagcctgt tgataacgcg ccagttcttc
     2641 gcggctcgcc tgtgcgacgg cctccttgct caacatgggc aaccgtgcgc cggacgcttc
     2701 ctgtcgcagg cgcgcggcgt agtccggcac aaacatctcg ccgacgagcg gcgtgaggtc
     2761 gttcgtctcc tgcatgcgtc gcatgaagcg ttccgccacc tcgcgcgctt cccgttcctc
     2821 ttccgcagtg atctttgcca catcgttcgc ttccggcgcg ccggaagccg cctgtgcgac
     2881 gacaacgcgg ctgcccgtcg tcagcaacag gacgactaag aggttcgcga taaacactct
     2941 ccaaactttc atgcttttca tctcaatacc ctgaatccct catccctctg accggagagt
     3001 tgcttagaga gggcggcgac aggcgcgccg tcccccgggt ttcgttcgcc gcgttaaaac
     3061 ctgtagctga agccgaggcc gatctgaagt tgatgtataa actcgctcga agaggtcgag
     3121 gtctggttaa agacgttgcg cgtggcgcgc ctgacgttga ccatcgtatc gcccacgtcg
     3181 agacggatga tggagcggcg cgtgggatag aactctgcga ccgcgcccgc gtcgagcgcg
     3241 aagttgcggt ggctcggacg acaactcgtg aagccgtcga ggcgtctgca attgaagccc
     3301 gaaatggttt ctccgataaa catcacgccc ggtcgcgcct tggcgaagag tcccgcgtat
     3361 ctggaacgcg cgcccgcctt gatgcctgcg aaggcttgtg cgcgctggcc gatctggtcg
     3421 ttgccgaagg cgtcggaggg aaagaaattc gcctcgcctt cgagcgcgag gtacttgttg
     3481 aagttgtagc cgacgcgcgc gcccacgccc aggagcggtt cgcttacgtc ttccactccg
     3541 agcgcggtga gttggccgcc gacctcgaac ctgtgctcgt ccaacactga ctgcgcgtgt
     3601 gcgcagagcg gcgcgagcag aaacagagcg attaatgcgt agagtttttt tcgtctcatg
     3661 tgtcctcccc aaaaaaactt tatgagtaat gtgccggcga caagtctgtc gccgttcgac
     3721 aaatgaagtt ggctctccgg cgcgtgcctg tctctcaacg ctcgttcgca ggcagggcgc
     3781 gcagggcgcg catgcgtttg acgatttggt gcgagatgtc ttcggcggcg acgcgcccgt
     3841 tgatggcgac gactttgcct ttcgccacga cgatgctcga ttccggttcg acgatgctga
     3901 aaatatagtt gagcgcgctc ttcctgatct ccagcacgag gtcggccttc gcgctgtcct
     3961 tcacgattga gagttgccac tgagcgaact ccggcagctt gtcgagtttg tattcgagat
     4021 agtctgcatt gatgtgctgg ttggggctga tatgaatggt gcgcgcggcg cgcagaatgt
     4081 cggtcggggt gtcgccgagg ggcgcgcccc tgcccttcgt gttcgtggtg cgcgtgcgcg
     4141 aagcggcgtt cgcatcggag tctgaataac cttgattcga gttgggcgcg gtttgtgcct
     4201 gcgcgctcat tgccgtgcag gcgattaaca ggacgaatga aagctgtacg gcgcggcgca
     4261 ggcggttctc tattttttgt gctctgtgtc tcatctgatc tctccttcgc tgacaggcga
     4321 aactatgctg accgatgacc ttcgcatggc aacgcctgtg ccgcgctacg gcgtgcgata
     4381 aaaggcggga aatcggcgca cgcggctgtg caggctgccg tgcgcgcccg tcacctgacg
     4441 gaaaatcgcg gcggtcgcaa cgagcgcgca ttgcggctcg catttttttc ttgacggacg
     4501 ggcatccacg cagggaagat gacacgcacc cgagccgcga tttagttttt ccacgtccac
     4561 tgaacccgtg tcagcccccg acatccatat ctgaagaaca tgattgatcc ctcggacaac
     4621 cggagaacgg agacaaatgc gcagggggag acgcacgcgc cgcgcggcga cggcgggcac
     4681 gcgagcagcg cggcggacgc gcaccccggc gagagcgcga cgacgctgcg gagcgtggtg
     4741 gcgatggcgc ggcgcatcat gcgctccgac acggcgagcg tggccacgct ctccgtcgcc
     4801 gaacgcatgg tgacgtggaa agcgacgagc ggcttcaact cgctcggcga cgaagacgta
     4861 gagatcgtca atcctctgcg cggcatgttt gcggagcagg ccgccgtctc cgacgacatg
     4921 acgatcatcg aagtgcgcaa cgtcgtgggc ggcgaacttc ccgtcagcga gtttccgctg
     4981 cacagcgcgg agggcttgcg cgatctcgcc atcgtgcgcc tgcgcgcgcg cggcgaaact
     5041 ctgggcttgc tcgtcgtggg ctatcgcacc gctcaccgtc tcacgcccga cgagaaggaa
     5101 ctgctcgaaa gcctcgcgga gatggccgcc ctcgcgctcg ataacgcgcg cctgttggaa
     5161 accgtcagcg cgggcaaaaa aatctgggag cagactttcg acgcgatgcc cgacggcatc
     5221 atcgtccacg atgcctcgat gcacctcacg cgctgcaacg cgatggccgc cgagatgatg
     5281 ggcttcgaac aaccggcgga cgccatcggc ctctcctgcg ccaacgcctt cgcgcgtctc
     5341 ttcggcgagc gcgccgccgc tttccacatg agtccgggca cgacaggggg catcggggct
     5401 tcatctttcg agattcaggc ggaagacggg aggcgttatc tggtggccgt cgctccgctc
     5461 gacggggtgg cgggcggcga tggttcgagc gtcatcacgt ggagcgatgt cacgcagatt
     5521 tccgagatgc aggaacagct ttcgcgctcg cgccgcctcg ctaccgtcgg ccaactcgcc
     5581 gccggagtcg cgcacgagat caacaacccg ctcgccgcca tcacgacctg cgccgaagcg
     5641 acgctgcgcg acatacgctc gacgcccgac ctctccacgg tcgccgaaga acgctcctgg
     5701 aacttttacc tcgaagagat cgtgcgccag gcgttgcgct gcaaagcgat cacgcgcggg
     5761 ctgctcgacc tttcgcgcca gcgtcgcgcc cgacgcgagc cgtgcgacgt gaacgcgctc
     5821 gtcgaacgca gcgtgctgct cgtctcgcag cgcgaagagg cgcgcgaagt gtcgtttgaa
     5881 accgcactcg actcgcaact cggcgaagtg gcgacggatg aagtcttagt gctacagatt
     5941 ctcgacaacc tgttgacgaa cgcgctcgac gccgtcgaag ggcgcggttc gattcgtatt
     6001 accacgatgc tcgaaggcga gcgcacgcgc atcgaagtgg cggacacggg cgcgggcatt
     6061 ccggcggaga tgctcgtgcg catcttcgat ccgttctaca cgacgaaaga ggcgggcaaa
     6121 ggatcgggac tcggactcgc catctcggcc acgctcgccg aagctctggg cggcgcgctc
     6181 acggtcgagt ccaaacccgg cacgggcagc cgcttccgtc tctggctgcc gcgacgcgcg
     6241 cccgaaacaa gggatgaagg atgagggatg agggatgaat aagaaattga atgatttgtc
     6301 ttgaaatatt ggaagtgaga caaatgaaag aaaatgatct aagagtaaga acaaaagttt
     6361 tcgctctcag aattattaaa ctctatacgg ctttgccggg cactgttgag gcgcaagttc
     6421 tgggcaagca aatcctgcgc tcaggaacat cagtgggggc acattacaga gaagcatgtc
     6481 gagcaaaatc tgatgcggat ttcatcagca aaatcgaagg tggtttgcag gagctggaag
     6541 aaaccatcta ctggctggaa ctaattacag aatcaaaaat ctttcccgaa gaacgaatca
     6601 agcccctctg tgaagaatca gaagaactgc tcgccatgtt tgtgacaatg gttaagaatg
     6661 tgaaaggcaa gaaggagact cttacgcgat gacccaaata ttcttattca tccctcatcc
     6721 ctcatccttc atccctcaac acttatgact agaaagatca gtgtcctcgt cgttgatgat
     6781 gatccgctgt tgcgcaaact cgtgacggag cagttgtcgc gttccggttt cgagacggcg
     6841 gcggcggctt cgggcgagga ggccatcgaa gccttgcagg cgaccgatta cgatgtcgcc
     6901 ctcttagaca tccgcatgac cggcatgagc ggcctccacg ccctgcgcga gatcaggcga
     6961 ctcgaagacc cgcccgaagt gatcatgctc acggcggaca cttcgctcgg caccgggatc
     7021 gaagccatgc ggctcggcgc atacgactat ctcaccaagc cctcgacgct cgacgagatg
     7081 gaagccgtca ttcgcaaggc cgacgagaag cggcgactag tgagacagaa cgcaggcttg
     7141 cgcgcggcgg ccaatcgcgg cacgtctgcg gaagagaacg cgcacgcgct cat
//
LOCUS       DASWNS010000125         9772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_109399, whole genome shotgun sequence.
ACCESSION   DASWNS010000125 DASWNS010000000
VERSION     DASWNS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109399"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VGB76_04210"
     CDS             complement(<1..571)
                     /locus_tag="VGB76_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098141.1"
                     /translation="MLNSTKFRLAVIVGLLSSCAASLPAQENGRRNAPHARQVNKFAA
                     VSSPALQTLVDEAARETLQRFSDKKLAENQLAITLIDLRDAERPALASFRGEERIYTA
                     SVVKLFYLAAAHRWLEDGRLKETDELKRALRDMIVDSSNDATGYVLDVLTGTSSGVEL
                     PRAEMREWQEKRNAVNRYFASLGYTNINVN"
     gene            complement(586..1887)
                     /locus_tag="VGB76_04215"
     CDS             complement(586..1887)
                     /locus_tag="VGB76_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEY0098142.1"
                     /translation="MEFTVVEGLRTLKFDLVLTLALAALFLFVGGILQGRIGFLKRAS
                     IPAAVIGGLLFAGIVLALRSRGTLGIALDTTLRAPLQIAFFTTIGFGATLSLLGAGGW
                     RMVLFFVIASLAGVLQNVAGIGVALALGAPAALGIICGSLTLMGGPATGLAFTSQFEA
                     LGITGAGALIIASATFGIFVSSLVGNPVAAALIRRFRLAAPREETRRDETAEFWAIGS
                     TGGISDAREEEAEPHAQRREELPLAGGELLRHLLVLLVVMGFGALLGMGLSSLSVGGH
                     RLTLPAYVGAMVVAACVRNLDDRYGWFRLDMRAIEALGAIALALFLVIVLMDLKLWQL
                     AGLALPMLLILAVQVVLMIIYAVVVTFVVMGRDYEAAVTTSGHIGFGLGTTPNAVANM
                     EALTRRYGAAPRSFLIVPVVGAFFIDFSNALIITLFINFFR"
     gene            complement(1986..3686)
                     /locus_tag="VGB76_04220"
     CDS             complement(1986..3686)
                     /locus_tag="VGB76_04220"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0098143.1"
                     /translation="MKTKSLISLAVASCLLSLVAFNPHAGAQNMSADLVIVNANVHTL
                     DKARPQVEAVAVYGNRIAAVGTSVEIRKLAGAQTRIIDAQGALVLPGFNDAHVHFIDG
                     GSHLASVQLRDAASPEEFAERIRQFAAKTPKGRWIMGGDWDHERWPGAPLPTKELIDK
                     FTPDHPVFVNRLDGHMSLANSVALKLAGVTRETKDPDGGLIVRDAQTGEPTGVLKDAA
                     MSFVNAKIPAPTFDEKLDAARAATAHAASLGVTSVQDMSTGNDVGVLQTLAARGELKT
                     RVYGVSPLPQWERLARTGVRAVFGSDLLRVGGLKGFADGSLGSTTALFFEPYHDAPQM
                     RGLLGDEMFPEGEMQKRVEGADRAGLQVMIHAIGDSANDQILSLYERVTKSNGARDRR
                     FRIEHAQHIRPQDIARFGRGGVIASMQPYHAIDDGRWAEKRIGHERARTTYAFRTLLD
                     TGATLAFGTDWYVAPLNPMYGIYAASTRRTLDDKNPQGWIPEQKITVEEAVRAYTIGS
                     AYAEFAEQTKGTLAPGKLADLVILSQDIFRINPVEIERTKVRLTIMDGKVVYEEKVRG
                     "
     gene            3787..6642
                     /locus_tag="VGB76_04225"
     CDS             3787..6642
                     /locus_tag="VGB76_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0098144.1"
                     /translation="MDQLVENLRAVVGREHVRSDPDELLVYECDGLTQHKHTPRAVVF
                     PASTEEVSDVVKTLARAGVSFAPRGAGTGLSGGALAIERGVVVELARMRRLLKVDVEN
                     RLAVVETGMVNAGLSRAVAAHNLYYVPDPSSGAACTIGGNIAENAGGIHCLKYGTTTD
                     HVLAARVVLSDGQIVDLGNANGVPQPSGNALPGDALPRYDLLGVFIGSEGTFGIATEA
                     TVKLLPLPSAVRTMLADFTDVDAASRAVSAIIAAGLVPAALEMVDGATIRAVEASVFA
                     ANLPLDAEAALLIELDGLEAGLDAEAARAETLCREHGARHVRHAANEHERKRLWAARK
                     GAFGAMGRLAPDMMIQDAVVPRSRLPEVLAATYRIAAKYQLRLANVFHAGDGNLHPFI
                     SFDRRDGDEVLRVEVAGREIMETCVRVGGTITGEHGVGLDKSAYLPLIFSPDDLAAMM
                     QVRAAFDPSGLCNPGKIIPAPRGCGEARAVASGHATTAAAQATQTSRFEVRQPGLPPA
                     PGLDIDSTQKPSRSSSFALDGDGARRALAECVGEMNVSFKGETLVVAPANETEAREIM
                     RLASAHAWPVVPTGAHLRLDAFRSSCPLRLNLTTRRMTRMIRHEPADLVATAEAGLAL
                     AAFNQQLAQSDQWLPLDPPDDGRATLGGVAATGLSGALGMAYGAPRAFVLGMRVLLAD
                     GRMIKAGGRVVKNVAGYDLCKLFTGSHGTLGLITELTFKLRPRPACEATLIVRAPGAL
                     ALYRRARGLLAAEFLPLAFELLSPAFSAQLGLAADDGQFTALLRFAGAEETVASQLER
                     ARSLFAQDNLTSLEVVNGDATLWPALAAAPLRTTHELRWRAQVKPSDLPLLFDALARE
                     QSDPRVELLWHAGLGDGRLRVMQSAEAAGENGAARLNRLRRTVEENGGSLVLEAAPVE
                     IRREVTRRDRVEMEASLSRRVKQQLDPQGLLSQAIFL"
     gene            6732..8099
                     /locus_tag="VGB76_04230"
     CDS             6732..8099
                     /locus_tag="VGB76_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY0098145.1"
                     /translation="MTTKTPIQTAGALAAEENKLLACIHCGLCLEACPTYVHTGDEND
                     SPRGRIYLMRAVEEQRLSVESSSFERHINRCLGCRACESACPAGVEYGQLLEAARADL
                     ATQAGARRRGFSNRLLHFALRHIWLHPARLRFAFALSRVLRDAGVVRLLLRTRLPRLV
                     SSRLEFALALLQSSTPYKFETETQSSRPAPCDTKNDGRLAVKTRALAFEGCVTEGLFA
                     RVNRATARVLKANNCETSAPASQVCCGALHAHAGDLEGARKLARRNIEAFADTDGAHI
                     ITNAGGCGAMLLSYAHLLADDAQFAERARAFSERVRDVGQELELTGIRRGASPGATIT
                     TYDASCHLLHGQRAGDASLGMLRAIPALEFAPLTESETCCGGAGVYNLLEAELSTRVL
                     REKLSHLQATKAALVATGNPGCHMQIGAGAYLAGLSLHACHPVELLDESYRRAGVYEA
                     VASDE"
     gene            complement(8275..9486)
                     /gene="nagA"
                     /locus_tag="VGB76_04235"
     CDS             complement(8275..9486)
                     /gene="nagA"
                     /locus_tag="VGB76_04235"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEY0098146.1"
                     /translation="MTDSNPEHPPTLLRGARLVLPHGVVDDQNLLVERGRIARVIPAS
                     EAVPHAESFALDGLTIYPGFIDVHIHGAVGVDTMEATTDDLARVAQFLARSGVTAWLP
                     TLVPAPPEDYLRAANSIEHLIAAQDEDRTPAAARALGLHYEGPFINSAQCGALRPPYF
                     RTFKQPSDVSDLPVADAAGAIRMITVAPEIEGGIELVRELKARGWIVSIGHTRAGVET
                     LEAASAAGAQHMTHFFNAMSPLNHRTPGPVGWGLMHDEVTCDIIADGQHVAPLMLQLA
                     LRCKTAERLMLISDAVAPTGEGDGEFRIWNEKITVADGRTQNERGSIAGSVITMHDAV
                     RQMLALGVAPHEASQMASANPARLLGVAGSCGTIEEGKRADLAALDEAGRVRLTLIGG
                     RVAFDQTTLVE"
BASE COUNT         1769 a         3154 c         3061 g         1788 t
ORIGIN      
        1 ggttgacgtt gatgttggtg tagccgagcg acgcgaaata gcggttgacg gcgtttctct
       61 tctcctgcca ctcgcgcatt tcggcgcgcg gcaactccac cccgctcgaa gtgccggtca
      121 acacgtcgag cacgtagccg gtcgcatcgt ttgatgaatc gacgatcatg tctctgagcg
      181 cgcgtttcaa ctcgtcggtt tccttcaagc gtccgtcttc cagccagcga tgcgccgccg
      241 ccagatagaa caacttcacg acgctcgcag tgtagatgcg ctcctcgccg cgaaagctcg
      301 caagcgcggg gcgttccgcg tcgcgcagat caatcaaggt gatcgccagt tgattctccg
      361 cgagtttctt gtcgctgaaa cgctgcaagg tttcccgcgc ggcctcgtca accaacgtct
      421 gcaacgcggg cgacgagacg gcggcaaact tattgacctg ccttgcgtgg ggcgcgttcc
      481 tccgcccgtt ctcctgcgcc gggagactgg cggcgcaaga cgacaataaa ccgacgatga
      541 cggcgagacg aaatttcgtg ctattcaaca taagcaaact tatttttaac gaaagaagtt
      601 gatgaacaag gtgatgatga gcgcgttgga gaaatctatg aagaacgcgc cgaccacggg
      661 cacgatcaga aagctgcgcg gggctgcgcc gtagcgtcgc gttaaggctt ccatgttggc
      721 gacggcgttg ggcgtagtgc ccagaccgaa accgatatgg ccgcttgtcg tcaccgccgc
      781 ttcataatcg cgccccatca caacgaacgt cacgacgacg gcgtaaatta tcataagtac
      841 aacttgcacg gcgagtatca agagcatcgg caaggccagc cccgcgagtt gccacagttt
      901 caaatccatc agcacgatga cgagaaacag cgcgagcgca atcgcgccca gagcctcaat
      961 cgcacgcatg tcgagtcgaa accaaccgta gcgatcatct aaatttcgca cgcacgcggc
     1021 caccaccatc gcgcccacat aagccggaag cgtcaggcga tgccccccga ccgacaggct
     1081 cgataaaccc atccccagca gcgcgccgaa ccccatcacg acgagcagca cgagcaggtg
     1141 gcgcaacaac tcgccgcccg ccagcggcaa ctcttcccgg cgctgcgcgt gcggctcagc
     1201 ctcttcctcg cgcgcatcac taatcccgcc cgtcgagcca atcgcccaga actccgccgt
     1261 ctcgtctcgc ctcgtctcct cgcgcggcgc ggcgagacgg aagcgacgta tgagcgcggc
     1321 ggccaccgga tttcccacga gacttgagac gaagatgccg aacgtggccg aggcgataat
     1381 gagcgcgccc gcgcccgtga tgccgagagc ctcgaactgc gacgtgaacg ccaatcccgt
     1441 cgcggggcct cccatcagcg tcaacgaccc gcagatgatt ccgagcgcgg cgggcgcgcc
     1501 gagcgcgaga gccacgccga tccccgcgac gttttgcaac acccctgcca gcgacgcgat
     1561 gacgaagaac aagaccattc gccacccgcc cgcgccgagc agagagagcg tcgcgccgaa
     1621 accgatggtc gtgaagaaag cgatctgcaa aggggcgcgc aaggtagtgt cgagcgcaat
     1681 gccgagcgtg ccgcgcgaac gcaaggccag cacgattccc gcgaacaaca acccgccaat
     1741 caccgccgca gggatgctcg cgcgtttgag aaacccgatg cgcccctgca agatgccgcc
     1801 gacgaacaga aagagagccg ccagcgcgag cgtcaggacg agatcgaatt tcagcgtccg
     1861 cagaccttcg acgacagtga attccaacca gacctccaaa ctgagagagc agcgagcgaa
     1921 aggtcagagg ttagcagaag acaatttttc ccgacccctg acttctatcc tctgaactct
     1981 aaactctaac ccctgacttt ttcttcgtag acgactttgc cgtccatgat cgtcaggcga
     2041 actttcgttc gctcgatctc gacggggttg atgcggaaga tgtcttgcga gaggatcacg
     2101 aggtcggcga gtttgccggg cgcgagcgtg cctttggtct gctctgcgaa ctctgcgtag
     2161 gccgagccga tggtgtaggc gcggacagcc tcttccaccg tgatcttctg ttcgggtatc
     2221 cagccttgcg ggtttttatc gtcgagcgtg cggcgcgttg atgcggcgta gatgccatac
     2281 atcgggttga gcggcgcgac ataccagtct gtgccgaagg ccagcgtcgc tccggtgtcg
     2341 agcagcgtgc ggaaggcgta ggtcgtgcgt gcgcgttcgt ggccgatccg tttctccgcc
     2401 cagcgcccgt cgtcaatcgc gtgatagggc tgcatggaag cgatgacgcc gccgcgcccg
     2461 aagcgcgcga tgtcctgcgg gcgaatgtgt tgcgcgtgct cgatgcggaa gcgacggtcg
     2521 cgtgcgccgt tgctcttggt gacgcgttcg taaagagaga gtatttgatc gtttgcgctg
     2581 tcgccgatgg cgtgaatcat cacttgcaga cctgcgcggt ctgcgccctc cacgcgcttc
     2641 tgcatctcgc cttcgggaaa catctcgtcg ccgagcaggc cgcgcatctg cggcgcatca
     2701 tggtaaggct cgaagaagag cgcggtggtg gagccgaggc tgccgtctgc aaaacccttc
     2761 aacccgccca cgcgcaacag gtcgctgccg aacaccgcgc gcacgcccgt ccgcgccaga
     2821 cgttcccact gcggcagcgg cgagacgccg taaacgcgcg tcttcaactc gccgcgcgcc
     2881 gccagcgttt gcagaacgcc gacatcgttt cccgtggaca tgtcctgcac gctcgtgacg
     2941 ccgaggctcg ccgcgtgcgc cgtcgccgct cgcgccgcat cgagtttttc gtcaaaggtc
     3001 ggagcgggaa tcttcgcatt gacgaaactc atcgcggcgt cttttaacac gccggtcggc
     3061 tcgcccgtct gcgcatcgcg cacgatcaaa ccgccgtccg ggtctttcgt ctcacgcgtg
     3121 acgcccgcga gtttcagcgc gacgctgttg gcgagcgaca tgtggccgtc gagacggttg
     3181 acgaagacgg gatgatcggg cgtgaactta tcgatcaact ctttggtcgg caaaggcgcg
     3241 cccggccagc gttcatggtc ccaatcgcca cccatgatcc aacgcccctt cggagtcttc
     3301 gcggcgaact ggcggatgcg ctcggcaaac tcttcggggc tggcggcgtc acgcagttgc
     3361 acgctggcga gatggctgcc gccgtctatg aaatggacgt gcgcgtcgtt gaagccgggg
     3421 aggacgagtg cgccctgcgc gtcgatgatg cgcgtctgtg cgcccgcgag tttgcgtatc
     3481 tcgacgctcg tgccgacggc ggcgatgcgg ttgccataga cggcgacggc ttcaacctgc
     3541 ggtcgcgctt tgtccaacgt gtggacgttg gcgttgacga tgacgagatc agccgacata
     3601 ttttgtgcgc ccgcgtgcgg gttgaacgcg acgaggctca acaggcacga ggcgacggca
     3661 agcgaaatga gagatttggt tttcataaga tagaagagac atttgcgtgg catgcgggcg
     3721 cgtagtgtat cattgagggc gacctgtccg gtagaaaaat cattcgtcat cccagacagc
     3781 atttccgtgg atcaattagt cgaaaattta cgcgccgtcg tcgggcgcga gcacgtccgc
     3841 agcgacccgg acgaactgct cgtctatgaa tgcgacggcc tgacgcagca caagcacacg
     3901 ccgcgcgccg tggtcttccc cgcttcgacg gaagaagtct cggacgtggt taagacgctc
     3961 gcgcgtgcgg gcgtctcttt cgccccgcgc ggtgcgggga cgggactttc gggcggcgcg
     4021 ctcgccattg aacggggcgt cgtcgtcgaa ctcgcgcgga tgcgtcgcct gttgaaagtt
     4081 gacgttgaga atcgtctcgc cgtagtggag acgggcatgg tcaacgcagg tttgtcgcgc
     4141 gccgtcgccg cacacaactt gtattacgtc ccagaccctt cgagcggcgc ggcctgcacc
     4201 atcggcggca acatcgcgga gaacgcgggc ggcatccatt gtctgaagta cggcacgacg
     4261 accgaccatg tgctcgcggc gcgcgtcgtc ctgtccgacg ggcagatcgt tgatctcggc
     4321 aacgccaacg gcgtgcctca accttccggt aacgctctgc ccggtgatgc cctgccacgt
     4381 tatgatctgc ttggcgtctt catcggctcc gaaggaacgt tcggcatcgc cacggaggcg
     4441 acggtgaaac tcctgccctt gccttccgcc gtgcgcacga tgcttgcgga ctttacggac
     4501 gttgacgcgg cgagccgcgc cgtctccgcc atcatcgccg cagggcttgt gcccgccgcg
     4561 cttgagatgg ttgacggcgc aacgatccgc gcggtcgaag cgagcgtctt cgccgcgaat
     4621 ctcccgctcg acgcggaggc cgcgctgctc atcgaactgg acggactcga agccggtctt
     4681 gacgcggaag ccgcccgcgc cgaaacgctc tgccgcgagc acggcgcgcg acacgtccgg
     4741 cacgcggcca acgagcacga gcggaaaaga ttgtgggcgg cgcgtaaagg agccttcggc
     4801 gcgatgggtc ggcttgcgcc cgacatgatg attcaagacg cggtggtgcc gcgctcgcgt
     4861 ctgcccgaag tgctcgccgc cacgtatcgc atcgccgcga agtatcagtt gcggctcgcc
     4921 aacgtctttc acgcgggcga cggcaacctg cacccgttca tcagcttcga ccggcgcgac
     4981 ggcgacgaag tgttgcgcgt cgaagttgcc ggacgcgaga tcatggagac gtgcgtgcgc
     5041 gtaggcggca cgatcacggg cgagcacggc gtcgggctgg acaagagcgc gtacctgccg
     5101 ctcatctttt cgccggacga tctcgcggcc atgatgcagg tgcgcgccgc cttcgacccg
     5161 tcggggcttt gcaatcccgg caagatcatt cccgcaccgc gcggttgcgg agaagcccgc
     5221 gccgtcgctt ccgggcacgc aacgacggct gcggcgcaag ccacgcaaac atctcgcttt
     5281 gaagttagac agcccggcct cccgcccgcg ccggggttag atattgactc gacgcaaaag
     5341 ccttctcgct cctcttcatt cgcgctcgac ggcgacggcg cgcgacgcgc tctcgctgaa
     5401 tgcgtcggcg aaatgaacgt gtcgtttaaa ggggagacgc tcgtagttgc gcccgcgaat
     5461 gagacggaag cgcgcgagat catgcgtctc gcttcggcgc acgcgtggcc ggtcgtccct
     5521 acgggcgcgc atctccggct cgacgctttc cgatcctcat gcccgctacg tctcaatctc
     5581 acgacgcgac gcatgacccg catgatccgc cacgaacccg cagacctcgt ggcgaccgcc
     5641 gaagcgggac tcgcgctcgc cgctttcaat caacaactcg cgcagagcga ccaatggctg
     5701 ccgctcgacc cgcccgacga cgggcgcgcg acgctcggcg gcgtggccgc caccggactc
     5761 agcggcgcgc tcggcatggc ttatggtgcg ccgcgcgctt tcgtgctcgg catgcgcgtg
     5821 ctgctggccg acgggcgcat gatcaaagct ggcgggcgcg tcgtgaaaaa cgtcgcgggc
     5881 tacgatctct gtaaactttt cacgggcagc cacggcacgc tcggtctgat cacggaattg
     5941 actttcaagc tgcgcccgcg tcccgcctgc gaagcgactc tcatcgttcg cgcacccggc
     6001 gcgcttgccc tctaccgccg ggcgcgcggg ctgctcgccg cagagttttt gccgctcgcc
     6061 tttgaactac tctcgcctgc cttctccgcc caactcggtc tggcggcaga cgacgggcag
     6121 ttcaccgcac tccttcgctt cgcgggcgcg gaggaaaccg tcgcctccca acttgagcgc
     6181 gcgcgctccc tgttcgctca agacaacctt acgtcccttg aagtcgtcaa cggcgacgca
     6241 acactgtggc ctgcgctcgc cgccgcgcct ctacgcacta cccacgaact gcgctggcgc
     6301 gcgcaggtta aaccttccga cttgccgctt ctcttcgacg ccctcgcacg cgagcagagc
     6361 gacccacgcg ttgagctttt atggcatgcc ggattaggcg acgggcgttt gcgcgtgatg
     6421 cagagcgcgg aggctgccgg ggagaacggg gcggcgaggt tgaacaggct gcgccgcacg
     6481 gtcgaagaaa acggcgggtc gctcgttctt gaagccgcgc cggtcgagat cagacgggaa
     6541 gttacgaggc gagatcgtgt cgagatggag gcgtcgctgt cgaggcgcgt gaagcagcaa
     6601 ctcgatccgc agggtttgct ctcgcaagct atcttccttt gagcggcttc cttcgttttg
     6661 cgggcgcgcg gacaagcccc ggcctgagct agactgtctc cgcacgatga ttcgacacca
     6721 cacgcgattc gatgactact aaaactccaa ttcaaacggc gggtgcgctc gccgccgaag
     6781 agaataaact gctggcctgc attcattgcg gcctgtgcct cgaagcctgc ccgacctacg
     6841 tgcacacggg cgacgaaaac gatagcccgc gcggtcgcat ctacctgatg cgcgccgtcg
     6901 aggagcaacg cttgagcgtc gagtccagtt cgtttgaacg acacatcaat cgctgtctcg
     6961 gttgccgcgc atgcgagagc gcgtgtccgg cgggtgtcga gtacgggcaa ctgctggaag
     7021 cggcgcgcgc agacctggcg acgcaggcag gcgcgcggcg acgaggtttc tccaatcgcc
     7081 tgcttcattt cgcgctccgc cacatctggc tgcaccccgc acgcctgcgc ttcgccttcg
     7141 cgctctcgcg cgtgctgcgg gatgcgggcg tcgttcgcct gttgctccgc acaaggcttc
     7201 cgcgcctcgt ctcttcacgg ctcgaattcg ccctcgcgct tctgcaaagc tcaaccccgt
     7261 ataagtttga gactgagact caaagcagcc gcccggctcc gtgcgatacc aaaaacgacg
     7321 gacgccttgc agtgaagacg cgtgcgctcg ctttcgaggg ctgcgttacg gaaggactct
     7381 tcgcgcgtgt gaaccgtgcg accgcgcgcg tgctcaaggc gaacaactgc gagacgagcg
     7441 cgcccgcctc gcaagtctgc tgcggcgcgc tccatgctca cgcgggcgac ctcgaaggcg
     7501 cgcgcaagct cgcacggcgc aacatcgaag ccttcgcgga tacggacggc gcgcacatca
     7561 tcaccaacgc gggcggctgc ggcgcgatgc tcctctctta tgcgcacctg ctggcggacg
     7621 atgcgcagtt tgccgaacgg gcgcgagctt tcagcgagcg cgtgcgtgat gtcgggcagg
     7681 agttggaact gacgggcatc aggcgcggcg cgtcgcccgg cgcaaccatc acgacctatg
     7741 acgcttcgtg ccatcttctc cacggccagc gcgcgggaga tgcatcgctc ggaatgttgc
     7801 gcgccatccc tgcgcttgag tttgcgccgc tcacggagag cgaaacctgc tgcggcgggg
     7861 cgggcgtcta taacctgctc gaagcggaat tgtctacgcg cgtgctgcga gagaaactgt
     7921 cacacttgca ggccacgaaa gccgcgctcg tggcgacggg caatcccggc tgccacatgc
     7981 agatcggcgc gggcgcatac ctcgcaggtc tttccctcca cgcctgtcat cccgtagaac
     8041 tcctcgacga gtcctaccgg cgggcaggag tctatgaagc agtggcaagt gatgagtgat
     8101 gagtgacagg cgacgagtga cgggtgacaa gttaaaaaga ggtgagtagt gttgccgacg
     8161 ctcgtcgtgt cggccttccg ggcgtggggc tttcgggcga aacgcgcttg agaggttagg
     8221 cgtcgctggt ttcaaatctc tccgctcacc caccgctata gtttgctcgc gctgtcattc
     8281 cacaagtgtt gtttgatcaa atgccacgcg cccgccaatt aaggtcaacc gcacgcgtcc
     8341 cgcttcgtcg agcgcggcga ggtctgcgcg cttgccttct tcgattgtgc cgcaactccc
     8401 ggcaacgccg agcagccgcg cgggattggc cgatgccatt tgcgacgctt cgtgcggggc
     8461 gacgccgagc gcgagcatct gccgcacggc gtcgtgcatc gtgatgactg aaccggcgat
     8521 gctgccacgc tcgttctgcg tgcgcccgtc ggcgaccgtg atcttctcat tccagatgcg
     8581 aaactcgccg tcaccctcgc ccgtgggcgc aaccgcgtcg ctgatcagca tgagccgttc
     8641 ggcggtcttg cagcgcaggg cgagttggag catgagcggc gcgacgtgct ggccgtccgc
     8701 gatgatgtcg caagtcacct cgtcgtgcat gagtccccag ccgacgggac ccggcgtgcg
     8761 atgattgagc ggcgacatcg cgttgaagaa gtgcgtcatg tgctgcgcgc ccgccgccga
     8821 tgccgcctca agcgtctcga cacctgcgcg tgtgtgccct atggagacga tccagccgcg
     8881 cgcctttagt tcgcgcacca actcgatgcc gccttctatt tccggcgcga ccgtgatcat
     8941 ccgaatcgcc cccgccgcat cggcgacggg cagatcgctc acatccgacg gttgcttgaa
     9001 agtgcgaaag tagggcgggc gcagcgcgcc gcattgcgcg ctgttgatga aagggccttc
     9061 gtagtggagg ccgagcgcgc gggcagcagc gggcgtccgg tcttcgtcct gcgcggcgat
     9121 caaatgctcg attgagttgg ccgcacgaag gtaatcttcg gggggcgcgg gcacgagcgt
     9181 gggcagccat gccgtcacgc cggagcgcgc gaggaactgc gccacccgcg cgagatcatc
     9241 cgtcgtcgcc tccatcgtat ccacgccgac cgcgccgtga atgtgcacgt cgataaagcc
     9301 gggatagatg gtgaggccgt cgagagcgaa actctccgcg tgcggcacag cctcgctcgc
     9361 aggaataacg cgtgcgatgc ggccacgctc gacgagtaaa ttctgatcat ctacgacgcc
     9421 gtgcggaagc acgagtcgcg cgccgcgtaa gagtgtgggc ggatgttctg ggtttgaatc
     9481 agtcataact atcagcgcgt ttcaagaccc gcttcctgtt ctctttgcgc ttccagcgcg
     9541 tgattaagtt tcaggccgga ggcgcaggag gaaacagaag gtagttttgc gacgcgctct
     9601 aaagtaatat tcctttggcc gaaagctttc tcccgcgacg tttgacctga gagatactgc
     9661 acaacccttg atgagtcgcc tccgaataaa tctgcgttcg aagagcaacg attggcgcgg
     9721 tctgaagttt tgcgctgtcc tgtgcttttg ctggccgctc aatcttagct cg
//
LOCUS       DASWNS010000126         4248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_160280, whole genome shotgun sequence.
ACCESSION   DASWNS010000126 DASWNS010000000
VERSION     DASWNS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160280"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..1973
                     /locus_tag="VGB76_04240"
     CDS             393..1973
                     /locus_tag="VGB76_04240"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEY0098147.1"
                     /translation="MNTYLALFLIATITSLILTPIIRRVCQRCGWLDIPTDARRVHSA
                     AIPRLGGVAIFSSVVIALSLLLFRDNLITASLRTAEAKWLVLLLPTGLIFLVGVYDDL
                     KGMSAPFKFMAQGLAATLFYVLGGQINHLSVPSLGSVELPLAVSFIITVVWIIGISNA
                     FNLIDGIDGLAAGAALFASLVLMTVSLMLGHPQVTVVTVALCGALIGFLRYNFNPASI
                     FLGDSGSLTIGFLLAALSIQGAQKASTAVAVAIPLLAFSVPVFDTGFAIARRFISGKP
                     LFDGDREHVHHKLLERGWSQRRVVLVLYGVCALFGLLALLFVSDGSGRSIGFVLAVVA
                     VSVVFGVGRLRYHEVDEVKAGFKRNLGERRARVANNIQVRRASRAMSKATTLNDLLAA
                     VQQMLEHGEFVYASVQIGRGGDTATCERVLAAASPGLRGVDLRGGMICWSWERGDIEA
                     QKVIESHRFWTLRLPLFTNSAGWGYLTLYRGFDSEQLLLDMNYLYDLFQREMACAVER
                     VFELEKSELRAKSYAATS"
     gene            2088..3068
                     /locus_tag="VGB76_04245"
     CDS             2088..3068
                     /locus_tag="VGB76_04245"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY0098148.1"
                     /translation="MRILVTGGAGFIGSHLCERLVAGGHEVICLDNFFTGRRENITHL
                     LDNHRFELVRHDVIEPILLEVDQIYNLACPASPVHYQYNPVKTVKTNVMGTINMLGLA
                     KRVHARILQASTSEVYGDPEVHPQPESYWGSVNPIGIRSCYDEGKRLAETLMMDYHRQ
                     NGVDTRIARIFNTYGPRMLEHDGRVVSNFIVQSLRGEALTIYGTGEQTRSFCYVDDLV
                     EGLIRLMNVEPAGEEGSVNNPVNLGNPGEFTMRQLAEAVAELCGREVRIKYCPLPQDD
                     PRQRKPDITRALSLLGWSPNIPLREGLVKTIAYFAGRLEGALQEVESEGR"
     gene            3168..>4248
                     /locus_tag="VGB76_04250"
     CDS             3168..>4248
                     /locus_tag="VGB76_04250"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY0098149.1"
                     /translation="MNIAVIGTGYVGLVTGACFAEFGVNVTCVDIDAGKVACLNEGRV
                     PIYEPGLESLVQKNAQAGRLTFTTELKEAVEQSLVIFLAVGTPPKEDGSADLKYVDEA
                     VRTIAQAMNGYKVIVTKSTVPVGTGEHVRALIKRHQKRPVDFGVVSNPEFLREGAAIE
                     DFMRPDRIVIGSRDEAAVAIMRELYRPLYLIETPIVVTSLEAAELTKYAANAFLATKI
                     SFINEIANLCDRVGCDVYDVARAIGMDKRIGKSFLQPGPGYGGSCFPKDTLALLSVAG
                     QYGSESLIVGAAVEVNRRQRLSMVEKIERLAGNLEDKTLAVLGLSFKPQTDDMREAPA
                     VEIIRALMQRGARVRAYDPVAMEQAK"
BASE COUNT          840 a         1222 c         1261 g          925 t
ORIGIN      
        1 ctcataacac ggctccgcct ccctttattt ggaacactcg ccagacactc aggtaagtct
       61 gatagccaaa ctttcgtaca gttgctgttc cttctctgaa cttcatcttc ggggtgatga
      121 cgatccggag agtcccggac acatgttgag gcttgcgccg caggtagcgc ggccagcgcg
      181 ttgctctaac tctgagagga cggataaaag ggcactcgaa cctttttgtc agctcggcgc
      241 gactctctcc aattctcaat ctcacatcaa ctcttttgtg ggtcgtagag atcaggaacg
      301 tctccaatct gattgatggc gttgaaccgt cggacagtac tcggatgtct ctcacgtacg
      361 tgcggcagtg acttgcagaa agaagctctc ccatgaatac ctatctagcg ctattcctca
      421 tagccaccat cacctcattg attctgacgc ccattattcg ccgtgtctgc cagcgttgcg
      481 gatggttgga tatccctaca gatgcgaggc gcgttcatag cgccgcgata ccgcggctcg
      541 gcggcgtggc gatcttcagc tccgtcgtga ttgctctctc tttacttctt ttccgggata
      601 acctgataac ggcctctctg cgcactgcgg aggctaagtg gctggtgctt ttactcccca
      661 cagggttgat ctttctggtc ggcgtttatg atgatcttaa aggaatgagc gcgcccttca
      721 agttcatggc acaaggattg gccgccacgc tcttctacgt gttaggcggg cagattaacc
      781 atttatcagt accgtctctg ggctccgtcg aactcccact cgccgtcagc ttcatcatta
      841 ccgtcgtctg gatcatcggg atcagcaatg ccttcaacct gattgacggg atagacgggc
      901 tggcggcggg cgcggctctc ttcgcctcgc tcgtgttgat gactgtctcg ctcatgttag
      961 gccacccgca ggtgaccgtc gtgaccgtcg ctctgtgcgg cgcgctcata ggatttctgc
     1021 gttataactt caatccggcc tcaatctttt taggcgactc gggttcctta actatcggat
     1081 tcctcttggc cgctctctcc attcagggag cgcaaaaggc ttcgacagca gttgccgtcg
     1141 ccatcccctt gctggcgttc agtgtgccgg tcttcgatac cggcttcgct attgcgcgcc
     1201 gcttcattag cgggaagcca ttgtttgatg gtgaccggga gcacgtccat cacaagctgc
     1261 tggagcgcgg ctggtcgcaa cgcagggtgg tgctcgtgct ctacggcgtg tgcgccctct
     1321 tcggcctgct ggctctcctc ttcgtcagcg acgggagtgg acgctccata ggttttgtgc
     1381 tcgcggtggt cgcggtttcc gtcgttttcg gagttggccg ccttcgctat cacgaggtgg
     1441 acgaggtcaa agcaggattt aaacgtaatc tgggcgagcg tcgcgcacgc gtagccaata
     1501 acattcaagt gcgtcgcgcg agccgcgcca tgtcgaaggc cacaaccctc aacgacctct
     1561 tagccgccgt gcagcagatg ctcgaacatg gtgaattcgt ctatgcttct gttcagatag
     1621 gaaggggcgg cgatacggcc acctgcgagc gtgtacttgc ggctgcgtcc ccgggtcttc
     1681 gtggcgttga ccttcgcggc gggatgatct gttggtcttg ggagcgcggc gatatcgaag
     1741 cgcagaaggt catcgaatca caccgctttt ggactctccg cctgccactc ttcaccaaca
     1801 gtgccgggtg gggctacctg acgctgtatc gcggctttga tagcgagcag ttgctgcttg
     1861 atatgaacta tctgtacgac cttttccagc gtgagatggc ttgtgcggtt gagagagtat
     1921 tcgagctgga aaaatccgag ctgcgggcga aaagctatgc cgcaacaagt taagaattag
     1981 cgcataatag agttgtcggg tctaatcggt gctgaacagg gtgagcctgt ccttaccttg
     2041 ttgcacctct gttaaaggct gctgaataac tttagagaga cgagaatatg agaatactgg
     2101 taacgggagg agccggattc atcggctcgc acctctgcga gaggctggtg gcgggagggc
     2161 atgaagtgat ctgcctggac aacttcttta ctggcaggcg cgagaacatc acgcacctgc
     2221 tggataacca ccgcttcgaa ctcgtcaggc acgacgtgat agaacccatc ctcctggagg
     2281 tagatcagat ctacaacctg gcttgcccgg cttctcccgt tcactaccag tacaatcctg
     2341 tcaagacggt caaaaccaac gtcatgggca cgattaacat gctcggcctt gccaagcgcg
     2401 tgcatgcgcg catccttcag gcctcgactt ccgaagtcta cggcgacccg gaagtgcacc
     2461 cgcagccgga gagctactgg ggaagcgtca accccatcgg gatcagatca tgctatgacg
     2521 agggcaagcg gctcgccgaa acgttgatga tggactatca ccggcagaac ggcgtggaca
     2581 cgcgcatcgc ccggatattt aacacctacg gtccgcgtat gctcgaacac gacgggcgcg
     2641 tggtgtcgaa cttcatcgtg caatccctga ggggggaagc gttgacgatc tacgggaccg
     2701 gcgagcagac acgctcgttc tgttacgtgg atgacctcgt cgaaggattg atccgcctga
     2761 tgaacgtcga accggcgggc gaggagggaa gcgtcaataa ccccgtaaac ctcggcaacc
     2821 cgggcgagtt tacgatgcgg caactggccg aagcggtggc cgagttgtgc ggacgtgagg
     2881 tgcgtatcaa gtactgtccg ctcccccaag acgatccgcg ccagcggaag cctgatatca
     2941 cgcgcgctct gagcctgctc gggtggtcgc ccaacatccc tctgcgggaa ggactggtca
     3001 agacgatcgc gtatttcgcg gggcgtctgg aaggcgcgtt gcaggaagta gagtcagaag
     3061 gccgctagcc ggtacggcta ctcacgccag acccgacgcc ggtaggctgt ttcgtccagc
     3121 cgggttttcc tcatttaatt cagcgagaaa tacagaaaaa tctttatatg aacatcgcag
     3181 tgattgggac gggctacgtc ggattggtaa ccggagcctg cttcgccgag ttcggcgtca
     3241 acgtcacctg cgttgacatc gacgcgggga aggtcgcgtg ccttaacgaa gggcgggttc
     3301 ctatctacga gccagggctt gagtcgctcg tccagaagaa cgcgcaggcc gggcggctca
     3361 ccttcacgac cgaactcaag gaggcggtcg agcaatcgct cgtcatcttc ctcgccgtcg
     3421 gcacgcctcc gaaagaggac ggatcagccg acttgaagta cgtggacgag gccgtgcgca
     3481 ccatcgctca ggcgatgaac gggtacaaag tcatcgtcac gaaatcgacg gtcccggtcg
     3541 gcacgggcga gcacgtccgt gcactcatca agcgacacca gaagaggcca gtggatttcg
     3601 gcgtggtgtc aaacccggag ttcttgcgcg agggcgcggc tatcgaagac ttcatgcgtc
     3661 ccgaccgcat cgttatcggc agccgggacg aggccgccgt cgccatcatg cgcgaactct
     3721 accgcccgct ctacctcatc gagacgccca tcgtcgtcac ctcgctcgaa gcggcggaac
     3781 tgaccaagta cgcggccaac gcttttctgg cgacgaagat ctccttcatc aacgagatcg
     3841 cgaatctgtg cgaccgcgtg ggatgcgatg tttacgacgt ggcgcgcgcc atcgggatgg
     3901 acaagcgcat cggaaagagc tttttgcagc cgggtcccgg gtacggcggg tcgtgcttcc
     3961 cgaaggacac tttggcgttg ctgtctgtcg ccgggcagta cggctccgaa tcgctcatcg
     4021 tcggggcggc ggtcgaagtc aatcgcaggc agcgtctgtc gatggtcgag aagatcgagc
     4081 ggctcgcggg aaacctggag gacaagacgc tcgccgtgct cgggctgtcc ttcaaacctc
     4141 agacggacga catgcgcgaa gctccggcgg tggagatcat ccgtgctctc atgcagcgcg
     4201 gcgcgcgcgt ccgcgcttac gacccggtgg cgatggaaca ggcgaagc
//
LOCUS       DASWNS010000127         4622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_187569, whole genome shotgun sequence.
ACCESSION   DASWNS010000127 DASWNS010000000
VERSION     DASWNS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187569"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..2976)
                     /gene="ppc"
                     /locus_tag="VGB76_04255"
     CDS             complement(127..2976)
                     /gene="ppc"
                     /locus_tag="VGB76_04255"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY0098150.1"
                     /translation="MAKSLWQVDDQSARLAELTSGDEGVKELPLRRDVRSLGQLLGQK
                     LKEQGGESLYNSVELLRRLTSEHRDTNAGVADVAAVQSSTPEDVEPMARAEAVVKRLN
                     VLEAYQLTKAFAIYFELTNLAETNHRKQRRRARTLAANLPALPGTFRGTLERLREAGI
                     AWQDALAVLRRIEVAPVFTAHPTEVARRTILFKRRRIADGLERLSRLPLSDAEAAAAE
                     AAIEREITALWQTDDIRRRQPTVEDEIMMGLDYYPSVLIDTLPEVYAEMASGFAQVYD
                     ARVEAHELPNVISFASWIGGDRDGNPYVTAATTREALLLARHTILDYYLKAVDHLQEQ
                     LSLSAHQVKASTRLREKFESYSETLAGAQEEVSRHPPAEIYRRFLAYVLYRLRSTREA
                     PAGVHGYAEAAAFSSDLLIVRESLCENVGGELAEGLLDPLLRQAETFGFHLHTLDIRQ
                     HARVHAHAVRELAGGLRFDGEASAAQSSAEGQATGEIPAVSTETAALLDSLRAVAELK
                     RTFAPQAIRAYVISGASAGADVLALLWLMELCGVKAGATRERDDPGVMPVPLFESIED
                     LRRAPEVCRRLWTSPLYARVLDSWGREQEIMLGYSDSNKDGGMITSTWEIYKAHDELH
                     RVAHECDVRLSLFHGRGGTVGRGGGPTHRAIAAQPPGAFTGRIRITEQGEVLNFKYAD
                     AVLAERSFELMIAASLDTLARPGAFPVEVAWTEALEEMSAEAFRFYRESVVENASTLR
                     YFQQATPVREFELAKSGSRPSRRGEEGKAPGDPRDEIASLRAIPWVFGWMQSRHVLPA
                     WFGVGHALSLYCRAHEGGEELLRTMMGKFPLFHDLISNVENGLAKADLSIARRYSLLV
                     EDEELRERVWRMIAEEFALTREMILKVTGQKELLENNPVLTRSIRLRNPYVDPMSLIQ
                     IELLRRKRGGEESEELNYALAATINGISAGLRNTG"
     gene            3792..>4622
                     /locus_tag="VGB76_04260"
     CDS             3792..>4622
                     /locus_tag="VGB76_04260"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0098151.1"
                     /translation="MCKYVFRQSVVAVMLVLSLFAVASAQEFRGSLSGNVSDPNGAIL
                     PGATVELRNVETNVATTVVTNEDGGFSFPLLQPGMYTLSVTSQGFTTARREGIEIRVA
                     DRLTLDVQMQTGVAETVNVVDSTVALETGSVSAGTVIEQKQISELPLIDGSPYQLATL
                     APGISYTGNPAFTAPTSNGNLASFRTNGATGPNQVTLDGSPNFAIDGAVGFSPPSDAV
                     SQFKVQTTQFDAQQGYTANATVNVALKSGTNDFHGALYYFNRDKSRAANNFFSNAAGQ
                     E"
BASE COUNT          878 a         1470 c         1363 g          911 t
ORIGIN      
        1 ccgatcaaaa agtagagaga taattccgtc tggcagtccg gttgacaggc gtttgcgacg
       61 gacgcgcaat cacgtcgggt ttgatctttt gaactctaca ggttgcttat ccgaaccttg
      121 agaagtctaa cccgtgttac gcagacccgc cgagatgccg ttgatggtcg cggcgagcgc
      181 gtagttcaac tcttcgctct cctcgccgcc gcgcttgcgt cgcagcagtt cgatctggat
      241 cagactcatc ggatcgacgt aaggattgcg caggcggatc gagcgcgtga ggacagggtt
      301 gttctccaac aactccttct gtccggtaac ctttaagatc atctctcgcg tgagcgcgaa
      361 ctcttccgcg atcatccgcc acacgcgctc tctcaactcc tcatcctcaa ccagcagact
      421 gtagcggcgt gcaatcgaga gatcggcttt ggcaagaccg ttctcgacgt tgctgatgag
      481 gtcgtgaaag agtggaaact tccccatcat cgtgcggagc aactcttcgc cgccctcgtg
      541 cgcgcggcaa taaagcgaga gcgcgtggcc gacgccgaac cacgcgggca gcacatggcg
      601 actctgcatc cagccgaaga cccaggggat cgcgcgcagg ctggctatct cgtctcgcgg
      661 atcgcccgga gcctttccct cttccccgcg tcggctcgga cgcgagccgc tcttcgcaag
      721 ttcaaattcc cgcaccggcg tcgcctgctg aaagtatcgg agcgtggacg cgttctccac
      781 cacagactct ctgtaaaatc tgaacgcctc agccgacatc tcttccaacg cttccgtcca
      841 tgcgacttcg acgggaaaag caccggggcg cgcgagcgtg tcgagcgagg cggcgatcat
      901 cagttcaaaa ctgcgttcgg ccaagaccgc atcggcatat ttgaagttca ggacttcgcc
      961 ttgctccgtg atcctgatcc tgccggtgaa ggctccgggc ggttgggcgg cgatggcgcg
     1021 atgggttgga ccgccgccgc gcccgaccgt gccgccgcga ccgtgaaaga ggctcaaacg
     1081 cacgtcgcac tcgtgcgcca cgcgatgcaa ttcgtcgtgc gctttgtaaa tttcccaggt
     1141 gctcgtgatc atcccgccgt ctttgttcga gtcggaatag ccgagcatga tctcctgctc
     1201 gcgcccccac gaatcgagga cgcgcgcgta gagcggcgaa gtccagagac ggcggcagac
     1261 ttccggcgcg cgccgcaagt cttcaattga ctcgaagagc ggcacgggca tcacgcccgg
     1321 atcatcgcgt tcacgcgttg ccccggcctt cacgccgcac agttccatga gccagaggag
     1381 cgcgagcacg tctgcgcccg cgctcgcgcc gctgatgacg taggcgcgta tggcctgcgg
     1441 cgcaaacgtc cgcttcagtt cggcgacggc gcgcaggcta tcgaggagcg cggcggtttc
     1501 tgtcgaaacg gctggaatct ctccggtcgc ctgcccttcg gctgacgact gcgcggcaga
     1561 cgcttctccg tcgaagcgca agccgcccgc gagttcacgt acggcgtgtg cgtggacgcg
     1621 cgcgtgctgt cgtatgtcga gcgtgtggag gtgaaagccg aacgtctccg cttgacgcag
     1681 cagcggatcg agcagccctt cggccaactc gccgccgacg ttctcacaga ggctctcgcg
     1741 cacgatcaat agatcggagg agaaggcggc ggcctccgcg tagccgtgga cgcctgccgg
     1801 ggcttcgcgc gtcgagcgca ggcggtagag cacgtaagcg aggaagcggc gatagatttc
     1861 ggcgggcggg tggcggctga cctcctcctg cgctcccgcc agagtctcag agtaactttc
     1921 aaacttctct cgtaaacgcg tggaagcctt cacctgatgc gccgagagac tgagttgctc
     1981 ctgcaagtga tcgacggctt tcaggtagta gtcgagaatc gtgtggcgcg cgaggaggag
     2041 agcttcgcgg gtggtggcgg cagtcacata cgggttgccg tcgcggtcgc cgccgatcca
     2101 ggaggcgaag ctgatcacgt tcggcaattc atgagcttcc acgcgtgcgt cgtaaacctg
     2161 tgcaaacccg ctcgccatct ccgcgtagac ttcgggcagc gtgtcgatca ggacggaagg
     2221 gtaatagtcc agccccatca tgatctcgtc ttcgacggtc ggctggcggc gtcggatgtc
     2281 gtccgtctgc cagagcgcgg tgatctcgcg ctcgatggcg gcctcggcgg cagcggcctc
     2341 cgcgtctgag agaggcaggc ggctcaggcg ttcgagcccg tctgcgatgc ggcgacgttt
     2401 gaagaggatc gtgcgacggg ctacttccgt cgggtgcgcc gtgaagacgg gcgcgacctc
     2461 gatccgcctt aagacagcga gcgcgtcctg ccatgcgatg cccgcctcgc gcagacgctc
     2521 cagcgtgccg cgaaaagtgc cggggagcgc gggcaggttt gcggcgagcg tgcgcgcgcg
     2581 cctgcgttgc ttgcgatgat tggtttcggc gagattggtg agttcgaaat agatcgcgaa
     2641 ggctttggtc agttggtacg cctcaaggac gttcaagcgt ttgacgaccg cttcggctcg
     2701 cgccatcggc tcgacatctt ccggcgtcga actctgcacg gcagcaacgt ctgcgactcc
     2761 cgcgttcgtg tctcgatgct cgctcgtcaa acggcgcagc aactccacgc tgttgtagag
     2821 cgattccccg ccctgctcct tgagcttctg ccccagcagt tggccgagcg agcgcacatc
     2881 gcgccgcagg ggaagttcct tcaccccctc gtcgccgctc gtcaactccg ccagccgcgc
     2941 cgactgatca tccacctgcc acaaagactt cgccactttt cgcacccgct ccgccgcctg
     3001 tcaattttca tcccggcgca aagattcgtt caaacctttg cgctgcccct caagccttcg
     3061 cgccctgccg ggcactgttg cagaaattgc ctacgggctt ttatcctaac tcagtttcaa
     3121 ctatcgcgct tcgtgaatca tactcggaaa caacataaat ccttcttcca gcaacgccgc
     3181 ccgcgcaaca ttactctttt acgcgctact cgacgccgcg ccgacagcgt tctcagcgca
     3241 tagctctccc ctgcgcggga ggcacatccg acaagatacc gcacggtcta ttcggacgat
     3301 tcggaggccg cccatctctt tgttaattgg tcagaccctt caatgtttag aaatataatt
     3361 ggttaaaaat gaacttgaca aaaattttcg cctgatagaa aatgcccacg cttcttcaat
     3421 gctgtgtaac aggcgcgatt gcgagactcg cagccgcccg cgaaagctcg aacaagcaac
     3481 taattcttgc ctctgagaga ttttttgaag caaactgccg aatcgcccgt gtctctgaag
     3541 ccgtcgccgc gcgggttctc gcgtgggcgt tcgcaatttt cagcggcgaa cgccacttaa
     3601 gattaattgg ttacaccagt attcgtatac tgcctgattt ggcacgctga attcgcagcc
     3661 tgaataaaaa caggcacacc aggttccagt cccgcttgaa taatcgacgc tgtactctag
     3721 ggtcgcagca aggatgtaca aggcggcgac gccactcatc tggaagtaaa gtttagaaag
     3781 gaatagagtg tatgtgtaag tacgtattcc gccagagcgt tgtcgccgtc atgctggtgc
     3841 tctcgctctt tgcggtcgcc agcgcgcagg agtttcgcgg ttcgctctcc ggcaacgtct
     3901 cagacccgaa cggggcgatc ttgccgggtg ctacggtcga actgaggaac gtggaaacga
     3961 acgtcgccac cacggtcgtc accaacgaag acggcggctt cagtttcccg ctgcttcaac
     4021 ccggcatgta tacgctgtcg gtgacgagtc agggcttcac gacggcgcgg cgcgaaggca
     4081 tcgagatacg cgtggccgac aggctgacgc tggatgttca gatgcagacc ggcgtggccg
     4141 agactgtgaa cgtcgttgat tcgaccgtcg cgctcgaaac cgggtcggtc agcgcaggca
     4201 ccgtgatcga gcagaagcag atcagcgagt tgcccttgat cgacggcagc ccgtaccagt
     4261 tagcgacgct cgctccgggc atctcttaca cgggcaaccc ggccttcacc gcgccgacct
     4321 cgaacggcaa cctcgcctcg ttccgcacga acggcgcaac cggccccaat caggtcacgc
     4381 tcgacggctc gcccaacttc gccatcgacg gcgcggtcgg cttctccccg ccctcggatg
     4441 ccgtctcgca gttcaaagtg cagaccacgc agttcgacgc gcagcagggc tacacggcca
     4501 atgcgaccgt caacgtcgcc ctcaagtccg ggacgaacga cttccacggc gcgctctact
     4561 acttcaaccg cgacaagagc cgcgcagcca acaacttctt ctccaacgcc gccgggcagg
     4621 ag
//
LOCUS       DASWNS010000128         8156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_290771, whole genome shotgun sequence.
ACCESSION   DASWNS010000128 DASWNS010000000
VERSION     DASWNS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290771"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..333)
                     /locus_tag="VGB76_04265"
     CDS             complement(28..333)
                     /locus_tag="VGB76_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098152.1"
                     /translation="MERVQITVVEDAFPLAGRGIIGTGIWLDEAQEIPNNALVEVVCP
                     GGTTFRTSLGGVTLFTKCFSEKRVIGLLFGSLSDKSQIPLGSEVWLVESLDNSLDRT"
     gene            complement(456..1055)
                     /locus_tag="VGB76_04270"
     CDS             complement(456..1055)
                     /locus_tag="VGB76_04270"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY0098153.1"
                     /translation="MSSFLGHGLAAYSLFSLDRRKWPSTRRRAMWLGWLVVLASAPDI
                     DYLVPALGSPAHQGSRITHSVAFSLILPLSSVGVLYFVQGLKSQRRLLSICAVLAGLS
                     HLVLDFLVGVTPLPLLWPLGSTPFVSPVGILPSAGRIQLSNYYLYRNVLIEVGVLAPL
                     FYVARELYLGERGARNRARMLILLLVAGCFVMWSVSLSR"
     gene            1783..2232
                     /locus_tag="VGB76_04275"
     CDS             1783..2232
                     /locus_tag="VGB76_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0098154.1"
                     /translation="MRPEEVCRLRRENVHLDHGYLFNPYGKTKAAKRKVPLSDKAVAI
                     LSKRLADVKGVYLFPGRGVGDAPIVKVNNAHTATLGRSGVRPFRLYDLRHTWATRAAM
                     ANVDLVTLAAMLGHSRVQMVMRYAHPTEEHQFNAMKKVQTFVAGGLK"
     gene            complement(2270..4192)
                     /locus_tag="VGB76_04280"
     CDS             complement(2270..4192)
                     /locus_tag="VGB76_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098155.1"
                     /translation="MTLAETLLRQLENCALSRNERAQLQCKIAADFEHRGEYESARDA
                     LGELWQGVGERPTLDGLSELTAAEVLLRVGSLSGWLGSGRQIKGVQEKAKDLISESIT
                     RFQTLGETTKVVAARSELGFCYRRAGAYDDARVVYQEALRELGDGSEKELRAKILLRL
                     AIVESLSGRYNDSLRILVDEARFFEESTNDFLKGKFHNDLACLLVCLSKAERRPDYID
                     RAIIEYTAASVHFERADHTSYFARAENNLGFLLHTVGRYEEAHQHLNRARHLFATVSD
                     QGSVAQVDETRARVLLAQDRLRAAESAIREAVRVLSKGGEQGLLAEALTTQGRILSKR
                     GNFIESLNTLRRAADLAEEAGAVEDAGRALLSLMEEHADHLNEHELLEAYERAHNLLK
                     ETQDAETISRLRACAIRIVSDRRVSLSQRRVRSVVDFWANFDLNERVRTYEARYVRRA
                     LIDGGGSVTRAARLLGLRHHATLTYMLDAEQGRHKDLAYLRTPPEPRKQSIINRNSRR
                     GRPRNKARTINILCVEDYQVVADAVKETLEELGWTVELCADGTEAIRKIESKARYHLL
                     ILDNQLPGKDGLELARRARQLPHRRRTPIIMLSASDVEQDALRAGVNAFLRKPQDIGR
                     LSAMVTRLLTKNTAAK"
     gene            4326..4550
                     /locus_tag="VGB76_04285"
     CDS             4326..4550
                     /locus_tag="VGB76_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098156.1"
                     /translation="MKSYRNAIAALVLAFVFSTSAFAGDGIMWTDKTPPPPPPQADGI
                     MWTDVASPTSEGDALTEIALTLLQTLIPLL"
     gene            complement(4866..4937)
                     /locus_tag="VGB76_04290"
     tRNA            complement(4866..4937)
                     /locus_tag="VGB76_04290"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4903..4905),aa:Cys,seq:gca)
     gene            complement(5021..5095)
                     /locus_tag="VGB76_04295"
     tRNA            complement(5021..5095)
                     /locus_tag="VGB76_04295"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5061..5063),aa:Gly,seq:gcc)
     gene            5377..7845
                     /locus_tag="VGB76_04300"
     CDS             5377..7845
                     /locus_tag="VGB76_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0098157.1"
                     /translation="MRSTLSLCALLLLLASVVAAQGYRKPPQAVLDVLNSPVTPSISI
                     SPARDQLLLATGVRYPPINDLAQPMLRLAGYRINPNTNGRHLAPYFVALSLKRIADGA
                     ETKITLPPGAKAGFPQWSPDGRHFAFTNTIANGIELWVGDAASGRVTKIKNVLVNAAY
                     GDPLVWMPDNRTLLAQLVPAARARLAPAPDVPREPNVQESSGRAGPVRTYQDLLKTPY
                     DEELFDYYATAQLALIDVASGRVTNINQPAIYENAAPAPDGQHLLVTRLKRPYSYLHP
                     ANDFPKDVEVWDTRGKLIHKLASQPLQDQVPIEGVPTGPRAHSWRPTEPATIVWAEAL
                     DEGDPKKKVTHRDRLLISKAPFTGAPTELMKLEDRYAGIQWLERGGLAFASDYNRDTR
                     RVRTQLVSVDNASVAPRVVWNRNVQDRYNDPGSPITRILPTGHRVIIYNNDQIYLSGA
                     GASNAGDRPFLDRFNLTTLKGERIFRSDEKSYEAFVALLKDDASQFITRRETPIEPGN
                     FFIRTLGGAAGTAATGEAAMTSTSKPLTQFPDPTPQLRDIKKQLVTYKRADGVQLSFT
                     LYLPPGYKEGTRLPTVVWAYPLEFNDAATAGQVSGSTQRFTTIGGPSHLFFLLQGYAV
                     LDNTTMPVVGDPETVNNTYVEQINESAKAAIQKAADMGVTDPERVGVGGHSYGAFMTA
                     NLLAHSDLFRAGIARSGAYNRTLTPFGFQSERRILWEAPDMYIKVSPFMNAHKIKEPV
                     LLIHGEADNNTGTFPIQSERMYQAIRGTGGTVRLVMLPLESHGYSARETTEHVLFEML
                     NWFDKYVKNAPPRTQEQKASASTK"
     gene            complement(7961..>8156)
                     /locus_tag="VGB76_04305"
     CDS             complement(7961..>8156)
                     /locus_tag="VGB76_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098158.1"
                     /translation="TRAARAYRRTFNLANIPYRYDVVSVILPHATVEKPAPNPRLELL
                     RNFWTEAKFRKRHWSDANSP"
BASE COUNT         1785 a         2482 c         2198 g         1691 t
ORIGIN      
        1 cgcctcattg cgggcgcgtg cctcgcgcta cgtccggtcg agcgagttgt caaggctctc
       61 gactaaccac acctcagagc ctaaaggaat ttgggacttg tccgaaaggc tgccgaacag
      121 aagtccaatc acgcgtttct cgctgaagca ttttgtaaaa agagtgacgc ccccgaggct
      181 ggttctgaat gtcgtgccgc cgggacagac aacctcgacc agagcattgt tcggaatctc
      241 ctgtgcctcg tccagccaga ttccggtccc aatgatgccg cgccccgcta gcgggaacgc
      301 atcctccaca acggtaatct gcactctttc cataagaaac ctaacgcgcg aattgaccgg
      361 cgagctatca aaccggatga taaaaatcat cctaattgcg agtccgtttg aaggagttga
      421 tagacgtgcc ggtgcccgca atgacgcgcc gcttttcacc tgctcaaact tactgaccac
      481 atcacaaaac atcccgcgac cagcaatagt attaacatcc ttgctctgtt ccgggcaccc
      541 ctctcgccca gatataattc ccgcgcaacg taaaagagcg gcgccagcac gcctacctcg
      601 atgagcacgt tccggtataa ataatagttc gataattgta tccggcctgc actcggcaag
      661 atgccgaccg gggaaacgaa tggcgtcgag ccgagcggcc agagcagcgg tagcggtgtc
      721 accccgacca gaaaatccag gactaagtgc gacaacccgg ccagtacggc gcaaatgctc
      781 aataacctgc gttgactttt tagaccctgc acgaagtaaa gcacgccgac gcttgatagc
      841 ggcaggatca ggctgaaggc gacggagtgc gtgatcctgc tcccctgatg agcgggcgag
      901 ccgagcgcgg gaacgaggta atcgatgtcg ggcgcggacg cgagcactac cagccatccg
      961 agccacatcg ccctccggcg tgtggaaggc cacttccgtc ggtctaggga gaagagcgag
     1021 taagccgcta aaccgtgtcc gaggaacgat gacataaatt agaaccggtg actcttttag
     1081 atgataaccg cttaaccccc cggcatcacc cgctgcgtat tcaacctcac aggcacggcg
     1141 tctcgcggat gatggccgca ttatctgcgg tcgggtgcgt gccgatgtca ggctgcgcct
     1201 cgccataggc cacgccgcgc tgcgcgagcc tcatcctccg ctcgcgcata atggcattgg
     1261 ctataactgg acatgctgta aggctctggt tgcttatgct gtgccatccc cgactgaatc
     1321 ataagcaggt tcacgtctag catctccata tttcgtaaat gtacgacacc cgatattgcc
     1381 acagtggatg gatgacgctt ctgacgcgct tcccatgcac gcgcagggtt tacccaaacc
     1441 tcaaccacct taccccgcac catgcgttcc agtaaccggt gcgctgcccg agcaaaccgc
     1501 tggctgccct caggggcagc aatgccgacc atctgtacgc gccgcaggcc gctaccctta
     1561 acacttatga tgactgtatc cccatcttca actttaacga ccctcccgtg gacgctaccc
     1621 catgactgac tctcaactgc ggcatcgcca cacgggtcag ccggttcggc atgttgagcc
     1681 aacacaatct ggttgtaaga cagcaggaga caataaagac caatccgcat aaatattttc
     1741 atagattctc aaaccttagc agcatgacgc ccccatgaag ggatgcgccc cgaagaagtt
     1801 tgccgtcttc ggcgcgagaa tgtacacctt gatcacggct atctattcaa cccttacggc
     1861 aagactaaag cggcaaagcg taaagtcccg ctctccgaca aagctgtcgc tatcctttca
     1921 aagcgtctcg ccgacgtgaa aggcgtatat ctctttcccg gtcgcggcgt tggcgatgcg
     1981 cctattgtga aggtcaataa tgcgcacact gcgacgcttg ggcgttccgg cgtgcgtcct
     2041 ttccggctct atgaccttcg ccacacatgg gcgacgcggg ctgcaatggc aaatgttgac
     2101 cttgttacgc tagccgcgat gcttggacac tcccgcgtcc aaatggtcat gcgctacgcg
     2161 cacccgaccg aagagcatca atttaacgcg atgaagaaag ttcaaacctt cgttgcgggc
     2221 ggactcaaat aattgtagta gtttagtttg aaatctgagc taagaagcat cacttggcgg
     2281 cagtgttttt agttagtaag cgcgtcacca tcgccgatag tcgcccaatg tcttgcggct
     2341 tcctcaagaa agcattgacg cctgcgcgta acgcgtcctg ctcaacgtcg cttgctgata
     2401 acatgatgat cggcgtgcgt cgccgatgtg gcagttggcg cgcacggcgc gcaagctcta
     2461 agccgtcctt gccgggcaac tgattatcga ggatgagtag gtgatagcgt gctttgcttt
     2521 caatcttgcg tatggcctct gtcccatcag cgcagagttc gaccgtccag cccagttcct
     2581 ctaaagtttc tttcaccgcg tcagctacta cctgataatc ttcgacgcag agaatattga
     2641 tagtgcgcgc tttattgcgc gggcgaccgc gacgactatt ccggttgatg atgctctgtt
     2701 tgcgcggttc aggcggcgtg cgcaagtagg cgaggtcttt atgcctgccc tgttcggcgt
     2761 ctaacatgta cgtaagcgtg gcatgatgac gcaaaccgag cagccgtgcg gctcgcgtaa
     2821 cgctcccacc tccgtctatc aaagcccgcc gcacatatct agcctcgtag gtacgcaccc
     2881 gctcgttgag atcgaagttc gcccaaaaat ctacgacgct gcggactcgt ctttgtgata
     2941 gggacacgcg acgatcggac acaatacgta tagcgcaggc gcgcagacgc gaaatcgttt
     3001 cagcatcctg cgtctctttg agcaaattat gtgcgcgctc gtatgcctct aatagttcat
     3061 gctcgttgag atgatcggcg tgttcctcca ttaacgacag caaagcgcgt ccggcatctt
     3121 caaccgctcc ggcttcctct gcaaggtcag cggcgcggcg caacgtgttc agcgattcga
     3181 tgaagttgcc gcgcttagag aggatgcggc cttgtgtggt gagggcttca gccaacaagc
     3241 cctgctcgcc gcctttggag agcacccgca cggcctccct gattgcgctt tcggcggcgc
     3301 gtagtctgtc ctgtgccagc aagacgcggg cgcgcgtctc atccacctgt gcaacgctgc
     3361 cctgatcact taccgtagcg aataagtggc gcgcacgatt taaatgttga tgcgcttcct
     3421 cgtatcgccc gacagtgtga agcaggaagc ctagattgtt ttcagcgcgg gcgaaataac
     3481 ttgtatgatc tgcccgctcg aagtgaacag atgctgctgt atattcgatg atagcgcgat
     3541 ctatgtaatc cgggcggcgt tcagctttac ttaagcacac aagtagacag gctagatcgt
     3601 tatgaaactt tcccttgagg aaatcattcg tgctttcttc aaagaatcgc gcctcatcta
     3661 ctaatatgcg caatgaatcg ttgtagcggc cactcagtga ttcaacgatt gcaagacgca
     3721 gtaatatttt agcccgcaac tctttctcac tgccgtcacc aagttctctc agtgcttctt
     3781 gataaactac ccgcgcatca tcgtatgctc cagcgcgacg gtaacagaaa ccaagttcgc
     3841 tccgcgcggc gaccactttg gttgtctctc ctaatgtttg aaagcgcgtt atactttcac
     3901 tgattaagtc tttggccttt tcttgcactc ccttaatctg ccgaccgctg ccgagccaac
     3961 cagagagcga accgacacgc aaaagaacct ctgcggcggt caactctgat aatccatcaa
     4021 gtgtaggacg ttcgccgacc ccctgccaca attcgccgag agcgtcccgc gccgactcgt
     4081 actcgcccct gtgctcgaag tcagccgcta ttttgcattg aagttgtgcg cgttcgttgc
     4141 ggctcaacgc acagttttcc aactgcctta ataaggtttc agctaaagtc atgtgaggac
     4201 tcgctttctt tgtgcttgct gagaaatttc aggcaggctt aagccccgtc ctatcaacag
     4261 aatagggcgc aaattaaaac cagtcgggtc tggtttcacc aactacctca gaggaaaaag
     4321 aagagatgaa aagctaccga aatgctattg ccgcgctggt cttggcattt gtgttctcca
     4381 cgtctgcctt cgccggtgac ggcataatgt ggactgacaa gactcctcca ccgccgccgc
     4441 cacaagcaga cggcataatg tggacggacg tagcctcccc cacatcggaa ggggatgccc
     4501 tgacggagat tgcgctcacc ttgctgcaaa ctctgatccc gctgctctaa ttcggcggaa
     4561 cagttcaagg taattgtatg ctgccgtgag gctcacaggc ggcgcggtct tcgtgtgccc
     4621 ggcgcgaata tacaacaatt acgatactct gcgagaaaat tctgagcgat gtaactacaa
     4681 gattaagtgt aaaggttggt gaggcttaaa tattgggaga gtgcaggagt gagatcacat
     4741 cgggctatat gtccagatcg ggagcgcgta ataaagttcc cacaaaagtc cccacagcac
     4801 gttttaatag tcgctgtggg gactttattt gtgactgtaa attgtagggc aaaaagaatt
     4861 attgaaggcg gcgatcggaa tcgaaccgat gaataaaggt tttgcagacc tctgccttac
     4921 cacttggcta cgccgccgga ggggaaggga aagtcgaaag gcgaaggcga aagttgagag
     4981 aggctttctc ctttcgctct tcgcctttcg acttgaactt tggagcggga gacgagattt
     5041 gaactcgcga cttcgacctt ggcaaggtcg cactctacca ctgagttact cccgctcagc
     5101 gaaaagagat tttaccgatt cgcccccgct tgtcaagcga gccttcgagg gaatttgaca
     5161 aggccgctcc ggcgcggtta atttcctcac gtcagcacct gtacaaactc cttgtaaatt
     5221 tagtttcgcc accactcagc cgaagttcaa aagaggcacg cgcgtccgcc agcgccagtg
     5281 cccgagaaag gtcgcccgct catgtcaaac acagattctt tgtcgccgtc gctcatgcct
     5341 tcaacgccgt cacacatgcg ttcgatgcgg tcgtccatgc gttcgacgct gtcgctctgc
     5401 gcgttgttgc tgttgcttgc ctccgtcgtc gccgcgcagg gctatcgtaa gccgccgcaa
     5461 gcggtgctcg acgtgctcaa ctcgcccgtc acgccttcga tctcgatcag tccggcgcgc
     5521 gaccaactgt tgctcgccac gggcgtccgc tatccgccca tcaacgatct ggcgcagccg
     5581 atgctgcgtc tcgccggata ccgcatcaac ccgaacacca acgggcggca tctcgcgcct
     5641 tatttcgtcg cgctctcttt gaagcgcatc gcggacggcg cggagacgaa gatcactctg
     5701 cctcccggcg caaaggccgg ctttccgcag tggagtccgg acgggcggca tttcgctttc
     5761 accaacacca tcgcgaacgg gatcgaacta tgggtgggcg atgctgcttc aggacgcgtg
     5821 acgaagatca aaaacgtact cgtcaacgcg gcctacggcg acccgctcgt gtggatgccc
     5881 gacaatcgca cgctgctcgc gcaactcgtg cccgccgcgc gcgccagact cgcgcctgcg
     5941 cccgatgtcc cgcgcgagcc gaacgtgcag gaaagctcag gccgcgccgg tccggtgcgc
     6001 acctatcaag acctgctcaa gacgccttac gacgaagagt tgttcgacta ctacgcgacg
     6061 gcgcaactcg cgctcataga cgtagcgagc gggcgcgtga cgaacatcaa ccagcccgcc
     6121 atctatgaga acgccgcgcc cgcgcccgac ggccagcacc tcctcgtcac gcgcctcaaa
     6181 cgcccttact cgtacctcca tccggccaac gattttccga aagatgtcga ggtgtgggac
     6241 acgcgcggca agttgattca caagctggcg agccagcctt tgcaggatca ggtgccgatt
     6301 gaaggcgtgc cgaccggacc ccgcgcgcac agttggcgtc cgaccgaacc ggcgaccatc
     6361 gtgtgggctg aagctctgga cgaaggcgac ccgaagaaga aggtcacgca tcgcgaccgc
     6421 ctgttgatat cgaaagctcc tttcacgggc gcgcctacgg agttgatgaa actcgaagac
     6481 cgctacgccg gaatccaatg gttggagcgc ggcggactcg ccttcgcgag cgactacaac
     6541 cgcgacacgc gccgcgtccg cacgcaactc gtcagcgtgg ataacgcgag cgtcgcgccg
     6601 cgcgtcgtct ggaaccgcaa cgtgcaggat cgctacaacg atcccggctc gcccatcacg
     6661 cgcatcctgc ccacaggcca ccgcgtgatc atctacaaca acgatcagat ttatctctcc
     6721 ggcgcgggcg catcgaacgc gggcgaccgt ccgttcctcg accgtttcaa tctcacgacg
     6781 ctcaagggcg aacgcatctt ccgcagcgac gagaagagtt atgaagcgtt cgtcgcgctc
     6841 ttgaaagacg acgcctcaca gttcatcacg cgccgcgaga cgccgattga gccgggaaac
     6901 tttttcatac gcacgctcgg cggcgcagcc gggacggcag cgacgggcga agcggccatg
     6961 acttcaacct cgaagccgct cacgcagttt ccagacccga cgccgcaact gcgcgacatc
     7021 aagaaacaac tcgtcaccta caaacgcgca gacggcgtcc aactctcttt cacgctctat
     7081 cttccgcccg gctacaagga aggcacgcgc ctccctacgg tcgtctgggc ttatccactg
     7141 gagttcaacg acgcggccac cgccggtcaa gtctccggct cgacgcaacg cttcacgacc
     7201 atcggcggcc cctcgcatct cttcttcctg ctgcaaggct acgccgttct ggataacacg
     7261 acgatgcccg tcgtcggcga tcccgaaacg gtcaacaaca cttatgtcga acagatcaac
     7321 gagagcgcga aggcggcgat tcagaaagcc gccgacatgg gcgtcaccga cccggaacgc
     7381 gtcggcgtcg gcggccactc ttacggcgcg ttcatgacgg cgaacctgct tgcgcacagc
     7441 gacctgttcc gcgcgggcat cgccagaagc ggcgcgtaca accgcacgct cacgcctttc
     7501 ggcttccaga gcgagcggcg cattttgtgg gaagcgccgg acatgtacat caaagtctcg
     7561 ccgtttatga acgcgcacaa gatcaaggag ccagtcttgt tgattcacgg agaggccgac
     7621 aacaacacgg gcacgttccc gatccagtcc gagcgcatgt atcaggccat acgcggcacg
     7681 ggcggaaccg tccgcctcgt catgctcccg ctcgaatcgc acggctacag cgcgcgcgag
     7741 acgaccgagc acgtgctctt tgaaatgctc aactggttcg acaagtacgt gaagaacgcg
     7801 ccgccgcgca cgcaggagca gaaagcctct gcctccacga agtaaaccgc gcttgtgcgc
     7861 tgcacgcgaa aaagcccgac cggattcaac gcccggtcgg gcttttctct gcggtgcacc
     7921 ccggttcaat ctcagccgtc tgatcaattt cggacgcggc tcagggcgag ttcgcgtctg
     7981 accagtgacg tttgcgaaat ttggcttcgg tccagaagtt gcgcaggagt tcgaggcgcg
     8041 ggttcggcgc gggcttttcg acggttgcgt gcgggaggat gacgctgacg acatcgtagc
     8101 gataaggaat gtttgccaga ttgaaagtgc ggcggtaggc gcgtgcggcg cgtgtg
//
LOCUS       DASWNS010000129         8382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_498991, whole genome shotgun sequence.
ACCESSION   DASWNS010000129 DASWNS010000000
VERSION     DASWNS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498991"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VGB76_04310"
     CDS             <1..565
                     /locus_tag="VGB76_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098159.1"
                     /translation="WFMTRQGESQKNQIAAGNNGSEAVESKQPEKAVVTDDKTGDKVS
                     DNNAEINNTVAPKQREDSAFVVKAATHDERRRAPHLLMAKRQKSQPRRGVENAPAPRE
                     LIATNSEPGWVVGRMNLRRSKNAPPPAPGLREFIAAHGMTDWQVEQPLTPQQRLAAEQ
                     LMLALRVASAKFNYAQREMQEIGRAGK"
     gene            611..1231
                     /locus_tag="VGB76_04315"
     CDS             611..1231
                     /locus_tag="VGB76_04315"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HEY0098160.1"
                     /translation="MRTKVNSWLKCVSLALALFASGAGAAAKAQQPQYAAKLQLDNEE
                     TLSQRASEFIDVNLDETLLRIIPKRIKTKTDDGKDIDIGAIIGGLRGVYVRSYGFNNE
                     GEYTEANLARVRTQLRSPGWARLVNIVKRKADENTHVEVYMMTSTGSIDGIAILALEP
                     KRLTLVNIVGNIDLEKLSQLEGQFGIPEIGIGSGGDDKEHKPAKKP"
     gene            1247..1792
                     /locus_tag="VGB76_04320"
     CDS             1247..1792
                     /locus_tag="VGB76_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098161.1"
                     /translation="MRRTFFSLLLIINASLVAVPVTATTTTKETPEYQAVVKLIESHY
                     QVKHRGIPFVANLGVKTAKVVSKDARRVLRFGSFKLAIFEDQDFSGRDATGDIRTRLR
                     RTLEPHWHPLVAARLEDEGQTHTFTKADGDKFKVLIVVIGQRDATVLQVNLNPEEFAK
                     LLLNPEQQSRELTNEATSVEQ"
     gene            1915..3456
                     /locus_tag="VGB76_04325"
     CDS             1915..3456
                     /locus_tag="VGB76_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0098162.1"
                     /translation="MRSQNFLSRGLRNGVSLALSLLLVCGGASFTVAQEQSAAVATAQ
                     DEVVKVRTRVVFLDALVTDKKTKTFASGLTPENFEVLADGKPRPVSYFTREGDKNRRP
                     LALAVVFDLERLGAGRYMRRTEILEAMAAELSKLPPEDEVAVIVLDPNGVEDRREWLT
                     PFTRNRAQVASAMAIVPTLVGEGAGGDGGSDEGAPAQEAAERRAPQAGANGATPAGQA
                     AESTEGAKKESGQPAKEKEKEKREKGITISIGTDKGVKVESSNAEKDKSVDEEDEEVE
                     VDYIKDRKGNTVKRIVKADGTMIIERRNKDGSTTSDYQEPFDLAAAALEITKRVAAER
                     PNSQPALVYITDGIAPVFYTQRDYVESKLLKSNVIFSALVTDMKTGFKFAMPLLKPLG
                     NLAGISISGSAQHLAKATGGESVRVRRPADYANGLSLIVGNLNARYSLGFALAEDEMD
                     DGQMHPLEVRVRAKDAKGKERKLEVKARRGYYMPTDATKKKSAEAAKKSESGDDKTQS
                     ERVKN"
     gene            3565..4674
                     /locus_tag="VGB76_04330"
     CDS             3565..4674
                     /locus_tag="VGB76_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0098163.1"
                     /translation="MREAVRIIFAVACCMLLHAPARAQQTAGALAANGQTPAGSNATG
                     NSGDAVTHVVSKWKFKTGGSVYSTPLITNGVAYFGSYDYYFYAVESATGRELWKYKTG
                     GRILGSPALSGGQVYFGSQDTRLYALDAATGKERWKFKAEYAVNSTPVVSGNIVYCAG
                     TVGLLYAIDAQTGRELWRFSAEAGAPTSPLVIDSMLYLGSGRGLHAVDAATGQKKWTA
                     RTSRPVVSAPVSGDGNETIYFTSLDRQLHAVDAATGERKWTFQTEGGLPFSPALAGGL
                     LYFGGIDGNLYAVDAATGKERWKFATAHGITSAPTVANALVHFGSLKGLYTLDAATGQ
                     LRWSYTTERIVTSTPAVSGNLIYFGGWDGNLYAVQ"
     gene            complement(4744..5070)
                     /locus_tag="VGB76_04335"
     CDS             complement(4744..5070)
                     /locus_tag="VGB76_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098164.1"
                     /translation="MNRIWMVAAVPFVLMSAMLTGFLVRPSWADTASPLTCMLVLAGA
                     VLTGLYVLYAVFAGWWRGSHPLRRGDILAATALAALDVLIPTTLGLLLFLLLRSMENS
                     RGLFMF"
     gene            5405..6295
                     /locus_tag="VGB76_04340"
     CDS             5405..6295
                     /locus_tag="VGB76_04340"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY0098165.1"
                     /translation="MEKLTIRRVGVLVKPQHPEAVKSICRLLEWLTPRGIKLIMGPRP
                     GNEREAIERETGCAVETLEHEELAASVDMIVVLGGDGTMIATARLLDNRDVPVVGINY
                     GSLGYLTEVRVEEMTDALAAILEGHYHLDRRVMLAAELYRGEEKLLRNRVLNDVVISK
                     SALARIIEIETWFDGQLVNCFRADGLIISTPTGSTAYNLSAGGPVVYPSMNAVVITPI
                     CPHTLSNRPLVVPDDVEIELILKTPDEEVALTLDGQVGYRLQVGDRVLISKSRTTFNL
                     MQPLNRNYFEVLRGKLKWGR"
     gene            6448..7968
                     /locus_tag="VGB76_04345"
     CDS             6448..7968
                     /locus_tag="VGB76_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEY0098166.1"
                     /translation="MADDNRDRDVTPPEVRSDAYERETTTDLKDATPDRPKGIALHTR
                     ILIGLAVGVLLGVLSFSLFGQPHPTLPGATVPHPRVEWVIANITEPVGTLFLRLLLMV
                     VVPLVFSSLVVGVAGIGDIRKLGRVGAKSFAYVLVISAISVVIGLTLANTIRPGTRLD
                     AATRSGLEARYGTDAEKRVADAKKTATTDSPLMQVVKTIVPENPVAAVAGIIRGEEKQ
                     TDTPNMLHLMFFAVILGIAATLISTEVTAPLLRGLQALFEISAKIIDIIMKIAPFAVA
                     CLLFNNTARFGLELLTALGWFMLTVLGGLALHMFGVYSLSIYFLSRISPLEFFRRIKT
                     VMLTAFSTSSSNATLPTALRVSEENLGVPREINSFVLTVGATANQNGTALYEGVTVLF
                     LAQLAGIDLTLGQQLGIAYFAILGGIGTAGVPSGSIPFIIVMLVTYGINPALIAIILG
                     VDRILDMCRTTLNVAGDITAATYVARSEGYELLKPQVPELERRGVVAGGPSSHDVI"
BASE COUNT         1789 a         2584 c         2567 g         1442 t
ORIGIN      
        1 atggttcatg actcgtcagg gcgagtcgca gaagaatcaa atcgcagcgg gtaacaacgg
       61 aagcgaggcg gttgagagca agcaaccgga aaaagcagtc gtcactgatg ataagaccgg
      121 cgacaaagtt tcggataaca acgctgagat caacaacaca gtcgcgccga agcagcgaga
      181 agatagcgcg tttgtagtta aagcggcgac tcacgacgaa cggcgacgcg cgccgcacct
      241 tctgatggcg aaacgtcaga agtcgcagcc gcgcagaggt gtggagaacg cgcccgcgcc
      301 gcgagagttg atcgctacca acagcgagcc gggatgggtc gtaggacgaa tgaatctacg
      361 acggagcaag aacgcacccc cgcccgcgcc cgggttgcga gagtttatcg ctgcgcacgg
      421 gatgacggat tggcaggtcg agcaaccttt aacaccgcaa cagcggttgg cggctgaaca
      481 gttgatgctt gcgctccggg tggcgagcgc gaagttcaat tacgcgcagc gcgagatgca
      541 ggagatcggg cgcgccggaa aatagcgcgc aggtaaagat tcagaatgca ggcggaagaa
      601 tggagtaatt atgagaacga aagttaattc atggctgaaa tgtgtatccc tcgctctcgc
      661 cctgttcgcg tcgggagccg gggcggcagc aaaggcgcag caaccgcagt acgctgcgaa
      721 gctgcaactt gacaacgaag agacgctctc gcaacgtgcg agcgaattca tcgacgtgaa
      781 cctcgacgag acgctgctgc gcatcatccc gaagaggatc aagacgaaga cggacgatgg
      841 caaggacata gacatcggcg cgatcatcgg cggcctgaga ggcgtctacg tgcgcagcta
      901 cggcttcaac aacgagggcg aatacacgga agccaacctg gcgcgagtgc gcacgcagtt
      961 gcgctcgccc ggctgggcgc ggctcgtcaa catcgtcaag cggaaggccg acgagaacac
     1021 gcatgtcgag gtttatatga tgacctccac cggcagcatc gacggcatcg ccatcctcgc
     1081 gctcgaaccc aaacggctca cgctcgtcaa catcgtcggc aacatcgacc tcgaaaagtt
     1141 gagccaactc gaaggccagt tcggcatccc cgaaatcggc atcggcagcg gcggcgacga
     1201 caaagagcac aagcccgcga agaagcctta ggaaagggga cagttgatgc gccggacatt
     1261 cttctcactg ctactcatca tcaacgcgtc gctcgtcgcc gtgccggtca cggcgacaac
     1321 gacgacaaaa gagacgcccg aatatcaagc ggtcgtcaaa ctcatcgagt cgcactatca
     1381 agtcaagcat cgcggcatcc ccttcgtcgc caacctcggc gtgaagaccg cgaaggtcgt
     1441 gtcgaaggac gcgcgacgag tgctgcgctt cggcagtttc aaactggcga tctttgaaga
     1501 tcaggatttt tcgggacgcg acgcgacggg cgacatccgc acgcgcctgc gccggacgct
     1561 cgaaccgcac tggcatccgc tcgtcgccgc gcggctagag gatgaagggc agacacacac
     1621 tttcacgaag gccgacggcg acaagttcaa agtgctcatc gtcgtcatcg gccagcgcga
     1681 tgcgaccgtc ctgcaagtca atctcaaccc ggaagagttt gccaaactcc tgctcaaccc
     1741 cgaacagcag agccgcgaac tgacgaacga agcgacgagt gtggaacagt agaacagcag
     1801 ggatgaagga tgagggatga gggatgaata agaaaaatac tcaagcaagt taatcattcc
     1861 tgttcatccc tcgtccctca tccttcaccc ctttctttaa ccggagtatt gaaaatgaga
     1921 agccagaact ttttatcgcg cggcctgaga aacggagtct ccctcgcgct ctcgctgttg
     1981 cttgtgtgcg gcggcgcgtc gttcacggtg gcgcaggaac agtctgccgc cgttgcgacg
     2041 gcgcaggatg aggtcgtcaa agtcagaacg cgcgtggtct tcctcgatgc gctcgtgacg
     2101 gataagaaga cgaagacgtt tgcgtcgggg ctgacgccgg aaaacttcga agtgctcgcc
     2161 gacggcaagc cgcgccccgt ctcctacttc acgcgcgagg gcgacaagaa tcgtcgcccg
     2221 ctcgcgctcg ccgtcgtctt cgatctggaa cgcctcggcg cgggtcgcta catgcgccgc
     2281 acggagattc tcgaagcgat ggcggcggaa ctctccaaac ttccgcccga agatgaagtg
     2341 gccgtgatcg tgctcgaccc gaacggcgtg gaagaccgcc gcgaatggtt gacgcctttc
     2401 acgcgcaacc gcgcgcaggt cgcttccgcg atggcaatcg tcccgacgct cgtgggcgaa
     2461 ggcgcgggcg gcgacggcgg cagcgacgag ggtgcgcccg cgcaggaagc agccgaacgg
     2521 cgcgcgccgc aggccggtgc gaacggcgcg acgcccgccg ggcaggcggc ggagagcacg
     2581 gagggcgcga agaaagagag cgggcagcca gcgaaggaga aagagaaaga gaagcgcgag
     2641 aagggaatca cgatctcgat tgggacggac aaaggcgtga aggtcgaatc gtcgaacgcg
     2701 gagaaagaca agtccgttga cgaagaggac gaagaagtag aagtcgatta catcaaggac
     2761 aggaagggca acacggtcaa gcgcatcgtc aaagcggacg gcacgatgat catcgaacgc
     2821 aggaacaaag acggctcgac gacgagcgat taccaagagc ctttcgatct cgcggcggca
     2881 gctttggaga tcacgaagcg cgtcgcggcg gagcgtccca actcgcagcc cgcgctcgtc
     2941 tacatcacgg acggcatcgc gcccgtcttc tacacgcagc gcgattacgt cgagtcgaaa
     3001 cttttgaagt cgaacgtcat cttcagcgcg ctcgtgacgg acatgaagac gggcttcaag
     3061 tttgcgatgc cgcttttgaa accgctcggc aacctcgcgg gcatcagcat ttcggggagc
     3121 gcgcagcatc tcgccaaagc gacgggcggc gagtccgtac gcgtgcgccg acccgccgac
     3181 tatgccaacg gcctgagtct catcgtcggc aatctcaacg cgcgctacag cctcggcttc
     3241 gcgctcgccg aagacgagat ggacgacggc cagatgcacc cgctcgaagt gcgcgtccgc
     3301 gcgaaggatg ccaaaggcaa ggaacgcaag ctcgaagtga aagcgcggcg cggctattac
     3361 atgccgaccg acgccacaaa gaagaagtcc gcagaggccg cgaagaagag cgagagcggc
     3421 gacgacaaga cgcagagtga acgcgtgaag aactgagagc gagccgtcaa gacacggaga
     3481 cgcaaagttg aatcaacaag tcttctttcc gtcttcgcgt ctcgatggca gaccgtttcg
     3541 gacgctttga accagaggct caacatgaga gaggcagtca ggataatctt tgccgtagca
     3601 tgctgcatgc tgctgcacgc tcccgctcgc gcacagcaga cggcgggagc gttagccgcg
     3661 aacgggcaaa ctccggcggg tagcaacgct acgggcaaca gcggcgatgc ggttacgcac
     3721 gtcgtctcga agtggaaatt caagacgggc gggagcgtct attcgacgcc cctgatcacg
     3781 aatggcgtcg cctacttcgg cagctacgat tactactttt acgccgtcga gagcgcaacc
     3841 gggcgcgagc tttggaagta caagacgggc gggcgcatcc tcggttcgcc cgcgctctcc
     3901 ggcggccaag tctatttcgg cagccaggac acgcgcctct acgcgctcga tgcggcgacg
     3961 ggcaaggagc gttggaagtt caaagccgag tacgcggtga actcgactcc ggttgtcagc
     4021 ggcaacatcg tctattgcgc ggggacggtc ggcctgctct acgcgattga cgcgcagacg
     4081 gggcgggagt tgtggcgttt cagcgcggag gcgggcgcgc ccacttcgcc gctcgtcatc
     4141 gactcgatgc tctacctggg gagcgggcgc ggcctgcacg cggttgacgc cgccacgggg
     4201 cagaagaagt ggacggcgcg aacctctcgc cccgtcgtct ccgcgcccgt ctccggcgac
     4261 ggaaacgaga cgatctactt cacgtcgctc gaccggcaac tccacgccgt tgacgccgcg
     4321 accggagagc ggaagtggac tttccagacg gaaggcggcc tgcccttctc gcccgcgctc
     4381 gcaggcggtc tgctctattt cggcggcatt gacggcaacc tctacgcggt tgacgccgcg
     4441 acgggcaagg agcgttggaa gtttgccacg gcgcacggca tcacttccgc gccgaccgtc
     4501 gccaacgcgc tcgtacactt cggcagcctc aaaggtctgt acacgctcga cgccgcgacc
     4561 gggcaactga gatggtctta cacgaccgaa cgcatcgtca cctcgacgcc cgccgtctcc
     4621 ggcaacctca tctacttcgg cggctgggac ggcaacctct acgcggtaca gtgaaagggc
     4681 aggagtcaga attcagaatt caggagtcag agttaacaac ctctcagctt ctcttctgac
     4741 tccctaaaac ataaatagcc cgcgactgtt ttccatgctg cgcaggagca ggaagagcag
     4801 cagtccgagc gtcgtcggga tgagcacgtc gagcgcggcc agcgccgtcg ccgcgagaat
     4861 gtcgccgcgc cggagcgggt gcgagccgcg ccaccagccc gcgaagactg cgtacaacac
     4921 gtacagcccc gtcaagaccg cgcccgcgag cactaacatg cacgttaaag gcgaagccgt
     4981 atcagcccac gacggcctca ccaagaagcc ggttaacatc gccgacatca acacgaacgg
     5041 caccgccgcc accatccata ttctgttcat cgctttgagt ctcactctct ttcgcacgct
     5101 tgaatttcgt cgtcgcctgc cgccgtcagg ttgctcatag cttagagggc gggcgcgccc
     5161 gctcgcccgc ctgcgtatga cacttcctgc tttgtcacac gggctggcgc ggcggttgaa
     5221 aataactgcg cgttgacact cggaaagacg cgcttgtatc atcccacgca gttttccgcg
     5281 ctctgaaagt tagcctgact ttgccagaag aatgttgccg gatagtcggt tgcgtgcgcg
     5341 cttgcagcgg cgtcggtcgc agcgtgcggg gcatgcgcag tctctccgcc gtcgtgtgtg
     5401 gctgatggaa aaacttacta ttcggcgcgt cggcgtcctc gtcaaaccgc agcacccgga
     5461 ggcggtcaag agcatctgtc gcctgctcga atggctcacg ccgcgcggca tcaagctcat
     5521 catgggaccg cgccccggca acgagcgcga ggccatcgaa cgagagacgg gatgcgccgt
     5581 cgaaactttg gagcacgagg aactcgccgc gagcgtggac atgatcgtcg tgctcggcgg
     5641 cgacggcacg atgatcgcca ccgcgcgcct gctcgataac cgcgacgtgc ccgtcgtcgg
     5701 catcaactac ggcagcctcg gttatctcac cgaagtgcgc gtcgaagaga tgacggacgc
     5761 gctcgccgcc atcctcgaag gccactatca tctcgaccgc cgcgtgatgc tcgccgccga
     5821 actgtatcgc ggcgaagaga aacttttgcg caaccgcgtc ttgaacgatg tcgtgatcag
     5881 caagtcggcg ctcgcgcgca tcatcgagat cgagacgtgg ttcgacgggc aactcgtcaa
     5941 ctgttttcgc gccgacggcc tcatcatctc cacccccacg ggttcgacgg cctacaacct
     6001 ctcggcgggc ggccccgtcg tctatccttc gatgaacgcc gtcgtcatca cgcccatctg
     6061 cccccacacg ctctcgaatc gccccctcgt ggtgccggac gacgtcgaaa tcgaactcat
     6121 cttaaagacg cccgacgaag aagtggcctt gacgctcgac gggcaggtcg gctaccgcct
     6181 gcaagtcggc gaccgcgtgc tcatctcgaa gagccgcacg acctttaacc tgatgcagcc
     6241 tctcaaccgc aactactttg aagtgctgcg cggaaaactg aaatggggaa gatgagaagg
     6301 cagtgacaag tgacgagtga taagtgacga gagaagaaag tgagtcgtca atcgtcaata
     6361 gataaaggcg gtttcccttc tattcacgat tcacggcttt tgtttcaact tgtcactcgt
     6421 cacggttctt aatcttgagg agaaaaaatg gctgacgaca atagggatcg agatgtgacg
     6481 ccgccggaag ttcgctccga tgcttacgag cgcgagacga cgaccgattt gaaagatgcg
     6541 acgccggatc gcccaaaggg catcgcgctg cacacgcgca tcctgatcgg gctggcggtc
     6601 ggcgtgcttc tgggcgtgct gagtttcagc ctgttcggcc agccgcaccc gaccctcccc
     6661 ggcgcgaccg tcccgcatcc gagggtcgag tgggtgatcg caaatatcac cgaacccgtc
     6721 gggacgctct tcctgcgcct gctgttgatg gtggtcgtcc cgctcgtctt ctcgtcgctc
     6781 gtggtcggcg tggcgggcat cggcgacata cgcaaactcg ggcgcgtcgg cgccaagtct
     6841 ttcgcctacg tcctcgtgat ctcggccatc tccgtcgtga tcggcctgac gctggcaaac
     6901 acgattcgtc ccggcacgcg cctcgacgcg gcgactcgct ccggcctcga agcgcgctac
     6961 ggcacagacg ccgagaagcg cgtggccgat gcgaagaaga ccgccacgac cgactcgcct
     7021 ctgatgcagg tcgtcaagac catcgtgccg gagaaccctg tggcggcggt ggcgggcatc
     7081 atcaggggcg aagagaaaca gaccgacacg ccgaacatgc tccacctcat gttcttcgcc
     7141 gtcatcctcg gcatcgccgc gacgctcatc tcgaccgaag tgaccgcgcc gctcctgcga
     7201 ggcttgcagg cgctctttga aatctcggcg aagatcatcg acatcatcat gaagatcgcg
     7261 ccgttcgccg tcgcctgcct gctcttcaac aacacggcgc gcttcggcct cgaactgttg
     7321 acggctctcg gctggttcat gctcaccgtg ctcggcggcc tcgccctgca catgttcggc
     7381 gtctattcgc tctcgatcta tttcctttcg cgcatctcgc cgctcgaatt tttccgtcgc
     7441 atcaagaccg tcatgctcac ggccttctcc acctcttcct caaacgcgac gctcccgaca
     7501 gccctgcgcg tctcggaaga gaacctcggc gtgccgcgcg agatcaacag cttcgtgctc
     7561 acggtcggcg cgacggccaa ccagaacggg actgcgctct atgaaggcgt caccgtgctc
     7621 tttctcgccc aactcgcggg cattgatctg acgctcgggc agcaactcgg catcgcctac
     7681 ttcgccatcc tcggcggcat cggcacggcg ggcgtcccct ccggctccat ccctttcatc
     7741 atcgtcatgc tcgtgaccta cggcatcaac cccgcgctca tcgccattat cctcggcgtg
     7801 gatcgcatcc tcgacatgtg ccgcaccacc ttaaacgtcg cgggcgacat cacggcggcg
     7861 acctacgtgg cgcgctccga aggttacgag ttgttgaagc cgcaggtgcc cgaactggaa
     7921 cggcgcggcg tggtggcggg aggcccttcg agccatgatg tgatttgacg gacgccgccg
     7981 gagtgaaagc gtgcgccgga gatgtatgag aacgatgctg gacttgaaag cccgctcgcc
     8041 ggagtgtgag cgggctttga tgtttttggc ggagaaaggt gctcgcggcg cggtgttagt
     8101 tgcgcgcaac gggttgggaa cgtgagatgc aagaagatat tccttaactt ctcgaacagg
     8161 ctttgcaaca ttgtcgcgcg aagatggcgg ctcattacgt tagcgtctga gggagttgtc
     8221 cgttcgtgcg ccggaagcgc gcgccgggag gcagggcggg aagttagcgc gcccgtgtct
     8281 cgaacgttcg gcagaggaag aaaatatgtt tcccattggc gatgacaatt ccgaccggca
     8341 ctcgacgccg ttcgtcaact acgtcctgat cgcggccaac at
//
LOCUS       DASWNS010000130         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_83065, whole genome shotgun sequence.
ACCESSION   DASWNS010000130 DASWNS010000000
VERSION     DASWNS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83065"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGB76_04350"
     CDS             <1..203
                     /locus_tag="VGB76_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098167.1"
                     /translation="PDGTVFKNVQFNGKGYLMGGCMGCHGNAQFGGVGFSFIFGGGPV
                     PAPETDDPPTSLDKFVRLFAGH"
     gene            complement(307..2220)
                     /locus_tag="VGB76_04355"
     CDS             complement(307..2220)
                     /locus_tag="VGB76_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEY0098168.1"
                     /translation="MTTSIQSSETLPRAHSVAIVGLGPKGLYCLERLLAEFNARPLRQ
                     PLHLHLFNRSAHFGASPIYDPAQPEYILVNISVGEIDLWAAEDPPVVAGRGADFLNWY
                     QQTFQPQTPLKGDEYLSRAVVGRYLTEGFARILRHLPGGVTVSCHVAEVSDVRPDGRV
                     YQLEFMHASGGAEEIQVEKVLLATGHSRLMPGIEESRYASFAARHVDRASFIPFVYPV
                     VERMRGIPAGASVAMQGVGLTFIDAVLELTEGRGGRFERSAGGVLAYRPCGEEPRVVF
                     PYSRTGLPMTPKAHDLPLFARPLTFFTHRALAELRRRAPGGRLDFERDLWPLFELEME
                     LHYYRVVFGDGDERRALEACGNDARAMRRVIEAYLRAHPEQQPFDYRPVLDPAGAHRF
                     ESAAEFDAFVGRYMEAEIARARAGHAGSGIKSAIDIWYEVRSVLGSFLQFGGLTPESH
                     RKLIEHHYPRLKRVAFGPPIINIEKLLALQRARLLDFSVAQSPRVLTDEASGRFELRC
                     EELPGAVAQVETLVDARYPATDISRDASPLYRNLQRRGMVRAFTNHLTSRDSFAYRPG
                     AIDMTEEARFVVDGEGSGNEDIAVIGIPTEGNLTGNLTVARDKYAGIWAAKVIEQLRS
                     RELAWSASLDETV"
     gene            2341..>2810
                     /locus_tag="VGB76_04360"
     CDS             2341..>2810
                     /locus_tag="VGB76_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY0098169.1"
                     /translation="MEQDTDSPGLIYQPAVGGSSTFSALPQLAQSVAEEIDLAVLGIP
                     FDGLVTYRPGARFGPRAIRQSSVLCRGYSPQMEVQVYERLRAFDAGDVNVNLFDYEET
                     FRRIEARVADLQSRGAAVVSLGGDHSILLPILRATSRKYPDLTLIQFDAHTDTS"
BASE COUNT          538 a          905 c          840 g          527 t
ORIGIN      
        1 agcccgacgg cacggtcttc aagaacgtcc aatttaatgg gaagggctac ttgatgggcg
       61 gctgtatggg atgccacggt aacgcacaat tcggaggcgt cggcttcagt ttcattttcg
      121 gcggcgggcc ggtgcccgcg ccggagacgg atgatccgcc gacgagtctg gataagttcg
      181 tccggctgtt tgcgggacat taacccgtaa aagactccgg tgcagacgcc ggagcatgaa
      241 gatgctcgcc agcatgaaag aagtgctggc gagcatcttt ttagtactgc gccgctccga
      301 acggagctac acagtctcgt ctaacgaagc agaccacgcg agttcgcggc tacgcaactg
      361 ttcgatcact ttggccgccc agatgcccgc gtacttgtcc cgcgccaccg tcaggttgcc
      421 ggtgagattt ccttccgtcg ggattccgat cacggcgatg tcttcgttgc cgctgccctc
      481 tccatcgacg acgaagcgcg cctcctccgt catgtcaatc gcccccggac gataggcgaa
      541 actatcgcgg ctcgttaagt gattcgtaaa agcgcgcacc atgccgcgcc gttgcaggtt
      601 gcgatagagc ggcgacgcat cgcgcgagat gtcggtcgcg ggatagcgcg cgtcaaccag
      661 tgtctcgacc tgcgcaaccg cgccgggaag ttcctcgcaa cgaagttcaa agcgaccgct
      721 cgcttcgtcg gtcaacacgc gcggactctg cgccacggag aaatcgagca gccgtgcgcg
      781 ttgcagcgcg agcagttttt cgatgttgat gatcggaggt ccgaaggcca cgcgcttgag
      841 gcgcgggtaa tggtgctcga tcaatttccg gtgcgactcc ggcgtgaggc cgccgaactg
      901 taggaacgaa ccgagcacgc tcctcacttc gtaccagatg tctatggccg acttgatgcc
      961 gctcccggcg tgccccgcgc gtgcgcgtgc gatctccgct tccatgtatc gcccgacgaa
     1021 ggcgtcaaac tccgccgcac tctcgaaacg atgcgcgcct gccggatcga gcacgggacg
     1081 ataatcgaaa ggctgctgtt cgggatgcgc ccgcaggtag gcttcaatca cacggcgcat
     1141 cgcccgggca tcattcccgc acgcctctaa cgctctccgc tcgtcgccgt ctccgaacac
     1201 tacgcggtag tagtgcagtt ccatctcaag ttcgaagaga ggccataagt cgcgctcgaa
     1261 gtcgagccgt ccgccgggcg cgcggcgtct cagttcggcc agtgcgcggt gtgtgaaaaa
     1321 ggttagaggt cgcgcgaaga gcggcaggtc gtgcgccttg ggcgtcatgg gcaggccggt
     1381 gcggctgtag gggaagacga cgcgcggctc ctctccgcac ggcctgtaag cgagcacgcc
     1441 tccggcggat cgctcgaagc gaccgccgcg cccttccgtg agttcgagca cggcatctat
     1501 gaaagtcagc cccacgccct gcatcgccac cgacgcgccc gccggaattc cccgcatcct
     1561 ctccacgacc ggatagacga acgggatgaa cgacgcacga tcaacgtgcc gcgcggcgaa
     1621 cgacgcataa cgcgactctt caattcccgg catgagccgc gaatgccccg tggcgagcag
     1681 aaccttctcc acctgtatct cttccgcccc accgctcgcg tgcataaact caagttgata
     1741 gacgcgcccg tccggcctga catccgacac ctccgcgacg tgacatgaga cggtcacgcc
     1801 gccgggcagg tgcctgagga tgcgcgcaaa accctctgtg agataacgcc cgacgacggc
     1861 tctagataaa tactcatctc ccttcagagg cgtctgcggc tggaaagtct gctggtacca
     1921 gttgaggaag tccgcgcccc tgcccgccac cacgggcggg tcttccgccg cccataggtc
     1981 tatctctccg acgctgatgt tgacgaggat atattcgggc tgcgcggggt cgtagatggg
     2041 gctggctccg aaatgcgcgg agcgattgaa gaggtgcagg tggagcggct ggcgaagcgg
     2101 gcgcgcgttg aactcggcca gcaatctttc cagacagtac agacctttgg ggccgagtcc
     2161 gacgatggcg acagagtgag cgcgcggcag cgtttcgctc gattgaatgc tcgtagtcaa
     2221 ttcatcttcc tcgatgcatt ttattgacat caaagtagga aaacgccaga ctagccgcct
     2281 ctgtagtctc cggcgatccc gctctcgcta aaatctcaaa tcgaatggaa acgcgcgccc
     2341 ttggaacaag acacggactc tcccggctta atttaccagc ctgccgtcgg cggcagttcg
     2401 accttcagcg cactgccgca actggcgcag tccgtcgccg aagagatcga cctggccgtg
     2461 ctcggcattc ccttcgacgg tctggtcacg taccgccccg gcgcaagatt cggacctcgc
     2521 gccatacgtc aatcctccgt cctctgtcgc ggttatagcc cgcagatgga agtgcaggtc
     2581 tacgagcgtc tgcgcgcctt cgatgcgggc gacgtgaacg tcaacctctt cgattatgaa
     2641 gagacgtttc gccggatcga agcgcgcgtc gcagacttgc agagccgtgg cgcggcggtc
     2701 gtctctctcg gcggcgacca ttccatcctg ctgcccatcc tgcgcgcgac gagccggaaa
     2761 taccctgacc tcacgctcat tcagttcgac gcccacacgg acacctccga
//
LOCUS       DASWNS010000131         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_31542, whole genome shotgun sequence.
ACCESSION   DASWNS010000131 DASWNS010000000
VERSION     DASWNS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31542"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..212)
                     /locus_tag="VGB76_04365"
     CDS             complement(9..212)
                     /locus_tag="VGB76_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098170.1"
                     /translation="MAKIEARRIARRLILLGALVAGLVVQSASYNSQTAAACTENAPL
                     ICCSACEADPPPLPCRRGCSPSC"
     gene            487..1713
                     /locus_tag="VGB76_04370"
     CDS             487..1713
                     /locus_tag="VGB76_04370"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEY0098171.1"
                     /translation="MAVIYPFRALRPPFEKVEQVASVPYDVVNTEEARALAAGNPLSL
                     LHVTRPEIDLPEGTNIYSDEVYARAAENFQKLIETCPLETESEPSLYLYRLVMGEHEQ
                     VGIAACCAVDEYDEGLIRKHERTRPDKEDDRTRHMLELRAQTGVVFLTYRADRRIDAL
                     VAAIVAGDPLYDFTADDAIRHTVWRAPAPDALVRAFREVPLLYIADGHHRAASASRAR
                     ATLREQNPGHTGDEEYNRFLTVIFPSDQMQILPYNRIVRDLHGMSPEEFLAAVGNKFR
                     VEEGTAAVPPRGSWSMYLKQRWYSLTLLDGAATPSDDPTVALDVSVLQDHLLDPILGI
                     KDVRTDKRIDFVGGIRGTAELEKLVNEGRAQVAFSLSPTTIEDLLTVSDANGIMPPKS
                     TWFEPKLRDGLLIHQI"
     gene            1796..2374
                     /locus_tag="VGB76_04375"
     CDS             1796..2374
                     /locus_tag="VGB76_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0098172.1"
                     /translation="MNFTIETDRLLLRPLTLGDLPTLLSIRGKEQVSRYLGTSAVQTP
                     EFVEARLHVYLECYRLHGFGMAAAIEKTSGEFVGWGGLQPLEFGWTGERSAQAEQEVE
                     VGYGFDTPHWGKGYATELAAAWLRYGFEGAKLARIVAVASPENRGSWRVMEKLGMKYV
                     TTEPHYGTPCVLYAISREEFVPRGAYRLRSDS"
     gene            2395..3603
                     /locus_tag="VGB76_04380"
     CDS             2395..3603
                     /locus_tag="VGB76_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HEY0098173.1"
                     /translation="MKVLIADKFEQSGRDGLEAAGCDYSYQPDVKEESLVEAIRAYAP
                     DALVVRSTKVSEAMLDAGALKLVVRAGAGYNTIDVTAASRRGIYVSNCPGKNSIAVAE
                     LTFALILALDRRIADNVSTLRQGQWNKKEFSKARGLYGRTLGLVGTGQIGQEVIRRAL
                     AFGMRVVAWSKSLTQERAAELGIERASSPIDVARAADIVSVHVALKPETRGFIGADFF
                     GAMREGAYFINTARGEVVDQNALLAAMHTRGLRAGLDVFAAEPAGATGEFTDEIAREA
                     NLYGTHHIGASTDQAQEAIAAETVRIIRTFKETGKVPNVVNLAARTPATHLLVVRHRD
                     RPGVLAQVLDAIRAAHINVQEMENIVFEGAEAAVARINLEAAPPATTLEKLRTENADI
                     IELDLLELRS"
BASE COUNT          724 a         1105 c         1156 g          657 t
ORIGIN      
        1 catttcgttt agcaactcgg gctgcaaccg cgacgacacg ggagcggggg cgggtctgcc
       61 tcgcaggccg agcagcagat gaggggggcg ttttccgtgc aggccgccgc cgtctgcgag
      121 ttatagctcg ccgactggac aactaagccc gccacgagcg cgcccaaaag gataagcctt
      181 cgcgctattc ttcgcgcttc gatttttgcc atgatctttc tccttggtga aggttgtttg
      241 gttgtgagtc tgacgcgggc tgaggacacg cgccaacagg aaaagacatc gagtgaacgc
      301 gggaaatgta ttcccgtttt ccggtgcatg tcaatagcga gcggcgtggg ctcggcggag
      361 taaaactttc gcctgcgggc gcgtggaaag tgcgctcgcg gcttggcaaa ggggtgcggg
      421 tgcggatagt cttaggggtt agaaatccag cattttcatc ttctgtcgtg aggagaacga
      481 acgcccgtgg ctgtcattta tcctttccgc gctttgcgcc cgccgtttga gaaggtcgag
      541 caggtggcga gcgtgcctta tgacgtggtc aacaccgaag aggcacgcgc gctcgccgcg
      601 ggtaatccgc tcagtctcct gcacgtcacg cgcccggaga ttgatctgcc cgaaggcacg
      661 aacatttaca gcgacgaggt gtacgcacgc gccgcagaga attttcaaaa gctcatcgag
      721 acgtgtccgc tggagacgga aagcgagccg agcctctacc tgtatcgtct cgtgatgggc
      781 gagcacgaac aggtcggcat cgcggcctgc tgcgcggttg acgagtatga tgaaggcttg
      841 atccgcaagc acgagcgcac gcgccccgac aaggaagacg accgcacgcg ccacatgctc
      901 gaactgcgcg cacagacggg cgtggtcttt ctcacttatc gcgccgaccg tcgcatagat
      961 gcgctcgtcg ccgccatcgt cgcaggcgac ccgctctacg acttcaccgc cgacgacgcc
     1021 attcgtcaca ccgtctggcg tgcgcccgcg cctgacgcgc tcgtgcgcgc cttccgcgaa
     1081 gtgcctctgc tctacatcgc cgacgggcat caccgcgcgg cgagcgcaag ccgcgcccgc
     1141 gccaccctgc gcgagcagaa tccggggcac acgggcgacg aagagtacaa ccgttttctc
     1201 acggtcatct tcccgtccga ccagatgcag attcttccct acaatcgcat cgtcagagac
     1261 ttacacggca tgtcgcccga agaatttctc gcggcggtcg ggaataagtt tcgcgtcgag
     1321 gaagggacgg cggcggtgcc tccgcgcggg agttggagca tgtatctcaa acaacgctgg
     1381 tacagcctga cgctgctcga cggcgcagca acgccgagcg acgatccgac cgtcgcgctc
     1441 gacgtgagcg tgctgcaaga ccacctgctc gatcctattc tcggcatcaa agacgtgcgc
     1501 acggacaaac gcattgattt cgtcggcggc atacgcggca cagcggaact cgaaaagctc
     1561 gtcaacgaag ggcgcgcgca ggtcgccttc tcgctctcgc cgacgaccat cgaagacctg
     1621 ctcacagtct ccgacgccaa cggcatcatg ccgccgaaat cgacgtggtt cgagccgaaa
     1681 ctgcgcgatg gtttgttgat tcatcagatt taggaagaat gcgtgcgtgt ttgcccgaag
     1741 ccttgaccgg aacggttgaa gccggaattg ataagcgcaa cggcgcgtct atgctatgaa
     1801 cttcaccatt gagacggaca gactcctgtt gcgcccctta acgctcggag acttgcccac
     1861 gctcctctcg atacgtggta aagagcaagt ctcacgttat ctcggcacgt ccgcagtgca
     1921 gacgccggag ttcgtagaag cgcgcctgca cgtctatctc gaatgctacc gtctgcacgg
     1981 cttcggcatg gcggcggcca tcgaaaagac gagcggcgaa ttcgtcggtt ggggcgggct
     2041 tcagcctctg gagttcggct ggacgggaga gcgttcggcg caggcggagc aggaggtcga
     2101 ggtcggctat ggcttcgaca cgccgcactg gggcaagggc tacgccaccg aactcgccgc
     2161 cgcgtggctg cgttacggct tcgagggggc gaaactcgcg cgcatcgtgg cggttgcgtc
     2221 gccggaaaat agaggctctt ggcgggtgat ggaaaaactc ggcatgaaat atgtgacgac
     2281 cgagccgcac tacggaactc cgtgcgtgct ctacgcgatc tcgcgcgaag agtttgtgcc
     2341 gcgcggcgcg taccggttac ggtctgattc ttaacggacg agaggcggag gcgtatgaaa
     2401 gttctcatcg cagacaaatt cgaacaatcg gggcgcgacg ggctggaggc cgccgggtgc
     2461 gattattcct accagccgga cgtgaaagag gagtcgctcg tcgaagcgat cagggcttac
     2521 gcgccggacg cgctcgtcgt gcgttcgacg aaagtttcgg aagcgatgct ggacgcgggc
     2581 gcgttgaaac tggtcgtgcg cgcgggcgcg ggctacaaca cgattgacgt gacggcggct
     2641 tcgcgccggg gcatctacgt ctcgaattgt cccggcaaga attccatcgc cgtcgccgag
     2701 ttgaccttcg ccctgatcct cgcgctcgac cgtcgtatcg ccgataacgt ctccacgctc
     2761 cggcaagggc aatggaacaa gaaggagttt tccaaagcgc gcggcctcta cgggcgcacg
     2821 ctcggactcg tcggcacggg acagatcggg caggaggtga ttcgtcgcgc gctggccttc
     2881 gggatgcggg tcgtggcttg gagtaaaagt ctgacgcagg agcgcgcggc ggaactcggc
     2941 attgaacgcg cgtcgagtcc cattgatgtc gcacgcgcgg cggacatcgt gagcgtccac
     3001 gtcgctttga aacctgagac gcgcggcttt atcggtgcgg acttcttcgg cgcgatgcgc
     3061 gaaggcgcgt acttcatcaa cacggcgcgc ggcgaagtgg tagaccagaa cgcgctgctc
     3121 gcagcgatgc acacgcgtgg cttgcgcgcc ggactcgacg ttttcgccgc cgaaccggct
     3181 ggcgcgacgg gcgagttcac agacgagatc gcgcgtgaag ccaatctcta cgggacgcat
     3241 cacatcggag cttcgaccga tcaggcgcag gaagccatcg ccgccgaaac cgtgcgcatc
     3301 atccgcacgt tcaaggagac gggcaaagtg ccgaacgtcg tcaacctcgc cgcgcgcacg
     3361 cccgcgacgc accttctcgt cgtgcgccac cgcgaccgcc ccggcgtgct cgcgcaagta
     3421 ctggacgcca tccgcgccgc acacatcaac gtgcaggaga tggagaacat cgtcttcgaa
     3481 ggcgcggaag ccgccgtcgc gcgcatcaac ctcgaagccg cgccgcccgc cacgacgctc
     3541 gaaaagttac gcaccgaaaa cgcggacatc atcgaactag atttgcttga acttcggagt
     3601 taaaattatt tcaatacgcc agaacgggga ttgataacga tg
//
LOCUS       DASWNS010000132         6302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_160574, whole genome shotgun sequence.
ACCESSION   DASWNS010000132 DASWNS010000000
VERSION     DASWNS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160574"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VGB76_04385"
     CDS             <1..703
                     /locus_tag="VGB76_04385"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY0098174.1"
                     /translation="FKLAQELFGDWRKGDDPFVKYPMVEHPPLPKSEGAVIKQPVNNV
                     IINIGWHGPSIGKDNPATYAADVFSFILQQPDARFQRALVDTGLVTGVSFNYYTQRNV
                     GPIQILAQTTPDKARAAMRAIYKEVAAFNDPNYYTDEQLEAAKGLLEAEDLYSREKLS
                     EYTHTIGFWWASTGLDYFRGYLGNLRRTSRADITRYVTTYIQGKPHVGLALLSEESQQ
                     QAQLTPQDLIGEGAK"
     gene            796..2244
                     /locus_tag="VGB76_04390"
     CDS             796..2244
                     /locus_tag="VGB76_04390"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0098175.1"
                     /translation="MKMEIQNRKHSRRAFVLSMACAFMLFFAWGGETWAQQQPQQGGA
                     AASSSKAVQPAPDKEKVKEVAATAESLVTEFEVNGLKVLVKRRAGSLSVSAGLFMRGG
                     ARNVTAENAGIEALMLDAATEGSTGFPRETLRKELARMGTGISYGITYDYSVLALGST
                     RANFERSWEIFTDVALRPAFVPEDVRRVQNRLVISRRDDADTPDSFIQVLQSRVAYAG
                     HPYMNDPDGTEQTLGRLTVEDLRRYHAQTMQTSRLLLVLVGDLDAARVRESITASFGK
                     LPRGDYRPSPVPPLTFNTATVDVTQRAIPTNYIQGVFSAPPVAADDYYAMRVASSLLN
                     DRVLYEVRYKRNLSYAPEAFLRSQGANLGGIAVSAVDANQAISVMLYEISKMQREAAD
                     PTDINKVISGFLTQHYIGQETNAAQAGDLAMYELIGGGWRNSLTFLERIRAVTPKDVQ
                     RVAQKYMHHLRFVVLGDPARIDKNVFTVQLGG"
     gene            complement(2336..3520)
                     /locus_tag="VGB76_04395"
     CDS             complement(2336..3520)
                     /locus_tag="VGB76_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0098176.1"
                     /translation="MPKSQLPPIPRPLLTLLLCLLAFASASQSARAQQQQQTESLPEL
                     VRRVKPAVVAIVTYDAKGEPLLSGSGFFIRPGQVLTNLHVVEGARRVEVKTLDGKGRI
                     YASQGALDFDEEADLAVLSVDVPHERTRVIELTNSLPEEGEGVFVIGNPLRLEGSVSD
                     GIVSAVREVPNLGKIIQTTAPISHGNSGSPVFNLRGQVVGVVTVKVTNGQNINLALAS
                     ARLASLKPDKLTTFNALATRNRSTPGGDASAEWWYKNGLNSLWLGNYDGALGSFEQVV
                     NKNPNRVEAWIQVGYCKVKQGKMNDAIRAYKQALRLRPNSVEAYNKLGDAYYYAANFD
                     EAITAYKQAARLRPDLGEAFYNLGMTYLETGDRNAALAQSQLLQKLDAELYQKLASEL
                     RR"
     gene            complement(3876..4850)
                     /locus_tag="VGB76_04400"
     CDS             complement(3876..4850)
                     /locus_tag="VGB76_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098177.1"
                     /translation="MSKRLLCIAGSIALSLASATFVAAQETRPTTTTTTTQTTKTIQN
                     ADGSYTVIQYPADKEVTVDLMPGTLIPGAKGWAKVIRKGDATNINLDLSGITGDVNNF
                     NVYAVDPSGKFTLLGPVTVANGVATQSFTTPMDKFMLLLSPENNLTAMADNTPVLFRS
                     GVPQGFAVLPMGSRGERDGAAVGERVAAATTSGASPAYTAPLLGIPNWRQGTDTEMKI
                     NFAGEMTGTRANVSIEPRKDGATTIRMRFHELKEAPGGKRYVLWAISPDNKYHRLGQV
                     VNTGQRNEAEIRTETGLPDFGLFITTEDVNDAPPTGTIVGTIMRGGGK"
     gene            complement(5148..>6302)
                     /locus_tag="VGB76_04405"
     CDS             complement(5148..>6302)
                     /locus_tag="VGB76_04405"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY0098178.1"
                     /translation="SAAADLALASPQPAPETATSFVYSPDVDPTSPDFDTEAGAELSG
                     NAGTMVDNINRCLHEEMARDPRIVVFGEDVADVSREENLDKVKGKGGVFKVTANLQRK
                     FGGARVFNSPLAEANIVGRAIGMATRGLKPVVEIQFFDYIWPAYHQIRNELALMRWRS
                     ANDWKAPIVIRCPVGGYLKGGAVYHSQSGDTLFTHIPGLRVVYPSNALDANGLLRTAI
                     RSDDPVLFLEHKHLYRQAYNKSQYPPDNFLIPFGKAKTVREGTDLSIITYGALVQRSN
                     VAAKQAEAQGTSVEVIDLRTLAPYDWEAIVATVKKTSRVIVAHEDTRTHGFGAEIAAR
                     IGDELFEYLDAPVRRVAALDTFVAYAPQLEDHILPQPEDVLRAITELKAY"
BASE COUNT         1161 a         1829 c         2029 g         1283 t
ORIGIN      
        1 cttcaaactc gcgcaggagc ttttcggcga ctggcgcaaa ggtgacgatc cgtttgtgaa
       61 gtatccgatg gtcgagcatc cgccgctccc gaagagcgag ggcgcggtga tcaagcagcc
      121 cgtcaacaac gtcatcatca acatcggctg gcatggtccc tcgattggca aagacaatcc
      181 ggcgacttac gcggcggacg tgttctcttt catcctgcaa caacccgatg cgcgcttcca
      241 acgcgcgctc gtcgataccg gactcgtcac gggcgtcagc ttcaactact acacgcagcg
      301 caacgtcggc cccatccaga ttctcgcgca gacgacgccc gacaaggcgc gcgccgccat
      361 gcgcgcgatc tacaaagagg tcgccgcgtt caacgatccg aactattaca cggacgagca
      421 actcgaagcc gccaaaggtc tgctcgaagc cgaagacctc tactcgcgcg aaaaactgag
      481 cgagtacacg cacaccatcg gcttctggtg ggcttcgacc ggactcgact attttcgcgg
      541 ttatctcggc aacctgcgcc ggacttcgcg cgccgacatc acacgctacg taaccacata
      601 cattcaaggc aaaccgcacg tcggcctcgc gctgctctcc gaagagtcgc aacagcaggc
      661 gcaactgacg ccgcaggatt taatcggaga aggagcgaag tagtaagtga tgagtgatgg
      721 gtgatgagcg atgagtcaga accaaagact tgtctttcac tcatcactca tcactcatca
      781 ctcatcactt tttttatgaa gatggaaatt caaaatcgta aacattcgcg gcgcgccttt
      841 gtgctgtcta tggcgtgcgc ctttatgttg ttcttcgcgt ggggcgggga gacgtgggcg
      901 cagcagcaac cgcaacaggg aggcgcagcg gcttcgtcgt cgaaagctgt tcagcccgcg
      961 ccggacaaag agaaggtaaa ggaagtggcg gcgaccgccg agtcgctcgt caccgaattt
     1021 gaagttaacg ggttgaaggt gctggtcaag cggcgcgcgg gcagcctgtc cgtgtcagcg
     1081 gggctgttca tgcgcggagg cgcgcgcaac gtgacggcgg agaacgcagg catcgaagcc
     1141 ctgatgctcg acgccgcgac ggaaggcagc accggcttcc cgcgcgagac gctgcgcaaa
     1201 gaactcgcgc gcatgggcac gggcatcagc tacggcatca cctacgacta ttccgtgctc
     1261 gcgctcggct cgacgcgcgc caacttcgaa cgctcctggg aaatctttac agacgttgcg
     1321 ctgcgtcccg cgttcgtgcc cgaagacgtg cgacgcgtgc agaatcgcct cgtcatctca
     1381 aggcgcgacg atgccgacac gcccgactct ttcatccagg tgctgcaatc gcgcgtggcc
     1441 tacgccgggc atccttacat gaacgacccg gacggcacgg agcagacgct cgggcgtctg
     1501 accgtcgaag acctacgccg ctatcatgcg cagacgatgc aaacctcgcg gctgttgctg
     1561 gtcttggtcg gcgatctgga cgcggcgcgc gtgcgtgaga gcatcacggc ttcgttcggt
     1621 aaacttccgc gcggcgatta tcgcccgtcg ccggtgccgc cgctcacttt caacaccgcg
     1681 accgtcgatg tgacgcagcg cgccatcccg accaattaca ttcagggcgt cttttccgcg
     1741 ccgcccgtcg ccgcagacga ttactatgcg atgcgcgtcg cctcttcgct cctgaacgac
     1801 cgcgtgctct atgaagtgcg ttataagcgc aatctctctt acgcgcccga agcgttcctg
     1861 cgcagccagg gcgcgaacct cggcggcatc gccgtctcgg cggttgacgc caatcaggcc
     1921 atcagcgtga tgctctacga aatctcgaag atgcagcgcg aagcggctga cccgacggac
     1981 atcaacaaag ttatctccgg cttcctgacc caacactaca tcgggcagga gacgaacgcc
     2041 gcgcaggccg gcgacctcgc catgtacgaa ctcatcggcg gcggctggcg caactcgctc
     2101 accttcctcg aacgcatccg cgccgtcacc cccaaggacg tgcagcgcgt cgcccaaaag
     2161 tacatgcacc acttacgttt cgtcgtcctc ggcgaccccg cgcgcataga taagaacgtg
     2221 ttcacggttc aactcggagg gtaaagaaga aacgacgaag caggaacgat gaacgcggaa
     2281 tgaaagcatg ttgtctttca ttccgcgttc atcgttcctg cttcgtcgtt ttatctcagc
     2341 gtcgcagttc gctcgctaac ttttgataga gttcggcgtc gagcttctgt agcagttggc
     2401 tctgtgcgag ggcggcgttg cggtcgccgg tttcgagata ggtcatgccg aggttgtaga
     2461 aggcttcgcc gaggtctgga cgcaggcgtg cggcctgttt gtaggcggtg atggcttcgt
     2521 cgaagttggc ggcgtagtag taggcgtcgc cgagtttgtt gtaagcctct acggagttgg
     2581 ggcgcaagcg cagggcttgc ttgtaggcgc ggatggcgtc gttcatcttg ccctgtttga
     2641 ccttgcagta gccgacctgt atccacgctt cgacccgatt cggatttttg ttcaccacct
     2701 gctcaaacga tccaagcgcg ccgtcgtaat ttcccagcca cagggagttc agaccgttct
     2761 tgtaccacca ttcggcggaa gcgtcgccgc cgggcgtgct gcggttgcgc gtggcgagcg
     2821 cgttgaaggt cgtcaacttg tcgggcttga gcgaggcgag ccgcgccgag gcgagcgcga
     2881 gattgatgtt ctgcccgttc gtcactttga cggtgacgac gcccaccacc tgcccccgca
     2941 aattaaagac gggactgccg ctgttgccgt gcgagatggg cgcggtcgtc tggatgatct
     3001 tccccagatt cggcacttcg cgcacggcgg agacgatgcc gtccgacaca gacccttcga
     3061 gccgcagagg gttgccgata acaaacactc cctcgccttc ctccggcaaa ctgttggtca
     3121 actcgatgac gcgcgtgcgc tcgtgcggca cgtccacgct gagaaccgcg aggtcggcct
     3181 cttcgtcaaa atcgagcgcg ccctgcgagg cgtagattct gcctttgccg tcgagcgtct
     3241 tcacttcgac gcgccgcgcg ccttcgacga cgtgcagatt ggtcaagacc tgaccggggc
     3301 gtatgaagaa cccgctgccg ctcaggagcg gctcgccctt cgcgtcgtag gtgacgatgg
     3361 cgacaaccgc aggcttcacg cggcggacga gttccggcag cgactcggtt tgttgctgct
     3421 gttgggcgcg tgcgctttga gacgcagagg cgaaggcgag caggcagagc agaagcgtca
     3481 ggagcgggcg ggggattggg ggaagttggc ttttcggcat ggctttgttt ccggacaaga
     3541 cggcagggga gtcgtcgcgt cgtcgtgcgc ctccgaaaga gcggtgcggc gcagaatagt
     3601 ttacgaatta tctgagagct aagtttggtc tgacttaatt cttttaggtg gagactgacg
     3661 aggtatgccg tgtgtgtcgg ccaggcggct tttcggggaa ctactcaccg cctgttactg
     3721 ctgccgcccg tcgcgcgggc tacaagaaat atacagggcg ggcgactggc gggtcaataa
     3781 aaaaggctgc cctccgtgga aggcagcctg tcgaggcttg cgcgttgtag gtcgtagcga
     3841 gggctgcggg cgcaagcaaa gtttgaagag ctgttctact ttccgccacc gcgcatgatc
     3901 gtccccacga tggttccggt cggcggcgcg tcgttcacgt cctcggtcgt gatgaagagg
     3961 ccgaagtcgg gcaagcccgt ctcggttctg atctcggctt cgttgcgctg gcctgtgttc
     4021 accacctgac ccagacggtg atacttgttg tcggggctaa tcgcccagag cacgtagcgt
     4081 ttgccgccgg gagcttcctt caattcgtgg aagcgcatac ggatggtggt cgcgccgtcc
     4141 ttgcgcggtt cgatggagac gttggcgcgc gtgccggtca tctcgcccgc gaagttgatc
     4201 ttcatctcgg tgtccgtgcc ctgtcgccag ttcgggatgc cgaggagcgg cgcggtgtag
     4261 gccggtgacg cgccggaagt cgtcgccgcc gccacgcgtt cgccgacggc tgccccgtcg
     4321 cgttcgccgc gactgcccat cgggagcacg gcgaagccct gtggcacgcc gctgcggaag
     4381 aggacgggcg tgttgtcggc catcgccgtc aggttgtttt cgggtgagag caggagcatg
     4441 aacttatcca tcggcgtggt gaaactttgc gtcgccactc cgttcgccac cgtgacggga
     4501 ccgagcaatg tgaacttgcc cgacgggtcg acggcgtaga cgttgaagtt gttcacgtcg
     4561 ccggtgatgc ctgagaggtc taaattgatg ttcgtggcgt cgcccttgcg gatgactttc
     4621 gcccagccct tcgcgccggg aatgagtgtg cccggcatca ggtcaaccgt cacctctttg
     4681 tcggctggat actgaatcac ggtgtagctg ccgtcggcgt tctgaatggt cttggtggtt
     4741 tgtgtcgtgg tagtggtcgt ggtcgggcga gtttcttgcg ccgcgacaaa agtggcggaa
     4801 gcaaggctca aggcgatgct gcccgcaatg caaagcaaac gtttactcat gttggaattt
     4861 ctcctccgga ttttaagttg ttgactgagg gggaggaagt ttcaaatagg ggactcactt
     4921 gaaccctgag tggcaaccac catgccgcgc cgcattcgcc tctacttccg aaagaatgga
     4981 taaaaaggcc gaaaaaacgt ccccaaaagc ggaacgttac agtacaggcg tgtatgacta
     5041 acgattaaag gaaggggcgg cgggaatagt gataagaggt tattaatgcc caggggcggt
     5101 cgtgttgagc gaccgcccct ttagtttttg agaactgtgc gcctgtgtca gtaggctttg
     5161 agttcggtga tggcgcggag cacgtcttcg ggttggggga ggatgtggtc ttcgagttgg
     5221 ggcgcgtagg cgacgaaggt gtcgagggcg gcgacgcggc ggacgggggc gtcgaggtat
     5281 tcgaacagtt cgtcgccgat gcgcgcggcg atctccgcgc cgaagccgtg cgtgcgggtg
     5341 tcttcgtgcg cgacgatgac gcggctcgtc ttcttcacgg tggcgacaat cgcttcccag
     5401 tcgtaggggg cgagcgtgcg caggtctatc acttcgacgc tcgtgccctg cgcctcggcc
     5461 tgcttggcgg cgacgttgga gcgttggacg agtgcgccgt aagtgatgat cgagaggtct
     5521 gtgccttcgc gcacggtctt ggccttgccg aaggggatga ggaagttgtc gggtggatac
     5581 tgcgatttgt tgtacgcctg acggtagaga tgtttgtgtt cgagaaagag cacgggatcg
     5641 tctgagcgga tcgccgtgcg cagcaggccg ttggcgtcga gcgcgttcga cgggtagacg
     5701 acgcgcaggc cggggatgtg cgtgaagagc gtgtcgcccg attgcgagtg atagaccgcg
     5761 ccgcccttga ggtagccgcc gacggggcag cggatgacga tgggcgcttt ccaatcgttg
     5821 gcggaacgcc agcgcatgag cgcgagttcg tttctgatct ggtgataggc aggccagatg
     5881 tagtcgaaga attgaatctc gacgacgggt ttcaaaccgc gcgtcgccat gccgatggcg
     5941 cgcccgacga tgttggcctc cgcgagcggc gaattgaaga cgcgcgctcc gccgaacttg
     6001 cgttgcagat tcgccgtcac tttgaagacg ccgcccttgc ccttcacctt atccagattc
     6061 tcttcgcgcg acacgtcggc cacgtcttcg ccgaagacga cgatgcgcgg atcgcgcgcc
     6121 atctcttcat gcaggcaacg gttaatgttg tcaaccatcg tgcccgcgtt gccggagagt
     6181 tccgcgcccg cttccgtgtc gaagtcgggc gacgtgggat caacgtcggg cgaatagacg
     6241 aaagatgtcg ccgtctcggg cgcgggctgc gggctggcga gcgcgaggtc ggcggcggcg
     6301 ct
//
LOCUS       DASWNS010000133        13116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_264601, whole genome shotgun sequence.
ACCESSION   DASWNS010000133 DASWNS010000000
VERSION     DASWNS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13116
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264601"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1187)
                     /gene="asd"
                     /locus_tag="VGB76_04410"
     CDS             complement(132..1187)
                     /gene="asd"
                     /locus_tag="VGB76_04410"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY0098179.1"
                     /translation="MSKKFRVGILGATGTVGQRFIELLEGHPQFEVNALAASDRSQGK
                     TYREACAWRLAGECPARVAALKVSAPAPPLDCDLIFSSLPGEMAREAEDAFARAGYPV
                     ISNSSAFRMDEDVPLLIPEINHEHLALIETQQRKRGYERGFLVTNPNCSTIMIALALA
                     PLHARFGIESCIATTLQALSGAGYPGVASLDVLDNVLPYIASEEEKIESETLKILGRM
                     AGACVEPAEMRVSAQCHRVNVSDGHLAALRVKFARPVELEELTDALASFRALPQELKL
                     HSAPARPIVVRDEPDRPQPRLDRDAGRGMSVTVGRIRRDAVLDYRFVALSHNTVRGAA
                     GAAILNAELLAARGRLV"
     gene            complement(1300..2487)
                     /locus_tag="VGB76_04415"
     CDS             complement(1300..2487)
                     /locus_tag="VGB76_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0098180.1"
                     /translation="MESNFNAAHDFQPARRLRGIEKSMIRQLFDRARAGSINLGLGEP
                     DLPTPEVIRRAAVRVIQEEQNGYTSHAGLPALRERVAADYTQLSASGTGTTPDNVIIT
                     AGSQEALYLALMTLVDEGDEVLLPDPGFVAYPTIVRMAGGRPIFYRLPASGGFAFDAE
                     DFRRRLTPRTKVVVCISPSNPTGRTLAPKELEAIAGALRDSDAYVISDEIYREIYFGD
                     VRPASISEHTPARRTIVVGGLSKSMSMTGWRVGWMCGAPEVVKSALVLHGYVTTCAST
                     VSQKAALAAWTVEAAAAREETRRIFRTRRDELLSLIARELNLAAVAPDGAFYLMLDVS
                     AHGSSLEVAERLLEHGVITVPGSAFGTEGEGYLRLSFCAETPLLAEGVKRIKEALKAE
                     AKG"
     gene            complement(2487..2846)
                     /locus_tag="VGB76_04420"
     CDS             complement(2487..2846)
                     /locus_tag="VGB76_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098181.1"
                     /translation="MLVWNEYEFIECLGVIPKVEEYETAHFFKVSKDGLRLELSVFQY
                     DGDVYFDLYREGIESPVLQVRLLNCAGARYVSHKNGREMLEFAPAKSFGSRYDGVSPL
                     PYGIRVAVTPHISIELF"
     gene            complement(2859..3701)
                     /locus_tag="VGB76_04425"
     CDS             complement(2859..3701)
                     /locus_tag="VGB76_04425"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY0098182.1"
                     /translation="MTTLRERIETLAAKEAGSSFTEAEFALFAEFKSALNRGEARAAE
                     RDADGSWKVNAWVKRGILLGFRIGTLADMSPRDHTLRFFDKDTYPVHQFTTTDGVRLV
                     PGGSSVRDGAYLAPGVVCMPPMYVNVGAYVDEGTMIDSHALVGSCAQVGKRVHLSAAA
                     QVGGVLEPVGASPVVIEDDVLVGGGCGIYEGAIVRERAVLAAGTILTGSTPVYDLVRE
                     QVYRRTEDAPLEIPAGAVVVPGSRAVTSERGREWGLSLYAPVIVKYRDEKTDRAVQLE
                     DYLR"
     gene            complement(3727..4644)
                     /gene="dapA"
                     /locus_tag="VGB76_04430"
     CDS             complement(3727..4644)
                     /gene="dapA"
                     /locus_tag="VGB76_04430"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY0098183.1"
                     /translation="MTLKVEWLRGCATALVTPFKGGGAIDEERLTALVERQIAGGVRL
                     LVPCGTTGESATLTEEEDRLVIRLTVEAARGREARVIAGTGSNSTAAAIEYARAAREI
                     GADAVLVVAPFYNKPTQEGLYAHFRAVAEAVAGLPVVIYNVPGRTASNITAPTALRLA
                     RDVENIVAVKEASGDLSQIMSILRERPENFRVLSGDDALTLPMLALGADGLISVASNE
                     TPELMSRLCELALAGSWEEARALHFRLLPLMEVNFIESSPGPVKAAMHMLNLLEENFR
                     LPLVPVQEKTRARVREVLAELGLLQVSQA"
     gene            complement(4799..5500)
                     /locus_tag="VGB76_04435"
     CDS             complement(4799..5500)
                     /locus_tag="VGB76_04435"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0098184.1"
                     /translation="MRLAIIGNGAMGKLVRALAETEGHEVARVFTSRDSSLSPDELSE
                     ALRGHDAAIDFSVAGAVLKNIEAAARAGVPLVEGTTGWNEHEAEARRIVASHNGALLY
                     GANFSIGVNLFYRIVARAAELFAATDYAPFIEEAHHARKRDAPSGTALKLRDVLAAHL
                     ARDIPVASTRAGHIPGTHTIGFDAAADQITLTHTARTREGFASGSLLAARWLKDRHGV
                     YEFSDVIDKIVTSDK"
     gene            complement(5497..6576)
                     /locus_tag="VGB76_04440"
     CDS             complement(5497..6576)
                     /locus_tag="VGB76_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0098185.1"
                     /translation="MNLFALTRRLIDIPSPSGEEDAATRFLAAHLEKLGYRVELQEVA
                     PQRANIIATASTAPRLFFSTHIDTVPPVITSSEDETYIYGRGSCDAKGIIAAQIFAAE
                     RLRLEGVEEVGLLFTVDEELGSLGARAANAHPLASTCAYLINGEPTENRLAAGSKGSL
                     RLRLSTVGRAAHSAYPEHGDSAIERLLDVLEDVRRSAWPQDDFFGATTCNIGTIEGGT
                     RPNVIPAAASAELQIRLVAESARVKAELERTVAGRAQIEYLSVAEPVRLYAPEGFARE
                     VVRFTTDIPYLPNWGTPLLLGAGSILDAHTAGERIGKDELTRSVELYVRLARELLARR
                     SSSDAESEGQSVPGQDTAVQGASLK"
     gene            complement(6573..7988)
                     /gene="lysC"
                     /locus_tag="VGB76_04445"
     CDS             complement(6573..7988)
                     /gene="lysC"
                     /locus_tag="VGB76_04445"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HEY0098186.1"
                     /translation="MADQAIVMKFGGTSVEDAQAFARVARIVGARRKERPVVVVSAMS
                     RVTDALLAGVEMAAAGDAEAARRSLEEHFERHLLVARTLPAGARDEFFVELASAERTL
                     AELLKRVERAARELRPSLQDQIVSYGECLSARLLAAVLSERALPAAYVDARRCIITNE
                     DHGCATPANEETYRHTRAELSPLLDAGRIPVLGGFIGSTPGGQTTTLGRGGSDYTAAL
                     IGAGIGAREIQIWTDVNGVLTADPRVVAHARTIPSLSFAEAAELAYFGAKVIHPKTIL
                     PAVIRSIPVRICNSRDADHPGTGVYFDAEMSPRTVKAIAHKKGITVLHITSARMLGAY
                     GFLRALFEVFERHRTIVDIVTTSEVSVSLSLEDTSTIDEIIKDLRAIAAVKVETNLAV
                     VCIVGEELRYTSGIAARIFSTLRDINVLLISQGASNVNLTFVVAERDVEETVRLLHAS
                     LFECDTQELAPLFQRREGVSQ"
     gene            complement(8179..8249)
                     /locus_tag="VGB76_04450"
     tRNA            complement(8179..8249)
                     /locus_tag="VGB76_04450"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8215..8217),aa:Gln,seq:ctg)
     gene            complement(8414..9040)
                     /locus_tag="VGB76_04455"
     CDS             complement(8414..9040)
                     /locus_tag="VGB76_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098187.1"
                     /translation="MFLNTLASSPHQITPIASILRFSSLLRCPTTNPELSPSIERQFM
                     LKIISLILVFSLTLGTTTAFAKSDSASYYGGTMVAFKDAKKEVKGKLITTDEKDLQFL
                     YGKGQVVHIPYANFLDIEYGQKSGRRVGAAVATTILLGPLGLLTLFSKKQNHFLTLGF
                     KDDEGKDQVAVFKINKDMVRTILPILEARSGKKVEYQSKGAEKKATGK"
     gene            complement(9063..10922)
                     /locus_tag="VGB76_04460"
     CDS             complement(9063..10922)
                     /locus_tag="VGB76_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0098188.1"
                     /translation="MNRALPEGEAIRPQWAPGPLLKSYEKTAPPLGFPRETDSLCPTC
                     VKEVREAVISGQTPLETLMHEHPGEIKAQIVEEDGRILMKKTCVKHGEFVDVMATSPE
                     FLQRIESLFYGRDFRAAEDAHVHRHGTSDIKFGRGAVLTVDLTNRCNMMCNPCFMDAN
                     QVGYVHEPTYEDTKAILDRAVSFKPKRQVIILFSGGEPTLSPYYLEAVAYAKKVGFYR
                     ILAATNGIRFAEDIEFCRQAKEAGQHGVYLQFDGVSEEKNKHRGVGNLFDVKQKAIEN
                     LAEVGIKVTLVTTIVNTINNDAIGQIVEFAAKNIDKVQTIAFQPVSFTGRDEDVPDDV
                     RIKQRYTLADMATDLETQLGGDLKALRDWFPLSSYSAFTSVMDMLQGADAPWGWSSCN
                     CHPNCGVFTLMVVNRHTGAWTPLFKFFNYEQFMKDVAVITDTARGAKLSMAQLAMTIM
                     RNFDAKSAPEGFPISQIINLFKPSSTNSNSDRNDRMKTQHGSEDPNDAWRVLCVEGMW
                     FQDLFNYDFRRTEMCVIPYGTQEGEISFCAYNTGVGWRQIIENMHKTAGLAEWYEEHG
                     RHAVYAKGKEVPGIPKKHSLSLPIVQAILAEDADEKPVKVSPYLVEEEEEAVV"
     gene            complement(11806..>13116)
                     /locus_tag="VGB76_04465"
     CDS             complement(11806..>13116)
                     /locus_tag="VGB76_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098189.1"
                     /translation="HNGFANVLNYLVAAEYREDYIDRALMEYTAAGFYFEKVGHARYQ
                     ACVENNLGFLYGTISRFAEAHEHLDRAQALFTSLKDRVHLAQVDETRAKVLLKQRREV
                     EAEKLANSAVRVLEKGDEHSLYVEALITHGIALARLGRAGNAYEALKKAIKVAEEAGD
                     LESAGQAAMTIIEECGSHLSRSELSEIYVRADELLGKSQNMANLKRLCACARRVLFST
                     HLSPTPHDWTGFSFKDAVRRYESHLIERALKDADGMVSRAAQLLGVKHHQSLVSLLKN
                     RHKHLLHVRTPIVPRKRSIIRALDPCRAREQAGEKAARSVVILHVEDNGQLAEAVRET
                     LCEEGWTVETCADGVDALHQLAGETRYDLLLFDNELPGASGIELVRRARGLPHRRQTP
                     IIMFSASDCEENARRAGVDAFLRKPQDMARLVEAVARLLAEKSA"
BASE COUNT         2465 a         4033 c         3997 g         2621 t
ORIGIN      
        1 gaagtgatca attttatgaa ggcgggcgcg ggagcgagtc gttagccgca gcgttagccg
       61 cagtttcaaa agtcacgccg ggattgcgaa gcccgcgtca tgacttcgca aacttctgct
      121 tctaacttct ttcagacgag gcggccacgc gcggccagca actccgcgtt gaggatggct
      181 gcgcccgccg cgccacgcac ggtgttgtgg ctgagcgcga cgaagcgata atccagcacc
      241 gcatcgcgcc ggatgcgacc gacggtgacg ctcattcccc gtccggcgtc gcggtcgagt
      301 cgcggctgcg ggcggtcggg ttcgtcgcgc acgacgatgg ggcgcgcggg cgcggagtgg
      361 agtttgagtt cctgcgggag cgcgcgaaac gaagcgagcg cgtccgttaa ttcttccaac
      421 tcaaccgggc gcgcgaactt gacgcgcaac gccgcgaggt gtccgtccga cacgttgacg
      481 cggtgacact gcgcgctcac gcgcatctct gcgggttcga cgcacgcgcc tgccatgcgt
      541 cccaaaatct tcaacgtctc gctctcaatc ttctcctctt cgcttgcgat gtagggcagc
      601 acgttatcca acacgtcgag cgaggcgacg cccggatagc ccgcgccgga caaagcctgc
      661 aacgtcgtcg caatgcacga ctcgatgccg aaacgcgcat ggagcggtgc cagcgcgagc
      721 gcgatcatga tggtcgagca gttcgggttg gtgacgagga agccgcgctc gtagccgcgc
      781 tttcgctgtt gcgtctcgat gagcgcgaga tgctcatggt tgatctccgg gatcaggagc
      841 ggcacgtcct catccatgcg aaaggcggag gagttgctga tgacggggta gcccgcgcgt
      901 gcgaaagcgt cttccgcctc gcgcgccatc tcgccgggca ggctggaaaa gatgaggtcg
      961 caatcgagcg gcggagcggg cgcggaaact ttcagcgcgg ccacacgcgc cggacactct
     1021 ccggcgagac gccacgcgca cgcttccctg taagttttcc cctgcgaacg atccgacgcc
     1081 gccagcgcgt tcacttcaaa ctgcgggtgg ccttccagca gttcgatgaa acgctgcccg
     1141 accgtgcccg tcgcacccaa tatgccgacg cgaaattttt tactcataga aaatctccag
     1201 acaggaagag cggaggccgg agcttgaagt ttagaaagaa tctgccccgg tctttcactg
     1261 atctctaact tctaaccttt aacctctaac ctttaacctc taacctttgg cctctgcttt
     1321 taacgcttcc ttgattcgct tgacaccttc cgcgagcagt ggcgtctcgg cgcagaagga
     1381 gagacgcaag tagccttcgc cctctgtgcc gaaggcggaa ccggggacgg tgatgacgcc
     1441 gtgctcaagc agccgctccg ccacctccag agaacttccg tgtgcgctca cgtcgagcat
     1501 cagatagaac gcgccatcgg gcgcaaccgc cgccaagttc aactcgcgcg cgatgagcga
     1561 caggagttcg tcgcggcgcg tgcgaaagat gcggcgcgtc tcttcgcgcg cggcggcggc
     1621 ctccaccgtc cacgcggcga gcgcggcctt ttgcgagacg gtcgaagcgc aggtcgtgac
     1681 ataaccgtgc aggacgagcg cgctcttgac gacctccggc gcaccgcaca tccagccgac
     1741 ccgccatccc gtcatgctca tagatttcga gaggccgccg acgacgatgg tgcggcgcgc
     1801 aggcgtgtgc tcggagatgg atgccgggcg cacatcgccg aaatagatct ctctgtaaat
     1861 ctcgtcgctg ataacgtaag cgtcgctgtc gcgaagcgca ccggctatcg cctcaagttc
     1921 ctttggcgcg agcgtgcgcc cggtcgggtt tgaagggctg atgcagacta cgactttcgt
     1981 gcgcggcgtg agacgacgac gaaagtcttc cgcgtcgaac gcgaacccgc cgctcgcggg
     2041 caggcgataa aagatcggac gccctccggc cattcggacg atggtcgggt aggcgacgaa
     2101 accgggatcg ggcaggagca cctcgtcgcc ttcgtccacg agcgtcatca gtgcgagata
     2161 gagagcctct tgcgacccgg ctgtgatgat cacgttgtcg ggcgtcgtgc ctgtccccga
     2221 cgccgaaagt tgcgtgtagt ctgcggcgac gcgttcgcgc agagcgggca gaccggcatg
     2281 tgaagtgtag ccgttctgtt cctcctgaat gacgcgcacg gcggcgcggc gaatcacttc
     2341 gggcgtcggc aggtctggtt cgccgaggcc gagattgatg ctccctgcgc gcgcgcggtc
     2401 gaagagctgg cgaatcatgc tcttctcgat gccgcgcagg cggcgcgcgg gctgaaaatc
     2461 gtgtgcggcg ttgaagttgc tttccatcaa aatagttcga ttgagatgtg cggcgtgacc
     2521 gcgacgcgaa tcccgtacgg cagaggcgag acgccgtcgt agcggctgcc gaaactttta
     2581 gctggcgcga attcgagcat ctcgcgcccg ttcttatggc tgacgtatcg cgctccggcg
     2641 cagttcagca ggcgcacctg taagacgggc gattcgatgc cctcccggta caagtcgaaa
     2701 taaacgtcgc cgtcatactg gaaaacgctc aactccaaac gcagtccgtc cttcgagact
     2761 ttgaagaaat gagccgtctc atattcttcg accttcggga taactccgag gcactcgata
     2821 aactcgtact cgttccaaac cagcatcggc gttcgacctc aacgcaggta gtcctccaac
     2881 tgcacggcgc ggtctgtctt ctcgtctcga tatttgacga tcacgggcgc gtagagcgac
     2941 agcccccact cgcgcccgcg ctcgctcgtg acggcgcgcg agccgggaac gaccactgcg
     3001 cccgcaggga tttcgagcgg cgcatcctcc gtgcggcgat agacctgttc gcgtacgaga
     3061 tcatagacgg gcgtggagcc tgtcaggatc gtgcccgccg cgagcaccgc gcgctcgcgc
     3121 acaatcgcgc cttcgtagat gccgcacccg ccgccgacga gcacgtcgtc ttcgatgacg
     3181 accggggatg cgccgacggg ttcgagcacg ccgcccacct gcgccgccgc cgacaggtgc
     3241 acgcgcttcc ccacctgcgc gcacgaaccg acgagcgcgt ggctgtcaat catcgtcccc
     3301 tcatcgacgt atgcgccgac gttgacgtac atcggcggca tgcagacgac gcccggcgcg
     3361 agatatgcgc cgtcgcggac gctcgatccg ccgggcacga gtcgaacgcc gtcggtcgtc
     3421 gtgaattgat ggaccgggta agtgtcttta tcgaagaagc ggagcgtgtg atcgcgcggc
     3481 gacatgtctg cgagcgtgcc tatgcgaaag cccaagagga tgccgcgctt gacccacgcg
     3541 ttgaccttcc acgagccgtc tgcgtcgcgt tcagccgcac gcgcctcgcc ccggttgagc
     3601 gcgcttttga attcggcgaa cagggcaaac tctgcttccg taaaactgct gcccgcctct
     3661 ttcgccgcga gcgtttcgat gcgttcacgc aaagttgtca tcaagcgttt ccctcgacaa
     3721 attccctcaa gcctgcgaaa cctgcaacag cccgagttca gccagcacct cacgcacgcg
     3781 cgcacgcgtc ttctcctgaa cgggcacgag cggcaatctg aaattctctt ccagcaggtt
     3841 caacatgtgc atggccgcct tcaccggacc cggactcgac tcgatgaagt ttacttccat
     3901 caaaggcaac aggcggaagt gtaacgcacg cgcctcctcc caacttccgg cgagcgcgag
     3961 ttcgcacagc cgcgacatca actccggcgt ctcgttcgac gcgacggaga tcaatccgtc
     4021 cgcgccgagc gcgagcatcg gcagcgtcaa cgcatcgtcg cccgaaagca cgcgaaaatt
     4081 ttcgggacgc tcgcgcagta tcgacatgat ctgcgagagg tcgccggagg cttctttgac
     4141 ggcgacgata ttctccacgt cgcgcgcgag ccggagcgcg gtcggggcgg tgatgttcga
     4201 ggcagtgcga cccggcacgt tgtagatgac gacgggcaaa ccggccaccg cttcggctac
     4261 cgcgcgaaag tgcgcgtaga gtccctcctg cgtcggcttg ttgtagaagg gcgcgacgac
     4321 gagcacggcg tccgctccga tctctctcgc cgcgcgggcg tattcaatcg cggcggcggt
     4381 cgagttgctg cccgtgcccg cgatgacgcg cgcttcacgc ccgcgcgcgg cttcgaccgt
     4441 caggcggatc acgaggcggt cttcttcctc cgtcagcgtc gcgctctcgc cggtcgtgcc
     4501 gcacggcacg agcaggcgca cgccgcccgc gatctgtcgc tcgacgagag ccgtcagccg
     4561 ttcctcgtca atcgccccgc cccccttgaa aggcgtcacc aacgccgtcg cgcacccgcg
     4621 cagccattca actttgagtg tcatcgctac ctctctcaag cagtgacaag tgacgggtga
     4681 ggagaaagaa aagtgatgag tgatgagtaa tgagtgatga gtaaaaaccc aagacttgtc
     4741 tttcactcat cactcattac tcatcactca tcactcatca ctcattactc atcacttctt
     4801 acttgtcact tgtcactatc ttgtctatca cgtcggaaaa ttcgtaaacg ccgtgtctgt
     4861 ctttgagcca gcgcgcggcg aggaggctgc cggaggcgaa gccttcgcgg gtgcgcgcgg
     4921 tgtgcgtcag agtgatctgg tctgccgccg cgtcgaagcc gatggtgtgt gtgccgggga
     4981 tgtgcccggc gcgcgtgctg gcgacgggga tgtcgcgcgc aaggtgcgcg gccaacacgt
     5041 cgcgcagctt gagagccgtg ccggaaggcg cgtcgcgttt gcgcgcgtgg tgcgcctctt
     5101 cgatgaaggg cgcgtaatcg gtcgcggcga agagttcggc ggcgcgtgcg acgatgcggt
     5161 agaagagatt gacgccgatg gagaagttcg cgccgtagag aagcgcgcca ttgtgagatg
     5221 cgacgatgcg acgcgcctcc gcttcgtgct cgttccagcc ggtagtgcct tcaaccagcg
     5281 ggacgcccgc gcgcgccgcc gcttcgatgt ttttgagcac cgcgcccgcg acggagaagt
     5341 ctatcgccgc atcgtggccg cgcaaggctt cggagagttc gtccggcgac agagaagagt
     5401 cgcgcgaagt aaacacgcgt gcgacttcgt gcccttccgt ctccgcgagc gcgcggacga
     5461 gtttgcccat cgcgccattg ccgataatgg cgagtctcac ttgagtgacg ctccttgaac
     5521 agccgtgtct tgccccggca ccgattgccc ttcgctctcc gcgtcgctac tgctgcggcg
     5581 cgcgagcaac tcgcgtgcga gccggacgta gagttcgacc gaacgggtca gttcatcctt
     5641 gccgatgcgc tcgcctgcgg tgtgcgcgtc gaggatggaa cctgcgccga gcaggagcgg
     5701 cgtgccccag ttcgggagat agggaatgtc tgtggtgaag cgcaccacct cgcgcgcaaa
     5761 cccttccggc gcgtagaggc gcacgggttc ggcaaccgag agatactcta tttgcgcgcg
     5821 ccccgcgacc gtgcgctcaa gttctgcttt gacgcgcgcc gattcggcga cgaggcggat
     5881 ttgcagttcg gcggaggctg cggcggggat gacgttggga cgcgtgccgc cttcgatggt
     5941 tccgatgttg caggtggttg cgccgaagaa gtcgtcctgc ggccacgcgc tgcggcgcac
     6001 gtcttcgagc acgtcgagca ggcgttcgat ggccgagtcg ccgtgttcgg gataagcgga
     6061 gtgggcggcg cggcctacgg tgctgagccg gaggcgcaga gagcctttcg atccggcggc
     6121 gagcctgttt tcggtcggct cgccgttgat gagataggcg caggtggagg cgagcgggtg
     6181 tgcgttcgcg gcgcgcgcgc cgaggctgcc gagttcttca tccacggtga agagcagccc
     6241 cacctcttcg actccttcaa gacggagacg ttcggcggcg aagatttgcg cggcgatgat
     6301 gcctttcgcg tcgcaggagc cgcgcccgta aatgtacgtc tcgtcttcgc tcgaagtgat
     6361 gacgggcggc accgtgtcga tgtgcgtgga gaagaagagg cgcggcgcag ttgaagccgt
     6421 agcgatgatg ttggcgcgtt gcggcgcgac ctcttgaagt tcgacccggt agcccaactt
     6481 ttcgagatgc gcggcgagaa atcgtgtggc cgcgtcctct tcgccgctcg gcgaaggaat
     6541 gtctatgagc ctgcgcgtga gggcgaagag attcattgcg agacgccctc cctgcgctgg
     6601 aagagcggcg ctaactcctg cgtgtcgcac tcgaagagcg aggcgtgcag gagtcgcacc
     6661 gtctcttcca cgtcgcgttc ggccacgacg aaagtgagat tgacgtttga cgcgccctgc
     6721 gagatgagca ggacgttgat gtcgcgcagc gtgctgaaga tgcgtgcggc gatgccggac
     6781 gtgtagcgaa gttcttcgcc gacgatgcag acgaccgcga gattggtttc aactttaact
     6841 gccgcgatgg ctcgtaagtc tttgatgatc tcgtcaatcg tcgaagtgtc ttcgagcgag
     6901 agcgagacgg agacttcgga ggtggtgacg atatcgacga tggtgcggtg gcgttcgaag
     6961 acttcgaaga gggcgcgcag gaaaccgtat gcgccgagca tgcgcgcgga ggtgatgtgg
     7021 agaacggtga tccctttttt gtgcgcgatg gctttgacgg tgcgcggcga catctccgcg
     7081 tcgaaataga cgcccgtgcc cggatgatcc gcgtcgcgcg aattgcagat gcgcacgggg
     7141 atgctgcgaa tgacggcggg caggatcgtc ttcgggtgga tgactttcgc gccgaagtag
     7201 gcgagttccg ccgcttccgc gaaagagagg ctggggatgg tgcgcgcgtg cgcgacgacg
     7261 cgcgggtctg cggtcaggac gccgttcacg tccgtccaga tttgaatttc acgcgcaccg
     7321 attcccgcgc cgatgagcgc agccgtgtag tcggaaccgc cgcgcccgag cgtggtcgtc
     7381 tgcccgccgg gcgtcgagcc gataaagccg ccgagcaccg gaatccgccc cgcatcgagc
     7441 aggggtgaga gttcggcgcg cgtgtgtctg taagtctctt cattcgcggg cgtggcgcag
     7501 ccgtgatctt cgttggtgat gatgcagcgg cgcgcatcga cgtaggcggc ggggagcgcg
     7561 cgttcggaga ggacggcggc gagtaatctg gccgagagac attcgccgta agagacgatc
     7621 tgatcttgca gcgacgggcg cagttcgcgc gcagcgcgct cgacgcgctt gagcagttct
     7681 gcgagcgtgc gttcggcgct ggcgagttcg acaaaaaact catcgcgtgc gcctgcgggc
     7741 agagtgcgtg cgacgagcag gtggcgttca aaatgttctt cgagcgagcg gcgcgcggcc
     7801 tctgcgtcgc ccgccgctgc catctcgacg cccgccagca gcgcgtccgt cacgcggctc
     7861 atggcagaga cgacgacgac gggacgctcc ttcctgcgcg cgcccacgat gcgcgccaca
     7921 cgcgcaaacg cctgcgcgtc ttcgacggac gtgccgccga acttcatcac gattgcttga
     7981 tcagccaata tctttcactc atccggcgcg acgcagcgcg ctcgacgggg acgactcaga
     8041 atgttcgatc aatttagagg gggcggagca ttataaggca tgaagcgcgt ccactgatac
     8101 gcgcgggcgt cctgcgggca aaatccgtgg caaaaattat taaggccgga cgcgtgcgcc
     8161 cggcctttga gttgattact gggagacagg gactcgaacc ccgaaagcct gatccagagt
     8221 caggtgtgat accatttcac catctcccaa cagcgatgat ggacaaaatt aacgtgcggg
     8281 cgcggcggtt gtcaaccgcg cctccgagcg gcttggcagg gacagtcggg ctagtatctg
     8341 gaatttggcg gttgcgacta aaggagactc gaaccgtgag gtggtcgagc ctcctcgcac
     8401 cgttaggctt gtcctactta ccggttgcct tcttctctgc tcccttactc tggtattcga
     8461 cctttttacc tgagcgggct tcgagaatcg gcaggatggt gcgtaccatg tccttgttga
     8521 ttttgaagac ggcgacctga tctttcccct cgtcgtcttt gaatccaagt gtcaggaagt
     8581 gattctgctt ctttgaaaag agcgtgagca gtccgagggg gccaagcagg atggtggtag
     8641 caacagcagc tccaactcta cgacccgact tctgcccgta ctctatatca aggaagttgg
     8701 cgtaagggat gtgcactact tggcctttgc catagaggaa ttgcaggtct ttttcatcag
     8761 tggtgatgag tttccctttt acctcctttt tggcgtcttt gaaggcgacc attgtgccgc
     8821 cgtaataaga agcactgtcg gatttggcga aggcagttgt tgtgcctaaa gttaaagaga
     8881 agacgagtat cagcgagatt atttttaaca tgaactgcct ttcgatggag ggtgagagtt
     8941 cggggttggt ggtcggacac ctcagtaaag aactgaagcg cagaattgac gcgatgggtg
     9001 ttatttgatg gggcgagctg gcgagcgtgt tgaggaacat gctcgccagc tcggaaaatt
     9061 ttttaaacta ctgcttcctc ttcttcttcg acgagatacg gcgacacctt cacgggcttc
     9121 tcgtcggcgt cctcggcgag gatggcctgc acgatgggga gcgagagcga gtgcttcttg
     9181 gggatgccgg ggacttcctt gcctttggca tagacagcgt ggcgtccgtg ctcttcgtac
     9241 cactcggcga gtccggcggt cttgtgcatg ttctcgatga tctgtcgcca gccgacgccc
     9301 gtgttgtagg cgcagaacga aatctcgcct tcctgcgtgc cgtaggggat aacgcacatc
     9361 tccgtgcggc ggaagtcgta gttgaacagg tcttggaacc acatgccttc gacgcagagg
     9421 acgcgccacg catcgttcgg gtcttccgaa ccgtgttgcg tcttcatgcg gtcgttgcga
     9481 tccgagttcg agttggtgga agacggcttg aagagattga tgatctgcga aatggggaag
     9541 ccttcgggcg cgctcttggc gtcgaagttg cgcatgatgg tcatggcgag ttgcgccatc
     9601 gagagtttcg cgccgcgcgc cgtgtccgtg atgacggcca catccttcat gaactgctcg
     9661 tagttgaaga acttgaagag cggcgtccat gcgccggtgt ggcggttgac gaccatcagc
     9721 gtgaacacgc cgcagttcgg gtgacagtta caactcgacc agccccacgg cgcatccgcg
     9781 ccttgcagca tatccatcac ggaggtgaag gccgaatagg acgagagcgg gaaccaatcg
     9841 cgcagggctt tcaagtctcc gccgagttgc gtttcgaggt cggtcgccat gtctgcgagc
     9901 gtgtaacgct gcttgatgcg cacgtcgtcg ggcacgtctt cgtcgcgccc cgtgaaggag
     9961 acgggctgga aggcgatggt ctgaaccttg tcgatgttct tcgcggcgaa ctcgacgatc
    10021 tggccgatgg catcgttgtt gatcgtattc acgatggtcg tcacgagcgt gaccttgatg
    10081 ccgacttccg cgaggttctc gatggccttc tgcttcacgt cgaagagatt gccgacgccc
    10141 ctgtgcttgt tcttctcttc gctcacgccg tcgaattgca ggtagacgcc gtgctgcccg
    10201 gcctctttcg cctgacggca aaactctatg tcttcggcga agcggatgcc gttcgtcgcg
    10261 gcaaggatgc ggtagaagcc gaccttcttc gcgtaagcga cggcttcgag atagtagggc
    10321 gaaagcgtcg gctcgccgcc ggagaagagg atgatgacct gacgcttcgg tttgaaggag
    10381 acggcgcggt cgagaatcgc cttcgtgtct tcatacgtcg gctcgtgcac gtagccgacc
    10441 tggttggcgt ccatgaagca cgggttgcac atcatgttgc agcggttcgt gaggtcaacc
    10501 gtcaacacgg cgccgcgccc gaacttgatg tctgacgtgc cgtgacgatg gacgtgcgcg
    10561 tcttccgccg cgcgaaaatc gcgcccgtag aaaagcgact cgatgcgttg caaaaactcc
    10621 ggactcgtcg ccatcacatc cacgaactcg ccgtgcttga cgcaggtttt tttcatcagg
    10681 atgcgcccgt cttcttcgac gatctgcgct ttgatttcgc ccggatgctc gtgcatcaaa
    10741 gtctccagag gcgtctggcc ggagatgacg gcctcgcgca cttccttgac gcaggttggg
    10801 cacaaactgt ccgtctcacg cgggaagccg agcggcggcg cggtcttctc gtaagatttc
    10861 aggagcggcc ccggcgccca ctgcgggcga atcgcttcgc cttccggcag cgcacgattc
    10921 acggactgaa acaccttcca cgccgcattc gataagttcg aaataagcgt cgagcttttc
    10981 accccgctga atttgcttct caccgattcg ttacggcgaa attgatcgct aaacactaga
    11041 cgttcatcct ccgggagtta attgcacgcg tcaactttga tgcttgaagt tttaggaaag
    11101 tcgccggact cttaacgcgt ggctcatgca agaccatcgc ccgcgccgat tcgcgttgag
    11161 acgcaggcca accgccagac gacgcgctat gataaaggca tccgcccgta aagacaagac
    11221 agcagaagca cttggcgacg ctgcgccctg ttgtttggtt tcgctcagcg cactttaacg
    11281 aggaacacgt cccacgccca acgtgtaact ctcgcgccct gcgcatcatt caacttcgca
    11341 cgatagcgaa actcgttgcc ctgcccgtcc acccgcttcg actccttaag tacgacaggc
    11401 gcaggctcaa cacactcaac gacggcaaat tgtgcaactt cgtggcctct gagatgccgt
    11461 tgtgtttggt gtcctgccac agccgcaaac gagagtagac ggggtcttgt tgatcaatca
    11521 gtccgtctcc gtctccattg ccgccgttag tcgtccaatc atagacggag agggcgcgga
    11581 agccgttgcg ctcttgccct gcgggcggcg ggggttgcgg cgtgtagttg ccgaagggtt
    11641 ctgctgcctg tcctgacctc ttgtgagtcc gggcagcggt tgatatttaa agagaccgcc
    11701 acgcgcgcct ctcgcatgac ggtctcgtgg catggccgct tcaccggact cggccacacc
    11761 ttcgggatgc ccacctcatc ccgaatccta aagaattatt aagaattatg cagacttttc
    11821 ggccagcagg cgcgccaccg cctcgaccag tcgcgccatg tcctgcggct tacgtagaaa
    11881 agcgtccacc cccgcacgcc gcgcattctc ctcacagtca ctcgccgaaa acatgatgat
    11941 gggcgtttgc ctgcgatgcg gcagaccccg cgcacgccgc acaagctcaa tgccgctcgc
    12001 gcccggtaac tcgttatcaa acaagagcag gtcgtaacgc gtctcccccg ccaactggtg
    12061 cagcgcgtcc accccgtcgg cgcaagtctc gaccgtccac ccctcctcac acagcgtctc
    12121 cctaaccgcc tccgcgagtt gcccgttatc ctcaacatgc agaatcacga ccgaccgcgc
    12181 cgctttctct cccgcctgct cccgcgcccg acacggatcg agcgcacgga taatgctccg
    12241 cttccgcggc acaataggcg tccgcacatg cagcaaatgc ttatgcctat tcttgagcag
    12301 cgacaccaga ctctgatgat gcttaacacc aagcaactgc gccgccctcg acaccatccc
    12361 gtccgcatcc ttcaacgcac gctcaatcaa atgcgactca taacggcgca ccgcatcctt
    12421 aaaagaaaac cccgtccagt catgcggtgt cggcgacaag tgagtcgaga acaacacccg
    12481 ccgcgcacac gcgcacagcc gcttcaggtt cgccatgttc tgcgacttgc ccaacaactc
    12541 atccgcgcgc acatatatct cactcaactc cgaccgggac aaatgcgacc cacactcctc
    12601 aataatcgtc atcgccgcct gccccgcact ctcaagatca cccgcctcct cagccacctt
    12661 gatcgctttt ttcagtgcct catacgcatt ccccgcgcga cccagccgcg ccaacgcaat
    12721 cccatgagtt atcaacgcct cgacgtacaa cgagtgctca tcaccctttt ccagcactcg
    12781 caccgccgaa ttcgccaact tctcagcttc cacctctcgg cgttgcttca acagaacttt
    12841 agcccgcgtc tcatccacct gcgccagatg aactctgtcc ttgagactcg taaacagtgc
    12901 ttgcgccctg tcaagatgct catgtgcttc cgcgaaccta ctgatagtgc cgtataaaaa
    12961 acctaaatta ttttcaacgc aagcctgata acgtgcgtgc ccaaccttct cgaaatagaa
    13021 gcccgccgcc gtatattcca tgagtgcgcg atctatataa tcctcgcgat attccgctgc
    13081 aacgagatag ttcaacacat tagcgaatcc attatg
//
LOCUS       DASWNS010000134         7835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_160621, whole genome shotgun sequence.
ACCESSION   DASWNS010000134 DASWNS010000000
VERSION     DASWNS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160621"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /gene="otsB"
                     /locus_tag="VGB76_04470"
     CDS             <1..581
                     /gene="otsB"
                     /locus_tag="VGB76_04470"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY0098190.1"
                     /translation="WFGAVSGLWLAAEHGAIMRSPETMRWELYHPTYTSDWKEQVFPV
                     LEHFMDRTPGSLIEEKEFSLVWHYRMADPEFGEWLANELVANLEQMLADTELRAYRGN
                     KIVEVKPGWANKGEVLARLTEATPQAGFCLAAGDDRTDEDLFARLSADAWTVHIGERR
                     TRAKYCLPNSDELRQLLTRFADGQRVRESHAG"
     gene            complement(606..1751)
                     /locus_tag="VGB76_04475"
     CDS             complement(606..1751)
                     /locus_tag="VGB76_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098191.1"
                     /translation="MNAVTPEQVREIIDRSYQLEEGDSKIMLLEEAVRLADICGDFSL
                     AYDARELLINAATFGGAPEKALVAYSWCLAQFDRRGAAEGIDEWLLLWMYKWILNNVT
                     SFPQISKAQIEEMLDDMAARYRRAGYSLRPNYMLRNRIYKFWDETKAGEFFRKSQETY
                     RDLASDCAACELDEEVSFALRQSRDAEAVSLAAPIIQGRLRCDSVPHITFANLLLPLL
                     RLGRMDEAVSLHLQGYRLIARGSKGYVSSVADHLAFLVLTENFDRAVRIFEKHLPDAS
                     SLADLAAKFDYYIASWLLLDALHEGGRETLKLRLPRPLEVYEESGAYRTTRLAAWFLQ
                     HAQTLAARFDARNESDFYARRIRETAALKQLARSHPIRETEEEYTGG"
     gene            complement(1754..3583)
                     /locus_tag="VGB76_04480"
     CDS             complement(1754..3583)
                     /locus_tag="VGB76_04480"
                     /inference="COORDINATES: protein motif:HMM:NF010683.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HSP90 family protein"
                     /protein_id="HEY0098192.1"
                     /translation="MVDYKFQINLGGIIDLLSNHIYSSPQIYVRELLQNGVDAILTRH
                     YVEPAHEGAIRIRVLDAPGGGQPSLVFEDNGIGLNEEEIHGFLATIGQTSKRGDLATQ
                     RTDYIGQFGIGLLSCFVVSDEIVVVTRSAKGAAARTMEWRGRADGTYSIRQLEEETEP
                     GTRVYLRCKAGSEEYFKPQAVRRMARHYGSLLPYPITLAAGDGAPLPVNEEQPPWKRT
                     FDDAQDERAAYLAYGRETFDLTFFDYVPLRSQVGDVQGVAFVLPFSPSLATKRTHKVY
                     LKNMLVSENAEGLLPGWAFFVKCVVNANELRPTASRESFYEDEALSLTRGALGRCLRA
                     YLVELAQEQPQRLQKLISLHFLSIKALAVDDEEFYRLFIDWLPFETNAGQLTLGEYKK
                     QNRVLRYVPDLDQFRQIARVAASQSLIVINAGYVHDADLLARHSELFPSTPVEAVDST
                     SLMHSFEFLTLDEQERTADFTRFADRVLRPFNCATEMRKFSPRELPALYNASSEVNFH
                     RSIEQAREISAPLWSNVLGNLTKELPGDAFASLCFNYDNPLVYKLSRMRDETLLQLSI
                     QMLYVQSLLLSHRPLNAKEMSLLNDGLLGLLEWGVEVEGGWVQ"
     gene            complement(3627..4268)
                     /locus_tag="VGB76_04485"
     CDS             complement(3627..4268)
                     /locus_tag="VGB76_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY0098193.1"
                     /translation="MNSLYHEQLYRTPALMARLGEFPLAVCGAGALGANIAESLARTG
                     FKRLRVVDRDRIEERNLSTQPFYRSEAGAFKARMLANALYRALGVEVDARSVELTGAN
                     ASKLLDGSALVVDAFDNSASRRVLKEYSATAGVPCLHAGLVGDYAEVIWNEVYRVPSA
                     AQDDVCDYPLARNLVTLTVAVACESIVEFVTTGARRSYTITLRDFAVRPYRPD"
     gene            complement(4321..4779)
                     /locus_tag="VGB76_04490"
     CDS             complement(4321..4779)
                     /locus_tag="VGB76_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098194.1"
                     /translation="MSILEDERGGASLAERACEVERLVREFEACTLPGERWTHEAHLT
                     VALWYLTCHTDAEATRLIREGIQRYNSSRGVEMTKDGGYHETITLFYIRAIRRYLDGA
                     GETQTLAELSGGLTAACGGKNFPFKYYRRERLLSWEARMRWLEPDLRALD"
     gene            complement(4791..5657)
                     /locus_tag="VGB76_04495"
     CDS             complement(4791..5657)
                     /locus_tag="VGB76_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098195.1"
                     /translation="MDIRRLVQRLAAREDSLRGERFLAPCVGGGQVRVSLDGLVCAFR
                     PRPRGFEGWGIFQPVDETTAELFEEATLAQVAGYLRLLRPLRVRLAERLRGRSWLAYP
                     VNVEDVRVQPGACVELCVHLVSDGARFEGAVAYTDGCNWFYGEADRRADACIAERLRK
                     LLRQGVEPHDINLKGITPEMRAVYALATEFAPEFGHVRRRREAERAKQREEKRLRDAL
                     GLNGGALVNYEDDDENWRVVWRTRGGEEHVSTVAKRDLTVVSAGICLSGYDRDFDLQS
                     LVGVVEKGWDYD"
     gene            complement(5805..7238)
                     /locus_tag="VGB76_04500"
     CDS             complement(5805..7238)
                     /locus_tag="VGB76_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098196.1"
                     /translation="MENLERDMRLEMLNSLLTTPHRRLEEVSELHRRMLERDPLFYGH
                     LAVWYAEHGDVRDHKEVFTGNLLMSTVAGHRSAGFVLLQNLPPYQVARVVDFVKRARG
                     TLPRSARTAVVRYLREREENARHFDAAALRARKSMKHLYAGLHIKPSLRADAILFKDA
                     PPADSLAYAVKRLAQAATAAAQAELIVEHKIPYTVAVGAVRKLTPAVLAALINSMSPQ
                     ETINNLKSLKARGALAHAEAKALVEEKLAGAATGERVSAFKAVRAAEVAGVDAQTTAR
                     LEHAASEQLRKRGRITKTTALFVDKSGSMTLALEVGKRIAALVSGVAEAKLYVYAFDT
                     IGYPVVSAGGGELSDWERAFKHLHAQGGTSIGAPLETMRLRRQAVEQIIIVTDEGENT
                     APYFNAVYARYCAELKVAPNVVIVKVGQHTDYLERQMSQSKVSFETFTFAGDYYALPN
                     LVPLLSRPSRLELLMEILETPLPERKV"
     gene            complement(7456..7527)
                     /locus_tag="VGB76_04505"
     tRNA            complement(7456..7527)
                     /locus_tag="VGB76_04505"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7493..7495),aa:Phe,seq:gaa)
BASE COUNT         1495 a         2478 c         2372 g         1490 t
ORIGIN      
        1 attggttcgg cgcagtttcc gggttatggc tcgcggcgga gcacggcgcg atcatgcgct
       61 cgccggagac gatgcgttgg gaactttatc acccgaccta cacgagcgat tggaaggagc
      121 aggtcttccc cgtgctcgaa catttcatgg accgcacgcc cggcagtctt atcgaagaga
      181 aagagttttc gctcgtctgg cattaccgta tggccgaccc ggagtttggc gaatggctcg
      241 ccaacgaact cgtcgccaat ctcgaacaga tgctcgccga cacggaactg cgcgcctatc
      301 gcggcaacaa gatcgtggaa gtgaaaccgg gatgggcgaa caagggcgag gtgctcgcgc
      361 gactcaccga agcaaccccc caagccgggt tctgtctcgc cgcaggcgac gaccgcaccg
      421 acgaagatct cttcgcgcgt ctctcggcag acgcctggac agtccacatc ggcgaacgcc
      481 gcacgcgcgc caaatattgt ctaccgaact ccgacgagtt gcgccaattg ctcacgcgct
      541 tcgccgacgg ccaacgcgtg cgcgagagcc acgccggatg aaccgtttgc ggcaggcgga
      601 cgcgctcacc cgcccgtgta ctcttcctcc gtttctctga tcgggtggct gcgtgccagt
      661 tgcttgagcg cggcggtttc gcggatgcgg cgtgcgtaaa agtcgctttc gttgcgcgcg
      721 tcgaagcgtg cggcgagagt ttgcgcgtgt tgcagaaacc atgcggcgag gcgtgttgtc
      781 cggtacgcgc cgctctcttc ataaacttcg aggggacgcg gcaagcgcag ttttaaagtt
      841 tcgcgcccgc cttcgtgcag ggcgtcgagc aggagccacg aagcgatgta gtagtcgaac
      901 tttgcagcga ggtcggcgag cgaggaagcg tccgggaggt gcttctcgaa gattctcacg
      961 gcgcggtcga agttctcagt caggacgaga aaggcgagat ggtcggcgac gctggagacg
     1021 tagcccttgc tcccgcgtgc gatgagacga tagccctgca agtgcaggct cacggcctcg
     1081 tccatgcgac cgagacgcag cagcggcagc aacagattcg caaaagtgat atgcggaact
     1141 gaatcgcagc gcaggcggcc ttgaatgatc ggcgcggcga gactgacggc ctccgcgtct
     1201 ctgctttgac gcagcgcgaa ggaaacctct tcgtcgagtt cgcaggcggc gcaatcgctc
     1261 gcaaggtcgc ggtatgtctc ctgcgacttg cggaaaaact ccccggcctt cgtctcatcc
     1321 cagaacttat agatgcgatt gcggagcatg tagttcgggc gcaaagaata acctgcgcgc
     1381 cggtaacgcg cggccatgtc gtcgagcatc tcctcgatct gcgccttgct gatctgcggg
     1441 aagctcgtga cgttgttcag aatccacttg tacatccaca gcagcagcca ttcgtctatg
     1501 ccctccgctg cgccgcgccg gtcgaactgc gcgagacacc acgagtaggc gacgagagcc
     1561 ttctccggcg cgccgccgaa ggtcgcggcg ttgatgagaa gctcgcgcgc atcgtaagcg
     1621 agtgagaagt cgccgcagat gtctgcgagg cgcacggcct cttcgagcag catgatcttc
     1681 gagtcgccct cttccagttg ataggagcgg tcgatgatct ctctgacctg ttcgggcgtt
     1741 acggcgttca tcgttattgc acccagcccc cttccacttc cacgccccat tcgagcaggc
     1801 cgagcagccc gtcgttcaac agcgacatct ctttcgcgtt gagcgggcga tggctgagca
     1861 ggagcgactg cacgtagagc atttgaatcg aaagctgcaa gagcgtctcg tcgcgcatcc
     1921 ggctcaactt gtagacgagc gggttgtcgt agttgaagca cagagaagcg aacgcatcgc
     1981 ccggcaactc cttcgtgaga ttgccgagca cgttcgacca gagcggcgcg gagatctcgc
     2041 gcgcctgttc gatggaacga tgaaagttga cttcgctcga cgcgttgtag agcgcgggca
     2101 gttcgcgcgg cgaaaacttg cgcatctccg tggcgcagtt gaacgggcgc agcacgcgat
     2161 ccgcgaagcg tgtgaaatca gcggtgcgct cctgctcgtc gagcgtcaaa aattcgaagc
     2221 tgtgcatgag gcttgtggag tcaaccgcct cgacgggcgt ggacgggaag agttcggagt
     2281 ggcgtgcgag caagtccgcg tcgtggacgt atccggcgtt gatcacgatg agcgattgcg
     2341 aagcggcgac gcgcgcgatc tgccggaact ggtcgagatc gggcacgtag cgcagcacgc
     2401 ggttctgctt cttatattcg ccgagcgtca actgccccgc gttcgtctca aacggcagcc
     2461 agtcgatgaa gagccggtaa aactcttcgt cgtccacggc gagcgccttg atcgagagga
     2521 agtggagcga gatcaatttt tgcaggcgtt gcggctgctc ctgcgccagt tcgacgaggt
     2581 aggcgcgcag gcagcgaccg agcgcgccgc gcgttaagct caacgcttcg tcttcgtaga
     2641 aactctcgcg cgaagcggtc gggcgcaatt cgttcgcgtt gacgacgcac ttgacgaaga
     2701 aagcccaacc gggcagcaag ccttccgcgt tctccgagac gagcatgttt ttcaggtaga
     2761 ccttgtgcgt ccttttcgtg gcgaggctcg gcgaaaacgg cagcacgaag gcgacgccct
     2821 gcacgtcgcc cacctgcgag cgcagcggaa cgtaatcgaa gaacgtgagg tcgaaagtct
     2881 cgcgcccgta ggcgagatag gcggcgcgct catcctgcgc gtcgtcgaaa gttcgcttcc
     2941 agggcggctg ctcttcgttg acgggcaggg gcgcgccgtc gcccgccgcg agcgtgatcg
     3001 ggtaaggcag caggctcccg taatgacgcg ccatgcggcg caccgcctgc ggcttgaagt
     3061 actcttcgct gcccgccttg cagcgcaggt acacgcgcgt gcccggctcc gtctcttctt
     3121 cgagttgcct gatggaataa gtgccgtcgg cacgaccgcg ccactccatc gtgcgcgccg
     3181 ctgcgccttt cgccgaacgc gtgacgacga cgatctcgtc gctcacgacg aagcaggaga
     3241 ggaggccgat gccgaactgc cctatgtaat cggtgcgctg cgttgccaga tcgccgcgct
     3301 tcgaggtctg gccgatggtg gcgagaaagc cgtgtatctc ctcttcgttt aaaccgatgc
     3361 cgttatcttc aaagaccagc gagggctggc cgccgcccgg cgcatcgagc acgcggatgc
     3421 ggatcgcgcc ctcgtgtgca ggctctacgt agtggcgcgt gaggatggcg tccacaccgt
     3481 tttggagcag ttcgcggacg tagatttgcg ggctgctgta gatgtggttc gagagcagat
     3541 caatgatgcc gcccagattg atttgaaact tgtagtccac cattcacctc tcgaaaattc
     3601 ccgcccgcta attttaagca agtccgctag tcaggccggt aaggtctgac ggcgaaatcg
     3661 cgtagcgtga tcgtgtaact ccggcgcgct cccgtcgtga cgaactcgac gattgactcg
     3721 caagcgacgg cgaccgtgag cgtgacgaga ttgcgcgcca gcgggtagtc gcaaacgtca
     3781 tcctgcgccg cagagggcac gcgataaact tcgttccaga tcacttccgc gtagtcgccc
     3841 acgagtcccg cgtgcaggca gggcacgccc gccgtcgcag aatattcctt caatacgcgc
     3901 cgcgatgcgc tgttgtcaaa cgcgtcaacg acgagcgcgc tcccgtccag caacttcgac
     3961 gcgttcgccc ccgtcaactc gacgctccgc gcatccacct cgacgccgag agcgcgatac
     4021 agagcgttcg ccagcatccg cgccttgaac gcgcccgcct ccgagcgata gaaaggctgg
     4081 gttgagagat tgcgttcctc gatgcggtcg cggtcaacga cgcgcaaccg cttgaacccc
     4141 gtgcgcgcga ggctctccgc gatgttcgcg ccgagcgcgc ccgccccgca caccgccaga
     4201 ggaaactcgc cgaggcgcgc catcaacgcg ggcgtgcgat aaagttgttc gtgatagaga
     4261 ctgttcatcg caaaaattcc acacgcgttc cggcgcgcat tcttcgtgcg cgcgtcgcgt
     4321 tcaatcgagc gctctcaggt cgggttcgag ccaacgcatg cgcgcttccc aagagagcag
     4381 acgctcgcgt ctgtagtatt tgaacggaaa gtttttcccg ccgcacgccg ccgtcaaacc
     4441 gccggacagt tcggcgagag tttgcgtctc gcccgcgccg tcgagatagc ggcgaattgc
     4501 gcgtatgtaa aagagcgtga tggtttcatg gtagccgccg tccttcgtca tctccacgcc
     4561 gcgcgacgaa ttgtagcgtt gaattccttc gcgtatgagc cgcgtcgctt ccgcgtccgt
     4621 atggcacgtg agataccaga gcgcgaccgt caaatgcgcc tcgtgcgtcc aacgctcccc
     4681 cggcagcgtg caggcttcaa attcacgcac cagacgctcc acttcacacg cgcgctctgc
     4741 caacgacgcc ccgccgcgtt cgtcttccaa gatagacatc gccgccctct ctaatcgtag
     4801 tcccaacctt tttccaccac gccgacgagc gattgcaggt cgaagtcgcg gtcgtaaccg
     4861 ctcaggcaga tgcccgcgct gacgaccgtg agatcgcgtt tggcgacggt cgaaacgtgc
     4921 tcctcgccgc cgcgcgtccg ccagacgacg cgccagttct catcatcgtc ttcataattg
     4981 acgagtgcgc cgccgtttaa gccgagcgcg tcgcgcagac gcttctcttc gcgttgcttc
     5041 gcgcgctcgg cctcgcgtcg cctgcgcacg tggccgaact ccggcgcaaa ctccgtggca
     5101 agcgcataga cggcgcgcat ctccggcgtg atgcccttca agttgatgtc gtgcggctcc
     5161 acgccctgcc gcagaagttt ccgcaagcgt tcggcgatgc acgcatcggc gcgccggtcg
     5221 gcttcgccgt agaaccagtt gcagccatcc gtgtaagcga ccgccccctc gaagcgtgcg
     5281 ccatccgaga cgagatgcac acacagttcg acgcacgcgc ccggctgcac acgtacgtct
     5341 tcaacattca ccggatacgc gagccacgac cgaccgcgca gacgttcggc cagacgcacg
     5401 cggagcggtc gcaacaatct caaataacct gcgacctgcg ccagcgtcgc ctcttcgaag
     5461 agttccgccg tcgtctcgtc aaccggctgg aagatgcccc acccctcgaa gccgcgcggg
     5521 cgcgggcgaa aggcgcacac gagtccgtcg agcgacacgc gcacctgccc gccgccgacg
     5581 cacggcgcga gaaatcgctc gccgcgcaga gagtcttccc gcgcggcgag ccgttggacg
     5641 agtcggcgaa tatccacaag accaaatgat gaaggcggaa cgaggaatga aaagcaggag
     5701 acagaattca gaagtcagaa gtcagaataa agcaggcgcg tctcttgctt ctgactcctg
     5761 tattctgttt tctgaattcc ggttcatcct tcgtcgttcc gccttcatac tttcctttcc
     5821 gggagcggcg tctccagaat ctccatgagc aactcaagcc gcgagggacg gctgagcaac
     5881 ggcacgaggt tcgggagcgc gtagtagtcg cccgcgaagg tgaaggtttc aaacgacacc
     5941 ttgctctggc tcatctgccg ctcaaggtaa tctgtatgct gaccgacctt gacgatcacc
     6001 acgttcggcg caaccttcaa ctccgcgcag taacgcgcat aaacggcgtt gaagtaaggc
     6061 gcggtgttct cgccttcgtc cgtcacgatg atgatctgtt cgaccgcctg acggcgcagg
     6121 cgcatcgtct cgagcggcgc gccgatgctt gtgccgccct gcgcgtggag gtgcttgaaa
     6181 gcgcgctccc aatcgctcaa ctcgccacct cccgcgctca cgaccgggta accaatcgtg
     6241 tcgaacgcat agacgtagag cttagcttcg gccacgccgg agacgagcgc ggcgatccgc
     6301 ttgccgactt cgagcgcgag cgtcatgctc ccgctcttat caacgaacaa cgccgtcgtc
     6361 ttcgtgatgc gcccgcgctt gcgcaactgc tcgctcgcag cgtgctcaag ccgcgccgtc
     6421 gtctgcgcgt caacgcccgc gacctccgcc gcacgcacgg ccttgaacgc cgagacgcgt
     6481 tcgcccgtcg cagcccccgc gagtttctct tcgacgagag ccttggcctc cgcgtgcgcc
     6541 agcgcgccgc gcgccttcag agacttcaag ttgttgatcg tctcctgcgg cgacatcgag
     6601 ttgatgagcg cggcgaggac ggcgggcgtc aacttccgca ccgcgccgac ggcgaccgtg
     6661 tagggaatct tgtgctcgac gatcaactcc gcctgcgctg ccgccgtcgc ggcctgcgcc
     6721 agacgcttca cagcgtaggc gaggctgtct gccggaggcg cgtccttgaa gaggatcgcg
     6781 tcggcgcgca gagacggctt gatgtgtaag cccgcgtaga gatgcttcat gctcttgcgc
     6841 gcacgcagag ccgcggcgtc gaaatggcgc gcgttctctt cgcgctcgcg caggtaacgc
     6901 acgaccgccg tgcgcgccga acgcggcaga gtgccgcgcg cacgcttcac gaagtcaacc
     6961 acgcgcgcca cctgataagg cggcaggttc tgcaacaaca cgaagcccgc gctgcgatga
     7021 cccgccaccg tgctcatcag caggttgccc gtgaacacct ccttgtgatc gcgcacgtcg
     7081 ccgtgctccg cgtaccacac cgccagatga ccgtagaaga gcgggtcgcg ctcaagcatc
     7141 cggcgatgca actccgacac ctcttcgagg cgacggtgcg gcgtcgtcag caaactattc
     7201 aacatctcca ggcgcatgtc gcgctcaagg ttctccatga cacacctctc tcagacagtg
     7261 acaagtgaca ggtgacgggt gacgggtgac aagttaaaga caggtgaatc gtgaatcgtc
     7321 aatcgtgaat agatgaaacc aggtcttact atctattgac gattcacgat tgacgattca
     7381 cggctttttc tcgtcacctg tcacccgtca cctgtcactg tctttattcc cccgcgcaag
     7441 tcagggaagc gtctttcaca taagcgggaa tcgaacccgc gacactacgt tcttcaaacg
     7501 tatgctctac cactgagcta gtatgtatgc ttcgccggtc agggcgcggg gtgaaaacgt
     7561 ctggtaaaac gtgaatcgta aagagagaaa agctgtctgc ttcgattcac gattcaaagc
     7621 ttttgaatct atcggcgatt taagattttt cggtcggcct ccgtgccgga gcgaggctgc
     7681 gcgcaagtct gtgaagcgtt tcttgaccca ccttgtgaag ggcgggctgc acgctcccat
     7741 aagttatgcg ggagcggtag gattcgaacc tactgcggtt ttggtgaacc gttctaccat
     7801 gtatgtatgc ttcgcaggtt ggggcgcgca gccgc
//
LOCUS       DASWNS010000135         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_264702, whole genome shotgun sequence.
ACCESSION   DASWNS010000135 DASWNS010000000
VERSION     DASWNS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264702"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1911
                     /locus_tag="VGB76_04510"
     CDS             <1..>1911
                     /locus_tag="VGB76_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY0098197.1"
                     /translation="EYFAEGQAHPDKIDARQAIYFVETEAAVFNGNVVIETRDRLIAK
                     AETINYDVKNEHAEVPVAVTFVRENVEGRSDAASVDAKNKKLELRGNVEITVKPDANQ
                     ATAQTPGGASGTRNRPVTIRAPRANFDHANLHLLFSGGATAVQEQDVMSGETLAGFLN
                     PEKRIQRIEARTNAYLRTMDAGRAAEVSSVNMDFFFDNNQQLQQAVAAQNVRARSLDA
                     ESQVELSAPHDLQVFFEAAGEQSLLKEMRAEGRSVVTLAAPRSRANDPQAANKKLTAN
                     TVRLFWRKTGKDLERAEAVGDAELIVEPVQQTALADRKTLNAPRFDCNFFEAGNLARD
                     FNATGGAKAVLTPVAPNSARSVRTITSQKMFAFFTRETQDVERLDAQGETQFNDADRN
                     GQAETASYTAADEVVRLRGGAPVVWDSRARLKASEIDSDTRKKISYARGKAQTTYYSQ
                     EQTNGATPFSKVKSPVFLASNAVEFQHDSGIGIYTGDARAWQDDNFVSADKLTLRRDN
                     RRMEGDGRVQSALYQARRKEASGARTVVPVFATSNRMFYSDSDRLLHYEGNVDIKQGT
                     ERITSEVADVYLMKELGEVERMVAQRNVVLTQPGKRGTGNWAQYSAADETVMLTGEPA
                     RVEDPQQGNSESR"
BASE COUNT          413 a          603 c          617 g          278 t
ORIGIN      
        1 cgaatatttc gcggagggac aggcgcaccc cgacaagatc gacgcgcgac aggcgatcta
       61 tttcgtcgag acggaggccg ccgtcttcaa cggcaacgtc gtcatcgaaa cgcgcgaccg
      121 cctgatcgcc aaagcggaga cgatcaacta cgacgtgaag aacgagcacg ccgaagtgcc
      181 tgtcgccgtt acgttcgtgc gcgagaatgt cgaagggcgt tcggatgctg cgtcggttga
      241 cgcgaagaac aaaaagcttg agttgcgcgg aaacgtcgag atcacagtca agcccgacgc
      301 caatcaggcc accgcgcaga cgccgggagg cgcgagcggc acacgcaatc gtcccgtcac
      361 gattcgcgcg ccgcgcgcca atttcgacca tgcgaacctg cacctcctct tcagcggcgg
      421 cgcgaccgcc gtgcaggagc aggacgtgat gagcggcgag acgctcgccg gattcctcaa
      481 cccggagaag cgtatccagc gcatcgaagc gcgcacgaac gcttacctgc gcacgatgga
      541 cgcgggacgc gcggcggaag tcagttccgt caacatggat ttcttcttcg acaacaacca
      601 gcaactacaa caagcagtcg ctgcgcaaaa cgtgcgcgcc cgttcgctcg acgccgaatc
      661 gcaggtggaa ctcagcgcgc cacacgatct gcaagtcttc ttcgaagcgg cgggcgaaca
      721 gagtctgctc aaggagatgc gcgccgaggg tcgttccgtc gtcacgctcg ccgcgccgcg
      781 ctcgcgcgca aacgatccgc aggccgcgaa taaaaagttg accgccaaca ccgtccgtct
      841 cttctggcgc aagacgggga aagacctcga acgcgcggag gccgtgggcg atgccgaact
      901 gattgtcgaa cccgtgcagc agaccgcgct ggccgaccgc aagacgctca acgccccgcg
      961 cttcgattgc aacttcttcg aggccggaaa tctggcgcgc gacttcaacg ccaccggcgg
     1021 cgcaaaggcc gtgctgactc ccgttgcgcc gaactccgcg cgctccgtgc gcacgatcac
     1081 gtcgcagaag atgttcgctt ttttcacgcg ggagacgcag gacgtggagc gtctggacgc
     1141 gcagggcgag acgcagttta acgacgcaga ccgcaacggg caggccgaga cggcgagcta
     1201 tacggctgcc gacgaagtgg tgcgactgcg cggcggcgcg cccgtcgtgt gggattcgcg
     1261 cgcgcgcctg aaagcctcgg agatcgattc ggacacgcgc aagaaaatct cttacgcgcg
     1321 cggcaaagcg cagacgacct attacagtca ggaacagacg aacggcgcga ccccgttttc
     1381 gaaagtcaag agtcccgtct tcctcgcttc aaacgcggtc gagtttcaac acgattccgg
     1441 catcggcatc tacacgggcg acgcgcgcgc gtggcaggac gataactttg tgagcgcgga
     1501 caaactcacg ctccggcgcg ataaccggcg catggagggc gacgggcgcg tgcagagcgc
     1561 gctctatcag gcgcggcgca aagaggcttc gggcgcgcgc acggtcgtgc ccgtcttcgc
     1621 cacttcaaac cggatgttct actctgactc ggatcggctg ctgcactacg aaggcaacgt
     1681 ggacatcaag cagggcacgg agcgcatcac gagcgaagtg gccgacgtgt atttgatgaa
     1741 agagttgggc gaggtcgagc gcatggtggc gcagcgcaac gtcgtcctca cgcagccggg
     1801 caaacgcggc acgggcaact gggcgcagta cagcgcggcg gatgagacgg tgatgttgac
     1861 gggtgaaccc gcccgcgtcg aagacccgca acagggcaac agcgagagcc g
//
LOCUS       DASWNS010000136         4569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_473943, whole genome shotgun sequence.
ACCESSION   DASWNS010000136 DASWNS010000000
VERSION     DASWNS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473943"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..2185)
                     /locus_tag="VGB76_04515"
     CDS             complement(29..2185)
                     /locus_tag="VGB76_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0098198.1"
                     /translation="MALRRNTVLLPGGILTALLISLTILINVHASGGQDHSAPVVIMV
                     PDEQTVDDISPEQRAEAEKKIAKHERTKKAKKKSEGITTMAPSDPPTLQPYAFFPQAG
                     TLWQDLYVNNFVDLDPAGGILDWDCTQQTYNGHTGHDSDIRTFREQGIGVPVFAALDG
                     TVVDLHDGEADMNTSAQGQAANYVILDHGNSHYTWYWHLKNGSVAVTMNQVVKAGTQL
                     GLTGSSGSSTHPHLHFESRYGGAAFEPSAGPCRTGTSNWVQQVPIRRDMYVRDFGFST
                     TALSGNLAFDNSTRTGTYLLGTSGEWFRTDLSNVPANSSYRINYRRPDGTIALDYVGN
                     FNNSAPYRKATFYWNFLVNLNVTGTWRLQMTVNNQALIDTPFQVVSSQSQIVNRAPNS
                     ITLGLDPAAPSASDVIFCRVQTSLILEDPDYDTVRYRYRWSVNGTVVRDVTSAALSDA
                     LRKGLTNGGDSVTCNVTPSDGLLSGAAASSVVNISGSPTGSVLQNGVPVSGLSGAGGS
                     QQFWTMSVPAGASNLQFQTSGGTGDADLYVRFGSAPTTSTYDCGPVTGDNNELCSFAS
                     PGAGTWHVMVHGYAPYSGATLVGSYQTGSSSTCNSVSDAEPNNSMAAPQVISGACNQV
                     SGTFLNDASSQQNDFFRLNLPAGQTVTALLNGLTVDYDLYIYDAAGNQVASSEAGGAS
                     ADQASWTNGGASAVNVYVLIYRYASTQTTYQLKVSY"
     gene            2344..3057
                     /locus_tag="VGB76_04520"
     CDS             2344..3057
                     /locus_tag="VGB76_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012168287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEY0098199.1"
                     /translation="MKRSASPFKWRHYAPDVILLCVRWYCRYSLSYRDLEEMMRERGL
                     AVDHVTIFRWVQRYAPEINKRMRPHLKMSGTSYRLDETYVKVSKEWKYLYRAVDSAGQ
                     TIEFMLSAKRDVSAAKRFFRKVMRADHRRLPFTIGTDKHASYPEAFATSVKEKILPAD
                     CKLRRVKYLNNVIEQDHRAIRRRWRAMQCFRSFHTAERTLEGVEALHMMGKGQVKRLD
                     GRDLVGQAKFVESLFGVAA"
     gene            complement(3328..3975)
                     /locus_tag="VGB76_04525"
     CDS             complement(3328..3975)
                     /locus_tag="VGB76_04525"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0098200.1"
                     /translation="MAKDKASIPGLIEQVMRHPNEVFSKDREFEAASRLAKSGAPAVL
                     PILKAMQGPYPPGQHPVDVVEALGCVLKGIARRESAPLVEVLEGGAFPPDAGLSYVVY
                     ALRDANDERALRALISALKHPHKYIRDAAASVLVEKDDGRVVVPLVEALSDKSSSVKF
                     TVVQAMRRRTDLRDTRALEPLKRIVASKSLQKHSPGMCEYASEVIGMIEGRGGPS"
BASE COUNT          868 a         1416 c         1390 g          895 t
ORIGIN      
        1 ccccgtcgga ggggcgggcg cttccacgtc agtagctgac cttaagctga taggtcgttt
       61 gcgtcgatgc gtagcggtag atgaggacgt agacgttgac cgcagaggcg cccccgttgg
      121 tccaggacgc ttggtccgcg ctcgcgccgc cggcctccga actcgccacc tgattcccgg
      181 ccgcatcgta gatgtagagg tcatagtcca ccgtcaggcc gttaagcaac gccgtcaccg
      241 tctggcctgc cggcaggttc agccggaaga aatcattctg ctgcgacgac gcgtcgttga
      301 ggaacgtccc cgagacctga ttgcaggccc cagagatcac ttgcggcgcg gccatgctat
      361 tgttcggctc cgcgtccgag acggaattgc aggtagacga cgagcccgtc tggtagctcc
      421 cgacgagcgt cgcgcccgaa tacggggcgt agccgtgcac catcacatgc caggtgcccg
      481 cccccggcga cgcgaaggag cagagctcgt tgttgtcgcc cgtcaccggc ccgcagtcgt
      541 aggtggaggt ggtcggcgcc gagccgaatc gcacgtacag gtcggcgtcg ccggtgccgc
      601 ccgaggtctg gaactgaagg ttcgacgccc ccgcgggcac gctcatcgtc cagaactgct
      661 ggctgccgcc cgctcccgag agccccgaca ccgggacgcc gttctgcagc acgctgcctg
      721 tgggcgaccc cgagatgttc accacagagg atgctgcggc gcccgataag agaccgtctg
      781 agggagtcac attgcaagta acactatcgc cgccgttagt cagccctttc cgcagcgcgt
      841 ccgaaagggc ggcactagtt acgtcgcgga cgaccgtacc gttaacactc caccgatagc
      901 ggtaccgaac ggtgtcgtaa tccgggtctt ctaaaatcag tgaagtctgc acgcggcaga
      961 agatgacgtc ggaagcgctg ggcgccgccg ggtcgaggcc gagcgtgatc gagttcggcg
     1021 cgcggttaac gatctgcgac tgcgacgaga ccacttggaa cggcgtgtcg atcagcgcct
     1081 gattgttgac ggtcatctgc aggcgccacg tcccggtcac attgagattc actaaaaagt
     1141 tccagtagaa cgtggccttg cggtagggcg cggagttgtt gaaattgccc acgtaatcga
     1201 gggcgatggt tccgtccggg cgacggtagt tgatccggta actgctgttg gccggaacat
     1261 tcgacaggtc ggttctgaac cattcgccgg aggtgcccag caggtaggtg ccggtcctcg
     1321 tcgaattgtc aaaagcaaga ttgcccgaca gcgcggtggt gctgaagccg aagtccctga
     1381 catacatgtc acggcggatg ggtacttgtt gaacccagtt gctggtgccc gtccggcacg
     1441 gcccggcgga aggctcgaag gccgcgccgc cgtacctcga ctcgaagtgt aggtgcggat
     1501 gggtgctgct gccgctgctc ccggtcaggc cgagttgtgt gccggccttg accacctgat
     1561 tcatcgtcac ggccacgctg ccgttcttca ggtgccagta ccaggtgtag tgtgagttgc
     1621 cgtgatcaag aatcacgtaa ttggccgcct gcccctgcgc ggaggtgttc atgtccgcct
     1681 cgccgtcgtg caggtcgacg accgtcccgt ccagcgccgc gaaaaccggg acccctatcc
     1741 cctgctcccg aaaggtgcgg atgtcgctgt catgtcccgt gtgcccgttg taagtctgct
     1801 gagtgcagtc ccagtcgagg atgccgccgg ccggatcgag gtcgacgaag ttgttgacgt
     1861 acaggtcctg ccagagcgtg ccggcctgcg ggaagaaggc gtaaggctgc aaagtcggcg
     1921 ggtcagaagg ggccatagta gttatcccct cggacttctt cttggccttc ttagtgcgct
     1981 cgtgtttagc aatcttcttc tccgcctccg cgcgttgctc aggcgagatg tcgtcaacgg
     2041 tctgttcgtc aggcaccatg atgacgacgg gagcagaatg atcctgaccg ccggaggcat
     2101 gtacgttgat tagaatcgtt agcgatatta gcagcgcggt gagaatcccg ccgggcagta
     2161 aaaccgtgtt gcggcgcagc gccatcggat tccgccgtga gtgacaccga atagacatta
     2221 cggacctccc cctgggtgaa gaagtggcgg ggaacgagct cataaacggt tctgttgcaa
     2281 gaatcgcctt ccggttataa tccgtctctc acgttcttct agcaagcctt atcaggtcac
     2341 tacatgaagc gttcagcctc gccgttcaag tggcgccact acgcgcctga cgtcatactc
     2401 ctgtgcgtcc gttggtattg ccgctactca ctctcttacc gtgatctcga agagatgatg
     2461 cgcgagcgtg ggctggccgt ggatcacgtg acgattttca ggtgggtaca gcgctacgct
     2521 cctgaaatca ataagaggat gcgcccgcac ctgaagatga gcggcacctc ctaccggctc
     2581 gacgagacct acgtgaaggt cagtaaagaa tggaaatatt tgtaccgcgc ggtggactcc
     2641 gcggggcaaa ctatcgagtt tatgcttagc gccaaacgcg atgtttccgc ggcgaagcgg
     2701 ttctttagga aggtaatgag ggctgaccac aggaggctcc cgttcaccat cggcaccgac
     2761 aaacacgcct cctaccctga ggccttcgcg acttcagtga aagagaagat actacctgcc
     2821 gactgcaaac ttaggcgcgt caagtacctt aacaatgtga ttgaacaaga ccaccgagcg
     2881 atccgcagga ggtggcgagc tatgcaatgc ttccgctcct tccacacggc cgagcggaca
     2941 ctcgaaggcg ttgaagcact ccacatgatg ggtaaggggc aggtcaagag attagacggt
     3001 agagatttgg tggggcaggc gaagttcgtc gagagcttgt tcggagttgc cgcctagcag
     3061 tgtggggcct cacggcctca tttgcctcaa atcgatcttt gcaacagaac cgccccgcgg
     3121 agaaggataa tatccggcgt gtccgcggtc gggtgcatgc cgttgttaga ccggctcagc
     3181 gcgccgggct gaaacctccc gcgttcgcag cccggcgctt tcagccgatc taacgcccgg
     3241 catcacccgc cgccacgagc gactattgga agatgacagg cagcgtgtcg gcggtcgggt
     3301 gcatgccgtt gttgggcggc gccctggcta tgaagggccg ccccgaccct caatcatgcc
     3361 aatgacttca ctggcgtatt cacacatccc gggcgaatgc ttttgtaagc tcttactcgc
     3421 cacgattcgt ttcagcggtt cgagggcgcg ggtgtcgcgc aaatccgttc tacgccgcat
     3481 ggcctgtacg accgtgaact tgacggacga agacttatca ctcagcgcct cgaccaaagg
     3541 cacgacgacc cgcccgtcgt ccttctcaac taggacggag gccgcagcgt cccggatata
     3601 tttatgaggg tgcttcaacg ccgatatcaa agcgcgaagc gcgcgctcat cgttagcatc
     3661 ccttaacgca tagacgacgt aggagagccc ggcgtctggc gggaaagcgc ccccttcgag
     3721 cacctcgacc agtggcgccg actccctccg ggctatcccc ttcaagacgc agcctaaggc
     3781 ttcgaccacg tccacgggat gctggcccgg tgggtatggc ccctgcatcg ctttcaggat
     3841 tggcagaacc gcgggggcgc cggacttggc cagccgtgag gccgcctcga attccctatc
     3901 tttcgagaac acctcgttag ggtgtcgcat gacctgctcg attaatccgg gtatgctcgc
     3961 cttatccttt gccatcactt aacccctcat aaagcggagc cgcccaacgc cgcgcatcac
     4021 gcgcccgcca gccccttcga tgaaacatga aatcactcgt gtcgcgggtc gcgtgcatgc
     4081 gcttgttaga ttgcgggtca tctttagctc acttaccgcc gccggcccct gagctgggac
     4141 gggcggtctg cgcgatgccg gacgccaaag tcttgccccg ccgtcaggcc gtccattacg
     4201 gcctgcggcg cggcaaccag acagcttcgc cacccgccga cgccacgcct tcaatcagca
     4261 aagggctata tcgcaacagc aatctaacgc cgcgcatcac gtgcccgccc acccctcgac
     4321 ggagtaggag aggcagcgtg tcgcgggtcg cgtgcatgcg cttgttagac cgccccacag
     4381 cggcggcgcg aaaccttccg cgtatcgtgc tgccgcgttc aggcggttct aacgcccggc
     4441 atcacccgcc cgcccgcatg attgatttag atgagagccg ctgtgtcgcg ggtcgggtgc
     4501 atgccgttgt tatgctgcgc ctcgaccgca tcccagacct ctgcggccac atcctcggcc
     4561 cagtacccg
//
LOCUS       DASWNS010000137         5443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_473955, whole genome shotgun sequence.
ACCESSION   DASWNS010000137 DASWNS010000000
VERSION     DASWNS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473955"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3189
                     /locus_tag="VGB76_04530"
     CDS             <1..3189
                     /locus_tag="VGB76_04530"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEY0098201.1"
                     /translation="RRDEDERAVLLDSLGSLYATGHEVNWKGLFTDGGKCVHAPTYTW
                     QRERFWIEPAEPATDNGGVWRASRDGQRERPLLGAHVSPAHPSLEHLWEFRLSQTELP
                     FLTDHRVQGMMVLPATAYIEMALGAARQVFGGQAFRLTGLKFDKALFVPEAAALIVQL
                     GFSPPVEGVVGFHIYSRPEVSDGGRHLWTLHTTGQIHFNREEQEQEQANLPVEPLETI
                     QARCYNELSGDEFYRLLNDVGHDYGGSFKSIQTLWRRDAEAVAQFHISDDLAAEVSSY
                     QFHPAILDCCLQLLGATVAQNDELAARGAHVPVAIDEIRVKGRPAGQLWSHAALRVDE
                     ESPEKGSNNLKGDVSLRDEAGQIVIELVGVELQCLEEHSVRVAAGRVAEWMYEPVWEE
                     QPRVPQSLESRTARRADGAWLIFSDRQGIGEALANLLEEDGEKVLMVAPGEEFMRIDE
                     RNFLAPLTQAEDVREIYKAASGADGFGCRGIVHLWSLDSRGFSELTHETLRKESLDVC
                     ISVVNVMQTLAQTDESVQPRVWLVSQGARAVRGERQIAVTQSPLWGLGRTIAQEMPKL
                     FGGLIDLEAGTTPAQAARLLRDELSALTEERQVAFRQDQRYVCRLVRRPDRYRAERAL
                     KFRADASYLITGGLGALGLQVARWMVEQGARRLILMGRTPLPIRSIWTEVQPDSPFAR
                     QIDAIRQLEAMGASIHLATVDVADEAQLVSFLNAFEREGWPPIRGVIHAAGTLNDRTL
                     FQHDADTFSSTWGPKVIGSWLLHHWLPKDSLDFFILFSSVASLLGSPGQANYAAANSF
                     MDALSCQRKTQGLPALSINWGAWSEVGLAAQAERGGRLAERGFRSISPVEGIEAFSQL
                     VKQDSAQVGVIPIDWKLVRQFNPEIAALPLFSHVAFTEEEQAAMADGRPSPVRTGLNR
                     ERLLAADSSERAQLLEEYLRETSARILGLRQAALDTRRPLNTMGLDSLMAIEFKNAIQ
                     TNLGIMLPMVMIVSGPSIAELVVQMLELLPGQTSDGLEADGSDPFNRPDDLPAPPHSL
                     STEEAEQLLAGLDELSSEQVDRLLAEMLNESR"
     gene            complement(3338..4294)
                     /locus_tag="VGB76_04535"
     CDS             complement(3338..4294)
                     /locus_tag="VGB76_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098202.1"
                     /translation="MLDFSLKLINSPQALISLVARVVAAPAVALDIETVNWWDRQVER
                     VALIQLAYREGGRMRVGVVDALAGLDPEHLRPCLELSPVTKAIHNAAYDAVRLSRHFL
                     IHTSPIHDTMLAARRSGEKRYSLRAQAETHLGLHLDKSEQRGDWSRRPLTIKQLRYAA
                     LDAACTLLLYENQIARGLSANYQLPSMSTSAQTSLPLSDVERPAGGFVEMPKVAALKA
                     VAADGLNTPARALLGVVTELNGRYSPERLAVSAGAERVGLAGWIIDGVLGREADLDED
                     TVRLGVAELIERGLISLNTAQRLEATEAGVQLWNRVKPSADD"
     gene            complement(4516..>5443)
                     /locus_tag="VGB76_04540"
     CDS             complement(4516..>5443)
                     /locus_tag="VGB76_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0098203.1"
                     /translation="KGAVSLWASATTGESTRRQDLLRDKQRIVISFFQYVDKLPGEVE
                     PTDVQKWLLELEEKGVKPGTTYQHACLLSSFYSWALKDPGVGQHVRRNPAKMARPKAP
                     KPYQTESVKALSDEEVMALVGVVRRHALTGDIVGKRDYALLLLYLATGLRRQEAISLH
                     GKNVSVGETLVLEYRAKGGDYRSREVREPQVKEALIDYLSASGRLHALKTDAPLWTRH
                     DRAGKPGEALSSHCFVKNLKKYAQEAGVESFHLHRTRHTFARIVSEFTGDITATQNAL
                     DHQNRSTTRVYVQRIAVKRDLYSNEISKRWSD"
BASE COUNT         1053 a         1707 c         1588 g         1095 t
ORIGIN      
        1 cgccgcgacg aagatgagcg cgccgtcctg ctggattcgc tcggcagcct gtatgccacg
       61 gggcatgagg tgaactggaa aggactgttt actgacggcg ggaaatgcgt ccacgctccg
      121 acgtacactt ggcagcgcga acgtttctgg attgagcctg ccgaacccgc gacggataac
      181 ggaggggtct ggcgagcttc ccgcgacggc cagcgagaac gaccgttgct cggcgcgcac
      241 gtctcacccg cgcacccctc gctcgaacac ctctgggaat tcaggctctc acagaccgaa
      301 ctcccttttc tcaccgacca tcgagtgcag ggcatgatgg tgttgcccgc taccgcttac
      361 atcgaaatgg cgttgggggc agccaggcag gtcttcggcg ggcaggcttt ccgtctcacc
      421 gggttgaagt ttgataaggc tctattcgtg ccggaggcgg cggcgctcat cgtccaactg
      481 ggtttttctc ccccggtcga aggcgtcgtc ggattccaca tctacagccg ccccgaagtc
      541 agcgacggcg gcagacatct ctggacactc cacaccacgg ggcagattca tttcaaccgg
      601 gaggagcagg agcaggagca ggctaacctg cccgtcgagc ctctggagac gattcaggcg
      661 cgctgctata acgaactctc aggcgacgaa ttttatcgcc tcctgaacga tgtcggtcac
      721 gactacggcg ggagttttaa gtctatccaa accctctggc ggcgcgacgc ggaagccgtg
      781 gcgcagtttc acatctctga tgatctggcc gccgaagtgt cctcttacca gtttcacccg
      841 gccatcctcg actgttgcct gcaacttctc ggcgcgacag ttgcgcagaa cgacgagttg
      901 gccgcacggg gcgcgcacgt cccggtggcg attgacgaga ttcgcgtcaa gggtcgcccc
      961 gcagggcagt tgtggagcca cgccgctctg agagttgacg aagaaagccc ggaaaaggga
     1021 agcaataatc tcaaaggcga cgtgtcgctg cgcgacgaag ccggacagat cgtgatcgaa
     1081 ctcgtcgggg ttgaacttca atgcctcgaa gaacattcgg tgcgtgttgc cgccggacgc
     1141 gtcgcggaat ggatgtatga gcctgtgtgg gaagaacagc cgcgcgtgcc ccagagcctt
     1201 gagagccgga cggcgcgcag ggcggacggc gcatggctga tcttctccga ccggcagggc
     1261 atcggcgagg cgttggcgaa cctgttggaa gaggacgggg agaaagtgtt gatggtcgcg
     1321 ccgggcgagg agttcatgcg catcgacgag cgaaactttc tcgccccgtt aacgcaggcc
     1381 gaagacgtgc gggaaattta caaggcggcc tccggtgcgg acggcttcgg ctgtcgcggc
     1441 atcgtccatc tctggagtct ggactcgcgc gggttctcgg aactcacgca cgagacgctc
     1501 aggaaggaat cgctcgacgt gtgcatcagc gtggtgaacg tgatgcagac cctcgcgcag
     1561 accgacgaga gcgtgcaacc tcgtgtctgg ctcgtgtcgc agggcgcgcg ggcggtcagg
     1621 ggcgagcggc agatcgccgt cacacagtcg ccgctctggg gcttgggtcg caccattgct
     1681 caggagatgc cgaaactctt cggcgggctg atcgacctcg aagcggggac caccccggct
     1741 caggccgcgc gcttgctgcg tgatgaactt tccgctctga ccgaagaacg gcaggtcgct
     1801 tttcgccagg accagcgtta cgtctgtcgc ctcgtgcgtc gtccggatcg ctacagagcg
     1861 gagcgcgcac tcaagtttcg cgcggacgcc agctacctga tcacgggcgg gcttggcgcg
     1921 ttgggtctgc aagtcgcgcg ctggatggtc gaacagggcg cgcggcggct catcctcatg
     1981 gggcgcacgc cgctgccgat ccgctccatt tggacggagg tgcagcccga ctctccgttc
     2041 gcgcggcaga ttgacgccat ccgccaactc gaagcgatgg gcgcaagcat ccacctcgcc
     2101 acggtggacg tggcggacga ggcgcagtta gtctcgtttc tcaacgcctt cgagcgcgag
     2161 ggctggccgc cgattcgcgg cgtgattcat gccgccggga cgctcaacga ccgtacgctg
     2221 ttccagcacg acgcggacac tttcagttcc acctgggggc cgaaagttat cggcagttgg
     2281 ttgctgcatc actggctgcc gaaggattct ctcgacttct tcatcctctt ctcttccgtc
     2341 gcgtctctgc tcggctcgcc cggtcaggcc aactatgccg ccgccaattc cttcatggat
     2401 gcgctctcgt gccagcgcaa gacgcagggg cttcccgctt tgagtatcaa ctggggcgcg
     2461 tggtctgaag tgggactcgc cgcgcaggcc gagcgcggcg gtcgtctggc cgagcggggg
     2521 ttccgcagca tctcgcccgt cgagggcatc gaagcgtttt cccaactcgt caaacaagac
     2581 tccgcgcagg tcggcgtcat cccgattgac tggaaactgg tgcggcagtt caatcccgaa
     2641 atcgccgcgc tcccgctctt ctcacacgta gccttcaccg aggaggaaca ggccgcgatg
     2701 gcggatggcc gcccgtcgcc cgtgcggacg ggattgaacc gcgaacgctt gctcgccgcc
     2761 gattcttctg agcgggcgca actcctcgaa gagtacttgc gcgaaacctc cgcacgcatc
     2821 cttggactcc gtcaagcggc tctcgatacg cgcagaccgc tcaacaccat gggactcgat
     2881 tcactgatgg cgattgagtt caaaaacgcg atccagacca atctcggcat catgctcccg
     2941 atggtcatga tcgtcagcgg cccgagcatc gcggaactcg tcgtgcagat gcttgaattg
     3001 ctccccggtc aaacttcgga cggcctggag gcggacggct ccgacccgtt caaccgtccg
     3061 gacgacttgc ccgccccgcc tcattctctc agcacggaag aggccgaaca attactcgca
     3121 ggactggacg agctttcttc agaacaggta gaccgtctgc tcgccgagat gttaaacgaa
     3181 agtagatagg ccggattaca gactcgtccg tttgaaactt tctgtgaccg ctctatgacc
     3241 tgtgtgcctg cgctggtgaa cgtgtgtcaa ccattcgcca gcgcaggcac acagcgcgca
     3301 gtgcggtcac ggagagaaga tcatgccccg gcaattatta atcgtcggcg ctcggcttta
     3361 cacggttcca caactgcaca cccgcctcgg tcgcttccaa ccgctgcgcc gtgttgagac
     3421 tgatcagccc acgttcgatg agttcagcca cacctagcct caccgtgtct tcatctaaat
     3481 ccgcctcccg tcccaacacc ccatcaatga tccaaccggc taaccctacc cgctcggcac
     3541 ccgctgaaac cgccagacgc tccggactgt aacgcccgtt taattcagtg accacgccga
     3601 gcaaagcccg cgcgggtgtg ttcagtccat ctgccgcaac agctttcaag gcggcgactt
     3661 tgggcatctc gacgaagccg cccgcaggcc gttccacatc gctcaggggc agagacgttt
     3721 gggctgatgt ggacatcgat gggagttgat aattggcgga cagtcctctc gctatctggt
     3781 tctcgtacag cagcagtgtg caggccgcgt ccagcgcggc gtagcgtaat tgcttgatgg
     3841 tgagcggacg ccgcgaccaa tcgccacgct gttcgctctt atcgaggtgt aatccgaggt
     3901 gagtctcggc ctgcgctcgc agtgaatagc gtttctcgcc gctccgtcgc gccgccagca
     3961 tcgtgtcgtg tatgggggaa gtatgaatca gaaagtgacg cgagaggcgc accgcgtcgt
     4021 aggccgcgtt gtggattgct tttgtcaccg gactcaactc aaggcacggg cgtagatgtt
     4081 ccggatcgag tccggcaagc gcatcaacga caccgacgcg cattcgtccg ccttcgcggt
     4141 acgctaactg tatcagagcc acccgttcca cctgtcgatc ccaccagtta accgtctcaa
     4201 tgtcgagcgc cacggcggga gccgccacca cccgcgcgac cagagaaatc agagcctgcg
     4261 ggctattaat aagtttgagg ctaaagtcga gcatacgaca ttataattcg caccataact
     4321 taaacacttg cagatcatta ctaattccgt ctcaccagca gcaatgcgaa agataaggct
     4381 agggtctgat aaccaaaaaa cttcgtatag ggctttgcct caaaagtccc aagtaacccc
     4441 aagattctgc gtcagatgtc tttatacaag tgcaaaagga tattcattcg agcaagaagg
     4501 tagttcgcgc ccaagtcagt cgctccacct tttcgagatt tcattactgt agagatccct
     4561 tttgacggcg atgcgctgga cgtagactcg cgttgttgat ctattctggt ggtcgagcgc
     4621 attctgtgtg gcggtgatgt ctccggtaaa ctccgagacg atccgcgcga aggtgtgcct
     4681 cgttcgatgc aagtggaaac tttcgacccc cgcctcctgc gcgtacttct tcaaattctt
     4741 gacaaagcag tgagacgaga gagcctcgcc cggcttccct gcccggtcat gcctggtcca
     4801 caatggcgca tccgtcttca gcgcgtgcag tctccccgat gccgagaggt aatcaatcag
     4861 cgcctccttc acctgcggct ctcttacctc cctgctcctg tagtcgccgc ccttcgcccg
     4921 gtactccaac acgagcgtct cgcccacgga cacgttttta ccgtgcagac taatagcctc
     4981 ctgccggcgt agccccgtcg ccaaatataa gagcaacagc gcatagtctc tcttcccgac
     5041 gatatctccc gtgagcgcat gcctgcgtac cactccgacc aacgccatta cctcctcgtc
     5101 ggagagcgcc ttgaccgact ccgtctggta aggcttcggc gctttcgggc gcgccatctt
     5161 cgccggattc ctgcgcacat gctgacccac tcccgggtct ttcaaagccc acgagtagaa
     5221 ggatgagagc aggcaggcat gctggtacgt cgtccccggt ttaacacctt tctcttccaa
     5281 ttccagcaac cacttctgaa cgtccgtcgg ctccacctct cccggcagtt tgtcgacgta
     5341 ctggaagaat gagatgacga tacgctgctt gtctctgagc aagtcctggc gtcgcgtgct
     5401 ctcacccgtc gtggctgatg cccaaaggga gacagcacct ttt
//
LOCUS       DASWNS010000138         1928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_161002, whole genome shotgun sequence.
ACCESSION   DASWNS010000138 DASWNS010000000
VERSION     DASWNS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161002"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VGB76_04545"
     CDS             complement(<1..444)
                     /locus_tag="VGB76_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098204.1"
                     /translation="MTRDTVKKNRTRMRLTLPGLAAAIICAAALLGEGSTGSASAQRM
                     RGRRVPAPQSVSVDRGTVARAESYTGDRFNYFTETPRGARVVAVNRPRAEALRAIDAG
                     LADLFDAARRNNYRARLNYSDYVVFIARADRTRDSTGAYSPDMAFG"
     gene            complement(656..865)
                     /locus_tag="VGB76_04550"
     CDS             complement(656..865)
                     /locus_tag="VGB76_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098205.1"
                     /translation="MKHLQKILAFAVIFSALATGTLAAQPQRDQREPPPKDPKVFDKK
                     DKEPKREEPRREQRDKPKENRRDKP"
     gene            complement(1198..>1928)
                     /locus_tag="VGB76_04555"
     CDS             complement(1198..>1928)
                     /locus_tag="VGB76_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098206.1"
                     /translation="VAYLPRNVAPEILSITTLPTGVALMPAIQIQVDPNVEASGFDPS
                     LIGPVPQVPPRRAFQRGAVALQWQAEDRNGDTLEYTVYYRAQTESAFHMLKEKLRDNF
                     YTVDGAALGDGRYIFKVVATDAPENSIGAALSGERLSEPVEVDNTAPLVRTTGEPVLA
                     AGTDRVRVSFVVEDARGRVRRADVSVDGGAWRAVFPEDGIADSPRETFALDLPLAGAG
                     EHTISLRGFDASGNVGSARALVRR"
BASE COUNT          335 a          596 c          590 g          407 t
ORIGIN      
        1 gccgaaagcc atgtcgggcg aataggctcc ggtgctgtcg cgcgtgcggt cggcgcgtgc
       61 gataaagact acgtagtcgc tgtagttcag gcgtgcgcgg tagttgttgc gacgcgctgc
      121 gtcgaagagg tctgccagcc ccgcgtcaat ggcgcgcaag gcttcggcgc gcgggcgatt
      181 cacggcgacg acacgtgcgc cgcgcggcgt ctccgtaaaa taattgaaac gatctcccgt
      241 gtaagactcg gcgcgcgcaa cggtgcctcg atccacgctc acgctctgcg gcgcgggcac
      301 ccgacgccct ctcatccgct gcgcgctcgc gctgcccgta gaaccttcgc cgagcagtgc
      361 ggcggcgcaa atgatcgcgg cggccaagcc cggcagggtc agcctcatgc gtgtgcggtt
      421 cttcttcaca gtgtctctcg tcatctgctt catcgtctga tagcccccgt gtgaatttat
      481 aaaagttgat gcccgcccaa gaatgccgga cgctttcgtc gccgacgttt ttgagggggc
      541 atcaatcgtg ttgaaaatgg gtcgaactat ttaccgggcg acgcgctact ccttttagtt
      601 ctccgccgcg cgtcccacgt caaagctgcg cgaaaagagc gaaggcggtt gaactttagg
      661 gcttgtccct tcggttctcc ttcggcttgt cgcgttgctc gcggcgcggc tcctcccttt
      721 taggctcctt gtcctttttg tcgaagacct tcgggtcttt cgggggaggc tcgcgctgat
      781 cacgctgcgg ctgcgcggcc agcgtgccgg tcgcaagcgc agagaagatc actgcaaacg
      841 ctaaaatttt ctgcaaatgt ttcataacta aaactcttcg cctcctcatg tccttgattg
      901 tttcgaggat gcgcatggga gggagaagag agacgcgcat tcgtctcgct tcgatactgt
      961 gcaacatgga tgccacaaac gactcgacgg catcctattg cttttacagt ccatagttta
     1021 ccgcgttacc cgcaaaggcg cacgctttcc gtcgcaactc acacggtatc gtgtaggaat
     1081 ggggaaaggt gcaggggcga ggggtaaagg aaagcttgtc ggcgaagtta tcgcaggctc
     1141 tgtttttacc ttccccctta cctctccatc tttccctcat ctccgaatcc tctccggcta
     1201 tcttcgcacc agcgcgcgtg cgctgccgac gttgccgctg gcgtcgaaac cgcgcaggga
     1261 gatcgtgtgc tcgcctgcgc ccgcgagggg caggtcgagc gcaaaggttt cgcgcgggct
     1321 gtctgcgatg ccgtcttcgg gaaagaccgc gcgccacgcc ccgccgtcaa cgctcacgtc
     1381 cgcacgacgc acgcgcccgc gcgcatcctc caccacaaaa ctcacgcgca cgcggtctgt
     1441 gcccgctgcg agcacgggtt cgcccgtggt gcgaacgagc ggggcggtgt tgtcaacttc
     1501 cacgggttcg ctcaggcgtt cgccggagag cgcggcgccg atggagttct ccggcgcgtc
     1561 cgtcgccacg actttgaaga tgtaacgacc gtcgccgagc gcagcgccgt caaccgtata
     1621 gaagttgtcg cgcagttttt ccttcaacat gtgaaacgcg ctttcggtct gcgcgcgata
     1681 atagactgtg tattcgagcg tgtcgccgtt gcggtcttcg gcctgccatt gcagcgcgac
     1741 cgcgcctctc tggaaggcgc gacgcggcgg cacctgcggc acgggaccga tgagcgacgg
     1801 atcaaagccg gacgcttcga cgtttggatc aacttgaatt tgaatcgcgg gcatcaaggc
     1861 gacgcccgtg ggcagcgtcg tgatcgaaag gatttccggc gcgacgttgc gcggcaagta
     1921 agcgacgc
//
LOCUS       DASWNS010000139         8930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_394832, whole genome shotgun sequence.
ACCESSION   DASWNS010000139 DASWNS010000000
VERSION     DASWNS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8930
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394832"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1567)
                     /locus_tag="VGB76_04560"
     CDS             complement(83..1567)
                     /locus_tag="VGB76_04560"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY0098207.1"
                     /translation="MEDEESRAIDNPVTLMSGESVPFGFTVSITRATANEEFAYSFRI
                     KGTDPSDGSTKTLDYHLADVEHNSYLNLFQKLAQDFGLRLPRNRRLAAEPTSRAKYRA
                     LLTENLAPGILYGYGDPAVLRVEEEAGGEVGVWYYLVVTSNDAPDSFPIIRSRNLINW
                     EFTGFVFPEGKKPDWAADGKYVSDFWAPEMHKVRGEFRVYFVARDRKTRELSIGLAKS
                     ARPTGQFIAGKEPILSGNVIDPHVFVADAETAFLYWKEDTNDIWPSRLTHLLHEHPHL
                     ITELFPEREDQITASLIQTLWPWIRSLQPMERFFAQQVLVEAVTSAFTAFHDRLSKLS
                     DRQSTGNALEQVRAVLQVLRTPVYAQRLSSDGLHLIGERVKIIENDLAWEAHLIEGVW
                     LAEQQGRYYLFYAGNDFSTASYGIGVAIADSPLGPYRKMAEPLLRSTNEWAGPGHPSV
                     AVAPDGELLLFFHAFFPERTGYKEFRALLAARIEFAAERVLLDR"
     gene            complement(1814..2521)
                     /locus_tag="VGB76_04565"
     CDS             complement(1814..2521)
                     /locus_tag="VGB76_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0098208.1"
                     /translation="MSEPNSPRPHNSLLAALPDVEYQRLVPHLKPVSLSQGEVLHEAD
                     TPAQFVYFLEEGVASLSVSSEEGKELMLSIAGDESVIGERAIFKEGHFIVRCEMLTDG
                     RGHRMEPTTFEEEFNRGERLHQLVLNRIEARMTETAQTALCNQMHSVEQRLARWVLTL
                     ADRLHTDELHITHDLIASMIGVRRAGITDAMGTLREAGLIESGRGTVTILDREKMEAQ
                     ACECYAIIKEAIETFAS"
     gene            complement(2847..3578)
                     /locus_tag="VGB76_04570"
     CDS             complement(2847..3578)
                     /locus_tag="VGB76_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0098209.1"
                     /translation="MSTFETPSPPTSNRILNALPLAEYKRLSAYLEPVEMGVGEVLYH
                     PHEPISHAYFPNRGTVSVVCQFEDGKSVEAAMVGNEGMFGVCVFLGSITSPMEAVVQL
                     PGDALRMRFDVLQAEFKRGEHLQDLLLRYTQAFIIQIAQTAACNRAHPIDGRLARWLL
                     MSQDRAHSPELQLTHEFIAQMLGTRRAGVSEAAGKLQEEGIIKYRRGHVSVLLREELE
                     GRSCECYPIVEKEFQRLLGVSGQAR"
     gene            3821..4219
                     /locus_tag="VGB76_04575"
     CDS             3821..4219
                     /locus_tag="VGB76_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098210.1"
                     /translation="MNNRDERRGTILLVEEDKDVRPNLRRNLQKDGYRVSLAIDEEDA
                     LERVGGGYVPVDLVLIDLVDKSADEVLQVGRRIREHGKYDGHTPLVVLAEEYGADVEG
                     TDVNVCGNDWISYLENRDQLNNLLARLLGR"
     gene            4468..4893
                     /locus_tag="VGB76_04580"
     CDS             4468..4893
                     /locus_tag="VGB76_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098211.1"
                     /translation="MFEETISQREARPTIFLVEEDDDARPGLTANLRGQGYRVLVAAG
                     LQDAHDWVSGESPVNADLVLINLVRKTPEESVRLGRELREHAKYDGHTPLVVMPEKVP
                     RELEGTDVNVSGNDWICYYEEVTPQLRKLLARLLDKSSS"
     gene            complement(4926..5084)
                     /locus_tag="VGB76_04585"
     CDS             complement(4926..5084)
                     /locus_tag="VGB76_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098212.1"
                     /translation="MLLKEETGLNVLPFGLLELSANGTILYYRPDEKEDQQANNTDLV
                     GHNILTGV"
     gene            complement(5074..5481)
                     /locus_tag="VGB76_04590"
     CDS             complement(5074..5481)
                     /locus_tag="VGB76_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098213.1"
                     /translation="MTYKPEQNYRPVVLVVEDHDDTRFMLRMLLEHDGYAMLEASDGL
                     EGIEIAIRQHPDLVLMDGSLPGLDGLSATRRMREQESLRDVPIVALSGHDGAEFQAAA
                     RAAGCDAYITKPLDFAEFRSTLGRLLHSYSHAA"
     gene            5952..6254
                     /locus_tag="VGB76_04595"
     CDS             5952..6254
                     /locus_tag="VGB76_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEY0098214.1"
                     /translation="MMPEQFRSDYVSERVRADDEREEQKLNSATGARLTPAEAEEVWP
                     QVLEHKWYLSERLGRDVGLRVAALDYFKNVRRIAKRDELREVTPPALPFRRPFGYR"
     gene            6346..7038
                     /locus_tag="VGB76_04600"
     CDS             6346..7038
                     /locus_tag="VGB76_04600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0098215.1"
                     /translation="MIKAVIFDVDGTLVDSNDLHVEAWQVALRGCGKEVAYEELHEQM
                     GKGGDQLMPVFCTPEELDRFGEELERSRTELFTTDYLPRVCPFPKVRELFERIKADGL
                     AIALASSAKEEELAQHKRNLRIEDLLETATSADDAGRSKPHPDIFQAALEGIKGVEPS
                     EAVVVGDTPYDAIAAAKAGMRTIGLLSGGFTEEALRAAGAVEIYEGVADLLARYDESL
                     LGAKAATVVASR"
     gene            complement(7059..7592)
                     /locus_tag="VGB76_04605"
     CDS             complement(7059..7592)
                     /locus_tag="VGB76_04605"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY0098216.1"
                     /translation="MDESAGAAPIATADPMAYNVEAIAKLEHRELHGRSMGEKVSDFF
                     VSIMGSMSFLVFHVVWFAVWFMVNLRAIPGVAPFDPFPFGILTLIVSSEGVFLAIFIL
                     ISQNRMTRQSDKRAHLDLQVSILAEQEMTMMLRMQRQLCEHLGVEVDTVREEAKELLE
                     ETDVHRLVSTLEDKLPG"
     gene            complement(7763..>8930)
                     /locus_tag="VGB76_04610"
     CDS             complement(7763..>8930)
                     /locus_tag="VGB76_04610"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0098217.1"
                     /translation="PLRRILHRAPNVEVILGEVTGFDTAARIVKLNVGAQLSYDFLIV
                     AAGARHSYFGHDEWEKDAPGLKTIEDALEIRRRILLAFEVAEREAYLTGSHEPLNFAV
                     IGAGPTGVEVAGAIAGIARRALAKDFKAIDTTQAHVMLLEGAPRVLGMYPEELSRKAE
                     EQLIELGVDVRKGSLVTAVEAGRIKVGEEWISTSVTVWASGVAASPLGKELGVPTDRA
                     GRVAVETDLSLPGHPEIFVIGDMAAIKNPDGKPVPGLGAAATQEGKAAAANILCDLRG
                     EKRRPFAYSDRGSMATIGRNRAVAEFGRWHLSGLTAWLLWLLVHVFLLIGFRNRLWVM
                     REWIWAYFTRERSARLITGTTNAEPQTPQQATTTTSPVTLPVALAAKKVQGAGQ"
BASE COUNT         1856 a         2516 c         2650 g         1908 t
ORIGIN      
        1 aaaggtagat tgaacttata caaatcttcc ggtgtctcat gtgaggcaat cgtatccgag
       61 tagatgcttt tgatttccag gttcatcggt caagcagaac gcgttcagcc gcaaattcaa
      121 ttcgcgcggc gagcagagca cggaactctt tatacccggt tctttcagga aagaaggcat
      181 ggaagaagag gagcagttcg ccgtcaggcg ctacggcaac agatggatgt cccggtccag
      241 cccattcatt agttgagcgt agcagcggtt ccgccatttt tctgtagggt ccgagcggag
      301 aatccgcgat ggcgacgcct attccgtaac tggccgtaga aaagtcgttc ccggcataaa
      361 agaggtagta tctgccttgc tgctcggcga gccacacgcc ctcaatcaag tgagcttccc
      421 acgctagatc gttctcaata atctttaccc gctctccgat gagatgcagg ccatcggatg
      481 ataacctctg cgcatacacg ggagtcctca aaacctgtaa cacggcgcga acctgctcaa
      541 gagcatttcc agtgctttgc ctgtccgaca acttactgag gcggtcgtgg aatgctgtaa
      601 aggcggaagt aacagcttca accagcacct gttgagcaaa gaaacgctcc ataggttgaa
      661 ggctccgaat ccaaggccat agagtctgaa tgagagaggc tgtaatttga tcctctctct
      721 cgggaaaaag ttcggtaatg aggtgaggat gttcgtgcag taagtgtgtc agacggctcg
      781 gccagatgtc gttggtatct tccttccagt ataagaaggc tgtctcggcg tctgccacga
      841 agacgtgcgg atcgatgacg ttgccgctca gaatcggctc cttcccggca ataaactgcc
      901 cggtcgggcg agcggacttt gccagaccga tggagagttc gcgggttttt ctgtcgcgcg
      961 ctacgaaata gactctaaac tcgcccctca ccttatgcat ctcgggcgcc caaaagtcgc
     1021 tgacgtattt accatccgcc gcccaatccg gcttcttacc ctcggggaac acgaagccgg
     1081 taaattccca atttatgagg ttgcgtgatc tgatgatagg aaacgagtcg ggggcatcat
     1141 tcgaggtgac gacgaggtag taccatacgc ccacttcacc gcccgcttct tcctcgacgc
     1201 ggagcacagc cggatcacca tagccgtata gtatgccggg agccagattc tcagtcagaa
     1261 gggcgcgata ctttgctcta gaggtaggct ctgccgccag acgccgattg cgcggcagcc
     1321 gaagcccgaa atcctgtgca agtttctgga acaggttcag gtaggagtta tgctccacat
     1381 ctgcaaggtg gtaatcgagg gtctttgtcg aaccgtcgga cgggtctgtt cccttgatcc
     1441 taaacgagta tgcgaactct tcattcgctg ttgcccgcgt gatgctgact gtaaagccga
     1501 aggggacact ctcccccgac atcagcgtga caggattatc tatcgcgcga ctttcttcat
     1561 cttccatctg cgtccctcgt tgaatgagtt gtcgttgaag ctgataggat agctcttaat
     1621 atggccgtgc gcatttattc agaggagacg ctcgcgctgg caggataggc gggggcactg
     1681 tatttcgaac ggtagggttg caaaagatat tttagttacg acgaccggtg gcgagcaggg
     1741 caggtaattt caagatcagg tgtggggaga aagttgttgc gccactaacg ggagacacta
     1801 ccatgatctg ttttcaagag gcgaaagtct caatcgcttc tttgatgatg gcgtaacact
     1861 cgcaggcttg cgcttccatc ttctcgcggt cgagaatcgt gaccgtcccg cgccctgact
     1921 cgatgagtcc ggcctcgcgc aaagtcccca tcgcatccgt gatccccgcg cggcgtacgc
     1981 ctatcatgct ggcgatgaga tcatgcgtga tgtgcaattc atcggtgtgc aggcggtcgg
     2041 ccagcgtcag cacccaccgc gccagccttt gctctactga gtgcatctgg ttgcagagcg
     2101 cggtctgcgc ggtttccgtc atgcgcgcct cgatccggtt gagcaccaac tggtgcaaac
     2161 gctcgccccg gttgaactct tcttcaaacg tcgtgggttc catccggtgg ccgcgcccgt
     2221 ccgtgagcat ctcgcaacgg acaatgaagt gtccctcttt gaagatagcc cgctcgccga
     2281 tgacgctctc atccccggcg atgctcagca tcaactcttt accctcctcg ctgctcacag
     2341 acagcgaggc cactccctct tcgagaaaat agacgaactg cgccggagtg tcggcttcgt
     2401 gcaggacttc gccctgactg agcgacacag gtttcaagtg tgggacgagg cgttgatatt
     2461 caacgtcggg caaggcggct aaaagcgagt tgtgcgggcg cgggctgttt ggttcagaca
     2521 tgcagttatc cttcatttat gcgagagata aggaataacc cctggtgcga tgataacggc
     2581 gggagcatag catatccgga gcggagctat tctcaatgtt tagggtgtcc gcccgacccg
     2641 cgccatacag agcgggcaag ggctgtagtt cggtgtcggc gtggacagcg tgacgacggc
     2701 ggacggacgc cgctcacgca catcgcgccg gctgcccttc gtggacaggt tgcagagtgt
     2761 gcgcgttcgg gcggcgcgct ccagcagatg gtattggtaa tcaccgggga aggtggcgaa
     2821 gctagccatc agtatctctc cagcggctac cgggcttggc cgctcacgcc gagcaggcgt
     2881 tgaaattcct tctccacgat ggggtagcat tcgcacgatc tcccctcaag ctcttcccgc
     2941 agaaggacac tgacgtggcc gcgcctgtat ttgatgatgc cttcctcctg aagtttaccc
     3001 gccgcttcgc tgactccggc gcggcgcgtg cctaacatct gcgcgatgaa ctcgtgcgtc
     3061 agttgcaact ccggcgagtg cgccctgtcc tgagacatca ggagccagcg cgccagtcgc
     3121 ccgtcaatgg ggtgggcgcg gttgcaggcc gccgtctgtg cgatctggat gatgaaggct
     3181 tgcgtgtaac ggagcagtaa atcctgtaag tgctcgcccc gcttgaactc cgcctgcaac
     3241 acgtcgaaac gcatgcgcag ggcgtcgccc ggcagttgca cgacggcttc catcggcgac
     3301 gtgatgctcc cgagaaagac gcagacaccg aacatgcctt cattgcccac catcgccgcc
     3361 tccacgctct tgccgtcctc gaactggcag acgacggaaa ccgtgccgcg attcggaaaa
     3421 taggcgtgcg agatgggttc gtgcgggtgg tacaggactt cgccgacgcc catttcgacc
     3481 ggctctaaat aggcggagag gcgtttgtat tctgcgagcg gcagggcgtt tagaatccgg
     3541 ttcgacgtgg gcggggatgg agtttcgaaa gtggacatca gatagccttt ctgctggcgg
     3601 ttaaacttca acgtagagcg cgcacggcag caggcggagg gcgtctggct gaacctagtg
     3661 tcgatgactt ttattagcga ataggcaggt acaccatagc ccatgcgccc accgcgcaca
     3721 gtacggttgc atacgcaact ctatttatgt ctgcgcgcgc tgccgtaccg aaaagtgggc
     3781 gtcggagtgt catagtgcaa tgtttctgcg agggggcatt atgaacaatc gagacgagcg
     3841 ccggggcacg atcctgctgg tggaagagga caaagatgtg cgccctaact taaggcgcaa
     3901 tctacagaaa gatggttacc gggtatcgct ggcgatagat gaggaagacg cgctggaaag
     3961 agtcggcggc ggctatgttc cggtcgatct cgtgctgatc gatctggttg ataagtcggc
     4021 ggatgaggtc ttgcaggtcg ggcgcaggat acgcgaacac gggaagtatg acggccacac
     4081 tccgctcgtc gtgctggctg aagagtacgg cgcggacgtg gaaggtacgg atgtcaatgt
     4141 ctgtggaaat gattggatca gttacctcga aaaccgcgac cagttaaata acctgctcgc
     4201 ccgtctgctc ggtagataat cgaatcaggg cgaccatata gttgattcac ctgagttgcg
     4261 ccccgatttg aatatagccg ggaagttggc ggcaagagca cagcgcgcgg tgtgctcaac
     4321 gttttaactg ttcgctgctt atcatttgta agggcatgaa gctgttcttt tggagttaac
     4381 cacttgatgt caggaagagc ttaataaatt gctctttgat gtcgtcagct ctgttttgaa
     4441 cgcgaccgga gacagggaga atcctgaatg ttcgaagaaa ctatctcgca gagagaagcc
     4501 cgcccgacca tctttctcgt tgaagaagat gacgatgccc gtcccggttt aactgcgaac
     4561 ctgcgcgggc agggctaccg agtgctcgtg gcggctggcc tccaagacgc gcacgactgg
     4621 gtgagcggcg aaagtccggt taacgccgat ctagtcttaa tcaatctggt gcgtaagacg
     4681 cccgaagagt ctgtgaggct cgggcgcgag ttacgcgagc atgccaagta tgacggccac
     4741 actccgctcg tcgtgatgcc ggaaaaggtt ccccgagaat tagagggcac ggacgttaat
     4801 gtgagcggga acgattggat ctgttactac gaagaagtca cacctcagtt gcgaaagctg
     4861 ctcgcacgcc tgctcgacaa atcatcgagc taggacgcta cgcggttccg ccttcgtatg
     4921 acccgtcaga ccccggtgag gatattgtgc ccgacgaggt ctgtattgtt agcctgttga
     4981 tcttcttttt cgtccgggcg gtaatagagg atggtgccgt tggcggagag ttcgagcagg
     5041 ccgaagggaa gaacgtttag tcctgtctct tctttaagca gcatgtgaat aagagtgtag
     5101 caggcgaccg agcgtactcc tgaactcggc gaagtcgagc ggcttcgtga tgtaggcgtc
     5161 gcacccggcg gcgcgcgccg cggcctgaaa ctccgccccg tcgtgcccgg agagggcgac
     5221 gatgggcacg tcgcgcagcg attcctgctc acgcatccgg cgggtcgcgg agaggccgtc
     5281 gagtcccggc aggctgccgt ccatcagtac cagatcgggg tgctggcgga tggctatctc
     5341 aatcccttcc aacccgtccg aggcttccaa catggcgtaa ccgtcatgct ccagaagcat
     5401 tctgagcatg aagcgcgtgt cgtcgtgatc ttcgacaacc agcactaccg ggcgatagtt
     5461 ctgttcaggt ttgtatgtca tgagagtgcg acatataccg tctgcttcga gcaagccgct
     5521 gtgccgtagt tggtgatagt tagtgtcggg ttagaaagtg aaaaggcgag cctcgcgatg
     5581 aagcccgtcc cttcttcccc ttgtaccgtc gcgccactgt gatgagtcag tccgggagca
     5641 cgtgtgttca gtagattcgc gacactgatc tccgagcctc tccggcactc ataccgattg
     5701 acgtgaacgg gcagaatata gcgggtgcaa cgccgctcgt ctgtacgctt catggcacac
     5761 tttgttgagg tgggaatgaa aaaagaaggg tgtcctacgt cgagtcaccg gacgtggaga
     5821 tgttggaagc gatagtggca gagccgccgg tatcgtgacc atatctctat gtgttcgtaa
     5881 cctctgcgag aactgatatt catcatactt ctttcattat ttgacgctgg tgcgtcgatc
     5941 aagggggttt gatgatgcct gaacaattca gatcagatta tgtctccgag cgcgtacgtg
     6001 cggatgacga aagagaagaa cagaaactca attctgccac aggggcgagg ctcacccctg
     6061 cggaggcgga agaggtctgg ccgcaggtgc tggaacacaa gtggtacctg agcgaacggc
     6121 tggggcgtga tgtcggcctg agggtcgccg ctctcgacta tttcaagaac gtccgcagga
     6181 tagcgaagcg cgacgagttg cgcgaggtga cgccgcccgc tctgcccttc cgacgcccgt
     6241 tcggctacag ataaaaaaac tccgtgaacc gaagcgcagg ttaaagcccc tttgggagtg
     6301 cgggtggcgg ttgcccgttc cgttgacgga cttaaggaga agattgtgat taaagctgtg
     6361 atattcgatg ttgacggcac gctcgtcgat tccaacgacc tgcacgtcga ggcgtggcag
     6421 gtcgccctta gaggctgcgg caaagaggtg gcttatgaag agttgcacga acagatgggc
     6481 aagggcggcg accagttgat gcccgtcttc tgcacgccgg aagagttgga caggttcggc
     6541 gaggagttgg aacggagccg cacggagttg ttcacgacgg actacctgcc gcgcgtgtgt
     6601 cccttcccaa aggtgcgcga actcttcgag cgcatcaagg ccgacggtct ggcaatcgcc
     6661 ctcgcctctt cggccaaaga ggaggagttg gcgcagcata agcggaacct acgcattgaa
     6721 gatttgttgg agacggccac ctctgccgat gatgccggga ggtcgaagcc gcacccggac
     6781 atctttcagg ctgcgctgga aggcatcaaa ggcgtcgagc cgtcggaagc cgtcgtcgtc
     6841 ggcgacacgc cctacgacgc gattgccgcc gcgaaggcgg gcatgcgcac cattggtctg
     6901 ctcagcggtg gttttacgga agaggctttg cgtgcagccg gggctgtcga aatatacgaa
     6961 ggcgtggccg atctgctcgc gcgctacgac gagtctctgc tcggggcaaa agcggcgacg
     7021 gtcgtagcca gccggtaaaa gctgccgtcg catgattctc atcccggcaa tttgtcttcc
     7081 agcgtactca ctaacctgtg aacatcagtc tcctccagca gttctttcgc ctcttctctg
     7141 acggtatcca cctccactcc gagatgctca cagagttgtc gctgcatgcg gagcatcatc
     7201 gtcatctcct gctcagcaag gatgctgacc tgcaagtcca gatgcgcccg cttgtcggac
     7261 tgccgcgtca tgcggttctg gctgattaag atgaagatgg cgaggaagac gccttccgac
     7321 gaaacaatca gcgtcaagat gccgaaggga aatggatcga acggggcgac gccgggaatc
     7381 gcacgaaggt taaccataaa ccagaccgcg aaccacacga cgtgaaatac gagaaaggac
     7441 atgctgccca tgatggagac gaaaaagtcg ctgacctttt ctcccatcga gcggccatgc
     7501 aattcccgat gttcgagttt agcgatggcc tccacgttat aggccatcgg gtcggctgtg
     7561 gcgataggag cggctccggc agattcatcc atccttgttc ttccccttgt tctttcacat
     7621 cacatgcgat taacgcgtct gacagggagc ggcagatgtt ccgcatgatc gagggctgcg
     7681 gcttgccgac aaactcccac gatgatcgca ccgggttatc aaccccgcct ctcacattat
     7741 cttttccgcg cgcggcaaat cttcactgcc ccgctccctg caccttcttc gccgctaacg
     7801 caaccgggag cgtcacggga gaggttgttg ttgttgcctg ttgcggcgtt tgcggctcgg
     7861 cgttcgtagt gcccgtgatg aggcgcgcgc tgcgctcacg cgtgaagtaa gcccatatcc
     7921 attcgcgcat cacccagagg cggttgcgga atccgatgag caggaagacg tgaaccagca
     7981 gccagaggag ccacgcggtc aagcccgaca ggtgccagcg cccgaactcc gccacggcgc
     8041 ggttgcgccc gatggtcgcc atgcttccac ggtcgctata cgcgaatggc cgccgcttct
     8101 cgccgcgcag gtcgcacagg atattcgccg ccgccgcctt gccttcctgc gtcgccgccg
     8161 cacctaaacc ggggacgggc ttgccgtcag gattcttgat cgccgccatg tcgccgatga
     8221 cgaagatttc gggatgaccg ggcagactca gatcagtttc gaccgccacg cgacctgccc
     8281 tgtccgtcgg cacgcccaac tctttaccga gcggtgaggc ggcgactccc gaagcccaca
     8341 cagttacgct cgtcgaaatc cattcttccc cgactttgat ccgccccgcc tcgaccgccg
     8401 ttaccagact ccccttgcgt acatcgaccc ccaactctat caactgctct tcggctttgc
     8461 gcgatagttc ttccgggtac atgccgagca cgcgcggcgc gccttccagc agcatgacgt
     8521 gcgcctgcgt cgtgtcaatc gccttgaagt ctttcgccag cgcgcggcgc gcgatccctg
     8581 caatcgcccc ggcgacctct acgcccgtgg ggcctgcacc gatcacggcg aagttcagag
     8641 gctcatggct gcccgtcagg taagcctcgc gctcggcgac ttcgaaggcc agcaggatgc
     8701 gacgccgaat ctccaacgca tcttcaatcg tcttgagtcc gggcgcgtcc ttttcccatt
     8761 cgtcgtgccc gaagtaagag tggcgcgcac cggctgcgac gatcaggaaa tcgtatgaga
     8821 gttgcgctcc gacattcagc ttcactatgc gcgccgccgt gtcgaagccc gtcacctcgc
     8881 ccaggatgac ttcgacgttc ggcgcgcgat gcaaaatgcg gcgcagcggg
//
LOCUS       DASWNS010000140        26821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_316725, whole genome shotgun sequence.
ACCESSION   DASWNS010000140 DASWNS010000000
VERSION     DASWNS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 26821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26821
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316725"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VGB76_04615"
     CDS             complement(<1..1059)
                     /locus_tag="VGB76_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0098218.1"
                     /translation="MFLLEVIPEKQPALLSLIYVAGLGLMLLLLGLSLVLGMRRRRSA
                     LAAVAPADLPTEVRQRLGVTSTNRGLYALRWLFLILALTVFGFHVYWARYAEERNDRF
                     SDLNYKDLRNRRLSESTLRGWILDRSGSLERALALYKRGDNGRIVREYPLDREMAQLF
                     GSERGDAGIERALFGVQSGAAPEAIDVLFERDIKQPGNLDVRLTIDSELQKAAVEQLR
                     DRHGAVVILNPQTGEVLAMYSNPTYTLAEVQDESNWIKLDADKRNSPLVNRSLRSYYI
                     PGSTFKTVTMIAQYLAGTQNEKYLCSGNGYYAQPGAKVIYDDQGPAEVHGHIGVDKAY
                     EVSCNQYFAQAAVKMGAER"
     gene            complement(1159..1986)
                     /locus_tag="VGB76_04620"
     CDS             complement(1159..1986)
                     /locus_tag="VGB76_04620"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0098219.1"
                     /translation="MDKTEKKESVPKRDEALAERLLRRVLDRVGAVVDRSLGRTVEPQ
                     SGFTTTRLIERMNRLIDERSRNDAKRGRIAPHVMRLKIEWGTHSEAPPESVRELENEV
                     LAAAIDHINDQRLRTLAPVEVETIVDIFTTGIAVDPGYGAFEEELQREDEERERAAAG
                     VAVPSSPGATKNVSVYARIQIGKESEEKSLSFKPGGKRLSVGRATDCDLSLAHPSVSK
                     IHAAILMNREGTLLVSDTGSVNGTYINGRRINYGEARQLEDGDVVGFGDVEVRFRKQ"
     gene            complement(2309..3103)
                     /locus_tag="VGB76_04625"
     CDS             complement(2309..3103)
                     /locus_tag="VGB76_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY0098220.1"
                     /translation="MITSRQNPLIQHARAVSRGTSKELLFIEGLRLCEEAARSELACE
                     EVIHTEKFAADPRAARLLAALERLKPRFHLVSEGVFASLSDTKSPQGIVLLAQRRATT
                     RATFEKSLHPNPLIVVLHGVNNPSNAGAMLRVAEAAGATGVIATAGTTDLLSPRALRG
                     AMGSGFRLPLWTNADFAEVVSWCGAHGIRTVSAYLAADKGHTALVWTEAYAVIMGAEA
                     GGLEDEEARAADERIRIPMQPPVESLNVAVALAVILYEAARQRATA"
     gene            3266..3628
                     /locus_tag="VGB76_04630"
     CDS             3266..3628
                     /locus_tag="VGB76_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098221.1"
                     /translation="MKRRVIVLVEDLFFAAKIRTTAEHLGIEAVFPRSPEALREASEG
                     NAPALFIVDLHLQRDDPFLIARGLKADAELSATPLVGFFSHVQVELQRRAEASGFDRV
                     LPRSAFTKRLPEILQGRI"
     gene            complement(3815..4834)
                     /gene="aroF"
                     /locus_tag="VGB76_04635"
     CDS             complement(3815..4834)
                     /gene="aroF"
                     /locus_tag="VGB76_04635"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY0098222.1"
                     /translation="MLIVMKADATERDIAEVVRVVTELGLRAHPLPGANRTAIGVTGN
                     HGAVDATRFETLSGVAEAIRVTKPYKLISLDLKPEKTVVRVGDAAIGGDELALIAGPC
                     AIESRAQAFAIAETVRRSGARFFRGGAYKPRTSPYAFQGLGEEGLKILADVREAYGLK
                     IVTEALDEHGVDLVERYGDMIQLGARNMQNFSLLKRAGRSPLPVLLKRGLAATLDEWL
                     LAAEYIMAEGNYNVVLCERGIRTFAQHTRNTLDLAAIPAVRRLSHLPVVIDPSHGTGK
                     NYMVTPLARAGVATGADGLIIEVHDQPERALSDGAQALTLAQYEQLVTEVRAIHEVIA
                     PAPVG"
     gene            complement(5127..7190)
                     /gene="argS"
                     /locus_tag="VGB76_04640"
     CDS             complement(5127..7190)
                     /gene="argS"
                     /locus_tag="VGB76_04640"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY0098223.1"
                     /translation="MNLLELQKRLSARVQRTIEEKFVSFPAVQGEIPPRIELGDVAFP
                     VAFEVAKRLKQEKGERANPRAIAEALKAALEAEAGVARVEVAGAGYLNVFYDRAQSLQ
                     HLVAASEAAVAHTQTEAGDNSKVIVEHTNINPNKAAHIGHLRNGVLGDTFVRVLRATG
                     AAVEVQNYIDNTGVQVADVVVGFMHIEHMTLADVQRLDESLPPERPFDYYCWDLYASV
                     GLFYRRGDAEAKEAPELLKLRGETLHAIEAGDNPTAEIADYVATRIVQCHLKTMARLG
                     IRYDVLPRESEILHLHFWDHAFTRLKETGTIRFETEGRNAGCWVMPSDAHTGTDEHDA
                     DKIIVRSNGTVTYAGKDIAYQLWKLGELGLDFYYKPFQQYDDGHTVWVTTASPPTAEA
                     QVAYQPPKFGGGSIVYNVIDSRQSYTQDIVQRGVQAVVPGLARDPSVHLSYEMVALSP
                     AASEELGFELTPEDRARPHIEMSGRKGLGVKVDDLIDRLEANALAEVESRHAELTAAE
                     KVETAHTIATGALRYFLLKWTRNSLIAFDFKEALSFEGETGPYCQYAAVRANSIFRKL
                     DEASYARAREFARTLSETEAGAAEVRQVLTGETGDELWSLVTLAARLDETIAAAATQA
                     EPAYLAKYTFQLAKAFNLFYHRHRIIAEENETRRHVLVNIADLARRQLTAALSTLGIH
                     VPERM"
     gene            7689..8411
                     /locus_tag="VGB76_04645"
     CDS             7689..8411
                     /locus_tag="VGB76_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098224.1"
                     /translation="MPGSSKNNKRPEQSSQLTETHTALKLDLVSETGEKILEIIEASY
                     QISVKGIDSSSIRVRNLSNRNITALGIIWTVKFTDAGQCLLKQIADYRLHKDMVKAKG
                     IRPFAPYEEKFIPRLTNESLDEEQVIKSVGVEISFVEFEDSSGIGIEKSEMYEQLLSK
                     RKGAEIYKQWIESDYRDNAQNVARVKEKLISDGLPRDKELENSWVEQGALIYRQWMRD
                     ILNNKGEHALQEQIHQQFKRQK"
     gene            8930..9646
                     /locus_tag="VGB76_04650"
     CDS             8930..9646
                     /locus_tag="VGB76_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098225.1"
                     /translation="MPLRHVPEQRHVLLRRELRRLRSHIFPDNTASLMPLPDNEPCGG
                     GCIVDPDTNTCASPILIDVAGDGFHLTDAARGVLFDINGDFTPEHLGWTTHGTDDAWL
                     VLDRNNNGTIDDGRELFGNYTPQPPPPAGAQPNGFLALAVYDWTTNGGNADGIIDAQD
                     PIFSRLRLWQDTNHNGVSEATELHSLSSLHVVSLHLNYKESKRVDEHGNEFRYRAKLN
                     DAEGTKVTRWAWDVFLVKAP"
     gene            9865..10623
                     /locus_tag="VGB76_04655"
     CDS             9865..10623
                     /locus_tag="VGB76_04655"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEY0098226.1"
                     /translation="MPTQFDPALLTELRERLRAARRVFVLTGAGVSAESGVPTFRGGG
                     NSAVWKGVPFDVISSAGMLARDLPAVWEWFDYRRKLLRDLQPNGAHRTLAVWQEKFAR
                     LSLATQNIDGLHAAAGSRDVLELHGNIWTARCTGCGARKSLLEEGSGDSEPERPPICR
                     ECGEAMRPDVVLFGELLPAGVFEQAATEAAGCDLCFVIGTSSLVYPAMRLPEIALEGG
                     AYTVEINPEATPFSALSDVAIQGRAGEVLPRLEF"
     gene            10624..11853
                     /gene="larC"
                     /locus_tag="VGB76_04660"
     CDS             10624..11853
                     /gene="larC"
                     /locus_tag="VGB76_04660"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEY0098227.1"
                     /translation="MRTLYFDCFAGASGDMILGALVAAGADARALTEQLALLDVADFE
                     VAFEKVDRSGISATHARVQTVEEQHHRHLSAILKIIDGSRLSEAVKARAARIFERLGE
                     AEARVHNVPVERIHFHEVGAMDAIIDVVGACIGFELLGIERFVASPLHVGSGTVEMAH
                     GRFPVPPPAVLELLSDAPIYATDIKGELVTPTGAAIISTVSAEFGALPRMRVAATGYG
                     AGTREYKDFPNVLRVIIGETEAVETAVEKLSRREELIMIETNLDDTTPQVLGYVSERA
                     FALGALDCFLTPVQMKKNRPGVLVSILCRPAAQEALTQLLFAETTTLGVRSYEVERRA
                     LAREFVSVETEFGRISVKVARGGDGRVSNVAPEFEDCRAAALGAGVPLHLVEAAARAA
                     FDARRADEKGKDESERG"
     gene            11834..12415
                     /gene="folE"
                     /locus_tag="VGB76_04665"
     CDS             11834..12415
                     /gene="folE"
                     /locus_tag="VGB76_04665"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY0098228.1"
                     /translation="MKAKEVDLKKIGAGVRLILEGIGEDPERAGLQRTPQRVAEMYAE
                     LTAGMREDPREHVTPLPGDSHDELVIVKDIHVASICEHHLAPFVGRCHIAYIPKGGRI
                     LGLSKLARLADTFAHRLQVQERLTSEIADTIFDGLKPLGVMVVIEAEHTCMTLRGVKK
                     PGALTITSAIRGAFRKDPRTRAEAMSLIQGHRS"
     gene            12492..14675
                     /gene="metG"
                     /locus_tag="VGB76_04670"
     CDS             12492..14675
                     /gene="metG"
                     /locus_tag="VGB76_04670"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY0098229.1"
                     /translation="MKTFYITTPIYYANSLPHLGHLYTTMVADCVARYRRQRGYETYF
                     LTGTDEHGINVQRAAARSGRTPKEHVDYIVGELKESFAAFGLDGEHGGYDIFMRTTEP
                     FHYEAVAEFWRRVARAKTPAGREAIYKGHYEGWFCAPCATFKTEDEYTKPEREGDAPL
                     CLIHEAPLDRVAEESYFFRLSDYAEALLALYESRPDLIRPEARRNEVVSFVKRGLQDL
                     SVSRLKTSVSWAIPVPDDPAHTIYIWLDALSNYITALGWGSGGGGRENSFDKFWPAVH
                     LIGKDILRQHAIYWPAFLMAAGIEQPRLVYAHGIWLDHQGRKMSKTLGNSIELPTLRR
                     HFATDIVRYFCLREMAFGQDSKFGYEAVIERSNSDLASGLGNLSSRTLTMIGRYCDGL
                     IPAPDIDEEKRLQAKRAGVDADGGELASALEHARDSFVQHFEDYAFARALETIWTVIA
                     RADKMISDAKPWELAKDETQRQTLHAVLYRSAETLRWLAVLLHSTMPEATRAIWRQLG
                     QTDDIAKNDPAELQWGGLQAGTRIGEVAPVFPRIDKAKIMSEIEKENEAKQAASAETT
                     AATQAGTHATEADAVPGSANAAETKAEPPQRGATEGDAAPATQAAAPEGVATLIGIED
                     FVKVEMRVGEILTAERIPKSDKLLRFTIDLGEAAPRQVLAGIAEYYEPEKLVGRKIIV
                     VSNLKPRKLRGFESQGMVLAASIGDEGRPVLATFNEDVPNGARLK"
     gene            14843..15637
                     /locus_tag="VGB76_04675"
     CDS             14843..15637
                     /locus_tag="VGB76_04675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY0098230.1"
                     /translation="MFIDSHAHIDGAEFDADREEVLARAREAGVVSILNVGTGDPHGG
                     ELERAVAVAERYEGVYAAVGVHPHDARLFDDAAAERVYELARSRERVIAWGEIGLDYH
                     YDNSPREVQREVFARQLRLARELKLPCIIHSREADEDTVATLRAEWSGAAQGGIMHCF
                     GGGYEMAASVLELGFMISFAGNVTFKKAEALREVARRVPLERMLIETDCPFLTPVPFR
                     GRRNEPARVVETARHLAELRGLEVAEFGRITTSNFQRFFNLPGAAQ"
     gene            15840..16337
                     /locus_tag="VGB76_04680"
     CDS             15840..16337
                     /locus_tag="VGB76_04680"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY0098231.1"
                     /translation="MAQQNSFDIVSQVDRAEITNALDQTMKEVRQRFDFKGSAATVVL
                     EKEELILTAEDETKLRNMNDILQQKLVRRSVPLKAFNYGKIEAALGGTVRQHVAIQQG
                     IPTDKAKEIVKFIKDTKAKVQATIQGDVVRVTGKDRDTLQDIIAKLKTKDFNINMQYT
                     NYRSN"
     gene            16417..17436
                     /locus_tag="VGB76_04685"
     CDS             16417..17436
                     /locus_tag="VGB76_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY0098232.1"
                     /translation="MSISSHLAAIEEPQVAATRIFSLIAAELAAVEEEFERQARSNIQ
                     VIAYIGDYLRASGGKRVRPALTILSTYAAGGDAAQPNVIRLATVMEFLHTATLVHDDI
                     IDNAETRRNRPSINSRFGNQTAVLMGDWLYMSAFEASLAERSLPVLDILTAVTRRMTE
                     GELLQLTMLGRSDVTEEQYFDILMRKTAYLFSACCEIGGMLGTSDPETQLALRDYGMN
                     LGVAFQLVDDLLDFTATDDVLGKAAGVDLIEGKLTLPLIYLLESDREQRAAVQAVMRE
                     GNYETTSRASLMHAAEGAGAIERARQRAHDFATAARKSLDVLPHTKYRDALSSIPTFI
                     LERDR"
     gene            17561..18142
                     /locus_tag="VGB76_04690"
     CDS             17561..18142
                     /locus_tag="VGB76_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098233.1"
                     /translation="MPDDNLLATLEQSLRQTRATRNRLAARLADLELEAEKLRTELGE
                     MDAIATQTEAAMFRILSSVLNTVPAKSSPSDAEIEVALRRDEAQVRNTHPPAYQTVPR
                     STLPPVRADVEVTSERFLDRTIPQATAMLLREAAAPLHVNEIYNRLLEGGFQFTGHNP
                     TISIAVSLNRNRRFRKVAPGTFDLTIRDAAQAS"
     gene            18278..18655
                     /locus_tag="VGB76_04695"
     CDS             18278..18655
                     /locus_tag="VGB76_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098234.1"
                     /translation="MRKLKTGYTFDPHAGATYSFEHMPLADAEERAQQTETPHIVEAV
                     NWTKSEGGEVHYNRYYAHNFDEALGLAVRLRIGFEEGVEEVLSIRPATKSEAADGGVA
                     SAHFLELMPLAISSIDKKEGTKK"
     gene            18652..18876
                     /locus_tag="VGB76_04700"
     CDS             18652..18876
                     /locus_tag="VGB76_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098235.1"
                     /translation="MSEENRLIALSTLFEDVSSVRGKIPIPVIKMLKAKAGDMLAFER
                     RPDGTVVVRKSTAAERKAGGGGGRKGSGKK"
     gene            18873..19127
                     /locus_tag="VGB76_04705"
     CDS             18873..19127
                     /locus_tag="VGB76_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098236.1"
                     /translation="MSGTGDEEKLNPLELEQRGLDVPGDEGLVEGEPGAGTDANYSAP
                     PPRVKSREAGDLAGATSTHDGAPGKLEQTGGDGTAPTRVK"
     gene            19210..20250
                     /locus_tag="VGB76_04710"
     CDS             19210..20250
                     /locus_tag="VGB76_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEY0098237.1"
                     /translation="MRAMILAAGYGTRLWPLTLDRAKPALPFMGRPLVGYVAEYLARF
                     GCDEIIVNLHHRPESVRAALGDGSDFGVRLSYIEEPEILGTSGAIDNARAYLEGDTFI
                     VVNGKIATDIDLGAALETHRRERALATLVLRPNVERERYSIVEVEGHAVRGFGGYPHA
                     PPARTNAAELQLGVASRGEVEPGAGTAPLMFTGIQILEPRIFDYIPRGVFSHSVTDVY
                     LPAIARGERIAAHVSEGMWYELSTIQRYRDISLTLMRLEGRDIISTGAGTIVEAGAEV
                     HEAILWENVRIEAGARVSRAIIGAGVRIGRGEVVVDAAVVRAALVEGEERPAKALAGE
                     VRGDNFVVPLAQ"
     gene            20399..21451
                     /locus_tag="VGB76_04715"
     CDS             20399..21451
                     /locus_tag="VGB76_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0098238.1"
                     /translation="MSHPSGITANAFVASAHERLSDYLVRANLDAGKILALTPDASTR
                     EYFRIPWKKRGSTAVAAVYPEPFDPEIHPFLDVTRLFVAARLPVPEILDVDAVHGIIV
                     QEDLGDQQLRRVFETVSEDEREDYLERAISLIADIQAATPLAFERDSIASRLAFDEAK
                     LFWELEFFLEHYFRSYRKESLKHAEEAELRAELNDLAAELAARPRVLCHRDFHTSNLI
                     VDRENRLRIIDHQDARMGPVSYDLVSLLLDRQTGVPSLAEIRERRLYFLEERRQRGLD
                     ALDPDEFGREFRLMTVQRCLKAVGTFSYQSGVCGREEVYAHFINPVLSIVLQALEWLA
                     RFPLLRETIRARLGGK"
     gene            21530..22894
                     /gene="asnS"
                     /locus_tag="VGB76_04720"
     CDS             21530..22894
                     /gene="asnS"
                     /locus_tag="VGB76_04720"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEY0098239.1"
                     /translation="MATENIEQEEQAAEAKSLPQVYINQLSEHAGAEVTLRGWLYNLR
                     SSGKLLFLQLRDGTGIVQCIVFKKGVAAELWDALKSLGQESALKIHGTVRADERAPGG
                     YEIDVSGAEVLQAAEGYPITPKEHGTEFLMDHRHVWLRSRRQHAILKVRHTIVRAVRD
                     FLDNDNFTLADAPVLTPAACEGTTTLFEVDYFNDEKAYLTQSGQLYNEATAAAFGRSY
                     CFGPTFRAERSKTRRHLTEFWMVEPEMAFATLDDVMNLAERFLANIATRVLEERQEEL
                     KVLERDTAKLELIVPPFPRLHYDDAVKLLHEGYERGELEAKFEWGGDFGAPDETYISK
                     QFEKPVMIHRYPAAIKAFYMARDPEREELALGVDVLASEGYGEVIGGGERATSLEFLE
                     AQIKAHDLPREAFEWYLDLRRYGSVPHGGFGMGIERCTAWMCGIEHVRETIPFPRMLY
                     RLRP"
     gene            23008..23559
                     /locus_tag="VGB76_04725"
     CDS             23008..23559
                     /locus_tag="VGB76_04725"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEY0098240.1"
                     /translation="MSSTQERIVTIFGGSKCGEDCAEYAEARRAGQLLAEAGFTICTG
                     GYLGVMEAASRGAHERGGRVLGIVMNQFKKEPNRFLTDKVATPHFYDRLQHLIVRSVG
                     FIALRGGMGTVTELSLVWNKIQTRVIEPRPLVLLGDCWPPVVEAWRRHLIVSEEDVRM
                     LDLAQTPEEAVRIIIEKSKGVSV"
     gene            23625..24491
                     /locus_tag="VGB76_04730"
     CDS             23625..24491
                     /locus_tag="VGB76_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEY0098241.1"
                     /translation="MSSAQAAPNVWGGGAGSSGDVGGGAASGGVGGGGVGHGIHIDAD
                     ARGQGRGYSFEILHQPSFALAVVQLQAEQSILAEAGAMVSMSANVELQSQMKGGLMGA
                     LKRAVGGESAFVSTFTARGGPGEVSFAPGAPGDIAAIEMSNQMFMVQSSSYLAGDASL
                     TVDTKWGGAKTFFGGEGLFVMQVSGTGLLLLTSFGAIHRRTLRPGERYVVDTGHLVAW
                     EASMQYALRKAASGFFRTMVSGEGIVAEFTGPGELLIQTRNLAAFAGLLKPFFPSQGS
                     SGGSGFNINLGG"
     gene            24517..25482
                     /gene="rocF"
                     /locus_tag="VGB76_04735"
     CDS             24517..25482
                     /gene="rocF"
                     /locus_tag="VGB76_04735"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEY0098242.1"
                     /translation="MSALSDQTKSSLINIQPGEGRRVSIIGVPLSFGAGTAGMDLGPA
                     AMRVARLNQRLALLGYEVRDLGDMRLEHPKMLAAPHDRAKYLREVGIVCEELAAEVQR
                     VMRAGEFPIVLGGDHSIAAGSIAGAASIFHERQQPLGLIWFDAHADMNTPETSLSGHV
                     HGMPLAALLGYGAPEMTHIGGFSPKLDPRYCAHIGARDLDPAERDLIRQLGIRFFTMR
                     EIDERGMSACVDEAIAIAKRAAGGYYVTLDVDALDPGDAPGSGTVVRGGLTYRESHLA
                     LEKIAEAGGMIAFEVVEINTALDINNRTAELGVELILSAMGKTIL"
     gene            25646..26785
                     /locus_tag="VGB76_04740"
     CDS             25646..26785
                     /locus_tag="VGB76_04740"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEY0098243.1"
                     /translation="MKRKIRVGVIFGGRSGEHEVSLRSGRAVIDAIDHRKYEVIPIAI
                     TKTGAWLAPAAAMELLPRQTAELLSRGMSENREALTIVGDPSHSGLVAVGVEGGRPAP
                     PALPVDVVFPVLHGTYGEDGTIQGLLEMADVPYVGCGVLASSCGMDKVTMKMLFREAG
                     LPICKHLWFLRAEWEREHAGILRRVARELGFPCFVKPANLGSSVGISKATNRRTLEAA
                     IDLAARYDRKIIVEEGLDAREIECAVIGNDDPEASLPGEYIVHDQQGAFLDYTEKYSN
                     TGHVEFVVPAPIPKSLATRIRRMSVAAFKSIDGTGLARVDFFLRRDTNELLINELNTN
                     PGLTEVSGYPKLWAASGLPFSQVIERLIQLALERHEDKSRNETSL"
BASE COUNT         5435 a         7968 c         8231 g         5187 t
ORIGIN      
        1 acgctccgcg cccatcttca ccgccgcctg cgcgaaatac tggttgcagg aaacttcgta
       61 agccttatcc acgccgatgt ggccgtgaac ttccgccgga ccctgatcgt cgtagatgac
      121 ctttgcgccg ggctgcgcgt agtagccgtt accgctgcac aggtacttct cgttctgcgt
      181 cccggccaga tattgcgcga tcatcgtaac ggtcttaaac gtcgagccgg gaatgtagta
      241 gctgcggagc gagcggttga cgagcgggct gttgcgtttg tccgcgtcga gcttgatcca
      301 gttgctctcg tcctgtactt cggcgagcgt gtaggtcggg ttgctgtaca tggcgagcac
      361 ttcgcccgtc tgcggattca ggatgacgac cgcgccgtgc cggtcgcgca actgctcaac
      421 ggcagccttc tgcaactcgc tgtcaatcgt caaacgcacg tcgagattgc cgggctgttt
      481 gatgtcgcgc tcgaagagca cgtcgatggc ttcgggagcc gcgccgcttt gcacgccgaa
      541 gagcgcgcgt tcgatgcctg cgtcgccgcg ttccgagccg aacaactgcg ccatctcgcg
      601 gtcgagcgga tactcgcgca cgatgcgccc gttgtcgccg cgtttgtaga gcgcgagcgc
      661 gcgttcgagg ctgcccgaac ggtcgagtat ccagccgcgc agggtggatt ccgagaggcg
      721 tcggttgcgc aggtctttgt agttgagatc ggagaaacgg tcgttgcgtt cctctgcata
      781 gcgcgcccag tagacgtgaa agccgaagac cgtgagcgcg aggatgagaa agagccagcg
      841 cagcgcgtag aggccgcgat tcgtggaggt gacgccgagc ctctgccgca cctcggtcgg
      901 caagtctgcg ggcgcaaccg ccgccagagc cgaacgtctt cgcctcatgc cgagcacgag
      961 cgacaggccg agcagcaaga gcatcagccc cagccccgcc acgtagatca aactcaacag
     1021 cgcgggctgc ttctccggaa taacttctaa aagaaacata tttttaagtg acaagcgaca
     1081 agtgacaagc gacaggatgt aaagacaagt aaaagatagt tttttccttt taactcgtca
     1141 cccgtcactt gccactcgtc actgctttct gaatctgacc tcgacatcgc cgaatccgac
     1201 gacatcgccg tcttcgagtt ggcgcgcttc gccgtaattg atgcgcctgc cgtttatata
     1261 tgttccgttt accgaacctg tgtcggaaac gagcagagtt ccttcgcgat tcatcaaaat
     1321 cgcggcgtga atcttcgaca cgctcgggtg cgcgagcgac aggtcgcagt ccgtcgcgcg
     1381 cccgacgctc aggcgtttgc cgcccggttt gaaggagagc gacttttctt ccgactcctt
     1441 gccgatctgg atgcgcgcgt acacggagac atttttcgtc gcgccgggag acgagggcac
     1501 ggccacgccc gccgccgcac gctcgcgctc ctcatcctcg cgctgcaact cctcttcaaa
     1561 cgcgccgtag cccggatcaa ccgcgatgcc cgtggtgaaa atatcgacga tagtttccac
     1621 ctcgacgggt gcgagcgtgc gcaggcgttg atcgttaatg tggtcgatgg cggcggcgag
     1681 cacctcgttc tccaactccc tgaccgactc cggcggggct tccgaatgcg tgccccactc
     1741 gatcttgagt cgcatgacgt gcggtgcgat gcgcccgcgc ttcgcatcgt tgcgcgagcg
     1801 ttcgtcgatc aggcggttca tgcgttcgat gaggcgggtg gtggtgaaac cgctctgcgg
     1861 ttcgaccgtc cgcccgagcg agcgatcaac caccgcgccc acgcgatcca gcacgcgccg
     1921 caacagtcgc tcggctaacg cttcgtcgcg cttcggcacg ctctcttttt tctctgtctt
     1981 atccatgcaa gtctatacgg ccacatgccg acgaggttcg aagatcatca agctgctcag
     2041 agtaaactac gcgcgttaaa acggcgcggc gcggggggaa tgaattgtag atattttcaa
     2101 gcgcgcacta ttttaagggg aaaagggtaa aagtaaaaag gggaaagata gaaaccgccc
     2161 tctggagaca gtctcgaagg gaaaagcctg aaaggcgaaa ccgggaaact atgcgaaaac
     2221 aggccgaaat tgtttcgcgc cctttcggga cgcagccgga ttcgcgtggt tcgctttttt
     2281 gcgattcgtt cggatttcgc cgccgtcctc acgcggtcgc acgctgccgc gcggcttcgt
     2341 agagtatcac cgcgagcgcg accgcgacgt tgaggctttc gacgggcggt tgcatcggga
     2401 tgcgtatgcg ctcgtcagcc gcacgcgctt cctcgtcctc caaccctccg gcctccgcgc
     2461 ccatgatgac ggcgtaagcc tccgtccaga cgagagcggt gtgacctttg tccgccgcga
     2521 gatacgcgct caccgtgcgt atgccgtgcg cgccgcacca tgagacgact tcggcaaaat
     2581 ccgcatttgt ccacaggggg aggcgaaagc ccgaccccat cgcgccgcgc aatgctctgg
     2641 gagagagcag gtctgtggtg cctgcggtgg cgatcacgcc cgtcgcccca gcggcctcgg
     2701 ccacgcgcag catcgctccc gcgttcgacg gattattgac gccatgcagc acgactatca
     2761 gcgggttcgg atgaagcgac ttttcaaacg tcgcacgggt ggtcgcgcgt cgctgcgcca
     2821 gcaggacgat cccttgcgga ctcttcgtgt cgctcaggga ggcaaagacg ccttcactga
     2881 cgaggtggaa gcgcggcttg agacgttcga gcgcggcgag caggcgcgcg gcgcgcgggt
     2941 ctgcagcaaa cttctccgtg tggatcacct cttcgcaggc gagttctgaa cgggcggcct
     3001 cctcgcacag gcgcaagcct tcgatgaaga gcaactcctt cgacgtgcct ctgctcacgg
     3061 cgcgcgcgtg ctggatgagt ggattttgac ggctcgtgat catcgaggct ttcgacggtt
     3121 tgtgcggcca gaccgtagtt ttatcattaa ctcaaaagtc ggtcggagtt tatgatgaaa
     3181 agcgtccgtc gtaaacttgt tgccggagag caggcgcggc gtcgggtttt tcacatttgt
     3241 ttagttgtcg agagagcgtg tgaggatgaa gagacgtgtg atcgtgttgg ttgaagacct
     3301 ttttttcgcg gcgaagattc gcacgacggc ggagcatctc ggcatcgaag ccgtctttcc
     3361 gagaagcccg gaggctctcc gcgaggcgtc cgaaggaaac gcgcccgcgc tctttatcgt
     3421 tgacctgcac ttgcagcgcg acgacccgtt tctcatcgcc cgcgggctga aagctgacgc
     3481 ggaactctcg gcgacgccgc tcgtcggctt cttctcgcac gtgcaggtag aactgcaaag
     3541 gcgcgcggaa gcatcgggct tcgaccgcgt actgccgcgc tcggccttca ccaaacgact
     3601 gccggaaatt ttgcaggggc gcatttaaac gctcgcctgc ttaaaccgcg caccaactca
     3661 tcaagcgtgc atcgcatgag cttaaggacg ctgctatccg gcagtgtcaa attctcaaaa
     3721 ctctcaacgc cgaactcgca cggcaactct accgtgcgag ttactgaacg acgacgcctt
     3781 tgccgccagc cttcgttcct gcgcgagcct tgatctaccc gacgggcgcg ggcgcgatca
     3841 cttcatgtat ggcgcgcact tccgtcacga gttgttcata ttgtgcgagc gtgagcgcct
     3901 gcgctccgtc agagagcgcg cgttccggtt gatcgtggac ttcgatgatc aacccgtcag
     3961 cccccgtggc gactccggcg cgtgccagcg gcgtcaccat gtagtttttg cccgtgccgt
     4021 gcgaaggatc gatcacgacg ggcaggtgcg agaggcggcg cacggcgggg atggccgcga
     4081 ggtcgagcgt gttgcgcgtg tgctgcgcga aggtgcggat gccgcgttcg cacagaacga
     4141 cgttgtagtt gccttcggcc atgatgtact cggcggcgag cagccattcg tcgagcgtgg
     4201 cggcgaggcc gcgtttgagc agcacgggca ggggcgagcg tccggcgcgt ttgagcaggg
     4261 agaagttctg catgttgcgc gcgccgagtt ggatcatgtc gccgtagcgt tccacgaggt
     4321 caacgccgtg ctcgtcgagc gcctccgtca cgatcttcaa gccgtaggct tcgcgcacgt
     4381 ccgcgagaat ctttaaaccc tcttcgccaa gcccctgaaa agcgtagggc gaggtgcgcg
     4441 gcttgtacgc gccgccgcga aagaaacgcg cgccgctgcg ccgcaccgtc tcggctatcg
     4501 caaacgcctg cgcgcggctc tcgatggcgc aaggcccggc gatgagcgcg agttcgtcgc
     4561 cgccgatggc cgcatcgccg acgcgcacca ccgtcttttc cggtttcagg tcgagcgaga
     4621 tcaatttata aggcttggtg acgcgaatag cttcggccac gcccgacaag gtttcaaagc
     4681 gcgtggcatc caccgcgccg tgattgccgg tgacgccgat ggccgtgcga ttcgctccgg
     4741 gcagcgggtg cgccctcagt ccaagttccg tcacgacgcg gacgacttct gcgatatcgc
     4801 gctccgtcgc atcggctttc atcacgatca gcatatgaat ctccagaaac agtaacgagt
     4861 gacaagtgac gagttaaaag ctaaagacaa gttttaagag cctcgatcaa aacaacgatt
     4921 cagccactca aacctttgcg cctcgcgtct taactctgcg cctttgcgtg aattccgctc
     4981 tcgtaaaagg cgcaaagtga aaacgggaag cgcaaagaaa gagagccggt aattttgcca
     5041 cgcacgctga gtttaaactt gtcactcgtc acttgtcact catcactctc ttcttaaact
     5101 cgtcactcgt cacttgtcac tgtcttctac attctttcgg ggacatggat gccgagcgtc
     5161 gagagcgcgg cggttaactg gcggcgtgcg aggtctgcga tgttgacgag cacgtggcgg
     5221 cgcgtttcgt tctcttcggc gatgatgcga tggcggtgat agaagagatt gaaagccttt
     5281 gcgagttgga aagtgtattt cgccagatag gctggttcgg cctgcgtcgc cgccgccgcg
     5341 attgtctcat ccagacgcgc cgcgagcgtg acgagcgacc agagttcgtc tcccgtctcg
     5401 cccgttaaaa cctgccggac ttccgccgcg cccgcttccg tctcgctcag cgtgcgcgca
     5461 aactcacgcg cgcgcgcata actcgcttcg tccagcttgc ggaagatcga gttggcgcgc
     5521 acggctgcat actggcaata ggggccggtt tcgccctcga aggagagcgc ctctttgaaa
     5581 tcgaaagcga tgagcgaatt gcgcgtccac ttcaagagaa agtagcggag cgcgcccgtg
     5641 gcaatcgtgt gcgccgtctc gaccttttcg gcggcggtga gttcggcgtg gcgcgattcc
     5701 acttcggcga gggcgttggc ttccagacga tctatgagat cgtcaacctt cacgcccaga
     5761 cctttgcgcc cgctcatttc gatgtgcggg cgcgcgcgat cttccggcgt caactcgaag
     5821 ccgagttctt cggaggccgc aggcgacagc gcgaccatct cataagagag gtgcacgctc
     5881 ggatcgcgcg cgaggccggg cacgacggcc tgcacgccgc gctgcacgat gtcctgcgtg
     5941 tacgactggc gcgagtcgat cacgttatag acgatgctcc cgcccccgaa cttcggcggc
     6001 tggtaagcca cctgtgcctc cgctgtcggc ggcgacgccg tggttaccca gaccgtgtgg
     6061 ccgtcgtcgt attgttgaaa cggcttgtag taaaaatcga gtccaagctc tccgagtttc
     6121 cagagttgat aggcgatgtc cttgcccgcg taggtgaccg tgccgtttga gcggacgatg
     6181 atcttgtctg cgtcgtgctc gtcggttccg gtgtgtgcgt cggagggcat cacccagcag
     6241 cccgcgttgc gcccttcggt ttcgaaacga atcgtgcccg tctctttgag acgcgtgaag
     6301 gcgtggtccc agaaatgcag gtggagaatc tccgattcgc gcggcagcac gtcgtagcgg
     6361 atgcctaatc gcgccatcgt cttcaagtga cactggacga tgcgcgtggc aacgtaatcg
     6421 gctatctctg cggtcgggtt gtcgcccgct tcgatggcgt gcagcgtctc gccgcgcagt
     6481 ttcaacaact ccggggcttc tttggcctcc gcgtcgccgc gccggtagaa gaggccgacg
     6541 ctcgcgtaca agtcccagca gtaataatcg aacggcctct cgggcgggag cgactcgtcg
     6601 aggcgttgca cgtccgcgag cgtcatgtgt tcgatgtgca tgaagccgac gacgacatcc
     6661 gccacctgca cgcccgtgtt gtcgatgtag ttttgcactt cgaccgccgc gcccgtcgcg
     6721 cgcagcacgc gcacgaacgt atcgccgagc acgccgttgc gcaggtgtcc gatgtgcgcg
     6781 gccttgttcg ggttgatgtt cgtgtgctcg acgatgactt tggagttgtc gcccgcctcc
     6841 gtttgcgtgt gtgcgaccgc cgcctccgac gcggcgacga gatgttgcaa actctgcgcg
     6901 cgatcataga aaacgttgag gtagcctgcg cccgccactt cgacgcgcgc cacgcccgct
     6961 tcggcttcga gcgcggcctt caacgcttcg gcgatggcgc gcgggttggc gcgctcaccc
     7021 ttctcctgtt tgagccgctt cgccacctcg aaggcgacgg ggaaggccac gtcgccgagt
     7081 tcgatgcgcg gcggtatctc gccctgcacg gcggggaagg aaacgaactt ctcttcaatc
     7141 gtgcgctgca cgcgcgcgct caatcttttc tgtagttcaa gcaggttcaa tgatgcgtgt
     7201 gtccttatct tgggagtcgg agagcgatag cgcggcttca ctgtcggagc atgccgtcgc
     7261 tgcaaaagtg gaaagtataa cttacgcgca gcacagacac taaatgtgat agatgaacgg
     7321 agacgaacag gacagtttaa gtcttactca tggagacaaa attttattga cagtgacaag
     7381 tgataggcgt ataggctgta ggcggcgcac ggcttctatt gacgaaactc tcaccagctt
     7441 ccatcaatcc gcgaaacctt gtttttgttc ccacgccttg agactagccg atatgaaaaa
     7501 gttgctctgg tttctagtcg tacttccgct gatggctgtt ttcacaaaca attgaaacgc
     7561 tcggtgaggc ggctctcaat cgaggtaact ctcatctaca gggaagtcat aagttatgaa
     7621 atacaaaaaa gcgatgccac tcgtacttgt cggctccttt atgttttttt ttgacattta
     7681 tcttacccat gccgggttct tcaaaaaata acaaaagacc tgagcagagc agccaattga
     7741 cggagactca taccgccctg aaactagatt tggtatcgga aaccggcgaa aaaattttag
     7801 aaattatcga ggcttcctac cagatatcag taaagggaat agatagttcg tcaattcggg
     7861 tgaggaatct cagtaatcga aacattaccg cgttaggcat catctggaca gtaaaattca
     7921 cagatgccgg tcaatgtcta ttaaagcaga tcgcagatta cagacttcat aaagacatgg
     7981 tgaaggctaa ggggattaga ccttttgcgc cttacgaaga gaaatttatt ccacgtttga
     8041 caaacgaatc tttggatgaa gaacaagtga ttaagagcgt tggcgtggaa atctcttttg
     8101 tcgagtttga agattcgagc ggcattggca tagaaaagtc tgaaatgtac gaacaacttc
     8161 tctccaagag gaaaggagcc gaaatttata aacaatggat agagagtgat tatagagata
     8221 atgcccaaaa cgtggctcgg gttaaagaaa agctgattag tgatggcctg ccgagggata
     8281 aagagttgga aaatagctgg gttgagcaag gggcattgat ctacagacag tggatgcgcg
     8341 acatccttaa caataagggt gaacatgccc tgcaagaaca aatccaccag caatttaaaa
     8401 ggcaaaaata gatcaacgtt attagtgtgt ttcttgagca gcgaaatggt ggacaggcat
     8461 gaaactgaaa atattgctgg cactaacttg cataataata ttcatcatct ctgtattttc
     8521 tcagacaagg tctaaactgt ataaagcaaa aatgcttggg aacaaatact gtagatgcgt
     8581 atgggaatac aggctgccaa atttcattcg attataaaga ttgcgatggg cgtactcata
     8641 actcccgtcc gcagtgtacc aatggcattc atggacaaag ctcttgccaa tgcacttgca
     8701 ctgcggcacc aaacaacggg tgggcaattt cctactctct tccagatgac tcgaatgtaa
     8761 gcgaatcgaa aagttgtctc ggttgcccga caccaacacc aacgcctaca ccttgtccta
     8821 caccaaatac gcctcaacca tccgggaagt gtagttggga ttctcgatat tgcacttgga
     8881 attgcgatta cgcaggaggc ggctgcacga cgccaggttt tgacggaagt tgcccttacg
     8941 gcacgtaccc gagcaacggc atgtgctgct ccggcgggag ctgcgacggc ttcgctccca
     9001 catcttccct gacaacacgg cctcgctgat gccgctcccc gacaatgaac cgtgcggcgg
     9061 aggctgtatc gttgaccccg acacgaacac ctgcgcctcg cccatcctca tagacgtggc
     9121 gggcgacggc ttccacctca ccgacgccgc gcgcggcgtg ctcttcgaca tcaacggcga
     9181 ctttacgccc gaacacttag gctggacaac tcacggcacg gacgacgcat ggctcgtgct
     9241 cgacagaaac aacaacggca caatagacga cgggcgggaa ctgttcggca actacacgcc
     9301 gcagcccccg ccgcccgcag gcgcacagcc caacggcttt ctcgccctcg ctgtctacga
     9361 ttggacaact aacggcggca atgccgacgg tatcattgac gcccaagacc cgatcttctc
     9421 tcgcctgcgc ctctggcagg acacgaatca caacggcgtg tcggaagcga cggagttaca
     9481 cagtttgtcg tcgttgcatg tggtaagcct gcatctcaac tataaagaat cgaagcgggt
     9541 ggacgagcac ggcaacgagt tccgctaccg tgcgaagttg aacgatgcag agggcacgaa
     9601 ggtcacacgt tgggcgtggg atgtgttcct cgtcaaagct ccttaagtcg aaattaaatc
     9661 gaagcgcgtc gtgaaaccgc acgcaaggtc tgtgcagcct tgcgtgcggt ttcgcctgtg
     9721 ggcagtcatc tccgaacttg actctcacgc gggggcggca tccgctgcca tcgtgaagtt
     9781 cgagcgaggc tgcaacccgc gcacacaaag ctcctaactt gacacttcgt cggtcggctc
     9841 tgctaccatc gccgcctgat ttcgatgccg acacaattcg atccagcttt gctcacagag
     9901 ttgcgcgagc gactgcgagc cgcgcgtcgc gtctttgtcc tgaccggcgc gggcgtgtcg
     9961 gctgagagcg gcgtgcctac gtttcgcggc ggcggaaatt ccgccgtctg gaagggcgtc
    10021 cccttcgacg tgatctcgtc cgcagggatg ctcgcgcgcg acctgcccgc cgtctgggag
    10081 tggttcgact accggcgcaa actgctccgc gacctgcaac cgaacggcgc gcaccgcacg
    10141 ctcgctgtct ggcaagagaa gtttgcgcgc ctgagcctcg ccacgcagaa catagacggg
    10201 ctgcacgcgg cagccggttc gcgcgacgtg ctcgaactcc acggcaacat ctggacggcg
    10261 cgttgtaccg gatgcggcgc acgcaagtct ctactcgaag agggaagcgg cgacagcgaa
    10321 ccggaacgac cgcccatctg ccgcgagtgc ggtgaggcga tgcgccccga cgtggtcttg
    10381 ttcggcgaac tgctgcccgc gggagtcttc gagcaggcgg cgacggaagc ggcgggttgc
    10441 gatctctgct tcgtcatcgg cacttcgtcg ctcgtctatc cggcgatgcg cctgccggag
    10501 atcgcgctcg aagggggcgc gtacacggtt gagatcaatc ccgaagcgac gccgttctcc
    10561 gcgctctcgg acgtggcgat tcaaggacgc gcgggcgagg tgctgccccg tttggaattt
    10621 tagatgcgga cactctattt cgattgcttt gccggagcga gcggcgacat gatcctcggc
    10681 gcactcgtcg ccgccggagc cgacgcacgc gcgctcactg aacaactcgc gctgctcgat
    10741 gtcgcagact ttgaagtcgc cttcgagaag gttgatcgtt cgggcatcag cgcgacgcat
    10801 gcgcgcgtgc agaccgtcga agaacaacat caccggcacc tcagcgcgat tctcaagatc
    10861 atcgacggtt cgcgtctgag cgaggcggtg aaggctcgcg ccgcgcgcat cttcgagcgg
    10921 ctcggcgagg ccgaagcgcg ggtgcataat gttccggtcg agcgtataca cttccacgag
    10981 gtcggcgcga tggatgccat cattgatgtc gtcggcgcgt gcatcggctt tgaactgctc
    11041 ggcattgaac gcttcgtcgc gtcgccgctg cacgtcggga gcggcacggt cgagatggcg
    11101 cacgggcgtt tccctgtgcc cccgcccgcc gtcttggaac tgctgagcga tgcgcccatc
    11161 tatgcgaccg acatcaaggg cgaactcgtc acgccgacgg gcgcggccat tatctcgacc
    11221 gtcagcgcgg agtttggcgc gctgccacgg atgcgcgtcg cggccacggg ctacggggct
    11281 ggaacgcgcg agtataaaga tttcccgaac gtgctgcgcg tgataatcgg cgagacggaa
    11341 gctgtagaaa ccgctgtcga aaaactttcg cggcgcgaag agttgatcat gatcgagacg
    11401 aacctcgacg acacgacgcc gcaggttctc ggttatgtct cggagcgtgc cttcgcgctc
    11461 ggcgcgctcg attgtttcct gacgccggta cagatgaaga agaatcgtcc gggcgtgctc
    11521 gtctcgattc tgtgccgtcc tgccgcgcag gaggcgttga cgcagttgct ctttgcggag
    11581 acgacgacgc tcggcgtaag gagttacgaa gtcgagcgac gcgcgctcgc cagagagttc
    11641 gtcagcgtcg aaacggagtt cgggaggatc agtgttaagg tggcacgcgg gggagacggg
    11701 cgtgtgagca acgttgcgcc ggagtttgaa gattgccgtg cggcggctct cggcgcgggc
    11761 gtgccgctgc atctggtgga agcggcggcg cgcgcggcat tcgacgcgcg gcgggcggat
    11821 gagaagggaa aagatgaaag cgaaagaggt tgatctgaag aagatcggcg cgggcgtcag
    11881 gctcattcta gagggcatcg gggaagaccc ggagcgggcg ggtctgcaac gcacgccgca
    11941 gcgcgtcgcg gagatgtacg cggaactgac cgccgggatg cgcgaagacc cgcgcgagca
    12001 cgtcacgccg ctgccgggcg attcgcacga cgaactcgtc atcgtcaaag acattcacgt
    12061 cgcttcgatc tgtgaacatc atctcgcgcc gttcgtcggt cgttgtcaca tcgcttacat
    12121 cccgaaaggc gggcgcatcc tcggcctgtc gaaactggcg cggctggccg acacgttcgc
    12181 gcatcgcttg caggtgcagg aacgtctcac ctccgagatc gccgacacga tcttcgacgg
    12241 actcaaaccg ctcggcgtga tggtcgtcat cgaagccgaa cacacttgca tgacgctgcg
    12301 cggcgtcaag aaacccggcg cgctcacgat cacctccgcc attcgcggcg cgtttcgcaa
    12361 agacccgcgc acgcgcgccg aagccatgtc gttgattcaa ggacacagat cgtaaatcgt
    12421 gaatcgtgaa tagagaggaa caggttttct ctattcacga ttcacgattc acgatttacg
    12481 atgtaccggt tatgaaaact ttctacatca cgacgccgat ctattacgcc aacagtctgc
    12541 cgcatctggg gcatctctac acgacgatgg tggccgattg tgtggcgcgc taccggcggc
    12601 agcgcggcta cgaaacctat ttcctgacgg gcacggacga gcacggcatc aacgtgcagc
    12661 gcgccgccgc gcggagcggg cgcacgccga aagagcatgt cgattacatc gtcggcgagt
    12721 tgaaggaaag tttcgccgcc ttcggactcg acggcgagca cggcggctac gacatcttca
    12781 tgcgcacgac agagcctttt cactacgagg ccgtcgcaga gttctggcgt cgcgtcgcgc
    12841 gggcgaaaac tccggcaggg cgcgaagcga tctacaaagg ccactatgaa ggctggttct
    12901 gcgcgccgtg cgcgacgttc aagaccgaag acgaatacac gaagccggag cgcgaaggcg
    12961 acgcgccgct ctgcctgatt cacgaagccc cgctcgaccg cgtggccgag gagagctatt
    13021 tcttccggct ctccgattac gcggaagcgt tgctcgcgct ctacgagtcg cggcctgatc
    13081 tcatccgccc ggaagcgcgg cgcaacgagg tcgtcagttt cgtcaaacgc gggctgcaag
    13141 acctctccgt cagtcgtctc aaaacttcgg tcagttgggc gatccccgtg ccggacgatc
    13201 cggcgcacac gatctacatc tggctcgacg cgctctcgaa ctacatcacc gcgctcggtt
    13261 ggggaagcgg agggggcggg cgcgaaaaca gcttcgataa attctggcct gccgtccacc
    13321 tgatcggcaa agacatcctg cgccagcacg cgatctactg gcctgcgttt ctcatggcgg
    13381 cgggcatcga gcaaccgcgt ctggtctacg cgcacggcat ctggctcgat catcagggtc
    13441 gaaaaatgtc taagacgctc ggcaactcga ttgaactgcc aacgctgcgt cgccattttg
    13501 cgacggacat cgtgcgctac ttctgcctgc gcgagatggc cttcgggcag gacagcaagt
    13561 tcggttacga ggccgtcatc gaacgctcga acagcgatct ggcgagcggc ctcggcaatt
    13621 tgtcgagccg cactctgacg atgatcgggc gttactgcga cggtctgatt cccgcgcccg
    13681 acatcgacga agagaaacgc ttgcaggcca agcgcgcggg cgtggacgca gacggcgggg
    13741 aactcgccag cgcgctcgaa catgcgcgcg attccttcgt gcagcatttc gaggactatg
    13801 ctttcgcgcg tgcgctcgaa acgatctgga cggtcatcgc acgcgcggac aaaatgatct
    13861 cggacgcgaa gccctgggag ttggcgaaag acgagacgca gcggcagacc ctgcacgcgg
    13921 tcttgtatcg ctcggcggaa acgctgcgct ggctcgccgt gctcctccat tccacgatgc
    13981 cggaggcgac gcgcgccatc tggcggcaac tggggcaaac cgacgacatc gcgaaaaatg
    14041 atccggcaga acttcaatgg ggaggtcttc aagcggggac gcgcatcggt gaagtcgcgc
    14101 ccgtcttccc gcgcattgat aaagcgaaaa tcatgtctga gatagaaaaa gagaacgaag
    14161 cgaaacaggc cgcgtcggct gaaactactg cggcgacgca ggcgggaaca cacgcgaccg
    14221 aggccgacgc cgtgcccggt tcggctaatg ctgcggagac gaaggccgag ccgccgcaac
    14281 gcggcgcgac cgaaggcgat gctgctcccg cgacgcaagc ggctgcgccc gaaggcgtcg
    14341 ccactttgat cggcatcgag gatttcgtca aagtcgagat gcgcgtcggc gaaatcctga
    14401 ccgccgagcg catcccgaag tcggacaaac tgctccgctt cacgattgat ctcggcgaag
    14461 ccgcgccgcg acaggtgctc gcaggcatcg ccgaatacta cgaaccggaa aagctcgtcg
    14521 ggcgcaagat catcgtggtg tcgaatctga aaccgcgcaa gctacgcggc ttcgaatcac
    14581 agggcatggt gctcgccgcc tccatcggcg acgaaggccg ccccgtcctc gccactttca
    14641 acgaagacgt accgaacgga gccagactga aatagaacag tgacgagtga taggtgacga
    14701 gtgacgagtt aaaaagacgt gaatcgtcaa tcgtgaatag aagcacccgg tcttaaatct
    14761 attcacgatt cacaatttac gattcacgat tcacttgtct ttaacttgtc actcgtcacc
    14821 tgtcacttgt cactgccttc ttatgtttat tgattcacac gcgcacatag acggggcgga
    14881 gttcgatgcg gatcgcgaag aggtgttggc gcgtgcgcgc gaggcgggcg tcgtgtcgat
    14941 cttgaacgtc ggcacgggcg acccgcacgg cggcgagttg gagcgtgcgg ttgcggtcgc
    15001 cgaacgatac gaaggtgtgt atgcggcggt cggcgtgcac ccgcacgatg cgcgcctgtt
    15061 cgacgacgct gcggcagagc gcgtctacga actcgcgcgg agccgcgagc gcgtgatcgc
    15121 ttggggcgag atcgggctgg attatcatta cgacaactcg ccgcgtgagg tgcagcgcga
    15181 agtcttcgcg cgacagttgc gtctggcgcg tgagttgaaa ctgccgtgca tcatccactc
    15241 gcgcgaggcg gacgaagaca cggtggcgac gctgcgcgcg gagtggagcg gcgcggcgca
    15301 gggcggcatc atgcactgtt tcggcggcgg ctacgagatg gccgcgagcg tgttggagtt
    15361 gggttttatg atctcgttcg cgggcaatgt gaccttcaag aaggccgaag ccttgcgcga
    15421 agtggcgcgt cgcgtgccgc tcgaacgcat gttgatagag acggactgcc cgtttctcac
    15481 gcccgtccct tttcgcgggc ggcgcaacga accggcgcgc gtcgtcgaaa ccgcgcgcca
    15541 tctggcggag ttgcgcggcc tcgaagtcgc ggagtttggc cgcatcacca cgagcaactt
    15601 tcagcgattc ttcaatcttc ccggcgcggc gcaatagtta aaacttttcc cgcccacgcc
    15661 ggagctaaaa cttctgcgcg cccgttcgac gcgacagtca cttggccggg gcaactttta
    15721 ctgcgcaact tttactgcct gagcgttaat gttataaatg ctgcggcgcg cgcgtgtgac
    15781 tgaaatcccg gacggctctt gtcttaaact tgtcacacgc cactggtcac tgttctctta
    15841 tggcacaaca aaattcattc gatattgtct cgcaggttga ccgcgcggag atcaccaacg
    15901 cgctcgatca gacgatgaaa gaggtgcggc agcgtttcga tttcaagggc agcgcggcga
    15961 ccgtcgtgtt ggagaaggaa gaactcattc tcaccgccga ggacgagacg aaactgcgga
    16021 acatgaacga cattttgcag cagaaactcg tgcggcgcag cgtgccgctc aaggctttca
    16081 actacggcaa gatcgaggct gcgctcggcg ggacggtgcg ccagcacgtc gcgattcaac
    16141 agggcatccc gaccgacaag gccaaagaga tcgtcaagtt catcaaggac acgaaggcca
    16201 aagtgcaggc cacgattcag ggcgatgtcg tgcgcgtgac gggcaaagat cgagacacgc
    16261 tgcaagacat catcgccaaa ctcaagacca aagattttaa tatcaatatg caatacacga
    16321 actacagatc aaattagtga cgcgcgacgg gtgacaggca gcaggatttg aaacctgtct
    16381 tccgcctgtc acccgtcgcc tgtcacctgt cactttatga gcatctctag tcatctggcc
    16441 gcgattgaag agccgcaagt tgcggcgacg cgcatcttta gtttgatcgc ggcggaactg
    16501 gctgcggtcg aagaagagtt tgaacgacag gcgcgctcga acattcaagt gatcgcttac
    16561 ataggcgact acctgcgcgc ttcgggcggc aaacgcgtgc gccccgcgct caccattctc
    16621 tcgacttatg cggcgggcgg cgacgccgcg cagccgaacg tgatccggct ggcgaccgtc
    16681 atggagtttc tccacacggc gacgctcgta cacgacgaca tcatcgacaa cgcggagacg
    16741 cgccgcaatc gtccctcgat caacagtcgc ttcggcaacc agacggcggt gctcatgggc
    16801 gactggctct acatgtcggc cttcgaagcg agtctggccg agcgttcgct gcccgtgctc
    16861 gacatcctca cggcggtcac gcggcgaatg accgaaggcg agttgttgca gttgacgatg
    16921 ctcgggcgaa gcgatgtcac cgaggaacag tatttcgaca tcctgatgcg caagaccgcc
    16981 tacctgttca gcgcgtgctg cgagatcggg gggatgctcg gcacgagcga cccggagacg
    17041 caacttgccc tgcgcgacta cggcatgaac ctcggcgtgg ctttccaact cgttgacgac
    17101 ctgctcgatt tcaccgccac cgacgacgtg ctcggcaaag ccgcaggcgt cgatctcatc
    17161 gaaggcaagc tcacgctccc gctcatctac ctgttggaat cggacaggga gcaacgcgcc
    17221 gccgtgcagg ccgtgatgcg cgagggcaac tacgagacga cttcgcgcgc ctccctgatg
    17281 cacgcggcgg agggggcggg cgcgattgag cgggcgcgac agcgcgcaca cgatttcgcc
    17341 acggcggcca gaaaatcgct tgacgtgttg ccgcacacca agtaccgtga tgccttgagt
    17401 tcgattccga cttttatcct tgagcgcgac cgctgaacag aattgcggca acgggcagaa
    17461 ggtggaagga tgaaaattaa cgcgactcaa cttcgattca ccgaaccacg tttggatacc
    17521 gaactgccgt cttccgtctt cctcctgcga ccttgttctt atgccggacg ataatctctt
    17581 agccacgctt gagcagagtt tgcgccagac gcgcgccacg cgaaaccgtc tggccgcgcg
    17641 cctcgccgac ctcgaactcg aagccgagaa actgcgcacc gaactcggcg agatggatgc
    17701 catcgccacg cagaccgagg ccgccatgtt tcgcatcctc tcctccgtgc tcaacaccgt
    17761 gcccgcgaag tcgtcgcctt cggacgccga gatcgaagtg gccttgcgcc gcgacgaagc
    17821 gcaggtacgc aacacgcacc cgcccgccta ccagaccgtg ccgcgttcga ccctgcctcc
    17881 cgtgcgcgcc gacgtggaag tgacgagcga gcgttttctc gaccgcacga tcccacaggc
    17941 gaccgccatg ctcctgcgcg aagcagccgc ccccctgcac gtcaacgaaa tttataatcg
    18001 cctgctcgaa ggcgggtttc agttcaccgg ccacaaccct acgatcagca tcgccgtctc
    18061 tctcaatcgc aaccgacgct tccgcaaagt cgcccccggc actttcgatc tcaccattcg
    18121 cgacgccgca caggcttctt gaggttgcgc tcgtcgaaat ttgagcaggc cgtccggcct
    18181 cgtctccgtc gcgccgtgcg ggcgtgtccg gtcgccctct gcacacacac gccactggcc
    18241 gcaaaacaca ccggcaaaga tgaaaggcaa aaagtaagtg agaaaactga agaccggata
    18301 tacgttcgac ccgcacgccg gagcgaccta cagcttcgag cacatgccgc tcgccgacgc
    18361 cgaagagcgc gcccagcaga cggagacgcc gcatattgtc gaggcggtca actggacgaa
    18421 atccgaaggc ggcgaggtgc attacaaccg ctactacgcg cacaacttcg acgaggctct
    18481 cggcctcgcc gtcaggctcc ggatcgggtt tgaagagggc gtggaagagg tcttatccat
    18541 tcgccccgcc accaagagcg aagcggctga cggcggcgtc gcctccgcgc attttctcga
    18601 actgatgccg ctcgctatct ccagcattga taaaaaggaa ggcacaaaaa aatgagtgaa
    18661 gaaaacagac tgatcgcgct cagcacttta ttcgaagacg tttcgagcgt gcgcggcaaa
    18721 atccctattc ccgtcatcaa gatgctcaaa gcgaaggccg gggacatgct cgctttcgag
    18781 cgtcggcctg acggaacggt cgtcgtgcgc aagtcaacag ccgccgagcg caaggcgggt
    18841 ggcggcggcg ggcggaaggg cagcgggaaa aaatgagcgg gacgggcgac gaagagaagt
    18901 tgaacccgct cgaactcgaa cagcggggct tggacgtgcc gggcgacgaa gggctggtgg
    18961 agggcgaacc gggcgcgggc acggacgcca actactccgc cccgccgccc cgcgtcaaaa
    19021 gcagggaagc gggcgacctc gcgggcgcga cctcgacgca cgacggcgcg cccggcaaac
    19081 ttgaacagac cggcggcgac ggcaccgctc cgacccgcgt gaaataagag ccgcgtaaat
    19141 agttgaagcg aagtggcgga aaagagatag ccggaacgag attgcggggg gctaagtaac
    19201 agtcatttta tgcgtgcgat gattcttgcc gccggttacg gcacacggct gtggcctctg
    19261 acgctcgacc gcgccaagcc cgcgctgcct ttcatgggtc gtcctctcgt cggctacgtc
    19321 gcggaatatc tcgcgcgttt cggttgcgac gagatcatcg tcaacctgca ccaccgcccg
    19381 gagtccgtgc gcgccgcgct cggcgacggg agcgacttcg gcgtgcggct cagctacatc
    19441 gaagaaccgg aaatcttagg cacgagcggc gcgattgaca acgcacgcgc ttatctcgaa
    19501 ggcgacacgt tcatcgtcgt caacggcaag atcgccaccg acatagacct cggcgcggcg
    19561 ctcgaaaccc accgccgcga acgcgcgctc gccacgctcg tcctcaggcc aaacgtcgag
    19621 cgcgaacgct acagcatcgt cgaagttgaa ggccacgcgg tgagagggtt cggcggttat
    19681 ccgcacgcgc cgcccgcccg cacgaacgct gcggaattgc agcttggcgt cgcgtcgcgg
    19741 ggcgaagttg agccgggtgc cgggactgcg cctttgatgt tcacgggcat tcaaattctt
    19801 gagccgcgca tcttcgatta catcccgcgc ggcgtcttct cgcattccgt gacggatgtt
    19861 tacctgcccg ccatcgcgcg cggcgaacgc atcgccgccc acgtctcgga gggaatgtgg
    19921 tatgaactct ccaccattca acgctaccgc gacatcagcc tcacgctcat gcgactggag
    19981 gggcgcgaca ttatttccac gggcgcgggc acaatcgtcg aagcgggcgc ggaggtgcat
    20041 gaggcgattc tctgggagaa cgtgcgcatc gaagcgggag cgcgcgtgag ccgtgcgatt
    20101 atcggcgcgg gcgtgcggat cgggcgcggg gaagttgtcg tggacgcggc ggttgtgcgc
    20161 gccgcgctcg tcgaagggga agagcggcct gcgaaggcgc tggcgggcga ggttcgcggc
    20221 gataattttg tcgtcccact cgcgcagtga tacaattcct gcgttcctaa aagtacttaa
    20281 cagcgggcac aaacgagcct ttccgccgag agcgataatc gcctgaccgc gagcgataat
    20341 cgcgccaacg tgagcgttgg aacgctccgg gcgactctcc gtaccgcgtc gaatacccat
    20401 gagccacccg tcgggcatta ctgcgaacgc gtttgtcgcc tccgcgcatg aacgcttaag
    20461 cgactatctc gtgcgcgcca atctggacgc cgggaaaatc ctcgcgctca cgcccgacgc
    20521 ttccacccgc gaatattttc gtatcccctg gaagaagcgc ggctcaaccg ccgtcgccgc
    20581 cgtctatccc gaaccgttcg accccgaaat tcacccgttt ctcgatgtca cgcgcctgtt
    20641 cgtcgccgcg cgcctgcccg tgccggagat tctggacgtt gacgccgttc acggcatcat
    20701 cgtgcaggaa gacttaggcg accagcaact ccgacgcgtc tttgaaaccg tgagcgaaga
    20761 cgagcgcgag gactatctcg aacgcgccat cagcctcatc gccgacattc aggcggccac
    20821 gccgctcgcc ttcgaacgcg attccatcgc cagccgcctc gcctttgacg aagccaaact
    20881 cttctgggaa ctggaatttt ttctcgaaca ctatttcaga agttatcgca aagagagttt
    20941 gaagcacgcg gaggaagccg aactgcgcgc cgaactgaac gatctcgctg cggaactcgc
    21001 agcccgcccg cgcgtcctct gtcatcgcga cttccacacc tcgaacctga tcgtggacag
    21061 ggagaaccgc ctgcggatca tcgatcatca ggatgcgcga atgggtcccg tcagttacga
    21121 tctcgtctcc ctgctgctcg accgtcaaac cggcgtgccc tcgctcgcgg agatacgaga
    21181 gcgcaggctc tattttttgg aggagagacg acagcgcgga ctcgatgcgc tcgacccgga
    21241 cgagttcggg cgggagtttc gcctgatgac ggtgcagcgt tgtttgaagg cggtcgggac
    21301 gttctcttat caaagcggcg tgtgcgggcg cgaagaggtc tacgcacact tcatcaaccc
    21361 tgtgctcagc atcgtcttgc aggctttgga atggctggcg cgttttccct tgctgcgcga
    21421 aacgatccgc gcccgtctcg gcggaaaatg agatcgccga aacttgcacc gggagcttat
    21481 ctcaaacgca ctcttaatcc ggacgtaaat tgaaacgtta aaaatagcaa tggcaactga
    21541 aaacatagag caggaagaac aagcggcgga agctaaaagt ttaccgcagg tttatatcaa
    21601 tcaactgtcg gagcatgcgg gcgcagaggt caccctgcgc gggtggctct acaatcttcg
    21661 ttccagcggc aaacttctgt ttctgcaact acgcgacggg acgggcatcg tgcaatgcat
    21721 cgtcttcaaa aaaggggtcg cggcggaact ctgggacgcg ctcaagagtc tcggacagga
    21781 atccgcgctc aagattcacg gcacggtgcg cgcagacgaa cgcgcgcccg gcgggtacga
    21841 aattgatgtc tccggcgcgg aagtgttgca ggcggcggag ggttacccga tcacgccgaa
    21901 ggagcacggc acagagtttt tgatggacca caggcacgtc tggctgcgct cgcgccgcca
    21961 gcacgcgatc ctcaaagtgc gccatacgat tgtgcgcgcc gtgcgcgact ttctggataa
    22021 cgacaacttc acgctcgccg atgcgcccgt cctgacgcca gccgcctgcg agggcacgac
    22081 gacgctcttc gaagtcgatt atttcaacga cgagaaggct tacctgacgc agtccggcca
    22141 actctacaac gaggcgaccg ccgccgcctt cggcagatca tattgcttcg gccccacctt
    22201 tcgcgccgag cgttcgaaga cgcgccgcca tctcacggag ttctggatgg tcgagccgga
    22261 gatggctttc gccacgctcg acgacgtgat gaatctcgcc gagcgatttc ttgccaacat
    22321 cgccacgcgc gtcctcgaag agaggcagga ggagttgaag gtgttggaac gcgacactgc
    22381 gaaactcgaa ctcatcgtgc ccccctttcc gcgtctccac tacgacgacg ccgtcaagtt
    22441 gcttcacgaa gggtacgagc ggggggaact cgaagcgaag tttgaatggg gcggcgattt
    22501 cggcgcgccc gacgagacgt atatctcgaa gcaattcgag aaacccgtga tgatccatcg
    22561 ttatccggcg gcgatcaaag ccttctacat ggcgcgcgac ccggagcgtg aggagttggc
    22621 gttgggcgtg gacgtgctgg cctcggaagg ttacggcgaa gtgatcggcg ggggcgagcg
    22681 cgccacctcg ctcgaattcc tcgaagcgca gatcaaagcg cacgacctgc cgcgcgaggc
    22741 tttcgagtgg tatctggatt tgcgtcgcta cggcagtgtg ccgcacggtg ggttcggcat
    22801 gggcatcgaa cgctgcaccg cctggatgtg cggcatcgag cacgtacgcg aaaccatccc
    22861 gttcccgcgc atgctctatc gtctcagacc gtaaaggcag tgacgagtga caggtgacag
    22921 gtgacaagtt aaagcagcgt tcctgtcttt aaacttgctt tggttcctgc tcgtcactcg
    22981 tcacttgtca ctcgtcactg aattattatg agtagtacac aagaacgcat cgtgacgatc
    23041 ttcggcggct cgaagtgcgg cgaggattgc gccgagtatg cagaggcgcg gcgcgccgga
    23101 cagcttttgg ccgaggcggg ttttacgatc tgcacgggcg gttatctggg cgtgatggaa
    23161 gcggcgagtc ggggcgcgca cgagcgcggc gggcgcgtgc tcggcatcgt gatgaaccag
    23221 ttcaaaaaag agccgaaccg ttttctgacc gacaaagttg cgacgccgca cttctatgac
    23281 cgcctgcaac acctgatcgt gcgctcggtc ggcttcatcg cgctgcgcgg cgggatgggc
    23341 acggtgacgg aactctcact cgtctggaac aaaattcaaa cgcgcgtcat cgaaccgcgc
    23401 ccgctggtgc tgctgggcga ttgctggccg cccgtcgtcg aggcgtggcg cagacatttg
    23461 atcgtgagcg aagaagacgt gcggatgctc gatctggcgc agacgccgga agaagctgtc
    23521 aggatcatta tcgaaaagtc gaaaggagtc agtgtatgaa ttgcccgaat tgcggcgcga
    23581 gcacgatcag cggcgcacag ttttgcggat cgtgtggaac gcgcatgagt agcgcgcagg
    23641 ccgctccgaa cgtgtggggc ggcggtgccg gaagcagcgg cgatgtcggc ggcggtgcgg
    23701 cgagcggcgg cgtcggcggt ggcggcgtcg gtcatggtat tcatattgat gcggatgcgc
    23761 gcgggcaggg gcgcggctat agttttgaaa tcctgcacca gccttcgttt gcgctcgcgg
    23821 tggttcagtt gcaggcggaa cagtctattc tcgcggaagc gggcgcgatg gtttcgatgt
    23881 ctgcgaacgt cgagttgcag tcgcagatga agggcggatt gatgggcgcg ttgaagcgcg
    23941 cggtgggcgg cgagtcggcc ttcgtctcga cgtttacggc gcggggcggc cccggcgaag
    24001 tcagtttcgc gccgggcgcg ccgggcgaca tcgccgccat cgagatgagc aatcaaatgt
    24061 tcatggtgca gtcgagttcg tatctggcgg gcgatgcgag tttgacggtt gacacaaaat
    24121 ggggcggggc gaaaactttc ttcggcggcg aagggctgtt cgtcatgcag gtgtcgggca
    24181 cgggcttgct gttgctcacc tctttcggcg cgattcaccg ccggacgctc agaccgggcg
    24241 aacgctacgt ggtggacacg gggcatctcg tcgcgtggga agcctcgatg caatacgctc
    24301 tgcgcaaggc cgcttccggt ttcttccgca cgatggtgag cggcgaaggg atcgtggcgg
    24361 agttcacagg tcccggcgaa ctgttgatcc agacacgcaa ccttgcggcc ttcgccgggc
    24421 tgctgaagcc tttcttcccg tcgcagggaa gcagcggcgg cagcggcttc aatatcaatc
    24481 tcggcgggtg aactgtcaca cgcagtttgg agcattatgt cggctttaag tgaccagacg
    24541 aaatcttctt tgattaatat ccaaccggga gaagggcggc gcgtctcgat catcggcgtg
    24601 ccgctcagct tcggggcggg cacggcgggc atggacctcg gcccggcggc gatgcgcgtg
    24661 gcgcgtctca atcaaagact cgctctgctc ggttatgaag tgcgcgatct cggcgacatg
    24721 cgcctggagc atccgaagat gctcgccgcg ccgcacgaca gagcgaaata cctgcgcgag
    24781 gtcggcatcg tctgcgagga actcgcagcg gaagtgcagc gcgtgatgcg cgcgggtgag
    24841 tttcccatcg ttctgggcgg cgaccattcc atcgccgccg gttcgatagc gggtgcggca
    24901 tcgatctttc acgagcggca gcaacctctg gggcttatct ggttcgacgc gcacgccgac
    24961 atgaacacgc cggagacgag cctctccggc cacgtccacg gcatgccgct cgccgcgctt
    25021 ctgggctacg gtgcgccgga gatgacacac atcggaggct tttcgcccaa actcgatccg
    25081 cgttactgcg cgcacatcgg agcgcgcgat ctcgatccag ccgaacgcga tctgatccgc
    25141 caactaggca tacgcttctt cacgatgcgc gaaattgacg aacgcgggat gagcgcgtgc
    25201 gtcgatgaag cgattgcgat agcgaagcgg gcggcgggcg gctactacgt gacgctggat
    25261 gtggacgcgc tcgatccggg cgacgcgccg ggttcgggca cggtcgtacg cggcgggttg
    25321 acctaccgcg agtcgcatct ggcactggaa aagatcgccg aagcgggcgg catgatcgcc
    25381 ttcgaagtgg tcgaaatcaa caccgccctc gacatcaaca accgcacggc ggaactcggc
    25441 gtcgaactca tcctctcggc catgggcaaa acgattctat aacagtgaca agtgacaggt
    25501 gacgagtgac aagagaagaa cgtgaatcgt gaatcgtgaa tagaaagaaa actgcttccc
    25561 tctattcatg attcacgatt cacgtctttt gttttaactt gtcacctgtc acctgtcact
    25621 tgtcactgtc ttttaggagc ttggtttgaa aagaaaaatc agagtcgggg tgatcttcgg
    25681 ggggcgttcg ggcgagcatg aggtgtctct gcgttcgggg cgggcggtga ttgatgcgat
    25741 tgatcaccgg aagtatgaag tgatcccgat tgcgattacg aagacgggcg cgtggctggc
    25801 tccggctgcc gcgatggagt tgctgccgcg tcagacagcc gagttgttgt cgcgcgggat
    25861 gagcgagaac agggaagcgt tgaccatcgt gggcgaccct tcgcactccg gcctcgtcgc
    25921 cgtcggggta gagggcgggc ggcctgcgcc cccggcgctg ccggtggatg tggtcttccc
    25981 cgtgctgcac ggaacttacg gagaggatgg caccattcaa ggtctgctgg agatggccga
    26041 cgtgccttat gtcggctgcg gcgtgctcgc ttcgtcgtgc gggatggaca aggtgacgat
    26101 gaagatgttg tttcgcgagg cgggtttgcc catctgcaag cacctctggt ttctgcgcgc
    26161 ggagtgggag cgcgaacacg ccggcatcct gcgtcgtgtg gcgcgcgaac tcggatttcc
    26221 atgcttcgtc aagcccgcga atttaggttc atccgtcggc atatcgaagg ccacaaaccg
    26281 ccgcacgctc gaagccgcga ttgatctggc cgcgcgttac gaccgcaaaa ttattgtcga
    26341 agaaggactc gacgcgcgcg agattgagtg tgccgtcatc ggcaacgacg acccggaagc
    26401 gagcctgccc ggcgaataca tcgtgcacga ccagcaaggc gcgtttctcg actacacgga
    26461 gaaatacagc aacaccggcc acgtggaatt cgtcgtgccc gcgccgatcc cgaagtctct
    26521 ggcgacgcgc atccggcgca tgtccgtcgc cgccttcaaa tcaattgacg ggacgggact
    26581 cgcccgcgtg gactttttcc tccgccgcga cacgaacgaa ctgctcatca acgaactcaa
    26641 caccaacccc ggcctcaccg aagtctccgg ctacccgaaa ctctgggcgg cctccggcct
    26701 ccccttctca caagtcatag aacgtctcat ccaactcgca ctcgaacgac acgaagacaa
    26761 atcgcgcaac gaaacaagtc tctgaaataa agtgatgagt gatgagtgat gggtgatgag
    26821 t
//
LOCUS       DASWNS010000141         3256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_421511, whole genome shotgun sequence.
ACCESSION   DASWNS010000141 DASWNS010000000
VERSION     DASWNS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421511"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VGB76_04745"
     CDS             <1..299
                     /locus_tag="VGB76_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098244.1"
                     /translation="LELRSNGMDFDPLGRNARTEDLYIQFAVLEPKTAKVKAQGNVHL
                     RPTSSGRIGGIGRSLPRCYPQPTYAAEFAVIEAGIETAERIFRAFSLPIPQPCS"
     gene            385..783
                     /locus_tag="VGB76_04750"
     CDS             385..783
                     /locus_tag="VGB76_04750"
                     /inference="COORDINATES: protein motif:HMM:NF022070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LITAF-like zinc ribbon domain-containing
                     protein"
                     /protein_id="HEY0098245.1"
                     /translation="MSTIICRNCDQPNAEIARICRYCGTALLSANQTSTPRPPDYAPP
                     QSDFGGIPLWAASPPPAPVVPLHQPGTPEGFRCPHCQSTAAPVIAKRIGTAGWIVFFA
                     LLIFCFPLCFIGLFIKEEYRMCSWCRGAIA"
     gene            complement(879..2474)
                     /locus_tag="VGB76_04755"
     CDS             complement(879..2474)
                     /locus_tag="VGB76_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0098246.1"
                     /translation="MKFFDILSLALRNLREARLRAGLTTLGVIVGVSMIVTMMSFGLG
                     LQRNAVQRFRDLDLFNEINVSGRDLMDLVSSAMEGGEPERRRRAPDGGGPPRRGGAPE
                     RVLNDAALAEIGQIPGVAAVEPNVTLSTYVRANKRAQLRQVGGALVPNAATRFKTFLA
                     GRMISSPEADEAVVDETFLRNFGYEKAQDAIGQTLELLAPGGAGGGNAPPAAGGTTGE
                     KKETGTNGGAGEEAGENGAGDEGSAGVEGGAGDDAPLSFFGLPLEAETPEDDPASALA
                     ARTFRIVGVLQTELEGSAQGRSRFRGLMPVSSIYIPLNAAREWSKQYRNSLNEVAFRL
                     ARESGMIKEGEAEGYQMAVVRVSDPDALPNVQKRLNELGFSSFSVVDQLKELRTVFLI
                     LNSALGLLGGISLLVASFGIANTMIMSILERTREIGIMKAIGAEDREIKLIFFVEAGL
                     IGLAGGVLGSLAAWAIAAVANRVAYRFILKPQGVAYVDFFALPPYLWLGAILFAVVIA
                     ILAALYPAARAARIDPVKALRHD"
     gene            complement(2575..>3256)
                     /locus_tag="VGB76_04760"
     CDS             complement(2575..>3256)
                     /locus_tag="VGB76_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0098247.1"
                     /translation="AARERLARDEDSSPVVALNKFIFEELGFTGNQTDYYDPRNSLLS
                     EVLERRTGIPITLSVVYMEIGRRAGLSVEGVGLPGHFIVRAEEATGGAPVLVDPFNGR
                     IVDAEDCQERLDTIYGGQAPLTDAHLRPVSTLEILARLLRNLKGIYAQAGLYRRALSV
                     IERILLVAPDAIEERRDRGALLAQLGRYSEAHVDVQAYLRRAPDAPDAERVREQLKNM
                     QAQMARLN"
BASE COUNT          588 a         1114 c          959 g          595 t
ORIGIN      
        1 ggctcgaatt gagaagcaac gggatggact tcgaccccct cggacgcaac gcccgcaccg
       61 aagatttata catacagttt gccgtgctcg aaccgaagac ggccaaagtg aaagctcagg
      121 gcaacgtaca tttgcgcccg acgagttcgg gtcgcatcgg cggcatcggt cgcagcctcc
      181 cgcgctgcta cccgcagccc acgtacgccg ccgagtttgc ggttatcgaa gccggaatcg
      241 agacggcgga gcgcatcttc cgcgccttct cgctgcccat tccccagccc tgttcctgaa
      301 acagcgcagg gacgcacggc tctctttact cacaacttaa acgccgcttc gcaatttgac
      361 tgcgcagggc tacttcgcaa ttttatgtcc acgatcatct gccgcaactg cgaccaaccg
      421 aacgccgaaa tcgcgcgcat ctgtcgctac tgcgggacgg cgctcctctc cgccaaccaa
      481 acctcaaccc cacgcccgcc cgactatgcg ccgccgcaaa gcgacttcgg agggattcca
      541 ctctgggctg ccagcccgcc gcccgcgccc gtcgtcccgc tccaccagcc gggcacgccc
      601 gagggctttc gctgcccgca ttgccagtca accgccgcgc ccgttatcgc caaacgcatc
      661 ggcaccgccg gttggatcgt cttcttcgcg ctcctcatct tctgcttccc gctctgcttc
      721 atcggcctct tcatcaaaga ggaatatcgc atgtgcagct ggtgtcgcgg cgcaatcgcc
      781 tgagaaagtg aaggcggaag gcggaaggat gaaagaaagc attcaactgt gtgagtgctt
      841 ttacttcatc cttcatcctt ccgccttcat cgtttcgttc agtcgtgtct gagagctttg
      901 acgggatcga tgcgcgcggc gcgggcggcg gggtagaggg cggcgaggat ggcgatgacg
      961 acggcgaaga gaatcgcgcc gagccagagg tagggcggga gcgcgaagaa gtccacgtag
     1021 gccacgccct gcggtttgag gatgaacctg taggccacac ggtttgccac ggcggcaatc
     1081 gcccacgccg cgagcgaacc gagcacgccg cccgccaagc cgatcaagcc cgcctcgacg
     1141 aagaagatca acttgatctc cctgtcctcc gcgccgatgg ctttcatgat gccgatctcg
     1201 cgcgtgcgtt cgagaatgga catgatcatc gtgttggcga tgccgaacga ggcgacgagc
     1261 agggagatgc cgccgagcag tccgagcgcg gagttgagga tgagaaagac cgtccgcagt
     1321 tccttcaatt gatcgacgac ggagaaactg ctgaagccga gttcgttcag acgcttctgc
     1381 acgttcggca aggcgtccgg gtcgctcacg cgcacgacgg ccatctggta gccctcagct
     1441 tcgccctctt taatcatccc gctctcgcgc gccagccgga acgccacctc gttgagcgag
     1501 ttgcgatact gcttcgacca ctcgcgcgca gcgttgagcg gaatgtagat gcttgagacg
     1561 ggcatcaggc cgcgaaagcg actccgcccc tgcgcgctcc cttcgagttc cgtctgcaag
     1621 acgccgacga tgcgaaacgt ccgcgccgcg agcgcgctcg cagggtcgtc ctccggcgtc
     1681 tccgcttcga gcggcaggcc gaagaagctg agcggcgcgt cgtcgcccgc gccaccttca
     1741 acgcccgcgc taccttcatc gcccgcgcca ttctcgcctg cttcctcacc tgcgccgccg
     1801 ttcgtgccag tctctttctt ctcgcccgtc gtgccacctg cggcgggcgg cgcattgccg
     1861 cctccggctc cgcccggcgc gagcagttcc aacgtctggc cgatggcatc ctgagctttt
     1921 tcgtagccga agttgcgcag gaaggtttcg tccaccaccg cctcgtcggc ctccggcgaa
     1981 ctgatcattc ttccggccag aaaagtcttg aaacgtgtcg ccgcgttggg cacgagcgcg
     2041 ccgcccacct gtcgcaactg cgcgcgttta ttcgcccgca cgtaggtgct cagagtgacg
     2101 ttcggctcca ccgccgcgac gcccggaatc tgcccgatct ccgcgagcgc ggcatcgttc
     2161 agcacacgct ccggcgcgcc cccgcgccgg ggtggaccgc cgccatccgg cgcgcgtcta
     2221 cgccgctccg gctcaccgcc ttccatcgca gacgacacca gatccatcag gtcgcgcccg
     2281 ctcacgttga tctcgttaaa gagatcgaga tcgcggaaac gctgcacggc gttgcgctgc
     2341 aagccgaggc cgaacgacat catcgtcacg atcatcgaga cgccgacgat gacgccgagc
     2401 gtcgtcaacc ccgcgcgcag gcgtgcctcg cgcaagttgc gcagcgcgag actcaatatg
     2461 tcgaaaaact tcataacgaa ttagccacag gttccaaagt cgcaagcctc caagtctcaa
     2521 gtctcaagtc aaacaggctt tcagacctgc gccctgagtc ttgagccttg cgacttaatt
     2581 gagtcttgcc atttgcgcct gcatgttctt caactgctcc ctgacgcgtt cggcgtcggg
     2641 cgcgtcgggc gcgcgtctga gataggcttg cacgtcaaca tgcgcctcgg aatagcgacc
     2701 gagttgcgcg aggagcgcgc cacggtcgcg tcgctcttcg atggcgtcgg gcgcgacgag
     2761 caggatgcgt tcgatgacgg agagcgcgcg gcgatagagt cccgcctgcg cgtagatgcc
     2821 cttcagattg cgcaacaggc gcgcgagtat ttcgagcgtc gagacggggc gcaggtgcgc
     2881 gtcagtgagc ggcgcttgcc cgccgtagat cgtgtccaga cgctcctggc agtcttccgc
     2941 atccacgatc ctgccgttga agggatcgac cagcaccggc gcaccgcccg tggcctcttc
     3001 cgcgcggacg atgaagtgac ccggcaatcc gacgccctcg acgctcagac ccgcgcgccg
     3061 cccgatctcc atgtaaacga ccgacagcgt gatcggaatc cccgtgcgcc gttcgagcac
     3121 ttcggacagg aggctgttgc gcggatcata ataatcggtc tggttcccgg tgaagcccaa
     3181 ctcttcgaag atgaatttat tgagcgcgac caccgggctt gagtcctcgt cgcgcgcgag
     3241 gcgttcacgc gccgcg
//
LOCUS       DASWNS010000142        21735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_83702, whole genome shotgun sequence.
ACCESSION   DASWNS010000142 DASWNS010000000
VERSION     DASWNS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 21735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21735
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83702"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..759
                     /locus_tag="VGB76_04765"
     CDS             133..759
                     /locus_tag="VGB76_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0098248.1"
                     /translation="MQALSGTLTIAEQRGARAEKPLMNSPEQLVALARRGDQEAFRHI
                     FDRWARPVVSFVYDMVGDRALAEDLTQETFVRAYKYLDALRDESKFSTWLFSIAKNVA
                     REHLRSAHSRSRKVDLDDETVLELQDERPTPAGALLDKELNGVVRHALGALDEDKRMV
                     FTLKIFQQRSYEEIAEITGFSVPKVKTDLHRARTEMRKRMSPYLGVKQ"
     gene            756..1709
                     /locus_tag="VGB76_04770"
     CDS             756..1709
                     /locus_tag="VGB76_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098249.1"
                     /translation="MKCEECTASALEELLDGELREPRAAELSAHLAACQSCALRYDAM
                     LDEQAIYASYERDVEVTPQLWAGVAARIAATETSVAAPPRNDGAGLFEKVRAWLATAL
                     VAPRFSPVLTAALVLLAIGATATVMKYSNSQTTPPAAVAGGQGANAAPEQKSVVQPAP
                     ETTPGGAPSKSQDEESSVATVRQRDGREKSTPPEPRRKPEAERKSAQPANDEAQLLAA
                     SPPNEQLRRQRVTMRLVREAESKYVAAIKLLSEDVNRKRTQLDPQVAARFDETLATID
                     RSIAETRRTVLKHGNDPVAVQYMLSAYAKKVEVMREMATLD"
     gene            1770..3266
                     /locus_tag="VGB76_04775"
     CDS             1770..3266
                     /locus_tag="VGB76_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098250.1"
                     /translation="MKKKTLTALIVAAAMIHGTALRTTTRAQEPATPAPGGSVSINNQ
                     AGRVQIKLERGGRVAISNRFGRITIMGWDRDTVEATATSDKGAEAVQVEMTADPAAAQ
                     SVLSLNVPGRPGQRGSSFPYVYTNPNINLSPRIYTAPIPPIYPNPNSPYDPNPNPDQD
                     GAATPNPAKAARPANAPKPSQSSKPAQVIKPAKPSNNNDSNVIIVPLPDMSDAPLPEI
                     AEAPQAVIGTHPAQPRQPSVPAAKGGAARGIGAGAGGGFGAAKGGESSNSGISLLVRM
                     PRYAKLEPLEVRSGDVNISDIDGPVLISSGSSNLNINRVGALEVRARAGNINVHGVEG
                     LVYIVATSGTITVRRTKGDVRATSINGNINLHCIKGRVDASNARGGIVLGGVEGDVEA
                     STTDADINFVGAIRPQGRYRLKSMEGRVIMSIPENSPGFTAILDSYNSDVVTTFPIIT
                     ETLAGGSTRVNRRLVVKQGDGAAQITLDSFSQTVHLTKLASPATNACQ"
     gene            3288..5132
                     /locus_tag="VGB76_04780"
     CDS             3288..5132
                     /locus_tag="VGB76_04780"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0098251.1"
                     /translation="MKFKLLSASLCLSAAATCFAFTDGRVAAQQKGEAKARTPEARVA
                     PEARAEQPEWSVAVPVPVVAPLPALDLNIEMPDINVNVPDIDINMGGVWFYDDNERTE
                     REELRQTYQLSPGARVELSNINGSIDIETHEGNAAELRITSYSHDPNPRKLNVEPAAA
                     SLSVRGEPRKGNADSMNFDNTSYRVWLKLPRRVALTVNGVHDNVRVGEIDGSVRVSNV
                     SGSVGVAQASGSAEVSQVSGSVTLNLARLGAGGVRVENVSGKVAVRFMDDVNANLQTN
                     GIKGKVYVELPNVAVEGEMNRADFRARVGTGGAPINISGVSGSVRLARGRTVAEMLGA
                     LKGAERSTTRVETARDLTLHVSNRQVRHAFVEALNGEQSGSVKMTAARALVPYVAEPE
                     VRDAFMKSIETEAGNGVVRMTAVRAISKQYAADKAVRDMLLRVLAAEQNDMVRVNIIS
                     AISKHADDANVQRALSEALRNDRNDIVRMRAASALSKRVEQPEVYELLLQAARNDKKT
                     IVRANALSGLSRRIKERPELRELFAGYLEHESAIMQYKALQGLVELGDAALKTRLVEK
                     AKELIAMQSRRQWNDRMLLDTLLLLRRLDAQEADRVLEQLGSERMKSY"
     gene            5360..6451
                     /locus_tag="VGB76_04785"
     CDS             5360..6451
                     /locus_tag="VGB76_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098252.1"
                     /translation="MLDGETNKLETQVNEARAALDAHVREIIEWHFNPETGCPFWLDY
                     ASRLDWEPRREIKTYADLSRFGFFQDEALRGGPVRRWVPRGYAGRPVYVFETGGSTGV
                     PKSRINIEDFRLDYEAFSHTLPDEAFPKNSDWLAVGPSGPRRLRLAVEHLAQYRGGIC
                     FMVDLDPRWVIKLLKSGQSKQAEVYKQHVVEQALTLLRAHDNIRCLFTTPKLLEAICE
                     KVSLKKLGITGVFCGGTEMTPQFHRFAVEELLDGVHFAPTYGNTLMGLAVGKPRLPQD
                     NYAIIYYPPAPRAMIEVVDPDRPDTLVGYGETGRVRLTTLTREFFMPRFLERDQAERE
                     PPYLPYAWDGVRNVRPFAKLETNVVEGVY"
     gene            6482..6982
                     /locus_tag="VGB76_04790"
     CDS             6482..6982
                     /locus_tag="VGB76_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098253.1"
                     /translation="MSRSELIRREIVEAFGDVPRPDLKGVRPLGCCEEHDPDFDWYRQ
                     HTWQELEQEFSLDYLDRFEFFALHPSAYHYFVPGLLLGMLESIVTGADGYHAWDWVRN
                     LLFYGSRAGSFGEEYLTLFTAPQREAVASLLEFFNESDVERKGYTDKNNRDVERALSE
                     IWRAES"
     gene            7025..8461
                     /locus_tag="VGB76_04795"
     CDS             7025..8461
                     /locus_tag="VGB76_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0098254.1"
                     /translation="MLHIPLLRKGVPYKSLDVVRVVHHRTREPFVEISQANAGLIRRD
                     LADQASARASLAKFSVAELIEMSVRAAEIFAHDELPVGDEWQTPDAYVEQVSATTGLP
                     FVMARRNMEKIRGALAQMESVLRGLTRNLDLEILDRGYGEIEGQSVSFFPRTQALGVV
                     LPSNSPGVHSLWVPAIALKIPLVLKPGGAEPWTPYRIIQSFTKAGVPPEAFGFYPTGH
                     GSAGEILRHTGRGMVFGDVASTSQWQEDGRVEVHGPGYSKVIIGDDLVDDWEKYLDVI
                     AASILDNGGRSCVNASGVWVTRHADEIAEALAERFARIVPRPAADERAELAPFADPKV
                     AARISQIIEQGLQERGARDVTAEKRGGERFRESDGCAYLLPTVVRCESAEHGLANREF
                     LFPFASVVEVGQEELPEILGASLVVTAITRDPELIAKLLSSPHVDRLNLGAIPTNQIS
                     WDQPHEGNLFEHLYARRAFQQAAAASHQ"
     gene            8458..9801
                     /locus_tag="VGB76_04800"
     CDS             8458..9801
                     /locus_tag="VGB76_04800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0098255.1"
                     /translation="MKILYLTAGAANMYCGSCLRDNALATELIREGHDVMLLPVYTPT
                     LTDERNVSRPDKVLFGGISVYLQQHASIFRRTPWLIDKLWDSTPALKLAARRSIPVNP
                     KLLGELTVSMLRGEEGRQRKEFDKMAHWLREQEKPEVVTLPNSLLLGLARPIREILRC
                     PVVCTLQGEDLFLDGLDENYRRVSLELIRASVAHVDAFVAVNESYAEFMSGHLGIPAS
                     KMRVVPLGINLEDYEARNGAAQARLGAPETGKGTRGGEVFTVGYFARIAPEKGLHVLC
                     EAYRHLRETQGAAQKMRLEAAGYLAPEHEDYLRGIERQMNGWGLGAEFHYRGSLGREE
                     KIAFLQNLDVLSVPATYDEPKGISLLEAMACGVPVVQPRRGGFTEIVERTGGGLLVAP
                     DDAEALAAGIQALRQDPAHAFELGARGIRGVREHYSVAGMAERALEVYREVKGKG"
     gene            9886..10557
                     /locus_tag="VGB76_04805"
     CDS             9886..10557
                     /locus_tag="VGB76_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0098256.1"
                     /translation="MLEVNDLSKEYSTPRGELPILSNVSVSLARGDAISIMGPSGSGK
                     SSFLYIVGALEPPTSGTVTLDGQNPFTMPQRELAAFRNRQIGFIFQDHCLLPQLTVLE
                     NVLTPTLVAGTTNGDELARAQALLDTVGLTARSHHRPAELSGGEKQRVALARALIRRP
                     LLLLCDEPTGNLDHNSATGVAELLLELHRQQETILVVVTHSAELAAKFPVQYELLEQN
                     LVRSS"
     gene            10702..14226
                     /locus_tag="VGB76_04810"
     CDS             10702..14226
                     /locus_tag="VGB76_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098257.1"
                     /translation="MTTATLLRRNLSYYWRTNAAVVCGVAIAVAVLAGALLVGDSVRA
                     SLRELVLQRLGRTAYVVSASNFFREELVEELRGHPEFAVASFEGACPLVVFEGVVIDE
                     ASGRRGGGVQVYGVDERFWEFQGLDAGRRALGEREVALSAGLASELGTQPGGEILLRV
                     EKPSAIPVESLHGRKEDVGRTIRLKMRDALAAADLGEFSLRPQQGAVRAVFVPLKRLQ
                     KDAEQEGRVNTILLSTKAGDDASGAGDAAQAGAFAKILREKFTLADIGVKIRTLDAPH
                     ALSLETESAVVSEALRGQAEAAAQAAGVGTSAIFSYLANAIRAGGREIPYSLVTAFDE
                     ERFAALQREATQLSASPIQASPVPATAQASASGSAPILLNDWAARDLGTRVGDAVSLD
                     YYVWREEGRLETRTAEFRLAGVVPMRGLAADRELTPEYPGISGTESLSDWDPPFPLDL
                     SRVRAQDEDYWKEFRTTPKAFIPLARGQELWQTRYGKLTSLRLHPASGVALETARASF
                     EKTLRARVDPLDANFSLYAARAEGVRASRGATDFGEYFLYFSFFLVVSALLLTSLFFK
                     LGIEQRLREIGILQAVGFPAAKIRTLFLGEGVLLAVAGSILGLACALLYGAFVMHGLR
                     TWWVAAVGTRALSLRVSPVSLALGGAGGIVAALLCIWWTLRSLAPLSARELLTGASDT
                     ARARSNGRRAAKARGRLRLQFMSPARRGVPVSLVFAIACAVLGLLLLAAAALKWLGGV
                     AGFFGAGTLFLIALLCFQSAWLGAAQRRTTIQGRGWWPVVRLGFRNATHRPARSVLCI
                     ALIASATFIIVAVDAFRRDAVHAVSPDRESGTGGYTLLAETLLPLAHDPNTEAGREAL
                     NLSAQEETATLANVTFTRFRLRPGDDASCLNLYQPRNPRILAPTSNFIADRRFTFAAS
                     LAATDAERANPWLLLERDWPDGAIPVIADANSLNYVLHRKLGEEIVLNEETGHPLRLR
                     FVAALSDSLFQSELLMSERNFLRAFPAQEGYRFFLLDAAPERTASVAALLESRLSDFG
                     FDATGAGERLAEFHRVENTYLSTFQTLGGLGTILGTLGLATVLLRNVLERRRELALLR
                     AVGYNARHFALMVVAENALLLLCGLFTGLVCALLAIAPAFYARGGRLPTASLALLLSA
                     VVLTGLAASLAATAAALRTPLLPALRAE"
     gene            complement(14290..14718)
                     /locus_tag="VGB76_04815"
     CDS             complement(14290..14718)
                     /locus_tag="VGB76_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0098258.1"
                     /translation="MKNFYLPLVIAVGGNLLYHLSQKSMPKAANPLYMITISYFVGIA
                     ACLLYGFIYPSERSFMETLRESNWAVYAMGLGAAVIELGFLLAYRAGWNISTAAVACS
                     TAVTLMLVPIGLFAYKEHLSLRNVAGLILCMLGLVLVAKK"
     gene            14833..16116
                     /locus_tag="VGB76_04820"
     CDS             14833..16116
                     /locus_tag="VGB76_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0098259.1"
                     /translation="MRRRNSLHSFFWLVVSVTVLSSSALAGNWPQWRGPQLNGTSDER
                     NLPLKWGAAENLSWKLPLPGLSGATPIVWGERIFLNVAEGDELFLWCVDRNKGALMWK
                     KLLGGGNHKERKHNMSSPSPVTDGKNVYVMTGTGILKSFDFQGRELWARDIQKDHGAF
                     GLQWGYASSPLLHEDSLYVQVLHGMKTDDPSYVLRVNKTTGKTLWRVERPTDAIMESP
                     DAYTTPALLRHAGKTEIVITGADVVTGHDPATGKELWRAGGLNPQNAPFYRIVASPVI
                     YDGIIYAPTRIKPLLAFRAGGRGDISKSHLLWSSQSGPDVPTPVTDGKYFYIVGDKGI
                     IWCLDAKTGKEVYGPVRIKPGDYSSSPVLADGKIYATNEEGLTTVIRAGEKFEVLAEN
                     NLNEYCLSSPAVSDGQIFIRTSGHLYCIGKRAAGR"
     gene            16178..16450
                     /locus_tag="VGB76_04825"
     CDS             16178..16450
                     /locus_tag="VGB76_04825"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEY0098260.1"
                     /translation="MLYMVVENFKHGDALPVYRRFRERGRLAPPGLVYISSWVDENFR
                     RCFQLMATDDRKLLDEWIANWSDLVDFEVIPVLTSQEAAEKIAPSL"
     gene            complement(16463..16672)
                     /locus_tag="VGB76_04830"
     CDS             complement(16463..16672)
                     /locus_tag="VGB76_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098261.1"
                     /translation="MPELKPLKRLVGKAHDLRGAHREPKARPGARPAPMRVWIRHRIP
                     ALNVLVRGLLHAAHHDEAPERNPFK"
     gene            complement(16764..17213)
                     /locus_tag="VGB76_04835"
     CDS             complement(16764..17213)
                     /locus_tag="VGB76_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098262.1"
                     /translation="MVWIVFALGAALSWGVYGAMLHQGQVKLGNPMRALLCVGVAYFL
                     IGVLVPAAWLWSQGALNNFNSGGATTATLAGALGAIGAVCIIFAFRTGGLPTYVMPLV
                     FGGAPLVNVLVTLWLHPPKTAINPMLYVGFLLVSVGAGLVLYFKPQS"
     gene            complement(17378..20545)
                     /locus_tag="VGB76_04840"
     CDS             complement(17378..20545)
                     /locus_tag="VGB76_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0098263.1"
                     /translation="MKFSTINFSNKLRLLLLASFVLCLASASFAQQTDATITGEIKDA
                     QGGVLVGVEVTARNLETGFTRTAVSEDEGRFVFPGMPVGTYEIRSARDGFKPFVQERL
                     RLTIGETAVLNITLEVASIDTQVEVTGSAELVNTQTSELSYLVNEDAIRELPLNGRNY
                     TDLALLQPGVISYPHRDGGSAVAHGLATSINGQDPRSNVYLIDGTPQNDFTNGPAGSA
                     AGTVLGIEGIREFRVETNAYSAEFGRNSGGQINAVSKSGTNEYHGSLYEFHRNDNFDA
                     RNFFDTPRKPEFKRNQFGGSIGGPIRRDKTFFFFNYESLREQLGRTISTVVPDLNARN
                     GILPGVPGQITISPAIRPFLDEFPLPNGSNLGGGLAAYNFGFNQQVTQHFFQGRIDHN
                     FNDRNQFFARYTFDDADQALPTDFPQFPRAFLSRNQFFTAEHRFIQSARTLHTLRFGF
                     GRTRIGQNVESNPTQNLTPFIPGRTLIGNIDIGGVPRFGPQSSADLRLTQNVFSFEHG
                     AVHTRGRHLLKFGGLVERYQDNMYNPTFSLGTWTFSNLRNFLSNSPQSFIGLPANGAL
                     DRYWRFTLFAGYVQDAFRLHERLTLNAGLRYETTTMPVDLYGRDSALPSLTDRAPTTG
                     VLYQNPTRKNLSPRVGFAWDVFGNGKTSLRGGYGVYFNTNNQQNLIVTVTNPPATPRI
                     AIGFTPTSPCRPAFPVAPLSCAFANSIRPVQYDLDNPYLNVYNLNVQQELPWQTVVTL
                     GYAGSRGVHLLRSNDVNTAVAQIRPDGTPFYPAGAPRQNTAFSTIELKSSDGNSWYNA
                     MIFEVRKRWNQNFNVQSSYTFSRNIDTTQASTFFSDATNGTTTAFPEFPGFNYNKGLS
                     DYHAKHNWVVNFVWQIPFAKNLDGIAGALLDDWQLAGIGQARSGNPLTVFVQANRSRS
                     LWQPSLGPGIGRDRASIAPGYTYESAVLGHPDQYFDPNAFVLQPAGTLGNTGRGAFIG
                     PNLRTFDLSAMKNIRFGSPLGENTNVQIRVEAFNLFNRANFAPPGLTVFAGAADNEQP
                     LPTFGRIRSTVTSARQIQLGVRVQF"
     gene            20723..20980
                     /locus_tag="VGB76_04845"
     CDS             20723..20980
                     /locus_tag="VGB76_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098264.1"
                     /translation="MKRILLTLVVIALYISHQDIWNWRKVYPMAFGFIPIGLFYHACF
                     VVAASLVLWMLVTYAWPAHLEAEVEGQQGTKAATAKEEDAR"
     gene            20977..>21735
                     /locus_tag="VGB76_04850"
     CDS             20977..>21735
                     /locus_tag="VGB76_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098265.1"
                     /translation="MIPAIVVFVYLAVVVYIGIFAFRKATGKEKAEDYFLANRSLGPF
                     VFLLSLFGTNMTAFAILGSSGHAFNNGIVTFGLMATSSALVIPLTIFLCGTRIWALGK
                     KHGFMTPVQMFRDRWDCGHIGTVIFVVQAALLVPYIIIGVMGGGTTLNVISGGLVPFW
                     FGGAIVALVVMSYVFFGGMRGTAWVNAFQTILFLVFGAIAVIVIGAGMGGFQSAINGL
                     LASPATAPLLTRERISPYYFFSYTFIPLSAIAFPH"
BASE COUNT         4052 a         6616 c         7076 g         3991 t
ORIGIN      
        1 ccccgcccac ggccaatttt ttttcgcccg aatctccttt ccccgcgccc tttttacttt
       61 tatcctcacc cggctgaaac tttttgcacg ccagacgcac taacacactg gaagacggat
      121 gaaacggcgg ctatgcaagc attgagcggg acattgacga ttgcggagca acggggcgcg
      181 cgcgccgaaa agcctttgat gaacagtcct gagcaactcg tggctctggc gcggcgcggc
      241 gaccaggaag ccttccggca catcttcgac aggtgggcgc gccccgtcgt ctccttcgtc
      301 tacgacatgg tgggcgaccg cgcgctggcc gaggatttaa cgcaggaaac tttcgtccgc
      361 gcctacaaat atctggacgc gctgcgcgac gagtcgaagt tttcgacgtg gcttttcagc
      421 atcgccaaaa acgtcgcgcg cgaacacctg cgctccgcgc acagccgcag ccggaaagtc
      481 gacctggacg acgagacggt gcttgagttg caggacgagc gaccgacgcc cgccggtgcg
      541 ctgctcgata aagagttgaa cggcgtcgtg cgccacgcgc tcggcgcgct cgacgaagac
      601 aagcgcatgg tcttcactct gaagattttc cagcagcgca gctacgaaga gatcgcggag
      661 atcacgggtt tttcggttcc gaaagtgaag accgacctgc accgtgcgag gacggagatg
      721 cgcaagcgca tgagtcctta tctgggggtg aagcaatgaa gtgtgaggaa tgtaccgcaa
      781 gcgcgctcga agaattactg gacggcgaac tccgcgagcc gcgcgccgca gagctgagcg
      841 cgcacctcgc cgcctgccaa agttgcgccc tgcgttacga cgcgatgctc gacgagcagg
      901 cgatctacgc gagctatgaa cgcgacgtgg aggtgacgcc gcaattgtgg gcgggcgtcg
      961 cggcgcgcat cgcggccacg gaaacatccg tggccgcgcc gcctcgcaac gacggcgcgg
     1021 gtctgtttga aaaagttcgc gcgtggcttg cgaccgcgct cgtcgccccg cgtttcagcc
     1081 ccgtgctcac ggcggctctc gtgctcttag ccatcggcgc gacggccacc gtgatgaaat
     1141 attcgaactc gcaaacgacg ccgcccgccg ccgtcgcggg cgggcaggga gcaaacgccg
     1201 cgccggaaca gaagagcgtc gtccagcccg cgcccgaaac aactccgggc ggcgcgccaa
     1261 gtaaatcgca ggacgaagaa tcgtctgtgg cgacagtcag gcaacgggac gggcgcgaaa
     1321 aatcgacgcc gcccgagccg cgacgcaagc ccgaagcgga acgcaaatca gcgcagcctg
     1381 caaacgatga ggcgcagctt ctcgccgcca gcccgccgaa cgagcaactc cggcggcagc
     1441 gcgtcaccat gcgactagtc cgcgaagccg agtcgaaata cgtggcggcg atcaagttgt
     1501 tgtccgaaga cgtgaaccgc aagcgcacgc aactcgaccc gcaagtggcc gcgcgcttcg
     1561 acgaaacgct cgccaccatc gaccgctcga ttgcagagac gcgccgcacc gtgctcaaac
     1621 acggcaacga tccggtcgcc gtccaataca tgctctcggc ctacgccaag aaggtcgaag
     1681 tgatgcgcga gatggcgacg ctcgattaaa cgaaatctcg tctccgtcta attcgaaaac
     1741 ttgaactcat ccgtcaatct gaaagtgtta tgaagaagaa aactttaact gccttaatcg
     1801 tcgccgccgc catgattcac ggcacggctc tgcgcacgac gacgcgcgca caggaacctg
     1861 caacgcctgc gccgggcggc tccgtctcga tcaacaatca ggcgggacgc gtgcagatca
     1921 aactcgaacg cggcgggcgc gtcgccatca gcaatcgctt cgggcgcatc acgatcatgg
     1981 gctgggaccg cgacacggtg gaagccactg cgacgagcga caagggcgcg gaagccgtgc
     2041 aggtggagat gactgccgac ccggctgcgg cgcaatccgt cctgtcgctc aacgtgccgg
     2101 ggcgtcccgg ccagcgcggc agttcgttcc cctacgtcta taccaacccg aacatcaacc
     2161 tgtctccgcg catttacacc gccccgattc cgccgatcta cccgaacccg aattcgccct
     2221 acgacccgaa tcccaatccg gatcaggatg gagccgcgac gcccaacccg gcgaaggctg
     2281 ccagacccgc gaacgctccc aaaccctcac aatcttcgaa gcccgcgcag gtcatcaagc
     2341 ccgcgaagcc ttcgaataat aacgacagca acgtgatcat cgtccccctg ccggacatgt
     2401 ccgacgcgcc cctcccggag atcgcggaag ctccgcaggc cgtcatcgga actcaccccg
     2461 cgcagccgcg ccagccttcg gtgccagcgg cgaaaggcgg cgcagcgcgc ggcatcggcg
     2521 cgggagcggg cggaggcttc ggggcagcga agggcggcga gagcagcaac agcggcatca
     2581 gcctgctcgt gcggatgccc cgctacgcca aactcgaacc actcgaagtg cgctccggcg
     2641 atgtcaacat ctcggacatc gacgggccgg tcttgatctc aagcggcagc agcaacctca
     2701 acatcaatcg cgtcggcgcg ctcgaagtgc gtgcgcgtgc gggcaacatc aacgtgcacg
     2761 gcgtggaagg tctggtctat atcgtggcga cgagcggcac gatcaccgtc cgccgcacga
     2821 agggcgacgt gcgcgccact tccatcaacg gcaacatcaa cctccactgc atcaaggggc
     2881 gcgtggatgc ctcgaacgcg cgcggcggca tcgtgctcgg cggcgtcgaa ggcgacgtgg
     2941 aggcttccac cactgacgcc gacatcaact tcgtcggcgc gattcgcccg cagggtcgct
     3001 accgcctgaa atccatggaa ggccgcgtca tcatgtcgat cccggaaaac tcgccgggct
     3061 tcacggcgat tctcgactct tacaacagcg acgtggtgac caccttcccc attatcaccg
     3121 agacgctcgc gggcggcagc acgcgcgtca accgccgcct ggtggtcaag cagggcgacg
     3181 gcgcggcgca gatcacgctc gactccttca gccagaccgt tcacctgacc aaactcgcgt
     3241 cgcccgcgac aaacgcttgc cagtaattcc ataagaggtc atcacagatg aaattcaaac
     3301 tactcagcgc atctctctgt ctctccgccg ccgcaacctg cttcgccttc acggacgggc
     3361 gcgtcgccgc gcagcaaaaa ggggaagcca aagcgagaac gcccgaagcg cgggtcgccc
     3421 ccgaagccag agccgagcaa ccggaatggt ctgtggccgt cccggtgcct gtcgtcgccc
     3481 cgctcccggc tctcgacctc aacatcgaga tgcccgacat caacgtcaac gtgcccgaca
     3541 tagacatcaa catgggcggc gtctggttct acgacgacaa cgaacgcacg gagcgcgaag
     3601 agttgcgaca gacttatcaa ctctcgcccg gcgcacgcgt cgaactctcg aacatcaacg
     3661 gctccattga catcgagacg cacgagggca acgccgcaga gctacgcatc acgagctatt
     3721 cgcacgaccc gaacccgcgc aagctcaacg tcgaaccggc ggctgcgagc ctctccgtgc
     3781 ggggcgagcc gcgcaaaggc aatgccgaca gcatgaactt cgacaatacg agttaccgcg
     3841 tctggttgaa gctgccgcgc cgcgtcgcgc tcaccgtcaa cggcgtgcac gataacgtcc
     3901 gggtcggcga aattgacggt tcggttcgcg tgagcaacgt gagcgggagc gtcggcgtcg
     3961 cgcaggcgtc ggggtctgcg gaagtgtcgc aggtgagcgg cagcgtcacg ctgaatctgg
     4021 cgcgcctcgg cgcgggcggc gtgcgcgtcg agaacgtgag cggcaaagtc gccgtgcgct
     4081 tcatggacga cgtgaacgcc aatttgcaga cgaacggaat caagggcaag gtctatgtcg
     4141 aactgccgaa cgtggcggtc gagggcgaga tgaaccgcgc agactttcgc gccagggtcg
     4201 gcacgggcgg cgcgcccatt aacatctccg gggtatcggg cagcgtgcgg ctcgcgcgcg
     4261 ggcgcacggt ggcggagatg ctcggcgcgt tgaagggcgc ggagcgttcg acgacgcgcg
     4321 tggagacggc gcgcgatctc accttgcacg tctcaaaccg gcaggtgcgc cacgccttcg
     4381 tcgaagccct gaacggcgag cagagcggct ccgtgaagat gaccgccgcc cgcgcgctcg
     4441 tgccttacgt ggccgagccg gaagtgcgcg acgcttttat gaagtccatc gagacggagg
     4501 cgggcaacgg cgtcgtgcgt atgacagccg tgcgcgccat ctccaaacaa tacgcggcgg
     4561 acaaggccgt gcgcgacatg ctgctgcgtg tgctggccgc cgaacagaac gacatggtgc
     4621 gcgtgaacat catcagcgcg atcagcaagc acgcggacga cgccaacgtg cagcgcgccc
     4681 tctcggaagc gttgcgtaat gaccgcaacg acatcgtgcg gatgcgcgcg gcgagcgcac
     4741 tctcgaagcg cgtcgagcag ccggaagttt acgaactgct cttgcaggcc gcgcgcaacg
     4801 acaagaagac catcgtgcgc gccaacgctt tgagcggcct gagccgtcga atcaaagagc
     4861 gcccggagtt gcgcgaactg ttcgccggtt atctcgaaca cgagtcggct atcatgcagt
     4921 acaaagccct gcaaggattg gtcgaactcg gcgacgccgc gctgaaaacc cgcctcgtcg
     4981 agaaagcgaa agagttgatc gcgatgcagt cgcgccgaca gtggaacgac cgcatgctgc
     5041 tcgacacgct gctcctgctc cgacgcctcg acgcgcagga ggccgaccgc gtgctcgaac
     5101 aattggggag cgagcgcatg aagagttatt gagcggagag cgcgtagaga gagaacgtgt
     5161 gctgatcttc ggattccgtc gcggctggca aaacccctcg cccggacaca aagacgcgaa
     5221 gttaaatcat aaaatttact tcgcgtcttt ctctgtttgt ggttgagcgg gtggctgccg
     5281 gtcgcatccg aaaattcgta accgcaaaaa gagcctgtgc gtttgcgctc acgttcgagc
     5341 cgtctgtaga atcgggcgca tgcttgatgg cgaaacgaat aaacttgaga cgcaggtgaa
     5401 cgaagcgcgc gccgcactcg acgcgcacgt gcgcgagatc atcgaatggc atttcaaccc
     5461 tgaaaccggc tgccccttct ggctcgacta cgccagccgt ctcgattggg aaccgcgccg
     5521 cgagatcaag acttacgcag acctctcgcg cttcggtttc tttcaggacg aggctttgcg
     5581 cggcggaccc gtgcgccgct gggtgccgcg cggttacgcc gggcgtcccg tctacgtctt
     5641 cgaaaccggc ggctcgacgg gcgtgccgaa gtcgcgcatc aacatcgaag attttcgtct
     5701 cgactacgaa gcgttcagcc acacgctgcc cgatgaagcc ttcccgaaaa actccgactg
     5761 gctcgccgtc ggcccctccg gaccgcgccg cctgcgtctc gccgtcgagc atctggcgca
     5821 atatcgcggc ggcatctgct tcatggtcga tctcgatccg cgttgggtca ttaaactttt
     5881 aaagagcggc cagtcgaagc aggccgaagt ctacaagcaa cacgtcgtcg aacaggcgct
     5941 cacgctcctg cgcgcgcacg ataacattcg ctgcctcttc accacgccga aactgctgga
     6001 agccatctgc gaaaaagtct ctctgaaaaa actgggcatc accggagtct tctgcggcgg
     6061 cacggagatg accccgcagt ttcaccgctt cgccgtcgaa gaactgctcg acggcgtcca
     6121 cttcgcgccg acctacggga acaccctgat ggggctggcc gtcggcaagc cgcgcctgcc
     6181 gcaagacaac tacgccatca tctactatcc gcccgcccca cgcgccatga tcgaagtggt
     6241 tgatcccgac cgtcccgaca cgctcgtcgg ctacggcgag acggggcgcg tgcgcctgac
     6301 gactttgacg cgcgagttct tcatgccgcg cttcctcgaa cgcgatcagg ccgaacgcga
     6361 gccgccctac ctgccatacg cgtgggacgg cgtgcgcaac gtccgaccct tcgccaagct
     6421 ggaaacgaac gtggtggaag gcgtctactg agtcctacgg ttcccgccgc gagggtgaag
     6481 agtgagccgg agtgaattga tcagacggga aatagttgaa gccttcggcg atgttccgcg
     6541 tcccgatttg aaaggcgtcc gcccgctggg gtgttgcgaa gagcatgacc cggatttcga
     6601 ctggtatcgt caacacacct ggcaggagct tgagcaggag ttttcgctcg actatctgga
     6661 caggttcgag ttcttcgcgc tgcacccgtc cgcctatcac tacttcgtgc cgggtctgct
     6721 cctggggatg ctggagtcaa tcgttacggg tgcggacggt tatcatgcct gggattgggt
     6781 gcgcaacctg ctcttctacg ggagccgcgc cgggagtttc ggggaggaat acctgaccct
     6841 gttcaccgcg ccgcagaggg aggcggtcgc gagcctgctt gagtttttca acgaatcgga
     6901 cgtggagcga aagggctaca ccgacaagaa taatagagat gtggagcgcg ctctaagtga
     6961 gatatggcgc gctgaatcct gattgagaga tgagccgggc gcgccttcaa gcgtccattt
     7021 aattatgttg cacataccat tgttgcgcaa aggcgtgccc tataaaagtc tggacgtggt
     7081 gcgcgtcgtg catcaccgga cgcgtgagcc gttcgtggag atcagtcagg cgaacgcggg
     7141 actcattcgt cgtgatctgg cagatcaagc gagcgcgcgt gcgtcgctgg caaaattcag
     7201 cgtcgccgaa cttattgaga tgtctgtgcg tgcggcggag atttttgcgc acgacgaact
     7261 cccggtcggc gatgaatggc agacgccgga cgcttacgtc gagcaggtgt cggcgacgac
     7321 ggggctgccc tttgtgatgg cgcggcgcaa catggagaag attcgcggcg cgctcgcgca
     7381 gatggagagc gtgctcagag gtttgacgcg caatctcgat ctcgaaatct tggatcgcgg
     7441 gtacggcgag atcgaagggc agtccgtgag tttcttcccg cgcacgcagg cgttgggcgt
     7501 ggtcttgccg agcaattcgc cgggcgtgca ttcgctgtgg gttccggcta tcgcgctcaa
     7561 gattccgctc gtcctcaagc cgggcggcgc ggaaccctgg acgccgtatc gaatcattca
     7621 gtcgttcacg aaggcgggcg tgccgccgga agcgttcggg ttttacccga ccggtcacgg
     7681 cagcgcgggg gagatactgc gccacacggg gcgcggcatg gtcttcggcg acgtggcctc
     7741 cacttcgcaa tggcaggagg acgggcgcgt ggaagtgcac ggccccggct acagcaaagt
     7801 gatcatcggc gacgatctgg ttgacgattg ggaaaaatat ttagacgtga tcgcggcttc
     7861 gattctggat aacggcgggc gttcgtgcgt caacgcgtcg ggcgtgtggg tgacgagaca
     7921 tgcggacgag atcgcggaag cactggccga acgattcgcg cgcatcgtgc cgcgccccgc
     7981 cgccgacgag cgggcggaac tcgcgccctt tgccgacccg aaagtggccg cgcgcatttc
     8041 acagatcatc gaacagggtt tgcaggagcg cggcgcacgc gacgtgacgg cggagaagcg
     8101 cggcggcgag cgattccggg agtcggacgg ttgcgcttac ctgttgccga ccgtggtgcg
     8161 ctgcgagtcg gcggagcatg ggctggcgaa ccgcgagttt ttgtttccgt ttgcgagcgt
     8221 ggtcgaagtc gggcaggaag agttgccgga aattctgggt gcgagcctcg tggtgacggc
     8281 gatcacgcgc gacccggagt taatcgcgaa acttttatcg tcgccacacg ttgatcgtct
     8341 caacctcggc gcgatcccga cgaaccagat cagttgggac cagccgcacg agggaaattt
     8401 gttcgagcat ctctacgcgc gcagggcttt ccagcaagcg gcagccgcgt cgcaccaatg
     8461 aagattctct atctcaccgc cggggctgcc aacatgtact gcggcagttg cctgcgcgac
     8521 aacgcgctcg ccaccgaact gatcagagag gggcacgacg tgatgctcct gcccgtctat
     8581 acgccgaccc tgaccgacga gcgaaacgtc agccgcccgg ataaagttct cttcggcggc
     8641 atcagcgtct atctccaaca gcacgcctcc atctttcgcc gcacgccctg gctcatagac
     8701 aaattgtggg actcgacccc ggctctcaaa ctcgccgcgc gccgctcgat tccggtcaac
     8761 ccgaaactgc tgggcgaact caccgtctcg atgctgcgcg gcgaagaggg acgccagcgc
     8821 aaagagttcg acaagatggc gcactggttg cgcgagcagg agaagccgga ggtggtgacg
     8881 ctgcccaatt cgctcctgct cggcctcgcg cgtcccatca gagagatttt gcgctgcccc
     8941 gtcgtctgca cgctgcaagg cgaagacctg tttctagacg gactcgacga gaattatcgt
     9001 cgcgtgtcgc tcgaactcat ccgcgcgagc gtcgcgcacg tagatgcctt cgtcgcggtc
     9061 aacgagtctt acgcggagtt catgtcaggg catctgggga tacccgcgag caagatgcgc
     9121 gtcgttccgc tcggcatcaa tctcgaagat tacgaagcgc gaaacggggc ggcgcaggca
     9181 cgactgggcg cgccggagac gggcaaggga acgaggggcg gcgaagtgtt caccgtcggc
     9241 tactttgcgc ggattgcgcc ggagaaggga ttgcatgttt tgtgcgaggc gtaccggcac
     9301 ctgcgcgaga cgcagggcgc ggcgcagaaa atgcggctcg aagcggcggg ctatctcgcg
     9361 ccggagcacg aagattattt gcgcggcatc gagcggcaga tgaacgggtg gggactcggt
     9421 gcggagtttc attatcgcgg ctcgctcggg cgcgaagaga agatcgcgtt tctgcaaaat
     9481 ctcgacgtgc tctccgtgcc cgcaacctac gacgaaccga agggcatctc gttgctcgaa
     9541 gcgatggcct gcggcgtgcc cgtcgtccaa ccgcgacgcg gcggcttcac cgagatcgtc
     9601 gagcgcacgg gcggcggcct gctcgtcgcg ccggacgatg ccgaagcctt agccgcaggg
     9661 attcaagccc tgcggcaaga ccccgcgcac gcctttgaac tcggcgcacg cggcatacgc
     9721 ggagtgcgcg aacattacag cgttgcgggc atggccgaac gcgcgctcga agtctaccgg
     9781 gaggtaaagg ggaaggggta aagggtaaag gtaaaatggc ggaaaacagt tttcaccatc
     9841 ctttccccct ttccctttcc tccttcccac cggctgttcc aacttatgct tgaagtcaac
     9901 gatctctcca aagaatattc gacgccgcgc ggcgagttgc cgatcctgtc gaacgtctcc
     9961 gtgagcctgg cgcgcggcga tgcgatctcg atcatgggtc cttcggggag cggcaagagc
    10021 agctttctct acatcgtcgg cgcgcttgag ccgcccactt ccggcacggt cacgctcgac
    10081 ggccagaacc ctttcacgat gccgcaacgc gaactcgccg cttttcgcaa ccgccagatc
    10141 ggtttcatct ttcaagacca ctgtttgctg ccgcaattaa ccgtactgga aaacgtgttg
    10201 acgccaacgc tcgtcgcggg cacgacgaac ggcgacgagt tggcgcgggc gcaagcgttg
    10261 ctcgacacgg tcggactcac ggcgcgcagc catcatcgcc cggcggaact ctcgggcggc
    10321 gagaagcagc gcgtcgcgct cgcacgcgcg ctcatacgcc gcccgctcct cctactctgc
    10381 gacgagccga ccggaaacct cgatcacaac tccgccacgg gcgtcgccga acttctactc
    10441 gaactgcacc ggcagcagga gacgatcctc gtcgtcgtta cgcacagcgc ggagttggcc
    10501 gcgaagtttc cggtgcaata tgaactgttg gagcagaatc ttgtgcgtag ttcctagttc
    10561 acagtcagct tgtgagttgt ccattgtcca ttgtcagtag cccgtcgtcc gttgtttgtg
    10621 attcataagc cgcgcgctca ggacgaaggc tcaagttagt caacgcgaaa acaggtgaca
    10681 gctaatcgct tacagtctct tatgacgacc gccacgctgc tcagacgcaa tctctcttac
    10741 tactggcgca cgaacgcggc ggtggtgtgc ggcgtggcga ttgccgtggc cgtgctggcg
    10801 ggcgcgttgc tcgtcggcga ttcggtgcgc gcgagcttgc gcgaactcgt gttgcagcgt
    10861 ctcggtcgga cggcctacgt cgtctcggcg tcgaatttct tccgcgaaga gctggtcgaa
    10921 gagttgcgcg ggcatccgga gtttgccgtt gcctcgttcg agggggcgtg cccgctcgtg
    10981 gtgtttgaag gcgtcgtgat tgacgaggcg agcgggcggc ggggcggggg cgtgcaggtc
    11041 tacggcgttg acgaacgctt ctgggaattt caagggctgg acgcagggcg gcgcgctttg
    11101 ggcgagcgcg aagttgctct gagcgcgggg ctggcctcgg agttgggcac gcagccgggc
    11161 ggcgaaatct tgttgcgcgt agaaaagcct tcggccatcc cggtcgaatc gctgcacggg
    11221 cgcaaggaag acgtggggcg cacgattcgc ctgaagatgc gcgacgcgct cgccgccgca
    11281 gacctcggag agtttagcct gcgaccgcag cagggcgcgg tgcgcgcggt cttcgtcccg
    11341 ctcaagcgtc tgcaaaaaga tgcggagcag gaggggcggg tcaacacgat cctgctctcg
    11401 acgaaggcgg gcgacgacgc gagcggcgca ggcgacgcgg cgcaggccgg ggcgtttgcg
    11461 aagattctgc gcgagaagtt tacgctcgca gacatcggcg tcaagattcg cacgctcgac
    11521 gcgccgcacg cgctgtcgct cgaaaccgag agcgcagtgg tgagcgaagc gttgcgcggg
    11581 caggcggaag cggctgctca agcggcgggc gtcggcacgt ccgcgatctt ctcttatctc
    11641 gccaacgcga tccgcgcggg cgggcgcgag attccttatt cgctcgtcac ggcgtttgac
    11701 gaagagcgtt tcgcggcgtt gcagcgggag gcgactcaac tctcggcgtc gcccattcaa
    11761 gcgtcgcctg ttccggcgac ggctcaagcg tctgcgagtg ggagcgcgcc gatcctgttg
    11821 aacgattggg cggcgcgcga tctcgggacg cgtgtgggcg atgcggtgtc gctcgattat
    11881 tacgtgtggc gcgaagaggg gcgtctggag acgcgcacgg cggagtttcg tctggcgggc
    11941 gtcgtgccga tgcgcgggct ggcggcagac cgcgagttga cgccggagta tccgggcatc
    12001 agcggcacgg aaagtctgtc ggattgggac ccgccgtttc cgctcgacct ctcgcgcgtg
    12061 cgcgcgcagg acgaggacta ctggaaagag tttcgcacga cgccgaaagc cttcatccca
    12121 ctcgcgcgcg ggcaggaact ctggcagacg cgctacggca agttaacttc gctgcgcctt
    12181 catcccgcga gcggcgtcgc gctcgaaacg gcgcgcgcga gttttgagaa gactttgcgg
    12241 gcgcgcgtgg acccgcttga cgcaaacttt tctctctacg cagcacgcgc cgaaggagtc
    12301 cgagcctcgc gcggcgcgac cgacttcggc gagtattttc tctacttcag tttcttcctc
    12361 gtcgtctccg cgctgctgct cacgtcgctc ttcttcaaac tcggcatcga gcaacgcctg
    12421 cgcgagatcg gcatcctgca agcggtcggg tttcccgccg ccaaaattcg cacgctcttt
    12481 ctcggcgaag gcgtgctgct tgccgtcgcg ggcagcatcc tcggactcgc gtgcgcgctg
    12541 ttgtacggtg cgttcgtgat gcacggcctg cgtacgtggt gggtggctgc ggtgggcacg
    12601 cgtgccctgt cgttgcgcgt ctcgcccgtg tcgctcgctt taggcggggc gggcggcatc
    12661 gtcgccgcgc tgctgtgcat ctggtggacg ctcagatcgc tcgcgcctct ctcggcgcgc
    12721 gaactgttga ccggagcgag cgacaccgca cgagccaggt cgaacgggcg acgcgctgcg
    12781 aaggcgcggg gcaggctccg cttacagttc atgtcgcccg cgcggcgcgg cgtgccggtg
    12841 tcgcttgtgt tcgccatcgc gtgcgcggtt ctcggcctgt tgctgctcgc cgccgccgcg
    12901 ctcaagtggc tcggaggggt ggccggattc ttcggcgcgg gcactctgtt tctcatcgcg
    12961 ctcctctgct ttcaatccgc ctggctcggc gcagcgcagc ggcgcacgac gattcagggg
    13021 cgcggttggt ggccggtcgt gcgtctcggc tttcgcaacg cgacgcaccg ccccgcgaga
    13081 agcgtgctct gcatcgcgct catcgcctcc gccacgttca tcatcgtcgc ggttgacgcc
    13141 ttcaggcgcg acgccgtaca cgccgtctct ccggacagag aatccggcac gggcggttac
    13201 acgctcctcg ccgagacgct cctgccgctc gcacacgatc cgaatacgga agcggggcgc
    13261 gaggctctga acctcagcgc gcaggaggag acggcgacgc tcgccaatgt aaccttcacg
    13321 cgctttcgtc tccgaccggg cgacgatgcc agttgtctca atctctacca gccgcgcaac
    13381 ccgcgcatcc tcgcgccaac ttccaacttc atcgcggaca gacgcttcac cttcgccgct
    13441 tcgctcgccg cgaccgacgc ggagcgagcc aacccgtggc tgctgctcga aagagattgg
    13501 ccggacggcg caatccccgt catcgccgat gccaactctt tgaactacgt gctgcatcgc
    13561 aaactcggcg aagagatcgt gttgaacgaa gagacgggac atccgttgcg gctgcgcttc
    13621 gtcgccgcgc tctcggacag cctgttccag tcggagttgt tgatgtcgga gcgaaatttc
    13681 ctgcgcgctt ttcccgcaca ggagggctac aggtttttcc tgttggacgc cgcgccggag
    13741 agaacggcga gcgtcgccgc gctgctcgaa tcgcgcctgt cggacttcgg cttcgacgcg
    13801 acgggggcgg gcgagcggct cgcggagttt catcgcgtgg agaacaccta tctctcgacc
    13861 ttccagacgc tcggcgggct tggcacgatt ctcggcacgc tcggcctggc gaccgtctta
    13921 ttgcgcaacg tccttgagcg gcgacgcgaa ctcgcgctgc tgcgcgccgt cggctacaac
    13981 gcgcgccact tcgccctgat ggtcgtcgcg gagaacgcgc tgctgctcct ttgcggtctg
    14041 ttcacggggc tggtctgcgc gctgctcgcc atcgccccgg ccttctacgc gcgcggtggc
    14101 cgtctcccca ccgcttcgct cgcgctcttg ctctcggcgg tcgtcttaac cggactcgcc
    14161 gcgtcgctcg cggcaactgc ggctgcgctg cgcacgcccc tgctcccggc cttgcgcgcc
    14221 gaataagaga gggcgcaact gcgtcgcaca cgggcgcgca ggcgcagtct gccgcgaaca
    14281 gatggcgcgc tactttttcg ccacgaggac gaggccgagc atgcagagga tcagcccggc
    14341 gacgttgcgc agcgagagat gttctttgta agcgaacagg ccgatgggca ccagcatcag
    14401 cgtcacggca gtgctgcacg caaccgccgc cgtgctgatg ttccacccgg cgcgataggc
    14461 cagcaggaag ccgagttcga tgaccgccgc gcccaagccc atcgcgtaga ccgcccagtt
    14521 cgactcgcgc agcgtttcca tgaacgacct ctcgctcggg tagatgaacc cgtagagcag
    14581 gcacgcggca atgccgacga aataggagat ggtgatcatg tagagcgggt tggccgcctt
    14641 cggcatggac ttctgcgaga ggtgatacag cagattgccg ccgacggcaa taacgagtgg
    14701 caggtaaaag tttttcatct gttagttgaa attggcgcgg gcaatgcatc gcgtcgccgt
    14761 gcggtaaatt ttgtgatctg tcttgcccaa gatgggtcta tattaagtcg gtttcactgg
    14821 aggaggaaat tcatgcgccg tcgaaactct ctgcacagct tcttttggct cgtcgtgtcg
    14881 gtaacggttc tctcaagcag tgcgctcgcg ggcaactggc cgcagtggcg cggtccacag
    14941 ttgaacggca cgagcgacga gcgcaacttg ccgctcaagt ggggcgcggc ggagaacctc
    15001 agttggaaac tgccgctgcc cggattgagc ggggcgacgc ccatcgtgtg gggcgagcgc
    15061 atttttctga acgtcgcgga gggcgacgaa ctgttcctct ggtgcgtgga caggaacaag
    15121 ggcgcgctca tgtggaagaa gttgcttggc ggcggcaacc ataaagagcg caagcacaac
    15181 atgtcttcgc cctcgcccgt gaccgacggc aagaacgtct acgtgatgac gggcacgggc
    15241 atcctcaaga gcttcgactt tcagggacgc gaactctggg cgcgcgacat ccagaaagat
    15301 cacggcgcgt tcggcttgca gtggggctac gcctcctcgc cgctcctgca tgaagattcg
    15361 ctctacgtgc aagtgcttca cggcatgaag acggacgatc cttcctacgt gctgcgcgtc
    15421 aacaagacga cgggcaagac cctctggcgc gtggaacgcc cgaccgacgc catcatggaa
    15481 tcgcccgacg cctacacgac gcccgcgctc ctgcgtcacg cgggcaagac cgagatcgtc
    15541 atcaccggcg cggacgtggt aacagggcac gaccccgcca ccggcaaaga actctggcgc
    15601 gcgggcggcc tcaatccgca aaacgccccc ttctatcgca tcgtcgcctc gcccgtcatc
    15661 tacgacggca tcatctacgc gcccacacgt atcaagccgc tcctcgcatt tcgcgcgggc
    15721 gggcgcggcg acatctcgaa gtcacacctg ctctggtcgt cgcagtcagg accggacgtg
    15781 ccaacgcccg tgacggacgg gaaatatttc tacatcgtcg gcgacaaagg catcatctgg
    15841 tgtctcgacg cgaagaccgg aaaagaagtt tacggtcccg tccgcatcaa gccgggcgac
    15901 tattcctctt cgcccgttct ggccgacggc aagatttacg cgacgaacga agaaggtttg
    15961 acgacggtta ttcgcgcggg cgagaagttc gaagtgctcg cggaaaataa cctgaacgaa
    16021 tactgtctca gttcgcccgc cgtctccgac gggcaaattt tcatacgcac ctccggccat
    16081 ctctactgca tcggcaaacg cgcggcgggg cgttgaaagc tttcggtttt cgggtgaacc
    16141 ggcgggtagc ggtcttgaac cagagcggcg tgcagcgatg ctgtatatgg ttgtcgaaaa
    16201 ctttaagcac ggagatgcgt tgcccgtcta tcgacgcttc cgggagcggg ggcggctcgc
    16261 tcccccgggg ttggtctata tttcgagttg ggttgacgag aacttccgac gctgctttca
    16321 actgatggcg accgatgatc gaaagctgct cgacgaatgg atcgctaact ggagcgacct
    16381 cgtcgacttc gaggtcattc cggtgctgac ctcgcaggag gctgcggaaa agatcgcccc
    16441 gtctctctag atcgagagag cgttatttga aggggttgcg ttcgggggct tcgtcatgat
    16501 gcgcggcgtg gagaagtccg cgcacgagga cgttgagggc ggggatgcga tgcctgatcc
    16561 agacgcgcat gggagcggga cgcgcgccgg gtcgtgcctt cggttcgcgg tgcgccccgc
    16621 gcaggtcgtg cgccttgccg accagtctct tgaggggctt gagttcaggc ataaaaaaag
    16681 ggcggcgacc ggaatcggtg aagcgttagc gaagcgtcgg gcaagcgccg ggaacgcgtg
    16741 agggagtagg ttgtcgccgc gcgttacgac tgcggtttga agtagagcac cagccccgcg
    16801 ccgacggaga cgagcaggaa cccgacgtag agcatcgggt tgatggcggt cttgggcggg
    16861 tgaagccaga gcgtgacgag gacgttgacg agcggcgcgc cgccgaagac gagcggcatg
    16921 acgtaggtgg ggaggccgcc ggtacggaag gcgaagatga tgcagaccgc gccgatggcg
    16981 ccgagtgcgc ccgcgagagt cgccgtcgtc gcgccgccgg agttgaagtt gttcaacgcg
    17041 ccctgcgacc agagccaagc ggcgggcacg agcacgccga tcaaaaagta ggccacgccg
    17101 acgcagagca gcgcgcgcat cgggttgccg agtttgacct gcccctgatg cagcatcgcg
    17161 ccgtagactc cccacgacaa agccgcgccg agcgcaaaca cgatccagac catagatttt
    17221 tcttttcctt tttccgacat cattttccag agcttcgcag cgtgtagtta atcataactt
    17281 tgcgcctccg gcgagaaatg atcttcccac cggaagcgca gaactggaaa cgtaaggcgc
    17341 gaagggttga gcctcgacgc gtgaagttta actcaactca gaactgaacg cgcacgccga
    17401 gttgaatttg acgcgcggag gtgacggtcg agcggatgcg cccgaaggtc gggagcggtt
    17461 gttcgttgtc agccgcaccc gcaaagacgg tcaggccggg cggcgcgaag ttggcgcgat
    17521 tgaagagatt gaacgcctcg acgcgaatct gaacattcgt gttttcgccg agcggcgagc
    17581 cgaagcgaat attcttcatg gcggagaggt cgaaggtgcg caggtttgga ccgatgaacg
    17641 cgccgcgccc ggtgttgccg agcgtgcccg ccggttgcag gacgaaagcg ttcgggtcga
    17701 agtattgatc ggggtggccg aggacggcgg actcgtaggt gtagccgggg gcgatgctgg
    17761 cgcggtcgcg cccgatgccg gggccgagcg acggttgcca gagcgagcgc gagcggttgg
    17821 cctgcacgaa gacggtcagg gggttgccgc ttctggcctg cccgattccg gcgagttgcc
    17881 agtcgtcgag aagcgcgccc gcgatgccgt caagattctt cgcaaaggga atctgccaga
    17941 cgaagttgac cacccaattg tgcttggcgt gataatcgga gagtcccttg ttgtagttga
    18001 aaccgggaaa ttcgggaaag gcggtggtcg tgccgttcgt ggcgtcggag aagaaggtcg
    18061 aagcctgcgt cgtgtcgatg ttgcgcgaga aggtgtaaga ggattggacg ttgaagttct
    18121 gattccaacg cttgcgcacc tcgaagatca tggcgttgta ccaagagtta ccgtcgctgc
    18181 tcttgagttc gatggtcgag aacgcggtgt tctggcgcgg cgcgcccgcc gggtagaaag
    18241 gcgtgccgtc gggtcgtatc tgcgcgacgg cggtgttcac gtcgttgctg cgcaagaggt
    18301 gtacgccgcg cgaaccggcg tagccgagcg tcacgaccgt ctgccagggg agttcctgct
    18361 ggacgttcag gttgtagacg ttgagatacg ggttatcgag atcgtactgg acggggcgga
    18421 tcgagttggc gaaagcgcac gagagcggcg cgacggggaa ggccggacgg cagggcgagg
    18481 tcggcgtgaa gccgatggcg atgcgcggcg tggcgggcgg gttcgtcacc gtcacgatca
    18541 ggttctgctg gttgttcgtg ttgaagtaga cgccgtagcc gccgcgcaga gaagtcttgc
    18601 cgttgccaaa cacgtcccac gcgaagccga cgcgcggcga gaggttcttg cgcgtagggt
    18661 tttggtagag cacgccggtc gtgggcgcgc ggtcagtaag agagggcagg gcggaatcgc
    18721 gcccgtagag gtctacgggc atggtcgtcg tttcataacg cagcccggcg ttgagcgtga
    18781 gccgttcgtg gaggcggaag gcgtcctgca cgtatccggc gaagagcgtg aaacgccagt
    18841 agcggtcgag cgcgccgttg gcggggaggc cgatgaaact ctgcgggctg ttcgacagga
    18901 agtttctgag attggagaaa gtccatgtgc cgagcgagaa ggtcgggttg tacatgttgt
    18961 cctgataacg ctcgacgagg ccgccgaact tcagcaggtg gcgaccgcgc gtgtgcactg
    19021 cgccgtgctc gaaactgaag acgttctgcg tgagtcgcag gtcggctgaa ctctggggtc
    19081 cgaagcgcgg cacgccgccg atgtctatgt tgccgatgag cgtgcgaccg ggaataaacg
    19141 gcgtcaggtt ttgcgtcggg ttcgactcga cgttctgccc gatgcgcgtg cgcccgaagc
    19201 cgaagcggag ggtgtggagc gtgcgcgccg actggatgaa gcgatgctcg gcggtgaaaa
    19261 actggttgcg cgagagaaag gcgcggggaa actgcggaaa gtcggtcggg agagcctgat
    19321 ccgcgtcgtc gaaagtgtag cgggcgaaga attggttgcg gtcgttgaag ttgtgatcga
    19381 tgcggccttg aaagaagtgc tgcgtgacct gttgattgaa gccgaaattg taggccgcga
    19441 ggccgccgcc gaggttcgag ccgttcggca gcgggaactc gtcgaggaac gggcgaatcg
    19501 cggggctgat cgtgatctgg ccggggacgc cgggcaggat gccgttgcgc gcgttgaggt
    19561 cgggcacgac ggttgagatc gtgcgcccga gttgttcgcg cagcgattcg tagttgaaga
    19621 agaagaaggt tttgtcgcgc cggatggggc cgccgatgga gccgccgaat tgattgcgtt
    19681 taaactcggg tttacgcggc gtgtcgaaaa agttgcgcgc gtcgaagttg tcgttgcgat
    19741 gaaactcgta gagcgagccg tgatattcgt tcgtgcctga cttcgagacg gcgttgatct
    19801 ggccgccgga gttgcgcccg aactccgcgc tgtaggcgtt cgtctccacg cgaaactcgc
    19861 gtatgccttc gatgccgagc acggtgcccg ccgcgctccc ggcggggccg ttcgtgaaat
    19921 cgttctgcgg cgtgccgtcg atcaggtaga cgttcgagcg cgggtcttgg ccgttgatgc
    19981 tggtagcgag tccgtgtgcg accgccgagc cgccgtcgcg gtgcgggtag gagatgacgc
    20041 cgggttgcag gagcgcgagg tctgtgtagt tgcgcccgtt caagggcaac tcgcggatcg
    20101 cgtcttcgtt gacgagatag ctcaactcgg aagtctgcgt gttgacgagt tcggcggagc
    20161 cggtcacttc aacctgcgtg tctatcgagg ccacttccag agtgatgttg agcacggcgg
    20221 tttcgccgat ggtgagacgc aggcgttcct gcacgaaggg cttgaagcca tcgcgcgccg
    20281 agcgtatttc gtaggttccc acgggcatgc cggggaagac gaaacgccct tcatcttcgc
    20341 tgacagcggt gcgcgtaaat cccgtctcaa ggtttcgcgc cgtgacctcg acgccgacga
    20401 gcacgccgcc ctgcgcgtct ttgatctcgc ccgtgatcgt cgcgtcggtc tgctgcgcga
    20461 aggaagcgga agcgaggcag agaacgaaac ttgcgagcag cagtagacgt aatttgttgc
    20521 tgaagttaat ggttgaaaat ttcatgggaa cggtttagtg ctttctgggt tgtgacgttt
    20581 tgctgtttgc gctccgggga acacaagtat tttaagcgtt gccttgacgt gtgacaatcg
    20641 tgtgcgacac tatacgttct tacggcgaaa gtggcctcgt ggtttcttcc aaaaatctgt
    20701 tccggcctgt acgaataatt gaatgaaacg aatcctcctg accttggtgg tcatcgcgct
    20761 ctacatctcg caccaagaca tctggaactg gcgtaaagtt tatccgatgg ccttcggctt
    20821 cattcccatc ggcctctttt atcacgcctg tttcgtcgtc gccgcgtcgc tcgtgttgtg
    20881 gatgctcgtc acttacgcat ggccggcgca tcttgaagcg gaagtcgagg ggcagcaggg
    20941 gacgaaagcc gcgacggcga aagaggagga cgcgcgttga ttccagccat cgtcgttttt
    21001 gtttatctcg ccgtcgtcgt ctatatcggc atcttcgcgt ttcgcaaagc gacgggcaaa
    21061 gagaaggcgg aagactattt tctcgccaat cgctcgctcg gccccttcgt cttcctgctc
    21121 tccctgttcg ggacgaacat gacggcgttt gccatcctcg gctcatccgg ccacgccttc
    21181 aacaacggca tcgtcacctt cggtttgatg gcgacctctt ccgcgctcgt catcccgctc
    21241 acgatctttc tgtgcggcac gcgcatctgg gctttgggga agaagcacgg ttttatgacg
    21301 cccgtgcaga tgtttcgcga ccgctgggac tgcggccata tcggcacggt gatcttcgtc
    21361 gtgcaggccg cgctccttgt cccctacatc atcatcggcg tgatgggcgg cggcaccacg
    21421 ctcaacgtga tcagcggcgg cctcgtgccg ttctggttcg ggggggcaat cgtcgcgctc
    21481 gtcgtgatgt cttacgtctt cttcggcggg atgcgcggca cggcctgggt caacgcgttt
    21541 cagacgatcc tctttctcgt cttcggcgct atcgccgtga tcgtcatcgg cgcggggatg
    21601 ggcggctttc agtctgccat caacggcctg ctcgcttcgc ccgccaccgc gccgctcctg
    21661 acgcgcgaac gcatctcgcc ttactacttt ttcagctaca ctttcatccc gctctcagcc
    21721 atcgcctttc cgcac
//
LOCUS       DASWNS010000143         5435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_343145, whole genome shotgun sequence.
ACCESSION   DASWNS010000143 DASWNS010000000
VERSION     DASWNS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343145"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VGB76_04855"
     CDS             complement(<1..546)
                     /locus_tag="VGB76_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098266.1"
                     /translation="MQSLPHRSLTTCLVSLILASLCTAQHAPSTKTPAKDGAALKGKT
                     VAAMSPEEKVVRAAYEKLTLLNKAARLSKNQAVKSSVDDVQALVFELSNFRIGPIRDI
                     WGALHSKIKTDGSGDVIRLGRSVRRLNKGPEHVAYEAQWTTGQYASIYDRQWTIGDLL
                     SYEPDKYYDVGEYALYDVTVSF"
     gene            complement(606..1106)
                     /locus_tag="VGB76_04860"
     CDS             complement(606..1106)
                     /locus_tag="VGB76_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098267.1"
                     /translation="MSFDTCSKLATMRRNISGLLVALLTLGVGALIVRENSPSPKTQN
                     FGVRSYSSSDYKNMFWAWETYSSPQEATTKFNAQLQQATQYMEFTPCFDSNGRRIGER
                     AVMHTTSPSSPQPVWRIMWTQQSADFSESFTVESPLLSEARHGETTGQEGWKKCISTK
                     REANPK"
     gene            complement(1103..2116)
                     /locus_tag="VGB76_04865"
     CDS             complement(1103..2116)
                     /locus_tag="VGB76_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0098268.1"
                     /translation="MLLLLIGNCAGGALTPVRAQGDENVVAVVNGRRITHRDVDTSII
                     SKIFPLQQQIHALRKTALENFIISTILEDEAKRKGISVEELRKRLTAGKAEVGADEVE
                     QVYLENAVTFASMSPDEAKERIRLDLETQARMRNYREALLKLKKSSGVQVLLEEPMLP
                     SVAVGDVAPSAGPKDAPVIITEFADFQCVYCKQAQSVLKQAIQSYGNDLRLIFKHLPL
                     DTHPQAFAAARAAFCAGEQGSFWPYHDGLFASENLSPETFTRTATSIGLDLSKFKGCV
                     GSEASRAAVLKDVQEARRLGINGTPAYIINGKLHRGALGFEELKSIIEQELKPTQTIS
                     RKQ"
     gene            2552..3793
                     /locus_tag="VGB76_04870"
     CDS             2552..3793
                     /locus_tag="VGB76_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0098269.1"
                     /translation="MAEFICRLGTPAGEVVTRTIEAVGVAEARVRLESEGFRVFSVTP
                     PKARGIAAMTRGGKGGTTPRIKSGDFLLFNQQLSALLRAGIPILQSVAMLRRRASSSR
                     LRGMLGDVEEKIRGGMALSAAFAAQGAVFPRIYTASILAGERSGALDEVLNRYNAYMR
                     RGVEIRRKIRGALAYPLFLLIASFGMVTFLTTYVVPRMARLFEGFGRNLPAITLVVVG
                     ISNWMAANIWWFAPLVIIGSIAFYLWSRTPHGRLSIDRFVLKLPIAGQLVIQLSVAQV
                     TRSLSTLLAGGITIVESWEIAAEAITNRELRARSTGILPMIREGRSFTESLEAANWLP
                     ELGMDMIGVGERSGSLREMLDEVATFYDAESEVRLEQLTTVLEPAILLVMGGVVVTIL
                     LAIYLPIIQSISNVSGTPGGG"
     gene            3875..>5435
                     /locus_tag="VGB76_04875"
     CDS             3875..>5435
                     /locus_tag="VGB76_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY0098270.1"
                     /translation="MSKTLDDRLTLEEGETTLVRDANELPARAVNGAGANENARGGFA
                     EPHASLPPEVRAAQELARRYRLPYIDLLPPEGDSPIDYALLSEIPVDLLLRYQVVPLR
                     REGRRFHVAMADPSDLERLDELASALRARIVPHVATAGAIDVVLKKGDATQRVLQEAT
                     SNFRISLVKETEHGDEVLDLDRLATDSDMSPIIKLVDTIIFNAMESRASDIHIETRDT
                     EVQVKYRIDGALYAKVDPIDLAYHQTLISRIKVMSELDIAERRVPQDGRFRVRYKGRT
                     VDFRVSIMPTVHGEDAVIRILDKEQINEEFKDLRLDVVGFDVNDLRNFRRYIAEPYGM
                     VLVTGPTGSGKTTTLYAALNEIRNEEDKIITIEDPVEYQLHGITQIPVNEKKGLTFAR
                     GLRSILRHDPDKIMVGEIRDAETAQIAINSALTGHLVFTTVHANNVLDVLGRFLNMGV
                     EAYQFVSALNCVLAQRLVRNICVHCKRPATVTRAQLEESALDPGLEHSGLFFEGAGCM
                     ECGGTGYRGRTA"
BASE COUNT         1085 a         1576 c         1598 g         1176 t
ORIGIN      
        1 aaaagagact gtcacatcgt acagcgcata ctctccgaca tcgtagtact tgtccggctc
       61 gtagctcagc aggtcgccga tagtccactg ccggtcgtag atagaggcat actggccggt
      121 tgtccattgc gcctcatacg ccacgtgctc cggcccttta ttcaaacgtc tgaccgagcg
      181 cccgagacgg atgacatccc ctgagccgtc ggtttttatc ttgctgtgca atgcacccca
      241 gatgtccctg atgggtccga tgcgaaagtt gctcagttca aacacgagcg cctgaacgtc
      301 atccacggag cttttgacgg cctgattttt actgagccgc gcagccttgt tgagcagggt
      361 taatttctca taagccgctc tgacaacttt ctcttcggga ctcatggcgg ctactgtctt
      421 gcctttcaga gcggctccgt ctttggccgg agtcttagtg gaaggagcgt gttgtgcggt
      481 gcataagctg gcgaggatca gactgacaag gcaggtggtg agggatctgt gagggagact
      541 ttgcattttt ctttcccttt cgctcagaca aggctgagtc tggttgtatt ggtggaagag
      601 ctatttcatt ttgggtttgc ctctcgtttt gtggaaatgc acttcttcca gccctcttgc
      661 ccggtcgttt cgccatgtcg agcctctgac agcagcggtg actcaaccgt aaatgactct
      721 gaaaagtctg cgctctgttg ggtccacatg atgcgccaga ctggttgcgg cgaagagggc
      781 gaggttgtgt gcatcactgc ccgctcgcct atgcggcggc cattgctatc gaagcacggg
      841 gtgaattcca tatactgggt tgcctgttgc agttgcgcgt tgaatttggt ggtcgcttcc
      901 tgaggagatg agtaagtctc ccaagcccag aacatgttct tatagtcgga agagctataa
      961 ctgcgaacac caaaattttg agttttcggt gaggggctgt tttctctgac gatcagcgca
     1021 ccgacgccga gcgtcaataa cgcgaccaat aaacctgaga tattacgtcg catagttgct
     1081 aacttgctac aggtgtcgaa gctcattgtt tgcgggagat ggtttgagtg ggtttcagtt
     1141 cttgctcgat gatgctcttg agttcttcaa aaccgagcgc gccgcgatgc agcttaccgt
     1201 tgatgatata ggccggtgtg ccgttgatgc ccagccgccg cgcctcttgc acatctttca
     1261 gcacggcggc gcgagaagct tcggagccga cacagccttt gaatttcgag aggtctaggc
     1321 cgatgctcgt cgccgtacgg gtgaaggttt ccggcgagag attttcggag gcgaacagtc
     1381 cgtcgtggta aggccagaaa gagccttgct ctccggcgca gaatgccgcc cgcgccgccg
     1441 caaacgcttg cggatgagtg tcgagcggga ggtgcttgaa gatcaaacgg aggtcgtttc
     1501 cgtaactctg gatcgcctgc ttgaggacgc tttgagcctg cttgcagtag acgcactgaa
     1561 aatccgcaaa ctccgtgatg attaccgggg catctttagg tccggctgaa ggagcgacat
     1621 ctccgaccgc gacggaaggc agcatcggct cttccaacag gacttgcacg ccggaacttt
     1681 tcttcaactt caacagcgcc tcgcggtaat ttcgcatccg cgcctgtgtc tccaaatcga
     1741 gcctgattct ttctttcgcc tcgtcaggac tcatcgaagc gaaagttacc gcattctcaa
     1801 gataaacctg ctcgacttcg tccgccccca cttcggcttt cccggcggtc aaccgcttcc
     1861 tcagctcttc cacggagatg cccttccgct tggcttcatc ttcgagaatc gtgctgataa
     1921 taaaattctc cagagcggtc ttgcggagag cgtgaatctg ttgttgcagt gggaaaattt
     1981 tagagatgat ggaagtatca acgtcccggt gagtgattct tctgccgttg actactgcga
     2041 ccacattctc gtcgccctgt gcacggactg gcgtgagtgc gcctccggcg cagttgccga
     2101 taaggagcaa gagcaaaagt ttaaggaatg tgggaaggcg tgagacgtag gttgaggtaa
     2161 ggtgagacat gtacatttgt aaagtttact cgcccgaatg aaggtggttg agttgacccc
     2221 cgaatccgcg taggtaaatt ctgaaaacgt gaggcggcct aaaaacttcg agtcagcggt
     2281 cggtagaaaa ttgaattgtg acagctcact gcaagaactg cgtcagatcg ctatatacgc
     2341 ttcattcttg cggctgtcaa gaaaaatttt caacctgtaa acgcctgact ttgcaacttt
     2401 ctgtgcaact tcccacggcg ggcggcgtgt aaagacgggc ggtgaagtcg tgcgcggggg
     2461 cgtgggagtt ggggcgcggc gcgtgttata ctccgttccc cgtttaatgg acgatttcac
     2521 cgaccgtgaa atcattgaat caataaaaac aatggccgag tttatttgtc gtttgggaac
     2581 gcctgcgggc gaggtggtga cgcgcacgat tgaggcggtg ggcgtggccg aggcgcgcgt
     2641 ccggcttgag agcgagggtt ttcgcgtctt ttcggtgacg ccgccgaaag cgcgcggcat
     2701 cgccgccatg acgcgcggcg gcaagggcgg caccacgccc cgcatcaagt ccggcgattt
     2761 tctcctcttc aatcagcaac tctccgcgct gctgcgcgcg ggcatcccga tcctccaatc
     2821 ggtcgccatg ctccggcggc gcgcgtcgag ttcgcgcctg cgcggcatgc tcggcgacgt
     2881 ggaagagaag attcgcggcg gcatggcgct ctcggcggcc ttcgccgcgc agggggcggt
     2941 cttcccgcgc atctacacgg cctccatcct cgcgggcgaa cgctccggcg cgctcgacga
     3001 agtgttgaac cgctacaacg catacatgcg tcgcggcgtc gaaatccgcc gcaagattcg
     3061 cggcgcgctc gcctatccgc tctttctgct gatcgcctcc ttcggcatgg tcacgttttt
     3121 gaccacctac gtcgtgccgc gcatggcgcg actcttcgaa ggcttcgggc gcaatctccc
     3181 ggcgatcacg ctcgtcgtcg taggcatctc gaactggatg gcggccaata tctggtggtt
     3241 tgcgccgctc gtcatcatcg gctcaatcgc cttctatctc tggtcgcgca cgccccacgg
     3301 gcggctctcg attgaccgct tcgtgctgaa acttcccatc gccggtcaac tcgtaatcca
     3361 actctccgtg gcacaggtga cgcgctcgct ctccactctg ctcgcgggcg gcatcacgat
     3421 tgtcgaatca tgggagatcg cagccgaggc catcacgaat cgagagttgc gcgcacgctc
     3481 gacgggcatc ctgcccatga tcagggaagg gcgttcgttt acggagagcc ttgaggcggc
     3541 aaactggctg ccggaattag ggatggatat gatcggcgtg ggcgagcgtt cgggcagttt
     3601 gcgcgagatg ctcgacgagg tggcaacctt ctacgatgcc gagtcggaag tccggcttga
     3661 acagttgacg accgtgctcg aacctgcgat tctgctcgtg atgggcggcg ttgtggtcac
     3721 gatcctgctg gcgatctatc tcccgattat tcaatccatc tcaaatgtct caggaacgcc
     3781 cggcggcggt taataacggg caatcctcca gcccttttct cgcatctgac acaggttgcg
     3841 aagtttagac ggaagagaag ttcagaagac cataatgagt aagaccctcg acgacaggct
     3901 cacgctggaa gaaggcgaga ctacgctcgt gcgcgacgcg aacgaactcc ccgcccgcgc
     3961 tgtaaacggc gcaggcgcaa atgagaacgc gcgcggcgga ttcgctgaac ctcacgcctc
     4021 gctgccgccc gaagtgcgcg cggcgcagga actggcgcgc cgctatcgcc tgccctacat
     4081 cgacctgctg ccgcccgaag gcgattcgcc gattgactac gcgctgctca gcgagattcc
     4141 cgtcgatctg ctgctgcgct atcaagtcgt gccgctcagg cgcgaaggcc gacgcttcca
     4201 cgtcgcgatg gccgatccgt ccgatctcga acgcctcgac gaactggcga gcgcgctccg
     4261 tgcgcgcatc gtcccgcacg tggcgaccgc cggggcgatt gacgtggtgc tcaaaaaggg
     4321 cgacgccacg cagcgcgtcc tgcaagaggc gacttccaac ttccgcatct cgctcgtcaa
     4381 agaaaccgag cacggcgacg aagtcttaga ccttgatcgt ctggcgaccg acagcgacat
     4441 gtcgccgatc atcaagctgg ttgacacgat catcttcaac gcgatggaat cgcgcgcctc
     4501 cgacattcac atcgaaacgc gcgacacgga agtgcaggtc aagtaccgca tcgacggcgc
     4561 gctctacgcg aaagtcgatc cgattgatct cgcctatcac cagactttga tctcgcgtat
     4621 caaggttatg tcggaactcg acatcgccga acggcgcgtg ccgcaggacg gtcgcttccg
     4681 cgtccgctac aaagggcgca cggttgactt ccgcgtctcg atcatgccga ccgtgcacgg
     4741 cgaagatgcc gtcatacgta ttctcgacaa ggaacagatc aacgaagagt tcaaagacct
     4801 gcgcctcgac gtggtcggct tcgacgttaa tgatctgcgc aacttccgcc gctacatcgc
     4861 ggagccttat ggcatggtgc tcgtcacggg accgaccggt tcgggcaaga cgaccacgct
     4921 ctacgcagcc ctgaacgaga tcaggaacga agaagataag atcatcacga ttgaagaccc
     4981 ggtcgagtac cagttgcacg gcatcacgca gattcccgtc aacgaaaaga aggggctaac
     5041 gtttgcgcgc ggcctgcgct cgatcctccg ccacgacccg gacaagatca tggtcgggga
     5101 gatccgcgac gccgaaacgg cgcagatcgc gatcaactcc gccctgacgg gccacctcgt
     5161 attcacgacg gtccacgcga acaacgtcct tgacgtgctg ggccggttcc tgaacatggg
     5221 ggtggaggca taccagttcg tctccgcact gaactgcgta ctggcgcagc ggctggtacg
     5281 gaacatctgc gtgcactgca agcggccggc aacggtcacg cgtgcccagc tcgaggaatc
     5341 cgcactggat cccgggctcg agcatagcgg actcttcttc gagggggcgg gctgcatgga
     5401 atgcggcggc actggatacc ggggccgcac cgcga
//
LOCUS       DASWNS010000144         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_188365, whole genome shotgun sequence.
ACCESSION   DASWNS010000144 DASWNS010000000
VERSION     DASWNS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188365"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VGB76_04880"
     CDS             <1..592
                     /locus_tag="VGB76_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098271.1"
                     /translation="VILGYSIGGAFIGDGSELNERYYDAEGDNYDTDESVVRTPSERA
                     FASQAIIGGAYGSTAYAQPQSTAARSTAGQTDSGDTEPQPRPSYSSGYDMAPDSSSSS
                     SSLAASEPQKPGLFERFKETQAYDKLQAEVATLGSRAVEELSKTAQAVVLPALLGKLK
                     DMVGIDLSTQREVAQRSQLEHQAAAASASTGEETRK"
     gene            726..1724
                     /locus_tag="VGB76_04885"
     CDS             726..1724
                     /locus_tag="VGB76_04885"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0098272.1"
                     /translation="MTLEPVAQLTRNPNAPPETATRRAATSPPTRVEIILNASAGSGD
                     QEAAQRTLAEIFATESNLEAHVALARSGEEIVALAREAAANPEVQIVVAGGGDGTVNA
                     VAAELVGTSKTLGVLPLGTLNHFAKDLRIPLDLEGAARNLIEGEQVKVDVGEVNERIF
                     INNSSLGLYPSIVRHREKQQERLGRGKWFAAIWATLEVFRRYPLFSVRLSADGQQFRR
                     RTPFVFIGNNEYQMDTFNLGARSCLDAGQLSLHLTRDIGRWGLVRLALSALLGRLREA
                     RDFDALCTKEVWIETRRTRVRVATDGEVNIMRSPLHYRVRPAALRVIVPRTVTSDK"
BASE COUNT          386 a          602 c          562 g          293 t
ORIGIN      
        1 cgtcatcctc ggttacagca tcggcggggc gttcatcggc gacggctcgg agttgaacga
       61 acgctactac gatgcggaag gcgacaacta cgacacggac gaaagcgtcg tccgcacccc
      121 gagcgaacgc gccttcgcct cgcaggccat catcggcggc gcatacggct cgaccgctta
      181 tgcccaaccg cagtccaccg ccgctcgctc gacggcaggg cagacggact ccggcgatac
      241 ggaaccgcaa ccgaggccgt cttacagcag cggctacgac atggcacccg acagcagcag
      301 tagcagcagc agtctcgctg ccagcgaacc gcaaaagccc ggcctcttcg aacgcttcaa
      361 ggagacgcag gcttacgaca aattgcaggc ggaagtggcg acgctcggca gccgtgccgt
      421 cgaggaactc tcgaagacgg cgcaggccgt cgtcctgccc gcactgctcg gcaagctgaa
      481 agacatggtc ggcatagacc tctcgacgca gcgcgaagtg gcgcaacgca gccaactcga
      541 acatcaggcg gcggcggcga gcgcgagcac cggagaagag acgcgaaaat gattccggcg
      601 catcaggtgt gaactgtcag gagcagagtg ggcgggcggc gaaagcggct cgcccattct
      661 tttattccgt ggttcgagac aaaaattttc aactcgaaat tcaccctgcc gcccgcgagt
      721 aaaccatgac gcttgaaccg gtagcacaac taacccgcaa cccgaacgcc ccgccggaga
      781 cggcgacgcg gcgtgcggcg acctccccgc ccacgcgcgt cgagatcatc ttgaacgctt
      841 ctgccggaag cggcgatcag gaggctgcgc agcgcacgct cgcggaaatc ttcgcgactg
      901 agagcaatct ggaggcgcac gtcgcgctcg cacgcagcgg cgaagagatc gtcgcgctcg
      961 cgcgagaggc cgccgccaac ccggaagtgc agatcgtggt tgcgggcggc ggcgacggga
     1021 cggtcaacgc ggtcgcagca gaactcgtcg ggacaagcaa gacgctcggc gtgctcccgc
     1081 tcggcacgct caaccacttc gccaaagatt tgcgcatccc gctcgacctc gaaggcgcgg
     1141 cgcgcaatct catcgaaggc gagcaggtca aagtcgatgt gggcgaagtc aacgaacgca
     1201 tcttcatcaa caattcgagt ctcggcctct atccttcaat cgtgcgccac cgcgagaagc
     1261 agcaggagcg tctggggcgc ggcaagtggt tcgcggccat ctgggcgacg ctcgaagtct
     1321 tccgccgcta cccgctgttc agcgtgcgcc tgagcgcgga cgggcagcag tttcgccgcc
     1381 gcacgccctt tgtcttcatc ggcaacaacg aataccagat ggacacgttc aacctcggcg
     1441 cgcgttcctg tctcgacgcg gggcagttga gtttgcacct gacgcgcgac atcggacgtt
     1501 gggggctggt gcgtctcgcc ttgagcgcgc tcctcggacg actgcgtgaa gccagagatt
     1561 tcgacgcgct ctgcaccaaa gaggtctgga tcgagacgcg ccgcacgcgc gtgcgcgtcg
     1621 ccacggacgg cgaagtcaac atcatgcgct cgccgctcca ctaccgcgtc cgacccgccg
     1681 cgctccgggt cattgttcca agaacagtga cgagtgacaa gtgacaagtt aaggcagaag
     1741 aaaaactgtc tttcaacttg tcttgcttcc tgctcgtcac tcgtcactgt tctctcgtcg
     1801 ctggcatgaa actcatcaac gacagcagca ctcttaactt gtt
//
LOCUS       DASWNS010000145        10129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_214155, whole genome shotgun sequence.
ACCESSION   DASWNS010000145 DASWNS010000000
VERSION     DASWNS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214155"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VGB76_04890"
     CDS             complement(<1..371)
                     /locus_tag="VGB76_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098273.1"
                     /translation="MSTIFERPPDDSAATLALITSGGLDASPADEAAVEVAAVRATLG
                     ALPLGAHLILRCRKDWRTATVVRIELEKITLSVSSPTGRTYRMRRPPDAPLSFDNDRH
                     IPLLDAGKPSCWRAGLARYDVR"
     gene            complement(383..1174)
                     /locus_tag="VGB76_04895"
     CDS             complement(383..1174)
                     /locus_tag="VGB76_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEY0098274.1"
                     /translation="MKNMMRMKIAHDARPQARTLLIATLITLALWFLPYTSFLTYPFR
                     LFVTFIHEGGHALAAVLTGSSVQSLSVAIDTSGLTETLSPQGGFFSRFFISSAGYLGT
                     IAFGALLLWLVRRKVKARVVLVGSAAIILGLTVVFGFIAPLANLSLQPFTVIAGVSIA
                     AGLLAAAKYAGLRAANFLVGFLAVQCVLNAVFDLKNVLWMSVASSAPTDAMNMAEATG
                     VPAIVWSLIWIGIAVLILSTAMRAYAVSRNSPSQPDLPFEDPLEV"
     gene            complement(1356..1862)
                     /gene="moaC"
                     /locus_tag="VGB76_04900"
     CDS             complement(1356..1862)
                     /gene="moaC"
                     /locus_tag="VGB76_04900"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY0098275.1"
                     /translation="MNKLSHLDREGRVAMVDTSAKETTVRRAVASARVKMSAETLAAV
                     RAQRTPKGDPLETARLAGIMAAKRTSELIPLCHPLPLTHIEVRAEIEDRGIYIEAEAA
                     TRAETGVEMEALTAASVAALTVYDMCKALDKAMTITDVRLERKTGGKSGDYERRKARG
                     EARKGKPV"
     gene            complement(1859..2695)
                     /locus_tag="VGB76_04905"
     CDS             complement(1859..2695)
                     /locus_tag="VGB76_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0098276.1"
                     /translation="MKNFGGTIAPEAAQTRDGGDSSNKTEKAANEREPVPLAHEAIHE
                     TVARLLGAQTRRGLLLDVPAGEGALAARLLGAGFDVRCCDLYPEIFRLRDVEIKGGDL
                     SGKLPYADETFDFITCVEGLEHIENPQQAIREFARLLRPEGELVVTVPNILNIEERVK
                     WLLHGYTSHFKPISREALARVRAELGELEEIALHINPISYAELRYTLEKYGFEIRKLY
                     RDKPKAHLWAYWPLVAFIRLLTRFTSAEKRRERWSAELQSDEILLGGNTLIVHAVKHS
                     KR"
     gene            2970..3554
                     /locus_tag="VGB76_04910"
     CDS             2970..3554
                     /locus_tag="VGB76_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEY0098277.1"
                     /translation="MKKIFMALMLCMLAVGVSGSNASAQEMMKAEMKNGNPTVGGAAM
                     YKTKNIVENAMESPVHTTLVKAVVAAGLVDTLKGKGPFTVFAPTDDAFNKVGAGTLTF
                     VMQPENKATLTKILTYHVVAGRMDSKAIAKAIKKGKGRATFKTVSGGTLTAMMHGNGV
                     MLTDEKGGTAMVTTADVYQSNGVIHVIDTVVMPN"
     gene            complement(3653..4873)
                     /gene="glp"
                     /locus_tag="VGB76_04915"
     CDS             complement(3653..4873)
                     /gene="glp"
                     /locus_tag="VGB76_04915"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY0098278.1"
                     /translation="MIPVPEAIRIVEAETARLPTEAVALVAARGRVLAEDVIADTDLP
                     PFDRALMDGYALRAADTEGARAGAPVRLRIAGEAAAGAGWRGELGAGEAVRIMTGAPV
                     PAGADSVQQVEVTRETEGGTHVEIERATTPAQFITKRATEITRGEIVLRAGETINAAA
                     MAALASFGYARVEVGTRPRVAVLATGTELVDVAAQPGQDQIRDSNTYSLLAYAEAAGA
                     LAEPLPFAGDDAELLQRVIAASAECADLLVLSGGVSMGVYDFTKTALVALGAEIFFER
                     VSLRPGKPTVFARLGRTLVFGLPGNPVSVSVTFNLFARTAIRAMQGAQQAALTEEQAV
                     LGRAVRGAHERASYLPARLSTDAAGRLVAEPLRWGGSSDFVAFTRADALLIVEAGAAT
                     VEAGTVLRIVRLPA"
     gene            complement(4960..5706)
                     /locus_tag="VGB76_04920"
     CDS             complement(4960..5706)
                     /locus_tag="VGB76_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0098279.1"
                     /translation="MNETRISPVEAAREIFRTRHAGARVLFLAGSVMRGAGTPTSDLD
                     IVVVYEHLPVAYRESFMHGGWPVETFVNDAETLNYFLHESDPQTGFPAMANMVLEGRE
                     IPEPSAFSQSMKRLAAASLAAGPPALSEADMRRLRYTLTDVSDDLRHPRSDAELVAAG
                     ARLYELVADFYLRSRTLWSARGKAIPARLREVDAAFAARLCEAFETLFKSKRPEGVVA
                     LVEEVLEPFGGFLFDGYKLDADNDKRKPLD"
     gene            complement(5868..5944)
                     /locus_tag="VGB76_04925"
     tRNA            complement(5868..5944)
                     /locus_tag="VGB76_04925"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5908..5910),aa:Asp,seq:gtc)
     gene            complement(6005..6080)
                     /locus_tag="VGB76_04930"
     tRNA            complement(6005..6080)
                     /locus_tag="VGB76_04930"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6045..6047),aa:Val,seq:tac)
     gene            6114..6410
                     /locus_tag="VGB76_04935"
     CDS             6114..6410
                     /locus_tag="VGB76_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098280.1"
                     /translation="MISLTTLAHLRGQPRELPFELFLTPASKTDAPVKNSFDVCSPEA
                     GFILKDAFHSVKHPPPCPRFPDVRGVHAAMPGKLHSTALRFRSRADSLIISEMR"
     gene            complement(6447..6650)
                     /locus_tag="VGB76_04940"
     CDS             complement(6447..6650)
                     /locus_tag="VGB76_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098281.1"
                     /translation="MSVIGRLDDQVEAVLINPLKRRERIDDGEAVRDAPTPTPAAPDE
                     ERDKRTPDERGERDAATLPVWML"
     gene            6728..8002
                     /locus_tag="VGB76_04945"
     CDS             6728..8002
                     /locus_tag="VGB76_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0098282.1"
                     /translation="MWKYPSKQKRVWQAALASLLLLALAAGQFNHTFAVQQSSQPGAT
                     QSPPKQRPRRVGSNAPPARETPTPLPPQRSASGGEEVDEDDVVRVDTQLISVPVTVTD
                     RAGRPLTNLRAENFALYEDGQPQRVANFITTDAPFEVALVLDTSGSTRAEVGLIRRAA
                     NAFIDALRPGDRISILAFNSQEDGATKLATVELLTHLTDDRAALQQAIESLGTSNGTP
                     FYDALERVVGEVFREPPRAGLRGRRALVALTDGVDSTSEADFASVRARLEQSGTLAYF
                     IQVNTEDFVEERLLKDCQDDGRLSLSRVQLQRYRKIFDPRAEIADYTNFCQMGPFERM
                     QISRSLYQLARREMAELARDSGGKTFNAADLRDARAAFAQVASDIGKQYSLGYYSTNK
                     ARDGRFRSIKVEVRGLQGAQVSAREGYQAPKS"
     gene            8106..8747
                     /locus_tag="VGB76_04950"
     CDS             8106..8747
                     /locus_tag="VGB76_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098283.1"
                     /translation="MHKLLSQLLTRFAAAALTFAVALCATLSWRALPAPRLPPHTSAG
                     AFAGLTQAAVETPMSPEHAAEAVGRYWIFRRGFAPTEHDIRRPLAIPAPDENLIAFGC
                     VTLTVSLDDARRLRLNGDKAGSLHDPASLNVKLEGIFDEREANRAYKPGMEYRTELPS
                     GERIDRTVIIDAAASSSYGEILELVSLLERTGANPIVLHTGDRRAYAWEAMPE"
     gene            8774..9979
                     /locus_tag="VGB76_04955"
     CDS             8774..9979
                     /locus_tag="VGB76_04955"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEY0098284.1"
                     /translation="MNQSTAFDRIVLIVLDSLGMGEMPDAAAWGDAGADTLGHICASR
                     EVRLPNLQAHGLGNVRPLAHVPPAENPRGAYGRCALRSNGKDTTTGHWEMAGIILEEA
                     FPTYPQGFPTDVIARFVAETGVPGVLGNYPASGTEIIKELGAEHVRTGKPIVYTSADS
                     VFQIAAHEEVIPVERLYEICEAARNILRGEHEVGRVIARPFTGETGAFTRTENRHDYA
                     VPPPRENLLVALSDAGLDVVCIGKIASIYDSLGATVDLKAKNNDQSIDQTIRALGDDT
                     RGLIFTNLVDFDMLYGHRRDVEGYARALEHFDARLPEIEAAMRERDLFILTADHGNDP
                     TYRGTDHTREYVPLIAYGQRARSGVNLGTRRSLADMGQTIAENFGLSLKAGESFLPEI
                     TQVTRRSDE"
BASE COUNT         2000 a         3266 c         3063 g         1800 t
ORIGIN      
        1 cgcacgtcgt agcgcgcgag tccggcgcgc cagcaggagg gcttgcccgc gtcgagcaga
       61 gggatgtgtc tgtcgttgtc gaaagagagc ggggcatcgg gcgggcggcg catgcggtag
      121 gtgcgccctg tgggggagga gacggagagc gtgatctttt cgagttcgat gcggacgacg
      181 gtggccgtgc gccagtcttt gcgacagcgg aggatgaggt gcgcgccgag cggcaatgcg
      241 ccgagcgtcg cccgcacggc ggcgacttcg acagcggctt cgtctgccgg agatgcatcg
      301 aggccgccgg aggtgatcag cgcgagcgtt gcggctgaat cgtccggcgg cctctcgaaa
      361 atggtgctca caggtttgtc gtctaaactt cgagcgggtc ttcaaacggg aggtcgggct
      421 ggctcggact gttgcggctg acggcgtaag cgcgcatcgc ggtcgagaga atcaggacgg
      481 cgatgccgat ccagatgagc gaccagacaa tcgccggaac gcccgtcgcc tccgccatgt
      541 tcatcgcatc cgtcggcgcg ctcgacgcga ccgacatcca cagcacgttc ttcaaatcga
      601 agaccgcgtt caggacgcac tgcacggcga gaaacccgac gaggaagttg gccgcgcgaa
      661 gccccgcgta tttcgccgcc gccagcagtc cggctgcgat ggagacgcct gcgatcaccg
      721 tgaacggttg gagcgaaagg ttagcgagcg gcgcgatgaa gccgaaaacg acggtcaggc
      781 cgagaatgat ggcggctgaa ccgaccagca cgacgcgagc cttaaccttg cggcgcacga
      841 gccagagcag gagcgcgcca aaggcgattg tgccaagata acccgcgctg gagatgaaga
      901 aacgcgaaaa gaaaccgccc tgcggcgaca gcgtctcagt cagcccgctc gtgtcgatgg
      961 cgacgctcaa actttgcacg gagcttccgg tcaggacggc ggccagcgca tgcccgcctt
     1021 catgtatgaa ggtcacgaag aggcggaaag gataagtgag aaaactcgtg tagggaagga
     1081 accagagcgc cagcgtgatt aacgtggcga tgagcagcgt ccgcgcctgc gggcgcgcat
     1141 cgtgtgcgat cttcattctc atcatgttct tcatctcaaa atctccgctc cgctttcggc
     1201 caaaaacttt ctcgcctccg gtgaaactca ttctacgacg aagggcgcga aaaggtaaaa
     1261 gtaaaaaggc gacaggcgac agttaaaccc gaatccccgg ctcccgtagg cactaactat
     1321 aaacgccctt tgccctcccg atgttcctgc cgtttttaga cgggctttcc ctttcgcgct
     1381 tcccccctcg cctttcgcct ctcgtaatcg cccgacttgc cgcccgtctt tcgctccagc
     1441 ctgacatccg tgatcgtcat cgccttgtcg agcgccttgc acatgtcata gacggttaga
     1501 gcggcgaccg aggcggcggt caaagcttcc atttccacgc cggtttcggc gcgcgtcgcg
     1561 gcctcggctt cgatatagat tcccctgtcc tctatttccg cacgcacttc gatgtgcgtg
     1621 agcggcagcg ggtggcacag gggaatgagt tcggaggtgc gcttggcggc catgatcccg
     1681 gcgagccgcg ctgtctctaa cgggtcgccc ttcggcgtgc gctgcgcacg caccgccgcg
     1741 agcgtctccg cagacatctt cacgcgcgcc gaagcgacgg cgcgccgcac ggtcgtttcc
     1801 ttcgcgctcg tgtccaccat cgccacgcgc ccctctcggt ctaggtggga aagtttattc
     1861 atcgcttcga gtgtttgacg gcatggacga tcagagtgtt cccgccgagc agaatctcat
     1921 cggattgcaa ctcggcagac caacgctcgc gtcgcttctc tgcggaggtg aagcgcgtga
     1981 gcaggcgtat gaaggcgacg agcggccagt aagcccacag gtgcgccttc ggtttgtcgc
     2041 ggtagagctt ccgaatttcg aagccatact tttcgagcgt gtagcgaagc tccgcataag
     2101 agatcgggtt gatgtgcagg gcgatctctt ccagttctcc caactccgcg cgcacgcgtg
     2161 cgagggcttc gcgcgagatg ggtttgaagt gcgaagtgta gccgtgcagc aaccacttca
     2221 cgcgctcctc tatgttgagg atgttgggga ctgtgacgac aagctctccc tccgggcgca
     2281 gcaggcgagc gaattcccgt atggcctgtt gcgggttctc gatgtgctcc aagccttcga
     2341 cgcaggtgat gaagtcgaaa gtctcgtcgg cgtaagggag cttgccggag agatcgccgc
     2401 ctttgatctc gacatcgcgg aggcgaaaaa tctccgggta gagatcgcag cagcgcacgt
     2461 cgaagcccgc gccgagcagt cgggcggcga gcgcgccttc gcccgccgga acgtcgagca
     2521 ggaggccgcg tcgggtctgc gcaccgagca ggcgcgccac cgtctcgtga atcgcttcgt
     2581 gcgcgagcgg cacgggttcg cgttcgttcg ccgccttctc cgtcttattg ctgctgtcac
     2641 cgccgtcgcg cgtctgtgca gcttcgggcg cgattgttcc gccgaaattt ttcatgcaaa
     2701 gaaactaaac atcaaaacgt gacggatacg caagagactg cgcacctctt cgccggaaat
     2761 cataaaaccg atccctcgcc cgcccccgta aatccggcag acgcacagaa cgagagagac
     2821 agtttttgtg cgacgaactt tttttcgttc gatgaaacac cgaagtgacg gcgactgccg
     2881 aagctctcta agcgtgccgg tcgccgtgcg gacgaggtgt gtcattcgag gagccattcc
     2941 aacagtttga agcttgaaag ggacaaaaaa tgaagaagat ttttatggcg ttgatgctct
     3001 gtatgctggc cgtgggcgtg agcggttcga acgcctctgc acaggaaatg atgaaggccg
     3061 agatgaagaa cggcaatccg accgtcggcg gcgcggcgat gtacaagaca aagaacatcg
     3121 tggagaacgc gatggagtcg ccggttcaca cgacgctcgt gaaggcggtc gtggccgccg
     3181 gtctggttga cacgctcaaa ggcaaagggc ctttcaccgt gtttgcgccg accgatgacg
     3241 cgttcaacaa agtgggcgca ggcacgctca ccttcgtgat gcagcctgag aacaaggcga
     3301 cgctcacgaa gattctgacc taccacgtcg tcgccggacg gatggactcc aaagccatcg
     3361 cgaaggcgat caagaagggc aaaggccgcg ccaccttcaa gaccgtcagc ggcggcacgc
     3421 tgaccgcgat gatgcatggc aacggcgtta tgctgaccga cgaaaaaggc ggcacggcga
     3481 tggtgacgac tgccgacgtg tatcaatcga acggcgtcat ccacgtcatc gacacggtcg
     3541 tgatgcctaa ctaactttcg catcaccctc agcactcctt agcaaaacga caaaggcgtc
     3601 ttcgcgttca aagcgaggac gccttttgtc gtgtctgaag ttttgcgcct gactaggcgg
     3661 gcaggcgcac gatgcgcagg actgtgcctg cttcaaccgt cgccgctccg gcttcgacta
     3721 tcaggagggc atcggcgcgt gtgaaggcca cgaagtcgga cgagccgccc cagcgcagag
     3781 gctctgccac gagtcgcccc gccgcgtctg tagagaggcg cgcgggcagg taactcgcgc
     3841 gctcgtgtgc gccccgcacg gcgcgtccga gcacggcctg ctcttccgtc aaggccgctt
     3901 gctgcgcgcc ctgcatcgcg cggatcgctg tgcgcgcaaa gagattgaaa gtgaccgaga
     3961 cggagacggg attgccgggc agcccgaaga cgagcgtgcg accgagacgc gcaaagaccg
     4021 tcggcttgcc cggacgcaaa ctcacgcgct cgaaaaatat ttccgcgccc agcgcgacga
     4081 gagccgtctt cgtgaaatca tagacgccca tcgagacgcc gccggagagc acgagcagat
     4141 cggcgcactc cgcgctcgcg gctatcacgc gctgcaaaag ctctgcgtcg tcgcctgcaa
     4201 acggcagggg ttcggcgagc gcgcccgccg cttccgcata agcgagcagc gagtaggtgt
     4261 tggagtcgcg tatctggtct tgaccgggct gcgccgctac gtcaaccaac tccgtgcccg
     4321 tcgccagcac tgcgacgcgt gggcgcgtgc cgacctccac ccgcgcgtaa ccgaaagagg
     4381 cgagcgcggc catcgccgcc gcattgatcg tctcgcccgc acgcaggacg atttcgccac
     4441 gcgtgatctc cgtcgcgcgt ttcgtgatga actgcgcggg cgtcgtcgca cgctcgatct
     4501 cgacgtgcgt gccaccttcc gtctcgcgcg tcacttcgac ttgctgcacg ctgtccgcgc
     4561 ctgcgggcac aggtgcgccc gtcatgatcc gcacggcctc gcccgcgccc aactctccgc
     4621 gccaacccgc gcccgccgcc gcctccccgg cgatgcgcag acgcacgggc gcgcccgctc
     4681 gcgctccttc cgtgtccgcc gcgcgcaacg cgtagccgtc catgagcgcg cggtcaaacg
     4741 gcggcaggtc tgtgtctgcg ataacgtctt cggccagcac gcgcccgcgc gccgcgacga
     4801 gcgcgacggc ctccgtgggc aagcgcgccg tctcggcttc gacgatgcga atggcttcgg
     4861 gaactggaat cataagagcg acgctggcaa aaattttgcc acgcctgaga gagacgcggg
     4921 caaaagtttt caccccgttg gttcaatcga ggagccgggt caatcgagcg gcttgcgctt
     4981 gtcgttgtcc gcgtcgagtt tataaccgtc gaagagaaaa ccgccaaacg gttcgagcac
     5041 ttcttcgacg agcgcgacaa ccccttcggg acgcttgctc ttgaagagcg tctcgaacgc
     5101 ctcgcacaaa cgcgcggcga acgctgcatc cacttcgcgc aggcgagccg gaatggcttt
     5161 gcccctggcc gaccagagcg tgcgcgagcg caggtagaaa tcggcgacaa gttcgtagag
     5221 acgcgcgccc gccgcgacga gttcggcgtc gctgcgcgga tggcgtaggt cgtcagacac
     5281 gtcggtcagc gtgtagcgca ggcgtcgcat gtccgcttcg ctcagggcgg gtggtcctgc
     5341 ggcgagcgag gcggcggcca gtcgcttcat agattgcgag aacgcgctcg gttcgggtat
     5401 ctcgcgcccc tcaagcacca tgttcgccat ggccggaaag ccggtctgcg gatcggattc
     5461 atgcaaaaag tagttcagcg tctccgcatc gttgacgaag gtttcgaccg gccagcctcc
     5521 gtgcatgaac gattcgcgat aagcgacggg caggtgttca tagacgacga cgatgtcgag
     5581 atcggaggtg ggcgtgcccg cgccgcgcat caccgaaccg gcgagaaaga gcacgcgcgc
     5641 tcccgcatgt cgcgtgcgga agatttcccg tgcggcctcg acgggcgaaa tgcgcgtttc
     5701 attcatggcc tcaatcgctc tccatccaga tcggaagccg tctgagccat cgggggtatc
     5761 tcccgccagc caggtgcaaa aacaggcaca cccaccgaag tagaccccga aaacacgaat
     5821 ggcctccccg aaggaaggcc aaaagtgttg cgaacaccac atttaagtgg cggagccgac
     5881 gggactcgaa cccgcgacct ccgacgtgac aggccggcgt tctaaccaac tgaactacga
     5941 ctccgcaaaa acttcaaaac tcaaactgcg gcgggcggtg acgctttcgg caactcatcg
     6001 ctaatggtag gcacggtggg actcgaaccc acaacccccg ccttgtaagg gcggtcgtct
     6061 accattgaca gtacgtgcct ctagtgaaga tgcgcgaacc ccggcatctc actatgatct
     6121 cgctcacgac gctcgcccac ctgagaggcc agccccgcga acttccgttc gaactcttcc
     6181 tcacgcccgc ttcaaagacc gatgctccgg ttaaaaacag tttcgacgtt tgttctcccg
     6241 aagcgggatt catcctaaaa gatgcttttc acagtgtcaa gcacccgccg ccatgccccc
     6301 ggtttccaga cgtgcgcggc gtccacgcgg cgatgcccgg caaactccac tcgaccgcgc
     6361 tccggttccg ctcacgcgcc gactcgctca tcatttcgga aatgcggtag aaaataaaat
     6421 agagttcgcg cacacggcct ccaaccttag agcatccaga cgggcagcgt ggcggcgtcg
     6481 cgttcgcctc tctcgtccgg cgtccgctta tcgcgttctt cgtccggtgc ggctggcgtg
     6541 ggcgtcggcg cgtctcgcac ggcttcgccg tcgtcaatgc gctcgcgccg tttgagcggg
     6601 ttgatgagca cggcttcgac ctgatcgtcg agtcttccga taacggacat cttgcggctc
     6661 ctttcagtca tcaagggttt gcagctatat tatggcgcac cgattcgccc gaatgaagtt
     6721 taagtctatg tggaagtatc cctcaaagca aaaacgcgtg tggcaggccg cgctggcttc
     6781 tctgcttttg ctcgcgctcg ccgccggaca attcaatcac accttcgccg tgcagcaatc
     6841 gtcgcagccc ggagcgacgc aaagcccgcc gaaacagcga ccgcgccgcg tcggttcaaa
     6901 cgcgccgccc gccagagaga cgccgacgcc actgccgccg caacgttccg cttccggcgg
     6961 agaagaagtt gacgaagacg acgtggtgcg cgtcgatacg caactcatct ccgtgcccgt
     7021 gacggtgacg gatcgcgccg ggcgtccgct cacgaatctg cgcgccgaaa atttcgcgct
     7081 ctacgaagac ggccaaccac aacgcgtcgc caacttcatc acgaccgacg cgccctttga
     7141 agtcgcgctc gtgctcgaca cttcgggttc cacgcgcgcc gaggtcggct tgatacgccg
     7201 cgccgccaac gccttcatcg acgcgctgcg tcccggcgac cgcatcagta ttctcgcctt
     7261 caactcgcag gaggacggcg cgaccaaact tgccaccgtc gaactgctga cgcatttgac
     7321 cgacgaccgc gccgcgctgc aacaggccat cgagagcctc ggcacgagca acggcacgcc
     7381 cttctacgat gcgctcgaac gcgtcgtcgg ggaagtcttt cgcgagccgc cgcgcgccgg
     7441 tttgcgcggg cgtcgcgcgc tcgtcgcctt aaccgacgga gttgattcga cgagcgaagc
     7501 cgacttcgcc tcggtgcgcg cgcgcctcga acagtcaggc acgctcgcct acttcataca
     7561 ggtcaacacg gaggatttcg tcgaggaacg cctgttaaaa gattgtcagg acgacgggcg
     7621 tttgagcctc tcgcgcgtgc aactgcaacg ctatcgcaaa atcttcgacc cacgcgccga
     7681 gatcgccgac tacacgaact tctgccaaat gggacccttc gagcgcatgc agatcagccg
     7741 ttcgctctac caactcgcgc gccgcgagat ggccgaactc gcacgcgact ccggcggcaa
     7801 gaccttcaac gccgcagacc tgcgcgacgc gcgcgccgcc ttcgcacagg tcgcctccga
     7861 catcggcaag cagtacagcc tcggctacta ctcgactaac aaagcgcgtg acggacgctt
     7921 ccgctccatc aaagtcgaag tgcgcggcct gcaaggcgcg caggtttccg cacgcgaagg
     7981 ctatcaggct ccgaagagtt agaggcagag ttagaccgcc gtgcggaact tcccttaagc
     8041 tcgcggtgtc tgatgtggcg tagacatgtg cccggctgca ttcttcgcca ccgagattgg
     8101 acgacatgca taagctgctt tcgcaactgt taactcgatt tgccgccgcc gcgctgacct
     8161 tcgccgtcgc gttatgcgcc actctctcct ggcgtgcgct tcccgcgccg cgtctgccgc
     8221 cgcacacttc cgccggagcc ttcgccggat taacgcaggc cgccgtcgag actccgatgt
     8281 cgccggaaca cgccgcagag gcggtaggtc gttactggat tttccggcgc ggtttcgccc
     8341 cgaccgaaca tgacattaga cgccccctcg ccatccccgc gccggacgaa aacctgatcg
     8401 ctttcggttg cgtcacgctc accgtctcgt tggatgatgc acggcggttg agactgaatg
     8461 gtgacaaggc cgggtcgctc catgatccgg cgtcactgaa tgtaaagctg gagggcatct
     8521 ttgacgagcg ggaggcgaac cgcgcttaca agcccggcat ggagtatcgc acggagcttc
     8581 cgtcgggcga acgcattgac cggacggtca tcattgacgc cgccgcctct tcaagctacg
     8641 gcgagatact cgaactggta agtctccttg agcggacagg ggcgaatccc atcgtcctgc
     8701 atacaggcga ccgcagggca tatgcttggg aagcaatgcc ggagtagatt ccactgttaa
     8761 taaattacat cttatgaatc aatcaacagc ttttgatcgc atcgtgctca tcgtgctcga
     8821 cagtctcggc atgggcgaga tgccggacgc ggcggcgtgg ggcgatgcgg gggcggacac
     8881 gctcggccac atctgcgctt cgcgcgaagt gcggctgccg aacttacagg cgcacggact
     8941 cggcaacgtg cgcccgctcg cacacgtccc gcccgctgag aatccgcgcg gcgcgtacgg
     9001 tcgctgcgcc ctcaggtcga acggcaagga cacgacgacg ggacattggg agatggccgg
     9061 gatcattctg gaagaggctt tccccaccta cccgcaaggc ttccccacgg atgtcatcgc
     9121 gcgcttcgtc gcggagacgg gcgtgcccgg cgtgctcggc aactacccgg cttcgggcac
     9181 tgagatcatc aaagaactcg gcgcggagca cgtccgcacg ggcaagccca tcgtctacac
     9241 ctccgccgat tccgtctttc agatcgccgc gcacgaagag gtgatcccgg tcgagcgtct
     9301 ctatgaaatc tgcgaggcgg cgcgaaacat actgcgcggc gaacacgaag tcgggcgcgt
     9361 catcgcgcgt cctttcacgg gcgagacggg cgccttcacg cgcaccgaga accggcacga
     9421 ctacgccgtc ccgccgccgc gcgaaaacct cctcgtcgcc ctcagcgacg ccggactcga
     9481 cgtggtctgc atcggcaaga tcgcctccat ctacgattcc ctcggcgcga ccgttgactt
     9541 gaaagccaag aacaacgatc aatccatcga ccagaccatc cgcgcgctcg gcgacgacac
     9601 gcgcggcctc atcttcacca atctcgtgga cttcgacatg ctctacggcc accgccgcga
     9661 cgtggaaggc tacgcacgcg ccctcgaaca tttcgacgcg cgcctcccag agatcgaagc
     9721 cgccatgcgc gagcgcgatc tcttcatcct caccgccgac cacggcaacg accccaccta
     9781 tcgcggcaca gaccacacgc gcgaatacgt ccccctcatc gcctacggcc agcgcgcacg
     9841 cagcggcgtc aacctcggca cgcgccgctc actcgcggac atgggacaaa ccatcgccga
     9901 aaacttcggt ctgagcttaa aggcgggcga aagtttcctg ccggaaataa ctcaagtgac
     9961 gagaagaagt gatgagtgat gagtgatgag taaacctcag tcttgagttg atcaggccga
    10021 gagcaagcca gactgacaca gattgtaatg cagcatcttg agtttgatag tgttccataa
    10081 gccatgccac gaacgactaa agatgcggtc tataaaccga ttccacagt
//
LOCUS       DASWNS010000146         9866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_343190, whole genome shotgun sequence.
ACCESSION   DASWNS010000146 DASWNS010000000
VERSION     DASWNS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9866
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343190"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6059)
                     /locus_tag="VGB76_04960"
     CDS             complement(<1..6059)
                     /locus_tag="VGB76_04960"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEY0098285.1"
                     /translation="MKIKHAPTRLLALTIIFTLLAPPPAGVAATRGGQETVEAATQNG
                     LRFRLSEGREQVERPAPETLAPAELLSAAETKKLLDRLPNLTPEANDTQDFKLRERSL
                     PPPRAGETIQAAFAPPAPNAPPVPTHTRAPLEVLRFAPEGEVALAPAFSITFSQPMVA
                     ASSQEETAASVPVRMKPQPEGVWRWLGTQTLIFQPKVEGGRLPMATNYTVTVPAGTKS
                     ALGNALAVAETFSFATPPPTLKNSHPGGPSEPRDSLMFLEFDQNIDAARVLERLRLQP
                     VATGARLRLATQEEIAADPSVSYFSKQAQAGRWLAVRAVSADGATKNALPPDTNIRIV
                     VPPGTPSAEGARTTLKEQSFAFKTYGALRVTEAFCGYRQRCSPFDQLILTFNNQLDAA
                     KFSPAQVTIKPGMPDAKISVRHNSLFIEGAKRSNTVYTVTLDRAVSDTFRQTLTGTNV
                     FTFKITTATPRLFATGESFAVLDPAGRRGFTVYSVNYPKLKVMLYKVTPADWPQFRRY
                     QAGSARRNGAHTPPGRLVFDEVLEVKAKADELTETSIDLTPALTNGYGQVFVRVEPVV
                     ASSAGRKPRANAYAGRQNSAEAWVQATGIGLDVFADKRELVAWANSLTDGKPLAGVEL
                     TVSPDGLTGTTGADGLARLPFKATFDPKAQQSTLLVARRGDDIAILPQRYSSYNYDDR
                     SSSWRRYDSSETLRWYIFDDRKLYRPGEEVNVKGWIRKVDLTATGDTEMLQGAAGELA
                     HYVLRDAQGNEITKGSVKLNALAGFNLKLQLPATMNLGEAHLAFTFADGSGAHIHQFQ
                     VQEFRRPEFELTARASEAPHFVGTHATVTTAAAYFAGGSLPDTEVNWTVSSKPTSYTP
                     PGRDDYTFGTFYPWWRSDRMSGDRNTKHLQGRTDAEGKHTLRIDFEGVQPARPSSVTA
                     QARVQDVNRQTFASTATLLVHPADVYVGLRSARTFVQKGEAFDISAIVTDLDGRALPN
                     RDVNLRLVRLEYVYEAGSWQPREQEAQEQKIKSGAEAVRVRFPAKEGGMYRLTARVRD
                     ERERLNESELSLWVAGGKRPPLREVAQERVELIPDRKSYAGGETAEILLQSPFAPAEG
                     VLTLRRSGLLRTERFTMNESSYTLRIPLEDGMTPNVNVQVDLVGAATRVNDAGVELAN
                     VPKRPAYASGEINLDIPPASRRLSVKATPREAVLEPGAETLVEVEVKDAAGRAVAGTD
                     TAVVVVDESVLALTAYSLADPLSIFYAHRDEDVNDYHLREKLKLADPEEVKAQMEETA
                     GTSVGTAGGGGGGREVARRSPRIVSNKMEDLPSGARDAMYIIATGSETVEVTANAPPP
                     PQATLAPVGEPAGVIQLRQNFNALAVFAASLPTDANGRAQVKVKLPDNLTRYRIMAVS
                     AAGGRQFGAGEAAITARQPLMARPSAPRFLNFGDRAELPVVLQNQTDAAMSVAVAVRS
                     TNADLTEGAGRRVQVPANNRVEVRFPIAAVKPGMARFQIAAAAGNKADAALVAFPVYT
                     PATTEAFATYGVIDQGAIAQPVKAPSGVVQTFGGLEVTTASTQLQELTDAVIYLVNYP
                     YECSEQIASRVLALAALKDVLTAFKAKDLPTPQAMRDSISVDLKRLQGLQNEDGGFDF
                     WRRGHASVPYVSVHVAHALVRAQSKDFAVPAEMLTRSQAYLRGIESKIPRDYSIEAKR
                     AIRAYALYVRALMKDRDGARARRLIAEAGGVEKLSIESLGWLLPVLSGDAASVQETQA
                     ILRHLNNRVTETAGAAHFADAYSDGEYTILHSDRRADGVILEALIGQQPQSDLIPKLV
                     RGLLSQRKRGRWSNTQENVFILLALDRYFNTYERATPDFVARVWLGGGYAGEQTFKGR
                     SVDRQQLNLPMSALVERTNNEPVNLTIGKEGAGRLYFRIGTQYAPANLTLAAADYGFR
                     VERKYEAIDDPADVRRDADGTWRIRAGARVRVRLSMSNPARRYHVALVDPLPAGLEAL
                     NAALATTEQLPPESTESAVYRGGRGFSDFYWYGRGTWYEHQNLRDERVEAFTSLLWEG
                     AHE"
     gene            6911..7120
                     /locus_tag="VGB76_04965"
     CDS             6911..7120
                     /locus_tag="VGB76_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098286.1"
                     /translation="MEAHSTERITVRDFRQLLESGRHPKLNCPACGQSWLIVGVRRHE
                     THACKACGHRFHLTASLREGAGEGK"
     gene            7117..8199
                     /locus_tag="VGB76_04970"
     CDS             7117..8199
                     /locus_tag="VGB76_04970"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0098287.1"
                     /translation="MRLKPLGQQVVVVFGASSGIGREAALRFAGHGAKVVVAARGEKG
                     LASLVGEIRQSGGEAVAVQAEASDFEQVKRVADVAVERYGRLDTWVHSAAVALYAPFE
                     QTTPAEWRRVVDVNLNGQAYGAWAALPHLRREGRGALIHISSVEALRALPLHSAYAAS
                     KHGIKAFTEALRVELQHDRAQVSVTNIMPASINTPLFNKARTKIGYKPMGLPPIYTPA
                     IVAKAILHAAQNPVREMICGGSGWMIKTGEQLAPHLMDKVLRHAGFEWQRTNEPKSEN
                     EPDNLFHPVEGDDRVSGDFTYQTMPKSIWTWWERHPSAGNLIAGAALGALALTAARRF
                     GSADGGSRARSSADSRAAAASSTAGG"
     gene            8403..>9866
                     /locus_tag="VGB76_04975"
     CDS             8403..>9866
                     /locus_tag="VGB76_04975"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEY0098288.1"
                     /translation="MPGKLYVIGGKVDREGDRTILRKFVRDAGGAEARIVVMTVATDE
                     PQESGDDYRRAFQGLGVSDIRVVDVSHRKDAFEPESLRTIERATAVFFTGGDQLHVTS
                     LIGGTPMQEVLYRRHREGFVLGGTSAGATMMSNTMIIRGDSNRNPRFGAIDIAPGMDF
                     VPNTIIDTHFSQRGRLGRLLTAVAHYPQDLGLGIDENTALVVSGLQFEVIGENSVTVA
                     DGGGMTYTTLPDIEPNESLSLHGVKIHVLAAGQGFDLSERMPIGELPEQSPEKSRSTT
                     ASAVGKSAARMKIESVRTLAGANVYSHRPVLVMRLDLGELNEKESYEIPGFIDRLLAG
                     LPGLRTHHCSKGRPGGFVERLHEGTYFGHTVEHVALELASLAGCSATHGKTRYAGERG
                     IYNVVIEYCAEQATRYLLETAVEFVEALIAGEEFPVQERIEEAKRTMADTELGPSTRA
                     IVEAAERRGIPWIRENEASLIQLGHGKNLRLIQAAMTD"
BASE COUNT         1730 a         2993 c         3301 g         1842 t
ORIGIN      
        1 tactcatgcg ccccctccca caacagcgac gtgaaggctt cgacgcgctc gtcgcgtaag
       61 ttctgatgct cgtaccaggt gccgcgcccg taccagtaga aatcggagaa accacgcccg
      121 ccgcgataga cggctgactc ggttgattcc ggcggcagtt gctccgtggt ggcgagcgca
      181 gcgttcaacg cttcgagtcc ggcggggagc gggtcaacca gtgcgacgtg ataacgccgc
      241 gccggattcg acatcgaaag ccgcacacgc acgcgcgctc ccgccctgat gcgccaggtg
      301 ccgtcggcat cacgccgcac gtctgccgga tcatctatcg cctcgtattt gcgttcgacg
      361 cggaagccgt aatcggcggc ggcgagcgtc aggttcgcgg gcgcgtactg cgtcccgatg
      421 cggaagtaga gccgccccgc gccttctttg ccgatggtca ggttgacggg ctcattgttc
      481 gtgcgttcga cgagagcgga catcggcagg ttgagttgtt gtcgatcaac ggagcgtccc
      541 ttaaacgtct gctcgcccgc gtaaccgccg ccgagccaca cgcgcgcgac gaaatcgggc
      601 gtggcgcgct cgtaggtgtt gaagtaacgg tcgagcgcga gcaggatgaa gacgttctcc
      661 tgcgtgtttg accagcggcc acgcttgcgc tgcgagagca ggccgcgcac gagtttcggg
      721 atgaggtcgc tctgcggctg ttggccgatg agggcttcga ggatcacgcc gtcggcgcgg
      781 cggtcggagt ggagaatggt gtactcgccg tcgctgtagg cgtctgcgaa atgcgccgcg
      841 cccgccgtct ccgtgacgcg gttgttgagg tgacgaagga tggcctgcgt ctcctgcaca
      901 gaagcggcat cgccggacaa gaccgggaga agccagccga gcgattcgat ggaaagtttt
      961 tcgacgccgc ccgcctccgc gatgagcctg cgcgcacgcg cgccatcgcg gtctttcatc
     1021 agcgcgcgca cgtagagtgc gtaagcccga atcgcgcgct tcgcttcgat gctgtagtcg
     1081 cgtggaatct tgctctcgat gccgcgcagg taggcttgcg acctggtcaa catctctgcg
     1141 ggcacggcga agtccttgct ctgcgcacgc accagagcgt gcgcgacgtg gacggagaca
     1201 taggggactg aagcgtggcc gcgccgccag aagtcgaagc caccgtcttc gttttgcagc
     1261 ccttgcaggc gtttgagatc gacgcttatc gagtcgcgca tggcctgcgg cgtggggagg
     1321 tctttcgcct tgaaggctgt gagcacgtct ttgagcgcgg cgagggcgag gacgcgcgaa
     1381 gcgatctgct cggagcactc gtatggatag ttgacgagat agatgacggc gtccgtgagt
     1441 tcctgcaact gggtcgaggc ggtcgtcact tcgaggccgc cgaaagtctg caccaccccg
     1501 gacggcgctt tgacgggttg tgcaatcgcc ccttgatcga tcacgccgta ggtggcgaag
     1561 gcttcggtcg tggcgggcgt atagacgggg aaggcgacga gcgcggcgtc ggctttgttg
     1621 cctgcggcgg cggcgatctg aaagcgcgcc ataccgggtt tgaccgccgc gatggggaag
     1681 cgaacttcca cacggttgtt ggcgggaacc tgtacgcggc gacccgctcc ttccgtcagg
     1741 tcggcgttgg tcgagcgcac cgcgacggcg acgctcatcg ccgcgtcggt ctgattttgc
     1801 aggacgaccg gaagttccgc gcggtcgccg aagttgagga agcgcggagc ggaggggcgc
     1861 gccatgagcg gctggcgggc ggtaatggcc gcttcgcccg cgccgaattg tctgccgccc
     1921 gccgccgaga cggccatgat gcgatagcgc gtcaggttat cgggcagctt gaccttcact
     1981 tgagcgcgac cgttcgcatc ggtcgggagc gacgcggcga agacggcgag ggcgttgaag
     2041 ttctggcgaa gctggatcac tcccgccggt tcgccgacgg gcgcgagcgt ggcctgcggc
     2101 gggggcggcg cattggcagt cacttctacc gtctccgacc ccgtggcaat tatgtacatc
     2161 gcatctcgcg cgccgctcgg caaatcttcc attttgttcg atacgatgcg cgggcttctc
     2221 cttgcgactt cacggccacc gccgcctcca ccggcagtgc ctacactcgt gcctgcggtt
     2281 tcctccatct gcgctttgac ctcttcgggg tctgccagct tcagcttctc gcgcaggtga
     2341 tagtcgttca cgtcttcgtc gcgatgcgcg tagaagatgc tcaacggatc ggcgagactg
     2401 taagccgtga gcgcgagcac ggattcgtca acgacgacga ccgccgtgtc cgtgcctgcg
     2461 acagcacggc ctgcggcgtc cttcacttca acctcaacga gcgtctcagc ccccggttca
     2521 agcaccgcct cgcgcggcgt ggccttcacg ctcagacgcc gcgacgcggg tggaatgtcc
     2581 agattgatct cgcccgaagc gtaggccggg cgtttcggca cattcgccaa ctcgacgccc
     2641 gcatcgttta cacgcgttgc cgcgcccacg agatcgacct gcacgttgac gttcggcgtc
     2701 atcccgtctt cgagcgggat gcgcagcgtg taggaactct cattcatcgt gaagcgttcg
     2761 gtgcggagca ggccggaacg cctgagcgtc aggacgccct ccgcaggggc aaaaggcgac
     2821 tgcaagagaa tctccgccgt ctccccgccc gcataacttt tccggtcggg gatcagttcg
     2881 acccgctcct gcgcgacctc gcgcagcggc ggcctcttcc cgcccgcgac ccacagcgac
     2941 aactcgctct cgttcaaacg ctcgcgctcg tcgcgcacac gcgccgtcag gcgatacatg
     3001 ccgccctcct tcgccggaaa acgcacgcgc acggcctccg cgccgctctt gatcttctgc
     3061 tcctgcgcct cctgttcacg cggctgccag gagcctgcct cgtaaacgta ttcgagtcgc
     3121 acgagtcgta aattcacgtc gcggttcggg agcgcgcgcc cgtctagatc ggtgacgatg
     3181 gccgagatgt cgaaggcttc gcccttctgc acgaacgttc gcgccgagcg cagcccgacg
     3241 tagacatccg ccgggtggac gagcagcgtc gccgtcgaag cgaacgtctg gcggttgaca
     3301 tcctgcacgc gcgcttgggc tgtgacgctc gaagggcgcg ccggttgcac cccttcaaaa
     3361 tcgatccgca acgtgtgctt gccttccgcg tcggtgcgcc cttgaaggtg ttttgtgttc
     3421 cggtcgccgc tcatccggtc gctgcgccac cagggataga acgtcccgaa cgtgtaatcg
     3481 tcacgaccgg gcggcgtgta gcttgtcggc tttgaagaga cagtccagtt gacctccgtg
     3541 tcgggcaaac tgccgcccgc gaaataggct gcggcggtgg ttaccgtcgc gtgtgttccg
     3601 acgaagtgtg gggcttccga agcgcgcgcc gtgagttcga attcggggcg gcgaaattcc
     3661 tgcacctgaa attgatgtat gtgcgcgccg ctcccgtcgg cgaaggtgaa ggcgaggtgc
     3721 gcttcgccga gattcatcgt cgcggggagt tgcagtttga ggttgaatcc ggcgagggca
     3781 ttgagtttca cgctgccctt cgtgatctcg ttgccctgtg catctctcaa gacgtagtgg
     3841 gcgagttcgc ccgccgcccc ctgcaacatc tccgtgtcgc cggttgcggt caggtcaact
     3901 ttgcgaatcc agcctttgac gttcacctct tcgccgggac ggtacagctt gcggtcgtca
     3961 aagatgtacc agcgcagcgt ctcgctcgaa tcgtaacgac gccaactgct gctcctgtcg
     4021 tcatagttat aggaagagta ccgctgtggc aggatcgcta tgtcgtcgcc acgccgcgcg
     4081 acgagcagcg tactctgctg cgccttcgga tcgaaggtgg ctttgaacgg gaggcgcgcg
     4141 aggccgtctg ccccggtcgt gcccgtcagg ccgtcgggcg agactgtcaa ctccactccg
     4201 gccagaggct ttccgtccgt aagcgagtta gcccacgcga cgagttcgcg tttgtctgcg
     4261 aaaacgtcca gcccgatgcc ggtcgcctgc acccatgctt cggcggagtt ctgccgcccg
     4321 gcgtacgcgt tcgcacgcgg cttcctgccc gcagaagagg ccacgacggg ttcgacgcgg
     4381 acgaaaacct gcccgtaccc gttcgtcagt gcgggcgtga gatcaataga ggtttcggtg
     4441 agttcgtccg ccttcgcttt gacttcgagc acttcgtcga agacaagtcg ccccggcggc
     4501 gtgtgcgctc cattccggcg cgcgctcccg gcctggtagc gacgaaactg cggccagtcg
     4561 gcgggcgtga ctttgtagag catgaccttg agcttcggat agttgacgct gtagaccgtg
     4621 aagccgcgcc tgcccgccgg atcgagcacg gcgaagctct cgcccgtggc gaagagacgc
     4681 ggcgtggccg tagtgatctt gaaggtgaag acgttcgtgc ccgtgagcgt ctgccggaac
     4741 gtgtcggaga cggcgcggtc gagcgtgacg gtgtagaccg tgttgctgcg cttcgcgcct
     4801 tcgatgaaga gcgagttgtg gcggacgctg atcttcgcgt ccggcatgcc gggcttgatc
     4861 gtgacctgcg ccgggctgaa tttcgccgcg tcgagttggt tgttgaacgt gaggatcaac
     4921 tggtcgaagg gcgagcatct ttgcctgtag ccgcaaaagg cttcggtgac gcgcagcgcg
     4981 ccgtaagtct taaaggcgaa actctgctct ttgagggtcg tgcgcgcgcc ttcggcggag
     5041 ggcgtgcccg gcgggacgac gatcctgatg ttggtgtcag gtggaagcgc gtttttcgtc
     5101 gctccgtccg cgctcacggc acggacggcg agccagcgtc ccgcctgcgc ctgtttgctg
     5161 aaataactga cggacggatc agcggcgatc tcttcctgcg tcgcaaggcg caggcgcgcg
     5221 cccgtggcga cgggttgcag cctgagccgt tcgagcacgc gtgccgcatc aatgttctgg
     5281 tcgaactcaa ggaacatcag cgaatcgcgc ggttcgcttg ggccgccggg gtgagaattt
     5341 ttcagcgtcg ggggcggcgt ggcaaaacta aaagtctcgg ctaccgcgag cgcgttgccg
     5401 agcgcggact tcgttccggc gggaacggtg acggtgtagt tggtcgccat cggcagcctg
     5461 ccgccttcaa ctttgggctg gaagatgagt gtctgcgtgc cgagccaacg ccagacgccc
     5521 tccggttggg gcttcatcct gacggggacg ctcgccgccg tctcctcctg cgatgaggcc
     5581 gcgaccatcg gttgtgaaaa cgtgatgctg aacgcgggag ccaaagcgac ttcgccttcc
     5641 ggcgcgaagc gcagaacttc gagcggcgcg cgtgtatgcg tggggacggg cggcgcgttc
     5701 ggggcgggcg gggcgaatgc tgcctgaatg gtttcgcctg cgcggggcgg cggcaacgaa
     5761 cgctcgcgga gtttgaaatc ctgcgtgtcg ttcgcctccg gtgtgaggtt gggcaggcgg
     5821 tcgagcagtt tttttgtctc ggctgcggac aacaactcgg cgggcgcgag cgtctcgggc
     5881 gcggggcgtt caacctgttc cctgccttcg ctcaggcgga aacgcaggcc gttttgcgtc
     5941 gcggcctcca ccgtctcctg cccgccccgc gtagcggcga cgcctgcagg cggcggggca
     6001 aggagcgtga aaatgatcgt caatgccaac aggcgtgtgg gggcgtgctt gattttcatg
     6061 cgagttaatt gcctctgttt gatcggctag attgatcgtc gtgtgcgatt gggagcaacc
     6121 gttgaacgcg tagacagcag ggttcttgcg cgatgcgcgg cgaaccgcgc cggacgactg
     6181 gccgcgtgcg agacgccgcg ccggtctagg tcgctccgac cggaggtgcc tgcgtaagct
     6241 atcagatgga gatgtgcgcc gtctgcgggc gcgtctttca gtcgcgtcag atatgaaggg
     6301 cttccggttg aagaggttgt caggccatag agagattttc tgaaagcgcg tcggctgcat
     6361 gccggtggcc gaacccgcgc ccgcgccgga atgctgaaag aagagataga actgaatgtt
     6421 ctccccctgc ggtctgcacg aacccgacgg cgcaatccta tattaccgcc agggcgggca
     6481 gggaatgaaa ctcacgccac actgaattct ctcaaaacca cttcgacacc tgagtaaaag
     6541 atgtccggca gaggacggcg cggcgcatga acctcgaatc atttcgaacg aggtgagacg
     6601 ggcagacgcg tctggccgga gcgcagcccg gcgcgcgtgc gcgaagtgcg cgccaccccg
     6661 cacactctgt gcggcacggg tgcgtaaacg tacagacggc ctgtgcatgg gcttcgtata
     6721 gtctgttttc ccttatgtgt aatctttaaa ggagatgcgg gttagctgtc gccgaccggc
     6781 atcgtgttgc gtcttgaatc gcgggttctg atgcccggct tttacgctcc tgagcgcgga
     6841 accgtgcctg cgggtttaag gcacacgcgc tgccgcaccg gcgcagataa ccgtgaagtg
     6901 aggctttacg atggaagcac attcaaccga acgaatcacc gtgagagatt tcaggcaact
     6961 actcgaatcc ggacgccacc cgaaattgaa ctgcccggct tgcgggcagt cgtggctcat
     7021 cgtcggcgtc cggcggcacg agacgcatgc gtgcaaggcg tgcggccaca ggtttcacct
     7081 cacagcctct ttgcgtgagg gtgcgggaga gggcaaatga gactcaaacc attagggcaa
     7141 caagtggtcg tcgtgttcgg cgcatcgagc ggcatcgggc gggaggctgc ccttcgattc
     7201 gccggacacg gggcgaaggt cgtggttgcg gcgcgaggtg agaaggggct tgcctctctg
     7261 gtcggcgaga tacgacaaag cgggggcgag gcggtcgccg tgcaggccga ggcgtcagac
     7321 tttgaacagg tcaagcgcgt ggcggacgtg gccgtcgaaa ggtacggcag gctcgacacg
     7381 tgggtgcatt cggcggcggt cgccctctac gcgccgttcg agcagacgac cccggcggag
     7441 tggaggcgcg tcgtagacgt gaacctcaac gggcaggctt acggcgcatg ggctgcgctc
     7501 ccgcacctgc ggcgcgaggg gcgcggcgcg ctcatccaca tctcttcggt cgaggcgctg
     7561 cgcgcgcttc cactgcacag cgcgtatgcg gcatcgaagc acggcatcaa ggctttcacc
     7621 gaagctctgc gcgtcgagtt gcagcacgac cgggcgcagg tcagcgtcac gaacatcatg
     7681 cccgcttcaa tcaacacgcc gctcttcaac aaggcgcgca ccaagatcgg ctacaagccg
     7741 atgggactgc cgcccatcta cacgcccgcg attgtcgcca aagcgattct ccacgcggcg
     7801 caaaatccgg tgcgcgaaat gatctgcggt ggctccggct ggatgatcaa gacgggcgag
     7861 caactcgccc cgcatttgat ggataaggtg ctcaggcacg ccggtttcga gtggcagcgg
     7921 acgaacgagc cgaaatccga aaacgagccg gacaatctct ttcatccggt cgaaggtgat
     7981 gaccgcgtaa gcggcgactt cacttaccaa acgatgccga agagcatctg gacgtggtgg
     8041 gaacgccacc cctctgcggg caatctcatc gcgggggctg cgctcggtgc tttagctttg
     8101 acggcggcgc gccgcttcgg tagcgcggat ggcggcagtc gtgcgcgcag tagtgccgac
     8161 tcacgcgctg cggcggcctc ttcgacagcg ggcggataaa acgtctgagc ggtgcttctg
     8221 acgatgcctt gatcgttcac ctgcgacttg cggcgtggta gcggagatgg tgtcgccggt
     8281 ggggaacagg gtagagataa agcagggtag agataaagga tgagtctgaa ccgtccgcgt
     8341 gacttcatcc tcgacgctta acggatcgag cagacggtgg tgatgaaatg cagataactg
     8401 aagtgcctgg caagctgtac gtgatcggcg ggaaggtgga cagggaaggc gaccgcacga
     8461 tcctcaggaa gttcgtgcgc gatgccggag gggcggaggc gcgcatcgtc gtgatgacgg
     8521 tggcgaccga cgagccgcag gagtccggcg acgactaccg gagagccttt caaggactgg
     8581 gcgtgagcga catccgggtc gttgatgtct cgcatcgaaa agatgccttc gagccggaga
     8641 gcctccgcac catcgagcgc gccacagccg tcttcttcac cggcggcgat caactccacg
     8701 tgacgagttt gatcggcggc acgccgatgc aggaggtgct ctaccgccgt caccgggagg
     8761 ggttcgtcct cggcggcacg agcgcgggcg cgaccatgat gtccaacacc atgatcatcc
     8821 gtggcgactc gaaccgcaat ccacgcttcg gcgcaataga catcgctccc ggcatggact
     8881 tcgtgcccaa caccatcatc gatacgcatt tctcacagcg cggtcggctc ggtcgtctgc
     8941 tgacggcagt agcacactac ccgcaagacc tcggattggg cattgacgaa aacactgcgt
     9001 tggtcgtctc cggtctacag ttcgaagtga tcggggagaa ctccgtcacg gtggcggacg
     9061 gcggaggcat gacctacacg acgctgcccg acatcgaacc caatgagagc ctttcgctgc
     9121 acggcgtaaa gatacacgtg ctggcggcgg gtcaggggtt cgacctgtcg gagcgtatgc
     9181 caatcgggga actacccgaa cagtcgccgg agaagtcgag gtcaacaacc gcttccgcag
     9241 tgggcaagtc tgcggcacga atgaaaatcg agagcgtgcg cacgctcgcc ggggcgaacg
     9301 tctatagcca ccgccccgtc ctcgtcatga ggctcgacct cggggagttg aacgagaaag
     9361 agagttacga aatccccggc ttcattgatc gtctgctcgc ggggctgccc ggcctgcgta
     9421 cacatcattg cagcaaaggc aggccgggcg gatttgtcga acgtctccac gagggcactt
     9481 acttcggcca cacggtcgaa cacgtcgcgc tcgaactggc gtcgctcgcg gggtgcagcg
     9541 cgacccacgg aaagacacgc tacgcggggg agcgcggcat ctacaacgtc gtcatcgagt
     9601 actgcgccga gcaggccacg cgctatcttt tggagacagc cgtggagttc gtcgaggcgt
     9661 tgatcgcggg cgaagagttt cccgtgcaag agagaattga agaggcgaag cggactatgg
     9721 cggacacgga gttggggcct tcgacccgcg ccatcgtcga ggccgccgaa cgacgcggca
     9781 ttccctggat tcgtgagaat gaagcgagcc tcatccaact cggccacggg aagaacctca
     9841 ggctcattca ggcggccatg acggac
//
LOCUS       DASWNS010000147         6535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_395076, whole genome shotgun sequence.
ACCESSION   DASWNS010000147 DASWNS010000000
VERSION     DASWNS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395076"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VGB76_04980"
     CDS             complement(<1..607)
                     /locus_tag="VGB76_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0098289.1"
                     /translation="MRSLRHSNGMAEQLYDCIVIGAGPAGLSAALFLARYRRRVLTFH
                     HNSPRNIYSHGVHGFLGHHGIQPVELLARGREEVTQHGGLIVEGCVTKIERLGKECFR
                     VQTGDEKIVGQSFDTRRILLATGLRDFMPECPGFREFYGVSVHHCPDCDGFEATGKRI
                     AVVSHGKNGVGFTLGLLTWTDRLTLITDGNAGDLTDEHRAKL"
     gene            complement(739..1224)
                     /locus_tag="VGB76_04985"
     CDS             complement(739..1224)
                     /locus_tag="VGB76_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY0098290.1"
                     /translation="MDHVYLAPSRISGTGVFTARRLRADEFVLSFTADAAEFVTYAGT
                     VRFPEQEGNYVQVAADAYVLPSPPSLYLNHSCDPNTGVRHATEIVALTQVEAGDELTF
                     DYSTSMAEDGWEMDCACGSAICRGRIRDFRHLPLERQLYYIERNVVGDFCLATLTAHA
                     L"
     gene            1396..2325
                     /locus_tag="VGB76_04990"
     CDS             1396..2325
                     /locus_tag="VGB76_04990"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY0098291.1"
                     /translation="MTKHSAARLVLSLGALALLVCALYGFALKRPDMQAPDTNTSDAG
                     EALPARRTLAMAHRGGAGLWPENTAYAFAQSVALGVDVLEMDLHGTADGALVVIHDAT
                     VERTTNGAGRVNNLTLAALKKLDAGYRWTNDGGRTFPFRGKGITVPTLLEVLDEFPRA
                     RLNIDIKQAQPSLVKSFCRTLGERGAKGRVTVASFNSGTLEEFRRECPEVSTSAGMDE
                     VIALASALQSGRRIELGKSHPRLLQVPESAFGRGWLTPELVAEAHRAGLEVHVWTVNE
                     EAGMRRLIALGVDGIMTDYPDKLVALLKQAAGH"
     gene            2344..3276
                     /locus_tag="VGB76_04995"
     CDS             2344..3276
                     /locus_tag="VGB76_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0098292.1"
                     /translation="MSFETDAGGVKLMEKRVLGRTGMHVSVLGFGGSEIGYGQAAVET
                     VERLLGDALDAGLNVIDTAECYVTSEELIGRAVSHRRADYYLFTKCGHAAGINLPDWD
                     ARLLEQSIERSLRRLRTDHVDLLQLHSCSDEVLRQGDVIEVLQRAREAGRTRYIGYSG
                     DGLAALHAVECGAFDTLQTSVNIADQAALEVTLPNARAHGLGVIAKRPLANAVWKHTE
                     RPGDPYVQPYWDRLQKLDYQFLRGDMAEAVATALRFTLSVPGVHTAIVGTEKPERWRQ
                     NAAMLSAGALDAAQFDAILTRWAERAEADWEGEV"
     gene            3362..4912
                     /locus_tag="VGB76_05000"
     CDS             3362..4912
                     /locus_tag="VGB76_05000"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY0098293.1"
                     /translation="MQEPNVNGSGDAAPEHAPRWGKVIDQTRCIGCHACTTACKSENL
                     VPLSVTRTYVKHVDVGEYPQTRRAHQVTRCNQCTHAPCATACPTTAMFKRPDGIVDFD
                     KSICIGCKACMAACPYDAIFINPEDHSAEKCNFCAHRIDMGLEPACVVVCPVEAILVG
                     DMHDAGSKVARIVNRDPVNVRRPEKETLPKLFYKGAHQATLDPLAARRPDGELFMWSE
                     QRTGRDFVVSGHPDYNNTSAAALLSYDVAHTIPWDWRVSLYTWTKGIAAGAYLVPLLL
                     VVLGFIGTGSPLWLWVAPVVAGAFLAFTGLLLIWDLEHPERFYLIFTRPQWRSWLVKG
                     AFIIAGYSLVLALHFAASLFGATTPQRFLMIAGLPLASLTAIYTAYLFAQAKARDMWQ
                     NPLLPPHLFVQSLLVGAGALLPFAAWLERGAVAPLSWSLGVLSLIHLLCIWGETSVTH
                     PTAHAHLAVTEMTRGRYRTFFWTGIVLTLLGGLAPWFGLASVPFALAGLLLFEHAYVQ
                     AGQSVPLA"
     gene            5025..5489
                     /locus_tag="VGB76_05005"
     CDS             5025..5489
                     /locus_tag="VGB76_05005"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY0098294.1"
                     /translation="MMATKRFRALLEKHENSEATGITIPFDVQQTYGTRARVPVRGTI
                     NGFPYRGTIFPVGGKYIFVVNRELRTNANAKGGEMISVTMERDDEPRLITPPADLARA
                     LKAAPAAQAAWDKLSSTHRKEHARAVEDAKRPETRVRRIEKAIAQLVEGKKP"
     gene            5559..>6535
                     /locus_tag="VGB76_05010"
     CDS             5559..>6535
                     /locus_tag="VGB76_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0098295.1"
                     /translation="MKLSELNQRVSAARRETEARGETFYPGASRVHLAAFPPKERWDD
                     WVELDSRAWPERKERRYMLVPTTCFNCESACGLLAYVDRESLQVQKFEGNPEHPGSRG
                     RNCAKGPATLNQITDPDRILYPLKRVGARGEGKWERVSWDEALDAIAARIRKAIIERR
                     HNEVMYHVGRPGEDGFTERILAAWGVDGHNSHTNVCSSSSRAGYQYWMGLDRPSPDHA
                     NAKVIFLISSHLEAGHYFNPHAQRVIEAKAEGAKLIVFDTRLSNTATHADYFVSPYPG
                     SEAAIVLSIANYLITNNLYNAEYVRRWWNWEEFMAVEHPERETNFENFQT"
BASE COUNT         1226 a         2049 c         2108 g         1152 t
ORIGIN      
        1 ccaacttcgc gcggtgctcg tccgtcaggt cgcccgcgtt gccgtcggtg atgagcgtca
       61 acctgtccgt ccacgtcaag agtccgagcg tgaagccgac cccgttcttg ccgtgcgaga
      121 cgacggcaat gcgtttgccc gtcgcttcaa agccgtcgca gtccgggcag tgatgtacgg
      181 agacgccgta gaactcgcgg aagcccggac actccggcat gaaatcgcgc aagcccgtgg
      241 cgagcaggat gcggcgcgtg tcgaacgatt gacccacgat cttttcgtcg cccgtctgga
      301 cgcgaaaaca ttctttgccg aggcgttcga tcttcgtgac gcagccttcg acgatcaaac
      361 cgccgtgctg cgttacctct tcgcgcccgc gcgccagcag ttcgacgggc tggatgccgt
      421 gatggccgag gaagccgtga acgccgtgcg agtagatgtt gcgcgggctg ttgtgatgaa
      481 aggtgagcac gcgcctgcga taacgcgcga gaaagagcgc agccgagagt cccgcaggtc
      541 ccgcgccgat tacgatgcaa tcgtagagtt gttccgccat gccgtttgag tggcgcaaac
      601 tacgcacacg cgtctgcgcc tcgccccctt gttttgcact tgaaaatgta tcttccggtt
      661 cttagtcgaa gtcaaacacg gcgacggggg gcgcgtcaac gcggcgtcgt gcggcgacct
      721 tcgcggcagc acgcgcgact agagcgcgtg agcagtgagc gtggcgaggc agaaatcgcc
      781 gacaacgttg cgctcgatgt agtagagctg cctttcgagg ggcaggtgcc ggaagtctct
      841 gatgcgcccg cgacagatgg ccgagccgca ggcgcaatcc atctcccaac cgtcctcggc
      901 catcgaggtc gaatagtcga acgtcagttc gtcgcccgct tcgacctgtg tgagcgcgac
      961 gatttcggtc gcgtggcgga cgcccgtgtt cgggtcgcag gaatggttga gatagagcga
     1021 gggcggagag ggaaggacgt aggcgtccgc cgcgacctgc acgtagtttc cctcctgttc
     1081 ggggaagcgc acggtgcctg cataagtgac gaactccgcc gcgtccgccg tgaaggagag
     1141 gacgaactcg tctgcgcgga gacggcgcgc ggtgaagact ccggttccgc tgatgcgcga
     1201 cggcgcgaga tagacatgat ccatataact gctatcgctc cggctcgcgc gtcggaatta
     1261 ggatgcgcgc cgctggtcaa actgcaccgg gctggtgcta aacttgcagc ctcaggatat
     1321 aaaccattaa ctcgcgtcgt ctgtcctgct acgtacagac gacgcgccgg ggcgatgcga
     1381 gggagttggc gagaagtgac gaaacatagt gcggcaaggc tcgtgttatc gctcggcgcg
     1441 cttgcgctgc tcgtctgcgc gctctacgga ttcgcgctaa agaggccgga catgcaagca
     1501 cccgacacga acacttccga cgccggagag gcactccctg cgcgccgcac actcgcgatg
     1561 gcgcatcgcg gcggcgcggg actctggccg gagaacactg cttacgcctt cgctcaatcc
     1621 gtcgcgctcg gcgtggacgt gttggagatg gacctgcacg gcacagccga cggcgcgctc
     1681 gtcgtcatcc acgatgcgac ggttgaacgc acgacgaacg gcgcagggcg cgtcaacaac
     1741 ttgacgctcg ccgcgctgaa aaaactggat gcgggttatc gctggacgaa cgacggcggg
     1801 cgcacgtttc ccttcagggg caaagggatc accgtgccga cgctgctcga agtgctcgac
     1861 gaatttccgc gtgcgcgtct gaatatagac atcaaacagg cgcagccctc gctcgtcaaa
     1921 tccttttgcc ggacgctcgg cgaacgtggc gcgaagggca gagtgacggt cgcttccttc
     1981 aattccggga cgctcgaaga gttccggcgc gagtgtccgg aagtgagcac gtcggctggc
     2041 atggatgagg ttatcgcgct ggcgtctgcc ttgcagtccg gacggcgcat tgagttgggc
     2101 aagtcgcacc cgcgcctcct gcaagtgccg gagagcgcgt tcgggcgcgg gtggctgacg
     2161 ccggaactcg tcgcagaggc gcatcgcgca gggctggaag tgcacgtctg gacggtcaac
     2221 gaagaggccg ggatgcgtcg gctgatcgcg ctcggcgtgg acggcatcat gacggactac
     2281 ccggacaaac tagtcgcgct gttgaagcag gcggcagggc attgagttga atttcatacc
     2341 gggttgagtt tcgagacgga cgcgggcggg gtgaaactga tggagaaaag agtgctggga
     2401 agaacgggca tgcacgtgag cgtgctcggc ttcggcggtt cggagatcgg ctacgggcag
     2461 gccgccgtcg agacggtcga gcgtctgctc ggcgacgcgc tcgatgccgg actcaacgtg
     2521 atcgacacgg cggagtgcta cgtcacgagc gaggagttga ttggtcgcgc cgtctctcac
     2581 cgccgcgccg attattacct cttcacgaag tgcggccacg ccgccgggat caatctcccc
     2641 gactgggacg cgcgccttct ggagcagagc atcgaacgca gcctcaggcg tctgcgcacg
     2701 gatcacgtag acctgttgca actgcacagt tgctcggacg aggtgttgcg gcagggcgac
     2761 gtgatcgaag ttctgcaacg cgcacgcgag gccggacgga cgcgctacat aggctatagc
     2821 ggcgacggcc tcgccgcgct gcacgcggtt gaatgcggcg cgttcgacac gttgcaaacc
     2881 tccgtcaaca tcgcagatca ggccgcgctc gaagtcaccc tgccgaatgc gcgcgcacac
     2941 ggcctcggcg tcatcgccaa acgccccctc gcaaacgccg tctggaaaca cacggagcgt
     3001 ccgggcgacc cctacgtcca accctactgg gatcgcctgc aaaaactcga ctaccaattt
     3061 ttacgcggcg acatggcgga agcagtggcg acggccttgc gttttactct gagcgtgccc
     3121 ggcgtgcata ccgcaatcgt cgggacggag aagccggagc gttggcggca gaatgctgcg
     3181 atgctctcgg cgggtgcgct cgacgccgca cagttcgatg cgattctcac tcgctgggcg
     3241 gagcgcgcgg aagcggattg ggagggagaa gtttgagccg cacgccgcgt tgtgcagtcg
     3301 ggcgcgtttc ggtgtagtct gtcgtttaga tcagcgttaa gccatcctgt cggagatatt
     3361 gatgcaagag ccaaacgtga acggaagcgg ggacgcagcg ccggagcacg cgcctcgctg
     3421 gggtaaagtc atagaccaaa cgcgctgcat cggttgccac gcctgcacga ccgcctgcaa
     3481 gtcagagaac ctcgtgccgc tctccgtgac gcgcacgtac gtgaagcacg tggatgtcgg
     3541 cgagtatccg cagacgcggc gggcgcatca ggtgacgcgc tgcaaccagt gtacgcacgc
     3601 gccctgcgcg acggcctgcc ccaccaccgc gatgttcaaa cgccctgacg gcatcgtcga
     3661 tttcgataaa tccatctgca tcggctgcaa ggcttgcatg gccgcctgcc cctacgatgc
     3721 gatcttcatc aaccccgaag accactcggc ggagaagtgc aacttctgcg cgcatcgcat
     3781 cgacatggga ctcgaaccgg cctgcgtcgt cgtctgcccg gtcgaagcca tcctcgtcgg
     3841 cgacatgcac gacgcgggtt ccaaagtcgc gcgcatcgtc aaccgcgacc cggtgaacgt
     3901 caggcgaccg gagaaggaga cgctgcccaa acttttctac aagggcgcgc atcaggccac
     3961 gctcgacccg ctcgccgcgc gtcgccccga cggcgaactc ttcatgtgga gcgagcagcg
     4021 cacgggcaga gacttcgtcg tctccggcca tcccgattac aacaacactt ccgccgccgc
     4081 actgctctcc tacgatgtgg cgcacacgat cccctgggat tggcgcgtca gcctctacac
     4141 ctggacgaag ggcatcgcgg cgggcgcgta tctcgtgccg ctcctgctcg tcgtgctcgg
     4201 cttcatcgga acgggcagcc cgctctggct ctgggtcgcg cccgtggtcg cgggcgcgtt
     4261 tctagccttc acaggtctgc tgctcatctg ggacctggaa caccccgaac gcttctacct
     4321 gatcttcacg cgaccgcaat ggcgcagttg gctcgtcaaa ggcgcgttca tcatcgcggg
     4381 ttattcgctc gtcctcgccc tgcacttcgc cgcgagtctc ttcggcgcga cgacgccgca
     4441 acgcttcctg atgatcgcag gactgccgct cgcatccctg accgccatct acacggccta
     4501 tctcttcgcg caggcgaagg cgcgcgacat gtggcagaac cccctgctgc cgccgcacct
     4561 cttcgtacag tcgttgctcg tcggcgcggg cgcgctcctg ccgttcgccg cctggctcga
     4621 acgcggagcg gtcgcgccgc tttcgtggtc gctcggtgtg ttgagtctca tacacctgct
     4681 ctgcatctgg ggcgagacga gcgtgacgca cccgacggcg cacgcgcacc tcgccgtcac
     4741 ggagatgacg cgcgggcgtt atcgcacttt cttctggacg ggcatcgtcc tgacgctgct
     4801 cggcggcctc gcgccgtggt tcggcctcgc ctccgtgccc ttcgcgctcg ccggactgct
     4861 cctgttcgag cacgcctacg tgcaggccgg gcaatccgtc ccgctggcct gagacggagg
     4921 caggcacgaa ttgaagatca cgtgtggatt gatgtagcac ccgccgccgc gacacgaaac
     4981 gattgcctcg cgcgcaggct ctggcattaa accggatgga gaagatgatg gcgaccaaac
     5041 gtttccgcgc gctgctcgaa aaacatgaga acagtgaagc caccggcatc accattccct
     5101 tcgacgtgca gcagacctac ggcacgcgcg cacgcgtccc cgtgcgcggc acgatcaacg
     5161 gcttccccta tcgcggcacg atcttccccg tgggcggcaa atacatcttc gtcgtcaacc
     5221 gtgaactccg cacgaacgcc aacgccaaag gcggcgagat gatcagcgtc acgatggaac
     5281 gcgacgacga gccgcgcctc atcaccccgc ccgccgacct cgcacgcgcc ctcaaagccg
     5341 cccccgccgc gcaggccgcc tgggacaaac tcagctccac gcaccggaag gaacacgccc
     5401 gcgccgtcga agacgccaaa cgccccgaaa cgcgcgtccg tcgcatcgag aaggcaatcg
     5461 ctcaactcgt cgaagggaaa aagccgtaac accgtaacac cgtttatcag ttagagggag
     5521 catctgcgtt aaagtggatc gtgaatgaag ttgaacttat gaaactgagt gaattgaatc
     5581 agagggtgag cgcggcgcgc agggagacgg aggcgcgcgg ggagacgttt tatccggggg
     5641 cgagccgggt gcatctggcg gcgtttccgc cgaaggagcg ttgggacgat tgggtggaac
     5701 tggattcgcg cgcgtggccg gagcgcaagg agcgtcgcta catgctcgtg ccgacgacct
     5761 gtttcaactg cgagtcggcg tgtggcctgt tggcttacgt tgaccgcgag agcttgcagg
     5821 tgcagaagtt tgagggcaac ccggaacatc ccggttcgcg cgggcgcaac tgcgcgaagg
     5881 gtcctgcgac gctcaatcag atcaccgatc ctgatcgcat actctaccct ttgaagcgcg
     5941 ttggtgcgcg cggcgagggt aagtgggagc gcgtcagttg ggacgaggct ctggatgcca
     6001 tcgcggcgcg catccgcaag gcgattatcg agcggcggca caacgaggtg atgtatcacg
     6061 tcgggcgtcc gggcgaggac ggcttcaccg aacgcattct cgcggcgtgg ggcgtggacg
     6121 ggcacaactc gcacacgaac gtctgttcat cttcgagccg cgccggttat caatactgga
     6181 tggggctgga caggccgagt ccggatcacg cgaacgcgaa ggtcatcttc ctgatcagtt
     6241 cgcacctcga agcgggacat tatttcaacc cgcacgcgca acgcgtcatc gaggcgaagg
     6301 cggagggcgc gaagctcatc gtcttcgaca cgcgtttgtc gaacacggcg acgcatgcgg
     6361 actatttcgt ctcgccctat cccggctccg aagccgccat cgtgctctcg attgcgaatt
     6421 acctgattac gaacaatctc tataacgcgg agtacgtgcg ccgctggtgg aactgggaag
     6481 agttcatggc ggtggagcat ccggagcggg agacgaactt cgagaatttt cagac
//
LOCUS       DASWNS010000148         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_447284, whole genome shotgun sequence.
ACCESSION   DASWNS010000148 DASWNS010000000
VERSION     DASWNS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447284"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VGB76_05015"
     CDS             <1..181
                     /locus_tag="VGB76_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098296.1"
                     /translation="GASFLFYGSITLIVEARLAVASLRIEMDFTWRLGKHYAPAELAA
                     RHNSPHYRLRVKPRP"
     gene            178..1218
                     /locus_tag="VGB76_05020"
     CDS             178..1218
                     /locus_tag="VGB76_05020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0098297.1"
                     /translation="MNERLKPSYLALFETGELRARVEALESLLARCTVCPRDCFNNRL
                     EDEIAACYSGRLPIVSSYTAHFGEEPALTGTRGAGNIFFGNCNLRCVYCQNYQISQTH
                     REQLKNEVTHERLAGMMLELQTRGCHNINFVSPTHFAPQLARAVLIAAGQGLRLPIVY
                     NTNAYDSVEVLRLLDGIVDVYLPDLKYADSADGYAYSKVRDYTEHARRAVAEMFRQTG
                     DELIFDEEAGLLKRGLVIRLLVLPNDLAGIRETLGWIRDALSPRVAVSLMAQYYATNR
                     AATDERYTLLSRRITENEWLRAVSALEELGMEEGWMQEYDGASHYYRPDFTDSETPFK
                     DIRDFRTATKEQ"
     gene            1290..1490
                     /locus_tag="VGB76_05025"
     CDS             1290..1490
                     /locus_tag="VGB76_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098298.1"
                     /translation="MTSSFISAAFFDNTAPGTAGWVVLAVWFVLFVLVLNTIIFFLRR
                     LFRRWHGRVEQAPQPTDREGMT"
     gene            1487..1669
                     /locus_tag="VGB76_05030"
     CDS             1487..1669
                     /locus_tag="VGB76_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098299.1"
                     /translation="MSASTASLAYLHGLEAIIIVCVVGLLGGVLFAVILAFILRSFLH
                     RRQLTTPEVNRRERNI"
     gene            1669..2157
                     /locus_tag="VGB76_05035"
     CDS             1669..2157
                     /locus_tag="VGB76_05035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0098300.1"
                     /translation="MSSTKTIAGVGTIAPDFILHDTEGAEWQMSAQRGRVVVLLFYPG
                     DETPVCTKQLCSVRDNWESYAATGAEVVGISTDTVESHQKFTEHHNLPLRLLADTDGK
                     VHKAYNASSWFPGRSARAVFVVDAKGIIRYRKVETVSLFRPRDEEIIEAIRAAQQQQQ
                     QS"
BASE COUNT          479 a          765 c          693 g          456 t
ORIGIN      
        1 gggcgcgtcg tttctcttct acgggagcat cacactgatc gtcgaagcgc gcctcgccgt
       61 cgcttctctg cgcatcgaga tggatttcac ctggcgactc ggcaagcact acgcgcccgc
      121 cgaactcgcc gcgcgacaca acagtccgca ctatcgcctg cgcgtcaaac cacgaccatg
      181 aacgaacgcc ttaagccttc atatctcgcg ctcttcgaga cgggcgaact ccgcgcccgc
      241 gtcgaggctc tggagtctct gctcgcgcgc tgcaccgtct gcccgcgcga ttgcttcaac
      301 aaccgtctgg aggatgagat cgccgcctgc tattcgggac ggctccccat cgtctcttcc
      361 tacaccgcgc acttcggcga agaacccgcg ctcacgggca cgcgcggcgc aggcaacatc
      421 ttcttcggca actgcaacct gcgctgcgtc tattgccaga actatcaaat ctcgcagacg
      481 caccgcgagc aactcaagaa cgaggtcacg cacgaacggc tcgccgggat gatgctcgaa
      541 ctacaaacgc gcggctgcca caacatcaac ttcgtctcgc ccacgcactt cgccccgcag
      601 ttggcgcgcg ccgtcctcat cgccgccggg cagggcttgc gcctccccat cgtctacaac
      661 acgaacgctt acgactcggt cgaagtgctg cgactgctgg acggcatcgt tgacgtgtat
      721 ctgcccgatc tcaagtatgc ggactccgca gacggctacg cttactccaa agttcgagac
      781 tacacggagc acgcgcgccg cgccgtcgcg gagatgtttc gccagacggg cgacgagttg
      841 atcttcgacg aagaagcagg cttgctcaaa cgcggcctcg tcatccgcct gctcgtcttg
      901 ccgaacgatc tcgcgggcat ccgcgaaacg ctcggttgga tcagggacgc gctcagcccg
      961 cgcgtcgccg tctcgctgat ggcgcaatac tacgcgacga accgcgccgc caccgacgaa
     1021 cgctacacgc tgctctcgcg tcgcatcacc gaaaacgaat ggctgcgcgc cgtctccgcc
     1081 ctcgaagaac tcggcatgga agagggctgg atgcaggagt acgacggcgc atcgcactac
     1141 tatcgccccg actttaccga cagcgagacg cccttcaaag acatcagaga tttcaggaca
     1201 gcgacgaaag aacagtgacg agtgacaagt gacgagttgg aacaagtgac gaatgacgaa
     1261 cgacaagtga cgggttagta aacggacgca tgacaagctc ttttatctct gccgccttct
     1321 tcgacaacac agcccccggc acggctggct gggtcgtcct cgccgtgtgg ttcgtcctgt
     1381 tcgttcttgt cctcaatacg atcatcttct ttctgcgtcg tctcttccga cgctggcacg
     1441 gcagagttga gcaggctccg cagccgactg accgggaggg aatgacatga gcgcttcaac
     1501 ggcgtcgtta gcttaccttc acggccttga ggcgataatt attgtttgcg tcgtcgggct
     1561 gctcggtggc gtgctctttg cagttattct cgctttcatt ctcaggagct ttttacatcg
     1621 caggcagttg acgacgccgg aggtgaaccg gcgggaacgg aatatttgat gagtagcaca
     1681 aagacaatcg ccggggtcgg cacgattgcg cccgatttta tcctgcacga cacggagggc
     1741 gcggaatggc agatgtctgc gcagcgcggg cgcgtcgtcg tgctcctgtt ttatccgggc
     1801 gacgagacgc ccgtgtgcac gaagcaactc tgctccgtgc gcgacaactg ggagagctac
     1861 gccgcgacgg gcgcggaggt cgtcggcatc tcgaccgata cggtcgaatc gcaccagaaa
     1921 ttcacggagc atcacaacct gcccctgcgc cttctggccg acacggacgg gaaagtgcac
     1981 aaggcttaca acgcgagttc gtggtttccc ggtcggtcgg cgcgcgcggt cttcgtcgtc
     2041 gatgccaaag gcatcattcg ttatcgcaag gtcgaaaccg tctcgctctt tcgcccgcgc
     2101 gacgaggaga tcatcgaggc gattcgcgcc gcgcagcagc agcaacagca gtcctgagcg
     2161 gcggcagcag tcttgagcgg cggcgcggac tgtcgcgcgc acgcgcatct atcatcattg
     2221 attcattgct tgaaggagaa tttgaatatg gctgacaaca tcaagtgtgc acattgcggg
     2281 gagcgtcgtc ccgcgctcgg ctacccgccg tttccgaacg agatcgggca gcgagtgggc
     2341 gcggaaatct gccaaccctg ctggcaggac tggctcaaaa aacagagcca gat
//
LOCUS       DASWNS010000149        11185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_214314, whole genome shotgun sequence.
ACCESSION   DASWNS010000149 DASWNS010000000
VERSION     DASWNS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11185
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214314"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VGB76_05040"
     CDS             complement(<1..294)
                     /locus_tag="VGB76_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098301.1"
                     /translation="MSNLQPTLPTPASAYGARFRAWNWRRALSPTGNTGDALFRGLLF
                     CCALLLVIIVGAMFVSMISNARLSLEAFGFSFLTGRSWDPIQGEFGALPFIYGT"
     gene            complement(454..1545)
                     /gene="pstS"
                     /locus_tag="VGB76_05045"
     CDS             complement(454..1545)
                     /gene="pstS"
                     /locus_tag="VGB76_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY0098302.1"
                     /translation="MLPQPRLLRATLALLLITFTTLAFGCSGQRGGTGGAAGGTIRLQ
                     GAGATFPNPLYQKWLSEYGKLNANVRIDYQSIGSGGGIKQIKEQTIDFGASDAPMKDE
                     DLRSAPGELLHIPTVLGAVVITYNLQGIAEPLRFSPEVIADIFLGKIKRWDDARIKAD
                     NPGVQLPAVDLTVVHRSDGSGTSAVFTDYLSKVSPEWKEKVGSGTSPNWPVGLGGKGN
                     EGVTGQVKQTPNTIGYVELAYAAPNRLPVALIKNSSGNFVEPSLDAVTAAAAESLPAT
                     PEDLRVSITNTAGASAYPISSYTYILAYKDQKDAAKGKALVDFLWWGIHDGERFAKDL
                     QYAPLPPQIVERAAAKINSITSGGKPLRS"
     gene            complement(1682..2098)
                     /locus_tag="VGB76_05050"
     CDS             complement(1682..2098)
                     /locus_tag="VGB76_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0098303.1"
                     /translation="MSKELASLIPARGGRTDAGVYDDGRLRVEHDNYYVACDGRPLRC
                     PRVEFLLLSRLVGNAGRIVRAEDLWRYAWGNHKPYNAESLHVHIYRLRHKLAPFGIRI
                     ETMINVGYRLLSEESVPPRLPGNVEEQTPPRRSSLD"
     gene            complement(2235..6113)
                     /locus_tag="VGB76_05055"
     CDS             complement(2235..6113)
                     /locus_tag="VGB76_05055"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0098304.1"
                     /translation="MKLNICQLHRRLPMNLFRTQLLTGLRLTLLSLSICLMLTPSNYA
                     QSSPTGSLGGVVQDANGALLPGVNVNVKNAGTGQTRTATTNDEGRWTVPVLPVGMYEV
                     TIELTGFKRLVRQNVEVEASVPRTLEDRLEIGEITGEVVTITSGEAALVTSETATTSR
                     QVSSEELVAIPTSTRSFTQLLSSEAGVSAELSPVLTNGNGNQSPSVNGTRTTSTSLSF
                     NGIDATNITNNEGSLNDNIAPAPETLSEVKLQTSLYDASTGRSGGGNFQLITKSGSNE
                     FHGTGYIFAQNERFNANDFFYNKDGIDKPRARRLEGGFTLGGPIVKERFFFFGGYQRT
                     SASTGFVPTASSQTVLPAALRLINGARTADNIVAAFSALNPCVRNAANTPVSGFCLSP
                     SQISPIALNLLNITNPITGDFLVPAPRAGIATVFNDQAVAGTNVTLFGGNPLVRQRNV
                     FPAEFEQDQFNFKLDGQLSANNRLTGAFFFANFPGFDPFNAPNSQASPAGLVRNDRNR
                     TLAVSDIHIFSPTLINEVRFGYFSLNNSRALRDDFARITSESVGISNPALFFDNSVGT
                     QRLGQFIGRNNLANFSFGGPNDSFNRRKQQTYSLADNLTLIRGAHTLRFGGEFKRFAF
                     DTNLPEEQATEFEKFDNFTQFLAGRATEADTQFGITDKSFRFRDLSGYIADDWKFTRK
                     LTFNLGLRYEYFGTPEERNGQFGNFDPALLSNTENPLSGFVVPANARPTGFRALDDAL
                     AVTSRASNNSTINPDRNNFAPRFGFAYSPLDNNRMVIRGGYGIFFDRPSSAFINTVFS
                     NYPFLREIEVTAPSRQINLGTAFSQQNTQLGLNNFLPNRIQLVSTGGATTYVIRDNTG
                     VTRDANGALNPVDLATGQPSLGNIAETFEFRAVERNLRTPYVQQWNLGMQYELTKNLL
                     VEARYVGTKGTKLLQATALNRVYDLNDPNTPDYIYARFNQSYEAAQHGNPNALRGTLR
                     NAGTQRERGAGIAFGFRNSVTGNPLDLNLSDPNGSIIGFEARGALLGFNIPEALLLQS
                     NGNSIYHGAQFNLTQRLSRGLQFNASYTFSKSIDTSSADPGSTAGGGKPDVPNTGFIL
                     QNDSNDIGSNRALSDFDRTQRFSLSFVYDIPTFGSNSRLLRGFQLSGFAQVQSGAPFS
                     IFAAEPEAGNVSALTALRTGSGGLFRPGFGRPNLCGTLDQLREQSADPTEQYFNPAAL
                     CSSLGQYGNLGRNVLRGPRQKRFDLGLSKTTAITEGVGLELRWDVFNVFNNVNFANPN
                     NDLQDTTDFGRITNTVGGPRVMQFGAKLKF"
     gene            6366..6587
                     /locus_tag="VGB76_05060"
     CDS             6366..6587
                     /locus_tag="VGB76_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098305.1"
                     /translation="MSIANELSSDVATAVLARKNVETAQAKSELAGVVMKLHTTLRRL
                     TLDARRKKRRAQATPAEEPPRSNAAASGS"
     gene            complement(6680..7174)
                     /locus_tag="VGB76_05065"
     CDS             complement(6680..7174)
                     /locus_tag="VGB76_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0098306.1"
                     /translation="MKTIHLLRHAKSSWKDPGQSDFERPLNGRGREAAPLVGQFMLQK
                     KVRPNLILSSPAERARQTAALVIKAANLDAELRFDERIYEADAARLLEVVSQIDEAAG
                     VALLVGHNPGMEDLLTTLTGERHAMPTAALARIILDVEKWGKVREGAGRLDWLVRVKD
                     LIDT"
     gene            7395..8987
                     /locus_tag="VGB76_05070"
     CDS             7395..8987
                     /locus_tag="VGB76_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEY0098307.1"
                     /translation="MRFVICGECELSAEMKLAAIDIGSNSIKLAVVDAAASDSFAVLA
                     REKESVRLGHETLREGFLSPLAIERAADCVKRFRSIAEARGAEQVVAIATASVREASN
                     AGEFIDEVERATGVRVEVLSGVEEARLIGLAAASGCGVPDAPLINVDIGGGSTEISLM
                     RGNIPLALYSVKLGAVGLTERFLATDPPKQKELRALREEVRAALERPARELRGESWQR
                     ATGTSGTIIAVGEALRAQDARHRSQQGAQPTGAEIVFSRLSRFNERMAASSQTERRSV
                     PGISAQRSEIIIAGGHILEGAMRALGINHLRTCDWALREGVILDCLRELEAESRPPLP
                     VDADPRLRGVHAVGRRFGYEETHARQVTRFAETIFDKIAASASLTRHQRTLLSAAALL
                     HDVGYHIAHESHHKHALYLIKNSELTGFSEGEREVIANVARYHRGSTPKERHTDYSTL
                     NHADRETVRRLAAILRLADALDRSHDSRVLDLRCAHAGRTLSLELISENDCAREAAAA
                     EAKSDMFAEIFDCEVVIDHHRD"
     gene            9010..10212
                     /locus_tag="VGB76_05075"
     CDS             9010..10212
                     /locus_tag="VGB76_05075"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY0098308.1"
                     /translation="MAKAREIVGLNCEAEALSGIRLVLRTRFEEMYERRAEALNWEDI
                     EGVHDMRVASRRLRSAIKDFRDFLGRRSVPKRRLKKVADALGDVRDQDVAIEALEKLR
                     AEAGAEVSADVGAGIDQLIEERAALRGGARVKLAFIVAEKPLAELRENFLSRLESVGE
                     AEGGKDGRKSRRSASRASSFRQAGRRVISARIAELHDLSDSLHHPFDIEPLHELRIAA
                     KRLRYAMELFAPCWGGTLTSYAHEVSELQSSLGELRDCDTWIDDLGARLDRNRRRPAT
                     DLTFGTANQGQIRQAAVWLLDHFTKERGKHFRHALARWHEWELTGFFAQLESVLDGEQ
                     QPPASETDAAVVQEVPVVQEVSVVQTPEATIVQAVAPPPHVPDSEPQTDAPEPSVETS
                     PAHDNHVE"
     gene            10205..>11185
                     /locus_tag="VGB76_05080"
     CDS             10205..>11185
                     /locus_tag="VGB76_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098309.1"
                     /translation="MNKKVIILQLCCLLLLLAVACHNKATSTDEGRAAGPANSSPSTA
                     QTPAADSGDYEPPVHLANLEDRKIEESSGIVASRLNPGIYWTHNDSGDAPFIYAFDRS
                     GRSKGVWRVTGAEARDWEDIAAAPADGNGTSYLYIGDIGDNDRKGKVVTVYRIAEPGI
                     ESAGAEARGGAFQTEPAEAIRLEFPDGKHDAEALSVHPRTGDLYVITKTKRASCKVYK
                     ATAPFDTSTVVPLNFVGDLQLPGLQGGMLTGADISPDGQRVVLCDYFAAYELSLPRAD
                     ADFDSIWRQPLARIELGFRLQGEAICYRLDGKAILATSEQLPAPLIEVRRK"
BASE COUNT         2087 a         3150 c         3752 g         2196 t
ORIGIN      
        1 cgtgccgtag ataaaaggga gcgcgccgaa ttcgccctga atcgggtccc aagagcgccc
       61 ggtcaaaaac gagaagccga acgcttcgag cgagagccgc gcgttcgaga tcatcgagac
      121 gaacatcgcg ccgacgatga tgacgagcag cagcgcacag cagaagagca ggccgcgaaa
      181 gagcgcatcg cccgtgttgc ccgtgggcga cagagcgcgc cgccagttcc acgcgcgaaa
      241 gcgtgcgccg taggcggaag cgggcgtcgg caatgtcggc tgcaagttac tcataaattg
      301 aatgatgaaa gatgaacgcg gaagggtgaa tagaaaacag aattcagaat acaggagtcg
      361 gcatgaagac ccccgcaggt tttattctga cttctgttgt ctgaatcctg actcctgctt
      421 ctccttcatc gttcctcgtt cctacttcat cgtctagctc ctgagcggtt tgcctcccga
      481 tgtgatcgag tttattttgg cggcggcgcg ctcgacgatc tgcgggggca gcggcgcgta
      541 ctgcaaatct ttggcgaatc gttcgccgtc gtggatgccc caccagagaa agtctacgag
      601 cgccttgccc ttcgctgcgt ccttctggtc tttgtaggcg aggatgtagg tgtagctcga
      661 aatcgggtag gcgctcgcgc ccgccgtgtt ggtgatcgag acgcgcaggt cttcgggcgt
      721 ggctggcaaa ctctctgcgg cggcggcggt cacagcgtcc agactcggtt cgacgaaatt
      781 accggaactg tttttgataa gcgcgacggg cagtctgttc ggcgcggcgt aggcgagttc
      841 gacgtagccg atggtgttcg gcgtctgctt cacctgtccc gtcacgccct cgttgccctt
      901 gccgcccaag cccacaggcc agttcggcga agtgcccgag ccgaccttct ctttccattc
      961 ggggctgacc ttcgagagat agtccgtgaa gactgcggaa gtgccgctgc cgtccgaacg
     1021 gtggacgacg gtgaggtcca ccgccggaag ttggacgccc ggattgtcgg ccttgatgcg
     1081 cgcgtcgtcc caacgcttga tcttgccgag gaagatgtcc gcgatcactt cgggcgagaa
     1141 gcgcagcggc tctgcgatgc cctgcaagtt gtaggtgatg acgactgcgc cgagcacggt
     1201 cgggatgtgg agcagttcgc cgggcgcgct ccgcaagtcc tcgtccttca tcggtgcgtc
     1261 ggatgcgccg aagtctatgg tctgctcctt gatctgcttg atgcccccgc ccgacccgat
     1321 ggactggtaa tcgattctca cgttcgcgtt gagcttgccg tattcgctca gccatttttg
     1381 atagagcgga ttcgggaagg tcgcgcctgc gccttgcagg cggatcgtgc cgcccgccgc
     1441 gcctcccgtc ccgccccgct ggccgctgca cccgaaggcg agcgtggtga aagtgatgag
     1501 cagcagggcg agcgtcgcgc gtaaaagtct cggctgaggc agcatgaatg gtgtctcctg
     1561 tcggtgtgct ggtcttaaca tgacagccta gcgtagaccg gcgatgttaa atccgcctga
     1621 acagaatgat aaagatttgt taacactgaa gctgacgggg agcaccggaa ggcgcgctcc
     1681 gtcagtcgag tgaactgcga cggggcgggg tctgttcttc gacgtttcca ggcaggcggg
     1741 gcgggacaga ctcttccgag aggaggcggt agccgacgtt aatcatcgtc tcgatcctga
     1801 ttccgaacgg ggcgagcttg tggcggaggc gatagatgtg aacgtgcagg ctttcggcgt
     1861 tgtacggttt atggttgccc catgcgtaac gccagaggtc ttcggcgcgc acgatccgcc
     1921 cggcgttgcc gaccagacgc gagagcagca aaaactccac gcgcgggcac cgcaaaggcc
     1981 gcccgtcgca ggcgacgtaa tagttgtcgt gctcgacgcg caaacgcccg tcgtcataga
     2041 cacctgcgtc agtccgcccg cctcgcgccg gtatgagaga tgccaattct ttactcataa
     2101 gaaaaccaga aagcagtgac aagtgacggg tgacgagtga caagcaggaa gttaagacaa
     2161 gtttaaagag agcggtgctg atttaacttg tcacccgtca cttgtcactc gtcactcgtc
     2221 actcgtcact gcttttaaaa cttgagcttc gcgccgaact gcatgacgcg ggggccgccg
     2281 acggtgttgg taatccgtcc gaagtcggtc gtgtcttgca ggtcgttgtt cgggttggcg
     2341 aagttgacgt tgttgaaaac gttgaacacg tcccagcgca gttccagccc gacgccttcc
     2401 gtgatcgccg tcgtcttgga gaggccgagg tcgaagcgtt tctggcgggg tccgcgcagc
     2461 acgttgcgtc cgaggttgcc gtattggccg agcgaggagc agagcgcggc ggggttgaag
     2521 tattgctcgg tcgggtcggc tgattgctcg cgcaactggt cgagcgtgcc gcacagattg
     2581 ggacgaccga agccggggcg gaagagtccg cctgagcctg tgcgcagggc tgtgagcgcg
     2641 gagacgtttc cggcttccgg ttcggcggcg aagatgctga acggggcgcc cgattgcact
     2701 tgcgcgaagc ctgaaagctg gaagccgcgc aggagacgcg aattggaccc gaaggtggga
     2761 atgtcgtaga cgaagctgag actgaaacgc tgcgtgcggt cgaagtccga gagggcgcga
     2821 ttgctgccga tgtcgttcga gtcgttttgc aggatgaacc ccgtgttcgg cacgtcgggc
     2881 ttgccgccgc ccgccgtcga gccggggtct gccgaactcg tgtcgatgga cttcgagaaa
     2941 gtgtaggagg cgttgaactg taggccgcgc gagaggcgtt gcgtgagatt gaactgcgcg
     3001 ccgtgataga tggagttgcc gttcgattgc aggagcagtg cctccgggat gttgaagccg
     3061 agaagcgcgc cgcgcgcctc gaagccgatg atggagccgt tcgggtcgct caggttgagg
     3121 tcgagcggat tgccggtcac gctgtttctg aaaccgaaag cgattcccgc gccccgttct
     3181 ctttgcgtgc ctgcgttgcg aagcgtcccg cgcagtgcgt tcgggttgcc gtgctgcgcc
     3241 gcctcgtaag attggttgaa gcgcgcatag atgtagtcgg gcgtgttcgg gtcgttcagg
     3301 tcgtagacgc gattgagcgc ggtggcttgc agcagcttcg tgcccttcgt gccgacgtag
     3361 cgcgcctcga cgagtaggtt cttcgtcaac tcgtactgca tgccgagatt ccattgctgg
     3421 acgtaaggcg tgcggaggtt gcgttcgacg gcgcgaaact cgaacgtctc ggcgatgttg
     3481 ccgagcgagg gttgacccgt cgcgaggtca accgggttga gcgcgccgtt ggcgtcgcgc
     3541 gtgacgcccg tgttgtcgcg gatgacgtaa gtggtcgcgc cgcccgtaga gacgagttga
     3601 atgcggttcg ggaggaagtt gttaagaccg agttgcgtgt tctgctgcga gaaggccgtg
     3661 ccgaggttga tctggcgcga aggcgcggtg acttcgatct cgcgcaggaa agggtagttg
     3721 ctgaacaccg tgttgatgaa ggcggaggaa gggcggtcga agaagatgcc gtagccgccg
     3781 cgaatcacca tgcggttatt gtcgagcggc gagtaggcga agccgaagcg cggggcgaag
     3841 ttgttgcggt cggggttgat ggtggaatta ttggacgctc tggacgtgac ggcgagcgcg
     3901 tcgtcgagcg cgcgaaagcc ggtcgggcgt gcgttggcgg gcacgacaaa gccgctcaag
     3961 gggttctccg tgttcgacag caacgccggg tcgaagttgc cgaactgccc gtttcgctct
     4021 tcgggcgtgc cgaaatattc gtagcgcagg ccgagattga aggtgagctt gcgcgtgaac
     4081 ttccaatcgt ctgcgatgta gccggagagg tcgcggaagc gaaaactctt gtcggtgatg
     4141 ccgaactgcg tgtcggcttc cgtggcgcgc ccggcgagaa actgcgtgaa gttgtcgaac
     4201 ttctcaaact ccgtcgcctg ttcttctggc aggttcgtat cgaacgcgaa acgcttgaac
     4261 tcgccgccga agcgcaaggt gtgcgcgccg cgaatgagag tcaggttgtc tgcgaggctg
     4321 taggtctgtt gcttgcggcg gttgaaggaa tcgttcgggc cgccgaagga gaagttggcg
     4381 agattgtttc tgccgatgaa ctggccgagc cgttgtgtgc cgacgctgtt gtcgaagaag
     4441 agcgcgggat tcgagatgcc gaccgactcg ctcgtgatcc gtgcgaagtc gtcgcgcagc
     4501 gcgcgcgagt tgttgagaga gaagtagccg aagcggactt cgttgatgag cgtcgggctg
     4561 aagatgtgta tgtcggagac ggcgagcgtg cggttacggt cgttgcgcac cagaccggcg
     4621 ggcgaggctt gactgttcgg cgcattgaac gggtcgaagc cggggaagtt ggcgaagaag
     4681 aacgcgcccg tgagacggtt gttcgcactg agctggccgt cgagtttgaa gttgaactgg
     4741 tcttgctcga actccgccgg gaagacgttg cgctggcgca cgagcgggtt gccgccaaag
     4801 agcgtcacgt tcgttccggc gaccgcctga tcgttgaaga ctgtggcgat gcctgcgcgc
     4861 ggcgcgggga cgagaaagtc gcccgtgatc gggttcgtga tgttgagcag attgagggcg
     4921 atgggcgaga tttgcgaagg gctaaggcag aagccggaga cgggcgtgtt cgcagcgtta
     4981 cgcacgcagg ggttgagcgc gctaaaggct gcgacgatgt tgtccgccgt gcgtgcgccg
     5041 ttgatcaagc ggagcgcggc ggggagcacg gtctggctgc tcgccgtcgg gacgaagccc
     5101 gtgctcgcgc tcgtgcgctg gtagccgccg aagaagaaga atctctcttt gacgatggga
     5161 ccgccgagcg tgaacccgcc ttccagacga cgcgcgcggg gcttatcaat tccgtctttg
     5221 ttgtagaaga aatcgttggc attgaaacgc tcgttctgcg cgaagatgta gcccgtgccg
     5281 tgaaactcgt tcgagccgct cttggtgatg agttggaagt tgccgccgcc ggaacgaccc
     5341 gtcgaggcat cgtagaggct cgtctggagt tttacttcgg agagcgtttc gggcgcgggc
     5401 gcgatgttgt cgttcaagga accttcgttg ttggtgatgt tggtggcgtc gatgccgttg
     5461 aaggagagac tcgtgctcgt ggtgcgcgtg ccgttgaccg acggcgactg gttgccgttg
     5521 ccgttcgtga gcaccggcga aagctccgcg ctgacgcccg cttcggacga gagcagttga
     5581 gtgaaactgc gcgtcgaagt ggggatggcg acgagttctt cggaagaaac ttggcgggaa
     5641 gtggtggccg tttccgaggt cacgagcgcg gcctcgcccg aagtgatggt cacgacctct
     5701 cccgtgatct ctccgatttc gagcctgtct tcgagcgtgc gcgggacgga cgcttcaacc
     5761 tctacgttct ggcgcaccag acgcttgaag cccgtcaact cgatggtgac ttcatacatc
     5821 cccacgggca gcaccggaac tgtccaacga ccttcgtcgt tcgtcgtcgc ggtgcgcgtc
     5881 tgtcccgtgc ctgcgttctt cacgttgacg ttgactccgg gcagcagcgc gccgttcgcg
     5941 tcctgcacca cgccgcccaa actccccgtc gggctggact gtgcatagtt tgaaggggtg
     6001 agcattaggc agatgctcag agacaagagc gtgagccgga ggccggtcag caattgcgtg
     6061 cgaaagagat tcatcgggag cctcctgtgg agttggcaaa tgttgagctt catattttcg
     6121 attgagttca tgatgtcgtt gtgcacaaac ttagggctgc ccgcgcggga agtgcggcgc
     6181 ggcatattgt gtctcagtgt gatatgacga tcctgtgtgg cgagttggag gttaacgcgg
     6241 gcgggttaaa tcagagtgaa gtgtgcctta aatgtttgtt aactcgtgtc gggcttgcca
     6301 gaggcgtgag caggatgacg tttaaaagca ggattaccat cttcgaagcg gagtacgttc
     6361 cgctaatgtc aatcgccaat gaactgagca gcgatgtcgc gaccgccgtg ctggcgcgaa
     6421 agaatgtcga gactgcacaa gccaagagcg aactcgcggg ggtggtgatg aaacttcaca
     6481 cgacgctgcg ccgcttgacg ctcgatgccc gcaggaaaaa acggcgtgcg caggcaactc
     6541 ccgcagaaga gcctccacgt tcgaacgctg ccgcttccgg cagttaacgc gcacacgaca
     6601 gtctaaagtt gttgaggtct aaagttcctg cgatggcgac gcacgcttcc gcttcgcagg
     6661 gcgaagtgtg caagccgttt taagtgtcga tcaagtcctt cacgcgcacc agccagtcga
     6721 ggcgtcccgc gccttcacgc accttacccc acttctcgac atcgagaatg atgcgcgcca
     6781 gcgcagccgt cggcatagcg tgtctctctc ccgtcagcgt cgtcaataaa tcttccatgc
     6841 cggggttatg cccgacgagc agggcgacgc ctgccgcttc gtcgatttgc gagacgactt
     6901 cgagcaggcg cgcggcatcc gcttcgtaga tgcgctcgtc gaaacgcagt tcggcatcga
     6961 ggttcgccgc tttgatgacg agtgcggcgg tctggcgcgc acgctccgca ggtgaactga
     7021 gtattaagtt aggccgcacc ttcttctgca acatgaactg gccgacgagc ggcgcggcct
     7081 ctcgaccgcg accgttgagt gggcgttcga agtccgactg gccggggtct ttccaactcg
     7141 acttggcgtg gcgcaagagg tggatcgttt tcataatgtt atcgtatgtt tgaactacag
     7201 cgaattgcaa tcggaagaga cggcgggcac agagagggcg cagctaaaac tttagtctca
     7261 ggccgtaggg catcgctctc atttctatga ttcttggtcg gcctgtctgt tacaggcgcg
     7321 tgacaagtct tttgtataat cgcgattcgt taatgagata aatgagatag cggcgaacaa
     7381 ctcatctgtg cgggatgaga ttcgtaattt gcggcgagtg tgagttgagt gcagagatga
     7441 aactggcggc catagacatc ggttccaact cgatcaagct ggcggttgtg gatgcggcgg
     7501 cgagcgactc gtttgccgtt ctggcgcgcg agaaggagtc tgtgcgtctc ggtcacgaaa
     7561 cgctgcggga gggatttctt tcgccgctcg ccatcgagcg cgccgccgat tgcgttaaac
     7621 gattccgttc gattgcagag gcgcgcggag ccgagcaggt ggtggcaatc gccacggcgt
     7681 ctgtgcgcga agcgtcgaat gcgggagagt tcattgacga ggtggagcgt gcgacgggcg
     7741 ttcgcgtcga ggtgctgtcg ggcgtggagg aagcgcggct catcgggttg gcggcagcca
     7801 gcgggtgcgg cgtgccggat gcgccgctca tcaacgtgga catcggcggc ggctccactg
     7861 aaatctcgct catgcgcggc aacattccgc tcgcgctcta ctcggtgaag cttggcgcag
     7921 tcggcctgac ggagcgtttc cttgcgaccg acccgccgaa gcagaaagag ttgcgtgcgt
     7981 tgcgcgagga ggtgcgcgcc gcgctcgaac gccctgcgcg cgaactgcgc ggcgagagtt
     8041 ggcagcgtgc gaccggcact tccggcacga tcatcgccgt cggcgaggct ctgcgcgcgc
     8101 aggacgcgag gcacagatcg cagcagggcg cgcagcccac gggcgcggag atcgtcttca
     8161 gccgcttgag tcgcttcaac gagcgcatgg ccgcgtcgag ccagacggag cgacgcagtg
     8221 tgccgggcat cagcgcgcaa cgctcggaga tcatcatcgc gggcggccac atcctcgaag
     8281 gcgcgatgcg cgctctcggc atcaatcacc tgcgcacctg cgactgggct ttgcgcgagg
     8341 gcgtgatcct cgactgcctg cgcgaacttg aggccgagtc gcgtccgcct ctgcccgtag
     8401 acgccgatcc gcgcctgcgt ggcgtgcacg ccgtcgggcg acgcttcggc tacgaggaga
     8461 cgcacgcgcg ccaggtgacg cgcttcgccg agacgatctt cgacaagata gccgcttccg
     8521 cgtcgctgac tcgacaccag cgcacgctgt tgtccgccgc cgcgctgctg cacgacgtgg
     8581 gctaccacat cgcgcacgag tcgcaccaca agcacgcgct ctatctcatc aagaactcgg
     8641 aactgacggg cttctccgag ggcgagcgcg aagttatcgc caacgtcgcg cgttatcatc
     8701 gcggctcgac gccgaaagag cggcacaccg actactcgac gctcaaccat gccgaccgtg
     8761 aaaccgtgcg gagacttgcg gccatcctgc gtctggccga cgcgctcgac cgcagccacg
     8821 acagtcgtgt gctcgacctg cgctgcgcgc acgcgggacg aacgctcagt ctagagttga
     8881 tcagcgagaa cgattgcgcg agggaagcgg cggcggctga agctaaaagc gatatgttcg
     8941 cagagatctt cgactgcgaa gttgtgatcg accatcatag ggattaattt cggaagcgag
     9001 acattcaaca tggcgaaggc gagagagatc gtaggactca actgtgaggc ggaagccctg
     9061 agcggcattc gcctcgtgct gcgcacgcgg ttcgaagaga tgtacgaacg gcgcgcggag
     9121 gcgctcaatt gggaagatat cgagggcgta catgatatgc gcgtcgcgtc gcgcaggctg
     9181 cgtagcgcga tcaaggattt ccgcgacttc ctcggtcgca ggagcgtgcc caagcgtcgc
     9241 ctgaaaaaag tggccgatgc gctcggcgac gtgcgcgatc aggatgtcgc catcgaggcg
     9301 ctcgaaaagc tgcgcgcgga agcgggcgcg gaagtgagcg cagacgtggg cgcagggatt
     9361 gatcaactga tcgaggagcg ggctgcgctg cgcgggggcg cgcgggtgaa actggcgttt
     9421 atagttgccg aaaaaccgct cgcggagttg cgcgaaaatt ttctgtcgcg gctggaaagc
     9481 gtgggggaag ctgagggcgg aaaggatggc cggaagagtc gccgcagcgc gtcgagggcg
     9541 tcgagtttcc ggcaggccgg gcgtcgtgtg atctccgcgc gcatcgcaga gttgcatgat
     9601 ttgagcgaca gtctccacca cccgttcgac atcgagccgc tccacgaact gcgcatcgcg
     9661 gccaagcggc tgcgttacgc gatggaactg ttcgcgcctt gctggggcgg gacgctcacc
     9721 tcctacgcgc atgaagtgtc cgagttgcaa tcttcgctcg gcgagttgcg cgactgcgat
     9781 acgtggatcg acgatctcgg cgcgcggctc gaccggaatc gccgtcggcc tgcaaccgac
     9841 ctgacgtttg ggacggccaa tcagggacag atcaggcagg ccgccgtctg gctcctcgac
     9901 cacttcacga aggagcgcgg caagcatttt cgacacgcgc tcgcacgctg gcacgagtgg
     9961 gagttgacag gcttcttcgc gcaactggaa tccgtcctcg acggagagca gcagccgccc
    10021 gcgagcgaga ctgatgcggc ggtcgttcag gaagtgcccg tcgttcagga agtgtctgtc
    10081 gtccagacgc cggaagcgac gattgttcag gcggtcgccc cgccgccgca cgttcccgat
    10141 tccgagccgc aaacggatgc gcccgagccg tcggtcgaaa cttctcctgc gcacgacaat
    10201 cacgttgaat aagaaagtta taattcttca actttgctgc ctgctgctat tgctcgccgt
    10261 cgcatgccat aacaaggcga cttcgacgga cgaaggcagg gctgccggtc ctgcgaactc
    10321 ttcgccctcc acggcacaga cgcccgccgc cgactccggc gattacgagc cgcccgtgca
    10381 tctcgcaaac ctcgaagacc ggaagatcga ggagagcagc ggcatcgtcg cctcgcgtct
    10441 caatcccggc atctactgga cgcataacga ttcgggcgac gctcctttca tttatgcgtt
    10501 cgaccgcagc gggcgttcga agggcgtctg gcgcgtgacg ggcgcagagg cgcgcgactg
    10561 ggaagacatc gccgccgcac ccgccgacgg caacggaacg tcctatcttt atatcggcga
    10621 catcggcgac aacgaccgga agggtaaggt ggtcaccgtc tatcgcatcg ctgagccggg
    10681 catcgagtct gccggagcag aagcgcgcgg cggcgcgttt cagaccgaac ccgcagaggc
    10741 tatccggctg gagtttccgg acggcaagca cgacgccgaa gctctctcgg ttcaccctcg
    10801 aaccggcgac ctgtacgtca tcaccaagac taaacgcgcc tcctgtaaag tgtataaagc
    10861 cacggctccg ttcgacactt cgacggttgt tccgctcaac ttcgtaggcg acctgcaact
    10921 ccccggccta cagggcggga tgctcaccgg ggcggatatt tcgccggacg gccagcgcgt
    10981 cgtgctatgc gattatttcg ccgcgtatga actctccctg ccgcgtgcgg atgccgattt
    11041 cgatagtatc tggcggcaac cgctcgcacg catcgagttg ggctttcgct tgcagggcga
    11101 agccatctgt taccggctcg acggaaaagc catcctcgca acgagcgaac aactccccgc
    11161 tcctctgatc gaagttcgac gcaag
//
LOCUS       DASWNS010000150         4338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_343454, whole genome shotgun sequence.
ACCESSION   DASWNS010000150 DASWNS010000000
VERSION     DASWNS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343454"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..412
                     /locus_tag="VGB76_05085"
     CDS             41..412
                     /locus_tag="VGB76_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0098310.1"
                     /translation="MAKSIERYLRFMPVTKPGRLCERHWSPPADVYRTRDGWLVKVDL
                     AGVSPDELEITIAGPMLVIEGTRRDTSCSETISYHQVEISYSRFEKSLRFPCPIEGAR
                     IESDYQDGLLLLRLRSPEECE"
     gene            525..2999
                     /gene="lon"
                     /locus_tag="VGB76_05090"
     CDS             525..2999
                     /gene="lon"
                     /locus_tag="VGB76_05090"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0098311.1"
                     /translation="MAENSTNTPDAAASATQTPAAQAFEIAALPLQNTTLFPGTVVPL
                     AAGRPRSIAAVEAALGQPEKLLACFSVRPGRATEAEALPEDIYPVGTLVMVKRMFRGE
                     DALQIIVQGTERVRLSEWTQTDPFLRARVEVLPPLRTVDQQAVEALQRNVRVLIERAL
                     AILPQVPPEVRAAVMNATDPTQIAYFLASVLNLGVEHEQAMLEADTTDELLRLAHARL
                     AREVEIMQLTSKIATEAQTEMDKAQRDYVLRQQMKAIQKELGDEDGEDAEAALMRERL
                     GEADLPDEVRVEAERELKRMERLPAAAPDYHVIRTYLEYVLELPWRKGSEDRLDLAEA
                     RRVLDEDHYGLEDIKERILEFLAVVKLRPDSKSPILCFVGPPGVGKTSLGRSIARAMG
                     RAFERLSLGGMRDEAELRGHRRTYIGAMPGRIIQSLRRAQVNNPVLMLDEIDKLGADF
                     RGDPASALLEILDPQQNNTFRDHYLDLPFDLSKVFFIATANQLAPIPPPLRDRMEIIA
                     LAGYSDQEKLHIALQYLVPRQVGENGLRPEQFSLSNEAIMMIATRYTREAGVRQLERA
                     IGSVARKAALRIAQGEATEVRVEAGDIKEFLGAPRFYPEEARKELPAGVATGMAWTEM
                     GGQILFIEATLLPGGSGLTITGQLGEVMQESARAARSYLWSHASEFGIPAEMFKSYGV
                     HLHVPAGAIPKDGPSAGVAITAALASLMTGRRVSHNTSMTGEITLSGLVFPVGGIKEK
                     VLAAHRAGIRRIILPSRNEADLEDIPAEVRQELEIKLVSRISEVLDAALEKLVANPPP
                     PVTTDAGARNQATGQAQPEALRVLEP"
     gene            3102..3419
                     /locus_tag="VGB76_05095"
     CDS             3102..3419
                     /locus_tag="VGB76_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098312.1"
                     /translation="MVNKKAQSKAGIPANRRGQQMGDERSNSPVKQEEAEENTPALRG
                     KRKDANAFFADDSAQHVGSNAARPRSNTPSLPALPTNVKKGESGGERVFKQRQAKKSG
                     RGK"
     gene            complement(3426..4118)
                     /locus_tag="VGB76_05100"
     CDS             complement(3426..4118)
                     /locus_tag="VGB76_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098313.1"
                     /translation="MYRRIIHAIAALLSAYLIGGTVSAVVRGMISRLEYAEDYTRWEA
                     DVLGALRDTGVDPGMWVLGSWLAVWLWCELVVYRRGAGGKRSLLLLHLRYAPLATCAF
                     YLIWLPPSEQLMIEHSRWQITRYIYSDAPPEVSPTFDLYNDYKGWCGNGYAAHEYWLY
                     GETAAARFESDDPRVRARAFQATRLVYDWVNLPPNGVFDEVLRRARVDPDPLVRRLAA
                     EHCSKQYVNCPP"
BASE COUNT          889 a         1382 c         1315 g          752 t
ORIGIN      
        1 cactcatcac tcatcactca ttactcatca ctgtgaaaaa atggctaaat caattgagcg
       61 ttatttgcgt ttcatgccgg tgacgaagcc ggggcgtctg tgcgagcggc actggtcgcc
      121 cccggctgat gtctatcgca cgcgcgacgg ctggctcgtc aaagttgatc tcgcgggcgt
      181 cagcccggac gagttggaga tcacgattgc ggggccgatg ctcgtcatcg aaggcacgcg
      241 ccgcgacact tcttgcagcg agacgatctc gtatcatcaa gtggaaatct cctacagccg
      301 tttcgagaag tctctgcgct tcccttgccc gattgaaggc gcgcgcatcg aaagcgatta
      361 tcaggacggg ttgttgctgc tccgcctgcg cagcccggag gaatgcgaat aggaaattgt
      421 ccgtcgtcag tagtcagttg tccgttgttt gtgccggacg tgcaagtggc taaagaactg
      481 tttgaacaac tgacaacggg cgactgacga cggataaaat tatcatggct gaaaattcaa
      541 cgaacacacc cgacgccgcc gcatccgcga cgcagacgcc tgccgcacag gccttcgaga
      601 tcgcggcgct ccctttgcag aatacgactc tgtttcccgg cacggtcgtg ccgctcgcgg
      661 cgggtcgccc gcgttcgatt gcggcggtcg aggccgcgct cgggcagccg gagaagttgc
      721 tggcctgctt cagcgtcagg ccgggcagag ccacggaggc cgaagcgttg cccgaagata
      781 tatatccggt gggcacgctc gtgatggtca agcggatgtt tcgcggcgag gatgctttgc
      841 agatcatcgt gcagggcacg gagcgcgtgc gcctctccga atggacgcag accgatcctt
      901 ttttgcgcgc gcgggtcgaa gtgttgccgc cgctgcgcac ggttgaccag caggcggtcg
      961 aagcgttgca gcgcaacgtg cgcgtgctca tcgagcgcgc tctggcgatc ctgccgcagg
     1021 tgccgcccga agtgcgcgcc gccgtgatga acgcaaccga ccccacgcag atcgcttact
     1081 ttctcgcctc cgtgctcaac ctcggcgtcg aacacgaaca ggccatgctc gaagccgaca
     1141 cgaccgacga gttgctccgt ctcgcacatg cgcggctcgc gcgcgaagta gagatcatgc
     1201 agttgacttc caagatcgcc accgaagcgc agacggagat ggacaaggcg cagcgcgatt
     1261 acgtcctgcg ccagcagatg aaagcgatcc agaaggaact cggcgacgag gacggcgaag
     1321 acgccgaggc cgccttgatg cgcgagcgtc tcggcgaagc cgacctgccc gacgaagtgc
     1381 gcgtcgaagc cgaacgcgaa ctcaagcgca tggaacgcct gcccgccgcc gcgcccgatt
     1441 atcacgtcat ccgcacctat ctggaatacg tgctcgaact cccctggcgc aaaggttccg
     1501 aagaccgtct cgatctggcg gaagcgcgcc gcgtgctcga cgaagaccac tacgggctgg
     1561 aagacatcaa ggaacgcatc ctcgaatttc tcgccgtcgt caaactgcgc cccgactcga
     1621 agagtccgat cctctgcttc gtcggacctc cgggcgtcgg caaaacttcc ttagggcgtt
     1681 cgattgcgcg tgcgatgggg cgcgcgttcg aacgcctctc cttaggcggc atgcgcgacg
     1741 aagcggaact gcgcgggcat cgtcgcacct acatcggcgc gatgcccggt cgcatcattc
     1801 aatctctgcg ccgcgcgcag gtcaacaatc ccgtcctgat gctcgacgag attgacaaac
     1861 tcggcgcgga ctttcgcggc gacccggcga gcgcgctctt agagattctc gacccgcagc
     1921 agaacaacac tttccgcgat cactatctcg atctgccctt cgatctctcg aaggtcttct
     1981 tcatcgcgac ggcgaaccaa ctcgcgccca tcccgccccc gctgcgcgac cgcatggaga
     2041 tcatcgcgct cgcgggctac agcgatcagg agaaactgca catcgccttg cagtatctcg
     2101 tgccgcgaca ggtcggcgaa aacggcctgc gccctgaaca attttccctc tcgaacgaag
     2161 cgatcatgat gatcgccacg cgttacacgc gcgaggcagg cgtgcgtcaa ctcgaacgcg
     2221 ccatcggttc ggtcgcgcgc aaagccgcgc tgcgtatcgc gcagggcgag gcgacggagg
     2281 ttcgcgtcga ggccggagac atcaaggagt ttttgggcgc gccgcgtttc tacccggaag
     2341 aggcacgcaa ggagttgccc gcaggcgtgg cgacgggcat ggcttggacg gagatgggcg
     2401 ggcagatact cttcatcgaa gcgacgcttc tgcccggcgg ttcggggctg acgatcacgg
     2461 ggcaactcgg cgaagtgatg caggaatcgg cacgcgccgc acgctcttat ctctggtcgc
     2521 acgcctcgga gttcggcatc ccggcggaga tgttcaaaag ttacggcgtg catctgcacg
     2581 tccctgcggg cgcgattccg aaagacggcc cttcggcggg cgtcgccatc acggctgcgc
     2641 tcgcgtctct gatgaccggg cgacgcgtca gccacaacac ttcgatgacg ggcgagatca
     2701 cgctttcggg gctcgtcttc cccgtcggcg gcatcaagga gaaagtgctc gccgcgcatc
     2761 gcgcaggcat caggcgcatc atcctgccct cgcgcaacga ggccgacctc gaagacattc
     2821 cggcagaggt gcgccaggag ttggagatca aactcgtctc gcgcatcagc gaagtgctgg
     2881 acgccgcgct cgaaaaactg gtcgccaacc cgcccccacc cgtcacgacg gatgcgggcg
     2941 cgcgtaatca agcgaccggg caggcgcaac ccgaagcgtt gcgcgtcctc gaaccttaag
     3001 cgcagaggaa gtaatcgagt ttcaagtccc gggtcgagag gggctttgag acttgggact
     3061 tgaaacttta gttattaaag attcagacag ggagcgacaa cgtggtcaac aaaaaggcgc
     3121 agagcaaagc gggcatcccc gccaaccggc gcgggcaaca gatgggcgac gagcgcagca
     3181 acagcccggt caaacaggaa gaggcagagg agaacacgcc cgccctgcgc ggcaaacgca
     3241 aagacgcgaa cgccttcttc gcagacgact ccgcgcaaca cgtgggcagc aacgccgccc
     3301 gcccgcgttc caacacgcct tcgcttcccg ccctccccac caacgtcaag aagggcgaat
     3361 ccggcggcga gcgcgtcttc aaacagaggc aggcgaagaa gagcggtcgc ggcaaataag
     3421 agattctacg gcggacagtt tacgtactgc ttcgaacagt gttctgcggc cagtctcctg
     3481 acgagcgggt ctgggtccac tctcgcccgc cgcaagacct cgtcgaatac gccgttcggc
     3541 ggcaggttga cccaatcata aacaagccgg gtcgcttgaa aggcgcgcgc acgcacccgt
     3601 ggatcgtcgc tctcgaaccg ggcggctgcc gtctccccgt acagccagta ttcgtgcgcc
     3661 gcgtatccgt taccgcacca gcccttgtaa tcgttataga gatcgaacgt ggggctgacc
     3721 tccggcggcg catcgctgta gatgtagcgt gtgatctgcc agcgtgaatg ctcgatcatg
     3781 agttgttccg aggggggcaa ccagatcagg tagaaggcgc aggtcgccag aggcgcgtag
     3841 cgcaggtgca acaacagcaa cgaacgcttg ccgcccgccc cgcgtctgta gacgaccaac
     3901 tcacaccaga gccacacggc cagccagctg cccaagaccc acatacccgg atcaacgccc
     3961 gtgtctctga gcgcgcccag cacgtctgcc tcccagcgcg tgtaatcctc cgcatactca
     4021 agccttgaga tcatccctct gacaactgcg ctcaccgtgc ctccgatgag ataggctgag
     4081 agcaacgcgg ctatcgcatg aatgattcgt cggtacatgg cttcctcccg cccgtcgtgc
     4141 cgttgaaacg caagcggttt gatagacacc gcgcgcgccc cttctgttcc gccggaacca
     4201 attgagggac acgctcagaa tttgtgagca gattggagat cggaatactg ctaaggggcg
     4261 cgagcgagtt cctgcgcaag ccgcacgacc tcttccgctt gttggacatc gcacgcaggc
     4321 ttgttgagac aaaacgat
//
LOCUS       DASWNS010000151         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_110725, whole genome shotgun sequence.
ACCESSION   DASWNS010000151 DASWNS010000000
VERSION     DASWNS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110725"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..678
                     /locus_tag="VGB76_05105"
                     /pseudo
     CDS             1..678
                     /locus_tag="VGB76_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
     gene            1304..>2361
                     /locus_tag="VGB76_05110"
     CDS             1304..>2361
                     /locus_tag="VGB76_05110"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY0098314.1"
                     /translation="MLQRSLLTILILLPVAGALVAVLYGMAARRSETAAKWIALIFST
                     ATFALSLLLLTGGGYADSAAFRFEQNLPWIGAIGARYHVGVDGISLWLVILTTLLVPI
                     SIVSSWHAVEKRPLAFYSFILLLESAMLGVFVSLDLLVFYLFFEASLVPMFFLIGVWG
                     GERRIYAAAKFFIFTAVGSLLMLVGIIALYYIHKNQAGFGTFDYSTLLSSLRSGQMTL
                     GTDAQFWLFLAFAIAFAIKVPLFPFHTWLPDAHTEAPTAGSVILAGVLLKMGTYGLMR
                     FNLALFPDAARRFAPVLITLAVIGIIYGALVAMVQPDVKRLVAYSSVSHMGFVVLGIF
                     SFTEQGMQGALYQMLNHGV"
BASE COUNT          393 a          778 c          717 g          473 t
ORIGIN      
        1 ctgctgttcc tgggcgcggg ctcggtgatc cacggcatgc accacgaaca ggacatgcga
       61 cggatgggcg gcttgaagaa gtacatgccg atcaccttcg cgacgatgct cacgggctgg
      121 ctcgccatca gcggcattcc catcttcgcg ggcttcttct cgaaagacga aatcttgtgg
      181 aagacatgga gcacgggcgc aaccgctatc cctgtgtggg cgagcaaaat gctctgggtc
      241 gtcggcgcgt tgacggctct gctcacggca atctacatga cgcgcctgat ggtcttgacc
      301 ttctggggca gcgagcgttt ccgcgaagcg caggccggag ggcaggccga cgaggcgcac
      361 gcacacgcct atgacgaagg cgacaaggcg cacgacgcct cacgcttctc cgcaggcgac
      421 cgcccgcaac atgaaccggg cgcgcacgtc ggcgacgcgc acgcacccgc gcacgctccc
      481 gcaaacgcgc acgatgacga cgaagacgag cacgacgcgc accaccatca aggacccttc
      541 acgccgcacg agtcgccctg gacgatgacc gtcccgctcg tcgtgctggc gatactctcg
      601 accttcggcg gcctgctcgg cgtgccttac gcgctcagcg gcggcgcggt gaacaactat
      661 ttcgaacacg cgcttgagcc ggtcgtggaa cgcgcgccgc aacgcggggg cggtcacgcg
      721 gaggtcggcg tcggcacacc gtcagcgcat ggcgcgacgc ctgcgaccga ttcgcacggc
      781 tcgcccgccc cgtccggatc gcatgctccg agccagtcgc acggaaacga gggagcgttg
      841 ccgccttcgg tcggagaagg acagcacggc ggcgcgcctg ccgccgaaca cacgcacgat
      901 cccaatgaag tcttctggga gagagttttc agcggcatct ccatagccat cgcgctcgca
      961 ggcatcttcg gcggctggat gtacttcaag aagaacccgt tgaagcgtat gccgcgcctg
     1021 ttggaaaaca agtactacgt ggacgaagcc tacgatgccg cgatcatcaa tcccatcaag
     1081 gttggttcgc gcgaggggct gtggaagatt ttcgatgtca gggtggttga cggtctggtc
     1141 aacgggctgg cgcgcgcgac gagcggcgtg ggcgacctcg cgcgccggat gcaggcaggg
     1201 ttcgtgcgcg gctatgcggc catcatcctg ctcggcgcgc tcgtcgtgat cggctacttc
     1261 gtcttcaact tcagccgcgt gctgtcgctt cattaaacaa cttatgctcc agcgttcgct
     1321 cttaaccatc ttgatcctgc tgcccgtcgc gggcgcgctc gtcgccgtcc tttacggcat
     1381 ggccgcgcgc cgcagtgaga cggcggccaa atggatcgcg ctcatctttt cgacggcgac
     1441 cttcgcgttg tcgctcctgc tcctgacggg cggcggctac gcagactcgg cggcgttccg
     1501 tttcgaacag aacctgccct ggatcggggc tatcggcgcg cgttatcacg tcggcgtgga
     1561 cggcatcagc ctgtggctcg tcattctcac cacgctgctc gtgccgatct ccatcgtctc
     1621 aagctggcac gcggtggaga agcgaccgct cgccttctac tcgttcatcc tgctgcttga
     1681 gagcgcgatg ctcggcgttt tcgtctcgct cgacctgctc gtcttttatc tcttcttcga
     1741 ggcgtcgctc gtgccgatgt tttttctcat cggcgtgtgg ggcggcgagc ggcgaattta
     1801 cgcggcggcg aagtttttca tcttcacggc tgtcggcagc ctcctgatgc tcgtcggcat
     1861 catcgcgctc tactacatac acaagaatca ggcgggcttc gggacgttcg actactcgac
     1921 cctgctctcg tcgcttcgca gcggccagat gacgctcggc acagacgcgc agttctggct
     1981 cttcctcgcc ttcgccatcg ccttcgccat caaagtgccg ctcttccctt ttcacacgtg
     2041 gctgccagac gcgcacaccg aagcgccaac cgctggctcc gtgatcctcg cgggcgtgct
     2101 cctgaagatg ggaacttacg gcctgatgcg ttttaatctc gcgctcttcc ccgacgccgc
     2161 gcgcagattc gcacccgtcc tcatcacact tgcagtgatc ggtatcatct atggcgcgct
     2221 cgtcgcgatg gtgcagccgg atgtcaaaag actcgtggct tattcgtccg tgagccacat
     2281 gggattcgtc gtgctcggca tcttttcgtt caccgaacag ggcatgcagg gcgcgctcta
     2341 ccagatgctc aatcacggcg t
//
LOCUS       DASWNS010000152         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_422177, whole genome shotgun sequence.
ACCESSION   DASWNS010000152 DASWNS010000000
VERSION     DASWNS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422177"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /locus_tag="VGB76_05115"
     CDS             <1..1628
                     /locus_tag="VGB76_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY0098315.1"
                     /translation="LERGREFFENNYLKPNRRFILGTLAYQTPLARWTFEYWEGELIP
                     TEQIKLTGELINRTFFERVAFKPNSIRQDEASAAIAGLERVSQSEIAAEQSYQPLNVA
                     RGLGRIHIIKKLDEHVEIGFNEILVLDEVPVTLPPVAGIITSKPSTPLSHINLLAKSW
                     GVPNAYVKNAAELLKEYDGWWVSFETKQGKYEIKRADNNALNEYQQRLKERRDIMTPR
                     FDLSEKRLADLRQQRANMADAYGAKSANLGEVANARLRGIVVPNGFTIPIYYYDEFIK
                     ANELEKPIYEMLDDQKFVHDPAYRRERLAEMRARIQGAKFQDDLRKAVLRRVHAEYAG
                     KGLFVRSSTNAEDLPKFNGAGLYSTVPNVREDEKLIEAIKTVWASVWNFEAYEARERA
                     AIDHMKVYPAVLIQEGINADSAGVMITTDPYDETNPAGIYISAKRGLGIKVVEGKRIA
                     EQIIFSPRSQAVRVLTRSDEESLLTFDDQGGVKEIPISGTRAVLTDATVRRLARAAAS
                     IKRVFRGREQDIEWVFYGGQVYIVQSRPYIAGS"
     gene            1815..2300
                     /locus_tag="VGB76_05120"
     CDS             1815..2300
                     /locus_tag="VGB76_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098316.1"
                     /translation="MRTFVGLLSLVSALLLCGACGESAPAGANENGAANSSAASSGAN
                     SSGASNHNGGGGGQAYDTSKMDAEIAQLEQQAEKNPDDDTTHNALADAYVRRGRALHE
                     AHRLPDALHDYQNALRFSPDHEEANLRIAQITQEIGAEPHAEDGKPVAVPAKPGANEN
                     Q"
     gene            2427..3221
                     /locus_tag="VGB76_05125"
     CDS             2427..3221
                     /locus_tag="VGB76_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0098317.1"
                     /translation="MTESVFVEGLLAGRVAFVTGGGTGITGGVARALAKAGARVALVS
                     RKLEHLEPAAEVIRADGGEALAWACDVRQAEEVERAVAATVERFGGLDIVVNGAAGNF
                     LCPAEELSPNGFGTVVDIDLKGTFNVCRAAFAELKKRGGSILNISATLHYLGTPLQLH
                     VSAAKAGVDALTQNLAVAWGRYGIRTNAIAPGPIEDTEGMKRLLPPALKEKLQRRIPV
                     GRFGRIKDIEHAAVFLCSDAASFINGAIIVVDGGQWLAASSLLGSD"
BASE COUNT          724 a          927 c         1056 g          637 t
ORIGIN      
        1 cgctcgaacg cgggcgcgag tttttcgaga acaactatct gaagcccaac cgccgcttca
       61 tactcggcac gctcgcctac cagacgccgc tcgcgcgttg gactttcgaa tattgggaag
      121 gtgagttgat ccccaccgag cagattaaac tcacgggcga actcatcaat cgcaccttct
      181 tcgagcgcgt cgccttcaag cccaactcca tcaggcagga cgaagcctcc gccgccatcg
      241 ccggactcga acgcgtctcg cagagcgaga tcgcagccga gcagtcctat caacctttga
      301 acgtggcgcg cgggctcgga cgcatccaca tcatcaaaaa gctggacgag cacgtcgaga
      361 tcggcttcaa cgaaattctg gtgctggacg aagtgcccgt cactttgccg cccgtcgccg
      421 ggatcatcac gagcaagcct tcgactccgc tctcgcatat caatctgctc gccaagtcgt
      481 ggggcgtgcc gaacgcgtat gtcaaaaacg ccgccgagtt gctcaaagaa tatgacgggt
      541 ggtgggtttc cttcgagacg aaacagggca agtatgagat caagcgcgcc gacaacaacg
      601 cgctgaatga ataccagcag cggctcaaag agcggcgcga catcatgacg ccgcgcttcg
      661 acctctctga aaaacgtctg gccgacctgc gacagcaacg cgcgaatatg gccgacgctt
      721 acggagcgaa gtcggccaac ctcggtgagg tcgcaaacgc gcgcttgcgc ggcatcgtcg
      781 tgccgaacgg gttcaccatt cccatctatt attacgacga gttcatcaaa gcgaacgaac
      841 tcgaaaagcc gatctacgag atgctggacg atcaaaagtt cgtacacgat ccggcttatc
      901 ggcgcgagcg tctcgcggag atgcgtgcgc gcatacaggg ggcgaagttt caggatgatc
      961 tgcgcaaagc ggtcttgcgg cgcgtgcatg cggagtatgc ggggaagggc ttattcgtgc
     1021 gcagttcgac gaacgcggaa gacctgccga agttcaacgg cgcgggactc tattcgaccg
     1081 tgccgaacgt gcgcgaagat gaaaagttga tcgaggcgat caagaccgtc tgggcttccg
     1141 tctggaactt tgaagcctac gaggcgcgcg aacgcgccgc gattgatcac atgaaagtct
     1201 acccggcggt gttgattcag gagggcatca acgcagacag cgcgggcgtc atgatcacga
     1261 ccgaccctta cgacgagacg aacccggcgg gcatctacat cagtgcgaaa cgcggcctcg
     1321 gcatcaaggt ggtcgaaggc aaaaggatcg cggagcagat catcttcagc ccgcgctcgc
     1381 aggccgtgcg ggtgctgacg cgttcggatg aagagagcct gctgacgttt gacgatcaag
     1441 ggggcgttaa agaaattccg attagcggca cgcgtgcggt cttgacggac gcgaccgtgc
     1501 gcaggctggc gcgcgccgcc gcttcgatca agcgcgtctt tcgcgggcgc gagcaggaca
     1561 tcgaatgggt cttctacggc ggccaggtct acatcgtgca gtctcgaccc tacattgcgg
     1621 gaagctgaaa aagagagtga caggtgacga gtgacaagtg acgagagaag aaagtaaatc
     1681 gtgaatcgta aatcgtgaat agacggaagc agtagtcttt ctattcacga gtgacgattc
     1741 acgattcacg gcttctgttt taacttgtca ctcgccgctt gccacgcgtc gctgcactta
     1801 aaatggtgaa tctgatgcga actttcgttg gtctgttgag cttggtgtcg gcgttgttgc
     1861 tgtgcggcgc atgcggcgag agcgcgcctg cgggtgcgaa tgagaatggc gcggcaaatt
     1921 cgtctgcggc ttcgagcggg gcgaatagca gtggcgcgtc gaatcataac ggcggcgggg
     1981 gcgggcaggc ctacgacacg tcgaaaatgg atgcggagat cgcgcagctt gaacagcagg
     2041 cggagaagaa tcccgacgac gatacgacgc acaacgctct ggctgacgcc tacgtgcggc
     2101 gcggtcgtgc gctccatgaa gcgcaccgcc tgcccgatgc cttgcatgat tatcagaacg
     2161 cgctgcgttt cagtcccgac catgaagagg cgaatttgcg catcgcgcag atcacacagg
     2221 agatcggagc cgagccgcac gccgaagacg gcaagcccgt cgccgttccg gccaagccgg
     2281 gagcgaatga gaatcaatga tgaaaagcag gagccagcat tcagaatcca gccgtcagaa
     2341 taaaaggcgc gcgtctcttt cttctgactc gtgtgttctg aattctgtgt tctgtcttct
     2401 ccttcatcgt ttttgaaagg ttgcgtatga cagagagcgt gttcgtcgaa gggttgctgg
     2461 ctggtcgggt cgcgtttgtg acgggcggcg ggacggggat cacgggcggc gtggcgcgtg
     2521 cgctggcgaa ggcgggggcg cgtgtcgcgc tcgtcagccg gaagttggag catctggaac
     2581 cggcggcgga agtgattcgc gcggacggcg gggaagcgtt ggcgtgggca tgcgatgtcc
     2641 gacaggcgga agaggttgag cgcgccgtcg ccgcgacggt cgaacgcttc ggcgggttgg
     2701 atatcgtcgt caacggagcg gcgggaaatt ttctctgtcc ggcggaggag ctttcgccca
     2761 acggtttcgg cacggtggtt gacattgatc tgaaagggac gttcaacgtc tgccgcgcgg
     2821 cgtttgccga actgaagaag cgcggcggct cgatcctgaa catcagcgcg acgctgcatt
     2881 atctgggcac gcctttgcaa ctccacgtct cggcggcgaa ggccggagtg gatgcgctca
     2941 cgcaaaatct agcggtcgcg tggggacgct acggcatccg caccaacgcc atcgcgccgg
     3001 gtccaatcga ggacacggag ggcatgaagc gtctgctccc gcccgcgctc aaagagaagt
     3061 tgcaacgccg cattccggtc ggtcgcttcg gacgaatcaa ggacatcgag cacgcggcgg
     3121 tcttcctctg ttccgacgcc gcaagtttta tcaacggcgc gatcatcgtc gttgacggcg
     3181 gccagtggct cgccgccagc agtctcttag gcagcgatta gatagattta agacagcaaa
     3241 tcgtgaatcg tcaatcgtga atagagaaag cctgctttag tctattgacg attgacgatt
     3301 cacgattcac gctctttatg cttgaacttc agcccgcgac gttt
//
LOCUS       DASWNS010000153         6475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_135879, whole genome shotgun sequence.
ACCESSION   DASWNS010000153 DASWNS010000000
VERSION     DASWNS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135879"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..514
                     /locus_tag="VGB76_05130"
     CDS             113..514
                     /locus_tag="VGB76_05130"
                     /inference="COORDINATES: protein motif:HMM:NF025183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HEY0098318.1"
                     /translation="MRDDESVNVSQRNLKEVFDALAGSPFRRRFRLRGKELEYLRRKG
                     LIVVMEHAADFIEKRLAPASPARDGKQTPMGHHPVFIAQHATATCCRGCLQKWHGIAK
                     GRALEATERAYILEVIRRWLAREEELNEGTT"
     gene            498..935
                     /locus_tag="VGB76_05135"
     CDS             498..935
                     /locus_tag="VGB76_05135"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0098319.1"
                     /translation="MKELLETYPLVIEIPVAWGEMDSLRHVNNIVYFRYFESARMAYF
                     QKLDIWNYMNETGIGPILASTQCRFKIPLTYPDIVSVGTKISEIEADRFNMKYVVVSH
                     AHAKVAAEGEGLIVSYDYRAQKKAALPDVIKERIRILEDSARR"
     gene            944..1963
                     /locus_tag="VGB76_05140"
     CDS             944..1963
                     /locus_tag="VGB76_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY0098320.1"
                     /translation="MNDKTNRKILLASRPAGMPTLDNFEIADAGMPQPQAGEVLIRTL
                     YLSVDPYMRGRMSERKSYIPPFALNEVITGGGVGEVVESRSDAFQPGDIVTGQLGWQL
                     YSVMQGADGSIRKVDPTLAPVTTALGVLGMPGLTAYFGLLDIGQPVAGETVVVSGAAG
                     AVGTLVCQIAKIKNCRVVAIAGSDEKNRYLTSELGVDAAINYKTSTDMKAALREACPK
                     GVDLYFDNVGGDISDDVLPLINQRARIVICGQISLYNLEEPDVGLRVQPYLLVNSALM
                     RGFIVRDYAARAAEGIQQLGQWLAAGRLKYAENIVEGFENTPRAFLGLFTGENLGKQL
                     VKVAG"
     gene            2065..2418
                     /locus_tag="VGB76_05145"
     CDS             2065..2418
                     /locus_tag="VGB76_05145"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0098321.1"
                     /translation="MDVTQFQRIAKALADPRRFEILEVIASAGDEMCCGAVVECFPVA
                     QATVSHHLKELTDAGLIETRAEGQFKLLRARPEVIAEYIAELQRRVGAGRKTASNERG
                     AKSATATRQRKSAHL"
     gene            2546..2848
                     /locus_tag="VGB76_05150"
     CDS             2546..2848
                     /locus_tag="VGB76_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098322.1"
                     /translation="MNENIDLTPGLQCCLRCGRTLTDRALHDALEEPVLASIRAGRGV
                     GEGAEGECQPCLEEYRRLLVQRQARAERPLAESRGSRLPWVGKWLGKRAAAAGRAF"
     gene            2915..3259
                     /gene="trxA"
                     /locus_tag="VGB76_05155"
     CDS             2915..3259
                     /gene="trxA"
                     /locus_tag="VGB76_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0098323.1"
                     /translation="MSEHVREINDDSFEREVLQSEQPVLVDFWAVWCAPCRMMNPTVE
                     AVAAEYEGSARVVKLNVDDNPASSQRYGIKGIPTMILFRNGREEERVVGATSKDALSR
                     LLGKHVAVPGAV"
     gene            3265..4302
                     /locus_tag="VGB76_05160"
     CDS             3265..4302
                     /locus_tag="VGB76_05160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0098324.1"
                     /translation="MAEIIAQTPVRLSVLDLSPVPSGASSTEALRNTLDLARLADSLG
                     YTRYWLAEHHNTTLIASSSPEVMIGHVASVTEHLRVGSGGVMLPNHAPLRVAETFRML
                     EALHPGRIDLGLGRAPGTDSLTALALRRSRAALVADDFPEQLAELLAFFSGDFPADHP
                     YRHVRAIPQDAETPEIWLLGSSDFSARLAAERGLAFAFAHHINPATAINALQLYQERF
                     RPSAYLSAPCSMLAVSAVCAESDASAEELASSADLTLLRFQQGKFSGKLPSVAEARAH
                     SYSAYEREIVAANRARLFVGAPATVREQLLHLVRQTGVGEIMITTMTHAHEARRRSYE
                     LLAEAFALSPA"
     gene            4385..5392
                     /locus_tag="VGB76_05165"
     CDS             4385..5392
                     /locus_tag="VGB76_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0098325.1"
                     /translation="MKAYEVRQFGIENLAPVEREEPQAAAREVVVKFHAASLNYRDLM
                     FVKGTYHPKAKLPTVPFSDGAGEVVGVGRDVTRWKIGDRVCPTFLQGWFEGEPSMQKN
                     RTALGGGDLDGVLREYGAFDENGLVEIPASLSYEEAATLPCAALTAWNALVNSGNLKA
                     GDTVLTLGTGGVSIFAMQFAKIHGARVIATSSSDEKLERAKELGADVTINYRSAPDWD
                     KEVLSLTNRKGVDHVVEVGGAGTLGKSLNSVRVGGHVALIGVLAGGGDFDPRGMLMKS
                     VRLQGIFVGSRRMFEAMNGAIEANRVKPVIGRTFAFAEAREALRYMESGAHFGKIVLR
                     Y"
     gene            5566..>6475
                     /locus_tag="VGB76_05170"
     CDS             5566..>6475
                     /locus_tag="VGB76_05170"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0098326.1"
                     /translation="MIQTRGYAAAGATSPLAPFDFERRELGEQDVLIEILYCGVCHSD
                     IHQARNEWSNSIFPMVPGHEIVGRVTQAGSGVTRFKVGDLAGVGCFVDSCRVCGSCRE
                     GVEQYCEGHLVSTYNGTEKDEQTPTYGGYSKQIVMDENYTLKIPENLPLEGVAPLLCA
                     GITTYSPLRRFKVGAGQKVGVVGLGGLGHMGVKLAAAMGAEVTVFSTSHAKEADARRL
                     GAHHFVVTKDPQNLEPLANKFDFILDTVSAPHDLNMYLNLLGREKVMVLVGAPEKPAE
                     VAAFSLVTNGRTLAGSMIGGIRETQEM"
BASE COUNT         1324 a         1864 c         2085 g         1202 t
ORIGIN      
        1 cgtccggcct cgacgtgggc gactgcgtga gagtgcgcct gttgtcggtt gatgcgcggc
       61 gcggcttcat caatttcgcg cgcgtgctgt agtagcagcg cgcgaaggca gcgtgcggga
      121 tgacgaaagc gtgaacgtgt cgcagcgaaa tctgaaagaa gtctttgacg ctttagccgg
      181 atcgcccttt cgtcgccgct tccgcctgcg cggcaaagag cttgagtatc tgcgtcgcaa
      241 aggattaata gtggtcatgg agcacgcggc tgactttatc gaaaagcgtc tcgccccggc
      301 ctcgcccgcg cgcgatggta agcagacgcc gatgggtcat cacccggtct tcatcgcaca
      361 gcacgcgacc gccacctgtt gtcgcggatg tctacagaag tggcacggga tagccaaagg
      421 acgcgcgctc gaagcgacgg agcgggcata tattctcgaa gttatacgca gatggctggc
      481 acgcgaggaa gagttgaatg aaggaactac ttgagacata ccctctggtg atcgagatac
      541 ccgtcgcgtg gggagagatg gactctctcc gccacgtcaa caacatcgtc tattttcgtt
      601 acttcgagag cgcgcgcatg gcctactttc agaagttgga catctggaat tatatgaacg
      661 agacgggcat cggacctatc ctcgcctcga cgcaatgcag gttcaaaatt ccgctcacct
      721 acccggacat cgtctccgtg ggaacgaaga tttccgagat cgaagcggat cgtttcaaca
      781 tgaagtatgt ggtggtcagc cacgcgcacg cgaaggtcgc agccgagggc gaggggctta
      841 tcgtctctta cgattaccgc gcgcaaaaga aggccgcgct gcccgacgtg atcaaagagc
      901 gcatccgaat tttagaagac agcgcgcgga ggtgaagcgt ctgatgaacg ataaaacgaa
      961 caggaaaatc ttgcttgcca gccgtccggc gggaatgccg acgctcgaca acttcgagat
     1021 cgccgatgcc gggatgccgc aaccgcaagc gggagaggtt ctcatacgga cgctctacct
     1081 ctcggtcgat ccgtacatgc gcgggcgcat gagcgaacgc aaatcttata ttccgccctt
     1141 cgctctgaac gaggtcatca cgggcggcgg cgtcggcgaa gtcgtcgaat cgcgctccga
     1201 cgcttttcaa cccggcgaca tcgtcacagg ccaactcggt tggcaactct actccgtgat
     1261 gcagggcgca gacggctcga tcagaaaagt tgacccgacg ctcgcgcccg tcaccaccgc
     1321 gctcggcgtt ctcggcatgc cgggcttgac ggcctatttc ggtctgctcg atatcggcca
     1381 gcccgtcgca ggtgaaaccg tcgtcgtctc cggcgcggcg ggcgcggtcg gcacgctggt
     1441 ctgccagatc gctaagatca agaattgccg cgtcgtcgcc atcgccgggt cggacgaaaa
     1501 gaaccgttac ctgacgagcg aactcggcgt ggatgccgcg atcaactata aaacttcgac
     1561 tgacatgaaa gccgccttga gagaggcttg ccccaagggc gtcgatctct atttcgacaa
     1621 cgtcggcggc gacatatcgg acgacgtgtt gccgctcatc aatcaacggg cgcgcatcgt
     1681 tatctgcggc cagatctcgc tctataacct tgaggagccg gatgtgggtc tgcgcgtgca
     1741 gccgtatttg ctggtcaaca gcgcgctgat gcgcgggttc atcgtcaggg attacgccgc
     1801 gcgcgcggcg gaaggaattc aacagttggg acagtggctg gccgcaggcc gactcaagta
     1861 cgcggagaat atcgtcgaag gattcgagaa caccccgcgc gctttcctcg gcctattcac
     1921 gggagagaat ctcggaaagc aactcgttaa agtggccggt tgagagagcg tgcggttccg
     1981 ccgcaaggtt agcggcaatg cgcgcttgac aaaacatata gacggttgta tatatgtcgt
     2041 gtaatagatg agaggggtga ggctgtggac gtaacgcaat ttcagaggat cgccaaggcg
     2101 ttggccgacc cgcgccgctt cgaaatcctg gaggtgatcg cttcggcggg cgacgagatg
     2161 tgctgcggcg cggtcgtcga atgtttcccc gtggcgcagg cgaccgtgtc gcaccacctg
     2221 aaggaactga cggacgccgg gctgatcgag acgcgcgccg aagggcaatt caaactcctg
     2281 cgcgcccgcc cggaagtgat cgccgaatac atcgccgaac tccagcgacg cgtcggcgcc
     2341 ggacgcaaga cggcgagcaa tgagcgcggg gcgaagagcg cgaccgcgac ccggcagcgc
     2401 aagtcagccc atttatagtc agcccattta tagtcagggg gatgacatcg ggactttccc
     2461 gacaaacttt cgggattgat atagacgatt gtatatatgt acaacgcatg aagctaatcc
     2521 gtgacgcaag aggagggcac gcgccttgaa cgaaaacatt gatttgacgc cgggacttca
     2581 gtgctgcctg cgttgtggcc gcacgctgac ggatcgcgcg ctccacgacg ctttggagga
     2641 gccggtgctg gcctctatcc gcgcgggacg cggcgttgga gagggcgcgg agggcgagtg
     2701 ccagccctgc ctggaggaat accgcaggct gctggttcag cggcaggcgc gtgcggagcg
     2761 acccttggcg gaatctcgcg gcagccgcct gccgtgggtg ggaaagtggc tcgggaagcg
     2821 agccgccgcc gccgggcgtg ctttttaaga ggcgcgcatt gcgccgggga agtgaagcat
     2881 aagtttcagt gaacgaatgg aaggagtgat cgcaatgagt gaacatgtca gagagatcaa
     2941 cgacgattct ttcgagcggg aagtgctgca atcggagcag ccggtgctgg tggatttctg
     3001 ggcggtatgg tgcgcgccgt gccggatgat gaacccgacg gtggaagccg tggcggcgga
     3061 gtatgagggc agcgcgcggg tcgtcaaact caacgtggac gacaaccccg cgagttcgca
     3121 acgctacggc atcaaaggca tcccgacgat gatcctcttc cgcaacggtc gtgaagagga
     3181 acgcgttgtg ggcgcaactt caaaggacgc gctctcgcgc ctgctcggta aacacgtcgc
     3241 cgtgccggga gccgtctaaa gaccgtggcc gaaataatcg cgcagacacc tgttcgtctc
     3301 tccgtgctcg atctctcgcc ggttccgtcc ggcgcttcga gcacggaggc tctgcgcaac
     3361 acgctcgatc tggcgaggct cgcggatagt ctcggctaca cgcgctactg gctggccgaa
     3421 catcacaaca cgacgctcat tgcgagttcg tcgccggaag tgatgatcgg ccacgtcgcc
     3481 agcgtgacgg agcacttgcg cgtcggttcc ggcggggtga tgttgcccaa tcacgcgccg
     3541 ctcagggtgg ccgagacgtt ccgcatgttg gaggctttgc atcccggtcg aattgatctc
     3601 ggcctcggtc gcgcgccggg caccgattcg ctgacggcgc tggcgctcag gcgttcgcgc
     3661 gccgcgctcg tcgcagacga tttccccgaa caactcgcgg aactgttggc cttcttctcc
     3721 ggcgacttcc ccgcagacca cccttaccgt cacgtcagag ccattccgca ggatgccgag
     3781 acgccggaga tatggctgct cggttcgagc gatttcagcg cgcggctcgc ggcggaacgc
     3841 ggcctcgcct tcgccttcgc acatcacatc aatccggcga cggcaatcaa cgcgctccaa
     3901 ctctatcagg aacgtttccg gccatcagct tacttgagcg cgccgtgttc gatgctcgcc
     3961 gtctcagccg tctgcgcgga gagcgacgca tccgcagagg aactggcgag ttcagccgac
     4021 ctcaccttgc tgcgtttcca acagggtaaa ttttcgggga agttgccgag tgtcgccgaa
     4081 gcgagagcgc atagttacag cgcttacgag cgggagatcg tagcagccaa ccgcgcgcgt
     4141 ctcttcgtcg gcgcgcccgc gacggtgcgc gagcaactct tacatctcgt caggcagacg
     4201 ggcgtaggcg aaattatgat cacgacgatg acacacgccc acgaggcgcg gcgtcgttcc
     4261 tacgaactac tcgcggaagc gttcgcgctc tcgcccgcct gacaggtatg atggccgtca
     4321 atcactatgc cgaaataaaa gttgccggag taaaacttgt ctgcggcggg ttgcgaggag
     4381 cgagatgaag gcttacgaag tcagacaatt cggaattgaa aacctcgcgc cggtcgaacg
     4441 cgaagagccg caggccgccg cgcgcgaggt ggtcgtcaaa ttccacgcgg cctctctgaa
     4501 ctaccgcgat ttgatgttcg tcaaaggcac gtatcacccg aaagctaaac tgccgaccgt
     4561 gcccttttcg gatggcgcgg gcgaagtggt cggcgtcggg cgcgacgtga cgcgttggaa
     4621 aatcggcgac cgcgtgtgcc cgaccttcct gcaagggtgg tttgaaggcg agccttcgat
     4681 gcagaaaaac cgcactgcgc tcggaggtgg cgatctggac ggggtgttgc gcgaatacgg
     4741 cgcgttcgat gaaaacggtc tggtcgaaat tcccgcgtcg ctttcgtacg aggaagcggc
     4801 gacgttgccc tgcgcggcgt tgacggcctg gaacgcgctc gtcaactccg gcaacttgaa
     4861 agcgggcgac actgtcctga cgctcggcac gggcggcgtc tccatcttcg ccatgcagtt
     4921 tgccaaaatt cacggcgcac gcgtgatcgc aacttcgagc agcgacgaga aactcgaacg
     4981 ggcaaaagag ttgggcgcag acgtgacgat caactaccgg agcgcgccgg actgggacaa
     5041 agaggtcttg agtttgacga accgaaaggg cgtcgatcat gtcgttgaag tcggcggcgc
     5101 gggaactttg gggaagtctc taaactccgt gcgcgtcggc ggccacgtcg ctctgatcgg
     5161 cgtgctggcg ggcggcggcg atttcgaccc gcgtggcatg ctgatgaaga gcgtccgctt
     5221 gcagggcatt ttcgtcggct cgcgccggat gtttgaggcg atgaacggcg cgattgaagc
     5281 gaaccgggtg aagcctgtga tcggcagaac gttcgcgttc gcggaggcgc gcgaagccct
     5341 gcgctacatg gagagcggcg cgcacttcgg taagatcgtg ctccgttact aggagcttat
     5401 ttcaaaacta tgatcagccg tttaagcctt taagttctcc tttgcgcttc cggcaggaat
     5461 tgaactcttg ccggaggcgc aaagaggcga aggaaggcgg aaggtcgtcc ggcaactgcc
     5521 ggtagagtta caaaaatcaa cgggaagaaa gggaagaaac atctcatgat acagacacgc
     5581 gggtatgcag cggcgggggc gacatctccg ctcgcgccgt ttgatttcga gcggcgggaa
     5641 cttggggagc aggatgtgct catcgaaatt ctctattgcg gagtttgcca ctccgacatt
     5701 catcaggcgc gcaacgagtg gagcaactcg atctttccga tggtgcccgg ccatgagatc
     5761 gtcgggcgcg tgacgcaggc cggttccggc gtgacgcgtt tcaaagtcgg cgacctcgcg
     5821 ggcgtcggct gcttcgtgga ttcgtgccgc gtctgcggca gttgcaggga gggcgtggag
     5881 cagtattgcg aaggccatct cgtctccacc tataacggga cggagaaaga cgaacagact
     5941 ccgacctacg gcggctactc gaagcagatc gtgatggacg aaaactacac gctcaagatt
     6001 ccggagaatc ttccgctcga aggcgtcgcc ccgctgctct gtgcagggat caccacctat
     6061 tcgccgctgc gccgtttcaa ggtcggggca ggacaaaagg tgggagtcgt gggcttgggc
     6121 ggcctcggcc acatgggcgt gaaactcgcc gccgcgatgg gcgcggaggt gacggtcttc
     6181 agcacgtcgc acgcgaaaga agcggacgca cgccgcctcg gcgcgcatca cttcgtcgtc
     6241 acgaaagacc cgcagaacct cgaaccctta gccaacaagt tcgacttcat cctcgacacg
     6301 gtttccgcgc cgcacgatct caacatgtat ctcaacctgc tgggcagaga aaaggtgatg
     6361 gtgctcgtgg gcgcaccgga gaagcccgcg gaggtggcgg ccttctcgct cgtcaccaac
     6421 gggcgcacgc tcgccggttc gatgatcggc ggcatccgcg agacgcagga gatgt
//
LOCUS       DASWNS010000154         3074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_474599, whole genome shotgun sequence.
ACCESSION   DASWNS010000154 DASWNS010000000
VERSION     DASWNS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474599"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1532
                     /gene="hisS"
                     /locus_tag="VGB76_05175"
     CDS             129..1532
                     /gene="hisS"
                     /locus_tag="VGB76_05175"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY0098327.1"
                     /translation="MSKTQPARGMRDFLPADVRRREYVIRVIREVYERYGFEPLETPA
                     VENIETLLGKYGEEGNQLIYKILKRGEHEKSGEADLALRYDLTVPLARVVAEYGERLP
                     KFFKRYQIQPVWRADRPARGRFREFYQCDVDAIGSRSMIVEAELCAAASDALTQLGFT
                     NFTIRLNHRQVLAGVLEQAGIATELHGDALVALDKLDKIGAEGVAREFAERGIAEEAG
                     VKLLDFFASPASLERAAEIVAGESPERERAAYNAAVLGRLVEFLGDNETGARGADELR
                     QILGFANACGAGARVKVDPSLARGLSYYTGAIMEISVPDLAGSLGGGGRYDNLVGMFS
                     KQQVPACGFSLGLERIVVVMTEREMFPADLERASSDVLVTLWDAEAMTDALALARELR
                     RTGGLRVDVYPDGGDKVGKQFKYASARGVPFVVVAGPDERARDEVSLKDMSSGEQQTL
                     PRSAVGATLREKLGLKS"
     gene            1566..>3074
                     /gene="aspS"
                     /locus_tag="VGB76_05180"
     CDS             1566..>3074
                     /gene="aspS"
                     /locus_tag="VGB76_05180"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY0098328.1"
                     /translation="MLDNLGNLERTHSCGALRAEHVGQSVTLMGWVARRRDFGPLTFF
                     DLRDRDGVTQVVFNAETAPEAHAKAKQPRSEYVIAITGEVVLREEGQRNPKIRTGEVE
                     VQAREILILNDARTPPFQLEVAANETLAAEDLRLKYRYLDLRRPQLQENLRIRHRMLA
                     EIHRYMDEQGFTQIETPIMIKSTPEGARDYVVPSRTFPGKFYALPQSPQLFKQLCMIA
                     GLDKYYQIARCFRDEDLRADRQPEFSQLDVEMSFPTLERIYALTEGYFAGVFRAAVGI
                     ELSTPFPRLTYAEAMRRYGSDKPDLRFGMELQDVASAGALSDFPLFRETWNAGGEVKC
                     LVVTGGASQSRKRIDELTEFVKRYGARGLAWIKLADETGSSLLKALGAEAVEQLAQSV
                     GANRGDLVLIVADKSKTVAASLGALRIEIAKREKLIPEGVYAPLWVTEFPMFEYDEEA
                     DRYYAMHHPFTSALDEDLPLLERAVEGGETKLLGGLRAKAYDAVINGVEVAGG"
BASE COUNT          596 a          838 c         1047 g          593 t
ORIGIN      
        1 gaagaactac atcgaataca cggaacaata taaaaatgat gaatgatgaa tgatgaatta
       61 aggattcaag cgagcggcgt ctgattcttt ttctcatcat tcatcattca tcattcatca
      121 tttgctttat gtctaagacc caaccggcgc gcgggatgcg cgattttttg cctgcggatg
      181 tgcgacggcg cgagtatgtg attcgcgtca ttcgagaagt ctatgaacgt tacgggttcg
      241 agcctttgga gactccggcg gtcgagaaca tcgagacgct gctgggcaag tatggcgaag
      301 agggcaacca gctcatctac aaaatcctca agcgcggcga gcacgaaaag agcggcgagg
      361 ccgacctcgc cttgcgctac gatttgaccg tgccgctggc gcgcgtcgtc gcggaatacg
      421 gcgagcggct gccgaagttt ttcaaacgct accagattca acccgtctgg cgcgcagacc
      481 gcccggcgcg cggacgcttc cgcgaatttt atcaatgcga cgttgatgcc atcggctcgc
      541 gctccatgat cgtcgaagcg gaactctgcg cggcggcgtc ggacgctttg acgcaactcg
      601 ggttcacgaa tttcacgatt cgtctgaatc acaggcaagt gctcgcgggc gtgctcgaac
      661 aggcgggcat cgccacggaa cttcacggcg atgcgctcgt cgcgctcgac aagttggaca
      721 agatcggcgc ggagggcgtg gcgcgcgagt tcgcggagcg cggcatcgcg gaagaggcgg
      781 gcgtgaagtt gctggatttc ttcgccagcc cggcgtcgct ggagagggcg gcggagatcg
      841 tcgccggcga aagtccagaa agggaacggg cggcgtacaa cgccgccgtg ctcggtcggc
      901 tcgtcgaatt cttaggcgac aacgagacgg gcgcgcgcgg cgcggacgag ttgcgacaga
      961 ttttggggtt cgccaacgcc tgcggcgcgg gggcgcgggt caaagtcgat ccgagccttg
     1021 cgcgcggact ctcttattac acgggcgcga tcatggagat cagcgtgcct gatctcgcgg
     1081 gcagcctcgg cggcggcggg cgttacgata atctcgtcgg catgttctcg aagcagcagg
     1141 ttcccgcgtg cggtttctcg ctcggccttg agcgcatcgt cgtcgtaatg acggagcgcg
     1201 agatgtttcc ggcagacctt gagcgcgcgt cgtcggatgt attggtgacg ctgtgggacg
     1261 cggaggcgat gacggacgcg ctcgcgctcg cgcgtgagtt gcggcggacg ggaggtttgc
     1321 gggttgacgt ttatccggac ggcggcgata aagttggcaa gcagtttaag tatgcttcgg
     1381 cgcgcggcgt gccgttcgtc gtcgtcgctg gaccggacga acgggcgcgg gatgaagtct
     1441 cgctcaagga catgagttca ggcgagcaac agactttacc gcgcagcgcg gtgggggcga
     1501 cgttgagaga gaaactcgga ttgaaaagtt aggcgaagtt gagttcggat gttagggaat
     1561 atttcatgct cgataattta ggaaacctgg aacgcacaca ttcgtgcggt gctctgcgcg
     1621 cggagcacgt cggacagtct gtgacgttga tgggctgggt ggcgcggcgg cgcgacttcg
     1681 gaccactgac gttcttcgac ctgcgcgacc gcgacggcgt tacgcaggtc gtcttcaacg
     1741 cggagactgc gccggaggcg cacgcgaagg cgaagcagcc gcgcagcgag tatgttatcg
     1801 cgatcacggg cgaggtggtc ttgcgtgaag aggggcagcg gaatccgaag atcaggacag
     1861 gcgaggtgga ggtgcaggcg cgcgagattt tgatcctgaa cgacgcgcgc acgccgccgt
     1921 ttcaactcga agtcgctgcg aatgagacgc tcgcggcgga agatttgcgt ctgaagtatc
     1981 gttatctgga cttgcgccgc ccgcagttgc aggagaatct gcgcatccgc catcgcatgc
     2041 tcgcggagat tcatcgctac atggacgagc agggcttcac gcagatcgag acgccgatca
     2101 tgatcaagtc aacgcccgaa ggcgcgcgtg actacgtcgt gccgtcgcgc acgttccccg
     2161 gaaagttcta cgcgctgccg cagtcgccgc agttgttcaa gcaactctgc atgatcgcgg
     2221 ggctggacaa gtattaccag atcgcgcgct gcttccgtga cgaagacctg cgcgccgacc
     2281 gccagccgga gttttcccaa ctcgacgtgg agatgagttt ccccacgctc gaacgaattt
     2341 acgctctgac ggaaggatat ttcgcgggcg tctttcgcgc cgccgtcggc atcgagttga
     2401 gcacgccgtt cccgcgcctg acctacgcgg aagcgatgcg ccgttacggc agcgacaagc
     2461 ccgacctgcg tttcgggatg gagttgcaag acgtagcttc tgccggtgcg ctctctgact
     2521 ttccgctgtt ccgcgagacg tggaatgcgg gcggcgaagt taaatgtctc gtcgtaacgg
     2581 gcggggcatc gcaatcgcgc aagcggattg acgagttgac ggagttcgtt aaacgctacg
     2641 gcgcgcgtgg cctcgcctgg atcaagctgg cggacgaaac cggttcgtct ttattgaaag
     2701 cactcggcgc ggaggcggtc gaacaattgg cgcagagcgt cggcgcgaac cggggcgacc
     2761 tcgtgctcat tgtcgcggac aagtcgaaga cggtcgcggc gagcctcggc gcgttgcgca
     2821 tagagatcgc gaagcgcgag aagttgatcc cggaaggtgt ctatgcgccg ctctgggtga
     2881 cggagtttcc gatgttcgag tatgacgagg aggcggatcg ttattacgcg atgcaccacc
     2941 cgttcacctc cgcgctcgac gaagacttgc cactgttaga gcgggcggtc gaaggcgggg
     3001 agacgaaact gctcggcggc cttcgcgcga aggcttacga tgcggtgatc aacggcgtcg
     3061 aagtggcggg cggc
//
LOCUS       DASWNS010000155        35414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_214599, whole genome shotgun sequence.
ACCESSION   DASWNS010000155 DASWNS010000000
VERSION     DASWNS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 35414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35414
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214599"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VGB76_05185"
     CDS             complement(<1..553)
                     /locus_tag="VGB76_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098329.1"
                     /translation="MYRSRLSVIVGASLIFFTHSASAQSPATKLKAPLRNAAGTDKSD
                     ARAEADRIQKGRQSQARSLLFSLGGEARSFRDSKLRTRSLARIADALWDVDAEQGRVV
                     FREAWEAAETADRESKMRLNLRGEVLKLVARRDHLLGEEFLRKLKTAQAETESELSKV
                     NLWDLPEAAEKRLSLAENLLSTGD"
     gene            1535..2650
                     /locus_tag="VGB76_05190"
     CDS             1535..2650
                     /locus_tag="VGB76_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098330.1"
                     /translation="MSRRKLMLIFTVTIVALLVTAMLMPALPLVPASLAQTTSRDING
                     SSDRAPEDLDARFENAEAHDKESIEAAPYAADDSETTTDTSTAAAPGAKLYPIPVPLP
                     GGLGHVANGVGTRNAGYGTIRLRGVPPGSVRVGAYLYWGTIQNTPPPTQTILFNGVKV
                     TGRLIGTGQSCWCGGVFAAYRASVIALVNPAINADYRVSNMPSAVTNGSDPWNSPCLL
                     PLSQGASLVVAYAHNSIPPAARLFINDGAVFFANGAVNIFNQLPMPVVPYTRVRHTRL
                     GADGQVGGSTFSNLPITNERTFLGVPLTQIKGAGSPFNGDSDWNGDDGVPLNQLWDTH
                     TDSFNKALVPFGVFNYNVTYTANGDCIYAVAHILNTY"
     gene            3253..6381
                     /locus_tag="VGB76_05195"
     CDS             3253..6381
                     /locus_tag="VGB76_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY0098331.1"
                     /translation="MKLTKVLLLSLCLFMLVPQLARGQNLNSHGRLRKSDAAISDSYI
                     VVFNDSVAPARVQAFAAQLAREHRGTLGFTYQNAVLGFSVQLSEAQAVALSHNPHVAY
                     VEEDALVEGAATQTGATWALDRIDQRSLPLDTTYSYLNAGTGANVYVIDGGIRLTHQE
                     FGGRALFAYDNVGDGRNGVDCNGHGTHVAGVIGGKTYGVAKDAKLWAVRVLNCLNQGT
                     TARVLAGIDWVAANHVKPAVANLSFITVVGSDTIDLSVRKLVAAGVTTVVAAGNNTGE
                     ASLRSPARVTEAITVAASDQNDNQAPFSNYGAAVDVYAPGVDIVSASHFDDVSTSVRS
                     GTSSAAPFVAGLAARYLAARPGDPPAAVSQAIMNTATPDRVSGLGAGSPNLLAYAGIT
                     FGDDFNDNAHDASKWNAPYTGVNLAGAEFAPDQLPGTYGTHYIYPTRAEVDYFTGKGM
                     NVFRLPFLWERLQRTQFGDFDAAELARIDDFVNYASGRGASVVLDPHNYARYNNTVVG
                     MGIPTSAFADFWSKLASRYKGNSRVIFGLMNEPNGMATELWRDNANAAIQAIRNAGAA
                     NLILVPGNGWTGAHSWNHTWYGTPNSQVMLGINDPGNNYAFEVHQYLDSDFSGTSDQC
                     VSSTIGSEKLVEFTNWLRQHGKRGFLGEFAGGRNDTCAAALDNMLTYIDNNTDVWLGW
                     TYWAAGPWWEEYIFTLEPVNGADRPQMLPLTGHFASPSGGMPPPTPASGTGLKGDYYN
                     SISPTGAVAFTRTDATVNFDWGSDPGAGVSSDNFSVRWTGQVRPLYSETYTFYTVSDD
                     GVRLWVNGTQVINNWADHPATENSGTITLTAGQRYDIQMEFYERSGGAVSRLSWASPT
                     QAKQIIPASQLYPATVSQPNPPLVLDDFESGTLTKWNTFAGTGSNITGGVFAPAQSGN
                     YAMTVSYNVADWGGIEQVFNASRNWSGYTYLDFWFYGNNSGNRFRVELYDNGTSAGLA
                     ERFEYQFVDDASGWRRFTIPLNSFTRRADWQPSGAPNDGLTLTQMWGLNFSPLGGSNS
                     FRMDQVQLTYYS"
     gene            complement(6378..6866)
                     /locus_tag="VGB76_05200"
     CDS             complement(6378..6866)
                     /locus_tag="VGB76_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0098332.1"
                     /translation="MYEFLYGRAPRGQRVRESVPRNYGPQTSIISAVSLEGAEATTTV
                     EGAVDTDTFNAYIEQVLRPTIRPGDVLVLDNLSAHRASRLEIVAAECGAQVLWLAPYS
                     PDFSPIELMWSKIKTAMRSAKARTAEELNDALVAALQLVTRGDCSGWFTHCGYQVTLK
                     RN"
     gene            7264..7716
                     /locus_tag="VGB76_05205"
     CDS             7264..7716
                     /locus_tag="VGB76_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098333.1"
                     /translation="MPASRVTITALNCTLSLCGAQQLPLPALLHLSRASQHDLAALRE
                     LNPDLGACALFGDKAYADEETKTVFARRGTHLVTPDKRKRNEAVTAAPALWSRFVSGV
                     RQPLESLFGWLIQRTDIQDASRVRSTKGLLVHCYGKLAVARLLLTFYS"
     gene            complement(7764..10217)
                     /locus_tag="VGB76_05210"
     CDS             complement(7764..10217)
                     /locus_tag="VGB76_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0098334.1"
                     /translation="MNRSTLRLFMSMALLIIPDALSAQSLVSEAAIPVVNGSTFLLSQ
                     QSNDTRIVSGVILSEQNELVAGVTVLARSTTGQQRTTSDAEGNFRLSVPDGAVTLNFF
                     GRGIALVSKTIAAGEPMEDLQIKVAFIVPPVHESVVIMSSIVDPGVERRNDTIYRDGL
                     FLRDDQIFHTLDAGISAGQHEGGGKSVEIRRFGFNTDHGGVGGGLKVLVDDVQQNFGT
                     QGHGQGYLGQLKSLTPELVAGVDILNGPFSAEYGDFSGLGVVHIRTKESLPDEFAARL
                     QGGSFDTFRGFFAYSPKLDKASGFLAYEASRTDGPFISPLRYQRDNLTGNYTRTLDAN
                     SALGFKFNFGRNVFFSSGQIPLDEVAARRLDRFGFIDPDNGGRVRSGTVGMYYRRELG
                     GQSLFKIDGYVTRSLFDLWSNFTFFLADPEFGDEIQQHDSRLQEGANAQYLRPYTFSG
                     GRGLLTLGGNVQSFQTNVGLYPSIGRNPNRAEVTRASLTDCPFDGNPGQPGLPDKFNS
                     PCFLLTSVDARVTNTAGYIQQTLDLMGGHLHLEGGLRYDNFRFSVVDKIRLVFSGRQS
                     DGRLQPKASVAYVPSHSFPATFHFNYGRGISSQDARGVVQQPDNPKVATTDFYQIGTS
                     HNYKRFSLTTNLFLIDTSNQQVYIPDDGSIEFAGPGRSSGFEIKNSIAITGYLSFNGG
                     ITQVMNAFFRGTRPREYVDGAPHTVANASFTLSNYRGFAGSLRYRHTGNYRLDPLDAR
                     IRAVGLDVVDLYVSKRLRRFVDINFAVDNIFNKRYFETQNFFESRLRPGDAPAQRIHA
                     TPGYPSGVTIGLTFRFGEK"
     gene            complement(10480..11475)
                     /locus_tag="VGB76_05215"
     CDS             complement(10480..11475)
                     /locus_tag="VGB76_05215"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0098335.1"
                     /translation="MFKAKKILVTGAGGFIGHHLVKSLKSEGYWVRGVDIKYPEYEAS
                     HADEFALLDLRRSENCLRATSGVEQVYNLAANMGGIGFIESHKAEIVHDNVLINMHML
                     EAAKKNHLEKFLFTSSACVYPAYRQNIADAVPLKEEDAYPADAEDGYGWEKLYAERAC
                     RHYREDYGLKTYIVRFHNIYGPLGSFDGGREKSPAAICRKIALADDIDEIEVWGDGEQ
                     TRSYCYIDDCVEGITRLMKSDFHEPLNLGQDKMITINELVALVASIAGKKIKKWYDLS
                     KPQGVRGRNSDNTKLREVLDWEPQTSLEEGLENTYRWIQGELVSAGHLKERALCA"
     gene            complement(11509..12651)
                     /locus_tag="VGB76_05220"
     CDS             complement(11509..12651)
                     /locus_tag="VGB76_05220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEY0098336.1"
                     /translation="MQKGKQMKSLKIVPTHNSPAVTDAEPNDALKKTKPHLFDNSTLS
                     VIVRFHQSERLNFLEEALFSLALQNWSDLEPIIVLQNGTDDLKQTILDMLDHQPWQAS
                     PKYKIVSIQIPEGTDGRSVLLNRGIEAATGRYLSFLDDDDCVYHDGYTTLIRQLIRGE
                     RAVAVGGCRRAQIGYENDHWFTHKKDNFFAWGRTLTDLFAENFVPIHSYVIDRARLGN
                     AELKFDESFTLLEDYDFVLRLFAAHEPDFALLDKPVCEYRIRLDGSNSIAHTSAASPE
                     VIDLQQRAWLMIKESKKNLYGTYFSEKNINDFLESTTNPAGLESGDEAQDGVDRDRRV
                     LLKMVLMLYDFFSRYPRLERRLSKTLHLLWDTYKKLNGSASLDKQR"
     gene            complement(12756..14237)
                     /locus_tag="VGB76_05225"
     CDS             complement(12756..14237)
                     /locus_tag="VGB76_05225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0098337.1"
                     /translation="METVKDKLKDVQAAEAQPSALRDQLDETEAQFQIIARQWLDKER
                     QLQELAQLIKSRDQQHQALAESLNAYRQKVEELEQQAATREDLLAAKDWQLQAQESRL
                     EDQDRQFQEASRQLEESNYQLEAKAQRLSAVEKYARELVDSRAFRIGSTLTWPVRKLR
                     HSEWLRRSSHQFTDSEISLTSAETDEAETANAISFPQLIVELAERAIVREVKPVRNVG
                     TMAIGVVTYNNPPAHLAQLLRSIELSARRLNEPDISVRLYSIDNGAEALWPESGIPLT
                     RLASHGNIGFGRAMNLLMSDAFAEPETEWFLCVNPDGIIHHRALGELLSSSKAQPDSL
                     LEARQFPEEHAKSYDPVTLETSWASGACLLIRREIYETVGGFDPNFFMYLEDVDLSWR
                     VRAAGFSVKVSPHALFGHAVLEREYAPSTDATFLLSGRYLARKWKNSQFLRWAERELV
                     SRGYFRVKADLPPLPDPDDISDSEILSVQPDFNNYFNFSSPRW"
     gene            complement(14285..15613)
                     /locus_tag="VGB76_05230"
     CDS             complement(14285..15613)
                     /locus_tag="VGB76_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HEY0098338.1"
                     /translation="MKPIIRVENLGKQYGFGGLHPAYMTFREAAAAAVVKPFKRVRRR
                     GTQPATEERLWALKDVSFEIEPGEVVGLIGNNGAGKSTLLKILSRIVEPTTGTFELYG
                     RIGSLLEVGTGFHPDLSGRENIFLNGALLGIKRAELQRRFDEIVAFSEIEQFIDLPVK
                     WYSSGMYLRLAFSVAAHLNTEILTMDEVLAVGDISFQQKCIDKVNEIREQGRTILFVS
                     HSMAAVTRLCERVILLDKGRIVKDGAAAEVVNDYMGESLNVATAMREWPDPQSAPGNE
                     AVRIRRVRVRTKEGDTAPQVDIRSPVGIEMTYEVLQPDQILTPSFDLFNEQGVHVFAV
                     HDVGPEWRREPRPVGRYVSTMWIPGNTLSEGHLLVQASVLSYHPGLRLHTREPNVVTF
                     QVVESAEGDSARGDYVGAVPGVVRPLLPWQTDFYQTTDIALNRGKGLSGV"
     gene            complement(15621..16550)
                     /locus_tag="VGB76_05235"
     CDS             complement(15621..16550)
                     /locus_tag="VGB76_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098339.1"
                     /translation="MGSSGNEGVLASSVPPRRAPGTSAERASHPSLALPEHPLIKGGA
                     RTGGAWPSLNVRELWTYRELLYFLTWRDIKVRYKQTAMGAAWAVMQPFVTMLVFTLFF
                     SIFIGVPSDRIPYPVFVYAGLLPWTFFAGAVNNCSGSLVGSSALITKVYFPRMIIPAA
                     SVGAGLVDLAVAAVILMGLVFVYGLSPTWSLLMLLPLVMLTTVLAVGVGLLISALTVR
                     YRDVRHALPFVLQIWMFASPIIYPQSVVPEKWRWALTLNPLTGVIEGFRSALVGREFD
                     WPALGLATGISFALLIAGALVFRRLERVFADLI"
     gene            complement(16597..18924)
                     /locus_tag="VGB76_05240"
     CDS             complement(16597..18924)
                     /locus_tag="VGB76_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0098340.1"
                     /translation="MLLVIALAGSSAAHPLGQFTINHFSRLEVGTDRVNIRYVVDMAE
                     IPTFQELQTIGSNGNQASGAELENYVERAAARYADGLLLTIDGERVPLQVVARRISLP
                     EGAGGLRTLRIECDFAGKMPAASDTANQTRRLKFEDTNYRERAGWREAVVVPAAGVSI
                     FDSTAFGSALTDELKVYPEDLLAAPLDERAVELSFTQGAIPANAAALRARDGRPLGQA
                     RDRFAELISVRELTPAIALFGLLVAAALGALHAFSPGHGKTVVGAYLVGSRGTAKHAA
                     FLGLTVTVTHTAGVFALGLVTLFASQYIVPERLFPILGVVSGGIVLVMGLSLFASRLL
                     VALGLRSHDHGHTHGEEHAHEHTHDAAHSHTDGHAHTHDGDPTVPHSHGGGRAHTHLP
                     PGTDGSRVTWRSLLALGISGGLLPCPSALVVLLSAISLHRVGYGMLLVVAFSVGLAAT
                     LTAIGLAFVYAGRFILNRAGARINNLARVLPVASALVVACLGAALCYEALGQAGIHLS
                     DLLNSGVIGGETQAGGEQSLATMGAWAVLGLGLVFGLKHATEVDHIVAVTTIVSEQRN
                     LLRAALVGALWGAGHTISLIAVGIVVLALRVAIPELIADWLEFGVALMIIGLGLSAFV
                     RVLRRRRPDVHVHEHVHDDRPHAHIHFHEEGTEHTGSDTPHTHAVARLGLKPLLVGAM
                     HGLAGSAALTLLVLTQIASPLVGLLYLSVFGFGSILGMLLMSCLVGLPFVLGARKLTR
                     MSYALQATASVLSIAFGLWYAYEIGTASGLIGRII"
     gene            complement(19003..20316)
                     /locus_tag="VGB76_05245"
     CDS             complement(19003..20316)
                     /locus_tag="VGB76_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0098341.1"
                     /translation="MPKKLLTIAGIMLAGIAVVIGIRAVTKQPAPQAAPQREEARALD
                     QRATPADRHISTAQAAIARAPQSPDGYNQLAAAYTQKARETGDFGYYARVEAALKRSF
                     EVAPDNHDATRMQAYLLLAYHRFDEALPVARRAVEQKPKDYEAYGAVVDALVELGRYD
                     EAKKAVQAMLDVRPYTASYARASYLRSLYGDTEGAIEAMRLAVQSANPGDPESVAWCR
                     VHLGDELMNAGKRAEAEREYDHALFLFPDYHLALAAKARARLGANDTEGAVEFYKRAQ
                     ERVPSPETAIALGDIYTKSGRAEDAKKQFALVEFVESTGASGGTYSRQLALFWADHDM
                     KLDEALAVARRERETRSDIYTSDVLAWCLYKKGQYAEAKTAIDEALRLGTRDATINYH
                     AGMIYRALGERRAAAKHLQLALKLNPSFDVLQADVARQALRSLAA"
     gene            complement(20483..22117)
                     /locus_tag="VGB76_05250"
     CDS             complement(20483..22117)
                     /locus_tag="VGB76_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEY0098342.1"
                     /translation="MRRLLRAAHISLLIISMLAAGTFSTAVRASSHREAPSTSVDPVV
                     DGTDVYAFVDPNDPTKVNLIMNFIPFQLPQGGPNFYRFDDNVEYAMRIDNNGDAITDI
                     QYVFRFRTVVGNPNTFLYNTGPVTSLNDPDLNIKQFYSINVIRGNAPNTTTTTLTPTT
                     TAAGSPAGFPFAVPPVNVGPRSTPDYETNLAQPAVATISTASGDARVFAGQREESFFI
                     DVGAAFDLFALRPFNSAHLIPRTNTPGVDGLAGFNISTIAMQVPVATVTKTGAAPTGP
                     NDPNAVIGVYSASSRPAMIAFAEDRSGRATISGNFVQVSRLGNPLVNELIIPLGMKDR
                     FNASLPGNDSQFLNFVLDPEPGRLFGYPGAAGSPGKLFDVYPSFDAPAPPRSDIGVIF
                     LTGVGGLNAQTNTSGSPSVAAFPLQPPNTIPQERLRLNLAIAPSVGTNTGTGTGNRLG
                     FIAGDPAGFPNGRRLGDDVVDIELRVLAGVLGGNNVAPNNQLGDGVNQNDVPFMTVFP
                     YLATPRSGYDSPTSTAVGTTNTDAVAVVPPSPTPRP"
     gene            22681..23160
                     /locus_tag="VGB76_05255"
     CDS             22681..23160
                     /locus_tag="VGB76_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0098343.1"
                     /translation="MSSFTDNLTVRPLNSEDASDLSALLLAQTPAYVRFFTPFNFDLP
                     SVATLLAEQGTDVFMGMYWQDRLIGFFMLRGWNSGYKIPAYGVLIDEKYSGYGLTRLS
                     LKLAKSICKLRHVPRIMLKVNADNIIAKRLFEESGFTQTGVDAQTGNLIYHFDFRPH"
     gene            23252..24445
                     /locus_tag="VGB76_05260"
     CDS             23252..24445
                     /locus_tag="VGB76_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0098344.1"
                     /translation="MSNAEPGWRIVLLCAGVLPVIIKQLATGHPGLLEIELLPDPPAD
                     AQPRPLDKIGARFRRDKTPQLSWRYLQDWLARLELDLLHFPTQTLLHPDMQTPYCLTM
                     YGVQELPHLVPPVPHIITMHDVQELHFPENFTPAQRAIRAVQYWKALEKAGVVIVSFD
                     HVKQDLIKYFGLPEDKIRVCPIPFRDISLQEPTPAAAESYARKYEAWTPFLLYPAHTW
                     RHKNHFRLLQALRELRKDSGQNLKLICTGGTAHHYHAEVLAQVDEMKLSDAVLFSGIL
                     PEDELRWLYEQAALVTIPTKYEAGSFPLYEAMLLGSPVICSHVTSLPETIGDRRFVFD
                     PDDIESLASLIFRMLTDNVFRQANLANSATQAERLRRVNAAEHFYDAYRQLLAIPSGS
                     TLLFR"
     gene            complement(24492..25151)
                     /locus_tag="VGB76_05265"
     CDS             complement(24492..25151)
                     /locus_tag="VGB76_05265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0098345.1"
                     /translation="MEQKLFSIITPTYNCGPKVVRTINSVLSQDNGLYEYIVVDGDSN
                     DGTQSEIERFNAQIKFVSEPDTGIYDAMNKGIEMARGKYIYFLGAGDILRANALRNLA
                     GLMPDGELAFIYGNVYWVDEAFIYGGEFNKSRLETYNICHQAIFYERAIFDVIGRYEL
                     DYPILADYVFNIKCFGNDKIQKTYLDYVVANFEGGGQSARQKDAALIEALPRLIEEYL
                     R"
     gene            complement(25154..25645)
                     /locus_tag="VGB76_05270"
     CDS             complement(25154..25645)
                     /locus_tag="VGB76_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0098346.1"
                     /translation="MEIANLMFRPLRVEDASALSQWLRAQPPAYARFFHPFGYDEPAI
                     ADALARRGRDVFMGLFWREGIVGFFMLRGWNEGYEVPAFGILIDEQYRGHGLEMAALD
                     TAKVICRLRNVSRLMIKMHPDNISAKGVARKTGFVQTGVEATSGNLIYHYEIKPRAGT
                     STE"
     gene            complement(25694..26845)
                     /locus_tag="VGB76_05275"
     CDS             complement(25694..26845)
                     /locus_tag="VGB76_05275"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0098347.1"
                     /translation="MSALMLQEQVAERVETRRRRFIPVAETVLDGNEKKYVLDCLDTG
                     WISGSGKYVDDFEREFAAFCGVEHAVSVANGTAGLHVALLALGVGPGDEIIVPDLTYV
                     ASANAVTYCGAKPVLADVDSITWTLDPQDVARKISPATKAIMPVHLYGHPADLDPILE
                     LASAHNLSIVEDAAEAHGAEYKERRVGGLGDVGVFSFFGNKIITTGEGGMITTNDKVL
                     ADKIRRLKGQGMDPARRYWFPVVGYNYRMTNIQAAIGLAQLERIEWFIERKREVAALY
                     DEALRSLPVITPHESIRAKSVYWLYSICLPEECDRNQIMTRLLEQGIETRPFFHPMHH
                     LPPYYQRDGDSMLPIATNLAARGINLPSSALLTPDDITYITSALRACLR"
     gene            complement(26887..28176)
                     /locus_tag="VGB76_05280"
     CDS             complement(26887..28176)
                     /locus_tag="VGB76_05280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0098348.1"
                     /translation="MGNKEKIIRVGIFDQIVSGGGVRLFTLKLLEEFSRQAGSRWRFH
                     LMWPHFDSSNNFLAPPRLRNTTFERIRVGARSGVQDKFLHRVYEGFRGTKLSSTKRLS
                     SLKPVEAYEKTLREEEQQTFRSGTGIGLKWLDDRAHAFDLIYLPYPYLTLPRDVEWHP
                     KKPLVVTLHDLAHEHTDAWGEMTSSLQSEVRRWASLANLVIFSSTYIQNEAHRIYDLP
                     PGRTKRIYLTPPASVGSYGQTPGVLGRYGIKTDYVFTLGWAAKHKRVETIVEGFALFK
                     RRVETNLALVVAGPLTEILLGGNLHGLEVGRDLLSLGYVRDGDISALYQNASAVVTAS
                     ISEAGFNSMIFDAMYYEKPIICSNIPQFVERLGTDDRLALTFDPYSPQSLADALCKHF
                     GNLKESGQRTAEAKKFIDSRTLNHVAREYLESFESVL"
     gene            complement(28166..28741)
                     /locus_tag="VGB76_05285"
     CDS             complement(28166..28741)
                     /locus_tag="VGB76_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098349.1"
                     /translation="MKDVGTDEETVEVKLGQPKRVSTKAFLDLLNKGRSMVESGEIDL
                     NSPIEMEATAERSRSGKLSNIGINQKIIAAPQRELAKEFIEALSASGALEFLDGVRRI
                     VFTLRLNGDTLSLSASSELPSAKKAAEWAQASTLLFEVGASVKRGRDEEIIYQGASVS
                     ANGKRVTATLSLPRGTVADLLSKQLQSSSGQ"
     gene            complement(29123..29776)
                     /locus_tag="VGB76_05290"
     CDS             complement(29123..29776)
                     /locus_tag="VGB76_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0098350.1"
                     /translation="MSKLRIFLADDHAIIREGLKSLIEAQPDMIVVGETDNGRTAWRE
                     VKALQPDVVVMDISMPELNGAKATELLKRDCPQTKVLVLTVHNDQGYLHQLLKAGASG
                     YVLKRAAPHELIRAIRAVAGGETYLDPTIVSDVVGSYVRKQSTREGQPQGELSEREAE
                     VLRLIALGYSNKEIATRLDISVRTVETYKARLMEKLNFHSRAEIVRYALLQGWLDES"
     gene            complement(29773..32805)
                     /locus_tag="VGB76_05295"
     CDS             complement(29773..32805)
                     /locus_tag="VGB76_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0098351.1"
                     /translation="MEREECFSALCDRSPVGIFQSDAEGRCVYANARFRLICGFPSEK
                     CSGEEWGRFVHPEDRAESFAGWSADAHEGREYSGELRFQTPAGITRWVHIRTAPMFSK
                     EGVVVGHSGTAEDITARKKTELSLRESESRYRTLMEQASDGIHTYDFEGNFIDANTKL
                     CQMLGYTRDELLRLNVRDLIPVEDLKAAPLRLGNLRAGETVISERVVRCKDGTLLPVE
                     ISGRMLPDGVLQAIIRDITKRKQSEEAIRFQSNLLNTVEQAVIATDLSGRIIYWNRFA
                     EKLYGWPASEITGRSILEIVLPQAAQDQAVEIMSRLHRGQSWSGEFMVRRRDGTNFRA
                     FITDTPIYNDEGALIGIVGVSTDITERKRAEEALRESEERYRAIIDQAMVGVGQADLN
                     DNFTIVNQKYCDITGYPAAELLEMRIRDITHPDDMPLNVALRERMIAEGIPYEIEKRY
                     IHKNGSVVWVNLGVSAIRDRAGKPHSSIAVVLDITERKRAEEALRESEERLRIMFAAS
                     RDGILVEDDERIVYVNKSYAQLFGYDAPEELIGQHVSNIISTEDVERMLKFGSRRGSG
                     ERVISGYEFKGKRKDGTLVDVEASVSTSTVAGRTYITTMVRDITERKQTEEALRQAHD
                     QLERRVAERTAELAQINETLQAEIEERTHTEERLRDNEQRLKIAVQTGKLGLWELDLE
                     TGTLSCSDICKAHFGLPPEAEFSYDTLFAAVHPDDHARVGGAVEQALEKHTDYEAEYR
                     NLWPDGSAHWIITRGRGSYGADGQPTRMIGVTLNITERKESEEARRQLLRQLVTAQED
                     ERRRISRELHDQMGQHIVAIVLLVNSLKDFSRSEPATAAKFAQLENVAQHLSLQVDTL
                     AWKLRPTELDDVGLHAALRNYIGKWSKRYGLQVDFYGVSLADERLASEIETAIYRIAQ
                     EALNNIIKHARATHVSLILERRGNHVFTIIEDNGCGFDVEGLSAASVGQRRMGLLGME
                     ERTALVGGTLNIESSLGTGTTIFVRIPLALDENGGKSF"
     gene            complement(32982..33572)
                     /locus_tag="VGB76_05300"
     CDS             complement(32982..33572)
                     /locus_tag="VGB76_05300"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0098352.1"
                     /translation="MLYVVVGGIQWRMLPCNFPKWQSVYYYFRLWRKQNEWQRLHDRL
                     RAEVRRTAGRHKHPTAGCLDSQSVKTTQVPGVRGFDAAKNIKGRKRHLLVDTLGLLLS
                     VAVTAASVQERDGARLLLARRSGSCKKLRRIRVDGGYRGPSPALWVTAHFRFVLQVVL
                     RREEQLGFVVLARRWVVERTFAWLNLHRRLEIEKTS"
     gene            33932..34957
                     /locus_tag="VGB76_05305"
     CDS             33932..34957
                     /locus_tag="VGB76_05305"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0098353.1"
                     /translation="MILAVFTAVSAQTLDKAKLDQFFDRLAEKNKAMGSLTVARDGNI
                     LYTRAIGYSQINGSERKPATTATRYRVGSITKMFTAAMIFQLVEEGKLKLTDTLDKFF
                     PQIPNAKKITIAHILAHRSGIHDFIKEPDFRSWSMNPRTKDETLAFIAKGTPDFEPGE
                     KRSYSNAGYVLLGFVVEKLAGKPYQDALKKRITGKLGLKDTYAGTGKTDVSKKESFSY
                     SYAGDWKQHEEMDLSIPGGAAAIISTPSDLVKFIQALFDGKLISQENVNQMKNGMGMS
                     EHKLGDKTIYGHGGGTDGFRSTVLYLPEEKLAVAYTANAKVYSLESIFSGIFDIYYNK
                     PFEIPTF"
     gene            34982..35221
                     /locus_tag="VGB76_05310"
     CDS             34982..35221
                     /locus_tag="VGB76_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098354.1"
                     /translation="MEKYLGVYSSPGTPGKFTITREGATLHAQPTGQSSFPLEATAQD
                     KFKFDPVGVVMEFDAEKNQMILKRSGRETVFTKEH"
BASE COUNT         7530 a        10203 c         9891 g         7790 t
ORIGIN      
        1 tgtcacccgt actcaacaag ttctccgcga ggctcaatct tttctctgcg gcttccggaa
       61 ggtcccacaa gttgacttta gaaagttcgg attcagtctc agcctgagct gttttcagct
      121 tccgaagaaa ctcttcgccg agtaagtgat cacgtctggc taccagcttt aagacttccc
      181 cgcgcaggtt aagacgcatc ttgctttccc ggtcggctgt ctccgccgct tcccatgctt
      241 ctcggaaaac cacgcgcccc tgctcggcat caacgtccca cagcgcgtcc gcaattctcg
      301 ctagagagcg tgtgcgcaac ttcgaatcgc gaaaactgcg tgcctcgccg cccaatgaga
      361 acaggagcga acgggcttgc gattggcgtc ccttttgaat gcggtcggct tccgctctgg
      421 cgtcggattt atctgtgccg gcagcgttgc ggagaggagc tttcagcttc gtggccggtg
      481 actgggcgga agcagaatgt gtgaagaaaa ttaagctcgc accaacaatg acagacagac
      541 gggaacggta catggctact cctcgcaggt ggaatagact ctaaggtgga atagactcta
      601 agtgcgattc cacccatgct tactgaaaga atcaatcatg agtccgcacg aagctatcac
      661 ttcgatggtt tctaatgcaa tataaaatcg gtgctagaag actcaatatc ttgtctagtc
      721 ggaaagtttt tgcaacccga ccgttaccgc gagcgcatag caggagtcat ccatataata
      781 cgaatcgccg cacgaagcat agcgacaaat ctattcatag cagatcgcct ttttttcata
      841 ttgaaggctg aatagcatac gtatgaacat tcctattgac actaatttga ggcgagtgta
      901 acctgcgccc gttcctgtta agaacatgcg tcggcgagtc tcatccctcg ttggctgcac
      961 gattaagctc gtgaagggta aatcaactcg gttccttctg tagtagctat tctctcttaa
     1021 ataaataagc gcgcttaact tcgcccttgg aatttgttcg aggcgataac ggactgacaa
     1081 ccgttttcgt tttgcgcgaa gaggcataaa aatcaacgtc gcatgcgtgc gtgccgctac
     1141 tcgtgcggct gatgagcaat actgcctccg gttccctttg gctcagaagt gtatcgccgt
     1201 tagagctttc atattgtcgc agacaaaagc gactcaaccc ggacatacgc ccgttttcta
     1261 tgaaccgtag catctgccat cttatctcgt tgtctctgaa atcaacgtca ggcattgctg
     1321 acggctgcat cgtgtctaca ggccacaact gcccggtatt caaactctgt aactcactcc
     1381 gggcggaatg ctctgaaacg ctaaccatag cagactcttt ccgacgcgtt ataagtcaga
     1441 atttgaatgc tgctttctgt cctcaacctc acagttaatc cgaccttacg cggattactc
     1501 aggcgggagt aacgctcccg ataaggagaa cctcatgtcc agacgtaaat tgatgcttat
     1561 cttcacggtc acgatagttg ccctattagt gaccgcaatg ctgatgccgg ctttaccgct
     1621 tgttccggcc tcgcttgctc agaccacgag ccgtgatatt aacggttcat ccgaccgcgc
     1681 gcccgaagat ttggacgcaa gatttgagaa tgccgaagcg catgacaagg agtccattga
     1741 agccgcccct tacgcggctg acgatagcga aacaacgacc gacacttcca ctgctgccgc
     1801 gccgggtgcg aaactctatc cgattcccgt tccactgccc ggcggtctag gtcacgtggc
     1861 taacggagta ggaacgcgca acgcaggcta cggcactatc cggctgcgcg gcgtgcctcc
     1921 cggttcagtt cgcgtgggcg catatctcta ctggggcacg atccagaata ctccgccccc
     1981 aacccagacc attctcttta acggtgtgaa agtgacggga aggcttatcg ggacggggca
     2041 gagttgctgg tgtggcggag tcttcgccgc ctatcgcgcc agcgtcatcg cgttggttaa
     2101 ccccgcgatc aatgccgatt accgcgtctc caacatgccg agcgcagtca cgaatggtag
     2161 cgacccatgg aactctccgt gcctgctgcc actttcccag ggcgcgagcc tcgtggtcgc
     2221 ttacgctcac aacagcattc cgcctgcggc gcgactcttt atcaacgacg gcgcggtctt
     2281 cttcgctaac ggcgcggtca acattttcaa ccagcttccc atgccggttg taccttacac
     2341 acgagtcagg cacacgcgcc tcggcgccga tggtcaagtc ggcggcagca ccttctccaa
     2401 tcttccgatt actaatgaaa gaacgttcct cggcgttccc ctgacacaga ttaaaggagc
     2461 tggctcgccc ttcaatggtg attctgattg gaacggcgat gacggggtgc ccctgaatca
     2521 actctgggac actcataccg attcgttcaa taaagcgctg gtgccattcg gcgtgttcaa
     2581 ctataacgtc acatacacgg caaacggtga ctgcatctac gcggtggctc acatcttgaa
     2641 cacctactga aattgcagcg acgaaaagca tgaagtgagg cggcaggttc ccacgggtct
     2701 gccgcctcac tttgcgaggt ggcattgcga aaaatatttt ggccgcaatt agttgtactg
     2761 gagaatctgc cgccgcctca atatcttgtg tagtcggaaa gttttgcaac actaccattt
     2821 tttgcaacac tacacagaaa ccttgttatc ccattttgtt ttactctgaa tcatggcctg
     2881 ctctccaagt ttcattctga aacacccctt tcatcgttcc cttctttagc ttcctctaaa
     2941 gtcattgtcc catacggctt taagtttgag ggacgtgtgg aacggccttt gcactattga
     3001 tcgcgcaagc cggataaatt cttaatgtag agagctcaca gcaactctga tgaagtggga
     3061 ggccagacat tgttgcttcc ggctagcgtc gtctcagcca tttaacgacc aaatcacagg
     3121 tatagacggt tagcgcttcc gcgaagattt caggggtgag ggtgcgagac gattagggtg
     3181 cgacactgcc gcgcgtgtcg cgacgcaatt tcttcagaat tctttctcac aaaaaactca
     3241 taggggattt taatgaagct gaccaaagtt ctcctgctat cactctgcct gttcatgctg
     3301 gtgccccaac tcgcgcgtgg acaaaacctc aactcccacg gtcgcctccg caaaagcgac
     3361 gctgccatct ccgatagtta catcgtcgtc tttaatgaca gcgttgcgcc cgctcgcgtc
     3421 caagccttcg ccgctcaact ggcgcgcgag catcgcggta cgctcggctt cacctaccag
     3481 aacgccgtcc tcggcttctc cgtccaactg agcgaggcgc aggccgtcgc gctcagccac
     3541 aatccgcacg tcgcctacgt cgaagaagac gcgttggtgg agggcgcggc cacgcagacg
     3601 ggcgcgacat gggcactcga ccgcatcgac cagcgcagcc tcccgctcga caccacctac
     3661 tcatatctca atgcgggcac gggagccaac gtctatgtca tcgacggcgg cattcgcctg
     3721 acgcaccagg agttcggcgg gcgcgcactg ttcgcctacg acaacgtggg cgacgggcgc
     3781 aacggcgtgg actgcaacgg acacggcacg cacgtggcgg gcgtcatcgg cggcaagacc
     3841 tacggcgtgg cgaaggatgc gaagttgtgg gcggtgcgcg tcctgaactg tctgaatcag
     3901 ggcacgacgg cgcgcgtgtt ggcgggcatt gactgggtgg ccgcgaatca cgtcaaaccg
     3961 gcggtggcga acctgagttt catcaccgtc gtgggcagcg acacgattga cctgtccgtg
     4021 cggaagctgg tggcggctgg cgtcaccacg gttgttgcag cgggcaacaa cacgggcgag
     4081 gcgagtttgc gttcgcccgc acgggtgacg gaagcgatca cggtggcggc gagtgaccag
     4141 aatgacaatc aagcgccgtt ctcgaactac ggggcggcgg tagacgtgta tgcgccggga
     4201 gtggacatcg tctcggcgtc gcacttcgac gacgtttcaa cgagcgtgcg ttcgggcacg
     4261 tcgtcggctg cgccattcgt cgccggactc gccgcgcgtt atctcgccgc gcgtcccggc
     4321 gacccacccg ccgccgttag tcaggccatc atgaataccg cgacgcctga cagagtatcc
     4381 ggcctcggtg ctggctcgcc gaacctgctt gcgtatgcgg gcatcacctt cggcgacgac
     4441 ttcaatgaca acgcgcacga cgcgtccaag tggaacgcgc cctacaccgg ggttaatctc
     4501 gctggcgctg agttcgcccc tgaccaactg cccggcacct atggaaccca ttacatttat
     4561 cccacgcgtg ctgaagtcga ttacttcacc ggcaagggca tgaacgtctt tcgtctgccc
     4621 ttcttgtggg agcggctaca acgtactcaa ttcggtgatt ttgatgctgc cgaactagcg
     4681 cgcattgatg atttcgtcaa ttacgcgtcc ggcagaggcg cgtcggtcgt gctcgacccg
     4741 cacaattacg cgcgctacaa caatacggtc gtcgggatgg gtataccgac cagtgcgttc
     4801 gctgatttct ggagtaaact ggcgtcccgc tacaaaggaa acagccgcgt aattttcggt
     4861 ttgatgaatg agccgaacgg tatggcgacc gaactctggc gtgataatgc gaacgccgcc
     4921 atacaagcca ttcgcaacgc cggagccgct aatctgatac ttgttccggg caacggctgg
     4981 accggcgccc atagctggaa tcacacatgg tacggcacgc cgaactcgca ggtaatgctc
     5041 ggtattaacg accccggaaa caactacgct ttcgaggtgc accaatatct ggacagcgat
     5101 ttttccggta cgtctgacca gtgtgtcagt tcaacgatag gttcggagaa actcgtcgag
     5161 tttaccaact ggctcaggca gcacggcaag cgcggcttcc tcggcgaatt tgcggggggg
     5221 cgcaacgata cctgcgctgc cgccctggat aatatgttga cttacattga caacaatacg
     5281 gatgtctggc tgggttggac gtattgggcg gcaggaccat ggtgggagga atacatcttt
     5341 actttagaac ccgttaatgg tgcagaccgc ccgcagatgc tgcctttgac cgggcacttc
     5401 gccagccctt ccggcgggat gccacctcca acaccggcga gcggcacagg actcaaaggt
     5461 gactactaca attccattag ccctacgggc gcagtcgctt tcacgcgcac ggacgcaacc
     5521 gttaactttg actggggaag cgatccgggg gcgggcgtca gttccgacaa cttctcggtg
     5581 cgttggacag ggcaggtgcg acccctgtac tcggagactt ataccttcta caccgttagc
     5641 gatgacggtg tgcgcctgtg ggtcaatggc acacaggtca tcaataactg ggcggatcat
     5701 ccggcaacag aaaacagcgg caccatcaca cttactgccg gacaacgcta cgacattcaa
     5761 atggaattct acgagcgttc cggaggagcc gtgtcccgtc tctcgtgggc aagcccgacc
     5821 caagccaagc agatcattcc ggcgagccaa ctctacccgg cgaccgtctc acagcctaat
     5881 ccgccgcttg tgctcgatga ttttgaaagc gggacgctca cgaagtggaa tacatttgcg
     5941 ggcacggggt cgaacattac cggcggcgtc ttcgctccag ctcagtccgg caactacgcg
     6001 atgaccgtga gctacaacgt cgcggactgg ggcgggattg agcaggtgtt caatgccagc
     6061 cgcaactgga gcggctatac ctatctggac ttctggttct acggcaacaa ctcaggtaac
     6121 aggttccgtg tggaacttta cgacaacggc acttcggcag gtttggcgga acgcttcgag
     6181 taccagttcg ttgatgacgc aagcgggtgg agacgcttta ctattcccct taactccttt
     6241 acgcgccggg ctgattggca gccgagcgga gcgcctaacg acgggctgac gctgacgcag
     6301 atgtggggct taaacttctc acctttgggc ggcagcaact cattcaggat ggaccaggta
     6361 caactcactt actatagcta attgcgtttg agtgtgacct gataaccgca atgcgtgaac
     6421 caaccggagc agtcgcctcg ggtcacaagt tggagcgcag cgacgagtgc atcattcaac
     6481 tcttcggctg ttctggcttt ggccgaccgc atcgctgttt tgatctttga ccacatcaac
     6541 tcaatgggtg aaaagtccgg tgagtagggc gcgagccaca gcacttgtgc gccgcactct
     6601 gccgccacga tttccagtcg gctagcgcga tgtgccgaga ggttgtctaa caccagcacg
     6661 tcgcctggcc ggatagtcgg gcgcaagacc tgctcaatgt aggcattgaa ggtgtcggta
     6721 tcgactgccc cttcgactgt cgtcgtcgct tccgcaccct caagcgacac ggcgctgatg
     6781 atggaagtct gtggcccgta gttgcgtggt acgctctccc tgactcgctg gccgcgcgga
     6841 gcacgcccgt acaagaactc atacaactgt acctgttagt ctgcgacttc tacgataacc
     6901 agcccgactt gaaagatcaa cgcttgagca acttcaagcc gcgttgcaca gacgaagaac
     6961 tcgtcactat gtttttgttc ggtcacttgc aaggcttgca gcaacaacgg cgcatctatg
     7021 aataagtgcg tcgccactgg cagaagtggt ttcgcactgt gcccagttat caagccttca
     7081 accgccgctt aaacgaactc gttccgactt tcgagcacct gatcaccaag acgctctcta
     7141 ctggcgtcgg acaactcgcg ccgtcggctg accgcctgat tgattcgatg cctgtcatgt
     7201 tagccaaagg cacgaggagc agccgggcac gagtcgcggc ggaagtcgcg gacaaagggt
     7261 tttgtgccag caagtcgagt tactattacg gcgttaaact gcacgttatc gctttgcggc
     7321 gcacagcagt tgccgctgcc tgcgctcttg cacctgagcc gcgcttcgca gcacgacttg
     7381 gcggcgttgc gcgaactcaa cccagacttg ggcgcgtgtg ctctcttcgg tgataaagcc
     7441 tatgcggatg aagagacgaa aacggtcttt gctcgacgcg gcacacatct ggtgacgccc
     7501 gacaagcgaa agcgcaatga ggcagtgacc gccgctccgg cgttgtggtc gcgctttgtc
     7561 tccggcgtcc ggcaaccgct ggagagcctc ttcggctggt taattcaaag aaccgatatt
     7621 caggacgcgt cacgagtgcg ttcaaccaaa ggcttgctcg tgcattgtta cggcaaactc
     7681 gcggtggctc gtctgctctt aactttctac tcttgattca cattatgagt atgtatgcga
     7741 gcgctaacaa ctggcatctg ctgttatttt tctccgaatc taaaggtcag accgattgtc
     7801 acgccggagg gatagcccgg cgttgcatgg atacgttgcg cgggagcgtc gccggggcgc
     7861 aaccgcgact cgaagaaatt ctgcgtctcg aagtagcgtt tgttgaaaat attatcgacc
     7921 gcgaaattga tatcgacgaa gcggcgcaac cgtttgctga cgtagaggtc aaccacgtcc
     7981 aagccgaccg cgcggatacg tgcatcaagc ggatcgagac ggtagtttcc ggtgtgcctg
     8041 taacgaagcg acccggcgaa gccgcgatag ttcgagagcg tgaaactggc gttcgccacc
     8101 gtgtgcggcg cgccgtccac gtactcgcgc ggtcgtgtgc cccggaagaa ggcgttcatc
     8161 acctgcgtga tgccgccgtt gaaggataaa tagccggtga tcgctatcga gttcttgatc
     8221 tcgaagccgg atgagcggcc cggccccgcg aactcgattg agccgtcgtc aggtatatag
     8281 acctgctggt tcgaagtgtc gatcaggaac aggtttgtgg tcagggagaa ccgcttatag
     8341 ttgtgggacg tgccgatttg atagaagtct gtcgtcgcca ccttcgggtt atcaggttgt
     8401 tgcacgacgc cgcgcgcgtc ctgactactg atgccgcgcc cgtagttgaa gtggaaggtc
     8461 gccggaaagg agtgcgatgg cacgtaagcg acgctggctt tcggctgaag ccgcccgtcg
     8521 ctctgcctgc cggagaagac cagcctgatc ttgtcaacga cgctgaaacg gaaattatcg
     8581 tatcgcaggc cgccttccag gtgcagatgg ccgcccatga gatcgagcgt ttgctgtatg
     8641 tagcctgccg tgttggtcac acgcgcgtcc acactggtga ggagaaagca ggggctattg
     8701 aatttgtctg gcagtcccgg ctggccggga ttgccgtcga acgggcagtc cgtcagtgac
     8761 gcgcgcgtca cttccgcgcg gttcgggttg cgaccgatgg aggggtagag gccaacgttg
     8821 gtctggaagg attgtacgtt gccccccagc gtcagcagtc cacgcccgcc ggagaatgta
     8881 taggggcgta gatactgcgc gttcgctccc tcctgcaagc gcgagtcgtg ttgttgaatc
     8941 tcgtcgccga actccgggtc agcgagaaag aaagtgaagt tcgaccacag gtcgaagagc
     9001 gagcgggtca cgtagccgtc aatcttaaag aggctttggc caccgagttc gcggcggtaa
     9061 tacatgccaa ccgtgcctga acgcacgcgc ccgccgttat cggggtcgat gaacccgaag
     9121 cggtcgaggc gtctggctgc tacctcgtcg agcggtatct gccccgatga aaagaagacg
     9181 ttgcgcccga agttgaactt aaagccgagc gccgagttgg cgtcgagcgt gcgcgtgtaa
     9241 ttgccggtca ggttgtcgcg ctgatagcgc aagggactga tgaaaggccc gtcggtgcgc
     9301 gacgcctcat aggcgaggaa gccggacgct ttatccaatt tcggactgta agcgaagaag
     9361 ccgcggaagg tgtcgaacga gccgccttgc agacgcgcag cgaactcatc cggcagcgac
     9421 tctttagtgc ggatatggac gacaccgagg ccggaaaagt cgccgtactc tgcgctaaag
     9481 gggccgttca gaatgtccac cccggcgacg agttcgggcg tgaggctctt gagctggccg
     9541 agataccctt ggccgtgtcc ctgcgtgccg aaattctgct gcacatcatc aaccagaact
     9601 ttcagcccgc cgcctacgcc gccgtgatcg gtgttgaagc cgaagcggcg aatctccaca
     9661 gacttaccgc cgccttcgtg ctgacctgcg ctgatcccgg cgtccagcgt gtggaagatt
     9721 tgatcgtcgc gcagaaacag gccgtcccgg tagatcgtgt cgttgcgccg ctcgacgccc
     9781 ggatcgacga ttgacgacat gatgacaacc gactcatgca cgggtggcac gatgaacgcg
     9841 accttgattt gcaggtcttc cataggctcg cccgccgcga ttgtttttga gacgagcgcg
     9901 atgccccggc caaagaaatt gagagtcacg gctccatcgg gaacagacaa acggaagttg
     9961 ccttcggcgt cgctcgtcgt cctctgctgg ccggtggtcg agcgtgcgag gacagttacc
    10021 ccggcgacga gttcgttttg ctccgagagg atcacgccgg agacgatgcg ggtatcgttg
    10081 gattgttgag atagtaggaa cgtggagcca ttcacaacgg ggatggctgc ctcgctgact
    10141 aatgactgcg cgctcagcgc atcagggatg atcagcagcg ccatgctcat aaagagtctc
    10201 aatgttgatc tattcataac tactcactgt ggaaaatgtt cttccgtaag atatcaggcg
    10261 cacggcaata ttacgcctca agcggtttcc agagtgcaaa cacgcttaac tttcaacgga
    10321 accggattca tgtcagggat cggccagcgg ttaccactcc ggcgaggtta gagttatgag
    10381 agcaatatta actatgggtg gaggcagcgg ttcggataag ggaaattact cactgacgat
    10441 cgaactttag cttgttgccg aaccacttga gattgatggc tacgcgcaaa gcgcacgttc
    10501 ctttaaatgt cccgcgctga ccaactctcc ttgaatccat ctgtaagtgt tttccagccc
    10561 ttcctccagc gaagtctgtg gctcccagtc gagcacctcg cgcagcttcg tgttatcgct
    10621 atttctgcca cgcacgcctt gaggcttcga caggtcgtac cacttcttga tcttcttgcc
    10681 ggcaatgctc gcgactagtg cgaccagttc gttgatcgtg atcattttgt cttgtccgag
    10741 attgagtggt tcgtggaagt ccgacttcat caatctggtg atgccctcga cacaatcgtc
    10801 tatgtaacag taagagcgcg tctgctcacc gtctccccag acttcgatct cgtctatgtc
    10861 gtcggcaagc gcaatcttgc gacaaatcgc cgccggcgat ttttctctcc cgccgtcaaa
    10921 gctgccgaga gggccgtaga tgttgtgaaa gcgaacgatg taagttttga gtccgtagtc
    10981 ttcgcggtaa tgccggcagg ctctctccgc atacagtttt tcccaaccgt aaccgtcctc
    11041 cgcgtcggct gggtaggcgt cttcctcttt gaggggaacg gcatccgcga tgttctggcg
    11101 ataagcggga tagacgcagg cggaagacgt gaagaggaac ttctccagat gattcttttt
    11161 tgcggcctcc agcatgtgca tattgatgag aacattgtcg tgcacgatct cggctttatg
    11221 actttcgata aaaccgatcc cgcccatgtt ggctgcaaga ttgtaaactt gctcgacgcc
    11281 cgaggttgcg cgcagacagt tttcactcct tctcaaatcc agcaacgcaa actcgtctgc
    11341 gtggctggcc tcgtattccg ggtactttat atccacgcct cttacccagt agccttccga
    11401 cttcagggac ttcaccaagt ggtggccgat aaacccgccg gccccggtca caagtatttt
    11461 tttcgcctta aacatgtcac acctaacttc gggcttcgct tttgttcgtc acctttgttt
    11521 atcgagagac gcgctgccat taagtttttt gtacgtgtcc cacaggagat gcagtgtttt
    11581 actaagccgc cgttccaggc gcgggtagcg gctgaaaaag tcgtacagca tgagaaccat
    11641 tttcaggaga acccgcctat ctcgatcaac gccgtcctgc gcctcgtcgc cggactctaa
    11701 accggcggga ttagtcgtgg actccaaaaa atcattgatg tttttctcgg agaagtatgt
    11761 accgtaaagg tttttcttgc tctctttaat catcaaccag gcgcgctgct gtaaatcaat
    11821 gacctccggg gaggcggcgg aagtgtgcgc gatgctattc gagccgtcga gacgtatcct
    11881 gtattcgcag acaggtttgt ccagtaaggc gaagtccggc tcgtgcgctg cgaacaggcg
    11941 caacacgaag tcgtaatcct ccagcagggt gaaagactca tcgaacttca actccgcgtt
    12001 gccgagccgc gcacggtcaa tcacgtacga gtggatgggc acaaaatttt cggcgaacag
    12061 atcggtgagc gttcgacccc acgcgaaaaa gttatctttc ttgtgtgtaa accagtggtc
    12121 gttttcgtaa cctatctgtg cccggcggca accgccgacc gcgacagcgc gttcacctct
    12181 gattagttgc cggatcaatg tcgtgtaccc gtcgtgatac acgcagtcgt cgtcgtcgag
    12241 gaaagacagg tatctgcccg tcgcagcctc gatgccacgg ttcagtagaa cgctgcgtcc
    12301 gtcagttcct tcaggtatct ggatagacac gattttatac tttggagatg cctgccacgg
    12361 ttgatgatcc agcatgtcca gaatcgtctg ctttagatca tcggttccgt tttgcagcac
    12421 gatgataggt tcgagatcag accaattttg gagcgccaga gaaaagagcg cctcttccag
    12481 aaaattaagc ctctcgctct ggtgaaagcg aacgatgacg ctgagagtgc tgttatcaaa
    12541 gaggtgtggc ttggttttct tgagcgcatc gttcggctcc gcatcggtga cagcaggact
    12601 gttgtgagtc ggaactattt ttaatgattt catctgcttg cccttctgca ctcaactaaa
    12661 actccgcccc gcatgtctcg gagacaacca ctctgacgtg agaaacttgg ccgctacatc
    12721 tctgctcaat gcttgcctga tggtttgcag gcaactcacc acctcggcga cgaaaagtta
    12781 aaataattat tgaagtccgg ttggacggat aagatttcgc tatcgctaat gtcgtcagga
    12841 tcaggcaacg gcggcaaatc agccttgaca cgaaaataac cgcgactgac gagttctcgt
    12901 tcagcccaac gaaggaattg cgaatttttc catttgcgcg ccaggtatct tcccgacagg
    12961 agaaaagtcg cgtcggtgct cggagcatac tcgcgctcaa gcacggcatg cccgaataac
    13021 gcgtgcggcg aaaccttcac ggagaacccc gccgcgcgaa cgcgccacga gagatcaacg
    13081 tcttccaggt acatgaagaa gttagggtcg aagccgccga ccgtctcgta tatctcgcgg
    13141 cggatgagca gacacgcgcc cgacgcccac gacgtttcca gcgtcacagg gtcataagac
    13201 ttggcgtgtt cttccgggaa ctggcgcgct tcgagcaggc tgtcgggctg cgccttgctg
    13261 ctcgacagca gttcgccgag cgcgcggtga tggatgatgc cgtccgggtt gacgcagagg
    13321 aaccattcgg tttcgggttc ggcaaaggca tctgacatca agaggttcat ggcgcgaccg
    13381 aaaccgatat tgccgtgtga agcgaggcgt gtcagaggga tgccggactc cggccagaga
    13441 gcctcagccc cgttatcaat cgaatataga cggacactga tgtccggctc gttaaggcgg
    13501 cgcgcggaca gttcgattga gcggagcaat tgtgcgagat gggcgggcgg attattgtag
    13561 gtcacgaccc caatcgccat cgtgccgaca ttcctcaccg gcttcacctc tcgaacaatc
    13621 gctcgctcag ccagttcaac tatcaattgc ggaaatgaga ttgcgttcgc cgtctcggcc
    13681 tcgtcagttt ccgcgctcgt caaactgatt tccgaatcgg taaactggtg ggaactgcga
    13741 cgaagccatt cgctatgtcg tagcttgcgt accggccacg tcaaagtcga accgattcta
    13801 aaggcacgcg aatcaaccaa ctcccgcgcg tatttctcaa ccgccgagag gcgctgcgcc
    13861 ttcgcttcga gttggtaatt tgactcttca agttggcggc ttgcctcctg aaactgccgg
    13921 tcttgatctt cgaggcgaga ttcctgcgct tgcagttgcc aatctttcgc cgcgagcagg
    13981 tcttcacgtg tggcggcctg ttgttccagt tcctctactt tctgccggta ggcgtttaag
    14041 gattcggcga gcgcttggtg ctgttgatct cgactcttga ttagttgcgc caactcttga
    14101 agctgccgct ctttgtcgag ccactggcgc gcgatgattt ggaattgggc ttcggtttca
    14161 tcaagctgat cacggagcgc cgaaggctgt gcctccgccg cttgaacatc cttgagctta
    14221 tctttaactg tctccatttt cttaacaccc atcaacaagt cgcacaccgt caggcaataa
    14281 tcatttagac acctgacaat cctttgcccc tgttcagagc gatgtccgtc gtttgataga
    14341 aatccgtctg ccagggcagc agcggcctca cgacacccgg aaccgcaccg acataatccc
    14401 cacgcgccga atccccttcc gcactttcga cgacttgaaa cgtgacgacg ttcggctctc
    14461 gcgtgtgcaa acgaaggccg gggtgatatg acaggacaga ggcttgcacc aggagatgac
    14521 cttcggaaag tgtgtttccg ggtatccaca tcgtgctgac gtaacgcccg acgggtctcg
    14581 gctcacgacg ccactccggc ccgacatcgt ggacggcgaa cacatgcacg ccttgctcgt
    14641 tgaacaaatc gaagctggga gtcagtatct gatccggttg gagcacttcg taggtcatct
    14701 cgatcccgac ggggctgcgg atgtccactt gcggcgcggt gtcgccttcc ttcgtccgca
    14761 cacgcacccg gcggatacgc accgcctcgt tccccggcgc gctctgcggg tcgggccact
    14821 cccgcatggc ggtcgcgaca ttcaagctct cgcccatgta atcgttgacg acttccgccg
    14881 ccgcgccgtc tttcacgatg cgccccttgt ccagcaagat gacgcgttcg cacaggcgag
    14941 tcacggccgc catgctgtgc gagacgaaca ggatggtgcg cccctgctcc ctgatctcgt
    15001 tcaccttgtc aatgcacttc tgctggaacg agatgtcgcc gacggccagc acctcgtcca
    15061 tcgtcaatat ctccgtgttg aggtgcgccg cgaccgagaa ggcgaggcgc aggtacatcc
    15121 cgctcgaata ccacttgacg ggcaggtcaa taaactgctc gatctcggag aaggcgacga
    15181 tctcgtcgaa tctgcgttgg agttcggcgc gcttgattcc taagagcgcg ccgttcaaaa
    15241 aaatgttctc gcgcccggaa aggtctggat gaaaacccgt gccgacttcg agcaggctgc
    15301 cgattcttcc gtacagctca aaagtgcccg tggtcggttc gacgatgcgc gaaagaattt
    15361 tgagcagcgt ggacttgccc gccccgttgt tgccgatgag gccgacaacc tcgcccggct
    15421 cgatctcgaa ggaaacgtct ttgagcgccc acagtctttc ttcggtcgcg ggctgcgtgc
    15481 cgcgacgccg tacgcgtttg aagggcttga ccaccgctgc cgctgccgcc tcgcgaaacg
    15541 tcatgtaagc cgggtgcaat cctccgaagc cgtattgctt gccgagattt tcaactctga
    15601 tgattggttt catggcagcc tcaaatcaaa tcggcgaaga cgcgctccag ccgccggaac
    15661 accagcgcgc ccgcgatgag caacgcgaac gatatccctg tggcgaggcc gagcgcaggc
    15721 cagtcgaact cgcgcccgac gagcgcggaa cgaaaacctt cgatcacgcc ggtcagagga
    15781 ttgagcgtca gcgcccaacg ccacttctcc ggcacgacgc tctgcgggta gatgatcggc
    15841 gatgcgaaca tccaaatctg taagacgaag ggcagcgcgt gccggacatc ccgataccgt
    15901 accgtcaggg ctgaaatcaa aagccccacc ccgacggcta acacggtggt gagcatcacg
    15961 agcggcagca gcatcaacag gctccacgtc ggagacaggc cgtagacgaa caccagcccc
    16021 atgaggatca cggcggccac cgcgagatca accaaccccg cgccgacgga ggctgccggg
    16081 atgatcatgc gcgggaaata aaccttcgtg atgagagcgg aactcccgac gaggctgccg
    16141 ctacagttgt tgaccgcgcc cgcgaaaaac gtccacggca gcaaccccgc atagacgaaa
    16201 accgggtacg ggatacggtc ggacgggacg ccgatgaaga tgctgaaaaa gagcgtgaag
    16261 accagcatcg tgacgaacgg ctgcatcacc gcccacgccg cccccatcgc ggtctgcttg
    16321 tagcgcacct tgatgtcgcg ccacgtcagg aagtaaagca actcgcggta cgtccacaac
    16381 tcgcgaacat tgagcgacgg ccaagctccg ccagttctcg ccccgccctt gatcagcgga
    16441 tgttccggta aagctaaaga gggatgcgat gcgcgctcgg cgctcgtccc cggcgcgcgg
    16501 cgcggcggga cggacgatgc cagcacgccc tcattaccgc tgctccccat tagcttgctt
    16561 tctgccgaca acgctctgcc cccttgtcgc cgaatttcaa attattcttc ctatcaagcc
    16621 gctcgcagtt ccaatctcgt aggcgtacca caatccgaat gcgatgctta gaacgctcgc
    16681 cgtcgcctgc aaggcgtaac tcatccgcgt gagtttgcgc gcgccgagca cgaatggcag
    16741 acccaccaga catgacatca acagcatgcc aaggatcgag ccgaaaccga agacggacag
    16801 gtacagcagc ccgacgagcg gcgatgcgat ctgcgtgagc accaacagcg tcaacgccgc
    16861 cgaacccgcc aacccgtgca tcgcgccgac gagcagcggc ttcaagccca gacgcgcgac
    16921 ggcgtgcgtg tgcggcgtgt cggagccggt atgttccgtc ccctcttcat gaaagtgaat
    16981 gtgcgcatgt ggccggtcgt cgtggacgtg ctcgtgcaca tgaacatccg gacggcgacg
    17041 ccttagcaca cgcacaaatg cgctcaaccc cagcccgatg atcatcaacg ccacgccgaa
    17101 ctccagccag tcggcgatga gttcggggat cgccacacgc aaagcgagga cgacgatgcc
    17161 gacggcgatg agtgaaattg tgtgccccgc tccccataac gcgccgacga gcgcggcgcg
    17221 gagcaagttg cgttgttcac tcacgatggt ggtcacggcg acgatgtggt caacttcggt
    17281 ggcgtgctta agtccgaaga ccagcccgag gccgagcacc gcccaagccc ccatcgtagc
    17341 cagcgattgc tcgccgcccg cctgcgtctc gccaccgatg acgccggaat tcagtaagtc
    17401 gctcagatga atgcccgctt gtccgagagc ttcgtagcag agcgccgcgc cgaggcatgc
    17461 cacgaccaac gcgctcgcaa ccggcaggac gcgcgccaga ttgttgatcc ttgcccccgc
    17521 gcgattcaag atgaagcgtc cggcgtaaac aaatgccagc ccgattgccg tcagcgtcgc
    17581 ggccagtccc acgctgaagg cgacgacgag cagcatgccg tagccgacgc ggtgcagcga
    17641 gatcgccgac agcagcacga ccaacgccga aggacagggc agcaagccgc ccgaaatgcc
    17701 gagcgcgagc aggctgcgcc acgtcacacg gctgccgtcg gttccgggcg ggagatgcgt
    17761 gtgtgcccgc ccgccgccgt gcgaatgcgg cacggtcggg tcgccgtcgt gcgtatgcgc
    17821 gtgcccatct gtgtggctgt gcgccgcatc gtgcgtgtgt tcatgcgcgt gctcttctcc
    17881 gtgcgtatgc ccgtgatcgt gtgacctcag gccgagcgcg acgagcaggc gactcgcgaa
    17941 taaactcagt cccatcacca gcacgatccc gcccgaaacc acgccgagga tcgggaacaa
    18001 acgttcgggg acgatgtatt gcgaagcgaa cagcgtcacg agtccgagcg cgaagacgcc
    18061 cgccgtgtgc gtgacagtca cggtcaggcc gaggaaggcc gcatgcttcg ccgtcccgcg
    18121 cgagccaacg aggtatgcgc ccacaaccgt cttgccgtgg ccgggcgaaa aggcgtgcag
    18181 cgcgccgagt gcggcggcta cgagcaggcc gaaaagggcg atagccggcg tcaactctcg
    18241 cacgctgatc aactccgcga agcgatcccg cgcctgcccc aacgggcgac cgtcgcgtgc
    18301 gcgcagggcg gcggcattcg ccggaatcgc gccctgcgtg aacgagagtt ccacggcgcg
    18361 ctcatcgagc ggcgcggcca gcaagtcttc cgggtaaact ttcaactcgt cggtcaatgc
    18421 cgagccgaac gcggtgctgt caaaaatgga aaccccggcg gcggggacga ccacagcctc
    18481 tcgccaaccc gcgcgttcgc ggtaattggt atcctcaaat ttgagacggc gcgtttgatt
    18541 tgccgtatcg ctcgcggcgg gcatcttgcc cgcgaagtca cactcgatgc ggagcgtgcg
    18601 caggccgccc gcgccctcgg gcagcgaaat cctgcgcgcg accacttgca gcggcacgcg
    18661 ctccccgtca atcgtgagca acaagccgtc ggcgtagcgc gcggcggctc gttcgacgta
    18721 attttccaac tccgcgccgg atgcttgatt accgttgctg cctatcgtct gtaactcttg
    18781 aaacgtggga atctccgcca tgtcaacgac gtagcgaatg ttgacgcgat ccgtgccgac
    18841 ttcgagacgg gagaagtgat tgatcgtgaa ttgcccgagc gggtgagcgg cggacgagcc
    18901 tgccaacgcg atcacaagaa gcatgacaga gcaggttatt ttggcggcgc gacgtgcgcg
    18961 agtcggctgc gaacggtgga acattaatgt cacctcaact cctcaggcgg cgagagagcg
    19021 caacgcctgc cgcgcaacgt ctgcctgtag tacgtcgaat gaaggattga gcttgagcgc
    19081 gagttgcagg tgtttcgcgg cggcacgtcg ttcgccgagc gcacgataga tcatccccgc
    19141 gtgatagttg atagtggcat cgcgcgtgcc gagccggagc gcctcgtcaa tggcggtttt
    19201 ggcctcggcg tactgcccct ttttgtagag acaccacgcc agcacatcgc tcgtgtaaat
    19261 gtccgagcgc gtctcgcgct cacgacgggc gacggcgaga gcctcgtcga gtttcatgtc
    19321 gtggtcagcc cagaagaggg cgagttgacg cgagtaagtg ccgcccgatg ctcccgtgct
    19381 ctcgacgaat tcgactaacg caaactgctt tttcgcatct tcggcgcgac ctgactttgt
    19441 ataaatgtcg ccgagcgcaa tcgcggtttc gggagacggg acgcgctcct gtgcgcgctt
    19501 gtaaaactct actgcgccct ccgtgtcgtt cgcgccaaga cgcgcgcgcg ctttggcggc
    19561 gagcgcgagg tgatagtccg ggaataagaa gagcgcgtgg tcgtactcgc gttccgcctc
    19621 ggcacgcttg cctgcgttca tcaactcgtc gcccagatga acgcgacacc acgcaaccga
    19681 ttccgggtcg cccggattag ccgattgaac ggcgaggcgc atcgcttcta ttgcgccctc
    19741 cgtgtctccg tacagcgagc gcaggtaaga ggcgcgggcg tatgatgcag tgtatgggcg
    19801 cacgtcgagc atggcctgca cggccttctt agcctcgtcg tatcggccta gctcaacgag
    19861 cgcatccacg accgcgccgt atgcctcgta gtccttcggc ttctgctcga cggcgcggcg
    19921 cgccaccggc aacgcttcat cgaagcggtg gtaggcgagc aggagatagg cttgcattct
    19981 ggtcgcgtcg tgattgtcgg gtgcgacctc aaacgagcgt ttgagcgccg cttccacgcg
    20041 cgcgtaatag ccgaagtcgc cggtttcgcg tgccttctgc gtgtaagcgg ctgcaagttg
    20101 attgtatccg tcgggagatt gtggcgcgcg ggcgatggca gcctgtgccg tgctgatgtg
    20161 gcggtcggcg ggcgttgcgc gttgatcgag ggcgcgtgcc tcttcccgtt gcggcgcggc
    20221 ttgtggcgcg ggctgtttcg tcacggctct gatgcctatc acgacggcga ttcccgcgag
    20281 catgatccct gcgatggtca ggagtttctt cggcataact tattctgctg tggttaagat
    20341 gcggaaggca ggagccggat catttagtga tccggctcct gcgcgatgaa cgtcaacaag
    20401 cgtttaactg tacgcgctga tagagcggag ctaggtgtta cgcacactgc ttgcctgccg
    20461 gcgcgcgtgc cctgtcgccc gtttacggtc tgggcgtcgg cgaaggcggc acgactgcca
    20521 ccgcgtccgt attggtcgtc cccacggcgg tactcgtcgg cgagtcatag ccggagcgcg
    20581 gcgtcgccag atacgggaac accgtcataa acggcacgtc gttttgatta acgccgtcgc
    20641 cgagttggtt attcggtgcg acgttgttgc cgcccaacac tcccgccagc acccgcaact
    20701 caatgtcaac cacgtcgtcg ccgaggcgac ggccattcgg gaacccggca ggatcaccgg
    20761 caataaatcc gaggcgattg cccgtgccgg ttccggtatt cgtgccgacg ctcggcgcaa
    20821 tagccagatt caaacgcagg cgttcctgcg ggatggtgtt aggcggttgc agaggaaacg
    20881 ccgcgacact cgggcttcca gaggtattcg tttgggcatt gagtccgccc acgcccgtca
    20941 aaaagatgac tccgatgtcg ctgcgcggcg gcgcgggagc gtcgaaactc gggtacacgt
    21001 caaacagctt gcccggtgag ccagccgcgc ccggataacc gaacagcctg cccggttcgg
    21061 ggtcgagcac gaagttgagg aactgcgaat cgttacccgg cagagacgca ttgaatctgt
    21121 ctttcatgcc gagcgggatg atcaactcgt tgacgagcgg gttgccgagg cgcgaaacct
    21181 gaacaaagtt tcccgatatt gtggcgcggc cactgcgatc ttcagcaaag gcgatcatcg
    21241 ccggacggct ggatgcagag taaaccccga tgacggcgtt cggatcgtta ggtccggtcg
    21301 gagccgcccc ggttttagtc accgtggcga ccggcacttg catggcgatg gtgctgatgt
    21361 tgaagcccgc caacccgtct acgccgggcg tgttcgtgcg cggaatcaaa tgagccgagt
    21421 tgaacggtcg cagcgcgaag agatcgaagg ccgcgccgac gtcaatgaag aagctctctt
    21481 cgcgttgacc ggcaaacact cttgcatcgc cggacgcggt gctgatcgtt gccaccgccg
    21541 gctgcgcaag gtttgtctcg tagtccggcg tcgaacgcgg gccgacgttc acgggcggaa
    21601 cggcgaacgg gaagcccgca ggtgatccgg cagcggtagt cgtgggcgtg agcgtcgtgg
    21661 tcgtggtgtt cggcgcgttc ccccggatga cgttgatcga atagaactgc ttgatgttca
    21721 gatcggggtc gttgagcgac gtgaccggtc ccgtgttgta aagaaaagtg ttgggattgc
    21781 ccaccaccgt gcggaaacgg aaaacatatt gaatgtccgt gatcgcgtcg ccgttgttgt
    21841 ctattctcat cgcgtattcg acgttatcgt cgaagcggta gaagttcggg ccgccctgcg
    21901 gcaactggaa cggaatgaaa ttcatgatca ggttgacctt cgtggggtcg ttcggatcaa
    21961 cgaacgcata tacgtccgtg ccgtcaacca ccggatcaac cgaggtggat ggcgcttcgc
    22021 ggtggcttga tgcacggaca gcggtgctga atgttccggc ggcaagcatc gaaattatta
    22081 gcaggctaat atgagccgct cgcaagagtc ttcgcatagt gattcctctc cctgtgatag
    22141 tgcccgtctc aaactgtggg cggcgcgcgc cgggctagtt aaggcacacc gcataacttt
    22201 tgcgtcttaa gcaacggaaa tacttgtccc ttaaacgctc tgtaaatgtg aatgaagcgc
    22261 gtgagcggga ttcgtcgtcg aaaccgagaa tcatctctca gcgcacgagg catagctctt
    22321 tatcagtgtc gcaacgtttc aaatttgaaa ttcccgccgg taataattcg tcagattttg
    22381 cgagaaatat tttcttccgt attccacatt gatagtcttt agaatacggc gaagaaagac
    22441 ttcccgccgg taccggacgc agattaagaa tagggacggc gaagtataaa acggcgtgct
    22501 ttgcattact gtccgcagga agatgcaggc ttgtcgggga atctacggac ggggggagct
    22561 attgttgaca aggttatggg acaagctact gataaaatca tgccggtcgt cgaatttgca
    22621 caattgcttt tttaatcttg ttcgttgttc cggttctcat agcgagggag agtatcagga
    22681 gtgtcgagtt tcacagacaa tttgaccgtt cgcccgctca actcggaaga tgcttccgac
    22741 ctctccgcgt tgctgctggc tcaaactccg gcctatgtgc ggttctttac cccgttcaac
    22801 tttgatcttc cttccgttgc taccctactg gccgagcagg gtacggatgt ctttatgggt
    22861 atgtactggc aggatcgcct gatcggtttt ttcatgttgc ggggttggaa cagcggctat
    22921 aagattcctg cttacggcgt cttaattgac gaaaaatata gcggctacgg attgacgcgt
    22981 ctgtccctta aattggctaa aagcatttgt aagctgcgcc atgtgccgcg aattatgttg
    23041 aaggtcaacg ccgataatat aatcgccaaa agacttttcg aagagtccgg tttcactcaa
    23101 accggagttg acgctcaaac cggaaacctg atctatcact ttgactttag accccactaa
    23161 ccggattgcg cgaatcggat tttggttggg cgatgcttcc gcggagcatg gaggcatcgc
    23221 gccttacgct caacgcgtgc tcacgtctct tgtgagtaat gccgaacccg gctggcgcat
    23281 cgtcctgctg tgcgcgggcg ttctgcccgt tatcattaaa caactcgcaa ccggtcatcc
    23341 gggtttgtta gaaatcgagt tgctgcccga ccctccggcg gacgcgcagc cgcgacccct
    23401 cgataaaatc ggcgcgcgat tccgtcgtga taaaacacct caactatcct ggcgctatct
    23461 gcaagattgg ctcgcccgtc tcgaactcga cctgcttcat tttccgaccc agaccttgct
    23521 tcaccccgat atgcaaacgc cgtattgcct gacgatgtac ggcgtgcagg agctacccca
    23581 ccttgtcccc cccgtgcctc acatcatcac tatgcacgac gtgcaagagt tgcacttccc
    23641 ggaaaacttc acccccgcgc aaagggcgat ccgcgccgtg caatactgga aggcactcga
    23701 aaaagcgggc gtggtaattg tctctttcga ccacgtcaaa caagacctga tcaaatactt
    23761 cggactgccg gaagacaaaa ttcgcgtctg cccgattccc ttccgcgaca tctccttaca
    23821 ggagccgacg cctgccgccg cggagtctta tgcacggaag tacgaagcat ggacgccttt
    23881 cctgctctac ccggcgcaca cctggcggca caaaaatcat ttccggttgt tacaagccct
    23941 gcgagagttg agaaaggata gcgggcaaaa cttaaagctg atttgcacgg ggggcaccgc
    24001 acaccattac cacgcggagg tcttagcgca agttgacgag atgaaattga gcgatgccgt
    24061 tctcttctcc ggaatccttc cggaggacga actgcgttgg ctctacgagc aggcggcatt
    24121 agtgacgatt cccacgaaat acgaagcggg cagcttccct ctatacgagg cgatgctgct
    24181 cggtagcccg gtgatctgtt cgcatgtgac ttcactgccg gaaacaattg gtgatcgccg
    24241 cttcgtcttc gatccggatg atatcgaatc tttagcgagt cttatttttc ggatgctgac
    24301 tgacaatgtc ttccggcagg cgaacctcgc caacagtgcg acgcaggccg agcggctgcg
    24361 gagagttaac gcagccgaac acttctacga cgcctaccgt caactgctgg cgatcccatc
    24421 aggttcgact ctattgtttc gttaagagac taagcaggtc gcatcgaatt tgattgaaga
    24481 taaaagagac ttcaacgcaa gtattcttct atcaatcgag ggagggcttc aatgagcgcc
    24541 gcatcttttt gacgcgcgct ctggccgccc ccttcaaagt tggcgacgac gtaatcgaga
    24601 taggtcttct gaattttatc gttcccgaag catttaatgt tgaagacata atcggcgaga
    24661 attgggtaat cgagttcata ccgtccgatc acatcgaaga tcgctctttc ataaaagatc
    24721 gcctgatggc agatgttgta tgtctcaagc ctcgacttgt tgaactcgcc gccatagatg
    24781 aaagcctcat ccacccaata cacgttcccg taaatgaacg cgagttcccc atcaggcatc
    24841 aaccctgcga gatttctgag ggcgttggcg cgcaagatat cgcctgcccc gaggaaatag
    24901 atgtacttgc cgcgcgccat ctcgatgccc ttgttcatgg cgtcgtatat gccggtgtca
    24961 ggctcggaga cgaatttaat ctgcgcgttg aacctctcga tttcgctttg cgtgccgtcg
    25021 ttggaatcac cgtcaacgac gatgtactcg tataagccgt tgtcctgcga aaggacggag
    25081 ttgatcgttc gcacgacctt cggcccgcag ttgtaagtgg gggtgataat tgagaaaagt
    25141 ttctgctcca tgtttattcc gtagaagtgc ccgcgcgggg tttaatctcg taatggtaaa
    25201 tcaggttgcc gcttgttgcc tccacgccgg tctgaacaaa cccggtcttc ctcgcaacgc
    25261 ctttggcgga gatattgtca gggtgcattt tgatcatcag gcgcgacacg tttcgcagac
    25321 ggcaaatcac tttggctgtg tccagcgcag ccatctccaa accgtgtcca cggtattgct
    25381 cgtcaatcag gatgccgaaa gcggggactt catacccttc gttccacccg cgcaacataa
    25441 agaacccgac gatcccctcc cgccagaaca agcccataaa cacgtctcgc ccccggcgcg
    25501 cgagcgcgtc ggcaatcgca ggctcgtcgt agccgaacgg gtggaagaaa cgcgcataag
    25561 ccggtggctg tgcgcggagc cattgagaca gagcggaggc atcttcaacc cgcagcggac
    25621 gaaacatcag atttgcgatc tccattgctc tactctacca aaccaataaa ggctgttatc
    25681 tcgaacacgt tgattaacga aggcacgccc gcagggcgct cgtgatgtaa gtgatgtcgt
    25741 caggtgtcag taaagccgac gagggcaggt taattccgcg tgcggccaga ttggtggcga
    25801 tggggagcat cgagtcgccg tcgcgctggt aatacggagg gagatggtgc atcgggtgga
    25861 agaagggtct cgtttcgata ccttgctcca gcagccgggt cattatttgg tttcggtcac
    25921 actcttccgg gaggcagatg gagtagagcc agtagacgct cttcgcccgg atggactcat
    25981 gcggagttat gacagggagc gaccgcaacg cctcgtcgta cagcgcggca acttcgcgtt
    26041 ttctttcaat gaaccactcg atgcgctcta attgcgccag cccgatggcc gcctgaatat
    26101 tcgtcatgcg atagttatat ccgacaacgg gaaaccagta gcggcgcgcc gggtccatcc
    26161 cctgcccttt cagccggcga attttatcgg ctaacacttt gtcgtttgtg gtaatcatcc
    26221 cgccctcgcc cgtggtgatg attttgttgc cgaagaaact aaagacgccg acatcgccca
    26281 agcccccgac ccgtcgctct ttgtattcag cgccgtgcgc ttcggcggca tcttcgacga
    26341 tagacagatt atgcgcgctc gccagttcaa ggatcggatc gaggtcggct ggatgcccgt
    26401 agaggtgaac cggcatgatc gctttcgtcg cgggagaaat tttgcgggcg acatcctgcg
    26461 ggtcaagcgt ccaagttata gagtcaacat cggcaagcac cggcttcgcg ccgcaataag
    26521 tgacggcgtt ggccgaggct acatatgtca ggtcgggcac gatgatttca tcgccggggc
    26581 ctacgccgag cgcaaggagc gcgacgtgca atccggcagt tccgttcgcc accgagacgg
    26641 cgtgctcgac gccgcaaaag gccgcgaact ccctttcaaa gtcgtccaca tatttgccgc
    26701 tgccggaaat ccaccccgtg tcgaggcagt ccagaacgta tttcttctcg ttgccgtcca
    26761 gcactgtttc ggcgacggga atgaatcttc gtctcctcgt ctcaactctt tcagccacct
    26821 gctcctgcaa catcaaggcg ctcattatct ctccaaatct ctcccgcgag gcatcttccg
    26881 gcaagttcaa agcacggatt caaaggactc caagtactct cgcgcgacgt ggttcaacgt
    26941 gcgcgagtct ataaactttt tcgcctcggc ggtgcgttgc ccggactctt taagatttcc
    27001 gaagtgttta cagagcgcat ctgccaacga ctgcggcgag tacggatcga aggttaaggc
    27061 cagcctgtcg tccgtcccaa gcctttccac aaattgaggg atgttggaac aaatgatcgg
    27121 cttttcgtaa tacatcgcgt cgaagatcat ggaattgaac ccggcttcgc tgatcgaggc
    27181 tgtcaccacc gccgaagcat tttgataaag cgcggagatg tctccgtctc tcacataacc
    27241 caagctcaac aggtcgcgtc cgacttctaa cccgtgcagg ttgccgccaa gtaatatctc
    27301 cgtgagcggc ccggcgacaa cgagcgcgag attcgtctcc actcttcttt taaacagtgc
    27361 gaagccttcg acaatcgttt ccacgcgttt atgtttggca gcccagccca aagtaaacac
    27421 gtagtcagtc ttgatgccgt acctgccgag gacgcccggc gtttgcccgt atgaccctac
    27481 gctcgcgggc ggagtgaggt agatgcgctt cgtgcgcccg ggtggcaaat cgtaaattct
    27541 atgcgcctcg ttttgaatat aagttgaaga gaagatcacc aaatttgcga gagatgccca
    27601 ccttctaacc tcgctttgta aagaactcgt catctcgccc caagcgtccg tgtgctcgtg
    27661 cgcgaggtcg tgcagagtca cgacaagtgg cttcttggga tgccattcca catctcgcgg
    27721 cagcgtcagg taagggtaag gcaaatagat caagtcgaat gcgtgcgccc tgtcatccaa
    27781 ccacttcaaa ccaatccccg tgcctgaacg aaaggtttgc tgctcttcct cacgcagcgt
    27841 tttctcataa gcctcgaccg gcttgagcga cgataggcgt ttcgtgctgc tcagttttgt
    27901 accccgaaag ccttcataaa cgcggtgaag aaatttatct tgcacaccag agcgcgcacc
    27961 gacccgaatt cgctcgaatg tggtgttacg caggcggggg ggggcgagga aattgttgct
    28021 cgaatcgaaa tgcggccaca tcaaatgaaa ccgccaccgc gagcctgcct gtcgcgaaaa
    28081 ctcttccaac agtttgaggg taaacaggcg caccccgccg ccgctgacta tctggtcgaa
    28141 gatgccgacg cgaataatct tttctttatt gcccactgct ggattgaagt tgctttgaga
    28201 gcagatcggc aaccgtgcca cgcggcagac tcaaggtggc cgtgacgcgc ttgccattcg
    28261 cggacacgct cgcgccctga tagatgatct cctcgtcgcg cccgcgtttg acggaggctc
    28321 cgacttcaaa gagcagcgta ctggcctgcg cccactctgc ggctttcttc gccgacggta
    28381 gttcggaaga agcgctgagg gaaagcgtgt ctccgttcaa acggagggtg aagacgatgc
    28441 gccggacacc gtcgaggaat tccaatgccc cgctcgcact caaggcttct atgaactctt
    28501 tggctaattc tctctgcggc gcggcaatga ttttttggtt gatcccgatg ttgctcaact
    28561 tgccgctccg gctgcgctcg gcagtggctt ccatctctat cggagagttc agatcgatct
    28621 cgccgctctc aaccatgctt cggcctttgt tcaaaaggtc taagaaagcc ttcgtgctca
    28681 cacgcttcgg ctgtccgagt ttaacttcta cagtctcttc atccgtcccc acgtctttca
    28741 acggctgctg ttgttgcgtc gtcttcttct ctgtggattt tgacgctgac ttccgtgatg
    28801 gctcttgact taaggagata aagggcatcc ccagcgctaa catcattgtc agtaaatact
    28861 tcttcatttg aaaactacct ttgcttgaaa tttcgtcacc tcatggagtt cccggtacat
    28921 acatgattga catgacgaac gagaatcata tgcacatgta aaccaaagtt aaaaattcgg
    28981 gggtcggtga agtataaaac ggcgtgactc tgctttactg tccgcaggaa ggtgcaggct
    29041 tgtcggggaa tttacggaca gagagaggat tttccggtag ggtagaggct gtgcaaccta
    29101 tgcgcgtccg cgaacgcaga gttcaagact catccagcca tccctgaagc agggcgtagc
    29161 gcacgatttc ggcgcggctg tggaaattta gcttttccat caggcgcgcc ttgtatgtct
    29221 ccactgtcct gacgctgatg tcgagccttg tggcgatctc cttgttggag tagccgaggg
    29281 cgatgagacg caagacctcc gcttcgcgct cacttaactc tccctgcggt tgtccctctc
    29341 tggtggattg cttacggacg taactcccta ccacgtcgct cactatcgtc gggtcgaggt
    29401 atgtttctcc accggcgact gcgcggatgg cacggatcaa ttcgtgcgga gccgcccgct
    29461 tgagtacgta accggacgcg cccgccttga gcaactgatg caggtagcct tgatcgttgt
    29521 ggacggtgag caccaacacc ttcgtctgcg gacagtcgcg cttgagcaac tcggtggctt
    29581 tggctccgtt cagttcgggc atggagatgt ccatcacgac cacatccggt tggagagctt
    29641 tgacctcgcg ccaggccgtc ctgccgttgt ccgtctcgcc gacgacgatc atgtcaggtt
    29701 gagcttcgat cagagacttc aacccttcac ggatgatcgc atgatcgtcg gctaagaaga
    29761 tacgcagttt gctcaaaacg atttaccccc gttctcatca agcgccagag gaatacgcac
    29821 gaaaatggtc gtacccgtgc cgagagagga ttcgatgttc agtgtgcctc cgacaagggc
    29881 tgtgcgttcc tccatcccca gcaaacccat tctgcgttga ccgacgctcg ctgccgacaa
    29941 accttcgaca tcaaatccac agccattgtc ttcaataatg gtgaacacgt gattgccccg
    30001 ccgctccaga atgagactaa cgtgggtggc gcgcgcatgt ttgatgatgt tgttgagcgc
    30061 ctcttgggcg atccggtata tggcggtttc gatctcagag gcgaggcgtt cgtctgcaag
    30121 gctgacgccg tagaagtcaa cctgcaaccc gtaacgcttc gaccatttcc cgatataatt
    30181 tcgaagcgcg gcgtgtaaac ccacgtcgtc caactccgtc ggcctgagtt tccaggcgag
    30241 agtatcaact tgcagtgaga gatgttgagc gacattctct aactgtgcga atttggcagc
    30301 ggtcgccggc tcagaacggg aaaagtcttt caaagagttg accagcaata cgatcgccac
    30361 gatatgctgc cccatctggt cgtggagttc gcgggagatg cggcgacgct catcctcctg
    30421 cgcggtgacg agttgccgca ataactggcg gcgcgcttct tccgactcct tgcgctccgt
    30481 gatgttgagc gtcactccaa tcatccgtgt aggttgcccg tctgccccgt aggagccgcg
    30541 accgcgcgta atgatccagt gcgcgctgcc gtcaggccac aggttgcgat actccgcttc
    30601 gtaatcagta tgtttttcta aagcctgttc cacggccccg cccacgcgcg cgtggtcgtc
    30661 cgggtgaacg gctgcaaaga gagtgtcata ggagaattca gcttcgggcg gaagaccgaa
    30721 atgcgccttg cagatgtccg agcaggagag cgtgcccgtc tcaaggtcta attcccacaa
    30781 accgagtttc ccggtctgga cggcgatctt caaacgctgc tcgttatcgc gcagcctctc
    30841 ttccgtgtgc gtgcgctctt cgatttccgc ttgcagagtt tcgttgatct gcgccaattc
    30901 ggctgtgcgc tcggcaactc ttcgctccaa ttgatcgtgc gcctgccgga gcgcctcttc
    30961 cgtttgcttg cgctccgtga tgtcgcggac catcgtggtg atgtaggtgc gtcctgcgac
    31021 agttgaagtt gagacggacg cttcgacatc aacgagcgtc ccatcctttc gctttccttt
    31081 aaattcgtaa cctgatatga cccgttcccc gctcccgcga cggctgccga atttcagcat
    31141 gcgttccacg tcctctgtcg agatgatgtt ggagacgtgc tgaccgatca actcttccgg
    31201 ggcatcgtag ccgaacagtt gcgcgtagga tttattgacg taaacgatcc gctcatcatc
    31261 ctcaacgaga atgccgtcgc gcgatgcggc gaacattatt cgcaaacgct cctcgctctc
    31321 acgcaaagcc tcttcggcgc gcttgcgctc ggtgatgtcc aacacgaccg caatggagga
    31381 gtgcggttta cctgcacggt cgcggatggc cgacacgccg aggttgaccc atacaaccga
    31441 accattctta tggatgtaac gcttttcgat ttcatagggt atgccttcgg caatcattcg
    31501 ctcgcgcagt gcgacgttaa gcggcatgtc gtccgggtgc gtgatgtctc gaatgcgcat
    31561 ttcgagcagt tcggctgccg ggtagcccgt gatgtcgcag tatttttgat tgacgattgt
    31621 gaagttgtcg ttcaaatcgg cctgccccac gcccaccatt gcctgatcga tgatggcgcg
    31681 gtaacgctct tcggattcgc gcaatgcttc ctccgcacgc ttgcgctcgg taatgtcggt
    31741 ggaaacgccg acgatgccga ttaacgctcc ttcgtcgttg taaatcggcg tgtcggtgat
    31801 gaaggcgcga aagtttgtgc cgtcccgacg ccgcaccata aactcgcccg accagctttg
    31861 acccctgtgc aggcgcgaca tgatttcaac agcctgatct tgtgcggctt gtgggagaac
    31921 gatctcaagt atgctgcgtc ccgttatttc agaggcaggc cagccgtaga gcttttcggc
    31981 gaagcggttc caatagatga tcctgccgct caaatcagtg gcgattacgg cctgctcgac
    32041 cgtgttcagc aagttagact gaaagcgtat cgcttcttct gactgcttgc gctttgtgat
    32101 gtcgcgaata atggcttgca acactccgtc aggaagcatc cttccgctaa tctccacagg
    32161 gagcagcgtg ccgtctttgc agcgcactac ccgctcgctg atgactgtct cccccgcacg
    32221 taagttgccg aggcggagcg gggctgcttt caggtcttcg acgggaatca gatcacggac
    32281 gttgagccgc agcagttcat ccctagtgta accgagcatc tggcatagtt tcgtgttggc
    32341 atcaatgaaa tttccttcaa agtcgtaggt gtggatgccg tcggaagcct gctccatcag
    32401 cgtccggtat ctgctttcac tttctcgtag cgaaagctcc gtctttttcc ttgccgtgat
    32461 gtcttctgcc gtcccggaat ggccgacgac gacgccctct ttcgagaaca tcggtgcggt
    32521 gcggatgtga acccaacggg tgattcccgc cggtgtctga aagcgcaatt ccccggagta
    32581 ctcccgacct tcatgcgcat cggcagacca acccgcaaac gattccgcgc gatcttcagg
    32641 gtggacaaac cgcccccact cttcgcccga acacttttcg gagggaaatc cacagatcaa
    32701 acggaaacgc gcgttagcgt aaacacagcg gccttcggcg tcggactgga agatgcccac
    32761 aggagaacgg tcacacaagg cgctaaaaca ttcctcgcgt tccaccggta ttatgtccgt
    32821 ccgctcttgt attgtttcgc ccgggttaat actgcacctc aatctgtaac tctgttctcc
    32881 ttcggagagc gtaatcagga attattagga aactataacg atctaaaggt gaatctgttt
    32941 tgccctgata cacacctgat cagaattaac cgacgctgct ttcacgatgt tttctcaatt
    33001 tccaagcggc ggtgtaagtt caaccacgca aaggtacgct cgaccaccca ccgacgcgcc
    33061 agcacgacga agccaagctg ttcttctcgg cgcaagacca cttgtaagac gaagcgaaag
    33121 tgcgctgtga cccacagagc gggcgatggc ccgcgatagc cgccatccac ccggatgcga
    33181 cgcagctttt tacacgagcc gcttctgcgc gccaagagta gacgcgcgcc atcgcgctct
    33241 tggactgagg cggcagtcac cgcgacgctc agcaataagc caagcgtgtc gaccaagagg
    33301 tgccgtttgc gtcccttgat gtttttcgcc gcgtcaaagc cgcgcactcc cggcacttgc
    33361 gtcgtcttga cgctctggct gtcgagacat ccggcggtcg gatgcttgtg gcgccccgcc
    33421 gtgcgccgga cttcggcacg taagcggtca tggagtcgtt gccactcatt ctgtttgcgc
    33481 cacaggcgga agtagtaata gacgctctgc cacttgggaa aattacatgg caacatgcgc
    33541 cattgaatgc ctccgaccac gacatagaga atggcattga cgacctagcg catctccagt
    33601 tcacgcggcc ggcgtggtcg tttggctttg ggcagcaaat ctttgatatg ctcccactgg
    33661 ctgtcagtca gatcagaagg gtagtttttg ttaggcataa ctcaaacata cctaactgaa
    33721 ctggctggca gtcaattttc aaacagcttc taatcgctgt ccttacaatc tttaaaaatg
    33781 cgcctccaga atttgtttca cggaaagttt cactcccatt aaatgcgcgt tagcaagaat
    33841 taaaatacgt tttttgcatc ggcacaggac atttcgcata atgacaaacg ttatagatat
    33901 tatgagaaat aaaatattat ccgtaactct aatgattttg gcggtgttta ccgccgtttc
    33961 ggcgcagacg ctggacaaag ctaaactcga ccagtttttt gaccggctcg ccgaaaagaa
    34021 caaggcgatg gggagcctga cggttgcccg tgacggcaac attctctaca cgcgcgccat
    34081 cggctacagc cagatcaacg gcagcgagag aaagcctgcg accacggcga ccagataccg
    34141 ggtgggctca attaccaaaa tgttcaccgc cgcgatgatc tttcagctcg tcgaggaagg
    34201 aaaattgaag ctgactgaca ccctcgacaa attcttcccg cagattccga acgccaagaa
    34261 aattactatc gcgcatattc tggcgcaccg cagcggaatc cacgacttta tcaaagaacc
    34321 cgattttcgc tcctggtcga tgaacccgag aacgaaagac gagacgctcg ccttcattgc
    34381 caaggggacg cctgatttcg agccgggcga aaaacgcagt tacagcaatg ccggatatgt
    34441 gcttttgggt tttgtcgtcg aaaagttagc cggcaaacct tatcaggacg cattaaagaa
    34501 aagaatcacc ggaaagctcg ggctgaagga tacctatgcc ggcacgggga aaaccgacgt
    34561 cagcaaaaag gaaagctttt cctacagtta cgccggagac tggaaacagc acgaagaaat
    34621 ggatttgagc attccgggcg gcgcggctgc gatcatttcg acgccgagcg atctcgtcaa
    34681 attcattcag gcgctctttg acgggaagct catttcgcag gagaacgtca atcagatgaa
    34741 aaatggaatg ggaatgagtg aacacaagct cggcgacaaa acaatctatg ggcatggagg
    34801 gggcacggac ggctttaggt cgacggtact ttacctgccg gaagaaaaac tagccgtcgc
    34861 ctataccgcg aatgcaaaag tttactccct ggaaagcatc ttcagcggca ttttcgacat
    34921 ttactacaat aaaccgtttg agattccgac cttttaatca agcgccgtca gcccggaaat
    34981 tttggaaaaa tacctcggag tatattcgag cccgggaact cccgggaaat ttacgatcac
    35041 cagagaaggt gcgacgcttc acgcgcagcc gaccgggcag tcctctttcc cgctcgaagc
    35101 caccgcgcag gataaattta aattcgatcc tgtgggggta gttatggaat tcgacgccga
    35161 gaaaaatcag atgatcttaa aacggagcgg ccgggaaacc gttttcacga aagaacatta
    35221 accgtcatta aaattgtagg ggcaggtctg aacctatctt ggattcagaa ttggtgttta
    35281 attctttaga tatagtattc aatagataag cagcccaacg ctggcgataa tctgcccgcc
    35341 gcgcacagcc tccgatctga tttatatatt ctacttgaga gacaagctat cggcgggtca
    35401 gcttcagcgc cttg
//
LOCUS       DASWNS010000156        12877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_500022, whole genome shotgun sequence.
ACCESSION   DASWNS010000156 DASWNS010000000
VERSION     DASWNS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500022"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VGB76_05315"
     CDS             complement(<1..886)
                     /locus_tag="VGB76_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0098355.1"
                     /translation="MSTRQEANTFIITREDRSFDPVTLVSEGLAIGRLPDCELNLNHP
                     TVSRLHAGVRKIDGRFYIFHLSPSNSTTLNGKLVEEKAALADGDVVQIGPFFLFIDFN
                     ENALAIRVRYQTAVRVGDAELPTGSVQAMPGAPTTADVHPRSASSADAEGAETDEGAL
                     DVFWEKRKREAGKVARPSPLRPRPPAAKLGKARFNWKPTRDLARPWPFSIFTWGLVVV
                     GLLSIVAAFSYTSAFSPAPLSNAHARQNLSMPLAIAREPNANSCTTCHSLRTSMETNC
                     ASCHQTQAFAATVTPPHVA"
     gene            complement(955..3483)
                     /locus_tag="VGB76_05320"
     CDS             complement(955..3483)
                     /locus_tag="VGB76_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY0098356.1"
                     /translation="MPKEITKHKTILDAISKVDIISELVERHDDHFTYELDLEVVAYG
                     RNYNGKKVGPYVRLFEFEPGEEIIRQGDWGSNTFYITVAGMCDAYVNDENGTPKKVGE
                     IPEGVSFGEMAILAGVPRNATVKASPAKPATVLEVTRPALRLLRKLPKFGQNLDVTYR
                     KHGLGRTLVDVQQATGNAFDADLLKQLGAAARFMVYGKNHILHREGETIDRIVFIKNG
                     WVRCVSGLEFNPAMSNLAAALDEKSGLDFLGAGNCLGLEGVREDRSWSYTATILSRTE
                     VLEISISHLRANPKLRDAVTTKFARFSVADDKIEAVAEAAALQLGATEKEISTGIVDG
                     TNLLVMDMDLCVRCGNCSLACHKVHGQSRLLRRGIHIERPKKPDSASSQHLLSPSVCL
                     HCQDPECLTGCPTGAIGRFSNGQIDIDPKTCIGCGDCATQCPYNAISMIPRKPVAPAP
                     VTWQGRVRKWFSLAPPEPPPEVTDTENLLAVKCNLCNNTGLNPKGAKTHAYSCQENCP
                     TGALVRVNPREYFTEVKNRIGIVFQDQTHAIGRNIHKRDIPARLWHAGGILSTILFTA
                     LTIWAIFRYGLDGSIGGTWLTVRWLTGLVGLVGIAIVMAYPGRKQIYRRRAGALRYWM
                     LAHVYAGIVAGLLLLLHGGRSTGGLLTSLLMISFDLVILSGLFGIVCYIVVPRIMTSI
                     EGEPLLIEDLRMRRDELRESLAHIGTQSTPAVRELIRRKVRPRYLSLGYLLRQFVRRE
                     DLTSLHSMAREDFKTELEPYKKRSEELSGTFLFGADDPSQYRPEGRLLLDAIESTVTL
                     RRVDSLIYLHQLLKLWLAPHVVATSLMLVLMLVHIIQVIFFRVH"
     gene            3864..4520
                     /locus_tag="VGB76_05325"
     CDS             3864..4520
                     /locus_tag="VGB76_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0098357.1"
                     /translation="MSAISSIDELLLPARATVARQSSAVAEPLSDHSLLEAARTGDEA
                     AFAELVRRYRNQITNYVYRLTNDYESAVDLAQETFMRVFRAADRYQATYAFSTYIYRI
                     ATNLAISELRQRKRRRLVSLTGFFQSQDQAGEPCELDPADKRPLQDAAMVDDERRAAV
                     ARAIATLPEKYRAPLVLRDIEERSYEEIGRILEMSEGTVKSRISRARTFLRDKLQDYL
                     "
     gene            4542..5375
                     /locus_tag="VGB76_05330"
     CDS             4542..5375
                     /locus_tag="VGB76_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098358.1"
                     /translation="MNCQKCKIEIEERNLRRERLSDAATAHLSACASCRVFGEERLAL
                     SRLVGGLEKVSAPADFDFRMRARMAAEANAGNATRARWFNFSPAALSWPLAACFALVV
                     SAAFYFQQQQPASVTATPDEAARTAVVIEPPTTSRASSGGATAQSAPVKNVETARVAV
                     APSLKNPTVVRRRALPARGGEQTTVEAAQIRVRLEESNSVGLLGSPLRHAVNGAAQGE
                     GASIPVQVSTQERPLKVLLQETGGARTISVESVSFGSRDVIGRPATISKASLSTNQGV
                     W"
     gene            5402..7387
                     /locus_tag="VGB76_05335"
     CDS             5402..7387
                     /locus_tag="VGB76_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0098359.1"
                     /translation="MRNLFAACGGRQDGRTLARLAVCGALLYACGAQPVYAQSPQGRP
                     SPPRRAPMPPVPTTQTKSGVAAPAKFQAAAPQVIAVVHRMSGWKLRTLLTPADASSAS
                     NFDENFVRTNIVAGFILPDGRSVIARLPQAEAEMLNLSTAFRTPGSPAGATTDDAELL
                     LVRPDGVQFRARFIGLDASTGLSLLESDKPLLAPAAEPMPNIPPVVGQRVRVIAPVRA
                     ATPAAAPAAAPIAPAAATPAISTARPEDAPVGDEGVIYMNMSEEAGQLREVRRSPSGR
                     AMEMTLEVDRVSPEWAGGVALSETGTLIGIIEESDARATRLMSAETVRGAARRVRARR
                     ASVPQPWLGARGDAVAFAPLEQFLARGWRPEEARLLVSRQRGVLLTSVAPGTPAAHAG
                     LRPGDVISRIGEHDVRSIEDMSSMIKELGGNTLANFTVLRAESAPLKLAVRLSESPSP
                     GLDTARAELRALQSELRATQFAINSTQKDLRRLESELSRIESERERIGATDAFVSQKM
                     LEEWKRTQARHRAMLEQTRKISTDYEQVRARMTEAEARYRMAVESYSGLAVRPLLSFG
                     LEAVRISPAATKLLGTQATQQQQSGLLVISVRPGSAAARSRIQTGDIIESVNGQKFSE
                     TNWNFNFSDEFDAVLSLGILRSGEKLSFDLSRQNSPR"
     gene            7576..8931
                     /locus_tag="VGB76_05340"
     CDS             7576..8931
                     /locus_tag="VGB76_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098360.1"
                     /translation="MLHEAIERYHGLLADDDLAEASRKMLDEGLERAKLIFGGRRLSP
                     YLRPHFVTETDFARIQTVCEIVWSAIQKVKDAAVGNDELLDELGITEIERDLVSIDPG
                     YEAVSPTARLDSFLTEEAYSFVELNGESPAGIAYVDAAYKIFSELPVMQRFAEHYNVR
                     PLYGRHFMLEVLLRAYTEYLGRTPERKPLIAIVDLKDLPTQKEFELFQEYFEGEGYRS
                     IVCSPQELEFSGGRLRSGADEIDIVYKRLLVNEYIPIMEEFPALLDAYRAGAICMVNS
                     FRSKLIHKKALFAVLTNERYAELFTAEERAAIRAHVPWTRKVRNETTDHYGEQINLLE
                     FAANSRDRLVLKPNDDYGGHGIYIGWNTDEIGWDEALRHALSNGDYLLQERVSTARES
                     FPALQPDGTFEFAEQLVDLDPLLFFGKVGSAFTRLSFTELANVSSGGGMVPTFIISEK
                     T"
     gene            9068..9403
                     /locus_tag="VGB76_05345"
     CDS             9068..9403
                     /locus_tag="VGB76_05345"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0098361.1"
                     /translation="MSEEFDAESNLPVDDRRQFDRSRLIVDVFFDGADGTAVASTKDI
                     SPGGLYMNTQTKLPEGGLLTLRLPFPEGDVIVNAEVVYSNPGLGVGVRFHGLTEKDRA
                     IMQRALPES"
     gene            9403..9927
                     /locus_tag="VGB76_05350"
     CDS             9403..9927
                     /locus_tag="VGB76_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098362.1"
                     /translation="MVETQDAQLRWDELPETVRADLAHKLENLYGQEDDESAFDALAV
                     DKQQALLLFAVRLRALKLWPEIESIENVYGLGGVGLNIRARRSLTSTLAAHEKFSSRF
                     AAHGDTARGFFETGRTRAALHFLRMHGDAPRWSIHFDLHAPTANARSLFLHFWHEKWR
                     GETPDWRAIKAALV"
     gene            10089..11204
                     /locus_tag="VGB76_05355"
     CDS             10089..11204
                     /locus_tag="VGB76_05355"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEY0098363.1"
                     /translation="MFEKYTLGIEEEFQIVDPVTRELRSHVSEMLEEGKMLLGEKIKP
                     EMIQSMIEVGTGICHNIQEARTDITNLRSIVSSLARKQGLEVVAASTHPISQWREQKI
                     YDDPHYTLLIEELQMVARSLLIFGLHVHVGIIDRERQIHIMNAARYFLPHVLALTTSS
                     PFWLGINTGLKSYRSEVFKKFPRTDIPDHYDSYASFQRYIKLLLDTNCISNPRKIWWD
                     VRPHPVFETLEFRVCDIPTRVDDTIAIAALFQAIVAKLDKLIEKNLGFRLYRRALIQE
                     NKWRAVRWGLEGKMIDFGKQKEVPVRDLILELLDFVDDVLDDLGSRKEVEHIHTILER
                     GTSADEQLRVFGETNDLKAVVDRLITRTMENVPTIQI"
     gene            complement(11299..12228)
                     /locus_tag="VGB76_05360"
     CDS             complement(11299..12228)
                     /locus_tag="VGB76_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098364.1"
                     /translation="MMQEVKETITPPDQEEPLPFPFNQRTFCRFEPIEKRIEAIRVLV
                     IDDLLRDERDLSEMAVGEWGRRAKSHLQRERQIAGLAVGNIRNNISRLVREPETHVAH
                     VSEIAEAAAEFEPQAIVLSGTLRDFDYYHPSIVENFGRFIRTTKIPVLGICGGHQLVG
                     LGFGARVVTLDNMEQHERREGRPFEYQYRFIRITNSEDPIFEGVGDRDSGIWQDYTIE
                     ACILRVWQNHGLQLDRIPEGFKLLATAYFCRNQMMVKRTGGQLIYTVQFHLEKSFEDW
                     DKTRATRWEHPNESRDGRIIFENFLRQSLNHAA"
     gene            complement(12262..12801)
                     /locus_tag="VGB76_05365"
     CDS             complement(12262..12801)
                     /locus_tag="VGB76_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098365.1"
                     /translation="MSSATKRAGRSRAPDSLEALAAQIKETLGEVWLPRFYRERILPL
                     RTRAHALQATPATKPEQVNVQHTLLGVELKIGRRRMSCPDLATARYLATFARAGCANV
                     AVPYDISRISHLADELESSWQRMLLLVEHLAGARNKAYRTRLRNSLIADLRREIEEAG
                     AGTQIPQFNQNTKQRPPRV"
BASE COUNT         2515 a         3812 c         4047 g         2503 t
ORIGIN      
        1 cggcgacgtg cggcggcgtg acggtcgccg cgaaggcttg cgtctgatga cacgaggcgc
       61 agtttgtctc catgctcgtg cgcagcgaat ggcaggtcgt gcaggagttg gcgttaggtt
      121 cgcgtgcaat ggcaagcggc attgagagat tctggcgcgc gtgcgcgttg gataagggcg
      181 cgggcgagaa cgcggaggtg taactgaaag cggcgacgat ggagagcagc ccgacgacga
      241 ccagccccca ggtgaagatc gagaaaggcc acgggcgtgc gaggtcgcgc gtcggcttcc
      301 agttgaagcg agctttgccg agtttggccg cgggcgggcg cggtcgcagg ggcgaggggc
      361 gcgccacctt gcccgcctcg cgcttgcgct tctcccagaa aacgtcgagc gcgccttcat
      421 ccgtctccgc gccttcggcg tcggcggaag acgcgctccg tggatgcacg tcggcggtgg
      481 tcggcgcgcc gggcatcgcc tgcacgcttc cggtcgggag ttcggcgtcg ccgacgcgca
      541 cggcggtctg atacctgacg cgaatggcga gcgcgttttc gttgaaatct atgaagagga
      601 agaagggacc aatctgaacc acgtcgccat cggccagcgc ggccttctct tcgacgagtt
      661 tgccgttgag cgtggtggaa ttcgaggggc tgagatggaa gatgtagaaa cgcccgtcaa
      721 tttttctgac gcccgcgtgg aggcgcgaga cggtcggatg attgagattg agttcgcaat
      781 cggggagacg cccgattgcc agcccttcgg agacgagcgt gaccggatcg aaggaacgat
      841 cctcgcgcgt gatgatgaac gtgttggcct cttgccgtgt gctcactgga attgactccg
      901 ctggccgccc gacgcggcgg cagccctctg gatgtttggc gagacagatg ctccctagtg
      961 cactctgaaa aagatcactt gaatgatgtg cacgagcatc aacaccagca tcagcgacgt
     1021 ggcgaccaca tgcggcgcga gccacaactt gagcagttga tgcaggtaga tcagagaatc
     1081 aacccggcgc agcgtgacgg ttgattcgat ggcgtcgagc agcagacgcc cttcggggcg
     1141 atattgcgaa gggtcgtccg cgccgaagag aaacgtgccg ctcaattcct ccgaacgttt
     1201 cttgtaaggc tcaagctccg tcttgaaatc ttcgcgcgcc atcgaatgaa gcgaagttaa
     1261 atcttcgcgc cgcacgaact gtcgcagcaa gtaaccgaga gagagatagc gcgggcgcac
     1321 tttgcgccgg atgagttcgc gcacggcggg agtgctctgt gtgccgatat gagcgagcga
     1381 ctcgcgtagt tcgtcgcgcc tcatgcgcag gtcttcgatc aggagcggtt cgccttcgat
     1441 gctcgtcatg atgcgcggca cgacgatgta acagacgatg ccgaaaagcc ctgagaggat
     1501 gacgaggtcg aaagagatca tcaagagcga ggtgagcagg ccgcccgtgc tgcgcccgcc
     1561 gtgcaggagg agcaacagcc ctgcgacgat gcccgcatag acgtgtgcga gcatccagta
     1621 gcgcagcgcg cccgcgcgtc gccggtaaat ttgcttgcgc cccggatagg ccatgacgat
     1681 ggcgatgccg acgagtccga cgagtccggt cagccagcgc acggtgagcc aggtgccgcc
     1741 aatgctgccg tcgaggccgt agcggaagat cgcccagatg gtgagcgcgg tgaagaggat
     1801 ggtggagagg atgccgcccg cgtgccagag tcgcgccggg atgtcgcgct tgtgtatgtt
     1861 gcgcccgatg gcgtgcgtct ggtcttgaaa gacgatgccg atgcgattct taacttcggt
     1921 gaaatactcg cgcgggttaa cgcgcacgag cgcgcccgtc gggcaatttt cctgacacga
     1981 ataagcgtgc gtcttcgcgc ccttcggatt aagacccgtg ttgttacaga ggttgcattt
     2041 gacggcgagc aggttttccg tgtccgtgac ttccggcggc ggttcgggcg gcgcgaggct
     2101 gaaccacttg cgcacacgac cctgccatgt cacgggcgca ggagcgacgg gcttgcgcgg
     2161 gatcatcgag atcgcgttgt agggacattg cgtggcgcag tcgccgcagc cgatacaggt
     2221 cttcgggtca atatcgatct ggccgttgct gaaacgaccg atggcgcccg tggggcagcc
     2281 ggtcaggcat tcggggtcct ggcaatgcag gcagaccgaa ggcgagagta agtgctggct
     2341 cgatgcgctg tcgggcttct tcgggcgttc gatgtggatg ccgcgccgga gcaggcgcga
     2401 ctgcccgtga accttgtggc aggcgagcga gcagttgccg caacgcacgc acaagtccat
     2461 gtccatgacg agcaggttcg tgccgtctac gatgccggtg gaaatctctt tctcggtcgc
     2521 gccgagttgg agggcggcgg cctccgcgac ggcttcgatc ttgtcgtcgg ccacggagaa
     2581 tctggcaaac ttggttgtca cggcgtcgcg cagtttggga ttggcgcgca ggtgcgaaat
     2641 cgaaatttcg agcacctctg tgcgcgagag gatcgtcgcg gtgtatgacc agctacggtc
     2701 ttcgcggacg ccttcgagtc cgagacaatt gcccgcgccg agaaagtcga ggccggattt
     2761 ttcgtcgagc gcggcggcca gattcgacat cgcagggttg aactcaagac cgctcacgca
     2821 acgcacccac ccgtttttga tgaagacgat gcgatcaatc gtctcgcctt ctcgatggag
     2881 gatgtggttt ttgccgtaga ccatgaagcg cgcggcggcg ccgagttgtt tcaggaggtc
     2941 tgcgtcgaag gcgttgcccg tcgcctgttg cacatcgacg agcgtacgcc ccagcccgtg
     3001 tttgcgatag gtgacatcga ggttctggcc gaacttcggg agcttgcgga gcaggcgcag
     3061 ggctggacgc gtgacttcga gcacggtggc gggtttggcc ggggaagcct tgacggtggc
     3121 attgcgcggc acgcccgcca gaatcgccat ctcgccgaag ctgactcctt cgggaatctc
     3181 gcccaccttc ttcggcgtgc cgttttcgtc gttgacgtag gcatcgcaca tgcctgccac
     3241 ggtaatgtag aaagtgttgc tgccccaatc gccctgccgg atgatctcct cgcccggttc
     3301 aaactcgaag aggcgcacgt aagggccgac ttttttgccg ttatagttgc gcccgtaggc
     3361 caccacttcc agatcgagtt cgtaggtgaa gtggtcgtcg tggcgttcga ccagttccga
     3421 aatgatgtca actttactga tggcgtcgag gatagtttta tgcttagtaa tttccttggg
     3481 catagagaag gatgattcca gattttaggt agagcgaata gatcggaagc gacagcgtat
     3541 aagcagatgc cttcgaaagt tgcgaaaggc tttgattgct gaacggcggg cattatagca
     3601 gagtccgccg tgtgtggtga gactttaata aaagcgtgcg catgaagaac cgcagacggc
     3661 gtgtggactg aaaattcgac acggttttct ggaacttcga gggcggtgcg gcgtgtacct
     3721 gaacgggcga ccgtgcggtt ggaagtgtat aatactgtcc gcgcaccggg tggcgcgcat
     3781 accctgccgg gggcgcggcg aaaagcgaaa actccccgtc ggacgccctg aagtatgagt
     3841 cggagagcct acctgtccta agcatgagtg cgattagttc catagacgaa ctgctgctcc
     3901 ccgcgcgggc gaccgtcgcc cggcagagca gcgccgtggc cgaaccgctc tccgatcact
     3961 ccctgctgga ggcggcgcgc acgggcgacg aggcggcctt tgccgaactc gtcaggcgtt
     4021 atcgcaacca gatcacaaat tacgtctatc gcctgacgaa cgattacgag tcggcggttg
     4081 atctggcgca ggagactttc atgcgcgtct ttcgcgcagc ggatcgttat caagcgacct
     4141 atgctttctc gacctacatc tatcgcatcg caaccaatct cgccatcagc gagttgcgcc
     4201 agagaaaacg acgccgcctc gtctccctga caggcttctt ccagtcgcag gatcaggcgg
     4261 gcgagccgtg tgagttagac cctgcggaca agcggcctct gcaagacgcc gcgatggttg
     4321 acgacgaacg ccgcgcggcg gttgcgcgcg ccattgcgac gctgccggag aagtatcgcg
     4381 cgccgctcgt cctgcgcgac atcgaagagc gcagctatga agagatcggg cgcatccttg
     4441 agatgagcga gggaacggtg aaatcgcgca tcagccgcgc acgcacattt ttacgcgata
     4501 agttgcagga ctatttatag tcggggtggt gtgaaaaaaa catgaattgt cagaagtgca
     4561 aaatcgagat cgaggagcga aacctgcggc gcgaacgctt gagcgacgcg gcgacggcgc
     4621 atctatcggc gtgcgcgagt tgccgcgtct tcggtgaaga gcgtctcgcg ctcagtcgtc
     4681 tggtcggcgg gctggagaaa gtctccgccc cggctgattt cgacttccgc atgcgcgcac
     4741 gcatggccgc cgaggcaaac gccgggaacg cgacgcgcgc acgctggttt aatttctccc
     4801 ccgcagccct ctcctggcct ctggccgcct gcttcgcgct cgtcgtctcc gccgcgttct
     4861 actttcaaca acagcaaccg gcgagcgtga cagctacgcc cgacgaagcg gcgcgaacag
     4921 ccgtcgtcat cgagccgcca acgacttcgc gggcttcgag cggcggagca actgctcaaa
     4981 gcgcgccggt caagaacgtc gaaactgcgc gcgttgccgt tgcgccctct ttgaagaacc
     5041 cgaccgtcgt gcgccgtcgc gcattgcccg cgcgtggcgg cgagcaaacg acggtcgagg
     5101 cggcgcaaat tcgtgtgcga cttgaagaat cgaattccgt gggcttgttg ggttcgccct
     5161 tgaggcatgc cgtgaacggc gcggcgcagg gcgagggcgc gtcgattccc gtgcaggtca
     5221 gcacgcagga acggcctctg aaagttctac tgcaagagac gggcggcgca cgcacaatct
     5281 cggtcgagtc cgtctccttc ggctcgcgcg atgtcatcgg tcgcccggcg acgatctcca
     5341 aagcctctct ttcgacgaat caaggtgtgt ggtagtggca ctgaaagttt taaaacagag
     5401 aatgcgtaac ttgttcgccg cgtgtggcgg gcgacaggac gggcgcacgc tcgcgcggct
     5461 cgcggtctgc ggcgcgctcc tctacgcttg cggcgcgcag ccagtgtacg cgcagtcgcc
     5521 gcagggcagg ccgtcgccgc cgcgtcgcgc gccgatgcct ccggttccta cgacgcagac
     5581 gaaatccggt gtcgccgctc cggcgaaatt tcaagccgcc gcgccgcaag tgatcgccgt
     5641 cgtgcatcgc atgagcgggt ggaaattgcg cacgctgctg acgcctgccg acgcctcttc
     5701 cgcttccaac ttcgacgaga acttcgtccg caccaatatc gtcgccggtt ttatcctgcc
     5761 ggacgggcgc tccgtcatcg cacgcctgcc gcaagccgaa gccgagatgc tgaatctctc
     5821 gaccgccttt cgcactcccg gatcgcctgc gggcgcaacg acggatgatg ccgagttgct
     5881 gctggtgcgt ccggacggcg tgcagttcag agcgcgcttc atcggactcg acgccagcac
     5941 cggcctctcg ctgctcgaat cggacaagcc gctgcttgcg cccgctgcgg agccgatgcc
     6001 gaacatcccg cccgtcgtcg gccagcgcgt gcgcgtgatt gcgcccgtgc gtgccgccac
     6061 gcccgccgcc gcgcccgctg ccgctcctat tgcgcccgct gccgccacgc ccgcgatctc
     6121 tacggcgcga ccggaggatg cgcccgtggg cgatgaaggc gtcatctaca tgaacatgag
     6181 cgaggaagcg gggcagttgc gcgaggtgcg acgctcgcct tcgggcaggg ctatggagat
     6241 gactctggaa gtagaccgcg tctcgcccga atgggcgggc ggcgtggcct tgagtgagac
     6301 gggaactctc atcggcatca tcgaagagag cgatgcgcgt gcgacgcgcc tgatgtctgc
     6361 cgaaacggtg cgcggcgcgg cgcgtcgcgt gcgtgcacgg cgcgcgagcg tgccgcagcc
     6421 gtggcttggc gcacgcggcg atgccgtcgc cttcgcgccg ctcgaacagt ttctcgcgcg
     6481 cgggtggcgt ccggaagaag cgcgcctgct tgtgagcagg cagcgcggcg tgttgctgac
     6541 ctctgtggct ccgggcacgc ccgccgcgca cgcgggtctg cgtcctggcg atgtcatctc
     6601 gcgcattggc gaacatgatg tgcgcagcat cgaagacatg tcctcgatga tcaaagaact
     6661 cggcggcaac acgctcgcca acttcacggt gcttcgcgcc gagagcgcgc cgctgaagct
     6721 ggccgtgcgt ctgagcgaat cgccgagtcc ggggctggac accgcgcggg cggaactgcg
     6781 cgccttgcag tccgaactac gcgccacgca atttgcgatc aactccacgc agaaagacct
     6841 gcgccgcctt gagtcggaac tgagccggat cgagagtgaa cgcgaacgca tcggggcgac
     6901 ggatgcgttc gtgtcacaga agatgctgga agagtggaag cgcacgcagg cgcggcatcg
     6961 cgcgatgttg gaacagacgc ggaagatttc caccgactat gaacaggtgc gcgcacgcat
     7021 gacggaggcc gaggcgcgtt atcgcatggc ggtcgaatcc tacagcgggc tggccgtcag
     7081 acctctactc tcgttcggac ttgaggccgt gcggatctct cccgcggcga cgaaattgtt
     7141 gggcacgcag gcgacgcagc aacagcagtc cggcctgctc gtcatctctg tgcgtccggg
     7201 cagcgcggcg gcgcgaagcc gtatccagac cggcgacatc atcgagtcgg tcaacggcca
     7261 aaagttctcc gagacgaact ggaatttcaa cttctcggac gagttcgacg cagtcctctc
     7321 gctcggcatc cttcgctccg gcgaaaaact ttcgttcgac ctctcacgcc agaacagccc
     7381 acgctaagca cgcagacgac ggaagggcgc aggccgaaag tgaagcagtg gacgcccgcg
     7441 cgccatccgc ttcgcctgcg ccctgcgccc tgcgcctttc ccctttttcc ttttggcttc
     7501 ccggttgtga tactctagcc gcacactggt tctagttctc caaatcgctt ttgcactaat
     7561 ttccacggga gcggcatgct acacgaagca attgaacgct atcatgggtt actggcggac
     7621 gacgatctgg ccgaagcatc gcgcaagatg ctcgacgaag gcttggagcg cgccaaactg
     7681 atcttcggcg ggcgtcgcct ctcgccttac ctgcgtccac acttcgtcac cgaaacagac
     7741 ttcgcacgca tccagacggt ctgcgagatc gtctggagcg cgattcagaa ggtgaaggat
     7801 gcggcggtcg gcaacgacga actgctggac gaactcggca tcacggagat cgaacgcgac
     7861 ctcgtcagca ttgatcccgg ctacgaagcc gtctcgccca ccgcgcggct cgattcgttt
     7921 ctcaccgaag aggcttacag cttcgttgaa ctaaacggcg aaagccccgc cgggatcgct
     7981 tacgtcgatg ccgcttacaa aatcttctcc gaactgcccg tcatgcaacg cttcgcagag
     8041 cactacaacg tgcgcccgct ctacggtcgc cacttcatgc tcgaagtgct gctgcgcgcc
     8101 tacacggaat acttgggtcg cacgccggag cgcaagccgc tcatcgccat cgtcgatctg
     8161 aaagatttgc cgacgcagaa ggagttcgaa ctgtttcagg aatacttcga gggcgaaggc
     8221 tacaggtcaa tcgtctgttc gccgcaggag ttggagttca gcggcgggcg tctgcgcagc
     8281 ggcgcggatg agattgatat tgtttacaaa cgccttctgg tcaacgagta cattccgatc
     8341 atggaggagt ttcccgcgct cttagacgct tacagggcgg gcgcgatctg catggtcaac
     8401 agttttcgct ccaagttgat ccataaaaaa gctctcttcg cagtgcttac caacgaacgc
     8461 tacgcggagc ttttcacggc ggaagaacgc gccgccatcc gcgcccacgt gccctggacg
     8521 cgcaaggtgc gcaacgagac gaccgaccac tacggcgaac aaattaacct gctggagttt
     8581 gccgccaact cgcgcgaccg tctcgtcctc aagccgaacg acgactacgg cggccacggc
     8641 atctacatcg gttggaacac ggacgagatc ggctgggatg aagccctgcg ccacgcgctc
     8701 tcaaacggcg actatctctt gcaggaacgc gtgagcacgg cgcgcgaatc gtttccggct
     8761 ctgcaaccgg acggcacgtt tgagtttgcc gaacaactcg tagacctcga cccgctcctg
     8821 ttcttcggca aggtcggctc ggccttcacg cggctttcat tcaccgaact cgccaacgtc
     8881 tcttcgggcg gcggcatggt tccgacgttt atcatcagcg aaaaaacctg agctgcaagt
     8941 cacgagcgat tgttgcgcgc ggcggcggtg gtgcgcgatt gaaaaacctg tgacgcgttt
     9001 tgtcggcttc gagtaagatg gagcagtagt tcgtcaaact gaatcatccg gcacaggaag
     9061 tgtaaacatg agcgaagagt ttgatgcaga gtcgaatttg cctgtggatg atcgtcggca
     9121 gttcgaccgt tcacgcttga tcgtcgatgt ctttttcgac ggcgcggacg gcaccgccgt
     9181 cgccagcacc aaagacatca gccccggcgg cctctacatg aacacgcaga cgaagctgcc
     9241 cgaaggcggg ctgctcacgc tcagactccc gtttccggag ggcgacgtga tcgtcaacgc
     9301 ggaagtggtt tacagcaatc ccggcctcgg cgtgggcgtg cgctttcacg ggctgacgga
     9361 aaaagatcgc gccatcatgc agcgcgctct gcccgaaagt taatggttga gacgcaggac
     9421 gcgcaacttc gttgggacga actgccggag acggtgcgcg ccgatttagc acacaaactt
     9481 gaaaacttgt acgggcagga agacgacgag agcgcgtttg atgctctggc ggtcgataag
     9541 caacaggcgt tgttgctctt cgccgttcgg ctgcgagcgt tgaagttgtg gccggagatt
     9601 gaaagcatcg aaaacgtcta cggcctcggc ggcgtcggcc tcaatattcg agcacggcgc
     9661 agcctgactt cgacgctcgc cgcgcacgag aaattctctt cgcgcttcgc cgcgcacggc
     9721 gacaccgccc ggggcttttt cgagactggc cgcacacgcg ccgccctcca ttttttgcgt
     9781 atgcacggcg acgcgccgcg ctggtcgatt catttcgacc tgcacgcccc gacggcgaac
     9841 gcgcgcagtc tgtttctaca cttctggcac gaaaaatggc gcggcgagac gccggattgg
     9901 cgcgcgatca aagccgcgct ggtttgaatc gcatgcgccg gatgctgctg tttatcctgt
     9961 gttcgtgacg atgccgtcag ggattgtctt tgcggcgacg cgcgggtgtt tgccgtaagc
    10021 gattgaagat gtcggtcttc cccctttagc tcaacacatg tcaggtcttg caaggagagc
    10081 gaaaacttat gttcgagaag tacacgcttg gcattgaaga agaatttcaa atcgttgacc
    10141 cggtgacgcg ggagttgcgt tcgcacgtct ccgagatgct ggaggaaggg aagatgctcc
    10201 tcggcgagaa gatcaagccg gagatgatcc aatccatgat cgaagtcggc acgggcatct
    10261 gccacaacat tcaggaggcg cgcaccgaca tcaccaacct ccgctccatc gtctcttcgc
    10321 tcgcacgcaa gcagggactc gaagtggtcg cggcctccac gcatccgatc tcgcaatggc
    10381 gcgaacagaa gatttacgac gacccgcact acacgctcct catcgaagaa ctgcaaatgg
    10441 tcgcacgctc cctgctcatc ttcggtctgc acgtccacgt cggcatcatt gaccgggagc
    10501 gacagattca cattatgaac gccgcgcgct atttcctgcc gcacgttctc gctctcacca
    10561 cttcgtcgcc cttctggctc ggcatcaaca ccggcctcaa atcctatcgc tccgaagtct
    10621 tcaagaaatt tccccgcacg gacatcccag accattacga ctcctacgca tcgttccagc
    10681 gatacatcaa gcttctgctc gacacgaact gcatcagcaa cccgcgcaag atttggtggg
    10741 acgtgcgccc gcatccggtt ttcgagacgc tggagtttcg cgtctgcgac atcccgacgc
    10801 gcgtggacga taccatcgcc atcgccgcgc tctttcaagc catcgtcgcc aagctcgaca
    10861 agttgatcga aaaaaatctc ggcttccgcc tctaccggcg cgcgttgatt caagagaaca
    10921 agtggcgcgc ggtgcgctgg ggcttggaag ggaagatgat cgacttcggc aagcagaagg
    10981 aggtgcccgt gcgcgatctg attcttgaac tgctagactt cgttgacgac gtgctcgacg
    11041 atctcggctc gcgcaaagag gtcgaacaca tccacacgat cctcgaacgc ggcacttccg
    11101 cagacgagca gttgcgtgtc ttcggcgaga cgaacgacct caaagccgtg gttgaccgcc
    11161 tgattacgcg gacgatggag aacgtcccaa ccattcagat ttaaacacca cgcgtttctt
    11221 cagcagcaaa ggtagagacg aaaaaagccc ccggtcacaa tggccggggg ctttttgaca
    11281 gctcgcgtgt tgaaaaattt atgccgcgtg attgagcgac tggcgcagga agttttcgaa
    11341 gatgatgcga ccgtcgcgtg attcgttcgg gtgctcccag cgcgtagcgc gcgtcttgtc
    11401 ccagtcctcg aaagattttt cgaggtggaa ctggacggtg tagatgagtt gcccgccggt
    11461 gcgcttgacc atcatctgat tgcggcagaa gtaggccgtg gcgagaagtt tgaaaccttc
    11521 cggaatacgg tcgagttgca gcccgtgatt ttgccagacg cgcaggatgc aggcttcgat
    11581 ggtgtaatcc tgccagatac ccgaatcgcg gtcgcccacg ccttcgaaga tcgggtcttc
    11641 ggagttggtg atgcggatga aacgatattg atactcgaac gggcggcctt cgcgtcgctc
    11701 gtgctgctcc atgttgtcga gcgttacgac gcgtgcgccg aagccgaggc cgacgagttg
    11761 atgcccgccg caaatgccga gcaccggaat cttcgtcgtg cgaatgaagc gaccgaaatt
    11821 ttcgacgatg ctcggatgat aataatcgaa gtcgcggagc gtgccgctca agacaatcgc
    11881 ctgcggctcg aactctgccg ccgcctctgc gatctccgag acgtgcgcga cgtgcgtttc
    11941 cggttcgcgc acgaggcgcg agatgttgtt gcggatattc ccgacggcca gacccgcgat
    12001 ctgccgctcg cgttggagat gcgacttggc gcggcgtccc cactcgccca ccgccatctc
    12061 cgacaagtcg cgctcatcgc gcagcaggtc gtctatgaca agcacgcgaa tcgcttcgat
    12121 gcgcttttca atcggctcga agcgacaaaa ggtgcgctgg ttgaacggga aaggaagtgg
    12181 ctcctcttga tccgggggcg tgattgtctc tttaacttct tgcatcatca aaaaataatt
    12241 taccagttcg ggattagatg ttcaaacgcg cggcggtcgt tgtttagtat tctggttgaa
    12301 ttgtggaatt tgtgtgccgg ctccggcctc ctcgatctcg cggcgcaaat ccgcgatgag
    12361 cgagttgcgc agacgcgtgc ggtaagcctt atttcgcgcg cccgccagat gctcgacgag
    12421 cagtagcatg cgttgccaac tcgattcgag ttcgtctgcg agatgagaga ttcgcgagat
    12481 gtcataaggg acggcgacgt tcgcgcaacc ggcgcgggca aatgtcgcca ggtaacgagc
    12541 cgttgccaga tcagggcagg acatgcggcg gcgaccgatt ttcaactcca cgccgagaag
    12601 cgtgtgctgc acgttcactt gttcgggctt agttgcgggc gtggcctgca aagcgtgtgc
    12661 gcgtgtgcgc aagggcagaa tccgctcgcg gtaaaagcgc ggcagccaga cctcgccgag
    12721 cgtctcttta atctgcgcgg ccaacgcttc gagcgagtcg ggcgcgcgcg atcttccggc
    12781 tctcttcgtt gcagatgaca taaaaaagtg acaagtgacg agtgacgaat gacaagttag
    12841 aaaagccgtg aatcgtgaat cgtaaatcgt gaataga
//
LOCUS       DASWNS010000157         6049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_188989, whole genome shotgun sequence.
ACCESSION   DASWNS010000157 DASWNS010000000
VERSION     DASWNS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188989"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..476
                     /locus_tag="VGB76_05370"
     CDS             162..476
                     /locus_tag="VGB76_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098366.1"
                     /translation="MWAYDFECDKTMAEIISALNESGPWQWVWRDKDAFGPYISSVPL
                     EGVSVRIYDLDGYYSNGPTYTADFKLGEGCKAEWATIDRVFRELLGNLPVRNLTIGEG
                     YD"
     gene            complement(684..1253)
                     /locus_tag="VGB76_05375"
     CDS             complement(684..1253)
                     /locus_tag="VGB76_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098367.1"
                     /translation="MKKLTCLTTASLLMAAWLSGCGDTERYRAEEQFGGIKKNLMASV
                     KELTSVPAKVELSDQPSIKGKVAVFMRKQLKSGPVNGGAYYMDIFYFRGLEEIYASKP
                     EEVGTIALVDCKMTDKGIYRTEDGREFPAEVEDCELTMIDRSKAAIVFKKMFEKTPDA
                     ERKSYTGNSVLHQSTNLEILPFLKDLPRT"
     gene            1773..2018
                     /locus_tag="VGB76_05380"
     CDS             1773..2018
                     /locus_tag="VGB76_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0098368.1"
                     /translation="MTHEYLAVMLGVRRAGVTEASVKMKEAELITYKRGDIQILDRAG
                     LEAESCKCYERMKAEYGRLFTNQPRPTDLMTPVTFAL"
     gene            complement(2002..2406)
                     /locus_tag="VGB76_05385"
     CDS             complement(2002..2406)
                     /locus_tag="VGB76_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098369.1"
                     /translation="MSTEQVTIFFIEPDDDTRRLLKRGLRASGYKVLVAEDLEDALEG
                     AEYGLARADLILLGPLGKSTEESLDIGRRIRGHAKHNGDTPLVVMAEKYGKDVEGTDV
                     NAGGNDWLFYLGEDAAQLRNMLARISPLKGRT"
     gene            complement(2403..2795)
                     /locus_tag="VGB76_05390"
     CDS             complement(2403..2795)
                     /locus_tag="VGB76_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098370.1"
                     /translation="MSTTEKRPTVMVVDDSEDIREVLRLQLTHLGYRVIEASNGREAV
                     EAVGEECPALILMDITMPVLDGLEATRMIRGMAEKCGVVIIAFTALHSSGNRERALAA
                     GCDGYEQKPLALGQLSNLLNRHLQGSIH"
     gene            3205..3918
                     /locus_tag="VGB76_05395"
     CDS             3205..3918
                     /locus_tag="VGB76_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0098371.1"
                     /translation="MHKTAKSSPPRNKILAALPDKEYQRLLPRLTPVSLSLGETLYEM
                     EGSIKHVYFPGSSVVSLVTHMDEGTSVEVGLIGGEGMVGLSVVMGDDVSQNHAIVQIA
                     DGAMRMTTAALRAELKHGGKLQSLLLRYSLTLLKQVSQTAACNRNHNLGERLARWLLL
                     CQDRVGGSEIRLTQEFLAQMLGTRRSRVSEAAIILQRAGLIRYSRGIITILDRGGLEE
                     FVCECYRAVKAEFDRLFSR"
     gene            complement(4393..5100)
                     /locus_tag="VGB76_05400"
     CDS             complement(4393..5100)
                     /locus_tag="VGB76_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0098372.1"
                     /translation="MSEPIRHPQQNHILASLPVEDYERLSPHLEAVELHHSQILYEAG
                     GKIDYVYFPSNSMVSLISQLSDGSSVEIGVTGYEGMVGIYAVLGVDRTPHEAMVQIPD
                     GAIRVKTSVVVDEFKRGGALHDLLLRYLQMLLLQTSQIAACNRLHTIGERLARWLLMS
                     HDRCVCDDLPFTHDFIAIMLGIRRAGVTEAAIILQTEEFIRYRRGHITILDRAGMEDY
                     ACECYRIVRDEFDRLVG"
     gene            5431..5814
                     /locus_tag="VGB76_05405"
     CDS             5431..5814
                     /locus_tag="VGB76_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098373.1"
                     /translation="MQNTKARILFADDHDDIRFMVTTLLGAYDYEVVTADCMVSALEL
                     AHSAAFDVYLLDNQLPDGTGSELCQRLREFDSDTPIVFFSGDAYESDRQRAFNSGAQE
                     YVEKPDLSALKTAIARVLRTPQLVH"
BASE COUNT         1330 a         1669 c         1731 g         1319 t
ORIGIN      
        1 gagggtggcg gagcgtcgtc tctatcaggc gacggcgatg ccgaacaacg gcatgcacac
       61 ggccgcgaat agcgcggctc tcatccacaa ggcttgtccg tatcggcgtt gaatgcgcgg
      121 cgggtgatgc cgagcgttag gccgccgggg taagagagtc tatgtgggct tatgatttcg
      181 aatgtgataa gacgatggcg gagatcatct ccgccctcaa cgagtcaggg ccgtggcagt
      241 gggtctggag ggataaggac gccttcgggc cgtatatttc ttccgtaccg ctcgaaggcg
      301 tgagcgtccg gatatacgac ctcgacggtt actacagcaa cgggccgaca tataccgctg
      361 acttcaagct gggcgagggg tgtaaggccg agtgggcgac gattgatcgc gtctttagag
      421 agctgctcgg caatctgccg gtgcgcaacc tgacgatagg tgaaggctac gattagcccg
      481 gtgacctaac gacggcatgc tcccgacggc gaacagcgtt gccttcgtgc gccaacaatc
      541 tgccagataa ccctcattaa caggccatcc taaaccgctc gtatgttgcc ctccgcgcgt
      601 caccgatagg gtcaaagctt tggatatggg actccacttt cggcccgtct tggctaacac
      661 ggcgcgcacg agtttttgat tggctaggtt ctgggtaggt ctttcagaaa aggaagtatc
      721 tcgaggttgg ttgactggtg caggacggaa ttgccggtgt aggatttacg ttccgcgtcg
      781 ggggtcttct caaacatctt cttgaagacg atcgccgcct tcgagcggtc gatcatcgtc
      841 aactcgcagt cttcgacctc ggccgggaac tccctcccgt cttcagtcct gtagatgcct
      901 ttatcggtca tcttacagtc gactagggca atcgtcccga cttcttcggg ctttgaggcg
      961 tagatttctt ctagtccccg gaaataaaaa atgtccatgt aatacgcgcc gccgttgacg
     1021 ggtccgcttt ttaactgctt cctcatgaag acggcgacct ttcctttgat ggagggttgg
     1081 tcgctaagtt ccaccttcgc cggtacgctg gtcaactctt taacagaagc catcaggttc
     1141 tttttgattc ccccgaattg ctcctccgcg cgataccttt ctgtgtcccc gcaacctgag
     1201 agccaagccg ccatgagcag cgaagcagta gtgagacatg tcaacttttt catcgcgctt
     1261 atacctttcc gtagaggcgg ctctgtctga gcgggagtga ggtctaattt aatagcgcgc
     1321 tacttatatc cctggctagc cgtcccgtca attcttgggc cgacgtatga gcgcaagtaa
     1381 tagagatgat tgagcggaat ttttgatggg ctgaccgttt gggcttcaat gtcgaaagga
     1441 gatgtgtcat ggcatttcaa tcagaacatt gtattgagtg cgacgaaccg ggtaatcgcc
     1501 tgaccaaagg caatggtaaa tgtaatctgt gtcgaggatc gggggttgat gtgggcgtgg
     1561 cggtgctagc cgggtatgag ggcgaatgcg aaagatgcaa cggaacgggg gtatgtcccg
     1621 cttgtaatgg aacgggtgaa gttagttgac atattaatag aggccgtagt gggagggacg
     1681 tgcagagtag aaacaataga aatgaatcgc gaagaattac tctccctcgt ccccgcgact
     1741 aacaacgcat tcaagccgat gcctcgatag cgctgactca cgagtacctc gccgtgatgc
     1801 tcggcgtgag gcgcgcgggc gtgaccgaag cgtctgtaaa gatgaaggag gcggaattga
     1861 ttacctacaa gcgcggcgac attcagatac tcgaccgcgc ggggcttgag gcagagtcgt
     1921 gcaagtgcta cgagaggatg aaggccgagt acggacgcct gttcacaaat cagccgcgtc
     1981 ccaccgacct catgactcct gtcacgttcg ccctttaagg ggcgagatgc gcgctaacat
     2041 gttacgcagt tgggcggcat cttccccgag gtagaagagc cagtcattcc cgccggcatt
     2101 tacgtccgtg ccttccacgt ccttgccgta cttctcggcc atcacgacca gcggcgtgtc
     2161 gccgttatgc ttcgcgtgcc cgcgtatccg ccgtccgatg tcgagcgact cttcggtgct
     2221 tttgcctagc ggcccgagca ggattaaatc cgctctcgcg aggccgtatt cggcgccctc
     2281 aagcgcatcc tcaaggtctt cggccaccaa caccttgtag ccgctcgcgc gcaggccccg
     2341 ctttaaaagc cggcgagtgt cgtcgtccgg ctcgatgaaa aatatagtta cctgttccgt
     2401 gctcaatgaa tactcccttg taggtgccgg ttaagaaggt tagacagttg ccccagggcg
     2461 agcggcttct gctcgtagcc gtcgcagccg gccgcgagcg ccctctcccg gttcccggag
     2521 ctgtggagcg cggtgaaggc gatgatgacc acgccgcatt tctccgccat tccgcggatc
     2581 atgcgggtcg cttccaagcc gtcgaggacg ggcatcgtga tgtccatcag gatcagcgcg
     2641 ggacactcct cgcccaccgc ctcgacggcc tcccgcccgt tcgatgcttc gatgacccgg
     2701 tagccgaggt gcgtcagttg caaccgcagt acctcgcgga tgtcttccga gtcgtccacc
     2761 accatcacgg tcgggcgctt ctctgtcgtg ctcatgcgtt ccccttgtgc ggactgcggg
     2821 acggctcaga cgccgaggcc cgcgaacgtt cacgccatta agggtgctcg ctggggaaag
     2881 attaaggctg gagcgggagg gttccgggtt agtggtcgga gcctagggct gaaggattaa
     2941 tgtttcggca gaaaacaact taatgagaac actgtacctt actcggtggc ggcgagtaaa
     3001 ggtatatgcc cgaattcggt catatagcca tatgcccgaa aaagcgacat acggccgatg
     3061 acgtgataag atggaatcgt atttaagccg taccgttctt ttagaatcca actttcgaca
     3121 tcaggacttg cccgaacacg ggcatcttct cttgccgtcg gctgtgttca cacacgctac
     3181 ccggtcaaga aggagatgcg ccccgtgcac aaaaccgcca aatcgtcacc gccccggaat
     3241 aagattctcg ccgccctgcc ggataaagag taccaacgcc tgctgccccg cctgacgccc
     3301 gtctccctga gcctgggtga aaccctctac gagatggagg gcagcatcaa gcacgtctat
     3361 ttccccggca gctccgtcgt ctcgctcgtc acccacatgg atgaggggac gagcgtggag
     3421 gtgggcctga tagggggcga ggggatggtc ggcctctccg tcgtaatggg ggacgacgtc
     3481 tcgcagaacc acgccatcgt gcagatcgcg gacggggcga tgaggatgac gacggccgcg
     3541 ctcagggcgg agctgaaaca cgggggcaaa ctgcaaagcc tgttgctgcg ctactcgctc
     3601 accctgctca aacaggtctc gcagacggcg gcctgcaacc gcaaccacaa cttgggagag
     3661 cggctcgccc gctggctgct gctgtgccag gacagggtcg gcggcagcga gataaggctg
     3721 acgcaggagt tcctggcgca gatgctcggc acgcgacgct cgcgcgtgag cgaggcagcg
     3781 atcatcctgc agcgcgccgg gctgatccgc tactcgcgcg gcataatcac catcctcgat
     3841 aggggaggct tggaggagtt cgtgtgcgag tgttaccggg cggtcaaggc cgagttcgac
     3901 cgcctattca gtcgttaaaa gaggcaagac aactcggtgc ggagtaaaga aagatgaatt
     3961 ccacagaaga accttttgtg gaattaaatt ctgaaagcta cgaaatctat ttttagcctt
     4021 agcgttgctc ctttccctca gtcgcagtca attaagacct gtcctatgtg tattagcaca
     4081 ccgactgcca aagacaggcg agctgttata aataatccct ctctttaaac agtaataatt
     4141 aagagtggtc tgagtggcgg ccgagccagg tgttccctgt cgccgactct aaaggtctgt
     4201 tatgacgatg ctccaacccg aacagcaaac catctcgcct acgttgaagg agctgctcga
     4261 gcgaaccacc gatgagatcg cccgacgctg gttgttggcg gtgcgtgagg atcgcttccc
     4321 cctcgttgaa tagaaacacg acgtggcaaa agattcgtgt ctgcacacag tctgctctat
     4381 tcggcaggta gctcaaccga caagtcggtc aaactcgtcc ctcacaatcc ggtagcactc
     4441 gcaggcataa tcttccatcc ccgccctatc cagtatcgtg atgtggccgc gccggtatct
     4501 gatgaattcc tcagtttgca ggatgatggc ggcctcggtg acacccgcgc ggcggatgcc
     4561 gagcatgatg gctataaagt cgtgcgtgaa tggtaagtcg tcacacacgc acctgtcatg
     4621 gctcatcaac agccagcggg cgaggcgttc tccgatagta tgaagacgat tgcaggccgc
     4681 gatctgactc gtttgcaaca gcagcatttg tagatagcgc agcagcaaat catgtaacgc
     4741 accgccccgc ttgaattcgt ccacaacaac gcttgtcttt acccgtatcg cgccgtcggg
     4801 tatttgcacc atagcctcgt gcggcgtcct gtcaactccc aacaccgcgt agatgccgac
     4861 catcccctcg tatccggtga cgccgatttc gacgctcgac ccgtcggaca gttgcgagat
     4921 aagggaaacc atcgagttcg aagggaaata gacgtaatca atcttgccgc cagcttcgta
     4981 gagaatctgg ctgtggtgga gttcgacggc ttccagatgt gggctgaggc gttcgtaatc
     5041 ttcgacgggc aaagatgcga gtatgtggtt ttgctgtgga tggcggatag gttctgacat
     5101 taggatttcc cttcttgcga ctgcacgtgt gaaaacacag cagacggcac aaggagaagg
     5161 acgtaaaact cttcccaatg tcgaatggtg ggttggtaaa taactgtcgg acaactgacg
     5221 tgagcattat agcaccgaaa ggcgatttcc cgtactaagt cgtacagact cctagcccca
     5281 atcggcgtta taagtgcgtc cgtaccataa gctaagagga aggcagtaga tactactcag
     5341 cacgcctgga cgttggagta gcacgactgc ggtgctgcat cactctccct tccccgacac
     5401 aatttttact accgtccaaa actctccgtc atgcagaaca caaaggctcg catcctcttt
     5461 gccgatgacc atgatgatat tcggttcatg gtaactaccc tgctcggcgc gtacgactat
     5521 gaagtcgtca ctgccgattg catggttagt gctttggaac tggcgcatag cgcggccttc
     5581 gacgtatatc tcctcgataa tcagttaccg gatgggacgg gcagtgagtt gtgccagcgg
     5641 ttaagagagt ttgacagtga cacgccgata gtcttcttct ccggagacgc ctacgaatcc
     5701 gacaggcaaa gggcgttcaa tagcggggca caggagtatg tggagaagcc cgacctttct
     5761 gccttgaaga cggcaatcgc acgcgtgctc cgaaccccgc aactggtaca ctgaccacaa
     5821 agcatagttg tcgctttcgc ctaacaatcc attgaccgca aggggctgga aagaacgtcg
     5881 tgcgaatgtt acccgatgat gaagaaagag ttcacacgcc tactcgacag caacgggcac
     5941 gcactgtgaa aggaagcccg cgcgcatcat caatcttgca gcaagcggag cctccacagg
     6001 ctatgaatcc tacctgcatc gaaatgtaac tggaagtaca taatggatg
//
LOCUS       DASWNS010000158        12699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_240243, whole genome shotgun sequence.
ACCESSION   DASWNS010000158 DASWNS010000000
VERSION     DASWNS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12699
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240243"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VGB76_05410"
     CDS             complement(<1..139)
                     /locus_tag="VGB76_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEY0098374.1"
                     /translation="MLLYVRLYCRYQLSYRAVEETMRERGLDVNHSTAFRWVQRYAPE
                     IN"
     gene            369..1790
                     /locus_tag="VGB76_05415"
     CDS             369..1790
                     /locus_tag="VGB76_05415"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0098375.1"
                     /translation="MKSKDRILVVDDESSITGALEMILGEQGYNLFTAASGLEAAELL
                     ARHSFNLVFVDLRLTDITGLELLTRIKHDSPDTEVILMTGYGSVDVTIEAIKKGAYYY
                     LEKPFTPDQVVMLTARALQYAAVQRENQTLRRTLANASKTFGIIGCSPQMRHIFETIR
                     ATANSDASVLIEGESGTGKELIATAFHLQSDRSKQPCTRINCAAIPPELIESELFGYK
                     KGAFTGADRDKRGLIEASEGGTLLLDEIAEMPVHLQTKLLRVLQERKVRRLGDEREIE
                     VNFRLICATNRSTSQSVEEGLLRKDLYFRISTIKIEVPPLKERPDDLALLVEHFLQRY
                     NKKYYASEHDISPSAHALLMHYDWPGNVRELESVIERAVLFCREGQLLPEHLPKHLQE
                     AEASPFKCVIPSYMTLAEIEHEVIYQTLKRTGGNMKKTAQILNLHRPTLYRMLKRMGG
                     NKEPKSVGAPHSLPVGAISSSDS"
     gene            2405..3700
                     /locus_tag="VGB76_05420"
     CDS             2405..3700
                     /locus_tag="VGB76_05420"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY0098376.1"
                     /translation="MSKLDSTLMAVLSQHGFTGSIESTLEDRLGRRIDAKRADLGRQL
                     WFDTITALNKDNSCSGCHAPTAGFGDTQSIAIGIESNGVVGRDRRGPRNQRRTPMAIN
                     TAFFPTLMWNSRFFSLSGDPFDNSAGFQFPLPEGLSLSNLPHLLVAQAHIPPVERNEA
                     AGFDFPGDNFVLRTEVMRRLNENDEYRKLFGQVFGEVRRGAPIDFEMFGKAIAEFEFT
                     LTFADAPIDKFARGDRDEMTDEQKKGALLFFGKANCAQCHTVAGNSNEMFSDFKQHVV
                     GVPQIFPRETNSVWDGEGGNEDFGLEQVTQNPADRYLFRTSPLRNVALQPTFFHNGSF
                     NRLEDAVRHHLNVFQSARGYTPARLDRDLTGPLAPIEPVLARLDSLLVSPVHLTDDEF
                     RQLVEFVRGGLLDPRAKPENLRKLIPKRVPSGRPVHVFQ"
     gene            4551..9737
                     /locus_tag="VGB76_05425"
     CDS             4551..9737
                     /locus_tag="VGB76_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY0098377.1"
                     /translation="MIPACGKTVTARVVAFEQIHTYNRFGAFNPHGLMYALVRDVVGS
                     TPGGVSLRATKRPRPIVLRVNEGDCLQITFTNYLSPTQPAASPEPGEIDGGSAPRTRH
                     ASIHVNGLDYLNIASDGANVGRNASSLAAPGETKIYSLYAKKQGQYLMYSMAAPAGGE
                     GDNGQPGLGLFGSVNVEPKNSKWYRSQVTAAQLQAVTTGQTAKGTPIIDYEKVDASNT
                     PILNMLNGSNEIVHSDINAIITGANGALLEEDCTKAPPTGTCGEPFREFTAIFHDEIK
                     AVQAFAELNEEMFHGVRDGFAINYGASGLGSALIANRKKVGPAKNCDECKFEEFFLES
                     SVNGDPAMLVKKNLNGVATQALYPDDPSNVHHSYLGDPVRFRNLHTGAETHVFHLHAH
                     QWLQSPRDENSTYLDSQTIGPGGAFTYEINYGGSGNRNLNPGDSIFHCHLYPHFAQGM
                     WELWRNHDAFEAGTPDRNLPDAEIAGGTPNPAVVPIPGRALAPMPAANFKGFPFYIMG
                     QAAIAGHRPPQPPLDMEWDGGLPRHISVHSPVVLNGEPAIEAGLLNDPVAANVLSQIT
                     NDDEVLQFARRELQSKLVLIDPLGTPDEKNAMKFHEGTLTFPNGTVGFPDTRTFPAPV
                     EAWQGAAYWSFTASGAPAKFFVNGHTRQPGAPFADPCRKNPTFVRQYRAAYIQFDMTV
                     NRAKWHDRQARITVLEQDVNSTLNGTRSPEPFFFRANSGDCIQFAATNLMPEVLNLDD
                     FQIFTPTDTVGQHIHLVKFDVTASDGAGNGWNYEDGTFAPGEVRERIEANNAYQTATG
                     GSQFLHPKTHPRLGAGPNGEWIGAQTTIQRWWADPLVNTLGQDRTIRTVFTHDHFGPS
                     SHQHHGLYGALVVEPAGSTWKALNNTTTLGGPNTQVRPDGGPTSFAANIITNPSSKSF
                     REFNLAFADFAIVYTADLKPVNPPGRKEAALPIAIEPTIPMPEAISAADPGTQLVNYR
                     NEPIPLRIGRYSNGNFVQKTGQAGDLAYVFSSFTHNDPFTPVLTAYEGDRTMIRLIQG
                     AQEEQHVFTAHGIKWLFEPSSPNSGYRNAQQIGISEHFEMELSPLPVLNHNPAWYNSA
                     YYDKGKNFADHLYASSGTDNLWDGQWGILRIYQGNQPNPIRLAVPTNQGVEYTSPRLM
                     TTNTMESSTMVDGTTMTPEATTDSQISSGAISSEAVTVNTSNPDGFDLLAPLPNNLDG
                     RSGSVATTTTTTDPVYSPAEPDSTGTGTVTDQKTAVYTDGTSMTQPSTMQSTDGTAMI
                     ITESPSLVIDPMFSTSTLRKPPSTSLASVCPVGAPPRTYNVSAYLASSIVPGGALVYN
                     QRFGIKDSKAIIFVENSDVASIQNGTRRPEPLILRAAAGECISVTLTNKLPSVVPEIN
                     SWNMMPMIVNRFNFNQVKTSNRVSLHPQLVSANTYKDDASAVGFNQDSTVGPGQTRTY
                     TWYAGNRVVNSLGNYVQAPVEFGATNLRDMGDVIKHSSHGAIGSLIIEPVGARWSYPV
                     ANSKATADVASPTGTFREFVVIYQDDLTVQKNGVPLPNIGETDDSEDSGMKAFNYRTE
                     PLWGRLGFSIKEGDTNNFDYKDALSSTASLPGCGGVCGDPETPVFTATAGVPVRFRVL
                     NAAGHPRQHAFALFGHHWNFSPFTQLSTVQGFNPFTFEVGAYDGIGPTRHLNILTAAG
                     GLRRVAGDYLYRTMDSPNFAGGGLWGIFRVVKAVRILTPIESDPVLYTEPQPAPAVIE
                     NQ"
     gene            9959..11944
                     /locus_tag="VGB76_05430"
     CDS             9959..11944
                     /locus_tag="VGB76_05430"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY0098378.1"
                     /translation="MRVAKLAASVLLLAASTAHAQIGGPHDAARAAAKKAPVKAPASV
                     SQRFEREGVAVDFSLTASPDAQGQNPGLVAGSDATVTFRLSDARTGAPVTGLHPTGWI
                     SSSTSPHAPNEAQCKDRIKTLAGGLLSTRAEIDLNSYVVLTINHDNTISFINPLVSFS
                     KTKLENLISLPGAGADWALTRQKDLLYVTLPEQSAVAVIDTATRKLKGQVATGEKTRP
                     VRVQLQPDGRYLWVGLDGSSKIAVIDAQAARLVTTLTVGEGLHNITFTADSSFAYVTN
                     SASDTVSVIETQRFVKVADIKVAKTPVAVAYSSASKLVYVASVNGETISVIDPAKQQA
                     VANVQVGRGVVALRFEPKGRYGFAVNQVGSTVSVIDAATNSLVGTTAVVKEPDQVVFS
                     DEYAYVRGLGSEKFSLIELREVPSGKISPIYIQAGQRPATDVPKEIGVADMIAPVPGG
                     DGAMIANTADAMFYYYTEGMMAPMGSFTNYKRRPRALLILDRSLSEVTPGVYSTPIKL
                     TSAGRFDVPFILDQPRIINCFQLSVAESNDKAVKQQVAGSLLIEPLFKGAQFNMGERV
                     KLRFKITDTDTRQPLAKLKDVRVLVFEPPGVWQQRQWAKEVGNGVYEVEQEFPNDGVF
                     NVMVSIFSRGIEFSDLPFTVVAVEEKKASGQAKVNAN"
     gene            11962..>12699
                     /locus_tag="VGB76_05435"
     CDS             11962..>12699
                     /locus_tag="VGB76_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0098379.1"
                     /translation="MRRNMKSTRALVALFFVAFICAAGTESVSAHASQKGRARATRTG
                     VEYTCPMHPEVKAKRPGTCPKCRMDLRRVSVAPQAAVPADNRNSATPERAGSSSNSSA
                     TPEAGLRIPDTNVLDQDGRRLNFYSDLVKGKTVAIDFIFTTCNGVCPTMTAKFRQLQQ
                     ALGDRVGRDIQLISISVDPTTDVPARLHAYAAKFHAGPGWTFVTGDKPEIDALLRALG
                     AFAPDKTKHPQTILIGNEAANYWTRTLG"
BASE COUNT         2816 a         3895 c         3489 g         2499 t
ORIGIN      
        1 tgttgatttc gggagcatat ctctgcaccc acctgaacgc ggtcgagtga tttacgtcga
       61 gtccacgttc acgcatcgtc tcctcaacag cgcgataaga aagttgatac cggcagtaca
      121 atcgaacgta tagaaggata aattcgggcg ggtaaggccg ccgcttaaaa ggcgaggagg
      181 tatgtttcag gaacaatcct gatatgacga gtggagagaa cttgagagag ggataatacc
      241 tgaatgacta ttcttgcaac actgccgtaa ggagtctctc gccgcttatc tttcctgcgc
      301 ccggtctaaa gttgcacttc aaagttggat ttacgatcaa gctggcggcg gcaataagat
      361 taagagagat gaaatcaaaa gatagaattt tagtggttga cgacgagagc agcatcaccg
      421 gcgcgctgga gatgattctc ggagaacagg gctacaatct gttcacggcg gcgagcgggc
      481 tagaggcggc ggaactgctc gcccggcact ctttcaattt ggtctttgtt gatttgcgtc
      541 tcaccgacat cacaggactc gaactgctca cgcgcatcaa gcacgacagc cccgacacgg
      601 aagtcatcct gatgaccgga tatggttctg tggatgtgac tatcgaagcc atcaagaagg
      661 gcgcgtatta ttatctggaa aaacctttca cgcctgacca ggttgtgatg ctcacggcgc
      721 gcgcgctcca atatgccgca gtgcaacgtg aaaaccagac gctcaggcgg acgcttgcca
      781 atgccagcaa aaccttcggc atcatcgggt gcagcccgca gatgcgccat atctttgaaa
      841 ccatccgcgc caccgctaat tccgacgcgt ctgttttgat cgagggtgaa agcggcacgg
      901 gcaaggagtt gatcgccacc gcatttcacc tgcaaagcga tcgttccaag caaccctgca
      961 cgcgtatcaa ctgcgccgct attccgccgg aactgatcga gtcggagttg ttcgggtaca
     1021 aaaaaggtgc ctttacaggc gcagaccggg ataagcgagg actgatcgaa gcttcggagg
     1081 gcggcacgct gcttctggat gagatcgcgg agatgcccgt gcatctccaa accaaattgc
     1141 tgcgcgtgtt gcaggagcga aaagtgcgcc gcctcggcga cgagcgggaa attgaggtca
     1201 attttcgttt gatctgcgcc accaatcgca gtacgtcaca atcagtagag gaggggttac
     1261 taaggaaaga cctctacttt cgcatcagca ccattaagat tgaagtgccg ccgctcaagg
     1321 aacgtcccga tgatctggcg ttgctggtag agcacttcct ccaacgctac aacaagaaat
     1381 attatgcaag cgagcacgac atctctccgt ccgctcatgc tctgttgatg cattacgatt
     1441 ggccgggcaa cgtgcgcgaa cttgaaagcg tgatcgagcg cgcggtgctg ttttgccggg
     1501 aagggcaact gttgcccgaa catctcccga aacatttaca agaagccgaa gcgagtccct
     1561 tcaaatgtgt cattccatcc tacatgaccc tcgcggagat cgagcacgaa gtgatctacc
     1621 agacgcttaa acgcaccggc ggtaacatga aaaagaccgc gcaaatactc aacctgcacc
     1681 gcccgacgct ctatcgcatg ttgaagcgca tgggcggcaa caaagaaccg aagagcgtgg
     1741 gcgcacctca ctcgctgcct gtaggcgcga tttcgtcaag cgactcataa gtcagcgctg
     1801 tggcgcagta atgtgcgctt gtttcataat gaaacataga aagacccgcc attggcgcgg
     1861 ctaattgctg aaagcagtct catcgctata aacggctgta aaagtagcat ccggataagt
     1921 gggcacaacg tttgcttagc agagttcgtt cttaccgacg gagaagttct gttgcccggc
     1981 gatgtggaca tgaatttgcg gttaacgaag tcctgttaaa tttaacccgg tctttaaccg
     2041 cgaagtaacc tgctgatccg ctccacgtct cctctaaaga ccttgcatct ttacttaaga
     2101 gcgcgcattc gaggatcgcg cacgcgtctt cgtcctgagg ttcagttccc ttgcgagccg
     2161 ccggctcttc ttctgtcaca acacatttca gggagacctt ccgttgaatt tgaaattcta
     2221 taagctattg agtcttgcgc tgctcttcgc attcatcggc gtgattaagt ttgcgcgtcc
     2281 ggtagcggga agcaacgatc tccgcgcgtc cgtttcagct cagagagcgg gcgagcttcc
     2341 tccagtccaa cagagcaaag acgagaataa gtcgaaaccg aacaagggat cgaagggggg
     2401 taaattgtcg aaactcgact caaccctcat ggctgtgctc agccaacatg gtttcaccgg
     2461 cagcatcgag agcacgctcg aagaccggct cgggcgtcgc attgacgcca aacgcgcaga
     2521 cctcggacgc caactctggt tcgataccat cacggccttg aacaaagaca atagctgttc
     2581 cggctgccat gcgccgaccg ccggttttgg cgatacgcaa tcaatcgcca tcggtatcga
     2641 aagtaacggc gtcgtaggcc gcgaccgtcg cggcccgcgc aaccagagac gcacgccgat
     2701 ggcgatcaac accgcgtttt tccccaccct catgtggaac tcacgcttct tctcgctctc
     2761 cggcgacccc tttgacaaca gcgcgggctt ccagtttccc ttgcccgaag gactctcgct
     2821 ctccaatctc ccgcacctcc tcgtggcgca ggcgcacatt ccgcccgtcg agcgcaatga
     2881 agccgccggt ttcgattttc ccggcgacaa ctttgtgctt cgcacagagg tcatgcgccg
     2941 cttgaacgaa aatgatgagt atcgaaaact gttcgggcaa gtctttggcg aagtgcgtcg
     3001 gggcgcgccg attgatttcg agatgttcgg taaagctatc gccgagtttg aatttacttt
     3061 gacgtttgcc gatgcgccca tcgacaagtt cgcgcgcggc gaccgcgacg agatgacgga
     3121 tgaacagaaa aagggcgcgc ttcttttctt cgggaaagct aactgcgcgc aatgccacac
     3181 cgtcgcggga aactccaacg agatgttcag cgacttcaaa caacacgtcg tcggcgtgcc
     3241 gcaaatcttc ccgcgagaaa ccaattcagt gtgggacggt gagggcggca atgaggattt
     3301 cgggttggag caggtcacgc agaatccggc ggatcgctat ctcttccgca cgtcgccgct
     3361 gcgcaacgtc gccctgcaac cgaccttttt ccacaacggc tcattcaaca gactggaaga
     3421 cgcggtgcgc catcatctca atgtttttca atcggcgcgc ggctacactc cggcgcggct
     3481 ggaccgcgat ttaaccgggc cgctcgctcc catcgagccg gtgctggcac gtctcgattc
     3541 acttttagtg tcgcccgtcc atcttacgga tgatgaattt cgccagcttg tggaattcgt
     3601 ccgtggcggg ctgctggacc cgcgcgccaa accggagaat ttgcgtaagc tgattcccaa
     3661 gcgcgtgccg agcggtcgtc ccgtgcatgt ctttcagtaa aaattagggc gttcaaataa
     3721 agcagcgaga acctgaccga cgttacatca aggtcaggtt ctcgctgttt gtgcgttcat
     3781 caaagcatct cgcccgctgc tttaaccaaa ccacgcccgg tccaattcgt atcacggaaa
     3841 ctctcagagt gtttcagcct gaaacacaaa ttcgattgcc tcaacccgac cttaatattc
     3901 tgttttcgtt acaaagtgcg tgtgctttgc tgatttattg gcaggctatg tgaaggggca
     3961 cggctattgc tctaaggggt aacgtgcgta gaaagttagt ctaaacactc atcaatccgg
     4021 gcttacacaa gcacggctcc tcaagcctta tcgaagggga gcgttgagtc tgtaccctac
     4081 cttttccaaa ccgtttgagt gtaatcctgc cacccctgtt tgcttaaaac agcacacagc
     4141 agaagcagcg gtgttgtcag aaccgcttgc gcatcgaatg tggctcctga ttcgttcagg
     4201 caggattagc tcgtagtcgc ttctgatccg ttcttcacta accactttaa ggagacagca
     4261 tgaaggaaag tcccccaacc tactctcgcc catggcgacc gatccgtgga agtttcttcc
     4321 tgatcagtct gctgatgctc tgggcgggaa cggcgtttgc gcaaacgcct gtctcaacag
     4381 tagacacaat tcctgaagat ggtagcaccg aggttataac aaaacccgtc atctacccga
     4441 caccattccc aactccaact ccattcgcga cccccacccc gacgccaatc gcgactccga
     4501 cgccgagcgc gactccgaca cccgtacctg caccgacgcc aatagcaggc gtgatacctg
     4561 cgtgcggcaa gacagtcacg gcgcgcgtcg tcgcgtttga gcagatacat acctacaacc
     4621 gtttcggggc gttcaacccg cacggcttga tgtacgcgct cgtgcgcgat gtagtgggca
     4681 gcacgcccgg aggcgtgagc ctgcgcgcga ccaagcgccc gcgtcccatc gtcctgcgcg
     4741 tcaacgaagg cgattgtctg caaataacct ttaccaacta tctcagtccg acgcagccgg
     4801 cggccagtcc cgagccgggc gaaattgatg ggggcagtgc gcccaggacg cgccacgctt
     4861 caatccacgt caacggtctg gactatctca acattgcttc cgatggggcg aacgtcggta
     4921 ggaacgcctc gtcgctcgcc gcgcccggcg aaaccaaaat ctacagcctc tacgcgaaaa
     4981 aacagggaca gtacctcatg tactcgatgg ccgcaccggc aggaggtgaa ggcgataacg
     5041 gccagcccgg cctcggcctg ttcgggtcgg tcaacgtcga gccgaagaat tccaagtggt
     5101 atcgctcaca ggtgacagcc gcacagttgc aagccgtcac cacggggcag accgcgaagg
     5161 gcacgcccat catcgactac gaaaaggtcg atgcgagcaa cacgccgatt ctcaacatgc
     5221 tcaatggcag caatgagatc gtgcacagtg atattaacgc catcatcacc ggcgcgaacg
     5281 gcgcgctgct ggaagaagac tgcaccaaag cgccgccgac cggcacttgc ggcgaaccct
     5341 tccgcgagtt cacagcgatc ttccacgacg agatcaaagc cgtccaggca ttcgctgaac
     5401 tcaacgagga gatgttccac ggcgtgcgcg atggcttcgc catcaactac ggagcctccg
     5461 gcctcggctc ggcgctcatc gccaaccgca aaaaagtagg ccccgcaaag aactgcgatg
     5521 agtgtaagtt cgaggagttc ttccttgagt cgtcggttaa cggtgatccg gcgatgctcg
     5581 tgaagaagaa tctcaacggc gtcgccacac aggcgctcta cccggacgat ccttccaacg
     5641 ttcaccactc ttatctcggc gaccctgtgc gcttccgcaa cctgcacacc ggagccgaaa
     5701 cgcacgtctt ccacttgcac gcgcaccaat ggctgcaaag cccgcgcgat gagaattcca
     5761 cttatctcga ctcgcagacc atcggacccg gtggcgcgtt tacctacgag atcaactacg
     5821 gcggctccgg caaccgtaat ctgaatccgg gcgattcgat cttccactgc catctctacc
     5881 cgcacttcgc gcaaggcatg tgggagttgt ggcgcaacca cgatgccttc gaggccggta
     5941 cgcctgaccg caacctgccc gacgcggaga tcgcgggcgg gacgcccaat ccggcagtcg
     6001 tgccgattcc cggtcgcgcg ctcgcgccca tgccggctgc aaacttcaaa gggtttccgt
     6061 tctacatcat gggtcaggcc gccattgccg gccaccgtcc gccgcaacca ccgctcgaca
     6121 tggagtggga tggcggtttg ccgcgtcaca tcagcgtgca ctcgcccgtg gtcttgaacg
     6181 gtgagcctgc gattgaagcg ggactgctga acgatccggt cgcggcgaac gtcctgtcgc
     6241 aaatcaccaa cgacgatgaa gtgctacagt ttgcgcgtcg cgaactccag tcgaaactcg
     6301 tgctgatcga tccgctcggc acgccggacg agaagaacgc gatgaagttc cacgaaggga
     6361 cactcacttt cccgaatggg acggtcggtt tccctgatac gcgtactttc cccgctcctg
     6421 tcgaagcctg gcagggcgcg gcttactggt ctttcaccgc ttccggtgct ccggccaagt
     6481 ttttcgtcaa cgggcacacg cggcaacccg gcgcaccttt cgccgacccg tgccggaaga
     6541 atcccacttt cgtgcgccaa taccgcgccg cttatattca attcgatatg acggtcaatc
     6601 gtgcgaagtg gcatgatcgg caagcgcgca tcacggtgct ggagcaggat gttaattcga
     6661 cgctcaacgg cacacgttca cccgaaccct tcttcttccg cgccaactcc ggcgactgta
     6721 tccaattcgc agctaccaac ctgatgcccg aggtgctcaa tctggacgac ttccagattt
     6781 tcacgcccac ggatacggtc ggccagcaca tccacctcgt caagttcgac gttactgcgt
     6841 ctgacggagc gggcaacggc tggaactacg aagacggaac gttcgcaccg ggcgaggtgc
     6901 gcgaacgcat cgaagccaac aacgcttacc agacggccac cggcggctcg cagtttctgc
     6961 acccgaagac gcacccgcgc ctcggcgcag gtcccaacgg cgagtggatc ggcgcgcaga
     7021 ccacgattca acgctggtgg gctgacccgc tcgtcaacac gctcggtcag gatcgcacga
     7081 tccgcaccgt cttcacgcac gatcacttcg gccccagttc acaccagcat cacggtcttt
     7141 acggcgcgct cgtcgtcgaa ccagccggct caacctggaa agccttaaac aacacgacga
     7201 cgctcggtgg accgaacact caagttcgtc ctgatggtgg tccgacttca ttcgcggcca
     7261 acatcatcac gaaccccagc tcgaagagtt tccgcgagtt caatctggcg ttcgccgatt
     7321 tcgccatcgt ctacacggcg gatttgaaac cggttaaccc tccgggacgg aaagaggcgg
     7381 ctctgccgat agcgattgag ccgaccatcc cgatgcccga ggcgatctca gccgccgatc
     7441 ccggcacgca actcgtcaac tatcgcaacg agccgatccc tttgcgcatc ggtcgctata
     7501 gtaacggcaa cttcgtgcag aagaccgggc aggcgggcga tctggcctac gtcttcagtt
     7561 ccttcaccca taacgatccc ttcacgccgg tcttgacggc ttacgagggc gaccgcacga
     7621 tgatccgcct gattcagggc gcgcaggaag aacaacacgt cttcacggcg cacggaatca
     7681 agtggctgtt tgagccttcg tcgccgaact ccggctatcg caatgctcag cagatcggca
     7741 tctccgagca cttcgagatg gagcttagtc cgctgccggt gctcaatcac aatccggcgt
     7801 ggtacaacag cgcctactac gacaagggca aaaacttcgc cgatcacctc tacgcctctt
     7861 cggggacgga caacctgtgg gatggtcagt ggggcatcct gcgcatctat caaggcaacc
     7921 agccgaaccc gattcgtctt gccgtgccga ccaatcaggg agtcgagtac acctcgcctc
     7981 gcttgatgac caccaacacg atggaaagtt cgacgatggt tgacggaacg accatgacgc
     8041 ccgaggcgac gactgattca cagatttcat ccggtgcgat cagtagtgag gcggtcacgg
     8101 tcaacacgtc gaaccctgac ggcttcgacc tgctggctcc gttgccgaac aatctggacg
     8161 ggcgcagcgg aagcgtggcg accaccacga ccacgactga cccggtctat tcaccagccg
     8221 aacccgattc gacgggtacg ggaaccgtca ccgatcagaa gactgccgtt tacaccgacg
     8281 gcacttcgat gacacagccg tcaaccatgc agtcaaccga tgggacggcg atgatcatca
     8341 ccgagtcgcc gagcctcgtg attgacccga tgttctcgac gagcacgctc cgcaagccgc
     8401 caagcacctc gctggcgagc gtctgccccg tgggcgcgcc tccgcgcacc tacaacgtga
     8461 gcgcgtatct ggccagttcc atcgtgccgg gcggcgcgct cgtctacaac cagcgttttg
     8521 gcatcaagga ctcgaaggcc atcattttcg tcgagaactc cgacgtggcc tcgattcaga
     8581 acggaacccg caggccggag cctctcatcc tgagagccgc tgccggcgag tgcatctctg
     8641 tcaccttgac caacaagctg cccagcgtcg tgcccgagat caacagttgg aacatgatgc
     8701 cgatgatcgt caatcgcttc aatttcaatc aggtcaaaac ttcaaaccgc gtgagtctcc
     8761 acccgcaact cgtatcggcc aacacctaca aggacgatgc ttcggcggtg ggcttcaatc
     8821 aggactcgac cgtcgggccg ggtcagacga gaacctacac ttggtacgcg ggcaaccgcg
     8881 tggtcaactc gctcggtaac tacgtgcagg ctcccgtaga atttggagcc acgaacctcc
     8941 gcgatatggg cgacgtgatc aagcacagct cccatggagc catcggctcg ctcattatcg
     9001 agccggttgg cgcgagatgg tcttacccgg ttgccaatag caaagcgacg gcagatgtcg
     9061 ccagtccgac aggcacgttc cgtgagttcg tcgtgattta ccaggacgat ctgaccgtgc
     9121 agaaaaacgg cgtcccgctc ccgaacatcg gggaaacgga cgactccgag gacagcggca
     9181 tgaaggcttt caactatcgc accgaaccgc tctggggacg cctcggcttc tctatcaagg
     9241 agggcgatac gaataacttc gactacaaag acgctttgag ttcgacggca tcgctccccg
     9301 gctgcggcgg agtctgcggc gatcccgaaa cgcctgtctt caccgcgacg gcgggcgtgc
     9361 ccgtgcgctt ccgcgtcctt aatgccgccg gacacccgcg acagcacgcc ttcgcgttgt
     9421 tcggccatca ctggaacttc tcgccattta cgcaactctc gaccgtgcag ggtttcaatc
     9481 ctttcacttt cgaggttggg gcttatgacg gcatcggccc gacgcgccac ttgaacatcc
     9541 tgaccgccgc cggcggactg agaagggtag caggtgatta cctctaccgg actatggaca
     9601 gtcctaactt cgcaggcggc ggactctggg gcattttccg cgtcgtgaaa gcggtaagga
     9661 tcttgactcc catcgaatct gatcctgtcc tctacactga gcctcaacct gctccggcag
     9721 tgattgagaa tcaataactc ttagcagcgc gcgagcctga aacgggttca ggctcgcgcc
     9781 ttcaccttca agcggggacg gaagcgtccc cgcttgaagt aacttttccg caattattca
     9841 ttatccagtt aacccttgat caggccatcc cggcatcgtc atgaaagcaa aaaggacgtt
     9901 tagcatgtac atcattttta atgcactcaa tcgagtaaga ctttccccca acgttccgat
     9961 gcgcgtcgca aaactggcgg caagtgtgct gctgcttgcg gcttcgacgg cgcacgcaca
    10021 aattggcggt ccccatgacg cagcgcgcgc cgccgccaaa aaggctcccg tgaaagcgcc
    10081 cgcttccgtc tcgcaacgct ttgaaaggga aggagtcgcg gttgatttca gcctgaccgc
    10141 ctcgcccgac gcgcaagggc aaaaccccgg actcgtcgcc ggtagcgatg cgaccgtaac
    10201 tttccgcctc agcgatgcgc gcaccggcgc acccgtcacg ggtctgcacc cgactggctg
    10261 gatcagttcc agcacctccc cacacgcgcc caacgaagcg caatgcaaag accgtatcaa
    10321 gacgctggcg ggcgggctgc tgagcacccg cgccgaaata gacctcaaca gctacgtcgt
    10381 cctgaccatc aatcacgaca acacgatctc tttcatcaac ccgttagtct ctttcagcaa
    10441 aacgaaactg gaaaatctga tctcgctgcc gggcgcgggc gcggactggg cgctcacgcg
    10501 acagaaagat ttactctatg tcactttgcc ggagcaatcg gcggtggccg taatcgacac
    10561 cgcgacgcgc aagctcaagg gacaggtggc gaccggtgag aagaccaggc cggtgcgcgt
    10621 tcagcttcag ccggacggtc gctatctctg ggtcggattg gacggctctt ctaagatcgc
    10681 tgtgattgat gctcaagctg ccaggctcgt caccaccctt acggtgggcg aagggctgca
    10741 caacataact ttcaccgccg atagcagctt tgcctacgtt acgaattccg cctcagacac
    10801 cgtctccgtc atcgagacgc aaagattcgt caaggttgct gacatcaagg tcgctaaaac
    10861 tccggtcgcc gttgcctata gcagcgcgag caagctggtg tacgtcgcct cggtcaacgg
    10921 cgagacgatc agcgtgatcg atccggcgaa acagcaggcc gtagccaacg tacaggtcgg
    10981 gcgcggcgtg gtcgcgctca gattcgagcc gaagggtcgc tacggtttcg ccgtcaatca
    11041 ggtcgggagc acagtctccg tgatcgacgc ggccaccaac agcctcgtcg gcacaactgc
    11101 cgtcgttaaa gaaccggatc aggtggtctt tagcgatgaa tatgcttatg tgcgcggcct
    11161 cggttcggag aagttttcgc tgatcgagtt gcgcgaagtg ccctccggca aaatctcgcc
    11221 catctatatt caggcagggc agcgtcccgc gacggatgtg cccaaagaga tcggcgtcgc
    11281 ggacatgatc gcgcccgtgc cgggaggcga cggcgcgatg atcgccaaca ccgccgatgc
    11341 gatgttctat tactacaccg agggcatgat ggctccgatg ggcagcttca ccaactacaa
    11401 gcggcgaccg cgcgccctgc tcatcctcga ccgcagcctc tcggaagtca cgcccggcgt
    11461 ttactcgacc ccgatcaagc tcacttcggc gggtcgcttc gacgtgccgt tcatactcga
    11521 ccagccgcgc atcatcaact gtttccagtt gagcgttgcc gagtcgaacg acaaggcggt
    11581 gaagcagcag gtggccggga gtcttcttat cgaaccgctc tttaaagggg cgcagttcaa
    11641 catgggcgag cgcgtcaaac tccgcttcaa gatcaccgac acggacacgc gccagcccct
    11701 cgccaaacta aaggacgtgc gcgtgctggt cttcgagccg cccggcgtct ggcagcaacg
    11761 ccagtgggcg aaggaagtcg gcaacggcgt ctatgaggtc gagcaggagt ttccgaacga
    11821 cggcgtcttc aacgtgatgg tttccatctt ctcgcgtgga atagagttca gcgatctgcc
    11881 gtttaccgtc gtcgcggtcg aggaaaagaa ggcgtccggt caggcgaaag tcaacgccaa
    11941 ttgatgaagg aaagaattga catgaggaga aatatgaaat ccacgcgcgc actcgtcgcc
    12001 ctgttcttcg tcgcatttat ctgcgcggca gggacggaga gcgtgagcgc gcatgcctca
    12061 caaaaagggc gtgcgcgggc tacgcgcacg ggtgtcgagt acacgtgccc gatgcacccg
    12121 gaagttaaag cgaaacgccc gggcacctgc ccgaagtgca ggatggactt gcgccgcgtg
    12181 agcgtcgctc cgcaggccgc agtcccggcg gacaaccgca acagcgcgac cccggagcgc
    12241 gccggcagca gcagcaacag tagtgcgacg ccggaagccg ggctacgcat ccccgacacg
    12301 aacgtgctcg atcaagacgg gcggcgtttg aacttctaca gcgacctggt caaagggaag
    12361 accgtcgcca ttgattttat ctttacgacc tgcaacggcg tctgcccgac gatgacggca
    12421 aaatttcgcc agttgcagca ggcgttgggt gatcgagtgg ggcgtgacat tcaactgatc
    12481 tcgatcagtg ttgacccgac caccgacgtg cccgcacgcc tccatgctta tgccgcgaaa
    12541 tttcatgccg gacccggctg gactttcgtg accggcgaca agccggaaat tgacgctttg
    12601 ctgcgcgccc tcggcgcgtt tgctcccgac aagacaaagc acccgcagac catcctcatc
    12661 ggtaatgagg ccgctaacta ctggacacgt acgctgggt
//
LOCUS       DASWNS010000159        26218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_58209, whole genome shotgun sequence.
ACCESSION   DASWNS010000159 DASWNS010000000
VERSION     DASWNS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 26218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26218
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58209"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..2773
                     /locus_tag="VGB76_05440"
     CDS             56..2773
                     /locus_tag="VGB76_05440"
                     /inference="COORDINATES: protein motif:HMM:NF040129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEY0098380.1"
                     /translation="MVKKLDLYKLGVVVEVNGQKSLATLQQVEKQSNSVQTSFKGLRG
                     EIGSLKASLTGGGSGGDGGGLFGAISSGLQLLPGLNILAAGVGKVTDVMKSGVKIGFD
                     YNRVMEEAQIGFEVMMKSRTKATQHLKQLDGLTLKSPFDLEVLIKGSELAQAFRFKTA
                     EIQRLLLTTGDASRGVQEKFEGIITALGQIKTKGKLSFEEMNQLVERGVPAWDYLADE
                     IAKVDKNIARLTGDKRTGAVQKMAERGLISSSGAIEALLRGMEADFGGIGERVSNETA
                     SGLESQMRDATRRVVGAGTKGLYGGYKELLRTGIATVSSDTVTGLANSLGGNSERLIG
                     AGVGIAKNLFQGFTDYMEIQSPSRRMARAGADTVEGLENGLVDRTSRGFDRWSKALAN
                     AGGEAFIKAVEEISARLGIDPAWMLNVMAFESGISAKAANKGSSARGLIQFMNRTARG
                     LGLNNSNQFLGMDAMQQLRYVERYFKPYAGKMQNQGDVYAAVAGPAALFRRDRVLYRR
                     GSKEYNANRVWDVNRDGVIVANEIGGLAASRGQFKSVGGSSFAGGAVASFKAGTPVFV
                     TNLDEVRGIAARDLSEHGGSVPRDAASIAAGLDVVAKLRNANADIPLTTNGDIGGFEN
                     VVIKPLQMLPPAMREVGQAAKASLGPIKGLIQAESRLSQLRGAMGQVAGMMPQQQVGK
                     KRGFFSKMLGFAAPFLGFIPGVGPLLSTLAGIGSSALAGDWGGALMATAGGFASGGAF
                     RGSGSTVSPAGNTTNIGGINLPNPGTAIGPVTTIGTTINTNLSNRYPARARGGPVTRG
                     RAYLVGEHHPEVVQFGDDGYVHPSIEAFSRAANSGGGLAGSGKGGFWNSWREHFDRLA
                     GAIEKFETMSPGEVLTKGAKTHPGAIGDGLMRAGARDPQVVDWMRRRSA"
     gene            2770..3231
                     /locus_tag="VGB76_05445"
     CDS             2770..3231
                     /locus_tag="VGB76_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098381.1"
                     /translation="MSDAQIETLITEGFLFEGTSIRGFNLRGNYGDGYGEAVVIGSPT
                     GLRKWKFKVDVLPHFDDYLINAGERGFQTRAQYLWEFYIRHNVANAFKPFWVRDPFLK
                     QDFLAEIVEEELDYMMLCSIAYSTGLTIRQRRVHGVESPSAPITMENNDSI"
     gene            3242..3679
                     /locus_tag="VGB76_05450"
     CDS             3242..3679
                     /locus_tag="VGB76_05450"
                     /inference="COORDINATES: protein motif:HMM:NF040141.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5675 family protein"
                     /protein_id="HEY0098382.1"
                     /translation="MELRLARTALTGRSTIGELSIDGVFECYTLEDCVRPVKIKNVTA
                     IPAGRYRVIINQSQRFKRLLPLLLDVPQFEGVRIHSGNAAGDTEGCILVGQTKAKDFI
                     GNSRVAFDKLFTRLLAAVATEEIFINIKDVPSAVTPPARPHSK"
     gene            3683..5875
                     /locus_tag="VGB76_05455"
     CDS             3683..5875
                     /locus_tag="VGB76_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098383.1"
                     /translation="MPELSALDQAKLEALTGLIAAARSGGESVQVVELAAADWPVPDV
                     RVFYASTFADDVFPGLRAHLGDAIVEPRLQGGQFLDVTRDSGISDDSVALNLWDGDHA
                     ISDLFETHGEGVRFEIFFYFPQVDLLLSMWFGHLRPPEDADEEMFNARAENGFLSVML
                     PLPRRAFFNTCQAVYGGLLATQEEIDEHDCPVNLHIGGSVGLVDPETGLPFPTCPRNS
                     RAACIARLGHDRFYLAFDTKALTYAVGSRGATATTRGNENNLKRPLRVIAGERTVRDL
                     DLLAYIVEIGNPSHPERGAIKLQYAVGEGVFDYLGSPKVNNTLIQPAHWGVRLGWPGQ
                     PGTGFTPGANNYSGTGILQVVLQGDFTNVDPAGIPTEIKARGKNDVRVYSTETTFTPA
                     YTRSRAWWLLEALRNKRWGYGAAVPRFAIESDFIPLDAWFAESVGMRDKNNNVIAGPR
                     TLFDAELIDRTTQQQIKDICLAGRCTVPFPYQGKLRVFPLKKLSEEELAAAPVFADYG
                     DARNIIRDETTGKSSLTRTILSDAELPNSIKVTFDDAEQDHAEHPPLVFDNPEQQLRA
                     GRAFSDTGRRVVEKDYKLLGVTRIGEANRLGVLLRDLGEFDEGGLENNLRVRFQTFFT
                     QALSLYKSKVIRVLSKSLVNRRTGVQKFEYFRVRSVRQMPNLLVEVSAQAYPVEYYER
                     LEDLETPPEGGGGSDLTGPLRGRRPRIVEIKDIDFTNDLITIDIGRTV"
     gene            5879..6121
                     /locus_tag="VGB76_05460"
     CDS             5879..6121
                     /locus_tag="VGB76_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098384.1"
                     /translation="MSQFRTFEIYFDNDDGTVSDVANQTVHVYDVTNGVARDDLVSDA
                     NGTVAGGALAVDAGTLIRFAVVRADGCSRCAEQETI"
     gene            6124..7059
                     /locus_tag="VGB76_05465"
     CDS             6124..7059
                     /locus_tag="VGB76_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098385.1"
                     /translation="MPSAYLAFEVYIDNGDNSTTPVASHVVKVYNASLGVALADLATD
                     ASGHVSGGTLNVAAGTLIRFSVVLDDFNCGYAEAVTDSGHSMDVILKPSGGANIVLRP
                     TALPTWYPEAEVDPVEVELFKQELDSDNKFVGAPESLGRFPSVGRISIRNNPVTDVLV
                     RFYLIAYAGDDTPDVSSLADALQVTVPVKRETDAPTVTLIGAATNTLIELSVTGYTKF
                     AAKRKLRASPNADMSGATETITDYSGHEMPSVVPLTRPAAAGVETIYVAISHSSGGAF
                     GAESAPQGFTFTDAGGSGGSTGGGGGYWKTDPYDL"
     gene            7118..8584
                     /locus_tag="VGB76_05470"
     CDS             7118..8584
                     /locus_tag="VGB76_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098386.1"
                     /translation="MAFNTGAQTIQTTEVSGYIFNGDGTVAAHRPLRIVKVEKNGALI
                     STSTISINSNAAGRVVFNVVRASNVWIEGDVGRFKVPGGLKLTIPDEATASLETLPTV
                     EAIPATGLTAKSNNVLLGGRFGTIDLSSQFALTENPAGELNVTLAQSYTLASELTAHA
                     AASDPHPVYLTQTEGNGLYAALGSGVTAHGLLTGLSDDDHPQYQLRAEKNTANGYVGL
                     DAGALVPDNRLPASIARDTEIATAISALSTVYAALSHTHVAADITNFNAAVLSAIGFT
                     PLDAAATTYALLTGRAGGQTVNGGTGSGENLTLNSTSHSMKGYILLGSGGFVGINRSV
                     PGAQLHVVAGSASTVATIFDTAASPSVDIAQFKNNGTTVLKVSSTGTLLPKGFIDGDI
                     GSDAAPFYLIRGHVLKAGAQLVVNSRFLLDSGGFLTVSDNGSTANGKVNAAEYRVGGT
                     KVLGTQCAAIANATDAATAITQLNSLLACLRTHGSVAP"
     gene            8606..8956
                     /locus_tag="VGB76_05475"
     CDS             8606..8956
                     /locus_tag="VGB76_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098387.1"
                     /translation="MKKLLSLLLISLLLVAFSVSSFTQTPTDVTLKITLPAAQSARLL
                     NAFTKQYGWTAQIPDPANPGQAVPNPVSKRDFAEAVLESFVLQSVEAAEKNEAAEKAR
                     REAATDVKAIKLRP"
     gene            8999..11434
                     /locus_tag="VGB76_05480"
     CDS             8999..11434
                     /locus_tag="VGB76_05480"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0098388.1"
                     /translation="MHRRTHIITITLALIFALTSLVGGYPATITLNTARAATNAPLPF
                     TVPVASSFSATVATNRQNAIQLQGSDAEGTALTYATTSTPSHGSLSNLNSSTGVVVYT
                     PTDGYVGADSFTYTVASGGDTSTAGTVTLAVTNAKTRIIDTIDPSMATGATGITFILT
                     QAATSPGGLIPKGSSVSASVNSLGQFDISVYPSRSLTPAAYYQVWLKTSNLGQVLMGV
                     YDIPLSTTTITLSPYKVTDTNLALQYSFASQAAVAGLVGAAATATTNTLLGTNPTNNA
                     LQKYNSGTGKFTNSTITDNGTAVTVAANTTVSGSVTTTGAVISENIALPAATKAMIAN
                     GQIPYSAEYVKGLVAYFDASTLDLAEGDKVASWPDLSGNARHAVQATGALQPTYHQSE
                     TFTESSAPYVTAGGTTQYLTVPIPASATKSFCLVIRNQTQFPTAYALNFDANNWFSTL
                     APNGSGRWTGYRTSLGGSTTPAGYAQEYSVPCWRMNSASSISVFMDGALVETYTPLDS
                     ALAAATSIKIGPGFGYLRQLAILDYGRAMTDAEVASHSTMLLGHNDLTWEVTYLGRNG
                     VEYAPTLASEGVIVRKRGLRGKRSLVFLNHQASQTERVFFEATEQRAILTALVEAGYI
                     VVISRQWDANALENSWGNDSALDANAELYDYVVAHYPVDTARVAMVGFSMGGISTALA
                     FPDGRIPLKGAACFDCALDLAHLYANGFSSGINAAYQISGGNPYATATSGHDPRLLGA
                     HASFSGKRLAFFRNTDDVIVNPTENTGDFQAQVASVATEETITSYTTGGGHLANIAAS
                     KTLLMEFLARCFA"
     gene            complement(11605..12597)
                     /locus_tag="VGB76_05485"
     CDS             complement(11605..12597)
                     /locus_tag="VGB76_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY0098389.1"
                     /translation="MRLNHRKLFLSFAVSITTLASLAAIVPLGSQPEANRPGLSAEVA
                     EPQEHSAPTPLSKDAETDELRLTEVVSTETDAPPPSLIAGAAQQNRSLKDNLSWTFGG
                     KTQRGWSIYVPLIQRALGTEADATSENFAASLARWQKSVGLKVSGVLDEATLMRMVTT
                     WQSQRPLLKSRVYPRPDQLLTVPPAEFYDSGRADELRQVERQTYAAYKRMLAAAAADA
                     SLQLKTQGGELAASEKYLKIISAFRSREYQDRLRKLEPNAGRAALAVNSPHFTGRALD
                     IYVGGEPVITKDHNRALQTQTPAYKWLVKNAPRFGFCPYFYEPWHWELCSEKVQ"
     gene            complement(12740..13528)
                     /locus_tag="VGB76_05490"
     CDS             complement(12740..13528)
                     /locus_tag="VGB76_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HEY0098390.1"
                     /translation="MNSNFWRTLRAAFNFQEARQKASPLPPPQQQLSKRPVLDRDEAV
                     RRINLLAGRDLRALAGELGVTVWKEGRKNKGWAGHVLERYLGLAQNSRQAPDFGTWDL
                     KLVSLRRTPGGVLRVKETMAITMLEPSEVVSNRFADSHLYDKLRRLVVVSRIFESASD
                     ARSVLHAAAKFDLDDPLIFRAVEEDYELIRATIREKGYAALSGDLGELIQARTKGRGH
                     GSTSRAFYARTAFIARMLNLHKLASMPRVFASPDDAKESAELAD"
     gene            complement(13488..14324)
                     /locus_tag="VGB76_05495"
     CDS             complement(13488..14324)
                     /locus_tag="VGB76_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY0098391.1"
                     /translation="MTREAKTSRPAHKSSRKPFFQNRRHALSLHRGDALELLRAAASE
                     SFDLIFADPPYFLSNNGVTCQSGKRASVNKGTWDKAPAVDDIQAFNVAWLGECKRLLR
                     PHGAIWVTGTAHNIYSVGFALQTLGYKILNDIAWYKVNPPPNLACRYFTHATETILWA
                     KRDPHAKHTFNYGLMKQANGGKQMQSLWQIKSPTTREKRYGKHPTQKPEALLERIIRA
                     SSNEGDSVLDPFCGSGTTGVVAARLKRRFTGFDVDENYLRIASQRITDELQLLANTPR
                     SL"
     gene            14516..16243
                     /locus_tag="VGB76_05500"
     CDS             14516..16243
                     /locus_tag="VGB76_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0098392.1"
                     /translation="MFKSILIRALCVSLLCVCGAAAAVCRAQSLERELPAGAGVSVSV
                     KNLSGRVTIVAAAEGEERKSVSLKAESPGAAVQEADVVSKGAGGQLEVEVREGRAERD
                     RIDLTLRVPQRARVKVETRAGAVDVSGPVSEAEAATDTGTIRADVPLDSLRYSFRWTA
                     SRPRFYSEVELGKVEEKRGGRFEVSGRFGDKKAKREQRVKLDFETERGVILFGVDPAS
                     VPADLRERQLTEAARAIIRSGNQDLIEAIRKVTPRFVGEYAKTLPPPRGGAPVLSVVA
                     KGERAAAREVAPQLMRVNANVTDRLGRAIAGLERKDFSVLEGSEPREVVSVEPTRTPF
                     NLVLLLDVSGSVEERLDFIRKSALAFANTVSPQDRIAIISFRDDIQLISDFTTDRRLL
                     AQRIKDIEAGGGTALYDALAYTLVNTLKPLRDERTAIVVISDGDDNRSFIPFPYILET
                     IIETGAVVYPLYIPSGLIPASGAPAPLTTLDPTRTRFLSLTSRADEEGRKLASVSGGV
                     YYPVRRFDELQLAYDDIVAQLRTSYTVSYATTSAATATRNRVRVRVAREGAVVRLSPV
                     VGSGTSQPN"
     gene            16326..17732
                     /gene="xseA"
                     /locus_tag="VGB76_05505"
     CDS             16326..17732
                     /gene="xseA"
                     /locus_tag="VGB76_05505"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY0098393.1"
                     /translation="MKSSLLLSLFDETERRPLSVSELTSQVRGALENRFASVWVEGEI
                     SNFKAASSGHWYFTIKDESAQLHAKCFRGVNGRIRFRPSDGLQVRARGRITVYEPRGE
                     YELLVEGLDPVGAGALRVAFEQMRDRLAAEGLFAEELKRPLPLLPRRVGVVTSPTGAA
                     IRDIINVIKRRTRTVNILVAPTRVQGVGAGAEIARAIRSLNEHHRRAQREGRVRDYID
                     AIIVGRGGGSSEDLWAFNEEEVARAIRASAIPVISAVGHETDFTIADFVADVRAPTPS
                     AAAEIVAAREDEMTAIVATLTRDLVRAVEYRLLIERERVHEAASSESFDEVRERLRAA
                     GRMTDELARRLESSTLRAHERARKRLESVVQRLSPARAAARTSEGNVRLALARAALDA
                     NFGARLDERRARLEVAVASLDALSPLGVLARGYALTQDARGRVLRSSRSVRAGERVRV
                     RLAEGSLQCRVEEVEGES"
     gene            17732..18502
                     /locus_tag="VGB76_05510"
     CDS             17732..18502
                     /locus_tag="VGB76_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098394.1"
                     /translation="MSTVPGERAKKGKRSRIVVNVEPKPPEERGGAVGRWRGKSGARR
                     TGGRRRRALLVAAIPFVVIALLFFGVGYWWWQSFKSSPAYTLALVVDAAERDDRETFE
                     RLVDVEGVSRSVVPQVIERVRGGDNALNIAPQVRRQIAQNAQVWLPGVRQQVGDVLMA
                     ETKGLAERGGARNYPFFVRAFALSRLGESQTQEGTAGRVPTASVTYQVNGRPVEFGLL
                     GGDGSEGQPDWKIVSLKSDDLATRVAEQVAKIFPMTGR"
     gene            18517..18723
                     /gene="xseB"
                     /locus_tag="VGB76_05515"
                     /pseudo
     CDS             18517..18723
                     /gene="xseB"
                     /locus_tag="VGB76_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            18803..19750
                     /locus_tag="VGB76_05520"
     CDS             18803..19750
                     /locus_tag="VGB76_05520"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY0098395.1"
                     /translation="MKPPSDALIETAGAHETASAEEAHAYLAAVRTLVDASLELLVPP
                     GTHEPRAVHAAMRWSLFAGGKRLRPALALASGEAFGAPPLRLTRTACAFELIHTYSLI
                     HDDLPAMDDDDLRRGRPTCHIRFGEATAILAGDAMQTLAFQTIAEDSELDAHVRVRLV
                     AELARAAGTPAGMVAGQAHDLAGESRTDVTADELDSIHRHKTGALIVAAARAGAIVAQ
                     ASEREIERISDYAAALGLLFQITDDLLDVTATAETLGKTPGKDARAQKATYPALHGLE
                     ATQERALAVYTQALDALAQIERPTRLLRSIARLILERRT"
     gene            19920..22940
                     /locus_tag="VGB76_05525"
     CDS             19920..22940
                     /locus_tag="VGB76_05525"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEY0098396.1"
                     /translation="MSYTKRLILSLLVAVSASLALSVCLARAYSNETRPETQDATLAV
                     VAAAPEKFGAIPSTTPIIGQTRLVFTSDRDGNAEVYSQAGDNSPQVNLTNNPATDACP
                     VISPDGTKIAFTSNRNGQSDIYVMNTDGSNVQAVTHFGDGGGVNTRAYDPAWSPDGTK
                     LAYVASFFGGESSGLFVVNADGSGEAAHIGNGSEAADPAWSPDGTRIAYVGREGSYFP
                     TEEGFPYFLFVTNADGSGTTRIATDTPIFSSFAYPPEASGPSWSRDGARLAFVSNRHG
                     NEEIYTVSATGGAVMRLTNNPATDTLPTWLGDGIAFTSTRGGARDVYVMYPGSPLISG
                     EPLMFRITGNAGNNFDADWRSLTPAAPSTISASRIAFIRANPANPDDTDIYTSNPDGT
                     NEVNITNSPQKEFAPAWSPDGKMIAYVRRPERALFVMNADGTNARELTPVSDVAVKPS
                     WSPDGRRIAFIGGSDLAYTLAVVNVDGTERRRLVPLDATYHEIAWSPDGTRLAYVLDR
                     ASAFADPAIHVVNLDGTGARMLSTGINRDRQPAWSPDSSKLAFTSTRDANVEIYSMNA
                     DGTNQTRLTNNPATDASPIWSPDGQTIIFSTNRDGDFELYRMNAADGGGQARITDTSA
                     NEVAPDWNRGALSTIQWRHPSFRIREGLEHGSSGEPLVVTRLGDLTHPATVEYATTHG
                     CRDAFDAPPSMCGSGFASERSDYVSTTGTLRFAAGEASKTISVTTIDDAIIEGDERFN
                     LVLTNATGATLGEQRDVPVIITDNDKNAAAPNPIDEPRFFVRMHYLDFLGREPEQRGW
                     DDWVGVWTRCPNIFNNPVCDRVTISASFFRSREFYVKSYYIYLFYRAALGRRPTYAEF
                     VRDLPRLAAQTGAELDARKDDYNLAWIHRAEFAEVARTFTHEQFVDRLVQNVGITLTG
                     AVTRETLLADLRAGRRTRAAILRAVVEHPDVERAEYNGAFVTMQYFGYLKRDPDATGF
                     ANWLNYLNAHPTDFRTMVHGFVVSNEYRARFGKP"
     gene            complement(23120..23929)
                     /locus_tag="VGB76_05530"
     CDS             complement(23120..23929)
                     /locus_tag="VGB76_05530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0098397.1"
                     /translation="MKTFVIGDVHGRRAQLNHLLEIIPRDASHDMLILLGDIIDRGDD
                     VPGTVADVVKLKDEQPERVLCLRGNHEQMLLDFVDESATLWLHAAVGSECTFRQYTGK
                     PLHVRSEQDFTTLRQDFTESLPPAHLDFFRTLPLFHEDDYALYVHAGLSKGQHPRDTP
                     PQHLLWSRDSDFFRNYTGKPCVFGHTPAPLLPLRGRLGRHGIYISHSAIGIDSGYINS
                     SPLSCLQLPDCMLYQAFADGHTATHHLTAFMPETLRALQRKVATHQPTTGV"
     gene            complement(23993..>26218)
                     /locus_tag="VGB76_05535"
     CDS             complement(23993..>26218)
                     /locus_tag="VGB76_05535"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0098398.1"
                     /translation="RPLELRSAISITMQVADALAAAHARGVIHRDIKAGNVMVTKNGT
                     AKVLDFGLAKLLDENKSGGKGMEQHLTEMGVPYGTATYAAPEQATGAHVDHRADIFST
                     GVLLYEMLAGIWPFQGKTNVEVRYAVLHDTPVRVGEFRRDAPPRLQEILDRALAKDPK
                     DRYQRVAEFRDDLRAVKRELAAVGAAAGGGNSIDTTDEFEPVAPRHQSSNSSFGRTFR
                     RWLRNITGTETPTSAQSTSAPPNASASRNISAAPHRSEGEPQHGTNLSESAQKSIAIL
                     PFRNLSGDAEVSFYEFSLADAVITELARLRSLVVRPSSLIVKYQGQERDPREIGEELS
                     VGAVLAASFLRAGDKLRVNAQLLDVASGDLLWTDRIDATAADIIAVQDTIAQRIVEGL
                     RVELSADEKSGLAQRSTANPEAYNIYLRGRDAMGRYIYHTLANEDVEEAIEHFKRAIE
                     LDPNFALAHCALGGCYANRVIKSMGDFEDYERAEEAFRKGLDIDPKIMEARMHMVFIY
                     LARGEKSKARAAVENLRHEAPNDVGVQFVASYLYRLDGDYERSLRSLDRMLALNPAER
                     VVVSYNRARVLMYQGRMDEAMLALDDGARIEPDHPFVKTFRAVAFYRQGNAAGAAEIL
                     RDVLDTHPEMDGIRPHYAIALSKLGQHEAARAQLTERVKEVAEADHDAPYWIATAYAV
                     EGMRDQAFKWLERAINRGNENLPWFRANPEWTELRADPRFETLMERIEKSHAQARVGV
                     E"
BASE COUNT         4925 a         8223 c         8306 g         4764 t
ORIGIN      
        1 aaccgcatgt tgatggacat ggaggcgcgc gctaaagccg ccgagaagtg aggcaatggt
       61 caagaaactc gacctgtata agctgggggt agtcgttgaa gtcaacgggc agaaatcgct
      121 cgccacgctt cagcaggtcg agaagcaatc gaactccgtc cagacctcgt tcaaaggtct
      181 gcgaggtgag atcggctcac tgaaggcatc tctaaccggc ggcggaagcg gaggcgatgg
      241 tggcgggctg ttcggcgcca tctcgtcggg gctgcaactg ctgcccggcc tcaatattct
      301 ggctgcaggc gtcggcaaag taaccgacgt catgaagtcc ggcgtcaaga tcggattcga
      361 ctacaaccgc gtcatggaag aagcccagat cggctttgaa gtgatgatga agagtcggac
      421 gaaagcaacg cagcacctta aacaactcga cggcctcacc ctcaaatccc ccttcgattt
      481 ggaagtcctc atcaagggct cggaactggc gcaggctttc cggttcaaga ctgcggagat
      541 ccaacgcctc ttacttacca ccggcgacgc atcgcgcggc gtgcaggaaa agttcgaggg
      601 catcatcacc gcgctcggcc agatcaagac caaaggcaag ctgagttttg aagaaatgaa
      661 ccagcttgtc gagcggggtg tgcccgcttg ggattacctc gccgacgaga tcgccaaagt
      721 tgataagaac atcgcccggc tgaccggaga taagcgcacc ggcgccgtgc aaaagatggc
      781 ggagcgcggg ttgatctcca gctccggcgc tatcgaggcg ctcctgcgcg gaatggaagc
      841 cgacttcggc ggcatcggcg agcgggtttc gaatgaaacc gcctccggcc ttgagtcgca
      901 gatgcgcgat gccactcgcc gcgtggtcgg tgccggaact aagggtcttt acgggggcta
      961 caaggagttg cttcggacgg gcattgcgac cgtcagttcc gacaccgtca caggcttggc
     1021 caattcgctc ggcggcaact ccgaacggct catcggcgca ggcgtcggga tagcgaagaa
     1081 cctcttccag ggcttcaccg attacatgga aatccagtcc ccgtcgcgcc ggatggcgcg
     1141 cgccggcgcg gacacggtcg aggggctgga gaatggactg gtagatcgca ccagccgcgg
     1201 cttcgaccgt tggtcgaagg cgttggccaa cgccggcggg gaggcgttca ttaaagccgt
     1261 cgaggagatc tccgcgcgcc tcggcatcga tcccgcgtgg atgctgaacg tgatggcgtt
     1321 cgagtcgggc atcagcgcga aagcggcgaa caagggatca tcggcccgcg gcctcatcca
     1381 gttcatgaac aggactgcca gggggttggg cttgaacaat tcgaaccagt tcctcggcat
     1441 ggacgcgatg cagcagctcc ggtatgtcga gcgctacttc aaaccttacg ccgggaagat
     1501 gcagaaccag ggcgacgtgt atgccgccgt cgccggcccg gcggctctct ttcgtcgtga
     1561 tcgtgtcctc taccggcgcg ggtcgaaaga gtacaacgca aaccgcgtct gggacgtgaa
     1621 ccgcgacggc gtcatcgtcg ccaacgagat cggcggcctc gcagccagtc ggggccaatt
     1681 caagagcgtg ggtggaagct cattcgccgg aggcgcggtc gcgagtttca aagcagggac
     1741 gccggtcttc gtcacaaacc tcgatgaagt gcgcggcatc gccgcacgcg atctgagcga
     1801 gcacggcggc tccgtcccgc gcgatgccgc ttccatagcg gcggggctcg acgtcgtggc
     1861 gaagctgaga aacgctaatg cggacatccc cctgacgact aacggtgaca tcggcggatt
     1921 tgaaaacgtc gtgatcaagc cgctccagat gttgccgcct gcgatgcgtg aggtgggcca
     1981 agccgcgaaa gcatctctcg gcccgatcaa aggtttaatt caggcggaat cccgcctcag
     2041 ccaactgcgc ggcgcgatgg gccaggtcgc gggcatgatg ccgcagcagc aggtcgggaa
     2101 gaaacgggga tttttctcga agatgctcgg cttcgccgcg ccgttcctgg gcttcatccc
     2161 aggcgtcggc ccgctcctct cgacgctcgc gggaataggc agcagcgcac ttgccggcga
     2221 ctggggcggg gcactgatgg ccaccgcggg cggattcgcc tctgggggcg cgttccgcgg
     2281 cagcggcagc acggtcagcc cggcgggaaa tacgaccaac atcggcggca tcaacctccc
     2341 gaaccccggc accgccattg gcccggtcac gaccatcggt accaccatca acaccaactt
     2401 gagcaaccgg tacccggccc gcgcacgagg cggccccgta actcgcgggc gcgcgtacct
     2461 cgttggtgag catcacccgg aggtcgtcca gttcggagac gacggctatg tccacccgag
     2521 cattgaggct ttctcgcgcg ccgcgaattc gggtggcggg ttagccggca gcgggaaggg
     2581 cggattctgg aatagctggc gcgaacactt cgaccgccta gctggcgcca tcgagaaatt
     2641 tgaaacgatg tcacccggag aggtcttgac gaagggcgcg aagacgcacc cgggtgccat
     2701 cggcgatggc ctgatgcgtg ccggcgcgcg cgacccccaa gtagttgact ggatgagacg
     2761 gaggtcagca tgagcgacgc acagatcgaa acgctcatta cagaaggctt cctcttcgag
     2821 gggacgtcca ttcgcggctt caacctgcgc gggaactacg gcgacggcta cggcgaggct
     2881 gtagtcatcg gctcgcccac aggactgcgc aagtggaaat tcaaagtcga tgtactcccg
     2941 cacttcgacg attacttgat caacgcgggg gagcgtggct tccagacacg tgctcaatac
     3001 ctttgggagt tttacatccg ccataacgtc gccaacgcct tcaagccttt ctgggtgcgc
     3061 gaccctttcc tcaagcaaga cttcctcgcg gagatcgtgg aagaggaact tgattacatg
     3121 atgctgtgca gcatcgccta ctcgaccggc ctgacgatcc ggcagcggcg cgtgcacggc
     3181 gtggagtcac cgagcgcccc tatcacgatg gagaacaacg acagtatctg aggtgtgaat
     3241 tatggaatta cgactcgcgc gcacagcttt gacgggtaga tcaactatcg gcgaactgag
     3301 catcgacggc gtgttcgagt gctacacgct cgaagactgc gtacgccccg tgaagattaa
     3361 gaacgtcacg gcgatccccg ccggcaggta ccgggtgatc atcaaccagt cgcaacgctt
     3421 caagcggctg ctgcctcttc tgctggatgt cccgcaattc gagggcgtca ggattcatag
     3481 cggcaatgct gcgggagaca ccgaaggctg catcctcgtc gggcagacga aagccaaaga
     3541 cttcattggc aacagccgcg tcgctttcga caaactcttc acccggctgt tggcggcagt
     3601 ggccaccgaa gagatcttca tcaacatcaa ggacgtgccg tccgctgtga cgccgccggc
     3661 gcgaccgcac tcgaagtaat tcatgccgga actgagcgca ttagaccagg ccaagctcga
     3721 agccctaacg gggcttatcg cagccgcgcg ctctggcgga gagtccgtcc aggttgtcga
     3781 actcgccgcg gcggattggc ctgtgcccga cgttcgtgtc ttctacgcct cgacgtttgc
     3841 cgatgacgtc ttcccgggct tgcgcgctca cctcggcgac gccatcgtcg agccgcgcct
     3901 gcaggggggg cagttcctcg atgtcacgcg cgacagcggc atcagcgacg actccgtcgc
     3961 gctcaattta tgggacggtg atcatgccat ctccgatctc ttcgagacgc atggcgaggg
     4021 cgtgcgcttc gaaatattct tctactttcc gcaggtggac ttgctgctct cgatgtggtt
     4081 cgggcatttg cgaccgcccg aagacgccga cgaggagatg ttcaatgcgc gcgctgagaa
     4141 tggtttcctc tccgtcatgc tgccgcttcc tcgacgcgcc ttcttcaaca cgtgccaggc
     4201 cgtgtacggt ggattactcg caactcagga ggagattgac gagcacgact gcccggtgaa
     4261 tctccacatc ggcggcagcg tcgggctggt cgatcccgaa accgggctgc cgttcccgac
     4321 ctgcccgcgc aactcacgcg cagcctgcat tgcgcgtctc ggccatgatc gcttctacct
     4381 tgccttcgac acgaaggcat tgacctacgc agtgggatcg cgcggcgcca cggccacaac
     4441 gcgcggcaac gagaacaatc tcaagcgtcc gcttcgggtc atcgccggcg agcgcaccgt
     4501 gcgcgacctc gacctgctcg cctacatcgt cgaaatcggc aacccctcgc acccagaacg
     4561 cggcgcgatc aagctccagt acgccgtagg cgaaggcgtc ttcgattacc tcggctcgcc
     4621 gaaggtgaac aataccctca ttcagccggc gcactggggt gtgcgcttag gctggcctgg
     4681 ccagccgggc actggcttca caccgggcgc gaacaactac agcggcaccg gcatcctgca
     4741 agtcgtcctc cagggcgact tcacgaacgt agacccggcg ggcatcccga ccgagatcaa
     4801 agcgcgcggg aagaacgacg tgcgcgtcta ctcgaccgag acgaccttca ccccggccta
     4861 cacgagatcg cgcgcctggt ggctgctcga agctctcaga aacaagcgct ggggatatgg
     4921 cgcagccgtc ccgcgcttcg ccattgaatc ggacttcatc ccgctcgatg cgtggtttgc
     4981 cgagtcggtc ggcatgcgtg acaaaaacaa taacgtcatc gctggaccgc gcacgctctt
     5041 cgatgccgag ttgattgacc gcacgacaca gcagcagatc aaagacatct gcctcgccgg
     5101 tcgttgcacc gtgcccttcc cctaccaggg caagctccgc gtcttcccgc tcaagaaact
     5161 cagcgaagag gaactcgccg ccgcgcctgt cttcgctgac tacggggacg cgcgcaacat
     5221 catccgtgac gagacgacgg ggaagtcgtc tctcacgcgc accattctct ccgatgccga
     5281 attgccgaac tccatcaagg tcaccttcga cgacgccgag caggaccacg ctgagcaccc
     5341 gccactcgtc ttcgacaacc ctgagcagca actgcgcgct ggccgtgcct tcagcgacac
     5401 tggccgccgc gtcgtcgaga aagattacaa actgctcggc gtcactcgca tcggcgaggc
     5461 caatcgcctc ggcgtgctcc tgcgcgacct gggcgagttc gacgaaggcg gcctggagaa
     5521 caacctgcgc gtcaggttcc agaccttctt cacgcaggcg ctctcgctct acaagtcgaa
     5581 ggtcatccgc gtgctctcga agtctctcgt caaccggcgc accggcgtgc agaagttcga
     5641 gtacttccgc gtgcgcagcg tccggcagat gccgaacctt ctcgtcgaag tgagcgcgca
     5701 ggcttacccg gtcgagtatt acgagcggct ggaagacctt gaaactccgc ccgagggcgg
     5761 cgggggcagc gacctgacgg ggccgctccg gggacgtcgt ccgcgcatcg tcgagatcaa
     5821 agacatcgac ttcaccaacg acctcatcac catcgacatc gggaggactg tgtaagccgt
     5881 gtcgcaattc aggacctttg aaatttattt cgacaacgac gacggcactg tctcggacgt
     5941 ggcgaaccag accgtgcatg tctatgacgt gacgaacggc gtggcgcgcg acgacctggt
     6001 gtcggacgcg aacggcaccg tcgcgggcgg cgcgctggcc gtcgatgccg gcacgctgat
     6061 tcgcttcgcg gtcgtgcgcg cggacgggtg cagccggtgc gcagagcagg agacgatcta
     6121 accatgcctt cggcttacct cgcatttgag gtctacatag acaacggcga caactcgacg
     6181 acgccggtgg cgagccatgt ggtcaaggtc tataacgcgt cgctcggcgt cgccctggct
     6241 gacctggcga cagacgcgag cggccacgtc tcaggcggaa cattgaacgt cgccgccggc
     6301 acgttgattc gtttcagcgt cgtactcgac gatttcaatt gcggttacgc cgaggccgtc
     6361 acggattcag ggcactcgat ggatgtcatc ctcaagccga gcggaggcgc caatatcgtc
     6421 ctgcgcccga ccgcgctccc cacctggtat ccggaggccg aggttgaccc ggtcgaggtg
     6481 gagttgttca aacaggaact cgactccgac aacaaattcg tcggagcgcc ggagtccctc
     6541 ggtcgcttcc ccagcgtcgg gcgtatctcc atacgcaaca acccggtcac ggacgttctt
     6601 gtccgtttct atctcattgc ctacgccggc gacgacaccc ccgacgtctc ctcgctcgcc
     6661 gacgcgttgc aggtaaccgt gccggtgaaa cgggagacgg acgcgccgac ggtgacgctc
     6721 atcggagccg cgaccaacac gctcatcgag ttgtcggtga ccggctacac aaagttcgcg
     6781 gcgaaaagaa agctgcgcgc ctccccgaac gccgacatga gcggagcgac cgagacgatc
     6841 acggattaca gcgggcatga gatgccgtcg gtcgtgcctc ttacccgccc cgctgcagcg
     6901 ggagtcgaga cgatctacgt ggcgatctct cactcctcgg gcggcgcgtt cggcgcggaa
     6961 tcggcgccgc agggcttcac ctttaccgat gccggcggca gcggcggctc aaccggtggc
     7021 ggcggcggtt actggaagac tgacccttac gacctttaag gagttgatta tgtattcaag
     7081 aaatttagca agtctttgcc tggtgataat cctctgtgtg gcattcaaca ccggcgcgca
     7141 gaccattcag acgaccgagg tgagcggtta catcttcaac ggcgacggca cggtggcagc
     7201 ccaccgcccg ctgcgcatcg tcaaggtgga aaagaacggg gcgctcatct ccacctccac
     7261 gatctccatc aactcgaacg ccgccgggcg cgtggtcttc aacgtagtcc gcgcaagtaa
     7321 cgtttggatt gagggagacg ttggacggtt caaagtgccg ggcggcctca aactcacgat
     7381 cccggacgag gccacggcct cgcttgagac gctccccacg gtcgaggcca taccggccac
     7441 cggactcacc gctaaatcga acaacgttct gcttggcggg cgcttcggga cgattgatct
     7501 ctcatcacag ttcgcgctga cggagaatcc ggcgggcgag ttgaatgtca cgcttgcgca
     7561 atcttacacg ctggcgagcg aactgacggc gcacgcggcg gcgagcgacc cgcacccggt
     7621 ttacttgacg cagacggaag gcaacggcct ttacgccgca ctcggcagcg gtgttacggc
     7681 tcacggactg ctgactggcc tctccgacga tgatcaccct cagtatcagt tgcgcgcaga
     7741 gaagaataca gccaacggct acgtaggact cgacgcgggc gcgctcgtgc cggacaaccg
     7801 cttgccagcc agcatcgcgc gtgacaccga gatcgcgacg gccatcagtg cgctctcgac
     7861 ggtgtatgcg gcgttgtcac acacacacgt tgcagcagac atcacgaatt tcaacgcagc
     7921 ggtgctttcg gcgataggct tcacgccgct cgacgcggct gcgaccactt acgcgttgtt
     7981 aacggggcgc gcaggcgggc agaccgtaaa tggcgggact ggttcaggtg agaacctcac
     8041 gctcaattca acctcacact ctatgaaggg ctacatcctg ctcggcagtg gtgggttcgt
     8101 cggtattaac aggagtgttc ccggcgcgca actccacgtc gtagcaggta gcgcgtcaac
     8161 ggtggcgacc atcttcgaca cggcggcgag tccctcagta gatatagcgc agtttaagaa
     8221 taacgggacg acggtgctga aagtctcttc aaccggcact ttgctcccga aaggatttat
     8281 cgacggcgac atcggatcgg atgccgcacc cttctatctc atcaggggtc acgtcttaaa
     8341 ggcaggcgca caactcgtcg tgaactcccg gttcctcctc gactcgggcg ggttcctgac
     8401 cgtctcggat aacgggtcaa ccgctaacgg gaaggtgaac gcggcagagt acagagtcgg
     8461 gggaacgaaa gttctcggca ctcaatgcgc tgccatcgct aacgccaccg acgccgccac
     8521 ggccatcacg caactcaatt cactcctcgc ctgcctgcgc acgcacggct cagtcgctcc
     8581 ctaataactc ggaaaggaca ccaccatgaa gaaattactt tcgctcttac tcatctccct
     8641 cttgctcgtc gccttctcag tctcgtcttt cacgcagact ccgaccgacg tgacgctcaa
     8701 gatcacgctc cccgctgcgc agtccgcccg actcttaaac gccttcacga agcaatacgg
     8761 ctggacggcg cagatacctg acccggccaa ccccggacag gcggttccga accccgtatc
     8821 aaagcgtgac tttgccgagg cggtgctcga atccttcgtg ctccagtcgg tcgaagcggc
     8881 ggagaagaat gaggcggcgg agaaggctcg cagggaggct gcgacggacg tgaaggccat
     8941 caagctcaga ccataagaac gaatgtccgt tcacgattaa caatttccgg aggaagctat
     9001 gcacagacga acacacatca tcaccattac actggcgctc atcttcgcgc tgacgtcgct
     9061 tgtcggcggt taccccgcta ccattaccct caacacggcg cgcgctgcca caaacgcccc
     9121 tctgcccttc actgtgcccg tagcgtcctc attctcggcg accgtggcga ccaaccgaca
     9181 gaatgcgatc cagttacagg ggagtgacgc cgaaggcacg gcgctcacct acgcgaccac
     9241 ctcgacgccg tctcacggct cgctctcgaa tttaaatagt tcgacgggcg tcgtcgtcta
     9301 tacgcccaca gacggttacg tcggcgcaga ctctttcacc tacaccgtgg cgagcggcgg
     9361 cgacacgagt actgccggca cggttacgtt ggccgtgacg aacgcgaaga cgcgcatcat
     9421 cgacaccatc gatccgagca tggcgacggg cgcgaccgga ataactttta tcctgacgca
     9481 agcagctact tccccagggg gactgattcc caaaggctcc tccgtctcgg cttccgtcaa
     9541 tagtctcggg cagttcgaca tttctgttta cccgtcacgc tcgctcacgc ctgccgccta
     9601 ctaccaggtg tggcttaaga ccagcaacct cgggcaagtg ctgatgggcg tctacgacat
     9661 cccgctctcg acgacaacca tcacgctaag cccttacaag gtgaccgaca cgaacctcgc
     9721 cttacaatat tccttcgcct cacaggcggc ggtggcgggg ctggtgggtg ccgcggcgac
     9781 ggcgacgacg aacacgctcc tcggtaccaa cccgaccaac aacgcgctcc agaaatacaa
     9841 ctcggggact ggcaaattca ccaacagcac gatcacggac aacggcactg ccgtgacggt
     9901 cgcggcgaac actacggtca gcggcagcgt gacaacgact ggcgcggtca tttccgagaa
     9961 catagcactg ccggctgcga cgaaggccat gattgccaac gggcagatcc cctactctgc
    10021 agagtatgtc aagggtctgg ttgcgtactt cgacgcgagc acgctcgacc tggcggaggg
    10081 cgacaaagtt gcatcgtggc cggacttgtc aggtaacgct cgccacgccg tgcaggctac
    10141 cggggcgctg caaccgactt atcaccagag cgagacgttc accgagtcga gcgcgcctta
    10201 tgtcacagcg ggcggcacga cgcagtatct gaccgtccct atccctgcga gcgcgaccaa
    10261 gtccttctgc ctggtcatcc ggaatcaaac gcagtttccc acagcctacg cgctcaactt
    10321 cgacgcgaac aactggttct caacgctcgc ccctaatggt tcgggacgct ggacgggcta
    10381 ccggacatcg cttggaggca gcacgacgcc agcaggctac gcgcaggagt acagcgtccc
    10441 ctgctggcgc atgaactccg ccagttctat cagcgtgttc atggacgggg cattggtaga
    10501 gacgtacacg ccgctcgact cggcactcgc cgcggccacc tcaatcaaga tcgggccggg
    10561 cttcggctat ctcaggcagt tggccatctt ggactatggg cgcgcgatga ccgacgcaga
    10621 ggtggcgagt cattcgacca tgctgctcgg gcacaacgac ctgacgtggg aagtcaccta
    10681 cctcggtcgc aacggggtcg agtacgcgcc gacgctcgcc tctgaagggg taatcgtcag
    10741 gaagcgcggc ctgcgcggga aacgctcgct cgtattcctg aatcatcagg cgtcccagac
    10801 agagagagtc ttcttcgaag caacggagca gcgggcgatc ctcaccgcgc tcgtcgaggc
    10861 tggttatatc gtcgtcatca gccggcagtg ggacgcgaac gctttagaga acagttgggg
    10921 caacgactcg gcgttagacg ccaacgctga gttgtacgac tacgtcgtgg cgcattaccc
    10981 ggtggacacg gcgcgcgtgg cgatggtcgg cttctcgatg ggcgggataa gtactgcgct
    11041 cgcgtttccg gacggacgaa tcccactcaa gggcgcggcc tgcttcgact gtgcgctgga
    11101 cttggcgcac ctctacgcga atggcttttc gagcggcatc aacgccgctt atcaaatctc
    11161 cggagggaac ccttacgcga ccgcgacgag cggccacgac ccacgcctgc tcggcgcgca
    11221 cgcatcattt agtggcaagc ggctcgcgtt cttccgcaac accgacgacg tgatcgttaa
    11281 cccaacggaa aacacgggag attttcaggc gcaggtcgcg tctgtggcga ccgaggagac
    11341 gatcacgtcc tacacgaccg ggggcggcca cctcgccaac atcgcggcgt cgaagacttt
    11401 gctgatggaa ttcctcgcgc gctgcttcgc gtaaagatct aatgtcgctg cagccggggc
    11461 agacagcgag gcagcaaaaa agttacggtc accttgatgg tggccgtaac ttattgtaga
    11521 tatcgactcc gcaagtagga ctcaatccta caaaccttcg gttagcagtc aaaatatcgc
    11581 catcaatttc aaaaaatata gattttactg gactttctcg ctacatagtt cccagtgcca
    11641 cggttcgtag aagtatggac agaagccgaa gcgtggggcg tttttgacga gccacttgta
    11701 ggcaggcgtc tgtgtctgga gcgcgcggtt gtggtctttc gtgatgacgg gttcgccgcc
    11761 cacgtagatg tcgagcgcgc gccctgtgaa gtgcgggctg ttgacggcga gcgcggcgcg
    11821 tcccgcattc ggttccaact tgcgcaggcg atcctgatat tcgcgcgaac gaaaggccga
    11881 gatgatcttc aaatacttct ccgatgcggc gagttcgcca ccctgcgtct tgagttgcag
    11941 ggaagcgtct gcggcggcgg cggccagcat gcgtttgtaa gccgcgtaag tttgccgctc
    12001 gacctgacgc agttcgtctg cgcgtcccga atcgtaaaac tctgcgggcg ggacggtgag
    12061 caactggtct ggtcgcgggt agacgcggct tttaagcagc ggacgctgcg actgccacgt
    12121 cgtaaccatc cgcatcaagg tcgcctcgtc gagcacgccg ctcactttga gtcccacgct
    12181 cttttgccag cgcgcgaggc tggcggcgaa attctcgctc gtcgcgtctg cctccgtgcc
    12241 caacgcgcgt tgaatcaacg ggacgtagat cgaccagccg cgctgcgtct tgccgccgaa
    12301 cgtccaactc aagttgtctt tgagcgagcg gttctgttgc gccgctcctg cgatgagaga
    12361 gggcgggggc gcgtccgtct ccgtcgagac gacttctgtg agtcgcagtt catccgtctc
    12421 cgcgtccttt gacagaggcg tgggcgcgga gtgttcctgc ggctccgcca cttctgcgga
    12481 caggccgggg cgattcgctt cgggttgcga gccgagaggc acgatggccg cgagggaagc
    12541 gagcgtcgtg atcgacacgg cgaaggagag aaataatttt ctgtggttga gccgcataaa
    12601 cgtcaatcaa tcatggggag agaggtgcag gctgcttcgc catcttagtg aagcgcaagg
    12661 acgcgactcc gcgaaaacag tttgtcgccg ccgtccgctt caatcgcaca ggggagagat
    12721 gaattttagt tcgccgcgct caatctgcca actccgccga ttccttcgcg tcgtccggcg
    12781 aggcgaagac gcgcggcatc gaagccagct tgtgcaggtt gagcatgcgt gcgatgaatg
    12841 ctgtgcgcgc gtaaaaggcg cgcgaggtgc tgccgtgccc gcgccccttc gtgcgcgcct
    12901 ggatcaactc gccgaggtcg cccgagagcg cggcgtaacc tttctcccga atcgtcgcgc
    12961 ggatcaattc gtaatcttct tccacggcgc gaaagattaa cggatcgtcc agatcaaact
    13021 tcgcggcggc gtgcagcacc gaacgcgcat ccgacgcgct ctcgaagatg cgactgacga
    13081 cgacgagccg ccgcagcttg tcgtagagat ggctgtctgc gaaacgattc gagacgacct
    13141 cggaaggctc aagcatcgtg atcgccatcg tctccttgac gcgcagcacg ccgccgggcg
    13201 tgcgcctgag cgagacgagt ttcaaatccc acgtgccgaa gtccggcgct tgccgcgaat
    13261 tctgcgcgag gccgagatag cgttccagca catgccccgc ccagccctta ttcttgcgcc
    13321 cctccttcca gaccgtcacg ccgagttcac cggccagcgc gcgcaagtct ctcccggcga
    13381 gcagattgat gcgccgcacg gcctcatcgc ggtccagcac gggacgcttt gaaagctgct
    13441 gttgcggcgg cggaagcggc gacgcttttt gtctcgcttc ctgaaagtta aaggctgcgc
    13501 ggagtgttcg ccaaaagttg gagttcatct gtgatgcgtt gtgaggcgat gcgcaggtag
    13561 ttttcgtcaa cgtcgaagcc ggtgaagcga cgtttgagcc gggcggcgac aaccccggtc
    13621 gtgccgctgc cgcaaaaagg atcgagcacg ctgtcgcctt cgttcgacga agcgcggatg
    13681 atgcgttcga gcaacgcttc gggcttctgc gtggggtgct tgccgtaacg cttctcgcgc
    13741 gtcgtgggcg acttgatctg ccagagcgat tgcatctgct tgccgccgtt cgcctgtttc
    13801 atcaacccgt aattgaacgt gtgcttcgcg tgcgggtcgc gcttcgccca gaggatcgtc
    13861 tcggtggcgt gtgtgaaata acgacaggcg agattcggcg gcgggttcac tttgtaccac
    13921 gcgatgtcgt tcaggatttt atagccgagc gtttgcagcg cgaagccgac gctataaatg
    13981 ttgtgcgccg tgcccgtcac ccagatcgcg ccgtgaggcc gcaggagacg tttgcactcg
    14041 ccgagccacg ccacgttgaa agcctgaatg tcatccaccg cgggagcttt gtcccacgtc
    14101 cccttgttca cgctcgcacg cttcccggac tggcacgtca ccccgttgtt cgacaaaaaa
    14161 tagggcgggt cggcaaagat cagatcaaac gactcgctcg ctgccgcgcg caacaattcg
    14221 agcgcgtcgc ctcgatgcag actgagcgcg tgccgccggt tctgaaaaaa cggcttccgc
    14281 gacgacttat gcgccgggcg cgaggttttc gcttcgcgtg tcaatctctt gtttggcagc
    14341 acttggtttg agtgacgagc ctgtctgaaa cgaacgcggc ggattatact ccagatgtcg
    14401 ccgatgcttg accattccgg tagaggttgg gcaagattac gcttgaagcc gtgccgcaaa
    14461 ggtagttttc tccccgcaaa aaagagagtc aaagagagcc gaggttagtc tctaaatgtt
    14521 taagtcgatc ctgattcgtg cgttgtgcgt gagcttgttg tgtgtgtgcg gagcggcggc
    14581 ggcggtgtgc cgtgcgcaga gcttggagcg tgaacttccg gcgggcgcgg gcgtgtcggt
    14641 gtcggttaag aacttgagcg ggcgcgtgac gattgtggcg gcggcggagg gcgaggagcg
    14701 gaagagcgtt tcgttgaagg cggagtcgcc gggcgcggcg gtgcaggagg cggacgtggt
    14761 ttcgaagggg gcgggcgggc agttggaggt cgaggtgcgc gagggacgcg ccgagcgcga
    14821 ccgcatcgac ctgactttgc gcgtgccgca acgcgcgcgt gtgaaggtgg agacgcgcgc
    14881 cggggcggtg gacgtgtcgg gacccgtgag cgaagcggaa gcggcgacgg atacggggac
    14941 gattcgcgca gacgtgccgc tcgattcttt acgctacagt tttcgttgga cggcgagccg
    15001 cccgcgtttt tatagcgaag tggagttggg gaaagttgag gagaagcgcg gcgggcgttt
    15061 cgaggtgtcg ggtcgcttcg gcgacaagaa ggcgaagcgc gagcagcgcg tcaagctgga
    15121 ttttgagacg gagcgcggcg tgattctctt cggcgtcgat ccggcgagcg tgcccgccga
    15181 tttgcgcgag cggcaattga cggaggcggc gcgcgccatc atccgcagcg gcaatcagga
    15241 tttgatcgaa gccatccgca aggtgacgcc gcgcttcgtc ggcgagtacg ccaagacgct
    15301 gcctccgccg cgcgggggcg cacccgtgct aagcgtcgtg gcgaagggcg agcgagccgc
    15361 cgcgcgcgag gtcgcgccgc agttgatgcg cgtcaacgcg aacgtgacgg acaggctggg
    15421 gcgtgccatt gccgggcttg agcgcaaaga cttcagcgtg ctggaaggca gcgagccgcg
    15481 cgaggtggta tcggtcgagc cgacgcgcac gccgttcaat ctggtgttgc tgttggacgt
    15541 gtcggggagc gtcgaggagc gtctggactt catcagaaaa tccgcgctcg ccttcgccaa
    15601 cacggtcagc ccgcaagacc ggatcgccat catcagcttc cgcgacgaca tccaactcat
    15661 ctccgacttc accaccgacc gcaggctctt agcccaacgc atcaaagata tcgaagcggg
    15721 cggcggcacc gcgctctacg acgcgctcgc ctacacgctc gtcaacacct tgaagccgct
    15781 gcgcgacgaa cgcacggcca tcgtcgttat ctccgacggc gacgacaatc gatccttcat
    15841 cccgttccct tacatccttg agacgatcat cgaaaccggc gcggtcgtct acccgctcta
    15901 cattccttcc ggcctcattc ccgcttcggg cgcgcccgct cccttaacga cgctcgaccc
    15961 gacccgcacg cgcttcctct cgctcacgtc gcgcgccgac gaggaagggc gcaaacttgc
    16021 gagcgtctcc ggcggcgtct attaccccgt ccgacgcttc gacgaactcc aactcgccta
    16081 cgacgacatc gtcgcccaac tccgcacctc ttacaccgtc agctatgcca cgacttctgc
    16141 cgccaccgcc acgcgcaacc gcgtccgcgt ccgcgtcgca cgcgaaggcg cggtcgtgcg
    16201 cctgagtccg gtggtcggct cagggacttc gcagccaaac tgagcggctt cattaagcgg
    16261 cttcgcggcc aaactaattc gtacattttg gagacgggcg gcggcgggtg ttaccatccg
    16321 cattgatgaa aagttcgctg ttgttatccc tgttcgacga gacggagcgg cgtccgctct
    16381 ccgtgtcgga gttgacctcg caggtgcgcg gggcgttgga gaaccgtttc gcctcggtgt
    16441 gggtcgaggg cgaaatctca aacttcaagg cggcgagttc cggccactgg tacttcacga
    16501 tcaaggacga gagcgcgcaa cttcatgcga aatgttttcg cggcgtgaac gggcggatac
    16561 gctttcgccc ttcggacggg ttgcaggtgc gtgcgcgcgg aagaatcacc gtctacgagc
    16621 cgcgcggcga gtatgagttg ctcgtcgagg gactcgaccc ggtgggcgcg ggcgcgctgc
    16681 gcgtggcgtt cgagcagatg cgcgaccggc tcgcggcgga aggcttgttt gcagaggagt
    16741 tgaagcggcc tctcccgctt ctgccgcgac gcgtgggcgt cgtcacgtcg ccgacgggcg
    16801 cggccatccg cgacatcatc aacgtcatca agcggcgcac gcgcaccgtc aacatcctcg
    16861 tcgcgccgac gcgcgtgcag ggggtgggcg cgggcgcgga gatcgcgcgt gcgatccgtt
    16921 ctttgaacga acatcatcgc agggcgcagc gcgaggggcg cgtgcgggat tacatagacg
    16981 ccatcatcgt ggggcgcggg ggcggttcga gcgaagacct gtgggctttc aacgaggagg
    17041 aggtggcgcg tgcgattcgc gcgtcggcga tcccggtgat ctctgcggtg gggcatgaga
    17101 cggacttcac catcgcggat ttcgtggcgg acgtgcgtgc gccgacgcct tcggcggctg
    17161 ccgagatcgt ggccgcgcgc gaagatgaga tgactgctat cgtggcgacc ctgacgcgcg
    17221 acctcgtgcg tgcggtcgag tatcgcctgc tcatcgaacg cgagcgcgta cacgaggcgg
    17281 cgagttccga gagtttcgac gaggtgcggg aacggctgcg agcggcgggt cgtatgactg
    17341 atgaactcgc gcggcgtttg gagagttcga ccctgcgtgc gcatgaacgc gcgcggaaga
    17401 ggttggagtc ggtcgtgcaa cggctgtcgc ccgcgcgtgc ggcggcgcgt acgagcgagg
    17461 ggaacgtgcg gctggcgttg gcgcgtgcgg cgctggatgc gaatttcggg gcgcgtctgg
    17521 acgagcgcag ggcgcggctc gaagtggccg tcgcgtcgct cgatgcgctg tcgccgctcg
    17581 gcgtgctggc gcgcggttac gctctgacgc aggacgcgcg cgggcgtgtg ctgcgttcgt
    17641 cgcggagcgt gcgcgcgggc gagcgtgtgc gcgtgcggct ggccgaagga tcgttacaat
    17701 gccgcgtgga agaagtggag ggcgagagct gatgtccacc gtgccgggag agcgtgccaa
    17761 aaaagggaag cgcagccgca tcgtcgtcaa cgtcgagccg aagccgccgg aggagagagg
    17821 cggggcggtg ggacgctggc gcgggaagtc tggcgcgcgg cgcacgggcg ggcgcaggcg
    17881 gcgtgcgttg ctggtggcgg cgattccgtt tgtcgtcatc gcgctcctgt tcttcggcgt
    17941 cggctactgg tggtggcaga gtttcaagag cagccccgcc tacacgctcg cgctcgtggt
    18001 tgacgcggcg gagcgcgacg accgtgaaac gttcgagcgt ctggttgacg tggagggcgt
    18061 gagccggagc gtggtgccgc aggtgatcga gcgcgtgcgc ggcggagaca acgccttaaa
    18121 catcgcgccg caggtgcgcc gccagatcgc gcagaacgcg caggtgtggc tgccgggcgt
    18181 gcgccagcag gtgggcgacg tgttgatggc cgagacgaag gggctggccg agcgcggcgg
    18241 cgcacgcaac tatcccttct tcgtgcgcgc cttcgcgctc tcgcgtctgg gcgagagcca
    18301 gacgcaggag gggacggcgg gacgcgtgcc cacggcgagc gtaacgtatc aagtcaacgg
    18361 acgcccggtt gaattcggat tgctcggcgg cgacggcagc gaggggcaac cggattggaa
    18421 gatcgtctct ttgaagagcg acgatctggc gacgcgcgtg gcggagcagg tggcgaaaat
    18481 tttcccgatg acgggcaggt gagagaaagc gtgaggatga aaaaagcaga agcgcagagc
    18541 cagaatttcg agtcgtcgct ggcggcttta gagaagatcg tgcgcgagtt ggagcgcggc
    18601 gagttgcctc tggaaaagag cctcgaactc ttcgagcgcg gcgtgagact ctcgcgcgaa
    18661 tgccaggagc gtttgcagga agcggagcgg cgcatcgaag tgctcttgca gcgcgacggc
    18721 gaagggcgca gcagcgtgct cggcgcgctc ggcgacgaag agtttgatcg tctcgcgggt
    18781 gtcgaagcgg aagcggacga cgatgaaacc gccttctgac gcgttaatcg aaacggctgg
    18841 cgcgcacgaa accgcgagcg cggaagaggc gcacgcttat ctcgccgccg tgcgcacgct
    18901 cgtcgatgcg agtctcgaac tgctcgtgcc gcccggcaca catgagccgc gagcggtgca
    18961 tgcggcgatg cgttggagtc tcttcgcggg cggcaagcgt ctgcgccccg cgctcgcgct
    19021 ggcctcgggc gaggctttcg gcgcgccccc tctgcgtctc acacgcacgg cctgcgcgtt
    19081 cgaactcata cacacttact cgctcatcca cgacgacctg cccgcgatgg acgacgacga
    19141 tctccggcgc gggcgtccga cctgtcacat caggttcggc gaggcgacgg cgatcctcgc
    19201 gggcgacgcc atgcagacgc tcgcctttca gaccatcgcc gaagactccg agttggatgc
    19261 acacgtgcgc gtgcggctcg tcgcggaact ggcacgcgcg gcgggcacgc ctgccgggat
    19321 ggtcgcgggg caggcgcatg atctggcggg ggagtcgcgc acggatgtca ccgccgacga
    19381 actcgattcc atccaccgcc acaagacggg cgcgctcatc gtcgcggcgg cgcgcgcggg
    19441 cgcgattgtc gcccaagcga gcgaacgcga gatcgaacgc atcagcgact atgccgccgc
    19501 gctcggcctt ctgtttcaga tcacagacga cctgctcgac gtgacggcga ccgccgaaac
    19561 tttaggcaag acgcccggca aagacgcacg cgcgcagaag gccacctacc ccgcgctcca
    19621 cggcctggag gcgacgcagg aacgcgccct cgccgtctac acgcaggctc tcgacgccct
    19681 cgctcagatc gaaagaccga cccgcctgct gcgctccatc gcgcgtctca tcctcgaacg
    19741 ccgcacctga ttttcttgac agcccttcgc gcccgtttgc ataattgcgc cagagccgga
    19801 caaaacggat catctcacct cctcaaagat gatcgcctac gcaaaacttc cgctgtcgcg
    19861 tgcggcagtt ctctcgtcga acccaacttc gaccagactc atccgccggg gagtctctta
    19921 tgagttacac caaaagattg atcttgagcc tgcttgtcgc tgtctccgcg tcgcttgccc
    19981 tgagcgtgtg ccttgcccgc gcgtactcga acgaaacgag gccggagaca caggatgcca
    20041 cgcttgccgt cgtcgccgcc gcgccggaaa agttcggcgc gattccctcc accacgccca
    20101 tcatcgggca gacgcggctc gtcttcacga gcgaccgcga cggcaacgcg gaggtctatt
    20161 cccaggcggg cgacaacagc ccgcaggtca atctcacgaa caacccggcg acggacgcct
    20221 gccccgtcat ctctcccgac ggcacgaaga tcgccttcac gagtaatcgt aacggtcaat
    20281 cggacatcta cgtgatgaac acggacggtt caaacgttca ggccgtcacg catttcggcg
    20341 acgggggcgg cgttaacacg cgcgcctacg atccggcgtg gtcgccggac ggcacgaagc
    20401 tggcttacgt tgccagcttt tttggcgggg aatcctccgg gcttttcgtg gtgaacgcgg
    20461 acggcagcgg cgaagctgcc cacattggca acggctcgga agcagccgac cccgcgtggt
    20521 cgcccgacgg cacgcgcatc gcctatgtcg ggcgcgaagg aagttacttc ccgacggaag
    20581 agggctttcc ctacttcctc tttgtgacga acgcggacgg cagcggcacc acgcgcatcg
    20641 ccaccgatac gcccatcttc tcgtcgttcg cctacccgcc cgaagcgagt ggtcccagtt
    20701 ggtcgcgcga cggggcgcgt ctcgccttcg tcagcaaccg ccacggcaac gaggagattt
    20761 acaccgtcag cgcgacgggc ggcgcggtca tgcgcctgac gaacaacccc gcgacggaca
    20821 ctttgccgac gtggctcggc gacggcatcg cgttcaccag cacacgcggc ggcgcgcgcg
    20881 acgtttacgt gatgtatccg ggcagtcctt tgatctcggg cgagcctttg atgtttcgca
    20941 tcaccggcaa cgcgggcaac aatttcgacg ccgactggcg ttcacttacg cccgccgcgc
    21001 cgtcaacgat ctccgcctcg cgcatcgcct tcatccgcgc caacccggcc aaccctgatg
    21061 acacggacat ctacacgtcg aaccccgacg gcacgaatga agtgaacatc accaacagcc
    21121 cgcagaagga gtttgcgccc gcgtggtcgc ccgacggcaa gatgatcgcc tacgtgcgtc
    21181 gcccggagcg cgcgctcttt gtgatgaatg ccgacggcac gaacgcgcgc gaactcaccc
    21241 ccgtcagcga cgttgccgtc aagccttcgt ggtcgcccga tggccgccgc atcgccttca
    21301 tcggcggcag cgatctggcc tacacgctcg ccgtcgtcaa cgtggacggc acggagcgca
    21361 ggcgactcgt cccgctcgac gccacttatc acgaaatcgc ctggtcgccc gacggcacgc
    21421 gccttgccta cgtgttagac cgcgcctccg cctttgccga tccggccatt cacgtcgtca
    21481 atctcgacgg cacgggcgca cgcatgctct cgaccggcat caatcgcgac cgtcagcctg
    21541 cgtggtcgcc cgacagttct aaactcgctt tcacctcgac acgcgacgcc aacgtcgaaa
    21601 tctactcgat gaatgccgac gggacgaatc aaacgcggct cacgaataat ccggcgacgg
    21661 acgcctcgcc tatctggtcg cccgacggcc agacaataat cttctccacc aaccgcgacg
    21721 gcgacttcga gctttaccgc atgaacgctg ccgacggcgg cggacaggcg cgcatcaccg
    21781 acacaagcgc aaatgaggtc gcgcccgact ggaatcgggg cgctctctcc accatccagt
    21841 ggcgccaccc ttcgttccgc atccgcgaag gcttggagca cggcagcagc ggcgaaccgc
    21901 tcgtcgtcac gcgcctcggc gacctgacgc accccgcaac cgtcgaatac gcgacgacgc
    21961 acggttgccg cgacgcgttc gacgccccgc cgtccatgtg cggcagcgga ttcgcttccg
    22021 aacgttccga ttacgtgagc acgacgggca cgctccgctt cgcggcgggc gaagccagca
    22081 agaccatcag cgtgaccacg attgacgacg ccatcatcga aggcgacgag cgtttcaatc
    22141 tcgtcctgac gaatgcgacg ggcgcgacgc tcggcgagca gcgcgacgtg ccggttatca
    22201 tcaccgacaa cgacaaaaac gccgccgcgc ccaaccccat tgacgagccg cgcttcttcg
    22261 tccgcatgca ctatctcgat tttctggggc gcgaaccgga gcagagaggc tgggatgatt
    22321 gggtcggcgt ctggacgcgc tgcccgaaca tcttcaacaa tccggtgtgc gaccgcgtga
    22381 cgatctccgc ctccttcttc cgctcgcgcg agttctacgt gaagagctat tacatctatc
    22441 tcttctatcg cgccgcgctg gggcgacgac ccacctacgc agagtttgtg cgcgacctcc
    22501 cgcgtctcgc ggcgcagacc ggcgcggagt tggacgcgcg caaagatgat tacaacctcg
    22561 cgtggataca ccgcgcggag ttcgcagagg tcgcccgcac gttcacgcat gagcagtttg
    22621 ttgaccgtct cgtgcagaac gtcggcatca cgctgacggg cgcggtgacg cgcgagacgc
    22681 tgctcgcgga tttgcgcgcc ggacgcagga cgcgcgccgc catcctgcgc gccgtcgtcg
    22741 agcacccgga cgtggagcgc gcggagtaca acggcgcgtt tgtcacgatg cagtatttcg
    22801 gctatctgaa gcgcgacccg gacgccacgg ggttcgccaa ctggctgaac tacctgaacg
    22861 cgcacccgac ggactttcgc acgatggtgc acggcttcgt cgtctctaac gaataccgcg
    22921 cgcgcttcgg aaaaccttga gacaggataa gagcaagggg aaagagtcag gggtcaaggc
    22981 aaagggcgga aagccttcaa agaagcgggg cgcagcattt gagatgctgc gccccgcttc
    23041 ctgtctttac ctttaaactt aaccttaaac ccctgaccct ttagccttaa atttccccct
    23101 gacctttttc cttaaagttt cagactccgg ttgtcggctg gtgcgtcgcc acctttcgct
    23161 gcaacgcgcg cagagtttcg ggcatgaagg ccgtgagatg atgcgtggcg gtgtggccgt
    23221 cggcgaacgc ctgatagagc atgcagtcgg ggagttgcag gcaactgagg ggcgacgagt
    23281 tgatgtagcc gctgtcgatg ccgatggcgc tgtgcgagat gtagatgccg tgacgaccga
    23341 ggcgtccgcg caggggcaac agcggcgcgg gcgtgtggcc gaagacgcag ggcttgcccg
    23401 tgtagttgcg aaagaaatcg ctgtcgcgcg accagaggag atgctgcggc ggcgtgtcgc
    23461 gcgggtgctg tcccttgctc aacccggcgt ggacgtagag cgcgtagtcg tcttcgtgaa
    23521 agagcgggag ggtgcggaag aagtcgaggt gcgcgggcgg aagcgactct gtgaaatctt
    23581 ggcgcagcgt cgtgaaatcc tgttcgctgc gcacgtgcag aggcttgcct gtgtactgtc
    23641 tgaaagtgca ttcgctgccg accgccgcgt gcagccagag cgtcgcgctc tcgtccacga
    23701 agtcgagcaa catctgttcg tgattgccgc gcaggcagag cacgcgctcg ggctgttcgt
    23761 ctttgagctt caccacgtcc gccaccgtgc cgggcacatc atcgccgcga tcaatgatgt
    23821 cgcccagcag gatgagcatg tcgtgcgaag cgtcgcgcgg aatgatctca aggagatgat
    23881 tcagttgcgc gcgtcgcccg tgcacatcgc cgatgacgaa ggttttcatt taagttcaac
    23941 ccgttcttcg cacgtcaaac tccggcctgc cgtccgctgc cgttcgagcc gcttattcta
    24001 ccccgactct cgcctgcgcg tgcgatttct cgatgcgctc catcaacgtc tcaaagcgcg
    24061 ggtcggctct gagttccgtc cattcgggat tcgcacggaa ccagggcaga ttttcgttgc
    24121 cgcgattgat ggcgcgctcc aaccatttga acgcctgatc gcgcatgcct tccacggcat
    24181 aggccgtcgc gatccagtaa ggcgcgtcgt ggtctgcttc ggccacttcc ttgacgcgct
    24241 cggtcaactg cgcgcgtgcc gcttcgtgct ggccgagctt gctgagcgcg atggcatagt
    24301 ggggacggat gccgtccatc tccgggtgcg tgtcgagcac gtcgcgcaaa atttccgccg
    24361 cccccgccgc gttgccctga cgatagaagg cgaccgcgcg gaaggtcttg acgaacggat
    24421 gatcgggttc gatccgcgcg ccgtcgtcga gtgcgagcat ggcctcgtcc atgcgcccct
    24481 gatacatcag gacacgcgcg cggttgtagc tgaccacgac gcgctccgca gggttgagcg
    24541 cgagcatgcg gtcgagactg cgcagggagc gttcgtaatc gccgtcgagc cggtagagat
    24601 aagaggcgac aaactgcacg ccgacatcgt tcggggcttc gtgccgcagg ttctccaccg
    24661 ccgcgcgcgc tttcgatttc tcgccgcgcg ccagatagat gaagaccatg tgcatgcgcg
    24721 cttccatgat cttcggatca atgtccagac ctttgcggaa tgcctcttcg gcgcgctcgt
    24781 agtcctcgaa atcgcccatc gacttgatga cgcgattggc gtagcagccg ccgagcgcgc
    24841 aatgcgcgag cgcgaaattg gggtcgagtt cgatggcgcg tttgaaatgt tcgatggcct
    24901 cttccacatc ttcgttggcg agcgtgtgat agatgtaacg ccccatcgca tcgcgcccgc
    24961 gcaggtagat gttgtaagcc tccgggttgg ctgtcgagcg ttgggcgagg ccgctctttt
    25021 cgtccgcgct cagttcgacg cgcaagcctt cgacgatgcg ctgcgcgatg gtgtcctgca
    25081 cggcgatgat gtcggcggcg gtcgcgtcga tgcggtctgt ccagagcagg tcgccggaag
    25141 ccacgtcgag cagttgcgcg ttgactctga gcttatcgcc cgcgcgcagg aaactcgcgg
    25201 ccagcaccgc gccgacgctc aactcctcgc cgatctcgcg cgggtcgcgc tcctgtccct
    25261 gatacttcac gatcagggac gacgggcgca cgacgagcga gcgcaaacgt gcgagttccg
    25321 tgatcactgc gtcggcgagc gaaaattcgt agaagctcac ttccgcgtcg ccgctcaaat
    25381 tgcgaaaggg caggatcgcg atagattttt gcgcgctctc ggaaaggttc gtcccgtgct
    25441 gcggttcgcc ttcggagcgg tgcggcgcag ccgagatgtt gcgcgaagcg gaggcgttag
    25501 gcggcgcgga agttgactgc gcgctcgtcg gcgtctccgt gcccgtgatg ttgcgcagcc
    25561 agcgacgaaa ggtgcgaccg aaagaggaat tgctcgattg atggcgcggc gcaaccggct
    25621 caaactcgtc ggtcgtatcg atggagtttc ctccgcctgc ggctgcgccc actgccgcga
    25681 gttcgcgttt gacggcgcgc aggtcgtcgc gaaactccgc cacgcgttga taacgatcct
    25741 tcgggtcttt ggcgagcgcg cggtcgagta tctcctgcaa gcgcggcggc gcgtcgcgcc
    25801 tgaattcgcc tacgcggacg ggcgtgtcgt gcaggacggc gtagcgcacc tcaacgttcg
    25861 tcttgccctg aaacggccag atgcccgcca gcatctcgta gagcaggacg ccggtggaga
    25921 agatgtctgc gcgatgatcg acgtgcgcgc ccgtggcctg ttcgggcgcg gcgtaggtgg
    25981 ccgtgccgta agggacgccc atctcggtca gatgttgctc catgcccttg ccgccgctct
    26041 tgttttcgtc gagcagttta gccagaccga aatcgagcac tttggccgtg ccgttcttcg
    26101 tcaccatcac gttgcccgct ttgatgtcgc gatggataac gccgcgcgcg tgtgcggcgg
    26161 ccagcgcgtc tgcgacctgc atggtgatgg agatcgcgct gcgaagttcg agcgggcg
//
LOCUS       DASWNS010000160         6595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_32898, whole genome shotgun sequence.
ACCESSION   DASWNS010000160 DASWNS010000000
VERSION     DASWNS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32898"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="VGB76_05540"
     CDS             <1..163
                     /locus_tag="VGB76_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY0098399.1"
                     /translation="IVGAARSRGEKTMIDRLTGELLDAASNRGNAVKKKDDVHRMAEA
                     NKAFAHYKW"
     gene            255..2351
                     /gene="fusA"
                     /locus_tag="VGB76_05545"
     CDS             255..2351
                     /gene="fusA"
                     /locus_tag="VGB76_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0098400.1"
                     /translation="MAKQDLRKFRNIGIMAHIDAGKTTTTERVLFYTGVSHKIGEVHE
                     GTATMDWMEQEQERGITITSAATTCFWKRNNVEHRINIIDTPGHVDFTMEVERSLRVL
                     DGAVAVFDGVAGVEPQSETVWRQADKYGVPRICFINKLDRAGASFQRSFDSIVTRLGA
                     NPVAIQIPIGTEDQFKGVIDLIKMKALLWNDETKGAEYAIEEIPADMREEADAAREKM
                     IEAIASIDDDLTNKFLEGEEISEDELRAALRKGTIEIKIVPVVTGSAFKNKGVQTLLD
                     AVVDFLPSPLDIEAIKGTNPKTGEEEPRPADESAPFAGLVFKIMADKHLGQLSFVRIY
                     SGTIKGGSYAFNSVKGTKERVGRLLRMHANKREDIDEASAGEIIAIGGMKQVTTGDTV
                     CDENKPIILEAMDFPAPVIRVAVEPKTRADQDKLGTALSRLAQEDPSFNVSTDQETGQ
                     TIIAGMGELHLEIIVDRMKREFGVEANVGKPQVAYRETITQPAPGKEVYKRQTGGRGQ
                     FGHVEIEIEPAPGEGFVFENKITGGVIPREYIKPTEEGIRDAMERGFLAGYELVDIRV
                     RLVFGSYHEVDSDERSFHIAGSLAFQDAVRKAKPVLLEPIMRVEVVTPEEYMGSVNGD
                     LNRRRGQIEKMEPRTGGVQVITAFVPLSEMFGYTTDLRSSTQGRATSTMHFERYEQAP
                     KNIAEEVIAKVQGVTK"
     gene            2461..3648
                     /gene="tuf"
                     /locus_tag="VGB76_05550"
     CDS             2461..3648
                     /gene="tuf"
                     /locus_tag="VGB76_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY0098401.1"
                     /translation="MSKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVLAKHNPKIT
                     VRAFDSIDNAPEEKARGITIATAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVGVPAMVVFMNKVDAVDDAELLELVELEVRELLS
                     AYEFPGDDIPVIQGSALGALNGEAQWEAKLDELMAAVDSYIPTPERDVEKPFLMPVED
                     IFTIQGRGTVATGRVERGRVKVNETVEIIGIRDTRTTVCTGVEMFKKLLDEGVAGDNV
                     GLLLRGVERKDIERGQVIAKPGSITPHTKFKAEAYILTKEEGGRHTPFFTGYRPQFYF
                     RTTDVTGVASLPEGVEMVMPGDNVAMEIELITPIAMEKGLRFAIREGGRTVGAGTISE
                     IVE"
     gene            3707..3859
                     /gene="rpmG"
                     /locus_tag="VGB76_05555"
     CDS             3707..3859
                     /gene="rpmG"
                     /locus_tag="VGB76_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY0098402.1"
                     /translation="MPRDNVVLQCTECKHRNYVTTKNKKTTTERLEFSKFCRFCRCHR
                     NHKETK"
     gene            3950..4025
                     /locus_tag="VGB76_05560"
     tRNA            3950..4025
                     /locus_tag="VGB76_05560"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3984..3986,aa:Trp,seq:cca)
     gene            4053..4454
                     /gene="secE"
                     /locus_tag="VGB76_05565"
     CDS             4053..4454
                     /gene="secE"
                     /locus_tag="VGB76_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY0098403.1"
                     /translation="MARQKDDEFENEDDKSLPSLEPTPPPGRTGSSDVEAGTGPAPRA
                     ARFGEGPEGRAARRERGVQGAREGFFGRIVTFAHDIRAEMRRVSWPGLKDVQNTTIIT
                     IIAVAFFATYLWLIDKGWTFLINGLTKALGG"
     gene            4535..5095
                     /gene="nusG"
                     /locus_tag="VGB76_05570"
     CDS             4535..5095
                     /gene="nusG"
                     /locus_tag="VGB76_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY0098404.1"
                     /translation="MQQWYILHTYSGHEKKVRDSLKARIRDTDLQAEITEVLVPTETV
                     VEMRGNKRVESSRMFYPGYVLVQIECDERGHIPDNVFHLIKSTPKVTGFVGGQNPTPL
                     TPQEVDQIVHNVTEAQEKPKPKFTYAAGETVRIKSGPFASFTGKVEEVNADKSTLKVV
                     VTIFGRSTPVELNFLDVEKVTFTEDE"
     gene            5181..5606
                     /gene="rplK"
                     /locus_tag="VGB76_05575"
     CDS             5181..5606
                     /gene="rplK"
                     /locus_tag="VGB76_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY0098405.1"
                     /translation="MAKKITGYIKLQVPAGKANPAPPIGPALGQHGVNIMEFCKAFNA
                     KTAQMDPDMKIPVVITVFADRSFSFETKTPPAADLLKKAAGIPKGSGKPNKEKVGTIS
                     RAKIEEIARAKMPDLNTTSLESAIKTIEGTAVNMGLTVE"
     gene            5732..6427
                     /gene="rplA"
                     /locus_tag="VGB76_05580"
     CDS             5732..6427
                     /gene="rplA"
                     /locus_tag="VGB76_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY0098406.1"
                     /translation="MAKHGKKFRAALEKIEPGRKYNLDEGLAKVKEISFAKFDETIEL
                     TMWLGVDPRKADQLVRGTVVLPHGLGKSKRVLVIAQGDKIREAEEAGADFVGGAEMVD
                     RIKGGWLDFDAVIATPDMMRLVGGLGKVLGPRGLMPNPKTGTVTPDVTTAVRETKAGK
                     VEYRVDKTGVIHVGIGKVSFDQQLLSENAQTLLNAVVRAKPSTAKGKYVKKVNLASTM
                     SPGVLLDETAYVK"
BASE COUNT         1579 a         1816 c         2025 g         1175 t
ORIGIN      
        1 gatcgtgggc gcggctcgct cgcgcggcga aaagacgatg attgaccggc tcacgggcga
       61 actgctcgac gccgccagca atcgcggcaa cgccgtcaag aaaaaggatg acgtgcatcg
      121 catggcggag gccaacaaag cctttgcgca ctataagtgg taagagacaa gtaatgagta
      181 atgagtaatg agtgatgagc ggaagacgct tctaaactca ttactcatcg cccatcactc
      241 atcactataa gatcatggct aagcaagacc tcaggaaatt ccgcaacatc ggcatcatgg
      301 cgcacattga tgccggaaag acgaccacca ccgaacgcgt gttgttctac acgggcgtct
      361 cgcacaagat cggcgaggtg cacgaaggca ctgcgacgat ggactggatg gagcaggagc
      421 aggagcgcgg catcacgatc acgtctgccg ccaccacctg tttctggaag cgcaataatg
      481 tcgagcatcg catcaacatt attgacactc ccggccacgt cgatttcacg atggaagttg
      541 aacgcagctt acgcgtcctc gacggcgcgg tcgccgtgtt cgacggcgtg gcgggcgtcg
      601 agccgcagtc ggagacggtc tggcgtcagg ccgacaagta cggcgtgccg cgcatctgct
      661 tcatcaacaa actcgaccgt gcgggcgcat ccttccaacg ctccttcgat tccattgtta
      721 cgcgtctcgg cgcgaacccc gtcgccatcc agattccgat cggcacggaa gaccagttca
      781 aaggcgtcat cgatttgatc aagatgaagg cgcttttgtg gaacgacgag acgaagggcg
      841 cggaatacgc gattgaagag attcccgccg acatgcgtga ggaagcggac gccgcgcgcg
      901 aaaagatgat cgaagccatc gccagcatcg acgatgattt gacgaacaag tttttggaag
      961 gcgaagagat tagcgaagac gaactccgcg ccgctctgcg caaaggcacg attgagatca
     1021 agatcgttcc cgtcgtgacc ggctcggcct tcaagaacaa gggcgtgcag acgttgctcg
     1081 acgcggtcgt cgattttctg ccttcgccgc tcgacatcga agcgattaag ggcacgaatc
     1141 ccaagaccgg cgaggaagag ccgcgcccgg cagacgagag tgcgccgttc gccggtctgg
     1201 tcttcaagat catggccgac aagcatctcg gccaactctc tttcgtacgc atctattcgg
     1261 gcacgatcaa gggcggctct tatgctttca actcggtcaa aggcacgaaa gagcgcgtcg
     1321 gacgcctgct gcgcatgcac gccaacaagc gcgaagacat cgacgaggca tcggctggcg
     1381 agatcatcgc catcggcggc atgaagcagg tgacgaccgg cgacaccgtc tgcgacgaga
     1441 acaagccgat catcctcgaa gcgatggact tcccggctcc cgtcatccgc gtcgccgtcg
     1501 aaccgaagac gcgcgccgat caggacaaac tcggaaccgc cctgtcgcgt ctcgcgcagg
     1561 aagaccccag tttcaacgtc tctacggatc aggaaaccgg ccagaccatc attgccggaa
     1621 tgggcgaact ccacctggag atcatcgtgg atcgcatgaa gcgcgagttc ggcgtcgagg
     1681 ccaacgtcgg caagccgcag gtcgcctatc gcgagacgat cacgcagccc gcacccggca
     1741 aggaagtcta taaacggcag acgggtggac gcggccagtt cggccacgtc gagatcgaga
     1801 tcgaacccgc accgggcgaa ggcttcgtct tcgagaacaa gatcacgggc ggcgtcatcc
     1861 cgcgcgagta catcaagccg acggaagaag gcatccgcga tgcgatggag cgcggcttcc
     1921 tcgccggtta tgaactggtg gacatccgcg tgcgcctcgt cttcggttcg tatcacgaag
     1981 tcgattccga cgagcgttcg ttccacatcg caggctcgct cgcgtttcag gacgcggtgc
     2041 gcaaggccaa gcccgtgctg ctcgaaccga tcatgcgcgt cgaagtggtg acgccggaag
     2101 agtacatggg atcggtcaac ggcgacttga atcgtcgtcg cggccagatc gaaaagatgg
     2161 agccgcgcac gggcggcgtg caggtcatca cggccttcgt cccgctctcg gagatgttcg
     2221 gctacacgac cgacctgcgc tcgtccacgc agggacgcgc cacttcgacg atgcactttg
     2281 agcgttacga acaagcgccg aagaatatcg cagaggaagt gatcgcgaag gtgcagggcg
     2341 ttacgaaata agtttgagcc agacgaatta agaaagcggg tcgcttctct cgctgcacga
     2401 caggcgcggc gacccaatag acagaaaccg ataaataatt tgtaggagcg agagaaagac
     2461 atgagcaagg agaaattcga ccggtcgaag ccgcacgtca acatcggcac catcgggcac
     2521 gtcgatcacg ggaagaccac cttaacggct gcgattacga aagtcttagc caaacataac
     2581 cccaagatca ccgtccgcgc gtttgattcc atcgacaacg cgcccgaaga gaaggccaga
     2641 ggcatcacca ttgccaccgc ccacgtcgaa tacgagacgg ccaaccgcca ctacgcacac
     2701 gtcgattgtc ccgggcatgc cgattatgtc aagaacatga tcaccggcgc agcccagatg
     2761 gatggcgcta ttctcgtcgt cgccgccacc gacggtccca tgccgcagac ccgcgagcat
     2821 atcctgttag cccgtcaggt gggcgtgccc gccatggtcg tctttatgaa caaggtcgat
     2881 gccgtcgatg acgccgaact gctcgaactc gtcgaactcg aagtcagaga actcctctcg
     2941 gcttacgagt ttcccggcga cgacattccc gtcattcaag gctcggctct gggcgcactc
     3001 aacggcgaag cgcaatggga agccaaactc gacgagttga tggcggctgt cgatagctac
     3061 atcccgacgc cggagcgcga cgtggagaag ccgttcctga tgccggtgga agatatcttc
     3121 accattcagg gacgcgggac ggtggcgaca gggcgtgtgg agcgcgggcg agtgaaggtc
     3181 aacgagacgg tggagatcat cggcatccgc gacacgagaa cgaccgtctg cacgggcgtc
     3241 gagatgttca agaagttgtt ggatgaaggc gtcgcgggag acaacgtcgg gttgctgctg
     3301 cgcggtgtgg agcgcaagga catcgagcgc gggcaggtga ttgccaagcc cggcagcatc
     3361 acgccgcaca cgaagttcaa ggcggaagcc tacattttga cgaaagagga aggcggcagg
     3421 cacacgccgt tctttacggg ataccgaccg cagttctact ttcggacgac ggatgtgacg
     3481 ggtgtcgcgt ctctgccgga gggagtggag atggtgatgc cgggcgataa cgtggcgatg
     3541 gagatcgagc tgattacgcc gattgcgatg gagaagggct tacggttcgc cattcgcgaa
     3601 ggcggaagga cggtcggggc tggtaccatc tccgaaatcg tagaataaga ttttcagggc
     3661 taaagagagc aagttcaaca tagcccgtct gtgaggaagg taagttatgc cgcgcgataa
     3721 tgttgtgttg cagtgcacgg aatgtaagca tcgtaactat gttacgacca agaataagaa
     3781 gacgacgacg gaacgtttgg agttcagcaa gttctgccgt ttctgccgtt gccacagaaa
     3841 ccacaaggaa acgaaatagc agtggcgggt gacaggtgac gggtgacaag ttaaaacaag
     3901 aacttgtctt caactcctca cttgtcattc gtcgctcgtc actctcctta ggggtatagt
     3961 gtcaacggtt agcacgtcgg tctccaaaac cgaaagttcg ggttcaaatc ctgatacccc
     4021 tgccagcacg tttgtgaaga ggtagaactc ccttggcgag acaaaaagac gacgagtttg
     4081 agaacgaaga cgacaagtca ttgccgtctc tggagccgac acctccgccg gggcgcacgg
     4141 gttcgtcgga cgtggaagca ggcacgggtc ccgctccgcg tgcggcacgt ttcggcgaag
     4201 gacccgaggg gcgcgcggcg cggcgtgagc gcggcgtgca gggcgcgcgc gagggattct
     4261 tcgggcgcat cgtaaccttc gcgcatgaca ttcgcgcgga gatgcggcgc gtctcctggc
     4321 cgggtttgaa agacgtgcag aacacgacga ttatcacgat cattgccgtg gctttctttg
     4381 cgacttactt gtggctcatc gataaagggt ggacgtttct gatcaacggg ctgaccaaag
     4441 cgttgggcgg ttgataaatt ttcggacgca gtagttgccc gcttccggtt cgcgtgagaa
     4501 taagctcttt gaaagagcaa ggacgtttta gaagatgcag cagtggtaca tcctacatac
     4561 ctattcggga cacgagaaga aggtgcgcga ttcgctgaag gcgcgcatcc gcgacaccga
     4621 tctccaggcc gagatcacgg aagtgctcgt gccgactgag acggtcgtgg aaatgcgcgg
     4681 caacaagcgt gtcgaatcgt cgcgcatgtt ctatcccggc tacgtgctcg tgcagatcga
     4741 gtgcgacgag cgcggccaca ttcccgacaa cgtgtttcac ctgatcaagt ccacgccgaa
     4801 agtgacgggc ttcgtcggcg ggcaaaaccc gacgcccctg acgccgcagg aagtcgatca
     4861 gatcgtgcat aacgtgacgg aggcgcagga gaagccgaaa ccgaagttca cttacgcggc
     4921 gggcgaaacg gtgcgcatca agagcggccc cttcgccagt tttacgggca aggttgaaga
     4981 ggtcaacgcc gacaagtcaa ctctgaaagt cgtcgtgacc atcttcggac gctcgacccc
     5041 cgttgaattg aattttctgg acgtggaaaa agtaacgttt accgaggacg aataatgaag
     5101 taatgagtga tgagtgatga gtgaggagca actgctctta actcaccatt catcactcat
     5161 cactcatcac tcataaagtt atggcaaaga agattacagg ttatatcaag ttgcaagttc
     5221 cggcggggaa ggccaatccg gctccgccca tcggcccggc actcggccag cacggcgtga
     5281 acatcatgga gttctgcaaa gccttcaacg cgaagaccgc gcagatggac ccggatatga
     5341 aaattccggt cgtcatcaca gtgttcgccg accgctcgtt cagtttcgag acgaagacgc
     5401 cgcccgccgc agaccttttg aagaaagcag ccggcatccc gaagggttcg ggcaagccga
     5461 acaaggagaa ggtcggcacg atctcgcgcg ccaagatcga ggagatcgca cgcgccaaga
     5521 tgcccgacct gaacacgacg agtctggaga gcgcgatcaa gaccatcgaa ggcaccgcgg
     5581 tcaacatggg actcacggtt gaatagagtt tagccatcgg atgccggaaa gccacagagg
     5641 ctgctcgcag gcatctaata tctaaaatct ctcttgagag ggagaaagcg cgccactcgc
     5701 gcgttttcgt ttgcacctcg gaggacgagt tatggcaaag catggcaaga agttccgtgc
     5761 ggctctggaa aagattgagc cggggcggaa atacaatttg gacgaaggac tggcgaaggt
     5821 caaagagatc tccttcgcga agtttgatga aaccatcgaa ttgacgatgt ggctcggcgt
     5881 cgatccgcgc aaagcggatc aactcgtgcg cggcacggtc gttctgccgc acggtctggg
     5941 caagtcgaag cgcgtgctcg tcatcgcgca gggcgataag attcgcgagg ccgaagaggc
     6001 gggcgcggat ttcgtcggcg gcgcggagat ggttgaccgc atcaagggcg gctggctcga
     6061 tttcgatgcc gtcatcgcga cgccggacat gatgcgtctc gtcggcggac tcggcaaagt
     6121 cctcgggcct cgcggcctga tgccgaaccc gaagaccggg acggtgacgc ccgatgtgac
     6181 gaccgccgtg cgcgagacga aggccggtaa ggtcgagtat cgcgtagaca agacgggcgt
     6241 gattcacgtc ggcatcggca aagtctcttt cgatcagcaa ctcttgagcg agaacgcgca
     6301 gacgctgctc aacgcggtcg tccgtgcgaa gccttcgacg gccaaaggca agtatgtcaa
     6361 gaaagtgaat ctcgcatcaa cgatgagtcc cggcgtactg ctggacgaga cggcttacgt
     6421 gaaatagcga gtgaggagcg tgatagagaa atgaaaacca gagaacagaa acagaaagac
     6481 ctcgaagccc tcacgcagca gtttcaggaa gcgaccgccg cgatggtgat gggtgtcaac
     6541 aaattgtcgg tgacccagga tcaggagttg cgccgccagt tggatcaggt gggtg
//
LOCUS       DASWNS010000161         6924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_84646, whole genome shotgun sequence.
ACCESSION   DASWNS010000161 DASWNS010000000
VERSION     DASWNS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84646"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VGB76_05585"
     CDS             <1..294
                     /locus_tag="VGB76_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098407.1"
                     /translation="KPTLTWSQRRTRRILGHHDRAFNTIVISKTLDAPDVPEWFVEYV
                     LYHEMLHIKHPARVVKGRRYSHTKAFLAEEERFPLFDQAQEWLDHVARERRAA"
     gene            653..883
                     /locus_tag="VGB76_05590"
     CDS             653..883
                     /locus_tag="VGB76_05590"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEY0098408.1"
                     /translation="MGESVFKGVEMVGTSDQSFSHAIEVAVKRARQSLRNLSWFTVKE
                     QRGGLQDGRLEYQVTLELFFKLEAMENDPVED"
     gene            1054..1617
                     /gene="efp"
                     /locus_tag="VGB76_05595"
     CDS             1054..1617
                     /gene="efp"
                     /locus_tag="VGB76_05595"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY0098409.1"
                     /translation="MAIPATQIRRGMVILFEGNLCKVIDFRHHTPGNLRAMVQTKMRN
                     LRTGSSFEHRFRSSDTVDKASLEQHEMEYMYSDGSQHHFMNTENYEQTALTEEDLGDM
                     AQWLMPGMKIQAEFYEGAPIGITLPPSMELTVTRTEPTLKGATVSNVNKPATLENGVT
                     ITVPPFVNEGDRIRVDPTEGRYIERAK"
     gene            1702..3036
                     /locus_tag="VGB76_05600"
     CDS             1702..3036
                     /locus_tag="VGB76_05600"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEY0098410.1"
                     /translation="MYLLYSLLLTFAIFALLPLFLLDALRHGKYVAGLRERLGGVPEV
                     DANGRAVVWLHCVSVGETQAARPLARALVERFPSHALVVSTTTLTGQTLAREVFRDEA
                     VAVFYFPFDWAWSVRRSLRRIKPSLVLVMETELWPRFLRECRSAGVPVALVNGRISEK
                     SFRGYRKLGAFIKSVTGALTLAIMQTEADAERIRALGLAPERTRVSGNIKFDADTEVG
                     AAVITRELRERFAFGDERPLIVAASTHAPEEAVTLDAFRQARAAFDRDDSRCPRLLIA
                     PRHPERFNEVAALLASSGLSWARRSVSPKADDATCDVVLLDTIGELRGVYSLAALVFV
                     GGSIAPTGGHNILEPAAAACCIITGAHTFNFASIMRDFLEQDALVQLPTLTEAQAPAV
                     LAQTFRELLNDNERRRRTGDRARAALEHSRGATDKTIALLTPLLEQRPESRG"
     gene            3134..4186
                     /gene="lpxK"
                     /locus_tag="VGB76_05605"
     CDS             3134..4186
                     /gene="lpxK"
                     /locus_tag="VGB76_05605"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY0098411.1"
                     /translation="MSVAYGAAGRARRALYRAGVLPVERVAAPVVSVGNITAGGTGKT
                     PLVEWLARAAASEGRRVCILTRGYGRADASKRVVVSDGEHVMAEAREGGDEPRLLAEN
                     LLEHKVAVVSDANRAAAARWALAHLRSDAFILDDGFQHLRLSRDLNILTLDATDPWGG
                     MKLLPRGRLREPPGELKRADLIVITRAELAVDIEEVRARAEALSGGRRPVLIARTATL
                     RMKLLAVPHSELKVRAHEHEIESVPQTLAAFCAIGNPQAFFAHARRDGLDIIHARAFT
                     DHHVYTQSDIDLYVREAVRQGARALLTTAKDAVKLRALNFALPCYVLEVALKFDDEQK
                     MRAILREALARRLSEL"
     gene            complement(4181..5857)
                     /locus_tag="VGB76_05610"
     CDS             complement(4181..5857)
                     /locus_tag="VGB76_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0098412.1"
                     /translation="MKKCPKCGKTYENAPTLCPADGAVLVKTGDALVGQTLASKYRIE
                     ERISEGGMGTVYRATHVLMEKAVAIKVLHPSLAADDKIVARFTREAKAASRISNPHAL
                     NVTDFGESENGIVFLVMEYLRGKTLKEVIRGEGPMPLARVVEVTRQICGALDAAHAEG
                     VVHRDLKSDNIMLEEVAGGSDWAKVLDFGIAKILETEGPDPELTAPNLIIGTPQYMSP
                     EQCSQASGIDARSDIYSLGIILYEMLVGHVPFTGESATAIMLKHLQEPPPSVLDERKD
                     VPPAVAQVIARALSKMPEDRQASAGELAEALASAAADAPDVQTASVGGETSENAQLGS
                     GNTNRIVVPTAPLESRHTTSEEQDEATVVHPREQSYLADDEPELFVTPPPVASFNPWR
                     IMIPAAVLLVVLLAAFFAFQRSDSSATNDNLQPLTADPNASPVQPGATPTGSGESNIV
                     PATGAPTPSATTPAQTSTGTGGTVANPNPTAEATPTATPERDDAQNSNTPEEEEEDEP
                     TPTPTPRRNPTPPPVRTPPVDDPPPPSPRATPPADLSSPADDPPPADNLL"
     gene            6074..>6924
                     /locus_tag="VGB76_05615"
     CDS             6074..>6924
                     /locus_tag="VGB76_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0098413.1"
                     /translation="MTKKQLLVVGGVAAGLAAAMQARRTAPALDITVLEKTADISYGA
                     CGLPYVVSGLIPALERLVIHTPEYFRAQHNIDIQLNTEALEILPARSIVRVGAGGERR
                     ELNFTSLVLATGAAAVCPPLPGHELAGVFVLRQLRDGRRLLQFIEQERPRKAVIVGAG
                     YIGLEMAEAFRARGLDTTLIESSDKVMSTLGGRLRERVVEELRVRGVEVFLGERVVAF
                     EGRERRIARVVTESGRTFEAQVVLIGVGVRPEVGIAKEAGLEIGESGAVATDAMQRTS
                     AANIYAAG"
BASE COUNT         1319 a         2143 c         2097 g         1365 t
ORIGIN      
        1 aagcccaccc tcacctggtc acagagacgc acgcgacgca ttctcggcca ccacgaccgc
       61 gccttcaaca ccatcgtcat cagcaagacg ctcgacgcgc ccgacgttcc cgagtggttc
      121 gtcgaatacg tcctgtatca cgagatgctc cacatcaagc atccggcgcg cgtcgtcaaa
      181 ggtcgccgct actcgcacac gaaagcattc ctcgccgaag aggaacgctt ccctctcttc
      241 gatcaggcgc aggaatggct cgaccatgtc gcgcgcgaac gccgcgccgc ctgaaaacga
      301 tccttctccg gccattatct tttttgaaac tttctcttcc gaccggcgtt ctaacagaca
      361 ggaaggtggg ctcctccgaa aagaacgggc gagcaacaag cacgcgctac cggacagtag
      421 catttgcagg ttgctcgccc gttctttgtt tactcatccg ccgccagttt tccgttcttc
      481 aatccaatct ttagtaactc gcgtccataa acatgaacga cggacaactg gctgccaacg
      541 gcggtcgagt tttcgacact tcgacacttc cttgacacgc ccaacctctc gtgatttcat
      601 gccttacaca tcgtcttaaa attcgatccg aaattctgta aggagattcc agatgggcga
      661 aagtgttttc aagggcgtag agatggtcgg gacatccgat caaagtttca gccacgccat
      721 cgaagtggca gttaaacgcg cacggcaatc gctgcgcaat ctctcctggt tcaccgtgaa
      781 ggagcagcgc ggcgggctgc aagacgggcg gcttgagtat caagtgacgc tcgaactctt
      841 ttttaaactg gaagccatgg agaacgatcc ggtggaagac tgagcgcact tctgcgcgcg
      901 tgctgcccgc acgtttcgcg gctggacaaa gggagtaaac gctcgctaga ctacgcagtt
      961 tgcgatacgg gtttattttc aaccctttca agatgcgtcg gcgtgcactg cacgcgcggg
     1021 cgaaatttat tcaagacgga gcaggataat ttcatggcga ttccagcaac ccaaatccgg
     1081 cgcgggatgg tgattctctt cgagggcaat ctctgcaaag tgatcgattt tcgacatcac
     1141 acgcccggca acctccgcgc gatggtgcag acgaaaatgc gcaacctgcg caccggctcg
     1201 tccttcgagc atcgcttccg ttcctccgac acggtggaca aagcctcgct cgaacagcat
     1261 gagatggaat acatgtactc ggacggttcg cagcaccatt tcatgaacac ggagaactac
     1321 gaacagaccg cgctcaccga agaagacctc ggcgacatgg cacaatggct catgccgggc
     1381 atgaagattc aagcggagtt ttacgaaggc gcgcccatcg gcatcacgct gccgccctcg
     1441 atggaactca ccgtgacgcg taccgaaccg acgctcaaag gcgcgaccgt ctccaacgtc
     1501 aacaaacccg cgacgctcga aaacggcgtg accatcaccg tcccgccctt cgtcaacgaa
     1561 ggcgaccgca tccgcgtcga cccgacggaa ggccgctaca tcgaacgagc caagtaaagt
     1621 gatgagtgtt gagtgacggg tgatgagtaa aagcattttg ttgcttctca ctcaacactc
     1681 aacactcaac actcatcact catgtacctt ctctacagcc ttcttctcac cttcgccatc
     1741 ttcgcgctgc tgcctttgtt tctcctcgat gcgttgcgtc atgggaaata cgtcgccggt
     1801 ttgcgcgagc ggctcggcgg tgtgccggag gttgacgcga acgggcgcgc tgttgtctgg
     1861 ttgcactgcg tctcggtcgg ggagacgcag gcggcacgac cgctggcgcg cgcgctcgtc
     1921 gaacgcttcc cttcgcacgc gctcgtcgtc tccaccacga cgctgaccgg acagacgctc
     1981 gcgcgcgaag tttttcgcga cgaggcggtc gccgtctttt actttccttt cgattgggcg
     2041 tggagcgtgc ggcgttccct gcgccgcatc aaaccttcgc tcgtgctcgt gatggaaact
     2101 gagctttggc cgcgtttttt gcgtgagtgc cgcagcgcag gcgtgcccgt cgcactcgtc
     2161 aacgggcgca tctccgaaaa gtcgtttcgc ggttatagaa aattgggagc ctttatcaaa
     2221 agcgtgacgg gcgcgctcac gctcgcgatc atgcagacgg aggccgacgc cgaaaggatt
     2281 cgcgcgctcg gactcgcgcc ggagcgaaca cgcgtctccg gcaacatcaa attcgacgcg
     2341 gacacggaag taggcgcggc agttatcacg cgtgaactac gcgagcgttt cgccttcggc
     2401 gacgaacgac cgctcatcgt ggccgccagc acacacgcgc cggaagaagc ggtcacgctc
     2461 gacgcattcc gacaggcacg cgccgccttc gacagagacg acagcagatg cccgcgcctg
     2521 ctcatcgcac cgcgccaccc cgaacgcttc aacgaagtcg ccgccctgct tgcatcgagc
     2581 ggcttatcgt gggcgcgtcg ttcagtttcg ccgaaggccg acgacgcgac gtgcgatgtc
     2641 gtccttctcg acaccatcgg cgaactacgg ggcgtctatt cgctcgccgc gctcgtcttc
     2701 gtcggcggca gcatcgcgcc gaccggcggg cacaacatac tcgaaccggc tgccgccgcc
     2761 tgttgcatca tcactggcgc gcacaccttc aatttcgctt ccatcatgcg cgattttctc
     2821 gaacaggatg cgctcgtcca attgcccacg ctcacggaag cgcaggctcc cgccgtgctg
     2881 gcacaaactt ttcgcgaact gttaaatgac aacgaacgcc gacgccgcac aggcgaccgc
     2941 gcacgcgccg cgctcgaaca tagtcgcggc gcaacggata agaccatcgc attgctcacg
     3001 ccactgttag aacagaggcc ggagagcaga ggttagagtt cagtcaagac aaaaactgct
     3061 ttctactaac ctctgctctc taatctctaa cctccgattt atgcttcggc tccgagacat
     3121 agcgttcgca ccgttgagtg tcgcctacgg cgcggcgggt cgtgcgcgtc gcgcgttata
     3181 tcgcgcgggc gtgctgcccg tcgaacgcgt agcggctccg gttgtgagcg tcggcaatat
     3241 cacggcgggc ggcacgggca aaacgccgct tgtcgaatgg ctggcgcgtg cggcggcgag
     3301 cgaggggcgt cgcgtctgca tcctgacgcg cggctacggg cgcgccgatg cgagcaagcg
     3361 ggtcgtcgtc tcggacggcg agcacgttat ggcggaggca cgtgagggag gcgacgagcc
     3421 gcgcctgctc gcggagaatt tactggagca caaggtcgcg gtggtgagcg acgcgaaccg
     3481 tgcggcggcg gcgcgttggg cgttggcaca cctgcgcagc gacgccttta ttctcgacga
     3541 tggatttcaa cacctgcgcc tctcgcgcga cctgaacatc ctgacgctcg atgcgaccga
     3601 cccctggggc ggcatgaaat tgcttccacg cgggcgtctg cgcgaaccgc ccggcgagtt
     3661 gaagcgagcc gatctaatcg tcatcacccg cgcggaactc gcagttgaca tcgaagaggt
     3721 gcgcgcgcgt gccgaggctt tgagcggcgg cagacgcccg gtgctcatcg cacgcaccgc
     3781 gacgctgcgt atgaagttac tcgccgttcc gcatagtgag ttgaaggtgc gtgcgcatga
     3841 gcacgagatc gagagcgttc cacaaacgct cgcggcgttc tgcgccatcg gcaacccgca
     3901 ggctttcttc gcacacgcgc gcagagacgg actcgacatc atacacgcgc gcgcgttcac
     3961 agaccatcac gtctacacgc agagcgatat agacctttac gtacgcgaag ccgtgcggca
     4021 aggcgcgcgt gcgttgctga cgacggccaa agacgccgtc aaacttcgcg cgctcaattt
     4081 tgcgctgccc tgttatgtgt tggaagtcgc gttgaagttc gacgatgagc agaagatgcg
     4141 cgccatcctc agagaagctc tcgcgcgccg cttgagcgaa ctataagagg ttgtcggcgg
     4201 gcggggggtc atccgccgga ctggatagat cggcgggcgg cgtcgctctc ggactcggcg
     4261 gcggcggatc atccaccggc ggagtacgca cggggggcgg cgtcgggttg cgtcgcggag
     4321 tgggagtagg cgtcggctca tcctcctctt cctcttccgg agtattgctg ttctgcgcat
     4381 cgtcacgctc gggcgtcgct gtaggtgtgg cctctgcggt cgggttcggg ttcgcaactg
     4441 tgcctcccgt gcccgtagat gtctgcgcgg gcgtggtcgc cgagggggta ggcgcgccgg
     4501 tggcggggac gatgttgctt tcgcctgagc ctgtcggcgt tgcacccggt tggacgggcg
     4561 aggcgttagg gtcggctgtc agcggctgca aattatcgtt cgtcgcagac gagtcggagc
     4621 gttggaaggc gaagaaggcc gcgagcagga cgaccagcaa gacagccgcc gggatcataa
     4681 tccgccacgg gttgaaactc gccacgggcg gcggcgtgac gaaaagttcg ggttcatcgt
     4741 ccgcaaggta tgactgctcg cgcgggtgca cgacggtcgc ttcgtcctgc tcttcgctcg
     4801 tcgtgtggcg cgattcgagc ggcgcggtcg gcacgacgat gcggttcgtg ttgcctgaac
     4861 ccaactgggc gttttcgctc gtctcgcctc cgaccgaagc tgtctggaca tcgggcgcat
     4921 ctgccgccgc gctcgccaac gcttcggcca gttctcccgc actcgcctga cgatcttccg
     4981 gcattttaga gagcgcacgc gcgatgacct gtgcgacagc gggcggcaca tccttccgct
     5041 cgtcgagcac cgaaggcggc ggctcctgta ggtgcttcaa catgatcgcc gtcgccgatt
     5101 cgcccgtgaa cgggacgtgg ccgacgagca tctcgtagag gatgatgcct aaactgtaga
     5161 tgtcggaacg cgcgtcaatg ccgctggcct gcgagcattg ttcgggcgac atgtattgcg
     5221 gcgtgccgat gatgagattg ggagcggtga gttcggggtc tggcccttcg gtttctaaaa
     5281 ttttggcgat gccgaagtcg agcactttag cccagtcgct gccgcccgcc acctcttcga
     5341 gcatgatgtt gtcgctcttg aggtcgcgat gcacgacgcc ttcggcatgc gccgcatcga
     5401 gcgcgccgca aatctggcgc gtgacttcga cgacgcgcgc gagcggcatc gggccttcgc
     5461 cccggatcac ttccttgagc gtcttcccgc gcaaatattc catcacgaga aagacgatgc
     5521 cattttccga ttcgccgaaa tccgtgacgt tcagtgcgtg ggggtttgag atgcgcgagg
     5581 cggctttggc ttcgcgtgtg aagcgcgcga cgatcttgtc gtcggcggcg agcgaagggt
     5641 gcagcacctt gatcgcgacg gccttctcca tcaaaacgtg cgtcgcacga tagaccgtgc
     5701 ccatgccgcc ttcgctgatg cgttcctcga tgcggtactt cgatgccaga gtctgtccga
     5761 ctaaagcgtc tccggtttta actaagaccg cgccgtctgc cgggcaaagc gtgggcgcgt
     5821 tctcataagt cttcccgcat ttcggacatt ttttcatcaa acgctttcgc ctcgcatcgc
     5881 gaattctgaa ggagtttctt aatcgaagcg tatcataccg ctacaaacgc cccaaggtaa
     5941 aactatacca cgcaaacctt aagccacacg cgcgaacacg tctcgccggt ggtttttctt
     6001 ttaacttttc aggaatctta agtaatcttg cccctgctcg ttatttcata tacgtgtgaa
     6061 ggcggctgaa cgtttgacta aaaaacagct actcgtcgtc ggcggagtgg ccgccggact
     6121 cgcggcggcc atgcaggcgc gtcgcaccgc gcccgcgctc gacatcaccg tgctggagaa
     6181 gaccgcagat atttcctacg gcgcgtgcgg cctcccttac gtcgtctccg gcctcatccc
     6241 cgcgctcgaa cggctcgtta ttcatacgcc cgaatatttt cgcgcgcagc acaacataga
     6301 cattcaattg aatacggaag cgttggagat tctgcccgca cgctcaatcg tacgcgtcgg
     6361 cgcgggcgga gagcggcgcg aactaaactt tacgagtctg gtgctggcga cgggcgcagc
     6421 agccgtctgc ccgccgctgc cgggacacga actcgcgggc gtcttcgtcc tgcgtcaact
     6481 gcgcgacggg cggcgactct tacagttcat cgaacaggaa cgtccacgca aggcagtcat
     6541 cgtcggcgcg ggctacatcg gccttgagat ggccgaagcc tttcgcgcac gcggactcga
     6601 cacgacgctc atcgagtcgt ccgacaaagt gatgagcacg ctcggaggcc gcctgcgcga
     6661 acgcgtggtt gaagaacttc gcgtgcgcgg cgtcgaagtc tttctgggcg agcgcgtggt
     6721 ggcttttgag ggacgcgagc gacgcatcgc gcgcgtcgtc acggagagcg ggcgcacgtt
     6781 cgaggcgcaa gtcgtgttga tcggcgtcgg cgtgcgcccc gaagtgggaa ttgcgaaaga
     6841 ggcggggttg gagatcggcg agagcggcgc ggtcgccacc gatgcgatgc agcgcacgag
     6901 cgcagccaac atctacgcgg cggg
//
LOCUS       DASWNS010000162         5810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_266053, whole genome shotgun sequence.
ACCESSION   DASWNS010000162 DASWNS010000000
VERSION     DASWNS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266053"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..427)
                     /locus_tag="VGB76_05620"
     CDS             complement(107..427)
                     /locus_tag="VGB76_05620"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0098414.1"
                     /translation="MDNRKEGERVKTALPCNWGLSEDSPRNGNITSLSQNGCFIQTKA
                     NATENQCVYINCWLPSERWLPLKGRIIYHLPRVGFGLTFLDLTDEQKQMISFLIEYHR
                     EEQG"
     gene            complement(508..891)
                     /locus_tag="VGB76_05625"
     CDS             complement(508..891)
                     /locus_tag="VGB76_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0098415.1"
                     /translation="MTKLTGLDLLFLEVNSLQDSVEFYRERLGFELQTLTEQAEPPMA
                     TLRAGRLRVMLAEQLLTMARRGRGVHLFFGVTDVEEYYAQLQARGLDLAPPSDEGWGG
                     RFVTLEDPDHYRLFFVTWEGDKSDE"
     gene            complement(894..1466)
                     /locus_tag="VGB76_05630"
     CDS             complement(894..1466)
                     /locus_tag="VGB76_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY0098416.1"
                     /translation="MGEAGERRDYYTVLGAERDDSRAEIERRYKRLAVEHHPDRGGDE
                     EEMKAINEAWGVLKDDATREAYDASYQSRGRIGEYVAPGFVPSTTPGAQADALGGRLA
                     GSMLSLFAGIVLIFLVRFHYVVFLWPLALLGLSLLLFGVWMAHAALAYAREGYAPEHP
                     ARRLIWAQEILFWSAIAGGGYVLYLLLTAI"
     gene            1670..2029
                     /locus_tag="VGB76_05635"
     CDS             1670..2029
                     /locus_tag="VGB76_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098417.1"
                     /translation="MALPFEPGDDLIFQLESGFGLLRVLAIEARDDETIWHLLAYEEF
                     FPDVESAEAALNNAAALGVRAAHLPLTDHAFGKTPAARLGNRPVTEDELAAYRAWSAK
                     GDAQPFDRSALLMLGLR"
     gene            2059..3768
                     /locus_tag="VGB76_05640"
     CDS             2059..3768
                     /locus_tag="VGB76_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0098418.1"
                     /translation="MNLQLKAMSSRRFRRVYFPALLLLSALCSFAQAERRERLIENWR
                     PLHYDVTLAFDDKLAELTRAETRLDALVLRDQLSVIDLDFGALTIESASVNGSPARYE
                     RPPQGERLNVFLARPARRGERLQLRIIYRGRPADGLILTDDKDGRPSATGDNWPNRAH
                     HWIPTLDHPSAKATVKFSVTAPAHTTVVANGRLESRRTNANATQTWTYSEAAPMPPYC
                     MVVAVGQFAELKPGVVLPATLPPVAYYVPPSEQKFALEGFGAAVPSLTFFSQTVAPFP
                     YAKLAHIVGATRFGGMENSGAIIYSRTLFDPRENAPVSPRFRVRRGLVELIAHETAHQ
                     WFGDAVTPATWADLWLSEGFATYYAGLFVERHEGVAAFREYMERKGGEYLKYEQTRRA
                     PIYDRETEDLNKLLNANNYQKGAWVLHMLRAELGDASFFRGIRAYYRAHRHKTATTED
                     LRRALERASGRSLREFFARWVYASGHPRYEAAWTWNAGTTAAARGAGVLTLTLKQTQE
                     DGAPFPNAFPVEIVTGNGTRRVKLTPNGRETSQRIYLNRRPSEVRFDPDATILKEISV
                     RPS"
     gene            complement(3973..>5810)
                     /locus_tag="VGB76_05645"
     CDS             complement(3973..>5810)
                     /locus_tag="VGB76_05645"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEY0098419.1"
                     /translation="NAATNLTGEAAANVKKVFAYGIRNSFGMAFDPQSGQLWTQENGD
                     DSFDEINRVVPAFNGGWIQMMGPSARVAEFKAIELGRSGGLQQIRWPPDRIADTPAEA
                     LARLYMLPGAIYTEPEFSWKYAVAPSPVGFINGRALGANNEGALVVGASRTTLLGGYL
                     FRFKLASDRRKLSFTDARLSADLVADNADKFDINESESLLFGKDFGITTDIQTAPGGN
                     LYVVSLSDGAIYEISSKPSTIYFTPQESVVTESNTSLSVSVTRSGDTSGTVSVDYRTI
                     DDPAAVPCDPTRRKPDGTSYPQGTAYARCDYATAAGTLTFAPGETSKSFSVSIIDDGH
                     AETLETFNVELLNPAGATLDVHTAKPISINDNDAANAPNPIYETFTGNFFVRQQYLDF
                     LSREPDPAGQESWLRVLRDCSDVHNNPVCDRITVSASFFRSREFQLKGLFVYLFYQVS
                     LGRLPRYTEIIPDMASVTGATAEEVFAKRDAFANNWLARPEFKTLYPGTLTPAEYVDK
                     LLQTTQVTLNAAPTRDTLIADLQTGRKTRAEVLRAIVEHPDVNAKEFNEAFVAMQYFG
                     YLRRDPDAQGYQNWLNYLNANPTDFRTMVRGFLNSNEYLLRFGKV"
BASE COUNT         1079 a         1810 c         1784 g         1137 t
ORIGIN      
        1 tttgagggat gagggatgaa ggatgaagga tgaatcagaa aaagcactca gtcgcttgaa
       61 tgcttcttct tattcatcct tcatccttca tccctcatcc cttaattcat ccttgctctt
      121 cgcggtggta ttcgatgagg aaagagatca tctgtttctg ttcgtccgtg aggtcgagaa
      181 aggtgaggcc gaagccgact ctggggaggt gatagatgat tctgccctta agcgggagcc
      241 agcgttcact cggcagccag cagttgatat agacacactg gttctctgtg gcgttggctt
      301 tggtttgaat gaaacagccg ttctggctca ggctcgtgat attcccgttg cgcgggctgt
      361 cctcgctcaa cccccagtta cacggcagcg cggttttcac gcgctctccc tcttttcgat
      421 tatccataag gcgtgaagtg atgagtgacg ggtgatgagt gatgagctaa gacaacagcc
      481 ttgtcttagc tcatcactca tcacccatca ctcatcactt ttatctcctt cccacgtgac
      541 gaagaagaga cggtaatgat ccgggtcttc gagggtgacg aagcgaccgc cccagccttc
      601 gtcgctcggc ggggcgagat cgagtccgcg cgcttgcagt tgcgcgtaat actcttcaac
      661 gtccgtgacg ccgaaaaaaa gatgcacgcc gcgcccgcgc cgcgccatcg tcaggagttg
      721 ctcggccagc atcacgcgca gacgcccggc acgcagcgtc gccatcggcg gctcggcttg
      781 ctccgtcagc gtctgcaatt cgaagcccag acgttcgcgg taaaactcca cagagtcctg
      841 caaactgttg acttcgagaa acagcagatc gagtccggtt aatttcgtca tcgttaaatt
      901 gctgtgagta agaggtagag cacgtagccg ccgcccgcga tggcagacca gaagagaatc
      961 tcctgcgccc agatgagacg ccgcgccggg tgctccgggg cgtatccttc acgcgcatag
     1021 gcaagcgcgg cgtgcgccat ccacacgccg aagagcagca gcgacagccc gaggagcgcg
     1081 agcggccaca ggaacaccac gtaatgaaag cgcactaaaa agatcagcac tattccggcg
     1141 aacaggctca acatcgaacc tgcgagccgc ccgccgagcg catccgcctg cgctcccggc
     1201 gtcgtcgaag ggacaaaccc gggcgcgacg tattcgccga tgcgcccacg gctttgatac
     1261 gacgcatcgt aagcctcgcg cgtcgcgtcg tctttgagca cgccccacgc ttcgttgatc
     1321 gccttcatct cttcctcgtc gccgcccctg tccgggtgat gctcgacggc gagccgttta
     1381 taacgccgct cgatctccgc gcgtgaatca tcgcgctccg cgccgagcac cgtgtaatag
     1441 tcgcgcctct cgccagcctc gcccatgccc tcaccttcgc ccggagtgtg ccgaatagac
     1501 taatagtaac cgtcctgcgc gcgggagggc aaaggataag gggtagaggc gaggggtgaa
     1561 gatgaaactc ttcgtgtgcc cgtctacctt ttccctctct cccttaccct ttccccttct
     1621 ctctttgtgt taagctagcg cacatctcaa cgaatgcggg tgaacgatta tggctttgcc
     1681 tttcgagccg ggcgacgatc tcatctttca acttgagagc ggcttcgggc tgctgcgcgt
     1741 ccttgccatc gaagcgcgcg acgacgagac gatctggcat ctgctcgcct acgaagaatt
     1801 tttccccgac gtggaaagcg ccgaagccgc gctcaacaat gccgctgcac tcggcgtccg
     1861 cgcggcgcac ctgccgctca cagatcatgc gttcggtaag actcccgccg cgcgtctcgg
     1921 caatcgcccc gtgacggagg acgaactcgc cgcctaccgc gcgtggagcg cgaagggcga
     1981 cgcccaaccc ttcgaccgtt ccgccctcct gatgctcggg ctgcgctgaa cgatctttgg
     2041 attgagaaac ttaagccgat gaatcttcaa cttaaagcga tgtcgagccg ccgtttccgg
     2101 cgcgtttact tccccgccct gctgctcctg tccgcgctct gttccttcgc gcaggccgaa
     2161 cgccgcgagc gtctcatcga aaactggcgt cctctccact acgacgtgac gctcgccttc
     2221 gacgacaaac tcgccgaact tacacgcgcc gagacgcgtc tggatgcgct cgtcctgcgc
     2281 gatcaactga gcgtcattga tctggacttc ggcgcgctca cgattgaatc cgcgagcgtc
     2341 aacggcagcc cggcgcgcta cgaacgaccg ccgcagggcg agcggctcaa cgtttttctc
     2401 gcgcgccccg cgcgtcgcgg cgaacggctg caactccgca tcatctatcg tggacgaccc
     2461 gccgacggcc tgatcctgac agacgataag gacggtcgcc cctccgccac cggcgacaac
     2521 tggccgaacc gcgcgcacca ttggataccc acgctcgatc acccgtccgc gaaagcgacc
     2581 gtcaaattca gcgtcaccgc gcccgcgcac accacggtcg tcgccaacgg gcgtctggag
     2641 tcgcgccgca ccaacgcgaa cgcgacgcag acatggactt acagcgaagc cgcgccgatg
     2701 cctccctact gcatggtcgt cgccgtcggc cagttcgccg aactcaaacc gggcgtggtg
     2761 ctccctgcga cgcttccgcc cgtcgcctat tacgtgccgc cttcggagca aaagttcgcg
     2821 ctcgaagggt tcggcgcggc tgtcccgtcg ctcaccttct tcagccagac cgttgcgcct
     2881 tttccttacg cgaagctcgc gcacatcgtc ggcgcgacgc gcttcggcgg catggaaaac
     2941 tccggcgcga tcatctattc gcgcacgctc ttcgacccgc gtgagaatgc ccctgtgagc
     3001 ccgcgtttcc gcgtccgtcg cggtctggtc gaactcatcg cgcacgagac ggcgcaccag
     3061 tggttcggcg acgccgttac gcccgccacc tgggcagacc tctggttgag cgaaggcttc
     3121 gccacttact acgcaggtct tttcgtcgaa cggcatgaag gcgtcgccgc ctttcgcgag
     3181 tacatggagc gcaaaggcgg ggaatactta aagtatgaac agacgcgccg cgcgcccatc
     3241 tacgaccgcg agacggaaga cttgaacaaa ctcctgaacg cgaacaacta ccagaagggc
     3301 gcgtgggtct tgcacatgct ccgcgccgaa ctcggcgacg cgtccttctt tcgcggcata
     3361 cgcgcctact accgcgcgca ccggcacaaa actgccacga ccgaagattt acgccgcgcg
     3421 ctcgaacgcg cttccggcag aagcctgcgc gagttcttcg cgcgctgggt ttatgcgagc
     3481 ggccacccgc gctacgaagc cgcctggacc tggaacgcag gcacgacagc ggcggcgcgc
     3541 ggcgcgggcg tgttgacgct cacgctcaag cagacgcagg aggacggcgc gccgttcccg
     3601 aatgctttcc ccgtcgagat cgtgactggc aacggcacac gccgcgttaa gctcactccg
     3661 aacggacgcg aaaccagcca gcgtatctac ctgaacaggc gaccgtcaga ggtgcgtttc
     3721 gaccccgacg cgacgatact caaagagatc agcgttcgac cttcctgaaa atactttgac
     3781 gctctgctcc agcgtgcccg ccggagacat tcgccgcaga ggcgcgagag cggagcgcga
     3841 agagagccaa ggccggatca ctcaagggcg cggagagttg aatgatcaac tctccgcgcc
     3901 cttccctgat ccggctcgcg cgtctctgcg gcgaagtttt tgtcggagtt tgagatcggt
     3961 gctgccggag cgtcagacct tgccgaagcg caggaggtac tcgtttgagt tgaggaaacc
     4021 tctgaccatc gtgcggaagt ccgtcgggtt ggcgttgagg tagttcaacc aattctggta
     4081 gccctgcgcg tccgggtcgc gccgtagata gccgaagtat tgcatggcga cgaacgcttc
     4141 gttgaactcc ttcgcgttta cgtccgggtg ttcgacgatg gcgcgcaaga cttccgcacg
     4201 cgtcttcctg cccgtctgca agtcggcgat cagtgtgtcg cgcgtgggcg cggcgttcaa
     4261 agtcacctgc gtcgtctgga gcaatttgtc cacatactct gcgggtgtca gcgtgccggg
     4321 gtagagcgtt ttgaattcgg ggcgcgcgag ccagttgttg gcgaaggcgt cccgcttggc
     4381 gaagacctct tcggcggtcg cgcccgtgac gctcgccatg tccggaatga tctccgtgta
     4441 gcgcgggagg cgaccgagcg agacttgata gaagagatag acgaataagc ccttcaactg
     4501 aaactcgcgc gagcgaaaga aactcgcaga gacggtgatg cggtcgcaca cagggttgtt
     4561 gtgtacgtcg gaacaatctc tgagcacgcg cagccacgat tcctgtcccg ccggatcggg
     4621 ttcgcgcgag aggaaatcga ggtattgctg gcgcacgaag aagttgccgg tgaaagtctc
     4681 gtagatgggg ttgggcgcgt tggcggcgtc gttatcgttg atgctgatcg gcttggcggt
     4741 gtgaacgtcg agcgtcgcac cggcggggtt gagcagttcg acgttgaacg tctcaagcgt
     4801 ttcggcgtgc ccgtcgtcaa tgatcgagac ggagaaactc ttgctcgttt cgccgggggc
     4861 gaacgtgagc gttccggcgg cggtggcata gtcgcaacgc gcgtaggccg tgccctgcgg
     4921 gtaagaggtg ccgtcgggtt tgcggcgcgt cgggtcgcag ggcacggcag ccggatcgtc
     4981 aatcgtgcgg taatcgacgg agacggtgcc gctcgtgtcg ccgctgcgcg tgacagagac
     5041 ggagaggctc gtgttgcttt ccgtcacgac cgactcctgc ggagtgaaat agatcgtgct
     5101 cggcttcgag gagatttcat aaatcgcgcc gtcggagagc gagacgacgt agaggttgcc
     5161 gccgggcgcg gtctgaatgt cggtggtgat gccgaagtct ttgccgaaga gcaggctttc
     5221 gctttcgttg atgtcgaact tgtctgcgtt gtcggcgacg aggtctgcgc tgaggcgcgc
     5281 gtctgtgaag gagagtttgc ggcggtcgct ggcgagtttg aagcggaaga gataaccgcc
     5341 gaggagcgtg gtgcgcgatg cgccgacgac gagcgcgcct tcgttgtttg cgccgagcgc
     5401 gcgcccgttg atgaagccga cgggcgacgg cgcgacggcg tacttccaac tgaactccgg
     5461 ctccgtgtag atcgcgcccg gcaacatgta gaggcgcgcg agggcttccg ccggagtgtc
     5521 tgcgatgcgg tcgggcggcc agcgaatctg ctggaggccg ccgctgcgcc ccagttcaat
     5581 cgctttgaac tcggcgacgc gggcggaggg tcccatcatt tgaatccagc cgccgttgaa
     5641 ggcgggcacg acgcggttga tctcgtcgaa gctgtcgtcg ccgttctcct gcgtccagag
     5701 ttggccggac tgcggatcga aggccatgcc gaaactgttg cggataccgt aggcgaaaac
     5761 ttttttgacg ttggctgccg cctcgcccgt gagattcgtc gcggcgttga
//
LOCUS       DASWNS010000163        10118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_317361, whole genome shotgun sequence.
ACCESSION   DASWNS010000163 DASWNS010000000
VERSION     DASWNS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317361"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1157
                     /gene="hprK"
                     /locus_tag="VGB76_05650"
     CDS             87..1157
                     /gene="hprK"
                     /locus_tag="VGB76_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEY0098420.1"
                     /translation="MNDTGATQPSINVGDFIKHAPPQLELRVLAGSRGLHARRITSAR
                     IQKLGLALSGFTHYIHAGRVQIIGQSEVQFLGQLTPARRREAIDNLSLETISCVLVTK
                     EQTPPVEFVEAAESAELPLLQTTLVSSVAIGVVNDYLRETLAPRELRHGVLLDAYGLG
                     VLIEGDSGIGKSECALDLIVRGHRLVADDLVEVRRLGADYLLGSAPELLREHMEIRGL
                     GIINVRDLYGVAAISGPKQIGLSIRLERWKDAREVERLGIDERTVDILGVSVPHVLLP
                     VSPGRNLSTLIETALRVHLLRLRGYNAAERFVARHNEMLGTDAGGVREEPSSRDAVDA
                     PTPGEPAPERERTIDEPEKKRG"
     gene            1132..2106
                     /gene="rapZ"
                     /locus_tag="VGB76_05655"
     CDS             1132..2106
                     /gene="rapZ"
                     /locus_tag="VGB76_05655"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY0098421.1"
                     /translation="MSRKKRGANRRTASAPDIVVITGLSGSGMSSATNAFEDLGYFCV
                     DNLPLTLLPTFARLVQPEDEEVEDTEARIKRAALVINIREGHFLSEFERQLKKLRRAS
                     GLEVFVLFFEASDEVLQRRFSETRRPHPADTGDGLLASIRAERAAMADIRAHADHIIN
                     TSDHSVHTLRRYLIERFSPDAESTTMRVQVLSFGHKYGGPRDLDLLFDVRHLPNPYFV
                     AELRDFSGKDRRVADFLKKQPETIETLTRFGELLDYLLPLYQREGKSYVTVGVGCTGG
                     RHRSVMVANALARRLRRDGFNATAVHRDVSKAKPRRRAARKSDRAAST"
     gene            2144..2458
                     /locus_tag="VGB76_05660"
     CDS             2144..2458
                     /locus_tag="VGB76_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY0098422.1"
                     /translation="MKEHDDCIRCRHDYRLEDVSIEHEFIICTDCTQHLNPSRETVRV
                     CPIDGREMKKLIIYDLVLIDKCAACGGIWLDQNELKLIARIVGVNAAVGGYLMGNIAN
                     DL"
     gene            2491..2937
                     /locus_tag="VGB76_05665"
     CDS             2491..2937
                     /locus_tag="VGB76_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY0098423.1"
                     /translation="MDEGGKVETKRRVAGVVVTHGQLATELLAAAEMIIGPIAHIAAI
                     SIGWHDDVDVARDEIEAAIARVSEGGGVLILTDMFGGTPTNIASMFFVEGEVEVVTGV
                     NLPMVIKLASQTEAEETLAVVAGRVRDQGQQAIYLAGELLAAPKKS"
     gene            3019..3300
                     /locus_tag="VGB76_05670"
     CDS             3019..3300
                     /locus_tag="VGB76_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY0098424.1"
                     /translation="MLEKRILIVNRLGLHARAAARLVRTASAFRSSVRLERTDGSAVA
                     DAKSILSVLLLAAARGTELRLTVEGADEREASDAVCALVESGFGEEVES"
     gene            3297..5117
                     /gene="ptsP"
                     /locus_tag="VGB76_05675"
     CDS             3297..5117
                     /gene="ptsP"
                     /locus_tag="VGB76_05675"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY0098425.1"
                     /translation="MSSRVKNGKREMRWRGVAVSEGVAIGRVLRIHTGGRQNIYRATL
                     EADDIKREVRRFQAAVRLSRRQLLAIKRRAEVALGAEHAYIFDAQLLMLEDRKLLDEV
                     EDYIRQERANAEWAVKVASDKLLAVYAQIKDDYLRERSSDIEDVTRRLLVALSGEAQS
                     RRLTEDAIIVAEELLPSAAAELDFSHVRAIASDAGGWTSHTAIIARGLGIPAIVGLRE
                     LYRHARTGDEIVIDAERGEVVLHPATGTLAHFREARKVVSASAAGEGAADGGREPLLT
                     TDGVEIILRANVELPPEYEGVRRYGARGIGLFRSEFLFSQRGGAPTEDEQCAAYEEIA
                     ALAGAEGAKVRLFDVGGDKHSGVGGSGSGDFEIERNPALGLRAIRFSFRHEEMLRTQV
                     RALLRASARGRLDVVLPMVSDVSEVRHARRIIEEERGRLQAEGNETGELKLGAMIEVP
                     AAVLVADKLAREVDFFSLGTNDLVQYLLAVDRGNDEVSAWFRTLHPAVLQSIQRTIEA
                     AGGAGIPAIVCGEMAATPAYALVLVGLGARVLSMTASAMPRVRRTLRRIDASSANEIA
                     ALCLNAATADDAEETVRVELGARWPHLFSPENLPPVKGEE"
     gene            5531..6826
                     /locus_tag="VGB76_05680"
     CDS             5531..6826
                     /locus_tag="VGB76_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0098426.1"
                     /translation="MSKVLFAFVIGLAFSVPLAATAQAQSRLGPSEDDTHAASTPTPA
                     AKSDAKKSDAKKEAPKTGSPVVAAPPKPAPAGEARRDEAKDDRAKSEAQKTPTVLSSA
                     VAPNTNLPASTPNTTSDGAPKTSNSPLPVFGSPSVRGIPAPPRYEPPAAVKATGEPAA
                     TTASTPPATTPNASPATTAAASLPPTALYRVGVGDILDIRLAGGMSRDSTLFTVMSTG
                     MLDYPLAGEPINVVGMTTDEIGARLSAELKRRAVYDRAQFRINVREYASHTATVSGLV
                     DQPGAKVLRREAVPLYVVLSEANPRAEAGRAVIISRATGRSKTLDLADSAALNELISH
                     GDIINITTRPQEFFYIGGQINAPGQKDFHPGMTLTQALLASGGVARDAGNKVKVTVSR
                     QGADGRLVATEYLLNDIETGQIPDPRLQAGDRVEVGRRR"
     gene            7033..9477
                     /locus_tag="VGB76_05685"
     CDS             7033..9477
                     /locus_tag="VGB76_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0098427.1"
                     /translation="MMQHKLSAFALAAALLCAPFTSVLAAAPAAAAPLASRAAVGAQQ
                     DDTPPDVEQAMFTKGQNFYNQGRFEQAATTFRDFLKNYPNSIITDLTLLWLGRTYIAQ
                     SRLSDAESVVARLRTIKDTPFVEIYEGELQAARAEMASRGVTAQPAATPSATTERAQA
                     TSNRPAVRVPAATPTPTPVARLVGRSSAPRTRPTPLPTPLPTPSATPQIARATPTPNR
                     TPTLRIENTPNANTQTNAPVVRPSRRNTRASRRAQPQRANTDTQVATNTPAVLPRPTP
                     TPIRPRQQAAAVATPTPIQLARAEPNDSVTSAPPIVNSAPSGQEGFVLTVKQVPNLNL
                     ALSRAALAASPGQVVQLPLTVTNTGNKEDQFRLGTDLPAEYQPTFSLASGGQDTGLPI
                     LVTPQLARGQNLEVMLNLRIPETALDGQQRPFIVSAASQSDYQIQRVTNAALTVVAAS
                     LAGSSGVTRESVQPGETFTQTISVRNTGSAPARSARADFVFSTDFELVTASPLPLYYD
                     NPSRTAIWSLGDLDSRATREITVTLRAVPNALAATAPIGRGMLRTASLPTGSNYDGPS
                     IAIGRVPRARIDPVSPGLTVTPGDVIFIPFVVRNPSNYAEAYGLRITAPGAPSGTLYA
                     DSNGDGQHQDSEPSVTQTGQIDPRGGQYPLLMRVEIPRSTADRQQYAYNVVASSLSSN
                     RAASEASTVLTVAAPRVRVRTEQVTDTTAPGDMIFYRLVLINEGAGLAKNIVVNEVLP
                     EALQFVNSNPSLNSQDAPGDSRRVTWRVPELAPNDTAVLLITVRLRPDLAADGTITTS
                     HTWVYQDTNGNTYQNQ"
     gene            9564..>10118
                     /locus_tag="VGB76_05690"
     CDS             9564..>10118
                     /locus_tag="VGB76_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098428.1"
                     /translation="MRGYSPFTTDAFVSAPATQAPATTPATRLTRLSRALIAKSIVEA
                     LFVVALAVYFSYTNINPFFRGSIDVADARTIEGWVVNEAAPDERVEVHLYIDEQFVSR
                     RSADAPRADVLAAGRSRDAYHGFIFATPPLPPGRIYHARVYAMHIAGDGALRVLQEIG
                     AATAFSVTADEESVHVPDDWRESEG"
BASE COUNT         1934 a         3322 c         3186 g         1676 t
ORIGIN      
        1 ggcgacatgg tcgagatcga actcaacatc atcacgcgcg cggcgagcgc cgaaggaact
       61 gctcccgccg ccacccccgc tgcgcaatga acgacacggg cgcaacgcag ccctcgatca
      121 acgtcggcga cttcatcaag cacgcgccgc cgcagcttga gttgcgcgtg ctggcgggca
      181 gtcgtgggct gcacgcgcgc cggataactt cggcgcgcat tcaaaaactc ggcctcgcgc
      241 tctccggctt cacgcactac atacacgccg ggcgcgtgca gatcatcggc cagagcgaag
      301 tccagtttct cggccaactg acacccgcac ggcggcgcga ggccattgac aatctctctc
      361 tggagacgat cagttgcgtg ctcgtcacca aggaacagac gccgcccgtc gaattcgtgg
      421 aagccgccga gagcgcggag ttgccgctgt tgcaaaccac gctcgtaagt tccgtcgcca
      481 tcggcgtcgt gaacgactac ctgcgcgaga cgctcgcgcc gcgcgagttg cgccacggcg
      541 tgctcctcga tgcctacggc ctcggcgtgc tcatcgaagg cgattcgggg atcggtaaat
      601 ccgaatgcgc gctcgacctc atcgtgcgcg gccaccgcct cgtcgcagac gatctcgtgg
      661 aggtgcgacg cctcggcgca gactacctgc tggggagcgc gccggaactt ctgcgcgagc
      721 acatggagat acgcggcctt ggcatcatca acgtccgcga cctctacggc gtggccgcca
      781 tcagcgggcc gaaacagatc ggcctctcca tccggctcga acgctggaag gacgcacgcg
      841 aagttgagcg tctcggaatt gacgagcgca cggtggacat tctgggcgtg agcgtgcccc
      901 acgttttgtt gcccgtcagt cccggcagaa atctctcgac gctcatcgag actgcgctgc
      961 gcgttcactt gttacgcctg cgcggttaca atgcagccga gaggttcgtc gcgcgccaca
     1021 atgagatgct cggaacagac gcgggcggcg tgcgggagga accttcaagc cgagacgcgg
     1081 tagatgcgcc gacgccggga gagcctgcgc cggagcgaga acgcaccatt gatgagccgg
     1141 aaaaaaagag gggctaaccg ccgcacggcg agcgcgcccg acatcgtcgt catcacgggc
     1201 ttgagcggtt cggggatgtc ttcggcgacg aacgcttttg aagacctcgg ctatttctgc
     1261 gtggacaatc tgcccttgac gctcttgccg actttcgccc gcctcgtgca gccggaagac
     1321 gaggaagtgg aggacacgga ggcgcgcatc aaacgcgccg cgctcgtcat caacatccgc
     1381 gaaggccatt tcctgagcga gttcgagcgg cagttgaaga agctcaggcg cgcgagcggc
     1441 ctcgaagtct tcgtcctctt cttcgaagcg tcggatgaag tcttgcagcg acgtttcagt
     1501 gagacgcgcc gcccgcatcc ggcggacacg ggcgacggcc tgctcgcctc catccgcgcc
     1561 gaacgcgccg ccatggccga catacgcgct cacgcagacc acatcatcaa tacctcagac
     1621 cacagcgtcc acacgttgcg ccgttacctg atcgagcgtt tcagccccga cgccgaaagc
     1681 acgacgatgc gcgtgcaggt cttgagcttc ggccacaagt acggcggacc acgcgatctc
     1741 gatctcctgt tcgacgtgag gcatctgccg aatccctatt tcgtcgccga gttgcgcgac
     1801 ttctccggca aggacaggcg cgtcgccgac tttctgaaaa agcagccgga gacaatcgaa
     1861 acgctcacac gcttcggcga gttgctcgac tacctgttgc cgctctatca acgcgaaggg
     1921 aagtcttacg tcacggtcgg cgtcggctgc acgggcgggc ggcatcgttc cgtgatggtc
     1981 gcgaacgcgc tcgcgcgccg cctgcgccgc gacgggttca acgctaccgc cgtccaccgc
     2041 gacgtgagca aagcgaagcc gcgccgtcgc gccgcgcgaa agagcgaccg cgccgcgtcc
     2101 acttgattcg ttcggacgtt tgataccgga gggaggaacc gggatgaagg aacacgacga
     2161 ctgcatccgt tgtcgccacg actacaggct cgaagatgtc tccatcgagc acgagttcat
     2221 catctgcacg gactgtacgc agcacctcaa cccgtcgcgc gagacggtgc gggtctgccc
     2281 gattgacggg cgcgagatga aaaaattgat catctacgat ctcgtcctga tcgataaatg
     2341 cgcggcctgc ggcggcatct ggctcgacca gaacgaactg aaactcatcg cgcgcatcgt
     2401 cggcgtcaat gcggcggtcg gcggttatct gatgggcaac atcgcgaacg atctttagcg
     2461 gcaatccgaa aaccggacgg agcgtgtggt atggatgagg gcgggaaagt tgagacgaag
     2521 cggcgcgtgg cgggcgtggt ggtgacacac ggccaactgg cgaccgaact cctcgcggcg
     2581 gcggagatga tcatcggacc catcgcgcac atcgccgcga tctccatcgg ctggcacgac
     2641 gacgtggacg tggcgcgcga cgagatcgaa gcggccatcg cgcgcgtctc ggaaggcgga
     2701 ggcgtgctga tcctgacgga catgttcggc ggcacgccga ccaacatcgc ttcgatgttc
     2761 ttcgtcgaag gcgaagtcga agtcgtgacg ggcgtgaatt tgccgatggt catcaaactg
     2821 gcgagccaga cggaggcgga agagacgctc gcggtcgtcg ctgggcgcgt gcgcgatcag
     2881 ggacaacagg ccatctatct cgcgggcgaa ctgctcgccg cgccgaagaa gagttgagtc
     2941 gaagcatcat cagaagagtt gagttgaagc attatctggt tgaagccgcg tcgggttgca
     3001 ggtcgaagcg aggcggaaat gttggagaag cgaatcttga tcgtgaatcg tctggggctg
     3061 catgcgcgtg ccgccgcacg actcgtgcgc acggcgagcg cgttccggag cagcgtgcgt
     3121 ctggagcgca cggacggaag cgcggtggcc gatgccaagt ctattttgag cgtgctgctg
     3181 ttggccgccg cgcgtggaac tgaattgcgc ctgacggtcg aaggcgcaga cgagcgtgaa
     3241 gcgtcggacg cggtgtgcgc gctcgtcgag agcgggttcg gggaagaagt ggaatcgtga
     3301 gtagtcgtgt gaaaaatggg aagcgcgaaa tgcgctggcg tggcgtggcc gtctcggagg
     3361 gagtggcgat agggcgcgtg ctgcgcatcc acacgggcgg gcggcaaaat atttatcgcg
     3421 ctacgctcga agccgacgac atcaaacgcg aggtgcgacg ctttcaagcg gcggtgcgcc
     3481 tgtcgcgcag gcagttgctc gcaatcaaac gtcgcgccga agtcgcgctc ggcgcggagc
     3541 acgcctacat cttcgacgcg caactcctga tgctcgaaga tcgcaagctg ctcgacgaag
     3601 tggaagacta cattcgacag gaacgcgcca acgccgagtg ggctgtgaaa gtcgcttccg
     3661 acaaactgct cgccgtctac gcacagatca aagacgacta cctgcgcgag cgaagttccg
     3721 acatcgaaga cgtaacgcgc cgcctgctcg tcgccctgag cggcgaagcg caatcgcggc
     3781 gtctcaccga agacgccatc atcgtcgcgg aagaactgtt gccgtccgct gcggctgaac
     3841 tcgacttctc acacgtccgc gccatcgcct cggacgcggg cgggtggacc tcacacacgg
     3901 ccatcatcgc gcgcggcctc ggcatcccgg ccatcgtcgg cctgcgcgaa ctctaccggc
     3961 acgcgcgcac gggcgacgag atcgtcattg atgcggagcg cggcgaagtg gtgctgcacc
     4021 cggcgacagg gacgctcgcg catttcaggg aagcgcgaaa ggtcgtgtcg gcgagcgcgg
     4081 cgggagaggg cgcggcggac ggcgggcgcg agcctctgct gacgacggac ggggttgaga
     4141 tcatcctgcg cgcaaacgtc gagttgccgc cggaatacga gggcgtgcgt cgttacggag
     4201 cgcgcggcat cggcctgttt cgttcggagt ttctcttctc gcaacgcggc ggcgcgccga
     4261 cggaggacga acagtgtgcg gcctacgaag agatcgccgc gctggcggga gcggagggcg
     4321 cgaaggtgcg cctcttcgat gtcggcggcg acaagcacag cggcgtcggc ggcagcggca
     4381 gcggcgactt cgagatcgag cgaaatcccg cgctcggcct gcgcgccatc cgtttctctt
     4441 tccgccacga agagatgctg cgcacgcaag tccgtgcgtt gctgcgcgcg tcggcgcgcg
     4501 ggcggctcga cgtggtgctg ccgatggtgt cggacgtgtc ggaggtgcgc cacgcgcggc
     4561 gcatcatcga ggaggagcgc gggcgtttgc aggcggaagg gaacgagacg ggcgaactca
     4621 agctcggcgc gatgatcgaa gtgcctgccg ccgttctggt ggccgacaaa ctcgcgcgcg
     4681 aagtggattt cttcagtctc ggcacgaatg atctcgtgca atacctgctc gcagtggatc
     4741 gcggcaacga cgaagtctca gcgtggtttc gcacgctgca tccggcggtg ctgcaaagta
     4801 ttcagaggac catcgaggcg gcgggcggcg caggaattcc ggccatcgtc tgcggcgaga
     4861 tggcggccac gcctgcttac gcactggtac tcgtcggcct cggcgcgcgc gtgctgagca
     4921 tgacggcttc ggcgatgccg cgcgtgcgcc ggacgctacg gcgcattgac gcgtcgagcg
     4981 cgaacgagat cgccgccctc tgcctcaatg ctgcgaccgc cgacgatgcc gaggagacgg
     5041 tgcgcgtcga actcggcgcg cgctggccgc acctgttttc gccggaaaac ttgccccccg
     5101 tgaaaggcga agagtagaat ctggcggtgt gccccgacaa tggcggcagc gtgcggcgac
     5161 cctttaaaac ttccgccaaa ctcgcttgtt cctcgcattc cgagacgata tttagcagcc
     5221 acttcgagcg aaactactcg aagacgagcg ggcgcacgcc gcgtttcagg taagatttga
     5281 gtttgacagg aaaaattgac aatttgaact ttgcgaaaaa gggcgttttc gtcgtttaat
     5341 ctaaaggacg ggacgcgcaa gtctcggcct ccgacccgcc cgtcggaggc aggcacgaca
     5401 ggcgcagcct cttcgccttc tgaagttttg gaattttgta gagacacagg gattttgacg
     5461 cggcgatttt taacggccac tgccccggcc aatgcgcccg cgtcgaagtt gcagggaagt
     5521 agagtgcaat atgagtaaag ttttatttgc ctttgtcatc gggctggcgt tcagcgtgcc
     5581 gttggcggca acggcgcagg cgcaatcacg gctcggcccc tcggaagacg atacgcacgc
     5641 cgccagcaca ccgacaccgg cggcaaagag cgacgcgaaa aagagcgacg cgaaaaaaga
     5701 ggctccgaaa acaggctcgc ccgtcgttgc cgcgccgccc aagcccgcgc ccgcaggcga
     5761 agccagacgc gacgaggcga aagacgaccg cgcgaaatcc gaagcgcaaa aaactccgac
     5821 cgtcctgtcg tccgccgtcg ctccgaacac gaaccttcct gcttcaaccc cgaacacgac
     5881 cagcgacggt gcgccgaaaa cctcgaactc gcctttgcct gtcttcggca gtccttctgt
     5941 gcggggcatc cccgcgccac cgaggtatga gccgcccgca gccgtcaagg cgacggggga
     6001 accggctgcg acgacagcct caactccgcc cgccaccacg ccaaacgctt ctccggcaac
     6061 tactgccgcc gcgtccctgc cgcccaccgc gctctatcgc gtcggcgtcg gcgacatact
     6121 cgatatacgc ctcgcgggcg gcatgtcgcg cgattcgacg ctcttcacgg tgatgtccac
     6181 ggggatgctc gattatccgc tcgcgggcga gccgatcaac gtcgtcggca tgacgaccga
     6241 cgagatcggc gcgcgcctgt cagccgaact caaacgtcgc gccgtctatg accgcgccca
     6301 gtttcgcatc aacgtgcgcg agtacgcgag ccacacggcg accgtgagcg ggttggtgga
     6361 tcagccgggc gcgaaagttc tgcggcgtga agctgtgccg ctctacgtcg tgttgtcgga
     6421 agcgaacccg cgcgcagagg cgggtcgcgc cgtcatcatc tcgcgcgcga cggggcgcag
     6481 caaaaccctc gatctcgcag actccgccgc cctgaacgaa ctgatctcgc atggcgacat
     6541 catcaacatc accacgcgac cgcaggagtt cttctacatc ggcgggcaga ttaacgcgcc
     6601 ggggcagaaa gattttcatc ccggcatgac gctgacgcag gcgttgctcg cttcgggcgg
     6661 cgtggcgcgc gacgcgggca ataaagttaa agtgacggtt tcgcggcaag gcgcggacgg
     6721 gcgtttggta gcgacggaat atctcttgaa cgacatcgag acgggacaga ttcccgatcc
     6781 gcgtttgcag gccggtgatc gcgtcgaagt cggtcgccgc cgctagtcgc cccggcgcgc
     6841 catgcgttaa gttgaaattt aggttcttgt ccggtgcgtg tgaaggttgt atagtggcgc
     6901 acgccggaaa atgatactca aacaacttca gtgctctcgt ctgcctttaa tcgccggacg
     6961 agttttatta actacactag gaacaatcga aaaacttccc tagtccgttc ctgccggagg
     7021 attactggtt tcatgatgca gcataaactc agcgcgttcg cgcttgccgc tgcgcttctg
     7081 tgcgcgcctt tcacgtctgt cctggccgct gcccccgccg ctgccgctcc tctcgcgtcg
     7141 cgcgcggctg tcggcgcaca acaagacgac accccccccg atgtcgagca ggcgatgttc
     7201 accaaggggc agaactttta caatcaaggc cgcttcgaac aggccgcgac gacctttcgc
     7261 gattttctca agaactaccc gaacagcatc atcacggatt taacgctgct ctggctcggg
     7321 cgcacctaca tcgcgcaaag tcgcctgtcg gatgccgagt cggtcgtcgc gcgcctgcgc
     7381 acgatcaagg acacgccgtt cgtggagatc tacgaaggcg aactgcaagc cgcacgcgcg
     7441 gagatggcct cgcgcggcgt gaccgcgcag ccagcggcaa ctccgtccgc gacgaccgag
     7501 cgtgcgcaag ccacttcgaa ccgcccggca gtccgtgttc cggctgcgac gccgacgcca
     7561 acgcccgtcg cgcgtctggt cggcaggtct tctgcgccgc gcacgcgccc gacgcctttg
     7621 cccacgcctt tgcccacgcc ttcggcaact ccgcaaatag cgcgcgccac tcctacgccc
     7681 aaccggacgc cgaccctgcg gatcgagaat acccccaacg ccaacacgca aacgaacgcg
     7741 cccgtcgtgc gtcccagccg tcgcaacacg cgtgcttcgc gtcgcgccca accgcaaaga
     7801 gccaacaccg acacacaggt ggcgaccaac acgcccgcag tgctccccag acccacgccg
     7861 acgcccattc gcccgcgcca gcaggcagca gccgtcgcca cgccgacgcc aattcaactc
     7921 gcgcgcgccg agccgaacga cagcgtgacg agcgcgccgc ccatcgtcaa cagcgcaccg
     7981 tcggggcagg agggcttcgt cttaacggtc aagcaagtgc cgaatctcaa tctggctttg
     8041 agtcgcgccg cgctcgccgc ctcgcccggt caggtcgtgc aactgccgct caccgtaacc
     8101 aacacgggca acaaggaaga tcagtttcgt ctggggacgg atttgcccgc cgagtatcaa
     8161 ccgacgttca gcctcgcttc gggcgggcag gatacgggac tcccgatcct cgtcacgccg
     8221 caactggcga gaggccagaa tctcgaagtg atgctcaacc tgcgcatccc cgaaaccgcg
     8281 ctcgacgggc agcaacgccc cttcatcgtg agcgcggcct cgcagtctga ttatcaaatt
     8341 caacgcgtga ccaatgccgc gctcaccgtc gtcgccgcct cgctggcagg ctcttcgggt
     8401 gtcacgcgcg agagcgtcca gcccggcgaa accttcacgc agacgatctc cgtgcgtaac
     8461 acgggcagcg cgcccgcgcg cagcgcacgc gccgatttcg tcttcagcac agacttcgaa
     8521 ctcgtcaccg ccagcccctt gccgctctac tacgacaacc cctcgcgcac ggcgatctgg
     8581 agcctcggcg atctcgactc gcgcgccaca cgcgagatca ccgtcacgct gcgcgccgtg
     8641 ccgaacgcgc tcgccgccac cgcccccatc gggcgcggga tgctgcgcac cgcttccttg
     8701 ccgacaggct caaactacga cgggccgagc atcgccatcg gacgcgtgcc gcgtgcgcgc
     8761 atcgatcccg tctcgcccgg actcaccgtg acgcccggcg atgtcatctt cattcctttc
     8821 gtcgtgcgca atccgagcaa ctacgccgaa gcctacggcc tgcgcatcac cgcgccgggc
     8881 gcgccctcgg gcacgctcta cgcagacagc aacggcgacg gccagcatca ggactccgag
     8941 ccgagcgtga cgcagacggg acagatcgat ccgcgcggcg gtcaataccc gctgctcatg
     9001 cgcgtggaga ttccgcgttc gaccgccgac cgccagcagt atgcttacaa cgtcgtggca
     9061 agttcgctca gttcgaaccg cgcggcgagc gaagcgagca cggtgttgac ggtggccgcg
     9121 ccgcgcgtcc gcgtccgcac cgaacaggtg accgatacga ccgcgccggg cgacatgatc
     9181 ttctatcgcc tcgtgctcat caacgaaggc gcgggattgg cgaagaacat cgtcgtcaac
     9241 gaagtgctgc ctgaggcgtt gcagttcgtc aactccaacc cgtcgctgaa ctcgcaggac
     9301 gcgcccggcg actcgcgacg cgtcacctgg cgcgtgcccg aactcgcgcc gaacgacacg
     9361 gcggtcttgc tcatcaccgt ccgcctgcgc ccggacctcg ccgccgacgg aacgatcacg
     9421 acgagccaca cgtgggtcta tcaggatacg aacggcaaca cgtatcagaa tcagtgatgc
     9481 ccgctcgccg tctgcgcgcg gcgagcggta aaattggaag gcgaaagcgt cagccgtgaa
     9541 tcgtgaatag attgaagaat tttgtgcgag gctattcacc gttcacgact gacgcttttg
     9601 tttctgctcc cgccacgcaa gcccctgcga caacgcccgc cacgcgtctc acgcgccttt
     9661 cgcgtgcgct catcgccaag tcaatcgtcg aggccttgtt cgtcgtcgcg ctcgccgtct
     9721 atttctccta caccaacatc aatccgttct ttcgcggctc aatcgacgtg gccgacgcgc
     9781 gcaccatcga aggctgggtt gtcaacgaag ccgcgccgga tgaacgcgtc gaagtgcatc
     9841 tctacataga cgaacagttc gtcagccgcc ggagcgcaga cgcgccgcgc gcggacgtgt
     9901 tggccgcagg ccgcagccgc gacgcctatc acggcttcat cttcgccacg ccgccgctcc
     9961 cgccgggtcg catctatcat gcgcgcgtct acgctatgca catcgcgggc gacggcgcgc
    10021 tgcgcgtgct gcaagagatc ggtgcggcga ccgcattcag tgtgacggcg gacgaagaga
    10081 gcgtccacgt gcctgatgac tggcgggaaa gtgagggg
//
LOCUS       DASWNS010000164        20814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_33059, whole genome shotgun sequence.
ACCESSION   DASWNS010000164 DASWNS010000000
VERSION     DASWNS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20814
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33059"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VGB76_05695"
     CDS             <1..915
                     /locus_tag="VGB76_05695"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY0098429.1"
                     /translation="GRIFNDRGQADFPRGFVRSQDPHGYIFQFWAGRRNERAPTVVPN
                     NPPTTAVSASPSRVVLAAECPPDQRPVEGCTATGSTVQLAANATDPDGDTLLYTWSTT
                     GGRITGDGANVTWDLSGVQPGTYTASVEVDDGCGCIAFSSTTVTVERCTCEVIPIPPT
                     PPPTPTPEVVVPEVRPTPSQFDTYGRIARNDEKARLDNFAVALQNDPGAQGYIIAYGG
                     RRGPAGEAQTRADFARNYLVTARQIDAGRLITVDGGFREEATTELWLVPSGATPPTAS
                     PNVDASEVQTTRPPRRAPRRRGRRDDDE"
     gene            1223..3631
                     /locus_tag="VGB76_05700"
     CDS             1223..3631
                     /locus_tag="VGB76_05700"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY0098430.1"
                     /translation="MKKISLYFVLLTLTASLVLPPRATVAATTTHFIDTTQDAADRTL
                     SWSIAGPMGGDVRELVIDPSNPQHLYLGTIDGQIYQSTDGARTWSRLANFNRSGIYID
                     HIIIDPRDPKTLYVAAHKHKEPGGFFKSTDGGETWRESVELKTEALHSLAQSPSDPNI
                     LIVGSNRGVYRSSDSGETWEQLPTSAYPDIRNIESIAVDPRDPKAIYVGTWHLPWKTV
                     DGGQTWKSIKTGMIDDSDVFAIEIDKTRPDHVIASACSGIYESHNAGANWRKVQGIPS
                     QSRRTRDILQHPAIPSTIFAGTTEGFWRSTNGGNSWMLNTSRTLEINSIAVHENDPQT
                     IYIGTNNYGVMVSRDSGKNFLPSNEGYSGRRAYAIMPDHERPGRVYASTINTATGGGF
                     FFVSNDGGTTWQPSMRNMPSRLIGYSILQDRTDGNIIYLGTNLGMYRSTDRGLSWSPI
                     GAPNEASPAAKPKKKPASTAASRRKKATATSAAAETRPAPPVPVPAKRADDMVKRAQA
                     ALNKAGYNVGTPDGQAGTRTVAAIRQFQAAKELPQTGKFDDATLTALGLGGGKQSIGV
                     EQGIQSAPIALTETVNALAYLQEADGSLKILAATNNGLYRSVDPVKGWEKLPFGPNLD
                     ARTLCVSTSPQNPQTIYVGTSTSGVLVSRDSGQTWREVEGIPTTVPINVIEQDPQRSA
                     YVYVGTSHTLFVSHDGGEKWKRRGGNLPYGSFTSILINPKNTDEIFVGSAYERAEGNG
                     VFHSMDAGQTWKRVDPELPSRRVWALAFDTRDSGKLLIGSHSAGVYVAQRDQSAAALE
                     TKEQ"
     gene            4101..6284
                     /locus_tag="VGB76_05705"
     CDS             4101..6284
                     /locus_tag="VGB76_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY0098431.1"
                     /translation="MSTEDNKLSASEATNENSPTGASDAPEATTAAGEQPNASSSTAA
                     TAATTAPVATTSSSPPAASKAASSAQDDDDDDEQRGGGDFDFGAMLDQYEQEQAAFQE
                     GSVVRGTVVGINDRGVLIDFGFKSEGLVDQQEFTENGELTVKRGDEVEVLIKSMESQE
                     GQPILSRADAVRMRAWDELEKAHRDGTPIKGRIIERVKGGLRVDVDGVDAFLPGSQVD
                     SRPVRNLESFRGQEIEAKVIKLNRKRSNVVLSRKAILDEQNEGRKGETLDNLEESIIV
                     DGQIKNLTDYGAFVDLGGIDGLLHVTDMSWGRLQNPAELFRVGETVQVKVLKFDRERE
                     RVSLGYKQLQPDPWESVEERFIIGSKVQGKVASVTDYGAFVELEAGVEGLVHVSEMSW
                     SKRMKHPSKLVNVGDTVEAEVLGVDPKARRISLGMKQMQANPWQTLRERYQIGARVSG
                     RVRNLTDFGAFIEVEDGVDGLVHVSDISWNKRIKHPGEVLKKGQEIEAVITNIDTENR
                     RLSLSIKDTEPSSWDRFVNEHKPGDIVRGRITRFANFGAFVELDEGLEGLCHISELSE
                     ERVDKPEDVAQVGQEMDFRVLRIENDNKKIGLSARAASSDEPVVDTKSYSTEAKGGMA
                     SLAELGNFFAASSGGATSSRESGSTASGESREAASGGAGSGGANASQEASGEATATDT
                     ASATGGGGAAASDAGEATATLSGGDADASTGDGSDAAGTTEENRS"
     gene            6635..6916
                     /locus_tag="VGB76_05710"
                     /pseudo
     CDS             6635..6916
                     /locus_tag="VGB76_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            7145..7654
                     /locus_tag="VGB76_05715"
     CDS             7145..7654
                     /locus_tag="VGB76_05715"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY0098432.1"
                     /translation="MDRLWTPWRYQYISGAKAENSGDAPSPSDSCIFCTLSQADPSQD
                     ERNFILHRARHNFIVLNLYPYTSGHLLVIPYQHSSELDSISKEASDELMDLAKRAQTL
                     LRAAYQPDGFNLGMNLGSAAGAGVAGHVHLHILPRWTGDANFMTTAGETRVLPEDLPT
                     TYQKLLGKF"
     gene            complement(7711..9591)
                     /locus_tag="VGB76_05720"
     CDS             complement(7711..9591)
                     /locus_tag="VGB76_05720"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEY0098433.1"
                     /translation="MSSRRRPKFTNRYTDARRAAVRRLVSLRDWFRPTTLFIVGFTFL
                     SLVTTLLLAQIHTQLPAQMYSEGDIVRASVVSPADITAVDERATQAARAVSPDAPPVL
                     IALKRNQVVARSGDTVTTKMLAQFDAISKYSDTGRRPQHFIGLLLIVAALYLGAWKFI
                     EYHAQTSTLSLSKERAFTLVASAILIQVILMRTGAILAETIAAQSVVAPWNDATLWSL
                     AIPFAVATLLVALLMGGPLALTVATITALFAGLVSPNGMLMACYAMVSSSAAIYGIGR
                     YSERQSVTLAGLFVAGINSAAALALILAAQEPLTLGTILMAVGCGLLGGLFTTIFTAG
                     ALPVSESLFGILTDVKLLELSNADIPVLGQLALRAPGTNQHSHAVGQLTEEACRSIGA
                     NALLARIGALYHDIGKLAAPDMFVENQTGTNPHDRLRPSSSAKIITSHVTYGQKLAKE
                     IGLPAKIAAFIPQHHGTRTLHYFLRKAQAQAIHGDQVNEAEFRYPGPRPQFKESAIMM
                     LADSCEAAARSLARPDPENIRSIVSKIFDAIVSDGQLDECDLSLRELTTIKESMIASL
                     VAIYHSRVDYPGFNPPPLTGALPPLPQPELDTEERGLGYKTSEVPISKGGEVEDEAIT
                     RQ"
     gene            complement(9604..11262)
                     /gene="hflX"
                     /locus_tag="VGB76_05725"
     CDS             complement(9604..11262)
                     /gene="hflX"
                     /locus_tag="VGB76_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY0098434.1"
                     /translation="MTPEFARQLSELSHETGRQIGALIDRKGYIESIIVGDAKRIELP
                     DLRRTRVAADRFRGLRCVHTHLRGEELTQDDLTDLALLRLDLMVVVDVEQRTGLPGLV
                     RAAHLLPTTAAEHEDGNLGDPYVFLDPQIPSQLDVDFLALINSLEDEMARNRRTTRRA
                     EGRDRAILVGVATGSSSDAEESMAELRELAASANLMVLDHIIQRRATIDPRTLLGRGK
                     IDELLVRALRLGADLIIFDRELSPAQVRSLSEATDLKVIDRAQLILDIFAQRAESREG
                     KIQVELAQLKYLLPRLITGQDSAFSRLAGGIGGRGPGETKLETDRRRVRDRINRLDKE
                     IDTLRQRRQQRRKERTRREIPIISIVGYTNAGKSTLLNTLTSSTVHAEQRMFATLDPT
                     SRRLRLPYEQEVILNDTVGFIRDLPPDLIAAFRATLEEIADSDLLIHLIDASNPRWEQ
                     QLESVDKILADLDFGNIPRLVVFNKSDLVDAETLEGIVRQSCASVSSRQCIAVSANDA
                     RTLRPMLEMAGAILARDLTSDQPASASHARKHAPHAEGVAHNTK"
     gene            11886..12917
                     /locus_tag="VGB76_05730"
     CDS             11886..12917
                     /locus_tag="VGB76_05730"
                     /inference="COORDINATES: protein motif:HMM:NF040432.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098435.1"
                     /translation="MGEAGTITRIDPQAAIPGGEIAIDCEDYDTSNLRACRADFDGER
                     GWLVGASPRRVLAIVPETDWGGEVNVSLKSGDERQSAPARVQVGRKLADDLHMVASPA
                     FDPEDGSLYVTRSGTRGQREPVSLFHIDTSGVIHDVTGDITNPTGLAFDSSGQLFVTS
                     RNDEIVYRIAQFNEAVPFARNLGVATGLAFDRQGRMYVGDRTGTIYRVNAIGEVQPWA
                     QLEPSVAAYHLAFGPDDALYVTGPTVSSYDAVMRVDTSGKADIFFRGLGRPGGLAFDT
                     EGFLYVAASYRARRGIVRISPDAMEARLVVAGMNVIGLAFSAQGEMVVATNEAVYSLP
                     LGIHGTLLK"
     gene            13074..13766
                     /locus_tag="VGB76_05735"
     CDS             13074..13766
                     /locus_tag="VGB76_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0098436.1"
                     /translation="MDEARIGQFILYMVALIFSLSVHEAMHAWMSNRFGDDLAHSLGR
                     VSLSPVTHVDPIGTLLFPAIAFFTGAPLLGWARPTPVNPLRWREKRKANFWVSIAGIL
                     GNLTIAVVVGIIILVLMNMGLVFALTDGRFYALRAANLESTMQGVSALLETFFVMNVG
                     LAVFNLFPIPPLDGSKILQSILPESFGSAIESIEQYGFIILLLALVTGAFRAVFSVVI
                     PLAWKILFIGMG"
     gene            13773..14768
                     /gene="trpS"
                     /locus_tag="VGB76_05740"
     CDS             13773..14768
                     /gene="trpS"
                     /locus_tag="VGB76_05740"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY0098437.1"
                     /translation="MKRIFSGAQPTGNIHLGNYLGAVRNWVELQHEYESLFCIVNLHA
                     ITLPQDPKLLAQKTLDLARFYLAAGIDPKVSLVFIQSDVPEHAELAWILNCVTRISEL
                     ERMTQFKDKAGKQRESVGAGLLNYPVLMASDILLYQTDLVPVGHDQKQHLELTRDLAI
                     RFNRDHGETFRVPEPFIPKVGAKIMSLSDATRKMSKSDENINGSITLLDDADTVRRKF
                     KRAVTDSGTEITFDEKRPAITNLLTIYQILTGKQPAEVEANFAGKGYAALKSELADVT
                     IEFLRPLQERIQGYSDAELERLLDEGRERAQGIARATLDDVKARLGLVGAQGRGA"
     gene            14772..15773
                     /locus_tag="VGB76_05745"
     CDS             14772..15773
                     /locus_tag="VGB76_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEY0098438.1"
                     /translation="MAEMNELKDQAAEPGAHATSGTNSSGAVEADAPDASERDSHDGD
                     PAGGVAFSDSTGSDDEAAVSSDAAPNGAGDSSENAPSLNVAGARPEIVSEGGEGGEEL
                     KLYLGEFAGPLDLLLYLIRQEQVDIYDIPIARITDEYLRYLRLMQDMDIAVASEFLVM
                     AAQLIEIKSKMLLPPDPVADAAQAEGEDPRNELIKQLLEHQKFKAAAQMLWSRSTVEQ
                     AVYVRAPIETDKANPEVSAGVFDLLKIFQEILARKKQEVLMEIERDEITMTEMLERLR
                     NMILSAGELNLRLFFERAQTRRELILAFLSVLEIVRTAEIVLVQETTFGDIVARVNS"
     gene            15789..16691
                     /gene="scpB"
                     /locus_tag="VGB76_05750"
     CDS             15789..16691
                     /gene="scpB"
                     /locus_tag="VGB76_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY0098439.1"
                     /translation="MSREEEQLEREPYDEQDIAVAASGDEAGNTTGDASSRESQDDAA
                     SHASPDNASLESEDDANDFAVAPGDDEDDEEEEEEVVIHDADAEDAAEKLAEMRREMA
                     GRADTSGVASVDDELAPGGSRSMSELISIIESLIFVSEDPLPTKTLADVLKEDRGWVQ
                     VAVEDLAKEFNERNGGLMLREVAGGWQFATRPENHEHVRAFLKSRPSAKLSLAALETL
                     AVIAYKQPITVPEILEIRGVQSSSAIKTLLDKRLIVSKGRKETVGRPMMYGTSKEFLL
                     QFGLRDLSELPNVEDFEDLATGGA"
     gene            16775..17629
                     /locus_tag="VGB76_05755"
     CDS             16775..17629
                     /locus_tag="VGB76_05755"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY0098440.1"
                     /translation="MEERLQKLIAAAGLASRRHAEEMIAAGEVTVNGKLVTEAGTKAD
                     PARDHIKVRGRLINPLLEAREKVYVLLNKPRGYLASLSDPEERPLVTELVPPSLGRLH
                     PVGRLDFNTEGLLILTNDGELTNYITAARNGVAKVYEVKVKGVPPEEAIERLRRGIRL
                     DDGALTAPAQITPTGETGTNAWFEVVLHEGRNQQIRRMFDQIGHSVLKLRRVAIGALR
                     DERLPVGAYRLLHPGEVARLKPRAAKKRGAPATSKGATKTTSNAASKTAAKAASKSRG
                     AGTGRRRS"
     gene            17739..18881
                     /locus_tag="VGB76_05760"
     CDS             17739..18881
                     /locus_tag="VGB76_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0098441.1"
                     /translation="MNFELNDEQRQVKMSVREFAEGEIAPHVREWDEKQHFPVELLPK
                     LAELGLMGVIFPEKYGGAAMGYVEYATIIEELSRVDGSVGISVAAHNSLCSNHIYMFG
                     TEEQKQKYLVPLARGEQLGAWGLTEAGAGSDASGTRTTAVKKNGGWVVNGSKNFITHA
                     IHGDVCVAVASTDRAMRSKGITAFIFEKGMKGFMPGKKEDKLGLRASETASVIFEDCF
                     ISDNQRLGPEGLGFIQAMQILDGGRISIAALAVGIAQGAYESALKYSKERQQFGKPIS
                     EFQAIQFKLSDMATQIDAARLLMYRAAYLKDNGKKVTKESAMAKLYASEISVKVCEEA
                     IQIHGGYGYTKDYPPEKYWRDSKLCTIGEGTSEIQRMVIARQLLNQ"
     gene            18969..19928
                     /gene="meaB"
                     /locus_tag="VGB76_05765"
     CDS             18969..19928
                     /gene="meaB"
                     /locus_tag="VGB76_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY0098442.1"
                     /translation="MITATTKSTELERILAGEARAVARAISKVEDNVGDAAALMKSVF
                     PHTGRGLVVGVTGAPGAGKSSLVDRLAGLYRERGERVGIVCIDPSSPFSGGAILGDRI
                     RMQTLGLDPNVFIRSMATRGHLGGLSRATVDAVAILDAAGFQKIIVETVGVGQDEVEI
                     VKTADVSVVVLVPGMGDDVQALKAGIMEISDVFVINKADRDGVHRTEKELEAMLSLTM
                     RPDLWQPPIVKTVATENKGIDELAAAIASYREYQQQAEASHTRRSSIARWRILELLRE
                     ELVTRAMSHEGADAQLDALATEVAEKRRDPYSAVEEILKAVGK"
     gene            20032..20448
                     /gene="mce"
                     /locus_tag="VGB76_05770"
     CDS             20032..20448
                     /gene="mce"
                     /locus_tag="VGB76_05770"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEY0098443.1"
                     /translation="MKIEHIGIATHKIDDALAFWRDALGVRVLETEEVAEQGVRLAML
                     PVGESRVELLEPINESSPIAKFLEKRGPGIHHIAVRVDDIRATLAHLKEQGVRLIDDA
                     PRTGAGGCLVAFVHPSAASGVLLELVEHVASGHNSE"
     gene            20465..>20814
                     /locus_tag="VGB76_05775"
     CDS             20465..>20814
                     /locus_tag="VGB76_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098444.1"
                     /translation="MNSTYSRAILAFACLLMIPASLWAQKTGRSGRTRSTPPAQTKTA
                     PRTTPATPQQTPQSVNLSAQDLALLIEELGVPPQARAQLASNEASRKEFVQDLREMFA
                     VADEAKAAGFTARP"
BASE COUNT         4308 a         6129 c         6540 g         3837 t
ORIGIN      
        1 ggtcgcatct tcaacgaccg cggccaggcg gacttcccgc gcggcttcgt ccgctcgcaa
       61 gacccgcacg gttatatctt ccagttctgg gccggtcggc gcaacgaacg cgctcccacc
      121 gtggtcccga acaacccgcc gacgacggct gtttcggcaa gcccgtcgag agtggtcttg
      181 gcggctgaat gcccaccgga tcaacgtccg gtcgaaggct gcacggccac gggcagcacg
      241 gttcaactcg cggctaacgc gactgatcct gatggcgaca cgctcctcta cacctggtca
      301 accacgggcg gacgcatcac gggcgacggc gccaatgtga cgtgggattt gtcgggcgtt
      361 cagcccggca cgtacacggc ctccgtggaa gttgacgacg gttgcggttg tatcgcgttc
      421 tcttcgacga cggtgacggt tgaacgttgc acgtgcgaag tgataccgat tccgccgacg
      481 ccgccgccga caccgacgcc tgaggttgtg gtgccggaag ttagaccgac gccgtcgcag
      541 ttcgacacct acggcaggat cgctcgtaac gacgagaaag cacgtctcga caacttcgcc
      601 gtggcgttgc agaacgatcc gggcgcacag ggttacatca tcgcctacgg cggacgccgt
      661 ggaccggctg gtgaagctca gacgcgtgcc gatttcgcca gaaactacct ggtgactgcg
      721 cgtcaaatcg acgccggacg actcattacc gtggatggcg gattcagaga agaggccaca
      781 accgaactct ggctcgtccc gagcggtgcg acaccgccca ctgcgtcgcc aaacgttgac
      841 gcgagcgagg tgcaaaccac acgcccgcca agacgcgcgc cgcgaagacg cggcaggcga
      901 gacgacgacg agtagccgat tgacaaacgc tcgcgcacgc ggcctctcat gagccgcacg
      961 cgacccttag accgaacgcg ggcgatgttt ctcttacaag aagcatcgcc cgtttcgctt
     1021 tttacctgcg cgccgccccg cgacagatgc gcgtacaacg gctccaatcc aaacgcgcat
     1081 cttataagtt atgaaattcc cccgaattct ttatgataaa cgcggcgaag gatagttgat
     1141 cgcctacgtg tcagccgttg aaaacatccc ccctttgagt tttaaatgcc agatgccata
     1201 gaggaaactg atgacccggc caatgaaaaa aatatccctg tacttcgttc ttttaacttt
     1261 gacggcctcg cttgtgttgc cgccgcgtgc gaccgtcgcc gccaccacca cacacttcat
     1321 tgacacgacg caggatgccg ccgaccgcac cctgagttgg tctatcgccg ggccgatggg
     1381 cggcgacgtg cgcgaactcg tcatcgatcc gagcaatccg cagcatctct atctcggcac
     1441 gattgacggc caaatttatc agtctacgga cggcgcgcgc acctggtcgc gcctcgcaaa
     1501 tttcaatcgc tccggcatct atattgacca tatcatcatc gacccgcgcg acccgaagac
     1561 cctctacgtc gccgcgcaca agcacaagga gccgggcggg ttcttcaagt caaccgacgg
     1621 cggcgagaca tggcgtgagt cagtcgaact caagaccgag gcgttgcact cgctcgcgca
     1681 gtcgccttcc gatccgaata tcttgattgt cggttcgaat cgtggcgtct atcgctcaag
     1741 tgattccggc gagacgtggg agcagcttcc gacctcggcc tacccggata tacgcaacat
     1801 cgaatccatc gccgtcgatc cgcgcgaccc gaaagcgatc tacgtcggca cgtggcatct
     1861 gccctggaag actgtggacg gcgggcagac ctggaagtcg atcaagaccg gcatgattga
     1921 cgactcggac gtgttcgcca ttgagattga taagacgcgc cctgatcacg ttatcgcctc
     1981 tgcgtgcagc ggcatctacg agtcgcataa cgcgggcgcg aactggcgca aggtgcaggg
     2041 catcccttcg caatcgcgtc gcacgcgcga catcctgcaa cacccggcca tcccttcgac
     2101 catcttcgca gggacgaccg aaggcttctg gcgttctacc aacggcggca attcgtggat
     2161 gctcaacacc tcgcgcacgc ttgagattaa ttccatcgcc gtgcacgaga acgacccgca
     2221 gacaatttat atcggcacaa acaactacgg cgtgatggtt tcgcgcgata gtggtaaaaa
     2281 tttcctgccg agcaatgaag gctacagtgg acggcgtgcc tacgccatca tgcccgacca
     2341 cgaacgaccg gggcgcgtct atgcctcgac gatcaacacg gcaacgggcg gcggcttctt
     2401 tttcgtcagc aacgacggcg gcacaacgtg gcagccttcg atgcgcaaca tgccgagccg
     2461 cttgatcggt tattccatcc tgcaagaccg caccgatggc aacatcatct atctcggcac
     2521 aaacctcggc atgtatcgtt caactgaccg gggcttgtcc tggtcgccaa tcggcgcgcc
     2581 gaacgaggca tcgcccgccg ccaagccgaa gaaaaaaccg gcctcaaccg ctgcatcgcg
     2641 tcgcaaaaaa gctaccgcca ccagtgcggc agcagagacg cgccctgcgc cgcccgtccc
     2701 cgtcccggcc aaacgcgccg acgatatggt taagcgtgcg caggccgctt tgaacaaggc
     2761 gggttacaac gtcggcacgc ccgacgggca ggccggaacg cgcaccgtcg ccgccatcag
     2821 gcaatttcaa gcggcaaagg aactgccgca gacaggcaag ttcgacgatg cgactttaac
     2881 cgccctcgga ctcggcggcg gcaaacaatc aatcggcgtc gaacagggaa ttcaatccgc
     2941 gcccatcgct ttaaccgaaa ccgtcaacgc gctcgcttat ctacaggaag ccgacggcag
     3001 tttgaagatt ctcgctgcga ccaataacgg actctatcgc agcgtcgatc cggtgaaggg
     3061 ctgggagaag ctgccctttg gtccaaatct cgatgcgcgc acgctctgcg tctccaccag
     3121 cccgcaaaat ccgcagacca tctacgtcgg cacctcaacc tccggcgtgc tcgtctcgcg
     3181 cgatagcggg cagacgtggc gcgaagtcga aggcatcccg acgaccgtgc cgatcaacgt
     3241 catcgaacaa gacccgcagc gttctgctta cgtctatgtt ggcacgtcgc acacgctttt
     3301 cgtttcgcac gacggcggcg agaagtggaa gcggcgcggc ggcaatctgc cttacggcag
     3361 cttcacgagc attctcatca acccgaaaaa cacagatgag attttcgtcg gcagcgcata
     3421 tgaacgagcg gaaggcaacg gcgtctttca ttcgatggat gccgggcaaa cctggaaacg
     3481 cgtcgatccc gaactgccca gccgtcgcgt gtgggcgctc gccttcgaca cgcgcgactc
     3541 aggcaaactg ctcatcggtt cacactcggc gggcgtctat gtggcgcagc gcgaccagag
     3601 cgcggcagca ctggaaacta aagagcagtg aagagcgaca ggtgacgggt gacaaaaaaa
     3661 cgaaagccgt aaatcgtgaa tcgttgatcc tgaatagaag caaacaggtc tttctattca
     3721 cgattcacga tttacggctt ttcttgtcac gcgtcactcg tcaatgcccg tatcgccttg
     3781 acatgcttgc gcgtagacgt gtacattttc gctctacccg gtcaagcgac gggtgattac
     3841 cggatcacgc gggtgatcca cgggcacgac ggaaaccgaa acctttttta aagccttttc
     3901 aacttctaga cggctcgcca tccccgcctc gtcccctctg gatcatccgg caatcacccc
     3961 cacatcgcca gcagagtagg ccgcgcaacc caacgtctac ggtgtggttg agccgcgagc
     4021 gtgagtagtc gctgtccgaa cggggaggcg cgaaagttct ttaacgcgcc gcgaattcca
     4081 gtaggagtcc aagagtccgc atgtccactg aagataacaa gctgagcgca agcgaagcta
     4141 ccaacgaaaa ctcaccgacc ggcgcgagcg acgctcccga agccaccacg gcggcgggcg
     4201 agcagccaaa cgcctcctca tcgacggcag ccaccgcagc caccaccgca cccgtcgcca
     4261 ccacttcatc ttcaccgccc gctgccagca aagctgcgtc gagcgcgcag gacgatgatg
     4321 acgacgacga acagaggggc ggcggcgatt tcgatttcgg cgcgatgctc gatcaatacg
     4381 agcaggagca agcggctttt caggaaggct cggttgtgcg cggcacggtg gtcggcatca
     4441 atgatcgcgg cgtgttgatc gatttcggct tcaagtccga aggactcgtc gatcagcagg
     4501 agttcacgga gaacggcgaa ctgacggtca agcgcggcga cgaagtggaa gtgctcatca
     4561 agagcatgga gtcgcaggaa gggcagccga tcctttcgcg cgccgacgcc gtgcggatgc
     4621 gcgcgtggga tgaactggag aaagcccacc gcgacggtac gccgatcaaa gggcgcatca
     4681 tcgagcgcgt gaagggcggc ctgcgcgtgg acgttgacgg cgtggacgca tttttgcccg
     4741 gctcgcaggt tgattcgcgc cccgtccgca atctcgaaag ttttcgcggg caggagatcg
     4801 aagcgaaggt catcaagctc aatcgcaagc gttcgaacgt ggtgctttcg cgcaaggcca
     4861 tactcgatga gcagaacgaa gggcgcaagg gcgaaacgct cgataacctc gaagagagca
     4921 tcattgttga cggccagatt aagaacttga ccgactacgg tgcgtttgtc gatctcggcg
     4981 gcatcgatgg tctgctccac gtcacggaca tgtcctgggg acgcctccag aatccggcgg
     5041 agcttttccg cgtcggcgaa accgtgcagg tcaaggtctt gaaattcgac cgcgagcgcg
     5101 agcgtgtctc gctcggttac aaacagcttc aacccgaccc gtgggaatcg gtcgaagagc
     5161 gtttcattat cggcagcaag gtgcagggca aggtcgcgag cgtgacggac tacggcgcgt
     5221 ttgtcgaact cgaagcgggc gtcgaaggct tagttcacgt ctccgagatg agctggtcga
     5281 agcgtatgaa gcacccttcg aaactcgtca acgtgggcga tacggtggaa gcggaagtgc
     5341 tcggcgttga cccgaaagcg cgtcgcatca gcctcggcat gaagcagatg caggcgaacc
     5401 cctggcagac cctgcgcgag cgttaccaga tcggcgcgcg cgtctccggg cgcgtacgca
     5461 acctgacgga cttcggcgcg ttcatcgaag ttgaagacgg cgttgacggt cttgtccacg
     5521 tctccgatat ttcgtggaac aagcgcatca agcatccggg cgaggtgttg aagaaagggc
     5581 aggagatcga agccgtcatc accaacatcg acacggagaa tcgccgcctc tcgctctcga
     5641 tcaaagacac ggagccgagt tcgtgggatc gcttcgtcaa cgagcacaag ccgggtgaca
     5701 tcgtgcgcgg gcgcatcacg cgcttcgcca acttcggcgc gttcgtcgaa ctggatgaag
     5761 ggcttgaagg tctgtgccac atctccgagt tgtcggagga acgtgtggat aaaccggaag
     5821 acgtagcgca ggtcgggcag gagatggact tccgcgtgct ccgcatcgaa aacgataaca
     5881 aaaagatcgg gctttcggcg cgcgcagcct caagcgacga acccgtcgtt gacacgaaga
     5941 gctattcgac ggaagcgaag ggcggcatgg cctccctcgc cgaactcgga aacttcttcg
     6001 ccgcttcatc cggtggcgca acttccagcc gcgagagcgg ttcgactgca agcggcgaga
     6061 gtcgtgaagc cgcaagcggc ggggctggct ccggcggcgc aaacgcttct caggaagcgt
     6121 ctggcgaagc cacagccacc gatacggctt ccgctaccgg cggcggcggt gctgccgcat
     6181 ctgacgcggg cgaggcgacg gctaccttat caggcggcga cgcggatgcg tcaaccggcg
     6241 acggcagcga tgcggcggga acgacggaag agaatcgcag ctaaacccta actcgattaa
     6301 acttgaagcc ccgcgccttc aaacttgtct tcgctgacga gttgaaggcg cggggtttta
     6361 gtttgtctcg cgcgatcagc gtgagagccg tgcgtagcgt ccgtcgcggg agcgaatttt
     6421 ctgtgcgctt gcggcgacga gtcgccgcca caagcgcgtc cgagcggcct gcccgcgcct
     6481 ttgtcttcgc cctgtaagct attgcctgag agctatttcc gtcttgcgag gaactgacga
     6541 catctgctac tgtacaatgc agttccagag attccggcag ggtcacggca cgtcttcgca
     6601 gttacgccgc gactgcccgt ttgagtggtg aatcgtgaaa ggtaaatgta tgacgaaggc
     6661 tgagttggtc gaagatgtcg cacgtgcggc ggaattgacc aagaaggatg cggagcgtct
     6721 ggtcgagatc gtcttcgaga gcatcatcga gacgctcaat cagggcgaga agatcgaact
     6781 gcgcggcttc ggcagttttc gcgtgcgtga gcgcggcgcg cggcgcggac ggaatcccaa
     6841 gactggcgac ccggttgaca tccccgccaa acgcgtcccc tatttcaagc ccggcaaaga
     6901 gatgaaagag ttgatcaacg aagagactcc cgccgcgtct acggacggtg gctcctcttc
     6961 cgaaacgact tccgcgccga cggaagcgaa cgacgacaac ctcaccgcga cgacgcccgc
     7021 cagcaccggg cgagacgacg gcgacagcag cagcggcagc aacgcgggca acaccggcgg
     7081 tggcagtagc gagatgcctc cggttcattg atcgcccgac ggcgcaagga aaacggcgaa
     7141 cgacgtggac aggttatgga cgccctggcg ctatcaatac atctccggcg cgaaggccga
     7201 aaatagcggc gacgcccctt ctccctcaga ctcctgcatc ttctgtacgc tctctcaggc
     7261 agacccgtct caggacgaac gcaacttcat tctccaccgc gcccggcaca atttcatcgt
     7321 cctcaatctc tatccctaca cttccggcca tctcctcgtc atcccttacc agcactcttc
     7381 cgaactcgat tcaatctcga aagaggcgag cgacgagttg atggatttag cgaagcgggc
     7441 gcagaccctc ttgcgcgcgg cctatcaacc tgacggcttc aatctcggca tgaatctggg
     7501 gagcgcggcg ggcgcaggcg tcgccggaca cgtccaccta cacatcctgc cccgctggac
     7561 aggcgatgcg aatttcatga ccactgccgg agaaacacgc gtccttcccg aagacctccc
     7621 gaccacttac cagaaactac tcggcaaatt ctaaaaacag tgacgagtga cggcttttgt
     7681 tttaactcgt cacttgtcac ccgtcacctg tcactgtctt gtaatcgctt cgtcttcgac
     7741 ttcgccgcct ttgctgatgg ggacttcgga agtcttgtag ccgaggccgc gttcttccgt
     7801 gtcgagttcg ggttgcggga ggggcgggag cgcgcctgtg agcggcgggg ggttgaagcc
     7861 gggatagtct acgcgcgagt ggtagatggc gacgagcgag gcgatcatgg actctttgat
     7921 cgtcgtcagt tcgcggagcg atagatcgca ttcgtcgagt tgaccgtcgc tgacgatggc
     7981 gtcgaaaatt ttgctgacga tgctgcggat gttctccggg tcgggacgcg ccagcgaacg
     8041 cgccgccgct tcgcacgaat cggccagcat catgatcgca gattccttga actgcggcct
     8101 cggacccggg tagcgaaact ctgcttcgtt cacctgatcg ccgtggatgg cctgcgcctg
     8161 cgctttgcgc aaaaagtagt ggagcgtgcg tgtgccgtga tgctgcggga tgaaggctgc
     8221 gatcttggcg ggcaagccga tctcttttgc caatttctgc ccgtaggtaa cgtggctggt
     8281 gatgatcttc gcgctgctcg aagggcgcag gcggtcgtgc gggttcgtgc ccgtctggtt
     8341 ctcgacaaac atgtcgggcg cggcgagttt gccgatatcg tgatagagcg cgccgatgcg
     8401 tgcgagcaga gcgtttgcgc cgatggagcg acaggcttcc tccgtcagtt gcccgacggc
     8461 gtgcgagtgt tggttcgtgc ccggcgcgcg caacgccagt tgaccgagca cggggatgtc
     8521 tgcgttcgac agttcgagca atttcacgtc cgtcaggatg ccgaagagcg actcgcttac
     8581 gggcagcgcg cccgccgtga agatcgtcgt gaacaggccg ccgagcagcc cgcaaccgac
     8641 ggccatcaga atcgtgccga gcgtgagagg ttcctgcgcc gcgaggatga gggcgagcgc
     8701 ggcggcggaa ttgatgcccg ctacgaagag tcctgcgagc gtcaccgatt ggcgttcgct
     8761 ataacgaccg atgccgtaga tcgccgccga cgaagaaacc atcgcatagc aggccatcaa
     8821 catgccgttt ggcgaaacca gaccggcgaa gagtgcggtg atggtggcga cggtcagcgc
     8881 gagcggtccc cccattaaga gcgcgacgag cagcgttgcc accgcgaagg gaatggcgag
     8941 actccagagc gtcgcgtcgt tccacggggc gacgaccgat tgcgcagcga tagtctccgc
     9001 cagaatcgcg cccgtccgca tcagaatcac ttgaatgaga atggccgacg cgacgagtgt
     9061 gaaggcgcgc tccttcgaga gcgagagggt cgaagtttgg gcgtggtact cgatgaactt
     9121 ccacgcgccg agatagagcg cggcgacgat gagcaggagg ccgatgaagt gctgcgggcg
     9181 gcgtcccgtg tcgctatatt tgctgatggc gtcaaactgc gcgagcattt tcgtcgtcac
     9241 cgtatcgccg gaacgcgcga cgacctgatt gcgtttgagc gcgatgagca cgggcggcgc
     9301 gtccggggag acggcgcgcg cggcttgcgt ggcgcgttca tcgacggcgg tgatgtcggc
     9361 gggtgagacg acgctggcgc gcacgatgtc gccttcgctg tacatctgcg cgggcagttg
     9421 cgtgtgaatc tgcgcgagca gcagcgtggt gacgagggaa agaaaggtaa agccgacgat
     9481 gaagagggtc gtcgggcgaa accagtcgcg caggctcacg agtcgccgga cggcggcgcg
     9541 acgcgcatca gtgtaacggt tggtaaactt ggggcggcga cggctactca tcttcttgaa
     9601 aaattacttg gtattgtgcg cgacgccttc ggcgtgcggg gcgtgctttc gcgcgtgcga
     9661 ggcggaagcg ggttggtcgc tcgtgagatc gcgcgcgagg atcgccccgg ccatctcaag
     9721 catcgggcgc agggtgcgcg cgtcgttggc ggagacggcg atgcactggc gcgaactgac
     9781 ggaggcgcac gactggcgca ctatcccttc taatgtttcg gcgtcaacga ggtctgactt
     9841 gttgaagacg acgagacgcg ggatgttgcc gaaatccaga tcggccagaa tcttatcgac
     9901 ggattcgagt tgctgttccc agcgcgggtt cgaggcgtcg atgaggtgga tgagaagatc
     9961 gctgtctgca atctcttcga gcgtggcgcg gaaggctgcg atcaagtcgg gcggcagatc
    10021 gcgtatgaag ccgaccgtgt cgttcaggat cacttcctgt tcgtaaggga gccggaggcg
    10081 gcgcgaagtc gggtcgagcg tagcgaacat gcgctgctcg gcgtggacgg tgctcgacgt
    10141 gagcgtgttc aaaagcgtgg acttcccggc gttggtgtag ccgacgatgg agatgatcgg
    10201 gatttcgcgg cgcgtgcgct ctttgcgtcg ctgctggcgt cgctggcgga gcgtgtcgat
    10261 ctctttgtcg agacggttga tgcggtcgcg cacgcggcgg cggtcagtct ccagtttagt
    10321 ctcgccggga ccacgcccgc cgatgccgcc cgcgagacgc gagaaggcag agtcctgccc
    10381 ggtgatgaga cgcgggagca ggtatttcaa ctgcgcaagc tccacctgaa ttttaccttc
    10441 gcgcgattcg gcgcgttggg cgaagatgtc gaggatgagt tgggcgcggt ctatgacttt
    10501 gagatcggtg gcttcggaga gcgagcgaac ctgcgcgggc gagagttcac ggtcgaagat
    10561 gatgaggtct gcgccaagac gcagggcgcg cacgagcagt tcgtcaatct tgccgcgacc
    10621 gagcagggtg cgcgggtcaa tcgtcgcgcg ccgctggatg atgtggtcga gcaccatcag
    10681 gttggcagag gcggcgagtt cgcgcagttc ggccatagac tcttcggcat cggaactcga
    10741 acccgtggcg acgccgacga ggatggcgcg gtcgcgtccc tctgcgcgac gcgtggtgcg
    10801 gcggttgcgc gccatttcgt cttcgagcga gttgatgaga gcgagaaaat caacgtcgag
    10861 ttgtgaagga atctgcgggt cgagaaagac gtaagggtcg ccgaggtttc cgtcttcgtg
    10921 ttcggcggcg gtggtcggca ggagatgtgc ggcgcgcacg aggccgggca gtcccgtgcg
    10981 ctgctcgaca tccacgacca ccatcagatc aagacgcagc aacgccagat cggtcaagtc
    11041 gtcctgcgtc aactcttcgc cgcgcaggtg cgtgtggacg cagcgcaggc cacgaaaacg
    11101 gtctgcggcg acgcgcgtgc ggcgcaggtc tggcagttcg atgcgcttgg catcgccgac
    11161 gatgatcgac tcgatataac ccttgcggtc gatgagcgcg ccgatctgcc tgccggtttc
    11221 atgcgagagt tcggagagtt ggcgggcaaa ctccggcgtc acgatctgct gcggcgagat
    11281 gcgccgcgag tagagttttt cgatgcgccg aagttgattg ggtttgagtc cctgcgtatt
    11341 gccgtgaacg gtgctgatgt ttgctgcccc cttattattt tattgcccgc gcattccccc
    11401 gtctgcgaca agcgaccgcc gcacgcacca actggcgtga cggttgcggt tgaaagttag
    11461 agagggaggg atgcgagcgg tatgattcgg acgccgtgag aagccttttg atgttgccct
    11521 gcgaaacact tcgcttggtc attttaagcc gcgcgtgcgc tgagttccgt cagccgtgcg
    11581 agataaaaaa acgcgcgctc tttcggttga ctcgctaaat ctaaaagtcg tcaggcgcgg
    11641 attatagcat attgactgcg catgcaagga gcggggcgca caggaaatgc gccgggcgcg
    11701 gcgtggagat gatagccgcg tggacacagt tcgtgcgcgg ggcgtatgat tgacagcatt
    11761 cagccaatta gctattagcc atcagccgtc tgttgcttca agcccggcga gttatcatcg
    11821 ccaaacagac tgttccggct ggcggttgat agctaactgc tgacagcttc tttcggagaa
    11881 acaacatggg tgaggcagga acgattacgc gcatcgaccc gcaggcggcg attcccggcg
    11941 gcgagatagc gattgattgt gaagattacg atacgagcaa cttgcgcgcg tgccgtgccg
    12001 acttcgacgg cgagcgcggc tggttggtcg gcgcatcgcc gcgacgcgtg ctggcgattg
    12061 tgccggaaac agactggggc ggcgaagtta atgtcagctt gaagagtggc gacgaacggc
    12121 agagcgcgcc cgcgcgcgtg caggtcggga gaaaactcgc cgacgatctg cacatggtcg
    12181 ccagcccggc cttcgacccg gaggacggtt cgctctacgt gacgcgctca ggcacgcgcg
    12241 ggcagagaga gcctgtctcg ctttttcaca tcgacacgag cggcgtgatc catgacgtga
    12301 cgggcgacat cacgaaccct accggcctcg ccttcgactc ctcagggcaa ctgttcgtca
    12361 ccagccgcaa cgacgagatc gtctaccgga tcgcgcagtt caacgaagcc gtgcccttcg
    12421 cgcgcaacct cggcgtcgcc accggcctcg ccttcgaccg tcaggggcgc atgtacgtcg
    12481 gcgaccgcac gggcacgatc tatcgcgtca acgccatcgg cgaagtgcag ccgtgggcgc
    12541 aactcgaacc gtccgtggcc gcctaccatc tcgccttcgg acccgacgac gcgttgtacg
    12601 taacgggtcc gacggtttca agctacgacg ccgtgatgcg cgttgacacg agcggcaagg
    12661 cggatatctt ctttcgcggc ctcgggcgac cgggcgggct ggctttcgac acagagggct
    12721 tcctctacgt cgccgcctct tatcgcgccc gtcgcggcat cgtgcgcatc tctcccgacg
    12781 cgatggaggc gcgcctcgtc gtcgccggaa tgaacgtgat cggcctcgcc ttcagcgcgc
    12841 agggcgagat ggtcgtcgcc accaacgaag ccgtctacag cctgccgctc ggcattcacg
    12901 gaacgctctt gaagtgagcg gcgaacggcg agcggtgagc cgcgaaagct gtttcttact
    12961 gcttatcgct catcgctcaa ggctcacttt tccagttttg acacccccgc gaacgcatgt
    13021 tagtgtgacg gttcgtaggc cggattggac acaaatgctt gtgagtttaa gttatggatg
    13081 aagcccgcat cggacaattt attctctaca tggtggcgct catcttttcg ctgtccgtgc
    13141 atgaggcgat gcacgcatgg atgtcgaatc gcttcggcga cgatctggcg cacagtctgg
    13201 ggcgcgtctc tctgagtccg gtcacgcacg tcgatccaat cggcacgctg ctgtttccgg
    13261 ccatcgcttt tttcactggc gcgcccctgc tcggatgggc gcgacccacg cccgtcaacc
    13321 ccttgcgctg gcgcgaaaag cgtaaggcaa acttctgggt gtcgattgcg gggattctgg
    13381 gaaatctcac catcgcggtc gtcgtcggga tcattattct ggtcttgatg aatatggggt
    13441 tggttttcgc gctcacggat ggtcgtttct acgcgttgcg cgcggccaac ctcgaatcca
    13501 cgatgcaggg cgtgtcggcg ttgcttgaga cgttcttcgt gatgaatgtc gggttggcgg
    13561 tcttcaacct gttcccgatc ccgccgctcg acggcagcaa gattttgcag agcatcctgc
    13621 ccgaaagctt cggctcggcc atcgagtcca tcgagcagta cggtttcatc atccttctcc
    13681 ttgcgctcgt gacgggcgcg tttcgcgcgg tattcagcgt cgtcattcct ttagcgtgga
    13741 agattctttt catcggaatg ggataagcca gcatgaaacg aattttcagc ggcgcacagc
    13801 cgaccggcaa cattcatctc ggcaactatc tcggcgcggt gcgcaactgg gtcgaactcc
    13861 agcacgaata cgaaagcctc ttctgcatcg tcaacctcca cgcgatcacg ctgccgcaag
    13921 accccaaact gctcgcgcag aaaacgctcg acctcgcgcg cttctatctc gccgcaggca
    13981 tcgatccgaa agtctccctc gtcttcattc agtcggacgt gccggaacat gcggagttgg
    14041 catggattct taattgcgtc acgcgcatca gcgaactcga acgcatgacg cagttcaaag
    14101 acaaggcggg caagcagcgc gagtcggtcg gcgcgggact cctgaattat cccgtgctca
    14161 tggcctccga tattctgttg taccagacag acctcgtgcc cgtcggccac gatcaaaagc
    14221 agcatctcga actaacgcgc gacctcgcca tccgtttcaa ccgcgaccac ggcgagacgt
    14281 ttcgcgtgcc cgaacctttc atcccgaagg tcggcgcgaa aattatgtcg ctgagcgacg
    14341 cgacgaggaa gatgtcgaag tcggacgaga acataaacgg cagcatcacg ctgctggatg
    14401 atgccgacac cgtgcgacga aagttcaagc gcgccgtcac cgattccggc acggagatca
    14461 ccttcgacga gaagcgtccg gcgatcacga atctactcac gatctatcaa attctgaccg
    14521 gcaaacagcc tgcggaagtc gaagcgaatt tcgcgggcaa gggctacgcc gctttgaagt
    14581 ccgaactcgc cgatgtgacg attgaatttt tgcgcccgct gcaagaacgc atccagggct
    14641 atagcgacgc ggaactcgaa cgcttgctcg atgaagggcg tgagcgagcg cagggcatcg
    14701 cccgcgccac gctcgacgat gtgaaggcgc gtctcggact cgtcggcgcg caggggcggg
    14761 gtgcgtagaa gatggcagag atgaatgagt tgaaagatca ggcagccgaa ccgggcgcgc
    14821 acgctacgtc aggcacgaac tcgtccggcg cggtcgaagc agatgcgcct gacgcatccg
    14881 aacgtgactc gcacgacggc gacccggcgg gcggcgtcgc attctcggat tcaaccggta
    14941 gcgacgacga ggctgcggtt agttctgatg ctgccccgaa tggcgcgggg gattcgtccg
    15001 agaacgcgcc gtctttgaac gtcgcgggcg cgcggcctga gatcgtgtcg gaaggcggcg
    15061 aaggcggcga ggagttgaag ttgtacctcg gcgagtttgc agggccgctc gacctgctgc
    15121 tctacctgat ccggcaggag caggtggaca tctacgacat tcccattgcg cgcatcactg
    15181 acgaatattt gcgctacctg cgcctgatgc aggacatgga catcgccgtc gcgagcgagt
    15241 ttctcgtgat ggccgcgcaa ctcatagaga tcaagtcgaa gatgcttttg ccgcccgacc
    15301 ccgtggccga tgcggcgcag gcggaaggcg aagacccgcg caacgaactc atcaagcaac
    15361 tgctcgaaca tcaaaagttc aaggccgccg cgcagatgct ctggtcgcgc tcgacggttg
    15421 aacaggcggt ttacgttcgc gcgccgattg agacggacaa agcaaacccg gaagtgtctg
    15481 cgggcgtctt cgacctgctc aaaatctttc aggagattct cgcgcgcaag aagcaggaag
    15541 tcctgatgga gatcgagcgc gacgagatca cgatgacgga gatgctcgaa cgcctccgca
    15601 acatgatcct ctcggcgggc gaactcaacc tgcgactttt cttcgagcgc gcgcagacgc
    15661 gccgcgaact catcctcgcc tttctctcgg tgctcgaaat cgtccgcacc gcagagatcg
    15721 tgctcgttca ggagacaact ttcggcgaca tcgtcgcccg cgtaaacagc taaccaagat
    15781 tgaaaacgat gagtagagaa gaagaacaat tggaacgaga gccgtacgac gagcaggata
    15841 ttgccgtcgc tgcgtcaggc gacgaggccg ggaacacgac gggcgacgcg tcgagccgtg
    15901 agtcgcagga cgacgcggcg agccatgcgt cgccggataa cgcgagtctt gagtcggaag
    15961 acgacgcgaa cgactttgcc gtcgcgccgg gcgatgatga ggatgacgaa gaagaagaag
    16021 aagaggtggt gatccacgat gccgacgccg aagatgcggc ggaaaagctg gccgagatgc
    16081 gacgggagat ggccggaagg gctgatacga gcggcgtggc ttccgtcgat gacgaactcg
    16141 ctcccggcgg ttcgcgcagc atgtcggagt tgatctcgat catcgaatca ttgatcttcg
    16201 tctcggaaga cccgctgccg acgaagacgc tggcggacgt gctcaaagag gatcgcggtt
    16261 gggtgcaggt ggcggtcgaa gacctggcga aagagtttaa cgagcggaac ggcggcctga
    16321 tgctgcgcga ggtggcgggc ggctggcagt tcgccacgcg accggaaaat cacgaacacg
    16381 tgcgcgcctt cctcaagtcg cgcccgtctg cgaaactctc gctcgccgcg ctcgaaacgc
    16441 ttgccgtcat cgcgtacaag caaccgatca cggttcccga aattctggag atacgcggcg
    16501 tgcaatcctc ttcggccatc aagacgctgc tcgataaacg tctcatcgtc tccaaagggc
    16561 gcaaggagac ggtcgggcgt ccgatgatgt acggcacgtc gaaagagttt ctcctccagt
    16621 tcggcctgcg cgatctctcc gaactaccga acgtcgaaga ctttgaagat ttagccaccg
    16681 gaggggctta aacacagagg ccggagttta gagatcagtt aaaacatgac tgatttctac
    16741 tcacctccgg cctctcgcct ctgacctcta ctttatggaa gaacgtctgc aaaaattgat
    16801 cgcggcggcg gggctggcct cgcgtcgcca tgcggaagag atgatcgcgg cgggcgaggt
    16861 gacggtcaac ggtaaactcg tgaccgaagc gggcacgaag gccgacccgg cgcgcgatca
    16921 catcaaggtg cgcgggcgtc tgatcaatcc gctgctcgaa gcgcgcgaga aagtttacgt
    16981 gctgctcaac aagccgcgcg ggtatctggc gagtttgtcc gacccggagg aacgcccgct
    17041 cgtaaccgaa ctcgtgcccc cctcgctcgg acggctccac ccggtcgggc gtctggactt
    17101 caacacggaa gggttgctga tcctgacgaa cgacggcgag ttgacgaact acatcacggc
    17161 ggcgcgcaac ggcgtagcga aagtttacga agtgaaggtc aagggcgtcc cgcccgaaga
    17221 ggccatcgaa cgactccggc gcggcatccg cctcgacgac ggcgcgctca ccgcgcccgc
    17281 acagatcacc ccgacggggg agacgggaac gaacgcgtgg ttcgaagtcg tcctgcacga
    17341 aggacgcaat caacagatca ggcgcatgtt tgaccagatc ggccactcgg tcttgaaact
    17401 gcgccgcgtc gccatcggcg cgctcagaga tgaacgcctg cccgtcggcg catatcgcct
    17461 gctccatccc ggcgaagtgg cgcgactgaa gccgagggcg gcgaagaaaa gaggcgcgcc
    17521 cgcgacgagc aaaggggcga caaaaacgac gagtaatgcg gcctctaaaa cggcggcgaa
    17581 agcggcttct aaaagtcgcg gcgcgggtac gggtcggcga cgaagttaag gtacaatgtt
    17641 cggtcagacg aacggcggcg cgagacggat agttgcgtgg taggtctgtc agtgtccgtc
    17701 tttttttgcg cgtactcatc agggggcttt tcaccggcat gaatttcgaa ctaaacgacg
    17761 agcagcgaca ggtcaagatg agcgtgcgcg aatttgccga gggcgagatc gcgccgcacg
    17821 tccgggagtg ggatgagaaa caacatttcc cggtcgagtt gctgccgaaa ctggccgaac
    17881 tcggtttgat gggcgtcatc tttcccgaaa agtacggtgg cgcggcgatg ggctacgtcg
    17941 agtatgcgac gatcatcgaa gagctttcgc gcgttgacgg ttcggtcggc atctccgtgg
    18001 ccgcgcacaa ttcgctctgc tcgaatcaca tctacatgtt cggcacggaa gagcagaaac
    18061 agaaatatct cgtgccgctc gcgcgcggcg agcaactcgg cgcgtgggga ctgacggaag
    18121 cgggcgcggg ttcggacgcc tccggcacgc gcacgactgc cgtgaaaaag aacggcggct
    18181 gggtcgtcaa cgggtcgaag aatttcatca cacacgccat ccacggcgac gtgtgcgtgg
    18241 ccgtcgcctc gaccgaccgc gcgatgcgct cgaagggcat caccgctttc atctttgaga
    18301 aaggcatgaa gggtttcatg cccggcaaga aggaagacaa actgggattg cgcgcgtcgg
    18361 aaacggcgag cgtgatcttt gaagactgtt ttatctcgga caatcaaagg cttggtcccg
    18421 aagggttggg cttcatacag gcgatgcaga ttctcgacgg tgggcgcatc tccatcgccg
    18481 cgctcgccgt cggcatcgca cagggcgcgt atgagtctgc gctgaagtat tccaaagagc
    18541 ggcagcagtt cggcaagccg atctcggagt ttcaagcgat ccagttcaaa ttgtcggaca
    18601 tggcgacgca gatcgatgcc gcgcgcctgt tgatgtatcg cgccgcttac ttgaaggaca
    18661 acggcaaaaa ggtgacgaaa gagtctgcga tggcaaaact ctacgcctcg gagatcagcg
    18721 tcaaagtttg cgaggaagcg attcaaattc acggcggcta cggctacacg aaagactatc
    18781 cgcccgaaaa atactggcgc gattcgaaac tctgcaccat cggcgaaggc acttcggaaa
    18841 ttcaacgcat ggtcatcgcg cggcaactgt taaatcagtg acaagcgacg agtgataagt
    18901 gacaggttaa aagcataaaa cttgtcttta ctcgtcactc gccacttatc actcgtcact
    18961 gttttttgat gatcacagca acgacaaaga gcaccgaact tgaacgcatc ctcgcgggcg
    19021 aagcgagggc ggtggcgcgc gccatctcga aggtggaaga caacgtgggc gacgccgccg
    19081 cgttgatgaa atcggtgttt ccgcacacgg ggcgcggcct cgtcgtcggc gtgacgggcg
    19141 cgccgggagc ggggaagtct tcgctggttg accgtctcgc agggttatac agggagcgcg
    19201 gcgagcgcgt cgggattgtg tgcattgatc cttcaagccc gttttcgggc ggcgcaattc
    19261 tcggcgaccg tatcaggatg cagacgctcg gcctcgaccc gaacgtcttc attcgctcga
    19321 tggcgacgcg cgggcatctc ggcggcctct cgcgtgcgac ggtggacgcc gtagcgatcc
    19381 tcgatgcggc gggtttccag aagatcatcg tggagacggt cggcgtcggg caggatgaag
    19441 tcgagatcgt aaagacggcg gacgtgtcgg tcgtcgtgct cgtgccgggc atgggcgacg
    19501 acgtgcaggc gctcaaggcc ggcatcatgg agatcagcga cgtgttcgtc atcaacaaag
    19561 ctgatcgcga cggcgtgcat cgcacggaga aggaactcga agcgatgctc tcgctcacga
    19621 tgcgccccga tttatggcag ccgcccatcg tgaaaacggt ggcgacggag aacaagggca
    19681 ttgacgaatt ggccgccgcg attgcgagtt accgcgaata ccagcagcag gccgaggcga
    19741 gccacacgcg ccgctcctcg attgcgcgct ggcgcatcct cgaactgctg cgcgaagaac
    19801 tcgtgacgcg agccatgagc cacgaaggcg cagacgcgca actcgacgcg ctcgccaccg
    19861 aagtggccga gaagcgacgc gacccgtatt cggcggtgga agagattctc aaagctgtcg
    19921 gtaaatagcc gccgtttatt agcgttcggc tttcagcttt ttgtgtttag tgaaacccgg
    19981 aagatagatc tccggcaagc agagtcttga gttttaagct aacggctttc tatgaaaatc
    20041 gagcacatcg ggatcgccac tcataaaatt gacgacgcgc tggcgttctg gcgcgatgcg
    20101 ctgggggtgc gcgtgttgga gacggaagag gttgccgaac agggcgtgag actggcgatg
    20161 ttgcctgtgg gcgagtcgcg cgtcgagttg ctggagccga tcaatgagtc gtctcccatc
    20221 gcgaagtttt tggagaagcg aggccccggc attcatcaca tcgccgtgcg cgtggacgac
    20281 atccgcgcga cgctggcgca tctgaaagag cagggcgtgc gcttgattga cgacgcaccg
    20341 cgcacgggcg cgggcggttg ccttgtcgcc ttcgttcacc cgtcggcggc gagcggcgtg
    20401 ttgctcgaac tcgtcgagca cgttgcatcc ggacataatt ctgaataaag ggacgaagag
    20461 aaatttgaat tcgacataca gcagagccat cctcgcgttc gcctgtctcc tgatgattcc
    20521 ggcgagcctc tgggcgcaga aaaccgggcg cagcggtcgc acccgaagca caccaccggc
    20581 gcagaccaag accgcgccgc gcaccacgcc cgccacacca caacagacgc cgcagagcgt
    20641 caacctttcc gcgcaagacc tcgcgctgct catcgaagaa ctgggcgtac cgccgcaagc
    20701 gcgcgcgcag cttgcgagca acgaggcatc gcggaaagag tttgtgcagg acttgcgcga
    20761 gatgttcgcc gttgcggatg aagcgaaggc cgcgggcttt acggcgcggc ctga
//
LOCUS       DASWNS010000165         5549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_33081, whole genome shotgun sequence.
ACCESSION   DASWNS010000165 DASWNS010000000
VERSION     DASWNS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33081"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VGB76_05780"
     CDS             <1..751
                     /locus_tag="VGB76_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEY0098445.1"
                     /translation="WSFARGERAREVALLTDAAPARCGDMLARGAVEAALVPVIEYQR
                     LPDVAVVPGVCVGARRAVRSVVLVTRCDDLTDVRTLALDESSRTSAALVQIIFREFVG
                     REVVTAPASPDVRAMLAAHDAALVIGDPAMTFARENLRVHDLALLWRKFTNLGFVFAM
                     WMAHESAAEAVRRIDFAGARDEGLARLEEIVAEYERELDLPRAELRAYLTENICFDLN
                     ADMRAGLELFFALAHKHKLIQAVRPLKMLDK"
     gene            920..1438
                     /locus_tag="VGB76_05785"
     CDS             920..1438
                     /locus_tag="VGB76_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098446.1"
                     /translation="MRLEALNAPVNGSPAPQTRLSEINLAAIGRIAPKGRVQDREYNQ
                     LPVVEQLLAQGRESVPYLINRLEDETKIEARVLDYWSEVRVGDVALIILTDFFTDKSR
                     QRTTIPGVGWDEFLGGGNDASLTGEDRLRHYIEVRGRKSIKERWQQAWEQHKKNIYWD
                     NAERCFISKSTN"
     gene            1443..2585
                     /gene="mqnC"
                     /locus_tag="VGB76_05790"
     CDS             1443..2585
                     /gene="mqnC"
                     /locus_tag="VGB76_05790"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEY0098447.1"
                     /translation="MTDIQPILDRALAGKRLTAADCTALLESDDIARIGVAADEVRKR
                     KHPSGIVTYIIDRNINYTNVCNVVCTFCAFYRRPGKPDTYVRTVEEICEKIDETIALG
                     GTGILMQGGLHPDFGIEWYEELLRTLHAKYPQVQLHSFSPPEIHNIHLISGLSYEEIL
                     TRLKEAGLSSLPGGGGEILDDEVRKRVSTKCTTDEWLAVMRATHRVGLRSTATMMFGI
                     GDTVAHRVRHLERIRNLQDETGGFTAFIPWTFQRENTALGRRIKDEPTGVDYLKMVAV
                     SRLFLNNIEHIQSSWLTQGLRLGQVVLRFGADDMGSIMIEENVVSAAGAHNEADERML
                     RYLIRTAGFRPQQRDILYNHINRDEATTGDASDSATLRELSVAFAD"
     gene            complement(2729..5041)
                     /locus_tag="VGB76_05795"
     CDS             complement(2729..5041)
                     /locus_tag="VGB76_05795"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY0098448.1"
                     /translation="MPRRSSALTLLFAATLFTSASLLFLVQPMVAKMLLPLLGGTPAV
                     WNTCMLFFQAMLLAGYAYALAVSKWLSFRQQVGAQLLLLLLTALTFPVLISEATVRSV
                     PREGNPSLWLLGSLLTTVGLPFFAVSTFSPLLQKWFAETRQTSSRDPYFLYAASNAGS
                     LLALLSYPLLLEPTLTLRGQSRLWSLAYALLVLLLAACAYALLRRQTKQVETKTPAPL
                     DARDPRDAEINSSIGEALEAHETQLETLTWRRRLRWVALAFVPSSLMLGVTNYFSTDI
                     ASLPLLWVIPLSLYLLTLVFAFAQRQLFKPRALARILPGIALVFALVYLSGATQPVWL
                     LILSHLFFFFVAALACHAQLAADRPSVRHLAEFYLLMSVGGVFGGLFNALLAPVLFNT
                     TLEYPLVIILACLLLPRDRGGVSGRETDAAPTDTPRQRWLDIGVPAAIALLTISLALV
                     VRRFTLDWIQSLALVVGVPLILSYLFRRRPVRFALALGAVMLGSSFHANLERETLIAE
                     RNFFGTLRVTQDDASDTHWLYHGTTIHGRQSHALERHCEPLSYYHREGPLGHVFKTFN
                     EQPANGNVAIVGLGTGATAAYTRAGQDWTFYEINPAVVSIARAPAYFTYLSYCANAPV
                     NVALGDARLQLQNAPAAHYGLIVLDAFSSDAIPMHLMTLEALDLYLSKLAPGGLLVFH
                     ISNRSLDLHPVVADLARARNLSSLVFDDTARNQPSGKEPSQWAVMARRPQDIATLAAD
                     TARWQKLEGHPNRPVWTDDFSNIVSIFKWR"
     gene            complement(5045..>5549)
                     /locus_tag="VGB76_05800"
     CDS             complement(5045..>5549)
                     /locus_tag="VGB76_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098449.1"
                     /translation="SLTLIAPNVSDATNARPDLPGAAFYGLLHSPVLGASFYNAMTSE
                     RSLRDYARKRLFYDERFATPRLVAHYYTASHQPGAQHAVAAFLSGYLNTDTREAFARL
                     TQPVTLVWGKQDLTTPITQAATLLSLNPRARLELFERCRMMPQTEHAEKFNALIRTTL
                     QARSAAA"
BASE COUNT         1112 a         1501 c         1941 g          995 t
ORIGIN      
        1 ctggagtttt gcgcggggcg agcgggcgcg cgaggtggcg ctcttgacgg acgcggctcc
       61 ggcgcgttgc ggcgacatgc tcgcgcgcgg agcggtggag gccgcgctcg tgcctgtcat
      121 cgagtatcaa cgcttgccgg acgtggccgt cgtgccgggc gtgtgcgtgg gcgcgcgccg
      181 cgccgtgcgg agcgtcgtgc tggtcacgcg ttgcgatgat ctgacagacg tgcggacgct
      241 cgcgctcgac gaatcatcgc gcacttccgc cgcgctcgtg cagatcatct tccgcgagtt
      301 tgtgggacga gaggttgtca ccgctccggc ctcgccggac gtgcgggcga tgctcgcggc
      361 gcacgatgcc gcgctcgtga tcggcgatcc ggccatgacc ttcgcgcggg agaatttgcg
      421 cgtgcatgat ctggccttgc tctggcgcaa attcacgaac ctgggtttcg tcttcgcgat
      481 gtggatggcg cacgagtctg cggcggaggc ggtgcgccgg atagatttcg cgggcgcgcg
      541 cgatgagggg ttggcgcgtc tggaagagat cgtcgcggag tatgaacgcg aactcgatct
      601 gccgcgcgcg gaactgcgcg cctacctgac ggagaatatc tgctttgacc tgaacgcaga
      661 catgcgcgcc ggtcttgaac tcttctttgc gctcgcgcac aagcacaaac tcattcaggc
      721 ggtgcgtcct ttgaagatgc tcgacaagta gttcgtgtcc cggacatcag cgcatcgacg
      781 ggcttcaaac aaagcgcggc gcgtggcgta taatgagttg agagagttgc gaaaaatgcg
      841 tggcgtgtct tatatgaaaa gagcctcaat agtctgcatc gcgatgctgt tgtctgtgag
      901 ttgtgggagg gcggacgcta tgaggcttga ggctttgaac gcgcccgtca atggctcacc
      961 cgcgcctcaa acgcggttgt ctgagattaa tctcgccgcc atcggtcgca tcgcgccgaa
     1021 gggtcgagtt caagatcgag aatataatca gttgcccgtg gtagagcagt tgctcgcgca
     1081 gggcagggag tctgttcctt atctcatcaa ccggcttgaa gatgaaacga aaatcgaggc
     1141 gcgcgttctg gattactggt ctgaggtgcg agtgggggat gtggctctaa ttatcctgac
     1201 agactttttt accgataaaa gtcggcagcg cacaactatt cccggcgtcg gctgggatga
     1261 gtttctcggc ggcgggaacg acgcgagcct caccggagaa gatcgcttgc ggcactacat
     1321 tgaggtgcga ggaagaaaaa gcatcaaaga gagatggcag caggcctggg agcagcacaa
     1381 gaaaaatatc tactgggata acgcggaaag gtgtttcatc tctaaaagca caaattgaag
     1441 ttatgaccga cattcaacca attctcgacc gcgcgctcgc aggtaagcgt ttgaccgccg
     1501 ccgattgcac cgccctgctt gaatccgacg acatcgcacg catcggagtg gccgccgatg
     1561 aagtgcgtaa gcgcaagcac ccgtccggca tcgtcactta catcattgat cgcaacatca
     1621 actacacgaa tgtctgcaac gtcgtctgca ccttctgcgc cttctatcgc cgaccgggca
     1681 agccggacac gtacgtccgc accgtcgaag agatttgcga gaagattgac gaaaccatcg
     1741 cgctcggcgg tacgggcatc ctgatgcagg gcggcctcca cccggacttc ggcattgagt
     1801 ggtacgaaga actcctccgc acgcttcatg cgaagtatcc gcaggtgcag ttgcacagtt
     1861 tttcgccgcc ggagattcac aacatacatc tcatcagcgg cctctcttac gaagagattt
     1921 tgacgcgttt gaaagaggcc gggctgtcgt ctctgcccgg cggcggcggc gagattctcg
     1981 acgacgaagt gcgcaagcgt gtctcgacca agtgcacgac cgacgaatgg ctcgcagtga
     2041 tgcgcgccac acaccgcgtc ggcctgcgct cgacggcgac catgatgttc ggcatcggcg
     2101 acacggtggc gcaccgcgtc cgccacctcg aacgcatacg caacctgcaa gacgagacgg
     2161 gcggcttcac ggccttcatc ccctggacgt tccaacgcga gaacaccgcg ctcggacgac
     2221 gcatcaagga cgagccgacg ggcgttgatt acctgaagat ggtcgccgtc tcgcgcctct
     2281 tcctcaacaa catcgaacac atccagtcgt cgtggttgac gcaggggtta aggctcggtc
     2341 aagtcgtcct gcgcttcggc gcagacgaca tgggttcgat catgatcgaa gagaacgtcg
     2401 tctccgccgc cggagcgcac aacgaagcgg acgaacgcat gctccgctac ctcatacgca
     2461 ccgcaggctt ccgcccgcaa caacgcgaca tcctctacaa ccacatcaac cgcgacgaag
     2521 cgacaacggg cgatgcgagc gattccgcca cgctacgcga gttgagcgtc gccttcgccg
     2581 attgaacaga tacattcaga actgctaaca aaacttaacc aggcaagaca taaagcacga
     2641 aacggcacga agatgatttc gtgccgtttc gtgttctttc gtggttgtca gggtttgtta
     2701 gtcgctgtaa atgatcgcgg caagcgcatt aacgccactt gaagatggag acgatgttgg
     2761 agaagtcgtc tgtccagacg gggcggttgg ggtggccttc gagtttttgc cagcgggcgg
     2821 tgtcggcggc gagtgtcgct atgtcttgcg ggcggcgcgc catgaccgcc cattgggagg
     2881 gttctttgcc gctcggttgg ttgcgcgcgg tgtcgtcgaa aacgaggctg gagaggttgc
     2941 gcgctcgcgc caggtctgcg acgaccgggt ggagatcgag gctgcggttg gagatgtgaa
     3001 agacgaggag gccgccgggc gcgagtttgg agagatagag gtcgagggct tcgagggtca
     3061 tcaggtgcat ggggatggcg tcggaactga aggcgtcgag gacgatgagg ccgtagtgcg
     3121 cagccggagc gttttggagt tggaggcgcg cgtcgccgag ggcgacgttg acgggcgcgt
     3181 tggcgcagta ggaaagatat gtgaagtaag ccggggcgcg cgcgatgctg acgaccgccg
     3241 gattgatctc gtagaaagtc cagtcttgac cggcgcgcgt gtaagcggcg gtcgcgcccg
     3301 tgccgaggcc gacgatggct acattgccgt tggcgggttg ttcgttgaaa gtcttgaaga
     3361 cgtggccgag cggtccttcg cggtggtagt aggagagcgg ttcgcaatgg cgttcaaggg
     3421 cgtgcgattg tcgcccgtgg atggtggtgc cgtgatagag ccagtgggtg tcgctggcgt
     3481 cgtcctgcgt cacgcgcagc gtgccgaaga agttgcgttc ggcgatgagc gtttcgcgtt
     3541 cgaggttcgc gtgaaaactg ctgccgagca tgaccgcacc aagcgcgagc gcgaaacgca
     3601 cggggcgacg acgaaagaga taactgagga tgagcggcac gcccacgacg agcgcgagac
     3661 tctgtatcca gtcgagcgta aaacgccgga cgacgagcgc gagcgagatc gttagaagcg
     3721 cgatggccgc agggacgcct atatcgagcc aacgctggcg gggcgtgtct gtaggggcgg
     3781 cgtcagtctc tctgccgcta acgccgcccc ggtcgcgcgg gagcagcagg caggcgagaa
     3841 tgatgacgag cgggtattcg agcgtcgtgt tgaagagcac gggcgcgagg agcgcgttga
     3901 agagtccgcc gaacacgccg ccgacggaca tcagcaggta gaactccgcc agatgccgga
     3961 cggagggacg atctgcggcc aactgcgcgt ggcacgcgag cgcggcgacg aagaagaaga
     4021 agagatgcga gaggatgagc agccagacgg gctgcgtcgc gccggagaga tagacgaggg
     4081 cgaagacgag cgcgatgccc ggcaggatgc gtgcgagggc gcgcggcttg aagagttgtc
     4141 gctgcgcgaa ggcgaagacg agcgtgagca ggtagagcga gagcgggata acccagagca
     4201 gagggagcga ggcgatgtct gtagagaagt agttggtgac gccgagcatc aaactcgaag
     4261 gcacgaaggc gagcgcgacc cagcgcagac gcctgcgcca cgtcagagtc tcaagctgtg
     4321 tctcgtgcgc ttcaagggct tcaccaatcg atgagtttat ctctgcgtcg cgcgggtcgc
     4381 gtgcatcgag cggggcggga gttttcgtct caacttgttt cgtttgacgc cgcagcagcg
     4441 cgtaggcgca ggccgcgagc agcaggacga gcagcgcgta ggccagcgac cagaggcggc
     4501 tttgcccacg cagcgtgagc gtgggttcga gcagcagcgg gtaactcaaa agcgcgagca
     4561 ggctgcccgc gttgctggcc gcgtagagaa agtaagggtc gcgcgaagag gtttggcgcg
     4621 tctccgcgaa ccacttttgc aggagcgggc tgaaggtcga gacggcgaag aagggcagcc
     4681 cgaccgtcgt gagtaaactt ccgagcaacc agagcgaagg gttgccttcg cgcggcacgg
     4741 agcggacggt cgcttccgaa atcaggacgg gaaacgtgag cgcggtcaag agcagcagga
     4801 ggagttgcgc gccgacctgc tggcggaagc tgagccattt ggagacggcc agcgcgtagg
     4861 cgtagcccgc cagcagcatc gcctgaaaaa agagcatgca ggtgttccac acggcgggcg
     4921 tgccgcctaa gagcggcagg agcatcttcg ccaccatcgg ctgcacgaga aacaggagcg
     4981 atgcgctcgt gaacagcgtc gccgcaaata gtagcgtcag cgcgcttgag cgtctcggca
     5041 tgtgttatgc ggcggcggag cgcgcttgca gcgtggtgcg gatgagagcg ttgaattttt
     5101 cggcgtgctc ggtttgaggc atcatgcggc agcgttcgaa gagttcgagt cgtgcgcgcg
     5161 ggttgagact gagcagcgtg gcggcctgcg tgatgggcgt ggtcaaatcc tgcttgcccc
     5221 agacgagcgt gacgggctgc gtgaggcgcg caaaggcttc gcgcgtgtcg gtgttgagat
     5281 agccggagag gaaggctgcg acggcgtgct gtgcgccggg ttgatgactc gcggtgtaat
     5341 agtgcgcgac gagtcgcggg gtggcaaagc gttcgtcgta gaagagccgc ttgcgcgcat
     5401 agtcgcggag gcttcgctcg ctcgtcatcg cgttgtagaa ggatgcgccg aggacgggcg
     5461 aatggagcaa gccgtagaag gccgcgccgg gcaggtctgg gcgcgcgttg gtggcgtccg
     5521 agacgttcgg cgcgatgagc gtgagcgag
//
LOCUS       DASWNS010000166         5707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_7844, whole genome shotgun sequence.
ACCESSION   DASWNS010000166 DASWNS010000000
VERSION     DASWNS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7844"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VGB76_05805"
     CDS             <1..1067
                     /locus_tag="VGB76_05805"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0098450.1"
                     /translation="YIVHFGYAGIYMFFVISGFCIHLHWARARAAGVEQPKIDFYVFW
                     KRRVRRLYPPYLAALALYLVYNGFQHPPEVNGAYLWDLGLHLLMLHNLDLRTTYSINA
                     AFWTLAIEEQLYLAYFLLLFLRIRYGWARTLLVCAAARVGWLLLGRSLVYFTGVEIPV
                     SEAAATNWFIWALGALSVEAALGVVRLPGWCYRMWAGACALLLAMALATGLPFINHLA
                     WIHDAGWLLMHPAWGVGFFIMVNGAVRAEQRRRAVSRNASRLLVALAGLGLISYSLYL
                     THLFVLMQWYWFGFTKLHIPAISVAIMTPLSLLFAWLFFRLFERPFMSASVGLSTPSR
                     HSSSLPQQQPNEPVSDPAAG"
     gene            1132..2670
                     /locus_tag="VGB76_05810"
     CDS             1132..2670
                     /locus_tag="VGB76_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0098451.1"
                     /translation="MRHRAAAKRTALLFRCAAIGFCLSFTSSAALAKDSWAGVRSPHY
                     LLVGETNGSELREVAIRFEQFRHAFAQMFPASGLSSSVPTTVILFRDYDAYKEFRPLY
                     QGKPTEFISYFQSGLDVNYIALPLDKDRESLYVRLFHESVHLLLDSQSRRVPDWLNEG
                     LAQYYSTYEFNLAQDRNILGKPVAGNLALLRKQKLLPLETLFAVDRASASYNEREKSG
                     IFNAQSWALVHYLMNADGGRRQLQLLRFIELSAAGVPFEKSFSQSFKAAYRTIEAELQ
                     EYIRRADFRVRHNALDAHPGGVAAIETLPLTDADAHYFLGDLLLHINRYDDAAKQLQK
                     AIALNPASASAHAALGLTRVRQRQGAEARRLLQRAVSLEPDNYLMHYYYAYALSREEM
                     DDEQEITEYEPETAQIMRRELRRAIALNPGFTESYRLLAFVNLVTGEQLPEAVELLRH
                     GLKLSPNRPDLTFILAQVYLRQKDSHAARATLRQLINTNANPRLRARAQTLLERIKTG
                     GAAR"
     gene            2694..3653
                     /locus_tag="VGB76_05815"
     CDS             2694..3653
                     /locus_tag="VGB76_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098452.1"
                     /translation="MNQLCDKIDEFLEVELKAHPAQVEQFFERVQLNYDEAIRAATRY
                     LLLLIVAWFVTYAIHATWITKIEWAGLEFKPEMTIASPLLIGLLSYGLQSALAGAVVM
                     WEAISRRLRYTLPTAWKYKLDDLLAPPTFSNVERMLEPESEFKTFSLCSRGWFVLVTV
                     LIFGGSIAALVHTTYLLTRVSPPVARWVVIAISAALGAVAWIRGVVLSASAIEATGGF
                     QLCHHRGTRTVPPPDEELPRDNLKGSPADSPVDSVEDSVEDSVEDSVGDGVEDNSSAS
                     PAPSAQPPPSTIHPGVAPALIYSRARLFDLKRADVVILVERKK"
     gene            complement(3730..5304)
                     /locus_tag="VGB76_05820"
     CDS             complement(3730..5304)
                     /locus_tag="VGB76_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0098453.1"
                     /translation="MSGRSGLRAPSAWCARTFSLPLILILSFNGLGQVRPSNADASSP
                     RNASLAALDAYVAELSKRLPLTPGLSIAVASGERVVYARGFGFRDLKAKLPVTPQTQF
                     YIASTTKSFTATAAKLLADEGKFDLDAPLKKYFPNLTLKTPLSAEQITIRDLLTHRSG
                     ISNEAINFRTAYTGQHDAGVLLELLASYSKPVSPEFRYSNIGYLITALAMEKATGETW
                     QQIVERKLFQPLELRSTTAYASKARASGNFALPYLANANGTFDELPYKEDSTMHAAGG
                     ISSSAEDLAKWLVMNMNGGRYGGRQIIPASSLEEVLAPQINQKRDFYRFKRYGYSLGW
                     NIGTYNGDKFIHSFGEFHGFRPHVSFMPERKLGVVVLVNESTDAFTLPDLIACDIYDH
                     LLGRKTLNATTNPEIDKYLAGLQKGKEERASRKAASGAAGGATEKKSEPTLPLAAYAG
                     EYENPEWGKISINLNAGTLALHFGVINSTLSHLTGDTFEARLRPGDASRLTFKVDANT
                     GVTALSVVGQNFARVR"
     gene            complement(5416..>5707)
                     /locus_tag="VGB76_05825"
     CDS             complement(5416..>5707)
                     /locus_tag="VGB76_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0098454.1"
                     /translation="HATFAEQVTLAGVAGAVGVHPVYLAARFRKHYQCTVGEYVRRLR
                     VEVACREVVRTDRALSEIAPSVGFYDQSHFTNVFRRHTGMTPAQYRAAFRSR"
BASE COUNT         1081 a         1761 c         1674 g         1191 t
ORIGIN      
        1 cctacatcgt ccacttcggc tacgccggaa tctacatgtt cttcgtcatc tccggcttct
       61 gcatccacct gcactgggcg cgtgcgcgcg ccgccggagt cgagcagccg aagatcgact
      121 tctacgtctt ctggaagcgg cgcgtgcgtc gtctctaccc gccttatctc gccgcgctcg
      181 cgctctacct cgtctacaac ggcttccagc atccgcccga agtgaacggc gcttacctct
      241 gggacttggg actccatctg ctgatgctgc acaacctcga cctgcgcacg acctacagca
      301 tcaacgccgc cttctggacg ctcgccattg aagagcaact ctatctggcc tatttcctgc
      361 tgctctttct gcgcattcgc tacggttggg cgcgcacgct tctcgtgtgc gcggcggcgc
      421 gcgtgggctg gttgttactg gggcgcagtc tggtttactt cacgggcgtg gagattcccg
      481 tgagcgaagc ggcggcgacc aactggttca tctgggcttt gggcgcgctc agtgtggaag
      541 ccgcgctcgg agtggtgcgt ctgcccggtt ggtgttaccg gatgtgggcg ggcgcgtgcg
      601 ccctgctgct ggcgatggct ctggcgacgg ggctgccgtt catcaatcat ctcgcgtgga
      661 ttcacgatgc gggctggctc ttgatgcatc cggcgtgggg cgtcggcttc ttcatcatgg
      721 tgaacggtgc ggttcgcgcc gagcagcgac ggcgggcagt gtcgcgtaac gcttcacgcc
      781 tcctcgtcgc gctggccgga ctcggcctga tctcttattc gctctacctg acgcacctct
      841 tcgtgttgat gcagtggtac tggttcggct tcaccaaact tcacattccg gccatctcgg
      901 tcgccatcat gaccccgctc tcgctcttat tcgcgtggct cttcttccgt ctcttcgagc
      961 gacctttcat gtcggcatcc gtcggcctga gcacgccttc gagacacagc agcagcctgc
     1021 cccaacagca gcccaacgaa cccgtcagcg atcccgctgc gggatgaact atcagacgtt
     1081 ctaactaacc acccggcgcg aggcaacttc aacttgcgag aatcgagacg gatgagacac
     1141 cgtgcagcgg cgaagcgaac ggcgcttctc ttccggtgtg cggccatcgg attctgtctc
     1201 tcattcacct cgtccgctgc gctggcgaaa gactcatggg cgggcgtgcg ctcgcctcac
     1261 tacctgctgg taggcgagac gaacgggagc gagttgagag aagtggcgat ccgcttcgaa
     1321 cagtttcgcc acgccttcgc gcaaatgttc cccgcctccg gcttgagttc ctccgtcccc
     1381 acgacggtca tcctgttcag agactacgac gcctacaaag agttccgacc tctctatcag
     1441 ggcaagccca cggagttcat cagttatttt caatcggggc ttgacgtgaa ctacatcgcg
     1501 ctgccgctcg ataaggatcg ggagagtctc tacgtgagac tcttccacga atccgtgcac
     1561 ctgctgctcg acagccaatc gcgccgcgtg cccgactggc tcaacgaagg gctggcgcag
     1621 tattacagca cctacgaatt caacctcgcg caagacagga acatcctcgg caagcccgtc
     1681 gcgggcaacc tcgcgctctt acgcaagcag aaactcctgc ccctcgaaac cctgttcgcc
     1741 gtagaccgcg cttccgccag ttacaacgag agggaaaaga gcggcatctt caacgcgcag
     1801 tcgtgggcgc tcgtgcatta cctgatgaac gccgacgggg ggcggcgaca gcttcaactc
     1861 ctgcgcttta tcgagctgtc tgcggcgggc gtgccgttcg agaagagttt cagccaatcg
     1921 ttcaaggccg cgtaccggac tatcgaagca gagttgcagg aatacatccg ccgcgcagac
     1981 tttcgggtgc gccacaacgc gctcgacgcg caccccggcg gcgtcgccgc aatagagact
     2041 ctgccgctca cagacgccga cgcgcattac ttcctcggcg acctgctgct ccacatcaac
     2101 cgctacgacg atgcggcgaa gcaattgcaa aaggccatcg cgctcaatcc cgcatcggcc
     2161 tctgcccacg ctgcgctcgg cctgacgcgc gtgcggcaga gacagggcgc ggaagcgcgg
     2221 cgtctgttgc aacgagccgt cagcctggag cctgacaact acctcatgca ctactactac
     2281 gcctacgccc tcagccgcga agagatggac gacgaacagg agatcaccga gtacgaaccg
     2341 gaaaccgcgc agatcatgcg ccgggaattg cgccgcgcca tcgcgctcaa ccccggcttc
     2401 acagagtcct atcgtctcct cgccttcgtc aatcttgtca ccggcgaaca actccccgaa
     2461 gccgtcgaac tactccggca cggactcaaa ctctccccca accggccaga cctcaccttc
     2521 atcctcgcac aggtctatct acgccagaaa gattcccacg ccgcccgcgc caccctccga
     2581 caactcatca acaccaacgc caacccacgc ctccgcgccc gcgcccaaac tttactcgaa
     2641 cgcataaaaa ccggcggcgc ggcgcgttaa gcatcatcag gagatacacg agtatgaacc
     2701 aactctgcga caagattgat gaattcttag aagttgaact caaagcgcac cccgcgcagg
     2761 tcgaacaatt cttcgagcgg gtacagttaa actatgacga agccattcgg gctgccaccc
     2821 gttacttact cttgttgata gtcgcatggt tcgtgactta tgcgatccat gcgacatgga
     2881 tcacgaagat cgagtgggcg ggcttagaat tcaagcccga aatgacaatc gcctcacctc
     2941 tcctcatcgg cctgctgagc tacggattgc aatccgctct ggcgggagcg gtcgtcatgt
     3001 gggaagcgat tagccgacga cttcgctaca cactgccgac ggcgtggaaa tacaaactcg
     3061 acgacctcct cgccccgccg acgttttcca acgtcgagcg aatgctcgaa cccgaaagcg
     3121 agttcaaaac attctcgctc tgttctcgcg gctggtttgt gctcgtcacc gtgttgatat
     3181 tcggcgggag catcgccgca cttgtccaca cgacctattt actcacccgc gtctctccgc
     3241 ccgtcgctcg ctgggtggtg atcgccatca gtgcggcttt gggcgcggtg gcgtggatta
     3301 gaggtgtagt gctctccgca tccgcaatcg aggcgaccgg cgggtttcag ctttgtcacc
     3361 accggggaac gcgaaccgtg ccgccacctg acgaagaact tcccagagac aatctgaaag
     3421 gcagcccggc agacagcccg gtagatagtg tggaagatag tgtggaagat agtgtggaag
     3481 atagtgtggg agatggtgtg gaagacaatt catccgcgtc gcccgctccc tctgcgcagc
     3541 cgccaccctc gaccattcat ccgggtgtcg cccccgcgct catctacagc cgggcgcgtc
     3601 tgtttgactt gaagcgggca gatgtcgtga tcctcgtcga gcgtaagaag tgacggggcg
     3661 gctcgaatgg caggtcgcag cgacgcccct cacaactctc tcaagtctga cttttacatt
     3721 tcgcgcaccc tatcgcacgc gggcgaagtt ttgtccgacg accgacaggg cggtgacgcc
     3781 cgtgttcgca tcaactttga aggtgaggcg ggaggcgtcg cccggtctga gtctggcttc
     3841 gaaggtgtcg cccgttaaat gggagagggt tgagttgatg acgccgaagt ggagggcgag
     3901 cgtgcccgcg ttcaagttga tgctgatctt tccccattcg ggattttcgt attcgcctgc
     3961 gtaggccgcg agcgggagcg tcggctcgct cttcttttcg gttgcgccgc ctgccgcgcc
     4021 ggacgccgct tttctgctgg ctcgctcttc ttttcccttt tgcaggccgg cgaggtattt
     4081 atcgatctcc ggattcgtcg ttgcgttgag ggtcttgcgc ccgagaaggt gatcgtagat
     4141 gtcgcaggcg atgaggtctg ggagcgtgaa ggcgtctgtg ctttcgttga cgaggacgac
     4201 gacgccgagt ttgcgttcgg gcatgaagga gacgtgcggg cggaagccgt gaaactctcc
     4261 gaaggagtgt atgaacttgt cgccgttgta agtgccgatg ttccagccga ggctgtagcc
     4321 gtatcgcttg aagcggtaga agtcgcgctt ctggttgatc tgcggcgcga gcacttcttc
     4381 gagcgacgac gccggaatga tctgccgtcc gccgtacctg ccgccgttca tattcatcac
     4441 gagccacttc gccaaatctt ccgcgctcga actgatgccg cccgccgcgt gcatcgtgct
     4501 gtcttccttg taaggaagtt cgtcgaacgt gccattggcg ttcgccagat agggcagcgc
     4561 gaagttgccg ctcgctctcg ctttcgaagc gtaggcggtg gttgatctca gttcgagcgg
     4621 ctggaaaagt ttgcgctcca cgatctgttg ccacgtctcg cccgtcgcct tctccatcgc
     4681 cagcgcggtg atgaggtaac cgatatttga atatctgaac tcgggcgaga cgggcttgct
     4741 ataacttgcg agcaattcca gcaggacgcc cgcgtcgtgc tggcctgtgt aagcggtgcg
     4801 aaagttgatc gcttcgttac tgatgccgga acgatgcgtc aggagatcgc ggatggtgat
     4861 ctgctcggcg gagagcggcg ttttcaaagt gaggttcggg aaatactttt tgagcggcgc
     4921 gtccagatca aacttgcctt cgtccgcgag cagtttggcg gcggtagcgg tgaaagattt
     4981 cgtggtcgaa gcgatgtaga actgcgtctg cggcgtgacg ggaagtttcg ctttgagatc
     5041 acgaaagccg aagcctctgg cgtagacgac acgctcgccg ctcgccaccg cgatgctcag
     5101 gccgggcgtg aggggaagcc gcttcgacag ttcggcgacg taggcgtcga gcgcggcgag
     5161 cgaggcgttg cgcggcgaag aagcgtctgc gtttgacggt cgcacttgtc ccaatccgtt
     5221 gaagctcaaa attaagatga gcggcagact aaaagttcgc gcgcaccatg cggacggcgc
     5281 gcgtaagcca gagcgtccag acatattttc ctccgttgac gacgacagcg atgacagcaa
     5341 agcggcaacc cggaagaaca gtttattagt tagttaacat tccgtcttgg ataaacggaa
     5401 ttaatttgga gcagattaac gcgaacgaaa ggccgcgcgg tattgcgccg gagtcatccc
     5461 ggtatggcgt ctgaagacgt tggtgaagtg gctctggtcg tagaagccga ccgagggggc
     5521 gatttcgctg agcgccctgt ccgttcgcac cacttcgcgg caggcaactt cgacgcgcag
     5581 gcgtcgcaca tactcgccca cagtgcattg ataatgtttg cggaaccgtg ctgcgagata
     5641 gaccggatgc acgcccaccg ccccggccac cccggccagc gtgacctgct ccgcaaaggt
     5701 cgcgtgg
//
LOCUS       DASWNS010000167        10817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_369649, whole genome shotgun sequence.
ACCESSION   DASWNS010000167 DASWNS010000000
VERSION     DASWNS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369649"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VGB76_05830"
     CDS             complement(<1..283)
                     /locus_tag="VGB76_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0098455.1"
                     /translation="MSEQSGRVYEFGPFVLDPDRDILLKGGEPVALPPKAFALLVALV
                     RAGGRTVTKDEILREIWPDTAVEEQNVGQNIHLVRKALSDEADRPSYVVT"
     gene            702..1733
                     /locus_tag="VGB76_05835"
     CDS             702..1733
                     /locus_tag="VGB76_05835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0098456.1"
                     /translation="MAKPVADLRDYNGTYYGASGTGTIGYTTLYRDNEDKGAASGCNG
                     EGCGRHPGVDIPVGSGTAVYASLSGTVVRSEDNGCAGTGWGGLIVIRSTNPWNPNETL
                     YFTYAHLKTRYYSMGQTVTTSTQIGLSGGKPNSDFCPGNSTNSHLHFQIDRNHGTTIP
                     YFPATTTELNQRDSDFRVTAKTYNPIVFVTGGYRWSFNKPGERELWEIVNLQNWGVSN
                     DALWVNGVGDTYVRRGGDVNCGLAKPCSSSISAEASMYPKVYLDLYNVCFSNPGKIYF
                     TTSSDPIWSETKSVSYYPSGQGPYREHIYMNQNSEWKGIITGLRIDPAVSCSSGDDPT
                     YYGEITIER"
     gene            6958..8046
                     /locus_tag="VGB76_05840"
     CDS             6958..8046
                     /locus_tag="VGB76_05840"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0098457.1"
                     /translation="MKIYRPLFNLVSRERLRKLLNQKFTLPQLLNRVKYEMSDKGKAL
                     LVFRPTWMTVYVSDRCNLACGFCVNQSPDRANAGLGFHEPLQDMTLARLREILDKTPE
                     IMVVTLAGIGEPTVNPEIYDMAREIRGRHMRANLITNGVLLKRDADKVLSAGFNEILV
                     SVNAVNESAFAAVVNKPPHTFGVVIDGVRAIIQRRGRRSEPKVRIAFVCTKSNYRQMH
                     DFISLGKELRVDGVRFHNLITDGMKRYEIDQCLFEDDQDVVQYIRDLKEAGHGSGWPE
                     VEFPPLLPRHVQERKCQWYHKNLCVDSNGSVSGCGRFIVPRPEYGNVLTNRDAWNGQH
                     FRDMREMFSDSTIPLLSCCQHCVENARQ"
     gene            8150..9289
                     /locus_tag="VGB76_05845"
     CDS             8150..9289
                     /locus_tag="VGB76_05845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0098458.1"
                     /translation="MTSTFDITLVTDRREFDTLAESWQTLADSSPTASVFSTWYWISA
                     WWKHFGDGVSPFIIVARDTEGNLVGVAPLVRHRRGPFRVLKFLGAPHSDYHEFLFRPH
                     EQPLISAAICKFLLGLRDEWDVWEVNDVTASSPLWPLIEEVFADEFEIHPLRDITCPY
                     AALPDDWGTFLGGLSRKRGRYIRNRLEYLERIGVGYRTATRKDAITETMDHLYLMHVE
                     RWRNRKCLNPVHGVPHFLPFLKEAAMNFNEAGCLALAEITTGHRPMAIALNFRYKTKV
                     YNYLRGFDIAWHSYSPGLLLDVLAIKQAIESYCREYDFARGDEDYKRTISNRLRVNRH
                     AFVTRRAKSDLMLAAFVRSCVVWDKLAHRRGTGRLIRFMSKYSAR"
BASE COUNT         2371 a         3085 c         3007 g         2354 t
ORIGIN      
        1 ctgtcaccac ataggagggg cggtcggcct cgtcgctgag cgccttcctc accaagtgga
       61 tgttctgccc gacgttctgc tcctcgacgg ccgtgtcggg ccagatctcc cgcagaatct
      121 cgtccttcgt gaccgtgcgc ccgccggccc ggacgagcgc caccagcagc gcgaacgctt
      181 taggaggcag cgcgaccggc tccccgccct tcagcagaat atccctgtcg gggtccagca
      241 cgaatgggcc gaactcgtat acccgcccgc tttgctcgct cataacactc ctaacgaacc
      301 cctaacggaa acctgatcga accctaagga cttcgcggga agccgtcgcg catcctttgc
      361 cgcggctgaa gtgatcgtgg gagatgaccg gcggaaagag tgcgtcggga gtcgctccga
      421 ttataacgaa aacccgcgac gccacgaagg gagcaccggc gactcccgac cgcgcccgca
      481 gttctttcgg cggcggccgc ctgacgaagt caacagaaag gaagagtatg ctgaaaacgt
      541 tttcgatgat ccgcctgacg gttctggcgg cctgtctgat cctctcccta gtcctccccc
      601 tggcgggcac gaccgcggcg cccgaaggtg gggtgaccgc cgggggcacc ccgaccgccg
      661 ccccgagcct cgccagagcc ccgagcctgg agcccacggg gatggccaag cccgtcgccg
      721 acctacgcga ctacaacggg acttattacg gcgcgtcggg aaccggcacc atcggctaca
      781 cgacgctcta ccgagacaac gaagataagg gagccgcgtc aggctgtaac ggcgagggct
      841 gcggcaggca ccccggcgtg gacatccccg tcggcagcgg aacggcggtc tacgcatctt
      901 tgagcggcac tgtcgtccgc tccgaggaca acggctgcgc aggcacgggc tggggcggcc
      961 tgatcgtcat caggtcaacg aacccttgga acccgaacga gactctttac ttcacctacg
     1021 cgcacctgaa gacgcgctac tactcgatgg ggcagacggt caccaccagc acgcagatcg
     1081 gcctcagcgg cggtaaacct aactcagact tctgccccgg gaattcgacc aactcgcacc
     1141 tgcatttcca gatcgacagg aatcacggga ctaccatccc gtacttcccg gcgaccacca
     1201 cggagttgaa ccagcgcgac tccgacttcc gggtcaccgc gaagacctac aaccccatcg
     1261 tcttcgtgac gggcggctac aggtggagct tcaacaagcc gggtgagcgc gagctgtggg
     1321 agatagtcaa cctacagaac tggggcgtct cgaacgacgc cctctgggtc aacggcgtcg
     1381 gggacaccta cgtccgccgc ggcggtgacg tgaactgcgg cttggcgaag ccgtgcagca
     1441 gcagcatctc ggccgaggcg agcatgtacc cgaaggtcta ccttgacctc tacaacgtgt
     1501 gcttcagcaa ccccggcaag atttacttca cgacctcttc cgaccccatc tggagcgaga
     1561 cgaagagcgt gagctactac ccgtcggggc agggcccgta cagggagcac atctacatga
     1621 accagaacag cgagtggaaa ggcatcatca cggggctgcg gatcgacccc gcggtgagct
     1681 gctcgtcggg cgatgatcca acatactacg gcgagatcac catcgagcgg tagcctctgc
     1741 gggaaggtag aagcgacggt tgcggctcta cgctccgccc cggggcgcca tttgagatcg
     1801 gcgccccggg tgcggtctat taaagacaga aagggcggcg aacttactca tacttcgaga
     1861 agaactcggc gttggccccc ataagtgccg cactgcatca ctcctaattc tcgactttgg
     1921 ggaagcatta ccactttgtg tttagtgaga aactcttcgg tctggtttgt acactgctcc
     1981 ctcgtcaaca ttcctcttgg gaggccttgc ccaacaatta agcgctgcct atcacatatt
     2041 gtgatgacat cttccaacct actcaagatg aagcggctat tttggattgt tgggcaaggc
     2101 tcctcttggg atatggttct gttgaggcag accacaggcc cgttaaagtc ttcgcggtag
     2161 cggtacactt ggttgaagcc cgccgcgatc ttctcggtta gagcatacgg atgggcttgg
     2221 ccgtctggag cgcttcaagt ctcctaccgc tctcgtggcg tccttctcct cctacacgct
     2281 cgggatcaaa cattctctca gacgcaaaat cattgacagt gccacgcgat tgttataaaa
     2341 agcggctgtt ttggttttac tcattttcct ttgagacggc ctttgcggct ttcctcaccc
     2401 ggttcgtccg tgacggatcg gatgtccaaa gcagcaccag ccaagagaac tacccatcac
     2461 ctttagcaag agctgtttgc agcagggcag cagatttcat taaccgtact aacacgctgc
     2521 tgtagtgatt gccctggctc agtctctaag acctccctca gctcggcccc aatggcgagc
     2581 caagggtggc ctttatttct gcaagggtgg ctcgagtgga aaaactactg aacggctttg
     2641 aagccgcatt ccaagttctg ggcgagtttt cagtagggag taaggacgcc ctgtgggatg
     2701 aggcggatag ctatgcctga cgagaggtgc atgtcaaaga gtaacaatga ccgttaagaa
     2761 ggttgccatt ttggggtgag ggatagcgga atgttgcttc gcgaggagcg cgtatttatg
     2821 tcggacgctt aagctatccc cttccttcgg taccctctca ttgagtcgta agctcttacc
     2881 tctggccaga tttccgctaa cttttccgca ataactcacg cgggggagaa gagtctttct
     2941 attatgagaa tccttattgt tacccctgaa ccggcatccg ctcttaaacc gcgtgttttt
     3001 cggttcgcta aactattaag taccgcacat gtcgttcaca tcgcgtatat cgacgaaatc
     3061 caggggagac aagtgccaac tgatattcgt gttgacggcc aaaatcagct acaagaagta
     3121 gggattgaaa cacaccaact tcgcgcgtgc tggccactcg ctattgtgcc attactggct
     3181 aattacatgc gcggtctgcc ccttcgagtg ggcttgtacg cgcagggtaa gatccgttca
     3241 caagttgagc agcttgttgg caaggttcgc cccgacctga tacatgtaga ccgcgagaga
     3301 gcattccctg ttgtttgtca cttaaggata ccgctcgtcg tcgacctcac cgatcctgtc
     3361 ggctggtact tagaacagcg ctcgcggtta actcatcctc tgctatccga gcttctacgc
     3421 atagaggcga gaagaatgtg gctctacgaa gggcacattg cgaagcaggt tagagagata
     3481 ttctttgcgt ctgaattcgg ggccagtagg ttcaaggata gataccccgg cgcgaaggtc
     3541 ggcgtaatcc cagtccccct cccgcccccc gcaacggctg cgtcatcatc acccctcttg
     3601 ccgggggggc cgccccatat atgttttttc ggaaacctat actacccgcc taacgtccaa
     3661 gccattacag atttcgtcaa gaattgttgg ccggaagttc gcgcccgact tccaaccgcc
     3721 accctgcatg tcgctggcag ccgaccccac agtgcaatca cgaaactgca gcgccgtgaa
     3781 ggggtttacg ttcattcaaa tccggtcgag atgggctcgc tcttgcgagg ctgcgatgta
     3841 gccgtcgcgc cacttgaagt gtgtgcagga ttccccaata agatcgttga cgccgtcatt
     3901 acaggcggcc tcccagtcgt cgcctcatcc gcagcccaag ccggattacc ccctgcggcc
     3961 cgggatacac tccctacggc ttcggagccg cgtgaatggg ctgagaagat tagtatcctc
     4021 tggcaaagga aggatctgcg acaagattta gtgaacaaaa tgcgggacgc gctcacctca
     4081 acccttaata atgagttaat tctgtcgcag ctgaatacca cttataactg tgcgctgtcc
     4141 tcgtaaacgg ctcgtgtgcc gcctgaccat gttatgccaa cgtacttaag ttcacccaac
     4201 cgagttattt acacgccgac ccaaaggcta cctttcaccg gcgcaagcac ctacgcgtcc
     4261 ggcaaagagc atagatacag caacgagtcg ctccctcttt gacgtttagc cgacatgtct
     4321 acacacattc aagtatctcc ccctgctagt ctaagtgcac gccgcatgtg ggcactcacc
     4381 aatcttgagc gtgtcctggc ctttctattc gccattctct tagctccgct tttcatagcg
     4441 gtcgccatta tcattgtttg cgtatcaaga agaactccac tcgtagccca cctccgtgtt
     4501 aaacagtatg gggaaccttt ctggatgctc aaattccgta cgatgtggca taagcctgcc
     4561 agcaagtctt tcctaaaggt agtagagttc attgctgacg aggcaggccc cttatctaaa
     4621 aagataggag acgaacgagt tagcagtcga ctcgcgcggt tgtgtcggca gttttcgata
     4681 gatgaattgc cacaactgat acatgtggcg gcgggtgaca tggctttagt cggcccccgg
     4741 cccctcacca ggacagagtt gctcactcac tacgggcctc acgcccgaga ggttttaggc
     4801 gtaaaaccgg gactcacagg gctgtggcag gttatgggcc gtaattccct tacctaccga
     4861 caaaggcgca ggctcgattt attcttggta cggagtagat ccgcgaggct atacgcaatc
     4921 atcctcggtc gtacatttta tcaagtattt agcggtaagg gcgcatggtg acaatccccg
     4981 cctcaaggat acatttagcg actaccgagg tgcgctggcc agtcagcact gtaacattca
     5041 ctatgccgga gccccttcga caaggccgtc atatacatgc gccgctgcat aacatgtaca
     5101 gggttaccgt ttatgggcca cagggcgaaa gagggcaccc cctcgtcccg ttcttgtata
     5161 agcaattccc ggaacctgct cagaaaggac aatctcccgc ctttgattcc cctacctccc
     5221 tcgcggctcg ttttgctccc cacacaaacg gtcagtacaa gctgtcgctg gaggtattgg
     5281 agggttcgtg gaagaattac gagttccgcc gaattaagat ctcagacctt ccagatgata
     5341 tggatgtctc gccgcggttc cgcatgggag taaacatggg gtggcacgcc agcgaatcag
     5401 gtgacgagcg ctttgggatg tacgatcttt ggctatccaa gtttgcccgg gccgggggag
     5461 ggctggtccg gataatgatg acaagctggg gattcagcat cgccccggtg gactactccc
     5521 cttgcccaga gatctccgtc cgcctcgaca gaggctggca attggatcga gtgctggacc
     5581 ggtgctcgga gttagatatc gcggtggtgc tatgcctctt cacgttcgat gatttcaaag
     5641 aaggctcagg ctgggactgg cggattaatc cgtcaagtat tcaccggggt ggccattgcc
     5701 gcactccaat cgagtggttc acccagcccc aagcattaga ctgggtcgag agattgctca
     5761 tcgctatcgt ggcgcgttgg ggagcttaca aatgtattgc cgcttgggaa ctattcaacg
     5821 aggtcgatca ggtcgacgga taccgccggg ggattatccg gcactggtat cagcgtatcg
     5881 cagaccacat acagatacac gacgcagtga cgaggcccgt tctcctaagt cacgcgaacc
     5941 cacaggaggc cgtagaacag agcagggccg gggcgccctt ccgccagata cacacatatg
     6001 gttggcccag ctcgtgttcc gcccggaata gcatttattg ggccaactgt ctgcggatcc
     6061 gcgagaaagg cgatggcctt atcagcgagt tcgcgccccg atctgacagg ccgcccaccg
     6121 aagaagatcc tgagggcgag gaaatgcgca acacactctg ggcttcgtta ttcgccggct
     6181 ggttgacacc ggcatggcct tggtactggg actcgcacat cgaggcgcgc aacctctggc
     6241 gcgtctggac agggctgaac aagttttgcg ttgcgatcaa ctggcttgaa gccccttggc
     6301 atcccgccgc cgcagagctg tgcgttctta agagcgatcg gctggatgct tcggcgcaca
     6361 aagcagaagc gggtagaagg agactatacc tgcgctcccg gctcgcccac ttattccgga
     6421 acaaggcatt ccgaaaaatt gcctacgccg cttttcacca tttgcgtttc agaatactaa
     6481 ccgacctcgg tggtccgtgt tgcgtgctgg cgctatttcg gcacagcgct ccctcccctc
     6541 cccaatgtgt cgcttgggtt cacaacacta ccgccgggca gcacacaaca cccagaagac
     6601 agagtgtcgt actgaagctg aatatcggga gcgccggagg caggtgtcta gtcagattct
     6661 gggatacatg tagaggggaa gcgaccgagt acctgcgagt caatcatgcc agaggagctc
     6721 ttcgtatcga gcttccctgg tttgaacgcg acttggccct agatttgaag ttcattaacg
     6781 ggcctgacgc cgatgcgcga gataaggatt gtcacacaag aggacaaaac gaacttaatg
     6841 gcggaattgc cccgccaaac tttcagcaat ccggctttat cgtcggcacg tctccaatcg
     6901 gtgtgatcgc ccctgtgcgg gcgtgccttg gggcgggtgt gccacaggag gtgtgagatg
     6961 aaaatttacc gacccctttt caatctcgtc agccgggaac ggctcagaaa attactcaac
     7021 caaaagttca ctctaccgca gttgttaaat cgcgtgaagt acgagatgag cgataagggc
     7081 aaggcgctgc ttgtatttag accaacttgg atgaccgtct acgtttccga tcgatgtaat
     7141 ctagcctgtg ggttttgcgt aaaccagtcg ccggatcgcg ccaacgccgg gttaggtttt
     7201 cacgagccgc tccaagatat gactctcgct aggctaagag aaattttaga taaaaccccc
     7261 gagatcatgg tcgtcactct cgcgggcata ggcgagccga ctgtaaaccc ggaaatttac
     7321 gacatggccc gcgagattcg tggacgccac atgcgcgcca acctcatcac taatggggtt
     7381 ctattgaaga gagacgctga caaggttttg agcgcgggtt tcaacgaaat cctcgtcagc
     7441 gttaacgcag tcaacgaatc cgcttttgcg gcagtcgtta acaagccgcc acacacgttc
     7501 ggcgtcgtca tcgatggtgt aagggcaatt attcaaaggc gcggacgccg cagtgaaccg
     7561 aaggtacgta tcgcgttcgt ctgcacaaag agtaattata ggcagatgca cgacttcatc
     7621 tcgctcggca aggagcttcg tgtggatggg gtcagattcc acaacctcat caccgacggc
     7681 atgaagcgct acgagatcga tcagtgcctg ttcgaagacg atcaggacgt cgtgcagtac
     7741 atcagggact tgaaagaagc cggccacggg tcgggatggc ccgaagtcga gttccccccg
     7801 ctcctcccgc gccacgttca ggagagaaaa tgtcagtggt atcacaaaaa tttgtgcgtt
     7861 gactctaacg gcagtgtatc gggttgcggc cggttcattg taccccgtcc cgaatacggt
     7921 aacgtcctta cgaataggga tgcgtggaac ggccagcact ttcgggatat gcgtgaaatg
     7981 ttctccgaca gcacaatccc actcctatcg tgttgccagc attgcgtcga aaacgcaaga
     8041 cagtgagagt ggcgttctct aatgaccggg ttcggtaccg gataaggacc gcgcgcgagc
     8101 gttacggcta gacgtgtcag tgtactcaac gcgcctgcgt cattaaactg tgacatcaac
     8161 tttcgacata accctggtaa ccgaccggcg cgagttcgac acattggctg agtcctggca
     8221 gaccttagcc gacagctcgc ccaccgcatc cgtgttttcg acgtggtatt ggattagcgc
     8281 gtggtggaaa catttcggtg acggggtcag ccctttcatc atcgtagccc gcgacacgga
     8341 agggaatctc gtgggcgtcg cccccctcgt acgtcaccgg cgaggcccat ttagagtttt
     8401 aaagttcctc ggcgcgcccc acagcgacta tcacgagttt ctgtttcgcc cccacgagca
     8461 acctttgatc tcggcggcaa tctgcaaatt cttactcggc ctacgagatg agtgggacgt
     8521 gtgggaagtt aacgacgtta cggcctcatc accgctttgg cctttgatcg aggaggtttt
     8581 tgctgacgaa ttcgagatac atccgctccg tgacattacc tgtccatacg ctgcgctgcc
     8641 ggacgactgg ggcaccttct tgggcgggct ctcccgcaaa cgcgggcgct acatacgcaa
     8701 caggctcgag tatctagagc ggataggcgt cgggtaccgt acagccacga gaaaagatgc
     8761 aatcacagaa accatggatc atctttactt aatgcacgta gagcgctggc gtaacaggaa
     8821 gtgcctgaac ccggttcatg gggttcctca cttccttccc ttcctgaagg aggcggcgat
     8881 gaactttaac gaggcgggct gtctagccct agcggagatt acgaccgggc accggcccat
     8941 ggctatagct ctcaattttc gctacaaaac caaggtctat aactacctga ggggtttcga
     9001 catcgcttgg cactcgtata gtccgggtct gcttctagat gtcctagcga tcaagcaggc
     9061 gatcgagtct tactgccgcg aatacgactt cgcccgcggg gatgaggatt acaagcgaac
     9121 aatatcgaac cgcctgaggg tgaacaggca cgccttcgtc actcggagag ccaaaagtga
     9181 cctgatgctc gctgcgttcg tgcgttcgtg cgtagtgtgg gacaagcttg cccaccgcag
     9241 gggcacggga cgattgatcc gatttatgtc gaaatattcc gctagataat ggccatggtc
     9301 accgtcatgc attccgtcat catcccgacg cgcaataggg cggcgcgctt gagacaactt
     9361 ctggacgctt tggtgcggca agcccataac gcgtccggcg tcgaaatcgt ggtcgtagat
     9421 agcatgagca cggatgacac gagggacgtg gttacttcct acacccgcca atacgagttc
     9481 atcagaggtt gcgtcgcagc ggaggtcggc ggatccgcgg ccaggaatgc agggatcaaa
     9541 acctcgttgg ggagggtctt gagcttttta gacgatgacg tccttccgtc ccctaggtgg
     9601 cttagggcaa cgacgggcgc attcgagaac cccacggtgg cctgtgtggc cgggcgggtt
     9661 aagatcaact ggacggcacc ccgtcctccc tggctgccag ataaattcct gtacttcctc
     9721 ggcgaaaagg attacggcga aacctcaaga gagttggagt ggggggagta tcccggggcc
     9781 gcaaacgtcg cgttccgcag ggaagatatt gttcggctgc ggggatttcc aacacacttg
     9841 ggacatcatg gggacgcaat cggtgcgaac gaagaagttt atttgtgcaa ggagttacgc
     9901 agacaagggg ggaaagttta ttacgtaaga gaagctgagg tcacacactt tccgcctccg
     9961 cactacgtga ccagagaata tttcctcggg cgggctaggg tccagggggc cgcaaacgct
    10021 caattggaca tgatgttcgg ccggacattc cggtggctcc gcctagggaa gagcgtactc
    10081 ctacatccgg tcgaccatct tgcaagtcga gtcttccacg cgctcgatcg tccggccctg
    10141 gctttcaagc atcggtgtga tttggagaag tctcgcgggt acctctcgtc tttctatcga
    10201 ggctttcaag cagcgggtgc taatccagga ccgctcaacc attgatgatc acgatttatg
    10261 atacctccac agcaggctcg gaaagtactt gtggtcgcct atcattaccc tccttccggg
    10321 agtgtaggaa ctcgtcggac gctaaatttt attcgccacc tgcctgaatt cgggtgggcc
    10381 ccgctggtgc ttactgtctg tgaagattac tactcccagc atgatgacgg gggcttaaat
    10441 gatggcctgc caccaccacc cgtttaccgc actcccgtct ttagccccga acggctttta
    10501 cggcgtcggg gtcgagatga aaacaggctt caggctcatg cagcaccaac agcggcagca
    10561 cgtcggcgaa atctagggtc tgtcataaag gggatactgc acgcggtggc tataccagac
    10621 gaggtagctg ggtggctccc ctttgcgttc ttccgcgggg ctagggtcat taacaaggaa
    10681 agggtggaac tcatatacgc cacatccccc ccgccgacca ctttaattgt gggggtgtgt
    10741 ttgtcgctca tgtctaacag acccttagtc gtggatctgc gtgacaactg ggcggtatat
    10801 cccgattggc tatattt
//
LOCUS       DASWNS010000168         6355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_111534, whole genome shotgun sequence.
ACCESSION   DASWNS010000168 DASWNS010000000
VERSION     DASWNS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111534"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2267)
                     /locus_tag="VGB76_05850"
     CDS             complement(<1..2267)
                     /locus_tag="VGB76_05850"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0098459.1"
                     /translation="MKLSTGLTRFPNSARRIMFFLCAALLILNAQAIARAQTSSTSTV
                     SGRIIDSNGEGLAGAEVELRNVATGLVQKQQSNSLAHYVFSPVLPGEYALVVRMQGFR
                     QASFSDLKIEVAKSYSFDARLEVGDVSETVNVVAGAGLDLQKMDATIGSVVPGMSLLA
                     LPSLERSAVEFLTLQPGTIPEAGDGDMGNRGGAVTGARTDQSTFTLDGIDITENSTGG
                     GAGFRTMIPVPVESVEEFRVGVTNLNASFARSSGGQVSLVGRRGSNEFHGAAYWYHQN
                     DNLNANSWTNNRSGIPEAESKDNRYGFRLGGPLSHNRAFFFVNYEGRNFPRAFDVTRI
                     VPTDSLRRGIIRMRDNAGRIVSYDLATAALCSPTGNRQCDPRRLGLSPSVSALWSLLP
                     QGNDPASGDGLNTTGWRSTAVAPLENDYVVSRFDYSLTPVWSFNASATYARRLSTLGG
                     ASGLRQQLDIRDSSRAVFTGDAPLRGQNLTFGLNGSLTPAFNSSFKFGWTRDRQASDN
                     INPSRVANLLAIPGTDTGAGRVALNIGGGNLDQLVSEPIDIDSQRARSQSNDNESFQF
                     IYDGAWTKGSHSMQFGSNARHIITRHVRNDKVAGSLASLVADVFRGSNILIPNTSRPP
                     TCSTPNQANCLRANDVAQWDALFAATTGMVDTISVLTVRDADLNPLPFGTPLSADTTH
                     NAYDFYLQDTWQARPTLALTLGLSYGWQRPPHDKSGRQTRMINVANGEPLTYANYIEP
                     RREAALRGEIYNPRIG"
     gene            complement(2520..2963)
                     /locus_tag="VGB76_05855"
     CDS             complement(2520..2963)
                     /locus_tag="VGB76_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0098460.1"
                     /translation="MVANPPEDMPRIVPHLFYDDVAAAIDWLVEAFDFKVRVRMTDPQ
                     GGVVHGELECADSLVMLGLAAENAAWESPRSLDGRISQRLYIYVDDVDAHHARARDAG
                     AKVLYEPGDQFYGDRVYECIDPEGHRWKFAQHIRDVDMKTLQRPD"
     gene            3392..4567
                     /locus_tag="VGB76_05860"
     CDS             3392..4567
                     /locus_tag="VGB76_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098461.1"
                     /translation="MAYKRRRFNLRIWGLALGYFGFYAPYSALVKLTSTGMLASTNGA
                     VSGFEILPSFIVGVVIVMLVGTTLAGWGKYARRRQCFGVSVVWPGRLVLLSGLGTAII
                     IATTTLALALTGVSILFAALLMRGGVLVLAPTVDALFGRRVRWFSWCALALSFAAVLV
                     ALADVNNYRMTLVAALTIAAYLFGYLLRLPCLNKLGKCEDRSVCYRYLVEEQFVAAVL
                     LLAIPALCAFAGQDGIMLELRSGFTNFFTSGVTAPGLLIGALYACLYFFGTLIYLDAR
                     ENSFCIPLNRASSLLAVVFATYVLADLFNLPSPSMAQLGSSILIIAALMFLSPLHHGH
                     RVANQVRHAFGVAYRAFVADFGKQNAAAAPLAQTAAPEHMAPGVNEPLTEEDRFTAN"
     gene            4648..5889
                     /locus_tag="VGB76_05865"
     CDS             4648..5889
                     /locus_tag="VGB76_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HEY0098462.1"
                     /translation="MNDPIRVLICGTGAGAHVLAGILSMQTETDVRVFTQNTEKIHRW
                     RESMWSEPLTVNMREGNVERVALKARPFVVTSDPRLARGCDFIIFAVPAFMHSRYLAL
                     LQPYIEDGCVIVGLPGQGGFEFDVREALDERQQNCIVMNFESLPWICRLVEFGKAVSI
                     FGTKDSLVGALQGDLFKARVADPLAFIQQLLGETPKLVVSGHLLGITLRSPNGCAHPP
                     IMYGRWKDWDGETLEHEPLFYQGIDESTAELLSSVSEEVVTLSRRIMAAHYPLDLSQV
                     IPMYDWDISCYGKDISDKTNLMTALRTNSGYKGITHPMVRTAEGAYLPDFNHRFLAED
                     VPYGLVVIRGIAEIARVPTPCIDTVLRWCQQRLGREYLVGSRLIGKDLATTRCPQRYG
                     LKTISEVLGFDWVSAQPAQVR"
     gene            complement(5861..>6355)
                     /locus_tag="VGB76_05870"
     CDS             complement(5861..>6355)
                     /locus_tag="VGB76_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY0098463.1"
                     /translation="SDATSGHKFHHGTSVRSVIEEGLVRGDRIFQIGIRGSIASAEYQ
                     DYAKGAGIRALDMQNFHDAVRRTEFLETLREVAGSNPCYLTFDIDGIDPAFAPGTGTP
                     AVGGLTSFEALNTMRALKGLRFIGGDVVEVAPAYDSSEITSLLAATIAFELAALIALA
                     RAAR"
BASE COUNT         1127 a         1894 c         2030 g         1304 t
ORIGIN      
        1 ccgatgcgcg gattgtaaat ctcgccccgg agcgcggcct cgcggcgcgg ttcgatgtag
       61 ttggcgtagg tgagcggttc gccgtttgcg acattgatca tgcgcgtctg ccgcccggac
      121 ttgtcatggg ggggacgctg ccagccgtag gagaggccga gcgtgagcgc gagcgtgggg
      181 cgcgcttgcc atgtgtcttg caggtagaag tcgtaagcgt tatgggtcgt gtcggcggag
      241 aggggcgtgc cgaagggcag cggattcagg tcggcatcac ggacggtcag gacgctgatg
      301 gtatcgacca tgcctgtcgt cgccgcgaag agcgcgtccc attgggcgac atcgttggcg
      361 cggaggcagt tggcctggtt gggcgtcgag caggtgggcg ggcgcgaggt gttggggatc
      421 agaatgttgc tgccgcgaaa gacatccgcg acgagcgagg cgagcgagcc tgcgacctta
      481 tcgttgcgca cgtggcgcgt gatgatgtgg cgcgcgttcg agccgaactg catcgagtgg
      541 ctgcccttcg tccatgcgcc gtcgtagatg aattggaaag attcgttgtc gttgctctgc
      601 gagcgtgcgc gctggctgtc gatgtcaatc ggctcgctga cgagttggtc gagattgccg
      661 ccgccgatgt tgagcgcgac gcgacccgcg cccgtgtccg tgccgggaat cgccagcagg
      721 ttcgccacgc gcgaaggatt gatgttgtcg ctcgcctgcc tgtctctggt ccagccgaac
      781 ttgaagctgc tgttgaaggc gggcgtgagc gagccgttga ggccgaacgt gaggttctgc
      841 ccgcgcagcg gcgcgtcgcc ggtgaagacg gcgcggctgc tatcgcgtat gtcgagttgc
      901 tggcgcaggc cgctcgcgcc gccgagcgtg gagagacggc gggcgtaggt ggccgaagcg
      961 ttgaaagacc agacgggcgt gaggctgtag tcgaagcgcg acacgacgta gtcgttctcc
     1021 aaaggcgcga cggcggttga acgccacccc gtggtgttca gtccgtcgcc gctcgccggg
     1081 tcgttgccct gcggcagcag cgaccagagc gcggagacgc tcgggctgag tccgaggcgg
     1141 cgcgggtcgc actggcggtt gcccgtcggc gagcagaggg cggcggtcgc tagatcgtaa
     1201 gagacgatgc gcccggcgtt gtcgcgcatg cggatgatac cgcgacgcag actgtctgtg
     1261 ggcacgatgc gcgttacgtc gaaggcgcgc gggaaattgc gcccctcgta gttcacgaaa
     1321 aagaaggcgc gattgtggct gaggggacca ccgagacgga agccgtagcg gttgtccttc
     1381 gactccgctt cggggatgcc gctgcggttg ttagtccaac tgttggcgtt gagattgtcg
     1441 ttttgatgat accagtaggc cgcgccgtga aactcgttcg agccgcgccg cccgacgagc
     1501 gacacctgcc cgcccgacga gcgcgcgaaa gaggcgttca gattcgtcac gccgacgcga
     1561 aactcttcca cgctttcgac cggcacgggg atcatcgtgc ggaagcccgc gccgccgccc
     1621 gtggagttct ccgtgatgtc tatgccgtcg agcgtgaagg tgctctggtc tgtgcgtgct
     1681 ccggttaccg cgccgccgcg attgcccata tcgccgtcgc ccgcttcggg aatcgtgccc
     1741 ggctggaggg tgagaaactc gaccgcgctc cgttccagcg agggcagggc gagaagcgac
     1801 atgcccggca cgacgctccc gatagtcgcg tccatcttct gcaaatcaag tccggcgccc
     1861 gcgacgacgt tgaccgtctc cgacacgtcg ccgacctcaa gcctcgcgtc gaagctataa
     1921 gacttcgcca cctcgatctt caaatcggaa aaggacgcct gccggaagcc ctgcatgcgt
     1981 acgacgagcg cgtactcgcc gggcagcaca ggcgagaaga cgtaatgcgc cagactgttg
     2041 ctctgttgct tctggacgag tccggtcgct acgtttcgca actctacttc cgccccggcc
     2101 aagccttcgc cgttgctgtc aattattctg cccgagacgg tgctggtcga agaggtctgc
     2161 gcgcgagcga ttgcctgcgc gttcaggatc aggagggcgg cgcacaggaa gaacattatt
     2221 cgccgcgcgg aattagggaa gcgcgtgagt ccggttgaga gtttcataga gagtaggtat
     2281 agggggttca agttggcaac aactatctgg aagcgcacgc ggagagttgg ttaatggctg
     2341 cttccgcttg ccttaaattt ttcccgccgc gctcgttgct cagagagagc gttttataac
     2401 tatcgctctc ttttccctct ggcctccggc gcgagactcg atctttgcga aagaagataa
     2461 cttgcgccgg gacgcgggag cgtgaaggag cattgagaga ggaaggttga aaggcactct
     2521 taatcggggc gttggagggt cttcatgtcc acgtcccgta tgtgttgggc aaacttccag
     2581 cgatggcctt cggggtcgat acactcgtag acgcggtcgc cgtaaaattg gtcgccgggt
     2641 tcgtacagca ctttcgcgcc cgcgtcgcgc gcacgcgcat gatgcgcgtc cacgtcgtcc
     2701 acgtagatgt agagccgttg actgatgcgg ccatcgagcg agcgcggact ctcccacgcc
     2761 gcgttctcgg cggcgagtcc gagcatcacg aggctatccg cacattcgag ttcgccgtgg
     2821 actacgccgc cctgcgggtc ggtcatgcgc acgcgcacct tgaagtcgaa ggcttcaacc
     2881 agccagtcaa tcgcggcggc gacatcatcg tagaacaggt gcgggacgat gcgcggcatg
     2941 tcctccgggg gattggcaac catctctgca tgctccttgc gtattcactt acagcccttg
     3001 caacgaccga cgctactgta tagcgaaatc attttggatt gtctagcttc ttctgtttcc
     3061 gtggccgcca gcacggcgcg ggcgcgcgac gggcgtgcgt gtcgcttatc tgcaagcctg
     3121 ccgcgcgatg agtgtggcgc attgcagccg cgagaaaaat gttgattttt ttgcctgact
     3181 tgtcatattg tcgaaccatt ctcaccaggg cgggtctggc aagcgcacta caggaaaata
     3241 gcgacgacaa tctgccgaca gagatttgta agtcggttcc gaagctttac tcacggcctt
     3301 atctttatct ttctgcaaac aatttaagaa cctcgaaact tcccggttat cttcagtcaa
     3361 cgctcccgtt catttaagac cggagggtca aatggcttac aagcggcgac ggttcaattt
     3421 aaggatctgg ggtctggcct taggctattt cggtttctat gcgccctaca gcgcgctcgt
     3481 caaactcacc tcgacgggca tgctggcgag cactaacggc gcagtcagcg gctttgaaat
     3541 cctgccctcg tttatcgtcg gcgtcgtgat cgtgatgctc gtcgggacga cgcttgccgg
     3601 ttgggggaag tatgcgcgtc gccgtcaatg cttcggcgtc agcgtcgtct ggccggggcg
     3661 tctggtgctt ctctccgggt tgggcacggc catcatcatc gccacgacca cgctggcctt
     3721 agccctgacc ggagtttcca tcctcttcgc ggccttgctg atgcgcggcg gcgtccttgt
     3781 cctcgcgccc acggttgacg cgcttttcgg gcgcagggtg cgctggttct cctggtgcgc
     3841 gctcgcgctc agtttcgccg ccgtgctcgt cgctctggcc gacgtgaata actaccggat
     3901 gaccctcgtg gccgcgttga ccattgcggc ctacctgttc ggttatttgc ttcgcctgcc
     3961 ctgcctgaac aagttgggga agtgtgaaga cagaagcgtc tgctatcgct acctcgtgga
     4021 agagcaattc gttgcggcgg tgctgctgct ggctatcccc gccctctgcg ccttcgccgg
     4081 gcaggacggc atcatgctcg aactgcggag cggcttcacg aacttcttca cgagcggcgt
     4141 cactgcgccg ggtcttttga tcggcgcgct ctatgcctgc ctctacttct tcggcacgct
     4201 catttatctg gacgccaggg aaaactcttt ctgtattccg ctcaatcgcg cttcgagttt
     4261 gctggccgtc gtcttcgcca cctacgtcct cgcagacctc ttcaacctgc cgtcgccaag
     4321 catggctcag cttggcagtt caatcttgat catcgccgcc ctgatgtttc tctcgccgct
     4381 gcatcacggg catcgcgtcg ccaatcaagt gcgccatgcg ttcggcgtcg cctatcgcgc
     4441 gttcgtcgca gacttcggca agcaaaacgc cgccgctgcg cctctcgcac agaccgccgc
     4501 gccggagcac atggctcccg gcgtcaacga gcctctgacc gaagaagacc gcttcactgc
     4561 gaattaaccc ggaagtgacc gggaagtggc cgggacgtga cggggaagca atggagaagc
     4621 aactggccgg acggggaaac tctcactatg aatgatccaa ttcgagttct catctgcggc
     4681 acgggcgcgg gcgcgcacgt tttggcgggc atcctctcga tgcaaacgga gacggacgtt
     4741 cgcgtcttca cgcagaacac ggagaagatt caccgttggc gcgagagcat gtggagcgag
     4801 cctctcaccg tcaacatgcg ggagggaaac gtcgagcggg tggcgttgaa ggcgcggcct
     4861 ttcgtcgtca cgagcgatcc gaggctcgcg cgcgggtgcg acttcatcat cttcgccgtg
     4921 cccgccttca tgcactcacg ctatctcgcc ctcctgcaac cctacatcga ggacggttgc
     4981 gtgatcgtcg gcctccccgg tcagggcggt ttcgagttcg atgtgcgcga ggctctggac
     5041 gaaagacagc agaactgcat cgtgatgaat ttcgagtcgt tgccgtggat ttgtcgtctc
     5101 gtggagttcg gcaaagccgt gagcattttc ggcacgaaag atagtctggt cggcgcgctg
     5161 caaggcgacc tcttcaaggc gagagtggca gacccgctcg ccttcatcca gcagcttctg
     5221 ggagagacgc cgaaactcgt cgtctccggc cacctgctcg gcatcaccct gagatcgccg
     5281 aacggctgcg cacacccgcc gatcatgtac gggcgttgga aagattggga tggcgagacg
     5341 cttgagcatg agccgctctt ttatcagggc atagacgaga gcacggcgga actgctctcc
     5401 agcgtgagcg aggaggtcgt cacgctgagc aggcggatca tggcggcgca ctatccgctg
     5461 gacttatcgc aggtcatccc gatgtacgac tgggacatct cctgttatgg caaagacatc
     5521 tcggacaaga ccaacctgat gaccgcgctg cgcaccaact ccggttataa gggcatcacg
     5581 catcccatgg tccgcacggc ggagggcgcg tacctgccgg acttcaatca ccgctttctg
     5641 gccgaagacg ttccctacgg gctggtcgtc attcgcggca tcgcggagat cgcgcgcgtc
     5701 ccgactccct gcatcgatac ggtcttgcgc tggtgccagc aacggctggg gagagagtat
     5761 ctggtcggct cacgcttgat cggcaaagac ctcgcgacga cgcgctgccc gcagcgatac
     5821 ggactcaaga cgatctcgga ggtgctcggt ttcgattggg tcagcgcgca gcccgcgcaa
     5881 gtgcgataag cgcggccaac tcgaaggcga tggtcgccgc gagcagcgaa gtgatctccg
     5941 aggaatcgta agcgggcgca acctcaacca cgtcgccgcc gataaatcgc agccccttga
     6001 gcgcgcgcat cgtgttcaat gcttcaaaac tcgtgagtcc gccgacggcg ggagtgcccg
     6061 tccccggagc gaacgccgga tcaataccgt caatgtcgaa cgtgagataa cagggatttg
     6121 aacctgctac ttcgcggagc gtttcgagaa actccgtgcg ccgcacggca tcgtgaaaat
     6181 tctgcatgtc gagcgcgcgg atgcccgctc ccttcgcata gtcctgatat tccgcgctcg
     6241 caatcgagcc gcgaatgcct atctgaaaga tgcgatctcc gcgcacgagt ccctcttcga
     6301 tcacgctgcg gacggatgtg ccgtgatgaa atttgtgccc ggaggttgca tcgga
//
LOCUS       DASWNS010000169         4732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_85218, whole genome shotgun sequence.
ACCESSION   DASWNS010000169 DASWNS010000000
VERSION     DASWNS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85218"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VGB76_05875"
     CDS             complement(<1..494)
                     /locus_tag="VGB76_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098464.1"
                     /translation="MIYYIFYELIFQRYGAGEPSYFIKALNVFQYVTFRTAYATVTAL
                     LISLVFGGRVIRMLREMNVGQQIREEGPQAHMAKRGTPTMGGVLIIGSVLISTLLWAR
                     LSNLYVWIILISTALFAVIGFLDDYAKVAKRQSLGLTGKQKLAAQFLIALGVWATLLL
                     FAGDT"
     gene            complement(581..2074)
                     /gene="murF"
                     /locus_tag="VGB76_05880"
     CDS             complement(581..2074)
                     /gene="murF"
                     /locus_tag="VGB76_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY0098465.1"
                     /translation="MKIRTASQWMRAERADTGETREFFDKELTGFSIDSRTVAPGELF
                     FALSPADYRRHFFTATSFDDAHRFIPVAFERGAAAVVARRAGVAGHPELEAYGERLLF
                     VEDCIEALQRVAHGVLEAWGGTVVALTGSAGKTTAKDLTAHVLAQTGRRVLKTQKNFN
                     NELGLALSILQMETRGARPEDFDVAVLEMGQSMFGEIARLAQVAPPDIGVELNVAPVH
                     LEFFDSVEHIAAGKAQLIENLKPEGTAILNADDARVAAMRSKHDGRTLTFGIENPADV
                     SAVEIESARFGLSRFRLVTPMGAAQAELPLPGRHNLLNALAAAAVANCFRVAPEEIAQ
                     ALSSASASEMRGEVLEFASGFSVIDDSYNSNPRSLLSMARALAEAKGAPGGGEEKARR
                     TFVVAGEMLELGVLAPEMHREAGREIAQMGIDVLWGVRGLASELVAGAREAGMEAARF
                     FETTEEAAALLSEEARTGDLLLVKGSRGVHMERIIELLRERYEGSVE"
     gene            complement(2330..4453)
                     /locus_tag="VGB76_05885"
     CDS             complement(2330..4453)
                     /locus_tag="VGB76_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEY0098466.1"
                     /translation="MMPERSNAKTKNRPDASRQRVSFIVAILVVWMLVIGVRLVQLQV
                     GQYEDLSRRAQKQQQHAERTNALRGLILDRNGRELARSLDVDSFVAVGTDAKFEPREA
                     AKKLASVLGVAESPLRARLEEAKRAKREFVWIARQVDPEQAAKIHELKLAGVRVVKEP
                     KRYYPNGALAAHVLGYVGTDDNAQSGIELFQNAQLRGEEGKTVFDSDGRHRSFNSFEI
                     EAQPGQSVVLTIDQNIQFQVERAVATAMEQTRAKAATALVLDPRTGEILALANAPTFD
                     PNHAKKIAADVRKNQALQNIYEPGSTFKIVAYSAAIEEKLVTPTDPIDCQMGSINLFG
                     RIIRDTHAYGTLSITEALAKSSNVAAIKLGMRVGKERMYDYMMRYGFNQKTGIELAGE
                     TRGVVRRPEKWHPSSMGSVAIGQEVGVTPIQMAAAFAAIANDGVRVAPHLVREVQDAN
                     GQVIQRTTPEAHRVVSVETARTMRPMLESVTLDGTAKQAQLDGYTAAGKTGTAQKIDP
                     ATRAYSKTKYVASFVGFAPVENPAVVIIVVIDEPAGSYYGGTVAAPVFRQIAENVLPY
                     LDVAPDTEFKTPTDAEQNLAQGFAPTEAEGTLHHAAQHSGTAPAAQSTQPTTPDAMLP
                     QVAERRRGGAGGVGEVVYAAASERALLMPDIRGRSVREAQRICAQLGLELEAHGDGRA
                     VGQSPAAGAQIVAGQTVRVEFGRSD"
BASE COUNT          814 a         1589 c         1408 g          921 t
ORIGIN      
        1 gtatcgcccg cgaataagag gagcgtcgcc cacacgccga gcgcgatcaa aaactgcgcg
       61 gccagtttct gcttgccggt caagcccaga ctctgccgct tagccacctt cgcgtagtcg
      121 tccagaaagc cgatgacggc gaagagcgcg gtcgagatca ggatgatcca gacgtagagg
      181 tttgagagac gcgcccacag cagcgtggag atgagcaccg agccgatgat gaggacgccg
      241 cccatcgtcg gcgtgccgcg cttcgccatg tgcgcctgcg gcccctcctc cctgatctgc
      301 tgcccgacgt tcatctcacg cagcatgcgt atgacgcgac cgccgaagac gagcgagatg
      361 aggagcgcgg taacggtggc ataggccgtg cggaacgtga cgtattgaaa gacgttgagc
      421 gccttgatga aatacgacgg ctcgcccgcg ccgtaacgct ggaagatcaa ctcgtagaag
      481 atgtaataga tcatcctttt tgagtgatga gtgatgagtg atgagcgatg agttaaagac
      541 aaaggcaagg ctgttgtttt aactcatcac tcatcactta tcactcaaca cttccctcat
      601 atcgctcgcg tagcagttcg atgatccgct ccatgtgcac gccgcgcgat cctttcacca
      661 gtaacagatc gcccgttcgc gcctcttccg acaaaagtgc ggcggcctct tccgtcgtct
      721 caaaaaacct cgccgcctcc atccccgcct cgcgtgcgcc cgcgacgagt tcgctcgcca
      781 gtccacgcac gccccacaaa acgtcgatcc ccatctgcgc gatctcgcgt cccgcctcgc
      841 ggtgcatctc cggcgcgagc acgcccaact ccaacatctc gcccgcgacg acgaacgtgc
      901 gtcgcgcctt ctcttcccca ccgcccggcg cgcctttcgc ttccgccagc gcacgcgcca
      961 tgctcaagag cgagcgcggg ttcgagttgt aggaatcgtc tatcacgctg aaccccgacg
     1021 cgaactccaa cacctcgccg cgcatctccg aagccgaagc cgacgacaga gcttgcgcga
     1081 tctcttccgg cgcgacgcga aaacagttgg cgaccgccgc cgccgcgagc gcgttcaaga
     1141 ggttatgccg accgggcaac ggcaactccg cctgcgccgc gcccatcggc gtgacgagac
     1201 gaaagcgact caggccgaac cgcgcgcttt caatctccac tgcggacacg tccgccgggt
     1261 tctcgattcc gaaggtgagc gtgcgcccgt cgtgcttgct ccgcatcgcc gcgacgcgcg
     1321 catcgtctgc atttagaatc gcagtgcctt caggtttcag attttcaatg agctgtgcct
     1381 tgcccgccgc gatgtgctcg accgaatcga agaactcaag atgcacgggc gcgacgttca
     1441 actcgacgcc gatgtccggc ggcgcaacct gcgcgagccg cgcgatctct ccgaacatcg
     1501 actgccccat ctccaagacc gccacgtcga aatcctccgg gcgcgccccc cgcgtctcca
     1561 tctgcaagat cgagagcgcg aggccgagtt cgttgttgaa gttcttctgc gtcttcaaca
     1621 cgcgccgtcc cgtctgagcg aggacgtgcg ccgttagatc cttcgccgtc gtcttccccg
     1681 cgctccccgt gagcgcgacg accgtgccgc cccaggcctc aagcacgccg tgcgccacgc
     1741 gctgcaaagc ctctatgcaa tcttcgacga acaggagacg ctcgccgtag gcttcgagtt
     1801 ccgggtggcc tgcgacgccc gcgcgccgtg ccacgaccgc cgccgcgccg cgctcgaagg
     1861 cgacggggat gaagcgatgc gcatcgtcga atgaagtggc ggtgaagaag tgccgccggt
     1921 aatccgcggg cgagagggcg aagaaaagtt cgcccggcgc gacggtgcgc gagtcaatcg
     1981 aaaaccccgt caactctttg tcaaagaact ctcgcgtctc ccccgtatcc gccctttcgg
     2041 cacgcatcca ttgagacgcc gttcgtatct tcacgctgct attttttcct ttgcccttct
     2101 tttttctgcg tctcgggcgg cttcgcgggc gcgccctcgt ggctcgattt ctgcgcgctg
     2161 cgcctgcgcc tgagacgctc ccggcaatct acgattctac gtctcagttc atattgctga
     2221 ctatcttgcg ggggaactgt cattgagtcg ctccgttacc gatttctttg ttctccccgg
     2281 cgcggcgggg acgggcacga cacgaacggc gaacactatt tctcgcccct cagtcgcttc
     2341 tgccgaattc gacgcggact gtctgaccgg cgacgatctg cgcgcccgcc gcggggcttt
     2401 gcccgacggc gcgaccgtcg ccgtgagctt cgagttcgag tccgagttgg gcgcagatgc
     2461 gctgcgcctc acgcacgctg cgtccgcgta tatccggcat caacagcgcg cgctccgacg
     2521 ccgccgcata gaccacttcg cccacgccgc ctgcgccgcc gcgccgtcgc tccgccacct
     2581 gcggcaacat cgcgtccggc gtcgttggtt gagttgattg cgcggctgga gcagtcccgg
     2641 aatgttgcgc cgcgtggtgc agagtccctt cggcttcggt cggggcaaat ccctgcgcga
     2701 gattctgctc cgcgtccgtg ggagttttga attccgtgtc gggcgcgaca tcgagataag
     2761 gcagcacgtt ctcggcgatc tggcggaaga cgggcgcggc caccgtgccc ccgtaataag
     2821 aaccggcagg ctcgtctatg acgacgatga tcacgaccgc aggattctcc acgggcgcga
     2881 agccgacgaa cgaggcgacg tatttcgtct tcgaataagc gcgcgtcgcc gggtcgatct
     2941 tctgcgcggt gccggtcttg cccgccgccg tgtagccatc gagttgcgcc tgcttggccg
     3001 tgccgtccaa agtcacgctc tcaagcatcg gcctcatcgt gcgcgccgtc tccacgctca
     3061 cgacgcgatg cgcctccggc gtcgtgcgct gaatgacctg cccgttcgcg tcctgcactt
     3121 ctctgacgag atgcggggcg acgcgcacgc cgtcgttggc aatcgcggcg aaggccgccg
     3181 ccatctggat cggcgtcacg ccgacctcct gcccgatggc gaccgaaccc atcgaactcg
     3241 gatgccactt ctcaggccgc cgcacgacgc cgcgcgtctc gcccgccagt tcgatgccgg
     3301 tcttctggtt gaagccgtag cgcatcatgt agtcgtacat gcgctctttg ccgacacgca
     3361 tccccaactt gatcgcggcc acgttgctcg actttgccaa cgcttccgtg atgctgagcg
     3421 tgccgtaggc gtgcgtgtcg cggatgatgc gaccgaagag attgatcgaa cccatctggc
     3481 aatcaatcgg gtcggtcggc gtcacgagtt tctcttcgat ggcggcggaa taggcgacga
     3541 tcttgaaagt cgaacccggc tcgtaaatat tctggagcgc ctgatttttg cgcacgtcgg
     3601 cggcgatctt cttggcgtgg ttcgggtcga acgtgggggc gttggcgagc gcgagaatct
     3661 cgcccgtgcg cgggtcgagc acgagcgcgg tggccgcctt cgcgcgcgtc tgttccatcg
     3721 ccgtcgctac cgcacgctca acttgaaatt gaatgttttg atcaatcgtc agcacgacgg
     3781 actggccggg ctgcgcctcg atctcgaaac tgttgaagga acggtgtcgc ccgtcgctgt
     3841 cgaaaacggt cttgccctct tcgccgcgaa gttgtgcgtt ctgaaaaagc tctatgcccg
     3901 actgagcatt gtcgtccgtg ccgacgtagc cgagcacgtg cgccgcgagc gcgccgttcg
     3961 ggtaataacg cttcggctct ttcaccacgc gcacgcccgc gagtttcaac tcgtgaattt
     4021 tcgccgcctg ctccggatca acctgtcgcg cgatccacac aaactcccgc ttcgcacgct
     4081 tcgcttcttc cagacgcgca cgcagcggcg actccgcgac gccgagcacg gaagccaact
     4141 ttttcgccgc ctcacgcggc tcgaactttg catccgtccc cactgcgacg aacgaatcga
     4201 cgtcgaggct gcgcgcgagt tcgcgcccgt tgcggtcgag gatgaggccg cgcagggcgt
     4261 tcgtccgttc tgcgtgttgc tgctgcttct gcgcgcggcg gctcaagtct tcatactgcc
     4321 cgacctgtag ctggacgagc cgcacgccga tgacgagcat ccacacgacg aggatagcca
     4381 cgatgaaaga gacgcgctgg cgcgacgcgt caggccggtt ctttgttttt gcgttagagc
     4441 gttcgggcat catggcagac aactctcttc aaacgattcg taccgcgcgc cagccaacct
     4501 cggtcaacag cgggagccga aaaaacctgc gcgcggggaa ctcggagcca aagcgtgggg
     4561 aaccagcgtt aaggcgaggg gaacgaagtc gaagcgttcg ggtcatgtgc gcttctctct
     4621 ggtcaatgcg cgacggaacg cgcgcggggc tgcttcttgg cggtcggcgg ggaggccgaa
     4681 gtcttctgcg tgatactcgt cgtcgctgtc gtcgctgtcg tcgctgtcgt cg
//
LOCUS       DASWNS010000170         8630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_8158, whole genome shotgun sequence.
ACCESSION   DASWNS010000170 DASWNS010000000
VERSION     DASWNS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8158"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..3224
                     /locus_tag="VGB76_05890"
     CDS             369..3224
                     /locus_tag="VGB76_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY0098467.1"
                     /translation="MTRKRISLDGSWEFLPDPNQSLQPQTFTEAGTPRSIRVPGPWQA
                     QFEDLRGYTGVAWYRRQFEIPQIEEIRAHSDTTYILHFGAVDYFASVWLNGQLVGEHE
                     GGYLPFEFALDGALRLAGVNELVVRVVDPGDHEQLSPDISFAEVPHGKQSWYGPIGGI
                     WQSVYLEARHTTYITSLHITPDVERAQAHVRLRLNRRASHAFPLRLTLTDPRGAVTVH
                     NLIIDDGADSFQTLLPVPDPLLWETHRPHLYHLEAALLAAESEGAVLDALASHFGMRS
                     IAASPDGHLMLNGRILYLRGALDQDYYPEMIYTPFSDAELDEQFVKAKHMGLNCIRTH
                     IKISDPRYYNAADRAGMLIWTELPNWENLTPATKERARATLDGMVERDWNHPSIIIWT
                     IVNEGWGVELAVNAEHRAWLSETYDHLKRLDPHRLVVGNSACFTNFHVVTDIEDFHNY
                     YSIPDHYRQWRAWVETFAARPPWTFAHTYENNDAWRQFLRDPWKAVPRTPAPEVRRTG
                     REPMIVSEFGNWGLPDLEKLRACYGGRDPWWFETGLEWGDGAAYAHGVEIRYKTWHLD
                     KVFPTFTDLARASQRMQFCALKYQIEQMRRHPSIVGYIITEFTDVHWEANGLLDMCRN
                     PKAFYDAMAGINGADAIIPAWKRVAFWEGEQCEVSLTLSHFSESDLDGAQLEWHLDLF
                     PEINGVLRRVRPQKAQITKLGKVVFEVPQLDASVRARLELRLIDARGEEVTSNHQELY
                     FFPRRAAHVPPVRLSASPRLAPQLEALGYELTDDWAHADLLVADKLTDEVRFFVQNGG
                     RVLWLAESSSAQQSYLGTVEILPRQGRRWQGDWASNMNWIRQAEMFREIPTGGTVDFA
                     FADLTPEHVITGINPRDFAADVHAGLFVGWLHHIAALVVERRYGSGRLLISTFRLREQ
                     LRGNPVAAIMLRDMIAHLTKDRMANEKSERRMAMATN"
     gene            complement(3261..3449)
                     /locus_tag="VGB76_05895"
     CDS             complement(3261..3449)
                     /locus_tag="VGB76_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098468.1"
                     /translation="MTTAAKLVASAETVNDNKTLTGAQGTKSLKATASGYSPQEEAQR
                     RKWGVERGAVRSIHARKF"
     gene            complement(3708..4304)
                     /locus_tag="VGB76_05900"
     CDS             complement(3708..4304)
                     /locus_tag="VGB76_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098469.1"
                     /translation="MAFGVIMLAATPAFEGRNMQIASQAQNAVSPPAGKNFLTGTYRE
                     KNSQDAEIGRKVLSATARLDDGERERQGRALLRTLRAAPHFAIEQYGTTIMMNYPHGI
                     RVPYEADGKTRIMRATGGENVTVRATLETDRLTIDLTWSGGERLRLIFDSPAQRGNLT
                     YTRIAGNRFLERPITVTSVYERTSGKATRIFERLAATQ"
     gene            complement(4534..5256)
                     /locus_tag="VGB76_05905"
     CDS             complement(4534..5256)
                     /locus_tag="VGB76_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0098470.1"
                     /translation="MRKFFFIASILLVCVPLTFAQGTTTARTGPEIFVGYSNLQAEGI
                     PGEDTMPGGGGGGSLDDELFGQRSGLHGINVAVTGFFTPRVGLTGDFAFHKRNLNSDN
                     GQTGVGSARTELDTRVVSFLGGPTMRFPNTSRVTPFVRALFGVANTRFEAEQSRTLAT
                     GTVQNTFETSSTDFSMALGGGLDVRLNERVGLRLIQFDYNPVFLRDRSVQVLGAAGAL
                     VTQQLESQRQDNLRFSFGVTFK"
     gene            complement(5562..6389)
                     /locus_tag="VGB76_05910"
     CDS             complement(5562..6389)
                     /locus_tag="VGB76_05910"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEY0098471.1"
                     /translation="MELSEARGKLVVIGGAEDREGDCIILKEFVRLAKGDKAHIVVMT
                     VATDHPEEVGADYKKIFKRLGAADVKVVDVSQREDTESSKAMDAIEEATGLFFTGGDQ
                     LHITALLGGTEMDRIIKKRFGKGLVLGGTSAGAAMMSNSMMLGGESETNPRFGSVEIG
                     PGMDFLMGTLIDTHFSQRGRHGRLITATAHYPQDMGIGIDEDTAMVVNRNEFEVIGSG
                     AVTIIDAGGITHTNLHEIRKEEGIELHNIRVHILPRGARFSLTERKPLALEAAGQGR"
     gene            complement(6572..>8630)
                     /gene="cphA"
                     /locus_tag="VGB76_05915"
     CDS             complement(6572..>8630)
                     /gene="cphA"
                     /locus_tag="VGB76_05915"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR02068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY0098472.1"
                     /translation="IGVEIAGDKDLTKARLAKASIPVPEGEIVYTEEEAVAALNTIGA
                     PVVVKPLDGRQGKGVSLHLSTPDEIVNAFHIAREFSREVLVEEMFGGRNYRVLVVGYR
                     MVAASERIPCHVMGDGTHTVAELIEIENQNPLRGEGHEKPLTKIKKDDPILTTFMRKE
                     GWSMDAVPAAGERVMLCAGMNLSTGGTAKDVTDEVHASVRSLCERAARLMGMDVCGVD
                     LVVDDIAAPVGKGGGVIEINAAPGLRMHQHPGEGQPRDVGREIVEMLFPAGSGGRIPI
                     ISITGTNGKTTVTRMISHILIEAGLVTGTTTTDGIYLNGERIVRGDTTGPVSAMTILT
                     DKAIEAAVLETARGGIVRRGLGYDWSDISVITNVGADHIGQDGIESVEDILWIKSLVA
                     ERVREGGTLILNADDERLARLSERAAVSRVPKKYVYFSLREDNPVLLQHCDAGGTAYF
                     YRDGWLVELEGGSEHAFIEAAAIPATMNGSADFQIANALAAVAAARAFGLSREEVGAS
                     IARFRSDAENPGRANLYRVGGGYVMVDYGHNPDAFDAVCRMAARWEDRRVTGIIGVPG
                     DRDDSVVEQAGRVAARGFHRIIIKEDKDLRGREKGEVAKLLCEAVNDEWPGTECRIVL
                     DEVEALRSELQEMQEGQVVVIFYDKLGPVLDALREYGAHPVGAIEARRQPVAVSQESV
                     GVQ"
BASE COUNT         1590 a         2739 c         2539 g         1762 t
ORIGIN      
        1 ctcaaatcag actatttaaa caaagccccg acgacataat cgacattatc ggactctgga
       61 attctacctg aactatccgc gccccactcg acagataaga acgaagcgtt tcaccgcgac
      121 gggcatagtt accgtggtgg gcgatgttgg gcggaacccc tttacgtctg gcggcataat
      181 gagaatctcc ctgttggctt ttcgcgccct catacagttt ttcccacaca tcccctgcgc
      241 aaaaaactct tcacacttct gcggttgaat atgaaccgct taacgactag gatcagacct
      301 gctataaact tcaaattgtt gttgatccga tcttcgcctt cagacggcgt gtatcaagca
      361 ggggatatat gactcgcaaa cgaataagtc tcgatggttc ctgggaattt ttgcctgatc
      421 cgaatcaatc cttacaaccg cagacgttca cagaggccgg gacgccgcgc agcattcgcg
      481 tgccgggacc gtggcaggca cagtttgaag atttgcgcgg ttataccggc gtcgcctggt
      541 atcgccgcca gttcgagatc ccgcaaatcg aagagatccg cgcccactcc gataccacct
      601 acatactcca cttcggcgcg gttgactact tcgcctccgt ctggctcaac ggtcaactcg
      661 tcggcgaaca cgaaggcggc tacctgcctt tcgagttcgc tttggatggt gccctgcgcc
      721 tcgcaggcgt caacgagttg gtggtgcgcg tcgtcgatcc gggcgaccac gagcagcttt
      781 cgcccgacat ctcttttgcc gaggtgccgc acggcaagca gagttggtac ggaccaatcg
      841 gcggcatctg gcagagcgtc tatctggaag cgcgacacac cacttacatc acgagcctgc
      901 atatcacgcc cgatgtcgag cgcgcgcagg cgcacgtccg cctcaggctc aaccggcgcg
      961 cgagccacgc cttcccgctg cggctgacgc tcaccgaccc acgcggcgcg gtgacggttc
     1021 acaatctaat catcgacgac ggagcagact ctttccagac actcctgccc gtccccgatc
     1081 cgctcctctg ggagacgcat cgcccgcacc tatatcatct cgaagccgcg ctgctagccg
     1141 ctgaaagcga aggcgcggtg ctcgatgcgc tggcctctca tttcggcatg cgctccatcg
     1201 ccgcttcacc cgacggccat ctcatgctca acgggcgcat cctctacctg cgcggcgcgc
     1261 tcgatcagga ctactacccg gagatgatct acactccgtt ttcggatgcg gaactcgacg
     1321 agcagttcgt caaagccaag cacatgggat tgaactgcat ccgcacgcac atcaagatct
     1381 cagacccgcg ctactacaac gccgccgacc gcgccgggat gctcatctgg acggaactgc
     1441 cgaactggga gaacctcacc ccagccacta aagagcgcgc ccgcgccact ctcgacggca
     1501 tggtggagcg cgactggaat catccttcga tcatcatctg gactatcgtt aacgaaggct
     1561 ggggcgtcga gttggccgtt aacgccgagc atcgcgcgtg gctctcggag acttacgatc
     1621 acctcaagcg actcgacccg caccgcctcg tcgtcggcaa ctccgcctgc ttcacgaatt
     1681 ttcacgtcgt caccgacatc gaagattttc acaactacta ttcgattcca gaccattacc
     1741 ggcaatggcg cgcgtgggtg gagactttcg ccgcccgccc gccgtggacc ttcgcgcaca
     1801 cctacgaaaa caacgatgca tggcgacagt ttttgcgcga cccgtggaag gccgtcccgc
     1861 gcacgcccgc gcccgaagtc aggcgcacgg ggcgcgagcc gatgatcgtc tcggagttcg
     1921 gcaactgggg cttgcccgat ctggagaaac tgcgcgcctg ttacggcggg cgcgatccat
     1981 ggtggttcga gacggggctt gagtggggcg acggcgcggc ctacgcacac ggcgtcgaaa
     2041 ttcgttacaa gacctggcat ctggataaag tctttccgac gtttacagac ctcgcacgcg
     2101 cctcgcagcg gatgcagttc tgcgccctga aataccagat cgagcagatg cgccgtcatc
     2161 cctcaatcgt cggctacatc atcacggagt ttactgacgt gcactgggaa gcgaacgggc
     2221 tgctcgacat gtgccgcaac ccaaaagcct tctatgacgc gatggcgggg atcaacggtg
     2281 ccgacgcgat catcccggcg tggaagcgcg tcgccttttg ggagggcgag caatgtgaag
     2341 tgagcctcac gctctcgcac ttctccgagt ccgatctcga cggcgcgcag ctcgaatggc
     2401 atctcgacct gttccccgaa atcaacggcg tgctgagaag agtgcgaccg caaaaagcgc
     2461 agatcacgaa gttgggcaag gtcgtcttcg aagtgccgca gttggatgcg agcgtgcgtg
     2521 cgcggctcga actacgtttg attgacgcgc gcggcgagga ggttacgagc aatcatcagg
     2581 agctatattt cttcccgcgt cgtgcggcgc acgttccgcc cgtgcgcctc agcgcatcgc
     2641 cgcgtctggc cccgcaactc gaagccctcg gttacgaatt gacagacgat tgggcgcacg
     2701 ccgaccttct ggtcgcggat aagctgacgg acgaggtgcg tttctttgtg cagaacggcg
     2761 ggcgcgtgct gtggctggcc gagtcgagca gcgcacaaca gagctatctg ggaacggtcg
     2821 agatcctccc gcgtcagggg cgacgctggc agggcgattg ggcgagcaac atgaactgga
     2881 ttcggcaggc cgagatgttc cgcgaaattc ctaccggagg tacggttgac ttcgccttcg
     2941 ccgacctgac gcccgaacac gtcatcacgg gcatcaaccc gcgcgacttc gccgcagacg
     3001 tgcacgccgg tctgttcgtc ggctggctgc accacatcgc cgcgctcgtc gtcgaacgac
     3061 gctacggcag cgggcgtctg ctgatctcaa ccttccgcct gcgcgagcaa ctgcgcggaa
     3121 atcccgtggc tgcgatcatg cttcgagaca tgatcgctca cctgacgaaa gaccgtatgg
     3181 cgaatgaaaa gagtgaaaga cggatggcga tggcgacgaa ctgagcgggt ggggctgaac
     3241 gctggagatg agtccggcgt tcagaatttt cgcgcgtgaa ttgatctgac cgcgcccctc
     3301 tcgacgcccc acttacggcg ttgcgcttct tcctgcggag aatagccgct cgccgtcgct
     3361 ttcaggctct tcgtgccctg cgcgccggtc agcgtcttat tgtcattaac cgtctccgcc
     3421 gaggcgacga gttttgcggc ggttgtcatc ttccacctcc acaccatttt cgccgttcct
     3481 gcatacatcc cttaaacaag cccggcgcgc ggttcttttg cgaacgagat ttaacagccc
     3541 tttacacgta ctggttcgaa aactgccaga ttgagagatt atagagatcg tgaggcgaaa
     3601 tttccgtacg ataacgaacg attaataagg agagttgccg aacgattaag gagagagtcg
     3661 ctgttcccga tgcctgcgcg gcgcagagaa ctgtgcggac gtgagaatta ttgcgtggct
     3721 gccagacgtt cgaagattct cgtcgccttg ccggacgtgc gctcatacac ggaagtgacc
     3781 gtgatcgggc gttcgaggaa gcgatttcca gcgatgcgcg tgtaggtgag attccccctc
     3841 tgggcggggc tgtcgaatat gagcctgagg cgttccccgc ctgaccatgt gagatcaatc
     3901 gtcaggcggt cggtttcgag agtggcgcgg acggtgacgt tctcgcctcc ggttgcacgc
     3961 atgatgcgcg tcttgccgtc ggcttcatac gggacgcgta tgccgtgcgg gtagttcatc
     4021 atgatggtcg tgccgtattg ctcgatggcg aagtgcggcg cggcgcggag cgttctgagg
     4081 agtgcgcgcc cctgtctctc acgctccccg tcatccagac gcgctgttgc gctcagcacc
     4141 ttccgcccga tctctgcgtc ctgactgttt ttctcccggt aggtgcccgt caggaaattc
     4201 tttccggctg gcggactaac ggcgttctgc gcctgcgacg cgatctgcat gttccggcct
     4261 tcaaaggctg gcgtggctgc cagcatgatg acgccgaatg ccaaaagccg gatcaacgtc
     4321 ctgtctcgaa atctcatctt cacgtccccg tcggggcttc gatacgactc tctctaacgg
     4381 aagagccgcc gggcgttccg ccttgatcgc tggcagctaa cttttgcgcg cgggcgagtg
     4441 caggcgagag ggatggaaaa agaggcgcgc ccgacctttg atgagccggg cgcgactcgt
     4501 gaaatgtttc ccttcgcaga gaaacgaaag tgtttatttg aaggtgacgc cgaaggagaa
     4561 gcgcaggttg tcctgacgct ggctttcgag ttgttgcgtc acaagcgcgc ctgccgctcc
     4621 gagcacctgc accgagcgat ctctgaggaa gacagggttg tagtcgaact ggatcagtcg
     4681 caacccgacg cgctcgttga ggcgcacatc gagtccgccg cctaaggcca tcgagaaatc
     4741 ggtggaactc gtctcgaagg tattctggac ggtgccggtg gcgagggtgc ggctctgctc
     4801 ggcctcgaag cgcgtattcg ccacgccgaa gagggcgcgg acgaagggcg tgacgcggct
     4861 ggtgttgggg aacctcatgg tcgggccgcc gaggaagctc accacacgcg tgtcgagttc
     4921 ggtgcgggct gagcctacgc cggtctggcc gttatccgag ttcaggttgc gcttgtgaaa
     4981 agcgaagtcg cccgtgaggc cgacgcgcgg cgtgaaaaag cccgtgactg caacgttgat
     5041 gccgtgcagt cctgaacgct ggccgaagag ttcgtcatcc agagagccgc cgccaccgcc
     5101 gcccggcatc gtgtcttcgc cggggatgcc ttcggcctgt aaattagaat agccgacgaa
     5161 gatctcaggc ccggttctgg ctgtcgtcgt gccctgagcg aaagtcagcg ggacgcacac
     5221 gagtagaatc gaggcgatga aaaagaattt gcgcatagaa aaatttcccc cttgaaacct
     5281 gcttgattga aattctgcgt gcttctcgcg ggtcgcgccc gcgccggaaa caaaagttcg
     5341 gagcgcacct gtctggcagt tgcgctccag gtcagttttg ctttgaggat agaagcatga
     5401 ggaatgtaga gagttttccc gcccgcgtct gtacgacctc gcacgaagga cgacatattt
     5461 cttaaggagc accccgcacg cgcccgacgc gcaagtcgag atgcggcggt tcgtctcgac
     5521 ccgctgagat tgacgcgccc gtttcgtcct tcgtttcgtc cttaacgccc ctgtcccgcc
     5581 gcttcgagcg cgagcggctt gcgctctgtg agagagaagc gcgcgccacg gggcaggatg
     5641 tgaacgcgta tgttatgaag ttcgattccc tcttccttcc gtatctcgtg caggttcgtg
     5701 tgggtaatgc ccccggcgtc gatgatcgtg accgccccgc tgccgatgac ctcgaattcg
     5761 ttcctgttga cgaccatcgc cgtgtcttcg tcaatgccga tgcccatgtc ctgcgggtag
     5821 tgcgccgtgg ccgtgatgag cctgccgtgt cgtccacgct gcgagaagtg cgtgtcgatg
     5881 agcgtcccca tgaggaaatc catgccggga ccaatctcga cagaaccgaa gcgcgggtta
     5941 gtctccgact cgccgccgag catcatggag ttagacatca tggccgcccc cgcgctcgtc
     6001 ccgccgagca ccaacccctt gccgaaacgc ttcttgatga tccgatccat ctcggtgccg
     6061 ccgagcagcg cggtgatgtg tagttgatcg ccgccggtga agaagaggcc ggtggcttcc
     6121 tcaatcgcat ccatcgcctt gctcgattcg gtgtcctcac gctgcgacac gtctacaacc
     6181 ttcacatcgg cggctccgag ccgtttgaaa atcttcttat agtccgcgcc cacctcttcc
     6241 ggatgatccg tcgcgacggt catcacgacg atgtgcgcct tatctccttt agcgagccgg
     6301 acgaactcct tgaggatgat gcaatctccc tctcgatctt ccgccccgcc gatgacgacc
     6361 agctttccgc gtgcctcgct cagttccatt attattcccc tctcatcgcc ccggtaaaac
     6421 tgccgcagca cataggctgc tccgtttccg atggcctcaa catctattca acctttagag
     6481 ataaaccacg tctgcccgct tttcaagccg ggcggctcca ttgtccagcc cctccccgcg
     6541 tctctcatct tatgaggaac ggggcgacaa cctattgcac gcccaccgac tcctgagaca
     6601 cagctaccgg ctgtcgccgc gcttctatcg cgccgaccgg gtgcgcgccg tattccctga
     6661 gcgcatcgag cacaggaccg agtttgtcgt agaagatgac gacgacctgc ccctcctgca
     6721 tctcctgtaa ctctgagcgc agtgcttcta cctcgtcgag cacgatgcga cattcggttc
     6781 ccggccactc atcgttcacg gcttcgcaca ggagcttggc gacctcgccc ttctcgcgcc
     6841 cgcgcaggtc tttgtcttcc ttgatgatga tgcgatgaaa gcctcgcgcg gcgacgcgcc
     6901 ccgcctgctc cacaaccgag tcgtcgcggt cgcccggcac gccgatgatg cctgtcacgc
     6961 gacggtcttc ccacctcgca gccatccggc agacggcgtc gaaggcatca gggttgtggc
     7021 cgtagtcaac catcacgtag ccgccgccga ccctgtacag gttggctcga ccggggttct
     7081 ccgcgtcgct gcggaagcgc gcgattgaag cccccacctc ttctctcgac aaaccgaacg
     7141 cacgcgctgc ggccacggct gccaacgcgt tggcgatctg gaagtcggcg gagccgttca
     7201 tcgtggccgg tatcgcggcg gcctcgatga acgcgtgctc gctgccgcct tcgagttcta
     7261 cgagccagcc gtctctgtag aagtaggccg tgccgcccgc gtcgcagtgt tggaggagaa
     7321 ccgggttgtc ctcccgtagc gagaagtaga cgtacttctt cggcacgcgg ctaacggcgg
     7381 ctcgctcgga gagacgcgcg agccgttcgt cgtcggcgtt gaggatgagc gtgccgccct
     7441 cccggacgcg ctctgccacg agcgacttga tccagaggat gtcctcgacc gattcgatgc
     7501 cgtcctgccc gatgtgatcc gcgccgacgt tcgtgatgac ggagatgtcc gaccagtcgt
     7561 agccgagtcc gcgacgcacg atgccgccgc gcgccgtctc cagcacggcg gcctcgattg
     7621 ctttgtccgt cagaatcgtc atcgccgaaa ccggccccgt cgtgtcgcca cggacgatgc
     7681 gctcgccgtt cagataaatt ccgtcggtcg tcgtcgtccc ggtcacgagt cccgcttcga
     7741 tcaggatatg gctgatcatg cgcgtgacgg tcgttttgcc gttcgtgccc gtgatggaga
     7801 tgatgggaat gcgcccgccg gaaccggcgg gaaagagcat ctcgacgatc tcgcgcccca
     7861 cgtcgcgcgg ttggccttcg ccgggatgct ggtgcatacg cagccccggt gcggcgttga
     7921 tctcgatgac gcccccgcct ttgccgacgg gcgcggcgat gtcatcgacg acgagatcga
     7981 cgccgcacac gtccatgccc atgaggcgcg cggcgcgttc gcagagactc ctgacgctcg
     8041 cgtgaacttc atccgttacg tctttggccg tcccgccggt cgagaggttc atcccggcgc
     8101 agagcatgac gcgctcgccc gccgccggga cagcatccat gctccatccc tctttgcgca
     8161 tgaaagtcgt caggatggga tcgtccttct tgatcttggt gagcggcttc tcgtgcccct
     8221 cgccgcgcag cggattctgg ttttcgatct cgatcaattc tgcgaccgtg tgcgtgccgt
     8281 cgcccatgac gtgacacggg atgcgctcgc tggccgcgac catccggtag ccgacgacga
     8341 gcacgcgata gtttcgcccc ccgaacatct cttcgaccag cacttcgcgc gaaaactcgc
     8401 gtgcgatgtg gaaggcgttg acaatctcgt ccggtgtcga gagatggagc gagacgccct
     8461 tgccctgtcg cccgtccaga ggcttgacga cgacgggcgc gccgattgtg ttgagagccg
     8521 cgacggcctc ttcctccgta tagacgatct caccttcggg gacggggatg gaagccttcg
     8581 ccagacgcgc cttcgtcagg tctttgtcgc ccgcgatctc cacgccaatc
//
LOCUS       DASWNS010000171         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_500736, whole genome shotgun sequence.
ACCESSION   DASWNS010000171 DASWNS010000000
VERSION     DASWNS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500736"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VGB76_05920"
     CDS             <1..891
                     /locus_tag="VGB76_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0098473.1"
                     /translation="LIWPLIALGYVVNLYQRGTASLKRMNAIFAIKPDIADEENAPEQ
                     PQIKGRIEFRNLTFSYSEHLPPVLRDIDLVIEAGQTVAFVGRTGTGKSTLMNLLPRMM
                     DAPRGTVLIDDVPVRDYPLKQLRSSIGYVPQETFLFSESLAENIAFGVERAERAEVEW
                     AAEIAGLSEDIRGFPEGFDTLVGERGITLSGGQKQRTAIARAVLRQPRILILDDALSS
                     VDTYTEEKILAQLRGVMRERTSLIVSHRVSTVRDADLICVLEEGRIIERGTHDELLAR
                     GGEYAALYERQLLEEELAAS"
     gene            974..1438
                     /locus_tag="VGB76_05925"
     CDS             974..1438
                     /locus_tag="VGB76_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098474.1"
                     /translation="MNRSAERMPAEARRRGLLLIWGAQLFSLALLFGLTYVVRPEAST
                     DGNHTLAWTLGAVGVSSFLLSFLVKRKLLARAAAERRPELGTTAYVLAFAMCEMAGLL
                     GFVAYVVTGLSYSLHSFIIAAAGILLHFPARGVFEEIEAGGNAGANLNQTTL"
     gene            1458..>2947
                     /locus_tag="VGB76_05930"
     CDS             1458..>2947
                     /locus_tag="VGB76_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0098475.1"
                     /translation="MATAVQQFHEEEAIGKTYDSQIARRLLRYLRPYLRLLIPSLILT
                     LALNGLRVLQPKFTQYAIDWHILPCQSGGGAACEPGGLVWLAAAMLGVLLLTFLFSYA
                     AAVLLNTIGQRVMYDLRAELYEKLQHQEVAYFDRHPVGRIMTRLTSDVDALNELFTSG
                     VTDVLGDLLTILIIIAIMLSMDWRLTLVTLITVPLLFAATSWFRKHARRGYDAVRTRL
                     ARINSFLQEHVSGAQTVQIFNREAKSERKFHELNDDYRRANIETIFYYAVFFPLVDLI
                     GAVGIALIVWYGGYRVMGTTPGAPQLLTLGALVAFIQYSQALFQPIRDISDKYNVLQA
                     AMVASHRLFKTLDLPIAITTPETPVKTGRARGRIEFDHVWFAYKGEDWVLKDVSFAVE
                     PGQSVALVGHTGSGKTTITNLLMRFYDVQRGRVLLDGVDVREWDLKALRENFAVVLQD
                     VFLFSGTVESNIRLGREEMPDERISWAAREVRADNFIEKLPEGFKS"
BASE COUNT          549 a          910 c          891 g          597 t
ORIGIN      
        1 ctcatctggc cgctcatcgc gctcggctac gtcgtcaatc tctaccaacg cgggacggcg
       61 agtttgaaac gcatgaatgc catcttcgcg atcaaacccg acatcgccga cgaggagaac
      121 gcgcccgagc agccgcagat caaaggccgc atcgagtttc gcaatctgac ttttagctac
      181 agcgaacact tgccaccggt tttgcgcgac atcgatctcg tgatcgaagc ggggcagacc
      241 gtcgcgttcg tcgggcgcac gggcacgggt aagtctacgc tgatgaatct gctgccgcgc
      301 atgatggacg cgccgcgcgg cacggtcttg attgacgacg ttcccgtgcg cgactacccg
      361 ctcaagcagt tgcgttcgag catcggttac gtcccgcagg agacttttct cttcagcgaa
      421 tcgctcgccg aaaacatcgc ctttggagtt gaacgtgcgg agcgcgcgga ggttgaatgg
      481 gcggcggaga tcgcgggctt gagcgaagac atacgcggct ttcccgaagg tttcgacacg
      541 ctcgtcggcg agcgcggcat cacgctctcg ggcgggcaga agcaacgcac ggcgattgcc
      601 cgcgccgtgc ttcgtcaacc gcgcatcctg attttagacg acgcgctctc cagcgttgac
      661 acttacaccg aagagaaaat cctcgcgcaa ctgcgcggcg tgatgcgcga gcgcacgagt
      721 ctcatcgtct cgcatcgcgt ctccaccgtg cgcgacgccg atctcatctg cgtgctggaa
      781 gaggggcgca tcatcgaacg cggcacgcac gacgaactgc tcgcgcgcgg cggcgaatat
      841 gccgcgctct acgagcggca actgttggaa gaagaactgg ccgcgagttg aagttcggct
      901 cagagggtaa gggttgaagt agtttttagt ttaggctgtt gattgatggc ggccacaatc
      961 ggaggaaggg ttgatgaatc ggagtgcgga aagaatgccc gccgaagcgc gtcggcgcgg
     1021 gctgttgctc atctggggcg cgcagttgtt ctcgctcgcg ttactgttcg gcctcaccta
     1081 cgtggttcga ccggaggcga gcacggacgg caatcacacg ctcgcatgga cgctcggcgc
     1141 agtcggcgtc agtagtttcc tgctctcttt tctggtaaag cgaaagttgc tggcgcgggc
     1201 tgccgccgag cgtcgccctg agttggggac gacagcttac gtgctcgcct tcgccatgtg
     1261 cgagatggcg gggctgctcg gcttcgtggc ttacgtcgtc acggggctgt cctactcgct
     1321 gcactccttc atcatcgcgg cagcagggat actcctacac tttcccgcgc gcggcgtgtt
     1381 tgaagagatc gaagcgggcg gcaacgcggg cgcgaatctt aatcagacga ctttataacc
     1441 ggcggaatta tcgaaacatg gcgacagcag ttcaacaatt tcacgaagaa gaggccatcg
     1501 gcaaaactta cgattcgcag atcgcgcgtc gcctcctgcg ctacctgcgc ccttacctgc
     1561 ggctcctcat cccctcgctc atcttaactc tggctttaaa cggcctgcgc gtgctgcaac
     1621 cgaagttcac gcagtatgcg attgactggc acattctgcc gtgccagtcg ggcggcggag
     1681 cagcgtgtga accgggcggc ctcgtctggc tggcagcggc gatgctcggc gtgctgctct
     1741 taactttcct cttctcttac gcggctgccg tcctgctcaa caccatcggc cagcgcgtga
     1801 tgtacgacct gcgcgcagaa ctctacgaga aactgcaaca tcaggaggtg gcctacttcg
     1861 accgccatcc cgtcgggcgc atcatgacgc gcctcacttc cgatgttgac gctttgaacg
     1921 aactgttcac ctccggcgtg acggacgtgc tcggcgatct gctgaccatc ctgatcatca
     1981 tcgccatcat gctctcgatg gactggcggc tcacgctcgt cacgctcatc accgtgccgc
     2041 tcctgttcgc cgcgacctcc tggtttcgca agcacgcgcg gcgcggttac gacgccgtgc
     2101 gcacgcgcct tgcgcgcatc aactcgtttt tgcaggaaca cgtttcgggc gcgcagaccg
     2161 tccagatttt caaccgcgag gccaaatccg agcgcaagtt tcacgaactc aacgacgact
     2221 acaggcgcgc caacatcgag acgatctttt actacgcggt cttcttcccg ctcgtcgatc
     2281 tgatcggcgc ggtcggcatc gcgctcatcg tctggtacgg cggctatcgc gtgatgggca
     2341 cgacgcccgg cgcgccgcaa cttctgacgc tcggcgcgct cgtcgccttc atccaatatt
     2401 cacaggcgtt attccagccg atccgggaca tctctgataa gtacaacgtc ttgcaggcgg
     2461 cgatggttgc ctcgcatcgc ctcttcaaga cgctcgacct gcccatcgcc attacgacgc
     2521 cggagacgcc cgtcaagacg gggcgcgcac gcggtcgcat cgagttcgac cacgtctggt
     2581 tcgcttacaa aggtgaagac tgggtcttga aagatgtgtc gttcgcagtc gagccgggtc
     2641 aatcggtcgc gctcgtcggg cacacagggt cgggcaagac gacgatcacg aatctcctca
     2701 tgcgcttcta cgacgtgcag cgcgggcgcg tgctcttaga cggcgtagac gtgcgcgagt
     2761 gggacttgaa agcactgcgc gaaaacttcg cggtcgtgtt gcaggacgtg tttctcttta
     2821 gcggcacggt cgagagcaac atccggctcg gtcgggagga gatgccggac gagcgcatca
     2881 gttgggctgc gcgcgaggtg cgcgccgaca acttcatcga aaaactaccc gaaggcttca
     2941 agtcgga
//
LOCUS       DASWNS010000172         6188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_111722, whole genome shotgun sequence.
ACCESSION   DASWNS010000172 DASWNS010000000
VERSION     DASWNS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111722"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="VGB76_05935"
     CDS             complement(<1..1565)
                     /locus_tag="VGB76_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0098476.1"
                     /translation="MTTNTTGSNRSSSRSNGDGAPFLEMRDITKVFPGVRALDGVSFD
                     LRRGELHALVGENGAGKSTLMKILGGVYPHPEYGGEIFIEGEARRFKGVRDAEGAGVA
                     VIFQELSLVQEMTIGENIFLGREPRRFGIIRWNELYSRAGRLLADLHLPLDPHTPVRN
                     LGIGQQQLVEIAKALAQEARLLVLDEPTAALTDAEVETLFSILNDLRARGVGMIYISH
                     KLDEVFRLSDRITVLRDGRTVGTEQTSALTEASVIARMVGREVGDIFPVPEHAPGQVL
                     FEARGFTVEDPGVAGKLLVDGVSFEVRRGEVLGIAGLMGAGRSELLMAIFGAHVGRAR
                     GEVFIEGKRVRIENPADAIRHGLGFVTEDRKRFGLVLDQTILNNMTLAGLTRISGRFL
                     TDVDAEAAAGNRSMRELRVKANSVFTIAGTLSGGNQQKVVLAKWLLTNPRVLFLDEPT
                     RGIDVGAKQEIYAQINALAKSGLAIVLVSSELPEVLGMSDRVLVLHEGRVTGEFARSE
                     ATPEKVMSCATGHA"
     gene            complement(1730..2851)
                     /locus_tag="VGB76_05940"
     CDS             complement(1730..2851)
                     /locus_tag="VGB76_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0098477.1"
                     /translation="MRRFTSLLLLLLAATLLAASACTSGTGDNAAGGNTNGGAQTARK
                     KKGANEQIFIGFLMDTLKEERWQRDKELVEKRAQEAGAKLSVQVANGDDNLQIKQAEN
                     LLSQKVDVLIVAPHNGEIAATIVEMAHREGVPVISYDRLIRNADVDLYLSHQVVKMGE
                     MQARYLLERAPKGNYIVVQGSATDNNARLLHEGQMNVLQPAVDRGDIKIVVDQPAREW
                     LASEALNIVENALTQVNNDVVAVVASNDGTARGAIQALDGQKLAGKVLVSGQDADLAS
                     LQLILQGKQTMTVYKPIQPLAYGAVDAAMKLARGESVQTNDTVDNGKIKVPSMLFEPQ
                     AVDKNNLVATVVKDGYQTLERICQNAPEQCPKVDSPGAQ"
     gene            3294..>6188
                     /locus_tag="VGB76_05945"
     CDS             3294..>6188
                     /locus_tag="VGB76_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0098478.1"
                     /translation="MSSVRCNSLRAVLCGLFFLLFAATADAQFRAAIQGSVTDPQGGT
                     VVGATVTLTNNETGRTQQTTTGDEGFYRFAGLPPARYTLTVEQTGFNKKTLDNVLVNA
                     EATEGINITLETTGVMADVTVTDVLASPLETENAHINKAITTQEIRQLPQFGRDPYEL
                     LRLTPGVFGQGARSGSGGSVNLPNQSGPGGSNRAIFQTENQVQISANGQRVSANNFQI
                     DGTSVNSLTHGGAAVITPNQESVKEVRVIANNYSAEYGRNSGAQVLTVSQNGTNDFHG
                     SLFLKNNSPGLNAFNKYGGFNNAPTVRVNNRLNQFGGSIGGPLPLPRFGEGAPPAFEL
                     ARNRAFFFFSYEGLRSSNTDSVNAFVETPEFRNLILRTRPNSIAARILGAPGITPRVT
                     SVIPVTCAAAGFPFGSFTNCQQVAGGLDIGSPAGATGQYLPFSSLSGGGLDGIPDLQF
                     AQLAVPTITRGNQFNPRLDFNLGERDTIAVSGYLSRFRGVGSDSPGRSRAMGDIATAP
                     NNSFGMVTWTRTLSPTMLNELRFNATRYAYNEIESSSETPFNLPRIEIENMPGDRLRF
                     GPPRAETTPGIFAENTFEFRDTLRWVRGTHGLSFGGELRKEQDNNNLSGGARPLYTFA
                     GLFNFTNDAPLFYSINADPRTGGPADAQRYFRTDTYAFFAQDDWKARPNLTLNIGLRW
                     EYFSPLREKRGRLSNFILGPAGQELTGGRIAVVDELYEPDRNNFAPRLGFAWSPSQIL
                     GTDFQNKMVIRGGFGIAYNRLPVNVFENTRGNPPFFARYNICCGTSSQDFSTPFAGGQ
                     ILYATGASNSPFSYPFNPALALTFNPANGIPIVRPGSEVEIYGAPQDVSTPYVYTYSL
                     EAQYALPGNLSAEVGYQGSSSRKLVRLVDQRFLYANDPNSFFASRIIFPLADTNANYN
                     ALLARLSRRFSRGFQFDAHYRFSKSIDVVSSEQIGAPNPTYPLDPRQERGP"
BASE COUNT         1128 a         2062 c         1789 g         1209 t
ORIGIN      
        1 gcatgccccg tcgcgcacga catcactttt tccggcgtcg cttcgctgcg cgcgaactcg
       61 cccgtcacgc gcccttcgtg caggacgagc acgcggtcgg acatgccgag cacctccggc
      121 aactccgagg agacgagcac gatggcgagg ccggacttgg cgagcgcgtt gatctgcgcg
      181 tagatctcct gcttcgcgcc cacatctatg ccgcgcgtcg gttcgtcgag aaagagcacg
      241 cgcgggttcg tcaagagcca cttggcgagc acgaccttct gctggttgcc gcccgagagc
      301 gtgcccgcga tggtgaagac cgagttggct ttcacgcgca actctcgcat cgagcggttg
      361 cccgccgccg cttccgcgtc aacgtctgtc aggaagcgac cggagatgcg cgtaagcccg
      421 gcgagcgtca tgttattcaa gatcgtctgg tcgaggacga gtccgaagcg tttgcgatct
      481 tcggtgacga agccgagtcc gtggcggatc gcgtccgccg gattctcgat ccgcacgcgc
      541 ttgccctcga tgaacacttc gccgcgcgcg cgtccgacgt gtgcgccgaa gatcgccatc
      601 aggagttcgc tgcgccccgc gcccatcaaa cccgcgatgc ccaagacctc gccgcgccgc
      661 acctcgaaac tcacgccgtc aaccagcaac tttcccgcca cgccggggtc ttcgacggtg
      721 aagccgcgcg cttcaaagag cacttggccc ggcgcgtgtt ccggcacggg gaaaatatcg
      781 cccacttcgc gcccgaccat ccgcgcgatt accgacgctt ccgtgagcgc gctcgtctgt
      841 tcagtgccga ccgtgcgccc gtcgcgtaag accgtgatgc ggtcgctcag gcgaaacacc
      901 tcgtcgagtt tgtgcgagat gtagatcatg ccgacgccgc gtgcgcgcag atcattcaag
      961 atgctgaaga gcgtctcgac ctccgcgtct gtgagcgcgg cggtcggttc gtcgaggacg
     1021 aggaggcgcg cttcctgcgc gagggctttg gcgatctcga caagttgctg ctggccgatg
     1081 cctaagtttc tgacgggcgt gtgcgggtcg agcgggagat ggaggtctgc gagcaggcgg
     1141 cctgcacgcg agtagagttc gttccagcgg atgatgccga agcggcgcgg ctcgcgcccc
     1201 agaaagatgt tctcgccgat ggtcatctcc tgaaccagag atagttcctg aaagatgacg
     1261 gcgacgcccg cgccttcggc gtcgcgcacg cccttgaacc gccgcgcttc gccttcgatg
     1321 aaaatctcgc cgccgtactc cggatgcggg tagacgccgc cgagaatttt catcagggtt
     1381 gacttgcccg cgccgttctc gccgacgagc gcgtgcaact cgccgcgccg cagatcgaac
     1441 gagacgccgt cgagcgcgcg cacgccggga aagaccttcg tgatgtcgcg catctcaagg
     1501 aaaggcgcgc cgtcgccgtt gctgcgactg ctgctgcggt tgctgccggt cgtgttcgtc
     1561 gtcatctcgt cacaggcgtt cgggcggggg cgttcgtgtt caatcagccc tgcgagttct
     1621 ctctgcgcct ttgcgtgagt ttattcttta gtaactatta acctcacgca aaggcgcaaa
     1681 ggaaagcaga gagtagtttt cagacgcgcg cttaattcgt cgcacgcgtt tattgcgctc
     1741 cgggggagtc caccttcggg cattgttcgg gggcgttctg gcagattctt tcgagcgtct
     1801 ggtagccgtc tttcacgacc gtcgccacca gattattttt gtccacggcc tgcggctcaa
     1861 acaacatcga aggcactttt atcttgccgt tatcaaccgt gtcgttcgtc tggacgcttt
     1921 cgccgcgcgc aagtttcatc gccgcgtcaa ccgcgccgta ggccagcggt tgaatcggtt
     1981 tgtagaccgt catcgtctgc ttgccctgca agatcaactg gagcgaggcg aggtctgcat
     2041 cctgcccgga gactaaaact ttgcccgcga gtttctgccc gtcgagagcc tgaatcgcgc
     2101 cgcgcgccgt gccgtcgttg gaagcgacga cggcgacgac atcgttgttg acctgtgtga
     2161 gcgcgttctc cacgatgttc agagcctcgc tcgccagcca ttcgcgcgcc ggttgatcga
     2221 cgacgatctt gatgtcgccg cgatccactg cgggctgcaa gacgttcatc tgcccctcgt
     2281 gcaagaggcg cgcgttgttg tccgtcgcag aaccctgcac cacgatgtaa ttgcccttcg
     2341 gcgcgcgctc caacaaataa cgcgcctgca tctcgcccat cttcacgacc tggtgcgaga
     2401 gatagaggtc aacgtcggcg ttcctgatca agcgatcata actgatgacg ggcacccctt
     2461 cgcggtgcgc catctcgaca atggtcgccg cgatctcgcc gttgtgcggc gcgacgatga
     2521 gcacgtcaac tttctgcgag agcaggttct cggcctgctt gatctgcaaa ttgtcgtcgc
     2581 cgttcgccac ctgcacggag agtttcgcgc ccgcctcctg cgcgcgcttc tccaccaact
     2641 ctttatcgcg ctgccagcgt tcctctttca gcgtgtccat caggaagccg atgaagatct
     2701 gttcattcgc ccccttcttc ttgcgcgcgg tttgcgcgcc gccgttcgtg tttccaccgg
     2761 cagcgttatc gcctgtgccg ctcgtgcaag cggaagcggc cagcagcgtc gctgccagca
     2821 gcaacaacag tagagatgtg aatcttcgca tgagttaagc tccttgaatt tttacgtgtg
     2881 cgattctcaa gttcaaagcg tttagggcgc ggtctgcggc gcgggggttc cccgccgtct
     2941 gtaaagcgag acgacagatg ccacactcga tctcgcgcgg gctgttggat cagttgaaaa
     3001 tgaaaacagg ctgatcggtt gggcgtagct taacaaaggc gcggcgcata tttccattga
     3061 atttttcccg tgcggatgtt aatttgcgcc tattcgaacc gttcccttta actttgcaag
     3121 caatcttggc cgccaaaggt gcggcgttaa tccgcgaccg agttatcaaa ccggcgtcgt
     3181 ggagttgtag gtcaacaact ttctgatcct gcgtggttcg aaggctcgac aggcgcagca
     3241 cgaaagctgc gcgcctcgct tggacaggaa gcctctcagg aagggtttaa atcatgagtt
     3301 cagtgcggtg caattctctt cgcgcagtgt tgtgcggact cttcttcctc ctcttcgccg
     3361 ccactgccga cgcgcagttt cgggcggcca ttcaaggctc tgtgacagac ccgcaaggcg
     3421 gcacggtcgt cggcgcaacc gtcacgctca ccaataacga aaccggcagg acgcaacaga
     3481 cgaccaccgg cgacgaaggc ttttatcgct tcgccgggct gccgcccgcg cgctacacgc
     3541 tcaccgtcga gcagacgggc ttcaacaaga agacgctcga caacgtgttg gtcaacgccg
     3601 aggcgaccga aggcatcaac atcacgctgg aaaccaccgg cgtgatggcc gacgtgacgg
     3661 tgacggacgt gctcgcttcg cccctcgaaa ccgaaaacgc tcatatcaac aaagcgatca
     3721 ccacgcagga gatacgccag ttgccgcagt ttgggcgcga cccttacgag ttgctgcgcc
     3781 tgacgcccgg cgtcttcggg cagggtgcgc gcagcggttc gggcggctcg gttaatctgc
     3841 ccaaccagtc aggacccggc ggctcgaatc gcgccatctt ccagaccgaa aatcaggtgc
     3901 aaatctctgc caacggccag cgcgtctcgg ccaacaattt tcagatcgac ggcacgagcg
     3961 tcaacagcct gacgcacggc ggcgcagccg ttatcacgcc gaatcaggaa tcggtcaaag
     4021 aggtacgcgt catcgccaac aactactctg cggagtacgg gcgcaactcc ggcgcgcagg
     4081 tgctcaccgt gtcgcagaac ggaacgaacg attttcacgg cagcctcttt ctcaagaaca
     4141 acagtccggg cttgaacgcc ttcaacaagt acggcggctt caataacgct cccaccgtcc
     4201 gcgtcaacaa ccggctcaat caattcggcg gctccatcgg cggccccctc ccgctcccgc
     4261 gcttcggcga aggcgcgccc ccggccttcg agttggcacg caatcgcgcc ttcttcttct
     4321 tctcttacga aggcttgcgc agcagcaaca cagactccgt caacgccttc gtcgagacgc
     4381 cggagttcag aaatctgatt ctccgcacgc gaccgaacag catcgccgcg cgcatcctcg
     4441 gcgcacccgg catcacgccg cgcgtcacga gcgtcatccc cgtcacctgc gccgccgccg
     4501 gtttcccttt cggttcgttt accaactgcc agcaggtggc gggcgggttg gacatcggct
     4561 cgcctgccgg tgcgaccggg caatatcttc ccttcagcag tctgtccggc ggtggcctcg
     4621 acggcatccc cgatctacag tttgcccaac tcgccgtgcc gaccatcacg cgcggcaacc
     4681 agttcaaccc gcgtctggac ttcaacctcg gcgaacgcga cacgattgct gtgagcggtt
     4741 acctctcgcg ctttcgcggc gtcgggagcg acagcccggg caggagtcgc gcgatgggcg
     4801 acattgcgac cgccccgaac aacagtttcg gcatggtgac ctggacgcgc acgctctcgc
     4861 cgacgatgct caacgagttg cgcttcaacg ccacgcgcta tgcctacaac gagatcgagt
     4921 cgtcgagcga gacgcccttc aacctgcccc gcatcgagat cgagaacatg ccgggggatc
     4981 gtctgcgctt cggcccgcca cgcgccgaga cgacgcccgg catcttcgcc gaaaacactt
     5041 tcgagtttcg cgacacgctg cgctgggtgc gcggcacgca cggcctgagc tttggcggcg
     5101 aactgcgcaa ggagcaggac aacaacaacc tgagcggcgg cgcgcgcccg ctctacacct
     5161 tcgccggact cttcaacttc accaacgatg cgccgctctt ctactccatc aacgcagacc
     5221 cgcgcaccgg cggacccgcc gacgcgcaac gctacttccg cacagacacc tacgccttct
     5281 tcgcgcagga cgactggaag gcgcgcccga atctcacgct caacataggc ttgcgctggg
     5341 aatatttctc gccgctcagg gagaagcgcg ggcggctcag caacttcatc ttaggcccgg
     5401 cgggtcagga attgacgggc ggcagaatcg ccgtcgtaga cgaactctac gagccggatc
     5461 gcaacaactt cgcgccgcgt ctcggcttcg cgtggagtcc aagtcagatt ctcggaaccg
     5521 atttccagaa caagatggtc attcgcggcg gcttcggcat cgcctacaat cgcctgcccg
     5581 tcaacgtctt cgagaacacg cgcggcaacc cgcccttctt cgcgcgctac aacatctgct
     5641 gcggcacttc gtcgcaggat ttcagcacgc cgttcgcggg cgggcagatt ctctacgcca
     5701 cgggcgcgag caactcgcct ttcagctatc cgttcaaccc tgcgctggcc ttgacgttta
     5761 atccggccaa cggcattccc atcgtccgtc ccggctcgga ggtggaaatc tacggcgcac
     5821 cgcaggacgt ttcgacgccc tacgtctaca cctattcgct cgaagcgcaa tatgctctgc
     5881 cgggcaatct cagcgcggag gtcggctacc agggcagttc gagccgcaag ctcgtgcggc
     5941 ttgtcgatca gcgtttcctc tacgcgaacg accccaacag cttcttcgcc tcgcgcatca
     6001 tcttcccgct cgccgacacg aacgccaact acaacgcact gctcgcgcgc ctctcgcgcc
     6061 gcttctcgcg cggcttccag ttcgacgcgc actaccgttt cagcaagagc atcgacgtag
     6121 tttcgagcga gcagatcggc gcgcccaacc cgacctaccc gctcgacccg cgacaggaac
     6181 gtggtcct
//
LOCUS       DASWNS010000173         7145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_85238, whole genome shotgun sequence.
ACCESSION   DASWNS010000173 DASWNS010000000
VERSION     DASWNS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85238"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VGB76_05950"
     CDS             <1..494
                     /locus_tag="VGB76_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0098479.1"
                     /translation="DETKLDPRSLKLEITESVVMENIDTATEMLKQLRALGVQLSIDD
                     FGTGYSSLSYLHRFPIDTLKVDRSFVMRMVDNNENIEIVRTILMLAQNLGMDVVAEGV
                     ETKEQLALLRKLGCENGQGYFFSRPVSVGGAEKIIADTYSHAESSPDKQPNVRAIRPA
                     MVA"
     gene            542..892
                     /locus_tag="VGB76_05955"
     CDS             542..892
                     /locus_tag="VGB76_05955"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0098480.1"
                     /translation="MAERRQAERVYVRLNARWEGVLAQCEGHLVDLSATGCFVLTSDR
                     VKTKELIRVEIQLPTEGWMYLWGEVVYRIEDMGFALRFTGVSEMEQAMLNLLLEFVRD
                     EASSSVAAPVLATK"
     gene            1084..2127
                     /locus_tag="VGB76_05960"
     CDS             1084..2127
                     /locus_tag="VGB76_05960"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY0098481.1"
                     /translation="MADDLTMRYIENPPNPWNTTHVEWIGEPPEAAQEIFEEEATRSI
                     ITKNDSPDVGFDYSVNVYRGCTHACTYCFSRPTHEYLGFGAGTDFERKLVVKIHAAEL
                     LRRELMRPSWKGDELVFSFTSDPYVPLEAHYKLTRRCLEVCLEFRNPVSIVTKSALVR
                     RDLDLLVELARDASVEVYFTIPFADAATARALEPFAPAPDARFKAMAELAAAGIPVGI
                     GIAPVIPTLNDAHIPQLLKRAREAGARHAFMTLLRLPGSVAPYFEQRLREGLPTKADR
                     ILKRLREMRGGRLNSSEFGERMRGQGEYWRMIEQSFRLHCERNNFNRHGDVLAVTPRR
                     TFRRPSPQGLLFE"
     gene            2247..2672
                     /locus_tag="VGB76_05965"
     CDS             2247..2672
                     /locus_tag="VGB76_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0098482.1"
                     /translation="MMTIDDVIREREPYFVRESATVLEAAKFMTNLNIGAVCVLDDEG
                     RLRGIFSERAVLRRVVIAGKDPATTKVGEIMSVIKAVIECKNTPHEALELMEKHGTRY
                     LPVVRNDRWVGMLAMRDIMRVELGEQGDELRLLHEYIQQ"
     gene            complement(2808..3836)
                     /locus_tag="VGB76_05970"
     CDS             complement(2808..3836)
                     /locus_tag="VGB76_05970"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY0098483.1"
                     /translation="MLEEIAEQPEALRRTIDAERTKVERLGAFLRERGVSLIVLVARG
                     SSDNAALFGRYLLEVATGIPVSLSAPSVHTLYHARLNLRGALVVGVSQSGEGEDINRV
                     LENARAGGAYTVGITNEANSAMVSLVDETLLMHGGRERSVAATKTFTGQILHFHMLAA
                     ALAAGRAEHTNFDAIPEMAARALELRAAVAEIVERYVFMENCVVVGRGLLYANAYEMA
                     LKLMETCYVVAERFSSADFLHGPLAMVERHFPVILFAPPGVTVESTRTLLARLRELRA
                     DTLVITGDDEAARTATRALRLPPDINEFLAPIPYIIPAQLFAALLAEAKGLDPDAPRS
                     LSKVTRTL"
     gene            complement(3869..4489)
                     /locus_tag="VGB76_05975"
     CDS             complement(3869..4489)
                     /locus_tag="VGB76_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0098484.1"
                     /translation="MWNDPTQAVRAQLESGEQLIWSGQPRQGLLLRPSDAFMIPFSLL
                     WGGFAIFWEVSAILSGAPIFFWLFGVPFVLIGLHLIFGRFFTDARTRTHTFYGLTNDR
                     IIITSGKHGKRVQSLSLSTLSDISLDERGDGSGTITFAPASPFGRWSNFGALPGATRG
                     QTPPSLEMIPQARKVYEMIRGAQRRLHSGVRRESLNDSTGNDLASL"
     gene            4583..6748
                     /locus_tag="VGB76_05980"
     CDS             4583..6748
                     /locus_tag="VGB76_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HEY0098485.1"
                     /translation="MQKSHGHILTRRLVSRPAFLPCQHAFLAGWLCLALLLLASSVAR
                     AQTDDATRDVRVIPQPRQLTLKPERFRLTRDVRLVLAAPRSAEDRFAAEDFAADVRET
                     AGGLALSIGRSRRRASILIGALDLASVQSALRRANVSAPPDLNEEGYVLSVSADEVVV
                     AGKTAAGTFYGLQTLKQLVRGDASEAFLPGVRIVDWPQMRWRALSDDISRGPVPTVEY
                     IKRQLRTLAAFKMNMHSFYMEHTFGSRSHPLVAPVGGSLTPEEIRELFAYARRYHIEL
                     VPEQQTFGHLHKALKFEKYNELAEVPYGDVLSPQQEGSFKLVADWYRELDELFPGKFF
                     HIGADETFELGEGQSREQVKARGVGAVYFEHLRRVREILQPYNRRLMFWGDIALNHPE
                     LIGSIPKDLIVMNWAYGARQDFAARIKPFKDAGLEQFVCPGVSNWNQVFPHLDNAAKN
                     ITGFVRDGQRAGVLGMMNTNWDDDGETLFEMAWYGVVLGAAASWQEGEVDLSQFERNF
                     DWAFFRHDGEEFVRALRTLGSVNTTLGINTSNPLFWQEPFTNAFQERSRGLREKTREM
                     RRAVEGVEEMLAREGAKARRNRQSIASMRFAAQRLDHMGRRMQVVEGFSRDYWQAYLN
                     LGDRRQVNRLRRYHGAIYNNLREMAEELATLKASYREQWLAENRPYWLDSVLARYDQA
                     IATWLGRSKALEEAMREYNESSTLPNPEEFGLGSRPDQP"
     gene            6897..>7145
                     /locus_tag="VGB76_05985"
     CDS             6897..>7145
                     /locus_tag="VGB76_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEY0098486.1"
                     /translation="MMDVPDADQRGGCGAERLLSLDVFRGLTIAGMILVNNPGPSGRV
                     YWPLEHAAWHGWTPTDLIFPFFLFIVGVSITLAFARRVE"
BASE COUNT         1430 a         2147 c         2212 g         1356 t
ORIGIN      
        1 tggacgagac gaaactcgac ccgcgcagcc ttaaacttga gatcacggaa tcggtcgtga
       61 tggaaaacat cgacacggcg accgagatgc tcaagcaact gcgcgcgctc ggcgttcaac
      121 tcagcattga cgatttcggc accggttatt cgtcgctcag ttatctacac cgtttcccga
      181 ttgacactct gaaagttgat cgctcgttcg tcatgcggat ggtggacaac aatgagaaca
      241 tagagatcgt ccgcaccatc ctgatgctgg cgcaaaatct gggcatggac gtggtggccg
      301 aaggcgtgga gacgaaagag caactcgcgc tcctgcgcaa actcgggtgc gaaaacggac
      361 agggatattt cttctcgcgc ccggtcagcg tgggcggcgc ggagaagatc attgccgaca
      421 cctattcgca tgcggagagt tcgcccgaca agcagccgaa cgtgcgcgcg attcgtccgg
      481 cgatggtggc ttgagataga taggatgcag cgataaataa gatataaagg gaaagggaat
      541 tatggctgaa cgcagacagg cagaacgtgt ttatgtacga ctcaatgcgc gttgggaagg
      601 cgtcttggcg cagtgcgaag gccacctcgt cgatctcagc gcgacgggat gttttgtact
      661 gacttcagat cgtgtcaaga cgaaggaact gatccgcgtg gagattcaat tgccgaccga
      721 aggctggatg tatctctggg gcgaggtggt ctaccgcatc gaggacatgg gattcgcgct
      781 acgctttacg ggcgtgagcg agatggaaca ggccatgctc aatctcctgc tcgaattcgt
      841 tcgagacgag gccagcagca gcgtggccgc tcccgtatta gcgacgaaat aagagccgct
      901 gtagtcgccg ctcattcata agatcaaata gcccggtcgg cacgtgacag agttttcgct
      961 tcgcgactct tcgactgcca gccgggcttt cgcttgtcgt ttacccgttc ggcgcgtggg
     1021 cgcgacctct ggcgttgacg ctcggccaaa atcgttatat ctctaactcg cgaaccgagg
     1081 agcatggccg acgatttaac gatgcgctac attgagaatc ccccgaaccc ctggaacact
     1141 acgcacgtcg aatggatcgg cgagccgccg gaagccgcgc aggagatctt cgaggaagag
     1201 gccacgcgct ccatcatcac gaagaacgat agccccgacg tgggcttcga ctacagcgtc
     1261 aacgtctatc gcggctgcac gcacgcctgc acctattgct tctcgcgccc cacgcacgaa
     1321 tatctgggct tcggcgcggg cacggatttc gagcgcaaac tcgtcgtcaa gattcacgcg
     1381 gcggagttgc tgcgcaggga actcatgcgc ccttcttgga agggcgacga actcgtcttc
     1441 tctttcacct ccgaccctta cgtgccgctc gaagcccatt acaagttgac gcgcaggtgt
     1501 ctggaagtct gccttgagtt tcgcaatccg gtgagcatcg tcaccaagtc ggctctggta
     1561 cggcgcgatc tagacctgct cgtcgaactc gcgcgcgacg cctccgtcga agtttacttc
     1621 acgatccctt ttgccgacgc cgcgacggca cgcgcgctcg aaccgtttgc gcccgcgccc
     1681 gatgcgcgtt tcaaagcgat ggcggaactt gccgccgcag gcatccccgt cggcatcggc
     1741 atcgcgcccg tcatcccgac gctcaacgat gcgcacatcc cgcaactctt gaagcgtgcg
     1801 cgcgaggcgg gcgcgcgtca tgcttttatg acgctcctgc gtctccccgg cagcgtcgcg
     1861 ccctacttcg aacaacgctt gcgcgaaggg ttgccgacaa aggccgaccg cattttgaag
     1921 cggctgcgcg agatgcgcgg cgggcgtctc aattcgagcg agttcggcga gcgcatgcgc
     1981 gggcagggcg aatactggcg catgatcgaa cagagttttc gcctgcactg cgagcggaac
     2041 aatttcaacc ggcacggcga tgtcttagcg gttacaccgc gccgcacctt tcgtcgcccg
     2101 tcgccgcaag gcttgctctt cgagtagaga aaatttgtac aagcgcggcg gtcaagacta
     2161 aacgcttgat cttctcgcgg cgaagattta caacacaggc agttcctaca aaaccctgca
     2221 cgcatcacga cggaggtaca cctgaaatga tgaccattga cgatgtgata cgcgagcgcg
     2281 agccatattt tgtacgtgaa tcggcgaccg tgctcgaagc ggcgaagttt atgacgaacc
     2341 tcaacatcgg ggcggtctgc gtgctcgacg acgaagggcg tctgcgcggc atcttctccg
     2401 agcgcgcggt gctgcgccgc gtcgtcatcg cggggaaaga ccctgcgacg acaaaggttg
     2461 gcgagataat gagcgtgatc aaagccgtca tcgaatgcaa gaacacgccg cacgaagctc
     2521 tggagttgat ggagaaacac ggcacgcgtt acctgcccgt cgtgcgcaat gatcgttggg
     2581 tcggcatgct cgccatgcgc gacatcatgc gcgtcgaact cggcgagcag ggcgatgagc
     2641 tacgcttgct gcacgagtac atccagcaat agaggagtga caagtgacga gtgacaagtg
     2701 acgagaaaag ccgtgaatcg tgaatcgtga atagaggaac tgctttcttc tattcacgat
     2761 tcacgattta cgattcacgg ctttttaact tgtcactcgt cactgcttta tagcgtgcgc
     2821 gttactttcg agagcgagcg gggcgcgtcc gggtcgagtc ccttcgcttc tgcgaggagt
     2881 gcggcgaaga gttgggcggg gatgatgtag gggatgggcg cgagaaattc gttgatgtcg
     2941 ggcggcaggc ggagcgcgcg cgtggcggtt cgtgcggctt cgtcgtcgcc ggtgatgacg
     3001 agcgtgtcgg cgcgcagttc acggaggcgc gcgaggagcg tgcgcgtgct ctcgacggtg
     3061 acgccaggcg gggcgaagag gatgacgggg aagtggcgtt cgaccatcgc cagcggcccg
     3121 tgcagaaagt ctgcggaaga gaagcgttcg gcgaccacgt agcaagtctc catcaacttg
     3181 agcgccatct cgtaggcgtt cgcgtagagc aacccgcgcc cgacgacgac gcagttttcc
     3241 atgaagacgt aacgctccac gatctctgcg accgccgcgc gaagttcgag cgcgcgtgcg
     3301 gccatctcgg gaatcgcgtc gaagttcgtg tgttcggcgc ggcctgcggc gagcgcggcg
     3361 gcgagcatgt ggaagtggag tatctgcccg gtgaaagttt tcgtggcggc gacggagcgt
     3421 tcgcgcccgc cgtgcatcaa gagcgtctcg tcaacgagcg acaccatcgc ggagttcgct
     3481 tcgttcgtga tgccgacggt gtatgcgccg cccgcgcgcg cgttctcaag gacgcggttg
     3541 atgtcttcgc cctcgcccga ttgcgagacg ccgacgacga gcgcgccgcg cagattcagg
     3601 cgcgcgtgat agagcgtgtg cacggatggc gcggagagcg agacgggaat ccccgttgcc
     3661 acttcgagca ggtaacgacc gaacagcgcg gcgttgtcgg aactgccgcg cgccacgagc
     3721 acgatcaaac tgacgccgcg ctcgcgcaga aacgcgccga gacgttcaac cttcgtccgc
     3781 tccgcgtcaa tcgttcgccg caacgcctcc ggctgttcgg cgatctcttc caacatcaga
     3841 gacaacgttt catcctcctg cgcgcctgtc acaaactcgc cagatcattt cccgtcgaat
     3901 cattcaacga ctcgcgccgg acgccggaat gcaagcgtct ctgcgcgccg cgaatcatct
     3961 cataaacttt cctggcctgc gggatcattt ccagactcgg cggcgtttgc cctctcgtcg
     4021 cgcccggcaa cgcgccgaaa ttgctccagc gtccgaacgg gctggcgggg gcgaaggtga
     4081 tcgtgccgct gccgtctccg cgttcgtcga gtgagatgtc gctgagcgtg gagagactga
     4141 gcgattggac gcgtttgccg tgtttgccgg aagtgatgat gatgcggtcg ttcgtcagcc
     4201 cgtaaaaagt gtgtgtgcgc gtgcgcgcgt cggtgaagaa gcgaccgaag atcaagtgga
     4261 gtccgatgag gacgaaaggc acgccgaaga gccagaagaa gatgggcgcg ccgctcaaga
     4321 tcgccgatac ttcccagaag atggcgaagc cgccccagag caggctgaac gggatcataa
     4381 acgcgtccga gggacgcagc aggagtccct gccgtggctg cccagaccag atcaactgtt
     4441 cgcccgattc gagctgcgcg cggacggcct gcgttggatc attccacatg agtcaggttt
     4501 aagtcggatg cttcttgatc ttgcggcgtg gtttcggtaa aagtctctct gctcctttga
     4561 acagttccag acacgaatcc agatgcaaaa atcacacggc catattttaa cacggcggct
     4621 cgttagccgc cccgccttcc ttccatgcca gcacgccttc cttgcgggct ggctttgcct
     4681 cgcgctcctg ctgctcgcct cctccgtcgc ccgcgcgcaa acggacgacg cgacgcggga
     4741 tgtgcgagtt attccccaac cgcgacagtt gacgctcaaa cccgaacgct ttcgcctgac
     4801 gcgcgacgtg cgcctcgtgc tcgccgcgcc gcgctcggct gaagaccgtt tcgcggcgga
     4861 ggattttgcg gcagacgtgc gcgagacggc gggcggactc gcgctctcaa tcggcaggag
     4921 ccgccgccgc gcctcgatcc tgatcggcgc gctcgacctc gcctccgtgc agtccgcgct
     4981 ccggcgcgcg aacgtgagcg cgccgcccga cttgaacgaa gagggctatg tgctctcggt
     5041 cagcgcggat gaggtcgtcg ttgcgggtaa gacggcggcg ggaaccttct acggcttgca
     5101 gactttgaag caactcgtgc gcggcgacgc gtccgaagca ttcctgcccg gcgtcaggat
     5161 cgtcgattgg ccgcagatgc gctggcgcgc gctctctgac gacatcagcc gtggccccgt
     5221 gccgaccgtc gaatacatca agcgacagtt gcgcacgctc gccgccttca agatgaacat
     5281 gcactccttc tacatggagc acacgttcgg aagccgctcg cacccgctcg tcgcgcccgt
     5341 gggcggttcg ctcacgcccg aagagatcag agaactcttc gcctacgcgc gtcgctatca
     5401 catcgaactc gtgcccgaac agcagacctt cggacatctg cacaaggcgc tcaagttcga
     5461 gaagtacaac gaactcgccg aagtgcctta cggcgacgtg ctctcgccgc aacaggaagg
     5521 ctctttcaaa ctcgtcgccg actggtacag ggaactcgac gagttgttcc ccggaaaatt
     5581 ttttcacatc ggcgcggacg agacgtttga actcggcgag gggcagagcc gcgaacaggt
     5641 gaaggcgcgc ggcgtgggcg cagtctattt cgagcatctc cggcgcgtgc gcgaaatctt
     5701 gcagccttac aaccgccgcc tcatgttctg gggcgacatc gcgctcaatc atcccgaact
     5761 catcggcagc atcccgaaag atttgattgt gatgaactgg gcttacggag cgcgtcagga
     5821 tttcgcggcg cgcatcaagc cgttcaagga cgcaggactc gaacagttcg tctgtcccgg
     5881 cgtctccaac tggaatcagg tcttcccgca tctcgacaac gccgccaaaa acatcacggg
     5941 cttcgtgcgc gacgggcagc gcgcaggcgt gctcggcatg atgaacacga actgggacga
     6001 cgacggcgag acgctcttcg agatggcctg gtacggcgtc gtgctcggcg cggcggcttc
     6061 gtggcaggag ggcgaagtag acctgtcgca gtttgaacgc aacttcgact gggcgttctt
     6121 ccgccacgac ggcgaagagt tcgtccgcgc tctgcgcacg ctcggcagcg tcaacacgac
     6181 gctcggcatc aacacgagca atccgttgtt ctggcaagag ccgtttacga acgcgtttca
     6241 agaacgctcg cgcgggttgc gagagaagac gcgcgagatg cgccgcgccg tcgaaggcgt
     6301 ggaagagatg ctcgcgcgtg aaggggcgaa ggcgcgtcgc aaccgccagt caatcgcgtc
     6361 catgcgtttc gcggcgcaac gtctggatca catggggcga cggatgcagg tcgtcgaagg
     6421 gttcagccgc gactactggc aggcttacct gaatctaggc gaccgcaggc aggttaaccg
     6481 cctgcgacgt tatcatggag cgatctacaa caacctgcgc gagatggccg aagaactggc
     6541 gacgctcaaa gcgagctacc gcgaacaatg gctcgcggag aatcgcccct actggctcga
     6601 cagcgtgctc gcgcgctacg atcaggcgat agcgacgtgg ctcggtcgca gcaaggcttt
     6661 ggaagaagcg atgcgcgaat acaacgagag ttcgacgctt cccaaccccg aagagttcgg
     6721 cctcggctca cgccccgacc agccttaacg aacgaaagct atctttgcgc ccgacgatct
     6781 ttcttctttg cgcctgcggc acgaacccgt ccacgccgca ggcgcaaagt tgagtcttaa
     6841 cgcgccacag ggctgagaga ttcttcccgg ctttgagatc aattctcagg agttgaatga
     6901 tggacgtgcc agatgcagat caacgcggcg ggtgtggggc agagcgtctg ctttcgctcg
     6961 acgtgtttcg cggcctgacc attgcgggca tgatcctcgt caacaatccg ggaccgagcg
     7021 gcagagtcta ttggccgctc gaacacgccg cgtggcatgg ttggacgccg accgatttga
     7081 tcttcccgtt cttcctcttc atcgtcggcg tctccatcac gctcgccttc gcacgccggg
     7141 tcgaa
//
LOCUS       DASWNS010000174         9152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_369986, whole genome shotgun sequence.
ACCESSION   DASWNS010000174 DASWNS010000000
VERSION     DASWNS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369986"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..1295
                     /locus_tag="VGB76_05990"
     CDS             309..1295
                     /locus_tag="VGB76_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098487.1"
                     /translation="MILRNSRSLTTLLLLLALGVLTTSTPAQEAKPASPEEAKTDKQK
                     DVPAGTPVLWRDPGDIAARNLLHGPGGEEMKPNLTSVTFVKEETGGYSLKYRVRDGAG
                     SEWVAKIGKEAQSETAAVRLLWAIGYMTEINYLVPRLHIEGKGTFENVRLEARPEHIK
                     RLDEWKWTRNPFSGTKELRALVIMMAFLNNWDIKDTNNKVLLVPNLKTGQNRLHYIVS
                     DLGATFGRVKVDAPVIWRIKRNRNDPADYAEDPFLEDVTDGRVFLFYKGKSQRLFDDL
                     RVEEAAWLGSLLAQLSDQQIGDAFRAANYTPTEVRLMTGAVRARINELANIR"
     gene            1313..1570
                     /locus_tag="VGB76_05995"
     CDS             1313..1570
                     /locus_tag="VGB76_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098488.1"
                     /translation="MTDAQRRAICLPGAERLFEHGLPIRPGGVKVPEWAASFRAACRV
                     RTDREKSFIVPQISNPTANYTARKNFIVREHNLFTTNKVAL"
     gene            1778..2170
                     /locus_tag="VGB76_06000"
     CDS             1778..2170
                     /locus_tag="VGB76_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098489.1"
                     /translation="MKRSVFYFDDDATLLDIFQEMFEDEYDVRTTTKLAEARQMLSEC
                     ASDIIITDQSMPEISGLEFLREAIKKCPDSFRVMLTGYSLVGDVFGEIANGVIDIFIL
                     KPWKEEQMRQVLERGGAAVEVQRKLRLG"
     gene            complement(2204..3334)
                     /locus_tag="VGB76_06005"
     CDS             complement(2204..3334)
                     /locus_tag="VGB76_06005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0098490.1"
                     /translation="MSLPEGTSSPLAERLRQQIERTGAISFRDWMAAALYDEREGYYC
                     RPDRTRWGRPDSGGDYRTSPELSPLFAATFARYFAALYSELGSPVEWTILEAGAGAGD
                     FARVLLETLERTHPRVFQATRYYLAEISADARKRASETLAAFASRVEFCQNNLRGLSF
                     DAGLIFSNELLDALPVHRVRQRNGQLRELCVGLDEARNFNWIEREPTTTRLAAHFSRL
                     KTTLAEGQVAEVNLAAEDWLADAAPVIKRGYLVTVDYGAEAAELYQSTARSEGTLRGF
                     RAHQFVADLLARPGEHDLTTTINWTGLKQAGEEAGLETISLQRQDEFLLRSGFLEELE
                     RLTAQQPTEAERLRLRLGAREMILPNGMSRSFQVLVQKKKEA"
     gene            complement(3331..3978)
                     /locus_tag="VGB76_06010"
     CDS             complement(3331..3978)
                     /locus_tag="VGB76_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0098491.1"
                     /translation="MTRARFTSAPRVVKKDEKIERFLLMYCPKCGQSQVSAEVRFCSR
                     CGFPLAGVAELLLTGGMLPVHMAHNESTGSKPMSPRRRGVKQGAALLLASIFLVPFFG
                     ILHEVIGTPEEFMILGALGAMAAILRIIFAAFFEEGAPQATHFTPQPQRFYAPPQAPP
                     PHINAAVQEMLPPASQQGVPTYRQPQVDTGQLNHPPSVTDHTTRLLDKQTDPSER"
     gene            complement(4024..4512)
                     /locus_tag="VGB76_06015"
     CDS             complement(4024..4512)
                     /locus_tag="VGB76_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098492.1"
                     /translation="MNNFISTLDTQFAALHARSCALVRVLPAEFLYRQPDAAVKGRGA
                     APLYSCGEHLLRSAASVEQTFGGVTANLWDDPFEWTLPETLSTPALVAEYLAEVEATR
                     RRGLALVESDDDLLREIAVPAGERRALFALLNETLMRAAHHQGRAFATFQLFSNARLP
                     SL"
     gene            complement(4565..4918)
                     /locus_tag="VGB76_06020"
     CDS             complement(4565..4918)
                     /locus_tag="VGB76_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098493.1"
                     /translation="MRSRRKVSILAFALLGVFLFTACPSKTNIGKILDNPDRYRDKEV
                     GITGRVEDSYSVPFVGGAYKVDDGTGEIWVVSKRGGAPRKGARVGAKGHVTQGFNFGG
                     RTFGTVIEETDRRTR"
     gene            complement(5066..6571)
                     /gene="betB"
                     /locus_tag="VGB76_06025"
     CDS             complement(5066..6571)
                     /gene="betB"
                     /locus_tag="VGB76_06025"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009725.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HEY0098494.1"
                     /translation="MSSSQTASESPRRYQLFIDGKWVDAESGKTFATPNPSTGETLAE
                     VAEGDKADIDKAVAAARRAFEGKWSRMSARDRGRLLYKLAQLIESKSQELAALETADN
                     GKPIKESMYIDLPGVVENFEYFAGWATKIEGETIPVPGQMFNYTLREPLGVCGQIIPW
                     NFPLLMAAWKLAPALAAGNTIVLKPAEQTPVTALELGKLFQEAGFPDGVVNIVPGYGE
                     TAGASLASHPGIDKIAFTGSTEVGKIIARTAADNLTKVSLELGGKAPNIVFADADIDQ
                     AVNGAMMGIFFNQGQVCCAGSRLFLDERVKEEFLDRFKTKAEKIKVGDPMDKATQMGP
                     QVSAEQLTRIKGYVEIARTEGATVFTGGGAPALEGNFQKGYFFQPTVFSEVQNTMRVA
                     QEEIFGPVASVITFKDEADLIKQANDTIYGLSAGIWTRDITRAHRFAREIKAGTIWIN
                     TFNMMNAASPFGGYKQSGYGREMGKHALELYTHVKSVWVDLSGKPIGWYGN"
     gene            complement(6886..8022)
                     /locus_tag="VGB76_06030"
     CDS             complement(6886..8022)
                     /locus_tag="VGB76_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY0098495.1"
                     /translation="MTAFLFTVCGLFLLIFVAYDVYATILDASGRAGPLSGFLNRVVW
                     GASRLLAFRFSRGRRHRLLNLVGPMLMPALIVVYLLILIAGFALIYLPHMPAAFNLDP
                     PSANADWIESLYFSGTTLITIGYGDITPRTLFVRFFAIVEGASGFALISLAVTYLITV
                     YSALERKRVIALSFYHQAEEGADVAGFISHHFVSGCFHGFETVLRLAARDINELQESH
                     AEHPVIHYFHPVAVHKSMPRVFFLVLETCAVINSCLDADEYTETCGHPELRTLEASSL
                     HVLGQLTIALNLKHERMRPPEDPADVSHRLLRRYRQTVQQLTAAGIKTRQDTNEGWNL
                     YRARREQWETTLHHFATYLGYDWDEVTGDRDLRYAANESGEAKS"
     gene            complement(8019..>9152)
                     /locus_tag="VGB76_06035"
     CDS             complement(8019..>9152)
                     /locus_tag="VGB76_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY0098496.1"
                     /translation="EHDLVAASVLSGNRNFEARVHQSVKANFLMSPPLVVAFALAGRI
                     DIDMTSEPIGKGKKGEDVFLRDIWPTLEEIGEGLRAATDPEVYRRLYSDFAGQNPLWN
                     EIPSITGHVYDWDEHSTYIQEPPYFEKFGMETGRFCDIRNARPLAIFGDSVTTDHISP
                     AGAIKPTSPAGIYLQLRGVHVDDFNSYGSRRGNHQVMVRGTFANVRIKNQMVPGTEGG
                     ITCLQPVNQQMSIYDASVHYQAAGVSLVVFAGQEYGTGSSRDWAAKGTRLLGVRAVIA
                     QSFERIHRSNLIGMGVLPCQFKEGTNAKTLNLDGTETFDITGLESDQIKPRQDVTLVI
                     HRRSGETEEVPVILRIDTPIEIDYYRHGGILPFVLRQLINQAA"
BASE COUNT         1810 a         2601 c         2894 g         1847 t
ORIGIN      
        1 gaatataact atgaagggaa agactagggg aggcctcgaa ggccggggcg acacgcgtgc
       61 gaactccaca ccaacaacgt aactctcgcc ggtgatggat gatatttaat cagtgttggc
      121 aggctttgat accaatcggt acggcgaatg tgaggttgtt atgtcacatt tccctgtcaa
      181 aaccggcaga agttgtgcgg cacgcatctt gcgaaggtac cacctgtgcg gagtcacccg
      241 ggagagcctt tccttctgtt aacaggtatc ttaaattttc gacagttgaa agctgtaagg
      301 ttgaaaaaat gatattacga aacagtcgtt ctctgacaac actgctctta ctactcgcgc
      361 tcggcgtcct gacaacttcc acgcccgctc aagaagctaa acccgcatca cccgaagaag
      421 ctaagacaga caagcagaaa gatgttccgg cggggacacc tgtgctctgg cgtgatccgg
      481 gcgacatcgc cgcgcgcaac ctgctgcatg gaccgggcgg cgaggagatg aagccgaacc
      541 tgacgagcgt taccttcgtc aaggaagaga cgggcggcta ctcgctgaag tatcgcgtgc
      601 gcgacggggc gggcagcgag tgggtggcga agatcggcaa ggaggcgcag agcgagacgg
      661 cggcagtgcg tctcctctgg gctatcggtt acatgacgga gatcaactat ttagtcccgc
      721 gtctccacat cgagggcaaa ggcacgttcg agaatgtgag gctcgaagcg cgacccgaac
      781 acatcaagcg tctcgacgaa tggaagtgga ccaggaaccc cttctcaggc actaaggagt
      841 tgcgtgcgct cgtcatcatg atggcgtttc tgaacaactg ggacattaag gatacgaaca
      901 acaaagtcct gctggtgccc aacctgaaga ccgggcagaa caggttgcac tacatcgtca
      961 gcgatctcgg cgcgaccttc ggcagggtga aggtggacgc tcctgttatc tggcgcatca
     1021 agcgcaaccg caacgacccg gcggactacg ccgaagaccc cttcctcgaa gatgtgacgg
     1081 acgggcgggt cttcctcttc tacaagggca agagccagcg tctctttgat gacctgaggg
     1141 tggaagaggc ggcgtggctg ggcagcctgc ttgcgcaact gagcgatcag cagatcggcg
     1201 atgccttccg cgcggccaac tacactccga cggaagtccg tctgatgacc ggagccgtgc
     1261 gcgcgaggat caacgagtta gccaacatac gttgaagtaa tcatcacaga tattgacaga
     1321 cgctcagcgc cgcgcaattt gcttgcccgg cgctgagcgt ctgttcgagc acggcttgcc
     1381 gattcgcccc ggcggggtaa aggtgccgga gtgggcggcg tccttccgcg cggcgtgccg
     1441 ggtaagaacg gatcgagaga agagctttat tgttccccaa atctccaacc cgacggccaa
     1501 ttacacggca aggaagaact ttattgttcg cgaacataat ttgtttacga caaataaagt
     1561 tgcgctctaa aaggactaac gcttcgttgt cggggcgcgt tatactttcg tcaaaactgc
     1621 ttgagccttt tcatctggcc gcgttgactg gcttctcata cgttcgcgtt cgtggctgac
     1681 gtgttaaccg gcagttcaga aatttcgaga gagcatcacg gtgtctgttt tgcgccggag
     1741 gggcggcgtg cgccgtgggt gagcgaggtt gaggccaatg aagcggtcag tcttttattt
     1801 tgatgatgac gccacgctgc tcgacatctt tcaggagatg tttgaagacg aatatgatgt
     1861 gcgcacgacg acgaaactgg cggaggcgcg gcagatgctc tccgagtgtg cgtccgacat
     1921 cattatcacc gaccagagca tgccggaaat atcaggtctg gagtttttgc gcgaagcaat
     1981 caaaaaatgc ccggacagtt tccgcgtgat gctgaccggc tattcgctgg tgggagacgt
     2041 gttcggcgag atcgcgaacg gggtgataga tattttcatc ttgaagccct ggaaggaaga
     2101 gcagatgagg caagtgctgg aacgcggcgg cgcggcggtt gaagtgcagc gcaagttgag
     2161 gttgggttaa ccggcaacct cgaatcggag cgtcgtttcc tagtcaagcc tccttcttct
     2221 tttgcacgag cacttgaaag ctgcggctca ttccattggg gagaatcatt tcgcgcgcgc
     2281 cgagcctcag gcgcagacgt tcggcttcgg tgggttgttg tgcggtgagt cgttcgagtt
     2341 cttcgagaaa accggagcgc aaaagaaact cgtcctgacg ttggagtgaa attgtttcca
     2401 atcctgcttc ttcgcccgcc tgttttaatc ctgtccagtt aatcgtcgtc gtcaggtcgt
     2461 gctcgccggg tcgggctagc aagtctgcga cgaactggtg cgcgcgaaag ccacgcagcg
     2521 tgccctcgct tcgcgctgtc gattgataga gttctgcggc ttccgctccg taatcgaccg
     2581 tgaccaagta gccgcgcttg atgacaggcg cggcatccgc gagccaatct tccgccgcca
     2641 ggttgacttc ggcgacctga ccttcggcaa gcgttgtctt caggcgtgag aagtgtgcgg
     2701 cgagtcgcgt ggtggtcggc tctcgctcga tccagttgaa gttgcgcgct tcgtccagac
     2761 cgacgcacaa ctcgcgcagt tggccgttgc gctgtcgcac acgatgaacc gggagcgcgt
     2821 cgagcaactc attactgaaa atcaacccgg catcgaatga aaggccgcgc aagttgtttt
     2881 gacaaaactc gacgcggctg gcgaaggcgg ccaacgtttc gcttgcgcgc ttgcgcgcat
     2941 cggcactgat ctcggccagg taataacgcg tcgcctgaaa gacgcgcggg tgtgtacgct
     3001 ctaaagtttc gagcaggacg cgcgcgaaat cgcccgcgcc cgcgcccgcc tcaaggatcg
     3061 tccattcaac gggcgaacct aattccgagt agagcgcggc gaaatagcgt gcgaaggtgg
     3121 cggcgaagag cgggctgagt tcgggactgg tgcgatagtc gcccccgctg tcggggcgtc
     3181 cccagcgcgt gcggtcgggg cggcagtaat agccttcgcg ttcgtcgtag agcgcggcgg
     3241 ccatccagtc gcggaaagag atcgcccccg tgcgttcgat ctgttggcgc agacgttctg
     3301 cgagcggcga agatgtccct tcgggcagac tcatcgctcc gaaggatcgg tttgtttgtc
     3361 gagcagacgc gtggtgtggt ctgtcacgct gggcggatga ttgagttgcc ccgtgtccac
     3421 ctgcggctgg cgataggtgg gcacgccctg ttgcgacgcg ggcggcagca tctcctgcac
     3481 ggcggcgttg atgtgcggcg gcggggcttg cggcggcgcg tagaagcgtt gcggctgcgg
     3541 ggtaaagtgc gtcgcctgcg gcgcgccttc ctcaaaaaat gcggcgaaga tgattcgcag
     3601 gatggcggcc atcgcgccga gcgcgccgag tatcataaac tcctcggggg tgccaatcac
     3661 ctcgtgcagg atgccgaaaa acgggacgag gaagatgctg gcgagcagga gcgccgcgcc
     3721 ttgtttcacg ccccggcgac gcggcgacat cggcttcgag ccggtgctct cgttgtgcgc
     3781 catgtgaacg ggaagcatgc cccccgtcaa cagcagttcg gccacaccgg cgagcggaaa
     3841 gccgcagcgc gaacagaagc gcacttccgc gctcacttgc gactggccgc atttcgggca
     3901 atacatcagg agaaacctct caatcttttc gtctttcttt acgacgcgcg gcgcagacgt
     3961 gaatcgcgcg cgtgtcatag actacgccgc tcgaacctcc aaagttcgac gcggcgggag
     4021 ttttcagagc gaagggaggc gtgcgttcga gaacagttgg aacgtggcga aggcacgccc
     4081 ctgatgatgc gcggcgcgca tgagcgtctc gttcaacagc gcaaagagcg cacgcctctc
     4141 gcccgcaggc acagcgatct cccgcaacag atcatcatcc gattcgacga gcgcgaggcc
     4201 gcgtcgccgc gtcgcttcaa cctctgcaag atactccgcg acgagggcgg gcgtcgagag
     4261 cgtctccggc agcgtccact cgaaagggtc gtcccacaag ttagccgtga cgccgccgaa
     4321 cgtctgttcg accgacgccg cgctgcgcaa gagatgttcg ccgcacgaat agaggggagc
     4381 cgcgcccctt cccttcaccg ccgcgtccgg ctgccggtag agaaactctg cgggcagcac
     4441 gcgcacgagc gcgcacgaac gcgcatggag agcggcaaac tgtgtgtcga gagttgaaat
     4501 gaagttgttc attgattcat ttcttcattg cttcgatgga tcaatgagcc aatgaacggg
     4561 tgcgttatct ggtgcgccgg tctgtctctt cgatcacggt gccgaaggtg cgaccgccga
     4621 aattgaagcc ctgcgtgacg tgacctttcg cgccgacccg cgctcccttc cggggcgcgc
     4681 cgccgcgctt tgagacgacc cagatttcgc ccgttccgtc gtcaaccttg taagccccgc
     4741 cgacgaaagg cacgctgtag ctatcttcca ccctcccggt gatcccgacc tccttgtcgc
     4801 gataacgatc cgggttgtcg agaatttttc cgatgttggt cttactcgga caggcggtga
     4861 acaggaaaac accgaggagg gcgaaagcga gaatagaaac ttttcgacgt gagcgcatgg
     4921 cttctccttc ttatgttgaa atacgggaga ggcggaagag agggcggagg gatgagggat
     4981 gagggatgaa tcagaaagga agtagactca acttagttga gcaatttctg attcatccct
     5041 catccttcat ccttcatccc ttcccttagt tgccgtacca gccgatgggt ttgcctgaga
     5101 gatcaaccca gacgctcttg acgtgcgtgt agagttcgag cgcgtgcttg cccatctcgc
     5161 gcccgtagcc ggattgcttg tagccgccga agggcgaggc ggcgttcatc atgttgaagg
     5221 tgttgatcca gatcgtgccc gctttgatct ctctggcgaa gcggtgtgcg cgcgtgatgt
     5281 cgcgcgtcca gatgcctgcg gagaggccgt agatcgtgtc gttggcctgc ttgatgagat
     5341 cggcctcatc cttgaacgtg atgacggagg cgacgggtcc gaaaatctct tcctgtgcga
     5401 cgcgcatcgt gttctgcact tcgctgaaga cggtcggctg gaagaagtag cctttctgga
     5461 agttgccttc gagcgcgggc gcgccgccgc ccgtgaagac ggtcgcgcct tcggtgcgcg
     5521 cgatctccac gtagcccttg atgcgcgtga gttgttcggc ggaaacctgc ggtcccatct
     5581 gcgtcgcttt gtccatcggg tcgccgacct tgatcttctc cgctttcgtc ttgaaccgat
     5641 ccagaaactc ctccttcacg cgctcgtcaa ggaagagacg cgaaccggcg cagcagacct
     5701 gcccctgatt gaagaagatg cccatcatcg cgccgttgac cgcctgatcg atgtcggcgt
     5761 cggcgaagac gatgttcggg gctttgccgc cgagttcgag cgagactttg gtgagattgt
     5821 cagccgccgt gcgcgcgatg atcttgccca cttcggtcga gccggtgaag gcgatcttgt
     5881 ctatgccggg atgcgaggcg agcgacgcgc ccgccgtctc gccataaccg ggcacgatgt
     5941 tgacgacgcc atcggggaag cccgcttcct gaaacagctt gccgagttcc agcgccgtca
     6001 ccggagtctg ttcggcgggt ttcaggacga tggtgttgcc tgccgcgagc gcgggcgcga
     6061 gtttccaggc ggccatcagg agcggaaagt tccaggggat gatctggccg cagacgccga
     6121 gcggctcgcg cagggtgtag ttgaacatct ggccggggac ggggatcgtc tcgccttcga
     6181 tcttcgtcgc ccacccggcg aagtattcga aattctccac cacgcccggc agatcaatgt
     6241 acatggactc cttgatcggc ttgccgttgt cggcggtttc cagcgcggcg agttcctgcg
     6301 atttcgactc gatcaattgc gcgagtttat acagcagacg cccgcgatca cgcgcgctca
     6361 tacgcgacca cttaccctcg aacgcgcgcc gcgcggcagc gacggccttg tctatatcgg
     6421 ccttgtcgcc ctctgcgacc tcggcgagcg tctcgcccgt agaagggttc ggcgtggcga
     6481 aagtcttgcc ggattcggca tcaacccact tgccgtctat gaagagttga tagcgacgag
     6541 gcgactcgga cgcagtttga gatgacgaca tggaaaactc cttagctcag attcttaaaa
     6601 atatgttgga gagtcttgac agtgaacttc gtgcacggtc attactttag catctactcc
     6661 gggcgaagtc taaacgcccc ccttccgtct cacgctctcg ctttggcggc agacgcccgc
     6721 cgcaaaaccg aaggggcagc cgtccttgcc caaggcggct gcccctctct gcgctccggt
     6781 gcgatgggga tcagagttgt gagaaaaatt ttcgggcggg tcgaactcta caaggcgatt
     6841 cggggtgaag gtgtggggtc ggggaaacgg agccgggttg taggttcagg acttcgcttc
     6901 gcccgactcg ttggcggcgt agcgcaggtc gcggtcgccc gtcacttcgt cccaatcgta
     6961 gccgagatag gtcgcgaagt ggtgtagtgt cgtctcccat tgctcgcgcc gcgcgcggta
     7021 gaggttccag ccttcgttcg tgtcctggcg cgtcttgatg cccgccgccg ttagttgttg
     7081 caccgtctgg cggtagcgtc tgagcagacg atgggaaacg tctgcggggt cttccggcgg
     7141 gcgcatgcgt tcatgtttca gattgagcgc gatggtgagt tggccgagca cgtggaggct
     7201 gcttgcttcg agcgtgcgca gttccggatg cccgcacgtc tccgtgtatt cgtctgcgtc
     7261 gagacacgag ttgatgacgg cgcaggtttc cagcacgagg aagaagacgc gcggcatgct
     7321 cttatgcacg gcgacgggat gaaaatagtg tatgacggga tgctcggcgt gcgactcctg
     7381 aagttcgttg atgtcgcgcg ccgccaggcg caggacagtc tcgaagccgt gaaagcatcc
     7441 tgagacaaag tggtgcgaga tgaacccggc cacgtccgcg ccctcttccg cttgatgata
     7501 aaaggagagg gcgatcacgc gctttcgttc gagcgcgctg tagaccgtga tgagatacgt
     7561 cactgcgagc gagatcaggg cgaacccgct cgcgccttcc acgatggcaa agaagcgcac
     7621 aaagagcgtg cgcggcgtga tgtcgccgta gccgatggtg atcagcgtgg tgccgctgaa
     7681 gtagagcgat tcgatccagt cggcgttcgc agatggaggg tcgaggttga aagcggcggg
     7741 catgtgcggg aggtagatta aagcgaagcc cgcgatgagg atgaggagat agacgacgat
     7801 gagcgcgggc atcagcatcg gcccgacgag attgagcagg cgatgccgac gcccgcgcga
     7861 gaagcgaaag gccagcaggc gcgacgcgcc ccacacgacg cgattcaaga atccgctcaa
     7921 cggcccggcg cgcccgctcg catcgaggat cgtggcgtac acgtcgtagg ccacgaagat
     7981 gagcaggaac aggccgcaga cggtgaacaa aaaagccgtc acgctgcttg attgatgagt
     8041 tgacgcaaca cgaagggcag gatgcctccg tggcggtagt agtcaatctc aatcggcgta
     8101 tcgatgcgca ggatgacggg cacttcttcc gtctcgcccg accggcgatg tatcacgagc
     8161 gtcacgtcct gtcgcggttt gatctgatcg ctttcgagtc cggtgatgtc gaaagtctcc
     8221 gtgccgtcga gattaagcgt cttggcgttg gtgccctctt tgaactggca gggcaagacg
     8281 cccatgccga taagattgct gcgatggatg cgctcgaaac tctgcgcgat gacggcgcgc
     8341 acgcctaaca ggcgcgtgcc cttcgcagcc caatcgcgcg aactgcccgt gccgtactcc
     8401 tgcccggcga agacgacgag cgaaacgccc gccgcctgat aatggacgga ggcatcgtaa
     8461 atgctcatct gctggttgac gggttgcagg caggtgatgc cgccctccgt gccgggaacc
     8521 atctgatttt tgatgcgcac gttggcaaac gtgccgcgca ccatcacttg atgattgccg
     8581 cgccgcgagc cgtaggaatt gaaatcgtca acgtggacgc cgcgcagttg caggtagatg
     8641 cccgccggag aagtcggctt gatcgcgccc gccgggctga tgtggtcggt cgtcaccgag
     8701 tcgccgaaga tggcgagcgg gcgcgcgttc ctgatgtcgc agaacctgcc cgtctccatg
     8761 ccgaacttct cgaagtaggg cggctcctga atgtaagtcg aatgctcgtc ccaatcgtag
     8821 acgtggccgg tgatggaagg gatttcgttc cagagcggat tctgcccggc gaagtcgctg
     8881 tagagacggc gatagacttc cgggtcggtc gccgcgcgca agccttcgcc gatctcttcg
     8941 agcgtcggcc agatgtcgcg caggaacacg tcctcgccct tcttcccttt cccgataggc
     9001 tcgctcgtca tatcgatatc gatgcgaccg gcgagcgcga aggccacgac gagcggcgga
     9061 ctcatcagaa agttcgcctt aacgctctga tgcacacgcg cctcgaaatt cctgttgccc
     9121 gacaaaacgc tcgccgccac gagatcgtgt tc
//
LOCUS       DASWNS010000175         5269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_448263, whole genome shotgun sequence.
ACCESSION   DASWNS010000175 DASWNS010000000
VERSION     DASWNS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448263"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VGB76_06040"
     CDS             <1..953
                     /locus_tag="VGB76_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY0098497.1"
                     /translation="QASEESAVGISRDARVLWVEEDGEVTANTTQWNPPSWGLDRIDQ
                     KFLPLSSSYTYTRTGAGVNGYVIDTGILFSHTDFTTPFPRAYIGTSTVGGNGIDCNGH
                     GTHVAGTMAGTKYGVAKGLRFWGVQVLNCAGSGTVASVIAGVNWVRWHRLQPPFINSP
                     AVANMSLGGPYSAALNAAVVNAINAKITFAVAAGNNNANACLSSPASVGPAITVGATD
                     KWDFRSWFSNWGPCLDLFAPGEQITSAWIGWPANNATATISGTSMASPHAAGVAAQYL
                     QMVPWATPAQVSAALNANATAGVVINPGPGSPNRLLFTNY"
     gene            complement(1723..2814)
                     /locus_tag="VGB76_06045"
     CDS             complement(1723..2814)
                     /locus_tag="VGB76_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098498.1"
                     /translation="MKQQVQLATLAAILWCAPHLAAQQPAPATPTPETAPAAVKTDQP
                     QKQVPAVGDTQAAPVAKAEVECYCESQALPEVLAEVGSDKITAREIDEKIKDQVRDLK
                     QQVVQTRARELYIQINTRLLEAEAKRRGKTTKALIEEEIVAKLKEPTEAEAQTFYDQN
                     KDRIRQTFPTVKTQIIEHLRVAGQRDEAKKFADRLRAAAQVVINEKDAPKPGQPLVPT
                     LVLATVNGAPITVGDIEDTLRPLIFSVQTELYKLRKQVIDVKVNDLLLNREAQARKMT
                     PAALVETEATGKAKKVTDADARAFYEERKQEIGGEFESQKQQLLLFLQEQEERRAQAA
                     FAERLRSATAPKIFLREPEQSTSKSSPYP"
     gene            complement(2926..4593)
                     /locus_tag="VGB76_06050"
     CDS             complement(2926..4593)
                     /locus_tag="VGB76_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0098499.1"
                     /translation="MSFRSIKLFIILFAVLAVVWLGLDGRGRAAALSTPDGLTPAEQR
                     GKTIYLKGEDAGGEITALLGGSDMEVPATTFACASCHGLKGEGTSEGGLQTPALVWSA
                     LAAPATSALTRQERAAYTEATLVRALRVGLDSSRGRLHPGMPRYRMSDEQAANLVAYL
                     KKLGHEVEPGVSETTIKVGAALPLSGALAQVGQDLKATLSASFAEANERGDTYGRRFE
                     LVVEDSRGDIEGTREAHRKLIEQANVFALAGSYEPRGSVAADEFLRQSEVPLVGPVTL
                     SPHLTLPPNRFVFYLLPTFDQQARSLVDFIAAQPFYGGAAVESATMKDGQTAPAARKA
                     RVAVIYVNGQLETGALAALRAQARLRSLEIVSEQSYESGRFAPTRTVEMLARTRPEYV
                     FFFGGAQEIVACARELERAQVNASLVSSVMMLGGGAFELPPSVAARTFLAYPSALPNQ
                     EDFAEFLSVMQRRGVALRSPAFQSIAYAAAKTLIEATKLSTRKLSRHTLVNSLEGLRA
                     YRTGVVPPLSFGPNRRVGVEGSYVVGIDLVNKRYTALSEQIIPRDSK"
     gene            complement(4602..>5269)
                     /locus_tag="VGB76_06055"
     CDS             complement(4602..>5269)
                     /locus_tag="VGB76_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0098500.1"
                     /translation="SATKTVAGIVTDAADKDSSRPTARGDATPDPAPANNATAAAADG
                     TVPANKAASTKPSADYFPNHLLVTQDNKPVRFYNDLLRDKVVLINFMFTTCQGVCSPM
                     TANLLKVQQYLGDRVGRQINMITITVDPATDTVRVLKDYTEKFKIKPGWYFLTGKKEN
                     VDWVLYKVGGYVKNRAEHTSMLVMGNDATGEWVKMPAMASAADIANAAVKLADSKADS
                     KND"
BASE COUNT          969 a         1603 c         1583 g         1114 t
ORIGIN      
        1 tccaagcgtc ggaggagtcc gccgtgggta taagccgtga tgcgcgcgtc ctgtgggtcg
       61 aagaggacgg ggaagtcaca gccaacacga cgcagtggaa ccctccgtca tgggggcttg
      121 accgcatcga ccagaaattc ctgccgctca gcagttccta cacctacacc agaacggggg
      181 ctggcgttaa cggctatgtc atcgataccg gcatcctctt ctcgcacacc gacttcacca
      241 ctcccttccc gcgcgcctac atcggcacct ccacggtcgg cggcaacggc atcgactgca
      301 acggccacgg cacgcacgtc gcaggcacga tggccggcac caagtacggg gtggcgaaag
      361 gcctccgatt ctggggcgtc caagtgctca actgcgcagg cagtggcact gtcgccagcg
      421 tcatcgccgg cgtcaactgg gtgagatggc accgcctcca gccgccgttc atcaattccc
      481 cagcggtcgc caacatgagc ctcggcggcc cgtacagcgc ggcgctgaac gccgccgtcg
      541 tcaacgccat caacgccaag atcactttcg ccgtggccgc cggcaacaac aacgccaatg
      601 cgtgtctctc ctctcccgcg agcgttgggc ctgcgattac ggtcggcgca accgataagt
      661 gggacttccg ctcctggttc tcgaactggg gtccctgcct cgacctattc gcgccgggcg
      721 agcagatcac ttcggcttgg atcgggtggc cggcgaacaa tgcgacggcg accatctccg
      781 gcacgtcgat ggcgtcgccg cacgcggctg gcgtggcggc gcaatacttg caaatggtgc
      841 cgtgggcgac gcccgcgcag gtcagtgccg cgctcaatgc gaatgcaacg gccggcgtgg
      901 ttattaaccc cgggccgggg tcgccgaacc gcctgctctt cacgaattac tgacgacggc
      961 gtcaaaactg tcgaactccc atgaggtcat cggcagacga gcaccacaca ggtgctcgtc
     1021 tgccgatgca gtcaggaaca gtgttgcgaa aaggtagtgt tctatgaaag gggcgtgtca
     1081 agaaaaaact tctcccctgc tgacgatcaa gagctatttc tttgaccgct agtcctcaac
     1141 tgacccgcaa gccacgtcag cgtggcctag tggctgaact gcccgctaat gctcgtcagg
     1201 ggacgggctt tatgtcagcc gttgacttct gacggctttc cccgcggcga aagtcccatg
     1261 gtagcgtcgg cgaggtctga aaccagaaat ctagctgcag tttgctcacc cgcccctcct
     1321 cgaagctata cacatcccga tcctgcgcgg gctggtcacg ctcgtagtac ttgtaatgcc
     1381 aagccagccc cgcccgcagc atttcccggt taacgtccaa cctctaagat tcttattaat
     1441 ttggagctat atcatccgct caattggtag caaataggct aaggcgaaag ggttcaagcc
     1501 gatctccgtt gtgaacacta caatttcaga tagtgttgca aaaaatattt tagccacaat
     1561 tactagaggt aagaaggttg ccttggcctc aatatcttgt gtgcctagta agtttttgca
     1621 acactaccgc gaagggtggc aggtacggct cacgagacca cctcgtgctg ctgatgacgt
     1681 atcgtcacgg gctgagagtc tcggaactga tcgacatcag gctcaaggat agggagagct
     1741 tttcgaggtg gattgttccg gctcgcgtaa aaagattttg ggcgcggtgg cactgcgcag
     1801 tcgctccgcg aaagcggcct gtgcacgccg ttcctcctgc tcctgcaaga agagcaacaa
     1861 ctgctgcttc tgcgactcga actctccgcc gatctcctgt ttgcgctctt cgtaaaatgc
     1921 gcgtgcgtcc gcgtccgtca cctttttcgc tttgcccgtc gcttcggttt caaccagcgc
     1981 ggctggcgtc atcttacgcg cctgcgcctc gcggttcagc agcagatcgt tgaccttcac
     2041 atctatgacc tgcttgcgca gcttgtagag ttccgtctgc acgctgaaaa tgagagggcg
     2101 cagggtgtct tcgatgtcgc ccaccgtgat gggcgcgccg ttgaccgtgg cgaggacgag
     2161 cgtgggcaca agaggctgac ccggtttggg agcatccttc tcgttgatga ccacctgcgc
     2221 ggcagcccgc agccggtcgg caaacttctt tgcctcgtca cgctgtccgg cgacgcgcag
     2281 gtgctcgata atttgcgtct tgacggtcgg gaaagtctga cgaatgcgat ccttgttctg
     2341 gtcgtagaaa gtctgcgctt cggcttccgt cggttccttt agtttggcga cgatctcttc
     2401 ttcgatgagt gccttggtgg ttttaccgcg acgcttggct tccgcttcca gcagtctcgt
     2461 attgatctga atgtagagtt cgcgcgcgcg tgtctggacg acctgctgct tcaaatcgcg
     2521 tacctgatct ttgatcttct cgtcgatctc tctggcagtg atcttgtcgc tgcccacctc
     2581 tgcgagcact tcgggcaacg cctgcgactc gcaatagcac tcgacctccg ctttcgctac
     2641 gggcgcagcc tgcgtgtcgc cgaccgccgg gacttgcttt tgaggttgat cagttttaac
     2701 cgctgccgga gcagtctccg gcgtgggtgt ggcgggcgcg ggttgctgtg cggcgaggtg
     2761 gggcgcgcac cacaggatgg cggcgagcgt tgccagttgt acttgctgtt tcataaggct
     2821 ccgataaaag tgcgtttctt gattgtgaac ctgcgacgtt gattcgttcg agttgacgcc
     2881 gccggcatga aattattgtc gtcggactta atgcagtcgc gccgtttatt tgctatcgcg
     2941 cgggatgatc tgctcactta aagcggtata tcgcttgttg accagatcga tcccgaccac
     3001 gtaagaacct tcaacgccga cgcggcggtt cgggccgaaa ctgagtggcg ggacgactcc
     3061 ggtgcgatag gcgcgtaacc cttcgaggga atttacaagc gtgtggcggc taagctttct
     3121 ggtgctcaac ttcgttgctt cgatcaaggt cttcgcagcc gcgtaggcga tgctctgaaa
     3181 agcagggctg cggagagcga cgccgcgccg ttgcatgacg gagagaaact ccgcgaagtc
     3241 ttcctgattc ggcaacgccg acgggtacgc caggaaggtg cgggcggcga cggacggcgg
     3301 cagttcaaaa gctcccccgc cgagcatcat cacggaacta acgagcgacg cgttgacctg
     3361 cgcccgctcc aactcacgcg cgcacgcgac gatctcctgc gcgccgccga agaagaagac
     3421 gtattcgggt cgggtgcgcg ccagcatctc gaccgtgcgg gtgggggcga agcgacccga
     3481 ctcgtagctt tgttcagaca cgatctccaa cgaacgcaga cgcgcctgcg cccgcagggc
     3541 agccagcgca cctgtttcga gttggccatt gacatagatg acggcgactc ttgccttccg
     3601 cgcggcgggc gcggtctgcc cgtccttcat agtggcagac tcgacggctg ccccgccgta
     3661 gaagggctgc gcggcgatga aatccacgag tgagcgcgct tgctggtcaa acgtgggcaa
     3721 caggtaaaag acgaagcgat taggagggag cgtgagatgc ggcgagagcg tcacgggacc
     3781 gaccagcgga acttcgctct gccgtagaaa ctcgtctgcc gccacgctgc ccctcggctc
     3841 gtaacttccc gccagcgcga agacattcgc ttgctcgatc aatttgcggt gcgcctcgcg
     3901 cgttccttcg atgtcgccgc gcgagtcttc aaccaccaac tcgaagcgtc gcccgtaggt
     3961 gtcgccccgt tcgttggcct ccgcgaacga ggcggagagc gtcgccttga ggtcttgacc
     4021 cacttgcgcg agcgcgccgg acaaaggcag agccgcgccc accttgatcg tcgtctcgct
     4081 cactcccggt tcaacttcgt ggcctaactt tttgagatag gcgacgagat tcgccgcctg
     4141 ctcgtcgctc atgcgataac ggggcatccc cgggtgcaga cgcccgcgcg aagaatcgag
     4201 gccgacgcgc agagcgcgca cgagggtggc ctccgtgtaa gcggcgcgct cctgcctcgt
     4261 gagagcggaa gtggccggag ccgccagcgc agaccagacg agcgcgggag tttgcaagcc
     4321 gccttcactt gtgccttcgc ccttcaggcc atgacagctg gcgcaggcga atgtcgtggc
     4381 gggcacttcc atatcgctgc cgccgagtaa ggccgtgatc tccccaccgg catcttcacc
     4441 tttcagataa atcgtcttgc cgcgctgttc cgcaggcgtc aacccgtcag gtgttgacaa
     4501 cgccgcagcc ctgccgcgac cgtcaagccc gagccagact accgctaaca cggcaaagag
     4561 gatgatgaag agtttgatgg aacgaaagct catcacacgg gttaatcgtt cttcgagtca
     4621 gcctttgagt cggccagttt gacggcggcg ttagcgatgt ccgcagcgct ggccatcgcg
     4681 ggcatcttca cccattcgcc ggtcgcatcg ttgcccatca cgagcatgct ggtgtgctcc
     4741 gccctgttct tcacatagcc gccgactttg tagagcaccc aatcaacgtt ctcttttttt
     4801 ccggtcagga aataccagcc cggtttgatc ttgaacttct ccgtgtagtc cttgagcacg
     4861 cggacggtat ccgtcgccgg atcgaccgtg atcgtgatca tattgatctg gcgaccgacg
     4921 cggtcgccga gatactgctg tactttgagc aggttggccg tcatcggcga acagacgccc
     4981 tgacaggtgg tgaacataaa gttgatgagt acgactttat ctctgagcag gtcgttgtag
     5041 aaacggacgg gtttgttgtc ctgcgtgacg agcaggtggt tgggaaagta atccgccgac
     5101 ggtttcgttg acgcggcctt gttggcgggc actgtcccgt cggcggcggc ggcggtcgca
     5161 ttgttggcgg gtgctgggtc gggcgttgca tcgccccgcg ccgtcggcct gctgctgtcc
     5221 ttatcagcgg cgtcggtcac gatccccgcg acggtcttgg tcgcagaga
//
LOCUS       DASWNS010000176         8771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_241050, whole genome shotgun sequence.
ACCESSION   DASWNS010000176 DASWNS010000000
VERSION     DASWNS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241050"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VGB76_06060"
     CDS             <1..1038
                     /locus_tag="VGB76_06060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0098501.1"
                     /translation="IAGRNMLAVQFFNAVLGAATAPVVYICTQRIFQNVRAARLAALL
                     VAFYPSLVLWSSQALKDAPIVFLLTVTTLATLKLGEKFNVKYLVALLFSLFGLLSLRF
                     YVFYIMIVAVGAGFIIGMRQLTAQSFARQLLLVILVGLSLTYLGVLRTASEQFEDFGN
                     LERVQRSRQDLASSAQSGFAQDVDVSTTSGALSVIPVGMVYLLFAPFPWQMVNLRQSI
                     TLPEMMLWWASFPLLVLGGWFTLKYRLRQALPILLFTTMLTLAYSIFQGNVGTAYRQR
                     SQLLIFYFIFVAVGFVLLRERREDRLLQALAARKLEINKRRQTVAPNVTEGAGGGVAA
                     TGQAQSVAKRV"
     gene            1042..2928
                     /gene="asnB"
                     /locus_tag="VGB76_06065"
     CDS             1042..2928
                     /gene="asnB"
                     /locus_tag="VGB76_06065"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY0098502.1"
                     /translation="MCGIVGIVDRRRREPVDEVVLGRMCEAIRHRGPDEDGFFRGRGV
                     GLAMRRLAIIDLKSGQQPMYNTDRTAAIVFNGEIYNYKEVRAALEARGHTFQTNCDTE
                     VIVHAYDEYGADCPKHLHGMFAFAIWDERREELFLARDRVGKKPLLYAHVGSQLIFGS
                     EFSALLLHPEVSREINREAIHHYLTFMCVPAPLTAYRDIRKLEPGHSLRFTREGEIKL
                     ERYWQPDFSRKTKMSEEKAGERAVEILREAVRVRLMSEVPLGAFLSGGVDSSAVVALM
                     SEVSSTPVKTFSIGFEEQDFSELHHARRVAEHVGAEHHEFIVRPDAMEVLPTLVEHYG
                     EPYADSSAIPTYYVSRETRRHVTVALNGDGGDECFAGYERYAAMKLAGKYRNVPGLLR
                     EGLIKNAVALYPTSETRRSRARDLKRFLDAASLPTVERYMRWISVLDQTAKDDLYTAD
                     FRREMEGHEASDFVAPWFAQANGAGLVDASLLADTMTYLPNDLLVKVDIASMAVSLEA
                     RSPFLDHHVIEFAASLPENLKLRGLTTKYLLKRVLAKLLPAENLSRRKMGFGVPIGHW
                     LRGEMQPFLRETLLSEKALGRGFFRPEAVTRMVELHTRGERDYAHQLWTLLMLELWFQ
                     RFID"
     gene            2956..3708
                     /locus_tag="VGB76_06070"
     CDS             2956..3708
                     /locus_tag="VGB76_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0098503.1"
                     /translation="MKGVVLAGGLGSRLMPLTAVTNKHLLPVYNQPMVYYPIQTLVNA
                     GIRDIMIVTGGNSAGDFLKLLKNGKAFGLKHLNYTYQEGEGGIAAALALCEDFADGEP
                     VCIVLGDNIIEGNICQAVRAYRHQGGGAKILLKQVSDPQRFGVPELEGSRVVRIEEKP
                     KEPKSDYAVIGVYMYDAEVFQIIKTLKPSERNELEITDVNNAYIARGEMTWNELEGWW
                     TDAGTFESLLHAGTLVAETGANKMEAVNANHA"
     gene            3701..4207
                     /locus_tag="VGB76_06075"
     CDS             3701..4207
                     /locus_tag="VGB76_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEY0098504.1"
                     /translation="MPEREPQGSAPFKRGQIRDVVVRDVRKFVDERGWLAEVFRHDEM
                     DAEYFPTMSYISSTEPGVQRGPHEHVDQADLFVFIGPANFKLRLWDNRRDSETYNHVM
                     TLYVGADNPKSVLVPKGVVHAYRNVGSTAGIVINCPNRLYMGEGKRAEIDEIRHEDDP
                     ATIYRMED"
     gene            4297..5718
                     /locus_tag="VGB76_06080"
     CDS             4297..5718
                     /locus_tag="VGB76_06080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0098505.1"
                     /translation="MKVVRIIARLNVGGPAKHVAWLTAATRRAGVESELVAGTVPPGE
                     DDMSYFAAAQGVEPIIVAEMSREVSPKDALTVWKLYRLFRRLQPDIVHTHTAKAGTVG
                     RVAGVLYRWLTPAALAGRPRPCRFVHTYHGHIFHSYYGRLKTLVFLTVERVLARTATD
                     RIVVISRQQFREIHEQFGVGRARQFRVIPLGLDVEAFAGWRARREAARAALGAKEDEL
                     LVGIVGRLTEIKNHKLFLEVAALYLERDAAAQATGEVGETFGAAASRRVRFVVVGDGH
                     LREALQTQADALGIGERVTFTGLRDDPENFYAALDIVALTSLNEGTPLTLIEAMANAR
                     PVVATAVGGVVDLLGEASDDEPAAHATAPAHAYTICERGVRVGTNDAEAFCAALRRLI
                     TDEELRRTLGERGRAFVAEHYSKERLVADVLNLYSELLPSQTSPTAERVSSHARGKGA
                     ATAKTAAKQGSVPGIETARLKGD"
     gene            5722..6684
                     /locus_tag="VGB76_06085"
     CDS             5722..6684
                     /locus_tag="VGB76_06085"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0098506.1"
                     /translation="MRVLITGGAGFIGSHLSDAYLERGDEVYCIDDLSTGSFENIAHL
                     KTHPRFHYTIDSVHNVPVCAELVDQCDVVFHLAAAVGVKLIVESPVRTIETNVRGTEV
                     ILSLANKKKKKVLVASTSEVYGLSDQVPFREDGNLVMGATTKGRWSYACSKAIDEFLA
                     LAYWREKKLPTIVVRLFNTVGPRQTGQYGMVIPTFVRQSLAGRPITVYGNGKQTRCFG
                     YVGDVVGALMKLMDAPEAVGQVFNIGSNEEVSILEVAERVKELTDSKSEIVFMPYDEA
                     YEEGFEDMPRRVPDISKVSALIGFKPTEKLDGILQKVIAHHSGQ"
     gene            6874..8640
                     /locus_tag="VGB76_06090"
     CDS             6874..8640
                     /locus_tag="VGB76_06090"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098507.1"
                     /translation="MSFALALGLTPLVRRLARHWGMVAQPKADRWHNKPTAMMGGLAI
                     FLTVVIAYLIFNAQMPHHPYGWVVILGSTCLFVVGLVDDILHIKPYQKLIGQVMGASL
                     IVYYGLSLPWSGSPSLNMAITIFWLIGITNALNLLDNMDGLAAGIAAIAAGFLSASFL
                     SNGRPAEALLLGVFCAALVGFLAYNSNPASIFMGDCGSMFIGFFLASTALMSAAGARS
                     RSFLVVLAVPVLILLIPIFDTTFVTILRKLSGRSASQGGRDHTSHRLVALGMTERRAV
                     WMLYAFAGLSGVLALLVSRVKFEVSVAVIVAFTLVLALLGVYLAGVKVYEEEEVMRAR
                     EKPLVAFFIDLSYKRRIFEVMLDVVLIILSYYSAYTLLFGAMTETGAWNLFLRTVPVL
                     VCVKMFAFLAAGVYRGLWRYISIDGVVVFAKAVVLGAVGSVLALLVAFRFEGLSRAVF
                     VLDTMMLFMMLAGSRFAFRLFRHALPVAEASGARRVLIYGAGDGGELLLREVLNNREL
                     QLTPVGFVDDDPLKKGKVIHGLRVFGGNGALKSICEQQRIAEVLISSTQISEERLATI
                     LQDCEAAQVTLKRMRIQIDQLN"
BASE COUNT         1645 a         2616 c         2751 g         1759 t
ORIGIN      
        1 atcgccgggc gcaacatgct ggccgtgcag ttcttcaacg ccgtgctcgg agccgccacc
       61 gcgcccgtcg tctatatctg cacgcagcgc atctttcaga acgtgagagc cgcgcgcctc
      121 gccgcgctcc tcgtggcctt ctacccgtcg ctcgtcctct ggtcgtcgca agccttgaag
      181 gacgcgccca tcgtgttcct gttaacggtg acgactttag ccacgctcaa actcggcgag
      241 aagttcaatg tgaagtacct cgtcgcgctt ttgttctcgc tcttcggcct gctcagcctg
      301 cgcttctacg tcttctatat catgatcgtc gcggtgggag cgggctttat tatcggcatg
      361 agacaattga ccgcgcagag tttcgcgcgc caactgctcc tcgtcatcct cgtcggactg
      421 tcgctgacct atctgggcgt gctgcgcacg gcgagcgagc agttcgaaga cttcggcaat
      481 ctcgaaaggg tgcaacggag ccgacaggac ttggcctctt cggcacaatc gggcttcgcg
      541 caggacgtag atgtctcgac cacttccggc gcactcagcg tgatccccgt cggcatggtc
      601 tatctcttgt tcgcgccctt tccgtggcag atggtcaact tgcgccagag catcactttg
      661 ccggagatga tgttgtggtg ggcgagcttt ccgctgctgg tcttgggcgg gtggttcacg
      721 ttgaagtacc gtctgcggca ggcgttgccg attctgttgt tcacgacgat gctgactttg
      781 gcctattcga tctttcaggg gaacgtgggg acggcctaca ggcagcgttc gcaactgctc
      841 atcttctatt tcatcttcgt cgccgtcggg ttcgtcctgc tcagggagcg gcgcgaggat
      901 cgactcctgc aagctctggc ggcgcgcaaa cttgagatca ataagcgtcg gcagacggtt
      961 gcgccgaacg tgacggaagg ggcgggtggc ggcgtcgccg ccacggggca ggcgcagagc
     1021 gtggcaaaga gggtttaagt tatgtgcggc attgtaggga ttgtagatcg caggcggcgc
     1081 gagccggtgg acgaagtggt gctcgggcgg atgtgcgagg ccattcgcca tcgcggcccg
     1141 gacgaagacg gtttttttcg cggcaggggc gtcggcctcg ccatgcgccg tctcgccatc
     1201 atcgatctca agagcggcca gcagccgatg tacaacaccg accgcaccgc tgccatcgtc
     1261 ttcaacggcg aaatctacaa ctacaaagag gtgcgcgccg cactggaagc gcgcgggcac
     1321 acttttcaaa ccaactgcga cacggaagtg atcgtccacg cttacgacga atacggcgcg
     1381 gactgtccga agcacctgca cggcatgttc gccttcgcca tctgggacga gcgacgcgaa
     1441 gagttgtttt tggcgcgcga ccgcgtcggc aaaaagcctc tgctctacgc gcacgtgggc
     1501 agccaactca tcttcggttc ggagttcagc gcgctcctgc tgcacccgga ggtgagccgc
     1561 gagatcaacc gcgaggccat tcatcactat ctcactttca tgtgcgtgcc cgcgccgctc
     1621 accgcctacc gcgacatacg caaactcgaa cccggccact ccttacgctt cacgcgcgaa
     1681 ggcgagatca aacttgagcg ttactggcaa ccagactttt cccgcaagac gaagatgagc
     1741 gaggagaagg cgggcgagcg cgccgtggag attttgcgcg aggccgtgcg cgtgcgtttg
     1801 atgtcggagg tgccgctcgg cgcgtttctc tccggcggcg tggattcgtc tgcggtcgtc
     1861 gcgctgatga gcgaagtttc gagcacgccg gtcaagactt tctccatcgg tttcgaggaa
     1921 caggacttca gcgaactgca tcacgcgcgc cgcgtcgccg aacacgtcgg cgcggagcat
     1981 cacgagttca tcgtgaggcc ggacgcgatg gaagttctgc ccacgctcgt cgaacactac
     2041 ggcgagcctt acgccgattc ctccgccatc ccgacctatt acgtttcacg cgagacgcgc
     2101 cgccacgtca cggtcgctct caacggcgac ggcggcgacg agtgcttcgc gggctacgaa
     2161 cgctacgccg cgatgaaact cgcgggcaag tatcgcaacg tccccggcct gctgcgcgaa
     2221 ggcttgatca aaaacgcggt cgcgctctac cccacttcgg agacgcgccg cagccgcgcg
     2281 cgcgacctca aacgcttcct cgacgccgcc tcgctaccca cggtcgaacg ctacatgcgc
     2341 tggatcagcg tgctcgacca gacggcgaaa gacgatctct acaccgcgga tttccggcgc
     2401 gagatggaag gacacgaagc ctccgatttc gtcgcgccgt ggtttgccca agccaacgga
     2461 gcgggactcg tggatgcgtc gctgttggcc gacacgatga cctatttgcc gaacgacctg
     2521 ctcgttaaag ttgacatcgc ttcgatggcc gtctcgctcg aagcgcgctc tccgtttctc
     2581 gatcatcacg tgatagagtt tgccgcgagt ttgccggaga acctgaaact gcgcggcttg
     2641 acgacgaagt atctcttgaa acgcgtgctc gctaaactgc tccccgccga gaacctctcg
     2701 cgccgcaaga tgggcttcgg cgtgcccatc ggccactggc tgcgcggtga gatgcagcct
     2761 ttcctgcgcg agacgctgct ctcggaaaaa gctctggggc gcggcttctt ccgtcccgaa
     2821 gccgtcacgc gcatggtcga actccacacg cgcggcgaac gcgactacgc gcaccaactc
     2881 tggacgctgt tgatgcttga gttatggttt caacgcttta ttgattgaca cactcttatt
     2941 cggaggatcg ttattgtgaa aggtgttgta ctggcgggcg gcctcgggtc gcgtctgatg
     3001 ccgttgacgg ctgtgacgaa taagcacttg ttgccggttt ataatcagcc gatggtctac
     3061 tacccgattc agacgctggt gaacgcgggg atcagggaca tcatgatcgt gacgggcgga
     3121 aactcggctg gcgattttct caaacttttg aagaacggca aggcgttcgg cctcaagcat
     3181 ctcaactata cgtatcagga aggcgagggc ggcatcgcgg ctgcgctcgc gctctgcgaa
     3241 gacttcgccg acggcgaacc cgtctgcatc gtcctcggcg acaacatcat cgagggcaac
     3301 atctgtcagg ccgtgcgcgc ttaccggcat cagggcggcg gggcgaagat tctcttgaag
     3361 caagtctccg acccgcagcg tttcggcgtg cccgaactcg aaggctcgcg cgtcgtgcgt
     3421 atcgaagaga aaccgaaaga gccgaagtcg gactacgccg tgatcggcgt ctatatgtac
     3481 gacgcggaag tgttccagat catcaagacg ctgaagcctt ccgaacgcaa cgaacttgag
     3541 atcaccgatg tcaacaacgc ctacatcgcg cgcggcgaga tgacctggaa cgaactcgaa
     3601 ggctggtgga ctgacgcggg cacgttcgag agcctgttgc acgccggaac gctcgtcgcc
     3661 gagacgggcg cgaacaagat ggaggccgtc aatgcaaacc atgcctgagc gtgagccgca
     3721 ggggagcgcg ccgttcaagc gcgggcagat tcgcgatgtc gtcgtcagag acgtgcgcaa
     3781 gttcgtggac gagcgcggct ggctcgccga agtttttcgc cacgacgaga tggacgcgga
     3841 gtatttcccc acgatgtcct atatctcttc gaccgaaccg ggcgtgcagc gcggccccca
     3901 cgagcatgtg gatcaagccg atctcttcgt cttcatcggc cccgcgaatt tcaagctgcg
     3961 tctgtgggac aaccggcggg attcggaaac ttacaatcac gtgatgacgc tctacgtcgg
     4021 cgcggacaac ccgaaatcgg tgctcgtccc gaaaggcgtc gtccacgctt accgcaacgt
     4081 cggcagcacg gcaggcatcg tcatcaactg ccccaaccgc ctctacatgg gcgaaggcaa
     4141 gcgcgccgag atcgacgaga tcaggcacga agacgatccg gcgacgatct accggatgga
     4201 agattagcca gaaaacagaa gagagaattc aggagtcagg agaagacgac ttggtttcct
     4261 tctggctcct gaattctgta tcctgactcc tgctttatga aagttgttcg tatcatcgcg
     4321 cgtttgaacg tcggcggacc ggcaaagcat gtggcgtggc tgacggcggc gacgcggcgc
     4381 gcgggcgtgg agtcggaact cgtcgcgggg acggtgccgc cgggtgaaga cgacatgagt
     4441 tacttcgctg cggcgcaggg cgtcgagccg atcatcgtgg cggagatgag ccgcgaggtg
     4501 tcgccgaaag atgccctgac ggtgtggaaa ctctatcgcc tgttccgacg cttgcagccc
     4561 gacatcgtgc acacgcacac ggcgaaggcc gggacggtgg ggcgcgtggc gggcgtgctc
     4621 tatcgctggc tgacacccgc tgcgctcgcc gggcgaccgc gcccgtgccg cttcgtccac
     4681 acgtatcacg ggcacatctt tcatagctat tacgggcgac tcaagacgct cgtgttcctg
     4741 accgtcgaac gcgtgctcgc gcgcacggcg accgaccgca tcgtcgtcat cagccgccaa
     4801 cagtttcgcg agattcacga gcagttcggc gtgggacggg cgcggcagtt cagagtgatc
     4861 ccgctcggtc tggatgtcga ggcgtttgcc ggatggcgcg cgcggcgcga ggcggcgcgg
     4921 gctgcgttgg gggcgaagga agacgagttg ctcgtcggca tcgtcgggcg tctgacggag
     4981 atcaagaatc acaagttgtt tctcgaagtg gccgcgctct atttagaaag agacgcagcc
     5041 gcacaagcga cgggcgaagt cggcgagacg tttggcgcag cggcttcgcg tcgcgtgcgc
     5101 ttcgtggtcg tgggcgacgg gcatttgcgc gaagcgttgc aaacgcaggc cgacgcgctc
     5161 ggcatcgggg agcgcgtgac gttcacgggt ttgcgcgacg acccggaaaa tttttacgcc
     5221 gcgctcgaca tcgtcgcttt gacatctctg aacgagggca cgccgctcac gctcatcgaa
     5281 gcgatggcga acgcgcgtcc cgtcgtggcg accgccgtgg gcggcgtcgt cgatcttctg
     5341 ggcgaagcat cggacgatga accggcggcg cacgcaaccg cccccgccca cgcttacacg
     5401 atctgcgaac gcggggtgcg cgtgggcaca aacgacgcgg aggcgttttg cgcagccttg
     5461 cggcggctca tcacggatga agagttgcgg cgcacgctcg gcgagcgcgg gcgcgcgttt
     5521 gtcgcggagc attattccaa agagcgtctg gtggcggacg tgttgaatct ttacagtgaa
     5581 ctgttgccgt cacaaacgtc gccgacggcg gagcgcgttt cgagtcatgc gcggggcaaa
     5641 ggggcggcga cggcgaagac agcagcaaag caggggagcg tgccggggat tgaaacggcg
     5701 cgtttgaaag gagactaatt tttgcgagta ttaatcacgg gcggagcggg ctttatcggg
     5761 tcgcatctgt cggacgctta tctggagcgc ggcgacgaag tctattgcat tgacgatctc
     5821 tcgaccggca gttttgagaa catcgcgcat ctcaagacgc acccgcgttt tcattacacg
     5881 attgacagcg tgcataacgt gcccgtctgc gccgaactcg tagaccagtg cgacgtggtc
     5941 tttcatctgg cggcggcggt cggcgtcaaa ctgatcgtcg aaagccccgt cagaaccatc
     6001 gagacgaacg tgcgcgggac ggaagtgatt ctgagtctgg cgaacaagaa gaagaagaag
     6061 gtgctcgtcg cttcgacttc ggaagtttac ggcctctcgg atcaggtgcc tttccgtgaa
     6121 gacggaaacc tcgtgatggg cgcgacgacg aaagggcgtt ggagctacgc ctgctcgaaa
     6181 gcgattgacg aatttctcgc gctcgcctac tggcgcgaga agaagttgcc aacaatagtc
     6241 gtgcgcctct tcaacaccgt gggaccacga cagaccgggc agtacggaat ggtcatcccg
     6301 acgtttgtgc ggcaatctct ggcgggtcgc ccgatcacgg tctacggcaa cggcaaacag
     6361 acgcgctgct tcggctacgt gggcgacgtg gtgggcgcgc tgatgaagtt gatggacgcc
     6421 ccggaggcgg tcggtcaagt cttcaacatc ggttcgaacg aagaagtctc gattctggaa
     6481 gtggcggagc gggtgaagga gttgacggat tcgaaatccg agatcgtctt catgccctac
     6541 gacgaagcct acgaagaggg ctttgaggac atgccgcgcc gcgtgccgga catctcgaag
     6601 gtgagcgcgc tcatcggctt caagccgacg gagaaactcg acggcatcct ccaaaaagtg
     6661 atcgcgcacc acagcgggca gtaaagccgt gaatcgtaga tcgtgaatag agaagacggg
     6721 ttctttctat tcacgatcta cgattcacga ttcacgctct acttccccac ttgatttctc
     6781 acgctacatc gaatagcttt tcaagcgact tcaaaccaga atcaaccaca gttggatatg
     6841 tacgcgggta gcttaaatct cgttccgatt attgtgtcgt ttgcgctcgc gctcggtttg
     6901 accccgctcg tgcggcgttt ggcgcggcat tggggcatgg tggcgcagcc gaaggccgac
     6961 cgctggcaca acaagccgac cgccatgatg ggcggactcg ccatcttcct caccgtcgtc
     7021 atcgcttatc tcatcttcaa cgcgcagatg ccgcaccacc cttacgggtg ggtcgtgata
     7081 ctcggcagca cgtgtctctt cgtggtcggg ctggtggacg acatcctgca catcaagcct
     7141 tatcagaagt tgatcgggca ggtgatgggc gcgagtttga tcgtctatta cgggctgagt
     7201 ctgccgtgga gcggctcgcc ctcgctcaac atggcgatca cgatcttctg gctcatcggc
     7261 atcaccaacg cgctcaacct gctcgacaac atggacgggc tggccgccgg aatcgccgcc
     7321 atcgccgcgg gctttctctc cgcgagtttt ctctcgaacg gacgaccggc ggaagccctg
     7381 ttgctcggcg tcttctgcgc cgcgctcgtc ggctttctcg cctacaactc caaccccgcg
     7441 tcgatcttca tgggcgattg cggctcgatg ttcatcggct tctttctcgc cagcacggct
     7501 ttgatgagtg cggcgggcgc gcgttcgcgc tcgtttctcg tcgtgctcgc ggtgcccgtg
     7561 ctgatcctgc tcatcccgat cttcgacacg acttttgtga ccatcctgcg gaaactctcc
     7621 gggcgttcgg cctcgcaggg cgggcgcgac cacacttcgc accgcctcgt cgcgcttggg
     7681 atgacggagc ggcgcgcggt ctggatgctc tacgcgttcg cgggtctgtc gggcgtgctc
     7741 gcgctgctgg tgagccgtgt gaagtttgaa gtgagcgtcg ccgtcatcgt cgccttcacg
     7801 ctggtgctcg cgctcttagg cgtgtatctg gcgggggtca aagtttacga agaggaggag
     7861 gtcatgcggg cgcgcgaaaa accgctcgtc gccttcttca tagacctctc ctacaagcgg
     7921 cgcatcttcg aggtgatgct cgatgtcgtg ctcatcatcc tctcttacta cagcgcgtac
     7981 acgctgctct tcggggcgat gacggagacg ggcgcgtgga atctcttcct gcgcacggtg
     8041 cccgtgctgg tctgcgtgaa gatgttcgcg tttctggcgg cgggcgtcta tcgcggactg
     8101 tggcgttaca tcagcatcga cggcgtggtc gtgtttgcga aggcggtggt gctcggcgcg
     8161 gtcgggagcg tgctcgcgct gctggtcgcg ttccgtttcg agggcttgtc gcgcgccgtc
     8221 ttcgtgctcg acacgatgat gctctttatg atgctcgcgg gcagccgttt cgccttccgc
     8281 ctgtttcgcc acgcgctccc tgtggcggaa gcgagcggcg cgcggcgcgt gttgatctac
     8341 ggcgcgggcg acggcggcga actgctcctg cgcgaggtgc tcaacaatcg tgaactccaa
     8401 ctcacgcccg tcggcttcgt ggacgacgac ccgctcaaaa aaggaaaagt catacacggc
     8461 ctgcgcgtct tcggcggcaa cggcgcgctc aaaagcatct gcgagcagca acgcatcgcc
     8521 gaagtgttga tctcaagcac ccagatttcc gaagagaggc tcgccaccat cctccaggat
     8581 tgcgaagccg cacaggtcac gctcaaacgc atgcgcatcc agatcgatca actgaactga
     8641 acgaagcaga ggttagaggt tagaggtcag aggcgggtta aaaactgtct gagactaagc
     8701 tctgacctct aacctctgac ctctcgctta tgaaagacca gatcaagacc atcgaacttc
     8761 gcgccgtgtg g
//
LOCUS       DASWNS010000177         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_112028, whole genome shotgun sequence.
ACCESSION   DASWNS010000177 DASWNS010000000
VERSION     DASWNS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112028"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1307)
                     /locus_tag="VGB76_06095"
     CDS             complement(102..1307)
                     /locus_tag="VGB76_06095"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0098508.1"
                     /translation="MPTSTSQERKTKTSSGASAEAKGAATAANATSTLIEFPGVPPKP
                     QWRKELSERVREIQQRRALEAARESEPSAPQHSHISSGMIDDPTADVAAEVAAPPLGL
                     VPPPPEAPQPNPLVVAALKRIERARQSSQVPPMARASRGGAATAAAVAHVAEENYQPA
                     LEAPALVTDHAPATDLQTAPDALVEKIERPVEATRPSPLAIVPPTAAPPAPKTTEATA
                     TVAAAAQVTRARRHIPVVADEASLTQIEAQMHAAVISNHSLTPESATIDDHAPVAKRI
                     AGGIIDLFVVAFASTPFAAIIELTNGNWSDVRVAASMGGIVIVLMFIYLMAATALAGR
                     TWGMSVVSLRTVDAVTGMAPTTGQCIRRAFGYMLSLATLGVGLLYAFFDAEGRAAHDH
                     LSGTIVIAD"
     gene            complement(1743..2120)
                     /locus_tag="VGB76_06100"
     CDS             complement(1743..2120)
                     /locus_tag="VGB76_06100"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEY0098509.1"
                     /translation="MKLFTLSEANAMLPTVRRIAAAISRAHRQIISLQEAARAAAENA
                     TLGGGFMADGPQYVTDLLSLAERTSELEALGVQLKDYERGLLDFPSMREGRVVLLCWQ
                     LGEGDRIEWWHDLETGFAGRQPI"
     gene            complement(2220..2969)
                     /locus_tag="VGB76_06105"
     CDS             complement(2220..2969)
                     /locus_tag="VGB76_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY0098510.1"
                     /translation="MQHFDPTNNISWQLGYRGRVAVFIDGNNLFHAARFHNIDIDYNK
                     LLRVLLGDGRLLRAFFYTGVDVGAERQQGFLLWMRRNGFRVIHKELKTFYDGTRKANL
                     DVEIAVDMLSLAGRYDTAVLVSGDEDFVYAVNAVAYKGCRVEVAGFRSNSAPKLIDVA
                     DYFIDLGEIADKVRKEHSHGPRYYEHHPHEDSHTHSFLPSSEDVPLPEVRHEQLTQTT
                     TVTTAIAEIDITEDTEHFNSHSEFIRITDDH"
     gene            complement(3153..>3801)
                     /locus_tag="VGB76_06110"
     CDS             complement(3153..>3801)
                     /locus_tag="VGB76_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098511.1"
                     /translation="VLRLPLVEIRDEAGEVREQSREESRRESLRRFILGAGALLGVAP
                     QNLSLVGRTDEADGTRRALYQQHPFPYPLRAGFGVLEIAFTDDRRVLSLSSTAIPDTE
                     RLTRALALLRPQQLTADQAIERVRGRSFTLSGVNSPQTYTVAPDDPIVPRELVIYPVR
                     PANDQSALELHVAWEMAVGQGATPYLVYLDALRGEIIAATIQPAQAAATPTPPRQ"
BASE COUNT          716 a         1060 c         1273 g          752 t
ORIGIN      
        1 ggacaactga ccaccggcga ccggcgacgg gcaaccggca aacaacaaag ctctgcgttg
       61 cgtgaacaac gcagagcttt attgtacttg gtgcgagggt atcagtcggc gataacgatg
      121 gttcctgaga gatgatcgtg tgcggcgcgg ccttcggcgt cgaagaaagc gtagagcagc
      181 ccgacgccga gcgtggcgag cgagagcatg tagccgaagg cgcggcggat gcactggccg
      241 gtggttggag ccatgcccgt caccgcgtca accgtgcgca gcgagacgac cgacatgccc
      301 cacgtgcgcc ctgcgagcgc ggtggcggcc atcaggtaaa tgaacatcaa cacgatgacg
      361 atgccgccca tcgaagccgc gacgcgcacg tccgaccagt tgccgttggt cagttcgatg
      421 atggccgcaa acggcgtcga agcgaaagcg acgacgaaca gatcaatgat gccgcccgct
      481 atgcgcttcg cgacgggcgc gtgatcatct atcgtcgcgc tctccggcgt gagcgagtgg
      541 ttcgagatca ctgcggcgtg catctgggct tcgatctgag tgagcgaggc ttcgtcggcg
      601 acgacgggaa tgtgcctgcg cgcgcgcgtc acctgcgcgg cagcggcgac ggtcgcggtc
      661 gcttcggtgg tcttgggcgc gggcggcgcg gcagtaggcg gaacgatggc gagcggcgag
      721 gggcgtgtgg cctccacggg tcgttcgatc ttttcaacca gcgcgtccgg cgcggtttgc
      781 aggtcggttg cgggagcgtg gtcggttacg agtgcggggg cttcgagcgc gggctggtaa
      841 ttttcttccg cgacatgcgc gaccgccgcc gctgtcgctg cgccgcccct cgacgcgcga
      901 gccatcggcg gaacctgact cgactggcgt gcgcgctcga tgcgtttgag cgcggcgacg
      961 acgagcgggt tcggttgcgg agcttccggc ggaggcggga cgaggccgag cggcggcgcg
     1021 gccacttcgg cggccacgtc agcggtcggg tcgtcaatca tgccggaaga gatgtggctg
     1081 tgctgcggcg cggagggttc cgactcgcgt gcggcttcga gggcgcggcg ctgttgaatc
     1141 tcgcgcacgc gctcgctcaa ttccttgcgc cactgcggct tcgggggcac gccgggaaat
     1201 tctatgagcg tcgaagtggc gtttgccgcc gtcgctgcgc ctttggcttc tgcgctcgcg
     1261 ccggatgagg ttttagtttt acgttcctga ctggtcgaag tcggcataga ttcttcttcg
     1321 ggagacgggg aaggggcttc gactgctgct tcctgtgtcg ttaagcagag cgggcaattc
     1381 tccaacgaat cggagtaaga gccgccgcac acgacacact gcatagactg agagcctccc
     1441 ctgtggaaat cttcaaaatt aaatctttgg gatgacgggc gaagccggta aacccaagcc
     1501 ctttgcggag tcgcggcgga tgctatcaga gtttacttta agtcgtcaat cagaaaatac
     1561 acaaatcaat gtagttgggc ggcgggaagc cacaagatac gggatggaag gaagggtaaa
     1621 gggaaacgtt gaagggctaa acgggaggac gaagagaaga gagcgcctgc ggaaaagagg
     1681 gtctttaggt aagacgactg ctctatcctt tccccttcat cccttctcct ttttcctaag
     1741 ttttagatcg gctgtcgtcc ggcaaagccc gtttcgaggt cgtgccacca ctcgatgcgg
     1801 tcgccttcgc cgagttgcca gcagaggagg acgacgcgcc cttcgcgcat cgaaggaaaa
     1861 tccagcaagc cgcgctcgta gtctttgagt tgcacgccga gagcctccaa ctcgctcgtg
     1921 cgttcggcga ggctcaacaa gtccgtgacg tactgcggcc cgtcagccat gaagcccccg
     1981 ccgagcgtgg cgttctccgc cgccgcgcgc gccgcttcct gcaaggatat gatctgacga
     2041 tgagcgcgcg agatggctgc cgcgatacgc cgcaccgtgg gcaacatcgc gttggcttcc
     2101 gagagagtga aaagtttcat aaagcagtta cgagtgacga gtgacgagtg acgagccgga
     2161 atcaagacaa gcttttttgt ttttaacttg tcactcgtca ctcgtcaccc gtcactgttt
     2221 tagtgatcgt cggtgatgcg tatgaactcc gagtgggaat tgaaatgctc tgtgtcttcg
     2281 gtgatgtcga tctcggcgat ggccgtggtc acggttgtcg tctgcgtaag ttgttcgtgt
     2341 cgaacttcgg gcagcggcac gtcttcggac gagggcagga aagagtgtgt gtgcgaatcc
     2401 tcgtgcgggt gatgctcgta atagcgcggg ccgtgcgaat gttctttgcg cactttgtct
     2461 gcgatctcgc cgaggtctat gaagtagtcg gccacgtcta ttaacttcgg cgcgctgttg
     2521 gaacgaaagc cagcgacctc gacgcggcag cctttgtacg cgacggcgtt gacggcatag
     2581 acgaaatctt cgtcgccgga gacgagcacg gctgtatcgt agcgaccggc gagcgagagc
     2641 atatccacag cgatctccac gtccagattg gccttgcgcg tgccgtcgta gaaggttttc
     2701 agttctttgt gtatgacgcg gaaaccgttg cggcgcatcc agagcaggaa gccctgctgt
     2761 cgttccgcgc ccacatcgac gcccgtgtag aagaaggcgc gcagcagccg cccgtcgccg
     2821 aggagcacgc gcagaagttt gttgtaatcg atgtctatgt tgtgaaatcg tgctgcgtga
     2881 aaaagattgt ttccgtcaat gaaaaccgcg actcgccctc gatagcctaa ctgccaggaa
     2941 atgttattgg tcggatcaaa gtgctgcatg caaaaacacg ctccgttgtt aaattttttg
     3001 aaagctgaaa tttcatagtt ctaattgtga actctattga tctctaggat gccgccgcga
     3061 ggcgcatcag gtcaagcgaa aacagggcgc gggcgttcgt gcgagcgcgc cggacggctc
     3121 gccgtcacgg ttatcgtcct gccgccgtga ccttactgtc gtggcggtgt cggcgttgct
     3181 gccgcctgtg cgggctggat ggtggcggcg atgatctcgc cccgcaatgc gtcgagatag
     3241 acgagatagg gcgtcgcgcc ttgccccacg gccatctccc acgcgacgtg taattcgagc
     3301 gcgctctgat cgtttgcggg gcggacggga tagatgacga gttcgcgcgg gacgatgggg
     3361 tcgtcgggcg cgacggtgta agtctgtggg gaattgacgc cggagagagt gaaagagcga
     3421 ccgcgcacgc gctcgatggc ttgatcggcg gtcaactgct gcgggcgcag gagcgcgaga
     3481 gcgcgcgtca ggcgttccgt gtcgggaatg gctgtggaag agagcgagag cacgcgccgg
     3541 tcgtccgtga aagcgatctc caacacgccg aagcccgcgc gcagggggta gggaaaaggg
     3601 tgttgttgat aaagggcgcg acgcgtgccg tcggcttcgt ccgtgcgccc gacgagcgac
     3661 aaattctgtg gcgcgacgcc gagcagcgcg cccgcgccca gaatgaaacg gcgcaacgac
     3721 tcgcgccgcg actcctccct cgactgctct ctcacttccc ctgcttcgtc cctgatctcg
     3781 acgagcggca ggcgcaggac g
//
LOCUS       DASWNS010000178         5785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_8437, whole genome shotgun sequence.
ACCESSION   DASWNS010000178 DASWNS010000000
VERSION     DASWNS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8437"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(368..508)
                     /locus_tag="VGB76_06115"
     CDS             complement(368..508)
                     /locus_tag="VGB76_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098512.1"
                     /translation="MPCADLREVGDAAETFARDLREDAEACAKFWVADDTLPTSFGGN
                     SN"
     gene            583..2604
                     /locus_tag="VGB76_06120"
     CDS             583..2604
                     /locus_tag="VGB76_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098513.1"
                     /translation="MLTGSIKPARSRTNSWVYLLAAAAILLCSGQSSNVSAQGQWTTS
                     GTNINNTNSGNVGIGTATPTSALEVQAPDAPITLSQPGVAAKVTLQAALGTDMHLSAN
                     AKWTSNVWGRFNTLMPAWNFFLTPSGDYAGIRRAAPGNGAIAWTDLLRVSNTGNVGVG
                     TASPTARVHVVGANGTADSSGAAAPSAFLVTGGIGGAGNWYGGPGGTGGSIDLLGGNG
                     GTPQSGSGAAVGGPGGAINITGGSGGTSAFTASGIGGNVFINGGAMGASSPSGASGNV
                     ILANLRGFVGVGTASPAYKFDVAGSIRSSSGGFVFPDGTIQLTAAAGGGSSTTISAAN
                     VSAGQFGQNTGAGNFSFPASLLLNGSNSAIVDSSASNRFRICGPNGSCSLAGELIGGR
                     IRLSGTGSFTSAESMFDPGMGGIHAAGNAYIGGSVGIGTTTPNSPLTVSSNTNVTPML
                     VTGTNANVVGLVVNNSQAGSKAWGLQVTGSGNGYGAANGSLVIRQASDNINSLVVAPS
                     GNIGMGTSAPETKLHVVGDLKVSGNISARYQDVAEWVPSRQQLASGTVVILDSAHDNH
                     VVASSQVYDTRVAGVISPQPGLALGERGEGKVLVATTGRVLVKVDATRAPIRIGDLLV
                     TSNQGGVAMKSEPLNLGGVAIHRPGTIIGKALQSQEKGTGEILVLLSLQ"
     gene            2659..4875
                     /locus_tag="VGB76_06125"
     CDS             2659..4875
                     /locus_tag="VGB76_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098514.1"
                     /translation="MMNIFKRRRTGAQAVKLCVLAFLAMLCQAQSASAQWTTSGDHIT
                     NTNPGNVGVGTATPSAKLDIIGTVKAEAFSVGGASGEKTFSLLWSNDVANQKVQVYWP
                     ANTQVDGIFEITVTGHYWYSNSNGGIRKRIVINGRNIGVINMQQSEVPFRLGFTGSSD
                     TISNIMWDAANSRYYFIVSNLDNKQNGITIHVKSITPGATAANAEAMSMTPIYTTDTT
                     AYPQLYTSFMSGNIGVGTNTPGYRLDVQGGAVNASGGLCIAGDCKTSWSQVSSAASQW
                     TTAGSNIHFNTGNVGIGTSTPSQMFQVHSGSTWAGMRTTTAATGATVNDGANFGFDDA
                     VGAMIWNREASPIVFATSNAERLRVDALGNVGIGTTTPGMKLHVADMIYSQRAGEVGF
                     QMYNGGSVAEWAIRQRSAADHNLYFSKGVNGIKTDYMMIGTTGLVGIGTSAPGYRLDV
                     QNGAVNASGGLCIAGVCKTDWAQVGASQWTTAGANVHYGAGNVGIGNASPGSKLFVGS
                     GTPSVTTLPGINVALGGNTYVATSNGVVNTFIGSDISPYGIVGTLSNHPLGLRANNVL
                     AVTVQPSGNVGIGTAAPETKLHVMGDLKVSGNISAKYQDVAEWVPSLETLSAGMVVIL
                     DPEHVNHVQSSNTSYDTSVAGVVSAQPGLSLGEPGEGKALIATTGRVRVKVDASRAPI
                     RIGDLLVTSDLPGVAMKSEAVAVGGRKMHSPGTIIGKALEPLAKGTGEILVLLSLQ"
     gene            4978..5730
                     /locus_tag="VGB76_06130"
     CDS             4978..5730
                     /locus_tag="VGB76_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098515.1"
                     /translation="MNRRILASLGIVLCAVIASAGALAMKKRTTSKPRPTTAAPATST
                     NTSRQGETLVISPAGERQQQPQPQANVPEPQANVPEHVVYWHIFHHHNFLNRKADEAQ
                     RMGKAEEATRLRGFYKREAKLDEGQDAALGQIALEVENEVEVLDAQAKRVIDAAREKT
                     PGGALKKDEAIPPPPRELAALQERRNAAILKARNRLKSMLGDAAFDEFQGFVREKVAP
                     QIKQKPFDVPRPDAPAGGRRQPRNNPYEGRTR"
BASE COUNT         1196 a         1893 c         1749 g          947 t
ORIGIN      
        1 tcccgcttga cattcaccgg caatacattt atcttgcaca ctctttcgcg ggacctcgct
       61 gccgaacctc ctctcgtcag ccttccataa agcagctaac caggatcaga tgtagtgcaa
      121 ttccctctta cggtaactta ctgtcaaaag gcagcagtgt atctgctttg tgtgacacgt
      181 cctctgtatc acagcgtaat aatctccggc tgggaggtta cgctgaacat ataatcaaag
      241 ccccacaatc acttgccggt gaccgtgggg ctttttaaca acgcaactac cgccgccgaa
      301 gcgtacagaa gatgcaccgt ttgaagagcc cgcatcttag cgcattccgc gaccttcaat
      361 caaccattta attcgagttg ccaccgaacg aagttggcag cgtgtcgtct gcgacccaga
      421 acttggcaca ggcttcggca tcctcccgca agtcgcgcgc aaaagtctcc gcagcatcgc
      481 cgacctctct caagtctgcg catggcacgc gttcgtgatt ttcatcagcc ggagtgtgcc
      541 tgccgcccgc tctcgctcat aagtccacac ggagtaaaga agatgttaac cggatcaatc
      601 aaaccggcgc gctcgcgcac aaactcttgg gtttacctgc tggcggctgc ggccatattg
      661 ctttgttcag gccaatcgtc gaacgtgtcc gcacaaggcc aatggacgac gagcggcacg
      721 aacattaaca acaccaattc cggcaacgtc ggcatcggca cggcgacccc cacgagcgcg
      781 ctcgaagtgc aggcacccga cgcccccatc accctctcgc agccgggcgt cgcggctaag
      841 gtcacgcttc aggcagccct cggaacggac atgcacctga gcgccaatgc caagtggacc
      901 agcaacgtgt ggggacgctt caacacactt atgcccgcct ggaacttctt cctcacgccg
      961 agcggagact acgcgggtat ccgccgcgcc gcgccgggca acggagcaat cgcatggaca
     1021 gacctcctca gggtcagcaa cacgggcaac gtcggcgtcg gcacggccag cccgaccgcc
     1081 agagtgcatg tcgtcggagc taacggcacg gcagactctt cgggcgcggc ggctccttcg
     1141 gccttccttg tcacgggagg aatcgggggc gcgggcaact ggtatggcgg acccggcgga
     1201 acgggcggca gcattgacct gctgggcggc aacggcggga ctccgcaatc gggcagcggc
     1261 gcagccgtgg gtggaccggg cggcgcgatc aacattacag gcggcagcgg cggcacatcc
     1321 gctttcaccg cttccggcat cggcgggaac gtattcatca acggcggcgc gatgggcgca
     1381 tcgtctcctt cgggcgcgtc gggaaacgtc atcctcgcca acctgcgtgg cttcgtcggc
     1441 gtcggcaccg ccagtcccgc ttacaaattc gatgtcgccg ggtctatccg ctcaagttcg
     1501 gggggctttg ttttcccgga cggcaccatc caactcacgg cagccgcggg cggcggctct
     1561 tccaccacca tctccgccgc aaacgtctcg gcaggacagt tcggacagaa cacgggagcg
     1621 ggcaatttta gtttccccgc ctcactgctg ttgaacggta gcaactctgc catcgtcgat
     1681 tcgagcgcga gcaatcgctt tcgcatctgt ggtccaaacg gcagttgctc cctggcgggc
     1741 gaacttatcg gcggcaggat tcggctctcc ggcacgggaa gttttaccag cgcggagtcg
     1801 atgttcgatc ccggcatggg cggtattcac gcggcgggta acgcctacat cggcggcagc
     1861 gtaggcatcg gcacgacgac tcccaacagc ccgctcaccg tatcgagtaa cacgaacgtc
     1921 acgcccatgc tcgtcaccgg cacgaacgcc aacgtcgtcg gcttagtcgt taacaactcg
     1981 caggccgggt cgaaggcgtg gggactacag gtgacgggat cgggcaacgg ctacggggcg
     2041 gccaacggct cgctcgtcat ccgtcaggca tccgacaaca tcaactcgct ggtcgtcgcc
     2101 ccttcgggca acatcgggat gggcacgtcc gcaccggaga cgaaactgca tgtcgtgggc
     2161 gacttgaagg tgtcgggcaa catctcggcc aggtatcagg acgtggccga gtgggttccg
     2221 tcccggcagc aactcgcttc gggcacggtc gtcattctcg actccgcaca cgacaaccac
     2281 gtcgtcgcct cttcgcaggt gtatgacacg cgggtggcag gcgtcatctc gcctcaaccc
     2341 ggcctcgcgc tcggcgagcg cggcgaaggg aaagtactcg tcgccaccac ggggcgcgta
     2401 ctcgtcaaag ttgacgcaac gcgcgctccc atacgcatcg gcgacctgct cgtgacgagc
     2461 aatcagggcg gcgtcgcgat gaaatcggag ccgctcaacc tgggcggcgt cgccattcac
     2521 cgtcccggta cgatcatcgg caaggctttg caatcacagg aaaagggcac gggcgaaatt
     2581 ctcgtcctcc tcagcctgca atagtttccc ccaatcaacg aactcagcaa ctactaaaga
     2641 cgcgaggaaa gagacgcaat gatgaacatt ttcaaacgac gcaggacggg tgctcaggcc
     2701 gtcaagcttt gtgtgttggc cttcctggca atgctgtgtc aggcgcagag cgcatcggcg
     2761 cagtggacga ccagcggcga ccacatcacc aacaccaacc ccggcaacgt cggcgtcggc
     2821 accgccaccc catcggcgaa gctcgacatt atcggcaccg ttaaggcgga ggctttcagc
     2881 gtcggcgggg cgtccggcga aaaaactttc tccctgctct ggagcaacga cgtggcgaac
     2941 cagaaggtgc aggtctactg gccggccaac acacaggtag acggtatttt cgagatcacc
     3001 gtcacgggtc actactggta ttccaactcc aacggcggca tccgcaagcg catcgtcatc
     3061 aatggccgca acatcggggt catcaacatg cagcagagcg aggtgccgtt ccgcctcggc
     3121 ttcaccggca gcagcgacac gatctcgaac atcatgtggg atgccgccaa cagtcgttac
     3181 tacttcatcg tgtcgaacct cgacaacaag cagaacggca tcaccatcca cgtcaagtcc
     3241 atcacgccgg gcgcaaccgc tgccaacgcc gaggccatga gcatgacgcc gatctatacg
     3301 accgacacga ccgcttatcc acagctctac acctctttca tgagcggcaa catcggcgtc
     3361 ggcaccaaca cgcccggcta ccgtctggac gtgcagggcg gcgcggtcaa cgcttccggc
     3421 ggcctctgca tcgccggaga ctgtaagacc tcctggtcgc aggtcagcag cgcggcgagc
     3481 cagtggacca ccgccgggtc gaacattcat ttcaacacgg gcaacgtcgg catcggcacc
     3541 tcgacgcctt cgcagatgtt tcaggtgcac agcggcagca catgggcggg catgaggacg
     3601 acgacggcag ccaccggcgc gactgtcaac gacggcgcga acttcggttt cgacgatgcg
     3661 gtcggagcga tgatctggaa tagagaggca agccccatcg tcttcgccac gagcaacgcg
     3721 gagaggcttc gcgtggacgc tctcggtaac gtcggcatcg ggacaacaac gcccggaatg
     3781 aagttgcacg tcgccgacat gatctactcg cagcgcgcgg gcgaggtcgg cttccagatg
     3841 tacaacggcg gctccgtcgc cgagtgggcg atacgccagc ggagtgccgc cgaccacaac
     3901 ctctatttca gcaagggcgt caacggcatc aagaccgact acatgatgat cggcacgacc
     3961 ggccttgtcg gcatcggcac gagcgcgccc ggctaccgtc tggacgtgca gaacggtgcg
     4021 gtcaacgcct ccggcggcct ctgcatcgcg ggcgtctgca agaccgactg ggcgcaggtg
     4081 ggcgcaagcc agtggacgac tgcgggagct aatgtccact acggagcggg caacgtcggt
     4141 atcggcaacg ccagccccgg ctcgaagctc ttcgtcggtt cgggcacgcc ttcggtgacg
     4201 acgctgccgg gcatcaacgt cgcgctcggc ggcaacacat acgtcgccac gagtaacggt
     4261 gtggtcaaca ccttcatcgg ctccgacatc tcgccctacg gcatcgtcgg gacgctctcc
     4321 aaccacccgc tcggcctgcg cgctaacaac gtgctcgcag tgaccgttca accgagcggc
     4381 aacgtcggca tcggcaccgc cgcgccggag acgaaactgc atgtcatggg cgacctgaag
     4441 gtgtcgggca acatctcggc caagtatcag gacgtggccg agtgggttcc ttcgctcgaa
     4501 acgctgtcgg cgggcatggt cgtcattctc gacccggagc acgtcaacca cgtgcaaagc
     4561 tccaacactt catacgacac gagcgtcgcg ggcgtcgtca gcgcacagcc cggtctgtcg
     4621 ctcggcgaac ccggcgaagg caaggcgctc atcgccacca ccgggcgcgt gcgcgtcaag
     4681 gttgacgcct cgcgcgctcc catacgcatc ggcgacctgc tcgtgacgag cgatctcccc
     4741 ggcgtggcga tgaagtccga agcggtcgcg gtcggcggca ggaagatgca ttcgccgggc
     4801 acgatcatcg gcaaggctct ggagccgctg gcgaagggca cgggcgaaat cctcgtcctc
     4861 ctcagcctcc agtaagagag gttcgacgct agggagcggc ggggcttgcc cggaggcgac
     4921 cccgccgacg cagaactttc gcggctcaac agcctcaaaa gtattcagga gataatgatg
     4981 aacagaagaa ttttagcatc actcggcatc gtgctgtgcg ccgttatcgc gtcggcgggc
     5041 gcgctggcga tgaagaagag aacgacatcg aagccgcgcc ccacgacggc ggctccggcc
     5101 acttccacga atacgtcgcg gcagggcgaa acgctcgtca tctcgcccgc aggcgagcga
     5161 cagcagcagc cgcagccaca ggcgaacgtg ccggaaccgc aggcgaatgt gccggagcac
     5221 gtcgtctact ggcacatctt ccaccaccac aacttcctca accggaaggc cgacgaagcg
     5281 cagcggatgg gaaaggccga ggaggccacg cgcttgcgcg gcttctacaa gcgcgaggcg
     5341 aagctcgacg aagggcagga cgccgccctc ggccagatcg ccttagaggt agagaacgaa
     5401 gttgaggtgt tggacgcgca ggccaagcgc gtgattgacg cggcgcgcga gaagacaccc
     5461 ggcggcgcgt tgaagaagga cgaggcgata ccgcccccgc cgcgcgaact ggccgcgcta
     5521 caggagcggc gcaacgctgc gatcctcaag gctcgcaacc ggctcaagtc aatgctgggc
     5581 gatgcggcct tcgacgagtt tcaaggcttc gtgcgcgaga aggtggcgcc gcagatcaag
     5641 cagaaaccgt tcgacgttcc gcgccctgac gcgcccgctg gcggtcgccg ccagccgcga
     5701 aataatccct acgagggtcg cacccgctga ggcgcacgac gcgccacggc atcggcaaat
     5761 agacagtccc ctaacatcat agaga
//
LOCUS       DASWNS010000179         4357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_345029, whole genome shotgun sequence.
ACCESSION   DASWNS010000179 DASWNS010000000
VERSION     DASWNS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345029"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1950
                     /gene="dnaK"
                     /locus_tag="VGB76_06135"
     CDS             103..1950
                     /gene="dnaK"
                     /locus_tag="VGB76_06135"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY0098516.1"
                     /translation="MSKVIGIDLGTTNCCVSVMEGGTVQIIPNKEGGRTTPSVVGFTD
                     KGERLVGQIAKRQAVTNAANTLYAVKRLIGRKFDSPEVQRALETTPFEITDSQNGDAR
                     IRVQGRVYSPQEISGIVLQRLKAAAEDFLGEAITEAVVTVPAYFDDTQRQATKDAGKI
                     AGLRIERIINEPTAAALAYGLGRSASERIVVYDLGGGTFDVSILEMNDGVFEVLSTSG
                     NTFLGGEDFDERIINWMVDQFREETSINLREDRLALQRLKEAAERAKCELSSLTETNI
                     NLPFIAADASGPKHFSKTLTRETFEGLVADLVEQTIEPCQKALWDAKLQPDNIDKVLL
                     VGGQTRSPIIARTVREIFGREASSEINPDEVVGMGAAIQGGVLTGEVKDIVLLDVLPL
                     SLGLETRGGLFTKLIERNSTIPLKSSLTFTTVADNQSSVEIHVLQGEREIAGGNRSLG
                     KFELVGIPPGPRGSAQIEVSFEVDASGLLSVSAQEKATGRQQQMRITPTSGLNPEEID
                     NLIAEASTSASKDQRAKEVIHMRNRLDSLSRSAQRTFKELSSALSSEERQQAVSVFSE
                     CAEAAESTDEEEIKRALANVERIAALLTNAMLSGATSSPNTPASNKEVG"
     gene            2057..3187
                     /gene="dnaJ"
                     /locus_tag="VGB76_06140"
     CDS             2057..3187
                     /gene="dnaJ"
                     /locus_tag="VGB76_06140"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY0098517.1"
                     /translation="MSKKDYYEVLGVGRNATEQELKSAYKRLAIRYHPDKNPGDAEAE
                     EMFKEVAEAYSVLSNTEQRARYDRFGHAGVSSSAAGAGANWGAQGFGGIEDILGDLFG
                     FGDVFGGRAGAGGGRRSTGQRGADLRYDLEITLEEAAEGMTAQLRIPRLETCDTCTGT
                     GAKSGTQPETCSTCSGAGQVRYQQGFFSVARTCGACRGTGRIIKNPCETCKGAGRVER
                     ERQMEVKVPAGVETGSRLRITGEGEGGASGGGAGDLYVVIHVKEHEQFERQGSNLYAA
                     VPVTFAQAALGSEIMVKTLAGEQPLKIPAGTQTGTVFRLKGHGMPVLGGRGHGDLFVS
                     VALRTPTTLTREQRRLLEELSKIEDRDLEDKGLIDRVRDIFG"
     gene            3563..3913
                     /locus_tag="VGB76_06145"
     CDS             3563..3913
                     /locus_tag="VGB76_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098518.1"
                     /translation="MKKFNRAIFGILAFALMLGANVAQAASLDDNDTEGRLVNVRVVE
                     VSDARISVVTPDGVEHVISVCQINTQVRRGKQYVHCKDLRVDDVVTIVLDEEKPIKFA
                     KNIEVREMVAENKP"
     gene            complement(4116..>4357)
                     /locus_tag="VGB76_06150"
     CDS             complement(4116..>4357)
                     /locus_tag="VGB76_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098519.1"
                     /translation="NGALRDASDAAGRRLGIPVYFPSRHLSTDNAAMIAAAGTVKLQT
                     GERADLSLNADVSLRLGNIDAAEDAARRGRIRYRQ"
BASE COUNT          961 a         1258 c         1329 g          809 t
ORIGIN      
        1 tattcgtcat tcattagtca aagtatcgac ctcgaccaat cccgaagcgg attagcaaca
       61 gcttctcata cttaacttta taaatttaag gactcgcatt ttatgagcaa agtcatcgga
      121 atagatttag gaacaaccaa ctgctgcgtt tcggtcatgg aaggcggcac ggtgcagatt
      181 attccgaaca aagaaggcgg gcgcacgacg ccttccgtgg tcgggtttac ggataagggc
      241 gaacggctcg tcgggcagat cgccaagcga caggcggtga ccaatgccgc caacaccctc
      301 tacgccgtca aacgactcat cggtcgtaag ttcgattcgc cggaagtgca gcgcgcactc
      361 gaaaccacgc cgttcgagat caccgactcg caaaacggcg acgcccgtat acgtgtgcag
      421 ggacgcgtct atagcccgca ggagatttcc gggatcgtcc tgcaacgctt gaaagccgcc
      481 gccgaagatt ttctcggcga agcgatcaca gaggccgtcg tcaccgtgcc cgcttatttc
      541 gacgacacgc agcggcaggc gaccaaagac gcgggaaaga tcgccggact ccgcatcgag
      601 cgcatcatca acgagccgac cgcagccgca ctcgcttacg ggcttggtcg ctccgcctcc
      661 gagcgcatcg tcgtctacga tctgggcggc ggtacgttcg acgtttcgat ccttgagatg
      721 aacgacggcg tcttcgaggt gctgtcaact tcgggcaaca ctttcctcgg cggcgaagat
      781 ttcgatgagc gcatcatcaa ctggatggtc gatcagtttc gcgaagagac gagcatcaac
      841 ctgcgcgaag accgcctcgc cctgcaacgt ctgaaagagg ccgccgaacg cgccaagtgt
      901 gaactctctt cactgacgga gacgaacatc aatctcccct tcatcgccgc agatgcctcc
      961 ggccccaagc atttcagcaa gactctcacg cgcgagactt tcgagggact cgtcgcagac
     1021 ctcgtcgagc agaccatcga gccatgccag aaggcgctct gggatgccaa gctgcaaccc
     1081 gacaacatag acaaggtgct cctcgtcggc ggtcaaaccc gttcgccgat catcgcgcgc
     1141 acggtgcgag aaattttcgg gcgcgaggct tcttccgaga tcaacccgga cgaagtggtc
     1201 gggatgggcg cggcgattca gggcggcgtg ctgacgggcg aagtgaaaga catcgtgctg
     1261 ctcgacgtgt tgccgctctc gctcggcctg gagacgcgcg gcggcctctt caccaaatta
     1321 atcgaacgca attccacgat tcctctcaag agcagtttga ccttcacgac cgtcgctgac
     1381 aatcaatcga gcgtggagat tcacgtcctg caaggcgagc gtgagatcgc cgggggcaat
     1441 cgctcgctcg gcaagtttga attggtcggc atcccgccgg gtccgcgcgg gtcggcgcag
     1501 atcgaagtga gctttgaagt tgacgccagc ggactgttga gcgtctccgc gcaggagaag
     1561 gcgaccgggc gtcagcagca gatgcgcatc acgcccactt ccggcctgaa cccggaggag
     1621 attgataatt tgatcgccga agccagcacc tccgcgagca aagaccagcg cgccaaagag
     1681 gtcatacaca tgcgcaaccg gctcgacagt ctctcgcgca gcgcgcaacg cactttcaag
     1741 gagttgtcga gcgcgctctc ctccgaggag agacaacagg ccgtgagcgt cttcagcgag
     1801 tgcgcggaag ctgcggaatc cacggacgaa gaagagatca aacgcgcgct cgccaacgtc
     1861 gaacgcatcg cggccttgct gaccaacgcc atgctgagcg gcgcaacctc gtctcccaat
     1921 acgcccgcat cgaacaaaga ggtcggctga aatttgaaga accctgacaa gtgacgagcg
     1981 acgagtgaca agttataata ggttttagtt ttaacttgtc acctgtcact cgtcacttgt
     2041 tactgcttta ggagctatga gcaaaaagga ttattacgaa gtattagggg tcgggcgcaa
     2101 tgcgaccgag caggagttga agagcgcgta caagcgtctc gctattcgtt atcatccgga
     2161 taaaaacccg ggcgacgcgg aagcggaaga gatgttcaag gaagtggccg aggcttacag
     2221 cgtgctctcg aacacggagc agcgtgcgcg ttatgatcgc ttcgggcacg cgggcgtctc
     2281 ttcgagcgcg gcgggcgcgg gcgccaactg gggcgcgcag ggcttcggcg gcatcgaaga
     2341 tattctcggc gacctcttcg gcttcggaga cgtgttcggc gggcgagcgg gcgcgggcgg
     2401 cggtcgtcgt tccaccgggc agcgcggcgc ggatttgcgc tacgacctgg agatcactct
     2461 ggaagaagcg gcggaaggga tgacggcgca actgcgcatc ccccggcttg agacgtgcga
     2521 cacctgcacg ggcacgggcg cgaaaagcgg cacgcagccg gagacttgtt cgacgtgcag
     2581 cggcgcgggg caggttcgtt atcaacaagg cttcttctcc gtcgcgcgca cttgcggcgc
     2641 gtgtcgcggc acggggcgca tcatcaagaa tccctgcgag acgtgcaagg gcgcggggcg
     2701 cgtcgaacgt gaacgacaga tggaagtgaa ggttccggcg ggcgtcgaga caggatcgcg
     2761 cctgcgcatc acgggcgaag gcgagggcgg cgcgagcggg ggcggcgcgg gcgatctcta
     2821 cgtcgtgatc cacgttaaag agcacgagca gtttgaaaga cagggcagca atctttacgc
     2881 ggcggtgccc gtcacgtttg cgcaggccgc gctcggttcg gagatcatgg tgaagactct
     2941 ggcgggcgag cagcctttga agattccggc gggcacgcag acgggcacgg tctttcgtct
     3001 caaaggccac ggcatgcccg tgctcggcgg gcgcggacat ggcgacctct tcgtctccgt
     3061 cgccctgcgc acgcccacta cgctgacgcg tgaacagcgt cgtcttctcg aagaactctc
     3121 caagatcgaa gaccgcgacc ttgaagacaa gggactcatc gaccgcgtcc gcgacatctt
     3181 cggctaaacc tcagaccaaa cgattcacca cacggacgcg gagcaaagcc gcagaggaaa
     3241 cgcagagagt taaaaaactc ttctccgcgt caactctgcg gctttgttct cgtgtcgctg
     3301 ccgtgagtct aatttccggc cactcttacc ccgatgtcct gcaacaatac aaatctcgcc
     3361 gttgtcgcgt aataatacgg acgccattta gtcgcctagg ctcacaggaa atttccaatc
     3421 gagcacactt tcccgcgtat ttcgcacaaa ctttcgtatc gacggattcc gccgcagaga
     3481 ggcacaacgc ttgctctgta gtgcggcagc gaacttgtcc gcgtctctcc caaaaacaga
     3541 tgtggcgacg ggagttaaga agatgaaaaa attcaatcgc gcgattttcg gtattttggc
     3601 tttcgcattg atgctcggag caaacgtcgc tcaggcggcg agcctggatg acaacgacac
     3661 ggaggggcga ctggtcaatg tccgcgtcgt ggaagtgagc gacgcacgca tttcggtggt
     3721 aacgcccgac ggcgtcgagc atgtcatttc cgtctgccag atcaacacgc aggtgagacg
     3781 cggcaagcag tatgtgcatt gcaaagattt gcgcgtggac gacgtggtga cgattgtgct
     3841 cgacgaagag aagccgatca agtttgcgaa gaacatcgaa gtcagggaga tggtggcgga
     3901 aaacaagccc taacgatcaa tttctaaaga gttcgccggt gagccggtgg cgttctacgg
     3961 aacgccccct cgcacactca ccggcaacaa agagccgctt ggccttgacg ggtcaagcgg
     4021 ctttcagttt aaagatgaag taggaacgcg gaacgcggaa cgaaagcaac atgtctttca
     4081 gttcatcgtt ccgcgttcct acttcatcgt tatttctact gtctgtagcg gatgcgaccg
     4141 cgccgtgcag catcttcggc ggcgtctatg tttcccagac ggagagagac atcggcgttg
     4201 agggagaggt cggcgcgctc gcccgtctgg agtttgaccg tcccggcggc ggcgatcatc
     4261 gcggcgttgt ccgtcgagag atggcgggaa gggaaataga ccggaatgcc gagtcgcctg
     4321 cccgccgcgt cagacgcgtc tctgagcgcg ccgttac
//
LOCUS       DASWNS010000180        12466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_190089, whole genome shotgun sequence.
ACCESSION   DASWNS010000180 DASWNS010000000
VERSION     DASWNS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12466
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190089"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..794)
                     /locus_tag="VGB76_06155"
     CDS             complement(join(13..455,455..794))
                     /locus_tag="VGB76_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611655.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY0098520.1"
                     /translation="MARGEELTDEQWAILAPLIPEPQRREDKRGRPWRDTREVLNGVL
                     WILRSGARWQDLPDRFPPYQTCHRRFQQWSRDGTLRRVLEALAEDLRERGEVDFSECF
                     IDGTFVTAKKGGRGVGKTKRGKGTKLMVVADNASLPLAVHAASASPHEVTLVGETLLQ
                     SFTAGRPERLIGDKAYDSDPLDAELAGRGIELIAPHRKNRKKPRTQDGRKLRRYRRWK
                     VERLNAWLQNFRRVATRFDYHVENFLGFVHLGCIKILLRCYL"
     gene            complement(998..1710)
                     /locus_tag="VGB76_06160"
                     /pseudo
     CDS             complement(998..1710)
                     /locus_tag="VGB76_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            1952..4489
                     /locus_tag="VGB76_06165"
     CDS             1952..4489
                     /locus_tag="VGB76_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098521.1"
                     /translation="MLSLLARSRSLLRGRGARGLRAFDSLRFSVLDLKLGGRMLVKHP
                     ALTVIATIAVGFAVAVGTVGFEIARQALWPTIPLPNGDAIVALQNWNAAESVRIDASR
                     RDYELWRDGLSTITDLSAVEVEERSIAVGAGPGQPETVANVTASTFALTRVPALMGRT
                     LLGADERGGEEEVVVVGYDFWKNKLDGAADAVGRVIRISGTPVTIVGVMPRGYGFPHR
                     NDVWRPLHLEQIPEASPSLGYVFGRLAPGRTHEEASAELAVIGARAATTFPETNKNVR
                     LQVLSLPDAVSDLPEAAALVLASINVFLVLLAALLCGNVGMLLFARAVSREREFLVRA
                     VLGASRGRLIMQLFAEALLLCAVGAVVGLLVARFVLARAWTEIEVQMGPLPFWIDTSL
                     STTTILYAVGLTLFAAAIAGVVPALKVTSGGASGRLQAASSGGGGLRFGGVWTVVIVA
                     QIAFTTLLPWPLLGLGGAIGGDTPAGFPAEAFLTATLEIDRFDGVAASGDTAPAVRAA
                     RLEARYRALADRLRQDPGVLDVTYADQMPQLITSNFRAIEMDPGPAAAPGENCVGCDY
                     AKVVSVDPRFFDVVGAPVISGRALTTADAEHRTRAVLVNEYFVDQVLGGRNPIGRRFR
                     FSSTGEPTAESWYEIVGVVPDLSVSDTGTPYGRRIYQTTLPRETGPLRVAIHVRGDPQ
                     RFAVRLRELAQATDPALRVIDPKPLSLLEDPGSAGLSYGFELMVSLTLVTLMLSLAGV
                     YAVTAFSVARRTREIGVRVALGAQPLHILTTVFKRPLIQVAMGIGVGAILGFGLANDD
                     LSKMHLDVLGKTAVFALSTILCCALACIVPARRALRIQPTEALRDDG"
     gene            4595..5308
                     /locus_tag="VGB76_06170"
                     /pseudo
     CDS             4595..5308
                     /locus_tag="VGB76_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            5657..6487
                     /locus_tag="VGB76_06175"
     CDS             5657..6487
                     /locus_tag="VGB76_06175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0098522.1"
                     /translation="MRTVLLVSNALLLLLLIVAARRMLNYRSRLRRLELNYRSQLKAI
                     EGTFDSVIPLVRLDEFHEVFAQTGSGFNPETEVSFIGCWVRGGTSCFESWVLSVLAKR
                     ATSLFEFGTCSGKTTYLWAKNSPAEARIITLTLGKDQHADYLAAGEDNLLATRAALDE
                     STFDRFIYSGTPVEHKITQLFGDSKRFDETPYLKQCDLIFIDGSHAYSYVKNDTEKAL
                     RMLKEGGLLLWHDYRDRNVETRGVRRYLDELSETLPLIRLSDTTLVAYRNGTNKVAAD
                     "
     gene            complement(6512..7696)
                     /locus_tag="VGB76_06180"
     CDS             complement(6512..7696)
                     /locus_tag="VGB76_06180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0098523.1"
                     /translation="MSSKPELLYLSPVVPALAGNGLAMRAGMVLEALAEHYSISLLVV
                     SLYPPFAARVPAAFERLCLRTVVTTPSQFARTRVSFLPQFLSRPFGRRPYHGSRFDVV
                     HVFRLSMMPYARPFLNVSSGTPEGHLDLDDIESETHRRIAALCRLNKDGPRAAYYEME
                     ARRYEALEQEVLREFERVYVCSEGDKARLHADHGSELCVLPNAVRLPDVGRRDANEGV
                     FTFLFVGTLGYYPNEDAVRYFGSEILPHIRRLTRSDFVVNIVGSGAGPRLRQIAVDYG
                     MRMIGEVPDVAPWYQNSTAVVVPVRAGGGTRIKVLEAFSHRRAVISTSIGMEGIDAVD
                     DVHVLTANTPEVFAERCVRLMADRVLRERLAENAFSLLRHHYSAEAIKRTIASLLVSP
                     VY"
     gene            complement(7772..8779)
                     /locus_tag="VGB76_06185"
     CDS             complement(7772..8779)
                     /locus_tag="VGB76_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HEY0098524.1"
                     /translation="MSNVEEAGYSSMGDTSTIKILVYDFKWGASLAPTSACAPGYEII
                     SDPKWLRDADVVVFHIPTAPELRGLRKRPGQKWVAWSFESDRFYPRLCDPKYMRQFDL
                     TMTYRWDSDVPVAYFGPDTIDELLRAPALKTASAPAAAFVSNGIDLSGRFKYMQELMQ
                     CLPVDSYGLTLQNKKLPEDKRRETKLETIAAYKFTLAFENSISQDYVTEKFFDPLIVG
                     SVPVYLGAPNIDDFAPGEHCYINVAQFKGPRELAEYLIFAAGNQSEYDSYLAWKTEPL
                     RQIFLDKVEVMAKDSFLRLSEKVRQMSAADRAGVGFADRLTSTVSAVVKTVQHRLGLR
                     E"
     gene            complement(8776..9522)
                     /locus_tag="VGB76_06190"
     CDS             complement(8776..9522)
                     /locus_tag="VGB76_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEY0098525.1"
                     /translation="MKKEDCTIALPPIWVANLARSPERRRFMQEQFASLDLSYRIINA
                     TDGKQLSRGELQKYSKPHALKIKRRELSTGEIGCALTHAKIYQQMLDENLDEVLILED
                     DIVLTQDLLHVLLQRRKFPPEWEAVNFANTWAKAIPLEGPVCKDYRVCRFRGIANRTS
                     AYLLNRQGAKKLIEHVYPIRLPADDFVGLTYITGLHLYGVTPSVVALADFKSDIWSDG
                     GGPNEPTFNDRKMWARMRKKGTMMWSRLFG"
     gene            complement(9534..10334)
                     /locus_tag="VGB76_06195"
     CDS             complement(9534..10334)
                     /locus_tag="VGB76_06195"
                     /inference="COORDINATES: protein motif:HMM:NF015417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098526.1"
                     /translation="MPILELLSNLITPGKNAVKGRDRILILTPVKNASVFIQGYCRRL
                     RQLTYPHKSISVGLLESDSNDSTYRALQAHIPVLRRQFRRVGLWKRDFGYHVPSGVHR
                     GEEAIQVERRTVLARSRNHLLMHALDDEDWVVWLDVDVIEYPPDIIERLLATGKEIVH
                     PHCVLEFGGATFDRNAWRDKGRYHMDDLRREGELVELDAVGGTMLLVRADLHRDGLIF
                     PAFPYGRENVRRRPGRGELETEGLGLMAQDMGHQCWGAPHLEIRHARW"
     gene            complement(10348..>12466)
                     /locus_tag="VGB76_06200"
     CDS             complement(10348..>12466)
                     /locus_tag="VGB76_06200"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0098527.1"
                     /translation="ADLPAGTFRLAAEVMNFLLTTPVAARAVEATVTILSRPNQPSHV
                     YEALVGALRHAVTWAKELLDLETLAGLSEFEHLSSHRDYLLQEIVEPCIYAAGETATP
                     EALARITQLYGESDSFKYCLYNVKSRSDFRPDVRELASRLLQGRFSLHEEIARRLGSG
                     SRRILVVQNLNDGQGDEIVRTVPLMQALLDFNPAIEIILMTRRVYLYATPRITVIPLK
                     DRDRITALLDQRFDAVIDFFEPTMLEVNRDPDLEPLIQDYVRRHRPFLFVSSTNGYNH
                     FVYEQVYVDSRPVARSLGLDRPRVKNTYETTLRLIAELGLPMRCGEDPPATDWVLAGL
                     SWNEAEAAWRELVRQNTEGRPVALLNPFGGAEPLKGYVERQMDKLSAMIRQLIREKFY
                     VVLLPNGTPWGAASLAAEAIRRLEPHEQAQVVVGPDPACGSDAAPHTVPGAPALTHPD
                     RVVRMVTYFIRFADLIVAVEGWMVHAAFCLGKKYRMLMMAYSYPDRWHPYAVTRHQQI
                     APGLLQCAYPSLEKESAALPILEQPRKFILLLLLREFGNAGDAQALELLRRAARSEDR
                     DLRQAAALSLGRLKTAEVEQELISLLQDPFRGVRADAASALLKMQEEQGTRPGDLPRE
                     YLLVLVASGHELRNWATIISVGEAARPALELALQDDDPVVRREALQVMRVLDFKAKLR
                     RRRPSLRTRLMRNRILRGLFGRG"
BASE COUNT         2547 a         3668 c         3646 g         2605 t
ORIGIN      
        1 ctagaagcag tctcataaat agcaccgcag cagaatcttg atgcagccga ggtgcacgaa
       61 gccgaggaag ttctcgacgt gataatcaaa gcgcgtcgcc acgcggcgga agttctggag
      121 ccacgcgttc agccgctcga ccttccaccg gcggtagcgc ctcaacttac gcccgtcctg
      181 cgtccgaggc ttcttccggt tcttgcggtg cggcgcgatc agctcgatgc cgcgcccggc
      241 caactccgcg tcgagcgggt cggagtcgta agccttgtcg ccgatcaagc gttcgggccg
      301 cccggcggta aaactctgaa ggagagtttc gccgacaagg gtgacttcat gcggcgaagc
      361 agacgccgcg tggacggcga gtggaagact agcattgtct gccaccacca tgagtttcgt
      421 gcctttgccc cgcttagtct taccgactcc gcgcccccct ttttcgcggt cacgaacgtc
      481 ccgtcgatga agcactcgga gaagtcaact tcgccgcgct cgcgcaggtc ttcggccagg
      541 gcttcgagca cgcgccgcag cgtgccgtcg cgcgaccact gctggaaccg gcggtggcag
      601 gtctggtacg gcgggaatct gtcgggtaag tcctgccacc gcgcgcccga cctcagaatc
      661 cagagcaccc cgttcaagac ctcgcgggtg tccctccacg gccggccgcg cttatcttcg
      721 cggcgctgcg gttctggtat cagcggggct aaaatagccc attgttcatc agtcagctct
      781 tcgcctcgtg ccatgcggaa atgctagcac gggtatttat gagaccgctt ctagtagaac
      841 aataagtatt aaatggccct tccgaggagg tactttcact tctcacgcac ccttctaccc
      901 ttcagttccg gatgccgtag gcgctggaaa ttttcgaaca gcacggaagg ggtggtgtgg
      961 tcatttgagg taaggaaggt cgtgaggagc cctacgccta tgcggccaca ccgaacaggc
     1021 ttgcgacaaa cttcgcatgc ccttgcgaat cacttcccgc taaccttctg acttgacctt
     1081 tgcgtatatg tttaccgctt caatgccttc gagagttcgc tccgccgtgt gaaagctctt
     1141 gaagcattgt gaggctcgca ccttcttctt gacgaaccgg tggtcctgct cgatcacatt
     1201 gttcaggtat ttcacgcgcc tgagcttgca gtccttcggc acaaccttct cctcctgtga
     1261 agcgctgaaa gcctcagggt aagccgcatt tttatcgacg ctgatcgaga atggcaggcg
     1321 gcggtgctcg gccctcatca tcttcttaaa gcagcgtttc gccgcggaaa catcacgttt
     1381 cgcgctgagc atgaattcta tggtctgccc ctccttgtct actgcgcggt acagatactt
     1441 acatgacttt ccgactttga tgtaggtctc atccacacgg tatgaggcgc cgctcatccg
     1501 cagatgttga cgaatgcgtt tattgatttc gggagcatac ctttgtaccc aacgaaacac
     1561 cgtcgagtgg tctacttcga gtccgcgctc gcgcatcatc tcttcgacat cgcggtaaga
     1621 tagctggtag cgacagtacc agcggacgca caacaggatg agcgacggct cgaagtgacg
     1681 ccacttgaac gaggacttat cttttttcaa gggtatgatc ccggaaaata atggccgaac
     1741 tgaagggcgg attatagctg ggaggcaatt cctgcaacac tacccttcgt ccttgcccac
     1801 ccgaccaggc aatccatatt atcctaagtg ctcgcacaaa attagatcga accattttgg
     1861 gatgtccagc gtttcaaatg caatggtttc gatcaaggat agttcaaact aaattagtct
     1921 gagtacttaa ttggattagg agctggcaac tatgctttcg cttctcgcac ggagccgttc
     1981 ccttcttcgc ggccgtggcg cacgcggcct gcgcgccttc gacagtctgc ggttctcggt
     2041 gctcgacctc aagcttggcg gacgcatgct ggtcaagcat cctgcgctca ccgtgatcgc
     2101 aacgattgcc gtagggttcg cggtcgccgt tggcactgtc gggttcgaga tcgcgcgaca
     2161 agcgctctgg ccgacgattc cgcttccgaa cggcgacgcc atcgttgcgt tacaaaactg
     2221 gaatgccgcc gaaagcgtgc ggatcgacgc atcgcgccgt gattacgagt tgtggcgcga
     2281 cggtctcagc acgatcaccg atcttagcgc cgtcgaggtg gaagaacgca gcatcgccgt
     2341 cggcgccggc cccggtcagc cggagaccgt cgccaacgtg accgcgtcga cctttgcgct
     2401 gacgcgcgtt ccggcgttga tggggcgcac gctgctggga gccgatgaac ggggcggcga
     2461 agaagaggtc gtggtggtcg gatacgactt ctggaagaac aagctggacg gcgcggcaga
     2521 cgccgtcggc cgcgtcattc gcatcagcgg cacgcctgtc acgatcgtcg gcgtgatgcc
     2581 gcgcggctac ggatttccac accggaacga cgtctggcga ccgttgcatc tcgagcagat
     2641 tcccgaggca tcgccgagcc tcgggtatgt ctttggccgc ctggcgccgg gccgtacgca
     2701 cgaggaagcg agcgcagagc tggcggtgat cggcgcccga gcagcgacga cgtttccgga
     2761 gacgaacaag aatgtgcgcc tgcaggtcct ctctctgccc gacgctgtca gtgatttgcc
     2821 agaagcggcg gcgctggtgc tggcgtcgat caacgtgttc ctcgtgctgt tggccgcgct
     2881 tttgtgcggc aacgtcggaa tgttgttgtt cgcccgcgcc gtcagtcgag agcgggagtt
     2941 cctagttcgc gccgtactcg gcgccagccg tgggcgcctc atcatgcaac tctttgccga
     3001 ggcgctcctg ctctgcgccg tcggcgcggt ggttggtctc ctggttgcgc gcttcgtgct
     3061 cgcgcgggct tggacggaga tcgaagtgca aatgggtccg ttgccgtttt ggatcgacac
     3121 ctcgctttcg acgacgacga ttctgtatgc cgttgggctg acgctgttcg cggcggcgat
     3181 cgccggcgtc gttcccgcgc tcaaagtgac gtcgggtggt gctagtggca ggctccaggc
     3241 cgcgtcaagc ggcggtggcg ggttacggtt cggcggcgtg tggaccgtgg tcatcgtcgc
     3301 gcagattgcg ttcacgacgc tgcttccgtg gccgctgctc ggcctgggag gggccatcgg
     3361 gggagacacg cccgcaggat tcccggccga agcatttctc acggccacgc tcgaaatcga
     3421 tcgctttgac ggcgtcgcgg cgagtggaga cacggcgccg gcggtgcgtg cggcgcggct
     3481 cgaagcacgc taccgcgcgc tcgccgaccg gctccgacag gacccgggcg tgctggacgt
     3541 cacgtacgcc gatcaaatgc cgcagttgat taccagtaat tttcgcgcga tcgagatgga
     3601 cccgggtccg gccgctgcgc ccggtgaaaa ctgtgtgggt tgcgactacg ccaaggtggt
     3661 gtccgtcgat ccgcgattct tcgacgtggt cggtgcaccc gtgatcagcg gccgggcgtt
     3721 gacgacggcc gacgcggaac accggacgcg tgccgtgctc gtgaacgagt atttcgtcga
     3781 tcaggtattg ggcggccgta atccaattgg ccgcaggttt cggttctcat cgactggcga
     3841 acccaccgcc gagtcgtggt atgagatcgt cggtgtcgtg cccgatctga gcgtaagcga
     3901 cactgggacc ccttacggcc gtcgcatcta tcagacgacg ctgccgcgtg agacggggcc
     3961 gcttcgagtc gccattcacg tgcgcggcga tccgcagcgt tttgcggtgc gtctccgcga
     4021 gctcgcgcag gcgaccgatc ccgcgctccg cgtgatcgat ccgaaaccgc tgtcccttct
     4081 ggaggatccc ggttccgcag gattgtcgta cgggttcgag ctcatggtca gcctgacgct
     4141 cgtgacgctg atgttgtcgc tcgccggtgt ctatgcggtg acggccttct ccgtcgccag
     4201 gcggacgcgg gagatcggcg tgcgcgtcgc gctcggcgcg cagccgctgc acatcctgac
     4261 taccgtcttc aagcgcccac tcattcaggt ggcgatgggc atcggcgtcg gtgcgatcct
     4321 ggggttcggt ctcgccaacg acgacctgtc gaagatgcat ctcgatgttc tcggcaagac
     4381 ggcggtcttc gcgctgtcga cgatcctgtg ctgtgcactc gcatgcatcg tcccggcgcg
     4441 acgggcgctt cgcattcagc cgacggaggc attgagggac gacggttgag ctcacgaccc
     4501 ccgcggtgtg gcgcgaacgg atgtgttgca agaattgtct atgagcttat aatccaccct
     4561 tcagctcagc catcactcat cgggaatcat cctcatgaag cagcctaagc acccgttcaa
     4621 gtgacgtcac ttcgagccgt cgctcatcct tttgtgcgtc cgctggtact gccgatactc
     4681 gctcagctat agagacctcg aggagatgat gcgagagcgc gggctgagcg tcgatcacgt
     4741 caccatcttc agatgggtcc agaagtacgc tcccgagata aacaggagga tgcgcccgca
     4801 cctgaggatg ggcggcacct catatcgact ggatgagaca tacgtcaaag tcagtaaaga
     4861 gtggaaatac ctttacaggg cggttgactc taaggggcag acgatcgaat tcatgccgag
     4921 cgcgaagcgc gacgtttccg cggcaaaacg gttctttaag aagatgatgc gggcagatca
     4981 ccggcggctg ccgttcacca tcggcacgga caagcacgct tcttaccctg aggcgttctc
     5041 cgcctcagtc aaagagaaag tcctgcccac tgattgtaag ctgcgccgag tgaaatacct
     5101 caacaacgtg atagaacaag accacagagc gatacgaagg gggtggcggg cgatgcaatg
     5161 cttccgctcc ttccacactg ccgagcggac actcgaaggc gtagaagcta tgcatgtgat
     5221 ggtgaagggg caggtcaaga ggttagacgg aagggactcg gtcgggcagg cgaagtttgt
     5281 cgcttcgctg ttcggaatcg ccgcttaacg aactgcactc taagaacttg tgcactggaa
     5341 aataatcttt tcaacgcatc ctgtaggaga tactctcaaa ttgccaggtg ctcgtagtag
     5401 agttctcgcc gcatcacttg aagaacctta gtggcagaac agttcctatc agtaattgag
     5461 ccccatttct caatactgac aataccatca agacgaatta acataggcgc ctcggaattc
     5521 gttaggtgtg tgactgcgac gcacgacctc ggacgaggga acgaaggcat catatttgag
     5581 aagacctgac aggcccccgg gctcggccgc cgcttcacag tgattcaaaa cagacgtgtt
     5641 ttaattgttt cccggcatgc gaactgttct attagtttct aacgcgttac ttctgctttt
     5701 attgattgtg gccgcgcgcc gcatgctcaa ctatcggtcc cggttgagga gactcgaact
     5761 gaactatagg tctcagctaa aggcaatcga agggacattt gactctgtca tcccgctagt
     5821 caggctggat gaatttcacg aggtgtttgc tcaaaccgga tcgggcttca acccggaaac
     5881 cgaagtgagt ttcattggat gctgggtgag aggggggacc agttgcttcg agagctgggt
     5941 gttatcggtt ctggcaaagc gggcaacgtc cctgtttgaa ttcggcacct gctcgggcaa
     6001 gacgacctat ctctgggcga agaattctcc ggccgaagcg agaatcatca ctttgacttt
     6061 aggcaaagac cagcacgcgg attatcttgc ggcgggagaa gataaccttt tggcgacccg
     6121 agcggcactg gacgaatcga cttttgacag gttcatttat tcgggcaccc cggtcgaaca
     6181 caaaatcacc cagctcttcg gcgacagcaa acgattcgac gaaacgcctt atctgaaaca
     6241 atgcgacctg atctttattg acggctcgca cgcatactct tacgtcaaga acgatacgga
     6301 gaaagcgttg cgcatgctga aggaaggtgg actccttctc tggcacgatt accgtgatcg
     6361 caacgtggaa accagaggcg tgcgtcgtta cctggatgag ttgagcgaga ccttgccgct
     6421 gattcgtttg tccgatacaa cgctcgtcgc ttatcgcaac ggtactaaca aagtcgcggc
     6481 ggactagaga gcggaaggcg ggacgcactt ctcagtaaac cggggagacc aaaagagagg
     6541 cgatggttct ttttatagcc tcggccgagt agtggtggcg taacaacgaa aacgcattct
     6601 ccgccagccg ctctcgcaaa acgcgatcag ccataagccg gacgcagcgt tcggcaaaga
     6661 cttcgggcgt gtttgcggtc aagacgtgta cgtcgtcgac ggcgtcaatg ccttccatgc
     6721 cgattgaggt gctgatgacc gcgcgacgat ggctgaaggc ttccagcacc ttgatgcgtg
     6781 tgccgcctcc cgcccgcaca ggcacgacca cggcggtgga gttctgatac cacggcgcga
     6841 cgtccgggac ctcgccgatc atccgcatgc cgtaatcgac cgcgatctgg cggagccgtg
     6901 ggccggctcc acttcccaca atattcacta cgaagtcaga ccgggtcagg cggcgaatgt
     6961 gcgggaggat ctcgcttccg aaataccgca ccgcatcttc gttcgggtag taaccaaggg
     7021 ttccgacgaa caggaaagtg aagacgccct cattcgcgtc gcgccgcccc acgtcaggaa
     7081 ggcgcaccgc gttgggcagg acgcacagct cactcccgtg gtcggcgtgc agcctcgcct
     7141 tgtcgccttc ggagcagacg taaacgcgct cgaattcccg aagcacttct tgctctaaag
     7201 cctcatagcg ccgggcctcc atctcatagt aagcggctcg cgggccgtct ttgttcaaac
     7261 ggcagagcgc ggctatacgg cggtgggtct cggattcgat gtcgtcaagg tcgagatgcc
     7321 cttcgggcgt cccggaagag acattaagga acggacgcgc gtaaggcatc atggagagcc
     7381 gaaacacgtg cactacgtca aagcgcgatc cgtggtaagg gcgccgcccg aatggcctgc
     7441 taagaaactg cggcagaaac gagaccctcg tgcgggcgaa ctgagacgga gtcgtcacga
     7501 ctgtgcgcag gcagagtctc tcgaacgcgg cgggcacacg agcagcaaac ggcgggtata
     7561 gcgaaacgac cagcagggag atcgaataat gctcggcgag ggcttcgaga accattcccg
     7621 cccgcatcgc caggccgttt ccggccagag ccgggaccac gggactgaga tacaggagtt
     7681 caggtttcga cgacattcag ctcattacgc cgcggcgaca ctgaaaagaa gggggcgccg
     7741 cttgttctct agtgttggtg caatacgaga tttactctcg caacccgagc cgatgctgca
     7801 cagttttgac gactgccgag acggtcgaag tcaatcggtc ggcaaagcca acgcccgcgc
     7861 gatccgcggc gctcatctgc cgcactttct ccgagaggcg aaggaatgaa tccttcgcca
     7921 tgacctccac tttgtcgagg aatatttgtc tcaggggctc tgtcttccag gccagatatg
     7981 agtcgtactc agattgattg cccgccgcga agatcaggta ctcggccagc tcgcgagggc
     8041 ctttgaattg cgccacgtta atgtagcaat gttctcccgg agcgaaatcg tcaatattcg
     8101 gcgcacccag gtaaacgggg accgagccga cgattagcgg gtcaaagaac ttctccgtca
     8161 cgtaatcctg ggagatcgag ttctcgaagg ccagcgtgaa tttataggcg gcgatagttt
     8221 ccagcttcgt ctctcgccgc ttgtcctcgg gaagcttctt attctgtagg gtcaggccgt
     8281 aggagtctac gggaaggcat tgcatcaact cctgcatgta cttgaacctg cctgacaaat
     8341 caatcccgtt ggaaacgaaa gcggccgccg gcgcgcttgc cgtctttagc gccggggcgc
     8401 ggagaagctc atctatggta tctgggccga agtaggcgac gggtacgtca gagtcccagc
     8461 gatacgtcat cgtaaggtcg aactgcctca tgtatttcgg atcgcacagc cgtggataaa
     8521 agcggtcgct ttcaaacgac caggccaccc atttttggcc gggccgcttc ctgagtccac
     8581 gcagttccgg cgccgtcggt atgtggaaga ccacgacgtc cgcatccctc agccactttg
     8641 gatcggaaat gatttcgtaa ccgggcgcgc aggccgaagt cggtgcaagc gacgcccccc
     8701 atttgaagtc gtagacgaga atcttaatgg tcgaagtatc gcccatcgag ctataaccgg
     8761 cctcttcaac attgctcatc cgaatagccg gctccacatc attgttccct tcttccgcat
     8821 ccttgcccac attttccggt cgttgaaagt cggctcattc ggcccgccac cgtcactcca
     8881 gatgtccgac ttgaaatctg ccagcgcgac aacactcggg gtgacgccgt acagatgcag
     8941 gccggttata taagtgaggc ccacaaagtc atccgcgggc agacggatcg gatacacatg
     9001 ctcgatcaat tttttcgccc cttgccgatt gagcaagtaa gccgaagtcc tattcgcaat
     9061 ccctctgaat cggcaaactc tatagtcttt gcagaccggc ccctccaggg gaatggcttt
     9121 cgcccacgta ttggcaaaat ttaccgcctc ccactccggc gggaatttcc ttctttgcag
     9181 aagcacgtgc aacaaatcct gtgttagaac gatgtcatct tcgaggatca aaacttcatc
     9241 gagattctcg tcaagcatct gctgatagat tttcgcatgt gtcagcgcgc aaccgatttc
     9301 acccgttgat aattcccgcc ttttaatttt caaagcgtgt ggtttggaat acttttgcag
     9361 ctcccctcgc gataattgtt tgccatcggt ggcattgatt atccggtaag acaaatccag
     9421 tgacgcaaat tgctcctgca tgaatcgtct tctctcagga gagcgcgcca gattggcaac
     9481 ccaaattggc ggcaatgcaa tggtgcaatc ttccttcttc atagcttcgc gctttaccac
     9541 ctggcatgcc gtatttccag gtgcggcgcg ccccagcact gatgccccat gtcctgcgcc
     9601 atcagcccca gcccttcggt ctcaagctcg ccacgcccgg gccgccgacg gacgttttcc
     9661 cggccgtaag gaaaggcagg aaagatcagc ccgtcccggt gcaggtcagc gcgcacgagc
     9721 agcatagtcc cgccgacggc atccagttcg accagttctc cttcccgacg caggtcgtcc
     9781 atgtgatacc gccccttgtc gcgccacgcg ttcctgtcaa aggtcgcgcc tccgaactcc
     9841 agcacgcaat gtgggtgaac gatctccttg ccggtcgcca gcaaccgttc gatgatgtcg
     9901 ggcgggtact cgataacgtc cacgtctaac cacacgaccc aatcctcgtc gtcgagggca
     9961 tgcatgagca gatgattgcg gctcctggcg agtaccgtgc gccgctcgac ctgaatcgct
    10021 tcctccccgc gatgaacgcc cgatggcacg tggtacccaa aatctctttt ccacagaccg
    10081 acgcgcctga attgcctccg cagcaccgga atgtgagcct gcaacgcccg ataggtggag
    10141 tcgttgctgt cgctttcaag cagacccacc gatattgatt tatgaggata ggtgagttgc
    10201 cgaaggcgtc ggcagtaccc ttgaatgaaa acggaggcgt tcttcaccgg cgtcaggatc
    10261 aatatccgat cccgaccttt gacggcgttc tttccgggcg ttatcaagtt ggagagcagt
    10321 tcgagaatag gcatcttgcg cttaccttca accccgtccg aagaggccgc gcagaatacg
    10381 attccgcatc aagcgcgtgc gcagcgaagg ccgtcgtcgg cgaagctttg ccttgaagtc
    10441 gaggactcgc ataacctgaa gcgcctccct gcgtacgaca ggatcatcgt cttgtaaagc
    10501 cagttccaac gcggggcgtg cggcctcccc gacgctaatt atggtggccc agtttctcaa
    10561 ctcatgccca ctcgccacca agaccagtag atactcgcgc ggaagatcgc cggggcgggt
    10621 accctgctct tcttgcatct tcagcagggc cgaggccgcg tccgctcgga ctccgcggaa
    10681 ggggtcctga agtagcgaaa ttaactcctg ctcgacctcc gcggtcttga gcctgcccaa
    10741 tgagagcgcg gcggcctggc ggagatcacg atcttcgctc ctcgccgccc tgcgcaacag
    10801 ttcaagagcc tgcgcgtcgc cggcattacc gaattcgcgc aggaggagaa gcaagatgaa
    10861 ttttcgcggc tgctccagaa ttggaagagc agcgctttct ttctctaacg aagggtaagc
    10921 gcactgtagc aatccgggcg cgatttgctg atggcgcgtt acggcgtacg ggtgccacct
    10981 gtcagggtag gagtatgcca tcatcaacat ccggtacttt ttgcccagac aaaaagcggc
    11041 gtgtaccatc cacccctcga ccgccacgat caggtccgcg aagcgaatga aataggtcac
    11101 catccgtacg acacggtccg gatgcgtgag ggccggagcg ccaggaaccg tgtgcggcgc
    11161 tgcatcggaa ccgcaggcag gatcaggtcc cacaactacc tgtgcctgct cgtgcggctc
    11221 aagacgcctg atggcttcgg cggcgagtga ggccgcgccc cagggcgtcc cgttgggcag
    11281 aagaacgacg tagaacttct ctcggatcaa ctggcgaatc atcgccgaga gcttatccat
    11341 ctgccgctcg acataccctt tcagaggctc cgcgccgccg aaggggttca acagcgcgac
    11401 cggacgccct tcggtattct gccggacgag ttcgcgccat gctgcttcgg cctcattcca
    11461 ggagaggccg gcaaggaccc aatccgtcgc cggagggtct tcgccgcacc gcatgggaag
    11521 ccccaattcg gcgatcaagc gcagggtggt ctcataggta tttttcacgc gaggccggtc
    11581 caatcctaga gaccgggcca cgggacgcga gtctacataa acctgctcgt agacgaagtg
    11641 attgtacccg ttggttgagc tgacaaagag aaacggtcgg tgtcgccgaa cataatcctg
    11701 aataagcggc tcaaggtccg ggtcgcgatt aacctcaagc atggtcggct cgaagaagtc
    11761 aatcaccgcg tcaaagcgtt gatcgagcag ggccgtaatg cggtctcgat cttttaatgg
    11821 aataactgtg atccggggcg tggcgtacag gtagacgcgg cgcgtcatga gaatgatctc
    11881 aatcgcgggg ttgaaatcga gcagggcttg catgagcggg acggtgcgta cgatctcatc
    11941 accctggccg tcgtttaagt tttgcaccac cagaatgcgt cggctcccgc tgcccagtcg
    12001 gcgcgcgatc tcctcgtgga gcgaaaaacg gccttgcaat aatctgctcg ccaactcacg
    12061 tacgtccggc cgaaaatcgc ttcgggactt gacgttataa agacagtact tgaaagagtc
    12121 ggactcgcca taaagctgag taatccgggc aagcgcctcc ggagtggccg tctcgcctgc
    12181 cgcataaatg caaggctcga cgatctcttg aagtagataa tcacgatgcg acgacaggtg
    12241 ttcaaattcc gacaggccag ccagggtctc caggtcgagg agctccttcg cccaggtcac
    12301 ggcgtgacgc agggctccca ccagtgcttc ataaacatgt gagggctgat tcggccggga
    12361 taaaatggtg actgtcgctt cgaccgcgcg tgcggcaact ggtgtagtca gcaggaaatt
    12421 catgacctct gccgcgaggc gaaatgtgcc cgcgggcaag tcggcc
//
LOCUS       DASWNS010000181         7720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_112123, whole genome shotgun sequence.
ACCESSION   DASWNS010000181 DASWNS010000000
VERSION     DASWNS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112123"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VGB76_06205"
     CDS             <1..184
                     /locus_tag="VGB76_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098528.1"
                     /translation="GMVSSYSPAALVVAEHDRDWQRMINVVRYEGEHLVLYHNDRRRF
                     QQTADHARGGAHPILR"
     gene            complement(358..1164)
                     /locus_tag="VGB76_06210"
     CDS             complement(358..1164)
                     /locus_tag="VGB76_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098529.1"
                     /translation="MFSYKLRIIAVVALAFSFTSIAAAQDGGNTQPAPQTDNSISHTS
                     FKNRVFEVKHRDPDTLLPVLRLLTSGHKGSGVSANRAFKTITVRDFPENITSVEEALR
                     RLDVPEAARPEIEFRMHVLIASNLAGGANQYPADLRDAVGQLQTTLNFKSYYLLTSII
                     QRAKDRADFAPNIIHGEGVAQLALPGENAPRDFTYHFQANSLTLNTTSAGVTTVQLGS
                     FRFRLDGGDVGGRAVVSSDVGLRDGEKVIVGTAGLKDKALILVMTAKLIK"
     gene            complement(1204..2067)
                     /locus_tag="VGB76_06215"
     CDS             complement(1204..2067)
                     /locus_tag="VGB76_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0098530.1"
                     /translation="MMRCTRVEKFLPLYVAGDLAGHRAERRVANHLATCERCSDAATE
                     YDASRDLVRAATLPPDFDAAFYEEIRRTVLAEITRGRTRLAPPASHGFASLFNARFAY
                     AASLALVVAALAALSLQGYIGRKSEEALRPHVIANVNRESDGTAAATSTPVSNQARDG
                     VRQSSSPVRMTAREANQRRAQAAKSSSMQRRADGGDAQNERPRSVNLAKHAPVTGLLY
                     QDPARKSLSPRVGVAWDLSGSDTTSLRRHSPATDDAPEVSRIEIQTADPNIRIIWLSP
                     KPDDAVAQPLK"
     gene            complement(2070..2780)
                     /locus_tag="VGB76_06220"
     CDS             complement(2070..2780)
                     /locus_tag="VGB76_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0098531.1"
                     /translation="MATETTHLAALMTAMMAAGDFQTPADASEAADARKQFALLVARA
                     RAGDATAFERIMLDTERKVVATAWRMLGNREDARDAAQEVYLRVYKYLDRFKPEQDFN
                     GWLYRITINVCRDASRKKRGATSGGGGSGGGEPFAARSNEHERGVAAESVSLEANAEE
                     NAIRAQQRSILMSALETLPEKERAALVLRDLEGLTTEEVARVLRSRPVTVRSQISSAR
                     TKLKIHCARLLKKGGRPA"
     gene            3086..4288
                     /locus_tag="VGB76_06225"
     CDS             3086..4288
                     /locus_tag="VGB76_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY0098532.1"
                     /translation="MRRIDLKKAQVARSSTIRDINRQIVLNYVRDREPISRAEIARET
                     ALQRSTISTIVEDLKSDGLIEEIGEGESTGGRRPTLLRLRAAGAMAIGVDITPTCTTI
                     ATSDLGGRVLEQEEFPHIEQPERLYARVIESVRRLAAKAGERKIEGVGVSLPGLVDPD
                     SGRAVYIPWFNWRDWPIADEISAATGLDVIVDNDANAAALAALWFGRQELREARDFIM
                     VLVAEGIGTGIVVDGQIYRGERGGAGEFGHMIVGERAPVACSCGNHDCWEAFASGRAA
                     VARYLQNGGTSVDGAERIDLAQVVDRALAGEARAIHALTETAHYLGIGISNLIVGLSP
                     EAVVVGGVIARAWPLVSGALNETIERSVRRGLPSAHIMPSILGDHPTLMGAISLVLAR
                     KFASVAGA"
     gene            4440..5771
                     /locus_tag="VGB76_06230"
     CDS             4440..5771
                     /locus_tag="VGB76_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0098533.1"
                     /translation="MNQRLTLSGLHFSSASLIVAALVISVALVSSCRTKREAEGAIKM
                     HAAQLTNTLTPAERAEIFEIVWRTVNETYYDPAFHGVDWQAVRERYRPRMEAAPNDQE
                     FYAQFEVMLAELRDAHTSFNAPPPPGAPPEGQSGNLGLSLVEIEGQTIVSEVEADLSA
                     ERAGVRPGMLLRTVNGRPVEELYALIRANFAGTSSERAFAVLQRRAILYGGFLPKPRT
                     LGLSNLDNSTFEVTLAERQPATTAPRVEARRLGSGFGYIKFDQWKPPADARFKEELSK
                     LSETPGLVIDLRANGGGQTDVLLNIASNFFAAPTYTGGFRRRDGTLDRYLTHAPTRLY
                     KQPVVILIDERSASASETFAIFMQESRRAYVVGRQSNGALLNTRIQKIKGGGTLRVSI
                     RAYVSPDGRVPEGTGVVPDKIVPLTLADLRQGRDAALEAAEAYLNAHTGEK"
     gene            complement(5886..6332)
                     /locus_tag="VGB76_06235"
     CDS             complement(5886..6332)
                     /locus_tag="VGB76_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0098534.1"
                     /translation="MKPDFTVASASDVETLIELMREFYAFDHIAFDEGAARRALGQIL
                     RDRRFGVIHLLSVDGAAAGYLVVTFGFSLEFGGRDAFVDELYLRENFRGRGIGKASLE
                     LAGTVCLQEGIHALHLEVERANTKAQGIYRQAGFRDHDRYLLTKWL"
     gene            complement(6340..>7720)
                     /locus_tag="VGB76_06240"
     CDS             complement(6340..>7720)
                     /locus_tag="VGB76_06240"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEY0098535.1"
                     /translation="VGDYDNDGHLDLYVTAFGPNVLYRNNGDGTFTDVAEKLGVARKG
                     WSTGVTFGDYDRDGRLDLFVPGYVEFDVKNLPPSPSDAGKPGGVGQNFCQFRGVPVMC
                     GPRGLKGEGDTLYHQKADGTFEDVSTKTGTHDPQGYYGFSSAFVHANEDDLLDLIVVN
                     DSTPKQLYINKGGGAFEEVGYESGVALNENGREQAGMGLAVGDYDNDGRVDFYITNFS
                     DDSNTLYHNDGEGSFTDVTFQAGHGEPTIPFLGWGTSFLDYDNDGWKDVFVANGHVYP
                     SVDQHQWGTTWAQQSLLFRNIPNPRNAKSDPNDRVFERVGAAPGSGLAAAFPARGLAI
                     ADLDGDGRLDAVLNNCDAPPTVLRNVTDTKNHWLILRLSADTTKKNPRDATGALVYAT
                     TGAVRQRLDLISGAGYASQNDPCLHIGLGAATKLDKLEVRWPDGTLENVSVPGIDRVL
                     TITQGKGAK"
BASE COUNT         1395 a         2391 c         2435 g         1499 t
ORIGIN      
        1 cgggatggtt tcttcctaca gtcccgccgc gctcgtcgtc gccgaacacg accgcgactg
       61 gcagcgcatg atcaacgtcg tccgctacga aggcgaacac ctcgtcctct atcacaacga
      121 ccgccgacgc tttcaacaaa ccgccgacca cgcgcgcggc ggcgcacacc cgatattgag
      181 ataagacaaa cggtgaatgc ggaacgcgga acgatgaagg agcagccaga aaacagaaca
      241 caggagtcag aagcaacaga cgcgcctgct ttattctgac tcctgtattc tgttttctgt
      301 cttctgtttt ctgactcctg cttttcattc cttgttcatc gttccgcctt catcgtttta
      361 cttgatcagt ttcgccgtca tgacgaggat gagggctttg tctttgagtc cggcggtgcc
      421 gacgatgacc ttctcgccgt cgcgcaagcc cacgtcgctg ctcacgacgg ctctgccgcc
      481 cacgtcgcct ccgtccagac gaaaccggaa actaccgagt tgcacggtcg tgactcctgc
      541 cgaagtcgtg ttgagcgtca gcgagttggc ctgaaagtgg taggtgaaat cgcgcggggc
      601 attctcgccc ggcagggcga gttgggcgac gccttcgccg tgaatgatgt tgggcgcgaa
      661 gtcggcgcga tccttggcgc gctggatgat cgaagtcagc agataataac ttttgaaatt
      721 gagcgtcgtt tgcaactgcc cgacggcgtc gcgcagatca gccgggtact gatttgcgcc
      781 gcccgccaga ttcgaggcga tgagtacgtg catgcgaaac tcgatttcgg ggcgcgcggc
      841 ttcgggcacg tcgagccgtc tgagagcttc ttcgacggac gtgatgtttt ccgggaagtc
      901 gcgcacggtg atcgtcttga aggcgcgatt agccgacacg cccgatcctt tgtgcccgct
      961 cgtcaacagg cgcagaaccg gaagcagcgt gtcagggtcg cgatgcttca cttcaaagac
     1021 gcggtttttg aaactcgtat ggctgatgga gttgtccgtc tgcggcgcgg gctgagtgtt
     1081 gccgccgtcc tgcgccgcag cgatagacgt gaagctgaat gccagcgcga cgacggcgat
     1141 gatcctgagt ttgtatgaaa acataatttc tcctgttaac tgtcgaatcg gaaattgaac
     1201 tttctacttt aagggttgcg ccacagcatc gtccggttta ggcgagagcc agatgatgcg
     1261 tatgttgggg tcggcggttt gaatctcgat gcgcgagact tccggcgcat catcggtcgc
     1321 cgggctgtgg cggcgcagag aagtcgtatc ggagccggat aagtcccacg cgacgccgac
     1381 acgcggcgac aggctcttgc gcgccgggtc ttgatagagc agtccggtta ccggtgcgtg
     1441 ctttgctaga ttcacacttc gcggcctctc attttgcgcg tcgccgccgt ctgcgcgtcg
     1501 ctgcattgat gatgacttcg ccgcctgcgc tcttcgctga ttagcttccc gcgccgtcat
     1561 gcgaaccgga gatgaacttt gccgcacgcc gtctcttgct tgatttgaga cgggcgtgga
     1621 cgttgccgcc gctgtcccgt cgctttctct attcacgtta gcgatcacat gcgggcgcaa
     1681 ggcttcttct gactttcgcc cgatgtagcc ttgaagcgag agcgcggcga gggcggcgac
     1741 gaccagagcc aacgaggccg cataggcgaa gcgcgcgttg aagagtgaag cgaagccgtg
     1801 ggaggcgggc ggtgcaagcc gcgtgcgccc gcgtgtgatc tctgcgagca ctgtgcgtcg
     1861 aatctcttca tagaaagcgg cgtcgaaatc gggcgggagc gtcgccgcgc gtacgaggtc
     1921 gcggctcgcg tcatactctg tggccgcgtc gctgcatctt tcgcacgtcg ccagatggtt
     1981 tgcgacgcgg cgttcggcgc ggtgtcccgc gagatcgccc gcgacgtaga gcggcagaaa
     2041 cttttcaacg cgtgtgcatc tcatcatctt taagcgggtc gccctccttt tttgagcagg
     2101 cgtgcgcaat gaatcttgag cttcgtgcgc gccgaactga tctgcgagcg cacggtgacg
     2161 ggacgcgaac ggagcacccg cgccacctct tcggtcgtca agccttcgag atcgcgcagg
     2221 acgagcgcgg ctcgctcttt ctccggcaga gtctccagcg cgctcatcag gatcgagcgt
     2281 tgctgcgcgc ggatggcatt ctcttccgcg ttcgcttcga gcgacacgct ctccgccgcg
     2341 acgccgcgtt cgtgctcatt cgaccgcgcg gcaaaaggtt cgccaccgcc actaccgccg
     2401 ccgccgctcg tcgccccgcg cttcttgcgc gacgcatcgc gacagacgtt gatcgtgatg
     2461 cgatagagcc agccgttgaa atcctgttcg ggcttgaagc ggtcgagata tttatagacg
     2521 cgcaggtaaa cctcctgcgc cgcgtcgcgc gcatcttcac gattgccgag catgcgccac
     2581 gccgtagcca ctaccttgcg ctccgtgtcg agcatgatgc gttcgaaggc ggtcgcatca
     2641 ccggcgcgtg cgcgtgcgac gaggagcgcg aactgtttcc gcgcgtcggc ggcctcgctc
     2701 gcgtcggcgg gcgtctggaa gtcgcccgcc gccatcatgg ccgtcatcaa cgccgccaga
     2761 tgtgttgtct cagttgccat ctctgctctc tctcaagtcg tcccgcacat ctaacactac
     2821 tcccgaccgc gcgagatcgt tgataagaat ctaacccgag attgacacgc cggacggtgg
     2881 ctgtttgttc cacgcagatg gacccggagc cgcgacgcgc ccggagtttg aaaacggaaa
     2941 taagttgacg cgcgccggac aaaccactac aagatagttc gcgttccgca ggcggtcgac
     3001 tccgccaccg caaaaggacg cgccgcccgc aaccttgctc ccctgcgaac ggaaggattc
     3061 agctttaagt tgatcgagtt gattcatgcg acgcattgat ctgaaaaaag cgcaggtcgc
     3121 gcgttcgagc accatccgcg acatcaaccg gcagatcgtg ctcaactacg tgcgcgaccg
     3181 cgaaccgatc tcacgcgcgg agatcgcgcg tgagacggcc ttgcagcgtt cgaccatctc
     3241 aaccatcgtc gaagatttga agtcggacgg gctgatcgaa gagatcggcg agggcgaatc
     3301 gacgggcggg cgcaggccga cactgctgcg cctgcgcgcg gcgggcgcga tggcaatcgg
     3361 cgtggacatc acgccgacct gcaccacgat tgccacgagc gatctcggcg ggcgcgtgct
     3421 cgaacaagag gagtttccgc acatcgaaca gccggagcgt ctgtatgcgc gcgtgatcga
     3481 gagcgtgcgt cgtctggcgg cgaaggcggg cgagcgaaag atcgaaggcg tcggcgtgag
     3541 cctgcccggt ctggttgatc ccgatagcgg gcgcgcggtc tatatcccct ggttcaactg
     3601 gcgcgactgg ccgattgcag acgagatttc cgccgccacc ggactcgacg tgatcgtgga
     3661 taacgacgcc aatgcggcgg ctctggccgc gctctggttc gggcggcagg agttgcgcga
     3721 ggcgcgcgac ttcatcatgg tgctcgtggc cgaaggcatc ggcacaggca tcgtcgttga
     3781 cgggcaaatc tacaggggcg agcgcggcgg cgcgggcgag ttcggccaca tgatcgtggg
     3841 cgagcgcgca cccgtagcct gttcgtgcgg gaatcacgat tgctgggaag ccttcgcctc
     3901 cgggcgcgcg gcggtcgcac gctatctgca aaacggcggc acgagtgttg acggcgcaga
     3961 gcggatagat ttggcgcagg tagtagaccg cgcgctcgcg ggcgaggcgc gcgccattca
     4021 cgccctcacc gaaaccgcac actatctcgg catcggcatc tccaacctga tcgtcggact
     4081 cagccccgaa gcggtcgtcg tcggcggcgt gatcgcgcgc gcgtggccgc tcgtctccgg
     4141 cgcgctcaac gaaaccatcg agcgcagcgt gcggcgcggc ctaccctcgg cgcacatcat
     4201 gccctccatc ctcggcgacc acccgacgct catgggcgcg atcagcctcg tcctcgcgcg
     4261 caaattcgcc tccgtcgccg gggcataata aagataaaac taaagacccc cgcgcggcgt
     4321 aacctgagtt gcgactgcga ctgcgactgc aacggttctc gctcgaacgc gtctactctt
     4381 ttttgtgtga aggccgacgc ttcgcgtcgc gagcatcgag atgaagagat gagttgaagg
     4441 tgaaccagcg tctaacttta tccggcctgc atttttcgtc ggcgtcgtta atcgtcgccg
     4501 cgctcgtgat ctcagtcgcg cttgtctctt cgtgtcgcac gaagcgtgag gcggaaggcg
     4561 cgatcaagat gcacgcggcg cagttgacga acacgctgac gcctgccgag cgcgcggaga
     4621 ttttcgagat cgtctggcgc acagtcaacg agacgtatta cgacccggcc tttcacgggg
     4681 tcgattggca ggcggtgcgc gagcgatacc ggccacgcat ggaggccgcg ccgaacgatc
     4741 aggagttcta cgcgcagttt gaagtgatgc tcgcagagtt gcgcgacgcg cacacgagtt
     4801 tcaacgcgcc gccaccaccg ggcgcgccac ccgaagggca atcgggcaat ctcggtctgt
     4861 cgctcgtaga gatcgagggg cagacaatcg tgtcggaggt ggaggcggat ttgagcgcgg
     4921 agcgcgcggg cgtgcgtccg ggcatgctcc tgcggacggt caatggccgc cccgtcgaag
     4981 agttgtacgc cttgattcgc gctaacttcg cgggcacctc ttcggagcgc gccttcgcgg
     5041 tcttgcagcg gcgcgcgatt ctttacggcg gattcctgcc gaagccgcgc acgctcggcc
     5101 tctccaatct cgacaactcg acgtttgaag tgacgctcgc agagcgacag ccggcgacaa
     5161 ccgcgccgcg cgtcgaagcg cgccgcctcg gttcaggttt cggctatatc aagtttgacc
     5221 aatggaaacc gcctgccgat gcgcgcttca aagaagagtt gtcgaagttg tcggagacgc
     5281 ccggcctcgt catagacctg cgcgcgaacg gcggcggcca gaccgacgtg ctgctcaaca
     5341 tcgccagcaa cttcttcgcc gcgccgacct acacaggagg cttccgccgc cgcgacggca
     5401 cgctcgaccg ctacctgacg cacgccccca cacgcctcta caaacagccc gtcgtgatac
     5461 tcatagacga gcgttcggcc agtgcgtccg agactttcgc gatcttcatg caggagagca
     5521 ggcgagccta cgtcgtcggc aggcaatcga acggcgcgct gctcaacacg agaatccaga
     5581 agattaaagg cggcggcacg ctgcgcgtca gcattcgcgc ctacgtctcg ccggacgggc
     5641 gcgtcccgga aggcacgggc gtcgtgcccg acaagatcgt gcctttgacg ttggccgatt
     5701 tacgacaggg gcgagacgcc gcgctcgaag cggcggaagc gtatctcaac gctcacacag
     5761 gtgagaagta agcactcacg acagagacac agaggacgca aagggggcgc agagagttga
     5821 atcttctctg cgccctcttt gtgttctcta tggcgaaggt ttcaatttct tacaagctta
     5881 acctgctaca accatttcgt cagcaggtaa cgatcatggt cgcggaagcc cgcttggcgg
     5941 tagatgccct gcgctttcgt gttggcgcgt tcgacttcga ggtggagagc gtggatgcct
     6001 tcctgtaggc agaccgtacc ggccaactca aggctcgctt tcccgatgcc gcgcccgcga
     6061 aaattctctc gcaggtagag ttcgtcaacg aaggcgtcgc gcccgccgaa ttccagactg
     6121 aaaccgaaag tgaccacgag ataacccgcc gccgctccgt ccacgctgag caaatgaatg
     6181 accccgaagc ggcggtcgcg caaaatttgc cccaacgcac ggcgcgccgc tccttcgtcg
     6241 aaagcgatgt ggtcgaacgc gtaaaactcc cgcatcaact cgatgagcgt ttcaacgtcc
     6301 gatgcgcttg cgacggtaaa gtcgggtttc attggaagct cactttgcgc ccttgccctg
     6361 cgtgatcgtc aggacgcgat caatgccggg gacgctcacg ttttcgagcg tgccgtcagg
     6421 ccagcgcact tcgagcttgt ccagttttgt cgccgcgccg agtccgatgt ggaggcacgg
     6481 atcgttttgc gaagcgtagc ccgcgccgct gatcagatcg agacgctggc gcaccgcgcc
     6541 ggtggtcgcg tagacgagcg cgcccgtcgc atcgcgcggg ttcttcttcg tcgtgtcggc
     6601 ggagagccgg aggatgagcc agtggttctt cgtatccgtg acgttgcgca ggactgtggg
     6661 cggggcgtcg cagttgttga gcacggcgtc gagccgcccg tcgccgtcga ggtctgcgat
     6721 ggcgaggccg cgcgcgggga aggccgccgc cagcccgctt cccggcgcag ccccgacgcg
     6781 ctcgaagacg cggtcgttcg ggtcgctctt cgcgttacgc ggattcggga tgttgcggaa
     6841 gagaagcgac tgttgcgccc acgtcgtgcc ccactggtgt tggtctaccg acgggtagac
     6901 gtgaccgttg gcgacgaaca cgtctttcca accgtcgttg tcgtagtcga gaaacgacgt
     6961 gccccaaccg agaaagggaa tggtcggctc gccgtgcccg gcctgaaaag tcacgtcggt
     7021 gaaactgcct tcgccgtcgt tgtgatagag cgtgttggag tcgtcggaaa agttcgtgat
     7081 gtagaaatcg acgcgcccgt cgttgtcgta atcgccgacg gccaatccca tgcccgcctg
     7141 ctcgcgcccg ttttcgttca gggcgacgcc cgattcgtag ccgacctctt caaacgcgcc
     7201 gccgcccttg ttgatgtaga gttgcttcgg cgtggagtcg ttgacgacga tcaggtcgag
     7261 caggtcgtct tcgttcgcgt ggacgaaggc ggaggagaaa ccgtagtagc cttgcgggtc
     7321 gtgcgtgccc gtcttcgtgc tcacgtcttc aaacgtgccg tccgcctttt gatgatagag
     7381 cgtgtcgcct tcgcctttga ggccgcgcgg cccgcacatg acgggcacgc cgcgaaactg
     7441 gcagaagttc tgcccgacgc cgccgggctt gcccgcgtcc gaaggactcg gcggcagatt
     7501 cttcacgtcg aactccacgt agccgggcac gaagagatcg agccgcccgt cgcggtcgta
     7561 atcgccgaag gtcacgcccg ttgaccagcc cttgcgcgcg acgcccagtt tttcggccac
     7621 gtccgtaaac gtgccgtcgc cgttgttgcg atagagcacg ttcgggccga aggcggtcac
     7681 gtacagatcg agatgaccgt cgttgtcgta gtcgccgacc
//
LOCUS       DASWNS010000182         4984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_85578, whole genome shotgun sequence.
ACCESSION   DASWNS010000182 DASWNS010000000
VERSION     DASWNS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85578"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VGB76_06245"
     CDS             <1..409
                     /locus_tag="VGB76_06245"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HEY0098536.1"
                     /translation="YRIAADTLESELLLHGATITPEARESLERKIEQFKQTAARYESE
                     PDPKAPNDPLRGEGKKELSARAKDFADKRDRAQRQDPNFDFAEALFQIAIVLASVAIL
                     ATSRLIFKISVGVGILATLLMLNGYFLFMDLPL"
     gene            457..1518
                     /locus_tag="VGB76_06250"
     CDS             457..1518
                     /locus_tag="VGB76_06250"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0098537.1"
                     /translation="MKKTSLLSALALMLLVGVQARAESRVDSFGWGQATQAAGQSDVA
                     ADSPELKEAAQQTAAAVKFYRERKFDDALTAAKRALELREKVLPEDHALIKSSLNNLG
                     QIYLVKENYSDALKVFLRLEAAYEKSLPPGHENYDLLVRRLALIYFYLGKDSEAEKRY
                     RQLLAQRETARGAHHPQVAEALFAYASFYQFRGKEKEAEPLYARAIAIWEKQKDSDQT
                     LYMTALERYACMLRKSDRESEAETLETRARESISKEYDSKLAESVKGGVLNGKAISKP
                     PPGYPVEAKNQRQSGTVVVNLLVDETGRVLQACAISGPKLLRAASERAAYAARFSPTL
                     LGGVPVKVRGIITYNYVLQ"
     gene            1578..2027
                     /gene="dtd"
                     /locus_tag="VGB76_06255"
     CDS             1578..2027
                     /gene="dtd"
                     /locus_tag="VGB76_06255"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY0098538.1"
                     /translation="MRAVLQRVTRASVEVEGESVGEIDAGLVVLLGIAQDDTEEDARY
                     LSEKIVSLRIFEDAAGRMNLSVVETGGALLVVSQFTLYGDVRRGRRPSWSEAAAPEVA
                     ALLYEYFLSEVRKTGLRVETGSFRRMMKVELVNDGPVTILLDSRKLF"
     gene            2160..2831
                     /locus_tag="VGB76_06260"
     CDS             2160..2831
                     /locus_tag="VGB76_06260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0098539.1"
                     /translation="MNRNFRRWATALLCLSLASLALACGAASNNSNAGTNAPAEANVK
                     TGVKPEPDAEAAIIETDYGRIVLELYPNVAPQMVARFKKLVGEGFYNGTAIHRINPDL
                     GIIQGGDPNSKDNNPANDGMGNSGEPNVPAEFSDILYERGIVGAARGGGDINSANCQF
                     FITLKRQEAFEQPGNRYTVFGRVIEGINNADIISTAPVTDGTEHPADKILVKSITLAP
                     RANFK"
     gene            complement(2973..3371)
                     /locus_tag="VGB76_06265"
     CDS             complement(2973..3371)
                     /locus_tag="VGB76_06265"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HEY0098540.1"
                     /translation="MPKKRPEDFEYLSNDPDFVPDPEIEQFMEEEIARAPKDPEQLAG
                     RLRQNTAASPRDAGGDLDASWEDVNDSGTEVVSGDNPTPDQSDVEENAHAVGVNFEDN
                     EELEFIDKIDRRDRDRFELDPRSKTEDNSI"
     gene            3728..>4984
                     /locus_tag="VGB76_06270"
     CDS             3728..>4984
                     /locus_tag="VGB76_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY0098541.1"
                     /translation="MTHNLFNSLQQFDAGQFYSLPQLEREGVGPVSKLPVSLRIVLES
                     LLRNFDGKTVEEEDIRTLANWQPNAERTDEIPFIVARVLLQDFTGVPLLVDLAAMRSA
                     VARMGRDPKIIEPLVPVDLVIDHSVQVDAWSTPDALQKNMETEFKRNNSRYQFLKWGT
                     QAFESFQVVPPGIGIVHQVNLEYLAQGVIERNGVYLPDTLVGTDSHTTMINGLGIVAY
                     GVGGIEAEAGMLGQPVYFLTPDVVGVNMTGALRDGVTATDLVLRVTEILRAAKVVGKF
                     VEFHGEGAASIPAYDRATIANMAPEYGATVGFFPVDEKTLEYFRSTGRSEERVNAIRS
                     YYEAQGMFGIPRAGDCQYSQLIELNLAEIEPSVSGPKRPQDRIELSDLKERFNELLQK
                     PVGENGYNKQKEELDARFPVNLDAITT"
BASE COUNT         1067 a         1418 c         1506 g          993 t
ORIGIN      
        1 ctatcgcatc gcagccgata cgctggagag cgaactgcta ctgcacggcg cgacgatcac
       61 gccggaagcg cgcgagagtc tggaacggaa gatcgaacag ttcaaacaga ccgccgcgcg
      121 ttacgagagc gaacccgacc cgaaagctcc gaacgatccg ctgcgcggcg aaggcaaaaa
      181 ggagttgtcc gcgcgcgcca aagacttcgc agacaagcgc gaccgcgcgc aacgacaaga
      241 cccgaacttc gatttcgcgg aggctctctt ccagatcgca atcgtgctgg cgtcggtggc
      301 gattctcgcc acgtcgcgtc tgatctttaa aatttccgtc ggcgtcggca tcctcgccac
      361 cctgctgatg ctcaacggct actttctgtt catggatttg cctctttgaa accggggaaa
      421 agagataaag cgatcaactc gcgtccggga ggaaagatga agaaaacgtc tcttttgtcg
      481 gcgctcgctt tgatgctttt agtcggcgtt caggcgcgtg cagagagccg tgtggactca
      541 ttcgggtggg ggcaggcgac gcaggcggcg gggcagagcg atgtcgccgc agactcgcct
      601 gagttgaaag aggccgccca acagacagcg gctgccgtca agttttatcg tgagcgtaag
      661 tttgacgacg cgttgacagc cgcaaagcgc gcactcgaac tccgcgagaa agtcttgccg
      721 gaagatcatg cgctcattaa gagttcgctc aacaacctcg gtcagattta tctcgtgaag
      781 gagaactact ccgatgcctt gaaagttttc cttcggctgg aagccgccta cgaaaagagc
      841 ctgccgcccg gacatgaaaa ttacgacctg ctcgtccgca ggcttgccct gatctatttc
      901 tatctcggca aagactccga ggccgagaaa cgctaccggc agttactcgc tcagcgggag
      961 actgcgcgcg gcgcgcatca tccgcaagtg gcggaagccc tcttcgccta cgcctctttc
     1021 tatcaattta gaggaaagga gaaagaggcc gagccgcttt acgcgcgggc gattgcgatc
     1081 tgggaaaagc agaaagattc ggaccaaacg ctctacatga ctgcgcttga gcgttatgcc
     1141 tgcatgttgc gcaagagcga ccgggagagt gaagccgaaa cgctggagac gcgcgccaga
     1201 gagagcatca gcaaggagta cgacagcaaa cttgcggagt cggtcaaggg cggagttctg
     1261 aacggcaagg cgatctcgaa gcccccgccc ggttatccgg ttgaggcgaa aaatcagaga
     1321 cagagcggga cggtggtagt aaaccttctg gtggacgaga caggaagagt gcttcaggcg
     1381 tgcgccatca gtggtccgaa actgttaagg gctgcctccg agcgggcggc ctacgcggcg
     1441 cggttttcac ccacgctgct cggcggggtg ccggtcaagg taagaggcat catcacttat
     1501 aactacgtcc ttcaatgatg cctgctccaa ctgccagcgg gatgacgggc gaagcgcagg
     1561 aacggattga agagtagatg cgtgcggttt tgcagcgggt gacgcgggct tccgtggagg
     1621 tcgaaggaga aagcgtcggc gagatcgacg cgggcttggt cgtgttgctc ggcatcgcgc
     1681 aggacgacac ggaagaggac gcgcgttacc tctcggaaaa gatcgtctcg ttgcgcattt
     1741 ttgaagacgc ggcggggcgt atgaatttgt cggtcgtgga gacgggcggg gctttgctcg
     1801 tcgtctcaca gtttactctg tacggcgacg tgcggcgcgg gcgcagaccg tcctggagcg
     1861 aggccgccgc gccggaagtg gccgcgctgc tctatgaata tttcttgagc gaagtgcgca
     1921 agacagggtt gcgcgtcgag acgggaagtt ttcgccggat gatgaaggtc gaactcgtga
     1981 acgacggccc ggtgaccatc ctgctcgaca gccgcaagtt gttttaagtg cgggcgggtc
     2041 ttaacgcaga gcggcgaagc cagctactcc gtgcgcacgc ttcgcgcctt cgcctctttg
     2101 tggttatata tttgtctttg ccgtttgagt tcacaatcga atcgagaagg atgagaataa
     2161 tgaatcgtaa ttttagacgg tgggcgaccg ctctgctctg cctctcgctc gcatcgcttg
     2221 cgctggcgtg cggtgcggcg tcgaataatt ccaacgcggg gacaaacgcg ccagccgagg
     2281 cgaacgtgaa aacgggagtc aagccggagc ctgacgcgga ggcggccatc atcgagacgg
     2341 actacgggcg catcgtgctt gagctttacc caaacgttgc gccgcagatg gtggcgcgtt
     2401 tcaaaaaatt agtgggtgag gggttctata acgggacggc gatccatcgc atcaacccgg
     2461 acttggggat catacagggc ggcgatccga actcgaaaga taacaaccct gcgaacgacg
     2521 gcatgggcaa ttccggcgag ccgaacgtcc cggcggaatt cagcgacatt ctgtacgagc
     2581 ggggaattgt cggcgcggcg cgcggcggcg gggatattaa ttccgccaac tgtcaattct
     2641 tcatcacgct gaaacgacaa gaggcgtttg agcagcccgg caatcgttac accgtcttcg
     2701 gacgcgtcat cgaaggtatc aacaacgccg acatcatcag caccgcgccc gtcacggacg
     2761 gcacggagca tccggcggat aagattctcg tcaagagcat cacgctcgcc ccgcgcgcca
     2821 acttcaaata gaacagtgac gggtgacaag ttaagaaagt gaatcgtgaa tcgtcaatcg
     2881 tgaatagaga ggacaggatt tcttctactc acgattgacg attcacgatt tacagccttt
     2941 ctcacttgtc acttgtcgtc cgtcacccgt cactaaattg agttgtcttc cgtcttcgag
     3001 cgcgggtcga gttcgaagcg gtcgcggtcg cggcggtcga tcttgtctat gaactcaagc
     3061 tcttcgttgt cttcgaagtt gacgccgacg gcgtgcgcgt tctcttccac gtcggattga
     3121 tcgggcgtcg ggttgtcgcc ggagacgacc tccgtgccgc tgtcgttgac atcttcccag
     3181 gaggcgtcga ggtcgccgcc cgcatcgcgc ggggaggcgg cggtgttctg ccgcagacgc
     3241 ccggccagtt gttccgggtc tttcggcgca cgcgcgatct cctcttccat gaactgctcg
     3301 atctccgggt cgggcacaaa gtccgggtcg ttgctcaagt actcaaagtc ttccggtcgt
     3361 ttcttcggca tctttccctc tccccgtgcg acaaatgaat cgaacacacg acgcgtcgcg
     3421 ctcatctccc tgccgacgct tcgtcgccgt gtttcacacg cactctattg gtttgcttct
     3481 ttgaacagcg acaatataac aaatgcccac ccgccccaca aacggcgtgg tcgaagccgc
     3541 gccgcgcgct ccaaaattga tgtgcttgac gttcgtgaga ggggcactta aacttaatcg
     3601 tattaaattt tcgccataaa tttgagcgtc cgcatgaatc gtcgcacact ctcggagcga
     3661 gcagaccgga ctgctttcgt taacagcctg acccgctgcc aactgcggaa ggaactttga
     3721 aattcccatg acccataatc tcttcaattc gctgcaacag ttcgacgccg gacaatttta
     3781 ttcgctgccg caactcgaac gagagggcgt cggccccgtc tccaaactgc ccgtcagcct
     3841 ccgcatcgtg ctcgaatcgc tcttgagaaa cttcgatggc aagaccgtcg aggaagagga
     3901 catccgcacg ctcgccaact ggcagcccaa cgccgagcgc acagacgaga ttccgtttat
     3961 cgtggcgcgc gtgctgttgc aggatttcac gggcgtgccg ctcttggttg acctcgccgc
     4021 gatgcgttcg gcggtcgcgc gcatgggacg cgacccgaag atcatcgagc cgctcgtgcc
     4081 cgtcgatctc gtcatcgacc actccgtgca ggttgacgcg tggagcacgc ccgatgcgct
     4141 ccagaagaac atggagacgg agtttaagcg caacaattcg cgctaccagt ttttgaagtg
     4201 gggtacgcag gcgttcgaga gttttcaggt ggtgccgccg ggaatcggca tcgtgcatca
     4261 ggtcaatctc gaatatctgg cgcaaggcgt catcgagcgc aatggagtct atctgcccga
     4321 cacgctcgtc ggcacggatt cgcacacgac gatgatcaac ggcctcggca tcgtggctta
     4381 cggcgtcggc ggcatcgaag cggaagcggg catgctcggc cagcccgtct actttttaac
     4441 gcccgacgtg gtgggcgtga atatgacggg cgcactgcgc gacggcgtga cggcgaccga
     4501 tctcgtcctg cgcgtcacgg agattctgcg cgcggcgaaa gtggtcggga agtttgtcga
     4561 gtttcacggc gaaggcgcgg cgagcatccc ggcttatgac cgtgcgacta tcgccaacat
     4621 ggctcccgaa tacggcgcga ctgtcggctt cttccccgtg gacgaaaaga cgctcgaata
     4681 ttttcgttcc accggcagaa gcgaggaacg cgtcaacgcc atacgcagtt attacgaagc
     4741 gcagggcatg ttcggcatcc cgcgcgcggg cgattgccag tacagccagt tgatcgaatt
     4801 gaacctcgca gagatcgagc cgagcgtgtc tggtccgaag cgtccgcagg atcgcatcga
     4861 actctccgac ctcaaagaac gcttcaacga actgttgcaa aagcccgtcg gcgagaacgg
     4921 ctacaacaag cagaaggaag aactcgacgc gcgtttcccc gtgaatctgg acgcgataac
     4981 caca
//
LOCUS       DASWNS010000183         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_33649, whole genome shotgun sequence.
ACCESSION   DASWNS010000183 DASWNS010000000
VERSION     DASWNS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33649"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..638)
                     /locus_tag="VGB76_06275"
     CDS             complement(252..638)
                     /locus_tag="VGB76_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0098542.1"
                     /translation="MRLFISKAAVLGLCACALLAACSPQDGSAPAAARSNNSAPAANA
                     NTTANTTAQASKQTPAQQAGETRRISIEETQAAIAGGSALVVDVRDENTYKTSHIKGA
                     KSIPLQQFEQRAGELPKDKLIITYCA"
     gene            complement(774..1283)
                     /locus_tag="VGB76_06280"
     CDS             complement(774..1283)
                     /locus_tag="VGB76_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098543.1"
                     /translation="MKRESTKTVIAALAGFYFLWCAYDPTRWHLIDGVNLLMHEAGHL
                     VFMPFGGLITVAGGSLFQVIVPAVFVAYFYRRGQIYSAALVLFWVGESLLNVSVYAGD
                     ALALELPLLGGGDSIHDWNYLLSSTGMLPSTRKIAAVLRLAGTLAIFAALFFSLRHAR
                     RDDAPAAEF"
     gene            complement(1331..2194)
                     /locus_tag="VGB76_06285"
     CDS             complement(1331..2194)
                     /locus_tag="VGB76_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0098544.1"
                     /translation="MKTPVLTAGALVAFAANSVLCRLALGRGAIDAASFSSIRLASGA
                     GMLLLITAAVERRPPAVHSGGKPASALFLFLYAAAFSYAYNGLGAGAGALILFGSVQA
                     TMIVFALRSGERPHVLEWAGLGLALCGLVYLVFPGLQSAPPLLSSALMSVAGVSWGLY
                     SLRGRSAASPLAETTRNFVLALPLAVLFNLLLLRDAHVSRAGVVLAVLSGALASGVGY
                     VVWYAALRGLTATRAATVQLAVPVLAAVGGVLFLSESISLRLLVAAIVILGGVGLALL
                     GRSQTANSKTD"
BASE COUNT          493 a          769 c          728 g          400 t
ORIGIN      
        1 acggcctccg cgtgaagtgg aggccgttga ctctcgccgc ttctgtatcc tctggccgtt
       61 cgcgccgctc tgaaccgcgc tccgtcattg ccgacagcta acggctaacg gctaaaggct
      121 tcttattgcg cactctccac gggaagtttc gcggcagtcc aggcgttcca accgccgagg
      181 agggcggctg cgttgtcgtg acctttgctt ttcagtatct gcaccgcacg ggcgctggtc
      241 tgttcgctcg gtcaagcgca gtaggtgatg atcaatttgt ccttcggcag ctcgcccgcg
      301 cgttgttcga actgctgcaa cgggatggat ttcgcaccct tgatgtggct cgttttgtac
      361 gtgttttcat cgcgcacgtc aacgacaagg gctgacccgc ccgcgatggc cgcctgcgtc
      421 tcttcgatgc tgatgcgacg cgtctctccg gcctgttgcg cgggagtctg tttcgaggct
      481 tgagccgtcg tgttggcggt cgtgttggcg tttgcggcgg gcgcgctgtt gttgctgcgg
      541 gcggcagcgg gcgcgctccc gtcctgcggg ctacaggccg ccagcagcgc gcaggcgcac
      601 aagccgagca cggctgcctt tgagataaat aaacgcataa cccttttgca ctcctcaaaa
      661 atatttctct ctccccgaag cgcggggcga gcgatgcccg gacagcatcg cataaatttc
      721 tcgcaaagtg aaagcgcggg gcatcagtgc gccgctcgtc gcctgtcgcc gcgttaaaac
      781 tccgctgcgg gcgcgtcgtc ccggcgtgca tgtctgagcg agaagaagag tgcggcgaag
      841 atcgcgagcg tgcctgcgag acggaggacg gcggcgattt tgcgcgtcga agggagcatt
      901 ccggttgaac tgagcaggta gttccaatcg tgaatcgaat ccccgccgcc gagcagcgga
      961 agctccaaag ccagcgcgtc gcccgcatac acggagacgt tcagcaaact ctcgcccacc
     1021 cagaagagga cgagcgcggc ggagtaaatc tgcccgcgcc ggtaaaagta tgcgacgaag
     1081 actgcgggca cgatgacctg aaagagcgag ccgcccgcga cggtgatgag tccgccgaac
     1141 ggcatgaaga cgagatgccc cgcttcgtgc atcaagaggt tgacgccgtc aatcaaatgc
     1201 cagcgcgtgg gatcataagc gcaccataaa aagtagaaac cggcgagcgc ggcgataaca
     1261 gtcttcgtcg actctctctt catacaggtt caactctcaa gtcttccgtc tctcttagtt
     1321 tcgccgcgcg ctaatccgtc ttcgagtttg ccgtttgcga gcgaccgagc aacgccagac
     1381 cgacgccgcc gagaatcacg attgccgcga cgagcagccg cagcgagata ctctccgaca
     1441 aaaagagcac accgcccacg gcagcgagta cgggcacggc caactgcacc gtcgccgcac
     1501 gcgtcgccgt tagtccacgc agcgctgcat accacacgac gtagcccacg cccgaagcga
     1561 gcgcgcccga caacacggcg aggacgacgc ccgcgcgtga gacgtgcgcg tcgcgcagca
     1621 acagtaggtt gaagaggacg gccaggggca acgcgagcac gaaatttctg gtcgtctcgg
     1681 caagcgggct ggctgcgctg cgtccacgca acgagtaaag cccccatgag acgcccgcaa
     1741 cggacatcag agccgaactc aaaagcggcg gcgcgctctg tagccccgga aacacgagat
     1801 agaccagacc gcagagcgcg aggccaagcc ccgcccactc caacacgtgc gggcgctcgc
     1861 cggagcgcag agcgaaaacg atcatcgtcg cctgtaccga gccgaataaa ataagcgcgc
     1921 ctgcgcccgc gccgagtccg ttgtaggcgt aagaaaaagc cgccgcgtaa agaaacagga
     1981 agagggctga cgcgggcttg ccgccgctat gcacggcggg cggccttctc tcaaccgccg
     2041 ccgtgataag caacagcatg cccgcgcccg acgccagccg gatgcttgag aaactcgccg
     2101 catcaatcgc cccgcgtccc aacgccagac ggcagagaac agagttcgca gcgaaggcca
     2161 cgagcgcacc tgcggtcaac acgggcgtct tcatacggcg cagagttttc cttccccgtt
     2221 ctgtgaagct agaagcgacg cggcaacaac cgccgcagcc gccgcgacga cacgagccgc
     2281 aaggcgttca ggttccggca gagtggttga gcttgacgtt cgcctccgcc acttgccgcc
     2341 cctgcctgtc cttactcaaa gtgacgagac aggcaactca aagttaaagc
//
LOCUS       DASWNS010000184         6400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_292988, whole genome shotgun sequence.
ACCESSION   DASWNS010000184 DASWNS010000000
VERSION     DASWNS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292988"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VGB76_06290"
     CDS             complement(<1..227)
                     /locus_tag="VGB76_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098545.1"
                     /translation="MLAGAVLQSAIGALIVPIFDQAFRRGGEVERTKTLFDLQHLIPE
                     SSFDAWRVIAILLITFTLAKGVAEYFSTYLMA"
     gene            619..1329
                     /locus_tag="VGB76_06295"
     CDS             619..1329
                     /locus_tag="VGB76_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0098546.1"
                     /translation="MNRKILIVDDDTSMRLLLAGYFRRLGYEVEEKESATEALQVALG
                     GSYDCFIFDVQMPGMSGLELLRKLRDRHIQTPALFLTAQDTLDDKVAGFEAGVDDYLT
                     KPFEPRELELRVEALLRRPAHASDAPEGEAQPQQRIEIGSLVIDKRRHEVTLEGALVE
                     LTPLEFQILERLASEPGRAWSRNDLLDEVWSTEYEGYQRNVDPHINRLRQKIELDPKN
                     PRYVLTVRGVGYKLNDAL"
     gene            1353..2906
                     /locus_tag="VGB76_06300"
     CDS             1353..2906
                     /locus_tag="VGB76_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0098547.1"
                     /translation="MLKTLRLLVLAALALPAFVHAQPETSAARKPAEGASQIAAPLKL
                     RWPARPRARRYRLQVALDEKFNDIVFDRAVEGLEQEVSGLQPGKYYWRVAPATPETGR
                     YSAPALVEAALPVPVAPTFTVLRPPTDVGWQAAIGTVARPFAARLRGGKNFDLVALNS
                     DGTVYALDAADGTPLWVARSRATAPAADAKDKPRTSHFTPLVLPATAESRSIVAAGFG
                     EGIRGLDGETGRELWRSVLQGRPAGGALAALGGSGEGATSDFVVATSDPDTLYVLEGC
                     TGRIVSKTKLGAPLIGSPIPFSHGEINGIAYSLENRQLEIRKRDGSLVRSVKFDVPFS
                     TPPLVLATPRGTLVIIGTDHGLLFFEGSELKPLGRITTEKDAPRGRLTAADLNEDGTI
                     EVVMVTKNGRVAVISTVGKINWASEGATDAYMATFAHLNTDGVLDVVVASGSSFALGF
                     SGRDGTLIWRADENAKPESVASEERMLRTLTGAISSTGVPLLVGGDATRSAVRAVGLP
                     SIANKAAVK"
     gene            2926..3090
                     /locus_tag="VGB76_06305"
     CDS             2926..3090
                     /locus_tag="VGB76_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098548.1"
                     /translation="MNGDLMIWGIALTLFAVWLIGVLLGKGGFVHVLLLCAISVAVVQ
                     WMSERRARRG"
     gene            3299..4894
                     /locus_tag="VGB76_06310"
     CDS             3299..4894
                     /locus_tag="VGB76_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0098549.1"
                     /translation="MKYSRTQEKLLRGVFVSLLLLASATVSPHVSAQSDMRRETVAIT
                     YPLDQNVSVRFRGTTRLPRLTGEAKVRRSGRRGTRVELSLKNLPRAIEVGGVYSTFVL
                     WAITPEGRADNLGEIKRSGSFIVNSKLDVTTPLQTFALIVTAEPHFLVRSPSRAVVLE
                     NLPPNNPGDATVDTVSVRYIGNTSDYLSNSRVPEIADADYVRTPTSLLGARQAINLAR
                     FAGAERDATAELKEATDQIEIAENAWRLKQPDEDVDAAARRATSLAMRAEEVAESRKA
                     ARQRREEIARRDDAVRDAERSADSANQEIANLRAALQREERARELSDRDLSNANQQVR
                     ELRTEVARLRDEMQAMRSESEDAKLKLARIEGERSAEVQRQEAQRRLEQQQQAAATLR
                     QSLARYGTVRDTGRGITLVLPDNLWSGARAADLAPASSATVEPLAALFANYPDFQILI
                     EVYSDSRGDMAALRQLTQARADALAGRLVTAGVENGRVQATGMGTDNPVASNSTPAGR
                     TRNRRTEITLVPINASSASANER"
     gene            4993..>6400
                     /locus_tag="VGB76_06315"
     CDS             4993..>6400
                     /locus_tag="VGB76_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098550.1"
                     /translation="MSLLEVLQLVGYSMGAVLTLWMSVLLARQRRAVERAERVLLALA
                     VGIGLWHASNLFLTLRALLGLDPQSWSGVLRVADTLAVTSITLAYSLLLHVHLHLWAR
                     AQHRGFTRFERARVYMSYIPAIFLAVAIPRIWSGAYAPMLEKLNFFVLPFGVWATYVL
                     ALIAGTDWLLARASDSRTERRLMQTLAASFVGIALLLLAAYALGVGRGTVAGHYLQTF
                     ANLGSLLPTALIAYHIYRYRYLELIIKESLVVASFAAVVLVVYLYGIRTLALALTARY
                     NLRASAVESLLVLAFALVVAPLRAWLDKRFHKLFEREASIYRDVLARIGAQAGGYKQL
                     PELLRFIEERTSSELGLRRVRFYVRPAYNLAPSEPDAYSEANAGETTNEDGEWEERVL
                     ELASENEGDALENVKLLREHGFEMASHLKRENKSLGLMLVDAAPDALTTDVRAVLEVL
                     AGQVALTLEDYRLIEENVR"
BASE COUNT         1208 a         2054 c         1982 g         1156 t
ORIGIN      
        1 gccatcaggt aggtggaaaa atattcggcg acgcctttgg cgagcgtgaa ggtgatgagc
       61 aggatggcga tgacgcgcca ggcgtcgaaa ctggattcgg ggatcaggtg ttgtaaatca
      121 aaaagcgtct tggtacgctc cacctcgccg ccgcgccgga aagcctgatc gaagatcggc
      181 acgatgagcg cgccgattgc gctctgcaag acggctcccg cgagcatcgc aaaagtggca
      241 agcgtaaact ttccccagtg aggcttgaga tatgttagta gtcgccggag atcgtgcatc
      301 gtttcgggag cgcgcgcgtg tctgcgattc gtttcgttcg agcggggaag gataattaaa
      361 gttcaactgt aacggaacaa aaactatacg tacacttcaa cgcaagcgtc aaggcggaag
      421 acaaaagggg caacgcgcga ggggctgcgc gccggacgca ccgctccggc gcgactcccg
      481 cacgccgccg cgcgcgactt ttttctgtaa ccttgccttt tttggcttat aatcgccgcg
      541 tcttaaaatt aacgaatctg aacaaggtaa ggcgggacga ctttcccgcc taaattacaa
      601 caggtgaatc gagaaccagt gaaccgaaaa atcctgatcg tcgatgacga tacgagcatg
      661 cgcctgcttc tggcgggcta cttccggcgg ctcggctacg aagtggaaga gaaggagagc
      721 gcgaccgaag ccctacaggt cgcgctgggc ggcagctacg actgttttat cttcgacgtg
      781 cagatgcccg gcatgtccgg cctcgaactc ttacgcaaac tgcgcgaccg gcacattcaa
      841 acgcccgcgc tctttttaac cgcacaggac acgctcgatg acaaggtggc cggtttcgaa
      901 gcgggcgtag acgattacct gaccaagccc tttgagccgc gcgaactcga actgcgcgtc
      961 gaagccctgc tgcgtcgccc cgcgcacgcg agcgatgcgc cggaaggtga ggcgcagccg
     1021 caacagcgca tcgagatcgg aagtctcgtc atagacaaac gccgtcatga ggtgacgctc
     1081 gaaggcgcgc ttgtcgaact cacgccgctg gagtttcaga ttctcgaacg cctcgccagc
     1141 gagccggggc gcgcgtggtc gcgcaacgac ctgctcgacg aggtgtggag cacggagtac
     1201 gaaggttatc aacgcaacgt cgatccgcat atcaatcgcc tgcggcagaa gattgaactc
     1261 gacccgaaga acccgcgcta cgtgctgacg gtgcgcggcg tcgggtacaa actcaacgac
     1321 gccctatgat gctaccaaga cgccccttgc ccttgctcaa aactctccgc cttctcgtac
     1381 tcgctgcgct cgcgctcccg gccttcgtcc acgcgcaacc cgaaacctcc gccgcacgca
     1441 aacctgccga gggcgcgtcg cagattgccg caccgcttaa actccgttgg cctgcgaggc
     1501 cgcgtgcgcg tcgctaccgg ctgcaagtgg cgctcgatga aaaattcaac gacatcgttt
     1561 tcgaccgcgc cgtagaggga ctcgaacagg aggtgagcgg cctacagccc ggcaaatact
     1621 actggcgcgt cgcacccgcc acgccggaga ctggccgtta ttccgctcct gcgcttgtcg
     1681 aagccgcgtt gcccgtcccc gtcgcgccga ccttcacggt gctgcgcccg cccacggatg
     1741 tcggttggca ggcggccatc ggcacggtgg ctcgtccctt cgccgcgcgc ctgcgcgggg
     1801 gtaagaattt cgatctcgtc gcgttgaact cagacggcac ggtctacgcg ctcgacgccg
     1861 cagacggaac tcctctgtgg gtcgcgcgtt cgcgtgcgac tgcgcccgcc gccgacgcga
     1921 aagataagcc gcgcacgagt cacttcacgc cgctcgttct gcccgcaacg gcggagagca
     1981 ggtccatcgt cgccgccggg tttggcgaag gcatacgcgg tctggacggg gagacggggc
     2041 gcgaattgtg gcgcagcgtg ttgcaggggc gtccggcggg cggcgcgctc gcagcgttgg
     2101 ggggcagcgg cgagggcgcg acctccgact tcgtggtagc gacgagcgac cccgacacgc
     2161 tctacgtttt ggaagggtgc acggggcgca tcgtctcgaa gacgaaactc ggcgcaccct
     2221 tgattggctc tccgattcct ttctcgcatg gcgagattaa cggcatcgcc tactcgctcg
     2281 aaaacaggca gttggagatt cgtaagcgcg acggctcgct cgtgcgttcc gtcaagttcg
     2341 acgtgccttt ttcgacgccg ccgctcgttc tggcaactcc gcgcggaacg ctcgtcatca
     2401 tcggcacgga tcatggcctg ctcttctttg aaggctccga attgaaaccg ctcggtcgca
     2461 tcacgacgga aaaggatgcg ccgcgcgggc gactgaccgc cgcagacctc aacgaagacg
     2521 gcaccatcga ggtcgtgatg gtgacgaaaa acgggcgcgt cgccgtcatc agcacggtgg
     2581 gcaagatcaa ctgggcttcg gagggcgcga ccgacgctta catggcgacg ttcgcgcact
     2641 tgaacacgga cggcgtgctc gacgtagtgg tcgcctccgg ctcctctttc gcactcggct
     2701 tctccggtcg cgacggcact ttgatctggc gcgccgatga gaacgcgaag cctgagagcg
     2761 tggcctccga agagagaatg ttacgcacgc tgacgggcgc aatctcaagt acgggcgtgc
     2821 cgctcctcgt cggcggcgac gccacgcgtt ccgccgtgcg cgccgtcggc ttgccgtcga
     2881 ttgcgaacaa ggcggcggtc aaataaattc agcgcgcatg gcgacgtgaa tggtgacttg
     2941 atgatctggg gcatcgcact gactctgttt gcggtctggt taatcggcgt cttgttggga
     3001 aaaggcggct tcgtacacgt cctgctgctg tgcgccatct cggtcgccgt cgtgcaatgg
     3061 atgtccgagc ggcgcgcgag gcgcggttga gggcatggcg gcaagtgcga aacggtaatg
     3121 cgaaaggttg cggaagtttt aagaggctgc tttgaagttt gggcagcctc ttcccgcacg
     3181 ggcgcggcaa ctctctcacg cgacccgcgc atccattcag acgacatctt gggttctccc
     3241 gtgcctgcgc ctttgaccgc gcgcacatct tttcaaaaat cttgagaggc taagctatat
     3301 gaagtattcg cgcacacagg aaaaactgct ccggggcgtg tttgtctcgc ttctgctgct
     3361 cgcgtcggcc accgtctccc cgcacgtctc ggcgcaaagc gacatgcggc gtgagacggt
     3421 tgccatcacc tatccgctcg accagaacgt ctccgtgcgt tttcgcggca cgacccgcct
     3481 gccgcgcctg acgggcgagg ccaaggtccg ccgcagcggg cgacgcggga cgcgcgtcga
     3541 actcagtctc aaaaatctcc cccgcgccat cgaagtcggc ggcgtctatt ccactttcgt
     3601 gctctgggcg atcacgcccg aaggccgcgc cgacaatctc ggcgagatca aacgcagcgg
     3661 cagtttcatc gtcaactcga aactcgacgt gacgacgccc ttgcaaacct tcgccctgat
     3721 cgttaccgcc gaaccgcact ttctcgtgcg tagtccgagc cgcgcggtcg tgctcgaaaa
     3781 cctgccgccg aataatcccg gcgatgccac ggttgacacc gtcagcgtcc gttatatcgg
     3841 caacaccagc gactacctga gcaacagccg cgtgccggag atcgccgacg ccgattacgt
     3901 ccgcacgccg acctcgctcc tcggcgcgcg tcaggccatc aacctcgcgc gcttcgccgg
     3961 ggcggagcgc gatgcgaccg ccgagttgaa agaggcgaca gaccagatcg agatcgcgga
     4021 gaacgcgtgg cggctgaagc agccggacga ggatgtggat gcggcggcgc gtcgtgcgac
     4081 gagtctggcg atgcgcgccg aagaggtggc ggagtcgcgg aaggctgcgc ggcaacgtcg
     4141 tgaagagatc gcgcggcgcg acgatgccgt gcgcgatgcg gaacgttcgg cggattcggc
     4201 caatcaggag atcgccaacc tgcgcgccgc cctgcaacgc gaagaacgcg cgcgcgaact
     4261 ctccgaccgc gacctctcaa acgctaacca gcaggtgcgc gaactgcgca cggaagtggc
     4321 gcggctgcgc gacgagatgc aggcgatgcg cagcgaatcg gaagacgcga agttgaaact
     4381 cgcgcgcatc gaaggcgagc gcagcgcgga ggtgcaacga caggaagcac agcgtcgcct
     4441 cgaacagcaa cagcaggccg ccgccacgct gcggcagtct ctggcgcgct acggcaccgt
     4501 gcgcgacacg gggcgcggca tcacgctcgt cctgcccgac aatctctggt cgggcgcacg
     4561 cgccgccgac ctcgcgcccg cttcctccgc cacggtcgaa ccgctcgccg cgctcttcgc
     4621 caactatcct gactttcaaa tcctgatcga agtttattcc gacagtcgcg gcgacatggc
     4681 tgccttgcgc cagttgacgc aggcgcgcgc cgatgcgctc gccgggcgtc tcgtcaccgc
     4741 cggagtggag aacgggcgcg tgcaggcgac gggcatggga accgataacc ccgtcgcttc
     4801 caacagcacg cccgccggac gcacacgcaa tcgtcgcacg gagatcacgc tcgtcccgat
     4861 caacgccagc agcgcgtcgg ccaacgaacg ctgacaacat aagaaacgta aatcgtgaat
     4921 cgtaaatcgt gaatagatca agaaaggctt tcctctattc acgatttacg attcacgatt
     4981 tactatgtgt ccatgtcgct gcttgaagtt ttacagcttg tcggctacag catgggcgca
     5041 gtcctgaccc tgtggatgag cgtgctgctg gcaagacaga ggcgcgccgt cgagcgtgcg
     5101 gaacgcgtgc tgctggcgct cgccgtcggc atcggcctct ggcacgcgag caacctgttt
     5161 ctcacgctgc gcgccctgct cggcctcgac ccgcaatcgt ggtcgggcgt gctgcgcgtg
     5221 gccgacacgc tcgccgtcac gagcatcacg ctcgcctact cactgctcct acacgtccac
     5281 ctgcatctct gggcacgcgc gcagcatcgc ggcttcacgc gcttcgagcg ggcgcgcgtc
     5341 tacatgtctt acatcccggc gatcttttta gccgtcgcca tcccgcgcat ctggtcggga
     5401 gcgtatgcgc cgatgttgga gaagctgaac ttcttcgtcc tgcccttcgg cgtgtgggcg
     5461 acttatgtgc tggctctcat cgccggaacc gactggctgc tcgcacgcgc ctccgactcg
     5521 cgcaccgagc gacgcctgat gcaaacgctc gccgcctcgt tcgtcgggat cgcgctactg
     5581 ttgctcgccg cttacgcgct cggcgtcggg cgcggcaccg tcgccggaca ttatttgcag
     5641 accttcgcca acctcggctc gctcctgccg accgcgctca tcgcctatca catctatcgt
     5701 tatcgttatc ttgaactgat catcaaagag agcctcgtcg tcgcatcgtt cgcggcggtc
     5761 gtcctcgtcg tctatctcta cggcatacgc acgctcgcgc tcgcgctgac ggcgcgctac
     5821 aacctgcgcg cgagcgcggt tgagtctttg ctcgtgcttg ccttcgcgct cgtcgtcgcg
     5881 cccctgcgcg cgtggctcga caagcgtttc cacaaactgt tcgaacgcga agcctctatc
     5941 tatcgagacg tgctggcgcg catcggcgcg caggccggag gttataagca gttgccggaa
     6001 ctgctccgct tcatcgaaga aagaacctca agcgaacttg gcctgcgccg cgtacgcttc
     6061 tacgtgcggc cagcctacaa cctcgcgccc tccgaacccg acgcttactc cgaagcgaac
     6121 gcgggcgaga cgacgaacga ggacggcgaa tgggaagagc gcgtgttgga actggcgtcg
     6181 gagaacgaag gcgacgcgct ggaaaatgtg aaacttctgc gcgagcacgg cttcgagatg
     6241 gcctctcact tgaagcgcga aaacaaatcg ctcggcttga tgctcgtaga tgccgcgccc
     6301 gatgcgttga cgacggacgt gcgtgcagtc ctggaagtgc tggcggggca ggtcgcgctc
     6361 acactcgaag actatcgttt gatcgaagag aacgtgcggc
//
LOCUS       DASWNS010000185         5285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_423369, whole genome shotgun sequence.
ACCESSION   DASWNS010000185 DASWNS010000000
VERSION     DASWNS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423369"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            822..1931
                     /locus_tag="VGB76_06320"
     CDS             822..1931
                     /locus_tag="VGB76_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098551.1"
                     /translation="MNKLTEEQRRRISRRRGERGFSLLQMVIVLSIIAVISTVALVSV
                     PAARAGQRRVNSARMFAAYLEKARVDSVRRRPEDAAQSSSITILNANTYSVFMDFDGT
                     GVPTTRTFTLDSGIVFINSDIGAVVSFDWRGRTGNDRQFLIVNGDRPNNNPEDTNYVT
                     PVAVSGFGDVTINDEVYTPAINSNTSGFASPTPAPSTTPTPEGTPSGTPSATPSPNTT
                     PTPDGTPNPTPSATPSATPSATPNATPSATPSATPSATPSATPNPTPSSTPAPPACSL
                     QVTPAAVSVVKQGKSTATVTVRVPSATTATVVTASSASGTNVSVSPAVASVPSGGSAS
                     FTIKSLKNNAGFVYQVKFTAPNCGESIVPVTVTNN"
     gene            1944..2579
                     /locus_tag="VGB76_06325"
     CDS             1944..2579
                     /locus_tag="VGB76_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0098552.1"
                     /translation="MKFSSKNAQGFTLIETCIALVVMMVVGLGATSLFLFALRNNTGG
                     SERSQALAIAQQRLEELRTIKYDDPQLAIGQTISTVVLQDVAAPGGVAAAAAAPSSGG
                     GAQAYSSAASGGSLISPSGKPGKSSPTPTPTPGGGGGGGGGGGGGVGIGGYKLVLDVE
                     GLPAGSASPTQKRITVTVTPENGYGAAAWMNQNPVVIVIHRSSPSSGPYRL"
     gene            2629..3303
                     /locus_tag="VGB76_06330"
     CDS             2629..3303
                     /locus_tag="VGB76_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0098553.1"
                     /translation="MRQTTQTTDREAGFSLMELIVAMCVMMIITGAATSLLVGAFNVR
                     SREDQRTEAIGDARRALNILSRELANSGYQLPRGLTYTSAAGASPVPMNGLIPSDCDD
                     ESITFVANLNASLGTGDEDVNDDSEALKFQFFQNGDNNFLVRTDLRPGGDSIVLANRI
                     DEVDFEYLNDANVPVAVTAAVRIRITMVVTLNPVGQPGTTSYHPPAQERLSSIVDLRN
                     ATLGTY"
     gene            3355..>5285
                     /locus_tag="VGB76_06335"
     CDS             3355..>5285
                     /locus_tag="VGB76_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098554.1"
                     /translation="MKKNTKKRNSTSTNRCRRNERGAALIMMLLISMLLLAAGGALIM
                     TTALSTTTVYEATPEMQAYYAAETGLEDVLTVLRGNVAPQSAGMAAPAASKGSSVLAS
                     VSSFASSAYSAFMPTAHAAAPTGGNTTDEIPDAKKINMRRALDHAYADHPNDPENAPD
                     LRLSRWLDYDYTPPNGDYPDRVTLNDNYSPLTGTAYRVTLSDADDSTSVAYTTTGLFK
                     AGGPGVVISGNGRSITFNNILGPAVGTGTVTYVPRTDQTAFDSYPAIASNLGSFNISS
                     TGIGASIPPGVTFELFVNQKAPWQGSASLNANLSGLVNPVAVLNSLQVGFPKDTLLVD
                     GTTIKITNLVGGLLSLLVPPAGGSVPLQANVTAPEPKRVLVRSTGIGPRGAQKILEML
                     LTRANLEFEAPATLTVRGSSDCSGMTFSTGSSNAKTYTGEDLNGKEPQRPAFAVTACD
                     YDDAVNGTTKGGTVEGPPQIGILDNGMAAGTMTTAPVQMPEFLKTPDKARQYLNELEA
                     TARAQGRYINPASGTSTTVNSGTLDNPVFTFVDGDCELTGGAGLLVVTGNVNMSGNTS
                     FQGVVLVMGEGSVNRNGGGSGDIDGSMVISKFERTWPASEDDPSIPNNVEYPFLAPSF
                     NTNGGGASTMQYSSTAVARA"
BASE COUNT         1054 a         1739 c         1546 g          946 t
ORIGIN      
        1 aggccgacgc gtcggagttt gaaccaaaga caaactgagg gatgaaggat gagggatgag
       61 ggatgaggga tgaaggatga gggatgaata agaaggagca ttcaactctc ttgagtgctc
      121 cttacttatt catccctcat ccctcatccc taaaattttg cgccccggcg gcctgagagt
      181 cgcacggggc gctttttcgt ccgactcgcg catggcgtgc ggcaccgcgc gcaagcggac
      241 gatccttttg tttctcccgc ttgattcggg ctgcgaaagt cggagtcgca cgcgcgccgc
      301 accttcccct tcgcagttgt tccctccttg ttcggtcgct ccttaccggc ctgttccaaa
      361 cgctgcccct ctaactttgc agaaccgcgt ccgtcgccgc tttcgtgcga cccgcacgcc
      421 ttattgcaat tgtcaggcca ccttgcgcgc ccgccccgac ttctcgcttc aaacaacggg
      481 cacataaacc tttgccgaca ttcgcgcacc ctgatgtttc gacccgccac gcctttgccc
      541 ggcgcacctg atgtttccgt tggtgacaag gtgtgtaacg cgggttcgtc ttttgccgcg
      601 cggctgccgg atgtccggag agttgtgtag gccgcttcct gttctgttac ttaaggtgac
      661 gcgcggcgcg cgcggcgaca taccgggcag gcgaaatcaa cgggaaatag agccattatc
      721 cgagtggcac cgcccttgcc aagaagaggc gggacgcaca cgtccgtcga gcctgtcagt
      781 tgaaaatgtg aagcgggctt tcctgtggag gctatagagt gatgaacaag ttgaccgagg
      841 aacagaggcg aagaatttcg agaaggcgcg gcgagcgcgg cttctcgctc cttcagatgg
      901 tgatcgtgct ctcgatcatc gccgtcatct cgacggttgc gctggtgagc gtcccggcgg
      961 cgcgcgccgg acaaagacgc gtcaactcgg cgcgcatgtt cgcagcctat ctggagaaag
     1021 ctcgcgtcga ttccgtccgc cgccgccccg aggatgccgc acagagttcg tcaatcacta
     1081 tcctcaacgc caacacctac agcgtcttta tggacttcga cggcacgggc gtccctacca
     1141 cgcgcacctt cacgctcgat tcgggcattg tttttatcaa ttccgacatc ggcgcggtcg
     1201 tctctttcga ctggcgcggg cgcaccggca atgatcgcca gttcctgatc gtcaacggcg
     1261 accgccccaa caacaacccg gaagacacta actacgtgac tccggtggct gtctccggct
     1321 tcggcgacgt gaccatcaac gacgaagttt atacgcccgc catcaactcc aacacctccg
     1381 gtttcgcgtc gcccacgcct gcaccctcaa ccacgcctac gcctgagggc actccgagcg
     1441 gtacgccgag cgcgacgccc tcgcccaaca caaccccgac accggacggg acgccgaacc
     1501 cgactccgag cgcaacgccg agtgcgacgc cgagcgcgac tccgaacgca acgccgagcg
     1561 cgactcccag cgcgacgccg agcgcaacgc cgagcgcgac gccgaacccg acgccgagtt
     1621 caacaccggc tccaccggcc tgttcgctgc aagtcactcc ggctgcggtg tcggtcgtca
     1681 aacaaggcaa gagcacggcc accgtcacag tgcgcgtgcc gagcgcgact accgctacgg
     1741 tggtcacggc gagttcggcc agcggcacca acgtatcggt ttcgcctgcc gtcgccagtg
     1801 tcccgtccgg cggctccgcc tcgttcacga tcaagtcgct gaagaacaac gcgggattcg
     1861 tctatcaggt gaagttcact gcgcccaatt gcggtgaaag catcgttccg gtcacggtga
     1921 cgaacaacta ggagaagcgc aagatgaaat tcagttcaaa aaacgcacag ggcttcaccc
     1981 tgatcgaaac gtgcatcgct ctggttgtga tgatggtggt cgggctgggc gcaacttcgc
     2041 tcttcctctt cgcgctccgc aacaacacgg gcggcagcga gcgttcgcag gctttagcca
     2101 tcgcgcaaca gcgactcgaa gaattgcgca ccataaaata cgacgatcca cagctcgcca
     2161 tcgggcagac aatttcgacc gtcgtgctcc aagacgtggc cgcgcccggc ggcgtggcgg
     2221 ctgcggctgc cgcgccgagt tcaggcgggg gcgcgcaggc gtatagctct gcggcgtccg
     2281 gcggcagtct catctcgccc tccggtaaac ccggtaagag cagccccacc ccgacgccga
     2341 cccccggcgg tggcggcggt ggcggtggtg gcggtggcgg cggtgtcggt atcggtggtt
     2401 ataagctcgt cctcgacgta gagggcttac ccgccggttc ggccagcccg acgcagaaac
     2461 ggatcacggt cacggtgacg cccgaaaacg gctacggggc agcggcgtgg atgaatcaga
     2521 acccggtggt cattgtcatt catcgcagca gcccttcctc tggaccttac aggctgtagt
     2581 tcagggacga gtacgaacag gccggatgat tcggggagac gaaagttcat gcgacaaact
     2641 acacagacga cagatcgaga ggcgggcttt tcgctgatgg agttgatcgt ggcgatgtgc
     2701 gtgatgatga tcatcacggg cgcggccacg tctctgctcg tcggcgcgtt caacgtgcgc
     2761 tcgcgcgaag accagcgcac cgaggccatc ggcgacgcgc gccgcgcgct caacatcctc
     2821 tcgcgcgaac tcgccaactc cggctaccag ttgccgcgcg ggctgaccta cacgtcggct
     2881 gccggtgcgt ctcccgtccc gatgaacggc ctcatcccga gcgactgcga cgacgaatcc
     2941 atcactttcg tcgccaacct caatgcgagt ctggggacgg gtgacgagga tgtcaacgac
     3001 gacagcgagg cgttgaaatt ccagttcttc cagaacggcg acaacaactt tctggtgcgc
     3061 accgatctcc gtccgggggg tgactccatc gtgctcgcca accgtattga cgaggtggat
     3121 ttcgaatatc tgaatgatgc gaacgtgccc gtcgccgtca cggcagcggt gcgcattcgc
     3181 atcacgatgg tcgtcacgct caatccggtc ggccagcccg gcacgaccag ctaccatccg
     3241 cctgcgcagg aacgcctctc gtctatcgtt gatctacgca atgccacgct tggcacatac
     3301 taaatgaagt gagcaggggc aaatgaatgc gccccgcaga gtgaggttga caggatgaag
     3361 aagaacacaa agaagcgaaa ctctacctcg accaatcgct gccgtcgcaa cgaacgcggc
     3421 gcggcgctca tcatgatgct gctcatctcg atgctgctgc tggcggcggg cggcgcactg
     3481 atcatgacga cggcgctctc caccacgacc gtctacgagg ccacgccgga gatgcaggca
     3541 tactacgccg ccgaaaccgg actcgaagac gtgctgacgg tcttgcgcgg caacgtcgcc
     3601 ccgcagagcg cagggatggc tgcgcccgcc gcttcaaagg ggtcgtccgt cctggcctcc
     3661 gtctcctctt tcgcctcctc ggcttactcg gccttcatgc ctacggcgca cgccgctgcg
     3721 cccacgggcg gcaacacgac cgacgaaatc cccgacgcga aaaaaataaa catgcgccgc
     3781 gctctcgatc acgcctatgc cgatcacccg aatgatccgg agaatgcgcc cgacctacgc
     3841 ctctcgcgct ggttggacta cgactacacg ccgcccaacg gcgactatcc ggatcgcgtg
     3901 acgctcaacg acaactacag cccgctcacg ggtacggctt accgcgtcac gctcagcgac
     3961 gcggacgaca gcacttccgt cgcctacacg acgaccggcc tcttcaaagc cggtggaccc
     4021 ggagttgtca tcagcgggaa cggtcggtcg atcaccttca acaacatact cggcccggca
     4081 gttggcaccg gcacggtcac ttacgtaccc cgcaccgatc aaaccgcttt cgacagctat
     4141 cccgctatcg cgagcaacct cggcagtttt aacatctctt ccacaggcat cggggcgagc
     4201 atcccgcccg gcgtcacctt cgaactgttc gtcaatcaaa aggctccttg gcagggcagc
     4261 gcatcgctca acgccaatct cagcggtctg gtcaaccccg tcgccgtctt gaactctttg
     4321 caggtcggtt tcccgaaaga cacgctcctc gtggacggca cgacgatcaa gatcacgaat
     4381 ttggtcggcg gccttctgag tctgctcgtg ccgcccgccg gcgggtctgt gcccttgcag
     4441 gccaacgtca ccgctcccga accgaagcgc gtgctcgtgc gttcaaccgg catcgggccg
     4501 cgtggcgcgc agaagattct ggaaatgctc ctcacccgcg ccaacctcga attcgaagct
     4561 cccgcgacgc tcaccgtgcg cggatcgagc gactgtagcg gcatgacttt ctccaccgga
     4621 agcagcaacg ccaagactta cacgggcgaa gacttgaatg gcaaagaacc gcagcgtccg
     4681 gccttcgccg tgacggcctg cgactacgac gacgcggtga acggaacgac caaaggcgga
     4741 accgttgaag ggcctccgca aatcggcatt ctcgacaacg gaatggcggc gggtacgatg
     4801 acgaccgccc ccgtgcagat gccggaattt ttgaagacgc cggataaagc tcgccagtac
     4861 ttgaacgagt tggaagcgac ggcgcgcgcg caaggccgct acatcaaccc ggccagcggg
     4921 acttccacaa ccgtcaatag cggcacgctg gacaatccgg tcttcacctt cgtagacggc
     4981 gattgcgaac tgaccggagg cgcagggctg ctcgtcgtga cgggtaatgt gaacatgagc
     5041 ggcaacacct cgtttcaggg tgtggtgctg gtgatgggcg aaggctctgt gaaccgcaac
     5101 ggcggcggca gcggcgacat cgacggctcg atggtcatct ctaaattcga gcgcacgtgg
     5161 cctgcctccg aggacgatcc gtcgattcct aacaacgtgg aatatccgtt cctcgcgccg
     5221 tcctttaata ccaacggcgg cggcgcatcc acgatgcagt acagttcgac cgccgtggct
     5281 cgcgc
//
LOCUS       DASWNS010000186         7871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_241368, whole genome shotgun sequence.
ACCESSION   DASWNS010000186 DASWNS010000000
VERSION     DASWNS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241368"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..534)
                     /locus_tag="VGB76_06340"
     CDS             complement(121..534)
                     /locus_tag="VGB76_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY0098555.1"
                     /translation="MSPLFDWLKTLFAARREAARVGGESSATGGAASDVEEGEIDLDN
                     PFPEPVRLEITDVFDLHTIAPRDVERVVQEYLTEAHRAGFLSVRIIHGKGQGVQRRLV
                     QSILARTPFVREWTDAPPQSGGWGATVAHLKIEKY"
     gene            737..1672
                     /gene="mdh"
                     /locus_tag="VGB76_06345"
     CDS             737..1672
                     /gene="mdh"
                     /locus_tag="VGB76_06345"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY0098556.1"
                     /translation="MGKRNKITVVGAGNVGATAAHWLASKELGDVVLVDIIEGMPQGK
                     ALDLAQAAPVEGFDARMIGSTNDYSLTKDSDVVIITSGLARKPGMSRDDLLKTNAGIV
                     SSVVDEVVKYSPDSILIIVSNPLDAMCQVALRRSGFPKHRVIGMAGVLDSARMRCFLA
                     EALNVSVENVTAFVLGGHGDTMVPLPRYSTCAGIPVTELLSQEQIESIVKRTANGGAE
                     IVGLLKTGSAYYAPSSASVEMTEAILKDKKKILPCAAYLEGEYGINGLYVGVPCKLGA
                     NGLEQIIEINLTAEERIALQKSAASVQELVDVLGL"
     gene            1826..4540
                     /locus_tag="VGB76_06350"
     CDS             1826..4540
                     /locus_tag="VGB76_06350"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HEY0098557.1"
                     /translation="MSQKNAPAAVAKFAFRKSGLELERAARAGAFYSVTGRRAAAFGY
                     EHRPLEAWVYPLKILDDFALSFNIEGYPLEFGGADTLARINVRPEATTFTYSHAAFTV
                     RQTIFAPLDEPGIVMLLDVQSALPLTVNVSFRPRLRLSWPAGLMTGSLSWNEKSRVYY
                     ITEETRRFTGIVGSPAATDVSVMPYQEEPRGVPARFRIPVPAGEMSSGFIPIIIAGSV
                     EGREKAKAAYDRLLSAIPSLYEKNVAHYERLEAETLGISTPDRELDEAFAWAKVGVDK
                     GVVTNPLLGTGLVAGYRTSGESERPGFAWFFGRDALWTAFALNSYGDFQTTRTALEFL
                     KKFQRADGKIPHEISQSASLIPWFTDYEYPWASADATPLYVIAHADYFRATGDLEFLR
                     ANWDSILKAYRFTEATDTDANGLVENTKFGHGWVEGGGLYPAHEEIYMQGLWVAASRD
                     LAGMAAALKEGKVAALARANAERTRAATEETYWLPQRGFYAFATKRPATEPPEADPGP
                     NRAQRQARMNALKDARLYDEDTVLPAVPLWFKTLTDERAELQLDALGAGALATDWGAR
                     ILSNQSQLYDPLSYHNGSVWPLFTGWASLGAYRYGRPHVGFQALMANALLTRQGAEGS
                     VTELLSGDFNAPFGRSSHHQVWSEAMVVTPLVRGLLGLEPDAADDDVLRLALQLPVNW
                     DRLEVRNLRLARGYYDMRLVRRTGQTRLSFIPHRASQLANAKGSAEGLRKIAVSISLP
                     LDARMCRVTKPRLAGSSLEQQPTAPVDCDARTYKLTRMGDVQRVEAMFVPEASEVGLV
                     FSYEEGTDVYVAPETPVAGARNRGLRILRSRADASHLRLLVEGLGGQTYTLGTRSSRA
                     LLATSGVEIKKGTDAGSQLLITFAGTPDRYVRREIDIPLQASKPGRRN"
     gene            complement(4633..5457)
                     /gene="sdhB"
                     /locus_tag="VGB76_06355"
     CDS             complement(4633..5457)
                     /gene="sdhB"
                     /locus_tag="VGB76_06355"
                     /inference="COORDINATES: protein motif:HMM:NF006391.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY0098558.1"
                     /translation="MSAHTNQTNSTEATADRSYTPAPEQQRAQKFVEIRIKRRDRPDA
                     PQRWEEFRVPYRPNLNIISCLMDIQKNPVTKDGTKTTPVVWDMNCLEQVCGICTMIIN
                     GKVRQSCSALVDQLEQPITLEPMTKFPNVRDLAVDRTRMFEHLKQVKAWIEIDGTHDL
                     GPGPKMTQDEVAERYAYSRCMTCGCCLEACPQYDGDQYIGPQAIAQVRYQNMHPSGKM
                     TRDERLEGIMGDDGITNCGNAQNCVRVCPMSIPLTKAIYEENRETILHGLFSWFKR"
     gene            complement(5454..7481)
                     /gene="sdhA"
                     /locus_tag="VGB76_06360"
     CDS             complement(5454..7481)
                     /gene="sdhA"
                     /locus_tag="VGB76_06360"
                     /inference="COORDINATES: protein motif:HMM:NF006392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY0098559.1"
                     /translation="MAATSKGTKIAIVGGGLAGLSSAMKIAEAGHEVDLFSVVPVKRS
                     HSVCAQGGINGAVNTKGEGDSTWEHFDDTVYGGDFLANQAPAKAMCEMAPAIIYLFDR
                     MGVPFSRTKEGLLDFRRFGGTKHHRTAFAGASTGQQLLYALDEQVRRYEVGGKVKKYE
                     GWEMLSLVLDDHQVCRGLVALNRHTLELKAFPADAVIMATGGPGLIFGKSTNSMVCTG
                     SAVSACYQQGARYANGEFIQVHPTSIPGEDKLRLMSESARGEGGRVWVPRQKNDKRAP
                     ASIPEKERIYFLEEKYPAYGNLVPRDIATREIFQICLDGMGVGGENQVYLDLTHIDAA
                     TLDRKLGAILEIYEMFVGDDPRHVPMRIFPGVHYSMGGLWVDANQRTNIPGLLAAGEC
                     DYSIHGANRLGANSLVSCVYGGFIAAPAALEYAQNVERNGADGSKIYDDELKLQKSFN
                     DELLKQSGGENQHLLHDEMGKWMTDNVTVVRYNDRLKATDAKLQELIERYKRISISDS
                     NMWATMALPHARQLWNMLQLARVITLGALNRDESRGAHYKPDFPNRDDERFLKTTIAE
                     YSGEGPVLSYEEVDVSLIVPRKRDYSKGKQEQKTTGAVATQAAASGLPTTDGAQQVPV
                     EGWEQKPRDLTGPRVWGQDKEQVRDVNHGEAESRREQGKELKDAGGKSDEL"
     gene            complement(7481..>7871)
                     /locus_tag="VGB76_06365"
     CDS             complement(7481..>7871)
                     /locus_tag="VGB76_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098560.1"
                     /translation="WFYTIQRITGVILFFFILFHLLNFRFGLIPGLNERSVAHHPGEA
                     FEIVQREFQIWWILIIYVIGIASTVWHFANGIWLFLVDWGIVIGERAQRATGYACIGF
                     GVVLYVLWINVTFAFLRPGGLLGGILN"
BASE COUNT         1461 a         2430 c         2435 g         1545 t
ORIGIN      
        1 ctacggcgcg ctcggattaa agaaacgact ggactggcaa acaacctgcg accgtcgttc
       61 gccgcgctgc accacgcgct gggatgaatt gctcacactg aatctgaatt ccaccccgtc
      121 ttaatatttt tcgatcttga gatgtgcgac ggttgcgccc cagccgccgg attggggcgg
      181 cgcgtccgtc cactcgcgga cgaaaggcgt gcgcgcgagg atgctctgca cgaggcgacg
      241 ctgcacgccc tgtcccttgc cgtgaatgat gcgcacgctc aggaatcccg cgcgatgcgc
      301 ctccgttaaa tactcttgca cgacgcgctc gacatcgcgc ggcgcgatgg tgtgaagatc
      361 gaaaacgtcc gtgatctcca gcctgaccgg ctccggaaac ggattgtcga gatcaatttc
      421 gccctcctcc acgtcagacg ccgcgccgcc cgtcgcgctc gattcgccgc ccacgcgtgc
      481 ggcctcgcgg cgcgcggcaa acagagtttt taaccagtcg aataatggac tcatgcgtgc
      541 gcgaaagact cccgcgcaag tcttagcata ccttgacact cgccgctagc gcgccgtaaa
      601 ctcgcgcact tccaagtcat aaaattttgg caggccacaa attttcgtgt tgattggtgt
      661 gcgcgcccct cgggcgcagg caagattgat ctctaccggc aaaaattccg tctcaagttt
      721 gaaaggatga aaagcgatgg gtaagcgaaa caagatcact gtggtgggcg cgggcaacgt
      781 cggggcgacc gccgcgcact ggctggcgag caaggaactg ggcgacgtgg tgctcgtgga
      841 catcatcgag ggcatgccgc agggcaaggc gctcgatctg gcgcaggccg cgcccgtcga
      901 aggcttcgac gcgcgcatga tcggctcgac gaacgactac tcgctgacga aggactcgga
      961 cgtggtgatc atcacgtcag gacttgcgcg taaaccgggc atgagccgcg acgacctgct
     1021 caagacgaac gccgggatcg tctcgtcggt cgtggacgaa gtggtcaagt attcgcccga
     1081 ctctatcctg atcatcgtct ccaacccgct cgatgcgatg tgccaggtgg ctctgcgccg
     1141 ttcgggtttc ccgaagcatc gcgtgatcgg catggcgggc gtgctggact cggcgcgcat
     1201 gcgctgtttc ctcgccgaag cgttgaacgt gtcggtggag aacgtgacgg ccttcgtgct
     1261 cggcggccac ggcgacacga tggttccgct gccgcgctat tcgacctgcg cgggcatccc
     1321 cgtcacagag ttactgtcgc aggaacagat tgaatcaatc gtgaaacgca cggccaacgg
     1381 cggcgcggag atcgtcggcc tcttgaagac cggctcggcc tattacgcgc cctcgtccgc
     1441 gagcgtcgag atgacggaag cgatcttgaa agacaagaag aagattctgc cgtgcgcggc
     1501 ttatctcgaa ggcgagtacg gcatcaacgg cctctacgtc ggcgtgccct gcaaactcgg
     1561 ggcgaacggc ctcgaacaga tcatcgagat caacctgacg gctgaagagc gcatcgcttt
     1621 gcagaagagc gcggcgtcgg tgcaggaact cgtggacgtg ctcggcctct aagaacctac
     1681 gacgagcacg gaataattgt aggggcgggg cgtgtccctg cccctacaaa ttttttagga
     1741 gaacttcgtg cgctcacttc aagtctccct ccgtctggcg tgctgcttat tattcctgtc
     1801 acaacttgtc tgcgctcaaa acggcgtgtc tcaaaagaac gcgcccgccg ctgtcgccaa
     1861 atttgcattt cgtaaaagcg ggcttgaact cgaacgcgca gcccgcgcgg gcgcgttcta
     1921 cagcgtgacg gggcgacgcg cagccgcctt cggctatgag catcgcccgc tcgaagcgtg
     1981 ggtctacccg ttgaagattc tggacgactt tgcgttgtcg ttcaatatcg aaggctatcc
     2041 gctggagttc ggcggcgcgg acacgctcgc gcgcatcaac gtccgccccg aagccacgac
     2101 cttcacttac tcgcacgccg ccttcaccgt ccgccagacg atctttgcgc cgctggatga
     2161 gccgggcatc gtcatgctgc tcgacgtgca aagcgcgctt cccctgaccg tcaacgtctc
     2221 gtttcgcccg cgcctgcgcc tgtcatggcc tgccggtttg atgacgggca gcctgagttg
     2281 gaacgagaag tcgcgcgtct attacatcac ggaagagacg cgacgcttca cgggtatcgt
     2341 cggctcgccc gccgcgacgg acgtttcggt gatgccttat caggaggagc cgcgcggcgt
     2401 gcccgcgcgt tttcgcatcc cggttccggc gggcgagatg agttccggtt tcatcccgat
     2461 catcatcgca gggagcgtgg aaggacgcga gaaggcaaaa gccgcttacg accgtctgct
     2521 ctccgctatc ccgtcgctct acgaaaaaaa cgttgcgcat tatgagcggc tcgaagcgga
     2581 gacgctcggc atctcgacgc cggacaggga actcgacgaa gcgttcgcct gggcgaaggt
     2641 cggcgtagac aaaggcgtgg tgacgaatcc gctgctgggg acgggactcg tcgcgggcta
     2701 tcgcacgtcg ggcgaatcgg agcgacccgg cttcgcctgg ttcttcgggc gcgacgcgct
     2761 ctggactgcg tttgcactca actcctacgg cgacttccag acgacgcgca ccgcactcga
     2821 attcctgaaa aagtttcaac gcgccgacgg caaaatcccg cacgagattt cgcagagcgc
     2881 gtcgctcatt ccctggttca cagattacga atacccctgg gcgagcgcgg atgccacgcc
     2941 gctctacgtt atcgcgcacg ccgactattt tcgcgccaca ggtgatctcg aattcctgcg
     3001 cgccaactgg gactcgatcc tgaaagccta tcgcttcacc gaagcgaccg atacggacgc
     3061 gaacggcctc gtcgaaaaca cgaagttcgg gcacggctgg gtggaaggcg gcggactcta
     3121 tccggcgcac gaagagattt acatgcaggg tttgtgggtt gccgcctcgc gcgacctggc
     3181 cgggatggcc gccgctttga aggaggggaa agtcgccgcg ctcgcacgcg ccaacgccga
     3241 gcgcacgcgc gccgcgacgg aggagactta ctggttgccg cagcgcggct tctacgcttt
     3301 cgccaccaag cgtcctgcca cggagccgcc cgaagccgac cccggaccca atcgcgcaca
     3361 gcgtcaagcg cgcatgaatg cgctcaaaga tgcacggctt tatgacgaag atacagtgct
     3421 ccccgccgtc ccgctctggt ttaagacttt gacggacgaa cgcgccgaac ttcaactcga
     3481 cgcgctcggc gcgggcgcgc ttgcgaccga ctggggcgcg cgcatcctct ccaaccaaag
     3541 ccaactctac gatccgctct cttatcacaa cggctccgtc tggccgctct tcacgggttg
     3601 ggcgagcctg ggcgcgtacc gttacggtcg cccgcacgtc ggctttcaag ccctgatggc
     3661 gaatgcgctg ctcacgcgtc agggcgcgga aggctccgtc acggagttgt tgtccggcga
     3721 cttcaacgcg ccgttcggtc gttcatcgca ccatcaggtc tggtcggagg cgatggtcgt
     3781 cacgccgctc gtgcgtgggc tgctggggct ggaaccggac gcggcggacg atgacgtttt
     3841 gcgtctcgcg ctgcaactgc ccgtcaactg ggacagactc gaagtgcgca acctgcgcct
     3901 cgcgcgcggc tactacgata tgcgcctcgt gcgccggacg ggacagacca ggttgagttt
     3961 tattccgcac cgcgccagcc aactcgccaa cgcgaaaggt agcgcggaag ggctgaggaa
     4021 gatcgccgtc tcgatctctc tgccgctcga cgcgcgaatg tgccgagtca cgaagccccg
     4081 gctggcgggc agttccctgg aacagcagcc gactgcgccg gtggattgtg atgcgcggac
     4141 gtacaagttg acgcgcatgg gcgatgtgca acgcgtcgag gcgatgttcg ttcccgaagc
     4201 gtcggaggtg ggtcttgttt tttcctatga agagggaacc gacgtttatg tcgcgcctga
     4261 gacgcctgtg gcgggcgcac gtaatcgcgg cctgcgcatc ctgcgttcgc gcgcagatgc
     4321 gagccacctg cgcctcctcg tcgaaggatt aggcggccag acctacacgc tcggcacgcg
     4381 cagcagccgc gcgttgctcg ctacctccgg cgtggagatc aaaaaaggga ccgacgccgg
     4441 ttcacaattg ctgatcactt tcgccggaac gcccgacagg tatgtgcgac gcgaaataga
     4501 catcccgcta caagcgtcga agccgggacg acggaattag cccgcgcgga gttgatacga
     4561 aggggcgaca cgaaaaagcg gcgacgcgta aatcaagttt acgcgtcgcc gctttgttcg
     4621 acgtttgaga gttcagcgtt tgaaccagct gaacaacccg tgcaagatcg tctcgcggtt
     4681 ctcttcgtag atggctttcg tcagggggat ggacatcggg cagacgcgga cgcagttctg
     4741 cgcgttgccg cagttggtga tgccgtcgtc gcccataatg ccttcgagcc gttcgtcgcg
     4801 cgtcatcttg ccggaggggt gcatgttctg gtagcgcacc tgcgcgatgg cctgaggtcc
     4861 gatgtactgg tcgccgtcgt attgcgggca ggcttcgagg cagcagccgc acgtcatgca
     4921 gcgcgagtag gcgtagcgtt cggccacctc gtcctgcgtc atcttcggtc ccggtcccag
     4981 atcatgcgtg ccgtcaatct cgatccacgc tttgacctgt ttcagatgtt cgaacatgcg
     5041 cgtgcggtca acggcaagat cgcgtacgtt cggaaacttc gtcatcggct ccagagtgat
     5101 cggctgctcc aactgatcca cgagcgcgga gcacgactgg cgcaccttgc cgttgatgat
     5161 catcgtgcag atgccgcaga cctgttccag gcagttcatg tcccagacga cgggcgtggt
     5221 cttcgtgccg tccttcgtga cggggttttt ttgaatgtcc atcaggcacg agatgatgtt
     5281 gaggttgggg cgatagggga cgcgaaactc ttcccaacgc tgcggcgcat cggggcggtc
     5341 gcgtcgcttg atgcgaatct cgacgaactt ctgcgccctc tgctgctcag gcgcaggtgt
     5401 gtacgacctg tcggcggtcg cctccgtgct gttggtttga ttagtgtgtg cgctcatagc
     5461 tcgtctgatt tgccccccgc gtctttgagt tccttaccct gctcgcgccg cgactcggcc
     5521 tcgccgtggt tcacgtcgcg cacctgctct ttgtcctgac cccagacgcg cggccccgtc
     5581 aggtcgcgcg gcttctgctc ccaaccttcg accgggactt gctgcgcgcc gtcggtcgtc
     5641 ggcaggccgg aggccgcagc ctgcgttgcg accgcgcccg tcgtcttctg ctcctgcttg
     5701 cccttgctgt agtcgcgctt gcgcggcacg atcaaagaca cgtcaacctc ttcgtaagag
     5761 agcacgggac cttcgccgga atactcggcg atggtggttt tgagaaaacg ttcgtcgtcg
     5821 cggttgggga agtccggctt gtagtgcgcg ccgcgcgact cgtcgcggtt gagcgcgccg
     5881 agcgtgatga cgcgcgcgag ttggagcatg ttccagagtt gacgcgcatg cggcagagcc
     5941 atcgtcgccc acatgttcga atcgctgatg gagatgcgct tgtagcgttc gatcaactcc
     6001 tgcaacttcg cgtcggtcgc tttcaacctg tcgttgtaac gcacgacggt gacgttatcc
     6061 gtcatccact tgcccatctc gtcatgcagg agatgttgat tttcgccgcc cgactgcttg
     6121 agcagttcgt cgttgaagct cttttgcagt ttcaactcgt cgtcgtaaat cttcgagccg
     6181 tccgcgccgt tgcgctccac gttctgcgcg tattcgagcg cggcgggcgc ggcgatgaag
     6241 ccgccgtaga cgcaactcac gagcgagttt gcgccgaggc ggttcgcacc gtgaatcgaa
     6301 tagtcgcact cgcccgccgc gagcaggccg ggaatgttcg tgcgttgatt cgcgtcaacc
     6361 cagaggccgc ccatcgaata atggacgccg gggaaaattc gcatcgggac gtggcgcggg
     6421 tcgtcgccga cgaacatctc gtagatttcg agaatcgcgc cgagcttgcg gtcgagcgtg
     6481 gcggcgtcaa tgtgcgtcag atcgagatag acctgattct cgccgccgac gcccatgccg
     6541 tccagacaga tctgaaagat ttcgcgcgtg gcgatgtcgc gcggcacgag gttgccgtaa
     6601 gccggatatt tctcttcgag aaaatagatg cgctcctttt caggaatgga cgcgggcgca
     6661 cgcttgtcgt tcttctggcg cggaacccac acgcgcccgc cctcgccgcg cgccgattcg
     6721 gacatcaggc gcagcttgtc ttcgcccgga atcgaggtcg ggtgaacctg tataaactcg
     6781 ccgttggcat aacgcgcgcc ctgttgataa caggccgaaa ccgcgctccc cgtgcagacc
     6841 atcgagttcg ttgacttgcc gaagatcaaa ccggggccgc ccgtcgccat gatcaccgcg
     6901 tcggcgggaa aggctttgag ttcgagcgtg tggcggttca gcgcgacgag gccgcgacaa
     6961 acctgatgat cgtccaagac gagcgacaac atctcccagc cttcgtactt cttcactttg
     7021 ccgccgactt cgtagcggcg cacctgttca tcgagcgcgt agagaagttg ctgcccggtt
     7081 gatgctccgg cgaacgccgt gcggtggtgc ttcgtgccgc cgaagcgtcg gaagtcgagc
     7141 agcccttcct tcgtgcgcga gaagggcacg cccatgcggt cgaagagata gatgatcgcg
     7201 ggagccatct cgcacatggc tttggcgggc gcttgattgg cgaggaagtc gccgccgtaa
     7261 accgtatcgt cgaagtgttc ccacgtcgag tcgccttcgc ccttggtgtt gaccgcgccg
     7321 ttgatgccgc cctgcgcgca gaccgaatgg gaacgcttga cgggcacaac cgagaagaga
     7381 tcaacttcgt gtcccgcctc ggcgatcttc atcgccgagg agagtcccgc caagcccccg
     7441 ccgacaatcg caatctttgt tcctttagaa gtcgccgcca ttagttaagt attcctccca
     7501 gaagcccgcc ggggcgcagg aaagcgaagg tcacgttaat ccacagaacg tagagcacga
     7561 cgccgaagcc gatgcaggca tagccggttg cacgctgcgc gcgctcgccg atgacgatcc
     7621 cccagtcaac taaaaagagc cagatgccgt tggcgaagtg ccagacggtt gaggcgatgc
     7681 cgatgacgta aatgatcaga atccaccata tctgaaattc gcgctgcacg atctcgaaag
     7741 cctcgcccgg atgatgcgcg accgagcgtt cgttgaggcc ggggatcaga ccgaaacgga
     7801 agttcaggag gtgaaagaga atgaagaaga agagtatcac gcccgtgatg cgctggatcg
     7861 tgtagaacca g
//
LOCUS       DASWNS010000187         8704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_397001, whole genome shotgun sequence.
ACCESSION   DASWNS010000187 DASWNS010000000
VERSION     DASWNS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397001"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1306)
                     /locus_tag="VGB76_06370"
     CDS             complement(242..1306)
                     /locus_tag="VGB76_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY0098561.1"
                     /translation="MKMGFIPKCLALVVLAGAALLSLSQPTGAQPAQTAATMTSAPAA
                     NDAALLVLNKAENTLAIVDPVALKVLARVPVGEGPHEVVASADGRTAYVGNYGTQQVV
                     GSSLSIIDIAARKETKRVDLGALRRPHGIVEAGGKIYFTAEANRAVARYDPATDRVDW
                     LMGTGESVSHMLVITPDAKRIYTANMLSNTVTAINVGGAPVPPNITQIAVGKTPEGIG
                     ISPDGREVWAANRADGTVSVIDTTTNKVVETLQKFSQLPFRVAFTPDGKRVLIPDPVG
                     GELVVFDAATRREIKRIKIEGGTVGVTVAADSRRAYVALQEANGVAAIDLEKLEVVGR
                     VETGKAPDGIAWAQRKETAK"
     gene            complement(1471..2070)
                     /locus_tag="VGB76_06375"
     CDS             complement(1471..2070)
                     /locus_tag="VGB76_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098562.1"
                     /translation="MNAWLKRLEVPIAAALVLAGAAFLLKMCYLPFVLCTSLGAAWLA
                     AVYLYLRARYEILMPPVLLLLVFAALEVDALGNLFSLYGRTFAGVIMYDEFAHLAVQA
                     LTMPLVVWLLSRALSRFGHALPLSLTICFAVAIFFSLSAFYEIIELWDERYFGGQRIW
                     SKHDAPNDLQWDFAGILIGALLAYLILRRARNFEVALAT"
     gene            complement(2336..2827)
                     /locus_tag="VGB76_06380"
     CDS             complement(2336..2827)
                     /locus_tag="VGB76_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098563.1"
                     /translation="MKIEDINFEPHKGVAIFTTGLSVIFTLVFGTISVVNLYRQLRDP
                     VSTMDIFGWFFTFFITFTCSALLLYFSTAIYRLAFYESGIVILGLRGRRFVPWSAVRD
                     ARINRFKGNIELALRAEGRRLPVSVPLNGYKKQLTLLAELRRRLPVAINDPSNIAATL
                     LDN"
     gene            2956..3753
                     /locus_tag="VGB76_06385"
     CDS             2956..3753
                     /locus_tag="VGB76_06385"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY0098564.1"
                     /translation="MADLFQPTILRYDSLPSTNTEAASQAARGAPEGLCVVAREQTRG
                     RGRQERAWASPPDAGLYFSVVLRPRLKLEAWPLITLLAALAVHDALREACALATDIKW
                     PNDILAATTTTGGGGGRKLCGILAETVETKTGRACILGIGINLSERAYPPELAAHATS
                     IEGATGNAPDLERVLAALCKHLARRYAQLHAPATGAGEIIRDWSANSSYAEGRRVRVH
                     AATEVFTGTTRGLEPDGALRVETATGEIRIVRAGDVHALRTETMNAE"
     gene            3823..4065
                     /locus_tag="VGB76_06390"
     CDS             3823..4065
                     /locus_tag="VGB76_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098565.1"
                     /translation="MARIKGVEAQAVDGYIARVFAAQAKRWGAPLLNHLLYARRPAIF
                     RGVRAMWNGIEASGLIDAKLQTLVNRRVARLNGCEF"
     gene            4195..4380
                     /locus_tag="VGB76_06395"
     CDS             4195..4380
                     /locus_tag="VGB76_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098566.1"
                     /translation="MTITGREVSDELFTRLREFYDDDALVELTAIIAWENASSKFNRA
                     LRVPSQGLWKSSDEKDK"
     gene            4473..5786
                     /locus_tag="VGB76_06400"
     CDS             4473..5786
                     /locus_tag="VGB76_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEY0098567.1"
                     /translation="MKSRRIVFSTFGSFGDVHPYVAVALELKARGHHPVIATSEIYRE
                     KAEALGIEHHPVRPVVPSYDEPDEIAPLIEKVMDARTGTEEVLNTFLLPHLRDIYDDL
                     SAAVRGADLLVTHPLSMPGIIVVEKTGVPWVSSVLAPGSMLSVYDPPVPPQLPALHSL
                     MTFHTLISRAVMRLGRWKTASLAQALNSLRADVGLPPSSAHPLFEGQHSPRLVLALFS
                     RVLAEPQPDWPPHTRITGFPFYDRRDFGGDREDDDAGGGLSSELRRFLDAGEPPVVFT
                     LGSSAIWVARDFYRESVAAARALGQRALLLIGHARNRLAEPLPEGVAAFEYAPYGELL
                     PRARAVVHQGGVGTTGQTLRSGRPALVVPFSHDQFDNGARVARLGCGRMLARTKYNAA
                     SATRELRAILSDESYKTMAREVGWRVQAEDGTRAASDAIEEVLNG"
     gene            5956..6525
                     /locus_tag="VGB76_06405"
     CDS             5956..6525
                     /locus_tag="VGB76_06405"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0098568.1"
                     /translation="MQQTQFTPLESERLILRRFNEADLAPFLDYLNDPLVARYQTWES
                     YTEQAAREAIESQQGLDPGVPGKWFTFALELKETGALAGHVALGVRVETPQQAEIGVT
                     LARAFHGQGLAFEAARRVLDYAFDELALHRVTAVTDCENANSIALTERLGMRREGHFI
                     QNIWFKGKWGDEFQYAVLRDEWLQRRRAV"
     gene            6591..7355
                     /locus_tag="VGB76_06410"
     CDS             6591..7355
                     /locus_tag="VGB76_06410"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY0098569.1"
                     /translation="MLLVIDVGNTNTSLGVYDGERLVTHWRLTTERGRTVDEYGVLSR
                     NLFALAGLDFKEIDAVAAASVVPQLNFTVKRMAEEYFQLSPLFIDHTTDTGMEILYQP
                     PSDVGADRIVDAVAARHKYGAPVIVVDFGTATTFNAVNALGQYLGGVITPGIQISSDA
                     LFARAARLPRVEIKRPQAVIGSSTNGAIQSGLYYGYVGLVDGILRRMHEELAGRVRVV
                     ATGGLASLIATGSELIETVDDTLTLEGLRLIYEKQG"
     gene            7522..8271
                     /locus_tag="VGB76_06415"
     CDS             7522..8271
                     /locus_tag="VGB76_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098570.1"
                     /translation="MNYFNYFTEIEDAFIRRRGKHLLLSPMDWALIEGWKARGVPLHI
                     VLRGIEQSFDSYEAKPRKRSVKSLFYCQEEVEAQYAEWLESQVGATSAETDAAGKTPA
                     KNTHAETGNGALPFPREVIAGHLAESRAQLERACEEHRKRGAGELCEAVGRAAARLSA
                     LEKDFAAAARPDAEQLEGALNDLENLLDRALRADLAPERLNARRAEAAAQLEPYRKRM
                     ERATYEQTLDSLLAKLLREEGGLPRLSLFYL"
     gene            8295..>8704
                     /locus_tag="VGB76_06420"
     CDS             8295..>8704
                     /locus_tag="VGB76_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEY0098571.1"
                     /translation="MKRQQRHRRPRTDAPRVEGALSVGAQLEVEIERIVPGGAGLAHA
                     AGYTLFVTLAAPGDRVRVNVERVRGKTAFASVVEIVKPSPVRVEPPCPYFGRCGGCDF
                     QQLTYVAQLQAKVEIIRDCLRRIARIDPPADIPIT"
BASE COUNT         1679 a         2693 c         2698 g         1634 t
ORIGIN      
        1 gtcagccgtg taatcgtgtg cccgccatgt cggttattgc gcatcgggca cgacttctat
       61 catgcgatag aagtgtgtag caactgaaag tcttgcaccg aaggtgcata gttttgcact
      121 agcgtgtctg tgacaataaa aagcaggagt cagaattcag aatccagcag tcagaagaaa
      181 acatctgccg cccttcattc tgactcctgt attctgtttt ctgtcttctc cttcatcgtt
      241 cctacttcgc cgtttccttt ctttgcgccc atgcgatgcc gtccggcgct ttacccgtct
      301 cgaccctgcc tactacttcc agtttttcca gatcgatggc ggccacgccg ttcgcctcct
      361 gcaacgcgac ataagcgcgc cggctgtcag ccgcgacggt gacgccgacc gtcccgcctt
      421 cgatcttgat gcgcttgatc tctctgcgcg tcgcggcatc gaagacgacg agttcgccgc
      481 ccaccggatc ggggatgagc acgcgcttgc cgtcgggcgt gaaggcgacg cggaagggaa
      541 gttgcgaaaa cttctggagc gtctccacga ccttgttcgt cgtcgtgtca atgaccgaga
      601 cggtgccgtc ggcgcggttg gccgcccaca cttcccgacc gtcgggcgag atgccgatgc
      661 cttcgggcgt cttgccgacg gcgatctgcg tgatgttcgg cggcacgggc gcgccgccca
      721 cgttgatcgc cgtgacggtg ttcgagagca tgttggcggt ataaatccgc ttcgcgtcgg
      781 gcgtgatgac gagcatgtgc gagacgctct cgcccgtgcc catcagccag tccactctgt
      841 ccgtcgccgg atcgtagcgc gcgacggcgc ggttggcctc cgccgtgaaa tagattttgc
      901 cgcccgcctc cacgatcccg tgcgggcgtc ggagcgcgcc gagatcaacg cgcttcgtct
      961 ccttgcgcgc cgcgatgtct atgatcgaga gcgaactgcc gacgacctgc tgcgtgccgt
     1021 agttgccgac gtaagccgtg cgcccgtcag ccgaagcgac aacctcgtgc ggcccttcgc
     1081 cgacgggcac gcgcgccaag accttcaacg ccacagggtc aacgatggcg agcgtgttct
     1141 ccgccttgtt gagcacgagc agcgcggcgt cgttggcggc gggcgcactc gtcatcgtgg
     1201 ccgcagtttg cgccggttgc gcgccggtcg gttgagacag agagagcaac gccgcgcccg
     1261 ccagcacgac gagcgcgaga cacttcggga tgaatcccat cttcatggat caagtctcca
     1321 ttctttcgga atctaaatcg ggacggaacg gcagacgcct gcggcaagtt aatacgtaaa
     1381 agcgcggggc gtgtttcgcg tcgcggatga gaaattcgaa ctgtgccggg attgcgtcac
     1441 tgcattggga ggctgacatc gccgcgcgcc tcaagttgcc agcgcgactt cgaagtttct
     1501 cgcgcgccgc agaataaggt aagcgagcag cgcgccgatg aggattccgg cgaagtccca
     1561 ctggaggtcg ttgggcgcgt cgtgcttgct ccagatgcgt tgaccgccga agtagcgttc
     1621 gtcccagagt tcgatgatct cgtagaaggc tgagaggctg aagaagatgg cgacggcgaa
     1681 gcagatggtg agcgagagcg gcagcgcgtg gccgaaacga ctcaatgctc ttgacagcag
     1741 ccacacgacg agcggcatcg tcaaagcctg caccgccaga tgcgcaaact cgtcgtacat
     1801 gatgacgccc gcgaacgtgc gcccgtacag cgagaagaga ttgccgagcg cgtccacctc
     1861 caacgccgcg aagactaaga gcagcaacac gggcggcatc aggatttcgt agcgcgcacg
     1921 caggtagaga tagaccgccg ccagccacgc cgcgccgagc gaggtgcaga ggacgaacgg
     1981 caggtaacac atcttgagca ggaaggccgc gcccgcaagg acgagcgcgg cggcgatggg
     2041 aacttccaga cgtttgagcc atgcgttcat ctcatgctcc ttacgcgtcg ccgtcaggcg
     2101 cgggcggcgt cgcctgttca ctagccgcta acgcgcccgt cgttgattcg tcaactcgtt
     2161 aattcgtcac ttcgttgatt catcaacggc gcgctgcgcg aactgtttac tccagcacag
     2221 aacgtttgag gccgccgctt gatgcgtgaa atttgcgcga aaaatttttc atgcggacac
     2281 tcgtaacgaa aactcgtcgc gcccgttcga ctcgccgccg cccggtcggc gcgccttaat
     2341 tgtcgagcag cgtggcggcg atgttggacg ggtcgttgat cgctacgggc aggcgtctgc
     2401 gaagttccgc aagcagcgtt aactgttttt tgtagccgtt gagcgggacg ctgacgggga
     2461 gacgccgccc ttccgcgcgg agtgcgagtt cgatgttgcc cttgaagcgg ttgatgcgtg
     2521 catcgcgcac cgcgctccag gggacgaaac gccgcccgcg cagtccgagg atgacgatcc
     2581 cgctctcgta gaacgcgagg cgatagatgg cggtgctgaa atagagaagc agcgcggagc
     2641 acgtgaaggt gatgaagaag gtgaagaacc acccgaagat gtccatcgtc gaaacaggat
     2701 cgcgcagttg ccggtagagg ttcaccacgg agatcgtacc gaagacgagt gtgaagatca
     2761 cggacaaccc ggtcgtgaaa atggcgacgc ctttgtgcgg ctcgaagttt atgtcttcaa
     2821 ttttcatctg acgccacgcg cgctcccgtc cgtgtttgca ccgcgcgaca catttttcac
     2881 gcccgcgcgt tgtccgtcaa tccctgctcg cataagatga gtttgagatt gaagtgaggt
     2941 gagtttgaga ctgacttggc cgacctcttc cagccgacca tcctgcgcta cgattctttg
     3001 ccttccacca acacggaggc ggcgagtcag gccgcgcgcg gcgcaccgga ggggctgtgc
     3061 gtcgtggcgc gcgagcagac gcgcgggcgc gggcgacagg agcgcgcgtg ggcttcgccg
     3121 ccggatgcgg gtctgtattt cagcgtcgtc ctgcgcccgc gcctgaagtt ggaggcgtgg
     3181 ccgctcatca cgctgctggc cgcgctcgcc gtgcacgacg cgctccgcga ggcgtgcgct
     3241 ttagcgaccg acatcaagtg gccgaacgac atcctcgctg caacgacgac gacgggcgga
     3301 ggtggcggtc gtaaactctg cggcatcctc gccgaaaccg tcgagacgaa aaccgggcgc
     3361 gcgtgcatcc tcggcatcgg catcaacttg agcgagcgcg catacccgcc ggagttggcc
     3421 gcgcacgcca cttcaatcga aggcgcgacg ggcaacgcgc cggacttgga acgcgtgctc
     3481 gccgcgctct gcaaacatct cgcgcgtcgc tacgcgcaac tgcacgcgcc cgccacaggt
     3541 gcaggcgaga tcatccgcga ctggtctgca aattcttcct acgccgaagg gagacgcgtg
     3601 cgcgtccacg ccgcgacgga agttttcaca gggacgacgc gcggccttga gccggacgga
     3661 gctttgcgtg tagaaacggc gacgggcgag atcagaatcg tgcgcgcggg cgacgttcat
     3721 gcgctgcgca ccgaaacgat gaacgcggaa tgaaagcagt cctgctttcg ttttcagttc
     3781 atagttcatc gttcctgctt catcattcat agagagggtt ctatggcgag aattaaagga
     3841 gtcgaagcgc aagcggttga tggctacatc gcgcgtgtgt ttgcggcgca ggcgaagcgt
     3901 tggggcgcgc cgctcctgaa tcatctgctc tacgcgcgcc gcccggcgat ctttcgcggc
     3961 gtgcgcgcca tgtggaacgg catcgaggcg tccgggttga tcgacgcgaa actgcaaacc
     4021 ctcgtcaacc gccgcgtcgc acggctcaac ggctgcgagt tttgacagga catcaacgct
     4081 gcggtcggca gcgaactcgg cctcgcgaaa gagaaaattt tagctctcgc ggattacgcg
     4141 cacagcccgc tctacacgga ggccgaacgc gtcgcgcttg agtatgcgga agccatgacc
     4201 atcaccgggc gcgaagtctc ggacgaactc ttcacgcgcc tgcgcgaatt ttatgacgac
     4261 gacgcgctcg tcgaactgac ggccatcatc gcgtgggaga acgcttccag caaattcaac
     4321 cgcgccctgc gcgtgccctc gcaaggactt tggaaaagca gtgacgagaa agacaagtga
     4381 tgagtgatgg gtgatgagta atgagtgaga accaaagact tgtctttcac tcattactca
     4441 ttactcatta ctcatcactt cttccttata ttatgaagag caggcgcatc gtattttcta
     4501 ccttcggttc gtttggcgac gtgcatcctt acgtcgcggt ggcgctcgaa ctgaaggcgc
     4561 gcgggcatca tccggtcatc gccacgtcgg aaatttatcg tgagaaggcc gaggcattgg
     4621 gcatcgaaca tcatccggtg cgcccggtcg tgccgtctta cgacgagccg gacgagatcg
     4681 cgccgttgat cgaaaaagtg atggatgcgc gcacggggac ggaggaggtg ctgaacacgt
     4741 tcctcctgcc gcacctgcga gacatttacg atgatttgag cgcggcggtg cgcggggctg
     4801 atctgcttgt cacgcacccg ctctcgatgc cggggatcat cgtcgtcgaa aagacgggcg
     4861 tgccctgggt gtcgagcgtg ctcgcccccg gctcgatgct ctccgtctat gatccgcccg
     4921 tgccgccgca gttgcctgcg ctgcacagtt tgatgacgtt ccatactctc atctcgcgcg
     4981 ccgtcatgcg gctcggcaga tggaagacag cttcgctcgc gcaggcgttg aacagcttgc
     5041 gcgccgacgt gggcttgccg ccgtcgagcg cgcacccgct cttcgaaggg cagcattcgc
     5101 cgaggctcgt gctcgcgctc ttctcgcgcg tgctggccga accgcaaccc gactggccgc
     5161 cgcacacgcg catcacgggc tttcccttct acgaccgccg cgacttcggg ggcgaccgtg
     5221 aagacgacga cgctggcggc ggcctctcgt ccgaactccg gcgctttctc gacgcaggag
     5281 agccgcccgt cgtcttcacg ctcggctctt cggccatctg ggtcgccaga gatttctacc
     5341 gcgagtcggt tgccgccgct cgcgcgctcg gtcaacgcgc tctgctgctc atcggtcacg
     5401 cacgcaatcg tctcgcggaa cctctgccgg aaggcgtggc cgcgttcgag tacgcgcctt
     5461 atggcgaact gttgccgcgc gcacgcgccg tcgtgcatca gggcggcgtc ggcacgacgg
     5521 gacaaactct gcgctccggt cgccccgcgc tcgtcgtgcc gttcagccac gatcaattcg
     5581 acaacggcgc acgcgtcgcg cgtctgggct gcgggcgcat gctcgcgcgc acgaaataca
     5641 atgccgcgag cgcgacgcgg gaattgcgtg caatcctgag cgatgaaagt tataagacta
     5701 tggcgcgcga agtcgggtgg cgcgtgcagg ccgaagacgg aacgcgcgcc gccagcgatg
     5761 cgattgaaga agtgttgaac ggttaagggc gagcgacggc agaagatcgt tgtcgtggtt
     5821 tcaacttaac cctctctgcg cctctccgcg tttctctctc tagtctctgc gagtgagtgt
     5881 tggtcaaata agcattcacc gcagaggcgc acagaaccgc agcgggggcg cagaggatga
     5941 atcaaatgag gcgagatgca gcagacacaa ttcactccgc tggaatcgga gcgtctaatt
     6001 ctcagacggt ttaacgaagc agacctcgcg ccgtttctag actatctcaa cgacccgctc
     6061 gtggcgcgct atcaaacgtg ggagtcgtac acggagcagg cggcgcggga ggcgattgaa
     6121 agtcaacagg gactcgatcc cggcgtgccc ggaaagtggt tcaccttcgc actcgaattg
     6181 aaagagacgg gcgcgctcgc cggtcatgtc gccctcggcg tgcgcgtaga gacgccgcaa
     6241 caggccgaga tcggggtcac gctcgcgcgc gcgtttcacg gccaggggtt ggctttcgag
     6301 gccgcgcggc gcgtgctcga ttacgcgttc gacgaactcg cattgcaccg cgtcacagcg
     6361 gtaacggatt gcgaaaacgc gaattccatc gcgctcaccg aacggctggg gatgaggcgg
     6421 gaaggccact tcattcaaaa tatctggttc aaaggaaagt ggggcgacga gtttcaatat
     6481 gcagtcctgc gcgatgaatg gctccaacga aggcgcgccg tttaacctcg catcgccgcg
     6541 agttttttta actcgtcact tgtcacccgg cacacgtcac ttttaaagat atgctacttg
     6601 tcatcgatgt cggaaacacc aacacctcgc tcggcgtgta tgacggcgag cggctcgtca
     6661 cgcactggcg tctgacgacg gaacgcggac gcacggtgga cgaatacggc gtgctctcgc
     6721 gcaacctgtt tgcgctggcg gggctggact tcaaggagat cgacgccgtg gccgccgcct
     6781 ccgtcgtgcc gcagttgaac ttcacggtca agcgcatggc ggaagagtat ttccaactct
     6841 cgccgctctt catagaccac acgaccgaca cggggatgga gattctctat caaccgcctt
     6901 cggacgtggg cgcggacagg atcgtggatg cggtcgccgc ccgccacaag tacggcgcgc
     6961 cggtcatcgt cgtagacttc ggcacggcga ccactttcaa tgccgtcaac gcgctcggac
     7021 aatacctcgg cggcgtcatc acgcccggca tccagatctc ttccgacgcg ctcttcgcac
     7081 gcgccgcgcg cctgccccgc gtcgagatca aacgcccgca agccgtcatc ggctcttcca
     7141 ccaacggcgc gatccagtcc ggcctctact acggctacgt cggactcgtg gacggcatcc
     7201 tgcgtcgcat gcacgaagaa ctcgcaggcc gcgtccgcgt cgtcgccacc ggcggactcg
     7261 cctcgctcat cgccaccggc tcggaactca tcgaaaccgt tgacgacacg ctcacgctcg
     7321 aaggactcag attgatctac gagaaacagg gctgaaagag agtgacaagt gacaagtgac
     7381 gagtgacaag ttaaaaagac gtaaatcgta aatcgtgaat cgcagatcgt gaatagactc
     7441 gaaacgggtt ttctctcttc acgatttacg attcacgatt cactgtttta atctcgtcac
     7501 ctgtcacttg tcactgttct tatgaattac ttcaactact tcacggaaat cgaagacgcg
     7561 tttatccggc gacgcgggaa acacctgttg ttgagtccga tggattgggc tttgatcgag
     7621 ggttggaagg cgcgcggcgt gccgctgcat atcgtcttga gaggcatcga gcagtcgttc
     7681 gattcttacg aggcgaaacc gcgaaagcgc agcgtcaagt cgctctttta ttgtcaggag
     7741 gaggtcgagg cgcaatatgc ggagtggctc gaatcgcagg tcggcgcgac ttctgcggag
     7801 acggacgcgg cggggaaaac tcctgcgaag aatacgcacg ccgagactgg aaacggcgcg
     7861 ttgcctttcc cgcgcgaagt gatcgcgggg catctggcgg agagccgcgc gcaattggag
     7921 cgcgcctgtg aggagcatag aaagcgcggc gcgggcgagt tgtgcgaagc ggtcgggcgt
     7981 gccgccgcgc gtctctccgc gctggaaaaa gatttcgccg ccgccgcccg acccgatgcc
     8041 gagcaactcg aaggcgcgct caacgatctg gaaaacttac tagaccgcgc cctgcgcgcc
     8101 gacctcgcgc ccgaacggct caacgcgcgc cgcgccgaag ccgccgcgca actcgaaccc
     8161 tatcgcaagc gcatggagcg cgccacctac gaacagacgc tcgatagcct gctcgcaaaa
     8221 ctcctgcgcg aagaaggcgg cctgccgcgc ctgagcttgt tctatctcta acccgcgcga
     8281 gataattccc acacatgaaa aggcaacagc gacaccgtag gccgcgcacg gatgcgcccc
     8341 gcgtcgaagg cgcgttgagc gtgggcgcgc aactcgaagt cgagatcgaa cgcatcgtgc
     8401 cgggcggcgc gggtctggcg cacgccgccg gatacacgct cttcgtcacg ctggccgcgc
     8461 ccggcgaccg cgtgcgcgtc aatgtcgaac gcgtgcgcgg caaaacggcc ttcgcctccg
     8521 tcgtcgagat cgtcaagcct tcgcccgtgc gcgtcgaacc gccctgccca tacttcggtc
     8581 gctgcggcgg ctgcgacttc cagcagttga cctacgtggc gcaactgcaa gccaaagtcg
     8641 agatcatccg cgactgcctg cggcgcatcg cgcgcatcga cccgcccgcc gacattccca
     8701 tcac
//
LOCUS       DASWNS010000188         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_190235, whole genome shotgun sequence.
ACCESSION   DASWNS010000188 DASWNS010000000
VERSION     DASWNS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190235"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..869)
                     /locus_tag="VGB76_06425"
     CDS             complement(252..869)
                     /locus_tag="VGB76_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098572.1"
                     /translation="MKRKYQLLLIAALLLSTQLASVALAQRSRTIAVPVPPPRGASLP
                     PPTGTNAPVAPKVDAPKSVKAKYEGGIFGYNKKQTGTLNFDDEGRRLVFRDKAQKEYI
                     SIPYKSIAAAYADTQSRRPTAASVLGGASIYTLPALLIRKKYRYLTMQFSDPDTRVSG
                     VTSFKMDNKELLENILAAVAYKAELSPRGEAFVRLNKPQDEDQDK"
     gene            complement(917..1510)
                     /locus_tag="VGB76_06430"
     CDS             complement(917..1510)
                     /locus_tag="VGB76_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098573.1"
                     /translation="MIKIAKIVKSNSHVDYVGRVIDRLDVDAPPDSSDYGFAQFVSLP
                     VADNFDVVGIAYNTQLVNPEYGQFGPRLSPAADLAILSPDYLNEQGVLIGILLLGWRE
                     DGRTQMSVPRRVIPVGQDVFRLDEAQMEEFHRDGEGRVGLHYYSQIVAHAGAFAVPLV
                     EAILDQLDDVCTPEERQRFCVLKRSLAWQRTLGGVRL"
     gene            complement(1597..2106)
                     /locus_tag="VGB76_06435"
     CDS             complement(1597..2106)
                     /locus_tag="VGB76_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098574.1"
                     /translation="MKQSHEQMFDGEANRSREASAVVCPACGVAARRAGAHFCATCGR
                     GLRERAYAPADSLLASYHQQHSRPAMSVEQEMSGVRVQRTPRFSLPPHGGNTSAAIAL
                     VMVCCSLFPLYGIMFSPFVIVFGGFGLIEAWLFRRMGGARLVVTCIAGGLLIAGAQVL
                     VWLYILSYL"
     gene            complement(2177..3442)
                     /locus_tag="VGB76_06440"
     CDS             complement(2177..3442)
                     /locus_tag="VGB76_06440"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEY0098575.1"
                     /translation="MLYRPHLNAQLKERRAEFVHFEAVWNASVGEYARRLRALGGRAA
                     GEIKLEAERAITAGAARAPGALPSAELEGARSFVVPFAERWRSHEEARRWALEVLRER
                     VTCAADGSQILPGREISLPVAAVQAAWFENPHTAGGQDSYRKESRFAIITPQELLEAE
                     GGAATAADLVGLRRFELELRALTEFLERRRGWRARGERLPVAFFDGTLLLSTTRARAE
                     TLDFPRAYIQTIVEAVELSRDTEVPLVGYIDQSYARDLVRLLDLLSEEARPAATVFDA
                     RLLSITNAAEAATNGGNGESLFGAWGDRTIFCYCVREGMQDFQDEEGNPLVGFVYLQT
                     TGEGSPARLDIPAWVYEAGLVDEVVDTVRAECVVGNGYPYALETADAAAVITMQDREQ
                     FLRAMQEFAAEQKLGFRVSRKPISKAHRR"
     gene            complement(3541..4074)
                     /locus_tag="VGB76_06445"
     CDS             complement(3541..4074)
                     /locus_tag="VGB76_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098576.1"
                     /translation="MNKPDIASRFRRLVALRRVAFVAVFACLLTGGAPVTRAEGWHGL
                     TPLKSRRADVERELGKPLTTDTGSGALNFKVAGGMVTVTFVTPKFAQTKKLSPDMEGT
                     VLLIVLQHENSSDTPESVGLVNKSNFSREEKGGMAVYTNQKDGITYTFFNGRLKTTRY
                     APAADQLSGSQGLKSVL"
BASE COUNT          799 a         1298 c         1264 g          788 t
ORIGIN      
        1 gcggcgtgcg cagctctcct aaagaccatg cgcgcattct atcaacatca gctatcttgt
       61 gcgcgggcat cgaacccgcc cccaagatgg ttcatccatc cccaccgcaa gcggtggggc
      121 attaagaacc tttttcgtaa agacggtcgc acggtaattt agcgagcggg taaaaagcag
      181 agacgggagc cgcacacgcg ctggcgtggc cgctcccgtc tcctgctttc gccctcgtcg
      241 gcgtgcttcg tttacttatc ctgatcctcg tcctgcggtt tattgagccg gacgaaggct
      301 tcgccgcgcg gcgagagttc cgctttgtaa gcgaccgccg ccagtatgtt ctccagcagc
      361 tctttgttgt ccatcttgaa actcgtgacg cccgaaactc gcgtatccgg atcgctgaac
      421 tgcatggtca ggtagcgata cttcttcctg atgagcaggg cgggcagcgt gtagatgctt
      481 gcgccgccga gcacggaggc cgccgtcggc ctgcgcgatt gcgtgtcggc gtaagcggcg
      541 gcgatagact tgtaagggat cgaaatatat tccttctgcg ctttgtcgcg aaagacgagg
      601 cggcgtccct cgtcgtcgaa attgagcgtg cccgtctgtt ttttgttgta accgaagatg
      661 ccgccttcgt atttggcctt aacgctcttc ggcgcgtcta ccttcggcgc gaccggagcg
      721 ttagtgcctg tgggcggagg cagacttgcg ccacgcggcg gcggcacggg cacggcgatg
      781 gtacgcgagc gttgggcgag ggcgacgctc gccagttgag ttgacagcag caaggccgca
      841 ataagcagga gttgatattt tcttttcata aaggtctttc cttctctcca aaattttcgt
      901 ttcccttttg ttctctttag agacgcacgc cgccgagcgt gcgttgccat gcgagcgaac
      961 gtttgagcac acaaaaacgc tgccgctctt cgggcgtgca cacgtcgtcg agttgatcga
     1021 ggatggcttc gacgagcggc acggcgaacg cgcccgcgtg cgccacgatc tgcgaatagt
     1081 agtgcagccc cacgcgcccc tcgccgtctc gatgaaactc ctccatctgc gcttcatcca
     1141 gacgaaacac gtcctgcccg accggaatca cgcggcgcgg cacgctcatc tgcgtgcgcc
     1201 cgtcctcgcg ccagccgagc aggaggatgc cgatgagcac gccctgctcg ttcaaataat
     1261 cgggactcag gatggcgagg tctgccgccg gagagaggcg cggcccgaac tgcccgtact
     1321 ccgggttgac caactgcgtg ttgtaggcga tgcccaccac gtcgaagtta tccgccacgg
     1381 ggagcgagac gaattgcgcg aagccgtagt ccgaagagtc cggcggcgcg tccacgtcca
     1441 gacggtcgat cacgcgcccg acgtaatcca cgtgcgaatt tgatttaacg attttcgcga
     1501 tcttgatcat ttattcttcg ccggttgccc gctgccggtc gttcgccccg gacgcttgcc
     1561 cggcaggctc caaacagcga acggctgatg tgtggtttag aggtacgaca agatgtagag
     1621 ccagactaac acctgcgccc ccgcgatgag cagcccgccc gcgatacagg tgacgacgag
     1681 gcgcgcgccg cccatccgac ggaaaagcca cgcctcgatc aggccgaagc cgccgaacac
     1741 gatcacgaag gggctgaaca taattccgta gagggggaag agcgaacagc agaccatcac
     1801 cagcgcgatg gccgccgaag tatttccgcc gtgagggggc agactgaagc gcggcgtgcg
     1861 ctgcacccgc acgccgctca tctcctgctc gaccgacatc gccgggcggc tgtgttgttg
     1921 atgataagag gcgaggagcg aatcagccgg agcgtatgcg cgctcgcgca aaccgcgccc
     1981 gcaggtggcg cagaaatgcg cgcccgcccg ccgcgccgcc acaccgcacg cggggcaaac
     2041 tacagccgac gcctcgcggc tccggttcgc ctctccatca aacatctgct cgtgcgactg
     2101 cttcataaaa atctctgtgg cctgaacgtc gaaggttcca gaccttcaac agccgcgtct
     2161 tgaacggcga cgacgactag cggcgatgcg ctttgctgat gggtttgcgc gagacgcgaa
     2221 agcccaactt ctgctcggcg gcaaactcct gcatggcgcg caggaactgt tcgcggtctt
     2281 gcatcgtgat gaccgccgcc gcgtctgccg tctccaacgc gtaaggataa ccgttgccga
     2341 cgacgcattc ggcgcgcacc gtgtcaacca cttcgtcaac cagtcccgct tcatagaccc
     2401 acgcgggaat atcgaggcgc gcgggcgaac cctcgcccgt cgtctgtaag tagacgaaac
     2461 cgacgagcgg gttgccctct tcgtcttgaa agtcctgcat accttcgcgc acacaataac
     2521 aaaagatcgt gcggtcgccc cacgcgccga agagcgattc gccgttgccg ccgtttgtag
     2581 cagcctcggc ggcgttggtg attgagagca gccgcgcatc gaagacggtg gcggcggggc
     2641 gcgcttcctc cgagagcagg tcgagcaggc ggacgagatc gcgcgcgtaa gattgatcga
     2701 tgtagccgac gagcggcact tccgtgtcgc gcgagagttc gacggcttca acaatcgtct
     2761 gaatgtaggc gcgcggaaaa tcgagcgtct cggcgcgtgc gcgcgtggtg gagagcagga
     2821 gcgtgccgtc gaagaaggcg acgggcagac gctcgccgcg tgcgcgccag ccgcgccggc
     2881 gttcgagaaa ttccgtgagc gcgcggagtt ccaactcgaa gcggcgcagg ccgacgaggt
     2941 ctgcggcggt cgccgcgccg ccttctgcct ccaacaactc ctgcggcgtg atgatcgcaa
     3001 agcgcgactc tttacgatag ctgtcctgcc cgcccgccgt gtgcgggttc tcgaaccagg
     3061 cggcctgcac cgccgcgacg ggcagggaga tctctcgtcc cggcaggatc tggctgccgt
     3121 ctgccgcgca cgtcacgcgc tcgcgcaaca cttccaaagc ccaacgccgc gcctcttcgt
     3181 gcgaacgcca acgctccgca aaagggacga cgaaggagcg tgcgccttcg agttcggcgg
     3241 aaggaagcgc gcccggcgca cgcgccgcgc ccgccgttat cgcacgctcc gcttcgagtt
     3301 tgatctcgcc cgccgcacgc ccgccgagcg cacgcagacg ccgcgcgtac tcccccacag
     3361 acgcgttcca caccgcctcg aagtgcacaa actccgcgcg ccgctcctta agctgtgcat
     3421 tcaaatgtgg gcgatacaac ataaagagcg tgaatcgtga atcgtagatc gtgaatagag
     3481 aagaactgtc ttcttctatt tataattcac tagctacgat tcacgatttt ccttctctct
     3541 ttacagaacc gatttcagcc cttgtgagcc ggagagttgg tcggcagcgg gcgcgtagcg
     3601 tgtggtcttg agtcgcccgt tgaagaaggt gtaggtgatg ccgtccttct ggttggtata
     3661 gacggccatc cctccctttt cttcgcgcga gaagttgctc ttattcacca gaccgacgga
     3721 ctccggcgtg tccgacgaat tctcatgctg caagacgatc agcagcacgg ttccttccat
     3781 gtcgggcgag agttttttcg tttgagcaaa cttcggggtc acaaacgtca cggtcaccat
     3841 gccgcccgcg actttgaagt tgagcgcgcc gctcccggtg tcggttgtca gcggcttgcc
     3901 gagttcgcgt tccacgtcgg cgcggcgcga tttcagaggc gtgagtccgt gccatccctc
     3961 cgcgcgggtc accggcgcgc cacccgtaag cagacaagca aacacagcga cgaaagccac
     4021 ccggcgcaga gcgacgaggc ggcgaaaacg agaagcaata tcgggtttgt tcatggctgg
     4081 atgctaacgc ttgcgcggga aggtttcaag gagaaaaagt gatgcctgat gagtaaggag
     4141 aaaaagtga
//
LOCUS       DASWNS010000189         6126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_215957, whole genome shotgun sequence.
ACCESSION   DASWNS010000189 DASWNS010000000
VERSION     DASWNS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215957"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VGB76_06450"
     CDS             complement(<1..1018)
                     /locus_tag="VGB76_06450"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY0098577.1"
                     /translation="MATELKRENASLGRIEELLGTDARTLLDYTSKTIPKAQLQLPGA
                     DFVDRVWAASDRSPRVMRSLQTLFNNGRLAGTGYLSILPVDQGIEHSAGASFAPNPIY
                     FDPENIVRLAIEGGCNAVASTFGVLGVVARRYAHKIPFIVKINHNELLTYPNKFDQVM
                     FGTVDQAHEMGAVAIGATVYFGSDESTRQIMEVSEAFAYAHELGMATILWCYLRNPKF
                     KGTEGDMHTAADLTGQANHLGVTIQADIIKQKLPERNGGYNMLSMESPYGKTSKKIYE
                     ELTTDNPIDLVRYQVANCYMGRCGLINSGGESKGQTDLQEAVRTAVINKRGGGMGLIS
                     GRKAF"
     gene            complement(1206..2063)
                     /locus_tag="VGB76_06455"
     CDS             complement(1206..2063)
                     /locus_tag="VGB76_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY0098578.1"
                     /translation="MHAKIIHNGRLLEPGKSRLPALTSATLYGRGVFTTLAVHHGQPF
                     LWPEHWARLMEHAERCGVRSDQFGEATVGRALVKLIKENRVDDGRARVTLLTRAVRGV
                     WKERAGGGEVETDLLIMTGDARPAPEEGLALTLSPYRLNTLSPLAGVKSVNYLEHVIG
                     WEEAHARDFHEAVVLNERGEIASATMANIFWVTRGTIHTPALATGALAGTTRARVIAL
                     AEELAFPLVEGTYDTSHLSDADEIFLTSASHGVGLVTTYDFHRYTVAAGSVALRLREA
                     FRQYVLNAG"
     gene            complement(2168..3688)
                     /gene="pabB"
                     /locus_tag="VGB76_06460"
     CDS             complement(2168..3688)
                     /gene="pabB"
                     /locus_tag="VGB76_06460"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY0098579.1"
                     /translation="MNNAQRNGESDPSASPFVRAYEISAGELLSALLGLAPERRVQIL
                     DSGGARGDGEEARFLIAGFDPFETIEARSDELTIQARGERVARVECGSLLTLLDERLA
                     RYRVRGFAHTANRLPVAGGACIASFSYDLARQFARLRSSAPHAPHAPEPDAVLAFYDT
                     LVIHDYAHGRTYLTSLGGDAQLARTRDVLEEARRVKRFDASARDAVETQQRMAVAASN
                     FTRDGYMSAVRRIKEHIYAGDIYQANLTQQFSCALEEEDGSRSAERVFLRLRREHPAS
                     FAAFLRRREDTVVSASPERFLRVSLNEGNEADAGSDNSRRVEAWPIKGTRARGANATE
                     DARLKRELQRSAKDAAENVMIVDLLRNDLGRVCRYGSVEVAALCELQEHPTLFHLVSK
                     VRGTLRAEVSAGELLRATFPCGSITGAPKLRAMEIIDEYETAPRGLSMGAIGYFSFDG
                     HIDLSVAIRTMTLGAKQARFNVGGGIVAESDPAAEYEESLTKARALLRALGARASG"
     gene            complement(3693..6083)
                     /gene="purL"
                     /locus_tag="VGB76_06465"
     CDS             complement(3693..6083)
                     /gene="purL"
                     /locus_tag="VGB76_06465"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY0098580.1"
                     /translation="MEIITPEVIAAHNLTGEEYARIVELMGRELSFVELGIFSVMWSE
                     HCSYKSSRMHLKRLPTRNERVVVPPGENAGVVDVGDGWCAAFKVESHNHPSFIEPFQG
                     AATGVGGILRDIFTMGARPIAAMNSIRFGTLEHEHYGRRNRALLAGVVAGIAHYGNAF
                     GVPTIGGEVAFDEAYALNPLVNAFALGLVRHDQIFFGRAVGVGNPVLYVGAKTGRDGI
                     HGATMASAEFDDEALEKRPTVQVGDPFLEKLLLEACLEAMRSGAVAGIQDMGAAGLTS
                     SSCEMAARAGTGLEIDLTLVPQREQGMSAYEIMLSESQERMLIVAHKGRERQVMDIFK
                     HWELDAVVIGRVTDTGHVQIMHHEGTVADIPVGYLTDEAPVYERPMRAPATKEESAAK
                     HRWSTSVDIDGDDIKTQAPTLGETIESSEELDSVLSATKASVSAFDEDHNESLRRLLA
                     APNLASKEWVYRQYDHMVRTNTAILPGADAAIVRIKETRRALAMCLDGNGRYSAADPR
                     EGARQIVAEAARNVVCVGAKPIAVTNCLNFPSPERPEVMWAFSETIDGMAEACLAFDT
                     PVISGNVSFYNETEGRGILPTPVIGMVGLVEDVRRIIQPGFKREGDLIALLGLTQDDL
                     TISEYAATIKEVATEEMLRTGRLASIDLKLEQAVQSTCLRAAEETLLRSAHDCADGGL
                     GVALAESCFSTLNRASIGAQVELSGALSPAAELFAETPSRIIVSFDETARARLEEIAA
                     EFDCPLAVIGRVGGQDLRITIDTQPAVSQNVNELETHWRTALGKKLQAEALVVAAE"
BASE COUNT         1060 a         1880 c         2000 g         1186 t
ORIGIN      
        1 ggaaggcttt ccgtccggag atgagtccca tgccgccgcc gcgtttgttg atgacggcgg
       61 tgcgcacagc ttcctgcaaa tcggtctgcc ccttcgactc gccgcccgaa ttgatcaagc
      121 cgcaacgacc catgtagcag ttggcgacct gataacgcac gaggtcaatc gggttgtcgg
      181 tcgtcaactc ctcgtagatc ttcttgctcg tcttgccgta gggcgactcc atgctcagca
      241 tgttgtagcc gccgttacgt tccggcaact tctgcttgat gatgtcggcc tgaatcgtga
      301 cgccgagatg attggcctgt cccgtcaaat ccgccgccgt gtgcatgtcg ccttccgtgc
      361 ctttaaactt cgggttgcgc agatagcacc agaggatcgt cgccatgccg agttcgtgcg
      421 cgtaggcgaa ggcttcgctc acctccatga tctggcgcgt cgattcgtcg gagccgaagt
      481 agacggtcgc gccgatagcg accgcgccca tctcgtgcgc ctggtcaacc gtgccgaaca
      541 tcacttgatc gaatttgttc gggtaggtca aaagttcgtt atggttgatc ttgacgatga
      601 agggaatctt gtgtgcgtag cgacgcgcca ccacgccgag cacgccgaag gttgaagcga
      661 cggcgttgca gccgccttcg atggcgaggc gcacgatgtt ttccgggtcg aagtagatcg
      721 ggtttggcgc gaaactggct ccggcggaat gttcgatgcc ctgatcgaca gggaggatcg
      781 agagatagcc cgtgcccgcg agccgcccgt tgttgaagag cgtttgcagc gagcgcataa
      841 cgcgcgggct gcggtcggac gccgcccaga cgcggtctac gaagtctgcg ccgggcagtt
      901 gcagttgagc tttggggatg gtcttcgagg tgtagtcgag cagcgtgcgt gcatccgtgc
      961 cgagcagttc ttcgattctc ccaagtgacg cattctcgcg tttgagttcg gtcgccataa
     1021 actccaatcc tcctcttgtt aaataacaag cgcgttaaaa tctgccagag taagttacgt
     1081 gtacggggtc acaaggcgaa aattatagca caggaaaggc gggcgtttgc gctccgcacc
     1141 cgtgcgcctg agacgagcct gaaattagga tgtgcgaccg gacggcgggc gacgcgcgga
     1201 atttttcaac cggcgttcag cacgtattga cgaaacgcct cgcgcagacg cagggcgacg
     1261 cttccggcgg cgaccgtgta acgatgaaag tcgtaggtgg tgacgaggcc gacgccgtgt
     1321 gaggccgaag tgagaaaaat ttcgtcggcg tcgctcaagt gggaagtgtc gtaggtgcct
     1381 tcgacgaggg ggaaggcgag ttcttccgcg agggcgatga cgcgtgcgcg ggtggtgccc
     1441 gcgagcgcgc cggtggcgag ggcgggcgtg tggatcgtgc cgcgcgtcac ccagaaaatg
     1501 ttcgccatcg tcgccgaggc gatctcgccg cgctcgttca ggacgacggc ttcgtgaaaa
     1561 tcgcgcgcgt gcgcctcttc ccagccgatg acgtgttcga gatagttgac agacttgacg
     1621 cccgccagcg gcgagagcgt gttgaggcga taaggggaga gcgtcagggc gagtccctct
     1681 tcgggcgcgg ggcgcgcatc gcccgtcatg attaacaggt cggtttccac ctcgccgccg
     1741 cccgcgcgct ctttccacac cccgcgcacg gcgcgcgtca gcaaggtaac gcgtgcgcgc
     1801 ccgtcatcca ccctgttctc tttgatcaac ttgacgagcg cgcgcccgac cgtcgcctct
     1861 ccgaattggt cggagcgaac cccgcaccgc tcggcgtgct ccatgagacg cgcccagtgc
     1921 tccggccaga gaaaaggctg cccgtgatgg acggcgagcg tcgtaaaaac gccgcgcccg
     1981 tagagcgtcg cggaagtgag cgcgggcagg cgcgactttc cgggttcgag caggcgtccg
     2041 ttatgaataa tttttgcgtg catgtttcgc tcaccgaaaa acgctgatct gagttgaaac
     2101 gcgacgggcg agtggttcac cgcgaggata catgaaaagg tgagcggcgt tctaattttt
     2161 acgagggcta accgctcgca cgcgcaccga gcgcacggag cagggcgcgg gctttggtga
     2221 gcgactcctc atattccgcc gccgggtcgc tctcggccac gatgccgccg ccgacattga
     2281 agcgcgcttg tttcgcgccg agcgtcatcg tgcggatcgc aacgctcaga tcgatgtggc
     2341 cgtcgaaaga gaagtagccg atagctccca tcgagagtcc gcgcggggcg gtttcatact
     2401 cgtctatgat ctccatcgcg cgcagtttcg gcgcacccgt gatcgagccg caggggaaag
     2461 tcgcgcggag cagttcgccc gcagacacct ctgcgcgcag cgtgccgcgc actttcgaga
     2521 cgagatgaaa gagcgtcggg tgctcttgca gttcgcagag cgcggcgacc tcaaccgacc
     2581 cgtagcgaca gacgcgcccc agatcgttgc gcaacagatc gacgatcatc acgttctccg
     2641 ctgcgtcctt cgcgcttcgc tgcaactcgc gcttgagccg cgcgtcctcc gtcgcgttcg
     2701 cgccgcgcgc gcgcgtcccc ttgatcggcc acgcttccac gcggcgcgag ttgtcgctcc
     2761 ctgcgtctgc ctcgttccct tcgttcaaag agacgcgcag gaagcgttcg ggcgacgcac
     2821 tgacgacggt gtcttcgcgg cggcgcagga aggccgcgaa agatgccgga tgctcgcgcc
     2881 tgagccggag gaagacgcgc tccgcgctac ggctgccgtc ctcttcttcc aacgcgcagc
     2941 taaactgttg cgtgagattc gcctggtaga tgtcgcccgc gtagatgtgt tccttgatgc
     3001 gacggacggc ggacatgtac ccgtcgcgcg taaaattcga cgccgcaacg gccatccgtt
     3061 gctgtgtctc tacagcgtct cgcgcagacg cgtcgaagcg tttaactcga cgcgcttctt
     3121 cgagcacatc tctggtgcgt gcgagttgag catcaccgcc gaggctcgtg aggtaggtgc
     3181 gaccgtgcgc gtagtcgtgt atgacgagcg tgtcgtagaa ggcgagcacg gcatcgggtt
     3241 caggcgcgtg gggcgcgtgg ggcgcgctgc ttcgcagacg cgcgaactgc cgcgcgagat
     3301 catacgagaa acttgcgatg cacgcccctc cggcgacggg caagcggttc gccgtgtgcg
     3361 caaagcctcg cacgcgataa cgcgcgagcc tttcgtcaag gagcgtcaac agcgaaccgc
     3421 attcgacgcg cgccacgcgc tcgccgcgcg cttgaatcgt caactcatca ctgcgcgctt
     3481 caatcgtctc gaacggatcg aaacccgcga tcaggaaacg cgcttcctcc ccgtctccac
     3541 gcgccccgcc gctatcgaga atctgcaccc gccgctccgg cgcaagtccg agcaacgcgc
     3601 ttaagagttc accggcggaa atttcgtatg cgcgcacgaa cggcgaggct gatggatcgc
     3661 tctcgccgtt gcgttgtgcg ttgttcacgt ttttattcag ccgccacgac caacgcttcg
     3721 gcttgcagtt ttttaccgag tgcggtgcgc cagtgcgttt ccagttcgtt gacgttctgc
     3781 gagacggcgg gttgcgtgtc aatcgtgatg cgcaggtctt ggccgccgac gcgcccgatg
     3841 acggcaagcg ggcagtcgaa ctccgccgcg atctcttcga ggcgtgcgcg cgccgtttcg
     3901 tcgaaactga cgatgatgcg cgagggcgtt tcggcgaaaa gctctgcggc gggagagagc
     3961 gcgcctgaga gttcgacctg cgcgccgatg gaggcgcggt tgagcgtgga gaagcagctt
     4021 tcggcgaggg cgacgccgag gccgccgtcc gcacagtcgt gcgcggaacg gagcagcgtc
     4081 tcttccgccg cacgcaggca ggtggattgc acggcctgtt cgagtttgag atcgatgctg
     4141 gcgaggcgtc ccgtgcgaag catctcttcg gtggcgactt ctttgatcgt cgcggcgtac
     4201 tcgctgatgg tcaggtcgtc ttgcgtgagg ccgaggagcg cgatgaggtc gccttcgcgt
     4261 ttgaaaccgg gttggatgat gcggcgcacg tcttccacga gtccgaccat gccgatgacg
     4321 ggcgtgggca ggatgccgcg cccctccgtc tcgttgtaga aggagacgtt gccggagatg
     4381 acgggcgtgt cgaaggcgag gcaggcttcg gccatgccgt caatcgtctc ggagaacgcc
     4441 cacatcactt cggggcgttc gggtgagggg aagttcagac agttggtgac ggcaatcggc
     4501 ttcgcgccga cgcagacgac gttgcgcgcg gcttcggcga cgatctggcg tgcgccttcg
     4561 cgcgggtcgg ctgccgagta gcgaccgttg ccgtcgagac acatggcgag cgcgcggcgc
     4621 gtctccttga tgcgcacgat ggccgcgtcc gcgccgggca ggatcgccgt gttcgtacgc
     4681 accatgtggt cgtactggcg atagacccac tccttcgagg cgaggttggg cgcggcgagc
     4741 agacgccgga gcgactcgtt gtgatcttcg tcgaacgcag agacgctcgc cttcgtcgcg
     4801 gagagaaccg agtcgagttc ctccgagctt tcaatcgtct cgccgagcgt cggagcctgc
     4861 gtcttgatgt cgtcgccgtc gatgtctacg gaggttgacc agcggtgttt cgccgcgctc
     4921 tcctctttcg tcgcgggcgc gcgcatcggg cgttcgtaga cgggcgcttc gtccgtgaga
     4981 tagccgacgg ggatgtccgc cacagtgcct tcgtggtgca tgatctggac gtgcccggtg
     5041 tccgtcacgc gaccgatgac gacggcgtcg agttcccagt gcttgaagat gtccatcacc
     5101 tgccgctcgc gccccttgtg cgcgacgatg agcatgcgct cctgcgattc cgagagcatg
     5161 atctcgtagg cggacatgcc ctgttcgcgt tgcggcacga gcgtcagatc aatttcgagt
     5221 ccggttccgg cgcgcgccgc catctcgcac gaagaggagg tcagacccgc cgcacccatg
     5281 tcctgaatgc ccgccaccgc gccgctgcgc atcgcttcga ggcacgcttc gagtagcagt
     5341 ttttcgagaa acggatcgcc gacctgcacg gtcgggcgtt tctccagcgc ctcgtcgtca
     5401 aactctgcgg aggccatcgt cgcgccgtga atgccgtcgc gccccgtctt cgcgccgacg
     5461 tagagcaccg gattgccgac tccgaccgcg cgcccgaaga agatttgatc gtggcggacg
     5521 aggccgagcg cgaacgcgtt gaccagcggg ttcagagcat acgcttcatc gaaagcgact
     5581 tcgccgccga tggtgggcac gccgaacgcg ttgccgtagt gtgcgatgcc tgcgaccacc
     5641 ccggcgagga gcgcgcgatt gcgccgcccg tagtgttcat gttcgagcgt gccgaagcgg
     5701 atggagttca tcgccgcgat ggggcgcgcg cccatcgtga agatgtcgcg caggatgccg
     5761 cccacgcccg tcgccgcgcc ctgaaacggt tcgatgaacg aaggatgatt gtgcgactcg
     5821 accttgaagg ccgcgcacca gccgtcgccc acgtccacga cgcccgcgtt ctcgcccggc
     5881 ggcacgacga cgcgctcgtt ccgcgtcggc agacgcttca ggtgcatgcg cgaagattta
     5941 taagaacagt gttccgacca catcaccgag aagatgccga gttccacaaa cgacaactcg
     6001 cgccccatca actccacgat gcgcgcatac tcttccccgg tcagattgtg tgcggcgata
     6061 acttccggtg tgatgatttc cataagttaa atgagtgatg agtaatgagt gatgagtgat
     6121 gagtga
//
LOCUS       DASWNS010000190         8043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_59164, whole genome shotgun sequence.
ACCESSION   DASWNS010000190 DASWNS010000000
VERSION     DASWNS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8043
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59164"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VGB76_06470"
     CDS             <1..845
                     /locus_tag="VGB76_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0098581.1"
                     /translation="NAEGKRFMPRYHERAELAPRDIVSRAIVAELERTATRTAFIDMT
                     ALDGRYLRRRFPKIYDTCLRYGLDMTRELIPVSPAAHYIMGGVRTDLDGRTTVAGLYA
                     AGEVACTGVHGANRLASNSLLEGLVFGARAGRAATREHASDSHVARRAAIEERDAESA
                     AVSDRDAGEIALAVRKRVRRLMWERVGILRSRQSLTRALGEFEQIERARSLRAPSRNF
                     VTVALLVARAALWREESRGAHFRYDFPERDDARWRVHSIARRGAPVRASETVDFSPAD
                     KAVV"
     gene            1244..2275
                     /locus_tag="VGB76_06475"
     CDS             1244..2275
                     /locus_tag="VGB76_06475"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEY0098582.1"
                     /translation="MVDKNETLTLTVDASVAGTPVLNERLKYYLDKPVRELIALDAKP
                     LSPGQKERHRLYSLTLMTLVHHYWNGNKYGRDGEYPWNEPADAEDGKYLNRDYLGHNI
                     AAIAVDGDGRVIDFDFNHNKIFNSSVEHAEARLVRRVYGLAQVQDTWNMNDFSLHSPA
                     KDDYNTLENVTVYTSLESCSQCAGIMALGRVKEIVYLQSDPDMYMIGNILHNLTLDTK
                     LQAPLPIAASEDSLSYFNRLNSAFVRFTRQVAEKPFFIPKGGKPDHSKSITSFLCTKA
                     ARDIYGEAAAAFAEYLNDENKLEHPTFKPLDKDGQPVKTALTNRQSLAELKCFLSYAV
                     GNGRRGTPH"
     gene            2444..3175
                     /gene="nfi"
                     /locus_tag="VGB76_06480"
     CDS             2444..3175
                     /gene="nfi"
                     /locus_tag="VGB76_06480"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HEY0098583.1"
                     /translation="MRRRNFVEPGHTMAHYEQLHDWEMTPREAVELQKRMRGQVRVEP
                     HRGGIETVAGADISFNKFSPVIYTGIVVLRLPSLEVVEEVGVVGETQFPYVPGLLSFR
                     EAPSVLEAWAKLKTEPDAVMFDGQGIAHPRRVGIASHVGLFLNRPTFGCAKSILVGRH
                     DELAEERGSWQPLVDPKNDETVGAALRTKTRVQPIYVSPGHLIDLAGAIELTLRCDGG
                     YRQPEPTRRAHLLVNALRRGEREAT"
     gene            complement(3296..4924)
                     /locus_tag="VGB76_06485"
     CDS             complement(3296..4924)
                     /locus_tag="VGB76_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEY0098584.1"
                     /translation="MARSISLRSTLSLVLIALFALAAPVAFAQKDKKKSSDTQDVMER
                     PRKQKEELKKIYKEWVDKDVAYIITGAERSAFKKLVTDEEREQFIEAFWQRRDPDPDT
                     DENEYKEQYYERIAYANEHYASGIPGWKTDRGRIYITFGKPDSVESHPTGGSYDRPSY
                     HGGGSTTTYPFEVWFYRHIEGIGSGIEIEFVDPTGTGEYRIARSPDEKDALAMVPGAG
                     LTLNESLGLSNKSDRLAYGNDYQRQQDSPFARLQLIADLSRPPAIKNAELASLLTGSG
                     TIEDNPLNFDMRIDFFRQSDERVVTAFTIQTENKELTFKDIGGVQTARMNIFGRITSV
                     AGRRVGIFEDAVTTTATSDELLGAKSRKSAYGKTVPLPPGTYRVDVIVRDVESGATQV
                     KHLGFTVPKYDAKQLSTSTLVLAAKLQNTSDQPAVGQFTIGQFKVIPNVARSYRKGDP
                     IGIYMQVYNAGIDQTTLRPSVDVDYVLLKDGKELGKMAEDWRGMSDAGQRLTLARLIM
                     SDQLTPGEYELMIRIKDRVSGQSLSPSEKFSITQ"
     gene            complement(5312..6391)
                     /gene="tatC"
                     /locus_tag="VGB76_06490"
     CDS             complement(5312..6391)
                     /gene="tatC"
                     /locus_tag="VGB76_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY0098585.1"
                     /translation="MASTLLEGDTNSDEQDSRLGGQMSFLDHLDELRRRLVRSIVFVF
                     IAFMGCWFISDYIYNFLARPVRFALADAQRRQLPLGGLTGKEATRALSSAQENDTGRY
                     VFADTTNLGESVIPVGTSVAARVARDAQGQLGLFTDEPIYAGGTIIPKGVRLPFDLNA
                     QPDALTGIDDRLVVNTALEPFSLYVKVSLYAALCLSVPFLLWQIWAFVAPGLYPHERG
                     YALPFVVLSSLFFVGGAAFAYYIIFPPAARYLLGLGQDFRLLLKADDYFDFIIFIMLG
                     MGLVFQMPAVTYILSRIGIVTARLLLRIWKTSLIVILVAAAIISPTSDVPNMMLFAAP
                     MLVLYIISIFVAWIFGKPRTTEAGA"
     gene            complement(6453..6884)
                     /locus_tag="VGB76_06495"
     CDS             complement(6453..6884)
                     /locus_tag="VGB76_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY0098586.1"
                     /translation="MVLLFLESLGTTELLVILLVALVLFGPRKLPEMTRKVAKSLNEF
                     KRASDDFQRTWEREVALESVQREAAIDRALVAEPQPGEFAAEANIAGEPKPARDITEA
                     AVAPVQGANIARGSHVEMPAPVNAGQTETAEQQPSSKTDWL"
     gene            complement(6996..7883)
                     /locus_tag="VGB76_06500"
     CDS             complement(6996..7883)
                     /locus_tag="VGB76_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098587.1"
                     /translation="MKFSKSGSRLGALAFALASLTITVQGQIGETPTAQPPQIRTTPS
                     IFDPTRPEPDKTMSGMSGARSSRSGTYRPPPRRPMTEEHRRRLSPSAEERARFATFLR
                     QPQSGLVRLLPQSYCGTDPRVLEVGNACLDAIPPVPGGGSFYSFWSSEHESARLSDVW
                     LKDGMFRAGVAGEALGVLTALGDVPLEYVTLQTPGVAYLAQFVPPTTIADAQRQHAQS
                     NIGFSVTGRAYGSAAQVRPDATYVLRSIIYRSDSSLNKTRKPSDVLVSFRVVSKAEDG
                     SVTLLWRELARSKPPKLKK"
BASE COUNT         1542 a         2429 c         2515 g         1557 t
ORIGIN      
        1 gcaacgccga aggcaaacgc ttcatgccgc gctatcacga acgcgccgaa ctcgcgccgc
       61 gcgacatcgt cagccgcgcc atcgtcgccg aactggaacg caccgccacg cgcaccgcct
      121 tcatcgacat gaccgcgctc gacgggcgtt acctgcgtcg tcgcttcccc aaaatctacg
      181 acacctgttt gcgctacggt ctggacatga cgcgcgaact catccccgtc tcgcccgccg
      241 cgcattacat catgggcggc gtgcggacag acctcgacgg gcgcactacc gtcgccggac
      301 tctacgcggc gggcgaagtg gcctgcacgg gcgtccacgg cgcaaaccgt ctcgcgtcga
      361 attcgctgct cgaaggtctg gtcttcggcg cgcgcgccgg acgcgccgcc accagagaac
      421 acgcgagcga ctcgcacgtt gcgcggcgcg ccgctattga agagagagac gcggagagcg
      481 cggcggtgtc agacagagac gcgggagaga tcgcgctcgc cgtgcgcaag cgcgtgcgcc
      541 gcctgatgtg ggagcgcgtc gggatacttc gctcgcggca atctctgacg cgcgcgctcg
      601 gagagtttga acagatcgag cgtgcgcgca gcctccgtgc gccctcacgc aacttcgtga
      661 cggtcgccct gctcgtcgca cgcgccgcgc tctggcgcga agaatcacgc ggcgcacact
      721 tccgctacga cttccccgaa cgcgacgacg cgcgctggcg cgtccattca atcgcgcgca
      781 gaggcgcgcc cgtccgcgcg agcgagacgg tcgatttttc accagccgac aaagctgtgg
      841 tgtgaatcgc cacgaatctc gcccgggcaa taacgtagtc ggctcacccg tctccgcgtc
      901 gtcgggtctt gctccggcga aggtgtccgt ccctgcgccg atcttcttag ttttaaaatc
      961 gcctccagta tcaacacgcg gcgggcaacg tcattgacat aaatcatggg ttgtaataaa
     1021 aggcttattg ttttacggca gcactttgtt cccttgaaac ggcattacgc cttcttcacc
     1081 tgcgccctca gttcgacact gaatgcccgg aagttgtatt taacctacgc tatcgagtat
     1141 gtgaacagtc cccgcgtggc cgtgacgccg tcggatcgta ccggctgagg aagcccgacc
     1201 gtttagcgct aaccactcag gcacgagttt cggaaggatt gacatggttg acaagaacga
     1261 aacattaacc ctcacggtag acgcctccgt tgctggaacg cccgtcctca acgagcgcct
     1321 gaagtattac ttagacaagc ccgtgagaga gctgatcgcc ctcgacgcca aacccctgtc
     1381 gcccggccag aaggaacgcc accgcctcta ctcgctgacc ctgatgaccc tcgtgcatca
     1441 ttactggaac ggcaacaagt acgggcgcga cggcgagtac ccgtggaacg aacccgccga
     1501 cgccgaggac ggcaagtatc tcaaccgcga ctatctgggg cacaacatcg cagccatcgc
     1561 cgttgacggt gacggtcgcg tcatcgattt cgacttcaac cacaataaaa tcttcaacag
     1621 ctcggtcgag cacgccgaag cgcgtctggt gcgccgtgtt tacgggctgg cgcaagtcca
     1681 ggacacttgg aacatgaatg acttctccct gcactcgcct gccaaggacg actacaacac
     1741 gctggagaat gtcacggtat atacctcttt ggagtcttgc tcgcaatgcg ccgggatcat
     1801 ggcgctcggt cgcgtgaagg agatcgttta cttacaatcc gatcccgaca tgtatatgat
     1861 cggcaacatc ctgcataacc tgacgctgga cactaaactt caagcgccgc tcccgattgc
     1921 cgcgagcgag gacagcctct catatttcaa ccggctgaac agcgcattcg tcaggttcac
     1981 caggcaggtc gccgagaagc cgtttttcat ccccaagggc gggaaacccg accactcgaa
     2041 gtctattacc tctttcctgt gtaccaaggc agcccgcgac atctacgggg aggcagccgc
     2101 cgcattcgcc gaatacctga atgacgagaa taaacttgag caccctacgt ttaaaccctt
     2161 agacaaggac gggcagccgg tgaagaccgc cctgacgaac cggcagagcc tcgccgaact
     2221 gaagtgcttc ctttcatacg ccgtcggcaa cgggaggcgc gggacacctc actgatcgag
     2281 gggtttcgcc ggtagcgtcg cgcagttcgc gcggtcgtct tgtgcggatg cccgtccacg
     2341 cgcgggacgg cactgcgatc tcccccttgt cgaaaacaaa attccggcgg cgtttcgatc
     2401 ccgccgtgcg gtgggtagcc ggggcgcgca gcctgtcggc tgaatgcggc ggcgaaactt
     2461 cgtcgaaccc ggacacacga tggctcatta cgaacaactg cacgactggg agatgacgcc
     2521 gcgcgaggcg gtggagttgc agaagcgcat gcgcgggcag gtgcgcgtcg agccgcaccg
     2581 gggggggata gagacggtgg cgggcgcgga catctcgttc aataaattct cgcccgtcat
     2641 ctacacgggg atcgtggtgc ttcgtctgcc gtcgctcgaa gtggtggagg aagtaggcgt
     2701 ggtgggcgag acgcagtttc cctacgtgcc ggggctgctc tcgtttcgcg aagcgccgtc
     2761 ggtgttggag gcgtgggcga aactcaagac ggagccggac gcagtgatgt tcgacgggca
     2821 gggcatcgca cacccgcgcc gcgtcggcat cgcctcgcac gtcggtttgt ttctgaaccg
     2881 accgacgttc ggctgcgcca agtctattct cgtcggacga cacgacgagt tggccgagga
     2941 gcgcggcagt tggcagccgc tcgttgaccc gaagaacgac gagacggtcg gcgcggcctt
     3001 gcgtacgaag acgcgcgtgc agccgatcta cgtctcgccc ggccacctca tcgatctcgc
     3061 gggcgccatc gaactcacgc ttcgctgcga cggcggctat cgccaacccg aacccacgcg
     3121 ccgcgcgcat ctcctcgtca acgccctccg gcgcggggag cgggaagcca cttagaaccc
     3181 atgcggcgac aaatccgcgt tccggccttc acttaaccaa aattaacccg cgcataaaaa
     3241 gagcggcggc aagtctgaga cttgccgccg ctctttttat gtctgcgtct cgccgttatt
     3301 gagtgatcga aaacttctcg gagggcgaga gcgattgacc ggagacgcgg tctttgatgc
     3361 gaatcatcaa ttcatattcg ccgggggtaa gttggtcgct catgatcagg cgggcgagcg
     3421 tgagacgctg cccggcgtcg ctcatgccgc gccagtcttc cgccatcttg ccgagttcct
     3481 tgccgtcttt caagagcacg tagtccacgt caaccgaagg gcggagcgtg gtctggtcaa
     3541 tccccgcgtt gtagacctgc atgtagatgc cgatggggtc gcccttgcga tagctgcgcg
     3601 cgacgttcgg gatcactttg aactggccga tggtgaactg cccgaccgca ggctgatccg
     3661 aagtgttctg caacttggcc gcgagcacga gcgtggaggt cgagagttgc ttggcgtcgt
     3721 acttcggcac ggtgaagccg agatgcttca cctgcgtcgc gccggattcc acatcgcgca
     3781 cgataacgtc cacgcgatac gtgcccggcg gaagcgggac ggtcttgccg taggccgact
     3841 tgcggctctt cgcgccgagc aactcgtcgc tcgtagccgt cgtcgtgacg gcgtcttcga
     3901 agatgccgac gcggcgaccc gcgaccgaag tgatgcggcc aaagatgttc atgcgcgccg
     3961 tctgcacgcc gccgatgtct ttgaaggtca actccttgtt ttccgtctga atggtgaagg
     4021 ccgtgacgac gcgctcgtcc gactggcgga agaaatctat ccgcatgtcg aagttgagcg
     4081 ggttgtcttc aatcgtgccc gatccggtca ggagcgaggc gagttcggcg ttcttgatgg
     4141 cgggcgggcg cgaaaggtct gcgatgagtt gcaggcgcgc gaaggggctg tcctgctgtc
     4201 gctggtagtc gttgccgtag gcgaggcggt cggatttgtt ggagaggccg aggctctcgt
     4261 tcaaggtcag gccagcgccg ggaaccatgg cgagcgcgtc cttctcgtcg ggcgagcgcg
     4321 cgatgcgata ttcgcccgtg ccggtcgggt cgacgaactc gatctcgatg ccgctgccga
     4381 tgccttcgat gtggcggtag aaccagactt caaaggggta ggtcgtcgtg gagccgccgc
     4441 cgtgatagct cggcctgtca taggagccgc ccgtcgggtg cgactcgacg gaatcgggct
     4501 tgccgaaggt gatgtagatg cgaccgcgat ccgtcttcca gccggggatg cccgaagcgt
     4561 agtgctcgtt ggcgtaagcg atgcgctcgt aatactgttc cttgtattcg ttttcgtccg
     4621 tgtccgggtc tggatcacga cgctgccaga aggcttcgat gaactgttcg cgctcttcat
     4681 ccgtgacaag cttcttgaac gcggaacgct ctgcgcccgt gatgatgtag gccacgtcct
     4741 tgtccaccca ctccttgtaa atcttcttga gttcttcctt ctgtttgcgc gggcgttcca
     4801 tcacatcctg cgtgtcggaa cttttcttct tatctttttg cgcaaaggcg acgggcgcgg
     4861 cgagcgcgaa gagggcgatg agaacgagac tgagcgtgct gcgtaagcta atggaacgag
     4921 ccatcgttgt actactcctg ttgcgtattc aaacgtaaac tttaggttcg ctggtgtccg
     4981 cgcctgtctg cgtttcgcgc ggcgcgtgcg agtgtgaaag aaaacccttg ataaattcga
     5041 ctggaagtca tgaaaacggc gacgccgaaa ggtcaaaacc gctttcccaa ccgggacttg
     5101 accgctgagc atcgccaaac tttcgagccg cgattgtaga gcctcggttg gactaggtca
     5161 agatgcgccg gagcgggcaa aagttgcctc ggagcagagg ttagaggccg gcgttgagag
     5221 gttggatgcc agcgaaaatc agttttctgt ctttaataac ggcctcgata acggcttccg
     5281 gcctctgtgt ctttatagcg gccttcggcc tctaggctcc ggcctcggtt gttctcggct
     5341 tgccgaaaat ccacgcgacg aagatggaga taatgtaaag gacgagcatc ggggcggcaa
     5401 agagcatcat gttgggcaca tcgctcgtgg gcgagatgat ggccgccgcg acgaggatga
     5461 cgatgagcga ggtcttccag atgcgcagca gcagccgtgc cgtgacgatc ccgatgcgcg
     5521 agagaatgta ggtgacggcg ggcatctgga agacgagtcc catgccgagc atgatgaaga
     5581 tgatgaagtc gaaatagtcg tcggctttta agagcaggcg aaagtcctgt ccgagtccga
     5641 gcaggtagcg cgccgccggg ggaaagatga tgtagtaggc gaaggccgcg ccgccgacga
     5701 agaagagcga ggagagcacg acgaagggca gcgcgtagcc gcgttcgtgc gggtagaggc
     5761 cgggcgcgac gaaagcccag atttgccaca gcagaaacgg cactgacagg cagagcgccg
     5821 cgtagagcga aactttgacg tagagcgaaa agggttcgag cgcggtgttg acgacgaggc
     5881 ggtcgtctat gccggtgagc gcgtcgggct gcgcgttcag gtcgaagggg aggcgcacgc
     5941 ctttcgggat gatcgtgccc ccggcgtaga tcggttcgtc cgtaaagagg ccgagttgac
     6001 cctgcgcatc acgcgcgacg cgcgccgcga cagacgtgcc gaccggaatc acgctctcgc
     6061 cgaggttcgt ggtgtcggcg aagacgtaac gccccgtgtc gttctcctgc gcggaactga
     6121 gcgcgcgcgt ggcctccttt ccggtgaggc cgcctaaggg gagttggcgt cgctgcgcgt
     6181 cggcgagcgc gaagcgcacc gggcgcgcga gaaaattgta gatgtaatcg gagatgaacc
     6241 agcagcccat gaaggcgatg aagacgaaga cgatggagcg gacgaggcga cggcgcagtt
     6301 cgtccagatg atcgagaaag gacatctggc cgccgagccg tgaatcctgc tcgtcgctat
     6361 tggtgtcgcc ttctaagagt gtggatgcca tcggattcgg gcgggttaat ctcgaagagt
     6421 cttcgctggc aggtaagtct gcacgggctg gctcacagcc aatcggtctt gctcgaaggt
     6481 tgttgctccg ccgtctccgt ctggccggcg ttgacggggg cgggcatctc gacgtgtgag
     6541 ccgcgcgcga tgttcgcgcc ctgcacggga gcgacggcgg cctctgtgat gtctcgcgcg
     6601 ggcttcggct ctcccgcgat gttcgcttcc gccgcaaact cgcccggctg cggttccgca
     6661 accaacgcgc ggtcaatggc ggcttcgcgc tgcacgctct ccaaggccac ctcgcgctcc
     6721 catgtccgct ggaaatcgtc cgacgcgcgt ttgaactcat tcagactctt ggcgaccttg
     6781 cgcgtcatct ccggcagctt gcgcggcccg aacaacacga gtgcaaccaa cagaataacg
     6841 agcagttccg tcgtaccgag agattcgaga aacaggagaa ccataaagta accagttcgt
     6901 cagtagtagt cgtttatgaa gcgtcggagc ctttcaacgt cggctgccgg tcaaactctg
     6961 tccggcaatt ctattacccg cccctgacct ccgccttact ttttgagttt gggcggttta
     7021 cttctggcta gctcgcgcca gagcagcgtg acgcttccgt cttccgcctt gctcacgaca
     7081 cgaaacgaca cgagcacgtc cgagggcttg cgtgtcttat tgagtgagct atccgatctg
     7141 tatatgatcg agcgaagcac gtaagtggca tcaggccgca cttgagccgc agagccgtag
     7201 gcgcgccccg tcacgctgaa ccctatatta ctctgcgcgt gctgtcgctg cgcgtcggcg
     7261 atggtcgtgg gcggaacaaa ctgcgccaga taggccactc ccggcgtttg cagcgtgacg
     7321 tactcaagcg gcacatcgcc aagcgcggtc aatacgccga gcgcttcgcc cgccacccct
     7381 gcgcggaaca tcccatcctt gagccagaca tccgagagcc gcgccgactc gtgttcgctg
     7441 ctccagaacg agtagaaaga accgccgccg ggcacgggcg ggatggcatc caggcaagcg
     7501 ttcccgacct ccaacacgcg cgggtctgtc ccgcagtagc tctgtggcaa caggcgcacc
     7561 agcccggatt gaggttgccg gaggaaggtc gcaaacctcg cgcgctcctc cgccgaagga
     7621 gacaggcgac gcctgtgttc ctctgtcatc ggtcttcgcg gcggcggcct gtaggttccg
     7681 ctgcgagatg aacgcgcccc ggacatgccg ctcatggtct tatccggctc gggtctggtg
     7741 ggatcgaaga ttgagggcgt agttcgtatc tgcggcggtt gtgccgtggg cgtctcgcct
     7801 atctgcccct gcacggtaat tgtgagagag gccagcgcga aggccaacgc ccctaatctc
     7861 gaccctgatt tagagaactt cataaatctc tccttcatcc ggacttccac cgccgggcat
     7921 gccaactgcc gtctaagtat cctgaccgta atccggcggg taatagatca cttcgatgac
     7981 ctgcccgccg atgcctttcg tgtaaaagga gacggtgcgg tcgcggtgtt ctttgagcgg
     8041 gcg
//
LOCUS       DASWNS010000191         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_267381, whole genome shotgun sequence.
ACCESSION   DASWNS010000191 DASWNS010000000
VERSION     DASWNS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267381"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="VGB76_06505"
     CDS             <1..1704
                     /locus_tag="VGB76_06505"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY0098588.1"
                     /translation="AIAQVDGSIDYFRTVVPQMFERAGGGRLPAARRAEFEATNASVV
                     SALASFRDWLRAELLPRSNGDYAIGAENYRRKLLYEEMVETPLARLINEGERELRRTQ
                     NEMREVAEEIARGRDVLSVIRSLGREHPSGDGLVGETRAELDRIRAFVRTQNILTPPA
                     RENLQVAETPAYARSTSFASMDTPGAFERVATEAYYYVTPPDPAWDARRREEHLSFYN
                     RYSLPIVSIHEVYPGHYYQLLAARQTASRVRAVFGSASFIEGWAHYSEQMMLEEGFGG
                     NNPKLRLAQLQAALSRLCRYLVGLKMHTQGMTYEQGTEFFMREGYMERANAEREARRG
                     TLDPTYLVYTLGKMEILKLREAYRERTGASFRLGEFHDRLLAYGSPPVRILRMALLGD
                     ASSAGAGGRQTPDASTPESEDRTNVVEFSVLATGAHSRHEGGRMVELITNASEWQRAW
                     GTVGGGRPVPEVNFSSRACIVVYQGQKPSGGYSLELEEIRRDGTVLAIKVRERRPAFG
                     DVTTQVITSPFVVVSIPRPPQGASVRLADEGAGGVERETQQNRKIRGKPRGRRGANRR
                     R"
     gene            2028..3437
                     /locus_tag="VGB76_06510"
     CDS             2028..3437
                     /locus_tag="VGB76_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY0098589.1"
                     /translation="MKNINYRNQSRARGFFAWLMVLTVALMPLSALAQGTRIVAPKNG
                     YKIQDDVQAGQQAAREAEQQLPLLNDQEVQNYVERVGQRLVSAIPSEFNHPEFRYSFK
                     VVNAREINAFALPGGPMYLNRGMIEAARSEGEMAGVMAHEISHVALRHGTAQATKAQK
                     YSILGGIGAIAGAVIGGGLGNVIGQGSQTAVGVYFLKFGREYETQADILGAQIMARAG
                     YDPRDLARMFQTIQQQGSRGGPEFLSSHPNPENRYARINQEAQLLGAVRNPAGNTRDF
                     QLVQARLRGQGRAPSMSEIAQGQRQGGQNYPTARRGQVAYPSTRYRQVNSNLFSLSVP
                     DNWRDYAGQNSVTLAPEGAADQNGVTHGVILGIDQSQSRNLRQATDQYLNEVLQGNPY
                     LRAQGGYTRANVSGRAGLATTLSGRSPVTGQTEIVTVVTTMLRDGSIFYMNYVTPQYD
                     QRNYQNAFNQILRSIRLND"
BASE COUNT          694 a         1025 c         1166 g          624 t
ORIGIN      
        1 gccattgcgc aggtggacgg cagcattgat tacttccgga cggttgtgcc gcaaatgttc
       61 gagcgcgcgg gcggcggtcg gcttccggcg gcgcggcgcg cggagtttga agcgacgaac
      121 gcgagcgtcg tctccgcgct cgcttcgttc agggattggt tgcgcgcgga acttttgccg
      181 cgctcgaacg gcgactacgc catcggcgcg gagaactatc gccgcaaact tctctatgaa
      241 gagatggtgg agacgccgct ggcgcgtttg attaacgagg gcgagcgcga gttgcgccgc
      301 acgcagaacg agatgcgcga ggtggccgaa gagatcgcgc gtgggcggga tgtgttgagc
      361 gtcatacgtt ctctggggcg cgagcaccct tcgggcgacg gcctcgtcgg ggagacgcgc
      421 gccgagttgg accgcatacg cgccttcgtg cgcacgcaga atattctgac gccgcccgca
      481 cgcgaaaatt tgcaggtggc ggagacgccc gcctacgcgc gctccacgag tttcgcttcg
      541 atggacacgc cgggcgcgtt cgagcgcgtc gccacagagg cttactatta cgtgacgccg
      601 ccagaccccg cctgggacgc gcggcgcagg gaagagcacc tcagtttcta taatcgctat
      661 tcgctcccca tcgtctcgat ccacgaagtc tatcccggcc actactatca actgctcgcc
      721 gcgcgtcaaa ccgcttcgcg cgtgcgcgcc gtgtttggct ccgcgagttt catcgaaggc
      781 tgggcgcact actctgagca gatgatgttg gaagaaggtt tcggcggcaa caatccgaag
      841 ttgagactcg cgcagttgca ggccgcgctc tcgcgcctgt gccgttatct ggtcggcctc
      901 aagatgcaca cgcaggggat gacttacgaa cagggcacgg agttcttcat gcgcgagggc
      961 tacatggaac gcgccaacgc cgaacgcgag gcgcgacgtg gcacgctcga cccgacttat
     1021 ctcgtctaca cgctcggcaa gatggagatt ttaaagttgc gcgaggctta ccgcgaacgc
     1081 acgggcgcat cgtttcgcct cggagagttt cacgaccgcc tgctggcgta tggcagcccg
     1141 cccgtcagga ttttgcgtat ggccttgctc ggcgatgcga gcagcgcggg cgcgggcggc
     1201 aggcagacgc cggacgcttc cacgccggag tcggaagacc gcacgaacgt cgtcgagttt
     1261 tccgtgctgg cgacgggcgc gcacagcagg cacgaaggcg ggcggatggt cgagttgatc
     1321 acgaacgcga gcgagtggca gcgcgcgtgg ggcacggtcg gcggcgggcg gcctgtgccg
     1381 gaggttaatt tcagttcgcg tgcctgtatc gtcgtctatc agggacagaa gccgtcgggc
     1441 ggttacagcc tcgaactcga agagatcagg cgtgatggca ctgtgcttgc tataaaggtg
     1501 cgagagcggc gtccggcgtt cggagacgtt acaacacagg tcatcacctc gccctttgtc
     1561 gtcgtttcga ttccgcgacc gccgcagggc gcgagcgtcc ggctggcgga cgagggcgcg
     1621 ggcggggttg agcgggaaac gcaacaaaat cgcaagattc ggggcaaacc gcgcgggcgg
     1681 cgcggcgcga atcgcaggcg ttagccggag ctaaaagagg ccaacctgcg agtggccgag
     1741 ttcatccaag cagagccgga ataatacacg gcgcgcaagt tagcgcggac gctttttccg
     1801 gtatttaaga atcaacgctc tcccgttgca gaaaccggcg caagtgtagt ttggcgtccg
     1861 gtgagtcgaa caggatttca gggggagatg cttttcgtag aagagcggcg cgaacccctc
     1921 gtccagagtc gagaggaaac gagcgtcgct cttttagttt gcatctgtaa agattagaac
     1981 gcgaacgtcc ggcaaccgaa gttcgcgtct ttatcgaagg agagagcatg aaaaatatta
     2041 actaccgtaa tcaaagccgc gcacgtggtt tttttgcgtg gctgatggtc ttgaccgtcg
     2101 ccttgatgcc gctgtccgcg ctcgcgcagg gcacgcgcat cgtcgcgccg aagaacggtt
     2161 ataaaattca ggacgacgtg caggccggac aacaggccgc gcgcgaggcc gagcagcagt
     2221 tgccgctctt gaacgaccaa gaggtgcaga actacgtgga gcgcgtcggc caacgcctcg
     2281 tcagcgcgat tccgagtgag tttaatcacc ctgagtttcg ctacagtttc aaagtcgtca
     2341 atgcgcgcga gatcaacgcg ttcgctctgc cgggtggccc gatgtatctc aatcgaggga
     2401 tgatcgaggc ggcgagatcg gaaggcgaga tggcgggcgt gatggcgcac gagatcagcc
     2461 acgtcgcgct gcgtcacggc acggcgcagg ccacgaaggc gcagaagtat tctatcctcg
     2521 gcggcatcgg cgcgattgcg ggcgcggtga tcggcggcgg cctcggcaac gtgatcggcc
     2581 agggttcgca gacggcggtg ggcgtctatt ttctgaagtt cgggcgcgag tatgagacgc
     2641 aggccgacat cctcggcgcg cagatcatgg cgcgcgccgg ttacgacccg cgcgatctgg
     2701 cgcggatgtt tcagaccatt caacagcaag gctcgcgcgg cggtcctgaa tttttgagca
     2761 gccacccgaa ccccgaaaat cgttacgcgc gcatcaatca ggaagcgcaa ctgctcggcg
     2821 cggtgcgcaa ccccgctggc aacacgcgcg atttccaact cgtgcaggcg cgtctgcgcg
     2881 ggcagggacg cgcgccttcg atgagcgaga tcgcacaggg tcaaagacag ggcgggcaga
     2941 actatccaac cgcgcgtcgc ggtcaggtcg cctatccttc gacgcgttat cgtcaggtca
     3001 acagtaacct gttcagcctg agcgtgccgg acaactggcg cgactatgcc gggcaaaact
     3061 ccgtgacgct cgcacccgaa ggcgcggctg accaaaacgg cgtgacgcac ggcgtgatcc
     3121 tcggcatcga tcaatcgcag agcaggaatt tgcgacaggc gacggatcaa tatctcaacg
     3181 aggtcttgca gggtaacccg tacctgcgcg cgcagggcgg ttatacacgc gcgaacgtct
     3241 ccggtcgcgc cgggctggcg accacgctct cagggcgttc gcccgtcacc gggcagaccg
     3301 agatcgtgac ggtcgtgacg accatgctgc gcgacggcag catcttctac atgaactacg
     3361 tgacgccgca gtatgaccag cgcaactacc agaatgcgtt caatcaaata ctgcgcagca
     3421 ttcgtctgaa cgactaaaag agttcgggcg ggttcgcagt cggtgggtta aaaagcggaa
     3481 ggccggttcg ttcgaacgaa ccggccttc
//
LOCUS       DASWNS010000192         3129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_85759, whole genome shotgun sequence.
ACCESSION   DASWNS010000192 DASWNS010000000
VERSION     DASWNS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85759"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VGB76_06515"
     CDS             <1..277
                     /locus_tag="VGB76_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098590.1"
                     /translation="YRTFLPFASGWGSPYGFGYRSSLGWPSGYYYPVRRIYRPTQPWP
                     NREPRPTMPSPRRRDYPDRTSPGMKSYPRPARPVGAGGGGGSRKRGQ"
     gene            742..1635
                     /locus_tag="VGB76_06520"
     CDS             742..1635
                     /locus_tag="VGB76_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098591.1"
                     /translation="MRRTILCGLVVLQLVVALKANGRQSWSATALPAADALATCSEKI
                     VAYADWPKVKDPLFSLELSGKTGGRLYYFGAEHLTDPAHAQFEAIERAWNALKPTVAY
                     YEGANRPIAETRDETIRQAGESGFVRFLAKRDGVPFVSLEPSPAAEAAYVMRKFPAEQ
                     VLLFYVLRETARLRERRKLPEAELKAAVAQLLERVAKMKGFEGAFADVAGLEAAYRKY
                     WTSPANWWEAPQAWFAPGKSSQATGGIFTNDINTMSSEYRDLHMYKSLAGATLEGKRV
                     FAVVGRNHVPMQSPALRCAIK"
     gene            1759..>3129
                     /locus_tag="VGB76_06525"
     CDS             1759..>3129
                     /locus_tag="VGB76_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY0098592.1"
                     /translation="MSDISLNELHATAEHDAREANFITQFPERVLREAREVAGRNRQI
                     IAQAPARDLTTLLWSSIDNSESLDLDQVEFAERLPDDRIRLLIGIADVDAFVPKESAI
                     DRHARANTVSIYTPGKVFPLLPEQLSNDTTSLREGETRLAVVTEMSISTGGAVGEVNI
                     YRALIRNRVKLVYERIGEWLDGDAPLPAEVARIEGLEAQLRLQEEAAERLRGLRLRKG
                     ALELETGEAVPVIVGGHSVELLTRERTRAQAIIENFMIAANTAMAEFLESEGLASLRR
                     VVREPERWPRIVEIAAQFEESLPARANSLALSIFLSRRRAAAPARYAELSLSILKLMG
                     AGEYLVETPDAEQDGHFGLALEDYTHSTAPNRRYADLVTQRSVKSLLAGEPAPYTVDE
                     LRAVAARCNLMESAARGVERRMRKAATALVVGERLGEEFEGVVTGVKEKGTFVKLIAP
                     PVEGKIV"
BASE COUNT          597 a          947 c         1012 g          573 t
ORIGIN      
        1 gtatcgcacg ttcctcccct tcgcttcggg ttggggttcg ccctacggct tcggttatcg
       61 ttcgagctta ggttggcctt ccggctatta ctatccggtg cggcgcatct acaggccgac
      121 ccagccttgg ccgaacaggg aaccacgccc cacgatgccg tcgccgcgac gcagggacta
      181 tcccgatagg acaagccccg gcatgaagtc ttatccgcgc ccggcgcgcc cggtcggcgc
      241 aggcggcggc gggggcagcc ggaaacgcgg ccagtaaagg gtagattctt taacaggcga
      301 caccacccgc cgggcgagca ataaccggca gggtcgggcg ggtggggtcg cagataagtt
      361 tccggctgcc agatgcagtt catcacacag ccgtgcgcgc cgcgaatttc gcaggtctta
      421 cacagtcctg cgatccgcag cgaatagccc tctccactaa atccttaccc gtgccggact
      481 caccctgcaa aagcacgttg gaggcttcag gttccgccac acgcgagcgc gcgatggcga
      541 ctgttcagcc gtcgcgttcg ccacactagt tcgcccgaac cctgaaccgt ttcgcccgtc
      601 tcacacgacg aacatgatcg ccgcaccttc tgcggacgcg tttgccgggt tgatcccgat
      661 tgcacgccgt tttcgcgtta aagggtggag caacttattc gcgtgggctg agcgggatcg
      721 ccggaagaag gagcgtgtgc tgtgcgtaga acaattttgt gcgggctagt cgtcctgcaa
      781 ctcgtcgtcg ctttgaaggc caacggtcgc cagagttggt ctgctacggc tctccccgcc
      841 gccgatgcgc tggcgacctg ttccgaaaag atagtcgctt atgccgactg gccgaaggtc
      901 aaagaccctc tcttctctct ggagctgagc ggcaagacgg gcgggcggct ctactacttc
      961 ggggcggagc acttgaccga tcccgcgcat gcacagtttg aagccatcga gcgcgcgtgg
     1021 aatgctttga agccgaccgt ggcctactac gaaggggcga accgtcccat cgcagagacg
     1081 agggacgaga cgatcaggca ggcgggcgag tcgggcttcg tgcgctttct ggcgaagcga
     1141 gacggcgtgc ccttcgtctc gctcgaacct tcgcccgccg cagaggccgc ttatgtgatg
     1201 cgaaagtttc ccgccgaaca ggtcttgctc ttctacgtgc tgcgcgaaac ggcgcggctc
     1261 agggagcggc gcaaactgcc ggaggcggag ttgaaggccg ccgtggcgca actgctcgaa
     1321 cgcgtcgcga agatgaaagg attcgaggga gcgtttgcgg acgttgccgg gttggaagcg
     1381 gcctatcgta aatactggac gagtccggcg aactggtggg aggctccgca agcatggttc
     1441 gcgccgggta aatcttcgca ggcgacgggc ggcatcttta cgaacgacat caacacgatg
     1501 tcgagcgagt atcgcgatct gcacatgtac aagtctctgg ccggggcgac gctggaaggc
     1561 aaacgtgtct tcgcggtcgt cggacgcaat cacgtcccga tgcagtcgcc cgcgctgcgc
     1621 tgcgcgatca agtagcgcgc gtcgccgttt gcagttgtgc gtgcgcatgt tgtcgctcac
     1681 atctgtaatc taccgcaggg ggacggcgcg gggtttgaaa tttgggacaa actccgcgcg
     1741 ctgctgtaga ttatagatgt gagcgacatc tccttaaacg aattacacgc gacggcggag
     1801 cacgacgctc gcgaagcaaa tttcatcacg caatttcccg aacgcgtctt gcgagaagcg
     1861 cgcgaggtgg ccggaagaaa ccggcagatc atcgcacagg cacccgcgcg cgacttaacc
     1921 acactcctct ggtcgtccat agacaacagc gaatcgctcg atctggatca agtggagttc
     1981 gcagagcgtc tgccggacga ccgtatacga ctcctgatcg ggatcgccga cgtggacgcc
     2041 ttcgtcccga aagagtcggc catcgaccgc cacgcccgcg ccaacaccgt ctctatctac
     2101 acgcccggta aagtcttccc actgctgccc gaacaactct cgaacgacac gacctcgcta
     2161 cgcgaaggcg aaacccggct cgcggtcgtc accgaaatgt ccatctcgac gggcggggca
     2221 gttggtgagg tgaatatcta ccgtgcgtta attcgcaatc gcgtgaaact ggtctacgaa
     2281 cgcatcggcg aatggctcga cggcgacgcg cctctccctg cggaggtcgc gcgcatcgag
     2341 gggctggagg cgcagttgag gttgcaggag gaggctgcgg agcgtctgcg cgggttgcgc
     2401 ctgcgcaagg gcgcgctcga actagagacg ggagaggccg tgccggtgat cgtcgggggg
     2461 cattcggtcg aactcctaac gcgcgaacgc acgcgggcgc aagccatcat cgaaaacttt
     2521 atgatcgcgg ccaacactgc gatggcagag tttttggagt cggaaggatt ggcgtcgctg
     2581 cggcgcgtgg tgcgtgagcc ggagcgttgg ccgcgcatcg tggagatcgc cgcgcagttc
     2641 gaagagagtt taccggcgcg tgcgaattcg ctcgccctct caatcttcct gtcccgccgc
     2701 cgcgcggcag ccccggcgcg ctacgcggaa ctctcgctct cgatcctgaa actcatggga
     2761 gcgggcgagt acttggttga gacgcccgat gcagagcagg acggccactt cggcctcgcc
     2821 ctcgaagact acacgcactc gaccgctccc aatcgacgct atgccgacct cgtcacccaa
     2881 cgctctgtga agtcgctgct ggcgggcgaa cccgcgcctt acacagtgga cgaactgcgc
     2941 gccgtcgccg cgcgttgcaa cttgatggag agcgcggcgc gtggcgtcga gcgcaggatg
     3001 cgcaaggctg cgaccgctct ggtggtgggc gaaaggttgg gagaagagtt tgaaggggtg
     3061 gtgaccgggg tgaaggagaa gggaaccttc gtgaaattga tcgcgcctcc ggttgagggc
     3121 aaaattgtc
//
LOCUS       DASWNS010000193         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_293155, whole genome shotgun sequence.
ACCESSION   DASWNS010000193 DASWNS010000000
VERSION     DASWNS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293155"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VGB76_06530"
     CDS             complement(<1..436)
                     /locus_tag="VGB76_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY0098593.1"
                     /translation="MERYSRQILFAGIGREGQARLRAAHVLIVGCGALGSAQAESLAR
                     AGVGRLRIVDRDFVEESNLQRQTMFTEADARERVPKAVACARRIGEINSDVEAEAHVA
                     DVHHGNIERLIENCDLVIDGTDNFATRYLINDACVKHAVNWIY"
     gene            complement(503..1012)
                     /locus_tag="VGB76_06535"
     CDS             complement(503..1012)
                     /locus_tag="VGB76_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098594.1"
                     /translation="MSRDEDEDFEELSRDRSHPRAAQLLTDEFFWDCVDENTPFGNDT
                     GADALAFFREWRRAHPHTNAPEFLAEILGEVSEVPDAHRDAVEPERVQELLAESEYDV
                     SVLDDTVIAVAFGQIVLEGRVDAEIKRRALLALQRQASPAVIRHRGWVSSAERIERLE
                     RMREVLGGL"
     gene            complement(1012..2601)
                     /gene="aroE"
                     /locus_tag="VGB76_06540"
     CDS             complement(1012..2601)
                     /gene="aroE"
                     /locus_tag="VGB76_06540"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY0098595.1"
                     /translation="MTFDTSVRLCVPVCVRRAGELRAAVARATAETQGADMIELRMDC
                     LADEAELEQARLELPDIFRAATRPLILTFRPSEQGGARASNFERRVRFWSENFSGGDE
                     RADYADLELDMAAHFKALEEQRAGTLLDWRKVICSHHDFARVPSDLEETFARMLDTPA
                     RILKLALAAEQITDSLPVFRLLKRARDAGREMIAVAMGEAGLLTRILAPARGAFLTFG
                     ALAREHATAPGQIAARDLRALYRIDLINERTEIYGLAGSPVAHSLSPQMHNAAFAALG
                     LDAVYIPFEVGDVQAFARRMAHPRTRELAWNLRGLSVTAPHKSAIIPLLDRIESSAQE
                     IGAVNTVVVEGDALSGYNTDARAALAPLSNLITVGHGSRVAVLGAGGAARAVLWSLRE
                     AGARPTVFARDTERARPTAQKFDAHLQTLDGARFDNFDLVVNTTPLGTRGARETETPA
                     LAAQLRGARCAYDLVYNPCETRFMREARAANCEFVFGGLPMLVAQAAAQFKLWTGRDA
                     PLEVLNLEAERGVTGSRPESA"
     gene            complement(2736..3257)
                     /locus_tag="VGB76_06545"
     CDS             complement(2736..3257)
                     /locus_tag="VGB76_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0098596.1"
                     /translation="MYCPKCGTQNVDDASFCRGCGVNLSLVPQALEGRAPAPVAAAAG
                     KPAHTDRHKRDDPPNLTYALVKTFVGIAFILVALSVKNVHQIAGQIWWFWMLIPAAGS
                     LGAGVAEFLRLSQQQPQAQSSRPLAGGHAYVPPAVSATPRGSELPPRRDNVDIYAPSS
                     VTEHTTKLLDKDQ"
     gene            complement(3333..>3655)
                     /locus_tag="VGB76_06550"
     CDS             complement(3333..>3655)
                     /locus_tag="VGB76_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098597.1"
                     /translation="LVIFLTGIFVFRGAFWLTIWFIIPAFGSFGEGIGQLIRARQERG
                     QLSLPPGYEGVPFQPAPLAPAAADLRRFSSPDTSEMLRSPGSVTEATTRHLDASLENG
                     AKDA"
BASE COUNT          644 a         1174 c         1162 g          675 t
ORIGIN      
        1 cgtaaatcca gttgacggcg tgcttcacac aggcgtcgtt gatgagataa cgcgtggcga
       61 agttgtccgt gccgtcgatc acgaggtcac agttttcgat gaggcgttcg atgttgccgt
      121 gatgcacgtc cgcgacgtgc gcttccgctt ccacgtccga attgatctcg ccgatgcgac
      181 gcgcgcaggc caccgctttc ggcacgcgtt cccgcgcgtc cgcttcggtg aacatcgtct
      241 gtcgttgcag gttcgactcc tcgacgaaat cacgatccac gatccgcagg cgaccgaccc
      301 cggcgcgcgc cagactttca gcctgcgccg accccaacgc cccgcaaccg acgatcagca
      361 catgcgccgc cctcaggcgc gcctgtcctt cgcgcccgat gcccgcgaat aaaatctgcc
      421 tgctgtagcg ttccatcaaa attccaccat caaccgactc gacgttaact ctcccggcct
      481 tgaattaaaa gcgcgcccga agttacagcc cgccgagaac ttcccgcatc ctctccagcc
      541 gctcgattcg ttccgccgaa ctcacccagc cgcgatgcct gatcacggct ggcgacgctt
      601 gcctctgcaa cgccagtaac gcacgccgtt tgatctccgc atccactctg ccttcaagaa
      661 cgatctgccc gaaggcgacg gcaatcacag tatcatcaag aacacttacg tcgtattcgg
      721 actcagccag taattcctgc actcgctccg gttcgacggc atcccggtga gcgtcaggaa
      781 cttcagacac ctctccgaga atctctgcga gaaactccgg cgcgttcgtg tgcgggtgtg
      841 cccttcgcca ttcgcgaaag aacgccagcg catccgcccc cgtgtcgttg ccgaaaggcg
      901 tgttctcgtc cacgcaatcc cagaagaact catccgttaa aagctgcgcc gcgcgcgggt
      961 gcgacctatc gcgcgacaac tcctcaaaat cttcatcttc gtcgcggctc atcacgcact
     1021 ctccgggcgc gaacctgtca cgccccgctc cgcttcgagg ttcaacactt cgagcggcgc
     1081 atctcgcccc gtccataatt taaactgcgc ggcggcctgc gcgacgagca tcggcagccc
     1141 gccgaagacg aactcgcaat tcgccgcgcg cgcttcgcgc atgaagcgcg tctcgcacgg
     1201 attgtaaacg agatcgtagg cgcagcgcgc gccgcgaagt tgcgcggcga gggcgggcgt
     1261 ctccgtctcg cgtgcgccgc gcgtgccgag cggcgtcgtg ttgacgacga gatcgaagtt
     1321 gtcgaagcgt gcgccgtcga gcgtttgcaa atgcgcgtcg aacttttgcg cggtcgggcg
     1381 tgcgcgctcc gtgtcgcgcg caaagacggt cggacgcgct ccggcttcgc gcaaactcca
     1441 caggacggcg cgcgccgcgc cgcccgcgcc gagcaccgcg acgcgcgacc cgtgcccgac
     1501 cgtgatcaaa ttcgagagcg gcgcgagcgc ggcgcgtgcg tccgtgttgt aaccgctgag
     1561 cgcgtcgcct tcgacaacga ccgtgttgac cgcgccgatc tcctgcgcag acgattcgat
     1621 cctgtcgagc agcgggatga tcgcgctctt gtgcggcgcg gtgacgctca ggccgcgcag
     1681 gttccaggcg agttcgcgcg tgcgcgggtg cgccatgcga cgggcgaagg cttgcacgtc
     1741 gccaacttca aaagggatgt agacggcgtc gaggccgagt gcggcgaacg ccgcgttgtg
     1801 catctgcggc gagagcgaat gtgcgacggg cgaaccggcg agtccgtaga tttccgtccg
     1861 ctcgttgatg agatcgatgc gatagagagc gcgcaggtcg cgtgcggcga tctggccggg
     1921 cgcagttgcg tgttcgcgtg cgagcgcgcc gaaggtgaga aacgcgccgc gcgcgggcgc
     1981 aaggatgcgc gtcaggagtc cggcctcgcc cattgctacg gcgatcatct cgcgtccggc
     2041 gtcgcgcgcc cgtttgagga gacgaaagac gggcagcgag tctgtgatct gctctgcggc
     2101 gagggcgagt ttcaggatgc gcgcgggcgt gtcgagcatg cgcgcgaaag tctcttcgag
     2161 gtcagaggga acgcgcgcga agtcgtgatg cgagcagatg actttccgcc aatcgagcag
     2221 cgtcccggcg cgctgttctt cgagggcttt gaagtgcgcc gccatgtcga gttcgagatc
     2281 ggcgtagtct gcgcgctcgt cccccccgga aaaattctcc gaccagaaac ggacgcgccg
     2341 ctcgaagttc gacgcgcgcg cgccgccctg ttcggacggg cggaaggtga gaatgagcgg
     2401 gcgcgtcgcc gcgcggaaga tgtccgggag ttcgagccgc gcctgttcaa gctccgcttc
     2461 gtccgcgaga caatccatgc gcaactcgat catgtctgcg ccttgcgtct ctgccgtcgc
     2521 gcgcgcgact gccgcacgca actcgcccgc gcgtcgcacg cagacgggaa cgcagagccg
     2581 gacgcttgta tcgaatgtca tcgaggaagc cggacgtgag ataaagtttt aaagctgccg
     2641 gagattcttg cgcgcactct tcttccctgc gccgggggcg tgaagttcat cttcgacagg
     2701 ccgccggatt gagcgcctaa gttgagcggg ttggtttact ggtctttgtc gagaagtttc
     2761 gtcgtatgct cggtgacgct gcttggcgcg tagatgtcta cgttgtcgcg gcgcggcggc
     2821 agttcgctcc cgcgcggcgt ggcggagacg gcgggcggca cgtatgcgtg gccgcccgcg
     2881 agcggcctcg acgattgtgc ttgtggctgc tgttgagaga gccgcagaaa ctccgcgacg
     2941 cccgcgccga gtgaaccggc ggcgggaatc aacatccaga accaccagat ttgacccgcg
     3001 atctgatgaa cgtttttcac cgacagcgcg acgaggatga aggctatgcc gacgaaggtt
     3061 ttgacgagcg cgtaagtgag attcggcgga tcgtctcgct tgtgcctgtc cgtgtgcgcg
     3121 ggtttgcctg cggcggcggc gacgggtgcg ggcgcgcgtc cctccaaagc ctgcggcacg
     3181 aggctcaggt tgacgccgca gccgcgacaa aaactcgcat cgtcaacgtt ctgtgttccg
     3241 catttcggac agtacatttt tcagtatctc cttaaaccgt cgggcatgcg cgcgacagcc
     3301 cgcgcgtcgt gcgcgctctt tcttccgttt cgtcaagcat cttttgcgcc gttttcgagg
     3361 gaggcgtcga ggtgtctggt cgtagcctct gtgacgcttc cgggcgagcg gagcatctcg
     3421 gaggtatcag gcgatgagaa tcgtctcaag tcagccgccg ccggagcgag cggcgcgggt
     3481 tggaagggca cgccctcata ccccggcggc agcgacaact gcccgcgctc ctgtcgcgcg
     3541 cgaataagtt gaccgatgcc ttcgccgaac gaaccaaagg cggggatgat gaaccagatc
     3601 gtcagccaga aggctccgcg aaagacgaaa atgccggtca gaaaaattac taaca
//
LOCUS       DASWNS010000194         7620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_162905, whole genome shotgun sequence.
ACCESSION   DASWNS010000194 DASWNS010000000
VERSION     DASWNS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162905"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VGB76_06555"
     CDS             complement(<1..1049)
                     /locus_tag="VGB76_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEY0098598.1"
                     /translation="MSTALAIASVTAVLRDLLNNGMIDQNISGTVGSEVLVTALPPDR
                     ILKEGTAETSQLNLFLYQVTPNAGWRNVGLPSRNGRGERLTNPPLALDLHYLLSAYGE
                     KDFHAEILLGYAMQLFHETPVLTRDAIRLALAPPTTVEGTSVLPTALRTLFESELAEQ
                     VEQVKLSPVSMSTEEISRLWAAFQTRYRPTAAYQASVVLIESRSGAARPALPVLSRNI
                     LAVPFHQPIIETVMSQENDAAPILADQPLLTGYNLNLVGTQLRGDDTHVQIGGVEILT
                     PLKEVTDTRITFALPPNLRAGVQGAQVVHYRSFGTPNEPHRGVASNVAAFVLRPTVGT
                     IGAATVPGQDADHVSG"
     gene            complement(1046..1927)
                     /locus_tag="VGB76_06560"
     CDS             complement(1046..1927)
                     /locus_tag="VGB76_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098599.1"
                     /translation="MSDYLKHLAARSLNRTPSMQPRVASRFEPSTLAAAPPASEPVLD
                     STQLDAEENARPLSPSGAHAPQPTENRQRTADTAPRSLDATPLSEARHESRTHNDAVQ
                     PPETSSSRLPYQTSPSNVFTPSSVREQARSPAQATPETERTPPPQASEEAHLRLRHAG
                     DPQAHRPPETNESALERSIRRIVAGEFEAREAEVAAGGSSRSHARTGKGMPPPVMPVS
                     TVASRRSPVTTREQADASAQASPPIIRVTIGRIEVRAVTPQGQRVEARSAPPAGARPD
                     PARSLREYLKQRSGGRP"
     gene            complement(1924..2658)
                     /locus_tag="VGB76_06565"
     CDS             complement(1924..2658)
                     /locus_tag="VGB76_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098600.1"
                     /translation="MRALSDSELLDVWEHALEQPPLERALTLLAAATGIDAGELAHLS
                     IGERDALLLDLREQTFGAQLSSVATCPSCNEPLEMSFRVAQLRVATPADKPPGSFSLN
                     VGGYEVSFRLPNSLDLSALASVQEVAAARRVLFERCLLAARRGDEEQTAGALPPEVVD
                     AIVERMGQVDAQGDMQFALKCPACAHQWLEAFDIDSFFWMELNVWAQRTLREVHALAR
                     AYGWRETDILSLSPQRRRFYLSMFSE"
     gene            complement(2680..3207)
                     /locus_tag="VGB76_06570"
     CDS             complement(2680..3207)
                     /locus_tag="VGB76_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY0098601.1"
                     /translation="MAQFSVNAQRFDPYKNFKFRVKWDGRYVAGISKVGMLKRSTEVV
                     KHREGGDPSTTRKSPGRTEYDAISLERGVTHDTEFERWANKVWNFGSGLGAEVSLKDF
                     RKDIIIEMYNEAGQLAIAYRVFRCWVSEYQAQPDLDANANAIAIQHIKLEHEGWERDY
                     EVNEPSEPSFTEPAS"
     gene            complement(3219..4790)
                     /locus_tag="VGB76_06575"
     CDS             complement(3219..4790)
                     /locus_tag="VGB76_06575"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEY0098602.1"
                     /translation="MPVTPTYPGVYIEEIPSGVRTISGVATSITAFIGRARRGPVNEP
                     VVINNFGDFERRFGGLSTADPMSYAVRDFYLNGGSQAIIVRLFTNAGGSGLAEINVDT
                     LQLVAASPGVWGNRLHVVVDYDGITNEVAEQYGLTAGDLFNLSVFEKATSGGSDRLLE
                     RLINLSISDNSRRVDRVLDQQSQNVRVPRAGGQPVLPASVPGDGSDGDGSNGLDSTAL
                     GQADYEGAPADKSGIYALEKADLFNLLCIPPDTRGGDVPPAVYQTAMKFCADHRAMLI
                     VDSPAAWSANKETAASNAVAGLGALGLSGIAARNAALYFPRVVETDPLREGQLDTFVP
                     CGIIAGVMARTDTQRGVWKAPAGLDATVNGVQGLEVNLSDPENGMLNPLGINCLRSFP
                     VNGRVVWGARTLRGADQLADEYKYVPVRRLALYIEESLYRGTQWVVFEPNDEPLWAQI
                     RLNVGAFMNNLFRQGAFQGRSPREAYLVKCDRETTTQNDINLGIVNILVGFAPLKPAE
                     FVIIKIQQLAGQIDV"
     gene            complement(4857..5249)
                     /locus_tag="VGB76_06580"
     CDS             complement(4857..5249)
                     /locus_tag="VGB76_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098603.1"
                     /translation="MPIKINWTFNAQVAGGPKVAAAESVEVDAYENIEVVVPKHDAND
                     GTAEVGIVPADTGEILFLLIKADNYTDTQLSYVIGDATDEVALDAQQVFIGAGAVGLL
                     GESPSKLTFTNKGPEDIAVRILVGRKAA"
     gene            complement(5726..6022)
                     /locus_tag="VGB76_06585"
     CDS             complement(5726..6022)
                     /locus_tag="VGB76_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098604.1"
                     /translation="MSDKTTNQTGENEDLQPTDLVIRVNTVLTNDPCALCGQPTDPTG
                     IDLFLAERPALVCHSCGFFHAPALARLLDLASAANTFVRAEYEMFGIQSDDKPV"
     gene            complement(6362..7186)
                     /locus_tag="VGB76_06590"
     CDS             complement(6362..7186)
                     /locus_tag="VGB76_06590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEY0098605.1"
                     /translation="MTNNSNSDVRSLTALQLKSDFVARGSIVRKIWGDTELVLLIFAG
                     SAAEFALNRAVDWLFFTGKIPADPIGRLFSTVRYAQEIVFAEREAAERTLDRINAVHG
                     AVERQRGQSIPDWAFRDVLYMLVDYSERAHSMLYRPLSRAEREELYLVFKRVGEGLNV
                     KALPESYEEWRADRRLHLVRDLNHSRHTALLYGQYRRHLGEWRYQILRGVQALLVPER
                     VRRLLRLRRRTIFSALVEGYGQLVVPELRPLVRRLMVQPRYWDEMKKMERGLSKDA"
     gene            complement(7352..>7620)
                     /locus_tag="VGB76_06595"
     CDS             complement(7352..>7620)
                     /locus_tag="VGB76_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098606.1"
                     /translation="AFAGRYQSDELGAFFDMAPGKDGLKGRANDSPVALPEFRPVDRD
                     TFQLGGVILRFLRDKAGKVVALDYSNPLVRNIKFTRLSDRPGRP"
BASE COUNT         1391 a         2324 c         2399 g         1506 t
ORIGIN      
        1 ccagagacgt gatcggcatc ctgccccggc acggtcgccg cgccaatcgt cccgaccgtg
       61 ggacgcagga cgaacgccgc gacgttcgag gcgacgccgc gatgcggctc gttcggcgtc
      121 ccgaacgaac ggtagtgcac cacctgcgcg ccctgtacgc ccgcgcgaag gttcggcggg
      181 agggcgaagg tgatgcgcgt gtctgtgacc tctttgaggg gcgtcaaaat ctcgacgccg
      241 ccgatctgga cgtgcgtgtc gtcgccgcgc aactgcgtgc cgacgagatt caggttgtag
      301 ccggtaagga gcggctggtc tgcgaggatc ggcgcagcgt cgttctcctg cgacatgaca
      361 gtctcgatga tcggttgatg gaagggaact gcgaggatgt ttcgactgag cacgggcagc
      421 gcagggcgag ccgcgccgct ccggctctcg atcaggacga cggatgcctg gtaggccgcc
      481 gtcgggcgat agcgcgtctg aaaggccgcc cagaggcgcg aaatctcttc cgtgctcatc
      541 gagacggggg agagcttcac ctgctcgacc tgctcggcca attccgactc gaacagagtg
      601 cgcaaggccg tgggcaggac ggacgtgcct tcgacggtcg ttggcggggc gagcgcaagg
      661 cgtatcgcgt cgcgcgtcag cacgggcgtc tcgtggaaca actgcatggc atagccgagc
      721 agaatctctg cgtggaaatc cttttcgcca taagccgaca gcaggtagtg caggtcgagc
      781 gcgagcggcg ggttcgtcaa acgctcgccc cgcccgttac gcgaagggag gccgacattg
      841 cgccagcccg cgttcggcgt gacctgatag agaaacaggt tgagctggct tgtttccgcc
      901 gtcccctcct tgaggatgcg gtcgggcggc aaggccgtga cgagcacttc gctccccacc
      961 gtgccggaga tgttctggtc aatcatgccg ttgttgagca gatctctcag cacggctgtg
     1021 accgacgcaa tggcaagggc ggtgctcacg gtctacctcc gctgcgctgt ttgagatatt
     1081 cccgtagcga gcgtgccggg tcgggtcgcg ctccggcagg cggcgcactt cgcgcctcga
     1141 cccgctgccc ctgcggggta acggcacgca cttcgattcg cccgatggtg acgcggatga
     1201 tcgggggcga agcctgcgcg gaagcgtcgg cctgctcgcg cgtcgtcaca ggcgaacgcc
     1261 gcgacgcgac tgtcgagacg ggcatgaccg gcggaggcat gccctttccg gtacgagcat
     1321 gtgagcgcga cgagccgcct gctgcgactt ccgcctcgcg cgcctcgaac tctccggcga
     1381 cgatccggcg gatacttcgt tcgagcgcgg attcattcgt ctcaggcggg cggtgcgcct
     1441 gcgggtcgcc cgcgtgccgg agtctcaggt gcgcctcttc cgacgcctgt ggcggaggcg
     1501 tgcgctccgt ctcaggcgtg gcttgagcag gcgagcgtgc ctgctctcgc acggacgacg
     1561 gggtgaaaac gttggatggc gatgtttgat aaggcagcct tgacgaagag gtttccgggg
     1621 gctggacggc atcattgtgc gtgcggcttt cgtggcgcgc ttcggagagc ggagttgcgt
     1681 cgagcgagcg tggtgcagtg tccgccgtgc gctgccggtt ctccgtgggc tgaggcgcgt
     1741 gtgcccctga cggagagaga gggcgtgcgt tctcttccgc gtcgagttgg gtgctgtcga
     1801 gcacgggttc ggatgcgggc ggcgcggcgg ccagcgttga aggctcgaag cgcgacgcga
     1861 cgcgtggctg catggagggc gtgcggttaa gactgcgtgc ggccagatgt ttgagataat
     1921 cgctcattcg ctaaacatgc tcaggtagaa acggcgtcgc tgcggactca gggaaagaat
     1981 gtccgtctcg cgccagccgt aggctcgtgc gagcgcatgc acctctctca gcgtgcgctg
     2041 cgcccagacg ttaagctcca tccagaagaa agagtcgatg tcgaaggctt ccagccattg
     2101 gtgcgcgcag gccgggcact tgagtgcaaa ctgcatatct ccctgcgcgt caacctgccc
     2161 cattcgctcg acgattgcgt ccacgacttc cggcggtagc gcgcctgccg tctgctcctc
     2221 atctccgcgc cgcgccgcga gcaggcatcg ctcgaagagc actcgccgcg ccgccgcgac
     2281 ctcctgcacg gaggcgagcg cggagaggtc caggctgttc ggcagacgaa agctcacttc
     2341 gtatccgccg acgttgagcg agaaagagcc gggaggtttg tctgcgggag tcgccacacg
     2401 caactgtgcc acacggaaac tcatctccag cggctcgttg caggacgggc acgtcgccac
     2461 gctcgatagc tgcgcgccga aggtctgttc ccgcaggtcg agcagcagcg cgtcgcgctc
     2521 gccgatgctt aaatgcgcca actctccggc gtctattcct gtcgcagcgg cgaggagcgt
     2581 gagcgcgcgt tcgagcggag gctgttcgag cgcgtgctcc cacacgtcga gcaactccga
     2641 atcggacagg gcgcgcatca ctcaatcact tcgtttcgac taagacgcgg gttcggtaaa
     2701 cgacggctcg gacggctcgt tcacttcgta gtctcgctcc cagccctcgt gctccagctt
     2761 gatgtgctgg atggcgatgg cattggcgtt ggcgtccaag tcgggctgcg cctggtattc
     2821 cgaaacccag cagcgaaaga ccctgtaagc gatggcgagt tgtcccgcct cgttgtacat
     2881 ctcgatgatg atgtccttgc ggaaatcctt gagcgagact tccgcgccaa gaccggagcc
     2941 gaagttccag accttattcg cccaacgctc gaattccgta tcgtgggtga cgccccgttc
     3001 gagagagatg gcgtcgtact cggtgcgacc cggcgacttg cgcgtcgtgc tcgggtcgcc
     3061 gccctcccga tgtttgacga cttcggttga acgcttgagc atgccgactt tgctgatccc
     3121 ggcgacgtaa cgcccgtccc acttgacgcg aaatttgaag ttcttatacg gatcgaaacg
     3181 ctgtgcgttg acgctgaact gagccatgac ttttctcctt agacatcaat ctgtccagcg
     3241 agctgctgaa tcttgatgat cacaaactcg gcgggcttga gcggcgcgaa gccgacgagg
     3301 atattgacga tgccgagatt gatgtcgttc tgcgtcgtcg tctcccggtc gcacttgacg
     3361 aggtaggcct cgcgcgggct gcgaccctga aaggctccct gccggaacag gttgttcata
     3421 aacgcgccga cgttgaggcg aatctgcgcc cacagcggtt cgtcgttcgg ctcgaagacc
     3481 acccactgcg tccccctgta gaggctctcc tcgatgtaga gcgcgagcct acggacgggg
     3541 acatacttgt actcgtcggc caactggtct gcgccgcgca gcgtgcgcgc gccccatacg
     3601 acgcgaccgt tgacgggaaa cgaacgcagg cagttgatgc cgagtggatt aagcatgccg
     3661 ttttccgggt cgctcaggtt cacttccaaa ccctgcacgc cgttgacggt ggcgtcgagt
     3721 ccggcgggcg ctttccagac gccgcgttgc gtgtctgtgc gcgccatcac cccggcgatg
     3781 atgccgcagg gcacgaaggt atcgagttgg ccttcgcgca gtgggtctgt ctcgacgacg
     3841 cgcgggaagt agagcgcggc attgcgcgcc gcgatgccgg agaggccgag cgcgccgagt
     3901 ccggccacgg cattgctggc cgccgtctct ttattcgcgc tccatgcggc gggcgagtcc
     3961 acgatgagca tggcgcgatg gtcggcgcag aacttcatgg ccgtctggta gacggcaggc
     4021 ggcacgtcgc cgccgcgcgt gtcgggcggg atgcagagca gattgaagag gtcggccttc
     4081 tccaaagcat aaatgcctga cttgtccgcc ggcgcgcctt cgtaatccgc ctgtccgagc
     4141 gcggtgctgt cgagtccgtt cgagccgtcg ccgtccgagc cgtcgccggg aacagaggcg
     4201 ggcagaaccg gctggccgcc cgcgcgcggg acacgcacgt tctgcgactg ttgatccagc
     4261 acgcggtcca cgcgccgcga gttgtcgctg atggagaggt tgatcaaacg ttcgagcaga
     4321 cggtcggagc cgccgctcgt cgctttttca aagaccgaga ggttgaacaa atctcccgcc
     4381 gtgaggccgt actgctcggc cacctcgttc gtaatgccgt cgtaatccac gacgacgtgc
     4441 aggcggttgc cccacacgcc gggactggcg gccaccagtt gcagcgtatc gacgttgatc
     4501 tcggcaaggc cgctgcctcc ggcgtttgtg aagaggcgaa cgatgatggc ctggctgccg
     4561 ccgttgagat aaaaatcgcg taccgcgtaa ctcatagggt cggcggtgga gaggccgccg
     4621 aagcggcgtt cgaagtcgcc gaaattgttg atgacgaccg gctcgttaac aggaccacgc
     4681 cgtgcgcgac cgatgaaagc cgtgatggag gtggcgacgc cggagatggt gcggacgccg
     4741 ctcggaatct cttcgatata gacaccggga taggtcggtg tgactggcat gaaacattct
     4801 ccttctcaag tatggcgggt gcggaacgaa cgactttcca aagcagacag cgcgcgtcag
     4861 gccgccttcc gcccgacgag gatgcgcacg gcgatatctt ctggtccttt gttcgtaaag
     4921 gtgagcttgg acggcgactc gccgagtaaa ccgaccgcac ccgcgccgat aaagacctgc
     4981 tgcgcgtcga gcgcgacctc atcggttgca tcgccgatga cgtagctcag ttgtgtgtcg
     5041 gtgtagttgt cggctttgat caagaggaac aggatttcgc ccgtgtctgc cgggacgatg
     5101 ccgacttcgg cggttccgtc attggcgtca tgcttgggca cgactacttc gatattctcg
     5161 taggcatcaa cctcgacgct ttcggcggcg gccactttcg gcccgcctgc gacctgagcg
     5221 ttgaacgtcc aatttatttt gatcggcatg atttactccc tcaacgttgc tgtcaacata
     5281 gcccttgcgc ggcctcttcg gccacggcgg gcggcgaaaa ttcagttccc ccggttcgtc
     5341 aacctcatgg aacacttccc gcccacctga gacgaagcag acctacggcg attcgtcacc
     5401 aagcgtcagg aaaatttgcc ccgtgaccgc acgtcccggc gcatctgttc tttatttttt
     5461 gaaaaaccaa gatgtagcgt ctgaaggaat gtactgaatc cttcctgcat tagtgagtac
     5521 aacaggcaga aaaaccgacg aggataaatt tctattcttg cgtgaaaaca ccagagcgcg
     5581 aggaagacct tagaagttct atgaagcaag cggagagtat tctgacacgc ctcaggctgt
     5641 caacgttttt ctttcgccac gccaaaaaca ccgccagcgg agggcaggcc gtcggtacaa
     5701 gtccgctcct gattcctccg aacttttata ccggcttatc atccgattgg atgccgaaca
     5761 tctcatactc ggcgcggaca aaagtgttag ccgcgctcgc cagatcaagc aagcgtgcga
     5821 gagccggggc gtggaaaaat ccgcagctat gacaaacgag agcgggtcgt tctgcgagga
     5881 agagatcgat gccggtcggg tctgtcggtt gtccgcacag ggcgcaagga tcatttgtca
     5941 ggacagtgtt gaccctgatc actaaatcag tcggttgaag gtcttcattc tcacccgttt
     6001 ggttcgttgt tttatcgctc acttaatgat tatccctttc gaaacggtaa tgtcgcaaaa
     6061 cagccattcg ggtcttatct ctccctcacg ctgcgtcaac tgatattatc agttctccgc
     6121 atgaagcata aatcctcgcc tttcaaatga cggcagcacg cgccgcagac cgacacccgc
     6181 ccgtgcgcca cgccgctcgc ggctagttcg tcgcgtgcgg tttcgactgc catgaaagcg
     6241 ggcgcgaagt tcactgaacc tcgcgcccgc tcttcatgga gtcttctaaa cgcaattatg
     6301 cgatagcagt gttgcttcct caggtatgca cggaaggtcg gagagattgc gcccgaacgc
     6361 ctcaggcatc tttcgataga ccgcgctcca tcttcttcat ctcatcccag tagcgaggct
     6421 ggaccatgag acgccgtacc agcgggcgaa gctcggggac gactaactgc ccgtaccctt
     6481 cgaccagcgc ggagaagatc gtgcgccgcc ggagccgcag caggcggcgg acacgttcgg
     6541 gcaccagtaa cgcctgcacc ccgcgaagta tctggtaacg ccactcgccc aggtgtcgtc
     6601 ggtactggcc gtagaggagc gcggtgtgtc tgctgtggtt gaggtcgcgc acgaggtgga
     6661 gtcgcctgtc ggcacgccac tcctcgtaac tttccggcag tgccttgacg ttcaacccct
     6721 ctccgacgcg cttgaagact agatagagtt cttcgcgctc agcgcgcgag agcggtctgt
     6781 agagcatgct gtgtgcccgc tcggagtagt cgacgagcat gtagagcacg tccctgaaag
     6841 cccagtcggg gatgctctgc ccgcgctgac gctcgaccgc gccgtggacc gcgttgatcc
     6901 tgtcgagcgt ccgttccgcc gcctccctct ccgcgaacac gatctcctgc gcgtagcgaa
     6961 ccgtggagaa cagacgcccg atggggtccg ccggtatctt gcccgtgaag aagagccagt
     7021 caaccgccct gttcaaggcg aactcggcgg ctgagccggc gaagatcaac agtaccaact
     7081 ctgtgtcgcc ccagatcttt ctgacgattg agccgcgcgc gacgaaatcc gacttgagct
     7141 gtaacgccgt cagacttctc acgtcagagt tgctgttatt agtcacacgc agcctctctt
     7201 tctaaagcga cgaactcctc accacgtcca gaatctcttc ggggtcgctg cctgagaaga
     7261 tgcaccatag agcctcctct cgcctcagag caattcccgc aacacaaatc ttctcataga
     7321 aactgtcaac gaacttcctt ccctccggtc gctaagggcg accggggcgg tcgctcaggc
     7381 gcgtgaattt gatgttgcgg acgagggggt tgctgtagtc gagcgccaca acctttccgg
     7441 ccttgtcccg caggaagcgc aagatcactc cgccgagttg aaaggtgtcg cggtcgaccg
     7501 gcctgaattc gggcagtgcg accggtgaat cgttggcacg ccccttcaag ccgtcttttc
     7561 cgggtgccat gtcgaaaaat gccccgagtt cgtcgctttg atagcgtccg gcaaaagcct
//
LOCUS       DASWNS010000195         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_345328, whole genome shotgun sequence.
ACCESSION   DASWNS010000195 DASWNS010000000
VERSION     DASWNS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345328"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1827
                     /locus_tag="VGB76_06600"
     CDS             <1..1827
                     /locus_tag="VGB76_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0098607.1"
                     /translation="LTLTIGARISIKIPRVSGHISVSDTFIFLTIFLFGGEVAVLLAA
                     LDASVTSLRISKKLSTLIFNSATMAVSTFATYACVKYVCGDIVSLTHAPYSPLFMIAA
                     SIMASVQYVVNSGTVAIAGALKFNEPFWDSWRRKYLWTSLTYIAGASAAAILAKLIGV
                     TGFYAFIGTLPFIGVVYFTYLTYMKNVEAAATQAEQAERHVEELSHYIAEQERLRQQF
                     SQLEKLSALGELASGVAHDFNNTLAGILGRAQLMLRTDDREKILRGLQIIIKTAEDGA
                     KTVRRIQDFARQRRDHDFAPVSVDQLLFDVGELTRPRWKDRAQANNVYIRLNLQINSN
                     ALIRGDESELREVLVNMVFNAVDAMPAGGTLTLATNEDEEHVVISVSDTGCGMTPDVR
                     SRIFDPFFTTKGKAGNGLGLAVSYGIILRHEGTIEVVSEAGSGSTFNIRMPVAKGALR
                     APHAETEPPPVTTSKSLSLVGEAPLRPRLLVVDDEPHVRTLLCEMIQSENCEAVAASN
                     GTEALALFDAENFDAVFTDIGMPGMSGWELSRLIRTRNARIPLAVITGWGDVVGSDEQ
                     GAERIDWVVTKPFSLKQITEIIEEIARRKAETCESRLADVAA"
     gene            complement(1897..2292)
                     /locus_tag="VGB76_06605"
     CDS             complement(1897..2292)
                     /locus_tag="VGB76_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098608.1"
                     /translation="MSAILVVDDDDAIRDTLYDLLSENHLCHVAATAERALEWLATDV
                     YDVVLTDISMPGLSGIELLGHVRQIQPQTPVIIISGIQNEDHARGMINLGAFDYLLKP
                     FRLEDVERSVAQAIQYHARLLRGEAGNAT"
     gene            complement(2431..>2800)
                     /locus_tag="VGB76_06610"
     CDS             complement(2431..>2800)
                     /locus_tag="VGB76_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEY0098609.1"
                     /translation="GRVTGRSEVTFNFDRIRFRDGRTSSFAGFVESVRTVNGETVRVD
                     NEGTVQDSSQTDRTVQRTAIGTAVGAIIGAIAGGGKGAAIGAVVGAGGGAGSVYVQGS
                     DDLDLRSGTEITIRASGPNQ"
BASE COUNT          564 a          878 c          791 g          567 t
ORIGIN      
        1 ctaacgctca ccatcggcgc acgcatcagc atcaagatcc cgcgcgtgtc cggtcatatc
       61 tcagtctccg atacattcat tttcttaacc atcttcctct tcggcggcga agtggcagtg
      121 ctgctcgctg cgctcgatgc atccgtcacg tcgcttcgca tcagcaaaaa gctctccacc
      181 ctgatcttca actccgcgac gatggccgtc tcaaccttcg ccacctacgc gtgcgttaaa
      241 tatgtctgcg gcgacatcgt ctcattgacc cacgctccct attcgccgct ctttatgatc
      301 gctgcgagca tcatggcctc cgtgcagtac gtggtaaatt ccggcacggt cgccatcgcc
      361 ggggctttga agtttaacga gcctttctgg gattcgtggc gcaggaaata cctatggact
      421 tcgctcacct acatcgcggg cgcgtccgcc gccgccatcc ttgccaaact catcggcgtc
      481 accggtttct acgccttcat cggcacgctc cccttcatcg gcgtcgtcta tttcacttac
      541 ctcacttaca tgaagaacgt agaggcggcg gccactcaag ccgaacaggc cgaacgccac
      601 gtcgaagagt tgtcgcatta cattgccgaa caggaacgtc tgcgccagca gttttctcaa
      661 ctcgaaaagc tgtccgcgct cggcgaactc gcttcgggcg tcgcgcacga tttcaacaac
      721 acgctcgccg gaatactcgg tcgcgcccaa ttgatgttgc gcaccgacga ccgggaaaaa
      781 atcctgcgcg gcctccagat catcatcaag acggcggaag acggcgcgaa gaccgtgaga
      841 cgcatacaag acttcgcacg ccagcgtcgc gaccatgatt tcgcgcccgt ctcggttgac
      901 caactgctgt tcgatgtcgg cgagttgacg cgcccgcgct ggaaagaccg cgcgcaggcc
      961 aacaacgtct atatccgcct caatttgcag atcaactcca acgcgctcat tcgcggcgac
     1021 gagtcggagt tgcgcgaagt gctggtcaac atggtcttca acgcggtgga tgcgatgccc
     1081 gcgggcggga cgctgacgct cgcgacgaat gaggacgaag aacatgtcgt catcagcgtg
     1141 tccgatacgg gctgcggcat gacgccggat gtgcgttcgc gcatcttcga tccgttcttc
     1201 acgacgaaag gcaaagccgg aaacggcctc ggtttggccg tcagctacgg catcatcctc
     1261 cggcacgaag gcaccatcga agttgtctcc gaagcgggaa gtggttcgac ttttaatatc
     1321 aggatgccgg tggctaaagg cgcgctgaga gcgccgcacg cggagacgga accgccgccc
     1381 gtcacgacat ccaagagcct ctcactggtg ggcgaggctc cgctgcgacc gcgccttctc
     1441 gtcgttgacg acgaaccgca cgtccgcacc ttgctctgcg agatgattca aagcgagaac
     1501 tgcgaggcgg tggcggcgag caacggaacc gaggcgctcg cgctcttcga tgcggaaaac
     1561 ttcgacgcgg tcttcacgga catagggatg ccgggcatga gcggatggga actctcgcgc
     1621 ctgattcgca cgcgaaacgc acgcatcccg ctcgcagtga ttacgggatg gggcgatgtg
     1681 gtggggtctg acgaacaggg agcggagcgc attgattggg tcgtgacaaa acccttttcg
     1741 ttgaaacaga tcaccgagat catagaagag atcgcgcggc gcaaggccga aacgtgcgag
     1801 agcaggttgg ctgacgttgc cgcctaaagc gcgtcgcgtc gaacgtcgcc gggcgtgcgc
     1861 tccgcgcatt ccagtcgagt ccactcacac aaagtttcaa gtcgcgttgc ccgcctcgcc
     1921 cctgagcagt ctggcgtgat attggatggc ctgcgctacg ctccgttcga catcttccaa
     1981 cctgaaaggc ttcaacaagt agtcgaaggc tccgagattg atcatgcctc tggcgtggtc
     2041 ttcattctgt atgccggaga tgatgatgac cggggtttgc ggctggattt gccgcacgtg
     2101 gccgagcagt tctatgccgc tcaatccggg catggaaata tccgtcaaca ccacatcgta
     2161 gacatctgtg gctagccact ccaacgcacg ttcagccgtc gcggccacgt ggcagaggtg
     2221 gttttccgat aagaggtcgt agagcgtatc gcggattgcg tcgtcgtcat cgacaacaag
     2281 aattgctgac atagagtgag gataacataa aatttttcca gttttcactt tacgcaaaaa
     2341 gcgggagaag attttcgccc gcccgtcttt ttacgacggc aaacgaacat cttctcccgc
     2401 tcaagttgcg ctcgaagtgg tcagcgttga ctactgatta ggaccgctcg cgcggatcgt
     2461 gatctccgtg ccgctgcgca gatcgaggtc gtcgctgccc tgcacgtaga ctgaacctgc
     2521 gccgccaccc gcgccgacca ctgcgccgat ggctgcgccc ttaccgccgc cagcgattgc
     2581 gccgatgatc gcgccgaccg ccgtgccgat ggccgtgcgt tgcaccgtgc gatccgtctg
     2641 gctgctatcc tgcaccgtgc cttcgttatc cacacgcacc gtctcgccgt tcaccgtgcg
     2701 cacgctctcc acgaagcccg cgaagctcga cgtgcgaccg tcgcggaagc ggatgcggtc
     2761 gaagttgaaa gtcacttccg agcgacccgt aacgcgaccc
//
LOCUS       DASWNS010000196         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_8886, whole genome shotgun sequence.
ACCESSION   DASWNS010000196 DASWNS010000000
VERSION     DASWNS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8886"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VGB76_06615"
     CDS             <1..706
                     /locus_tag="VGB76_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0098610.1"
                     /translation="NDRGERPTLRLKMEAKFLPDAESANVVAEIRGSEKPDEVVLVSG
                     HFDSWDVGQGAHDDAGGCIIAWETVRLLKELGLRPRRTIRAVLYTNEENGLRGGTAYL
                     EAHRAELAKHILAIESDSGTYRPEGFGLAAAASPQARADLEEIAKLLGGIRASRIAPN
                     GGGADIGPLMRAGVIGASLDVDGTHYFDIHHTPADTLDKIDPQDLALCVAAMAVMAYT
                     VADMPTALNASTPASK"
     gene            734..1840
                     /locus_tag="VGB76_06620"
     CDS             734..1840
                     /locus_tag="VGB76_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098611.1"
                     /translation="MLKATVLALASFLLLQTGIERSGSDWNLQQSNTKVRLRGISAVS
                     HFVAWASGERGTYARTTDAGQTWRVGTVPEASELDFRDVDAFDEKTAYLLSIGEGEKS
                     RIYKTTDGGAHWTLQFKNTRPTAFFDAMAFWDRDHGIAMSDPVDGRFLIITTSDGGAT
                     WRETPAAGMPPALAGEGGFAASGTCLTVEGKRNVWFGTGGTSGARVFRSTDRGRTWKV
                     ANTSIASGKSAGIFSIAFRDARNGVVVGGDYTKESESKNNAATTRDGGKTWTTINAAP
                     PNGYRSCVVYVRNGERVPMLLAVGPSGTDYSTDQGASWKRFSGADGFHTASFAPASGA
                     VWAAGEQGRIGITYISPSPAPEKRKGARRQPNGK"
     gene            complement(1893..2957)
                     /locus_tag="VGB76_06625"
     CDS             complement(1893..2957)
                     /locus_tag="VGB76_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY0098612.1"
                     /translation="MSRLFSTFKLRDVEFRNRVFVSPMCQYSSRDGLPNEWHLVHLGS
                     RAVGGAGLVIAEATAVSPEGRISPGDTGLWSDEHTRAFRPITSFIKAQGAAAGIQLAH
                     AGRKASTDVTWRGGQPLTESEGGWQTLAPSALPFAASHPIPREMNAREIAEVVTQFVD
                     AARRSVEAGFDVIELHMAHGYLLHEFLSPLSNRRQDEYGGSLEARARLPLQVAEAVRK
                     ACPASLPIFVRISATDWADGGWELEQSIQFSRWLKEAGVDLVDCSSGALISGVRIPVK
                     AGYQVPFAAAIRREAEVATGAVGMITEATQAEQIIASGEADAVFLARALLRDPYWPLH
                     AAKELGADAPWPVQYARAVN"
     gene            2932..>3188
                     /locus_tag="VGB76_06630"
     CDS             2932..>3188
                     /locus_tag="VGB76_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098613.1"
                     /translation="MNVENNLLIHFPPAHKPVGATLLLTVDRRHARFRIEPPSSISRY
                     DQYDGRRRRRCFGIAAAGASQRFHVDDEAIAHIAFDRPLVG"
BASE COUNT          614 a         1023 c          995 g          556 t
ORIGIN      
        1 gaatgatcgc ggcgagcgtc ccacgttgcg gctcaagatg gaggcgaagt ttctgccgga
       61 tgcggagtcg gccaacgtcg ttgcggagat cagggggtcg gagaagcctg acgaagtggt
      121 gctcgtcagc ggtcatttcg attcgtggga cgtgggacag ggagcgcacg atgacgccgg
      181 aggctgcatc atcgcctggg agacggtgcg cctgctcaag gaactcggcc tgaggccgcg
      241 acgcacgatc cgcgccgtgc tctacacgaa cgaagagaac gggctgcgcg gcggcacggc
      301 ctacctcgaa gcccaccgcg ccgaactcgc gaaacacatt ctcgccatcg agtcggattc
      361 gggaacgtat cgtcccgaag ggttcggcct cgcggcggcg gcctcgccgc aagcgcgcgc
      421 cgacctcgaa gagatcgcca aacttctcgg cggcatacgc gccagccgca tcgctccgaa
      481 cggcggcggc gcggacatcg gaccactcat gcgcgcgggc gtcatcggcg cgagtctcga
      541 cgttgacggg acgcactact tcgacataca ccacacgcct gccgacacgc tcgacaaaat
      601 tgatccgcaa gacctcgctc tctgcgtcgc cgcgatggcc gttatggctt acacggtcgc
      661 agacatgccg acggcgttga acgcttcgac gcccgcgtcg aaatgagttg aacgaatcga
      721 cctttgcaaa catatgttga aagcaacagt tctcgcactc gcatcctttc ttctcctgca
      781 aaccggcatc gagcgttcgg ggtcggattg gaacttgcaa cagagcaaca cgaaagtccg
      841 cctgcgcggc ataagcgcgg ttagtcactt cgtcgcgtgg gcgagcggcg agcgcggcac
      901 gtatgcgcgc acgacggacg ccgggcagac atggcgggtc gggactgtgc ccgaagcgtc
      961 ggaactcgac tttcgcgacg tggatgcgtt cgacgaaaag actgcttacc tgctctcgat
     1021 tggcgaaggc gaaaagtcgc ggatttataa gacgaccgac ggcggcgcgc actggacgct
     1081 gcaattcaag aacacacggc ctacggcctt cttcgacgcg atggccttct gggatcgaga
     1141 tcacggcatc gccatgagcg accccgtgga cggacgcttc ctcatcatca ccacttcgga
     1201 cggcggcgcg acgtggcgcg agacgcccgc cgcagggatg cctcccgcgc tcgccggaga
     1261 aggaggcttt gccgcgagcg gcacttgcct cacggtcgaa ggcaagcgga acgtctggtt
     1321 cggcacgggc ggcacgtcgg gcgcacgcgt ctttcgttcg acggatcgag gccgcacctg
     1381 gaaagttgcg aacacgtcta tcgccagcgg caagagcgcg ggcatcttct cgattgcttt
     1441 tcgcgacgca cggaacggcg tcgtcgtggg cggggattac acgaaagaga gtgagtcgaa
     1501 gaacaacgcg gcgacgacgc gcgacggcgg caagacctgg acgacgatca acgccgcgcc
     1561 gccgaacggt tatagatcat gcgtcgtcta cgtgcgcaac ggcgaacgcg tcccgatgct
     1621 cctggcggtc gggccgtccg gcacagacta ctcgacggat caaggagcga gttggaagcg
     1681 tttcagcggc gcggacggtt ttcacaccgc gagttttgcc cccgcgtccg gcgcggtttg
     1741 ggctgccggt gagcagggtc gtattggcat cacctacatc tcgccgtcgc ccgctcctga
     1801 gaagagaaag ggcgcgcgcc gccagccgaa cggaaaatag ttacgagacg gcgtagcttt
     1861 gaacgtctcg cgctctttga ttccgctgtt cttcaattta ctgcgcgagc gtattgcaca
     1921 ggccacggcg catcggctcc gagttctttc gccgcgtgga gaggccagta ggggtcgcgc
     1981 agcagggcgc gtgcgagaaa gaccgcgtcg gcctccccgc tcgcgatgat ctgttcggcc
     2041 tgtgtcgctt cggtaatcat cccgaccgcc cccgtcgcaa cctccgcctc gcggcgaatc
     2101 gccgccgcga acggaacttg ataacccgcc ttgaccggaa tccgcacgcc ggagatgagc
     2161 gcgccgctcg aacaatcaac cagatcaact cccgcttcct tgagccagcg cgaaaactgt
     2221 atggactgct ccaactccca gccgccgtct gcccagtccg tcgcagagat gcggacgaag
     2281 atcggcaacg aagcgggaca cgccttgcgc acggcttccg ccacctgcaa aggaagacgc
     2341 gcgcgggctt cgagactgcc gccgtactcg tcctgacgcc ggttggagag cggcgacaaa
     2401 aactcgtgca gcaggtagcc gtgcgccatg tgcagttcga tcacgtcgaa gcccgcctcg
     2461 acactgcgcc gtgccgcgtc aacaaattgc gtgacgactt ctgcgatctc ccgcgcgttc
     2521 atctcgcgcg ggattggatg gctcgcggcg aaaggcaatg cgctcggagc taaagtttgc
     2581 cagccgcctt ccgactctgt gagcggttgg ccgccgcgcc acgtcacatc ggttgaggct
     2641 ttgcgccccg cgtgtgcgag ttgaatcccc gccgccgcgc cctgcgcttt gatgaaagag
     2701 gtgatgggac ggaaagctct cgtatgttca tcagaccaca gccccgtgtc gcccggtgag
     2761 attcttcctt cgggcgacac ggctgtcgct tccgcgatca cgagtcccgc gccgcccacc
     2821 gcacggctgc cgagatgtac gaggtgccat tcgttaggca agccgtcgcg gcttgaatac
     2881 tggcacatcg gcgagacgaa gacgcgattc ctgaactcga catctcgcag cttgaacgtg
     2941 gaaaacaacc tgctcataca cttccccccg gctcacaaac cagtcggcgc gacactgctg
     3001 ttaaccgttg accgccgcca tgcccgcttc cgcatcgaac ctccttcgtc aatcagtcgc
     3061 tatgatcaat atgacgggcg acggcggcga cgatgttttg gcatcgccgc cgccggagcc
     3121 tcacaacgat ttcatgtcga tgacgaagcg atagcgcaca tcgcttttga tcgtccgctc
     3181 gtaggctt
//
LOCUS       DASWNS010000197         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_448634, whole genome shotgun sequence.
ACCESSION   DASWNS010000197 DASWNS010000000
VERSION     DASWNS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448634"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VGB76_06635"
     CDS             <1..601
                     /locus_tag="VGB76_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098614.1"
                     /translation="LIGILFPLVAHRIAWVRWVSLAALCSLALLLPLNIFLFHYLFLF
                     ALGILAFFCSSGKIGRRTFVLALPAFCAGLYLTMGAAIAAVGAAVAASIVFVPLNNSF
                     LNFFGAISYSLYLLHVPLGGKVINLGARFEGSSLSKILTLLAAFGLSVGAAYLFYRFV
                     ERPSQRWCSSISYRRAAKPEESYSPTAATSLCTPVGVAE"
     gene            610..1746
                     /locus_tag="VGB76_06640"
     CDS             610..1746
                     /locus_tag="VGB76_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0098615.1"
                     /translation="MDERLAPLCYVTKPGPSELGGEYAEVGLRGKRLRLAITADPEIA
                     VPPTLYGGIERIIDMLVRGLVSRGHDVTLFAHPDSVVPCPVVAYPWTTFKSRTGTLRN
                     TLLVSTNILRGRFDIVHSFGRLAYMMPILPISGPKIMSYQREVTERSVIWGTRLSRGT
                     LHFTGCSRHLVQQFLGNSNCHVVYNGVPADTYKLRESVSVDAPLVFLGRVEEIKGPHL
                     AVEIARRSGRSLVIAGNIPEGEAHQAFFKTHVAPYVNGEQVRYVGPVNDAEKNELLGK
                     AAAFLMPILWEEPFGIVMAEALACGTPVIGLNRGSVPEIVKHGVNGFVCGSIDEMIAA
                     VGRLNEIDRAACRRIMEEKFSDMAVVDAYENLYFKLLGTAHPYA"
     gene            1754..2500
                     /locus_tag="VGB76_06645"
     CDS             1754..2500
                     /locus_tag="VGB76_06645"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0098616.1"
                     /translation="MAKIFIDVGGHLGETVEAVIDPKYGFDVIYSFEPVSSCCERIRQ
                     IRDSRVRVMTVGLSNRDFEASIHNPGSLGASLYEDAPDAQGKSEKCRFIEAARFFREH
                     IKQSDRVYLKLNCEGSEVDVIENLIINDEWSKVTNAMIDFDARKIPSQRLRVESVTNL
                     LVKTGMDNYQFPEDYMYGTGSHFGGIKNWLNKTGAIDFGIRSRLMSLIFHSKNVVGRK
                     HLGFYKLKILRHTPVFLVNYYYNKMKRNVR"
BASE COUNT          604 a          722 c          800 g          687 t
ORIGIN      
        1 gctgatcggg atcctcttcc cgctagtggc acacaggatc gcatgggtca ggtgggtctc
       61 gctggcagct ctctgctctc ttgcgctgct gctcccatta aacatttttc tcttccacta
      121 tctgttcttg ttcgcgctag ggattctggc gttcttctgt agcagcggga aaattggaag
      181 gcgcaccttc gtcttggctc tcccggcctt ctgcgccggc ctctacctga caatgggcgc
      241 ggcgatcgcc gcagtcgggg cggcggtcgc cgcgtcgatc gtcttcgtgc cgttaaataa
      301 ctcattcttg aatttcttcg gagccatctc ttactcactt tatctcttgc acgtgccgct
      361 gggcggtaaa gtgataaatc tcggggcccg cttcgagggg agctcactga gtaagattct
      421 cactctgcta gccgcttttg gtctgagcgt cggggcggcc tacctgttct acaggtttgt
      481 agagcggccg tcccagcgct ggtgctcgtc aatctcctac aggcgcgcgg ctaagccaga
      541 ggaatcatat agccctacgg ccgccacctc actatgtacc ccggtggggg tggcagagtg
      601 aaggctagcg tggatgagcg gctcgcgcct ctgtgttatg taactaagcc tgggccctcg
      661 gaactcggcg gcgagtatgc agaagtaggc ctgcgcggta agcggctgcg actagccatc
      721 acggctgacc ctgaaatagc tgtgccccca accctctacg gcggcatcga gcgcatcatc
      781 gacatgctcg tgcggggctt ggtatcccgc ggtcatgacg tgacactttt cgcgcatccc
      841 gactccgtcg ttccttgccc tgtcgtggct tatccgtgga cgacattcaa gtcgagaacc
      901 ggtacgctcc ggaacactct gctcgtctcg acgaacatcc tgcgcgggcg attcgatata
      961 gtccacagct tcgggcggtt ggcatacatg atgcctattt taccgatctc tggccctaag
     1021 atcatgagct accagaggga ggtcacggaa cgtagtgtga tatggggcac ccggctgtcg
     1081 cgtgggacgc ttcacttcac cggctgtagt cgccatctgg tccagcagtt tctggggaac
     1141 agcaactgtc acgtcgtcta taacggtgtc ccggcagata cctataagct cagggagtct
     1201 gttagcgtcg atgcgccact ggtcttcctc ggccgtgtcg aggaaatcaa aggcccacac
     1261 ctcgccgttg aaatcgcccg acgcagtggc cgctcgctcg tcattgcggg gaatatccct
     1321 gagggtgaag cccatcaggc gtttttcaaa acacatgttg caccgtacgt gaacggggag
     1381 caggtgcgat atgtagggcc agttaacgat gcagagaaga atgagctgct cggtaaagcc
     1441 gcagcctttc tgatgcctat cctatgggag gagccgttcg gcattgttat ggctgaagct
     1501 ttggcctgcg ggacacccgt cataggattg aacaggggct ccgtgccaga gatcgtgaag
     1561 cacggagtca atgggttcgt gtgcggctcg attgacgaga tgatcgctgc agtcggaagg
     1621 cttaatgaaa tagatcgcgc cgcttgccgc cgcatcatgg aagagaagtt tagcgacatg
     1681 gctgtggttg atgcgtacga aaacttgtac ttcaaattat tgggcacggc tcatccgtat
     1741 gcgtaatcgc ggcatggcaa aaatctttat tgatgtcgga ggtcacctgg gcgagactgt
     1801 cgaagctgtt attgatccca agtatggctt tgatgttatt tactcgttcg aaccggtgag
     1861 ttcatgctgc gagagaatca gacagattcg tgattcgcgt gtgcgcgtaa tgacggtggg
     1921 gctgtcaaat cgagactttg aggctagcat tcacaacccc ggctcgctag gggcatcatt
     1981 gtacgaggac gctccagacg cgcagggcaa aagtgagaaa tgtcgattca tcgaggccgc
     2041 ccgctttttc agggagcaca taaaacaatc cgaccgtgtt tacctaaagc ttaattgtga
     2101 agggtctgaa gttgatgtga tcgagaacct cattattaat gatgagtgga gtaaggttac
     2161 aaacgcgatg atcgatttcg acgcacgcaa aattccatcg cagcgtctac gggtcgagtc
     2221 cgtgactaac ttgcttgtta agaccggcat ggataattac cagtttccgg aagattacat
     2281 gtatgggact ggctcgcact tcggaggtat taagaattgg ctcaacaaga cgggcgcgat
     2341 cgattttgga attaggagtc gcctcatgag tcttattttc cattcaaaaa atgtcgttgg
     2401 caggaagcac ctagggttct acaagctgaa aatactacgt cacactccag tatttctcgt
     2461 taactactat tacaacaaaa tgaagcggaa cgttcgctaa gagaatcttt cctgaagcat
     2521 ggccgtagtt tgtttattaa cccctggtca gccttccacc aacccgcggc tcgtcaaaga
     2581 ggcggacgcg cttgttgaga ttgggcatca ggtgcacgta gtctgcgctc attgggcagc
     2641 atgggcggac gagacggaca agcttctttt gaagtcgcgg aaatttgatt gcacatatgt
     2701 cggtgggcac ccgaaggaag agtcttccaa gtaccagttg acgcgattaa gacatcgctt
     2761 ggggagggaa ttaatgcggt ttcctgtccc aatatatccg ccgaaatggg cct
//
LOCUS       DASWNS010000198        15678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_397181, whole genome shotgun sequence.
ACCESSION   DASWNS010000198 DASWNS010000000
VERSION     DASWNS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15678
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397181"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /gene="pstB"
                     /locus_tag="VGB76_06650"
     CDS             <1..768
                     /gene="pstB"
                     /locus_tag="VGB76_06650"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY0098617.1"
                     /translation="PKTTPTKISVRNLNFFYGTAQALFNISLDIPERIVMAFIGPSGC
                     GKSTFLRTLNRMNDTIPGARAVGQVLIDGEDVNAPGQNVVDLRKKVGMVFQKSNPFPK
                     SIFDNVAYGLRINRLTDNKSEMEDRVREALEDAALWSEVKDRLKTSALGLSGGQQQRL
                     CIARALAVRPEVVLMDEPASALDPIATQKIEELIVELKRQYTIVIVTHNMQQAARVSD
                     TTAFFWLGKLIEVNNTEKMFTNPNEKLTEDYITGRFG"
     gene            839..1498
                     /gene="phoU"
                     /locus_tag="VGB76_06655"
     CDS             839..1498
                     /gene="phoU"
                     /locus_tag="VGB76_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY0098618.1"
                     /translation="MDHHRRLDAELNALRDRVLLLGGEAETALLRAMYALTERDTEAA
                     REVLANDDAIDRLEVEIDRLCIDVLALRQPAARDLRFVISVAKITPILERVADHACNI
                     ARAAIDLNDEPQLKPYFDLPRMAEHASKMLHAALDSFTSGNAEEAREIIERDDAVDEM
                     YERIFHQLIELMVADPATTTRAARLLFVAKHLERVGDYVTDICELTVYMAEAAFIKHS
                     N"
     gene            1659..2384
                     /locus_tag="VGB76_06660"
     CDS             1659..2384
                     /locus_tag="VGB76_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0098619.1"
                     /translation="MSKPVLIIEDDPDIAEGLRYNLEREGLAARVAETGEQGLSFALD
                     TKNPPALILLDLMLPGMSGTELCRRLRREPLTRRTPIIMLTARVSETERVAGLELGAD
                     DYITKPFSVREVLARVRAVLRRADDATVSVYEDEKLQIHFEDVRVVCEGAEVKLTRKE
                     FALLAALARRPGRVATRQQILDDVWGQQHYGDTRTLDVHIRRLRSKLGTCADSIETLV
                     GVGYRFNGCSTLSGNRARNGSES"
     gene            2381..3712
                     /locus_tag="VGB76_06665"
     CDS             2381..3712
                     /locus_tag="VGB76_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0098620.1"
                     /translation="MTHAATPTRLKLSHALGGVLLVALPFFFMLGATAGAAVCFAALA
                     GLLLGRLSARGGQTPDTSLPSHQTLSPRALLALEAAKAAPPAGALLAATMNGMREGVL
                     AVDGSLRIVTVNDAARVLFSRAHGALSGEPLSALTRNPAIHAAYSAALERGEDNQVKV
                     ELVGAERHVLELRVAPLMLDETHATRGAIGVFFDITQLERLERVRQEFLSNVSHELRT
                     PLTAILTFVETLEEGALDDPEHSRRFVGIIGRNAARMHTLIEDILELSAIESGGTQVE
                     ARQVRLSPVVQEILSSLGGKAEARGVALVNEVRPEAVVFADPRRLEQMLLNLVDNAIK
                     FNRAGGVVTVGHEAGGASDLVTVSDTGEGIPQDQLPRIFERFYRVDHARSREMGGTGL
                     GLAIVKHLARAHGGEVCVRSAVNEGSTFTIELPRVQNTDQNTERNTERNTD"
     gene            complement(3759..4490)
                     /locus_tag="VGB76_06670"
     CDS             complement(3759..4490)
                     /locus_tag="VGB76_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0098621.1"
                     /translation="MTRRTIVIGDIHGCFDELTELLEKAALGEDDRVVSVGDLVTKGE
                     KNREVLDLFIADARFEAVLGNHDLALLRYWRGATHTLKAAQRKAVAELESDRERYARY
                     LESLPLMIDLGAHLVVHAGIRPGVPLDAQTPDDLTALRTLGADRTNRLGTPWYEVYRG
                     EKTILFGHWPDSAPRRAPCAIGLDTGCVYGYQLTAYIIETGDFISVRARRAYSAPKGQ
                     RGSHASPATTLWKRLLQGLTRQLRQ"
     gene            complement(4495..6672)
                     /gene="ppk1"
                     /locus_tag="VGB76_06675"
     CDS             complement(4495..6672)
                     /gene="ppk1"
                     /locus_tag="VGB76_06675"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY0098622.1"
                     /translation="MMAKSGKKFKQDGARLLKTSKKKKGAGAAQSLVPAEMLFNRELS
                     WLEFNRRVLEEALDESHPLLERLKFLSIFSSNLDEFFMIRVSGLKEELEEEIVELSPD
                     GMTPAEQLREISARVRPLIELQMRCLQGVILPQLASHGIVITSYNDLSKRERQAADAY
                     FNESVFPILTPQAVDPGHPFPYISNLSLNLGLMVEPGAEHGEAETPGLKGKRFARIKL
                     PPIVPRLVPVDETGRKFTFLGSLIAANAGALFPEMQHGKSHLFRVTRDADIEIREDEA
                     GDLLRSMQQQLRRRRFGSAVRLEVSATMPAKMVGYLTTSLDLTPEDVYVVDGILNVPD
                     LMQLYGLDLPALRDKPLPVSVPAPLRQGGSVFNAVKRQDVLLHHPYTSYTSVTDFIAM
                     AASDPDVLALKMCLYRTGKDSPVVQSLMEASAQGKQVTALVELKARFDEENNIEWARR
                     LEQEGVHVIYGLMGLKTHSKLALVVRREGTKLRRYVHLATGNYNPTTSRAYTDIGLFT
                     ADPEIGEDAANLFNYLTGYSRYSKYHCLLVAPVNLRARLLAFIEREAEHARAGRRSGI
                     IAKINSLTDVQIIRALYAAAQAGVPVDLIVRGVCMLRPGVPGVSENIRVRSIVGRFLE
                     HSRVLYFINDGQDEVYIGSADLMHRNLDRRVEVMAPIKDERLKKYLRDVLLESYLRDD
                     VKARTLQPDGTYQRVTPSPDGGGFNSQNEVLWTTDNPPLLKGA"
     gene            6865..7680
                     /locus_tag="VGB76_06680"
     CDS             6865..7680
                     /locus_tag="VGB76_06680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0098623.1"
                     /translation="MQYNLTERIREHYDIASVYYEKLWGKHLHHGYYLTGKESKEEAA
                     ENLIKFLVELAEIPRGARVLDVGCGIGGASAWLAEQLDCQVTGITISPGQIRMATEAT
                     RHLINRPTFLWDDANRLSVEGDFDIVWAVEVISHLNDRSEFFRRMSRLLVSGGRFCEA
                     AWLKEEELSREVEDKYILPIEEGMLVSLPTLSEYKRHIEENRLRLLHYEDISSKVAQT
                     WDICLDIARDRAVWEFAAQHGREFIAFLKSFKAMRNGFKTGTFRYGVLVVEKP"
     gene            complement(7881..8303)
                     /locus_tag="VGB76_06685"
     CDS             complement(7881..8303)
                     /locus_tag="VGB76_06685"
                     /inference="COORDINATES: protein motif:HMM:NF039723.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098624.1"
                     /translation="MVNRKVTREAGVQTVSSTDDTTVVIGAPVVIKLRDKKRKKRGSS
                     KAARRLEDYEKRMSKSARRVSRGVKNGVDTYIDNRNRSERKRRDGALVDFCENAAKGI
                     ARAISESSPVMTDYAKAMNTKQLRKQIRQFLRPIPVIA"
     gene            8556..9851
                     /locus_tag="VGB76_06690"
     CDS             8556..9851
                     /locus_tag="VGB76_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0098625.1"
                     /translation="MPDLKILLIQISLVLITCYVVSWVLGKVQQPRVVGEMVAGILLG
                     PSLLGWLAPGLSAAIFPPESLGLLSALSQVGLLLFMFMVGLELDTRNLRQLGHTAIVI
                     SHTSIVVPFVLGGLLAYYLYPRVADETLPFTGFMLFMGAAMSVTAFPVLARILTERNL
                     LTSRLGTLTITCAAVDDVTAWCILAAITGIVRAQHAQLTLWEMLAGLVVYFVAMLFVL
                     RPIIRRLANKLEGAEAGAADKILVVLLLTVLASSWATEWLGIHALFGAFFAGVIMPKQ
                     EGFTEDIRKKLRPLVVVLLLPLFFAFTGLRTSIGLIIAPSMLFYCGLVFLVAVAGKWG
                     GSMLAARVMGTPWREAAAIGILMNTRGLMELVILNIGMDIGVLTPPLFSMMVVMAVGT
                     TLMTTPFLSLLYPETKNQALPQRQEAIGYQSFETPQSGD"
     gene            10386..11126
                     /locus_tag="VGB76_06695"
     CDS             10386..11126
                     /locus_tag="VGB76_06695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0098626.1"
                     /translation="MAGFINDNGIVNPLNRGAFYACRNEQDQIEGIALIGHLTLFEAR
                     TDAALEAFAQHARSCPHIYTITGEQEKLERFWEHYATDGRLPRQLSCEFLYVQHEFVE
                     MPSVGDVRSARPDDIEVLLPLYAMMVFEASGINPLETDPLGFRLRLVRRVEQGRVLVW
                     IEQQEVLFTASIISDTPTANYLEGVYVNPAHRGKGYGLRCMSQVSKKLLARTRSICGF
                     VNEQNSVAQAFYRKAGYELDSCYKKIYV"
     gene            11328..13772
                     /locus_tag="VGB76_06700"
     CDS             11328..13772
                     /locus_tag="VGB76_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0098627.1"
                     /translation="MRFLVLAVVTVCFGSRIGIEVSRLKFKITISDTFIFVALLLYGG
                     ELAILLAGAEALCTSLRSRWKVSTILFNTGLLAFSTFITVSALNFLFGSIVNLSHGDI
                     SAKFAIAICVMAVVQCLANSVPAALREALKLNQSFLHLWKQFYFWTCITYFAGASAAG
                     ITVKLIAVGGLYAFVLTIPLISIIYFTFKTYSKHIAATTAQAEQAIQHVEELNHHIIE
                     QNRISQALQESEEHFRSAFNYAAIGMALMATDGRWLQVNNSLCQIVGYTEPELLAMNA
                     QAITHPEDLGKHLTDVYMLLEGQIVTSSMEKRYLHKLGFPVWVMSSASLVRNALGEPL
                     HFIVQMQDITERKHAEEQLHHAAFYDGMTGLPNRALFTDQLQLTIERVKQAPDQMFAV
                     LFLDVDRFKNINDSLGHVFGDQLLSTIARRLEKCVRPEDTVARFGGDEFAILLNSIKH
                     SVDAIRVADRVQAELAQSFSLCGQEIFTTASIGIALSSTGYNHPEELLRDADTAMYRA
                     KAQGKGRHEVFDKVMHARAISLLQLENDLRRAIEREEFIIHYQPIVKLATGLISGFEA
                     LVRWQHPERGLVSPLEFIPLAEETELIIPISHWVLRESCRQIRQWQLESPAMASLSIS
                     VNLSGKQFKQSGLVEYIKQTLREANLAPAALRLEITESVVMENADITTAMLRQLRSLG
                     VQLSIDDFGTGYSSLSYLHRLPVNNLKIDRSFVSQMRPGNENSEIVRTIITLARNLNM
                     EVVAEGIETEDQLGQLKALACDYGQGYLLSKPVTAEAARTLLHSNFAELANSFMAGAA
                     IAQEEEDKILTSTLSM"
     gene            complement(14079..>15678)
                     /locus_tag="VGB76_06705"
     CDS             complement(14079..>15678)
                     /locus_tag="VGB76_06705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY0098628.1"
                     /translation="KGVTIAVMDSGISKTHADFSKNKNKSRILTAVDFTGEDSTGDIN
                     GHGTGVASIAAGNGSATNYSGTKSVGVAPDADLVDLRVLDANGAGKTSSVLRAINWAI
                     VNRQRYNIRVLNMSIGSPVRESFRLDPVCLAVADAVRAGIVVVASAGNTGRTDETDAN
                     GNVVHRPLYGSIKSPGNSPYVITVGAADSRGTAKRSDDIMAEWSSKGPTQYDRSAKPD
                     IVAPGRHLIAAIDPNSTLATNNADRVQNSPDNNYFKFSGTSFSAPVVSGTIALMLEAN
                     KSLNPTLVKAALMRTAQTVPGENFTSVQQRLFTQGTGLVNAVGAVKMARAIVPNADKL
                     VAGDKIFSSNESLTSLGDSVQVAGETIPLSGNLLYSNGIIFRDEPILTNGILLGDGIV
                     VSDGLILSDGIVVSDGIMISDGIMISDGIVVSDGIVVSDGIMISDGIMISDGIVVSDA
                     LLGDGIVVSDGIVVSDGIVVSDGIVVSDGIVVSDGIMISDGILASDGIVVSDGIVVSD
                     GIMISDGIVVSDGIVVSDGIVVSD"
BASE COUNT         3202 a         4628 c         4538 g         3310 t
ORIGIN      
        1 cccaagacca cgccgaccaa gatcagcgtg cgcaacctga attttttcta cggcaccgcg
       61 caagccctct tcaacatctc gctcgacatt ccggagcgca tcgtgatggc ctttatcggt
      121 ccttccggtt gcggcaagtc aaccttcctg cgcacgctca atcgcatgaa cgatacgatt
      181 cccggcgcgc gtgccgtggg tcaggtcttg atcgacggcg aagacgttaa cgcgccgggg
      241 cagaacgtcg tggatttgcg caagaaggtg gggatggtct ttcagaagtc gaacccgttt
      301 ccgaaatcca tcttcgataa cgtcgcctac gggttgcgta tcaaccgcct gacggataac
      361 aagagcgaga tggaggatcg cgtccgcgaa gcgttggaag acgcggcctt gtggtcggag
      421 gtgaaggatc gcctgaagac ttccgcgctc ggcctctccg gcgggcagca gcagaggctc
      481 tgcatcgcgc gcgctttagc cgtgcgcccc gaagtggtct tgatggacga accggcctcc
      541 gccctcgacc ccatcgcgac gcagaagatc gaagagttga tcgtggaact caagcggcag
      601 tacaccatcg tcatcgtgac gcacaacatg cagcaggcgg cgcgcgtctc cgacaccacg
      661 gctttcttct ggctcggaaa actgatcgaa gtcaacaaca cggagaagat gttcactaac
      721 ccgaacgaga agttgaccga agactacatc acggggcggt tcggctgatg ctccttgtgc
      781 gggcttcgac agccgcgcga aaaactgacg cgaaagatga caaggggagg tttttaaaat
      841 ggatcaccac agacggctcg atgccgaact aaacgctctg cgcgaccgcg tgctgctcct
      901 cggcggcgag gccgagacgg ctctgctgcg cgccatgtac gcgctcacgg agcgagacac
      961 ggaggcggcg cgcgaggtgc tcgccaacga cgatgcgatt gaccggctcg aagtggagat
     1021 cgaccgtctc tgcatagatg tgcttgcgct gcgccagccc gccgcgcgcg atctcaggtt
     1081 tgtcatttcg gtcgctaaga tcacgccgat cctcgaacgc gtggcagacc acgcctgcaa
     1141 catcgcccgc gccgccatcg atctcaacga cgaaccgcaa ctcaaaccct atttcgacct
     1201 gccgcgcatg gccgaacacg cctcgaaaat gcttcatgcc gcgctcgact ccttcacgag
     1261 cggcaacgcc gaggaggcgc gcgagatcat cgagcgcgac gacgcggttg acgagatgta
     1321 cgaacgcatc tttcaccaat tgatcgaatt gatggtcgcc gatccggcga cgacgacgcg
     1381 cgccgcgcgc ctgctcttcg tcgccaaaca cctcgaacgc gtcggcgact acgtgacgga
     1441 catctgcgaa ctcacggtct acatggccga ggctgctttc atcaaacaca gcaactaaga
     1501 gaagtgacaa gtgacaggtg acgagtgaca agttaaaaag acgtaaatcg taaatcgtca
     1561 acagagaaaa tttgtctatc cttatctatt tacgatttac tgtctccatc tcgtcacttg
     1621 tcactcgtca ctgttcttgg cactcgtcac ttaaaaatat gtcaaagcca gttctcatca
     1681 tcgaagacga cccggacatc gcggagggct tgcgttacaa ccttgagcgc gaagggctgg
     1741 cggcgcgcgt agcggagacg ggtgagcaag ggctttcctt cgcgctcgac acgaagaacc
     1801 cgcccgcgct gatcctgctc gacctgatgc tgcccggcat gagcgggacg gagttgtgcc
     1861 gccgcctgcg ccgcgaaccg ttaacgcgcc gcacacccat catcatgctg acggcgcgcg
     1921 tttcggaaac cgagcgcgtg gcgggtctgg aactcggcgc ggacgattac atcaccaagc
     1981 cgttttcggt gcgtgaagtg ctggcgcgcg tgcgcgccgt gctgcggcgg gcagacgatg
     2041 ccaccgtctc cgtctacgaa gacgaaaaac tgcaaatcca tttcgaggat gtgcgcgtcg
     2101 tctgcgaggg agcggaggtg aaactgacgc gcaaggagtt tgcgctgctc gccgcgctcg
     2161 cgcgtcgtcc ggggcgcgtg gcgacgcgtc aacaaatcct cgacgacgtg tggggtcaac
     2221 aacattacgg cgacacgcgc acgctcgacg tgcacatccg gcggctgcgc tccaagctcg
     2281 gaacgtgcgc cgacagcatc gagacgctcg tgggcgtcgg ctatcgcttc aacggatgct
     2341 cgacgctctc cggcaaccgc gcccgtaacg gatcggagtc ttgacgcacg ccgccacgcc
     2401 aacacgcctt aaactctcgc acgcgctcgg cggcgtcctg ctcgtcgcgc tccccttctt
     2461 tttcatgctc ggcgcaacgg caggcgcggc ggtctgcttt gccgcgctcg caggtctgct
     2521 cctcggtcgt ctctcggcgc gcggcgggca gacgccggac acttccctcc cttcgcatca
     2581 gacgctctcg ccacgcgcgc tccttgcact ggaagcggct aaagccgcgc cgcccgcagg
     2641 cgcgctgctc gccgcgacga tgaacgggat gcgcgaaggc gtgctggccg ttgacggctc
     2701 gctgcgcatc gtgacggtga acgacgcggc gcgcgtgctc tttagtcgcg cacacggcgc
     2761 gctctccggc gagccgctca gcgcgctgac gcgcaacccc gcgattcacg ccgcctacag
     2821 cgcggcactc gaacggggcg aggacaatca agtcaaagtc gagttggtcg gcgcggagcg
     2881 gcacgtcctt gagttgcgcg tcgcgccgct catgctcgac gagacgcacg ccacgcgcgg
     2941 cgcaatcgga gtcttcttcg acatcacgca actggaacgg ctggagcgcg tgcggcagga
     3001 attcctctct aacgtctcgc acgaattacg cacgccgctc accgccatcc tcaccttcgt
     3061 cgagacgctc gaagagggcg cgctcgacga cccggagcac agccgccgct tcgtaggcat
     3121 catcggtcgt aacgccgcac gcatgcacac gctcattgaa gacatccttg aattgtcggc
     3181 catcgagtcg ggcggaactc aggtggaagc gcggcaggtg cggctgtcgc cggtggtgca
     3241 ggagattttg agttcgctcg gaggaaaagc cgaagcgcgg ggcgttgcgc tcgtcaacga
     3301 agtcaggccg gaggcggtcg ttttcgcaga cccgcgacgg ctcgaacaga tgctcctcaa
     3361 tctcgtggac aacgcgatca agttcaaccg tgccgggggc gtggtcacgg tcgggcacga
     3421 ggcgggcggc gcgagcgatc tcgtcaccgt cagcgacacg ggcgaaggca tcccgcaaga
     3481 tcaactgccg cgcatcttcg aacgcttcta ccgggttgac cacgcgcgtt cacgcgagat
     3541 ggggggcacg gggctggggc ttgccatcgt caagcatctg gcgcgggcgc acggcggcga
     3601 agtgtgcgtc aggtcggcgg tcaacgaagg ctcgaccttc accatcgaac tgccccgcgt
     3661 tcagaacaca gaccaaaaca cggagcggaa cacagagcgg aacacagact gacgcaggcg
     3721 gtcgcgcctt cttcgtccgg cgttcacggc gtcgccgttc attggcgaag ctggcgcgtc
     3781 aaaccttgca gcaggcgttt ccagagagtt gtcgcaggcg acgcgtgtga gccgcgctgc
     3841 cccttaggcg cggagtaagc cctgcgcgcc ctgacgctga taaagtcgcc cgtctcgatg
     3901 atgtaggcgg tgagttggta gccgtagacg cagcccgtgt cgagtccgat ggcgcagggt
     3961 gcgcgccggg gtgcgctgtc aggccagtgt ccgaagagga tcgtcttctc gcccctgtag
     4021 acctcatacc acggcgtgcc caagcggttc gtgcgatccg cgccgagcgt gcgcagcgcg
     4081 gtcagatcgt cgggcgtctg cgcgtcgagc ggcacgccgg ggcgtatgcc cgcgtgcact
     4141 acgaggtgcg cgccgaggtc tatcatcaga ggtaaggatt cgaggtagcg cgcgtagcgt
     4201 tcccggtcgc tttcgagttc tgcgacggcc tttcgctgcg ccgccttgag cgtgtgcgtc
     4261 gcgccgcgcc agtagcgcag caacgccaga tcgtgattgc cgagcaccgc ctcgaaacgc
     4321 gcgtccgcga tgaagaggtc gagcacttcg cggttcttct cgcccttcgt cacgaggtcg
     4381 ccgacggaga cgactcgatc atcttcgccg agcgcagcct tttccagtag ctccgtgagt
     4441 tcgtcgaagc aaccgtgtat gtcgccgata acaatcgtgc gccgcgtcac tgcttcatgc
     4501 cccctttaac agcggagggt tatcggtcgt ccagagaact tcgttctggc tgttgaatcc
     4561 cccgccgtca ggcgaaggcg tgacgcgctg gtaagtgccg tccggctgca aggtgcgcgc
     4621 cttcacgtcg tcgcgcaggt aggactccag caagacatct ctcaggtatt tcttgagccg
     4681 ctcatctttg atcggagcca tcacctcgac gcgtcgatca aggttgcggt gcatcaagtc
     4741 cgcgctcccg atgtagacct cgtcctgccc gtcgttgatg aagtagagca cccggctgtg
     4801 ttcgaggaag cgacccacga tgctcctgac acggatgttt tccgaaacgc ccggcacgcc
     4861 cggacgcaac atgcagactc cgcgcacgat caaatccacg ggcacgcctg cctgcgcggc
     4921 ggcgtagagc gcgcggatga tctgcacgtc ggtcagactg ttgatcttgg cgatgatgcc
     4981 cgaccgacga ccagcgcggg cgtgctcggc ctctcgctcg atgaaggcca gcaggcgcgc
     5041 acgcaggttg accggcgcga cgagcaggca gtgatatttg gaatagcgcg agtagccggt
     5101 caggtagttg aagaggttgg cggcgtcttc gccgatctcc gggtcggcgg tgaagaggcc
     5161 gatgtccgtg taggcgcgcg aggtcgtggg gttgtagttg cctgtggcga ggtggacata
     5221 acgcctgagc ttcgtgcctt cgcggcgcac gacgagcgcg agtttgctgt gcgtcttcaa
     5281 gcccatcagg ccatagatca cgtggacgcc ctcctgctcc aaacgccgcg cccattcgat
     5341 gttgttctcc tcgtcgaagc gcgctttgag ttcgacgagc gcggtcacct gtttgccctg
     5401 tgcgctcgcc tccatgagcg actgcacgac gggcgagtct ttgcccgtgc gatagaggca
     5461 catcttgagg gcgagcacat cggggtcgct cgccgccatc gcaatgaagt cggtgacgga
     5521 cgtgtaagaa gtgtaggggt gatggagcag cacgtcctga cgcttgacgg cgttgaagac
     5581 ggagccgccc tgccggagcg gtgcggggac agacaccgga agcggcttgt ctctgagcgc
     5641 gggcaggtcc agcccgtaaa gctgcatcag gtctgggacg ttcaatatac cgtcaacgac
     5701 gtacacgtct tcgggcgtca ggtcgagaga ggtcgtcagg tacccgacca tcttggcggg
     5761 catcgtggcg gagacttcga gccgcaccgc cgagccgaag cggcgacggc gcaactgctg
     5821 ctgcatggag cgcaggaggt cgccggcctc gtcctcgcgg atttcgatgt cggcatcgcg
     5881 cgtcacgcga aacaggtgcg acttgccgtg ttgcatctca gggaagagcg cgcccgcgtt
     5941 cgccgcgatc aggcttccga ggaaggtaaa cttcctgccc gtctcgtcaa ccggcacgag
     6001 gcgcgggacg attggcggca gcttgatgcg tgcaaaccgc tttcccttca gacccggagt
     6061 ttccgcctcc ccgtgctctg cccccggctc aaccatcaat ccgagattga gactcaggtt
     6121 ggagatgtag gggaatgggt ggccggggtc tacggcctgc ggggtgagaa tggggaagac
     6181 gctttcgttg aagtaagcgt cggcggcctg cctttcgcgt ttgctcaggt cgttgtaaga
     6241 cgtgatcacg atgccgtgcg aggcaagctg cggcagaatc acgccctgca agcagcgcat
     6301 ctgcaactcg atcaaaggtc gcacccgcgc gctgatctca cgcaactgtt cggcaggcgt
     6361 catgccgtcg ggcgacaact cgacgatctc ctcctccaac tcttctttca gacccgaaac
     6421 gcgaatcatg aaaaactcgt cgaggttcga tgagaagatc gagagaaact tgaggcgttc
     6481 gagcagcgga tgcgactcgt cgagagcctc ttcgagcacg cggcggttaa attccagcca
     6541 actcaactcg cggttgaaca acatctccgc agggacgagc gactgcgccg cgcccgcccc
     6601 cttctttttc ttggaggtct tcaacaggcg cgctccgtcc tgcttaaatt tcttgcccga
     6661 cttagccatc atccaacact tcccgaaagt aaaaatcgtc ttcaaccaat ttgattcggc
     6721 aacctttata gcacaggaaa gcgcgtctat aacgcgtccg tggcgtcctc gatttcaggc
     6781 atgacggttg acatactgcg ttaaattatc catcttgcct caagtaaaaa acggcctctg
     6841 ccgtgaacgc tcataacgac cattatgcaa tacaatttaa cggagagaat tcgagagcat
     6901 tacgatattg cgtctgtcta ttatgagaag ctgtggggca agcacctcca tcacggctat
     6961 tacctcaccg gcaaagaatc caaagaagag gctgccgaga acctgatcaa atttctggtc
     7021 gaactggcgg agataccgcg cggcgcgcgc gtgttggatg tcggctgcgg gatcgggggc
     7081 gcgtccgcct ggctggccga gcaactggac tgccaggtca caggcatcac gatcagcccc
     7141 ggccaaattc gtatggctac agaggccaca cgccatttga taaacaggcc gacttttttg
     7201 tgggacgacg ctaaccggct gagcgtcgag ggagacttcg acatcgtgtg ggcggtggag
     7261 gtgatttctc atctcaacga ccgcagcgag ttcttccgcc ggatgagtcg tcttctggtt
     7321 tccggcggga ggttctgcga agccgcgtgg ttgaaggaag aggaattgag ccgcgaggtt
     7381 gaggataagt atatcctgcc gatagaagag gggatgttag tctcgctccc cactctgtca
     7441 gagtataaaa ggcatatcga ggagaacaga ctgcgcctgc tccactacga ggacattagt
     7501 tcgaaagtgg ctcagacgtg ggatatatgt ctagacatcg cgagggatcg agccgtgtgg
     7561 gaattcgccg ctcaacacgg cagagagttt atcgccttcc tcaagtcctt caaggcgatg
     7621 aggaacggct ttaagaccgg cactttcagg tatggcgttc tggtggtcga aaaaccctga
     7681 ggacgtgttt caaaggcgaa gggcaataat atacttcacc caccagaagg ctcttgcggc
     7741 catagacgct tcagaaataa aaaggtctta cgaggtaagc cgggcactgg tgagggaaat
     7801 atattaccgc cctgaattat gaggtcgagc aattggcaaa ccgccggacc ccccctaccg
     7861 caaggctctc cggctttcag ctacgcgatt acgggaatcg gacgcaaaaa ttgcctgatc
     7921 tgctttctca actgtttggt gttcatcgct ttggcgtaat cggtcatgac ggggctggat
     7981 tcggagatcg ctctggcgat gcctttcgcc gcgttctcac aaaaatctac cagtgcgccg
     8041 tcgcgtctct tacgctctga acgattgcga ttatcgatgt aagtatcgac accgtttttg
     8101 acgccccggc tgacgcgacg cgccgatttg ctcatgcgtt tctcgtagtc ttcgagacgc
     8161 ctcgcggctt tcgaagagcc acgcttcttt ctcttcttat ctctcaactt aataacgacg
     8221 ggcgcgccaa ttacgacggt cgtgtcgtcg gtcgaactaa cggtctggac tccagcctct
     8281 cttgtcacct ttctattcac catttttttt ggtgacctcc tattctcgga tttaattccg
     8341 gaagatacac gccttatact ccgctgccag attactgtca agatttatca ttgccaaaaa
     8401 cgcgaagttg ttgcataaat actggcaagc agttgttcac gaatttgcag gctatcctgt
     8461 tagcggctga tcgtagagtg aactgtatta tcgcctcacg aatctcgctg tttaacccta
     8521 tttagcaaca cgatctccca tgtgagggta aagcgatgcc cgacctcaag attttattga
     8581 ttcaaatctc ccttgtcctt atcacgtgct atgtcgtcag ttgggtgtta ggcaaggttc
     8641 agcagccgcg tgtggtcggt gaaatggtcg cggggattct gctcggcccc tctctgctcg
     8701 gctggctcgc gcccggtttg tcggcagcca tattcccgcc ggagagcctg ggacttttga
     8761 gtgcgctcag tcaggttggc ctgctgctct tcatgttcat ggtcgggctg gaactcgata
     8821 cgcggaatct gcgtcagttg gggcacacgg ctatcgtcat tagccatacg agcattgtcg
     8881 tgccgtttgt gctgggcggg ttgctcgcct attaccttta cccgcgcgtg gcggacgaga
     8941 cgctgccctt cacaggattc atgttgttca tgggagcggc catgagcgtc acggccttcc
     9001 ccgtgctggc gcgcatctta accgaacgaa atctgctcac ctcaagacta gggacgctga
     9061 ccattacctg cgcagcggtg gacgacgtaa ccgcctggtg cattctggca gctatcacgg
     9121 gcatcgtcag agcacaacac gcccaactca cgctatggga gatgctggcc gggctggtcg
     9181 tatatttcgt cgccatgctg ttcgtgctcc gacccataat tagacgcctc gcaaacaaac
     9241 ttgagggcgc ggaagcaggc gcagccgaca aaatactcgt cgttctactc ctgaccgtgc
     9301 tcgcctcaag ctgggcgacg gaatggctcg gcatccatgc gcttttcgga gccttcttcg
     9361 ccggtgtcat catgccgaag caggagggtt tcaccgaaga cattcgaaaa aaactccgtc
     9421 cactcgtcgt cgtccttctc ttgcctctgt tttttgcctt cacaggactg cgcaccagta
     9481 tcggtctgat catcgcgccc tcgatgctct tctactgcgg gctggtcttt ctggttgcgg
     9541 tcgcgggcaa atggggaggg tcgatgctcg cggcgagggt catgggcacg ccctggcgtg
     9601 aagcggcggc catcggaatc ctgatgaaca cgcgcgggtt gatggagctg gtaattttaa
     9661 atattggcat ggacatcggt gtcctcaccc cacctctatt ctcaatgatg gtagtgatgg
     9721 cggttggcac gaccctgatg acgacgcctt ttttgtctct gctctatccg gagactaaaa
     9781 atcaagctct gccacaaagg caggaggcta tcggttacca gagtttcgag acgcctcagt
     9841 ccggtgattg acgccctctc gtctccatcg aagcccgaag ttggaacgcc aattgaggaa
     9901 acctacctga gcgcttcccg taagtgcaaa acggtgttat catccggggt ggaaagcctc
     9961 tccctgaagg ctgcccacat tgcaagggtt aaaacagcgt tcccggcctt cgacttgccc
    10021 aagcacattc tcaccccgtg tttttaagtg atctctcttc caatcgaatt gagtgaccga
    10081 aacgtttcag gctgaccgac ttctgctttg agagtcgtct cacgttttct cgttaacctg
    10141 ataagctcac tggactcaat cagttctgaa attttgcgca tctggtatgc tgattgcaaa
    10201 tgtgatgatt gcccgcatcg cgtcattcgc gcgaacgtct cgcatcagaa aaacccgaca
    10261 aattgcagta gagctatcgc gtaccttcta gcgcgaatat ccttatgact cttcaagaac
    10321 tcacaaacac gcacgctgcc gagacgctcg cctttctcgc cgagcgtccg cttcacactg
    10381 taattatggc cgggtttata aacgacaacg gtatcgtgaa cccgctcaat cgcggcgcct
    10441 tctatgcctg ccgtaatgaa caggatcaaa ttgagggcat cgccttaatc ggtcatctga
    10501 cgcttttcga ggcgcgcacg gacgccgccc tagaggcttt cgctcaacac gcgcgttcct
    10561 gtccgcatat ctacacgatc acaggtgaac aggagaagct cgaacgattc tgggaacatt
    10621 acgcgacgga cggacgattg ccgcgtcaat taagttgtga gttcctgtat gtgcagcatg
    10681 agttcgtaga aatgccttcg gtgggagatg tgcggtcagc ccgccccgac gatattgaag
    10741 tgttgctgcc tctatatgcc atgatggtct ttgaagccag cgggatcaat ccgctggaga
    10801 cagacccact cggttttcgc ctccgactcg tgcggcgtgt cgagcaaggc agagtgctcg
    10861 tctggatcga acagcaggaa gtgctcttca ccgccagcat catctctgac actccgacgg
    10921 ccaattatct cgaaggcgtc tacgtcaatc ccgcgcatcg cggcaaaggc tatggcctgc
    10981 gctgcatgtc gcaggtaagt aaaaagcttt tggcgcggac gcgttctatc tgcggattcg
    11041 ttaacgagca gaacagtgtg gcgcaagcct tctaccgcaa agccggatac gaactggata
    11101 gttgctacaa aaaaatctac gtgtgagtga gaagttgttg cgccgccctc gatgttcatc
    11161 ttcttcccga aggacgtgaa aaacaaggac tctacaaact aaccggataa acccgcacgc
    11221 cagtgaataa acaacgcctc aacaaaccgt tcatgtgctt aatcattgcc ctcggcgcac
    11281 tggtttgttg tctttctatc tatcgcctac cactgtcgat catagatatg cgttttctcg
    11341 tactggctgt cgtgaccgtc tgcttcggct cgcgcatcgg gattgaagtc tcacgtctga
    11401 aatttaagat cacgatctcg gacaccttca tcttcgtggc tttattgctg tacggcggcg
    11461 aactggcgat cctgctggcc ggggctgaag ctctgtgcac ctctctacgt tccagatgga
    11521 aagtctccac gatcttattc aatacggggc tgctggcgtt ctcgactttt atcaccgtct
    11581 cggcgttgaa tttcttattc ggctcgattg taaatttatc gcatggagat atctccgcta
    11641 agttcgctat tgccatttgt gtcatggcgg tggtgcagtg tcttgccaac tccgtgcccg
    11701 ccgcgctacg ggaagcgttg aagctcaacc agtctttcct gcacctgtgg aaacaattct
    11761 atttctggac ttgcatcaca tattttgcgg gagcttctgc ggctggcatc accgtcaaac
    11821 ttatcgccgt cggcggcctc tacgccttcg tcctgaccat cccgctcatc tcgatcatct
    11881 attttacttt taagacatac agcaaacata tcgcggcgac gacggcacag gccgaacagg
    11941 ccatccagca cgtcgaagaa cttaatcatc acatcatcga acaaaaccgc atcagtcaag
    12001 cactgcaaga gagcgaggaa cactttcgga gcgcgttcaa ctatgcggct atcggcatgg
    12061 ccttgatggc aaccgatgga cgctggctgc aagtcaataa ttccttgtgt cagatcgtgg
    12121 gctacacgga gccggagtta ctggcgatga acgcacaggc cattacgcac ccggaggact
    12181 tgggcaagca cctgacggat gtttacatgc tgctcgaagg ccagatcgtg acgagttcga
    12241 tggagaagcg ttacctgcac aaactgggat tccctgtgtg ggtcatgtcg agcgcgtcgc
    12301 tcgtgcgtaa cgcgcttggc gaaccgctgc acttcatcgt ccagatgcag gatattaccg
    12361 agcgtaaaca cgcggaagaa caactccacc acgccgcctt ctacgatgga atgacggggc
    12421 ttcccaaccg ggctttattc acagatcaat tgcagttgac gattgagcgt gtgaagcaag
    12481 cgcccgacca gatgttcgca gtgctttttc tggatgttga ccggttcaag aatatcaacg
    12541 atagcctcgg gcacgtgttt ggagatcaac tgttgagcac gattgcccgg cggctggaga
    12601 agtgcgtgcg cccggaggat acggtggcgc ggtttggcgg ggacgagttt gcgatcctcc
    12661 tcaactccat caaacactcg gttgacgcga tccgcgtggc ggatcgggtg caggcggaac
    12721 tggcgcaatc gttcagcctc tgcgggcagg aaatattcac gactgcgagc atcggcatcg
    12781 ccctctcttc gacgggttac aaccacccgg aagaactctt gcgcgacgcc gacacggcca
    12841 tgtaccgcgc gaaggcgcag ggcaaaggcc gccacgaagt ctttgataaa gtcatgcacg
    12901 cacgcgccat ctcgctgctg caacttgaaa atgatctacg acgcgctatc gaacgcgaag
    12961 agttcatcat ccactatcag ccaatcgtca aattggcgac cggcctcatc tccggcttcg
    13021 aagctctcgt gcgctggcaa catccggagc gcgggctagt ctcgccgctg gagttcatcc
    13081 ccctcgccga agagacggaa ttgatcatcc cgattagcca ttgggtgctg cgcgagtcgt
    13141 gccgccagat acggcaatgg cagttggagt cgcccgcgat ggcgtcgctc tccataagcg
    13201 ttaatctttc aggcaaacag ttcaagcaga gcggtctggt cgaatacatc aaacagacgc
    13261 tccgggaggc taatctcgcg cctgctgctt tgcgtctgga aattacagag agcgtggtga
    13321 tggagaacgc agatattaca accgcgatgc tcagacaact gagatcgctc ggcgttcaac
    13381 tgagcattga cgatttcggc acgggctatt catcgctcag ttacctgcat cgcctgccgg
    13441 tcaacaactt gaagatcgac cgttcattcg tgagccagat gcgccccggc aatgaaaact
    13501 cggagatcgt gcggacgatc atcaccctcg cgcgaaattt gaacatggaa gtggtggcgg
    13561 aaggaatcga gacggaagat caactcgggc aactcaaagc cttggcctgc gattacgggc
    13621 agggatacct gctctccaag cctgtgacgg cagaggcagc gcgcacgctg ttacacagca
    13681 acttcgccga actggcaaat tcgttcatgg ccggcgcagc tatcgcacaa gaagaagaag
    13741 acaaaatttt gactagcacc ttatccatgt aacttcggca gacgactcag atgcccctcg
    13801 ccgctcaacc tattccctcc gctccgcgcc accctttgtc ccttataaaa cagtctcggt
    13861 tgtaacgagc agatcataaa aaagcacgcg gggcggcatg ttcctgcccc gcgtgcttta
    13921 ggctcgctca actctgcgtc gtttgaatca agcaaaccaa tttcgtgatg cgatctcggt
    13981 tcatgctcca agctgacagg gaaatcacct tgtcagcttt gctttccccc ttccaggaaa
    14041 gctcatcagc cggagcaatc gccgccgtac gaaatatctt aatcgctcac gacaatccca
    14101 tcactcacga cgatgccatc actgaccacg atgccatcgc taatcatgat gccatcgctc
    14161 accacaatcc cgtcgctcac gacgattcca tcactagcca gaatcccatc gctgatcatg
    14221 atgccgtcgc tcacgacgat cccgtcactg acgacgatcc cgtcacttac aacgatgcca
    14281 tcgctcacga cgatcccgtc gcttaccacg atcccgtcac cgagtagggc gtcgctgacc
    14341 acgatgccat cgctaatcat gatgccatcg ctgatcatga tgccgtcgct cacgacaatc
    14401 ccgtcactaa cgacgatgcc atcgctgatc atgataccgt cgctgatcat gatgccgtcg
    14461 ctcacgacga tgccgtcgct caggatcaga ccgtcgctga cgacgatgcc atcgccgagc
    14521 aggatgccgt ttgtgaggat cggctcatcg cggaagatga tgccgttgga gtagagcaga
    14581 ttaccgctga gagggatggt ttcacccgcg acctgcaccg agtcgccgag ggaggtgagg
    14641 ctctcgttgc tgctgaaaat cttgtcgccc gcaaccagct tgtcggcgtt gggcacgatg
    14701 gcacgcgcca tcttgactgc gcccactgcg ttgacgagtc cggtgccctg cgtgaacagg
    14761 cgttgctgca cggacgtgaa gttctcgccg ggcacggtct gcgcggtgcg catcagagca
    14821 gctttaacga gcgtcgggtt gagcgacttg ttcgcttcca gcatcagggc gatggtgccg
    14881 gagacgaccg gcgcggagaa ggaggtgccg gaaaacttga agtaattgtt gtcggggctg
    14941 ttttgaactc tgtcggcatt attcgtcgcc agcgtggagt tcgggtcaat cgccgcgatg
    15001 agatggcgac cgggggcgac gatgtcaggc ttcgcggaac ggtcgtactg cgtcgggcct
    15061 ttggaagacc attcggccat gatgtcatcg gagcgtttgg cggtgccgcg cgaatccgcc
    15121 gcgccgaccg tgatcacgta agggctgttg ccggggcttt tgatgctgcc gtagagggga
    15181 cgatgaacga cgtttccgtt ggcgtccgtc tcgtctgtgc gaccggtgtt gcctgcgctg
    15241 gcgaccacca cgatgcccgc acgaacggcg tcggccacgg cgagacacac cgggtcgagg
    15301 cggaaagatt cgcgcacggg cgagccgatg ctcatgttga gcacgcggat gttgtagcgt
    15361 tgacgattga caatcgccca gttgatcgcg cgcagcacgc tgctcgtctt gcccgcgccg
    15421 ttggcgtcga gcacgcgcag gtcaacgagg tcggcgtcgg gcgcgacacc cacggacttc
    15481 gtgccggagt aatttgtcgc cgagccgttt ccggcggcga tgctcgccac gcccgtgccg
    15541 tgaccgttaa tgtctcccgt gctgtcctca ccagtgaaat cgacggcggt gagaatccgc
    15601 gacttgtttt tgttcttgct gaagtcagcg tgcgtcttgc tgatgccgct gtccatcacc
    15661 gcgatggtca cgcccttg
//
LOCUS       DASWNS010000199         5255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_59397, whole genome shotgun sequence.
ACCESSION   DASWNS010000199 DASWNS010000000
VERSION     DASWNS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59397"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VGB76_06710"
     CDS             <1..297
                     /locus_tag="VGB76_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY0098629.1"
                     /translation="AWETIQRGHAIANGVYVVVVNRVGAEGKLKFWGQSFVVDPFGRI
                     VAKGSADKEEVLIVECSLDNIERTRQNWPFLRDRRIESYQGLGYRFLETPSTKS"
     gene            358..1398
                     /locus_tag="VGB76_06715"
     CDS             358..1398
                     /locus_tag="VGB76_06715"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEY0098630.1"
                     /translation="MNQQQTPAALGFRMPPEWHRHAATWLTWPKDPLTWPDRVPQVEE
                     IYLQIIAALTPHELVNLLVDDEATEASVRARCKFEASERVRFHRIPTVDSWIRDYGPN
                     FLTSDDGKLAYNDWIFNAWGDKYEELKRDDAIPKRLESLLGVPRFEPGIVLEGGSIEV
                     NGAGVVLTTEQCLLNPNRNPQLERAEVETYLKNYLGVEQVLWLGEGIVGDDTDGHIDD
                     IARFVAPDTIVCALEDDPADPNYELLRDNYRRLQLARDARGASYRIETLPMPGMVGGR
                     EADRLPASYANFYIANNVVLLPVFNHANDARAADTLQRLIPERCVIPINAESLVWGMG
                     TIHCLSQQQPAV"
     gene            1446..1586
                     /locus_tag="VGB76_06720"
     CDS             1446..1586
                     /locus_tag="VGB76_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098631.1"
                     /translation="MKAETTELYVIGVAMIFFLALCVVAVWAFVRQYRREHPKDEGGR
                     RE"
     gene            complement(1618..2496)
                     /locus_tag="VGB76_06725"
     CDS             complement(1618..2496)
                     /locus_tag="VGB76_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098632.1"
                     /translation="MAATTSTDNNGSRVHPSQTGSHATSPGTPGNQTHDGTASATDPI
                     DQNVLPAEKPGHKELSPKARVLASLTLLIWLNLFAGGIFIDTKPSRCVINPEGVAALG
                     RQSAVNGGRETNPCPVTDTEGRVIERPPLLAAWFLALIFFLPLNLALLCATAGVLGTF
                     GNIANLYPDQLALSSHDHSNPYISGLLRGFFVYLFVISGMLVLDDAPFSNTSPAQYIR
                     LAGFLSLLSFIVNYQPHIFSKLVDMAQNRLPNSQDGAVAVAQTEDDKMAVRGEKVKAT
                     ENRDGTMTLQAEQAKD"
     gene            complement(2727..3209)
                     /gene="def"
                     /locus_tag="VGB76_06730"
     CDS             complement(2727..3209)
                     /gene="def"
                     /locus_tag="VGB76_06730"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0098633.1"
                     /translation="MKITEYPAKVLNELGDSVEKFDEALARLCDDMFETMYAEEGVGL
                     AAPQVGLSLRLFVMDCAGIKLVAANPEIVGTEGEQAGEEGCLSVGKVHSPLKRAARAT
                     LRAQDVRGEWYEREAEGLAARCFQHETDHCDGILFINRLTPLKRNMVTKRFQKLKSWR
                     "
     gene            3279..4127
                     /locus_tag="VGB76_06735"
     CDS             3279..4127
                     /locus_tag="VGB76_06735"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY0098634.1"
                     /translation="MKQYHDLLKSILSHGTTHRDRTGVGTISHFGYQTRFDLRESFPI
                     VTTKKIPFRWIAEELFWFLSGDTNEATLRARGVDIWAEWADEAHTARFNRQAGDLGPV
                     YGYLWRSFGGHYPARDGVDQIARLVAGILENPNSRRLIVTGWDPRVCEEVDLPPCHTL
                     FQFKVEAEKVLHCQLYQRSADAFLGVPFNISSYALLTRMLAHVCDLEAGDLIYTLGDY
                     HIYQNHLEQVHELLSREPLPLPQLEIRDEARRLRGLAGLLDMRYENLNLVGYKAHGKI
                     AAPVAI"
     gene            4182..4661
                     /locus_tag="VGB76_06740"
     CDS             4182..4661
                     /locus_tag="VGB76_06740"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY0098635.1"
                     /translation="MSIIGIVAVDRNGAIGKGGAIPWHYSADMKFFREQTTGHACVMG
                     RRTWASLKRPLRERLNIVLSRSAEVETEAENVIVLPEKRAVLALAPYLSCHLYIIGGE
                     QIYKTFQPEIERWLVTEIPLSIDDADTFMPENYLQNFSPRGSRSLADELQVTVYERT"
     gene            4995..>5255
                     /locus_tag="VGB76_06745"
     CDS             4995..>5255
                     /locus_tag="VGB76_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0098636.1"
                     /translation="MSDHALAQRAASGDLAAFEQLYERHNRRVYSLCLRMTQNVAEAE
                     DLAQEAFIQLFRKIGSFRGESAFTTWLHRLTVNQVLMHFRKRG"
BASE COUNT         1076 a         1578 c         1522 g         1079 t
ORIGIN      
        1 gcttgggaga cgattcaacg cggccacgcg attgccaacg gcgtctatgt cgtggtcgtc
       61 aaccgcgtgg gggcggaggg taagttgaag ttttggggtc agtcgttcgt cgtcgatccg
      121 ttcggtcgca tcgtggcgaa agggtctgcg gacaaagagg aggtgctcat cgtcgaatgc
      181 agcctcgata acatcgagcg cacgcggcag aactggcctt tcctgcgcga ccgccgcatc
      241 gaatcctatc agggcttagg ctaccgcttc ctcgaaacgc cttcgaccaa atcctgacga
      301 aggggaacga gccgtttgcc ttttgagatg aacgcgaagc agcgaataat taagtagatg
      361 aaccagcagc agactcccgc cgcactcggc ttccgcatgc cgcccgaatg gcacaggcac
      421 gccgcgacgt ggctcacgtg gccgaaagac ccgctcacgt ggcctgaccg cgtgccgcaa
      481 gtggaagaga tttacttgca gatcatcgcc gcgctcacgc cgcacgaact cgtcaatttg
      541 ctggttgacg atgaagctac ggaagcgtcc gtgcgcgcgc gttgcaagtt tgaggcgagc
      601 gagcgggtgc gctttcatcg catcccgacg gttgattcgt ggatccgcga ctacggcccg
      661 aacttcctca cgagcgacga cggcaaactc gcctacaacg attggatttt caacgcgtgg
      721 ggcgacaagt atgaagagtt gaagagagac gatgcgatcc cgaagcgtct ggagagcctg
      781 ctcggcgtgc cgcgcttcga gccgggcatc gtgttggaag gtggctcgat tgaagtcaac
      841 ggcgcgggcg tcgtgttgac gaccgagcaa tgcctgctca acccgaaccg caacccgcaa
      901 ctcgaacgcg ccgaggtgga aacttattta aagaactatc tcggcgtcga acaggtgctc
      961 tggctcggcg aaggtatcgt cggcgacgac acggacgggc acatcgacga catcgcgcgc
     1021 ttcgtcgcgc ccgacaccat cgtctgcgcg ctcgaagacg accccgccga tccgaactac
     1081 gaactgctcc gcgacaacta ccgtcggctg caactcgcgc gtgatgcgcg cggcgcgtct
     1141 tatcgcatcg agacgctgcc gatgcccggc atggtcggcg ggcgcgaagc tgatcgccta
     1201 ccggcgtcct acgcgaattt ctacatcgcc aacaacgtcg tgctgctccc cgtcttcaat
     1261 catgcgaacg acgcgcgtgc ggcggacaca ctgcaacgcc tcatacccga acgctgcgtg
     1321 attcccatca acgccgagtc gctcgtctgg ggcatgggca cgattcactg tctatcgcaa
     1381 cagcaacccg cagtttagtc ttaccggatg agaagaggcg cgcgcgccgc aggagcaact
     1441 gaagcatgaa agcggaaacg acggaactgt acgtgatcgg cgtggcgatg atcttcttcc
     1501 tcgcgctctg cgtcgtcgcc gtctgggcgt tcgtgcgtca atatcgccgc gagcatccga
     1561 aagacgaggg cgggcggcgc gaataaacaa caccctgccc accctctcta tcaactacta
     1621 atctttagct tgctcggctt gcagagtcat cgtcccgtct ctattttccg tagccttcac
     1681 cttttcaccc cgcacggcca tcttgtcgtc ttccgtctgc gccacagcca ccgccccgtc
     1741 ctgactgttg gggagtctgt tctgcgccat gtcaaccaac ttactaaaaa tatgcggttg
     1801 atagttgacg atgaagctga gcagcgacag gaatcctgcg agacgtatgt actgtgcggg
     1861 ggaagtgttg gagaacggcg catcgtcgag gacgagcatt ccggaaatca cgaacaggta
     1921 cacgaagaac ccgcgcaaca atcccgaaat gtacgggttg ctgtgatcgt gcgaagagag
     1981 cgcaagttga tcgggataca ggttggcgat gttgccgaac gtgccgagca cgcccgccgt
     2041 ggcgcatagc agagcgagat tcaagggcag aaagaagatg agggcgagaa accatgcggc
     2101 gagcaatggc ggcctctcta tcacccggcc ttccgtgtct gtcacgggac acgggtttgt
     2161 ctctcttccg ccgttaaccg ccgactgtcg ccccagcgca gcaacgccct ccgggttgat
     2221 tacgcagcgc gacggtttag tgtctatgaa aatgccgccc gcgaacaggt tgagccagat
     2281 caggagggtg agcgaggcta acacccgcgc cttaggactc agttccttgt gtcccggctt
     2341 ttcagcaggc aaaacattct ggtcaatggg gtcggtcgca gaagcagttc cgtcatgggt
     2401 ttgattaccg ggcgtgccgg gggatgtggc gtggctgcct gtctgtgagg ggtgaacgcg
     2461 gctaccgttg ttgtcggtgg atgtggttgc tgccatggat ttcttccttg aagagtggtg
     2521 agttacagga gaaatgcctc agttcacatc atgagttgat gggattcggg aactgcggcg
     2581 gaaaaggatg ttcgcgaggt gcggggcgtt tgagcgtctc gccttaggag ttctatcggg
     2641 ctgcgccaga atatgtcaac ttcaaaatca agtcaacaaa cttttgcgcc gtcggcgaag
     2701 ttaaagcgaa gccgtaagcg acgcacttaa cgccacgatt tcaacttctg aaaacgcttt
     2761 gtcaccatgt tgcgtttaag cggggtgagg cgattgatga agaggatgcc gtcgcagtga
     2821 tccgtctcgt gttggaagca gcgtgcggcg agtccttccg cttcgcgttc gtaccattcg
     2881 ccgcgcacgt cctgcgcgcg gagcgtcgcg cgtgcggcgc gcttcaaagg cgagtgaact
     2941 ttcccgaccg agaggcagcc ctcttctccg gcctgttcgc cctccgtgcc gacgatctca
     3001 gggttggcgg ccacgagttt gatcccggcg caatccatca cgaagaggcg cagcgagagg
     3061 ccgacctgcg gcgcggccaa gcccacgccc tcttccgcat acatcgtctc gaacatgtcg
     3121 tcacatagcc gcgccaacgc ctcgtcgaat ttttctacgg agtcgcccag ttcgttcaac
     3181 accttcgccg gatactcggt tatcttcatc accgccatct cttttatttt gtcccgttcg
     3241 cttgtgatat gcttcgcgtt ccgaaaattt tactcgatat gaaacaatat cacgacctgc
     3301 tcaaatccat tctctcccac ggcacgaccc atcgcgaccg cacgggcgtc ggcacgatct
     3361 cccacttcgg ctaccagacg cgcttcgacc tgcgagagag tttccccatc gtcaccacca
     3421 agaagattcc ctttcgctgg atcgcggagg aactcttctg gtttctttcc ggcgacacga
     3481 acgaagcgac tctgcgtgcg cgcggcgtgg acatctgggc ggaatgggct gacgaagcac
     3541 acaccgcgcg cttcaaccgt caggcgggcg atctcggacc cgtctatggt tatctgtggc
     3601 gttccttcgg cgggcattat ccggcgcgcg acggcgtaga ccagatcgcg cggctcgtcg
     3661 caggcatcct tgagaatcca aactcgcgcc gcctcatcgt gacgggctgg gacccgcgcg
     3721 tgtgcgagga ggtggatctc ccgccctgtc atacgctctt ccagttcaaa gtcgaagccg
     3781 aaaaagtttt gcactgccag ttgtatcaac gttcggctga tgcgttcctc ggcgtgccgt
     3841 tcaacatcag ttcctacgcg ctgctgacgc gcatgctcgc gcacgtatgc gacctcgaag
     3901 cgggcgacct catctacacg ctcggcgatt atcacatcta ccagaatcat ctggaacagg
     3961 tacacgaact gctctcacgc gagccgctgc cattgccgca actggagatc agagacgaag
     4021 cgcgacgcct gcgcgggctg gctggactgc tcgacatgcg ctatgaaaac ttgaatctcg
     4081 tcggctataa agcgcacggg aaaatcgccg ctccggtggc gatctgaatt cggggcgggc
     4141 gtcgttcaaa gagcggcacg aattttagtg ttaataattt catgtccatc atcggcatcg
     4201 tagcagttga tcgaaacgga gcaatcggca agggcggcgc gatcccctgg cactattccg
     4261 ccgacatgaa atttttcagg gagcagacga ccggccacgc ctgcgtgatg ggacggcgga
     4321 cgtgggcttc tctgaagagg ccgctcaggg agcgtctgaa tatcgtgttg agcaggtctg
     4381 cggaagtgga gacggaggcg gagaacgtca tcgtcctgcc ggagaagcgc gcggtgctcg
     4441 cgcttgcgcc ttatctttcg tgccacctct acatcatcgg cggcgaacaa atctacaaga
     4501 ctttccagcc cgaaatcgag cgttggctcg tgacagagat tccgctctca attgacgacg
     4561 ccgacacgtt catgcccgaa aattacttgc agaactttag tcccagaggc tcgcgctcac
     4621 tcgcggacga acttcaagtg accgtctacg aaagaacctg acctgcccct ttttttgctc
     4681 gctcaagcgc ggtggagcag acgtaaaaga cagcttccgg gtacgagttt tcggatcgag
     4741 ccgccgccgc caaaaattcc ctgcgcctgc cattttttcg ctgctacacg ctttgcgttc
     4801 aggcatgccc gatatggtcg ggtgcgccgt ttctgctgcc gcgcggcgtc gaattgcgcg
     4861 cccaacaggg ctgttttgcg tggcaccgca cttgctctta taacgacact atgaccaccg
     4921 acgccacaac ctatcccatc tggcaaaacg ccgaaaccgc atctaccact gcgaccgaga
     4981 aagcgcccct tcccatgagc gatcacgccc tcgcgcaacg cgcggccagc ggcgacctgg
     5041 cggcatttga acaactctac gagcggcaca accggagggt ctattccctc tgcctgcgca
     5101 tgacccagaa tgttgccgaa gccgaagacc tggcgcagga agccttcatc caactctttc
     5161 gcaagatcgg ctcatttcgc ggcgagtcgg cttttaccac ctggctccat cgcctgacgg
     5221 tcaatcaggt cttgatgcac tttcgcaagc gcggc
//
LOCUS       DASWNS010000200         9248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_8981, whole genome shotgun sequence.
ACCESSION   DASWNS010000200 DASWNS010000000
VERSION     DASWNS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9248
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8981"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VGB76_06750"
     CDS             <1..967
                     /locus_tag="VGB76_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0098637.1"
                     /translation="PPALSDFNPAACYIAQLQPSGRPSTRARLSRVARLLGATLGTLR
                     WQDLRYEHVVAVRAWLEEQKASPATVNMTLSALRRVAREAFSLGQMESDAYLRIRNVS
                     GIRASVLPAGRALARTEISVLLEVCLADESPAGARDACLVALLASGLRRAECAGLTVA
                     DAFAQAGHLLVRGKGLKERLIPLEAGVRSALDGWLVVRGVRPGALLCPVDKGGRIAAR
                     HLSGQAVTDALARRSREAGLAHVSPHDLRRTFVSQLLERGADIDMVRDLCGHSSIQTT
                     SRYNRRGERARKQTIDLLSLPWPTPRPKRRPKRARKGRRRRRRKS"
     gene            complement(1944..3101)
                     /locus_tag="VGB76_06755"
     CDS             complement(1944..3101)
                     /locus_tag="VGB76_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098638.1"
                     /translation="MNQIRLSDTEVRKKIFLIVSASALAVVVAAASAEIYLRWKQKPA
                     LPEYSLEFYTHNGQKISAQVGSLKLSLAPFTVYKNVPSQQTPTFTINSRGLRADEKAE
                     RDPSPKIIFLGGSAAFGQGSRTDQETIQYILEQSVKPYRVLNAGVVGFLSGQELTYLV
                     TELVDYRPAIVIAYDGWNDLFDTVYSPERTNEELGFSSNFFSIEEQLRLNHQTKVSPF
                     RSLRRMVEAMAKKSLFISHVAYRLHASQLPERVNHKERLEAAVLTYVTNLRKMALFSR
                     ASGARFIVIFQPELGQKTHLTAAEQKMLNEGFQAPDYEEGFPGLYRQFLAEAKQLLTQ
                     EGIEWLDINENPKYLESRDELFIDVVHTNRRGNELVAEIISPRIRALTNSD"
     gene            3327..3749
                     /locus_tag="VGB76_06760"
     CDS             3327..3749
                     /locus_tag="VGB76_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098639.1"
                     /translation="MKAERLGSAGLTILIRCTCYYRLYIDRAEWAFFQIAVCDNCGAL
                     ISYPSFQIRTFCRKGRCMKGNLDNYEDTPERREIITGFDKLQAQAEQLARQFKAKGDS
                     PVWKHAAHRALNAAQSISNQKCLLAQMWTQEDAASKAA"
     gene            3894..4214
                     /locus_tag="VGB76_06765"
     CDS             3894..4214
                     /locus_tag="VGB76_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098640.1"
                     /translation="MEENKTTSGASAATAKDTKTEPTKAEQPIPYAKSGEVLTTWLNR
                     RISEKGNVRVPQGTKSVLVNQLLPDGRLEVEVPDTDGATSQQQYIAFDETMFDLLLPM
                     LAKL"
     gene            4274..4582
                     /locus_tag="VGB76_06770"
     CDS             4274..4582
                     /locus_tag="VGB76_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098641.1"
                     /translation="MRRAAHVDRNQPEIVAALRAVGATVEHLHAVGGGCPDLLVGYKG
                     TNYLLEVKDGKKVASARTLTREQVLWHATWRGQKVIVEDIEQALRAIGAPISTTQTEG
                     "
     gene            4583..4831
                     /locus_tag="VGB76_06775"
     CDS             4583..4831
                     /locus_tag="VGB76_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098642.1"
                     /translation="MMNDESRKHLPIEERGGPSETTVTHVYSGTVICTNPASESDEIR
                     LVIDKRTHNLSIPKEKEDESFVYIYARPKQAERMNDEG"
     gene            4828..5091
                     /locus_tag="VGB76_06780"
     CDS             4828..5091
                     /locus_tag="VGB76_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098643.1"
                     /translation="MNKPTIGRIVRYVAYGTPGGEHPSTERAAIITEVDDPDDEHSPV
                     GLAVLNPTGLFFNQHVPYGDTGGHWHWPELPSHISTACMTSDK"
     gene            5560..5778
                     /locus_tag="VGB76_06785"
     CDS             5560..5778
                     /locus_tag="VGB76_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098644.1"
                     /translation="MNSWRTSVIGAVLIVGAVASLVISALVNKRVPATEDLMLAGGSI
                     ATGVGLISARDNNKSSEDVGANRPSVGY"
     gene            5799..6263
                     /locus_tag="VGB76_06790"
     CDS             5799..6263
                     /locus_tag="VGB76_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098645.1"
                     /translation="MRLKQLFPLLALTLITAFVAACGPNVRAIGRGVEAGTIEFKREF
                     ASMREAGEIDEDTAQKYAVALDEIEATAHDLTLIVNWDAMPKAEKRRTVLIYIGHFAR
                     SAGRLDEAGVLGIKSERTRRRVEAFKRNFKRGLGALRIIEAALPEAEATAKQ"
     gene            6349..6624
                     /locus_tag="VGB76_06795"
     CDS             6349..6624
                     /locus_tag="VGB76_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098646.1"
                     /translation="MLFSIVVPVVFGYGSAQYAQGGITQKVMSIEQKQQATDATVDAN
                     KREGAEQLKDLRKEVVTKEVLDEKWKTVDKMDRKLDELLRLQLKELR"
     gene            6884..7519
                     /locus_tag="VGB76_06800"
     CDS             6884..7519
                     /locus_tag="VGB76_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098647.1"
                     /translation="MKRALRKSAKTPTPQTTTPGSATPDKARAPLALVEHAPAEPAQK
                     SAMEKLGEALSKPPGSSWDDEPDPAVRLRKHVYQQLMNLESMLMLHPDDEDAELLRII
                     KKPIEILRTRLSSTPVRARAAILHSLDRGAQTIPEFVTETRLTRADILKHLDEMIADK
                     VIEKVPDGRTSNKQGPTLYIYIRFGAPTGDNYVAPVRGSSSTARLTSAFDD"
     gene            7610..8182
                     /locus_tag="VGB76_06805"
     CDS             7610..8182
                     /locus_tag="VGB76_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098648.1"
                     /translation="MARQPANLVKQVTFALAQTLTGMVKEAQGEVISDIENTFTVRNN
                     WDKPSNAMGIKALPATKQDLSAALVTRADWLIPHEEGEDKVPEGKALAVPSKNVRRTK
                     RDIIQRSQRPKALRGKRDVVLPLKGGGQGLFQRKGRGTNSKLVFLYALAKRARIRKQP
                     TVVEPTVKVVEKKFDKLFYANLKKALRTAR"
     gene            8179..8637
                     /locus_tag="VGB76_06810"
     CDS             8179..8637
                     /locus_tag="VGB76_06810"
                     /inference="COORDINATES: protein motif:HMM:NF018929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HEY0098649.1"
                     /translation="MNKLGEQLYSISQLASLSGLDRATVTKRLADVPFEEGAKNAKTY
                     ALDAALPALIAGESTEMDAAKLRKTQAEASMRELDLERERGEVVSTSEVADYTLRLFK
                     GVQNRIGLRFPREIAQQLYKAESAAQITEILQRELGRIFNDLRDDHKRFL"
     gene            8669..>9248
                     /locus_tag="VGB76_06815"
     CDS             8669..>9248
                     /locus_tag="VGB76_06815"
                     /inference="COORDINATES: protein motif:HMM:NF017673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HEY0098650.1"
                     /translation="MTVSQWAATYRFLSAERSARPGRWRHEVAPYLVDIMDTVGKPGI
                     REIILVASAQVGKTECCNNILGFFIHADPSPIIFVAETQDKAEAWSKESLAPMIRDTP
                     VLANLVSDARTRDSGNTIGGKSFPGGHLAIAHATSPATLSSRPRRVVLLDERDAFKST
                     AEGDPAKLAEKRATTFPDAVIVKVSTPRDRLEL"
BASE COUNT         2010 a         2751 c         2731 g         1756 t
ORIGIN      
        1 ccctcccgcg ctctccgatt tcaacccggc ggcctgttat atcgctcaac tccagccctc
       61 cggacgaccc tcgacccgtg cccggttgag ccgcgtggcg cgccttctgg gggccacgct
      121 cggcacgctc cgctggcagg atttgcgcta cgagcacgtc gtcgctgtgc gcgcctggct
      181 ggaggagcag aaagcctctc cggcgaccgt caacatgacg ctctcggcct tgcgccgcgt
      241 cgcccgcgaa gccttcagtc tcgggcagat ggagtcggac gcttacctgc gcatccggaa
      301 cgtctcaggc atccgggcga gcgtccttcc cgcggggcgg gcgctcgccc ggacggagat
      361 ctccgtcctc ctcgaagtct gtctcgccga cgaatcgccg gcgggcgcgc gtgacgcctg
      421 cctcgtcgct ctcctcgcct cgggactgcg ccgggccgag tgcgccgggc tgaccgtcgc
      481 cgatgccttc gcgcaggccg gtcacctact ggtgcgcggc aaggggctca aggagcggct
      541 tatcccgctc gaagcgggcg ttcggtccgc gcttgacggc tggctcgtgg tgcgcggcgt
      601 gcgcccgggc gcgctgctgt gcccggtgga caagggcgga cgcatcgctg cgcgccacct
      661 ctccgggcag gccgtcacgg acgcgctcgc gcgccgatcg cgcgaggctg gccttgcgca
      721 cgtctcgccg cacgacctcc ggcgcacctt cgtctcgcag ctcctcgaac gcggtgccga
      781 catcgacatg gtcagagatc tctgcgggca ctcaagcatc cagacgacgt cgcgttacaa
      841 ccggcggggc gagcgcgccc gcaaacagac catcgacctg ctctcgctcc cgtggccgac
      901 gccgcgaccg aaacgtcgcc cgaagcgagc gcgcaagggt cgccgccggc ggcgacgtaa
      961 atcgtgagtc ttaagtcgaa ctttgacgga attggacgct ggaggggtgc aatggaaatc
     1021 accgagagac agaaacgggc tggccggaag gtcatcaagg tcgcgcaact gatgcgactc
     1081 gtcgtgcgcg aggctgtgcg cgttcgcgcg ccatccatat gcgtgctgcc gaacgcgggg
     1141 aagaatagcg gaagggaact gggagaggcg agcctgaacc aagtcgcctc tcccttccgg
     1201 cttcctattg cgggtcgggg cacgggccag gaccgtcacc ccctggagcg taaagcgcac
     1261 aacactccgg gctaacacag tgacacccat aatcgaaggg gttgcagtcc cacaattcag
     1321 ggcacggaac atgtgacggg caataggagg cttgtgtagt tgtgagtgaa aacatgaggc
     1381 acacaactag actaagaagc acacagaaac gtttgacgag tcggaatacg tttggtttga
     1441 ccatgaggtg actccctttt ggctgaggtt aatgcgaggc caacgagatg ggaactcgct
     1501 ggcaggaaag tactcgtgag acacggggaa gatatgccct tgtgagatga agtgtcaata
     1561 gggagggttc tagcaggcgg tgtgataaac cctggtgcgg tcgtttgatg tgggcatctc
     1621 gtcgaggccg ctcacgacta gtcgtcgctc actgggactt aacaccagtg aagcgcatcg
     1681 tgccggggag tctgcttggt cagtgagcct taaatacgaa ggcgccgtgt ccttgccagg
     1741 acggctcctc cattgtgggc atcgaagtga tgccgagtcc ggtggctctg aattctatgc
     1801 gaaacgttca gtatgtcaac acaaaaggag ggaacttggg atggttgacg tgggactgcg
     1861 caagtataga aagaggtgaa tcgcctcttc attctcgccg ccttcaaacc ctgcaccggc
     1921 gagcctagaa aggatttccc acgtcaatcg ctattggtca atgctcgtat ccgtgggctg
     1981 atgatctcgg cgactaattc attaccacga cggttagtgt gcacgacatc gataaagagt
     2041 tcatctctgc tctccagata tttcgggttt tcattgatgt cgagccattc tattccttcc
     2101 tgtgtgagta gttgtttagc ttcggccaga aactgtcgat ataagccggg gaatccctcc
     2161 tcatagtcgg gagcttgaaa cccctcattc agcatcttct gttccgccgc cgtcagatgg
     2221 gttttttggc cgagttccgg ctggaagatc acgatgaacc gcgcgccgct ggcacggctg
     2281 aataacgcca tctttcggag gttcgtaacg taggttaaaa cagctgcctc taatcgctct
     2341 ttgtgattga ctctttctgg cagttgcgaa gcatgcagcc tatacgcgac atgactgatg
     2401 aacaggcttt tctttgccat agcctctacc atgcggcgca ggctccggaa tggactgacc
     2461 ttcgtttgat ggttcagccg gagttgctct tcgatgctaa agaagttgct gctgaagccc
     2521 aattcttcgt tggttctttc aggcgagtag acagtatcga acagatcatt ccagccatcg
     2581 taggcgataa caatagccgg acgataatca acaagttctg tgaccaggta agtcaattcc
     2641 tggccggaga ggaacccgac gacccccgca ttcagaactc gataaggctt gacggactgt
     2701 tccaggatgt actggatcgt ctcttggtca gtccgactgc cttggccgaa ggcggcagaa
     2761 ccgcctaaaa agataatctt cggcgaagga tctcgttcag ccttctcatc tgcgcgtaaa
     2821 ccgcgagagt tgatggtaaa tgtcggcgtc tgctgtgatg ggacattttt atagacagta
     2881 aacggtgcga gcgagagctt caggctcccc acttgagcag aaatcttctg gccgttatgc
     2941 gtataaaatt cgaggctata ttcggggagc gcgggtttct gtttccatcg gaggtagatc
     3001 tccgcgcttg cggctgcgac gaccacagcc aacgctgatg cgctgacgat taagaatatc
     3061 ttttttctca cttcggtatc gctcagcctg atctgattca ccactcgaag gtggcttcaa
     3121 ttgagctgtc gaataaagtg gccggcttaa gtctcggcga ggggtgtgag ctagagatgg
     3181 ccgtgataaa tgcccgtgcg gaacgtgagt gaaagatgga ggcagcgtgt ccttaactat
     3241 gaccgccgcc tctgcttgtg ggcatccgac gcgaattcta ttccttttga ataagatgtc
     3301 aagaaaaaga tagacagctc ccaactatga aagctgaacg cttaggcagc gccggactca
     3361 ctatcctcat ccgctgcacc tgctattacc gcctctatat cgaccgcgcc gagtgggcgt
     3421 tcttccagat cgccgtctgt gacaattgcg gcgcgctcat cagttatcct tcatttcaga
     3481 ttagaacttt ctgccggaag gggcgatgta tgaaaggcaa tctcgacaat tatgaagaca
     3541 ctcccgaacg ccgggagatc atcaccgggt tcgacaagct acaagctcaa gccgagcagc
     3601 tcgccaggca gttcaaagcg aaaggcgatt cccccgtctg gaagcatgcc gcgcaccgtg
     3661 ccttgaacgc cgcgcagtcc atctccaacc agaagtgctt actggcgcag atgtggacac
     3721 aagaagacgc cgcctcgaag gcggcctaga tacacgaacg cccgcagtag caagcgccga
     3781 cctgcgggca tccatgaaaa gagtttctcg agaactcgtg ctccgatcct attcactcac
     3841 agcatagccc gaaggctatt gctcgtcaat tactttgaca taggagcaca aacatggaag
     3901 agaataaaac caccagcggc gcgagcgctg ccaccgccaa agacaccaag accgaaccca
     3961 ccaaggccga gcaacccatc ccttacgcca aatccggcga agtcttgacc acgtggctca
     4021 atcgccgcat cagcgaaaaa ggcaacgtca gagtcccaca gggcaccaag agcgtgctcg
     4081 tcaaccagtt gctacccgac gggcggctcg aagttgaagt gcccgacact gatggtgcca
     4141 cctcacaaca acaatacatc gcctttgacg agacgatgtt cgatttgttg ttgccgatgc
     4201 tggcgaagct ctaacgccct ccccagccgc gtgcgctgct ttgtacggta gcgcacgcgg
     4261 ccttctcagc tctatgcgtc gagcagccca cgtagaccgc aaccagccgg agatagtcgc
     4321 cgccctgcgt gccgtgggcg cgaccgttga gcacctgcat gctgtgggcg gcggttgccc
     4381 cgatctgctt gtgggctaca agggcacaaa ctacctgctc gaagtgaaag acgggaagaa
     4441 ggtggccagc gcgcgcacgc tgacccgtga gcaggtcttg tggcacgcca cctggcgcgg
     4501 ccagaaggtc atcgtcgagg acatcgaaca ggcgttgcgc gctatcggcg caccaatctc
     4561 aaccacacag acggaaggat gaatgatgaa tgatgaaagc cgtaagcact tgccaattga
     4621 agaacgtggt gggccttcag aaacaaccgt cacgcatgtc tacagtggca ctgttatctg
     4681 cactaacccc gcaagcgagt cggatgagat tcgactcgtg attgataagc gaacacacaa
     4741 tctctctatt cctaaagaga aagaagatga gagctttgtg tatatctacg ctagaccaaa
     4801 gcaggctgaa aggatgaatg acgaaggatg aacaaaccta caatcggtcg catcgtgcgt
     4861 tacgtggctt atggcacgcc gggcggagag catccgtcca ccgagcgcgc agccatcatc
     4921 accgaggtgg acgatccgga tgacgagcat agtcccgtcg gcttggccgt gctcaacccg
     4981 accggcctct tcttcaacca gcatgtgccc tatggcgaca ctggtggaca ctggcactgg
     5041 ccagagttgc cgtcccatat cagcactgcg tgtatgacga gtgacaagtg ataggtgaca
     5101 agtgccgggt gacaggtgag aggtgagagg ggcgaggtgc catcccgcgt caccctgccg
     5161 ccacttcctg tcactcgacg cgacctcgcg cgctcctcgc agcctcaagc gcgctctcgg
     5221 cgcgcgggtc cttccccgac ctctcaactc tgagggtaac gcacaacgcg caaggggtcg
     5281 ctagctgtag aaatttattt catttcatca gcagcaggcg ttttgatgcg ctgtttatgc
     5341 gggtttgatg ggctggtgct gcaggtggct gcgtcgaggc ggggcggctg ccatgtttct
     5401 accttgtggt gcgccgcagg tgagccgaaa ggcgaaccgg aggctctgag cgggtgcata
     5461 ggcttggcga gggggtcgcc aaaatcataa aaactgagtt ctggctatgc ttggggaatg
     5521 gcagcccact gcagggctac agacccaagg gagatgcaca tgaatagctg gagaacttct
     5581 gtaatcggcg cggtgctcat cgtcggcgcg gtggcgtccc tcgtgatctc ggcgctcgtc
     5641 aacaaacgcg tgcccgcgac ggaagattta atgctcgccg gcggctccat cgccaccggc
     5701 gtcggcctca tctccgcacg cgacaacaac aaatcgtcgg aggacgtggg cgcgaatcgc
     5761 ccgtccgtcg gctattaacc ccggagagaa aattctttat gagactaaaa caactgtttc
     5821 ccctcctcgc cctgaccctc atcactgcct tcgtggctgc ctgtggccct aacgtgcgcg
     5881 ccatcggacg gggcgtcgag gcgggcacca tcgagttcaa gcgggagttc gcttccatgc
     5941 gggaggctgg cgagatcgac gaagacacgg ctcagaaata cgccgtcgcc ttagatgaga
     6001 tcgaagccac cgctcacgac ctcactctca tcgtcaactg ggatgcgatg ccgaaggccg
     6061 aaaagcggcg caccgtgttg atctacatcg gccactttgc gcgcagcgcg gggcggctcg
     6121 atgaagcggg cgtgctcgga atcaagagcg agcgcacacg ccggcgggtg gaggcgttca
     6181 agcgaaactt caaacgggga ctcggcgcgc tgcgcatcat cgaggcggcg ctccctgaag
     6241 ccgaagcgac agctaagcag taagtacaaa caatttgccg tcatgtcccc caagatcact
     6301 tcgcaaccgg cagcgtcagg atggatagag cacccgttat ttctcggggt gctcttttcc
     6361 atcgtcgtgc cggtcgtttt cggctacggc tcggcgcagt acgctcaggg cggcatcaca
     6421 cagaaggtta tgtccatcga gcaaaagcag caggccacgg acgcgaccgt ggacgccaac
     6481 aagcgagagg gcgctgaaca gctcaaagac ctccgtaaag aggtcgtcac caaagaggtg
     6541 ctcgacgaga agtggaagac ggtggacaag atggaccgca agctggacga actgctccgc
     6601 ctgcagttaa aggaactccg gtgacagacc gactacttca cacggaaatt ctgagagacg
     6661 ccggcggcgt cgctcacttg atgggcgtgt atgtgaccgc cgatggtgcg atctacatcc
     6721 gcgacttagg gccgacgcta tgaaaacttc tcccccggaa acaaacaacg attcccgtga
     6781 cggggtaacg ctcgtcgcct tctcgtttct caccgccgcg gcgtgggtcg ccggctgcgt
     6841 cgtgatgttc gggctggctt atctttcgaa ccctgggcac taaatgaaaa gagctttacg
     6901 caaatccgcc aaaactccca ccccgcagac caccaccccc gggagcgcga cgcccgataa
     6961 ggcgcgcgct cctctcgccc tggttgagca tgcaccggca gagccggcac agaagtcggc
     7021 gatggagaaa ctcggcgagg cgctctcaaa gcctccgggc tcgtcgtggg acgatgaacc
     7081 cgatccggct gtccggctgc gcaaacacgt ctaccagcaa ttgatgaacc tggaatcaat
     7141 gctgatgctg catccggacg atgaagacgc agaactgttg cgcatcatca aaaagccgat
     7201 agagatcctg cgcacccgac tgagttcgac gcccgtgcgc gcccgtgccg ctatcctgca
     7261 cagcctcgat cgtggagcgc agaccattcc tgagtttgtc accgaaacgc ggctcacgcg
     7321 cgctgacatc ctcaagcatc tcgacgagat gatcgcggat aaggtcatcg agaaggtgcc
     7381 ggacggacgc accagtaaca agcaggggcc gaccctctac atctacattc gtttcggtgc
     7441 gccgacgggt gacaactacg tcgcccccgt ccgcggcagc agcagcacgg ccagattaac
     7501 cagcgccttc gacgactgag cggcccggat ctttccttct ctccccgcgc caaaatcata
     7561 aaagccgaat cctgactatt cttaagaccg ggaaggagtc tctttcggcg tggcacgtca
     7621 accagcaaat ctcgtcaaac aagtcacttt cgcgctcgcg cagacgctca cgggcatggt
     7681 caaagaggcg cagggcgaag tgatctccga catcgagaac acttttacgg tgcgtaacaa
     7741 ctgggacaag ccttcgaacg cgatgggaat taaggccctg ccagcgacga aacaggatct
     7801 ctcagccgcg ctcgtcacgc gcgccgactg gctcattccg cacgaggagg gcgaagacaa
     7861 agtcccggag gggaaagcgc tggccgttcc gagtaagaac gtgcggcgca cgaagcgcga
     7921 catcatccag cggagccaga ggccgaaggc gctacgcggc aagcgcgacg tggtgctgcc
     7981 cctgaagggc ggcggccagg ggctgtttca acgaaaggga cgcggcacta acagcaagct
     8041 cgtcttcctc tacgcgctgg cgaagcgtgc tcgcatccgc aagcagccca cagtcgttga
     8101 gccgacggtg aaagtcgtcg agaaaaagtt cgacaaactc ttttacgcga acctgaagaa
     8161 agcgctgagg acggcccgat gaacaaactt ggggaacaac tctattccat ctcgcaactc
     8221 gcgagcctca gcggcctcga ccgtgcgacc gtcacgaagc ggctggccga cgtccctttc
     8281 gaggaagggg caaagaacgc caagacttac gcgcttgatg cggcgctgcc cgcgctcatc
     8341 gccggtgaat cgacagagat ggacgcggcg aaactccgga agacgcaggc cgaagccagt
     8401 atgcgtgaac tcgacctgga gcgagagcgc ggcgaagttg tctcgacgtc cgaagtcgct
     8461 gattacacgc tgcgcctctt caagggcgtg cagaaccgca tcggcctcag gttcccgcgc
     8521 gagatcgcgc agcagcttta caaggccgag tcggcggcgc agatcacgga aatcttacag
     8581 cgcgagctgg ggcgcatctt taatgacctt agagacgatc acaagcgttt tctctgaggc
     8641 cattgcctcg gcgatcccgg aatcatcttt gaccgtctcg cagtgggcgg cgacctacag
     8701 gtttctctcg gctgagagga gcgcacgacc gggccgctgg cgtcacgagg tagcgccgta
     8761 cctggtggac atcatggata cggtcggcaa gcccggcatc cgggagatca tcctggtcgc
     8821 ctcggcgcag gtgggaaaaa ccgagtgctg caacaacata ctcggtttct tcatccacgc
     8881 cgatccgtcg ccgatcatct tcgtcgccga gacacaggat aaagcggaag cctggtcgaa
     8941 ggagtcgctc gcgccgatga tccgcgacac ccctgtgctc gcaaacttgg tttcggacgc
     9001 gcgcacgcgc gactcgggca atacaatcgg gggcaagtct ttccccggcg gtcatctggc
     9061 catcgcgcac gcgaccagtc cggcgacgct ctcctctcga ccgcgtcgcg tcgttctcct
     9121 cgacgagcgc gacgccttca aatccaccgc cgaaggtgac ccggcgaagc tcgccgagaa
     9181 gcgtgcgacg accttccccg acgctgtcat tgtgaaagtc tcaacgccgc gcgaccggtt
     9241 ggaactgg
//
LOCUS       DASWNS010000201         4566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_216349, whole genome shotgun sequence.
ACCESSION   DASWNS010000201 DASWNS010000000
VERSION     DASWNS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216349"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..473)
                     /gene="rplU"
                     /locus_tag="VGB76_06820"
     CDS             complement(165..473)
                     /gene="rplU"
                     /locus_tag="VGB76_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY0098651.1"
                     /translation="MSYAIIRSGGKQFRVSAGETVRVPSLGEQAGASVEFDVLVVGGD
                     NETKVGSPIVDGARVAGTVVEHGRGDKIIVFKKKRRKQYKRTQGHRQNYTAVKIDSIG
                     "
     gene            complement(577..1971)
                     /gene="accC"
                     /locus_tag="VGB76_06825"
     CDS             complement(577..1971)
                     /gene="accC"
                     /locus_tag="VGB76_06825"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY0098652.1"
                     /translation="MAIAPRKFRKILIANRGEIACRIIWACKEMGIQTVTVHSEADRD
                     SLHVRFADQSICIGAAPSAQSYLNIPAVISAAEIMNVDAIHPGYGFLAESSYFAEICE
                     ACNIKFIGPRSSVIKLMGDKVEARRAMAAAGVPILPGSPDPLETEAEALRLAREIGFP
                     VIIKAAAGGGGRGMRIVRTEGELGQALETASNEALASFKNGDVYIERYVERPRHVEIQ
                     VMADEFGNCVHLGERDCSVQRRHQKLIEEAPSPALSRELRERMGTVAVEACKRVGYSN
                     AGTVEFLLDEDGSFYFMEMNTRVQVEHPVTEMITLADIVRNQIRVAEGEELAFRQEDV
                     IFAGHSIECRINAEHPETFVPSPGLITACNLPGGPGVRVDTAIYPGYFVPPYYDSLIA
                     KLIVHARTRELAIARMRRALESMVIEGIKTTIPLHLKIMDDAQFQSGDFSTKFLEEFL
                     ARNGDRSGVKIANA"
     gene            complement(2066..2845)
                     /gene="accB"
                     /locus_tag="VGB76_06830"
     CDS             complement(2066..2845)
                     /gene="accB"
                     /locus_tag="VGB76_06830"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY0098653.1"
                     /translation="MSDEKRLETPGSQPTGDSSGGGSSSSSAARQSPADTRRGGAAGE
                     VNNSNVGGGGRHGSRRRHRSGGERENQSAPRRPETSINMDELRELADLIAAQGFTEFE
                     LEKEGFRLRLSRNIYSQSPPQTTGSGTGSVIPFPAPPTAATSAQTQAPNSAPTSAHTS
                     STAAATAPVLAAAEAPSAVEDLYKITSPIVGTFYRAPSPTSDVFVNQGSRVEAETVVC
                     IIEAMKLMNEIQAEVSGTIEKIYVENGQPVEYGQALFGVKR"
     gene            complement(3052..3792)
                     /gene="rplQ"
                     /locus_tag="VGB76_06835"
     CDS             complement(3052..3792)
                     /gene="rplQ"
                     /locus_tag="VGB76_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY0098654.1"
                     /translation="MRHLKAHRKLGRTTEHRLSLLRNLATSLINARNERVVTTLPKAK
                     ELRPFIERVITLSRKAQTLEGDDAAVQAVHLRRQAATFFHTGNRQMKSETGRRGVVRP
                     VRTAGVAAVKRLFDELGERYKDRPGGYTRIIKLGRRDGDNAELAIIELVDSPRELEAA
                     KQTEKRAKSVTSDKKRSARASKSGEAEGGRRGGRKADKTYEREETTAKRGRGKTGGED
                     VKDADKAGSGGGRSKKTESTAAEQDTDK"
     gene            complement(3886..>4566)
                     /gene="rpoA"
                     /locus_tag="VGB76_06840"
     CDS             complement(3886..>4566)
                     /gene="rpoA"
                     /locus_tag="VGB76_06840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY0098655.1"
                     /translation="ADVEVLDPNVYIATVSEGGSLNIEMRLKRGRGYVPADRNFDEDL
                     SLGYIPVDSVHTPVKKVNYTVEAARLGQNTEFDKLTIDVWTDGSVKPDDAVGLAAKLI
                     KDHMAIFINFEEDTDEFAYTGIERPPLPRNDQLDRSVDELELSVRSYNCLKNANIKSI
                     RDLVQRSEREMLATKNFGKKSLNGIKDILHGMGLDFGMEFDEQGNPIPGTGGRDSDIS
                     DFADEDEE"
BASE COUNT          793 a         1444 c         1319 g         1010 t
ORIGIN      
        1 gccccaaccg ctgcgactgc gaatcgcgcc cgttacgcga actgcccact ccctttttat
       61 gtgccataac tcaacccctc aaagtgatga gtaatgagtg atgagtaatg agtgagagtt
      121 aaatgctttt actcatcact cattactcat cactcatcac ttatttatcc aatcgaatcg
      181 atcttcaccg ccgtgtaatt ttgccggtgg ccttgtgtgc gtttgtactg cttgcgccgt
      241 ttcttcttga agacgatgat cttgtcgccg cgcccgtgtt cgacgacagt gcctgccacg
      301 cgcgcgccgt cgacgatggg cgaaccgacc ttcgtttcgt tgtcgccgcc cacgacgagc
      361 acgtcaaact ctacgctcgc gcccgcctgt tcgccgagcg aaggcacgcg caccgtctcg
      421 cccgccgata cgcggaattg cttgccgccc gatctgataa ttgcgtaaga catctctagt
      481 tactctcctc agacgcgaaa atcccttgcg ggaaagtcgt tcattataaa ggctgctttc
      541 gggctggtca acgcgcgaaa gccgttcatt tcaaactcag gcgttcgcga ttttcacgcc
      601 gctccggtcg ccgtttcgcg ccagaaactc ttccagaaac ttggtggaaa agtcgcccga
      661 ctgaaactgc gcgtcgtcca tgatcttcaa gtgcagaggg atcgtcgtct tgattccttc
      721 gatgaccatt gattcgagcg cgcggcgcat gcgcgcaatc gccagttcgc gcgtgcgcgc
      781 gtggacgatg agtttcgcga tcagcgagtc gtagtagggc ggcacgaaat agccggggta
      841 gatggccgtg tccacgcgca cgccggggcc tcccggcaag ttgcaggccg tgatcaatcc
      901 gggtgaaggc acgaacgtct ccgggtgctc cgcgttgatg cgacattcga tggagtgtcc
      961 ggcgaagatc acgtcctcct gtctgaaggc cagttcctcg ccctccgcga cgcgaatctg
     1021 attgcgcacg atgtcggcca gcgtgatcat ctccgtcacc ggatgctcga cctgcacgcg
     1081 cgtgttcatc tccatgaaat agaaactccc gtcctcgtcg agtagaaact cgaccgtgcc
     1141 cgcgttcgag tagcccacgc gcttgcacgc ttcgaccgcg accgttccca tgcgctcgcg
     1201 caactcgcgc gagagcgcag gcgaaggagc ctcttcgatc aacttctgat ggcgacgctg
     1261 aaccgaacaa tcgcgctcac ccagatgcac gcagttgccg aactcgtcgg ccatcacctg
     1321 aatctcgacg tgacgcggac gctccacgta gcgttcgata tacacgtcgc cgttcttgaa
     1381 cgaagcgagc gcttcattcg aagccgtctc caacgcttgt ccgagttcgc cttcggtacg
     1441 gacgatgcgc atgccgcgcc cgccgccgcc cgccgccgct ttgatgatga ccggaaaccc
     1501 gatctcgcgc gccagtctca gagcttccgc ttccgtctcc aaagggtcgg ggctgccggg
     1561 caggatcggc acgcccgccg cggccatcgc gcgccgcgcc tccaccttgt cgcccatcaa
     1621 cttgatcaca gacgagcgcg gcccgatgaa cttgatgttg cacgcctcgc aaatctccgc
     1681 gaagtaggaa gactcggcga ggaatccgta acccggatga atcgcatcca cgttcatgat
     1741 ctccgccgcg ctgataactg ccgggatgtt caaataactc tgcgcggaag gcgctgcgcc
     1801 gatgcagatc gactggtctg cgaagcgcac gtggagcgaa tcgcgatccg cctccgagtg
     1861 cacggtgacg gtctggatgc ccatctcctt gcacgcccag ataatccggc acgcgatctc
     1921 gccgcgattc gcgatcagga tcttccgaaa tttgcgcgga gcaattgcca taatcaagtg
     1981 acgggtgaca agtgacgggt gacgagttag agacaagttt tttgatttaa cttgtcacgc
     2041 gtcacctctc actcgccacg tttctctacc tcttgacccc gaagagagcc tgcccgtatt
     2101 cgacgggctg gccgttctcg acgtaaattt tctcaatcgt gccgctgact tcggcctgta
     2161 tctcgttcat caatttcatc gcttcgatga tacacaccac cgtctccgct tcgacgcggc
     2221 tgccttgatt gacgaacacg tcggaggtcg gcgaaggcgc gcgatagaac gtgccgacga
     2281 tgggcgaagt gatcttgtat aaatcttcaa ccgcgctggg agcttcggca gcggcgagca
     2341 caggcgccgt agcggcggca gtcgaagagg tgtgcgcgct tgttggcgca gagtttgggg
     2401 cttgcgtctg agcggaggtg gcggcagtgg gaggcgcggg aaacgggatg accgatcctg
     2461 ttccgctgcc ggtcgtctgc ggcggactct gcgaataaat gttgcggctc aggcgcaggc
     2521 gaaagccttc cttctccaac tcgaactcgg tgaaaccctg cgcggcgatc aaatcggcca
     2581 actcgcgcaa ctcgtccatg ttgatcgaag tctcaggccg acgcggcgcg ctctggttct
     2641 cgcgctcccc gccgctacga tgacgacgac gcgagccatg acgaccgcca ccgcctacgt
     2701 tgctgttgtt gacttcaccc gccgcgccgc cgcgccgcgt gtcagcgggc gactggcgcg
     2761 ccgcactgct gctgctgcta ccgccgccgc tgctgtcgcc cgtcggctgg ctaccgggtg
     2821 tttcgagtct cttctcgtcg ctcatgtttg gtgacacctc cgcgcgtcgt gagtcgctag
     2881 cgctctcgct gcttgcgttg gtagagcaaa atcaaagccc ctgcccgctc cggctcaagt
     2941 gtcgtcgcgc gagcggggga ttggggctgc tggcttccgt gcgagacatt tgagacgagg
     3001 ggcgcgcttg ctcgtctgac acgagaaacg cgccccgctc gatgctcggc tctatttatc
     3061 cgtatcttgc tcggcagccg tcgattcggt cttcttcgaa cgaccaccgc cgctgcccgc
     3121 tttatccgcg tccttcacgt cttcgccgcc cgtcttacca cgaccgcgtt tggcggtcgt
     3181 ctcttcccgc tcgtaagtct tgtcggcttt acgcccgccg cgtcgcccgc cttcggcctc
     3241 gccggacttc gacgcacgcg ccgatctctt cttatccgaa gtgaccgact tggcgcgctt
     3301 ctccgtctgc ttcgccgctt ctaattcgcg cgggctatcg acgagttcga tgattgcgag
     3361 ttcggcgttg tcgccgtcgc gccgtcccaa tttgatgatg cgcgtgtagc cgcccggacg
     3421 atccttgtag cgctcgccga gttcgtcgaa gagacgcttg acggcagcca cgcccgccgt
     3481 gcggacaggg cgcacgacgc cgcgacgacc cgtttcgctc ttcatctgcc ggttgcccgt
     3541 gtggaagaac gtcgccgcct gacggcgcag gtgtacggct tgcaccgccg catcgtcgcc
     3601 ttcgagcgtt tgcgccttgc gcgagagcgt aatcacccgc tcgatgaacg ggcgcagttc
     3661 tttggccttc ggcagcgtgg tgacgacgcg ctcgttgcgc gcgttgatga gtgaagtcgc
     3721 gaggttgcgc aacagcgaga gacggtgttc ggtagtccgc cccaatttgc gatgtgcttt
     3781 caggtgtcgc atagcttttt tataagtgac aggtgacgcg tgacgcgtga caggaagaac
     3841 aagtctttaa cttgtcactt gtcacctgtc actcgtcact gttggttact cttcgtcttc
     3901 gtccgcgaag tcgctgatgt cggagtcgcg cccgccggtg cccggaatcg ggttgccctg
     3961 ttcgtcaaac tccatgccga agtccaagcc catcccgtgc aggatgtctt tgatcccgtt
     4021 cagcgatttc ttgccgaaat tcttcgtcgc cagcatctcg cgttcgctgc gttgcacgag
     4081 gtcgcggatg ctcttgatgt tggcgttctt cagacaattg taggaacgaa cggacagttc
     4141 gagttcgtca accgaacgat cgagttgatc gttgcgcggc agcggcggac gctcgatgcc
     4201 cgtgtaggca aactcgtccg tgtcctcttc gaagttgatg aagatcgcca tgtggtcttt
     4261 gatcaacttg gccgcaagtc ccacggcatc gtccggcttg acagagccgt ccgtccacac
     4321 gtcaatcgtc aacttgtcga actccgtgtt ctggccgaga cgcgccgcct caaccgtgta
     4381 gttgaccttc ttaacgggcg tgtgcacgga atccaccggg atgtaaccga gcgagaggtc
     4441 ttcgtcgaag ttgcggtcgg cgggaacgta gccgcgcccg cgcttgaggc gcatctcgat
     4501 gttgagcgaa ccgccttcgg agacggtggc gatatagacg ttcgggtcga gcacctccac
     4561 gtcggc
//
LOCUS       DASWNS010000202         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_267666, whole genome shotgun sequence.
ACCESSION   DASWNS010000202 DASWNS010000000
VERSION     DASWNS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267666"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1193)
                     /gene="prfB"
                     /locus_tag="VGB76_06845"
     CDS             complement(join(77..1120,1122..1193))
                     /gene="prfB"
                     /locus_tag="VGB76_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006089.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY0098656.1"
                     /translation="MIETSTVQELRASYEDLRARVEQLGGFFDVASKQEELKSIEGLA
                     SAQDFWNDQEEAQRLLQQRSRLERVINRQQGFESAIGDAGVLFEFAEAGEDAESMREL
                     RALVERLEREIGEAETEMLLAGENDARNAICTVHPGAGGTESQDWAEMLLRMYLKWAE
                     RRGFKTEILDYQEGEEAGIKSATFRVEGEYAYGLLAAEAGVHRLVRISPFDQAARRHT
                     SFASLFVYPEIDENIEVDIQDKDLRVDTYRATGAGGQHINTTDSAVRITHIPTNIVVQ
                     CQNQRSQHQNRAVAMQVLRSRLYELELERRKAETAVLEANKADISFGSQIRNYVLAPY
                     RLVKDARTKLERGDVDAVLAGDLDDFIKAYLLSRRAA"
     gene            complement(1246..>2792)
                     /gene="lnt"
                     /locus_tag="VGB76_06850"
     CDS             complement(1246..>2792)
                     /gene="lnt"
                     /locus_tag="VGB76_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY0098657.1"
                     /translation="LSFPDFTLWPLAWVGLVPVLVAVARVPRTRHAFVLGWTTGALFF
                     YGSCWWLTHSMIHYGGLPRPVAYALLVPVVLIVGLFPATFALALAHLHKRRGIYMLLA
                     APCLWAATEWARLGATGQLWNAVGYSQAFHPALIQPATWGGVYAVGFLLVAVNASVAL
                     VLVHRNARAFNLATLMLIAVGLVFALTESIDFTDHNFDSSEPAAYTVVIAVQPNVSVN
                     FGRSTEENATLVNRHLTMSADALRAWEQEQEAEAKREIERSASSGTRAYIYDPPRVVV
                     WPESPMNFAYARDAEFQTLVGGFARAARASVIFNSLEPAPAGGAYNSAILINEAGRLV
                     AQYDKIRLLPFGEYVPLPRWFPPAWLVSGIVGDFTPGANYTLLPLGETRAGVFICFES
                     AFPSIARTFTGAGADVLINISNDGYLGQTPVLRQHLANAVFRAVENRRNVLRVTNTGI
                     SAHITPRGEVLDATGSFEPAVRTWRVSRATGGKTFYTRYGDLFVALCATVSILLIAAT
                     VKRTVK"
BASE COUNT          548 a          876 c          856 g          512 t
ORIGIN      
        1 cataactttt aagtctcaag tctcaagtcc cgagtttgca actcctgtct ctgctgccgg
       61 acgcagggct ttttccttat gccgcgcgac gcgacagtag ataagccttg atgaaatcgt
      121 ccaggtcgcc tgcgagcacg gcatccacgt cgccgcgctc caacttcgtg cgcgcgtctt
      181 tgacgagacg ataaggcgcg aggacgtagt ttctgatctg cgagccgaaa gagatgtcgg
      241 ccttgttcgc ttcgagcaca gcggtttcgg ccttgcgccg ttcgagttcc aactcataca
      301 ggcgcgagcg caacacctgc atggcgaccg cgcgattctg atgttgcgag cgttggttct
      361 ggcattggac gacgatgttg gtggggatgt gcgtgatgcg caccgccgag tcggtcgtgt
      421 tgatgtgctg tccgcccgcg cccgtcgcgc ggtaggtgtc cacgcgcaag tccttgtcct
      481 gaatgtccac ctcgatgttt tcgtcaatct ccgggtagac gaagagggaa gcgaacgacg
      541 tatgacgccg cgccgcctga tcgaacggcg agatacgcac gaggcgatgc acgcccgctt
      601 ccgccgcgag cagcccgtag gcgtactcgc cttccacgcg aaacgtcgcg ctcttgatcc
      661 cggcctcctc gccctcttga taatcgagta tctcggtttt gaagccgcgc cgctccgccc
      721 atttcagata catgcgcagg agcatctccg cccaatcctg cgattccgtg ccgcccgcgc
      781 cgggatgcac ggtgcagatg gcgttgcgcg cgtcgttctc gcccgcgagc agcatctcgg
      841 tttcggcctc gccgatctcg cgttcgagcc gttcgacgag cgcgcgcagt tcccgcatgc
      901 tctccgcatc ctcacccgcc tccgcaaatt caaagagcac gcccgcgtct ccgatggcag
      961 actcgaaccc ctgctggcga ttgatgacgc gttccagacg gctgcgctgc tgcaacagac
     1021 gctgcgcctc ttcctgatcg ttccagaaat cctgcgcgga ggccagaccc tcgatgctct
     1081 ttaactcttc ctgcttcgag gcgacatcaa agaaacctcc cgagttgctc gacgcgcgca
     1141 cgcaagtctt cgtagcttgc gcgtaactct tgaactgttg aagtctcgat cattcatcct
     1201 ccaaaaaaag tgacaggtga cgagcaggaa ctaatcaatc catcgttact taaccgtcct
     1261 cttcaccgtc gctgcgatca gcaaaatgct gactgtggcg cagagggcga cgaagaggtc
     1321 gccgtaacgc gtgtaaaaag ttttgccgcc cgtcgcgcgc gagacgcgcc atgtgcgcac
     1381 ggcgggttca aagctgccgg tggcgtccaa gacctcgccg cgcggcgtga tgtgcgcgct
     1441 gatgcccgtg ttcgtcacgc gcagaacgtt gcggcggttt tcgacggcgc gaaagactgc
     1501 gtttgcgagg tgctggcgaa gcaccggagt ctggccgaga tagccgtcgt ttgagatgtt
     1561 gatgagcacg tctgcgcctg cgcctgtgaa cgtccgtgcg attgagggaa aggccgactc
     1621 gaaacagata aagactcccg cgcgcgtctc gccgagcggc agcaaagtat agtttgcgcc
     1681 cggcgtaaaa tctccgacga tgccgctcac gagccacgcg ggcggaaacc agcgcggcag
     1741 aggcacatac tcgccaaacg gcaacaggcg aatcttatca tactgtgcga cgagccgccc
     1801 cgcttcattt atcaggatcg cagagttgta tgcgccgccc gccggggcag gctcaagcga
     1861 attgaagatc acgctcgcac gcgccgcacg cgcgaacccg ccgacgagcg tttgaaactc
     1921 cgcgtcgcgc gcgtaggcga aattcatcgg cgactccggc cagacgacca ctcgcggcgg
     1981 gtcgtagata tacgcgcgcg tcccgctcga cgcgcttcgc tcaatctccc gcttcgcttc
     2041 cgcctcctgc tcctgctccc acgcgcgcag agcgtccgcg ctcatcgtca agtgacggtt
     2101 gacgagcgtg gcgttctctt ccgtagagcg tccgaagttg accgagacgt ttggctgcac
     2161 ggcgatgacg accgtataag ccgccggttc cgaactgtcg aaattgtggt ctgtaaagtc
     2221 tattgattct gtcagcgcaa acactaaccc gacggctatc agcatgagcg tggcgaggtt
     2281 gaaggcgcgc gcatttctat ggactaagac gagcgcgacc gaagcgttga cggcgacgag
     2341 cagaaagccg acggcataga caccgcccca cgtcgcgggt tgaatgagcg cgggatgaaa
     2401 ggcttgcgag tagccgacgg cgttccagag ttggccggtc gcgccgagtc gcgcccactc
     2461 cgtcgccgcc cagagacagg gcgcggcgag cagcatataa atgccccgac gcttatgcag
     2521 atgcgcgagc gcgagcgcga acgtcgcggg aaagagtccg acgatgagga cgacggggac
     2581 gagcagcgcg taggcgacgg ggcgcggcag cccgccgtag tgaatcatcg aatgggtgag
     2641 ccaccagcac gagccgtaga agaagagcgc acccgtcgtc cagccgagca cgaaggcgtg
     2701 acgcgtgcgc ggcacgcgcg ccacggccac gagcacggga acgaggccga cccatgcgag
     2761 cggccacaaa gtgaaatcgg ggaaggagag aa
//
LOCUS       DASWNS010000203         4543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_501569, whole genome shotgun sequence.
ACCESSION   DASWNS010000203 DASWNS010000000
VERSION     DASWNS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501569"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VGB76_06855"
     CDS             <1..432
                     /locus_tag="VGB76_06855"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HEY0098658.1"
                     /translation="VAFAPLGSSAVVEVEVETSVDFVRDARGRWQVARVRPGGGRAFE
                     AFDAFERALNAEKLARARADLAALAAALEAYRSARGSYIVSESASDLTDQLNPRYTAA
                     FIRFDPWHRPYEYEGTRERFTLRSLGADGKANTLDDVTVGK"
     gene            694..2505
                     /locus_tag="VGB76_06860"
     CDS             694..2505
                     /locus_tag="VGB76_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098659.1"
                     /translation="MLTGRKLLLADDSLTIQKVVSLTFNDEAMEVLTVGSGAEALEKL
                     QESLPDIVLADVFMPGPNGYELCDRIKSDARTRHLPVVLLVGTFEPFNEAEARRVGAD
                     EILTKPFQSIRDLVNKVGGLLGGSDEKSSPEARDEQSAEETTQDLSPFAADARAANPN
                     IETAAAATSTTAADAFNDFDMDDATIQSTSAAAFDARAGQTAPAALEIREEEQFSDRG
                     ETDEAFVLGLDESPTQTHAPAANTAALASGNSYSSASNASTEGAPVASAASSFSTHAA
                     RAAEADDALLELSDDIAPAHRSDALAIEDDDFKMGETRASAADDSLLELDDIAPARSG
                     EADDFILDMGDEDSFAPVAAAPTNYATPPEHDAVAEVDMLFAENAPLTAAPEAFAEPE
                     AFNAPHALTEPEVSAAPHAPAASEAWGDAEMWASPAATVAESEPTLLAEEPEAFDAPE
                     ISASPSLLFANEPAVESRGAESFAEQESERYATHALAWDGEPVAASIVAHAGSVARVE
                     SYDESQLSEPLAADASAAAVAAGASSINASPESSHAASSTSAPQLSPEALDEVVRRVV
                     AHISEQVLREIAWEVVPELAERLIKQRLEEERTRTTP"
     gene            2737..>4543
                     /locus_tag="VGB76_06865"
     CDS             2737..>4543
                     /locus_tag="VGB76_06865"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY0098660.1"
                     /translation="MEIPKTYDPKQAEERHYASWEAQGCFAPEINQDPGAPVYSIVIP
                     PPNVTGSLHMGHALQHTLMDVLTRFKRMRGFRTLWVPGEDHAGISTQIVVERELKKEK
                     LTRHDLGREKFLERVWQWRDESGSRIMQQMRREGASVDWSRQRFTMDEGLSRAVREFF
                     VRCYEENLIYHGEGMINWCPKDQTALSDLEVDKEAQAGKLYYLQYPVKGSDGRRVTVA
                     TTRPETMLGDTAVAVSPADERYLDLVGATLLLPLTEREIPVVADEFVDPAFGTGAVKV
                     TPAHDPNDYAMGLRHELPLVKVIDQHARMTGEAGAEFEGLDRYEARERVVAKFAELGL
                     LEKVVDYEFSISKCGRCRTVIEPLISLQWFCRQSELARRPLDLMRERHEPRFVPENPY
                     EKVYTNWLENLHDWTLSRQLWWGHRIPAWYTEDGRTIVARSEEEARERAGTDKLTQDP
                     DVLDTWFSSALWPISTLGWPDETKDLKTFYPTSVLITARDIIFLWVSRMMMTGMKFTG
                     EKAFADVFITGTIFDKHGQRMSKSKNNGVDPLEIFDKYGVDATRLVLASIETTDTRWN
                     ENQVESYRNFANKIWNAARFCLMNSEGARVGEGFDE"
BASE COUNT          927 a         1408 c         1400 g          808 t
ORIGIN      
        1 gtggcgtttg cgccgctcgg ctcgtctgcg gtggtcgagg tggaagtgga aacgtctgtg
       61 gatttcgtgc gcgatgcgcg cggtcggtgg caggtggcgc gcgtgcgtcc cggcggcggg
      121 cgcgcattcg aagcgttcga cgctttcgaa cgcgcgctca acgcggaaaa gctcgcacgc
      181 gcacgcgccg acctcgcagc tctcgccgcc gcgctcgaag cgtatcgaag cgcacgcggt
      241 tcctacatcg tctccgaatc ggcgtctgat ctgacagacc aactcaaccc gcgctacacc
      301 gccgccttca tccgcttcga cccctggcat cgtccttacg agtacgaagg cacgcgcgaa
      361 cgcttcaccc tgcgctccct cggcgcagac ggcaaagcga acaccctcga cgacgtgacg
      421 gtgggaaaat gacgggcggg gaatgacgag tgatgaattg aaaacagccg ctccgttttt
      481 aattcatcat tcatcattcc gccttcatcc tttcttcttg acgctttatc cgcgtctctg
      541 ttagcttcaa atagtggcct gtttgcagaa gggcaggccg gttttgaagt ctcacgcccc
      601 gcgacgtttg caccgctccc gctcgcttta aaacgcgaca gcgaggtgag ccggttgcca
      661 actgagcgac gcccccagca aggagtctgg tttttgctaa cgggacgcaa attgctgctc
      721 gccgacgatt cattaaccat tcaaaaggtg gtgagtttga ccttcaacga tgaagcgatg
      781 gaggtcttga cggtcggcag cggagccgag gcgttagaga agctacagga gagtttgccg
      841 gacatcgtcc tcgccgatgt gttcatgccg ggaccaaacg gctacgaatt gtgcgaccgc
      901 atcaagtcgg acgcgcgcac gcggcatctt ccggtggtgc tgctcgtcgg cacgtttgaa
      961 cctttcaacg aagcggaggc gcgcagagtc ggcgcggacg aaatcttgac caaacctttt
     1021 caatccatac gcgaccttgt caacaaagtc ggcggcctgc tcggcggctc cgacgaaaaa
     1081 tcgtccccag aggcgcgcga tgaacagagc gcagaggaaa cgacgcagga cttatcgccc
     1141 ttcgccgccg acgcacgcgc tgccaatcca aatattgaaa ccgccgcagc agcgaccagc
     1201 actactgccg ccgatgcctt caacgatttc gacatggacg acgccaccat ccagagcact
     1261 tccgccgccg ccttcgacgc acgcgccggg caaactgcgc ccgccgccct tgagattcga
     1321 gaggaagagc aattcagtga tcgcggcgaa acggacgaag cgttcgtctt aggtctggac
     1381 gaatcgccca cgcagacaca cgcccctgcc gccaacactg ccgctctggc atcgggaaat
     1441 tcatactcgt ctgcttccaa cgcttcgacg gaaggtgcgc ccgtcgcctc cgccgcgtca
     1501 tctttctcca cgcacgccgc acgcgccgcc gaagccgacg atgcgttgct cgaactcagc
     1561 gacgacatcg cgcccgcaca cagaagcgac gcccttgcta tcgaagacga cgattttaag
     1621 atgggcgaga cgcgcgcctc cgccgcagac gactcgctcc tcgaactcga cgacatcgcg
     1681 cccgcgcgca gcggcgaagc cgacgatttt atccttgaca tgggcgacga agattcgttc
     1741 gcgccggttg cagccgcgcc cacaaactac gccacgccgc cggaacacga cgctgtggcc
     1801 gaagtcgaca tgctcttcgc ggagaacgcg ccgttgacgg ctgcgcccga agcgttcgca
     1861 gagccggaag cattcaacgc gccgcacgcg ttgacggaac cggaagtgtc cgccgcccca
     1921 cacgcgcccg cagcatcaga ggcgtggggc gacgcggaga tgtgggcgtc gcccgctgcc
     1981 acggtcgcag agtccgaacc cactttgctc gcagaagagc cggaggcatt tgacgcgccg
     2041 gagatttcag cgtcgccttc tttacttttc gccaacgagc ctgcggtgga atcgcgcggc
     2101 gcggaaagtt ttgcggagca ggagagcgag cgctatgcga cacacgcgct cgcttgggac
     2161 ggagagcctg tggctgcgag catcgtggcg cacgcgggca gcgtcgcccg cgtcgagtcg
     2221 tacgacgaga gccaactgtc ggagccgctt gcggctgacg ctagtgcggc ggcagtggcc
     2281 gcaggagcga gttcaatcaa cgcatcgccg gaatcgagcc acgctgcgtc gtccacttct
     2341 gcgccgcagt tgtcgccgga agcattggat gaggtggtgc gtcgcgtcgt cgcgcatatc
     2401 tcggaacagg tgttgcgcga gatcgcgtgg gaggtcgtcc ccgaactggc cgaacggctc
     2461 atcaagcaac ggctcgaaga ggaacggacg cgcacgacgc cctgacagca cgcgaaccag
     2521 cgagtagcgc atgaacggat cgagttgcgg cggatgagag ttgacccgcc aaactcgatc
     2581 cgttttttct ttagctgcgc gcgattggct gtgagccatc agccgaagtc gaaaaccgcc
     2641 gccgattcct ttacaatgct tccgagcaca aatttaacgt cactgaatgc taaaagccga
     2701 aggccattag ctcaccgcta attgctcatt gaacagatgg aaattccgaa aacttacgat
     2761 ccgaaacagg ctgaagaacg ccattacgcg agttgggagg cgcaggggtg ctttgcgccg
     2821 gagatcaatc aagaccccgg tgcgcccgtc tattccatcg tcatcccgcc gccgaacgtg
     2881 accggatcgc tccacatggg gcacgccctc caacacaccc tgatggacgt gctcacacgc
     2941 ttcaagcgca tgcgcggctt tcgcacgctc tgggttcccg gcgaagacca cgcgggcatc
     3001 tccacgcaga tcgtcgtcga acgcgaactc aaaaaagaga aactgacgcg ccacgatctc
     3061 gggcgggaga agtttttaga gcgcgtctgg caatggcgcg atgaatccgg ttcgcgcatc
     3121 atgcagcaga tgcggcgcga gggcgcgtcc gtagactggt cgcgccaacg tttcaccatg
     3181 gacgagggct tgtcgcgcgc cgtgcgcgag ttcttcgtgc gctgctacga agagaacctg
     3241 atctatcacg gcgagggcat gatcaactgg tgcccgaaag atcagaccgc gctctccgac
     3301 ctcgaagtgg acaaggaagc acaggcgggc aaactctact acctgcaata tccggtgaag
     3361 ggcagcgacg gtcgccgcgt gacggtggcg acgacgcggc ctgagacgat gctcggcgac
     3421 actgccgtcg ccgtcagccc ggcggacgaa cgctacctcg acctcgtcgg cgcgacgctc
     3481 ctgttgccgc tcacggagcg ggaaattccc gtcgtcgcgg atgagtttgt tgaccccgcg
     3541 ttcggtacgg gcgcggtcaa ggtcacgccc gcgcacgacc cgaacgatta cgcgatgggc
     3601 ttgcgccacg agttgccgct cgtcaaagtc atagaccagc acgcgcgtat gacaggggaa
     3661 gcaggcgcgg agtttgaagg tcttgaccgc tacgaggcgc gcgaacgtgt ggtcgcgaag
     3721 tttgcggagt tggggctgct ggagaaggtg gtggattacg aattctcgat ctcgaagtgc
     3781 gggcggtgtc gcacggtgat cgagcctctg atctctctgc aatggttctg tcgtcagtcg
     3841 gaactcgcgc gccgcccgtt ggacttgatg cgcgagcggc atgagccgcg cttcgtgccc
     3901 gaaaaccctt acgagaaagt ttatacgaac tggctcgaaa atttgcacga ctggacgctc
     3961 tcgcgccaac tctggtgggg gcatcgcatt cccgcctggt acacggagga cgggcgcacc
     4021 atcgtcgcac gctcggaaga agaggcgcgc gagcgggcgg gcacggacaa gttgacgcaa
     4081 gacccggacg tgctcgatac gtggttctcc tccgcgctgt ggccgatctc gactctgggc
     4141 tggcctgacg agacgaaaga cctgaagacg ttctatccca cgtccgtcct catcacggcg
     4201 cgcgacatca tcttcctgtg ggtgtcgcgg atgatgatga cgggcatgaa gttcacgggc
     4261 gagaaagcgt ttgcagacgt gttcatcacg ggcacgatct tcgacaagca cgggcagcgc
     4321 atgtcgaagt cgaagaataa cggcgttgac cctctggaga ttttcgacaa gtacggcgtg
     4381 gacgcgacgc gcctcgtgct cgcttcgatt gagacgaccg acacgcgctg gaacgaaaat
     4441 caggtcgaat cctatcgcaa cttcgccaac aaaatctgga acgccgcgcg cttctgcctg
     4501 atgaactccg agggggcgcg cgtcggcgaa gggttcgacg aga
//
LOCUS       DASWNS010000204         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_476688, whole genome shotgun sequence.
ACCESSION   DASWNS010000204 DASWNS010000000
VERSION     DASWNS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476688"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..633
                     /locus_tag="VGB76_06870"
     CDS             178..633
                     /locus_tag="VGB76_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY0098661.1"
                     /translation="MIDAQQNETAEQLLRRTKVEVAPATFVLVGMRHEDWSRLLEQPE
                     LSPRPAAPFMLLRDGYEVTLLLEEEDWLTMRHAVRDARVEGGWRLLTLNIELGWNVTG
                     FLARVTEILAAAHVPLGALTAFSRDHLLIKQDDLGRALRVLGEHVAELC"
     gene            650..1168
                     /locus_tag="VGB76_06875"
     CDS             650..1168
                     /locus_tag="VGB76_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098662.1"
                     /translation="MDWIGLVNALASVATAVGVLLAGWQIRLAKQASRSQFEDSLTQQ
                     YREIIKKIPIEALLGEDLKTLEPQKWENTLDDFYRYIDLSNEQVFLRQNNRVSKATWR
                     LWSEGIKLKLELKSFKDAWEHFKREAPESFKELQLIELKDNFKSDPREWGTNWEEQLR
                     SLKAKSGKQLDR"
     gene            1146..1919
                     /locus_tag="VGB76_06880"
     CDS             1146..1919
                     /locus_tag="VGB76_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0098663.1"
                     /translation="MGSNWTDKVILITGGSSGIGEALSVEFGRRGASVGLLARREEVL
                     LEVARKVETAGGRACAVAVDVRDAGAVRAAVQTVSERFGRVDVLVANAGIGELTLARE
                     FDIEVVTQVLSVNVLGAMNSVAAVLPEMLARGSGQIVGISSLAAFRGFPGSGAYCASK
                     AALSTFFESLRAELRPRNIYVTTIHPGFIDTPMTRGRNGKMPFLLDAGRAARQMVRAV
                     EARRRTYAFPWQLATIARIGRWIPDALYDRMLAKATLRE"
BASE COUNT          427 a          585 c          635 g          395 t
ORIGIN      
        1 gcgcccggtg atcgtcccgg tcgtcgtgca ggtttagcga ccggaaaatt atttgcgcct
       61 tccgttttaa ctttgcgcct ccggcgagaa atagagttca cgccggaggc gcaaagaagt
      121 taagaccgga agcgcaaaga atttttttcc acccaccgga taccggatca caatctgatg
      181 attgacgcac agcaaaatga gacggcggag caactcctcc gccggacgaa ggtggaagtt
      241 gcgcccgcga ctttcgttct cgtcggcatg cgccacgaag attggtcgcg cctgctcgaa
      301 caacccgaac tcagcccgcg ccccgccgca cccttcatgc tcctgcgcga cggctacgaa
      361 gtgacgctcc tgctcgaaga agaagactgg ttgacgatgc gccatgccgt ccgcgacgcg
      421 cgcgtcgaag gcggctggcg actcctgacg ctcaatatcg aactcggctg gaacgtgacg
      481 ggctttctgg cgcgcgtcac agagatactg gccgccgctc acgttcccct cggcgcgctg
      541 acggccttct cgcgcgatca cctgctcatc aaacaggacg atctggggcg tgctctgcgc
      601 gtgctcggcg aacacgtagc ggaactctgc tgacgcgagg ggcgggttca tggattggat
      661 aggtctggta aacgcgctcg cctctgtcgc cactgcggtc ggcgtgttgc tggcaggctg
      721 gcagattcga ttggcgaaac aggcgtcgag gtcgcaattt gaagatagcc tgacgcagca
      781 gtatcgagaa ataatcaaaa agattcctat cgaagcgttg ttgggagaag accttaaaac
      841 gcttgagccg cagaaatggg aaaatacgct tgacgatttt tacaggtaca ttgacctgtc
      901 caacgagcaa gtgtttcttc gccagaataa ccgcgtgagc aaagctacct ggcggttatg
      961 gtctgagggc atcaagttga agctggaact gaaatctttc aaggatgctt gggagcattt
     1021 caagcgggaa gcgcccgaat ccttcaaaga acttcaactg attgagttga aagataactt
     1081 caaaagcgat ccgcgcgagt ggggcacaaa ctgggaagag caattgagat cgctaaaagc
     1141 caaaagtggg aagcaactgg acagataagg tcatcctcat cacgggcggg tcgagcggca
     1201 tcggcgaggc tctgtccgta gagttcggtc ggcgcggggc gtcggtcggg ttgctggcgc
     1261 ggcgtgaaga ggttttgctt gaggtcgcgc gtaaagttga aaccgccggg ggaagggcat
     1321 gcgccgtcgc ggttgacgtg cgcgatgcgg gagcggtgcg cgcggcggta cagaccgtca
     1381 gcgagcggtt cgggcgcgtt gacgtgctcg tcgccaacgc gggcatcggc gagctaacgc
     1441 tggcgcggga gttcgacatc gaagtcgtga cgcaggtgct ctccgtgaac gtgctcggcg
     1501 cgatgaactc ggtggcggcg gtcttgccgg agatgcttgc gcgcggcagc ggacagatcg
     1561 tcggcatctc aagcctcgcg gcctttcgcg gctttcccgg ttccggcgcg tactgcgcga
     1621 gcaaggccgc gctctccacg ttttttgaga gtttgcgcgc cgaactgcgc ccacgcaata
     1681 tctacgtcac gaccattcat ccgggcttca tcgacacgcc gatgacgcgc gggcgcaacg
     1741 ggaagatgcc gtttctgctg gacgccggac gcgccgcgag acagatggtg cgcgccgtcg
     1801 aagcgcgacg ccgcacctac gccttcccgt ggcaactcgc caccatcgca cgcatcggtc
     1861 gctggattcc cgacgcgctc tacgaccgca tgctcgccaa agccacgctc cgcgagtaaa
     1921 aataaggccg cgctcagcgc atcaaacata atgaacatct ttcaagccat catcatcggc
     1981 atcgtgcagg ggttgaccga gtttatcccg atcagttcga cggctcattt gatcttcgcc
     2041 gc
//
LOCUS       DASWNS010000205         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_112947, whole genome shotgun sequence.
ACCESSION   DASWNS010000205 DASWNS010000000
VERSION     DASWNS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112947"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VGB76_06885"
     CDS             <1..1138
                     /locus_tag="VGB76_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEY0098664.1"
                     /translation="LEAHCFRNLSGKVAWGAGLNLIYGDNGQGKTNWLEAIYLLATSK
                     SFRTQKPQEAVRFDEELAVVRGRVARSEEVQRDLQVTIQNNIKTLTVNGKRESVARYL
                     GQLHVVAFTADELEVVRGAPEARRKFLDRGIVSLHHAYVQTISDYQRVIKQKNRLLQD
                     AAEAELGAVETAARLAPWNEQLVVLSTAIHQARTDYVERLNAVLERRLFGREEVTMRY
                     VSSLEGKGDLSDYQTLIAERLQFRMPAEMSAGYSLIGPHRDELEILFDGRDIRSFGSS
                     GQQRSALIILDLAAISVYHSWHNEYPVFLLDDVDAELDRKRIDQLLEYLEGRTQTFVT
                     TSKESLVEQYGARARIFEVRDGAASLSNTPESQISTSSKGASSI"
     gene            1222..>3456
                     /locus_tag="VGB76_06890"
     CDS             1222..>3456
                     /locus_tag="VGB76_06890"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEY0098665.1"
                     /translation="MSTAINTTKKIPSYDSSSITVLEGREAVRKRPAMYIGSTGDIGL
                     HHLVYEVVDNSIDEALAGYCDTVDVTIHLDNSITVIDNGRGIPVDYHKGEKKSAAEVV
                     MTKLHAGGKFDSNAYKVSGGLHGVGVSCVNFLSEWLRLEIWREGATYEQEYKQGIPVA
                     KLEATGKTRKRGTKITFKPDSSIFDVSEFSFDKLSERLREKAFLNKGIRITIRDEREE
                     TERSHEFYYKGGIAEFVKHLNKNKTVLHDKPLYFERVADALSVEVAIQYNDAYDEKVY
                     SFANNINTVDGGTHLSGFRSAFTRTINAYAQSSGLAKNFKTSLTGDDVREGLVAVISV
                     KLPQPQFEGQTKGKLNSDVKGAVESFLNERLTEFFEQNPTVAKRIVGKAIDAARAREA
                     ARKAREIVRKGVIGSTMLPGKLADCQERDPTLCELYIVEGDSAGGSAKQGRDRKNQAV
                     LPLKGKILNVEKARFDKMLSHTEIKTLITALGTGIGKDDFDLGRLRYHKIIFMTDADV
                     DGSHIRTLLLTFFYRQMPELVESGHVYIAQPPLFKVKRGKKEEYIKDERSMVRYLMRQ
                     ATSEAKVKSGDGVTIEGKDLARSLEQMVEYKRYCEKLVRRLGNDERLLSTLLNAFAGR
                     KGVLRREKSSNLRSIFQKESRELLVAVEGQLAKAGYRTDLLTDEEHGLLSIETVAASG
                     TKVLVDWNLASHVEFQKSVELHKVLDESLAAPFTFGENGTSEQVETREALLERVLATA
                     KKDLS"
BASE COUNT          832 a          973 c          988 g          663 t
ORIGIN      
        1 acttgaagca cactgtttca gaaatttgag cggcaaagtc gcctggggcg cgggactcaa
       61 cctcatctac ggcgataacg gccagggcaa gacaaactgg ctggaagcca tctacctgct
      121 cgccacgtct aaatccttcc gcacacagaa accgcaggag gccgtccgtt tcgacgaaga
      181 actggccgtc gtgcgcgggc gggtcgcacg aagcgaagaa gtccaacgcg acctacaggt
      241 cacgattcag aacaacatca agacgctcac cgtcaacggc aaacgcgagt cggtcgcgcg
      301 ttatctggga caactccacg tcgtcgcttt tacggcagac gagttggagg ttgtgcgcgg
      361 cgcgcccgaa gcccggcgca agtttctgga tcgcggcatc gtctcgctgc accacgccta
      421 cgtgcagacg atctccgact atcagcgcgt catcaaacag aaaaaccgcc tgctgcaaga
      481 cgccgccgaa gccgaactcg gcgcagtgga aaccgccgcg cgtctcgccc cctggaacga
      541 gcaactcgtc gttttgagca cggcaatcca ccaggcgcgc acggactacg tcgagcgtct
      601 caacgccgtc ctcgaacgcc gtcttttcgg gcgcgaggaa gtgactatgc gctacgtttc
      661 atcgctcgaa ggaaagggcg atttgagcga ctaccagacg ctcatcgccg agcgtctgca
      721 atttcgcatg ccagcggaaa tgagcgccgg ttactcgctc atagggcctc accgggacga
      781 gttggagatc ttgtttgacg ggcgggacat acgttctttc gggagttccg gccaacagcg
      841 tagcgcattg ataatacttg acttagctgc aatttccgtg tatcattcgt ggcacaatga
      901 gtatccggtt tttttgctcg atgatgtgga tgcggagctt gaccggaagc gtatcgatca
      961 actgttggag tatctcgaag ggcgcacgca gaccttcgtg acgacctcca aagagagcct
     1021 cgtcgaacaa tacggcgcgc gcgcgcgaat cttcgaagtg agagacggcg cggcgagttt
     1081 gagcaacact cccgaaagcc agatttcgac atcctcgaag ggtgcttcga gcatctaaat
     1141 caagcgcgtt ttcagtcgtc ttctgcgaac gttctcaata gcgcaaaagt agtttttagc
     1201 ggttagaaag aggagtttag tttgtcaaca gcaatcaaca caactaagaa aatcccgagc
     1261 tacgattcaa gttcgatcac ggttctcgaa gggcgcgagg cggtgcgcaa gcgtcccgcc
     1321 atgtacatcg gctcgaccgg cgacatcggc ctgcatcatc tcgtctacga ggtggtcgat
     1381 aactcgattg acgaagcgct cgccggttat tgcgacaccg tggacgtgac gattcatctc
     1441 gacaactcga ttacggtcat cgataacggg cgcggcatcc ccgtggatta tcacaagggc
     1501 gagaagaaat ccgccgccga agttgtcatg acgaaactcc acgcgggcgg gaaattcgac
     1561 tccaacgcct acaaggtgtc cggaggtctg cacggagtag gcgtctcttg cgtaaatttc
     1621 ctctctgagt ggcttcgtct ggaaatctgg agggaagggg cgacttacga gcaggagtac
     1681 aagcagggga ttcctgtggc gaaattggaa gcgaccggaa agacgcgcaa gcgcggcacg
     1741 aagatcactt tcaaacccga ctcctcgatc ttcgatgtct ccgagttcag cttcgacaaa
     1801 ctctccgagc ggctgcgcga aaaagccttc ttgaacaaag gcattcgcat caccatccgc
     1861 gacgaacgcg aagagacgga acgctcgcac gagttctact acaagggcgg catcgccgag
     1921 tttgtcaaac atctcaacaa gaacaagacc gttctgcacg ataagccgct ctattttgaa
     1981 cgagttgccg acgcactctc ggtcgaggtg gcgatccagt acaacgacgc ctacgacgaa
     2041 aaggtctatt ctttcgccaa caacatcaac accgtcgatg gcggcacgca cctctccggc
     2101 ttccgttcgg cctttacgcg caccatcaac gcttacgcgc agtcctccgg actcgccaaa
     2161 aacttcaaga cttcgctcac cggcgacgac gtgcgcgaag gactcgtcgc ggtcatctcc
     2221 gtcaaactcc cgcaaccgca gtttgaaggc cagaccaaag gcaaactcaa ctcggacgtg
     2281 aaaggcgcgg tcgaatcctt cctcaacgag cgtctgacgg agtttttcga gcagaacccg
     2341 accgttgcca agcgtatcgt cggcaaagcc atcgacgccg cacgcgcacg cgaggcggcg
     2401 cgtaaggcgc gcgagatcgt ccgcaagggc gtcatcggct ccacgatgct ccccggcaaa
     2461 ctcgcagatt gtcaggaacg cgaccccacg ctttgcgaat tgtacattgt agaaggcgat
     2521 tcggcgggcg gcagcgcgaa acaggggcgc gaccgaaaga atcaggccgt tcttccgtta
     2581 aaaggtaaaa tcttgaacgt ggagaaggcg cgattcgaca aaatgctcag ccacaccgag
     2641 attaaaacgc tcatcaccgc gctcggaacg ggcatcggca aagatgattt cgacctcgga
     2701 aggctccgtt atcacaaaat catcttcatg acggacgccg acgtggacgg ctcgcacatt
     2761 cgcacgctgc tcctgacttt cttctaccgg cagatgccgg aactcgttga gtccgggcac
     2821 gtttacatcg cgcagccacc gttgtttaaa gtgaaacgag gtaagaaaga ggagtacatc
     2881 aaggacgagc gttcgatggt tcgctacctg atgcggcagg cgacgagcga agcgaaggtc
     2941 aagtccggcg acggggtgac gattgaaggg aaagatttag cgcgttctct ggagcagatg
     3001 gtcgagtaca agcgttattg cgagaaactc gtgcgccggt tgggtaacga cgaacgcttg
     3061 ctgtccaccc tcctgaacgc cttcgccggt cgtaaaggtg tcttgcggcg tgaaaaaagc
     3121 tcgaaccttc gaagcatctt ccagaaagag agtagagaac tgctggtcgc cgtcgagggg
     3181 caactcgcca aagcgggcta ccgcacggat ttactgacgg atgaagagca cgggttattg
     3241 tcaatcgaaa cggttgccgc gagcgggact aaagtgctgg ttgactggaa tctcgcgagc
     3301 cacgttgagt ttcagaagtc cgtcgagttg cataaagttc tcgacgagag tttggccgcc
     3361 ccgttcacct ttggggaaaa cgggacgagc gagcaggtcg agacgcgcga ggctctactt
     3421 gagcgggttc tggctacggc gaagaaagac ctgagc
//
LOCUS       DASWNS010000206         9099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_191076, whole genome shotgun sequence.
ACCESSION   DASWNS010000206 DASWNS010000000
VERSION     DASWNS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9099
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191076"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1013
                     /locus_tag="VGB76_06895"
     CDS             45..1013
                     /locus_tag="VGB76_06895"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY0098666.1"
                     /translation="MRFFNAVTCLTFLFIVSACNVQRGNEAQSDRTNAVSPSNGGAHS
                     DGAAKQEVAYDSKQTGMQPVSLVQASQSQAAQQVAVERKIIRDAEINLEVAAPAEGQR
                     KLASIAEAHGGFVVTSEARLEEHDSQLLEIVTVQLRVPASHFDAALAEIRAVGTRVRA
                     EKQTGKDVTEEFIDLDARLHTQRALEAQFLEIMKRAAKVSDALEVQTQLATVRMEIER
                     VEGRRRFLENQAALSTINATLRPPAALVSVNSSGFFYGIKRAFGDGIDIAAMIILGLI
                     RLGIALLPVLLLVVLPFALLWKYLLRPRFRRPKRGAPAPPVAGTAS"
     gene            1088..1657
                     /locus_tag="VGB76_06900"
     CDS             1088..1657
                     /locus_tag="VGB76_06900"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY0098667.1"
                     /translation="MRQLSTRLIFPCALLVALLSACAQNTAPTNTPAANSANAAASSN
                     ANTATHAPKSPVEGIVSASVERVEMSAGEQAETSVRLSIKDGYHVNANPATEKFLIPT
                     VLSVKPEAGITVDKIDYPKSLKRTFPYSKAPLDIYEGDAVIKLTLNAPRDLKPGEHTL
                     RTDIRVQPCDHQTCYPPGTIATAIAVTIR"
     gene            1664..1942
                     /locus_tag="VGB76_06905"
     CDS             1664..1942
                     /locus_tag="VGB76_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098668.1"
                     /translation="MPITNETEESTHTPESLRARLSRQRWSIAAGVCLFIALLLLLSW
                     RIDAAFVVATLGVVAWFWNERNRLLPHGIEARERFTDEDEEDEVGDEK"
     gene            1920..2525
                     /locus_tag="VGB76_06910"
     CDS             1920..2525
                     /locus_tag="VGB76_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098669.1"
                     /translation="MKLEMKNRFLLFSGRLFVAIFLLALMASTTTLFAAPVTTARVAP
                     NEAEVRSTVERAFQQLKSGDYNSLYDVLPSASQRRLSREQFVRALERTRGMYELDRVE
                     IGAVRVSGDLGVVDSVVYGRGRAPFAGEAKIVARQYLVREGGRWRVTTGDRATIQPLL
                     TANPAFARRYPLTQPRLYLKRDNRWVDMSKELQGMRRRASK"
     gene            complement(2587..2859)
                     /locus_tag="VGB76_06915"
     CDS             complement(2587..2859)
                     /locus_tag="VGB76_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0098670.1"
                     /translation="MEEASSHREKREEFLTARQLAAILQVSESTVRRLAREGRIPAIR
                     LTSRLLRFHLQSVMSALDGTQAKTRTRRAPPHEATADPQLSFEDLM"
     gene            complement(2882..3511)
                     /locus_tag="VGB76_06920"
     CDS             complement(2882..3511)
                     /locus_tag="VGB76_06920"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0098671.1"
                     /translation="MSLNRHKFIPALFLLMLLAASLAAQKNGTTGSIKGKIKTESGSA
                     EGVRVTVRQGEREVATATTDRKGGFVIAGLAAGSYSLTFRKPGLRVAEIKDFQVVAGK
                     SRSLGSDLMLPADEGSLVFVKGGVFTEEGRSLRGARVELARVEADGTIKKLDGRISNE
                     SGSFTFRLPVETARYRVTAKMDGMEAASKDVEVEGAAVYRVALSLKPAP"
     gene            3814..4944
                     /locus_tag="VGB76_06925"
     CDS             3814..4944
                     /locus_tag="VGB76_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0098672.1"
                     /translation="MLYPRKISSAAVVVAAPIARGRVRNGLLAVCVVAFAALSGSLPV
                     AQSQQPQPQTETPPQQQQQRPRRVTPVPSGAQKEDDTDEVLRVDTDLVLVDVTVTDAS
                     GRPVRNLKAEDFKVYDEGVERPIAFFNVERKSGTPRPVAIVFAVDVSGSMTPEEMERL
                     ASAMRLFLRRFSDRTSLFAVMTFGMSARVLQPFTNDAEKLERALARLAREPNGLSTHT
                     YDAVDDAIRLLVRHAPRTRDRRLMKRAVVVVTDGFPVGDTVAPKTVIERANAAEVSVY
                     TVTLPSFSRLFSSRGGAANAHAPLPTPLDVSELAEKTGGSNAYATEKDFGPLFQALAE
                     EVTSSYILAFYPPEEKRRDGRFHPLRITAPPGLNVRQSRPGY"
     gene            complement(5090..5275)
                     /locus_tag="VGB76_06930"
     CDS             complement(5090..5275)
                     /locus_tag="VGB76_06930"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098673.1"
                     /translation="MPTGTCPECDADVHVDTDADKGDAVACEECGTELEIVGLDPVEL
                     DIVEDDELDDDDEDDEF"
     gene            complement(5466..6194)
                     /locus_tag="VGB76_06935"
     CDS             complement(5466..6194)
                     /locus_tag="VGB76_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098674.1"
                     /translation="MHLSLAAWACLLLLVAADAYPQRKRRAPPAGGQHAVVVDERLAA
                     LRDEAKLTARLVQRLGRGRTVSITGARRGGDGFNFYRVAVTRRTRGWLPAESVAAPTR
                     AGDDERLVRLIRGSEEFARIARARIFLDYFPRSPLRPAVLMLFGDEAEAAASKLSRDA
                     ARRLDAGEMAAGGAPFDSYFLNFNELDRYNKQGVTFVFERAARRFHYDGASWREILRR
                     YPRSPEATEARKRLDALAAFAVSR"
     gene            complement(6368..6922)
                     /gene="ruvC"
                     /locus_tag="VGB76_06940"
     CDS             complement(6368..6922)
                     /gene="ruvC"
                     /locus_tag="VGB76_06940"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY0098675.1"
                     /translation="MRVLGIDPGSETTGWGVVEGDARTYRAVDFGTLKASPRERFAAR
                     LLKISDGMEELLTRFRPDVCSVEEAFFAVNVKTALKLGQVRGVILVAAERAGVEIAEY
                     APRLVKQTVVGYGAAEKHQVQEMVRVLLRLASIPQPHDAGDALALAICHFHHGGLAAR
                     LKPTAARSKLDALLAANPRRRAPR"
     gene            complement(7065..7802)
                     /locus_tag="VGB76_06945"
     CDS             complement(7065..7802)
                     /locus_tag="VGB76_06945"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0098676.1"
                     /translation="MSGHSKWHTIKHKKGALDAKRGKLFTKLIKELTVAARTGGGDPE
                     ANARLRKAVSDAKAGNMPNDTIARAIRRGTGEEEGVNYDEITYEGIGPGGVAIMIEAM
                     TDNRNRTVAEIRHAFSKNGGALGVSGSQAWNFEKKGYIVVEKAAKSEDELFEIVTEAG
                     AEDLRDDEDNFEIITAPENFEAVHEALKKAGIEPQAADVEMIPKSYVKLEGAEARSML
                     KLMETLEDHDDVQKVSANFDIDEAEMV"
     gene            complement(7939..8235)
                     /locus_tag="VGB76_06950"
     CDS             complement(7939..8235)
                     /locus_tag="VGB76_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY0098677.1"
                     /translation="MLRCTETGGALVFTVRVVPRASQSEVVGEHDGALRVRVAAPPVE
                     GAANVELARTLARALGVPVRAVEITSGHSSKTKLVRVAGAERERLLSLVNSSSD"
     gene            complement(8634..>9099)
                     /locus_tag="VGB76_06955"
     CDS             complement(8634..>9099)
                     /locus_tag="VGB76_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY0098678.1"
                     /translation="PVLFVRNLTDPFVNPVRRSLLNFGFTPNMAPLVTILIVILVGWL
                     ALSLSNDVISTFYGMFLRSREGRMVALVGTLLYGFLSVYSMLILIRIIFSWGMVGLRN
                     PVMRFLVTATDPLLVPLRRIIPPLGFFDISPLVAFLLIWLFQTAIAGTLMRF"
BASE COUNT         1796 a         2835 c         2702 g         1766 t
ORIGIN      
        1 agacgttcta aacgcagaat cctgaagcaa cagcggaggc cgacatgaga tttttcaacg
       61 ccgtcacctg tttaactttc ctgtttatcg tctccgcctg caacgtgcag agaggaaacg
      121 aagcgcagag cgaccgtacg aatgcggtca gcccgtcgaa tggcggcgcg catagcgacg
      181 gtgcggctaa gcaggaggtc gcctatgaca gcaagcagac gggaatgcag cccgtctcgc
      241 tcgttcaggc cagccagtcg caggcggcgc agcaggttgc cgtcgagcgc aagatcatcc
      301 gcgacgcaga gatcaatctc gaagtcgccg ccccggctga aggccagcgc aaactcgcct
      361 ctatcgccga agcgcacgga gggttcgtcg tcacgtccga agcgcggctg gaagagcacg
      421 actcgcaact gttggagatc gtcaccgtcc aactgcgcgt tccggcttca cacttcgacg
      481 ccgcgctcgc agagatacgc gccgtcggca cacgcgtccg cgcggagaag cagacgggca
      541 aggatgtgac ggaagaattt atcgacctcg acgcgcgcct gcacacgcaa cgcgcgctcg
      601 aagcgcagtt tctcgaaatc atgaaacgcg ccgcgaaggt gtccgacgcg cttgaagtac
      661 agacccagct ggcaacggtc cgcatggaga tcgagcgagt cgaaggccgc cgtagatttc
      721 tggaaaatca ggccgcgctc tccaccatca acgccacgct gcgaccgccc gccgcgctcg
      781 tcagcgtcaa ctccagcggc ttcttctacg gcatcaagcg cgcgttcgga gacggcatag
      841 acatcgccgc gatgatcatt ctcggcctca tccgtctcgg catcgcgctg cttccggtgc
      901 tgctcctcgt ggtgcttccg ttcgcgctgc tctggaagta tctcctgcgc ccacgctttc
      961 gtcgcccgaa aagaggtgcg cccgcgccgc ccgtcgccgg gacggcaagt tgagattccg
     1021 cgccgcacga cgcaactctt cgtctgccaa cttgagactt ccagccatca actttagact
     1081 ctccctgatg agacaacttt caacccgtct gatttttccc tgcgccctgc tcgtcgcgct
     1141 gctgtcggcg tgcgctcaaa acaccgcgcc gaccaacacg cccgccgcta actctgcaaa
     1201 cgccgccgct tcgtcaaacg cgaacacggc gacgcacgct cccaagtcgc ctgttgaagg
     1261 catcgtgagc gcgagcgtcg agcgggtcga gatgagtgcg ggcgagcagg cggagacgag
     1321 cgtgcggctc tcgatcaagg acggttatca cgtcaacgcg aatccggcga cggagaagtt
     1381 tctgattccg accgtgctca gcgtcaagcc ggaagcgggc atcacggttg acaagatcga
     1441 ctacccgaag tcgctcaaga gaacgtttcc ctactcgaaa gcgccgctcg acatttatga
     1501 aggcgatgct gtcatcaagt tgacgctcaa cgcgccgcgc gacctgaagc cgggagagca
     1561 tacgctgcgg acggacattc gcgtgcagcc ctgcgaccat cagacgtgct atccaccggg
     1621 aaccattgcg accgccattg ccgtcaccat ccgttaaaaa gctttgccaa tcacgaacga
     1681 aacagaagaa agcacgcaca cgccggagag cctgcgggcg cgtctctccc ggcagcgttg
     1741 gagcatcgcc gccggtgtgt gcctgttcat tgcgttgttg ttgttgttgt cgtggcgcat
     1801 agacgcagcg ttcgtcgtag cgacgctcgg cgtcgtcgcg tggttttgga acgagcgcaa
     1861 ccgactgctc ccgcacggca tcgaagcccg agagagattt acggatgagg atgaagaaga
     1921 tgaagttgga gatgaaaaat agatttttgc tcttttccgg caggctgttc gtcgccatct
     1981 ttttgctggc gttaatggcg agcacgacaa ccctcttcgc cgcgccggtc actacggctc
     2041 gcgtcgcgcc gaacgaagcg gaagtgcgaa gcacggtcga gcgtgccttt caacaattga
     2101 agtccgggga ttacaactcg ctttacgacg tgctgccttc ggcctcgcag aggcgtctgt
     2161 cgcgcgagca gttcgtgcgc gcgctcgaac gcacgcgcgg catgtatgag ttggatcgcg
     2221 tggagatcgg tgcggtgcgc gtctcgggcg atctcggcgt ggttgattcg gtggtttacg
     2281 gtcgcgggcg cgcgccgttt gcgggcgaag cgaagatcgt ggcgcgtcaa tatctggtac
     2341 gcgagggcgg gcgttggcgc gtcacgacgg gcgaccgcgc gacgattcaa ccgttgctca
     2401 cggctaatcc cgccttcgcc agaagatacc cgctcaccca accgcgcctc tacctgaaac
     2461 gcgataatcg ctgggtggac atgagcaagg aactacaggg catgcgccgc cgcgcgtcga
     2521 aataattcgc gcgcaataaa ccgtcaacac aaaacagccc gcgtcttcaa tcaggcgcgg
     2581 gctgcttcac attaaatctt caaaagagag ttgcggatcg gcggtcgctt catgcggagg
     2641 cgcgcgacgg gtgcgcgttt tcgcctgtgt gccgtcgagc gcgctcatca cggattggag
     2701 gtggaagcgc aggaggcgcg aggtgagacg gatggcagga atgcgccctt cgcgtgcgag
     2761 gcggcgcacg gtggattcgc tcacctgtag gatggcggcg agttggcgcg cggtcaaaaa
     2821 ttcttcgcgc ttctcccgat gtgacgaagc ctcttccatc taagcgaaac ttcccccggc
     2881 ctcagggcgc gggtttgagc gaaagcgcga cgcgatagac agccgcgcct tcaacttcga
     2941 catctttcga ggcggcttcc atgccgtcca tcttggccgt cacgcggtag cgcgccgtct
     3001 cgaccggcaa gcggaaggtg aaagagccgg actcgttcga gatgcgcccg tcgagttttt
     3061 tgatcgtgcc gtcggcctcg acgcgcgcca gttccacccg tgcgccgcga aggcttcgcc
     3121 cctcttccgt gaagacgccg cccttgacga agacgagcga gccttcgtcg gcgggcaaca
     3181 tgagatcgga gccgagcgag cgcgacttgc ccgcgaccac ctgaaaatct ttgatctccg
     3241 ccactctcaa gcccggcttg cgaaacgtga ggctgtaact gcccgccgcg agtcccgcga
     3301 tgacaaagcc gcctttgcgg tcggtggtcg ccgttgccac ttcgcgttcg ccctgtcgca
     3361 ccgtcacgcg gacgccttcc gccgagccgc tctcggtctt gatcttgccc ttgatgctcc
     3421 ccgtcgtccc gttcttctgc gccgcgaggc tcgcggccag cagcattaat aaaaagaggg
     3481 cggggatgaa tttatgtctg ttcaaactca tgtcttgaaa tctctcgtgc gaactctctt
     3541 tagccgggcg cggcctctgc cttcgtgcag agggcgtgga ttatacatag aatggggcgg
     3601 tgcatgttac acgcgccgtt tgcgtgcctt ccagttgatg cggcgcaggc ggctctctgt
     3661 tgccctgcgg acggacggag gggcgtctgc cgggtgagat gacacgggca cgccggactc
     3721 cgtataatgc acgcactccc ggcgcgcgtt tgctgaaaca atacgcgact gaacgacgtt
     3781 gaatttctcc tgattagttc gcttccgagt tttatgttgt acccccgcaa gatttcgtcc
     3841 gccgctgttg tcgtcgctgc cccgatagct cgcgggcgcg tgagaaacgg gctgctcgcc
     3901 gtgtgcgtcg tcgccttcgc agcgttgtcc ggttccctgc ccgtcgcaca aagccagcag
     3961 ccgcaaccac agaccgagac gcccccacag caacaacaac aacgcccgcg ccgcgtcacg
     4021 cccgtcccct ccggcgcgca gaaagaggat gacacggacg aagttctgcg cgtcgataca
     4081 gacctcgtgc tcgtggatgt caccgtgacg gacgcctccg ggcgacccgt gcgcaacctc
     4141 aaggcggagg atttcaaagt ctacgacgaa ggcgtcgaac gccccatcgc cttcttcaat
     4201 gtcgagcgga agagcggcac gccgcgcccc gtcgccattg tctttgcggt ggacgtgtcg
     4261 ggcagcatga cgccggaaga gatggagcgg ctggcgagcg cgatgcgcct tttcctgcgt
     4321 cgcttctccg accgcacctc gctctttgcc gtgatgactt tcggaatgag cgcgcgcgtg
     4381 ctgcaacctt tcaccaacga cgcggagaaa ctcgaacgcg cgctcgcgcg tctggcgcgc
     4441 gaacccaacg gactctccac gcacacttac gatgcggttg acgacgccat ccgcctgctc
     4501 gtgcgccacg cgccgcgcac gcgcgaccgc cgtttgatga agcgcgcggt ggtggtggtg
     4561 acagacggtt ttccggtggg agatacggtc gcgccgaaga ccgtcatcga acgcgccaac
     4621 gcggcggaag tgagcgtcta tacggtgacg ctgccatctt tctcgcgcct cttcagttcg
     4681 cgcggcggcg cggcgaacgc acacgcgcct ttgcccacac ccctcgatgt cagcgagttg
     4741 gcggaaaaga ccggcggctc aaacgcttat gcgacggaaa aagatttcgg gcctctcttt
     4801 caagctctgg ccgaagaggt gacatcttct tacatactgg ccttctaccc gcccgaagag
     4861 aaacgccgcg acgggcgttt ccacccgttg cgcatcacgg ctccgcccgg cctcaacgtc
     4921 cgccagagcc gccccggata ttgaaagacg gcgacgagtg acaagcgacg agtcagaaca
     4981 aaatccgtga atcgtgaatc gtaaatagag aaaagcagag gtctttctat tcacgattga
     5041 cgattcacgg attttctcgt cacttgtcac tcgtcacttg tcactgcttt taaaactcgt
     5101 cgtcttcgtc gtcgtcatct aattcgtcgt cttcgacaat gtcgagttcg acgggatcga
     5161 gtccgacgat ctccagttcc gtaccgcatt cttcgcacgc gacagcatca cccttatcgg
     5221 cgtctgtatc gacgtgcaca tccgcatcgc attcggggca tgttccggtt ggcacttcgt
     5281 ctctacctcc agcgcattca ggaggagcgc ccttcctgtc tggggagacg tccaccttga
     5341 aataattttg gttgaaccgc cgacaatcta accgcgcccg ccgttttttg caacactcaa
     5401 ttttcactct cccgaagtta tgcgcccatc ctaaattttg ccgcgagaat acaggggaaa
     5461 agagcttacc ggctgacggc aaaagcggcc agcgcgtcga gacgtttgcg cgcctccgtc
     5521 gcttccgggc ttcgcgggta gcgacgcaga atctcacgcc agctcgcgcc gtcgtaatga
     5581 aaacggcgcg cggcgcgttc aaagacgaac gtgacgccct gcttgttgta acgatccagt
     5641 tcgttgaaat tcagaaagta gctgtcaaac ggtgcgccgc ccgccgccat ctcgcccgcg
     5701 tcgagtcgcc gcgcggcatc gcgcgacagt ttcgacgctg ccgcctccgc ttcatccccg
     5761 aagagcatca agaccgccgg acgcaagggc gagcgcggaa agtagtcgag aaaaatccgc
     5821 gcccgcgcga tgcgcgcaaa ctcttccgag ccgcgaatca ggcgcaccaa acgctcatca
     5881 tcgcccgcgc gcgtgggcgc agcgacgctc tcggcgggca gccagccgcg cgtcctccgc
     5941 gtcaccgcga cgcgataaaa gttgaacccg tcgccgccgc gacgcgcgcc cgtgatcgag
     6001 actgtgcgcc cgcgaccgag acgctgcacg agtcgtgccg tcaacttcgc ttcgtctctg
     6061 agcgcggcca aacgctcgtc aaccacgacg gcgtgttgcc cgcccgcagg cggagcgcgc
     6121 cgcttccgtt gcgggtaagc gtccgccgcg accaacagca gcaggcaagc ccacgcagcg
     6181 agactcaaat gcatggagcg cgcacgcggc gcaacgctcc tgcgaacgga acttcgtttg
     6241 aggttccggc ggatggaact tcgtctaagg ttgaatgaga attgagacat gaagaatcgc
     6301 tctcctttcg cttcgtcgaa gctgaagaaa agcaaaaccg gaggcggcac cgtcgcaggt
     6361 tgcgctttta acgtggagcg cggcggcgcg ggtttgcggc taacagggcg tcgagtttgc
     6421 tgcgtgcggc ggtgggtttc agccgcgcag caagcccgcc gtgatggaag tggcagatag
     6481 cgagcgcgag ggcgtcgcct gcgtcgtgcg gttgcgggat gctcgccaga cggagcagca
     6541 cgcgcaccat ctcctgcact tgatgtttct cggcggctcc gtagccaacc accgtctgct
     6601 tgacgaggcg cggcgcgtat tccgcgatct ccacgcccgc gcgctccgcc gctaccagga
     6661 tcacaccgcg cacctgcccg agtttcagag cggtcttgac gttcacagcg aagaacgctt
     6721 cttcaaccga gcacacgtcc gggcggaagc gcgtgagcaa ctcttccatc ccgtcgctga
     6781 tcttcaacag gcgcgccgcg aaacgctcgc gcgggctggc cttgagcgtc ccgaaatcaa
     6841 ccgcgcgata ggttcgcgca tcgccctcga ccacgcccca gcccgtcgtc tcgctccccg
     6901 gatcaatacc aagcacacgc atttaaaaac agtgacaggt gacaggtgac aagttaaaac
     6961 agcgacaagt gacgggtgac aggtgacgag atgaaagaca agttttaaga caggttttct
     7021 tctgctttaa cttgtcacct gtcacctgtc actcgtcact gctttcagac catttccgct
     7081 tcgtcaatgt cgaagttggc ggagactttt tgcacgtcgt cgtggtcttc gagagtctcc
     7141 atcaacttga gcatggagcg cgcttccgcg ccttcgagtt tgacgtagga tttcggaatc
     7201 atctcgacat cggcggcctg cggttcgatg ccagccttct tcagcgcttc gtgcaccgct
     7261 tcgaaattct ccggcgcggt gatgatctcg aagttgtctt cgtcgtcgcg cagatcctcc
     7321 gcgcccgctt cggtcacgat ctcgaagagt tcgtcttcac tcttcgccgc tttctcgacg
     7381 acgatgtagc ccttcttctc gaagttccat gcctgcgaac cggagacgcc gagcgcgccg
     7441 ccatttttag aaaaggcgtg gcgaatctcc gccaccgtgc gattgcggtt atccgtcatc
     7501 gcctcgatca tgatggcgac gccgccgggt ccgatgcctt cataagtgat ctcgtcatag
     7561 ttcacgccct cttcttcgcc cgtgccgcga cgaatagcgc gtgcgatggt gtcgttgggc
     7621 atgttgcccg ctttggcgtc ggagacggct ttgcgcaggc gcgcgttggc ttcggggtcg
     7681 ccgccgcccg tgcgcgcggc aacggtgagt tctttaatga gcttggtaaa aagtttgccg
     7741 cgcttggcat cgagcgcgcc cttcttgtgt ttgatggtat gccacttgga atgtccagac
     7801 atttttaaag agttccttgt acgtgtgttt gatcttgaag cggaaggcga ctatatcacg
     7861 cgttttttac gcgccgcaac cgggtcaaag cagaggttgg aggccagagg tcggagggtg
     7921 gttccaagac cgttcaattt agtctgaact gctgttgacc aaactcaaca gccgctcgcg
     7981 ctccgcgccc gcgacgcgca cgagtttcgt cttcgacgag tgtccgctcg tgatctcgac
     8041 ggcgcgcaca ggcacgccga gcgcgcgtgc cagcgtgcgc gcgagttcga cgttagccgc
     8101 gccttcgacg ggcggggcgg ccacgcgcac gcgcagcgcg ccgtcgtgct cgcccacgac
     8161 ctcgctctgc gaagcgcgcg gcacgacacg cacggtgaac acgagcgcgc cgccagtctc
     8221 cgtgcatcgc aacatgaaat ccttccccac ctctccataa atgttactcg accgcgtcta
     8281 caatctaacg cgcttttgag agtgttatag tgtagccgca atataatcaa ccggaaggag
     8341 gatttatgac taactacgag atagtgccgg ctcgtattcc tgcgaatgcg cgcgaggtgg
     8401 agatatttga ggtctggcta gaaagactct ccgcggctgg agccgaattg gttaatacga
     8461 tgcccagcgt gacaagcaat acaatcctat gcatcttccg cgttcagagt aaaggaactg
     8521 tcgctgcgag taatgttccg taactggcaa aggggtgaca gggagatttc gacttttaac
     8581 cgtgaccttc aaattggctg gaggggcgac attatgctca cccctgtagc cgattaaaag
     8641 cgcatgagcg ttccggcaat ggccgtctgg aagagccaga tgagcaggaa ggcgacgagc
     8701 ggtgagatgt cgaagaagcc gagcggtggg atgatgcgcc gcagcggcac gagcagcgga
     8761 tcggtggccg tcaccagaaa tcgcatcacc ggattacgca agccgaccat gccccacgag
     8821 aagatgatgc gaatcaaaat gagcatcgaa tagacgctca ggaaaccgta gagcagcgtg
     8881 ccgacgagcg cgaccatcct tccttcgcgc gaacgcagaa acattccgta aaacgtcgag
     8941 atgacatcgt tcgacaaact caaggccagc cagccgacga ggatgacgat caggatcgtg
     9001 acgagcggag ccatgttcgg cgtgaagccg aaattcagca gcgaccgccg cacgggattc
     9061 acgaacgggt cggtcagatt gcgcacaaac agcaccggg
//
LOCUS       DASWNS010000207        13320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_477009, whole genome shotgun sequence.
ACCESSION   DASWNS010000207 DASWNS010000000
VERSION     DASWNS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477009"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2314
                     /locus_tag="VGB76_06960"
     CDS             <1..2314
                     /locus_tag="VGB76_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0098679.1"
                     /translation="AEKLSISTRSMLPSGAGDAQRQAGLQSVDPELALAINTANTLAD
                     DSNINAVVVYHQLPTEADLSDLQRIGILGGTRFHVVPALYVSATADQLIAVSRLRNVR
                     SLYKNRTLTLNADPGQGLTGVERVRRDADIMRNNAGTPLSGRGVTVAVLDTGLDSTHS
                     DLAGRVAQNVKLADLQSAPVGFNYPANVENLPNTDQAHGHGTFVAGVIGGNGAKSGGQ
                     YKGVAPGARLVGMSAGDLNLVFVLNGLDYLLERGATLGVRVVNCSFSASTVFDFNDPV
                     NIATKMLTDRGINVVFSAGNTGSGWHTLNPYAVAPWVVSVGATDFRGRLADFSSRGDF
                     ASQMFRPTLVAPGVRVVSLRSSGLSVTGVEGIGFNGDAGLAPGNVPFYTIASGTSFSA
                     PQVAGAIALMLEANPKLTPAQVRDILQRTATPLASYYAHEVGAGMLNTHAAVLEAVFP
                     ERKLGSFRATLNRGQVRFLNDPLREISGAVAPGAMGFEASVEMPAGALVASMQIGWSM
                     LSVNDLGLAMYDASGAKQADENEINLPGLAGNRERAVINAPAAGVWNVKVRNTLGGLA
                     VTPQKFAGVLEVARAEYAPLKDLDGLSASQRNVVYQNIRSFMMSPTGNKFRPLQGVTR
                     GELAEALVLGARVPQYVPAAASYRDVRSSASMLFIESVQHAPQGALFTDVERGGAFSP
                     NEAAGRLTAAVALVRAAGLQAEAEAVQNMPLTTLLDAGLIPAALRGYVRVAISHGLLS
                     TSNAYFRPQQPLTRLELAHAMIALQKMQAQ"
     gene            complement(2456..4240)
                     /locus_tag="VGB76_06965"
     CDS             complement(2456..4240)
                     /locus_tag="VGB76_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0098680.1"
                     /translation="MKLVQLGRSLIASLLISALLCVSACVTSPPATNNANVNQRQALT
                     EKVVGTRGGSLTYRITSPPKTFNYILAADEPSNFVAFFLTGSRLVELDHDTQNYVPAL
                     AESWKLSDDELSVEVVLRDGLKFSDGHALTSEDVAFTLRALYDERVLSPIYSDAMHVG
                     GKPITITVADPRRFRLGFAEAIAVPENYMSNLNVLPRHVLEPVLEKNGFNQAYSVTSD
                     VAGVVTAGAFAVESSTPGERVALKRNPHYWKRDAQGNPLPYLDRLVLEVVTDENNAVA
                     RLGQGTLDILDRVRPTDHATLRSGQGAAARSFDLGPGLYADNFWFNQNPGQREDGKPI
                     VDPAKLAWFTDVRFRRAVSHAIDRDSIATNTWQGLATPLYNFVSSGNRAWVAADVPRT
                     EYDLEKARTLLREAGFSFRGTESAPELFDAKGKRVEFTIIAPAGNEVRVKSAIVIQED
                     LKKLGMNVQVAPIDNARLSALIGTSYDYEAVLFGTSATEPDPSSYTDMLHSGSVQHLW
                     HPKQPKPATEWEARLDELNTRQAHEVDRERRRAIFRDIQLLMAEQMPLIPIVTRHIAV
                     AANPRIRNYRPSTLPPFSLWNAEELFVR"
     gene            4458..5465
                     /locus_tag="VGB76_06970"
     CDS             4458..5465
                     /locus_tag="VGB76_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098681.1"
                     /translation="MLKLILSRSLQGIVVLLVVSALTFALLAAAGGDALGDLSMEQTA
                     SDASIRELRRVYGLDEPLVVRYVRWLGGLVRGQMGDSFYYHAPVATIIWPRLLRTLAL
                     AAVALVLALSVSLTLGALAARRKGSWWDRASEIIILLTASTPRIVLALMALAIVARTS
                     WFPVGGADTGESLMDFRPLRILPPAIVLCLPLVALFLAQTREGLSHALGQDFVRVARA
                     KGLSERAVIMRHALRDALNPLITLFGYSFGSVVSGSVIVETVLGWSGLGSLSVRAVLT
                     RDVPLLMGVVMITATAVLVGNLLADIMLRLNDPRLRAHGPAGNPRRGAGDALNLPASG
                     A"
     gene            5474..6715
                     /locus_tag="VGB76_06975"
     CDS             5474..6715
                     /locus_tag="VGB76_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098682.1"
                     /translation="MSNTQPPHVSDAHARAESHALSPTSPPQDDWERAHAAATTWRGR
                     ARRRFARRWKLIVGLSIVSTFYVVAACANFIAPYDASEQSKREPLAPPTALRFTDAEG
                     RWHARPFIYKLRLDDPLARTYAEDTSARYPLALFTRGSSYKLFWLFNTDRHLFGVAPD
                     AQRSAAQPPGASARVANADVQRVYLLGTDEFGRDRLSRLLLASRFSLAVGPLGTLFAG
                     VLGVLLGCISGYAGRRTDALLMRAADAMLALPELVLILAARAAFPAELPPMHAGLLLV
                     SIFVAIGWAEMARLARGLVMGLREREFVLAAVSLGLSETRILFRHILPNAARPLIVQL
                     SLMLPAFLLAETALSFLGVGVQEPAASWGNMLKSASELSLLARQPFLLLSPAFAIFLF
                     VLGVRLLSDGLKRDDGDLRAK"
     gene            7058..9577
                     /locus_tag="VGB76_06980"
     CDS             7058..9577
                     /locus_tag="VGB76_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0098683.1"
                     /translation="MRKLTTILITFIICLALNATAAQAARVSPALSTQLASLADSVKV
                     GTVIVAFNTNEGIKDSHLSILRGLGITRAITFPRLGMAALPTATAGQVRALAGNPDVR
                     SVWDNERIHYYDLQGRTLTGVERVRGDAAFRQRNGNMSVSGKGDFSVLVIDSGVDATH
                     DDLKLGQNVIQNVQVLTGTDTVQEGYTPVTVLENVPNTDQTVGHGTHCAGIIGGTGQR
                     SGGKYAGVAPGTKIIGAGLGAGLFVINALGAWEWGLANQYNYNIKVVSNSYGSFAPFD
                     PDNPINIASKAAYDNGITVVFAGANSGPGKNTYNKYAKAPWVIGVAAGTKEGGLASFS
                     SRGTPADERFSNSDPLDDYDAPTITAPGTGREFESNAAKFSAAYVSTRSTSNITANGL
                     DADAELAPGEIPFYTQISGTSMAAPYVAGVVALMLDADPGLTPDEIRRIIIETATRMP
                     GYADHEVGAGYINAYAAVDKVFNRTRVYNAFNQANFNAKYSITIPVALPFHIDFNPAA
                     LPGADSPNAKRFTVQAGMSVLDAYARVDTAAATGDGNLVGILLTAPDGTTYSSGVQYP
                     VIGASSRGVIVKNPLPGEWVLEVRGARGLVAVPAASLPTSGAAAPGAVDGIILQKLFT
                     LAPVADIQDHEQQALFETLLKSRRMDTLADGLFHPEAAVTREDFARTLGLNAPVRQTA
                     FASHRFTDVSGELAALVDALTVNGSTLRDYNFAPAGLLSASGTNFNPNGNVSRLDLAV
                     AFVRALGLDAEAKAKAGTNVTSGGITLTDNAQIPASLRGYVQFAIDRGFLEVYPAEVK
                     QVAPGQFIALPGPRVEPANGLTRAALAAKVNTFAERFAAGN"
     gene            9724..13275
                     /locus_tag="VGB76_06985"
     CDS             9724..13275
                     /locus_tag="VGB76_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY0098684.1"
                     /translation="MSSPTAPVNRKARPRTGRIFFALFALVLVTVVVGATFISPARTS
                     ASSASVSDKLDLNKTPVPDYNVNLGKSVVRLPSASQLQGLNTLKANLNDAQITARWDK
                     TTGSVDTIMDFASQPSALEPEAAAREFIAANAAIFGISDMTSLRLKRNTPALGGNLLY
                     FEQMYQGMPVAGRGVGVVLDGEGRVKSVSGPYQKDLNIALNPSLDGAAAVVKAQSDLA
                     KFKVQWVQGVADVLNPALDKLASQLGPMAVPHPQLNIFPTTAGPRLAYKFLVFSRNPF
                     GAFKYQIDAATGEVLYREDIVRYQQQLEPKADVFPTYPCISKKLQDEGIIENGANGAP
                     CGQLRVNLRKFDPTNIVTGVNGTLTGTHAHIENVLAGKLPFAQAALGTWYFAKDDPTN
                     FEARTTDAAHFGPAAEPAEHQGEISQFFYITSLLEYIDYLHVAGDLKHSRVGQGSFPD
                     TYPNQASPLIGNVHIPNVLAPPTDPSDPEFAAKLLGLDNAFSVSASSEEFLGATPGGQ
                     NVVVNPTSYGHGYLLNDLAIDFAVPYHEGMHSISSPIAGLENDPNGAPEGSALNEAQA
                     DLWAYTAGENPVLGNYILNAKDYRAAVRANGGNPDERQYLRHGDSGLLYSQLGTQGGS
                     AFEEHRDGEIFAAAGWDLRELMILAEQGGSFARPDLITGEATKSISRGQENWERILLG
                     AIYVLSTYNPDTMVRGRDAMIIADQSLYPSDPSDPDAPGQHRALIEQVFAARELGVNA
                     VAPNAEGRQAISTRVSDFAASVNRLSAPSGVSVVPASSKANQISWQPVAGAFAYEVLR
                     REIGKENVRQTKPVTGREYMDGDGATDGFMHIAYVKGNESGYTDSGLIEGVFVPRGLK
                     NPASYEYVVRALNVNSKRQVGVSDNSAVAAMSTAVVDVTNSIQTANSNVSFAGGKTEF
                     DQTIKNLGAGAFDGTIYQPIDFRIVSISAPSVSVANADNFGNGRGASPASFYYRSTLT
                     KNQTSAARRIVFNNPNTQLFTFDAVVTARVQVPAGAGTRFEAEPKFDGSFTTSSFSES
                     FTGIVPASDTGLQLADGVTYVDVPFTSQSNAVAVKGILTSPLTGIDLDFELRDAAGRV
                     LSSSGTSSAQEIVTAEILPNANYVFRVIGWAGAAQDFEIASTQTLRIPQNAGGTTSGT
                     TGTSGGSTLTGGLTTATRLVRFTVNPLTRSITMQVLR"
BASE COUNT         2437 a         4468 c         4025 g         2390 t
ORIGIN      
        1 cgccgaaaag ctctcgatct cgacgcggag catgctccct tcgggagcgg gcgatgcgca
       61 gcggcaagcc ggactgcaaa gcgtcgatcc cgaactcgcg ctcgccatca acactgcgaa
      121 cacgctcgcg gacgacagca acatcaacgc cgtcgtcgtc tatcaccaac tcccgaccga
      181 ggccgatctc tcggacttgc agcgcatcgg cattctcggc ggcacgcgtt tccacgtcgt
      241 cccggccctc tacgtctcgg ccaccgccga tcaactcatc gccgtctcac gcctcagaaa
      301 cgtgcgctcg ctttacaaga accgcacgct gacgctgaac gccgatccgg gacagggctt
      361 gacgggcgtt gaacgcgtgc gccgcgatgc ggacatcatg cgcaacaacg cgggcacgcc
      421 tctcagcggg cgcggcgtga cggtcgccgt gctcgacacg ggtttggatt cgacgcacag
      481 cgatctggca gggcgcgtcg cacaaaacgt caaactcgca gacctccaga gcgcgcccgt
      541 cggtttcaac tatcccgcca acgtcgaaaa tctcccgaac acggatcagg cacatggaca
      601 cggcacgttc gtcgcgggcg tgatcggtgg taacggtgcc aagtcgggcg ggcagtataa
      661 gggcgtcgcg ccgggcgcgc ggctcgtcgg catgagcgcg ggcgatctca atctggtctt
      721 cgtcttaaac ggcctcgatt atctgttgga gcgcggggca acgctcggcg tgcgcgtcgt
      781 caattgcagt ttctccgcga gcaccgtctt cgacttcaac gatccggtca acatcgcgac
      841 gaagatgctc acggacagag gcatcaacgt cgtcttctcg gcgggcaaca cgggttccgg
      901 ctggcacaca ctgaatcctt acgcggttgc gccgtgggtt gtctccgtcg gcgcgaccga
      961 ctttcgcgga cgcctcgcag acttctcttc gcgcggcgac ttcgcgagcc agatgtttcg
     1021 ccccacgctc gtcgcgcccg gcgtgcgcgt cgtgagcctg cgttcgagcg ggttgagcgt
     1081 gacgggcgtg gagggcatcg ggttcaacgg cgacgcgggg cttgcgccgg gcaacgtgcc
     1141 tttctacacg atagccagcg gcacgagttt ctccgcgccg caggtggccg gggcgattgc
     1201 gctcatgctc gaagcgaacc cgaaattgac gcccgcccag gtgcgcgaca tcctgcaaag
     1261 aaccgccacg ccgctcgcct cttactacgc gcacgaagtc ggcgcgggga tgctcaacac
     1321 gcacgcggcg gtgctcgaag ccgtgttccc cgaacgcaag ctcggcagtt ttcgtgcgac
     1381 tctcaatcgc gggcaggtac gcttcctgaa cgatcctctg cgggagattt ccggcgcggt
     1441 cgcaccgggc gcgatgggtt ttgaggcgag cgtggagatg cctgcgggcg cgctcgtggc
     1501 ctcgatgcag atcggttgga gcatgctcag cgtcaacgat ctcgggctgg cgatgtacga
     1561 tgcgagcggc gcgaagcagg ctgacgagaa cgagatcaac ctgccgggct tggcaggcaa
     1621 tcgcgagcgc gcggtgatca atgcgcctgc ggctggcgtc tggaacgtga aggtcaggaa
     1681 cacgctcgga gggttggcgg tcacgccgca gaagttcgcc ggagtgcttg aggtggcgcg
     1741 tgcggaatac gcgccgctca aagacctcga cgggctgagc gcgtcgcagc gcaatgtcgt
     1801 ctatcaaaac atacgctcgt tcatgatgtc gccgacgggg aataaattcc gcccgttgca
     1861 gggcgtcacg cgcggcgaac tggcggaagc gttggtgctc ggcgcgcgtg tgccgcaata
     1921 cgtgcccgcc gccgccagtt atcgagacgt gcggagttcg gcgtcgatgc tctttatcga
     1981 gagcgtgcag catgcgccgc agggcgcgct ctttacggac gtggagcgcg gcggggcgtt
     2041 ctcgccgaat gaagcggcgg ggcgtcttac ggcagcggtc gcgcttgtcc gcgcggcggg
     2101 actgcaagcc gaagccgagg ccgttcaaaa catgccgctc accacgctcc tcgacgcggg
     2161 gttgatcccc gccgccctgc gcggctacgt ccgcgtcgcc atctcgcacg ggctgctctc
     2221 aaccagcaac gcatacttcc gcccgcagca accgctcaca cgtctcgaac tcgcgcacgc
     2281 catgatcgcg ttgcagaaaa tgcaggcgca gtaaggaacg gtgaaagggt aaggggtcaa
     2341 gggaacaggg tggagacaaa agcgggacgc agcgtttaag tgctgcgtcc cgcttcctgt
     2401 ttttaccctc gcctcttgcc ccttaccctt gccccttacc tttcctcttt ctgcttcatc
     2461 tcacaaacaa ctcttccgcg ttccacagag agaagggcgg gagcgtgctg gggcgataat
     2521 tgcgtatgcg cgggttggcg gcgacggcga tgtggcgcgt gacgatggga atgagcggca
     2581 tctgttcggc catcaggagt tgaatgtcgc gaaagatggc gcggcgtcgt tcgcggtcaa
     2641 cttcatgcgc ctgccgcgtg ttcagttcgt cgaggcgcgc ttcccattcg gtggcgggct
     2701 tcggctgttt cggatgccag agatgctgga cgctgccgct gtgcagcatg tccgtatagg
     2761 aagagggatc aggctcggtg gccgacgtgc cgaagagcac cgcttcgtaa tcataagagg
     2821 tgccgatgag cgcggacaat cgcgcgttat cgatgggcgc aacctgcacg ttcatcccta
     2881 acttcttcaa atcttcctga atgacaatcg cgctcttcac acgcacttca ttaccggcgg
     2941 gcgcgatgat cgtaaactcc acgcgcttgc cttttgcatc gaacagctcc ggcgcactct
     3001 ccgtgccgcg aaaactgaag cccgcctcgc gcaacagcgt gcgcgctttt tccagatcgt
     3061 actcggtgcg cggcacgtcc gcagcgaccc acgcgcggtt gccgcttgag acgaagttgt
     3121 agaggggcgt ggcgagtccc tgccaggtgt tcgtggcgat tgagtcgcgg tcgatggcgt
     3181 gcgagacggc gcgacgaaaa cgcacgtccg taaaccaggc gagtttcgcc gggtcaacaa
     3241 tcggcttgcc gtcttcgcgc tggccgggat tctggttgaa ccagaaattg tctgcgtaga
     3301 ggccgggtcc gaggtcgaaa gagcgcgccg ccgcgccttg cccggagcgc agtgtggcat
     3361 ggtcggtcgg gcgcacgcgg tccagtatgt cgagcgtgcc ttgaccgagc cgcgcgaccg
     3421 cgttgttttc gtctgtcacc acttcgagca cgagacggtc gagataagga agcggattgc
     3481 cctgcgcgtc gcgtttccaa tagtgcgggt tgcgctttaa ggcgacgcgc tcgcccggcg
     3541 tcgaggattc aacggcgaag gctccggcgg tgacaactcc ggccacgtcg ctcgttacgc
     3601 tgtatgcctg attgaagccg ttcttctcca gcacgggttc gagaacgtgg cgcggcagca
     3661 cgttcaaatt cgacatgtag ttttccggca cggcgatggc ttcggcgaag ccgagacgaa
     3721 agcggcgcgg gtctgccacc gtgatcgtga tgggcttgcc gccgacgtgc atcgcatcgc
     3781 tatagatggg cgagagcacg cgttcgtcgt agagcgcacg gagcgtgaag gccacatctt
     3841 cactggtcag cgcgtggccg tcggagaatt tcaacccgtc gcgcaagacg acttcgacgc
     3901 tcaactcgtc gtccgagagc ttccaacttt cggcgagggc gggcacgtag ttttgcgtgt
     3961 cgtggtctaa ctccacgagg cggctgcccg ttaaaaagaa ggcgacgaaa ttagagggtt
     4021 cgtcggcggc gagaatgtag ttgaaagttt tcggcggcga cgtgatgcga taggtgagcg
     4081 agccgccgcg cgtgcccacg actttttcgg tcaacgcctg tctttgattg acgtttgcgt
     4141 tattcgtcgc gggcggcgaa gttacacacg cgctgacaca gaggagcgcg gagataagta
     4201 aggaggcgat gagagatcgt ccgagctgca caagtttcat ttcaaaatac cgggtgtctg
     4261 aagatattaa aaagcgcaag gttgtttgtg cgaactacgc gcacgagtga ggttgtttga
     4321 ttcaaacatg tctgccagcg gatgtccaga atttatctgc gccgaactct tgcgcgctcg
     4381 ccgcgaatag aggttaaatc aaccagacga tttagaaggt gaactgacac tctcgccgat
     4441 ttgttcaggc tgtaaacgtg ctgaaactga ttctctccag atcgctgcaa ggcatcgtcg
     4501 tgctgctcgt cgtctcggcg ttgacctttg cgctgctggc cgccgccggc ggcgatgcgc
     4561 tcggcgacct cagcatggag cagacggctt cggatgcgtc gatacgcgag ttgcgccgcg
     4621 tctatggact cgacgagccg ctcgtcgtgc gttacgtgcg ctggctcggc ggtctggtgc
     4681 gcgggcagat gggcgactct ttctactacc atgcgcccgt ggcgacgatc atctggccgc
     4741 gcctgctacg cacgctcgcg ctcgccgccg tcgcgctcgt gctcgcgctc tccgtctcgc
     4801 tcacgctcgg cgcgctcgcc gcacgccgca aaggctcctg gtgggacagg gcgagcgaga
     4861 tcatcatcct gctcacggct tcgacgccgc gcatcgtgct cgcgctcatg gctctcgcca
     4921 tcgtcgcgcg cacttcatgg tttcccgtcg gcggcgcaga cacgggggag tcgctgatgg
     4981 actttcgccc cctgcgcatc ctgccgcccg ccatcgtcct ctgccttcca ctcgtcgcgt
     5041 tgtttctggc gcagacgcgc gaaggtttga gccacgcgct cggacaggac ttcgtgcgcg
     5101 tggcgcgtgc gaaaggattg tccgaacgcg ccgtgatcat gcgccacgcg ctgcgcgacg
     5161 cgctgaaccc gctcatcacg ctcttcggtt attcgtttgg cagcgtcgtc agcggctcgg
     5221 tcatcgtgga gacggtgctc ggctggtctg gcctcggctc gctcagcgtc agggcggtgc
     5281 tcacgcgcga cgtgccgctc ttgatgggcg tcgtgatgat cacggcgacg gcagtgctcg
     5341 tcggcaacct cctggcggac atcatgctcc gtctcaacga tccgcgccta cgcgcacatg
     5401 gtcccgcagg caacccccgg cgcggcgcgg gcgacgcgct caacctgccc gcttccggcg
     5461 cgtgaatcaa cgcatgagca acacgcaacc gcctcacgtc tccgacgcgc acgcacgcgc
     5521 cgagtcgcat gcactgtcgc caacgtcgcc gccgcaggat gactgggagc gcgcgcatgc
     5581 ggcagcgacg acgtggcgcg ggcgtgcgcg tcgccgtttc gcgcgccgct ggaagttgat
     5641 cgtgggactg agcatcgtct cgacgtttta cgtcgtcgcc gcgtgcgcga atttcatcgc
     5701 gccttatgac gcgagcgagc agtcgaagcg cgaaccgctc gcgccgccca ccgccctgcg
     5761 cttcacagac gccgaaggcc gctggcacgc gcgccctttc atctacaaac tgcgcctcga
     5821 cgatccgctc gcgcgcacct acgccgaaga cacctccgcg cgttacccgc tcgcgctctt
     5881 cacgcgcggc agttcttaca aactcttctg gctgttcaac accgaccggc atctcttcgg
     5941 agttgcaccc gacgcgcagc ggagtgccgc acaaccgccg ggcgcaagcg cgcgagtcgc
     6001 caacgcagac gtgcagcgcg tctacctgtt aggcacggat gaattcggac gcgaccgtct
     6061 ctcgcgcctg ctgctggcgt cccgtttctc gctcgccgtg gggcctctcg gaacgctgtt
     6121 cgcaggcgtg ctcggcgtgc tgttgggctg catctccggc tacgccgggc gcaggacgga
     6181 cgcgctcctg atgcgcgcag ccgacgccat gctcgctttg cccgaactcg tcctgatact
     6241 ggcggcgcgt gcggccttcc ctgccgaact gccgccaatg cacgcggggc tgctgctcgt
     6301 ctcgatcttc gtcgcaatcg gttgggcgga aatggcgcgg ctggcgcgcg ggttggtgat
     6361 gggtttgcgc gagcgcgagt ttgtgctggc cgccgtgtcg ctcggactga gcgagacgcg
     6421 catcctgttt cgacacatcc tgcccaacgc cgcgcgcccg ctcatcgtgc aactctcgct
     6481 gatgctgccc gcgtttctgt tggccgaaac cgcgctttcg tttctcggcg tcggcgtgca
     6541 ggaaccggcg gcgagttggg gcaacatgct caagtctgcg agcgaattgt ctctgctcgc
     6601 gcgccagcct ttcctgttgc tctcgcccgc cttcgccatc ttcctcttcg tactcggcgt
     6661 gcggctactc agcgacggac tcaagcgcga cgacggcgac cttcgcgcaa aatgagacgc
     6721 cgccacgccg caacgcattt gcaataaaaa cttaactcgc ccacaatcaa gctcgacttc
     6781 ccgacttaaa ctctcccctt gcggctcagt tttttctcca caccgcccgc catcctgtct
     6841 cagccagcga cgcgagcgac gttttggtct gcaccttcac cgccggacat acacgccttg
     6901 caacgcgttt gcaataatct caagcgcatc gctttccgcc tcaagtctgc ctcaagtttt
     6961 cctcaatagc ctccgcttac tttcacgcca gaagagcaga cgacatgctt tcttcaaatt
     7021 aaaaattccg acagggttca acggaggcgc ttctttaatg cggaaactta cgaccatcct
     7081 gatcacgttc atcatctgcc tcgctctcaa tgccacagcg gcacaggcgg ctcgcgtcag
     7141 ccccgcgctc tctacgcaac tcgcttcgct cgccgattcg gtcaaagtcg gcacggtcat
     7201 cgtcgccttt aacacgaacg aagggatcaa ggattcgcac ctctcgatcc tgcgcgggct
     7261 gggcatcacg cgcgccatca cttttccgcg cctcggcatg gccgcgctcc cgacggccac
     7321 cgccggacag gtgcgcgccc tcgccggaaa cccggacgtg cgctccgtct gggacaacga
     7381 gcggattcac tattacgact tacaggggcg cacgctcacg ggcgtcgagc gcgtgcgcgg
     7441 cgacgccgct ttccgccagc gcaacggcaa catgtcagtc tccggcaaag gcgacttctc
     7501 cgtcctcgtc atcgactccg gcgttgacgc cacccacgac gatctcaaac tcggccagaa
     7561 cgtcatccag aacgtgcagg tcttaacggg caccgacacg gtgcaggaag gctacacgcc
     7621 cgtcaccgtc cttgagaacg tgcccaacac ggaccagacg gtcggccacg gcacgcactg
     7681 cgcgggcatc atcggcggca caggccaacg ctccggcggc aagtatgcgg gcgtcgctcc
     7741 gggcacgaag atcatcggcg cgggactcgg cgcggggctg ttcgttatca acgctctcgg
     7801 cgcgtgggag tggggactgg cgaaccagta caactacaac atcaaagtgg tatcgaactc
     7861 ttacggcagc ttcgctcctt tcgaccccga caacccgatc aacattgcga gcaaggcagc
     7921 ttatgacaac ggcatcaccg tcgtcttcgc gggcgcaaac tctggtcccg gcaagaacac
     7981 ttacaacaag tatgcgaaag cgccctgggt catcggcgtg gcggcgggca cgaaagaggg
     8041 cggcctcgcc tctttctcct cgcgcggcac gccagccgac gaacgcttct cgaactccga
     8101 cccgctcgac gattacgacg cgccgaccat taccgcgccc ggcacgggtc gcgaattcga
     8161 atcgaacgcc gcgaagtttt ccgccgctta cgtctcgacg cgctccacct cgaacatcac
     8221 ggccaacggc ctcgacgccg acgccgaact cgcccccggc gagattccct tctacacgca
     8281 gatcagcggc acttcgatgg ccgcgcccta cgtggccgga gtcgtcgccc tgatgctcga
     8341 cgcagacccc ggtttaacgc ccgacgagat caggcggatc atcatcgaga cggcgacgcg
     8401 gatgcccggc tacgcggacc acgaagtcgg cgcgggctac atcaacgcct acgccgccgt
     8461 ggataaagtc ttcaaccgca cgcgcgtcta caacgctttc aaccaggcga acttcaacgc
     8521 caaatacagc atcaccatcc ccgtagccct gccgtttcac atcgacttca acccggcggc
     8581 cttgcccgga gcggattcgc cgaacgcgaa gaggttcacc gtgcaagcag gcatgagcgt
     8641 gctcgacgcg tacgcgcgcg tcgatacggc ggcggcgacg ggcgacggca acctcgtcgg
     8701 catcctcctg accgcacccg acggcacgac ctattcgagc ggcgtccagt atccggtgat
     8761 cggtgccagc agtcgcggcg tcatcgtcaa aaatcctctg cccggcgagt gggtgctgga
     8821 agtgcgcggc gcgcgcggcc tcgtcgccgt ccccgccgcg agcctcccca cttccggcgc
     8881 agccgcgccc ggggcggttg acggcatcat cttgcagaag cttttcacgc tcgcgcccgt
     8941 cgcggacatt caagaccacg agcagcaggc tctcttcgag acgctgctca agagccgccg
     9001 catggacacg ctcgcagacg gactcttcca ccccgaagct gccgtgacgc gcgaagactt
     9061 cgcacgcacc ctcggcttga acgcgcccgt gcgccagacg gcttttgcat cgcacaggtt
     9121 tacggacgtg tccggcgaac tcgccgcgct cgtcgatgcc ctgaccgtga acggttcgac
     9181 gcttcgcgac tacaacttcg cgcccgccgg actcttgagc gcaagcggca caaactttaa
     9241 cccgaacggc aacgtcagcc gtctcgacct cgccgtcgcc ttcgtgcgcg ccctcggcct
     9301 cgatgcggaa gcgaaagcga aggcgggcac caacgtcacc tccggcggca tcacgctgac
     9361 ggacaacgcg cagattcccg cgagccttcg cggctacgtg cagttcgcca tagatcgcgg
     9421 cttcctcgaa gtctatccgg cggaagtcaa gcaggtcgcg cccggccagt tcatcgcgct
     9481 gccggggccg cgcgtcgaac ctgcgaacgg cctcacacgc gccgcgctcg ccgcgaaagt
     9541 caacaccttc gcggagcgtt tcgcggctgg caactaattc gataaacgcc ctcgtgacgc
     9601 cgccgcaaca gacggcgtca cgggcgcgat gacacaaagc atccttcgca caaaccggca
     9661 gcaattttta gagattcagc gaatctcagg cgagagccgt aaaaatttta caggagaaaa
     9721 acgatgagtt caccgaccgc acccgttaat agaaaagctc gcccccggac gggcagaatc
     9781 ttcttcgcac ttttcgcact cgtcttggtc accgtcgtcg tcggcgcgac gttcatcagc
     9841 cccgcacgca cgagcgcgag ttcggcctcc gtctccgaca agcttgactt gaacaagacg
     9901 cccgtgcccg actacaacgt caacctcggc aaaagcgtcg tgcgcctccc ttccgcgtcg
     9961 cagcttcagg gacttaacac gctgaaggcc aacctcaacg atgcgcagat caccgcgcgc
    10021 tgggacaaga cgaccggctc ggtggacacc atcatggatt tcgcctcgca gccctccgcg
    10081 ctcgaacccg aagcagcggc gcgcgaattc atcgcggcca acgccgccat cttcggtatc
    10141 agcgacatga ccagcttgcg cctcaagcgc aacacccccg cgctcggcgg caaccttctc
    10201 tattttgaac agatgtatca gggcatgccg gtcgcgggtc gcggcgtcgg cgtcgtgttg
    10261 gacggcgaag ggcgcgtcaa gtccgtctcc ggtccttatc aaaaagattt gaacatcgca
    10321 ttgaacccgt cgctcgacgg cgcggcggcg gtcgtcaaag cgcagagcga tctggcgaag
    10381 ttcaaagttc aatgggtgca gggcgtggcc gatgtcctga atcccgcgct cgacaaactc
    10441 gcttcgcagc ttggtccgat ggccgtgccg cacccgcaac tcaacatctt cccgacgact
    10501 gcggggcctc gtctcgctta caagttcctc gtcttctcgc gcaacccgtt cggcgcgttc
    10561 aagtatcaga tcgacgccgc gaccggcgag gtgctctacc gcgaagacat cgtgcgttat
    10621 caacagcagc ttgagccgaa ggcagacgtg ttcccgacct atccctgcat cagcaaaaag
    10681 ttgcaggacg aaggcatcat cgagaacggc gcgaacggcg caccctgcgg ccaactccgc
    10741 gtcaacctcc gcaagttcga cccgaccaac atcgtgaccg gcgtgaacgg cacgctcacc
    10801 ggcacacacg cgcacatcga aaacgtgctc gccggaaaac tcccgttcgc gcaggccgcg
    10861 ctcggcacct ggtacttcgc caaagacgac ccgaccaact tcgaggcgcg caccaccgac
    10921 gccgcccact ttggacccgc tgccgaaccc gccgagcatc agggcgaaat ctcgcagttc
    10981 ttctacatca catcgctgct ggaatacatt gactacctgc acgtcgcggg cgatctcaaa
    11041 cacagccgcg tcgggcaggg cagtttcccc gacacttatc cgaatcaggc aagccccttg
    11101 atcggcaacg tccacatccc gaacgtgctc gcgccgccga ccgatccgag cgacccggaa
    11161 ttcgcggcca agctgctcgg cttagacaac gccttctccg tctccgcttc gtccgaagag
    11221 tttttgggcg cgacgcccgg cgggcagaac gtcgtcgtca acccgacctc ttacggccac
    11281 ggttacctct tgaacgatct ggcgattgac ttcgcagtgc cctaccacga aggcatgcac
    11341 tcgatctcca gccccatcgc cggactcgaa aacgatccga acggtgcgcc cgaaggcagc
    11401 gcgctcaacg aagcgcaggc cgacctgtgg gcttacacgg cgggcgaaaa ccccgtcctc
    11461 ggcaactaca tcctgaacgc caaagactat cgcgcggcgg tgcgcgccaa cggcggcaac
    11521 cccgacgagc gacagtatct acgccacggc gactccggcc tgctctattc gcaactcggc
    11581 acgcagggcg gcagcgcgtt cgaggagcat cgcgacggcg agatcttcgc cgccgccggt
    11641 tgggacttgc gcgaactgat gattctggcc gaacagggcg gcagcttcgc gcgccccgat
    11701 ctcatcacgg gcgaagcgac gaaatcgatc agtcgcggtc aggagaactg ggaacgcatc
    11761 ctgctcggcg cgatctacgt gctctccacc tataacccgg acacgatggt gcgcgggcgc
    11821 gacgccatga tcatcgccga ccagtcgctc tacccttcag acccttcaga cccggatgcg
    11881 cccggccagc accgcgctct catcgagcag gtcttcgccg cgcgcgaact cggcgtgaac
    11941 gccgtcgcgc ccaacgcgga aggccgtcag gccatctcaa cccgcgtctc cgacttcgcg
    12001 gcttcggtca acagactgag cgcgccgtcg ggcgtcagcg tcgtcccggc ctcgtcgaaa
    12061 gcgaatcaaa tttcgtggca gccggtggcg ggcgcgttcg cctacgaagt gctgcgccgc
    12121 gaaatcggga aagagaacgt gaggcagacc aaacccgtga cgggtcgcga atacatggac
    12181 ggcgacggcg caacggatgg cttcatgcac atcgcctacg tgaagggcaa cgagtcgggc
    12241 tacaccgaca gcggcctcat cgaaggcgtc ttcgtgccgc gcggtttgaa gaaccccgcg
    12301 agctacgaat acgtcgtgcg cgctctgaac gtgaactcga aacggcaggt cggcgtctcc
    12361 gacaatagcg cggtggcggc gatgtcaact gccgtggttg acgtgacgaa cagcatccag
    12421 accgccaaca gcaacgtctc gttcgcgggc ggcaagaccg aattcgacca gacgatcaag
    12481 aacctcggcg cgggcgcgtt cgacggcacg atctaccagc cgattgattt ccgcatcgtc
    12541 tcgatctccg cgccgtccgt ctccgtcgcc aacgcggaca acttcggcaa cgggcgcggc
    12601 gcgtctccag ccagcttcta ctaccgctcg acgctcacga agaatcaaac ttcggcggcg
    12661 cggcgcatcg tcttcaacaa cccgaacacg caactcttca ccttcgacgc ggtcgttacg
    12721 gcgcgcgtgc aagtgcctgc gggcgcgggc acacgcttcg aagccgagcc gaagttcgac
    12781 ggcagtttca ccacttcaag tttcagtgaa tcgtttaccg gcatcgtccc tgcgagcgac
    12841 accgggctgc aactggcgga cggcgtcacc tacgttgacg tgcccttcac ttcgcagtcg
    12901 aacgcggtcg ccgtcaaagg cattctgact tcgccgctga cgggcattga tctcgacttc
    12961 gagttgcgcg acgctgccgg gcgcgtgctc tccagttccg gcacctcaag cgcgcaggag
    13021 atcgtgacgg cggaaatcct gccgaacgcc aactacgttt tccgcgtcat cggctgggcg
    13081 ggcgcggcgc aggacttcga gatcgccagc acgcagacgc tccgcatccc gcagaacgcg
    13141 ggcggcacga cgagcggcac gacgggcacg agcggcggct cgaccttgac cggcggcctc
    13201 acgaccgcca cgcgcctcgt gcgcttcacc gtcaacccgc tcaccaggag catcacgatg
    13261 caggttcttc gttaagcgac ggcatcgcgc gcaacagaca agcggcgcgt gcgagttcat
//
LOCUS       DASWNS010000208         8035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_242409, whole genome shotgun sequence.
ACCESSION   DASWNS010000208 DASWNS010000000
VERSION     DASWNS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242409"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VGB76_06990"
     CDS             <1..574
                     /locus_tag="VGB76_06990"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0098685.1"
                     /translation="DNGGTASSLSWQRRSSELIADCRVFRVRRDVSVSPHRGSEHDFF
                     VLESSDWINIIPLTANDEVVMIEQYRHGSEEVTLEIPGGMVDAGESPAAAAAREMLEE
                     TGYAATREVVNLGKVRPNPAIHNNWIHTFLARNVELRQKPVIESTEHTVVRLVPLADI
                     PRLISEGAINHALVVVGFHWLLLSAARLAE"
     gene            589..912
                     /locus_tag="VGB76_06995"
     CDS             589..912
                     /locus_tag="VGB76_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098686.1"
                     /translation="MKQGYSVLIIDMSYDEAKNFVMEGFPTVELANEFARRWVRDSVE
                     ELRGLNRTREEVRRLWHTFGEDASVLGGQPHYAGSHELDYFIEHPATATERDWRAIQR
                     LAGIE"
     gene            935..1279
                     /locus_tag="VGB76_07000"
     CDS             935..1279
                     /locus_tag="VGB76_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098687.1"
                     /translation="MRVQLTKLAHARSGDKGDTANVGLIALRAEFYPLLVREVTAARV
                     KEHFEGICKGEVERFELANLGALNFLLHESLGGGGTLSLMTDAQGKTFSTALLRMHIE
                     VPDEEARSLGIS"
     gene            1477..2397
                     /locus_tag="VGB76_07005"
     CDS             1477..2397
                     /locus_tag="VGB76_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0098688.1"
                     /translation="MSREAVSTIPGIRHDYARVGEVRLHYAEAEAGRRSGRLVLLLHG
                     FPECWYSWRHQLTALGTRFHVVAPDLRGYNLSDKPARVEDYRLEKLVGDVLGLIEHFG
                     ARDAAVVGHDWGAAIAWEIAARHPERVRKVAALQVPPLPVWFANMTLKQALSSWYMFF
                     FQLPYLPEWRLGLNDLGGLDGILKKSSRPGTFTDTDIAVYKNALRQKDSRTDTTALTA
                     AINYYRANLFSNVSRFGGGAKGRDARIRVPTLFIYGERDFAIVPETVRDVGKHINAPY
                     RELRLANSNHWVQQESPAEVNAALLDFLES"
     gene            2494..3261
                     /locus_tag="VGB76_07010"
     CDS             2494..3261
                     /locus_tag="VGB76_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0098689.1"
                     /translation="MNEQQAELIYTVEGAVALLTLNRPDKRNALNDALIGELKDALRR
                     ADASEAVRVVVITGAGADFCSGADLSALQKISESSITENLEDAQSLAELFALMRRVRV
                     PVVAAVRGRALAGGCGLATACDLVLATRSARFGYPEVKIGFVPAMVMAILRRNVSEKR
                     AFELITRGAEISAEEAASFGLVNHVFADESFETEVEKYIAGFERVSRSAVMLTKRLLY
                     HMDGMTFDAALQSGADLNAIARMTEDCQQGIARFLKK"
     gene            complement(3283..4173)
                     /locus_tag="VGB76_07015"
     CDS             complement(3283..4173)
                     /locus_tag="VGB76_07015"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEY0098690.1"
                     /translation="MLQEQKSARSIHEQMTTQPAPVPSSPNEGARSLVKLDPEHPGFR
                     DHEYRARRNAIAQIALEYQPGSPVPDAPYTKEEHQLWQIIQDVLRPQHLRHACAEYLE
                     CVERLALPRERIPQLGEVSAKVEALSGFRLEPVGGLVEPRVFLETLASGVFLSTQYIR
                     HHSTPLYTPEPDVVHELLGHAVLLASPPLAELNRLVGAAVKRTRRAEDLERLARVYWF
                     TIEFGVVREAGEVKAYGTGLVSSAGELEALKEAELRPLDLAAAARQEFDPTNYQPVLF
                     CADSFESMYQTLRDYLLRWP"
     gene            complement(4524..4955)
                     /locus_tag="VGB76_07020"
     CDS             complement(4524..4955)
                     /locus_tag="VGB76_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0098691.1"
                     /translation="MAAKLLEFAFFKRRREYGALFIRLIIGSFIIWGVQDNVFSYAQM
                     QEFAHFLEARGVPFPLFAAFLSAYTQFVCGILILLGAFVRLISIPFIVNFIAAIFIAH
                     RGDTIQRTFPALLMVFVGLFFLFHGAGKPSLDEWRERRGAR"
     gene            complement(5208..5843)
                     /locus_tag="VGB76_07025"
     CDS             complement(5208..5843)
                     /locus_tag="VGB76_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0098692.1"
                     /translation="MPDEEPTVETRALVVEARDSHVVIRLNRPAERNALSVATLEELE
                     ALVSTLVVRADISAIIFTGTGDSFASGANIRELRALTPETAKEFALRGQSLMQKIADA
                     PQLTIAAINGYCMGGGLDLALACRLRYAVPDAVFAHPGARLGIITGWGGTQRLPRLIG
                     QTRALEIFTTARRVTAHEAYEIGLVNRLGEPVLECALALAGAACVQARRDG"
     gene            complement(5869..6273)
                     /locus_tag="VGB76_07030"
     CDS             complement(5869..6273)
                     /locus_tag="VGB76_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEY0098693.1"
                     /translation="MSERKIRVLVAKPGLDGHDRGAKIIARALRDAGMEVIYTGLRQT
                     PEMIAAAALQEDVDAVGVSILSGAHNTLCPRIVQLLQERGMDDCLVLVGGIVPREDVP
                     RLKEMGVAEVFLPGTSTEEIVKFLRENVQPRA"
     gene            complement(6387..>8035)
                     /locus_tag="VGB76_07035"
     CDS             complement(6387..>8035)
                     /locus_tag="VGB76_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY0098694.1"
                     /translation="AAVERWEDETLAPVLQKRPERKKRFDTVSLDAVERLYTPADTSG
                     IDFDKDIAFPGEFPYTRGIHPTGYRGKLWTMRQFAGFGTPEETNARFKYLMAQGQTGL
                     SVAYDLPTLMGYDADAMLSEGEVGKCGVAVSSLADMETLFDGIPLEEVTVSQTINAPA
                     SVLLAMYLVVAEKQGADWRKISGTLQNDILKEYIAQKEWIYPIRPAMKLVVDTFEFCT
                     RHVPKYNPISVSGYHIREAGSTAMQELAFTLRDGIEYVQWGVDAGLDVDEFVPRISFF
                     FNAHNDFFEEIAKYRAARRIWSRMMRERFQAKDPRTLQLRFHTQTAGVSLTVQQPLNN
                     IVRVAIQALAGVLGGTQSLHTDAYDEALALPTDKAALIALRTQQIIAEETGVVNTVDP
                     LGGSYFLESLTKKMEDGALDYFAKIDAMGGMVSAVEKGFPQREIQESAYQYQKAVERG
                     EQTIVGVNKYQMDDDDSKIDILVIDESVREHQIARLEQARTRRDDGAVANALEKLKLA
                     AQAGDNTMPATIEAVRAYSTLGEICDALRDVYGLYEEPAF"
BASE COUNT         1607 a         2386 c         2485 g         1557 t
ORIGIN      
        1 agacaacggc ggcacagcgt ccagtctctc ctggcagcgt cgaagttcgg aactcatcgc
       61 cgattgccgc gtctttcgcg tgcggcgcga cgtgagcgtc agcccgcaca ggggaagcga
      121 gcacgatttc ttcgtcctcg aatcgtccga ctggatcaac atcatcccgc tgacggcgaa
      181 tgatgaagtc gtgatgatcg aacagtacag gcacggctcg gaggaagtga cgttggagat
      241 tccgggtggg atggttgacg cgggcgagtc gcccgcagca gcggcagcgc gtgagatgct
      301 ggaagagacg ggctacgccg cgacgcgcga agtcgtcaac ctcggcaaag ttcgccccaa
      361 tcccgcgatt cataacaact ggattcacac gtttctcgcg cgcaacgtcg aactccggca
      421 aaagcctgtg attgaaagca cggagcacac cgtcgtgcgg ctcgtcccgc tcgcagacat
      481 cccgcgactc atctccgagg gcgcgatcaa tcacgcgctc gtcgtggtag gattccactg
      541 gcttttgctc tccgcagcgc ggctcgcgga gtagaggttt catgtttgat gaagcagggc
      601 tactcggttc tcatcatcga tatgagctac gacgaggcga agaattttgt catggaaggt
      661 ttcccgaccg tcgagttggc gaatgagttt gcgcggcgtt gggtgcgcga ttcggtagag
      721 gagttgcgcg ggttgaaccg gacgcgggag gaagtgcgcc gcttgtggca cacgttcggg
      781 gaagacgcct ccgtgctcgg cggccaaccg cactatgccg gttctcacga actggactac
      841 tttatagagc atccggccac cgccacggag cgcgactggc gggcgataca aaggctcgcg
      901 gggattgaat aaagacaaaa aggtttttgc gattatgcgt gttcaactga caaaactcgc
      961 gcacgcgcgt tcgggcgaca aaggcgacac ggccaacgtc ggcctcatcg ccctgcgcgc
     1021 cgagttctac ccgctgctcg ttcgtgaagt gaccgccgcg cgcgtcaaag agcacttcga
     1081 gggcatctgc aagggcgaag tggaacgctt cgagttggcg aacctcggcg cgctcaactt
     1141 cctcttacac gaatcgctcg gcggcggcgg caccctctcg ctcatgaccg acgcgcaggg
     1201 caagaccttc tccaccgcgc ttctacgcat gcacatcgaa gtgcccgacg aagaagcgcg
     1261 ctcgctcgga ataagctaag tgacgagtgc ataaagcagc gacgagtgac aggtgacgag
     1321 tgacaagtta aaacagcaat cgtgtcttaa gacttatcct taagacttgt cctcgatgtc
     1381 gtcacacggc gcgcacgcga aagtcataaa cgacggacac acttttaact cgttcttgct
     1441 cgtcactcgt cacttgtcac ttgtcactgc cttcttatga gtcgtgaagc cgtcagcacc
     1501 attcccggca tccgccacga ttacgcgcgg gtgggcgagg tgcgcttgca ttacgcggaa
     1561 gcggaagccg gtcgcaggag cgggcgtctg gtgctcttgc tgcacgggtt tcccgaatgc
     1621 tggtattcgt ggcgacatca attgaccgcg ctcggcacac gcttccacgt cgtcgcgcct
     1681 gacttgcgcg gctacaattt gagcgacaag cccgcgcgcg tggaagatta ccgtttggag
     1741 aagttggtgg gcgacgtgct cggcctgatc gagcatttcg gcgcgcggga tgcagcggtc
     1801 gtcggccacg attggggcgc ggcgatagcg tgggagatcg ccgcacgcca tcccgaacgc
     1861 gtgcggaaag tcgcggcatt gcaagtgccg ccgctgccgg tctggttcgc caacatgacg
     1921 ctcaagcaag ccttgagcag ttggtacatg ttcttcttcc agcttcccta tctgcccgaa
     1981 tggcggctcg gcctgaatga tctcggcgga ctcgacggga tattgaaaaa gagttcgcgc
     2041 cccggcacct tcaccgacac agacatcgca gtctataaga acgcgctccg gcaaaaagac
     2101 tcgcgcaccg acaccaccgc cctcaccgcc gcgatcaact actatcgcgc caacctcttc
     2161 tctaatgttt cgcgcttcgg tggcggtgcg aaaggccggg atgcgcgcat ccgcgtcccc
     2221 actctcttca tctatggcga acgcgatttt gccatcgtgc ctgaaaccgt gcgcgatgtc
     2281 ggcaagcaca tcaatgcgcc ttaccgtgaa ctccggctcg ccaacagcaa tcattgggtg
     2341 cagcaggaat cgcccgcgga ggtgaacgcc gcgctcctcg attttctcga atcataaaaa
     2401 acggagtcgt gtccggtcgc aacccacagg cgacgcgctg caaggcagac ggagagagta
     2461 tggttagagg gtcgcaggcc aagtagaaag agtatgaacg agcagcaggc agaactgatc
     2521 tacacggtgg aaggcgcggt tgcgctcctg acgctcaacc gcccggacaa acgcaacgcc
     2581 ttaaacgatg cgctcatcgg cgaattgaaa gacgccctgc ggcgtgctga cgcgagcgaa
     2641 gccgtgcgcg tcgtcgtcat cacgggcgcg ggcgcagact tctgttcggg cgcagacctc
     2701 tccgcgctcc aaaaaatttc cgaaagctcg atcacggaaa atctcgaaga tgcgcagtcg
     2761 ctcgcggaat tgttcgcgct catgcgacgc gtgcgcgtgc cggttgtggc cgccgtgcgt
     2821 ggtcgtgcgc tggcgggcgg ttgcggtctg gcgaccgcct gcgatctcgt gctcgccacg
     2881 cgttccgcgc gcttcggcta cccggaagtg aagatcggtt tcgtgcccgc gatggtcatg
     2941 gcaatcctgc gccgtaacgt ctccgaaaag cgcgccttcg aacttatcac gcgcggcgcg
     3001 gagatcagcg cggaagaggc ggcctcgttc ggcctcgtca atcacgtctt tgcagatgag
     3061 agttttgaga cggaagtgga aaagtacatc gccggattcg agcgcgtgag ccgctccgcc
     3121 gtgatgctca ccaagcgcct tctctatcac atggacggca tgaccttcga cgccgccctc
     3181 caatccggcg cagacctgaa cgccatcgca cgcatgaccg aagactgcca gcagggcatc
     3241 gcgcgctttc tcaaaaagta gatcgcgcag cccgggttaa gtttaaggcc agcggagcag
     3301 gtagtcgcgg agtgtttgat acatgctctc gaaggagtct gcgcaaaaga gaaccggttg
     3361 gtagttggtc gggtcgaact cctgacgcgc cgccgcagcc agatcgagcg ggcgcagctc
     3421 agcctctttc aacgcttcca attctcccgc cgacgagacg agtcctgttc cataggcttt
     3481 cacctcgccc gcttcccgga ccactccgaa ctcgatagtg aaccagtaaa ctcgcgccag
     3541 acgttcgagg tcttccgccc tgcgcgttcg tttgacggct gcgccgacga gacggttgag
     3601 ttcggccaga ggcgggctgg ctaacagcac ggcgtggccg agcagttcgt gcaccacgtc
     3661 cggttcgggc gtgtaaagcg gcgtcgaatg atgacggatg tactgcgtgg agaggaaaac
     3721 gccgctcgcc agagtttcca aaaagacgcg cggctcgacc agcccgccga ccggctcaag
     3781 ccggaaaccg ctgagggctt cgaccttcgc cgacacttcg cccaactgcg gaatccgttc
     3841 gcgcggcaac gccaaccgct cgacgcattc aaggtattcg gcgcaggcgt gtctcaggtg
     3901 ttgcggcctc agaacgtctt gaataatctg ccagagttga tgctcctctt tcgtgtaagg
     3961 cgcatcggga acgggtgaac ccggctgata ttccagagcg atctgcgcga tggcattccg
     4021 gcgcgcgcgg tattcatgat cgcgaaagcc gggatgctcc gggtcgagtt tcaccagtga
     4081 acgcgcgccc tcgttcggag acgaggggac gggcgcgggc tgcgtggtca tctgttcgtg
     4141 aatgcttctc gctgactttt gttcctgtaa cattgtttca actccgtaag tgaccggcga
     4201 aattgggggc aaaaaaaaga accggccact tgtcctcccg catcggaagg aaagtggccg
     4261 gttcaagaaa tccgtcttcg cttatgctac atgcttagca catgccgaca gcccttcgcc
     4321 gccccgtccc tccgtctgga gagcgaaacg taatagtaat agttgcgata ggaactgttc
     4381 ggttgcgtca acatctttgt catctgcgcc ggaaactgtg gcgcgtgaaa tctaaaataa
     4441 gcgcgcggct caaccggtgt caaggtgtcg ccgtcgtctg tgccggttta acccgaacgc
     4501 tccgtaagag agtttggtgt gaactaacgc gcgccccgtc tctcccgcca ttcatcaagc
     4561 gaaggcttgc ccgcgccgtg aaagaggaag aatagtccga cgaagaccat gaggagcgcg
     4621 gggaaggtgc gttgaatcgt gtcgccgcga tgggcgatga aaatggccgc gatgaagttg
     4681 acgataaacg gaatggagat gagacggacg aacgcgccga gcaggatcag gatgccgcag
     4741 acgaactgcg tgtaggccga gaggaaggcc gcgaacaacg ggaagggcac gccgcgcgct
     4801 tcgagaaagt gcgcgaactc ctgcatctgc gcgtagctga aaacgttgtc ctgcacgccc
     4861 cagatgatga acgagccgat gatgagacgg atgaagagcg cgccatactc gcgtcgtcgt
     4921 ttgaagaatg cgaactccaa aagtttagct gccatgctta ctccgtcttg aaagggattg
     4981 aggccggaaa cgaactcgct tgtgccgcgt gtcgctgaac tgcgacgcgc gccgggcaga
     5041 cagtagaacg tgtcgaagca acgcgggcgg atgctacgtc gtgcttcgtt gtgcgtaaag
     5101 ttttttgtga ccggaggaac gttctgcggg acgagccgac gcagtccggg tcggaaacaa
     5161 caacaggcag gcaccaacgc taagactcag gcgcaaacgc taagactcta gccatcccgt
     5221 ctcgcttgca cgcacgcagc ccccgccaaa gccagagcgc attccagcac aggctcaccc
     5281 agacgattca ccaacccgat ctcatacgct tcatgcgccg tgacccggcg cgcggtggtg
     5341 aaaatttcca gcgcgcgggt ttggccgatg agtcgcggga ggcgttgcgt gccgccccaa
     5401 cccgtgatga tgccgagacg cgcgccgggg tgcgcgaaga ccgcgtcggg cacggcgtag
     5461 cgcagacggc aggcgagcgc gaggtcgagg ccgccgccca tgcaatagcc gttgatggcg
     5521 gcgatggtca gttgcggcgc atcagctatc ttttgcatca ggctctggcc gcgcagcgca
     5581 aactccttcg ccgtttcagg ggtgagcgcg cgcagttcgc gtatgttcgc gccggaggca
     5641 aacgagtcgc ccgtgcctgt gaagatgatc gcggagatgt ccgcccgcac gacgagcgta
     5701 gagaccagtg cctctaattc ttcgagcgtg gcgacggaga gggcattgcg ttcggcggga
     5761 cgattgagac ggatgacgac gtgcgaatcg cgcgcttcga cgacgagtgc acgcgtctcg
     5821 actgttggct cctcgtctgg catccgggta atgagagggg cggggaactc aggcgcgcgg
     5881 ctggacgttc tcgcgcagga atttaacgat ctcttcggtg gacgtgccgg gcaggaaaac
     5941 ttcggccacg cccatctcct tcagacgcgg cacgtcctct ctcggcacga tgccgccgac
     6001 gagcacgagg caatcgtcca tcccgcgttc ctgcaaaagc tgcacgatgc gcgggcagag
     6061 cgtgttgtgc gcgccgctca ggatcgagac gccgaccgca tcaacgtcct cctgcaaagc
     6121 ggcggcagcg atcatctccg gcgtctgccg tagccccgta tagatgactt ccatccccgc
     6181 gtcgcgcagc gcacgcgcga tgatcttcgc gccgcgatca tgtccgtcga gtccgggctt
     6241 ggcgacgagt actcgaattt ttctttcact cataagaaga cagtgacagg tgacgagcga
     6301 caagtgacga gccggaatca agatcagttt aaaaacaggg tttctgtttt aacttggcac
     6361 tcgtcacctg tcactcgtca ctgcttttaa aatgcaggct cttcgtagag gccgtaaacg
     6421 tcgcgcaggg cgtcgcagat ttcgccgagc gtcgaatagg cgcggacggc ttcgatggtg
     6481 gcgggcatcg tgttgtcgcc tgcttgcgcc gcgagtttca gtttttcgag agcgtttgcc
     6541 actgcgccgt cgtcgcgacg tgtgcgcgcc tgttcgagcc gcgcgatctg gtgctcgcgg
     6601 acggattcgt ctatgacgag gatgtcgatc ttggaatcgt cgtcgtccat ctggtactta
     6661 ttgacgccga cgatagtttg ctcgccgcgc tcgacggcct tctggtattg atacgccgac
     6721 tcctgaatct cgcgttgcgg aaaacccttc tcaaccgccg aaaccattcc gcccatcgca
     6781 tcgatcttgg caaagtaatc gagcgcgccg tcttccatct tcttcgtcag cgattcgaga
     6841 aagtatgagc cgccgagcgg gtcaaccgtg ttcaccacgc ccgtctcttc ggcgatgatc
     6901 tgctgcgtgc gcagggcgat gagcgcggcc ttgtcggtcg gaagcgcgag ggcttcgtcg
     6961 taagcgtccg tgtggagact ctgcgtgccg ccgagcaccc ccgcgagcgc ttgaatggcg
     7021 acgcgcacga tgttgttcag aggttgctgc acggtgagcg agacgcctgc ggtctgcgtg
     7081 tggaaacgca gttgcagcgt gcgcgggtct ttcgcctgaa agcgttcgcg catcatgcgc
     7141 gaccagatgc ggcgcgcggc gcgatacttc gcaatctctt cgaagaagtc gttatgcgca
     7201 ttgaagaaga aagagatgcg cggcacgaac tcgtccacgt cgagtccggc gtcaacgccc
     7261 cactggacat actcgatgcc gtcgcgcagc gtgaaggcga gttcctgcat cgcggtcgaa
     7321 cccgcctcgc ggatgtgata gccgctgact gaaatcgggt tgtacttcgg cacatggcgc
     7381 gtgcagaact cgaaggtgtc aacgacgagt ttcatcgccg gacggatcgg atagatccat
     7441 tccttctgcg cgatgtactc cttgaggatg tcgttttgca gcgtgccgga aatcttgcgc
     7501 cagtccgcgc cctgcttctc cgccaccacc agatacatcg ccaggaggac ggacgcgggc
     7561 gcattgatcg tctgcgaaac cgtaacctct tcgagcggga tgccgtcgaa gagagtctcc
     7621 atgtcggcga gcgacgagac ggcgacgccg cacttgccga cttcgccttc cgagagcatc
     7681 gcgtcggcgt cgtagcccat gagcgtcggc agatcgtagg cgacggagag gccggtctgt
     7741 ccctgcgcca ttaaatattt gaagcgcgcg tttgtctcct cgggcgtccc gaaccccgcg
     7801 aactggcgca tagtccagag cttgccgcga tagccggtcg ggtgtatgcc gcgcgtgtaa
     7861 ggaaactcgc cggggaaggc tatgtcctta tcgaaatcga tcccgctcgt gtcggcgggc
     7921 gtgtagagac gttcgaccgc atcgagcgag acggtatcaa aacgcttctt gcgctcggga
     7981 cgcttctgca agacgggcgc gagcgtctca tcttcccaac gctcgacggc agcct
//
LOCUS       DASWNS010000209        20525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_371650, whole genome shotgun sequence.
ACCESSION   DASWNS010000209 DASWNS010000000
VERSION     DASWNS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20525
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371650"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VGB76_07040"
     CDS             <1..274
                     /locus_tag="VGB76_07040"
                     /inference="COORDINATES: protein motif:HMM:NF026924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm8 family immunity protein"
                     /protein_id="HEY0098695.1"
                     /translation="RVEIGERGKPGGEVFHFIAASPAGIEGEVGSGEFKLLRGYILMA
                     RFDWAVVYRSIENIINHARGYRDHNWDEVIEFFSRYGRYDSEDIHS"
     gene            805..1266
                     /locus_tag="VGB76_07045"
     CDS             805..1266
                     /locus_tag="VGB76_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098696.1"
                     /translation="MKSILLVEDFEDSRYMMRMLLEMSGYRVLEAADGRRAVELAREE
                     CPDLVLMDLSLPVLDGLSATREIRRMDNISNLPVIALTAHGTEEFQDAALTAGCDEFV
                     TKPIDFDLLESVIREQLGKRGTRACGSVSPFMENTNRVSHLSHKPTRSPEY"
     gene            1377..1655
                     /locus_tag="VGB76_07050"
     CDS             1377..1655
                     /locus_tag="VGB76_07050"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY0098697.1"
                     /translation="MRAIWNNAVLAESDDTVIVEGNHYFPPDSIRSEFLRPSATHTTC
                     PWKGEASYYDVVAGGEVNRDAAWYYPEVKEAAAEIKGRVAFWKGVRVE"
     gene            1686..1895
                     /locus_tag="VGB76_07055"
     CDS             1686..1895
                     /locus_tag="VGB76_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098698.1"
                     /translation="MLRPQPPSAWDAIALRRSMGYVIQEAGLFPHFNVERNVALVPAR
                     IVGRAETARRSGARSRRRSTPLAYG"
     gene            1888..2175
                     /locus_tag="VGB76_07060"
     CDS             1888..2175
                     /locus_tag="VGB76_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098699.1"
                     /translation="MDEPFGALDPLTRAALQNEFAQLRARLDKTVILVTHDVREALRL
                     ATRIALMREGQIVLLATPEEFMRTDEPLARAYLETLQFEDHSPHTDAEDTG"
     gene            2196..2453
                     /locus_tag="VGB76_07065"
     CDS             2196..2453
                     /locus_tag="VGB76_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098700.1"
                     /translation="MNLNEFIKGNWREVSAATGEHLFIVLVSIVLAIIIGIPLGILVA
                     ALRRLVGSITTDEMRLLNYEVDGRRRAIPDVVREWRQREGL"
     gene            2691..3287
                     /locus_tag="VGB76_07070"
     CDS             2691..3287
                     /locus_tag="VGB76_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY0098701.1"
                     /translation="MRSQFNFFDGAGGADETVKHFPEGFRYQPELIGTTVEDALLAHV
                     RELPFREFEFHGYKGKRRVVSFGWRYDFSARHLQKADDIPEYLLSLREAAAAFAGQPA
                     EEFPHVMVTEYSPGAGIGWHRDKGVFGQVVGISLHAPCVLRLRRAVGEKKWERVNVLA
                     EPRSAYFLSGQARSGWEHSIPQVDALRYSITFRTLRRD"
     gene            complement(3434..3958)
                     /locus_tag="VGB76_07075"
     CDS             complement(3434..3958)
                     /locus_tag="VGB76_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0098702.1"
                     /translation="MEFQLDHATEILRRTPATLNSLLRRLPQKWSLANEGVDTWTPFD
                     VVGHLVHGEETDWIPRARLILEYGESRPFEPFNRYAMFEKSRGKSLSDLLDRFEQLRG
                     ESLQELEAMNLKPEMLGKRGMHPELGVVTLSQLLSTWVVHDLGHIDQIVRVMAKQYSE
                     AVGAWQTYLPVLSR"
     gene            4327..4812
                     /locus_tag="VGB76_07080"
     CDS             4327..4812
                     /locus_tag="VGB76_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0098703.1"
                     /translation="MSKNKIDSLSGGSGVHVWLVLWKAAQAVEAYAKKSIDGSGLCGS
                     DFAVLEALLHKGELPVNEIGRKVLLTSGSITVAIDRLEAKGLVERRASADDRRARIVH
                     LTREGRKLITRAYAGHAADMERLASGLSAGERETLIGLLKKIGFKASAALKQKSEDAA
                     G"
     gene            4851..5771
                     /locus_tag="VGB76_07085"
     CDS             4851..5771
                     /locus_tag="VGB76_07085"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0098704.1"
                     /translation="MKVLEVSREEKEIRAKGRTVAGIVNSIETLEGAGFLVRRPFPKA
                     SFSEFDPFLLLDEMGPMKVAPGEAKGAPDHPHRGFETVTYMLSGEMEHKDSQGHAGKL
                     SAGDVQWMTAGAGVVHSEMPSAEFQRAGGTLHGFQLWVNLPRQEKLMRPRYQEIPSAS
                     IPAARTDDGLVAVRVIAGESLGAQAVIETVTPIMYLHFTLQPGGRVSQPLPQGYNAFA
                     YIVDGAGLFGAAGERAGDGQMVMFASDGDKVAIANPAGSGATLELLLIAGVPLNEPVA
                     RYGPFVMNTEEEIYQAVEDFRSGRMGAINF"
     gene            5817..6233
                     /locus_tag="VGB76_07090"
     CDS             5817..6233
                     /locus_tag="VGB76_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0098705.1"
                     /translation="MSQVLTNNSNVRAASAGRITNVVLCVLQVALALMFLMAGFSKLA
                     GAEQMVGVYEQIGTGQWFRYVTGGVEVTSAVLLLVPRLCGLGALLVVATMTGAIFTHL
                     FVIGGSPLIPSVLLFVAAVVAWGRRERTLSLLSSGS"
     gene            6305..7249
                     /locus_tag="VGB76_07095"
     CDS             6305..7249
                     /locus_tag="VGB76_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY0098706.1"
                     /translation="MNRDNSPEQSGAMPGSLETVIVTRVRDLVNGFKVRRVLPSARRR
                     MVGPFIFLDQMGPEILGAGRGLDVAPHPHIGLATVTYLFEGEILHRDSLGTVQPIRPG
                     EVNWMTAGSGIAHSERTPQELRLSGTKLFGVQSWVALPQGAEETAPAFVHHGAEELPI
                     VEGEGKHVRLIAGSLYGARSPMQTLSEMFYADITLAKGARLPVSAEQEERAVYVVEGT
                     VELTGGGRFDAGQLLVFKPGEEITLAAEGAAPARLVLLGGEPMDSPRHIWWNFVSSSS
                     ERIEQAKADWKAGRFAPVPQETEFIPLPESRPVVVRYP"
     gene            7345..7764
                     /locus_tag="VGB76_07100"
     CDS             7345..7764
                     /locus_tag="VGB76_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098707.1"
                     /translation="MSENKSGDIAPAATLKRLSELRRVLLRLHKALLDRERSVYEQVH
                     GRVSSGELLQLVIQHDQFAWLHAISELIVRTDEMLDADEPLTNGEAESLLSKARGLLK
                     LSETEGEFGRKYSAAFQRDPDTVLAHREVMLILSART"
     gene            7925..8815
                     /locus_tag="VGB76_07105"
     CDS             7925..8815
                     /locus_tag="VGB76_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098708.1"
                     /translation="MKRPLRILLQTTIPATEDDWHIGRFSLLREHLSELRDGEGQLLC
                     EVTARDRETNADGDDELLTRLDTAGFDQLWLFAVDAGDGLTEKDCQGITRFRQRGGGV
                     LSTRDHQDLGSSLCTLGGVGRAHFFHTKHEDPDETRRARDDEETKDISWPNYHSGSNG
                     DYQRIIPVEPVHELLRGVSSLSGRVEYFPAHPHEGGVGVPEGESEARVVATGESKVTK
                     RPFNLIVAFEREPDRHGNTLGRAIAESSFHHFVDYNWDTSKGCPSFVAEPPGDGIERE
                     PEKLEDVKTYVRNLALWLAR"
     gene            9097..9534
                     /locus_tag="VGB76_07110"
     CDS             9097..9534
                     /locus_tag="VGB76_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098709.1"
                     /translation="MLTLKSGELALERQQLKPDGRSGYFLLNDKKNYMYVSLFIEPAE
                     ECKDSRSCRDMVWKAGNPAWENPQKVVSSNLGDVYYFEFFMPSFRGQPIEQQHMYAQF
                     VVDGYWVDMHISKVLYKPEDRKLFERIIKSVKFEPKKEAESQK"
     gene            9670..12732
                     /locus_tag="VGB76_07115"
     CDS             9670..12732
                     /locus_tag="VGB76_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY0098710.1"
                     /translation="MQSNRPNTTTRKCRTLLRVLLLGASLACLTPHAHGGTTATPSAP
                     ALRLPDFHAETWTTEQGLPGDTVRAVQQTRDGYLWIATQGGLARFDGVRFQVFNQQNV
                     EEFRTNECSELLEDRAGQLWIGTVGGGLLRYAAGKFTAYGRAEGLPTDVVIRLFEDRA
                     GRLWITSYESLTLFDGNSFKTFNTSDGGRNVYAIPFYEDEAGRVWFFNSRGSSFVNSS
                     TLGFYLDGRLHTGEAAARLFPRKLAVNSGGLPVRDALWAHSPESLTLTRQPFAAGDAP
                     QAQLKYGAGNTPARAYEDAQGRLWIVTTLQELFLLSEGGLRRVTSNAPSRGTINALHA
                     DREGNLWMGTNNGLTRLKAQSFAAYTTADGLAQESPWTIYEDSRGALWVGTSAGLNRW
                     RADGRFDTYTTRDGMAGSAVVSIAEDATGRLWFGSTDGLTSYNEGRFEAYARREGLLN
                     ENVRSVYVDRRGRLWVGTVGGLHLFEAGRFKPYTTADGLAHNSILFIYEDASGSLWFG
                     TPNGLNRWRDERFETFTTKDGLASNIVLGMHETSDGALWLGTLGGGLARFKEGRFKSI
                     TSAAGLGDDTITRVLEDGAGNFWMGSTRGVLRASVKDLNDYADERTRSVTCVAYSKAD
                     GMPSIDCSGGTQPAGWRTRAGTLWFPTAQGIAVIDPARLVRNEVPPPVVIENLVVDRA
                     PVTLADGLRLPRGSRSVEVHFTALSFVDPSKVRFRYMLEGFDRDWVEAGARRDAFYTN
                     LPPGSYRFRVLAANNDGVWSEGGATLAFTLPPYFYQTRWFYLVAAMLTAAAMWGVYQM
                     RVRQLKREFTAVLEERNRIARELHDSLAQGFTSISIHLEAVSAKMGAPHDAAREHLNQ
                     ARLLVRSSLAEARRTVRDLRSELLEAGDLAAALSQVAGQLTAGTNVSTQVTVTGATRQ
                     LPERVESNLLRVGQEALTNAVRHAGARTVRARLEYGGGQVRLRIEDDGHGFDVHTTDA
                     AANGGGFGLRGMRERLTQIGGTLSVESLPGRGTEITATVPLGENGNGE"
     gene            12790..13434
                     /locus_tag="VGB76_07120"
     CDS             12790..13434
                     /locus_tag="VGB76_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0098711.1"
                     /translation="MTEQTNAGGQIRILIADDHPFFLDGLSANLEAEPDMKVVARAAD
                     GRQAVELSREHAPDVILIDLRMPVLNGVEAIREIVACSPASRIIVLTTYDGDEDIHRA
                     LKAGAKSYLLKDVFREELLAAIREVHSGRRHIPAQVAGRLAERAFADELTEREREVLR
                     LIAGGKSNREIGIALSIAEGTVKAHVNNILNKLNANDRTQAAMIALQRGLFRFD"
     gene            13586..13852
                     /locus_tag="VGB76_07125"
     CDS             13586..13852
                     /locus_tag="VGB76_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098712.1"
                     /translation="MSTRLISSRAGLLKVNRPRSQHARLLELLYLACALLLGALLAAT
                     TLSAAPLRRAHTRPHDEHVRQRAATMPRVVHPATRAGVAEFDSK"
     gene            13991..17806
                     /locus_tag="VGB76_07130"
     CDS             13991..17806
                     /locus_tag="VGB76_07130"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEY0098713.1"
                     /translation="MSLVALFAAVNRTGSAQSAGEGERLKLRGQEAFEFLKENEMREP
                     LAAATGAFVQQSRLLAADGASQDNMGFSVAISGDTAVVGAPADAINGKNRQGSAYVYV
                     RSGSNWTQQQKLTSSDGAANDEFGYSVGIVGDTIAIGRHNTQTGVNRTRGAVYVFKRT
                     GATWSEQQILTSNDGVEGDIFGTSLALENDTLIVGAQQKMIGSNFFQGAAYVFTRSSA
                     STSFAQQARLTADDGGFAEFFGYSVAVSGDTAIVGATGLAGGDAASGRGRAYVYVRSG
                     SNWTQQQKLLASDGASGDAFGYSVGVSADTAIIGARADVVGSAGEVGSAYVFTRAGNV
                     WTEQQKLTAAETTPRNDFFGNSVAINGDTVVIGSPAHEFLSGVANHGAVYVFTRTGAL
                     WTRQQKLVHTDVGADALGQSVAFDGTSIIGGAPSKNSARGAAYVFAREPVDPNRINGA
                     QTGQGSFFGRNVDIDGDTAIVSADNDSIDGTFNGTRTGAAYIFTRTGATWTQQARLVP
                     PDGASGDDFGWDVSVSGDTAIVGAFDHKVGANGSQGAAYVFTRAGSVWSFQQKLTTND
                     GSAGDSFGYSIGLDGDTAVISAVGDDILAANDNRGSAYVFTRTGTTWTQQAKLTAPAD
                     KPGGSFGVDVAVSGDTVIAGHSLETIDATPNRGAAYVFTRTGTTWTEQQRLFTATGEN
                     GDGFGVGVSIDGETLVVGAAGFSAVGNKGTAYVYVRANSVWTPQQQLLSNDLDFSDQL
                     GKVVDISGDTIILGAFGKQIGNENSNQGAAYIFKRAGSAWTQTQKLFASDGSQADFFG
                     VSVAVSGDYAIAGAHGDDVEGRQEQGSAYIFALGAVNPTPTPTPTPTATPTPTPTPTA
                     TPTPTPTPTPTPTPTPTPTPTPSPTPTTASVQFSAASYEFNESTESASITITRSGDTT
                     GAASVELRTIDDPAAVPCDPTIKRPDGSSYPQGAAYARCDYSTTIETVSWAAGDAQPK
                     QVAIPLINDGHVEGAEQVELQLVGVQGANAGAPANATLVILDNDTAQSANPIFTTPFF
                     VRVHYLDFLSREPEPNEPWSAILNNCANVNNDPTCDRLLVSQSFLGSLEFQLKGLYVF
                     NFYKVAFNRLPGYEEIIPDMRSVSGRTAEEVYAKRAALPIAFTTRQEFRGLYDALSNQ
                     QYVDALLNRYAIQSINTPDPSNPEGGTKLTLTRAALVARLDTSQGAQALTRAQVLRAV
                     VESDEVRAAEHNRAFVAMQYYGYLRRTPEPAGYDAWLKVINDNPQNLRLMVNGFMNSI
                     EYRLRFGAP"
     gene            18119..18940
                     /locus_tag="VGB76_07135"
     CDS             18119..18940
                     /locus_tag="VGB76_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0098714.1"
                     /translation="MLKKLEPGCSHGKVLKSFDNAGFIMSEIAYSARGRLSEHSHEHA
                     HFCFVLQGTYIESDNKQEIECKPLTLTFRPPGELHKDKFHNQEVRVFTIEIPPRWIER
                     LQQDSIYLGSSSNFQGGVISRLSQRLVREVQRMDTAASLIIEGLTLEIMAETARHSAQ
                     NVERTVPYWLKQAKDLIHARFAENLTLEQIASEVGVHPIHLASVFRQKYLCTVGEYIR
                     GLRIEYACREIERGELPLALIALEAGFANQGHFSSTFKRLTGFTPVAYRNSFRQS"
     gene            19060..20328
                     /locus_tag="VGB76_07140"
     CDS             19060..20328
                     /locus_tag="VGB76_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0098715.1"
                     /translation="MRLVRNIFNCCLASVLLLSLSVATAHAEVIAIKAGKLLNPEAGT
                     TASNQIILVEDRVIKSIGGNITIPLGATVIDLSRYTVLPGLFDAHSHLCFNVRSGQEQ
                     LANTLASPTAYRAIQGVANARAMLDAGFTTVRDVGNNGNYCDTSLRVAIDREIVPGPT
                     MINSGRIIAPFGGQFQIQPERRGLSEPEYFFADTKDELKKAIRENIHYGARVIKIVVD
                     DQPYIYSVDDIKFVVEEARNAGLKVAAHCGTERGARNAAEAGVASIEHGIQMSDETLQ
                     LAKRNNVVLVGTEFTVKLSEYLGLPPDQAKAFHANYVDRLKRAYKIGVPMVFGSDAFF
                     DVEENRAEQALSVIDSFVEAGVANKDLLRYMTTDAARLLGVESRRGAIKVGLRADIIA
                     TTENPLDNILTLKKVAFVMKNGKVFRSPLK"
BASE COUNT         4207 a         6117 c         6366 g         3835 t
ORIGIN      
        1 gagggttgag attggtgaga ggggtaaacc cgggggcgag gtctttcact ttatcgcggc
       61 ctcacctgcg ggcattgagg gagaagtcgg gagcggcgag ttcaaacttc tgcgcggcta
      121 catcttgatg gcgagatttg attgggctgt cgtctacagg tcaatcgaga atattatcaa
      181 tcatgcacgc ggctatcgtg accacaattg ggacgaggtg atcgagttct tcagccgtta
      241 cgggcggtat gattctgagg atatacactc gtagtcttcc caccggcgcg gggttgtacc
      301 gggcatgtca caccttccaa aaactagtga gcacttcgcg ccgggaaaga aaataagccg
      361 acggtcgcag tccgcgaggc tcccccaagc cccccgggca caatcaacca ccggcttatt
      421 tttcgacacc atccggacgc gagacacaac ggcaattccg ttcccccgaa agaatcgctg
      481 ctgtcctgtc tggtcaagtt cagagttccc ccaaaccctt cactcgatcc gccctgatgg
      541 caaagaacgc tctatttcat tccacactat tttttccgac atcacctacg gcaacaggtg
      601 tgccaccgtt cccgccgctg tctgccatta acggggagga tagcgaaagg gctgtattta
      661 tcggcctctc tttatgagta gctggtggag gaatgcggct tgctcagggg gagtccacat
      721 cacgttgggc gcatgatact tttacagccg gactcgtttt gacgagccgg acggtatgga
      781 ttcgatgcaa catcaggttc aagcatgaaa tcaatattac tggttgaaga tttcgaagat
      841 agccgctaca tgatgcggat gctgctggag atgagcggct atagggtttt ggaggcggcg
      901 gacggacgcc gcgccgtaga actggcgagg gaagagtgcc ccgacctcgt cctgatggat
      961 ttgagtctgc cggttctgga cggcctcagc gcgacacgcg agatccgccg gatggacaac
     1021 atcagcaatc tgcctgtcat cgccctcacc gcccacggga cggaggagtt tcaggatgca
     1081 gcgctcacgg caggctgcga cgagttcgtg accaagccta ttgatttcga tctgttggag
     1141 tcggtcatcc gtgagcaact cggcaagcgg ggcacgcgag cgtgtggcag cgtcagcccg
     1201 ttcatggaga acacgaaccg tgtgagccac ctttcccaca aacccacccg aagccccgaa
     1261 tattgagaca ggcaggaatc aagtggtcag tgttggatac atggtcgggg attcttccac
     1321 atctttaatt caccagatat gttccggggc gaagttctga aacacggagg tagaacatgc
     1381 gcgcgatctg gaataatgca gtgctggccg agagtgatga cacggtgata gttgaaggaa
     1441 accactactt cccgcccgat tcgattcgca gcgagttctt acgcccgagt gccacgcaca
     1501 cgacctgccc ttggaaaggg gaggcgagtt attacgacgt ggtggcgggc ggagaggtga
     1561 acagagacgc cgcgtggtat tacccggagg tgaaagaggc tgcggctgag atcaaggggc
     1621 gcgtagcctt ttggaaaggc gtgagagtcg aatagcaagc cgcgccgcga gaggcttcgg
     1681 cgcggttgct tagaccgcaa ccgccgagcg cgtgggatgc tatcgccttg cggcgttcga
     1741 tgggctacgt gattcaagag gccgggctgt tcccgcactt caacgtggag cgcaacgtcg
     1801 cgctcgtgcc cgcgcgaatt gtcgggcggg cagagacagc gcgtcggagt ggcgcgcgct
     1861 ctcgccgccg atccacccct cttgcttatg gatgagcctt tcggcgcgct cgatccgttg
     1921 acacgcgccg cgctccaaaa cgagttcgcg cagttacgcg cgcgtctgga taagaccgtc
     1981 atcctcgtca cacacgacgt gcgcgaagcg ctccgcctcg ccacgcgcat cgcgctcatg
     2041 cgggaagggc aaatcgtcct gctcgcaacg ccggaggagt ttatgcggac ggacgaacca
     2101 ctcgcgcgcg cttaccttga gacgttacaa tttgaagatc attcgccgca cacggacgcg
     2161 gaagataccg gctgaatccc gtttaagcgt ctctcatgaa tctcaacgag ttcatcaagg
     2221 gcaactggcg cgaggtgtcg gcggccacgg gcgagcacct ctttatcgtg cttgtctcga
     2281 tagtgctcgc catcatcatc ggcatccctc tggggattct cgtcgccgcg ctgcgtcgtc
     2341 tcgtcggctc gatcacgacc gacgagatgc gccttctaaa ttacgaagtt gacgggcgca
     2401 gacgcgccat ccccgatgtc gtgcgcgagt ggagacagcg cgaaggactc tgaatgcgcc
     2461 cgttcaaatc agagctacat gaaccgttct taccgaccgt tcgccctccg ggagaggcac
     2521 ggctacccgg tcagcgtttc aatcaaccgt ccgcaagatc agtgcgcgac attgagcggg
     2581 cgggcggaag tgaactccgc ctgagattaa cagtagaatg actttgcgcg gcgcgaagca
     2641 ggcaaccccg gtgcagtatc gcggcgagct ttacgtgtac atagagagct atgagatcgc
     2701 agtttaactt ctttgatggc gcgggcggcg cggacgaaac cgtgaagcat ttcccggagg
     2761 gcttccgcta ccagccggaa ctgatcggca cgaccgtgga ggatgcgctt ctcgcgcacg
     2821 tgcgcgaact gcccttccgt gagttcgagt ttcacggcta caaaggcaaa cgccgcgtcg
     2881 tctctttcgg ctggcgatac gatttctccg cacgtcatct acagaaagcg gacgacatcc
     2941 ccgaatatct cctctcgctg cgagaggccg ccgccgcgtt cgccggtcag ccagcggaag
     3001 agtttccaca tgtgatggtc actgagtaca gccccggcgc ggggataggg tggcaccgtg
     3061 ataagggcgt cttcgggcag gtggttggta tctcgctgca cgctccgtgc gtgctgcgcc
     3121 tgaggcgtgc ggtgggcgag aagaagtggg agcgcgtaaa cgtgttggcc gagccgcgct
     3181 ccgcctactt cctgagcggt caagcgcgtt ccggttggga gcacagcatc ccgcaggtag
     3241 atgcgctgcg ctattcgatc accttccgca cactgcgccg agactgagtg attcgaaggc
     3301 cggtcaggct gctgccgcga gcagtcggcg ggatggtcgt ttaagtaact tgaccctttg
     3361 cagattcccg ttgaaagtag tgctatatgg gagcgggtgc gcgccgtcgt gcgatgcgtt
     3421 gctgcctata caactacctg ctcagaacgg gcagataggt ctgccatgct ccgaccgctt
     3481 cgctgtactg cttcgccatc actctcacga tctggtcgat gtgtccgagg tcatgtacca
     3541 cccatgtcga gagcaactgg ctgagtgtta ccactccgag ttcgggatgc atgccacgct
     3601 tccctaacat ctccggcttc aagttcattg cctccaactc ctgcaaactc tctccacgga
     3661 gttgctcgaa cctgtccagc agatcgctta aagatttacc tctcgacttc tcgaacatgg
     3721 catagcggtt gaatggttca aaagggcgac tctcgccata ctccagaatg agcctcgcac
     3781 ggggaatcca atcagtctcc tccccgtgga cgagatgacc caccacatca aaaggagtcc
     3841 acgtatcaac gccttcattt gccaggctcc acttttgagg gaggcggcgc agcaacgaat
     3901 tcaaagtggc gggagtccga cggagaatct ccgtcgcatg atcaagttgg aattccataa
     3961 ttgactgacc tcgctaacgt cggctttcat aaagataacg cttattgaca caggaagaca
     4021 gcctccatgt tcgccatgcc cgccgcgagg ttaaacgctc tggcagtctt gtcgccaacg
     4081 ccgttccggt tcgcgtcggc catcaacttc atcatgccgc ttccgcttag ttgatctcca
     4141 gacactcgcc ttcaactttg cggcggactt cttgcgctca tccggcgaat cggaagggaa
     4201 gttacgcccg acctaccgga gcgcgtccga ttcttacgag gaaatgataa ccatttaatg
     4261 ccccttgaca attacctgca agtaaagtat cttgatgtca agatacttta ccgatgatga
     4321 gcggcgatga gcaaaaacaa gattgattca ctgagcggcg ggagcggcgt gcacgtctgg
     4381 ctggtgctgt ggaaagcggc gcaggccgtc gaagcgtacg ccaaaaagag catcgacggg
     4441 tcgggactgt gcggcagcga ctttgccgtg ctcgaagcgt tgctgcataa gggcgagctg
     4501 cccgttaacg agatcggcag aaaagttctg ttgacgagcg gctcgatcac cgtggcgatt
     4561 gaccggctcg aagctaaagg gttagttgag cggcgtgcct ctgccgacga ccggcgggcg
     4621 cgaatcgtcc acctgacgag agaaggcaga aagctcatta cgcgcgccta cgccgggcac
     4681 gctgccgata tggagcggct cgcttccggg ttgtcggcgg gtgagcgcga gacgctcatc
     4741 ggactgttga agaagatcgg cttcaaggcg tcggcagcgt tgaaacagaa gtcggaagat
     4801 gcggcggggt gaagtggcaa cggagaattc caaaaggagg gctgtgaaag atgaaagtgc
     4861 ttgaggtgtc gagagaggaa aaagagattc gggcaaaggg acgcacggtg gcaggcattg
     4921 tcaacagcat agagacgctg gagggcgcgg gttttctggt gcgtcgcccg tttccgaaag
     4981 cgtcgttctc ggagttcgac ccgttcctcc tgctggacga gatgggtccg atgaaggtag
     5041 ctcccggtga agccaagggc gcgccagacc acccgcatcg cggcttcgag acggtgacct
     5101 acatgctgtc gggcgagatg gagcataagg actcgcaggg gcacgcgggg aagttgagcg
     5161 cgggcgacgt gcaatggatg accgccggtg cgggcgtggt ccattcggag atgccatcgg
     5221 cggagttcca gcgagcgggc ggcacgctgc acggctttca actctgggtg aacctgcccc
     5281 ggcaggagaa gttgatgcgt ccgcgttatc aggagatacc gtcggcgagc atccctgcgg
     5341 cgcggacgga tgacggtctg gttgcggtgc gcgtgatcgc gggtgagtcg ctcggggcgc
     5401 aagccgtgat cgagacagta accccgatca tgtacctgca cttcacgctc cagcccggcg
     5461 ggcgcgtgtc gcagcccttg ccgcaaggct acaacgcctt cgcctacatc gtggacggag
     5521 cgggcttgtt cggcgcggcg ggagagcgcg ccggagacgg ccagatggtg atgtttgcgt
     5581 ctgacggcga caaggtagcg atagctaatc cggcgggctc cggcgcgacg ctcgaactct
     5641 tactgatcgc cggggttcct ctgaatgagc cggttgcgcg ttacggcccc ttcgtgatga
     5701 acacggagga agagatttat caggccgtcg aagacttcag gagcgggcgc atgggcgcga
     5761 tcaatttctg acacggcagc cgtagagaga cgaacggcgc aaaaggagca ggcgatatgt
     5821 cgcaagtatt gacaaataat agtaacgttc gcgcagcttc ggcggggcgg atcacgaacg
     5881 tcgtcctgtg tgtcttgcag gtcgcgctcg cgttaatgtt tctgatggcg ggcttctcga
     5941 aactcgcggg cgcggagcag atggtcggcg tgtacgagca gatcggcacc gggcaatggt
     6001 tccgctacgt gacgggcggg gtggaggtga cctcggcagt gctgctgctc gtgccgcgcc
     6061 tgtgcggcct cggcgcgctg ctggtagtgg cgacgatgac gggcgcgatc ttcacgcacc
     6121 tgttcgtgat cggcggtagc cctttgatac cgtcggtgct gctcttcgtc gctgccgtcg
     6181 tggcgtgggg gcggcgtgag cgcactttga gtcttctgtc gtcaggcagt tgaggacgcg
     6241 gcgaaagcaa cgggaggcga gcgaagcctc gtttcagggg cggcgagaga cgggtgagac
     6301 gctgatgaac agagacaatt cgcctgagca atcgggagcg atgcccggct cgctcgaaac
     6361 cgttatcgtg acgcgcgtgc gcgaccttgt gaacggcttc aaagtccgcc gcgtgttgcc
     6421 ctcggcgcgt cggcgcatgg ttggtccctt catcttctta gaccagatgg gtccggagat
     6481 actgggtgcg gggcgtggcc tcgacgttgc accgcacccg cacatcgggc tggcgacggt
     6541 gacatatctc ttcgagggcg aaatcctgca ccgcgacagc ctcggtactg tccaacccat
     6601 acgccccggc gaggtcaact ggatgaccgc aggcagcggc atcgctcatt cggagcgcac
     6661 gccgcaggag ttgcgactgt cgggaacgaa actcttcggc gtccagtcgt gggttgcgct
     6721 gccacagggc gcggaggaaa ccgcgcctgc gttcgttcat cacggggcag aagagttgcc
     6781 aatcgtagag ggcgagggca aacacgtgcg cctgatcgca ggctcactct acggcgcgcg
     6841 ctcgcctatg cagacgctct ctgagatgtt ctacgccgac atcacactgg cgaagggcgc
     6901 acgtcttccg gtgtctgcgg aacaggagga gcgcgcggtc tatgtcgtcg aaggtacagt
     6961 cgaactaacg ggcggcggaa gattcgacgc cggacaactg ctcgtcttca agccgggaga
     7021 ggagatcacg ctggcggcgg agggggctgc acccgcccgc ttggtgttgc tcggaggaga
     7081 gccgatggac agcccgcgcc acatctggtg gaattttgtt tcgagttcga gcgagcggat
     7141 cgagcaggcg aaagcagact ggaaggcagg aaggtttgcg ccggtgccgc aggagacgga
     7201 gttcatcccg ctgccggagt cccgcccggt tgtcgtgcgc tacccttaga acgcttcctc
     7261 gtcggagtgt tcttcgagtc gctttaaagt agtctggact gataggcgaa gcatgaaggg
     7321 aagctgagag ttgagggaga agttatgagc gagaacaagt ccggcgacat cgcgcccgcc
     7381 gccacactca aacgcctgag cgaattgcgc cgtgtgctgc tgcgcctgca caaggctctg
     7441 ctcgataggg agcggagcgt ctacgagcag gtacatggca gagtctccag cggcgagttg
     7501 ctgcaactcg tcatccagca cgatcagttc gcgtggctcc acgccatctc cgaactgatc
     7561 gtccgcacgg atgagatgct cgacgccgac gaacccctga ccaacggcga agcggaatcg
     7621 ctgctctcca aagcacgtgg gctgctcaag ttgtcggaga cggagggcga gttcgggcga
     7681 aagtattcgg cggcattcca gcgcgacccc gacacagtgc tcgcgcacag ggaggtcatg
     7741 ctgatcctgt ctgcccgcac atagttttga tgagccgggt cttgaggcac gtttcagaag
     7801 tcggcctcag atgtcaggag actctttgcg tgcgactatg gttgacctgt gaattttaat
     7861 tccgtcgcgg acgcgattcg taataccatt agactctact gagcttgcga taccgtgtga
     7921 gcatatgaaa agaccgcttc ggattctatt gcagaccacc atcccggcca cggaagacga
     7981 ctggcacatc ggcaggttct ccctgctccg cgagcacttg agcgagcttc gagacgggga
     8041 agggcagttg ctctgtgaag tcacggcgcg cgaccgcgag acgaacgcgg acggcgacga
     8101 tgaactgttg acccgcctcg acacagcagg cttcgaccaa ctctggctct tcgcagtgga
     8161 cgcgggcgac gggctgaccg agaaggattg tcagggcatc acgcgctttc gccagcgcgg
     8221 cgggggcgtc ctctccacgc gcgatcatca ggacttaggc tcttcgctct gcacgctcgg
     8281 cggcgtcggg cgcgcacact tcttccacac caaacatgaa gacccggacg agacgcgacg
     8341 ggcgcgcgac gacgaggaga ctaaagacat ctcctggcct aactatcatt ccggcagcaa
     8401 cggcgactat caacgcatca tccccgtcga gcctgtacac gaactcctgc gcggcgtttc
     8461 atccctttca ggccgcgtgg aatatttccc ggcgcacccg cacgagggcg gggtcggcgt
     8521 gccggaaggc gagagcgagg cgcgcgtggt cgccacgggc gagagcaagg tcacgaagcg
     8581 tcccttcaat ctgatcgtcg ccttcgagcg agagccggac aggcacggca acacgctggg
     8641 gcgagccatc gccgagtcga gcttccatca tttcgtcgat tacaactggg acacttcaaa
     8701 gggctgcccg agctttgtcg cggagccgcc gggcgacggg attgaaaggg agccggagaa
     8761 gctcgaagac gtgaagacgt acgtgcgtaa cctcgcgctg tggctcgccc gttaaactcc
     8821 ggtgcgggcg tctgtcccac gtttacacgc ctgagcgcgc cgttgaagcc tgcaaaggga
     8881 tgagcggtca agcgcgtttt cggggcgtgc tcacgtccgt ccgataccct cacgtttgcg
     8941 ggtcacgtcg aaacacaagt caagagaaag gtcacctcta tgctaagacg aatacttcct
     9001 gttttctgct cgttgctggt tctgtctcag gcgtcggcca tagccgcgag tttagacgat
     9061 aagagggttg gccttaccgt cccggcagcc ccctggatgc tgacgctcaa gagcggtgag
     9121 cttgcgcttg aacgacagca actcaagcct gatggacgct ccggctattt cctgctgaac
     9181 gacaaaaaga actacatgta tgtctcattg tttatagagc ctgcggaaga gtgcaaagac
     9241 agcagatcat gccgggacat ggtctggaag gccggtaatc cggcgtggga aaatccgcag
     9301 aaggttgtct catccaacct gggagatgtc tattacttcg agttcttcat gccctctttc
     9361 agaggccagc ccatcgagca gcaacacatg tatgcgcagt ttgtggtaga cggttactgg
     9421 gttgatatgc acatctcgaa agtgctctac aagccggaag accgcaagct gttcgagcgg
     9481 atcatcaaat ccgtaaagtt cgagccgaag aaagaggccg agtctcaaaa gtagtccggg
     9541 cggcgaaaaa atttcttgtc attcttttgt cgtcaaggtg ataatcggcg cgcttcgagg
     9601 gtctgtgcga agatgttcat ttaacccgca cggcgttgat cgtcagacag gcaaaaactt
     9661 ccataatatt tgcaaagcaa ccgccctaat accaccacgc gaaagtgccg gacgctgctc
     9721 agggttctcc tgctcggggc gagtctcgcc tgcttgacgc cgcacgcgca cggcgggacg
     9781 acggcgacgc cttccgcgcc cgcgctccgc ctgccggact ttcatgcaga gacgtggacg
     9841 accgaacagg gcttgcccgg cgacaccgtc cgtgcggtgc aacagacgcg cgacggctat
     9901 ctctggatcg cgacgcaagg cgggctggcg cgtttcgacg gcgtgcgctt tcaggtcttc
     9961 aaccagcaga acgtcgaaga gtttcgcacg aacgagtgct ccgagttgct ggaagaccgc
    10021 gccggtcaac tctggatcgg gacggtcggc ggcggcctcc tgcgttacgc ggcggggaag
    10081 ttcacggcct acgggcgagc cgaagggctg cccaccgatg tcgtgatccg tctctttgaa
    10141 gaccgcgcgg gtcgcctctg gatcacttct tacgagagcc tcacgctgtt cgacgggaac
    10201 agtttcaaga ccttcaacac atccgacggc gggcgcaacg tctatgccat tccgttctac
    10261 gaagacgagg caggacgcgt ctggttcttc aactcgcgcg gctcaagctt cgtcaacagt
    10321 tccacgctcg gtttctatct ggacgggcgt ttgcacacgg gcgaagcggc ggcgcgcctc
    10381 ttcccgcgca agctcgcggt aaactccggc ggcctccccg tgcgcgatgc tttatgggcg
    10441 cactcgccgg agtcgttgac gctcacgcgc cagccctttg cggcgggcga cgcgccgcag
    10501 gcgcagttga aatacggagc gggcaacacg cccgcacgcg cttacgaaga cgcgcagggt
    10561 cgcctctgga tcgtaaccac cctgcaagag ctatttcttc tgagtgaggg cggactccgg
    10621 cgcgtaacct cgaacgcacc gtcgcgcggc acgatcaacg ccctccacgc agaccgcgaa
    10681 ggcaacctct ggatgggcac aaacaacggc ctgacgaggc tcaaagcgca gtccttcgcc
    10741 gcttacacga cagccgacgg gctggcgcag gagtcgccct ggaccatcta cgaagacagt
    10801 cgcggcgcgc tctgggtcgg tacgagcgcg gggttgaacc gttggcgcgc ggatggcagg
    10861 ttcgacacct acacgacgcg cgacgggatg gccgggagcg cggtcgtgag catcgcggag
    10921 gacgcaaccg ggcgactctg gttcggctcg accgacgggc tgacgagtta caacgaaggg
    10981 cgtttcgaag cctacgcccg acgcgaaggc ttgctcaacg agaacgtgcg cagcgtctac
    11041 gtagaccggc gtgggaggct ctgggtcggc acggtcggcg ggctgcatct cttcgaggcg
    11101 ggacgcttca agccctacac caccgccgac gggctggcgc acaacagcat cctcttcatc
    11161 tacgaagatg cgagcggttc gctctggttc ggcacgccga acggcctgaa ccgctggcgc
    11221 gacgaacgct tcgaaacgtt caccacaaag gacggcctcg cgtcgaatat cgtcctcggc
    11281 atgcatgaga cgagcgacgg cgcgctctgg ttgggcacgc tcggcggcgg gctggcgcgc
    11341 ttcaaggagg gcagattcaa gtccatcacc tctgcggcgg ggctgggcga cgacaccatt
    11401 acgcgcgtgc tcgaagacgg cgcgggcaac ttctggatgg gcagcacgcg cggcgtgttg
    11461 cgcgcgagcg tgaaagattt gaacgactac gcggacgagc gcacgcgttc ggtaacctgc
    11521 gtcgcctaca gcaaggccga cgggatgcct tcgattgatt gcagcggcgg cacgcaaccc
    11581 gccggatggc gcacgcgcgc cgggacgctc tggtttccga cggcgcaggg catcgccgtg
    11641 atcgatccgg cgcggctcgt gcgcaacgaa gttccgccgc ccgtcgtcat cgagaatctc
    11701 gtcgttgatc gcgccccggt cacgctggcg gatggcctgc gtttgccgcg cgggagtcgc
    11761 agcgtcgagg ttcatttcac ggcactgagt ttcgtcgatc cgtcgaaggt caggtttcgc
    11821 tacatgctcg aaggcttcga ccgcgattgg gtggaggcgg gcgcgcggcg cgatgccttc
    11881 tatacgaatc tgccgccggg cagctatcgc ttccgcgtcc ttgccgccaa caacgacggc
    11941 gtgtggagcg aaggcggcgc aacgctcgcc ttcactctcc cgccctattt ctaccagacg
    12001 cgctggttct acctcgtcgc cgccatgctg accgctgccg cgatgtgggg cgtctatcaa
    12061 atgcgcgtgc ggcagttgaa acgcgagttc accgccgtac tggaagagcg caaccgcatc
    12121 gcgcgcgaac tgcacgactc gctcgcacag ggcttcacga gcatctcgat ccatctcgaa
    12181 gccgtgagcg cgaagatggg cgcgccgcac gatgccgcac gcgagcatct caatcaggca
    12241 cgcctgctcg tgcgcagcag cctcgcggaa gcgcggcgca ccgtgcgcga tctgcgttcg
    12301 gagttgttgg aggcgggcga tctcgccgcc gcgctctcgc aggtcgcagg acagttgacc
    12361 gccggaacca acgtttcgac gcaggtgacc gttaccggcg cgacgcgcca actgccggag
    12421 cgggttgaga gcaatctgct gcgcgtcggg caagaggctt tgacgaacgc ggtgcgccac
    12481 gcgggcgcgc gcaccgtgcg tgcgcgactc gaatacggcg gcggtcaggt gagacttcgc
    12541 atcgaagacg acgggcacgg cttcgacgta cacacgaccg acgcggcggc gaacggcggc
    12601 ggcttcggcc tgcgcgggat gcgcgagcgg ctcacgcaga tcggcggcac gctctcggtc
    12661 gagagccttc cggggcgcgg cacggagatc acggccaccg tcccgctcgg cgaaaacggg
    12721 aacggcgagt gaagcattaa cttgcaggcg cagagggttc gagtaagaga tcgagttttg
    12781 agatcaacta tgacggaaca gactaacgcg ggcggacaga ttcgcattct catcgcagac
    12841 gatcacccgt tctttctcga cgggttatcg gccaacctcg aagccgagcc ggacatgaag
    12901 gtcgtcgcgc gcgcggcgga cgggcggcag gccgtcgagt tgtcccgcga gcacgcgccg
    12961 gacgtgattc tgatcgatct caggatgcct gtgttgaacg gcgtcgaggc gatccgcgag
    13021 atcgtcgcct gttcacccgc gagccgcatc atcgtgctca ccacctacga cggcgacgaa
    13081 gacattcacc gcgcgctcaa ggccggggcg aagtcctacc tgctcaaaga cgtgttccgc
    13141 gaagagttgc tggcggccat ccgcgaagtg catagcgggc ggcgtcacat cccggcgcaa
    13201 gtggcggggc gtttggccga gcgcgccttc gcagacgagt tgaccgagcg cgagcgcgaa
    13261 gtgctgcgac tcatcgcggg cggcaagtcg aaccgcgaga tcggcatcgc gctctccatc
    13321 gccgaaggca cggtcaaagc gcacgtcaac aacatcctta acaagctcaa cgccaacgac
    13381 cgcacgcagg ccgcgatgat tgcgctccaa cgcggcctct tccgcttcga ctaacccgcc
    13441 gccccacgca ccccgcgact atatctaaag tttacccgcg cgtaggactt ttgcccgatg
    13501 cggaactctc cgcctgccaa ctacaatccg ggacgttgaa catcatctcc cccacacgtc
    13561 aatgcaagac ccggaggaaa tcggtttgtc tacacgtctc atctcttccc gcgcgggtct
    13621 gctcaaagtc aaccgcccgc gcagccagca cgcgcgcctg ctggaactac tctatctcgc
    13681 ctgcgcgctc ctgctcggcg cgctgctcgc tgcgacaact ctctccgccg cgcctctccg
    13741 acgggcgcac acgcgcccgc atgacgaaca cgtccgacaa cgcgcagcaa cgatgccgcg
    13801 tgtcgttcat cccgcaacgc gcgctggcgt cgcggagttc gactcgaagt gagagatcgt
    13861 ccgtccgcat tgtgagagag cgtccgtcca aatctacttg tcaaaccaaa tctacttgtc
    13921 gaagtcttct tcaaaggagt cagaaatctt gaatcgaatt aagaagtccc tcacccttgt
    13981 tttgctcgcg ttgtctcttg tcgccctctt cgctgccgtc aatcgcacgg gttcggcgca
    14041 gtcggcaggt gaaggtgaac ggctgaaact gcgcgggcag gaggcgtttg agtttctcaa
    14101 agagaacgag atgcgagagc ctctagcggc ggcgacgggg gcgtttgtgc aacagtcgcg
    14161 cctgctcgcc gccgacggcg cgagccagga caacatgggc tttagcgtcg ccatcagcgg
    14221 cgacacggcg gtcgtcggcg cgcccgccga tgccatcaac gggaagaacc gtcaaggctc
    14281 cgcttacgtc tacgtgcgca gcggttcgaa ctggacgcag cagcagaaac tcacgtcatc
    14341 agacggcgcg gcgaacgacg agttcggcta ttcggtcggc atcgtgggcg ataccatcgc
    14401 catcggcagg cacaacacgc agacgggcgt gaaccgcacg cgcggcgcgg tctacgtctt
    14461 caagcgcacc ggcgcgacgt ggtcggagca gcaaattttg acttcgaacg acggcgtcga
    14521 aggcgacatc ttcggcacga gcctcgcgct tgagaacgac acgctcatcg tcggcgcgca
    14581 gcaaaagatg atcggctcga acttctttca gggcgcggcc tacgtcttca cacgcagcag
    14641 cgcgagcacg agctttgcgc aacaggcgag actcacggca gacgacggcg gcttcgcgga
    14701 attcttcggc tactctgtgg ccgtctccgg cgacacggcc atcgtcggcg caaccggcct
    14761 cgcgggtggg gacgcagcga gcgggcgcgg gcgggcttac gtctacgtgc gcagcggttc
    14821 gaactggacc cagcagcaaa aacttctggc ttcggacggc gcatccggcg acgccttcgg
    14881 ctactcggtc ggcgtctccg ccgacacggc catcatcggc gcgcgcgcag acgtggtcgg
    14941 ctcagcgggg gaagtcggct cggcctacgt cttcacgcgc gcgggcaacg tctggactga
    15001 acagcagaaa ctcaccgccg ccgagactac tccgcgcaac gacttcttcg gcaacagcgt
    15061 cgccatcaac ggcgacaccg tcgttatcgg ctcgcccgcg cacgagttcc tgtcgggcgt
    15121 cgccaatcac ggcgcagtct atgtcttcac ccgcacgggc gcgctctgga cgcgccagca
    15181 gaaactcgtc cacacggatg tgggcgcgga cgcgctcggc caatcggtcg ccttcgacgg
    15241 cacttccatc atcggcggcg cgccctccaa gaacagcgcg cgcggcgcgg cctacgtctt
    15301 cgcgcgcgag ccggttgacc cgaaccgcat caacggcgcg cagaccgggc aggggagttt
    15361 cttcggtcgc aacgttgata tagacggcga cacggccatc gtcagcgcgg ataacgacag
    15421 catcgacggc acgttcaacg gcacgagaac gggcgcggcc tacatcttca cgcgcacggg
    15481 cgcgacgtgg acgcaacagg cgcggctcgt gccgcccgac ggcgcgagcg gcgacgattt
    15541 cggttgggac gtgagcgtct ccggcgacac ggccatcgtc ggggctttcg accacaaggt
    15601 cggcgcgaac ggcagtcagg gcgcggccta cgtcttcacg cgcgcaggca gcgtgtggag
    15661 cttccagcaa aaactgacga cgaacgacgg cagcgccggt gattcattcg gctatagcat
    15721 cgggcttgac ggcgacacgg cggtgatcag cgcagtgggc gacgacatcc tcgcggcgaa
    15781 cgacaatcgc ggctccgcct acgtcttcac acgcacgggc acgacttgga cgcagcaggc
    15841 gaagctgacg gctcctgccg acaaacccgg cggcagtttc ggcgtggacg tggccgtctc
    15901 cggcgacacg gtcatcgccg ggcactctct ggaaaccatc gacgcaacac ccaatcgcgg
    15961 cgcggcttac gtcttcacac gcacggggac gacctggact gagcaacagc ggctcttcac
    16021 ggcgacgggc gagaacggcg acggcttcgg cgtcggcgtc tcaatcgacg gcgaaacgtt
    16081 ggtggtcggc gcggcgggct tcagcgcggt cggcaacaag ggcacggcct acgtctacgt
    16141 gcgcgccaac tctgtatgga cgccgcaaca acaactgctg tcgaacgacc tcgacttcag
    16201 cgaccagttg ggcaaggttg ttgacatcag cggcgacacg atcatcctcg gcgcgttcgg
    16261 caaacagatc ggcaatgaga attccaatca aggcgcggcc tacatcttca agcgcgcggg
    16321 cagtgcttgg acgcagacgc aaaagctgtt cgcctcagac ggctcgcagg cagacttctt
    16381 cggcgtcagc gtcgccgtct ccggcgacta cgccatagcg ggtgcgcacg gcgacgatgt
    16441 cgagggcagg caggagcaag gctccgccta catcttcgcg ctcggcgcgg tcaatcccac
    16501 gccaaccccg acgccaacgc caacggccac accaactccg acgccaactc ctacagcgac
    16561 gccgacacca acaccgacac caactccgac gccgactcct actccgacac caactccaac
    16621 tccgtcgccg accccgacga cggcgagcgt gcagttcagc gcggcgagtt acgagttcaa
    16681 cgagagcacg gagagcgcgt ccatcaccat cacgagaagc ggcgacacga cgggcgcggc
    16741 gtccgtcgag ttgcgcacga ttgacgatcc ggcggctgtg ccgtgcgacc cgacgatcaa
    16801 gcgtcccgac ggctcttcat acccgcaggg cgcggcctat gcgcgctgcg actactcgac
    16861 gactatcgaa accgtctcat gggcggcggg cgacgcgcag ccgaaacagg ttgccatccc
    16921 gctcatcaac gacgggcacg tcgaaggggc tgagcaggtt gaactgcaac tcgtaggcgt
    16981 gcagggcgcg aacgcgggcg cgcccgccaa cgccacgctc gtcatcctcg ataacgacac
    17041 ggcgcagtcc gccaacccga tctttaccac gcccttcttc gtgcgcgtgc actatctgga
    17101 cttcctctcg cgcgagcctg agccgaacga accgtggagc gccatcctca acaattgcgc
    17161 gaacgtcaac aacgacccga cgtgcgaccg cctgctcgtc tcgcaatcgt tcctcggttc
    17221 gctggagttt caactgaaag gcctctacgt cttcaacttc tacaaggtgg ccttcaaccg
    17281 cctgcccggc tacgaagaga tcatcccgga catgaggagc gtcagcggac ggacggcgga
    17341 agaggtttac gccaagcgcg ccgcgctgcc catcgctttc accacgcggc aggagtttcg
    17401 cggactctac gacgcgctga gcaatcaaca gtacgtagac gcgctgttga accgctacgc
    17461 catccagtcg atcaacacgc ccgacccgtc caaccccgaa ggcgggacga aactgacgct
    17521 gacgcgtgcc gcgctggtcg cacgtctcga cacgtcgcag ggtgcgcagg cgttgaccag
    17581 agcgcaagtg ctgagagcgg tcgtggagtc ggacgaggtg agagcggcgg agcacaatcg
    17641 cgccttcgtc gccatgcaat actatggtta tttgagacgc acgcccgagc cagccgggta
    17701 cgacgcgtgg ttgaaggtca tcaacgacaa cccgcagaac ctgcgcctga tggtcaacgg
    17761 ttttatgaac tccatcgaat atcgtctgag gttcggcgct ccgtaaagct ctggaagaag
    17821 tgtagctcgg agcggaggcc acttgaggca tttaagtctc aagtggcctc tctcttttgc
    17881 gccgtagaag acgcgcacag gcgtctggcc gtgcccccgc cccggtcgct gaagtccatc
    17941 ttttagaaaa ttattgaaac aaaccttctg ccggtggtgt tctaatcctt gaggggcgaa
    18001 aactctgcgg cctgtctttc catgccgtgc aatgtagttg ccgcctgaat atactgtcac
    18061 aaaatatcgt gcccggtgtt gcctgtgtgg aaaacttcga ttccgtcaat ctgaaaatat
    18121 gcttaaaaaa ttagaacccg gttgctcgca cggcaaggtc ttaaagagct tcgataacgc
    18181 gggttttatc atgagtgaga tcgcgtattc ggcgcggggc aggctctcgg aacattccca
    18241 cgagcatgct catttctgtt ttgtcctgca aggcacctac atcgaatctg ataacaaaca
    18301 ggagatagaa tgtaagccgc tcaccttaac cttccggcca ccgggagaac ttcataaaga
    18361 taaatttcat aaccaggaag tacgagtttt cactatcgaa attcccccgc gctggatcga
    18421 gaggctgcaa caggattcga tatatttagg tagttcgagc aattttcagg gcggcgttat
    18481 ctcaaggctc tctcagcgtc tggtcagaga ggttcagcgt atggacaccg ccgccagttt
    18541 gatcattgaa ggtttgacgc tggaaataat ggccgaaacc gcacgccact ccgcacaaaa
    18601 tgtcgaacga acagttccct actggctcaa acaagcgaag gatttaatcc atgcgcgttt
    18661 cgctgaaaat ttaacgctcg aacagatcgc gtcggaagtc ggcgtccatc cgattcacct
    18721 cgccagtgtg ttccggcaaa agtacctgtg cacggtgggt gagtatatac gcgggttgcg
    18781 gatcgaatat gcctgccggg aaatagagag aggcgaactg ccgctggcgt tgatcgcgct
    18841 ggaagcgggc tttgccaatc aaggccactt ttccagcacg ttcaaacgtc tcacaggctt
    18901 cacgcccgtc gcatatcgaa attctttccg ccaatcttaa gcaaagataa aaagtcttaa
    18961 gttttcgtaa gacaggcagg ttgaagcgat tcagaatggg acccaggagc gtccgtctca
    19021 aactaactaa ctgcggtttg cctcgaaaaa ggagatcaga tgagattggt gagaaatatt
    19081 ttcaactgct gtctggcctc tgtgctgctg ctgtctctct cggtcgcgac ggcacacgcg
    19141 gaagttattg cgatcaaagc cggaaagctg ttgaacccgg aggcgggcac gacggcttcg
    19201 aaccagatca ttctggtcga agatagagtc atcaagtcga taggcggcaa catcacgatc
    19261 cctttgggcg cgactgtcat agacctttcc agatatacag tcctgcccgg cctgttcgat
    19321 gcacattcgc atctctgttt caacgtcagg tcagggcagg agcaactagc caacacgcta
    19381 gcgagtccca ccgcttaccg tgcgattcag ggagtggcga acgcgcgcgc gatgctcgac
    19441 gccggtttca ccaccgtccg cgacgtgggg aataacggca attattgcga tacatctctg
    19501 cgcgtggcga tagacaggga gatcgttccg gggccgacga tgatcaattc cgggcgcatc
    19561 atcgcgcctt tcggcgggca atttcagatt caaccggaac ggcgcgggct gtcggaaccc
    19621 gaatactttt tcgccgacac gaaggatgaa ctcaagaaag ccatacgcga aaacatccac
    19681 tacggcgcgc gtgtgatcaa aatagtcgtc gatgaccaac catatatcta ctcggttgac
    19741 gacataaaat ttgttgtcga agaggcgcgc aacgccgggt taaaagttgc cgctcactgc
    19801 ggaactgagc gcggcgcgcg caacgctgcc gaagccggag tcgcgtccat cgaacacggg
    19861 attcagatgt cggatgagac gttgcagcta gccaagcgaa acaatgtggt gctcgtcggc
    19921 acggagttta ctgtcaaact gagcgagtat ttagggctgc ctcccgatca agccaaagcg
    19981 tttcatgcta actacgttga ccgcctgaag cgtgcctaca aaataggcgt gccgatggtt
    20041 ttcggctcgg atgcgttctt cgacgttgaa gaaaatcgtg cggagcaggc gctttccgtc
    20101 attgatagtt tcgtcgaagc cggagtggcg aataaagacc tgcttcgtta tatgacgaca
    20161 gacgcggcgc gcttgctcgg agttgaatcg aggcgcggag cgatcaaagt aggcttgcga
    20221 gccgacatta tcgccacaac ggagaacccg ctcgataaca ttctgacgct caaaaaagtc
    20281 gctttcgtca tgaagaacgg caaggttttc agaagcccgc tcaagtagcc ggagtccact
    20341 cgtaggccga cccgctaacc cggcttcact tttgaaccca ccggagaaca ggcagaagaa
    20401 gtctggtcag cacggcttcc ggcctctgct gatctccaga gttgaacggg gcttcacgct
    20461 ggttgttatc agtcgcaagc ggagattagc gagcgcagta tttgcggaag agaagaggcc
    20521 gcctg
//
LOCUS       DASWNS010000210         4809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_191596, whole genome shotgun sequence.
ACCESSION   DASWNS010000210 DASWNS010000000
VERSION     DASWNS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191596"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VGB76_07145"
     CDS             <1..338
                     /locus_tag="VGB76_07145"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY0098716.1"
                     /translation="ATLYHVDFADDERGWAVGERGTILRTVNGGETWSSVSVPGVQRT
                     TLLSVRFAGEDDGWIVGRGGVILRTEDGGRTWLRQESGTKQNLYALFVNKKQNWAVGG
                     DGMVLRYER"
     gene            416..715
                     /locus_tag="VGB76_07150"
     CDS             416..715
                     /locus_tag="VGB76_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098717.1"
                     /translation="MLKDALEDFRRTIETNAARLHALSEAETRRARGEAGDGWTPKQI
                     AGHLIDSASNNHGRVETKAKAAKGGVGRTFAALAEHQCAAVTRHARRLTSCLESP"
     gene            complement(690..2699)
                     /locus_tag="VGB76_07155"
     CDS             complement(690..2699)
                     /locus_tag="VGB76_07155"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY0098718.1"
                     /translation="MPKFLLFMVALVAVGASGCVQQPAQQNTNTAQAPASAGGDQDFK
                     KIHDAYVREFLRRNPTVNTYLGGAGLDPALREVDGQLRDHSAASIEAEDRWLAETQKS
                     LEAVAPGTLSPNLRIDREVALAQIRFLLRQHGTRKYQQRALDTYTDEPFRAIDWQLQG
                     MTQTGEKSYGTPEEWSLVVKRLQAVPKYMAVAQEQLAAGVKSGNPPDHRMLLRNGINT
                     SEANAEYFEKTLPTLAGERIAEGAGREELWKQLDEAAKGAAGAYRNLRAYVAQSFFDD
                     VNKPGAGNVKTQFRADRYAMGEAEYDWAIKNNLRMPDATAAKLFDESMEIVSSTQQEM
                     ITLAREIGKKKTMELPEAGEAAVLKVFEELGKDYPKSDAEMVRWYEEAAFRLVEYGRK
                     SGVFDVPADYKLEVVETPPPLRASIDGAAYYPAPPFKTSGVGRFYVTTSGTNDEAALR
                     ENNRASLADLSAHEGFPGHDWHYKVMTQYREGIAPVRWLTPGAVEDSSSMWQDSMASE
                     GWALYSEALMAEPQPGYAAGFYSPEERLYQLKGKLYRDLRVRVDTGIHTGRMTYDEAV
                     DLFSQIVDFQPGSCRESNPAQTPAKRASCKTAEAAIFRYSKWPTQAITYRLGKDQIFA
                     LRKDAAQSLGERFAPKDFHILFMKQGTIPVGYFREELLREIQGKK"
     gene            complement(3010..4782)
                     /locus_tag="VGB76_07160"
     CDS             complement(3010..4782)
                     /locus_tag="VGB76_07160"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0098719.1"
                     /translation="MSQTETELETFRREPPAPLAPRPLNLPVPEETTLANGLRIVFVE
                     NTRLPFVTYRLAFRTGDAHDPPNLSGLTDLLTGMLNEGTTSRTSRQIADAVARIGATL
                     GAGASSDNTTVAASALAQFGDEILELMADIVLRPSFPADELELTKQNALQNLIAQRGQ
                     PSFLANERLARVIYGEHPYSVVSATPESIVAISQDDLIEFHRRRFVPNNAVLVCGGDI
                     RRDATLARLEELFGAWQPGEPAESNFPAPPVRERRTLYLVDRPGSAQSNIVIANTAIT
                     RTDPDYFPMLVMHTILGANASSRIFMNLREDKGYTYGAYTSLDARRTAGSFRATAEVR
                     TPVTGESLKEFFYELGRIREELVTEKELQDAKSYLTGIFPIRLETLDGLIDQLIQIKM
                     HDLPDDYLQTYRERVSAVTREEVQRVARRFVTPDRAAIVVVGDADALADELKDYADDF
                     EIYDSAGQRRKDATPEAEAETTQNGRDMSSTTPAGDNSGFVGSWQLEITSPLGPQTAT
                     LVVDEDGAGALRGKLSSSWGEAPLDSLRQNDGIFQAAVALKIQGMSIQANIEGRREGD
                     NLSGTIRTNMPIVPPLMFTGRKIS"
BASE COUNT          857 a         1396 c         1589 g          967 t
ORIGIN      
        1 acgcgactct ctatcacgta gacttcgcag acgacgagcg cggctgggcg gtcggcgaac
       61 gcggcacgat cctgcgcacc gtgaacggcg gcgagacgtg gagcagcgtg agcgtgcccg
      121 gtgtgcaacg cacgacgctc ctcagcgtgc gcttcgcggg cgaggatgac ggctggatcg
      181 tcgggcgcgg cggcgtcatc ctgcgcacgg aggacggcgg gcgcacgtgg ctgcggcagg
      241 agagcggcac gaaacagaac ctctatgcac tcttcgtcaa caagaaacag aattgggcgg
      301 tcggcggcga cggcatggtc ttgcgttacg agcggtagcg gagcagtgac aagtgacaag
      361 tgatggctga gaagtgacga gataaagaga agcgtgggaa atgaaggagt gtgcgatgtt
      421 gaaagacgcg ctcgaagatt tccggcgcac gattgagacg aacgccgcgc gtctacacgc
      481 cttgagcgaa gcggagacgc gacgtgcgcg cggcgaagcc ggggacggct ggacgccgaa
      541 acagatcgcc ggccatctga tagactcggc ttcaaacaat cacggtcgag tagagactaa
      601 agccaaagcc gcgaagggcg gcgtcggtcg caccttcgcg gctttggctg agcatcaatg
      661 cgcggctgtc acacggcacg cgcgtcgcct cacttcttgc cttgaatctc cctgagcagt
      721 tcctcccgaa agtagccgac ggggatcgtg ccctgtttca taaagaggat atgaaaatct
      781 ttgggcgcaa agcgttcgcc tagagactgc gctgcgtctt tgcgcagggc gaagatttga
      841 tctttgccga ggcgataggt gatggcctgc gtcggccact tggaataacg aaagatggcg
      901 gcttcggcgg tcttgcaact cgcgcgcttg gccggagtct gcgccggatt agactcgcgg
      961 cacgaaccgg gttggaagtc cacgatctgc gagaagagat cgacggcttc gtcgtaagtc
     1021 atgcgcccgg tgtggatgcc tgtatcgacg cgcacgcgca ggtcgcgata gagtttgcct
     1081 ttgagttgat agaggcgttc ctctggcgag tagaagcccg ccgcgtagcc gggctgcggt
     1141 tcggccatga gggcttcgga gtagagcgcc cagccttcgg aggccatcga atcctgccac
     1201 atcgaagacg agtcttcgac cgcgccgggc gtgagccagc gcacgggcgc gatcccttcg
     1261 cgatactgcg tcatcacctt gtaatgccaa tcgtggccgg ggaagccttc gtgcgcggag
     1321 aggtctgcga gcgaagcgcg gttgttctct ctgagcgcgg cctcgtcgtt cgtgcccgaa
     1381 gtggtgacgt agaaacgacc gacgccgctc gtcttgaaag gcggcgcggg ataataggcc
     1441 gcgccgtcaa tcgaagcgcg caggggcggc ggcgtctcta cgacttccag cttgtagtct
     1501 gcgggcacat cgaagacgcc ggacttgcgc ccgtattcga cgagacgaaa cgcggcctct
     1561 tcgtaccacc tgaccatctc cgcgtccgac ttcggataat ccttgccgag ttcctcgaag
     1621 accttcagca cggcggcctc gcccgcttcg ggcagctcca tcgtcttctt cttcccgatc
     1681 tcgcgcgcca gcgtgatcat ctcctgctgc gtcgaactca caatctccat cgactcgtcg
     1741 aacaacttcg cggcggtcgc gtccggcatg cgcaggttgt tcttgatcgc ccagtcgtac
     1801 tcggcctcgc ccatcgcgta gcggtctgcg cgaaactgtg tcttgacgtt acccgcaccc
     1861 ggtttgttca catcatcgaa gaaactctgt gcgacgtaag cgcgcaggtt gcgatacgcg
     1921 cccgccgcgc ccttcgcagc ctcgtccaac tgtttccaca actcctcacg ccccgcgcct
     1981 tcggcgatcc gttctccggc gagcgtgggc agcgtcttct cgaaatattc ggcgttggct
     2041 tcggacgtgt tgatgccgtt gcgcagcagc atgcggtggt cgggcgggtt gccggatttc
     2101 actccggcgg cgagttgctc ctgcgcgacc gccatgtact tcggcacggc ttgcagccgt
     2161 ttgacgacga gcgaccattc ttcgggcgtg ccgtaacttt tctcgcccgt ctgcgtcatg
     2221 ccttgcagtt gccagtcaat cgcgcggaag ggttcgtccg tgtaggtgtc gagcgcgcgc
     2281 tgctggtatt tgcgcgtgcc gtgttgacgg agcaggaagc gtatttgcgc gagcgcgact
     2341 tcgcggtcga tgcgcaggtt gggcgagagc gtgccggggg cgaccgcttc gagcgacttt
     2401 tgcgtctcgg cgagccagcg gtcttccgct tcgattgacg cggcggagtg atcgcgcagt
     2461 tgaccgtcca cttcgcgcaa ggccggatcg agtcccgcgc cgccgagata ggtgttcacg
     2521 gtcgggttgc gccgcaaaaa ctccctgacg taagcatcat gaatcttctt gaaatcctga
     2581 tcgccgcctg cgctggcggg cgcctgcgcc gtgttcgtgt tctgctgcgc gggttgctgc
     2641 acgcagcctg acgctccgac ggcaacaagc gcaaccatga ataatagaaa ttttggcatc
     2701 gttcgtctcc agttgaaaac gctgtaggaa gttgaaaaaa cgggtggggc gagtttgaaa
     2761 aatagggggc aaccagccgc ccgtacgccc gcatctgaac aggcgagcat agcaatgacc
     2821 caatgaatcg ccggggggcg attcgcagag ttgggcaatt ctcctgtggt ttccgcccgg
     2881 tgtgagagca cacaccgggc ggttttttgt ttggtcgcta gccgttggct gtcggcaaaa
     2941 tcaggtctga gttgttcagc tttaatctca tctccggcaa cctgcgtatg agccgctagc
     3001 ggctgacagc taactgatct ttcgcccggt gaacatgagc gggggcacga tgggcatgtt
     3061 ggtgcgaatt gtgccgctca ggttgtcgcc ttcgcggcgg ccttcgatgt tggcctgaat
     3121 cgacatgcct tgaatcttga gcgcgacggc ggcctgaaag atgccgtcgt tttgcctgag
     3181 gctgtcgagc ggcgcttcgc cccacgacga actcaatttg ccgcgcagtg cgcctgcacc
     3241 gtcctcgtcc acaacgagcg tcgccgtctg tggcccgagg ggcgaagtga tctccagttg
     3301 ccacgaaccg acgaatcccg aattatctcc tgctggtgtt gtgctgctca tatctctccc
     3361 gttctgtgtg gtttcagctt cagcctcggg cgtcgcatct ttccggcgtt gccccgcgct
     3421 gtcataaatc tcgaagtcgt ctgcgtaatc tttaagctcg tcggcgagcg cgtcggcatc
     3481 gccgacgacg acgatggccg cgcggtcggg cgtgacgaac ctgcgcgcga cgcgctgcac
     3541 ctcttcgcgc gtgactgcgc tgacgcgctc ccggtaagtc tgcaaataat cgtcgggtag
     3601 gtcgtgcatc ttgatctgga tcaactggtc gatcaaaccg tcgagcgtct ccagacgaat
     3661 ggggaagatg cccgtgagat aactcttcgc gtcctgcaat tccttctcgg tgacaagctc
     3721 ttcgcggatg cgtccgagtt cgtagaagaa ctccttgagc gattcgcctg tgacgggcgt
     3781 gcgcacttcg gcggtggcgc gaaaactccc ggcggtgcgg cgcgcgtcga gagaggtgta
     3841 tgcgccgtaa gtgtagccct tgtcttcgcg caggttcatg aagatgcgcg aggaagcgtt
     3901 tgcgccgagg atcgtgtgca tcacgagcat cggaaaatag tcggggtcgg tgcgcgtgat
     3961 agccgtgttg gcgatgacga tgttcgactg cgccgagccg ggacgatcca ccaggtagag
     4021 cgtgcgccgc tcgcgcacgg gcggggcggg gaagttcgat tcagcaggtt cgcccggttg
     4081 ccacgcgccg aagagttctt cgaggcgtgc gagcgttgcg tcgcgccgga tgtcgccgcc
     4141 gcagacgagc acggcgttgt tcgggacgaa gcgacgccgg tgaaactcta tgagatcgtc
     4201 ctgcgagatg gcgacaattg attcgggcgt ggccgaaacg acgctgtaag gatgctcgcc
     4261 gtagatgacg cgcgcgagtc gttcgttggc gagaaaggag ggctggccgc gctgcgcgat
     4321 caagttctgc aacgcgttct gtttcgtcaa ctccaactcg tcggcgggaa acgagggacg
     4381 cagcacgatg tcggccatca actctaaaat ttcgtcgccg aactgcgcga gcgcggaagc
     4441 cgcgaccgtg gtgttatcgg aactcgcgcc cgcgccgagc gtcgcgccga tgcgcgccac
     4501 cgcgtctgcg atctggcggc tggtgcgcga ggtggtgccc tcgttgagca tcccggtcaa
     4561 caggtctgtg aggccggaca aattgggcgg gtcgtgcgcg tcgccggtac ggaaggcgag
     4621 acgataggtg acgaacggca gccgcgtatt ctccacgaag acgatgcgca ggccgttggc
     4681 gagcgtcgtc tcttcgggca cgggcaggtt cagagggcgc ggcgcgagcg gggcaggcgg
     4741 ctcgcggcga aaagtttcga gttctgtttc ggtctggctc atatgaatac agtgacgagt
     4801 gacaagtga
//
LOCUS       DASWNS010000211         9823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_371785, whole genome shotgun sequence.
ACCESSION   DASWNS010000211 DASWNS010000000
VERSION     DASWNS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371785"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VGB76_07165"
     CDS             complement(<1..295)
                     /locus_tag="VGB76_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0098720.1"
                     /translation="MTEEQEVFHRHIQRAGLKRTAQRDLILEVFLRTEEHLSSEDLYR
                     LVQKEDPEIGQTTVYRTLKILTDAGLARAVRFGDGRTRYEHNYKHPHHDHMICT"
     gene            426..511
                     /locus_tag="VGB76_07170"
     tRNA            426..511
                     /locus_tag="VGB76_07170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:459..461,aa:Leu,seq:taa)
     gene            561..932
                     /locus_tag="VGB76_07175"
     CDS             561..932
                     /locus_tag="VGB76_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098721.1"
                     /translation="MTGDEMERAIEFLLQSQANHEARLGELTDRVGNLTEKTDQLTEQ
                     VAETNRLVRLQGEMQTQFIQIVTRHIEAQGEINASLRVAINQADAKIKQADARITNTD
                     ARLDRLEALIERYIEGRNGQG"
     gene            complement(1001..2152)
                     /locus_tag="VGB76_07180"
     CDS             complement(1001..2152)
                     /locus_tag="VGB76_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0098722.1"
                     /translation="MLTLIKNGEVYAPASLGRQDVLLFDRRIAAIGQIDAATLAPLKL
                     ELEVVEASDCLVTPGLIDPHQHLLGGSGEKGFSTQTPEVSASELALAGITTVVGCLGV
                     DTTTKTMTGLLAKAKGLNEENLTAYVWTGGYNVPPTTLMQGVREDIICIAEVIGAGEV
                     AIADERANAHDAPELAKVVKDAYVGGLLSRKCGVTHFHVGEGKSRLKLLRALIDEYEI
                     NPALLYPTHVERDEALMREAIELATRGSYVDLDTVEQDLPKWLRFYLDNGGDAARLTV
                     SSDASINSPRSLLNQIRACVLEHNFPLAQMLAHVTINPASVLQLKQKGRLETGRDADL
                     LVLRKDSLEIKDVIACGRRLVSDGKLAFSENFLSETNRLIRLEGKKSND"
     gene            complement(2162..4822)
                     /gene="cphA"
                     /locus_tag="VGB76_07185"
     CDS             complement(2162..4822)
                     /gene="cphA"
                     /locus_tag="VGB76_07185"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY0098723.1"
                     /translation="MRVDKIRTLAGANIYNHKPVLVMKLYLEELAETESLEISGFNER
                     LISLLPGLDEHHCNKGRAGGFVERLSTGTYFGHIVEHVALELSGMAGVPVYYGKTIYA
                     DEPGCYNVIVAYKAEHGMHYLLEVAVELVDALVKGDSYPLAEKLEEARRIIARTELGP
                     STRAIIDAARRRDIPWFRVNSDSLVQLGYGKNRKFIQAAMTSATGGIAVEIAGDKELT
                     KTILEQADLPVPRGRVVETADEAIAVLSDFAAPVVVKPMDGHQGKGVSLNLTTPIEVF
                     EAFKIAQAYSHDVLIEEQLVGRDYRVLVVNGKLVAASERLPCHVVGDGEHTLAGLIEL
                     VNQDPLRGEGHDKVLTQIKVDEVMLSSLLKRGLSLESIPAAGELVLLRETANLSTGGT
                     ATDVTDLVHPETRRACERAARIVGLDICGLDLVAADISQPLQKGDGFIEANAAPGLRM
                     HTAPTHGQPRDVGGAILDMLFPHDTQARIPIISITGTNGKTTVTRMIGHVLQEMRWCV
                     GMTTTDGIHIDGHVVSRGDTTGPHSARVVLSDPTVEVAVLETARGGIARRGLGYDWSD
                     ISVMTNIREDHIGQDGIRSIEDLVHIKSLIAERVREGGTLILNADDEQLVQLIENPRV
                     RRTDKQFIYFSLNDNNLLIRKYLGIGGGAFFVRDGWIMEAVGREEHRVVEVSAIPVTM
                     SGTARFQVANAMAAIAACRAYGVTRENVAAALKTFRNDQHNPGRANLYQVGEGYVMVD
                     YGHNSDAFEAVCRMAALWSGRHVTAILGVPGDRDDALIETAGRVAARGFHRIIIKEDK
                     DLRGRRKGEVAGVLCRAVNEASPDRECRVVLDEIAAFSRELNEMKEGDVLVIFYDRLE
                     PVLEVLEQHGAVPVTSIGEFALPQLSVATN"
     gene            complement(5077..5994)
                     /locus_tag="VGB76_07190"
     CDS             complement(5077..5994)
                     /locus_tag="VGB76_07190"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEY0098724.1"
                     /translation="MTKEKTADDELTDGGNNGARATGGAKKRAGTKKKATRKTDADSK
                     TALSKTSNTTRAVGRKKVIDDGVDRETIRQRPEELPLMEHHWGKLLFMHWPISPERLR
                     SLIPPQLTIDTFDGEAWIGVTPFTLWDVRLSFTPPVPYLSDFHELNVRTYVLYRGVPG
                     VWFFSLNTNSSMTVFGARTFYFLPYFNARIDLRQEGDTIHYDLERTDADRPARFKASW
                     EIGRELPESEPGTLEFFLTERYCLYSASGESIYRARINHRPWPLRQAKLISHESDIVE
                     SDYLPKLKGKPLVHYAESLAVDIWLPEKA"
     gene            complement(6032..6235)
                     /locus_tag="VGB76_07195"
     CDS             complement(6032..6235)
                     /locus_tag="VGB76_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098725.1"
                     /translation="MATKKSSGKRELIDTGTDKRYVRRDDEGRFSESDDVGRSLSQDV
                     KRKAKTTVKPGQGDKGDQKRGKK"
     gene            complement(6283..7896)
                     /gene="ligD"
                     /locus_tag="VGB76_07200"
     CDS             complement(6283..7896)
                     /gene="ligD"
                     /locus_tag="VGB76_07200"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY0098726.1"
                     /translation="MGLEVYQRKRDFRRTPEPKGTVSKRNLRRFVVQEHHAEKLHFDF
                     RLEMGGVLKSWSIPRGPSLDPAEKRLAVPTEDHPVEYLKFEGHIPEGNYGAGEHMRWD
                     GGTYELVGDGDALAQYEAGRLKFRLHGEKLRGEFSFVRMGHWKGQWLLIKSSDEFAET
                     GWQLQLRQPDDERGAAWQREQGAKRQKRAAAVVAEHKAVKRVEPPKDVETTGAQRFFA
                     RKRLSGNVNVKIGEAVVALTNLDKVYWPEDGYTKGDLVRYYYEIAHYLSPYLAGRPLI
                     MKRYPAGIGGGSFHQHDVNEAPEFVATAEIDVEEGHAVDYIVGGERPTLVYMANLAAI
                     ERHPWHSRIEQLDRPDWVVFDLDPENVDFKIVCETAVSTRDILNGLGLESYAKTSGSR
                     GLHIYVPIKAVHDYAAAARFAELVAKLVVRENSAVATVERSLKKRQHARIYVDHMQNA
                     RGKSVVAPYSVRPRPGAPVSAPLSWEEVERQQIHPQDFNIKNISSRLARTGDLFKPVL
                     NGRQTLDEALLELESMSGKRKRKSTRTGA"
     gene            8069..8704
                     /locus_tag="VGB76_07205"
     CDS             8069..8704
                     /locus_tag="VGB76_07205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0098727.1"
                     /translation="MGHAILLGDSVFDNAAYVGAGDPDVVRQLQEQLPHGWQATLLAV
                     DGSVTRNIRAQAERLPTGSSHLILSVGGNDALGYARLLEAPARSMAEALTMLARAGDD
                     FEREYQLMLEELLSRRLPTAVCTIYNPRFTEAQRQKLAVTALCIFNDAIIRAAFARGL
                     PLIDLRLVCNDDRDYANPIEPSARGGAKIAAAIKRVVTEHDFSRGRTEGFV"
     gene            8832..9077
                     /locus_tag="VGB76_07210"
     CDS             8832..9077
                     /locus_tag="VGB76_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098728.1"
                     /translation="MTTKPNTGTQDTTYNLLSIIYHALQGAETYEKYINDAEQHGDRE
                     LAQYFRDVRDQNIKRAEQGKELLSARLGQDQAQGQSA"
     gene            complement(9214..9534)
                     /locus_tag="VGB76_07215"
     CDS             complement(9214..9534)
                     /locus_tag="VGB76_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0098729.1"
                     /translation="MEEITATELKERLDRGDDLQIIDVREPNEYEAARLEGSKLIPLG
                     QIVTRKGEIDESRETVVHCKMGGRSAKAIEALRGAGFGGRLLNLKGGITAWSNDVDPN
                     VKKY"
     gene            complement(9583..>9823)
                     /locus_tag="VGB76_07220"
     CDS             complement(9583..>9823)
                     /locus_tag="VGB76_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098730.1"
                     /translation="IRALIYYPMSLGRNIDEILRAVDGLQTADANACATPANWRPGDQ
                     VIVPPPQTIADAEKRLQSEYDVTDWYFAKRTLEKS"
BASE COUNT         1759 a         3037 c         2937 g         2090 t
ORIGIN      
        1 cggtgcagat catgtgatcg tgatgcgggt gtttgtagtt gtgctcgtag cgcgtgcgcc
       61 cgtcgccgaa gcgcacagcg cgcgccagtc ccgcgtcggt caaaattttg agcgtgcgat
      121 agacggtggt ttgaccgatc tccgggtctt ctttctgcac gagccggtag aggtcttcgc
      181 tcgacaggtg ttcctcggtg cgcaggaaga cttcgagaat gaggtcgcgc tgcgcggtac
      241 gttttaaccc ggcgcgttga atgtggcgat gaaaaacttc ttgctcttcg gtcatatcct
      301 ttgccggtgg cacagttgaa actcgctcat tgatgattgg cacgagaggg attttaacat
      361 tagcgcgagg ctgtgtgaag aagttggttg cgcgcgcgcg tccgtgctat cttttcgcgc
      421 atggtgccgg ggtggcggaa tggcagacgc gacggactta aaatccgttg gccgtaaggc
      481 cgtgagggtt cgagtccctc cctcggcacc agacaagaac gcctcatctt taagcagcac
      541 cacggaaagt gaggagttac atgaccggcg acgaaatgga gcgtgccatt gagtttctct
      601 tacaaagtca ggctaatcat gaagcgcggc ttggcgagtt gacggatcgg gttggcaact
      661 taaccgagaa aactgatcaa ctgacggagc aggttgcgga gactaatcgg ctggtgcgac
      721 ttcaagggga gatgcagacc cagtttattc agatcgtaac gcggcatatt gaagcgcagg
      781 gtgagatcaa cgcgtcgctc cgggtagcta ttaatcaggc ggatgcgaag atcaaacagg
      841 cggacgcaag gatcaccaac acggatgcga ggctcgaccg gctggaggcg ttaatcgaac
      901 gctacatcga agggcgcaac gggcagggtt agcgtgccgg ttcaagctgt cgtttcccac
      961 ctcatttgag aacaatttcc cgcccggtcg cccctcaagt tcaatcattc gacttctttc
     1021 cttcgagcct gatcaggcga ttcgtctccg acaggaagtt ttcactaaag gcgagtttgc
     1081 cgtcgctgac gaggcgacgc ccgcacgcga tcacgtcctt gatttcgagc gagtctttgc
     1141 gcagcacgag caggtcggcg tcgcggcctg tttcgagtcg ccctttttgt ttgagttgga
     1201 ggacgctcgc cgggttgatg gtgacgtgcg cgagcatctg tgcgagcggg aagttgtgtt
     1261 cgaggacgca ggcgcgaatt tgattgagga gactccgggg gctgttgatc gaagcgtcgg
     1321 aggagacggt gaggcgcgcc gcgtcgccgc cattgtcgag atagaaacgg agccacttcg
     1381 gcaggtcttg ttcgaccgtg tcgagatcga cgtaagagcc gcgcgtggcg agttcgatgg
     1441 cctctcgcat caacgcttcg tcgcgctcaa cgtgcgtcgg gtagagaagc gccgggttga
     1501 tctcgtattc atcgatcaag gcgcgcagca acttgaggcg cgacttgcct tctcccacat
     1561 gaaagtgcgt gacgccgcac ttgcggctga gcaggccgcc cacataagca tctttgacga
     1621 ctttggcgag ttcgggcgca tcgtgtgcgt tggcgcgttc gtctgcgatg gcgacctcgc
     1681 ccgcgccgat aacttccgca atgcagatta tatcttcgcg gacgccctgc atcagcgtgg
     1741 tcggcggcac gttgtagccg cccgtccaga cgtaggccgt gaggttctcc tcgttcaagc
     1801 ctttggcttt cgcgagcagc ccggtcatcg tcttcgtcgt cgtgtctacg ccgaggcagc
     1861 cgacgacggt ggtgattccg gcgagggcga gttcgctcgc ggagacttcg ggagtctgcg
     1921 tgctgaaccc cttttcgccg ctgccgccga gcagatgttg gtgcgggtcg ataaggccgg
     1981 gcgtgacgag acaatcgctc gcttcgacaa cttcgagttc gagcttgagc ggcgcgagcg
     2041 tcgcggcatc gatctggccg atggccgcga tgcggcggtc gaaaagcaga acgtcctgtc
     2101 gtccgagcga cgcgggcgcg tagacttcgc cgttcttgat gagcgtgagc atccccggct
     2161 ctcagttcgt cgccacgctc agttgcggca gcgcgaactc tccgatgctc gtcaccggca
     2221 cggctccgtg ttgttcgagc acttcgagga cgggttcgag acgatcatag aagatgacga
     2281 gcacgtcgcc ctccttcatc tcgttcaatt cgcgcgagaa ggcagcgatt tcatcgagca
     2341 cgacgcggca ttcacgatcc ggcgatgcct cgttgacggc gcggcagagc acgcccgcca
     2401 cctcgccctt gcgcctcccg cgcaggtctt tatcttcctt gataatgatg cggtgaaaac
     2461 ctctcgccgc cacgcgtcca gccgtttcaa tcaaggcatc gtcgcggtcg ccgggaacgc
     2521 cgaggatagc cgtcacgtgc cgcccagacc agagcgcggc catgcgacag acggcctcga
     2581 aggcatccga gttgtgcccg tagtcaacca tcacgtagcc ctcgccgacc tgatagagat
     2641 tcgcgcgtcc cggattatgc tgatcgttgc ggaaggtctt gagcgcggcg gcgacgttct
     2701 cgcgcgtcac gccataggcg cggcaggccg caatggccgc catcgcgttc gccacctgaa
     2761 aacgcgccgt cccgctcatc gtcaccggaa tcgcggagac ttcgacgacg cggtgctcct
     2821 cgcgccccac ggcttccatg atccagccat cgcgcacgaa gaacgcgccg ccgccgatgc
     2881 cgagatattt tctgatcagc aggttgttat cgttgagcga gaagtagatg aactgtttat
     2941 ccgtgcggcg cacgcgcggg ttctcgatga gttgcacgag ttgctcgtca tcggcgttga
     3001 ggatgagcgt gccgccctcg cgcacgcgct ctgcgatgag cgatttgatg tgcaccaaat
     3061 cttcgatgct gcggatgccg tcctgcccga tgtggtcttc gcggatgttc gtcatcacgg
     3121 agatgtccga ccaatcatag ccgaggccgc gccgcgcgat gccgccgcgc gccgtctcca
     3181 gcacggcgac ctccacggtc gggtcggaga gcacgacgcg cgccgaatga gggccggtcg
     3241 tgtcgccgcg tgagacgaca tgaccgtcga tatgtatgcc gtcggtcgtc gtcatcccca
     3301 cgcaccagcg catctcctga agcacgtggc cgatcatgcg cgtgacggtc gttttgccgt
     3361 tcgtgcccgt gatggaaatg atcgggatgc gcgcctgcgt atcatgcggg aagagcatgt
     3421 cgaggatcgc gccgcccacg tcgcgcggtt ggccgtgcgt cggcgcggtg tgcatgcgta
     3481 agcccggcgc ggcgttggct tcgatgaagc cgtcgccctt ctgcaaaggc tgtgagatgt
     3541 ctgcggcgac gagatcgagg ccgcagatgt ccagaccgac gatgcgtgcg gcgcgctcgc
     3601 aggctcttct ggtttcaggg tggacgaggt cagttacatc agtggccgtg ccgccggtcg
     3661 agaggttggc ggtttcgcgc aataagacaa gctcgcccgc cgccggaatt gactcaaggc
     3721 tcaggccgcg tttgagcagg ctgctgagca tcacctcgtc cactttgatc tgggtcagca
     3781 ccttgtcgtg cccctcgccg cgcagggggt cttggttaac aagttcgatc aacccggcga
     3841 gcgtatgttc cccgtcgccc acgacgtgac agggcaggcg ttcgctggcg gcgacgagtt
     3901 ttccgttcac cacgagcacg cggtaatcgc gcccgacgag ttgctcctcg atgagcacgt
     3961 catgcgaata ggcttgcgcg atcttgaagg cttcaaatac ctcgatgggg gtggtgagat
     4021 ttagagagac gccttttccc tgatggccgt ccatcggctt gacgacgacg ggcgcggcga
     4081 aatccgacag gacggcgatg gcttcgtccg ccgtctccac gacgcgcccg cgcggcacgg
     4141 gcaggtctgc ctgttcgagg atcgtcttgg tcaactcctt gtcgcccgcg atctcgacgg
     4201 cgatgccgcc cgttgcgctc gtcatcgcgg cctgaatgaa cttgcgattt ttgccgtagc
     4261 cgagttggac gaggctatcg ctgttgacgc gaaaccaggg gatgtcacgg cggcgcgcgg
     4321 catcgataat ggcgcgcgtg ctcggaccga gttccgtgcg cgcgatgatc cggcgcgctt
     4381 cttcgagttt ctctgcgagc gggtaggagt cgcctttgac gagcgcgtca acgagttcga
     4441 ccgctacttc taacaggtaa tgcatcccgt gctcggcctt gtaggcgacg atcacgttgt
     4501 agcagccggg ttcgtccgca tagatggtct tcccgtaata gacgggcacg cctgccatgc
     4561 ctgaaagttc gagcgcgacg tgttcgacga tgtggccgaa gtacgtcccc gtcgagagcc
     4621 gttcgacgaa gccgcccgcc cgccctttgt tgcaatggtg ctcgtcgagt cccggcagga
     4681 gggagatgag gcgttcgttg aaaccggaaa tctcaaggct ttccgtctcg gccagttcct
     4741 ccagatagag tttcattacc agcacaggct tatggttgta aatgttcgcc ccggcgagcg
     4801 ttcttatttt gtccaccctc atgttcgtat cctctcggtt aaattcagtc gccgcaacgg
     4861 tgcttcagga cagactttca ggagacacgt atccgacagt tcgcgccggg cgcggtctgt
     4921 tcacaggcaa cgtcttgacc gatcattcaa caggtaatct ggcggattat aggagttggc
     4981 agagggtggg tctgtacgac aacggactaa taagctctcc gtttcgcaca gattttgtct
     5041 ggggttttcg accggatttt gtttagagtt ttccgctcaa gccttctccg gcagccagat
     5101 gtcaacggcc agagattcgg cgtagtggac gagcggcttg cctttcagct tcggcaaata
     5161 atcggactcc acgatgtctg actcgtgcga gatgagcttc gcctgccgga gcggccacgg
     5221 cctgtggttg atgcgcgcgc ggtagatgct ctcgccggaa gcggagtaga ggcagtaacg
     5281 ctctgtgagg aagaattcca gcgtgcccgg ttctgactcc ggcaactcgc gtccgatctc
     5341 ccaggacgct ttgaaacggg cggggcggtc ggcgtccgtg cgctcaaggt cgtaatgaat
     5401 cgtgtcgcct tcttgtcgca gatcaatgcg tgcgttgaag tagggcagga agtagaaggt
     5461 gcgcgcgccg aagacggtca tcgaactgtt ggtgttgagc gaaaagaacc agacgccggg
     5521 cacgcccctg tagagcacgt aggtgcgcac gttcaactcg tggaagtcgc tgagatacgg
     5581 gacgggcggc gtaaagctga gccgcacgtc ccacagcgta aacggcgtca cgccgatcca
     5641 cgcctcgccg tcgaacgtgt caatcgtgag ttgcggcggg atgagcgaac gcagacgctc
     5701 cggcgagatc ggccagtgca tgaagagcaa cttgccccag tggtgctcca tcaaaggcaa
     5761 ctcttcggga cgctgccgga tcgtctcgcg gtcaacgccg tcgtcgatca ccttcttgcg
     5821 cccgacggcg cgcgtcgtgt tggaagtttt actgagcgcg gtcttgctgt cggcgtccgt
     5881 cttgcgggtc gccttcttct tcgtgcccgc tctcttcttt gcgccgcccg tcgcccgcgc
     5941 gccgttgttg ccgccgtccg ttagctcgtc gtccgctgtc ttctcttttg tcatcgcttc
     6001 atcgccccgc ccgtcgagtg tccgacgcaa actatttctt cccgcgtttt tgatcgccct
     6061 tgtcgccctg ccccggcttg accgtcgttt tcgctttacg cttcacgtcc tgcgagagcg
     6121 agcggcctac gtcgtccgac tcgctgaaac gtccctcgtc gtctcgccgc acataacgct
     6181 tatcggttcc cgtgtcgatc aactcgcgtt tgcctgatga tttcttagtc gccatgaaac
     6241 ttccctcccc ttcttcgttg gaaaattatg cgccggaatt tttcacgccc cggtgcgcgt
     6301 ggactttcgc ttccgtttgc cggacatgct ttcgagttcg agcaacgctt cgtctaacgt
     6361 ctgcctgccg ttgagcacag gcttgaacag gtcgcccgtg cgcgcgaggc gcgaagagat
     6421 gttcttgatg ttgaagtcct gcgggtgaat ctgctgtcgt tcgacctctt cccaactgag
     6481 cggcgcggag acgggcgcgc cgggacgcgg gcgcaccgag tagggcgcga cgaccgattt
     6541 gccgcgcgcg ttctgcatgt gatcgacgta gatgcgcgcg tgctggcgtt tctttagaga
     6601 gcgttcgaca gtggcgaccg cagagttttc gcgcacgacg agttttgcca cgagttcggc
     6661 aaagcgcgcc gccgccgcgt agtcgtgcac ggccttgatg gggacgtaga tgtgcagccc
     6721 gcgcgagccg gaagtcttcg catatgactc aagcccgagg ccattgagaa tatccctcgt
     6781 gctcacggct gtctcgcaga cgatcttgaa atccacgttc tcaggatcga gatcgaagac
     6841 gacccaatcg gggcgatcca gttgctcgat gcgcgaatgc cacgggtgac gctcaatcgc
     6901 ggcgaggttc gccatataga cgagcgtggg gcgttcgccg ccgacgatgt aatcgaccgc
     6961 gtgcccttcc tcgacatcga tctccgccgt cgcgacaaac tccggcgctt cgttcacgtc
     7021 atgctggtgg aaagagccgc cgccgatgcc tgccgggtaa cgcttcatga tgagcgggcg
     7081 gcctgcgagg tagggcgaaa gatagtgcgc gatctcgtag tagtacctga cgagatcgcc
     7141 cttcgtataa ccgtcttccg gccagtagac tttatcgaga ttcgtcaaag ccacgacggc
     7201 ctcgccgatc ttcacgttga cgttgccgct cagtctcttc cgcgcgaaaa atctttgcgc
     7261 ccccgtcgtc tccacatcct tcggcggctc gacgcgcttg acggccttgt gttcggctac
     7321 gacggcggcg gcgcgcttct gtctcttcgc tccctgttca cgctgccatg ccgcgccgcg
     7381 ctcatcatcc ggctggcgca gttgcaactg ccagcccgtc tccgcgaatt catcgctgct
     7441 cttgatgagc aaccattgcc ccttccaatg ccccatccgc acgaagctga attcgccgcg
     7501 caacttctct ccatgcaggc gaaacttcaa acgcccggct tcatactgtg cgagcgcgtc
     7561 cccgtcgccc acgagttcat aagttccccc gtcccatctc atgtgctcgc ccgcgccgta
     7621 gttgccttcg ggaatgtgcc cctcgaattt cagatactcg acgggatggt cttcggtcgg
     7681 cacggcgagc cgcttctccg ccgggtcgag cgaaggacca cgcggaattg accacgactt
     7741 gagcacgccg cccatctcca gtcgaaaatc gaagtgtaac ttctccgcgt gatgctcctg
     7801 cacgacgaag cggcgcaggt ttcgcttcga caccgtcccc ttcggttcag gcgtccgccg
     7861 gaagtcgcgc ttccgttgat aaacttccag ccccattttt ttcacccgct caggttttcg
     7921 cgctcacgcc cgcgcgcaat ttcaacccga ctctgctaga ttctaatgtc atcaggacgc
     7981 ttttgaatgt acgctattct acacgacgct ctccgtaaac ggagacacat atttcccgga
     8041 gccacttttc gcacaagggt gaaaacagtt gggacacgca atacttctcg gcgactcggt
     8101 ttttgacaac gcggcctacg tcggcgcggg cgacccggac gtggtgcgcc agcttcagga
     8161 acaactgccg cacggctggc aggccaccct gctcgcggtt gacggcagcg tgactcgcaa
     8221 catccgcgca caggcggagc ggcttcccac cggctcaagc catctcatct tgagcgtcgg
     8281 cggcaacgat gcgctcggct acgcacgcct gctcgaagca cccgcgcgct caatggccga
     8341 agctctcacc atgctcgcac gcgccggaga cgacttcgaa cgcgaatatc aactcatgct
     8401 cgaagagctt ctcagccgcc gcctgcccac cgccgtctgc actatctaca acccgcgctt
     8461 caccgaagca cagaggcaaa agctggccgt cacagccctc tgcatcttca acgacgcgat
     8521 catccgcgcc gcgttcgcgc gcggcctccc gctcatagac ctccgcttgg tctgcaatga
     8581 cgaccgcgac tacgccaatc ccatcgaacc gtcggcgcgc ggcggcgcga agatcgccgc
     8641 cgccatcaaa cgcgtcgtca ccgaacacga cttctcgcgc gggcgcacgg aggggttcgt
     8701 ctaaagcgat ccgttttgcg tcccggttcc tgcgcttgac ctgcaacgcg ccgcgtccct
     8761 acaatccacg cgcgttcccc aaacgccccc attcataatc cggctaaatt ttcagaccga
     8821 gggagattac tatgacaacc aaacctaaca cgggcacgca ggacacgacc tacaacttgc
     8881 tgagcatcat ctatcatgcg ctgcaaggcg cggagactta cgagaaatac atcaacgacg
     8941 ccgaacaaca cggcgaccgc gaactcgcgc agtacttccg cgacgtgcgc gaccagaaca
     9001 tcaaacgcgc cgaacaaggc aaggaactgc tctccgcgcg tctcggccag gatcaggcgc
     9061 aaggccaatc cgcctagacg cctccggtag cagcgatctc aaacacgcac gaggccgccc
     9121 gcacgagcaa gagactttcg atgtcgaaaa tctcttgccc gcgcgggcgg cctcgtgcat
     9181 tcacactctt agagcgtttg ccctcgcgcc tgctcagtac ttcttcacgt tcgggtcaac
     9241 gtcgttcgac caggccgtga tgccgccttt gagattcagc agacgcccgc cgaagcccgc
     9301 gcctcgtaac gcctcgatgg ccttcgcgct gcgcccgccc attttgcaat gcacgaccgt
     9361 ctcgcggctc tcgtcgatct cgcctttgcg tgtcacgatc tgtccgagcg gaatcaattt
     9421 cgatccttcg agccgcgccg cttcgtattc gttcggttcg cggacatcta tgatctgcaa
     9481 gtcgtcgccc ctgtccagac gctctttcag ttccgtcgca gtgatctctt ccatccttag
     9541 tttgtcctcc tccctcacaa ttcaaagcaa actccggatt gcttacgact tctccagcgt
     9601 gcgcttggcg aaataccagt ccgtcacgtc gtactcggat tgcagacgct tctctgcgtc
     9661 ggcgatggtt tgcggcggcg gcacgatcac ttgatcgccc ggtcgccagt tggcgggcgt
     9721 ggcgcaggcg ttggcatccg cagtctggag tccgtcgacg gcgcgcagga tttcgtctat
     9781 gttgcgcccc agagacatcg ggtaatagat gagcgcgcga atg
//
LOCUS       DASWNS010000212         6425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_113165, whole genome shotgun sequence.
ACCESSION   DASWNS010000212 DASWNS010000000
VERSION     DASWNS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113165"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VGB76_07225"
     CDS             complement(<1..503)
                     /locus_tag="VGB76_07225"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEY0098731.1"
                     /translation="MEELPVTEQENPRTTQISSLSGAEIVRLMNEEDALVAEAVGRTL
                     AEVARAVEGIVERLRDEGGRLFYVGTGTSGRLGVLDAAECPPTFGVSPELVQGIIAGG
                     YEACYRAVEASEDDRGAGVRDLVARGVTGRDAVVGIAASGRTPYTVAALEHARALGAF
                     TAAVTCAP"
     gene            complement(589..1584)
                     /locus_tag="VGB76_07230"
     CDS             complement(589..1584)
                     /locus_tag="VGB76_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY0098732.1"
                     /translation="MKARRETTNFSHAPRDLNAPLIVLGVDGGGTKTQAIITDEEGHT
                     LGEGLAGPSNPLRVGVSNAAAAVREAVDRACAAAGVRRTDIASAEVGLAGVRRADLKE
                     RMTDALHVLGINSVEVVTDADIALYGATNGKPGLVLIAGTGSICCGKNARRKHICAGG
                     WGPLAGDEGSGSWIARRALQAVAQATDGRGAATSLDEAACRYFNVASPEDLSLAIYAP
                     SMTNERIAGFARRVIDAAANEGDVVAVEIVADAGRELASLANSVIRKLRIEREPFQVA
                     YVGGVFSAGELVLDPLRRAIKLSAPQAFLAPPQLSPAVAAATMAREHVRRLALAG"
     gene            1742..2710
                     /locus_tag="VGB76_07235"
     CDS             1742..2710
                     /locus_tag="VGB76_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0098733.1"
                     /translation="MEIRQLRALAAIAETHTFTAAAARVHVTQAAISMQIRQLELETG
                     ALLFVRTPRRCVLTHAGERLLERARRILREHDAALAELAELSGTSHGRLRLGSASAMF
                     SSDQLPQILKDLRARCEHVEVTVTSGTSESLVKQIMAGELDIAFISLPVDEARGVQTE
                     LLLKDELIAIANPAHPLAHKRVISAFELAGQKLILGERGGNTRRLIDEFFAEAGLRPV
                     VAMELSRLTSIKRMVESGMGVGIVPVRSALEEIAEGRLVRWWIEGAQINSELGFAYLT
                     GGYQSPVTQTFISLCRENLAAASEANKPRKQRTRPARKLKAETKRR"
     gene            complement(2803..3183)
                     /locus_tag="VGB76_07240"
     CDS             complement(2803..3183)
                     /locus_tag="VGB76_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098734.1"
                     /translation="MTGARTEILYVEDTPDTRFLVEWSLKQEGFSVTAVQTAEEGLRL
                     AREHSYALILLDIGLTDMDGLALCREIREFNHTTPILFFTAYAPLLDHQEAIRAGAQG
                     CLRKPEDTARLVPAIRTLLGQSDA"
     gene            complement(3320..3910)
                     /locus_tag="VGB76_07245"
     CDS             complement(3320..3910)
                     /locus_tag="VGB76_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY0098735.1"
                     /translation="MWTFPFSRCDKLTNTGDTTRVEKRAARVKLLLMDCDGVLTDGRI
                     TLLENGDEEKSFHTRDGHGLVLLHRAGLQSGIISGRTSTLVERRARDLGIAYVRQGTW
                     DKIKDFGEVLAAAKVEESDVAYVGDDVTDIPLMMRCGFAVAVADATAETRAAAHYTTS
                     LPGGFGAIREVCELILKAQGRWPELMRRYTEATIKQ"
     gene            complement(3879..6188)
                     /locus_tag="VGB76_07250"
     CDS             complement(3879..6188)
                     /locus_tag="VGB76_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098736.1"
                     /translation="MNISSLRKLTLVIFTLLSASNVARAQDAATPVWRVSSFDVTANL
                     PAANAAERAINARSVVSVRNVGQGSSGTFTLRINPAAEIKSVTVGDASARFNTRPEPR
                     TELQMVIVTLPAPATPGTTLTVALDYRLPVAANTGLAALSPEGAQFLPLSGWYPSPNS
                     QFSPRGADTAPLRLTVNAAGGEMVVASGRATGASFEQKLSAQPFFLTGRWETIEGAAD
                     ARGVSALIPRGATPDERKRAETLVALAASARSFYTGLFGALPETPIRLVGVTRGGGFD
                     LGGTVLLDNAVFRRSKTDAITAQLIGEAVARMWVGGATPVRNEGSGVVREGLARFLAT
                     LFLEKEFGREAADAERLRQRIAYSAVARRDAPLSVTTPLEPTYYLSVANKGAMIWRLA
                     DRALGREAFLAVLREQLQKGRESEITLAGLRVALASGGGAALKSALDAGFDQPTEIDL
                     LIGLPQQRGGEWVSALRNTGAIETIVSAAAFTESGERVLSQTTIPARDFADVHFKTPA
                     RIVRVEVDPEKIYPQLDYSNDVAPRAPALEESLEEATRLLAAGQHAQSEAIARALVTR
                     APLMQDARILLARSLLEQNKLADAEREFRAALDERLPLPVTLAWANIGLGEIASRRNQ
                     AAEAAKRFDEAVRVEGGYAPTLAARAARLKAEAASGASAPAIDESVRAAVTQFDQTIR
                     GGRKAELDAFIMPGELANFAKGIIGSQPELWQTKLLRTESLGSDRFAADVNITAKSLG
                     REQAGTAVLVFARTGNTMRLVDIPIFEVR"
     gene            complement(6207..>6425)
                     /locus_tag="VGB76_07255"
     CDS             complement(6207..>6425)
                     /locus_tag="VGB76_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098737.1"
                     /translation="GHATGGQSEYIEPLARAGVACGVDAVFMEVHEEPSRALSDGPNA
                     LPLARLEPLLYMLREIHELVRRQAAMEN"
BASE COUNT         1120 a         2080 c         2053 g         1172 t
ORIGIN      
        1 ggcgcgcacg tgacggcagc cgtaaacgcg ccgagcgcac gcgcatgttc caacgccgcc
       61 acagtgtaag gcgtgcgtcc cgaagcggcg atgccgacga cggcatctct gcccgtgacg
      121 ccgcgcgcaa cgagatcgcg cacgcccgcc ccgcgatcat cctcggaggc ttcgaccgcg
      181 cgatagcaag cctcgtagcc gcccgcgatg attccctgca ccaactccgg cgacacaccg
      241 aacgtcggcg gacattccgc cgcatcgagc acgccgagcc gcccggacgt gcccgtgccc
      301 acgtagaaga gtcgcccgcc ctcgtcgcgc agacgctcga cgatgccttc aaccgcacgc
      361 gccacttccg ccagagtgcg ccccaccgct tccgccacga gcgcatcctc ctcgttcatc
      421 agacgcacta tctctgcgcc ggacaacgaa ctgatctgcg tcgtgcgcgg gttctcctgt
      481 tcggtgacgg gaagttcttc catgagtctc aaggctcaag tcccggttta caggtacgga
      541 gtctcaatgt ctgtagagct ttcagacctg agactccgga cttgggactt atcctgcgag
      601 cgcgagtcgc cggacgtgtt cgcgcgccat cgtggcggcg gcgacggcgg gcgagagttg
      661 cggaggcgcg agaaaggctt gcggcgcgct gagtttgatc gcgcggcgca aaggatcgag
      721 gacgagttcg cccgcgctga acacgccgcc cacgtaggcg acctgaaaag gctcgcgctc
      781 gattcgcagt tttcggatca cgctgtttgc gagcgaagcc aattcgcgcc ccgcatcggc
      841 gacgatctca actgccacca cgtcgccctc gttcgccgct gcgtcgatga cgcggcgcgc
      901 aaatcccgcg atgcgctcgt tggtcatgct cggcgcatag atcgccagcg agaggtcttc
      961 cggactcgcc acgttgaaat aacggcacgc ggcttcgtcc agactcgtcg ccgcgccgcg
     1021 cccgtccgtg gcctgcgcca cggcctgcaa cgcgcgccgc gcgatccagg aaccgctccc
     1081 ctcatcgccc gccagcggcc cccagcctcc ggcgcagata tgtttccggc gcgcgttctt
     1141 cccgcagcag atcgaacccg tgcccgcaat caacaccaga ccgggcttcc cgttcgtcgc
     1201 gccgtagagc gcgatgtcgg catccgtcac aacttcaacc gagttgatgc cgaggacgtg
     1261 caaggcgtcg gtcatgcgct ccttcaaatc tgcgcgacgc acacccgcca gtcccacctc
     1321 cgcggaggca atgtccgtgc ggcgcacgcc cgccgccgcg cacgcgcggt ctaccgcctc
     1381 gcgcaccgcc gccgccgcgt tgctcacgcc gacgcgcaaa ggattggaag gccccgcgag
     1441 tccttcgccg agcgtgtgtc cctcttcatc cgtgatgatg gcttgagttt tagtgccgcc
     1501 gccgtccacg ccgagcacga tgagcggggc gttcaaatct ctgggcgcat gggagaagtt
     1561 tgtcgtttcg cgtctggcct tcatcgtttg tccgggctga aagcatcggg ggagacgcct
     1621 ctagcgataa ccccttagta tggcggctaa gatgggttga ttgtaaaaag gattgacata
     1681 taatggaaac taaaagattt tattcgcttg ataattattc ctgatagaaa ggttttgctc
     1741 gatggagata cgacaactca gggctttggc cgccatcgcc gagacgcaca cgttcaccgc
     1801 cgcggcggcg cgcgttcacg tgacgcaagc cgcgatcagc atgcagattc gccaactcga
     1861 actggagacg ggcgcactcc tcttcgtgcg caccccgcgc cgctgcgtgc tgacgcacgc
     1921 cggggaacgc ctgctcgaac gcgcccgtcg catcctgcgc gaacacgatg ccgcgctcgc
     1981 agagttggcc gagttgtctg gcacatcgca cgggaggctg cggctgggga gcgcgtcggc
     2041 gatgttcagt tcggatcagt tgccgcaaat tttgaaagac ctgcgtgcgc gctgtgaaca
     2101 cgtcgaggtt acggtcacgt cgggcacgag cgaatcgctc gtcaaacaaa ttatggcggg
     2161 cgagttggac atcgctttca tctcgcttcc ggttgacgaa gcgcgcggcg tgcagacgga
     2221 gttgctgttg aaagacgagt tgatcgctat cgccaatccc gcgcacccgc tcgcgcacaa
     2281 gcgcgtcatc agcgcgttcg agttggcggg gcaaaaactt atcctcggcg agcgcggcgg
     2341 caacacgcgc cgtctgattg acgagttttt cgcggaggcg ggtttgcgcc cggtggtggc
     2401 gatggagttg agccgcctga cttcgatcaa gcgcatggtg gaatcgggga tgggcgtcgg
     2461 catcgtgccc gtcaggtcgg cgttggaaga gatcgcggaa gggcgtctcg tgcgctggtg
     2521 gatcgagggc gcgcagatca actcggaact cggtttcgcg tacctcacgg gcggctacca
     2581 atctcccgtc acacaaactt tcatcagctt atgtcgtgag aatctcgccg ccgcctcgga
     2641 agccaacaag ccacgcaaac aacgcacgcg cccggcacga aagctcaaag cggagacgaa
     2701 gcgacgctga ctttaagctg tccggttcgg cctctcagcg tcgaatcttt ctattgcgtc
     2761 cggtggcaag acgccctcgg agttaagtgc gccagggcaa gcctatgcgt cggactgccc
     2821 gaggagcgtg cggatggcgg ggacgaggcg ggcagtgtct tccggtttgc gcaggcagcc
     2881 ttgcgctccg gctctgatgg cttcctgatg gtcgaggagc ggcgcgtaag cggtgaagaa
     2941 gaggatcggc gtggtgtggt tgaactcgcg aatctcgcgg cagagcgcga ggccgtccat
     3001 gtcggtcagg ccgatgtcga ggaggatcag cgcgtaggaa tgttcacgcg cgaggcgtaa
     3061 gccctcttcc gcagtctgca cggcggtcac gctaaagcct tcctgcttca aactccactc
     3121 caccagaaaa cgcgtgtccg gcgtgtcctc aacatacaaa atttccgttc gcgcacctgt
     3181 catagttttt cactccgtga aaaaagtgac gagtgacggg tgacaagttg aaaagccgtg
     3241 aatcgtcaat cgtcaatctt caatcgtgaa tagtagaaga ttcacttgcc ttctcttgtc
     3301 actcgtcacc tgtcactcgt cactgcttta ttgtcgcttc cgtatatcgt cgcataagtt
     3361 caggccagcg gccttgtgct ttgagaatca attcgcagac ttcgcggatt gcgccgaagc
     3421 ctccggggag actcgttgtg tagtgtgcgg cggcgcgcgt ctctgcggtc gcatcggcga
     3481 cggcgacggc gaacccgcag cgcatcatca gaggaatgtc cgtcacgtcg tcgccgacgt
     3541 atgccacgtc gctctcttcg accttcgcag cggccagcac ttccccgaaa tccttgatct
     3601 tgtcccacgt tccctgccgc acgtaggcga tgccgagatc gcgcgcgcga cgctcgacaa
     3661 gcgttgacgt gcggcctgag atgatgcccg attgcaaccc ggcgcgatgc agcaggacga
     3721 ggccgtggcc gtcgcgcgtg tgaaaactct tctcctcgtc gccgttttcc agaagcgtga
     3781 tgcgcccgtc cgtgagcacg ccgtcgcagt ccatcaaaag cagtttaacg cgcgccgctc
     3841 tcttttcgac tctcgttgta tcgcccgtgt tcgtcaattt atcgcacctc gaaaatggga
     3901 atgtccacaa gccgcatcgt gttgcccgtg cgcgcgaaga cgagcaccgc cgtgcccgcc
     3961 tgttcgcgtc cgagcgactt ggccgtgatg ttcacgtccg ccgcgaaacg atccgagccg
     4021 agcgattcgg tgcggagcaa cttcgtctgc cagagttcgg gctggctgcc gatgatgcct
     4081 ttggcaaagt tggcgagttc gccgggcatg atgaaggcgt cgagttcggc tttccgcccg
     4141 ccacgaattg tctgatcaaa ctgcgtgacg gcggcgcgca cgctctcgtc tatggcgggc
     4201 gcggacgcac ccgacgccgc ttccgccttc aaacgcgctg cacgcgcggc gagcgtcggc
     4261 gcgtacccgc cttcgacccg caccgcttcg tcaaaacgct tcgcggcctc cgccgcctga
     4321 ttgcggcgcg aggcgatctc gcccagcccg atgttcgccc acgcgagcgt cacgggtagc
     4381 gggaggcgtt cgtcgagcgc ggcgcgaaac tcgcgttcgg catcggctaa tttgttctgt
     4441 tcgaggaggg agcgcgcgag caggatgcgc gcgtcctgca tgagcggcgc gcgtgtgacg
     4501 agcgcgcggg cgatggcttc ggactgcgcg tgttgccctg cggcgagcag gcgcgtggcc
     4561 tcttcgagcg actcttcgag cgcgggggcg cgcggcgcga catcgttcga gtaatcgagt
     4621 tgcgggtata tcttctccgg atcaacttcg acgcgaacga tgcgcgcggg agttttgaaa
     4681 tgcacgtcgg cgaaatcgcg cgccgggatc gtggtttgcg acaggacgcg ttcgccgctc
     4741 tccgtgaagg cggcggcgga aacaattgtc tcaatcgcgc ccgtgttcct gagcgcggac
     4801 acccattcgc cgccgcgctg ttgcggcaag ccgatcaaga ggtcgatttc ggtcggttga
     4861 tcaaacccgg catcgagcgc gctcttcaag gccgcgccgc cgccggaggc cagagccacg
     4921 cgcagcccgg ccagcgtgat ctcgctttcg cgtccctttt gcagttgctc gcgcaggacg
     4981 gcgagaaagg cttcgcgtcc gagcgcgcgg tctgcgagtc gccagatcat cgcgcctttg
     5041 ttggcgacgg agagatagta agtcggctct aaaggcgtgg tgacggagag cggggcgtcg
     5101 cgtcgggcga cggcggaata agcgatgcgc tggcgcaggc gttcggcatc ggcggcctcg
     5161 cgcccgaact ctttttccag aaagagcgtc gccagaaaac gcgccagccc ctcgcgcacg
     5221 acgcccgaac cttcgttgcg cacaggcgtc gcgccgccga cccacatccg cgcgacggct
     5281 tcgccgatga gttgcgccgt gatggcgtcg gtcttgctgc gacgaaagac cgcgttgtcg
     5341 agcaagaccg tgccgccgag atcgaagccc ccgccgcgcg tgacgccgac gaggcggatc
     5401 ggcgtctccg gcagcgcgcc gaacagtccc gtataaaaac tgcgcgcgga cgcggccaat
     5461 gccacgagcg tttcggcgcg cttgcgttca tcaggcgtcg cgccgcgagg aatcaacgcg
     5521 ctcacgccgc gcgcgtcggc agcgccttca atcgtctccc acctgcccgt cagaaaaaac
     5581 ggctgcgccg aaagtttctg ttcaaaactc gcgcccgtcg cccgtcccga tgcgacgacc
     5641 atctcgccgc ccgccgcgtt gaccgtgaga cgaagcggcg cggtgtcagc cccgcgcggg
     5701 gagaactgcg aattgggcga aggataccag ccagagagcg gcaaaaactg cgcgccttcg
     5761 ggcgacaagg cggccagccc cgtgttcgcc gcgacgggca ggcgatagtc gagcgcgacc
     5821 gtgagcgtcg tgcccggcgt ggcgggcgcg ggcagcgtca cgatcaccat ctgcaactct
     5881 gtgcgcggtt ccgggcgcgt gttgaagcgc gcgctcgcat cgccgacggt caccgatttg
     5941 atctctgccg ccggattgat gcggagcgtg aacgtgccgc tcgaaccctg cccgacgttc
     6001 ctgacggaga cgacggagcg cgcgttgatg gcgcgttcgg cggcgttggc ggcgggcagg
     6061 ttcgccgtca cgtcgaaact cgaaacgcgc cacacgggcg tcgcggcgtc ctgcgcgcgc
     6121 gccacgttcg aggcagacaa gagcgtgaaa atgacgagag ttagtttgcg aaggctggag
     6181 atgttcatac ttgagcgagc gtctgttcag ttttccattg cggcctgccg ccgtacgagt
     6241 tcgtgtatct cgcgcagcat gtagagcagc ggttcgaggc gtgcgagcgg gagcgcgttc
     6301 ggcccgtcgg agagcgcgcg cgaaggctcc tcgtgcactt ccataaagac cgcgtccacg
     6361 ccgcaagcca ccccggcgcg ggcgagcggt tcgatgtact ccgactgccc gcccgttgca
     6421 tgacc
//
LOCUS       DASWNS010000213         4313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_424180, whole genome shotgun sequence.
ACCESSION   DASWNS010000213 DASWNS010000000
VERSION     DASWNS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424180"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VGB76_07260"
     CDS             complement(<1..458)
                     /locus_tag="VGB76_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY0098738.1"
                     /translation="MRIRLIWVGKTKNEHVRALVDDYLQRLGRFVRCEVTELRESASG
                     DERAVIEEESKRIAGALGAGGALTVLLDVEGAREWSSPELAAQVERWQLDGRKEIAFV
                     IGGHLGVAPELKAKAQVRWSMSRLTLTHEMARVVLVEQLYRAYTIMHGLP"
     gene            complement(537..1472)
                     /locus_tag="VGB76_07265"
     CDS             complement(537..1472)
                     /locus_tag="VGB76_07265"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0098739.1"
                     /translation="MIELVANSEAMREAVRLASRVATTDANVLVTGESGAGKDALALH
                     IHRQSKRAAASFVKIDCAALPAELLEAELFGYERGAFTGATESKPGRLEAAHRGTLVL
                     DEIAHLSTDAQAKLLRVIEEREFERLGGRRAVRVDARLIALTNVDLDEAVRRRAFRPD
                     LFYRLNVVRIHVPPLRERREDVRKLARGFLKTYASKHGRRAAQIEPDALLLLETYDYP
                     GNVRELAHVLERAVIVADGDRVTSAHLPEAVRAAASLQRRRVERMTLAELEAAYIAET
                     LASTRGNKSEAARLLGISRKNLYERLARQKGNDEG"
     gene            complement(1469..2050)
                     /gene="rsfS"
                     /locus_tag="VGB76_07270"
     CDS             complement(1469..2050)
                     /gene="rsfS"
                     /locus_tag="VGB76_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY0098740.1"
                     /translation="MKQNETTPEESALTTKKRATLKKEPDTADAAATAAATASAHGDE
                     SGADAAATPQQEEGLDARLESALRAASDKKALDLVVLDLRPVATFTDYFVIASGTNAR
                     QVQAIADEITERLKREGTRAERIEGYRTAEWVLVDYGDFIVHIFEEKARRFYDLERLW
                     REGVRVPLPADLQAETRGSSSSGASSDKLSNES"
     gene            complement(2004..2738)
                     /gene="nadD"
                     /locus_tag="VGB76_07275"
     CDS             complement(2004..2738)
                     /gene="nadD"
                     /locus_tag="VGB76_07275"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY0098741.1"
                     /translation="MNWPRHSRIALYGGTFDPVHNGHLAVARELLKLFSLDEVLFIPA
                     HVAPHKRNVPPASAYHRYAMLALATRDEPRFRISTVELDEPRKPYTIETILRLRDAPG
                     GQLLFVMGADSWEEITTWREWERVLAIVANAVVTRPGYELRTDHVSDAVRERIIDLRG
                     EVNAEDVRAAEMSGEPRIYFTDAVKMDISSSNIRRAVRAAGADEKLEGLIQVPQPVAE
                     YIKKYRLYREAHETERNDAGRERAHH"
     gene            complement(2742..3875)
                     /gene="obgE"
                     /locus_tag="VGB76_07280"
     CDS             complement(2742..3875)
                     /gene="obgE"
                     /locus_tag="VGB76_07280"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY0098742.1"
                     /translation="MFIDRVKIRVSGGHGGNGVTAFRREKFVPRGGPSGGEGGKGGDV
                     WLVADTGVNTLLHLRYNPEHHADRGRHGEGSNRSGREGEDIVVRVPIGTQIFDAETGD
                     LMCDLAEEGQRWLAAKGGRGGFGNSHFATSTNRAPRYHQEGSPGEERELQLELKLLAD
                     VGLVGFPNAGKSTLISIISAAKPKIADYPFTTLEPHLGVVDLGDYRTFVVADIPGLIE
                     GAHEGAGLGDRFLRHVERTKLLLHLVDVSSSSGREAVGDYEAINRELLAYDPQLAERP
                     QIVVATKIDALDEPERLENLRRRAREDGRDFYAISSVTNTGVRELVFAVSQALDAMRV
                     GDAATATRETSAAAAGAGSDLTAMSDSSNSSGNGSDGSTAVIE"
     gene            complement(4106..>4313)
                     /locus_tag="VGB76_07285"
     CDS             complement(4106..>4313)
                     /locus_tag="VGB76_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY0098743.1"
                     /translation="GNILARQRGTKWKPGKNVGRGKDDTLFALIDGFVKFEDKGRQGK
                     FISVYTEATQGNAPAPAPEATAAA"
BASE COUNT          798 a         1429 c         1218 g          868 t
ORIGIN      
        1 tacggcaacc cgtgcataat ggtgtacgca cgataaagct gttcgaccag caccactcgc
       61 gccatctcat gcgtcagagt cagacgcgac atagaccagc gcacttgcgc ttttgctttc
      121 aactccggcg cgactccgag atgcccgccg atgacaaacg cgatctcttt ccgcccgtcc
      181 aactgccaac gctcaacctg cgccgcgagt tccggcgaac tccactcgcg cgctccctcc
      241 acatccagca acaccgtcaa cgcaccaccc gcgccgagcg cgcccgcgat ccgcttgctc
      301 tcttcctcta tgacggctcg ctcatccccg ctcgcgctct cgcgcaactc cgtcacttca
      361 caacgaacga aacgtcccaa acgctgcaag taatcatcca cgagcgcacg cacatgctca
      421 ttcttcgtct tgcccaccca gatgagacga atacgcatcg ttcaagggat gaaggatgag
      481 gaatgaggga tgaacaagaa aactactcaa gctattcatc ctgtctgatt catccctcat
      541 ccttcatcgt tccccttctg ccgcgccagt ctctcgtaga gattcttgcg gctgatgccg
      601 agcaggcgcg cggcctcgga tttattgccg cgcgtcgaag cgagcgtctc cgcgatatag
      661 gcggcttcga gttcggcgag cgtcatccgt tcaacccgtc ttctttgcaa cgatgcggcg
      721 gcgcgcaccg cttcgggcag atgcgccgac gtgacgcgat ccccgtcggc cacgattacg
      781 gcacgctcta agacgtgcgc gagttcccgt acgttgccgg ggtagtcgta agtttctaac
      841 aacagcagcg cgtccggttc gatctgcgcg gcgcggcggc catgcttcga ggcataagtt
      901 ttcaaaaatc cccgcgccag ttttcgcacg tcctcgcggc gttcgcgcag cggcggcaca
      961 tggatgcgca cgacgttcag acgatagaac aagtccgggc gaaacgcgcg acgccggacg
     1021 gcctcatcca gatcgacatt cgtgagcgca atcaagcgcg catccacacg cacggcgcgc
     1081 ctgccgccca aacgctcaaa ctcgcgttct tcgatgacgc gcaaaagttt cgcctgtgcg
     1141 tcggtcgaaa gatgcgcgat ctcgtctaag acgagcgttc ccctgtgcgc cgcttcgagc
     1201 cgtccgggct tcgactctgt cgcgcccgtg aacgcgccgc gctcgtaacc gaacaactcg
     1261 gcctccaaca actccgcagg cagcgcggca cagtcaatct tcacaaacga cgcggcggcg
     1321 cgcttcgact gccggtgaat gtggagcgcg agcgcgtctt tgcctgcgcc ggactcaccc
     1381 gtgacgagaa cgttggcgtc cgtcgtcgcg acgcgactcg cgagacgcac ggcctcgcgc
     1441 atcgcctccg agttcgccac gagttcgatc aagattcgtt gctcaatttg tcactcgatg
     1501 cgccgctgct gctactgcct ctcgtctccg cctgcaagtc ggcgggcaac ggaacgcgca
     1561 cgccttcgcg ccagagtctt tcgagatcgt agaagcgtcg cgctttctct tcgaagatgt
     1621 ggacgatgaa gtcgccgtaa tcgacgagca cccactcagc cgtgcgatag ccttcgatgc
     1681 gttcggcgcg cgtgccctcc cgcttcaaac gctccgtgat ctcgtctgcg atggcctgca
     1741 cctgacgtgc gttcgtgccg ctcgcgatca caaaatagtc cgtgaaggtg gcgaccggac
     1801 gcagatcgag cactaccagg tcgagcgcct tcttgtcgct cgcggcgcgc aatgcgctct
     1861 caaggcgcgc gtccagacct tcctcttgtt gcggcgtcgc ggcagcgtcc gcgccgctct
     1921 cgtcgccgtg cgccgaagct gtagctgccg ccgttgctgc ggcgtcggcg gtgtccggtt
     1981 ctttcttcaa tgtcgcgcgc tttttagtgg tgagcgcgct ctcttccggc gtcgtttcgt
     2041 tctgtttcat gcgcctctct gtagagcctg tattttttga tgtactccgc tacaggctgc
     2101 ggaacttgaa tgagtccttc cagtttctcg tctgcgcccg ccgcgcgcac ggcgcgacga
     2161 atattgctcg aagagatgtc cattttcacc gcatcggtga aatagattct cggctccccc
     2221 gacatttccg ccgcgcgcac atcctccgca ttcacttcgc cgcgcaggtc tatgatccgc
     2281 tcgcgcaccg cgtccgagac atgatccgtg cgcagttcat aaccgggtcg ggtcacgacg
     2341 gcgttggcga ctatcgccag cacacgctcc cactcgcgcc acgtcgtgat ctcctcccac
     2401 gaatccgcgc ccatcacaaa caacaactgc ccgcccggag catcccgcag acgcaagatc
     2461 gtctcgatgg tgtaaggctt cctcggttca tcgagttcga ccgtggagat gcgaaaacgc
     2521 ggttcgtccc tggtcgccag cgcgagcatc gcgtaacgat gataggcgga agcgggcggg
     2581 acgtttcgct tgtgcggcgc gacatgcgcc gggatgaaaa gaacttcgtc gagcgagaac
     2641 aacttcaaca actcgcgcgc cacggcgaga tggccgttat gcacgggatc gaacgtgccg
     2701 ccgtagagcg cgatgcgaga atggcgcggc caattcataa ctcactcgat cactgctgtc
     2761 gaaccgtcgc tgccgttacc gctactgttg ctgctgtcgc tcatggcggt caggtcggaa
     2821 cccgcgcccg ccgccgcagc acttgtctct ctcgtcgccg tagcagcgtc gcccacgcgc
     2881 atcgcgtcca acgcttgaga gacggcgaag acaagttcgc gcacgcccgt attcgtgacg
     2941 gacgagatgg cataaaaatc ccgcccgtcc tcgcgcgcgc ggcggcgcaa attttccaga
     3001 cgctccggct cgtcgagcgc gtcaatcttc gtcgccacaa cgatctgcgg gcgttcggcg
     3061 agttgcggat cgtatgcgag caactcacga ttgatcgcct cgtaatcgcc gaccgcctca
     3121 cgccctgacg aagaagagac atccacgaga tgcagcagca acttcgttcg ctcgacgtgg
     3181 cgcaggaagc gatcaccgag ccccgcgcct tcatgcgcgc cttcgatgag gccggggatg
     3241 tctgcgacga cgaaggtgcg atagtcgccg aggtcaacga cgccgagatg cggttcgagc
     3301 gtggtgaaag gataatcggc gatcttcggc ttggcggcgg agatgatcga gatcaaagtt
     3361 gacttgcccg cgttagggaa accgacgagg ccgacatcgg ccaggagttt aagttcgagt
     3421 tgcaactcgc gctcttcgcc ggggctgcct tcctgatgat aacgcggcgc gcggttggtg
     3481 gaggttgcga agtgtgaatt gccgaaaccg ccgcgacccc ctttcgccgc cagccaacgc
     3541 tgcccctctt cggcgaggtc gcacatcaaa tcgcccgtct ccgcgtcgaa gatttgcgtg
     3601 ccgatgggca cgcgcaccac gatgtcttcg ccttcgcgcc ccgaacggtt ggagccttcg
     3661 ccgtgccgcc cgcgatccgc atggtgttcg gggttgtagc gcaggtgcag gagcgtgttg
     3721 actcccgtgt cggccacgag ccacacgtcg ccgcccttgc cgccctctcc ccccgaagga
     3781 ccaccgcgcg gcacaaactt ctcgcgccgg aaagcggtca cgccgttgcc gccgtgtccg
     3841 ccgctcacgc gaattttgac tcgatcaata aacatagcga taaacgatgc gcgcgctacg
     3901 atgaatgaaa gcaggagaca gaattcagaa cacagaagtc agaataaaac atgtcgggct
     3961 tccttctgac ttctgtgttt cggattctgt tttctgagtc atccttcatc attctgcgtt
     4021 catcattaaa aggaaaggac gccctacgct tgttcgttcg agcaggcgcg ggcgtcgcag
     4081 ctaactggtt cggataaccc cttggctaag cggcagcagt agcctccggc gcgggggctg
     4141 gagcgttgcc ttgagtcgcc tccgtgtaga cgctgatgaa cttgccctgc cgtcccttgt
     4201 cctcaaactt gacgaagcca tcgatcaagg caaacaacgt gtcgtctttg ccgcgaccga
     4261 cgttcttgcc cggcttccat ttcgtgccgc gttgacgcgc taaaatgttg ccg
//
LOCUS       DASWNS010000214         6760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_137961, whole genome shotgun sequence.
ACCESSION   DASWNS010000214 DASWNS010000000
VERSION     DASWNS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137961"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VGB76_07290"
     CDS             complement(<1..916)
                     /locus_tag="VGB76_07290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0098744.1"
                     /translation="MMSRRPTVSICIPTFNGAKYLHQAIESALAQSLTDFELLIVDDA
                     STDDTLSIAQAFARRDARIRVHQNSRRLGLGGNWNRCLELAGGEWIKFLFQDDYLAPE
                     CVARMYELGSARGATLVACDRDFVFEEGVPASFRELFLRYVSEHNLSVRFPGNQGHIS
                     NIEFAALVAEYPTENCIGEPTSVMFLRSACGRFGSFNTDLGQLIDWEYWMRIGVNEGL
                     CFLDEPLAAFRLHRQAATLSNQSESGIRVVGVDALIIRHELVYNPFYEPVRVAARPGS
                     LKQKLFAQYHGTRALAREVASQRDPADSP"
     gene            complement(1097..1828)
                     /locus_tag="VGB76_07295"
     CDS             complement(1097..1828)
                     /locus_tag="VGB76_07295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0098745.1"
                     /translation="MIVFAERVIKPYIEASGYRRVCEIGASRGQTTDKILEIDSVSID
                     IIDPCLDADLRQKYRERERVKVHQGISLDVLKRISGTFDCILIDGDHNWYTVYHELRT
                     IEERSLIRNGGTIFLHDVGWPYGRRDLYYQPELIPEEAIHPYRRAGLVYGESELSDDS
                     DINAELCNAVYEGGPRNGVLTAIEDFFYEHKGKYKFFLFEEEYGLGVLLRTDGTLGSI
                     SFQRYFLEAKLRRLLRRLKNLIRQY"
     gene            2241..5021
                     /locus_tag="VGB76_07300"
     CDS             2241..5021
                     /locus_tag="VGB76_07300"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0098746.1"
                     /translation="MKRQTVLTLVLLFANIFQVAPPQALAQQTNKPAATGQFDRTRYA
                     IPHKKFVLDNGLTLLVHEDHSVPIVGVNLWYHVGSRNEKRGKTGFAHLFEHFFFNGSE
                     NYPHGFREAMDDLGANNRNGTTNSDRTNFFEDVPVSALERTLYLEADRMGFLANYISK
                     EMLERERGVVQNEKRQGENQPYGRVFDEIGQKIYPYSHPYSWSTIGSMEDLNAATLDD
                     VKEWYKTYYGPNNAVISLAGDITPERALELVNKYFGGIPPGPALQRTEKWIPTLDRNI
                     RDEMQDRVPQARVYRVYHAPAWKETELQHLSLFADVLANSKDSRLARRLVYDKKSATD
                     VRASVFDSELGSIFLVTASVKPEANPAEVERDMDAVINEMLEQGASEEELRRMKNVNL
                     AGFLRGAERLGGFGGRSDILAESMTYGGSPDYYLQRLEAMANATPADVKTAANRWLRA
                     NHYTMTVKPFPRLAAGKSDVDRKILPPLGAAPDVKFPALQRAKLKNGLNVLLLERHAT
                     PIVNVALALDAGSSSDTPDKAGVATLALDLLDEGTKTRDAFQIEKELDLLGANLFTGS
                     SLDMSFVRLQATAENLRPSLGIMADVVLNPSFPADKFLIQKQRRLAQIGQEKANPNAL
                     AQRILPGIIYGANHAYGKPASGFENTVATISREDLMKWHADWFKPGSATVVVTGDTTL
                     DKVLPALEEALGGWQQGTAPRKNLGTVARTTGKKIYLIDKPNAPQSTIVAAHVSETSG
                     QAEDLALEPVMQNFGGMATSRLNRNLRLDKHWSYGTSGQVTSVRGQRTFLVLAPVQTD
                     KTKESIVEVTKEINNIAGARPVSGEEFTSIMRNMTSRLAGRFETISALESAAIAQLNL
                     NLPADYWANYSKNLRSLSEAQLDAAARKFVRPGELVWIVIGDLSKIEPGIRELNLGEV
                     VRLDADGKPFTR"
     gene            5253..6254
                     /locus_tag="VGB76_07305"
     CDS             5253..6254
                     /locus_tag="VGB76_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098747.1"
                     /translation="MNLFERTHLKATNAAVVVAAFLVLTCSTHVPAQDKSEKKRALAG
                     TPILWQEPGDIASRNLLHGAGGARMRPNLSRLTFIKEEKGGWSKKYRVRDGAGRVWVA
                     KIGKEAQSETASTRLLWAAGYMTEITYLVPRVRIPGKGVFQNVRFEARPENIEREGIW
                     TWENNPFVGTRELQGLKVMMVLLNNWDTKDENNVVLAVPGRDGTELRYAISDLGATLG
                     DTGKWPLLWRFTRSRNDPKGFRGDKLIDEIKEDGRVDFEFSGKKRGIYNDITVEQAGW
                     LGRLLARLSDAQLRDAFRAANYSPAEIRTLSRAVRARINELANLPRRAAARSKAAGR"
     gene            complement(6395..>6760)
                     /locus_tag="VGB76_07310"
     CDS             complement(6395..>6760)
                     /locus_tag="VGB76_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098748.1"
                     /translation="HPPYSSGGKHGSSSGVREVVEPIFLKYGVNAVFNGHEHFYERIK
                     PQKGIYYFISGAGGKLRPGDVKDRSPLTEKAFDRDMSFMLLEISGDEMHFQVITRTGE
                     TVDSGVLSRQPQKTSSAAN"
BASE COUNT         1518 a         2048 c         1870 g         1324 t
ORIGIN      
        1 ccgggctgtc tgccgggtcg cgttgactgg caacctcgcg ggctaaggct cttgtgccgt
       61 gatactgggc aaaaagtttt tgcttgagcg aaccggggcg cgccgccaca cgcaccggct
      121 cgtaaaaggg gttatagact aattcgtgcc ggatgatcag cgcatccacg ccgacgactc
      181 taatccccga ttcggattga ttcgagagcg tcgcggcctg cctgtgaagt ctaaaggcag
      241 ccaacggctc gtcgagaaaa cagagtccct cgttgacgcc tatcctcatc caatattccc
      301 aatctatgag ctgcccgaga tcggtattga agctaccgaa gcgtccgcac gccgaacgca
      361 ggaacatcac cgacgtgggt tcgccgatac aattctccgt aggatattcc gcgacgagcg
      421 cagcgaattc tatattactt atatgtccct gattaccggg gaaccgcact gacaggttgt
      481 gctcggagac gtagcgcagg aagagttctc tgaaagaagc ggggacaccc tcttcaaaaa
      541 cgaagtccct gtcgcacgcc acgagcgtag ccccgcgtgc ggagccaagc tcatacatcc
      601 gcgccacaca ctcaggggct aagtaatcgt cctgaaagag gaacttgatc cactcaccgc
      661 ctgccaactc aaggcaacgg ttccagttgc ccccgagtcc gagccgacgc gaattttgat
      721 gaacccgtat tctcgcgtct cgccgggcga aggcttgcgc gatggagaga gtatcgtcgg
      781 ttgaggcatc gtcaactatg agcagttcga agtcggtcag actttgcgcg agtgcgctct
      841 cgatagcctg atgcaaatat ttcgccccgt tgaaagtcgg aatgcagatg ctaaccgtgg
      901 gtcttctact catcatcgtc tttcgtggca cacgctctca tcttttcgtg accgggcgaa
      961 atcttcagaa cgattttcca gacggcttct gacaggcggg gcgaatgcac atacttcacc
     1021 cctgctgttg aatacctctc tgccgatctc ttcaaccatc tatttaatag ctttttgggg
     1081 gaaggtagag aatagactaa tactgccgga taaggttctt caacctgcgc aggagtctgc
     1141 ggagttttgc ttcgaggaag tacctttgga aactgatgct gccgagtgtg ccgtcggttc
     1201 tcaggaggac gcccaacccg tactcttctt cgaaaaggaa gaacttgtat ttccctttgt
     1261 gctcataaaa aaaatcttca atcgccgtta agaccccatt tcgagggccg ccttcataga
     1321 ccgcgttaca aagttctgcg ttgatgtcgg aatcatcgga caactccgat tccccataga
     1381 ccagccctgc ccgcctgtac gggtgaatcg cttcttcggg tatcaactcc ggctggtagt
     1441 acagatctct tctgccgtaa ggccagccga catcatgcag aaagatggtg cccccatttc
     1501 tgattagact tctctcttca atcgtccgca gttcgtggta gaccgtatac caattatggt
     1561 cgccgtcaat aagtatgcaa tcaaacgtcc ctgatattct tttaagaaca tcaaggctga
     1621 tgccctgatg aaccttaacc ctttcgcgct cccggtattt ttggcggagg tctgcgtcaa
     1681 ggcagggatc aatgatatca atcgacaccg aatcaatttc cagaatctta tcggtagtct
     1741 ggcctcggct cgcgcctatc tcgcacacgc gccggtatcc gctcgcttcg atgtagggtt
     1801 ttataactct ctccgcaaaa acaatcatcg agactccccg gcgtcattaa cagatgacta
     1861 caaatacagt ccactattcc gaccgaatgg cgcggcctga ctccctcaca tgatacatcc
     1921 gcgcgctttc gttcgacttc cgacacgcga gcgaaagcct gacggggcag cctgccgctt
     1981 ctaattctcg gaacacattt tcattgtcaa caagcgttcc gccgcgcgct tctctttgct
     2041 tctcttacat cgcaaatatc tactatctac cgattcgcag gcagcgcgca aagctgcgcc
     2101 gacgggacgc ctgccgctaa ataaggattg tgttttggtc gcggattaca gtaaaagtat
     2161 cgcagcacaa tttccggcag gaaaactaac caactaaaaa ttgaatgcct tttcgggcag
     2221 ggtcagcgca ggagaatcgc atgaaacgtc aaactgtctt aactctcgtc cttttatttg
     2281 caaacatctt tcaggttgcg ccgccgcagg cactggcgca gcaaacaaac aaacccgccg
     2341 ccaccggcca gttcgaccgc acccgttatg ccatcccgca taaaaaattt gtgctcgaca
     2401 acggtttgac gctgctcgtc cacgaagacc acagcgtgcc gattgtcggc gtgaacttgt
     2461 ggtatcacgt cgggtcgcgc aacgagaagc gcggcaagac cggattcgcg cacctgttcg
     2521 agcacttctt tttcaacggc tcggaaaatt acccgcatgg cttccgcgaa gcgatggacg
     2581 acctcggcgc aaacaatcgc aacggcacga ccaactccga ccgcaccaac ttctttgaag
     2641 acgttcccgt ctccgccctc gaacgcacgc tctacctcga agccgaccgc atgggcttct
     2701 tagccaacta catctcgaaa gagatgctcg aacgcgagcg cggcgtggtg cagaacgaaa
     2761 aacgacaggg cgaaaatcag ccctacggtc gcgtcttcga tgagatcggc cagaagatct
     2821 atccctactc gcatccctat tcatggtcaa ccatcggctc gatggaagac ttgaacgcgg
     2881 ccacgctcga cgacgtgaaa gaatggtaca aaacttatta cggacctaat aacgcagtga
     2941 tctcgctcgc gggcgacatc acgcccgaac gcgcgctcga actcgtcaat aaatatttcg
     3001 gcggcatccc gccgggaccg gccttgcagc gaaccgaaaa atggattccc acgctcgacc
     3061 gcaacatccg cgacgagatg caagaccgcg tgccgcaagc gcgcgtttac cgcgtctatc
     3121 acgcgcccgc ctggaaagag acggaattgc agcacctgag cctgttcgca gacgtgctgg
     3181 caaactccaa agattcacgt ctcgcgcgcc gtctggttta cgacaaaaag agtgcgaccg
     3241 atgttagagc gagtgtcttc gatagcgaac tcggcagcat tttcctcgtc acggcatcgg
     3301 tcaaacccga agccaatccg gcggaggtcg agcgcgacat ggacgccgtg atcaacgaga
     3361 tgctcgaaca gggcgcgagc gaagaagagt tgcggcgcat gaagaacgtg aacctcgcgg
     3421 gttttctgcg cggcgcagag cggctcggcg gcttcggcgg gcgttcggac attctcgccg
     3481 agagcatgac ttacggcggc tcacccgact attacttgca gcgtctggaa gcgatggcga
     3541 acgccacgcc tgccgacgtg aagacggcgg cgaaccgctg gctgcgcgcg aaccattaca
     3601 cgatgacggt caaaccgttt ccgcgtctgg cggcgggcaa atccgacgta gaccggaaaa
     3661 ttttgccccc gctcggggct gcgcccgacg ttaagttccc cgccctgcaa cgcgccaaac
     3721 tcaagaacgg cctgaacgtc ctcctgctgg aacgtcatgc cacgcctatc gtcaacgtcg
     3781 cgctcgcgct cgatgccggg tcatcttcgg acacgcctga caaagcgggc gtcgccacgc
     3841 tcgcgctcga tctgttggac gaaggcacga agacgcgcga cgctttccag atcgaaaagg
     3901 agttggacct gctcggcgca aatctcttca ccgggagttc gctcgacatg tcattcgtgc
     3961 gcttgcaggc gacggctgag aacctgcgcc cgtcgctcgg aatcatggct gatgtcgtgt
     4021 tgaacccgtc gttccccgcc gacaaattcc tgatccagaa acaacgccgc ctcgcacaga
     4081 tcgggcagga gaaagcgaat ccgaacgcgc tcgcgcagcg cattctgccc ggcatcatct
     4141 acggcgcaaa tcacgcttac ggcaagcccg cgtcgggttt tgaaaacacg gtcgccacca
     4201 tctcgcgcga agacctgatg aagtggcacg ccgactggtt caaaccgggc agcgcaaccg
     4261 tcgtcgtgac gggcgacacg acgctcgata aagtcttgcc cgcgctcgaa gaggctctgg
     4321 gcggctggca acagggcacc gcgccgcgca aaaatctcgg cacggtcgcc cgcactacgg
     4381 gcaaaaagat ctacctgatc gacaaaccga acgccccgca atcaaccatc gtcgccgcgc
     4441 acgtctccga aacgagcggg caggcggaag acctcgcgct cgaacccgtg atgcagaact
     4501 tcggcggcat ggcaacctcg cgcctcaacc gcaacctgcg cctcgacaaa cattggagct
     4561 atggcacgag cgggcaggtg acttccgttc gcgggcagcg cacgttcctc gtcctcgcgc
     4621 cggtgcaaac cgacaagacg aaagagtcaa tcgtcgaagt gacgaaagag atcaacaaca
     4681 tcgcgggcgc gcgccccgtc agcggcgaag agttcacgag catcatgcgc aacatgacct
     4741 cgcgcctcgc cggaagattt gaaaccatct ccgcgctcga atcggcggcc attgctcagc
     4801 tcaatctcaa cctgcccgcc gattactggg cgaactactc gaaaaacctg cgctccctga
     4861 gcgaagcgca actcgacgcg gcggccagaa aattcgtccg cccgggcgaa ctcgtctgga
     4921 tcgtcatcgg cgatctgagc aagatcgaac cgggcatccg cgaactcaat ctcggcgagg
     4981 tcgtccggct cgacgccgac ggcaaaccct ttacacgtta actcgaagac cttcttaaat
     5041 tttcgtcgaa ggacagccgc cctcgcgcgg ctgtcctttt tttgccgacg cctgcgcgcg
     5101 aagagtccga cttgccgcgt tgaataacgc tctgcgcggg ggagatagac cgatggcctg
     5161 ccgattgtat aaaatccagc gcatccctcg tcagtttaga gatcgaagcg acgcccggct
     5221 ggtgtcgtat caaacggaga caaaaactca gtatgaattt atttgaacgg acgcacttaa
     5281 aggcaactaa cgctgccgtc gtcgtcgcag catttctcgt cttgacgtgt tcgacgcacg
     5341 ttccggctca ggataaatcc gagaagaaaa gagcgctggc gggcacgccc atcctctggc
     5401 aagagccggg cgacatcgct tcgcgcaatc tgctgcacgg ggcgggcggg gcgcggatga
     5461 ggccaaatct cagccgtctc actttcatca aagaggagaa gggcggttgg tcaaagaagt
     5521 atcgcgtgcg cgacggggcg gggcgcgtgt gggtggcgaa gatcggcaag gaggcgcaat
     5581 cggagacggc ttccacgcgc ctgctctggg ctgccgggta catgacggag atcacctatc
     5641 tggtcccgcg cgtgcgcatc cccggcaagg gcgtttttca gaacgtgcgt ttcgaggcgc
     5701 gccccgaaaa tatcgagcgt gagggcatct ggacgtggga gaacaacccg tttgtcggca
     5761 cgcgcgaact gcaagggctg aaagtgatga tggtgctctt gaacaactgg gacacgaagg
     5821 acgagaacaa cgtcgtgctg gccgtgccgg ggcgcgacgg cacggagttg cgctacgcca
     5881 tcagcgacct cggcgcgacg ctcggcgaca ccggcaagtg gcctctgctc tggcgtttca
     5941 cgcgcagccg caacgacccg aaaggctttc gcggagacaa actcattgac gagatcaaag
     6001 aggacgggcg cgtcgatttc gaattctccg gcaagaagcg cggcatctat aacgacatca
     6061 ccgtcgagca ggcgggctgg ctcggcaggc tgctcgcgcg cctgagcgac gcgcaactcc
     6121 gcgatgcttt ccgcgcagcc aactacagcc cggcagagat acgcaccctg agccgcgccg
     6181 tgcgcgcacg catcaacgaa ctcgccaacc tcccgagacg cgccgccgcg cgttcgaagg
     6241 ccgccggacg atagaccgga aaagagcatc gcgtgtggga caaataggat cgcccggcaa
     6301 catgtcgccg ggcgatccta tttgtccctc gcgggactcg cttatttgtc cctcgcgtga
     6361 cgcgtccaaa agagtctctt agcaaaagag tctcttagtt agccgcgctc gaagttttct
     6421 gcggttgcct cgacaggacg ccggagtcca cggtttcgcc tgtgcgcgtg atgacctgaa
     6481 agtgcatctc gtcgccggag atttcgagga gcatgaaaga catgtcgcgg tcgaaggctt
     6541 tctcggtcag cggcgagcgg tctttcacgt cgcccgggcg gagtttgccg cccgcgccgg
     6601 agatgaaata gtagatgccc ttttgcggct tgatgcgttc gtagaaatgc tcgtggccgt
     6661 tgaagaccgc gttcacgccg tacttcaaaa agatcggctc gacaacttcg cgcacgcccg
     6721 aactcgaacc gtgctttccg cccgaagagt agggcgggtg
//
LOCUS       DASWNS010000215         5090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_319474, whole genome shotgun sequence.
ACCESSION   DASWNS010000215 DASWNS010000000
VERSION     DASWNS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319474"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VGB76_07315"
     CDS             <1..398
                     /locus_tag="VGB76_07315"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY0098749.1"
                     /translation="NSMLGATHACANPLTQHYGTTHGEAIALMLPHVVRWNSSVVASR
                     YAELLRAAGRRDVDERSAGETLAARLDELKAAGALEPGAASVPEDALELLAAEAARQW
                     TGTFNPRPFDACGALSLYRRAFQIQPQNA"
     gene            412..1572
                     /locus_tag="VGB76_07320"
     CDS             412..1572
                     /locus_tag="VGB76_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0098750.1"
                     /translation="MKTESLSWSNRRTRFFVSLLLTFAFLLAPAASVSAQWADPNNWP
                     QFRGNNQLTGVSKADVPKNLRPLWSYEAGAGIESSAAIAYGTVFVGAQNGILAALDLS
                     NGAVRWKYNTNSKEGIGESSPAVYGSHVYVGDLGGTVHAVNAANGRGVWTYKTGGEIK
                     SSPVVVDDKVLIGSYDGHLYCLDARSGALRWKVKTNGPVHATAGVAGGTAYIAGCDEV
                     FRAIRISDGRELFNVVSGAYTGASPALMNNSAFYGTFDNDVLGVNLQTRRISWRYRNA
                     QRQFPFYSSAGAVENRIVLGGRDKYVHSLDARTGRAVWTFQTRARVESSPALASGRVF
                     VGSSDGRFYVLDFYSGAKLWEFDAGSALSASPAIASGRIVIGSQDGRLYCFG"
     gene            1784..3538
                     /locus_tag="VGB76_07325"
     CDS             1784..3538
                     /locus_tag="VGB76_07325"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEY0098751.1"
                     /translation="MKLHTISSGLLAVALAVLSNACVQTPTTNQAANTNAAAPTANAA
                     IPAELKPALDTINANDIMAHTKVLSSDEYEGRSPGTKGEELTVKYLTEQFQRLGLKPG
                     NPDGTYVQQVPLVGFTGAPRASFTAGGKTIDLKFPTDYVAVSRRFVPESKVENSDVVF
                     VGYGVVAPEYDWDDYKGVDVKGKTIVMLVSDPAVPDASDPAKLDEKMFKGKAMTYYGR
                     WTYKYEIARDKGAAAAIIIHETGPAGYGYEVVSGSWSRENFDVQTPNKNMDRAAIEGW
                     ITLDRAKELLSASGQDFDALKKAAVTKEFKPVTLNAKANFEIKNTIRNIESSNVVARL
                     EGSDAVLKNEYVVYSAHWDHLGRNPELAGDQIFNGALDNASGTAAMLEIAEAFTKMTP
                     APKRSIIFLAVTAEEKGLLGAKHYASNPLYPANKTLANINIDGVNQWGRTKDITMVGD
                     DNSTLIDILRETAGAQGRTVKPDPEPEKGYYYRSDHFEFAKVGVPALYTDAGTDYIGR
                     DEAYSKQKREEYDKNDYHKPSDQIKPDWELSGAVEDAQLLTLIGHRVAQGEKFPEWKT
                     GSEFKAKRDETMRGATTP"
     gene            complement(3881..4390)
                     /locus_tag="VGB76_07330"
     CDS             complement(3881..4390)
                     /locus_tag="VGB76_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HEY0098752.1"
                     /translation="MREINQAGLDLIKSFEGILDGDPSTANLDPYLDPVGIWTIGWGH
                     AIVVGKDFLRGKQNRAKAFSLYPGGLTIPEAETLLRADVMDKCRDVDSLVKVSLNDNQ
                     YAAVVSFTFNLGVGNLKQSTLLKKINAGDFAGAANEFAKWNKAGGKVLAGLTRRRAAE
                     AALFRRPVN"
     gene            complement(4440..>5090)
                     /locus_tag="VGB76_07335"
     CDS             complement(4440..>5090)
                     /locus_tag="VGB76_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098753.1"
                     /translation="RDTLREQSAGDDTLLLLLQRFAPLWLAGLLGAGIMAAVMASDSQ
                     ILALSTMFTEDIFAFYGGKRRFGESVQVQTGRIFIIIITVLAYAVALRAPETIFELAI
                     QYAFSGYAALSPLIIAALFWRGSTKWGALASTLWVALSVIAVAVFQSVVPAPAPGAAS
                     VYASIGNLEVLSRTPGGTAIFGFMPVVPMVLISALLMLVVSLVTRKPKAATIEKYF"
BASE COUNT         1068 a         1556 c         1581 g          885 t
ORIGIN      
        1 aaaattcgat gctcggcgca acccacgcct gcgccaaccc tctgacgcaa cactacggga
       61 cgacgcacgg cgaagccatc gcgctcatgc tgccgcacgt cgtccgctgg aacagttccg
      121 tcgtcgcctc gcgctacgcc gaactgttgc gcgccgcagg ccgccgcgat gtcgatgaaa
      181 gaagtgctgg cgagactttg gcggcgcggc tcgacgagtt gaaagcggcg ggcgcgctcg
      241 aaccgggcgc ggcgtccgtc ccggaagacg cgcttgagct gctcgccgcc gaagccgcgc
      301 ggcaatggac gggcacgttc aacccgcgcc cgttcgacgc gtgcggcgcg ctctcgctct
      361 accggcgcgc ttttcagatt caaccccaaa acgcttaagg acatggaaga agtgaagaca
      421 gaatctctct catggtcgaa ccggcggacg cgctttttcg tctccctcct tttaactttc
      481 gcctttttac tcgcgcccgc cgcgagcgtc tcggcgcagt gggctgaccc gaacaactgg
      541 ccgcagtttc gcggcaacaa ccagttgacg ggcgtgtcga aggccgacgt gccgaagaac
      601 ctgcgccctc tgtggagtta cgaagcgggc gcggggatcg aatcctcggc ggccatcgcc
      661 tacgggacgg tctttgtcgg cgcgcaaaac ggcatcctcg ccgcgctcga tctctcgaac
      721 ggcgcggtgc gctggaagta caacacgaac agcaaggagg gcatcggcga atcgtcgccc
      781 gccgtctacg gcagccacgt ctacgtgggc gacctcggcg gcacggttca cgccgtcaac
      841 gctgcgaacg ggcgcggcgt gtggacttac aagaccgggg gcgagatcaa atcttcgccc
      901 gtcgtcgtgg acgacaaagt tctcatcggc tcttacgacg ggcatctcta ttgcctcgac
      961 gcacggagcg gcgcgctgcg ttggaaagtg aaaacgaatg gtcccgtgca cgccaccgcc
     1021 ggagtcgcgg gcggcacggc ctacatcgcc ggatgcgacg aagtcttccg cgccatacgc
     1081 atctcggacg ggcgcgaact cttcaacgtc gtctccggcg catacacggg cgcgtcgccc
     1141 gccctgatga acaactctgc cttctacggc acgttcgaca acgacgtgct cggcgtcaat
     1201 ctacagacgc gccgcatctc ctggcgttac cgcaacgcgc agcgccagtt ccctttctac
     1261 tcctcggcgg gcgcggtgga gaaccgcatc gtgctcggcg ggcgagacaa gtacgtacac
     1321 agtctcgacg cgcgcaccgg acgcgccgtg tggacgttcc agacgcgcgc ccgcgtcgaa
     1381 tcgtcgcccg cgcttgcgag cgggcgcgtc ttcgtcggct caagcgacgg caggttctac
     1441 gtcctcgatt tctacagcgg cgcgaagctc tgggagtttg acgcaggctc ggctctttcc
     1501 gcctcgcccg ccatcgcttc cgggcgcatc gtcatcggct cgcaggacgg aaggctctac
     1561 tgcttcggat gaagccggga cacaggcaaa gagtaaagag taaagaggag aggtaaagat
     1621 aaaggtaaag ataaagaggc acgtccatca ggtgtcgctt tcttctacct ttagcccttc
     1681 gcctttccct tttcactccg gtcgcgtgtt tgacttgccg cacgtttcgg catagaaact
     1741 atttcgttca actttcaatt caattccagc gaggcacatt aaaatgaagc tgcacactat
     1801 ctcttccggc ctgctcgccg tcgcgctcgc cgtgctgtca aacgcgtgcg tgcagacgcc
     1861 gacgacgaat caggcggcga acacgaacgc cgccgcgcca accgccaacg ccgccattcc
     1921 cgccgaactc aaacccgcgc tcgacacgat caacgccaac gacatcatgg cgcacacgaa
     1981 agtgctctcg tccgacgagt atgaaggtcg cagccccggc acaaagggcg aagagttgac
     2041 cgtgaagtat ctgaccgagc agtttcagcg cctgggactc aagcccggca acccggacgg
     2101 aacctacgtg cagcaagttc cgctcgtcgg cttcacgggc gcaccgcgcg cttccttcac
     2161 ggcggggggc aagacgattg atctgaagtt cccgaccgat tacgtcgccg tctcgcgccg
     2221 cttcgtgccg gagtcgaagg tcgagaactc ggacgtagtc ttcgtcggct atggcgtggt
     2281 cgcgcccgaa tacgattggg acgattacaa aggcgtggac gtaaaaggca aaaccatcgt
     2341 gatgctcgtc agtgatccgg ctgtgcccga cgcgagcgac ccggcgaagc tcgacgagaa
     2401 aatgttcaag ggcaaggcca tgacctatta cgggcgttgg acctacaagt atgagatcgc
     2461 gcgcgacaag ggcgcggcgg cggccatcat catccatgag acgggacctg ccggttatgg
     2521 ttacgaagtc gtctcgggca gttggtcgcg cgagaacttc gacgtgcaga cgccgaacaa
     2581 gaacatggat cgcgcggcca tcgaaggctg gatcacgctc gaccgcgcga aggaactgct
     2641 gtcggcttcc ggtcaggact tcgacgcgtt gaagaaggcg gcggtgacga aagagttcaa
     2701 gcccgtgacg ctcaacgcca aggccaactt cgaaatcaag aacacgatcc gcaacattga
     2761 gtcgagcaac gtcgtcgcca gactcgaagg ttcggacgcc gtgctcaaaa acgagtatgt
     2821 cgtctactcg gcgcactggg atcatctggg gcgcaatccc gagttggcgg gcgatcagat
     2881 tttcaacggc gcgctcgaca atgcctccgg cacggcggcg atgcttgaga tcgccgaagc
     2941 cttcaccaag atgacgcccg cgccgaagcg ttcgatcatc ttcctcgccg tcactgccga
     3001 agagaagggc ttgctcggcg cgaaacacta cgcctccaac ccgctctacc cggcgaacaa
     3061 gacgctcgcc aacatcaaca tcgacggcgt caatcaatgg ggacgcacga aagacatcac
     3121 gatggtcggc gacgacaact cgacgctgat agacatcctg cgcgagacgg cgggcgcgca
     3181 aggccgcacc gtcaaacccg accccgaacc ggagaagggt tactattatc gttcggatca
     3241 tttcgagttt gcgaaggtgg gcgtgcccgc gctctacacg gatgcgggga cggactacat
     3301 cgggcgcgac gaagcctaca gcaaacagaa gcgcgaggag tatgataaga acgattatca
     3361 caagccctcg gatcagataa aaccggactg ggaactgtcg ggcgcggtcg aggacgcgca
     3421 actgctcacc ctgatcggcc atcgcgtcgc acagggcgag aagtttcccg aatggaagac
     3481 cggctcggag ttcaaagcca aacgcgacga gacgatgcgc ggcgcgacga ctccgtaaga
     3541 tgcgcgccgg aaaaattgaa ggggcaggga ttcgcgtccc tgcccctttt ttatttgaga
     3601 atgtccaccg cctcaacaac tcttgccgag gaagtagtag acgagaatga agatgagaat
     3661 ggttgagatg cagccgccgc cgagtttcca tgcggcgatc ccggcgatga ttccgcgcaa
     3721 aggcccttgc atgcagtttc ctccgatcag tttgcctgtc aacaacaatg tgaaccgtta
     3781 gagctacggt gtacggcaaa aaggacgggc tgtcaatggc ctcagactcg acccgcgcgc
     3841 aaactcgcgg acgcgcgtgc gcgcttcctg agggacggac tcaattgacg gggcgacgga
     3901 acagtgcggc ctctgccgcg cgacggcgcg tcagcccggc gagaactttg ccgcccgcct
     3961 tgttccattt cgcaaactcg ttggctgcgc ccgcgaagtc gcccgcgttg atctttttga
     4021 gcagcgtcga ttgtttgaga ttgccgacgc cgaggttgaa ggtgaagctc acgacggcgg
     4081 catactggtt atcgttgagc gaaactttga cgagcgagtc cacgtcgcgg cacttgtcca
     4141 tcacgtcggc gcgcaagagg gtttcggcct ccgggatggt caggccgccg gggtagagcg
     4201 agaaggcttt ggcgcggttc tgtttgccgc gcaggaagtc tttgccgacc acgatggcgt
     4261 gcccccagcc gattgtccag atgcccacgg ggtcgagata aggatcgagg ttggcggtgc
     4321 tggggtcgcc gtcgaggatg ccttcaaagc ttttgatgag gtcaagtccg gcttgattga
     4381 tttctctcat gtaagaactc ctgatgaagt gaagttaata aatccgggtt gtggatgaat
     4441 cagaaatact tttcaatcgt cgccgctttc ggtttgcgcg tgacgaggga gacgacgagc
     4501 atcagcaagg ccgagatcaa gaccatcggc acgacgggca tgaacccgaa gatggccgtc
     4561 ccgcccggcg tgcgcgagag cacttcgaga ttgccgatgg aagcgtagac gctggctgcg
     4621 cccggcgcag gcgcgggcac gacggattga aagacggcga cggcgatgac agagagcgcg
     4681 acccagagcg tcgaagcgag cgcgccccac ttcgtcgaac cgcgccagaa gagcgcggcg
     4741 atgatgagcg gcgacagcgc ggcgtagccg gagaaggcgt actggatcgc cagttcgaag
     4801 atcgtctccg gcgcacgcag ggcgacggcg taggccaaga ccgtgatgat gataatgaag
     4861 atgcgtcccg tctgcacctg aaccgactcg ccgaacctgc gcttgcctcc atagaaggcg
     4921 aaaatgtcct ccgtgaacat cgtggagagc gcgaggattt gcgaatcgga cgccatcacc
     4981 gccgccatga tgcctgcgcc gagaagcccc gcgagccaga gcggcgcgaa gcgttgcagc
     5041 aggagcagga gggtgtcgtc gcccgccgat tgctcgcgca aggtgtcgcg
//
LOCUS       DASWNS010000216         9549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_346124, whole genome shotgun sequence.
ACCESSION   DASWNS010000216 DASWNS010000000
VERSION     DASWNS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9549
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346124"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="VGB76_07340"
     CDS             complement(<1..1565)
                     /locus_tag="VGB76_07340"
                     /inference="COORDINATES: protein motif:HMM:NF036398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098754.1"
                     /translation="MISVVNKSLHQLATTLALLSLCVLLASSSALAQDPKEDSTQIHT
                     GVFRASAVDSYLLMRQEWNGFLSQWQALEGNGYRMIDLETYTEGSKRLYAGIFRKGTD
                     AKGAIVGQEWAPFHAEWQNLEKKGLRMIDFETYTQGGKRLYAGIFRAGTYDAGAYIAK
                     PWDDFLAKWQEFEGKNFRLVDIEIYESGGKTLFSGIFHPGRDAKGALVGLDWGEFNKQ
                     AAGLEKLGLRLIDYETYTVGGKRLHAGVFRAGTDGRAIWGGVDEENFRGQIHNLSKGG
                     LGLSDVEIYSSPWEASCLNQVMPETGDAYTRNITSTPLHCEGQPGTCGTPGAKDTVRY
                     SAPWYPDGEQRYARISALYPADKFLTLPFDDPDVNYVGLWRYKGNGYHHAGDYSKGDK
                     TFQLRASAPGKVIHIGWDSWGGGTVIVSHDVGGKDTYRTIFRHVRNGADNDCDSAWKI
                     SVPTLSNDTLKDYKAYLEMSGCTEKKADRKLQEKFWGKNSEAIDMSLLGKDVTRGQAL
                     AWTGNTGPGGKKGT"
     gene            complement(2148..4868)
                     /locus_tag="VGB76_07345"
     CDS             complement(2148..4868)
                     /locus_tag="VGB76_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098755.1"
                     /translation="MTWWHRLVRRRKYEEELEKELRFHLDQHTDDLIAQGYSPAEARR
                     QARLALGGTEQVKEKCRDARGVRLLEDLLQDLRYAVRVLTKSPAFTLVSVLSLALGIG
                     VNATVFGFVNAVLLRPLSLPDHAELVRIQDDNLPAYSDYLAFNERAPVFRGLTAYDFD
                     SFQLTAGDSSARADVVLVSGNYFDVLGVRPALGRAFSADEGARPGAAAVAVISHGMWQ
                     GRFSADPAVIGKTFSLRRVPFTIIGVAPREFTGVSLGRRHDLWIPFAAEVLLRPEGNR
                     IVNPDSYQVRVIGRLRPEVSLAQAQAAVEIVAAQQDQVRKVRLFTDDPVEQPSTPRAV
                     SRATAVEMGPRDRQQSWLAVAAVLTVVGLVLLAACANVANLLLGRAAGRRREIAVRLA
                     LGASRQRIVRQLLTESIVLSLLGAAAGLLLSRWVGDLLLSLLHWNSPSDVSASTLDLS
                     LDWRMLLYGMLVAVSTGVAFGLVPALQASKSDVVSDLKAETTARSPGRRRLTFRNALV
                     VTQIAISTLLLIPAGLLLRSVRLAETSGYGFTVADRYVAKVDLGSLGKDGRRQRLIEE
                     QLLGKVREMPGVRSATMSQIVPLSGGTMVSTLEVEGDAPGQQAAVFAENGAPDQPGSL
                     YLNMLDTRYFETMGIPLLAGRDFTERDDTTAPDVIIVNETLARRLAPDGQALGRRLIE
                     RDPMTGKPKPVEVVGVAKDAKYIWPSERPRYFAYRPVRQIVQSELGAAHLIVHATGDP
                     ATISKEVRSTANAIDPDLSVESVTRLDELIERHVGETKLVIWASAAVGALALLLGAVG
                     LYGVMAYAVAGRTKEIGIRMALGAGRGRVRRMILTEGLALAAAGTLAGLLISAASMRL
                     MRSLLYGISPIDPLTYATVATFLTAVALLSSYVPARRATKVDPMVALRSE"
     gene            complement(4865..5209)
                     /locus_tag="VGB76_07350"
     CDS             complement(4865..5209)
                     /locus_tag="VGB76_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0098756.1"
                     /translation="MKTKSDLPQGTLDLLILKIVALGAVHGYAIAQRLEQVSRGVVQV
                     PQGSLYPALHRLENRGLLAADWKATETGREAKFYRLTRKGRVQLETEAASWQRLTEAV
                     SLILKLSEGGAQ"
     gene            5793..6965
                     /locus_tag="VGB76_07355"
     CDS             5793..6965
                     /locus_tag="VGB76_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0098757.1"
                     /translation="MIYRLFALLLLMLCCGTARAGDPVVIGQTARIRSAVLNEDRNYQ
                     VYLPDSYGWAKDRRYPVLYVLDGMTHFRHTTGSIGYLAAHGEIPEMIVVAIASTVRIR
                     DFTQTDWPEVWVGGGGANNFKRFLSTELIPNIERTYRTDGFRVLSGHSAGGQFALYCL
                     TSEPSLFKAYIALSPSLDWDNNLPQRSLEKSFASTRSLPTFLYVARSDDSGRALADYE
                     RLVETLKTKSPQGFRWHSQAFPEETHSSIPLLAQVDALRRLYAGYRFHNDKLEKGFTF
                     AQQHFQDVSKTVGWPLAIPEDIINSLGYAALSEGKTQDAIALFKRNVESNPNSANAYD
                     SLADGYAKAGMWKDAARASDRAVALATEFNHPNLPYFIEQAKKMNERLKEKSENPK"
     gene            7438..7884
                     /locus_tag="VGB76_07360"
     CDS             7438..7884
                     /locus_tag="VGB76_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098758.1"
                     /translation="MTKTLFTVGWVLLLLLATALTLLSINSTYLPLAARTDDVTPSVT
                     IDDIGAKTSPEVATALRGRRVTAATWALAYGFMLWGVLLVPYKRGERWAWWTILLSIV
                     VPQLLSLARIPLLDTQQGASASAILIGMALVGLLLGARHVFGKRDS"
     gene            8114..9472
                     /locus_tag="VGB76_07365"
     CDS             8114..9472
                     /locus_tag="VGB76_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY0098759.1"
                     /translation="MFRIVIRAALCVAIAMCPAPASAQKGDNQRPNVVLIITDDVGYG
                     DLGSYGAPDVKTPNIDRLARDGVRLTDFYANGATCTPTRVGLISGRYQQRLGLEQPLG
                     AGGAKDWERGLPPTGESLPQLLKNNGYATALVGKWHLGWKSEFSPTAHGFDYFFGFKS
                     GFIDYYQHTAGADAPVKADLFENDRPVETPGYMTDLITERSVRFIEQNAGRPFFIDVS
                     YSAAHWPYQPPSASSTARDNARHLGPFDDPTGTRADYVAMLERADEGVGRIIKALDKA
                     GLRGNTVVIFTNDNGGEWLSRNTPLFHHKGSVWEGGIRVPAIVRWPGRIPAGRVSHQV
                     GITMDLTASILAATGTSVPPEARLEGINLLPVLEGRAKEIERTLFWRVAGTRTQQAVR
                     LGEWKMLLDGGRVMLFNLRADVGERNNLIGHRPDIARRLRPLLAAWQTDVDAEAKRMA
                     TR"
BASE COUNT         1841 a         2935 c         2903 g         1870 t
ORIGIN      
        1 gtgcccttct tgccgcccgg cccggtgttg cccgtccagg cgagtgcctg cccgcgcgtc
       61 acgtccttgc cgaggaggct catgtcaatt gcctccgagt tcttccccca gaatttctcc
      121 tgcaacttcc ggtcggcctt cttctccgtg cagcccgaca tttcgaggta cgccttgtaa
      181 tccttgagcg tgtcgttgga gagcgtgggc acggatatct tccaggcaga gtcgcaatcg
      241 ttgtccgcgc cgttgcggac gtggcggaag atggtgcggt aggtgtcctt gccgccgaca
      301 tcgtggctga cgatcacagt cccgccgccc caactatccc agccgatgtg gatgactttg
      361 cccggggctg acgcgcggag ttggaacgtc ttatcgcctt tactgtagtc gcctgcgtgg
      421 tgatagccgt tgcccttgta gcgccacagc ccgacgtagt taacgtccgg gtcgtcgaag
      481 gggagcgtga ggaatttgtc cgccgggtag agcgccgaga tgcgcgcgta acgctgttcg
      541 ccgtcggggt accagggcgc gctgtagcgc acggtgtctt tggcgccggg cgtgccgcac
      601 gtgccgggct gcccctcgca gtggagcggg gtgctcgtga tgtttctcgt gtacgcgtcg
      661 cccgtctccg gcatgacctg gttgagacaa ctcgcctccc agggagagga gtaaatttcc
      721 acgtccgata gcccgaggcc gccctttgac agattgtgta tctggcctcg gaagttctcc
      781 tcgtccaccc cgccccagat ggcgcgccca tccgtgccag cgcggaagac gcccgcgtgc
      841 aggcgcttgc ctcccaccgt gtaagtctca tagtcgatca ggcgcagccc caatttctcc
      901 aaccccgcgg cctgcttgtt gaattcgccc cagtcgagtc cgacgagcgc gcccttcgcg
      961 tctctgccgg ggtggaagat gccggagaaa agggtcttgc cgcccgactc gtaaatctcg
     1021 atgtctacca ggcgaaagtt cttgccctcg aactcttgcc acttcgcaag aaagtcgtcc
     1081 cacggcttgg cgatgtacgc gcctgcgtcg tacgtgcccg cgcggaagat gcccgcgtag
     1141 aggcgtttcc cgccctgcgt gtaggtttcg aaatcaatca tgcggagtcc cttcttctca
     1201 aggttctgcc actccgcgtg aaagggtgcc cactcctgtc cgacgattgc gcccttcgcg
     1261 tcggtgccct tgcggaagat gcccgcgtag aggcgcttcg acccttcggt gtaagtctct
     1321 aagtcaatca tgcggtagcc gttgccctcc agcgcctgcc actgcgagag gaacccgttc
     1381 cactcctgcc gcatcagcag atacgagtca acggcgctcg cgcggaagac gcccgtgtga
     1441 atctgcgtcg agtcctcctt ggggtcttgc gccagcgcgg aagacgaagc gagcaaaacg
     1501 cacagagaca gcagcgcgag agtggtggcg agttgatgaa gcgatttatt gacaacactg
     1561 atcatttctt attctccgtt cgagcgacac gttaaaaaac ttgtccgggc ggcgcgactc
     1621 tcgccgataa ccacacacca gacgtttgac ctcatccatt aatgcgttcg cagcgggcga
     1681 aagttatcat cggagacgaa ataatttttg ctcagcggag gcgagggcgg cgggtggagt
     1741 cgagtgaggg cggaatacgg gttggccggt tgtgttgcat gaagggttac aggattggag
     1801 cggcttgttt atccaacagc gagagtccca taattgcaga ttgagagact gatgatgccc
     1861 ggcgttagcg gtggaattag aatgaaataa gtatttagag acgcgaaagc tcttcaagtg
     1921 ttttctgcta tgcggcgacc cgaaagagac tgtagacaaa ttccacctgc ctcatgagat
     1981 cgcttccgct taatctcttg acctgacctt tacgaagcat gttcattgct tcaatgcctt
     2041 cgagtgttcg ctccgcaatt gcgacactgc cgtccggtgt tcgccccctt accgaatccg
     2101 taaagcagcc tcatcgtccc cttaaagtat ttctcgcgac accaccttca ttcagacctc
     2161 agcgcgacca tcggatcaac cttcgtcgcc cgccgcgccg ggacgtagct ggacaggagc
     2221 gctacagccg tgaggaacgt cgcgaccgta gcgtatgtca gcggatcgat gggggagatg
     2281 ccgtagagca ggcttcgcat gagcctcatg ctggcagccg agatgagcag ccccgcgagc
     2341 gtccccgccg ccgcaagtgc cagcccttcg gtgagtatca tccgccgcac gcgcccgcgc
     2401 cccgcgccga gcgccatgcg gatgccgatc tccttcgtcc ggcctgcgac cgcgtaggcc
     2461 atcactccgt acaggccgac ggcgccaagc agcaacgcca gcgccccgac ggcggctgaa
     2521 gcccagatga cgagtttcgt ctcccccacg tgtcgctcga tcaactcgtc gagccgggtc
     2581 acgctctcga ccgagaggtc ggggtcgatt gcatttgcgg tagatcgcac ttccttcgag
     2641 atcgtcgccg gatcgcccgt ggcgtggacg atgaggtggg cggcgcctaa ttcgctctgc
     2701 acgatctgcc tgacaggccg gtatgcgaag tagcgtggcc gctccgaagg ccagatgtac
     2761 tttgcatcct tagccacgcc gacgacctcc accggcttcg gcttccccgt catcgggtcg
     2821 cgctcgatca accttctgcc gagcgcctgc ccgtccggcg ctagtcgccg ggctaacgtc
     2881 tcgttgacga tgatcacgtc cggggctgtg gtgtcgtccc gttcggtgaa gtcgcgcccg
     2941 gccaggagtg gaatacccat cgtctcgaag tagcgtgtgt ccaacatgtt caggtagagc
     3001 gagccgggct gatcgggcgc gccattctcc gcaaacactg cggcctgctg ccccggcgca
     3061 tccccttcga cctccagcgt gctgaccatt gtgcccccgg acagcggcac gatttgcgac
     3121 atggtagcgg agcggacgcc cggcatctcg cgcaccttgc ccagcaactg ttcctcgatg
     3181 agtctctgcc gccgcccgtc cttaccgaga gatcccagat cgactttcgc cacgtagcgg
     3241 tcagcgacgg tgaagccgta gccggatgtt tcggctaagc ggacgctcct gagcagaagc
     3301 cctgccggta tcaacaggag cgtcgagatt gcgatctgcg tcaccaccag cgcattgcgg
     3361 aaggtgagcc gtcggcgacc ggggctgcgc gccgtcgtct ccgctttgag atcggagacg
     3421 acatcggact tggaagcctg tagcgccggg acgaggccga aggccacacc cgtcgagact
     3481 gcgaccagca tcccgtagag aagcatccgc cagtcgaggc tgaggtcgag cgtactggcc
     3541 gacacatctg acggcgagtt ccaatgaagc agtgataaca aaaggtcgcc gacccagcgc
     3601 gacaggagca accccgccgc cgccccaagc agcgatagca cgatgctctc ggtcagcaac
     3661 tgccgcacga tccgttgccg gctcgctccc agcgcgagcc ggacggcgat ctcgcgccga
     3721 cgcccggccg cacgtccgag tagcaggttt gcgacgttgg cgcaggcggc cagaagcacc
     3781 agccccacga ctgtcaacac ggcggcgacg gccagccacg actgctgccg gtcgcgcggc
     3841 cccatctcta cagccgtcgc ccgcgaaacc gcgcgcggcg tggagggctg ctcgacgggg
     3901 tcgtcggtga agagtcgtac ctttcgaact tgatcctgct gcgcggcgac gatctcaacc
     3961 gcggcctgcg cctgcgccag actgacctcc ggcctgagcc tgccgatcac tctcacctga
     4021 tagctgtccg gattcacgat cctgttgcct tccgggcgaa gcagaacttc cgccgcgaag
     4081 gggatccaca ggtcatggcg acgaccgagc gagacgccgg tgaactccct cggcgccacg
     4141 ccgatgatgg tgaagggtac gcgacgcagg ctgaacgtct tgccgatgac ggcggggtca
     4201 gcgctgaacc tgccttgcca cataccgtgg ctgatgaccg ccacggcggc tgcgccgggt
     4261 cgtgcgcctt cgtcagccga gaaggcacgc ccgagcgcag ggcgcacgcc gagcacgtcg
     4321 aagtagttgc cgctgacgag cacgacatcg gcgcgcgccg acgagtcgcc cgcggtcaac
     4381 tggaagctgt caaagtcgta agcggtcagc ccccggaaga caggggcgcg ttcgttaaaa
     4441 gccaagtagt cggaataggc cgggaggttg tcgtcctgaa tgcggactaa ctcggcgtgg
     4501 tcgggaagcg agagtggccg cagcagcacg gcattgacga agccgaagac ggtggcgttg
     4561 acgccgatgc cgagggccag cgaaaggacg ctcacgaggg tgaaggccgg actcttcgtc
     4621 aggacacgca cggcatagcg caagtcttga agtaggtcct ctaacaagcg caccccgcgc
     4681 gcgtcgcgac acttctcctt cacctgctcc gtgccgccga gggcgaggcg ggcctgccgt
     4741 ctcgcttctg cgggactgta gccctgggcg atcaggtcat ccgtatgctg gtctaggtgg
     4801 aagcgcaact ccttttcgag ttcctcttca tacttcctgc ggcgaaccag gcgatgccac
     4861 cacgtcattg ggcgcctcct tcagacagtt tgaggatcag actgacggct tcggttagtc
     4921 gctgccagct tgcggcttca gtctcaagct gcacccgccc cttccgcgtc aggcggtaga
     4981 acttggcctc acgcccggtt tcggtcgcct tccagtcagc cgccagcagg ccgcggtttt
     5041 cgagccggtg cagcgccgga taaagcgagc cttgcgggac ctgaaccacg ccgcgcgaca
     5101 cctgttcgag gcgttgcgcg atcgcatacc cgtgaacagc gccgagcgcg acaattttca
     5161 ggatcaatag gtccaacgta ccttggggca gatctgattt agtcttcata gttgatgcct
     5221 ccgtcttcta catgttatag aaacacctgt agaagcgcaa ggtatacttt agtacggtca
     5281 aatccacaaa atggttccat tcggatccta tcgaaagtgt atacccgtcg aggccgggtt
     5341 caggggaact gccctgaccg cctccaggca ctcggagact tcaggtgcga gcgcatcctt
     5401 ttatggtgcc gtggtctaca gtcagccctc gctctctcaa catctctttg aacggggatg
     5461 aggtatgctt caggggcggt tccaatgtga tcagttagaa gaacgtgagg gatggaatta
     5521 tatttgagag gctgttttca taacactgac cacaggatat ggcaggtgaa acttttttag
     5581 cacacgcgca atctaacaac cgtatgtacc agaccgcgag tcggcgtgaa tgtctccgag
     5641 agcttgggtg attccggggt tgcatgcgcg gcgggtgatg ccggggcgtt agggttcatc
     5701 aagatcgtca atgaacccat caaagctgcg tagaggcgac ttgatactga catcgtcctg
     5761 gagctaacga ctatcagagg atggagatga aaatgatcta tcgtctattc gcgctattgc
     5821 tattgatgct gtgctgtgga actgcccgtg cgggcgaccc ggtcgtgatc ggtcaaacgg
     5881 cgcgcatccg ttctgccgta cttaacgaag accgcaacta tcaggtctat ctgcctgaca
     5941 gctacgggtg ggcgaaggat cgacgctacc cggtgctata cgtactcgat ggcatgacgc
     6001 atttccgcca cacgacgggt tccatcggct accttgccgc gcacggagag atcccggaaa
     6061 tgatcgtggt cgcgattgcg agcaccgtgc gtatcaggga tttcacgcaa acggactggc
     6121 ctgaagtatg ggtcggcgga ggcggtgcga acaacttcaa acgcttcctg tccaccgagc
     6181 tgataccgaa cattgaacgg acttatcgca cggacgggtt ccgagtgctc tccgggcatt
     6241 ctgccggtgg gcagtttgcc ttgtactgtc tgacctctga gccgtcgctt ttcaaagcgt
     6301 atattgcgtt gagtccgagc ctcgattggg acaacaacct gccgcaacgg tcgctggaga
     6361 agtccttcgc atcgacccgc agtttgccga ccttcctgta cgtcgcgcgc tcggatgatt
     6421 cggggcgcgc tttggccgac tacgagcgac tggtcgagac gctcaagacg aaatccccgc
     6481 agggattccg gtggcacagc caggcattcc cggaagagac gcacagcagc attcccttgc
     6541 tcgcgcaggt cgatgccttg cgccgcctct atgcgggata caggtttcat aatgacaagt
     6601 tggagaaggg cttcacgttc gcgcaacaac attttcagga cgtgtcgaag acggtgggct
     6661 ggccgctcgc catcccggag gacataatca acagtctcgg gtatgccgcg ctatccgaag
     6721 ggaagactca ggatgctatc gcattgttca aacgtaatgt ggagagcaac ccgaattccg
     6781 ccaatgccta tgacagcctg gcagacggtt atgcgaaagc cggaatgtgg aaggatgccg
     6841 cacgcgcctc agacagagct gtcgccttgg ctactgagtt caaccatcct aatctcccat
     6901 acttcatcga gcaggccaag aagatgaatg aacgactcaa agagaagtct gagaatccca
     6961 aatgatccct aacaagctat cgcacctgac gcttcgatag catggctttg aagtaacttc
     7021 cccgcgtcgg tgcctgaatg attctcgccc ggcgcaggtg aaccgcggcg ttaaatttct
     7081 tcctctgtgc cgcagttgag cattcacacc taaccgacct catggtactt tatgcgcggc
     7141 gaggctacgg tgggcgcgtt gaccttgcag ccgtagctat gcaaagaggg tggcaaggga
     7201 gcagcacgcc agccctacca aattcttcac tcagcagttc gcccggaaac cgcggcgaaa
     7261 tctaacaacc atacacccga cccgaatcag gccggatgtc atcgagaact cgtctgtagc
     7321 gcagttattt gcgggcgggt gataccgggc accagacggc gatgccgtcc agagtgccgt
     7381 tcggttacaa ggccggtgta ctatgctcgc cacacgcccc gacagaaagg aacgccgatg
     7441 acgaagacgc ttttcaccgt aggttgggta ctgctgctcc ttctggctac cgcgctcacg
     7501 ctcctctcaa tcaactctac ctacctccca ctcgcggcca gaactgacga cgtgacgccg
     7561 tcggtcacga tcgacgacat cggagcgaag acttcccccg aggtggcgac ggcgctccgc
     7621 gggcggcgcg tgacggcggc gacgtgggca ctcgcctacg gtttcatgct ctggggggtg
     7681 ctcctcgttc cctacaagcg cggcgagcgc tgggcgtggt ggaccatcct gctctcgatc
     7741 gtggtcccgc agctcctctc gctcgcccgg atcccgctgc tcgacacgca gcagggggcg
     7801 agcgcgtcgg cgatcctcat aggcatggcg ctcgtcggcc tccttctcgg cgcccggcac
     7861 gtgtttggga agcgcgactc ctgaagcctt acgacgaccg gctgaaagcg cacgactctg
     7921 gcaaagcgtc ggctttgtgc cgtaaaactg agggcattct gcaccacctt taccggcgcg
     7981 acgcggagcc atttaacctt gcgaggctct tgtctatcag ggcggcaggc ggcggcttag
     8041 ctccagcgtt cggcagttcg gatttgcatg gtccgcgatg gtgttcccgc ggtcacttat
     8101 cataggaacg atcatgtttc gaatcgtcat ccgggcggca ctctgcgtcg ccatcgccat
     8161 gtgccccgcg cccgcctcgg cgcaaaaagg cgacaaccag cgtccgaacg ttgtcctgat
     8221 tattaccgac gatgtgggat acggcgattt aggcagttat ggtgcacctg acgtgaagac
     8281 gccgaacatc gataggctcg cacgtgacgg cgtccgtctg accgacttct acgcgaacgg
     8341 agccacctgc acgccgacgc gggtggggct tatctcagga cggtatcagc agcgtctggg
     8401 tctggagcaa ccgctcggcg ccgggggcgc gaaggattgg gaacgagggc tgccgccgac
     8461 gggggaatcg ctgccacaac tcctgaagaa caacggctat gctaccgcgc tcgtcggcaa
     8521 gtggcacctc gggtggaaaa gcgagttcag cccgactgcc cacgggttcg actatttctt
     8581 cggcttcaag agcggattca tagactacta ccagcacacg gcgggcgccg atgcgcctgt
     8641 gaaagcagac ctcttcgaga acgaccgtcc ggtagagacg ccgggctaca tgacagacct
     8701 gatcaccgag cgttccgtcc gattcatcga acagaacgcc gggcgtccgt tcttcatcga
     8761 cgtttcatac agcgcagcgc actggccgta ccaaccaccc agcgcgtcgt caacggcacg
     8821 tgataacgcc agacacttag gaccgtttga cgatccgacg ggcacgcgcg cggactacgt
     8881 tgcgatgctt gagcgcgcgg acgaaggtgt cggacgaatc atcaaggcgc tcgataaggc
     8941 tggcttgcgg gggaacacgg tcgtgatctt cacgaacgat aacggcgggg aatggttatc
     9001 gcgcaacacg ccgctcttcc atcacaaagg cagcgtgtgg gaaggcggaa tacgggtgcc
     9061 ggcaatcgtc cgctggccgg ggcggattcc ggcgggtcgc gtgtcacacc aagtcggaat
     9121 caccatggac ctgacggcct cgattctcgc cgcgacggga acgtcagttc ctcccgaagc
     9181 acggctcgaa gggatcaacc tgttgcctgt gctcgaaggc cgcgccaaag agattgagcg
     9241 gacgctcttc tggcgcgtgg cgggaacgcg cactcaacag gccgtacgct tgggcgaatg
     9301 gaagatgcta ctggacggtg gcagggtgat gctgttcaac ctgcgggcag atgtcgggga
     9361 acgtaataac ttgatcgggc accggcctga catcgcacgc cggttgcgcc cgctgcttgc
     9421 ggcgtggcaa acagatgtcg atgccgaggc aaagcgcatg gcgacgcgtt aacgcctgcc
     9481 cgttagccca tgtcattagt cgcgctggtt ggcgtactga gcagtgtcag ttgacgcggc
     9541 gtccggcgc
//
LOCUS       DASWNS010000217         5642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_477685, whole genome shotgun sequence.
ACCESSION   DASWNS010000217 DASWNS010000000
VERSION     DASWNS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477685"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VGB76_07370"
     CDS             complement(<1..915)
                     /locus_tag="VGB76_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098760.1"
                     /translation="MSRKSETIFENFLQQQDETAWARALETLLPAIHEVDRNATSIWF
                     AFFPLALARAFERADDPEKLAQTLLMQGRYRLADQIDSSHAFFYGHRYWPEVKRAVAA
                     HAARGTAAQGTLAEQIGEVAREVAAQKKVDESLLKGITAVAFMTLQQVGLEAFAAAPG
                     EVQLKGRSARQSPEKILQERAKDDRQGLLGNLLRTTDKRWTVVWNEHDPTARFQMIHR
                     QEVASAAATDKRDWSQVDPRCTVDEGPIPVQCRSASCGTCWVGVLGGAEKLTEVSPRE
                     RKAMQLFGYIDTDAPRPLIRLSCQAQGEG"
     gene            complement(1107..3248)
                     /locus_tag="VGB76_07375"
     CDS             complement(1107..3248)
                     /locus_tag="VGB76_07375"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0098761.1"
                     /translation="MSGLERFIGEVLDGKYRLERLLGQGGMGAVYLATHLGTERPVAL
                     KLITPEFMRNDEFVERFKREARAAGRLRHPNVVDVTDFGFAQVGTQRVAYLVMEYLDG
                     CTLGDVLAEESRLPIDWVVDILEQTCSAVDEAHQQGVVHRDLKPDNIWLEPNRFGGYR
                     VKVLDFGIAKLADAAVEGTTGDLAVAAANEPTVTNETPGTSQSGSQSPQSQLDIVQAS
                     IARAASALARSNPGSNLSNHGSSQEGSSEAATQLQLSNPTDAEQQQTRILAPSSTAQQ
                     AHDERTGIEHQPRSTQAAHESDQTRMLDVTGSGRRQNELLTASATAAGVTRVGAILGT
                     PLYMSPEQCRGQRLDARSDIYSLGVIAYQMLTGETPFAGDMMSVMRQHMESEPPPLRD
                     KNRKVKKKVARQVMAALKKNPAARPTSAAGFASALRGSSEGIGSLLRRGFALYSEHFP
                     KFLRISLLAHVPVIVVTLLMVGFDLLKRADKLSPTLQVVFGVGLALLNIVVSFLSTSI
                     ITAMTALIVTQLHIAPMRPISLRDALAVLRRRWRPFLRTSLRVSLTMGVGILMFVIPG
                     LIVMVLYYLYAPVVLMENLEKRAALKRANELSRRSRRSVIALIAINFALPLMMGAIVG
                     FLSVKVQGGNLHINQNSQILGRLAPLFNIITVPLISITTALLYLKMRQLGGETFKETI
                     EQFDNVEVPRSRWQERMRRRLTVNTPVSRGG"
     gene            complement(3465..3541)
                     /locus_tag="VGB76_07380"
     tRNA            complement(3465..3541)
                     /locus_tag="VGB76_07380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3505..3507),aa:Arg,seq:acg)
     gene            complement(3681..4820)
                     /locus_tag="VGB76_07385"
     CDS             complement(3681..4820)
                     /locus_tag="VGB76_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0098762.1"
                     /translation="MTDQRFFLRPIFQNISCAFVALLLCALFASPAHAQDDDDKVTVE
                     ANLIRLNVGVADARGRAVTDLTRNDFAVFEDGVPQTILNFEATTQPFSLVLLLDMSGS
                     TLSFRTQLKQSAQRFIDALSPADRVAVVSFWTEMKEQKKSVKPVDRIETLTNFTNDRM
                     RIAFAIDRAHGGGNTNLYKALRYSLDALKQEGTRRKAIIVLTDGIDSDVRREDRAATI
                     QAKTAEEAVAAIKPDANPTLAAVLNSADAQGVTIYPLALPSGDLKRLADRATLSITPN
                     KGTPPEDIKHLTQPTPQQAAIYSAARARLDTLASRTGGRLHEINRLEDMARLYAEVAA
                     EMRTLYTIAYQSAGSRPRDGGWRAIQIQVARPELIARTRPGYYAR"
     gene            complement(5067..>5642)
                     /locus_tag="VGB76_07390"
     CDS             complement(5067..>5642)
                     /locus_tag="VGB76_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098763.1"
                     /translation="YDEHREAVDTLVGDYYAGRRARDKPAPPASGVAASRMLPVVQVS
                     KPIATAVDMSELIRLEVKREMAEATFIWIDKEGRAELLGHDEVLRRAARGTKPQTIYK
                     VAQEMELKFQLVPRKRIERARRLAEVEEERAVARRTRRTAATNARNASTPAFDELDEE
                     SPASSSSNIAAASAVAAPRRTRRPRTLKAVS"
BASE COUNT         1009 a         1702 c         1817 g         1114 t
ORIGIN      
        1 gccttcgccc tgcgcctggc aggagagacg tatgagcggg cgcggcgcgt ccgtgtcgat
       61 gtagccgaag agttgcatcg ccttgcgctc gcgcgggctg acctcggtta gcttctccgc
      121 gcctcccaac acgccgaccc agcacgtccc acacgaagcc gaacggcatt gcaccgggat
      181 gggtccttcg tccacggtgc agcgcggatc gacctgcgac caatcgcgct tgtccgtcgc
      241 cgctgcggaa gcgacttcct gccggtgaat catctggaag cgcgccgtcg ggtcgtgttc
      301 gttccacacc accgtccaac gcttgtcggt cgtgcgcagg aggttgccga gcaagccctg
      361 tctatcgtcc ttcgcgcgtt cctgcaaaat cttttcgggc gactgtcgcg cgctcctgcc
      421 cttgagttgc acttcgcccg gcgcggcggc aaacgcttcc agcccgacct gctgcaaggt
      481 cataaaagcg accgccgtga ttcccttcaa gagcgactcg tcaaccttct tttgtgccgc
      541 gacctcgcgc gccacttcac cgatctgttc cgcgagcgtg ccctgcgccg ccgtgccgcg
      601 tgccgcgtgc gcggcgacgg cgcgtttaac ttccggccag taacgatgcc cgtaaaaaaa
      661 ggcgtgcgac gaatcaattt gatccgccag acgatacctg ccctgcatca acaacgtctg
      721 cgccaacttc tccgggtcgt ccgcacgctc gaaggcgcgt gccagcgcga gagggaagaa
      781 cgcgaaccag atcgaggtcg cgttacgatc aacttcatga atggcgggca acagcgtctc
      841 taacgcccgc gcccaagccg tctcgtcctg ctgctgcaag aaattctcga aaatagtctc
      901 actctttcta ctcatagcta cctctggaca atatgcactt caggtcaaac cgcaaagtgt
      961 agcccctccg ccccaaagtc tcaaacgcag aagcaacgat gaataaggag acagaacaca
     1021 ggagtcagaa gaaagagccg cgcacccttt attctgactc ctttattctg actcctgact
     1081 cctgcttttc attcctcgtt gcttcttcac cccccgcgac tgaccggcgt gttgacggtg
     1141 agacggcggc gcatgcgttc ctgccagcgg ctacgcggga cttccacgtt gtcgaattgt
     1201 tcgattgtct ctttgaaagt ctcgccgccg agttgccgca tcttcagata gagcagcgcg
     1261 gtggtgatgg agatgagcgg cacggtgatg atgttgaaga ggggcgcgag ccgtccgagg
     1321 atttgcgagt tttgattgat gtgcaggttc ccgccctgca ccttcaccga gagaaagccg
     1381 acgattgcgc ccatcatcag cggcaacgcg aagttgatgg cgatgagcgc gatgacggag
     1441 cggcgcgaac ggcgcgaaag ctcgttggcg cgtttgagcg cagcccgctt ctcaaggttc
     1501 tccatcagga cgaccggcgc atagagataa tagagaacca tcacgatgag gccggggata
     1561 acgaacatca ggatgccgac ccccatcgtc aggctcacgc gcaggctcgt gcgcaaaaac
     1621 ggtcgccagc gtcgccgcaa aacggcaagc gcgtcgcgca gagagatggg gcgcatcggc
     1681 gcgatgtgca gttgcgtcac gatgagcgca gtcatcgccg tgataatgga ggtggagaga
     1741 aaactgacga cgatgttgag cagcgccagc ccgacgccga acacgacctg gagcgtcggc
     1801 gagagcttgt ccgcacgctt caacaggtcg aagccgacca tcaagagcgt gacgacaata
     1861 accggcacat gcgcgagcag agagatgcgc agaaacttcg ggaagtgctc gctgtagagg
     1921 gcaaagccgc gcctgagcag cgagccgatg ccttcggaac tgccgcgcag cgcagacgca
     1981 aaccccgccg cgctcgtcgg gcgcgccgcc ggattcttct tcaacgcggc catcacctgc
     2041 cgcgccacct tcttcttcac cttccggttc ttgtcgcgca gggggggcgg ttcgctttcc
     2101 atgtgctgcc gcatcacaga catcatgtcg cccgcaaacg gcgtctcgcc cgtcagcatt
     2161 tgataagcga tgacgccgag cgagtagatg tctgaacgcg cgtcgagccg ttggccgcga
     2221 cactgttcgg gcgacatgta gagcggcgtg ccgagtatcg cgccgacgcg cgtaacgccc
     2281 gctgccgtcg ccgaggcggt cagcaactcg ttctgcctgc gcccgctgcc cgtcacgtcg
     2341 agcatgcgcg tctggtcgct ctcgtgcgcg gcctgcgtgg agcgcggttg gtgctcgatg
     2401 cctgtgcgct cgtcgtgcgc ttgctgtgcg gtcgaagacg gcgcgaggat gcgcgtctgc
     2461 tgttgttcgg cgtccgtcgg gttcgacagt tgcaactgcg tcgcggcttc ggacgagcct
     2521 tcttgagacg aaccgtggtt ggataaattg ctgcccggat tcgaacgcgc gagcgcagag
     2581 gccgcgcgcg cgattgaagc ctgcacgatg tcgagttgcg actgcggcga ctgcgacccg
     2641 ctctgcgatg tgccgggagt ttcgttcgtc accgtgggtt cgttggcggc ggcgacggcc
     2701 aagtcgcccg tcgttccttc cacggcggcg tcggccagtt tggcgatgcc gaagtcgagc
     2761 accttcacgc ggtaaccgcc gaagcgattc ggctccagcc agatgttgtc cggcttgagg
     2821 tcgcgatgca cgacgccctg ctggtgcgcc tcgtcaaccg ccgaacaggt ctgctccaaa
     2881 atatctacga cccaatcaat cggcagccgc gactcttcgg ccagcacgtc gccgagcgtg
     2941 cagccgtcca gatattccat cacgagatag gccacgcgct gcgtgccgac ctgcgcgaag
     3001 ccgaagtcgg tcacgtctac cacgttcggg tggcgcaggc gtccggctgc acgcgcctcg
     3061 cgtttgaaac gctccacgaa ctcgtcgttg cgcataaact ccggcgtgat gagtttcaaa
     3121 gcgaccgggc gttccgtgcc gaggtgcgtc gccagataga ccgcgcccat cccgccctga
     3181 ccgagcagac gctccagacg atacttcccg tccagcacct ctccgatgaa acgctccaga
     3241 ccagacatat gtgccaaagc atccatgcgc ccgcgcgcaa agtcaagcga cgccccgagg
     3301 ccgaccgtct ccatgaaaga tttatacgcg cgaaggttat tcgtgaagct gaaccgaaaa
     3361 gttccatgcg ctgcgcgcta acacacttta agagttggca cgctcggcgc acacaacata
     3421 gaaaagggca gccgcgcggc tgtccttttc ctgttcgctt ttaatggtgc acccggcaag
     3481 attcgaactt gcgaccccat gattcgtagt catgtactct atccagctga gctacgggtg
     3541 cataaggcgt gaattgataa cgacgaatat acggaatggc tctcgcgggt gtcaagcgtg
     3601 cgtccgttga gcgtaaagcg gggcgcaagg cgcgccggaa cggatgcgcg gcgcgtgtga
     3661 tttcgcgttt cgtttgagcc ttagcgcgcg tagtagccgg gacgcgtgcg tgcgattagt
     3721 tcggggcgcg cgacttgaat ctggatggcg cgccagccgc cgtcgcgcgg gcgtgaaccg
     3781 gcggattgat aggcgatggt atagagcgtg cgcatctctg cggcgacttc ggcgtagaga
     3841 cgcgccatgt cttcgagacg attgatctcg tgcagacgcc cgcccgtgcg actggcgagc
     3901 gtgtcgagac gcgcccgcgc ggcggagtag atggcggcct gttgcggcgt cggttgcgtt
     3961 agatgtttga tatcttcggg gggcgtgccc ttgttcgggg tgatgctcag ggtggcgcgg
     4021 tcggcaagcc gtttgaggtc gccggagggg agggcgagcg ggtagatcgt cacgccttga
     4081 gcgtcggcgg agttgagcac ggcggcaagc gtggggttgg cgtcgggctt gatggcggcg
     4141 acggcttcct cggcggtctt ggcttgaatg gtggcggcgc ggtcttcccg ccgcacgtcg
     4201 ctatcgatcc cgtcggttaa gacgatgatg gctttgcgcc gtgtgccttc ctgtttgagc
     4261 gcgtcgagcg agtagcgcag ggctttatag agattggtgt tgccgccgcc gtgtgcgcgg
     4321 tcgatggcga aggcgatgcg catgcggtcg ttcgtgaagt tcgtgagcgt ctcgatgcgg
     4381 tctacgggtt tgacgctttt cttctgttcc ttcatctccg tccagaagga gacgacggcg
     4441 acgcggtcgg cgggcgagag cgcgtctatg aaacgttgcg cggactgttt gagttgcgtg
     4501 cgaaagctca gggtcgagcc ggacatgtcg agcaggagca cgagactgaa aggctgcgtg
     4561 gtcgcctcga agttgagaat cgtctgcggc acgccgtcct cgaagacggc gaagtcgttg
     4621 cgcgtaaggt ctgtcacggc gcggcctctg gcgtctgcga cgccgacgtt gaggcgtatg
     4681 agattggctt cgacggtgac tttatcgtcg tcgtcctgcg cgtgcgcggg gctggcgaag
     4741 agggcgcaca agagcagggc gacgaaggcg caggagatgt tttgaaaaat tggacgaagg
     4801 aaaaaacgtt gatcagtcat cggttctaag atcgcacgga gcataaaaat ttctgtcgcc
     4861 agtatgcaaa gggtcgcccg cgtttgcaag cagcgcgttc atacggcgta accctgaggc
     4921 agtggtcgtc acacgagcag gcaagattta gtctcgcacg cgaaacgtgg aaggtttcgc
     4981 gtgcgagact cttttggtgc tcggagcgtg gagcgcgttg cttgccgcag cagtaaggag
     5041 cgcgggcaag caggcttttg cgggtcttag ctgacggctt tgagcgtgcg ggggcggcgg
     5101 gtgcggcgcg gcgcggcgac ggcggaagcg gcggcgatgt tgctgctact gctggccgga
     5161 ctctcttcgt cgagttcgtc gaacgctggc gttgacgcgt ttctggcgtt cgtcgcggcg
     5221 gtgcggcgcg tgcggcgcgc gacggcgcgt tcctcttcga cttcggccag tcgtcgcgca
     5281 cgctcgatgc gcttgcgcgg cacgagttga aatttcagtt ccatctcctg cgcgactttg
     5341 taaatcgtct gcggcttcgt tccgcgcgcc gcgcggcgca acacttcgtc gtgtcccaac
     5401 aactccgcgc gaccttcctt gtcgatccaa ataaatgtag cttcggccat ctctctcttc
     5461 acctccagac gtatcaactc gctcatgtcc acggcggtcg cgattggctt cgaaacctgc
     5521 acgacgggca acatgcgcga ggcggcgacg ccggacgcgg gcggcgcagg cttatcgcgt
     5581 gcgcgccgtc cggcgtaata atccccgacg agcgtatcca ccgcctcccg gtgctcgtcg
     5641 ta
//
LOCUS       DASWNS010000218         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_192152, whole genome shotgun sequence.
ACCESSION   DASWNS010000218 DASWNS010000000
VERSION     DASWNS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192152"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1614
                     /locus_tag="VGB76_07395"
     CDS             55..1614
                     /locus_tag="VGB76_07395"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0098764.1"
                     /translation="MSHAFNFNPRRLLLRRLPTTRRAAIALTLAFVAVAACVLSARMR
                     VVYGSDQDLAKLNRFVQTGNTPAMKMFRAGRDLIENEEWAKAAETFRGFINTYPRDND
                     VDAALYWYAYALKRQGSTKEAAKALKRLIKDFQRSGWREEADAMLTELAPALGQTEII
                     DNALGKENEELKIIALQSLFESSPDRALAFVSEWLKPNSTASRRMKESAVSLLGAHGG
                     KQAIPLLLDIARHQPDAKLRQTAIHRLGDAGGEAVMDDLMRIYSSDPDPKIKQQVLHA
                     LAGMDGARASAKLLEIAQNNGEDLELRKTAIHRLGERDAAFDHLARLYDTDRSLEIRK
                     RLLNSFSESDDPRAAAKLLEVARTGDHVELRRSAIRRLGEKNSEAMLDELMRIYQGEQ
                     TLEVKRDILHAFSDMNNARAKSKLYEIARNPAENADLRRNVINRIAERNDPQAVELLI
                     SLYDAESNFDIKRTILNALAHSNQKAALRKLMQVARTDASLDLRRRAIQSIGESKDPE
                     ALRFLEDILKP"
     gene            1679..3403
                     /locus_tag="VGB76_07400"
     CDS             1679..3403
                     /locus_tag="VGB76_07400"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0098765.1"
                     /translation="MKDAQPATGTKQLATDNQLPTAPRKEFIMRRSLSFNSLFICLAL
                     LGGSACAHAGNAKPFEASASSVVETQASPSGAAQTSNFIQVEGADLRAKLDAAASRAR
                     QARTPYWSAYSFDVRPGIAIDPGMGEFHGSMNTSGGTTVFVGSSRGMTVETRNLAVFL
                     LRDAASGGAITRMEIYNLERKREFSGYPVYWLGRAGNEESLNYLRGIAEATPPPASTT
                     SAGLLNQHATLAIAIHDDARVSPLLKQFAGSSRHPKVRSTAVYWLGQIGGETDFLAGI
                     VRNNSETKDVRRQAAHAIGESRDRAALTTLQSLYEGMTERDVKRGIIHAVAGNENKEG
                     ALAFLLKVAKTDADKESRRTAVHAIGDEGRDSVIDELMSIMSSDQEQDVRRAVLHALA
                     EIKTPRAQAKILEVARTGNQPDLRRHAIHMLADIEGEAMLGELLKLYDSEQSNEVRRQ
                     LLHAFSEMKSTQAEDKLFAVARDGGGHADLRRHAIHMIGERAGKRSLDLLRETIESSS
                     AETQVQMQAVHAISERPADESVPLLIKVARTHPNQQVRKRAIHVLGESGDPRALEFFR
                     EVLRSEKE"
     gene            3408..3872
                     /locus_tag="VGB76_07405"
     CDS             3408..3872
                     /locus_tag="VGB76_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098766.1"
                     /translation="MRSKQKFVVALLALFALLCAAPVYGDDGGEQKSKDKRKDKRFEP
                     VVKQNVADYRGRYVGLEDYYLEIRTGTDGQLSIVSHEGGRRATLQNISLTGARLTATR
                     VYQDGTTKEFEATFVNRIMNGDSRFGLMVENPEVKFEGLTFNRIFYRLDSKH"
BASE COUNT          824 a         1304 c         1149 g          597 t
ORIGIN      
        1 tattaacgga aatagagaac agccagcaca cccgcgaagg aaacagtcat ccatatgtca
       61 cacgctttca acttcaaccc gcgccgcctc ctgctgcgcc gcctcccgac gacgcgccgc
      121 gccgccatcg ccctgacgct cgccttcgtc gccgtcgccg cgtgcgtcct gtcagcgcgc
      181 atgcgcgtcg tctacggctc ggaccaagac ctcgccaagc tcaaccgctt cgtgcagacg
      241 ggcaacactc ccgcgatgaa gatgtttcgc gcggggcgcg acctgatcga gaacgaggaa
      301 tgggcgaagg ctgccgagac gtttcgcggc ttcatcaaca cctacccgcg cgacaacgac
      361 gtggacgccg ccctctactg gtacgcctac gcgctcaagc gacagggcag cacgaaagag
      421 gcggccaagg ctctcaagcg tctgatcaaa gatttccagc gttcgggctg gcgcgaagag
      481 gccgacgcga tgctcaccga actcgctccc gcgctcggcc agaccgagat catagataac
      541 gcgctcggca aagagaacga ggaactcaag atcatcgcct tgcagagtct cttcgaatca
      601 agccccgacc gcgcgctcgc tttcgtctcc gagtggttga agcccaactc gacggcgagc
      661 cgccgcatga aagagtccgc cgtctccctg ctcggcgcgc acggcggcaa acaggccatc
      721 ccgctgctcc tcgacatcgc gcgccaccag cccgacgcga agctgcgaca aaccgccatc
      781 catcgtctcg gcgacgcggg cggcgaagcc gtgatggacg acctgatgcg catttacagt
      841 tccgaccccg accccaagat caagcagcag gtgctccacg ccctcgccgg gatggacggc
      901 gcgcgtgcga gcgcgaagct gttggagatc gcgcagaaca acggcgaaga cctcgaactg
      961 cgcaagaccg ccatccaccg tctcggcgag agagacgccg ctttcgacca cctcgcccgt
     1021 ctctacgaca cagaccgctc gctcgaaatt cgcaagcggc tcctcaactc cttctccgag
     1081 tcggacgacc cgcgcgccgc cgccaagctc ctcgaagtcg cgcgcacggg cgaccatgtc
     1141 gaactgcgtc gcagcgcgat tcgtcgtctc ggtgaaaaga acagcgaggc catgctcgac
     1201 gagttgatgc gcatttatca gggggagcag actttggaag tgaagcgcga tattctccac
     1261 gccttctccg acatgaacaa cgcgcgcgcc aagtccaagc tctacgagat cgcgcgcaac
     1321 ccggcggaga acgcagacct acgccgcaac gtcattaacc gcatcgccga acgcaacgac
     1381 ccgcaggccg tcgaactgct catctcgctc tacgatgccg agtcgaactt cgacatcaaa
     1441 cgcaccattc tcaacgcgct cgcccattcg aaccagaagg ccgcgctccg caaactgatg
     1501 caggtcgcgc gcacggacgc ttcgctcgac ctgcgccgtc gcgccatcca gagcatcggc
     1561 gagagcaaag accccgaagc cctgcgcttt ctggaagaca tactcaagcc ctagcgggct
     1621 gccccttgtt cgtcgccggt tgcccttagc ccacgattct cctgtcgggc ggctaaagat
     1681 gaaagacgcg caaccggcga cgggcacgaa acaacttgcg accgacaacc aactgccgac
     1741 cgcccctcga aaggaattca tcatgcgaag atcactatca ttcaattcgt tgttcatctg
     1801 tctagctctg ctcggcggct cggcctgcgc tcacgcgggc aatgcaaagc cgttcgaagc
     1861 ctcggcctcc tctgtcgtcg agacacaggc gtctccgtcc ggcgcggcgc aaacttccaa
     1921 cttcatacag gttgagggcg cagacctgcg cgcgaaactc gacgcggcgg cgagccgcgc
     1981 acgtcaggcg cgcacgcctt actggtcggc ctactcgttc gacgtgcgac cgggcatcgc
     2041 gattgatccg ggcatgggtg agtttcacgg ttcgatgaac acgagcggcg gcacgaccgt
     2101 cttcgtcggc agttccaggg gcatgacggt cgagacgcgc aacctcgccg tctttctcct
     2161 gcgcgatgcg gcgagcggcg gcgcgatcac gcgcatggaa atctacaacc tcgaacgcaa
     2221 acgcgagttc agcggctacc ccgtctactg gcttgggcgc gcgggcaatg aagagagctt
     2281 gaactacctg cgcggcatcg ccgaagccac gcctccgcca gcttcaacca ccagcgcggg
     2341 cttgctcaat caacacgcca cgctcgccat tgccatccac gatgacgcgc gcgtcagccc
     2401 gttgctcaaa cagtttgcgg gcagttcgcg ccacccgaag gtgcgctcaa cggctgtcta
     2461 ctggctgggg cagatcggcg gcgagacgga tttcctcgcc ggtatcgtgc gcaacaacag
     2521 tgagacgaaa gacgtgcggc ggcaggccgc gcacgccatc ggcgagagcc gcgaccgcgc
     2581 cgcgctcacc acgctgcaaa gcctctacga aggcatgacg gagcgcgatg tcaaacgcgg
     2641 catcattcac gccgtcgcgg gcaatgagaa caaggagggc gcgctcgcgt tcctgctcaa
     2701 agtggcgaag accgacgcgg acaaggaatc gcgtcgcacc gccgtccacg ccatcggcga
     2761 cgaggggcgc gattccgtca tcgacgaact gatgagcatc atgtcgagcg atcaggaaca
     2821 ggacgtgcgc cgcgccgtgc tccacgccct cgcagagatc aagacgccgc gcgcacaggc
     2881 gaaaattctg gaagtcgcgc gcacgggcaa tcaaccggac ttgcgccgcc acgccataca
     2941 catgctcgca gacattgaag gcgaagccat gctcggcgag ttgctcaaac tctacgactc
     3001 ggagcagagc aacgaggtgc ggcgtcaact tctccacgcc ttctccgaga tgaagagcac
     3061 gcaggccgag gacaagctct ttgccgtggc gcgcgacggc ggcgggcacg ccgatttgcg
     3121 tcgtcatgcg atccacatga tcggcgagcg ggcgggcaag cgcagcctcg atctgttgcg
     3181 cgagacgatt gagagttcga gcgcggagac gcaggtgcag atgcaggccg tccacgccat
     3241 cagcgaacgc cctgcggacg agtccgtgcc gctgctcatc aaggtcgcgc gcacgcaccc
     3301 gaaccagcag gtgcgcaaac gcgcgatcca cgtgctcggc gaatcgggcg acccgcgcgc
     3361 gctcgaattt ttccgtgaag tgctcagaag cgaaaaggag tagagagatg aggtcgaagc
     3421 aaaaattcgt cgtcgcgttg ctggcactct tcgcgctcct gtgcgccgcg cccgtctacg
     3481 gcgacgacgg cggcgagcag aagtcgaagg ataagcggaa agataaacgc ttcgaaccgg
     3541 tcgtcaaaca gaacgtcgcg gactacagag ggcgttacgt cgggctggaa gattactatc
     3601 ttgagatcag gacgggcacg gacgggcaac tctccatcgt cagccacgag ggcgggcggc
     3661 gcgcgacgct gcaaaacatc agcctcacgg gcgcgcgtct gacggccacg cgcgtctatc
     3721 aggacggcac gacgaaagag ttcgaggcca ctttcgtcaa ccggattatg aacggcgaca
     3781 gccgcttcgg cctcatggtg gaaaatccgg aggtgaagtt cgaaggcttg accttcaacc
     3841 gcatcttcta ccgcctcgac tcgaagcact gacc
//
LOCUS       DASWNS010000219         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_346288, whole genome shotgun sequence.
ACCESSION   DASWNS010000219 DASWNS010000000
VERSION     DASWNS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346288"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VGB76_07410"
     CDS             <1..1248
                     /locus_tag="VGB76_07410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0098767.1"
                     /translation="QVTNRAKFVEKWRETLERENLPEDKLRMREASNRKRAPHVIIFD
                     ERIPTPDRDAGSARMMFILKSLVKWSRPVFVPLYRPHGIEYERLLWKEGIETAHLVEY
                     PRILRTRNFYAAILSRPLVADNLLPRLRRKGGSSLKIVFDMVDAYFIRLAREHEVTRD
                     AHWAEEAGKYREVELRLAGASDLVWCNSSEDKKAVALEVPHVRIEVIPTIHPLHARGK
                     AFEERAHLLFLGNLIHRPNADAVHHLMRDIFPLVKKSLPGVKLYIVGDNVTPEIATYA
                     APDVEVLGYVPDVEPLFQSCRLMVVPLRYGAGVKGKLGESLAYGLPVVTTTIGAEGFG
                     LTDGAEALIADEPQAFASAVVRGYEGKDLWERLAEQGHRHIEKYYTPEVVAEIINSSV
                     QELGLTELERRAASSRDGVETNG"
     gene            1245..2213
                     /locus_tag="VGB76_07415"
     CDS             1245..2213
                     /locus_tag="VGB76_07415"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0098768.1"
                     /translation="MRILITGSRGFVGGSVGRYAVRAGHEVLGVGRATQADVSWPGVY
                     VSADIVSSDLSGVIHDFAPDVVLHAAGTASVGASLTAPLDDLRAALLTWANTLESVRR
                     SGLRPLLLYASSAAVYGNLTELPVREDAPARPISPYGFHKAACELLAHEYAECYSLPV
                     VVCRLFSLFGATQRRLLVWELYRQFAAGNDELVWLQGTGTETRDYLHIDDVAAALLDI
                     AAAQLEATVEHGMEATKADGASRCLVLNVASGEETSVSELARRIGQLVAPQKEIRCRG
                     LERPGDPRAWRADITRLRTLAPYWHPQPLTASLAQCIAAWQAAAHK"
     gene            complement(2314..>3420)
                     /gene="wecB"
                     /locus_tag="VGB76_07420"
     CDS             complement(2314..>3420)
                     /gene="wecB"
                     /locus_tag="VGB76_07420"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEY0098769.1"
                     /translation="STMLKVINVVGARPNFMKVAPIVEAMRRRATEFAPLVVHTGQHY
                     DAQMSAAFFHDLELPEPDVHLGVGSGTHAEQTAAVMQRFEPVVLREQPDWVVVVGDVN
                     STLACALVCAKSGVRVAHVEAGLRSRDRTMPEELNRLVTDQLSDLLLTPSPDADENLL
                     AENIPRERIRFVGNVMIDSLYAQLERANRSRIREELGVGRGTSYGVVTLHRPSNVDER
                     APLERILAALEEIGERLPLIFPAHPRTRRRLEEFGLMERVEARAPRLRLVEPLGYLDF
                     LRLYSAAALVLTDSGGIQEETTALNIPCLTLRENTERPITITHGTNRLVGTDTARITA
                     AAFDALDHPRAECPPLWDGQAADRILNALLEKQL"
BASE COUNT          622 a         1085 c         1101 g          612 t
ORIGIN      
        1 caggtgacga accgcgcgaa gtttgtggag aagtggcggg agacgctgga acgcgaaaat
       61 ttgccggagg acaaattgcg catgcgcgaa gcctcgaacc gcaaacgcgc gccgcacgtc
      121 atcatcttcg acgaacgcat cccgacaccc gaccgcgatg cgggcagcgc gcgcatgatg
      181 ttcatcctga agtcgctcgt gaaatggagt cgtcccgtct ttgtgccgct ctatcgcccg
      241 cacggcatcg agtacgaaag actcctatgg aaggaaggca tcgagacggc gcatctcgtg
      301 gaatacccgc gcatcctgcg gacgcgaaac ttctatgctg cgatcttgag tcgcccgctc
      361 gttgcggata atttgctgcc ccgtctgcgg cgcaaaggcg gctcgtcgct caagatcgtc
      421 ttcgacatgg ttgacgccta tttcatacgg ctcgcacgcg aacatgaggt gacgcgcgac
      481 gcgcactggg cggaagaggc cggaaagtac cgggaggtcg aactgcgcct cgcgggcgcg
      541 agcgatctcg tctggtgcaa ttcgtcggaa gacaagaagg ccgtcgcgct cgaagttcct
      601 catgtgcgga tcgaagtcat cccgacgatt cacccgctcc atgcgcgcgg caaggcgttt
      661 gaggaacgcg cgcacctgct ctttctcggc aacctcatac accgcccgaa cgcggatgcc
      721 gtgcatcacc tgatgcgcga tatttttccg ctcgtgaaaa agtctctgcc cggcgtgaaa
      781 ctctacatcg tcggcgacaa cgtgacgccg gagatcgcta cctacgccgc gcccgatgtt
      841 gaagtgctgg gctacgtgcc ggatgtcgaa ccgctgtttc agagttgtcg tttgatggtc
      901 gtgccgctgc gctacggcgc gggcgtcaaa ggcaaactcg gcgaatcgct cgcttacggt
      961 ctgcccgtcg tgacgacgac gattggcgcg gaaggtttcg gcctgaccga cggcgcggaa
     1021 gcattgatcg cggacgaacc gcaggcgttc gcctcggcgg tggtgcgagg ctacgagggg
     1081 aaagatttgt gggagcgtct ggcggagcag gggcatcgcc acatcgaaaa atattacacg
     1141 ccggaagtgg tggcggaaat catcaactct tcggtgcagg aactcggcct gaccgaactt
     1201 gagaggcggg cggcgagtag ccgcgacggc gtcgagacaa acggttgaga atcttaatca
     1261 cgggaagtcg tggattcgtc ggcgggagcg tcgggcgcta tgccgtgcgc gcggggcatg
     1321 aagtgctcgg cgtcggtcgc gccacgcagg ccgatgtgag ttggccgggc gtttacgtct
     1381 cagccgacat cgtgagttcc gacctctccg gcgtcataca cgacttcgcg cccgatgtcg
     1441 tgctgcatgc ggcgggcacg gcctccgtcg gcgcatcgct cacggcaccg ctcgacgatc
     1501 tgcgcgccgc gctcctgacg tgggcgaaca cgcttgagag cgtgcgccgt tcgggactcc
     1561 gccccttgct gctctacgct tcgagcgcag ccgtctacgg caacctgacg gaacttcccg
     1621 tccgcgaaga tgcgcccgcg cgccccatct cgccctacgg ttttcacaag gccgcctgcg
     1681 aactgctcgc gcacgaatac gcagagtgct actcgctgcc cgtcgtcgtc tgccgtctct
     1741 tctcgctctt cggcgcaacg cagcgtcgtc tgctcgtctg ggagttgtat cgtcaattcg
     1801 cagccgggaa cgacgagctt gtctggctgc aaggcactgg caccgagacg cgcgactacc
     1861 tgcacattga cgacgtagcc gcagccctgc tcgacattgc cgccgcacag ttggaagcga
     1921 cggtggagca cggcatggaa gcgacaaagg cagacggcgc gagtcgctgc ctcgtcttaa
     1981 acgtggcgag cggcgaagag acgagcgtgt cggaactggc gcggcgcatc ggccaactcg
     2041 tcgcgccgca gaaagagatt cgctgccggg gactcgaacg ccccggcgac ccccgcgcct
     2101 ggcgcgcaga catcacacgc ctccgcacgc tcgcccccta ctggcacccg caaccgctca
     2161 ccgcatcact cgcgcagtgc attgcagcgt ggcaggcggc agcgcacaaa tagctcaagc
     2221 caatccaccc gcaggcaaac gcggagcgag acgaattcgt taatccttct gaatccgcct
     2281 cgctccgcgt ttagccgcga acagtctctc tttctacaat tgcttttcga gcagcgcgtt
     2341 gaggatgcgg tcggcggcct gcccgtccca gagcgggggg cactccgcgc gcgggtggtc
     2401 gagtgcgtcg aaggcggcgg cggtgatgcg cgcggtgtct gtgccgacga gtcggttcgt
     2461 gccgtgcgtg atggtgatgg ggcgttcggt gttttcgcgt agggtgaggc aggggatatt
     2521 gagcgcggtc gtctcttcct gtatgccgcc cgaatcggtg agcacgaggg cggcggcgct
     2581 gtagagtcgc agaaaatcga gatagccgag cggctcgacc agacgcaggc gcggcgcgcg
     2641 cgcttccacg cgctccatca gtccgaactc ttcaagcctg cgacgcgtgc gcgggtgcgc
     2701 cgggaagatc aacggcaggc gttcgccgat ctcttcgagg gcggcgagga tgcgctcaag
     2761 cggcgcgcgc tcgtccacat tcgaaggacg atgcagcgtc accaccccat aactcgttcc
     2821 ccgcccgacg cccaactctt cgcgtattcg tgaacgattg gctcgctcaa gttgcgcgta
     2881 gagcgaatcg atcatcacgt tgccgacgaa gcgtatgcgc tcgcgcggga tattttcggc
     2941 gagcagattt tcgtcggcgt cgggcgaggg cgtgagcagg aggtcggaga gttgatccgt
     3001 gacgaggcgg ttcaactctt cgggcatcgt gcggtcgcgg ctgcgcaaac ccgcctcgac
     3061 gtgcgccacg cgcacccccg acttcgcaca gaccagcgcg cacgcgagcg tcgagttgac
     3121 atcgcccacc acgacgaccc aatccggctg ctcacgcagg acgaccggct cgaagcgttg
     3181 catcacggct gccgtctgct ccgcgtgcgt tccagacccc acgccgagat gcacgtctgg
     3241 ctccggcaat tcgagatcgt ggaagaaggc tgcggacatc tgcgcgtcgt agtgctgccc
     3301 cgtatgcacg acgagcggcg caaactccgt cgcgcgccgc cgcatcgcct ctacaatcgg
     3361 cgctaccttc ataaaattcg ggcgcgcgcc caccacgtta atgactttga gcatagtgct
//
LOCUS       DASWNS010000220         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_9892, whole genome shotgun sequence.
ACCESSION   DASWNS010000220 DASWNS010000000
VERSION     DASWNS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9892"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2436
                     /locus_tag="VGB76_07425"
     CDS             <1..2436
                     /locus_tag="VGB76_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0098770.1"
                     /translation="NPFVTRPILGTSQTERFKPTDIRHQFGGAIGGRIIRDKLFFFFT
                     YDQQKRNFPGAAVLSNQAFLNNTSATAACTATPGVNRNCLRGRSAANATFLTDANINQ
                     ATEFISSLLGEVPRSQDQRIIFPKIDWVINGSNNFTASYNRMRSQSPAGIQTQPTTFS
                     GIASFGDDFVEADIFNARLTSTVSPTLLNELRFQWGRDNLFAFSQEPTSGEAAILPAG
                     LARLPNVSLTSGISFGKPTFLERVANPDEKRIQVADTVTSSRGNHTLRFGFDINHVND
                     LLDNLVTEGGSYNYANFNDFIVDYLNARQSGALRTAGVLCATSTRLAGKCYNSPTGYT
                     QSFGPTAFEISTNDYSFFVQDDWRATPRLTVNLGLRYEYQKLPEPQIPNMLPNLSGQL
                     IGPEQTNQIPSDKNNFGPRFGLAYDLTGDGKTSLRGGYGIYYGRIINSTIINAISNTG
                     VAGAQRTFTFNPTDASSPIFPNVVPTAPTGAVVPNIVVFSPGMKSPLIHQADIILERE
                     IGRNTAVSISYLFSRGRNLPTFVDENLPTPIQQSYTFAGGALGGQTITVPFFQRLAAG
                     NGRPDTRFGAITQIRSDIESKYDGLVLQFNRRLTKGLQLQTHYTFSRARDNGQNSQTF
                     TFTQGRLNPFDETVDEEGASNNDVPHRFVFSAVWNPSAPFGLENSRVGRAIFGGFTIA
                     PIFVAQSGNVYTANFSGNVGNNPISGGLTGSGGGSRITAFERNSFRQPKLVNLDLRIS
                     RRFRFTETMNLELLAEGFNVFNRYQATGVNNLAYNLSGSTLTALPAFGQVNAAGNSIY
                     RERQIQFAARFQF"
     gene            2783..2995
                     /locus_tag="VGB76_07430"
     CDS             2783..2995
                     /locus_tag="VGB76_07430"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY0098771.1"
                     /translation="MGLLNNDEVEGKFDQAKGAVKDKVGEATGDRDLEAEGEADRASG
                     NIQEGFGTARRKVGETIEDIGDKISR"
     gene            complement(3148..3648)
                     /locus_tag="VGB76_07435"
     CDS             complement(3148..3648)
                     /locus_tag="VGB76_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098772.1"
                     /translation="MATLVTGLFKTKLAAEAGVDALIKHGYTRDDISVLMSDATKNKE
                     FAIETGTHAVDGAGIGGAVGGTVGAVLAAIAAVGTSIALPGIGLVIAGPIAAALAGAG
                     AGGFTGGVIGALVGAGIPEHRARVYDAGLRSGGILIGVEAKSSEDASTIEKLLTDLGA
                     EHVRQE"
     gene            3848..4543
                     /locus_tag="VGB76_07440"
     CDS             3848..4543
                     /locus_tag="VGB76_07440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0098773.1"
                     /translation="MLKAIIFDIDGTLVDSVDLHARAWQEAFKKFGREVEFEKVRHQI
                     GKGGDQLMPVFFSAEELERFGEELEKYRGELYKRDYLSQVRAFPQVRELFERIRSRGL
                     RIALASSAKKDELKTYKEIARITDLVEEETSADDADKSKPHPDIFEAALAALGDVRAD
                     EAIVIGDTPYDAEAAGKINLRTIGVLCGGFPEAELRAAGCAPIYRDPADLLARYDESP
                     LAQTSSATTSASD"
BASE COUNT          990 a         1580 c         1232 g          840 t
ORIGIN      
        1 aacccgttcg tcacccgccc gatcctcggc acgtcgcaga ccgagcggtt caagccgacg
       61 gacatacgcc atcagttcgg cggcgctatc ggcggacgga tcatccgcga caagctcttc
      121 ttcttcttca cctacgacca gcagaagcgc aacttccccg gcgcggcggt gctgagcaac
      181 caggcgttcc tgaacaacac ttcggccacc gccgcctgca cggcgacccc cggcgtcaac
      241 cgcaactgcc tgcgcggacg ctcggcggcc aatgccacct tcctgacgga cgcgaacatc
      301 aatcaggcca cagagttcat ctccagcctg ctcggcgaag ttccgcgctc gcaggatcag
      361 cgcatcatct tcccgaagat tgactgggtt attaacggca gcaacaactt cactgcctct
      421 tacaaccgca tgcgctcgca gtcgcccgca ggtatccaga cgcagccgac gaccttcagc
      481 ggcatcgcca gcttcggcga cgacttcgtc gaagccgaca tcttcaatgc gcgcctgacc
      541 tccaccgtgt ctccgaccct gctcaacgaa ttgcgcttcc agtggggtcg cgacaacctg
      601 ttcgccttca gccaggagcc gacctccggc gaggccgcca ttctccccgc gggtctggca
      661 cgcctgccca acgtctcgct tacgagcggc atcagcttcg gcaaaccgac gttccttgag
      721 cgcgtcgcca accccgacga gaagcgcatc caggtcgccg acacggtaac ttcgtcgcgc
      781 ggcaaccaca cgctcaggtt cggtttcgac atcaaccacg tcaacgatct gctcgacaac
      841 ctcgtgaccg aaggcggcag ctacaactac gccaacttca acgatttcat cgttgactat
      901 ctcaacgcgc ggcagagcgg cgcgctgcgc acggcgggcg tcctgtgcgc cacttcgacg
      961 aggctcgcgg gcaagtgcta caacagcccg accggctaca cacagtcgtt cggccccacg
     1021 gccttcgaga tcagcaccaa cgactacagc ttcttcgtgc aagacgactg gcgcgcgacg
     1081 ccgcgcctga ccgtcaacct cggcctgcgc tacgagtacc agaagttgcc cgagccgcag
     1141 attccgaaca tgctcccgaa tctgtccggc caactcatcg gacccgagca gacgaaccaa
     1201 attccgtctg acaagaacaa cttcgggccg cgcttcggcc tcgcctacga tctgacgggc
     1261 gacggcaaaa cgtcgcttcg cggcggctat ggcatctact acgggcgcat catcaactcg
     1321 acgatcatca acgccatcag caacacgggc gtcgccgggg cgcagcgcac cttcaccttc
     1381 aacccgacgg atgcctcttc gcccatcttc ccgaacgtgg tgccgacggc tccgaccggc
     1441 gcggtcgtgc cgaacatcgt ggtcttctcg cccggcatga agagtccgct catccatcag
     1501 gcggacatca ttctggagcg cgagatcggg cgcaacaccg ccgtctcgat ctcctacctg
     1561 ttcagccgcg ggcgcaacct cccgaccttc gtggatgaga acctgcccac gccgattcag
     1621 cagagctaca cgttcgcggg cggcgcactc ggcggccaga ccatcaccgt gccgttcttc
     1681 cagcggctcg cagccggtaa cggtcgcccc gacacgcgtt tcggcgcgat cacgcagatt
     1741 cgcagcgata tcgagtcgaa gtacgacggc cttgtgctgc aattcaatcg tcgcctgacg
     1801 aagggtctac agttgcagac ccactacacg ttctcgcgtg cgcgcgataa cggccagaac
     1861 tcgcagacct tcaccttcac gcaaggcagg ctcaacccgt tcgatgagac ggttgacgag
     1921 gaaggcgctt cgaacaacga tgtcccgcac cgcttcgtct tcagcgcggt ctggaatcct
     1981 agcgcgccct tcggtctgga gaattcgaga gttggccgcg cgatcttcgg cggcttcacc
     2041 atcgccccga tcttcgtcgc gcagtcgggc aacgtctata cggcgaactt cagcggaaac
     2101 gtgggcaaca accccatctc cggcggtttg accggatcgg gcgggggttc gcgcatcacg
     2161 gccttcgagc gcaactcttt ccggcaaccc aagctcgtca acctcgacct gcgcatctcg
     2221 cgtcgtttcc gcttcaccga gacgatgaac ctggaactgc tcgccgaggg attcaacgtc
     2281 ttcaaccgtt atcaggcaac gggcgtcaac aatctggcct acaacctgtc cggctctacg
     2341 cttacggcct tgcctgcttt cggtcaggtc aacgcggcgg gcaactcgat ctaccgcgag
     2401 cgtcagattc aattcgccgc acgcttccag ttctaacgcg gacggcaaga gagagtctgc
     2461 actaaccaaa aaagagaggg cgcgtcgtaa gttcgacgcg ccctctcttt ttgtccgaag
     2521 acgaagcgcg cccgccccgt actgtacgga aacggacgat ccgcaaacgc gctctcccgg
     2581 cctccacaaa tgcgcgcaca acctgcgcaa gccgctctcc aaatgggact ttcacatctt
     2641 cgccgtcaac ctcaaacacc cgcgcacgct actggcacgg tcgttgccga gtctcttccg
     2701 agacaaggcg gaaacaagcg tttccgcttt gttccacacg tcaagcggga acaccaattt
     2761 ttcgaggagg cacgatagca ttatgggttt actgaacaac gatgaggtcg aaggaaaatt
     2821 tgatcaggcc aaaggtgcgg tgaaagacaa ggtgggcgaa gccacgggcg accgcgacct
     2881 cgaagccgaa ggcgaggccg accgcgcctc cggaaacatt caggaaggct tcggcaccgc
     2941 ccgccgcaaa gtcggcgaaa ccatcgaaga catcggcgac aaaataagcc ggtaactttt
     3001 aagtgacgac taacgagtga caagcaggaa gaaagtagat cgtgaatcgc agatcgtgaa
     3061 tagagaaaat agagttcttt ctatgtcacg atttacgatt cacgatctac tgttttcatc
     3121 tagtcacttg tcacctgtca ctcgtcacta ctcttgcctg acgtgctccg cgccgaggtc
     3181 tgtgagcagt ttttcaatcg tactggcgtc ttcgctcgac ttggcttcga cgccgatgag
     3241 aatgccgccg ctgcgcaagc ccgcgtcata aactctagca cgatgctccg gaatgcccgc
     3301 gccgacgagc gcgccgatca cgccgcccgt gaatccgccc gcgcccgcgc ccgcgagcgc
     3361 agccgcgatt ggacccgcga taaccagacc gattcccggc agcgcgatgc tcgtgcccac
     3421 cgctgcaatc gccgccagca ccgcgcccac cgtgccgccg accgcgccgc cgatgcctgc
     3481 gccgtcaacc gcgtgcgtcc ccgtctcaat cgcaaactct ttgttcttcg tcgcgtcgga
     3541 catgagcacg ctgatgtcgt cgcgcgtgta gccgtgtttg atgagcgcat cgacgccggc
     3601 ctccgccgca agtttcgttt tgaaaagtcc cgtcacaagt gttgccataa atcctcctca
     3661 gattttccgc tcttaactca aaatgttaat ccgttcaaac aatcaagagc aatctttatg
     3721 ccgactcgtc aaagggcgtt aacgctggct ccgcagcgcg aaccgacgtt ggagaacacg
     3781 acacatcgcc cgccgttggt gtaatgtaaa gcctcacgac acacactcaa ggggagaaac
     3841 agagttaatg ctcaaagcca tcattttcga catcgacggc acgctcgtcg attcggttga
     3901 cctccacgcg cgcgcctggc aggaagcctt caagaagttc gggcgcgagg tcgagttcga
     3961 gaaagtgcgc caccagatcg gcaaaggcgg cgaccagttg atgcccgtct tcttttccgc
     4021 agaagaactc gaacgcttcg gcgaagagtt ggagaagtat cgcggcgaac tttacaagcg
     4081 cgactatctt tcgcaggtgc gcgccttccc gcaggtgcgc gaactcttcg aacgcatccg
     4141 cagcagagga ctccgcatcg cgctcgcctc ttccgccaaa aaagatgaac tcaagactta
     4201 caaagagatc gcgcgcatca cagacctcgt cgaagaagag acttccgcag acgatgccga
     4261 taaatcgaaa ccgcacccgg acatcttcga agccgcgctc gccgcgctcg gcgacgttcg
     4321 tgccgacgaa gccatcgtca tcggcgacac gccctacgat gcggaggccg cgggcaagat
     4381 aaacctgcgc accatcggcg tcctctgcgg cggcttcccc gaagccgaac tgcgcgccgc
     4441 cggatgcgct cccatctacc gcgacccggc tgatctcctc gcacgctacg acgagtcgcc
     4501 gctcgcacag acatcgtcag ccacgaccag cgcgagcgac taaaatgtta tttcccggcg
     4561 accaacaacc gcccgacctt ttaacttctt cgctgcgtct ttgcgcgatt aaatttcacg
     4621 caaaggcgca gagaggcaaa ga
//
LOCUS       DASWNS010000221         5592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_60544, whole genome shotgun sequence.
ACCESSION   DASWNS010000221 DASWNS010000000
VERSION     DASWNS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60544"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /locus_tag="VGB76_07445"
     CDS             <1..1880
                     /locus_tag="VGB76_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0098774.1"
                     /translation="NPAFSPDGQKIAYLKGRGNVNHEEELVSESAFEIHYWDGQTSRY
                     VMDLQSRGTNARMPILSFDPKGERIFFLESPSAPTPPGQPAQIFSALSSIKISGDDYK
                     RHIEARFATEIAPSPDHNWVFFKELHKLYLAPFPQTGKTLKINSTETILPVKSISKNS
                     GDWLTWSPDSKSVQWTLGENFYEQTLEQINRQLAKDEKPTEPRATKINFEFETARPRG
                     VVALTNARIITMRGDEVIERGTILVEDNRIKAVGARVQIPANARRIDMTGKTIAPGLI
                     DVHAHMGYNTLDIIPEKQWPYWANLAYGVTTTHDPSAATQSVFAQSEMVKAGLMVGPR
                     IFSTGYPVYGAESPEKAPVNNLEDARGHLARLKAVGAFSVKSYNQPRRNQRQQIIQAA
                     RELKMMVVPEGGSTYFYNMTHILDGHTGIEHAIPVAPLYKDAVMLFAKSRSGYTPTLV
                     VGYGGVWGENYWYQKSNVWENEKLLRFTPRSTIDARSRRRIMIPEDDFYHFELAKSVR
                     DVVRAGGRAQLGAHGQLQGLGAHWELWMLQQGGLTPLEALRAGTLSGAQYIGLDREVG
                     SIETGKLADLMITDKNPLDQIQNSETISFVMMNGVLYDTRNMDEVYPQARPRGKFFWE
                     R"
     gene            2261..3649
                     /gene="ftsY"
                     /locus_tag="VGB76_07450"
     CDS             2261..3649
                     /gene="ftsY"
                     /locus_tag="VGB76_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY0098775.1"
                     /translation="MAFWRRKKEDRYITLGLNEPRTPAEKAEKEAATATPQTATTPEP
                     PANLSNTQAGASAVAPALPALEPTVTGGTPTPQESRGERASIGATSGNVAREATTPAT
                     TTGGASRTTPEPSKPGVAAQRPVPTRSSFTTSILGLDRSIEELQAEEAALERTFEKRF
                     KNAISATRESLSEKIDTVFQGRKQIDAELLDELEEALIAADIGVPTTLDILDTVRRGI
                     TRKEINDIDALKAAIKNQLLAILRASEAKGVAAETSVPENISPYVMMIVGVNGVGKTT
                     TIGKLAQRIKAEGNDVLICAADTFRAAASDQLAIWAERTGVPLIQQKQGTDPAAVLFD
                     SLKAAKARNSDVLIVDTAGRLHNKSNLMAELEKMKRIAGREVEGAPHETLLVIDAVTG
                     QNGLEQARQFLKVAGVTGIVLTKLDGTAKGGIAVAISKELGLPIRYAGIGEKVDDLVV
                     FNAEHYVDGLFA"
     gene            3633..4502
                     /locus_tag="VGB76_07455"
     CDS             3633..4502
                     /locus_tag="VGB76_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098776.1"
                     /translation="MAYLHKSMDFYTSLIESAGVEKSYVQVYWAWGAHLGEAIHKMKA
                     RAVAQGVDSPLLVEADPYIFSSLPDTAVSENKGEMYYAGERHFFPPTACYRLPDGVVK
                     SCIEGEYELEDIEAGYQLSELDDELIEVSAVVEEKDLLKVYLAVVGALSEIRVFWVKL
                     QDDWEDAGKEEIYVNERLDSLEAIAQFIRSNPLNTIRNGHVTLTTYATAGATNVSISD
                     HKTIDILTYDRLVAGKVCAVLGGYGLNEKENLISISRGFHHWHYRHPEAFNRHDLIDM
                     LHTQGFSYWNPSA"
     gene            4554..>5592
                     /gene="ribD"
                     /locus_tag="VGB76_07460"
     CDS             4554..>5592
                     /gene="ribD"
                     /locus_tag="VGB76_07460"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY0098777.1"
                     /translation="MHLETDNIDGHLMRRALELAARGAGQVSPSPLVGCVVARDDGEV
                     VGEGFYLYERVKHAETLALEQAGARARGATAYVSLEPHAHQGRTPPCTVALIKAGIRR
                     VVAPIEDPNPLVSGRGFTHLREAGIEVEVGLHADEALRLNEKYIHAHAHARPFVHLKL
                     ACSLDGRIATRTGDARWITNEASRARVHRLRHEYDAILIGSRTATADDPLLTDRSLLP
                     RHRPLARILLDDNLRLSPDSQLARTAGESPVIIFTSGTADESAAATLKARGVEIVRAP
                     RGARDLAWVLENLHARSLQSVLVEGGATVAGMFLDARLVAKVSFFIAPLIIGGSDSRA
                     AVAGTGANHVAD"
BASE COUNT         1231 a         1771 c         1628 g          962 t
ORIGIN      
        1 caaacccggc cttctcgccc gacgggcaga agatcgcgta tctcaaaggg cgcggcaacg
       61 tcaaccacga agaggaactc gtgagcgagt cggccttcga gattcactac tgggacggcc
      121 agacgagccg ctacgtgatg gacttgcaaa gtcgcggcac gaacgcgcgc atgccgatcc
      181 tcagtttcga cccgaaaggc gagcgcatct tcttcctcga atcgccctcc gcgcccacgc
      241 cgcccggaca gcccgcacaa atcttctcgg ccttgagcag cattaaaatc tccggcgacg
      301 attacaagcg gcacatcgag gcgcgcttcg ccaccgagat cgcgccctcg cccgatcaca
      361 actgggtctt cttcaaggaa ctgcacaagc tctatctcgc gccgtttccg cagacgggca
      421 agacgctcaa gatcaactcg accgagacga tcctgcccgt caagtcgatc tcgaaaaact
      481 ccggcgactg gctcacctgg tcgcccgaca gcaaatccgt ccagtggacg ctcggcgaaa
      541 atttctacga gcagacgctc gaacagatca atcgccaact cgccaaagac gagaagccca
      601 cagagccgag agccacgaag atcaacttcg aattcgagac ggccaggccg cgcggcgtag
      661 tcgctttaac gaacgcgcgc atcatcacga tgcgcggcga cgaggtgatc gagcgcggca
      721 cgatcctcgt ggaagacaat cgcatcaagg cggtcggcgc gcgcgtccaa atccctgcga
      781 acgcgcgcag gattgatatg acgggtaaga ccatcgcgcc cggcctcata gacgtgcacg
      841 cgcacatggg ctacaacacg ctcgacatca tccccgaaaa gcaatggcct tactgggcaa
      901 acctcgccta cggcgtgacg acgacgcacg acccttcggc ggccacgcag tctgtcttcg
      961 cacagtcgga gatggtcaag gcgggtttga tggtgggacc acgcatcttc tcgacgggct
     1021 atccggtcta cggcgcggag agtcctgaga aagctccggt caacaatctg gaagatgcgc
     1081 gcgggcatct cgcgcggctc aaggcggtcg gcgcgttctc cgtcaagagc tacaaccagc
     1141 cgcgccgcaa tcaacgccag cagatcatac aggccgcgcg cgaactcaag atgatggtcg
     1201 tccccgaagg cggctccacc tacttctaca acatgacgca catcctcgac ggccacacag
     1261 gcatcgagca cgccatcccc gtcgcaccgc tctacaaaga cgccgtgatg ttgtttgcca
     1321 agagtcgttc gggctacacg cccacgctcg tcgtcggcta cggcggcgtc tggggcgaaa
     1381 attactggta tcaaaaatcg aacgtctggg agaacgaaaa gcttctgcgc ttcacgccgc
     1441 gctcgaccat tgacgcgcgc tcgcgtcgcc gcatcatgat tcccgaagac gacttctatc
     1501 atttcgagtt ggcgaagtcc gtgcgcgacg ttgtgcgcgc gggcggtcgt gcccaactcg
     1561 gcgcgcacgg ccaactgcaa ggactcggcg cgcactggga actctggatg ctccaacagg
     1621 gcggactcac gccgctcgaa gccctgcgcg cgggcacgct ctccggcgcg cagtacatcg
     1681 gcctcgaccg cgaagtcggc tcaatcgaga cgggcaaact cgccgacctg atgatcacgg
     1741 acaagaaccc gctcgaccag attcaaaact ccgagacgat cagcttcgtc atgatgaacg
     1801 gcgtgctcta cgacacgcgc aacatggacg aggtctaccc gcaggcgcga ccgcgcggca
     1861 agttcttctg ggagcgttga aggctttaac ttaaggacga acgacgaacg cgtaacgatg
     1921 aactgaaagc aaccgtcttt cagttcatcg ttacgcgttc ctgcttcatc gtgccacgcg
     1981 tcggcggggc tatccgtcaa ttgctcaagg caacaagggc gttgacgcat ctgttaaatc
     2041 tccttagacg gaagtgtgaa ggcgcgtacg ttttcggagt cgggcgattc ggctggtcga
     2101 acgggcggaa aatatcataa gcgcgcggcg gcaactaaca atttaacgct ccgcgaacct
     2161 gcctgcggtg gtcgcgcgtt ttgtcgctgc gcgcccgttg cagttataat cgacgacttt
     2221 caattcgacg aaatttttag caagggcact caaagttaat atggcattct ggcgacgcaa
     2281 aaaagaagac cgctacatca cgctcggact caacgaaccg cgcacccccg cagagaaagc
     2341 agagaaagag gcggcaacgg caacccccca aacggcgacg acgcccgaac cgcccgccaa
     2401 tctttccaac acgcaggcgg gcgcgtctgc cgtcgctccc gcgctgcccg cgcttgagcc
     2461 gaccgtgacg ggcggcacgc cgacgccgca agagagcagg ggcgaaaggg cgtctatcgg
     2521 ggcgaccagc ggaaacgtcg cacgcgaagc cacgactccg gcgacaacga cgggcggcgc
     2581 atcccgcacg acgcccgaac cgtcgaagcc cggcgtcgcc gcgcaacgac ccgtccccac
     2641 gcgttcgagt tttaccacct ccattctcgg cctcgaccgc tccatcgaag agttgcaggc
     2701 cgaagaggcc gcgctcgaac gcaccttcga gaagcgtttc aaaaacgcca tctccgccac
     2761 gcgcgagtct ctgtcggaaa agatcgacac cgtctttcaa gggcgcaaac agatcgacgc
     2821 cgagttgctc gacgaactcg aagaggctct catcgccgca gacatcggcg tgccgacgac
     2881 gctcgacatc ctcgatacgg tgaggcgcgg catcacgcgc aaagagatca acgacatcga
     2941 cgcgctcaag gccgcgatca aaaatcaatt gctcgccatc ctgcgcgcgt cggaggcaaa
     3001 gggcgtcgcc gccgagacga gcgtgccgga aaacatctcg ccttacgtga tgatgatcgt
     3061 cggcgtcaac ggcgtcggca agacgaccac catcggcaaa ctcgcgcagc gcatcaaggc
     3121 cgaaggcaac gacgtgctca tctgcgcggc tgacaccttc cgcgccgccg cttccgatca
     3181 actcgccatc tgggctgaac gcacgggcgt gccgctcatc cagcagaagc agggaaccga
     3241 ccccgccgcc gtcctcttcg attcgctcaa agccgcgaag gcgcgcaact ccgacgtgct
     3301 catcgtggac accgccgggc gtctgcacaa caaatcgaac ctgatggcgg aactggaaaa
     3361 gatgaaacgg atcgccgggc gcgaggtgga aggtgcgccg cacgagacgc tgctcgtgat
     3421 cgacgccgtg accggccaga acggactcga acaggcgcgc cagttcttga aagtcgcggg
     3481 cgtcaccggc atcgtcctga cgaaactcga cggcacggcc aaaggcggca tcgccgtcgc
     3541 catctccaaa gaactcggcc tgccgatccg ctacgcaggc atcggcgaaa aggttgacga
     3601 cctcgtcgtc ttcaacgccg agcactacgt cgatggctta tttgcataag tctatggact
     3661 tttacacttc attgatcgag agcgcgggcg tggagaaatc ctatgtgcag gtctactggg
     3721 cttggggggc gcatctcggc gaagcaatac ataagatgaa agcgcgcgcc gtggctcagg
     3781 gagttgattc tcctctcctg gtagaagccg atccttacat cttcagcagc ttgccggata
     3841 ctgccgtgtc cgaaaataaa ggggagatgt actatgccgg ggaacgccat ttctttccgc
     3901 cgacagcttg ctacaggctt ccagacggag ttgttaaatc atgtatcgaa ggtgaatacg
     3961 agcttgagga catagaagcc gggtatcaat taagtgagtt agacgatgag ttgatcgaag
     4021 tgtcagccgt cgttgaagag aaagacctgt tgaaggttta tctggccgta gtgggcgctc
     4081 tttccgagat tcgcgtgttt tgggtgaaac tccaggatga ttgggaggat gccggaaaag
     4141 aagagatata cgtgaacgag cggctggatt cgcttgaggc aatcgcgcaa tttatccgct
     4201 ccaaccctct taatacgata cggaatggtc atgtcacgct cacgacgtat gccaccgccg
     4261 gcgcgacgaa tgtgagcatc agcgaccaca aaacaataga tatactcacg tatgacaggc
     4321 ttgttgccgg taaagtttgc gcggtgttag gtggttatgg gttgaatgaa aaagagaact
     4381 taatatctat cagccgtggt tttcatcact ggcattacag gcaccccgaa gcgtttaacc
     4441 ggcacgacct gattgacatg cttcatacgc aagggttcag ttattggaat ccctcggcct
     4501 gatacttcgt ggcacataga tcccccgaac agtgtgtgac atcaaactct gatttgcatc
     4561 ttgaaactga caacatagac ggccacttga tgcgtcgtgc gctggaactc gcggcgcgtg
     4621 gcgcggggca ggttagcccg agtccgctcg tcggctgcgt cgtcgcgcgc gacgacggcg
     4681 aagtggtcgg cgaaggtttc tacctgtacg agcgcgtcaa acacgcggag acgcttgcgc
     4741 tcgaacaggc gggcgcgcgc gcgcgcggcg cgaccgctta cgtttcgctc gaaccgcacg
     4801 cgcatcaggg gcgcacgccg ccctgcacgg tcgcgctcat caaggctggc atccggcgcg
     4861 tcgtcgcgcc catcgaagac ccgaacccgc tcgtgtcggg gcgcgggttc acgcacctgc
     4921 gcgaagccgg gatcgaagtg gaggtcggcc tccacgcgga tgaagcgttg cgcctcaacg
     4981 aaaagtatat ccacgcccac gcccacgcgc gccccttcgt gcatctcaaa ctcgcctgct
     5041 cgctcgacgg gcgcatcgcc acgcgcaccg gcgacgcgcg ctggatcacg aacgaagcat
     5101 cgcgcgcacg cgtccacagg ctgcgccatg aatacgatgc gatcctcatc ggttcacgca
     5161 cggccacagc agacgaccca ctgctcacag accgcagcct tctgccgcgt catcgcccgc
     5221 tcgcgcgcat cctgctcgac gacaatttgc gcctctcgcc cgattcgcaa ctcgcgcgca
     5281 cggcgggcga gtctccggtc atcatcttta cttccggcac agccgacgag agcgcggcgg
     5341 cgactttgaa ggcgcgcggc gtagagatcg tgcgtgcgcc gcgcggcgca cgcgatctcg
     5401 cgtgggtgct cgaaaactta catgcccgtt cgctgcaaag cgtgctcgtc gaaggcggcg
     5461 cgacggttgc cgggatgttc ctcgatgcgc gcctcgtcgc aaaagtgagc ttcttcatcg
     5521 cgccgctcat catcggcggg agcgattcac gcgccgccgt cgcaggcaca ggcgcaaacc
     5581 acgtcgccga tg
//
LOCUS       DASWNS010000222        42835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_242749, whole genome shotgun sequence.
ACCESSION   DASWNS010000222 DASWNS010000000
VERSION     DASWNS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 42835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42835
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242749"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VGB76_07465"
     CDS             complement(<1..388)
                     /locus_tag="VGB76_07465"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0098778.1"
                     /translation="MTETLRTADDALGSRLDEIRARIAACARRAGRTSGDALTLIAVS
                     KTHPAERVIEAMRAGVSDFGENRVQEAGEKIEIVSRADLDRSASRPRWHLIGHLQSNK
                     ARRAVKLFDLIHTVDSVELIERLERTC"
     gene            578..661
                     /locus_tag="VGB76_07470"
     tRNA            578..661
                     /locus_tag="VGB76_07470"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Leu,seq:cag)
     gene            complement(752..1189)
                     /locus_tag="VGB76_07475"
     CDS             complement(752..1189)
                     /locus_tag="VGB76_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098779.1"
                     /translation="MSTTNEANKLELAYSIFCDDVRLEVGNKLSLIGVFQNMLVQQLP
                     VALIKFAVVNHWRGEGRYLSEVRILSPDKKQPIVVSEPAPFVIAQGGHTDNVSFFVNI
                     TFHTPGEYWVQTLINSTLFSEQPLLIMDARSAQTPESASESIN"
     gene            complement(1244..3283)
                     /gene="ligA"
                     /locus_tag="VGB76_07480"
     CDS             complement(1244..3283)
                     /gene="ligA"
                     /locus_tag="VGB76_07480"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY0098780.1"
                     /translation="MAKSSTRTANLTQEIAQLRDELRHHEELYYVYDNPEISDADYDA
                     LMSRLQALEAEHPELATPDSPTQRVGGRPVEEFAEYVHRRPMLSLDNSYNIEDLRAFD
                     ERIKRLADGRRLEYVAELKIDGLSISLHYERGVLVRGVTRGDGRRGEDVTQNVRTIRS
                     IPLRFKAGGALDLPEIEVRGEAYLSRRTFERINAEREQAEEPRFANPRNAAAGTIRQL
                     DPKIVSARRLDLFAYDCITGDRKAFATHWEGLEWLERAGFSVSHRRLCPSIEEVIEFC
                     NEMEARRDELEYEIDGVVVKVNSAALQDEFGATAKSPRWAIAYKYPARQATTQVLDIG
                     VQVGRTGALTPVAHLEPVLVAGSTVARATLHNEDEIKRLGVRIGDWVLIEKSGDVIPK
                     VIKVVESRRTGTEKRFKMPKACPVCGGVVSRPEGEVVARCVAADCPAQLKARLLHYAS
                     RRAMRIEGLGDALADQFVQKRLVRDVAGLYDLTQEQVAELERMAEKSASNLLAQIEAS
                     KTRDLPQLVFGLGIRHVGERTAAILARQFRTLEALGAASVEELDAVPEIGLTVAESVH
                     DWFADEGNQALCQRLRDKGVRTEMEAGTAAGGVRDESFAGMLFVLTGKLETLTRDEAA
                     ALIEARGGRVTSSVSKKTNYVVAGEEAGSKLDKAQTLGVKVIDEQGFRALLATND"
     gene            complement(3300..4724)
                     /locus_tag="VGB76_07485"
     CDS             complement(3300..4724)
                     /locus_tag="VGB76_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0098781.1"
                     /translation="MPFSRFDRYVERHANAFTERLQALCRMPSVAARGTGMRAMAETV
                     EQSMQRIGGGTRSFKLGSGYPIIYGEFGAGSHCYIAYSHYDVQPVGQLNEWSSGPFAA
                     AVRDGKLYARGAANSKGDLVARLAAIEAYQKTFGKLPLCLRFIVEGEDGLGSPSLYRF
                     TAEHPEMLRADGCIWDDGYKDTKERLLLSLGFKGITFLELRAYGARTDLHSKWGGIVP
                     NPAWNLVQALSTIVSPKGVITIDGFSSHVAPIAEEDMKSLRQLELDEAGLKSEFKINH
                     WVRGLTGRALAKELIFGPTCTICGIHTGHTEAGAKTVLPGMAVARLDFRLVPDLSPAL
                     VVGLLREHLDTRGFQDIEIIEIASAPFAKASIKSSVARAAIESATEIYGAQPIVYPLD
                     PASGPVGAVCGVNLPPTPIVSFGVSYAGSNPHAPDENIRLEDFVQGIKYFGRLIHRLA
                     QVAREDQRETSTQESFVQLKQAKS"
     gene            complement(4878..5675)
                     /locus_tag="VGB76_07490"
     CDS             complement(4878..5675)
                     /locus_tag="VGB76_07490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0098782.1"
                     /translation="MSGHTGKIVGARGDEEVVIRECTTIEEFDTCVRLQREVFHMPDL
                     ELSPRRHLIVSRLAGGWILGAFAAASGELVGFVHHLVAVRGEEIIGYSHMMAVSAAYQ
                     NRGLGARLKWAQRERALAEGRNFIRWTFEPMQARNAHFNLNRLGVVIRSYGVNFYGWD
                     AGTEAFADGKQFNIDSDRLIAQWELRTPRVEQIARGVRAAQASPPAATVEIPPDWNSL
                     RREDGLTAQRELLRVRSEFQAHLDAGLVCAGFERDGARPCYLFFSDE"
     gene            complement(5732..6202)
                     /locus_tag="VGB76_07495"
     CDS             complement(5732..6202)
                     /locus_tag="VGB76_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098783.1"
                     /translation="MHYDFELAGRRVRLWQRTGESYEHVLMKALGYAMFAVEFPALEI
                     EQRVGLRYKPDLVARGGGREFEAPFAFWGECGQTAMRKTHWLLKHAGVARLVLFKIIS
                     HPNALAEELRAAIDERYREPERLVLINFRSDIVARTDERRIARVPESWYTRIVV"
     gene            complement(6292..7659)
                     /locus_tag="VGB76_07500"
     CDS             complement(6292..7659)
                     /locus_tag="VGB76_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY0098784.1"
                     /translation="MNAQVKKSLQPFSLQDESEAASEELSALGAEVVDIFYTQIFAPD
                     EYATEAAVCAAFSELLLRHWNLCCLITFLRDADGRLRESAIHTHPHMDEAQGRRVGHL
                     LSNGVEESGREWQVWLDKEANDEGAAESAVRALLAETGIRAALALPIYARGTLIGSIV
                     AISSHPERLRAAANGLRFIAAPIVIALGNARRVAAMREQREQIEQLVAELRERSSALE
                     AANLELQRVARYRSLFLARMSHELRTPLTSMLGFSEILLEHEHLTPPQQRYCEKIQNS
                     GLQLKTSLKQLVDLSRLEAGQTELFLHEFSVRETLRETCGAVVRLAQKKDVKLDCTTA
                     PDCGTIVSDEGKLRQVLYNFFAHAIGRSPSGGLVSINAFKRTPAELCIEIHDEGDPPA
                     DAAHIFDVIEIDAPNERGTNMNELGLVIAQRLVALLGGRIVPNTDAPKGLTVCLELPA
                     YPTKE"
     gene            7929..9443
                     /locus_tag="VGB76_07505"
     CDS             7929..9443
                     /locus_tag="VGB76_07505"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY0098785.1"
                     /translation="MRERPLAELIREIIAAKLSGALRLARERVQAVVYAEAGQIVFAR
                     SNLRVHRLAVCLNRWGVLAEDKLSAQVTEFMSDAEASASLVASGALTADALAKLLARQ
                     ATDVLRPPLLWTEGEWSFDPRARLVEDVRCKLELHQLLVEAARQLPAEFVAARLADDA
                     EIISPVTELPAQLQLNAPEGFVLSRVDAPLALGELLAISGLPETQARQCVYALALGGF
                     LARSGWPVALSPAQAPAQAAVADKAVAAGVAKPDVVEDKHAPASGAQNAEATPNPRAE
                     IDALFALVYDADHYRILDVGRSADGAAIKRSYYALARRFHPDRFQRDADVSLRTQIEA
                     AFMKITQAYETLRDPRARAAYELKLGTQIDSSGASSKPSAGSSANTHLTREQRAAESF
                     TQGVAALKLNNLSSAITLLGEATRLAPQQSRYHAFYGSALARDVRTRHQAEAELQTAI
                     KLDGNDPAYHVMLAELLRVLGQSLRAERELERALSLDPNHDAARRLLDQLRKRK"
     gene            9755..10636
                     /locus_tag="VGB76_07510"
     CDS             9755..10636
                     /locus_tag="VGB76_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0098786.1"
                     /translation="MASLQTKLTNEDGIAREAFTRLARAADEFEGYAHPHAERIAALC
                     GELAKLFGLGRADRASLHLAALAHDLGELAMKRDYIRRAAPLTFEERLDLARHPVIGE
                     QEAARAGADRGAQLLVRWHQEWWNGAGYPDALSREQIPLAARLLRVADAYAALTDARP
                     FRPAFTEEEARRHLSEWAGLEFDPNVVRAFLTLQNLPALRSFARRDEDAAATATHDAA
                     TQADEPRADAPAVDAPALNAQGWNIGGHIPEAGTNPYVQRAEPNAESRTPAPEQRFDT
                     DQKTDSLRDYDSLRKAD"
     gene            10667..11608
                     /locus_tag="VGB76_07515"
     CDS             10667..11608
                     /locus_tag="VGB76_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098787.1"
                     /translation="MPDYSNSTPRGWLAFELSVLRRLRFRSLINPFAGEPDLETHVKR
                     WGVRVAANDAARWSWIKGIARVENNGERLTGDDLEVLLEDVYVPRYRLRNAALRRWFS
                     ETDAWWFDNLRENAERLEGEIKQSLALNLGMLVGDYALSFDEETRELRQPLSRVLRRL
                     HETQPPPFDNGQPNTSTNKEARQFVAEQRGDLLFLRLPAPARSSSGGAQGREGLPAWR
                     EEWVRGGSEFWDEFEASRAGRLGGRVETKGQHLRLIEELLEAAAHLKSWAIAHTEGGY
                     TTTEELVETIRRVRKVETIYTKDFSELLGARATIITT"
     gene            complement(12594..12986)
                     /locus_tag="VGB76_07520"
     CDS             complement(12594..12986)
                     /locus_tag="VGB76_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY0098788.1"
                     /translation="MMTNDELIQKAQAVVNPRKIAHGLFVGDVGCALLTEKGNLHVGV
                     CIDAVAGIGFCAEHSAIASMVTHGEQSIRKIVAVLGDGTILPPCGRCRELMHQIDESN
                     MDHTEVILGHAKAVQLRDLLPHPWDGVF"
     gene            complement(13116..13574)
                     /locus_tag="VGB76_07525"
     CDS             complement(13116..13574)
                     /locus_tag="VGB76_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEY0098789.1"
                     /translation="MRLLLSALICLCCVSGVFAQTSGKEKKVRQAVQAFYDAFSSDDF
                     GRAAEYTTEDWNHINPAGGWTRGREAVLKEVKEVHSTFLKGVSDTPEEMSVRFATPAV
                     AVVTVPSRVSTYTTPDGIKHENERQIRTFVVVKRGGRWLIMQDHNTVRGR"
     gene            13970..14857
                     /locus_tag="VGB76_07530"
     CDS             13970..14857
                     /locus_tag="VGB76_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0098790.1"
                     /translation="MDWSDRKHDICPIDLATGETQASILSHSPQTIDEWATALRARFG
                     GRQLALCLEQARGPLIYALMKYDFLTLYPVNPSTLARYRQAFSPSRHKDDAPDAAYLA
                     ELLLHHCEHLRAWRPDDEQTRTLRYLVEHRRRLVSDRTRLSNRMTSRLKCYFPQVLDW
                     FPDIRTDLVCNFLLRWPALEALRRVRRETLVKFFQEHHSLRRETIQKRVEAIKQSPPL
                     TTDRAVLSSSVVMSKALAAQMKATLAASKEFDTEIAVHLFDASRLYALQAPARGGSRL
                     CFPPSRRLRQRPGAMAISR"
     gene            complement(15321..15740)
                     /locus_tag="VGB76_07535"
     CDS             complement(15321..15740)
                     /locus_tag="VGB76_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098791.1"
                     /translation="MQETSSDCFIEVWRDVNFKGEVIRIYGPAEHPNLRFVQGDWGDD
                     IGSLRVGPNAFVLAYRDQNFRDELVTFGPNDEVADLRELKFHDDIDSVKVIDSLKIFD
                     CLRYNSKGGEEVAGANSAKGDAGGGKSKRKRHKGKRG"
     gene            complement(15836..17740)
                     /locus_tag="VGB76_07540"
     CDS             complement(15836..17740)
                     /locus_tag="VGB76_07540"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY0098792.1"
                     /translation="MQTFAQIPTATQQNTSAESTAPGRAQFAQNRELNSSLHLQGTSG
                     TQAGRRPLQAKPEGLGAVSDSTGIGRPGHDFSRIPLYPKARAEDIHRVARNGTAGNAA
                     PLPYLNRIQQSFGPGHHLTQVRAYVGSRAAAAARNLEAEAYTVGDSVAFGTEPSLRTA
                     AHEAAHVVQQRHGVRLERGVGRSGDAYERNADAIADRVVRGESARELLDGFPPTGAGS
                     SSSAVQRQPKPEGQVDLFDEEVMERVAGRERDDNEGDTGGGEKANAAARQDFDSLPGP
                     LKEVLAKSFDWPDVFWAGLETRQKISLSEAYNRLSALGLWKHVAKIRGEHDKPERKAY
                     GGRAIVAGSAGGIGFESHNATALVIGLFESGKFGLDPWWAALLHRGQDSLREFNTEDG
                     SLHISVGPGNHFDTHIDQVGAVNKPQDNKGKFDPARSLKHHTREVWPEFIRKGTGIPG
                     VIVGIGPNLSDIRNEPGPYDDNVPPTPSKGEDDILASVNLVAHPIPKEKPRPVPHAGP
                     EAGTARVDREVMARAVAAGAAAATEYDLMPNPVGSDPDDFADPATIASSLAGRITKAA
                     RDGQMTVAINLGPGYSKATPAERKSIGEAIGRIGVAVAGELPQETHGVRSLRVLFGES
                     KSVEYVSLRR"
     gene            18020..18217
                     /locus_tag="VGB76_07545"
                     /pseudo
     CDS             18020..18217
                     /locus_tag="VGB76_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(18403..18930)
                     /locus_tag="VGB76_07550"
     CDS             complement(18403..18930)
                     /locus_tag="VGB76_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY0098793.1"
                     /translation="MRNTFIVVPVFGGTDAGKPESLKIEIREKNGFRGVFAKEEIAMN
                     SVIKLEGVITSHPTRHSIQLGEDKHLSVPPDQEVTQNQNFFWKYLNHSCQPNSEINTA
                     SVTFRPLRKIGQGEECTFNYLTTEYEMAAPFTCNCGAANCFGLIRGYKYLSAEQREEL
                     TAHARTVIVMPEPSS"
     gene            complement(19327..19605)
                     /locus_tag="VGB76_07555"
     CDS             complement(19327..19605)
                     /locus_tag="VGB76_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098794.1"
                     /translation="MKARKTNRREFLGLTGASLVGLAMTAGALSLPGSLAAPVVAAER
                     KPPIEEWLLGTGQPSLSPGIYNNALEVITRNQQTGGKSKTSKGGRSRK"
     gene            complement(19631..20023)
                     /locus_tag="VGB76_07560"
     CDS             complement(19631..20023)
                     /locus_tag="VGB76_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098795.1"
                     /translation="MGGRNNKADRQAKVTLDIFSGRKNPTWSLTEEQTGNLLVIVKGL
                     PAADPCDFFDGLGYRGFQVNMTSPVAGKRSAITVYRGRVRYDDGRQVKYLTDKDRRIE
                     QLLLKYGSPHLTPGLYKTVEREVRPTAE"
     gene            complement(20128..21135)
                     /locus_tag="VGB76_07565"
     CDS             complement(20128..21135)
                     /locus_tag="VGB76_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098796.1"
                     /translation="MITIELDIFSGRPNPLWTLTNKEENELFSRVMADKDMSAPPRKV
                     GHLGYRGFGIKVGGERANRWHAAGIPPSFFISAGHERGGETETFLLNTANAGQTAADE
                     VLKVAQEYIDAANYAWTQRWIRAEYAENGPPLTPANRHEPLIDGTIIVPDPDGRGTPS
                     SEEGQINDKAASDSMSALACGAFSYTSDTNFTYWNSDPYVMQNNNCYNFAANYRSDTF
                     AQPGRKANVEWYDLACGNSPGQIGYAASYDGFNLNCWTSTQYYAALVIWPYTSDPLSG
                     DFHWYRKCANGHWCHKPGKTSARNYDSSGYWITDPYTCDRGPYTIFCSYRYFPTNYTV
                     R"
     gene            complement(21606..22055)
                     /locus_tag="VGB76_07570"
     CDS             complement(21606..22055)
                     /locus_tag="VGB76_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098797.1"
                     /translation="MAEACTEGQPLQAVLTLGAHLHQPVTVAQEPQYLPTFKRRQMHL
                     RELLSQPQVEQQLGIATVCLVSAACATANPGRITDEDLMTKTLQQFDEPSAVAAGFQA
                     DDHVTCGLSVEGTNIIPVVMPFAALDEAVDCVAVTNSLLTSVQSTPQ"
     gene            22078..22638
                     /locus_tag="VGB76_07575"
     CDS             22078..22638
                     /locus_tag="VGB76_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0098798.1"
                     /translation="MGRERFLELPMSAAMARQRSEWLAFVAQFDERIHELDGWFTGQA
                     RQDERVLRLQTHPGIGLLTSLALVYGLEPMSRFAGGRHVAAYVGLEPMEYSSGGKQKF
                     GFMSKGGSRLLRYLLVEAAQTAVRRDKQLKRFYLRLCNERGAQKAKVAAARKLLIRSY
                     ILLRDGIDYAEFLARGVEARPARLAT"
     gene            complement(22775..23489)
                     /locus_tag="VGB76_07580"
                     /pseudo
     CDS             complement(22775..23489)
                     /locus_tag="VGB76_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(23887..24708)
                     /locus_tag="VGB76_07585"
     CDS             complement(23887..24708)
                     /locus_tag="VGB76_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098799.1"
                     /translation="MSRFARLVNAAPPAKTEKEPSRPEESQPLRSSQPQESSRPQESS
                     QLSEQPRGGYDSSQLPQSSQLPQSREANPTQPPDSSQPLQSSQLQESRVNLLASLPDA
                     KGHMETPFQLLDHLFRHLDPFEQAAYMQLCRLSWGWKQNRCRVSNPRLAERANMSLAQ
                     VKKVVAKLIAKGLIEKTGNIQGFGKEQGVEYIVHAPSWQLSRSSQLQQSRQPHKSSQL
                     QQSTNIDKNINIDQKGESASPNLLNCPDCLGTGFFYPEGKAGGVKRCNHERAKGN"
     gene            complement(24708..25511)
                     /locus_tag="VGB76_07590"
     CDS             complement(24708..25511)
                     /locus_tag="VGB76_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0098800.1"
                     /translation="MTSAPSIDDYTPFSISPNPNTLHITPGLKAVLAKVRFTVNKRQG
                     LCALLGDVGLGKSTLLRYLFGEYAANENCLTTLVPTPKFPSPFAFLKYMATDLGVDGK
                     RSLTQQRDELERWLIDRYEAGKNVVIFVDEAQVLDNAQLELVRGLLNFETHTHKLVQI
                     VLAGQLELREKLQSEKNRALYSRISTYSVLDPLTLEETGGMLAHRCKHESINNPFTTE
                     AVEKLYVVTKGIPRSVLKVAALAYEMMLMEGARTIDPELISDTVPEVAV"
     gene            complement(26128..26658)
                     /locus_tag="VGB76_07595"
     CDS             complement(26128..26658)
                     /locus_tag="VGB76_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0098801.1"
                     /translation="MSQDVEILYGDPEKVGEPFVMRIRELAGGVIPPHKHPVDEHITV
                     VQGTLYFGVGDKFDRAALKEMKAGSYAFIPKGSTMFGYTPEAAIVQVQGVGPFHIHWR
                     AGTNWRDSLKTLDSPDATTIFKFRKGERVTTKRGRGRIRQGYDSGEIIGYEIDGEDGS
                     LFMAEEGEIKRLENRR"
     gene            27124..28131
                     /locus_tag="VGB76_07600"
     CDS             27124..28131
                     /locus_tag="VGB76_07600"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HEY0098802.1"
                     /translation="MSAFAQGSKPTVNRDPEKVKFVTSDIDNFWRAFDLAAKEIDREK
                     RIAIFQKEYLDQGSAGLQDFVRLRIKNAKSLVETIERLPKFYASVRHSTSRVAQLEKP
                     MRRSFRKFKQLYPDAAFPDVYFVIGIANTGGTASGSGLLIGAELYGLTPGTPREEFPA
                     WFKTLLPDAGDEKELLRLVAKALDTALKPVEKLPFTVAHESCHFNQNYPKQATLLEKS
                     IQEGACDLIAELTAGQVINPTLKVYGDRNEARLWRDFQAEMSGTDLSKWMYNSFRIKD
                     RPGDLGYYMGYKISQAYYRNAKDKRQAIRDILNIKDFQGFFQRSRYDEKFAAHGAKAH
                     P"
     gene            28338..29108
                     /locus_tag="VGB76_07605"
     CDS             28338..29108
                     /locus_tag="VGB76_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0098803.1"
                     /translation="MRHQLTTRLKLYSCIALLIALCGAGSVRAQQTAVTISGRVSEES
                     TGQGIDSVAVVALGNQTGTRVAITDAQGNYRLPFGANTNIKLRAYKTGFIFNPASVEL
                     ISPGGQPINGARTFDFTGTRLPFPILIFALPPILLTEDESLNALALDAVLHTRDPFPL
                     VNDHYFGADSRTRVKLFLVDLDLYSGETLSIISVQAQDEQRRAYVLPVEDLRKVPNFP
                     WLSQLTIRLPGELAGVTDITLSVSARGQASNVTRLRLR"
     gene            29301..30758
                     /locus_tag="VGB76_07610"
     CDS             29301..30758
                     /locus_tag="VGB76_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY0098804.1"
                     /translation="MFSSRLRVVLFVLLLPGTLFAQEGIPFFTTDFPPEEFAQRRAKI
                     YDEMGSGKIAVVQGAPSPAGFTRFRQSNEFYYLCGVESPHAYLLLDSTRRSASLYLPH
                     RNEGRESVEGKVLSAEDAEMIKRLSGVEAVYGSELLAEHLSRYARSGQTRTLLTPFSP
                     AEGLATSRDGAIRAVADIASDPWDASPSREGRFAEMLRTRFPQFDVQDLSPALDKLRL
                     IKSPREIALISKATRLSSLALLEAMRSTAPGVSEYELDAVAKYVYYRNGAQGDAYYSL
                     VASGRNAWYPHYNSGKRKMLDGDFLLMDYAPDVGYYMSDVTRMWPVNGKFNAWQRELY
                     GFYLDCYQAILKHIRPGVTAAVIKQDALKDMEQALARTRFSKPTYERAAREFVEGYRR
                     SAANPRASLGHWVGMATHDVGQDSGPLRAGMVFTIEPALRVPEEKIYIRLEDVIVITD
                     KGADILTNWLPMDIPAIEKTMSEEGLLQRYPRDRQ"
     gene            31050..31922
                     /locus_tag="VGB76_07615"
     CDS             31050..31922
                     /locus_tag="VGB76_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEY0098805.1"
                     /translation="MFNWSMIKTTLLLGGLTGLLVMMGGLIGGGYGAMLGFVVALAMN
                     FGSYWFSDRIALKMAGAREVSYREAPELHDMVARLAQATGLPKPRVAIVESDAPNAFA
                     TGRNPQHGLVAVTTGILRLLDQRELAAVISHELGHIRNRDVLVSTVAATFAGAITMIA
                     QIGQFAVLFGGASDEDEDGGGNILGALLMIFLAPLAATIIQLAISRSREFGADRTGAE
                     TGGDPQALASALEKLEAYAKRIPLPVNPAVSHLFIVKPLTGFSVQGLFSTHPPTAERV
                     ARLRMIARQRVNQA"
     gene            32240..33097
                     /locus_tag="VGB76_07620"
     CDS             32240..33097
                     /locus_tag="VGB76_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098806.1"
                     /translation="MEPDPKGMNDYPHSAQAGTGSLPTDLNVPSFHNTGDVVGAVFQL
                     YRDNFLSLLKIVVVLAVPLIVAQHIFLKLSRVPLAEVTIYVSAIVGESLMSGALIYAV
                     VTFLRTGAFPTLADSYTWGRKRWGRVLFCSFLYMLVVVAGCMALIVPGIIFSLMFALV
                     VPVAVLEDKPASDSFSRSRDLTRNYRLQIFLTYFVFSLLIFVISLVTNAGFGGEPEDE
                     ISLPFALVQGLITQLLESSSTVLTLFIYLGILRDTSQLSTQFPNAPPPPPPGDDPARA
                     SQVETGREF"
     gene            33291..34148
                     /locus_tag="VGB76_07625"
     CDS             33291..34148
                     /locus_tag="VGB76_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY0098807.1"
                     /translation="MVYTTQITRANPTYVIFLLDQSGSMADPFSGDQILSKADFVADV
                     VNHTLHDFVIRCTKTEEVRNYYYVSIIGYGRTTGSAFAGTLTDRTIVPISDVADNPQR
                     IETRHKRVPDGSGGVVEMPVRYPIWLYPRAGGETPMCGAFALAKSLIERWLGEHPRGF
                     PPTVLHLTDGESSDGDPTELGRQIMSLGTDDGQVLLFNCHVSTHRRRKVEYPTDDASL
                     PDAFARTLFGVSSLLPANFLGAAQQLGVNATEGSRGFVFNGDPSSVVQFYEIGTSLTG
                     MTPHIWMES"
     gene            34189..34962
                     /locus_tag="VGB76_07630"
     CDS             34189..34962
                     /locus_tag="VGB76_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098808.1"
                     /translation="MTSQHWRIETQHFLLEKEDSTPAECEDAISINAESRRYALADGA
                     TEGFDSRSWARLLVDCWTQADPAPLSVETFHPWVEEQGRRLHAVWAERALPWYAEEKA
                     RRGSYAAFVGLQFGGGDDALHWQAIALGDSCLIQRRGGAVCRALPISDASLFNSSPVL
                     VPSLDSLQAPALSRATVERGTAERGDVFLLLSDAGAAWYLKLSKERKAVEAEFDASLA
                     ASDGGALSELFRRERQAKRMINDDIAILRIAIEPARPLS"
     gene            34986..36602
                     /locus_tag="VGB76_07635"
     CDS             34986..36602
                     /locus_tag="VGB76_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEY0098809.1"
                     /translation="MSSLTTPNWPSARQFAEAVQCPAICFAEPHIKKLLPAVDRLGMP
                     LVTSGQFAYVFKLKGADGSTSVAVRCFRGYLGDREKRYKAIDAHLNLYQIPALPRFKY
                     FPYGILVAGRRFPALIMQWIEGATLDVYLEEVIDRPDVLRHLADEWLKLMSRLREAGI
                     AHGDLQHGNIIVERGQLRLIDLDGMFVPAMSGLQASEIGHQHFQHPNRDARYFDATID
                     NFSALVIYLSLISLAERPELWREYHDENLIFTKADFLDPASSTLLSRIKSIGTEHRQL
                     AEILQTAAVSSPVETPNLVDIVSPKSKLPSWMAAPLDLEVQERTREATDFELAPVVAQ
                     RLWPPPETQSAAVAPAVSASPGILNPATPVASLPSHNPLDIRGNTLMYARLLDEHTYI
                     FLWWLLLHRWIAGFWELFGIGYFSAMLLTLLLLIGTFVAYGFVRAVLDWNLASDDPLT
                     SFGSPTVLSILQSSASHAPAALPPSTRPARHLTLNADVRAVVGNSSLRIYHLPDCIWV
                     KKISPHHSLQFASPAHADAEGYRACKVCSP"
     gene            36647..36805
                     /locus_tag="VGB76_07640"
     CDS             36647..36805
                     /locus_tag="VGB76_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098810.1"
                     /translation="MRSAFEPERLVPAVREAARQNDKAQPVAEFREMEQIVSEVAASG
                     RAQIRIVR"
     gene            complement(36841..37890)
                     /locus_tag="VGB76_07645"
     CDS             complement(36841..37890)
                     /locus_tag="VGB76_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098811.1"
                     /translation="MNYHRFIVRHFPHVLLLVVCLLSLSQSLLAQTRTDQRRSPSPLK
                     ILNSNRERDGLRGLVRRLRTEVARVNLKKNGVVEGPRSLLELTVYDISGRRIDNRTYP
                     VINSPIGQETYDYDEAGRLLRTTVRDEQGVILSRTEYSYEFDAAGNWVKMTSSVVVSE
                     AGQQRLEPVEVAYRTITYYSGGVDGALPVAEEESAASQTPTGRDALPRSERVASSSKG
                     ARSASAPANVSAARPSEKEASQTRLEEAAGVAIEVDQLTDVGLVNDKALSLPGAAYPI
                     NWRKATTPINVTVEVVIDETGRVIYSRAIEGPRELWRVAEDAARRAGFLPFRTARRPH
                     KVKGRLTYSFPFDPR"
     gene            complement(37930..38370)
                     /locus_tag="VGB76_07650"
     CDS             complement(37930..38370)
                     /locus_tag="VGB76_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098812.1"
                     /translation="MILLAAFGVARAQAQNPPVSGARKIPFNRTLPTACREKRIRLSG
                     EFDVQYKVVREAGGQTLIEGNINADRINAFGLTSRAKYQAVGVGRLESSGPSPTSFTY
                     VFNFALNKTGSTDGLMGHVKFRISLNAQGGVNTSIVAANVDCTS"
     gene            complement(38567..39595)
                     /locus_tag="VGB76_07655"
     CDS             complement(38567..39595)
                     /locus_tag="VGB76_07655"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY0098813.1"
                     /translation="MTGTSLSNSPVHPERRKRFVVLLLLIILGFAGLLLVTWFAITQP
                     LISRGTAGGNVPPVDAARLEAHVRVLSGTLSPRDAGHPENLDRVAAYLRKEFEQAKAI
                     EVSEQPFESRGRTYRNVIASFGPRDKELIVVGAHYDTAGALPGADDNASGVAGLLELA
                     YLLGRIPPAVRVELVAFTLEEPPYFRSPLMGSAIHASSLRAQGFNVRAMLSLEMIGYF
                     SDQPESQHYPVSILKAFYPARGNFISVVGKMDQVRTMRRVKKAMLEASPLPVYSINAP
                     RTIPGVDFSDHLNYWNEGYDALMITDTAFYRNPNYHTFTDTPDTLDYKRMAMVVQGVY
                     AAVLALSR"
     gene            complement(39668..41014)
                     /locus_tag="VGB76_07660"
     CDS             complement(39668..41014)
                     /locus_tag="VGB76_07660"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEY0098814.1"
                     /translation="MSGIKEEQGRGKAQGAAASVDKVASISALVRKSAIPLTGGKGDY
                     DALMNLVGDARFVLLGEATHGTQEFYRERARITRRLIEEKGFNAVVLEADWSDAYRVS
                     EYVRGRGKDANVEQALSGFTRFPQWMWRNEEFRDLVTWLRSHNASRPSDAPRAGVYGM
                     DLYNLSDSSQVVVEYLTRVDPAAATRARQRTECFRRYGDRPERYGQEVATAMKTSCEK
                     DALALFQEMEQRLAAWRARPKPERDDDLFSAYQNARVVKNGEAYYRLSYLGGFNTWNL
                     RDSHMAETLQEISRYEEAIGGEKAKLVVWAHNTHQGDARMTEMGEAGELNVGHLMRKF
                     HDGASVLVGFTTYTGEVRAALEWGRQGQKMKVRPALAESYSGLFHQTGVPNFLLILRG
                     GGAVSDALSEPHLERAIGVVYLPQTERQSHYFKARLAKQFDAVIHLDVTSAVEPLK"
     gene            complement(41708..>42835)
                     /locus_tag="VGB76_07665"
     CDS             complement(41708..>42835)
                     /locus_tag="VGB76_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0098815.1"
                     /translation="ADFARGQLQKRYQLPSRGAVEIKPYRYDLIDFRTNSIRLPGIEA
                     SRGCPFSCNFCVLTGHEKYRYRPVEHVIDEIKFKMRWNKNYFGLFDNSFSFLDNNLGG
                     DPRYLRTLCEALLPLKKTWGCALTFNVLKDEELVKLMAQAGCRYIYTGLESLNPDSIK
                     AMNKGQNKITEVDRVIRRCFANGIMLSFGLLVGSDGDTNEYLEKLPEYLSDLQYFNIT
                     FLGIVCPYPETPFYRQLQSEGRILPGTVSRDYDGYTLCHRPLGLEPTEAVEHFKRLCR
                     QLGSLTNVGRHYWTKLWASNLPQYKKVILASGSEILSISNPLQNNERRYIAGLDPIEA
                     ADRRKMEELSIPPQYLSPTPALQLKTIDSRFDRGAVDLSAA"
BASE COUNT         8677 a        12682 c        12281 g         9195 t
ORIGIN      
        1 cgcatgtccg ctccagacgt tcgatcaact ccacggagtc aaccgtgtga atcaggtcga
       61 agagtttgac ggcgcggcgt gccttgttcg attgcagatg ccctatgaga tgccagcgcg
      121 ggcggctcgc gctgcggtcg agatcggcgc ggctcacgat ctcgatcttc tctcccgcct
      181 cctgcacgcg gttttcgccg aaatcggaga cgcccgcgcg catcgcttct atcacacgct
      241 ccgccggatg cgttttgctg accgcgatca aagtgagcgc gtcgccggat gtgcgtcccg
      301 cacgccgcgc gcaggccgca atgcgcgcgc gtatttcatc cagacgcgaa cccagcgcgt
      361 cgtctgctgt ccgcaaagtt tccgtcatga cgggttcaag tttctgcaca atgtctacaa
      421 agggtctacg atggctcaac tataacaagc ggcggcggac acagcaacac cgctctccga
      481 cgccttgcgg gcaggatgaa acacgcctcc gaaacgctcc ccgggtcgcg ccttgacacc
      541 ttcgctccgc gctccgtata ttcgtttcac gtctcacgcg gatgtggcgg aattggcaga
      601 cgcgcaggtc tcagaagcct gtgatggtga catcgtggag gttcgatccc tctcatccgc
      661 ataacaacag aggccggaag ttagagtcta aaggccagcc agaagcaaac tgttctgact
      721 ggcctttaga ctctcacctc cggcctcttt tttaattgat cgattcggaa gcggactccg
      781 gcgtctgcgc cgagcgcgcg tccatgatga ggagcggctg ttcgctgaag agcgtggagt
      841 tgatgagcgt ctgcacccag tattcgccgg gcgtgtgaaa ggtgatgttg acgaagaagc
      901 tgacgttatc cgtatgtccg ccctgtgcga tcacgaaagg cgcgggttcg gagacgacga
      961 tgggctgctt tttgtccggc gacaggatgc gcacctccga gagataacgc ccctcgccac
     1021 gccagtggtt gacgacggca aatttaatca gggcgaccgg caactgctgc accagcatgt
     1081 tctgaaagac gccgatcagg gataacttgt tgccgacttc caaacgtacg tcgtcgcaaa
     1141 agatcgaata ggcgagttcg agtttgttcg cttcgttcgt tgtgctcatg ccatctccaa
     1201 tttcaataga ctttagaaaa ccgctccaca gccccgccaa cgactaatca ttcgtcgcca
     1261 acaacgctct gaacccttgt tcgtcaatca ctttaacgcc gagcgtctgc gctttgtcca
     1321 acttcgaccc ggcctcttcg cccgccacca cgtagttcgt ctttttactg accgaagagg
     1381 taacgcgccc gccgcgcgcc tctatgagcg cagcggcttc gtcgcgcgtg agcgtctcaa
     1441 gtttgccggt gaggacaaag agcatacccg cgaaagattc atcgcgcacg ccgccagccg
     1501 ccgtgcccgc ctccatctcc gtgcgcacgc ccttgtcgcg caggcgttga cagagcgcct
     1561 ggttcccttc gtccgcgaac caatcgtgga cgctctcggc caccgtcagg ccgatctccg
     1621 gcacggcgtc gagttcttcc acgctcgccg cgccgagtgc ttcgagcgtg cgaaactggc
     1681 gcgcgaggat ggcggcggtg cgttcgccca cgtggcgtat gccgaggccg aagacgagtt
     1741 gcggcaggtc gcgcgtcttg ctcgcttcaa tctgcgccag caggttcgac gcggacttct
     1801 ccgccatgcg ttcgagttcc gcaacctgtt cctgtgtcag atcgtataaa cccgccacgt
     1861 ctcgcacgag tctcttctgc acgaactgat ccgcgagcgc atcgcccaga ccttcgatgc
     1921 gcatggcgcg gcgcgaagca tagtggagca ggcgcgcttt gagttgcgcg gggcaatccg
     1981 ccgccacgca gcgcgccacc acttcccctt cgggacgcga cacgacgccg ccgcacaccg
     2041 ggcacgcctt cggcatcttg aaacgcttct ccgtccccgt gcggcgcgat tccaccacct
     2101 tgatcacttt cgggatcacg tcgccgctct tttcgatcaa gacccaatcg ccgatccgca
     2161 cgccgagccg cttgatctcg tcttcgttgt gcagcgtggc gcgggcgacg gtgctgcccg
     2221 cgacgagcac cggctcaaga tgcgcgacgg gcgtgagcgc gcccgtgcgc ccgacctgta
     2281 cgccgatgtc gagcacctgc gtcgtcgcct gccgcgccgg atacttatag gcgatagccc
     2341 agcgcggcga tttcgcggta gccccgaatt catcctgcaa cgcggcggag ttgactttga
     2401 caaccacgcc gtcaatctca tattccaact cgtcgcgccg cgcttccatc tcgttgcaaa
     2461 actcgatcac ttcttcaatc gaagggcaca gacggcgatg gctaacgcta aagcccgcgc
     2521 gctcaagcca ctccagacct tcccagtgcg tggcgaaggc tttgcggtcg cccgtgatgc
     2581 aatcgtaggc gaagaggtcg agacggcgcg cggagacgat cttggggtcg agttggcgaa
     2641 tcgttccggc ggcggcgttg cgcgggttgg cgaagcgcgg ttcttcagcc tgttcgcgtt
     2701 cggcgttgat gcgctcgaaa gttctgcgcg agagataggc ttcgccgcgc acttcaatct
     2761 ccggcaggtc gagcgcgcct ccagctttaa aacggagcgg aatgctgcga atcgtgcgta
     2821 cgttttgcgt gacatcttcg ccgcgccgcc cgtcgccgcg cgtcacgccg cgcacgagca
     2881 cgccccgctc gtaatgcaga gagatcgaga gtccgtcgat cttcaactcg gcgacgtatt
     2941 cgaggcgtcg cccgtcggcg agacgcttga tgcgttcgtc gaaggcgcgc aggtcttcga
     3001 tgttgtagct gttgtcgagc gagagcatcg ggcggcgatg cacgtactcg gcaaactctt
     3061 ccacggggcg accgccgacg cgctgcgtgg ggctgtcggg cgtcgccagt tccgggtgct
     3121 ccgcttccaa cgcttgcagg cgcgacatga gcgcgtcgta atccgcgtcg gagatttccg
     3181 ggttgtcgta gacgtaataa agctcttcgt gatggcgcaa ctcatcacgc agttgtgcga
     3241 tctcctgtgt cagatttgcc gtgcgggtgg acgatttcgc catgatgcgg tctggagcct
     3301 taagacttcg cttgtttgag ttggacgaac gattcctgcg ttgaggtttc gcgttggtct
     3361 tctctggcga cctgcgcgag acggtgtatg agccgcccga aatatttgat gccctgcacg
     3421 aaatcttcga gccggatgtt ctcgtcgggc gcgtgcgggt tcgacccggc gtagctgacg
     3481 ccgaaggaga cgatgggcgt cggtggcagg ttgacgccgc agaccgcgcc cacgggtcca
     3541 ctcgccggat cgagcgggta gacgatgggc tgcgccccgt aaatttccgt cgcggattcg
     3601 atggccgcac gcgcgacgct cgacttgatc gaagcctttg cgaaaggcgc gctggcaatt
     3661 tcgatgatct ctatatcctg aaagccgcgc gtgtcgagat gctcgcgcag gagtccgacg
     3721 acgagcgcgg gcgacaagtc cggcacgagc cggaagtcga gacgcgccac ggccatgccc
     3781 ggcaggacgg tctttgcgcc cgcctccgtg tggcctgtgt gaatgccgca gatcgtacag
     3841 gtagggccga agatcaattc tttggcgagc gcgcgtccgg tcaggccgcg cacccaatgg
     3901 ttgatcttga actcgctttt gagtcccgcc tcgtcgagtt cgagttgccg cagagacttc
     3961 atatcttcct cggcgatggg cgcgacgtgc gaagagaatc cgtcaatcgt gatgacgcct
     4021 ttaggcgaga cgatggtgga gagagcctgc acgagattcc aggccggatt gggcacgatg
     4081 ccgccccact tggaatgtag atcggtgcgt gcgccgtaag cgcgcagttc gagaaacgtg
     4141 atgcctttga agccgagact gaggaggaga cgctctttcg tgtccttgta gccgtcgtcc
     4201 cagatgcaac cgtcggcgcg cagcatctcc gggtgttccg ccgtgaagcg atagaggctc
     4261 gggctgccca aaccgtcttc gccctccacg atgaagcgca ggcagagcgg cagttttccg
     4321 aaagtttttt gataagcctc gatggccgcg agcctcgcca cgaggtcgcc tttgctgttc
     4381 gccgcgccgc gcgcgtagag tttcccgtcg cgcaccgccg ccgcgaacgg acccgaagac
     4441 cattcgttca actgcccgac cggctgcacg tcgtaatggc tgtaagcgat gtaacaatgc
     4501 gaccccgcgc cgaactcgcc gtagatgatc ggataaccgc tgccgagttt gaaggaacgc
     4561 gtgccgccgc cgatgcgctg catggattgt tcgaccgtct cggccatcgc gcgcatgccc
     4621 gtgccgcgcg ccgctaccga aggcatgcga cacaaagcct gcaatcgctc cgtaaaggcg
     4681 tttgcatgac gttcaacgta acggtcgaag cgtgaaaacg gcataagaga acagtgacga
     4741 gtgacaagtg acgagtgacg agcaagttaa aaccgttgcc gtcgtatctg actttcgtgt
     4801 gcgtgacgca tgacgagatc aaagacaagt ttgaagacag ttcttctctt ctgttttaac
     4861 ttgtcacttg tcactcgtca ctcgtcacta aaaaaaagat agcacggacg cgcgccgtcg
     4921 cgctcgaagc cggcgcagac gagtccggcg tccaggtgtg cctgaaactc ggagcgcacg
     4981 cgcaggagtt cgcgctgcgc cgtgagtccg tcttcgcgcc tgagactgtt ccaatcgggg
     5041 ggaatctcga cggtggcggc gggcggggag gcttgcgccg ctcgcacgcc ccgcgcgatc
     5101 tgttcgacgc gcggcgtgcg cagttcccac tgcgcgatca ggcggtcgct atcaatattg
     5161 aactgttttc cgtccgcgaa ggcttccgtc ccggcgtccc agccgtagaa gttgacgccg
     5221 taggagcgta tgacgacgcc gaggcggttg agattgaagt gcgcattgcg cgcctgcatc
     5281 ggctcgaaag tccagcggat gaagttgcgc ccctcggcca gagcgcgctc gcgttgcgcc
     5341 catttgagcc gcgcgcctaa gccacggttt tgatacgcgg cggagacggc catcatgtgc
     5401 gagtagccga tgatctcttc tccgcgcacg gcgacgagat gatggacgaa gccgacgagt
     5461 tcgccgctcg ccgccgcaaa cgcgccgagt atccagccgc ccgccagacg cgagacgatc
     5521 aagtggcggc gcggtgagag ttccagatcg ggcatgtgga acacttcgcg ctgcaaacgc
     5581 acgcacgtat caaactcttc aatcgtcgtg cattcgcgaa tcactacctc ttcatcgcca
     5641 cgcgcgccga caatttttcc cgtgtgccct gacatcgaaa cttttgccct tccgcaagcg
     5701 tgttgtcggg tggaactcga aggataataa atcaaacgac gattctcgtg taccaacttt
     5761 ccggcacgcg cgcaatgcgt cgctcgtcgg tgcgcgccac gatgtcgctg cggaagttga
     5821 tcaggacgag gcgttccggc tcgcggtagc gttcgtcaat ggcagcgcgc agttcttccg
     5881 cgagcgcgtt cggatggctg atgattttga agaggacgag gcgcgcgacc cctgcatgtt
     5941 tcaagagcca gtgcgtcttg cgcatggcgg tctgcccgca ctcgccccag aaagcaaaag
     6001 gagcttcaaa ctcacggcct ccgccgcgcg ccaccagatc gggcttataa cgcaggccga
     6061 cgcgctgttc gatctcaagt gcgggaaact caacggcgaa catcgcgtag ccgagcgcct
     6121 tcatcaacac gtgctcgtaa ctctcgcccg tccgctgcca caggcgcacg cgtcgccccg
     6181 ccagttcgaa gtcgtaatgc atcgccgagt ccagaagtgc aaagcctcaa gtcagaagtc
     6241 cgtggaacgc gtctgacttg agactgcggg acttggggct gcgggacttt ttcattcctt
     6301 cgtcggatac gcgggaagtt cgagacagac ggttagccct ttgggcgcat ccgtgttggg
     6361 cacgattctg ccgcccagca gggcgacgag gcgttgcgcg atgacgaggc cgagttcgtt
     6421 catgttcgtg ccgcgctcgt tcggcgcatc aatctcgatg acatcgaaga tgtgcgcggc
     6481 atcggcgggc gggtcgcctt cgtcgtgtat ctcgatgcac agttcggcgg gcgtgcgctt
     6541 gaaagcgttg atcgagacta ggccgccgga ggggctgcga ccgatggcgt gcgcgaagaa
     6601 gttgtagagc acctgccgga gtttgccttc gtcggagacg attgtgccgc agtcgggcgc
     6661 agtcgtgcag tcgagtttca cgtccttctt ctgcgcgagc cgcacgaccg ccccgcacgt
     6721 ctcgcgcagc gtttcgcgca cggagaactc gtgcaggaaa agctctgtct gtcccgcttc
     6781 gagccgcgag agatcgacga gttgtttcag actcgtcttg agttggaggc cggagttctg
     6841 aattttctcg cagtagcgtt gttgcggcgg cgtcaggtgt tcgtgttcga gcaggatctc
     6901 cgagaagccg agcatggagg tgagcggcgt gcgcagttcg tgcgacatgc gcgcgagaaa
     6961 gagcgagcga taacgcgcca cgcgctgcaa ctcaagattg gccgcttcga gcgcgctgct
     7021 ccgctcgcgc aactccgcca cgagttgttc gatctgctcg cgctgttccc tcatcgccgc
     7081 cacgcgtcgc gcattgccga gcgcgatgac gatgggcgcg gcgatgaagc gcaggccgtt
     7141 ggcagccgcg cgcaggcgtt ccgggtgcga tgagatggcg acgatagaac cgatgagcgt
     7201 gccgcgcgca tagatgggca gggcgagagc cgcgcggatg cccgtctcgg ccagaagggc
     7261 gcgcaccgca gactccgccg cgccctcgtc attcgcctct ttgtccagcc acacctgcca
     7321 ttcacgcccc gactcttcca cgccgttcga cagcaggtgg ccgacgcgcc gcccctgagc
     7381 ttcgtccatg tgcgggtgtg tgtgaatcgc gctttcgcgc aggcgtccgt ccgcatcgcg
     7441 caggaaggtg atcagacagc agagattcca gtggcgcagc aaaagttcgg agaaggcagc
     7501 gcagaccgct gcctcggtgg catactcatc cggcgcaaaa atctgcgtgt agaagatgtc
     7561 tacaacctct gcgccgagcg cactgagttc ctctgacgca gcctcggatt catcctgcaa
     7621 ggagaagggc tgcaaactct ttttcacttg tgcgttcatg cggcaatgac cctagcctcc
     7681 cgcctgcgcg aaacggctaa acgtgcttca attgtcggga gcagctattg tccgaactat
     7741 aaatcgtcgc tcgcgcgagt gtcaacgaag caaaggatga acgcggaacg cggaaggatg
     7801 aactaaaaac cgctcaagtt atcgagtggg tttcttattc atcgttcaac gttccgcgtt
     7861 catcctttcc tctgttatga ttgcctgcgg agggaagttc cgcctttacc tgctcgatga
     7921 acggtcattt gcgcgagcgc ccactcgccg aactcatcag ggaaatcatc gccgccaaac
     7981 tttcgggtgc gcttcgtctt gcgcgcgaac gcgtgcaggc cgtcgtctat gcggaagcgg
     8041 ggcagatcgt ctttgcgcgc tcgaatttgc gcgtgcaccg gctggccgtc tgtctcaaca
     8101 ggtggggcgt gctggcggaa gataagttgt cggcgcaggt gacggagttt atgtcggacg
     8161 cggaagcgag cgcgtcactc gtcgcctcgg gcgcgctgac cgcagacgcg ctcgccaaac
     8221 ttctcgcgcg gcaagcgacc gacgtgttgc gcccgccgct cttatggacg gaaggtgaat
     8281 ggagcttcga cccacgcgca cggctcgtcg aagacgtgcg ctgcaaactc gaactgcacc
     8341 aactgctcgt cgaagccgca cgccaactcc ccgcagagtt tgtcgccgcg cggctcgccg
     8401 atgacgcaga gatcatttca cccgtcacag aactccccgc gcaactccaa ctcaacgcgc
     8461 ccgaaggctt cgtcctgtcg cgtgtggacg cgccgctcgc gctcggcgaa ctgctcgcca
     8521 tcagcggtct gcctgagaca caggcgcgcc agtgtgtcta tgcgctcgcg ctcggcggct
     8581 ttctcgcgcg ctccgggtgg cctgtcgcgc tctccccggc gcaggctccg gcgcaagccg
     8641 ctgtggcgga caaagccgtt gccgccggag tcgccaagcc tgacgtggtt gaagataagc
     8701 acgcgcccgc atccggcgcg cagaacgcgg aggcgacgcc caacccgcgc gcggagatcg
     8761 acgcgctctt cgcgctcgtc tatgacgcag atcattaccg gattctcgat gtcgggcgtt
     8821 cggctgacgg ggcggccatc aagcgttctt actacgcgct cgccagacgc tttcaccccg
     8881 accgcttcca gcgcgacgca gacgtttcgc tgcggacgca gatcgaagcc gccttcatga
     8941 agatcacgca agcctacgag acgctgcgcg acccgcgcgc ccgcgccgcc tacgaactca
     9001 aactcggcac tcagatagac tcttccggcg cgtcttccaa accctccgcc gggtcgagcg
     9061 ccaacacaca cctgacgcgc gagcagcgcg ccgcagagag tttcacgcag ggagtcgccg
     9121 cgctcaaact gaacaacctt tcatccgcca tcacgttgct cggcgaggcg acacggctcg
     9181 ccccacagca atcgcgttat cacgctttct acggcagcgc gctggcgcgc gacgtacgca
     9241 cgcgacatca ggccgaagcc gagttgcaaa ccgcgatcaa actcgacggc aacgatcctg
     9301 cgtatcacgt catgctcgcc gaactcctgc gcgtgctcgg ccaatcgctc cgcgccgaac
     9361 gcgaactcga acgcgcgctc tccctcgacc ccaatcacga cgccgcgcgc cgcttgctcg
     9421 accaactgcg aaaaaggaaa taacagtgac gagtgacaag tgacgagcga caggtggcga
     9481 gtgacgggtt gggaaccggg caaacttcta ttaagcgtga gtgacaaaac caccttccct
     9541 cttcctttgc gccttcgcgc ctttgcgtga gttaatttcc ccggtaaaga taagtctcag
     9601 gcaaaggcgc aaagaaaact cagagcgacg ggcggctgac ggtgttcgtg aaatccgctt
     9661 aagttaacct gcctgattcg acggcttttc tattcatcgt ttacgattta ctattcactt
     9721 ctttctgact tgtcacttgt cactttttac gactatggct tctctgcaaa cgaaattaac
     9781 gaacgaagac gggatcgcgc gcgaggcgtt cacgcgtctg gcgcgggcgg cagatgaatt
     9841 tgagggctac gctcacccgc acgcggaacg gatcgctgcg ttgtgcggcg agttggcgaa
     9901 actgttcggg ctgggtcgtg cggatcgcgc gtccctgcac ctggccgcgc tcgcgcacga
     9961 cctgggcgag ttggcgatga agcgcgatta catccgccgc gccgcgccgc tcaccttcga
    10021 ggaacggctc gatctggcgc gtcatcccgt catcggcgaa caggaggccg cgcgtgcggg
    10081 cgcagaccgt ggcgcacaac tgctcgtccg ctggcatcag gaatggtgga acggcgcggg
    10141 ctaccccgat gcgctctcgc gcgaacagat tccgctcgcc gcgcgccttc ttcgcgtcgc
    10201 agacgcttac gccgcgctca cggatgcgcg cccgtttcgt cccgcgttca cggaagagga
    10261 ggcgcgccgt cacctatccg agtgggctgg actggagttc gacccgaacg tcgtccgcgc
    10321 ttttctcacc ttgcagaatc tgcccgcgct ccgctccttc gcgcgtcgtg atgaagatgc
    10381 ggcggcgacc gcgacgcacg acgcagcgac gcaagcagac gagccgcgcg ctgacgctcc
    10441 ggcagttgac gccccggcac ttaacgctca gggatggaac atcggcggtc atattcccga
    10501 agcgggaacg aacccttacg tccagcgcgc cgagccgaac gcagaaagcc gaacgcccgc
    10561 ccctgagcaa agattcgaca ccgatcaaaa aacggatagc cttcgagact atgacagttt
    10621 aaggaaggcc gattaaagac cgtctggaaa ccgtctggaa accgacatgc ctgattattc
    10681 caactcaacg ccgcgcggct ggctcgcctt cgagttgagc gtcctgcgcc gcttacgctt
    10741 tcgttcgctc attaatcctt tcgcgggcga gcctgatctg gaaacgcacg tcaagcgttg
    10801 gggcgtgcgc gtcgccgcga acgatgcggc gcgctggagt tggatcaaag ggatcgcgcg
    10861 cgtcgaaaat aacggcgagc gtttgaccgg agacgatctg gaagtgctgc tcgaagacgt
    10921 ttacgtgccg cgctaccgtc tgcgcaatgc cgcgctgcgt cgctggttct ccgagacgga
    10981 cgcgtggtgg ttcgacaatc tgcgcgaaaa cgcggagcgg ctcgaaggcg agatcaaaca
    11041 atcgctcgca ctcaatctag ggatgctcgt cggcgactac gccctctcct tcgacgaaga
    11101 gacgcgcgaa ctgcgccagc cgctctcgcg cgtcctgcgc cgcctgcacg agacgcagcc
    11161 gccccccttt gacaacggac agccgaacac gagcaccaac aaagaggcgc gccagttcgt
    11221 cgccgaacag agaggcgacc tgctctttct gcgcctgccc gcccctgcgc gcagcagtag
    11281 tggcggcgcg caggggcgcg aggggcttcc ggcgtggcgc gaggaatggg tgcgcggcgg
    11341 ctctgaattt tgggatgaat ttgaagcgtc gcgtgcgggg cgactcggcg ggcgcgtcga
    11401 aaccaagggt caacacctgc gtctcatcga agaactgctc gaagccgccg cgcacctcaa
    11461 gtcgtgggct atcgcgcaca cggaaggcgg ctacaccacc accgaagaac tcgtcgagac
    11521 gatccgccgc gtacgcaaag tggaaaccat ctacaccaaa gacttctccg aactactcgg
    11581 cgcgcgcgcc acgatcatca cgacctgaaa gaggcgaatc ctgaacagat aaaacccgga
    11641 ttccgcccga aagccctcaa tcctgcccga aatgtcgccc gcgttcaaac ctcgcgggca
    11701 caagcccgtt tgattcgaat agaaataatc agacatccct aatctgacca gcctacatct
    11761 caccaataat cgacagctat cctgatctcc tcacttagtc tcctgaggag tgtccaacag
    11821 gtataacagg agacaggaag taacgactgc cctttcggag gaatgaaatg aagaaagcct
    11881 tctcactaag tctttgcctc atcgtatgcg ccttcactgc cgacgctcaa acgcgtactt
    11941 ctcagacacg tacctctcag gcacgcagac cgagcacggg cggcacgcct taccagcgac
    12001 cgcagaccga caagcgcccc cagaccgaca ggcgaccgca gcccactcac ggtggcacgc
    12061 cgtacgagcg gagcgcgccg ggcggaactc cctacggcgc gccgtggtta ggtaaaagac
    12121 ccagcacagg cggcactccg cccagaagac cgcagaacac ggcgggcggc acgcctgcgc
    12181 gcacgcctaa taccgccggc ggaactccat atcgccccac gccgaggccg agcgcgaaat
    12241 cttcgcgcgg gcgatactga gtttccgtct gtgtcctaac ggcgaagcta cgcaaaattc
    12301 ccgctttccg ggcaaagtct aaaacctgtt ttttctattc acgcttcacg gcttccagag
    12361 cgaatttcaa ttgagtgtaa gtagaagcag tcaccacaga gacacagagc acacaaaggg
    12421 ggcacagaga tcattcaagt ctctgtgccc cctccgtgtg ctctgtgtct ctgtggtgaa
    12481 cttatgtccc ctacattcaa ctgaaacctg ctctagtcag tgttgcactt caaacttgat
    12541 gtcaggtttc gttccttacg gtcattcatt gcgtggacgc tcacccttat cttctaaaac
    12601 actccatccc aaggatgagg caaaaggtcg cggagctgca ctgctttagc gtggcctagt
    12661 ataacttctg tatgatccat gttgctttca tcaatctgat gcattaactc cctgcaacgg
    12721 ccacacggcg gaagaatcgt gccatcaccc aggacagcta cgatcttcct gatgctctgt
    12781 tcgccatgcg ttaccatgga cgcaattgcg ctgtgttcgg cacaaaaccc gatccctgcc
    12841 acagcatcga tgcatacacc cacgtggaga ttgcccttct cggtaagcag ggcacacccc
    12901 acgtcgccga caaagagtcc gtgtgcaatc tttctcggat tgaccacagc ttgagccttc
    12961 tgaattaatt cgtcgttcgt catcattcta ttttgtgtgt cctgacattc gacagaaatt
    13021 aacgtccggc atcacccgtc cactgctgac atagatcatg agagccgcac tgctgcgggt
    13081 cgggggcacg ccgttgttag gccgccgggc ggaaatcagc ggcctctgac ggtgttgtgg
    13141 tcttgcatga ttagccaccg gccgcctcgc ttcaccacga cgaaagtcct gatctgccgc
    13201 tcgttctcgt gcttgattcc atcgggcgtt gtgtaggtac tgactctgct aggcacggtc
    13261 acgaccgcca ctgcaggtgt ggcgaaacga accgacatct cctcaggtgt gtcggagacg
    13321 cctttgagga atgtggagtg gacttccttc acctctttga gcaccgcttc ccgcccacgc
    13381 gtccagccgc ctgccggatt gatgtggttc cagtcttccg tcgtgtattc cgccgcacga
    13441 ccgaaatcat ccgaactgaa ggcgtcgtag aatgcttgca ctgcttgacg tacctttttt
    13501 tccttgccag atgtctgagc gaaaacacca ctgacgcaac acagacatat taaggcagac
    13561 agcagaagtc tcatatttgt cccttcgcct ttagttatag gcggactaac gaccgaatta
    13621 accggcgagc aatcaatgtt gaagatgaaa gacctgctga ttgcgagtcc ggttgaatga
    13681 gttataggtt gacgaggttc atacttcaac tccgattcgc tagagttgga gcggggaaag
    13741 aacgtgccgg gcggggtgac gaatcttgaa tgaaagacgc ttctcgacgc ctctaacaac
    13801 ctcttcgcac acccgcccgt ttgctttagc tcgacaacga actcttagat gatgcgcgcc
    13861 tcggcggctc actataacta tttcgcaggg ctaaggccag catgttctcc gcacttgttg
    13921 actctcttcc cggaggactg ccaggactca atacgctgcc cgcatcggca tcgactggtc
    13981 tgatcgtaag catgacatct gtcccatcga cttggcgaca ggagagactc aggcttccat
    14041 cctttctcac tcgccgcaga ccattgacga gtgggcgacg gctctcagag cacgctttgg
    14101 cggacgccaa ctcgccctct gcctggagca ggcgcgtggc cctctcatct atgccctgat
    14161 gaagtatgac ttcctgactc tctaccctgt caacccctcg accctcgctc gttaccgcca
    14221 agccttctcg ccatcgcgcc ataaggacga cgcgccggac gccgcttacc tcgccgagtt
    14281 actcctccac cactgtgaac acctgcgggc gtggcggcct gatgatgaac agacgcgcac
    14341 gcttcgctat ctggtcgagc atcgccgccg tctggtttct gaccgcactc gcctcagcaa
    14401 ccggatgacc tcgcgcttga agtgctattt tccgcaagtt ctggactggt tccctgacat
    14461 ccgcactgac ttggtctgca acttcttgct ccgctggcct gcactggagg cattgcggcg
    14521 ggtcagacgt gaaaccctcg tcaagttctt ccaggagcat cactctctca ggagggagac
    14581 tatccagaag cgagtggaag ccatcaagca gagtccgcca ctcacgacag accgcgccgt
    14641 gctcagttct tcagtcgtca tgagcaaggc gcttgctgcg cagatgaagg ccacgctcgc
    14701 tgcgagcaaa gagttcgaca cggagatcgc agtccatctg ttcgatgcat caagacttta
    14761 tgctcttcaa gcgcctgccc ggggtgggtc acgtttatgc ttcccgccta gtcgtcgcct
    14821 tcggcagcga ccgggagcga tggcaatcag ccgatgaagt ggcgcaactc tccggcatcg
    14881 cgcccgtcat tgagagaagc gggaagaggt gccgggtgcg gtggcgctac ttctgtccga
    14941 agttcctgcg ccagagcttt cacgaatatg cgggagagtc ggtcaggcat tcgttctggg
    15001 ccagagctta ttacgagcag caacgcgcaa agggtaagag ccatcaagcg gcggtgcggg
    15061 cgctctcatt caagtggatc aggatcatct tcagatgctg gcagacaaga actgcttatg
    15121 atgaggtgaa gtacttggag agcttgcgca gaaaagggtc aacgctactc atttatgcgg
    15181 cgaataatcc ggcctgatta agagtttttc cttgactcgt catcctcaga tgggttgtca
    15241 ggccgcgcgt caagcacacg ctggacaacc tcacttacgc actcgtcaga tgaaaatccc
    15301 gccgcgttca tgatgcgcga ctaacccctt ttacctttat gacgtttcct cttggatttg
    15361 cctccacctg cgtcgccctt cgctgaattt gcgcctgcaa cttcttcccc gcccttagag
    15421 ttatacctca gacagtcgaa tatcttcagc gagtcaatga ccttcaccga gtcgatatca
    15481 tcgtggaact tcaactcgcg gaggtccgca acctcgtcat taggaccaaa agtcaccaat
    15541 tcatctctga agttctggtc gcgataagcc aggacgaagg cattcggccc cacccgcaga
    15601 cttcctatgt catcacccca atcgccttga acgaaacgca ggtttggatg ctcagccggg
    15661 ccgtagatgc gaatcacttc gcccttgaag tttacgtccc gccacacttc tataaaacaa
    15721 tcgctggatg tttcttgcat aaactttatc ccgacggcat ctcctcaaat cacgtgcggg
    15781 tacactgcat tatctgacaa acttatgttt aaaaactgcg gtcgcccggt ggcctctagc
    15841 ggcgcaacga cacgtactcg acgctcttcg actcgccgaa gagcacgcgc aggctcctca
    15901 ccccgtgtgt ctcctggggt agttccccgg ccacagcgac accaatccga ccgatagctt
    15961 cgccgatgga ctttcgctcc gccggggtcg ccttcgagta gcccggcccg aggttgatgg
    16021 ccacggtcat ctgcccgtcc cgagcggcct ttgtgatgcg accggcgagc gaggacgcga
    16081 tggtggccgg gtctgcgaag tcgtccgggt cgcttccgac ggggttcggc atgagatcgt
    16141 actcggtcgc cgcggctgcg cctgccgcca ccgcgcgagc cattacttcc cggtcaacgc
    16201 gggcggtccc ggcttctggc cccgcatggg gaacggggcg gggcttctcc ttcgggatcg
    16261 ggtgagcaac gaggttcacg ctcgcgagga tgtcgtcttc gcccttggac ggtgtcggcg
    16321 ggacattgtc gtcgtaggga ccgggttcgt tccttatgtc cgaaaggtta gggcctatgc
    16381 cgacgatcac tccggggatg ccggtaccct tgcggatgaa ctcaggccag acctcgcgcg
    16441 tgtgatgctt cagcgatctg gcggggtcga acttgccttt gttgtcctgt ggcttgttga
    16501 ccgcgccgac ctggtcgatg tgcgtgtcaa aatggttgcc gggaccgacc gatatgtgaa
    16561 ggctgccgtc ctccgtgttg aactcacgga gcgagtcctg ccctctgtga agtagcgccg
    16621 cccaccacgg atcaagcccg aacttgccgg actcgaagag ccctatcaca agcgccgtcg
    16681 cgttgtggga ctcgaatccg atgccgccag cgctgcctgc gactatggcg cgaccgccat
    16741 acgccttccg ctccggcttg tcgtgctcgc cacgaatctt cgcgacatgc ttccacaggc
    16801 cgagcgccga cagccggttg taggcctccg acaggctgat cttctggcgt gtttcgagtc
    16861 ctgcccagaa cacgtcaggc cagtcgaacg acttcgcgag cacctccttc agaggccccg
    16921 gcagcgagtc gaagtcctgc cgcgccgcgg cgttcgcctt ctcgcctccc cccgtatccc
    16981 cttcgttgtc gtcgcgttct ctccccgcga cgcgctccat cacttcctcg tcgaacagat
    17041 cgacttgccc ctcgggcttg ggttgtcgtt ggacggcgct cgatgaggaa ccggccccgg
    17101 tcggagggaa accgtcgagc aactctcgtg cggactcgcc gcgaacgact cgatcggcga
    17161 tggcgtcggc gttccgctcg tacgcgtcgc cggaccgacc gacgcccctc tcaagccgaa
    17221 ctccgtgccg ctgctgcacg acgtgcgcgg cctcgtgtgc tgcggtgcgg agactgggct
    17281 cggtgccaaa ggccacactg tccccgacgg tgtatgcctc cgcctccaag ttccgtgcag
    17341 ccgctgccgc gcggctcccg acgtaggccc tcacttgagt gagatgatgc ccggggccga
    17401 aagattgctg gatccggttc agatagggca gaggggcggc gttaccggcg gtcccattcc
    17461 gggcgacccg gtgtatgtcc tccgcacgtg ctttgggata cagcggaatc cggctgaaat
    17521 catgtccggg acgaccaatc ccggtcgaat cggaaacggc gccgagtccc tcaggtttag
    17581 cttgcagtgg ccgccgcccg gcttgagtcc cgctcgtgcc ctgtaggtga aggctagagt
    17641 ttagctcacg attttgcgca aactgcgcgc gaccgggtgc cgtagattcg gcagacgtat
    17701 tctgctgagt tgccgttggt atttgtgcga acgtctgcat ggttaatttc cttatctttt
    17761 atttcgaagc ctaacgctcc acattcgccc gcatgctcaa gacatccccc ggaagccgct
    17821 cggcttcagg acgccgcacg aatgcttcaa cgagtcctaa tcagcgttgc acttcaaagt
    17881 tgatgtcagg ccgcctcggt gcgtgccacg aatcgttcat agtatcgggg cgacgcattc
    17941 agggacagtg ttgcaaaaat agccattcgg gtattatctc accctcccgc tctgtcgact
    18001 gctcttaaca agtctccaca tgaagcataa ctccacacca ttcagagggc ggcatttcga
    18061 gcccgaactc atcttacttt gtgtgaaatg gtactgtcgg taccaacttt cttatcgtga
    18121 tgttgaagag atgatgcatg agcgcggact cgatgtagat cactcgacgg tgtttcgttg
    18181 ggtacagaga tacgcttcgg agatcaacaa gcgaatcagc cctcattagg aaggtgatga
    18241 gggctgaaca tcgtcgcctg ccattctcca tcagtgtcga taagaacgct ccttacccgg
    18301 aaaccttcag cgcttcacaa gcggaacggg ttgtgccgca ggattacgca ttaagacggg
    18361 tgaaatatct gaacaacgta atcgaatggg atcatcggtt catcaagaag aaggttcggg
    18421 catcacgatt acggtgcgtg cgtgagcggt tagttcctct cgctgctctg cactcagata
    18481 cttatagcca cggatcaatc cgaagcaatt ggccgccccg caattgcagg tgaaaggcgc
    18541 ggccatttca tactccgtcg ttaaataatt aaaagtacac tcctctccct gaccgatctt
    18601 tctcaacggg cgaaaggtca cgctcgcagt attgatctca ctgttcggct gacagctatg
    18661 gttcaagtac ttccagaaaa aattctggtt ctgagttacc tcttggtcgg gcgggacact
    18721 tagatgcttg tcctcgccca actggataga atgtctagtg ggatgactgg tgatcacgcc
    18781 ttctagcttg atgaccgaat tcatcgcgat ctcttctttg gcgaagacac ctctaaaccc
    18841 gttcttttcc cgaatctcta tttttaaact ttccggcttg ccagcgtcag ttccgccaaa
    18901 tactggcacg acgatgaaag tattgcgcat aactatcatc tgtcctttcg accatcaatc
    18961 tctctactta atgcgcttgg cgatgaaaaa agtatcatgc cagccatttt tatttgctcc
    19021 cgggacatta tttgttcctc ttccattagc gcattagctg cacccgaacc ggcagggcag
    19081 tgttgcgaga attattttag agcgcagaag gtcacaagtg actctcatca agcggccatc
    19141 agaaacagcg aagggacagg cgaagtttgt cgcttcgttg tttcagattg ctgcctgagg
    19201 aaaagcagct tgtcactttc tgcgctctaa aataattctc gcaacactac cgccgggagg
    19261 agtttttatt gtggctcgtt ttgggagcgc catcctccca gccagtgttt gctcttcaag
    19321 cttaagctac ttgcgtgagc ggcctccctt cgaagtcttc gacttgccac cggtttgttg
    19381 gttgcgtgta atcacctcca acgcattgtt gtagatgccc gggctgagtg acggctgccc
    19441 cgtgcccaat aaccattcct caatgggcgg cttccgctca gcggcaacca ccggcgcggc
    19501 gagcgacccc ggtaatgaca acgctccggc ggtcattgcc agcccgacca gagaagcacc
    19561 ggttagaccg agaaactctc ggcggttagt ttttcgtgcc ttcatgtcta cctcctcatt
    19621 tgtcaacaga ctactcggct gtcggacgca cctcccgctc tacagtcttg tagaggccgg
    19681 gtgtcaggtg tgggctgccg tatttgagca gcaactgttc gatccgcctg tctttatcgg
    19741 tgagatactt gacttgtcgc ccgtcatcgt agcgaaccct gccccggtaa accgtgatcg
    19801 cgctcctctt tccggccacg gggctggtca tgttcacctg aaagccccgg tagcccaagc
    19861 cgtcaaagaa gtcgcacggg tcggcggctg gcaacccttt gacgatgaca agcaagttcc
    19921 ccgtctgctc ttcggtgagc gaccaggtgg gattcttcct cccgctaaag atgtccagcg
    19981 tgacctttgc ctgtcggtct gccttattgt ttctaccgcc caagtgcgac ctcttttgct
    20041 gactcacggt taaggtacaa gccaaaagta acgcaagggt taagacaaat actttcatcg
    20101 tcaattgctc ctcatttgca cgcgggatca gcgtacagtg taattggtcg ggaaatacct
    20161 gtaggagcag aagatcgtgt atggcccacg atcacaggta taagggtcgg taatccagta
    20221 gccgctgctg tcgtagttgc gcgcagaggt tttgccgggt ttgtggcacc agtggccgtt
    20281 ggcgcacttg cgataccagt ggaaatcgcc tgataacggg tcggacgtat agggccagat
    20341 gaccagcgcg gcgtaatact gtgtgctcgt ccagcagttg agattaaacc cgtcgtagga
    20401 tgcggcgtag ccgatctgtc ccggcgaatt gccgcaggcc agatcgtacc attcgacgtt
    20461 cgccttgcgc cccggctgcg cgaacgtgtc gctgcggtag ttggcagcga agttataaca
    20521 gttgttgttc tgcatcacgt aagggtcgga gttccagtag gtaaagttcg tgtcgcttgt
    20581 gtaactgaaa gccccgcagg ccagcgcgct catgctgtcg cttgccgctt tatcatttat
    20641 ctgcccctct tcgctcgaag gagttccgcg accgtcggga tcgggaacga tgatcgtccc
    20701 atcaatgagc ggctcgtgac ggttggcggg cgtcagaggt ggcccgttct ccgcatactc
    20761 ggctcgaatc caacgctgtg tccaggcgta gtttgcggca tcgatgtact cttgagccac
    20821 tttgagcacc tcatcagccg ctgtctgtcc ggcattagcc gtattgagca aaaacgtctc
    20881 cgtctcccct ccccgctcgt gccccgcgct gataaagaaa ctcggcggta tgcctgccgc
    20941 gtgccaacgg ttggcgcgct ccccgcccac tttgatgccg aatccccggt atccgagatg
    21001 ccccactttt cgtggaggtg ctgacatgtc cttgtcagcc atgacgcgag aaaacagttc
    21061 attctcctct ttgttcgtga gcgtccagag cggattggga cgccccgaga agatgtccaa
    21121 ctcgatagta atcataatgg tttcctctcc tgtagaggct tgttgcagag aagaatgcct
    21181 acagtgcaat ctcctctgat aggtgatttt gtgagtcagg gtgaaaagca acttccgccc
    21241 ggggacaaga agtatggagc actaatgcag acgacaccga ttgaggtgtt gaccgatgcg
    21301 tttaacgttc ggagctaggg atagaggaat cccacgtctg aaaaaaggag cttctattca
    21361 aacagttgtg acctcagggg ttgtaaggta aaagagttaa cgagtagagg gctcggtaac
    21421 gatttaaggg gaaatagcgt tgccgactct actcccaccg gatggggatg tcaataaaaa
    21481 tttagtctga ccgcttatca cttttgaggg tagtgatgtt atgaagcagg cacctccaac
    21541 tcatgtgtga gctgcgataa actcctgttg cgcatgagat cgccagggga ggtgcctatg
    21601 acaacctatt gtggcgtgga ctgcacgctc gtcaacagac tatttgttac tgcgacacag
    21661 tcgacggcct catccagtgc cgcgaacggc atcacaaccg ggatgatgtt cgtgccttct
    21721 acgctcagtc cacaggtgac gtggtcgtca gcttggaagc cagcggctac agcacttggt
    21781 tcgtcgaact gctggagagt cttggtcatg aggtcttcat cggtgatgcg accgggattc
    21841 gccgtcgcgc acgccgcaga cacaagacag accgtcgcga tgccgagctg ctgctcgacc
    21901 tgcggctgcg acaagagttc ccgcaggtgc atctgcctgc gtttgaaagt cgggagatat
    21961 tgcggctcct gcgctaccgt caccggctgg tgcagatgcg caccaagtgt aagaacagct
    22021 tgtaggggct ggccttcagt gcaggcttcg gccaagcgag cgacgctgtt gagtcacatg
    22081 gggcgtgagc gtttcctcga actgccgatg agcgcggcga tggcgcggca acgaagcgaa
    22141 tggcttgctt tcgtggcaca gttcgatgaa cgcatccatg aactcgatgg ctggtttacc
    22201 gggcaagccc ggcaggatga acgggtgctc agattacaga ctcatccggg catcgggttg
    22261 ctcacttcgc tggcgctggt gtatgggctg gaaccgatgt cgcgctttgc cggaggcaga
    22321 catgtcgccg cttatgtcgg actcgaaccg atggaatact cgtcgggagg taaacaaaag
    22381 ttcggcttca tgagtaaagg cgggtcacga ctcttgcgct atctgctagt ggaagcggcg
    22441 caaacagcgg tgagaagaga caagcagtta aagagattct atctgcggct ctgcaatgaa
    22501 cgaggcgcgc agaaagcgaa agtcgcggcg gcgcgaaagc tgttgattcg cagctacatc
    22561 cttttgcgtg acggaataga ttacgcggag ttcctcgcaa gaggtgtcga agcacggcct
    22621 gcccggcttg caacataggc tttgatgcct gaggtttgtg attgggcgac cggcctcttc
    22681 ccgcagggga gagagcgaag taaacctcat gggccttttg tggcagtgtt gcaaagttta
    22741 ttgtagagcg cagaaggtca caagcaaccc ttcatcatgc cgctatctga aacagtgaag
    22801 cgacgcactt cgcctgtccc tgtgaatcac ctcccgttaa ccttttgacc tgacttttcc
    22861 tcatcatgtt tgcggcttca atgccttcca gtgttcgttc agcggtgtga aatcgcctga
    22921 agcattgtga tgcctgtacc ttcttcttga taaagcggtg atcctgctca attacattgt
    22981 tcaggtattt caagcgccgc agtttgcagt ccttcggtac aattcgctct acttgtgaag
    23041 tgctgaaggc ctcaggataa gccgcgttct tatccactga gatggagaat gggagcctcc
    23101 gatgctcggc tctcatcatc ttcttgaaga atcgtttcgc cgcgcaaaca tcgcgtttcg
    23161 ctgagagcat gaactcaatg gtttgtcctt gcttatcgac cgcgcgatat aggtacttac
    23221 agatcttccc gactttgatg taggtttcat ccacccgata ggatgtgccg ctcatcttca
    23281 aatgaggacg aatgccgctt gttgatttca ggggcatatc tctgaaccca gcgaaagata
    23341 gttgagtgat ccacatcaag cccacgctcc tgcatcatct cttcaacatc acgataagaa
    23401 agttggtacc ggcagtacca gcggacacat aaaagaatca gggtgggatc gaagtggcgc
    23461 cacttaaagg gcgaggagtt atgcttcatg aacagtcccg atgagatgag ttgagagtat
    23521 ctgagggaga gataatacta gaaagactat tcttgcaaca ctatccccga tagttgaata
    23581 gaaaccgctt gtcgcgttat tcataaaatt cataaaataa gcattatcgg aattagccta
    23641 cgatcacatt gacgacctat tttaactccg attaaatatg aattaataag tgtgaaacag
    23701 gctgtgtaac aaatgtttga aatgtttttt tatcactgtg tcgggacact atttcatggt
    23761 gccgttactc tttgaacagg tgccttatcg taagttttag aagtgataat acgtagagct
    23821 tttctgaagc taatgacctt caacaattct tacagaggaa acttcgtaca aacattgccc
    23881 gttagattaa ttccctttcg ctcgttcatg attgcatcgc tttacaccgc cagctttacc
    23941 ttcgggatag aagaaacctg tcccgaggca atccgggcag tttaataaat taggcgaagc
    24001 cgactcccct ttttgatcta tatttatatt cttatctata ttggtgctct gttgtagctg
    24061 gctgctcttg tgtggctgcc tgctctgttg tagttggctg ctccgtgaaa gctgccagct
    24121 tggggcatgc actatatact caactccttg ctcctttcca aatccctgta tatttccggt
    24181 cttctcaatc agccctttag cgatgagttt cgctactact ttcttgactt gtgcaagtga
    24241 catattcgcc cgctctgcaa gcctggggtt gctgactcga caacgatttt gcttccatcc
    24301 ccacgataat cgacaaagtt gcatataggc agcttgttca aacgggtcga ggtgcctgaa
    24361 tagatgatct agcagctgga aaggtgtctc catatgccct ttggcgtccg gcaaggatgc
    24421 taataaattg acgcggcttt cttgtagctg gctgctctgg agtggctggc tgctatcggg
    24481 tggctgggtt gggttggcct cacggctctg cggtagctgg ctgctctgcg gtagctggct
    24541 gctatcatag ccgccacgtg gctgctctga tagctggctg ctttcctgtg gccgggagct
    24601 ttcttgtggc tggctgctcc gtaatggctg gctctcctct ggacgggatg gttctttctc
    24661 agttttagca ggtggagcgg cgttgactaa tcgcgcgaat ctactcatta aactgccacc
    24721 tcgggcacag tatcggaaat gagttcagga tcgattgtgc gcgccccttc cattagcatc
    24781 atttcatacg caagcgcagc gaccttgagg acggaacgag gaataccttt tgtgacaaca
    24841 tacagtttct ctaccgcttc agtagtgaaa gggttgttaa tactttcgtg cttacaacgg
    24901 tgtgccagca tgccgcctgt ctcttcaagc gtaagggggt ctaagactga ataggttgag
    24961 atacggctgt aaagagcacg gttcttttcg ctctgtagtt tctcgcgcaa ctctagctga
    25021 ccggcgagca cgatctgaac gagcttatgc gtatgtgtct cgaagttgag cagcccgcgc
    25081 acaagttcaa gttgcgcgtt gtcaagcact tgagcctcgt ctacgaaaat cacgacgttc
    25141 ttaccggcct catagcgatc tatcaaccac cgttcaagtt cgtcacgttg ctgtgtgagc
    25201 gaccgtttac cgtccacacc cagatcagta gccatgtatt tgaggaaagc aaagggggaa
    25261 gggaacttgg gggtgggtac gagggtcgtt aggcagttct cattcgccgc atactctccg
    25321 aaaagatagc ggagtagggt acttttaccc aatcctacat cgccgagcag tgcgcacaat
    25381 ccctgccgct tattaaccgt gaatcgcacc ttagccagca cggccttcag gccaggcgta
    25441 atatgaaggg tattaggatt cggcgagata ctgaacggag tatagtcgtc gatagaggga
    25501 gcacttgtca taatagcgcg actttatcat tccttgagat tacaatcaat gacaactttt
    25561 gttaaataca gccttaactc tgtcaactag ggggtgaata gtataactgg tatggtaaat
    25621 tggcaaaact aatggggaag gtaactcaca tcctgaactt aattttagaa tatcgcacaa
    25681 acatccctat gggggcagca atgagcgcga cggattacgt cgccagacac tttctgctgt
    25741 tgaacggcgt aaaccaggtg gctacagggg gagcgaaata ttcgactttc tacgctatcg
    25801 ttgtcggtga gttgtcagaa gaggaaccgg cgaatttggt gagcacctct ggaaagcgcg
    25861 agcagagagg atgaatagta ctgactcggt tacagagtcc ttcattaggg atgcattcaa
    25921 ttaattaaac gtgcgggata aagctagtgg cagagtcatt caaatttgag tctcaagttt
    25981 gccgataaat tgatgcgtgc gggtggcgtc ggtcagctcg gcgtaagcat tggcaaactc
    26041 ggcgcgcgtc atggagtctt cggtaaaggc tactcaagac atagaaggcc gtggttacca
    26101 agacattcgg aaaccatcgg gcggaactca tctcctgttt tcgagacgct tgatctctcc
    26161 ctcttctgcc atgaacaaac ttccatcttc gccgtcaatc tcatacccga ttatttcacc
    26221 cgaatcataa ccctgacgaa tacgccctct gccgcgtttg gtagtaaccc gttcgccctt
    26281 cctgaatttg aatatcgtcg ttgcgtccgg gctatccaac gtctttagac tgtcgcgcca
    26341 gtttgtcccg gcacgccaat gaatgtggaa cgggccgact ccctgaactt gaacaatcgc
    26401 tgcctcgggc gtgtagccga acatcgtgct cccttttgga atgaatgcgt aacttccggc
    26461 cttcatctcc ttgagagccg cgcggtcaaa tttatctccc acgccgaaat atagtgtccc
    26521 ttgcaccacc gtaatatgct cgtccacagg atgtttgtgc ggagggatga cgccgccggc
    26581 aagctcccgg attcgcatca cgaaaggctc gcccaccttt tcagggtcgc catatagaat
    26641 ttcgacatct tgagacatct gcttgatggg gataatgcgg tgcccgccag tctctatgga
    26701 ctgtcccgac ccgatactcg aagcgttgca aattacaaag cagctcaggg ctaacgtgta
    26761 gatgactcgc ttcaacattc cggcacctcc gacagaaaga ttcttttaag actctcacgc
    26821 acgagttaac ctgcgccgag cgcaagcacc aactctaagc gagcaaagta cgataagagt
    26881 ggcatggaat tgcctgtgta agttattcgg aacaagtctc tcgcagcgag atattgaggc
    26941 ttaaagaaat ttttgcgaca ctgcctcgtg gggcgataat agctgacagg ctattttcaa
    27001 cgctactctt ttttagttgg gatcggaaag cctcgataga agaggtgaga gataggagct
    27061 tccgaggtgg aaatgtatat caggaaactt atcgcgattg caggttgtct ggccgtttgt
    27121 gcaatgagcg ctttcgcgca ggggagtaaa ccgacagtta accgcgatcc ggagaaggtc
    27181 aaatttgtta cctctgacat cgacaatttc tggcgcgcgt tcgatctggc ggcgaaagag
    27241 attgacaggg agaagagaat cgccatcttc caaaaagaat atctcgatca gggcagtgca
    27301 gggttgcagg acttcgtgcg gttaagaata aaaaacgcga agagtctggt tgaaacaatc
    27361 gagaggctgc cgaagttcta cgcctcggtt cgccacagca cgtcgcgcgt ggcgcagttg
    27421 gagaagccga tgcgacgcag tttccgcaaa ttcaagcagc tgtatcctga cgctgccttc
    27481 cctgatgtct atttcgtgat cggcatcgcg aataccggcg ggacggcgtc gggaagcggc
    27541 ttgctcatcg gcgcagaact gtacgggtta acgccgggta cgccgcgtga agagtttccg
    27601 gcgtggttca agactctact gcccgatgcc ggggacgaaa aggaactgct gcggctggtt
    27661 gcgaaggctc tcgatacggc tttaaagccg gtcgaaaaat tgcctttcac cgtcgcgcac
    27721 gagtcctgtc acttcaatca aaactacccg aagcaggcga ccctgctcga aaaatcaatt
    27781 caagaaggcg cttgcgacct catcgccgaa ctgaccgccg gacaggtgat caacccgaca
    27841 ctaaaggtct acggcgaccg caacgaagcc cgcttgtggc gagactttca ggccgagatg
    27901 agtgggacgg atttgagcaa gtggatgtat aacagtttca ggataaagga tcgaccgggc
    27961 gacctcggct actacatggg ctacaaaatc tcacaggcgt attaccggaa cgcgaaggac
    28021 aaacgacagg caatcagaga cattttgaac atcaaagact ttcaaggttt tttccagaga
    28081 agccgttacg atgaaaagtt tgcagcgcac ggtgcgaagg cgcatccttg aaaacgaagc
    28141 ctcacgccct gcaatgcacc cgacgtatgg atgcgtgcgt tgattccgat tctcttcatg
    28201 cgggtaggca ataggatgtc aagaaaaccg tgggaaggag cgccggagcc tgagcgtttc
    28261 agggtgtctt gaatggtgaa aacggggtat catttgaacc caagtttgat gacgcctgtc
    28321 gtaatactaa actgaggatg aggcaccaac taacaactcg actcaagtta tattcctgta
    28381 tagctctcct gatcgcgctc tgcggggcgg gttcagtccg ggcgcaacaa acggcggtga
    28441 cgatctccgg gcgcgtctct gaagagagca cgggacaagg tatcgacagt gtcgccgttg
    28501 tcgccctcgg caaccagacc gggacgcggg ttgccattac cgacgctcag ggtaactaca
    28561 ggctgccttt cggcgcgaat acgaacataa agctgcgtgc ttataaaact ggcttcatct
    28621 tcaatccggc gtcggtcgag ttaatttcgc ccggcggaca gcccatcaac ggcgcacgga
    28681 ctttcgattt tacagggacg cggttgccgt tcccgattct catattcgcc ctgccgccga
    28741 ttttgctgac agaggacgaa tcgctcaacg cgctggcgct cgacgccgtg ctgcacacac
    28801 gcgatccgtt cccgctggtt aacgatcatt acttcggagc cgactcgcgc acgcgggtta
    28861 agctgttcct ggtagacctt gatctgtaca gcggcgaaac gctctcgatc atctcggttc
    28921 aggcgcagga cgagcagcgc agagcctacg tcttgcccgt cgaagactta cgcaaagtgc
    28981 ccaacttccc gtggctgtcg caactgacca tacgcttgcc gggcgaactg gcaggcgtca
    29041 cggatataac tctcagcgtc tcggcgcgcg gtcaagccag taacgtaacc cgtctgcgct
    29101 tgagataggc tgttcagaca actaatgctc gtcgtcgggc gcggccagcg gcggagtctc
    29161 ttgcttcctg aaatagcgtg ctccttttag cacctcggca tgcgcttgat gacgactatc
    29221 ctaagagcga agagcaaact ctgaaagagt tcaatccgca tctacgcttt tctatttttc
    29281 cttcaaagga gcaccccgcc atgtttagta gtcggttgag agtcgtgctg ttcgtgctgc
    29341 tgttgccggg cacactgttc gcgcaggaag gcattccttt ctttaccaca gatttcccgc
    29401 ccgaagagtt tgcgcagcgg cgcgctaaaa tttatgacga gatggggagc ggaaagatcg
    29461 ccgtcgtgca gggcgcgccg agtcccgccg ggttcacgcg cttccggcag tcgaatgaat
    29521 tttactacct gtgcggagtc gaatcgccgc acgcctacct gctgctcgac agcacgcggc
    29581 gcagcgcgtc tctctatctg ccgcatcgca acgaggggcg cgagagcgtc gagggtaaag
    29641 ttctctccgc cgaagacgca gagatgatca aacgtctatc cggcgtggaa gccgtctatg
    29701 gctcggaact gctcgcagag catctgtcgc gctacgcccg ctccggacag acgcgcacgc
    29761 tcctgacgcc attcagcccc gccgaggggc tggcgacgag ccgcgacgga gccatccgcg
    29821 ccgtggccga catcgcctcc gacccctggg acgccagccc ttcgcgcgag ggacgtttcg
    29881 cagagatgct gcgcacgcgc tttccacagt ttgacgttca agacctgtcg cccgcgttgg
    29941 ataagctgcg gctgatcaag agtccgcgtg agatcgcgct cattagcaag gcgacgcggt
    30001 tgtcgtcgct cgccctgctg gaagcgatgc gctcgaccgc gccgggagtc tctgaatacg
    30061 agttggatgc cgtcgccaag tacgtctatt accggaacgg cgcgcagggc gatgcttatt
    30121 actctctcgt cgcgagcgga aggaacgcgt ggtatccgca ttacaattca ggcaaacgaa
    30181 agatgctgga cggagacttc ctgttaatgg actacgcgcc tgatgtcggc tactacatga
    30241 gcgacgtgac gcgcatgtgg ccggtcaacg ggaaattcaa cgcgtggcaa cgcgaactct
    30301 acggctttta tctggactgt tatcaggcca tcttgaagca catcaggccg ggcgtcacgg
    30361 cggctgtgat taaacaggat gcgctgaaag atatggagca ggcactcgcg cgcacgcgct
    30421 tctccaaacc cacttacgag cgcgccgcgc gtgagtttgt ggagggctac cggaggtctg
    30481 cggcgaatcc gcgcgcgtcg ctcggccact gggtcgggat ggcgacgcac gacgtgggac
    30541 aagacagcgg gccgctgcgc gccgggatgg tcttcaccat cgagcctgcg ctgcgcgtcc
    30601 cggaagagaa gatttacatt cgcctcgaag atgtcatcgt gatcacggac aagggcgcgg
    30661 atattctgac caactggctg ccgatggaca ttcccgccat cgaaaaaaca atgagcgaag
    30721 agggcttgtt gcagcgttat ccgcgtgacc ggcaataagc ctggcggact cgaatattcg
    30781 ctcccggcgc gcatttgccg cccggctccg gcgaacatgc cgctcaactt ttgtggctcg
    30841 aagtgcatct aaataacgac ctgtagaagt cgggtcgtaa gatagaacag gggagtagat
    30901 cacccggatg ggtctggcga ggccgtcaac acggtcgttc cgattgaagg gagcgacccg
    30961 gcgcgccggg gtaaggatca agaccttttg cgccattttc agatgcgcgc agggggtctt
    31021 tattttttta cgcacagggg agattgacga tgtttaattg gagcatgatc aaaaccacac
    31081 ttttactcgg cggtttaacc gggctgctcg tgatgatggg tggcctcatc ggcggcggct
    31141 acggcgcgat gctcggcttc gtcgtcgcgc tggcgatgaa cttcggcagc tattggttct
    31201 ccgaccgcat cgctttgaag atggcggggg cgcgggaagt ctcttaccgg gaagcgcccg
    31261 agttgcacga catggtggca cggctcgcgc aagctacggg gctgccgaag ccgcgcgtgg
    31321 caatcgtcga gtcagacgcg ccgaacgcct tcgccacggg caggaacccg cagcacggac
    31381 tcgtggccgt gacgacgggc atcctgcgcc tactcgacca gagagaactg gccgccgtca
    31441 tctcgcacga actcggccac atccgcaacc gcgacgtgct ggtgagcacc gtcgccgcga
    31501 cgtttgccgg ggcgatcacg atgatcgcgc agatcgggca gttcgcagtg ctcttcggcg
    31561 gtgcgagcga cgaagatgaa gacggcggcg ggaacattct cggcgcgctg ctcatgatct
    31621 tcctcgcgcc tctggcggcg acgatcatcc aactcgccat ctcgcgctcg cgcgagttcg
    31681 gtgcagaccg gacgggtgcg gagacgggcg gcgacccgca ggcgttggcc tccgcgctgg
    31741 agaaactgga agcctatgcc aagcgcatcc cgctgccggt caaccccgcc gtctcgcacc
    31801 tgttcatcgt gaagccgctg acgggattct ccgtgcaggg actcttcagc acccacccgc
    31861 cgacggctga gcgcgtcgcc agactgcgca tgatcgcgcg tcaaagggtg aatcaagcgt
    31921 aaaggctgcg ggctgcaccg gctactgctg tttcatcgtt cccaccgtga cctgttggct
    31981 cgcgggtcag tttgagattc ttcgccagca tttatgtggc gcgcattttc aaactgaccc
    32041 gctgcgcgca atagaaactt tcttgacatg cccgccacgc agggttagat ttttcgcgcg
    32101 ttcgattcat ttacgagaga cttcactcct cagaagagtc tcgcacctgt tgacctcacg
    32161 ccacgccgca gattaacaag cctcgttaag agccgcgcag ccgccgcctt cacgtccggc
    32221 agaagaggat ggactaataa tggaacccga cccgaagggg atgaatgatt acccgcacag
    32281 cgcgcaggca ggcacaggaa gtctcccgac cgatcttaac gtcccttcgt tccataacac
    32341 gggcgacgtg gtgggggcgg tcttccagct ctaccgcgac aattttctgt cccttctcaa
    32401 gatcgtcgtc gtgctggccg tccctttaat cgtcgcccag cacatctttc taaagctgtc
    32461 gcgcgtccct ctggcggaag ttaccattta cgtgtccgcg attgtcggcg agtcgctgat
    32521 gtcgggcgcg ttgatctacg cggtcgtcac cttcctgcgc acgggggctt tcccgacgct
    32581 ggcagactct tacacgtggg ggcgcaagcg ttggggcagg gtgctgttct gctcctttct
    32641 ctatatgctc gtcgtcgtcg ccgggtgtat ggccttaatc gtccccggca ttatctttag
    32701 tttgatgttc gccctcgttg ttcccgtggc cgtgctcgaa gacaagcctg catcggacag
    32761 ctttagccgc agcagggact tgacgagaaa ctaccgcctg caaatcttct taacgtattt
    32821 cgtcttcagc ctgctcatct tcgtcatttc gctcgttacg aatgcgggct tcggcggcga
    32881 gccggaggac gagatttcgc tcccgttcgc gctcgtacag ggacttatca cccaactgct
    32941 cgaatcttcg tcaaccgtgc tcacgctctt catctatctc ggcattctcc gcgacacgag
    33001 ccaactctcg acgcaattcc cgaacgcccc tccgccccct ccgcccggag acgatccggc
    33061 gcgtgcgtct caagttgaaa ccggtcgtga attttaggta gcctgtcgag acaacgctcg
    33121 acgcaacgcc gctttgcatt ttgcgtgtgg catccgccgc gcggctgaga ttcacgccgc
    33181 aggtcagtgg cgcgtggggg cgcgtcgggt gaagctccga ccgttgtgtg aggccgcttt
    33241 cggacgcggc agtaaattct cgaagacctt gcagtgaagc gaaagagacg atggtttata
    33301 caactcagat tacacgcgcc aacccgacat acgtgatctt cctgctagac caatccggtt
    33361 cgatggccga ccctttcagc ggcgatcaaa tcctgagcaa ggccgacttc gtggccgacg
    33421 tggttaatca cacgctccat gatttcgtca tccgctgcac gaaaaccgag gaagtgcgta
    33481 attactacta cgtctccatc atcggctacg ggcgcaccac cggctcggcc ttcgcgggca
    33541 cgctcacgga tcgcactatc gtccccatct ccgacgtagc cgataacccg caaaggatcg
    33601 agacgcgcca caaacgcgtg cccgacggca gcggcggcgt cgtggaaatg cccgtccgtt
    33661 acccgatctg gctctacccg cgcgcgggcg gcgagacgcc tatgtgcggc gcgttcgcgc
    33721 tcgccaagtc gctcatcgag agatggctgg gcgaacaccc gcgcgggttt ccgccgaccg
    33781 tgctgcacct gacggacggc gaatccagcg acggcgaccc gaccgaactc ggacgacaga
    33841 tcatgtcgct cggaaccgac gacggccagg tgctgctctt caactgtcac gtctcaaccc
    33901 accgccgccg caaagtcgaa tacccgaccg acgatgcttc tttgcccgac gcttttgcac
    33961 gcacgctgtt cggcgtgtcg agtctgctac cggcaaattt tctgggggct gcacagcagt
    34021 taggcgtgaa tgctacggaa ggttcgaggg gcttcgtgtt caatggtgat ccgtcgtccg
    34081 tcgttcagtt ctacgagatc gggacgagcc tgacggggat gacgccgcac atctggatgg
    34141 aatcgtaagc cgcgccgccg accgccgccg cgccccactg ataccgcgat gacctcccaa
    34201 cactggcgca tcgaaactca acacttcctg cttgagaagg aagactccac ccctgcggag
    34261 tgcgaagacg cgatcagcat caacgctgag tcgcggcgtt acgcgctcgc cgacggtgcg
    34321 accgaagggt tcgactcgcg cagttgggcg cgtctgctgg ttgactgctg gacgcaggcc
    34381 gaccccgccc cgctctcggt tgaaactttc cacccgtggg tcgaagaaca ggggcgtcgg
    34441 ctgcacgccg tgtgggctga gcgcgcgctg ccgtggtacg ccgaagagaa ggcgcgtcga
    34501 ggttcttacg cggccttcgt cggcctccag ttcggaggcg gcgacgacgc gctccactgg
    34561 caggccatcg cgctcggcga ttcatgcctg atccagcgac gcggcggcgc ggtctgtcgc
    34621 gccctgccca tctcagacgc gagcctcttc aattcgtcgc ccgtgctcgt gccctctctc
    34681 gactctctac aggcacccgc gctctcccgc gccaccgtcg agcgtggcac agccgaacgc
    34741 ggcgacgttt tcctcctgtt gtccgatgcg ggcgcggcgt ggtatctcaa actctcgaag
    34801 gagcggaagg ccgtcgaggc ggagtttgac gcttcgctcg cggcgtcaga cggcggcgcg
    34861 ctgtcggagt tgttccggcg cgagcgtcag gcgaaacgga tgatcaacga cgacatcgcg
    34921 attctcagga tcgcaatcga accggcacgc cccctttcct gagcgagtgt ttgagcgagt
    34981 gttttatgtc gagcctcacg actcccaatt ggcctagcgc acgacagttc gcggaagctg
    35041 tccaatgccc cgctatctgt ttcgccgagc cgcacattaa aaagttgctg cccgccgtag
    35101 accgtctcgg catgccgctc gtgacttccg ggcagttcgc ttacgtcttc aaactcaagg
    35161 gcgcggacgg gagcacgtcc gtcgccgtgc gctgctttcg tggctacctc ggcgaccgtg
    35221 agaagagata caaagcgatt gacgcccacc tgaacctcta ccagattccg gccttgccgc
    35281 gattcaaata tttcccctac gggatactcg tcgccgggcg caggttcccg gcgctcatca
    35341 tgcaatggat cgaaggggcg acgctggacg tttatctgga agaggtgatt gacaggccgg
    35401 acgtgctgcg gcatctggcg gacgagtggc tcaagctgat gtcgcgtctg cgcgaggccg
    35461 ggatcgcgca cggcgacttg cagcacggaa atatcatcgt cgagcgtgga cagttgcgcc
    35521 tcattgatct ggacgggatg ttcgtccccg ccatgtccgg cttgcaggcc agtgaaatag
    35581 ggcatcaaca ttttcaacat cccaacagag acgcacgcta cttcgacgcg actatcgaca
    35641 acttctccgc gctggtgatc tacctgtcgc tcatctcgct tgccgagcgg cctgaattgt
    35701 ggcgtgagta ccacgacgaa aacctgatct tcaccaaagc ggattttctc gacccggcct
    35761 cgtccacgct gctttcccgg atcaagagta ttggcacgga gcatcgtcaa ctggcggaaa
    35821 ttctacagac cgccgccgtg tccagcccgg tcgagacgcc caacctcgta gacatcgtga
    35881 gtccaaaatc gaagctccct tcgtggatgg ccgcgcccct cgatctggaa gttcaggagc
    35941 ggacgcgcga agcgacggac ttcgagcttg cgcccgtcgt ggctcagaga ctttggcctc
    36001 caccggagac gcagagcgcg gcggtcgccc cggcggttag cgcgtcgccg ggcatcctca
    36061 acccggcgac gcccgtggcg agtctcccgt ctcacaaccc tttggacata cgcgggaaca
    36121 ctctcatgta tgcgcgtcta ctcgacgagc acacctatat tttcctctgg tggttattac
    36181 tgcacaggtg gatcgcaggg ttctgggaac tgttcggcat aggctacttc agcgcgatgc
    36241 tgcttacgct cttgctcctg ataggaacct ttgtggccta cggtttcgtg cgcgccgtcc
    36301 tcgactggaa tctggcttct gacgacccgc tgacctcttt cggctcgccg accgtactct
    36361 ccatcctgca atcctccgct tcgcacgcgc ctgccgcgct cccaccctcc acgcgccccg
    36421 cacgtcatct cacactcaac gccgacgtcc gcgcagtcgt cggcaacagc agtttgcgta
    36481 tctaccatct gcccgattgc atctgggtca agaagatctc gccccatcac agcctacagt
    36541 ttgcgtcgcc cgcacacgcc gacgcagaag gttaccgcgc ctgcaaagtc tgctccccct
    36601 gaacggaaat cctgtcgctc actaacctga caatccggga cttgtcgtgc gttcggcgtt
    36661 cgaaccggag aggcttgtcc cggcggtgag ggaagccgcg cgccagaatg acaaagcaca
    36721 gcccgtcgca gagttcaggg agatggaaca gatcgtcagc gaggtggcgg cctccggtcg
    36781 cgctcagata cgaatagtga gatgattcga tagcgtcgct ttgaggagcc tgtcgctacc
    36841 ttaacgcggg tcgaaaggga aactatatgt gaggcgacct ttaaccttgt gtggccgtcg
    36901 cgccgtgcgg aagggaagaa aaccggcacg gcgcgcggcg tcttccgcca cccgccacag
    36961 ttccctcggc ccctcaattg cgcgagagta aatgacacgc ccggtttcat caatcacgac
    37021 ttcgacggtc acgttaatgg gcgttgtggc ctttctccag ttgatcggat atgcggctcc
    37081 cggcaggctg agagccttat cattgaccag cccaacatcc gtcaactgat cgacttctat
    37141 ggccacacca gccgcctctt cgagtctcgt ttgcgacgcc tctttctccg acgggcgggc
    37201 tgcggagacg ttggccggag cggacgccga ccgtgctcct tttgaactgc tcgccacgcg
    37261 ttcgctcctc ggcagcgcgt ctctacccgt aggcgtctga gaggccgcgc tctcctcttc
    37321 ggctacgggc agcgcgccgt caacgcctcc cgaataataa gtgatagtgc gataggcgac
    37381 ctcgaccggc tcaagccgct gctggccggc ttctgagacg acgacagagg aagtcatctt
    37441 cacccaattg cccgccgcat cgaattcata ggagtattca gtccggctga gaatgacacc
    37501 ctgctcatcg cgcaccgtag ttctcaacaa gcgtcccgcc tcgtcgtaat catacgtctc
    37561 ctgaccgatg gggctattga tcacgggata ggttctgtta tcgatccgtc tgccgcttat
    37621 atcatagaca gtaagttcaa gcagggaacg gggtccttcc accactccgt tcttcttgag
    37681 gttaactctc gccacctcgg ttcgcaggcg gcgcaccagc ccgcgcagcc cgtctctctc
    37741 tctgttactg ttaaggattt tgagcggcga aggactccgg cgttggtcag ttcgcgtctg
    37801 cgcgagaagg ctctgactga gagaaagcag gcagacgact aagagcagga cgtgggggaa
    37861 atgtctgacg atgaatctgt gataattcat ggcggagccg ggctgtcaca ctggccgacg
    37921 gataaagttc taactggtgc aatcgacgtt cgcggcgacg atgctcgtat ttacgccgcc
    37981 ctgtgcgttg aggctgatcc gaaatttaac gtgtcccatc aagccgtcag tacttcccgt
    38041 tttgttcagc gcgaagttga agacataggt gaagctggtg ggcgaaggac cactcgattc
    38101 tagacgaccg acgccgacgg cctgatactt agcgcggctt gtgagtccga aagcgttaat
    38161 ccgatccgcg ttgatgttgc cctcgatcaa tgtctggccg ccggcctcgc ggacgacctt
    38221 gtactgcaca tcgaattcac cgctcaggcg aatccttttc tctctacacg ccgtgggcag
    38281 ggtgcgattg aatggaatct tccgggcacc ggaaacggga gggttttgcg cctgtgcccg
    38341 cgccactccg aaggctgcga gcagaatcat agccgttgag gccactgaga agatcgagag
    38401 ccgcgttatt cttttgttca tgagagagta gtgtggcaaa aaatttctgc ccgcacaaga
    38461 tatttaatgc agccgcgtac tctccagcac aactgacggc gggaaaagat attttgccgc
    38521 actgccaatc tcttttgaga agaagagcga acaggggtgt gcgcgttcaa cgcgataagg
    38581 cgagcaccgc cgcgtagact ccctgcacaa ccatcgccat gcgcttgtag tcgagcgtgt
    38641 cgggcgtgtc ggtgaaagtg tgatagttcg gattccggta aaaagccgtg tcggtgatca
    38701 tcaacgcgtc gtagccctcg ttccagtagt tcagatgatc ggagaagtct acgccgggaa
    38761 tcgtgcgcgg cgcgttaatc gaatagaccg gaagcggcga cgcttccagc atcgctttct
    38821 tcacgcgtct catcgtccgc acctgatcca tctttcccac cacagagatg aaattcccgc
    38881 gcgcgggata aaaggctttc aggatggaga cggggtaatg ctggctctca ggctgatcgc
    38941 tgaaataacc gatcatctcc aaagagagca tcgcgcgcac gttgaagccc tgcgccctga
    39001 gtgaagaggc gtggatggcg ctgcccatca gaggagaacg gaagtagggc ggctcttcca
    39061 gtgtgaacgc gaccaactcg acacgcactg cgggcggtat ccggccaagc aggtaggcca
    39121 gttcgagcaa gcctgcaacg ccgctcgcat tatcatccgc accgggaagc gcacccgccg
    39181 tgtcgtagtg cgccccgacc acgatcagtt ctttgtcacg gggaccaaag gacgcgatca
    39241 cgttacgata agttcgccca cgcgactcga agggttgctc cgacacttcg atggccttcg
    39301 cctgctcaaa ctccttccgc aggtaagccg caacgcggtc gaggttttcg ggatgccccg
    39361 cgtcgcgcgg gctgagcgtg ccggacagca cgcgcacatg tgcttcgagc cgcgcggcgt
    39421 ctacaggcgg aacgttaccc cccgcagtcc cgcgagagat caagggctgc gtgatcgcaa
    39481 accatgtgac caacagcaga cccgcgaacc ctaaaatgat taacagcaac agcacgacga
    39541 agcgtttgcg cctttcagga tgaactggcg aatttgaaag cgacgtgccg gtcataaact
    39601 agaaacaagc gctctcgccc gcacgggtct ttgccctctc cgaactatga cgcgatgcgg
    39661 ctgcttttca cttgagcggt tcgaccgcac tcgtcacatc gagatgtatg acggcgtcga
    39721 attgcttcgc gaggcgcgcc ttgaagtagt ggctctgccg ctccgtctgg ggcaggtaga
    39781 cgaccccgat ggcacgctca aggtgcggct ccgagagcgc gtcggagact gcgccgcccc
    39841 cgcgaagtat gagcagaaag ttgggcacgc ccgtctggtg aaacagaccg gaatagcttt
    39901 ccgcgagcgc ggggcgcacc ttcatcttct ggccttgcct gccccactcc aaggccgcac
    39961 gcacctcgcc cgtgtaggtc gtaaacccga cgaggacgga tgcgccgtca tgaaatttcc
    40021 gcatgaggtg gccgacgttc aactctccgg cctctcccat ttcggtcatg cgcgcgtcgc
    40081 cctgatgtgt gttgtgcgcc cagacaacca acttcgcctt ctctccgccg atggcttcct
    40141 cgtaacggct gatctcttgg agcgtctccg ccatgtggct gtcgcgcaaa ttccaggtgt
    40201 tgaagccgcc cagataactc aggcgatagt atgcctcccc gttcttcacc acgcgggcgt
    40261 tctggtaggc cgagaagaga tcgtcgtcgc gctccggctt cggtcgtgcg cgccacgcgg
    40321 ccagacgctg ctccatctcc tgaaacagcg caagggcgtc tttctcgcac gaagttttca
    40381 tcgccgtcgc tacctcctgg ccgtaccgct ccggacggtc gccgtagcga cggaaacact
    40441 ccgtgcgctg ccgtgcacgc gtagcggctg ccgggtcaac gcgcgtcagg tattcgacca
    40501 caacctgcga agagtctgag aggttataca ggtccatgcc gtagacgcct gcgcgtgggg
    40561 catcggaggg tcgagaggcg ttatgagagc ggagccatgt gacgagatcg cggaactcct
    40621 cgttgcgcca catccactgc ggaaagcgcg tgaagccgga gagcgcctgc tcgacgttcg
    40681 cgtccttgcc gcgcccacgc acgtactcgc tcacgcggta agcgtccgac cagtcggctt
    40741 ccagcacgac ggcgttgaaa cctttctcct cgatgagacg gcgcgtgatg cgagcgcgct
    40801 cgcggtagaa ctcttgcgtg ccgtgcgtgg cttctccgag caggacgaac cgcgcgtctc
    40861 cgaccaaatt catcagcgca tcgtagtcgc ccttaccgcc ggtaagcggt atcgccgatt
    40921 tacgcaccag cgcagagatt gaagcgactt tatcgacgga cgcggctgcc ccctgcgctt
    40981 tgccgcgccc ttgttcttcc ttaatcccgg acatgcagcc attagctgtt aagcagacaa
    41041 acagcattgg ccgcagcaac ccgagaagag tacgctggca catgaaacct ccgcagcatc
    41101 aaactccggg caaagtgaga acgggcttgc gcttgcttac atgattggct acacgcaact
    41161 cttctcaaac acttcgacgg atgaaattgt taggggagga taaatatagt cggacgacgt
    41221 gagactgatc tgtaccaagc cgtactttat agaaggaact caagccagca tacgataaca
    41281 acaccgacga gtccgataac gcctgctcaa agcgttgggg ctatgaggca aaagtttttc
    41341 gctggtacaa cgggaacatg gagggcattg tcagttgacc gtgctcgaca tatccaaacg
    41401 ttccaaactt ttgcctcata gccctacgac ataatttttg ttatcggact ctaactgttt
    41461 atggtatcgg ttggcgttgc gcttcgagcc agcgttcgag gccgatggcg gccagtgtcg
    41521 cgctcatctg ggagacgtat tgcgcactta ctcgctcgac catcattcgg atcacccgag
    41581 cgccgccgag gcaccaaaaa aagtggcggt caggcggcac aggtcgggcg gcgaagtcat
    41641 ggcggagcag gatgaagaaa gcgcgagcac ggattatgac taagaggtca ttcctgcaac
    41701 gctatcctca tgcggcggac aaatcgactg ctcccctgtc gaacctcgaa tcaatcgtct
    41761 ttaactggag cgccggagtg gggctgagat attgtggcgg gatactgagt tcctccatct
    41821 tccggcggtc ggctgcttct atcgggtcga gcccggcgat gtagcgccgc tcattgtttt
    41881 gcaggggatt gctgatgctc aggatttcgg aacccgaagc gagaatcact tttttgtact
    41941 gaggcaggtt gctcgcccag agttttgtcc agtagtggcg tccgacgttg gtgaggctgc
    42001 cgagttggcg gcagagcctt ttgaaatgtt cgaccgcctc ggtcggttcg agtccgaggg
    42061 ggcgatggca gagcgtgtag ccgtcgtagt cgcggctcac agtacccgga agaatcctgc
    42121 cctcactttg cagttgccgg taaaagggcg tctccgggta agggcagacg atgccgagaa
    42181 aagtgatatt gaagtattga aggtctgaca gatactccgg cagtttctcc agatactcgt
    42241 tcgtgtctcc gtcggagccg acgagcaggc cgaaggagag catgatgccg ttggcaaagc
    42301 agcggcgaat cacccggtca acttccgtga tcttgttctg ccccttgttc atcgccttga
    42361 tggagtcggg gttgagcgat tcgaggccgg tgtagatgta gcggcagccc gcctgcgcca
    42421 tcaacttcac caactcttca tccttgagca cgttaaatgt cagcgcgcag ccccatgtct
    42481 tcttgagcgg caggagcgct tcgcaaagcg tgcgcaggta gcgcgggtcg ccgccgagat
    42541 tgttgtcgag gaacgagaac gaattatcga acaggccgaa gtagttcttg ttccaacgca
    42601 tcttgaactt gatttcgtcg ataacgtgct cgacggggcg atagcgatat ttttcgtggc
    42661 cggtgagcac gcagaagttg caggagaaag ggcagccgcg cgaggcttca atgccgggaa
    42721 gcctgatgga gttggtgcgg aagtcgatca agtcgtaacg gtagggcttg atctcgactg
    42781 cgccgcgcga aggcagttga tagcgtttct gtaactgtcc gcgagcgaag tcagc
//
LOCUS       DASWNS010000223         7329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_192326, whole genome shotgun sequence.
ACCESSION   DASWNS010000223 DASWNS010000000
VERSION     DASWNS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192326"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..3807
                     /locus_tag="VGB76_07670"
     CDS             349..3807
                     /locus_tag="VGB76_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0098816.1"
                     /translation="MSYKLNLAPRLGAIFISLFACQFLFSVVSTVVYAADGCSVPSFR
                     PAPFYKTGEAPRSTATGDFNGDGRADFVVANYGNDNVTVFINDGGGAFTVSKNFSVGG
                     GPSSVATGDFNSDGKADIVTGNNTSGNVSILLGDGGGNFATASHFNAGVGMHGVTVGE
                     FNGDGKSDLAVALEGSNRVAVLLGNGAGGFGAPTSFVLGTQQQFPRSIANGDLNGDGK
                     ADLVVGGGGSIQILLGNGTGTFTRVTTACTNNNSDSIVLVDMNGDGKLDLVGTRTSTT
                     NVAVSRGTGTGCFEAATIYNVSPAPAAAHSPYAVAVADFNGDAMLDVATANGGSNNVS
                     LLLGDGAGGLSASNVYGGGRFPRSLAAADFDGDGKADLATAFSEGGNLSVMFGSATSG
                     FRFATGRGTVAALVADFNNDGKSDLAVTSGAFNSSGVIIMLGDGTGNFAGGKKFPAGN
                     TSVSLSVADFNHDGKADVVVAGRGPFPAEVSGVFVLLGDGAGNLGAPAMFGVNANPFS
                     VNVGDFNGDSHTDIVSANLSGNDISILFGNGQGGFGVAASFPAERFNNPQKIAVGDFN
                     GDGKADLGLPTASGVTVLLNNGSGGFPHRVTFPAGSNTQSVITKDFNSDGKLDLAAAN
                     AGSGNVSLLLGDGAGGFGAAFNFPVGAGNPGSGPVDIVSGDFNLDGILDLATANIITP
                     VGAVFYETSASMMLGNGTGGFAAPSFFPIGLSPWTINAGDLNADGMTDLLTANNSSDD
                     ISVLLNSCNAAPPPTSPALVEFGLGDYLVSENFARAAVTVTRSGNASDPLTVTLQTVD
                     DPAAVPCDPTLKRPDGTSYPQGTAYARCDYATTIATVTFAAGDSQPKTVNVPLVDDAH
                     VEGTETVQVKLLNPTGGVLGTRSTATLTIIDNDTTAGRPNPIFSTPFFVRMHYLDFLS
                     REPEPGEPWSAILNGCANQFNTDPQSPSAACDRTIVSQSFFGAPEFRLKGLFVYNFYR
                     VAFDRRPEYAEIIPDMSGVTGATSAEVYAKRAALPVSFTERAEFKARYDGLTDTAYVN
                     ALLDRYGLQQITTPDPQQPEAGVKVVLTRAELVSRLSAAAGTAQALTRAQVLRATVES
                     DEVVAVEYKGAFVAMQYYGYLRRTPEESGYQAWLRVITEDPNNIRIMVNGFVNSTEYR
                     IRFGRP"
     gene            4122..5879
                     /locus_tag="VGB76_07675"
     CDS             4122..5879
                     /locus_tag="VGB76_07675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0098817.1"
                     /translation="MIESGTLLQNRYRVTKQIGQGGMGAVYIATDERFSSTVAVKQTF
                     FDDPALRKAFEREAHLLNHLRHTALPKVSDHFTEGDGQFLVMEFIEGSDLSELLQQRN
                     GAFPVADVLNWADALLDALDYLHTHEPQVIHRDIKPQNLKLTPRGQIVLLDFGLAKGN
                     STQTRANLTASVFGYSRSYAPLEQIQGTGTDVRSDLYSLAATLYHLLTGAAPVDALTR
                     ASAIINGQPDPLRPAHLAHVQVPAAVGEILRRAMSQNAAHRQPTAAEMRAALRRAANA
                     LPASARQAAPVVAPQAARSSTDMPTMLEATPLPTYGQQPQSPTMETRATTQLRQPSAT
                     HQSETTIVDGAAYGARRRPSTNSFVPLSPDATPERKSKAVFIGVAVAVLVACALVAFP
                     LMRRSPATTIALPDASGAQVETPLSSPTDAQSTAPGASQSEATQATNGASSGAETTNA
                     QAGTRTTRPADESGTANGQTANREGGATNGSDASGVGNNTGGDAANVASPVGGGSAGR
                     SDGLVLHAPAPGASSGRSAAAEYDAQRAEDLRKRQQQQQQQLQQQQRPHPPQHEEEHF
                     RPPPPGGGHRPPPRRPPPF"
     gene            complement(6168..>7329)
                     /locus_tag="VGB76_07680"
     CDS             complement(6168..>7329)
                     /locus_tag="VGB76_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0098818.1"
                     /translation="EMSRTLGSVFDLDLIFEKATDIIFRVTPAARVVALLRDERGDTE
                     SGLRPVAMRVRDEKLTATANSLTIGRTITRKVMQERVALLSQDVAADEQFAGVNSIIA
                     QGVRSTMCAPLVGESAVHGALYADCLDQITAFTRDDLELLCAITAQTAVAVENVRSHE
                     RLAREEVARANYSRFMPEYVVRQMLDHPESFKLGGVNQNITVLFADVRGFTRLAEHAP
                     PEQVVQLLNHYFSAMSDIIFAHSGTLDKYIGDGLMALFGAPATTPDDTFNAVTVAIRM
                     QHRILRLNEEFLASGLPEVSIGIGLHTGEATVGYIGSERRTEYTAIGDTVNLASRLES
                     NAGGGQILLSEATASVLAPGQFPLREHAALTVKNRAQPVQVYEVLWQQVDQQ"
BASE COUNT         1411 a         2370 c         2187 g         1361 t
ORIGIN      
        1 ctgcctcatg gagggtaaaa cgtgctggta ggtgtccgag ttaatccgtt aaaaccggga
       61 ccgtaatcat taaccgctgg tgaccgtaag cctcgcctgc ctcagtggca tcgcctaaat
      121 tcagacgggc tggacaataa aattgattct ctgccaacca aacatcttcc gagccgtttc
      181 ttgactaatt tcatcccgct aagctatacc taccaccctg cgaccagaac ggcagaaact
      241 tggaagcttt actctccggc acgccactag agtagttaag tatcccccaa ctcttcaggc
      301 agtcttaatg aaataatctc cccgtccgtt actcacgaag aaactcttat gtcttataaa
      361 ctcaacctcg caccacgtct cggagccata tttatttctc tgttcgcgtg ccagttcctt
      421 ttcagcgtgg tatcgactgt agtttatgcg gcggacgggt gctcggtgcc gagtttccgc
      481 cccgcgccct tttacaagac gggggaggca ccgcgctcga ccgccacagg cgatttcaac
      541 ggcgacgggc gggctgactt cgtcgtagcg aattacggta acgacaacgt caccgtcttc
      601 atcaacgacg ggggcggagc cttcaccgtg agcaaaaatt tctcagtggg cgggggtccg
      661 tcatctgtag cgacggggga ttttaacagc gacgggaagg ccgacatcgt gaccgggaac
      721 aacacgtccg ggaacgtttc aatcctgctg ggtgacggcg gcggcaactt tgccacggcc
      781 tcccatttca acgccggagt gggcatgcac ggagtcactg tcggggagtt caacggggac
      841 ggtaaatccg atttggcggt ggctctggag ggcagcaacc gggtcgccgt cctgttgggc
      901 aacggggcgg gaggcttcgg tgctcccact tccttcgtct tagggacgca gcaacagttt
      961 ccgaggtcta tcgccaacgg cgatctcaac ggggatggta aggccgacct cgtcgtcggg
     1021 ggcggcggct cgatacaaat tcttctgggc aacggcacgg gaacctttac gcgtgtcacc
     1081 accgcctgca ccaacaacaa ctccgactcc atcgtcctcg tggatatgaa cggtgacggt
     1141 aagctcgatc tggtcgggac gagaacctcc accaccaacg ttgccgtctc gcgcgggacg
     1201 gggacgggat gttttgaggc ggcgacgatt tataacgtct cccccgcccc ggctgctgcc
     1261 cacagccctt acgcagtcgc cgtggccgat tttaacgggg acgccatgct tgacgttgcg
     1321 accgccaacg gcgggtcgaa caacgtctcg ctcctgctcg gcgacggcgc gggcggattg
     1381 tcggcctcga acgtctacgg cggggggagg ttcccgcgct ctctcgcggc ggctgatttc
     1441 gacggcgacg ggaaagccga ccttgctact gcattctccg agggcggtaa cctctcggtc
     1501 atgttcggca gcgcgacaag tggcttcagg ttcgccaccg ggcgtgggac ggtggccgcg
     1561 ttggtcgcgg attttaacaa cgacgggaag tccgatttgg ccgtcacctc tggcgccttc
     1621 aactccagcg gcgtcatcat catgctcggc gacgggacgg gcaacttcgc cggcggcaag
     1681 aagtttccgg cggggaacac ttccgtctct ctctctgtcg ccgacttcaa ccacgacggc
     1741 aaagcggatg tggtggtggc cggcagaggg ccgttcccgg cggaggtcag tggggtcttc
     1801 gtcctgttgg gcgacggcgc ggggaacctc ggtgcaccgg ccatgttcgg cgtcaatgcg
     1861 aatcctttct ccgtcaacgt cggcgatttc aacggcgact cacacaccga tattgtgtcg
     1921 gcgaatctaa gcgggaatga catctcgatt ctcttcggca acgggcaggg aggcttcggc
     1981 gtagccgcta gcttccccgc cgaaagattc aacaacccgc agaagatcgc tgtcggcgac
     2041 ttcaacggcg acgggaaggc cgacttggga ctcccgaccg cgagcggcgt caccgtgctc
     2101 ctcaacaacg gttccggagg ctttccccac agggtcactt tccccgccgg cagcaacacc
     2161 cagtcggtca tcacgaaaga cttcaacagc gacggtaaac tcgacctcgc ggcggccaac
     2221 gccggttccg gcaacgtttc gctgttgttg ggcgacggcg cgggcggctt cggcgcggcg
     2281 ttcaatttcc ccgtcggcgc gggcaatccg ggttcggggc ctgtggacat cgtgagcggc
     2341 gacttcaatc tcgacgggat tctcgacctc gctaccgcga acatcatcac gcctgtcggc
     2401 gcagtctttt acgagacgag tgcttcgatg atgctcggca acggcaccgg cgggttcgcc
     2461 gcgccctctt tcttccccat cggactcagc ccctggacca ttaacgcggg cgacctcaac
     2521 gctgacggga tgacagacct gttaaccgct aacaacagtt ccgacgacat ctcggttctg
     2581 ctcaactcct gtaacgcagc accgccgccg acatcacccg ctctcgtcga gttcggcctc
     2641 ggcgattatt tggtttccga gaatttcgct cgcgctgccg tcaccgtcac ccgctccgga
     2701 aacgcgtccg acccgctgac cgtcacactg caaaccgttg acgatccggc ggcggtgccc
     2761 tgtgatccga cgctcaagcg cccggacggc acttcgtatc cacaagggac ggcctacgcg
     2821 cgttgcgact acgcgaccac tatcgcaacc gtgacatttg ccgccggcga ctcacagccg
     2881 aagaccgtca atgtcccgct cgtggacgat gcacatgttg agggaaccga gacggtgcag
     2941 gtcaaactgc tgaaccccac gggaggcgtg ctcggcactc gttcaaccgc gaccctcacc
     3001 atcatagaca acgacactac ggcagggcga ccgaacccga tcttttcgac gcccttcttc
     3061 gtgcggatgc actacctcga cttcctctcg cgtgagccgg agccgggtga gccgtggtct
     3121 gccatcttga atggctgcgc caaccaattc aataccgatc cgcaaagccc ctcggcggca
     3181 tgtgatcgga ccatcgtctc gcagtcgttc ttcggcgccc cggagttcag attgaaaggc
     3241 ttgttcgttt acaacttcta ccgcgtcgcc ttcgaccggc gacccgagta cgccgagatc
     3301 atccctgaca tgagcggcgt gacgggcgcg acatccgcgg aagtgtatgc caaacgtgcc
     3361 gcactgccgg tcagcttcac ggagcgtgcg gagttcaagg cgcgctacga tgggctgaca
     3421 gacactgcct acgtcaatgc gctacttgac cgctacgggc ttcagcagat tacgacgccc
     3481 gacccgcagc agccggaggc gggcgtcaag gtggtgctga cgcgagcgga gttagtaagt
     3541 cgattaagcg cggcggcagg cactgcgcag gcgttgacgc gtgcgcaagt gttgcgcgcc
     3601 acagtggagt cggatgaggt ggtagcggtc gagtacaagg gagcgttcgt ggcgatgcag
     3661 tattacggtt acctgcggcg gacgccggag gagtcgggct atcaggcgtg gctcagggtg
     3721 atcactgaag acccgaacaa catacgcatc atggtcaatg gtttcgttaa ttccaccgag
     3781 tatcgcatcc gtttcgggcg gccttgagac gacgccggag cggttggttt aagataataa
     3841 cgccctgaaa gagcgagctt tcagggcgtt attgtttttc cgtctgcggc tggtaaaaag
     3901 ggcgcatcgt tgtgccgacg ttgaagcgca gaatgcgttg tgcggacgtg gcgaaaatag
     3961 gtgttgatta aagtaagagc tataagctaa tctggatact aagggcgaaa ccctctcgca
     4021 agacgtttcg cgcccgccct gcttgcccgc ctgacagaag ttcaatcctt tcagcatcta
     4081 acccgccgct tcccgcgcga aggagagtcg aaaagtctat catgatcgaa tccggcacac
     4141 tgctccaaaa ccgttaccgc gttacgaaac agatcggaca aggcgggatg ggcgcggttt
     4201 atatcgccac ggacgagcgc tttagcagca ccgtcgccgt caagcagact ttctttgacg
     4261 acccggcctt gcgcaaagcc tttgagcgcg aggcgcatct cctcaatcac ctgcgccaca
     4321 ccgcgctccc caaggtcagc gatcacttca cggagggcga tgggcagttc ctcgtcatgg
     4381 agttcatcga aggctccgac ctctccgaac tgctccaaca gcgcaacggc gcgtttcccg
     4441 tcgccgacgt gctcaactgg gctgacgcac tgctcgatgc gctcgactac ctgcacacgc
     4501 acgagccgca ggttattcac cgcgacatca aaccgcagaa cttgaaactg acgccgcgcg
     4561 gccagatcgt gctgctcgac ttcggactcg ccaaggggaa ttcgacgcag acgcgcgcca
     4621 acctcacggc cagcgtcttc ggctactcgc gcagctacgc gccgctcgaa cagattcaag
     4681 gcacgggtac ggacgtgcgc agcgacctct attcgctcgc cgccacgctc tatcatctgc
     4741 tcacgggggc tgcccccgtc gatgccctga cgcgcgccag cgccatcatc aacggccagc
     4801 ccgacccgct ccgtcccgca cacctcgcac acgtgcaggt tcccgccgcc gtcggggaga
     4861 tactccgccg cgcgatgtca cagaacgcgg cgcatcgcca gcccaccgcc gccgagatgc
     4921 gcgccgccct gcgtcgtgcg gcaaacgcgc tacccgcgag tgcgcgtcag gctgcgcccg
     4981 tcgtcgcgcc gcaggctgca agatcgtcaa ccgacatgcc cacgatgctg gaagcgacgc
     5041 cgctgcctac ctacggccag cagccgcagt cgccgacgat ggagacgcgc gccacgacgc
     5101 aactgcgcca gccttccgcc acacaccaga gcgaaacaac catcgtagac ggcgcggcct
     5161 acggcgcgcg ccgacgccct tccacgaact ctttcgtacc gctctcgccg gacgcgacgc
     5221 ccgagcgcaa gtcgaaggcg gtgttcatcg gtgtcgccgt cgcggtgctt gtcgcctgcg
     5281 cgctcgtcgc tttcccgctc atgcgccgct ccccggctac cactatcgcg ctccccgacg
     5341 cttccggcgc gcaagttgaa acgccgctct cctcgcccac agatgcacag tcaactgcgc
     5401 cgggcgcgtc gcagtccgaa gccacgcaag cgaccaacgg cgcttcttcc ggtgcggaaa
     5461 cgacgaacgc gcaggccggg acacgcacca cgcgtcccgc agacgagtcc ggaactgcta
     5521 acggccagac ggcaaacagg gaaggcgggg cgacgaacgg ctccgacgca agcggggtcg
     5581 gcaacaacac cggaggcgac gcagcgaacg tcgcgtcgcc cgtcggcgga ggatcggcgg
     5641 gtaggtctga cggcctggtc ctgcatgcgc ccgcgccggg tgcgtcgtca ggtagaagcg
     5701 cagccgccga atacgacgcg cagcgcgcag aggatttacg taagcgacag caacaacagc
     5761 aacagcagtt acaacaacag caacgcccgc accctccgca acacgaagaa gagcatttca
     5821 gacctccccc gccgggcggc ggccaccgtc ctcccccgcg tcgcccgccc ccgttctaag
     5881 aaactgttag cgacgtttca tccgcgacat aggatagaaa gaagtcccgc agggacgatt
     5941 gaaagttctt ccggataact ttcaatcgtc ccggcgggac tcaacttttt aacttcctgc
     6001 attgtccccg cactcagatg tcgaactgtc agcaaccgcc ccggcacgac ctgtcaggcg
     6061 gcttctaacg agcgcgtcat gccttcatcc gatttgactg agtaacgggt gcgccgtgga
     6121 gacgcgcggg cttaaaacgc gtgaggcgcg cgggcaactt cgattcgtta ttgctggtcc
     6181 acctgttgcc ataagacctc atagacctgt acaggctgcg cgcggttctt gaccgtcaaa
     6241 gcggcgtgct cgcgcagcgg aaactgcccc ggcgcgagca cgcttgccgt cgcctcgctc
     6301 aataaaatct gcccgccccc cgcattcgat tccagacgcg acgcgagatt tacagtgtcg
     6361 ccgatggccg tatattccgt gcgtcgctcc gagccgatgt agccgaccgt tgcctcgccc
     6421 gtgtgcaaac cgatcccgat gctgacctcc ggcaagcccg acgcgagaaa ctcctcgttc
     6481 agtcttagaa tccggtgctg catccggatc gccaccgtga cggcgttgaa ggtatcgtcc
     6541 ggcgtcgtcg ccggtgcgcc gaagagcgcc atcaggccgt cgccgatgta tttgtcgagc
     6601 gtgccgctgt gggcgaagat gatgtcgctc atggccgaga agtaatgatt gagcaactgc
     6661 accacctgtt ccggcggcgc gtgttcggcg aggcgcgtaa agccgcgcac gtctgcgaag
     6721 agcaccgtga tgttctggtt gacgccgccg agtttgaacg actccggatg atcgagcatc
     6781 tggcgcacga cgtattcggg catgaagcga ctgtagttgg cgcgcgccac ttcttcacgc
     6841 gcgaggcgtt cgtgcgagcg cacgttttcg acggccaccg ccgtctgcgc cgtgatcgcg
     6901 cagagcagtt cgagatcgtc gcgtgtgaag gccgtaatct ggtcgaggca atcggcgtag
     6961 agcgcgccgt gcactgcgga ttcgccgacg agcggcgcgc acatcgtcga gcggacgccc
     7021 tgcgcgatga tcgaattgac gcccgcaaac tgttcgtctg cggccacgtc ctgagagagc
     7081 agggcgacgc gttcctgcat cactttgcgc gtaatggtgc gtccgatggt caggctgttg
     7141 gctgtggcgg tcagtttttc gtcgcgcacg cgcatcgcca cagggcgcaa gccgctctcc
     7201 gtgtccccgc gctcgtcgcg cagcagagcc acgacgcgcg cggcgggcgt gacgcgaaaa
     7261 ataatatccg tagccttctc gaagatgagg tcgaggtcga acaccgagcc gagagtgcgg
     7321 ctcatctcg
//
LOCUS       DASWNS010000224        15799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_372149, whole genome shotgun sequence.
ACCESSION   DASWNS010000224 DASWNS010000000
VERSION     DASWNS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15799
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372149"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VGB76_07685"
     CDS             <1..1380
                     /locus_tag="VGB76_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0098819.1"
                     /translation="NFLTSILPATTPRLNEIGIDVWVLGFTFFISLLTGLVFGLAPAL
                     KASKLNLIEALKDRVQGAAEGLRRNRVRSLLVVSEIAISLIILIGASLLVKSFWRLQE
                     VDAGFNPANLLTLQVSLPAAKYSEPAKQRAVFQEIFQRLESLPGAQAVGISNDFPLAG
                     NMQTSQPYIEGRVTIPGQESLSGVHAVSHNYFQAMGIPLLKGRAFTASDIESAQPVMI
                     INKMMAQRLFANEDPLGKRVKFSDDPNVPWNEVVGVVGDVRHNGLDIEPQMETYLPYP
                     QSPRTIMAIAVRSTSDPTAMTSAVRRAILEVDNAQPIYDIKTMEQRLSEAVAPRRLSM
                     ALFSFFAAVALVLAIVGVYGVMSYSVNQRTHEFGIRMALGAQHTDVLKLVLRQGVILA
                     IAGILIGVIAAYAVTRFMSGLLYGVSATDPLIFIAVSLVLMIIALLACYIPARRATKV
                     DPIIALRYE"
     gene            1770..2072
                     /locus_tag="VGB76_07690"
     CDS             1770..2072
                     /locus_tag="VGB76_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098820.1"
                     /translation="MATLPVPERQLFWRNFDIQYHAAHPGLRHMKNNLWELPHWMHWL
                     GGVLVHEGFPLLDVIDFYTSETAFSGINRTKVREVLSEHPADVFLFSPMTPNLPFA"
     gene            2096..2584
                     /locus_tag="VGB76_07695"
     CDS             2096..2584
                     /locus_tag="VGB76_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098821.1"
                     /translation="METGNQESQDLYKQRVKLDPIEETLMKLRDEGIATCSFMVNGFP
                     NQTIDDMKRSIEWVCTLIAKDLLQASYLFGLVPYPGSEMFEHPEKFGMTLHHKNFKLY
                     HEEMPPVFDSPYAKSDAVYEQFLSGVKNLGQAMSKKAYFGEIPPPTELSLYGTFWSDP
                     HV"
     gene            complement(2945..5566)
                     /locus_tag="VGB76_07700"
     CDS             complement(2945..5566)
                     /locus_tag="VGB76_07700"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0098822.1"
                     /translation="MRGYRLSIRMLSLWLVFSFMFVNVPAQSRGAGDASLKVSVVDQN
                     GAVVAAARVRIEMGVGAEQALKTDERGGVVFQGLPAGRVLLEVSAEGFETRIVEGFSL
                     NAGGNQTQVRLEVATVKEEVVVGQDEQERSTDPRGDAFSNVLTAEQIASLPDDPEEFE
                     NALNALAGPGATIRVNGFRGGKLPPKSQIREIRFRRNAYAAENHERGFIAVDIFTKPG
                     VNDWHGSLSFGFRDEALSARPAFASRRGAEQLRRFGFTLDGPLWRNHTSLFLNVDSTE
                     NYDSKTIVAALPGGFFDDLIQRPSRRLNIAARVEHALDKTHSLRLEYQRNAGRQDNLG
                     VGDFDLAERAYTLDQSEQIFRLSESGVFAKRFLNEFRFQARWRTRETLPLSNAPTLLV
                     LNAFTRGGAQTAGQHQTLDFELADNFDLSFGQHSMRAGVLFEGGSYDSTESVNPNGTY
                     TFANLEAFNAARPTTYTQRTGEAALNFSQYQFAWYWQDDYRLRKNLLLSYGVRHELQT
                     NLSDRSNFAPRFGVAWTPNKSGTVTIRAGAGIFYDWFEAGTYEQALRVDGRRQRDLVV
                     QNPGYPDPLSTGTPIILPPGRIQVDPLLKQPTIMQGSLAVESRLFKRFRLNTDYQFQR
                     GQHLLRARNLNAPVQGFGRPNPTAGNITQIESSANSTSHRLSVHFAPAIFTSNLFWST
                     FYVYSRNISDTSGALSLPADNFNLRGELGPAPNDMRHFFSAMVNRKLFKGFSLGATFN
                     ANSALPYNITTGFDDNGDTVSNDRPAGVGRNSARGKGRWDVSTRLGWSFGFGGVREAS
                     ASAGPVMIRMGDGGVPSLPGASNQKFRMEFYAQAFNVFNHTNLSNFTGVQSSPFYGLP
                     TAALPGRRMEFGTKFSF"
     gene            complement(5613..6542)
                     /locus_tag="VGB76_07705"
     CDS             complement(5613..6542)
                     /locus_tag="VGB76_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098823.1"
                     /translation="MQRFINFACLLLLFTAGSFNALAKGNGDAARALEILKQARAAIG
                     GEEKLGSIQSLTLKGKFRRVIQEREMAGEREFNFLLPDKFLKSDTMIVGGSSTSITTY
                     RALNGHEHWTGGGGGRGGMFIIGGNSQKPSPEHLEQMEREQAQRFRAEYARYVLALLL
                     SAPSGYSLEFNYAGEAVADDGRADVIDATGAEGFAARLFLDRETHLPLMLTYRAPKPR
                     MFTTMMRSNGGDKKKPEEIIKEAREKAAQEPAPAKPEEVEMQLRFADYRKTGGLMLPH
                     RITQGSEGEVNEEWEIKTYEFNPQFKADKFQKK"
     gene            6911..8251
                     /locus_tag="VGB76_07710"
     CDS             6911..8251
                     /locus_tag="VGB76_07710"
                     /inference="COORDINATES: protein motif:HMM:NF039204.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HEY0098824.1"
                     /translation="MISGAIIFVNAARQPSRVLLLLLLLLTLALLVGCRRDRRDAVMP
                     VPVTVPPALVDPWRKAALEVEEDRGEPVGRQAKVEVPAQLKHYDNPRRFLGIQVAEWR
                     EQGLQTPHDFADLITLIRKGELVELPALGQDYILYGVGYSASDEPFTHYDKVAGKSVT
                     LYATDAELEQEQDQIADSLKLLEENVTSLKNELKAAPKRDRALRTRLQSEVVENERAV
                     KALRERKKLLDSFYRNDDARRLLAAEYETISTLARDFGGRAYDLQDATQRKALKVRLL
                     SHLRPAALKVLEEVAKTYRDKFGRPLAVTSVVRPDEYQRLLRETNANATLIEVPPHTT
                     GLAFDLYYRYMNAAEQTFIMSELARLQDQGRIEVLRENRDHYHVFAFADGRRPDEDLI
                     EQTLNGEATAQRPAQKKEVKEKVKEKSKEKVRERGAAAKSKGSKAAKKSRPRRA"
     gene            8528..9829
                     /locus_tag="VGB76_07715"
     CDS             8528..9829
                     /locus_tag="VGB76_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0098825.1"
                     /translation="MRLASMRRLKNLALILLLTGLPVYAQQSASKKFVFTTKSNEARA
                     AAEQAIKMIESFQPAPEILPLAQRAVAADPDFAFGHYLLATFTPPPQPGASPQPANTP
                     KAHMDKALELSKRASDGERRYIEAVALVRAQKPADALPLLKQLVADYPEERMARMMLG
                     QVLFNRGELQEARTHLERALALDNSTPRVYNLLGNYHLLQSDYASARAMYQQALKRTV
                     KGTAPFGPSFGLTYTHIYEGNIPEALKVLTRFQDQYARTSGASEFPPVFIWNAIARLH
                     LENGQPAEAIKFYERGYQTVPGSKLPAEEKMIWQGRLHHGRGRSLAKLGKHEEAWKEA
                     ELIKKMIDENPERGKQFMPSYHYIAGYLRLEGGDHAKAIEHLKQADQTDLFHKLLLAR
                     AYDRSGDTANAQKLYREIVESKQNNLERALAVPEAKKKLKG"
     gene            complement(10563..12065)
                     /locus_tag="VGB76_07720"
     CDS             complement(10563..12065)
                     /locus_tag="VGB76_07720"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0098826.1"
                     /translation="MSQSDNSSEYAANADGARVAIIGSGFGGLGLAIRLKQAGVEDLV
                     IFERAGDVGGTWRDNTYPGCACDVESHLYSFSFAPNPDWSRSFSPQPEIWEYLKRCAR
                     DFGLLPHIRFRHEVREAAWNEAAQHWRIETSQGTYTASVLVMASGALSEPDVPGIKGL
                     ENFRGKAFHSARWDHDYDLTGRRVAVVGTGASAVQFVPEIQPKVARLHLFQRTPPWIL
                     PRRDRALSARERRLFRRFPLTQRASRAAIYLFRELFLLPFRHFRLAGLVERIALRHLA
                     RSVPDETLRAKLVPAYAIGCKRILISNDYLPSLVRPNVEVVTEGIAEVREHSILDSSG
                     VERAVDAIILGTGFRVTDPPLAKHVRGRDGRTLSEVWAGSPQAHLGTAVTGFPNLFIL
                     LGPNTGLGHNSVVYMIEAQIEHVVAALGHAWRHGASAIEPRAEAQKRFVAEVQRRMEG
                     TVWTAGGCASWYLDSRGRNSTLWPDFSWRFRRRVARLNPDEYLLSFPPGA"
     gene            12614..12892
                     /locus_tag="VGB76_07725"
     CDS             12614..12892
                     /locus_tag="VGB76_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098827.1"
                     /translation="MNASGMTFVLSGVSAVLGMPFFQSIVADGSVRQDELDGTRENLS
                     AIVTTLRSTPPDKIEAMVAQYNEQLTAYLDKFNSEWGRINSTPSGTHN"
     gene            13132..13509
                     /locus_tag="VGB76_07730"
     CDS             13132..13509
                     /locus_tag="VGB76_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098828.1"
                     /translation="METQRTELLNQLPKHGWNVVSVEEAYLEWWADEMWLLESVWSPV
                     GSRAYVTFLVDPQAPAARKKGEYVWAAKASSGKPNQWLGEEGEFTLSLTAGWKEEMPA
                     FFEHLSGLRNQNETPASSPDAAP"
     gene            14163..>15799
                     /locus_tag="VGB76_07735"
     CDS             14163..>15799
                     /locus_tag="VGB76_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HEY0098829.1"
                     /translation="MEEKKRWTVMVYLAGDNNLSEECIYALKELKKVGTKKQDGTPEV
                     RVAGESDRQRLARRSNEPRNVNVIAQFDPSGRGNQTRRFHVGDEDVEGGIEDDVVKVL
                     REETDTGAGACLLDFLLFCIKNFPADYYMVVLAGHGTGTDQDFFLRDEGRPFSLIPSS
                     LSIPDLQGVFQDEQLTKALGKKRINILGFDACLMGMTEVCYQLRDSVVDLMIGSEGFS
                     PTAGWPYHRVLRVLNDCSSIAPNRFARKIVRRFTRFYRNYEFGGLSVDQSVLRVANIR
                     RLKDKVEELTDVLISKLKEDHETKREGSEKTEGRGYDFPRPRFIDAIIMSHWEAQSYN
                     GEQFVDLFDFCDILRRRYDSKFEVDEHEKYWADRVGAACEQVMCAIVGPNHPRLGESA
                     CENMSDGEAVVLKTCYNGPTFQFSYGLSIYFPWSATEFANHYFHDDDRQAVRAGQVAP
                     HSHLDFPIRSGWGKFLQLYFEATILRTPRQDDNRLNEDVRSGPPVSKGPTAPVHSMRN
                     PPRHYYPSECLKDKKTNQEERRPQQQQQQQQQQQQQQ"
BASE COUNT         3368 a         4543 c         4638 g         3250 t
ORIGIN      
        1 aattttctga cctctatttt gcccgccacg acgccccgct tgaatgagat tgggatcgac
       61 gtttgggtgc ttggctttac attcttcatc tcgttgctca cgggactcgt tttcggattg
      121 gctcccgcgc tcaaggcttc caagctgaat ctgattgaag ctctcaagga tagggtgcag
      181 ggcgcagcgg aaggacttcg ccgcaaccgt gttcgcagcc tgctggtggt cagcgagatc
      241 gcgatctctc taatcatact gatcggggca agcctgctgg tgaagagttt ctggcgttta
      301 caagaggttg acgcgggctt caatccggca aacctgctaa ccctacaggt ttcgcttccc
      361 gctgcaaaat atagcgaacc ggcgaaacag agagccgtct ttcaggagat atttcagcgt
      421 cttgaaagtt taccgggggc gcaggccgtg ggaatctcca atgattttcc gttggccggt
      481 aacatgcaaa ccagccagcc ctatatcgaa ggacgtgtca ccatcccggg gcaggaatcg
      541 ctctccggcg tccacgcggt cagccacaac tattttcagg cgatgggtat tcctctcctg
      601 aaaggtcgtg cgtttacggc cagcgacata gagagcgcgc agccggtgat gatcataaat
      661 aagatgatgg cgcagcgtct cttcgccaac gaagacccgc tcggtaaacg ggttaagttt
      721 tccgatgatc cgaatgtgcc gtggaatgag gtcgtcggag tggtcggcga tgtcagacac
      781 aatgggctgg atatagaacc tcagatggag acttacctgc catatccgca aagcccgaga
      841 acgataatgg caattgccgt tcgttccacg tccgacccga cagccatgac ctctgccgtg
      901 cgtcgtgcga ttcttgaagt tgataatgct caacctatct atgacattaa aactatggag
      961 cagcggctct ccgaagcggt cgccccacgc cgcctgtcaa tggctctgtt cagcttcttt
     1021 gcggcggtcg cgttagtgct ggcaatcgtc ggcgtctatg gcgtgatgtc atattcggtg
     1081 aatcaacgta cgcatgagtt cggcatccgc atggccttgg gcgcacagca taccgatgtg
     1141 cttaagctgg tacttaggca gggagtcatt ttagctatag cgggcatcct catcggagtt
     1201 atcgccgcct acgcggtaac ccgcttcatg tctggcttgc tttacggggt gagtgcaacc
     1261 gaccctctca tctttatagc cgtttcatta gtcttaatga taattgccct gctggcctgt
     1321 tatatcccgg cacgaagggc aaccaaggtc gatccgataa ttgccttacg ctatgaatga
     1381 actatgcgcc tgtgggtcat gttgccggaa tagaatttca gtgactctcc cacaggttct
     1441 ctcaacacgc cctaccggga ctactcatgt gcaaagcctc cgcctcgccc tttaagtgac
     1501 ggcgctagct gcccgaactt atcctcctgc gccttcggcg aaactgccgg tatcagcttt
     1561 ctgatcgaga catcgggaag ctgatacgtg cgcgtctgac cgttaagcca gcagcatcgt
     1621 taattcaggt gatatacagg tgccttgatt gcgctctgta tgtctctctg ctcctcacta
     1681 acctgtctgt taggataacc tcacgccaat cccaacaacc aactaaataa gaggtatgaa
     1741 aaatgcgcat agcattcgta cacacaccca tggcgacctt gccggttcct gaacggcagc
     1801 tcttttggcg caactttgat attcagtacc atgcggcgca tcccggcctc cgtcacatga
     1861 agaacaatct gtgggaactg cctcactgga tgcactggtt aggaggggtt ctagtccacg
     1921 aaggttttcc cctgcttgat gtcatcgatt tctatacctc cgaaaccgca ttcagcggaa
     1981 tcaaccggac gaaagtgcgg gaggttctct cggagcatcc ggcggatgtg ttcctgttct
     2041 ctccgatgac gccgaatctt cccttcgcct agggtgcagg tggatcgaga ttggactgga
     2101 gacgggtaat caggagagcc aagacctcta caagcaacgc gtgaagctcg accccatcga
     2161 agaaacattg atgaaactgc gcgacgaagg aatcgctacc tgctcgttca tggtcaatgg
     2221 ttttcctaat caaacgattg acgacatgaa gcggtcaatc gagtgggtct gtaccctgat
     2281 tgcaaaagac ctgctacagg cttcatacct cttcgggttg gttccttatc ccggcagcga
     2341 gatgttcgag catccggaga aattcggaat gactctgcat cataaaaatt tcaagctcta
     2401 tcacgaagag atgccgcccg tgttcgactc gccatacgct aagtctgatg ctgtctatga
     2461 acagtttctc agtggcgtaa agaatctggg tcaggctatg tcgaagaaag cttacttcgg
     2521 agaaatacct cctcccacag aactctcctt gtatggcact ttctggtcag accctcatgt
     2581 ctgaagacga agcaaatgct ttgataagcc ttaacctgca aaaagccctc tcgtcatctc
     2641 gtaactcttt acttcacgcc ggacgattag cgatagttct gcaagacttc cgttagccct
     2701 cgataccgaa caaccaggca acgaacctcg cctgcccata agcatcaacg cctgacaatc
     2761 tttcgacctc tcccttcctc ttcaccttcc ccggttaaat gccttcgagc gtccgttcga
     2821 gcagtgtgaa acctccggaa gcactgtgac gcccgcacct tcttttttaa tgaagcggta
     2881 gccctgcccg atcacattgt gctgatacct catgaaatgt ctcttattga gaagttgaaa
     2941 gcctttagaa gctgaatttc gtgccaaact ccatgcgcct gccgggcaaa gcggcggtcg
     3001 gcaggccgta gaagggcgag ctttgcacgc ccgtgaagtt gctgagattg gtgtggttga
     3061 acacgttgaa ggcttgcgcg taaaactcca tgcggaactt ctggttgctt gcgcccggca
     3121 gcgacgggac gccgccatcg cccatacgga tcatcaccgg tccagccgaa gcggacgctt
     3181 cgcgcacgcc gccaaacccg aaactccaac ccagacgcgt gctcacatcc caacgcccct
     3241 tgccgcgcgc gctgttacga ccgacgcccg ccgggcggtc gttgctcacg gtgtcgccgt
     3301 tatcgtcgaa gcccgtcgtg atgttgtagg gcagggcgga gttggcgttg aaggtcgcgc
     3361 cgagcgagaa gcctttgaag agtttgcggt tgaccatggc tgagaagaag tggcgcatgt
     3421 cgttcggggc ggggcctagc tcgccgcgca gattgaagtt gtcggcgggc aaactgagcg
     3481 cgccgctcgt gtcgctgatg ttgcgcgaat agacgtagaa ggttgaccag aagaggttgc
     3541 tcgtgaagat ggcgggggcg aaatgaactg aaaggcgatg cgaggttgaa ttcgcagacg
     3601 actcgatctg cgtgatgttt ccggcggtcg ggttggggcg accgaagccc tgcacgggcg
     3661 cgttcaggtt gcgcgcgcgc agcaggtgtt ggccgcgctg gaactggtaa tcggtgttca
     3721 ggcgaaagcg tttgaagagg cgcgactcga cggcgagcga gccttgcatg atcgtgggtt
     3781 gtttgagcag cgggtcaacc tgtatgcgac cgggcggaag gatgatcggc gtgcccgtgc
     3841 tgagcgggtc ggggtagccg ggattttgca cgacgagatc gcgctgcctg cgcccgtcaa
     3901 cgcgcaacgc ctgttcgtag gttccggcct cgaaccaatc gtagaagatc cccgccccgg
     3961 cgcgaatggt gaccgtgccg gatttgttcg gcgtccatgc gacgccgaag cgtggtgcga
     4021 agttgctgcg gtcggaaaga ttagtttgca gttcgtggcg cacgccgtag ctgagcagca
     4081 ggttcttgcg caggcggtag tcgtcctgcc agtaccatgc gaattgatat tgcgagaagt
     4141 tgagcgcagc ttcgcccgtg cgttgcgtgt aagtggtcgg gcgcgcggcg ttgaaggctt
     4201 cgaggttggc gaaggtgtaa gtgccgttcg ggttgacaga ctccgtgctg tcgtaactgc
     4261 cgccctcgaa aagcacgccc gcacgcatgg agtgttggcc gaaggagaga tcgaagttgt
     4321 cggccagttc aaaatcgagc gtttggtgtt gcccggcggt ttgcgcgccg ccgcgtgtga
     4381 aggcgttgag gacgagcagc gtgggcgcgt ttgagagggg aagcgtctcg cgcgtgcgcc
     4441 agcgcgcctg aaagcgaaac tcgttgagga agcgtttggc aaagacgccc gactcggaga
     4501 gacggaagat ttgttcggat tgatcgagcg tgtaggcgcg ttcggcgagg tcgaaatcgc
     4561 cgacgccgag gttgtcctgt cgtccggcgt tgcgctggta ttccagacgc agggagtgtg
     4621 tcttgtcgag cgcgtgttcg acgcgcgccg cgatgttgag acgacgcgaa ggccgctgga
     4681 tgagatcgtc gaagaagccg ccgggcagcg cggcgacgat tgttttcgag tcgtagttct
     4741 cggtgctgtc aacattgagg aagagggaag tgtggttgcg ccagagcggc ccatcgagtg
     4801 tgaagccgaa gcggcgcagt tgttccgcac cacgacgcga ggcgaaggcc gggcgcgcgc
     4861 tcagggcttc gtcgcggaag ccgaaactga gcgagccgtg ccagtcgttg acgccgggtt
     4921 tggtgaagat gtcgacggcg atgaagccgc gctcgtggtt ctccgcggcg taggcgttgc
     4981 ggcgaaaacg tatctcgcgg atttgcgatt tgggcgggag tttgccgccg cgaaagccgt
     5041 tgacgcgaat ggtcgcgccg ggtccggcca gcgcgttgag ggcgttctca aactcttcgg
     5101 ggtcgtcggg caaactcgcg atctgttccg ccgtcaggac gtttgagaag gcgtcgccgc
     5161 gcgggtcggt gctgcgctcc tgctcgtcct gcccgacgac gacctcttcc ttaaccgtcg
     5221 ccacctccaa ccgaacctgc gtctggttac cgcccgcgtt cagtgaaaac ccctccacga
     5281 tgcgtgtctc aaacccctcg gcggagactt cgagcaagac gcgcccggcg ggcaagccct
     5341 gaaagacgac tccgccccgc tcgtctgtct tgagtgcctg ctccgcgccc acccccatct
     5401 cgatacgcac gcgtgcggcg gcgacgaccg cgccgttctg atcaaccaca gagactttca
     5461 ggctcgcatc ccccgcgcct ctgctctgcg ccggaacgtt gacgaacatg aacgaaaaga
     5521 cgagccacag gctcagcatg cggatggata gacgatagcc acgcattgcg agaatctccc
     5581 tttttaatca agtgaactgt ttttgggttt aactatttct tctgaaattt gtcggctttg
     5641 aactgcgggt tgaactcgta ggtcttgatc tcccactcct cgttgacttc gccctccgag
     5701 ccttgcgtga tgcggtgcgg cagcatgagg ccgccggttt tgcgatagtc tgcgaagcgc
     5761 aactgcatct ccacctcttc gggtttcgcc ggagcgggtt cctgtgcggc cttctcgcgc
     5821 gcttccttga tgatctcctc cggcttcttc ttatcgccgc cgttgctgcg catcatggtg
     5881 gtgaacatgc gcggcttcgg cgcgcggtag gtgagcatca ggggcaggtg agtctctcta
     5941 tcgagaaaga gacgcgcggc gaaaccttcc gcgccggtcg cgtcaatcac gtctgcgcgc
     6001 ccgtcgtcgg caaccgcctc gcccgcgtag ttgaactcca gactgtaccc ggatggcgcg
     6061 ctgagcagga gcgcgaggac gtagcgcgcg tactccgcgc ggaacctttg cgcctgttcc
     6121 cgctccatct gttccaaatg ctccggcgaa ggtttctggc tgttcccgcc gatgataaac
     6181 atgccgcctc tgcccccgcc tccgccggtc cagtgttcgt ggccgttgag cgcgcgatag
     6241 gtggtgatgc tcgtgctcga accgccgacg atcatcgtgt cggatttcaa gaacttatcg
     6301 ggcaggagaa aattgaactc ccgttcaccg gccatctcgc gttcctgtat cacgcgccgg
     6361 aatttgccct tcaacgtcaa actctgaatg gagccgagct tctcttcgcc gccgatggcc
     6421 gcgcgcgcct gtttgagaat ctccagcgcg cgcgccgcat cgccgttacc tttggcgaga
     6481 gcattgaatg agcctgcggt gaacagtaag agcagacaag cgaagttgat gaatcgctgc
     6541 ataaaaagtt tctccttaag tcgaatagta gattgacggg cagccgggca cactccgtca
     6601 cagacgccga cacatactgc ggcgcacacg cgcgctatgg gcgagcaaaa tggtttccga
     6661 cgattcgccg gtttgaacta aggtgggctt gcgagccgtt ggccgctgac ggaagtttgt
     6721 taacccacgg acattcttat ttcaggcggg gcaggtgaaa agttccagaa agtgatctga
     6781 acttcgagag aacgttaaag gctgtatgat tgtagcggga tagatgggct attttgaacc
     6841 ggcgggcagg gcttgcccgc aaagagattt cgcgggcgcg ccgatccttt atgtcgcgca
     6901 tctgaagctt atgatttcgg gagctatcat tttcgtcaac gccgcacgtc agccgtcgcg
     6961 cgtcctgttg ctgttactgc tgctgctgac gctcgcgctg ctcgtaggtt gccgccggga
     7021 taggcgggac gccgtgatgc ccgtgcccgt gacagtgccg cccgccctgg ttgacccgtg
     7081 gcggaaagcc gcgcttgagg tcgaggagga tcgaggcgag ccggtgggac gacaggcgaa
     7141 ggtcgaagtt cccgcccagt tgaagcacta tgacaacccg cgacgtttcc tcgggattca
     7201 agtcgcggag tggcgcgagc aggggttgca aacgccgcac gactttgccg acctgatcac
     7261 gctcatcagg aagggcgagt tggtcgaact gcccgcgctc ggccaggact acatcctgta
     7321 tggcgtcggc tatagcgcct ccgatgaacc gttcacacac tatgacaagg ttgcgggcaa
     7381 gagcgtgacg ctctacgcca ccgacgccga acttgagcag gagcaagacc agatcgccga
     7441 ctcgctcaag ttgctcgaag agaatgtcac cagtttgaag aacgaattga aggctgcgcc
     7501 gaagcgcgac cgtgctctgc gcacgcggct ccaatcggaa gtcgtagaaa acgagcgcgc
     7561 cgtgaaggct ctcagggaga ggaagaagct gctcgattct ttttaccgga acgacgacgc
     7621 gcgccgcctg ctcgccgccg aatatgaaac catcagcacg cttgcgcgcg acttcggcgg
     7681 gcgcgcctat gacctgcaag acgccaccca gcgcaaagcc ctcaaggtgc ggctcctcag
     7741 ccatctccgc cccgccgctt tgaaagtgtt ggaggaggtc gccaaaacat acagggacaa
     7801 gttcgggcga cctctggccg taacttccgt cgtgcgcccg gacgaatacc agaggctctt
     7861 gcgggagaca aacgccaacg ccacgctcat cgaagtgccg ccgcacacta ccggactcgc
     7921 cttcgatctc tactaccgct acatgaatgc cgccgagcag actttcatca tgtccgaact
     7981 ggcgcgccta caagaccaag gccgcatcga agtgctcagg gagaaccgcg accactatca
     8041 cgtcttcgcc ttcgccgacg gacgccgccc cgacgaagac ctcatcgagc agacactcaa
     8101 cggcgaagcg acggcgcaac gacctgcgca gaaaaaagag gtcaaggaaa aggtcaagga
     8161 aaagagtaag gaaaaggtca gggagcgggg tgcagccgcg aagtcgaaag ggtcgaaggc
     8221 cgcgaagaaa agccgccccc gccgcgctta actttacgac cgcgcttatg cggcctccca
     8281 cacggcgaga tggaaaggcg cgcggcgtcg cccgcgccgt cgtggttgaa ggagacggac
     8341 gcgtccttcg gcaatcctct gtcatatgga gggcgtcgag ccgtgtgcga taaaatagtc
     8401 tcgaaagggc gcgccgcttg aaacgttcct gtgcccggtt acgttaaacc atcttggcta
     8461 tatggtcgct ttagcttcac gcaaaaatag gtaactttca aacgattaaa ttctcaagga
     8521 gaaagagatg agacttgcat ccatgcgtcg cctgaaaaat cttgccctca tactgctact
     8581 taccggcctg ccggtttacg cgcagcagtc agcaagtaaa aagtttgtct tcacaaccaa
     8641 atcgaacgaa gccagagcgg ctgccgagca ggccatcaag atgatcgagt ctttccagcc
     8701 cgccccggag attctgcccc tcgcgcaaag agccgtcgcc gccgaccccg acttcgcttt
     8761 cggacactac cttctcgcaa ccttcacgcc gccgccgcaa ccgggcgcgt cgccccaacc
     8821 ggccaacact ccgaaggcgc acatggacaa agccctcgaa ctgtcgaagc gagcgtctga
     8881 cggcgagcgt cgttatatcg aagcggtcgc gctcgtgcgc gcgcagaagc ccgccgacgc
     8941 gctccctctg ctcaagcaac tcgtcgcgga ttatccggaa gagcgaatgg cgcggatgat
     9001 gctcggtcag gtcttgttca atcgcggcga gctgcaagag gcgcgcacac atctggagcg
     9061 cgctctcgct ctcgacaaca gcacaccgcg cgtctacaac ctgctcggca actatcacct
     9121 gctccagagc gactacgcca gcgcgcgtgc gatgtaccag caggcgttga agcgaaccgt
     9181 gaaaggcaca gcccccttcg gcccgagctt cgggttgacc tacacccaca tctacgaggg
     9241 gaacatcccc gaagcgttga aagtcctgac gcgttttcag gatcagtatg cgcgcacgag
     9301 cggcgcgtcg gagttcccgc ccgtcttcat ctggaatgcc atcgcgcgcc tgcacctcga
     9361 aaacggccag cccgccgagg cgatcaagtt ttacgaacgc ggctaccaga ccgtgccggg
     9421 gagtaaactg cccgccgagg agaagatgat ctggcaggga cgcctgcacc acgggcgcgg
     9481 acgctcgctc gccaaactcg gaaagcacga agaggcctgg aaggaggccg aactgatcaa
     9541 gaagatgatt gacgagaacc ctgaacgcgg caagcagttc atgccctcgt accactacat
     9601 cgcgggctac ctgaggctcg aaggcggcga ccacgcgaaa gccatcgaac acttgaagca
     9661 ggccgatcaa accgacctct ttcacaagtt gctgctggca cgtgcctacg accgttcagg
     9721 cgacacggcg aatgcgcaga aactttatcg tgagatcgtc gagtcgaaac agaacaacct
     9781 cgaacgcgca ctcgccgtcc cggaggcgaa gaagaagctg aaaggctgag agccgcacgg
     9841 tcagccgttg agtggaagcg aacagcagga gcacggcctc tattcaggag tccgtgctcc
     9901 tgcatagttt cggaaatcgg ctgtacgcac gtggcttgaa taaatcttcc gctgaaagga
     9961 ctgcctgtaa cctgtcagaa gctgctacat aggttcctct caacaccggc atcagaagcc
    10021 tgcatctaaa gccctgcatc gcccgccgcg cccggacatg agcgttgaca tcgtataggc
    10081 gcaactgtac tctcgatatg tggcctcggc gaagcgagcc atccccggtc atcctcatcg
    10141 gcaaaaactt ttcacacgcg cctttggata ggcgataaaa atagctcccg accaatttcc
    10201 gagttcggtc gtgcgcctgc gtcgtgcgcc tctgcgtcgt tacgtcgtcg ccgcgccgga
    10261 ctttgccggg ttgattcgag tgtgttgcgg ctgatcggaa ttttgtcttg aaggtaacag
    10321 atgatgagcc aagagttgac caactgccat gaggtgctgg gggtggccgg gggagcttcg
    10381 gcgcaggaac taaaagtagc gtatcgcgat ctggctaagg tctggcaccc cgacagattc
    10441 gctcatgacc cacgactgca acagaaggca caagagaagt tgaaagagat caacgaagcc
    10501 tacgaacagt tgacctccgg caagatcaga ttccagaagc gaacgtcaca agaggcgcaa
    10561 actcacgcgc cgggtggaaa actgagcaga tactcgtccg ggttgaggcg cgcgacgcgg
    10621 cgacgaaacc gccaactgaa gtccggccag agcgttgagt tgcgccctct ggaatcgaga
    10681 taccaactcg cgcagcctcc cgccgtccac accgtccctt ccattctcct ctgtacttcc
    10741 gcgacgaacc tcttttgtgc ttctgcgcgc ggctcgatgg cgctcgcacc gtgtcgccac
    10801 gcgtggccga gtgcggcgac cacgtgctcg atctgcgctt cgatcatgta gacgaccgag
    10861 ttgtgtccga gtcccgtgtt tggtccaagc aggatgaaga ggttggggaa ccccgtcacc
    10921 gccgtcccca gatgcgcctg cggactccca gcccacacct cggacagtgt gcgcccgtcg
    10981 cgcccgcgca cgtgtttagc gagcggcggg tctgtcacac gaaagccagt gccgagtatg
    11041 atcgcgtcaa ccgcacgctc cactccgctg gagtccagaa tcgaatgctc cctcacttcg
    11101 gcaatccctt ccgtcaccac ctcgacgttc gggcgcacga gcgacggcag gtagtcgttc
    11161 gagatgagga tgcgcttgca accgatggca taggcgggca cgagcttggc gcgcagcgtc
    11221 tcgtccggca cggagcgtgc gaggtgtcgc agcgcgattc gttcgacgag tcctgccaga
    11281 cggaagtgac ggaagggcag caggaagagt tcgcggaaga gatagattgc ggcgcgtgag
    11341 gcacgctgcg ttagtgggaa gcggcgaaac aggcgacgct cccgtgcgct gagggcacgg
    11401 tcgcgcctcg gtaatatcca cggcggcgtg cgctggaaca agtgtagccg ggcgaccttc
    11461 ggctgtatct ccgggacgaa ctggacggcg gatgcgccgg tgccgacgac ggctacgcgc
    11521 cgtcctgtca gatcgtaatc gtgatcccac cgcgcggagt gaaacgcttt gccacggaag
    11581 ttttccaacc ccttgatccc cggcacgtcc ggctcgctga gcgcacccga agccatcacg
    11641 agcacggagg ccgtgtaggt tccctgcgag gtttcgatgc gccagtgctg cgcggcctca
    11701 ttccaggcgg cttcacgaac ctcgtgtcgg aagcgaatgt gcggcagcag tccgaagtcg
    11761 cgcgcgcagc gtttgagata ctcccagatt tcgggctgcg gcgagaaact gcgcgaccag
    11821 tccgggttcg gcgcgaagga gaacgagtag aggtgtgact cgacatcgca ggcgcagccc
    11881 ggataagtgt tatcgcgcca cgtcccgccg acatcgcccg cccgctcaaa aataaccaag
    11941 tcctcgacgc ccgcctgctt gagacgtatg gcgagtccca gaccgccgaa gccgctgccg
    12001 atgatcgcca cccgcgctcc gtcggcgttc gccgcatact ccgaactgtt atcgctttga
    12061 gacacagcaa gcatccttca agtatttgct gcgagcgtct ttcaaatgtt tgccgcaagc
    12121 gttcgttgat tgaatgactt tcgcctcaag tgcggattct gttaagcgag cttcgacgag
    12181 ggtagaaact cccaccggcc aaccaacgtc gttttgagta tcgcccgcaa gaccggaaca
    12241 ggaaagttta tccacgtcaa cgcgatgatg atacgacgat aggtgctctt tctgtccacc
    12301 cgaatctcca agtacccgct gccttcctta cagggttcat tatctctgcc cgtgtgaaaa
    12361 atgtccggcg acagcagaat ggagttgctg cactgtttca cgttaggcgg aataataagt
    12421 aaggttatat tgatagaacg ggtcaagatg gttatccccc tctcgattcc ggtgtgaggc
    12481 tacagcctac tctctccaac aagcatttga catccggagg cagcatcaat attgtgctgc
    12541 ggttccaccc gctgatggaa cctgctttat gcgtcggctt catcttcaat atctctcaga
    12601 gggaaaatac gacatgaacg cttcgggaat gacttttgtg ctgagcgggg tttcggctgt
    12661 tttggggatg cccttttttc aatccatcgt agccgatggc agcgtcaggc aagatgaact
    12721 tgacggcaca cgcgaaaatc tttcggcaat tgttacgact ttaaggagca cgccgccgga
    12781 taaaatagag gcgatggtcg ctcagtacaa cgagcagctc acagcctatc ttgataagtt
    12841 caattccgag tggggcagaa tcaactcaac gccttcaggg acgcacaact gatggccggc
    12901 tacgttacga cgatcatggc taagtccgga ctcaaagatt agctcgcgct gagcagtggc
    12961 ttattctctt tcgcgcgtaa gcccctgtta tccccgcccg ggcataacct tcgcagtggc
    13021 cgccctcact acccattcag acaggttcgc aattcggtag tctcttaaat tggatgttga
    13081 taactcgcct ctcaattcag gtgtggggcg acgaacattg taggcagttg tatggaaact
    13141 caaagaacag aattgctcaa ccagcttccg aagcacggtt ggaacgtggt tagtgtagaa
    13201 gaagcttatc ttgagtggtg ggctgacgag atgtggctgc tggagtccgt gtggtcgccc
    13261 gtcgggagtc gcgcttacgt cacgtttcta gtcgatccgc aagcccctgc tgcgcgaaag
    13321 aaaggtgagt atgtctgggc ggcgaaggct tcttccggca agccgaacca atggctcggg
    13381 gaagaaggtg aatttacgtt gagcttaacg gcaggctgga aggaggagat gcccgctttc
    13441 ttcgagcatc tgtccgggct gcgcaatcag aacgaaacgc cagcttcctc gcccgacgcc
    13501 gccccataat agtctgcact taagcgcgaa tagcgttccc cgtcattcga caattctcaa
    13561 gtcagatgaa tagcacccgg catgtcttga gaggtgccgt gcgtcgccgt cttataaggc
    13621 gtgtgcgatt cacacgctcc cgcagaagtg tttctcggaa atgttttccg gactttatac
    13681 atatcacgat gaaggcgcat ttttagggat taagggagag gtgagcgatg gcaggtgagg
    13741 gcgaggtgct gcccgtcgtt cacccggcgc gggtgttgac cgagaggccg gtaaagtgtg
    13801 cttcgccgga aactctttcg cgtctcccgt ctccaaccgg aaagcattgt gatggaaatg
    13861 gtcagccgcg tgacccggag cgataagccg gacagtggtt ctgcatttca taggattccc
    13921 cgactacatt cttaagttcc ccgtctcgcc gcccgcaaag ttgtgaggtc gcccggcctg
    13981 accgtatccg ggaagactgt tgactgaagg caggcgcgag gcggctcaca cgccctgccg
    14041 cgctctggac ttaaaggctc ataaactctg cgcctctcgg tcgtcagccc gtgcgtccgg
    14101 gtcgtgctgc ggtttccggc gagcattttc ttgaacgcga cgatggcaaa ggagctacca
    14161 ccatggaaga gaagaagaga tggactgtga tggtttacct ggcgggcgat aacaacctga
    14221 gcgaagagtg catctacgcg ctgaaggagc taaagaaggt cggcaccaag aagcaggatg
    14281 ggacgccgga agttagggtg gcgggcgaat cggacaggca aaggctcgcg cgacgttcca
    14341 acgagcctcg caacgttaac gtcatcgcgc agttcgaccc gagcgggcgc ggtaaccaga
    14401 cacgtcgctt ccacgtgggc gacgaagacg ttgaaggggg catagaggat gatgtcgtga
    14461 aagtgctccg ggaggaaacc gatacgggcg cgggcgcgtg cctgctcgac ttcctgctct
    14521 tctgcatcaa gaattttccc gcagactatt acatggtggt gctggccgga cacggcaccg
    14581 gcacggatca ggactttttc ctacgcgacg aaggccgccc cttctcgctc atcccctcat
    14641 cgctcagcat tcccgatttg cagggcgtct ttcaggacga gcaactgacg aaggctctcg
    14701 gcaaaaagcg catcaacatt ctcggcttcg atgcctgcct gatgggtatg acggaggtgt
    14761 gctaccaact gcgcgactcc gtggttgacc tgatgatcgg ctccgaaggg ttcagcccca
    14821 ccgccggatg gccgtatcac cgcgttctgc gagttctcaa cgactgctca agcatcgcgc
    14881 ccaaccggtt cgcacgcaaa atcgtgaggc gcttcacgcg cttctaccgc aactacgagt
    14941 tcggcgggct gtccgtagat caatcggtgc tgcgggtggc taatattcgg aggctgaaag
    15001 acaaggtgga agagttgacg gatgttctca tcagtaaatt aaaggaagac cacgagacta
    15061 agagggaagg atcggagaag actgagggta gaggttacga ctttccccgc ccgcgcttca
    15121 tagacgccat catcatgtct cactgggagg cacagtcgta caacggggaa cagttcgtag
    15181 acctcttcga cttctgcgac atcctgcgac gacgctatga cagtaagttt gaggttgatg
    15241 agcatgagaa gtattgggct gacagggtgg gcgcagcttg cgagcaggtg atgtgcgcca
    15301 ttgtgggtcc aaatcatcca aggctggggg agtcggcctg cgaaaatatg agcgacggcg
    15361 aggccgtcgt attgaagaca tgctacaacg gccccacctt ccagttctcc tacggcctct
    15421 cgatctattt tccgtggtcg gctaccgagt ttgccaacca ctactttcat gatgacgacc
    15481 ggcaggctgt acgggcgggg caggtggctc cgcatagcca tctggacttc cccatacgct
    15541 caggctgggg taagttcctc caactatatt ttgaagccac gattcttcgc accccccgtc
    15601 aagacgataa tcgcctcaat gaggacgtac gttcagggcc gcccgtcagc aagggaccaa
    15661 ccgctcccgt gcacagcatg aggaacccgc cgcgccatta ctacccgagt gaatgcctca
    15721 aggataaaaa aaccaatcag gaggaacgaa ggccgcagca acagcaacaa cagcaacaac
    15781 agcaacaaca gcaacaaca
//
LOCUS       DASWNS010000225         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_319739, whole genome shotgun sequence.
ACCESSION   DASWNS010000225 DASWNS010000000
VERSION     DASWNS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319739"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..530
                     /locus_tag="VGB76_07740"
     CDS             243..530
                     /locus_tag="VGB76_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098830.1"
                     /translation="MKRNLAYTAATLALAVTAMLAPFNIAPVKAGQGQTTQACEDCKA
                     AAQGRLSACDFTARNDNDRRTTRGQCVKQFEAEVRECPCPPGHNNPNSTPR"
     gene            635..>1675
                     /locus_tag="VGB76_07745"
     CDS             635..>1675
                     /locus_tag="VGB76_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098831.1"
                     /translation="MRLPLFPHTLEPRRIERVCWLVAAALGFLQAWGRRHNSNDGLAY
                     MGADGISYLDIGDAYWRGDWQAAINAMWSPFYSWLTGLALRLIQPTPYWEFTVVQLLN
                     FSIYLFALFAFALFLRELERFRDSRTKEAAHFDALTSDADAQAIDGDAQNGSHTLPAW
                     AWLAFAYALFVWTSLSMNRVARTSPDVLVSALVFNASALLLRIRMRRAGWLTFALFGL
                     TLGVGYLSKTFMFPLAFVFLAVSLFAGGNVRRAVPRVALALFVFLAVSAPFVVALSRA
                     KHRLTIGDTARLNYAWHVNRTTPFIHWQGDAAGATGTPTHPTRKLFDSPAVYEFAAPV
                     SGTYPPWYDPTYW"
BASE COUNT          277 a          579 c          474 g          345 t
ORIGIN      
        1 agacgcgact cacgttccca cgcgaccgtc gcaggcgaaa gcagacgcgg ctggctcaac
       61 cgtattctta taatttagtt gcattttccc attggctttg acttgcgcgg cgcgtgtggg
      121 ttagcttgtc aacgttccca tttaaacccc ttttgaatat tggcagagtg tcggcggcgc
      181 gtcgtttcgc ggcgcgcggg cggctctctt tttaccacga acaacaggga ggatattgac
      241 tcatgaaacg aaacctcgcg tacacggcgg ccacgctggc tctcgccgtg acggctatgc
      301 tcgcgccgtt caacatcgct ccggtgaaag ccggtcaggg gcagactacg caggcgtgtg
      361 aagactgcaa ggccgcggct caaggccgcc tgtcggcctg cgacttcacg gcgagaaacg
      421 acaacgaccg tcgcaccacg cgcggtcagt gtgtcaaaca gttcgaggcg gaagtcaggg
      481 agtgtccctg cccgccggga cacaacaacc ctaacagcac accgcgctaa gttgcccggc
      541 aggatcaacg attgagaggg tgatgttcga gagatcgaac gtcgccctct ttattttagg
      601 cgcgggcggg cgttagactg tcggcgactt gaatatgaga ctcccgctat tcccccacac
      661 gctcgaaccg cgacgcatcg aaagagtctg ctggctggtt gccgccgcgc tcggcttttt
      721 gcaggcgtgg ggcaggcgtc acaacagcaa cgacgggctg gcctacatgg gcgcggacgg
      781 catctcctat ctcgacatcg gcgacgctta ctggcgcggc gactggcagg ccgccatcaa
      841 cgccatgtgg agtcccttct attcatggct gacgggtctg gcgcttcgtc tcatccagcc
      901 cacgccctac tgggagttca ccgtcgtcca actgctgaac ttctcgatct acctcttcgc
      961 gctcttcgcc ttcgccctct tcctgcgcga actcgaacgc ttccgcgata gccggacgaa
     1021 agaggccgca cacttcgacg ctctcacgtc agacgccgat gctcaagcaa tagacggcga
     1081 cgctcaaaac ggctcgcaca cgttgcccgc gtgggcttgg ctcgcgtttg cttacgcgct
     1141 cttcgtctgg acttcgctct cgatgaaccg cgtggcgcgc acgagtcctg acgtgctcgt
     1201 ctccgcgtta gtcttcaacg cctccgccct cttgctgcgt atccgcatgc gccgcgccgg
     1261 atggctcacc ttcgctctct tcggcctgac gctcggcgtc ggttacctgt cgaagacgtt
     1321 catgttcccg ctcgccttcg tctttctcgc cgtgagcctg ttcgctggcg gcaacgtgcg
     1381 ccgcgccgtg ccgcgcgtcg cgctcgcgct cttcgtcttc ctcgccgtgt ccgcgccttt
     1441 cgtcgtcgcc ctctcgcgcg ccaagcatcg cctcacgatt ggcgacacgg cacggctcaa
     1501 ctacgcctgg cacgtcaacc gcaccacgcc ctttatccac tggcagggcg acgctgcggg
     1561 cgcgaccggc acgcccacgc accccacgcg caaactcttc gattcgcccg ccgtctacga
     1621 attcgccgcg cccgtctccg gcacgtaccc gccgtggtac gacccgactt actgg
//
LOCUS       DASWNS010000226        18988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_138347, whole genome shotgun sequence.
ACCESSION   DASWNS010000226 DASWNS010000000
VERSION     DASWNS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18988
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138347"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2479)
                     /locus_tag="VGB76_07750"
     CDS             complement(<1..2479)
                     /locus_tag="VGB76_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEY0098832.1"
                     /translation="MKNIPNDPARSASGGQLTEPIAIIGMACRYPGANSPAELWQLLR
                     EGVDAVTEIPANRWDVDRFYDEDATVPGKMNTRWGGFLEQIDHFDAHFFRVSPREAIH
                     LDPQQRLLMEVTWEALEDAGQVPGKLAGSPTGVFVGIMGSDYADTPMNDPLLVNFYTV
                     TGHARCIASNRISHFFDFRGPSMSIDTACSSSLVATHLACQSLWRGESTLAIAGGANL
                     ILSPEGTIWYAKAGLLARDGRCKTFDSRADGLVRSEGAGVVVLKLLSRALADGDPIHA
                     VIKGGAVNQDGRSNGLTAPNRWAQEALIREACVQAGVSPAHISYVEAHGTGTSLGDPI
                     EAQALGAVLSTDRPEGDFCAVGSIKSNIGHLEAAAGVAGLMKVVLMLKHREIPPSLHY
                     QNPNPHIPFAELPLRVQQSLAPWPEGISPALVGVNAFGFGGTNAHLIVSEAPPVSEAP
                     HVGADPETERALSVETQTSTDAAYLLPLSARAPDALRALAEAYRRFLLDEANTASLRD
                     ICHTAAVRRTHHEHRLTLVGTSREDFAERLESFLANTPHPAISTGHVTHNRRASLAFV
                     FAGQGPQWWAMGRQLWRDEKVFRQTLERCDELFARVAGWSLLDELLAETEQQSRMADT
                     EVAQPCLFALQVGLAALWQSWGIKPEAVVGHSVGEVAAAHVAGALSLEDAVRVVFHRS
                     RLMQRATGGGKMAAVALHPVEVAPFLADYQNRLTVAAHNAPRSCVLSGESAALVDIVK
                     ALRARGLQAQELRGDYAFHSPQMKEFGDELEASLVDLEARRALIPIFSTLSGSACVGT
                     EFDAGYWGRQLQEAVLFAPVVDAMLAGG"
     gene            complement(2534..4327)
                     /locus_tag="VGB76_07755"
     CDS             complement(2534..4327)
                     /locus_tag="VGB76_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0098833.1"
                     /translation="MHIPEQYGMAEELEGYLGDPQDGRNGFSFAQAVALDEGEEYPEQ
                     ACALLDAWGLPDFYVPVQWGGRLRSYEELISLLRVVARRNLSVIVAHAKSFLGTVNVW
                     TGGSPEQRKWLAGIVMEGKQVALAYHEKAHGGDVLATDVKAVRVAGGYRLSGEKWLVN
                     NATRGVGLTVFARTAESGGPRGFSLFLVDKRALDPSSFEHLPRIKTLGVRGVDFSGIC
                     FRESFVSEDSLIGREGSALEQTLKAFQLTRIIIPALALGAADTALRSTLDFARTRALY
                     GTRVAALPQARRTLVEAFLDLLIGDCLTTACARAMHQTPEQMRLYSAVVKYYVPQSLE
                     ETIERLGVVLGARHYLREGHWHGIFQKLLRDNKVLSIFHTGTLLNLTTIGVHLRDLAQ
                     HRARTAPQADTDAAELSMRLASIFDLRHPLADFNPDGLSLYTRGRDDILNGLDLALSQ
                     LRLLSPHTPDADPLLQCLIAQTGRVLAQRQAADDEHRALAAQLGNNYSRAPEMFEQAE
                     RYCLLEAAACCVHLWLHNRETLGEFFGRGDWLVLSLERLLVRLGERRRVLPAEHEERV
                     AEELERLYEEERLFSVVPLQLARSQSVNRDA"
     gene            complement(4382..4699)
                     /locus_tag="VGB76_07760"
     CDS             complement(4382..4699)
                     /locus_tag="VGB76_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0098834.1"
                     /translation="MGTVDGDGRSAGAAGAQKGASLEEIQSWLISRLAELMRVSPEEV
                     DVHEPFANFGLNSIDAVSLSGDLEDFLRRPLPATLLWDFPTIEILSRHLSAEAAGDAA
                     ANR"
     gene            complement(4779..6557)
                     /locus_tag="VGB76_07765"
     CDS             complement(4779..6557)
                     /locus_tag="VGB76_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0098835.1"
                     /translation="MHIPEQYGAAEELERYLGDPQDGRNGFSFAQAVALDEGEEYPEQ
                     ACALLDAWGLPDFYVPVQWGGRLRSYEELISLLRVVARRDFTVGFAYSILSLLGSAAT
                     WAGGTDRQKQKVAQLLKGRQQIILAYNEKAHGGDFLATDVKAVRVAGGYQLSGEKWLI
                     GNAVRCVALTVIARTRDEAGPRSLSLFLVDKRALDPSSFEHLPRIKTLGVRGAHVSGI
                     RFKESFVDDDSLIGREGSALEQTLKAFQLTRIIIPALALGAADTALRSTLDFARTRAL
                     YGTRVAALPQARRTLVEAFLDLLIGDCLTTACARAMHQTPEQMRLYSAVVKYYVPQSL
                     EETIERLGVVLGARHYLREGHWHGIFQKLLRDNVVVRYHFNAALNLTTIGVHLRDLAQ
                     HRAHTASQTNDAISAELPARLASIFDLQHPLADFNPDGLSLYTRGRDDILNGLDLALS
                     QLRLLSPHTPDADPLLQCLIAQTGRVLAQRQAADDEHRALAAQLGNNYSRAPEMFEQA
                     ERYCLLEAAACCVHLWLHNRETLGEFFERGDWLVLSLERLLVRLGERRRVLPAEHEER
                     VAEELERLYEDERLFSVVPLQLASHN"
     gene            complement(6765..8531)
                     /locus_tag="VGB76_07770"
     CDS             complement(6765..8531)
                     /locus_tag="VGB76_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0098836.1"
                     /translation="MMQTQTFPQHRAAETLERLFGDPFAPENAFSFKNAVALDEREEY
                     PAQACQLLDALKVSDYYIPSTLGGKLASFEEFINVLRVIARRDLTVAIAHGKTYLGAI
                     ATWVGGSPEQQQRLAEIIRSDAQVALALTERSHGSDLLASEVEAGSIAGGYKLSGEKW
                     LINNATRARTLTVFARTGTASDPRGFSLFLLEKSDGARSTYRTLPKIKTHGIRGADIS
                     GIRFENTFLPADSVIGQVGAGLEIMLKAFQLTRTVITGLSLGAADTALRVALEFALAR
                     KLYGDSVFAIPHARRLLCDAFIDLLICECLTVASARSIHVEPEQMSLRSSISKYLVPT
                     MVEKLLKSLSIVLGARQYLRQEHCEGIFQKVYRDNAILSLFDGSTVVNLHAIAIQLGH
                     LTRGSQSTQEREAELSNQLARIFSLTEPLPDFDPAALTLHNRGRDSILQGFALSLEKL
                     SSIEPKETGDGATLALIESRARAILSEVESQREFITASLRDSGFNKSPEMFEQAERYC
                     LLEAAACCVHLWVYNRETLGGFFGRGDWLVLSLERLLVRLGERRRVLPAEHEERVAEE
                     MQRLYEEERLFSLVPFQLAPRN"
     gene            complement(8554..10338)
                     /locus_tag="VGB76_07775"
     CDS             complement(8554..10338)
                     /locus_tag="VGB76_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEY0098837.1"
                     /translation="MKLQPSTIDGFLRLRASDQPARTAYVYLGDGEQEQARVTYSGLD
                     LLARGIGATLCSLQARGERALLLYPPGLDFVAAFFGCLYSGVVAVPAYPPHPARPEIT
                     LPRLHSILADSQAKFVLTTTGILAKVAPLFARTEDFKHLRWLATDEIEHGRAEDWNEP
                     EPDADGLAFLQYTSGSTNAPKGVMVSHRNLLENSAYIHYGFEHTPESISVTWLPAYHD
                     MGLIDGIMQPLYAGFTCYLMPSVAFLQRPLRWLQAISRYRATHSGGPNFAYEHCVNKI
                     RPEQRGGLDLSCWRVAYNGAEPIREETLRRFAASFEECGFRWDAFYPAYGLAEATLKV
                     SGGKGAKPPVTCKLEAAPLEENLLVEASTAAPGAQNVRTLVGSGDVSLGTRAVIVEPA
                     SLRECPKGSVGEVWVSGAGVAQGYWNRPELTEQTFKAHLSDTGQGPFLRTGDLGFIKD
                     GELFITGRLKDLIIIGGRNHYPQDIELTVEQCHPSLRSGCVAAFSVEFEGEESLVIAA
                     EVDQRYKRTAPTGETAGAASRHFAAEEWKLLKTGVQRAIADYFGLHVHELVWLKVGGI
                     PKTSSGKIRRQACRQAYLTGRLERLPFN"
     gene            complement(10377..12812)
                     /locus_tag="VGB76_07780"
     CDS             complement(10377..12812)
                     /locus_tag="VGB76_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0098838.1"
                     /translation="MKNLLQDIRYGLRTMFKTPGLVLVVALSLGLGSGLNITIFSLIN
                     AILLRPLPGVAEEKQLFEVYTSSPGLRFGAVSYPDYVDYRDGNQVFSGMIAQRVTPVS
                     LSNGGNNEIVASAIVSGNYFNTLDVKPVIGRGFSAEEDQTPNSHPVVVISHSLWRRAF
                     GSAPDIVGKTIIVNSQSFNIIGVAPEGFIGTIIGLAPDIWFPLMMQKRVLPGSDRLTD
                     RGARWLNVLGRVKPGVSREQAEAGLQGIYNRLAQEHPGTNKATTPTVVPLGEGSTGIK
                     KNMSGVLWLLMAVVFLVLLIACFNVANLMLARALARHKEIGIRQALGATRTRLMQQLL
                     TESVLFSLLGGLVGLLIAFISTKLLLNLVPPTSIPVSFDLSLNRQVYIFALLVSVLTG
                     IIFGLAPAIQTTKTDLIPALKDEGSGQGYKKSRLRNLLVVGQVAISLILLICAGLFLR
                     SLQNAQNSNPGFEPEKLAIVSTDVGLQGYDRENGQRYYQQLMERVSQLPGVETASLAY
                     VVPLSGGGMQQMAVKIPGYEPPPNTQLAVDFNIVGRNFFQVMSVPFVQGRDFTDFDTK
                     TAPGVCIINEAMARRYWPDKNPIGQQFSLVGGKAPIEIVGLVKNTKYYNLKEEPIPYF
                     YLPFQQSYSARMTVFAKSNGAMGTLLPALRQEVATLDKTIPARIITMKDALGTVLIQQ
                     KLAAVLLSIFSLLALLLATIGLYAVMAYSVSNRTREIGIRMALGAQRGDVLKLVLKQG
                     MLLTLIGLGVGLVVAFAATRFLAGLLYGVSATDLTTFAAITLFQVVIALLATYIPARR
                     ATKIDPLRALKYE"
     gene            complement(12907..13704)
                     /locus_tag="VGB76_07785"
     CDS             complement(12907..13704)
                     /locus_tag="VGB76_07785"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEY0098839.1"
                     /translation="MLSALTRGVSPSIGDCKLTFMHREQVDVAAATRQHEAYERCLQQ
                     MGVRVTPLPLMPEAPDAVFIQDTAVVVDEVAIMATMGAACRSGEVESVTGALSSHRPL
                     RYLSPPATLEGGDVVRVGRTLYVGDSGRTNREGIRQLSEILAPYDYQVSPVGVKGCLH
                     LSTGCSYLGRDLMLVNSAWIDSSAFRYFKLLEVPDTEPWAANTIAVGDVVLVPSPFER
                     TRALIEAQGFQVITTDISELQKAEGALTCMSLMFNSQPSSDASQPAL"
     gene            complement(13736..15397)
                     /locus_tag="VGB76_07790"
     CDS             complement(13736..15397)
                     /locus_tag="VGB76_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HEY0098840.1"
                     /translation="MKIISFIFRYSKRTFILAAFAGAIAGISSAGLLALLMVALRDEP
                     PASLTLAWYFAGLLVVSVLANLFSRFLLIKISQQVPYELRLRLSRQAFNVPLRKFEEI
                     GSHRILAILTQDMSTIAIALLDFPVFCVNLAMAVGCLIYLCWLSPIVFAAFTFILIVT
                     MTSLQLVNRAAGRHLKRARDDWDVLLNHIRSLLDGTKELRLNRQRREAFFSELMMPRA
                     DSFRRNNSIGRQMLGVNAAGSQALYFIFIGLLVYGLPRLWPISSDTLIAYALAALYLR
                     TPMTIVLDIIPSFNNANISLTKVEETGLSFAGTDAVSEAAVDPEPTCQQLELSEVTHV
                     YKHESGDGDFLLGPLSMTLRQGELVFLVGGNGSGKTTLAKLITGLYIPESGEIRFDGT
                     PVTEQNRESYRQLFSIVFSTPHLFERLMGHNRLTVDQQAGDYLSKLQLDHKVKVKDGV
                     LSTTDLSQGQRKRLALLTAYLDDRPVYIFDEWAADQDPLFKQFFYLHLLPELKARGKM
                     ILVISHDDRYFSMADRIIKLEQGKIVEDALLGVSAYAPSTLPASL"
     gene            complement(15504..18968)
                     /locus_tag="VGB76_07795"
     CDS             complement(15504..18968)
                     /locus_tag="VGB76_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0098841.1"
                     /translation="MELPTERARPAVLSSQSAQQEVRLSEATVARLRELGQQEGCTLF
                     MSLLAVFQVVLWRYSGQPDIVVGTPVANRRHHGTEELLGFFVNTLALRTELRGELTFR
                     ELLGRVREVCLGAYAHQDVPFEKLVEELDVTRELNRHPLFEVMLVLQAGGGDAGLQLE
                     GLEVSAEGAGGGVLGAKFDVLLGLTEERGGGVRGKLEYRAELYSEALMQQLVRHLERV
                     VKEVLAERGEGKLGDLKMLSDEERQQQLVEWNTTARPYELERCLHHLFEAQVERSPDA
                     VALICGGQQLSYRELNRRANLLALHLRTLGVTSDSIVALLFDRSVEMVVSLLGVLKAG
                     GAYLPLDLAYPAQRLRYMFEDAAPRVLLTNEELLGKWADAPQLETATVVCVEQLGLGV
                     VTEQTKECPNLEVSIEADNLAYVIYTSGSTGRPKGVAVTHRSLLNFLHSMLREPGLSA
                     ADTLLAVTSLSFDIAALELYLPLVCGARLALASREEASDALRLQQRLAQTQATVMQAT
                     PATWRMLLDTGWQHDGTLTLLCGGEALPRDLSERLLESGAPVWNLYGPTETTIWSTLK
                     RISPADRAVTIGRPIDNTRVYVLDEQFQPVALNVTGDLYIGGEGLARGYLHRAELTAE
                     RFIADPHGAEPGARMYRTGDVARWRACGELEFLGRSDQQVKVRGYRIELGEVEAAVCA
                     VEGVRQCVAAVREEESGEKRLVAYVVGVEGERAPGWAELRVALRERLPEYMMPSAVVV
                     LEELPLTPNGKVDRRALPDPDGSRPELESRYEKPRTGLERFLVEMWEDVLGVETVGVY
                     DNFFDLGGTSIRVVVFVNRLQQRLGVPVSVKDLFGAPNVAALAAHLKEHYGRTVSAVC
                     GDDDGDASSLLNALQPVAGSWSPLVELQRGTGKTPLFFVHPVGGNVFCYFNLARRLGD
                     DQPFYALQSRGLSDEQHGHTRIEEMASYYIEHLRAIQPTGPYRIGGWSLGGVVAFEMA
                     RQLKAGGDEVVMLALIDSLSPSALAGMEPEDELSQLANFALDLGFTKAHLSRSRDAVR
                     SLPLEGQLAYMLELASAEGLVPAGLELSHLHRLFSVFKTNREAMQGYVPGVYAGRVTY
                     FQATGDGTNGAGDMSQGWHELTHGGVEVYRLPGDHYSILKQPYVDALAERIEQQLQKI
                     ENGDGLPD"
BASE COUNT         3664 a         5967 c         5706 g         3651 t
ORIGIN      
        1 accctcccgc cagcatcgca tccaccacgg gcgcgaacag caccgcctcc tgcaattgcc
       61 gcccccagta gcccgcgtca aactccgtcc cgacgcaggc actcccgctc agtgtagaaa
      121 agattggaat caaggcgcgg cgcgcttcga ggtcaaccag agaggcttcc agttcgtctc
      181 caaactcctt catctgcggg ctgtggaagg cgtagtcgcc gcgtaactcc tgagcctgta
      241 gtcctcgcgc gcgcagggct ttgacgatgt caacgagcgc cgcgctctcg ccggagagga
      301 cgcacgagcg gggtgcgttg tgcgccgcca ccgtcaggcg gttctgatag tctgcgagaa
      361 aaggcgcgac ctccacaggg tgaagtgcca ccgccgccat ctttcctccg ccggtcgcac
      421 gctgcatcag acggctgcga tggaagacga cgcggactgc gtcttccaaa ctcaacgccc
      481 ccgcgacatg agccgccgcc acctcgccga cactatgccc gacgacggcc tccggcttga
      541 tgccccacga ctgccacagc gccgccagcc cgacctgcaa cgcgaacagg catggctgcg
      601 ccacttcggt gtcggccatt cgtgactgct gctcggtttc ggccagcaac tcgtcgagca
      661 gcgaccaacc ggcgacgcgc gcgaacaact cgtcacaccg ctccagagtt tgacgaaata
      721 ctttctcatc gcgccacaac tggcgtccca tcgcccacca ctgcggcccc tgtccggcga
      781 agacgaaagc gagcgacgcg cgccggttgt gtgtcacgtg ccccgtggag attgccggat
      841 gaggcgtgtt ggcaaggaag ctttcgagcc gctccgcgaa atcttcgcgc gaggttccga
      901 cgagggttag acgatgttcg tggtgtgtgc gccgcacagc cgccgtatgg cagatgtcgc
      961 gcagcgaggc ggtgttggct tcgtcaagca ggaatcgccg gtaggcttct gccagagcgc
     1021 gcaacgcgtc aggcgcgcgg gcggagaggg gcaggagata ggctgcgtcc gttgaagttt
     1081 gtgtttcgac agagagcgcg cgctccgttt ccgggtctgc gccgacgtgt ggagcttcac
     1141 ttacgggcgg ggcttcgctg acgatcaggt gagcattggt tccgccgaag ccgaaggcat
     1201 tcaccccgac caaggccgga cttattcctt ccggccacgg agccagagac tgttggacgc
     1261 gcaggggcag ttctgcgaaa ggaatatgcg gattggggtt ttgatagtgg agactcggag
     1321 gaatctcacg atgcttgagc atcagcacca ccttcattag ccccgcgact ccggctgccg
     1381 cttcgaggtg cccgatattg ctcttgattg agccgacggc gcagaagtct ccctcaggcc
     1441 ggtcggtgga gagcaccgcg ccgagcgcct gagcctcaat cgggtcgccg agtgaagttc
     1501 cggtgccgtg cgcctccacg tatgagatgt gcgcgggcga gactccggcc tgcacgcacg
     1561 cctcgcgtat gagcgcctcc tgtgcccagc gattcggcgc cgtcagcccg ttgctgcgcc
     1621 cgtcctgatt gaccgcgccg cctttgatga cggcatgaat cgggtcgccg tcggccagcg
     1681 cccgcgagag aagtttcagc acgacgacgc ccgccccctc gctccgcacc agcccgtcgg
     1741 cgcgtgagtc gaaagtttta cagcgtccgt cgcgtgcgag gagtccggcc ttggcgtacc
     1801 agatggttcc ttccggcgaa agaatcagat tcgcgccccc ggcgatggcg agcgtggact
     1861 cgcccctcca caaactttga cacgccagat gcgtcgccac gagcgacgac gaacaagcgg
     1921 tgtcgatgga catgctcggc ccgcgaaaat cgaagaagtg cgagatacgg ttggaagcga
     1981 tacatctggc gtggccggtg acggtgtaaa agttgacgag cagcgggtcg ttcatcggcg
     2041 tgtcggcgta atcgctgccc atgatgccga cgaagactcc ggtgggactg ccagccagtt
     2101 ttcccggcac ctgcccggca tcctccaacg cttcccacgt cacttccatt aagagacgct
     2161 gctgcgggtc gagatgtatg gcctcgcgcg gtgatactct gaaaaagtga gcgtcgaagt
     2221 gatcgatctg ttcgaggaag ccgccccagc gtgtgttcat cttgccgggc acggtcgcgt
     2281 cttcatcata gaaacgatca acgtcccaac ggttcgcggg aatctccgtg acggcatcca
     2341 cgccctcgcg taacaactgc cacaattccg ccggactgtt cgcgcccgga tagcgacagg
     2401 ccatcccgat gatagcgatt ggctcggtca gttgtccgcc ggaggcagaa cgtgccggat
     2461 cgtttggaat attcttcatg ctcatctaac tcctttgttg gaaccccttg ctccagtgct
     2521 ctagcctctc cgctcatgca tcgcggttga cgctttgtga ccgcgcgagt tgcaggggca
     2581 cgacggagaa caaacgctcc tcttcataga gccgctccaa ctcttccgcc actcgctcct
     2641 cgtgttctgc gggtaacacc ctgcgccgct ctcccaaccg caccagcaat cgctccagac
     2701 tcaacaccag ccagtccccg cgcccgaaga actcgcccaa cgtctcacga ttatgcagcc
     2761 atagatgcac acagcaggcc gccgcttcca gcaggcaata tctctccgcc tgctcgaaca
     2821 tctccggcgc acggctgtag ttgttgccca actgtgcggc gagcgcgcgg tgctcgtcat
     2881 cggcggcctg tcgctgcgcc agcactcgcc cggtctgtgc aatcaggcat tgcagcaacg
     2941 ggtctgcgtc cggtgtgtgc ggagaaagaa gacgaagttg tgacagagcg aggtcgaggc
     3001 cattgaggat gtcgtcgcgt ccgcgagtat agaggctcag cccgtcggga ttgaagtcgg
     3061 ccagcgggtg tcgcaggtcg aagatggagg ccagccgcat tgaaagttcg gcagcgtcag
     3121 tatcagcctg cggagcggtg cgcgcgcgat gttgcgcgag atcgcgcagg tgcacgccga
     3181 tggttgttaa gttaagcagt gtcccggtgt ggaagatgct caataccttg ttgtcccgga
     3241 gcaacttctg gaagatgccg tgccagtgcc cttctcgcaa gtaatggcgc gcacccagca
     3301 ccactcccag ccgctctatc gtctcctcca aactctgcgg cacgtaatac ttcaccactg
     3361 ccgaatacag acgcatctgc tccggcgtct ggtgcatggc tctggcgcag gccgtcgtca
     3421 ggcagtcgcc gatcagcagg tcgaggaagg cttcgacgag tgtgcgtcgt gcttgcggca
     3481 gggcggcgac gcgtgtgccg tagagagcgc gtgtgcgtgc gaagtcgagc gtggagcgca
     3541 gggcggtgtc ggctgcgccg agcgcgaggg cgggaatgat gatgcgcgtt agttgaaagg
     3601 ctttgagcgt ctgttccagc gcggagcctt ccctgccgat caacgagtcc tcgctcacga
     3661 aactctccct gaagcatatg ccgctaaagt ccacgcccct gacgccgagg gttttgattc
     3721 gcggcagatg ttcgaaagag gacgggtcga gcgcacgctt gtctaccaga aacagggaga
     3781 atccgcgtgg tccaccactc tcggctgttc tggcaaagac ggtcagcccc acgccacgcg
     3841 tagcattgtt aacgagccac ttttcgccgg agagtcgata gccgcccgcc accctgacgg
     3901 ctttcacgtc cgtcgccagc acgtctcccc cgtgcgcctt ctcgtgatat gcgagggcta
     3961 cctgctttcc ctccatgacg attccggcaa gccacttccg ctgctccggc gagccgccag
     4021 tccaaacatt caccgtgccg agaaaggact tagcgtgcgc cactatcacg ctcagattgc
     4081 gtcgcgcgac gacacgcaag agggagatca actcttcgta ggaacgcagc cgcccgcccc
     4141 actgcaccgg cacgtagaag tccggcaacc cccacgcatc caacaacgca caggcttgct
     4201 ctggatactc ttctccttca tcaagcgcca cggcctgcgc gaacgagaaa ccgttgcgcc
     4261 cgtcctgcgg gtcgcccagg tatccttcaa gttcttcggc catgccgtat tgctccggta
     4321 tatgcatcgc ttctctcgct gcttctctcg ccctgtctcg ctccttaact tttctcgcac
     4381 cttacctgtt ggccgccgcg tcgcctgctg cttccgcgct caggtggcgg gaaagtatct
     4441 cgatggtcgg gaagtcccag agcagggtcg ccggaagcgg acgccgaagg aagtcctcaa
     4501 ggtcgccgga caggctcacc gcgtctattg agttaagacc gaaattggcg aaaggctcat
     4561 gcacgtccac ctcttcggga ctcaccctca tcaactccgc aagtcttgag atcagccaac
     4621 tctgtatctc ttcaaggctc gcgcccttct gcgcgcccgc cgctccggca gagcgcccgt
     4681 cgccatccac agttcccatc tttgaacctc caaaaccgaa aatatgatta ctacaaattc
     4741 gctgccccgt gccttcaacg caccacagat aacgaccgtc agttgtgcga cgcgagttgc
     4801 aggggcacga cggagaacaa acgctcatcc tcataaagcc gctccaactc ttccgctacg
     4861 cgctcctcgt gttccgccgg taacaccctg cgccgctctc ccaaccgcac cagcaatcgc
     4921 tccagactca acaccagcca gtccccgcgc tcgaagaact cgcccagcgt ctcacgatta
     4981 tgcagccata gatgcacaca gcaggccgcc gcttccagca ggcaatatct ctccgcctgc
     5041 tcgaacatct ccggcgcgcg gctgtagttg ttgcccaact gtgcggcgag cgcgcggtgc
     5101 tcgtcatcgg cggcctgtcg ctgcgccagc actcgcccgg tctgtgcaat caggcattgc
     5161 agcagcgggt ctgcgtccgg tgtgtgcgga gaaagaagac gaagttgtga cagagcgagg
     5221 tcgaggccat tgaggatgtc gtcgcgtccg cgagtataga ggctcagccc gtcgggattg
     5281 aagtcggcca gcgggtgttg caggtcgaag atggacgcca accgcgccgg aagttcggcg
     5341 gagatagcat cgttagtctg cgaagcggtg tgtgcgcgat gctgcgccag atcgcgcagg
     5401 tgcacgccga tggttgtcag gttcagcgcg gcattgaaat gatacctgac gaccacgttg
     5461 tccctgagca acttctggaa gatgccgtgc cagtgccctt ctcgcaagta atggcgcgca
     5521 cccagcacca cccccagccg ctctatcgtc tcctccaaac tctgcggcac gtaatacttc
     5581 accaccgccg aatacagacg catctgctcc ggtgtctggt gcatggctct ggcgcaggcc
     5641 gtcgtcaggc aatcgccgat tagcaggtcg aggaaggctt cgacgagtgt gcgccgcgcc
     5701 tgtggcaggg cggcgacgcg tgtgccgtag agagcgcgtg tgcgtgcaaa gtcgagcgtg
     5761 gagcgcaggg cggtgtcggc tgcgccgagc gcgagggcgg ggatgatgat gcgcgttagt
     5821 tgaaaagctt tgagcgtctg ttccagcgcg gagccttccc tgccgatcaa cgagtcatcg
     5881 tccacgaaac tttctttgaa cctgatgccg ctgacatgtg cgcccctgac gccgagggtc
     5941 ttgattcgcg gcagatgttc gaaagaggac gggtcgagcg cacgcttgtc tactaagaac
     6001 agagataggc tgcgcggtcc ggcttcgtct ctggtgcgcg cgatgaccgt caacgcgacg
     6061 cagcgcacag cattgccgat caaccacttt tcacccgata gttgatagcc gcccgccacc
     6121 ctgacggctt ttacatccgt cgccaggaag tctcccccgt gcgccttctc gttgtaggcg
     6181 agaatgatct gctgtctgcc ctttagaagt tgcgccacct tctgtttttg cctgtccgta
     6241 ccacccgccc acgtcgccgc cgagccgagc agtgacagga tgctataagc aaatccgaca
     6301 gtgaaatccc gccgcgcgac gacacgcaag agggagatca actcttcata ggaacgcagc
     6361 cgcccgcccc actgcaccgg cacataaaag tccggcaatc cccacgcgtc caacaacgca
     6421 caggcttgct ccggatactc ttctccttcg tcgagcgcca cggcctgcgc gaacgagaaa
     6481 ccgttgcgcc cgtcctgcgg gtcgcccagg tatcgctcaa gttcttcggc tgcgccgtat
     6541 tgctccggta tatgcatcac ttctctcgct gcttaacttc tctctcgccg cttattttgt
     6601 tgcttaagtt cgctcgtcgc ttagcgggtg gccgccgcgt cgcccgttgc cgtggaggtc
     6661 tttcaaaact tattaatgga ctcccgaaac tgaaaatgtc tcacagacaa ctcctctgcg
     6721 ctgtgccttc gtcgcgctgt gcatacggca ttggcagtca gtcgtcagtt gcgcggcgcg
     6781 agttgaaaag gtacgaggga gaacaaacgc tcctcctcat agagtcgctg catctcctcc
     6841 gccacgcgct cctcgtgttc tgcgggcaac accctgcgtc gctctcccag ccgcaccagc
     6901 aaccgttcca gactcaacac cagccagtcg ccgcgcccga agaacccgcc caacgtctcg
     6961 cggttataaa cccacaggtg cacacagcag gccgccgctt ccagcaggca atatctctcc
     7021 gcctgctcga acatctccgg cgacttgttg aagccgctat ccctgagcga tgccgtaatg
     7081 aactcacgct ggctctcgac ttcggacagg atcgcccgcg cacggctttc gatcagcgcg
     7141 agggttgcgc cgtctcccgt ctctttcggc tcgatagacg aaagtttttc gagcgagagt
     7201 gcgaacccct gtaggatgga atcacgcccg cgattgtgca gcgtgagcgc ggcaggatcg
     7261 aagtccggca gcggctccgt caacgaaaag atgcgtgcga gttgattcga tagctcggcc
     7321 tctctctcct gcgtggattg gctgccgcgt gtgagatgtc cgagttggat agcgatagca
     7381 tggaggttaa cgacggtgct gccgtcgaag agactcagaa tggcgttgtc cctgtagact
     7441 ttttgaaaga tgccctcgca atgttcctgc cggagatact ggcgcgcgcc taagacgatt
     7501 gacaggctct tgagaagttt ctcaaccatc gtcggcacca gatacttgct gatggaagag
     7561 cgcaggctca tctgttccgg ttcgacatgt atggaacgcg cggaggcgac ggtcagacat
     7621 tcgcaaatca agaggtctat gaaggcatcg cagagaaggc ggcgcgcgtg tgggatggca
     7681 aacaccgaat cgccgtagag cttgcgcgcc agtgcaaact ccaacgccac gcgcaaggct
     7741 gtgtcggcgg ctccgagcga caggccggtg atgacggtgc gcgtgagttg gaaggctttg
     7801 agcatgatct ccagccctgc cccgacctga ccgatgacgg aatctgcggg caggaaagtg
     7861 ttctcgaatc tgatgccgct gatgtctgcg cccctgatgc cgtgcgtctt aatcttcggg
     7921 agcgtccggt aggtcgagcg tgcgccgtcg cttttctcca ggagaaagag ggagaagccg
     7981 cgcgggtctg aggcggtgcc ggttctggca aagaccgtca gcgtgcgggc gcgcgtcgcg
     8041 ttgttgataa gccacttctc gccgctcaac ttataaccgc ccgcgatgct cccggcttcc
     8101 acttccgagg ccagcaggtc gctgccgtgt gatcgctccg tcagcgcgag ggcgacttgc
     8161 gcgtcgctcc tgataatctc cgcgagccgc tgctgctgct ccggcgagcc tccgacccac
     8221 gtggcgatgg ctccgagata ggtcttgccg tgggcgatgg cgacggtcag gtcgcgtcgc
     8281 gcgatgacgc gcagcacgtt gatgaactcc tcgaaagatg ccaactttcc tccgagcgtc
     8341 gaagggatgt aatagtccga gactttgagc gcgtcgagca gttgacaggc ttgcgccgga
     8401 tactcctcgc gttcgtcgag cgcgacggcg ttcttgaagg agaaggcgtt ctccggcgcg
     8461 aacggatcgc cgaaaaggcg ttcgagcgtt tccgccgcgc ggtgttgtgg gaaagtctgt
     8521 gtctgcatca ttcggagaaa aaggtttcgc tgctcaatta aacggaagac gttcgagcct
     8581 gcccgtcaga taagcctgac gacatgcctg acgcctgatc ttgccgctcg aagtcttcgg
     8641 gatgcccccg accttgagcc acaccaactc atgcacatga agcccgaaat aatcggcgat
     8701 agcccgctgc acgccggtct tcaacagttt ccactcctca gccgcgaagt gtctggaggc
     8761 tgcgcccgcc gtctcccccg tcggcgcggt gcgcttgtaa cgctggtcaa cctccgccgc
     8821 gatgaccaga ctttcttcac cttcaaactc gacggagaat gccgccacgc atccggaacg
     8881 cagcgagggg tgacactgtt cgacggttag ttcgatgtcc tgcgggtagt gattgcgccc
     8941 gccgatgatg atcaggtctt tgaggcgtcc ggtgatgaac aactccccat ccttgatgaa
     9001 acccagatcg cccgtgcgga gaaagggtcc ctgtcccgtg tccgagagat gcgctttaaa
     9061 ggtctgctcg gtcaactccg gacggttcca gtagccttgc gccacgcccg cgcccgacac
     9121 ccagacttcg cccacgctcc ccttcggaca ttccctcagg ctcgcaggct cgactatgac
     9181 ggcgcgtgtt ccgagcgaca cgtcgccgct gccgacgagc gttctgacgt tctgcgcgcc
     9241 gggcgcggcg gtggaggctt cgacgaggag attttcttcc agcggcgcgg cctccagttt
     9301 gcaggtcacg ggaggcttcg cacctttgcc gcccgacacc ttgagcgtgg cttcggccag
     9361 accgtaggcc ggataaaacg catcccagcg gaagccgcac tcctcgaagc tcgcggcgaa
     9421 tctccgtaga gtctcctccc gaatcggttc cgcgccgtta taagccacgc gccagcagct
     9481 aaggtccaga ccgccgcgct gttcggggcg aatcttgttc acgcagtgtt cgtaagcgaa
     9541 gttgggaccg ccgctgtgcg tcgcacggta acgggagatg gcctgtagcc aacgcagcgg
     9601 gcgttggagg aaggcgaccg acggcatcag gtaacacgtg aagccagcat agagcggctg
     9661 catgatgccg tcgatcaatc ccatgtcgtg ataagcgggg agccacgtaa cggagatgct
     9721 ctccggcgtg tgctcgaatc cgtagtgaat gtaagcggag ttctccaaca ggtttcgatg
     9781 actgaccatg acgcctttcg gggcgttcgt ggagccggag gtgtactgta gaaacgcgag
     9841 gccgtccgcg tccggctccg gttcgttcca gtcttccgca cgcccatgct cgatctcgtc
     9901 ggtcgccagc catctcaggt gtttgaagtc ttcggtgcgg gcgaagagcg gcgcaacttt
     9961 cgcaaggatg ccggtcgtcg tcagcacaaa tttcgcctgc gagtcggcga gaatggagtg
    10021 caacctcggc agcgttattt caggacgcgc agggtgcggc gggtaggccg gaacggcgac
    10081 cacgccgctg tagaggcagc cgaagaaggc ggccacgaag tcgagtcccg gcgggtagag
    10141 cagcagcgcg cgctcgcccc gcgcctgcaa actacagagg gtcgcgccga tgccgcgcgc
    10201 gagcagatca agcccgctat aagtgacgcg ggcttgttcc tgctctccgt cgccgagata
    10261 cacgtaggct gtgcgcgccg gttggtctga cgcgcgcagg cgcagaaagc cgtcaatggt
    10321 ggagggttgg agtttcaatt cgactcgacc ttaagtgtca gtttgcaaac cgctgtctac
    10381 tcgtatttca gtgcccgcag cggatcaatt ttggtagccc tgcgcgccgg aatgtaagtc
    10441 gccagtaaag cgatcaccac ctgaaagagc gtgatcgcgg cgaaggtagt gaggtcggtg
    10501 gcgctcactc cgtagagcag tccggcgaga aagcgcgtcg cggcaaaggc caccacgagg
    10561 ccgacgccca atccgatcag ggtgagcagc atgccttgtt taagcaccag tttgagcacg
    10621 tcgccgcgct gcgcgccgag cgccatgcgg atgccgatct cgcgcgtccg gttgctgacc
    10681 gagtaagcca tcactgcgta tagacctatc gtggcgagca gcagcgcgag caggctgaag
    10741 atactcagga gcacggcggc caacttttgc tggatgagca cggttccgag cgcgtccttc
    10801 atggtgatga tgcgcgcggg aatagtctta tcgagcgtcg ccacttcctg ccggagggct
    10861 ggcagcagcg tccccatggc tccgttagac ttggcgaaaa ctgtcatcct cgcggagtag
    10921 ctctgctgga acggcaggta gaagtatgga atcggctcct ctttcaggtt gtagtacttg
    10981 gtgtttttca cgaggccgac gatctctatc ggcgccttgc ccccgacgag gctgaactgc
    11041 tggccgatag ggttcttgtc cggccaatag cggcgcgcca tcgcctcgtt gatgatgcag
    11101 acgccgggtg cggtctttgt atcgaaatcg gtgaagtcgc gcccctgcac gaacggaacc
    11161 gacatgacct gaaagaagtt gcgcccgacg atgttgaaat caaccgccaa ctgcgtgttg
    11221 ggcggcggct cgtagcccgg aatcttaacg gccatctgct gcatcccgcc gccgctgagc
    11281 ggcacgacgt aggcgaggct ggccgtttcc acacccggca gttggctgac gcgctccatg
    11341 agttgctggt aataacgctg tccgttttcg cggtcgtaac cttgcagtcc cacgtccgta
    11401 gagacgattg ccagtttctc cggctcgaaa ccggggttgc tgttctgcgc attctggagg
    11461 ctgcgcagga agagtccggc gcagatcagc aggatgagcg aaatggcgac ctgaccgacc
    11521 accagcagat tgcgcagccg cgacttctta tagccctgcc ccgatccctc gtccttgagc
    11581 gccgggatga ggtcggtctt ggtggtctgg atggccggag cgaggccgaa gatgatgccc
    11641 gtcaagaccg agacgagcag agcaaaaatg tagacttgcc ggttcaggct gaggtcgaag
    11701 gagacgggaa tcgaggtggg cggaaccaga ttcaggagca gcttggtgga gatgaaagcg
    11761 atcaacagcc cgacgagtcc gccgagcagg gagaagagca cgctctccgt caacaactgc
    11821 tgcatgagcc gcgtcctcgt cgcgccgagc gcctgacgaa tgccgatctc cttgtgtctc
    11881 gccagagcgc gcgcgagcat cagatttgcg acgttgaagc aggcgattaa cagcaccaga
    11941 aacacgaccg ccatcagcag ccagagcacg ccggacatgt tctttttgat tccggtcgaa
    12001 ccttctccga gcggcacgac ggttggcgtg gtcgccttat tcgtgccggg atgctcctgc
    12061 gccagacggt tataaatacc ctgcaatccg gcttcggcct gttcccggct gacgccgggc
    12121 ttgaccctcc cgaggacgtt gagccaacgc gcgccgcgat ccgtgaggcg gtctgatccg
    12181 ggcagcacac gcttctgcat catcagcggg aaccagatgt cgggcgcgag tccgatgatc
    12241 gtcccgatga aaccttcggg cgctacgccg ataatattga aactttgcga gttgacgatg
    12301 atggtcttcc cgacgatgtc gggcgcgctg ccgaaagcgc gtcgccacag ggagtggctg
    12361 ataactacga ccggatgaga attaggggtt tgatcttctt ccgcagagaa gccgcgtcca
    12421 atcaccggct tcacgtcgag ggtgttgaag tagttgccgc tgacgatggc gctcgccacg
    12481 atctcgttgt tcccgccgtt gctcaggctg acgggtgtga cccgctgcgc gatcatccct
    12541 gagaacacct gattgccgtc gcgataatct acataatcag gataagagac agccccgaag
    12601 cgtagacccg gcgaactcgt ataaacttcg aagagttgct tctcctccgc gacgccgggc
    12661 agcgggcgca gcaggatggc gttaatcaag ctgaaaatcg tgatattcag gccgctaccc
    12721 agaccgaggg agagtgcgac cacgagcacg aggccggggg tcttaaacat cgtccgcagt
    12781 ccgtagcgaa tatcttgcag caggtttttc atagtatgaa ctccaaatct ttagcgaaaa
    12841 ctctatggca tcaaatgatc tgacaggtta acgatatggt tcgcagagcc gctcccgcct
    12901 tttctctcaa agcgcgggct ggctcgcgtc agacgagggt tgggaattga acatcaggct
    12961 catgcaggtc agcgcgcctt cggccttttg taattcggaa atgtcggtgg tgatgacttg
    13021 aaagccctgc gcttcgatga gcgcgcgcgt gcgctcgaag gggcttggca ccagaaccac
    13081 gtcgccgacg gcgatggtat tggcagccca cggctccgtg tccggcactt cgagcagttt
    13141 gaaataacgg aacgcgctcg aatcaatcca cgccgaattc accaacatca ggtcacgccc
    13201 gagataggag catcccgtgg agagatggag gcagcccttc accccgaccg ggctgacctg
    13261 atagtcgtag ggtgcgagaa tctcactcaa ctgcctgatg ccttctcgat tggtgcgccc
    13321 cgaatcgccg acgtaaagcg tccgcccgac gcgcaccacg tcgccgccct ccagcgtcgc
    13381 cggaggcgag agatacctga gcgggcggtg agatgaaagc gcgccggtca cgctctccac
    13441 ctcgccactc cggcacgccg cgcccatcgt cgccatgatc gccacttcgt caaccacgac
    13501 cgccgtgtcc tgtatgaaaa ccgcgtcggg ggcttcgggc atgagcggca gcggggtgac
    13561 tcgcacgccc atctgttgca ggcaccgctc gtaggcttcg tgctggcgcg ttgccgccgc
    13621 aacgtccact tgttcgcggt gcataaaggt cagcttgcag tcgccgatgc tcggactgac
    13681 accgcgcgtt aaggctgaga gcataagtat tccgtctcct cgtacggggc gtttatcaga
    13741 gtgaagcggg cagcgtggag ggcgcgtagg ctgagacgcc caacagcgca tcctccacga
    13801 tcttgccctg ttccaacttg atgatccggt ctgccatgct gaaatagcga tcatcgtgac
    13861 tgatgacgag aatcatcttg cctctcgcct tgagttccgg caggagatgc aggtagaaga
    13921 actgcttgaa gagcgggtct tggtcggctg cccattcgtc gaaaatgtag acggggcggt
    13981 cgtcgagata agcggtcagg agcgcgagac gtttgcgctg gccttgcgag agatcggtcg
    14041 tcgagagcac gccgtccttg accttcactt tatgatcgag ttggagcttg gagagatagt
    14101 cgcccgcctg ttgatcgacg gtgagccggt tgtgacccat cagacgctcg aacaggtgcg
    14161 gcgtcgagaa gacgatggag aagagttgcc ggtaggattc tctgttctgc tcggtgacgg
    14221 gtgtgccgtc gaatctgatc tcccccgatt cggggatgta aagacccgtg atcagcttgg
    14281 cgagagtcgt cttgccgctg ccgttgccgc ccacgagaaa gacgagttca ccctgtcgca
    14341 aggtcatact cagaggtccc agcagaaagt cgccgtcgcc gctttcgtgt ttgtaaacgt
    14401 gtgtcacttc ggacagttcg agttgctggc aggtgggttc cggatcgacg gcagcctcgc
    14461 tcacggcgtc ggtgcctgca aacgataagc ccgtctcttc tactttcgtc agagagatgt
    14521 tggcattatt gaaagaaggg ataatgtcga gcacgatggt catcggcgtg cgcaggtaga
    14581 gcgccgccag cgcgtaggcg atcagggtgt cgctgctgat cggccacagg cgcggcaggc
    14641 cgtagacgag cagaccgatg aagatgaaat ataacgcctg cgatccggcg gcgttgacgc
    14701 cgagcatttg ccgcccgatg ctgttattcc gccggaaaga atcggcgcgc ggcatcatga
    14761 gttcggagaa gaaagcctcc cggcgctgcc ggttcaggcg cagttctttc gtcccatcga
    14821 gcagcgagcg aatatggttg agcagtacgt cccaatcgtc gcgtgccctt ttcagatgac
    14881 gcccggctgc ccggttcacc agttgtaagc tggtcatggt aacgatcagg ataaaggtaa
    14941 acgccgcgaa cactatcggg gagagccagc agagatagat cagacagccg acggccatcg
    15001 ccagattgac gcagaaaacc ggaaaatcga ggagcgcgat ggcgatagtg gacatgtcct
    15061 gcgtgagtat ggcgaggatg cggtgagagc ctatctcctc aaatttgcgc aaagggacgt
    15121 tgaaagcctg acggctcaaa cgcaggcgca actcatacgg cacctgctgc gagattttga
    15181 tgagcagaaa tctcgaaaag agattggcga gcaccgagac gacgagcagt ccggcaaagt
    15241 accaggccag cgtgagagac gcaggaggct catcgcgcag cgcgaccatg agcagtgcca
    15301 gtagtcccgc actactgatc ccggcgattg ctccggcgaa ggccgcaagg ataaaagtgc
    15361 gcttcgagta gcgaaagatg aaggagatta ttttcattgt ttgtgcgtcc tccggcaaaa
    15421 tccggtgaga aggtttaagg ttagaggacg ccccgcaaag tttgcctctg aaggcagcgc
    15481 ccgccgtaat gcgatgtctc ggatcaatca ggcaatccgt cgccgttctc aattttctgc
    15541 aattgctgtt cgatgcgttc cgctaaagcg tcaacatatg gttgcttaag gatggaatag
    15601 tggtcgccgg ggagcctgta gacctcaacc ccgccgtgcg tcagttcgtg ccagccttgc
    15661 gacatgtcgc ccgcgccgtt cgtgccgtcg ccggtggcct ggaagtaagt gacccgcccc
    15721 gcgtagacgc ccggcacata cccctgcatc gcttcccgat tcgtcttgaa gacactgaac
    15781 aggcgatgca aatgggagag ttccaagccc gcggggacaa gcccttcggc tgacgccagt
    15841 tcgagcatgt aggccagttg cccttcaagc ggcagcgacc gcaccgcgtc tctcgaacgc
    15901 gacagatgtg ccttcgtgaa tcccaggtca agcgcgaagt tcgccagttg cgacagctcg
    15961 tcttccggct ccattccggc gagtgctgac ggcgagagcg aatcaatcag cgccagcatg
    16021 acgacttcat ctccgcccgc tttcaattgc cgcgccatct cgaacgccac cacgccgccc
    16081 agcgaccagc cgccgatgcg atatggacca gtgggctgga tggcgcgcag gtgttcgatg
    16141 tagtagctcg ccatctcctc gatccgcgtg tggccgtgct gctcgtcaga gagtcctctc
    16201 gactggaggg cgtagaacgg ttggtcgtcg cctaatctgc gcgcgaggtt gaagtaacag
    16261 aacacgttgc cgccaaccgg atgaacgaag aacagaggcg tcttacccgt gccgcgctgg
    16321 agttccacca gcggcgacca tgaaccggcg acaggctgta acgcgttcag gagggaagac
    16381 gcgtcgccgt cgtcgtctcc gcagaccgca gaaaccgtgc gcccgtaatg ctccttcaaa
    16441 tgcgcggcta aggctgcgac gttcggagcg ccgaacaggt ctttgactga caccggaact
    16501 cccaaacgct gctggagccg gttgacgaac accacgacgc ggatcgaggt gccgcccaga
    16561 tcaaagaagt tgtcatacac gcctaccgtc tccactccca acacgtcttc ccacatctct
    16621 accaggaacc tctccaaacc cgtccgtggc ttctcataac ggctctccaa ctcagggcgt
    16681 gagccatcag ggtcaggcag cgcacggcgg tctaccttgc cgttcggcgt caacggcaac
    16741 tcttccagca ccaccaccgc cgacggcatc atgtactccg gcagacgctc ccgcagcgcc
    16801 actcgcaact ccgcccaccc cggcgcgcgc tccccttcca cgcccaccac atacgccacc
    16861 agccgcttct ccccggactc ttcctcacgc accgccgcca cgcactgcct cacaccctcc
    16921 accgcacaca ccgccgcttc cacttctccc aactcgatcc ggtacccacg caccttcacc
    16981 tgctggtcgc ttctccccag aaactccaac tccccgcacg cacgccatcg cgccacgtct
    17041 cccgtacggt acatccgcgc tcccggctcc gccccgtgcg ggtctgctat gaaacgctcc
    17101 gccgtcaact ccgcacgatg caggtagccg cgcgccagcc cctctccgcc gatgtagagg
    17161 tcacccgtca cgttgagcgc gaccggctgg aactgttcgt ccagcacgta gacgcgcgtg
    17221 ttgtctatcg gacgaccgat ggtgacggcg cggtctgccg ggctgatgcg tttgagcgtt
    17281 gaccagatgg tagtttccgt cgggccgtag agattccaga ccggcgcacc tgattcgagc
    17341 aggcgttcgg agaggtcgcg cggcaacgct tcgccgccgc acaacagggt gagggtgccg
    17401 tcatgctgcc agccggtgtc gaggagcatg cgccaggtgg cgggggtggc ctgcatgacg
    17461 gtggcttgcg tctgggccag acgttgttgc aggcgcaggg catcgctggc ctcctcgcgc
    17521 gaggccagcg cgagacgcgc gccgcacacg agcgggaggt agagttccag agcggcgatg
    17581 tcgaaggaga gggaggtgac ggcgagcagg gtgtcggcgg cggacaagcc cggctcacgc
    17641 agcatggaat gaaggaagtt gaggagcgag cgatgggtga cggcgacgcc cttgggtctg
    17701 ccggtcgaac ccgacgtgta gatcacgtaa gccagattgt ccgcctctat gctgacctca
    17761 aggttcgggc attccttcgt ctgctccgtc accacgccca gccccagttg ctccacgcac
    17821 accaccgtcg cagtttccag ttgcggcgca tcagcccact tgccgagtaa ctcttcgttc
    17881 gtgagcagga cgcgcggcgc ggcatcctcg aacatatagc gcaggcgttg cgccgggtaa
    17941 gcgaggtcga gcggcagata ggctccgccc gccttgagca cgcccagcag cgagacgacc
    18001 atctccaccg aacggtcgaa cagcagcgcc acgatgctgt cggaggtgac gccgagcgtg
    18061 cgcaggtgaa gggcgaggag gttggcgcgg cggtttaact cgcgataaga gagttgctgc
    18121 ccgccgcaga tgagcgcgac ggcatcgggg gagcgttcga cctgtgcttc gaagagatga
    18181 tggaggcagc gttcgagttc gtaggggcgc gcggtcgtgt tccactccac caactgctgc
    18241 tgccgttcct cgtcgctcag catcttcaag tcgccgagct tcccttcccc ccgctccgct
    18301 aatacctctt tcaccaccct ctccagatgc ctcaccaact gctgcatcaa cgcctcggag
    18361 tacaactccg cacggtattc cagcttccct ctgactcctc ccccgcgttc ctccgtcagc
    18421 cccagcagca cgtcgaactt cgcccctaag actccgccac ctgctccttc cgcactgacc
    18481 tccaagcctt ccagttgcag ccctgcatct ccaccgcctg cttgcaagac cagcatcact
    18541 tcgaacagcg ggtggcgatt caactcccgt gtcacgtcca actcttcgac cagcttctcg
    18601 aagggcacat cctgatgcgc gtacgccccc aggcacactt cccgtacccg ccccagcaac
    18661 tcgcgaaacg tcaactcccc gcgcaactcc gtgcgcaagg ccagcgtgtt gacgaagaag
    18721 ccgagcaact cctccgtgcc gtgatgacgg cggttagcca cgggtgtacc gacgacgatg
    18781 tcgggttgtc cgctgtagcg ccagagcaca acctggaaga cggccagcaa gctcatgaac
    18841 aaggtgcagc cctcttgctg tcccagctca cgcagcctcg cgaccgtagc ctcggacaaa
    18901 cgcacctcct gctgggcgct ctgtgagctg agcacggcgg ggcgtgctcg ctcggtaggc
    18961 agttccagtg cggcgggtgc ttctgcta
//
LOCUS       DASWNS010000227         5580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_242893, whole genome shotgun sequence.
ACCESSION   DASWNS010000227 DASWNS010000000
VERSION     DASWNS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242893"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..566)
                     /locus_tag="VGB76_07800"
     CDS             complement(93..566)
                     /locus_tag="VGB76_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098842.1"
                     /translation="MSVDGKREVLRHVVATVAYRGGIAVWGAPEDFAVFRVDKDVRAP
                     GEILAHIGDLLEGSLYLLRGEMVYLTSSPLPWGEEVSRFFSAAQRLDAYLRSSAPLAC
                     PVEKLIQGPIGDALTHVGQIVMLRRVAGSPVCAESYFTADIIPGMVREESFKKAD"
     gene            1249..3123
                     /locus_tag="VGB76_07805"
     CDS             1249..3123
                     /locus_tag="VGB76_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098843.1"
                     /translation="MKKLFYPFLALTFALASCVVSVTAQTPATAKVSPVADARLFPLE
                     DVRPGMKARAWTVFSGTEAQEFGVDILGVLPGFPAPRQSAIIGRLTGANAEKTGVFAG
                     MSGSPVYIDGKLVGAIAFSFPFSKEPICGITPIKQMIDIFERQQGTARTGSGTPRTMS
                     FAKLASTDWQADWPKPSILGGTFAAPVAAGSPLNALLGQTIAPIATPLVFSGISAEAL
                     SLYAPQLQANGLLPVAGVGGSASITPMGKVTEKTLPPGSSVSVQLVRGDYSLAAFGTV
                     TWRDNERIYAFGHPFLGLGIADMPMSESSVVTVISSVNNSFKLSSAGQMVGAISQDRA
                     TGIYGLLGRAPKMIPVKINLHTSRDRTETYTYEVANDKFLTPILLNITIFSTIGSTER
                     AIGESTISLRGKIDVAGQQPINIERRFSSMNAGAQTAASVAIPMAALLSSGFDNVDIG
                     QITLDITSTDERMSGTLDRIALDRTEVGRGETVEVQAYVRTDSGKQFVQRIPVKIPND
                     VPLGQLVLFVGDGGALQQASATQNFVPKDLTQLVGAINRIKKNDRLYVKLFRVTSGAV
                     IGTDEMPNLPPSVVATLNSDRTSGGFTPTALSPVDEKELPPAEFVIEGQQLISISVIR
                     "
     gene            complement(3232..3864)
                     /gene="rsmG"
                     /locus_tag="VGB76_07810"
     CDS             complement(3232..3864)
                     /gene="rsmG"
                     /locus_tag="VGB76_07810"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY0098844.1"
                     /translation="MKESLQEFEATLAKQAAHYGVPLGAQEIARLSAYYEHLLKWNAR
                     LHLVAPCAPGEFATRHVLESLMACSYMEEGGRVCDVGSGGGLPIIPCLILRPDISATL
                     VEAATKKVIFLREALRHLELQDRAQVLAQRFEQTPAPEADAVTCRALERFTEMYPRLV
                     RWSPPASRLLFFSGNALGELIARDENIRTFKAIRMPDSEQRFLFVCERKD"
     gene            3948..5459
                     /locus_tag="VGB76_07815"
     CDS             3948..5459
                     /locus_tag="VGB76_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0098845.1"
                     /translation="MSERLSAEHEQLGEIAAGIISRYARTLEEARVCSTATPGDLEKM
                     FDEPLPRAGIDSLQLFARFEREIAPHAMNISSPRYYGQFNPTPLPVAVWADALCSAIN
                     QNGAIWRNSPTASVIEARVIRWLCEIIGYGAESFGTLASGGSEANLIALKCARDRASE
                     FVRDKGLRAASWPPARTNGDASTQTATQGNMIVYASEQCHYSFVKSVDILGLGRENLR
                     KVATDERFHIRLDELRRAIEQDLEAGHLPICIAGAAGTTSTGVIDPLDQLADIAREYK
                     IWFHVDAAYGGALRMSEKYRDRLRGIERADSVTLDPHKWMFVPFAAGALLVREGGHVL
                     RDAFDITPEYLSEERGGQDVPFDFFRYGQLGTRRFNALKIWFALKTLGTDGYAEIIER
                     QIELTRYLAARIAELVDFECVGEVETAVCCVRFVPAEMRAAEVAEVNGVQQELQQRIE
                     RSGEAWLATTVLHGRRVLRVNVNSFLTERRHIDDLVELLRSESTQLWKARRAI"
BASE COUNT         1109 a         1763 c         1599 g         1109 t
ORIGIN      
        1 gccgcgtggc ctgctgcctg tttgaaggcg tcggtgctca tagcgggatg ataacattga
       61 gcgtcagcca catcctatgg aggacgatgc gcctaatcag ccttcttgaa agattcttct
      121 ctaaccatgc cggggattat gtcagcagtg aaataactct ccgcacaaac cggactcccg
      181 gccacgcgcc gtagcatcac gatctgcccg acgtgtgtca gggcgtcccc gatggggcct
      241 tgtatgagtt tctccaccgg gcaggccagc ggcgcgctcg accgcagata cgcgtccaac
      301 cgctgcgccg cggagaagaa tcgcgacacc tcctcacccc acggcaaggg agaggacgtg
      361 agataaacca tttcgcccct gagcaaatag agcgaacctt cgagcaggtc gccgatgtgc
      421 gccaggattt ctccgggcgc gcgcacgtcc ttatcgacgc gaaacacggc gaagtcttcc
      481 ggcgcacccc agacggctat gccgcctcga taggccaccg ttgcgaccac gtggcgcaac
      541 acctcacgtt tcccatctac tgacaaaaca ccgcctcgct ttcggacaat aagatttact
      601 cacccggaaa gccgcccaac gcctgaatta agccgcgccc cggagaactg cttgcttctg
      661 aaattacttt gatgaggatt tttgatgatg ggaattggga tttgtttgaa actgctttga
      721 atcgcgcgat tctcatttat aaagaactgc ctttgtttga ccagtacgaa aagtcagcaa
      781 aattatccaa aacttagcgg cttggtgtgc gctttctttt ggactctaat ttcggttttt
      841 tagccgcagc tttaggttgc ggcttttctt tattccaatt tgtgctttca gtttcctcga
      901 cggcgcgtgg ggctttctgt ttggtagtct ctggctctgc ttccagttca tcttaggata
      961 gaatcaacga cgctctttgc gagttcgttt tcgtctcgcg ttcatttgat cggcttcggc
     1021 atggggaaag tatgccacgc tccgatttca gatgctagaa gtattctttt cccggcaaca
     1081 cactattacc ccactaccaa cctttaagct gccttgacca tctgagaaag cggacgctat
     1141 agtcgcaaag gttctgcttc cgggcagccc gctgtcttaa aggtcgctgt tattgaggcg
     1201 cgaaggttat tgtcaccgat gcttcccccc tttacaaaag gtattcacat gaaaaaattg
     1261 ttctacccgt ttctcgctct tactttcgcg ctcgcttcgt gcgtcgtctc cgtcacggcg
     1321 cagactccgg cgacggcgaa ggtgtcgcca gtggcagatg cgcgcctgtt tcctctggaa
     1381 gacgtgcgcc ccggcatgaa ggcgagagcg tggacggtct tttcgggaac ggaagcgcag
     1441 gagtttggcg tggacatcct cggcgtgttg ccgggttttc ccgcgccgcg ccaatcggcg
     1501 atcatcgggc gtctgacggg agcgaacgca gagaaaacgg gcgtcttcgc cggaatgtcc
     1561 ggctcgcccg tctacattga cggcaaactc gtcggcgcga ttgcattcag ttttcctttt
     1621 tcgaaagaac ccatctgcgg catcacgccg atcaagcaga tgatcgacat cttcgagcgg
     1681 caacagggaa ctgcccgcac cggaagcggc acgccgcgca ccatgtcttt cgccaaactc
     1741 gcctcgaccg actggcaggc cgactggccg aagccgtcta ttctcggcgg cacgtttgcc
     1801 gcgcccgtcg ccgccggttc gcctctaaac gcgctgctcg gccagaccat cgcgccgatt
     1861 gccacgccgc tcgtctttag cggcatcagc gccgaagccc tctcgctcta cgcgccgcaa
     1921 cttcaggcga acggcctgct gcccgtcgcg ggcgtcggcg gttcggcctc gatcacgccg
     1981 atggggaaag tgacagagaa gactctgccg cccggttcct ccgtcagcgt ccaactcgtg
     2041 cgcggcgact attcgctcgc ggccttcggc accgtgacgt ggcgcgacaa cgaacgcatc
     2101 tacgctttcg gccacccctt cctcggcctc ggcatcgccg acatgccgat gtccgagtcg
     2161 tccgtggtga cggttatttc gagcgtcaac aactctttca agctctcttc agccggtcag
     2221 atggtcggcg cgatctcgca ggatcgcgcc acgggcatct acggtctgct cggtcgcgcg
     2281 cccaagatga tcccggtcaa gatcaatctg cacacgagcc gcgaccgcac cgaaacctac
     2341 acctacgaag tggcgaacga taaattcctg acgccgatcc ttttgaacat caccatcttc
     2401 agcaccatcg gctcgaccga acgcgccatc ggcgaatcga ccatctccct gcgcggcaag
     2461 atcgacgtgg ccgggcagca accgatcaac atcgaacgcc gcttctcttc gatgaacgcg
     2521 ggcgcgcaga ccgccgcttc cgtcgccatc ccgatggccg cgctgctctc cagcggtttc
     2581 gacaacgttg acatcggcca gatcacgctc gacatcactt cgaccgacga gcgcatgtcg
     2641 ggcacgctcg accgcatcgc cctcgaccgc accgaagtcg gcaggggcga gacggtggaa
     2701 gtgcaggcgt acgtgcgcac cgattcgggc aagcagttcg tgcagcgcat ccctgtgaag
     2761 attccgaacg acgtgccgct cggccaactc gtgctcttcg tcggcgacgg cggcgcgctc
     2821 caacaggcat cggccacgca aaacttcgtg cccaaagatt tgacccaact cgtcggcgcg
     2881 atcaacagga tcaagaagaa cgaccggctc tacgtgaaac tcttccgcgt cacttcgggc
     2941 gcggtcatcg gcacggacga gatgccgaac ctcccgccct cggtcgtcgc cacgctcaac
     3001 agcgaccgca cctccggcgg cttcacgccg accgcgctct cgccggttga cgaaaaagaa
     3061 ctgccgcccg cagagttcgt catcgaaggg caacaattga tcagcatcag cgttatccgc
     3121 taacagaagc atttaacgcg gaataccgga gagcacgcag ggggcgcaga gaagatcaat
     3181 tctctgcgcc cccttctgcg tctcttctct gcgtctctgc gttgaagttc ttcaatcttt
     3241 gcgctcacac acgaagagaa accgctgctc cgagtccggc atcctgatcg ccttgaacgt
     3301 gcgtatgttc tcgtcgcgcg cgattaactc tccgagcgcg tttccgctga agaacaggag
     3361 cctcgacgcg ggtggcgacc agcggacgag cctcggatac atctccgtaa aacgttcgag
     3421 cgcgcggcac gtcacggcgt cggcctccgg cgcaggcgtc tgctcaaacc tctgcgcgag
     3481 cacctgcgcc cggtcttgca gttcgagatg ccgcaacgcc tcgcgcagaa agatgacctt
     3541 cttcgtcgcg gcttcgacga gcgtggcact aatgtctgga cgcaggatca aacacggaat
     3601 gatcggcagc ccgccgcccg acccgacatc gcacacgcgc ccgccctctt ccatgtaact
     3661 gcaagccatc agcgattcga gcacatgccg cgtggcaaac tctccgggcg cgcacggcgc
     3721 aaccagatgg aggcgcgcgt tccatttcaa caggtgctcg tagtacgcag acagacgcgc
     3781 gatctcctgc gcgccgagcg gcacaccgta atgcgcggcc tgcttcgcca gcgtcgcctc
     3841 gaattcttgt aagctctcct tcatctcgtt tgtcggctgg aataaacccc tccacatagg
     3901 ttagcttaac cccacttcaa cttcatgcac aggaacgaaa aacggtaatg agcgaacgct
     3961 tgagcgcaga gcacgaacaa ctgggagaga tcgccgccgg aatcatctcg cgttacgcgc
     4021 gcacgctcga agaggcgcgc gtctgttcga cggcgacgcc cggcgacctc gaaaaaatgt
     4081 tcgacgaacc gttgccgcgc gcagggatcg attcacttca actcttcgca cgcttcgagc
     4141 gcgagatcgc gccgcacgcg atgaacattt ccagcccgcg ctattacgga cagtttaacc
     4201 cgaccccttt gcccgtcgcc gtctgggcag acgcactgtg ttcggccatc aaccagaacg
     4261 gcgcgatctg gcgcaactcg cccacggcga gcgtcatcga agcgcgcgtg attcgctggc
     4321 tctgcgagat cataggctat ggcgcggaga gtttcggcac gctcgcgagc ggcggctcgg
     4381 aagcgaattt gatcgcgctc aagtgcgcgc gcgaccgcgc ctcggagttc gtgcgcgaca
     4441 aaggactcag agccgcctcg tggcctccgg cgcgcacgaa cggcgacgcg tctacgcaaa
     4501 cggcgacgca gggaaatatg atcgtctacg cgtcggagca atgccattac tctttcgtta
     4561 agagcgtaga catactcggc ctcggtcgag agaatttgcg caaagtggcg acggacgaac
     4621 gcttccacat tcgccttgac gagttgcgcc gcgccatcga gcaagacctc gaagcaggcc
     4681 atctgcctat ctgcatcgcg ggcgcggcgg gcaccacttc gacgggcgtg atcgatccgc
     4741 tcgaccaact cgcagacatc gcgcgcgaat acaaaatctg gtttcacgtg gatgccgcgt
     4801 acggcggcgc gctccgaatg tcggagaagt atagagatcg gctgcgcgga atcgagcggg
     4861 cggattctgt gacgctcgat ccgcacaagt ggatgttcgt gccgtttgcg gcgggtgcgc
     4921 tgctcgtgcg cgagggcggg cacgtcttgc gcgatgcgtt cgacatcacg cccgaatatc
     4981 tgagcgagga acgcggcggc caagacgtgc ccttcgactt cttccgctac ggccaactcg
     5041 gcacgcgccg tttcaacgcg ctcaaaatct ggttcgcctt gaagacgctc ggcacggacg
     5101 gctacgcgga gattatcgag cggcagatcg aactcacgcg ctacctcgcc gcccgcatcg
     5161 cggaactcgt tgatttcgag tgtgtcggcg aagtcgagac ggcggtgtgt tgcgtgcgtt
     5221 tcgtgcctgc ggagatgcgc gcggcggaag tcgcggaagt gaacggcgtg cagcaggagt
     5281 tgcagcagcg catcgaacga agcggggagg cgtggctggc aaccaccgtc cttcatggtc
     5341 ggcgcgtgtt gcgcgtcaac gtcaatagct tcctgaccga gcggcggcac atagatgatc
     5401 tggtagaatt acttcgaagc gaaagcacgc agttgtggaa agcgagaagg gctatatgag
     5461 agttatcaga aagatcgtcc tgagtgcgtg ggtgttctgc ctgctgtgcc ctccggctca
     5521 ggcgcaggcg cagccgggcg taaaggccga actgcgttcg cgcgccgcga tcaaatcgcg
//
LOCUS       DASWNS010000228        13593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_424608, whole genome shotgun sequence.
ACCESSION   DASWNS010000228 DASWNS010000000
VERSION     DASWNS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13593
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424608"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5268)
                     /locus_tag="VGB76_07820"
     CDS             complement(<1..5268)
                     /locus_tag="VGB76_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0098846.1"
                     /translation="MSTEISNLSLVEKRALLAQLLKQKADQPEAPSPLSYGQRAWWFL
                     YQLEPQNPAYNIMMPVRILSKVDAAVLRRCFEQLVARHASLRTSYTIDGSKLVQKVQD
                     GAEIDYDTVDASAWDEERLREVVSEEMHRPFDLERGSVLRIRLYTLSEREHVFTMVVH
                     HIAFDLWSLVLFMHELGVVYAAEQDGRPTPLAPLKYRYTDYVRQQLKLLSGAQGEQHW
                     NYWREQLSGELSVLNLPTYAPRPTVQTFRGATSRFTLSTELSDRLKALSQANGATLYM
                     TMLAAYQVLLHRYSGQNDIIVGSLAAAGRNLAEFSGLVGFFDNQIALRADLSRDPTFE
                     QFLAQVRQTVLDALKHQNYPFPLLVEKLQPERDTSRSPIFQTMFILQRAQLPEEAGLI
                     RLGLGMTNARMQLGELILEPMNFDRRVAGGLAGQLDLTLVAADLEDRLECSLQYNPDI
                     FDTDTAEQMARQFQSLLESIVAEPGRSVGTLSLLSDKERQRSIVEWNDTAVEREFECL
                     HRMFERQAERDPDATAAVFRGQRLPYGELNRRANRLAHYLRSLGVGAEVRVGICFERS
                     TEMLVAVLGILKAGGAYVPLDPNYPQDRLAFMLEDARVRVLLTQQHLAERFASCGARI
                     LRLDADWPEIERRDDTNPVLVTTTGHLAYIIYTSGSTGRPKGVMIDHRGAANTIIDMN
                     ERYAVSRDDRVLALSSLSFDLSVYDMLGTLAAGAAIVVPNPSATPDPADWLDLIKREE
                     VTVWNSAPALMEVFAEYVSGHSALMPRSLRIVLMSGDWIPVKLPDQIRSLAANNGIEV
                     HSLGGATEASIWSIAFPIKSVDTTWKSIPYGLPMINQQFHVLDAFLAPTPVGVVGELY
                     IGGIGVARGYHDRQELTAEKFIPDPYAPRPGMRLYRTGDLGRRLPDGNIEFLGRIDGQ
                     VKIHGFRIELGEIETALGNHPSVRETVVKTQVVASGEKQLVAYVVPKQETDDPADPRV
                     AAAATAGMQVWASLVELGREQAKHPTIELDASAFPVLTECLNRLTVAYLCNTFRKLGV
                     FTRPGEELSLEEFMERSGCVARYHKAVKRWLKLLAAQGLLRRRGAAYVSDEPLAIEAF
                     DALWERVYEQLPDTETRASFNYIKSCGENLADVLTGKLHPAQLLFKEGEPTVAESFYD
                     EGFRYCNAVAREATRGIVRAWPRGQTLRVLEVGAGIGSTTAWLLPELNAQTTAYAYTD
                     ISKYFMEMSRKQFGDYPFISYGMLDIEQNPRAQGYEPHSFDFIIASSVLHATRDVKAT
                     LRHVRSLLAPRGLLLLVEETVFHNWFNVVGIQEGFDRFEDEALRQEHPFLTSAQWERE
                     LRAHGFEAVESFTRDRFTAEFLGLDVIVAQASPAMSAHIEREELRSYLRGNLPEFMIP
                     SAYVVLDALPLTPNGKVDHRALPALDRAALDEMERAFVAPRNPTEEVLAGLWAQVLRR
                     EHVGVEDNFFESGGDSLLATQLISRLRNVFNVELPLYCLFEAPTVAGLAAKIDASYGE
                     GRASASPPLRRIPRGGHLPLSYAQQRLWFLDRLVPGNPFYNVTTAVRFTGALDRAVLE
                     RSLNEIVRRHESLRTTFLEVDGQAAQVVGEAWPVNIRLVDFAGRAPETQEEELQSLAT
                     EETQRPFNLIMGPLLRVVLARLGEREHVLLLTLHHIIFDGWSFSVLLREIAALYEAEV
                     TGKPSPLTELPVQYADFAHWQREWLQGGVLEEQLGYWRKQLAGSTVLQLPTDRPRPET
                     QTFRGGRHLSSISAELSASLKELSSRHDVT"
     gene            complement(6123..7067)
                     /locus_tag="VGB76_07825"
     CDS             complement(6123..7067)
                     /locus_tag="VGB76_07825"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098847.1"
                     /translation="MRRNFLAGFTLGILALRLCLGCVFEVQAESASREVVWRDGLVLQ
                     TEPLKSVDAGQAKRSGSQTEDQVFENFEQAFLTPEKVKHLVVKEFDPEMKHLPRQIGT
                     LINLETLEMSCLEKLEELPDEIGKLQKLRALIIDNGNGCAMNISLPRSIGQLENLKVL
                     TLYGALDARDITSEQPLNRAKSKRLPPTFANLRNLEELDLGRNGLSALPVQIAALHKL
                     KRLGLDYNNIRELPAAIGNLKNLRELSLRSNGGVKLPQSLAALKGLRVHMGNNSLKLK
                     DQRQLRSRFPRLVFSFNNEFDDDAANELPARPRPKARR"
     gene            7915..9513
                     /locus_tag="VGB76_07830"
     CDS             7915..9513
                     /locus_tag="VGB76_07830"
                     /inference="COORDINATES: protein motif:HMM:NF037824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0098848.1"
                     /translation="MAQTEKYRLADSTVIEPLINQWAVWADLISPVTYSMHMVHYQIK
                     TLASYVSNPQLHVKACQNPKLAVGPFVNVPEQRAGEVRDLLKSTEQNQSDNIRLAQSL
                     VEFHDYLTKEAKGQSLEPFYDKLPDTLRGYVELLYDYYNHPIVRLLESFLYESPYYKP
                     QLQSMRVFEQKRDNSRPFFLSTPRLLGAEELDLPLRFDSPTIDEIFKLDFEPQPLERI
                     GEMLSLDADGLERLRPLLTQEPVRPHTKYDGDDVRIRYFGHACVLIEWNGVAVLTDPW
                     VGGRSTDGGRERFSFDDLPEKIDFALITHSHHDHFVPETLLRLRHRIECLVVPRTYGL
                     FYTDVSLKLAARKIGFKNVVEMDSLDSIEVPGGEIISVPFLGEHADLAHGKSGYLIRA
                     GHERILFAADSNCLDRRLYEHLRRTVGDIETVFLGMECVGAPLSWLYGALVPAKLQHS
                     LDQSRRTKGSDSTAAFELLNAVGGKRVYIYAMGNEPWLQYGMGLGLTEDSVQVKEANK
                     VLAMARERGFRDAQRPYCKNEIHI"
     gene            9724..>13593
                     /locus_tag="VGB76_07835"
     CDS             9724..>13593
                     /locus_tag="VGB76_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0098849.1"
                     /translation="MKDNNIEDVYRLSPMQEGMLFHHLYSPESDTYFRQQSYTFHGQL
                     NLPAFTQAWQRVIERHAVLRAAFYWQDLEKPLQVINRKVATPLAHHDWRHLPEAEQGE
                     RLKALLAAEQSLGFDLTRAPLMRIVLVQVADEVSHLVWSYHHLLMDGWSKYQVLREVV
                     SLYDAFCGGKDLQLGRSRPYRDYISWLKQQDASGAEAFWRESLKGFDAPTPLGAGASN
                     HHGLNAGDVYDAREILLPTDTTAALKSLARQHQVTLNTLVQGAWALLLSRYSGEEDVL
                     FGITVSGRPASLPGVENMVGLFINTLPLRVKVPPAAELLPWLKKLQAQQAELSQFEHS
                     HLSDIQGWSEVARGQMLFESILVFDNFPVDHSLRAEGQLRFKPLASVERNNYPLTLVA
                     FADERLMLRIPYDTQRFESAAIERMVGHLQSLLEGMIARPQQRLGEVPLLSHEEQRRL
                     LSELNNTGHDISSNLTVHQLFEAQVERTPDAVALVLGQASLSYRQLNERANQLAHYLR
                     GLGVRPEVRVGICVERSLEMLLGLLGTLKAGGVYVPLDPGYPQERLSFLLEDLQVPVL
                     LTQDHLLFELPTYAVASELVCLDSDWSAIATSSTDNPESLLTGENLAYIIYTSGSTGI
                     PKGVCLSHRVAANHFLAAQSEFRLDSTDVVLEFASFNFDVSLEQLFVPLLAGATVVVR
                     GAQPWGREDFFRHVADFGLTVVNFPPAYWTQVVPAPGTDLAARLAAQLKLVIIGGDQM
                     PAHTARLWQQSGLRDVRLLNAYGPTETAITATTFTVPDLTDEDAATLQMPIGRPLAHR
                     AVYILDRQGAPVPVGVTGELHIGGALLARGYLNLPALTAERFIPDPFSGQAGARMYRT
                     GDQATCSADGNIRFLGRVDNQVKIRGYRIELGEIEAALYACEGVRDAAVIVSERVAGE
                     KQLIGYVVMQAGTTVGTGELKERLRERLPDYMVPNTFVVLEEMPLTPNGKVDRRALPA
                     PDETRGEAVDSYQAPRTPVEEMVANIFASVLRVARVGVEDNFFELGGHSLLATQVISR
                     IRESLDVELPVRAVFEHPTAAGLATQVEAESHAGYEAPPLGKREDAGAAPLSFAQQRL
                     WFLDNFEPHSNAYNMPVALRLKGLLDVGALERTLTEVCRRHEVLRTRFAVRDGAPVQV
                     IDAPTPFNLIVTEVAQEAQEQRVRQLIAAEAARPFDLAAGPLVRAQLLRLVTEEHVLL
                     FTMHHIISDGWSIGVLIKEVATLYNAYVQGQPSPLAELPIQYADYAVWQREWLQGEAL
                     ERQLDYWRQQLAEAPAALELPTERARPA"
BASE COUNT         2702 a         3947 c         4166 g         2778 t
ORIGIN      
        1 ggttacgtcg tggcggctgc tcaactcctt gagggaggct gacagttcgg cggaaatgct
       61 actgaggtgg cgtccgccgc ggaaggtctg cgtctccgga cgcgggcggt cggtcggcag
      121 ttgcaggacg gtgcttccgg caagttgctt gcgccagtag ccgagttgtt cttcgagcac
      181 gccgccctga agccactcgc gctgccagtg tgcgaagtcg gcatactgca cgggcagttc
      241 cgtgagcggc gagggttttc cggtgacctc ggcttcgtag agcgcggcga tctcgcgcag
      301 cagcacgctg aaagaccagc catcgaagat gatgtggtgc agcgtgagca gcagcacgtg
      361 ttccctctcg ccgaggcgcg cgagcacgac gcgcaacagc ggccccatga tgagattgaa
      421 ggggcgttgc gtctcctctg tggcgaggct ttgaagttct tcctcctgcg tctcgggcgc
      481 acgcccggcg aagtccacga gcctgatgtt aacaggccac gcctcgccga cgacctgcgc
      541 cgcctggccg tcaacttcga ggaaagtcgt gcgcagcgat tcgtgccgcc tgacgatctc
      601 gttgaggctt cgttccagca ccgcgcggtc gagcgcgccg gtgaagcgca cggcggtggt
      661 gacgttgtag aaggggttgc ccggcacgag ccggtcgaga aaccagaggc gttgctgcgc
      721 gtaggagagc gggaggtggc cgccgcgcgg gatgcgtcgc aacggcggag atgcgctcgc
      781 gcgcccctcg ccgtaagacg catctatttt cgcggcgagt ccggcgacgg tcggcgcttc
      841 aaacaggcag tacaacggta gctccacgtt gaagacgttg cgcaggcgcg agatcaactg
      901 tgtggcgagc agcgagtcgc cgcccgactc gaagaagttg tcttcgacgc cgacgtgctc
      961 gcggcgcagc acttgtgccc agagtccggc cagcacctct tctgtcggat tgcgcggcgc
     1021 gacgaaagcg cgctccatct catcgagcgc agcccggtcg agcgcgggca gggcgcggtg
     1081 gtcaaccttt ccattcggcg tcaggggcag ggcgtcgaga acgacgtagg ccgaagggat
     1141 catgaactcc ggcaggttgc ctctcaggta actgcgcagt tcctctctct cgatgtgcgc
     1201 ggacatggcc ggggatgcct gcgcgacgat cacgtccaga cccaggaatt cggcagtgaa
     1261 gcggtcgcgg gtgaaggact cgacggcctc gaagccgtgc gcgcgcagtt cgcgctccca
     1321 ctgcgcggag gtcaggaagg gatgctcctg gcgcagcgct tcgtcctcga aacggtcaaa
     1381 gccctcctgt atgccgacca cgttgaacca gttgtggaag accgtctcct cgacgagcag
     1441 cagcagtccg cgcggggcga gcagggagcg aacgtggcgc agcgtcgcct tcacatcgcg
     1501 cgtcgcgtgc aggacgctcg acgcgatgat gaagtcgaag gagtgcggct cgtagccctg
     1561 tgcgcgcggg ttctgttcga tgtcgagcat tccgtagctg atgaacgggt agtcgccgaa
     1621 ctgcttccgg ctcatctcca tgaagtattt ggagatatcc gtgtaggcgt aggcggtggt
     1681 ctgcgcgttg agttcgggca gcagccacgc cgtcgtcgag ccgatgcctg cgccgacttc
     1741 cagcacacgc agggtttgac cgcgaggcca cgcgcggacg atgcccctcg tcgcttcgcg
     1801 ggcgacggcg ttgcagtagc ggaagccttc atcgtagaag ctctccgcca cggtcggctc
     1861 gccctctttg aagagaagtt gcgcgggatg aagtttgccg gtcaacacgt cggcgaggtt
     1921 ctcgccgcat gacttgatgt agttgaaaga ggcgcgtgtc tccgtgtcgg ggagctgttc
     1981 gtagacgcgt tcccagagcg cgtcgaacgc ttcgattgcg agcggctcgt cgctgacgta
     2041 agccgcgccg cgtcgccgca gcagcccttg cgcggcgagc agtttcaacc aacgcttcac
     2101 ggccttgtga taacgcgcca cgcagccgct acgctccatg aactcttcga gcgacaactc
     2161 ctcgccgggg cgcgtgaaga cgccgagctt gcggaaagtg ttgcagagat aggcgaccgt
     2221 caggcggttc aggcattcgg tcaagaccgg gaaggccgac gcgtcgagtt cgatggtcgg
     2281 gtgtttggcc tgctcccgac ccaactccac cagcgacgcc cagacctgca tgcctgctgt
     2341 cgcagcggcg gcgacacgcg ggtcagccgg atcgtccgtc tcctgcttcg gcacgacgta
     2401 ggccacgagt tgcttctcgc cggaggcgac aacctgcgtc ttcacgaccg tctcccggac
     2461 ggacggatga ttgccgagcg ccgtctcaat ctcgcccaac tcgatgcgga agccgtgtat
     2521 cttcacctgc ccgtcgatgc gaccgaggaa ttcgatgttg ccgtcgggca ggcggcgtcc
     2581 gagatcaccc gtgcgataga ggcgcatccc aggccgggga gcgtacgggt cggggatgaa
     2641 cttctccgcc gtcaactcct gccggtcgtg gtagccgcgc gcgacgccga taccgccgat
     2701 gtagagttcg cccaccacgc cgacgggcgt gggtgcgagg aacgcatcga ggacgtggaa
     2761 ctgctgatta atcatcggca gcccgtaggg aatactcttc catgtggtgt ccacgctctt
     2821 gatggggaag gcgattgacc agatggaggc ttccgtcgca ccgccgaggc tgtgcacctc
     2881 gatgccgttg ttggccgcaa gcgacctgat ctgatcgggc agttttacgg gaatccagtc
     2941 tccgctcatc agcacgatgc gcagcgagcg cggcatcagg gctgagtgtc cggagacgta
     3001 ttcggcgaac acctccatca gcgcgggcgc ggaattccag accgtcacct cttcgcgctt
     3061 gatcaggtcg agccagtcgg cggggtcggg cgtggcggaa ggattcggga cgacaattgc
     3121 cgccccggcg gccagcgtgc cgagcatgtc gtagaccgag aggtcgaagc tgagggagga
     3181 gagggcgagc acacggtcgt cgcggctcac ggcgtacctc tcgttcatgt cgatgatggt
     3241 gttggccgcg ccgcgatggt cgatcatcac gcccttcggg cgacccgtgg agccggacgt
     3301 gtagatgatg taagcgagat ggccggtcgt cgtgaccagg acagggttcg tgtcgtcacg
     3361 gcgctcgatc tcaggccagt cggcatccag acgcaggatg cgcgcgccgc acgaggcgaa
     3421 cctctctgcc agatgctgtt gcgtgagcag cacgcgcacg cgcgcgtctt cgagcatgaa
     3481 ggcgaggcga tcctgcgggt agttcgggtc gagcgggacg taagcgccgc ccgccttgag
     3541 gatgccgagg acggccacca gcatctccgt cgaacgctcg aagcagatgc ccacccgaac
     3601 ctccgcgccg acgccgagcg aacgcagata atgagccaaa cggtttgcgc gccggttcaa
     3661 ctcgccgtac ggcaggcgtt gaccgcgaaa gacggcggca gtcgcgtccg ggtcgcgctc
     3721 ggcctgccgc tcgaacatcc ggtgcaggca ctcgaactct cgctcgaccg ccgtatcgtt
     3781 ccattcgacg attgagcgtt gccgctcctt gtcgctcagc agcgagagcg tccccacgct
     3841 ccggcctggt tcggcgacga ttgattccag aaggctttga aactgtcgtg ccatctgctc
     3901 agccgtgtcg gtgtcgaaga tgtcggggtt atattgcaga gaacattcga gcctgtcttc
     3961 gaggtcggcg gcgaccagtg ttagatcaag ctgtccggcc agaccaccgg cgacgcggcg
     4021 gtcgaagttc atcggttcca gtatcaactc gccgagctgc atgcgcgcgt tcgtcatgcc
     4081 cagacctaac ctgatgagtc cggcctcctc cggcagttgc gcgcgttgga ggatgaacat
     4141 cgtctggaag atcggggagc ggctggtgtc gcgctccggt tgcagctttt cgaccagcag
     4201 ggggaacggg tagttctggt gcttcagagc atcgagcacg gtctgtcgca cctgcgcgag
     4261 gaactgctcg aaggtcgggt cgcgggagag gtctgcgcgc agcgcgatct ggttgtcgaa
     4321 gaagccgacc agcccggaga attccgcgag gttcctgccc gccgcggcaa gcgagccgac
     4381 gatgatatcg ttctggccgc tgtaacggtg gagcaacacc tgataggcgg ccagcatggt
     4441 catgtagagc gtcgcgccgt tggcctgact gagcgccttc aggcgatcag acaactcggt
     4501 gctcagagtg aagcgggagg ttgccccgcg aaatgtctgg acggtcgggc gcggcgcgta
     4561 ggtgggcaag ttgagcacgg acaactcgcc cgaaagctgc tcgcgccagt aattccagtg
     4621 ctgctcgccc tgcgcgccgg aaagtagttt caactgctgg cggacgtaat ccgtgtagcg
     4681 atatttgagc ggcgcgagcg gcgtgggacg gccatcctgc tccgccgcgt agaccacgcc
     4741 caactcgtgc atgaacagca cgagcgacca gaggtcgaag gcgatgtggt gcacgaccat
     4801 cgtgaagacg tgctcgcgct cggacagcgt gtagagcctg atgcggagca ccgacccgcg
     4861 ctcaaggtcg aacgggcggt gcatctcttc ggaaaccacc tctcgcagac gctcctcatc
     4921 ccacgccgac gcgtccacgg tgtcgtagtc gatctccgcc ccgtcctgaa ctttctggac
     4981 cagcttgctc ccgtcaatcg tgtagctcgt tcgcagcgag gcgtggcgcg caaccaactg
     5041 ctcgaagcag cggcgcagca ccgcagcatc caccttcgag aggatgcgca cgggcatcat
     5101 gatgttatag gcggggtttt gcggctccag ttgatagagg aaccaccacg cgcgctggcc
     5161 gtaggagagc ggcgacggcg cttcgggctg gtcggctttc tgtttgagca gttgcgccag
     5221 cagggcgcgc ttttctacca gagagaggtt ggagatttcc gtagacataa tcgtttcaac
     5281 cattcttatc gatgagcacg gaagggggta atcgattgac gagtcgggcg gtactcgtcc
     5341 cctgcttgat gccgaccggc ggcagtttca actgacgttc gttagcaacg aattacggag
     5401 ttcaatcgcc atcagcgaat tgaggccgag gatgttgagc ggcctctcaa ggccgaacgc
     5461 aaaagggcgt gtgatgtgca ggactccgga gtcgaacccg cgcacgcagg cgtcaagcct
     5521 cccacgttgc tactccggcg gcgggaataa caacagcgcg cgagtgagtt ccgccccgtc
     5581 tccatccgag tttgcgaggc ggcttggtcc tgatagtggg tcatggtcaa cacgttgaac
     5641 gagaaagggt ctttaatcct gtgaatctcc agatgcggtg aaaggagatt ttcatcggca
     5701 aacgttgcag gtggaagctt tcgcggtgaa agagaatcgc ctgattggcc ggatacggag
     5761 tagagtcgca gatttatgcc gacgacgaca gggaaaactg actacgagaa gaattcggaa
     5821 ttcttacctc aattctaaaa atgactggta attataaaaa ttaagatgga gccaacaggt
     5881 gaatctaatc tccgcgacga gaagtagagc acgcacatta ctatcagagc agaatggctg
     5941 tcaagcgtcg ttgcacaaat attgacaggc agagcttaat ttttatgcat caacgagttc
     6001 tgctcggaaa tccgggtgtg tggcaggaaa tatcttggcc gccgttagtt gagcgggaga
     6061 gtctgctacc gcctcgatct cctgtgcggg cggcaatttt ttggcactct aaaacgtttt
     6121 cgttaccgtc gggctttcgg tcttggtctg gccggtaact catttgcggc gtcgtcatca
     6181 aattcattat taaaagagaa aacaaggcgg ggaaaacggc tgcgtagctg tctctggtct
     6241 ttcagtttga gagagttatt tcccatgtgc actctcagcc ccttgagagc ggccagcgac
     6301 tgcggcaact ttacgccgcc gtttgaacgc agggagagtt cccttagatt ctttagatta
     6361 ccgatggcag cggggagttc acgaatgttg ttatagtcga gtcccaatct ttttaattta
     6421 tgaagggcgg cgatttggac gggcaacgcg gaaaggccat ttctgcctaa gtccaattct
     6481 tccagattcc gcaggtttgc aaaggttggc ggtaatctct tgcttttggc gcggttgaga
     6541 ggttgctccg aagtgatgtc tcgcgcatcg agcgcgccat ataatgtcaa cactttcaga
     6601 ttttctaatt gaccgatgga gcgcgggaga gagatgttca tcgcgcagcc gttaccgttg
     6661 tcgatgatga gcgctcttaa cttttgtaac tttccgattt cgtcgggtag ctcttccaac
     6721 ttctccagac aagacatttc caacgtttcc aaattaatca acgtccctat ttgacggggc
     6781 aaatgtttca tctcaggatc aaattctttg accacgaggt gcttcacttt ttccggcgtt
     6841 aagaaagcct gctcgaaatt ctcgaacacc tgatcctccg tctggctgcc gctgcgcttc
     6901 gcctggcccg catccacgct ctttaaaggc tcggtctgca acacaagccc atctcgccac
     6961 acgacttccc tcgacgccga ttccgcttgc acctcgaaga cgcagccgag gcagaggcga
     7021 agtgcaagga ttccgagtgt aaaaccggct aagaagtttc ttcgcatgat ctttttctcc
     7081 tgtgaaacct ggaagactga agtgtctgat atacgcctga gatcagccct gcccggtgaa
     7141 cccgttcccg ttagtttctc cctggcactt ccggcctgaa ctaacgttta cgcaagggtg
     7201 gaagtagtaa tttttgggca gaactcgttg atgcatagaa attaagccct acctgtcaat
     7261 atttgtgcaa cgacgcttga cagccattct gctctgatag tactctgcgt gctctacttc
     7321 tcgtcgcgga gattagattc acccgttggc tccatcttaa tttttataac taccagtcat
     7381 ttttaggttc aagacaagaa ttcataattc ttctcgtagt cagttttccc tgtcaccatc
     7441 tgtacgtttg tctatccccg gtaaaacgtt tgaatgaacg ggaagtatct atccatttat
     7501 ggcaccgttc gttgcgaaat agatagtctt gcgccgcaag tgtttcaggt gaggacggaa
     7561 gttctgattt tgccgctcaa tcgctgcctc catgctctgc ccctgcggtg agggttctct
     7621 ggaatcggct ttagatagga gtttcctgcc aacggcaaaa gctacggctc aatatcttat
     7681 ccggccaatc aagcgattct ctttacataa cgaaagcccc catccgcgca ctattggttg
     7741 atggaaatct tctttcactg cgtgcggcct gttaagcagt gctgacctcc gaagacggaa
     7801 aatcgtcgtt gcgatgggcg agatgtcggc gagaaagagc tttaaactgt cgttcaatgt
     7861 attccccaac ctgtggctct atcttccagc gaaattattt aagagataaa tccaatggca
     7921 caaaccgaaa aataccggct cgccgattcg actgtgattg agccgctgat caaccagtgg
     7981 gcggtgtggg cagacctcat ctctcccgtg acatacagta tgcacatggt gcattaccag
     8041 attaaaactc tcgcctctta tgtctctaac ccgcagcttc acgtgaaggc gtgccagaac
     8101 ccgaaacttg ccgtcggtcc cttcgttaat gtgcccgagc agcgcgcggg agaggtgcgc
     8161 gacctgctca aaagtaccga acaaaaccag agcgataaca tccggttggc tcaatctctc
     8221 gtcgagtttc atgactattt gacgaaggag gcgaaggggc agagccttga gcctttctac
     8281 gacaaactgc cggacacgct gcgaggctac gtcgaacttc tatacgatta ttacaatcac
     8341 ccgattgtca ggctgctgga aagctttctc tatgaaagcc cttactacaa gccgcaactc
     8401 cagtcgatga gggtttttga gcagaagcga gacaattcgc gcccgttctt cctgagcacc
     8461 ccgcgcctcc tcggcgcaga ggagttggac ctgcccctgc gtttcgacag cccgacaatc
     8521 gacgagatct tcaaactcga tttcgagccg cagccgttgg agcgcatcgg cgagatgctc
     8581 tcgctggatg ccgacgggct ggagcgtctg cgtccgctgc tgactcagga gcctgtgcgt
     8641 ccccacacga aatatgacgg cgacgatgta cgcatccgtt atttcggtca tgcctgtgtc
     8701 ttgatcgaat ggaacggggt ggcggtgttg actgatccgt gggtgggagg cagatcgacg
     8761 gacggcggac gggaacgctt ctcttttgac gacctgccgg aaaagataga tttcgccctg
     8821 atcacgcata gccatcacga ccactttgtt cccgaaacgc tgctacgcct tcgccatcgc
     8881 atcgaatgtc tcgtcgtgcc gcgcacctac gggcttttct acacggatgt ctcgctgaaa
     8941 ctggccgcca gaaaaatcgg gttcaagaac gtcgtggaaa tggactcgct cgattccatc
     9001 gaagttcccg gcggagaaat tatctccgtc ccgtttttgg gcgagcacgc agacctcgca
     9061 cacggaaaga gcggctacct gattcgcgcg gggcatgagc gcatcttgtt cgcggcggac
     9121 tcgaactgtc tggatagacg cctctacgag catttgcggc gcacggttgg cgacatcgag
     9181 acggtctttc tgggcatgga gtgtgtcggc gcgcctctgt cctggttata tggcgcgttg
     9241 gttccggcga agctccagca cagtctcgat cagagcagga gaacgaaggg cagcgattcg
     9301 accgccgcct tcgaactcct gaacgccgtc ggcggcaaac gcgtctacat ctatgcgatg
     9361 ggaaatgagc cgtggctcca gtacggcatg ggcttggggc tgacggaaga ttccgtgcag
     9421 gtcaaagagg ccaacaaggt gctcgccatg gcgcgcgaga gaggctttcg ggatgcgcag
     9481 cggccttact gtaaaaacga gattcacatc tagtcgcaac aaccctccgt ctagcccgga
     9541 tatatcaaga ctaaatagat gacatctttt tagctatcgt tttaagtccc tgcgcattcg
     9601 cctgagcggg aatgtttttc ggcaataccc ctgccgacgc tcctgcaagc gtccaccagc
     9661 cggagagaat cggacgacat actttcgcag agcgtccaaa tcagcggata ggaaataatt
     9721 ctgatgaaag ataataatat cgaggatgtc tacaggctct cgccgatgca ggaggggatg
     9781 ctctttcacc atctgtacag ccccgaatcc gacacctact ttcgtcagca aagctatacc
     9841 ttccacggac aactgaacct tccggccttc acgcaggcat ggcagcgcgt tatcgagaga
     9901 cacgccgtgc tgcgtgccgc tttctactgg caagacctgg aaaaaccgct gcaagtgatc
     9961 aaccgcaagg tggcgactcc gcttgcgcat cacgactggc ggcaccttcc ggaagccgaa
    10021 caaggcgaac gtttaaaagc actgctggcg gcggaacaga gcctcggttt cgacctcacg
    10081 cgggcgccgc tcatgcgcat cgtgctcgtg caggtggctg acgaagtctc tcatctcgtc
    10141 tggagctatc atcatctatt gatggatggg tggtctaagt atcaggtgct cagggaagtg
    10201 gtgtcgctct acgatgcttt ttgcggaggg aaggatttac aacttggccg ctcacgcccg
    10261 tacagagatt acatctcttg gttgaagcaa caggacgcgt ccggcgcgga ggcgttctgg
    10321 cgggagtctc tgaaaggttt cgacgcgccg accccgctcg gcgcgggcgc gtcgaaccac
    10381 cacggactaa acgccggaga cgtatacgac gcgcgtgaga ttctcttacc gaccgacacc
    10441 accgccgcct taaagtcgct ggcgcggcag catcaagtca ccctcaacac actggtgcag
    10501 ggggcgtggg ctttactcct gagccgctac agcggcgagg aagacgttct gttcggcatc
    10561 acggtttccg gccgcccggc ctcgctgccg ggggtcgaaa acatggtcgg cctctttatc
    10621 aacaccctgc cgctgcgcgt caaagttcca ccggcggcgg aactcctgcc gtggctcaag
    10681 aaacttcaag cgcagcaggc ggagcttagt cagttcgaac acagccacct ctcggatata
    10741 caaggctgga gcgaagtggc gcgcgggcaa atgctgttcg agagcatcct cgtgtttgac
    10801 aacttcccgg tggaccactc tctccgcgcg gaggggcaac taaggttcaa acctctggcg
    10861 tcggtggaaa ggaataacta cccgctgacc ctcgttgcct tcgcggacga gcggttgatg
    10921 ttgcgcattc cctacgacac gcaacgcttc gagtcagccg ccatcgaacg catggtcggc
    10981 cacctgcaaa gcctgcttga gggcatgatc gcccgtccgc agcagcgtct cggcgaagtg
    11041 ccgctcctgt cgcatgaaga acaacgacgc ctgttgtcgg agttgaacaa caccggccac
    11101 gatatctcat cgaacctgac ggttcaccaa ctcttcgaag cgcaggtcga gcgaacaccg
    11161 gacgccgtgg cactcgtgct cggtcaggcg agcttgagtt atcgccaact aaacgagcgt
    11221 gccaatcaac tcgcgcatta tttgcgtggt cttggtgtca ggccggaagt gcgcgtaggc
    11281 atctgcgtgg aacgctcgct ggagatgctg ctcggcctgc tcggaacact caaagccggc
    11341 ggcgtctacg tgccgctcga ccccggctat ccgcaggagc gactctcttt cctgctggaa
    11401 gaccttcaag ttccagtgct cctgacgcag gaccacctgc tcttcgaatt gccgacttac
    11461 gcggtagcgt cggaattggt ctgtctcgat tcggattgga gcgccatcgc tacgagttcg
    11521 acggataatc ccgaaagcct cctgacgggc gaaaacctcg cgtatatcat ttacacctca
    11581 ggctccaccg gtatccccaa aggcgtctgt ctctcgcacc gcgtcgccgc caaccacttt
    11641 ctcgccgccc aaagcgagtt tcgcctcgac tcaaccgatg tcgtgcttga gttcgcctct
    11701 ttcaacttcg atgtctcgct tgagcaactc ttcgtgccgc tactagccgg agccaccgtc
    11761 gtcgtgcgcg gcgcgcagcc ctggggtcgt gaagacttct tccgtcacgt cgcagacttc
    11821 ggcctcaccg tcgttaactt ccctcccgcc tactggactc aagtagtgcc cgcgcccggc
    11881 accgacctcg ccgcccggct ggccgcacag ttgaaactgg tgatcatcgg cggcgaccag
    11941 atgcctgcgc acacggcgcg tttgtggcaa cagtccggcc tgcgcgatgt gcgcctgttg
    12001 aacgcctacg ggccgaccga gacggccatc accgcgacga ccttcaccgt gcccgacctg
    12061 acggacgagg acgcggccac tttgcagatg ccaatcgggc gtccgctggc gcaccgtgcc
    12121 gtctacatat tagaccggca gggagcaccc gtgccggtcg gcgtgacggg ggaattgcac
    12181 atcggcgggg cgctgctggc gcgcggctat ctgaatcttc cggcgctgac ggcggagcgg
    12241 ttcatccccg atccgtttag cgggcaagcg ggcgcgagga tgtatcgcac gggggatcag
    12301 gcgacgtgtt cagcggacgg gaatatacgg ttcctcggac gggttgataa tcaagtaaag
    12361 attcgcgggt atcggatcga gttgggagag atcgaagcgg cgctgtacgc ctgcgaggga
    12421 gtgcgagatg ctgcggtcat cgtcagcgag cgggtggccg gggagaagca gttgatcggc
    12481 tacgtagtga tgcaggcggg tacgacggtg ggaacgggcg agttgaagga gcgtctgcgg
    12541 gagcgtctgc cggactacat ggtcccgaac accttcgtag tgttggagga gatgccgctg
    12601 acgccgaacg gcaaggtaga ccgccgcgct ctgcccgcgc ccgatgagac gcggggggaa
    12661 gcggttgaca gttatcaagc gccgcgtacg ccggtcgaag agatggtggc gaacatcttc
    12721 gcgagcgtgc tgcgggtcgc cagagtggga gttgaagata acttctttga actcggcggc
    12781 cactcgctgc tcgccacgca ggtcatctcc agaatcagag agagtcttga cgtggaacta
    12841 cctgtgcgcg ccgttttcga gcatccgacg gcggcgggct tagccacgca ggtcgaggcg
    12901 gaaagccatg ccggatacga agcgccgccg ctcggaaagc gcgaggacgc cggagccgcg
    12961 ccgctctcgt ttgcgcagca acgtctatgg tttctcgata acttcgagcc acacagcaat
    13021 gcgtacaaca tgccggtcgc cctgcgactg aaagggcttc tcgacgtggg agcgttggaa
    13081 cgcacgctga ccgaagtgtg ccggcgacac gaggtgttgc gcacaaggtt tgccgtccgg
    13141 gatggcgcac cggtgcaagt catcgacgcg ccgacgccct ttaacctgat cgtgaccgag
    13201 gtggcgcaag aggcacagga gcaacgggtc agacaattga tcgcagcgga agcggctcgt
    13261 cccttcgacc tggcggcggg accactggtg cgcgcccagt tgttgagact ggtgacggag
    13321 gagcacgtgc tgctgttcac gatgcaccac atcatcagcg atggttggtc aataggcgtt
    13381 ctcattaagg aagtcgccac cctctacaac gcttacgtgc aagggcagcc gtcgccgctc
    13441 gccgagttgc cgattcagta cgccgattat gccgtctggc agcgagagtg gttgcagggt
    13501 gaggcgttgg agcggcagtt ggactactgg cggcagcagt tagcagaagc acccgccgca
    13561 ctggaactgc ctaccgagcg agcacgcccc gcc
//
LOCUS       DASWNS010000229        14925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_192490, whole genome shotgun sequence.
ACCESSION   DASWNS010000229 DASWNS010000000
VERSION     DASWNS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192490"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VGB76_07840"
     CDS             complement(<1..197)
                     /locus_tag="VGB76_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098850.1"
                     /translation="MVSLGRCDALREVQLAMLSGTQKVSGGQQRGLSGEQISGGRRES
                     EPPLLLGGLHPVGSMAGHVSS"
     gene            complement(191..307)
                     /locus_tag="VGB76_07845"
     CDS             complement(191..307)
                     /locus_tag="VGB76_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0098851.1"
                     /translation="MLSACETGVGEVQNGDDVYGLRRALVLAGSESQVMTLW"
     gene            complement(371..4237)
                     /locus_tag="VGB76_07850"
     CDS             complement(371..4237)
                     /locus_tag="VGB76_07850"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEY0098852.1"
                     /translation="MSAQQNQTDYSQFIAENFGANATYVETLLQRFRSDPALVDESWR
                     TYFMEMLGETAAPASTSGNGATNAAAAPRPADGNTATAAAATSARTAETAAPVAVPAQ
                     PPAATPEPATSQPQSAPAQTAAAAAAAPAQPGASTAETSPIRGAALKIVENMETSLSV
                     PTATSNRQVPVKLLEENRAIINKHLKEQSGGKASYTHVIAYALLRALDKYPQLNDGFT
                     LAEGKPARVRRAGVNLGVAIDIQKKDGTRTLLVPNIKAANSLSFSQFIRAYDDVVKRA
                     RDGKLGLPDFQETTISLTNPGTIGTVASTPRLMAGQSVIIATGAIEYPAEYHAMSPEA
                     LSQLGISKTLTISSTYDHRIIQGAESGAFLAHVHELLVGKHDFYENIFLDLGIAYRPM
                     QWAVDRNPALLGGDRTQEQIVKQARVLELINAYRVRGHLIADIDPLHAMPVHHHAELD
                     LETYGLTIWDLDRTFITGGLGGHESSTLREILDILRRAYCGKVGTEYRHIQSKEQKTW
                     IRERIRQEFVYPEPLPVETRKQLLDRLIAAEQFERFLHTKYLGAKRFSLEGCETIIPL
                     LHQLIEKAAEHGVEEITLGMAHRGRLNVLANVVGNFSERIFTAFEGSVHPNFPADEGD
                     VKYHQGARGRRETASGREVAITVSPNPSHLEFVDAVVEGMVRARQDAMNAEREEVIDR
                     ALPVLLHGDAAFAGQGIVMETLNLAELRGYRTGGTIHIIINNQIGFTTSPEQGRSTIY
                     STDVARMTQLPIFHINGDDPETAFRVLRIALDYRQEFNKDVVLDVIGFRRLGHNEGDE
                     PSYTQPLMYARVKAHPGVRAIYAGRLIREGVLTEADVEAMIKARVEHYEGAQARAKEI
                     VAGKPKMSEVPAPVVEEDGSEVVETGVSADVVREIGQRIAIVPEGFHVNPKMVSQLAR
                     REKMGAGQTPLDWAFGEAVAFGSLALEGTNVRLSGQDSGRGTFSQRHAILYDTQTGRA
                     WTPLSELAARENGRPRFEVFDSSLSEQGVMGFEYGYSVEALDALVMWEAQFGDFSNGA
                     QVIIDQFIAPGVDKWQQPSRLALLLPHGYEGQGPEHSNARLERYLQLCAENNMQVCYP
                     TTPAQYFHLLRRQVKQEIARPLVVMTPKSLLRLPAATSSVAELTTGGFLPVIDDAEIE
                     NRAEVLRIVLCSGKVYYDLNAARLKSDDRRVAIVRLEQFYPFPERLLREIFARYPNAA
                     QLVWAQEEPKNYGGWHFVEPRLMGMMKNCERPYYVGRTASASPATGSYTIHELEQREL
                     VNNALTTDAPVISNASTDKYAGEADS"
     gene            4421..5197
                     /locus_tag="VGB76_07855"
     CDS             4421..5197
                     /locus_tag="VGB76_07855"
                     /inference="COORDINATES: protein motif:HMM:NF018626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HEY0098853.1"
                     /translation="MENAPRVELMPSSKFFTHATFLCVLLFACGACAIPRAVSQRKGE
                     TPASRAATPTPQAAVKPVYESPVLERLVAAAVERTTHEVEYDPTYYKLEYPNGDVPAD
                     KGVCTDEVIRSYRALGIDLQKEVHEDMSANFSRYPTKFGLTTTDTNIDHRRVPNLRVF
                     FERKGRSLPITDEPADYKPGDIVTWDLNASQTHIGIVVDVPSATPGRYMIMHNIGQGP
                     KIEDILFAWKITGHYRYTGTTAPPPAKPAPRPSASRRSTR"
     gene            5373..7124
                     /locus_tag="VGB76_07860"
     CDS             5373..7124
                     /locus_tag="VGB76_07860"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0098854.1"
                     /translation="MSYSRTITHTSPDSKQRRPRPDSPRPKNARLSWIALCFVAALLL
                     SSCERASVNEPEQRPSKDVSKTENKAPLVSAPAMPNPELEMARETATGLYAQRRDGEV
                     DNKQFNTESYSHIDENPFFEVARAPLSTFSIDVDTASYSNTRRFLRDGQLPPKDAVRI
                     EELINYFPYDYPQPRGDAPFSINAEVSSCPWNTKRRLVHIGLQGKRISKEELPPANLV
                     FLLDVSGSMNASNKLPLVKSSLRMLAEQLDARDRISIVVYAGSSGLVLPATPGDRRGE
                     IIAALDALDAGGSTNGGEGIQLAYKVASDNFIRGGINRVILATDGDFNVGVTSEGELV
                     RLIEEKRKSGVFLSVLGFGTGNVKDATMEKLSDKGNGNYAYIDDVQEARKVLGEQLGA
                     TLATIAKDVKIQVEFNPRQATAYRLIGYENRLLRDQDFNDDLKDAGEIGAGHTVTALY
                     EVVPFGQKFENPGIDELKYQTPVESPARADSRELMTIKIRYKDPEESASKLLSVWVSD
                     AGGTLESASNNLKFSSAVAGFGMLLRDSKYKGDVRYADVARLARAAAGADTQGYRTEF
                     IQLVERAQTLSRQSARN"
     gene            7313..9436
                     /locus_tag="VGB76_07865"
     CDS             7313..9436
                     /locus_tag="VGB76_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0098855.1"
                     /translation="MNEPLLREFLAEADERIELLFAEIERLRRVHAEADGRARRELIA
                     RIFRHVHTLKGSAATLPQLAAVSRLAHEFENLLDAIRAGRAPLTDESIDACEDATDAL
                     SLALASHARGDEDAAEPPPAIFQLLRQHARGAPQLSIAQEANPRTGSVAALPPEITRA
                     LSGVEQRHLSESVKEGGRALLVAASFDLATLDEQFGRFNAALGACGEIISTFPGDPAS
                     APDQISFRVLYTTTCTRVELEERLAPFEAKLDWREDAPEPDGASANGAEASTFDTDAP
                     PEASTDSTPDPSSVAPAATATRAPIFVRVPLHELDELVTATHELFTETLAALEQTPAV
                     PETVAAVAAAETTHAAGATGAERARRLRENFSALEERVMSLRMQPLMPVLERAQRAAR
                     IAARAAGKEIEFEVAGGDVRVDRALAEQIGEPLLHLLRNAVDHGIETAEERRATGKPL
                     RGRVRLEVAMEGSRVRVRVADDGRGIDTERVARVAVACGLLEAGAHVTDEQALRLIFR
                     PGFSTAAEVSAMSGRGVGLDVVEHALERAGGELRVRSARGAGTTFELRVPLTLALVAV
                     FVVRARGYAYAFDASHIVATAYLEPSEIEGVETGRGSVLWEGERLPFVSLRSLLGEAW
                     TPEEGAGERLPVVVVRRERDGSWQSASAPESFVIAVDRIEGEREPLVRSLGRHATRWR
                     GVSGAFTERDGTVALLLDLPRLLEK"
     gene            complement(9464..9808)
                     /locus_tag="VGB76_07870"
     CDS             complement(9464..9808)
                     /locus_tag="VGB76_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098856.1"
                     /translation="MLRTIHTACTYLIIALGVLHLLFTFHDYDAFTLGALWFAGAGFA
                     LMFAGFLNLVALRHAAGDGLVRALCVVANLICASLFGAALWLMQQPQVFVGVALFTVA
                     AIASLAGRTRAS"
     gene            complement(9922..11352)
                     /locus_tag="VGB76_07875"
     CDS             complement(9922..11352)
                     /locus_tag="VGB76_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0098857.1"
                     /translation="MLTEDTFFSSVVELAARLRARKLSPVELTEGYLARLEQFGARLG
                     AVATIMRRSALNEARQAESEIRAGRYRGLLHGIPYGAKDLLATREAPTTWGATPYKDQ
                     RFDYDATVIRKLRDAGAILVAKLAMIELAGGMGYNQADASLTGACRTPWNTDYWSGGS
                     SSGPGAAVAAGLVPFAIGSETSGSIITPASFCGVTGLRPTYGMVSRHGAMALCWTLDK
                     LGPMCRTAADARIVLNAIAGRDPLDASSLERPRSRNSLNAAAAVNDRHKILRLAVMKD
                     SYEKAQKEVRENFLASLAALKRADRRVHVEMDVKLPDFPYSAAVGAIVDAEGAAAFRD
                     LIESGRVRELASPEGRIGGYSASLVTAVDYLHAMRLRVPMRRAWRELFLKYDVLVAPS
                     RATVSYPVNKTFDQAYPGIAASSPIGASNLVGVPAISIPNGFGQANLPTGIQFIGAAW
                     SEPTLLELADKYQQTTDWHKRRPTLA"
     gene            complement(11503..11895)
                     /locus_tag="VGB76_07880"
     CDS             complement(11503..11895)
                     /locus_tag="VGB76_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098858.1"
                     /translation="MKNREHDANASVHAQTTRRQFAKSIAAGVLFAPLTATTTSAQTP
                     PGAAKEPPAPPNPQPTPATASAAPQNAPSPVAEAYAEIGRLRFGEKLSADELVRMKRD
                     IEGNVRTAERLRAFKLKNADEPDFTFTA"
     gene            11924..12094
                     /locus_tag="VGB76_07885"
     CDS             11924..12094
                     /locus_tag="VGB76_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098859.1"
                     /translation="MKSSRTEKLQHGSNGLQTFRAPEHATRDSRRAAQADGASCRDEY
                     GSCAGLRMIRKA"
     gene            12133..12705
                     /locus_tag="VGB76_07890"
     CDS             12133..12705
                     /locus_tag="VGB76_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0098860.1"
                     /translation="MKINVRENPTLSLADYEAIPISFRVERVLRMERPPENAPGFIFS
                     EEPLDAPYVKDYDWPDSRPTVWAKHWDISKWGIFSAEEGGRLVGGAVVAWDTPEILQL
                     QGRRDICALWDIRVLPSHRGRGVGTALFKAAANWARSRGAVALRIETQTINVAACRFY
                     AAQGCTLAVVDLHAYPELPHETQLIWHLKL"
     gene            complement(12749..13147)
                     /locus_tag="VGB76_07895"
     CDS             complement(12749..13147)
                     /locus_tag="VGB76_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098861.1"
                     /translation="MSKPEKNWVEWSVFAVGLVLVLFALSYLVYEGATMGSSPPSIEA
                     RLGAPEQRAHNFIVPVSVTNHGDETAEGITIEVSLENPASPEGAQPIERGELTIAFLP
                     RRATREGWVTFRQDPRPARLTARVLGYEKP"
     gene            complement(13144..14088)
                     /locus_tag="VGB76_07900"
     CDS             complement(13144..14088)
                     /locus_tag="VGB76_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02587 family membrane protein"
                     /protein_id="HEY0098862.1"
                     /translation="MSGRKSTKNRTCRSVAESFQEYGRGVAGGLMFSLPLLYTMEVWW
                     AGFNSHPWRLAAYVLATFVLLLGYNRYAGLRHDASMWEVAIDSVEELGIGFIMSASAL
                     FILGRITLEMPANEIMGQIVIEAMTVAIGVSVGTAQLGGGGDAEGGMDDAQERPTRAS
                     GQAKPKDRGRNGGARSATRRREGEPHFGGQMLLALCGATLFASNVAPTEEIVLIAIES
                     SWPRLVALGLVSLLLGSLILHYSEFRGSQRFVRGGGLSAVVVGSVITYAVALFASALI
                     LWFFGRFDGVSLYTALAQIVVLGVAATLGASAGRLLLQ"
     gene            complement(14220..14765)
                     /gene="nusB"
                     /locus_tag="VGB76_07905"
     CDS             complement(14220..14765)
                     /gene="nusB"
                     /locus_tag="VGB76_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY0098863.1"
                     /translation="MGSRRKARECALQMLFAADVMSAARKDELRADQLVRYYWAELGE
                     TELDDPAREFATRLALGALSRVPELDERIRSRAEHWRIERMAVVDRNVLRLAVYEFLH
                     EPTPRTVAINEALEIARRFSTYEATQFINGILDAIKRDLDQEHPQTDVAHEDESTDEA
                     EDNSPDEDNEDDDEERTVTAS"
BASE COUNT         2732 a         4719 c         4737 g         2737 t
ORIGIN      
        1 tcacttgaga catgccctgc catgctcccg actggatgaa ggccgcccaa taaaaggggt
       61 ggctccgatt cccttcgccc cccgctaatc tgctcgccac taaggccgcg ttgctgcccg
      121 cctgaaacct tttgcgtgcc ggagagcatc gccagttgca cctccctgag cgcgtcgcat
      181 cgtccgagac ttaccacagg gtcatcacct gactctcgct tccggcgagc acgagcgcac
      241 gcctgaggcc atacacgtcg tcgccgttct gcacctcgcc cacgcccgtc tcgcacgccg
      301 acaacacgac gaggcgcgtg ccccagagat cgagactcgc ggcctcttag gctgtgcgag
      361 cggcgacggg ttacgagtcg gcttcgcccg cgtatttatc cgtgctcgcg ttggagatga
      421 cgggcgcatc ggtcgtcagc gcgttgttga cgagttcgcg ttgctccagt tcgtggatgg
      481 tgtaagagcc tgtggcgggg ctggccgagg ccgtgcgccc gacgtagtaa ggccgctcgc
      541 aattcttcat catgcccatc aggcgcggct cgacgaagtg ccaaccgccg taattcttcg
      601 gctcttcctg cgcccacacg agttgcgcgg cgttcgggta gcgcgcgaag atttcgcgca
      661 agaggcgttc ggggaacggg tagaactgtt cgagccggac gatggcgacg cgcctgtcgt
      721 ccgacttcaa acgcgccgcg ttcagatcgt aatagacctt cccgctgcac agcacgatgc
      781 gcaacacctc cgcgcggttc tctatctccg cgtcgtctat gacaggcagg aatccgcccg
      841 tcgtcaactc cgcgacggaa gaggtggcgg cgggcaggcg cagcaggctc ttcggcgtca
      901 tcacgacgag cgggcgcgcg atctcctgct tcacctgccg ccgcagaaga tggaaatatt
      961 gcgcgggcgt cgtcgggtaa cagacctgca tgttgttctc ggcgcagagt tggaggtagc
     1021 gttcgagccg cgcgttggag tgctccggcc cctgcccctc gtagccgtgc ggcaacagga
     1081 gcgcgaggcg tgaaggctgc tgccatttgt caacgccggg cgcgatgaat tgatctatga
     1141 tcacctgcgc gccgtttgag aagtcgccga actgcgcctc ccacatcacg agcgcgtcca
     1201 gagcctcgac cgaataaccg tactcgaagc ccatcacgcc ctgctccgaa agcgagctat
     1261 cgaagacttc aaagcgcggt cgcccgttct cgcgtgcggc gagttccgag agcggcgtcc
     1321 acgctctgcc cgtctgcgtg tcgtagagaa tggcgtggcg ttgactaaac gtgccgcgcc
     1381 cggaatcctg accgctcagg cggacgtttg tgccttcgag cgcgagcgag ccgaaggcca
     1441 cggcttcgcc gaacgcccag tccagaggcg tctgtcccgc ccccatcttc tcgcgccgcg
     1501 cgagttggct gaccatcttc gggttgacgt gaaaaccttc cggcacaatc gcaatgcgct
     1561 gcccgatctc gcgcaccaca tcggcggaga cgcccgtttc gaccacttcc gatccgtcct
     1621 cttcgactac cggagccgga acctccgaca tcttcggctt acccgcgacg atctctttcg
     1681 cacgcgcctg cgccccttcg taatgctcca cgcgcgcctt gatcatcgct tcgacatcgg
     1741 cctccgtcag cacgccttcg cggatgagcc gcccggcgta gatcgcgcgc acgcccggat
     1801 gcgctttcac gcgcgcgtac atcaaaggct gcgtgtaact cggctcgtcg ccttcgttgt
     1861 gtccgaggcg gcggaagccg atcacgtcca gcacgacatc cttgttgaac tcctgccggt
     1921 aatcgagcgc gatgcgcagc acgcggaaag cggtttcggg gtcgtcgccg ttgatgtgga
     1981 agatcgggag ttgcgtcatg cgcgccacgt ccgttgagta gatcgtggag cgtccctgtt
     2041 cgggcgaagt ggtgaaaccg atctggttgt tgatgatgat gtgaatcgtg ccgcccgtgc
     2101 gatagccgcg cagttcggcg aggttcagag tctccatcac gatgccctgc ccggcgaagg
     2161 ccgcgtcgcc gtgaagcagc acgggcagcg cacgatcaat cacctcttcg cgctccgcgt
     2221 tcatcgcgtc ctgtctggcg cgcaccatgc cttcgaccac cgcatcgaca aactcaagat
     2281 ggctcggatt cggcgacacc gtgatggcga cttcgcgccc gctcgccgtc tcgcgcctgc
     2341 cgcgcgcgcc ctgatgatat ttcacgtcgc cttcatcagc cggaaagttc ggatgcaccg
     2401 aaccctcgaa ggccgtgaag atgcgttccg agaaattgcc gaccacgttt gccagcacgt
     2461 tcagacgccc gcgatgcgcc atgccgagcg tgatctcttc gacgccgtgc tccgccgcct
     2521 tctcgatcaa ctggtgcagg agcggaatga tcgtctcgca gccttcgagc gagaaacgct
     2581 tcgcgcccag atacttcgtg tgcaggaaac gctcgaactg ttcggcggcg atcagacggt
     2641 cgagcagttg tttccgcgtc tcgaccggga gcggttcggg atagacgaac tcctgccgga
     2701 tgcgttcacg aatccaggtc ttctgttcct tgctctggat gtggcgatat tccgtgccga
     2761 ccttgccgca gtaggcgcgg cgcaggatgt ctaaaatctc cctcagcgta gagctttcgt
     2821 ggccgccgag tccgcccgtg atgaaggtgc ggtctaaatc ccagatggtc agcccgtagg
     2881 tttcgagatc gagttcggcg tgatgatgta cgggcatcgc atggagcgga tcgatgtctg
     2941 cgatgagatg gccgcgcacg cgataagcgt ttatgagttc gagcacgcgc gcctgtttga
     3001 cgatctgctc ctgcgtgcgg tcgccgccga ggagcgcggg attgcgatcc accgcccact
     3061 gcatcgggcg ataggcgatg ccgagatcga gaaagatgtt ctcgtaaaaa tcgtgcttgc
     3121 cgaccagaag ttcgtggacg tgcgcgagga acgcgcccga ctccgcgcct tgaatgatgc
     3181 ggtgatcgta ggtggaagag atcgtgagcg tcttgctgat gccgagttgc gagagggctt
     3241 ccggagacat cgcgtgatac tcggcagggt attcaatcgc gcccgtcgca atgatgaccg
     3301 actgaccggc catgaggcgc ggcgtcgaag cgacggtgcc gatggtgcca gggttcgtca
     3361 gcgagatcgt cgtctcctga aaatcgggca ggccgagttt gccgtcgcgc gcgcgcttca
     3421 ccacgtcgtc gtaagcgcgt atgaactgcg agaaggagag cgagttggcc gctttgatgt
     3481 tcggaaccag cagcgtgcgc gtgccgtcct tcttctgaat atcaatcgcc acgccgagat
     3541 tgactccggc gcgacgcacg cgcgcgggct tgccttcggc gagcgtgaag ccgtcattga
     3601 gttgcgggta cttgtcgagc gcgcgcagca gcgcgtaggc gatcacgtgc gtgtaagaag
     3661 ccttgccgcc gctctgctcc ttgagatgct tgttgatgat cgcgcgattt tcttcgagca
     3721 gtttgacggg cacctgccgg ttcgaggtcg cggtcgggac gctcaaactc gtctccatgt
     3781 tctcgacgat cttcaaggcc gcgccgcgta tcggggaggt ttccgccgta gacgcgccgg
     3841 gctgcgccgg ggcagcggcg gcagcggcgg cggtttgcgc cggggcagac tgtggctgcg
     3901 aggtcgcagg ctcaggcgtg gcggcgggag gttgcgcggg aacggcgacg ggcgcagccg
     3961 tttccgccgt gcgcgcggaa gtggcggcgg cagccgtcgc cgtgttgccg tccgcagggc
     4021 gcggcgcggc ggctgcgttc gtcgcgccgt tgccggaggt tgacgcggga gcggctgtct
     4081 cgccgagcat ctccatgaaa taagtgcgcc aagattcatc cacgagcgcg gggtcgctgc
     4141 ggaaacgctg caagagcgtc tcgacgtaag tggcgtttgc gccaaagttt tcggcgatga
     4201 attgcgagta gtcggtctgg ttttgttgtg cgctcaaagt ctgatcaccc cttttaagag
     4261 acgctcgggc ggcgggcggc tgtcgccgga gttccgccgc gttttactga cgattgttcc
     4321 gttggtgctt gaataatagc ctatcacttt ccgccttgca ctgcatccag cgcaaacttt
     4381 tgcgcaaccg cttcgtataa ttccacatca gatgaccgct atggaaaatg cgccgcgagt
     4441 cgaactcatg ccgtcgtcga aatttttcac gcacgccacg tttctctgtg tcctgctctt
     4501 cgcgtgcggc gcgtgcgcca tcccccgcgc agtctcacag cgcaagggag agactcctgc
     4561 gtcacgcgcg gcgacgccca cgccgcaagc cgccgttaag cccgtctatg aatcgcccgt
     4621 gctcgaacgt ctcgtcgcgg cggcggtcga gcgcacgacg catgaagtcg agtacgaccc
     4681 gacgtattac aagttggaat acccgaacgg cgacgttccg gcggacaagg gcgtctgcac
     4741 cgacgaggtg attcgttcgt atcgcgcgct cggcatagat ttgcagaaag aagtgcacga
     4801 ggacatgtct gccaacttct cgcgctaccc gacgaagttt ggcctgacga cgaccgacac
     4861 gaacatagac catcgccgcg ttccgaatct gcgcgtcttc ttcgagcgca aaggccgctc
     4921 gctccccatc acggacgaac cggcggacta caagcccggc gacatcgtga cctgggactt
     4981 gaacgcttcg cagacgcaca tcggcatcgt cgttgacgtg ccttcggcga cgcccggcag
     5041 gtacatgatc atgcacaaca tcggacaggg tccgaagatc gaagacatcc tcttcgcatg
     5101 gaagatcaca ggccactacc gttacacagg gacaaccgcg ccgccaccgg ccaagcccgc
     5161 accgcgtcct tcggcgtctc gacgcagcac acgctgaaaa aacacctcaa ggccaaagtg
     5221 aactctctcc tgtcctgaga tcgttgctcg tcccggttgc tctccgcagc agaaaaagat
     5281 tttcacctcc tgcaagtttc tcgtcccgcg cacattcatt tgagtgcaag cttgagaacg
     5341 ccgaaccaca gcgcacaggc gcaggaggat ttatgagcta ctcccgaacc atcacgcaca
     5401 cctcgcccga ctccaaacaa cgccgcccgc gccccgactc gccgcgcccg aagaacgcac
     5461 gcctgagttg gatcgcgctg tgcttcgtcg ccgcgctgct gctctcttcc tgcgaacgcg
     5521 cgagcgtgaa cgaaccggag cagaggccga gcaaagacgt gtcgaagacg gaaaataagg
     5581 cgcccctcgt cagtgcgccc gcgatgccta acccggagtt ggagatggcg cgagagacag
     5641 ccacaggctt gtatgctcaa cgccgcgacg gcgaggttga caacaagcag ttcaacaccg
     5701 aaagctacag ccacatcgac gagaaccctt ttttcgaggt cgcacgcgcg ccgctctcaa
     5761 ccttctcgat tgacgtggac acggcctcct acagcaacac gcgccgcttc ctgcgcgacg
     5821 gccaactccc gccgaaggat gcagtgcgca tcgaagagtt gatcaactat ttcccttacg
     5881 actacccgca gccacgaggc gacgcgcctt tctccatcaa cgcggaagtc tcttcctgcc
     5941 cgtggaacac aaagcgccgc ctcgtccaca tcggcttgca gggcaagcgc atctcgaagg
     6001 aagagttgcc gcccgccaat ctcgtctttc tcctcgacgt gtcgggttcg atgaacgcgt
     6061 cgaacaaact cccgctcgtt aaatcatcgc tgcgcatgct cgccgaacaa ctcgacgcgc
     6121 gcgaccggat cagcatcgtc gtctatgcgg gttcgagcgg gctggtgctg cccgccacgc
     6181 ccggcgaccg tcgcggcgag atcatcgccg cgctcgacgc gctcgacgcc ggaggctcga
     6241 ccaacggcgg cgaaggcatc caactcgctt ataaagtcgc ctctgataat ttcatacgcg
     6301 gcggtatcaa tcgcgtcatt ctcgccaccg acggcgactt caacgtcggc gtgacgagcg
     6361 aaggcgaact cgtgcggctc atcgaagaga aacgcaagag cggcgtcttt ctctccgtgc
     6421 tcggcttcgg cacgggcaac gtcaaagacg cgacgatgga gaaactgtcg gataaaggca
     6481 acggtaacta tgcctatata gacgacgtgc aggaagcgcg caaagtgctc ggcgagcaac
     6541 tcggcgcgac gctcgccacc atcgccaaag acgtgaagat tcaagtcgag ttcaacccgc
     6601 gacaggccac cgcctatcgt ctcatcggct acgagaatcg cctgctgcgc gatcaggatt
     6661 tcaacgacga cctgaaagac gcgggcgaga tcggcgcagg ccacaccgtc accgcgctct
     6721 acgaggtcgt cccctttggg cagaagttcg agaaccccgg cattgacgag ttgaagtatc
     6781 aaacgcccgt cgagtctccg gcacgcgcag acagcagaga gttgatgacg atcaagattc
     6841 gttacaaaga cccggaggag agcgcgagca aactcctgag cgtgtgggtg tcggatgcgg
     6901 gcggcacgct tgagagcgcg tcgaacaacc tgaaattctc ttcggcggtc gcaggcttcg
     6961 gcatgctcct gcgcgactcg aaatacaagg gcgacgtgcg ctacgcagac gttgcccggc
     7021 tcgcacgcgc tgccgcaggc gcggacacgc agggctaccg cacggagttt atccaactcg
     7081 tcgaacgcgc gcagacgctc tcaaggcaaa gcgcgcggaa ctgaacaaaa cgatgaacgc
     7141 ggaacgatgg acgatgaact gaaagcaagt tgtctttcat tccgcgttca tcgttccgcg
     7201 ttcatcgtct gtcttctccg cgtcccggcg gtgaaggttt ttacttctgc ctgcacactt
     7261 ccctacttga atctttcccg cccgtcggcg caaacgtcta taatcgcgtt cgatgaacga
     7321 accattgctg cgcgaatttc tggccgaggc cgacgagcgg atcgaactcc tgttcgcgga
     7381 gatcgaacgc ctgcgacgcg tccacgcaga agcggacggg cgcgcgcgcc gggagttgat
     7441 cgcgcgcatc tttcgccacg tccacacgct caaaggctcc gccgcaactc tcccccaact
     7501 cgccgccgtt agccgcctcg cgcatgaatt tgaaaacctc ctcgatgcca ttcgcgccgg
     7561 acgcgcgccg ctcacggacg agtccataga cgcctgcgaa gatgccacag acgcgctctc
     7621 cctcgccctc gcctcccacg cgcgcggcga cgaagatgca gcggaaccgc cgcccgccat
     7681 ctttcaacta ttgcgacaac acgcgcgcgg cgcgcctcaa ctttccatcg ctcaggaagc
     7741 aaacccgcgc acaggctccg tcgccgcttt gcccccggag atcacgcgtg cgctgtccgg
     7801 cgtcgagcag cgtcacctga gcgagtctgt gaaggaaggc gggcgggcgt tgctggtcgc
     7861 agcctctttt gatctcgcca cgctggacga acaattcggt cgtttcaacg ccgcgctcgg
     7921 cgcgtgcgga gagatcatct cgacgtttcc gggcgaccct gcgagcgcgc ccgaccagat
     7981 cagttttcgt gtcctctaca cgaccacctg cacgcgcgtc gaactcgaag aacgcctcgc
     8041 gccgttcgaa gcgaagctcg actggaggga agacgcgccc gaacccgacg gcgcgagcgc
     8101 gaacggcgca gaggcatcaa ccttcgacac ggacgccccg cccgaagcct ccacagactc
     8161 gacgcctgac ccatcaagcg tcgcgcccgc tgctacggcc acgcgtgcgc cgatttttgt
     8221 gcgcgtgcct ctgcacgaac tcgacgaact cgtgacagcc acgcacgagt tgttcacaga
     8281 gacgctggcc gcgctcgaac aaactcccgc cgtgccggag actgtcgctg ccgtcgctgc
     8341 cgctgaaacg actcacgcag ccggggcgac gggggcggag cgcgcacgcc gcctgcgcga
     8401 aaatttcagt gcgctggaag agcgcgtgat gagtctgcgc atgcagccgc tcatgcccgt
     8461 gctcgaacgc gcgcagcgtg cggcgcgtat cgccgcgcgt gccgccggga aagagatcga
     8521 gtttgaagtt gcgggcggcg acgtgcgcgt agaccgtgcg ctcgcggaac agatcggcga
     8581 accgctcctg cacctgctgc gcaacgcggt tgaccacggc atcgagactg ccgaagagcg
     8641 gcgggcgacg gggaagcctc tgcgcgggcg cgtgcgcctc gaagtggcga tggagggcag
     8701 ccgcgtgcgc gtgcgcgtgg ccgacgacgg gcgcggcatt gatacggagc gtgtggcacg
     8761 cgtggccgtc gcgtgcggct tgctcgaagc gggagcgcac gtcaccgatg aacaggcgtt
     8821 gcgcctgatc tttcgtccgg gcttctctac cgccgcagag gtttcggcca tgtcgggtcg
     8881 cggcgtcgga ctcgacgtgg tggagcacgc gttggagcgc gcgggcggcg agttgcgcgt
     8941 gcgctcggcg cggggcgcgg gtacgacctt cgagttgcgc gtgccgctca cgctcgcgct
     9001 cgtcgcggtc ttcgtcgtgc gcgcgcgcgg ctacgcttat gcgtttgatg cgagccacat
     9061 cgtcgccacg gcttatctcg aaccgtcgga gattgaaggc gttgagacgg ggcgcggcag
     9121 cgtgctctgg gagggcgagc ggctgccgtt cgtctccctg cggtcgctgc tcggagaggc
     9181 ttggaccccg gaggaagggg cgggcgaacg cttgcccgtc gtcgtcgtgc ggcgggagcg
     9241 agacggctcg tggcagagcg cgtccgcgcc ggagagtttc gtcattgcgg ttgatcgcat
     9301 cgaaggtgag cgcgaaccgc tggtgcgcag cctcggacgc catgcgacgc gctggcgcgg
     9361 cgtcagcggc gcgttcacag agcgcgacgg cacggtcgcc ctgctgctcg atctgccgag
     9421 gctgttggaa aaatagagcg cgcggcaaac gggcgagcga caatcaggaa gcacgcgtgc
     9481 gtcccgcgag cgaggcgatg gcggcgacgg taaagagcgc gacgccgacg aagacctgcg
     9541 gctgctgcat cagccagaga gccgcgccga agaggctcgc gcagatcaga ttcgccacga
     9601 cgcaaagcgc acgcaccaac ccatctccgg cagcgtgcct gagcgcgacc agatttagaa
     9661 agcccgcgaa catgagcgcg aaacccgcgc ccgcaaacca cagcgcgccg agcgtgaagg
     9721 cgtcgtagtc gtgaaaagtg aacaggaggt gcagcacgcc gagcgcgatg atgagatagg
     9781 tgcaggcggt gtggatcgtt ctgagcataa ttctcaaaag cgggaggcgg gatacagaag
     9841 tcagggtcag aatagaaacc agttgttctt attctgactc ttgaattctg tatcctgact
     9901 cctgccttct ctttccgtcc ttcatgcgag cgtgggtcgt cgcttgtgcc agtcggtcgt
     9961 ttgctggtac ttgtcggcca gttcgaggag cgtgggttcg ctccaggccg cgccgatgaa
    10021 ttggatgccg gtcgggaggt tggcttggcc gaaaccgttc gggatgctga tagcgggaac
    10081 gccgacgaga ttgctcgcgc cgatgggcga agacgcggcg atgcccggat aggcttgatc
    10141 gaaagttttg ttgacgggat aggagacggt ggcgcgcgag ggcgcgacga gcacgtcgta
    10201 cttgagaaac aactcgcgcc acgcgcgtcg catcggcacg cgcaggcgca tcgcgtggag
    10261 ataatcgacg gcggtgacga gcgacgcgga gtagccgccg atgcgtcctt cgggcgaagc
    10321 gagttcgcgc acgcgccccg actcgatcag gtcgcggaag gcggctgcgc cctcggcgtc
    10381 aacaatcgcc ccgaccgccg cgctgtaagg aaagtcgggc aacttcacat ccatctcgac
    10441 gtgcacgcgg cggtctgcgc gcttcaaggc cgcgagcgag gcgaggaaat tttcgcgcac
    10501 ctctttctgc gccttctcgt atgaatcctt catcacggca aggcgcagaa ttttatgccg
    10561 gtcgttcacg gcggcagcgg cgtttaaaga gttacgagag cgcggacgct ccaacgaaga
    10621 cgcatcgagc gggtcgcgcc cggcgatggc gttgagcact atccgggcgt cggcggcggt
    10681 gcgacacatg ggtccgagtt tatcgagcgt ccagcacaag gccatcgcgc cgtgacgcga
    10741 aaccatgccg taggtcggcc tcaagcccgt gacgccgcag aaggaggcgg gagtgatgat
    10801 tgagccggaa gtctccgagc cgatggcgaa ggggacgagt ccggcggcga ctgccgcgcc
    10861 gggtcccgaa gaggaaccgc cgctccaata atccgtgttc cagggcgtgc gacacgcgcc
    10921 cgtcaaactc gcgtccgcct ggttgtagcc catgccgccc gcgagttcga tcatcgcgag
    10981 tttggcgacg aggatcgcgc ccgcgtcgcg tagtttgcgt atgacggtcg cgtcgtagtc
    11041 gaagcgttga tctttgtaag gcgtcgcgcc ccacgtggtc ggggcttcgc gcgtggcgag
    11101 caggtctttc gcgccgtagg ggatgccgtg cagcagccct ctgtagcgac ccgcgcgaat
    11161 ctcgctctcc gcttgccggg cttcgttgag cgcgctccgg cgcatgatgg tcgccaccgc
    11221 gccgagccgc gcgccgaact gttcgaggcg cgcgaggtag ccttccgtca actcgacggg
    11281 cgagagtttg cgcgcacgca accgcgcggc cagttcgacg actgaagaga agaaggtgtc
    11341 ttcggtaagc ataagaatct ttgagcaggg aaaaggtgaa agggtacagg gtacagggaa
    11401 gaagccgcgc tgtcagtttg cgggatcgcg caaactgatc tttgagttcc gagctttgtc
    11461 tttccccttc tcccttaccc ctccaccttt cccctcacgt ctttaagcgg tgaaggtgaa
    11521 atccggttcg tctgcgttct tgagtttgaa ggcgcgcagg cgttccgccg tgcggacgtt
    11581 gccttcgatg tcgcgcttca tgcgcacgag ttcgtccgcc gacagttttt cgccgaagcg
    11641 caggcgaccg atctccgcgt aagcctctgc gacgggcgac ggcgcatttt gcggcgcggc
    11701 tgaagcggtg gccggggtcg gctgcgggtt cggcggcgcg ggaggttctt tggccgcgcc
    11761 gggcggcgtc tgcgcgctcg tcgtcgtcgc agtcaacggc gcaaacagca cgcccgccgc
    11821 gattgatttg gcgaactggc gacgcgtagt ttgcgcgtgg acgctcgcgt tcgcgtcgtg
    11881 ctctcgattt ttcatgtcaa acttttttcg caggggtgaa agtgtgaaat cttctcgcac
    11941 ggaaaagcta caacacggga gcaacggact gcaaactttt cgcgcgccgg agcacgccac
    12001 acgcgacagt cgccgcgcgg cacaggccga cggcgcgagt tgccgcgacg agtatggctc
    12061 gtgcgcgggt ttgcgtatga tacgaaaggc ttaacgcttt attgaccgcg cagcgacggg
    12121 agagacttat tcgtgaagat caacgtcagg gagaacccaa ccctctcgct cgccgactat
    12181 gaagcgatcc cgatctcctt ccgcgtggag cgcgtgttgc ggatggagcg tcctcccgaa
    12241 aatgcccccg gctttatctt ctccgaagag ccgctcgatg cgccttacgt caaagattac
    12301 gattggccgg acagccgccc taccgtctgg gcgaagcatt gggacatctc gaagtggggc
    12361 atcttctccg ccgaggaagg gggaaggctc gtcggcggcg cggtggtcgc gtgggacacg
    12421 ccggaaattt tgcagttgca gggcagacgc gacatctgcg cgctctggga catcagagtt
    12481 ttgccttcac accgtggccg tggcgtcggg acggcattgt tcaaagcggc tgcgaactgg
    12541 gcgcggtcgc gcggcgcggt cgccttgagg atcgagacgc agacgatcaa cgtcgcggcc
    12601 tgtcgcttct acgctgcgca aggctgcacg ctcgctgtcg tagacctgca cgcttatccc
    12661 gaactccctc acgagacaca gttgatctgg cacttaaaac tatgatgccc gcgctggccg
    12721 cgcctttcgt cacgggcgcg tgtgacgatc agggtttttc gtagccgagg acgcgggcgg
    12781 tgagacgtgc cgggcgcggg tcttgccgga aggtgaccca gccttcgcgc gtggcgcgac
    12841 gcgggaggaa ggcgatggtg agttcgccgc gctcgatagg ctgcgcgcct tccgggctgg
    12901 cggggttctc cagagagact tcgatggtga tgccttccgc ggtttcgtcg ccgtgattcg
    12961 tcaccgagac ggggacgatg aagttgtgcg cgcgctgttc aggcgcgccg agccgcgctt
    13021 cgatgctcgg cggactgctg cccatcgtcg cgccttcgta gacgagatac gagagcgcga
    13081 acagcaccag cacgaggccg acggcgaaga cgctccactc aacccagttt ttctccggtt
    13141 tgctcattgc agtaaaagtc ttccggcgga cgcgccgagc gtcgcggcta cgccgagcac
    13201 gacgatttgc gccagcgcgg tgtagagcga aacgccgtcg aagcgtccga agaaccacag
    13261 gatcagcgcg gacgcaaaga gcgcgacggc gtaagtgatg acgctgccga cgacgaccgc
    13321 gctcaaaccg cccccgcgca cgaacctttg cgaaccccgg aattcgctgt agtgaaggat
    13381 gagcgagccg agcagaagcg agactaaccc cagcgcgacg agacgcggcc acgaactctc
    13441 gatggcgatc aggacgatct cttcggtcgg tgcgacgttc gaggcaaaga gcgtcgcgcc
    13501 gcagagcgcg agcaacatct ggccgccgaa gtgcggttcg ccttccctgc gccgggtcgc
    13561 gctccgcgcg ccgccgttgc gccctcgatc ttttggcttc gcctgcccgc tggcgcgcgt
    13621 cggtctttcc tgcgcgtcgt ccatgcctcc ttccgcgtcg cccccgcctc cgagttgcgc
    13681 cgtgccgacc gagacgccga tggcgaccgt catcgcttca atcacgatct gtcccatgat
    13741 ctcgttggcg ggcatctcaa gcgtgatgcg ccccaagata aacagagccg acgccgacat
    13801 gatgaaaccg atccctaact cttcgaccga atcaatcgcc acttcccaca tgctcgcatc
    13861 gtggcgcaag cccgcataac ggttgtagcc gagcagcagc acgaacgtcg ccagcacgta
    13921 ggccgccagc cgccacgggt gcgaattgaa gcccgcccac cagacctcca tcgtgtagag
    13981 cagcggcagg ctgaacatca acccgcccgc cacgccgcgc ccgtactcct gaaaagactc
    14041 cgcgacgctc cggcacgttc tgttttttgt ggacttgcga ccagacatga gaaagcagtg
    14101 acgtgtgaca agcgacgtgt gacaagtggc gaacggggga gcgttgtcgg agcaaaggcg
    14161 aagcggcgcg gcgaggtggc gacgcgagag cgaggggcga taagttttcc ggcgagggtt
    14221 taggaggccg tcacggtgcg ctcttcgtcg tcgtcctcgt tgtcctcgtc gggcgagttg
    14281 tcttccgctt cgtccgtgga ctcgtcttcg tgcgccacgt cggtttgcgg atgctcttga
    14341 tcgaggtcgc gcttgatggc atcgaggatg ccgttgataa actgcgtggc ttcgtaggtg
    14401 gagaagcggc gggcgatctc cagagcttcg ttgatggcga cggtgcgcgg cgtcggctcg
    14461 tgcaggaact cgtagacggc cagccgcagc acgttgcggt caacgaccgc catgcgttcg
    14521 atgcgccagt gttcggcgcg cgagcggatg cgctcgtcga gttcgggaac gcgcgagagt
    14581 gcgccgagcg cgagccgcgt ggcgaattca cgcgccgggt cgtccaactc cgtctcgccc
    14641 aactccgccc agtaataacg cacaagctga tcggcgcgca gttcgtcctt ccgcgccgca
    14701 ctcataacat cagccgcaaa gagcatttgc aaggcacact cgcgcgcctt acgtctcgaa
    14761 cccatgaatt acaccccaga gtgacaagtg acgagtgaca agtgacgagt tgaagcaagt
    14821 tttatcttgt cactcgtcac gtgccactcg tcacttgtta aacgcttcct tgtaaagatt
    14881 gaccaactcg acagccgcca tggcggcctc aaaacctttg ttacc
//
LOCUS       DASWNS010000230         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_10174, whole genome shotgun sequence.
ACCESSION   DASWNS010000230 DASWNS010000000
VERSION     DASWNS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10174"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /gene="ttcA"
                     /locus_tag="VGB76_07910"
     CDS             complement(<1..750)
                     /gene="ttcA"
                     /locus_tag="VGB76_07910"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY0098864.1"
                     /translation="MFIESKIKTKLLRQMGEAIKDFGMISDGDRVMVCLSGGKDSYTL
                     LDLLLDVRERAPVHFDLLAVNLDQKQPNFPADVLPAYLASRGVPFRIVERDTYSIVKR
                     LVPEGKTYCSVCSRLRRGIIYSIAQEEGCTKIALGHHADDIITTFLLNFFFVGQLKAM
                     PPILRSNDGLNTVIRPLAYCRETEIAEYAALKGFPIIPCDLCGSQSNLKRARINRLVT
                     ELEREIPNVRSSMLTALGNAIPSHLLDPKLFD"
     gene            complement(1194..1886)
                     /locus_tag="VGB76_07915"
     CDS             complement(1194..1886)
                     /locus_tag="VGB76_07915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0098865.1"
                     /translation="MSNEMVEFTSNGGTTQGYLALPEGGGKGAGIVVIQEWWGLVPHI
                     KRVAERFAAEGFVALAPDLYHGRSTTSPDEAGKLMMALNIEQTEKDLRGAIDYLLAHE
                     RVAGETVGTVGFCMGGALSLYAATKNERVGACVVFYGGHPKIKFDLENMHAPLLGFYG
                     ERDDFATPEGARQLQATLQSLGKSAEIHIYEDADHAFFNDERPEVFNPAAAADTWQRT
                     LRFLREHLGETA"
     gene            2236..3021
                     /locus_tag="VGB76_07920"
     CDS             2236..3021
                     /locus_tag="VGB76_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY0098866.1"
                     /translation="MFRPGRNKDQPGGDHANEQSQQHQQQQQQQSQPTTSGYGASNAA
                     NADTTLPGSRSTSDQPSSTGARNMSETNPTTSRAVSESESLARDIKEGTLSGFVGNGT
                     TLTGEANFKGMLRVDGHLSGRVSSQDGTLIVSTGGQVDADVEVSVAQIYGTVVGNVTA
                     TKRIELGRVAKVTGDIQTPALVIENGAVFEGSCRMQHIKDAQAKEEGRPTSAATSAST
                     TGGASSTAAKPDGSKSSDGASTTASTGAGTPATGATRPSPATT"
BASE COUNT          649 a          939 c          942 g          589 t
ORIGIN      
        1 gtcgaagagt ttcgggtcga gcaggtgcga ggggatggcg ttgccgagcg cggtcagcat
       61 cgaactgcgc acgttcggaa tctcgcgttc caactcggtg acgagacgat tgatgcgcgc
      121 acgcttcaag ttcgactgcg agccgcagag gtcgcagggg atgatcggga atcctttgag
      181 cgcggcatac tccgcgatct ccgtctcgcg gcagtaggcg agcgggcgga taaccgtgtt
      241 cagcccgtcg ttcgaacgca ggatgggcgg catcgctttg agttggccga cgaagaagaa
      301 gttgaggaga aaggtcgtga tgatgtcgtc ggcatggtgg ccgagcgcga tcttggtgca
      361 gccttcctcc tgcgcgatgg aatagatgat gccgcgccgt agacgcgagc agaccgagca
      421 gtaagttttg ccttcgggca cgagccgctt gacgatggaa taggtgtcgc gttcgacgat
      481 gcgaaacggc acgccgcgcg acgcgagata agcgggcaga acgtctgccg gaaagttcgg
      541 ctgcttttga tcgagattga cggcgagcag atcgaaatgg acgggcgcac gctcgcgcac
      601 gtcgagcagc aggtcgagca gcgtgtagct gtctttgccg ccggagaggc agaccatcac
      661 gcggtcgccg tccgagatca tcccgaagtc cttgatcgct tcgcccatct gccggagcag
      721 tttcgtcttg attttgcttt cgataaacat cttcgttcgc gttctaactt aacgtgcggg
      781 cacgccgcgc aaagggtggc gcatcgcgtg cgatgtgcgc gcggctatcc ctgcaagttt
      841 tattcgatca ggcgcgcgga tgcaaccggg gcggagacgg gcggcgcaac ggcatcgcaa
      901 taaactttca acgcggctgc aaccccgccc cgccagcctt cgcctatagt ctctcccaac
      961 gaacattcct tcaatggaat aaaaaatcga aagcgtcggc ttgggggagt ctgacgcttt
     1021 cgttctcctt cgaatggtta gagctaggga agggcgcgca gaacttcgaa cggctgcgag
     1081 tctcagacgg taacaacaga gtcaacgcaa aagaaaaggg caaaccctaa cgccgggttt
     1141 gcccttttac tttcgccttc ttactttcgc cttcttactt ttggtagcgc gcctcaagcc
     1201 gtctcgccca gatgttcgcg taagaagcgc agggtgcgtt gccacgtgtc ggctgccgcc
     1261 gcagggttga agacttccgg gcgttcgtcg ttgaagaagg cgtggtcggc gtcctcgtag
     1321 atgtgtatct ccgccgactt gccgagcgat tgcagggtcg cctgaagttg gcgcgcgcct
     1381 tcgggggtgg cgaagtcgtc gcgctcgccg tagaagccga gcaggggcgc gtgcatgttt
     1441 tcgaggtcga atttgatctt cgggtgaccg ccgtagaaga cgacgcacgc cccgacccgt
     1501 tcgttcttcg tggcggcgta gagcgaaagc gcgccgccca tgcagaaacc gaccgtgccc
     1561 acagtctctc cggccacgcg ttcgtgcgcg agcaggtagt ctatcgcgcc gcgcaaatct
     1621 ttctccgtct gttcgatgtt gagcgccatc atcagcttgc ccgcttcgtc gggcgaggtg
     1681 gtggagcgcc cgtgatagag atcgggcgcg agcgcgacga agccttcggc ggcaaagcgt
     1741 tcggccacgc gcttgatgtg cggcacaagc ccccaccatt cctgtatgac gacgatcccg
     1801 gctcccttgc cgcccccttc gggcaaggcc agatacccct gagtcgtgcc gccgttgctt
     1861 gtaaattcca ccatctcgtt cgacatgaat ctactcctgt ctgaaaactc gacgttgatc
     1921 tttggcgtgt gttgagtgcc gttcaagtct aatccaaaag gcgcgaatcg ggaatcgtca
     1981 atcgtcagcg gataaaaccc ggactcaact ctcgacagcg ggcgagtcac cctccgaggc
     2041 tttatctcgt cacttgtcgc ttgtcacttg tcacgcgccc cgttatcatc acgggcaagt
     2101 cgtgtcgcag aagtttttga ggccgctacg gtcgagggtc cgagaagcat ccggcgcgaa
     2161 agagcttact acctgttttc catttccatc agccatcaga gcgcattaac cctaaagata
     2221 gcggagggag acaagatgtt tagaccggga agaaataaag accaaccggg cggcgatcac
     2281 gccaacgagc agtcgcagca acaccagcag cagcagcagc agcaatccca accgacgact
     2341 tccggctacg gcgcgagcaa cgccgctaac gcagacacga cgctgccagg gagtcgttca
     2401 acctcggatc agccctcttc aaccggagca cgtaacatga gcgaaaccaa cccgaccacg
     2461 tcgcgcgccg tcagcgaaag cgaatcgttg gcgcgcgaca ttaaagaagg cacactcagc
     2521 ggattcgtgg gcaacggcac gaccctcacg ggcgaagcca acttcaaagg catgcttcgt
     2581 gttgacggcc atctctcagg ccgcgtcagt tcgcaggacg gcacgctcat cgtctcgacg
     2641 ggcgggcagg tggatgccga cgtggaagtc tccgtcgcgc agatttacgg cacggtcgtg
     2701 ggcaatgtca ccgccaccaa gcgcatcgaa ctaggccgcg tggcgaaagt gacgggcgac
     2761 attcagacgc ccgcgctcgt catcgaaaac ggcgcggtct ttgaaggcag ttgccgcatg
     2821 caacacatca aagacgcaca ggccaaggaa gaagggcgtc ccacctcggc tgccacttcc
     2881 gcttcgacca cgggcggagc gagttcgacg gcggccaagc ccgacggctc caagtcgtcc
     2941 gacggcgcat caaccaccgc ctccaccggc gcgggcactc cggccaccgg cgcaacgcgc
     3001 ccgtcgcccg cgaccactta aaaaaagccg acccgaacga cagacgcgag aaaaggcaaa
     3061 agtgaagctc gaaagccgcc acgctttcac ttcacttttg ccttctcctt tttacctcg
//
LOCUS       DASWNS010000231        23009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_424641, whole genome shotgun sequence.
ACCESSION   DASWNS010000231 DASWNS010000000
VERSION     DASWNS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 23009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23009
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424641"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VGB76_07925"
     CDS             complement(<1..354)
                     /locus_tag="VGB76_07925"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEY0098867.1"
                     /translation="MEPLSSALAVLTAMITPAVLISACGALILSTSTRLGRVVDRVRA
                     LSDRFEELAVESCELALIEERRDVIFTLLDKLTSRARILQRSMTVFYIALSLFVATSV
                     AIGLVAYAGSRFSAVP"
     gene            599..1780
                     /locus_tag="VGB76_07930"
     CDS             599..1780
                     /locus_tag="VGB76_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098868.1"
                     /translation="MAEQANGGNGEDRDELTSGIEIGGGDDTATGGGGGGNSTDAESD
                     RVPVADNGRLDAAERRDAADEGGEGGVDDSSSTEGHGGTAIGRATGVTRGGRGGASKS
                     GSAGGGASGGGGGGASGGAGGGGGTSGKAKQGGAKGAGGSSASRAGGGNRVDAAEKGG
                     TAGRKNKGGGTQGGQSGSGSAQKGASKKGGAGGATGGGGGAGKGQHGGGGAKKGGTGA
                     ARTGGAKTGTGGGTKGGGGVGALKRGAGAARTGGATTETRGGAPGGGVAAGAGKSGAA
                     RATGTGGGKGGGLEQTGAAGKSGPKRETGGKKNALPSRAGGKGSPKGGAKSGGTGGVA
                     RGAGKNRGASGAAGGRGAPGGGAGGAGGDAKKGNKGGKGGGSGQNAGAPPKKGKGKSR
                     S"
     gene            2032..2946
                     /locus_tag="VGB76_07935"
     CDS             2032..2946
                     /locus_tag="VGB76_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0098869.1"
                     /translation="MRFWLSKNSEVPIREQLTTQIMLGVISNDLKPGQRLPSTRELAR
                     RFQIHANTVSAAYGELERRGWLESRKGSGVYVREFSKDRTLDPGFELDQLISAFLQQA
                     RDGGYSLGDVQTRIKRWLEFQPPDHLLVIEPDAELRAILVAEIEAATAFRVLGMSLEE
                     CAAHGLPTGAAAVALYGQAERAREVLPADTNRLIVLHSRSVPESLTGAVRPEADALIA
                     VVSRWPEFLRWARTVLVAAGIDPTALNFRDARQPGWQRGLTSSAFVITDALTARRLPP
                     NCTPRVFHMIADSSLDELRRFVRDFLTR"
     gene            3263..4501
                     /locus_tag="VGB76_07940"
     CDS             3263..4501
                     /locus_tag="VGB76_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098870.1"
                     /translation="MKRSSTPTFAFLIIVVFSFQTASAQFPRIPKIPKPDKPKSQPAA
                     PAAQPAQPAQPESRNEATPAPSSTSSGGQDQPTIAKDSVQLRAFTYNSYKGKYNTWSW
                     VPLISYRLNGPIPSGGQLYVEYTVPGAQPVRFDCRTEETPADRWWKTECGGREGVPEE
                     KATTYTGAFSFAIKMRNELAGADTTVFNGKAKIGKVHSNEIKTGEFANHFVYYVDHDW
                     NLPVGYVYYMPDDLKGWDRPILNVAFWVRGEAVRLDPHLFYQGKEVGRIMYEGEQVGK
                     ASCESDVENNTTHFVDEKDAPQKAKWARVRCTFNNVRGWDRSGERWGFGPPHLLDKNP
                     GEYELKVLWNNKLARSVKFNVPAGGKLDTSLAASNKLGTDRLIVPVTIIGDQDGAWDK
                     AAWKTDAFYGNPLTGFAAVP"
     gene            4739..6136
                     /locus_tag="VGB76_07945"
     CDS             4739..6136
                     /locus_tag="VGB76_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY0098871.1"
                     /translation="MLVGILTLALRVFFRRIEVKGLKHVAREGATIFVLNHPNALVDP
                     AFLLCYAPRRVSFLAKAPIFKMPIIGWLARALEAIPVYRRQDEGADVSRNQETFTRAR
                     NLLKRGGTLAICPEGVSHSDTKLRPLKTGAARIALGVVAEDAKLELKIIPAGLYYTAK
                     TAFRSAALLRFGEPIRVERAAPDANGEPPAEAVRALSARIERALHEVTLNAEHEQALA
                     IIKRAERIFSAAAGADEDGDLRTDAPSLEREVELQRRFLAGYTFHFERSPERLAALEA
                     RIHRYEEQLRQAGVDLEDLSAPAASPRQIARYFITRVVPVLYWFPLALFGILVHYPAY
                     KLAGALAVAVARRSDDVVSTIKLIAAMLLFPLTWGALAALFFYPLGWPGILLGLFLAP
                     VSAVVALLFFERLDGFVGGARAIRFYLTRRWFFKELLVERKRLREEMIALGEEAAAAG
                     SHAVVMPAAPTSNTI"
     gene            6235..8730
                     /locus_tag="VGB76_07950"
     CDS             6235..8730
                     /locus_tag="VGB76_07950"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEY0098872.1"
                     /translation="MPEDEHSQAADERPDHNGGAADSNGHVIARLEQLERAFGEQSKR
                     LRAIERRLGLVKYEPKSRPFAQGASTTSSETPAADAPESTQTRENFRANPYEEQASAP
                     HADVPPFNAPPPTGGTRPEADAAEVKTGATQASQGSLPARRDLEQLIGGGLFKWLGII
                     AVTLTVGFFLKVAFDSQLIGARGRVLIGGAAGCGILAFAERLRARGYKSYAHVLSGGG
                     ILILYLTVYAARVFYDLIGVVPAFLLMIAVTTAAVLLAARYDAYAIAVLGLIGGFMTP
                     VLLSTGVDNQLGLFSYIALLNSGVLALAYFKRWRSLNHLAFVATVLMFVGWSVVWYVE
                     TKLWPTLFFLTLFFLMFNALGVVYNVIKQRPAQWFDISLIISNATLFFAASYALLEPT
                     RHSILGSYALLWSAFYVLLFFFTYQRHRADKLLTLSYVGVAATFFTLAVAIQLDMHWV
                     TIAWAMEGLMLTWIGLRSDTDAPRYVALPVFACAIMHWFSTDMLEFAYRANETFVPLL
                     NRRAVSCAALVAALAGAFWLYRREGEKVERDQREIISALLVLAANVLLLTLLTVDAND
                     YFNARLASINPANYNADILAVENMATEQMALERMAIENARQFTLTVLWAFYALAALVV
                     GVRRNLLALRYAALAWLVVTGAKLLFADAEFYNAGWHLPLFNQTFAAFIAYVGALWYA
                     ARLYVHANKIDEYERRTTLNAITVVANLFAIIALSLEATGYFEKTVQFIGSDSPRLRD
                     LRLAQGLSLSLIWTLYGGAMLLYGHARKNRLLRLMALVLLTATAFKVFFLDLAVLEKF
                     YRIVSFLVLGAILLAVSFLYQQKQRGAAKVEGE"
     gene            8744..9472
                     /locus_tag="VGB76_07955"
     CDS             8744..9472
                     /locus_tag="VGB76_07955"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0098873.1"
                     /translation="MSEFSEVAATVEREGFAIVPGVIGEGEIARANAMLERAEHSDGV
                     RRRGGVYAIRNLLEAVPGLYELIEATPVRALAAQVLGAQCFVVRSILFDKTPEANWKV
                     AWHQDLSIAVRERRNAEGFGAWSEKAGVTHVQPPAEVLEGMLAVRLHLDASDEANAPL
                     RVLPGSHLRGRLSAEAIARMQEESEIVSCLVPRGGALLMRPLLLHASSAARVPFHRRV
                     VHLEFAAGELPAGLAWWSGAKSLS"
     gene            9486..10004
                     /locus_tag="VGB76_07960"
     CDS             9486..10004
                     /locus_tag="VGB76_07960"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0098874.1"
                     /translation="MSISDYQFISRWRVEGTCEEVSDVFRDVENLRRWWPATYLDVQV
                     LEEGDAQGVGKVVSLHTKGYLPYSLRWKFRVTESRYPYGSTLEAMGDLQGRGIWTFKQ
                     DGLLVDITYDWKVRARKPIVRALSLLLRPLLASNHQYAMRRGEESLKLELARRRARTP
                     EEAARIPPPPAK"
     gene            complement(10092..10418)
                     /locus_tag="VGB76_07965"
     CDS             complement(10092..10418)
                     /locus_tag="VGB76_07965"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEY0098875.1"
                     /translation="MLREETLTEPELKSFADFWPYYLAEHSHTGTRLLHAAGTITSTT
                     LAVALVATGRWRWLPAALATGYAAAWASHFLIEHNRPATFKHPLWSFAADYKMVALML
                     TGKLKG"
     gene            complement(10465..11601)
                     /locus_tag="VGB76_07970"
     CDS             complement(10465..11601)
                     /locus_tag="VGB76_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0098876.1"
                     /translation="MSIAGHATIEGTSRYRERMQAAITPNHFRLEQNLWLSSLGIGTY
                     LGKPDAATDARYTEAVTCAVELGANVIDTAANYRFQRSERSIGAALNALIESDDAATV
                     ARDEIVVCTKGGYIPFDGDPPNGQEGVRRYIETTFIKTGIASFSEIAGGSHCMTPKYL
                     AHQIDQSLRNMNLATIDVYYIHNPESQLGSVSRAEFETRLRAAFEQLERSVAEGKIKN
                     YGVATWNGFRVAPGAQGYHSLERMLELAREVGGEQHHFRFIQLPFNLAMPEALTVSNQ
                     TFQGAPVSVLEAARALEVTVVASASILQGKVARDLSPEIREPLGSLASDALTAIQFTR
                     STPGITTALVGMSRRAHVEENLQLTRIEPTRPEQYQQLFSTGEE"
     gene            11884..13449
                     /locus_tag="VGB76_07975"
     CDS             11884..13449
                     /locus_tag="VGB76_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0098877.1"
                     /translation="MNRAFNAETHEDAGGASLDITDEALRELSSRAVELVSDYFQSVS
                     ASPVLTHQGASVLDAQFDAPLPAAPDTLDELFADCRRIFEGSRHSGHPRFFGYVASPA
                     TPVGAFADLIASALNQNVPAWRSAPSATHVEQTVVRWLAALAGYSAEGASGLLTSGGS
                     MANLNALYVAHRSQARAKFDGGDDAAKRSGGDVSQKGLWQTARPATIYVSDQAHMSIA
                     KAADILGFGREQVRVVETDENFRMDVRRLRAAVERDARDNFEPCCVVASAGTVNTGAI
                     DPLAEIAELAREHDLWFHVDGAYGALAAADGTKRESLAGLARADSVALDPHKWLYTPV
                     DCGCLLFRDPARVRAAWAGSEADYIKIFEETERESFAFFDYGMELSRRFRALKIWMLL
                     RYYGAHRIAAAIAHDNALAAYFGARVEAAEDFELLAPVELSICCFRYVPPDMRRRLAT
                     ADETVRAGIEAELDELNERIMRAVQRGGRAYLSNASLRGRFALRACIINFRTTRRDIE
                     VTLEIVREAAREG"
     gene            13561..14379
                     /locus_tag="VGB76_07980"
     CDS             13561..14379
                     /locus_tag="VGB76_07980"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY0098878.1"
                     /translation="MAISLARAAGAAALAHYGGKLHIVNKSENEDPVTEADHAANDVI
                     VSGLQREFPADGLLSEESLDTVRRLNLARVWIVDPLDGTQGFIDGNGDFAVQIGLALE
                     GESVLGVVYQPVTGVLHYAARSAGAWVTRPEQQTPERLRVSDETEPSRMRLAASRNHH
                     SPRMAEVMRRLGLSEEIRRGSVGIKTGLIAERQCDLYVHLSSRTKHWDTCAPEAILRE
                     AGGRLTDLWGEPFLYNTEDVQNRNGLVAASGAAHPLIIERLAPLLREFGRTRVS"
     gene            14570..16597
                     /locus_tag="VGB76_07985"
     CDS             14570..16597
                     /locus_tag="VGB76_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0098879.1"
                     /translation="MNLRAKLLIVFIVGSLAPVLLVGGLFYRNSVGVVEGVLREEVEA
                     KTARIAAHVEAALRQREFDLDYLAQDSAMREYARSSAAPRPRAGAKNDARTGAGATVE
                     SPASLSPEAQDAIFDFLSRYRDYYSAITLISAPDEGRAPIRIESPTGADLNPRVKTSD
                     LLPGQMRLDERVWTSGERRSLRSSVKNAATGTPCIAYTLPVFSVENELTPRLALVAEL
                     RLDMLLQEAGSDLNAAAATSSAPAFSARVSEAGKYSRLLVVLDDAGEIVYHTNHAFKY
                     QPVSNAMPYFDATARAMQSGGTGTSFYTVPADGARWLASYKPVAGSNLSVAVAGNYTR
                     AVSSLNRNALLVLSLSLLIMAAAALLLFAVATRAALRIRRVTEAAAAIATTGDLNQRI
                     EVQANDETHALAEGFNRMSDRLREFIAREAEMRQFQSFVRISAMLTHDLKNSITGLSM
                     LVLNMERQFHRAEFREDAISSLKQATEKLGGLVARLSEPVKSLSGEYRRTPQPADLVP
                     VIRRVMAASAEPSAAFYDIQTELPPTLFALIDADRIERVVENLVVNALEAMGGRGGRL
                     TVTAGEESEGLVFFSVEDTGPGMTEDFMRTRLFRAFATTKKRGIGLGLYTCREIVESH
                     GGRLEVKSKLGSGTRFRVVLPSISLMASHARSFDKPPRNKEAKTTKTGSAR"
     gene            16738..18159
                     /locus_tag="VGB76_07990"
     CDS             16738..18159
                     /locus_tag="VGB76_07990"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0098880.1"
                     /translation="MSEHTPEQTPDKATVLVVDDDQVLRRQLFWALDSEHHVLEADSR
                     IAAIAQLQSGRVGVVLCDLHLPPDLDGISEGLAIIEAARAARPSIPVIVITGSNSRQT
                     ALEAIRCGAYGFFEKPFNEDEVTHIVRQAARVRHLEEENLHLRASLGSFRGFGRLLGS
                     SPALERIMKQARAVASTSATVLLTGENGTGKEMLARAIHEESARVDQPFIAVSCAALP
                     ESLIESELFGHEKGAFTGAVAAKKGRFELADGGTLFLDEIGELSQAVQVKLLRVLQER
                     NFERLGSTKTQTVDIRLIAASNRDLEQEVEEGRFRRDLFYRLNVVPLQLPALRERRED
                     LPILAAHFASRAAEKHGRPTPALEPALLEALQEYDWPGNVRELENLMERLIVLTTGTK
                     LGIEFLPERMLHLRRPVAPEAGRNGENLYDTTLEGAVLALKRQLVINALQAESGNKAA
                     AAKRLAISRSYLHRLVSELNIPT"
     gene            18509..18901
                     /locus_tag="VGB76_07995"
     CDS             18509..18901
                     /locus_tag="VGB76_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098881.1"
                     /translation="MKPKLLIVDDDSAIRQQLFWTLCDEYDVIMASDFPSAMRHATSS
                     EPVISLLDLHMPPVLDSPEVGLRLLDYIKEQCPSSKVYIVSSEESIETKKACFKQGAD
                     GFLSKPLDVEQLLTTVRHSTLAARLKAA"
     gene            18959..19165
                     /locus_tag="VGB76_08000"
     CDS             18959..19165
                     /locus_tag="VGB76_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098882.1"
                     /translation="MKAILKRTDADGARELTPAEIRGVLLKLARKKISERVARPAIDA
                     LNKIMCDALAASQPLRATQGKHEG"
     gene            19567..20997
                     /locus_tag="VGB76_08005"
     CDS             19567..20997
                     /locus_tag="VGB76_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEY0098883.1"
                     /translation="MKFLHLADIHLGCRRYNVEERTTDFFRAWRDVIEQHALPNAVDF
                     VLICGDFFNSRKVDPQAMNHAIIGLQRLKEAGIPVVAIEGNHDQHESDAVNRFSWMRS
                     LSQWGFLTLLEPDTRDGFNLTPWNEEERSGSYIDIAGARIFGTHWFGTSTNAAIPLLT
                     DALRRARGENLFNILMLHTDVEGQLNRPNIPALSISRMKELRTLVDYVALGHTHKRFE
                     IEDWAFNPGSLEACTIDEFKEERGLYLVEVDEAHRVTAHHSRDYTQRPFQRLNFDVSG
                     VADAEAVHEGVLEVLRREARTHDPERDETPAPIIEINLRGHLGFKNSLLDIPRMREEA
                     RTLTGALHIMISNRSVPVEYAVAAGLDSDATRQVRERRIIEDLITRDIRFRHRAHDMA
                     ALTLEAKRLALGDESPEKILELIEQQLYTDAEQPNGASSNAMSDAAAGDTAGTPIATD
                     DTELVASASALQAIERNAMKAVTSDK"
     gene            21070..21774
                     /locus_tag="VGB76_08010"
     CDS             21070..21774
                     /locus_tag="VGB76_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY0098884.1"
                     /translation="MPDSFERVRGVSELPLFPLPLVLFPGVPMPLHIFEERYRRMLAD
                     VRAGNNLFGLSYFDLNTATNTRPAPGHVGCAAEIVEAQLLPDGRSNILTVGLIRYRLV
                     EYVELGEPYLVGRVEFFEDEPEDEALLLERAHEVAELFMRIARAVRNLNDDRAGLPEL
                     PDAEPERLSFLVAAAMELDAEAKQGLLELRSGAQRLERIGNLLSQVVGNYEERARTHK
                     LARGNGHGGNHLNLDQ"
     gene            21771..22568
                     /gene="moaD"
                     /locus_tag="VGB76_08015"
     CDS             21771..22568
                     /gene="moaD"
                     /locus_tag="VGB76_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEY0098885.1"
                     /translation="MSSPGNEPTRQTNDSPQTIRVRLLYFGAAREEVGREEEQLEVTS
                     PATSASVLAEVLNAHPALRRFGRSLLVAVNEEYAGASARVEAGDEVAIFPPVSGGAGG
                     REGEGVGGGVEAGEREACAAEDFFELTTDPINVGAVARRVVPPACGATVTLDGYVRRF
                     TRGREATLYLIYEAYAPMALSEMRRLGRQIHERFEVAHVGIVHRTGRLEIGETSVVIS
                     VSAPHRRAAFEACEWAIRELKRTVPIWKKEFFAGGEVWIEGEGAPVN"
     gene            22577..>23009
                     /locus_tag="VGB76_08020"
     CDS             22577..>23009
                     /locus_tag="VGB76_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY0098886.1"
                     /translation="MKKLDGLLARNRAWAKKMTTADPDFFKRLSQQQTPEYLWIGCSD
                     SRVSANEIIGLLPGEIFVHRNVANVVVHTDLNCLSVIQYAVEVLKVKHIIVCGHYGCG
                     GVKAALDNGKHGLIDNWLRHVQDVQRDHQKELRAIAGDAARV"
BASE COUNT         4427 a         7333 c         7135 g         4114 t
ORIGIN      
        1 gggcactgcg ctgaagcgcg aaccggcgta ggcgacgagg ccgatggcga cgctcgtggc
       61 gacgaagaga ctgagggcga tgtagaagac ggtcatgctg cgttggagga tgcgcgcgcg
      121 gctcgtcaat ttgtcgagca gggtaaagat cacgtcgcgg cgttcttcga tgagcgcgag
      181 ttcgcagctt tcgacggcca gttcctcgaa gcggtcggag agcgcgcgga cgcgatccac
      241 gacgcgaccg agccgcgtgg aagtggacag gatgagcgcg ccgcacgccg agatcaaaac
      301 ggcgggcgtg atcatggccg tcaggacggc gagcgcggaa gagagcggtt ccatgtcggt
      361 tgttgcttga agcgaactcc gttatttata acgcccgccc cgtgtgtgag caaccggcgg
      421 cgggcgcgcg ggaaaatttt tgcgcgcggc ggggaaagag ttgaacgcgt gcgcgggaag
      481 ttgtgctatg ctccgccgca aatcgtaact cgaaaatttc agcaggccgg aaggaatctt
      541 atggcaagac ctcgcgcgcg gggtcgcacc tcttcacatc agaacaagga gcaaagtgat
      601 ggctgaacaa gcgaacggtg gaaacggtga agaccgggat gaactgacga gcggcatcga
      661 gatcggcggc ggcgacgaca cggcgacggg tggcggcggg ggcggcaact cgaccgacgc
      721 cgaaagcgac cgcgtgcccg tcgcggataa cggtcggctg gacgccgccg agaggcgcga
      781 cgccgcagac gaaggcggcg aaggcggcgt tgacgacagc agcagcaccg aaggacacgg
      841 cgggacggcc atcgggcgcg cgaccggagt gacgcgcggc ggcagaggcg gcgcgtcgaa
      901 gagcgggagc gcagggggcg gcgcgagtgg cggtggcggt ggtggcgcga gtggtggcgc
      961 gggcggtggc ggcggcacaa gcggcaaggc caagcagggc ggcgcgaaag gcgcgggcgg
     1021 gtcgagtgct tcgcgcgcgg gcggtggaaa ccgtgtcgat gcggcggaga agggcggcac
     1081 ggcgggtcgc aagaacaaag gcggcggcac gcagggcgga cagagcggca gcggttcggc
     1141 gcaaaagggc gcgtccaaga agggcggcgc gggcggcgca acgggtggag gtggcggtgc
     1201 gggcaaaggc cagcacggcg gaggcggcgc gaagaaaggc ggcaccggcg cggcgcgcac
     1261 gggcggcgcg aagaccggca cgggcggcgg cacgaagggc ggtggcggcg tcggcgcgct
     1321 caagcgcggc gcaggcgcgg cgcgtaccgg aggcgcaacg acggagacgc gcggcggcgc
     1381 accgggtggc ggcgtggcgg cgggcgcagg caagagcggc gcggcgcgtg cgacgggcac
     1441 gggcggcggc aaaggcggcg gactcgaaca gacgggcgca gccggaaaga gtggtccgaa
     1501 gcgcgaaacg ggcggcaaga agaacgctct gccatcgagg gcgggaggca aaggcagccc
     1561 gaaaggcggc gcgaagtcgg gcggcacggg cggcgtcgca cgcggcgcag ggaagaatcg
     1621 cggcgcgtcg ggcgcggctg gcgggcgcgg cgcgccgggc ggcggtgcag gcggcgcggg
     1681 cggcgacgcg aagaaaggga acaagggcgg caagggcggc ggctcaggcc agaacgccgg
     1741 agcgccaccg aaaaaaggaa aaggcaaaag tagaagctga tcaaagtctg atcacgaccg
     1801 tcgccgaaac gacgggcaga gtcgcggcgc gcgtcacgaa cgtcatcggc agtctgacgg
     1861 gcgcgcttag aaaagggcgc aaaggttagc gcgcgaggtt agcctgcaaa gttagcccgc
     1921 aaggtcagtt tgtgccgcga cgagaacaag cagggaggtc gcctttcgtg tcgcacggat
     1981 acgaaaggcg acctcttcgc gcaaaaggag cgaccgtccg tagcgttact catgcgtttc
     2041 tggctctcca aaaatagcga ggttccgatc cgcgaacagt tgacgacgca gatcatgctc
     2101 ggcgtcatca gcaacgatct caagcccggc caacgcctgc cgagcacgcg cgaacttgcg
     2161 cgccgttttc agattcacgc caacacggtc agcgcggcct acggtgaact cgaacgtcgc
     2221 ggctggctcg aatcgcgtaa gggaagcggc gtctacgtcc gcgagttctc gaaagaccgc
     2281 acgctcgacc ccggattcga actcgaccaa ctcatctccg ctttcctcca acaagcgcgc
     2341 gacggcggct actcgctcgg cgacgtgcag acgcgcatca aacgctggct cgaattccaa
     2401 ccgccagacc atctcctcgt catcgaaccg gacgcggaac tgcgcgccat tctcgtcgca
     2461 gagatcgaag ccgcaaccgc gtttcgcgtg ctcggcatga gtctggaaga atgcgcggcg
     2521 cacggcctgc ctacgggcgc ggcggcggtc gcgctctacg ggcaggcgga gcgtgcgcgc
     2581 gaggtgctgc ccgccgacac gaaccgcctc atcgtgctcc attcgagaag cgtgccggag
     2641 tctttgacgg gcgcggtgcg tcccgaagca gacgcgctca tcgcggtcgt ctcgcgctgg
     2701 cctgaatttt tgcgttgggc gcgcacggtg ctcgttgccg caggcatcga cccgaccgcc
     2761 ctgaacttcc gcgacgcccg gcagcccggc tggcaacgcg gcttaacgtc gagcgcgttc
     2821 gtcatcaccg acgccctcac cgcgcgccgc ctgcccccga actgcacccc gcgcgtcttc
     2881 cacatgatcg ccgactcgtc gctcgacgaa ctgcgccgct tcgtccgaga cttcctcacg
     2941 cgctgagcac cggcgcggtt ttcgtcgccg cgaatcgccg ccgtcgaaac ggaggccgga
     3001 cggttttgcc gggcgataga tattgacagg tgcaaacgac agtcggatac tgctccgcac
     3061 cgagccgggg tttctaatcc gcgcgccgaa tttcaaccct cacaggcgca cgcaaaacga
     3121 cacgcaggga acacagacgc gcccgaccgc ccgcgagcga tgccgcccac gggagagact
     3181 ttttcgtcgt tcccgtttga gtgttctgtc ggcaggtcgt ccagactttg gagtgatcac
     3241 agagacaaag gagagattaa agatgaaacg gagttcaacg cccactttcg cgtttttaat
     3301 catcgtcgtt ttcagctttc aaacagcgtc ggctcaattc cccaggattc cgaagattcc
     3361 gaaacccgac aaaccgaaat cgcaacccgc cgctcccgcc gcgcaacccg cacagcccgc
     3421 gcagccggag agtcgaaacg aggcgacacc cgcgccttcg tcaacctcgt ccggcggtca
     3481 agaccagccc accatcgcca aagactccgt gcaactgaga gccttcacct acaactccta
     3541 caagggcaaa tacaacacct ggagttgggt gccgctcatc tcctaccgcc tcaacggccc
     3601 catcccgagc ggcggccaac tgtacgtcga atacaccgtg ccgggggcgc agcccgtcag
     3661 gttcgattgc cgcaccgagg agacgcctgc cgaccgctgg tggaagactg agtgcggtgg
     3721 ccgcgagggc gtccccgaag agaaggccac gacctatacc ggcgccttca gcttcgccat
     3781 caagatgcgc aacgaactgg ccggtgccga cacgacggtc ttcaacggca aggcgaagat
     3841 cggcaaggtg cactccaacg agatcaagac cggcgagttc gccaaccact tcgtctacta
     3901 cgttgaccac gactggaacc tgcccgtcgg ctatgtctac tacatgcccg acgacctgaa
     3961 gggctgggac agaccgatcc tcaacgttgc cttctgggtg cgcggcgagg ccgtgcgcct
     4021 cgacccgcac ctcttctatc agggtaagga ggtggggcgg atcatgtacg agggcgaaca
     4081 ggtcggtaag gccagttgcg agtcggacgt tgagaacaac acgacgcatt tcgtggacga
     4141 gaaggacgcg ccgcagaagg cgaagtgggc gcgcgtccgc tgcaccttca acaacgtgcg
     4201 cggctgggac aggtcgggcg agcggtgggg cttcgggcct ccacacctgt tggacaagaa
     4261 ccccggtgag tacgagttga aagtgctctg gaacaacaag ctggcgcgct cggtcaagtt
     4321 caacgtcccg gcgggcggca aactcgacac cagcctcgcc gccagcaata agctcggcac
     4381 cgaccgcctc atcgtccccg tcacgatcat cggagatcag gacggcgcgt gggataaggc
     4441 ggcgtggaag accgacgcct tctacggcaa cccgctcacg ggcttcgcgg ctgtcccgta
     4501 agccggaagg cgggggcttg ctgtgtcaac tggaagcccc cgcccgtctt gcctgagaag
     4561 tttcccgtct cttccgcttc tcgcctcttc gatgtgacac ttcgcaaagc cgcacaaagc
     4621 tctagccgcc tctccgtcgc ttgttctata tttcgtgtgg ctcgcaggcg cgagaaacgc
     4681 gcatcgaatg ttttcatagg tcgtgcgtga aaggagttgt caaactgtgg tgaggcgaat
     4741 gttggtcgga attctcacgc tcgcgctgcg cgtcttcttc cggcgcatcg aggtgaaggg
     4801 cttgaagcac gtcgcgcgcg aaggcgcgac catcttcgtg ctcaatcatc cgaacgcgct
     4861 cgtcgatccg gccttcctcc tgtgctacgc gccgcgccgc gtctctttcc tcgccaaagc
     4921 tcccatcttc aaaatgccca tcatcggctg gctcgcacgc gcgctcgaag ccatccccgt
     4981 ctatcgtcgt caggacgaag gcgcggacgt gtcgcgcaat caggagactt tcacgcgcgc
     5041 acgcaacctg ctcaagcgcg gcggcacgct cgccatctgt cccgaaggcg tctcgcacag
     5101 cgacacgaaa ctgcgccccc tgaaaacggg tgcggcgcgc atcgcgctcg gcgtcgtcgc
     5161 cgaagatgcg aaactcgaac tcaagatcat ccccgcaggt ctgtactaca cggcaaagac
     5221 ggcctttcgc agcgcggcac tgctccgctt cggcgaaccc atccgcgtcg aacgcgccgc
     5281 tcccgatgcc aacggcgaac cgcccgccga agccgtccgc gcgctctccg cacgcatcga
     5341 acgcgccctg catgaagtga ccttaaacgc tgaacacgaa caggcgctcg ccatcatcaa
     5401 acgcgccgaa cgcatcttct cggcagccgc aggcgcggac gaagacggcg acttgcgtac
     5461 ggacgcgcca agtctcgaac gtgaagtaga actacaacgc cgtttcctcg cgggctacac
     5521 cttccacttc gagcgttcgc ccgaacggct cgccgcgctc gaagcgcgca tccatcgtta
     5581 cgaagaacaa ctccgacagg ccggggttga cctcgaagac ctgtccgcgc ccgccgcctc
     5641 gccccgtcaa attgcgcgct atttcatcac gcgcgtcgtg cccgtgctct actggtttcc
     5701 gctcgcgctc ttcggcatcc tcgtgcacta cccggcctac aaactcgcgg gagccttggc
     5761 cgtcgccgtc gcgcgccgca gcgacgatgt cgtctccacg atcaagttga tcgccgcgat
     5821 gctcctgttc cctttgacgt ggggcgcgct ggccgcactc ttcttctacc cgctcggctg
     5881 gccgggcatc ctgctcggac tctttctcgc ccccgtctcc gccgtcgtcg cgctgctctt
     5941 tttcgagcgg ctcgacggct tcgtcggcgg cgcgcgtgcc atccgttttt acctgacgcg
     6001 tcgctggttc ttcaaagaac tgctggtcga gcgtaagcgt ctgcgcgaag agatgatcgc
     6061 gctcggcgaa gaggccgccg ccgccggaag ccacgctgtg gtgatgccag ccgccccgac
     6121 aagtaataca atctgagcga acgacgaaac tgatccggcg tagagagacg ggcgtagtgt
     6181 cgccatgccc tccttctcga cgcacttatt cccgaactcc ggcgcggagc ttttatgcct
     6241 gaagacgaac attcgcaagc ggcggacgaa cgccctgatc ataacggcgg cgcagccgac
     6301 tcgaacgggc acgtcatcgc gcgcctcgaa caactcgaac gcgccttcgg cgaacagtcc
     6361 aagcgtctgc gcgcgattga gaggcggctc ggcctcgtca aatacgagcc gaagtcgcgc
     6421 cccttcgcac agggcgcttc gacgacttca agcgagacgc ccgcagccga cgcaccggag
     6481 agcacgcaaa cgcgcgaaaa cttccgcgcg aacccgtacg aagagcaggc gtctgcgccg
     6541 cacgcagacg ttcctccttt caacgcgccg ccgccgacgg gcgggacgcg accggaagcg
     6601 gacgccgcag aggttaagac gggcgcgacg caggcatcac agggcagcct gcccgcgcgc
     6661 cgcgatctgg aacaactcat cggcggaggt ctgttcaagt ggctcggcat catcgccgtc
     6721 acgctgacgg tcgggttttt cctcaaggtc gcgttcgata gtcaattgat cggagcgcgt
     6781 ggccgtgtcc tgatcggcgg cgcggcgggc tgcggcatcc tcgccttcgc cgaacgcctg
     6841 cgcgcacgcg gctacaaaag ctacgcacac gtgctctccg gcggtggcat cctgattctc
     6901 tatctcaccg tctatgccgc gcgcgtcttc tacgatctga tcggcgtcgt ccccgcgttc
     6961 ctgttgatga tcgccgtgac gaccgccgcc gtgctgctcg ccgcgcgtta cgacgcttac
     7021 gccatcgccg tgctcggcct catcggcggc ttcatgacgc ccgtgctgct ttcgaccggc
     7081 gtggacaatc aactcggact cttcagctac atcgcgctcc tgaattccgg cgtgctcgcc
     7141 ctcgcttact tcaaacgctg gcgcagtctg aaccatctcg ccttcgtcgc caccgtgctc
     7201 atgttcgtgg gttggtcggt cgtctggtat gtcgagacga aactctggcc gacgctcttc
     7261 ttcctgacgc ttttcttcct catgttcaac gcgctcggcg tcgtctacaa cgtcatcaag
     7321 cagcgacccg cgcagtggtt cgacatctcg ctcatcatct cgaacgcgac gctcttcttc
     7381 gccgcgagct acgcgctcct ggaaccgacc cgtcactcga tcctcggttc ctacgcgctc
     7441 ctgtggtcgg ccttctacgt cctgctcttc tttttcacct atcaacgaca tcgcgcagac
     7501 aagttgctca cgctctctta cgtcggcgtg gccgccacct tcttcacgct cgccgtcgcc
     7561 atccaactcg acatgcactg ggtgacgatt gcgtgggcga tggaaggctt gatgctcacc
     7621 tggatcggcc tgcgctccga cacggacgcg ccccgttatg tcgcgctccc cgtcttcgcc
     7681 tgcgccatca tgcactggtt ctccacggac atgttggagt ttgcctatcg cgcgaacgag
     7741 acgtttgtgc cgctcctcaa tcgtcgcgcc gtctcctgcg ccgcgctcgt cgccgcgctg
     7801 gccggggcgt tctggctcta ccgccgcgaa ggcgaaaaag ttgagcgcga ccagcgagag
     7861 atcatcagcg cgctcctcgt cctcgccgca aacgtgctgc ttctgactct gctcaccgtt
     7921 gacgccaacg actatttcaa cgcgcgcctc gcgtcaatca acccggccaa ctacaacgcc
     7981 gacattttag ccgttgaaaa catggcgact gaacagatgg cgttggaaag aatggcgatt
     8041 gaaaacgcgc gccagtttac tttgaccgtg ctgtgggctt tctacgcgct cgcggctctg
     8101 gtcgtagggg tgcggcggaa cctgctcgcg ctgcgctacg ccgcgctcgc gtggctcgtc
     8161 gtgacgggcg cgaaactcct cttcgccgat gcggaattct ataacgccgg gtggcacctg
     8221 ccgctcttca atcaaacctt cgccgccttc atcgcctatg tcggcgcgct ctggtatgcc
     8281 gcgcgcctct acgtgcatgc gaacaagata gacgagtacg agcggcgcac gacgctcaac
     8341 gccatcacgg tcgtcgcgaa cctcttcgcc atcatcgcgc tcagcctcga agccacgggc
     8401 tacttcgaga agacggtgca gttcatcggg tctgacagcc cgcgtctacg cgacctccgg
     8461 ctcgcgcagg gactttcgct ctcgctcatc tggacgctct acggcggcgc aatgctgctc
     8521 tacggccacg cgcgcaaaaa ccgcctcctg cgcctcatgg ctttagtgct tttgacggcg
     8581 acggccttca aggtcttctt cctcgatctg gccgtgctcg aaaagtttta tcgcatcgtc
     8641 tcgttcctcg tgctcggcgc gatcctgctc gccgtctcgt tcctctatca acagaaacag
     8701 cgcggcgcgg cgaaggtcga aggggaatag cggcagaggt tttatgagcg agttctccga
     8761 agttgccgcg acggtcgagc gtgaagggtt cgccatcgtg cccggcgtga ttggagaggg
     8821 tgagatcgcg cgcgccaacg caatgcttga gcgtgcggag cactcggacg gcgtgcggcg
     8881 tcgcggcggt gtctacgcta tccgcaactt gctggaagcc gtgcccggac tttatgagtt
     8941 gattgaagcg acgccggttc gcgcgctcgc ggcgcaggtg ttgggcgcac agtgtttcgt
     9001 cgtccgcagt atactgttcg acaagacgcc ggaggcgaac tggaaagtgg cctggcatca
     9061 agacctctcg attgcggtgc gcgagcggcg aaacgcggag ggcttcggcg cgtggtcgga
     9121 gaaagcaggc gtgacgcacg tgcaaccgcc tgccgaagtt ctggaaggca tgctcgccgt
     9181 caggcttcac ctcgacgcga gcgacgaagc caacgcgccc ttgagagtgc tcccaggttc
     9241 acacttacga ggccgcctga gtgcggaggc catcgcgcga atgcaggaag agtcggagat
     9301 cgtctcgtgc ctcgttccgc gcggcggggc gttgctgatg aggccgttgc tcctccacgc
     9361 ttcttccgca gcgcgcgtgc cgtttcatcg ccgcgtcgtc catctggaat tcgccgccgg
     9421 agagttgccc gccgggttgg cgtggtggag cggcgcaaag agcctgagct agagagaata
     9481 ttccgatgtc aatttccgat tatcaattca tctcgcgctg gcgcgtcgaa gggacgtgcg
     9541 aggaggtttc agacgtgttc agagacgtgg aaaatctgcg tcgctggtgg cctgcgacgt
     9601 atctggacgt gcaggtgttg gaagagggcg acgcgcaggg cgtggggaaa gtagtcagcc
     9661 tgcacacgaa ggggtatctg ccttactctc tgcgctggaa atttcgcgtg accgaatcgc
     9721 gctatcccta cggctcgacg ctcgaagcca tgggcgactt gcaggggcgc ggcatctgga
     9781 ccttcaagca ggacggtctg ctcgttgaca tcacctacga ttggaaggtg cgtgcgcgca
     9841 agcccatcgt gcgcgccctc tcgctactgc tgcgccccct tctcgcctcc aatcatcaat
     9901 acgccatgcg gcgcggcgaa gagagcctca aactcgaact cgcgcgacgg cgtgcgcgca
     9961 cccccgaaga ggccgcacgc atcccgccgc caccggcgaa ataagggatg agggcggagg
    10021 gatgagggat gaataagaaa ctactcaact ctcttgagtg cttttcttat tcatccctca
    10081 tccctcatcc ttcatccctt aagttttccc gtgagcatca aggcgaccat cttgtagtcg
    10141 gcggcgaaag accagagcgg gtgtttgaag gtggcggggc ggttgtgttc gatcaggaag
    10201 tggctcgccc acgccgccgc gtagccggtg gcaagtgcgg cgggcagcca acgccagcgc
    10261 cccgtggcga cgagggcgac cgctaaagtc gtactcgtga tcgtgcccgc cgcgtgcagc
    10321 aggcgcgtgc ccgtgtggct gtgctcggcc agatagtaag gccagaagtc tgcgaaagat
    10381 ttcaactccg gttctgtgag cgtttcttcc ctcaacattc tcttgcacct gtgttttgag
    10441 attcttactt ttccagccgg aagctcattc ctcgcccgtg gaaaatagct gttgatattg
    10501 ttcggggcgc gtgggttcga tgcgcgtgag ttggaggttc tcttcgacgt gcgcgcggcg
    10561 gctcatgccg acgagggcgg tggtgatgcc gggcgtggag cgcgtgaact ggatcgccgt
    10621 gagcgcgtcg gaggcgagcg agccgagcgg ttcgcgtatc tccggcgaca gatcgcgcgc
    10681 cactttgccc tgcaagatgg atgcgctcgc caccaccgtc acttcgagcg cgcgtgccgc
    10741 ttcgagcacg gaaaccggcg cgccctgaaa agtttggttc gacacggtga gagcttccgg
    10801 catcgcgagg ttgaacggca gttgaatgaa gcggaaatga tgctgctcgc cgccgacctc
    10861 ccgcgcgagt tccaacatgc gttcgagcga atgatagccc tgcgcgccgg gcgcgacgcg
    10921 aaagccgttc cacgtcgcca cgccgtagtt ctttatcttg ccttccgcca cgcttcgctc
    10981 caactgctcg aaagccgcgc gcagacgcgt ctcgaattcg gcgcgggaga cggagccgag
    11041 ttgtgattcg ggattgtgta tgtaatagac atcaatcgtg gcgaggttca tattgcgcag
    11101 cgattgatcg atctgatgcg cgagatattt cggcgtcatg caatggctgc cgcccgcgat
    11161 ctccgagaaa cttgcgatgc ccgttttgat gaaggtcgtt tcgatgtagc ggcgcacgcc
    11221 ttcctgcccg ttgggcgggt cgccgtcgaa gggaatgtac ccgcccttcg tgcagacgac
    11281 gatctcgtcg cgcgctaccg tggcggcatc gtcgctttcg ataagcgcgt ttaaggctgc
    11341 gccgattgat cgttcgctgc gctggaaacg atagttggcc gccgtgtcga tgacgtttgc
    11401 gccgagttcg acggcgcacg tgacggcttc cgtgtaacgc gcgtcggtcg cggcgtctgg
    11461 cttgccgaga taggtgccga tgccgagcga agagagccag aggttctgtt cgagccggaa
    11521 gtggttcggc gtaatcgccg cctgcatacg ctctcgatag cgcgaggttc cctcaatcgt
    11581 ggcgtgacct gcgatggaca tggttcaaac ctccggtttg aaagtgacga gtgacaagtg
    11641 acgagtttaa gacaggtgaa tcgtgaatcg taaatcgtaa atagagaaag actgtcttcg
    11701 tctattcacg attcacggct ttcactcatc actcatcact catcactcat tactcatcac
    11761 ttttctttct cgtcactgtt ataggcggag actataagcg ttgcgggcgc ggcgcggcaa
    11821 gaatatttca gggaagcgcg ggcggtgtgc tagggtctta acttccttag cgggttctaa
    11881 aatatgaata gagcattcaa cgcagagacg cacgaagacg cgggcggcgc gtcgctcgac
    11941 atcacggacg aagcgttgcg cgaactgagt agtcgcgccg tcgagttggt gtcagactac
    12001 tttcagagcg tctccgcgtc gccagtcttg acccatcagg gcgcgagcgt tctcgacgcg
    12061 caattcgacg cgccgctccc cgccgcaccg gacacgctcg acgaactgtt tgccgattgc
    12121 cgtcgcatct tcgagggcag ccgccacagc gggcacccgc gcttcttcgg ctacgtcgct
    12181 tcgcccgcca cgcccgtcgg cgcgttcgca gacctcatcg cctccgcgct caaccagaac
    12241 gtccccgcat ggcgttccgc tccgtcggcg acgcacgttg aacagacggt cgtgcgttgg
    12301 ctcgccgcgc tcgcgggcta cagcgcggag ggcgcgagcg ggttgctgac gagcggcggt
    12361 tcgatggcaa acctgaacgc gctctacgtc gcgcatcgaa gccaagcccg cgcgaagttt
    12421 gacggcggag atgatgcggc gaagagaagc ggcggcgatg tctcgcaaaa gggtctgtgg
    12481 cagacggcgc gaccggcgac gatctatgtc tccgatcaag cgcacatgtc tattgcgaaa
    12541 gcggcggaca ttttagggtt cgggcgcgaa caggtgcgcg tcgtagagac ggacgagaac
    12601 tttcgcatgg acgtgcgacg cctgcgcgcg gcggttgagc gcgacgcgcg cgacaacttc
    12661 gagccgtgct gcgtggtggc gagcgcgggc acggtcaaca cgggcgcgat tgacccgctc
    12721 gcagagatcg cagagttggc gcgcgaacat gatctctggt ttcacgtcga tggcgcatac
    12781 ggcgcgctcg cggcagcaga cgggacgaag cgtgagagtc tagccggact cgccagagca
    12841 gattccgtcg cgctcgaccc gcacaagtgg ctctacacgc ccgtcgattg cggctgcctg
    12901 ctctttcgcg atccggcgcg tgtgcgcgcc gcctgggctg ggagcgaggc cgattacatc
    12961 aaaatttttg aagagacgga acgcgagtcg ttcgctttct tcgactacgg catggagttg
    13021 tcgcgccgct tccgcgcgtt gaaaatctgg atgctgctgc gttattacgg cgcgcacagg
    13081 atcgccgccg ccatcgcgca cgacaatgcg ctcgcggcct acttcggcgc acgcgtcgaa
    13141 gcggctgagg acttcgaact gctcgcgccc gtcgaactga gcatctgctg ttttcgttat
    13201 gtgccgcccg acatgcggcg tcggctggcg actgcggatg aaaccgtgcg cgcgggcatc
    13261 gaagcggagt tggacgagtt gaacgagcgc atcatgcgcg cggtgcagcg cggcgggcgg
    13321 gcttacttgt cgaacgcctc cctgcgcggc aggttcgccc tgcgcgcctg catcatcaac
    13381 ttccgcacga cgcgacgcga catcgaagtc acgcttgaga tcgtccgcga ggcggcgcgc
    13441 gaagggtgaa aatctctttt gagttttaga gttaatgaac agggacggtg aaataaactg
    13501 agcaagccag cagagacgga agcaggagcg acgggcaatc tttttgaacg cgaactgagc
    13561 gtggcgatct cgctcgcacg cgcggcgggc gcggctgcgc tcgcacacta cggcgggaag
    13621 ctccacatcg tcaacaagag cgaaaacgaa gaccccgtga ctgaggccga ccacgcggcc
    13681 aacgacgtga tcgtgagcgg attgcaacgc gaatttcccg ccgacggcct gctctccgaa
    13741 gagtcgctcg acactgtgcg ccgtctgaat ctcgcgcgcg tctggatcgt cgatccgctc
    13801 gacggcacgc agggcttcat tgacggcaac ggcgacttcg cagtgcagat cggactcgcg
    13861 ctcgaaggcg agagcgtgct cggcgtcgtc tatcaacccg tgacgggcgt gctgcactac
    13921 gcggcgcgca gcgcgggcgc gtgggtcacg aggccggagc agcagacgcc ggaacgcctg
    13981 cgcgtctccg acgagacgga accgtcgcgc atgcgtctgg cggcgagtcg taatcatcac
    14041 agcccgcgca tggccgaggt gatgcgcagg ctcggcctca gcgaagagat caggcgcggc
    14101 tcggtcggca tcaagacggg gctgatcgcc gagcgtcaat gcgatctcta cgtgcacctc
    14161 tcttcgcgca cgaagcactg ggacacctgc gcccccgaag cgatcctccg cgaagccggt
    14221 ggacgcctga cagacctctg gggcgaaccg tttctctaca acacggagga cgtgcagaac
    14281 cgcaacggcc tcgtcgccgc gagcggcgcg gcgcacccgc tcatcatcga acgcctcgcc
    14341 ccgctcctgc gcgagttcgg gcgcacgcgc gtctcataag ttaaaaccgg aggcgcgaag
    14401 gattgaaggt tgattccgca gtcccgaaca catctctcga tccgaacgcc gaaataattt
    14461 tttgacagtt gatcgtccgc gcgttagcat ctccggcgcg acaacaccaa cgtcggcacg
    14521 tattgtcaca cggcgtcctc actcgcgctg cgccgcgtca gaatttatcg tgaaccttcg
    14581 cgccaaactc ttaatcgtct ttatcgtcgg cagcctcgca cccgtgctgc tcgtcggcgg
    14641 cctcttctat cgcaacagcg tcggcgtcgt cgaaggagtg ctgcgcgaag aggtggaagc
    14701 gaagacggcg cgcattgccg ctcacgtcga ggcagccctg cgccaacgcg agttcgacct
    14761 cgattatctg gcgcaggaca gcgcgatgcg cgaatacgcc cgtagctctg ctgcgccccg
    14821 gcctcgggca ggcgcaaaaa acgatgcgcg caccggagcg ggagccacgg tcgagtcgcc
    14881 cgcgagcctg tcgccggaag cgcaagacgc catctttgat ttcctctctc gttatcgaga
    14941 ttactattcg gcgatcacgc tgatcagcgc gccggatgag ggacgcgcgc cgatccgtat
    15001 cgagtcgccc acgggcgcag acttgaatcc gcgtgtgaag acgagtgacc tgttgcccgg
    15061 ccaaatgcgt ctcgacgagc gcgtctggac gagcggcgag cgacgctccc tgcgctcaag
    15121 cgtgaagaat gcggcgaccg gcacgccctg catcgcctac accctgccgg tcttctccgt
    15181 ggagaatgaa ctcacgccgc gcctcgcact cgtcgccgaa ctccgactcg acatgctgtt
    15241 gcaggaggcc gggagcgatt taaacgctgc tgccgccaca tcctcggctc ccgctttttc
    15301 cgcgcgcgtg agcgaggcgg ggaaatactc gcgcctgctg gtggtcttag acgatgcggg
    15361 agagatcgtc tatcacacga accacgcgtt caaataccag cccgtgagca acgcgatgcc
    15421 ttacttcgac gcaaccgcgc gggcaatgca atcgggcggc acgggaacga gcttctacac
    15481 cgtgcccgca gacggcgcgc gctggctcgc ttcctacaaa cctgtcgccg ggagcaatct
    15541 ctccgtggcg gtcgccggaa attacacgcg cgccgtgtcg agcctcaacc gcaacgcgct
    15601 cctggtgctc agtctctccc tgctcatcat ggctgccgcc gcgctgctcc tgttcgccgt
    15661 cgccacgcgc gccgcgcttc gcatcaggcg agtcaccgaa gcggctgccg ctatcgccac
    15721 caccggcgac ctcaaccagc gcatcgaagt gcaggccaac gacgagacgc acgcgctcgc
    15781 cgaaggcttc aaccggatga gcgaccgcct gcgcgaattc atcgcacgcg aagccgagat
    15841 gcgacagttt caatctttcg tccgcatctc cgcgatgctc acgcacgatc ttaaaaactc
    15901 catcaccgga ctctccatgc ttgtgctcaa catggaacgg cagtttcatc gcgcggagtt
    15961 tcgcgaagac gccatctctt cgctcaagca ggcgacggaa aaactcggcg gcctcgtcgc
    16021 gcgtttgagc gagccggtga agtctctgag cggcgaatac aggcgcacgc cgcagcccgc
    16081 cgatctcgtc cccgtcatcc gccgcgtgat ggccgccagc gccgaaccct ccgccgcctt
    16141 ctacgatatc cagacggaac tgccccccac gctcttcgct ctgattgacg ccgaccgcat
    16201 cgagcgcgtc gtggaaaatc tggtcgtcaa cgcgctcgaa gcgatggggg ggcggggcgg
    16261 gcggctcacc gtcaccgccg gggaggaatc ggaagggctg gtcttcttca gcgtcgagga
    16321 caccggaccg gggatgaccg aagacttcat gcgcacgcgc ctgtttcgcg cctttgccac
    16381 gacgaagaaa cgcggcatcg gcctcggcct ctatacctgc cgcgagattg tcgaatcgca
    16441 tggcggacga ctagaagtta aatccaagct aggctctggc acgcgctttc gcgtcgtgct
    16501 accatccatc tcgctcatgg cgtcacatgc ccgttcgttc gataaacccc cacgaaacaa
    16561 ggaggcgaag acgacaaaga cgggttcggc gcgttgagcg cgtcccctta gcttccgcga
    16621 gttcattaaa cattatctag ggtggctgga aattgtccgg cctgccctaa ccgggtggcg
    16681 agcgcggatt tcaggccgga gtctttcaaa cctcgacgct aacgcaccca ctgccgcatg
    16741 agcgaacaca cgcccgaaca gacacccgac aaagcgaccg tactcgtcgt tgacgacgat
    16801 caagttttgc ggcgtcaact cttctgggcg ctcgactcgg agcatcatgt gctcgaagcc
    16861 gactcgcgca tcgcggccat cgcgcagttg cagagcgggc gcgtcggggt cgtcttgtgc
    16921 gacctgcacc tgccgccgga tctggacggc atcagcgaag gtctggcgat catcgaagcg
    16981 gcgcgcgccg cccgtccgag cattcccgtc atcgtcatca ccggcagcaa ctcgcgccag
    17041 accgcgctcg aagccatccg ttgcggcgca tacggttttt ttgaaaaacc tttcaacgaa
    17101 gatgaagtca cgcacatcgt ccggcaagcg gcgcgcgtgc gccatctcga agaagagaat
    17161 ctacacctgc gcgcctcgct cggctcgttt cgcggtttcg ggcgactgct gggaagttcg
    17221 cccgcgctgg aacgcatcat gaagcaggcg cgcgccgtcg cctcgacgag cgcgaccgtg
    17281 ctgctcacgg gcgaaaacgg cacgggcaag gagatgctcg cacgcgccat ccatgaagag
    17341 agcgcgcgcg tcgatcagcc tttcatcgcc gtgagttgcg cggcgttgcc ggagtccctg
    17401 atcgagtcgg aactgttcgg ccatgagaaa ggcgcgttca ccggcgcggt cgcagccaag
    17461 aagggacgct tcgagttggc cgacggcggc acgctctttc tcgacgagat cggagaactc
    17521 agtcaggccg tgcaggtgaa actgctgcgc gtcttgcagg agcgaaactt cgaacgcctc
    17581 ggcagcacca agactcagac ggtcgatatt cgactcatcg ccgccagcaa tcgcgatctc
    17641 gaacaggaag tcgaagaggg acgcttccga cgcgatctct tctatcgtct gaacgtcgtc
    17701 ccgctgcaac ttccggcttt gcgcgaacgg cgcgaagacc tccccatcct cgccgcgcac
    17761 ttcgcctccc gcgccgccga aaaacatgga cgcccgacgc ccgcgctcga acccgccctg
    17821 ctcgaagcct tgcaggagta cgactggccg ggcaacgtcc gcgaactcga aaatttgatg
    17881 gagcggctca tcgtcttgac tacgggcacg aaactcggca tcgagtttct tcccgaaagg
    17941 atgctccacc tgcgccgccc cgtcgcgccg gaagcaggcc ggaacggcga gaacctctac
    18001 gatactaccc tcgaaggcgc agtcctcgcc ctcaaacgcc aactcgtcat caacgccttg
    18061 caggccgaaa gcggcaacaa agccgccgcc gccaaacgcc tcgccatcag ccgctcctac
    18121 ctccaccggc tcgtcagcga attaaacatc cccacgtgaa gaaacgtcga acgccgaact
    18181 gaaaacaagt cgtctttgat tcatcgttcc gcgttcatcc ttcatcgttt aatttgtcct
    18241 cttctgtgta cacctcgaaa ttccactgtc cacgttagtt cacacctgtc gctcgccggg
    18301 acagggggcg tgcgtgtgat tgctgcgaaa tgggcggttt tcgttatcag cagcgcatgg
    18361 cacgcgcgtt gcgaagagta tcaagcgtag agacaggaag ttaattgaga agttcgagac
    18421 tgtgaagaag agcgtatgcc ggacgaaacg ctcggagcga agattgcgat ctcgctctca
    18481 ggcgggtacg gcgaaaaggg gcgggacgtt gaaaccaaaa cttctgatcg ttgacgacga
    18541 cagcgccatc cgccagcaac tcttctggac gctctgcgac gagtatgacg tgatcatggc
    18601 gagcgatttt ccttccgcca tgcgccacgc gacgagttct gagccggtca tctcgctcct
    18661 tgatttgcac atgccgcccg tgctcgattc gccggaagtc ggcctgcgcc tcctcgatta
    18721 catcaaagag cagtgcccgt cttcgaaggt ctatatcgtg agttccgaag agagcattga
    18781 gacgaagaaa gcctgtttca agcagggcgc agacggcttc ctcagtaagc cgctcgatgt
    18841 cgaacagctg ctcacgaccg tgcgccattc gacgctcgcc gcgcgactta aagcggcctg
    18901 aaaaattttc tgaaatgata tgcgctgtga acgcatatta ggttaggcgg tgagtttttt
    18961 gaaagccatt ctcaaacgga ctgacgcgga cggcgcgcgc gagttgacgc ccgccgagat
    19021 acgcggggtg ctgctcaagt tagcgcgcaa gaagatttcc gaacgggtag cacgcccggc
    19081 catcgacgcg ctcaacaaga tcatgtgcga cgccttagcc gcctcgcagc ctttacgcgc
    19141 gacgcaaggc aagcacgaag gttgataaat tagactcttc ctcacacacg agggcgcggc
    19201 ggttccccct cccccaacgc cgccgcgccg gaaatgttct ttgagagatt ttacgagttc
    19261 ggctctcgtt cccttcacga gagccgtagt gctccgcgca catacggtat cccttctgcc
    19321 gccgcccgga gccgaagccc ccgcttccaa atttgactcc ttcccagagt cgcgggggct
    19381 tcactttttt taagcgacag gtaacgagtg acaagggaca agttaaaaca aaacacttgt
    19441 ctttaactcg tcacttgtca ctcgtcactt ctcactctct ttaagtataa tagtcacatg
    19501 aaagcgtctg catcgtgacc ttccggttca tccgggagcc gtgttgcgct atcgctcgtg
    19561 actattatga aatttcttca tctcgcagac atacatctcg gctgccgtcg ctacaacgtg
    19621 gaggaacgca cgaccgactt cttccgcgcc tggcgcgacg tgatcgaaca acacgccctc
    19681 ccgaatgcgg ttgacttcgt gctcatctgc ggcgacttct tcaactcgcg caaagttgac
    19741 ccgcaggcga tgaaccacgc catcatcggc ctgcaaaggt tgaaagaggc gggcattccc
    19801 gtcgtcgcca tcgaaggcaa tcacgaccag cacgagtccg atgcagttaa tcgcttcagt
    19861 tggatgcgct cgctctcgca gtggggcttc ctcacgctgc tcgaaccgga cacgcgcgac
    19921 ggcttcaacc tcactccctg gaacgaagag gaacgcagcg gttcttacat agacattgcg
    19981 ggcgcgcgca tcttcggcac gcactggttc ggcacttcca ccaacgccgc catccctctg
    20041 ctcacggatg cgctcaggcg tgcgcgcggc gaaaacctgt tcaatatcct gatgctgcac
    20101 acggacgtgg aagggcaact caaccgtccg aacatccccg cgctctctat ctcccgcatg
    20161 aaggaactgc gcacgctcgt ggattacgtc gcgctcggcc acacgcacaa gcgtttcgag
    20221 atcgaggact gggctttcaa tcccggctcg ctcgaagcct gcaccattga cgagttcaaa
    20281 gaagaacgcg gcctttatct cgtcgaagtt gacgaagcgc accgcgtcac ggcgcaccac
    20341 tcgcgcgact acacgcagcg accgtttcaa cgcctcaatt tcgacgtgtc cggcgtcgcg
    20401 gatgccgaag ccgtccacga gggcgtcctc gaagtgctac gccgcgaagc gcgcacgcac
    20461 gaccccgaac gcgacgagac gcctgcgccc attatcgaga tcaacctgcg cggccatctc
    20521 ggcttcaaaa attcgctgct cgacattccg cgcatgcgcg aagaagcgcg tacgctgacc
    20581 ggcgcgctcc acatcatgat cagcaaccgg agcgtgcccg tcgagtacgc cgtcgccgca
    20641 gggcttgact ccgacgccac acggcaggtg cgcgaacgtc gcatcatcga agatttgatc
    20701 acacgcgaca tacgctttcg tcatcgcgcg cacgacatgg ccgcgctcac gctcgaagcc
    20761 aaacggctcg ccctcggcga cgagtcgccc gagaagattc tcgaactcat cgagcaacaa
    20821 ctctacacgg acgcggaaca accaaacggc gcatcttcaa acgcgatgag cgacgccgca
    20881 gcaggcgata cggcgggcac gcctatcgcg acagacgaca cggaactcgt cgcctccgcc
    20941 agcgcattgc aggcaattga acgaaacgcc atgaaagccg tgacgagtga caagtgacaa
    21001 gttaaagcag aagaaaaact gtctttaaaa cttgtcttgt tcccactcgt tacctgtcac
    21061 tgttctaata tgccagattc tttcgagaga gtgcgcggcg tgagcgagtt gccgctgttc
    21121 cctttgccgt tggtgctgtt tcccggcgtg ccgatgccgc tccacatctt cgaggagcgt
    21181 tatcgccgga tgctcgcaga cgtgcgcgcg ggcaacaatt tgttcggcct ctcttacttc
    21241 gatttgaaca cggcgaccaa cacgcgcccc gcacccggcc atgtcggctg tgcggcggaa
    21301 atagtcgaag cgcaactcct gcccgacggg cgttcaaata ttttgacggt cggcctgata
    21361 cgttatcgcc tcgtcgagta cgtcgaactg ggtgagccgt atctggttgg ccgggtggag
    21421 ttttttgaag acgagccgga agacgaagcg ttgctgctgg aacgcgcgca cgaggtcgcc
    21481 gaactcttca tgcggatcgc tcgcgccgtg cgcaacttga acgacgaccg cgccggtctg
    21541 cccgaactcc ccgacgccga accggaacgc ctctccttcc tcgtcgccgc cgcgatggaa
    21601 ctcgacgcag aggccaagca aggtctgctc gaactgcgct ccggggcgca gcggctcgaa
    21661 cgcatcggca atctgctctc gcaagtcgta ggcaactacg aagaacgcgc gcgcacgcac
    21721 aaactcgcca gaggcaacgg ccacggcggc aatcatctga acctcgacca atgagttctc
    21781 ccggcaacga acccacacga cagacgaacg actcgccgca gacgatccgc gtgcgcctgc
    21841 tctatttcgg cgcggcgcgc gaagaggttg gccgcgaaga agagcaactc gaagttacct
    21901 cgcccgccac ctccgcgagc gtgctggcgg aagtcctgaa cgcgcatccc gcactgcgcc
    21961 gcttcggacg ctctttgctc gtcgcggtca acgaggagta cgcgggagcg tctgcgcgcg
    22021 tggaggcggg cgacgaggtc gccatctttc cgcccgtcag cggcggcgcg ggcggcaggg
    22081 aaggagaggg cgtgggcggc ggcgtcgaag cgggcgagcg ggaggcgtgc gcggcggaag
    22141 atttctttga actcactact gacccgatca acgtcggcgc agtcgcgcga cgcgtcgtgc
    22201 cgcccgcttg cggcgcgacc gtcacgctcg acggctacgt gcgccggttc acgcgcgggc
    22261 gcgaggcgac gctctatctc atctacgaag cctacgcccc gatggccttg agcgagatgc
    22321 gtaggctcgg gcggcaaatc cacgagagat tcgaggtggc gcatgtcggc atcgtgcatc
    22381 gcacggggcg tctggaaatc ggcgaaacct cggtcgtcat ctcggtgagc gcgccgcatc
    22441 gccgcgccgc ctttgaagcc tgcgagtggg cgatacgcga actcaaacgc accgtcccga
    22501 tttggaaaaa ggagtttttt gccgggggcg aagtctggat cgaaggtgaa ggcgcgccgg
    22561 ttaattgaag atgaagatga aaaaattaga cggactgctc gcacgcaatc gcgcgtgggc
    22621 gaaaaaaatg acgacggctg accccgattt cttcaagcgt ctctcgcaac aacagacgcc
    22681 cgagtatctc tggatcggct gttcggatag ccgcgtctcc gcaaacgaga tcatcggcct
    22741 cctgccgggt gaaatattcg tccatcgcaa cgtggcgaac gtcgtggtgc acaccgacct
    22801 caattgtctc tccgtgattc aatacgccgt ggaggtgctc aaggtgaagc acatcatcgt
    22861 gtgcgggcat tacggctgcg gcggcgtgaa ggccgcgctc gataacggca agcacggcct
    22921 gatcgacaac tggctccggc acgtgcagga cgtgcagcgc gatcaccaga aagagctgcg
    22981 cgcgattgcc ggtgatgcgg cgcgggtcg
//
LOCUS       DASWNS010000232         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_60832, whole genome shotgun sequence.
ACCESSION   DASWNS010000232 DASWNS010000000
VERSION     DASWNS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60832"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VGB76_08025"
     CDS             complement(<1..330)
                     /locus_tag="VGB76_08025"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HEY0098887.1"
                     /translation="MDAVDETIIYLSRTKILLLLLGAFAFVAAGIWMFSLGDASIQSQ
                     RRFNEPLYVRGLGLAAILFFGSCGLYALKKLFDRKPALVFNSSGIVDNASSVAAGLIP
                     WSDVVSAG"
     gene            complement(811..1236)
                     /locus_tag="VGB76_08030"
     CDS             complement(811..1236)
                     /locus_tag="VGB76_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098888.1"
                     /translation="MTTSPAQERRGSARLKILLVLLGVFILGGVTGASLDSLYRLRGR
                     SHEGRQGRGHRGRDDMFEKMRRDLNLSEQQATEIRAILDQTRNDYRALRTEVRPRYDA
                     VRQQARTRIRALLTPEQQKRFDERAAERDARRGDNDDEK"
     gene            complement(1233..1679)
                     /locus_tag="VGB76_08035"
     CDS             complement(1233..1679)
                     /locus_tag="VGB76_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098889.1"
                     /translation="MIGKRKKDAVAVEELERLGRAVVRASAANEAEAEAAASSPFLYT
                     RLRARIDAEQEARSAGGAETWRTLLGVVWRSVPAMALVALLTFILFWTSNFGNTSLSP
                     GVLSDEALLDARDAGIESVVFAERRPLSSDDVLATILQEDEREGSR"
     gene            complement(1755..>2344)
                     /locus_tag="VGB76_08040"
     CDS             complement(1755..>2344)
                     /locus_tag="VGB76_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0098890.1"
                     /translation="DSNDGLSDVELVSRATAAGGDEAAFEELFNRHRRRVALIAGRFF
                     RQREQIEEVVQESFMKAYFALPDFTNQQENSFASWIARIAFNTCYDELRRLKRRPESA
                     FGEISEEEAMWLKTQLRATGAGDNVEANAIARDLADKLLARLSPEDRLLLVMLDVEGL
                     SVAEIGETQGWSISKVKVRAHRARASLRRVLGRFL"
BASE COUNT          436 a          712 c          719 g          477 t
ORIGIN      
        1 ccccgcactt acaacgtcag accacgggat gagtcccgcc gcgacgctgc ttgcgttatc
       61 aacaatccct gagctgttaa ataccagagc gggctttctg tcgaatagct ttttgagcgc
      121 gtatagcccg cagcttccga aaaataggat cgccgcaagt ccgaggccgc gcacatacag
      181 cggctcgtta aaacggcgtt gagactgaat gctcgcgtcg ccgagggaga acatccagat
      241 tcctgctgcc acgaaggcga atgcgccgag cagaagcagc aggattttcg ttctgctcaa
      301 gtagattatc gtttcgtcaa cggcatccat atcgtgttta ctatgcttga tactcgatta
      361 agctgccgcc cgaaggcata ggtggaaggc ggtcagccgc tatcgaggcg gcgggttgtt
      421 acgctgcgta ggtcgaaggt taaatctgcg agaagataat caagggcttg tctccaacgg
      481 taattcaaat ggaacgtaag accctgccca tttgagagct attcacagaa accggcttta
      541 gtcatgatcg tcgtgacaaa aagcctgcca ttagtcggca gacggcgaga gggcagaaca
      601 atactggcgc tgactgtggc tgtcaaatat ttccgggcag aggatagagc agcctgactg
      661 cgggcatcac gggagtgatc ggcctgtgtg ttgtgacaaa ctgcggagtc tcggtttgac
      721 aagcggaggg cgactttctt tgcgggtcgg gtaaatcgag ggcgcgtcta cggtaattcc
      781 agggtaatta tcgcaggacg cgcccacgct ctatttctcg tcgtcgttgt cgccgcgccg
      841 tgcgtcgcgt tcggcggcgc gttcgtcgaa gcgtttttgt tgttcgggcg tgagcagggc
      901 gcggatgcgc gtgcgcgcct gctggcggac ggcgtcgtag cgcgggcgta cttctgtacg
      961 cagggcgcgg tagtcgttgc gtgtctggtc gaggatggcg cgaatttccg tcgcctgctg
     1021 ttcgctcagg ttgaggtcgc ggcgcatctt ctcgaacatg tcgtcgcggc cacgatgccc
     1081 acggccttga cgcccttcgt gcgagcgtcc gcgcaggcga tagagactat cgagcgatgc
     1141 gcccgtcacg ccgccgagga tgaagacgcc gaggagcacc agcaggattt tgagccgcgc
     1201 gctgccgcgc cgctcctgcg cgggcgaggt ggtcatcgag aaccctccct ctcgtcttcc
     1261 tgtagaatcg tcgccagcac gtcgtcgctt gagagtggcc gtcgctccgc gaacacgacg
     1321 ctctcgattc cggcgtcgcg cgcgtccagc aacgcttcgt cgctgagcac gccgggcgat
     1381 aaggaggtgt tgccgaagtt agaagtccag aacaggatga acgtgaggag ggcgacgagc
     1441 gccatcgcgg gcacgctccg ccagacgacg ccgagcagcg tgcgccacgt ctccgcgccg
     1501 cccgcgctcc gcgcttcctg ctcggcatcg atgcgcgcgc gcagccgcgt gtagagaaac
     1561 ggcgaagagg ccgccgcttc cgcctccgct tcgttggccg ccgaggcgcg cacgacggca
     1621 cgcccgaggc gttcgagttc ttccaccgct acagcgtcct ttttccgttt gccgatcatt
     1681 tcagttttac ctgttttccg ccgccgcatc tcacagcagc tacgttgttg aaaacgcgcg
     1741 cccgcgcccg ccggttacaa gaatcgtccc agcacgcggc gcaaactcgc ccgcgcacga
     1801 tgcgcacgca ctttgacctt cgagattgac caaccttgtg tctcgccgat ctcggcgacc
     1861 gacaatcctt cgacatcgag catgacgagc aacaggcgat cttcggggct gagacgcgcg
     1921 agcagcttgt ctgccaggtc gcgcgcgatt gcgttggcct ccacattgtc gcccgccccc
     1981 gtggcgcgca gttgcgtctt caaccacatg gcctcttctt ccgagatctc gccgaacgcg
     2041 ctctccggtc gtcgcttcaa acgccgcaac tcgtcgtaac aggtgttaaa ggcgatgcgc
     2101 gcgatccacg aggcgaagga gttttcctgt tgattggtga agtccggcag cgcgaaatag
     2161 gctttcataa agctctcttg cacgacctct tcgatctgtt cgcgctggcg aaagaagcga
     2221 ccggcgatga gcgcgacacg acgccggtgg cggttgaaga gttcctcgaa cgccgcctcg
     2281 tcgccccctg cggcggttgc gcgcgagaca agttcgacat ccgacaatcc atcgttcgag
     2341 tccg
//
LOCUS       DASWNS010000233        15253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_243013, whole genome shotgun sequence.
ACCESSION   DASWNS010000233 DASWNS010000000
VERSION     DASWNS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15253
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243013"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..642)
                     /gene="ndhC"
                     /locus_tag="VGB76_08045"
     CDS             complement(163..642)
                     /gene="ndhC"
                     /locus_tag="VGB76_08045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY0098891.1"
                     /translation="MRNFDLKDYLPILLMFLVAGGFAGGNILLSQLVGQRKQTRTKLM
                     PYECGKDPVGSARERFSVKFYLIAMIFILFDIELIFLMPWAVVFKSLAAQGAAMRNLI
                     FIEMMLFVVLLLVGYLYVLKKGLFDWNERARAEAEAEARALALKEGRRAELEHRAAA"
     gene            898..1830
                     /locus_tag="VGB76_08050"
     CDS             898..1830
                     /locus_tag="VGB76_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098892.1"
                     /translation="MITGFNTDIEFGGVVYHVQTEDKGLDTPLILTLVYVGGAILASK
                     RSQYHDLIRAGFDPLVLSERLQRQHTLICAAVKAGRIEDLKRLTPRGEAEAPIAPTPI
                     AKIKPVARAVPPPAVVETETPKIVETKAPPVETKPAKVVETITQRVSESATSGDILAL
                     DDEPSEIVPVISEPVRSEPLRSEPRRVEPPEPVRLVGSQNDDFIQVEAEDALHLNLLE
                     ERDFHAGEVVTLRIRVSRGGGDVRVAAADVPVTVKILGTEFRPLILTTRSDRDGVATI
                     HAWLPRFTKGRAAVLVRAAIDGFSAELRRVILQP"
     gene            1860..2072
                     /gene="thiS"
                     /locus_tag="VGB76_08055"
     CDS             1860..2072
                     /gene="thiS"
                     /locus_tag="VGB76_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY0098893.1"
                     /translation="MRVQVNGEIRELPEGTTLGELVRELSLAPERLAIELNREVVRRA
                     AWPETEIREGDAVEIVHFVGGGSGEV"
     gene            2234..2953
                     /locus_tag="VGB76_08060"
     CDS             2234..2953
                     /locus_tag="VGB76_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098894.1"
                     /translation="MIALFIIAFVLLAATAYTFYRRQHASPDYRAELHSRHASPRSLF
                     DHKEADTENARLDAAQADREIEERNASLLKRAAAGDILTLAEAAHANDPALYERVLHN
                     LLEQAGATEAGLRSLASFIANDSGLRGSPDLAAAYTKLWQQQPDRQTTAQLLHLAALS
                     DDASTFERAIVAACDLLRSGRLGKVRPEELSALIESEYWVLSSEARRTGAGFVLKQRM
                     TSLREELLAGNRKTESPKVDS"
     gene            3082..5286
                     /locus_tag="VGB76_08065"
     CDS             3082..5286
                     /locus_tag="VGB76_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0098895.1"
                     /translation="MPYLIVLGIVVLLLLILLSKMFVNVGGREIAIKERRYLGAKMPP
                     GRVVATEGEVGIQADVLKPGLHLIKWPFESVVRKVPLIEVGAEELGIIEAVDGEPMPP
                     GRIFAPDRAENAHNNFQDPIAFIKQGGVKGIQLRTLPPGLWPIHPYLFRVSIAKATVI
                     PPGKVGVVTAADGDQLDAGRLHGKATPGHRNYQDAEQFIASGGQKGPQVEILTPGTYR
                     ILTQSISIEDGSETKHGLFFVRHYDATIINENQIGLVEALDGAPLDPRDYVATPVQGH
                     DNFQDCNEFITGGGQRGPQKDILLPGTYYINPLVFKVIPESAKEVKPGEVAVIVSNIG
                     KDPGEEVRKVMAAKVRERMEREEHEHVEQAAARLDKLGDENRNLAEIQEDLLSGDPAD
                     RRLDEGAHEAYVVPEGYRGIQETVVGPGRYYVNTLAITPIVIPTTNQTVEWTAGEVNQ
                     SFNPFEVISKDGFTMQLEVRVVFRVKPEDAPFMVAKIGSIDKLIQNVMHPLIDSIFRN
                     QASESSAMAYLQNRHEEQERAEARVRAHLLKYHVDVVNVLICHIHLPAELMKTQTEKI
                     LAEQRQNMYDAQREAEEKRIQLARISAQADNQKDLMAATVGVDIAGKRSEQRKAEADG
                     EAHYILATGKAEAEKVRLMGEAQGVAYHEQVNALGAQGVALVETLRVIGEKGVRITPD
                     VMASGGNGDGAGNLGTLLLLNLFRDRLPGGAGENGAKLASGDGAGNGAKLKA"
     gene            5410..6201
                     /locus_tag="VGB76_08070"
     CDS             5410..6201
                     /locus_tag="VGB76_08070"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY0098896.1"
                     /translation="MSTTLITQTETFEIGGRAFHSRLIIGTGKYRSFEEMKAAHRASG
                     AEMVTVAVRRVPLDRSSESFLDHLDPALAILPNTAGCYTAEEAIRTARLAREALNTDL
                     IKLEVIGDQTTLFPDNEQTLEAARVLVKEGFTVLPYFSDDLIMAKKLLDAGCAAVMPL
                     AAPIGSGLGVQNPANLLIMREQLPEATIIVDAGVGTASDAAIAMELGADAVLMNTAIA
                     EARDAAQMADAMRLAIRAGRLAYLAGRMPKRLYASASSPFAGVVR"
     gene            6214..6720
                     /locus_tag="VGB76_08075"
     CDS             6214..6720
                     /locus_tag="VGB76_08075"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY0098897.1"
                     /translation="MSSDAPKPTFKSVEVNIFNQVYSLRTNREVEHIRRIAGLVDERM
                     RLISSHATAHDLAKIAIFAALNIADELSDTREHYEREFQKLLTRANESEGEPEPAVEP
                     ERFEETPLEDVQRSEERGSWFEDIFDAPESDNAKGRSERLSSKISAKLQSLRQSKTET
                     SFSIEEGD"
     gene            complement(6825..11795)
                     /locus_tag="VGB76_08080"
     CDS             complement(6825..11795)
                     /locus_tag="VGB76_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0098898.1"
                     /translation="MKSHTRLFASSLCLALALGLCVVVAGVRSQNDAPLRRITLTSER
                     ALNLNPSISGDGQHIAFESTEEIGGAGGSGTGFHTLRADIYADGATFTRIAATRAPAP
                     AISHDGSRLAFASRENLTGENSDGNSEIFLYDGERLQQLTDTSPRDAAQRIVEGNFQP
                     SISNDGTLLAFASNRDLTGANPDANFEIFVYDSATRATEQITHTSGINGATDAKLSGN
                     GARVAFVRDNGATGGVESSPQRDLLIYERAQGSTRVIAKDVDGLALTYGRATSDDGAR
                     VVYAAQTAARTTQIFLYDGRNDQLRQLTSLGSRAADVPLHPTISGDGSRIAFATRRNV
                     NGGNSDASVELYVYDLPTNRVSRVTDAPAAATAEVVSSLNEDGSRVAFNFPRLLSGTI
                     SDSSFVNHTEIYLARLDARPPFSTGLQILNGASLDREPAATRAIAPGQIASARGVNLA
                     LDAIQAKRLPGGTFPRTLGGASLYVNGRSAQLFYASPTQINFLVPAETETGAAHVVVR
                     NHDGYESRASVSSLHVAPGVFTERGDGSGAAIALDAETFLRSPFDPVDAANQPRRLTI
                     FTTGIRHAKHVTVTIGGRELDVEKILPSPDLPGLDEVHIRLSRSLAGAGTVPLLLRAD
                     GRDSNPTTLNFKGTRRAASITLEPSNASIGVGRSVRFVATVRDADGVEITNAPVAFTS
                     EDATIATIDADGSARGVRAGTATIKAVSGEVSASAQLTVFPLSLVINEILADPPEGAA
                     GDANRDGTRSSAQDEFVELVNASTRDLDLGGYQLTVRNSNNTDIVRHTFAADTILAPG
                     TSIVVFGGAQGSTFNPAHPAFGGALVLPASTGGLSLTNGGSTVKLLDPAGATIDLFTY
                     GDTNGLDADRNQSLTRAPDALGDFALHQTAHGSDGRAFSPGTRVDGTPFDITVSVARI
                     EVAPAHATIERGAQQQFTARAFDLNGNELRGVIFRWQSSDPAVATIDGDGLAQSHATG
                     EAFIEATARGVQSTPATLRVLAPQPKVTRVEITPHAASINRGGALQFTARAFDKDGRP
                     LDRTTFTWSSSDAFIATIDAAAQARGTGVGLVNITASTPDGAGGVVSDQASLEVRVPL
                     VINEIYADVSPDDPATAAVEGDANRDGVRSSVDDEFVELLNYSREALDISGLSVADAT
                     ARRYTFPAHTILAAGGALVLFGGGAPPHNDPAFGGANLFTAASLGLNDAGDTVSLKLS
                     LNSVEITITSQSYGTAAPNSPPAPADQSLTRSPDAAPGSTGGDFNPHTLAPGAATRAY
                     SPGTRADGTPFDSTPISRISILPPASTIEHGASQDFSARAYTVLDGVELEVAHVSFIW
                     DTSDAGKASVAPQTGRQTTARGLSAGNVSVRARAGGREATASLTIKPPPPVLTRLRLS
                     PAAATLNVGDSQQFTAQAFDQYDQPFPVSSITFASDETQVATIDSVRHTPGSSDASAT
                     IVARATGTARITATARDESRPVSSNTATVTVNSPPQVPAAGQVVINEALVAFAASTTQ
                     VRRDFIELYNTTDRTLDITGLVVTFRPSGSGNTPFSLTLEDASGGRFLLAPRGYFLIA
                     NAAETFGVAADFDASASGFDLNNTTGAVKIEMGGVRLDGLAYQGGATPPSAPFNAFGE
                     GAIFTFTGGATNDLIRSPNASDTNNNATDFRRNGTTSSVSPKAPNP"
     gene            complement(12082..13977)
                     /locus_tag="VGB76_08085"
     CDS             complement(12082..13977)
                     /locus_tag="VGB76_08085"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEY0098899.1"
                     /translation="MENFRDLLASDQITVFDGAMGTMLYAKGVYINRSYDELNLTNPD
                     LVREVHQEYVRAGADVIETNTFGATMHNLQQYGLEGSLRKINLTAAQLAREAACERCY
                     VAGAIGPLGLRIEPYGPTSFDEAKEMFKEQVGALLEGGVDLFVLETFSDISEIRQALR
                     AVRELCDLPIVAQMTILSDGNTSYGTTPEVFTTRLDEWGADVIGLNCGVGPADVLTAI
                     ERMRTVTNKRLAAQPNAGLPRDVQGRQFYMCSPEYMAKYAKRLIQAGVKVIGGCCGTT
                     PSHIKLISDAVRAASPRKARAALKPSQPARIEELQPADIQVVPMEERSNWARRLVNGE
                     FVTTVEVLPPKGCDAAKTLEQIRLLKTAGVNAVNIPDGPRAQTRMSAQATALLVEREI
                     GLEAVLHYCCRDRNLLGMMSDLLGAAALGLRNLLLITGDPPKMGPYPDATAVFDIDSI
                     GLTNMVNKLNHGLDLGNNPIGCPTSFCIGVGVNPGAINLEEEIKRFEYKVEAGAEYAI
                     TQPVFDVGQLRDFLKLIEHVRIPVIAGIWPLVSYRNAEFLHNEVPGVRVTPSIMERMR
                     RASETGKEAARDEGLQIARESLLEVRDLIQGVQVSAPFNNVRYALEVFEALPEFGAAR
                     ETTRAAG"
     regulatory      complement(14114..14189)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(14383..>15253)
                     /locus_tag="VGB76_08090"
     CDS             complement(14383..>15253)
                     /locus_tag="VGB76_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098900.1"
                     /translation="TFAVSLFMFIATLAGAFSASTASDPTRTANPAVAAAYEEGAAEY
                     VTVYAKDCRTPQTVFYLGDTVCITAGNFPLPLDAFNYRRLSWIAPNHTVANQALFYSD
                     PQTEYFEIPTSGALAQVGTWHAATINVHSGRRVNGKFYVRNPRLPFIDLSISMAAPEY
                     VYPGQRVRYNLSFHNPGPDFAEGVQIIDEVPSGMTFYALKQVSGPFFECSTPALGETG
                     RIVCNTKGLQVDETAQFAVYYLVNRDVREGTVFSSLSYISSYTDELHKENNVTKTETI
                     FPLPDAEGGDIDP"
BASE COUNT         2936 a         4483 c         5033 g         2801 t
ORIGIN      
        1 cgttcatcat ctcgatggcg caacaggcga ggccgaaagt cgccggccac aagctggact
       61 tacgcgccca gttgacgagt gcgtcgagct tcgtcgtcag cacttccggc agagcttctg
      121 caatcttatt ttccagaccc ataattaaaa ctcccgcatg gctcaggctg cggcgcgatg
      181 ctccagttca gccctgcgcc cttccttcaa agccagcgcg cgcgcttcgg cttccgcttc
      241 cgcccgcgcc cgctcgttcc aatcaaacag acccttcttc aacacgtaca ggtaaccgac
      301 cagcagcagc acgacgaaca acatcatctc gatgaagatc aaattacgca tcgccgcccc
      361 ctgcgccgcg agcgacttga agaccaccgc ccacggcatc aggaagatca actcgatgtc
      421 gaagaggatg aagatcatcg cgatcaagta gaacttgacc gagaagcgtt cgcgcgccga
      481 ccccacaggg tctttgccgc actcgtaagg catcaacttc gtgcgcgtct gtttgcgctg
      541 cccgacgagt tgcgagagca ggatgttgcc gcccgcgaag ccccctgcga caaggaacat
      601 cagcaggatc ggcaagtaat ctttcaggtc gaaattacgc acaccggcct cccgtcagct
      661 tgtgctgaga agagcgtaaa accgctcaaa acttagattg agaatgatgg cacaaagtca
      721 tgttagtgca aggtctaaag ggtgtcaagg cagaggcgac gtgccgcaaa agccgcgttt
      781 acaaggagat tgaccctcct cggagggcta tgttactgtt tctgtcggct ccggcctcgg
      841 acaactcttg aacaatattt tacctccaac gcgctccggc ggattcccga acttgtcgtg
      901 atcacgggct tcaatacaga catcgagttt ggcggcgtgg tctatcacgt gcagacggaa
      961 gataaggggc tggatacgcc tctgatcctc acgctcgtct atgtgggcgg ggcaattctg
     1021 gcgagcaagc gttcgcaata ccacgatctc atccgggcgg gcttcgaccc gctcgtgctc
     1081 agcgaacgcc tccaacgcca gcacacgctg atctgcgccg ccgtcaaagc cgggcgcatc
     1141 gaagacctca aacgtctcac cccgcgcggc gaggccgaag cgccgattgc gcccacaccc
     1201 atcgcgaaaa ttaaacccgt cgcacgcgcc gtcccgccgc ccgccgttgt cgagacagag
     1261 actcccaaga tcgtcgagac gaaagcgccg ccggtggaaa cgaaaccggc aaaggttgtc
     1321 gagacgatca cgcagagggt tagcgagagc gcgacgagcg gcgacatcct tgctctggac
     1381 gatgaaccgt cggagatcgt gccggtgatc agtgagccgg tcagaagtga gccgttgaga
     1441 agcgagccgc gacgcgtcga gccgccggaa cccgtccggc ttgtcgggag tcaaaacgac
     1501 gatttcatac aggtggaagc cgaagacgcg ctccacttga acctgctcga agagagagat
     1561 tttcatgcgg gcgaagtggt gacgctccgt atccgtgtga gtcgcggcgg gggagacgtg
     1621 cgcgtggcgg cggcggacgt gcccgtgacg gtcaaaattc tcggcacgga gtttcgcccg
     1681 ctcatcttga ccacgcgctc ggaccgcgac ggcgtggcga cgattcacgc gtggcttccg
     1741 cgcttcacca aggggcgagc cgccgtgctc gtgcgagccg caattgacgg tttctccgcc
     1801 gaactgcgcc gcgtcatctt gcagccttag ccatttgcag ccttagtcag gggacgattg
     1861 tgcgcgttca ggtcaacggg gaaatccgcg agttgccgga agggaccacg ctcggcgaac
     1921 tggtgcgtga actctcgctc gcgcccgaac ggctcgccat cgaactcaac cgcgaagtgg
     1981 tgcggcgcgc ggcctggccg gagacagaga tacgcgaagg cgatgcggta gagatcgtgc
     2041 atttcgtcgg cggcggaagc ggggaagtgt aagccgggga agatgtctca gggcgcggcg
     2101 ttgaaagcgc gacggatttg agaacccttt tcccccggtt gtcgtataat ggcattggct
     2161 ggcgctcgtc gcggcgcggc gaccggaagc ggtcgcagtc gtcgcttgaa ggcgagacgc
     2221 tcaggcgtca aacgtgatcg cccttttcat catcgcattc gtcctactcg ctgcaaccgc
     2281 atacaccttt tatcgcaggc agcacgcatc acccgactat cgcgcggaac ttcattccag
     2341 acacgcatct ccgcgcagcc tgttcgatca taaagaggca gacacggaga acgcgcggct
     2401 cgatgcggcg caggcggaca gagagattga ggagcgcaat gcgtccctgc taaaacgcgc
     2461 ggcggcgggc gacatcctga cgctcgcgga agccgcgcac gcaaacgacc ctgcgcttta
     2521 cgagcgcgtc ttacacaacc tcttggaaca ggccggggcg acggaagccg gtttgcgctc
     2581 gctcgcttcg ttcatcgcca atgattccgg cttgcgcggc agccccgacc tcgcggcagc
     2641 ctacacaaaa ctctggcagc aacaacccga ccgccagaca actgctcaac tgctccatct
     2701 cgccgcgctc tccgacgacg cgagcacgtt tgagcgcgca atcgttgcgg cgtgcgatct
     2761 tttacgcagc gggcgtttgg gtaaggtcag gccggaagag ttgtcggctt tgatcgagag
     2821 cgagtactgg gtcttgagtt ccgaagcgcg gcgcacgggc gcgggcttcg tgttgaaaca
     2881 gaggatgacg agcctgcggg aggaactgct ggcgggaaac cggaaaacgg agtcgccgaa
     2941 ggtagatagc tagaccgaaa tcgaaagact gcgcccggtg gcgtcgtggg cgcgaatgga
     3001 agcgcgtacg cctgattcaa attttcaccg ttgcatcgca acaaaggaag cgacatggac
     3061 atcaacgttc tcttttcgaa tatgccatac ctcattgtct tggggatagt ggttctcctc
     3121 ctgctcatcc tcttgagcaa gatgttcgtt aacgtaggcg ggcgcgagat cgccatcaag
     3181 gagcgtcgct atctcggcgc gaagatgccg cccggacgcg tcgttgccac cgagggcgaa
     3241 gtcggcattc aggccgatgt gctcaagccc ggcctgcacc tcatcaagtg gccgttcgag
     3301 agcgtggtgc gcaaagtgcc gctcatcgag gtgggcgcgg aggaactcgg catcatcgag
     3361 gcggtggacg gcgaaccgat gccgcccgga cgcatcttcg cgcccgaccg cgccgaaaac
     3421 gcgcacaaca atttccaaga cccgattgcc ttcatcaagc agggcggcgt caaaggcatc
     3481 caactgcgca cgctcccgcc gggcttgtgg ccgattcacc cttacctgtt ccgcgtctcc
     3541 atcgcgaagg cgaccgtcat cccgccgggc aaggtcggcg tcgtgacggc agccgacggc
     3601 gaccaactcg acgcggggcg tctgcacggc aaagccactc ccggtcatcg caactatcag
     3661 gacgcggagc agttcatcgc gagcggtggg cagaaaggtc ctcaggtcga gatactgacg
     3721 ccgggcacct accgcatcct cacgcagtcg atctccatcg aagacggctc ggagacaaaa
     3781 cacggcctct tcttcgtgcg ccactacgac gcgacgatca tcaacgagaa tcagatcggc
     3841 ctcgtcgaag cactcgacgg cgcgccgctc gacccgcgtg actacgtggc gacgcccgtg
     3901 caaggccacg ataattttca ggactgcaac gagttcatca cgggcggcgg acagcgcggc
     3961 ccgcagaagg acatcctgct ccccggcact tactacatca acccgctcgt cttcaaagtg
     4021 attcccgaat cggcgaagga agtgaagccg ggcgaggtgg ccgtgatcgt ctcgaacatc
     4081 ggcaaagacc cgggcgagga agtgcgtaag gtgatggcgg cgaaagttcg cgagcggatg
     4141 gagcgcgagg agcatgagca cgtcgaacag gctgcggcgc ggctcgacaa actcggcgac
     4201 gaaaaccgca atctggccga gatacaggaa gacctgctct ccggcgaccc ggcggatcgc
     4261 aggctcgacg agggcgcgca cgaagcctac gtcgtgcccg aaggctatcg cggcatacag
     4321 gagacggtcg ttggtcccgg tcgttactac gtcaacacgc tggcgatcac gccgatcgtg
     4381 atcccgacga cgaaccagac ggtcgaatgg acggcgggcg aagttaatca atcgttcaac
     4441 ccgttcgaag tcatttcgaa agacggcttc acgatgcaac ttgaagtgcg cgtcgtgttc
     4501 cgcgtcaaac cggaagacgc gcccttcatg gtcgccaaga tcggctcgat tgataaactg
     4561 atccagaacg tgatgcaccc gctcatcgac tcgatcttcc gcaatcaggc gtcagagtcg
     4621 tcggccatgg cctacttgca gaaccgccac gaggaacagg aacgcgccga agccagagtg
     4681 cgcgcgcacc tcttgaagta tcacgttgat gtcgtcaacg tgctcatctg ccacatccac
     4741 ctgcccgccg agttgatgaa gacgcagacc gagaagattc tcgccgagca gcggcagaac
     4801 atgtacgacg cgcagcgcga ggccgaagag aagcgcatcc aactcgcacg catcagcgcg
     4861 caggccgaca accagaaaga cctcatggcg gcgaccgtcg gcgtggacat cgcgggcaaa
     4921 cgctccgagc aacgcaaggc cgaagccgac ggcgaggcgc actacattct cgccacgggc
     4981 aaggccgaag cagagaaggt gcgcctgatg ggcgaggcgc agggcgtggc ttatcacgaa
     5041 caggtcaacg cgctcggcgc acagggcgtc gcgctcgtcg agacgcttcg tgtgatcggc
     5101 gagaagggcg tccgcatcac gcccgacgtg atggcctccg gcggcaacgg cgacggcgcg
     5161 ggcaacctcg gcacgctcct cctcctcaat ctcttccgcg accgtctacc gggaggcgcg
     5221 ggcgagaacg gggcgaaact ggcgagcggc gacggcgcgg gaaacggggc gaagttgaaa
     5281 gcctgaaacc tgcataactc gtaggctcaa gggagcggcg cgcaacctgg ctcgccgctc
     5341 cctttgccgt tcatggttga ttagttgcta taatcccgaa tcgcaccgga cggaagattt
     5401 caattttata tgtcaactac cttaatcaca caaacagaga ctttcgagat cggagggcgc
     5461 gcttttcact cgcgcctgat catcggcacg ggcaagtatc gcagcttcga agagatgaag
     5521 gcggcgcatc gcgcatcggg cgcggagatg gtgacggtgg ccgtgcgccg cgtgcccctc
     5581 gaccgcagca gcgaatcctt cctcgatcat ctagaccccg cgctcgccat cctgcccaac
     5641 accgccggat gctatacggc ggaagaggcc atccgcaccg cacgcctcgc acgcgaagcg
     5701 ctcaacacgg atttgatcaa actcgaagtt atcggcgacc agacgacgct ctttcccgat
     5761 aacgaacaga ctttagaggc cgcacgcgtt ctagttaaag agggcttcac ggtgctcccg
     5821 tatttcagcg acgatctgat catggcgaaa aagttgctgg acgcgggttg cgccgccgtg
     5881 atgccgctcg ccgcgcccat cggctccggc ctcggcgtgc aaaatcccgc gaacctgcta
     5941 atcatgcgcg agcagttgcc ggaggccacg atcattgtgg atgcgggcgt gggcacggcg
     6001 agcgatgcgg cgattgcgat ggaactcggc gcggacgccg tgctgatgaa cacggcgatt
     6061 gccgaagcgc gcgacgccgc gcagatggcc gacgccatgc gcctcgccat ccgcgccgga
     6121 cgcctcgcct acctcgccgg acggatgccg aaacgtctct acgccagcgc gtccagcccg
     6181 ttcgcgggcg tggtcaggta aacatttgag cttatgagta gcgacgctcc caaacccacg
     6241 tttaaatcgg ttgaagttaa catcttcaat caggtttata gcctgcgcac gaatcgcgag
     6301 gtcgagcaca tcaggcgaat cgccggactc gtggacgagc gcatgcgtct catctcctct
     6361 catgcgacgg cgcacgacct cgccaagatc gcgatcttcg ccgccctcaa catcgccgac
     6421 gaactgagcg acacgcgcga acactacgag cgggaatttc aaaaactact cacgcgcgcg
     6481 aacgaaagcg aaggggagcc ggaaccggca gtcgagccgg aacgcttcga agaaaccccg
     6541 ttagaggatg tgcagagatc ggaagagcgc ggctcgtggt ttgaagatat tttcgacgcg
     6601 ccggaatccg acaacgccaa aggccgtagc gagcgtctga gcagcaaaat ctccgccaaa
     6661 ctccagagcc tccgccagtc caagacggaa acctccttct ccatcgaaga aggcgattag
     6721 agcagtgacg agtgacaagt gacgagagaa ggcgttaagg gttacgtact tgtgagcctt
     6781 ctgtttttat ctcgtcactc gtcacttgtc acttattact gcgcttaagg attgggggct
     6841 ttcgggctga cgcttgaggt cgtgccgttg cgacgaaaat cggtcgcgtt attgtttgtg
     6901 tccgaggcgt tcgggctgcg gatgagatcg ttcgttgcgc cgccggtgaa agtgaagatc
     6961 gctccttcgc cgaacgcgtt gaagggggcg gacgggggcg tcgcgcctcc ctgataggcc
     7021 agcccgtcca gacgcacccc gcccatctcg atcttgactg cgcccgtcgt gttgttcaga
     7081 tcaaagccgc tcgcgctcgc atcgaagtca gccgcgacgc cgaaagtctc cgcagcgttg
     7141 gcgatgagaa aatagccgcg cggagcgagc aggaatctac cgcccgaagc atcttcgagc
     7201 gtcagggaga agggcgtgtt gccggagccg gacgggcgga acgtgacgac gaggccggtg
     7261 atgtcgagcg tgcggtcggt ggtgttgtaa agctctataa aatctcttcg cacctgcgtc
     7321 gtcgaagcgg cgaaggcgac gagggcttca ttgatgacga cctgaccagc cgcaggaact
     7381 tgcggcggcg aattcaccgt gaccgtcgcc gtgttacttg agacggggcg cgattcatcg
     7441 cgggctgtag ccgtgatgcg cgccgtgccg gttgcgcgcg ccactattgt cgcgctggca
     7501 tcgctgctgc cgggcgtgtg tctgacggag tcaatcgtcg ccacctgagt ctcgtccgac
     7561 gcgaacgtga tcgaggagac ggggaatggt tggtcgtact gatcaaatgc ctgcgccgtg
     7621 aactgttggc tgtcgccgac gttgagcgtc gcggctgcgg gagacaggcg gaggcgtgtg
     7681 agaaccggcg gcggaggctt gatcgtcagc gacgccgtgg cctctctgcc gcctgcgcgt
     7741 gcgcgaaccg acacgttccc ggcagagagg ccacgcgccg tcgtctgtct ccccgtctgc
     7801 ggcgcgacgg aggctttgcc agcgtcgctt gtgtcccaga tgaacgagac gtgagccact
     7861 tcgagttcga cgccgtcgag cactgtgtat gcacgcgcgc tgaaatcctg tgacgcgccg
     7921 tgctcaatcg tggaggctgg cggaaggatg gagatgcgcg agatgggcgt tgagtcgaag
     7981 ggcgtgccgt cggcgcgtgt gccgggcgag taagcgcgcg tcgcggcacc gggcgcgagc
     8041 gtgtgcgggt tgaagtcgcc gcccgtcgaa ccgggtgcgg cgtcgggcga gcgcgtcaaa
     8101 gattgatctg cgggcgcggg cggcgagttc ggcgcggctg tgccgtagct ttgcgaggtg
     8161 atcgtgatct cgacggagtt gagcgaaagt ttgagagaga cggtgtcgcc cgcatcgttc
     8221 aggccgagcg aggctgccgt gaagaggttt gcgccgccga aggccggatc attgtgcggg
     8281 ggcgcaccgc ctccgaagag aacgagcgcg ccgcccgccg cgagaatcgt gtgcgcggga
     8341 aaggtgtagc gacgcgcggt tgcatccgcg acggagaggc cggagatgtc caaagcctcg
     8401 cgcgaataat tcaacaactc gacgaactcg tcatcgacgg aactccgcac gccgtcgcgg
     8461 ttggcgtcgc cttcgacggc ggcggtggcc ggatcgtcgg gcgacacgtc ggcgtaaatt
     8521 tcattgatga cgagcgggac gcgcacttcg agtgacgctt gatccgagac gacgcctccc
     8581 gcgccgtccg gggtagaagc cgtgatgttg acgaggccga cgcctgtgcc gcgcgcctgt
     8641 gcggcggcgt caatcgtggc gatgaaagcg tcgctcgaag accacgtaaa agttgtccgg
     8701 tcgagcgggc gaccatcttt gtcgaaggct cgggcggtga attgaagcgc gccgccccga
     8761 ttgatagagg ccgcgtgcgg ggtgatctcc acgcgcgtca ctttgggttg aggggcgagc
     8821 acgcggagcg tcgcgggcgt ggattggacg ccgcgcgcag tggcctcgat gaacgcctcg
     8881 cccgtggcgt gcgactgcgc cagcccgtcg ccgtcaatcg tcgccactgc cgggtcgctc
     8941 gattgccagc ggaagatgac gcctcgcagt tcgttgccgt tcaagtcaaa ggctcgggct
     9001 gtgaactgtt gctgcgctcc gcgttcgatg gtcgcgtgtg cgggcgcgac ttcgatgcgg
     9061 gcgacgctga ctgtgatgtc aaacggcgtg ccgtcaacgc gcgtgcccgg cgaaaaggcg
     9121 cgcccgtcgc tgccgtgcgc ggtctggtgc agcgcgaagt cgcctaaggc atccggcgcg
     9181 cgcgtcagcg attgattgcg gtcggcgtca aggccgtttg tgtcgccata agtgaacagg
     9241 tcaatggttg cgcccgccgg gtcgagcagt ttgacggtgc tgccgccatt ggtgagagag
     9301 aggccgcccg tcgaagcagg caggacgagc gcgccgccga atgccggatg cgcggggttg
     9361 aacgtagagc cttgcgcgcc gccgaacacg acgatggagg tgccgggcgc gaggatggta
     9421 tccgccgcga aggtgtggcg cacgatgtcc gtgttgttgc tgttgcggac tgtgagttgg
     9481 tagccgccga ggtcaaggtc gcgcgtcgag gcattgacga gttcgacaaa ttcgtcctgc
     9541 gcggaactgc gcgtgccgtc tcgattggcg tcgcccgccg cgccttcggg cgggtcggcc
     9601 agaatctcgt tgatgacgag ggagagcggg aagaccgtga gttgagccga cgcagagact
     9661 tcgcccgaca cggccttgat cgtcgccgtt ccggcgcgga cgccgcgtgc gcttccatcc
     9721 gcatcaatgg tcgcgattgt cgcatcttcg gaagtgaaag cgacgggcgc gtttgtgatc
     9781 tcgacgccat ccgcatcgcg cacggtggcg acgaaacgca cagacctgcc gacgcctatc
     9841 gaagcgttcg acggttcgag cgtgatgctc gcggcgcgac gcgtgccttt gaaattgagc
     9901 gtcgtcgggt tgctgtcgcg cccgtcggcg cgcaggagca gcgggacggt tccggctccg
     9961 gcgagcgaac gcgagagccg gatatgaact tcgtccagac ccggcagatc gggcgagggc
    10021 agtatctttt ccacgtccaa ctcacgcccg ccgattgtga ccgtgacgtg ctttgcgtgg
    10081 cgaatgccgg tggtgaagat cgtgagccgt cgcggttggt tggcggcgtc aacggggtcg
    10141 aaagggctgc gcaaaaatgt ctccgcgtcg agcgcgatgg ccgcgccgct cccgtcgccg
    10201 cgctccgtga agacgccggg cgcgacgtgc agcgaggaga cggaagcgcg cgattcgtag
    10261 ccgtcgtgat tgcgcacgac gacgtgcgcc gcgcctgtct cagtctcagc cgggaccaga
    10321 aagttgatct gtgtgggcga ggcgtaaaag agttgcgcgc tgcgcccgtt gacgtagagc
    10381 gatgcgcctc cgagcgtccg tgggaatgtg ccgccgggca gacgcttcgc ctgtatcgca
    10441 tcgagggcaa ggttgacgcc ccgcgccgac gcgatctgtc cgggtgcgat ggctcgcgtg
    10501 gccgcaggtt ctctgtcgag agatgcgccg ttgaggatct gtaagccggt ggagaagggc
    10561 gggcgcgcat cgagccgtgc gagatagatt tccgtgtggt tgacaaaact gctgtcggag
    10621 attgttccgc tgagcaggcg ggggaagttg aaggcgacgc gcgagccgtc ttcgttgagc
    10681 gaagagacga cttcggcggt cgcggcggca ggcgcgtcgg tgacgcggga gactctgttc
    10741 gtcggcagat cgtagacgta aagctcgacg ctcgcatcgg aattgccgcc gttgacattg
    10801 cgccgcgtag caaaggcgat gcgtgagccg tcgccgctga ttgtgggatg gagcggcacg
    10861 tctgcggcgc gcgagccgag cgaggtgagt tgacgcaatt gatcgttgcg cccgtcgtag
    10921 agaaaaatct gcgttgtgcg cgccgccgtc tgcgccgcat agacgacgcg tgcgccgtcg
    10981 tcgctcgtcg cgcggccata ggtcagagcg aggccgtcta catctttggc gatcacgcgt
    11041 gtcgagcctt gagcgcgctc gtagatgagc aagtcgcgct gcggagaaga ctcgacgccg
    11101 cccgtcgcgc cgttgtctcg cacgaaggcg acgcgcgctc cgttgccgct caacttggcg
    11161 tcggttgcgc cgttgatgcc ggacgtgtgc gtgatctgct cggtggcgcg ggtggcggaa
    11221 tcgtagacga atatttcgaa attcgcgtcg gggttcgcgc ctgtcaggtc gcggttggaa
    11281 gcgaacgcga gcaaggtgcc gtcgttggag atggagggct ggaaatttcc ctcgacgatt
    11341 cgttgtgcgg cgtcgcgggg cgaggtgtcc gtgagttgtt gtagccgctc gccgtcgtag
    11401 agaaatattt ccgagttgcc gtctgaattt tcgccggtga gattttcacg gctggcaaag
    11461 gcgaggcgcg agccgtcgtg cgagatggcg ggcgcgggtg cgcgtgtggc tgcgatgcgt
    11521 gtgaaggtcg cgccgtctgc gtagatgtcc gcgcggagag tgtggaagcc tgtgccgctg
    11581 ccgcccgcgc cgccgatctc ttctgtcgat tcaaaagcga tgtgctgccc gtcgccgctg
    11641 atgctggggt tgagattgag tgcccgctcg gatgtcagcg tgatgcgacg cagcggcgcg
    11701 tcgttttgcg agcgcacgcc cgcgacgacg acacagaggc cgagcgcgag ggcgaggcac
    11761 agggaagagg cgaacaacct tgtgtgagac ttcaaggcga tgctcctgtc gccgtgtcgc
    11821 cggtgaatga cacgggggtt cgagttgaga gaagttcggc gaaaaggggt gggcttaagt
    11881 gtcgagcgcg cgagcgttca gagttttaat ggacgactcg gatgtcgaac gcgtgtgtcg
    11941 atttgaagtg cgacgagagg cgaaccgggc gaatgcgtga ggcgcgcacc gcttcaactt
    12001 gacgcgcggc attctacagc tttcaaagtg aaaagcaagc gtggccggga cggccttttc
    12061 ccggcgcacg ctcgactgcg gttaacctgc ggctcgcgtc gtctccctcg cggctccgaa
    12121 ttccggcaga gcctcgaaga cttcgagcgc gtagcggacg ttgttgaaag gggcggagac
    12181 ttgcacgccc tgaataagat cgcgcacttc gagcagagac tcgcgcgcga tctgcaagcc
    12241 ttcatcgcgc gccgcctctt tgccggtttc cgaggcgcgg cgcatgcgtt ccatgatcga
    12301 aggcgtgacg cgcacgcccg gcacttcgtt gtgtagaaac tccgcgttgc ggtaggagac
    12361 gagcggccag atgcccgcga tgacgggaat ccgcacgtgc tcgatcaact tgagaaagtc
    12421 gcgcagttgt cccacgtcga agacgggctg cgtgatcgcg tactccgcac cggcctccac
    12481 cttgtactcg aaacgcttga tctcttcttc cagattgatc gcgccgggat tgacgccgac
    12541 gccgatgcag aaagaggtcg ggcagccgat ggggttgttg ccgagatcga ggccgtgatt
    12601 gagcttgttg accatgttgg tcaggccgat agagtcgatg tcgaagacgg cggtggcgtc
    12661 cgggtagggt cccatcttcg gcgggtcgcc ggtgatgagc agaaggttgc gcagtccgag
    12721 cgcggctgcg ccgagcaggt cggacatcat gccgagcagg ttgcggtcgc ggcaacaata
    12781 atggagcacg gcttcgagtc cgatctcgcg ttcgaccaga agagccgtgg cctgtgcgct
    12841 catgcgcgtc tgcgcgcgtg gcccgtcggg gatgttgacg gcgttgaccc cggcggtctt
    12901 gagcaggcga atctgttcga gcgtctttgc cgcgtcgcag cctttcggcg gcagcacttc
    12961 gaccgtcgtg acaaactcgc cgttcacgag acgacgcgcc cagtttgaac gctcctccat
    13021 cggtacgacc tgaatgtcgg cgggctgcaa ctcttcgatg cgcgcgggct gcgaaggctt
    13081 gagcgcggcg cgtgctttgc gcggcgaggc ggcgcgcacg gcgtcggaga tgagcttgat
    13141 gtgcgagggc gtcgtgccgc agcaaccacc gatcactttc acgcccgcct gaatcagacg
    13201 cttcgcatac ttcgccatgt attccggcga gcacatgtag aattgccgcc cctgcacgtc
    13261 gcgcggcagc ccggcgttcg gttgtgcggc cagccgcttg ttcgtcaccg tgcgcatccg
    13321 ctcaatggcc gtgagcacgt ctgcggggcc gacgccgcag ttgaggccga tcacgtctgc
    13381 gccccattcg tcgagccgcg tggtgaagac ttcgggcgtc gtgccgtagg aagtgttgcc
    13441 gtccgagagg atcgtcatct gcgcgacgat gggcaggtcg cacagttcgc gcacggcgcg
    13501 cagagcctgc cgtatctctg agatgtcaga gaaagtttcg aggacgaaga gatcgactcc
    13561 gccttcgagc agcgcgccga cctgttcctt aaacatctcc ttcgcttcgt cgaaagaggt
    13621 ggggccgtaa ggctcgatgc ggaggccgag gggtccgatt gcgcccgcga cgtagcagcg
    13681 ttcgcaggcg gcttcgcgcg ccagttgcgc cgccgtgagg ttgatcttgc gcaggctccc
    13741 ttccagcccg tactgctgca agttgtgcat cgtcgcgccg aaagtgttcg tctcgatcac
    13801 gtctgcgccc gcgcgcacgt actcctgatg cacctcgcgc acgaggtctg gattcgtcaa
    13861 gttcagttcg tcatacgaac gattgatata gacgcccttt gcgtagagca tcgtgcccat
    13921 cgcgccgtca aacacagtga tctgatcgct cgccagtaag tctcgaaagt tttccatgat
    13981 agaacagtga caagtgacag gtgacgagtt ggaaagacaa gttgttttaa ctcgtcactc
    14041 gtcacttaaa aaaaactccc gcgccaaaca acgcgggact tcaaaaaaag cgtcttgaag
    14101 tgtgtgatgc tcttgccgag aagagccgtg ctgtttagct gtttatttta cgtggccgca
    14161 agtcgcctta actcaccacg tctttgctca acgcgcttat actagcgtca gcccgcgact
    14221 gtcaagcgcg ggttggaggc tcgctttacg gttgatgcgt gtgagctttc gagagagggg
    14281 gcgaggccgg acgcgcgaaa agctcaagcg gcagggaatg acgtttctcc tgccgcttga
    14341 gctttctgtc gcctcgatct tccggttcgc gggggaaaca gtttaaggat cgatatctcc
    14401 gccttcggcg tcgggcagag ggaatatcgt ctcggtcttc gtcacgttgt tctctttgtg
    14461 gagttcgtct gtgtagctcg aaatatagga caggctgctg aagaccgtgc cttcccgaac
    14521 gtcgcggttc acaaggtagt agacggcgaa ctgcgctgtc tcgtcaacct gcaagccctt
    14581 cgtgttacaa acgattcttc cggtttcgcc gagggctggg gtcgagcact cgaagaaagg
    14641 accggaaact tgtttcagcg cgtagaaggt catgccggac ggaacttcgt cgatgatctg
    14701 gacgccctcg gcgaaatcgg gtcccgggtt gtggaacgac aggttgtacc tgaccctttg
    14761 ccccggatac acgtattcgg gcgcggccat ggagatggag aggtctatga aaggcaggcg
    14821 cgggttgcgc acgtagaact taccgttgac cctacggcca gagtggacgt tgatggtcgc
    14881 cgcatgccac gttccgactt gcgctaacgc gccggaggtc ggaatctcga aatattcggt
    14941 ctgcgggtcg ctgtagaaga gagcctgatt ggcgaccgtg tgattgggcg cgatccagga
    15001 gaggcgtcgg tagttgaagg cgtcgagggg aagagggaag ttgcccgccg taatgcagac
    15061 agtgtcgccc agatagaaga cggtctgagg cgtccggcag tctttggcgt agactgtgac
    15121 gtactctgct gcgccctctt cataggcggc agcgacggct ggattagcgg tgcgcgtagg
    15181 gtcggaagcc gtggaggcgg aaaatgctcc ggccagcgtg gcgataaaca tgaagagtga
    15241 aacggcgaat gtg
//
LOCUS       DASWNS010000234        10415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_217509, whole genome shotgun sequence.
ACCESSION   DASWNS010000234 DASWNS010000000
VERSION     DASWNS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217509"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2512)
                     /locus_tag="VGB76_08095"
     CDS             complement(<1..2512)
                     /locus_tag="VGB76_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEY0098901.1"
                     /translation="MRQRHTLSSCACIMLLFCFGAARFVEAQAPEQSPPRLSVQKGHE
                     ESVYRMAFSADGRTLASSSLDKSVRLWDVEKGWELGVLDAEDEMLSDLVFSGDGRRLV
                     GYKNSVIIWDVATGRKLHSYAPGLLMGLEQYLVSFDGRRLLVVRETNAVEVLEAETGR
                     VLKTLLRGEQRRLDEVTRRVAAAPDWKTFFVYGEGGLVRALDASSGATRFSFKASPRR
                     LSLSLDGALLAFADEATRALVVSDAATGRELWRKPDANSPWLTFDASGKQLIAFHETG
                     VAAFDARTGQEAGKWPGVRSNPADNKFERELSFLPLPDGSGYAFNPPNPRNIEIDILN
                     RDSRVLRTLAGRASTIADVAFSPDGRTLVLAPNDHVSGDSREDDAVFLLNYADGTQGR
                     ALIQPKGGVMHLAYSPDGKRLATAGLTSLTLWDLEKGGVLSELESSVSYGNLAFSPDG
                     KLLASFNYKNLTLWDTSSGQVVRQLSENKETVHFVAFSPDGRLLVAVNNVRKEVVLWD
                     VATGQPARTIPQVSGTLAFSPGGKVFAANDPEGHVIFVDPTTGATVSRVAAAPARASA
                     RQMLVGHFRLTYSPDGKRLAAVAIVLNPQGKVRLQMQGSPQRADVFIRLWDTATGAEL
                     RTLDGHSEFIMSLAFSGDGRYLLSGGQDRLAKLWEVESGRELASLTPVGRDDWIVMTP
                     DGLFDGSPAAWSAILWRFSDTLRDAAPVEAFFNEYYYPNLLTELLRGKHPSAPGGGLA
                     ARDRRSARLDLNLSGASADTDVLAAREVKVRLDVSDAPAGAKDVRLFRNGSLVRVWRG
                     DVLKGAPRVTLEATLPIVAGENHLTAYAFNRDNVKSPDA"
     gene            complement(2714..3784)
                     /locus_tag="VGB76_08100"
     CDS             complement(2714..3784)
                     /locus_tag="VGB76_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0098902.1"
                     /translation="MPAMISYIFPLFVCVALVWTPFVQQPGAAKKTPEAEIIEAIGDR
                     EAEKVKELLAKTPALAKYRSPGESVTLLHSAAQTSDNEEVFKVLLAAGAEVNARDKNG
                     ETPLHLSARWGYTPHGVLLLDAGADPNAQGQDGETPLFGTMETDYHLAEELLKRKADP
                     NLKNSDGVTALYRAAALGDLKTLELLAAFKADPNIKANDGQTALCGVAREGQEDAVEM
                     LLALKADPNIADNEGKTPLHYAAEGGRKLILSMLIAAGAAVNVKESGGATPLHLAAGK
                     GHVRLVALLIAAKADVNSRDGYERTPLHRAAREGYASVVAALLSAKADAALKDTNEQT
                     PLELAEEFKRHAAAAALKSPGK"
     gene            complement(3923..7306)
                     /locus_tag="VGB76_08105"
     CDS             complement(3923..7306)
                     /locus_tag="VGB76_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0098903.1"
                     /translation="MQSGRSWRVYAMLLALTLGGAWWVAVGRVSAQTDDLEEAKRLNR
                     EVVRLHGAGRYDEAVPLAERALAIREKALGAEHPAVAQSLNSLAILYEAKGDYARAEP
                     LYERTLALREKLLGAEHADVATSLNNLAGLNYLKGDYARAEPLYQRALAIREKLLGVE
                     HPGIAAPLNNLALLYHVKGDYKRAEPLYQRALSIREKNLGAEHPDLALSISNLAGLYD
                     STGDYVRAEPLYKRALAIFEKALGAEHPEVATSLDNIAGIYRTKGDYASAEPLYQRAL
                     AIYEKVLGTEHPSVADSLNNIAALHYERGDYGRAELLHQRALAIREKTLGATHPAVAS
                     SLGNLALLYEVKGDYGRAEPLYLRAIAIREKALGGEHPEVALTLNNLALLYQARGDYA
                     RAEPLYRRALAIREKVLGTQHPDVAISFNSLAVMYHVKGDYASAEPLYQRALAISEKA
                     LGAEHPEHAQALNNLAGMYHDSGDYVRSEPLHQRTLAIREKVFGAAHPAVASSLNNLS
                     DLYEAKGDYERAIAGRSRSADIREQNITLILTTGSEKQKQLYLDTLSGETDTVVSLNI
                     KGAPKNDAATRLALNTILRRKGRALDAMTDQIAALRRRAGTEDQKLLDQLAAARSQLA
                     NLQLGGASSNLTPAARQAQLVALAEEIEKLEANIGRRSAEFRVQAQPVTLSNVQAALP
                     AGAALIEIFSYRPYNTKAQPGNKFGAARYVAYVSRREGEPQFVELGEAAAIEANVEKL
                     RRTLRDSRQAARGDVSAVKEAARVVDEQVMRPVRGLLGATRRIFLSPDGALNLIPFAA
                     LVDERGKYLVEDYTLTYLTSGRDLLRLQASDESKSPPLVLANPLYNDSGGNTGSQVET
                     LSAVAAKRDVDFNAIDFSKVSYVPLPGTADEAKALSALMPGARLLSAAEATEAALKQV
                     HAPRILHVATHGFFLTDKPRQADSRGVGLSSTTPAVASRGENLLLRSGLILAGVNQRR
                     SGTDEDGVLTAAEAAALDLWGTKLVVLSACETGLGDVKNGDGVYGLRRALVLAGSESQ
                     VMSLWQVSDAATRDLMKAYYARLQSGEGRTEALRQVQLEMLKGTAAVQGGQQERTLLG
                     SSGAKAQASNYHHPFYWASFIQSGAWSGIESGTTAGGCQ"
     gene            complement(8007..8882)
                     /locus_tag="VGB76_08110"
     CDS             complement(8007..8882)
                     /locus_tag="VGB76_08110"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0098904.1"
                     /translation="MTEDPKEQGAKPPFEEQPQDVPGIESEMTPKPDYGADSYRGHGR
                     LANKAALITGGDSGIGRAVAVAFAREGADVLISYLAEEESDARETARVVEEAGRRCVA
                     VAGDIQDESHCQGLVERAVQEFGRLDVLVNNAAFQMTRDGIGEITSEEFDRTLRTNVY
                     AMFYLCKAALPHLKEGGSIINTASIQAYQPSPELLAYAATKGAIVTFSKALGEEAMKQ
                     GVRVNVVAPGPVWTPLIPSTMPDEKVESFGQNTPAGRPAQPAELAPVYVFLASQESSY
                     VCGEVIGVTGGKPLP"
     gene            complement(8934..9188)
                     /locus_tag="VGB76_08115"
     CDS             complement(8934..9188)
                     /locus_tag="VGB76_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HEY0098905.1"
                     /translation="MDGITWLGLAAGGLTTASFVPQVTKIWKTKSAEDVSLTMFLAFC
                     VGVSLWLVYGFIKGEVAIIVTNAVTLALGAAIVWMKLKFK"
     gene            complement(9486..>10415)
                     /locus_tag="VGB76_08120"
     CDS             complement(9486..>10415)
                     /locus_tag="VGB76_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0098906.1"
                     /translation="VAGESPASGAQDVFTDNSSSLYVLDQPGSLYLNSVETGYFETLE
                     IPLLRGRDFTERDDQSSPDVIIVNETLARRLSPDGNALGKQLVERDPFNKKHRFMEVV
                     GVARDSKYLWPTERPRYFAYRPLKQDTPGQVSLIVRSDGDRQAITNSIHSVAGSLDAN
                     VPSNEVLTLEEIIDGHVGSSKVLIWLAGLLGFLATGLASIGLYGVTAYAVSGRTKEIG
                     IRMALGAKGSQVLRLIIFEGLTLILTGILIGLFLSVVGTRAMASLLYGVSPTDPLTFT
                     GVAALLTLVALIACYVPARRATKVDPMVALRYE"
BASE COUNT         1809 a         3290 c         3198 g         2118 t
ORIGIN      
        1 gcgcgtcggg acttttgacg ttgtcgcggt tgaaagcgta ggccgtcaaa tggttctcgc
       61 ccgcgacgat gggcaaggtg gcttcgagcg tgacgcgcgg tgcgcccttg agcacgtcgc
      121 cgcgccagac gcgcacgagc gaaccgttgc gaaacaaacg cacgtccttc gcacccgcag
      181 gcgcgtccga cacgtcgagc ctgactttca cctcgcgcgc cgcgagcaca tccgtgtcgg
      241 cagacgcgcc ggacaaattt aagtcgaggc gtgccgagcg gcggtcgcgt gcggcgagtc
      301 cgccgcccgg cgcggaggga tgtttgccgc gcagcaactc ggtcaacaga ttcggataat
      361 agtattcgtt gaaaaacgct tcgaccgggg cggcgtcgcg cagtgtgtcg gagaatcgcc
      421 agaggatggc cgaccacgcg gcgggcgaac cgtcgaagag tccatcgggc gtcatcacga
      481 tccagtcgtc gcgaccgacg ggcgtgagcg atgcgagttc cctgccgctc tccacctccc
      541 acaacttcgc gaggcgatcc tgtccgccgc tgagcaagta tctgccgtcg ccgctgaacg
      601 cgagcgacat gatgaactcg ctgtggccgt cgagcgtgcg cagttcggct ccggtggctg
      661 tgtcccagag gcgtatgaac acgtctgcgc gctgcggtga accctgcatt tgcagacgca
      721 ccttgccctg cggatttaag acgatggcaa ccgccgcgag cctcttgccg tcggggctgt
      781 aagttagacg gaagtgcccg acaagcatct gcctcgcgct cgcccgcgcg ggggcggcag
      841 ccacgcgact cacggttgcc cccgtcgtag ggtcaacgaa gatgacgtgt ccttcggggt
      901 cgttcgcggc aaacactttt ccgccggggc tgaaagcgag cgtgccggag acttgcggga
      961 tggtgcgcgc gggctgtccc gtcgccacgt cccacaacac gacctccttc ctgacattat
     1021 tgacggcgac cagcagtctg ccgtcaggac tgaaggcgac gaagtggact gtctctttgt
     1081 tctccgaaag ttgacgcacg acctggccgc tcgacgtgtc ccacagcgtc aaatttttgt
     1141 agttgaagct ggcaagtaat ttgccgtcgg ggctgaacgc gaggttgccg taagagacgc
     1201 tgctttccag ttcgctcaag acgccgccct tctccaaatc ccagagcgtg agcgaggtca
     1261 aacctgccgt cgccagacgc ttgccgtcgg ggctgtaggc caaatgcatg acgccgcctt
     1321 tgggttggat gagcgcgcgc ccctgcgtgc cgtcggcgta gttcaagagg aagacggcat
     1381 catcctcacg gctgtcgccg ctgacgtgat cgttcggggc gagcacgagc gtcctgccgt
     1441 cagggctgaa agccacgtcg gcaatggtcg aagcgcgccc ggcgagagtg cgcagcacac
     1501 ggctgtcgcg gttgagaatg tctatctcga tgttgcgcgg attgggcggg ttgaaagcgt
     1561 agccgcttcc atcgggcagg ggcaggaagc tcaactctct ctcaaatttg ttatccgccg
     1621 gattcgacct gacgcccggc cacttccccg cttcctgtcc cgtgcgcgcg tcgaaggccg
     1681 cgaccccggt ttcgtgaaag gctatgagtt gtttcccgct cgcgtcgaac gtcagccagg
     1741 gcgagttggc gtccggcttc cgccatagct ctctgcccgt cgcggcgtcg gagacgacga
     1801 gcgcgcgtgt cgcttcgtct gcgaaagcca gtagtgcgcc gtcaaggctc aggctcaggc
     1861 ggcgtggcga cgctttgaat gagaaacgcg tcgcgccgct cgacgcatct agcgcgcgga
     1921 cgaggccgcc ctcgccgtag acgaagaacg ttttccagtc gggtgcggcg gcgacgcgcc
     1981 tcgtcacctc gtccagcctg cgttgctcgc cgcgcagcag ggttttcagg acgcgcccgg
     2041 tttcggcttc gagcacttcg acggcgttcg tctcgcgcac gacgagcaga cgccgcccgt
     2101 cgaaactgac gaggtattgc tccagcccca tcagcaggcc gggcgcataa gagtggagct
     2161 tccgcccggt cgctacgtcc cagatgatga cactgttttt gtagcccacc agacgccgac
     2221 cgtcgccgct gaaaaccaga tcactcagca tctcgtcctc tgcgtcgagc acgcccaact
     2281 cccagccctt ctccacatcc cagagccgca cggatttatc gaggctgctg ctggcgagcg
     2341 tccgcccgtc agccgagaac gccatgcggt agacgctctc ctcgtgcccc ttctgcactg
     2401 agaggcgcgg cggcgactgc tccggcgctt gagcttcgac gaatcgcgcc gcaccgaagc
     2461 agaacagcaa catgatgcag gcgcacgaag agagcgtgtg tctttgtctc attttctatt
     2521 tacgtttgaa cgggatgatt cagacttcgg ggttgaagca cgagcgcagc cacgcgctcg
     2581 aaggatgaac tgcgccttgc cctgacatca atacgggtct gagcgaaaga acttgaagcg
     2641 gtggcgcgtc tttaacgtcg ctttctttag cgtcgcttcc tttagcgtcg ctccggtgcg
     2701 ctatccgggc gcgttatttg ccagggcttt tcaacgccgc tgccgccgcg tggcgtttga
     2761 actcttccgc cagttcgagc ggcgtctgtt cgttggtgtc tttgagcgcc gcatcggctt
     2821 tcgcggacag caacgcggcg acgacgctgg catagccttc acgcgccgcg cggtgcagag
     2881 gcgtgcgctc gtaaccgtcg cgtgagttca catcggcttt agccgcgatg agcaaggcga
     2941 caaggcgcac gtgccctttc ccggcggcga ggtggagcgg cgttgcgccg ccgctctctt
     3001 tgacgttgac cgccgcgccc gccgcgatga gcattgagag gatgagcttg cgtccgccct
     3061 cggcggcgta atggagcggg gtcttgcctt cgttgtcggc gatgttcggg tcggctttca
     3121 gtgcgaggag catttcgacc gcatcctcct gcccctcccg cgcgacgccg cacagcgcgg
     3181 tctgcccgtc gttcgccttg atgttcgggt cggccttgaa tgccgcgagc agttccaacg
     3241 tcttgaggtc gccgagcgca gccgcacgat agagggctgt gacgccgtcg ctgttcttca
     3301 aattcgggtc ggccttccgt ttgagcaact cctcggccag atggtagtcg gtttccatag
     3361 tgccgaacag cggcgtctcg ccgtcctgtc cctgtgcgtt cgggtcagcc ccggcgtcga
     3421 ggagcaagac cccgtgcggc gtataacccc agcgtgcgga gagatgcaac ggcgtctccc
     3481 cgttcttatc gcgcgcgttg acttccgcgc ccgccgcgag caggactttg aagacctctt
     3541 cgttatcgga agtttgagcg gcggagtgca gcagtgtcac gctctctccg ggcgacctgt
     3601 atttcgccag agccggagtc ttcgccagca actctttgac cttctctgct tcgcggtcgc
     3661 caatcgcctc gatgatctcc gcctccggcg ttttctttgc cgcgcccggc tgctgcacga
     3721 agggcgtcca gacgagagcg acgcagacga agagtgggaa tatgtaggag atcatagcgg
     3781 gcattttttg gccttctgtt taaaggttag agcagccgcg ttgaatcgcg gcggacggca
     3841 ttgcctgatt cttttaagcc atttaataac gaccagtcaa agccgtgcgg gtgcgaccgg
     3901 aaacaagccc gaccgccgtg aattattgac acccgccagc cgtcgttccg ctttcaattc
     3961 cagaccacgc gccggactgt atgaagctcg cccagtagaa cgggtggtgg tagttcgacg
     4021 cctgcgcttt tgcgccggat gagccgagca gtgtgcgttc ctgctgcccg ccctgaactg
     4081 ccgccgtccc cttcaacatc tcaagctgca cttgccgcag agcctccgtg cgcccttcgc
     4141 cactttgcag gcgcgcgtag taagccttca tcagatcgcg cgtggccgcg tcggacactt
     4201 gccacaggct catgacctga ctttcgctgc ccgccaggac gagcgcgcgc cgcaggccgt
     4261 agacgccgtc gccgttcttc acgtcgccga gtcccgtctc acaagccgac agcacgacga
     4321 gtttcgtgcc ccatagatcg agcgcggcgg cttcggcggc ggtgagcacg ccgtcctcgt
     4381 ccgttccgct ccggcgttga ttgacccccg ccagaatcaa gcccgaacgt aacaacaaat
     4441 tctcgccccg cgaagcgacg gcgggcgtcg tgctcgacag gccgaccccg cgactatccg
     4501 cctgacgcgg tttatccgtg agaaagaaac catgcgtggc gacgtgcagg atgcgcgggg
     4561 cgtgtacctg ttttaacgcc gcttcggtgg cttcggcggc agacagcagg cgcgcgcccg
     4621 gcatcagcgc gctcaaagcc ttcgcctcgt ccgccgtgcc cggcagcggc acgtagctca
     4681 ccttactaaa atcaatcgcg ttgaaatcaa cgtcgcgttt cgcggccacc gcagagagcg
     4741 tttcaacctg actgccagtg ttgccgccgc tatcattgta gagcgggttt gccagcacca
     4801 gcggcggcga cttgctctcg tccgaagcct gcaagcgcag caggtcgcgc ccgctcgtca
     4861 ggtaagtgag cgtgtagtct tcgacgaggt atttgccgcg ctcgtccacc agcgcggcga
     4921 aagggatcag attcaaagcc ccgtcaggcg agaggaagat gcggcgggtg gcaccgagca
     4981 acccgcgcac ggggcgcatc acctgctcgt caacgacgcg cgccgcttcc ttgactgccg
     5041 agacatcccc acgcgccgcc tgcctgctgt cgcgcaacgt ccggcgcagc ttctcgacgt
     5101 tcgcctcaat cgccgctgct tcgcccaact ccacgaactg cggctcgccc tcccggcgcg
     5161 aaacgtaagc cacataacgc gccgcgccga acttgttgcc cggctgcgcc ttcgtgttgt
     5221 aaggacgata ggagaagatt tcaatcaacg ccgcaccggc gggcagcgcg gcctgcacgt
     5281 tcgacagagt gacgggctgc gcctgcacgc gaaactccgc gctgcggcga ccgatgttcg
     5341 cttccagttt ttcgatctct tcggcgaggg cgacgagttg cgcctgccgc gcggcgggcg
     5401 tgaggttgct gctggcgccg ccgagttgca ggttggcgag ttgcgagcgc gcggcggcca
     5461 gttggtcgag cagtttttgg tcttccgtcc cggcgcggcg gcgcagggct gcgatctggt
     5521 cggtcatcgc atcgagagcg cgccctttgc gccgcaggat ggtgtttaag gccagccgcg
     5581 tcgccgcgtc gttcttcggt gcgcctttga tattgagcga gacgacggta tccgtttcgc
     5641 cggagagggt gtcgagatag agttgtttct gcttctcgga gccggtggtg aggatcaacg
     5701 tgatgttctg ttcgcggatg tctgcggagc ggctccggcc tgcgatagcc cgttcataat
     5761 cgcctttggc ttcatacagg tctgacagat tgttgagcga agaggcgacc gccgggtgcg
     5821 ctgcgccgaa gactttctcc ctgatggcga gcgtgcgttg atgcagcggc tcggaacgca
     5881 cgtagtcgcc gctgtcatgg tacatgcccg ccagattgtt gagcgcctgc gcgtgctcag
     5941 gatgctccgc ccccagagcc ttctcgctga tcgccagcgc gcgctggtag agcggctcgg
     6001 cgctcgcgta gtcgcctttg acgtgataca tcacggcgag gctgttgaag gagatggcga
     6061 catcaggatg ttgcgtgccc aatactttct cacggattgc cagcgcgcga cggtagagcg
     6121 gctcggcgcg tgcgtaatct cctctggcct gatacagcaa ggccagattg ttgagcgtca
     6181 gcgcgacctc agggtgttcc ccgccgagag ccttctctct gatggctatc gcgcgcaggt
     6241 acaaaggttc ggcgcgtccg taatcgcctt tcacttcata cagcaaggcc agattgccga
     6301 gcgaagaggc gaccgccggg tgcgttgcgc ccagagtctt ctcacggatt gccagcgcgc
     6361 gctggtgcag caactctgcg cgtccgtagt cgcctcgctc gtagtgcagc gcggcgatat
     6421 tgttgagtga atcggcgact gacgggtgtt cagttcccaa gaccttctca tagatcgcca
     6481 gcgcgcgttg gtagagcggc tcggcgctcg cgtagtcgcc cttcgtgcgg tatatcccgg
     6541 ctatattgtc gagcgacgtg gcaacctccg gatgctccgc gccgagtgcc ttctcgaaga
     6601 tggcgagcgc gcgtttgtag agcggttcgg cgcgcacgta gtcgccggtg ctgtcgtaca
     6661 gtccggccag attgctgatt gacagggcga ggtcgggatg ctccgcgcct aagtttttct
     6721 cacgaatcga gagcgcgcgt tggtagagcg gctcggcgcg tttgtaatcg cctttgacgt
     6781 ggtagagcag ggcgagatta ttgagcggcg cggcgatgcc ggggtgttcc acgcctaaca
     6841 gtttctccct gattgctaag gcgcgttgat agagcggctc ggcgcgtgcg tagtcgcctt
     6901 tgagataatt gagtcccgcc agattgttga gcgacgtggc gacatctgca tgctccgctc
     6961 cgaggagttt ctctctcagg gcgagggtgc gctcgtagag cggctcggcg cgcgcgtagt
     7021 cgcctttggc ctcgtacagt atcgccagac tgttgagcga ttgcgcgacc gccgggtgtt
     7081 ccgcgcccaa cgctttctca cggattgcca gcgcgcgttc ggccagaggc acagcttcgt
     7141 cataacggcc tgcgccgtgt agtcggacca cctcccggtt gagccgcttg gcctcttcca
     7201 gatcatccgt ctgcgcggag actctgccga cggcgaccca ccatgcgccg ccgagcgtca
     7261 aggccagcag catcgcgtac acgcgccagc ttcttccgga ttgcatagtt gtttacgctt
     7321 cttccttaac gagaaatgtt ttttgagctt ccctctggat aggttcacgc cgggcgcgtg
     7381 gcaaagatta ttctgaggca cgacgaaaga gctgcaaatg agtctgacgc ctcgcgccct
     7441 ctgtgcatca tgccgggaat gagtcgtagc aataaagatg cagcgtcgat ataaaccgcc
     7501 cgcatccgta actttccttc gctacgcctg tatagttcta cctgataagt ctgcctatga
     7561 ccagcaggac tctgccgata aatctgcccg acaacaggac gaagaaggat tcctttgccg
     7621 gtaaacgact atggcaaatc tacgggtcgc actagatacc gccggaggtt gccgggaacg
     7681 ccttgagtcc ggctacttgc ttcttgcgcg cggcagtgat ggcacgatga atttgtgaaa
     7741 ggtaagcgcc atttaatgag gcggggtcgc ggacattatc ttcgagcgcg cgaccgtgcg
     7801 gcaaaaatct tttagcccca tgactaagtt aggagcagat gcggacgggc gcacggcagc
     7861 agtctgaggc taccgtgcgc ccgtctaaaa acgaaaacgc gtcatcgtct acagatgagg
     7921 ctactgtcgg gtcgtgaaag atagctgcgt ataattcccg cagcgcccga cactttgctc
     7981 gacgctcttc aaggtcgctc cgcgcgttac ggcagaggct tgccgcccgt cacgccgatg
     8041 acctcgccgc agacgtagct cgactcctgc gaggcgagaa agacatagac gggcgcgagt
     8101 tcggcgggct gcgcgggacg ccccgcaggc gtgttctgcc cgaagctttc aaccttctca
     8161 tccggcatcg tcgagggtat cagcggcgtc cagaccggcc ccggcgcgac gacgttgacg
     8221 cgcacgccct gcttcatcgc ctcttcgccc aacgccttcg agaaggtgac gatggcgccc
     8281 ttggtcgccg cgtaggccag cagttcgggc gaaggctggt aggcttgaat tgacgcggtg
     8341 ttgatgatcg agccgccttc tttgaggtgc ggcagggcag ccttgcagag gtagaacatc
     8401 gcatagacgt tggtgcgcag ggtacggtca aactcctctg acgtgatctc gccgatgccg
     8461 tcgcgcgtca tctggaaggc ggcattgttg acaagcacgt cgagccgccc gaactcctgc
     8521 acggcgcgct cgaccaaccc ctgacagtgt gactcgtcct gtatgtcgcc cgcgacggcg
     8581 acgcacctgc ggcctgcttc ctcgacgaca cgcgccgtct cacgcgcgtc cgactcctct
     8641 tctgcaaggt acgagatgag cacgtccgcg ccctcgcgtg cgaaggcgac ggctacggcg
     8701 cgtccgatgc cgctgtcgcc cccggtgatg agagccgctt tgtttgcgag ccgcccgtgc
     8761 cccctgtagg agtcggctcc gtagtccggc ttcggggtca tctccgattc gatccccggc
     8821 acgtcctgcg gctgctcctc gaagggcggc ttcgccccct gctctttcgg gtcttccgtc
     8881 atcgtttccc tccccttgat tatgcgcccc gccacgcggc ggcggcctcg cctctattta
     8941 aacttcaact tcatccagac tatcgccgct ccgagggcga gcgttaccgc gttggtcacg
     9001 atgatggcga cttcaccctt gatgaagcca tagaccagcc agagcgatac gccgacgcag
     9061 aaggcgagga acatcgtcaa cgagacatct tccgccgact tcgtcttcca gatttttgtc
     9121 acctgcggca cgaacgacgc tgtcgtcaac ccgcccgccg ccaaccccaa ccaagtgatt
     9181 ccgtccatcg agtagtttcc ctcgcggcct ctccacagga tacttattcc gccgcttctc
     9241 ttgccgcttc aacgctctct ataattgtgg cttcatcttc gggaaaatct tcgggttctg
     9301 ctgtacgtta tggtacagcc gacgcagcgg cggggcattc gtgaggtaaa agttagaatc
     9361 ttgcgagggc ggcagtatat ttgacccgtc ccgttgcggt tcggccaaca cgtgccgttt
     9421 tcgcttcgca gcctcttcag ccagcatcac cctcaggcaa gtcgaatatg ttacggcgct
     9481 tcatttcact cataacgcaa cgccaccatc gggtctacct tcgtagccct tcgcgccggg
     9541 acgtaacagg cgatgagcgc gacgagtgtg agaagcgcag cgacgccggt gaaggtcagc
     9601 gggtcggtgg gtgagactcc atacaataga ctcgccattg ccctcgtccc gacgacggag
     9661 aggaacagac cgatgaggat acccgtcagg atcagagtca acccctcgaa gatgatcaag
     9721 cgcagcacct gactgccttt cgccccgagc gccatcctga tcccgatctc tttggttcga
     9781 ccgctgacgg cataagccgt cacgccgtag aggccgatgg aagccagccc ggttgccaga
     9841 aatcccaata agcccgccag ccagatcagc actttcgacg accccacgtg cccgtcaatt
     9901 atctcctcca gagttaaaac ttcgttggag ggcacgttcg catcgagcga tccggctacg
     9961 ctgtggatac tgttggtgat tgcctgccgg tcgccgtcgc tccgaacaat aagagagact
    10021 tgccccggcg tgtcttgctt gagcgggcga taagcgaagt agcgcggcct ctccgtaggc
    10081 cagaggtatt tgctgtctcg cgccacgccc accacctcca taaacctgtg cttcttatta
    10141 aacggatctc tttcgactaa ctgtttgccg agcgcgttgc cgtcgggcga gaggcgacga
    10201 gccagagtct cgttgacgat gatcacgtcg ggactcgatt gatcgtcgcg ctcggtaaaa
    10261 tcccgcccgc gcaaaagcgg tatctccaac gtctcaaaat atcctgtctc cacgctgttc
    10321 aaatagagcg agccgggctg gtcgaggacg tagagcgagg aactgttgtc tgtgaacacg
    10381 tcctgcgcgc cggaggctgg agactccccc gcaac
//
LOCUS       DASWNS010000235         4069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_243171, whole genome shotgun sequence.
ACCESSION   DASWNS010000235 DASWNS010000000
VERSION     DASWNS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243171"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1608
                     /locus_tag="VGB76_08125"
     CDS             13..1608
                     /locus_tag="VGB76_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEY0098907.1"
                     /translation="MELAKLYDTFGTPIDLMYVILSQGKHKIMPQPTFDDGHVQNRAV
                     IAAEQLTEEEFRGKIEEELKELQQSGTGKQTAEHKKAKPVYVALSRRTDTRSEFKGYE
                     TTSVEESKIVALLKGDEEVESLNEGEEGEVVLNHTPFYAESGGQVGDVGVFVGERANA
                     SVVDTYSPAQGLIVHKVKVEKGSLQVGDVISARVDVEKRDATRRNHTATHLMHAALRE
                     VLGTHVKQAGSVVAPNYLRFDFTHFQPLSRDEIAEIERLVNYHILRNEQVQTDELALE
                     EAMQTGAMALFGEKYAEKVRVLSVPGAEGVFSKELCGGTHVRSTGDIGLFKIVSDESI
                     ASGTRRIRAVTGWDALTRFRETEALVDQVASNLRATRGDLPATVERLQEELKRARRTA
                     DELRQKLASGGGAGGTSAGNGNDVREVAGIKVLAREASELDAAGLRQLSDTLLARVKS
                     GVVVLGRQGEGKASIIVRTSKDLHGRVPAGQVIRELAPIIGGRGGGKPDMAEGGGAQV
                     EKLAEALEASYEVVGRLMGANVN"
     gene            complement(2094..2462)
                     /locus_tag="VGB76_08130"
     CDS             complement(2094..2462)
                     /locus_tag="VGB76_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY0098908.1"
                     /translation="MKRLIFAFLTLLLAASVLQAGQNPNSSSAAATQESTTRKPAVKR
                     GAVFRATKEQIKQAQVILKGRGFFAGEDSGKLDAGTRAALRKYQEAESLKVTGTMNKA
                     TLEKMNITLTDRQKAWKPTT"
     gene            2913..3758
                     /locus_tag="VGB76_08135"
     CDS             2913..3758
                     /locus_tag="VGB76_08135"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0098909.1"
                     /translation="MKKSSFPCALILLAALFSCATHVSAQTGARYHHDNFDITGGVNI
                     VTEPTPAPPSAAPGKAKRSARQLTAFEQAHVRVKPTLYARPTNAPPMSARASLDGFTT
                     GDPKVDSYIADSSARHGVDPVLIYSIMHRESAFKKMAVSYKGARGLMQLMPATAARFG
                     VRNIFDPAQNIEAGTRYMRWLLNRFGGDVGLALAGYNAGEGAVDKYRGVPPYRETQEY
                     VKRISQRYALMRDPQTARRAPVLTRTPDAIASTASAETPAPQLYERNVYAVRLPDGKL
                     MLVTQ"
BASE COUNT          848 a         1123 c         1261 g          837 t
ORIGIN      
        1 tacattccta caatagaact cgccaaactt tatgacacct ttggaactcc gattgacttg
       61 atgtacgtga ttttgtccca aggcaagcac aaaatcatgc ctcaacctac atttgatgac
      121 gggcacgttc agaatcgcgc cgttatagcc gccgaacaat taacggagga agagttcagg
      181 ggaaaaatag aagaggaatt aaaagaactc cagcagagtg gaactggaaa acagacggcg
      241 gagcacaaaa aggcaaagcc cgtgtatgtc gcactctcac gacggacgga tacgcgctcc
      301 gagttcaaag gctacgagac gacgagcgtt gaggagtcaa agatcgtcgc gttgcttaaa
      361 ggcgacgagg aagttgaatc tttgaacgag ggcgaagagg gcgaggtcgt tttgaatcac
      421 acgccgtttt atgcggagag cggcgggcag gtgggagacg tgggcgtgtt cgtgggcgag
      481 cgtgcgaacg cgtcggtcgt tgatacttat tctccggcgc aggggttgat cgttcacaag
      541 gtcaaagttg aaaaggggag tttgcaggtt ggcgatgtaa tctccgcgcg ggttgacgtg
      601 gagaagcggg atgcgacgcg gcgcaatcat acggcgaccc acttgatgca cgcggcgttg
      661 cgcgaagtgt tggggacgca tgtgaagcag gccgggtcgg tcgtcgcgcc aaactatttg
      721 cgtttcgact tcacgcactt tcagccgctc tcgcgcgatg agatcgcgga gatcgaacgt
      781 ctcgtcaatt atcacatcct gcgcaacgag caggtgcaga cggacgaact cgcgctcgaa
      841 gaggcgatgc agacgggcgc gatggctctg ttcggcgaga agtacgcgga gaaggttcgc
      901 gtgttgagcg tgccgggcgc ggagggcgtc ttctcgaagg agttgtgcgg cggcacacac
      961 gtccgttcga cgggcgacat cggcctgttc aagatcgtga gcgacgaatc catcgcctcc
     1021 ggaacgagac gcatacgcgc cgtcacgggt tgggacgcgc tcacgcgctt ccgcgagacg
     1081 gaggctctcg tggatcaggt tgcgtcgaat ctgcgtgcga cgcgcggcga tcttcccgcc
     1141 acggtcgagc gtttgcagga agaactcaag cgcgcacgcc gcacggctga cgagttgaga
     1201 cagaagctcg caagcggggg aggcgcgggc ggcacgtctg cgggaaacgg caacgacgtg
     1261 cgcgaagtgg cgggcatcaa agtgctcgcg cgcgaggcga gcgaactcga cgcggcggga
     1321 ctcaggcagt tgtccgacac gctgctggcg cgtgtgaagt ccggcgtggt ggtgctcgga
     1381 cgacagggcg agggcaaggc ttcgatcatc gtgcgcacgt cgaaagattt gcacgggcgc
     1441 gtccccgccg gacaggtgat ccgcgaactc gcacccatca tcggcgggcg cggcggcggc
     1501 aagcccgaca tggccgaggg cggcggcgcg caggtggaga aacttgcgga ggctctggag
     1561 gcgagctacg aagttgtcgg gcgtctgatg ggagccaacg tcaactgagc aactaagacg
     1621 ttttgaaccg ccggaaagga agtttttagc gtcgttaatt caaacagaca aatcccctta
     1681 atcaaccgcc cgtcttctct ttcaaacccg ccgccctgaa acttcaggag gcgggtttga
     1741 aatttgggaa agaggaattg agagttggga atgtgagttt gaagattggg aagatgggtt
     1801 tggagattgg gaatgcgagt ttgaagattg gggatgtggg tttgtaagtt gggaagcggg
     1861 gcttaaagat tggggatgcc ggtttgtaag ttgggaacgt ctcctaaaca ttcaaacccg
     1921 cttccttgat taacctacgc acgggttgga atgtttagga agcgagtttg aattaacacc
     1981 gcgcgcggat aacttatgaa acgcttcgta aggggcggtt ggagatgacg cagacgagta
     2041 atcgtcaagt cacttccaac cgcctcaacg gtttttagct tgtccggcgc ggattacgtc
     2101 gtaggcttcc aagccttctg cctgtccgtg agcgtgatgt tcattttttc gagcgtggct
     2161 ttgttcatcg tgcctgtcac tttgaggctc tcggcttcct gatatttgcg gagcgcggcg
     2221 cgtgtgccgg cgtcgagttt gccgctatct tcgcccgcga aaaaaccgcg ccccttgagg
     2281 ataacttgcg cctgtttgat ctgttctttg gtggcgcgaa agactgcgcc gcgtttgacg
     2341 gcaggcttcc gcgtcgtgct ctcctgcgtc gccgccgcgc ttgaagagtt ggggttctgt
     2401 cccgcctgca acaccgacgc ggcgagcagc aaagtgagga atgcaaagat caatcttttc
     2461 atcgaagtct ccttccgaaa cgtgtgatgt atgaggcgtc cgggcgggta actcggcaag
     2521 tctaactcgg taagtctaaa gggatgaggc gtgaggtatc atacatgccc tgtcaacccc
     2581 ctctgaggcc gcgtgagtcg cgccgctgtc taacccgcgc ctcccctcga aactccttcg
     2641 agaactcacg ttaagagact ttttcgcgtt tttcccgcct tttagagtgg tttaaacttt
     2701 gttgttgccc gcgtttccgc ccgtatgcta ctctgctaat gcgttctcac ggacagcgaa
     2761 cgcgccccgc aacgaaaact ctccggcgaa cggttttcgc tcatcctctc aattctcaat
     2821 ctcaactgaa gacttgcggc gttggcggca gacatacgcc tctccacgca gattccgggc
     2881 atccacaggt attttccaga ttggcaagcg gtatgaaaaa gtcctccttt ccgtgcgcgc
     2941 tcatcctcct cgcagcactc ttctcctgcg ccacgcacgt ttcggcgcag acgggcgcgc
     3001 gttatcatca cgacaatttc gacatcacgg gcggcgtgaa catcgtcacg gaacccacgc
     3061 ccgcgcctcc ctccgccgct ccgggcaaag ctaaacgcag cgcacgtcaa ctgacggctt
     3121 tcgagcaagc gcacgtccgg gtgaaaccga cgctctacgc gcgaccgacg aacgcccctc
     3181 cgatgtctgc gcgcgcatcg ctcgacggct tcacgacggg cgatccgaaa gttgattcct
     3241 acatcgccga ttcgagcgcg cggcacggag tcgatccggt gctcatctat tcgatcatgc
     3301 accgcgagtc ggcgttcaag aagatggccg tctcctacaa gggcgcgcgt gggttgatgc
     3361 agttgatgcc cgcgacggcg gcgcgcttcg gcgtgcgcaa catcttcgac ccggcgcaga
     3421 atatcgaggc gggcacacgc tacatgcgct ggttgttgaa tcgtttcggc ggcgacgtgg
     3481 gactcgcgct cgcaggctat aacgcgggcg agggcgcggt tgacaagtac agaggcgtgc
     3541 ctccctaccg agagacgcag gaatatgtga agcgcatctc gcagcgttac gccttgatgc
     3601 gtgatccgca gaccgcgcgc cgcgcgcccg tcctgacgag gacgcccgac gccatcgcga
     3661 gcacggccag cgcggagacg cccgccccgc aactctacga gcgaaacgtc tatgccgtgc
     3721 gcctgcccga cgggaaattg atgctcgtca cgcagtaact tttcagagcg cgaagcgaaa
     3781 tcaaattaag cgagcggggc gtgtgtcggc gacacacgcc ccgctttcgt tttcctgatt
     3841 cgcagtagca atgactcaat gaaataacgt ttcctccgct gttcgatgaa agaccgcttc
     3901 attggccgtt cagcgtccgc gcttccgtcg ttatccctcg cgtttcctgc ctgtatggtc
     3961 ttaagcgacg ctcttaggta tgataaacgc gtgccggggc atcgcctccg gctgcaggct
     4021 aacgcaggaa actttggagt tgtcgccgaa cgtgtcgaac gccgcacag
//
LOCUS       DASWNS010000236         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_35307, whole genome shotgun sequence.
ACCESSION   DASWNS010000236 DASWNS010000000
VERSION     DASWNS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35307"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            479..3001
                     /locus_tag="VGB76_08140"
     CDS             479..3001
                     /locus_tag="VGB76_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0098910.1"
                     /translation="MGFDKAKAISAAEKYLAQGKIPSAITEYRRIVERDPHDYSALNT
                     LGDLYARTKKTQEAIECFQLVADHYREQGFTPKAIAVYKKISRLDQNLPIIAQRLAVL
                     YEQQGHFVEAREQYLIIAAAHARAGQMRQSLEALRRSADLEPNNVEIRLRLGEGFLTE
                     GLHEEAADAFTEAGERLLARGNAQSALDAYTQAHKIRPFNHAVLHGLVAAHAQLGASD
                     EAASVLEQAVSEEPQDVELRAMLSRAYLEAEDAPAAERATVALVELEASNYPQLFEVA
                     RLYLQRGDTGAAVGVLARITEPALAGRQEQKLLGLLNEALARDPEQIEALRLLLRIYT
                     WQRDDDRMRVTLERLAEAAQTHNLVGEERRALEHLVRLVPFDQTYHERLHELGDASPG
                     EAEATWYEPPVAEEIVAPSSPSSSAAPSSPSSSIATEVPTFENVFENSGGDAFSGAVE
                     STPLNVSTSSGASEFEWNTIAMPEHEEREGTGEPAAVTIDPNSSFADLNEDLEGRQQS
                     GWSSAAVNAKPIPFEAAELGVGSSFEEAGLAPEKGSGEGRVKMLLAQELESVDYYLAQ
                     GYTDIARDTLDMLERQYGANEEISQRRRQMPQEADSFGTLPLTSGLAADETSTEVSTE
                     FETFAPMEEEDAAEEVEIEEHMFISMVTEEDVAPVVETQPPVVFEAQPAPPAPQEAQG
                     FHHELADMLDEFRDEEEADDPASTGDYETHYNTGLAYREMGLVDQAVEELQAAIALTA
                     PLDGTPRYLQCCNLLGHCFMQKDMARPAAMWFKKGLDTPGHTEDEYQALRYELGTAYE
                     QMGDLERAIEVFSEVYGTDVNYRGVAAKLRDLQARKAVTSDK"
BASE COUNT          696 a         1028 c          949 g          594 t
ORIGIN      
        1 atcggcgtgc gcgccggtgt ggctcgccgg gtcttggagg ctcaaaactt tttgtctctc
       61 gtcgttcgta ggtatatgtg actggtaatt aaaagtgacg aaaacattca ccgcagagac
      121 atagcgaaag acgatgaagg aggaatgatg aacgcggaac gaaaactgaa aattctttag
      181 ttcatccttt cgcgttccgc cttccgcgtc tcttttctct gtgcccgctc cgcgccctcc
      241 gtctctgtgt cgagagtctt aacttctcaa tgacgggcgc ggcgaagtgg cggcgaagtg
      301 tttggcgaag ctgttaaaat gatgccgttg acactcgttg tcggcgcatg ttagcgtaaa
      361 atccgcccgc caccgaccga tagtgtgcga gccgggtgcg agacggttga gcgcgagatt
      421 ggcactttga aggcagccct ccacttacgc gcgacgcgga cacgaaacag ttttcctgat
      481 gggattcgat aaagcaaagg ctatcagtgc ggctgagaag tatcttgcgc aaggcaagat
      541 tccatcggct atcaccgagt atcgccgcat cgtcgagcgc gacccgcatg attatagcgc
      601 gctcaacacg ctcggcgacc tctacgcgcg caccaagaag acgcaggagg ccatcgagtg
      661 tttccaactc gtcgccgacc actaccgcga acagggcttt acgcccaaag ccatcgccgt
      721 ctataaaaag atttcccgcc tcgaccagaa cctccccatc atcgcccaac gcctcgccgt
      781 gctctacgaa cagcaggggc atttcgtcga agcgcgcgaa cagtatctca tcatcgccgc
      841 cgctcacgcg cgcgcggggc agatgcgaca atcgctggaa gctctccggc gcagcgccga
      901 tctcgaaccg aacaacgtcg agatacgtct gcgactcggc gaaggcttcc tcacggaagg
      961 cttgcacgaa gaggcggcag acgcgtttac agaagcgggc gaacgcctgc tcgcgcgcgg
     1021 caacgcgcag tccgccctcg acgcttacac gcaggcgcac aagatcaggc cgttcaacca
     1081 tgccgtgctg cacggcctcg tcgccgcgca tgcgcaactc ggcgcgtcgg acgaagcggc
     1141 ctccgtgctt gagcaggccg tgtcggaaga gccgcaggac gtggagttgc gcgcgatgct
     1201 ttcacgcgcc tatctggaag ccgaggacgc gcccgccgcc gagcgcgcga ccgtcgccct
     1261 cgtcgaactc gaagcgtcga actacccgca actcttcgaa gtggcgcgtc tctacttgca
     1321 gcgcggcgac acgggcgcgg cggtcggcgt gctcgcgcgc atcaccgaac ccgccctcgc
     1381 cggacgacaa gagcagaaac ttctcggact cttgaacgaa gccctcgcgc gcgaccccga
     1441 acagatcgaa gccctccgcc tgctcctgcg catctacacc tggcagcgcg acgacgaccg
     1501 catgcgcgtg acgctcgaac ggctcgccga agccgcgcag acccacaacc tcgtcggcga
     1561 agaacgccgc gcgctcgaac atctcgtgcg cctcgttccc ttcgaccaga cctaccacga
     1621 acgcctccac gaactcggcg acgcgtcccc gggcgaagcg gaagcgacct ggtacgaacc
     1681 gcccgtcgca gaggagatcg tcgcgccttc ttcgccgtcg tcctccgctg ccccttcttc
     1741 gccgtcctcc tccatcgcta cggaagtgcc gacctttgaa aacgtcttcg agaacagcgg
     1801 cggcgacgct ttttccggcg cggtcgaatc aactcccctg aacgtctcca cgagcagcgg
     1861 cgcgagcgag tttgaatgga acaccatcgc catgccggag cacgaagaac gggagggaac
     1921 cggagagcct gccgccgtca ccatcgatcc gaactcttcc tttgccgacc tcaacgaaga
     1981 cttggaaggc cggcaacagt cgggctggtc gtccgccgcc gtcaacgcga agccgatccc
     2041 tttcgaggcg gcggaactcg gcgtcggcag ttcatttgaa gaggcaggat tggctccgga
     2101 gaaggggtcg ggcgaagggc gcgtcaagat gctgctcgcg caggaactcg aaagcgtgga
     2161 ctattatctc gcgcagggct acacggacat cgcgcgcgat acgctcgaca tgctcgaacg
     2221 ccagtacggc gcgaatgaag agatcagcca gcggcgcagg caaatgccgc aggaagcgga
     2281 cagcttcggt actctccctc tcacctccgg cctcgctgcc gacgaaacct ccaccgaagt
     2341 ctccaccgaa tttgagactt tcgcgccgat ggaagaagaa gatgcggcgg aagaggtcga
     2401 gatcgaagag cacatgttca tctccatggt gacggaggaa gatgtcgcgc ccgtcgtcga
     2461 gacgcagccc ccggtcgtct ttgaggcgca gcccgccccg cccgcgccgc aggaggcgca
     2521 gggattccat cacgaactgg cggacatgtt ggacgagttt cgtgatgaag aggaggcgga
     2581 cgatcctgcc tccaccggag attacgagac gcactacaat acgggactcg cttatagaga
     2641 gatgggcttg gtcgatcaag ccgtggagga actgcaagcc gccatcgcgc tcaccgcgcc
     2701 cctcgacggc acgccgcgtt acctgcaatg ctgcaacctg ctcggccatt gcttcatgca
     2761 gaaagatatg gcgcgccccg ccgcgatgtg gttcaaaaaa gggctggaca cgcccggcca
     2821 caccgaagac gaatatcagg cgttgcgcta cgaactcggc acggcctacg aacagatggg
     2881 cgacctcgaa cgcgccatcg aagtcttctc cgaagtttac ggtacggatg tcaactaccg
     2941 gggcgtcgcc gccaaattgc gcgacctgca agcacgcaaa gcagtgacca gtgacaagtg
     3001 acgagtgaca agagcagaaa gtagatcgtg aatagatgaa gatggttttc tctattcacg
     3061 attcacgatt tacggctttt ctacgattta cgattcacgg cttttgttct atcttgtcac
     3121 ctgtcactcg tcactggtcg cttaagtata tgtcaggcag attaaccgtc atcttctata
     3181 tcatcctgtg tctcgaagcg ggcatcgtgt tgacgtttct gccgtgggtg cacccgttcg
     3241 gcttaagcga ttggggtgac aatttct
//
LOCUS       DASWNS010000237         8849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_138579, whole genome shotgun sequence.
ACCESSION   DASWNS010000237 DASWNS010000000
VERSION     DASWNS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138579"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VGB76_08145"
     CDS             complement(<1..1082)
                     /locus_tag="VGB76_08145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0098911.1"
                     /translation="MNAALAILFLIVGAGIALFVPKYGGAAILFGTALASLCGYAILR
                     SKVDRSFLIQLFVAALLIRMIVGTAIFAFQLQEFFGGDALSYDIYGWDVLLWWRGDAE
                     LVVTDDTVVWGMPYLVAAIYGVVGRNMLAVQFFNAICGAAAAIVVYKCAAHIFQNMSV
                     ARLAAVLVAFYPSLILWSSQGLKDAPIVLLLTITILATLKLGEKLSVKYGLILGGALL
                     GLLSLRFYIFYMVAAAITGAFIIGMKQVTAQNIARQFIVMIALGLSLTYLGVLRTASA
                     QLGAYGNLETMQRSRADLASSAQSGFAQDVDVSTTSGALSVIPVGMVYLLFAPFPWQM
                     VNLRQSITLPEMMLWWASFPLLVLGG"
     gene            complement(1165..2874)
                     /locus_tag="VGB76_08150"
     CDS             complement(1165..2874)
                     /locus_tag="VGB76_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0098912.1"
                     /translation="MYILGLTTLGDSAASLLYNGELVAAAEEERFTRVKHYSGFPYNA
                     VQFCLDHANIKIADVEHIGHYWKPWVLRHKAMQALKTSLISRDMFKARVDRGVAQVGD
                     SYLGMFKHPRRIRERFGESKFRFHFLEHHQCHAASTFFPSGFERAAIITWDGTGEDTT
                     TLFSQGEGNKLRVLKRIKLPHSLGQFYSAVTNFIGFDMFGGDEWKVMGLAAYGKPEYY
                     DFFRQRVLSTNGNNDFHLNIRVLDHHLAKHYQFGEEISKALGPPRQPDEDLTERHWNI
                     AASAQKVLEDTALHLIKGLHEMTGEENLCMAGGVAFNSVMNGRIMTESPFKNYFVQPA
                     AGDAGCSLGAALMVHHMLLKQPRKFRMEHAYYGPGFTSEECAAALTAAGLRFETLPDE
                     EMLPRVARMLADGAILGWFQGRMEFGPRALGNRSFIADPRRNDMRDLLNKKVKLREWF
                     RPLAPSMKEEASTEVFGRPHYDPFMVTVLHVAEEQQAAIPAVVHVDGTARPQTVSRRT
                     NPRYWKLIDEFARLTGVPMVLNTSFNIQEPIVCSAEDAIKTFQGANFDGLVLENHLVL
                     RRA"
     gene            complement(2875..4086)
                     /locus_tag="VGB76_08155"
     CDS             complement(2875..4086)
                     /locus_tag="VGB76_08155"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0098913.1"
                     /translation="MYTRLLNFLCCPQCNGDLEVFPLTASADAPAATPDSDAEISEGL
                     LYCRQAHWFPVVRGIPRMLPDALYEHWETVKALLPHPLPPMLHPVMETARAFAGRVNY
                     DQQTRENFSHEWDHHDLGGSTWGMKLSDRVEWFFLNPLRLPVAELKGKVMLDAGCGNG
                     SQSVAYTAFGLEVIAVDLSSGLEHGQAFRHRHEGANPGRVHFVQANLQQPPLKQATLD
                     IIHSAGVLHHTPDTLQTFRALRPLLKEGGTFYVWLYKYEKLVTPVVDTLRVVTTRVPP
                     RQFAKIADLMAAPFIGFCVAVNKLGLRSYPLMSRREAALALMDIFGAPYAYYHTFEEV
                     AGWYEAAGFKEVWPCNDDRRGFGVCGRLPATEKLERPQQQPASEGGAASSAPVGGATL
                     EAAASSQGGKL"
     gene            complement(4320..5312)
                     /locus_tag="VGB76_08160"
     CDS             complement(4320..5312)
                     /locus_tag="VGB76_08160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0098914.1"
                     /translation="MSALKETPEANAAATTTDAGNVAARAQDDPASAFDFRPTVCGAC
                     GSDRAKLLGWRGGDAHHGGAGVRTRIVRCDDCSHIYPNPMPYPARSLDDLYTDTDEYF
                     QGHDVEAKKQSALSKMAYFERALGRRGRYLDVACGRGESLWAARESGWEYEGVDASSA
                     YLEWAEANLGVRGRLGTLDEIKFPDNHFDAITMNAIIEHLYDPYATMREVFRILKPGG
                     LLSFDAPNEDGLYMRIGNLYMRAQGRDWVVTLAPTFPPYHVQGFNPRSLRRLLARVNF
                     EILQFTMGGHIWPPVGNPTWRKQAEYRAAQLIDWVDRRAGSGIYMDVIARKPQR"
     gene            complement(5309..7546)
                     /locus_tag="VGB76_08165"
     CDS             complement(5309..7546)
                     /locus_tag="VGB76_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEY0098915.1"
                     /translation="MKELLQSASGFNIVILCGGRGSGPLANQLLDQGARVTCLVNAYD
                     DGLSTGRLRYVFDELGPSDLRKNLLSLMNLDAPGYWSRYEVFAYRYPAEAERAADFRR
                     EIEDLCATGGAQLARGERRELLKMVARMPAGARERFVGYLRLFLNELVRREREEGAEF
                     SFSDCALGNCILLGAYFEHGRSWTQALRALEEVLQVRGRVEIASEENRHLFALCTDGR
                     VLASECAVITDADGDILELFLAKQPVAPLGLDKWVTQSGIETARERIRREVHAPTKAS
                     PGALEALERADVIIYGPGTFHSSLLPTLMIDGVCEAIRRSRATKIMVVNIVEEADTRG
                     ATGAELAQKITAQVGAGAYDYLIANVPHTHGGEGYFTFDEELLRGSSVKLATRDLEDR
                     TRPGRHRAPVLVQTILGIVRPEAAALARSTEEQPLVSIVMLAWNRKGEVEIGLNELRK
                     LTYPNIELIVVDNGSKDGTAQMVYEKFPEVTVVRLHDNTGMTGYNVGFATARGKYVAM
                     LDDDSHLAPESIETMVKLWEDESNKDVAVMAFRVMNPHLGSLVTHLWEERLVPTEPGR
                     EREITSFAACGAAIRRDVLDECGYFDDDFFIYATEDDLAIRLWDAGYKVIYEPRCLSY
                     HRESRQQRSWKRYGRGFRNAAWFNLKHLPWRYLPLVFIRNLFWLTLRGIRFRSFAYFF
                     YGLVGYFQGYLQCRTALRKRRPVKASVARFCLEDDWISRPIISTVRRIFADKRYILDK
                     RGVAP"
     gene            complement(7741..>8849)
                     /locus_tag="VGB76_08170"
     CDS             complement(7741..>8849)
                     /locus_tag="VGB76_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098916.1"
                     /translation="DAPLVRDGGARDGGQSLLRRRRDMNAPVSELDKLMDAAHVERVR
                     RAGALVLDVDDTLLARERAGGTGEETFRESAPAALLPELLRRGFNVCLITGHGWQQLQ
                     TRLIAPLIECWCEPRDAAADAATCIERLRVYANRGATKIVRDGERHVTDEVYGARHQL
                     RAEDMATLCELLETLGVEFNASYSARREWFRETFPRFDFATLPARVSEREGAVLVLRP
                     IPARRHAANGESDARAEVYRRGLQLLRRANLSDDYELAESGRSSIEITRRGVSKEAAM
                     RDLIAELATATGEDAARVEASLVYVGDEFFAGGNDAVIPRVFPRALCLSVASGHDGRE
                     TGAGVVSLAASAGAQGTAATGAFLAHVLNLSARV"
BASE COUNT         1694 a         2858 c         2694 g         1603 t
ORIGIN      
        1 cacccgccca agaccagcag cggaaagctc gcccaccaca acatcatctc cggcaaagtg
       61 atgctctggc gcaagttgac catctgccac ggaaagggcg cgaacaaaag atagaccatg
      121 ccgacgggga tcacgctgag cgcgccggaa gtggtcgaga catctacgtc ctgcgcgaag
      181 ccggattgtg ccgaagaggc caggtcggca cggctgcgct gcatcgtctc cagatttccg
      241 taagctccga gttgcgcgct cgccgtgcgc agcacgccca gataggtcag cgacagtccg
      301 agcgcgatca tcacgatgaa ctgccgcgcg atgttctgcg ccgtcacctg cttcatgccg
      361 atgatgaaag ctcccgtgat ggccgccgcc accatgtaga agatgtagaa gcgcaggctg
      421 agcaggccga gcagcgcgcc ccccaggatg aggccgtatt tgacgctcag cttctcgccg
      481 agtttgagcg tcgccagaat cgtgatcgtg agcaacaaga cgatgggcgc gtccttgagt
      541 ccctgcgacg accagaggat gagcgacggg tagaaggcca ccagcacggc ggccagacgc
      601 gcgaccgaca tgttctgaaa aatatgcgcc gcgcatttat agacgacgat cgccgccgcc
      661 gcgccgcaga tcgcgttgaa gaactgcacg gccagcatgt tgcgcccgac cactccgtag
      721 atggccgcca ccagatacgg catgccccac accaccgtat catccgtcac gaccagttcg
      781 gcatcgccac gccaccagag cagcacgtcc cagccgtaga tgtcgtagct gagcgcgtcg
      841 ccgccgaaaa actcctgcaa ctggaaagcg aagatggccg tgccgacgat catcctgatg
      901 agcagcgcgg cgacgaacag ttgaatcaga aaactccggt caaccttact tctcaggatc
      961 gcatagccgc acagcgacgc gagcgcggtg ccgaacagga tcgccgcgcc gccgtatttc
     1021 gggacgaaga gcgcgatgcc tgcgccgacg attaaaaaga gtatggcgag cgcggcgttc
     1081 attctcttaa aaaaccaacg actccggaga agtcagctcc ctcgaaacga tcaaagtcaa
     1141 aaccttcaag cccgactgcg cggtctaagc ccggcgcagc acgagatgat tttccaacac
     1201 cagtccgtcg aagttcgcgc cctgaaacgt cttgatggcg tcctccgcgc tgcacacgat
     1261 tggctcttga atattgaacg aggtgttgag caccatcggc acgcccgtca ggcgcgcaaa
     1321 ctcgtcaatc aacttccagt agcgcgggtt cgtccggcgg ctcaccgtct gcgggcgggc
     1381 tgtgccgtca acgtggacga ccgcagggat cgccgcctgc tgctcttcgg cgacgtgcag
     1441 gacagtgacc ataaacggat cgtaatgcgg gcgaccgaaa acttccgtgc ttgcttcttc
     1501 cttcatcgaa ggcgcgagcg ggcggaacca ttcgcgcagc ttaacttttt tgttgagcag
     1561 atcgcgcatg tcgttgcgtc gcgggtcggc gataaaggag cgattgccga gcgcgcgggg
     1621 tccaaactcc atgcgcccct gaaaccagcc gaggatcgcg ccgtctgcga gcatgcgcgc
     1681 cacgcgcggc agcatctctt cgtccggcag agtctcgaaa cgcaagcccg ccgccgtcaa
     1741 agccgccgcg cattcctcgg aggtgaagcc gggaccataa taagcgtgct ccatgcgaaa
     1801 cttgcgcggc tgtttgagca gcatgtggtg aaccatcaag gccgcgccga gcgagcaccc
     1861 ggcgtcgccc gccgccggtt gcacgaagta atttttgaac ggcgactcgg tcatgatgcg
     1921 accgttcatc accgagttga aggcgacgcc cccggccatg cacaggttct cttcgcccgt
     1981 catctcgtgc agacctttga tcaggtggag cgcggtgtct tccaagacct tctgcgcgct
     2041 cgcggcgatg ttccagtgcc gctcggtcaa atcctcgtcg ggctggcgcg gcggccccag
     2101 agccttgctg atctcttcgc caaactggta gtgcttcgcg aggtgatgat cgagcacgcg
     2161 gatgttcaag tgaaaatcgt tgttgccgtt ggtggagagc acgcgctggc ggaagaaatc
     2221 gtagtattcg ggtttgccgt aagcggccaa gcccatcacc ttccactcgt cgccgccgaa
     2281 catgtcgaag ccgatgaagt tcgtgaccgc cgaataaaac tggccgagcg aatgcggcaa
     2341 cttgatgcgc ttcaagacgc gcagtttgtt gccttcgccc tgcgagaaga gggttgtggt
     2401 gtcttcgccc gtgccgtccc aagtgatgat ggcggcgcgc tcgaagccgg agggaaagaa
     2461 ggtgctcgcc gcatggcact ggtgatgttc gagaaagtgg aagcgaaact tgctctcccc
     2521 gaaacgttcg cggatgcgac gcgggtgctt gaacatgccg aggtagctgt cgccgacctg
     2581 cgccacgcca cggtcaaccc gcgccttgaa catgtcgcgc gagatcagag aagtcttcaa
     2641 agcctgcatc gccttgtggc gcagcaccca gggcttccag tagtggccga tgtgttcgac
     2701 atcggcgatc ttgatgttcg cgtggtcgag gcaaaactgc acggcgttgt aggggaagcc
     2761 gctgtagtgc ttgacgcgcg tgaaacgctc ttcttccgca gcggccacga gttcgccgtt
     2821 gtagagaagc gaagcggcgg aatcgccgag cgtcgttaaa ccgagaatat acatttacaa
     2881 cttgcctccc tggctgctcg ccgccgcttc gagcgtcgcg ccgccgacgg gcgcagaaga
     2941 cgccgcgccg ccttcggacg cgggctgctg ctgcgggcgt tccaactttt ccgtggcggg
     3001 cagacgcccg cagacgccga agccgcgccg gtcgtcgttg caaggccaga cttccttgaa
     3061 ccctgcggcc tcgtaccacc cggcgacctc ttcaaaagta tgatagtacg cgtagggcgc
     3121 gccgaagatg tccatcaggg cgagcgcggc ctcgcgccgg ctcatcagtg gataactgcg
     3181 caagcccagt ttgttgaccg ccacgcagaa gcctatgaag ggcgcggcca tcaagtctgc
     3241 gattttggcg aattgacgcg gaggcacacg cgtcgtcacc acgcgtagag tgtcaactac
     3301 gggcgtcacc agtttctcgt atttatagag ccagacgtag aacgtgccgc cctctttcag
     3361 cagcgggcgc aacgcgcgga aggtctgcaa agtgtcgggc gtatggtgca ggacgcccgc
     3421 cgaatggatg atgtcgagcg tcgcctgctt gaggggcggt tgctgcaagt tggcctgcac
     3481 gaaatggacg cgcccgggat ttgcgccctc gtgcctgtgc ctgaaagcct gcccgtgctc
     3541 aaggccggaa ctgagatcaa ccgcgatcac ttccagcccg aacgccgtgt aggcgaccga
     3601 ctggctgccg ttgccgcagc ccgcgtcgag catcaccttg cctttcagtt cggcgacggg
     3661 gaggcgcaga gggttcagga agaaccactc cacgcggtcg gagagtttca tgccccacgt
     3721 cgagccgccg aggtcgtgat ggtcccattc gtggctgaaa ttttcccgcg tctgctggtc
     3781 gtagttgacg cgtccggcga aggctcgcgc cgtttccatc accggatgca gcatgggcgg
     3841 gagcgggtgc ggaagcaacg ccttcacggt ttcccagtgc tcgtagagcg cgtcgggcag
     3901 catgcgcgga atccctcgca ccaccggaaa ccagtgcgct tgacggcagt aaagcaaacc
     3961 ttctgatatt tctgcgtcgg aatccggggt ggcggcggga gcgtcggcgg aggcggtgag
     4021 ggggaaaact tccaagtctc cgttgcactg tgggcaacac aggaaattca acaaacgtgt
     4081 gtacatagag aaaacaaaag caactcaaaa tactgacggc gcgaacgtgt ctccttcgcg
     4141 ccggaccaac tcggcctgac agaccacttc agcaaacgac ccaaaagcca gactatcagc
     4201 gaccgcctcg ctctcatacg acaaaccgga aaatatttta tttacgcgcc ggaatcccga
     4261 cttgtttaat gcttgaacct accagaattc cgcccaaaag taaaaacgcg gttcttcatt
     4321 taacgctgcg gtttacgcgc gatgacatcc atataaatgc ccgacccggc gcgacggtca
     4381 acccagtcga tcagttgggc ggcgcggtat tcggcctgtt tgcgccacgt cgggttgccc
     4441 acgggcggcc agatgtgccc gcccatcgtg aactgtagaa tctcgaaatt cacgcgcgcc
     4501 agcagtcgcc tcagcgaacg cgggttaaag ccctgcacgt gatagggcgg gaaagtgggg
     4561 gcgagcgtca ccacccagtc gcgcccctgc gcgcgcatgt agagattgcc gatgcgcatg
     4621 tagaggccgt cctcgttcgg cgcgtcgaac gagagcaggc cgcccggttt gaggatgcgg
     4681 aagacttcgc gcatcgtggc gtaagggtcg tacaggtgct cgatgatggc gttcatggtg
     4741 atggcgtcga agtgattgtc gggaaacttg atctcgtcga gcgtgccgag ccgtccgcgc
     4801 acgccgaggt ttgcctccgc ccactccaga tacgcgctcg acgcatccac gccttcatac
     4861 tcccacccgc tctcgcgcgc tgcccacagg ctctcgccgc gcccgcacgc cacgtcgaga
     4921 taacgaccgc gacgcccaag cgcgcgttca aagtaggcca tcttcgagag cgcgctctgc
     4981 ttcttcgcct ctacgtcgtg gccttgaaag tattcgtcgg tgtccgtgta gagatcgtcg
     5041 aggctgcgcg cgggatacgg catcgggttc ggatagatgt gcgagcagtc gtcgcaacga
     5101 acgatgcgcg tgcgaactcc cgcgccgccg tgatgcgcgt cgccgccgcg ccagccgagc
     5161 aacttcgcgc ggtcgctccc gcacgcgccg cacacggtcg gtcgaaagtc gaaagccgac
     5221 gccggatcgt cttgcgcccg cgctgcgacg tttccggcgt ccgtagtcgt cgccgccgcg
     5281 tttgcctccg gcgtctcctt caaggcactc atggcgcgac tcccctcttg tccagaatgt
     5341 aacgcttgtc tgcgaagatg cgccggacgg tgctgatgat cgggcggctg atccaatcgt
     5401 cttccagaca gaaacgcgcg accgacgcct tcaccgggcg acgtttcctg agcgcggtgc
     5461 ggcattgcag gtagccctga aaataaccga ccagcccgta gaagaagtat gcgaaggagc
     5521 ggaagcgtat gccgcgcaaa gtcagccaga acaggttgcg tatgaagacg agcggcagat
     5581 atcgccacgg cagatgtttg aggttgaacc acgcggcgtt gcgaaagccg cgcccgtagc
     5641 gtttccacga acgctgctgg cgactctcgc ggtgatacga caggcagcgc ggctcgtaga
     5701 tgaccttgta gcccgcgtcc cagagacgta tggcgagatc gtcttcggtg gcgtagatga
     5761 agaagtcgtc atcgaagtag ccgcactcgt ccaaaacgtc gcgccggatg gccgcgccgc
     5821 aggctgcgaa actcgtgatc tcgcgctcgc gccccggctc ggtcggcacc agacgctctt
     5881 cccacaggtg cgtgacgaga cttcccagat gcgggttcat cacgcgaaaa gccattaccg
     5941 ctacgtcctt attcgactcg tcttcccaca gcttcaccat cgtctcgatt gactccggcg
     6001 cgaggtgcga atcgtcgtcg agcatggcga catacttgcc gcgcgccgtc gcaaagccga
     6061 cgttgtagcc ggtcatgccc gtgttgtcgt gcagccgcac gaccgtcact tcgggaaact
     6121 tttcatagac catctgcgcc gtcccgtcct tcgagccgtt gtccacgacg atcaactcga
     6181 tgttcgggta ggtcagtttc cgcaactcat tcaaaccgat ctcgacctcg cccttgcgat
     6241 tccacgcgag catcacgatg ctcacgagcg gttgttcctc cgtgctgcgg gcgagcgcgg
     6301 cggcttcggg gcggacgatg ccgaggatgg tctgcacgag cacgggcgcg cgatgcctgc
     6361 ccggtcgcgt gcggtcttcc agatcgcgcg tggcgagttt gacgctcgac ccccgcagca
     6421 gctcttcgtc aaacgtgaaa taaccttcgc cgccgtgtgt gtgcggcacg ttcgcgatca
     6481 gataatcgta tgcgcccgca cccacctgcg cagtgatctt ctgcgccagt tccgcgcccg
     6541 tcgcgccgcg cgtgtccgcc tcttcgacga tgttgacgac catgatcttc gtcgcgcggc
     6601 tgcggcggat cgcttcgcac acgccgtcga tcatcagcgt cggcaggagc gacgaatgaa
     6661 acgtgcccgg accatagatg atcacatcgg cgcgctccaa cgcttcgagc gcgccggggc
     6721 tggccttcgt cggcgcatga acttcgcggc ggatgcgctc gcgcgccgtc tcaatgccgg
     6781 actgcgtcac ccacttatcc agaccgagcg gcgcgaccgg ctgtttggcg agaaagagtt
     6841 cgaggatgtc gccgtcggcg tccgtgatga cggcgcactc gctggcgagc acgcgcccgt
     6901 ccgtgcagag cgcgaagaga tgccggttct cttccgacgc gatctcgacg cgcccgcgca
     6961 cctgtaagac ttcttcgagc gcacgcaaag cctgcgtcca actgcgcccg tgctcgaaat
     7021 acgcgccgag caggatgcag ttgccgagcg cgcagtcgct aaaggagaac tccgcgccct
     7081 cttcgcgctc gcgcctgacg agttcgttca ggaagagacg taggtagccg acgaaacgct
     7141 cgcgcgcccc cgcaggcatg cgcgccacca tcttcaacag ttcgcgtcgc tcgccgcgcg
     7201 caagctgtgc gccgcccgtc gcgcagaggt cttcgatctc gcgccggaaa tctgcggcgc
     7261 gctccgcttc cgccgggtaa cggtaggcga agacttcgta gcgcgaccag tagcccggcg
     7321 cgtcgaggtt catcagggag agcaggtttt tgcgcaggtc ggaggggccg agttcgtcga
     7381 agacgtagcg gagccgcccc gtgctcaacc cgtcgtcgta agcgttgacg agacaggtga
     7441 cacgcgcgcc ctgatcgagc agttgattgg cgagcggacc gctgccgcgc ccgccacaca
     7501 aaatgacgat gttgaacccg ctggccgatt gaagcaattc tttcatctta aaaactatgc
     7561 tctgactcca acgctccgag caatcactca atccttcgcg cccctccggc ctcttctctg
     7621 cgcctccggc gtgaaattgg tttctcgccc gacgggcgaa gttaagagtt aaggagcaaa
     7681 gtagtctctg agtctcactc taaaattatc cttcttcacg aaggcgttaa taatgcacaa
     7741 tcaaactcgc gccgacaagt tgagaacatg tgcgaggaac gctccggtgg ctgccgtgcc
     7801 ctgcgcgccc gcgcttgcgg cgagcgacac gacgcccgcc cccgtctccc gcccgtcgtg
     7861 cccggatgcc accgacaggc agagcgcacg cgggaagacg cgcgggatca cggcgtcgtt
     7921 gccgcccgcg aaaaattcat cgccgacgta gacgagcgac gcttccaccc gcgccgcgtc
     7981 ttcgcccgtg gccgtcgcca gttccgcgat cagatcgcgc atggccgctt ctttcgagac
     8041 gccgcgccgc gtgatctcga tgctgctgcg cccgctctcg gccagttcgt agtcgtcgct
     8101 cagattcgcg cgccgcaaca attgaagtcc gcgccggtaa acttccgcgc gtgcatccga
     8161 ctctccgttc gccgcgtgcc gccgcgccgg gatgggacgc agcaccagca ccgcgccttc
     8221 gcgctccgac acgcgcgcgg gcagcgtcgc aaaatcgaaa cgcggaaacg tctctcgaaa
     8281 ccactcgcgg cgtgcgctgt acgaagcgtt aaactccacg cccagagttt cgagtagttc
     8341 gcagagcgtc gccatatcct ccgcgcgcag ttgatgacgc gccccgtaaa cctcgtccgt
     8401 cacgtgccgc tcgccgtccc ggacgatctt cgtcgcgccg cgattggcat agacgcgcag
     8461 acgctcgatg cacgtcgctg cgtcggcggc agcgtcgcgc ggctcgcacc agcattcgat
     8521 gagcggcgcg atcaggcgcg tctgtaactg ctgccagccg tgccccgtga tgagacacac
     8581 gttgaagccg cgacgtaaaa gttcgggcag cagggcggcg ggcgcgctct ccctgaaagt
     8641 ctcctcgccc gtgccgcccg cgcgctcgcg cgccagcagc gtgtcgtcaa cgtcgagcac
     8701 gagcgcgccc gcgcggcgca cgcgttcgac gtgcgcggcg tccatcaact tgtcgagttc
     8761 ggacacaggc gcgttcatat ctcttcgtcg ccggagcaga ctttgcccgc cgtctcgcgc
     8821 gccgccgtct cgcaccagag gcgcatctc
//
LOCUS       DASWNS010000238         4677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_398620, whole genome shotgun sequence.
ACCESSION   DASWNS010000238 DASWNS010000000
VERSION     DASWNS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398620"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..1259)
                     /locus_tag="VGB76_08175"
     CDS             complement(348..1259)
                     /locus_tag="VGB76_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098917.1"
                     /translation="MSAPVKKRDPRVGLRTSATFATIFTILGHTVLGFEQPVAQVFVA
                     LGAGYACALFFEWVDARANGIAPAYAGGGFRKFVDFLLPAHMTSITMSFLLYFNQRLW
                     IMALSVALALGSKYVLRVRQNGRLQHFMNPSNFGIAIVLIAYQWTGVLPWAFTIDIYG
                     VWDWLLPLIIVLLGFRLNLLFTGRLPTVATWLSTFILLGIVRSWILSTPIAAELVVLT
                     GIPMVLFTLYMITDPQTSPSRLRSQILFGAGIAFAYHVLLMMHVQYMMFYSVALVCAI
                     RGLCLFALSLREPSVERVAVPVTAPGV"
     gene            complement(1256..3217)
                     /locus_tag="VGB76_08180"
     CDS             complement(1256..3217)
                     /locus_tag="VGB76_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0098918.1"
                     /translation="MLAVFVVVGALSVFAKLPTPPPAETARLAARFQFAWQTLPPAPI
                     PAGGVVFPVNKTAKHMQFYFYQVGASAALGDLDGEGLPNDLCLTDVRAKALTVSPVPG
                     TGDRYAPFTVDFGSLVDRVTVYPSVCRIADMNEDGLADIFVAFYGRPPLLLLHRASGD
                     AKSPAPLSMASFSISELIPGLSERWWTATATFADMDGDGHQDIIVGNYYPDGAQITDA
                     NSDRPFEMNSDFSRARNGGKNRLFLYTQSASGATPATLYRDAGDVFPNNGAYAWTLAL
                     GAADLDRDGLSDLYVANDFGPDQLLWNRSRPGAVKFQELYGSRGFFRPPSMVMGHDSF
                     KGMSIDFGDVNHDGIFDMYVSNIGSPFALQEGHFLWTSTGQMDRLAQGGAPWIDRADD
                     VGVSHSAWAWDARFEDFDNDGVLEIVQATGLVKGTDNKWPDLSQVGAGNDAFVKYQAS
                     WPNFLPGSAEVDGSFPNPFWVLGSTGLYDDLSTTLFPGLTPATRGIAVADVDGNGYSE
                     MVYANFWEDSVYVKNQSTGNRFLGLHLLLPVASADGSAKPPEGTRVHEGHPARREGTP
                     AIGAFVEAQLPDGRRLIRQVDGGNGHSGQRSPEVLFGIGRTPANEIPVRITWRDLRGV
                     LHHETLSLAPGYHTVVLAAQGEKPQGEIQ"
     gene            complement(3327..>4677)
                     /locus_tag="VGB76_08185"
     CDS             complement(3327..>4677)
                     /locus_tag="VGB76_08185"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0098919.1"
                     /translation="KGTGIRIDGAPGMVDFDGFIKDLVISLALTEASPALLPDSRFNR
                     FARKVLKENYSLENALKLKAEVAKFLLSKKREQDQEKESDRRRGETPTKGGFGRIDAL
                     GAGGNRLYRQLGVKNLRTLNSPVKAISLWNTHRYNWVQSNGSLRQPMARNIIEALAVN
                     ASLIFPGDPAKNDRYLSSARLNNMFEMETMIQRFKAPIWPEKVFGKINQDAVKRGEVH
                     YQKHCASCHTPQMESHPESGDPVAVKNNKTFFVLKLVPLDEIKTDPTDAVNFAERRLD
                     ASSIKDDAGKPMGNDVPGPVVIDMVLSGIINRRYQELKLPVEQQEEWNGYRANLLRAC
                     KAYPARPLAGVWANSPYLHNGSVHSLYQLLLPPAERDKTFFTGDVEFDPVNVGYLTTQ
                     LPGGFKFDTTLTGNSNSGHEYGISLTHEERMDLIEYLKDLKFPEKDYPLVAPQASCP"
BASE COUNT          912 a         1356 c         1504 g          905 t
ORIGIN      
        1 taggaaaggc cgacggcggg aacagttcca gagaggagag cagagcccac acaccgatgc
       61 ctgcgaggaa gcaggcggtc gagagcagaa aggctttggc gtaagaggtt cgttgcgcgg
      121 tcttcatctc aacattctat ctacttcaat ctcgcggctt tcgcaagtca tccgtcaagc
      181 gcggtatccg gttttcagct taactctccg tgccccttct gtgtcctctg tgagtctgtg
      241 gtgagtgctt gattaactaa gattcgccac agactcacag aggacacaga aggggcacgg
      301 agtataaatc agatgagtcg agcacccgtc ggtctacacg gaacttctca gacccccgga
      361 gccgtgacag gcacggcgac ccgctcgaca gacggctcgc gcagactgag cgcgaagaga
      421 cacagcccgc gaatcgcgca gaccaacgcc acgctgtaga acatcatgta ctgcacgtgc
      481 atcatcaaca gcacatgata agcgaacgcg atccccgcgc cgaagagtat ctggcttctg
      541 aggcgcgaag gcgaggtctg cgggtctgtg atcatgtaga gggtgaagag caccatcggg
      601 atgcccgtga gcacgacgag ttcggcggcg atgggcgtgc tcagtatcca cgaacggacg
      661 atgccgagca ggatgaaagt gctgagccac gtggcgaccg tcgggaggcg gcctgtgaac
      721 aacaggttca gcctgaaccc gagcagcacg atgatgagcg gcagcagcca atcccagaca
      781 ccgtaaatgt cgatggtaaa agcccacggc agcacgcccg tccactggta ggcgatgagc
      841 acgatagcga tcccgaagtt cgacgggttc atgaagtgct ggaggcgtcc gttctgacgc
      901 accctgagca cgtacttcga gccgagcgcg agcgcgacgg acaaagccat gatccagagc
      961 cgctggttga agtagagcag aaaggacatc gtgatcgatg tcatgtgcgc cgggagcagg
     1021 aagtctacga acttgcgaaa gccgccgcca gcgtaggcag gggcgatgcc gttggcgcgc
     1081 gcgtcaaccc actcgaagaa gagcgcgcac gcgtagcccg cgccgagcgc gacgaagacc
     1141 tgcgcgacgg gctgctcaaa gccgagcaca gtatggccga ggatcgtgaa gatcgtggcg
     1201 aaggtagcgg aggtgcgtag accgacgcgt gggtcgcgtt tcttaacggg cgcgctcatt
     1261 gaatttctcc ttgtggtttt tctccctgtg cggcgagcac aacggtgtga tagccggggg
     1321 cgagcgaaag cgtctcgtga tgaagcacgc cacgtagatc gcgccaggtg atgcgcaccg
     1381 ggatttcatt cgcgggggtg cgcccgatgc cgaagagaac ttccggactc ctttgcccgg
     1441 aatgcccgtt accgccgtcc acctgccgta tgagacgccg cccgtccgga agttgggctt
     1501 cgacgaaggc tccaatcgcg ggcgtgcctt cccgccgtgc cggatgccct tcgtggacgc
     1561 gcgtgccttc cggcggcttc gcggagccgt cggctgacgc cacgggaagc agcaggtgca
     1621 ggccgaggaa acggtttccc gtagactggt ttttgacgta gaccgagtct tcccagaaat
     1681 tcgcgtagac catctcggag tagccgttgc cgtccacatc ggcaaccgcg atgccgcgcg
     1741 tcgccggggt caggccggga aagagtgtgg tcgagaggtc gtcgtacagc cccgtgctgc
     1801 cgagaaccca gaagggattc gggaacgagc cgtcaacttc cgccgagccg gggaggaagt
     1861 tcggccaaga cgcctggtac ttcacaaacg catcgttgcc cgcgccgacc tgagagagat
     1921 caggccactt gttgtccgtg cccttgacca agcccgtcgc ctgcacgatc tcaagcacgc
     1981 cgtcgttgtc gaaatcctcg aagcgcgcat cccaagccca cgcgctgtgt gagacgccga
     2041 catcgtccgc gcggtcgatc cacggcgcgc cgccctgcgc cagacgatcc atctgcccag
     2101 tgctggtcca gaggaaatgt ccctcctgca aggcgaaggg cgagccgatg ttgctgacgt
     2161 acatgtcgaa aatgccgtcg tggttgacat cgccgaaatc tatgctcata cccttgaagg
     2221 agtcgtgtcc catgaccatt gagggcggac ggaagaagcc acggcttcca tagagttcct
     2281 gaaatttgac cgcgccgggg cgcgagcgat tccacaggag ttggtcgggt ccgaagtcgt
     2341 tggcgacgta gaggtctgag agtccgtcgc ggtcgaggtc agccgcaccg agggcgagcg
     2401 tccaggcata cgcgccgttg ttcgggaaca cgtcgcccgc atcgcggtag agcgtggccg
     2461 gggtcgcgcc cgaggcgctc tgtgtgtaga gaaagaggcg attcttcccg ccgttgcgcg
     2521 cccgcgagaa atcgctgttc atctcgaagg gacgatccga gttggcgtct gtgatctgcg
     2581 cgccgtccgg gtagtagttg ccgacgatga tgtcctgatg gccgtcgccg tccatgtcgg
     2641 cgaaggtcgc cgtcgccgtc caccaacgct cggagaggcc ggggatgagt tcgctgatgg
     2701 agaacgacgc catcgagagc ggggcgggcg acttcgcatc gccggaggcg cgatgtagca
     2761 gcaggagcgg ggggcggcca tagaaggcga cgaagatgtc cgcgaggccg tcctcgttca
     2821 tgtccgcgat gcgacagacg ctcggataga ccgtcacgcg atctacgagg ctgccgaagt
     2881 caaccgtgaa cggcgcgtag cggtcgcccg ttccgggcac ggggctgacc gtcaacgcct
     2941 tcgcgcggac atctgtgagg cacaggtcgt tcgggagtcc ctcgccgtcg aggtcgccga
     3001 gcgcggctga cgcgccgacc tgataaaagt agaactgcat gtgtttcgcc gtcttgttca
     3061 cggggaagac gacgcctccg gcggggatgg gagccggggg gagcgtctgc cacgcaaact
     3121 gaaagcgcgc tgcgagtcgc gccgtctcgg cgggcggcgg cgttggaagt ttggcgaaga
     3181 ccgagagcgc gcccactacg acgaaaactg cgagcatgac ttgaaagtta tgttttcttg
     3241 ctccaacctt tttgtccgga atcacgattc ctccttatca gccggtgtcc tctcaagcgg
     3301 gcggctgtct aacttatgat tactacttac gggcaactcg cctgcggagc gacgagcggg
     3361 taatccttct cgggaaactt aaggtctttc agatactcta tgaggtccat gcgctcctcg
     3421 tgggtaagcg agatgccgta ttcatgcccg gagttcgagt taccggtgag cgtcgtgtcg
     3481 aacttaaatc cgccggggag ttgcgtggtc agatagccga cgttgaccgg gtcgaattcc
     3541 acgtcgcccg tgaagaacgt cttgtcgcgc tcagccgggg ggagcagcag ttggtagagg
     3601 ctgtggacgg agccgttgtg gagatagggc gagttagccc agactccggc cagtggccgc
     3661 gccggatatg ctttacaggc acgcaggaga ttggcgcggt agccgttcca ttcctcctgt
     3721 tgttcgacgg ggagtttgag ttcctgatag cgtcgattga tgatgccgga gagcaccatg
     3781 tcgatcacga cgggacccgg tacgtcgttg cccatcggct tgcctgcatc gtccttgatg
     3841 ctcgaagcgt ccagcctgcg ttcggcgaaa ttcacggcgt ccgtcgggtc ggtcttgatc
     3901 tcgtcgagcg gcaccagttt caacacgaag aacgtcttat tgtttttgac cgccacgggg
     3961 tcgccggatt ccggatgact ttccatttgc ggcgtgtggc aggaggcaca gtgtttctgg
     4021 taatgcactt cgccccgctt gaccgcgtcc tgattgatct tgccaaacac tttctccggc
     4081 cagatcgggg ctttgaacct ctgaatcata gtctccatct cgaacatgtt gttgagccgg
     4141 gcgctggaga ggtagcgatc attcttggcc gggtcgcccg gaaatatcag gctcgcgtta
     4201 acggccagag cctcgatgat gttccgcgcc atcggctggc ggagcgagcc gttcgactgc
     4261 acccagttgt agcgatgcgt gttccagagc gagatggcct tgacgggaga gttcagagtt
     4321 cggagattct tgacgccgag ttgacggtat aaacggttgc cgcccgctcc gagcgcatcg
     4381 atcctgccga agcctccttt ggtcggcgtt tcgccgcgtc gcctgtcgct ctccttctcc
     4441 tggtcctgtt ccctcttttt cgacaacagg aacttcgcca cctccgcttt cagtttcaac
     4501 gcgttctcca gactgtagtt ctctttcagc accttgcggg cgaagcgatt gaatctggaa
     4561 tccggcagca gcgcgggaga tgcctcggtg agagcaaggc tgatgaccaa atctttgata
     4621 aacccgtcga agtcaaccat gcccggcgcg ccgtcgatgc ggatgcccgt gccctta
//
LOCUS       DASWNS010000239         6511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_294884, whole genome shotgun sequence.
ACCESSION   DASWNS010000239 DASWNS010000000
VERSION     DASWNS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294884"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..275)
                     /locus_tag="VGB76_08190"
     CDS             complement(30..275)
                     /locus_tag="VGB76_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0098920.1"
                     /translation="MNGARRSERRVAVRCIRRRRRSKNGRAANTRPTKRGADERSRAF
                     DIDGNAAILKVSFDEPGTRFSGYVSMLKLCVEWKIVN"
     gene            complement(253..522)
                     /locus_tag="VGB76_08195"
     CDS             complement(253..522)
                     /locus_tag="VGB76_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY0098921.1"
                     /translation="MRLDQFLRASRLVLRRTVAQELCEAGAVVVNGAVARSSRAVRVG
                     DEITLARRANFLTVRVLSVPTARQTSKQEAATLYEVVRDERREEE"
     gene            complement(619..1788)
                     /locus_tag="VGB76_08200"
     CDS             complement(619..1788)
                     /locus_tag="VGB76_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0098922.1"
                     /translation="MKLKFLKRARRVGASALLLAALLAPNAASLRAQEEGVPRVIDEV
                     IAQVNSEVITLSTLKREMAEAVEAIKGQRGITEQQASEEITKRRPEIIATLINEQLLV
                     QKGKELGMTEEVEAEVNRRMLDVMKKEGFKTLAQLEQAMRESKIDPASVRATMRTEIM
                     KTWVLGREVDQKVFYSLSETEVKNYYETNKEKFRKPETVKLSEIYLSTVGKDPKEVRE
                     RADKLVAQLRAGADFKAIAMVQSEREVDGKRIAPENKGELGTYAMSDITKEEVSNAIK
                     GVKTVGGVTNPLDSDNGIIILRVDERTAGGEPAFDENRVREALTYTRLDKERKTYIDN
                     LRRDAYIQLAKDYRDEVLPLLKSDAEKATPAAVAATAPAAKKDDKKKSDNGNKKP"
     gene            complement(2037..5759)
                     /gene="mfd"
                     /locus_tag="VGB76_08205"
     CDS             complement(2037..5759)
                     /gene="mfd"
                     /locus_tag="VGB76_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY0098923.1"
                     /translation="MSSTVQKSSPVEGALRRVLEGEELARLLAEVRGGARVVSVSGLT
                     SGAARALVLAALQRETGKRLAVVVQASRDIEQWERDLCFWSEALRQRRDGEEQGGAGR
                     RASVLTLPASESDPYAGASPHPETLERRALTLWRLARGEGGFVLLTARALARRTIAPQ
                     QLLATGASLRRDADYAPEDLIELLLASGYVREDPIGAVGEFSLRGGILDVWSPGQDAP
                     VRVEFFGDTVDSIREFDPETQLSIAQRQEIEIVPMREYALGADDFRLWAELARERWPD
                     ERYARALRDRTVHAEEGETFAGWEWLVPLVHSTHASAFDYLQRDTIFVADEPAALETF
                     LGNTYETLNARYAETEGADEVALRPEELYLTAEELRAGVAGVPRVELRTLGRAAATTD
                     ERFTGEVEAASVTIGRTRANAAPLFLFPAVERAPEVEWNTRAARRYHGRIADLAADVR
                     RVQAQADARVLFAMPSAGLAERVGEMLAEYNVAARVVAAGEHGGALEDGQALVTVGRL
                     SGGFELPSAGLVVHVEHDLFDEAHDGASVEHRAPGAQGQGAGAASGTGAQRTRRKRSK
                     TAAFLSDFRDLKVGDFVVHIDHGIARFGGLQTLDLSGRKSEFMLLFYAEDAKLYVPVE
                     RLDLVQRYSSAEGHEPTLDRLGGLGWQKTKAKAKREMRDMADELLRLYAERKLVGGYA
                     YAADSPWQREFEDGFEYVPTPDQETAIEDVKTDMEATTPMDRLLCGDVGYGKTEVAMR
                     AAFKAVMESKQVAVLTPTTVLAYQHFETFSARFAAFPVKIELLSRFRTPKEQKQVVRA
                     VEAGEVDILIGTHRILSKDLAFRDLGLVVVDEEQRFGVAHKERLKQLKKKVDVLTLSA
                     TPIPRTLNMSLMGLRDMSVIETPPRDRLAIQTQVVQFSESVIKSGIELELGRGGQVFF
                     IHNRVETIETIAALVQRLVPQARVCVAHGQMNEKEMESVMLDFMAFKYDVLVATTIIE
                     NGIDIPRANTIIINRADHYGLAQLYQLRGRVGRSNRRAYAYLLIPSEQELTPIARRRL
                     AAIREFSDLGAGFRVAALDLELRGAGNLLGGQQSGHMDALGFDLYTQMLERTVSELRG
                     EDVQDETSVSLNLGVDVAISEDYIPDMGQRLRTYKRIASARDDETLRQIRAETEDRYG
                     RIPASVERLLAYARLRRTAEAVGVLSLDRTPTGVAIKLSEKARVAPEKLLALVAERTA
                     ATFTPNGVLRVDLSEDETEDVIETAHGLLLRISRAE"
     gene            complement(5888..6376)
                     /gene="rfaE2"
                     /locus_tag="VGB76_08210"
     CDS             complement(5888..6376)
                     /gene="rfaE2"
                     /locus_tag="VGB76_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEY0098924.1"
                     /translation="MNEKILSPEEARAVRERLRVEGRRLVFTNGCFDILHVGHVRYLE
                     AARALGDALLVAINSDASVRALKGESRPVMSEAERAEMLAALGAVSYVTIFDANSPRT
                     LIQELLPDVLVKGGDYQPDEIHGREEVEAAGGRVLALPFIEGVSTTDIIERIRKTVTS
                     DE"
BASE COUNT         1122 a         2247 c         1891 g         1251 t
ORIGIN      
        1 gacggttctt aggttccgac acgaacgtct taattaacga tcttccattc gacgcacagc
       61 ttgagcatgg acacgtaacc ggagaaacgc gtgcccggct catcgaacga cactttcaag
      121 atggcggcgt ttccgtcgat gtcaaaggct ctcgatcttt cgtctgcgcc gcgcttcgtc
      181 gggcgcgtgt ttgccgctcg cccatttttg cttcgcctgc ggcggcgaat gcaacgaacg
      241 gcgacccgcc gctcactcct cctcgcgccg ttcatcccgc acgacttcat agagcgtcgc
      301 cgcttcctgc ttggaagtct gccgcgcggt cggcacggag agcacgcgca ccgtcaaaaa
      361 gttcgcgcgc cgcgcaagcg tgatctcatc acccacccgc accgcacgcg acgaacgcgc
      421 gaccgcaccg ttcaccacga ccgccccggc ctcgcacaac tcctgcgcca ccgtgcgccg
      481 caacaccagc cgactcgcgc gtaaaaattg atccagacgc atgataaagc agtgacgagt
      541 gacaagtgac aggtgacgag ttaaagacag agttgctgct ttaactcgtc acctgtcact
      601 tgtcactcgt cactgtaatt aaggtttctt gttgccgttg tcggacttct tcttgtcgtc
      661 cttcttggcg gcgggggcgg tggcggcgac cgctgcggga gtggctttct ccgcgtcgct
      721 cttgagcagc gggagcactt catcgcggta gtctttcgcg agttggatgt aggcgtcgcg
      781 gcgcaaattg tcgatgtagg ttttgcgttc cttgtccaga cgggtgtagg tgagcgcctc
      841 gcgcaccctg ttttcgtcga aggccggttc gccgcccgcc gtgcgctcgt ccacccgcag
      901 gatgatgatg ccgttatcgc tgtcgagcgg attggtgacg ccgccaaccg tcttcacgcc
      961 cttgatggcg ttcgacacct cttccttcgt gatgtcggac atggcatacg tgccgagttc
     1021 gcccttgttc tccggcgcga tgcgtttgcc gtccacttcg cgttccgact gcaccatcgc
     1081 tatcgccttg aagtctgcgc ccgcgcgcaa ctgcgcgacc aacttgtcgg cgcgctcgcg
     1141 cacttctttc gggtctttcc cgaccgtgct caagtagatt tccgacagct tgaccgtctc
     1201 cggcttgcgg aatttctctt tgttcgtctc gtagtagttt ttaacttccg tttcgctcag
     1261 gctgtagaag actttctggt caacttcgcg ccccagcacc cacgtcttca tgatctccgt
     1321 gcgcatcgtg gcgcgcaccg aagccggatc aatcttgctc tcgcgcatgg cctgttcgag
     1381 ttgggcgagc gtcttgaagc cctctttctt catcacgtcg agcatgcggc ggttgacctc
     1441 ggcttccacc tcttcggtca tgcccagttc cttgcccttc tgcacgagca actgttcgtt
     1501 gatgagcgtg gcgataattt cggggcgtcg cttcgtgatc tcctccgacg cctgctgttc
     1561 ggtgatcccg cgctgacctt tgatcgcctc caccgcctcg gccatctccc gcttcaaagt
     1621 cgagagcgtg atcacttccg agttgacctg cgcgatcact tcgtcaataa cgcgcggcac
     1681 gccctcttcc tgcgccctga gggaagcggc gttcggcgcg agcagagcgg ccaagagcaa
     1741 tgcgcttgcg ccgacgcggc gggcacgctt taaaaatttt aacttcacag atttttactc
     1801 cttacagatg cggcttgcac ggcgcacgaa ggcgcggcca aaagcctctg cgacaaaatt
     1861 gttaatcgaa ttaacgtctc tgatcaacga atccggctcc gcttaagagc gcggaacaca
     1921 gcaggtttca acggtcgggt gaaatttcaa ggtttcaagt ctcaggcgac aaattcaggt
     1981 tcaagaccgg aaaaccccgg ccttgaaagt tgcgacccga aacccggcga ctcattctat
     2041 tccgcgcggc tgattcgcag caacaggccg tgcgcggttt cgattacgtc ctccgtctca
     2101 tcctcgctca aatcgacgcg caacacgccg ttcggcgtaa aggtggccgc cgtgcgttcg
     2161 gccacgagcg cgagtaattt ttcgggcgcg acgcgcgcct tctcgctcaa cttgatcgcc
     2221 acgcccgtcg gcgtccggtc aagggagagc acgccgacgg cctctgccgt gcggcgcagg
     2281 cgcgcgtagg cgagcaggcg ttcgacggag gcggggatgc gcccgtagcg gtcttccgtc
     2341 tcggcgcgaa tctggcgcag cgtctcgtcg tcgcgggcgg aggcgatgcg cttgtaggtg
     2401 cgcaggcgtt gtcccatgtc cgggatgtag tcttcggaga tcgccacgtc aacgcccaga
     2461 ttgagcgaca cggaagtctc gtcctgcacg tcctcgccgc gcagttccga cacggtgcgt
     2521 tcgagcatct gcgtgtaaag atcgaagccg agcgcgtcca tgtggccgga ctgctggccg
     2581 ccgagcagat tccccgcgcc gcgcagttcg aggtcgagcg cggcgacgcg aaagcccgcg
     2641 ccgaggtcgg aaaactcgcg tatggccgcc agcctgcgac gcgcaatcgg cgtcaactcc
     2701 tgctccgacg gaatcaaaag ataggcgtaa gcgcgtcgat tcgaacgccc gacgcgaccg
     2761 cgcagttgat acagttgcgc cagcccgtaa tgatcggcac gatttatgat gatggtgttg
     2821 gcgcgcggga tgtctatgcc gttctcgatg atggtggtgg cgacgagcac gtcgtatttg
     2881 aaggccataa aatccagcat gacggactcc atctccttct cgttcatctg cccgtgcgcc
     2941 acgcagacgc gcgcctgcgg cacgagccgc tggacgagcg cggcaatcgt ctcgatagtc
     3001 tccacgcggt tgtgtatgaa gaagacctgc ccgccgcgcc cgagttccaa ctctatcccc
     3061 gacttgatga ccgactccga aaactgcacc acctgcgtct gaatcgccag acggtcgcgc
     3121 ggcggcgtct cgatgacgga catgtcgcgc aagcccatca gagacatgtt gagagtgcgc
     3181 ggaatcgggg tggccgagag cgtcagcacg tcaaccttct tcttcaactg cttcagacgc
     3241 tccttgtgcg cgacgccgaa acgctgctcc tcgtcaacca cgacgaggcc gagatcacga
     3301 aacgccaaat ccttcgacaa aatccggtgc gtgccgatga ggatgtccac ctcgcccgct
     3361 tcgacggcgc gcacaacctg cttctgttcc ttcggcgtgc ggaatctgga gagcagttcg
     3421 atcttcacgg ggaaggccgc gaagcgcgcg ctgaacgtct cgaagtgctg gtaggcgagc
     3481 actgtggtcg gcgtcaagac cgccacctgc ttcgactcca tcacggcctt gaacgcggcg
     3541 cgcatcgcca cttcggtctt gccgtagccc acgtcgccgc acaggagcct gtccatcggc
     3601 gtcgtcgctt ccatgtccgt cttcacatct tcgatagcag tctcctgatc cggcgtcggc
     3661 acgtattcaa agccgtcttc aaactcgcgc tgccacggcg agtcagccgc gtaggcgtaa
     3721 ccgccgacca atttgcgttc ggcgtagagg cgcaaaagct cgtcggccat gtcgcgcatc
     3781 tcgcgcttgg ccttggcctt cgtcttctgc cagcccagcc cgccgaggcg gtcgagcgtc
     3841 ggctcatgcc cctccgcgct cgaataacgc tgcacgagat caagccgctc gacgggcacg
     3901 tagagcttcg cgtcctcggc gtagaagagc agcatgaact cgctcttgcg cccgctcaaa
     3961 tcgagcgtct gcaaaccgcc gaaacgcgcg atgccgtgat cgatgtggac gacgaaatcg
     4021 ccgactttca ggtcgcggaa gtccgacagg aaggccgccg tcttcgacct cttccggcgc
     4081 gttctctgcg cgcccgtccc cgacgccgct cccgcgccct gcccctgcgc gccgggcgcg
     4141 cgatgctcca cgctcgcgcc gtcgtgcgcc tcgtcgaaca ggtcgtgctc gacgtgaacc
     4201 accaagcccg ccgaaggcaa ctcgaagccg cccgacaggc gaccgaccgt gaccaacgcc
     4261 tgcccgtctt cgagcgcgcc gccatgctcg cccgccgcta cgacgcgcgc cgcgacgttg
     4321 tactcggcga gcatctcccc cacgcgctcg gccaagcccg cgctcggcat cgcaaacaac
     4381 acgcgcgcgt ctgcctgcgc ctgcacgcgc cgcacgtccg ccgccagatc ggcgatgcgc
     4441 ccgtgataac gacgcgccgc gcgcgtgttc cactcgacct ccggcgcacg ctcgaccgcc
     4501 gggaagagga acagcggcgc ggcattggcg cgcgtgcgcc cgattgtcac agacgccgct
     4561 tcgacttcgc ccgtgaacct ctcgtccgtc gtcgccgccg cgcgcccgag cgtccgcaac
     4621 tcgacgcgcg gcacgcccgc gacgcccgcg cgcaactcct ccgccgtcaa ataaagctct
     4681 tctgggcgca aggccacctc gtccgcgccc tccgtctccg cgtagcgcgc gttcaaagtc
     4741 tcgtaggtgt tgccgagaaa agtttcaagc gcggcgggtt cgtcggccac gaagatcgtg
     4801 tcgcgctgca agtagtcgaa cgcgctcgcg tgcgtggaat ggacgagcgg aacgagccat
     4861 tcccagcccg cgaacgtctc gccctcttcg gcatggacgg tgcggtcgcg cagagcgcgc
     4921 gcatagcgtt cgtccggcca acgctcgcgc gccagttccg cccacaggcg gaaatcgtct
     4981 gcgcccagag catactcgcg catcggcacg atctcgatct cctgtcgctg cgcgatggac
     5041 aactgcgtct ccgggtcgaa ctcgcgtatg gagtcaaccg tgtcgccgaa aaactccacg
     5101 cgcacgggcg cgtcctgacc gggcgaccag acatcgagga tgccgccgcg cagcgagaac
     5161 tcgccgaccg cgccgatggg gtcttcgcgc acgtaaccgg aggcgagcag cagttcgatc
     5221 aagtcttcgg gcgcgtagtc ggcgtcgcgc cggagcgacg ctcctgtcgc cagtagttgt
     5281 tgcggcgcaa tcgtccgccg cgccagcgca cgcgccgtca acaacacgaa accgccctcg
     5341 ccgcgtgcga gacgccagag cgtgagcgcg cgtcgctcca gcgtctccgg gtgcggcgac
     5401 gcgcccgcgt acgggtcgct ctcggaggcg ggcaaagtca gcaccgacgc acgccgcccc
     5461 gcgccgccct gctcctcgcc gtcccgcctc tgtctcaacg cttcgctcca gaaacagaga
     5521 tcgcgctccc actgttcaat atcgcggctc gcctgcacga ccaccgccag acgctttccc
     5581 gtctcgcgtt gcaacgccgc caagacgagc gcacgcgccg cgcccgaagt cagcccgctg
     5641 accgacacca cgcgcgcgcc gccccgcacc tccgccaaaa gacgcgccaa ctcctcgcct
     5701 tccagcacgc gcctcaacgc gccctccacc ggcgaacttt tttgcaccgt actactcatt
     5761 tttaaatcag tgacaagtga cgagtgacag gtgacgagtt aaagcagaac cactgccttt
     5821 aaacctgtcc tccatctcgt cgcttatccc tcgccacccg tcattgtttt atctatcccg
     5881 tcacccgtca ctcgtcactc gtcactgtct ttctaattct ttcgatgatg tcggtcgtcg
     5941 agacgccttc gatgaagggc agggcgagga cgcgcccgcc cgccgcttcg acctcttcgc
     6001 gcccgtgaat ttcgtcgggc tggtagtcgc cgcctttgac gagcacgtca ggcagcagtt
     6061 cttggatgag cgtgcgcggc gagttcgcgt cgaagatcgt gacgtagctg accgcgccga
     6121 gtgcggcgag catctcggcg cgctccgctt cgctcatcac ggggcgcgac tcgcctttga
     6181 gcgcgcgcac agacgcgtcc gagttgatgg cgacgagcag cgcatcgccg agcgcgcgcg
     6241 ccgcctcaag gtaacgcacg tggccgacgt gcaaaatatc gaagcagccg ttcgtgaaca
     6301 cgagccgccg cccttcgacg cgcagacgct cgcgcaccgc acgcgcctcc tccggcgaaa
     6361 gtatcttctc attcataatt tgcgaagcag tgcaatgcaa agaatgctaa cacgaagcgt
     6421 ctggagtagt cagcagtccg ctgccgtttg ccggtcgttc gctccgaatc ttcggcgact
     6481 cccgcgcccc ccgcacgcgg cggagcaccg g
//
LOCUS       DASWNS010000240        23505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_502918, whole genome shotgun sequence.
ACCESSION   DASWNS010000240 DASWNS010000000
VERSION     DASWNS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 23505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23505
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502918"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..477)
                     /locus_tag="VGB76_08215"
     CDS             complement(46..477)
                     /locus_tag="VGB76_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098925.1"
                     /translation="MPLMFIDEDGLSCSRPSWSDMPVHQPAILYVENNGLLAQLVGDL
                     LDLRGWHAWRAENGASARIFLNSQQHYALLLVDNELRDTTGLEVVKFARTLSHRKLLP
                     IMLFSIEDCEREAKAAGANEFLRKPHDLFQMVDTIRRLIEK"
     gene            687..1001
                     /locus_tag="VGB76_08220"
     CDS             687..1001
                     /locus_tag="VGB76_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0098926.1"
                     /translation="MVGTRLKQERLAGKLLQVRLAFGLSQQDMHKRLDVEAIIPYNRI
                     SDYELGKRDPPLAVLLKYARVANVYVDALIDDELDLPDQLPSPTKHEGITRKPAPRTK
                     KR"
     gene            1501..3255
                     /locus_tag="VGB76_08225"
     CDS             1501..3255
                     /locus_tag="VGB76_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098927.1"
                     /translation="MSLEIKSEGVEGVGEAALRPRDLLVAGVLMAALAGVRLWRLDAS
                     CLWFDEVFSVHAARHGWRALVDFVALDLIHPPLFYLSLKGWIGLVGSEAAWWVRVFAV
                     LTATAACVPVMLLCRELGLRPRVGHVALGLMAASGFLIQHGREVRMYSLLLLLAACSL
                     WLFARYTWGARGRKRVGLGAPVSLFVVNLLLVYTHYYGWLLVGHELIFLAVWGRERLK
                     EFAAMSVALAVCYSPWLWMVWEASGEGEGLEQNIGWAIAPRVAEIVQPFLLLHEPFRF
                     RQNTHERAVLRVNVWLALAVFVPAFVALGWRVVGRRRAGAGKLETTNGEMRDEGRDAD
                     VTFALKFLIFFSTAPVVAAFLLARVLPHSVWGVRHLIIIAPVYLLLAAYALYALRPLW
                     LATTIRLLLGCWLALAAMLWLVRRAEVPPVWCAWETLARRVVETETTAGAGEAATGEG
                     GTGREVKIYATEELVAYHLWYALSPAGSARSADAGRFRVAAIANLPGLSEDRAFFLPR
                     GFAEVSRADAGAEEHGGEGALREEHFWAAFRDTSWNAGHPLLRLLSERGYEIEKRFEL
                     TASGQKAFIVSARRRRER"
     gene            complement(3345..3722)
                     /locus_tag="VGB76_08230"
     CDS             complement(3345..3722)
                     /locus_tag="VGB76_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0098928.1"
                     /translation="MKHSEGFERLAEDAKSRIRELNVEQAREHLEANPQARLIDVRED
                     REWQAGHAAGASHMGRGIIERDIEVAIPDKETELILYCGGGYRSALAADNLQRMGYRN
                     VYSMDGGWTAWKESGAPMTTEGE"
     gene            3888..5291
                     /gene="lpdA"
                     /locus_tag="VGB76_08235"
     CDS             3888..5291
                     /gene="lpdA"
                     /locus_tag="VGB76_08235"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY0098929.1"
                     /translation="MATEQFDVTVIGAGPGGYVAAIRGAQLGLKVALVEKEKRLGGTC
                     GLRGCIPTKALLMSAHNYELAQHLGDHGIQVSEVKLAFDAVQKRKDKIVNKNAAGVSY
                     LMKKNKVTVFNGTGKLALPGKVEVTGADGQRQTIETKNIIIATGSVVRPIPGFETDGE
                     RVVNSDHILELKQVPKSLIVMGAGAVGVEFASVYSRFGTETTIVELMPHLLPLEDEEV
                     SVELEKSFRKRGIKQQLGTRLEKMEKTDAGVVVTGRDAKGAEVRLEAEMFLVAVGRMP
                     YLEGLGLEGTKIKVERGNVQVDEFCRTGEPGVYAIGDVIPTPWLAHLASKEGILVVEQ
                     IAGQKPHPINMRHVPSCTYCDPEVASVGLTEAAAKKEGYDVKVAKFPFSASGKARILG
                     EEEGFIKIVSETKYDELLGVHIIGAHATELIAEAGVALQLESTAEELGRTIFAHPTVS
                     EAMMEAAEGIHGLTIHF"
     gene            5394..6539
                     /locus_tag="VGB76_08240"
     CDS             5394..6539
                     /locus_tag="VGB76_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY0098930.1"
                     /translation="MKTETKNKSASRQGAAKGKKATAAEGNGASTAATTVADEELICT
                     TETTLGRDQLLELYRYLLLTRLVEERLVNLYRQTKVVGGVFRSLGQEATAVGTCYALE
                     PQDFISPLIRDLGAVLTKGIRPREIFAQYMAKAWGPSEGRDLNIHFGDMSKGFIGPIS
                     HLGDMIPVMTGIALGARMQQKNLVAMAYIGDGGMSTGAFHEGLNFAAVQRLPVIIIAE
                     HNWYAYSTPTSKQTAVENLADKAAGYGIPGHIVDGNDVIACYEVTKRAAEYARAGGGA
                     VLIEAKTYRRKGHAEHDDQRYIAPTELEMWEKRDPVDRFTRHLLKQGIATQAELEEIV
                     AGVNREIDEDVAWAEESPMPEAEKAAYGVYDNSIVAPAFRPKIFGGN"
     gene            6561..7109
                     /locus_tag="VGB76_08245"
     CDS             6561..7109
                     /locus_tag="VGB76_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098931.1"
                     /translation="MRRSNLAIAFVLLALLSGAKARGQQVEERVQLKDGRVSFVLPAG
                     FKPMSKEEINFKFGRNGAANAPDFAYSNEGQNVSVAVGFRGSGLQHAPLEELKKGMEA
                     QFERSIPGVEWIEREIITRDGRRWIHLHLKAAAIDTGVINDMYFTIFDGQLLLFNFNS
                     TIAQHDKYKESLRQSAQSINVK"
     gene            7133..8161
                     /locus_tag="VGB76_08250"
     CDS             7133..8161
                     /locus_tag="VGB76_08250"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY0098932.1"
                     /translation="MSTAVKKEHKSNRPARGGVATFVEAIRQGIWEEMERDPSVFVIG
                     EDVGAYGGAFKVTDGLLDEFGEGRVIDTPISEAAIVGAACGAALMGMRPVAEFQFIDF
                     ISPGFDMLTNYAAKCRYRWGAALPTVFRGPCGAGVHSGPFHSVNAESFFLNTPGLKIV
                     EPSTAYDAKGLIKAAIRDPDPVLYFEHKKLYRLPRLREELPEDDYIVEIGRARTAREG
                     RDLSIITFGAQVLNALDAAAELEKEGLDVEVIDLRTLAPLDKEAILQTVKKTNKIMIL
                     HEAARTGGIGGEIAATIAEEAFEWLDAPVVRLAAIDTPVPYSPPLEEYHLPQIKDVLA
                     AARKLAAY"
     gene            8230..8523
                     /locus_tag="VGB76_08255"
     CDS             8230..8523
                     /locus_tag="VGB76_08255"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEY0098933.1"
                     /translation="MPKYVIEREVPDAGKLSGDALQSMSQKSCSVLNELGTQIQWVQS
                     YVTDDKIYCVYIAPNEEMVREHAAQGGFPANRISEIRTIIDPTTAEKTVTSDK"
     gene            8604..10136
                     /locus_tag="VGB76_08260"
     CDS             8604..10136
                     /locus_tag="VGB76_08260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY0098934.1"
                     /translation="MSTEVVMPQMGESIAEGTITRWMKQVGERVERDEPLFEISTDKV
                     DAEIPSPVAGTLIEIKFKEGETVEVNKVVAYIGAEGETAAAAPAGDGAAAATPAAPPP
                     TPATPAPAAQAAATSSAPPPPPSMATGAQASNTAQPAGASAASPQTAEVAGRSYDSER
                     PGAKAEGQSLASGAGNGGAQAVDASTGANGGAQSVEDLRRTKSSPLVRNIAREHNIDI
                     TQLEGTGTSGRVTKNDILSFIESGATAATPQPTTAEARAPQPPPAIQTPPPPPQVQPG
                     VGDRVEPMTLMRKKIAEHMIMSQNTSAHVATVYEIDMTRIAKLRDRHKAEFQQRTGTK
                     LTYMPFIFQAITSALRKFPIFNAQVSGDQIIYKQDINLGMAVALDWGLIVPVIKRADD
                     LSISGLARAANDLADRARNKQLKPDEVAGGTFTITNPGVFGGLFGTPIINQPQVAILG
                     IGTIEKRAKVLTTPEGDDYIAVRHMAYFALSFDHRIIDGADAERFLAYVKGLLEAGQF
                     QV"
     gene            10405..13803
                     /locus_tag="VGB76_08265"
     CDS             10405..13803
                     /locus_tag="VGB76_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HEY0098935.1"
                     /translation="MKSLARFAGDRSARPLPLATDLPFSRLFTRTIRRASIVFMISLA
                     GLCSNFLIPALAQDEDVTPPSVEINQAEEQADPTNSEPVQFTVIFSEPVFGFETGDVT
                     LGGTAGATTAEVFDSGDQMTYTVLVSGMTMNGTVVASIAAGVATDAAGNRNTASISRD
                     NTVTFNGFVVPDTFTVTKTADTNDNLCNADCSLREAINAANANPGADTILFDIPASDP
                     RRNATTGVYTISVSALLGALPQIVEQVTIDGYTQGTISTPSDTSDDARPNTNPLSAGL
                     NMRLLVELTDDASGIANANGLDVGEAGFNSRIRGLAIYGFSTGAGVNITGAEGVRVEG
                     NFLGVRADGSTGVSNNYGVRITGAPRASIGGTNAGASNLISGNTQAGVLITGVIAAGE
                     DRNLVQNNLIGTNALGLAGLPNAEGASGINKGGVLIIEASGVLVGGAGVGNVISGNNG
                     IGVIISGSIAEGGAPATDNAVRGNLIGVAADGATALANSGDGIEITNGQNNFIGGTAS
                     GQGNTVSSNTANGIALIGSLFVGGESSNNIIAGNTIASNGMDGVLVSAGTGNRINSNS
                     IYSNGQQGIDLLGDGVTPNDPNDADPLAGLNANNLQNFPVVSFAVRDTETGVTTITGT
                     LDGGTQGVVYFIELYANPACDGTNGEGRLPLGGVLTGAADANGDVSFTFQTSADLSGQ
                     VITATATDETTNDTSEFSACTAVTTTSAPPQGVPTTGQIIISEFRLRGTDPDGGGAET
                     GALDEFIEIYNNTDSDIVVVDSSPLPPLTSNGWAIVSSDAPTTAKFVIPSGTRIPARG
                     HYLVANAVGYSLGLYPSGNDGSVQTTAIEDGGYNVDIPDNAGIALFRTANPLFFLNPV
                     ERLDAAGFAGGGVLFTEGTPLTPTGGVTASLQHSFVRRMTTGLPQDTNDNEADFEFVE
                     VTGATSGGRRARLGAPAPENLTSPIQRNALIKAMYVDPQCTFGTPESACARVRTAQGA
                     NPTDAAFGTLLLRRRFRNATNQAVTRLRFRLVDITAGTQESGGADLRALSGSNVSVTN
                     SRGETLELKGLTLEELSAPSPSQPVGGGLNSSLSAGTVTLAQPLAAGNFIDVEFRIGV
                     MRNGSFRFLVNVEALFAEANPETEFGGSNRKSKGAKSNVRKQAR"
     gene            complement(14040..15371)
                     /locus_tag="VGB76_08270"
     CDS             complement(14040..15371)
                     /locus_tag="VGB76_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098936.1"
                     /translation="MKRLLGRRAPAVSERESLIGKNDAAKRSPSSVYLQLIEDEETGR
                     EFGRRPLGVRLGAVFATLAVGLSAGAFSAFASDDDSFVTPSKDGSADDDNDIDDTATG
                     TGTGVNTATATSTDLGTNDATGTNTGTGAGTATGTGTRNGRTATGTATGTGALTATNT
                     ATATNLNTRDATATQTGTGTGVNTATATATQTAAGNIGRGKSGKNKSGKGKGGDNNND
                     TATGTATATGINTATNTGTATNPLTGDATATRTATATGNNTLTGTATRTATGTRLTNT
                     DGTATATATATGVNTATNTATATDPATQDRTATATGTGTGVATGTATNTATVTGTRTV
                     TGRTATATATGTGAVTVTNTATGTNPQTVDGTATNTATGTGAATATNTATATGTVGGG
                     TATGTATATGDTTATNTATATNPSTQDTTTGTGTGTGADTGTNTKTKTKNN"
     gene            complement(15460..16506)
                     /locus_tag="VGB76_08275"
     CDS             complement(15460..16506)
                     /locus_tag="VGB76_08275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0098937.1"
                     /translation="MFSSPVSRSSSWLKDRWHAVRPAQPGGRQSDEEVAPAAEESSQH
                     GSEVSKRREEEKASWNFEAGAQIAEGRTVLKRLSGGNRYEVFLTWDERLFAITVTKVL
                     RPDQAEDEHARGELQREVEVLRRLAHPCLVRCFDAVLDSPHPHVMLEHLEGPTLRRLI
                     KQQTTIPLDQLLPLALHVAAVLQYMANEEFVHLDVKPSNIVMGVPPRLIDLSIAQSFE
                     SAAQLRHSIGTDPYMPPEQCDPAARPGSVGHAADVWGLGATLYHATTGETPFPRGEAK
                     SRDRTLRFPQLVSEPKPLPKHVPQPLQELISSMLAKDPAERPAASEVATSLEPLVALI
                     SPRLKATRRGIHVR"
     gene            17138..18184
                     /locus_tag="VGB76_08280"
     CDS             17138..18184
                     /locus_tag="VGB76_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0098938.1"
                     /translation="MSTIALTIVLALASAAFAQDATGSNQTTGSANSRSVASGQKMTV
                     KGVVTSRDIDADTFVVQDSNGVSTTVRLTDRTSVKSKGGFFGGGSTYGPSNILRGLNL
                     EVEGRGDASGNLVAEKVRFGSTDLRVAQSIQARVDPVESRVGTAENRIGQVEQNSQRL
                     SGQLDELAAVSNAARGGAKAAQESADAAIAGVNVTNERISALDDYVAQQTVAVNFGVG
                     SAVLTPASKTTLDQIAQQALKARGYVLEVRGFASAEGSEEKNRMLSQRRADAVVRYLA
                     YTHNIPLRRIVTPFGYGEQQAVADNTTRTGRQQNRRVEVSVLVNRGLMQTAPVMSTAK
                     PDAATTPDQQPDQQ"
     gene            18320..19687
                     /locus_tag="VGB76_08285"
     CDS             18320..19687
                     /locus_tag="VGB76_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0098939.1"
                     /translation="MNSTQNRLRALLLLLSIFPGGTLPLAAAPRFESKAETSAGVQTA
                     RSSSRRRPAPAKTTPKQQTPATQPVRVPQLAPRTTQATQATQTPQATPVPVPTQTPTP
                     QPRATRPPVADFGTPPQKTKPDNDKVQDQVTDDAGAISDDDEIVRVTSNLVVVPVSVT
                     NERGEPVQGLKATDFRLEEEGRAQEIAHVSDADQVPLDIAILFDVSSSVGQRFAFEQE
                     AAARFLKQVLKPIDRAAVFRIEETPRLEQQLASAEIASATLLKIPAPQRPTPTAFYDS
                     TISAARYLAANAPGRHRRVIVVISDGDDNYSARVKEGEVEEARAVNRGEKLPATALTR
                     QRETHRKALLEVQREVQRADAVFYSINPSGGGLQFNARGARAQEGMQQLAETTGGTSF
                     KPEQLEELDDIFRRIAAELRAQYLLQYYSTSDAPNGKFLNIKVRTPTRADARVRARSG
                     YYVKK"
     gene            complement(19777..20304)
                     /locus_tag="VGB76_08290"
     CDS             complement(19777..20304)
                     /locus_tag="VGB76_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098940.1"
                     /translation="MRFVYSLTLTGLLLLLTPLENFAQPRQRRASAPKRPTATRGRRP
                     ASTAANALALQDGRQRVGEKIKQISNFVYLYGRFSKDFEAAVGQGGQPDATASALKNR
                     LISSLQDIREGLDGLELHFRTTPELERFSIRLSGVALGAANAEQQASSNRLDAAGRSL
                     LEVINRLTDVLQEMH"
     gene            20483..21151
                     /gene="lipB"
                     /locus_tag="VGB76_08295"
     CDS             20483..21151
                     /gene="lipB"
                     /locus_tag="VGB76_08295"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY0098941.1"
                     /translation="MPKRTLQVRRLGRMEYGAAYKIQKEIEAARKTGAGADTLLLVEH
                     PHVLTLGRRADESAVLAAPETLASRGVALFETNRGGKATYHGLGQLVGYPIINLSPER
                     EDVHRYVRELEEALIAALADFRIESFRIKGLTGVHTARGKVAAIGVHISRWVTTHGFA
                     LNVNTDLSFFDLIIACEGEPVTSMYELLGHELPLREVEDRIVARFAEVFEMTVTPEDE
                     YANA"
     gene            21138..22094
                     /locus_tag="VGB76_08300"
     CDS             21138..22094
                     /locus_tag="VGB76_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY0098942.1"
                     /translation="MLTLDFIREAHARIRSRVHRTPVISSRAFNEAAGREVFFKCENF
                     QRAGAFKMRGATNKILTLDAEERARGIVAFSSGNHAQAVALAARDAGARAVIVMPQDA
                     PRAKVAATRGYGAEVIFYDRQRDDREAVTRDVAEREGLVLVPPYDDYYVMAGQGTCAL
                     ELLEETAELDALVVPCGGGGLFAGASVAAKGIRREILCFAVEPETGDDTRQSFRQGER
                     VSIPPPPTIADGARLESPGALTFPFVQQHAEDVLTVSDAELVEALKFLLFRLKILVEP
                     TGALGVAAVLFNKLPANIRRIGVVISGGNIDADALARLVQEE"
     gene            22187..22342
                     /locus_tag="VGB76_08305"
     CDS             22187..22342
                     /locus_tag="VGB76_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098943.1"
                     /translation="MDKNNSLKEPLQVEKNAPVRNVVRFWFIALALIIIGLLIIGLIF
                     GNFGIQR"
     gene            22416..23381
                     /gene="lipA"
                     /locus_tag="VGB76_08310"
     CDS             22416..23381
                     /gene="lipA"
                     /locus_tag="VGB76_08310"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY0098944.1"
                     /translation="MSRELKVISNNPPPPQGRPTGRKPDWLKIRIGDPTNQNHVFNLI
                     EGLNLHTVCQEARCPNIFECWSDRTATFMLGGDICTRHCGFCAVGKGQPGALDPDEPR
                     HVAEAVRHLDLAYAVITSVNRDDLPDGGAGHWAQTIREVRAANPACKIEVLIPDFNGD
                     EAALDTVLDAQPDVLNHNTETIARLYRRVRPDAKYAQTLTLLERAARRRDAEERGMLT
                     KSGIMVGLGETFEEVVELMRDLRAVSCDIMTIGQYLQPHARRLPVERYVTPEEFARWR
                     DVGMSMGFHHVESSPLTRSSYHARQQVEGTAQEETKRDEEAVAVS"
BASE COUNT         4513 a         7177 c         7454 g         4361 t
ORIGIN      
        1 ctcatcactt ttcgatactc attactcatc acttttcgat cctcatcact tttcgatcaa
       61 cctgcggata gtgtccacca tttggaagag gtcgtgcggc ttgcgcagaa actcgttcgc
      121 gcccgccgct ttcgcttccc gttcgcagtc ctctatcgag aacaacatga tcggcagcaa
      181 cttccggtgc gacagagtgc gcgcgaactt caccacctca agccccgtcg tatcacgcaa
      241 ctcattatcc acgaggagca gtgcatagtg ttgttggctg ttcagaaaga tgcgtgcgct
      301 cgcgccgttc tccgcgcgcc aggcgtgcca gccgcgcagg tcgagcaggt cgccgacgag
      361 ttgggcgagc agcccgttgt tctcgacgta gaggatcgca ggctggtgca cgggcatgtc
      421 gctccacgat gggcgcgagc atgaaagtcc atcttcatct atgaacatga gaggcatttt
      481 gttaagatgc gcgcgttcaa atgttgaaaa tccgcgacgg tcgtatgcgt agctcacctc
      541 aacctcaagc gagtgatgag catcgccgtt gagtctggca ggccaactct ctcggcgaac
      601 aagttacgca gacaaagttt ataccatata tggtatccat gcaagagtga ttttgctggc
      661 tctgcactgt caggaggcga ctcctgatgg tggggacacg cttaaagcaa gagcgacttg
      721 ccgggaagtt gttacaggtt cggctggcgt tcggactttc tcaacaggac atgcacaagc
      781 gtttggatgt cgaagctatt atcccttaca accgcatttc ggattacgaa ttaggcaaac
      841 gcgatccgcc gctcgcagtc cttttgaaat atgctcgcgt cgccaatgtc tatgtagatg
      901 cgttgattga cgatgaatta gatttacctg accaactacc cagccctacc aaacacgaag
      961 gcatcacacg caaacccgcc ccgcgcacga agaagcgttg acacttaaaa cacttagagg
     1021 gtcgcccccc ggcgaccctc taagtactcg cacactaccc ggcaataacc ccgcgcggcg
     1081 cgtctccctt aacccatcaa ctgcacgccc cacacgcccg cgcgccagtc acaatcaacc
     1141 ttctatactt tcgcctccgc ctccggcttc cggcgtgaca taaccgctca acgaaatatc
     1201 accacagacc cccgtaataa aatttccccg cgcccgaacc gcatccccac aaccctcaaa
     1261 tgcggactca tattactttt ccatgtgccg caccttcgga gtgcggcagt gcgccgaact
     1321 cttttcaagc cttcgaacac tgcccgtcga atagaccttg cgataactga tgcgctgaac
     1381 accggcgacg agtgcggagc gatggacgta ccggcgtcaa cccgaagccc ggtttcagac
     1441 aaggttcgag aatcaaggct cgacgccttg tgcgttgggg cattatgatg cgtgccgccg
     1501 atgtcattgg agatcaagag cgagggagtg gagggcgtgg gggaggctgc gctgaggccg
     1561 cgcgatctgt tggtcgcggg cgtgttgatg gcggcgttgg cgggggtgcg cctgtggcgt
     1621 ctggatgcgt cgtgcctgtg gtttgacgag gtgtttagtg tgcatgcggc gcggcacggg
     1681 tggcgggcgt tggtggattt tgtggcgttg gatttgattc acccgccgct gttttacctg
     1741 tcgctcaaag gttggatcgg gttggtgggg agcgaggcgg cgtggtgggt gcgtgtgttt
     1801 gcggtgttga cggcgacggc ggcgtgcgtg cctgtgatgt tgctgtgtcg tgagttgggc
     1861 ttgcgcccgc gcgtggggca cgtcgcgctc gggctgatgg cggcgagcgg gttcctgatt
     1921 cagcacgggc gggaagtgcg tatgtatagt ttgttgttgc tgctcgccgc gtgctcgctg
     1981 tggctcttcg ctcgttacac atggggcgcg cgggggagga agcgggtcgg cctcggcgcg
     2041 ccggtgtctt tgtttgtggt gaacctgttg ctggtctata cgcattatta cggttggctg
     2101 ctggtggggc atgagttgat ctttttggcg gtgtgggggc gggagaggtt gaaagagttc
     2161 gcggcgatgt cggtcgcgct ggccgtgtgc tatagcccct ggttgtggat ggtgtgggaa
     2221 gcgtcggggg agggggaggg gctggagcag aacatcggct gggcgattgc gccgcgcgtg
     2281 gcggagatcg ttcagccgtt tctactgctg cacgagccgt ttcgctttcg acagaacacg
     2341 catgagcgtg cggtgctgcg cgtgaacgtg tggctcgcgc tggctgtgtt cgtcccggcg
     2401 ttcgttgcgc tcggctggcg tgtcgtcggg cggcgacgtg cgggggcggg aaagttagag
     2461 acgacgaacg gagagatgcg cgatgaggga cgtgacgcgg acgtgacgtt cgccttaaag
     2521 ttcctgatct ttttttcgac ggctccggtc gtcgccgctt tcctgctggc gcgcgtgttg
     2581 ccgcactccg tctggggcgt acgccacttg atcatcatcg cgcccgtcta tctattgctc
     2641 gcggcctacg cgctctacgc gctgcgtccg ttgtggctcg cgacgacgat cagactgctc
     2701 ctcggttgct ggctcgcgct cgcggcgatg ctctggctcg tgcgacgcgc cgaggtgccg
     2761 cccgtatggt gtgcgtggga gacgctcgcg cgccgggttg tcgagacgga gacgacggct
     2821 ggcgcgggcg aggctgcgac gggcgagggt ggcacggggc gcgaggttaa gatttatgcg
     2881 acggaagaac tcgtcgctta tcacctgtgg tacgcgctca gtccggcggg gtctgcgcgt
     2941 tcggcggacg ccggtaggtt ccgcgtcgcc gctatcgcaa atctacccgg cctctcggaa
     3001 gacagggcgt ttttcctgcc gcgcgggttc gccgaagtgt cgcgtgcgga cgcgggcgca
     3061 gaagagcacg gcggcgaagg agccttgcgc gaagaacatt tctgggcggc ctttcgcgac
     3121 acttcctgga acgcagggca cccgctcttg cgcctgctgt cggaacgcgg ctacgagatc
     3181 gagaagcgtt tcgagttgac ggcttcgggg cagaaggctt tcatcgtctc cgcgcgaaga
     3241 aggcgcgagc gttgagcaag cctgccaggc gacagccgcc cacagggctt cgagacagaa
     3301 acccattcag gctgatctcg aattcggatt aaacttcatt catttcattc gccttccgtg
     3361 gtcatcggcg cgcccgactc tttccatgcc gtccagccgc cgtccatcga atacacgttg
     3421 cggtagccca tgcgctggag attgtctgcg gcgagcgcgg agcggtagcc gccgccgcag
     3481 tagagtatga gttccgtctc tttgtcgggg atagccactt cgatgtcgcg ttcgatgatg
     3541 ccgcgcccca tgtgggaggc tccggcggcg tgcccggcct gccattcgcg gtcttcgcgc
     3601 acgtctatga ggcgcgcctg cgggttcgcc tccaagtgct cgcgcgcctg ttcgacgttg
     3661 agttcgcgga tgcgcgattt ggcgtcttct gctaatcgtt cgaatccttc ggagtgtttc
     3721 atcagaggtg tcggttctcc cgtcgctgga tgaaaaagac gggaggcatt ataatccctg
     3781 tttgcaacag caacccataa aatcaagtat ctatttcgga gcgtcggcgc gcgagcggtc
     3841 tgcgtgaacg ggggtcgtgc cgtctccact tttgagaggg aagagtcttg gcgacagaac
     3901 aatttgatgt gacggttatc ggcgcaggcc ccggcggcta cgtggccgcg atcaggggtg
     3961 cgcaactcgg tctgaaagtt gcgctcgtgg agaaggagaa acgcctcggc ggcacctgcg
     4021 gcctgcgcgg ctgcatcccg acgaaggcgc ttctgatgtc ggcgcacaac tacgaactcg
     4081 cgcagcacct cggcgaccac ggcatccagg tttccgaagt caaactcgct ttcgacgccg
     4141 tgcagaagcg caaggacaag atcgttaata agaacgcggc gggcgtcagc tacctgatga
     4201 agaagaacaa ggtgacggtc ttcaacggca cgggcaaact tgccttgccg ggcaaggtcg
     4261 aagtgacggg cgcggacggc cagcggcaga ccatcgagac gaagaacatc atcatcgcca
     4321 cggggtcggt cgtgcgcccg attccgggct tcgagacgga cggcgagcgc gttgttaaca
     4381 gcgatcacat tctggaactc aaacaggtgc cgaagtccct gatcgtgatg ggcgcgggcg
     4441 cggtcggcgt cgagtttgcc agcgtctatt cgcgcttcgg gacggagacg accatcgtgg
     4501 agttgatgcc gcacctgctg ccactcgaag acgaagaggt gtcggtcgaa ctcgaaaaat
     4561 cgtttcgcaa gcgcggcatc aagcagcaac tcggcacgcg cctggagaag atggagaaga
     4621 cggatgcggg cgtcgtcgtg acgggcagag acgcgaaggg cgcggaagta aggctcgaag
     4681 cggagatgtt tctcgtcgcc gtcgggcgca tgccgtatct cgaaggcttg ggactcgaag
     4741 gcacgaagat caaggtcgaa cgcgggaacg tgcaggtgga cgagttctgc cgcacgggcg
     4801 aacccggcgt gtacgctatc ggcgacgtga tcccgacgcc gtggctcgcg cacctcgcgt
     4861 cgaaagaggg catcctcgtc gtcgagcaga tcgctggaca gaagccgcac ccgatcaaca
     4921 tgcgccacgt cccttcgtgc acctactgcg acccggaagt cgcctcggtc ggtttgacgg
     4981 aagcggcggc gaagaaggaa ggctacgacg tgaaagtcgc taagttcccg ttctccgcct
     5041 cgggcaaggc acgcatcctc ggcgaggaag agggcttcat caagatcgtc agcgagacga
     5101 agtatgacga actcctcggc gtccacatca tcggggcgca cgcgacggaa ctgatcgccg
     5161 aagcgggcgt cgcgctccaa ctcgaatcca cggcggaaga actcgggcgc accatcttcg
     5221 cgcacccgac cgtctcggaa gccatgatgg aagccgccga aggcatccac ggattgacga
     5281 ttcattttta aagacagtga cgagtgacga gtaacgggtg acaagttaaa acatcaagaa
     5341 cttgtcttta acttgtcacc cgtcactcgt cacttgtcac ttgcgaagca actatgaaga
     5401 cagagacgaa gaataaatct gcgagcagac agggcgcggc taaaggtaag aaggctacgg
     5461 cggcagaggg caacggcgcg agcacggcgg cgacgaccgt ggcggacgaa gagttgatct
     5521 gcacgacgga gacgacgctt gggcgcgatc agttgcttga gttgtatcgc tatctgctgt
     5581 tgacgcgcct cgtcgaagaa cgtctggtga atctgtaccg gcagacgaaa gtggtcggcg
     5641 gcgtctttcg ttcgctcgga caggaagcga ccgccgttgg cacctgctat gccttagagc
     5701 cgcaggattt catctcgccg ctcatccgcg acctcggcgc ggtgctcacc aaaggcatcc
     5761 ggccgcgcga gattttcgcg cagtacatgg cgaaggcgtg ggggccgtcc gaaggccgcg
     5821 atctcaacat ccacttcggc gacatgtcga aaggcttcat cggcccgatc tcgcacctcg
     5881 gcgacatgat ccccgtgatg acgggcatcg ccctcggcgc gcggatgcag cagaagaacc
     5941 tcgtggcgat ggcctacatc ggcgacggcg gcatgagcac gggcgcgttc cacgagggct
     6001 tgaacttcgc cgccgtgcag cgtctccccg tcatcatcat cgccgagcac aactggtatg
     6061 cctactcgac gcccacttcc aaacagaccg ccgtggagaa cctcgcagac aaggccgcgg
     6121 gctacggcat ccccggccac atcgtagacg gcaacgacgt tatcgcctgt tatgaagtga
     6181 cgaaacgcgc ggcggagtat gcgcgtgcgg gcggcggcgc ggtgctcatc gaagccaaga
     6241 cctaccggcg caaaggacac gcggagcacg acgaccaacg ctacatcgcg cccacggagc
     6301 ttgagatgtg ggagaagcgc gaccccgtgg atcgcttcac gcggcatctc ctcaaacagg
     6361 gcatcgcgac gcaggccgaa cttgaagaga tcgtcgcggg cgtgaaccgg gagattgacg
     6421 aagacgtggc gtgggcggaa gagtcgccga tgccggaggc ggagaaggcc gcttacggcg
     6481 tctacgataa ctcgattgtc gcgcccgcgt ttcgcccgaa gattttcggg ggcaactgaa
     6541 ttttcggaga caagtgaaat atgagacgtt caaacctcgc catcgctttt gtcttgctgg
     6601 ctctcctgtc gggggcgaag gctcgcgggc agcaagtcga ggaacgcgtg caactcaagg
     6661 atgggcgcgt gagctttgtg ctgcccgccg ggttcaagcc gatgtcgaaa gaggagatca
     6721 atttcaagtt cgggcgcaac ggcgcggcta acgccccgga cttcgcctac agtaacgagg
     6781 ggcagaacgt ctcggtggcg gtgggctttc gcggcagcgg acttcagcat gcgcctttgg
     6841 aagagttgaa gaagggcatg gaagcgcaat tcgagcgcag cattcccggc gtcgaatgga
     6901 tcgagcgcga gatcatcacg cgcgacggca ggcgttggat tcacctgcac ttgaaagcgg
     6961 cggcgattga caccggcgtc atcaacgata tgtacttcac gatcttcgac ggccaactgc
     7021 tcctgttcaa ctttaattcc accattgcgc agcacgacaa atacaaagag agcctgcgcc
     7081 agagcgcgca gagcatcaac gtcaaataag aggcagcagc tttctcaaca acatgtcaac
     7141 cgcagttaaa aaagagcata aatcgaaccg cccggcgcgc ggcggcgtgg caacgtttgt
     7201 cgaagcgatc cggcagggca tctgggaaga gatggaacgc gatccgtccg tcttcgtgat
     7261 cggcgaggac gtgggcgcat acggcggcgc gttcaaagtc acggacgggc tgctcgacga
     7321 gttcggcgag gggcgcgtga tcgatacgcc catctcggag gcggcgattg tgggcgcggc
     7381 ctgcggcgcg gccctgatgg gcatgcgacc cgtcgccgag tttcaattca tagacttcat
     7441 ctcgccgggc ttcgacatgt tgacgaacta cgcggcgaag tgtcgttatc gttggggcgc
     7501 agcgttgccg accgtgtttc gggggccgtg cggtgcgggc gtccattcgg gtccgtttca
     7561 ttcggtcaac gccgaatcgt tcttcctgaa cacgcccggc ttgaagatcg tcgagccttc
     7621 gacggcctac gatgcgaagg gcttgatcaa ggccgccata cgcgatcccg atcccgtgct
     7681 ctacttcgag cacaagaaac tctatcgcct gccgcgcctg cgcgaagagt tgccggaaga
     7741 cgactacatc gtcgagatcg ggcgtgcgcg cacggcgcgc gaggggcgcg acctctccat
     7801 catcacgttc ggcgcgcagg tcttgaacgc gctcgacgcg gcggcggagt tggaaaagga
     7861 agggctggat gtcgaggtga tagatttgcg cacgctcgcg ccgctcgaca aggaagcgat
     7921 cctacagacg gtgaagaaga cgaacaagat tatgatcctg cacgaagcgg cgcgcacggg
     7981 cggcatcggc ggcgagatcg cggcgacgat tgccgaagag gctttcgaat ggctggacgc
     8041 gcccgtcgtg cgcctcgcgg cgattgatac gcccgtgccc tattcgccgc cgctcgaaga
     8101 atatcacctg ccgcagatca aagacgtgtt ggcagcggcg cgcaaactgg cagcgtattg
     8161 agaagctgtt agcttttcgt gtctggctga cagctaatct ttcataactc agcgaaagga
     8221 acgacggtca tgccgaagta tgttatcgag cgggaagtgc cggacgcggg gaagttgtcc
     8281 ggcgatgcgt tacagtccat gtcacagaaa tcttgcagcg tcttgaacga actgggaacg
     8341 cagattcagt gggtgcagag ttacgtgacg gacgacaaaa tctattgcgt ctatatcgcg
     8401 ccgaacgagg agatggtgcg cgagcacgcg gcgcagggcg ggtttccggc caaccgcatt
     8461 tccgagatca ggacgatcat tgatccgacg acggctgaga aaacagtgac aagtgacaag
     8521 tgacgagtga cgagaggaaa gacaaattta agacaaattc ttctgtttta acttgtcact
     8581 cgtcactgtc ttagggagaa catatgagta cagaagttgt gatgccgcag atgggcgagt
     8641 ccatcgccga gggtacgatc acgcgttgga tgaagcaggt gggcgagcgc gtcgagcgcg
     8701 acgagcctct gtttgagatt tcgaccgaca aggttgacgc ggagattcca agccccgtcg
     8761 ccgggacgct catcgagatc aagttcaaag agggcgagac ggtcgaagtg aacaaggtcg
     8821 tcgcatacat cggcgcggag ggcgagacag ccgccgctgc tcctgcgggc gacggcgcgg
     8881 ctgctgctac tcctgctgcg cctcctccaa ctccggcaac tcccgcgcct gctgcgcaag
     8941 ccgccgccac gtcgtccgca cctccgccgc ctccatctat ggcgacgggc gcgcaagcgt
     9001 cgaacacggc gcagcccgcc ggagcctctg cggcgtcgcc tcagaccgcc gaagtcgcgg
     9061 gcagaagtta cgactcggag cgtccggggg cgaaggccga aggccagtcg ctcgcgtcgg
     9121 gcgcgggcaa cggcggcgca caggcggttg acgcctcgac gggcgcaaac ggcggcgcac
     9181 agtcggtcga agatttgcgt cgcacgaagt ccagcccgct cgtgcgcaac atcgcacgcg
     9241 agcacaacat agacatcacc caactcgaag gcaccggaac gagcgggcgc gtcacgaaaa
     9301 acgacatcct cagtttcatc gaatcgggcg caaccgccgc gacgccgcag ccgacgacgg
     9361 cggaggcgcg tgcgccgcaa ccgccacccg cgattcagac gccgcccccg ccgccgcaag
     9421 tgcagccggg cgtgggcgac cgcgtggagc cgatgacgct catgcgcaag aagatcgccg
     9481 agcacatgat catgtcgcag aacacgtcgg cgcacgtcgc caccgtctat gagatcgaca
     9541 tgacgcgcat cgccaaactc cgcgacaggc acaaggcgga gttccagcag cgcaccggca
     9601 cgaagctcac ctacatgccg ttcatctttc aggccatcac aagcgcgctg cgcaaattcc
     9661 cgatcttcaa cgcgcaggtt tccggcgacc agatcatcta caagcaggac atcaatctcg
     9721 gcatggccgt cgcgctcgac tggggcttga tcgtacccgt catcaagcgc gcagacgatc
     9781 tctccatctc cggcctcgcg cgcgccgcaa acgatctggc cgaccgcgcg cgcaacaaac
     9841 aactcaagcc cgacgaagtg gcgggcggca cgttcaccat caccaatccg ggcgtcttcg
     9901 gcggactctt cggcacgccg atcatcaacc agccgcaggt cgccatcctc ggcatcggca
     9961 cgattgagaa gcgcgccaaa gttctgacca cgccggaagg cgacgactac atcgccgtcc
    10021 gccacatggc ctacttcgcg ctctccttcg accatcgcat catcgacggc gcggacgccg
    10081 aacgcttcct cgcctacgtc aaagggttgt tggaagcggg acagtttcag gtatagcccg
    10141 gcggcgaaaa cgcagcgacg gctgcaacgc cgcgcatgtc tggcacaagc gggaaagggt
    10201 gacgcgtaac cgtcaccctt tcccttgaga gtgcatgtgt catgcaatag ttcgttgcgc
    10261 cgctgtctac ggcagtattc tcgccccgac ccgctgaact attaaatccg cttcctctcc
    10321 cgtcgcctga gagatgtgag ggagcaccgg tcttcaagct ctttaccaaa cgcaaaccca
    10381 cagggtagaa agatattctt ccagatgaaa tcacttgcgc gcttcgccgg agacaggtct
    10441 gcccgtccgc tcccgctcgc tacagacctg cccttctcgc gtctgttcac acgcacgatt
    10501 cgtcgcgcgt ccatcgtgtt tatgatctcg ttagccggcc tctgctctaa cttcttgatt
    10561 cccgcactgg cgcaggatga agatgtcacc ccgccatcgg tcgagatcaa tcaagccgaa
    10621 gaacaggccg acccgacgaa cagcgagccg gttcagttta cggtcatctt ctccgagccg
    10681 gtcttcggct tcgagacggg cgacgtgacg ctcggcggca cggcgggcgc aaccacggcg
    10741 gaagttttcg acagcggcga tcagatgact tacacagtgc tcgtctcagg catgaccatg
    10801 aacggcacag tggtggcgag catcgcggcg ggcgtggcga cggatgcggc gggcaaccgc
    10861 aacaccgctt caatctccag agacaacacc gtcaccttca acggcttcgt cgtccccgac
    10921 acgttcaccg tgaccaagac tgcggacact aacgacaacc tgtgtaacgc ggattgctct
    10981 ctgcgcgagg ccattaacgc ggccaacgcc aaccccggcg cagacaccat cctcttcgac
    11041 atccccgcct ccgacccccg ccgcaacgcg acgacgggcg tgtacactat cagcgtcagt
    11101 gccctgctcg gcgcgctgcc gcagatcgtc gaacaagtga cgattgacgg ctacacgcag
    11161 ggcaccatct ccaccccgtc cgatacgtcc gacgatgcgc gtccgaatac gaacccgctc
    11221 tccgccggac tcaacatgcg cctgctcgtc gaattgaccg acgacgcgag cggcatcgcc
    11281 aacgccaacg ggcttgacgt gggagaggcc ggtttcaaca gtcgtatacg cggcctcgcc
    11341 atctatggct tctcgaccgg cgcgggcgtc aacataacgg gcgcagaagg cgtgcgcgtc
    11401 gagggcaact tcctcggcgt gcgcgccgac ggcagcaccg gcgtctccaa taattacggc
    11461 gtccgcatta cgggcgcgcc ccgcgcaagc atcggcggga caaacgccgg ggcgagcaat
    11521 ctcatctccg gaaacacgca ggcgggcgtg ctcatcacgg gcgtgatagc ggcgggtgaa
    11581 gaccggaacc tcgtgcagaa caacctcatc ggcaccaacg ctctcggcct cgcgggtctg
    11641 ccgaacgccg aaggcgcatc gggcatcaac aagggcggcg tgctgatcat cgaggcgagc
    11701 ggcgtgctcg tcggcggcgc gggcgtgggc aacgtcatct ccggtaacaa cgggatcggc
    11761 gtcatcatct ccggcagtat tgcggagggc ggcgcaccgg ccaccgacaa cgccgtgcgc
    11821 ggcaacttga tcggcgtcgc cgccgacggc gcaaccgcgc tcgccaatag cggcgacggc
    11881 atcgagatca cgaacggcca gaacaacttc atcggcggca cagcctccgg tcagggcaac
    11941 accgtcagtt ccaacacggc gaacggcatc gcgctcatcg gctcgctgtt cgtcggcggc
    12001 gagagcagca acaacattat cgcgggcaac accatcgcct caaacggcat ggacggcgtg
    12061 ctcgtctctg cggggacggg caaccgcatc aacagtaact cgatctactc gaacggccag
    12121 caaggcatag acctgctcgg cgacggcgtg acgccgaacg acccgaacga cgcagacccg
    12181 ctcgccggtc tgaacgcgaa caacctgcaa aatttccccg tcgtctcctt cgccgtgaga
    12241 gacacagaga cgggcgtgac gaccatcacc ggaacgctcg acggcggcac gcagggagtt
    12301 gtctatttca tcgaactcta cgcgaaccct gcctgcgacg gaacgaacgg cgaggggcgt
    12361 cttccgctcg gcggcgtcct taccggcgcg gctgatgcga acggggatgt cagtttcacg
    12421 ttccagacga gcgcagacct gagcgggcag gtgattacgg cgacggccac ggacgagacg
    12481 acgaacgaca cctccgagtt ctccgcctgc acggcggtca cgaccaccag cgcgccgccg
    12541 cagggcgtgc ccacgaccgg gcagatcatc atcagcgagt ttcgcctgcg cggcacagac
    12601 ccggacggcg gcggagcgga aaccggcgcg ctcgacgagt tcatagagat ttacaacaac
    12661 accgattccg acatcgtcgt cgtggactct tcgcccctgc ccccgctcac cagcaacggc
    12721 tgggcgattg tctcctccga tgctcccacc acggcgaagt tcgttattcc gtccggcacg
    12781 cgcatccccg cgcgcggcca ttacctcgtt gccaacgccg tcggctacag cctcggcctc
    12841 tacccgtccg gcaacgacgg gagcgtgcag acgacggcta tcgaagacgg cggctacaac
    12901 gtggacattc ctgataacgc aggcatcgcg ctcttccgca cggccaaccc cctgttcttc
    12961 ctgaatccgg ttgaacgtct cgacgccgca gggttcgccg gaggcggcgt cctcttcacc
    13021 gaaggcacgc cgctcacgcc gacgggcggg gtgaccgctt cgctccagca cagcttcgtg
    13081 cgcaggatga cgacggggct gccgcaagac acgaacgaca acgaagccga cttcgagttt
    13141 gtcgaagtga cgggggcaac cagcggcggt cggcgtgccc ggctcggtgc gcccgcaccg
    13201 gagaacctga cgagtcccat ccagcgcaac gccctcatca aggcgatgta cgttgacccg
    13261 caatgcacct tcggcacgcc ggagagcgcg tgcgcccgcg tccgcacggc acagggcgcg
    13321 aacccgaccg acgcagcctt cggcacgctg ctcctccgac gccgcttcag gaacgccacc
    13381 aaccaggccg tcacgcgcct gcgcttccgc ctcgtggaca tcacggcggg cacgcaggag
    13441 agcggcggag ccgacttgcg cgcgctatcg gggagcaacg tgtctgtgac taactcacgg
    13501 ggcgagactt tagaattgaa gggactgacg cttgaagagt tgagcgcgcc cagcccttcg
    13561 cagcctgtcg gcgggggctt gaattcgtcg ctctcggcgg gcacggtcac gctcgcgcaa
    13621 ccgctcgcgg cgggtaactt catcgacgtg gagtttcgca tcggcgtcat gcgaaacggc
    13681 agcttccgtt tcctcgtgaa cgtcgaggct ctgtttgcgg aggcaaaccc cgagacggaa
    13741 ttcggcgggt caaaccggaa gtcgaagggc gcgaagtcca acgtcaggaa gcaggcaagg
    13801 tagagtggga atttagcggc gcggcgtgac gacgggcacg ccgcaaaata aaagcggagg
    13861 ggcgtgtgag tagctactca cacgcccctt caactttcca tgcagggttg aatttatctg
    13921 ccctgtcaca gcgacgactc atctttatga gcgcgcctgt ttcggtctaa tcaacgctgt
    13981 ctaactcgct agcgagtctt ccccgtcggg tcggccttcc cgttagttag tcttccccgc
    14041 tagttattct tcgtcttcgt cttggtgttg gttcccgtgt cggctccggt gccggttccc
    14101 gtcccggtgg tcgtatcctg cgtagacggg ttggtggcag tcgcggtgtt ggtcgccgtg
    14161 gtgtcgcctg tcgcggtcgc cgtgccggtg gctgtgccgc cgccgaccgt gccggtcgcg
    14221 gtggcggtgt tggttgctgt cgccgcgccc gtacccgtcg cggtgttggt ggccgtgccg
    14281 tccacggtct gcgggttggt gccggtggcc gtattggtca ccgttaccgc acccgtgccg
    14341 gtggcagtgg cggtggcggt tctgccggtc accgtccggg tgccggtcac ggtggcagtg
    14401 ttggtggcgg ttcccgtcgc cactccggtt cccgtcccgg tggcggtggc agtacgatcc
    14461 tgagtcgcgg ggtctgtggc ggtggcggtg ttcgtcgccg tgttaacgcc tgtggcggta
    14521 gcggtagcgg tggcggtgcc gtccgtgttt gtgagtctgg tgccggtggc tgttctggtg
    14581 gcggtgcccg tgagggtgtt gttgcctgtc gcggtggcgg tgcgcgtggc cgtcgcgtct
    14641 ccggtcagcg ggttggtggc ggtgccggtg ttggtcgccg tgttgatgcc cgtggcggtg
    14701 gcggtgccgg tggcggtgtc attattgtta tcgccgcctt tgcccttgcc gcttttattc
    14761 ttcccgcttt tacctctgcc gatattaccg gcagccgtct gggtcgccgt cgcggtggcc
    14821 gtattgactc ccgttccggt tcccgtctgg gtggccgtgg cgtcgcgtgt gttgaggttg
    14881 gtggcagtcg cggtgttggt cgccgtgagc gcgcccgtgc ctgtcgccgt gccggtggcg
    14941 gttctgccgt tgcgtgtgcc cgttccggtg gccgtccccg cacccgtgcc cgtgttggtt
    15001 cccgtagcgt cattcgtgcc gaggtcggtc gaggtcgcgg tcgccgtgtt gacgcccgtg
    15061 cctgttccgg tggccgtgtc atctatgtcg ttatcgtcgt ccgcagagcc gtccttggag
    15121 ggagtgacaa acgaatcgtc gtcgctggca aaagcactga acgccccggc ggagagtcct
    15181 accgccagag tggcgaaaac cgcgccgaga cggacgccga gcggacgcct accgaactcc
    15241 cgccccgtct cttcatcctc gatgagttgc aggtaaacgg acgacggcga tcttttcgca
    15301 gcgtcgttct tcccgatcag actttctctc tcgctgaccg ccggagcgcg gcggcctagt
    15361 agtcgtttca tgctgagcat ctcgatattc cccctgtagt ttactgctgt tgagcgaacc
    15421 ggtgtgccgg gcaccgcagt taaatcaaat ttgcgacagt caacggacgt gtatcccgcg
    15481 ccgcgtggct ttgagacgcg gcgaaattag agcgacgagc ggttcaagcg acgtggcgac
    15541 ttcggaggcg gcgggacgct cagccgggtc tttagccagc atgcttgaaa ttagttcctg
    15601 taggggttgg gggacgtgtt tcgggagcgg cttcggctcc gagactaatt gcgggaaacg
    15661 cagcgtgcga tccctgctct tcgcctcgcc gcgcgggaag ggcgtttcgc cggtcgtcgc
    15721 atgatagagc gtggctccga gtccccacac gtctgcggcg tgcccgacgc tgccggggcg
    15781 ggcagccggg tcgcattgtt cgggtggcat gtacgggtcg gttccgattg agtggcgcag
    15841 ttgggctgcg ctctcgaatg attgtgcgat gctgaggtcg atcaggcgcg gcggaactcc
    15901 catcacgata ttactgggct tgacatcgag gtggacgaac tcttcgttcg ccatgtactg
    15961 gagaacggcg gcgacgtgca gggcgagcgg cagcagttga tcgagcggga tggtcgtctg
    16021 ttgcttgatc aggcggcgca gggtgggtcc ctctagatgt tcgagcatca cgtgcgggtg
    16081 agggctatcg agcacggcgt cgaagcagcg caccagacag gggtgcgcga ggcgtcggag
    16141 cacttcgacc tcgcgctgaa gttcgccgcg agcgtgctcg tcttcggcct gatcgggtcg
    16201 cagcaccttc gtcacggtaa tggcgaagag ccgttcatcc cacgtcagga agacctcgta
    16261 acgattcccg ccggagagcc gtttcagcac cgtgcggcct tctgcgatct gcgccccggc
    16321 ctcgaagttc caactcgcct tttcctcttc ccgtcgttta ctgacttcgg agccgtgttg
    16381 actactctcc tccgccgccg gagcaacctc ttcatcgctc tgcctgccgc ccggctgagc
    16441 cggacgcacc gcatgccaac gatccttgag ccaggaggaa cttctggaaa ccggcgagga
    16501 gaacatctat cacctctgat ccgcagttgc gcgaatattc ttctgctttt gaaaagtttt
    16561 cgataacggt tggccggaag agagcgcgca ggaacgcgca atctctatgg agccaaacgc
    16621 ctgctttgag gagagggctg aactttacgt taaatttgat aatcagagta ttgtttttct
    16681 attacatctt tctcccgctt tcctgtgaga gccgcacgcc cttcgtcccg ccctctctca
    16741 tcaattattg cgatagcgtg aagatcaaat tctaaattgc tccggcgcaa ccgatctgcg
    16801 cgtttttgcc atctagtagc ttgactctcc tttaaggttt gaagagaatc gcaggcgatt
    16861 cagggaaacc aaaaacaagt acctcgtgtg ggtttggtca gtacggttcg acgtgggcgc
    16921 gcccgacaac tagcgtccgg tgcgccgcag catgtggtgg ctgcgtgcgc attttctttg
    16981 tcgggctgaa gcaaagcctc gcgcgcttcg ctatgaagag agacgcaccg gcatctctga
    17041 aggagagcac cagcagcagc aacagtccga aaattttatc actcagttaa gtgggagaaa
    17101 gaaaacaatg agcgtgaaca agtcgaagag attccaagtg tcaaccatcg ccctcaccat
    17161 cgttttagcg ttggcctccg ccgccttcgc acaggacgcg acgggcagca accagaccac
    17221 cggcagcgcc aactcgcgta gcgtcgccag cggccagaag atgaccgtca aaggcgtcgt
    17281 caccagccgc gacatcgacg ccgacacctt cgtcgtacag gacagcaacg gcgtcagcac
    17341 caccgtgcgc ctgaccgacc gcaccagcgt taaatcgaag ggcggcttct tcggcggcgg
    17401 ttcaacttac ggtccctcga acatcctgcg cggcctcaac ctcgaagtcg aagggcgcgg
    17461 cgacgcttcg ggcaatctcg tggcggagaa agttcgcttc gggagcacgg acttgagagt
    17521 ggcgcagtcg attcaggcgc gcgtcgatcc ggtcgagtcg cgcgtcggca cggctgagaa
    17581 ccgcatcggg caggtcgaac agaattcgca acgcctctca ggccagttgg atgaactcgc
    17641 cgccgtctcg aacgcggcgc gcggcggcgc gaaggccgcg caggagtccg ccgatgccgc
    17701 tatcgcaggc gtcaacgtga cgaacgaacg catctccgcg ctcgacgact acgtggcgca
    17761 gcagaccgtc gccgtcaact tcggcgtcgg cagcgcggtg ctcacgcccg cttcaaagac
    17821 gacgctcgac cagatcgcgc agcaggcttt gaaggcgcgc gggtatgtgc tggaagtgcg
    17881 cggctttgcg agcgcggaag gctcggaaga gaagaaccgc atgctcagcc agcgtcgcgc
    17941 cgatgccgtc gtgcgttatc tggcctacac gcacaacatt cctttgcgtc gcatcgtgac
    18001 gccgttcggt tatggcgagc agcaggccgt cgccgataac acgacgcgca cgggtcgcca
    18061 gcaaaaccgt cgcgtggaag tgagcgtgct cgtcaatcgt ggtctgatgc agaccgcgcc
    18121 cgtcatgagc acggcgaagc cggacgcggc gacgacgccc gaccagcaac ccgaccaaca
    18181 ataagctacg tgtaacgcga cacagggcag agggaaaggg ggaagggcga aggttaaggg
    18241 aagacagccg cggcttcctc tttttccttt gccctttttc ctttaacccg cgccccgtct
    18301 acgtcgaagg gtggcctcga tgaactcaac tcaaaacaga ctgcgcgcgc tcctcctgct
    18361 actctcgatc tttcccggcg ggacattgcc gctcgccgcc gctccgcgct tcgagtcgaa
    18421 agcggagacg agcgcaggcg tgcaaaccgc ccgcagtagc agcaggcgtc gccccgctcc
    18481 ggcgaagacg acgccgaagc agcagactcc cgcaacgcaa cccgtacgcg tcccgcaact
    18541 cgcgccccgg acgacgcagg cgacgcaggc gacgcagacg ccgcaagcga cgcccgtacc
    18601 cgtgccgacg cagacgccca caccgcagcc gcgcgccacg cgcccgcccg tcgcagactt
    18661 cgggactccg ccgcagaaga cgaagcccga caacgataaa gttcaggatc aggtgacgga
    18721 cgacgccggg gcgatcagcg acgacgatga gatcgtccgc gtcacgtcga atctggtggt
    18781 cgtgcccgtc tccgtgacga acgagcgggg cgagcctgtg cagggattga aggcgaccga
    18841 ttttcgtctg gaagaagaag gacgcgcgca ggagatcgcg catgtgagcg acgcggatca
    18901 agtgccgctc gacatcgcca ttctcttcga cgtgtcgagc agcgtcggcc agcgtttcgc
    18961 gttcgagcag gaggccgccg cgcgttttct caaacaggtc ttaaagccga ttgatcgcgc
    19021 cgccgtcttc cgcatcgaag agacgccgcg cctcgaacaa caactcgcct ccgcagagat
    19081 cgcttcggcc accctgctca agattcccgc gccgcagaga ccgacgccca ccgccttcta
    19141 cgattcgacc atttcggcgg cgcgttatct cgccgccaac gcccccggtc gccaccggcg
    19201 cgtcatcgtc gttatctcag acggcgacga caactacagc gcgcgtgtga aggaaggcga
    19261 agtcgaagag gcacgcgccg taaatcgcgg cgagaagttg cccgccaccg cgctcacgcg
    19321 ccagcgcgag acgcaccgca aggctctgct cgaagtgcag cgcgaagtgc agcgcgccga
    19381 tgcagtcttt tattccatca acccaagcgg cggcgggcta cagttcaacg cacgcggcgc
    19441 acgcgcacag gaaggcatgc agcaactcgc cgagacgacc ggcggcacct ccttcaaacc
    19501 cgaacaactc gaagaactcg acgacatctt tcgtcgcatc gccgccgaac tccgcgcaca
    19561 gtatctactc caatactatt ccacgagcga cgcacccaac ggcaagtttc tcaacatcaa
    19621 agtccgcact cccacacgcg ccgacgcacg tgtccgcgcc cgctcaggct actacgtgaa
    19681 aaagtgatga gtgatgagta atgagtgatg agtgagaaga atttttaact catcactcat
    19741 tactcatcac tcatcacttt ttcacttgtc actgtcctag tgcatttcct gcaacacgtc
    19801 tgtcaggcgg ttgatgactt cgaggaggga gcgaccggct gcgtcgaggc ggttggagga
    19861 ggcttgctgt tcggcgttgg ccgcgccgag cgcgacgccg gacagcctga tggagaagcg
    19921 ttcgagttcg ggcgtggtgc ggaagtgtag ttccaacccg tcgagtcctt cgcgtatgtc
    19981 ctgtaggctg ctgatgagac ggttcttcaa ggcgctcgcc gtcgcatcgg gctgcccgcc
    20041 ctgccccacc gccgcctcga aatccttcga gaagcgtccg tagagataga cgaaatttga
    20101 gatctgtttg atcttttcgc cgacgcgctg ccgcccgtcc tgcaaggcga gagcgttggc
    20161 ggcagtgctc gcgggtcttc tgccgcgcgt cgccgtggga cgcttcggcg cgctcgcgcg
    20221 ccgctgtcgc ggctgcgcga aattttcgag gggcgtgaga agcaacaata ggccggttag
    20281 ggtcagcgaa taaacgaatc tcaaaatagt ttctccttga tctgtagacg gaagagagag
    20341 ccggatgacg acaaactatg cccgcgcgcg tggcggcgtc aagttgcaag cgcgcccgta
    20401 gcgtgattcg gcgtcgcggc agccgcgctg ctacaatgtg cccggactac aaagcaaaaa
    20461 cttgaaccgc cgattctttg cgatgccgaa aagaacgtta caggttcgcc gcctcggacg
    20521 gatggagtac ggcgcggcct acaaaattca gaaggagatc gaagccgcgc gcaaaacggg
    20581 cgcgggcgcg gatactctcc tgctcgtcga gcacccgcac gtcctcacgc tcggacgccg
    20641 cgccgacgaa tccgccgtgc tcgccgcgcc cgaaactctg gcgtcgcgcg gcgtcgcgct
    20701 ctttgagacg aatcgcggcg gcaaagcgac ttatcacggg ttgggtcaac tcgtcggcta
    20761 cccgatcatc aatttgagtc ccgaacgcga agatgtgcat cgctacgtgc gcgaactgga
    20821 agaagcactg atcgccgccc tcgcggattt ccgtatcgag agcttcagga tcaaaggctt
    20881 gacgggcgtg cacacggcgc gcggcaaagt cgccgccatc ggcgtccaca tctcgcgctg
    20941 ggtgacgacg cacggcttcg ccttgaacgt caacaccgat ctcagtttct tcgacttgat
    21001 catcgcctgc gaaggcgaac ccgtcacctc aatgtacgaa ctgctcggcc acgagttgcc
    21061 cctgcgcgaa gtggaagacc gcatcgtcgc gcgcttcgcg gaagtctttg agatgacggt
    21121 aacgccggaa gatgaatatg ctaacgcttg atttcatacg cgaggcgcac gcgcgcatcc
    21181 gttcacgcgt gcatcgcacg cccgttatct cttcgcgcgc cttcaacgag gcggcggggc
    21241 gagaggtctt tttcaagtgc gaaaacttcc agcgcgccgg ggcgttcaag atgcgcgggg
    21301 cgacgaacaa gattctcacg ctcgacgcgg aggaacgcgc acgcggcatc gtcgctttct
    21361 catcgggcaa tcacgcgcag gccgtcgcgc tggcggcgcg cgatgcgggc gcgcgcgccg
    21421 tgatcgtgat gccgcaggac gccccgcgcg cgaaggtggc ggccacgcgc ggctacggcg
    21481 cggaagttat cttctacgac cggcagcgcg acgaccggga agccgtgacg cgcgatgtgg
    21541 ccgaacgcga agggcttgtg ctcgtgccgc cttatgacga ctattatgtg atggccgggc
    21601 agggaacgtg cgcgctggag ttgcttgagg agactgctga actggatgcg ctcgtcgtgc
    21661 cttgcggcgg cggcggtctg tttgcggggg cgagcgtggc ggcgaagggc atccgccggg
    21721 agatactctg tttcgccgtc gaaccggaga cgggcgacga cacgcggcag tctttccggc
    21781 agggcgagcg cgtgtcgatc ccgccgccgc cgaccatcgc ggacggcgca cgcctggagt
    21841 cgccgggcgc gctcacgttt cccttcgtgc agcagcacgc ggaggacgtg ttgaccgtct
    21901 cggatgcgga actggtcgag gctctgaagt ttctcctctt tcgtttgaag atactcgtcg
    21961 agccgacggg cgcgctcggc gtcgcggcgg tgctttttaa taaacttccg gcaaacattc
    22021 gacgcatcgg agttgtaatc tccggcggta acatagatgc agacgcgctg gcgcggctcg
    22081 tgcaggaaga gtagccgcgt agaaaccctg cgacaggcgt tagacgtttc aacttcgcgc
    22141 cagaggaaag ccggaagcag gacactacga actcagggag ttagtgatgg acaaaaacaa
    22201 ttcgctcaaa gagcctttgc aggtggagaa gaatgctccg gtgcgcaacg tcgtgcgctt
    22261 ctggttcatc gcgctcgcgt tgatcattat aggcttgctc atcatcggcc tgatcttcgg
    22321 caacttcgga atacagaggt gacgggcggg cgacaggcga ctccggcaag tgttaaaata
    22381 gccgcaagtc aaaaatcatt gtggagcgaa tttaattgag ccgcgagtta aaagtcatca
    22441 gcaacaatcc gccgccgccg cagggacgcc cgacggggcg caagcccgac tggttgaaga
    22501 ttcgcatcgg cgacccgacg aatcagaacc acgtgttcaa tttgatcgag gggttgaacc
    22561 tgcacacggt ctgccaggag gcgcgctgcc cgaacatctt cgagtgctgg tctgaccgca
    22621 ccgccacctt catgctcggc ggcgacatct gcacgcgcca ctgcggcttc tgcgccgtcg
    22681 gcaaagggca gcccggcgcg ctcgaccccg atgagccgcg ccacgtggcc gaagccgtgc
    22741 gccatctcga tctcgcctac gccgtaatca cttccgtcaa ccgcgacgac ctgccggacg
    22801 gcggcgcagg ccactgggcg cagacgatac gcgaagtgcg cgcggccaac cccgcgtgca
    22861 agatcgaagt tctgattcct gatttcaacg gcgacgaggc cgcgctcgat acagtgctcg
    22921 acgcacagcc ggacgtgctc aatcacaaca ccgaaaccat cgcgcggctc taccgtcgcg
    22981 tgcgtcccga tgcgaagtac gcgcagacgt tgacgctgct tgagcgcgcc gcgcgccgac
    23041 gcgacgcgga ggagcgcggg atgctgacga agagcggcat catggtgggc ttgggcgaga
    23101 cgttcgagga agtcgtcgag ttgatgcgag atttgcgcgc cgtctcatgc gacatcatga
    23161 ccatcgggca gtatttgcaa ccgcacgcgc ggcgtctgcc ggtcgaacgc tacgtgacac
    23221 ccgaagagtt tgcgcgctgg cgcgacgtag ggatgtcgat gggctttcat cacgtcgagt
    23281 cgagtccgct cacgcgttct tcgtatcacg cgcggcagca ggtggaaggc actgcgcagg
    23341 aagagacgaa gcgcgacgaa gaagccgtcg ccgtgagtta accgccggac atttcgccgg
    23401 aaagcacgag gacacgaagt ttactgcttc gtgtcctcgt gctttcctgg ttgcgtctac
    23461 aaggccaacc gctcgaagtg ctttatccgc gcaagtcagg ccgcg
//
LOCUS       DASWNS010000241        15829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_164449, whole genome shotgun sequence.
ACCESSION   DASWNS010000241 DASWNS010000000
VERSION     DASWNS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15829
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164449"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VGB76_08315"
     CDS             complement(<1..1077)
                     /locus_tag="VGB76_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY0098945.1"
                     /translation="MSHFPRPQNPRAHLRAHAFALMLVLACGLLLMPAAARAADTEYT
                     GKLDAELVANREDLDQVILKPLRDLSKVKFATPLDAAGAHITASRLHHPGQDKSVILA
                     LLVEVEDETPQLYADLNLDGLMSDNERFALTRGEDGSPYILEATLKLPLTGGLFNNFP
                     LYVQYLRNVRWNEMAEDERMLLQSKTAYARGQVDVAGKKTLVQFGYKPQSKKISATNG
                     WVGIDGDGDGQIDMDRFSPEAAEARDETIVFRVGGEYVSAKRVDVEKNIILLRTHAPS
                     DYKRVELRIGGEVPDFAFTDFAGKKRRLSEFRGKYVLIDFWGLWCPACRTELPYLKLA
                     YSRFQARGFEILGMNTDQPEMIPSL"
     gene            1414..1872
                     /locus_tag="VGB76_08320"
     CDS             1414..1872
                     /locus_tag="VGB76_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098946.1"
                     /translation="MATKKAASQGANKGETAERSRAAKKIDASRNGGASKKLGANERI
                     IHVPASQGKCTDWQAVQDSRPPLVPRLRVTGRCTFPTPGYKVTLKESVPQGTNPRILL
                     LTKTVKPPRGIQPQVLQTILVGYEKKNATTTYDSVTILPEVKTIKVKQVT"
     gene            2340..3725
                     /locus_tag="VGB76_08325"
     CDS             2340..3725
                     /locus_tag="VGB76_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEY0098947.1"
                     /translation="MNKIRLTVALMLVFALLSVTPATFAQRRRPVAPPRRTTPAPRRT
                     TTRPPARTTATVADTRLTGLYRLDFSRSDDPRAAAERATTFLLPEEQARVIESLTARL
                     TSPDQIAIERRGQNISIGSTRAPRVNFDADGRTRTEQAEDGHTVTTRAALYGDQLMIS
                     SSGSHDDEFSVNFDSIDNGTRLRVTRRIRDERVAQPVVVQSTYNKISNVARFSIYGEP
                     ESARTATNNTARNAPPRNLPPARRTGTTPEQPRAQQTPQPQRRSTPPVIRNREPRAQQ
                     PPAPAYEGFVIGNNTQFITTLDNNLSTDGSREGDRFTLTVREPSVFAGAIIEGTVSRL
                     SRGGRVSGRSEILLNFERIRLRDGRTADFNAFIESVHPLGGEEVRVDNEGGAGVQEGD
                     SQTARTTQRAAIGASIGAIIGAISGGGKGAAIGAIIGAGAGAGSVYAQGRDDLELRSG
                     TELVLRAAGAR"
     gene            4393..4812
                     /locus_tag="VGB76_08330"
     CDS             4393..4812
                     /locus_tag="VGB76_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098948.1"
                     /translation="MTDILKLEGRVLGEGTNNKTVGQHKVKSYRVEEVTLPRMQEVKI
                     KGETRSVGRAFRVTIKGGPFPVRAMPAVIWLDETAIGYGIENEELTEITAITYDEAAL
                     TEGATIYLSYGDKKNKEDRSALPEKLKLNGVAKGGNQ"
     gene            4812..6569
                     /locus_tag="VGB76_08335"
     CDS             4812..6569
                     /locus_tag="VGB76_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098949.1"
                     /translation="MKTSHLNRLIAFAFLMFLSVASASITQAQTPDPGTTGPNAVTRE
                     EYNYGNSVFQPTGFPIAVEVRASIHYPTSLSGGPYPVIVFMHGRHATCYRSSTAALRW
                     PCRSTEQPIPSFQGYDYIAQTLASNGYVVVSVSANGINAYDNNVNDLGMQARAELIQH
                     HLGRLNTFNTTGATPFGTKFVGKLNLSKVGTMGHSRGGEGVVKHYVYNASLGSPYTVK
                     AVLPLAPVDFNRPVVNNAALEVILPYCDGDVSDNQGVHFYDDARYNVAGDTGAKHTIQ
                     VMGANHNFYNTVWTPGMFPAGTADDWGYVTGGSTDGHCGTGATNKRLTAAQQRGTGLA
                     YLSAFMRVYVGGESQFLPLLTGAAPPPASAQTTNLHVSYHAPDTATTRRDVNRLLTST
                     NLTTNTLGGATTQSGLTPHDLCGGNSPQPLHCLPSTQATARQPHTTVSARSTLRGLSQ
                     LRTGWNAFGSTYTNNLPVGTGNVSGFQALQFRVSVNFSDTRNASNTPQNFSIVLTDGA
                     GATSTVRVGDVSGALFYPPGSVGPVPKVLLNTVRVPLSSFTGVNLTDIRSVKFNFDQT
                     GAGAVLISDLAFASAPTVP"
     gene            complement(6639..7013)
                     /locus_tag="VGB76_08340"
     CDS             complement(6639..7013)
                     /locus_tag="VGB76_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0098950.1"
                     /translation="MPQPVILYVENNMFLMQLVRDVLDLAGWNVQHLRSATSARTLLR
                     SEQPYDLLLVDNELQYMSGIELVRCARESAHRKGLQILLFSIEDYEEEAKRVGADAFL
                     RKPHDLHLLLDTIRRLLAAGGK"
     gene            7293..7610
                     /locus_tag="VGB76_08345"
     CDS             7293..7610
                     /locus_tag="VGB76_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0098951.1"
                     /translation="MGNARRRPKRLAEKLLQIRNSLGLSQPELYSRLGVEDEITYHRI
                     SEYERDKSEPTLMVLLQYSRVAGVHLEDIADDELDLPEKLPGNYRHEGIRRKPSSGSK
                     SKR"
     gene            7708..8043
                     /locus_tag="VGB76_08350"
     CDS             7708..8043
                     /locus_tag="VGB76_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098952.1"
                     /translation="MTSEELERAIEFLLKSQAAFEASLAASKEAFEERQTAFEERQTA
                     FEERQAAFEANQGTFSARQERTQEHLDHLTRVVGNIAEITQRNSEDIDVLVKLVGGII
                     ERGNGKTEK"
     gene            complement(8357..9214)
                     /locus_tag="VGB76_08355"
     CDS             complement(8357..9214)
                     /locus_tag="VGB76_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098953.1"
                     /translation="MLKISRRRILLVVLALSVIATFAAVTSANHSWNGYHWARTSNPF
                     TLRVGKNLSSAWGTAYSGSISDWSASSVLNLSSYTGSAGSACGMVRGTVQVCNGSYGN
                     NGWLGIASINLLSGTKHITQGSAKMNDTYFNTATYNNPNERRHVMCQEIGHTFGLGHT
                     SENGTSQNTCMDYFSNTGANATSTVSTRPNSHDYALLESIYAHLDGSTTVGARTTSTA
                     HEAGEDAGSDITEDPISWGHLVSQSHNGRSSIYERSNPNGSKTITHVYWTEEAAENCH
                     HCDHRHDRH"
     gene            complement(9742..10323)
                     /locus_tag="VGB76_08360"
     CDS             complement(9742..10323)
                     /locus_tag="VGB76_08360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY0098954.1"
                     /translation="MAVLKKIRSRLFAPSKEASANGDEAQGENRATEGATQASSLPEV
                     RYDIRPLTISQLDECWRLDQRCFVDGEAYSRDTFEYLLTSPESVAYRAVTPDGLMVGF
                     IVGLVEPQNTGHITTLGVAPEHRRRGIAAQMLVKVEENFRRRHVRTIRLEVRSVNSGA
                     QNLYRNLGYTVTQRLPRYYSNGGDGLLMLKSVE"
     gene            complement(10464..11528)
                     /gene="queA"
                     /locus_tag="VGB76_08365"
     CDS             complement(10464..11528)
                     /gene="queA"
                     /locus_tag="VGB76_08365"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY0098955.1"
                     /translation="MLISEFDFELPEELIAQAPLAERDASRLLVLDRVRASWRDSSFS
                     QLPAELREGDQLVVNNTRVFPARLVGRREPSGGRVELFLLAEREPSVWDALARPARRL
                     ARGQELSFGGGRLRAEVIDARDDGQRTVRFDCAGDFHALLEEIGQVPLPPYIKRADEP
                     DASSLSQQRTADRERYQTIYAERLGAIAAPTAGLHFTPRVLEALRARGVCVTEITLHV
                     GYGTFAPVRVNDLSEHAVAAERYEITPEAAATLNDARSSGRRIIAVGTTSVRALESAT
                     DDSTGRLTPGARSATLTITPGYRFRSVDALVTNFHLPRSSLLVLVASFAGRELTLAAY
                     RHAVAARYRFYSYGDCMLII"
     gene            complement(11650..12591)
                     /locus_tag="VGB76_08370"
     CDS             complement(11650..12591)
                     /locus_tag="VGB76_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY0098956.1"
                     /translation="MKRLRSFTRQRFGVRNAAPWLNIFGHSFHQFHENDLFTPAAAMS
                     YFGLLTLFPMLLVLLALSNYFDVGSEMLTRAVEAYPGSRDFLRTTVRSLENVSTSVIL
                     TCIIVTLWAGSWVFNVMERAINRLWGTKPRAFLHGRGLTLGMMGGVGVLLIASVMITY
                     TLVALKELAERLPLRVLQRVPILSLVGNAFWQLVFALVSIAVTVALFTLVYRFMPSGR
                     VNLQDSIPGAVVAGLLWEAAKFIFASSLHYFHYDQIYGSVGAVVAVLTWSYVSSLILL
                     FGAQLSVVFHQEHSTAHEELADTVPPGETQTPAMPQA"
     gene            complement(12609..13646)
                     /gene="ruvB"
                     /locus_tag="VGB76_08375"
     CDS             complement(12609..13646)
                     /gene="ruvB"
                     /locus_tag="VGB76_08375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY0098957.1"
                     /translation="MSEELEPASVRLSAPTDEESQFELSLRPARLAEYIGQRKATENL
                     RIFIRAATMRREALDHVLLTGPPGLGKTTLANIVANEMGAELRSTAGPVIEKAGDLAA
                     LLTGLQEGDVLFIDEIHRLNPAIEEVLYPAMEDYQLDIMIGQGTAARSIKLPLPKFTL
                     VGATTRAGLITAPLRGRFGIVFHLDFYKHDELELIVRRSADILGTEIDEGGAKEIARR
                     ARGTPRIANRLLRRVRDFAEVDYEGRITQEVAHDALNRMEVDTFGLDEVDRKLLLTII
                     EKFSGGPVGLGTISASVHEEKDSIEEIIEPYLIQIGFLNRTPRGRMATRLAYEHFGLS
                     PTPSNPGLFGV"
     gene            complement(13738..14358)
                     /gene="ruvA"
                     /locus_tag="VGB76_08380"
     CDS             complement(13738..14358)
                     /gene="ruvA"
                     /locus_tag="VGB76_08380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY0098958.1"
                     /translation="MIAHLSGTLLAKQATSVILDVGGVGYEVTIPVSIFYDMEEPGAN
                     ISLRIYTHVREEALQLFGFKTARERELFTLLISVSGIGPKSAIAMLSGMNADEIISAI
                     RSNNLARLTSIPGLGKKTAERLVIELRDKMTTLSHPALEEQLAAQKAGAAPSEDTLRE
                     DTLSALTNLGYQRAMAEKVINQAIQEGGELSVELLLRRSLKSLSKG"
     gene            14617..15324
                     /locus_tag="VGB76_08385"
     CDS             14617..15324
                     /locus_tag="VGB76_08385"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEY0098959.1"
                     /translation="MKSEKISELTTNRLSVYLRCLNKLAAAGIKTVSSRSLAEDFNLN
                     SAQIRKDLAYFGEFGTRGVGYTVNDLRAHITKILGLDTEHRVGIIGAGHLGTALANYK
                     GFTASNFAVVALFDNDRERIGQTVGDARITIYDVRQIARVVRAEQIDVAVLAVPARAA
                     QKVLNQVMSAGVKAVLNFAPARLHARPGVKLKTVDLTISLESLSYFLARPAASSTTNA
                     RKVLSFEELLQPEENEL"
     gene            15349..15666
                     /locus_tag="VGB76_08390"
     CDS             15349..15666
                     /locus_tag="VGB76_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098960.1"
                     /translation="MSEDLTQNLPDTFQERVLAELTAIRQENARLSVQVISIDTRLTS
                     LEDKVDARLRETRPIWEGVLERLTEIEKGLSTLNRQFKTMIRGEFDLHTRVEKLEDDQ
                     QAV"
BASE COUNT         3222 a         4763 c         4833 g         3011 t
ORIGIN      
        1 gagcgacggt atcatctcgg gttggtctgt gttcatgccg aggatttcga agccgcgtgc
       61 ctgaaagcgc gagtaggcga gtttgagata gggcaactcg gtgcggcacg ccggacacca
      121 cagcccccag aaatctatca acacgtactt gccgcgaaac tcggagagcc ttcgtttctt
      181 gcccgcgaag tctgtgaagg cgaagtcggg cacttcgccg ccgatgcgca gttcgacgcg
      241 cttgtagtcg gagggcgcgt gtgtgcgcag caggatgatg ttcttctcca catcgacgcg
      301 cttggcggag acgtactcgc cgccgacgcg aaagacgatt gtttcgtcgc gcgcttcggc
      361 agcctcgggc gagaagcgat ccatgtcaat ctggccgtcg ccgtcgccgt caatcccgac
      421 ccaaccgttc gtcgcgctga tcttcttcga ctgcggcttg tagccgaatt gcacgagcgt
      481 cttcttgccc gccacgtcca cctgcccgcg cgcataagcc gtctttgatt gcaggagcat
      541 ccgctcgtct tcggccatct cgttccaacg aacgttgcgc aggtattgca cgtagagcgg
      601 gaagttgttg aacagaccgc ccgtgagcgg cagcttgagc gtggcttcga ggatgtaggg
      661 gctgccgtct tcgccgcgcg tgagggcgaa acgctcgttg tcggacatga ggccgtcgag
      721 gttcaggtcg gcgtagagtt gcggcgtctc gtcctccact tcgacgagca acgcgaggat
      781 gacagacttg tcctgccccg gatgatggag gcgggaggcc gtgatgtgtg cgcccgctgc
      841 gtcgagcggg gtggcgaatt taactttcga caagtcgcgg agcggtttga gtataacttg
      901 atcgaggtct tcgcggttgg cgacgagttc cgcgtcgagc ttgcctgtgt attccgtgtc
      961 ggcggcgcgt gcggcagcgg gcatgagcag cagcccgcac gccaacacca gcatgagcgc
     1021 gaacgcgtgt gcgcgcaggt gtgcgcgcgg attttgggga cgagggaagt gagacatcct
     1081 ggtagttcct ttttggagcg tgtagagact tgggaaacga gccgggggat ggctgcgcgg
     1141 cttctttgac gggcgagatg gtaagccagc cgcgcacgga ctgtcaaaga tataagctcg
     1201 acggtagcgc cgcgtctaat ccgttgcgca ccggcgcaga cgcaagggag cgcagaggaa
     1261 gtggagcggg cagagcagat gaagagacgg ctttctctct tttatattga caacgacgcg
     1321 tctccgcgca tatcgtgccc gcgtttaatc gctggcgatg tcctcgcctt tgctggcctc
     1381 ataaacccac aacaaaggag atggaataac ctcatggcta caaagaaagc tgcgtctcag
     1441 ggcgcgaata aaggcgagac ggctgagaga agtcgagcgg ccaagaagat cgacgcgagc
     1501 cgcaacgggg gcgcgagcaa gaagctcggc gcgaatgaga ggatcattca cgtccctgcg
     1561 agtcagggca agtgcacgga ctggcaggcg gtgcaggata gcaggccgcc gctcgtgccg
     1621 cgtctgcgcg tgacgggcag gtgcaccttc ccgacgcccg gttataaagt cacactcaaa
     1681 gagtccgtcc cgcagggcac caacccgcgc atcctcctgc tgaccaagac cgtcaagccg
     1741 ccgagaggga ttcagcctca agtgttgcag accattctgg tcggctacga gaaaaagaat
     1801 gcgacgacga cttatgacag cgtcaccatt ttgcccgaag tgaagaccat caaagtcaag
     1861 caggtcactt aaagcacgcg cccgttatag acgcaggacg ggcgacgttt gatcgcccgt
     1921 tcaggttctc aacattcgtc gtctccggcg cgggtggata acgctcacgc cgtagacgac
     1981 cgcatcccgc cttctccctc tgtagcctcc agggcgcggc aacgcctcac gcagcggcgt
     2041 tagccagaag cctccgccac tctaaattca ccggcgcgaa actcgaaaaa acttccgctc
     2101 gccgagtctc tctgcacgcc cgtgcatgac ggcggcgcaa ccgcgccggt tctggcctcc
     2161 ggcttgcttt aaagagcggg cacaagttta ttttccgtta agcgacagga gtatttccct
     2221 tgaatccgcg cctccccctg tccggcgcgg cgcgcggcgt agtgaaaacc tacaccgtcg
     2281 ctgaccgcat tcgataacac ttcgacacaa gttttccgct gccgtggagg gacaagcagg
     2341 tgaataaaat cagattaaca gtcgcgttaa tgttggtctt cgcgctcttg agcgtcactc
     2401 cggcgacgtt cgcgcaacgc aggcgtcccg tcgcgccgcc gcgccgcacc acgcccgcgc
     2461 cgcgccgcac aacgacgcgt ccgcccgcgc gcacgacggc gacggtagcc gacacgcgcc
     2521 tgaccggact ctatcgtctg gacttctcgc gcagcgacga cccgcgcgca gccgccgagc
     2581 gtgcgaccac ctttctcctg cccgaagagc aggcgcgcgt catcgagagt ttgacggcgc
     2641 gactcacttc acccgaccag atcgccatcg aacggcgcgg gcagaacatc agcatcggct
     2701 cgacgcgtgc gccgcgcgtc aacttcgacg ccgacgggcg cacgcgcacc gagcaggccg
     2761 aagacggcca taccgtgacg acgcgcgccg cgctctacgg cgaccagttg atgatcagtt
     2821 ccagcggcag ccacgacgac gagttctccg tcaacttcga ttccatagac aacggcacac
     2881 gcctgcgcgt cacgcgccgc atacgcgatg agcgcgtcgc gcagcccgtc gtcgtgcaga
     2941 gcacttacaa caagatttcc aacgttgcgc ggttcagcat ctacggtgag ccggagtccg
     3001 cgcgcaccgc gacaaacaac accgcccgca acgccccgcc gcgcaacctg ccgcccgcgc
     3061 ggcgcacggg gacaacgccg gagcagccgc gtgcgcaaca aacgccgcag ccgcagcgac
     3121 gctccacgcc gcctgtcatc aggaatcgag agccgcgtgc gcaacagccg cccgcgcccg
     3181 cttacgaagg tttcgtcatc ggcaacaaca cgcagttcat caccacgctc gacaacaatc
     3241 tgagcacgga cggctcgcgt gagggcgacc gcttcacctt gaccgtgcgc gagccgtccg
     3301 tctttgcggg cgcaattatc gaaggcactg tctcgcgcct gtcgcgcggc gggcgcgtct
     3361 ccggtcgctc cgagatattg ctcaacttcg agcgcatacg cctgcgcgac gggcgcacag
     3421 ccgatttcaa cgccttcatc gagagcgtgc atccgttggg cggcgaagag gtgcgtgtgg
     3481 ataacgaggg cggcgcgggc gtgcaggagg gcgatagcca gaccgcacgc accacccaac
     3541 gcgcggccat cggcgcgagt atcggcgcga tcatcggcgc gatctccggc gggggcaaag
     3601 gcgcggccat cggcgcgatc atcggcgcgg gcgcaggcgc aggctccgtc tatgcgcagg
     3661 gacgcgacga cctcgaactt cgctccggca ccgaactcgt cctgcgcgcc gcaggcgcac
     3721 gctagatgcg aactcgcgtc aaaaggtaaa acttcatcgg ccagtaaaga ttgcgtaccg
     3781 cctcagtcgc gctttaaacg cgacgctccg gtgcgcaatt tttgtgtact atgacggaaa
     3841 ggaaggaggc agcgacatga caggcgaaga gattgacgct ttgttcaaac tcgtcggcgg
     3901 tttggtggag aggaaaaacg ggaaggctga aagttaaaaa atttaccttt tggccgggca
     3961 accttttgtc cccccgttgc atcttaatcc cgctttcaca atagtcgttt gcgtccgcaa
     4021 cagtcgttta cgcacaacag tggtttgcgt cggttttctt caagcagtta ttacgtaaac
     4081 aaacaacctc aagcattcta ccgggaacga tgatttaagg gtcgatccct gtttacgtcg
     4141 cacttcaccc cccattcttg acgggttctt tgcccaccct caccggtggg cactgtcgat
     4201 ccccaactcg gttttttaat caccagaagg agaatattta tgcgccttgc gaattgcaca
     4261 cgtcgccgac gcggatggaa gagcatcctc acgacgacga gttttctatt cctctttagc
     4321 gcgtgcgctt cactgacagc gtttgcgcag gatacccccg ccgcctcccc caattcagac
     4381 caggaagtaa ccgtgaccga catcttgaaa ctcgaaggcc gcgtgctcgg cgaaggcacg
     4441 aacaacaaaa cggtcggcca gcataaagtg aaaagctatc gcgtcgaaga agtgacgctg
     4501 ccgcgtatgc aggaagttaa gatcaaaggc gagacgcgtt cggtcggcag ggctttccgc
     4561 gtgacgatca aaggcggacc gtttcccgtc cgcgccatgc ctgccgtcat ctggttagac
     4621 gagacggcta tcggctacgg catcgagaac gaagaactca cagagatcac cgccatcacc
     4681 tacgacgaag ccgcgctcac cgaaggcgcc accatctatc tctcttacgg agacaagaag
     4741 aacaaagaag accgctccgc gttgcccgag aaattgaaac tcaacggcgt cgccaaaggg
     4801 ggtaaccaat aatgaagacc tcacacctta atcgcctcat cgcctttgct ttccttatgt
     4861 tcctgagcgt cgcgagcgcg agcattacgc aggcacaaac ccctgacccc ggcacgaccg
     4921 gccccaacgc cgtcacgcgt gaagagtaca actacggcaa ctctgtcttc caacccacag
     4981 gctttcccat tgcggtcgaa gtacgtgcct ccatccacta cccgacgagt ttgagcggcg
     5041 gcccttaccc ggtcatcgtc ttcatgcacg gacgccacgc cacctgctac cgcagttcga
     5101 ccgccgcgct gcgctggccg tgccgctcga ccgagcagcc gatccccagt tttcaaggct
     5161 acgactatat cgcgcagact ctggcaagca acggctacgt cgtcgtctcc gtcagcgcaa
     5221 acggcatcaa cgcctacgat aacaacgtaa acgacctcgg catgcaggcg cgcgccgaac
     5281 tcatccagca tcacctcggt cgcctgaaca ctttcaacac gacgggcgct acgcccttcg
     5341 gcacgaagtt cgtcggcaaa ctcaatctct cgaaggtggg cacgatggga cactcgcgcg
     5401 gcggcgaggg cgtggtcaag cattacgtct ataacgcctc gctcggctcg ccctacacgg
     5461 tcaaggccgt gctcccgctc gcacccgtcg atttcaaccg tcccgtcgtc aacaacgccg
     5521 cgctcgaagt gatcctgcct tactgcgacg gcgacgtgag cgataatcag ggcgtgcact
     5581 tctacgacga cgcgcgctac aacgtcgcgg gcgacaccgg cgcgaagcac accattcagg
     5641 tgatgggcgc gaatcataac ttctataaca ccgtctggac gccgggcatg ttccccgccg
     5701 gaaccgccga cgattggggc tacgtcacgg gcggctccac ggacggccac tgcggcacgg
     5761 gcgcaaccaa taagcgtctc accgccgcgc agcaacgcgg cacgggcttg gcttatctct
     5821 cggctttcat gcgcgtctac gtcggcggcg aatcgcaatt cctgccgctc ctgacgggcg
     5881 ccgcgccgcc ccctgcttcc gcgcagacga ccaacctgca cgtctcctat cacgcgcccg
     5941 acacggcgac gaccagacgc gacgtgaatc gtctgctcac ttcgaccaac ctgacgacca
     6001 acacgctcgg cggcgcgacc acgcaaagcg gactcacccc gcacgacctg tgcggcggca
     6061 actccccgca accgctacac tgcctgccct cgacgcaggc caccgcgcgc cagccgcaca
     6121 ccaccgtcag cgcgaggtct acgctgcgcg gcttgagcca actccgcacc ggctggaacg
     6181 ctttcggctc aacctacacg aacaatctgc cggtcggcac gggcaacgtg agcggctttc
     6241 aggcgctcca gttccgcgtc agcgtcaact tcagcgatac gcgcaacgcc tccaacacgc
     6301 cgcagaattt cagcatcgtc ttaaccgacg gcgcgggcgc gacctcaacc gttcgcgtcg
     6361 gcgacgtgtc cggcgcgctc ttctacccgc cgggttcggt cggccccgtg ccgaaagttc
     6421 tcttgaacac cgtgcgcgtt ccgctctcgt ccttcacggg cgttaacctg acggacatcc
     6481 gctccgtcaa attcaacttc gaccagacgg gtgcgggcgc agtgctcatc tccgatctgg
     6541 ccttcgcctc cgccccgacc gttccctaac gcggcgcgcg cagagaaaca gacctcaaga
     6601 ggcgacggag tgaaaatctc cgtcgcctct tttttagtct atttgccgcc cgccgcgagc
     6661 aaccgccgga tggtatccag aagcaggtgc agatcgtgcg gcttcctgag aaatgcgtct
     6721 gcgcctacgc gcttcgcttc ctcttcatag tcttcgattg aaaacaacag aatttgaaga
     6781 cctttccggt gcgcggactc acgcgcgcac cgcaccagtt cgatccccga catgtactgt
     6841 agttcgttat ctacgagcag cagatcatag ggttgctcgc tgcgcagaag agttcgcgcg
     6901 gatgtcgcac tgcgcagatg ttggacgttc cagcccgcca ggtcgagtac atcacgcacg
     6961 agttgcatga ggaacatatt gttttcgacg tagagaataa ccggctgtgg catgactgtg
     7021 ctccgttgct ggcgtgggtg tgtgcgttca agtaaggaat caggtatgag catgatgcga
     7081 gttctgctaa gatgcgggct gttccaggtt tgaaaatccg cgacggtcgc atgcgtagct
     7141 cacctcaacc tcaagcgagt gatgagcatc gccgttgagt ctggcaggcc aactctctcg
     7201 gcgaatccga tacgcaagta cattctatac ggtacaccgt atagaggcaa gtcgtcgcgc
     7261 gcaatccgtg tctcatcggg aggatgctcc tgatgggcaa cgcacgccgc aggccgaagc
     7321 gtctggctga gaaactgcta cagattcgga actctctcgg tctttctcaa ccggagctat
     7381 acagtcgctt gggagtcgaa gatgagatta cttatcaccg aatctcggaa tatgagcgag
     7441 ataagagcga gccaaccctg atggttctcc tccaatactc ccgcgtcgca ggcgtccacc
     7501 tggaagacat cgccgacgac gaactcgacc tccccgaaaa actccccggc aactacaggc
     7561 atgaaggcat ccggcgcaag ccttcgtctg gtagcaagtc gaagcgttga cagttgcgcc
     7621 aacaacagcc tgagccacgc ctctcacgtc gcgccccacc gcgacattct tgtgtacaat
     7681 atccatgtac ggaggcactg aaacgatatg acaagcgaag agctggaacg cgcaatagag
     7741 tttttgttga agagtcaggc agcttttgag gccagtctgg ccgcaagtaa agaagctttt
     7801 gaggaaagac aaacagcttt tgaagaaaga caaacagctt ttgaggaaag acaggcagct
     7861 tttgaagcga accagggtac tttttcggcg aggcaggagc gcacgcagga gcatctcgac
     7921 cacctaaccc gcgtcgtggg gaacattgcg gagataacgc agcgcaattc ggaagatatt
     7981 gacgttttgg tgaaactcgt cggcggcata attgaacgcg gaaacggcaa gacggagaag
     8041 taaagtcgct tgtcgcaaat ttgcgctcgc ggggtttgtc atcccgacgc gagcgcaagt
     8101 tcccctgtag aaaatcctct cctgaaaaat cgacgaaaga taattataca gtcggactca
     8161 ctctctcagg ctcgtcagcc tgtgcctgct gtggcgtctc cttaatcact ttgcggcatg
     8221 ccgcactctg ctcgtatcaa tgagagcctg atctcgccgc cgacgccgcc gtgacgcgca
     8281 gagacgagaa aggcgaggca cttttcggtg gcctcgcctt tccctgatct gttcgccccg
     8341 tagagacgat atttgattag tggcggtcgt gacggtgatc gcagtggtgg caattctccg
     8401 ccgcttcctc tgtccagtag acgtgtgtga tcgttttcga accgttcggg ttagacctct
     8461 cgtagatcga actacgcccg ttatgggatt ggctgacgag atgtccccag ctaatcgggt
     8521 cttccgtaat atcagaaccc gcatcttccc ccgcctcatg agccgtggat gtcgtgcgcg
     8581 caccgacggt ggtggaaccg tcgaggtgcg catagatgct ctcaagcaag gcatagtcgt
     8641 gcgagttcgg gcgcgtgctg acggtgctcg tggcgttcgc gccggtgttt gagaagtagt
     8701 ccatgcacgt attctgcgag gtgccgttct cgctcgtgtg gccgaggccg aacgtgtgcc
     8761 cgatctcctg acacatcacg tgtcgtctct cgttgggatt gttgtaggtg gccgtgttga
     8821 agtaggtgtc gttcatcttg gccgaacctt gcgtgatgtg cttcgtgccg ctgaggaggt
     8881 tgatcgaggc gatgcccagc cagccgttgt tgccgtagct gccgttacag acctgtaccg
     8941 tcccgcgcac catcccgcag gccgaacccg ccgagccggt gtatgacgac aggttcagga
     9001 cggaggatgc gctccaatcg gagatcgacc cgctatacgc ggtaccccag gcggaagaaa
     9061 ggtttttccc gaccctgagc gtgaaggggt tcgaggtgcg cgcccagtga tagccgttcc
     9121 acgaatggtt ggcggaagtg acagcggcga aagtggcgat gaccgacaag gccaggacga
     9181 cgagcaggat gcgacgacga gaaattttta acattggagg ctccttattc tttccgtggt
     9241 aggggttgcg ccgccgctca caccagacgg gggggcgcag ttcggagggg cggcggaagg
     9301 atgattcttc acgtcaaccc aggacgaata aagtgtgatg gatactcgtg cgcgagaagt
     9361 ccgcgcacgc atcttcgctt tgagggatga tgccgggtag gctgaagaca gtgaggccgg
     9421 atgtacggtc actaaagtat tatgtgagag ctaaggtaaa gatgaggttg aaactgaaac
     9481 ccgttgatcg aacttgtcgc tgcccgctta cacgaagccc cgcccgaaaa gtataaaaag
     9541 ttaaactgtg ggaaggaaca tctgtgggaa cagagcggcg ggagcatagc agtgaagtat
     9601 gcgcgtcgca acaacattcg tcgcccggcc tggacaggaa caaagtattt actggccgaa
     9661 gttgctatcc cgcttccgca gagggaaatt acgtgcgcgc ggctgctgag taaagcgcga
     9721 ctgctcgctg agaggtgaac tttattcgac ggatttgagc atcaggaggc cgtcaccgcc
     9781 gttggagtag tagcggggga ggcgttgggt gacggtgtag ccgaggttgc ggtagaggtt
     9841 ttgcgcgccg ctgttgacgg agcggacttc gaggcggatg gtgcggacgt ggcggcggcg
     9901 gaagttctct tccaccttga cgagcatctg tgcggcgatg ccgcgccgac ggtgttcggg
     9961 ggcgacgccg agcgtggtga tgtggccggt gttctgcggt tcgacgaggc cgacgatgaa
    10021 gccgaccatg aggccgtcag gcgtgacggc gcgataggcg accgattcgg gcgaggttaa
    10081 aagatattcg aaggtgtcgc ggctgtaggc ttcgccgtcc acgaagcagc gttggtcaag
    10141 tcgccagcat tcgtcgagtt gggagatggt gagcgggcgg atgtcgtaac gcacctcggg
    10201 gagcgaggac gcttgcgtcg cgccctccgt cgcgcgattc tccccctgcg cctcgtcgcc
    10261 gttcgcggac gcttctttcg acggcgcaaa caggcgcgag cgaatttttt tcagcactgc
    10321 cataagaaaa gtgacgagtg acgagtgaca ggtgacaagt ttacactgcg cgcccctcga
    10381 acctgatttt atcttgtcac ccgtcacttg tcactcgtca ctgctttaac tcgtcactcg
    10441 tcactcatcg cttgtcactg cctttaaatg atgagcatgc aatcgccgta gctgtagaaa
    10501 cgatagcgtg cggcgacggc gtggcggtag gctgcgagcg tgagttcgcg tccggcgaag
    10561 gaggcgacaa ggacgagcag cgacgagcgc ggcaggtgga aattggtgac gagcgcatcc
    10621 acgcttctga acctgtagcc gggcgtgatg gtgagcgtcg cgctgcgcgc gcccggcgtg
    10681 aggcgtcccg tcgaatcgtc tgtggcggat tcgagcgcgc gcacggaggt ggtgccgacg
    10741 gcgatgatgc gtcgcccgct tgagcgcgca tcgttgagcg tcgcggcggc ctccggcgtg
    10801 atctcgtaac gctcggcggc caccgcgtgt tcgcttagat cgttcacgcg cacgggcgca
    10861 aacgtgccgt agccgacgtg caaggtgatc tccgtgacgc agactccgcg cgcgcgcaat
    10921 gcttccagca cgcgcggcgt gaaatgaagt ccggcggtcg gggcggctat cgcgccgaga
    10981 cgttcggcgt agatcgtctg gtagcgttcg cggtctgcgg tgcgctgttg cgagagggag
    11041 gatgcgtccg gctcgtccgc gcgtttgatg tagggcggca gcggcacctg cccgatctct
    11101 tcgagcagcg cgtgaaaatc tcccgcgcag tcgaagcgta cggtgcgttg gccgtcgtcg
    11161 cgcgcgtcga taacttctgc gcgcaagcgg cctccgccga aagacaactc ctgaccgcgc
    11221 gccagcctgc gcgccggacg cgcgagcgca tcccacacgg acggctcacg ctctgcgagc
    11281 aggaacagtt cgacccgccc gcccgaaggc tcgcgtcgcc cgacgagtcg cgccggaaag
    11341 acgcgcgtgt tgttgacgac caactggtcg ccctcgcgca actccgcagg cagttgagag
    11401 aaggaactgt cgcgccacga agcgcgcacg cgatccagca cgagcaggcg tgaagcgtcg
    11461 cgctctgcga gcggggcttg cgcgatcaac tcttcgggca gttcgaagtc gaattcggaa
    11521 atcaacataa aaaagagggg aaaggggaag gggtaaaagg gaagggaaac tacgcgcgac
    11581 ttcgttgccg cacgccggtt gtccactcct gactctttcc cacttcccct tccccttgac
    11641 ccttacgaac tatgcctgcg gcatggcggg cgtctgcgtc tcgccgggcg gcacggtgtc
    11701 tgccagttcc tcgtgcgccg tcgagtgctc ctggtgaaag acgacggaga gttgcgcgcc
    11761 gaagagcagg atcaaactcg acacgtaact ccacgtcagc accgcgacga ccgcgcccac
    11821 cgaaccgtaa atctgatcgt agtgaaaata gtgcaggctc gacgcgaaga taaacttcgc
    11881 cgcttcccac aggagtccgg cgacgaccgc gccggggatc gaatcctgca agttcacgcg
    11941 cccgctcggc atgaagcgat agacgagcgt gaagagcgcg accgtcacgg cgatgctgac
    12001 cagcgcgaag acgagttgcc agaaggcgtt gccgacgagc gacaggatcg gcacgcgttg
    12061 cagcacgcgc aggggaagcc gctcggccag ttctttgagc gcgacgagcg tgtaagtgat
    12121 catcacggag gcgatgagca gcacgccgac gccgcccatc atgccgagcg tcaaaccgcg
    12181 cccgtgcagg aaagcgcgcg gcttcgtgcc ccacaggcgg ttgatcgcgc gctccatcac
    12241 gttgaagacc cacgagccag cccagagcgt gacgatgatg caggtgagga tgacgctcgt
    12301 gctgacgttc tccagagagc gcacggtggt gcgcaggaaa tcgcgcgaac cgggataggc
    12361 ttccacggcg cgcgtcagca tctcgctgcc cacgtcgaaa tagttgctca gggcgagcag
    12421 gacgagcagc atcgggaaga gcgtgagcag cccgaagtaa ctcatcgcgg cggcgggcgt
    12481 gaagagatcg ttttcgtgga attgatgaaa cgaatggccg aagatgttca gccacggcgc
    12541 ggcattgcgc acgccgaagc gttggcgggt gaatgagcga agccgtttca taactcaaga
    12601 cggttgaact agacgccgaa tagtccgggg ttcgacggtg tgggactcag cccgaagtgc
    12661 tcgtaggcaa gccgcgtcgc catccgaccg cgcggcgtgc ggttgagaaa gccgatctgg
    12721 atcaggtaag gctctatgat ctcttcgatg gaatctttct cctcgtgcac cgaggcggag
    12781 atcgtgccta gcccgaccgg gccgccgctg aatttttcga tgatcgtcaa caggagtttt
    12841 cgatcaactt cgtccagccc gaacgtgtca acctccatcc ggttgagcgc gtcgtgcgcc
    12901 acttcctgcg tgatgcgccc ctcgtaatcc acctccgcga agtcgcgcac gcgcctgagc
    12961 aggcggttgg cgatgcgcgg tgtgccgcgt gcgcggcgcg cgatctcttt cgcgccgcct
    13021 tcgtcgatct ccgtgccgag gatgtctgcc gagcggcgca cgatgagttc caattcgtcg
    13081 tgcttgtaaa aatcgagatg aaagacgatg ccgaagcgac cgcgcaaggg ggcagtgatg
    13141 agtccggcgc gcgtcgtcgc gccgactaag gtgaacttcg gcaagggcaa cttgatggag
    13201 cgcgccgccg tgccctgccc gatcatgatg tcgagttggt aatcctccat cgccgggtag
    13261 agcacctctt cgatggccgg gttgaggcga tgaatctcgt ctatgaagag cacgtcgcct
    13321 tcctgtaggc cggtgagaag cgcggcgagg tcgcccgcct tttcgatgac gggtccggcg
    13381 gtcgagcgca gttccgcgcc catctcgttc gcgacgatgt tggcgagcgt ggtcttgccg
    13441 agtccgggag gccccgtgag caggacgtga tccagagcct cacggcgcat cgtcgcggcg
    13501 cggatgaaga tgcgcagatt ctccgtcgcc ttgcgctgcc cgatgtactc cgcgagccgc
    13561 gcgggacgca acgacaactc aaactgcgac tcctcatccg tcggcgcaga caagcggacg
    13621 ctggctggtt cgagttcttc agacatacgt taaatgatga acgcgaaacg atgaacgatg
    13681 aatgagagac aactgctttt agtcttcagt tcatcgttca tcgtccatcg tttccggtca
    13741 tcctttcgag agcgatttca ggctgcggcg caggagcagt tcgacggaga gttcgccgcc
    13801 ttcctgaatc gcttgattga tcaccttctc ggccatcgcg cgttggtagc cgaggttggt
    13861 gagcgcggag agcgtgtctt cgcgcagggt gtcctcgctc ggggctgcgc ctgctttttg
    13921 cgcggcgagt tgttcttcga gcgcgggatg cgagagcgtc gtcatcttgt cgcggagttc
    13981 gatgacgagg cgttcggcgg tcttcttgcc gaggccggga atcgaggtca ggcgcgcgag
    14041 attgttggag cgaatggcgc tgatgatctc gtctgcgttc atgccggaga gcatggcgat
    14101 ggccgacttc ggaccaatgc cgctgacgga gattaagagc gtaaagagtt cgcgttcacg
    14161 cgcggttttg aagccgaaaa gttggagggc ttcttcgcgg acgtgcgtgt agatgcgcag
    14221 cgaaatgttc gcccccggct cctccatgtc gtagaaaatg ctgaccggga tggtcacttc
    14281 gtagccgacg ccgccgacat ccaaaatcac actggtggcc tgttttgcga gtaaggttcc
    14341 tgataaatgt gcgatcattc caagttcttt gtttctgccg agagaagaga tttcgggagc
    14401 gtctctgtgt gccgtgagat tgtagttgaa ccgcgaactg acagcaacgc acgagtcttt
    14461 gttttcgctg tgccccaact ctgcgctccg tgccgctatg tcgagatggg aagccgccgc
    14521 cgcagacaca gagaactctt taacctgtca ctcgtcactc gtcacttctc actctctttc
    14581 gttatactga caggcgaggg aagaaaagga aacgggatga agtcggagaa aatttctgaa
    14641 ctgacgacga accgcctcag cgtctacctg cgttgtctga acaaactggc ggctgccgga
    14701 atcaagaccg tctcctcgcg ctcgctcgcg gaggacttca atctcaactc ggcgcagatc
    14761 aggaaagacc tcgcttactt cggcgagttt ggcacgcgcg gcgtcggcta cacggtcaac
    14821 gacctgcgcg cgcacatcac gaagattctc ggactcgaca ccgagcaccg cgtcggcatc
    14881 atcggcgcgg gacatctggg cactgcgctc gccaactaca aaggcttcac cgcctcgaac
    14941 ttcgccgtcg tcgcgctctt cgacaacgac cgtgaacgca tcgggcagac cgtcggcgac
    15001 gcacgcatta cgatctacga tgtgcgccag atcgcgcgcg tggtgcgcgc cgagcagata
    15061 gacgtggccg tgctcgccgt gcccgcacgc gccgcccaga aggttttgaa tcaagtgatg
    15121 tcggcgggcg tcaaggccgt gttgaacttc gcgcccgcgc gcctgcacgc gcgccccggt
    15181 gtgaaattga agacggttga tttgacgatc tcgctggaat cgctctctta tttcctagcc
    15241 cgccccgccg cctccagtac gacgaacgcg cgcaaagttc tctcctttga agaactcctg
    15301 caacccgaag agaacgagct ttagcgcggc gcgcaataag gagtcgccat gagcgaagac
    15361 ttgacccaaa acttacccga cacattccag gaacgcgtgc ttgcggaact aacagctatc
    15421 aggcaagaga acgcgcggtt gagcgttcaa gtgatctcga ttgatacccg tctaacttcc
    15481 cttgaagaca aagttgatgc gcggctgcgt gagacgcgcc cgatttggga gggcgtgctg
    15541 gagcgtctga cggagatcga aaagggactg agcacgctca accgccagtt caagacgatg
    15601 atcaggggag agttcgacct gcacacgcgg gtcgagaagt tggaggatga tcagcaggcg
    15661 gtgtgaggtg attttatgaa gtgcgcgttg acaaaaattt tgccgttcat cctcacgctg
    15721 ttcgtcggcc tgacgctcgg caacttctca gggcgacccg cgctgcatcg agttcgggac
    15781 cgggggcagg cgatgcaggg caatgttcag caggggcgga cgtggctcg
//
LOCUS       DASWNS010000242        12505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_478354, whole genome shotgun sequence.
ACCESSION   DASWNS010000242 DASWNS010000000
VERSION     DASWNS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478354"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VGB76_08395"
     CDS             complement(<1..1191)
                     /locus_tag="VGB76_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0098961.1"
                     /translation="MTEETSPRRTIVCLASYFKGVEFLRECKRQNCRVFLLTKEKWRN
                     EAWPHDSIDELVTLPNDASPDHFIYAATQIARHHKLARVVALEEFDVITAGLIREHLR
                     LGGMTSAEARLFRDKLAMRGAAQAAHVNVPDFVHVLNYAELSDFMERVPAPWVLKPRT
                     DVSAIGIRKVHDAEQVWRAIDELDARPRLNEQSSFYLLERYVAGEVYHVDSIVSGGRV
                     SFAGVSQYGRPPMNVAHEGGVFISYSVERKNDDRRKLLEINRQVIKALGLRRGATHAE
                     FIKSRDDGKFYFLEIASRVGGAYIAEALEAACGLNIWREWAKLELSDDEHPYRAEPTR
                     EEYGGIVLSLARQERPDTSNYIDTEIVYRIRKPYHVGLVVRSPDHARVLGLLDEYARR
                     FADEF"
     gene            complement(2001..3428)
                     /locus_tag="VGB76_08400"
     CDS             complement(2001..3428)
                     /locus_tag="VGB76_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0098962.1"
                     /translation="MSEKKTTAQYSPGAHRRDDQRELDASNGRLDESPACESSVPAAD
                     EDLKVLLDSWQSPLVVVPASLDARVLNSYRELLKEKEMNLQQRPGDLLAPQYALSSAA
                     GALAPAEYRLTILEERGLFKRLLAEMRETAHDAELTRAEFRRNPFRFVGRMSVAYGAM
                     TWRTLRQENTAYGVVAALVAVVLLFGAVAALDGFQQRQGGLLAERLREDLVYLGSVTE
                     IPKEQPKPEEGTAGMAKGTGGGSKPEPERAGGGGGGGRLEDKPAVNGKLPQATLQPQV
                     LAPDPHPPTIRNPSLPTAATIQVDPALFPTDTRAINYGDPKSKATELSSGSGEGNGIG
                     DGTGGGVGSGRGTGYGPGEGFNTGGGPAKPGGGGAGGNGGTPDYARPFNPKEVTRRAN
                     ITSKPEPLYTEEARKNQVTGTVRLRLILSASGQVTGITPLTKLPDGLTEKAIEAARRI
                     SFTPAEKDGRKVSQYVTIEYNFNIY"
     gene            complement(3425..4024)
                     /locus_tag="VGB76_08405"
     CDS             complement(3425..4024)
                     /locus_tag="VGB76_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0098963.1"
                     /translation="MPDTDTDEELLRRARAGEEDAFLSIYTRHRVAMYRFACRLLRSE
                     SAAEDVTHDCFLSLFKNPERFDPSRGTSLRTYLFAAVHNLAMKQFRRAGQEAWMEEAT
                     LTEPRTPDTDEPLSRLIEAELSAAVRAAVESLPALQREVLILFEFEDLSLAEIAVVVD
                     AEVNTVKSRLSRARAGLRRTLAPYLERREELVGASERTL"
     gene            4440..5420
                     /gene="selD"
                     /locus_tag="VGB76_08410"
                     /pseudo
     CDS             4440..5420
                     /gene="selD"
                     /locus_tag="VGB76_08410"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            5433..5813
                     /locus_tag="VGB76_08415"
     CDS             5433..5813
                     /locus_tag="VGB76_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098964.1"
                     /translation="MIGDEMERTMQFILEQQAQFMANIQILQEQRARDEGRLARVERA
                     FVQLTEVAVNANERMNEQEARHTELHARDTERLARLERAFVQLTELAVSANERMNEHD
                     TRLERVEEAVVLLTRLVGGKEGDG"
     gene            5828..6691
                     /locus_tag="VGB76_08420"
     CDS             5828..6691
                     /locus_tag="VGB76_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0098965.1"
                     /translation="MLNTDERFESGAPAYAAYLQTSEGRLRLDIAWENFLSLVNETNA
                     AQAKFDERGARAETSGRALDLGGGTGALGLRFAALGWRVAIVDPSATMLSLAAEAAQR
                     QGLDGRVTFHQAVAERARECFPPQTFDAATCHNVLEYVSDPRAALVALAGLVRAGGLV
                     SLVARNRAGEAMRAALKAHDLDEAEHALSTDTVNESLYGGPARLFDREMLCELATGAG
                     LEVIAARGVRVVADYLPASLSDTPETYARLLAFERTLGARPEFAAVARYTQVIARRPP
                     GTATASGRGSS"
     gene            6709..7560
                     /locus_tag="VGB76_08425"
     CDS             6709..7560
                     /locus_tag="VGB76_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0098966.1"
                     /translation="MNDNENPDRASSDRVMTKRARVDRRPWSVFLKIFICAAASLTLL
                     GGSLGSALQHQVASNRSPDTLHAISESPELPSQQSKAAQTPARDADKLDRPTSEPYKG
                     SLSIFEDAKREEKLQINRVMDILGIREGSRVADIGAGSGWFTVCAAQRVGARGVVYAV
                     EINEEYLKHIAERASKEELSNIRTILGKEDDPLLPKASVDAVLILKTYHEIAEPIRLM
                     RALRRSLAAGALVGIIDRNGAGDDHGLDDRKVIREMERANFALVAQYDFVKPDGMDYF
                     LVFRARA"
     gene            complement(7557..9440)
                     /locus_tag="VGB76_08430"
     CDS             complement(7557..9440)
                     /locus_tag="VGB76_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098967.1"
                     /translation="MKYTKSLAALLLTFTLSPNAIVAQQPFAAAAAPPVAKAEEPTAE
                     QLELERKALVLLNEIIGETPSLKLVENRIRLQAVEAELLWPRNQERAREIFNAAAVDL
                     AAMTASIEPDDQQYYSLVNSASQMRQRMLNTIAQYDPKLALEFLRSTRTPPPMAQPGG
                     NFRPPDQEAMLESQLAQQIAARDPQQALRLAEEILSRGLSANLLPLLDLLRTRDPEGA
                     TRLTASLVKKLRTANFATDREAVNIANFLLHSTRGAENSNANSQAASGATTIPANLPN
                     PRRLQLDEATRRELVSSLVNAAVNTPNSGGRSNNAGYLLSTVQQLMPEVERYAPTQAA
                     GLRRRLENSERRAEPGVNQRELGQLMQSGTTDALIEAAAKASPEMRPQLYRAAAWKAF
                     NEGSPERARQIINTHVEGGRQREQLLRELDEQLFGRAVSEGNVEGAQALLARAKSTED
                     RVGMSILLARALLDKGNQKGALGFLDDAWMLVQGRAKGQLQFNQQLVLAQVYARLAPA
                     RAFEIVEASITHLNELVGAAAVLDGFGQEAFAQDELKVRDGHVWNALVAQCGETLAAL
                     APVDFEHARSAAERFQRPELRLQARLAVVRGILVKESEQDSFNRLRRGGRRPNPARII
                     RQD"
     gene            complement(9480..10211)
                     /locus_tag="VGB76_08435"
     CDS             complement(9480..10211)
                     /locus_tag="VGB76_08435"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0098968.1"
                     /translation="MKDEPEVNTRFNSDARVSVVIPALNEAETIGAVVRAVPREIASE
                     VIVVDNGSTDATAERAREAGARVVREARRGYGRACRAGVEAVARECEIVVFLDGDGSD
                     CPELMNRLVAPIKAGTHDFVIGSRTRGQREQGSMNFQQRVAGRGAGLLLGALYGVNYT
                     DMCPFRAIRRDALRSLPMREETYGWNLEMQMRAARARLRILEVPVDHRRRAGGESKVS
                     GTVRGTVLAGTRILYTVARIAWERT"
     gene            complement(10326..11468)
                     /locus_tag="VGB76_08440"
     CDS             complement(10326..11468)
                     /locus_tag="VGB76_08440"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0098969.1"
                     /translation="MTRTSNNGRRALVTGGAGLIGSHLVDLLLREGYSVRILDNLEPN
                     THRAGRPAWINPQAEFVEGDIRERAVIAAALAGVDVVFHQAAYGGYMPEISKYVSVNS
                     FGTAQMLEVIRDENLPVKKIVVASSQAVYAEGAGRCVEHGLVFPAVRPVGQLRAGDFS
                     VHCPQCDRPTTLAPTPERAPIGGETVYAITKVDQERLVLTWGQQTGTPTVALRYSCTY
                     GPRQSIFNPYTGVIAIFSTRLLNRQPPVLYEDGEQTRDFSFVEDIARANLLAATTDKL
                     DGLPVNVGSGEATTIREIARLVADALNIALEPLAHGEFRPGEMRHLISDTTRIRAAGY
                     APQVKLADGIRRYIEWIGQQMDVRDYFAAAEQLLRAKGIVHRVAAK"
     gene            complement(11528..>12505)
                     /locus_tag="VGB76_08445"
     CDS             complement(11528..>12505)
                     /locus_tag="VGB76_08445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY0098970.1"
                     /translation="DALRFLRDEAIYPNINRRDFAPTIYPPLAQLIFLLATRVSESVV
                     WMKAVMVLFEVAGLCALAALLAAFKLPRERVLLAAWHPLVVWEIAGNGHLDALVVAFV
                     AFALVAHRRGRESLTGALLAGATLVKLFPLVLLPAFYRRWSWRLPLVFCAVFAAFYLP
                     YLSVGVRRVAGYLPGYAEEEGLQSGARFFLLQLARSFPGNSGIPRAVYIVFALLVLGA
                     IAAWCVYARAPDARDFVRRRAPLLAVVFTVLLSPHYSWYFVWLVPFLCFVPAARVAPL
                     LYLTAASFMLYGTWLGDAPRQMLWLNSCIYLPPAFLLLVFRVRPEHKDV"
BASE COUNT         2260 a         3893 c         3978 g         2374 t
ORIGIN      
        1 gaactcgtcc gcgaagcggc gcgcgtattc gtcgagcagg ccgagcacgc gcgcgtggtc
       61 gggcgaacgc acgacgaggc cgacgtgata aggcttgcgg atgcgataga cgatctccgt
      121 gtctatgtag tttgaagtgt ccggcctctc ctgccgcgcg agcgagagca cgatgccgcc
      181 atactcttcg cgcgtgggtt ctgcccggta aggatgctcg tcgtcgctca gttcgagttt
      241 cgcccactcg cgccagatgt tgaggccgca cgccgcctcc aacgcctctg cgatgtacgc
      301 gccgccgacg cgcgaagcga tttcgagaaa atagaacttg ccgtcatccc tgctcttgat
      361 gaactcggcg tgcgtcgccc cccgcctcaa tccgagagct ttgatcacct gacggttgat
      421 ctcaaggagc ttgcgccggt cgtcattttt tcgttcgact gaataggaga tgaagacgcc
      481 gccttcgtgc gcgacgttca tcggcgggcg gccatactgg ctgacccctg caaacgacac
      541 tctgccgccg gagacaatcg agtctacgtg atagacctcg cccgccacgt atcgttcgag
      601 caggtagaag gaggactgtt cgttcaggcg cgggcgcgcg tcaagctcat caatcgcgcg
      661 ccagacctgt tcggcgtcgt gcaccttcct gatgccgatg gccgagacat ccgtgcgcgg
      721 cttcaagacc cacggggcgg gcacgcgctc catgaaatcg ctcaactccg catagttgag
      781 cacgtgcacg aagtccggca cgttgacgtg tgcggcctgc gccgcgccgc gcatggcgag
      841 cttgtcgcga aacaggcgcg cctccgcact cgtcatgccg ccgagccgca ggtgttcacg
      901 aatcagcccc gccgtgatca cgtcgaactc ttcgagcgcg acgacgcgtg cgagtttgtg
      961 atggcgcgcg atctgcgtgg ccgcgtagat gaagtggtcg ggcgaggcgt cgttcggcag
     1021 cgtgacgagt tcgtcaatcg agtcgtgcgg ccatgcttcg ttccgccact tctctttggt
     1081 gagcaggaag acgcggcagt tctgccgctt gcattcgcgc aggaactcga cgcccttgaa
     1141 gtagctggcg agacagacaa tggttcgtcg gggagatgtt tcttcggtca caggcgactc
     1201 tctcgttgat gtgtcggcac gaatcaagct gcggggtttg acgcgcctca ctggaatttt
     1261 cgtggagcag tatataacgg gcgcgcgccg ggagacacca gccgccggtc tgcttttgca
     1321 agggaaagct cccgccgcag ggagacgctt gaaccggcgt tgcttgagtt ggttgatgaa
     1381 gtgtccggtc gcgcgccgcg cgcttacctg aaaatcttga ctttcgggtt tccggggcgt
     1441 agggttcagc ctccactcta aaacctcact tccacgaaac agattaccgg gtgggaaagt
     1501 ttttgcctga gacggaggcg gggcttactt cagcaaaccc taatacggaa ggatgaagaa
     1561 ttatgaagca gtttgtgtct tcgaaacttc tcaggaaagt gttaacgctc gctttcctta
     1621 tcgcggctct ggtgtttatc gtctccggcg acaaaatacc ccaacccgtc tacgcggctc
     1681 catgctgtga agaatgtgac ggcgcggagc ttgatgcctg catggcggaa tgcacgacct
     1741 ctgcttgcag aagaaattgt attaacaatt cctgtgaagc gaactgcgtc tcgtgcggcg
     1801 gaggcggcgg tggcggatgt ttgaccaacg ccgattgtcc atatttaccc ggatatggat
     1861 ttgccaattg tgtgggcggt aattgtgtct attagcagtt cggacatcta accgggagaa
     1921 gctctcaact acccgaaaaa agcaggcggc gactcatgtg tttatgagtc gccgcccttg
     1981 tctgtgcgcc tgaatcgaat tcagtaaatg ttgaagttgt attcgatggt gacgtactgt
     2041 gagactttgc gcccgtcctt ctcggcaggc gtgaaagaga tgcgccgcgc cgcttcaatc
     2101 gccttctccg tcaggccgtc gggcaacttc gtcagaggcg tgatgcccgt tacctgtccc
     2161 gaagcggata agatgagacg caggcggact gtgcccgtga cctgattctt gcgcgcctct
     2221 tccgtgtaga gcggttccgg cttgctcgtg atgttcgccc tgcgcgtcac ttccttcggg
     2281 ttgaacgggc gcgcgtaatc gggggttccg ccgttgccgc ccgctccgcc gcctccgggt
     2341 ttcgccggac caccgcccgt gttgaagcct tcgcccggtc cgtagccggt gccccgccct
     2401 gaaccgactc cgccgcccgt gccgtcgccg atgccgttgc cttcgcccga acccgaagag
     2461 agttcggtcg ctttcgattt cgggtcgccg tagttgatgg cgcgcgtgtc cgtgggaaac
     2521 agggcggggt cgacctgaat cgtcgccgcc gtggggagcg aagggttgcg aatggtgggc
     2581 gggtgcgggt ctggcgcgag cacctgcggc tgcaaggtcg cctgcgggag tttgccgttg
     2641 acagccggtt tgtcttcaag ccgaccgccg ccgccaccgc cgcccgcgcg ttccggttcc
     2701 ggcttcgagc cgccgcccgt gcccttcgcc atgcccgccg tgccttcctc cggcttcggc
     2761 tgctctttgg gaatctccgt tacgctgccc agatagacca gatcttcgcg gaggcgttcg
     2821 gccaacaagc ctccctgccg ctgctggaaa ccatcgagcg cggcgaccgc gccgaacagc
     2881 aggacgacgg cgacgagagc ggcgacgacg ccgtaagcag tgttctcctg ccgcagcgtg
     2941 cgccacgtca tcgcgccgta ggccacgctc atgcgtccta cgaaacggaa ggggttgcgg
     3001 cgaaactccg cgcgcgtgag ttcggcgtcg tgcgccgtct cgcgcatctc cgcgagcagg
     3061 cgtttgaaga gtccgcgctc ctctaagatg gtgagccggt attcggcggg cgcgagtgcg
     3121 cctgcggcag aggacagagc gtattgcggc gcgagcagat cgcccgggcg ttgttgcaga
     3181 ttcatttctt tctccttcag cagttcgcgg taagaattaa ggacgcgcgc atcgagcgat
     3241 gcgggcacaa ccacaagggg agactgccaa ctgtcgagca aaactttcaa atcttcatcc
     3301 gccgccggca cagacgactc acacgcaggc gactcatcca gacgcccgtt cgacgcgtcg
     3361 agttcgcgtt gatcatcgcg gcggtgcgcg cccggagaat attgtgctgt cgtcttcttc
     3421 tcgctcataa cgttctctcc gacgcgccga ccaactcctc gcgccgttcg agataaggag
     3481 ccagcgtgcg ccgcaatccc gcgcgtgcac gcgacaggcg cgacttcacc gtgttgacct
     3541 ccgcatccac caccacggcg atctccgcca gactcaaatc ttcaaactcg aacaggatca
     3601 acacctcgcg ctgcaacgcc gggagactct ccaccgccgc gcgcaccgcc gccgacaact
     3661 cggcttcaat caagcggctc agaggttcgt ccgtgtccgg cgtgcgcggc tccgtgagcg
     3721 tcgcctcctc catccaagcc tcttgtcccg cgcgccgaaa ctgcttcatc gcgagattgt
     3781 gcacggcggc gaaaaggtag gttcgcagag acgtgccgcg cgaagggtcg aagcgttcgg
     3841 gatttttgaa caggctcagg aagcaatcgt gcgtcacatc ttcggcggcg gattcggagc
     3901 ggagcaggcg gcaggcgaag cgatacatgg cgacgcgatg gcgcgtgtag atcgagagaa
     3961 acgcgtcctc ttcgcctgcg cgtgcacgcc ggagtagttc ttcgtctgtg tctgtatcag
     4021 gcaaaacctg agatgctcgt ttccgtcttc gagagcttgc tgccgtctgt tgttaccgcg
     4081 cgggcgcgaa aggatcgggg cagatgaaaa aaaagatgaa ggatgaggga tgagggatga
     4141 gggatgagta agaaactact caacgccatg agtgcttttc ttattcatcc ctcatccctc
     4201 atccttcatc ccttctctat gctgtcgctt tgttgcacga cgccgcgcgg gcggttgctc
     4261 acacgtctcc gtcaattttt tagaacgtga ttttttttcg cttgagagcg acgccggttc
     4321 acgcacgcga ttttggtggc gcgtgctcca actgttaatc taagtcgcag tttcaaagaa
     4381 gggagcacaa gatgcgtctg acggagatgg tcaactgcgc cggttgagcg gcgaaactcg
     4441 cgcccggcga actcgctcag gtcttgagcg ggctgcacat tccgaaacat cccaatctgc
     4501 tggtcggcat cgagacggcg gacgacgccg gagtctatct cctgaacgaa gacctcgcgc
     4561 tcgtccagac cgtagacttc ttcacgccca tcgttgacga cccctacgac tacgggcgca
     4621 tcagcgcgct caactcgatc aacgacgtgt gggcgatggg cggcacgccc atcaccgcgc
     4681 tcgccgtcac ctgcttcccg aagaagggcg tcgatttcaa gatactcggc gagatcatgc
     4741 gcggcggttt ggacgtgctg accgcgcacg gcgtcgcgct cgtcggcggt cacagcgtag
     4801 acgatccgca gatcatgttc ggctacgccg tgacgggcac gatcaacccg cgccagatca
     4861 tcaccaatcg cggcgcacgc gcgggcgacg cgctcatcct gacgaagccc atcggcacgg
     4921 gcgtcatctc cacgggcatc aagttcggca aggcgggcga tgaagtgatg ggcgcgtcgc
     4981 tccgcacgat gttgctcgct gggcgcgagg ccgcccaact gatgcgcgag ttcggcgcgc
     5041 acgccgcgac ggacgtaaca ggctttgcgc tcctcggcca cgcgtgggag atggcgcgcg
     5101 gcagcaacgt cacgcttgag atcgaagcgg cgcgcgtgcc gctcatcccc ggcgcgctcg
     5161 aactcgccgc gcagggacta ctcacgagcg gcgacaaaac caatcgcgct tacgtcggcg
     5221 acgacatcga gatcgccgcc ggagtgtcga agccgcacgc aagtctcctc ttcgatccgc
     5281 agacggcggg cggccttctc atctcgattg aagagtcgcg cgccgatgcc ctgctcgcac
     5341 gcctcaacga aacctaccct gacgcctctc gtatcggtcg catcgcggaa cgcggcgcgt
     5401 attctctgtt agtcaaatga ggagaaacga aaatgatcgg cgatgagatg gaaagaacga
     5461 tgcaattcat tcttgagcag caggctcagt tcatggcgaa cattcaaata cttcaagaac
     5521 agcgcgcccg cgatgaggga cgcctggcgc gtgttgagcg tgctttcgtt caactcacag
     5581 aggtggccgt gaatgccaat gaacgcatga acgagcagga ggcgcgtcat actgaacttc
     5641 atgcgcgcga tactgaacgg ctggcacggt tggagcgtgc tttcgttcaa ctaactgagt
     5701 tagcagtgag tgcgaacgag cgcatgaacg aacacgatac gcggctggag cgcgtcgagg
     5761 aagcggtcgt gctgctgacg cgtttggtcg gcggcaaaga gggcgatgga taggtgaagt
     5821 gcgggagatg ctgaacacgg acgaacgctt cgagtcgggg gcacccgctt acgccgccta
     5881 cttgcagacg agcgaggggc ggctgcgtct cgacatcgca tgggaaaatt ttctttcact
     5941 cgtcaacgag acgaacgcgg cacaggctaa attcgatgag cggggagcgc gcgcagaaac
     6001 gtcgggacgc gcgctcgatc tcggcggcgg cacgggtgcg ctcggcctgc gcttcgccgc
     6061 gctcggctgg cgcgtcgcca tagttgatcc gtcggcgacg atgctctcgc tcgcggcaga
     6121 ggccgcgcag cggcagggac tcgacgggcg cgtcacgttt catcaagccg tcgcggagcg
     6181 tgcgcgcgaa tgtttcccgc cgcagacgtt cgacgcggcc acctgtcata acgtgctcga
     6241 atacgtgtcc gatccgcgcg ccgctctcgt cgcgctcgcc gggttagtcc gtgcgggcgg
     6301 attagtctcg ctcgtcgcgc gcaatcgcgc gggtgaagcg atgcgcgcgg ccttgaaggc
     6361 gcacgatctg gacgaagcgg aacacgcgct ctcaaccgac acggtgaacg aatcgctcta
     6421 cggtggtccc gccagactgt tcgaccggga gatgctctgt gagttggcga ccggggcggg
     6481 acttgaagtg atcgctgcgc gcggcgtgcg cgtcgtcgcg gactacctgc ccgcttcgct
     6541 ttccgatacg ccggagactt atgcgcgcct gctcgccttc gaacgcacgc tcggcgcgcg
     6601 tcccgaattt gccgccgtcg cgcgctacac gcaggtcatc gcacgccgcc cgcccggcac
     6661 ggcaaccgca tcaggtcgcg gctcatcgta aacaagatgc ttgatgagat gaatgataac
     6721 gaaaatccgg atcgcgcgag ttcggatcgt gtcatgacga agcgtgcgcg agttgatcgc
     6781 aggccgtggt ctgtgttcct gaaaatcttc atctgtgcgg cggcgagtct gacgctgctc
     6841 ggcggctcgc tcggttcggc tctgcaacat caggtcgcat ccaaccgaag cccggacacg
     6901 ctccacgcca tttccgagtc tcccgaacta ccttcgcaac aatccaaagc cgcgcaaact
     6961 ccggcgcgcg acgctgacaa actcgaccgc ccgacgagcg agccttacaa gggcagcctc
     7021 tcgatcttcg aagacgcgaa gcgcgaagag aaacttcaga tcaaccgcgt gatggacata
     7081 ctggggatca gggaaggctc gcgcgtggcg gacatcggcg cgggttcggg ttggttcacg
     7141 gtgtgcgcgg cgcaacgggt cggcgcgcgg ggcgtggtct acgctgtcga gatcaacgag
     7201 gaatatctca agcacatcgc ggagcgcgcg tcgaaagaag aactgtcgaa cattcgcacc
     7261 atactcggca aagaggacga tccgctcctg cccaaagcga gcgtcgatgc cgtgctcatc
     7321 ttaaaaactt atcacgagat cgccgaaccg attcgcctga tgcgcgccct gcgtcgttcg
     7381 ctcgcggcgg gcgcgctcgt cggcatcatc gaccgcaacg gcgcgggcga cgatcacggg
     7441 ttggacgacc gcaaagtgat acgggagatg gagcgtgcga acttcgcgct cgtcgcacaa
     7501 tacgacttcg tgaagcccga cgggatggat tactttctag tctttcgcgc acgggcttag
     7561 tcttgcctga tgattcgcgc agggttgggt cgcctgccgc cgcgacgcag gcggttgaaa
     7621 ctatcctgct cgctctcctt cactagaatt ccgcgcacga cggcgagccg cgcttgcagg
     7681 cgcagttcgg gtcgttgaaa cctctctgcg gcggagcgcg cgtgctcgaa gtctacgggc
     7741 gcgagcgcgg ccagcgtttc gccgcattgc gcgacgagcg cgttccagac atgaccatcc
     7801 cgcactttga gttcatcttg cgcgaaagcc tcctgtccga agccgtcgag gacggctgcc
     7861 gccccgacga gttcgttgag atgcgtgatg ctcgcctcga cgatctcgaa ggcgcgcgcc
     7921 ggggcgagcc gcgcatagac ctgcgccagc accaactgct ggttgaactg caactggcct
     7981 ttggcgcgcc cctgaacgag catccaggca tcatccagaa aaccgagcgc gcccttctga
     8041 ttgcctttgt ccaacagggc gcgcgccagc aggatcgaca tccccacgcg atcctccgtt
     8101 gacttggcgc gcgccagcaa cgcttgcgct ccttcgacgt tgccctcgga gacggcgcgc
     8161 ccgaaaagtt gttcgtccag ttcgcgcaaa agctgttcac gctgccgccc gccttcgacg
     8221 tgtgtgttga tgatctggcg tgcgcgttcc gggctgcctt cgttgaaggc tttccaggcc
     8281 gccgcgcggt agagttgcgg gcgcatctcc ggcgaggctt tggccgctgc ttcgatgagc
     8341 gcgtccgtcg tgcccgactg catcaactgg cctagttccc gctggttgac gcccggttcg
     8401 gctctccgtt cggagttctc cagacggcga cgcaagcccg ccgcctgcgt gggggcgtag
     8461 cgttccactt ccggcatcaa ctgctgcacg gtcgaaagca ggtagcccgc gttgttcgac
     8521 cgcccgccgc tgttgggagt gttgacggcg gcgttgacga gcgaactgac gagttcgcga
     8581 cgcgtcgctt cgtccaactg caagcgacgc gggttgggca ggttggcggg aatcgtcgtt
     8641 gcgccgctgg ccgcctgaga gttcgcgttt gagttctccg cgccgcgtgt cgagtgcaga
     8701 agaaagttgg cgatgttgac ggcttcgcgg tcggtggcga agtttgcggt gcgcagtttt
     8761 ttgaccagcg aggcggtgag gcgcgtcgcg ccttcggggt cgcgcgtgcg caatagatcg
     8821 agcagcggca gcagattcgc agagaggccg cgactcaaaa tctcttcggc gaggcgcagg
     8881 gcttgctgcg ggtcgcgcgc agcgatctgc tgcgcgagtt gggattccaa catcgcttcc
     8941 tgatccggcg gtcgaaagtt gccgccgggt tgagccatgg gcggcggcgt gcgcgtcgag
     9001 cgcaggaatt cgagcgcgag tttcggatcg tattgcgcga tggtgttgag catccgctgg
     9061 cgcatctgcg aggccgagtt gacgagacta taatactgct ggtcgtccgg ctcgatgctc
     9121 gccgtcatgg ctgcgagatc gacggcggct gcattgaaga tctcgcgtgc gcgctcctga
     9181 ttgcgcggcc agagaagttc agcctcgacg gcctgcaagc ggatgcggtt ctcgacgagt
     9241 ttcagagaag gcgtctcgcc gatgatctcg ttgagcagga cgagagcctt ccgctcaagt
     9301 tccaactgtt ccgccgtcgg ctcctccgct ttggcgacgg gcggcgcagc ggcggcggcg
     9361 aacggctgtt gcgcgacaat tgcgtttgga gagagcgtga aagtgagaag cagcgcggcg
     9421 agtgattttg tatatttcat aaaagtcttg agtggttgag cgtcgggcgt gcggggagtt
     9481 caggtgcgct cccatgcgat gcgcgcgacg gtgtaaagga tgcgcgttcc ggccagcacg
     9541 gtgccccgca ccgtgcccga aactttcgac tcgccccccg cccgccgacg gtgatcgacc
     9601 ggcacttcca gtatgcgcag gcgcgcacgc gcggcgcgca tctgcatctc cagattccaa
     9661 ccgtaagtct cctcgcgcat cggcagggag cggagcgcgt cgcggcggat cgcgcgaaac
     9721 gggcacatgt ccgtgtagtt cacgccgtag agcgcgccga gcagcagccc cgccccgcgt
     9781 cccgccacgc gttgttgaaa attcatgctg ccctgttctc tttgcccgcg cgtgcgcgag
     9841 ccgatcacga aatcgtgcgt gcccgccttg atgggggcaa ccagacgatt catcaactcc
     9901 gggcaatcgc tcccgtcgcc gtccagaaag acgacgatct cgcactccct tgcgacggcc
     9961 tccacgcccg cgcggcacgc ccgcccgtaa cctctgcgcg cctccctcac gacgcgcgct
    10021 ccggcctcgc gcgcacgctc cgcagtggcg tccgtgctcc cattatcaac cacgatcacc
    10081 tccgacgcga tctcgcgcgg cacggcgcgc acaaccgccc caatcgtctc cgcttcgttc
    10141 agagcgggga tgacgacgga gacgcgcgcg tcgctgttga atcgggtgtt cacctccggc
    10201 tcatccttca tccttcatcc ttcatccttc atccttcgtc gccgaccgct gcgccttctc
    10261 tattgatcgc tgacggttgc gccttctccg cgtcctccat gtctccagcg gtgcgcgcgg
    10321 catctctatt tcgccgccac gcgatgcacg atgcctttgg cgcggagcaa ttgctcggct
    10381 gccgcgaagt agtcgcgcac gtccatctgt tgcccgatcc attcgatgta gcggcggatg
    10441 ccgtcggcga gcttgacctg cggggcgtag cctgcggcac ggatgcgcgt cgtgtcggag
    10501 atgagatggc gcatctcgcc ggggcgaaac tctccgtgcg cgagaggttc aagcgcgatg
    10561 ttcaacgcgt ctgcgacgag ccgcgcgatc tcgcggatgg tggttgcctc cccgcttccg
    10621 acgtttacgg gcaggccgtc gagtttatcc gtcgtcgcgg cgaggaggtt ggcgcgggcg
    10681 atgtcttcga cgaagctgaa gtcgcgcgtc tgctcgccgt cttcgtagag cacgggcggc
    10741 tgccggttca ggaggcgcgt cgagaagatg gcgatgacgc ccgtgtacgg gttgaagata
    10801 gactggcgtg gtccataggt gcaggagtag cgcagggcga cggtcggcgt gcccgtctgt
    10861 tgcccccacg tcagcacgag ccgctcctga tcgactttcg tgatggcata gaccgtctcg
    10921 ccgccgatgg gcgcgcgttc gggcgtcggc gcgagagtag tcgggcggtc gcactgcggg
    10981 caatggacgc tgaagtctcc ggcgcgcaat tgtccgacgg gtcgcactgc gggaaagacc
    11041 agaccgtgct cgacgcatcg accggcccct tccgcgtaga ccgcttgcga cgaagcgacg
    11101 acgatctttt tgaccggcaa attttcgtcg cggatcactt cgagcatctg cgccgtgccg
    11161 aaactgttga cgctcacgta ctttgaaatc tccggcatgt agccgccgta agccgcctga
    11221 tgaaagacga catcgacgcc cgcgagcgcg gcggcaatca cagcgcgttc acggatgtcg
    11281 ccttcgacaa actcggcttg cgggttgatc cacgcgggtc gccctgcccg gtgcgtgttc
    11341 ggctcaaggt tatcgaggat gcgcacgctg tagccttccc gcagcagcag atcgacgagg
    11401 tgcgagccga tcagtcctgc gccgcccgtc acgagcgcgc gtcgtccgtt gtttgaagtt
    11461 cttgtcaact ctcttctcgc ctccacatgt ttcagccgaa cttgtccggt tcgtgttctt
    11521 caaagtctca aacgtctttg tgctccggtc gcacgcgaaa gacgaggagc aggaaggcgg
    11581 gcggcaggta gatgcacgag ttgagccaga gcatctgccg gggcgcgtcg ccgagccatg
    11641 tgccgtagag catgaaggag gccgccgtca ggtagagcag cggcgcgaca cgcgcggcgg
    11701 gcacgaagca caggaaaggc acgagccata cgaaatacca actatagtgc ggcgacaaca
    11761 gcacggtaaa gacgacggcc agtaacggcg cacgacgccg cacgaagtct ctcgcatccg
    11821 gcgcgcgcgc gtagacgcac cacgcggcta tcgcgcccag cacaagtaag gcgaagacga
    11881 tatacacggc gcgggggatg cctgagttgc cggggaaact tcgcgcgagt tgaagcaaaa
    11941 aaaagcgcgc gccgctctgc aagccttcct cttcggcgta gcccggcagg taaccggcga
    12001 cgcgccgcac gccgaccgac aggtaaggca ggtagaacgc cgcgaacaca gcgcagaaca
    12061 cgagcggcag acgccaactc caacgccggt agaacgctgg aagcaggacg agcggaaaca
    12121 gtttaacgag cgttgccccg gctaggagcg cgcccgtcaa actctcgcgc cctctgcgat
    12181 gtgcgacgag cgcgaacgcg acgaaggcga cgacgagcgc atcgagatgc ccgttcccag
    12241 cgatctccca gacgacgagc ggatgccacg cggcgaggag cacgcgctcg cgcggcaact
    12301 tgaacgcggc gagcagcgcg gcgagcgcgc acaagcccgc aacctcgaac aagaccatga
    12361 cagccttcat ccagactacg gactcgctca cgcgcgtcgc cagcaggaag atcaactgcg
    12421 cgagcggcgg gtagatggtc ggcgcaaagt ctctgcgatt gatgttcgga tagatcgctt
    12481 cgtcgcgcaa aaacctgagc gcatc
//
LOCUS       DASWNS010000243        25439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_193074, whole genome shotgun sequence.
ACCESSION   DASWNS010000243 DASWNS010000000
VERSION     DASWNS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 25439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25439
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193074"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..574)
                     /locus_tag="VGB76_08450"
     CDS             complement(53..574)
                     /locus_tag="VGB76_08450"
                     /inference="COORDINATES: protein motif:HMM:NF042765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6702 family protein"
                     /protein_id="HEY0098971.1"
                     /translation="MKFNVYMLSLALVACALTSPRIVPATAAAHKFHTSFAEADYNAQ
                     EASLQITLRTFPDDLEMILGKRSGKSVRLDRKQEAEPLLIAYLQETFQIKNAKGETLK
                     LSWVGLDAGVDSAWLYFEARMPDGFAGAQLRNQFLFDLYDDQVNLVNVKQENRKRALT
                     FKNGDTFQTLAPQ"
     gene            complement(707..3052)
                     /locus_tag="VGB76_08455"
     CDS             complement(707..3052)
                     /locus_tag="VGB76_08455"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY0098972.1"
                     /translation="MLTKIMRSFVFAVLCCLLAPAHAPAQSKTKPSDKFKQLEEILPT
                     PNEQRAASGAPGRSYWQQRADYNINVELDDANQRIIASETITYHNQSPDALPYLWLQL
                     DQNIWKQDSDARLTSTAPNFERVPLNQIENLLYTRSFEGGYRITTVRDAKGAPLRHTI
                     VKTMMRVDLPQPLAPGQSVSFSVDWNYNINDQRKIGGRTGYEFFPKDGNYLYEIAHWF
                     PRMAAYNDVSGWQHKQFLGAGEFTLEFGNYRVSITVPDDHIVASTGVLQNPNAVLTAA
                     QRQRFEQARTAKNPVLIVTPAEALANESHKPTGKKTWVYHADNVRDFAFASSRKFIWD
                     AQGHNVEGNRVMAMSYYPKEGNPLWERYSTHSIIHTLNVYSRYSFTYPYPIAISVNGP
                     VGGMEYPMICFNGPRPQEDGTYSAGTKYGLISVVIHEVGHNYFPMIVNSDERQWTWMD
                     EGLNSFLQYLAEQEWEENYPSWRGEPQNIREYMASENQVPIMTNSESVLQFGNNAYGK
                     PATALNVLRETVLGRELFDFAFKQYSQRWKFKRPMPADFFRSMEDASGVDLDWFWHGW
                     FYTTDHVDLSIERVRLFQPFTGNPEIDKAARMKERSEEPQTLSQERNKNLPKRVDQYP
                     SLRDFYNTYDELVVTERDRAQYQQLLQALTPRERELLQTGLNFYVVDLKNIGGLVSPV
                     IFDIEYMDGTHEMMRIPAEIWRYNNTEVSKLITTNKEIRSISLDPRLETADVDMANNS
                     FPRRPVKSRFQLFKEGQAPPNPMQLLERKQPSPTPTPTPTQ"
     gene            complement(3315..4559)
                     /locus_tag="VGB76_08460"
     CDS             complement(3315..4559)
                     /locus_tag="VGB76_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0098973.1"
                     /translation="MLEHSMKFFVKTFARTIAIVLSAMLLLASLAFDRSAQAQTPAQT
                     PTPPPQQQQQPPATPTPAPPSMEPVRGDDVQDDAVELVETNLVNVLFNATDRNRRFVT
                     TLRQEDLQIFENDVPQSVSLFQRMTDLPLSLAILIDVSGSQERTLPDEKEAAHAFIDS
                     VMRPDRDRAAIISFMGYATIEQDLTGDRASLHRAIEGLFVAPLPPRELESIATATPPV
                     VPAADAATGDDAYDPREARSTAIWDAIWATSRDLLANTPTGTRRAIILLSDGKEDSNS
                     PLKKQDAIDAAIQADASIYSIGIGDYEFDEGALKRISERTGGRAFFPEDEAQLNAAFA
                     QIQQELRTQYLLAYSPTNKARDNTFRRLRLELVNPELRKEKLKLTYRNGYFARPPRAA
                     GATPERAPKARLAKPPKRSKKR"
     gene            complement(4806..5114)
                     /locus_tag="VGB76_08465"
     CDS             complement(4806..5114)
                     /locus_tag="VGB76_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098974.1"
                     /translation="MSAQGLGTAASTAADTITDTLDNLGTGGDATRTDVYEKVLKTID
                     NTTVESTGFSMFHILTIGSIGASLALFFTGNKQAGIFVGLWPPTFLALKSVVDKSNKQ
                     "
     gene            complement(5114..5308)
                     /locus_tag="VGB76_08470"
     CDS             complement(5114..5308)
                     /locus_tag="VGB76_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098975.1"
                     /translation="MTKLDTEQSSERQPATQVQAATKKRARPLAGFFIAGASLALLFG
                     LAALKGRVKDDNTDRERGLD"
     gene            5496..6782
                     /gene="eno"
                     /locus_tag="VGB76_08475"
                     /pseudo
     CDS             5496..6782
                     /gene="eno"
                     /locus_tag="VGB76_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            6965..7147
                     /locus_tag="VGB76_08480"
     CDS             6965..7147
                     /locus_tag="VGB76_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098976.1"
                     /translation="MLQRKHVWLLSVVTISAAYLSYRELTSRGGFVSGLMIGVLMGSL
                     LMVWLEMFKAKKAAKA"
     gene            7203..8756
                     /gene="gpmI"
                     /locus_tag="VGB76_08485"
     CDS             7203..8756
                     /gene="gpmI"
                     /locus_tag="VGB76_08485"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY0098977.1"
                     /translation="MTDRQGPLALIVLDGFGYSPQREGNAIAQASLPFYTEILEKYPH
                     TLLQASGECVGLPTGVMGNSNVGHLCLGAGRVLRTDVERINHEIASGEFLTNAALNQA
                     LDNALHNNRALHLMGLLSDGLVHSSQEHLYALLRLAHDRGLQHVYVHCFLDGRDTPPM
                     SAYGYVEALQVKCAEIGCGRIASVCGRYYAMDRDKRWERTERAYRMLIHAEGERVIDP
                     VEAIKKSYEHSVSDEFIEPMVVTRAREEGEPVATIQDGDSVIFFNFRADRARQITSAL
                     AVSGFDSFDVSGRPPVEYVCFSEYDKTFNLPVAFPKTQPYNILADVFASVGVRNYRMA
                     ETEKYAHVTYFFNGGTEREYPHERRLLIPSPKVATYDLAPEMSAFKLTDKILRAIEEG
                     ETDAFIVNFANPDMVGHTGNLQKTIKAVEYVDTCLGWIMKAMRTARGRTLITADHGNC
                     EQMVDPTTGQPHTAHTTNLVPFHLIDEASVGLPLREGGALEDVAPTMLGLLGLDKPAE
                     MTGRDLREL"
     gene            8812..9471
                     /locus_tag="VGB76_08490"
     CDS             8812..9471
                     /locus_tag="VGB76_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098978.1"
                     /translation="MNADLLIPLLTFGGTLLLVVTGLLNFSVFVRQLRAAREQLETAR
                     RQMESARQQPEIQLVQRAMSETSDHLRILIERPYLRPYFYENKHWAQGDTANSDEVKA
                     MAELLLNNFASAIIHSAVFPQYPVRGVEQTIRFHLRNSPALRDFLLEGFDRFPLAGLA
                     LLCLKNETKAEAERDLHKLIAAAGQDAREKARRERLLHHLQSTDEAGAIELAKYSLEL
                     L"
     gene            9578..10513
                     /locus_tag="VGB76_08495"
     CDS             9578..10513
                     /locus_tag="VGB76_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098979.1"
                     /translation="MSMTKAAQQYLKRLEYRLHRARVGAGKGRFSNFGEEQILQKYIA
                     QLLPAGASRTAVDIGAGDGVKGSNTYALFSNGWRGVGFEGSNRKVCKLAGAYKYYPQV
                     SAANCVVTPENVVPLLEAYNIEREFGVLSLDIDSYDYWVLDALLAEFRPRIVVTEINE
                     KIPPPIRFVVNYDPEFQMTHHFFGYSIASLSELAARHNYAILEVEYNNAFLAPLELPG
                     IVARDAALAYREGYAHRPDRREKFRANANMEILHTLSPSDAVKFLDEFYAQHRGKYEL
                     SSGEAAGDPLERTSQQPETQTNAQGDELVRETARS"
     gene            10553..11074
                     /locus_tag="VGB76_08500"
     CDS             10553..11074
                     /locus_tag="VGB76_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098980.1"
                     /translation="MWLIQWAKSRSEKNEFGVPIPNFAQVTQTLYRGALPDADGYRGL
                     VEKLNVARVCSIIEHERSEDMQRALGSGITEWKHIPFSDRDAPAADKVREWLDFIRTS
                     ETGGAIFTHCRGGRHRTGMLIGVYRVIDCAWTKEQAYAEMMRFGWYSALGHQPLLDWF
                     FHEFDPKEFEKQG"
     gene            complement(11155..12195)
                     /locus_tag="VGB76_08505"
     CDS             complement(11155..12195)
                     /locus_tag="VGB76_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098981.1"
                     /translation="MDTLIKIIYIGFGGTAVVIACVYLIWRAKSVYKLKLAGPPPQRA
                     HRKSIWRDPGAVEELDFYAGPGGESGAPAPPFQFVAEHSTGSNPCVSVRDARGRVWRV
                     KWGDEVRSETFATRMAWAAGYYVEVAYFVPEGMIDGAAGLTRARECVGEDCKFTDARF
                     ELDEEGVTKLFDEHGWAWDDNPFVGTRELSGLKIMLMLTSNWDNKDVRDVARGSNTAV
                     FEYALEGGLVEARYLIIDWGASMGKWGSNLFRSKWDAAGYEAQTPEFVAGVEDGIVQW
                     NYLGQRTAEARDNISVEDVRWLSQYICRLTDAQLRDGLRASGATAEEIATFTRAIRAR
                     LDTLKRIGEEQG"
     gene            12297..12713
                     /locus_tag="VGB76_08510"
     CDS             12297..12713
                     /locus_tag="VGB76_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0098982.1"
                     /translation="MNRVWPDELPVVQVRVARPTRSLQELIRFYCEGLGLAIVGSFTA
                     HAGYSGVMIGLPGSDYHLEFTEREEPEADYAPPSRDNLLVFYIPERAALERVAARLAA
                     MGYASVAPENPYWHERGVTIEDPDGWRVVLMNTSGI"
     gene            12844..13878
                     /locus_tag="VGB76_08515"
     CDS             12844..13878
                     /locus_tag="VGB76_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0098983.1"
                     /translation="MMMNFDPQNILVIDFGQLGDVVLSLPALHAIRAKFPRARITVAV
                     GKPGKPVVEMSNAADETLVVDRVSLRDGSKPLSIWRIGRLVKDVRARRFDFVIDLHSL
                     SETNLLGFLSGASQRLYARRPTRSLDFLSNFRPRPAIEDRSKHAVDRYLDVVAPLGVE
                     AVTRVPRLRTRVEDVRAVDEMLRRERAGTDAPLIGMFPGAGHPSRCWPLARFSELAQR
                     LENNDGVRTLVFAGPEERPLVKAMRAQFPRSAVIFDRLSVPQLAAAAARVSVFVSNDT
                     GPMHIAAAVGTPVVILMSEHPMLDAYVPPGERHRVLHRPGIAQITTDEAYTATRSAFT
                     SERATSLFAG"
     gene            14262..15593
                     /locus_tag="VGB76_08520"
     CDS             14262..15593
                     /locus_tag="VGB76_08520"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0098984.1"
                     /translation="MLKKIPLSLSALFLLALNVSATNAGTRTVPAPLLVSSHAARAIG
                     GDEETDRSRDGLQGPVRRVRTETSKLMSKGGKLTEGARAVLETATYDMKGAKIDTAYF
                     LGASGSLTGKEVYKYDDRGNIVEMTLSNADGSVLSREKYEYEFDAMGNWTKMTTFVAI
                     IENGQMSFEPSEVTYRIIAYYMEDATAKKLQTAALSSNTNAPANVSPASMVSNAPAST
                     SNNAPTSTLNNKPAAPAVTAPVKPGAAAPSSSPQPKMTGAPTAMTNTPAANPSAANMS
                     AANAKAAANNAAPPVKRANNAPVLPTVSAAPTSSSNLTLGMPNVAAASNNSSAPVVKA
                     TDDVPPPPVKPVVKAPVRPVSGGVLNGKALEMPKPMYPEVARRAKMTGVVSVEVVVDV
                     SGKVIGARAVSGPELLRDAAERAARQAKFTPALLSGQPVRVAGVINYNFSL"
     gene            15633..16727
                     /locus_tag="VGB76_08525"
     CDS             15633..16727
                     /locus_tag="VGB76_08525"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0098985.1"
                     /translation="MAFTGQLNDLALSELIEFFCNQRKTGRLKVDYALAPGVFFIKDG
                     DLVDAKVGALNGTEAVFFALTLPSASFDFSAGMQSSRRTINDSWASVVLEGLRRIDEG
                     IMPDASQAFASEEEIDEATIEYLNCIQSAKEAAPAAATPPPRDAAKDTDAADDSFPLP
                     MSQTLEATQDSSAGKRKFVVAGVAAALVLACVVAAVPLSKRFARPQASVNSVAPAATQ
                     STNDTATAQGDNSATPTASTDPDAPATDAAATDAAASEAAAFTARREREARLERQRKL
                     EAKRDAAAHEASNEATAPEVAAPKPAPVGAKTVRVSVSYDEAGRVTQTSVVGSSPGAE
                     AYASTAVRVARGRRFPSGKAGSAVVTIPIN"
     gene            complement(16854..18080)
                     /locus_tag="VGB76_08530"
     CDS             complement(16854..18080)
                     /locus_tag="VGB76_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0098986.1"
                     /translation="MFLTATATAPEHIVRELLRAEKVRRAFQFFKEHAAKFTEEQAAI
                     CEIPAPPFGEEARAEYLAVRFRQCGLAETTIDAEGNCIALRRGANLHPLICISAHLDT
                     VFPAGTNFTVRHEGTRMFAPGIADDGCGLVALLALTSALEETRIETGGSILFVGTVGE
                     EGEGNLRGARYLLTKGEWAREIDAFVSLDGPGLEGITHRAVGSRRYRVRYTGAGGHSW
                     GDFGVPNPVHALGRTVARLIAYPAPKSPRTTFNVGRVEGGAGVNCIAQEAWMDVDLRS
                     ESPDELQRLDAYFRRAAREAAGEENDARRPGAPPLELEMKLIGDRPGGETPQSAPLVR
                     LAEEATRALGSSVRFDCSSTDSNIAISLGLPAITVGAGGLSANSHTLDEWYDPRGREL
                     ALNRALLLLLGTVGMK"
     gene            complement(18198..19412)
                     /locus_tag="VGB76_08535"
     CDS             complement(18198..19412)
                     /locus_tag="VGB76_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HEY0098987.1"
                     /translation="MPASELIYDWNEIEPSHPAPGRHIGFDDETLRDGLQSPSVCEPS
                     VEKKLELLHLMNDLRIDTADIGLPGAGGTHAAGVERLAREIVEQRLQIRPNCAARTHQ
                     NDIRPIAEISQRAGIAIEACTFIGSSPVRFYAEDWTLDKLLQLTEDAIKFAVSEGLPV
                     MYVTEDTTRADPETIRALYTAAIRAGARAVCVCDTVGHSTPDGARAVVRFVKRIIEEQ
                     GGGIRLDWHGHQDRGLGVINSIAAIEGGADQVHGSALGMGERVGNTPMDQLLVNLKLM
                     GWIDNDLSKLSDYCHAAESSCGWNIPVNYPVFGRDAFRTATGVHAAAVIKSFRKGDTE
                     LADLIYSGVPAGLFGMRQVIEVGPMSGKSNVVYWLESRGIEASDERVARIYDKAKSAS
                     GVLGEEEMMQLV"
     gene            19906..20535
                     /locus_tag="VGB76_08540"
     CDS             19906..20535
                     /locus_tag="VGB76_08540"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEY0098988.1"
                     /translation="MDEFIAQGNDEHLSILHRTTPGWIEVIAGSMFSGKSEELIRRLR
                     RAKIARQRVQVFKPHIDTRYSHDHIVSHSEMRHESGVVTSAAEILAQVEANTDVVGID
                     EGQFFDNELVSVANELARRGLRVIIAGLDQDYTGRPFEPMPQLLAIAEYITKTHAICV
                     RCGQPANYSQRIVELEGQVVVGAGDSYEARCRRCFVPHADAPTSYIQEQ"
     gene            20797..21867
                     /locus_tag="VGB76_08545"
     CDS             20797..21867
                     /locus_tag="VGB76_08545"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HEY0098989.1"
                     /translation="MKSQTLVKVLGLFLLAVFAPLAFVAVNTAHSAAAVGLPQAKKDR
                     VHAGDYRISGPYTHNNLAVFLIHGKDKLAGKTFLTLQEALKQRKVVVYETKNVNELAI
                     ENLSRTEEVYVQSGDIVKGGQQDRMLGVDLVVPPRSGKISIGAFCVEQGRWQKRGKEE
                     STHFTSSNDRVATKALKLAANKTKSQGEVWSKVEEAQGKLSANVGRRVNAPISNSSLQ
                     LTLEDRAVQETADGYVKALSHVADKHADVIGYAFAINGKINSADVYAANALFRKLWPS
                     LLKASAVEAIAELQKNQKFEAVSAAEVGAFLDDAEQGAATERDVTRRVQMITRESKVN
                     ILFETRDRNAKGPWLHRSYIKK"
     gene            22151..23095
                     /locus_tag="VGB76_08550"
     CDS             22151..23095
                     /locus_tag="VGB76_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098990.1"
                     /translation="MHALKISKTLTGILLFTLTLAGAACQPPAANTNVANVNANAAGN
                     TNAAATPVTSAATIETREPEQYQATLVFSATAEGKPQALKLPIEVARSGDNRRYAFNN
                     IPVLGQVIFLDRADKRYLILPGRKQYAEITPELIGFDVRSLTPGQMVTVLQQQKGVER
                     VGEEQREGRTVVKYRYAATAKTASQAGDVAAESFIYVDKETGLPLHADLTGQSSGNVQ
                     GVSRAHLVADMQNIQTTVDPALFELPTGLTKLSEEQIKQQVATIGQFLQFALSALNSQ
                     MGNAPASGGTTATPPSPPPTAGGGASPSTSPGASPPAH"
     gene            23114..23908
                     /locus_tag="VGB76_08555"
     CDS             23114..23908
                     /locus_tag="VGB76_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098991.1"
                     /translation="MKYILLLLVGALLGAALTFYIFVGAPRMKQAQTGAPLQAPEAAG
                     DPPGTAVLTLDEKFFDTLLGTIFRDLSAPAFRLAAMPKEMPVAGAQPGLFFVQAQEGG
                     GGCTNQVVLAQEGSGARTTVSLKNGEIIAPLAFSGSYNVFGNCMNFRGAAESNITLYF
                     KPEEQTLYGQITISSVNLDNISPLASPLVTSFVQTAINQKVNPLVLMRGAQLSLNVPV
                     QASNGTLKAQAKDVRAEIKDGALRLHVTYDFSGARAAGDAPAPPQS"
     gene            24006..>25439
                     /locus_tag="VGB76_08560"
     CDS             24006..>25439
                     /locus_tag="VGB76_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098992.1"
                     /translation="MRRSRLEAQQRRWGLAAALALAVIALVPQLQMLKARGVSAWHGS
                     HLSFFYDEAAYAAYINALIDGRPRRNDPYTGRDDAPEARQPESLFSIQFVPAYAVALP
                     ARLLGFDASTAFIILSCLASFASALALYGLLAAITDDDALAATGALSVLCFASLTQKI
                     VRTLRGLQTAYLPLPFLRRYLPAAPLPLFFLFCFLIWRALKAEDRRAANVASILAGLS
                     FALLVFSYFYLWTAAAAWLFCLALLWLAARPPNWRYDLRVFAIVGAIALAALAPYFYL
                     LSHRAPSMDTAQALVFSRRPELFRTTELICALALLILALGAWRGRVKLRERAALFAAS
                     FALMPFVVFNQQVITGRMLQPVHYEQFIANYVCLLALALASLLVWRGLKTSGGAGEAG
                     WRIPRVALAACALAACGWGAGEAFFATRQQLEVNLAVDEARPVGLRLREIARLNSPPE
                     SAAAGARPIVFFDNVGQIDRLPGVAPQA"
BASE COUNT         4947 a         7832 c         7710 g         4950 t
ORIGIN      
        1 gcgagttcat aactcgcacg cgccgcttca atttttatgc gaccggcaat aactactgcg
       61 gcgcgagcgt ctggaaggtg tcgccgtttt tgaaggtgag ggcgcgtttg cggttctcct
      121 gcttgacgtt gacgaggttg acctgatcgt cgtagaggtc gaagagaaat tggtttcgga
      181 gttgcgcgcc cgcgaagccg tcgggcatgc gcgcctcgaa gtagagccac gcgctgtcaa
      241 cccctgcgtc cagcccgacc caggagagtt tcagcgtctc gcccttcgcg tttttgatct
      301 ggaaagtctc ttgcaggtaa gcgatgagca gcggttcggc ctcctgcttg cggtcgaggc
      361 ggacgctctt gccgctgcgc ttgccgagga tcatctccag atcgtccgga aaggtgcgca
      421 gcgtgatttg cagcgacgct tcctgcgcgt tgtagtcggc ctccgcaaaa ctcgtgtgga
      481 acttgtgcgc cgccgccgtc gccgggacga tgcgcggcga agtcaaagcg caggcgacga
      541 gcgcgagact cagcatgtag acgttgaact tcataaatca actttctcta aaacaaacga
      601 tggagaaaag cagaattcag aatccagaag tcagaataga ccgtgcgcgt ctcttgcttc
      661 tgactcctgg attctgtttt ctgaattccg gttcatcatt catcatttat tgcgtcggcg
      721 ttggcgtcgg cgtcggcgag ggttgtttgc gttcgagcaa ttgcatcggg ttcggcggcg
      781 cctgtccttc tttgaagagt tggaagcgcg acttgacggg gcggcgcggg aagctgttgt
      841 tcgccatgtc cacgtcggcg gtttcaaggc gcgggtcgag cgagatggaa cgaatctctt
      901 tgttcgtcgt gatcagcttc gagacttccg tattgttgta acgccatatc tcggcaggga
      961 tgcgcatcat ctcgtgcgtg ccgtccatgt attcgatgtc gaagatgacg gggctgacga
     1021 ggccgccgat gttcttcaag tcaacgacgt aaaaattgag tccggtctgg agcagttcgc
     1081 gctcgcgcgg cgtgagagct tgcaaaagtt gctgatactg cgcgcggtcg cgttcggtga
     1141 cgacgagttc gtcgtaggtg ttgtaaaaat ccctgaggct cggatactgg tcgacgcgtt
     1201 tcggcagatt cttgttgcgc tcctgcgaaa gcgtctgcgg ctcttcgctg cgctctttca
     1261 tgcgcgcggc cttgtcgatt tcggggttgc ccgtgaaggg ctggaagagg cgcacgcgtt
     1321 cgatggagag gtcaacgtgg tcggtggtgt agaaccagcc gtgccagaac cagtcgaggt
     1381 caacgcccga agcgtcttcc atcgagcgga agaaatcggc gggcatcggt cgcttgaatt
     1441 tccaacgctg cgagtattgc ttgaaggcga agtcgaacag ttcacgaccg agcacggttt
     1501 cacgcaggac gtttaaggcc gtcgcgggtt tgccgtaagc gttgttgccg aactgcaaga
     1561 cggactccga gttcgtcatg atcggcacct gattctcgct cgccatgtac tcgcgtatgt
     1621 tctgcggctc gccgcgccat gaaggatagt tctcttccca ttcctgttcg gccagatatt
     1681 gcaggaagga gttcaagccc tcgtccatcc acgtccactg gcgttcgtct gagttgacga
     1741 tcatcgggaa atagttgtgg ccgacttcgt gtatgacgac ggagatgagg ccgtacttcg
     1801 tgcccgccga gtaggttccg tcttcctgcg ggcgcggccc gttgaagcag atcatcggat
     1861 actccatgcc gcccacgggg ccattcacgg agatcgcaat cgggtaagga taggtgaacg
     1921 aataacgcga atagacgttc agggtgtgaa tgatggagtg cgtcgagtag cgttcccaca
     1981 aaggatttcc ttccttcggg taatacgaca tcgccatcac gcgattgcct tcgacgttgt
     2041 gcccctgcgc gtcccagatg aacttgcgcg acgaagcgaa ggcgaaatcg cgcacgttgt
     2101 cggcgtgata aacccacgtc ttcttgcccg tcggtttgtg cgattcgttg gcgagagcct
     2161 cggcgggcgt cacgatgagg acgggattct tggccgtgcg cgcctgttcg aagcgttgac
     2221 gctgtgccgc cgtcaagacg gcgttcgggt tttgcagtac gcccgtcgag gcgacgatgt
     2281 gatcgtcggg cacggtgatc gagacgcggt agttgccgaa ctccagcgtg aactcgcccg
     2341 cgcccaagaa ctgtttgtgc tgccagccgg aaacgtcgtt gtaagcggcc atgcgtggaa
     2401 accagtgcgc gatctcgtag aggtagttgc cgtccttcgg gaaaaactcg tagccggtgc
     2461 gcccgccgat ctttcgctga tcgttgatgt tgtagttcca atcgacggag aaactcacgg
     2521 actggccggg cgcgagcggc tgcggcagat cgacgcgcat catcgtcttg acgatggtgt
     2581 gtctgagcgg cgcgcccttg gcgtcgcgca ccgtcgtgat gcgatagccg ccctcgaagc
     2641 tgcgcgtgta gagcaggttt tcgatctggt tcaggggaac gcgctcgaag ttgggcgcag
     2701 tgctcgtcag gcgcgcatcg gaatcctgct tccagatgtt ctggtcgagt tgcagccaga
     2761 gataggggag cgcgtccggc gattgattat ggtaagtgat agtctcggag gcgatgatgc
     2821 gttgattggc atcgtcgagt tcgacgttga tgttgtaatc ggcgcgctgc tgccagtagg
     2881 aacgaccggg tgcgcccgaa gccgcgcgct gttcgttcgg cgtcggcaaa atctcttcca
     2941 attgcttgaa cttatcactc ggttttgtct tcgactgcgc aggggcgtgg gcgggagcga
     3001 gcaggcagca cagcactgcg aacacaaagg agcgcatgat tttagtcaac atagatgtga
     3061 atgtcctttt accaaagaga ctaaacttgc cgtcacagca ggcacggtca ggatcatccg
     3121 ccaaccctgc aaggaggggt gctaaacatg ctggcggcaa cttgacggaa agggtaacga
     3181 actctgccgg acagtatata tacgaggcgc gaaaggcaaa agttaaagcc ccggcatgaa
     3241 gcataaaaag ccgatccggt gttccatttt ttcggatgcc gcgcgcgata actttctgca
     3301 cttacgataa aactctatct tttcttcgac cgtttcggag gcttcgccag acgcgctttc
     3361 ggcgctcgtt cgggcgtcgc tccggcggcg cgcggcgggc gcgcgaagta gccgttgcga
     3421 taggtaagtt tcaacttttc cttgcgcagt tcggggttga cgagttcgag ccggaggcgt
     3481 cgaaacgtgt tgtcgcgcgc tttgttggtc ggcgaatagg cgaggagata ttgcgtgcgc
     3541 agttcctgtt gaatttgcgc gaaggcggcg tttaactgcg cttcgtcttc gggaaagaaa
     3601 gcgcgcccgc ccgtgcgctc ggagattcgc ttgagcgcgc cctcgtcgaa ttcataatcg
     3661 ccgatgccga ttgaatagat tgacgcgtcg gcttgaatgg cggcgtcaat ggcatcctgt
     3721 ttcttgagcg ggctgttgga atcttccttg ccgtcggaga gcagaatgat ggcgcgtcgc
     3781 gtgcccgtcg gggtgtttgc cagcaggtcg cgggaggtcg cccagatggc gtcccaaatg
     3841 gcggtcgaac gcgcctctct gggatcatac gcgtcgtcgc ccgtcgcggc gtcggcagcg
     3901 ggaacgacgg gcggcgtagc ggtggcgata gactccaact cgcgcggcgg cagaggcgcg
     3961 acgaatagac cctcaatcgc cctatggaga ctcgcgcggt cgcccgtcaa atcctgctca
     4021 atcgtggcgt agcccataaa cgagatgatg gcggcgcgat ctctatccgg gcgcatcacg
     4081 gaatctatga aagcgtgcgc ggcctctttt tcatccggca gcgtgcgctc ctgcgagccg
     4141 ctcacgtcga tgagaatggc gagcgagagc ggtaggtcgg tcatgcgctg gaagagggag
     4201 acgctctgcg gcacgtcgtt ctcaaagatt tgcaagtctt cctgccggag cgtggtgacg
     4261 aagcgacgat tcctgtccgt ggcgttaaag aggacgttga cgagattggt ttcgacgagt
     4321 tcgacggcgt cgtcctgcac atcgtccccc ctcacaggtt ccatgctcgg cggcgcgggc
     4381 gtcggggtcg cgggcggctg ttgttgttgc tgcggcggcg gagttggcgt ctgcgcgggc
     4441 gtctgcgctt gcgcgctccg gtcgaaagcg agagacgcga gaagcagcat cgcggataga
     4501 acgatagcga tagttctggc gaaggtcttg acgaaaaatt tcatggaatg ctcaagcatc
     4561 tccccgtcga attttctccg cgcgaaaggc gatccattat cgttgtcacg gcgtccgtcc
     4621 tttgatgcgg acagggaacg gcgcgcgtgc tcgtcacggg agaggttttt cttattctaa
     4681 cctctcaggg cgcgcgcgcg gaaacggagg cgaaataaga agggcgcgac cagttgcctg
     4741 accgcgccgc ctcttccccc tgtgctcgat aaccccttgt taacccgctt gctttttgct
     4801 gccggttact gcttgttgct cttgtccacc accgatttca gagccagaaa ggtgggcggc
     4861 cagagtccga cgaaaatccc tgcctgcttg ttcccggtga aaaagagtgc gagcgatgcg
     4921 ccgatggagc cgatagttaa aatatgaaac atgctgaagc ccgtggattc aacggtcgtg
     4981 ttatcgatgg tcttcaagac cttctcatac acgtccgtcc gcgtggcgtc gccgcccgtg
     5041 cccaaattgt cgagcgtatc cgtaatcgta tccgcagccg ttgacgctgc cgttcccaat
     5101 ccttgtgctg acattaatct aatccccttt cgcgatccgt gttgtcgtct ttgacgcgcc
     5161 ctttaagggc ggccaggccg aagagcagcg cgagcgatgc cccggcgata aagaagcctg
     5221 cgagcgggcg ggcgcgcttt ttcgtcgccg cctgcacttg cgtcgcgggc tgtcgctccg
     5281 aactctgttc cgtgtccagc ttcgtcatat cccttctgtc gccgccgagc aattatcctt
     5341 ataaagaact ttcatcgcac agttgtatcc aaactcatgc gattctgtgc ggagcgtgct
     5401 tttactttga ctgtccgtga cgaatatgag atagatttga cttattcaaa aacttcagca
     5461 ggcgtaaatt tcaggtcaag cgacgggagc aaactatgag tttgattgag tccatacatg
     5521 cgcgcgaaat tctggactcg cgcggcaatc cgacaatcga ggcggaagtg tctttaatcg
     5581 gcggcgaaca ggggcgcgcc gcagttcctt cgggcgcatc cacgggcgaa cacgaagccg
     5641 tggaactgcg cgacggcgac cgtgcgcgct acggcggcaa gggcacgcgc aaggccgtcg
     5701 aaaatatcaa cgagacgatt gcccacgaac tcgaaggctt cgacgcgctc gaccagaccg
     5761 aagttgacgc cgcgctcatc gcgctcgacg gcacgccgac gaaatccaaa ctcggcgcaa
     5821 acgcgctgct cgccgtctcg ctcgccacgg cacgcgcggc ggcgacctac cttgagatgc
     5881 cgctctaccg ctatctcggc ggcacgaacg cgcgcacgct gcccgtcccc atgatgaaca
     5941 tcatcaacgg cggcgcgcac gccgacaaca acgtagactt tcaggagttc atggtgatgc
     6001 ccgtgggcgt cgccagtttc ggcgaggctc tgcgcgtggg cgcggagatt ttccacgcgc
     6061 tcaagtccgt cttgaagaag agcggcctcg tcacgagcgt cggtgacgaa ggcggcttcg
     6121 cgcccaattt gcgttcgaac gaagaggcga ttgagacgat cctcgaagcc gtcgcggctg
     6181 ccggatacaa gacgggcggc gatgtcctgc tcgcgctcga ccccgcggcg agcgagtttt
     6241 acgacggcaa aaattacgtc ttcaaaaaat ccgaccagcg caaactcacg agcgacgaga
     6301 tggtcgccta ctggcaatcg tggaccgagc aatacccgat catctccatc gaagacggca
     6361 tggctgagaa cgactgggac ggctggaagg ctttgacgga cgccgtagga gggcgcgtgc
     6421 aactcgtcgg cgacgacctg tttgtgacga acacggaatt tttgcgtcgc ggcatcgagc
     6481 gcggcgtcgc caattccatt ctgatcaaag tcaaccagat cggcacgctc accgaaacgc
     6541 tcgactgcat cgaactggcg cgcaccaatc gtcgcaccgt catcatctcg catcgttcgg
     6601 gcgagacgga agacaccttc atcgcagacc tcgccgtcgc caccaacgcc gggcagatca
     6661 agacgggcag tttgtcgcgc accgaccgca tcgccaaata caatcaactc ctgcgcatcg
     6721 aagaagacct ccgcgcctcc gctcgcttcc ccggcatggc cgctttttat caactcgaac
     6781 aggtggctgc ggcgaagccc gcgaccaaga agagtcaagg gagtcagggg agtcaaggaa
     6841 gtgagagtaa gggcgcgaac ggcggggcga agaaagcgcg tgcggcgaag cgttgagcgg
     6901 gtgcaaatgg cgaaagcccc caacgtgaga cggagctaca ggccggggag aaaggtatct
     6961 cgtaatgctg caaagaaaac acgtctggct gctctcggtc gtcaccatct ccgccgctta
     7021 tctctcttac cgtgagctaa cttcgcgcgg agggttcgtg agcggtttga tgatcggcgt
     7081 gctgatgggc agtttgctca tggtgtggct ggagatgttc aaggcgaaga aggcggcgaa
     7141 ggcttagacg cgcgctattt tggttgacac gattcggagt tttgctttcg ggagaattga
     7201 cagtgactga cagacaagga ccgctcgcgc tcatcgtgct cgacggcttc ggctacagcc
     7261 cgcagcgcga gggcaacgct atcgcgcaag cctcgctgcc cttctacacg gagattctgg
     7321 agaagtatcc gcatacgctc ttgcaggcgt ccggcgaatg cgtgggcttg cctacagggg
     7381 tcatgggcaa ctccaacgtc ggccacctct gcctcggcgc gggtcgcgtg ctgaggacgg
     7441 acgtggagcg tatcaaccac gagatcgcgt cgggcgagtt tttgacgaac gccgcgctca
     7501 atcaggcttt ggacaatgcg ctgcacaata atcgcgcgct gcatttgatg ggtctgctct
     7561 cagacggcct cgtccattcg tcgcaggagc acctctacgc gctcctgcgc ttggcgcacg
     7621 acagaggctt gcagcacgtc tatgtccact gctttctcga cgggcgcgac acgccgccga
     7681 tgtccgccta cggctacgtc gaagcgttgc aggtgaagtg cgcggagatc ggctgcgggc
     7741 gcatcgcctc ggtctgcggg cgttactacg cgatggaccg ggataagcgt tgggagcgca
     7801 cggagcgcgc ctaccgcatg ttgattcatg ccgaaggcga gcgcgtgatc gatccggttg
     7861 aagcgatcaa aaagtcgtac gagcatagcg tctcggacga gttcatcgaa ccgatggtgg
     7921 tcacgcgtgc gcgcgaagag ggcgagccgg tggcgacgat tcaggacggc gactccgtta
     7981 tcttcttcaa cttccgcgcc gaccgcgcac gccagatcac gagcgcgctc gccgtctccg
     8041 gcttcgactc gttcgacgtg tccggtcgtc ccccggtcga gtacgtctgc ttctccgagt
     8101 acgacaagac gtttaatctt cccgtggcct tcccgaagac gcagccctac aacattctgg
     8161 cggacgtgtt cgcttcggtc ggcgtgcgca actatcgcat ggccgagacg gagaagtacg
     8221 cgcacgtcac atactttttc aacggtggaa cggagcgcga gtacccgcac gagcggcgtc
     8281 tgctcatccc ctcgcccaaa gtggcgacct acgatctcgc gcccgagatg tcggccttca
     8341 aactgacgga caaaatcctg cgcgccatcg aagagggcga aaccgacgcc ttcatcgtca
     8401 acttcgccaa cccggatatg gtcggccaca cgggcaacct ccagaagacc atcaaagccg
     8461 tggagtacgt agacacatgc ctgggctgga tcatgaaagc catgcgcacg gcgcgcgggc
     8521 gcacgctcat caccgccgac cacggcaact gcgaacagat ggttgacccc acgaccgggc
     8581 agccgcacac cgcgcacacc accaacctcg tccccttcca tctcatagac gaagcctccg
     8641 tcggcctgcc cctgcgcgaa ggcggcgcgc tcgaagacgt agcccccacc atgctcggac
     8701 tgctcggatt ggacaagccc gctgagatga ccgggcgcga cctgcgcgaa ctatagagtc
     8761 gaggtagagt caaggtgtag cccgctttaa gattccggca ggagtaattt ggtgaatgct
     8821 gacctgttaa ttcctctgct cacgttcggc ggcacactcc tgctggtcgt aaccggcctt
     8881 ctcaatttct ccgtcttcgt gcgacaactg cgcgccgcca gggagcagct cgaaactgca
     8941 cggcggcaga tggagagcgc gcgccagcaa cccgagattc aactggtgca gcgggcgatg
     9001 tcggaaacct ccgatcatct cagaatcctc attgagcggc catacctgcg cccttacttt
     9061 tatgagaaca agcattgggc gcaaggcgac acggcgaaca gcgacgaggt gaaggcgatg
     9121 gccgaactgc ttctaaacaa cttcgcgtcc gcgatcattc attcggcggt cttcccgcaa
     9181 tatcccgtcc gtggggtcga gcagacgatc cggttccacc ttcgcaacag ccccgccctg
     9241 cgcgatttcc tgttggaagg attcgaccgc ttcccgctcg cagggctggc tctgctctgc
     9301 ctgaagaacg aaacaaaagc cgaagccgaa cgcgatctac acaaactcat cgccgccgcc
     9361 gggcaggacg cgagggagaa ggcgcgccgt gagaggctcc tgcaccacct gcaatctacg
     9421 gatgaagcgg gcgcaatcga gttggcgaag tacagtctgg aattgctctg aactgatgcc
     9481 cgcccgcctt cgccagacgc ctaagaagag ccgtcgcgcg ctggccggaa tacgtccggc
     9541 ggcgcgattc ctacgacatc gtttataatc gcgcgcgatg agcatgacga aggcggcaca
     9601 acaatatctg aagcgtctgg aatacaggct gcaccgcgca cgcgtggggg cgggaaaggg
     9661 acgcttttcc aatttcggcg aggagcagat tttacagaag tatatcgcgc aactgctgcc
     9721 tgcgggcgcg agccggacgg cggtagacat cggcgcaggc gacggcgtga aaggttcgaa
     9781 cacttacgcg ctctttagca acggctggcg gggcgtgggt tttgagggca gcaatcgtaa
     9841 ggtgtgcaag ctggcagggg cttataaata ttacccgcag gtgtcggctg cgaattgtgt
     9901 agtgacgcct gagaacgtcg tgccgttgct cgaagcctac aatatcgagc gggagttcgg
     9961 cgtgctctcg ctcgacatag atagttacga ttactgggtg ctggatgcgc tgctcgcgga
    10021 gtttcgcccg cgcatcgtcg tcacggaaat taacgagaag ataccgccgc cgatcaggtt
    10081 cgtcgtgaac tacgacccgg agtttcagat gacgcaccac ttcttcggct acagcattgc
    10141 gagcctctcg gagttggccg cgcgccacaa ttacgccatc ctcgaagtgg aatacaacaa
    10201 cgcgtttctc gcgccgcttg agttgcccgg catcgtagcg cgcgatgccg cgctcgccta
    10261 tcgtgaaggc tatgcgcacc gccccgaccg ccgggaaaaa tttcgcgcca acgccaacat
    10321 ggagatactg cacacgctca gtccaagcga cgccgtcaag tttctcgacg agttttatgc
    10381 acagcatcga ggcaagtatg agttaagcag cggcgaggcg gcgggcgacc cgctcgaacg
    10441 cacgtcgcag cagcctgaga cgcagactaa tgctcagggc gacgagttgg taagagagac
    10501 tgcgcgcagc tagcggcgtt gcggcacagg gagcgagaag gagaatgaga aggtgtggtt
    10561 gattcagtgg gcgaaatcgc gttcggagaa gaacgagttc ggcgtgccga ttcctaactt
    10621 cgcgcaggtc acgcagacgc tctacagggg cgcgctgccc gacgcggacg gctatcgcgg
    10681 cctcgtcgag aaactgaacg tggcgcgcgt gtgcagcatc atcgagcacg agcgaagcga
    10741 ggatatgcaa cgcgcgctcg gttcgggcat caccgaatgg aagcacatcc ccttctccga
    10801 ccgcgacgcg cccgccgcag acaaagtcag ggagtggctc gacttcatcc gcacctcgga
    10861 gacaggcggc gcgatcttca cgcactgtcg cggcggcagg catcgcacgg gcatgctcat
    10921 cggagtctat cgcgtgattg attgcgcgtg gacgaaggaa caggcatacg cggagatgat
    10981 gcgcttcggc tggtacagcg cgctcggaca ccaaccgctc ctcgactggt tcttccacga
    11041 attcgacccg aaagagtttg agaagcaggg atgaacgcgg aaggatgaac gcggaaggta
    11101 agacaagcag acaagcggct cttgtcttct tattcatcct tcatcgttcc gcgttcatcc
    11161 ttgttcttcg ccgattcgtt tgagggtgtc gaggcgtgcg cggatggcgc gtgtgaaagt
    11221 ggcgatctct tcggcggttg cgccgctggc gcgcaggccg tcgcggagtt gggcatcggt
    11281 gaggcggcag atgtattgcg agagccagcg cacatcttcg acgctgatgt tgtcgcgcgc
    11341 ttcggcggtg cgctgtccga ggtagttcca ctgcacgatg ccgtcctcga ctccggcgac
    11401 aaactccggc gtctgcgctt cgtacccggc ggcgtcccac ttgctcctga acaggttgct
    11461 gccccacttg cccatcgacg cgccccaatc aatgatcagg tagcgcgctt caaccagacc
    11521 gccttccaga gcgtactcga agacggcagt gtttgagccg cgcgccacgt cgcgcacgtc
    11581 tttgttgtcc cagttcgacg tgagcatcaa catgatcttc aagccgctca gttcgcgcgt
    11641 gccgacgaaa gggttgtcgt cccaagccca accgtgctcg tcgaaaagtt tcgtcacgcc
    11701 ttcctcgtcg agttcgaagc gggcgtccgt gaatttgcaa tcttcgccga cgcattcgcg
    11761 tgcgcgcgtg agtccagccg cgccgtcgat cataccttcg gggacgaagt aggccacttc
    11821 cacgtagtaa ccggctgccc aagccatgcg cgttgcgaaa gtttcggagc gcacttcgtc
    11881 gccccacttg acgcgccaga cgcgcccgcg cgcatcgcgc acggacacgc aggggttcga
    11941 cccggtagag tgttcggcca cgaattggaa gggcggcgcg ggcgcacccg actcgcctcc
    12001 gggtcctgcg taaaagtcga gttcttcgac cgcgccgggg tcgcgccaga tgctcttgcg
    12061 gtgcgcgcgt tgcggcggcg gccctgcgag tttgagttta tagacggatt tagcgcgcca
    12121 gatgagatag acgcacgcga tgacgacggc ggttccgccg aagccgatat agatgatctt
    12181 gattaacgta tccatcgttt ggctgtcagt tgattcctta aaatctacta tagcatctgc
    12241 tactctgaag cccgaaaaag ggcttcctta aatgctttca cgccggagcg agattgatga
    12301 acagagtttg gccggatgaa ttgccggtgg tgcaggtgcg cgtggcgcgc ccgacgcgca
    12361 gtttgcagga gttgatcagg ttctactgcg aaggactcgg cctcgcaatc gtcggttctt
    12421 tcacggcgca cgcgggatat tcgggagtga tgatcggact gcccggcagc gactatcacc
    12481 tggagtttac ggagcgcgaa gagccggagg cggactacgc gccgccgagc cgcgacaacc
    12541 tgctcgtgtt ttatattccc gaacgcgctg cgcttgaacg cgtcgcggcg cgactggcgg
    12601 cgatgggcta cgcgtccgtc gcgccggaga acccttactg gcacgagcgc ggcgtgacca
    12661 tcgaagaccc ggacggctgg cgcgtcgtcc tgatgaacac ttcgggcatc taagacggcg
    12721 cagcatcctc tccagaggag aactttcacg gtcgctccgt acgttatacg agtagacgcg
    12781 ccgtgcggcc tgacgcagcg cagacgcgcg caagctgtta ttattttcgt taacagttat
    12841 ttcatgatga tgaatttcga cccgcagaac atcctcgtca tagacttcgg ccaactcggc
    12901 gacgtggtct tgagtctgcc cgcgctccac gcgatccgcg cgaagtttcc gcgcgcacgg
    12961 atcaccgtgg ccgtggggaa gccgggaaag cccgtcgtcg agatgtcgaa cgcggcggac
    13021 gagacgctcg tggtagaccg tgtgagtctg cgcgacgggt cgaagccgct ctcgatctgg
    13081 cgcatcggtc gtctcgtcaa agatgtgcgt gcgcgccgct ttgatttcgt catagacttg
    13141 cacagtctgt cggagacaaa cttgctcggc tttctgtcgg gcgcgtcgca aaggctctac
    13201 gcgcgacgcc ccacgcgctc gctcgatttc ctctcaaact ttcgcccgcg ccccgccata
    13261 gaagaccgct cgaagcatgc cgtggatcgt tatctcgatg tcgtcgcgcc gctcggcgta
    13321 gaggcggtga cgcgtgtgcc gcgcctgcgg acgcgcgtgg aagatgtgcg cgcggtggat
    13381 gagatgttgc gcagggagcg cgccggaacg gacgcgccgc tcatagggat gtttccgggc
    13441 gcggggcatc cgagccgttg ctggccgctc gcgcgcttct ccgaactcgc gcagagactt
    13501 gagaataacg acggcgtgcg cacgctcgtc ttcgcagggc cggaagaacg cccgctcgtc
    13561 aaagcgatgc gcgcgcagtt tccgcgctcc gcagtcatct tcgaccgcct gagcgtcccg
    13621 caactcgcgg cggcggcggc gcgcgtctcc gtctttgtct cgaacgatac ggggccgatg
    13681 cacatcgctg cggcggtcgg cacgcccgtc gtgatcctga tgagcgagca cccgatgctc
    13741 gacgcatacg tgccccccgg cgagcgtcat cgcgtgctgc accgccccgg catcgcacag
    13801 atcacgaccg acgaagctta cacggccacg cgctcggcct tcacctccga acgcgccacc
    13861 tccctcttcg ccggttaaac acgcgctttc gcacgcgccc ctcttcttcc gctaaatgct
    13921 gcatctacta cgattctttg tccaatcgaa gtttgctctg gacaaaaatt gtttaaccgt
    13981 gtaatactat tttcgctgct gccaaagcag ccgcggcggg cgacttcaac aaaatccacg
    14041 ccccacgccg agccgtccag cacgaacacc ggataaaatt tggcttaaac agttcgcacg
    14101 cgccccgtgc ggctgtcact ggttcacgag cgagttaggc gtcccccgcg cctccttttg
    14161 ctttcgcgag cggggcgaaa agaagaattt cagtcaacct cgccgcttct gccccatcag
    14221 ttgcccctca ccccgacaca aaacttttgg agtcaattgt catgctcaaa aaaatccccc
    14281 tttcgctgtc cgccctcttc ctcctcgcgc tcaacgtctc cgcgaccaac gcaggcacgc
    14341 gcacagtgcc cgcgcccctg ctggtttcgt cccacgctgc cagagcaatc gggggcgacg
    14401 aggagacaga ccgcagccgc gacggcttgc aaggccccgt ccggcgcgtg cgtacagaga
    14461 cttcgaaact gatgagcaag ggcgggaaac tgaccgaagg cgcacgcgcc gtgttggaga
    14521 cggcgaccta cgacatgaag ggcgcgaaga ttgacacggc ctattttctc ggcgcgagcg
    14581 gctcgctcac gggcaaggaa gtttataaat atgacgaccg cggcaacatc gtcgagatga
    14641 ctttgtccaa cgcggacggc tcggtcttga gcagagagaa gtacgagtat gagttcgacg
    14701 cgatgggcaa ctggacgaag atgacgacct tcgtggctat catcgaaaac ggccagatga
    14761 gtttcgagcc gtcggaagtg acctaccgca tcatcgccta ctacatggaa gacgccaccg
    14821 cgaagaaact tcagacagca gccctctcaa gcaacacgaa cgcccccgcc aacgtctcgc
    14881 ccgcgagcat ggtgagcaac gcccccgcaa gcacgtccaa caacgcgccc acgagcacgc
    14941 ttaacaataa gccagccgcg cccgccgtca ccgcgcccgt caagcccgga gccgccgcgc
    15001 cgtcctcatc gccgcagccc aaaatgacag gcgcgcccac cgcaatgacg aacacgccag
    15061 cggctaaccc gtcagcggca aacatgtcag cggcaaacgc gaaggcagcg gctaacaacg
    15121 ccgcgccgcc cgtcaagcgc gcgaacaacg cgcccgtgct gccgacagtc tccgccgcgc
    15181 ccacttcatc ctcaaacctg acgctcggaa tgccgaacgt cgcggcagct tcaaacaaca
    15241 gcagcgcgcc cgtcgtcaaa gcgacggacg acgttccccc gccgcccgtg aagcccgtcg
    15301 tcaaagctcc cgtgcgcccc gtctccggcg gcgtcctgaa cggcaaagcg ttggagatgc
    15361 cgaagcccat gtatccggag gtagcgcgcc gcgcgaaaat gacgggtgtg gtaagcgtgg
    15421 aagtggtggt tgacgtatcc gggaaagtta tcggcgcgcg cgccgtgagc ggtcccgagt
    15481 tgctgcgcga cgccgccgaa cgcgccgcac gtcaagcgaa atttacgccc gcgctgctct
    15541 ccggccagcc cgtacgcgtc gccggggtta tcaactacaa cttctcgctc taatcgaact
    15601 tctcagcccc gactaaagaa ggaacaaaac cgatggcctt taccggacaa ctcaacgacc
    15661 tcgccctgag cgagttgatc gaatttttct gcaaccagcg caagaccggg cgtttgaaag
    15721 ttgactacgc gctcgcgccc ggcgtcttct tcatcaaaga cggcgacctc gtggacgcca
    15781 aggtcggcgc attgaacggc acggaagccg tcttcttcgc gttgacttta ccgagcgcgt
    15841 cgtttgattt cagcgcgggg atgcagtcgt ctcgccgcac gatcaacgat tcgtgggcga
    15901 gcgtcgtgct cgaaggtttg cgccgcatag acgaaggtat catgcccgac gcctcgcagg
    15961 cgtttgcctc cgaagaggag atcgacgaag cgaccatcga atatttgaat tgcatccaga
    16021 gcgcgaaaga ggccgccccg gcagcggcga cgccgccgcc gcgcgacgcc gcgaaggata
    16081 ccgatgcggc ggacgactcg tttccgctgc ccatgtcgca gacgctcgaa gcgacgcagg
    16141 atagcagcgc gggcaagaga aagtttgtcg tcgccggggt cgccgccgcg ctcgtgctcg
    16201 cctgcgtcgt tgccgccgtc cctttgagca aacgtttcgc gcgccctcag gcgagtgtca
    16261 actccgtcgc gcccgccgca acgcaaagca cgaacgacac cgcaaccgcg cagggcgaca
    16321 acagcgcgac gcccaccgcc tcgaccgatc cagacgctcc cgcgacggat gctgccgcga
    16381 cggacgctgc cgcctcggaa gccgccgctt tcaccgcgcg gcgcgaacgc gaagcgcgcc
    16441 ttgagcgtca gcgcaaactt gaggcgaagc gagacgccgc cgcgcatgaa gcctcgaatg
    16501 aagcgaccgc gcccgaagtg gccgctccca aacccgcgcc tgtcggggcg aagaccgtgc
    16561 gcgtcagcgt ctcttacgac gaagccgggc gcgtcacgca aacctccgtc gtcggctcaa
    16621 gccccggcgc ggaagcctac gcttcgaccg ccgtgcgcgt cgcgcgcgga cgccgcttcc
    16681 cctccggcaa agccggttcc gcagtcgtta cgatcccgat caactaaggg agaggggaac
    16741 aggtaagagg taaaggtcta atacaaaaga taaaaagcgg aggcagcttt cgagtgctgc
    16801 gctccgcttt tcttttcctt tacccttttc ctcttagcct ttccccgtct gcttcatttc
    16861 atcccgaccg ttcccagtaa gagcaacagg gcgcggttga gcgcgagttc gcgcccgcgc
    16921 gggtcgtacc attcgtcgag cgtgtgagag ttggcggaga ggccgcccgc gccgacggtg
    16981 atggcgggca ggccgagcga gatggcgatg ttggaatcgg ttgacgaaca atcgaagcgc
    17041 acgcttgaac cgagcgcgcg cgtcgcttct tcggcgagac gcacgagcgg cgcgctctgt
    17101 ggagtctcgc cgccgggacg gtcgccgatg agtttcattt cgagttcgag gggcggcgca
    17161 ccgggacggc gcgcgtcgtt ctcctcgccc gcagcttcgc gtgcggcgcg gcgaaagtat
    17221 gcgtcgaggc gttgcagttc gtccggcgat tccgagcgca ggtcaacgtc catccaggcc
    17281 tcctgcgcga tgcagttgac gcccgcgccg ccttcgacgc gcccgacgtt gaacgtggta
    17341 cgcggacttt tcggcgcagg ataagcgatg agccttgcca ccgttcgacc gagcgcgtgc
    17401 acaggattcg gcacgccgaa gtcgccccac gaatggccgc ccgcgcccgt gtacctgaca
    17461 cgataacggc gcgacccgac ggcgcgatgc gtgatgccct ccaaccccgg cccgtcgaga
    17521 ctcacgaagg cgtcgatctc gcgcgcccac tcgcctttgg ttaaaagata acgcgcgccg
    17581 cgcaggttgc cttcgccttc ctcgcccacc gtcccgacga acaggatcga ccctcccgtc
    17641 tcgatgcgcg tctcttcgag cgcggacgtg agcgccagca gtgcgacgag gccgcagccg
    17701 tcgtcggcaa taccgggcgc aaacatgcgc gtgccttcgt gtcgcacggt gaaattcgtc
    17761 cccgccggaa agaccgtatc gagatgcgcg ctgatacaga tgagcgggtg cagattcgcc
    17821 ccgcgccgca acgcgatgca gttgccctcg gcgtcaatcg tcgtctccgc gagtccgcac
    17881 tggcgaaaac gcaccgcgag atattccgca cgcgcctctt cgccgaacgg cggcgcgggt
    17941 atctcgcaga tcgccgcctg ctcctcggta aacttcgccg cgtgctcctt gaagaattga
    18001 aacgcacgcc gcaccttctc cgcgcgtaga agttcgcgga cgatatgttc gggagcggtt
    18061 gcggtagctg tgagaaacat aagaaagcag tgacgagtga caagtgacgt gtgacgagat
    18121 caaaggcaag tttaaagaca agtttctggt tttaacttgt cacttgtcac tcgtcactcg
    18181 tcactgttct cttgtcgcta gactaattgc atcatctctt cttcgccgag cacgcccgac
    18241 gcgctcttgg ccttgtcata gatgcgcgcg acgcgttcgt cgctggcttc gatgccgcgc
    18301 gattcgagcc agtagacgac gtttgatttg ccgctcatcg ggccgacttc gatcacctgc
    18361 cgcatgccga agagtccggc gggcacgccc gaatagatga ggtctgcgag ttcggtgtcg
    18421 cccttgcgaa aactcttgat gaccgccgcc gcgtgtacgc ccgtcgccgt gcggaaggca
    18481 tcgcgcccga agaccggata gttgaccgga atattccagc cgcaactcga ctctgccgcg
    18541 tggcaatagt cgctgagctt cgacaggtcg ttgtcgatcc agcccatgag tttgagattg
    18601 acgagcagtt gatccatcgg cgtgttgccc acgcgttcgc ccatgccgag cgcagaaccg
    18661 tgtacttgat ccgcgccgcc ctcaatcgcc gcgatggaat tgataactcc caaaccgcga
    18721 tcctgatggc cgtgccagtc gaggcgtatg ccgccgccct gctcctcgat gatgcgcttg
    18781 acgaagcgca cgacggcgcg tgcgccgtcc ggcgtcgaat ggccgaccgt gtcgcagacg
    18841 cagacggcgc gtgcgcccgc gcgaatggcc gcagtgtaga gcgcgcgaat cgtctccggg
    18901 tcggcgcgcg tcgtgtcttc ggtcacgtac atgacgggca agccttccga gacggcgaac
    18961 ttgatggcgt cttcggtgag ttggagcaac ttgtcgagcg tccaatcttc cgcgtagaag
    19021 cggacgggcg acgagccgat gaaagtgcag gcttcgatgg cgatccctgc gcgctgcgaa
    19081 atttcggcga tggggcgaat gtcgttctgg tgcgtgcgcg cggcgcagtt ggggcgaatc
    19141 tgtagtctct gttcgacgat ctcgcgcgcc agacgctcga cgcccgccgc gtgtgtgccg
    19201 cccgcgcccg gcaggccgat gtccgccgtg tcgatccgca ggtcgttcat caggtgcagg
    19261 agttccaact ttttctcgac cgaaggctcg cacacggagg gcgattgcag cccgtcgcgc
    19321 agcgtctcgt cgtcaaagcc gatgtgacga ccgggcgcag ggtgcgacgg ttcgatttcg
    19381 ttccaatcgt agatcaattc acttgctggc acttctcgct tacctccgtt gtacgggaaa
    19441 ttgtcgcgcg ttcgctggca tgttctggat ggaggaatta caacaagggg cgtgtcgaat
    19501 gtcaagcaag agcacccgga cataaagccc tgcccggcgc agccacagag gaagcgatga
    19561 gtgatgtggg cggaatgata aaccggagaa gcaggcgagc aagttactgc tttccttcat
    19621 cgctcatcct tgcacgttca tcatttctct tctctgtgcc tgcgtcggtc agggcttcaa
    19681 cctcctcctt cgacttccgg cggcgtgcgc ttttgactcc gcaaccaact ctgttatagc
    19741 cttcgtcgcg cttcccggtt ggtgaacgaa gaggccgctt cccgctcgtc atcacatccc
    19801 gctcaggcga agttgttttg tgctgccttg caccaatcaa ggcgacggag ggcgagcgcg
    19861 cgacggttcg actatttatt aaatcagaca ggaattgttg acggcatgga tgagtttatc
    19921 gcgcagggta acgacgaaca tttgagtatt ctgcatcgca cgacgcccgg ctggatcgag
    19981 gtgatcgccg gttcgatgtt ctccggcaag tccgaagagt tgatccgccg cctgcgccgc
    20041 gccaagatcg cgcgccagcg cgtgcaggtc ttcaagccgc acatcgacac gcgctactcg
    20101 cacgaccaca tcgtctcgca ttccgagatg cgccacgaat ccggcgtggt gacgagcgcc
    20161 gccgaaatcc tcgcgcaggt cgaggccaac acggatgtcg tcggcattga tgaaggccag
    20221 ttcttcgaca acgaactcgt ctcggtcgcc aacgaactcg cgcgccgtgg cctgcgcgtc
    20281 atcatcgccg gactcgatca agactacacc ggacgcccct tcgagccgat gccgcaactc
    20341 ttagccatcg ccgaatacat caccaagacg cacgccatct gcgtccgctg cggccaaccc
    20401 gccaactact cccaacgcat cgtcgaactc gaaggtcagg tcgtcgtcgg cgcgggcgat
    20461 tcctacgaag cgcgctgccg ccgctgcttc gtcccacacg ccgacgcccc cacctcatac
    20521 atccaggaac agtgacaagt gacaagtgac gagtgacgag ataagaaagt gaatagtgaa
    20581 ttgtgaatag aatgaagact gactttctct attcacgatt tacgattcac aattcacggc
    20641 tttcactcat cactcattac tcatcactca tcacttttct ttctcgtcac tcgtcactgc
    20701 ttttaaggcg caagaagctc ccgccagcct cgttcaacgg gatgaacccc agaagtgttc
    20761 aagctgttac ccaagttgct aacgaggagt atcgagatga aatcgcaaac gctcgtcaag
    20821 gttttgggac tcttcctgct ggcggtcttc gcgccgctcg ctttcgtcgc cgtcaacacg
    20881 gcgcattccg ccgctgccgt agggttgccg caagcgaaga aggatagagt tcacgccggc
    20941 gactaccgga tttccggccc ttacacgcac aacaacctcg ccgtttttct cattcacgga
    21001 aaagacaagc tcgcgggcaa aacttttctg acgctgcaag aggcgctcaa gcagcgcaag
    21061 gtcgtcgtct acgagacgaa gaacgtcaac gaactggcga ttgaaaacct gtcgcgcacc
    21121 gaagaggtct atgttcaatc cggcgacatc gtgaagggcg ggcagcaaga ccgcatgctc
    21181 ggcgtagacc tcgtcgtgcc gccgcgttcg ggcaagattt cgattggagc gttttgcgtc
    21241 gagcagggac gctggcagaa gcgcggcaag gaagagtcca cgcatttcac ctcttcaaac
    21301 gatcgcgtgg cgaccaaagc cttgaagctt gcggcgaaca agacaaaatc gcagggcgaa
    21361 gtgtggagca aggtggagga ggcgcagggc aagttgagcg cgaacgtggg gcggcgcgtg
    21421 aacgctccca tctccaattc gagtctgcaa ttgacgctcg aagaccgtgc ggtgcaagag
    21481 actgccgacg gttacgtcaa agccctctcg cacgtcgcgg acaaacacgc cgacgtgatc
    21541 ggttacgctt tcgccatcaa cggcaagatc aacagcgcgg atgtctacgc cgcgaacgcg
    21601 ctcttccgta aactctggcc gagtctgctc aaggcgagcg cggtggaggc gattgcggag
    21661 ttgcaaaaga atcagaagtt cgaggccgtc tccgccgccg aagtgggcgc gttcctcgac
    21721 gatgccgaac agggcgcggc caccgagcgg gacgtgacgc ggcgcgtcca gatgatcacg
    21781 cgcgagtcga aggtcaacat cctcttcgag acgcgcgacc gcaacgcgaa ggggccttgg
    21841 ctgcaccgga gctacattaa aaaatgaaac gatgaacgat gaacgatgaa ggatgaataa
    21901 gaaaacagaa tccggaagtc agcagtgaag acctgcgcct gttctattct gacttccgga
    21961 ttctgttttc tctttcctgc tttcatcgtt ccgcgttcat ccttcctcct tcatcgtttc
    22021 cgctgcggtg aatcttaatt cttgatcgcg ccaattcttt ttgtgctatt cttcttcacg
    22081 ctaaattccc ctgcaacaaa atctaatgct cgtgcgattt ttacccgaca accgacagga
    22141 gaagacttct atgcacgcac tgaaaatctc taagactctg accgggattc tgctcttcac
    22201 actcacgctc gcgggcgcgg cctgccagcc gcccgccgcc aataccaacg ttgcgaacgt
    22261 caacgcgaac gcagcgggga atacaaacgc cgctgccact cccgtaacgt ccgccgcgac
    22321 catcgagacg cgcgagcctg aacagtatca ggccacgctc gtcttcagcg cgaccgccga
    22381 aggcaaaccg caagcgctca agctcccgat tgaagtggcg cgcagcggcg acaatcgccg
    22441 ttacgccttc aacaacattc ccgtcctcgg gcaagtcatc tttctcgacc gcgcggacaa
    22501 acgctacctc atcctgccgg ggcgcaagca gtatgcggag atcacgccgg agttgatcgg
    22561 cttcgacgtg cgttcgctga cgccggggca gatggttacg gtgctgcaac agcagaaggg
    22621 cgtggagcgc gtcggcgaag agcagcgcga ggggcgcacg gtcgtcaagt atcgctacgc
    22681 ggcgacggcg aagacggctt cgcaggcggg cgacgtggcc gcagagagtt tcatctacgt
    22741 ggataaagag acgggcttgc cgctgcacgc agacctgacc gggcagtcga gcgggaacgt
    22801 gcagggcgtc agccgcgcgc atctcgtcgc cgacatgcag aacattcaga cgacggttga
    22861 ccccgcgctc ttcgagttgc cgacgggtct gacgaagttg tcggaggaac agatcaagca
    22921 acaggtggcg accatcggcc agttcttgca gttcgccttg agcgcgctga attcccaaat
    22981 gggcaacgca ccggcgagcg gcggcactac ggcgacgcct ccgtccccac caccgaccgc
    23041 aggcggcggt gcgtcgccct ccacttcacc cggcgcgtcg ccgcccgctc actgacgagg
    23101 caaaggaaag aagatgaaat atattctcct gctcctcgtc ggcgcgctcc tgggcgcggc
    23161 tttgacgttc tacatcttcg tcggcgcgcc gcgcatgaag caggcgcaga cgggcgcgcc
    23221 gctgcaagct cctgaggcgg cgggcgaccc gccgggcaca gccgtcttaa cgctcgacga
    23281 gaaattcttc gacacgctgc tcggcacgat cttccgcgac ctgagcgcgc ccgcctttcg
    23341 tctcgccgcg atgccgaaag agatgcccgt cgccggggca cagcccggcc tcttcttcgt
    23401 gcaggcgcag gaaggcggcg ggggctgcac gaatcaggtg gtgctcgcgc aggaaggcag
    23461 cggcgcgcgc accacggtca gtttgaagaa cggagagatc atcgcgccgc tcgcctttag
    23521 cggaagctat aacgtcttcg gcaactgcat gaactttcgc ggcgcagccg aatccaacat
    23581 cacgctctac ttcaaacccg aagagcagac gctctacgga cagatcacca tctccagcgt
    23641 caacctcgac aacatctcgc cgctcgccag cccgctcgtg acgagtttcg tgcagacggc
    23701 gatcaaccag aaggtcaacc cgctcgtctt gatgcgcggc gcacaactct ctctgaacgt
    23761 gcccgtgcag gccagcaacg gcacgctcaa agcgcaggcc aaagacgtgc gcgccgagat
    23821 caaagacggc gcgctgcgcc tgcacgtcac ctacgacttc tccggcgcac gcgccgccgg
    23881 agacgctccc gcgccgccgc agagttagac gcgcgcgccg gtgcaagccc ggacatgatc
    23941 taacgggcgc aagtttactt taagacttgc gcccgttttc gtgtttcaac ttcgaggaga
    24001 gttcgttgcg ccggagcaga cttgaagcac agcaacggcg ttggggattg gcggcggctc
    24061 tggcgttggc agttatcgcg ctcgtgccgc agttgcagat gttgaaggcg cgcggcgtga
    24121 gcgcgtggca cggctcgcac ctctctttct tctacgacga ggccgcttac gccgcttaca
    24181 tcaacgcgct gattgacggt cgcccgcgtc gcaacgatcc ttacaccggg cgagacgacg
    24241 cgcccgaagc gcgccagccc gaatcgctct tctccatcca gttcgtcccg gcctacgccg
    24301 tcgcgctgcc cgcgcggctg ctcggcttcg acgcctccac ggccttcatc attctctcct
    24361 gtctcgcgtc cttcgcttcc gcgctcgcgc tctacgggct gctcgccgcc atcacggacg
    24421 acgacgcgct ggcggcgacg ggcgcgctct ccgtgttgtg cttcgcatcg ctcacgcaga
    24481 agatcgtgcg gacgctgcgc ggactccaaa ccgcttatct ccctctgccg ttcctgcgcc
    24541 gttacttacc cgccgcgccg ctccctctct tttttctctt ctgctttctc atctggcgcg
    24601 cgctcaaagc ggaagacagg cgcgcggcca acgtcgcttc aatcctcgcg ggactcagtt
    24661 ttgcgctgct cgtcttttcc tatttttacc tgtggacggc tgccgccgcg tggctcttct
    24721 gcctcgcgct gttgtggctc gcggcgcgcc cgccaaactg gcgttacgac ctgagagtct
    24781 tcgccatcgt cggcgcgata gcgctggccg cgctcgcgcc ctacttctac ctgctctcgc
    24841 atcgcgcccc ctcgatggac acagcgcagg cgctcgtctt ctcgcgtcgt cccgaactct
    24901 ttcgcacgac cgaactgatc tgcgcgctcg ccctcctcat cctcgcactc ggcgcatggc
    24961 gcggtcgggt caaactgcgc gagcgcgccg cgctcttcgc cgcctcgttc gcgctcatgc
    25021 ccttcgtcgt cttcaatcaa caggtcatca cggggcgcat gctccagccg gttcactacg
    25081 aacagttcat cgccaactac gtctgcctgc tcgcgctcgc gctcgcttcg ctcctcgtct
    25141 ggcgcgggct gaaaacatcg ggtggagcgg gagaggcagg atggaggatt ccgcgcgttg
    25201 ctctggcggc gtgcgcgctc gcggcgtgcg gttggggcgc gggagaagca ttcttcgcca
    25261 cacggcaaca gcttgaggtg aatctggcgg ttgacgaggc cagacccgtc ggcctgcgcc
    25321 tgcgagagat cgcgcgactg aactcgccgc ccgaatccgc cgcagcaggc gcgcgtccaa
    25381 tcgtcttttt cgataacgtc ggccagatag atcggctacc gggtgtcgcg ccgcaagcc
//
LOCUS       DASWNS010000244        18692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_138971, whole genome shotgun sequence.
ACCESSION   DASWNS010000244 DASWNS010000000
VERSION     DASWNS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18692
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138971"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..2619)
                     /locus_tag="VGB76_08565"
     CDS             complement(10..2619)
                     /locus_tag="VGB76_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0098993.1"
                     /translation="MKFSSCRRHPTVSLILTASLIFLAVVQTRAATVPTGFTDTVVAS
                     NLTNATAMAIAPDGRIFVCLQDGSLRVVKNGALLPTPFVNVTVNASGERGLLGVALDP
                     NFASNNFVYLYYTATTPAIHNRVSRFTANGDVAVAGSETVLLDLDNLSGATNHNGGAM
                     HFGPDGKLYIAVGENASPANAQNGNNLLGKMLRINADGTIPEDNPFFNAVAGRNRAIW
                     ALGLRNPYTFNFQPGTGRMFINDVGQNAFEEVNDGIAASNYGWPTCEGFSCSATPPGG
                     YRAPLFVYGHTGAAPTGCAITGGAFYNPVTPQFPAEYVGKYFFADFCSSFIRTIDPAS
                     PATSNSFATGAASPVDLQVASDGSLYYLQRGSTGQLRRIQYPAGQVPPTIGQHPQSQT
                     VAAGQPVTFNVSATGATPLRFQWQRNNADIPGANSDSYTIPSASLSDHGAQFRCIVTN
                     AFGSATSNNATLNVTSNNPAPGTRDLLISEYRLDGPGGLRDEFVELYNNTNEAITVTT
                     PDNSTGWAIVTGRDTAAGVVLETYHVIPNNTVIPARAHYLVAGNAYTLANYGGANRAL
                     ADATSQSDLAGDDAQHPFRGVAIFRTSDANNFTTPNRLDAAGGACADARLSEGTLSGL
                     CLNVGTPTTPSANYSLVRRQPTGSPQDTDNNADDFVLVAVQSPLPSANAGANLAAELG
                     APGAENLSSPIQHNATIKAMLIDPQAASSAPPNRVRAGGTIPNGAFGTLTIRRRFVNK
                     TGATVRALRFRLTGITTLGSPVVSSPQADLRALSSSDAEVQTSAGLLTVKGTLLEQPP
                     AQASGGGLNSTLVVPLAGGALIPNASVDVQFLLGVQQEGSFRFLVNVEALTQPVASEQ
                     VKGASRKGEQVKE"
     gene            4133..4924
                     /locus_tag="VGB76_08570"
                     /pseudo
     CDS             4133..4924
                     /locus_tag="VGB76_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
     gene            5402..8635
                     /locus_tag="VGB76_08575"
     CDS             5402..8635
                     /locus_tag="VGB76_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0098994.1"
                     /translation="MVKNFKFLPSILALLFCLSGVAAFGQETTGEIQGTVKDPAGARV
                     PGVTVTIKGVDVGFNRDITTDEEGYFRARQIPPGIYIVSAAATAGFTGQTRERVQVVL
                     GQATTVDFDVTVGGASADVTVTSDDAGLAVDPTDTKVQESITAREIDSLPKGTNITSL
                     LRTTAAARPEPLSGQFQINGGSGSENSFIVDGQSLENFRTGVLNANNDIPFQSVQEVQ
                     VKSSGFEAEFGGATGGVVNIVTKSGTNDFRGEFGMNFSTPEFNAAQRPILTNTATFDF
                     SSPAAAQPVRVVNNGRDRGTNFFPTAQIGGPIIRDRAWFLAEYTPRIFNATRTTAFVP
                     NPVTGVVTSTQTATAKETYEYARIRLDGSISDQLRVSSSFTWNPIVQRGLLLGNTTAF
                     TTPSFFETGTTQFGTLSGAELAAFQGGRQNSNNFRIEGVYTPNSQLVTTLRYSRGFLN
                     EKLNSYFVPNIPRFRVRQCIGQFAGAGCQTLTGRPNAFQNTNNNNTVNFDVSVRNQVN
                     ADASYLVSGFGGRHEFKGGYEYNKILNDVDRGYADTGRVSLFFGRNTSYPVEFVSALP
                     PFNPNPNAAGVGVLLRIGTVGRAENLNQALYLQDKWQPTERLTFNLGARIEKEDLPAF
                     NGQQTNLRFGFMDKLAPRLGVAYALTSDGKTKISAFYGRFYDRLKFELPRGSFGGDFF
                     RVDYFDIPAGGPFTVESFNVARIIGNYPDQPGGSCSATGIVSTGLTRCQFDFRIPSNV
                     PEFAGLGGVDPDLEPFRQSEFTIEFQREVFTQSVFKTRYLHRNVDQAIEDAGFLTAEG
                     SEAYIIGNPGRGLYAQRARQFGFNRVAEPQRRYDAFQVELDTRFVRNFNLNVNYTLSR
                     LYGNYSGLANSDENGRTSPGVNRNFDLPFVGFTAAGQPDNGRLATDRPHVFKASGTYS
                     HGWFGRNTNTTDLSFFTTAQSGTPQTTFVDVFDIDIPKTRRGDLGRSEMFTQTDLNLT
                     HRYRFGNENRFALAFDFNVLNVFNENNVLTLDTRESSAYYALGVSEVSATGNFVEAVN
                     RLTSVGILNELNESVNHDPDFNISRTYGLPNAFQAPRSVRFGFRFQF"
     gene            complement(9045..10070)
                     /locus_tag="VGB76_08580"
     CDS             complement(9045..10070)
                     /locus_tag="VGB76_08580"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0098995.1"
                     /translation="MLILTAETPATTAPAPRSHLALTATGSAAMQQRNTIRGSIYTPE
                     RRPLENLRVELLDEVDGLVKATYTNGAGQFVFFGLSTGTFHVRVLTTGTDFESRTERV
                     TITGSVIGSPVGGSQSEQVDLYLRYKKGRGAQATVTAAPGTVYVQEVPAAARKVYEQA
                     LKELEDNKRDQALLSLQRAVELFPTYFAALDLLGQEHVKRGNYEGAQPFLTRAVAVNR
                     RSPSTWYALGYAQYKLRQFPAATEALNKSASYNPNSINTQLVLGTVQRRQQQWLAAET
                     HLQRAKALSKTTPVAEVHWQLALLYNQTNRAAAAADELELFLKAQPDSRDEVRIRKVI
                     GDLRRKK"
     gene            complement(10521..12551)
                     /locus_tag="VGB76_08585"
     CDS             complement(10521..12551)
                     /locus_tag="VGB76_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0098996.1"
                     /translation="MAKIKIAPYGSWKSPITSDLIVQGSVGLGAVRVDREDIYWLELR
                     PSEGGRSCLVRRDASGATRDMTALPFNIRTRVHEYGGGDFVVHHGTIYFTNFSDQRLY
                     RQTPDDSKPQPLTPESNNELRYADMIVDASRRRLIAVREDHTAGTHEPTNALVAVSLE
                     PYTKRADTADVDATNADALNVDTPGNGGQVLVSGYDFYSSPRLSPDGKELAWLCWNHP
                     NMPWDGTELWRGDVGADGAITGARRIAGGRDESIFQPEWSPDGRLYFVSDKSGWWNLY
                     RMAQAGAGEPFEALCEMEAEFGQPQWIFGMSTYAFTSASEIICAYTVRGMSSLARLDT
                     ETKQLQRIESPYADIQYLQAAPGRAVFRAGAPDMPPAIVALDLSDPAGAQFEVLRRAS
                     EHEIDEGYLSIAQPVEFPTSNGQTAHAFFYPPRNRDFAAPEGTRPPLLVKSHGGPTSA
                     ASTTLSLNIQYWTSRGIAVLDVNYGGSTGYGRAYRLRLNGTWGITDVDDCANGARALA
                     AAGLVDGERCVITGGSAGGYTTLAALTFRDTFKAGASHYGVSDLGALVRDTHKFEARY
                     LDNLIGPYPERQDIYQARSPINFTDQLSCPVIFFQGLEDKVVPPNQAELMVEALRAKG
                     LPVAYVAFPGEQHGFRRAENIKRALDGELYFYSRVFNFELADPVEPVKIENL"
     gene            12981..13832
                     /locus_tag="VGB76_08590"
     CDS             12981..13832
                     /locus_tag="VGB76_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0098997.1"
                     /translation="MRKKRMGLILDKITLPTEMPRISRGRLLDVLHESMACCTSTVIC
                     GRTGTGKTMLATDFARRSGRRVVWYKVDAPEVDRQTFLRYLIAAVRDQHPGFGRKTLA
                     LLTASSDSMPDAALLAESFVYEMTMLEAADEPLLIVIDDLHLLYDAEWLVPFFGRLLP
                     LLPVEAHMILIGRTLPPAPLWRMRSKQTLRVIEESVLAFTHAEAEELFHSYGLQVRAA
                     GQALAETWGRAAALDAVARAHREASNAETSRGQQQRANSLAAARPVGSRLRLVQSYIE
                     EPSLGAS"
     gene            14213..17611
                     /locus_tag="VGB76_08595"
     CDS             14213..17611
                     /locus_tag="VGB76_08595"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY0098998.1"
                     /translation="MSNRPHRQGKLQTSPLPPPITGAGAGAAATHAFFLRTKLLPPRP
                     TPALLQRPRLMERLSANLAHPVTLVAANAGSGKTTLVADFVRTHARPFVWYQLDRTDA
                     DPFVFLGYLAHGIKGVVPEFGAATLSYLQQSAAELAQQPERAVDVFLNEVLDLVDRQL
                     IFVLDDYHHLGTDTAVHRVVDRLLAYLPDVLHIIIISRDVPPLALARLRAHNELTIID
                     RAELLFTDDETQELFRKVFDLELTPEQLAEYRERTHGWITALQLIRQVAQRQLLARGG
                     REGEAQHRPDLSEILRQSELDIFDYFAEEVFADEAEDAQQLLLRISLLERVEVENCAR
                     LYPDGSCAQLLPALVRRNVFMTVAGSDGRGEEYRLHPLFRDFLRRRLRSEIGQSGVAR
                     EHLRYAEFFVERKQWEQAIRHLLAAEEFARAAVIIAHKGQEWITTGAFTSLISFVEAL
                     PEAAIEAHPRTLFHRAEVARLRGEYETSQTLFRRAATLLNARGDAEGEAEALHSLATI
                     ARRRGDCAGAFTYLDRALELVGEESPVRIKCGNTRGLCYFAMAEWAAAEGEFRAALQL
                     AEEQHDTHYMHLIAHNLGLPAMIRGDFSEAMRWLRRMLRDNRDAPPVPQEAVAYLNLA
                     RCHLYRGELPLCEQHLDHALERCQLFNLIAQRAETFETYGNLHRERGDAARAMEFYGH
                     AARTYEEAGVELARSELWEEQALLNLQIGDTGAARALLERLIAARLPVKDEMALQTAT
                     LAHARVLIALGEHSEARAELEPALRYFHQHGLHYYEAHASLALAACALAEAKEKELME
                     RLRRVLDLSARYDYDYWLRREVVHQPQLFASPDAVALLPPDIREELQVAAQAGAQQTT
                     THVTRPPETAQAVDAALAVPPKPAADLTINMLGPVEIFRDPARPLAADAWTTKRARDI
                     LCFIASRRHRRASKDTIIDTFWGEADFEAVEKNFHPTVSHIRKALNSNQPVKQNFLLY
                     RDSDYLLNQEFSYRVDTEEFDRLVMEAEAAKRAGDTARYLCAYEEALALYRGDFMQGS
                     YDEWVEEQRSYYREQHLRMLEVLAVAAQKNEEWTRSLQLAQRILGDDQFREDVHCMIM
                     RAHAALGNRAAVKDQYEHLRRVLRKELGVEPASETQKIFKGLMS"
     gene            17888..>18692
                     /locus_tag="VGB76_08600"
     CDS             17888..>18692
                     /locus_tag="VGB76_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0098999.1"
                     /translation="MKKIRPAAAVSLALLLLINSVNAGINISNSDGITATGADGINYI
                     GVNGITATGADGVLTFGVNGITATGADGITATGADGITATGADGITATGADGITATGA
                     DGISYPNPNSVTAAKPSGITATGADGITATGADGITISTPDGVQHKADSVFINRPNGI
                     TISTANGLTAIGVNGITATGADGITATGADGITATGADGITATGADGITATGADGITI
                     STASGVTARSADGRFFNVSPNGITIQHADGITATGADGINIPAAEKLSIS"
BASE COUNT         3591 a         5927 c         5628 g         3546 t
ORIGIN      
        1 catccttcat cattccttca cctgttcgcc ttttctcgac gcgcctttca cctgttcgct
       61 ggcaaccggc tgcgttaagg cttcaacgtt gacgaggaag cggaaactgc cttcctgctg
      121 cacgccgagg agaaactgca catccacgct cgcgttcgga atcaacgcgc cgcccgcgag
      181 cgggacgacg agcgtggagt tcaggccgcc gcccgacgct tgcgccggag gctgttcgag
      241 cagcgtgcct ttaaccgtca gcagtcccgc gctcgtctgc acttccgcgt cggacgaact
      301 cagggcgcgc aggtcggcct gcgggctgga gacgacgggc gagccgagcg tggtgatccc
      361 cgtcaggcgg aagcgcagcg cgcgaacggt ggctcccgtc ttgttgacga agcgacggcg
      421 aatggtgagc gtgccgaacg cgccgttcgg aatcgtcccg cccgcgcgca cgcggttggg
      481 cggcgcgctc gatgccgcct gcgggtcgat gagcatggcc ttgatggtgg cgttgtgttg
      541 gatggggctg ctcaagtttt ccgctcccgg cgcgccgagt tccgccgcga ggttcgctcc
      601 cgcgttcgcg ctcggcagcg gactctggac ggctacgagc acgaagtcgt ccgcgttgtt
      661 gtctgtgtcc tgcggagatc ccgtcggctg cctgcgcacg agactgtagt tcgcgctcgg
      721 cgtcgtgggg gtgccgacgt tcaggcacag acccgataaa gttccctcgc tgagccgtgc
      781 atcggcgcac gcgccgcccg ccgcgtcgag ccggttgggc gtcgtgaaat tgttggcgtc
      841 ggacgtgcgg aagatcgcga cgccgcgaaa cggatgctgc gcgtcgtcgc ccgccagatc
      901 gctctggctc gtggcatctg cgagcgcgcg gtttgcgccg ccgtaattcg ctaacgtgta
      961 ggcattgccc gcgacgagat aatgtgcgcg ggcggggatg accgtgttgt tcgggatgac
     1021 gtgataagtt tcgagcacga cgcccgccgc cgtgtcgcgc cccgtcacga ttgcccagcc
     1081 cgtcgaattg tcgggagtgg tcacggtgat cgcttcgttc gtgttgttgt agagttcgac
     1141 gaattcatcg cgcaggccgc cgggtccatc gagcctgtac tcgctgatca agagatcgcg
     1201 cgtgcccggt gcgggattgt tggaggtcac gttgagagtc gcgttgttgc tcgtcgcgct
     1261 gccgaaggcg ttcgtgacga tgcagcgaaa ctgcgcgcca tgatccgaga ggctggcgga
     1321 agggatcgtg taactgtcgg agtttgcgcc ggggatgtcc gcgttgttgc gttgccattg
     1381 aaaccggagc ggcgtcgcgc ccgtcgcgga gacgttgaag gtgacgggtt gcccggcggc
     1441 gacggtctgg ctctgcgggt gctggccgat ggtcggcggc acttgtcccg ccggatattg
     1501 aatgcggcgc agttgccctg tgctgccgcg ttgcaggtag tagaggctgc cgtcgcttgc
     1561 gacctgcaag tcaacgggag aagccgcgcc cgtggcgaag ctgttcgacg tagcagggct
     1621 ggccggatca atcgtgcgaa taaagctgga gcagaaatcc gcgaagaaat atttgcccac
     1681 gtactctgcc ggaaattgcg gcgtgaccgg attgtagaac gcgccgcccg tgatggcgca
     1741 gcccgtgggc gcagcgcccg tgtgcccgta gacgaagagc ggcgcgcgat agcctccggg
     1801 cggcgtcgcg ctgcaactga aaccttcgca ggtcggccag ccgtagttcg atgcggcgat
     1861 gccgtcgtta acttcctcaa aggcgttctg cccgacatcg ttgatgaaca tgcgacccgt
     1921 gcccggctgg aagttgaacg tgtaggggtt gcgcaagccg agcgcccaga tggcgcgatt
     1981 cctgcccgcc accgcgttga agaagggatt gtcctcaggg atcgtgccgt ctgcgttgat
     2041 gcgcagcatc ttgccgagga ggttgttgcc gttctgcgcg ttggcggggc tggcgttttc
     2101 gccgacggcg atgtagagct tgccgtctgg tccgaagtgc atcgcgccgc cgttgtgatt
     2161 cgtcgcgccg ctcaggttgt cgagatcgag gagaactgtt tcgctgcccg cgacagccac
     2221 gtcgccgttg gcagtgaagc ggctgacgcg gttgtgaatg gcgggcgtgg tggccgtgta
     2281 atagaggtag acgaagttgt tcgaggcgaa gttcggatcg agggcgacgc cgagcaggcc
     2341 gcgttcgccc gacgcgttca cggtgacgtt gacgaagggc gtcggcagga gcgcgccgtt
     2401 tttgacgacg cgcaaactcc cgtcttgcag gcagacgaag atgcgcccgt ccggcgcaat
     2461 cgccatcgcc gtcgcgttcg tcaggttgct cgccacgacc gtatcggtga agcccgtggg
     2521 aacggtggcc gcgcgcgtct gcacgacggc taagaagatg agcgacgccg taaggatcaa
     2581 ggagacagtc ggatgcctgc ggcacgaaga gaatttcatc acacaccacc ctcggatcaa
     2641 gattcaaacg atgaggactt ttcgccttgg gggagcttgc ggcgcggggc atcaactgtt
     2701 gacgtgccgg attatcggag gcggcgcgcg aaccgtcaag atcaaaaaga cggcggcctg
     2761 aaagggacgg cgcgcgggcg cgggtttacg agccacgacg catctatgat aaatttccgc
     2821 cagccggaaa ctcttcctac acatcagaaa ttttaaggag aataatatga agcgttttgc
     2881 tgtccgagtc ctcgtctttg cgctcacact ctacagcgtc gccgccgtcg ccgtcgtggc
     2941 gcagacggcg aagcgtccgt tgacgattga tgatctgttg aaagtgcgcc gcgtcggcga
     3001 cccgcaactc tcgcccgacg gcaaatggat cgcctaccag atcagcgatc cggacaaggc
     3061 cgcgaaccgc agccgcaccc aaatctatct cgtctcgatt gacggcggcc aacccgtgca
     3121 actgacgacg ggcgagagtt cggcgagttc gccgcgctgg tcgcccgacg gcaagcgcat
     3181 cgcctacgtc acgggcgggc agattcacac gatggatgcg cgcggcggca acgtcaaaaa
     3241 gatcacttcg atctcgacgg gcgcgggcga tcccgtctgg tcgccggacg ggcgcatgat
     3301 cgctttcgtc tccgacatct atccagactg ccccgacgat gcctgcaacc agaagcgcga
     3361 cgcggaagtg gaagcgaata aagtcaaagc gaaggtcatc gaccgcctgc tctatcgcca
     3421 ctggacgagt tggaaggacg gcaagcgcac gcacgtcttc gtcgtcagtt cggacggcgg
     3481 cgcggcgcgt gacctgacgc ccggcgactt cgacgccccg cccttctccc tcggtggtcc
     3541 caccgactac gccttctccc cggactcgcg cgaactcgcc ttcgcgcgca acacggagaa
     3601 agtagaagcc acttcgacca acaacgacat cttcatcgtc cccgttgcgg gcggcgacgc
     3661 gcagcgcatc acgggcgaca atcccggctc ggatcaatcg ccgctctact cgcccgacgg
     3721 tcgctatctt gcctttcgct cgcaagccac cgcaggcttc gagagcgcgc gctggcgtct
     3781 gatgctctac gaccggcaga cgaagaagct gcgccaactg gcctccacgc acgacgccta
     3841 cgtcgaaggc ttcaccttta cccccgacag caggagcatc tatttcgtct ccggccagcg
     3901 cggtcgccag ccgctctaca tggtcactct gtccgacggg cagttgacga aagttttcga
     3961 cggcttcaac gacgacgtgc gcgtcgcgcc ggatggcgaa acgctcgtct tcacgcgctc
     4021 aagctcgatc cagccgacgg agatttatcg ctatgccccg gagggcgggc accgcgtcgt
     4081 cgcgcaggtc accagaacca acgacgagta tctcgctaat ttcaacctgc cgatggcgga
     4141 ggaagtggag tgggcgggcg cgggcggcac gaagattcac ggctggctct tcaagcccac
     4201 gactcccccg gcgggtggtc gctacccgct cgtcgtcctc attcacggcg gaccgcaggg
     4261 cgcctggaac gacaattggg gctaccgttg gaatccgcaa atcttcgccg ccgcaggcta
     4321 catggtcttc atgccgaacc cgcgcggctc gaccggctac gggcagcagt ttacagacga
     4381 aatctccggc gactgggcgg gcaaagtcta tacggacatc atgaacggcg tggcgcatgt
     4441 cgcctcgcgc cctgaagttg accgcgagcg catcggcgcg gcgggcggct cttacggcgg
     4501 ctagatggtc aactggattc tcggccacaa cgaccatccg caggttaagt tcaaagctct
     4561 cgtctcgcac gcgggcgtct tcaatctggt cagcatgtac ggcgtgacgg aggagttgtg
     4621 gtttcccgaa tgggagttca agggcacgcc ttgggacaac cccgaactct acacgcgctg
     4681 gtcgccgcac ctccacgcca agaacttcaa gacgccgacg ctcgtgacgc acggcgagtt
     4741 ggactaccgc gtgcccatcg gcgagggctt ccagttgttc acctatctgc aaaagcagaa
     4801 cgtgccctcg aaactgctct acttcccgga cgaaggccac tggattcaga aaccacagaa
     4861 ttccgcgctc tggtacaaca cggtcatcgg ctggttcgat cagcacttga aacagaaagc
     4921 ctgagcggga gcgcaacgaa acgcaattga accgaacgct ctgcgcccct ttgtgtcctc
     4981 tgcgtctccg tggtgaatct ctttgaccaa gccttcacca cggagacaca gagaacacgg
     5041 agcgggcacg gagacagtat caagacccgc gcaggggcgg gtcgctattg gttggcagag
     5101 attcgtcgtg tgagtccgtt gagggtctat tcaaaaagct ggacgcttat ccaaaatttc
     5161 gttgaccaaa atgtaaattc agcctccgct cgctccgtcc ggagactcgc acgctgcccc
     5221 gctaatcctc tatttccttt aattttcacc agtttcggag aaagcccacg ggcagcgcgg
     5281 cctgtggact tccaattgca tagttccacg ctatcagaac ccccgtaaat gttctggcag
     5341 ttgtcaggcg cttagggatg ttaacttttc gcaactaacc attttgtatt aggagaaaac
     5401 catggtcaag aatttcaagt ttttaccgtc gattctcgcc ctgcttttct gtctgagcgg
     5461 tgtggccgct ttcggacagg aaacgaccgg tgagattcaa ggcacggtta aagacccagc
     5521 aggtgcgcgc gtgcccggcg tgaccgtcac catcaaaggt gtcgacgtcg gttttaaccg
     5581 cgacatcacg actgacgagg aaggctactt ccgcgcccgg cagattccgc ccggcatcta
     5641 catcgtcagt gccgcggcga ccgctggctt cactggtcaa accagagaga gagtgcaggt
     5701 tgtcctcggt caagcgacca ccgttgactt cgatgtcacg gttggcggcg cgtctgccga
     5761 cgtgaccgtc acgtccgacg atgcgggtct ggcagttgac ccgacggaca ccaaagttca
     5821 agaaagcatc accgctcgtg aaatcgattc gctgcccaaa ggcacgaaca tcaccagctt
     5881 gttgagaacg accgctgccg ctcgtcccga gccgctgtcc ggtcagttcc agatcaacgg
     5941 cggttccggt tccgagaaca gcttcatcgt tgacggccag tcgctggaga acttccgcac
     6001 cggcgtgctc aacgccaaca acgacattcc gttccagtcc gtgcaggaag tgcaagtcaa
     6061 gtcgagcggc ttcgaagccg aattcggcgg cgcgaccggc ggcgtggtca acatcgtgac
     6121 caagagcggc acgaacgatt tccgtggcga gttcggcatg aacttctcca cgccggaatt
     6181 caacgccgcg caaagaccga tcttgaccaa cacggccacc ttcgacttct cgtctccggc
     6241 tgccgcgcag cccgtgcgag tcgtcaacaa cggcagagat cggggcacga acttcttccc
     6301 gaccgcgcag atcggcggcc cgattattcg tgacagagcc tggttcctgg ccgagtacac
     6361 gccgcgtatt ttcaacgcga cgcgcaccac ggctttcgtt cccaaccccg tgaccggcgt
     6421 ggtgacttcc acgcagaccg cgacggccaa ggaaacctac gagtacgctc gcatcagact
     6481 cgacgggtcg atttccgacc aactgcgcgt ctcttcgagc ttcacctgga acccgattgt
     6541 tcaacgcggc ctcttgctcg gcaacacgac cgccttcacc acgcccagct tcttcgagac
     6601 gggcaccacc cagttcggaa ccctgtcggg tgccgagttg gcagctttcc agggcggacg
     6661 ccagaactcc aacaacttca gaattgaagg cgtctacacg ccgaacagcc agctcgtcac
     6721 gacgttgcgc tactcgcgcg gcttcttgaa cgagaagctg aattcgtatt tcgtgccgaa
     6781 catcccgcgt ttccgcgtgc gccagtgtat cggccagttc gcgggtgccg gatgccagac
     6841 cctgacgggt cgtccgaacg cgttccagaa caccaacaac aacaacacgg tcaacttcga
     6901 cgtgtcggtt cgcaatcagg ttaatgccga tgccagctac ctcgtgagcg gctttggcgg
     6961 tcgccacgaa ttcaagggcg gctatgaata caacaagatt ctgaacgatg tggatcgcgg
     7021 ttacgccgac acgggtcgcg tcagcctctt cttcggcaga aacacgtcct acccggtcga
     7081 gttcgtttcc gcgcttcctc cgttcaaccc gaacccgaac gccgctggcg tcggcgtcct
     7141 gctcagaatc ggtacggtcg gcagagccga gaacctgaac caggctctct acctgcaaga
     7201 caaatggcag ccgaccgagc gtttgacctt caacctcggt gcgcgcattg agaaagaaga
     7261 cctgcccgcg ttcaacggtc agcagaccaa cctgcggttc ggcttcatgg ataagctcgc
     7321 tccgcgcctc ggcgtggcct atgccttgac gagcgatggc aaaacgaaga tttcagcgtt
     7381 ctacggtcgt ttctatgacc gcctgaaatt cgaattgccg cgcggctcgt tcggcggcga
     7441 cttcttccgc gtcgactact tcgacatccc ggctggtggt cctttcaccg tcgagagctt
     7501 caacgtcgct cgcatcatcg gcaactaccc ggatcagccg ggcggttcct gctcggccac
     7561 cggcatcgtc agcacgggtc ttacccgctg ccagtttgac ttccgcattc cgtccaacgt
     7621 gccggagttc gcaggtctgg gcggcgtcga tccggacttg gagccgttcc gtcagagcga
     7681 gttcaccatt gaattccagc gcgaagtgtt cacgcagtcg gtgttcaaga cccgctacct
     7741 ccatcgcaat gttgaccagg cgattgaaga cgcgggcttc ctgaccgccg aaggcagcga
     7801 ggcttacatc atcggcaacc ccggtcgtgg cctctacgcg cagcgcgcca gacagttcgg
     7861 tttcaaccga gttgccgagc cgcaacgccg ttacgatgcc ttccaggttg agttggacac
     7921 gcgtttcgtc cgcaacttca acctgaacgt gaactacacc ctgagccgtc tctacggcaa
     7981 ctactccggt ctggcgaact ccgacgaaaa cggtcgcacg tcgccgggcg tcaaccgcaa
     8041 cttcgacttg ccgttcgtcg gtttcaccgc cgccggccag ccggataatg gccgcttggc
     8101 taccgaccgt ccgcacgtct tcaaagcgtc gggcacctat agccacggct ggttcggtcg
     8161 caacacgaac acgaccgacc tctcgttctt caccaccgcg cagtcgggaa cgccgcaaac
     8221 gacgttcgtt gatgtcttcg acatcgacat tccgaagacc cgtcgcggcg atttgggacg
     8281 cagcgaaatg ttcacgcaga ccgacttgaa cctgacgcac cgctatcgtt tcggcaacga
     8341 aaacagattc gcgctcgcgt tcgacttcaa cgtcctcaac gtgttcaacg agaacaacgt
     8401 gctcacactc gacacgcgcg agtcgagcgc ctactacgcg ctcggcgtga gcgaagtctc
     8461 cgcgaccggc aactttgtcg aagcggtcaa ccgtctcacc tcggtgggca tcctcaacga
     8521 actcaatgag tccgtgaacc acgatccgga tttcaacatc agccggacgt acggacttcc
     8581 gaatgcgttc caggctccgc gctcggttcg tttcggcttc cgcttccagt tctagtccac
     8641 atagaccgaa ccggaacctt caaagcccgt ccgatttatt cggacgggct ttttgttttt
     8701 ctccctcacc cgctccggct tcaacccgac gctcacgcgt gacctgcgac ggcggaatcc
     8761 tctacataaa atgcgacagg cgcgacggaa attagcccgc gtgcgtgagc cgtgcgaata
     8821 gcagtcatct caaacagatc gaagcccctg agacgggcga aagactcgca atgccgaaag
     8881 tctttcgccc gtctcagggg cttatcaatt gctctcacga aatagcccac ggcttgcgca
     8941 agccgtgggc tataaatatc gcgttgctga acgtgcgccc gtcgcgtggc tccgcgtcgt
     9001 gcgttcgcac ggttctccga tctaactttg agccggagcg agcactactt cttccggcgc
     9061 aggtcgccga tcacttttct gatgcgcact tcgtcgcgcg agtccggctg cgctttgagg
     9121 aacagttcca gttcgtcggc ggcagcggcg gcgcggttgg tctggttata gagcaacgcg
     9181 agttgccagt ggacttcggc caccggagtg gtcttgctca gagccttggc tcgctggaga
     9241 tgagtttccg cggccagcca ttgttgctgc ctgcgttgga cggttccgag cacgagttgc
     9301 gtgttgatgg agttggggtt gtaagaagcg gacttgttga gcgcttcggt cgcggcggga
     9361 aactgccgga gtttatattg cgcgtaaccg agcgcgtacc aggtggaagg gctgcggcga
     9421 ttaacggcga cggcgcgcgt caggaaaggt tgcgcccctt catagttgcc gcgcttcaca
     9481 tgctcttgtc cgagcaggtc gagcgcggcg aagtaggtgg gaaacaactc gaccgcacgc
     9541 tgcaaactca acagggcttg atctctctta ttatcttcaa gctccttcaa agcctgctcg
     9601 tagactttgc gcgccgccgc cggcacttcc tgcacgtaga cggttccggg agccgccgtc
     9661 acggtcgcct gcgccccacg ccccttttta tagcgcagat acaggtcaac ctgttcggac
     9721 tgtgagccgc cgacaggaga gccgatgaca gagccggtaa tcgtcacgcg ctccgtgcgg
     9781 ctttcaaaat ccgtccccgt cgtcagcact ctcacatgaa acgtgccggt cgaaagtccg
     9841 aaaaagacga actgccccgc gccgttcgtg taagtggcct tcaccaaccc gtcaacctca
     9901 tcgagcaact cgacgcgcag gttttcgagc gggcgacgct ccggcgtata gatcgagccg
     9961 cgaatagtgt tgcgctgctg catggccgca ctgccggtcg cagttaaggc gagatggctt
    10021 cggggcgcag gcgccgtggt ggctggagtc tcagccgtca atatcaacat caaaacgacg
    10081 ctctctatca tttgtcgtgc ctcctacgat aaatcattgt caactacaca tcctcccttt
    10141 gcctcatcat ccctgcccgg ctcgaacctg cggctcgccg tccagccaac tacgcgcttt
    10201 aagcatcgcg cttgggtcga agtcacacgt cgccgcagcc gatggagaaa ccactcagcg
    10261 tccggacgct tctcttcata tccaattcct ctgtgaagcg aattcgctcg attaaggttc
    10321 aaaaaaggtt ttcagagacg ggttaacgag gaagaattgt agcacaagag atgcaccggg
    10381 aagcgcgaat tttttcgccg ccgtgggcaa aattctcgtc cgaacggatt gcgctcgcgg
    10441 gcggttctca tggtcgcggg gaggtcgcgt cgttaataag aattgcctgt gagcgcagtc
    10501 cgttcgggca gggaaaactg ttagagattc tcgatcttga ccggctcaac cgggtcggcc
    10561 agttcgaagt tgaagacgcg cgagtagaaa tagagttcgc cgtcgagcgc gcgcttgata
    10621 ttttcggcgc gacggaagcc gtgctgctcg ccggggaagg cgacgtaggc cacgggcagc
    10681 cccttggcgc gcagggcttc taccatcaac tcggcctgat tgggcggcac caccttgtct
    10741 tcgagtccct gaaagaagat gacggggcag gagagttgat ctgtgaagtt gatgggcgag
    10801 cgcgcttgat agatgtcctg acgttccggg taaggaccga tcaggttatc gaggtaacgc
    10861 gcttcgaact tatgcgtatc gcggacgagc gcgccgagat cgctgacgcc gtagtggctc
    10921 gcgcccgcct tgaaggtgtc gcggaaggtg agcgcggcga gcgtcgtgta gccgcccgcg
    10981 ctgccgcccg tgatgacgca acgctcgccg tcaaccagac ctgcggcggc gagcgcacgc
    11041 gctccgttgg cgcaatcgtc aacgtccgta atgccccagg tgccgttcag tctcaagcga
    11101 tacgcgcgcc cgtagccagt gctgccgccg tagttcacgt cgagcacggc gatgccgcga
    11161 ctcgtccaat attgaatgtt caagctgagc gtggtcgaag cggcggaggt ggggccgccg
    11221 tggcttttga cgagaagcgg cgggcgcgtc ccttccggcg cggcgaagtc gcggttgcgc
    11281 ggcgggtaga agaaggcgtg cgcggtctgc ccgttcgagg tcggaaactc cacgggctgc
    11341 gcgatggaga gatagccttc gtcgatctcg tgttcactcg cgcggcgcag gacttcaaac
    11401 tgcgcgcccg ccggatcgct cagatcaagt gcgacgatgg cgggtggcat gtcgggcgcg
    11461 cccgcgcgaa agacggcgcg acccggtgcg gcttgaagat actgtatgtc cgcgtaaggg
    11521 ctttcgatcc gttgaagttg ctttgtctct gtgtcgaggc gtgcgaggct cgacatgccg
    11581 cgcacggtgt aggcgcagat gatctcgctc gcgctggtga aggcgtaagt tgacatgccg
    11641 aaaatccatt gcggctgtcc gaactcggcc tccatctcgc agagggcttc aaacggctca
    11701 cccgctcccg cttgcgccat gcgatacagg ttccaccagc cgctcttgtc ggagacgaaa
    11761 tacaggcgtc cgtcgggcga ccattcgggt tgaaaaatag actcgtcgcg cccgcccgcg
    11821 atgcgccgcg cgcccgtgat cgcgccgtcc gctcccacgt cgccgcgcca caactccgtc
    11881 ccgtcccagg gcatgttcgg atgattccag cagagccacg ccaactcttt gccgtcggga
    11941 ctcaggcgcg gcgaagaata aaaatcataa ccggagacga gcacctgacc gccgttgccc
    12001 ggcgtgtcta cgttcagcgc atccgcgttc gtcgcgtcca cgtccgctgt gtccgcacgc
    12061 ttcgtgtacg gttcgaggct gacggcgacg agcgcgttcg tgggttcgtg cgtgcccgcc
    12121 gtgtggtctt cgcgcacggc gatcaagcga cggcggctgg cgtccacgat catgtcggcg
    12181 taacgcagtt cgttgttcga ttcgggcgtg agcggttgcg gcttggagtc gtccggcgtc
    12241 tggcgataga ggcgttgatc ggaaaagttg gtgaaataga tcgtgccgtg gtggacgacg
    12301 aaatcgccgc cgccgtattc gtggacgcgt gtgcggatgt tgaaaggcag cgccgtcatg
    12361 tcgcgcgtcg cgccggaggc gtcgcggcgg acgaggcaac tgcgcccacc ttccgagggt
    12421 cgcagttcga gccagtaaat gtcttccctg tccactctga ccgcgccgag tccgaccgaa
    12481 ccttgtacga tgaggtcgga ggtaatgggc gacttccagg agccgtatgg agcaattttg
    12541 attttagcca tgccccacat aatgaaccaa aaagcggcga caagccaagc cccttgctcg
    12601 ccccgggagt attaaatttt ccgcctgccc cgtcgcccgc tccctgcttt caactgaacg
    12661 cgaactgctc cgccgtcttt cagaaactgc tcttatcaaa cgactttcgc caattcctca
    12721 agttctaccg tcgcactcgc ctgctacgag gcgacgtgcg tggcgggcgt gggcgttaaa
    12781 catctcaaga attcctcaag tcccctcgcg catagtcttt acggtaggcg cgcattgcac
    12841 gcacggggca tgagccggag actttagagg cgaacagttt tcagagagac ggcagtttcc
    12901 aaagtggcga caacttaaac ggaacctttg cgcgtctcct caaacgcgcg ttcaaagttc
    12961 cctcttcgtg agaaagagag ttgagaaaaa agagaatggg attgatcctc gataagatta
    13021 cgcttcccac agagatgccg cgcatctcgc gcgggcgtct gctcgacgtg ctgcatgaaa
    13081 gcatggcgtg ctgcacctcc accgtgattt gcgggcgcac gggcacgggc aagaccatgc
    13141 tcgccacgga tttcgcacgc cggagcgggc ggcgcgtcgt ctggtacaaa gttgacgcgc
    13201 cggaggttga ccggcagacg tttttgcgat acctgatcgc cgccgtgcgc gaccagcatc
    13261 ccggcttcgg acgcaagacg ctcgcgctgc tcacggcttc gagcgattcg atgcccgatg
    13321 ccgcgctcct ggccgaatcc ttcgtctacg agatgacgat gctcgaagcg gctgacgaac
    13381 cgctcctgat cgtgatcgac gatctgcact tgttgtatga tgccgagtgg ctcgtcccat
    13441 tcttcggacg cctcctgccg ctcttgcccg tcgaagcgca catgatcttg atcggtcgca
    13501 cgcttccgcc cgcgccgctg tggcgcatgc gctcgaagca gacgttgcgc gtgatcgagg
    13561 agtccgtgct ggccttcacg cacgcagaag cggaagaact ctttcacagt tacggactgc
    13621 aagtgcgcgc ggcggggcag gctctggccg aaacgtgggg acgcgccgcc gcactcgacg
    13681 cggtggcgcg cgcgcatcga gaagcgtcga acgcagagac atcgcgcggg caacagcagc
    13741 gcgcaaactc tctggcggcg gcgcgcccgg taggttcacg tttacggctc gtccaaagct
    13801 acatcgaaga gccttcgctc ggagcgagtt gaagtattcc ggcggcgcgt tcgcgaagcc
    13861 gtccacccac gccggaactg cccggcactt gcacttgaag gttcaaaggg agtaagaagt
    13921 cagtaacttc tagtgacttg aatttagagc gagtttcaaa acctacgccg cgccttccgt
    13981 ctttttcttc gcgccgcaag cgagaaaaaa tttcaggccg gaggcgcaaa gaagcagcga
    14041 aagcggatgg ggtcggggtg attgttcgcc gttttgaaat aatctcccgg tagctttaag
    14101 tgattgcctg cggctcgcgc acgaaagttt aaggtcgcaa acgcagcccg aaccaacttt
    14161 tttattttcg cttggggcaa gcctccacta aaaggaggcc gccgggaggc tgatgagcaa
    14221 caggccgcat cgtcagggca agttacagac atcgccgctg ccgccgccga tcacgggagc
    14281 aggcgcggga gcagcggcga cgcatgcctt cttcctgcgc accaagctcc tgccgccgcg
    14341 ccccacgccc gcgctcctgc aacgcccgcg cctgatggag cgtctgtctg ccaatctcgc
    14401 gcatccggtc acgctcgtcg ccgccaacgc cggttcgggc aagaccacgc tcgtcgcaga
    14461 cttcgtgcgc acgcacgcgc gccccttcgt ctggtatcaa ctcgaccgca ccgacgccga
    14521 cccgttcgtc ttcctcggct atctcgcgca cggcatcaag ggcgtcgttc ccgaattcgg
    14581 cgcggccacg ctctcttacc tgcaacagtc agccgccgaa ctcgcacagc agcccgaacg
    14641 cgccgtcgat gtcttcttga acgaagtgct cgacctcgtt gaccggcaac tcatcttcgt
    14701 cctcgacgac tatcatcatc tcggcacgga cacggctgtc catcgcgtcg tagaccgcct
    14761 gctcgcctac ctgccggacg tgctgcacat catcatcatc tcgcgcgacg tgccgccgct
    14821 cgcactcgcg cgcctgcgcg cgcacaacga actcaccatc atcgaccgcg ccgaactcct
    14881 ctttacagac gacgagacgc aggaactttt tcgcaaagtc ttcgacctcg aactgacgcc
    14941 cgaacaactc gccgaatacc gcgaacgcac gcacggctgg atcaccgcgc tgcaactcat
    15001 acgtcaggtc gcgcagcgtc agttgctcgc acgcggcggg cgcgaaggcg aggcgcaaca
    15061 tcgtcccgac ctctcggaga ttttgcgtca atccgaactc gacatcttcg actatttcgc
    15121 cgaagaagtt ttcgccgacg aagcggagga cgcgcaacag ttgctcctgc gcatctcgct
    15181 tctggagcgc gtggaagtgg aaaactgcgc gcgtctctac ccggacggaa gttgcgcgca
    15241 gttgttgcct gcgctcgtgc gtcgcaacgt ctttatgacg gtcgcgggaa gcgacgggcg
    15301 cggcgaagag tatcgcctgc atcccctgtt cagggatttt ctgcgccgcc gcctgcgctc
    15361 ggagatcggg cagagcggcg tggcgcgcga gcatctgcgc tacgcggaat tctttgtcga
    15421 acgcaagcaa tgggagcagg ccatacgcca tctgctcgcg gcggaagagt ttgcgcgcgc
    15481 cgccgtcatc atcgcgcaca aggggcagga atggatcacg acgggcgcgt tcacctcgct
    15541 catctcgttt gtcgaagcac tgcccgaagc ggccatcgaa gcgcatcccc gcacgctctt
    15601 tcaccgcgcc gaggtggcgc gcctgcgcgg cgaatacgag acttcgcaga ccctctttcg
    15661 ccgcgccgcc acgctcctca acgcacgcgg cgacgcagag ggcgaggccg aagccctgca
    15721 ctcgctcgcc acgattgcgc gccgtcgcgg cgactgcgcg ggcgcgttca cgtatctgga
    15781 ccgcgcgctc gaactcgtgg gcgaagagtc gccggtgcgc atcaagtgcg gcaacacgcg
    15841 cggcctgtgt tacttcgcaa tggccgagtg ggctgctgcc gaaggcgagt ttcgcgctgc
    15901 actccaactc gcggaggagc agcacgacac gcactacatg cacctcatcg cgcacaatct
    15961 cggactgccc gccatgattc gcggcgactt ctccgaggcc atgcgctggc tccgccggat
    16021 gctgcgcgac aaccgcgatg cgccgcccgt gccgcaggag gccgtcgctt atctgaacct
    16081 cgcgcgctgc catctctacc ggggcgaact ccccctttgc gaacagcatc tcgaccacgc
    16141 cctcgaacgc tgtcaactct tcaacctgat cgcgcagcgc gccgaaacct tcgagactta
    16201 cggcaacctc caccgcgagc gcggcgacgc cgcacgcgcg atggagtttt acggccacgc
    16261 cgcgcgcacc tacgaagagg ccggagttga actggcgcgc tccgagttat gggaagagca
    16321 ggcgttgctc aatttgcaga tcggcgacac gggcgcagcg cgtgcgttgc tcgaacgtct
    16381 catcgccgcg cgcctgcccg tcaaagatga gatggctttg cagacggcca cgctcgcaca
    16441 tgcgcgcgtg ctcatcgcgc tcggcgaaca cagcgaggca cgcgccgaac tcgaacccgc
    16501 gcttcgatat tttcaccagc acggccttca ttattacgaa gcgcacgctt ctctcgcgct
    16561 cgccgcctgc gcgctggccg aagcgaagga gaaggaactc atggaacgcc tgcgccgcgt
    16621 gctcgatctc tccgcgcgct acgattacga ctactggctg cgccgcgaag tcgtgcatca
    16681 accgcaactc ttcgcctcgc ccgatgccgt cgcgctcctg ccgcccgaca tacgcgaaga
    16741 actacaggtc gccgcacagg ccggagcgca gcagacaacc acgcacgtaa cgcgcccgcc
    16801 tgagacggcg caggcagttg acgccgcgct cgccgtaccg ccgaagcccg ccgccgatct
    16861 gacgatcaac atgctcggtc ccgtggagat cttccgcgac ccggcacgcc cgctcgccgc
    16921 agatgcgtgg acgaccaaac gcgcacgcga cattctgtgc ttcatcgcct ctcgccgcca
    16981 ccgccgcgcc tccaaagaca cgatcataga caccttctgg ggcgaagccg atttcgaggc
    17041 ggttgagaaa aactttcatc cgaccgtctc ccacatacgc aaagctctca acagcaacca
    17101 gcccgtcaag cagaactttc tgctctatcg tgacagcgat tatctgctca atcaggagtt
    17161 ctcttatcgc gtggatacgg aagagttcga ccgtctggtg atggaagccg aagcggccaa
    17221 gcgcgcgggc gacacggcgc gctatctctg cgcgtatgaa gaggcgctcg cgctctatcg
    17281 cggcgacttc atgcagggca gctatgacga gtgggttgaa gaacagcgtt catattatcg
    17341 tgagcaacat ctgcggatgc tcgaagtgct cgccgtcgcg gcgcaaaaga acgaggaatg
    17401 gacgcgctct ctgcaactgg cgcaacggat tctcggcgac gatcaatttc gcgaagacgt
    17461 gcactgcatg atcatgcgcg cacacgccgc gctcggcaac cgtgcggcgg ttaaagacca
    17521 gtacgagcac ttgcggcgcg tgctgcgcaa ggaactcggc gtcgaaccgg cctcggagac
    17581 gcagaagatt ttcaaggggt tgatgagcta gacggggcgc gcgggaaact gcgactccgc
    17641 cggtctttaa taaacttaag cccctatgag tagattgcgc aggctgcacg gcagcttaaa
    17701 aaagaaggaa aatgtgacaa ctggcggcgt cgagtctggt gaatttatgc tattttgttt
    17761 cccccgactt ttaagctcct acctcgccgt gcgcccgact cccagcgcga gcagaggccg
    17821 acaggtcttc aaaatcaata gcataaattt tggcttccgt gtttgctcaa gcatgatgaa
    17881 tatagctttg aaaaaaatcc gcccggcagc cgccgtctcc ctcgcgctcc tacttttaat
    17941 caattcggtc aacgccggaa tcaacatctc caactccgac ggcatcaccg ccacgggcgc
    18001 ggacggcatc aactatatcg gcgtcaacgg catcacggcg acgggcgcgg acggcgttct
    18061 gaccttcggc gtcaacggca tcaccgccac cggagcggac ggcatcaccg ccacaggggc
    18121 agatggaatt accgcgaccg gggcggatgg catcacggcg acgggtgcgg atggcatcac
    18181 cgctacgggc gcagacggca tctcttaccc gaatccgaac agcgtcaccg ccgcgaaacc
    18241 cagcggcatc acggcgaccg gagcggacgg catcaccgcc acaggcgcgg acggcatcac
    18301 catttcgact cccgacggcg tgcagcacaa ggccgattcg gtcttcatca atcgcccgaa
    18361 cggcatcacc atctcgacgg ctaacggcct gacggccatc ggcgtcaacg gcatcacggc
    18421 gacgggcgcg gacggcatca ccgccaccgg agcagacggc attacggcca cgggcgcaga
    18481 cggaattacc gcgaccgggg cggatggcat cacggcgacg ggtgcggacg gcatcacgat
    18541 ctcaaccgcg tccggcgtca ccgcacgcag cgcggatgga cgtttcttca acgtctcgcc
    18601 caacggcatc acgatccagc acgcagacgg catcacggcc accggcgcgg acggcatcaa
    18661 catccccgcc gccgaaaagc tctcgatctc ga
//
LOCUS       DASWNS010000245        16440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_217951, whole genome shotgun sequence.
ACCESSION   DASWNS010000245 DASWNS010000000
VERSION     DASWNS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16440
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217951"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..451
                     /gene="rpsJ"
                     /locus_tag="VGB76_08605"
     CDS             128..451
                     /gene="rpsJ"
                     /locus_tag="VGB76_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY0099000.1"
                     /translation="MLNEKIRIRLKAYDHRVLDQSTSEIVETAKRTGARVAGPIPLPT
                     SKNKWTVLRSPHVDKKSREQFEIRTHKRLMDILDPTPQTVDALMKLDLPAGVDVEIKA
                     FGRDR"
     gene            545..1204
                     /gene="rplC"
                     /locus_tag="VGB76_08610"
     CDS             545..1204
                     /gene="rplC"
                     /locus_tag="VGB76_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY0099001.1"
                     /translation="MINGIIGKKVGMTQLFAPDGTVTPVTVIKAGPCVVVQTKSAAGK
                     DGYDAVQIGLVEERPVKLKNVNKPMRGHFEKTGAGTPPTRVLKEFRLEGDAGVSVGDT
                     VLVGEQFADGDTIEVIGKSKGRGFAGTVKRHNFNRGPESHGSMNVRAPGSIGQSAYPS
                     RVIKGTRSSGHMGDVRITVKGLTVAHVDAENNLLMIRGAVPGANGTVVIVKKSVRQKK
                     K"
     gene            1295..2011
                     /gene="rplD"
                     /locus_tag="VGB76_08615"
     CDS             1295..2011
                     /gene="rplD"
                     /locus_tag="VGB76_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY0099002.1"
                     /translation="MPTVKVRNLKNEEIADLELSDAVFGAEYNEGLVHAAVKGYLAKA
                     RAGTVGTKTRGDVSGSGKKLWKQKGTGRARIASIRSPLWKGGGNVHGPQAKDWSHNLP
                     KKMRRGALRSALSERLREGNVLILDAFTLGQAKTKDFISALGTLGLEGKTLIVDSHDN
                     DNLLLASRNVKRAKITTSRSLNIYDLLYHEKLVLSRTAAEELGDMLDPKRRTGAHDEE
                     TDEGGTTPDAGAANTTEEAA"
     gene            2015..2320
                     /locus_tag="VGB76_08620"
     CDS             2015..2320
                     /locus_tag="VGB76_08620"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY0099003.1"
                     /translation="MRTVWDIIKSPVITEKALVAKEETQDGKQLLTFRVDKNATKPEI
                     KSAIERIFDVKVASVRTMNYMGKKARRGRFEGRKPSWKKAYVTLKEGEKPLDYGEAI"
     gene            2410..3240
                     /gene="rplB"
                     /locus_tag="VGB76_08625"
     CDS             2410..3240
                     /gene="rplB"
                     /locus_tag="VGB76_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY0099004.1"
                     /translation="MGIKKLTPTSPARRYQTYLTRDELTKGAEPEKSLLEKKTRISGR
                     NNDGHITVRRRGGGAKRFYRIIDFKRDKTGVPGRVTQLEYDPNRSAHIALITYLDGEK
                     RYILAPAGIEVGKTIMSGPEADILAGNALPLKNIPLGTQIHNIELRPGKGGQMARSAG
                     AFAQLVAKEGDYAQLRMPSGEVRRVPVVCMATVGQVGNVEHENVSFGKAGRSRWKGIR
                     PHVRGVAMNPVDHPHGGGEGKTSGGRHPVTPWGQPTRGYKTRSNKRTQKMIVRDRRVK
                     "
     gene            3358..3651
                     /gene="rpsS"
                     /locus_tag="VGB76_08630"
     CDS             3358..3651
                     /gene="rpsS"
                     /locus_tag="VGB76_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY0099005.1"
                     /translation="MARSVKKGPFIDDYLLKMVARIRESGARPPAIKTWSRRSTITPD
                     FVGLTFAVHNGKRHLPVYVTENMVGHKLGEFAPTRTFKGHSGTKAEKGGKVAR"
     gene            3749..4312
                     /gene="rplV"
                     /locus_tag="VGB76_08635"
     CDS             3749..4312
                     /gene="rplV"
                     /locus_tag="VGB76_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY0099006.1"
                     /translation="MDARASAKYLRGSPQKARLVVDMIRGKNVNEALAVLQYTNKRAA
                     DGIEKCLRSAVANATEAAEKANIAVDPDDLWVKACFIDMGPTKNRRRMRPAPQGRAYK
                     ERRHFHHVTVLLSSDEQEALKGRRPSKAGKPQTTAASKTRKKTDGTATAATKTATKRA
                     ASKKPETTAVETPAAAESASGDTATAE"
     gene            4334..4954
                     /gene="rpsC"
                     /locus_tag="VGB76_08640"
                     /pseudo
     CDS             4334..4954
                     /gene="rpsC"
                     /locus_tag="VGB76_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            5186..5614
                     /gene="rplP"
                     /locus_tag="VGB76_08645"
     CDS             5186..5614
                     /gene="rplP"
                     /locus_tag="VGB76_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY0099007.1"
                     /translation="MLMPKKVKHRKQMRGRMTGTATRGAEISFGEYGLKCLEPAWITD
                     RQIEAARIAMTRHIKRGGKIWIRMFPDKPVTKKPAETRMGKGKGAPEYWVAVVKPGRI
                     LYEIEGVDEPTAREAMRLAAQKLPIKTKFVTRAEQEGGAV"
     gene            5620..5841
                     /gene="rpmC"
                     /locus_tag="VGB76_08650"
     CDS             5620..5841
                     /gene="rpmC"
                     /locus_tag="VGB76_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY0099008.1"
                     /translation="MKRHEELEKLRGMSDEDLREEAQRIKESLFRLNFKLALGEMDAV
                     KRLREEKRTLARLQTLITERQNEQQAAGK"
     gene            6138..6359
                     /gene="rpsQ"
                     /locus_tag="VGB76_08655"
     CDS             6138..6359
                     /gene="rpsQ"
                     /locus_tag="VGB76_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY0099009.1"
                     /translation="MVASDKMTNTIVVRVDRLIKHPKYRRYVRRTSKFMAHDETGAAI
                     GDKVRIVETRPLSARKRWRVVEIVQKAEK"
     gene            6462..6833
                     /gene="rplN"
                     /locus_tag="VGB76_08660"
     CDS             6462..6833
                     /gene="rplN"
                     /locus_tag="VGB76_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY0099010.1"
                     /translation="MAIQMQTSLEVADNSGARRVEMIMSIGGSTGRHAVLGDKIKVTV
                     KEAAPDGAVKKGTVHNAVIVRTRKEVRRKDGTYIRFDQNAAVLIKDDNTPIGTRVFGP
                     VARELRDLNFMKIVSLAPEVI"
     gene            6838..7179
                     /gene="rplX"
                     /locus_tag="VGB76_08665"
     CDS             6838..7179
                     /gene="rplX"
                     /locus_tag="VGB76_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY0099011.1"
                     /translation="MAATGIKRAKIRKGDQVIVIAGRDNGKRGRVLEVLPAKNRVKVE
                     GVAIMKRHQKANPQNNRGGGIIDKEAYIDISNVQLIDPESGKPTRVRAGESSEGARQR
                     IATKSGRVIEK"
     gene            7264..7806
                     /gene="rplE"
                     /locus_tag="VGB76_08670"
     CDS             7264..7806
                     /gene="rplE"
                     /locus_tag="VGB76_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY0099012.1"
                     /translation="MAARLKERYQREIAPALAKEFNITNPMATPRLEKIVINMGLGEA
                     IGNSKIIDTAADELSSIAGQKPVTTKAKKSIASFKLREGMPIGSMVTLRGDRMYEFLD
                     RFVSVALPRVRDFRGVSPKAFDGRGNYTLGIRDQLIFPEIDFNKVDKTRGMNISIVTT
                     AKNDEQARALLKALGMPFRQ"
     gene            7888..8073
                     /locus_tag="VGB76_08675"
     CDS             7888..8073
                     /locus_tag="VGB76_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY0099013.1"
                     /translation="MAKKSLIAKANRTPKFSSRAYTRCKRCGRPRGYLRKFQLCRICF
                     RELALQGQIPGVVKSSW"
     gene            8160..8552
                     /gene="rpsH"
                     /locus_tag="VGB76_08680"
     CDS             8160..8552
                     /gene="rpsH"
                     /locus_tag="VGB76_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY0099014.1"
                     /translation="MTDPIADMLARMRNAISAKHSRVDIPASKLKLEIARILKEEGYI
                     NNFVIKGEGIKRTIRIFLRYDARGTSSISHLSRVSRPGRRVYMGSGEIPKVLGGYGVN
                     IVSTSRGLMSGKRARRENVGGEVLAQIY"
     gene            8637..9197
                     /gene="rplF"
                     /locus_tag="VGB76_08685"
     CDS             8637..9197
                     /gene="rplF"
                     /locus_tag="VGB76_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY0099015.1"
                     /translation="MSRIGKKVITVPAGVTVTVGERELTVKGPKGTLTTPIPEGISFQ
                     LEGDQLQAIRQTDELAAQHGLARALANNAIVGVTEGFTKQMDVVGVGYKADVQAKRVV
                     FSLGYSHPIEFPLPEGIEVRGERVPAKTSIQQYQLTLTLSGIDKQKLGQVAAEMHSLR
                     KPDPYKGKGVRYADVVLKLRPGKTGK"
     gene            9278..9628
                     /gene="rplR"
                     /locus_tag="VGB76_08690"
                     /pseudo
     CDS             9278..9628
                     /gene="rplR"
                     /locus_tag="VGB76_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
     gene            9703..10209
                     /gene="rpsE"
                     /locus_tag="VGB76_08695"
     CDS             9703..10209
                     /gene="rpsE"
                     /locus_tag="VGB76_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY0099016.1"
                     /translation="MRQPRQRISAQGLDLKDQVISINRVTKVVKGGKNLSFAALVVIG
                     DESGHVGFGSGKAREVPLAIKKAIESAKKNLIRVPLINNTLPHQLIGKYGAGRVMMKP
                     ASEGTGVIAGGAVRAIMQAVGVHDVRTKVLGSTNPHNVVRATFDGLLNMKDPLELARL
                     RGKQVEEL"
     gene            10299..10508
                     /gene="rpmD"
                     /locus_tag="VGB76_08700"
     CDS             10299..10508
                     /gene="rpmD"
                     /locus_tag="VGB76_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY0099017.1"
                     /translation="MAEQKQEQASGGGTIRVQYYRSFIAFPKTQKEIVRSLGLTKLNQ
                     IVERPDNASMRGVVAKVPHLLRIIE"
     gene            10592..11068
                     /gene="rplO"
                     /locus_tag="VGB76_08705"
     CDS             10592..11068
                     /gene="rplO"
                     /locus_tag="VGB76_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY0099018.1"
                     /translation="MAIGLNNLHAPAGSTHKKKRVGRGPGSGLGKTAGRGNKGQKSRS
                     GYSAKIGFEGGQMPLQRRLPKRGFTNIFKKQWIEVSLSSLENSFEANEEVTPELMHER
                     GVIAKGKRDVVVLGTGEISKALRVAAHRFTKSAREKIEGAGGAVTVIEKQSGNAPQ"
     gene            11362..12780
                     /gene="secY"
                     /locus_tag="VGB76_08710"
     CDS             11362..12780
                     /gene="secY"
                     /locus_tag="VGB76_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY0099019.1"
                     /translation="MEKFFAAVRNMFQVPDLRKRIAFTLGLLAVYRLGAHVTAPGINK
                     DQLARVWNEVGNTLLGVLDLFSGGNFQTISVFALGVTPYITASIILQLMTTVFSQLKK
                     LQEEGEAGRQKINQYTRYLTVVLACFQTSIVARWLQANGVGEPGWGFLLTTVLTLTTG
                     TIFVMWLGEQITERGIGNGISLLIFAGIIIGLPSGMMQVLGKLRSGVAMDVVGVIALV
                     VALVLLIAFIVFVEQARRKVPISYAKRHVGRQLVGGQQTVMPLKINMAGVIPVIFASS
                     MLAFPQTILQFIQYDPAQPESWRSRVHLFFQSFHGGDPYYELVFISLIVIFTFFYITI
                     IFNVEEVADNLRKHGGFIPGIRPGRNTAEFLNTILTRLTTVGAIYLALVALLPQIMLS
                     GFNVGRLPFVGSGLEDFFSRTPGLGWIPSGMGYKFYFGGTSLLILVGVAMDTVAQIEA
                     QLVMRNYEGFLGAGGRLRGRRT"
     gene            12878..13534
                     /locus_tag="VGB76_08715"
     CDS             12878..13534
                     /locus_tag="VGB76_08715"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY0099020.1"
                     /translation="MSKIVVLMGAPGAGKGTQARLLQERLGLPQISTGDMFRELGKAE
                     TPLAAEVRAVQAAGKLVSDDLVIRVVHERTGRADCKHGYILDGFPRTTAQAAMLEKLA
                     VEQGNKIQAVLVDVPFELLEQRATGRRSCPVCGEIYNVYFKPPQREGFCDLHPETPLT
                     HRADDTAEKIRVRLATYEEQTRPLLDYYEASHRLEGVDGTRDPEAIYADIEKIVTGEK
                     "
     gene            13605..14417
                     /gene="map"
                     /locus_tag="VGB76_08720"
     CDS             13605..14417
                     /gene="map"
                     /locus_tag="VGB76_08720"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY0099021.1"
                     /translation="MIIGKSKKELEKMRAAGRLAGQVREALRRMARAGVTTLDLDRAA
                     ERMIRDAGALPTFKGYHGYPYSICASVNEQVVHGFPSDYVLCEGDIISIDVGATLDGF
                     VGDTATTVPVGNVSEDKLRLIRVAEESLERAIEQCRHGRHLGDIGWAVQELAEAEGYT
                     LVRDYGGHGIGRKMHEDPHIPNYGRPGTGSKIKNGYVFAVEPMLNMGTHHTKTLSDGW
                     TVVTVDGQPSAHVEHTIAITEEGPQVFTRMPEELERLTAAKAEAGVAVEARV"
     gene            14567..14680
                     /gene="rpmJ"
                     /locus_tag="VGB76_08725"
     CDS             14567..14680
                     /gene="rpmJ"
                     /locus_tag="VGB76_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY0099022.1"
                     /translation="MKVRASVKKMCDNCKVIHRKGVVRVICTNPKHKQRQG"
     gene            14739..15122
                     /gene="rpsM"
                     /locus_tag="VGB76_08730"
     CDS             14739..15122
                     /gene="rpsM"
                     /locus_tag="VGB76_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY0099023.1"
                     /translation="MARVVGVDLPVNKRVEIGLTYIYGIGRSRSKSILQEADINLDTH
                     VRDLDEEQLGRIRAIIEGQGDVEGDLRKRVQMDIKRLMDIGAYRGLRHRRGLPVRGQR
                     THTNARTRKGPRRATIAKKKAPGKK"
     gene            15244..15651
                     /gene="rpsK"
                     /locus_tag="VGB76_08735"
     CDS             15244..15651
                     /gene="rpsK"
                     /locus_tag="VGB76_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY0099024.1"
                     /translation="MAKATAAGGKKKTFRKKEKKNIPNGIAFIAASFNNTMISITDLE
                     GNLIAQSSSGARGFRGSRKGTPFAAQQAAYEAAQKAKDAGMQQCEVRVKGPGGGRESA
                     IRAINNAGIRVTLIRDTTPIPHNGCRPPKRRRV"
     gene            15843..>16440
                     /gene="rpsD"
                     /locus_tag="VGB76_08740"
     CDS             15843..>16440
                     /gene="rpsD"
                     /locus_tag="VGB76_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY0099025.1"
                     /translation="MARYRAAVCRLCRREGAKLFLKGDKCYKPSCPIEARGTQPPGQH
                     GNSPRRGKALVGYGQQLREKQKVKRIYFVLEGQFRNYFEKAVRQKGITGENLLVLLER
                     RLDNAVYRAGFSTSRRQARQLVNHGHIEVNGRKVDIPSFQVKAGDVVTVRETSRKNAH
                     IEGAWQTAAGRGRPSWLAPTDGAEMGARVASLPTRDDID"
BASE COUNT         3805 a         4551 c         5125 g         2959 t
ORIGIN      
        1 ggtcggggct ggtaccatct ccgaaatcgt ggaataagtt tgatcaaccg atgaccggcc
       61 atgagcgaag aacgtagtgc tggcacaagg ttgattgcaa aaagctggta gctaaaagct
      121 aataattatg ctgaacgaaa agatcagaat cagactcaag gcttacgatc atcgagtgct
      181 ggatcaatcc acctccgaga tcgtggagac tgcgaagcgc acaggggcgc gcgtggcggg
      241 gccaatcccg cttcccacct ccaaaaacaa gtggacggtc ttgcgttcgc cgcacgtcga
      301 taaaaagtcg cgcgagcagt tcgagatcag gacgcacaag cgtctgatgg acattctcga
      361 cccgaccccg cagaccgtgg atgcgctgat gaaactcgac ttgcccgcag gcgtggatgt
      421 cgagatcaaa gcgttcggca gagacagata agagcggtga caagtggcaa gtgacaggtg
      481 acaagatgaa aaacttgttc tttcctgtca cctgtcacct gtcacctgtc accgaggtat
      541 tgtgatgatt aacggaatca tcggtaagaa agttggcatg acgcagttgt tcgcacccga
      601 tggcacggtc actccggtga cggtcatcaa ggcgggtcct tgcgtcgtgg tgcagaccaa
      661 atcggcggcg ggcaaagatg gttacgacgc cgtgcagatc gggctggtcg aagaacgccc
      721 ggtgaagttg aagaacgtca acaagccgat gcgcggccac tttgaaaaga cgggcgcagg
      781 cacgccgcca acgcgcgtct tgaaagagtt tcgccttgaa ggcgatgcgg gcgtcagcgt
      841 gggcgacacg gttctggtcg gcgagcagtt tgccgacggc gacacgattg aagtgatcgg
      901 caagagcaag ggtcggggtt tcgcgggcac ggtgaagcgt cataacttca atcgtggtcc
      961 tgaatcgcac ggctcgatga acgtgcgcgc gccgggttca atcggccagt cggcctatcc
     1021 ttcgcgtgtc atcaagggca cgcgctcgtc cggccacatg ggcgacgtgc gcatcaccgt
     1081 taaaggtttg acggtcgcac acgtggatgc cgaaaacaat ttgttgatga ttcgcggcgc
     1141 ggtgcccggc gcaaacggca cggtcgtcat cgtcaaaaag tctgtcagac agaagaagaa
     1201 atgatgaacg atgaacgcgg aacgatgaac ggaaagcagt tatcttaact tcatccttcc
     1261 ccgttcctac ttcatcgtta tggagtgtgt gtttatgcct accgtgaagg tgcgcaattt
     1321 aaagaacgaa gagatcgccg acctcgaact gtccgacgcc gtgttcggcg cggagtataa
     1381 cgagggcttg gtgcacgccg ccgtcaaggg ctacttggcg aaggcgcgtg cgggcacggt
     1441 cggcacgaag acgcgcggag acgtgtccgg ttcgggcaag aaactctgga agcagaaggg
     1501 caccggacgc gcacgcatcg cgagcatccg ttcgcccctg tggaaaggcg gcggcaacgt
     1561 gcatggaccg caggcgaagg attggtcgca caatctgccg aagaagatgc gacgcggcgc
     1621 gttgcgctcg gccttgtcgg agcgtctgcg cgaaggcaac gtcctgatcc tcgacgcctt
     1681 tacgctcggc caggcgaaga cgaaagattt catcagcgcg ctcggcacgc tcggcctcga
     1741 aggcaagact ctgatcgtgg attcgcacga taacgacaac ctgctgctcg cttcgcgcaa
     1801 cgtcaagcgt gcgaagatca cgacgagccg cagcctgaac atctacgatc tgctctatca
     1861 cgaaaaactc gtgctctcgc gcacggcggc ggaagagttg ggcgacatgc tcgacccgaa
     1921 gcgacgcacc ggcgcgcacg acgaagagac ggacgaaggc ggcacgacgc ctgacgccgg
     1981 agcagcgaat acgacagagg aggcggcgta aaagatgcga acagtgtggg acatcatcaa
     2041 gtcgcccgtg atcacggaga aggctctcgt cgcgaaagaa gagacgcagg acggcaagca
     2101 actcttgacc ttccgcgtgg ataaaaatgc cacgaagccc gagatcaagt cggccatcga
     2161 gcgaattttc gacgtgaaag tcgcttccgt gcgcacgatg aattacatgg gcaagaaggc
     2221 gcggcgcggt cgcttcgaag ggcgcaagcc ttcgtggaag aaagcctacg tcacgctcaa
     2281 agaaggcgaa aagccgctcg attacggcga agcgatctaa aagcagtgac aagctgcaag
     2341 tgacgtgtga caagttgaaa cttgttcctg cccgtcacca gtcgcctgtc acccgtcact
     2401 taaaagatta tgggcatcaa gaaacttaca ccgacatcac cggcgcgtcg ttaccagacg
     2461 tatctgacgc gcgatgagtt gacgaagggc gcggagcctg agaagtctct gctggagaag
     2521 aagacgcgca tctccgggcg caataacgac ggccacatca ccgtgcggcg gcgcggcggc
     2581 ggcgcgaagc gtttttatcg catcatcgat ttcaaacgtg acaagacggg cgtgcccggt
     2641 cgcgtcacgc aactcgaata cgatccgaac cgctcggcgc acatcgcgct catcacctat
     2701 ctcgacggcg agaagcgtta catcctcgcg cccgccggga ttgaagtcgg gaagacgatc
     2761 atgagcggtc ccgaagcgga cattctggcg ggcaacgccc tgccgctcaa aaacattccg
     2821 ctcggcacgc agattcacaa catcgaactg cgccccggca aaggcgggca gatggcgcgc
     2881 tcagccggtg cgttcgcgca actcgtcgcc aaagaaggcg actacgcgca actgcgtatg
     2941 ccttcgggcg aagtgcggcg cgtgcccgtc gtctgcatgg cgaccgtcgg gcaggtgggc
     3001 aacgtcgagc atgaaaacgt ctcgttcggt aaggccggac gctcgcgctg gaagggcatc
     3061 cgcccgcacg tgcgcggcgt ggcgatgaac cccgtggatc acccgcacgg cggcggcgag
     3121 ggcaagacct ccggcggacg ccatcccgta acgccctggg gacaacccac gcgcggctac
     3181 aagacgcgtt caaacaagcg cacgcagaag atgatcgtgc gcgatagaag agttaaataa
     3241 gggatgaagg atgagggatg agggatgaat aaggcgggat gataaagcga cagcttttct
     3301 gattcatccc tccgtcctcc gccctcgtcc cttaaaagtg gagcgaagcg aaacgttatg
     3361 gcacgttcag ttaaaaaagg tccgtttatt gacgattacc tgttgaagat ggtggcgcgg
     3421 attcgcgaga gcggcgcacg tccgcccgcg atcaagacct ggtcgcggcg ttcgacgatc
     3481 acgccggatt tcgtcggcct gaccttcgcc gtgcataacg gcaaacgcca tctgcccgtg
     3541 tacgtcacgg agaacatggt cggacacaaa ctcggcgaat ttgcgccgac gcgcaccttc
     3601 aaaggccact cgggaacgaa ggccgagaag ggcggaaaag tagcgagata gcagtggcaa
     3661 gtgacaagtg acgagtgaca agttggaaag caacttcttg tctctggttc gtcactcgtc
     3721 actcgtcact cgtcactgag gttaagagat ggacgcaaga gcgagtgcaa aatatctgcg
     3781 cgggtcgccg caaaaagcgc gactcgtggt tgacatgatc cgtggcaaaa acgtcaacga
     3841 agcgttggcc gtgttgcagt acacgaacaa acgcgcggcg gacggcatcg agaagtgcct
     3901 gcgttcggct gtggcaaacg cgaccgaagc ggcggagaag gcgaacatcg ccgtcgatcc
     3961 ggacgatctg tgggtcaagg cgtgtttcat cgacatgggg ccgacgaaga accgacgccg
     4021 catgcgcccc gcgccgcaag ggcgcgctta caaagaacgg cgacactttc atcacgtcac
     4081 cgtgctgttg tcgagcgacg aacaagaggc tctgaagggc aggcgtccgt cgaaagcggg
     4141 caagccgcag acgaccgccg cgagcaagac gcgcaagaaa accgacggca ccgcaactgc
     4201 cgcgaccaag accgcgacca aacgcgcggc ctcgaagaaa cctgagacga ctgctgttga
     4261 aacgcccgcc gccgctgagt cggcaagtgg cgacacggca accgcagaat agttaagttt
     4321 gaacggagaa taaatgggtc agaaagttca tccatacggc ttccgcctcg gctataacaa
     4381 agactggcac tcgcactggt ttgcgaagaa acagtacggc gaatacctgc tcgaagacct
     4441 gaagctcaag gcgacgctca aggcgcgttt caacggcgcg ggcgtctcgc acgtagacgt
     4501 ggagcgtgcg gccaatcgct tgaagatcat catctacact tcgcgtcccg gcatcatcat
     4561 cgggcgcaaa ggcgcggaga tcgacaagct caaggccgac atcgcctcgc gcacgggacg
     4621 cgaagtgctc gtctcgattc aagagattca caagccggaa ctcaacgcgc agttgcaggc
     4681 cgagaagatc gccgtgcagc ttgagaaacg catcgccttc cgtcgcgcca tgcgcaagac
     4741 cgtggaagag acgcttcgct tcggcgggca gggcatcaag gtcaaggtcg gcggtcgtct
     4801 gaacggcgcg gagatcgcgc gcagcgagtg gtacttgcag gggcgtctgc cgctgcacac
     4861 cctgcgcgcc gacattgatt acggtttcgc gcaggcgcac acgacgttcg gcgtgatcgg
     4921 cgtcaaggtc tggatttaca ggggcgagat tctcgacccg aaagcggcga tggcgcgcgg
     4981 cacagcgacc gacccgatca acgaaccgcg cggcgagcgt ggcgaacgcg agcgtcgccc
     5041 gcgcacacgg cgcgaacgcg aaggcggcga caatcgcggc gaccgtggag atcgcggaga
     5101 tcgtggtcgt ccgcgcggtg agcggcaata gttaaaagtt tattttgtga tctcaggtta
     5161 agagatcgac aggggtgcat tcatcatgtt gatgccgaaa aaagttaagc accggaagca
     5221 gatgcgcggg cgcatgacgg ggacggccac gcgcggcgcg gagatcagct tcggcgagta
     5281 cggcctgaaa tgtttggagc cggcgtggat cacagaccgc cagatcgagg cggctcgtat
     5341 cgccatgacg cgccacatca agcgcggcgg gaaaatctgg attcgcatgt tccccgataa
     5401 gccggttacg aagaagcccg ccgaaacgcg catgggcaaa ggcaagggcg cgccggaata
     5461 ctgggtggcg gtggttaaac cgggtcgcat actttatgag atcgaaggcg tggacgagcc
     5521 gacggcgcgc gaggcgatgc gtctcgccgc gcagaagctt ccgatcaaga cgaagtttgt
     5581 gacgcgcgcc gagcaagaag gaggcgcagt ctagtagtta tgaaacgaca cgaagagttg
     5641 gaaaaattgc gcggcatgtc ggacgaagac ctgcgggaag aagcacagcg catcaaagag
     5701 tctctgtttc gtctcaactt caaactcgcg ctcggcgaaa tggacgcggt caagcgtctg
     5761 cgcgaggaga agcgcacgct ggcgcgtttg cagacgctca tcacggagcg gcagaacgaa
     5821 cagcaggcgg cgggaaaata gattagtgaa tcgtagatcg tgaatagaaa aaaactgttt
     5881 tagtctattg acgattgacg attcacgact aacgaggatt tgtgatggca gaagaaaatc
     5941 aggaacagac ggctgctgct ggcggggacg cgccaaagga agagagcgcg agcgcgaccg
     6001 acgagacgcg cgtggcggaa gcgaccgact cgggcgaggc actcgcgcac gaagttgcgc
     6061 cgaacgtaga ctcgcaggcc ggtaacgcct cgggcacgga agcgggcaag cgcgggcgtc
     6121 gcgcagagaa ggtcggcatc gtcgcctcgg acaagatgac gaacacgatt gtcgtccgcg
     6181 tcgatcgctt gatcaagcat ccgaagtatc gccgctacgt gcgccgcacg tcgaagttca
     6241 tggcgcacga cgagacgggc gcggccatcg gcgacaaggt gcgcatcgtc gagacacgtc
     6301 cgctctcggc cagaaaacgc tggcgcgtcg tagagatcgt gcagaaagct gagaaatagt
     6361 gacaagtgac gggtgacaag cgacaagtcg aagcaaagca agccaaatgc tttaactcgt
     6421 cacttgtcac ttacaactcg tcactgcgaa cggagtgagt tatggcaatt caaatgcaga
     6481 catcgctgga ggtggccgat aattcgggcg cgcggcgtgt cgagatgatt atgagcatcg
     6541 gcggttcgac ggggcggcac gctgtcctcg gcgataagat caaggtgacc gtcaaagagg
     6601 ctgcgccgga cggcgcggtc aaaaaaggga cggtgcacaa cgccgtgatc gtgcgcacgc
     6661 gcaaagaggt gcgccgcaaa gacggcactt atatccgctt cgaccagaac gcggcggtgc
     6721 tcatcaaaga cgacaacacg cccatcggca cgcgcgtctt cggccccgtc gcacgcgaac
     6781 tgcgcgacct gaattttatg aagatcgttt cgctcgcgcc ggaggtgatt taagacaatg
     6841 gcagccacag gaatcaaacg cgccaagatc agaaaaggcg atcaggtgat cgtcatcgcc
     6901 ggacgcgaca atggcaagcg cggtcgcgtg ctcgaagtgt tgcccgcgaa gaaccgcgtc
     6961 aaggttgaag gcgtggcgat catgaagagg catcagaaag ccaacccgca aaacaatcgt
     7021 ggcggcggca tcatcgataa agaggcttac atcgacatct cgaacgtcca gttgatcgac
     7081 ccggaatcgg gcaagccgac gcgcgtgcgc gcgggcgaga gcagcgaagg cgcgcgccag
     7141 cgcatcgcga cgaaatcggg acgcgtgatc gagaagtagt ttttgcgtta actgaagagc
     7201 agtctgagtc tggaaacgcc ggtcacgcgg gcgataccca gacgaaaggt agagagagag
     7261 aaaatggcag ccagattgaa agagcgttac cagcgagaga tcgcgcccgc gctcgccaag
     7321 gagttcaaca tcacgaaccc gatggcgacg ccgcgactcg aaaagatcgt catcaacatg
     7381 ggactcggag aggccatcgg caactccaag atcatcgaca cggcggcaga cgaactctcg
     7441 tccatcgcgg ggcagaagcc cgtcacgacg aaagccaaga agtccatcgc gtcgttcaaa
     7501 ctgcgcgagg gcatgcccat cggctcgatg gtcacgctgc gcggcgaccg catgtacgag
     7561 tttctcgacc gcttcgtctc cgtcgccctg ccgcgcgtgc gcgattttcg cggcgtctcg
     7621 ccgaaggcgt tcgacgggcg cggcaactac acgctcggca tccgcgacca gttgatcttc
     7681 cccgaaattg atttcaacaa agtggacaaa acgcgcggga tgaacatctc gattgtcacc
     7741 accgcgaaaa acgacgagca ggcacgcgcg ctcctcaagg cgctcggcat gccgttcaga
     7801 caatagagca gtgacaggtg gcaggtgaca agttaaaact aaaaacttgt ctgcaactcg
     7861 tcactcgtca ctcgtcactg aggtcatatg gcaaagaaat ctctaatcgc gaaggcgaac
     7921 cggaccccga agttttcatc gcgcgcctac acgcgctgca agcgttgcgg caggccgcga
     7981 ggctatttgc gcaagttcca actctgtcgc atctgcttcc gcgaactcgc gctgcaaggg
     8041 cagattccgg gcgtcgtcaa gtcgagttgg taattattag ataattgacc gtcagcagtc
     8101 agccgctcgc gtgagatgag aggttgaaac ctggcggcgt aaactggagc tgtagagtaa
     8161 tgactgatcc tattgccgac atgctggcgc ggatgcgaaa cgcaatctct gcgaagcact
     8221 cgcgggtaga catcccggcg tcgaaattga aattggagat cgcccgcatt ctcaaagaag
     8281 agggctacat caacaacttc gtcatcaagg gcgaaggcat caagcgcacg atccgcatct
     8341 tcctgcgcta cgatgcgcgc ggcacttcgt ccatctcgca cctctcgcgc gtgtcgcgcc
     8401 ccggcaggcg cgtctacatg ggttcgggcg aaatcccgaa ggttctgggc ggctacggcg
     8461 tcaatatcgt ttcgacttcg cgcggcctga tgagcggcaa acgcgcccgc cgtgaaaacg
     8521 tcggcggcga agttctggca caaatctact aagggaggaa ggatgaggga tgagggatga
     8581 attaaaaaca tctctgattc atccctcatc cctcagccct catccttgag gttgttatgt
     8641 ctcgtattgg aaagaaagtt atcaccgtgc ctgcgggcgt gaccgtgacg gtcggcgagc
     8701 gtgagttgac ggttaaagga ccgaagggaa ctttgacgac gccgattccc gagggcatca
     8761 gcttccagtt ggaaggcgac cagttgcagg cgattcgcca gacggatgaa ctggcggcgc
     8821 agcacgggct ggcgcgcgcg ctggcgaaca atgccatcgt cggcgtgacc gaaggcttta
     8881 cgaaacagat ggacgtggtc ggcgtcggtt acaaggcaga cgtgcaggcg aagcgggtcg
     8941 tcttctcgct cggctattcg cacccgattg agtttccgct gcccgaaggc atcgaagttc
     9001 gtggcgagcg cgttccggcg aagacttcga ttcaacagta ccagttgaca ttgacgctct
     9061 ccggcatcga caagcagaaa ctcggacagg tcgccgccga gatgcactcc ctgcgcaagc
     9121 ccgatcccta caagggcaaa ggcgtgcgct atgccgacgt ggtcttgaaa ctgcgtcccg
     9181 gcaaaactgg aaaataagta tgaagtagga acgatgagcg atgaagtgaa agcgacatct
     9241 tttattcatc gttccgcgtt catcgttgga gtttacaatg gcacagaaag atagagcaga
     9301 agttcgcagg gcggtgcaca agcgcattcg ccgcaaggtg tcgggcagcg cagtgcgccc
     9361 gcgcctgacg atctaccgca gcctgaacca catctacgcg cagatcatag acgacgagca
     9421 gggacgcacc atcgccgccg cttcgacgac cgagaaagac ctgcgcggcg cgacgggcgg
     9481 aaacattgat gccgccgtgc gcgtgggtcg ggcgattgcc gagcgcgcct tgtcgaacgg
     9541 catcgagagc gtcgtcttcg atcgcggcgg gtatcgctat cacggtcgcg tcaaggctct
     9601 ggccgatgcc gcgcgtgagg ccggattgaa caaagaagag gccgccgccg cagcagcaga
     9661 acaggggcag gccgcagaag cagcagcagg acaggagcag taatgcgaca accgagacag
     9721 agaatttcag cgcagggact tgatctcaaa gatcaggtga tctcaatcaa ccgcgtgacc
     9781 aaagtggtca agggcggtaa gaacttgagt ttcgccgcgc tcgtcgtcat cggcgacgaa
     9841 tccggccacg tcggcttcgg cagcggcaag gcgcgcgaag tgcccctcgc catcaagaag
     9901 gccatcgagt cggcgaagaa gaatttgatc cgcgtgccgc tcatcaacaa cacgctgccg
     9961 catcagttga tcggtaaata cggcgcgggt cgggtgatga tgaaaccggc ttcggaaggc
    10021 acgggcgtga tcgcgggcgg cgcggtgcgc gccatcatgc aggcggtcgg cgtccacgac
    10081 gtgcgcacta aggtgctcgg ctcaaccaac ccgcacaacg tcgtgcgtgc gaccttcgac
    10141 gggctgttga acatgaaaga cccgttggaa ctggcgcgcc tgcgcggcaa gcaggtggaa
    10201 gaactataaa gacgtagatc gtgaatcgtg aagagaggaa aacagttctt atctatttac
    10261 gattcttgat tctcgattta cgagattgag gtttagaaat ggcagagcag aaacaagagc
    10321 aggcgagcgg cggcggtacg atccgcgtcc agtattaccg gagcttcatc gctttcccga
    10381 agacgcagaa agagatcgtc agaagcctcg gcctgacgaa gttgaaccag atcgtcgagc
    10441 ggcctgacaa tgcctcgatg cgcggcgtcg tcgccaaagt gccgcacctg ttgcgaatta
    10501 ttgagtagga gaagattggt gaatcgttaa tcgtgaatag aaggaactga gttttacctg
    10561 tttacgattg acgattcacg aggtattcat catggctatt ggtcttaata atctacacgc
    10621 tccagccggt tcgacgcata agaagaagcg cgtcgggcgc ggtcccggtt cgggtctggg
    10681 caagacggcg gggcgcggta acaaaggtca gaagtcgcgt tcgggctact cggccaagat
    10741 cggttttgaa ggcggacaga tgcccttgca gcgtcgcctg ccgaaacgcg gtttcaccaa
    10801 catttttaag aagcagtgga tcgaggtcag cctctcgtcg ctcgaaaaca gttttgaggc
    10861 gaatgaagag gtgacgccgg agttgatgca cgagcgcggc gtgatcgcca aaggcaagcg
    10921 cgacgtagtg gtgctcggca cgggcgagat ttcgaaagcc ctgcgcgtgg cggcgcatcg
    10981 cttcaccaaa agcgcgcgtg agaagatcga aggggcgggc ggcgccgtca ctgtgatcga
    11041 aaagcagtcc ggtaacgctc cgcagtaatt ttagcgacgg ctctggttgg tcgtcagcga
    11101 ttttcgcggc atcgagcaaa tgtcaaagag ccagaagacg cagagagcgt gtgcgccgga
    11161 agcttcggtg tctgccctgc gtctttagtc gctcttgaga agctgtcccg ccgtcgtcgg
    11221 atttcgattg ctgcccggac gcttgactca aatgtcgcag cgacctggcc tgacaccgga
    11281 cgcgtctccg gttggctgcc gcaccgtttc tgttttaagc tgatagccga tagctcaaag
    11341 cctctcgaaa gaagcactct tatggaaaag tttttcgccg ccgttcgcaa catgtttcaa
    11401 gtgccggatt tgcgcaaacg catcgcgttc acgctcggcc tgctggcggt ctatcgcctc
    11461 ggcgcacacg tcaccgctcc cggcatcaat aaagatcaac tcgcgcgcgt ctggaatgag
    11521 gttggcaaca ccctgctcgg cgtgctcgat cttttctccg gcggaaattt ccagaccatc
    11581 tcggtcttcg cgctcggcgt gacgccctac atcaccgcct cgatcatcct gcaactgatg
    11641 acgaccgttt tctcgcagtt gaagaaattg caggaagagg gtgaggccgg gcggcagaag
    11701 atcaaccagt acacgcgcta tctgacggtc gtgctcgcgt gctttcaaac ttccatcgtc
    11761 gcgcgctggc tgcaagccaa cggcgtcggc gaacccggtt ggggcttcct gctcaccacg
    11821 gtgctcactt taacgacggg tacgatcttc gtgatgtggc tcggcgaaca gatcacagag
    11881 cgcggcatcg gcaacggtat ctcgctcctg atcttcgcgg gcatcatcat cggactcccg
    11941 agcggcatga tgcaggtgct cggaaaactt cgcagcggcg tggcgatgga tgtcgtcggc
    12001 gtaatcgcgc tcgtcgtcgc gctggtcttg ctcatcgcct tcatcgtctt cgtcgaacag
    12061 gcgcggcgca aagttccgat cagttacgcc aagcggcacg tcgggcgtca actcgtgggc
    12121 gggcagcaaa ccgtgatgcc tttgaagatc aacatggcgg gcgttatccc cgtcatcttc
    12181 gcctcttcga tgctggcctt cccgcagacg atcttgcagt tcatccagta cgatccggcg
    12241 cagcccgaat cctggcgcag ccgcgtgcac ctgttcttcc aatcgtttca cggcggcgac
    12301 ccgtattacg agttggtctt catctcgctc atcgtcatct tcaccttctt ctacatcacg
    12361 atcattttta acgtggaaga ggtggccgac aatttgcgca aacatggcgg cttcatcccc
    12421 ggcatccgac ccggcaggaa caccgccgaa tttttgaaca cgatcctgac gcggcttacg
    12481 accgtcggcg cgatctatct tgcgctcgtc gcgctcctgc cgcagatcat gttgagcggc
    12541 ttcaacgtcg ggcgtctgcc cttcgtcggg tcgggcttgg aagacttctt cagccgcacg
    12601 ccgggcttgg gctggattcc gagcggcatg ggttataaat tctatttcgg cggcacgagc
    12661 ctgctgattc tcgtcggcgt cgcgatggac accgtagcgc agatcgaagc gcaactcgtc
    12721 atgcgcaact acgaaggctt cctcggagcg ggaggccgcc tgcgcggacg cagaacatag
    12781 atttaatgat gaagtaggaa cgtggaatga tgaataaaaa tctactgctt taatttcatc
    12841 attcaacgtt cctacttcat cgttcatctt ctcgcttatg tcaaagatcg tcgtcttaat
    12901 gggcgcgccg ggcgcgggca aagggacgca ggcgcgtctc ctgcaagagc gtctgggctt
    12961 gccgcaaatt tcgacgggcg acatgtttcg tgaactcggc aaagcagaga cgccgctcgc
    13021 cgcagaggtg cgcgccgtgc aggccgcggg caaactcgtc tcggacgatc tcgtcatccg
    13081 cgtcgtgcac gaacggacgg gacgcgcaga ctgcaagcac ggttatattc tcgacggctt
    13141 tccgcgcacg acggcgcagg ccgcgatgct ggaaaaactg gccgtcgagc agggcaacaa
    13201 gattcaggcc gtgctcgtcg atgtgccctt tgaacttttg gaacaacgcg cgacagggcg
    13261 tcgcagttgt cccgtgtgcg gtgagattta caacgtctac ttcaagccgc cgcaacgcga
    13321 aggcttctgc gacctgcacc ccgaaacgcc tttgacgcat cgcgccgacg atacagcgga
    13381 aaaaattcgc gtccggctcg cgacctacga agagcagacg cgcccgctcc tggactacta
    13441 cgaagcgtcg catcgccttg agggtgttga cggcacgcgc gacccggagg cgatctacgc
    13501 cgacattgaa aagatagtga cgggtgagaa gtgacgggtg acaagttaaa accaaaaact
    13561 tgtctttatc tcgtcacttc tcactcgtca cttgtcactg tcttatgatt attgggaagt
    13621 cgaaaaaaga gttggagaag atgcgtgcgg cggggcgtct ggcggggcag gtgcgcgagg
    13681 ctctgcggcg catggcgcgt gcgggcgtca caacactcga cctcgaccgc gccgccgagc
    13741 ggatgatccg cgacgcgggc gcgctgccga cgtttaaagg ctatcacggc tatccctact
    13801 ccatctgtgc gtcggtcaac gaacaggtcg tgcacggctt tccttcggat tacgtgctgt
    13861 gcgagggcga cattatttcg attgatgtcg gcgcaacgct ggacggtttt gtcggcgata
    13921 cggcgacgac cgtgcccgtg gggaatgtca gcgaagataa actccgtctg attcgcgtcg
    13981 cggaggagag tctggaacgc gccatcgagc agtgccgtca cggcaggcat ctgggcgaca
    14041 tcggttgggc ggtgcaggaa ttggccgaag cggaaggcta cacgctcgtg cgcgattacg
    14101 gcggccacgg catcgggcgg aaaatgcacg aagacccgca catcccgaat tacgggaggc
    14161 cgggaaccgg ctcgaagatc aagaacggct acgttttcgc cgtcgagccg atgctcaaca
    14221 tgggcacgca tcacacgaag acgctctccg acggctggac ggtcgtgaca gttgacgggc
    14281 agccgagcgc acacgtcgaa catacaattg ccatcacgga agaggggccg caggtgttta
    14341 cgcgcatgcc cgaagaactg gagcgtttaa cggctgcgaa agcagaggcc ggggttgccg
    14401 tggaggcgcg tgtgtgagac gcgtgcggaa gcgtctctgg cttggcataa ggtcaatgct
    14461 gtgttaatat ccgcagtttg ccgatactgg catttttaaa tctatcgaaa tgcggtcggg
    14521 tttgagtcag aggccgtgta cttccggctg aaaaggtgga tgcgttatga aagtcagagc
    14581 ttccgtaaaa aagatgtgtg ataactgcaa ggtcattcac cgcaaaggtg tggtgcgcgt
    14641 gatttgcacg aatccgaagc acaaacagcg tcaaggctaa ttgttgattt caaatttgag
    14701 attcacgtct caggtttttt gagagttgga gagaagaaat ggctcgtgtg gttggcgtcg
    14761 atcttccggt aaacaaacgc gtcgagatcg gccttacata tatttacggc atcgggcgtt
    14821 cgcgttcaaa gtcgattctg caagaagcgg acatcaacct cgatacgcac gtgcgcgacc
    14881 tggacgaaga gcaactcggt cgcatccgcg cgatcataga ggggcagggc gacgttgaag
    14941 gcgacttgcg caagcgcgtg cagatggaca tcaaacgttt gatggacatc ggcgcgtatc
    15001 gcgggctgcg tcatcgtcgc gggcttcccg tccgcggcca gcgcacgcac accaatgcgc
    15061 gcacacgcaa aggaccgcgc cgcgccacca tcgccaagaa gaaggcgccc ggcaagaaat
    15121 aggaagccgt cgtccgttag ctattggcag gcagcttttt gtctaatggc gggcggtaca
    15181 ggtttaaatg aaggcggtgg cgcgttgggt tgtgctaatc gctaagagct taagagcgga
    15241 tttatggcaa aagcaacagc agcaggcggc aagaaaaaga cctttcgcaa gaaagagaag
    15301 aagaatattc cgaacgggat cgccttcatc gcagcatcgt tcaacaacac gatgatctcg
    15361 atcaccgatc tggaaggcaa cctcattgcg cagtcgtctt cgggcgcacg cggttttcgc
    15421 ggttcgcgca agggcactcc gttcgccgct cagcaggcgg cctacgaggc ggcgcagaag
    15481 gcgaaagacg cggggatgca gcagtgcgaa gtgcgcgtca aggggccggg cggtggtcgt
    15541 gagtcggcca tccgcgcgat caacaacgcg ggcatccgcg tcaccttgat ccgcgacacg
    15601 acgccgatcc cgcacaacgg ttgccgccca ccgaaacgac gcagagtgta aaagggcagt
    15661 gacaggtggc aagtcacggg tgacaagtta aaacaaatgc ttgtctctgg ctcgtcactt
    15721 gtcactcgtc gcttgtcatt gttttattga cctaaaggat gaagggtcgc cattagcgca
    15781 agggcgcaga gggcggtctg taaacttttc cgtcttaacc gagagactgg aggcagaaag
    15841 aagtggctag gtatagagcc gcggtgtgcc gtttgtgccg ccgcgaaggc gcaaaactat
    15901 ttttgaaggg cgacaagtgc tacaagcctt cctgcccgat tgaagcgcgc ggcacgcagc
    15961 cgcccggaca acacggcaac tcgccgcgtc gcggcaaagc cctcgtcggc tacggccagc
    16021 agttgcgcga gaagcagaaa gtcaaacgca tctacttcgt gctcgaaggg cagtttcgca
    16081 actacttcga gaaagccgtg cgccagaagg gcatcacggg cgaaaacctg ctcgtcctgc
    16141 ttgagcgcag gctcgacaac gcggtctatc gcgccggttt ctccacctcg cggcggcagg
    16201 cgcgccagtt ggtcaatcac ggccatatcg aagtgaacgg gcgcaaagtt gacatccctt
    16261 cttttcaggt gaaagccgga gacgtggtga cggtgcgcga aacgagccgc aagaacgccc
    16321 acatcgaagg cgcgtggcag acggctgccg gacgcggtcg cccctcctgg ctggcgccca
    16381 cggacggcgc ggagatgggc gcgcgcgtgg cgagtttgcc gacgcgcgac gacattgacc
//
LOCUS       DASWNS010000246         9230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_478600, whole genome shotgun sequence.
ACCESSION   DASWNS010000246 DASWNS010000000
VERSION     DASWNS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9230
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478600"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4516)
                     /locus_tag="VGB76_08745"
     CDS             complement(<1..4516)
                     /locus_tag="VGB76_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099026.1"
                     /translation="MLKTAADKFVIAALLLVLAFAPGAGFAQPQQTPADSLISNRAEA
                     IYADEEGNEFSTVSPTITVTVRPVSALVVTPDEAAPSAAVAPQERITRLFRVCNFGNT
                     PDLYTITRAEVSTPATIFSLHFDTDDNGVLSDADTSINLNSTMSPRLAPRACINVLAV
                     VDTNASAPQTQLVIRLDARSNVSMTVNGTVEDTGTIINAVGERARLTNPTNPALPPSK
                     LVNNQERMTTASGQTLDYAISFRNHGSVVARNVLVADDLPAGLQYVPGSLRLNTRALT
                     DPADTDEGYSASLRRFEVRIAQVQPGEVVTLTFRALVAGDIAPGAGAVNTATLSGENF
                     DSVSTTAATAVVNPFGTVYAGRSGGAVTVNGARVSLVTDTTTRTPLPTTSGIGFAPNE
                     TNDNPYTTAQGGLFSFALTPAQRSAPATYYVNVTAPGYRSRMLEYSIRPAALHGLYHA
                     DVRALDEQPIAEQGSFALTDAPVRLDNLASVALNIPLFELQTLEINKIADQQRAEVGD
                     IVSYRIEVRNTTQGILRDVVVRDLLPQSFHYAAGTAQLTVSPAPPQNVEPQVKGNELT
                     FNVGTLPAGARASLVYRVRIGVNAQEGDQTNSATASGVYASGERIMTNASRAIVRVGR
                     GVFSTRQIIIGRVFEDKNGNDEFDAGERAVEGARLYLDNGQSVVTDSEGMYSLPSVED
                     GSVVIALDPVTLPRGYALTDEGRRSGRSWARLLRTPLGGGTLLRQNFALRSTNGASRS
                     DEEAKLDAQQPGGKQTPLRAPQLAAIETAPAPATSDAPASNATATNATTPDVQATHAL
                     APTTNASANVSGASPAREPRIEKISGAKETNGVAPDLSAGTYVVEAKEAVAAVAPGDV
                     LIVSPAQDEVVMSAAMQVEVRVAEAWTATLEVNGQHVGAQNIGLSRVDHKNKVATYTF
                     VGLNLRPGQNRVRAIAVSPAGQAGRAIEFNVMGRGPARRLEITTERNELQAGGRDSAL
                     LQVRAFDQWNNPAADGQVAVATSSGHFLRPAHEASNPAGVGEKERDKSLELTEEFAGR
                     NANATLREVILSLRGGVASVQLVSDNTAGVAAISAHTADIKAEHRLRFTAELRPAILV
                     GLAEASIGRAAPENTLRGDNSRARSHIEFFYRGSVFGKNLLTLAYDSQRSLNRTMGHD
                     RLFQADPLERAYPVFGDSSTRFNDVESNSKLYARIDRERSYAMFGDFEAGLSDTQLAS
                     YSRKLTGVKLHLENSNGDFVSVTGARPDTSFARDVFPASRLGLINLSYTDVLPGSETV
                     ALEVRDRRNPERIISREPLLRSLDYNLDPLTGQLFFMRPISAFDYDLNLVQLVVTYEH
                     RAAGMSSGVYTGRASKRFTRLGLRLGLSLVEQKQNEFGSYLLGGLDGEQTLPNRGSLK
                     FEWAMSKGDMATNGNIYNAAERHAGGNAYRLELTQPLDFYQALLHAEFARADEGFFNP
                     FGATVAPGSQRMGATLDLKPAASRIVRLGFLNERNHTANVDNSRNTFSLLWTETFTER
                     LHAFVGYDYRR"
     gene            complement(4665..6803)
                     /locus_tag="VGB76_08750"
     CDS             complement(4665..6803)
                     /locus_tag="VGB76_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099027.1"
                     /translation="MKISYTFPPALRRTLTRTLVALLATCSAFLTPALAQTSGGTTIS
                     NQASAVYTDSANNNYSTVSNTITVTVANVSGLRILPDDAVNPAVVPGDTNATFLFTVT
                     NTGNFADQVRFLASGQSLNLTGPGTITAAVIDMDSSNTITAGDINIFSNGADVLHALA
                     QNASATIIVRATISAAASAGDTVQVFLGDAATGAAQGYDNQTPDNPATPSVHEVRTVS
                     GTSVNGVREARGDIRVTVQNDVQLRAVLTVPAGPVALGGNINYSTQVCNDGNRTATSM
                     SLGAFSGIYIVMPVPAGTALSASNTFPAGTLYTTSALTTTPEAAVWTNTAPGTLSTVT
                     RVAFNAGATLASGGTCSASFTLIVAITTTDATTPIYALVDSFATNTIGTVLTDQSDSP
                     GGEVANRGDRNANFNEPRLSLDPVNPGQGIRQPTSLQQVGGVLIGPSGQPAATGPGNS
                     NNTDFTNRSVTTGIAGVPPCAGAPNAACSTNAAGFIVFANTVRNTGNATDTFTLTAPT
                     VPAGFTVGVSLNPAGPFTTISGGGSFVLAPLARNTNAEVYVRVDAPAVQAILTGFDSV
                     IQAASGNTPANTNLTIDRLYTGFVRLDKTVTVVDASDNPSATIPGAIMVYQITYTNIM
                     TAPPAGSNSSALTATSLVITEDGSAGGNNWATFTTQVTAPMPSISTAGGTVTDGAGNP
                     VTALTNVLKGNVPNLAPGETGTFRFRRLIN"
     gene            complement(6931..7461)
                     /locus_tag="VGB76_08755"
     CDS             complement(6931..7461)
                     /locus_tag="VGB76_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099028.1"
                     /translation="MKKLQKKMTAPTVAALCLLLVGTAAAAFAQRQIAARSSAGQPEV
                     KIKLSGSIKRDDQSLTLDKVSAVHPGEILDWTITSANEGNAPARKYKTIGQIPQGTSF
                     VAGSAVAEYGASVSYSIDGGKNFAAQPIIEERQADGTKKTVPAPASMYTQVRYDWNDP
                     LVAGSTLSASYKVRVK"
     gene            complement(7546..7938)
                     /locus_tag="VGB76_08760"
     CDS             complement(7546..7938)
                     /locus_tag="VGB76_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099029.1"
                     /translation="MAKVMICDDDRVLAEGLVAALRAAGHEAETCRHTMDVLRAAAAG
                     EFDLIAFGLDMAGFGRAGAVEVLREIAPRVPLIALHERPYEIMRAAASTRAQFAAVLP
                     RSLAVEDFMYAVARALSQPDTRRREPAA"
     gene            complement(8279..>9230)
                     /locus_tag="VGB76_08765"
     CDS             complement(8279..>9230)
                     /locus_tag="VGB76_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0099030.1"
                     /translation="MRGLVQIAEAAARLDSTVILTGESGTGKDVLARFIHSRSARGDS
                     PLITINCGALPESLFESEFFGHEKGSFTGATAMKRGLIEAADGSTLFLDEIGDLPAPM
                     QVKLLHFLEQGRFRRVGSTRDRASDVRIIAATNRRLAEDVRQGRFRTDLFYRLNVISL
                     HVPPLRDRREDIPALIDHFLTLYRERFQRPALTLSEGARRRLESFNWPGNVRELRNTI
                     ERAAALSESDEIEAAHFTQLTDHKENASPGASHTGDASSDPEAPTRTLDELERQHILR
                     VLEETGGNRERAAIILGISARTLYRKLREYEETKALTSDG"
BASE COUNT         1524 a         2600 c         3356 g         1750 t
ORIGIN      
        1 ggcgtcgata atcgtagccg acgaaggcgt gcaggcgttc ggtgaaagtc tccgtccaga
       61 gcagcgagaa ggtgttgcgc gagttgtcta cgttcgcggt gtggttgcgt tcgttcagga
      121 agccgaggcg gacgatgcgc gatgcggcgg gcttgagatc gagcgtcgcg cccatgcgct
      181 gcgagccggg agcgacggtt gcgccgaagg gattgaagaa gccttcgtcg gcgcgcgcaa
      241 actcggcgtg gagcagtgct tgataaaagt cgagcggctg cgtgagttcg aggcggtagg
      301 cgttgccgcc cgcgtggcgt tcggcggcgt tgtagatgtt gccgttcgtc gccatgtcgc
      361 ctttgctcat cgcccattcg aatttgagag agccgcgatt cggcagcgtc tgttcgccgt
      421 cgaggccgcc gagcaggtag gagccgaact cgttctgttt ctgttcgacg agcgacagac
      481 cgaggcgcag tccgaggcgt gtgaaacgct tcgaggcgcg ccccgtgtag acgccggaag
      541 acatcccggc agcgcggtgc tcgtaagtga cgacgagttg gacgagattg agatcgtagt
      601 cgaaggcgga gatggggcgc atgaagaaga gttgccccgt gagcggatcg agattgtagt
      661 cgagcgaacg caggagcggc tcgcgcgaga tgatgcgttc ggggttgcgg cggtcgcgca
      721 cttcgagggc gacggtttcc gaaccgggca acacgtccgt gtaggagaga ttgatgaggc
      781 cgaggcggct cgccggaaac acgtcgcgcg cgaaagaggt gtcggggcgc gcgcccgtca
      841 cgctcacaaa atcgccgttc gagttttcga gatgaagttt cacgcccgtc agtttacgcg
      901 aataactcgc aagctgcgtg tcggagagtc cggcttcgaa gtcgccgaac atggcgtagg
      961 agcgttcgcg gtcaatgcgc gcgtagagtt tcgagttcga ctcgacatcg ttgaagcgcg
     1021 tggaagagtc gccgaagacg gggtaggcgc gctccagagg gtcggcctga aagagacggt
     1081 cgtgtcccat cgtgcgattg agggaacgct ggctgtcgta agcgagcgtg agcaggttct
     1141 tgccgaagac cgagccgcga tagaagaact caatgtggct gcgcgcgcgc gaattgtcgc
     1201 cgcgcaaggt gttctccggc gcggcgcgtc cgatggaggc ttcggcgagg ccgacgagaa
     1261 tggccgggcg cagttcggcg gtgaagcgca ggcgatgctc ggctttaatg tccgccgtgt
     1321 gcgcgctgat ggcggcgacg cccgccgtgt tgtccgagac gagctgcaca gacgccacgc
     1381 cgccgcgcaa actcaagatc acttccctca aagtcgcgtt cgcattccgg cctgcgaact
     1441 cctccgtcaa ctccagagac ttgtcgcgct ctttctctcc cacgcccgcc gggttcgagg
     1501 cttcgtgggc ggggcgcagg aagtggccgc tcgaagtggc gacggcaacc tgcccgtcag
     1561 cggcggggtt gttccactgg tcgaaagcgc gcacctgcaa gagcgcggaa tcgcgcccgc
     1621 ccgcttgcag ttcgttgcgc tccgtcgtga tctcaagccg ccgcgccgga ccacgcccca
     1681 tcacgttgaa ttcgatggca cgccccgcct gtcccgcagg actcacggcg atggcgcgca
     1741 cgcggttctg accggggcgc aggtttagac cgacgaacgt gtaggtcgca actttgttct
     1801 tgtgatcgac gcggctcaat ccgatgttct gtgcgccgac gtgctggccg ttgacttcca
     1861 gcgtcgccgt ccatgcttcc gccacgcgaa cttccacctg catcgccgcc gacatcacca
     1921 cctcgtcctg cgcagggctg acgatgagca cgtcgcccgg cgcaaccgcc gcgaccgctt
     1981 ccttcgcttc gactacataa gtgcccgccg agagatcggg cgcaactccg tttgtctctt
     2041 tcgcaccgga gatcttttcg atacgcggtt cacgcgcggg cgacgcgccg gagacgtttg
     2101 cactcgcgtt cgtcgtcggc gcaagcgcgt gcgtcgcctg tacgtcaggt gttgtggcgt
     2161 tggttgccgt cgcgttcgag gcgggggcgt cggacgtggc gggcgcggga gccgtctcaa
     2221 tcgctgcgag ttgcggagcg cgaagcgggg tctgtttgcc gccgggttgc tgcgcgtcga
     2281 gtttcgcttc ttcgtcgctg cggctcgcgc cgttcgtgct gcggagcgcg aagttttggc
     2341 gcagcaaggt gccgccgccg agcggtgtgc gcagcaggcg cgcccaactg cgaccggaac
     2401 gacgcccttc gtccgtgagc gcgtagccgc gcgggagcgt caccggatcg agcgcgatga
     2461 cgaccgagcc gtcttcgacg gacggcagac tgtacatgcc ttccgaatcg gtgacgaccg
     2521 attggccgtt gtccagatag aggcgcgcgc cttcgaccgc gcgttcgccc gcgtcgaact
     2581 cgtcgttgcc gttcttatct tcgaagacgc gcccgatgat gatctgccgc gtggagaaga
     2641 cgccgcgccc gacgcgcacg atggcgcgcg acgcattggt catgatgcgc tcgcccgaag
     2701 catagacgcc cgacgccgtg gccgagttgg tttgatcgcc ttcctgcgcg ttgacgccga
     2761 tgcggacgcg atagacgagc gaagcgcgtg ccccggcggg gagtgtgccg acgttgaacg
     2821 tgagttcgtt gcctttgacc tgcggctcga cgttctgcgg cggcgcgggc gagacggtga
     2881 gttgcgccgt gcccgccgcg tagtggaacg attgcggcag gaggtcgcgg acgaccacgt
     2941 cgcgcagaat cccctgcgtc gtgttgcgca cttcgatgcg ataagaaacg atgtcgccga
     3001 cctccgcgcg ctgctggtct gcgatcttgt tgatctcaag cgtctgcaat tcgaagagcg
     3061 ggatgttgag agcgacgctg gcgagattgt cgagacggac gggcgcgtcc gtgagcgcga
     3121 agctgccctg ttcggcgatg ggctgctcgt cgagcgcgcg cacgtctgcg tgatagaggc
     3181 cgtgcagggc ggcggggcga atcgaatact ccagcatgcg cgagcgatag ccgggcgcag
     3241 tgacgttgac gtagtaagtg gcaggcgcgg agcgttgtgc cggagtgagt gcgaaactga
     3301 agaggccgcc ctgcgccgtc gtgtaggggt tgtcgttcgt ctcgttcgga gcgaagccga
     3361 tgccgctcgt ggtggggagc ggcgtgcgcg tcgtcgtgtc tgtgacgagc gagacgcgtg
     3421 cgccgttgac ggtgaccgcg ccgccgctgc gccccgcgta gactgtgccg aagggattga
     3481 cgacggcggt ggcggcggtg gtgctcacgc tgtcgaagtt ctcgccgctg agcgtggcgg
     3541 tgttgaccgc gcccgcgccc ggcgcgatgt cgcccgcgac gagcgcgcgg aaagtgagcg
     3601 tcaccacttc gccgggctgc acctgcgcga tgcgcacttc gaagcgacgg aggctcgcgg
     3661 aatagccttc gtcggtgtcg gcggggtcgg tgagcgcgcg cgtgttcagg cgcagagagc
     3721 cggggacata ttgaagcccg gcgggcagat cgtctgcgac gaggacgttg cgcgcgacga
     3781 ccgaaccgtg gttgcggaaa ctgatcgcgt agtcgagcgt ctggccggag gcggtggtca
     3841 tgcgctcctg attgttgacg agtttggagg gcgggagcgc ggggttcgtg gggttggtga
     3901 ggcgcgcgcg ttcgccgacg gcgttgatga tcgtgcccgt gtcttcgacg gtgccgttga
     3961 cggtcataga gacgttcgag cgcgcatcga ggcgtatgac gagttgcgtc tgcggcgcgc
     4021 tcgcgttcgt gtccacgacg gcgagcacgt tgatgcaggc gcgcggcgca aggcgcggcg
     4081 acatcgtgga gttgagattg atggaggtgt cggcgtcgct caaaacgccg ttgtcgtccg
     4141 tgtcgaagtg gagggagaag atggtggcgg gcgtggagac ttcggcgcgc gtgatggtgt
     4201 agaggtcggg cgtgttgccg aagttgcaga cgcggaagag gcgcgtgatg cgttcctgcg
     4261 gcgcgacggc ggcggaagga gcggcttcgt ccggcgtgac gacgagcgcg gagacggggc
     4321 ggacggtgac ggtgatggtg ggcgagacgg tggaaaactc gttgccttct tcgtccgcgt
     4381 agatcgcttc ggcgcggttg gagatcagag aatccgccgg agtctgttgc ggctgcgcga
     4441 agcccgcacc cggcgcgaaa gcgagcacga gcaacagggc ggcgatcaca aacttgtcgg
     4501 cggcggtctt caacattggt gaatgaggtt cagcggcagt tcattcaaaa acgcgtggtt
     4561 ggcgtaaata aaaatggaag cggcgcagct tgccggaacg tgaacggggc acagactcac
     4621 gttccaccgc gccgcttcca aaccgggcgc gggttaaatc agccctagtt gatcaagcga
     4681 cggaagcgga acgtgcccgt ctcgccgggc gcgagattcg gcacgttgcc cttgagcacg
     4741 ttcgtcaagg ccgtgacggg attacccgcc ccgtctgtca cagtgccgcc ggcggtcgag
     4801 atgctcggca tcggcgcagt gacctgcgtg gtgaacgtcg cccagttgtt accgcccgcg
     4861 ctgccgtctt ccgtgatgac gaggctcgtc gccgtcaacg cggaactgtt cgaaccggcg
     4921 ggaggcgcgg tcatgatgtt tgtgtaagtg atctggtaca ccatgatcgc gccggggatg
     4981 gtggccgagg gattgtccga agcatcgacc acggtgaccg tcttgtcgag ccggacgaat
     5041 cccgtgtaga gacggtcaat cgtcaggttc gtgttggcgg gcgtgttgcc ggaagcggcc
     5101 tgaataacgc tgtcgaagcc ggtcaggatc gcttgcacgg cgggcgcatc cacgcgcacg
     5161 tagacctctg cgttggtgtt tcgggcgagc ggggcgagca cgaaactgcc gccgccggag
     5221 atggtcgtga aaggaccggc ggggttgagg ctgacgccga cggtgaagcc agcgggcacg
     5281 gtcggcgcgg tgagcgtgaa cgtgtctgtg gcgttgcccg tgttgcgcac ggtgttggcg
     5341 aagacgatga aacctgccgc gtttgtcgag caggccgcgt tcggcgcgcc cgcgcagggc
     5401 ggaacaccgg cgatgcccgt ggtcacgctg cggttggtga agtctgtgtt gttgctgttg
     5461 cccggtccgg tggcggcggg ttggccgctc ggcccgatga gaacgccgcc cacctgttgg
     5521 agcgaggtcg gttgccggat accctgaccg ggattgacgg ggtcgaggga caggcgcggc
     5581 tcgttgaagt tggcgttgcg gtcgccgcga ttcgctactt cgccgcccgg cgagtccgat
     5641 tgatcggtca agaccgtgcc gatggtgttg gtggcgaatg agtccacgag ggcatagatc
     5701 ggcgtggtgg cgtcggtcgt cgtgatggcg acgatgagcg tgaaactcgc gctgcacgtg
     5761 ccaccgctcg cgagcgtggc gcccgcattg aaggcgacgc gcgtcacggt ggagagcgtg
     5821 ccgggcgcag tgttggtcca cacagccgct tcgggggtgg tggtgagcgc gctcgtcgtg
     5881 tagagcgtcc cggcggggaa ggtgttgctg gccgagagcg cggtgccggc gggaaccggc
     5941 atcacgatgt agatgccgct gaatgcgccg aggctcatcg aggtggccgt gcggttgccg
     6001 tcgttgcaaa cctgcgtact gtagttgatg ttgccgccga gagcgacggg gcctgccggg
     6061 acggttaaga cggcgcgcag ttgcacgtcg ttctgcacgg tgacgcggat gtcgccgcgc
     6121 gcttcgcgga cgccgttgac cgaggtgccg gagacggtgc ggacttcgtg caccgagggc
     6181 gtcgccggat tgtcgggcgt ctgattgtca taaccttgag ccgcgccggt cgccgcgtcg
     6241 ccgaggaaga cctgcacggt gtcgcctgcg gaggctgccg cgctgatcgt cgcgcgcacg
     6301 atgatggtgg cggaagcgtt ctgcgcgagc gcgtggagca cgtctgcgcc gttcgagaag
     6361 atgttgatgt cgcctgcggt gatcgtgttg ctgctgtcca tgtcgatgac ggcggcggtg
     6421 atcgtgccgg gtccggtcag gttcaaactc tgaccgctgg cgaggaagcg cacttgatcg
     6481 gcgaagttgc ccgtgttggt cacggtgaag aggaaggtgg cgttagtgtc gccggggacg
     6541 accgccgggt tgacagcatc gtcgggcagg atgcgcaggc cggagacgtt tgccaccgtc
     6601 acggtgatgg tgttggagac ggtgctgtag ttgttgtttg cgctatcggt atagacggcg
     6661 ctggcctggt ttgagatggt tgtgccgccg ctggtttgcg caagcgcggg ggtgagaaac
     6721 gcggaacagg tcgccagcag ggcgacgagc gtgcgcgtca aggtgcggcg taaggccgga
     6781 gggaaggtgt aacttatctt catatcaatc ctcaagtttg tgatagggag actcgccggt
     6841 tacgggaggt cgcttgtgat gtccgaagga gtttgaagag acataccaga gcgaggtgcc
     6901 tctcccgagc agcgtgcgac gagagcgcgc ttatttgacg cgcactttgt aggaagcgga
     6961 gagcgtcgag ccagccacga gcgggtcgtt ccagtcgtag cgcacctgcg tgtacatcga
     7021 agcgggggcg ggcacggtct tcttcgtgcc gtcggcctgt cgctcttcga tgatgggctg
     7081 cgccgcgaaa ttcttgccgc cgtcaatgct gtagctgacc gacgcgccgt actccgcgac
     7141 cgcgcttccg gcgacgaaag aagtgccctg cggaatctgg ccgatggtct tgtacttgcg
     7201 cgcgggcgca ttgccttcgt tcgcgctcgt gatcgtccag tccaaaatct caccgggatg
     7261 gacggcggaa actttatcga gcgtgaggct ctgatcgtcg cgcttaattg agccggagag
     7321 cttgatcttc acttccggtt gaccggcgga cgagcgcgcg gcgatctgtc tctgtgcgaa
     7381 cgcggcggca gctgtgccga cgagcaacag gcagagcgcg gccacggtcg gcgcggtcat
     7441 ttttttctgt aacttcttca tgaggtatct ccttcaatta gtctgaaaat tcttcggacg
     7501 aggttggcta tctgttggtt aatgtctgtt ccgagagttg ctgtttcagg cggcaggttc
     7561 tctccggcgg gtgtctggtt ggctgagcgc gcgggcgacg gcgtacataa aatcttcgac
     7621 ggcgagcgag cgcggaagca cggcggcaaa ctgtgcgcgg gtcgaagccg ccgcgcgcat
     7681 gatctcgtag gggcgttcgt gcagcgcgat gagggggacg cgcggcgcga tctcgcgcag
     7741 cacttccacc gcgcccgcgc gcccgaatcc ggccatgtcg agtccgaagg cgatgagatc
     7801 gaattcaccc gccgccgccg cccggagcac gtccatcgtg tgacggcacg tttcggcctc
     7861 atgcccggcg gcgcgcaaag cggccaccag cccttcggcc agcacgcggt cgtcatcaca
     7921 gatcattacc ttcgccatcc gcaattctcg tttcgctcaa actgttcttc tgaccaacaa
     7981 aacgcagttg gtcggacaca cacaagtcaa ttcacatgcc gcgcccggag acggcgcgag
     8041 aacgcaggct aaataaggaa atatgaaggg ttagcggaaa cgctgaaggc tggatggagt
     8101 cggcggacgg acacgcatga caaacgcgcc tgacgttttg tcagccgcag gacaaaatgg
     8161 cagaaggtga cgagtgacga gtgacgagag aagaaagtga attatcaaag gtgaataggg
     8221 acagcagttt taattctatt cacctttgac gattcacttt cttcccgctc gccactcgtc
     8281 agccgtcgct cgtcaatgct ttcgtctctt cgtattcgcg cagcttgcga tagagggtgc
     8341 gtgcggagat gccgaggatg atggcggcgc gttcgcggtt gccgcccgtc tcttcgagga
     8401 cgcgtaggat gtgctggcgt tcgagttcgt cgagcgtgcg ggtgggagcc tccgggtcag
     8461 aggaagcgtc gccggtgtgc gatgcgccgg gcgaagcgtt ctctttatgg tcggtgagtt
     8521 gtgtgaagtg cgcggcttcg atctcgtcgg actcggagag cgcggcggcg cgttcgatgg
     8581 tgttcctgag ttcgcgcacg ttgcccggcc agttgaaaga ttcgaggcgg cgacgcgccc
     8641 cctccgagag cgtgagcgcg ggtctttgaa aacgctcgcg gtagagcgtg aggaaatggt
     8701 cgatgagcgc ggggatgtct tcgcggcggt cgcgcaaggg gggcacatgc agcgagatga
     8761 cgttcagacg atagaagagg tctgtgcgaa agcgaccctg ccgcacgtct tctgcgagac
     8821 gacgattggt ggcggcgatg atgcgcacat cgctcgcgcg gtcgcgcgtc gagccgacgc
     8881 ggcggaagcg tccctgttca aggaagtgca gcagtttgac ttgcatgggc gcgggcaggt
     8941 cgccgatctc gtcgaggaag agcgtcgagc cgtcagccgc ttcgatgagt ccgcgcttca
     9001 tcgcggtcgc gcccgtaaac gatcccttct cgtggccgaa gaactccgac tcgaagagcg
     9061 attcgggcaa cgcgccgcag ttgatcgtga tgagcggaga gtcgccgcgt gcgctgcggg
     9121 aatgaatgaa acgcgcgagc acatctttgc ccgtgccgga ttcgcccgtg aggatgacgg
     9181 tcgaatcgag acgcgccgcc gcttcggcga tctgcaccag cccgcgcatc
//
LOCUS       DASWNS010000247         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_372843, whole genome shotgun sequence.
ACCESSION   DASWNS010000247 DASWNS010000000
VERSION     DASWNS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372843"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VGB76_08770"
     CDS             <1..309
                     /locus_tag="VGB76_08770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0099031.1"
                     /translation="RGGESASGGRFPDEINRSSDLYKGAYTKGTVAMANAGPNTNGSQ
                     FFIMHADYPLPPSYSKFGRVIEGQEVVDAIASVAKDSSDRPRTDVVMEKVTIEAGDGQ
                     "
     gene            complement(427..1317)
                     /gene="thiD"
                     /locus_tag="VGB76_08775"
     CDS             complement(427..1317)
                     /gene="thiD"
                     /locus_tag="VGB76_08775"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY0099032.1"
                     /translation="MSGLHTNGKEHSLVPINDPAEMPVALTIAGFDPSGGAGVIADVK
                     TFTAFGCFATAAITSLTFQNTTGVFGATHVTAADVRAQVLPVVEDFHVAGVKTGMLPT
                     REVISEVARLLRETHLRESYAIVDPVVRSTSGFDLIDDDALAALRSELLPLARLSTPN
                     IPEAERLTGLRIETEEEMRRAARMLREMGARAVLVKGGHLDAREAVDLLDDAGEVRLF
                     RAPRIQTDATHGTGCTLAAAIAACLARGMSLPDAVGAAKRFVTEAIRRSPQLGRGFSP
                     VNHNLRVRFDERDGVLIAED"
     gene            complement(1292..1576)
                     /locus_tag="VGB76_08780"
     CDS             complement(1292..1576)
                     /locus_tag="VGB76_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099033.1"
                     /translation="MPTIKSIEGKIKRVERFAVKILYPDGSDIRSDQASFKHTYNYEL
                     AAKDDMTVSHWKASRFQKIFQGFKVEVLLGNGQTANGNTKLSNVRASYER"
     gene            complement(1677..2336)
                     /locus_tag="VGB76_08785"
     CDS             complement(1677..2336)
                     /locus_tag="VGB76_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0099034.1"
                     /translation="MPLDPTTPISYLVTDGRTTEATNSSSAEFTRLFVLVGACVEAGV
                     SLVQLREKSMRPRVLYELTRRAAELTRGTSTRLLVNDRADIALAARADGVQLTTRSLA
                     PLIVRDISPPGFLIGVSTHALAEAQAASASDADFALFGPVFDTPEKRAYGAPLGLEPL
                     REAARALAPFPLLAIGGITRESIPQVLQAGARGIAAISLFQDESELEAIVNEISGSGG
                     L"
     gene            complement(2308..>2547)
                     /locus_tag="VGB76_08790"
     CDS             complement(2308..>2547)
                     /locus_tag="VGB76_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099035.1"
                     /translation="KYFLVYVAQKTGLHGLQQAIASGWVRGAVTGLGLVNFALAFWEI
                     ANFRRTVSALNGQAPDQNAPAAPLSPHAARPDHAD"
BASE COUNT          462 a          746 c          867 g          472 t
ORIGIN      
        1 cgcggcggcg agtctgcttc gggcgggcgt ttcccggacg agatcaaccg ttcgtccgac
       61 ctctacaagg gcgcgtacac gaaaggcact gtggcgatgg cgaacgcggg gccgaacaca
      121 aacggctcgc agttcttcat catgcatgcc gactatcctc tgccgccgag ctacagcaag
      181 ttcgggcgcg tcatcgaagg gcaggaagtt gttgacgcca tcgcgtcggt ggcgaaagac
      241 agcagcgacc gcccgcgcac ggatgtcgtg atggaaaaag tgaccatcga agcaggcgac
      301 gggcaataag cgaccgttga agagaaacga tgaaggcgga aggatgaagg atgaaggaga
      361 agcaactggc ttctattcat ccttcatcct tccgccttcg tcattacatc aattttctcg
      421 cctgctttaa tcctcagcga tcaacacgcc gtcacgttca tcgaagcgca cgcgcaagtt
      481 gtgattgacc gggctgaagc cgcgccctag ttgcggcgag cggcggatgg cctcggtgac
      541 gaaacgcttg gccgcgccga ccgcatccgg caggctcatc ccgcgcgcaa gacaggctgc
      601 gatagcggcg gcgagcgtgc agcccgtgcc gtgcgtggcg tccgtctgga tgcgcggtgc
      661 gcggaagagt cgcacttcgc ccgcgtcgtc gagcaggtca accgcctcgc gcgcgtcgag
      721 atgcccgccc ttaacgagca cggcgcgtgc gcccatctcg cgcaacatgc gtgcggcgcg
      781 gcgcatctcc tcttccgtct cgatgcgcag gccggtgagg cgttcggctt cggggatgtt
      841 gggcgtcgag aggcgcgcga gcggcagcag ttccgaacgc agggcggcga gcgcgtcgtc
      901 gtctatgagg tcgaagccgg aggttgagcg cacgaccgga tcgacgatgg cgtacgactc
      961 gcgcaggtgc gtctcacgca aaagccgcgc gacctccgag atcacttcgc gcgtcgggag
     1021 catccccgtc ttgacgcccg cgacatggaa atcttcgacg acgggcaaga cctgtgcgcg
     1081 cacgtccgcc gccgtcacgt gcgtcgcgcc gaacacgccc gtcgtgttct ggaaggtgag
     1141 cgacgtgatg gcggcggtcg cgaagcagcc gaaggccgtg aaggtcttaa cgtcggcgat
     1201 aacgcctgcg ccgcccgaag gatcgaagcc cgcgatggtt agggcgacgg gcatctcagc
     1261 cggatcattg atgggcacga gagaatgttc cttaccgttc gtatgaagcc ctgacattgg
     1321 agagttttgt gttgccgttg gcggtctgcc cgttgccgag cagcacttca actttgaaac
     1381 cctgaaatat tttctggaag cggctggcct tccaatggct gaccgtcatg tcgtctttcg
     1441 ccgccaactc gtaattataa gtatgtttga aacttgcctg atcactgcga atgtcgctcc
     1501 cgtccgggta gagaatcttc accgcaaacc tttccacgcg cttaattttt ccttcgatac
     1561 ttttgatagt tggcatcttc acctccggtc gaaagtgtgg ctgcgttcca ttatggattg
     1621 aaaagcgtga agaggtcacg cggcctcatc ttcgatctcg cgtccgcgcc tgaaatttag
     1681 aggccgccac ttccgctgat ctcgttgacg atggcttcga gttcgctttc gtcctgaaag
     1741 agactgatgg cggcaatgcc tcgcgcgcct gcctgtaaaa cctgcggaat gctttcgcgt
     1801 gtgatgccgc cgatggcgag aagcgggaag ggcgcaagcg cgcgtgccgc ttcgcgcagg
     1861 ggttcgaggc cgagcggcgc gccgtaagcg cgcttttccg gcgtgtcgaa gacggggccg
     1921 aagagggcga agtctgcgtc gctcgcgctg gccgcctgcg cttccgcgag cgcgtgtgtg
     1981 gagacgccga tgaggaaacc gggcggggag atgtcgcgga cgattagggg agcgagcgag
     2041 cgcgtggtga gttgcacgcc gtcggcgcgg gcggcgagcg cgatgtcggc gcggtcgttg
     2101 acgaggaggc gcgtcgaggt gccgcgcgtc aattcggcgg cgcggcgcgt gagttcgtag
     2161 agcacgcgcg ggcgcatgga tttctcccgc agttggacga gcgagacgcc cgcttcgacg
     2221 catgcgccga cgagcacgaa caggcgcgtg aactccgcgc tcgaagagtt ggtggcttca
     2281 gtcgtgcgcc cgtcggtgac gagataacta atcggcgtgg tcgggtcgag cggcatgggg
     2341 cgaaagcggc gcggcgggcg cgttctggtc gggggcttgg ccgttgaggg cgctcacggt
     2401 gcggcggaag ttggcgatct cccagaaggc cagcgcgaag ttgacgaggc cgagtcccgt
     2461 gaccgcgccg cgcacccaac cggaagcgat ggcctgttgc aaaccgtgca agcccgtctt
     2521 ttgcgccacg tagacgagga agtattt
//
LOCUS       DASWNS010000248         5946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_243779, whole genome shotgun sequence.
ACCESSION   DASWNS010000248 DASWNS010000000
VERSION     DASWNS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243779"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..988
                     /locus_tag="VGB76_08795"
     CDS             341..988
                     /locus_tag="VGB76_08795"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY0099036.1"
                     /translation="MPIYDISVAISPETTPTYPGDPAIEIVSWAAIARGDAANVTFLH
                     MGAHTATHVDAPAHFIEHAPQVSAMPLDALIGEARVVELDETIECINASHVAAHVPAG
                     TTRVLFKTRNSNFWRSEGRAFRPDFTHLTLEGARALKERGVRLVGIDYLSIEKFKSET
                     FETHTTLLSAGVVIVEGLDLHEVTAGLYELICLPLKIAAGSGDGAPARAVLRTPD"
     gene            1023..1382
                     /locus_tag="VGB76_08800"
     CDS             1023..1382
                     /locus_tag="VGB76_08800"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0099037.1"
                     /translation="MEVVNLNDKFSLFQEHWKPKIAGEVNDAYIKLVKFKGEFLWHKH
                     EAEDELFLVVKGNLTIKLRDRDLQLAEGEFAVIPRGVEHMPVAEEEVQVMLIEPKTTL
                     NTGDRQTNRTLPELERI"
     gene            1392..1547
                     /locus_tag="VGB76_08805"
     CDS             1392..1547
                     /locus_tag="VGB76_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099038.1"
                     /translation="MNSPSEKRKIFASERARFARLSVPRLIQLLSSPDLRTRFLAEMC
                     LRDATNT"
     gene            1664..2116
                     /locus_tag="VGB76_08810"
     CDS             1664..2116
                     /locus_tag="VGB76_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY0099039.1"
                     /translation="MVTKSKRSDDGQDLKAAKASQGVPAKAGEPGYNARVFEIVRRIP
                     AGHVMTYGQLAEILGENYTARTVGFAMHAADDSVPWHRVINAQGACSTGRVLLPLNKQ
                     QRMLEAEGIEFDARDRCDLGRYRWTPEEARVNDDSNDDEGSQPSLFGD"
     gene            2155..2568
                     /locus_tag="VGB76_08815"
     CDS             2155..2568
                     /locus_tag="VGB76_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEY0099040.1"
                     /translation="MNNDPLQTRAPYEISERRYSVSPHMVTTAFELPGFRVTRNLGIV
                     RGIVVRSRNLFVNIGAVFNSMVGGNITAWTNLCEQTRREAFEIMVQHATELGANAIIG
                     ARYDATELGSGITEVLAYGTAVIVEATDTTELYNS"
     gene            2565..2987
                     /locus_tag="VGB76_08820"
     CDS             2565..2987
                     /locus_tag="VGB76_08820"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0099041.1"
                     /translation="MNETPLTEAELGRLREAITRVPYAQLLGIKFEDATHGTASLSMC
                     ARAELERFGGIMHGGALASLADSASAFAVLSTLEPEEQTVTVDLTLHYLRPVTEGKLT
                     AHARVLRAGRRVATVSVEVFNESGTLVMTALTTYLKLS"
     gene            3201..4889
                     /locus_tag="VGB76_08825"
     CDS             3201..4889
                     /locus_tag="VGB76_08825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY0099042.1"
                     /translation="MLSLYQAIVSQHSRQLTPVRCADQTIAQLHRYFEDVVLENNLAA
                     LVVESLPLQVERSPRSAEHVLKVVRAARYAFFFLSPEDAYSQMPLLDEARESMLLSRA
                     AQGGNNEHFVIIADARFSALLVSVSGQTNDGAETTTESEVIWTFEPDIVYSALEYLMA
                     RVTAERFEQTEAFAQAVRTCMPKATSLQLTVSVTTKLARLLQEQAAREVAINRIASAI
                     RSSLSLPSVLQTTVDEVGRALGVQLSGLHVEGENEQAPMMMKCYFRDGAPSEEERETL
                     VNDLYAYHTQLARRLKHYVLDEQRGSSAQTEDETRPLAVVPLLYQARSMGVLLVSSDD
                     PHRIWQENELLLLRTVADQVAVAVNHARLFTQMQQLALTDGLTGCVNRRSFEMQLERD
                     LRLATRMRQPVSLIMLDIDHFKRVNDTYGHDAGDAALRFLADVLRDELRGVDTAARYG
                     GEEFAVILPQAGLEGALIVAERLRSRLEMTEVPGIGHITASFGLATFPLHANSRDQLV
                     GSADCALYESKHAGRNRICTPPSAQTEVSDEMYGAPIEIAAQAEADSIIQTPAS"
     gene            5165..>5946
                     /locus_tag="VGB76_08830"
     CDS             5165..>5946
                     /locus_tag="VGB76_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY0099043.1"
                     /translation="MDDLKKFTLYFRPYKKELITGIICIVASVVVGLFIPLLVGRAVD
                     GLRADVTWAKLTRYALVILGVSLISGTFLFLQRRILIGMSRHVEFDLRQDFYRHLQQL
                     PLSFFQSNRTGDLMARATNDLAAVRQLAGPMIMYTLQTVLVVVLILPLMLRISVRLTL
                     LLFVTMPLVSLTVKFFGQQVHTRFEKIQDFFAQISARAQENFTGVRVVRAYAQEEAEI
                     AVFKNLNREYAERNLGLVRIDAVMRPLMQFLIGLGFVLILWYG"
BASE COUNT         1242 a         1842 c         1679 g         1183 t
ORIGIN      
        1 gctcgccccg cgcaaaactc cagatgagcg gagccgagtt cagataactc gaagccgcca
       61 cacgcggcac gccctgatgt ccgacgctct ctgaaatgac tctcgctcct ctcaacccga
      121 aatccccgac cgcgcccgcc aactctttca atcagatgct aacgcacggc agagaggctc
      181 gcaagaagtc atgcgttatt aaaccaacgc cgcgttgaca gccctcgaaa gctcccttta
      241 acatgacgaa gtaatgagtg atgagtaatg agtgatgagt aaaaacagaa gtcttgtctt
      301 ttactcatca ctcattactc atcactcatt actcaaacaa atgccaattt atgacatcag
      361 cgtcgccatc tcgccggaga cgacgccgac ttatccgggc gatcccgcca tcgagatcgt
      421 ttcgtgggca gctattgcgc gcggcgatgc cgccaacgtc actttcctgc acatgggcgc
      481 gcatacggcc acccacgtcg atgctcccgc tcacttcatc gaacacgcgc cgcaggtttc
      541 tgccatgccg ctcgacgcgc tcatcggcga agcgcgcgtc gttgaattag acgagacaat
      601 cgagtgcata aatgcgagcc acgttgccgc gcacgtcccc gcaggcacga cgcgcgtgct
      661 cttcaaaacg cgcaactcca atttctggcg cagcgaaggg cgtgcgtttc gcccggactt
      721 cacacacctt acgctcgaag gggcgcgtgc gctcaaggaa cgcggcgtgc ggctcgtcgg
      781 catcgattat ctgtccatcg aaaagttcaa gtcggaaact ttcgagacac acacgacgtt
      841 gctctcggca ggagtcgtta ttgtcgaagg actggacttg catgaggtga ccgccgggct
      901 ctacgaactg atctgcctgc ctttaaagat cgcggcgggc agcggcgacg gtgcccccgc
      961 acgcgccgtc ctgcgcacgc ccgactagct ggcctcgctt tcaaaccgtc aggagcaacg
     1021 caatggaagt agttaatctc aacgacaagt tctctctctt tcaagagcac tggaagccga
     1081 agatcgcggg cgaggtcaac gatgcttaca tcaagctcgt taagttcaaa ggcgaatttc
     1141 tctggcacaa gcacgaagcg gaagacgaac tctttctcgt cgtcaaagga aacctgacga
     1201 tcaaattaag ggatcgtgat ctgcaactgg cggagggcga gtttgcagtt atccccagag
     1261 gcgtcgagca catgccggtg gccgaagagg aagtgcaagt catgctcatc gagccgaaga
     1321 ccacgctcaa cacgggcgac cggcaaacaa accgcaccct gcccgaactc gaacgcatct
     1381 gaaccgccgg gatgaattca ccaagcgaga aacgaaaaat cttcgcctcc gaacgcgcac
     1441 ggttcgcgcg gctctccgtc ccgcgtctga tccaactgct gtcgagtccg gatctgcgca
     1501 cgcgctttct ggccgagatg tgcctgcgcg atgcgaccaa cacctgactc agtttccagc
     1561 ctgcggctcc gcaggccgaa cgcacgcgcg cgattgcaga gtggcgcgca tggtggcgcg
     1621 agcataaccc ggcggttaag agacgccgtt cacaagatca aagatggtca caaaatcaaa
     1681 acgaagcgac gacggacaag acttaaaggc ggcgaaggcg tcgcagggcg ttccggccaa
     1741 agcgggcgaa ccgggttaca acgcacgcgt cttcgagatc gtgcgccgca tccctgccgg
     1801 gcacgttatg acctacgggc agcttgcgga aattctcggc gagaactaca cggcgcgcac
     1861 cgtcggcttc gccatgcacg cggcggacga ctccgtgccc tggcatcggg tcatcaacgc
     1921 gcagggcgca tgctccacgg ggcgcgtgct gctgcccttg aacaagcagc agcgcatgct
     1981 cgaagcggaa ggcatcgagt ttgacgcgcg cgaccgttgc gatctcggtc gttatcgctg
     2041 gacgccggag gaggcgcgtg tgaacgacga ctcgaacgac gatgaaggct cgcagccgtc
     2101 gctcttcggc gattgacagt tttccattaa ccgctcgcgg acagctatca aaatatgaat
     2161 aacgatcccc tccagacacg cgcaccttac gagatcagcg aacgccgcta ctccgtctcg
     2221 ccgcacatgg tgacgaccgc ctttgagctt cccggctttc gcgtgacgcg aaatctgggc
     2281 atcgtgcgcg gcatcgtcgt gcgctcacgc aaccttttcg tcaacatcgg cgcggtcttc
     2341 aattcgatgg ttggcggcaa catcaccgcg tggacgaacc tctgcgaaca aacccgccgc
     2401 gaggcgttcg agatcatggt gcaacacgcc accgaactcg gcgcaaacgc catcatcggc
     2461 gcgcgttacg atgcgaccga gttggggagc ggcatcacgg aggtgctcgc ctacgggacg
     2521 gcggttatcg tggaagcgac ggacaccacg gaactctaca actcttgaac gaaactccac
     2581 tcacagaggc agagttgggt cgcctgcgcg aagcaatcac acgcgttccc tacgcgcaac
     2641 tgctcggcat taaatttgaa gacgcgacgc acgggacggc ctctctgtcc atgtgcgccc
     2701 gcgcggagtt ggaacggttc ggcggcatca tgcacggcgg cgcgctcgct tcgctcgccg
     2761 actcggcctc ggctttcgcc gtcctctcca cgctcgaacc cgaagagcag accgtcaccg
     2821 tagacctcac cctgcactac ctgcgtcccg tcaccgaagg caaattaacc gcgcacgcac
     2881 gcgtcctgcg cgccggtcgc cgcgttgcca ccgtctccgt cgaggttttc aacgagtccg
     2941 gcacactcgt catgacggct ttgacgactt atctgaaact aagttaagtc gttcagtttg
     3001 tgtaactaag gtcgccagag tgaagaaaga ggggcggggt ttcggttatt tataaatgct
     3061 ctgtaaatat tcatagacaa aacctgttaa ttaatgcaaa atatgttttg tcttcgtcgc
     3121 ttctgtagcg gcggcgaaag ttgctatccg cagttcctct tgagcgagcc tcatctggtc
     3181 agtctggtca cataagtctc atgctttctc tttatcaagc gatagtttcc caacactccc
     3241 gccaattgac tccggtgcgc tgcgcagacc agacgattgc gcaacttcat cgctatttcg
     3301 aagacgtggt gttggagaac aacttggccg ccctcgtggt ggagagtttg cctttgcagg
     3361 ttgagcgttc gccgcgttcg gcagagcacg tgttgaaagt tgtccgcgcc gctcgctacg
     3421 ccttcttttt cctctcgccc gaagacgctt acagccagat gccgctgctg gatgaggcgc
     3481 gcgagtccat gctgttgtcg cgcgcggctc agggcgggaa caacgaacat ttcgtgatca
     3541 tcgccgatgc gcgtttctcc gccctactcg tctccgtgag cgggcagaca aacgacggcg
     3601 cggaaaccac cacggagagc gaggttatct ggacgtttga gccggacatc gtctattccg
     3661 cgctggaata tttgatggcg cgcgtgacgg ctgaacgctt cgaacagacg gaagccttcg
     3721 cgcaagccgt gcgcacctgc atgccgaagg ccacttcgct gcaactcacc gtctcggtca
     3781 ccaccaaact cgcacgcctc ctgcaagagc aggccgcacg cgaagtcgct atcaaccgca
     3841 tcgcgtcggc gatccggagt tcgctctcgc tgccgagcgt gttgcagacg accgttgacg
     3901 aagttggccg cgcgctcggc gtacaactga gcggtctgca cgtcgaaggc gaaaacgagc
     3961 aggcaccgat gatgatgaag tgctacttcc gcgacggcgc gccgagcgaa gaggaacgag
     4021 aaacgctcgt caacgatctc tacgcttacc atacgcaact cgcacgtcgc ctcaagcact
     4081 acgtcctcga cgagcagcgc ggttcgagcg cgcagaccga agacgagacg cgcccgctgg
     4141 ccgtcgtgcc gctgctctat caagcgcgct cgatgggcgt gctgctggtt agttccgacg
     4201 atccgcatcg catctggcaa gagaacgaat tgctgctcct gcgcaccgtc gccgaccagg
     4261 tggcagtcgc cgtcaatcac gcgcgactgt ttacgcagat gcagcagttg gctctgacgg
     4321 acgggttgac gggatgtgtc aatcgtcgtt ccttcgagat gcagcttgag cgcgacctgc
     4381 gactggcgac gcgcatgcgt cagcccgtct ctctgatcat gctcgacata gaccacttca
     4441 agcgcgtcaa cgacacctac gggcacgacg cgggcgatgc cgcgctgcgc ttcctcgcgg
     4501 acgtgttgcg cgacgagttg cgcggcgtgg acactgcggc gcgttacggc ggcgaagagt
     4561 ttgccgtcat ccttccgcag gccggactcg aaggagcgtt gatcgtggcc gagcgtttgc
     4621 gctcgcgtct ggagatgacc gaagtgcccg gcatcggcca catcacggct tctttcggcc
     4681 tcgctacgtt tccgctgcac gccaactcgc gcgatcaact cgtcggctca gccgactgcg
     4741 cgctctacga atccaaacac gcgggacgca atcgcatctg caccccgccg agcgcgcaga
     4801 ccgaagtgag cgacgaaatg tacggcgcgc ccatcgagat cgcggcacag gccgaagcgg
     4861 attcgataat tcaaacgccc gccagctgag acgtagagca aaggaaaaag gggaaggggt
     4921 aaaggagaag tcgaatcttc ctttgcccct tctccttttc ctttcacctt ttacttcttg
     4981 cacgtctctt ccccggaata ctttcttcat ccacttgcac tacaattaag gagacttgcg
     5041 ccgcaccgga cagacgtttg ggtcgaggtc tggctttgga gagaaaaatc gcgcatcata
     5101 ggcgcgacga actcgttgcc ggagcggatc aatgttgcgt ggcgacgcgc cagtctcctt
     5161 tgttatggac gatctaaaaa aattcactct ctattttcgc ccgtataaaa aagaactcat
     5221 cacgggaatc atctgcatcg tggcctctgt ggtagtcggg ctgtttatcc ccttgctcgt
     5281 cgggcgcgcc gtggacgggc tgcgcgcaga cgtgacgtgg gcgaaattga cgcgctacgc
     5341 gctcgtcatt ctcggcgtca gtctgatcag cggcacgttt ctctttttgc agcgacgcat
     5401 cctcatcggc atgtcgcgcc acgtcgagtt cgatctgcgg caggactttt atagacacct
     5461 gcaacaactc ccgctctcct tctttcagtc caaccgcacg ggcgatttga tggcgcgtgc
     5521 gacgaacgat ctggccgccg tgcgccaact cgccgggccg atgatcatgt acacgctgca
     5581 aaccgtcctc gtcgtcgtgc tgatcctgcc cttgatgctg cgcatcagcg tgcgcctgac
     5641 gctgctgctg ttcgtgacga tgccgctcgt ctctctgacg gtgaaattct tcgggcaaca
     5701 ggtgcacacg cgtttcgaga agatacagga tttcttcgcg cagatttccg cccgcgcgca
     5761 ggagaatttt acgggcgtgc gcgtcgtgcg cgcctacgcg caggaggaag cggagatcgc
     5821 ggtcttcaaa aatctcaacc gcgaatacgc cgaacgcaat ctcggactcg ttcgcatcga
     5881 cgccgtgatg cgaccgctga tgcaattcct gatcgggttg ggcttcgtcc tgatcctctg
     5941 gtacgg
//
LOCUS       DASWNS010000249         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_243799, whole genome shotgun sequence.
ACCESSION   DASWNS010000249 DASWNS010000000
VERSION     DASWNS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243799"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..335)
                     /locus_tag="VGB76_08835"
     CDS             complement(138..335)
                     /locus_tag="VGB76_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099044.1"
                     /translation="MADEKRTDNDAVMNTITDETGQFDARFLLWRTFCAENDVPVETL
                     PSDLTGDIRDKWNKLKDSKLK"
     gene            complement(466..1377)
                     /locus_tag="VGB76_08840"
     CDS             complement(466..1377)
                     /locus_tag="VGB76_08840"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY0099045.1"
                     /translation="MKHEDYKELIALAALDALDDEGERHALAEHLAECADCRAAWDAE
                     RAAAASLIYATSPVAPAPELRAQILSRIKSQPQDEREEAARLPASSGNGAPNATTTAA
                     TSTAAVASNVSAPDEFARRREARELKVSRRLFVFGTLAASLAVAALLISLFTLWQRNN
                     RLQDELARLSADANRSAQELARLRADRELLAAPEAHTATLAGTELATRARARLTFDER
                     TGRAMLMAADLPPAPAGKAYQLWFIAAGKPPMPGSVFQTDASGHAEMHDIVPPEGRRA
                     AVYAVTLEPASGTSAPTGQMYLKSSAS"
     gene            complement(1415..2002)
                     /locus_tag="VGB76_08845"
     CDS             complement(1415..2002)
                     /locus_tag="VGB76_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0099046.1"
                     /translation="MAEAAPQASSDTELLHAVARGDESALAALYDRYNSILLGLLLRI
                     LHSRVEAEDVLQEVFIQIWQRAANFDEARGRGFTWMVTLARSRAIDRLRSLQSRQRAD
                     DTALRDAPEAVGDASDDAYHAEQREIVRGALAQIPEEQRRALLLAYFEGLTQSEIAAR
                     LGQPLGTVKTRMRSGMSKLRELLDERMGRGAKLNF"
BASE COUNT          381 a          629 c          748 g          449 t
ORIGIN      
        1 gagtgacgag caagaacaag ttaaaagtgt cgccgtcatt tgtgactgtc gcgtgcgtgg
       61 cgggcgacga gagaaaaaag tttgaagcca ggattgctgc tttaacttgt cactcgtcac
      121 ttatcactcg tcactcgtca cttcaacttg ctgtctttga gtttgttcca cttgtcgcgg
      181 atgtcgccgg tgaggtcgct ggggagggtt tcgacgggca cgtcgttttc ggcgcagaag
      241 gtgcgccaga ggagaaatcg cgcgtcgaat tgtcccgtct cgtcggtaat cgtgttcatc
      301 acggcatcgt tatcagtgcg tttttcgtca gccactttcc atccctctcg tacatgaaat
      361 tctcgcgcgc ccgcgtagtc ggagatacta taacaatttt ttcgtctccg ggtgattaac
      421 gggcggtgcg cggcgcgcaa tttaaagagt tgtctgagcg ggagttcagg aggcggagct
      481 tttcagatac atctgcccgg tgggcgcgct cgtgccgctc gcgggttcga gtgtgacggc
      541 gtagacggcg gcgcggcgtc cttcgggcgg aacgatgtcg tgcatctctg cgtggccgct
      601 ggcgtcggtc tgaaagacgc tgccgggcat cggcggcttg cctgcggcga taaaccagag
      661 ttggtaggct ttgcccgcag gtgcaggcgg gaggtcggcg gccatgagca tcgcgcgtcc
      721 ggtgcgctcg tcgaaggtga gacgggcgcg tgcgcgtgtc gccaactccg tcccggcgag
      781 cgtggcggta tgcgcttcgg gcgcggcgag cagttcgcgg tcggcgcgca ggcgcgcgag
      841 ttcttgcgcg ctccggttgg cgtcggcgga gagccgcgcg agttcgtctt gcaggcggtt
      901 gttgcgctgc cagagcgtga agagcgagat gagcagcgcg gcgacggcga gcgaagcggc
      961 gagcgtgccg aacacgaaga gtcggcggct gactttcaac tcgcgcgcct cgcgccgccg
     1021 ggcaaactca tcaggcgcgc tcacgttgga cgctacagcg gcggttgacg tagcagctgt
     1081 tgtcgttgcg ttgggcgcgc cgttgccgga agaggcggga aggcgcgcgg cctcctcgcg
     1141 ctcgtcctgc ggctgcgact tgatgcgtga gagaatctgc gcgcggagtt ccggcgcagg
     1201 ggcgaccggc gaagtcgcgt agatgagaga ggcggctgcg gcgcgctctg cgtcccacgc
     1261 ggcgcggcag tcggcgcact ccgcgagatg ctcggcgagc gcgtgccgct cgccctcatc
     1321 atcgagagcg tcgagcgcag cgagcgcgat caactctttg tagtcttcgt gcttcatcga
     1381 aaactcacgc gggcatcgac tcaaaaattc agactcaaaa attcagtttc gcgccgcgtc
     1441 ccattcgctc gtccaacagt tcgcgcagtt tgctcatgcc gctgcgcatg cgcgtcttga
     1501 ccgtgccgag cggttgaccg agccgcgcgg cgatctcgga ctgggtcaac ccttcaaagt
     1561 atgccagcag cagggcgcgt cgctgctctt cgggaatctg tgcgagcgcg ccgcgcacga
     1621 tctcgcgttg ctccgcgtga taggcgtcgt cgctggcgtc gcctacggct tcgggcgcgt
     1681 cgcgcaaggc cgtatcgtct gcgcgctggc gtgattgcag ggagcgcagg cggtctatgg
     1741 cgcggctgcg tgcgagcgtc accatccacg taaaaccgcg cccgcgcgcc tcgtcgaaat
     1801 tggcggcgcg ctgccagatt tggatgaaga cttcctgcaa aacgtcttcc gcttcgacgc
     1861 ggctgtgtaa tatacggagg agaagcccta agaggatgga attgtaacgg tcgtaaagcg
     1921 cggccagcgc agactcatcc ccacgcgcga cggcgtggag gagttccgtg tcggaagagg
     1981 cttgcggggc tgcttcggcc atagaggaag agatgatgaa cgcggaacga tgaacgacaa
     2041 acaggagtcg gaattcagga gtcggaattc aggagccgga agtcagaata aaacctgcgc
     2101 gagtcttcat tctgactcct gaattctctc ttctagttca tcgttcccct ccaacgctat
     2161 ctcaggcgac ggtgatctct tccgacaggt atacgtcctg aatcgcg
//
LOCUS       DASWNS010000250         4736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_61332, whole genome shotgun sequence.
ACCESSION   DASWNS010000250 DASWNS010000000
VERSION     DASWNS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61332"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VGB76_08850"
     CDS             <1..1032
                     /locus_tag="VGB76_08850"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099047.1"
                     /translation="VGAGTLLDGDPLNNYLKRALTWNEGGVLDGANNIQIAGTYAYIT
                     TDKSLVIVNIDDPLQPKVLKTIPLHHPHAVAIQFRYAFVVDGEGFKVVDVTKPEEAHL
                     VEGAEVKLAHAHDVYVARTYAYVANGKDGVAIINVERPEQPKLDQMYNAEGKLNDVHQ
                     VKVAMTNASLYGYVADGHNGLRILQLTDPETSVTYAGFSPRPDPKLIATFHTTGPAVA
                     VSKGLDRDRAVDESGNQLAVFGRRGARPFNLEELQRMYLMDGKLYTVTDNPTTPANDF
                     KGTTAKGEGDQPEAKPEKSVSANPVSKGRSIFAFREMNGTTKLIGGGLGLLFVGLGVR
                     RRRRSRRRA"
     gene            complement(1088..1966)
                     /locus_tag="VGB76_08855"
     CDS             complement(1088..1966)
                     /locus_tag="VGB76_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0099048.1"
                     /translation="MAAVTQRLPENVAGDFYVDASCIDCDACRQIAPETFRDHGEQSS
                     VYHQPETADETHRALMALVACPTASIGTLRHHNVRAAIDAYPSRISENVYFCGFTSEA
                     SFGAWSYLIVRPEAEGGNVLVDSPRFASQLVKRIEALGGVRQMFLTHADDIADHAKFA
                     RRFGCTRIMHEADGARARGIERVIEGEGEVALDGELRIIPVPGHTRGHLVLLYRDKYL
                     FTGDHLAWSPVRETLTAFRSVAWFSWKEQTRSMERLLGKRFEWVLPGHGRIHEDSPDA
                     MQAHLRRCIDWMQKTK"
     gene            complement(2085..2240)
                     /locus_tag="VGB76_08860"
     CDS             complement(2085..2240)
                     /locus_tag="VGB76_08860"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HEY0099049.1"
                     /translation="MSTILIIILIILLLGAFPRWPHSRNWGYFPSGTLGIILLIIIIL
                     VLMGRFR"
     gene            complement(2378..3721)
                     /locus_tag="VGB76_08865"
     CDS             complement(2378..3721)
                     /locus_tag="VGB76_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0099050.1"
                     /translation="MKLKTGLVAALLVPVIVLAASGQTGRGERPSVNATSVPASTAAA
                     TREERIAQAAEALRPQLIAQRRDFHAHPELSNREARTSRIVAERLKSLGFDEVRTNVA
                     RHGVVGLLKGTKPGGVVAVRADMDALPIQETNDVPYKSQTPGVKHACGHDVHTTVELG
                     VAEVLSKMRGEISGTIKFIFQPAEEGAPAGEEGGAPLMIKEGALENPRPQAIFGLHTE
                     PNVQVGQIGYHSGAAMASSDTFTINIRGKGAHGAQPHAGVDAVVVASESVLALQHIRS
                     RRIDPLEPLVITIGTIQGGTRFNVIANEVRLTGTMRTHNDKVRERAIELMRETLTHVT
                     AAYGATFELNMEGSNPVTYNEPSLVEATLPTIRRVVGAENALVLKPFMPAEDFSYFQK
                     VVPGFYFFLGVGNRAKGITAGWHTPDFDVDEESLVVGVKVMSNVLLDYLDRHKGK"
     gene            complement(4081..4736)
                     /locus_tag="VGB76_08870"
                     /pseudo
     CDS             complement(4081..4736)
                     /locus_tag="VGB76_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
BASE COUNT          879 a         1488 c         1444 g          925 t
ORIGIN      
        1 gtcggcgcgg gcacgctgct cgacggcgac ccgctcaaca actatctcaa gcgcgccctg
       61 acgtggaacg agggcggcgt tctggacggc gcgaacaaca tccagatcgc gggcacctac
      121 gcctacatca cgaccgacaa gtcgctcgtg atcgtcaaca tcgacgatcc tttgcagccg
      181 aaggtcttga aaacgatccc gctgcatcac ccgcacgccg tcgccataca gtttcgctac
      241 gccttcgtcg tagacggaga gggcttcaag gtggttgacg tgacgaagcc ggaagaggcg
      301 catctggtgg aaggcgcgga agtaaagctc gcgcacgcgc acgacgttta tgtggcgcgc
      361 acttacgctt acgtcgccaa cggcaaagat ggcgtcgcca tcatcaacgt cgagcgaccg
      421 gagcagccga agctcgacca gatgtacaac gccgaaggca aactaaacga cgtgcatcag
      481 gtgaaagtgg cgatgacgaa cgcgagcctc tacggctacg tggccgacgg gcacaacggc
      541 ctgcgcatcc tgcaactgac cgacccggaa acctccgtca cctacgccgg attcagcccg
      601 cgccccgacc cgaaactgat cgccacgttc cacacgacgg gtcccgccgt cgccgtctcc
      661 aaaggactcg accgcgaccg cgccgttgac gaaagcggca atcaactcgc cgtcttcggc
      721 aggcgcggcg cgcgcccctt caacctcgaa gagttgcagc gcatgtatct catggacggc
      781 aagctctaca ccgtcaccga caacccgacg acgcccgcca acgatttcaa gggcacgacg
      841 gcgaagggcg aaggcgacca accggaagcg aaacccgaaa agagcgtctc cgccaatcca
      901 gtctcaaagg gcaggtcaat cttcgcgttc cgggaaatga acggcacgac caaacttatc
      961 ggcggaggat tgggattgtt gttcgtcgga ttaggcgtgc ggaggcgacg aagatcgagg
     1021 cgacgggctt aaactgcata gaacctgaaa cgaaacagcc ctgccatgtt agagaaacgt
     1081 ggcggggcta tttcgtcttt tgcatccagt cgatgcagcg tctgaggtgc gcctgcatcg
     1141 cgtcgggcga gtcttcgtga attcgcccgt ggccgggcag cacccactcg aaacgcttcc
     1201 ccagcagtct ctccatcgag cgcgtctgct ccttccacga aaaccaagcg acgctgcgaa
     1261 aagccgtcag cgtctcgcgc acgggcgacc acgccagatg atcgcccgtg aagagatact
     1321 tgtcgcgata gagcagcacg agatggccgc gcgtgtggcc gggaacgggg atgatgcgaa
     1381 gctctccgtc gagcgcaacc tccccttccc cttcgatgac gcgctcgatg cctcgcgcgc
     1441 gtgcgccgtc cgcctcgtgc atgatgcgcg tgcagccgaa gcggcgtgcg aatttcgcgt
     1501 ggtcggcgat gtcgtcggcg tgcgtgagaa acatctgccg cacgccgccc aacgcttcga
     1561 tgcgtttgac gagttgcgaa gcgaaacgcg gcgaatcgac gagcacgttt ccgccctcag
     1621 cttccggtcg cacgatcagg taactccacg cgccgaaact cgcttcggaa gtgaacccgc
     1681 agaaatagac gttctctgag atgcgcgacg ggtaggcgtc gatggcggcg cgcacgttgt
     1741 ggtgccggag cgtgccgatg gaggcggtgg gacaggcgac gagagccatc agggcgcggt
     1801 gcgtctcgtc cgccgtctcc ggctggtgat agacgctcga ctgctcgccg tgatcgcgaa
     1861 acgtctccgg cgcgatctgg cggcacgcgt cgcaatctat acaactcgcg tcaacgtaga
     1921 aatcgcccgc gacgttctca ggcaagcgtt gggtgacggc ggccatgcgt gtagtttcag
     1981 gtttcccgtg gaaagtttca agtcaggaca caacggcagc cacccgtctg cctccgaccc
     2041 cgaaactcga cgcacgcagc gcaaacctga aacgcgaaat tgtcttatct gaaccgaccc
     2101 atcaacacga gtatgatgat gatcaggagg atgatgccga gcgtgccgct tgggaagtat
     2161 ccccagttgc ggctgtgcgg ccaacgcgga aatgcaccga gcagcaggat gatcaggatg
     2221 atgatcagga tcgttgacat aacagtttcc ctccctcaaa agttacccgc gcaagttgtc
     2281 ggaactggcc ggagcgagcg ttgaaatgga ctccggtgtg acggccttga ggttatgccc
     2341 cgcgcggttt caggtcaatc tttttattcc gcgttcatca tttccctttg tgccggtcga
     2401 gataatcgag cagcacgttg gacatgactt tgacgccgac gacgaggctc tcttcgtcca
     2461 cgtcgaagtc gggcgtgtgc cagcccgccg tgatgccttt cgcgcggttg cctacgccga
     2521 gaaagaaata gaagccgggc acgaccttct ggaagtagga gaaatcttcg gctggcatga
     2581 agggcttgag cacgagcgcg ttctccgcgc ccaccacccg gcggatggtc ggcagcgtcg
     2641 cttcgaccag cgaaggctcg ttgtaggtga ccggattgct gccctccata ttcaactcga
     2701 acgtcgcgcc gtaagcggcg gtgacgtgcg tgagcgtctc gcgcatcaac tcgatggcac
     2761 gctcgcgcac tttatcgttg tgcgtgcgca tggtgcccgt cagtctcact tcgttcgcga
     2821 tgacgttgaa acgcgtgccg ccctgaatcg tgccgatggt gatgacgagc ggttctaacg
     2881 gatcgatgcg gcggctgcgt atgtgttgca gcgcgagcac gctttcggag gcgacgacca
     2941 cggcgtccac gcccgcgtgc ggctgtgcgc cgtgtgcgcc cttgccacga atgttgatgg
     3001 tgaaagtgtc ggatgaggcc atcgccgcgc cggagtggta accgatctgc ccgacctgca
     3061 cgttcggttc ggtgtggagg ccgaagatgg cctgcgggcg cgggttctcc agcgcgcctt
     3121 ctttgatcat caaaggtgcg ccgccctctt cgcccgcggg cgcgccctct tccgccggtt
     3181 ggaagatgaa cttgatcgtg ccgctaatct ccccgcgcat cttgctcagg acttcggcca
     3241 cgcccaactc gacggtcgtg tgcacgtcgt ggccgcaggc gtgcttgacg ccgggcgtct
     3301 gcgacttgta aggcacgtcg ttggtttcct gaatcggcag cgcatccatg tccgcgcgca
     3361 cggccaccac gccgcccggc tttgtccctt tcaacaaccc gacgacgcca tgccgcgcta
     3421 cgttcgtacg cacctcatcg aacccgagcg acttcaaacg ttcggcgacg atgcgcgacg
     3481 tgcgcgcttc gcggttggag agttcggggt gagcatgaaa gtcgcgccgc tgtgcgatga
     3541 gttgcgggcg cagcgcctcc gccgcctgcg cgatgcgctc ctcgcgcgtg gcggcggcag
     3601 tagaggcggg gacagaagtg gcgttgacag aaggccgctc ccctcttccc gtctgaccgc
     3661 tcgcggccag aacgatgacg ggcacgagga gcgcggcaac gagtccggtt ttcagtttca
     3721 tctttttcat gtgcgacgct tgtgctccga cctgcaagtt caagctcatc aaactatcaa
     3781 tcctgcaacg cgctttgcct ccagacttcg cccgctccgc ctctttgaaa cttgctgccg
     3841 tatatttctc gcatactaca tcaccggacg cccgcgcaat aaactttttc tttccccccc
     3901 ggcgcaaaaa gcggagggcg gagccgcttg ccagcgaccc cgccctcttt tgatttgtag
     3961 atttaactac ttgcccgtgt taacaaactg gatgcagtcg ccctgattct tgaatctggg
     4021 cgagccgctg cgccagccgt tgtttttgca ttggtctttg gtagtcgggg ctgcgtcgcc
     4081 gcaggcatcg ccgacgccgt cgccgtcaaa atcttcctga ccggggttgg ccgtgagcgg
     4141 gcagttgtcc gtctcgtccg cgacgccgtc gttgtcgtcg tccccgtcgc acacgtcgcc
     4201 cgcgccgtca ttgtcgttgt cggcctgatt gggattcgcg tcgctcacgc agttgtcata
     4261 accgtcgccg atgccgtcgc cgtccgtgtc ggcttcgaag tcgtaggtca cgcagttgcc
     4321 gttgtgggcg atggtgagcg tatcgacgtt ggcgttcagg ccggggtttc cgctgccctg
     4381 attgatcccg atcccgccga ggatgatggc gtcggggttg ttcgccacga tctcgctcca
     4441 ggtcttatcg cacgccgccg tcgcgcccac gcattggccg ttgatggctc tcgtagacca
     4501 ccagatgccg ctccccaacg cgtcccaatt ctgccacgta ccgttcacga cgggctgctg
     4561 gttcaggttg taaaccggct caaagaccag cgtggcaaat ccgcctgcga cattcgggcc
     4621 gttgaagtcg atcaccatgt tgagtgcggg cagttggtcg ccccctccgg cggtgcggta
     4681 agtgtcgtag ctcaactggg tgaggtcagc cagccgggtg ccttcgtgtt catagt
//
LOCUS       DASWNS010000251         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_193246, whole genome shotgun sequence.
ACCESSION   DASWNS010000251 DASWNS010000000
VERSION     DASWNS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193246"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VGB76_08875"
     CDS             complement(<1..512)
                     /locus_tag="VGB76_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099051.1"
                     /translation="MNRRTRRMNFILSVLPALLCAFVVSTLIPVSAHAAAEKAIKKDD
                     ARKLIAALSIFELNKEAVTIREISPPGAVATVLASVKLGFRFVKDEAGVWRVADVRTG
                     DRQWESFELLARALGDENISQVRAELDALAAELDALAGKSKGKAATDAPTAESRSAND
                     AQEQASKELKR"
     gene            complement(577..978)
                     /locus_tag="VGB76_08880"
     CDS             complement(577..978)
                     /locus_tag="VGB76_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0099052.1"
                     /translation="MSEQTSQTRRRILVADDDPAILRLVKTLVEKEGYTVVTASDGRE
                     AYKILQTDTDFTAAIFDVVMPHIQGPELVRHMKTEKRLMKIPVMMMTAEQNPRHSSES
                     FGAGAIVFLPKPFTASQLQVMLRVLISNAGS"
     gene            complement(1057..2145)
                     /gene="pdxA"
                     /locus_tag="VGB76_08885"
     CDS             complement(1057..2145)
                     /gene="pdxA"
                     /locus_tag="VGB76_08885"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY0099053.1"
                     /translation="MSENRHSLSAAESASRAGVGGGRRRPRIGVTIGDPAGIGPEVVL
                     KAVAESAVRDVCAPVIIGDAQLLSHTARKLDLQCGYDVIRRDEPLPDHLDSPVIYHLD
                     NIRADIEPGMESSAAGRASGEYIEAAVELCMRGTLDAISTAPINKRALFLGGYSFPGH
                     TEFLAHLTHTPECAMAFVAANLRIVLISTHVPLAEAIRLVRRERIEPVVRLANRELKR
                     WGISEPRIALAALNPHGAEGGLFGVEESAEMVPAVEACRELGINVTGPFSADTVFLRA
                     SRGEFDAVVACYHDQAMIPVKCLSFGEAVNVTLGLPFIRTSVDHGTAFDIAGRGLAEH
                     SSMVAAITLAAELSRTEDSNQEAVTSDQ"
     gene            complement(2248..2742)
                     /locus_tag="VGB76_08890"
     CDS             complement(2248..2742)
                     /locus_tag="VGB76_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099054.1"
                     /translation="MNRKLIRPTLADVKNREPNPQRERNDRYEGGGGASREGASRTGG
                     SGSGSSGAPPHQSRPPQRDSGDRNSASSAAAQGRKRIPPSETNAEIFYYKKQMDAHTQ
                     MVIVLNDGEEIIGTIEWYDRAALKINRRDEPNILLLKHNIKYMFKAEDRDGGGDEDDA
                     GDDS"
BASE COUNT          578 a          922 c          905 g          652 t
ORIGIN      
        1 cgcttcagtt ccttcgacgc ttgctcctgc gcatcgttcg cgctccggct ctccgccgtc
       61 ggggcatcgg tcgcggcttt gcctttgctc ttcccggcga gcgcgtcgag ttcggcggcc
      121 agagcatcga gttcggcgcg cacttgcgag atattttcat ccccgagcgc acgcgcgagc
      181 agttcaaacg attcccactg gcggtcgccc gtccgaacat ccgccacgcg ccagacgccc
      241 gcttcgtcct tcacgaaacg aaatcccagt ttcacactcg ccagcacggt tgcgactgcg
      301 ccgggcggcg atatttccct gatcgtcacc gcctccttgt tgagttcaaa gatggaaagc
      361 gcggcgatca acttgcgcgc gtcgtccttc ttaatagcct tctctgcggc ggcatgtgca
      421 gagacgggaa taagggttga aacgacaaac gcgcagagca gcgcggggag gacgctcaag
      481 ataaaattca tccggcgtgt gcgtctgttc atacgtctgc tcgtgcgcgg ggaatggcgt
      541 tcggcggtga aagtcgaagc gtatgcggcg gcaatttcaa ctgcccgcgt tgctgatcaa
      601 gacgcgcagc atgacctgta gctgcgatgc ggtgaaaggt ttcgggagaa acacgattgc
      661 gcccgcgccg aaactctcgg aggagtgtcg cgggttctgc tcggcggtca tcatcatcac
      721 cggaattttc atcaggcgct tctcggtctt catgtggcgg acaagttccg gtccctgtat
      781 gtgcggcatc accacgtcga agatggcggc ggtgaagtcc gtgtcggtct ggagaatctt
      841 ataagcctcg cgcccgtcgc tggccgtcac caccgtgtag ccctcttttt ccacgagcgt
      901 tttgaccaga cgcaagatcg ccggatcatc atccgccacc aaaatgcgtc gccgcgtttg
      961 tgaagtttgc tctgacatct ttttaagtga ccagtgacga gtgaccggtg acgggcaaga
     1021 atcaaaacaa gtttttaatt ttcaacttgt cactcgtcac tggtcactcg tcactgcttc
     1081 ctgattgctg tcttccgtgc gcgaaagttc ggcggcgagc gtgatggccg caaccatgct
     1141 ggaatgttcg gccagcccgc gccccgcgat gtcgaaggcc gtcccgtgat cgaccgaggt
     1201 gcggatgaac ggcaatccca acgtgacgtt gaccgcttcg ccgaacgaga gacacttgac
     1261 cggaatcatc gcctgatcat gataacaggc caccaccgca tcgaactcgc cgcgtgaggc
     1321 gcgcaagaat acagtgtccg ccgaaaaagg ccccgttaca ttaatcccca actcgcggca
     1381 cgcttccacc gcaggcacca tctccgcaga ctcttcgacg ccgaacagac cgccctccgc
     1441 cccgtgcggg ttgagcgcgg ccagagcgat gcgcggctcg ctgatgcccc aacgcttgag
     1501 ttcacggttc gccagtcgca ccaccggctc gatccgctcg cgcctgacga gccggatcgc
     1561 ttcggccagc ggcacgtgcg tcgagattag cacgattctc aagttggccg cgacaaaggc
     1621 catcgcacat tcgggcgtgt gcgtgaggtg cgccagaaac tccgtgtggc cggggaaact
     1681 gtagccgccc agaaacaacg cacgcttgtt gatcggcgcg gtcgagattg catcgagcgt
     1741 tccgcgcatg cacagttcga ccgccgcttc gatgtactcg cccgaagcgc gaccggcggc
     1801 ggaagactcc atgcccggtt cgatgtcggc gcgaatgttg tcgagatgat agatgacggg
     1861 ggagtcgaga tggtcgggca gaggttcgtc gcggcggatg acatcgtagc cgcattgcag
     1921 gtcgagtttg cgcgccgtgt gcgagagcag ttgcgcgtcg ccgatgatca cgggcgcgca
     1981 cacgtctctg accgcacttt cggccaccgc cttgaggacg acttcggggc cgatgcctgc
     2041 cgggtcgcca atcgtcacgc cgatacgagg gcggcgtctg ccgccgccga cgccagcgcg
     2101 cgaggccgac tcggcggcgg ataagctgtg cctgttctcg ctcatttttt cgctcatctc
     2161 acgatgcaaa aatgagcggg cgcggctgtt gagcatcaag agccgcgcgc ccgcgtgagc
     2221 tgcaaaagag aatcaactaa cggagggtta gctgtcatcc cccgcgtcgt cttcgtcgcc
     2281 gccgccgtcg cggtcttcgg ctttgaacat gtacttgatg ttgtgcttga gcagcagaat
     2341 gttcggctcg tccctgcgat tgattttgag cgcggcgcga tcataccact cgatagtgcc
     2401 gatgatctct tcgccgtcgt tcaggacgat caccatctgg gtgtgggcgt ccatctgctt
     2461 tttgtagtag aagatttcgg cgttcgtctc agagggcgga atccgcttgc gcccctgcgc
     2521 ggcggcagac gaagcggaat tcctatcgcc ggaatctctt tgcggcggac gcgactggtg
     2581 cggaggcgca ccgctgctcc cgctgccgct acctccagtg cgcgacgctc cttcgcgcga
     2641 cgcgccgccg ccaccctcat acctgtcgtt gcgctctctt tgcgggttcg gctcgcgatt
     2701 cttcacatcg gccaatgtcg gtcgtataag tttgcgattc acttttttac ttagcctcca
     2761 acagtctgac aagcaggaaa acggcgcgcg tgcatttaaa taccccgact ctaaagcgga
     2821 cgagttgcca aacaaatggc gcgcttgggt gcgcctgttt ttacaggcgg tggtcgtttg
     2881 caagacgaga aacgtgttgt ctcgttattt gaattacaat cctgtcagga atgggcgaca
     2941 tgctacacga ccacctcttg tttggcaagc caatgaagac ggtgacgagt aacgagtgac
     3001 aagtgacaag ttaaaagacg ggtgacgagc taaaacaggt attctgtttt aactcgt
//
LOCUS       DASWNS010000252         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_139310, whole genome shotgun sequence.
ACCESSION   DASWNS010000252 DASWNS010000000
VERSION     DASWNS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139310"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..602)
                     /locus_tag="VGB76_08895"
     CDS             complement(177..602)
                     /locus_tag="VGB76_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY0099055.1"
                     /translation="MGTKLYVGNLSFRTTSDELKDLFSQAGEVESASVIEDRETGRSR
                     GFGFVEMATSEGGAAAITQFNGYELGGRALTVNEARPKADRGGGGGGGGRGGYGGGGG
                     GGRGGYGGGGGSGYGGGGGGRDRGDRGGGGGGGDREPRW"
     gene            1174..4194
                     /locus_tag="VGB76_08900"
     CDS             1174..4194
                     /locus_tag="VGB76_08900"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HEY0099056.1"
                     /translation="MIGQTISHYRILDQIGEGGMGVVYEAEDTRLGRRVAIKIPSAAP
                     DSHSHHARFLREARSISALSHAHIATLFDYGETPDGRPYIVMELVNGRELGDLLRNEE
                     LSIPRAIEIIADVGEALCEAHRHGIIHRDIKPSNVIINERGDVKVLDFGLAKLIGEEQ
                     EVDQNAATLAGVKTRSDVMLGTPLYLSPEQARALPVDGRSDLFSLGALLYECVAGRPA
                     FSGATVVEIAAQVIHVNPEPPSKFNRLIPPELDRITLKAISKHPEDRYQTAEEFVADL
                     RALGFHFSELDDTRTPSMTSYSSGAYHTNAHRATSLTTLSDNLRRPRLSIATVIIVVA
                     VLMGGTWGANRLLRPTPHMPSAPAQAEYDLGTNLLRAGAYYQASTHLRYATELDDKFV
                     LAHARLAEALTELDNDDDAKDSMLRIDSHAVERDNLSQVDTLRLDAIRATITRDYARA
                     VRAYQEIARLLPDRADTYVDLGRAHEKMEQSKLAIQSYVEATNRDPKQATAYLRIGIL
                     YARNGNTPSANAALDTAEKIYKEFANNEGQTEVLHRRGSLLRAIGQLDEARTQLEQAL
                     EMAKAGGNVAQRINTLLQLSAVATNKNDAAGAQRYAREAVELAQKNDMGNLVALGNVD
                     LGLTFQAKDKYAEAEQYLRQGLDFARRYNARRAEAKALGNLGSVYIKQGKTDEGIRYV
                     EQARAFYQAGGYRKEEAQALAMLGRAKRQKGNYAEALADCQQVLQLSEQVNDPTLISA
                     AHVELGNVLAHQENYIDALHHFEKSYQIQKSLGLEIYAGYSLHNRSEMLWRLGRYQEA
                     REMLSQALAIAEKPGKQHEELLAHSYLTQAQIALSESRYGEAQAKSEQALNAAGDSNK
                     SLAVEAKRTLGLAQSSSGATAKGKLWCEEALATAKLTGDPWLISCSVLALAKAQLAHG
                     EAAAARESAQEAQASFARAGQKVSEWRAWFVAARASQREGDPAAARDYAARATNLLAE
                     LQQTWGAAAFEVYQARPDVQLCRKQLTELSAGVR"
BASE COUNT          939 a         1400 c         1267 g          716 t
ORIGIN      
        1 acgagcgacg agtgacgagt gacaagttaa aacagtgacg aatgaagaga agcgcgacga
       61 gcgataggaa cgccaaagtg aagagtggca ggagcgcaaa aagcgacgag tgacggttga
      121 aagacaaagg gcttttgtct cgtcactcgt cactcgtcac ttgtcactgt tttgagctac
      181 cagcggggtt cgcggtcgcc gccaccaccg ccgccgccgc gatcaccacg gtcgcggcca
      241 ccgccgcctc cgccgtagcc gctgccaccg cctccgccat agccgccacg accaccaccg
      301 ccaccgccgc cgtaaccgcc gcgcccgccg ccgcctccgc caccgccgcg atcagctttg
      361 gggcgtgctt cgttaaccgt cagggcacgg cctcctagtt cgtagccgtt gaactgcgtg
      421 atggccgccg cgccgccttc cgatgtcgcc atttcgacga atccgaagcc gcgcgaacga
      481 cctgtttcgc gatcctcaat cacggatgcc gactccactt cgcccgcttg cgagaacagg
      541 tcttttagtt cgtcgcttgt ggtgcggaag gagaggttcc ccacgtaaag tttcgtaccc
      601 atatcttttt tccttaaaag tcgaaggaag cgcgggcgtc taacagatca agctggccga
      661 agtctatgga agctctggca aacggctcgg cggcgacgcg cccgctgctc taacactagc
      721 gagagcggct tctaacaaat tgacaatcca acctcactct cttcagccga acgccgtcgt
      781 cgcctaactt tcgtcgcagg caggcacatc atcaccgtca gatgcgcgag agcaggaact
      841 ttgaacgggc aaaagtatgc ccgcaatcga gtcgaagcgc aagagataag tttggccgcg
      901 cgccgctgcg ccgggcgtaa aattcccaac gaaagggcga cccgaacaag tggaagggcg
      961 aacctcgcct ccacgcctct cgtagatcag gtttgggcag cgaaagaacc ttcgcctaat
     1021 tttgcccttg aaatgcttga tgctttgtcg cagaatacgc ggcagacgaa caaacgagtt
     1081 tttctaacgc actccaactg ccgcattggg tactaggttc gttttgttca cgccaactgc
     1141 tgcccaggtt cggggctaaa ggaatccgtt tttatgatcg ggcagaccat ttcccattac
     1201 cgcattcttg atcaaatcgg cgagggcgga atgggagtgg tctatgaagc ggaagacacg
     1261 cgcttggggc gacgcgtcgc catcaagatt ccttctgccg cgcccgacag ccacagccat
     1321 cacgcccgct tcctgcgcga ggcgcgctcc atctccgcgc tcagccacgc gcacatcgcc
     1381 accctcttcg attacggcga gacgcccgac gggcggcctt acatcgtgat ggaactcgtc
     1441 aacgggcgag aactcggaga cttgctgcgc aacgaagagc tgtccatccc ccgcgccatc
     1501 gagatcatcg ccgacgtggg cgaagccctc tgcgaagctc accgccacgg catcattcat
     1561 cgcgacatca aaccctcgaa cgtgatcatc aacgagcgcg gcgatgtcaa agtgctcgac
     1621 ttcggactgg ctaaactcat cggcgaagag caggaggttg accagaacgc cgccacgctc
     1681 gccggagtca aaacgcgcag cgacgtgatg ctcggcacgc cgctctacct ctcgcccgaa
     1741 caggcgcgcg cgctgcccgt cgatggacgc agcgatctct tcagcctcgg cgcgctgctc
     1801 tacgagtgcg tcgccggtcg tccggcgttc tccggcgcaa cggtcgtcga gatcgcggcg
     1861 caggtcatcc acgtcaatcc ggagccgcct tcgaagttca accggctcat cccgcccgaa
     1921 ctcgaccgca tcacgctgaa agcgatctcc aagcacccgg aggatcgtta ccagacggcg
     1981 gaagagttcg tcgcggattt acgcgcgctc ggtttccact tctccgagtt ggacgacacg
     2041 cgcacgccga gcatgacctc ctattcctcc ggcgcgtatc acaccaacgc tcatcgagcg
     2101 acttcgctca cgacgctctc ggacaacctg cgccgcccgc gcctctctat cgccacggtg
     2161 atcatcgtcg tcgcagtgct gatgggcggc acgtggggcg cgaaccgctt gctgcgcccc
     2221 acgccgcaca tgccttccgc cccggcgcag gcggaatacg acctcggcac gaatctgctg
     2281 cgcgcgggcg cgtactatca ggccagcacg cacctgcgtt atgccacgga actcgacgac
     2341 aaattcgtgc tggcgcacgc gcgtctggcc gaagcgttga ccgaactgga caacgacgac
     2401 gacgcgaagg attcgatgct gcgcattgat tcgcatgccg tcgaacgcga caacctctcg
     2461 caagtggaca cgctccggct cgatgccatc cgcgccacga tcacgcgcga ttacgctcgc
     2521 gccgtgcgcg cttatcaaga gatcgcgcgc ctgttgcccg accgcgcaga cacctacgtc
     2581 gatctcggtc gcgcccacga gaagatggag cagagcaagc tggctatcca gagttacgtc
     2641 gaagcgacga accgcgatcc gaaacaggcc acggcctacc tgcgcatcgg catcctctac
     2701 gcgcgcaacg ggaatactcc cagcgccaac gccgcgctcg acaccgccga aaagatctat
     2761 aaagaattcg ccaacaacga aggccagacc gaagtgctgc accgtcgcgg ctctctcctg
     2821 cgcgccatcg gacaactcga cgaggcgcgc acgcagttgg agcaagctct ggagatggcg
     2881 aaggcgggcg gcaacgtcgc gcagcgcatc aacacgctct tacagttgag cgcggttgcc
     2941 accaacaaga acgacgcggc gggcgcgcaa cgctacgcgc gcgaagccgt cgagttggcg
     3001 cagaagaacg acatgggaaa cctcgtcgcg ctcggcaacg tcgatctcgg cctgaccttt
     3061 caggcgaagg ataaatatgc ggaggccgaa caatatctga ggcagggact cgatttcgcc
     3121 cgcaggtaca acgcgcgccg cgccgaagcg aaggcgctcg gcaacctcgg cagcgtctat
     3181 atcaagcagg gcaagaccga cgaaggcatc cgctacgtgg aacaggcgcg cgccttctat
     3241 caagcgggcg gctaccgcaa agaggaagca caagccctcg ccatgctcgg tcgggctaaa
     3301 cgccagaagg gaaactatgc ggaagccctc gccgactgcc agcaagtgct ccaactctcc
     3361 gaacaggtca acgacccgac gctcatctcc gcggctcatg ttgaactggg caacgtcctc
     3421 gcccatcagg agaactacat agacgccctg catcatttcg aaaagagtta ccagatacag
     3481 aagtcgctgg ggcttgaaat ctacgccggt tacagcctgc acaaccgtag cgaaatgctc
     3541 tggcgtctgg gtcgttacca ggaggcgcgg gagatgctga gtcaggcgtt ggcgattgcc
     3601 gagaagcccg gcaaacagca tgaggaactg ctggcgcaca gttatttgac gcaggcgcaa
     3661 atcgctttga gcgagagccg ctacggcgag gcgcaggcga aaagcgagca agccctgaac
     3721 gccgcgggcg actcgaacaa gtcgctggcc gtcgaggcca agcgcacgct cggtctggcg
     3781 cagagttctt cgggcgcgac ggccaagggg aaactctggt gcgaagaggc actggctacg
     3841 gccaaactca ccggcgaccc ctggctcatc tcctgttcgg tgctggctct ggcaaaagcg
     3901 cagcttgcac atggcgaggc ggctgccgca cgcgagagcg cgcaggaagc gcaggcgagt
     3961 tttgcccgcg ccgggcagaa ggtttcagaa tggcgcgcgt ggttcgtggc ggcacgtgcc
     4021 agccagcgcg agggcgatcc ggcggcggcg cgggattacg ccgcgcgcgc gaccaattta
     4081 ctggcggaac tccaacagac gtggggagca gccgcatttg aagtttatca ggcgaggccg
     4141 gatgttcaac tttgccgcaa gcaactcact gagctatccg ccggagttcg ataaatttgt
     4201 cacttggttt aacgcgaaag gaaacggaca tgaacgatcc tgaagataac gagcaaccgc
     4261 cggagactga cgatcaatcg cctcctgcca ccgacgatac gcaggaggac acctccgacg
     4321 gt
//
LOCUS       DASWNS010000253        12202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_243949, whole genome shotgun sequence.
ACCESSION   DASWNS010000253 DASWNS010000000
VERSION     DASWNS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12202
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243949"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VGB76_08905"
     CDS             <1..597
                     /locus_tag="VGB76_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099057.1"
                     /translation="GLSIAWSVAEYLHDSPEHAAKTLFATHYHELTELAERLPGAQNY
                     QIRVAEREGEVIFLYKLERGRASKSYGIEVARLAGLPPQVLTRARDVLQRLERYELDV
                     FAETAKAAAGISQAGSAQGLEARPSEKNGGDALEKNGGDALGQAARRAGRRALAAQST
                     LFDTVNQGLLDELRSVDVENLSSEEIRTLLKNIRTRIV"
     gene            760..1899
                     /locus_tag="VGB76_08910"
     CDS             760..1899
                     /locus_tag="VGB76_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY0099058.1"
                     /translation="MTDKLTSLTLEQKIGQLLYIGLPGAEFDADTRALVADVQPGGVI
                     IFGRNVTAPEQLRRLLDSVRETVRVEPLLGIDQEGGLVDRLRRICTPMPSARTIRQHG
                     DLAAARALGKLTGEALRLLGFNMNFAPVLSIMTDDRDLLTNGLYSRSFGRSPGEVLGY
                     TMVYLRGLQGEGCLGCAKHFPGIGAGEVDSHEEMPIINLSHEDLIAQDLAPYIELFQR
                     EDNMVHAVMVSHGGFPKVDLRRGTAGGRLIPASINPQIVTRLLREELGYTGLVVTDDL
                     EMGAIARHYEIEETVRRAIEAGEDMLLICARPDLIRRGFASLLQAARDGVLTEERINA
                     SLARISHFKTLIQPPLPFDATRLAGISDAVAALNRKLNYTYGGKI"
     gene            1899..3521
                     /locus_tag="VGB76_08915"
     CDS             1899..3521
                     /locus_tag="VGB76_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0099059.1"
                     /translation="MRIGNSDGSRRERRKAMRNHKILFNSCSLLVVLCLLISGASCRR
                     AGGDAFVMVLEASAKTLNPLRGTDAASERYRQLMFNSLMRKNEQFDYVGELASNVQTS
                     ADGMSVTFTLQDNVKFHDGKQMTSADVKYTFDQLLASDSPKAVPFFEGTGDSKKPLVA
                     SVEAPDARTVVFRLNRQWLELFANLIPIAIIPEGTFETQAQKPLGTGAFKFARFDESQ
                     QVVDLEAHDNYWEGAPNIKHLRVKVLLDANTQQAELVAGRIDLAINTALSPDAYVNLA
                     KNPNLQLVQSPGVNVQYIGFNTESAPVNNPRVRQAIAYAIDRESIVKNLLQGQARIAH
                     SILPQESWAYSPEQKTYSYDPAQAKRLLDEANFPDPDGDGPKMRFEQPLSFKISASNT
                     VVRQYVGVMQNSLREVGIPVNIETLEDNTLREAQRNGQYQLVAGRWVGGNQDPIFLRD
                     LFTYLTGGNFNRGRYRNAELDEILGEAVTTADRAKARTLYAQAQDLISREMPMFPLWY
                     ANNMVIARRNIGNIKVAPGADWTFVRNLTVSK"
     gene            complement(3658..4272)
                     /locus_tag="VGB76_08920"
     CDS             complement(3658..4272)
                     /locus_tag="VGB76_08920"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY0099060.1"
                     /translation="MRDSQETRERAAALVKAGSVIAFRTDTFYGLGADPFNRDALRQL
                     RALKRRDDGKPILLVISDASEVARFSPRLGKLFDALCARHWPGALTLVVRAHADLPTE
                     LTAGTGTIGLRLPADEAVRALVRACGGALTATSANLAGEPPARAAGEVLRSFPTELAL
                     IIDGGVTQTEEASTVLDVSGDEARLLREGVVSWRVLEETITSAG"
     gene            complement(4312..5304)
                     /locus_tag="VGB76_08925"
     CDS             complement(4312..5304)
                     /locus_tag="VGB76_08925"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY0099061.1"
                     /translation="MKLLFNFVVWCALLSTFASGQQATSPQVQRQTPPAAPRSRLFDA
                     GQLLRDVETLSSEAMGGRKAGTEGGAKARDYVARRFRQSGLEFYSASPLQFFTFTEPN
                     ASGERKGANVVGYIRGKTFPHRYLVVTAHYDHLGVHNGELYNGADDNASGTAALFALG
                     KYFSKHRPAHSIIFVAFDAEEGSGGGARKFVSEPPVDIRSIILNVNLDMISRNERNEL
                     YAAGTHHYPFLKPSLEKVRAGATVKLLLGHDSPDLPPQQNWTGQSDHVAFHRAKIPFI
                     YFGVEDHRDYHRPTDDFENIQPVFYVRAVETILEAIKTFDADLTTLPTHKPVSP"
     gene            5580..5972
                     /locus_tag="VGB76_08930"
     CDS             5580..5972
                     /locus_tag="VGB76_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099062.1"
                     /translation="MRKIAAVVCLFFSLTFVVCAQDKSRAWLNGSWEGTGYQMDTATT
                     WTVNFSARGRRFSIEYPSLKCSGTWRLLRVNAQRAIFRERITVGQGECVDRGVVTIER
                     LNGRQIAYRFSYAGSTQVSASAILNRKK"
     gene            6030..7391
                     /locus_tag="VGB76_08935"
     CDS             6030..7391
                     /locus_tag="VGB76_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0099063.1"
                     /translation="MKRRLSVCLACQLLLAVSVAAQVRDKDQWPSFRGQQAAGVAEGQ
                     GLPERWDGERGANVKWKTKIPGLAHSSPVVWGDRVFVTTAVSSRGQDNFRHGLYGDGT
                     ASEDRSLHRWKLYALDKKSGKVLWERTAYEGVPREKRHIKASYANATPATNGRYVVAF
                     FGSQGLYAFDMQGRLVWKRDFGVLNAGAYDAPDYEWGTASSPIIYKEMVIVQCDTQGE
                     SFLHAASLKTGKTIWKTPRTELPSWGTPTIYPGKSRVELITNASNFIRGYDPETGKEL
                     WRLGGSSKITAPTPVFSGDLIVVASGRRPEAPIFVIRAGANGDITLPAGQSSNQHIAW
                     SKQARGSYMPTPLIYGNYLYVLSNQGVFDCYDLKTGAEIYRERIQHRGGGFSASPVAA
                     DGKIYLPGEDGDMFVVRAGAKFELLATNPMGELMMATPAISSGMMFVRTQHHLFALSR
                     TTP"
     gene            complement(7486..10002)
                     /locus_tag="VGB76_08940"
     CDS             complement(7486..10002)
                     /locus_tag="VGB76_08940"
                     /inference="COORDINATES: protein motif:HMM:NF027787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HEY0099064.1"
                     /translation="MSTSYLVKRLTSLTLTLAILLNCFAASVYPLNLPHSAHVKHASS
                     HASVAAAPSAFPALNLWNNYSATLLTLPDPTPTPTPDSKIAFTSERDGNQEIYIMNPD
                     GSQQTRLTNNSASDAEPTWSPDGTKIAFVSDRDASGGIGIGEIYVMNPYGNEQTRLTN
                     NTAFDYSPAWSPNGAKIAFVSYGSGLNHDIYVMDADGSNRTRLTTETSGDFDPAWSPD
                     GTQIVFRSYRDGSSEIFVMDSDGSDQTSLTSNNRNNSVPVWSPDGTKIAFSSDIGAQS
                     DIFLMDADGSDITRLSNDATIDHSPAWSPDGTQLSYQSYTGTAYIFDIYVIDADGSNV
                     TRLTFESSDSSASCWGFVPSSAPTPTPTPTPTPTPTPTPTPPLSNDDFADALELIGGS
                     GSVSVNNTYATGEVGEPDHAQHAAGFSVWYRWQPAAGGNVTFDTDGSTGNTCGHADTV
                     LGVYTGGSLGALTEVVSNDDVPFDVHSLVSFNATANTVYYIALDVYDASACPGALLLN
                     WQPGLPSPAPTPAADGKLAYGSYRGVTMQIFVMDGDGSAQTSLTEKTLDSDEPRWSPD
                     GTKIAFISYRDGQSEIYLMNADGSNQTRLTTSVDSYYHTWSPDSTKLAFTSYDTEYNS
                     DVFVVNADGTGLTNLSQDPTGTSYLPAWSPDGSKIAYVSSRDGDPNIYVMDADGTDRV
                     RLTTTAGDDTPVWSPDGTRIAFVRWGFNGSEIYVMDADGSDQTNVSNTGNVLPNPPFN
                     FEPDWSPDGTKLAYSSYADSSSTVHVVGADGLNHAQLTTAYDSYEPRWSPDGTQIGFI
                     VYQNGYQDIYLMEADGSLQVNLTSNSAYNGAFSWQPLPAP"
     gene            complement(10480..11049)
                     /locus_tag="VGB76_08945"
     CDS             complement(10480..11049)
                     /locus_tag="VGB76_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099065.1"
                     /translation="MLRRKKILFVFALLLLSLPGHSRAQEKSTEPRFERYAVAVWRGK
                     VMPLNLRSHALARKYRTLLGQQVKEEGVNFAGHYTLASAGCGTGCSIISIIDARTGRA
                     FFPNELTGWTGIVGDYEPPEGEEPWMYRANSRLLRVVGRPNIGAANEERYGASGIYYY
                     EWRNGRLRLVKFTHVGSYPDADPPGSRTR"
     gene            complement(11110..11667)
                     /locus_tag="VGB76_08950"
     CDS             complement(11110..11667)
                     /locus_tag="VGB76_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099066.1"
                     /translation="MRSYQKFMRRLVFIVCFACVFQAADASAIQSSQSSGRQVGGDEP
                     MLQPSDLFKDFPEIKWGMGFQDVKRAIEKTGARPVRATKDDETQLVWTGTFNSLEGRA
                     RVYFEKEAGAIDVVVGVYAYDKQKEVFETWLKKLTEKHGAAKETSDNSISVSHVWRLK
                     NGFVIELRSLKDVNSPVVDIHWVKV"
     gene            11837..>12202
                     /gene="mug"
                     /locus_tag="VGB76_08955"
     CDS             11837..>12202
                     /gene="mug"
                     /locus_tag="VGB76_08955"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEY0099067.1"
                     /translation="MPDVIAPDLRVLFCGINPGLYTAATGYHFARPGNRFWPALYTSG
                     FTRRLLSPAEEHELLTDGYGITNVVARATATAAELSAEEYVEGGRRLKAKVRRYRPKF
                     IAVLGVGAYRTAFDRPKATL"
BASE COUNT         2440 a         3611 c         3681 g         2470 t
ORIGIN      
        1 ggactctcca tcgcctggtc cgtcgccgaa tacctgcacg actctcccga acacgcggcc
       61 aagacgctct tcgccacgca ctaccacgaa ctgaccgaac tggccgagcg gcttcccggc
      121 gcgcagaact atcagattcg cgtcgccgaa cgcgaaggcg aagtcatctt cctctataag
      181 ctcgaacgcg ggcgcgcgtc gaagtcttac ggcatcgaag tggcgcggct cgcgggtctg
      241 ccgccgcaag tgctcacgcg cgcacgcgat gtcctgcaac gcctcgaacg atacgaactc
      301 gacgtgttcg ccgaaaccgc caaagccgcc gcaggcatct cgcaggcggg aagcgcgcag
      361 gggttggaag cgcgcccctc ggaaaagaac ggcggcgatg cgctggaaaa gaacggcggc
      421 gatgctctgg gacaggcggc gcggcgcgcg gggcggcgcg ctctggcggc gcagtccacg
      481 ctcttcgaca ccgtcaatca aggcttgctt gacgagttgc gcagcgttga tgtagaaaac
      541 ctttcgtccg aagaaattcg tactctcctg aaaaacatac gcacgcgaat cgtttgagca
      601 gaccacacgc cagtttgtcc tccgcgcccg gtcgccgctc gccagcgtcc ctctgccgtg
      661 ccgtctctaa ttttcgcgcg cccatcggtc atccctcttt cgcgcccacg cttgttttcg
      721 acaaaccaca gaactctctt tttcgtagta tgaaccctga tgactgacaa acttacttca
      781 ctcacgctgg aacagaagat cgggcaactg ctctacatcg gtctgccggg agccgaattt
      841 gacgccgaca cgcgcgcgct cgtcgcagac gtgcaaccgg gcggcgtcat catcttcggg
      901 cgcaacgtga ccgcgcccga acagttgcgc aggctgctcg atagcgtacg cgaaacggtg
      961 cgcgtcgaac cgctgctcgg catcgatcag gaaggcggcc tcgtggaccg cctccggcgc
     1021 atctgcacgc cgatgccctc cgcgcgcacc atacgccagc acggcgatct cgccgccgca
     1081 cgcgcgctcg gcaaactcac gggcgaagcc ctgcgcctcc tcggcttcaa tatgaacttc
     1141 gcgcctgtcc tctcgatcat gaccgacgac cgcgacctgc tcacgaacgg gctatattcg
     1201 cgctccttcg gtcgctctcc cggcgaagtg ctcggctata caatggtcta cctgcgcggg
     1261 ctgcaagggg aagggtgtct cggttgcgcc aagcattttc cgggcatcgg cgcgggcgag
     1321 gttgactcgc acgaagagat gccgatcatc aatctctcgc acgaagattt gatcgcgcaa
     1381 gacctcgcgc cctacatcga actctttcaa cgcgaagaca acatggtgca tgccgtgatg
     1441 gtgagccacg gaggctttcc caaagttgat ctcaggcgcg gcacggcggg cggacgcctc
     1501 atccccgctt cgatcaatcc gcagatcgtc acgaggttac tgcgcgaaga actcggctac
     1561 acggggcttg tcgtcacgga cgatcttgag atgggcgcga ttgcgcgtca ttacgagatc
     1621 gaggagacgg tgcggcgcgc catcgaagcg ggcgaagata tgttgctcat ctgcgcgcgt
     1681 ccagacctga tccggcgcgg cttcgcttcc ctgctacagg ccgcacgcga cggagttctc
     1741 accgaagagc gcatcaatgc ctcgctcgca cgcatctcac atttcaagac tttgatccaa
     1801 ccgccgctcc ccttcgatgc gacgcgcctt gccggaatct ccgatgcagt cgctgcgctc
     1861 aatcgcaaac tcaattacac ctatggaggc aaaatttaat gcgaatcgga aattcggatg
     1921 ggagccggag agagaggcgc aaggcgatgc gcaatcataa aattttattc aactcgtgct
     1981 ccctgctcgt cgtgctgtgc ctgttgattt ccggcgcgtc ctgccgccgc gcgggcggcg
     2041 acgcgttcgt gatggtgctc gaagccagcg cgaagacgct caatcctctg cgcggaaccg
     2101 atgcggccag cgagcgttat cgccaactga tgttcaactc gttgatgcgg aaaaacgaac
     2161 agttcgacta cgtcggcgag ttggcctcga acgtgcaaac gagcgcggat ggcatgtccg
     2221 tcactttcac cttgcaggac aacgtgaagt ttcacgacgg caagcagatg acttccgccg
     2281 acgtgaaata caccttcgat caactgctcg cctccgactc gccgaaggcc gtgcccttct
     2341 ttgaggggac aggcgacagc aaaaaaccgc tcgtcgcctc ggtcgaagcg ccggacgcgc
     2401 gcaccgtcgt ctttcgcctg aatcgccagt ggcttgagtt gttcgcaaat ttgatcccca
     2461 tcgccatcat tcccgaaggc acttttgaga cgcaggcgca gaagccgctc ggcacgggcg
     2521 cgttcaaatt cgcacgcttc gacgaatcgc agcaggtcgt cgatctcgaa gcccacgaca
     2581 attactggga aggcgcgccc aacatcaaac acctgcgcgt gaaagtcctg ctcgacgcca
     2641 acacgcagca agccgaactc gtcgcggggc gcatcgatct cgccatcaac acggctctct
     2701 cacccgacgc ttacgtcaac ctcgccaaaa atccgaatct tcaactcgtg caatcgcccg
     2761 gcgtcaacgt gcagtacata ggcttcaaca ccgagagcgc gccggtcaac aacccgcgcg
     2821 tgcgccaggc gattgcctac gctattgacc gcgagagcat cgtcaagaat ctactgcaag
     2881 gacaggcgcg catcgctcat tcgatcctcc cgcaggagtc gtgggcttat tcgccggagc
     2941 aaaaaaccta ctcttacgat ccggcgcagg cgaagagact tctggacgaa gcgaactttc
     3001 cagaccccga cggcgatggt ccaaagatga gatttgagca accgctgagt ttcaaaattt
     3061 ccgcgagcaa tacggtcgtg cgtcaatatg tgggagtgat gcagaattcg ctgcgagagg
     3121 tggggattcc ggtcaacatt gaaacgctcg aagacaacac gctgcgggaa gcgcaaagga
     3181 acgggcagta tcaactcgtc gccggtcgtt gggtgggcgg caatcaagac ccgatcttcc
     3241 tgcgcgatct cttcacatac ctgacgggcg gaaacttcaa tcgcggacgc tatcgcaacg
     3301 ccgaactcga cgagatactg ggcgaagccg tgacgaccgc cgaccgcgcc aaagccagga
     3361 cgctctacgc gcaggcgcag gatttgatca gccgcgaaat gccgatgttc ccgctctggt
     3421 acgccaataa catggtcatc gcgcgcagga acatcggcaa tatcaaggtc gcaccgggcg
     3481 cggattggac tttcgtgcga aacctgacgg tgagcaagta acgaagtaat caacactcgt
     3541 ccctcgtccc tcgtccgttg cctgtgacgc tcgcgtgcgt ggctcaagat acgcttgaac
     3601 gcctgaccac cgacaacggg cgagtgacga cgaaccactg accgctgaca gtttttttca
     3661 ccctgcgctt gttatcgtct cctccaacac acgccaactg accacgcctt cgcgtagcaa
     3721 acgcgcctcg tctccgctga catcgagcac ggttgaagcc tcttccgtct gcgtcacgcc
     3781 gccgtcaatg atgagggcga gttccgtggg aaaagatcgc agcacctctc cggctgcgcg
     3841 tgcgggcggc tctcccgcca gattcgcgct cgtcgccgtg agtgcgccgc cgcaggcgcg
     3901 taccagtgcg cgcaccgctt catctgcggg gaggcgcagg ccgatagttc ctgtgcccgc
     3961 cgtcaactcc gtgggcaagt cggcgtgcgc gcgcacaacc agcgtcaacg cgcccggcca
     4021 atgtcgcgcg cacagggcgt cgaacaattt cccgaggcgc gggctaaagc gcgcgacttc
     4081 ggaagcatcg ctgatgacga gcaggatcgg tttgccgtcg tctctccgct tcaacgcgcg
     4141 cagttgtcgc agcgcgtcac gattgaaggg gtctgcgccg agtccgtaga aggtatcggt
     4201 gcgaaaggcg atgacgcttc ccgctttgac gagtgcggcg gcgcgctcgc gagtctcctg
     4261 tgaatctcgc aaaatcattt tgacaatttc gctcctgcgg atcattaacg gtcagggcga
     4321 taccggctta tgtgttggga gcgtcgttaa gtcggcatcg aaggtcttga tcgcttcaag
     4381 aatcgtctcg accgcgcgga cgtaaaagac cggttggatg ttttcaaaat cgtcggtcgg
     4441 cctgtgatag tctctgtgat cctcaactcc gaaatagata aagggaattt tcgcgcgatg
     4501 aaaagcgacg tggtcggact gtccggtcca gttctgctgc ggcggcaggt ctggtgagtc
     4561 gtgtcctaac agaagtttga cggttgcgcc cgccctgact ttttccagag agggcttgag
     4621 aaaggggtaa tggtgtgtgc ccgccgcgta gagttcgttg cgctcgttgc ggctgatcat
     4681 gtcgaggttc acgttcagga tgatggagcg gatgtccacg ggcggctcgc tcacaaattt
     4741 acgcgcaccg ccgccgctgc cctcctctgc atcgaaggcg acgaatatga tggaatgcgc
     4801 cggacgatgc ttcgagaaat acttgccgag ggcaaacagc gcggcagtgc ccgaagcgtt
     4861 gtcgtccgcc ccgttataca gttccccgtt gtgcacgccc aagtggtcgt agtgcgccgt
     4921 gacgacgaga taacgatggg ggaaagtctt gcccctgata tacccgacga cgttggcgcc
     4981 cttccgttcg ccgctcgcgt tcggctcggt aaaagtgaaa aactgtagag gcgaagcgga
     5041 gtagaattcg aggccggact gtctgaatct tcgtgcgaca taatcgcgcg ccttcgcccc
     5101 gccctccgtg ccagccttgc gcccgcccat cgcctctgag gaaagcgtct ctacatcgcg
     5161 caagagttgc ccggcatcga acaggcggga acgcggagca gcgggcggag tctggcgttg
     5221 cacttgtggt gatgtcgcct gttgccccga cgcaaatgtc gagagcagcg cgcaccaaac
     5281 gacgaagtta aatagcagtt tcatagttat cttttacacc ttcattccgt gcgaagcggc
     5341 gtccggcaga aacggcgacg catccattct acggtttcat caactcaatt tattcagtag
     5401 agagcgtagc acgcaaggct gtagagcaag taagccgtgc gcgggttgcg tggcgggaaa
     5461 tattggcatt gaccggggcg gcgtctttcg ctaagctcac gcctcaattc ccggaagcaa
     5521 gcgacggcga cggagagttc ctgatcacac caaaattctt aaaaggaatc taagaaacaa
     5581 tgcggaagat tgctgcggtt gtctgcctgt tcttctcttt aacctttgtc gtgtgcgcgc
     5641 aggacaagag ccgtgcatgg ttgaacggca gttgggaagg caccggatac cagatggata
     5701 cggccacgac atggacggtg aatttcagtg cgcgcggcag gcgtttctcg attgagtatc
     5761 catcgctcaa gtgtagcgga acgtggaggc tcttgcgggt caacgcgcag cgggcaatat
     5821 ttagagagcg gataacggtc gggcagggtg aatgtgtgga tcgaggcgtg gtgactatcg
     5881 agcgtttgaa cgggcggcaa atcgcttaca ggttctccta cgcggggagc actcaggtat
     5941 ctgcgtcggc catccttaac cggaagaaat gaggccgccg aaattgcgtc tacgcttcaa
     6001 ggcgtaacct tgaaggggag gtcgaatcaa tgaaacgacg tttgagtgtg tgtctcgcct
     6061 gccaactctt gttggccgtc tccgtcgcgg cgcaggtgcg tgacaaggat caatggccgt
     6121 cgttcagggg gcaacaggct gcgggggttg cggagggtca gggtttgccc gaacgatggg
     6181 acggcgagcg cggcgcgaac gttaaatgga agaccaaaat tcccggcctc gcacattcga
     6241 gtccggtcgt gtggggggat cgagtcttcg tcaccaccgc cgtcagcagt cgcggtcagg
     6301 acaacttccg gcatggcctg tacggcgacg gcacggcctc cgaagaccgc tcgctccatc
     6361 gctggaagct atacgcgctc gataagaaga gcgggaaggt tctctgggag agaactgctt
     6421 acgaaggcgt cccgcgcgag aaacgacaca tcaaggcgag ctatgccaac gccacgcccg
     6481 cgacaaatgg ccgctacgtc gtcgccttct tcggctcaca gggcttatat gctttcgaca
     6541 tgcaggggcg tctcgtctgg aagagagact tcggcgtgct gaacgcaggc gcgtatgatg
     6601 cgcctgatta cgaatggggc acggccagtt cgcccatcat ttataaagag atggtgatcg
     6661 ttcagtgcga cacgcagggc gagtcgtttc tgcacgccgc cagcctcaag accggcaaga
     6721 cgatctggaa gactccgcgc acggaacttc cctcgtgggg cacgccgacc atctatcccg
     6781 gcaagtcgcg ggtggaactg atcaccaacg cgtcgaattt catacgcggc tacgatccgg
     6841 agacgggcaa agaattgtgg cggctcggcg gcagttcaaa gatcaccgct ccgacgcctg
     6901 tgttttccgg cgacttgatc gtcgtggcga gcggtcgtcg ccccgaagcc ccgatcttcg
     6961 tcatacgcgc gggcgcaaac ggcgacatca cgctgcccgc cgggcaaagc tcgaatcaac
     7021 acatcgcctg gagcaagcag gcgcgcggct cttacatgcc gacgcccctc atctacggca
     7081 actatctcta cgtgctgtcg aatcagggag tcttcgattg ctacgatttg aagacgggag
     7141 cggagatcta ccgggagcgt attcagcatc gaggcggagg gttcagcgca tcgcccgtcg
     7201 ccgccgatgg gaagatctat ctaccgggtg aggacggaga catgttcgtc gtacgtgcgg
     7261 gcgcgaagtt cgagttgctg gcgacaaacc cgatgggcga gttgatgatg gcgactccgg
     7321 ccatctcttc cggcatgatg ttcgtgcgca cgcagcacca cctcttcgca ctcagccgca
     7381 caacccctta gcgacagctt gaagattcac ctgcgcgtcg aaaacagatg aggagagcgg
     7441 ccacgaaaac cgctctcctc attctatgtg cgcccgtgcc ggagactacg gcgcgggcaa
     7501 aggctgccaa ctgaacgcgc cgttgtaggc cgagttgctc gtcaggttga cttgtaaact
     7561 gccgtccgcc tccatcagat aaatatcctg ataaccgttc tggtagacga tgaaaccgat
     7621 ctgtgtgccg tcgggcgacc agcgcggctc gtaagagtcg tacgccgtag tgagttgcgc
     7681 atggttcagg ccgtccgcgc ctacgacgtg gacggtcgag gaactatcgg cgtaactgct
     7741 ataagcgagt ttcgtcccgt cgggcgacca atccggctcg aagttgaaag gcgggttagg
     7801 gagcacgttg cccgtgttgg acacgttggt ctggtcactt ccgtctgcgt ccatcacgta
     7861 gatttcggaa ccgttgaagc cccatctgac gaaagctatc cgggttccgt ccggcgacca
     7921 gacgggcgta tcgtctccgg cggtcgtagt gagccgcaca cggtctgtcc cgtcggcgtc
     7981 catgacgtag atgttcgggt cgccgtcgcg gctgctgacg taggcgatct tgctgccgtc
     8041 gggcgaccat gcaggcaggt aagaagtccc ggtcgggtct tgggaaagat tggtcaggcc
     8101 ggtgccgtcg gcgttgacga cgaagacatc ggagttatat tccgtgtcgt aactcgtgaa
     8161 ggcgagcttc gtgctgtcgg gcgaccacgt atgatagtaa gagtctaccg aggtggtgag
     8221 ccgtgtctgg ttcgagccgt ccgcgttcat caagtagatc tccgactggc cgtcgcggta
     8281 gctgatgaaa gcgatcttcg tgccgtcggg cgaccagcgc ggctcgtcgc tgtccaatgt
     8341 tttctccgtc aggctcgttt gcgcgctgcc gtcgccgtcc atcacgaata tctgcatggt
     8401 cactccgcga taactgccgt aggcgagttt gccgtcggcg gcgggcgtcg gagcgggaga
     8461 cggaagaccc ggctgccagt tgagaaggag cgcgccgggg caggccgacg cgtcgtacac
     8521 atccagagcg atgtagtaga cggtgtttgc cgtcgcgttg aaagagacga ggctgtggac
     8581 atcgaatggc acatcgtcgt tgctcaccac ctctgtgagt gcgccgaggc tgccgcccgt
     8641 gtagacgccg agcaccgtgt cagcatgccc gcacgtgttg cccgtgctgc cgtcggtgtc
     8701 gaaggttacg ttgccgcccg ctgcgggctg ccagcgatac cagacggaga atccggcggc
     8761 gtgctgcgcg tgatcaggtt caccgacctc cccggtcgcg taggtgttat tgacgctcac
     8821 gctccctgag ccgccgatga gttccagagc atccgcgaaa tcgtcattag atagtggcgg
     8881 agtgggcgtc ggcgtaggag tgggtgtcgg cgtcggggtg ggcgtcggcg tgggtgcgga
     8941 agagggcacg aagccccagc acgaagctga actgtccgac gactcaaagg tcagccgggt
     9001 aacgttgctg ccgtcggcat cgatcacgta gatgtcgaag atgtaagcag tgccggtgta
     9061 gctctggtag gaaagctgcg ttccgtcggg cgaccacgcg ggactatgat cgatggtcgc
     9121 atcgttcgac aggcgcgtga tgtcgctccc gtcggcgtcc atcaggaaaa tatcgctctg
     9181 tgcgccgatg tcgctgctga aagcgatctt tgtcccgtcg ggcgaccaca ccggcacgga
     9241 gttgttccgg ttgttgctcg tgaggctcgt ctggtcgctg ccgtccgagt ccatgacgaa
     9301 gatttccgat gagccgtcac ggtagctcct gaagacgatc tgcgtgccgt caggcgacca
     9361 ggcgggatcg aagtcgccgc tcgtttcggt ggtcaggcgc gtgcggttac tgccgtcggc
     9421 atccatgacg tagatgtcgt ggttgagtcc ggagccgtaa ctgacaaaag cgatcttcgc
     9481 gccgtttggc gaccacgcgg ggctgtaatc aaacgcggtg ttattggtca gccgggtttg
     9541 ttcgttgccg taggggttca tcacgtaaat ctcgccgatg ccgattccgc cgctcgcgtc
     9601 gcggtcgctg acgaaggcga tcttcgtgcc gtcgggcgac caggttggct ccgcatcgct
     9661 ggccgaattg ttggtgagtc gcgtctgctg gctgccgtca ggattcatga tgtaaatctc
     9721 ctgattgccg tcgcgctcgc tcgtgaatgc gatcttgctg tcgggcgtcg gagtcggcgt
     9781 cgggtcagga agtgtgagga gcgtggccga gtagttgttc catagattaa gcgcgggaaa
     9841 cgcggagggc gcggcagcga cgcttgcatg gcttgaggcg tgtttgacgt gcgccgagtg
     9901 cgggaggttc aggggataga ctgacgccgc gaaacagttc aacaggatcg ccagcgtgag
     9961 agttaagctg gtgagacgct ttacgagata tgaggtagac atcctttttt tctcctttgt
    10021 cgtgaggtta attgatgagt gtgccgcgtc gcttcgaggt ttcgactgcg cggcgcaagt
    10081 ttccttgatc gtaaagccac acgtcgttcg tcttgccggc gcacggagct ttgagccggc
    10141 gtgatgcggg tcaacatccg tgcttcgaca cacgcgcgtg cacgcgagat gtcagaacac
    10201 aattggtttt ctggcttgga acgcctcgga aacagtgagg tggcggcgtt cctgttcgac
    10261 tagcggggcg cgattctgac atgcgaaatg gcaggttgca ggctttctcg ccggcgaccg
    10321 acacttcgcc ccttctactt gagcaacaat aaacttcgtt cttggtttcg ggagaggaag
    10381 atgtatttga tgcttgaaaa ctcgttcgag ctttcgtcgt actggcgacg gtaacgttaa
    10441 ggattctagt ctcttaacga gaaatgtcaa gataaatgat taacgcgtac ggcttcccgg
    10501 cgggtcggcg tccgggtaag aaccgacgtg tgtgaacttc accagccgga gccgtccgtt
    10561 gcgccattcg tagtaataaa ttccgctcgc gccgtatcgt tcctcgttcg ccgcgccgat
    10621 gttcggacgc ccgaccacac gcagcaacct gctgttcgcg cgatacatcc acggctcttc
    10681 gccttcgggc ggttcataat cgccgacgat gcccgtccaa cccgtcaact cgttcgggaa
    10741 gaaggcacgc cccgtgcgcg catcaatgat cgagatgatc gaacaacccg tgccgcaacc
    10801 ggcggaggcg agcgtgtaat gacccgcgaa gttgacgccc tcttctttaa cctgttgccc
    10861 cagaagcgtc cggtacttgc gcgctaaagc atggctgcga agattcagcg gcatgacttt
    10921 tccccgccac acggcgaccg cgtaacgctc gaagcgcggc tcagtgctct tctcctgcgc
    10981 ccggctatga cccggcaaag acaggagcag gagcgcgaac acaaagagaa ttttcttacg
    11041 tctcaacata ctttttttca tctcaatctg atgcggaggc agcgtgtctc aggcggcgac
    11101 tttgcgcgct cagaccttca cccagtgaat atctaccacg gggctgttca cgtctttcaa
    11161 cgatctcagt tcaatcacaa acccgttctt caatctccac acatgcgaga cggagatgct
    11221 gttatccgaa gtctctttcg ccgcgccgtg cttttcggtc aacttcttta accatgtctc
    11281 gaacacctcc ttctgcttgt cataagcgta aactcccacg accacgtcta tcgcccccgc
    11341 ctccttttcg aagtagacgc gtgcgcgtcc ctcaaggctg ttgaacgtac ccgtccagac
    11401 gagttgcgtc tcgtcgtctt tggtcgcgcg gacgggacgc gcgccggtct tttcaatcgc
    11461 acgcttcacg tcctgaaaac ccatccccca tttgatctca ggaaagtctt tgaacaggtc
    11521 ggagggctga agcatcggct catcaccgcc gacctgtctg ccggacgact gtgacgactg
    11581 tatggcagac gcgtctgctg cttggaagac gcaggcaaaa catactataa acaccagacg
    11641 ccgcatgaat ttttgatagc ttcgcataca agtttctccc ctccataaac actctacacc
    11701 gaaagggcgg aggttttgaa gaagtttgaa cgtcgtgcga agcgtctttc atcagcggcg
    11761 tcgggaatag accggaagga gcctcgacat tcataagcct tcaaaggaag aactgctcgc
    11821 ggcggcgggg cggactgtgc cggacgtgat cgcgccggac ttgcgcgtgc tgttctgcgg
    11881 catcaacccc ggactctaca cggcggcgac cggctaccac ttcgcccgtc ccggcaaccg
    11941 cttctggcct gcgctctata cgagcggctt cacacgcagg ttgctctcgc ccgcagagga
    12001 gcacgaactt ctgacggacg gttacggcat caccaacgtc gtcgcccgcg cgactgcgac
    12061 tgccgccgag ttgtccgcag aagagtacgt cgagggcggt cggcggctca aagcgaaggt
    12121 gcgacgttat cgcccgaagt tcatcgcggt gcttggcgtc ggcgcatacc ggacggcgtt
    12181 cgaccgaccg aaggccacgc tc
//
LOCUS       DASWNS010000254         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_88202, whole genome shotgun sequence.
ACCESSION   DASWNS010000254 DASWNS010000000
VERSION     DASWNS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88202"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VGB76_08960"
     CDS             complement(<1..1347)
                     /locus_tag="VGB76_08960"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0099068.1"
                     /translation="MSVKTSALQYAEVAVPLRVAQTFTYRLPLALREEAQVGARLLVP
                     FGRKLLTAYIVALHAELEAGVELEESEIKEAEALLDAEPLISEDVLELTRWIADYYAA
                     PWGEVLKAALPAGLNSTVEQILTATAEGRDELARLPVRRAATHKARALQIIADESETT
                     LRSLATHFGQTRAQTLARQLDAHGWIKITHRARTAQARAKRRKAVRLLPLEQHAGEGA
                     RALNAAQERIVGALIEANGEMLFAELLDAAQASVSSIQTLERRGVVEVFVREVRRDPL
                     AHARLPEREEFTLTEEQAAAFGEIETALHAERAYAAFLLHGVTGSGKTEIYIRAMRTA
                     LKLGRAAMMLVPEIALTPVFSRRLRAHFGDAVAIFHSSLTTGERYDEWSRIRRGEARV
                     VIGTRSAIFAPVADLGIIIVDEEHEASYRQQESPYYHGRDTAVMRALKERAIVVLGS"
     gene            complement(1526..>2048)
                     /locus_tag="VGB76_08965"
     CDS             complement(1526..>2048)
                     /locus_tag="VGB76_08965"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0099069.1"
                     /translation="ELLQRAQLHPQFKDRIYIFGEGQPGARVAVVGESPGAPDIASGK
                     PFMGPAGDMLGRILSAIGLKRADCYLTNTVKMISSGDELTPDVLAFFTPYLHRELAVV
                     RPEVVVAFGNTPTRALLRTKKPISQMRGEFHVYQGSQLMPTFNPAYLLRDPTKKREVW
                     EDMKKVRALLAER"
BASE COUNT          330 a          613 c          723 g          382 t
ORIGIN      
        1 cgagccgagc acgacgatgg cgcgctcctt caaagcgcgc ataaccgccg tatcgcgccc
       61 gtgatagtaa ggcgactcct gctggcgata agaggcttcg tgttcttcgt ccacgatgat
      121 gatgccgagg tctgcgacgg gcgcgaagat cgccgagcgc gtgccgatta cgacgcgcgc
      181 ctcgccgcgc ctgatgcggc tccattcgtc gtaacgctcg cccgtcgtga gcgatgaatg
      241 aaagatcgcc acggcatcgc cgaagtgcgc gcgcaggcgg cgggagaaga cgggcgtgag
      301 ggcgatctca gggacgagca tcatggcggc gcgaccgagt ttgagcgcgg tgcgcatggc
      361 gcgaatgtag atttcggtct tgccgctgcc cgtgacgccg tgcaggagga aggccgcgta
      421 ggcgcgttcg gcatggagcg cagtttcgat ctcgccgaag gccgccgctt gttcctccgt
      481 cagcgtgaat tcctcccgct ccggcagacg cgcatgtgcc agcgggtcgc ggcgcacttc
      541 gcgcacaaag acttccacga cgccgcgccg ttcgagcgtc tgaatggacg agacggacgc
      601 ctgcgccgcg tcgagcaact ctgcaaacag catctctccg ttcgcttcga tgagcgcgcc
      661 gacgatgcgc tcctgcgcgg cgttgagcgc gcgcgcgcct tcgcccgcgt gctgttccag
      721 tgggagcagg cgcacggcct tgcgacgctt ggcgcgcgcc tgtgcggtgc gcgcgcgatg
      781 cgtgatcttg atccagccgt gcgcgtcgag ttggcgcgcg agggtctggg cgcgcgtctg
      841 tccgaagtgt gtggcgaggc tgcggagcgt cgtctcgctt tcgtcggcga tgatttgcag
      901 ggcgcgagct ttgtgcgtgg cggcgcggcg cacggggagg cgcgctagtt cgtcgcggcc
      961 ttcggcggtg gcggtcaaaa tctgttcgac ggtcgagttg agtccggcgg gcagcgcggc
     1021 tttgagcact tcgccccacg gcgcggcgta gtagtctgcg atccagcgcg tgagttccaa
     1081 cacgtcctcg gagatgagcg gttcggcgtc gagcaaagcc tccgcttctt tgatctccga
     1141 ttcttccaac tcgacgcccg cttccaactc cgcgtgcagc gcgacgatat aggccgtcaa
     1201 caacttgcgt ccgaaaggca cgagcaggcg cgcgccgact tgcgcctctt cgcgcagggc
     1261 gagcggcagg cggtatgtga acgtctgcgc gacgcgcagc ggcacggcca cctccgcgta
     1321 ttgcagcgca gaggtcttaa cgctcacgtc gggcgcgtta gtcgcatcgg gttcaggggt
     1381 gggaagtaaa actgtcgaac tttgcacggc gagttaaatt agcttgaagc gacgaaaagg
     1441 ggaagcggcg aaagtatgcc cggatgtcac gcccggactg ttgcggaaac tctcgatggc
     1501 ggcggtctgg atgcaaactg cgccctcaac gctccgcgag cagggcgcgc acctttttca
     1561 tgtcctccca gacttcgcgt ttcttcgtcg ggtcgcgcag caggtaggcg gggttgaagg
     1621 tgggcatgag ttggctgccc tgatagacgt gaaattcgcc gcgcatctgc gagatcggtt
     1681 tcttcgtgcg gagcagtgcg cgcgtcggcg tgttgccgaa ggcgacgacg acttcggggc
     1741 gcaccacggc gagttcacga tgcaggtaag gcgtgaagaa tgcgagcacg tcgggcgtca
     1801 actcgtcgcc gcttgagatc atcttgacgg tgttggtgag ataacagtcg gcgcgtttga
     1861 gtccgatggc ggagagaatg cgcccgagca tgtcgcccgc cggacccatg aacggtttgc
     1921 cgcttgcgat gtcgggcgcg ccgggcgact cgccgacgac ggcgacgcgt gcgcccggtt
     1981 gcccttcgcc gaagatgtag atgcggtctt tgaactgcgg gtgcagttgc gcgcgttgga
     2041 gaagctcg
//
LOCUS       DASWNS010000255         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_320483, whole genome shotgun sequence.
ACCESSION   DASWNS010000255 DASWNS010000000
VERSION     DASWNS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320483"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /locus_tag="VGB76_08970"
     CDS             <1..1846
                     /locus_tag="VGB76_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEY0099070.1"
                     /translation="AADYFTQTLAVWRNINDPVSESRALRKLGMIYSLQGSKKEALAN
                     LEQAITLTKTVAAPEFRAALLSHIGLVYNSLSDYRQALDYHQQALALNRSAGNVQGIS
                     SALTSIGSTYESQGDFQQALGFYRQAIDVRERMRSAARVEELQLGIAGQTANIYQYAI
                     KLCVQLNHTAQAFDLSESARARSLLDQLGNARISPLGTTDPQLLQQEQTLRAEISTLE
                     RQAMDERDTAQPGTETSPAKRQIQLNTLQAKYDELLTRLKVTNPEYVAMRTVAPLTLA
                     EIQRLLDKDTTLLSYFTTFDKTFAFIITRDSFHAVSLPVRESELRVAVQTFRSFASLD
                     DLHPQELRRLHGWLIKPIKPYLKTEAVSVIPHGVLNYLPFATLTDGQRYFGDEHRLSY
                     LPSASVLPFIQQKRKLSGGNLLALAQSSPPGLPVLQYADSMTAEIAKLYNSTPLHGSA
                     ATESVLHARASDSNILFLAAHANLNSISPLFSRIFLSPDKVNDGLLEVHEVYRLNLNN
                     TDLVVLSACQTSVGRHSSGDDIIGLNRAFIYAGTPTVVASLWSVKDLPTGELMTVFFK
                     NLRQGMSKAAALQAAQSKTREKYRHPYYWGAFVLTGDPGEKHARAAGR"
     gene            1857..>3503
                     /locus_tag="VGB76_08975"
     CDS             1857..>3503
                     /locus_tag="VGB76_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099071.1"
                     /translation="MLEPVIQFSPLSLSMFYRLTRSLEFIFRRERHPKEVFVMPLNLG
                     RFALLSLMAWLLCCFIISPAGSTHPVHRAQEPTSNVDPVLKVQGDAADKEAFALYLNG
                     DHQGALRRLADALALYRRARDRAGEANVLNGMGVIYASLNDFKKALKLYTEALSASEA
                     AGNSAHGALVATLSSLGALYESLGEYQKGLEVLQRGLGIATKNGDRKGEGAVRHHLGI
                     IHVRQGDYKQAVYHVEIALKIRRDEEQGDDFDVIATLNNLGLAYATTGRTQEGLARLG
                     EALNMAHVAGAPKDGVKVYNSIGRVRTGTGEYREAVKAYESARSVNARFVRDIRSEFE
                     SLQGLGLAYLSQQKLNDALNYYQQATVVASKTGNPFDEGTAFQRLGFVYERLTQFDKA
                     LQHYTRALRLHERLGQRAGEGETLISIGGIHRQRGQFAEALSYYDRALAIATGLNDLD
                     SKRIILNDTGEVYRSQGMYARALEFHQNSLRMSRETGNRIGEERSLGNIGGVYQSMGQ
                     PNTALGYYRQSLDIARSIGNRADEATNLNNIGNIALIYSSL"
BASE COUNT          793 a         1031 c          988 g          691 t
ORIGIN      
        1 ggctgcggat tatttcactc aaacactagc tgtctggcgc aacatcaatg accccgtcag
       61 cgaatcccgt gccctcagaa agctcggcat gatctacagc cttcaaggga gtaaaaaaga
      121 ggcgcttgcg aacttggagc aggcgattac attgactaaa accgtagccg ctccggaatt
      181 cagggccgcc ttgttgtccc atatcggctt agtctacaac tctctaagtg attaccggca
      241 ggcgcttgat tatcaccagc aggctcttgc gttgaatcgc tcggcgggga atgtgcaagg
      301 gatttccagt gcactcacga gcatcggaag cacgtatgag agccagggcg atttccaaca
      361 ggcgctcggt ttctaccgac aggccattga tgttcgggaa cgtatgaggt ctgccgcgcg
      421 tgtggaagaa ctccaattgg gtatcgcggg gcagacggcc aacatttatc aatacgccat
      481 caagctctgc gtgcaattga accacacggc acaagccttt gacttgagcg agagtgccag
      541 agcgcgcagc ctgcttgacc aactggggaa cgcacgcatt agcccgcttg gcaccaccga
      601 tccacagctt ctccaacaag aacagacatt gcgagccgag atcagcaccc tggaacggca
      661 agccatggac gagcgtgaca cagctcaacc cggtacggaa acctcaccag cgaaacggca
      721 gatacaactg aacacgctgc aagcgaaata cgacgaactg ctgacccggc tcaaagttac
      781 taaccctgag tatgtcgcga tgcgcacggt ggctccgctc acgttggcgg agattcagag
      841 gctgctggac aaagatacga cgctgctctc gtacttcacc acgttcgaca agaccttcgc
      901 ctttattatc acgcgcgact cattccacgc cgtttcgctc ccggttcgcg agagtgagtt
      961 gcgcgtggcc gtccaaacgt tccgcagctt cgccagcctc gacgaccttc acccacagga
     1021 actcaggcgg cttcacggct ggctcataaa gccaatcaag ccgtacctca agaccgaggc
     1081 tgtgagcgtc atcccccacg gggttctaaa ctatttgcct ttcgcaactc ttacggatgg
     1141 acagcgatac tttggcgatg agcaccggct ctcctacctg cccagcgcca gcgttctgcc
     1201 attcatacag cagaagcgta aattaagtgg gggcaacctg ctggctctcg cgcagagcag
     1261 ccctcccggc ctccctgtct tacagtacgc cgacagcatg actgcggaga tcgctaaact
     1321 ctacaactcc acgccgctgc atggcagtgc agccaccgag tcggttctgc acgcccgcgc
     1381 cagcgacagc aacattctct tcctcgccgc ccacgcaaat ctcaacagca ttagtccctt
     1441 gttctcgcgc atttttcttt cccccgacaa ggtcaacgac gggctgctgg aggtgcacga
     1501 ggtctatcgg ttgaatttaa acaataccga tctggtcgtg ttgagtgcct gccagacttc
     1561 cgtcgggagg cacagcagcg gtgacgacat aatcggactg aacagggctt tcatctatgc
     1621 ggggacgccg acggtagtag ccagcttatg gagtgtcaag gatctgccca cgggtgagtt
     1681 gatgacggtg ttcttcaaaa acttgagaca gggcatgagc aaggctgccg ctctacaggc
     1741 agcgcagtcg aagacccgcg agaagtatcg gcacccgtac tactggggtg catttgtctt
     1801 gaccggcgac cccggtgaga aacacgcacg cgcggcggga cgataaccgc cggtgcatgc
     1861 ttgagcctgt tattcaattt agcccgctgt ccttatccat gttctaccgg cttacccggt
     1921 cgctggaatt tatcttccgc cgggaaagac acccgaaaga ggttttcgtg atgcccctca
     1981 atctcggaag atttgcgctg ctatcgctga tggcctggtt gctctgctgc ttcatcataa
     2041 gccctgcagg gtcaactcat cccgtgcacc gcgcgcagga gccgacaagc aacgtcgatc
     2101 cggtgctcaa ggtccaaggc gacgcggcgg acaaagaagc cttcgccctc tacctgaatg
     2161 gcgatcatca gggagccttg cgccgactcg ccgatgcgct ggcactctac cgccgcgcgc
     2221 gcgaccgtgc cggggaagcg aacgttctca acgggatggg cgtaatttat gcatcgctga
     2281 acgatttcaa gaaagctctg aagctctaca cggaggcgct cagtgccagc gaagcggcgg
     2341 ggaacagtgc tcatggggcg ctcgtcgcca ctttaagcag cctcggcgcg ctttacgaga
     2401 gtctgggcga atatcagaaa ggtttggagg tcttacagag agggttaggc atcgccacga
     2461 agaatggtga tcgcaaaggc gagggggctg ttcgccacca ccttgggatc atccatgtcc
     2521 gtcagggtga ctacaagcag gctgtctatc atgtcgagat cgccttgaag attcggcggg
     2581 acgaggagca gggcgacgat tttgacgtta tcgccacgct caacaacctc ggcttggcat
     2641 acgccaccac aggccggacg caggaagggt tggcccgcct cggtgaggcg ctcaatatgg
     2701 cgcatgtcgc gggcgcgccc aaagacgggg tgaaggtcta caacagcatt ggccgcgtgc
     2761 gcactgggac gggtgagtat cgcgaagccg ttaaagccta tgagtcggca cgcagcgtta
     2821 acgctcgttt cgtgcgcgac atcaggagtg aatttgagtc acttcagggt ctgggtctgg
     2881 cctatctcag ccagcaaaaa cttaatgacg ccctgaatta ttatcagcaa gcaacggtcg
     2941 ttgcgtccaa gaccggcaac ccgttcgatg agggcacagc cttccaacgt ctcggcttcg
     3001 tctacgagcg gctgacgcag tttgacaaag ccttgcagca ttacacgcgc gctctgaggc
     3061 ttcacgagcg cctcggtcag cgcgctggcg aaggcgaaac gctcataagc atcggcggca
     3121 tccatcgcca acgcgggcag ttcgcggaag cgctgagcta ctacgaccgc gcgctcgcca
     3181 ttgcgaccgg actcaacgac cttgacagca agcggatcat tctgaacgac acaggcgagg
     3241 tctatcgcag tcagggtatg tatgcgcggg cactggagtt tcatcagaac tccctgcgaa
     3301 tgtcgcgcga gacgggaaac cgcatcggcg aggaaaggtc tctggggaac atcggcggcg
     3361 tttaccagag catgggtcaa ccgaacacag ctctcggcta ttatcggcag tcgctcgata
     3421 tcgcgcgctc aatcggcaac cgtgcggacg aagccacgaa cctgaataac atcggcaaca
     3481 ttgcgctgat ctactcaagc tta
//
LOCUS       DASWNS010000256         5297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_193345, whole genome shotgun sequence.
ACCESSION   DASWNS010000256 DASWNS010000000
VERSION     DASWNS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193345"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..534)
                     /locus_tag="VGB76_08980"
     CDS             complement(274..534)
                     /locus_tag="VGB76_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0099072.1"
                     /translation="MALKLQIQEIGRRQGIENAAQLAKLTGLGMTSAYQLWQGTAKAV
                     SLKTLNTLCNKLRTHPAILFDYKPDLEERNSITVKSRKGRNR"
     gene            729..1073
                     /locus_tag="VGB76_08985"
     CDS             729..1073
                     /locus_tag="VGB76_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099073.1"
                     /translation="MKKPSKIVSSKDDLLADDETWINILEPELCKDAAAIECLARVLF
                     TIKKEINSGSAGVKRANKILSNGIEVMYLYTNAHKAAIELYVLSLEGNLKPQDEPLNL
                     INAAIERGSSKR"
     gene            complement(1165..1716)
                     /locus_tag="VGB76_08990"
     CDS             complement(1165..1716)
                     /locus_tag="VGB76_08990"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0099074.1"
                     /translation="MKKLLLLACAAWLLVALFAYAERARVGAKLSGASLPFKAAMLYL
                     EPPDTTLAVPVEGVRAGQIKSSWQAPRSGGRLHEGQDIFAPRGTAVRSATAGYVVRVG
                     ENSLGGNTVFILGAGGRTYYYAHLDSYAPDATVGTYVTPQTVLGYVGTTGNATGTSPH
                     LHFGVYTSSGAIDPLPLLQDAPS"
     gene            1928..3574
                     /gene="treS"
                     /locus_tag="VGB76_08995"
     CDS             1928..3574
                     /gene="treS"
                     /locus_tag="VGB76_08995"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEY0099075.1"
                     /translation="MSGEAKLRNDPLWFKDAVFYEIYVRGFYDSNADGIGDFRGLTEK
                     LDYLEWLGIDCIWLLPMYASPLRDGGYDIADFYAMLPEYGTLEDFKTFLKEAHARGIR
                     VITDLVVNHTSDTHEWFREARRSPTSPKRDWYVWSDDPHKYAEARIIFTDTEHSNWTW
                     DEEAGAFYWHRFFSHQPDLNYDNPEVRQAMLDVVTFWLDLGIDGFRVDAVPYLIEREG
                     TNCENLPETHEVLKELRAHVEANYPNALLLGEANQWPEDVVEYFGGGTEFHMNYHFPI
                     MPRLFMALRQEDRRPIVEILERTPDIPENCQWGMFLRNHDELTLEMVTDEERDYLYNE
                     YARDKRMRLNVGIRRRLAPLMENSRRRIELLHSLLLSLPGSPFLYYGDEIGMGDNIYL
                     GDRDGVRTPMQWSADRNAGFSRADFERLYFPLISNPVYGYQSVNVESQQRYDSSLLNW
                     MRLMIHLRKRHRVFGRGRMEFVKPENRKIFAFTREHEGETVLCVFNLSQVAQPVELDL
                     AAYAGRTPVEMLGETPFPHITEQPYQLALAPFGFYWFLLR"
     gene            3632..>5297
                     /locus_tag="VGB76_09000"
     CDS             3632..>5297
                     /locus_tag="VGB76_09000"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HEY0099076.1"
                     /translation="MSTNENRSGRLVVVSNRLPFTLKRSGDGWRTERSTGGLATAMGP
                     ILKRTGGVWVGWPGDSSSPADERRQKTLGRWAERERYFAVDLPAEIAEGFYEGYSNQT
                     LWPLFHQFPSLLNFKPKHWQAYVEANRRFRDVVLEHLQPGDIIWVHDYQLMLLPQLLR
                     EAVPDARIGFFLHIPFPSSPIFRLLPRREELLQGLLGADYLAFHTYGYLQHFRASALR
                     ILGLDSRMDALEVGGRSVRLDALPIGIAPGEFTELLERDAETIARLDELRARFKGRRV
                     LLGVDRLDYTKGIPERLRTFRRLLKYAPELRGKVILIQVAVPSREVIPMYKELRHEVD
                     ELVGKINGEYSTPDWTPITYIRRGIPRSELAALYAVADLAWVTPLRDGLNLVAKEYVA
                     CQQEGAGVLVLSEFAGAASEMGEAFLVNPYDEERTAQIIERALALPAEVRRERISALH
                     KRVVRNNVFAWGDRFLANLREAAAASRAVNFSEKPQALPVEATVAAFQAAEHRRLLLD
                     YDGTLVPYVNRPQDAVPPAALVELLGRLAEDRSTAIAVVSGRSRHDL"
BASE COUNT         1107 a         1503 c         1542 g         1145 t
ORIGIN      
        1 gtcttaataa agaagattta cctacacctc ttttgccata gatagcaata aggccaagtg
       61 gagtattaat tgacttgata caatccctaa ctagatcagt tcggcctaca aatctattag
      121 gatcacgtat gacatctgat gtaaatccga tttctggatc aatcataatg agaatctttt
      181 aatagctaac ttacagttcg gaaaagtaaa aagtatgccg agacaattat attgtcaacg
      241 attttaaagg gagaaagtgt gtgaaaggta ggtctatctg ttccgtcctt tcctgctttt
      301 aaccgtaatt gaatttcttt cctccaagtc aggtttataa tcaaacaaaa tcgccggatg
      361 cgttctaagt ttattgcaaa gtgtattcag agttttcaat gaaaccgctt tagcagttcc
      421 ttgccaaagt tgataagcgg aagtcattcc caaacccgtc aactttgcta attgcgcggc
      481 gttctcaatg ccttgccttc tgcctatttc ttgaatttgt aatttaagtg ccatagccaa
      541 aactatagca tcggtatttt gttgatttta ggcgtgttac caagcttggg gagggggtat
      601 tttatccaaa ggaatggaga atatagtttc cctcaatttt gccccatcct ttattgataa
      661 aaaatcccat cgttggtaat atccccgtta gcagtatctc cgcctttagt aagattggtg
      721 aataactttt gaaaaaacct tctaaaatag tttctagtaa agatgattta ttagcagatg
      781 atgagacttg gattaatatt cttgaacctg aattatgcaa agatgctgcg gctatcgaat
      841 gtctagcacg tgtgctgttt actattaaga aggaaatcaa tagcgggtct gctggcgtga
      901 agagagcgaa taagatttta tcgaatggaa ttgaagttat gtatctctat actaacgcgc
      961 ataaggcagc tattgaatta tatgtcttat ctttagaagg taatttaaag ccacaggatg
     1021 aacctttgaa tttaattaat gcggcgattg agagaggtag tagcaaaaga tagtgatgaa
     1081 agggttgtta tcggcgcgtc cagtttagag cgcgccgcga gaaacgccga acggtttggt
     1141 cgctcgaagc ccggggatta ggagttaaga gggggcgtcc tgcaagagag ggagggggtc
     1201 gattgcgccg gaagaggtgt agacgccgaa gtggaggtga ggcgatgtgc cggtggcgtt
     1261 tcccgtggtg ccgacgtagc cgaggacggt ctggggggtg acgtaggtgc cgactgtcgc
     1321 gtcgggcgcg taagagtcga gatgcgcgta atagtatgtc cgcccgcccg cgccgaggat
     1381 gaagacggtg ttgccgccga gcgagttttc acccacccgc accacgtagc ctgcggtggc
     1441 tgagcggacg gcggttccgc gcggggcgaa aatatcctgc ccttcgtgga ggcgaccgcc
     1501 cgaacgaggc gcctgccatg aactcttaat ctgtccggcg cgcacgccct caaccgggac
     1561 ggcgagcgtt gtgtccggcg gctcaaggta aagcatggcg gctttaaacg gcaggctggc
     1621 gcctgacaat tttgcgccga cccgcgcgcg ctccgcgtag gcaaagagcg cgacgaggag
     1681 ccatgccgcg cacgccaata gcagcaactt tttcaccctg gttagatgtc gcaggcgggc
     1741 taatggttca ttgcgcgagc gcgtgccggg tcggcggggc ggccagttcg gcgcgcagtc
     1801 tcgcgccggg agtccgcccg ccggaagcgt gttgatcttg cgttgaatgc gagcgagcgg
     1861 ttatctttta ccccacaata caaatcaagt tttaaaaacc cggttcacag gaaggctgac
     1921 gaaagagatg tcgggtgaag cgaaactacg caatgatccg ctgtggttca aggacgccgt
     1981 cttttatgaa atctacgtgc gcggcttcta tgattccaac gccgacggca tcggtgactt
     2041 tcgcgggctg acggagaaac tcgattatct cgaatggctc ggcatcgatt gcatctggct
     2101 gctcccgatg tacgcctcgc ctctgcgcga cggcggctac gacatcgcag acttctacgc
     2161 gatgctgccc gaatacggca cgctcgaaga cttcaagacc ttcctcaaag aagcgcacgc
     2221 gcgcggcata cgcgtcatca cagacctcgt cgtgaatcac acttccgaca cccacgagtg
     2281 gtttcgagag gcgcggcgtt cgcccacatc gcccaagcgc gactggtatg tgtggagcga
     2341 cgacccgcac aagtatgcgg aggcgcgcat catcttcacc gacacggagc attcgaactg
     2401 gacttgggat gaagaggccg gggcgttcta ctggcatcgc ttcttcagcc accagcccga
     2461 tctgaattac gacaacccgg aagtccgtca ggccatgctc gacgtggtta ccttctggct
     2521 cgatctcgga atcgacggtt tccgcgttga cgccgtgccc tatctcatcg aacgcgaggg
     2581 gacgaactgc gagaacctgc cggagacgca tgaggtgctg aaagagttgc gcgcgcacgt
     2641 cgaggccaac tacccgaacg cgctcttgct cggcgaggcg aaccagtggc cggaggacgt
     2701 ggtggagtat ttcggcggcg ggacggagtt tcacatgaac tatcatttcc cgatcatgcc
     2761 gcgtctcttc atggccttgc ggcaggaaga ccgccgcccc atcgtagaga ttctggagcg
     2821 cacgccggac attccggaga actgccagtg gggaatgttc ctgcgcaatc acgacgaact
     2881 cactctggaa atggtgacgg acgaggagcg cgattatctc tacaacgaat acgcgaggga
     2941 caaacggatg cgtctgaacg tgggcatccg tcgccgcctc gcgccgctta tggagaacag
     3001 ccgccgccgc atcgagttgc tccacagtct gctcttgagc ctgccgggtt cgcccttcct
     3061 ctactacggc gacgagatcg gcatgggcga caacatctat ctcggcgacc gcgacggcgt
     3121 gcgcacgccg atgcaatggt ctgcggatcg caacgcggga ttttcacgcg ccgatttcga
     3181 gcgtctctat tttccgctca tcagcaatcc cgtctatggt tatcaatcgg tgaacgtcga
     3241 atcgcagcag cgttatgatt cgtcgctcct gaactggatg cgcctgatga tccatttgcg
     3301 aaagcggcat cgtgtgttcg ggcgcgggcg gatggagttc gtcaagccgg agaaccggaa
     3361 gattttcgcg ttcacgcgtg agcacgaggg cgagacggtg ctgtgcgtct tcaatctttc
     3421 gcaggtggcg cagcccgtcg aactcgacct cgccgcctac gccgggcgca cgcccgtcga
     3481 gatgctcggc gagacgccgt tcccgcacat caccgaacag ccgtatcaac tcgcgctcgc
     3541 gcccttcggc ttctactggt ttctgctccg gtaggacacg cggcgggcag cgaaatttcc
     3601 gactcttaat ttttttcgaa caaggggagc tatgtcaaca aacgaaaaca ggagcggtcg
     3661 tctcgtcgtc gtctcgaatc gcctgccgtt taccttgaag cgttcgggcg acgggtggcg
     3721 cacggagcgg agcacgggcg gactggcgac ggcgatgggt ccaatcctca aacgcacggg
     3781 gggcgtctgg gtgggctggc cgggcgattc ttcctctccc gcagacgagc ggcggcaaaa
     3841 gacgctcggg cgttgggctg agcgcgagcg ttacttcgcc gtcgatctcc ctgcggagat
     3901 cgcagagggt ttttacgaag gctattccaa ccagacgctc tggccgctct ttcaccagtt
     3961 cccctcgctc ttaaacttca agcccaagca ctggcaggct tacgtggaag ccaaccggcg
     4021 cttccgcgac gtggtgctcg aacatctcca acccggcgac atcatctggg tgcacgatta
     4081 ccagttgatg ctcctgccgc aactgctgcg cgaagccgtg ccggatgcgc ggatcggctt
     4141 cttcctgcac atcccttttc cctcttcgcc gatctttcgc ctgctcccgc gccgcgagga
     4201 actcctgcaa gggctgctcg gcgcagacta tctggccttt cacacctacg gctacctgca
     4261 acacttccgc gcctccgccc tgcgcatcct cggcctcgac agtcgcatgg acgcgctcga
     4321 agtcggcggt cgctccgtgc gtctcgatgc gctgcccatc gggattgcgc ccggcgagtt
     4381 tacggaactg cttgagcgcg acgcggagac aatcgcgcgg ctcgacgaac tgcgcgcccg
     4441 tttcaaagga cggcgcgtgc tgctcggcgt agaccgcctc gactatacga aaggcattcc
     4501 cgaacgcctg cgcacgttcc ggcgtctgct caagtatgcg ccggagctgc gcggcaaagt
     4561 gatcttgatt caggtggccg tgccctcgcg cgaagtgatc ccgatgtaca aggagctgcg
     4621 gcacgaggtg gacgaactcg tcggcaagat caacggcgaa tactcgacgc ccgactggac
     4681 gcccatcacc tacatccgtc gcggcatccc gcgctcagaa ctcgccgcgc tctacgccgt
     4741 cgcagacctc gcgtgggtca cgcctttgcg cgacgggctg aatctcgtcg ccaaagaata
     4801 cgtcgcctgc cagcaggagg gcgcgggcgt gctcgtcttg agcgagtttg cgggcgcggc
     4861 ctcggagatg ggcgaggcgt ttctcgtcaa cccttacgac gaggagcgca cggcgcagat
     4921 tatcgaacgc gcgctcgcgc tccccgcaga ggtgcggcgc gaacgcatca gcgcgctgca
     4981 caagcgcgtc gtgcgcaaca acgtcttcgc gtggggcgac cgtttcctcg ccaacctgcg
     5041 cgaggccgcc gcagcgtcgc gcgccgtcaa tttttcagag aagccgcagg cgttgccggt
     5101 cgaagcgacg gtcgcggcct ttcaagctgc cgaacatcgc cgcctcctgc tcgactacga
     5161 cggcacgctc gtgccttacg tcaaccgccc gcaggatgcc gtgccgcccg cagcgttggt
     5221 cgagcttctc ggcaggctcg ccgaagaccg ctcgacggcg attgcggtcg tctccggtcg
     5281 ctcacgccac gatctcg
//
LOCUS       DASWNS010000257        20367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_139421, whole genome shotgun sequence.
ACCESSION   DASWNS010000257 DASWNS010000000
VERSION     DASWNS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20367
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139421"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1029
                     /locus_tag="VGB76_09005"
     CDS             283..1029
                     /locus_tag="VGB76_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099077.1"
                     /translation="MFNTRKIFSTFAAFVLIAASALAFAPASVAAAGNADNKAKNVRR
                     VSAPLDLAILVQDDLVRRVGHELDVTREFVRELPSGSRVMVGYLTRGKLQVRQDFTTD
                     LEKAGRALRAPVGSELAAPFNPYVVVLEALRRFETDSKNRSAVLLISDGLDASRGLDP
                     SSTLNSIDLARAIDEAKRRHVAVYSFYAPTVGLTSFSRQAVSYGQGALNRFSNETGGR
                     AFFQGTDFVSFSPYFERLSRTLNSESGTGF"
     gene            1370..1717
                     /locus_tag="VGB76_09010"
     CDS             1370..1717
                     /locus_tag="VGB76_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099078.1"
                     /translation="MKTNSGREYCLIVEGPYLTEPEAEHALRDPFLEDWVEQTGRFRI
                     HNLKEIEVVPGVSLGMLGVRLLEEELFEIASLDPEHPLTEHKSKGVAEALKRYGMFDE
                     ISVEPREEIAGED"
     gene            1893..2477
                     /locus_tag="VGB76_09015"
     CDS             1893..2477
                     /locus_tag="VGB76_09015"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEY0099079.1"
                     /translation="MSDQTMNSFLNGRARVVVGDITRQATDAVVNAANSTLLGGGGVD
                     GAIHRAGGAEILAACREIRRTRYPKGLPTGEAVITTGGRLPARFVIHTVGPVWGQHAG
                     REAELLAACYRNSMALALEHKLVSISFPAISTGAFYYPPDKAAGVASGAIREFLTVGS
                     GGENEDARAKLEEVRLVFFSEADARVFLRHHKFE"
     gene            2589..4781
                     /gene="glgP"
                     /locus_tag="VGB76_09020"
     CDS             2589..4781
                     /gene="glgP"
                     /locus_tag="VGB76_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY0099080.1"
                     /translation="MEQVENKLGRALPETLSALEQLSLNYWWSWAADGAAVFRDLDPL
                     VWEESELNPRALLLDVSEYRLMQMATDPVYVERVRVLAEKFARYMNPQTKTWTRQSAP
                     GLTHENPVAYFCAEFGIHTSLPLYSGGLGMLAGDHLKSASDLGLPLVAVGLLYHQGYF
                     RQRLRRDGWQEEHYGTINPDKLPIQLLNDPATGKPLRIELEIRGRLVRFQAWQVLVGR
                     VKLCLLDSNLAENDHIDRLITAHLYGGDRETRCVQEMVLGIAGIRLLRKLGIEPHVFH
                     LNEGHSAFLTLELTRELVSQGKSFADAAREVRSRCVFTTHTPVAAGHDEFLPSLVEHC
                     FGRGYTESLTLTHEQFLDLGRVKAGSEDELFGLTPLALKMCRTTNGVSRMHGEVSRTL
                     WQKMYPHLEVKEVPITHVTNGVHAPTWVSPLLRRLYEEHVAPDWDENQCDAAFWQRGV
                     AQMPDEELWRVHRLMKQKLIAFIRTRQYFARLQRGESMEFVEAARRTFDPEALTIGFA
                     RRVAAYKRWSLILTDPDRLLKLINDAERPVQFVFAGKAHPQDQGAKQILQQLALWKLD
                     PQVINRTVFLQDYDQEIARKMVQGVDVWLNVPRRPLEASGTSGEKVAMNGGLNFSILD
                     GWWPEGYDTTNGWAIGDMHVEGENHEEDLRDAASLYDLLETRVVPAYYERDEHGIPRR
                     WVGMMKRAIETLVPAFNSDRMVQEYVQKIYTGIETGEAIESLHAPIGA"
     gene            4810..5019
                     /locus_tag="VGB76_09025"
     CDS             4810..5019
                     /locus_tag="VGB76_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099081.1"
                     /translation="MKEADGAHDSTKAGAQDGSTEEGGVGDVEAAGETTAAEMIGGAP
                     DTRPVGVDSIANIKISNQPEDDDKD"
     gene            complement(5151..5894)
                     /locus_tag="VGB76_09030"
     CDS             complement(5151..5894)
                     /locus_tag="VGB76_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0099082.1"
                     /translation="MNTNEIVSLSLLILLAAALYSSVGHGGASGYLAAMALFGLAPAQ
                     MKPTALCLNILVASIATFRFYRANCFSWPLFWPFAVASIPLAFVGGAITLPTAVYKQI
                     VGVTLLYAASRLFFYTRAKAEAQATRNAPLVPAILLGALIGFLSGLTGVGGGIFLSPV
                     LLLCGWADTRRTAGVSAAFILVNSIAGLLGNFSNVQMLPHALPYFALAAVAGGLAGAE
                     FGSRRLASVTIRRLLAVVLVVAGVKMIFV"
     gene            complement(5951..6814)
                     /locus_tag="VGB76_09035"
     CDS             complement(5951..6814)
                     /locus_tag="VGB76_09035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0099083.1"
                     /translation="MTILNRLGQRSTTFLITLGFLLVALQSVINYLAGPDFSIALFYL
                     LPVSLVAWLVGRRAGILVSLACAAGHFLTEHHLGHFHQRSSILYFNAISWLGGFLFVT
                     YFVSALRRSHDQERKLARTDDLTGMINRRSFFEASQQEINRARRNKHPFTVAYMDVDD
                     FKLINDRYGHSIGDMVLKSVSQTIKSNIREIDVVARLGGDEFVILMPETDEAAAQTVV
                     ARVHRNVMDKVHEYGWPITFSIGVVTWMTAPRTVDVMLKQADDAMYEVKNSGKNHVTH
                     LKLSGEPVAVA"
     gene            7062..9011
                     /locus_tag="VGB76_09040"
     CDS             7062..9011
                     /locus_tag="VGB76_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0099084.1"
                     /translation="MKTCPQCGSSYENAVTFCQRDGEVLEEAGVEMVGRVLDGQYEIE
                     SFIARGGMGRLYLARHILLGDRVAIKMLKPEMQSNAEWLRRFQREGRAARRFRHPNAV
                     TVYDLRTGADGVTYMVMEYVEGHTLDTELKRRGRLTPAEAWEILEPVSSALEAAHAQG
                     VVHRDLKPENVMINRTAAGELQVKLLDLGIAKLHDVAETHAGDGGAALTLAGQILGTP
                     YYMSPEQWGELPRDGNPEIDGRADIYSLGVICYELVRGEKPFTGYSLAELRQGHVSGH
                     RPLLHEVAEGVSPEFARAVERAMAKDRAERPATAGIFAAEILAALGGEHDSTQLALNS
                     GRGESAAAAVLPDLESSQTLIREKIAGTNAPRAQVQTVPQAASAQAATAYETQAIAQP
                     AKELTLAHAPAHAPHAAERSPFKLIAVGALVLLLVGGTLAGGWFALSRWGAGNESRDV
                     GLPAAGAATSEPPARFELISYWLEVFDDAEQVEGRRVGAKVLSLRSGQQFKFHFTPRE
                     RGYLYIVGPGAGNATKTFLTARPLGVLKTNLALADADFIFPYGEGEVLELDKNPGAEE
                     YLIIFSHTPLLSPAFLAESAGRELAPAELKELEDLRVRAKTAAPVADVKELKSGDGGA
                     AVAVSVSSASAPVEPFVFDIRIEHH"
     gene            9108..10184
                     /locus_tag="VGB76_09045"
     CDS             9108..10184
                     /locus_tag="VGB76_09045"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEY0099085.1"
                     /translation="MRYKLFLPKLLFAALSAICAPHVARACAQEAQGAQPAAQDEGIV
                     TRGAFLTTRKKSAGREGKTASGEAAAAVTIPAKRNDPSSTASNSPGKAKKGNGKSLAS
                     AKTKMSAPKNGNAPAREENAREKNSVEKNSDGANANVAYSEAASNPRPIGLGYTLFTL
                     GDNGEAVRTDPTRQFRTGEGIRIALETNTDGYLYIFHTENDGEPSMIFPDVRLDGGSN
                     LVKAHVPYEIPSSREATEAMRWFVFTDPPAAERLYIVLTRQPLANVPAGEALASYCLD
                     KAHSCPWRPAAATWTELKGAQEREQVAVSRVADQGRTQTADEREATTRGLGLGVGAPS
                     PSVIRMTASSNSKMLVTTIDLIHK"
     gene            10199..13057
                     /locus_tag="VGB76_09050"
     CDS             10199..13057
                     /locus_tag="VGB76_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEY0099086.1"
                     /translation="MKSRRLIVLVLLTCCVSGAPFSFVVSSSTARTAATGASPGAPQA
                     ATGTEALRQGRRLLKAGKADQALPLLESALRTFTAGNAVRGQAATHDALGDLYSRQGQ
                     HATALVHYQKAHEGFRQASSRAAIVKRVTGIGDEEFNTNLMLAKIGEAHYRAGNVAES
                     GAAYARIEVKKPETSAIGTAKKASRWGSALGNLGRTIRDGEISVSTGVGVASAASAVQ
                     QTFELYRQSILYATYELGLGRVAYHNNKLEVAKKHFTNTLSATGSNIPLIGNIGQMRR
                     FRVAANTSLGDVALKQSEFKDALKLYTDAAKGAQSDERPDLRWPAQRGIGKATLALAE
                     QQTDEKKKAQLHQQGFDAYLAALKTIEDIRQGSVRADEARTTFLATTRDVFDEASGAF
                     AAAALAVAKDKAAPLDGHALAHAAQAFRIVEQGRARSLLDLLSETGTTISEGIPPALL
                     QRKRENLERQQEIAQQITGVSLTGEPPQEGIDKLEEELRTLSVAYDSLENEIRTASPR
                     YGALTAAQPLTLAEVQQTVLDADTTLLEYSLGRERSYLWAVTKTGVTLHQLPARDKLD
                     TLAMALRDRIVPQSLRRALLDTGGQTRGLAEQRGLALANNATNANVASFAEASHALYQ
                     NTVAPAASSITKRRLLIVADGALNYVPFESLVTNANTAATGGDYSTLPYLVKTNEVVY
                     APSASVVAAIRRQAAGGANQNARGLLVVADPIFDASDPRAKSQQAGATPTGATLAVDS
                     AVADITGAADKAASGNFKLARLNGTRAEAEQIRKLALASGLSPDIWLDLDASEGKVRA
                     TDVSKYRIVHVATHGLLNTERPQFTGVVLSLVGNREGDGFLRTDEVFNLRLGSPLVML
                     SACETGLGRERRGEGVIGLTRAFMYAGAPTVGVSLWSVSDNSTADLMTDFYSRLLAKG
                     SSPAPPAAMRAAQLSMIAGKKYSAPFHWAPFVLVGDWR"
     gene            13288..13650
                     /gene="ytxJ"
                     /locus_tag="VGB76_09055"
     CDS             13288..13650
                     /gene="ytxJ"
                     /locus_tag="VGB76_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HEY0099087.1"
                     /translation="MRETSEQSTNFAPVTDKGELDELLARSHDAPVVLFKHSTTCPIS
                     ARAHRQMLQLSTEEAGRIALLIVQRARELSQRVAEETGIQHESPQAIILRNGQAVWSA
                     SHFDITAEAVAQAVQENK"
     gene            13760..14131
                     /locus_tag="VGB76_09060"
     CDS             13760..14131
                     /locus_tag="VGB76_09060"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY0099088.1"
                     /translation="MNEMMRRRGPAIEPQEIVQEGKAGLRIGWGDGRSCGYGAAALRR
                     ICPCAQCVNEMTGQRMLDPGSVPDDLTIESVQIVGRYALTFRWSDGHQTGIYSFRLLR
                     ELCEQNEEISGSNDKLSRNSP"
     gene            14170..14586
                     /locus_tag="VGB76_09065"
     CDS             14170..14586
                     /locus_tag="VGB76_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0099089.1"
                     /translation="MAEAKKILVAEDDEVTRQLMCMTLERHGFDVVTAEDGIQAYEFA
                     LIQRPDLIITDVSMPGADGAHFVRRVRDTSEIAATPIIVTTGFGTGSATFTLTVGADA
                     YEPKPVDPESLLATVRRLLSEGDATGDERGALTENF"
     gene            14628..14951
                     /locus_tag="VGB76_09070"
     CDS             14628..14951
                     /locus_tag="VGB76_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099090.1"
                     /translation="MQEAIGWISSLILVLTIGKQVFKQWKEGSSENVSKWLFIGQFAA
                     SLGFTIYSWLVGNWVFVVTNAIMLLNALAGLFIVLHHRRRERREGKAAGAGETSGGEL
                     KAERA"
     gene            14984..15250
                     /locus_tag="VGB76_09075"
     CDS             14984..15250
                     /locus_tag="VGB76_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099091.1"
                     /translation="MNEKDFFNEQQEAKRANYSCPHCRERNDYEVRWLKRTKKRELPR
                     NASEQDRARFQKSRDYMVRIDDVLDCKKCRRRFDIPNAQSVVFI"
     gene            15294..15521
                     /locus_tag="VGB76_09080"
     CDS             15294..15521
                     /locus_tag="VGB76_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099092.1"
                     /translation="MEELIKQVSQRSGISEDQARKAVDTVMNYMRDKLPASVSGTIDS
                     TLGGGANVAGNVADTAQNVLGNLGGMFGKKE"
     gene            complement(15650..17011)
                     /locus_tag="VGB76_09085"
     CDS             complement(15650..17011)
                     /locus_tag="VGB76_09085"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0099093.1"
                     /translation="MNTTPLILIAEDEDLMRVILSGLLEEAGYRVAAAPNAEEALERF
                     AAEDVAVTLTDIRMAGMDGLALLDRIKDIDGEALVIVMTAYSSVDSAIAALRKGAYDY
                     ITKPFVNEDLLQSIKNAIRQRELFRENRALRRELERRYSFSEIIGTSEVLQSVFRLVE
                     KVAATNTNILVEGESGTGKELIARAIHHHSPRAARPFVAINCGALPETLLESELFGHA
                     KGAFTGATANKPGLLRAAEGGTIFLDEIGEISPAMQVKLLRAVQEHEVLPVGTTAPVS
                     FDARIICATNRDLEKEVEAGRFREDLFYRLNVIEIHLPPLRERREDIPLLVRHFITRT
                     AHEQGEAEKAIQPEAMSALINYRWPGNVRELQNAVERAFTLSGERIDLDSLPPRVREA
                     AAGAGVALRDPDGLRPTLAEIERRHLLETLAAVNQDKARAANILGIDLSTLYRKLKRY
                     DEV"
     gene            complement(17028..17462)
                     /locus_tag="VGB76_09090"
     CDS             complement(17028..17462)
                     /locus_tag="VGB76_09090"
                     /inference="COORDINATES: protein motif:HMM:NF040273.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5957 family protein"
                     /protein_id="HEY0099094.1"
                     /translation="MNKDALKGAGLALVAAYLFGCLLVLFSARGAPELSQVVVTGFAG
                     LCFSFWFVIPIGMALGVLIPKLSAGRSVGAAATGGALLGVAAGFIGGILLSLFLDWFG
                     IGFASGKSRLLYFPFVMGIYCAPWTSAYACFRRDTDQALRQT"
     gene            complement(17459..17908)
                     /locus_tag="VGB76_09095"
     CDS             complement(17459..17908)
                     /locus_tag="VGB76_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0099095.1"
                     /translation="MPLIQLETYVAAPVEFCFDLNLNVQMHEAAGHGRAVAGVTQGQL
                     QLGDTVTWEAVHFGVRQRLTSKIVACERPRMFVDEMQRGAFARWRHTHRFTAFDGGTR
                     VFDEVDYASPLGLLGKIADALFLERYMTRFLIRHNAQFKRMAEATQR"
     gene            complement(17929..19716)
                     /locus_tag="VGB76_09100"
     CDS             complement(17929..19716)
                     /locus_tag="VGB76_09100"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099096.1"
                     /translation="MLLLLLLLALFLLVLLGFYVFVAAPRSRTHQTFAAFVLCLALWT
                     VKDIVLWEFSGHAASAVWWANASFIISLVLQYSLVVFAWLFPENRRVPYRRAAVLFAP
                     GAVFVPATLANLMWQDVSFAGQQFHIELRPLAYAFGLYIYFLFGYGFALLYRKFRRYR
                     GQLWGQQLGAILWALALTGALTTLVSIVLPLVNNYSLLPYGAVFVLPGVLVYAYAISN
                     FKLFSLQSALDQFRLFPIAYKVALSIALVAVLSFAVLQIPIVWWSFGAGTREAWRRYL
                     VFSIVSALVPNLLLVALVIRSISRPLRRLTEAAVEVSGGKYGTQVEIGSTNDEVGLLA
                     ASFNEMSRKMASDIEHLRTMGEQLIRTEKLAAAGTLAAGVAHEVNNPLASISSLIQIL
                     QTRAAPANATDAQSAETREMLRLISTQIARITRVLRDMMDFARTRPPARAPLDVRHAL
                     EASIRLASFDKDFKRLTLTTRYDESAPLVSADTDQLQQVFLNLLLNARDAMPEGGDIR
                     VSTSFRRDARELSIEIEDNGAGIPPEHRAHIFDPFFTTKPAGTGTGLGLAVCYGIVTA
                     HGGRIEVAAGDNGRGTRVSVSLPIMADAQ"
     gene            complement(19831..>20367)
                     /locus_tag="VGB76_09105"
     CDS             complement(19831..>20367)
                     /locus_tag="VGB76_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099097.1"
                     /translation="EEEELSLMRRPISTRDAFKRFGVLLGAFVPAALFYRMFPYNADT
                     NISSGEQLFALLLLLAMNVICCLTGRFMGSVIGRRIDPYERGSWHLMLAYSLCVGAFW
                     GIVTGAAGGSLVFIIGALFGAFVAVPFGIAGFMLFTPLHRLMARGGMIDARHFWPLAC
                     GVVLTITALILSPNLYPY"
BASE COUNT         4165 a         6093 c         6468 g         3641 t
ORIGIN      
        1 tcttctattc acgattgacg attcacgatt tacgcccttg attcatcctt catcgttcct
       61 ccttcatcgt tcctccttcg tcgttttccc acggcatcca tccggcctcg tcgtgcatct
      121 taattctgcc aaaatagttc gttagtagtt ggcgcaaaaa atcggagcgc gcagggtgag
      181 cagttttgac cgggcgcgtt ttcttgcgag ccaggggtgt catttgacag ttgagtgtcc
      241 tcggaagatt cgtctgaact tcgggggaca aggggaaaag agatgtttaa taccagaaaa
      301 atcttctcga cgtttgcggc tttcgtgttg atcgccgcga gtgcgctcgc gttcgctccg
      361 gcgtccgtgg cggctgccgg gaacgcagac aacaaagcaa agaatgtgcg ccgcgtcagc
      421 gcgccgctcg atctggcgat cctcgtgcag gacgacctcg tccggcgcgt cgggcatgaa
      481 ctggacgtga cgcgtgagtt cgtgcgcgaa ttgccgtcag gttcgcgcgt catggtcggc
      541 tatttgacgc gcggcaagtt gcaggtgcga caggatttca cgacggactt ggagaaggcc
      601 ggacgcgcgc tgcgcgcccc ggtgggaagc gagttggccg cgcccttcaa tccttacgtg
      661 gtcgtcctcg aagccctgcg ccgctttgag acggacagca agaatcgcag cgcggtgctg
      721 ctcatctccg acggcctcga cgcctcgcgc ggactcgatc ccagttccac tctgaacagc
      781 atcgacctcg cgcgtgcgat tgacgaagcg aaacgtcgcc acgtcgccgt ctattcgttc
      841 tacgcgccga cggtcggcct gacgagtttc agccgtcaag cggtcagtta tggacagggc
      901 gcgctcaatc gcttctccaa cgagacgggc ggacgagcct tctttcaagg cacggatttc
      961 gtcagtttca gtccatactt cgaacgtctg agccgcacgc tgaacagcga atccggcacc
     1021 ggtttctaac ttttcgacgc cgtgccagcc cgctagccgg acgcgacgcg cgatctcaag
     1081 cgggagtttt cagacgccgc cgggagcatc cagcttccgg cggcgtctct tcatagaact
     1141 gctgacagtc aactattagc tatgagccgg aagttctgtc ttagctgaaa actgacagtt
     1201 aacagctcac ggcttctctg taatttgacg ttcagttcta cgcgcgtttc cttcctcgct
     1261 ccggtaaaaa agttttgccg cgcgtcggcg gcagatgtta aagtgctccc accttgcgcc
     1321 cctgcgacag ttcctatgcg acgggcggcg cgcctcaagt tggcagacca tgaagactaa
     1381 cagtggccgc gaatactgcc tgatcgtaga gggtccgtac ctgacggaac cagaggccga
     1441 gcacgcgctc cgcgatccgt ttctggaaga ctgggtcgag cagaccggac gttttcgcat
     1501 acataacctg aaagagatag aggttgtgcc cggcgtctcg ctcgggatgc tcggcgtgcg
     1561 tctgctggaa gaagagttgt tcgagatcgc aagcctcgac cccgaacacc cgctcaccga
     1621 gcacaaatcg aagggcgtcg ccgaagcgct caaacgctac ggcatgttcg acgagatcag
     1681 cgtcgagcca cgcgaagaga ttgccggtga ggattaactc aaaggcaccc ctgtcccgcc
     1741 tctccgagag gaagttgaca ggctccttcc acgcttcctt cgcgcctgtc gtcttaactt
     1801 gaagcggcaa gcgggcgcat cccggttttg aaataaactc cgaaacccac ccgtcgcgaa
     1861 ctgattttca agcctgacgg tgctacactt agttgtctga tcaaacaatg aacagttttc
     1921 tgaacgggcg cgcgcgggtc gtcgtcggcg acatcacgcg ccaagcgacg gacgccgtgg
     1981 tgaacgctgc gaactcgacg ctgctcggcg gcggcggtgt ggacggcgcg attcaccgcg
     2041 cgggcggcgc ggagattctc gcagcgtgtc gcgagattcg ccgcacgcgt tacccgaaag
     2101 gattgccgac gggcgaggcc gtcatcacga cgggcgggcg gcttccggcg cgcttcgtga
     2161 ttcacacggt cggccccgtc tggggacagc atgcggggcg cgaggccgaa ctgctggcgg
     2221 cctgctatcg caactcgatg gccttagctc tggaacacaa actcgtctcc atatccttcc
     2281 cggccatctc gacgggcgct ttctattacc cgccggacaa ggctgcgggg gtggcctccg
     2341 gggcgattcg ggaattttta acggtcggga gcggcggcga gaacgaggac gcgcgcgcga
     2401 agctcgaaga agtccgcctc gtcttttttt cggaggcgga cgcacgggtt tttttacgtc
     2461 atcataaatt tgaataactg tcagtaggca gttgccggtt gccgggtcga atcttcatgg
     2521 acttacgcct gaagcacgag acaaacggaa actgattgca ggcaatctac ggacagcctt
     2581 tgaggttaat ggaacaagtg gaaaacaaac tggggcgtgc gctgcccgaa actttgagcg
     2641 cattggaaca gttgtctctg aactactggt ggagttgggc tgcggacggc gcggcggtct
     2701 tccgcgatct cgatccgttg gtgtgggaag agtccgagtt gaacccgcgc gcgctgctgc
     2761 tcgatgtctc cgagtatcgc ctgatgcaga tggcgaccga tccggtctat gtcgagcgcg
     2821 tgcgtgtgct cgcggagaag ttcgcgcgct acatgaaccc gcagacgaag acctggacgc
     2881 gtcagagcgc gccgggactc acgcacgaaa accctgtcgc ctatttctgc gccgagttcg
     2941 gcattcacac ctctctgccg ctctactccg gcggcctcgg catgctcgcg ggcgatcatc
     3001 tgaaatccgc cagcgacctc ggcctcccgc tcgtcgccgt cggcctgctc tatcatcaag
     3061 gctacttccg ccagcgtcta cgccgcgacg gctggcagga ggagcactac ggcacgatca
     3121 accccgataa gttgccgatt caactcctga acgacccggc gacgggcaag cctctacgta
     3181 tcgagttgga gatacgcggg cggctcgtgc gttttcaagc gtggcaggtc ttggtcgggc
     3241 gcgtgaaact gtgcctgctc gattcgaacc tcgcggagaa cgatcacatt gatcgtctca
     3301 tcacagcgca tctctacggc ggcgaccgcg agacgcgctg cgtgcaggag atggtcttag
     3361 gcatcgcggg catcaggctg ctgcgcaaac tcggcatcga accgcacgtc tttcatctca
     3421 acgaaggcca ctccgctttt ctcacgctcg aactcacacg cgaactcgtc tcgcagggta
     3481 agagtttcgc cgacgctgcg cgcgaagtgc gctcgcgttg cgtcttcacg acgcatacgc
     3541 ccgtcgccgc cggtcacgac gaatttttgc cgtcgctcgt cgaacactgt ttcgggcgcg
     3601 gctacaccga atcgctgacg ctcacacacg aacagtttct cgatctcggt cgcgtcaagg
     3661 cgggcagcga agatgagttg ttcggcctca cgccgctggc tttgaagatg tgccgcacga
     3721 cgaacggcgt gagccgcatg cacggcgaag tctcgcgcac gctgtggcag aagatgtacc
     3781 cgcacctcga agtgaaagaa gtgcccatca ctcacgtgac gaacggcgtc cacgcgccga
     3841 cgtgggtgtc gccgctcttg cgccgcctgt acgaagagca cgtcgcgccg gattgggatg
     3901 agaatcagtg cgacgcggcc ttctggcagc gcggcgtggc gcagatgccc gacgaagagt
     3961 tgtggcgcgt gcatcgtctg atgaagcaga aactgatcgc cttcattcgc acgcgccagt
     4021 actttgcgcg gctccagcgc ggcgagagta tggagtttgt cgaagccgcg cgcaggacgt
     4081 tcgacccgga ggcgttgacc atcggcttcg cgcgtcgtgt ggcggcctac aagcgttgga
     4141 gtttgatcct gaccgacccg gacaggctgc tgaagttgat caacgatgca gagcgtccgg
     4201 tgcagttcgt cttcgcgggc aaagcgcacc cgcaggatca gggcgcgaag cagattctgc
     4261 aacaactcgc gctctggaaa ctcgacccgc aagtcatcaa ccgcacggtc ttcttgcagg
     4321 actacgatca ggagatcgcg cgcaagatgg tgcagggcgt tgacgtgtgg ctgaacgtgc
     4381 cgcgccgccc gctcgaagcg tcgggcacga gcggcgagaa ggtggcgatg aacggcgggc
     4441 tgaatttttc catcctcgac ggctggtggc cggaaggcta cgacacgacg aacggctggg
     4501 ctatcggcga catgcacgtc gaaggcgaaa atcacgagga agacttacgc gatgccgcct
     4561 cgctctacga cctactcgaa acacgggtgg tgcccgccta ttacgaacgc gacgagcacg
     4621 gcataccgcg tcgctgggta ggaatgatga aacgcgccat cgagacgctc gtgcccgcct
     4681 tcaacagcga ccgcatggtg caggagtacg tgcagaaaat ctatacgggt atcgagacgg
     4741 gcgaagccat agagtcgctg catgccccga ttggcgcgtg aagcgcgtgg acgagaaagg
     4801 agacgggcaa tgaaagaggc ggacggcgcg cacgactcta ctaaggccgg ggcgcaagac
     4861 ggttcgacgg aagaaggcgg cgtcggcgac gtggaagccg caggggagac gaccgccgcc
     4921 gagatgatcg gcggcgcgcc cgacaccaga cccgtcggcg tcgattcgat tgccaatatc
     4981 aaaatctcca accagccgga agacgacgat aaagactaag agttaaagca ttcgccacag
     5041 aggcacagag acaagaaggg ggcacagaga agattcaact ttctctgtgc ccccctcgcg
     5101 tgtccctgtg tctctgcgat gagtctttaa ttctttccgc gactctggaa tcaaacgaag
     5161 atcatcttca cgcccgcgac gacgagcacg acggcgagca ggcgacgaat cgtgacgctc
     5221 gccagtctcc ggctgccgaa ctccgctccg gcgagtccgc ccgcaaccgc cgccagcgcg
     5281 aagtaaggca gcgcgtgcgg gagcatctgc acgttactga aatttcctaa cagcccggcg
     5341 attgagttga cgagaatgaa cgcggcggag acgcccgccg tgcgccgcgt atcagcccac
     5401 ccgcacagca acagcaccgg actcaaaaag attcccccac ccacgcccgt cagccctgac
     5461 aggaaaccga tgagcgcgcc cagtagaatc gccggcacga ggggcgcgtt tcgcgtcgct
     5521 tgcgcctccg ctttcgcccg cgtgtagaaa aagaggcggg aggccgcgta gagcagcgtg
     5581 actccgacga tctgtttata gacggcggtg ggcagcgtga tcgcgccgcc gacgaaggcg
     5641 agcggaatgg aagcgacggc gaaaggccag aagagcggcc acgagaaaca gttcgcgcga
     5701 tagaagcgaa aggtggcgat tgatgcgacg aggatgttga ggcagagcgc ggtcggcttc
     5761 atctgcgcgg gcgcgagtcc gaagagagcc atcgcggcga gatagcctga tgcgcccccg
     5821 tgtccgaccg acgaatagag cgcggcggcg agcaggataa gcagggagag cgagacgatc
     5881 tcgtttgtgt tcatcaggtc aggggaggat ggcggatgtg atcgggcgtt gaaacgttaa
     5941 gttgaaaagt ttaagcgacg gcgacgggtt cgccggagag cttgagatgc gtgacatgat
     6001 tcttgccgct gttcttgact tcgtacatcg cgtcgtcggc ctgtttgagc atcacatcca
     6061 cggtacgcgg cgcggtcatc cacgtcacca cgccgatgct gaaggtgatc ggccagccgt
     6121 actcgtgcac cttgtccatg acgttgcggt gtacgcgcgc caccaccgtc tgcgccgccg
     6181 cctcgtccgt ctccggcatg aggatcacaa actcgtcgcc gccgagtcgc gccaccacgt
     6241 caatctcgcg gatgttgctc ttgatcgttt gcgacacgct tttgagcacc atgtcgccga
     6301 tgctgtggcc gtagcggtcg ttgatcaact tgaaatcgtc cacatccatg taggcgacgg
     6361 tgaaggggtg cttgtttctg cgtgcgcggt tgatctcctg ctgcgaggct tcgaagaaag
     6421 agcgtcggtt gatcatgccc gtcagatcat ccgtgcgcgc gagcttccgt tcctgatcgt
     6481 gcgaacgccg gagggcggag acgaaatagg tgacgaagag aaaaccgccg agccacgaga
     6541 tagcgttgaa atagaggatg gaagagcgtt ggtggaaatg gccgagatga tgttcggtta
     6601 agaaatgtcc ggcggcgcag gcgagcgaga cgagaattcc ggcgcggcgt ccgacgagcc
     6661 atgctacgag cgagacgggc aacaggtaaa agagcgcgat ggaaaagtcg ggtccggcca
     6721 gataattgat aacgctttgc agggcgacga gaagaaaacc aagcgtgatg aggaatgtcg
     6781 tcgaacgttg tccgagccgg ttcaggatcg tcataaaaac tattctccga gcggtctggg
     6841 ggtaaacgct gagagcacgt tggcgaaacg tgccagagtt taaccttttg cttcgatgta
     6901 cgcatcaagt ttttggtttg tcgaaaactt aacatgaccc gcgcgccgct ccctagtcca
     6961 ttcacactcc cccgcgctca tgttagactt ttgtttatgt tcaacgctcc cgatagcagc
     7021 gtgagtctcc gcccgatcca ttctttcgta ctgtccctga catgaaaacg tgtccacaat
     7081 gcggcagcag ttatgagaac gcggtgacgt tttgccagcg cgatggtgaa gtgctggaag
     7141 aggcgggcgt ggagatggtc gggcgcgtgc tcgacgggca gtacgagatc gaatccttca
     7201 tcgcgcgcgg cggcatgggc aggctctacc ttgcgcgcca catcctcctc ggcgaccgcg
     7261 tggcgatcaa aatgctcaag ccggagatgc agagcaacgc cgaatggctg cgccgtttcc
     7321 aacgcgaagg ccgcgccgcg cgccgctttc gacacccgaa cgccgttacg gtttacgatc
     7381 tacgcacggg cgcggacggc gtcacctaca tggtgatgga atacgtggaa ggccatacgc
     7441 tcgatacgga gttgaagcgg cgcgggcggc tcacgccagc ggaggcgtgg gagattctcg
     7501 aacccgtgtc gagcgcgctc gaagccgcac acgcgcaggg cgtagtgcat cgcgatctca
     7561 aaccggagaa cgtgatgatc aaccgcacgg cggcgggcga actgcaagtt aaactgttag
     7621 acctcggcat cgccaaactg cacgacgtag cggagacgca cgcgggcgac ggcggcgcgg
     7681 cgcttacgct ggccgggcaa attctcggca cgccttatta catgtcgccc gaacaatggg
     7741 gcgaactccc gcgcgacggc aacccggaga tagacgggcg cgcagacatc tacagcctcg
     7801 gcgtgatctg ctacgaactc gtgaggggcg aaaaaccttt caccggctac tcgctcgcgg
     7861 agttgcggca gggacacgtg agcgggcaca ggccgcttct gcatgaagtc gcagagggcg
     7921 tgtcgccgga gtttgcgcgg gcggtcgaac gcgcgatggc gaaagaccgc gcggagcgtc
     7981 cggcgacggc agggattttc gccgccgaaa ttctcgccgc gctcggcggc gagcacgaca
     8041 gcacacaact ggctttgaac agcggcagag gcgagagcgc agccgccgca gtgctccccg
     8101 acctcgaatc ttcgcagacg ctcatacgcg agaagatcgc aggcaccaac gcgccgcgcg
     8161 cacaggttca gactgtcccg caggcggcgt cggcacaggc cgcaactgcc tacgagacac
     8221 aggccatcgc acaacccgcg aaggaattaa ccttagcgca cgcgcccgcc cacgcgccgc
     8281 atgcggctga acgctcgccg tttaagttga tcgcggtcgg cgcgctcgtg ctgttactcg
     8341 tcggcggcac gttggcgggc ggttggttcg ccttgagtcg ttggggggcg gggaacgaga
     8401 gcagagatgt cggcttgcct gcggcgggcg cggcgacaag cgagccgccc gcgcgtttcg
     8461 agttgatcag ctactggctc gaagtcttcg acgatgcgga gcaggtcgaa gggagacgcg
     8521 tgggcgcgaa agtcctgtcg ctgcgttcgg gacagcaatt caagtttcac ttcacgccgc
     8581 gcgaacgcgg ctatctctac atcgtcggac cgggggcggg caatgccaca aagacttttt
     8641 tgaccgcgcg cccgctcggc gttctgaaaa caaaccttgc gctggcggac gcggatttca
     8701 tcttccccta cggcgaaggc gaggtgctcg aactcgataa aaatcccggc gcggaagagt
     8761 acctgatcat cttttcgcac acgcctctgc tctcgccagc atttctcgca gagagcgcgg
     8821 ggcgagaact cgcgcccgcc gagttgaagg agttggaaga tttacgcgtg cgtgcgaaaa
     8881 ccgccgcgcc ggtcgcagac gtaaaggagt tgaagagcgg cgacggaggg gcggcggtag
     8941 ctgtctcggt gtcgtcggcg agcgcgcccg tcgaaccgtt cgtctttgat attcgcatcg
     9001 agcaccattg atccctcgca cggctcgcgc cgcgtgtgag acgcacgcgc ccgctcgcgc
     9061 ttgcccttcg catctctctt tgacagcact gaaggagaaa ccctgatatg cgttacaaac
     9121 ttttcctgcc aaaacttctc ttcgccgcgc tctcggccat ctgcgctccg cacgtcgctc
     9181 gcgcgtgcgc gcaggaggcg cagggagcgc aaccggcggc gcaggacgaa ggcatagtca
     9241 cgcgcggcgc atttctcacc actcgcaaaa agagtgcggg cagagagggc aagacggcga
     9301 gcggcgaagc ggcggcagcg gtcacgatac cggcgaaaag aaacgatccg tcgagcaccg
     9361 cgagcaactc gccgggcaag gcgaagaagg gcaacgggaa gtctctcgcc agcgcgaaga
     9421 cgaagatgag cgcgcctaaa aacgggaacg ctccggcgag agaagagaac gcgcgcgaga
     9481 agaactccgt tgagaaaaat tcggacggcg cgaacgcgaa tgtcgcttat agcgaggccg
     9541 cgtcgaatcc gcgcccgatt ggtctgggct acacgctgtt tacgctcggc gacaacggcg
     9601 aggccgtgcg caccgacccg acgcgccagt ttcgcacggg cgaaggcatc cgcatcgcgc
     9661 tcgaaaccaa cacggacggc tacctctaca tctttcacac cgagaacgac ggcgagcctt
     9721 cgatgatctt tcccgacgtg cgactcgacg gcggcagcaa tctcgtcaaa gcgcacgtcc
     9781 cttatgagat tccttcgagc cgtgaagcca cggaagcgat gcgctggttc gtcttcacag
     9841 acccgcccgc cgccgaacga ctctacatcg tgctcacgcg ccaaccgctc gccaatgtgc
     9901 cggcgggcga agcgctcgcc agttattgtc tcgacaaggc gcactcctgc ccgtggcgtc
     9961 ccgccgctgc aacttggacg gaactcaaag gagcgcagga gcgcgagcag gtggccgtca
    10021 gccgggttgc agatcaggga cgcacgcaga ccgccgacga gcgcgaagcg actacgcgcg
    10081 gcctcggttt gggcgtaggc gcgccgtctc cctccgtgat tcgcatgacc gcttcttcaa
    10141 actcaaaaat gctcgtcacg accattgatt tgattcacaa gtaattatgg agatcgttat
    10201 gaaatcccga agattgatcg tgttggtcct gcttacctgc tgcgtctccg gcgcgccgtt
    10261 cagtttcgtc gtctcttcct ccacggctcg caccgccgcg acgggcgcgt ctccgggcgc
    10321 accgcaggcg gcgacgggca cggaggcttt gcggcagggg cgcaggctgt tgaaggcggg
    10381 caaggccgat caggctctcc ctctgttgga atccgcgctc aggactttca ccgccggtaa
    10441 cgccgtgcgc gggcaggccg cgacgcacga cgcgctcggc gatctctatt cgcggcaggg
    10501 gcagcacgcc accgcgctcg tgcattacca gaaagcgcac gaggggtttc gacaggcgag
    10561 cagcagggcg gccatcgtca aacgcgtcac aggcatcggc gacgaagagt tcaacaccaa
    10621 cctgatgctc gccaagatag gcgaggcgca ttatcgcgcg ggcaacgttg cggaatcggg
    10681 cgcggcctac gcgcgtatag aagtgaagaa gcctgagacg agcgccatcg gcacggcgaa
    10741 aaaggcgagc cgctggggca gcgcgctcgg caatctcggt cgcaccatca gggacgggga
    10801 aatctccgtc tcgaccggcg tgggcgtcgc cagcgcagcc agcgcggtgc agcaaacttt
    10861 cgaactctat cgccagagca ttctctacgc gacgtatgaa ctcggcctcg gtcgcgtggc
    10921 ttatcacaac aacaaactcg aagtggcgaa gaagcatttc accaacacgc tctcggccac
    10981 cggcagcaac atcccgctca tcggcaacat cgggcagatg cgccgcttcc gcgtggcggc
    11041 caacacgagc ctcggcgatg tcgcgctcaa gcagagcgag ttcaaagatg cgctcaagtt
    11101 gtacacggac gcggccaaag gcgcacagtc cgacgaacgc ccagacctcc gctggcctgc
    11161 gcagcgcggc atcggcaaag cgactctggc gctcgccgaa cagcagacgg acgagaagaa
    11221 aaaagcgcaa ctgcaccagc aaggcttcga cgcttatctg gcggcgttga agaccatcga
    11281 ggacatccgg caggggtctg tgcgcgcaga cgaagcgcgc accacttttc tcgccacgac
    11341 ccgcgatgtc ttcgacgaag cctccggcgc gtttgccgca gccgctctgg ccgtggctaa
    11401 ggacaaggct gctccgctcg acggccacgc gctcgcacac gcggcgcagg cgtttcgcat
    11461 tgtcgaacag ggacgcgccc gctcccttct cgacctgctc agcgaaaccg gaacgaccat
    11521 cagcgaaggc atcccgcccg cgctcctcca gcgcaagcgc gagaacctcg aacgccagca
    11581 agagatcgcg cagcagatta cgggcgtgag tttgacgggc gaaccgccgc aggagggaat
    11641 tgataaactc gaagaggaac tgcgaacgct ctccgtcgcc tacgacagtc tggagaacga
    11701 gatcaggacg gccagcccgc gttacggtgc gctgaccgct gcgcagcctc tgacgctcgc
    11761 cgaagtgcag cagaccgttc ttgacgccga cacgacgctg ctcgaataca gtctcggtcg
    11821 cgagcgttcc tatctctggg cggtgacgaa aacgggcgtc acgctccacc agttacccgc
    11881 acgcgacaaa ctcgacacgc tggcgatggc cttgcgcgac cgcatcgtcc cgcagagttt
    11941 gcgccgcgcg ctcctcgaca cgggcggcca gacgcgcgga ctggccgagc agcgcggcct
    12001 tgcgctggcg aacaacgcga ccaacgccaa cgtggcctcc ttcgccgaag cctcccacgc
    12061 gctctatcaa aatacagtcg cgcccgccgc ctccagcatc acaaaaagac gcctgctcat
    12121 cgtggccgac ggcgcgctca actacgtccc gttcgaatcg ctcgtcacca acgccaacac
    12181 ggcggcgacg ggcggcgact attccacgct gccctatctc gtcaagacta acgaagtggt
    12241 ctatgcgccg tcagcctcgg ttgttgcggc gatcaggaga caggcggcgg gaggcgcaaa
    12301 ccagaatgcg cgcggcctgc tcgtcgtcgc cgatccgatc ttcgatgcca gcgacccgcg
    12361 cgcgaagagt cagcaggcgg gtgcgacgcc gacgggcgca acgctcgcgg ttgacagcgc
    12421 ggtggcagac atcacgggcg cagccgacaa ggccgcgagc ggcaacttca aactcgcgcg
    12481 tctgaacggc acgcgcgcgg aagcggaaca gatcaggaaa ctcgcgctcg cctccggcct
    12541 cagccccgac atctggctcg acctcgacgc cagcgaaggc aaagtcaggg cgacggacgt
    12601 gagcaagtat cgcatcgtgc acgttgccac acacggcctg ttgaacacgg aacgcccgca
    12661 gttcacgggc gtcgtcctct cgctcgtcgg caaccgcgaa ggcgacggct tcctgcgcac
    12721 cgacgaagtc ttcaacttaa gactcggctc accgctcgtc atgttgtcgg cctgcgagac
    12781 gggactcgga cgcgagcggc gcggcgaagg cgtcatcggg ttgacgcgcg ccttcatgta
    12841 cgcgggcgcg cccaccgtcg gcgtcagcct ctggtctgtc tcagacaatt cgacggctga
    12901 cttgatgacc gatttctaca gtcgcctgct cgcgaaaggc tcttcgcccg cgccgcccgc
    12961 cgccatgcgc gccgcgcaac tctccatgat cgcgggcaag aaatacagtg cgcccttcca
    13021 ctgggcgccc ttcgtcctcg tcggcgattg gcgttgaaac aagggatgag ggatgaggga
    13081 tgagggatga ataagaaaag cactcaacag agttgaatag ttctctgatt catccctcat
    13141 ccctcatccc tcatcccttg ctttgtcgcc ttgttgtacg tgtggcgcag gtgcgttaat
    13201 atcaggcgcg ttccccttgt gagcaactga ataaatttta tgtggaaaat ggtgcgccaa
    13261 tttctgtgtg gcggaggcaa ttttgcaatg cgagagactt ctgaacaatc gaccaacttc
    13321 gcgcccgtga cggataaagg agagcttgac gaattgctgg cgcgctcgca cgatgcgccc
    13381 gtcgttctct tcaaacacag cacgacctgc cccatcagcg cacgcgcgca ccgccagatg
    13441 ttgcaactct cgacggaaga ggccgggcgc attgcgctcc tcatcgtcca gcgcgcacgc
    13501 gaactctcgc agcgcgtggc ggaggagacg ggcatccagc acgagtcgcc gcaggccatt
    13561 atcctgcgca acggtcaggc cgtctggtcg gcctcccact tcgacatcac tgccgaagcc
    13621 gtcgcacagg ccgttcagga gaataaatag agtgacgagt gacgagtgac gagtgacagg
    13681 ttaaaacaga aaacctgttc ttaaccttct cttgtttcct gctcgtcact tgtcactcgt
    13741 cactcgtcac tgctttctta tgaatgagat gatgcgaagg cggggtccag ccattgagcc
    13801 gcaggagatt gtgcaggagg gcaaggcggg cttgcgcatc ggttggggcg acggacgttc
    13861 gtgcggctac ggcgcggcgg ctttgcggcg catctgcccc tgcgcgcagt gcgtcaacga
    13921 aatgaccggg cagcgcatgc tcgatcccgg cagcgtcccg gacgatctca ccatcgagag
    13981 cgtccagatc gtaggtcgct acgcgctcac cttccgttgg agcgacggcc atcaaacggg
    14041 catctacagc tttcgcctcc tgcgcgaact gtgtgaacag aacgaggaga tcagcggcag
    14101 caacgacaag ctctcaagaa attcaccttg atctgtcact tgtcgtctgc tgcttgtcat
    14161 tgttttatta tggcagaagc gaagaaaatt ctggtggcgg aagacgacga ggtcacgcgc
    14221 cagttgatgt gcatgacgtt ggagcgacac ggcttcgatg tcgtaaccgc agaggacggc
    14281 attcaggctt atgagttcgc tttgatccag cgtcccgacc tgattatcac agacgtttcc
    14341 atgcccggcg cagacggcgc gcatttcgtg cgacgcgtgc gcgacacgtc ggagatcgcg
    14401 gcgacgccca tcatcgtcac gaccggcttt ggcacgggca gcgcgacctt tacgctcacg
    14461 gtcggcgcag acgcttacga accgaagccg gttgatcccg agagcctgct cgcgacggtg
    14521 cggcggcttc tgtccgaggg cgacgcgacg ggcgatgagc gtggcgcgtt gacggaaaac
    14581 ttttagcagc cgttagcagg gcttattacc agggggcgaa gaagaggatg caggaagcaa
    14641 tcggatggat cagttcgttg atactcgtgc tgaccatcgg caagcaggtt ttcaaacagt
    14701 ggaaggaagg atcgagcgag aacgtttcga agtggctctt catcgggcaa ttcgccgcct
    14761 ccctcggttt cacgatctat agttggctcg tcggcaactg ggtcttcgtc gtcacgaatg
    14821 ccatcatgct cctgaacgcg ctcgccggac tcttcatcgt gctgcatcat cgccgccgcg
    14881 agcgtcgaga gggaaaggcg gcgggcgcgg gcgaaacgtc cggcggggaa ctcaaggccg
    14941 agcgcgcttg agttagtttc ccgatccgca gtatttttaa cttatgaacg aaaaagattt
    15001 tttcaacgag caacaggaag ccaagcgcgc taactacagt tgcccgcatt gccgcgagcg
    15061 caacgactac gaagtgcgtt ggctcaagcg gacgaagaag cgcgaattgc cgcgcaacgc
    15121 cagcgaacag gatcgcgcgc gtttccagaa gtcgcgcgat tacatggtgc gcatagacga
    15181 cgtgcttgac tgcaaaaagt gccgccgtcg cttcgacatc ccgaacgcgc agtccgtcgt
    15241 ctttatctag cgcgcacccg ttcgggcgcg aggccattgg aaggagaaaa tatatggaag
    15301 agttgatcaa acaggtgagc caacgctccg gcatcagcga agatcaggcg cgcaaggctg
    15361 tcgataccgt gatgaactac atgcgcgaca aactgcccgc ctccgtctcc ggcacgattg
    15421 acagcacgct cggcggcggc gcaaacgtcg cgggcaacgt cgccgacact gcacagaacg
    15481 tgcttggcaa cctcggcggc atgttcggga agaaagaata acgagtcgta ccgccaggac
    15541 tcagcgcagc gaacgccttc ctaagtccca agccccaagt ctcaactctc aggtctgaaa
    15601 gctaattaga cctgagagtt gagacttggg gcttgggact tacgcttgtt tacacttcgt
    15661 cgtagcgctt gagtttgcgg taaagcgtgg agaggtctat gccgaggatg ttggcggcgc
    15721 gggctttgtc ctgattgacg gcggcgagcg tttcgagcaa gtggcgtcgt tcgatctcgg
    15781 cgagggtggg gcgcaggccg tccgggtcgc gcagggcgac gcctgcgccc gccgccgctt
    15841 cacgcacgcg cggcgggagg ctgtcgagat caatgcgctc gccgctgagt gtgaaagcgc
    15901 gttcgacggc gttctgcaat tcgcggacgt tgcccggcca gcggtagttg atgagtgcgg
    15961 acatggcttc cggttggatg gctttttccg cctctccctg ttcgtgcgcc gtgcgcgtga
    16021 tgaaatgacg gacgagcaag gggatgtctt cgcgccgctc gcgcagaggg ggcaggtgaa
    16081 tctcgataac gttcagacgg tagaacaagt cctcgcggaa acggcctgcc tcgacctcct
    16141 tctccagatc gcggttcgtc gcacagatga tgcgcgcgtc gaacgagacg ggcgcggttg
    16201 tgcccacggg taagacttcg tgctcctgca cggcgcgcag aagtttgacc tgcatggcag
    16261 ggctgatctc gccgatctcg tcgagaaaga tcgtgccgcc ttccgccgcg cgcaggagtc
    16321 cgggtttgtt cgcggtcgcg ccagtgaaag cccctttcgc gtggccgaag agttcggatt
    16381 cgagcagcgt ctcggggagc gcgccgcagt tgatggcgac gaaggggcgc gcggcgcgcg
    16441 ggctgtggtg atggatggcg cgtgcgatca gttctttgcc cgtgcccgat tcgccttcga
    16501 cgagaatgtt ggtgttcgtc gccgccactt tttcgacgag acgaaagacg gactgcaaaa
    16561 cctccgacgt gccgatgatc tcggagaaac tgtagcggcg ttcgagttca cgccggagcg
    16621 cgcggttctc gcggaagagt tcgcgctgcc ggatggcgtt cttaatggac tgcaacaggt
    16681 cttcgttgac gaaaggcttc gtgatgtagt cgtacgcgcc tttacgcagc gcggcgatgg
    16741 cggaatcgac ggacgagtaa gcggtcatca cgatgacgag ggcttcgccg tcaatgtcct
    16801 tgatgcggtc gagcagtgcg aggccgtcca tgcccgccat gcggatgtcc gtgagcgtga
    16861 cggcgacatc ttcggcggcg aaacgttcca gagcctcttc ggcgttcggg gctgccgcga
    16921 cgcgatagcc agcctcttcg agcaggccgg aaaggatgac gcgcatcagg tcttcgtctt
    16981 cggcgatgag tatgagcgga gttgtgttca taatctgtat ggcgaattta cgtttggcgc
    17041 aacgcttgat ccgtgtcgcg tcgaaagcag gcataggcgc tcgtccaggg ggcgcagtaa
    17101 atacccatga cgaatgggaa gtaaagaagc cgactcttcc ccgaagcgaa gccgataccg
    17161 aaccaatcga gaaaaagaga aaggaggatg ccgccgataa aaccggctgc gactcctaac
    17221 aacgcccctc cggtcgctgc tgccccgacg ctgcgacctg ccgagagttt ggggatgagc
    17281 acgcccagcg ccatgcctat cgggatcacg aaccagaaag agaaacagag tccggcgaat
    17341 cccgtgacca ccacttgaga caactccggc gcaccgcgcg cagagaatag caccagcaga
    17401 cacccgaata gataggctgc gacgagagct aaccccgctc ctttgagggc atccttgttc
    17461 atcgttgcgt cgcctcggcc atgcgcttga actgcgcgtt gtggcggatg agaaaccgcg
    17521 tcatgtagcg ttcgagaaac aacgcgtcgg caattttgcc gagcagtccg agcggcgagg
    17581 cgtagtcaac ttcgtcaaac acgcgcgtgc cgccgtcgaa cgcagtgaac cggtgcgtgt
    17641 gccgccagcg agcgaacgcg ccccgctgca tctcgtccac gaacatacgc ggacgctcgc
    17701 aggctacgat ctttgaggtg agccgctgcc gcacgccgaa gtggacggct tcccatgtga
    17761 ccgtgtcgcc cagttgtaac tgaccttgcg tgacaccggc gacggcgcgc ccgtgccctg
    17821 ccgcttcatg catctgcacg tttaagttga gatcgaaaca gaattcgacc ggagcggcga
    17881 cgtaagtttc cagttgaatc aacggcacga ttctctcccc ctgatctctc actgcgcgtc
    17941 tgccattatt ggcagcgaca ccgagacgcg tgtgccgcga ccgttatcgc ccgccgcgac
    18001 ttcgatccga ccgccgtgcg ccgtgacgat gccgtagcag acggcgaggc cgagtcccgt
    18061 gccggttccg gcaggcttgg tggtaaagaa aggatcgaag atgtgtgcgc ggtgttcggg
    18121 cgggatgccc gcgccgttgt cctcgatctc gatggagagt tcgcgcgcgt cgcgcctgaa
    18181 agaagtgctg acgcggatgt cgccgccttc gggcatggcg tcgcgcgcgt tgagcagtag
    18241 attgagaaag acctgttgca gttggtctgt gtcggcggag acgagcgggg ctgactcgtc
    18301 gtagcgcgtg gtcagtgtga gtcgcttgaa gtccttgtcg aaagaggcga ggcggatgct
    18361 ggcttcgagc gcgtggcgca cgtcgagcgg ggcgcgcgcg ggcgggcgcg tgcgcgcgaa
    18421 gtccatcatg tcgcgcagga ctcgcgtgat gcgcgcgatc tgcgtggaga tgagacgcag
    18481 catctcgcgc gtctccgcgc tctgtgcgtc agtggcgttg gccggggcgg cgcgcgtctg
    18541 gagaatttga atgagcgagg agatggaggc gagcgggttg ttgacttcgt gtgcgacgcc
    18601 tgcggcgagc gttccggcgg cggcgagttt ttcggtgcgg atgagttgct cgcccatcgt
    18661 gcgtagatgt tcgatgtcgc tcgccatctt gcggctcatc tcgttgaagg aggcggcgag
    18721 cagtccgacc tcatcgttcg tgctcccgat ctcgacctgc gtgccgtact taccgccgga
    18781 cacctcgacc gccgcttccg tcagacgccg gagcgggcgc gagatgctgc gtatgacgag
    18841 cgcgacgagc agcaggttag gcacgagcgc gctgacgatg ctgaagacga gataacgccg
    18901 ccacgcctcg cgcgtgcccg cgccgaaact ccaccagaca atcggaattt gcagcacggc
    18961 gaaactgagc acggcgacga gcgcgatgga gagcgcgact ttgtaggcga tgggaaagag
    19021 ccggaattgg tcgagcgcgg attgcagcga gaagagtttg aaattcgaga tggcgtaggc
    19081 atagacgagc acgccgggca ggacgaagac cgcgccgtag ggcaggagcg agtagttgtt
    19141 gacgagcgga agcacgatgg agacgagggt agtgagcgcg cccgtcaagg cgagcgccca
    19201 gaggatcgcg ccgagttgct gtccccagag ctggccgcga tagcgtctga attttcggta
    19261 gagcagggca aagccgtagc cgaagaggaa gtagatgtag agtccgaagg cgtaggcgag
    19321 cgggcgcagt tcgatgtgaa attgttgacc ggcgaagctc acgtcctgcc acatcaaatt
    19381 ggcgagcgtg gcggggacga agaccgcgcc cggcgcaaag agcactgcgg cgcgcctgta
    19441 aggcacgcgg cgattttcgg ggaagagcca cgcaaagacg acgagcgagt attgcagcac
    19501 gagcgagatg atgaaactcg cgttcgccca ccacaccgcc gaggccgcgt gcccgctgaa
    19561 ctcccacagc acgatgtcct ttacagtcca gagcgcgagg cagagcacga aggcggcgaa
    19621 ggtctggtgc gtacggctgc gcggcgcggc gacaaagaca tagaagccga gcagcacgag
    19681 caggaacagt gcgagcagga gtaggagcag cagcatacgg ttggctgtcc gtcgtcggta
    19741 gtcagtcgtc cgttgcctgc ggctggcgtg cgagtcgcga aagattcgga ttgaacaacg
    19801 ggcaacggat gaagtgcaac ggacagtctg ttagtacggg taaaggttcg ggctgagaat
    19861 caaggccgtg atggttaaga ccacaccgca cgcgagcggc cagaagtggc gcgcatcaat
    19921 cataccaccg cgcgccatga gacggtgaag cggggtaaac agcatgaagc ccgcgatgcc
    19981 gaacggaacc gccacgaacg cgccgaacag cgcgccgatg atgaagacca ggctgccgcc
    20041 cgccgcaccc gtgacgatgc cccagaaggc tcccacgcag agcgaataag cgagcatcag
    20101 atgccacgag ccgcgctcat aagggtctat tctccgcccg atcaccgaac ccataaaccg
    20161 ccccgtcaaa caacagatca cgttcatcgc cagaaggagc aggagcgcga ataactgttc
    20221 gccgctcgaa atgttcgtgt ccgcgttgta agggaacatc cggtaaaaga gcgcggctgg
    20281 tacgaatgcg ccgagcagga cgccgaagcg tttgaacgcg tcgcgcgtcg agatgggacg
    20341 ccgcatcagg ctcaactcct cctcttc
//
LOCUS       DASWNS010000258         7936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_88298, whole genome shotgun sequence.
ACCESSION   DASWNS010000258 DASWNS010000000
VERSION     DASWNS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88298"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VGB76_09110"
     CDS             <1..344
                     /locus_tag="VGB76_09110"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099098.1"
                     /translation="DEVAERLQLSEDAAFGLDMAVREAVTNAVLHGNKQDETKPVEIV
                     FTEAGGELVVTVRDRGTGFDPSSVDDPTAAQNLMKTSGRGILFMRNFMDTVEWSPHPE
                     GGTVVRMTKKR"
     gene            464..811
                     /locus_tag="VGB76_09115"
     CDS             464..811
                     /locus_tag="VGB76_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0099099.1"
                     /translation="MAELNINERQAGDVTVLDMSGKITIGEGSVALRSAIRRLIEEGK
                     KKILLNLSQVSYVDSSGIGEFVASFTAIGREGGQLKLLNLTQKIQDLLAITKLLTVFD
                     TYDDESSALNSFK"
     gene            949..1425
                     /locus_tag="VGB76_09120"
     CDS             949..1425
                     /locus_tag="VGB76_09120"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY0099100.1"
                     /translation="MFREAFRRLLAVDDPPERTALAFSIGIFIAFSPFLGLHTIMATL
                     IAFLFRFNKIAIYTGTFVNNPFLTLVPIIAASYALGALLMGRPLSLPEEGWRLLQEPH
                     LLTASYWQQLFTHLWDIVLPFSIGGMVLSIVLSLAAYPVTLKLLRARRRNQERDEG"
     gene            1621..3465
                     /locus_tag="VGB76_09125"
     CDS             1621..3465
                     /locus_tag="VGB76_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099101.1"
                     /translation="MKKHLFVIAALLAFGQTAASAQSSLPATRTPAAQPATPTPQQQR
                     QPTRSGTLDLSDYGVQIAPETRLVVMMAALDAAGFDPTPQGRQPSEFRAQVRRDQANL
                     DADLRTRLRRFYELNKLKNPAATPAEQAARYVSLAYALGAAPTFDSPARTGDLFGGAL
                     DVLDFAPLLREFYTKSGIAERLPAYLAAHQREGDRLRPQTSEMLRAVLTYLHTRPATF
                     SLERVPTRNPSAKPKKDAPPVFITRERERRFVLVPDLLAVPGSINFRVIGEDYFAVVP
                     AGIDPASSEVRRAYLQYVIDPIILRFNREVALKRAEIRELINSTSATTGAETPDVFPV
                     IARSLVIAADARMNLNTRLRALAEQTSVRLQSAKEPERAQILKESQADRAALEDALTA
                     QLADAYERGAVLAFYFSEQLRDQEIAGFDFADFFADMISRVDVAREKLRPSEYAAARE
                     RVAQLRREATRVAANRTNSETREDERLAALNQSIAEINELLRQRKYTEAEPRLRALMS
                     EHAGEPRIFFALAQAASISASDAFDDATRDERLGRALANYRFAINSATSETDRPLISR
                     AYTAMGRILAFLERRDEAIKAFEAALSLGDVKDGAYRDAAAELKQLRP"
     gene            complement(3580..4758)
                     /locus_tag="VGB76_09130"
     CDS             complement(3580..4758)
                     /locus_tag="VGB76_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0099102.1"
                     /translation="MFRTKTLLSLALLLLTLCAANAPAQEAQEARRAQTEGAYAFAFS
                     GNHLGVSVESVTRENMGSFNLRGEPRGVAVREVAENGPAAKAGLQKNDVILRFDGEQV
                     SSAQKLQRLINESAPEHTARLSISRGGAEQELSVTLGRREAFTPRAFGELKLPDVQAF
                     GMRADELKRHAEKWQRDSEEWKKRAKDLDRNSQEMRRNLEEKFRAEGFGDNFKLVYGG
                     GGRRIGITTQELTGQLANYFGVSERGGLLITSVAENSPAAKAGLRAGDVVNEVDGTRL
                     RNAGELSRALSRRDEGDVTLTITRDRKTRTIKVTPDRTQNSPTLYTPGGFGPAIAPMA
                     LSAPRISVAPMQVKPARPAMPAPPAAPRLAPFAVPRSPAAPRIYPMPAIPARPGVWVL
                     "
     gene            4916..5464
                     /locus_tag="VGB76_09135"
     CDS             4916..5464
                     /locus_tag="VGB76_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0099103.1"
                     /translation="MMKILDIGCGIKKTPGSIGLDSNPKTHADVIHDLDSVPYPFPDN
                     EFDVVVGNQVIEHIDDVLAVVAELYRITKPGGLIRLDTPHYSDIASYTDPTHKHHLTT
                     ESFAYFTGKRPDFAFYSDVRLRPRLVRVTLLKLWRALGIELLINSCNRYPALRFIRRF
                     WETYLCYVIRGKTIYFEFEVIK"
     gene            5822..7846
                     /locus_tag="VGB76_09140"
     CDS             5822..7846
                     /locus_tag="VGB76_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099104.1"
                     /translation="MLHVRKFYFAALATLALSFAFSSSQVVFAQAEDDVVVNEFVVNP
                     TTGKEYIELLVTKPGGVNMQGWTISDVGARATASGATEGDVTLPAAAYLANVPQGTFV
                     VIVLTTPAANTNTLTEDTSTTDGNRKLVLVIGTTAGLTLSGTIDNAASDNIQVYAGTR
                     AAGTLIDQTLAGTNTSLIAGATWGDNSTATVTDNINGGTGIPSNAAVRFVPTADTLAA
                     YQDNDTGLRFVVDAASYGTPGAPNTGIPGETAFFPPLIISEFRTFGRTGTCDEFVEIY
                     NTSGSPYTVQSQSAGSVGFAVAQSNNNVLFTIPNNTIIPARGHYLAVNNGGACTGSFS
                     GSTYPAGSPAATATGDNTLNEDIPTNAGIALFNTSLQAGFTLANRLDAVGTSLESNPL
                     YVEGTGYPVFSAALSGNPGVNYSIFRNAQSGAPLDTNNNETDFQTADINGTNLCSTTL
                     NFRCQRLGAPGPENLSSPVARNAEIKASLVDTQCASIAVNPTLRSACRFERRTGAGSG
                     FGSSPTSTGTLSIRRRFTNNTGVSITRLRFRIVDITTFPVPLGTADLRAISSTQVTAT
                     CQSEGGTPHLCTDNASPTTTIEGTTLETDASGQSNGGAFNSTLSLATPLAAGASVNVQ
                     FLLGVEQNGAFRFFINIEALPAPSGAVPAATGSATKAQAKGKARAAKGSN"
BASE COUNT         1515 a         2676 c         2275 g         1470 t
ORIGIN      
        1 cggacgaagt ggccgagcgt ttgcaactga gcgaagacgc cgccttcggc ctcgacatgg
       61 ccgtgcgcga ggcggtcacc aatgccgtcc tgcacggcaa caaacaggac gagacgaagc
      121 ccgtcgagat cgtgttcacg gaggcaggcg gcgaactggt ggtgacggtg cgcgaccgtg
      181 ggacgggctt tgatccatct tcggttgacg acccgaccgc cgcccagaat ttgatgaaaa
      241 cttccgggcg cggcatcctt tttatgcgca acttcatgga cacggtcgag tggtcgcccc
      301 atcccgaagg cgggacggtc gtgcgcatga cgaagaagag ataagacttg tccgtcgtcc
      361 gtcgtcagtg gccggttgtt caatccaaac tttagcaggt tgtgtgatta tcactggcaa
      421 ccgaccaccg acaactgatt accttttaag gagactccct gtaatggctg aattaaacat
      481 caatgagcgt caggccggag acgtgaccgt cctcgacatg agcggcaaga ttaccatcgg
      541 cgaaggttcg gttgcgctcc gaagcgcgat ccgtcgcttg atcgaagaag gcaagaagaa
      601 gattctcttg aacctctcgc aggtgagcta cgtggattcg agcggcatcg gcgaattcgt
      661 cgccagcttc acggccatcg ggcgcgaagg cggccagttg aaactgctca acctgacgca
      721 gaagattcaa gacctgctcg ccatcaccaa gctgctcacc gtcttcgaca cctacgacga
      781 cgaatcgagc gcgctcaaca gctttaaata acagtgacga gtgaccagaa agaaaagtga
      841 tgagtgatgg gtgatgagtg atgagtgaga accagagact tgtctttcac tcatcactca
      901 ttactcatca ctcatcactt cttccccgtc acttgtcact gttctcttat gtttcgtgaa
      961 gcatttcgcc gtctgctcgc ggttgacgac ccgccggagc ggacggcgct cgctttttcc
     1021 atcggcatct tcatcgcctt ctcgcccttc ctcggactcc acaccatcat ggcgaccctc
     1081 atcgcctttc tgttccgctt caacaagatc gccatctaca cgggcacctt cgtcaacaac
     1141 ccgtttctca cgctcgtccc catcatcgcc gcctcctacg ccctcggcgc gctcctcatg
     1201 ggacgcccct tgagtctgcc cgaagaaggc tggcggctgc tgcaagagcc gcacctgctc
     1261 accgcctcct actggcaaca actcttcacg catctctggg acatcgtgct ccccttctcc
     1321 atcggcggca tggtgctctc catcgttttg tccctcgccg cctatcctgt taccttaaag
     1381 ctgctccgcg cacggcgcag gaatcaagaa agggatgaag gatgagggat gagggatgaa
     1441 tcagaacagt gctcaacgaa gttaagcatt cctttcatcc cttatccctc tgccctccgc
     1501 cctttcttcc gctggcatcc ggcgcgacgg gtgccgcatc tttaagtagt gtcgcccgtg
     1561 ttgtaatttg agagttcctt cccggcgtgg aaaattcata tacaagtagt cgttaatcgt
     1621 atgaagaagc atttattcgt tatcgccgcc ctgctcgctt tcggtcagac ggcagcctcg
     1681 gctcaaagca gtttgcccgc gacgaggacg cccgccgccc aaccggcaac gccgacgccg
     1741 cagcagcagc gacagcccac gcgctccggc acgctcgatc tgtcggatta cggcgtgcag
     1801 atcgcgccgg agacgcggct cgtcgtgatg atggccgcgc tcgacgcagc cggtttcgac
     1861 ccgaccccgc agggacgcca gccctccgag tttcgcgcgc aggtgcgacg cgatcaggcc
     1921 aatctcgatg cagacctgcg cacgcgcctg cgtcgcttct acgagttgaa caaactcaag
     1981 aatccggcag ccacgcccgc cgaacaggcc gcgcgctacg tctcgctcgc ttacgcgctc
     2041 ggagccgcgc ccaccttcga ctcgcccgcg cgcacaggcg atctcttcgg cggcgcgctc
     2101 gacgtgctcg actttgcgcc gctcctgcgc gagttctata cgaagtcggg catcgcggag
     2161 cgtctgcccg cctatctcgc ggcgcatcaa agagagggcg accgcctgcg cccgcaaact
     2221 tccgagatgc tgcgcgccgt gctcacctat ctgcacacgc gccccgcgac cttctcgctc
     2281 gaacgcgtcc ccacgaggaa cccttcggcg aagccgaaga aggacgcgcc ccccgtcttc
     2341 atcacgcgcg aacgcgaacg acgcttcgtg ctcgtgcccg acctgttggc cgttcccggc
     2401 tcgatcaact ttcgcgtcat cggcgaagac tatttcgccg tcgtccccgc agggattgat
     2461 ccggcctcgt ccgaagtgcg tcgcgcttac ctgcaatacg tcatcgatcc gatcatcctg
     2521 cgcttcaacc gcgaagtcgc tttaaagcgc gcggagattc gcgaactgat caactccacg
     2581 agcgcgacga cgggcgcaga gactcccgac gtgtttccgg tcatcgcgcg ctcgctcgtc
     2641 atcgcggctg acgcgcgtat gaacctgaac acgcgcctgc gcgcgctcgc cgaacagaca
     2701 agcgtgcgcc tgcaaagcgc gaaggagccg gagcgcgcac agatcctcaa agagtcgcag
     2761 gccgaccgcg ccgcgctcga agacgctttg accgcccaac tcgcagacgc ttacgaacgc
     2821 ggcgcggtgc tcgccttcta cttctccgag caactgcgcg atcaggagat cgccgggttc
     2881 gacttcgcag acttcttcgc agacatgatc tcgcgcgtgg acgtggcgcg cgaaaaactt
     2941 cgcccgtcgg agtatgcggc ggcgcgcgaa cgcgtcgcgc agttgcgccg cgaggccacg
     3001 cgcgtcgcgg ccaaccggac aaactccgag acgcgtgaag atgaacggct cgccgcgctc
     3061 aatcagagca tcgcggagat taacgaactg ctgcgccaga gaaaatatac ggaggccgag
     3121 ccgcgcctac gcgcgctgat gagcgagcac gcgggcgagc cgcgcatctt cttcgcgctc
     3181 gcgcaggcgg ccagcatctc tgcgagcgat gccttcgacg acgccacccg cgacgaacgc
     3241 ctcggtcgcg ccctcgccaa ctaccgtttc gccatcaact ccgccacgtc ggagacagac
     3301 cgcccgctca tctcccgcgc ctataccgct atggggcgca tcctcgcctt cctcgaacgc
     3361 agggacgaag cgatcaaagc cttcgaagcc gcgctcagtc tgggcgacgt gaaggacggc
     3421 gcgtaccgcg acgccgccgc cgagttgaag cagttgcgac cttaagagtc ttcgacaata
     3481 aattgacccg ctgacgaaaa cgacgaaaac aacgacagcc ccgttatctc taacggggct
     3541 gtcgtcgttc tcaacgcgaa cgtcctctga gagttgtctt taaagaaccc acacgccggg
     3601 gcgcgccgga atggcgggca tgggatagat gcgcggggct gcgggcgagc gcggcacggc
     3661 gaacggcgcg aggcgcggcg cagcgggagg cgcgggcatt gcaggccgcg caggcttcac
     3721 ctgcatgggc gccacgctga tgcgcggcgc gctcaaggcc atcggcgcaa tggcaggtcc
     3781 gaagccgccg ggcgtgtaga gcgtcggact attctgcgtg cgatccggcg tcactttgat
     3841 ggtgcgcgtt ttgcggtcgc gcgtgatggt gagcgtgacg tcgccttcgt ctctgcggct
     3901 gagcgcgcgt gagagttcgc ccgcgttcct gagacgcgtg ccgtcaactt cgttgacgac
     3961 atcgcccgcc ctgagtccgg ccttcgccgc cgggctgttc tccgccacgg aagtgatgag
     4021 caggccgccg cgttcggaga cgccgaaata gttcgccagt tgccccgtca actcttgcgt
     4081 ggtgatgccg atgcgacgac cgccgccgcc atagacgagc ttgaagttgt cgccgaagcc
     4141 ttccgcgcga aacttctctt cgaggtttct gcgcatctcc tgcgagttgc ggtctagatc
     4201 tttcgcgcgt ttcttccact cctccgagtc gcgctgccac ttttcggcgt ggcgtttgag
     4261 ttcgtccgcc ctcatgccga acgcctgcac gtccgggagt ttgagttcgc cgaacgcgcg
     4321 cggcgtgaaa gcctcgcgcc tgccgagcgt gacggacaac tcctgttccg cgccgcctct
     4381 gctgatggag agccgcgccg tgtgttcggg cgcagactcg ttgatgaggc gttggagttt
     4441 ctgcgcgctg gaaacctgct cgccgtcgaa gcgcaggatc acgtcgttct tttgcagccc
     4501 ggccttcgcc gccggaccat tctccgccac ttcgcggacg gccacgccgc gcggctcgcc
     4561 gcgcaggttg aagctgccca tgttttctcg cgtcacgctt tcgacggaga cgccgagatg
     4621 attgccgctg aaggcgaatg cgtatgcgcc ctcggtctgc gcgcggcgcg cctcctgcgc
     4681 ctcctgcgcc ggagcgttcg cggcgcacag tgtcagtaac agcagggcga gggatagaag
     4741 cgttttggtt ctgaacatga atacaatcct ccgagagaga tttctggcaa tttcacgacg
     4801 cgccgcgcgg atggttccga aaactttcgt gcccgtcggc cacgcgcttt atgctgtgtc
     4861 gcactaaact tttgcgcgac gagtcgcact tggggtaagt ttccctttga gggaaatgat
     4921 gaaaattctc gacatcggct gcggcatcaa gaagacgccc ggcagcatcg gcctcgacag
     4981 caacccgaag acgcacgccg acgtgattca cgacctcgat tcggtgccct acccgtttcc
     5041 cgataacgag ttcgacgtgg tggtcggcaa tcaggtgatc gaacacatcg acgacgtgct
     5101 cgccgtcgtc gccgaacttt atcgcatcac caaacccggc ggcctcatac gcctcgacac
     5161 cccgcactac tccgacatcg cttcctacac agacccgacg cacaagcatc acctgacgac
     5221 cgaatccttc gcctacttca cgggcaagcg tccggacttc gccttctata gcgacgtgcg
     5281 cctgcgcccg cgcctcgtcc gcgtcacgct gctcaaactc tggcgcgcgc tcggcatcga
     5341 acttctcatc aacagttgca accgttatcc cgcgctccgt ttcatacgcc gcttctggga
     5401 gacgtatctc tgctacgtga ttcgcggcaa gaccatctat ttcgagtttg aagttatcaa
     5461 gtgaacaccg cgcgctcgcc gtagccctgc gtccggtgag gtagatgtgg ggagcggggg
     5521 aaaagtttca aaatggcgag aggtcgcggg agcgtgcggc ggcatttgct gtgcgtgcga
     5581 gcgataagat gaatcgtgta acgtaaatgt agggacagcc aagttgaccg cgctcgacgg
     5641 tttagcttat tctcggtgca ccaataaaaa attcgtcccc tcgaatttta agcggcaaac
     5701 tgaatcagaa agcgttgctg tctgacgccc tactgctgtt aaatcgaaga cgcatcaagc
     5761 cgcaattttg cccacacgct actttccatc taaacagcct aacttccagg agaatctctg
     5821 aatgctacac gttcgcaaat tttattttgc agcccttgct acgctcgctc tgagttttgc
     5881 tttctcaagc tctcaagtcg tcttcgcgca agccgaagac gatgtggtgg tcaacgagtt
     5941 cgtcgtcaac ccgacgacgg gcaaggaata tatcgaactg ctggtgacga aacccggcgg
     6001 cgtcaacatg cagggctgga cgatctctga cgtgggcgcg cgtgcgaccg cgtcaggcgc
     6061 gactgagggc gacgtgacgc tgcccgccgc tgcttatctc gccaacgtcc cgcagggtac
     6121 tttcgtcgtg atcgtcttga cgactcctgc ggcgaacacc aacacgctga ccgaggacac
     6181 gagcacgacg gacggcaacc gcaagttagt gctggttatc ggcacgaccg ccggactcac
     6241 tctatccggt acgatagaca acgcggccag cgacaatatt caggtgtacg cgggcacgcg
     6301 cgccgccggg acgctcatcg atcagacgct cgccgggacg aacacctcgc tcatcgcggg
     6361 cgcgacgtgg ggcgacaaca gcacggcgac cgtaaccgac aacatcaatg gcgggacggg
     6421 catcccgtcg aacgccgccg tccgcttcgt ccccaccgcc gacacgctcg ccgcttatca
     6481 ggacaacgac accggcctgc gcttcgtcgt ggacgccgcc tcttacggca cgcccggcgc
     6541 gcccaacaca ggcatccccg gcgagacggc attcttcccg ccgctcatca tcagcgagtt
     6601 ccgcaccttc ggacgcaccg gcacctgcga cgagttcgta gagatttaca acacgtcggg
     6661 cagcccttac accgtgcagt cgcaaagcgc gggttcggtc ggcttcgccg tcgcgcagtc
     6721 gaacaacaac gtcctgttca ccatcccgaa caacacgatc atcccggcgc gcggccacta
     6781 tctcgccgtc aacaacggcg gcgcgtgcac cggctccttc agcggcagca cgtacccggc
     6841 tggcagcccg gcggcgaccg ccacgggcga caacaccttg aatgaggaca tcccgacgaa
     6901 cgccgggatc gctctcttca acacgtcgct tcaggccggc ttcacgctcg ccaaccggct
     6961 cgacgccgtc ggcacgagtt tggagtcgaa cccgctctac gtcgaaggca cgggctaccc
     7021 cgtgttctcg gctgccctga gcggcaatcc gggcgtcaac tacagcatct tccgcaacgc
     7081 ccagagcggc gcgccgctcg acacgaataa caacgagacg gacttccaga ccgccgacat
     7141 caacgggacg aatttgtgct ccacgacgct caacttccgt tgccagcggc tcggcgcgcc
     7201 cggccccgaa aacctctcgt cgcccgtcgc ccgcaatgcc gagatcaagg cgtcgctcgt
     7261 ggacacgcaa tgcgcgagca tcgccgtcaa cccgacgctg cggagcgcgt gccgcttcga
     7321 gcggcgcacg ggtgccgggt cgggcttcgg ctcgtctccc acttcgacgg gcacgctctc
     7381 gatccgccgc cgcttcacca acaacacggg cgtgagcatc acgcgcctgc gcttccgcat
     7441 cgtggacatc accactttcc ccgtgccgct cggcacggca gacctgcgcg cgatctcttc
     7501 gacgcaggtg acggcgacct gccagagcga gggcggcacg ccgcacctct gcaccgacaa
     7561 cgccagcccg acgaccacta tcgaaggcac gacgctcgaa acggatgcgt caggccaatc
     7621 gaacggcggc gcgttcaact ccacgctctc gctcgccact ccgctcgcgg cgggcgcaag
     7681 cgtcaacgtg cagttccttt tgggtgtcga gcagaacggc gcgttccgct tcttcatcaa
     7741 catcgaagcc ctgcccgcgc cgtccggcgc agtaccggcg gctaccgggt cggccactaa
     7801 agcgcaagcg aagggcaagg ctcgcgccgc caaaggcagc aactaaacag gggcggacgc
     7861 tctccgcttc cccttcaacc tcgccggaac gcccacctcg cggctctccg gcaaaactta
     7921 aacagccgcc gcgcat
//
LOCUS       DASWNS010000259         9544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_218331, whole genome shotgun sequence.
ACCESSION   DASWNS010000259 DASWNS010000000
VERSION     DASWNS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218331"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..989)
                     /gene="uvsE"
                     /locus_tag="VGB76_09145"
     CDS             complement(66..989)
                     /gene="uvsE"
                     /locus_tag="VGB76_09145"
                     /inference="COORDINATES: protein motif:HMM:NF002640.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HEY0099105.1"
                     /translation="MSDSLTPQLGLVCITTTDAVRYRALTRKRLLQFDPPTQKAMLRE
                     LYADNLVRLNRALDFCSERGLRLYRLTSGLFPFADDETGADVLEEFRAEARRTGERAC
                     ALGIRLVIHPDQFVVLSSDSDQVVANSIKILETHAHVLDLLAQPRTSWTVIEIHGGKG
                     DRRARLVEVIQTLPEAIRTRLALENDEYAYSAAQILEVCRAARVPMVFDAHHHVCREK
                     LQSYEDESVACFLAAARETWPVPEWQLVHISNGRTAFNDRHHSDFVTDMPSAYARAPW
                     IEVEAKQKELAIEKLRGEWLGKGMRELRQTA"
     gene            complement(1178..2161)
                     /locus_tag="VGB76_09150"
     CDS             complement(1178..2161)
                     /locus_tag="VGB76_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY0099106.1"
                     /translation="MKVASQMKLNRFARYVWIVVAVNLFVIVWGAYVRASFSGDGCGS
                     HWPLCNGEVLPTTGLVKTLIELTHRLTSGVALLLVVWLVWRARQLFPRGHAVRRGAFW
                     SLIFILVEALIGAALVKFELVAGNASIARAFVMSLHLVNTFLLLAVLSLTAWWAMGGE
                     ALSLRGRGKVGAVFALGLCGTLLIAVSGGVAALGDTLFPASSLAQGIRQDFSTAAHFL
                     IRLRVLHPLFAVGIGLYVVASSSYVNNFHNSGQRTKRLTSILTTLFLVQLGAGILNVS
                     LLAPVWLQLTHLLLADLFWIALVLNAASALAQTDATTETLEHMDMRTAMET"
     gene            complement(2250..2726)
                     /locus_tag="VGB76_09155"
     CDS             complement(2250..2726)
                     /locus_tag="VGB76_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099107.1"
                     /translation="MPSNIPNSKARSGTNSPLENRMSTQAGTHPRWMTWTLYAAGAYN
                     LAWGAFVGLWPHAFFRWAGAELPNYPQIWQCLGMVVGVYGVGYAIAARDPFRHWPIVL
                     VGLLGKFLGPIGFVQTASRGEWPWALGWLNVTNDLIWLVPFTLILRAAYRDARATK"
     gene            complement(2671..3582)
                     /locus_tag="VGB76_09160"
     CDS             complement(2671..3582)
                     /locus_tag="VGB76_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEY0099108.1"
                     /translation="MKIIVTGSSGLIGSALVASLTQRGDEVTRLVREKSGAARAGEAR
                     EVLWSPSRGELNGAELEGHDAAIHLAGDPIAEGRWTDEKKRRIRESRVQGTTLLAGTL
                     ARLSAPPRVLLTASAVGYYGDRGAELLTEASAPGEDFLSGVCREWEASAEAAKAAGIR
                     VVKLRFGVVLSGAGGALTKMLTPFKLGVGGKIGSGKQYMSWIALEDALGAILHALTHE
                     ELAGAVNVVAPRAVTNAEFTKTLGSVLGRPTLFFVPAFAARLAFGEMADAALLASQRA
                     EPTQLKATGYAFKYPELEGALRHELAA"
     gene            complement(3774..5321)
                     /locus_tag="VGB76_09165"
     CDS             complement(3774..5321)
                     /locus_tag="VGB76_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0099109.1"
                     /translation="MKILLYNPDNGVTRNFMPHLWMFLLQTLTPPGHEVLLVDGNAHA
                     MNEEELVAFIREKEIGLVGIGAMTRMVAKAYRMADAVRAAGVPVVMGGPHVTECPDEA
                     LGRDGGTRHADAVALGEADETWPRIVEDAARGELKDIYLPLDAEGKDKKPSLQPYPLI
                     PWETLDLDQFNLVPKIARPLLERMGGGWGTFRVIPIESGRGCPYGCEFCTVTGFFGDS
                     IRFRSNESVVDEVLRLKARARKDGGQVAVFFIDDNFAINVKRTKSLLRDMIAANAQVP
                     WVAQISANLLKDEELVDLIAESGGKWVFIGMESIDPANMADVNKNFSKPGEYGAVLQR
                     LAARNIYAITSFIFGMDNDTPGVAERTLREIHSWPPGLPVFGQLTPFPATPLYDRLAA
                     SGRLARPRHWMDFAPFEMAHAPLKMTIKEARNEVNQAWTTSYSPARNAEAMDFIKDKP
                     VEVRLSHLVARLFFRGIYFPQMSKWAWIKLIADNRATLFGLVREGTGKWRAARKRRRR
                     AQSEATT"
     gene            complement(5650..6675)
                     /locus_tag="VGB76_09170"
     CDS             complement(5650..6675)
                     /locus_tag="VGB76_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099110.1"
                     /translation="MFKFRQFAAALFLVAATLASSVAVQAAFREYLPLVGAELGRPFA
                     VKYLGAIESRPSPHRQLDLGVATATLAYTEETELMLSGRDKAGHAWSVGLGDACGSGE
                     AYTADLDRNGQRDFLLVLPTCGNGLAPTHHLIAVTFESDGRPARFEVEGYFDADRKGI
                     FDLRDIDRDGRAELIYMNFSDGYWITNLYTATGARWRRIEGRFGGRAYPLHTRFTNRP
                     NRRAVRPAPGRRPFAPDLSNDAPRLRGQLRSYRWANVSQSEDIELNFETGEGRQVKCQ
                     PVSWYGSFTVVVDSARERKIFTLSSREEWFRPSLESLVEHRYEAALYGQRDAEHCSPE
                     TLWVTIK"
     gene            6920..7786
                     /locus_tag="VGB76_09175"
     CDS             6920..7786
                     /locus_tag="VGB76_09175"
                     /inference="COORDINATES: protein motif:HMM:NF018387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HEY0099111.1"
                     /translation="MRFSALVTICSLCCLICLSQTACGSASTEARAPRAATGTAAQAN
                     APEAARKSEPVAPVVGSIDADDDGLPDRAELSSYGDRENFRRWFTALAEMQFQEISKQ
                     WNAEQRDCAGLVRFSWREALRTHDRAWMQRMGDAYDAIAPDVHRTRTLERGALGARLF
                     RTRPGAFQERDLTDGTFSDFADARTLKEFNTEFVSRDRRRAESGDLIFFHQPWAQQYP
                     YHVMIFLGAAREASEGAADWVVYHTGASPGDRGTIKKVRLAVLDEHPDRRWRPVQSNR
                     NFLGFYRLKILR"
     gene            7972..>9544
                     /locus_tag="VGB76_09180"
     CDS             7972..>9544
                     /locus_tag="VGB76_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEY0099112.1"
                     /translation="MNRRKLLTSILIYLAVAFVIFSFSFWRLRMLAIGTSMGEAANEI
                     EYKERDPNVPAKPFFSLTTNRTYGTSERSRLWLNYRDIDAIDFRVYRVNDPAKFFRAL
                     DNPHQFGEDEEGEFESFYRKRKPTFLERVRAFKNIFYLGFRDYFRAQLKHESRKRFNQ
                     RFRAPDELKEAEAQRTPLNVADYARVPLLNEDQLVRGWREKLPPLDNEYDRRSIPLGQ
                     DKPGVYLVEAVNGDLRAFCVTIVTDLTMIEKTSADGQLLLYAVNRQTGAPRAGVQVQV
                     LQGKDTLTTGTTDNQGILRTKVERKQPKSAEEAAEGEGDEGAEESEGASDEGGEASYL
                     IMATERDNFAISDLDSYIFGGYGGEGENLTSYIYTDRPVYRPAQKVYFKGILRTLGER
                     GYQTPGGTITVSVADTNETKIFEREMPLSSRGTFSGEVDIPEDAPLGTYNVTATTANG
                     TASGYFDVQEYKKPEFKVAVSVPQRFAPTGSQVKFNVSARYFFGSPVTRADVKYYIYR
                     KRYYHYFSNPEADPID"
BASE COUNT         1909 a         3032 c         2849 g         1754 t
ORIGIN      
        1 aagactcttt ttgaccgttt ccaaggcata caggccgatc tttcattcag acctgagact
       61 aagcctcaag ccgtctgtcg caactctctc attcccttgc ctaaccactc cccgcgcaac
      121 ttttcaatcg ccagctcttt ctgcttcgct tcaacctcga tccacggcgc gcgtgcgtaa
      181 gcggaaggca tgtcggtgac aaaatcgctg tggtgacggt cgttaaaggc tgtgcgcccg
      241 ttcgagatgt ggacgagttg ccattcgggc acaggccacg tctcgcgggc tgcggcgaga
      301 aaacaggcca cgctctcgtc ttcatagctc tgcaacttct cgcggcatac atgatgatgc
      361 gcgtcaaaga ccatcggcac gcgcgccgca cggcaaactt cgagaatctg cgccgcgctg
      421 taagcgtact cgtcgttctc cagcgcgagc cgcgtgcgta tggcttcggg cagcgtttga
      481 atcacctcga cgagtcgcgc gcggcggtcg cctttgccgc cgtgaatctc gatgaccgtc
      541 caactcgtgc gcggctgcgc gagcaggtct aggacgtgcg cgtgcgtctc taaaatcttg
      601 atgctgttgg caaccacctg atccgaatct gaactgagca cgacgaactg gtctggatga
      661 atcacgagac gaatccctag cgcgcacgca cgctcgcccg tccggcgcgc ctccgcgcga
      721 aactcttcga gcacgtccgc gcccgtctca tcgtccgcaa agggaaagag tcccgaagtg
      781 aggcggtaga gacgcaggcc gcgttcgctg caaaaatcga gcgcacgatt cagccgcaca
      841 aggttgtctg cgtacaactc gcgcagcatc gctttttgcg tgggcgggtc gaattgcagc
      901 agacgcttgc gcgtgagcgc gcgataacgc acggcgtccg tggtggtgat gcagaccaag
      961 ccgagttgcg gcgttaaact atcgctcaat gcggaaattt cctcccaccc atctctcgtt
     1021 ctcaagcttc acgcatttaa gccggaaact ttatttttaa agacacgaat gtgtggcttg
     1081 tctgctgcgc tcccaatctc aaatggcttc tcaaacgacc ttagccttcg taaccgcacg
     1141 gccagcatcg cgccgctggc gacgacgatt gagagactca agtttccatc gcggtgcgca
     1201 tgtccatgtg ttcgagcgtc tcggtggtcg catccgtctg cgcgagggcg gaggcggcgt
     1261 tgagcacgag cgcgatccag aagaggtctg cgagcaggag atgcgtgagt tgcagccaga
     1321 cgggggcgag cagcgagacg ttcaggattc ccgcgccgag ttgcaccaga aagagggtgg
     1381 tgagaatgct cgtgagtcgc ttcgtgcgtt gcccggagtt gtggaagttg ttgacgtaag
     1441 aggaggaggc gacgacatac aagccgatgc cgacggcgaa cagaggatgc agcacgcgca
     1501 ggcgaatcag gaaatgcgcc gccgtggaaa aatcctgcct gattccttgc gcgagcgaac
     1561 tggcgggaaa gagcgtatcg ccgagcgcgg ccacgccgcc gctcacagcg atcaggagcg
     1621 tgccgcagag gccgagcgcg aagaccgcgc ccaccttgcc ccgcccgcgc aggctcaagg
     1681 cttcgccgcc catcgcccac cacgccgtca gcgacaggac tgcgagcaac aggaacgtgt
     1741 tgacgaggtg gagcgacatc acgaacgcgc gcgcgatgga agcgttaccg gcgacgagtt
     1801 cgaactttac aagcgctgca ccgatcaagg cttctaccaa aataaagatg agcgaccaga
     1861 acgcgccgcg tcgcactgca tgcccgcgcg gaaacagttg acgcgcacgc cacaccagcc
     1921 agacgacgag aagcaaagcg acgccgctcg tcaggcggtg cgtcaattcg atcagggtct
     1981 tgacgagtcc cgtcgtcggc agcacttcgc cgttgcacag aggccaatgg ctcccgcagc
     2041 cgtcgccgga aaatgacgca cgcacgtatg cgccccacac gatgacaaag agattgaccg
     2101 cgaccacgat ccagacgtag cgtgcgaagc gattgagttt catttgcgaa gcgactttca
     2161 ttgcgaagcg acgttctcta cagttcaaaa tttgcgaagg catctacatg gccggataag
     2221 cgaaattata agtcatgaag aggctgcggt catttcgtcg cgcgtgcgtc tctgtaggcc
     2281 gcgcgcagga tgagtgtgaa aggcacgagc cagatgagat cgttggtcac gttgagccag
     2341 ccgagcgccc aaggccattc gccgcgcgaa gcggtttgga caaaccctat gggaccaagg
     2401 aactttccga gcaggccaac gagcacaatc ggccagtggc gaaagggatc acgcgcggca
     2461 atcgcgtagc caacgccgta gacgccgacg accatgccga ggcattgcca gatttgcggg
     2521 tagttcggca gttcagctcc agcccaacga aaaaaggcgt gcggccacaa cccgacgaac
     2581 gcgccccacg cgaggttgta cgcgcccgcc gcgtagagcg tccaggtcat ccaacgcggg
     2641 tgcgttccgg cctgagtgct catccggttc tcaagcggcg agttcgtgcc ggagcgcgcc
     2701 ttcgagttcg ggatatttga aggcatagcc ggtagctttg agttgcgtag gttcggcgcg
     2761 ctggctggcg agcagcgccg cgtcggccat ctcgccgaac gcgaggcgcg cggcgaaggc
     2821 gggcacgaag aatagcgtgg gacgaccgag cacgctgccg agcgttttcg taaactccgc
     2881 gttcgtcacc gcgcgcggcg cgacgacgtt gaccgccccc gccaactctt catgcgtgag
     2941 cgcgtgcaga atcgcgccca acgcatcctc cagcgcgatc caactcatgt attgcttgcc
     3001 gctcccgatc tttccgccga cgcccagttt gaaaggcgtc aacatcttcg tgagcgcgcc
     3061 gcccgcgccg ctcaagacga cgccgaaacg caacttcacg acgcgaatcc ccgcagcctt
     3121 cgccgcttcc gcagacgctt cccattcgcg gcacacgccc gacaaaaaat cttcccccgg
     3181 cgcgctcgct tccgttaaaa gttccgcgcc gcgatctccg tagtagccca cggcggaggc
     3241 cgtgagcagg acgcgcggcg gcgcggacag gcgcgcgagt gtcccggcca gcagggtcgt
     3301 tccctgcacg cggctctcgc ggatgcgacg cttcttctcg tccgtccaac gcccttccgc
     3361 aatcgggtcg cccgccagat gaatcgccgc gtcgtgtcct tcaagctccg ccccgttcaa
     3421 ctcgccgcga cttggcgacc ataaaacttc tctcgcttcc cctgcgcgcg ccgcgccgct
     3481 cttctcgcgc acgagacgcg tcacctcatc gccccgctgc gtgagcgacg ccaccaacgc
     3541 cgaaccgatc aacccgctcg aacccgtcac gatgatcttc atcaacgatg ctccttctcg
     3601 tctcccttgc gcgcacaact acggcgcgtg cagcccccgg acgggcagag aacaaaccgc
     3661 cttgagtccc tttgcgactt tgcgcctttg cgtgagttta atatctcgca aaggcgcaaa
     3721 gtggcaaagg agaatcgctc ccctgcttaa aggttatttg tactcgctct ctgttatgtc
     3781 gtggcttcgc tctgagcgcg gcggcgtcgc tttcgcgcgg cgcgccactt gccggtgccc
     3841 tcgcgcacga gtccgaacaa cgtcgcgcgg ttgtcggcga tcaactttat ccaagcccat
     3901 ttgctcatct gcgggaaata gatgccgcga aagaagaggc gcgcgaccag atggctgaga
     3961 cgcacttcga cgggtttgtc cttgatgaag tccatcgcct ctgcgttgcg cgcggggctg
     4021 taggaggtgg tccatgcctg gttgacttcg ttgcgcgcct ctttgatcgt catcttgagg
     4081 ggagcgtgcg ccatctcgaa gggcgcgaag tccatccagt ggcgcgggcg cgcgagccgc
     4141 ccggaagcgg cgagacgatc atacaggggt gtcgcgggaa acggcgtgag ttgaccgaag
     4201 acgggcagcc ccggcggcca gctatgaatc tcgcggagcg tgcgctcggc cacgcccggc
     4261 gtgtcgttgt ccatgccgaa gatgaaagag gtgatggcgt agatgttgcg cgcggcgaga
     4321 cgctgcaaga ccgcgccgta ctcgcccggc ttgctgaaat tcttgttcac gtcggccatg
     4381 ttcgccgggt cgattgattc cattccgatg aagacccact tgccgcccga ttccgcgatc
     4441 aggtcaacga gttcttcgtc cttcaaaaga ttcgcgctga tctgcgcgac ccagggcacc
     4501 tgcgcgttgg ccgcgatcat gtcgcgcaaa agcgacttcg tgcgtttgac gttgatggcg
     4561 aagttgtcgt cgatgaagaa gactgcgacc tgtccgccgt ccttgcgcgc gcgcgctttg
     4621 aggcgcagca cttcgtcaac caccgactcg ttcgagcgaa aacggatcga gtcgccgaag
     4681 aatcccgtga cggtgcagaa ctcgcagccg taaggacagc cgcgccccga ttcaatcggg
     4741 atcacgcgaa acgtacccca gccgccgccc atgcgttcga gcagggggcg cgcgatcttc
     4801 ggcacgaggt tgaattgatc gaggtcgagc gtctcccagg ggatgagcgg gtagggttgc
     4861 aggctcggct tcttgtcttt gccttccgcg tcgaggggca gatagatgtc tttgagttcg
     4921 ccgcgcgctg catcttccac gatgcgcggc cacgtctcgt cggcttcgcc gagcgcgacg
     4981 gcgtcggcgt ggcgcgtgcc gccgtcgcga ccgagagctt cgtcggggca ttcggtgacg
     5041 tgtgggccgc ccatcacgac cggcactccg gcggcgcgca cggcgtctgc catgcgatag
     5101 gctttcgcca ccatccgcgt catcgcgcca atgccgacca gaccgatctc tttttcgcgt
     5161 atgaaggcga cgagttcttc ttcgttcatc gcgtgcgcgt tgccgtcaac cagcaacact
     5221 tcgtggccgg gcggcgtgag cgtttgcagc aaaaacatcc agagatgcgg catgaaattg
     5281 cgtgtcacgc cgttgtcggg gttatataac agaattttca agaatttact ttcccttcga
     5341 tagagcagga ctttgcgttt aaccttgagt tgaaataatg gccggagtaa acagcagacg
     5401 gcacagtaag ggggtttgaa gactccgcct tatgtcgagc agtgaacttc ggcggaagta
     5461 tagcagaatg tgtgcgccag cacacaaaga acttccggcc tgcccctgac gaccctcatt
     5521 tttacgtctg ccccgggaga aacggagaga gagtagcaca caaactccgg ggcgcgttct
     5581 tttgcgcagc gaaaggcagc ggcaaactct tccagccgcc ctccgtggcg accgggcggg
     5641 cggctctcat cattttatcg ttacccagag cgtctccggg ctacaatgtt ccgcgtcgcg
     5701 ttgcccgtag agagccgcct cgtagcgatg ctccacgaga ctttccagcg atggccgaaa
     5761 ccactcttcc cggcttgaga gggtgaatat tttccgctcc cgcgcgctat ccacgacgac
     5821 cgtgaaagac ccgtaccagg agacgggctg acacttcacc tgccgcccct cgcccgtctc
     5881 gaagttcaac tcgatgtcct ccgactgtga gacgttcgcc cagcgatacg agcgcagttg
     5941 accgcgcagg cgtggcgcgt cgttagagag atcaggcgca aacgggcggc gaccgggcgc
     6001 aggccgcacg gcgcgacgat tcgggcgatt cgtaaagcgc gtgtggagcg gataggcgcg
     6061 cccgccgaag cgtccctcga ttctgcgcca gcgcgcgccc gtcgccgtgt agaggttcgt
     6121 gatccagtag ccgtcgctga aattcatgta gatgagttcg gcgcgcccgt cgcggtcgat
     6181 gtcgcgcagg tcgaagatgc ctttgcggtc ggcgtcgaaa tagccttcga cttcaaagcg
     6241 cgcggggcga ccgtcgctct caaacgttac cgcgatgagg tgatgcgtgg gagcgaggcc
     6301 gttgccgcac gtggggagga cgagcaggaa gtctctctgg ccgttgcggt caaggtctgc
     6361 cgtgtaggct tcgccgctcc cgcacgcatc gcccagcccg acgctccagg cgtgtccggc
     6421 cttgtccctg ccgctcaaca tcaactccgt ctcttccgtg taggcgagcg ttgccgtggc
     6481 gacgccgagg tcgagttgcc tgtgcggcga aggccgcgac tcaatcgctc cgaggtactt
     6541 cacggcgaaa ggccgcccca gttccgcgcc cacgagcggc aggtactcac gaaaggccgc
     6601 ctgcaccgcg acgcttgagg ccaaagtcgc ggccactaaa aagagcgcgg cggcgaactg
     6661 tctaaacttg aacatgcgtg aatctttcct tcctcgcgcg gaagagatac gaaaatccgt
     6721 gccggataat tcttactccg cccgcgcccg aacggtcaac gccgaggccg gccttgccct
     6781 ccgcacaaat atgatgacca aagccgtcgc gacggagtat gatgcgctcg tactcctaca
     6841 ctgaccggag atgctttagc aaacttccgt tgacgaccaa gccgggatta ttcgatagac
     6901 tcgcgaggta gccccgttcg tgcgcttctc cgcccttgtt acaatctgct cgctctgctg
     6961 cctgatttgc ctctcgcaaa ctgcttgcgg aagcgcgtcc acggaagccc gtgcgccgcg
     7021 cgccgcgacc ggaacggcgg cgcaggcgaa cgcgcccgaa gccgcacgca aaagcgaacc
     7081 ggtcgcaccc gtcgtcggtt cgattgacgc cgacgacgac gggctgcccg accgcgccga
     7141 gttgagttcc tacggcgacc gcgaaaattt tcgtcgctgg ttcaccgcgc tggccgagat
     7201 gcagtttcaa gagatcagca aacagtggaa cgccgagcag cgcgattgcg cggggctggt
     7261 gcgtttcagt tggcgcgaag ccctgcgcac gcacgaccgc gcgtggatgc aacgcatggg
     7321 cgacgcgtat gacgccatcg cgcccgacgt gcaccgcaca cgcacgctcg aacgcggcgc
     7381 gctcggcgcg agactttttc gcacccgccc cggcgcattt caggaacgcg acctgaccga
     7441 cggcaccttc tcggatttcg cagacgcgcg cacgctcaag gaattcaaca ccgagtttgt
     7501 cagccgcgac cgcaggcgcg cggagagcgg cgatctcatc ttcttccatc aaccgtgggc
     7561 gcaacaatac ccttatcacg tgatgatctt tctgggcgcg gcgcgcgagg cttcggaagg
     7621 cgcagccgat tgggtggtct atcacacggg cgcatcgccc ggcgaccggg gcacgatcaa
     7681 aaaagtgcgc ctcgcagtgc tcgacgagca cccggataga cgctggcgac ccgtccagtc
     7741 gaatcgcaac tttctgggct tctaccgcct caagattctc cgttgacaga agaacgactc
     7801 gcagttgaat gagacgacaa taagaaacga tgaatgaaaa gcaggagtca gaattcagca
     7861 tacaggagtc agaataaaag gtgcgcggct cttccttctg actcctgtgt tccaaattcc
     7921 ggattctgtg taatgcttta gtgaattgaa cgctctcgaa gggctgaacc tatgaaccgt
     7981 cgaaaactcc tgacttcgat cttgatctat ctggccgtcg cgtttgtcat cttctctttc
     8041 tcgttctggc ggctgcgcat gctcgccatc ggcacttcga tgggtgaagc cgcgaacgag
     8101 atcgaataca aagagcgcga cccgaacgtc ccggcgaagc ccttcttctc gctcaccacc
     8161 aaccgcacct acggcacgag cgaacgctcg cgcctctggc tcaactaccg cgacatagac
     8221 gccattgatt ttcgcgtcta tcgcgtgaac gaccccgcca agttctttcg cgcgctcgac
     8281 aacccgcacc agttcggcga agacgaagag ggcgagtttg agagtttcta tcgcaagcgc
     8341 aagcccactt tcctcgaacg cgtccgcgcc ttcaagaaca tcttctatct agggtttcgc
     8401 gactacttcc gcgcgcaact caagcacgaa tcgcgcaagc gtttcaatca gagattccgc
     8461 gcgcccgacg aactcaagga ggccgaagcg cagcgcacgc cgctgaacgt ggccgactac
     8521 gcgcgcgtgc cgcttctcaa cgaggatcaa ctcgtgcgcg gctggcgcga aaaactcccg
     8581 ccgctcgaca acgaatacga ccgtcgttcg atcccgctcg gtcaggacaa gccgggcgtc
     8641 tatctcgtcg aagccgtcaa cggcgacctg cgcgccttct gcgtcaccat cgtcaccgat
     8701 ctcacgatga tcgaaaagac ttccgccgac ggacagttgc tgctctatgc cgtcaaccgg
     8761 cagacgggcg cgccgcgcgc gggcgtacag gtgcaagtcc tgcaaggcaa agacacgctc
     8821 accaccggca ccaccgacaa tcaaggcatc ctgcgcacca aggtcgagcg gaagcagccg
     8881 aagagcgcgg aagaagcggc ggagggcgaa ggcgacgagg gcgcggaaga aagcgagggc
     8941 gcgagcgacg aagggggcga ggcttcctat ctcatcatgg cgacggagcg cgataatttt
     9001 gcgatctccg atctcgactc ctacatcttc ggcggctacg gcggcgaagg cgagaatctg
     9061 acgagttata tctacacaga ccgcccggtc tatcgccccg cgcagaaggt ctacttcaaa
     9121 ggcatcctgc gcacgctcgg cgagcgcggc taccagacgc ccggcggcac aatcacggtc
     9181 agcgtcgcgg acacgaacga gacgaagata ttcgagcggg agatgccgct ctcctcgcgc
     9241 ggcacgttca gcggcgaagt ggacatcccg gaagacgcgc cgctcggcac ttacaacgtt
     9301 accgcgacca ccgccaacgg cacggccagc ggctacttcg acgtgcagga atacaagaag
     9361 cccgaattca aggtggccgt ctccgtgccg caacgcttcg cgcccacggg ttcgcaggtg
     9421 aagttcaacg tctctgcgcg ctacttcttc ggctcgcccg tcacgcgcgc cgacgtgaag
     9481 tattacatct atcgcaagcg ttactatcat tacttctcca accccgaagc cgacccgatt
     9541 gacg
//
LOCUS       DASWNS010000260        21064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_88373, whole genome shotgun sequence.
ACCESSION   DASWNS010000260 DASWNS010000000
VERSION     DASWNS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 21064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21064
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88373"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VGB76_09185"
     CDS             complement(<1..356)
                     /locus_tag="VGB76_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEY0099113.1"
                     /translation="MAVRLSRTFVLRKLHQLSGIIPLGAFLLEHLYTNSKAAHPERGV
                     ADFNQAVADLQAIPYVLFIEITFIFIPLLYHAVYGLFITWETRPNNLSYPYPRNWFYT
                     IQRTTGIILFFFILFHV"
     gene            complement(518..1132)
                     /locus_tag="VGB76_09190"
     CDS             complement(518..1132)
                     /locus_tag="VGB76_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099114.1"
                     /translation="MTLYQTDSNLRAARERYFEINNFAGGGYEEGWVKMKAGFVPIWF
                     PNTVARVKAVRFHDIHHVLTEYPTTWSGEAEIGAWEVATGCAAHYQAWLLNLLAFAIG
                     LVINPRGVYRAFMRGRHSRNLYRTTFSDELLARTVGEMRRELELDKEVAPPTPSDRRA
                     FLFWSLASAATLLGTWLVALLPLLTLALIVVLLASRECVNFWRF"
     gene            1266..2159
                     /locus_tag="VGB76_09195"
     CDS             1266..2159
                     /locus_tag="VGB76_09195"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0099115.1"
                     /translation="MQIETLKIFCDLVETKSLSRAAERNFVTQSAVSQQVRGLEEKFK
                     RRLLERMRGGREVGLTEDGEVFYQESRQIVAAYVQLKERMRTLTGTVSGTVHVATVYS
                     IGLHELPAVIRRFMAEHPEAKIDLEYSRTTRIVRDVLAGTVELGIVAYPEKKRGLSVV
                     PLGGDRLVLICPPGHPLAKHKKLRARDLNNQEFVLFERDIPTRRAIDRIFRAHGVSVK
                     RVAEFDNIETIKRAVEVGFGAAIVPSPSVLDEERSGRLAVVPLAEAEWTRTSGVIYRS
                     DRTLGTAAKKFIQLLKEKSEK"
     gene            2179..3615
                     /locus_tag="VGB76_09200"
     CDS             2179..3615
                     /locus_tag="VGB76_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0099116.1"
                     /translation="MSAAQLCELIRRRALSPVEIMEAHLRRAEALNPVLNALVTFAPG
                     ALEEAHAAERAVMRGERLPPLHGLPVTIKDTIATANLRTTSGSRVRADHVPAADAPAV
                     ARLRAAGAIVFAKTNASELALDYNADNALFGRTGNPHDLSRTPGGSSGGCAAAVSACL
                     SPASLGSDLVGSIRIPAHFCGITGLFPTPGRVASLGHFPPTDGALAQGASLGPLARRV
                     DDLALLFDALSGKEEDSHDSSHANEFGQHVSETQLRGLRVAFYTDDGVVPVTTETKRA
                     VEAAARALTEAGLETIEARPPEISGATELWLALFSREVGQIVRAEYAGREELAGASVR
                     ALLRREEEVKATVSNAPGDRFEAWAERDRLRAALLERMRETPLLLAPVGASAAFAHGA
                     RKVASGRGELNTFRAFSYAQACNVFALPAVCVPSGRTREGLPIGVQIIGRPFREQLIL
                     AAARVIETALGGWQPPAIILPSGGVDPL"
     gene            3681..4697
                     /locus_tag="VGB76_09205"
     CDS             3681..4697
                     /locus_tag="VGB76_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0099117.1"
                     /translation="MEFFFFTFLLIIGIFLLVVAAKTIKIVPQSTVLLIERLGSFNRM
                     ASNGLNIIYPFIDKPRGVYWTNVRPGLTSIDLREQYIDLPPQPVITRDNVTIAVDSVV
                     YWQITDPVKAVYEVTDLVGGLVQLTITGMRSVMGEMDLDHTLSSRDQINAKLRIILDE
                     ATDKWGVKVTRVDVKNINPPEDVRLTMEKQMTAERNRRALVLQAEGDKQAAITRAEGE
                     KQASVTRSEGEKQSAILSAEGAAQARLRAAEAEAEAIQRIGEAMAQQAESAQYLITQR
                     YIESFKEMARGNNTKVIFMPMETSAMLSSVGAIKEMFAETGERSARKEERHNNPPPPR
                     QLPT"
     gene            complement(4779..5393)
                     /gene="thpR"
                     /locus_tag="VGB76_09210"
     CDS             complement(4779..5393)
                     /gene="thpR"
                     /locus_tag="VGB76_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY0099118.1"
                     /translation="MSDIQTKRSMRIFCAVDLPDDVRARAAAHISRLREAASATPIKI
                     GWERAEKLHLTVKFLGELEPERVEALMRAATRAAESVEKFALTLAEAGAFPSSGNPRV
                     LWLGLRDETKRLAALYERLEEECARESFPREARAFHPHVTIARIRIPNAAPARHLAKL
                     HRETGFEAASFNVGELIVMQSQLGAGGSRYTPLSRHLLKAETGG"
     gene            5883..6482
                     /locus_tag="VGB76_09215"
     CDS             5883..6482
                     /locus_tag="VGB76_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0099119.1"
                     /translation="MRKALFTALFFVFCAPMAFAQDDFQRGEFGVMFSHNRVDTDGAF
                     SNDPNDTGRDGFNGVNIDAGYNFSRYFGAKVDFSYHKKSNNVTIPAVGSFNVDGKLTQ
                     FMGGVKIQDNSTETKVRPFAQLLLGVGRVEVEASGTGIPTNFNESETGLAAAVGGGLD
                     IRVHRNVDIRAIKVEYNPVRIDGETGNNVRIGVGLNFRF"
     gene            complement(6679..9174)
                     /locus_tag="VGB76_09220"
     CDS             complement(6679..9174)
                     /locus_tag="VGB76_09220"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY0099120.1"
                     /translation="MKKLLLTSSLLLSFCFLHAFPQTFNLNAQPSATVAAPKQAAATT
                     LKVAGLRAPVQIRRDERGIPHIEASNEADLYFAQGYATASDRLWQMDLLRRTARGELA
                     EIFGRPVLEEDKRHRILGFGALSESLVAGVSPQTRATLEAYTRGVNAYIESRDGTDAK
                     ALPVEFQILGYKPRPWLPGDSLVIAKLFAETLSTTWPTDISRAMLADLPPARRAQLLP
                     ETSPLDVLVVGSDAPKSQPSPSPTRRRAAAKVRARSLPSFDGRERAALLEEVSRVNAL
                     SRISLERVGLFMEDRAASNNWVVSGARTASGKPLLANDPHLPASAPSIWHMAHLRAPG
                     LHVAGVTAPGAPGIIIGHNEQIVWGMTNLGPDVQDLYLEKFSADKKNYYETPAGLREA
                     EVRREEIKVRKGLTDTSTETVALDVTVTRHGPIILEREGKRYALRWTALDPKTNEFDA
                     FYAINRARNWNEFRAALASYTGPTQNFVYADTLGHIGYYGAGAIPIRQSGDGSVPYDG
                     ATDEGEWKSFIPFSELPHLYDPPSGLIVTANSRVVGRSYPYHLTHDWADPYRSRRIYD
                     LLSKGRKLTEEDFRAVQGDTYSIGGATFAREAAKLLRATTAKATPDEKFRAALALLEN
                     WDGYVNADSQAAPLVAEMRTAFRRRILTAALGAERSNAFRWTSSDLFFARVVSEHPAE
                     WLPKEFKDYGELMRACVEDALAALAKRAGANETPWTWGRYAPARFQHPLAVVPFIGRQ
                     FTIEPFPVNGSGFSAGATVNVGTSVSMRLIANLSDWDKTRQGIALGESGDPASPHWTD
                     QLADWRAVAPRLFPFSAKAISAATRETLLLAPK"
     gene            complement(9349..10578)
                     /locus_tag="VGB76_09225"
     CDS             complement(9349..10578)
                     /locus_tag="VGB76_09225"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0099121.1"
                     /translation="MFGQHVTHQLTPYAHGELSAPESSRVAAHLDACARCRLELAEIT
                     YAIQLAESLRLRSAPANLWDNIEAALAQESSRQREAERAAHAAAQATTPRRETQAQSS
                     RRSRRFGWDFGRVSGWRPALVASLFLLAMFGAGGAWLYLRATRTTWEVARLAGAPLIE
                     AARIHSTGRLGIGEWLETDANSRAQIKVADIGQVEIDPGTRIRLVETRLTEHRLELAR
                     GRMHATIYAPPRLFFVDTPSAVAADLGCAYTLEVDDKGRSTLHVTSGWVAFETKDRES
                     MVPAGAACLTQPGVGPGTPFFEDASALFLDALSKFDFDGGGADALHVVLNEARTRDTL
                     TLWHLLARVEGAERERVYERLATLAPPPASVTRQGIMLLDPSMLYDWKAQMEPDWLTE
                     SMPAVRNAWRSLWSDSK"
     gene            complement(10588..11166)
                     /locus_tag="VGB76_09230"
     CDS             complement(10588..11166)
                     /locus_tag="VGB76_09230"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0099122.1"
                     /translation="MIDERPMDTRVVASCQQGDREAFRLLFETHKDKVYSIALYFFGG
                     DSALAADITQQVFLKLFTRISQFRHQSEFTTWLYRLTTNTCIDEQRKRRRFSPLPDTL
                     PTQTATQGMPAAEARFMRLEVSDSVRDAIATLKPKLRIAILLKYFEELSYEEIAVVLN
                     CSKGTVASRLNRGHRILAQRLGHLRAGVSSGD"
     gene            11397..12275
                     /locus_tag="VGB76_09235"
     CDS             11397..12275
                     /locus_tag="VGB76_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099123.1"
                     /translation="MPFRRIHFIRFSSALVFTFICALAAAAQQRPLITEDVDIIPPGS
                     ARIEAGIEFFQDARFPVSGLRGDLTRVGVVGINIGLSPNVEFQIDGVLQNFLSINSAS
                     PNPAVPLNTARTDSTNDTGDFRLSTKIKLRNETRRAPSLGFRFGVELPNSSEGRGIGI
                     NQTNAYGTILLGKKFGREDRVNLFGNLGIGIFTAPTQSFTQNDMLLYGIAGIVRVNRQ
                     INIAGEINGRANTRGGRAPLGTESLSEARLGLQVKASGLRFDFAGIAGLTDFSPRSGV
                     TFGVTYDTPSIFTPVK"
     gene            12418..13131
                     /locus_tag="VGB76_09240"
     CDS             12418..13131
                     /locus_tag="VGB76_09240"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY0099124.1"
                     /translation="MLLLINGLGQWLRGVAEGLQRFIVEVPIYVAAPAMVIIGALDSS
                     LLSLPEINDYLVVMRCYSDPKAAFYFPLFAALGSVIGCMLLYVIMRRGGEVILRKRFR
                     ADHIERVERAYARFGFLTLAIPALLPPPMPFKIFVATAGALEYPRWRFMITIMIARSL
                     RYYVEGALAVYYGRQVLEFIEHYGLKMLGSVAALCVVGLAIYFIKNRLTGKKDVSTGE
                     PVVEPGEAQSDVVKVTSDE"
     gene            13237..13899
                     /locus_tag="VGB76_09245"
     CDS             13237..13899
                     /locus_tag="VGB76_09245"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY0099125.1"
                     /translation="MKWLLRKVTNGLTAVSQYLIAYGAFGLFGIALFDSALIPLPGGP
                     DAVLILLATAQPAWVPLYVFAATLGSVIGCLIFYYISRRAGKGALKRFSPRRQARVKE
                     LIERYDVLSVLVSSILPPPFPFKLFIVTAGVFRFSVVRFALAVAAGRAFRYALVGYLA
                     AHYGAHAKELLARYYPYIGLGLAVLIIAVFVLRNMFKKGGKGEEEAARARLAGESESE
                     LL"
     gene            complement(13885..14760)
                     /locus_tag="VGB76_09250"
     CDS             complement(13885..14760)
                     /locus_tag="VGB76_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY0099126.1"
                     /translation="MTHLSNLVLDCTFVQETVDLLRLSGGGRVPVEVIARTVLNLPDL
                     DPPTAALLVSDVIKDDWRLRINDNYEIELHCEDADCRALDETDYVVVDVETTGAKTPP
                     ARITEIGAYRVSRGRIVAEFQTLVNPQMPIPSFIVMLTGITDAMVKSAPLFSEVAQDL
                     LTFIDRAVLVAHNAPFDVRFLNHEISRVFPGKRMFNAQLCTVSLSRRVVPGLANHRLH
                     TVAEHFDVAIKNRHRAAGDAEATAEVFLRLLELLQQNGVRDLAAARKFKHANHDYQRA
                     SANGNAKPAGNSKQF"
     gene            14822..15406
                     /gene="pyrE"
                     /locus_tag="VGB76_09255"
     CDS             14822..15406
                     /gene="pyrE"
                     /locus_tag="VGB76_09255"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY0099127.1"
                     /translation="MRAAEVIERFRRTGALLEGHFILTSGLHSANYLQCALVLQHPRE
                     AEAFARAIAEQFAGRGIETVVAPAIGGLVIGYETARALGARSIWTERDAGGAMTLRRG
                     FTMRPGESVLVVEDVITTGGSTRETIECVRAAGASVVGAASIIDRSGGRADVSVPRIA
                     LATLDVPALAPYVCPACQRGEQAIKPGSRKLSNE"
     gene            15488..16255
                     /gene="truA"
                     /locus_tag="VGB76_09260"
     CDS             15488..16255
                     /gene="truA"
                     /locus_tag="VGB76_09260"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY0099128.1"
                     /translation="MNYRLTIQYDGTDFHGWQVQGAGERTVQGELVRALSLIEGREVV
                     VHGAGRTDAGVHAFGQVANVELRREISAERLCAAINGNIAPDARVIAAQAVDENFHAR
                     YSARGKTYRYRIFNARYASPFLARYTHTDARPLDVERMQACARLFPGEHDWTAFSAAQ
                     ADVQTRVRTITQLEVRERWSDAGRGRLVEIRASADGFLRYMVRSIAGTLLEVGRGQMD
                     AAQVTRAIESGERSLAGATAPAKGLTLLEVHYEDSDE"
     gene            16538..17329
                     /locus_tag="VGB76_09265"
     CDS             16538..17329
                     /locus_tag="VGB76_09265"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY0099129.1"
                     /translation="MLADFAEDVAAGTRDEALLKRVAWERLPRHVGIIMDGNGRWAKG
                     RGQPRIAGHRAGVEAVRAAVDTSARLGLSALTLYAFSTENWKRPRLEVDALMRMLKRY
                     LRLELEEIDRQNIRFQTIGRTGALPHSVRREIQKAVERTSKNDGLVLSVALNYGGRAE
                     IVDACRAAIARLVAAGRAPEEITEESIERELYTHGLPELDLLVRTSGEMRISNFLLWQ
                     AAYSEIYVTETLWPDFRRVHLLEAVVEYQRRDRRFGGLQLVANAN"
     gene            17364..17666
                     /locus_tag="VGB76_09270"
     CDS             17364..17666
                     /locus_tag="VGB76_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099130.1"
                     /translation="MFSFICIVITLLLLASIFWLIRRAGRRDSYGEGGSYYGGGDAGS
                     SFGGSGWGSDSGSQSNFGGGGDFGGGGSGGDFGGGDSGGGDSGGGDGGGGDGGGGE"
     gene            17765..18610
                     /locus_tag="VGB76_09275"
     CDS             17765..18610
                     /locus_tag="VGB76_09275"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY0099131.1"
                     /translation="MSRLITAIIALPVLIASILISQLELLFVVLAATAMMMGLYEFWM
                     LSSKRDAKAQRELGTLFSVALLTAFYFNAPFTWPALPLMILVVFVISTLAGAMLRGAN
                     FDRMILATGATVLGVCYVVLLGGHLVALRTGFPAPLAADLLSFFFLVLMGSDTAAYYT
                     GRAFGRHKLAPSVSPGKTWEGALGGLAASLGFAVLAHFWFFPELSLRVALPLAAAMNV
                     LGVLGDLTESALKRGSDAKDAAKILPGHGGLLDRLDSLLFNAPLIYYFAVFYFADALQ
                     RAQLP"
     gene            18656..19450
                     /locus_tag="VGB76_09280"
     CDS             18656..19450
                     /locus_tag="VGB76_09280"
                     /inference="COORDINATES: protein motif:HMM:NF020279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor Coa1 family
                     protein"
                     /protein_id="HEY0099132.1"
                     /translation="MSTKKILLIVGGIVAVLGLLVALFVGSIVWVAFSTIGKSEAAQT
                     AKSFLQQNEKLKSDIGEVRDFGFWVTGNINASNTNGEATLNLKAIGARKTVPASVNLA
                     YRNGREWIVVEASYRNDAGQTVDLLDVYERDATEPDEAGRGGATDNFGGTDSGETGAG
                     FDEESFSANVLQSEGTTLVVFLTQYSLDSQALEKTLDELSEEYAERVNLVRYSVDEQP
                     AVLQRFNIRQMPVIMVYRDGREQERRAGAISKQQLAALLDRYLETE"
     gene            19447..20631
                     /locus_tag="VGB76_09285"
     CDS             19447..20631
                     /locus_tag="VGB76_09285"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY0099133.1"
                     /translation="MNKRGIAILGSTGSIGCNTLRVIDAFAGDFQVVALAAGRNVREL
                     AEQTERYQPELISVETAEHADELRTELRRKGVAAPRILWGEAGLVEVATHERAACVVS
                     ATVGAVGFVPTLRALEAGKRVALANKETLVIAGELMTRAAQASGAELLPVDSEHNALH
                     QCLRGERREEVRRLVLTASGGPFRTKTLGEMEQATVAEAMRHPTWSMGAKITIDCATL
                     MNKGLEVIEARWLFGYEADQIDVLVHPESIVHSMIELVDGSVIAQMGVTDMRHAIQYA
                     LTYPERRACQLPPLDLTQMSALHFDSPDMERFPCLALAYRALRTGGTAPAALNAANEV
                     AVGAFLEERIRLTDIPRIIESVLDAHETRPVTDLDTVLETDAAARRSAADEIAGLVSV
                     IP"
     gene            20747..>21064
                     /locus_tag="VGB76_09290"
     CDS             20747..>21064
                     /locus_tag="VGB76_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0099134.1"
                     /translation="MTLLGFLAFIFILGSAVVLHEFGHFIVAKLFKMRVETFSVGFGP
                     RLFGRRWGTTDYRVSLIPLGGYVKLGGDESNAAVEGAGESDIPMNERFDLRPRWQKFL
                     VMVA"
BASE COUNT         4069 a         6324 c         6624 g         4047 t
ORIGIN      
        1 acgtggaaga ggatgaagaa gaagaggatg atcccggtcg tgcgctggat cgtgtagaac
       61 cagttgcgcg ggtaggggta gctcaaattg ttcgggcgcg tctcccaggt gatgaacaga
      121 ccgtaaaccg cgtgatagag cagcgggatg aagatgaacg tgatctcgat gaagaggacg
      181 taaggaatcg cttgcaggtc tgcgacggcc tgattgaagt ccgccacgcc gcgctccggg
      241 tgcgccgctt tcgagttggt gtagagatgc tccaacagaa acgcgccgag cgggatgatg
      301 cccgataact ggtgcagctt gcgcagcaca aaagttctac tcagacgaac agccatagct
      361 taagaatgac ccctttcgcc gctggtggac acagaagcgt gtgtgtcgcc gttttcgtgg
      421 cgggttggag cagacgccga agacgcgccc gacgctcagg attatttgga acggacatga
      481 ttataaagag cgcggccaaa aatgcaatcg ccgcctctta gaatctccaa aaattcacgc
      541 actccctgct tgccagcagc acgacgatca acgccagcgt caagagcggc agcagcgcga
      601 cgagccacgt ccccagcaga gtcgccgcgc ttgcgagcga ccagaaaagg aaagccctgc
      661 gatccgaagg cgtgggcggc gcaacttctt tgtccaactc caactcgcgc cgcatctcgc
      721 cgaccgtgcg cgcgagcaat tcgtcggaga aagtcgtgcg atacaagttc cggctgtgcc
      781 gcccgcgcat gaaggcgcga tagactccgc gcggattgat cacaaggccg atggcgaagg
      841 ccagcaagtt gagcagccac gcttgataat gcgccgcgca acccgtcgcc acttcccacg
      901 ctccgatctc ggcctcgccg ctccacgtcg tcggatattc agtcaacacg tgatgaatgt
      961 cgtggaacct gacggctttc acgcgcgcca ccgtgttcgg aaaccagatc ggcacgaacc
     1021 cggccttcat cttgacccaa ccctcttcgt agccgccgcc cgcgaaattg ttgatctcga
     1081 aatatcgctc gcgggcggcg cgcaggttcg agtcggtttg atagagagtc aaggggcggc
     1141 ctccttccgt tggcagcgaa caagtcggga aacgccattt tcggacgcta gtttaacccg
     1201 ccacggatat aaggtaaaat gaaaaatctt gcgccgatag ttgagttatt cttataataa
     1261 cggtcatgca aatcgaaact ttaaaaatct tttgtgatct cgtcgaaacg aaaagtttgt
     1321 cgcgcgcggc ggagcgaaac ttcgtgacgc agtcggcggt gagccagcag gtgcgcgggc
     1381 tggaagagaa gttcaagcgg cgtctgctgg aacgcatgcg cggcgggcgc gaagtggggc
     1441 tgacggaaga cggcgaagtt ttctatcaag agagccgcca gatcgtcgcc gcttacgtgc
     1501 aactcaaaga gcgcatgcgc actctgacgg gcacggtgag tggcacggtg cacgtcgcca
     1561 cagtctacag catcgggctg cacgagttgc ccgccgtcat ccgccgcttc atggcggagc
     1621 acccggaagc gaaaattgat ctggaataca gccgcaccac gcgcatcgta cgcgacgtgc
     1681 tggccgggac ggtcgaactc ggcatcgtcg cttacccgga gaagaaacgc ggcctctcgg
     1741 tcgtgccgtt agggggcgac cgcctcgtgc tcatctgccc gccgggacac ccgctcgcca
     1801 aacacaagaa acttcgcgca cgcgatctca acaatcaaga gttcgtactc ttcgagcgcg
     1861 acatcccgac gcggcgcgcc attgatcgaa tcttcagggc gcatggcgtg agcgtcaaac
     1921 gcgtcgcgga gtttgacaac atcgagacga tcaagcgtgc ggtggaggtc ggtttcggcg
     1981 cggccatcgt gccgagtccg agcgtgttgg acgaagaacg gagcgggcgt ctggccgtcg
     2041 tcccgctggc cgaagcagaa tggacgcgca cgtccggcgt catctatcgc agcgaccgca
     2101 cgctcggcac ggcggcgaaa aaattcatcc aactgctcaa ggaaaaaagt gagaagtaag
     2161 gacgacgaaa tcactgcgat gtctgccgcg caactgtgcg agttgatacg caggcgcgcg
     2221 ctctcgcccg tcgagatcat ggaagcgcac ttgcggcgcg cagaggcttt gaatccggtc
     2281 ttgaatgcgc tcgttacttt cgcgcccggc gcgcttgaag aggcgcacgc ggcggaacga
     2341 gcggtaatga gaggcgagcg tctgccgccg ctacatggcc tgcccgtgac gatcaaggac
     2401 accatcgcca ccgccaatct tcgcacgacg agcggttcgc gcgtgcgcgc ggatcatgtg
     2461 ccagccgcag acgctccggc agttgcgcgc ttgcgcgcgg caggcgcaat cgtcttcgcc
     2521 aagaccaacg cttccgagtt ggcgctcgat tacaacgctg acaatgcttt gttcgggcgc
     2581 accggcaacc cgcacgacct ttcgcgcacg cccggcgggt cgagcggagg ttgcgccgcc
     2641 gctgtgagcg cgtgcctttc gcccgcgagt ttaggcagcg atctcgtcgg ctcgatacgc
     2701 atccccgcgc acttttgcgg catcacgggt ctgttcccca cgccgggtcg cgtcgcaagc
     2761 ctcggccact tcccgccgac cgacggcgcg ctcgcacagg gcgcgagcct cggaccactt
     2821 gcccgccgcg tggacgacct cgcgcttctc ttcgacgcgc tctcaggcaa agaagaggac
     2881 tcgcacgatt catcacatgc gaacgagttc ggacagcacg tgtctgaaac tcaactgcgc
     2941 gggctgcgcg tcgctttcta cacggacgac ggcgtagtgc ctgtaacgac agagacgaag
     3001 cgagcggtcg aagctgccgc gcgcgccttg acggaggcgg ggcttgagac gattgaagcg
     3061 cgcccgccgg agatttccgg cgcgaccgaa ttgtggctcg cgctcttttc gcgtgaggtg
     3121 gggcagatcg tgcgcgcaga gtacgcaggg cgcgaggagt tggcgggcgc gtcggtgcgc
     3181 gctctgctca gacgcgagga agaggtcaag gctactgtct ctaacgcacc gggagatcgc
     3241 ttcgaggcat gggcggagcg cgaccgcctg cgtgccgccc tgctcgaacg gatgcgcgag
     3301 acgcctctgc tgctcgcgcc ggtcggagcg tctgcggcct tcgcacacgg ggcgcgcaaa
     3361 gtcgccagcg gaagaggaga gttgaacacg tttcgcgctt tcagctatgc gcaagcgtgt
     3421 aacgtgttcg ccctgcccgc cgtctgtgtc ccgtccgggc gcacgcgcga ggggctgccc
     3481 atcggagtgc agatcatcgg gcggccattt cgagagcaac tcattctggc cgccgcgcgt
     3541 gtgatcgaaa ccgcgctcgg cggctggcaa ccgcctgcga ttattcttcc atccggcggc
     3601 gttgatccgc tatagtgcgc acgcacaaat taacgttgac gcgctaacgt ctcgtgtcca
     3661 actcatctct ggaggcatct atggagttct tttttttcac cttcctttta ataatcggca
     3721 tcttcctgct ggtcgtcgca gccaaaacga tcaagatcgt gccgcagtcc acggtgttgc
     3781 tgatcgaaag gctcggcagt tttaaccgga tggcaagcaa cggcctcaac atcatctacc
     3841 cgtttattga caagccgcgc ggtgtctact ggacgaacgt gcgccccggc ctgacctcta
     3901 ttgatctgcg cgagcaatat atcgatctgc cgccacaacc cgtcatcaca cgcgacaacg
     3961 ttaccatcgc tgttgactcg gtggtctact ggcagatcac cgacccggtc aaagcggtct
     4021 acgaagtgac cgacctcgtg ggcggcctgg tgcagttgac gatcacgggc atgcgctcgg
     4081 tgatgggcga gatggatctg gatcacacgc tctcttcgcg cgatcagatc aacgccaaac
     4141 tgcgtatcat cctcgacgaa gcgaccgaca agtggggcgt gaaagtgacg cgcgttgacg
     4201 tgaagaacat caacccgccc gaggacgtgc gcctgacgat ggagaagcag atgacggcgg
     4261 aacgcaatcg ccgcgcactc gtcttgcagg cggagggcga caagcaggcc gccatcacgc
     4321 gcgcggaggg cgagaagcag gcttcggtga cgcgttcgga aggcgagaag cagtcggcga
     4381 tcctgtctgc ggaaggcgcg gcacaggcgc ggctgcgcgc cgcagaagcg gaggctgaag
     4441 ccatccagcg catcggcgag gcgatggcgc aacaggccga gtcggcgcag tatctcatca
     4501 cgcaacgcta catcgagagt ttcaaagaga tggctcgcgg caacaacacg aaggtcatct
     4561 ttatgccgat ggagacttcg gcgatgctgt cgagcgtcgg cgcgatcaaa gagatgttcg
     4621 cggagacggg cgaacgcagt gcacgcaaag aggagcgaca caacaatccg ccgccgcctc
     4681 gccagttgcc gacgtaagaa gagaagttag aggtcagagg ttaagaggcc ggaggataac
     4741 ggtcggggtt tcaactttga aatgtgaaac tccggcggtc atcctccggt ttctgctttc
     4801 aagaggtgtc tcgatagagg cgtgtagcgc gagccgcccg cgccgagttg gctctgcatg
     4861 acgattaact ctccgacgtt gaaactcgca gcctcgaatc ctgtctcgcg atgtagtttg
     4921 gcgagatggc gcgcgggggc ggcgttcggg atgcgtatgc gcgcgatggt gacgtgcggg
     4981 tgaaaggcac gcgcttcgcg gggaaaactt tcgcgcgcac attcctcttc cagtcgctca
     5041 tagagcgcgg cgaggcgttt cgtttcgtct cgcagaccta accagagcac gcgcgggttg
     5101 ccgcttgagg gaaacgcccc ggcctccgcc agcgtcaacg caaacttttc cacgctctcc
     5161 gccgcgcgcg tcgccgcacg catcaaggct tcaacccgct ccggttccaa ctcgcccaga
     5221 aacttcactg tgaggtgcag tttctccgcg cgctcccaac cgatcttgat cggcgttgcg
     5281 ctcgccgctt cacgcaggcg cgagatgtgc gcggcggcgc gtgcgcgcac gtcgtccggc
     5341 aaatccaccg cgcaaaaaat ccgcatgctt cgcttcgttt gaatatcgct catgtcaatt
     5401 ttggggttga actaaggcta aggcgtcggc gttctcaact tggagagggg gtgaccggaa
     5461 aacatcggcg cgctgaaaag tcggctcaca cggctccggt tggttccggt tatgaactct
     5521 ttaagaaatt tttaagaatc gctaaaagag tcccgcgttg ctctgcgtct agtattttcg
     5581 ctgccgggca atcgcctgaa tttacttcgt ccgtcatctg gattttcgga tcgcggacgg
     5641 cggctggtga ataaaaaact taatgaaagc gattagatag tttcatacaa cccggtgcaa
     5701 attatatgaa actaactccc tcgacaggtc gtttccctta gcttgtatcg ccgaaccgtc
     5761 ccggcacggc ttggaaagtt tgcaactgct ctacaggaaa cggcggtcgg gcaaccggat
     5821 gagggatgag tgaaaagcct tcatcaagat ttattagcat cacacggagg aaactaactc
     5881 acatgcgtaa agcattgttt acagctttgt tcttcgtctt ctgcgcgccg atggcgttcg
     5941 cgcaggacga tttccagcgc ggcgaattcg gcgtcatgtt ctcacacaat cgtgtggaca
     6001 ctgacggcgc gttcagcaac gaccccaacg atactggccg cgatggcttc aacggcgtga
     6061 acatcgacgc cggttacaac ttcagccgct acttcggcgc gaaggttgat ttctcgtatc
     6121 acaagaagtc gaacaacgtg acgattccgg ctgtcggctc gttcaacgtt gacggtaagt
     6181 tgacgcagtt catgggcggc gtcaagattc aggacaactc gacggagacg aaggttcgtc
     6241 cgttcgcgca actgctcctc ggcgtcggtc gggttgaagt cgaagcgtcg ggcacgggca
     6301 tccccacaaa cttcaacgag agcgagacgg gcttggccgc ggctgtcggc ggcggcctcg
     6361 acattcgcgt gcaccgcaac gttgacattc gcgcgatcaa agtggagtac aacccggtca
     6421 gaatcgacgg cgaaactggc aacaacgtcc gcatcggcgt cggtcttaac ttccgcttct
     6481 aagcgagact taaaactttg ccgggtaagc tgcccgtgtc tgaaaaggca cgggcagttt
     6541 tcatttccat gcttcgatag cggtgaagta ttaaggcact caccacagag acgcattccc
     6601 aaatcgcagc tcccggttcc cggtttaaaa acggcgcagg gacttgcgac ttgggacgcg
     6661 ctctgcctta tatagacttc acttcggcgc gagcaagagc gtctcacgcg tggcggcaga
     6721 gattgctttc gcgctgaagg gaaagaggcg cggggcgacg gcacgccagt cggcgagttg
     6781 atcggtccag tgcgggctgg cggggtcgcc cgattcgccg agcgcgatac cttgccgcgt
     6841 cttgtcccag tcgctcaaat ttgcgatgag gcgcattgag acgctcgtgc cgacgttcac
     6901 ggtcgcgccc gcgctaaagc cgctcccgtt gacggggaaa ggctcaatcg taaattggcg
     6961 accgatgaag ggcaccaccg cgagcgggtg ctggaaacgc gccggggcgt aacgccccca
     7021 cgtccacggc gtctcattcg cgcccgcgcg cttcgccaga gccgccagcg catcctcgac
     7081 acacgcgcgc atcaactcgc cgtagtcctt gaactccttc ggcaaccact ccgccggatg
     7141 ctccgatacg acgcgtgcga agaagagatc actgctcgtc cagcgaaacg cgttgctccg
     7201 ctccgcgccg agagccgcag tgagaatgcg ccgacggaag gccgtgcgca tctccgcaac
     7261 gagcggcgcg gcttgagaat cggcgttcac atacccgtcc cagttttcca gcagggcgag
     7321 cgcggcgcga aacttttcgt cgggcgtcgc cttcgccgtt gtcgcgcgca gcagcttcgc
     7381 cgcctcgcgt gcgaaggtcg ccccgccgat ggaataggtg tcgccctgca ccgcgcgaaa
     7441 atcttcctcc gtcaatttcc gccccttgct cagaagatcg tagatgcggc gcgagcgata
     7501 agggtcagcc caatcatggg tgaggtgata cgggtagctg cgcccgacga cgcggctgtt
     7561 ggccgtgacg atcagacccg acggcggatc gtacaggtgc ggcagctctg aaaagggaat
     7621 gaaactcttc cactcgcctt cgtctgtcgc gccgtcgtaa gggacgctcc cgtcgccgct
     7681 ctgcctgatg gggatcgcgc ccgcgccgta gtagccgatg tggccgaggg tgtctgcgta
     7741 gacgaaattt tgcgtgggtc ctgtgtagct ggcgagcgcg gcgcgaaatt cattccagtt
     7801 gcgcgcgcga ttgatcgcgt agaaggcgtc gaactcgttc gtcttcggat cgagcgcagt
     7861 ccagcgcaaa gcgtaacgct tgccctcgcg ttcgaggatg atgggaccat ggcgtgtcac
     7921 ggtcacgtcg agcgcgacag tctcggtcga agtgtcggtc agacccttgc gcaccttgat
     7981 ctcttcgcgt ctcacttcgg cttcgcgcaa gcccgccgga gtctcgtaat aattcttttt
     8041 atccgcgctg aacttttcga gatacaagtc ctgcacgtcg ggtccgaggt ttgtcatgcc
     8101 ccacacgatc tgttcgttgt ggccgatgat gatgccgggc gcgccgggcg cggtgactcc
     8161 ggcgacgtgc aggccgggcg cgcgcaggtg cgccatgtgc cagatggagg gcgcggaggc
     8221 gggcagatgc ggatcgttgg cgagcaacgg cttgcccgaa gcggtgcgcg cgccgctcac
     8281 cacccaattg ttgctcgcgg cgcgatcctc catgaacagc ccgacgcgtt cgagcgaaat
     8341 ccggctcagc gcgttgacgc gcgaaacctc ttcgagcaat gccgcgcgtt cccgtccatc
     8401 aaacgagggg agactgcggg cgcgaacttt cgcggcggct ctccttcgcg tcggcgacgg
     8461 agacggctgc gacttcggag catcgctgcc gacgacgagc acgtcgagtg gtgaagtttc
     8521 ggggagcagt tgcgcgcggc gcgcgggcgg gaggtctgcg agcatggcgc gcgagatgtc
     8581 cgtcggccag gtggtcgaga gagtttcggc gaacaacttg gcgatgacga gcgagtcgcc
     8641 cggaagccag gggcgcggct tgtagccgag aatctgaaat tcgacgggca gagctttcgc
     8701 gtcggtgccg tcgcgcgatt cgatgtaggc gttgacgccg cgcgtgtagg cttcgagcgt
     8761 ggcgcgcgtc tgcggcgaga cgcccgccac gagcgactcc gagagcgcgc cgaaaccgag
     8821 gatgcgatgt cgcttgtcct cttcgagcac ggggcgaccg aaaatctccg cgagttcgcc
     8881 gcgcgcggtg cgtcgcagca agtccatctg ccagaggcgg tcgcttgccg tcgcgtagcc
     8941 ttgcgcgaag tagaggtctg cttcgttcga cgcttcgatg tgcggaatgc cgcgctcatc
     9001 gcgacgaatc tgtaccggcg cgcgcaagcc cgcgaccttc aaggtggttg cagcggcttg
     9061 cttcggcgcg gcaacggtcg cggatggttg cgcgttgaga ttaaaagttt gcggaaaggc
     9121 gtgcagaaaa cagaacgaga gcaacagtga agaggtgagc agcagttttt tcatcgggtc
     9181 aaaatcagca cttctctctt ggagtttgga agacgctcgc gggcgtccgg gatggtaaca
     9241 cagaaacgaa atgatgaacg cggaacgaaa agcaggagtc agaaaacaga atacaggagt
     9301 cagaataagg cgtcagtcga taactttcat tctccctagc gggcatgttt actttgaatc
     9361 actccacagg ctgcgccacg cgttacggac ggcgggcatg ctctccgtta gccagtcggg
     9421 ttccatctgc gctttccaat cgtagagcat cgaagggtcg agcagcatga tgccttgccg
     9481 cgtcacactc gcgggcggcg gcgcgagcgt ggcgagacgt tcatagacgc gctcgcgctc
     9541 cgctccttcc acgcgcgcga gcaggtgcca gagcgtgagc gtgtcgcgcg tgcgcgcttc
     9601 gtttaagacg acgtgcaagg cgtctgcccc gccgccgtcg aagtcgaatt tcgagagcgc
     9661 gtccagaaac agcgccgaag catcttcgaa gaagggcgtg ccgggaccca cgcccggctg
     9721 cgtcaggcaa gccgcgcccg ccggaaccat cgactcgcga tccttcgtct caaacgccac
     9781 ccagcccgac gtgacgtgca gcgtgcttcg tcccttgtcg tcaacttcga gcgtgtaagc
     9841 gcagccgagg tcggcggcga cggcggaagg cgtgtccacg aagaagaggc ggggcggcgc
     9901 gtagatggtg gcgtgcatgc gcccgcgcgc cagttccaga cgatgctccg tgagtcgcgt
     9961 ctcgacgaga cggatgcgcg tgcccggatc aatctccacc tgtccgatgt cggccacctt
    10021 gatctgtgcg cgcgagttgg catccgtctc caaccactcg ccgatgccga gccgccccgt
    10081 ggaatggatg cgcgccgcct cgatcaacgg cgcgcccgcc agccgcgcca cttcccatgt
    10141 cgtgcgcgtc gcgcgcaggt ataaccacgc gccgcccgcc ccgaacatgg cgagcaggaa
    10201 gagactcgca actaaagcag gccgccagcc actcacccgt ccgaagtccc acccgaaacg
    10261 ccgactgcgg cgagaactct gcgcctgagt ttcgcggcgc ggagttgtcg cctgcgctgc
    10321 tgcgtgcgcg gcgcgctccg cttctctctg ccttgaagac tcctgcgcga gcgcggcttc
    10381 gatgttgtcc cagagattgg cgggcgcgga gcgcaacctc aaactttcgg ccagttgaat
    10441 cgcgtaggtg atctctgcca actcaagacg gcagcgcgcg cacgcgtcga ggtgtgcggc
    10501 gacgcggctc gactccggcg cggatagttc accgtgcgcg taaggcgtga gttgatgtgt
    10561 aacgtgctga ccgaacatct ctacttctta atctccactt gaaacgcccg cgcgcagatg
    10621 gccgagccgc tgcgcgagga tacgatggcc gcgattgaga cgcgaagcca cagtgccttt
    10681 ggaacaattg agaaccacgg cgatctcttc gtaagaaagc tcttcaaaat atttgagaag
    10741 gatggcgatg cgaagtttcg gcttgagcgt ggcgatggcg tcgcgcaccg agtcgctcac
    10801 ttcgagacgc atgaagcgcg cttcggcggc tggcatccct tgcgtcgcag tctgcgtcgg
    10861 cagcgtgtcg gggagcgggc tgaaacggcg tcgcttgcgc tgctcgtcaa tgcaggtgtt
    10921 ggtcgtcagg cgatagagcc acgtcgtaaa ctccgactga tggcgaaact gcgagatgcg
    10981 cgtgaagagt ttgaggaaga cctgctgcgt gatgtctgcg gcgagcgcag agtcgccgcc
    11041 gaaaaaatag agcgcgatgg aatagacttt gtctttgtgc gtttcaaaaa gcaggcgaaa
    11101 ggcttcgcgg tcgccctgct ggcaggaggc gacgacgcgc gtgtccattg gccgttcatc
    11161 aatcaccgcc cttagctccg tccccaaagc tgcattcaca aagctcacga cgcttagacg
    11221 gggcagcccg gaaatttctt ccaaaaaatt gtaacccggc gaggctccaa cattgacgct
    11281 ccgcaaacgg agccactaca atccgccttg agtcaggttt gaacgcgggc aaggctgctt
    11341 caaaagtccg tcacacttca aaagtagaag attcgtctcc gaccgttaac atgcttatgc
    11401 cgtttcgtcg catccacttt atacgctttt cgtccgcgct tgtgttcacc ttcatttgtg
    11461 cgctcgccgc cgccgcgcag caacgcccgc tcatcaccga agacgtggac atcatcccgc
    11521 ccggttcggc gcgcatcgaa gcgggcatcg agttttttca ggacgcgcga tttcccgtct
    11581 cgggtttgcg cggcgaccta acgcgcgtcg gcgtcgtcgg catcaacatc gggctttcgc
    11641 cgaacgtcga gtttcaaatc gacggcgtgc tgcaaaattt cctgagcatc aactcggcct
    11701 cgcccaaccc ggctgtgccg ctcaacaccg cgcgcaccga ttccaccaac gacacgggag
    11761 attttcgcct ctcgaccaag atcaaactgc gcaacgagac gcgacgcgcg ccttcgctcg
    11821 gtttccgctt cggcgtcgaa ctgccgaatt ccagcgaggg gcgcggcatc ggcatcaacc
    11881 agacgaacgc ttacggcacg atcctgttag gtaaaaagtt cgggcgagaa gatcgcgtca
    11941 atctcttcgg caatctcggc atcggcatct tcaccgcgcc cacgcagagt ttcacgcaga
    12001 acgacatgct gctctacggc atcgcaggaa tcgtccgcgt caaccgccag atcaacatcg
    12061 cgggcgagat caacgggcgc gccaacacgc gcggcggacg cgcgccgctc ggcacggaat
    12121 cgctctcgga agcgcgtctc ggcctgcaag tcaaagcctc cggcctccgc ttcgacttcg
    12181 cgggcatcgc cggtctgaca gatttcagcc cccgctccgg cgtcaccttc ggcgtcactt
    12241 acgacacccc ttccatcttc actcccgtca aatagaacag tgacgagtgg cgagcacgaa
    12301 agacgtagat cgtgaatcgt caatagagga aaactgtttt actctattca cgatttacga
    12361 ttcacgattt acggtttcaa tcttgtcact cgacgcttat cactcatcac tgtttttatg
    12421 cttcttctga tcaacgggtt gggacaatgg ctgcgcggag ttgcggaggg gctgcaaaga
    12481 ttcatcgtcg aagtgcccat ctatgtggcc gcgcccgcga tggtcattat cggcgcgctc
    12541 gattcgtcgc ttctttcgct ccccgaaatc aacgattatc tggtcgtgat gcgctgctac
    12601 agcgatccga aggcggcctt ctattttccg ctcttcgccg cgctcggttc ggtcatcggc
    12661 tgcatgttgc tctacgtgat catgcgtcgc ggcggggaag tcatcctgcg caaacgcttt
    12721 cgcgccgacc acatcgaacg cgtcgaacgc gcctatgcgc gcttcggatt cctgacgctc
    12781 gccatccccg cgctcctgcc gccgccgatg cctttcaaaa tcttcgtcgc cacggcgggc
    12841 gcgcttgaat acccgcgctg gcgtttcatg atcactatca tgatcgcgcg cagcctccgc
    12901 tactacgtcg aaggcgcgct cgccgtctat tacggcaggc aagtgcttga gttcatcgaa
    12961 cactacgggt tgaagatgct cggctcggtc gccgcgctgt gcgtcgtcgg gctggcgatc
    13021 tatttcatca agaatcgcct gacgggaaag aaggatgtgt cgactggcga gcctgtcgtc
    13081 gagccgggcg aggcacaaag cgacgttgtt aaagtgacga gtgacgagtg acgagttaaa
    13141 accaaatacc cgtcctgcac tcgtctcttg tccttaactt gtcactcgtc acttgtcact
    13201 tgtctctcgt cactgctttt ggaggttagt tgagttttga aatggctgct gcggaaagtt
    13261 actaatggcc tcacggctgt ctcgcaatat ttaattgcct acggggcttt cggcctcttc
    13321 ggcatcgcgc tcttcgattc cgcgctcatc cctctgcccg gtggtcccga cgccgtgctg
    13381 atcctgcttg cgacggcgca acccgcgtgg gttcccctct acgtcttcgc ggcgacgctc
    13441 ggctcggtca tcggttgtct gatcttttat tacatctcgc ggcgcgcggg taaaggcgcg
    13501 ttgaaacgtt tttcgccgcg caggcaggcg cgggtgaaag agttgatcga gcgttacgat
    13561 gtgctgtcgg tgctcgtctc gtcaattttg ccgccgccgt ttccgttcaa attgttcatc
    13621 gtgacggcgg gcgtctttcg tttcagcgtc gtgcgtttcg cgctcgccgt cgccgccgga
    13681 cgcgcctttc gctacgcgct cgtcggctat ctggcggcgc actacggcgc gcacgccaaa
    13741 gagttgctcg cgcgatacta cccctacatc ggactcggtt tggccgtgct catcattgcc
    13801 gtcttcgtct tgcgaaacat gtttaagaag ggcggcaagg gcgaagagga agccgcacgc
    13861 gcgcggctcg cgggcgagtc ggaatcagaa ctgctttgag ttgcccgccg gctttgcgtt
    13921 gccgttggcg gaggcgcgtt gatagtcgtg gttggcgtgt ttgaatttgc gcgcggcggc
    13981 cagatcgcgg acgccgtttt gctggagaag ttcgagcagg cgaaggaaga cttcggcggt
    14041 ggcctctgcg tctccggcgg cgcggtggcg atttttgatc gccacgtcga agtgttcggc
    14101 gacggtgtgc aggcgatggt tggcaagtcc gggcacgacg cggcgcgaga gcgagacggt
    14161 gcagagttgc gcgttgaaca tgcgcttgcc gggaaagacg cgcgagattt cgtgattgag
    14221 aaagcgcacg tcgaagggcg cgttgtgcgc gacgagcacg gcgcggtcga tgaaggtgag
    14281 aaggtcttgc gcgacttccg agaagagcgg cgcggatttg accatcgcat ccgtgatccc
    14341 cgtcagcatg acgatgaagg aggggatcgg catctgcggg ttgaccagcg tttgaaactc
    14401 ggcgacgata cgaccgcgac tgacgcgata cgctccgatc tccgtgatgc gcgccggggg
    14461 agttttcgcg cccgtcgtct cgacatcaac caccacgtag tccgtctcgt cgagcgcgcg
    14521 gcaatccgcg tcttcgcagt gcagttcgat ctcgtagtta tcgttgatgc gcaaacgcca
    14581 gtcgtctttg atcacgtcgg agacgaggag cgcggcggtg ggcggatcga ggtcgggcag
    14641 gttgagcacc gtgcgtgcga tcacttcgac aggcacacgc ccgccgcccg acagtcgcag
    14701 cagatcaact gtttcctgta cgaaggtgca atctaagacg aggttcgaaa ggtgtgtcat
    14761 ccgcgcggca ttgtagcacg gagcgcgccg gttgggagga acaaatggga ggagctacag
    14821 gatgcgtgca gcagaggtga ttgaaaggtt ccggcgcaca ggcgcgttgc tcgaaggcca
    14881 tttcatcctg acgagcggac tgcacagtgc aaattatttg caatgcgctc tcgtcctgca
    14941 acacccgcgc gaagccgaag ccttcgcgcg cgccatcgcc gaacagtttg ccgggcgcgg
    15001 catcgagacg gtggttgcgc ccgccatcgg cggcctcgtc atcggctacg aaacggcgcg
    15061 cgcgctcggc gcgagatcaa tctggacgga acgcgacgcg ggcggcgcga tgacgctccg
    15121 gcgcggcttc accatgcgac cgggcgagag cgtcctcgtc gtggaagatg tcatcacgac
    15181 gggcggttcg acgcgcgaga caatcgagtg cgtgcgcgcc gccggggcaa gcgtcgtcgg
    15241 cgcggcctcg atcatagatc gttcgggcgg gcgcgcagac gtaagcgttc cgcgcatcgc
    15301 cctcgccacg ctcgacgtgc ctgccctcgc cccttacgtc tgccccgcct gccagcgcgg
    15361 cgagcaggcg attaaaccgg gaagcaggaa attaagtaat gagtgatgag taatgagtga
    15421 tgagttgaaa gacaaagttt ttgttttaac tcatcactca ttactcatca ctcattactt
    15481 gttatttatg aattatcggc tgaccattca atacgacggg acggattttc atggctggca
    15541 ggtgcagggc gcgggggagc gcacggtgca gggcgagttg gtgcgcgctc tgtctttgat
    15601 cgaggggcgc gaggttgtcg tgcacggggc gggacggacg gatgcgggcg tgcacgcgtt
    15661 tggacaagtg gcaaacgtcg agttgaggcg cgagatcagc gcggagagat tgtgcgcggc
    15721 gatcaacggg aacatcgcgc cggatgctcg cgtcatcgct gcgcaggcgg tggacgaaaa
    15781 ctttcacgcg cgctattcgg cgcgggggaa gacttaccgt tatcgcatct tcaacgcgcg
    15841 ttatgcgtcg ccgtttctgg cgcgctacac gcacacggat gcgaggccgc tcgacgtgga
    15901 acggatgcag gcgtgcgctc gcctctttcc cggcgagcac gattggacgg cattttccgc
    15961 tgcgcaggca gacgtgcaga cgcgcgtgcg taccatcacg caactcgaag tgcgtgagcg
    16021 ttggagcgat gcgggacgtg ggcggctggt cgaaatcagg gcgagcgcgg acggcttcct
    16081 gcgctacatg gtgcgctcaa tcgcgggcac gctgctcgaa gtcgggcgcg ggcagatgga
    16141 cgcggcgcag gtcacgcgcg ccatcgaaag cggcgaacgc tcgctcgcgg gcgcaaccgc
    16201 cccggccaaa ggcttgacgc ttctggaagt ccactatgag gacagtgacg agtgacgagt
    16261 gataagtgac gagcgacagg ccggaagcct gtagagcgtg aatcataaat cgtgaagaga
    16321 ttcaaaaacc gtgaatcgtg aatcgtaaat cgtgaagaga agaagagaag gagagaagac
    16381 gggttttctc tatttacgat tcacgattta cgattcacgg ttttttctcg tcactcgtca
    16441 ctcttgcggt gttgcgtggg agtgcgtgcg acgattaaga tgcggcgtcc tgtgtcggcc
    16501 tgtttgagcc ggcgttgagg cgtcaggggg agtgttaatg ctcgcggatt tcgcggaaga
    16561 tgtggcggcg gggacgcgcg acgaggcttt gttgaagcgc gtcgcgtggg aacgcttgcc
    16621 gcgccacgtc ggcatcatca tggacggcaa cgggcgttgg gcgaaggggc gcgggcaacc
    16681 gcgcatcgcc ggacatcgcg cgggcgtcga agccgtgcgc gccgccgtcg atacgagcgc
    16741 gcggctcgga ctgagcgcgc tcacgctcta cgctttctcc accgagaact ggaaacgccc
    16801 gcgcctcgaa gtggatgcgc tgatgcgaat gctgaaacgt tacctgcggc tcgaactcga
    16861 agagattgac cggcagaaca tacgctttca aaccatcggg cgcacgggcg cgctcccgca
    16921 tagcgtccgg cgcgaaattc aaaaggccgt cgagcgcacg tcaaagaacg acgggctggt
    16981 tctctccgtc gcgctcaact acggggggcg cgcggaaata gttgacgcct gccgcgccgc
    17041 catcgcgcgc ctcgtggccg cagggcgcgc ccccgaagag atcacagaag agagcatcga
    17101 gcgcgagctt tacacgcacg gcctccccga actcgacctg ctcgtgcgca cgagcggcga
    17161 aatgcgtatc tcgaattttc ttctctggca ggccgcctac agcgagattt acgtgaccga
    17221 aactctgtgg cctgattttc gccgcgtgca tctgctcgaa gccgtcgtcg agtatcaacg
    17281 ccgcgaccgg cgcttcggcg ggctgcaact cgtcgcaaac gcgaactaaa agaagcgcct
    17341 tccggtctga caggagactc gtcatgttca gttttatctg catcgtcatt accctgctac
    17401 tgctggcatc gatcttctgg ctcatcaggc gcgcgggcag gcgcgattca tacggcgagg
    17461 ggggaagcta ctacggcgga ggcgacgcgg gcagcagctt cggcggaagc gggtggggca
    17521 gcgactccgg cagccaaagt aacttcggcg gcggaggtga tttcggcggc gggggcagcg
    17581 gcggcgattt cggtgggggc gacagcggcg gaggcgatag tggcggaggt gacggcggcg
    17641 gaggtgatgg cggcgggggc gaatgataga cgccacgagc gatctccgcg tcgctcgcgc
    17701 ttaacgcatc aactcgcagt tgagtctaag gttctcgttt tcagtctcag ttcatccgaa
    17761 aataatgtcg cgtctcatca cagccatcat cgccctcccc gttctcatcg cctcaatcct
    17821 gatcagtcaa ttggaacttc tcttcgtcgt gctggcggcg acggcgatga tgatggggct
    17881 ttacgagttc tggatgcttt cgagtaaacg cgacgcgaaa gcgcaacgcg aactcggcac
    17941 gctcttcagc gtcgcactgc tcacggcctt ctacttcaac gcgcccttca cctggcctgc
    18001 gctgcccctc atgattctcg tcgtcttcgt catctcgacg ctcgcgggcg cgatgctcag
    18061 gggcgcaaac ttcgaccgca tgatccttgc gacgggcgca accgtgcttg gcgtctgcta
    18121 cgtcgtcctg ctcggcgggc atctcgtcgc gctgcgcacg ggattccccg cgccgctcgc
    18181 agccgatctg ctctccttct tcttcctcgt gctcatggga tcggacacgg cggcctatta
    18241 caccgggcgc gccttcggtc gccataaact cgcgccctcg gtcagtccgg ggaagacttg
    18301 ggaaggcgcg ctcggcggac tcgcggcgag cctcggcttc gccgtcctcg cgcacttttg
    18361 gttcttcccc gaactcagtc tgcgcgtggc cttgccgctc gcggcggcga tgaatgtgct
    18421 cggcgtgctc ggcgacctga cggagagcgc gctcaaacgc ggatcggatg cgaaggacgc
    18481 ggcgaagatt ctccccggcc acggcggtct gctcgacagg ctcgatagct tgctcttcaa
    18541 cgcgccgctc atctactatt tcgccgtctt ctattttgcc gacgcactac aacgcgcgca
    18601 gctgccttaa ctactttgca ccgcgccaga atttttccca cggaaccaac tgtgcatgtc
    18661 aactaaaaag attctgctca tcgtcggcgg aatagtcgcc gtgctcggat tactggtcgc
    18721 gctcttcgtc ggcagtatcg tgtgggtcgc cttctccacc atcggcaaga gcgaagccgc
    18781 gcagacggcg aagagctttt tgcagcagaa cgagaaactc aaaagcgaca tcggcgaggt
    18841 gcgcgacttc ggcttctggg taacgggcaa catcaacgcc agcaacacca acggcgaagc
    18901 gacgctcaat ctgaaagcca tcggcgcgcg caagaccgtc ccggcgagcg tcaatctggc
    18961 gtacaggaac gggcgcgaat ggatcgtggt cgaagcctcc tatcgaaacg acgcggggca
    19021 gacggtggac ttgctcgacg tttatgagag ggacgcgaca gaaccggacg aagcggggcg
    19081 cggcggcgcg acggacaatt tcggaggcac ggattcgggc gagacgggcg caggcttcga
    19141 cgaagagagt ttttccgcca acgtcttgca gtcggagggc acgacgctcg tagtcttcct
    19201 tacgcagtac agtctggaca gtcaagctct ggagaagact ctggacgagt tatcagaaga
    19261 gtatgcggag cgcgttaatc tcgtccgtta tagtgtggac gagcagcccg ccgtgcttca
    19321 gcgtttcaat atccggcaga tgcctgtcat catggtgtat agagacggca gggagcagga
    19381 acggcgcgcc ggggcgatct cgaagcaaca actggccgcg ctgctcgaca ggtatctgga
    19441 aaccgaatga acaaacgggg catcgcgatc ttaggttcga ccggatcaat cggctgcaac
    19501 acgctgcgcg tgattgacgc cttcgccgga gactttcagg tggtcgcgct cgccgccggg
    19561 cgcaacgtcc gcgaactcgc cgagcagacc gagcgttacc aaccggaact catctccgtc
    19621 gaaaccgcag aacacgcgga tgagttgcgc accgaactca gacgcaaggg agtcgccgcg
    19681 ccgcgcatcc tctggggcga ggcggggctg gtcgaagtcg ccacgcacga acgcgccgct
    19741 tgcgtcgtca gtgcgacggt gggcgcggtg gggttcgtgc cgaccctgcg tgcgctcgaa
    19801 gcggggaagc gcgtcgcgct cgccaacaag gagacgctcg tgatcgcggg cgagttgatg
    19861 acccgcgcgg cgcaggcgtc gggcgcggaa ctcctgcctg tcgattccga acacaacgcg
    19921 ctccatcaat gcctgcgcgg cgagcggcgc gaagaggtgc gacgactcgt gctcacagcc
    19981 tccggcggcc cctttcgcac gaagacgctc ggcgagatgg agcaggccac cgtcgccgaa
    20041 gcgatgcgtc atccgacctg gagtatgggc gcgaagatca cgattgattg cgcgacgctg
    20101 atgaacaagg ggttggaagt gatcgaggcg cgctggctct tcggctacga ggcagaccag
    20161 attgacgtgc tcgtccatcc cgaatcaatc gtccattcga tgatcgaact cgtcgatggc
    20221 tcggtcatcg cgcagatggg cgtcacggac atgcgccacg ccatacagta cgcgctgacc
    20281 tatcccgaac ggcgcgcgtg ccaacttccc ccgctcgacc tgacgcagat gtccgcgctc
    20341 catttcgact cgcccgacat ggagcgtttt ccctgtctcg ccctcgccta tcgcgccctg
    20401 cgcacgggcg gcaccgcgcc cgccgcgctg aacgccgcca acgaagtcgc cgtcggcgcg
    20461 tttctcgaag agcgcatccg cctgacagac attccgcgca tcatcgaaag cgtgctcgac
    20521 gcgcacgaga cgcgccccgt cacagacctc gacaccgtgc ttgagacgga cgcggcggcg
    20581 cggcgcagcg cggcggacga gatcgcgggt ctggtttcgg taatccctta gtttgacgcg
    20641 cttagctcaa ccccgtaaac tcttgacgtg caaccgggtg agcgcatatt gcctctaagt
    20701 tgcgcggcaa tttggtggtt tcaccggaga aagacgttaa aaaagcatga ccttactagg
    20761 atttctcgcc ttcatcttca ttctcggctc agccgtcgtg ctccacgagt tcggccactt
    20821 catcgtcgcg aagctcttca aaatgcgcgt cgagacgttt tcggtcggct tcgggccgcg
    20881 cctcttcggg cggcgttggg gcacgaccga ctaccgcgtc agcctcatcc cgctcggcgg
    20941 ctacgtcaaa ctcggcggcg acgaatcgaa cgccgccgtc gaaggcgcgg gcgagagcga
    21001 catccccatg aacgagcgtt tcgacctgcg cccgcgctgg cagaagtttc tcgtcatggt
    21061 ggcg
//
LOCUS       DASWNS010000261         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_36329, whole genome shotgun sequence.
ACCESSION   DASWNS010000261 DASWNS010000000
VERSION     DASWNS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36329"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VGB76_09295"
     CDS             complement(<1..421)
                     /locus_tag="VGB76_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099135.1"
                     /translation="MFVSLTQYIFASLVALALTPACAPVETPHASVANVASTEVLIES
                     APEAAQGDDALSNARRLDRADRAADGQLAQLAPAEHMRRAAAYHANRAFAEAREHWQT
                     LIARHPADANVPAALFGMGRTFYQERRYAEALPFFDRL"
     gene            complement(624..776)
                     /locus_tag="VGB76_09300"
     CDS             complement(624..776)
                     /locus_tag="VGB76_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099136.1"
                     /translation="MADETKKCAHAACDCPADDNSKYCSAYCEGKGQTTTIDCDCGCP
                     SCGSNI"
     gene            complement(897..1343)
                     /locus_tag="VGB76_09305"
     CDS             complement(897..1343)
                     /locus_tag="VGB76_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY0099137.1"
                     /translation="MMNSEDARATIRYAGDDLFVAITPSGHAQTLDTRHERSAAATPM
                     ELLLLALGSCTAVDVISILNKKRERVTDYRVEVRGERRAEHPRKYTRFEVRHIVRGHE
                     VSAKAVAQAIELSETKYCSVAATLSPGAEIVSSFEIQEEEAGDAAP"
     gene            1799..>2929
                     /locus_tag="VGB76_09310"
     CDS             1799..>2929
                     /locus_tag="VGB76_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099138.1"
                     /translation="MTRARQIFSAWMVLALLSLSFGVQAQTTRRRPVYRNNQVQTQQL
                     LRRLETNAERFRLSLSQAMDQSRIDNTRREENISSVLDDFNHIVDHVRERYDQRQLVA
                     ADVEALLARASRLDRFMTNQSNRLSVRAQNDWASLRTDLDQLAGMYNVAWNRTNNAPY
                     NPYPPTDNNYNYDTGLTGTYRLNVAQSEDPRREAQLITNTLPARDRRRTYDAVVARLE
                     PPTEIAIERRGDNVTIASTRAPQINFIADGSERVEQGTYGNTVRARAAFNGDQLTVST
                     TGNRDNDFAVTFDPINNGRRLQVTRRITMTNVSQPIVVRSVYDRTADVARFDVYRGGQ
                     GYPTNTVGGSSGDFVITNGTTLIATLNNDLNTRNSREGDRFTI"
BASE COUNT          607 a          878 c          891 g          553 t
ORIGIN      
        1 caagccggtc gaaaaacgga agtgcctcgg cgtagcgtcg ttcctgataa aaggtgcgac
       61 ccatgccgaa gagcgcagcc gggacgttcg cgtccgcagg gtggcgcgcg atgagcgtct
      121 gccagtgttc gcgcgcttcc gcaaaggcgc ggttggcgtg ataggcggcg gcgcggcgca
      181 tgtgttcggc gggcgcgagt tgtgcgagtt ggccgtctgc ggcgcggtct gcgcggtcca
      241 ggcggcgcgc gtttgagagg gcatcgtcgc cctgcgcggc ttcgggggcg gactcaatca
      301 ggacttccgt gctcgcaacg tttgcgacgg acgcgtgcgg cgtttcgacg ggagcgcagg
      361 ccggagtgag cgcgagcgcg acgaggctgg cgaagatgta ttgagtcaaa gagacaaaca
      421 taggtatctt gatcgcaaag tcaggaatat agaatcgacg cctgcttcgt gtggaagcgg
      481 agagagttgg cgcgcggatc gttcgatgcc ggcacggcgc gacgcgtgcg ccgcccgtcg
      541 ttcggggaga gcttgaaggc ggaacggctg acgcgcgcct gctctcggaa ttgagcggag
      601 cgcacgccag ccgttcgggg aagttagatg ttgctgccgc aggacgggca gccgcaatcg
      661 caatcgatgg tcgtcgtctg gcctttgccc tcgcaatagg cgctgcaata cttgctgttg
      721 tcgtccgccg ggcaatcgca ggcggcatga gcgcatttct ttgtctcgtc agccatttct
      781 ttgtctctcc ttagtggtag ttgctggtca gtgcgtgcgc ttattctagc agcgaaacgg
      841 ctggcggcgc acgcgtccgc tgcaaacgaa gcgcacgacg cgacgacgcg atgacttcaa
      901 ggcgcggcgt ctcccgcttc ctcttcctga atctcgaaac tcgaaacaat ctctgcgccg
      961 gggctgagcg tggcggcgac gctgcaatac ttcgtttcgg aaagttcgat ggcctgcgcc
     1021 acggctttcg ccgaaacttc gtgcccgcgt acgatgtgcc gcacttcaaa gcgcgtgtac
     1081 ttgcgcgggt gctccgcgcg tcgttcgccg cgcacttcga cgcgatagtc cgtcacacgc
     1141 tcgcgcttct tgttgaggat cgagatgaca tcaaccgccg tgcaactgcc gagcgcgagg
     1201 agcagcagtt ccatcggcgt cgcggcggcg gagcgttcgt gtctggtgtc gagcgtctgc
     1261 gcgtggccgc tcggtgtgat ggcgacgaag aggtcgtcgc ccgcgtatcg aatcgtcgct
     1321 ctggcatctt ccgaattcat catgtagctc cttatatccc gctcacggaa aagcatagca
     1381 cgggagccga aaagaggctt atcgcgcccg cgcgcacacg aattgcatgt attcaagcgg
     1441 tgggaggatg ttcctacggt tgcagtggtt aaaaggccat tccgcgggca ttacagtgac
     1501 tttgcctgat acggataagc atgcagtgtg cacgatcttt gtacataaat tgtcagcatc
     1561 aatagcgcgg aaagtctctt gcgctgcaac ctgaaaaatt ggcggcaacc ttcgcaaggt
     1621 agtctcgaat ctcatttttg aacgcaaacg taaggtttgc atctctcctc tttttcaaaa
     1681 catcactccc cccacaatac ggaaatcacg aggcgcgtcc cgcgtggacc tttagccctc
     1741 gtgagaagtt tggaagatga ctgaagagtg aaacaagcaa aatggaggga agagcattat
     1801 gactagagca agacaaatat tttcggcatg gatggtgctg gcgctactga gcctcagctt
     1861 cggcgtgcag gcgcagacga cgcgccgccg tcccgtctat cgcaacaatc aagttcaaac
     1921 gcaacaactc ctcaggcgtc tggaaaccaa tgcggagcgg ttccgtttga gtttgagcca
     1981 ggcgatggat caaagtcgca tcgacaacac gcgtcgtgaa gagaacatca gctcggtgct
     2041 cgacgatttc aaccacatcg tagatcacgt gcgcgagcgt tacgaccagc ggcagttggt
     2101 cgccgccgat gtcgaagccc tgttggcgcg cgcttctcgc ctcgacaggt ttatgacgaa
     2161 ccagagcaat cgcctgagcg tccgcgcgca gaacgattgg gcttcgctca gaaccgacct
     2221 cgaccaactc gcggggatgt acaacgtggc gtggaatcgc accaacaacg cgccctacaa
     2281 tccttacccg ccgaccgaca acaactacaa ctacgatacg gggctgaccg gaacctatcg
     2341 cctcaacgtg gcgcagagcg aagacccgcg ccgtgaagcg caactcatca ccaacactct
     2401 gcccgcacgc gaccgtcgcc gcacttatga tgcggtcgtc gcgcgccttg agccgcccac
     2461 ggagatcgcc atcgaacgac gcggcgacaa cgtgaccatc gcttccacgc gcgctccgca
     2521 gatcaacttc atcgccgacg gcagcgaacg cgtcgaacag ggcacctacg gcaacacggt
     2581 gcgcgcccgc gccgccttca acggcgacca gttgaccgtg agcacgacgg gaaaccgcga
     2641 caacgatttc gccgtcacgt tcgacccgat caacaacggt cgtcgcttgc aggtgacgcg
     2701 ccgcatcacc atgacgaatg ttagtcaacc catcgtcgtg cgcagcgtct acgaccggac
     2761 agccgacgtg gcgcgcttcg acgtttacag gggcggtcaa ggctacccga caaacaccgt
     2821 cggcggtagc agcggcgatt tcgtcatcac caacgggacg acactcatcg ccacgcttaa
     2881 caacgacctc aacacgcgca actcgcgcga gggcgaccgc ttcacgata
//
LOCUS       DASWNS010000262         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_270081, whole genome shotgun sequence.
ACCESSION   DASWNS010000262 DASWNS010000000
VERSION     DASWNS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270081"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /locus_tag="VGB76_09315"
     CDS             complement(<1..1809)
                     /locus_tag="VGB76_09315"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099139.1"
                     /translation="MNTRSPQNGDQEPETSTVKAAGVVNLSDARRRVRERSAAARAAL
                     AAEVAATRELLDRGQATEAESRLLQIIKDARGDEVLLAQARCMLSVSLETQGRNADAL
                     AAVHMYEDPQARAGLDAEISVFLRIQLGLAYNYTGDHPKAIALLNATLREATDKASNA
                     QLGAIYVALARVYRYINEYPIARDNSQRALSHFRNTGDWRGMAEAYRGLALECLFEGA
                     YEESLENYEQALKLVGDHPAPFMLGRIYANMAGACWFLKRPHDGIRYLEKAIDYYERT
                     DHKANAVDGYNNLGINLILVGDWSRAETVLERALTLASEFDEPGAKAKVPMILDSLGE
                     LYMLRGDLLEAEGHLQRAVRLAHDHGNKWYEGQALRTLSRCHLAMQESERALEESQMA
                     LSLAERIGDRQAVCDSRLLLAEVYLRCNQAHECAFELQKVTEQTSDSAADLAVAGEVQ
                     RVHGLLALAESDATLAIHHFGRSVSIFSILGDRYRTALALYGLGSAYETVQPERSAEH
                     FSRALHTFSELGARLDIARTEEALATTEVAAPEQRHETSAFAQLLTLRLAEAVASREL
                     LLRELAAVLDQETEARRVLITEIDEEGTIKVVIAHGD"
     gene            2372..>2800
                     /locus_tag="VGB76_09320"
     CDS             2372..>2800
                     /locus_tag="VGB76_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099140.1"
                     /translation="MATLTAPPKRPLGPFKRWLFKGVEEIEGPHEREGQHHTHPWWKV
                     MCLTGVDYFSTLGYQPGIAFLAAGALSPIATLVLVLLTLFGALPMYNRVAAESPHGDG
                     SISMLENLLSRWKGKLFVLALLGFAATSFIITITLSAADAA"
BASE COUNT          534 a          795 c          889 g          582 t
ORIGIN      
        1 gtcgccatgc gctatgacga ccttaatcgt gccttcttca tcaatctccg tgatgagcac
       61 gcgacgcgct tccgtctcct gatcgagcac ggcagccagt tcgcgcagca acagttcgcg
      121 cgaggcgacg gcttcggcga gacgaagcgt taacagttgc gcgaaggccg aagtctcatg
      181 tctctgttcg ggcgcggcta cttcggtagt ggcgagagcc tcttccgtgc gcgcgatgtc
      241 gaggcgcgcg ccgagttcgc tgaacgtgtg cagggcgcgc gagaaatgtt cggcggagcg
      301 ttcgggttgg acggtttcgt aagcggagcc gagtccgtag agggcgagcg cggtgcggta
      361 gcggtcgccg agaatggaga agatcgagac gctgcgcccg aagtgatgaa tggcgagcgt
      421 ggcatcggac tcggcgagcg cgagcagccc gtggacgcgc tgcacttcgc ccgccacggc
      481 caggtcggcg gcggagtcgg aagtctgctc ggtaactttt tgtaactcga aggcgcactc
      541 gtgcgcttgg ttgcagcgga ggtagacctc cgcgagcagc aggcgcgaat cgcagacggc
      601 ctgacgatca ccgatgcgct cggcgagcga gagagccatc tggctctctt cgagcgcgcg
      661 ctcggattcc tgcatcgcca gatggcaacg cgacagcgtg cgcaaagcct gcccttcata
      721 ccatttgttg ccgtggtcgt gtgcgagccg gacggcgcgt tggagatgcc cctcggcttc
      781 gagcagatcg ccgcgcagca tgtagagttc gccgagcgag tcgaggatca tcggcacttt
      841 ggccttcgcg cccggctcgt cgaattcgga agcgagcgtg agcgcgcgct ctaagaccgt
      901 ctccgcgcgt gaccagtcgc cgacgaggat caggttgatg ccgagattgt tgtagccgtc
      961 aacggcgttg gccttgtgat cggtgcgctc gtaatagtca atcgcttttt cgagatagcg
     1021 gatgccgtcg tgcgggcgtt tgaggaacca gcacgcgccc gccatgttgg cgtaaatcct
     1081 gccgagcatg aaaggcgcgg gatgatcgcc gacgagtttc aaagcctgct cgtaattttc
     1141 cagcgactct tcgtaggcac cttcaaacag acattcgagc gcgaggccgc gataggcttc
     1201 ggccatcccg cgccaatccc ccgtattgcg aaaatggctg agcgcgcgct gcgagttgtc
     1261 gcgtgcgatg ggatactcgt tgatgtagcg atagacgcgt gcgagggcga cgtagatcgc
     1321 gcccaattgc gcgttcgaag ccttgtcggt cgcttcgcgc agggtcgcgt tcaagagggc
     1381 gatggctttg ggatgatcgc ccgtgtagtt ataggcgagt ccgagttgga tacggaggaa
     1441 gacggagatt tccgcgtcga gtccggcgcg cgcctgcggg tcttcgtaca tgtggacggc
     1501 ggcgagcgca tcggcgttgc gtccctgcgt ttcgagcgag acggagagca tgcagcgcgc
     1561 ctgcgcgagc aatacctcgt cgccgcgcgc gtctttgata atttggagaa ggcgcgactc
     1621 ggcttcggtg gcctgtccac ggtcgagcag ttcgcgcgtg gccgcgacct ctgcggcgag
     1681 cgcggcgcgt gcggctgcgc tacgctcgcg cacacgacgg cgcgcgtccg acaaattgac
     1741 gacgcctgcc gccttcactg tcgaagtttc cggctcctga tcgccgttct ggggtgagcg
     1801 ggtattcata tcttaagacg caagaggtgc gaggcagcaa caaaaaggcg ctctcttgca
     1861 aaaggttaaa cagagtcggg tggaaaggaa tctgtctcag gcttcgagct tactactcga
     1921 aaacagtcct aaagggttgt acattccaga atttaacaga gatataggag agtgacaagt
     1981 gacaactgac gaacggtagc cggaaagccg tgaatctcga atcgtaaact gcgaacagac
     2041 tcggaacagt ttttctctac atcacaattt acgattcgag atttatcgtc tgcacccgtc
     2101 gctcgtcacc tgtcactcgt cactgttttt ttgtggcaga ggctttgacg tttaagttaa
     2161 agtattgtat tgagcaatcc aacgttgagg gcgaacttta aacagcgcga gcgttttcgt
     2221 gtgtattgac aagcgataag aggcgcaggc agaagacctc tattgaatgt tgatacgcag
     2281 gcacgcgcgc gcccgtttgc agacgattgt tctatcgaat ccaatgagga cattggcggg
     2341 cacctcgctt gcctctgtat gggagttgct gatggctact ctgactgcgc caccgaaacg
     2401 acctctggga ccgtttaagc gttggttgtt caaaggcgtc gaagagatag aaggaccgca
     2461 cgagcgggaa gggcagcatc acacgcaccc gtggtggaag gtgatgtgcc tgacgggcgt
     2521 ggactacttc tccacactcg gttatcaacc gggcatcgcc tttctcgccg ccggtgctct
     2581 ctctcccatc gccacgctcg tgctcgtcct gctcacgctc ttcggcgcgc tcccgatgta
     2641 caaccgcgtc gccgccgaaa gcccgcacgg cgacggttcg atctcgatgt tggagaacct
     2701 gctctcgcgc tggaagggca aactcttcgt gctcgccctg ctcggtttcg ccgccacaag
     2761 tttcatcatc acgatcacgc tctccgccgc cgacgccgcc
//
LOCUS       DASWNS010000263         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_425808, whole genome shotgun sequence.
ACCESSION   DASWNS010000263 DASWNS010000000
VERSION     DASWNS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425808"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VGB76_09325"
     CDS             <1..584
                     /locus_tag="VGB76_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY0099141.1"
                     /translation="GAAVGSYGVTMTVRPHASPCLRCIFEETPPAASAETCDTAGVIM
                     PIISIVAAVQTTEALKLLTGNSDALHGALMQFDVWRNEWRRIKLGLPSPDCPTCAHAR
                     YETLEADAAELATVLCGRDAVQISPRRSTRVDLGALAERLRAAGEVQVNEYLLRLRAG
                     DFELTVFQDARAIIRGTDDMAAARSLYARYVGN"
     gene            770..>2460
                     /locus_tag="VGB76_09330"
     CDS             770..>2460
                     /locus_tag="VGB76_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY0099142.1"
                     /translation="MLNNTFRPRFISILSLLLLLVFAADARAGGPVVWETNSREELLR
                     GEARGVSVTDTGALMLAPRFAQLFDTEQAYIWSTAADERGNVYLGTGHDGRIFRVGAD
                     GKGALLYDAAELDVTALVFGRDGALYAGTSPDGKVYRISADGRAAEYFDPPDKYIWSL
                     AVLADGALAVGTGDNGKIYRVRANAAKPEESLLIDVNETHVISLAVDKQGQLIAGTDP
                     GGLVLRVSPEGKAFALFDSPLREVHALAVAADNSIYALALSDAASSSRGSSAPAATGA
                     GGGASGTVVTTSSVVVEEVGQPSPFTNAAQPAARSRNELANARSAVFRILPDGGTDVL
                     WSSGSVTAFAVAPAPQGGSVLIGTSDKGRIYSVTDDGRDTLLIQSSEDQISSFVVRGR
                     EVFAASSNQGKLFRFAAEPVSEGTYESPVRDAKVVAAWGRIWWRGQGAVELQTRTGNT
                     ERPDMTWSDWSAPYRDPAGAAVSSPRARFIQWRAVLRAPANGAGGTNAGGSRIEDVSV
                     AYLPRNVAPEILSITTPPTGVALLPAIQIQIDPNLEASGFDPSLIGPVPQVPPRRA"
BASE COUNT          416 a          788 c          812 g          444 t
ORIGIN      
        1 acggcgcggc ggtcggctcc tacggcgtga ccatgaccgt gcgcccgcac gcgagtccgt
       61 gcctgcgctg catcttcgag gagacgccgc ccgccgccag cgcggagact tgcgacacgg
      121 cgggcgtcat catgccgatc atctctatcg tcgccgccgt gcagacgacc gaggcgttga
      181 agttgctcac gggaaactcc gacgccctgc acggcgcgct gatgcagttc gacgtgtggc
      241 gcaacgagtg gcgacggatc aaacttgggc ttccgtctcc cgactgcccg acctgcgcgc
      301 acgcgcgcta cgaaacgctt gaggccgacg ctgccgaact cgccaccgtg ctctgcggtc
      361 gcgacgccgt gcaaatctcg ccgcgacgct cgacgcgcgt tgatctgggc gcgctcgccg
      421 agcggctgcg tgcggcgggc gaggtgcagg ttaacgaata tttgctgcgc ctgcgcgcgg
      481 gcgatttcga gttaaccgtc tttcaagacg cacgcgccat cattcgcggc acggacgaca
      541 tggcggcggc tcgctcgctc tacgcgcgct acgtcggcaa ctaatggaag ttcggaattc
      601 agacgagcgg cggaaggggt gatgtctatg aaatacagat cttcaattca cgtctccaat
      661 caaaaagtgc tactaacctt catagcttca tccgaattcc tgcaacgctt cttttcaggt
      721 tatcatcaga accagaggca attattccaa caccacagaa acttcattga tgctcaataa
      781 cacatttcgc ccacgcttca tctccattct ctcacttcta cttttgctcg tcttcgccgc
      841 cgatgcgcgt gcgggtggtc ccgtcgtctg ggagacgaat tcgcgcgagg aacttctgcg
      901 tggggaagcg cgcggcgtct ccgtcacgga cacgggcgcg ctcatgctcg cgccgcgctt
      961 cgcccaactg ttcgacacgg agcaggctta catctggtca accgcggcgg acgagcgcgg
     1021 caacgtctat ctcggcacgg gacacgacgg acgcatcttt cgcgtcggcg cggacggtaa
     1081 gggcgcgctg ttgtacgacg cggcggagtt ggacgtgacg gcgctcgtct tcgggcgcga
     1141 cggcgcgttg tacgcgggca cgtcgccgga cgggaaagtc tatcgcatca gtgctgacgg
     1201 gcgcgcggcg gaatatttcg acccgcccga caagtacatc tggtcgctcg ccgtgctcgc
     1261 ggatggcgcg ctcgccgtcg gcacgggcga caacggcaaa atctatcgcg tgcgcgcgaa
     1321 cgcggcgaag cccgaagagt cgctcttgat cgatgtcaac gaaacgcacg tgatctcgct
     1381 cgccgtggat aaacaggggc aactgatcgc gggcactgat ccgggcggac tggtcttgcg
     1441 cgtctcgccg gaaggaaagg cgttcgcgct cttcgattcg ccgctcaggg aagtgcacgc
     1501 gctggccgtg gcggcggaca actcgatcta cgcgctggcc ttgagcgatg ccgcgtcgag
     1561 ttcgcgcggc agcagtgcgc ccgctgcgac gggcgcgggc ggcggggcga gcggaaccgt
     1621 cgtcaccact tcgagcgtgg tggtcgaaga ggtcgggcag ccttcgccct tcaccaacgc
     1681 cgcgcagcct gccgcgcgca gccggaacga gttggcgaat gcgcgcagcg cggtctttcg
     1741 tatcctgccg gatggcggga cggacgtgtt gtggagttcg ggttcggtga cggcctttgc
     1801 cgtcgcgccc gcaccgcagg gcggcagcgt cctcatcggc acgagcgaca agggacgcat
     1861 ctattccgtg acggacgacg ggcgcgacac gctccttatc cagtcgagcg aagatcaaat
     1921 ttcttcgttc gtcgtgcgcg ggcgcgaggt gttcgccgct tcgagcaatc agggaaaact
     1981 gtttcgcttc gcagccgagc cggtgagtga aggcacttat gaatcgcctg tccgcgatgc
     2041 gaaagtggtg gccgcgtggg ggcgcatctg gtggcgcggt cagggcgcgg tcgagttgca
     2101 gacgcgcacg ggcaacaccg agcgacccga catgacctgg agcgattgga gcgcgcctta
     2161 ccgcgacccg gcgggcgcag ccgtctccag cccgcgtgcg cgttttatac agtggcgcgc
     2221 ggtgctgcgt gcgcccgcaa acggcgcggg gggcacaaac gcgggcggtt cgcgcatcga
     2281 agatgtgagc gtcgcttact tgccgcgcaa cgtcgcgccg gaaattcttt cgatcaccac
     2341 gccgccgacg ggcgtcgccc tgctgcccgc gattcaaatt cagattgacc cgaatctcga
     2401 agcctccggt ttcgatccgt cgctcatcgg tcccgtgccg caggtgccgc cgcgtcgcgc
//
LOCUS       DASWNS010000264        20208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_450766, whole genome shotgun sequence.
ACCESSION   DASWNS010000264 DASWNS010000000
VERSION     DASWNS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20208
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450766"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VGB76_09335"
     CDS             <1..547
                     /locus_tag="VGB76_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099143.1"
                     /translation="WDLPIFRTRTDIVGKVLGGWQLSGILTKHSGFPWTPLLSDCVTT
                     RGPSLCPARPVAYFGGAGTDTSNEAFLNGSNFAGGGTRYFSTASPGNALPGIGRNVFR
                     GPRYFNVDMSATKRFGLPGFFGEAANFEFKANFFNAFNILNLQSFGFNSPSTVITNPN
                     FGRAERALAGRVIEIQGRLVF"
     gene            complement(643..2043)
                     /locus_tag="VGB76_09340"
     CDS             complement(643..2043)
                     /locus_tag="VGB76_09340"
                     /inference="COORDINATES: protein motif:HMM:NF045090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099144.1"
                     /translation="MNLRRISQRLSSSPSPGGASTCALFMLLLAANSAAPCAWAQKSA
                     TRTVAAQQSAGRVPAATPRVDVSSLLREAAALLQAGRLDEAEPVVRRAVAAAPRDANA
                     HNLLGVILDQRGGRVAEAERAYREALRLHPQSTSARANLGVLLARNGRSLEAVEIFEA
                     VLRDVPGHPQATLNLGLALYHLKRFDEAAAALEAAAKLSPAAAEPFYYLGLIARERGR
                     AEEAADHWQRALARRPNFPEVNFMLAEELRQQRRYEGAAEFYERALAQDPSKLVYYVR
                     LGGVYMLLVRYERALEIFRRAAERFPDAPEAHYFVGIAARGNATYEVAAKALRHSLAL
                     RPDNPDALAQLGFILTEQSNFAEAEALLRRAVALDAAHFYAHYDLGRLLSKGQRYDEA
                     ITVLRKAATIRADDPGVHYSLFMAFSRLKRKAEADAELSTFKRLEEERKRRRREGLED
                     HADEVDNPPASVPPPT"
     gene            2425..4773
                     /gene="bglX"
                     /locus_tag="VGB76_09345"
     CDS             2425..4773
                     /gene="bglX"
                     /locus_tag="VGB76_09345"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HEY0099145.1"
                     /translation="MKSAKRNSLLTACLLIAALLPVVPSAPAQRAAKGASASASERRI
                     NALIARMTLAEKLGQLQMLDGEANGNFRPEHRELIRKGLLGSTLNVRGVARTNELQRI
                     ALQESRLKIPALFAFDVIHGYRTIFPIPLGEAASFDRAAAERSAQIAAAEAAAAGVRW
                     TFAPMVDIARDARWGRIAEGAGEDPYLGSELARARVLGFQGRDYSAPDRIVACAKHWV
                     AYGAAEAGRDYNTTDMSEGTLRELYFPPFKAAVDAGVGTFMSAFNDLNGVPTSANPFT
                     LTQVLRGEWKFDGVVVSDYESVKELMNHAFAADEKEAASHALNAGVDIEMVSRLYNAH
                     GAQLLRERKLDMATIDEAVRRVLRIKFRLGLFERPYADEAREREVIMRPEHLAAAREI
                     AARSLVLLKNEREALPLSKNLRSIAVVGPLADSPKDVLGSWTGDGRVEDAVTLLAGVR
                     AKVSPQTKVLYAKGVAIEGRGVTGNYDAPPPAATNAGGTNVAQASDAARNSTTPLLEN
                     SLGEAVRTARAADAVIIAVGESAEMSGEAASRASLDLPGRQLELIQAIHATGKPYVVV
                     LMNGRPLTINWLAENSPAILETWFAGTQAGHAIADVLFGDVNPGGKLPVTFPRSAGQS
                     PLYYNHKRTGRPPTDQKYTSKYLDVPWTPLYPFGYGLSYTQFRLSNLQLSAQRIRPGG
                     QLTASVEVENIGRRAGDEVVQLYIRDVASSVTRPVRELKGFERVTLNAGERRRVAFKL
                     TPAELGFYNRDLRFVVEPGAFKVFAGTSSADGLEASFNVVER"
     gene            4788..6149
                     /locus_tag="VGB76_09350"
     CDS             4788..6149
                     /locus_tag="VGB76_09350"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY0099146.1"
                     /translation="MLPTSLQRFASSYLKMRGAFVSLLCAGLLLSNFALTATAQRVRR
                     VPARASARLSAADEAFLEDMSRRAFRFFWEQADPQTGLVRDRARTDNSPLDENHRDVA
                     SIAATGFGLTALAIAAERGWLTPHEARERVRTTLRFFATRATHEHGWFYHWMDAKTGE
                     RRWKSEISSIDTALLLAGVLTVRQKFRADPEIVRLADAIYERVDFSWMLNGHPTLLSH
                     GWRPETGFIKYRWDAYSEHLILYLLAIGSPTHAITPRSWLAWQRERITYAGYTYITGG
                     PLFIHQYSHAWADFRGRHESHYPFTDYFANSVAATRAHRAFCLALSGEFPGYTENVWG
                     ITASDSEKGYLAWGGPPRDAAIDGTVVPSAAGGSLMLTPDIALPVLRTIRDRYGARVY
                     SMYGFTDAFNPNNDWVNPDVIGINLGIMLLSAENLRTGNVWRWFMRNREIPRAMQLVG
                     LRS"
     gene            complement(6214..7659)
                     /locus_tag="VGB76_09355"
     CDS             complement(6214..7659)
                     /locus_tag="VGB76_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0099147.1"
                     /translation="MKHNSPPARLRKLTLAFLVMTSTYTHAPAQTPAPRDFSRETARD
                     VRPWVRDGVIYEIFPRAFSPQGNFNGITAQLDRLKELGVNILWLMPIHPVGRERAKGS
                     IGSPYAVRDYYAINPDYGTKEDLKRLVAEAHRRGMKVIIDIVANHTAWDSVLMQTPAF
                     YTHDARTGKIIPPVADWADVADLNYDNAQLRTYMTDMLKFWLREYDLDGFRCDVAGMV
                     PTDFWERVRTELERVKPDIFMLAEWHEPELLVKAFDVDYSWPVHSALTEVLMGNAPAT
                     NLRAAWEEEQAKWPRNSLHLRFSDNHDERRAIARFGERAALAASAFVFTLDGVPMLYN
                     GMEVGDTTESGAPALFERLPVFWQIAERRPEFPRFYKQIIALRSKSAPLRRGSTEWLR
                     NSDESRIVTYARRSEAEELIVAINFSNRPFTGTVEAAGAGNFTDVTPDVQPPLPPDAP
                     APERKPRARTVGLPALALDAWGYRIFRRALK"
     gene            complement(7850..9310)
                     /locus_tag="VGB76_09360"
     CDS             complement(7850..9310)
                     /locus_tag="VGB76_09360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0099148.1"
                     /translation="MTQQKPRLNFWQIWNMSFGFLGIQFGWGLQLANMSGIYTYLGAS
                     PDDVPILWLAGPMTGLLVQPIVGSMSDRTWNRLGRRRPYFLVGAIFASISLFFMPDSS
                     VLWMAAGLLWILDASINVSMEPFRAFVADKLNVDQRTAGFVMQSFFIGIGATMANALP
                     YLFRQYGVTGTTPSGIPLTVQYSFKIGAVVFLLAVLWTVFTTKEYPPEDIEEFDRQRR
                     ATYGDATGLSKIFKIVAELLREISGAFTKMPQTMKQLAAVQTFTWLGLFCMWMFFGLT
                     TSYHVFGATGSADPRFALGQEWGGNAFAIYSIVCFAVAFLLPKLAAATSRKAVHAFAL
                     VCGGIGLLSVYFIKDQNVLLLTMIGVGIAWASILSMPYAILSGALPAARMGVYMGIFN
                     FFIVIPEIVASLFFGRIIRAIFGENSTIAPLYMVMAGGAFLLIAAALVTFVRDVADHV
                     PEAAVIRGDEHEPLIPVGSVQPVPSTGVIGDASRKA"
     gene            complement(9435..10163)
                     /locus_tag="VGB76_09365"
     CDS             complement(9435..10163)
                     /locus_tag="VGB76_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0099149.1"
                     /translation="MALRNKPLTEQVALITGGGTGIGRAFAAVLAESGARAVVIASRR
                     EEVLRRTAEELNAQAGAERVYPYAFDIRDLAQTETLVRYVAERFGTIDLLVNNSGLAV
                     PETVEAITEEGWNKVLETNLRGAMWLVRAALPYMIREDFGDVVNISSQAGKQGYADVP
                     SYCASKFGLLGYAESLRDHARKTGANLRVFNFCPGLVDVEHTGADAEPRAGAIHVRNM
                     ARTLLFALSLDRNVVVEEISLYAR"
     gene            complement(10213..12135)
                     /locus_tag="VGB76_09370"
     CDS             complement(10213..12135)
                     /locus_tag="VGB76_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0099150.1"
                     /translation="MNISRRESFKRFALLLVLSLSTVSYVCGQSAPEVSKVEPPSWWT
                     QSTLNPVRLMLRGRNLTGARVEVVGAAGLQLGLTRINAAGTYLFVDVAIDPQAVPGMR
                     RLRITTPSGVGEAQFEVLAPLNRVGRFQGFSPDDLIYMLMIDRFSDGDATNNDPARSR
                     GIYDRNNKFYYHGGDLQGVINRLPYLKDLGVTSVWLSPWYDNYDRLNEIELKEGKPST
                     GFHGYNAQDFYAVEEHFGTLAKLRELVEEAHKSGIKIIQDSVVNHTGPYHPWVADQPT
                     PTWFNGTRERHLANVFETWVLHDPRPDPQIKRATLEGWFLDFLPDFNQSDEETRRYLI
                     QNTLWWIGVTGLDAIREDTWQYVPNDFWREWMAAVKREFPQVRVFGEVKDSDITHVSF
                     FQGGRVRFDGVDTGLDSLLDFPVFYHARSTFGLGRPIRDVAQILARDYLYTNPDLLVT
                     LVGSHDDGRFMSERGATVAGLKLAHTFLLTTRGVPQIYYGDEIAMTGADEPTTRQDFP
                     GGFPGDRRNAFARGGRTPEEQDIFEHTRRVARLRAELEPLRRGTLVTLHAARQQYAYA
                     RVAEGTRGGAVIVVLNNDTKPATVEFDVAPARLPDDVSLDDRLDTLRNVRVQGGKLKL
                     TLPTRSATILTNIPAR"
     gene            complement(12257..13819)
                     /gene="malQ"
                     /locus_tag="VGB76_09375"
     CDS             complement(12257..13819)
                     /gene="malQ"
                     /locus_tag="VGB76_09375"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY0099151.1"
                     /translation="MSFTRSSGILLHPTSLPGSYGVGDLGSAAYAFIDFLAASGQSLW
                     QVLPLGPTGYGDSPYQSFSAFAGNTLLVSPERLVTENLLDQRDLAGAPEFADERVDFG
                     AAIEFKNRLLRRAFENFRHTTNTGLRGSFETFSQHEALWLDDYALYRAMKDARGGVAW
                     NKWERPMLKREPHALAAAREGLREQIDAHKFYQFLFFRQWHALKRYANERGISIVGDI
                     PIFVAHDSADVWTNPHLFKLNEDGSPRVVAGVPPDYFSATGQLWGNPLYDWARLQAED
                     FRWWIARVRSMLHTVDILRIDHFRGFAASWEIPGGDQTAERGHWVNAPGRELFTAIKS
                     ALGDAPILAEDLGVITPDVEALRDDFNFPGMRILQFGFGGDSKNIDLPHNYHRNVAVY
                     TGTHDNDTTVGWFESAAGAGSTRKPEQIERERKFCLDYLNSDGEEIHWDFIRAVLASV
                     GNIAIIPLQDVLGLGNEARMNLPNSTSGNWSWRYTTGALTNNLRDRLKDLTALYGRNL
                     KTEVSDLKSDEH"
     gene            complement(14036..15346)
                     /locus_tag="VGB76_09380"
     CDS             complement(14036..15346)
                     /locus_tag="VGB76_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0099152.1"
                     /translation="MLKPGAFALFLILALLCTPLSSASQQKKNVDPNRQKEPAVSAPA
                     KIEDAPVVEPQEDGEVVKVDTSLVSIPVVVSDRAGVYVPDMSKEEFTLTEDGVRQELA
                     FFSTVSQPFTVILMLDTSASTEEKLRDIRRAANAFVSGLQPQDRIKVMSFDDEVRDLN
                     PFTSDHAIVERAIQATRAGKGTKLYDAMQTALSLLKPDKARRKAVVIFTDGVDYRSDS
                     FRYINNIRALEESGVIVYPIRYDTRIETERIAREQAAGGGRPDLAAIIKGRGDGRSLP
                     IPTGTTFPGGTIPSGRGDGQPTIGGIPIPTIPAPPRRDDDPRQTRRDDPRLPRSPGEV
                     SAPGTSHEDGIGMMLNSLYKTADSYLNELAQKSGGKLTRADTLERLPAAFKEIAAELR
                     TQYAIGYYPSNPVKNGSYRKIKITSTRKGANVRARPGYSAVSVP"
     gene            complement(15525..17438)
                     /locus_tag="VGB76_09385"
     CDS             complement(15525..17438)
                     /locus_tag="VGB76_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0099153.1"
                     /translation="MPAKHRARSSLLAIFALVCLAASAHAQNSTAPVVAKVEPPSWWA
                     AHTINPVRLLVRGTNLHGARVVSARRELEPGGAVVNAAGTYVFVSVRISPDAAPGDYP
                     LTLETRGGKTSIPFRLNVPLDPATHFQGITTDDVIYLIMPDRFANGDAANDIPPGAPK
                     EATDRRNPRAFHGGDLRGIINRLPYLKELGVTALWLNPWYDNWNGVKTCAQPWCPNTY
                     YHGYHAIDYYAVEDRFGDMATLRELVEKAHAAGIKIIQDQVANHVGSQHPWVNDPPLD
                     NWFHGTLARHTQNPFRGDLLLSPHASEAARRPTLDGWFSDDLPDMNQEEPEVARYEIQ
                     NALWWVGITGMDGIRQDTIQYMPRAFIRELSLALRRQYPKMWMVGEVFDRDPVHLSYF
                     FGGRAGWDGIDTELDSLFDFPLWQTSLDAFTNKAPVNALRSMLKNDALYEDAARLVVM
                     TNNHDVRRIASVEGMTLEGSMLHHAFLFSVRGIPQLYYGDEIFMEGGDDPDNRRDFPG
                     GWSGDARNAFERRGRTAREARMFEWTRDWLKLRREHAAMRHGRLLDLAFDENSYAFAR
                     RTANETIILAFNRAAAAKRISAPAAFVEVADGAELVPLLVAKERVRIEGGEITFEVPA
                     RTAVAYLVAAKAR"
     gene            complement(17558..17968)
                     /locus_tag="VGB76_09390"
     CDS             complement(17558..17968)
                     /locus_tag="VGB76_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0099154.1"
                     /translation="MRRRGKLIIVALSLAGLFLSGSYSGRRARAEEGARAAAAAQKKL
                     SRKQQLANARVLYMENCARCHGADGRGQTPMGKVFSVANLADAKWWKKERVGDKRLTA
                     AIRDGRNQMPAFGKKLSKEEIAALVVFVKTFNGK"
     gene            complement(18213..20015)
                     /locus_tag="VGB76_09395"
     CDS             complement(18213..20015)
                     /locus_tag="VGB76_09395"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099155.1"
                     /translation="MLYQSPTRRSPDSLLQSAARLLACLTLTGIYAAPAPAQRRRAPT
                     AARPAAATQQGTAKGTAGRPTSPVAARGQADAATASLLREAAALLQSGKLVEAEAAAR
                     KVLNVAPRDPNARTLLGVVLDKSGRAVEAEREYREAIKSAPQHAPAHANLGVLLSRTN
                     RVEQAIESFEAALRVAPENAFAVFNLGALYAARGDYKRAIPLLERAAGINPASPAASS
                     NDLALLLTLTNAYAHDGRSDDAGRLAERIERLANDDPRVLFTLALSLAEAHEYARAVP
                     LFERTNALRPQTFEVLYNLGVALYNLDRLDEATRALEAAAALAPNEAEPYYRLGLVAA
                     ARNDGQAAVELWRKALALRPAFPEANFMIGEELLKRRATDEAIPFYQRALEQDSTKLI
                     YHIRLGVANFRSKHYPRAREVFANALKRFPANANLYYLIGYAARAEGQYDDALAAFRQ
                     SLALQPDNPDVLTNLGYIANQRGQTEESERLLRQAIALDPQNFAAHHDLGRLLVKLKR
                     YEEALPPLERGAALNANDPGIHYQLFTAYSRLKRKSDADRELAIFKRFQEARKHDPTP
                     LGGEAATGATDEGDKPAPAPLPASVSGEGVKPPR"
BASE COUNT         3626 a         6178 c         6596 g         3808 t
ORIGIN      
        1 ctgggatttg ccgatcttcc gcactcgcac agacatcgtc ggcaaggtgc tcggcggctg
       61 gcagttgagc ggcatcctca cgaagcattc gggctttccc tggacgccgc tgttgtctga
      121 ctgcgttacc acgcgcggcc cgagcctctg ccccgcgcgc cccgtcgcct acttcggcgg
      181 cgcgggcacg gacacttcca acgaagcctt cctcaacggc agcaacttcg ccggtggcgg
      241 gactcgatat ttcagcaccg cctcgcccgg aaacgccttg cccggcatcg ggcgcaacgt
      301 ctttcgtggg ccgcgctact tcaatgttga catgagcgcg accaaacggt tcgggctgcc
      361 cggcttcttc ggcgaagcgg ccaacttcga gtttaaggca aacttcttca acgccttcaa
      421 catcctgaac cttcaatcgt tcggcttcaa cagcccgagc acggtgatca ccaatcccaa
      481 cttcggtcgc gccgagcgcg cgctcgccgg tcgcgtcatc gagatacagg ggcggctcgt
      541 cttctagttt tacttcccac taactcgcgc gggtggatga actgaacaat tcaattcatc
      601 cacccgcgtc tctcttttcg ctgccggaag caatttagaa gttcatgtcg gcggcgggac
      661 ggaagcgggc gggttatcaa cttcgtctgc gtgatcttcc agcccttccc tgcggcgacg
      721 cttgcgctcc tcttcgagtc gcttgaatgt gctcaactcg gcatcggctt ccgctttgcg
      781 cttgagccgc gaaaaggcca taaacaggct gtaatggacg ccgggatcgt cggccctgat
      841 agtcgccgct tttcgcagca ccgtgatcgc ttcgtcgtaa cgctgacctt ttgagagcag
      901 gcgaccgaga tcgtagtggg cgtaaaaatg ggcggcgtcg agcgcgacgg cgcggcgcag
      961 cagtgcttcc gcctccgcaa agttgctctg ttcggtgagg atgaagccta gttgcgcgag
     1021 ggcgtcgggg ttgtcgggtc tgagcgcgag cgaatggcgc agagcctttg cggccacctc
     1081 gtaggtggcg ttgccgcgcg cggcgatgcc gacaaaatag tgtgcctccg gcgcgtctgg
     1141 aaaacgctct gcggcgcgtc ggaaaatttc gagcgcgcgt tcgtagcgaa ccagcagcat
     1201 gtagacgccg ccgagccgca cgtaatagac cagcttagac gggtcttgcg cgagcgcgcg
     1261 ttcgtaaaac tcggctgcgc cttcatagcg acgctgctgc cggagttctt cggcgagcat
     1321 gaaattaact tcgggaaagt tcggtcgtcg cgcgagcgcg cgttgccagt ggtctgcggc
     1381 ctcttccgca cgcccgcgct ctcgtgcgat aaggccgaga tagtagaacg gttcggcggc
     1441 agcgggtgag agtttcgcgg cggcttcgag tgcggcggcg gcttcgtcga aacgcttgag
     1501 atgatagagc gcgaggccga gattcagagt ggcctgcggg tgccccggca catctctgag
     1561 caccgcctcg aaaatctcga ccgcttcgag cgagcgaccg ttgcgcgcca gaaggacgcc
     1621 gagattcgcc cgcgccgaag tggactgcgg atgcagacgc aaagcctcgc ggtaggcgcg
     1681 ttcggcctcg gccacgcgcc cgccgcgctg gtctaagatc acgccgagca aattgtgcgc
     1741 gttcgcatct cgcggggcag cggcgacggc gcgacgcacg acgggttcgg cctcgtcgag
     1801 tctgcccgcc tgcaacaacg ccgccgcttc cctgagcaac gacgaaacat ctacgcgcgg
     1861 cgtcgccgcc ggaacgcgtc cggcagactg ctgcgccgcg accgtacggg tcgcactctt
     1921 ctgcgcccac gcgcaaggcg cggcagagtt cgcggccagc agcaacatga acagggcgca
     1981 cgtggacgcg ccaccggggg acggcgacga cgacaggcgt tgagagatgc ggcggagatt
     2041 catgacaaca ctgcgagcaa ggcgtccggc ctttgcgcct tcctttgcgc cttctcttgc
     2101 accttccttt gcgtctttgc ctctccggcg cgaactctat ttctcgccgg aggcgcagag
     2161 ttaagacgac aggcgcaagg gaagcgtaag gagtagtctt gaagtcgtgc gctagggatc
     2221 ggggcggcgt atgtcgctgg caaagagcgt tgtagtatag tcaacgcaaa gattttgcgc
     2281 caaacttccc ttaacgatag tcgtcattta atctccggca acatgaagat gccggttcgc
     2341 atgtccgccg ctcggttcat aaatttctgg cgcgcgtact caatccagta gctttaaact
     2401 tcgcagcctc gaaggagatg ttcgatgaag tctgccaaac gaaacagtct gctaacggcc
     2461 tgcctgctca tcgctgcgtt gctgcccgtc gtcccttccg ccccggcgca acgtgcggct
     2521 aagggggcgt ccgcgagcgc gtctgaaaga agaatcaacg cgctcatcgc gcgcatgacg
     2581 ctcgccgaaa aactcggcca gctacagatg ctcgacggcg aggccaacgg caacttccgc
     2641 cccgaacacc gcgagttgat ccgcaaaggc ttgctcggct ccacgctcaa cgtgcggggc
     2701 gtggcgcgca cgaacgagtt gcagcgcatc gccttgcaag agtcgcgctt gaagattccc
     2761 gcgctctttg ccttcgacgt gattcacggt taccgcacca tcttcccgat cccgctcggc
     2821 gaggccgcga gtttcgaccg ggctgcggcg gaacgctccg cgcagatcgc tgcggcggaa
     2881 gcggcggcgg cgggcgtgcg ctggaccttc gcgccgatgg tggacatcgc tcgtgatgcg
     2941 cgttgggggc gcatcgcgga aggcgcgggc gaagacccgt atctcggctc ggagttggcg
     3001 cgtgcgcgcg tgctcggctt tcaggggcgc gactacagcg cgcccgaccg catcgtcgcc
     3061 tgcgccaagc attgggtggc ctacggcgcg gcggaggcgg ggcgcgatta caacacgaca
     3121 gacatgtccg aagggacgct gcgcgaactc tatttcccgc ccttcaaggc ggcggttgac
     3181 gccggagtcg ggacgtttat gagcgcgttc aacgatctca acggcgtgcc gacttcggcc
     3241 aacccgttca cgctcacgca agtcctgcgc ggcgagtgga agttcgacgg cgtggtcgtg
     3301 agcgattacg agtccgtcaa ggaactgatg aaccacgcgt tcgcggcgga tgaaaaagag
     3361 gccgcgagcc atgcgctgaa cgcgggcgtt gacatcgaga tggtttcccg cctctacaac
     3421 gcgcacggcg cgcaactgct cagggaacgc aagctcgaca tggcgacgat tgacgaggcg
     3481 gtgcgtcgcg tcctgcgcat caagttccgg ctcggcctct tcgaacgtcc ctacgcggac
     3541 gaggcgcgcg agcgcgaagt gatcatgcga ccggaacatc tcgccgccgc gcgcgagatc
     3601 gccgcacgct ccctcgtgct cttgaagaac gagcgcgagg ctctgcccct gtcgaagaat
     3661 ttgagatcaa tcgccgtcgt cggacctctc gccgattcgc cgaaggacgt gctcggctcg
     3721 tggacgggcg acgggcgcgt ggaagatgct gtcacgctgc tggcgggcgt gcgtgcgaaa
     3781 gtctcgccgc agacgaaagt tttatatgcg aagggcgtgg cgattgaagg ccggggcgtg
     3841 acgggcaact acgacgcgcc gccgcccgcc gccacgaacg ccgggggaac caacgtggcg
     3901 caggcgagcg acgccgccag aaactcgacc acgccgctgc tggagaacag cttgggcgag
     3961 gcggtgcgca cggcgcgtgc ggctgatgcg gtcatcatcg ccgtcggcga atcggcggag
     4021 atgagcggcg aggctgcatc acgcgcatcg ctcgatctgc ccggtcggca gttggagttg
     4081 attcaggcca tccacgcgac gggcaaacct tacgtcgtgg tgctgatgaa cggtcgcccg
     4141 ctcacgatca actggctcgc ggaaaactcg cccgcgatcc tcgaaacgtg gttcgcaggg
     4201 acgcaggccg ggcacgccat cgccgacgtg ctcttcggcg acgtaaatcc cggcggcaaa
     4261 ctccccgtca ccttcccgcg ctccgcaggg caatcgccgc tctactacaa tcacaagcgc
     4321 acgggtcgcc cgccgaccga tcagaaatac acctcgaaat atctggacgt gccctggacg
     4381 ccgctctacc cgttcggcta cgggttgagc tacacgcagt ttcgcctgtc gaatctgcaa
     4441 ctgagcgcgc agcgcattcg ccccggcgga caactgacgg cgagcgtcga agtggagaac
     4501 ataggacggc gcgcgggcga tgaagtcgtg cagctttaca tacgcgatgt cgcatcgagc
     4561 gtgacgcgcc cggtgcgtga gttgaaaggc ttcgagcgtg tgacgctgaa cgcgggcgag
     4621 cgtcggcgcg tcgccttcaa gctcacgccc gccgaactcg gcttctataa ccgcgacctg
     4681 cgtttcgtcg tcgaacccgg cgcattcaaa gtcttcgcgg gcacgagttc cgccgatgga
     4741 ctcgaagcga gcttcaacgt tgtcgagagg taagcgagaa gtagccgatg cttccaacat
     4801 ctcttcaacg cttcgccagc agttatctca agatgcgcgg cgcgttcgtc tcgcttctct
     4861 gcgccgggct gcttctctcc aactttgcgc tcacggcaac ggcgcaacgc gtgcgccgcg
     4921 ttccggcgag agcgtccgcg cgcctctccg cagcggatga agcgtttctc gaagacatgt
     4981 cgcgccgcgc gtttcgtttc ttttgggagc aggccgaccc gcagaccgga ctcgtccgcg
     5041 accgcgcacg cacggacaac tcgccgctcg acgaaaacca ccgcgacgtg gcgagcatcg
     5101 ccgccacggg cttcggcctc accgcgctcg ccatcgccgc cgaacggggc tggctcacgc
     5161 cgcacgaggc gcgcgaacgc gtacgcacga cgctccgctt cttcgccacc cgcgccacgc
     5221 acgagcacgg ctggttctat cactggatgg acgcgaagac gggcgagcgc aggtggaaga
     5281 gcgaaatctc ctcgattgac accgcgctcc tgctcgcggg cgtactgacg gtgcgccaaa
     5341 aattccgcgc cgacccggag atcgtgcgcc tcgccgacgc catctacgag cgcgtcgatt
     5401 tttcgtggat gctcaacggc cacccgacgc tgctctcgca cgggtggcga cccgaaaccg
     5461 gcttcatcaa gtatcgctgg gacgcctaca gcgaacattt gatcctctac ctgctcgcca
     5521 tcggttcgcc cacgcacgcc atcacgcccc gctcctggct cgcatggcag cgcgagcgca
     5581 tcacctacgc gggctacacc tacatcaccg gcggacctct cttcatacat caatactcgc
     5641 acgcgtgggc agactttcgc gggcggcacg aaagccacta tcccttcaca gactacttcg
     5701 ccaactccgt cgcggcgacg cgcgcacacc gcgctttctg tctcgctctc tccggcgagt
     5761 ttcccggtta cacggagaac gtgtggggca tcacggcttc ggatagcgag aagggctatc
     5821 tggcatgggg cggtccaccg cgcgacgcgg cgattgacgg cacggtcgtc ccatctgcgg
     5881 cgggcgggtc gctcatgctc acgcccgaca tcgcgctgcc cgtgctccgc accatccgag
     5941 acaggtacgg cgcgcgcgtc tacagcatgt acggcttcac agacgccttc aatcccaaca
     6001 acgattgggt caacccagac gtgatcggca tcaatctcgg catcatgctg ctcagcgcgg
     6061 agaacctgcg cacgggcaac gtctggcgtt ggttcatgcg caacagggag ataccgcgcg
     6121 cgatgcaact cgtcgggctt cgctcttgag acaaagaaag gccggtgggg tttataagcc
     6181 accggcctga aactgcggtc agaatatttc ctactatttg agcgcgcggc gaaagatgcg
     6241 atagccccat gcgtcgagcg cgagcgcggg gaggccgacg gtgcgtgcgc gcggtttgcg
     6301 ttcgggggcg ggcgcatcgg gcggcagcgg cggctgcacg tccggcgtta cgtccgtgaa
     6361 gtttcccgcg cccgccgctt cgaccgtgcc cgtgaacgga cggttcgaga agttgatcgc
     6421 gacgatcaac tcttccgcct cggagcgacg cgcgtaagtg acgatgcgcg actcgtcgga
     6481 gtttcgcagc cattcggtcg aaccgcgcct gaggggcgcg ctcttgctgc gcagcgcgat
     6541 gatctgtttg tagaagcgcg gaaactccgg tcgccgctcg gcgatctgcc agaagacggg
     6601 aagccgctcg aagagcgcgg gcgcgcccga ctccgtcgtg tcgcccactt ccatgccgtt
     6661 gtagagcatc ggcacgccgt cgagggtgaa gacgaaggcg gaggcggcga gggcggcgcg
     6721 ttcgccgaaa cgcgcgatgg ctcgtcgctc gtcgtgatta tcggagaagc gcaggtgcag
     6781 cgaattgcgc ggccacttcg cttgctcctc ttcccatgcg gcgcgcaggt ttgtcgccgg
     6841 agcgttgccc atcaacacct ccgtcaacgc gctatgaacc ggccaagagt agtcaacgtc
     6901 gaaggctttc accagcaact ccggctcgtg ccattcggcg agcatgaaga tgtccggctt
     6961 gacgcgctca agttccgtgc gcacgcgctc ccagaaatcg gtcggcacca tgcccgccac
     7021 gtcgcagcga aaaccgtcga ggtcgtattc gcgcagccag aacttgagca tgtccgtcat
     7081 gtaggtgcga agctgtgcgt tgtcgtagtt gaggtcggcc acgtccgccc aatcggcgac
     7141 gggcgggatg atcttgcccg tccgtgcgtc gtgcgtgtag aaggcgggcg tctgcatcag
     7201 cacgctgtcc caggcggtgt ggttcgccac gatgtcgatg attactttca tcccgcgccg
     7261 gtgcgcttcc gcgacgagcc gcttgaggtc ttctttcgtg ccgtagtccg ggttgatggc
     7321 gtagtagtcg cgcacggcgt aggggctgcc gatggagcct ttcgcgcgtt cgcgcccgac
     7381 cggatgaatc ggcatgagcc acaggatgtt gacgccgagt tctttcagac gatcaagctg
     7441 cgcggtgatg ccgttaaagt ttccctgcgg cgagaaggcg cgcgggaaga tttcatagat
     7501 cacgccgtcg cgcacccacg gtcgcacgtc gcgcgccgtc tctctggaga agtcgcgcgg
     7561 cgcgggagtt tgcgcgggcg cgtgtgtgta agttgaagtc atgacaagaa aggccaacgt
     7621 cagtttgcgt aacctcgcgg gcggcgaatt gtgcttcatg gtttgcgttg ctttctctcg
     7681 gcgtaaacgg ctctgtagaa tccctgttcc gagacgatca actaactgca cccgcgcgct
     7741 tccgaagcga agcacagcgg cgcgactaac gccagcaacg cgacggcgac tctgcgccgc
     7801 caaagcctgc gccgcgcacc cggccacctg aaaaaagcgt ctaacatcct taagcctttc
     7861 gcgacgcgtc gccgatcacg cctgtgctcg gcacgggttg cacggaaccg accgggatga
     7921 gcggttcgtg ttcgtcgccc ctgatgaccg ccgcttcggg tacgtggtcg gccacgtcgc
     7981 gcacaaacgt gacgagcgcc gccgcgatca gcagaaacgc gccgcccgcc atcaccatgt
     8041 agagcggcgc aatggtgctg ttctcgccga agatggcgcg gatgatgcgc ccgaagaaga
     8101 gcgaggcgac gatctccgga atcacgatga agaagttgaa gatgcccatg tagacgccca
     8161 tgcgcgcggc gggcagcgcg cccgacagga tcgcgtaagg catcgagagg atcgaagccc
     8221 atgcgatgcc gacgccgatc atcgtcagca gcagcacgtt ctgatctttg atgaaataga
     8281 ccgagagcag cccgatgccg ccgcagacga gcgcgaaggc gtgcaccgct ttgcgactcg
     8341 tggcggcggc cagtttcggc aacaaaaagg cgacggcgaa acagacgatg gaatagatgg
     8401 cgaaggcgtt gccgccccac tcctgaccga gtgcgaagcg cgggtcggcg ctgcccgtcg
     8461 cgccgaagac gtgataagag gtggtcaggc cgaagaacat ccacatgcag aagaggccga
     8521 gccacgtgaa ggtctgcacg gcggcgagtt gcttcatcgt ctgcggcatc ttggtgaacg
     8581 cgccggaaat ctcgcgcagc agttcggcca cgatcttgaa tatcttcgag aggccggtcg
     8641 cgtcgccgta agtcgcacgc cgttggcggt cgaactcctc gatgtcctcc ggcggatact
     8701 ccttcgtcgt aaagaccgtc cacagcactg cgagcagaaa gaccaccgcg ccgatcttga
     8761 aagagtattg cacggtgaga gggatgccgc tcggcgtcgt gcccgtcacg ccgtactgcc
     8821 ggaagagata gggcaaggcg ttagccatcg tcgcgccgat gccgatgaag aacgactgca
     8881 tgacgaaccc tgcggtgcgc tggtctacgt tcagcttgtc tgcgacgaag gcgcgaaacg
     8941 gttccatcga gacattgatg ctggcgtcga gaatccagag cagccccgcc gccatccaga
     9001 gcacggagga atcgggcatg aagaagagcg agatgctggc gaagatcgcg ccgacgagaa
     9061 agtaagggcg gcgacgaccg aggcgattcc acgttcggtc gctcatcgag ccgacgatgg
     9121 gctgcacgag gagtcccgtc attggtcccg ccagccagag gatcggcacg tcgtctggac
     9181 tcgcgccgag atacgtgtag atgccgctca tgttggcgag ttgcaagccc caaccgaact
     9241 ggataccgag gaatccgaaa ctcatgttcc agatttgcca gaagttcaaa cgtggctttt
     9301 gctgcgtcat ctctctctcc tttaaaagct attagctgtt ggctctcagc tttaaaatct
     9361 ttccggggcg ggcaactttc acctgcacga gcaaccgcag agaacgcttt ttgtactctg
     9421 ccgaccgcta accgctatct cgcatacagg ctgatctctt cgacgacgac gttgcggtcg
     9481 agcgagagcg cgaacaggag cgtgcgcgcc atgttgcgga cgtggatcgc gcccgcgcgt
     9541 ggttcggcgt ccgcgcccgt gtgttcaacg tctacgaggc cggggcagaa gttgaagacg
     9601 cgcaggttcg cgccggtctt gcgcgcgtgg tcgcgcagag attccgcgta gccgagcagg
     9661 ccgaacttcg aggcgcaata ggaaggcacg tctgcgtagc cctgcttgcc cgcctgcgaa
     9721 gagatgttaa ccacgtcgcc gaagtcttcg cggatcatgt agggcaaggc ggcgcgcacg
     9781 agccacatcg cgccgcgcaa gttagtctcc agaactttat tccaaccctc ttccgtgatc
     9841 gcttcgaccg tctccggcac ggccaacccc gaattgttga cgagcaggtc aatcgtgccg
     9901 aagcgttcgg cgacgtagcg gacgagcgtt tcggtctgtg cgagatcgcg tatgtcgaag
     9961 gcgtaaggat acacgcgctc tgcgcccgcc tgcgcgttca actcctccgc cgtccgcctg
    10021 agcacctctt cgcggcgcga tgcgataacg acggcgcgcg cgcccgactc ggccaacacg
    10081 gcggcgaacg cgcgcccgat cccggttccg ccgcccgtga tgagggcgac ttgttccgtc
    10141 agaggtttat tgcgtagagc catcgtttac ccgtctcttt cgagcgtgcg cagcagcggc
    10201 gctttcgtca cgttaacgcg ccggaatgtt cgtcaggatg gtggccgaac gcgtgggcag
    10261 cgtgagtttc agtttgccgc cctgaacgcg cacgttcctc agcgtgtcga gacgatcatc
    10321 gagcgacaca tcatccggca gacgcgcggg cgcaacgtcg aactctacgg tcgcgggctt
    10381 cgtgtcgttg ttcaggacga cgatgaccgc accacccctc gtgccttcgg cgacgcgcgc
    10441 gtaagcatac tgctgccggg cggcgtgcag tgtgacgagc gtgccgcgtc ggagcggttc
    10501 gagttccgcg cgcaggcgcg ccacgcgccg cgtgtgctcg aagatgtcct gctcttccgg
    10561 tgtgcgcccg ccgcgcgcga aagcgttgcg acggtcgccg gggaacccgc cggggaaatc
    10621 ctgccgcgtc gtcggctcat ccgcgcccgt catcgcgatt tcgtcgccgt aataaatctg
    10681 cggcacgccg cgcgtggtca agagaaaggt gtgcgccagt ttcagaccgg cgacggtcgc
    10741 tccccgctcg ctcataaaac gcccgtcgtc gtggctgccc acgagcgtca cgagcaggtc
    10801 ggggttcgtg tagagataat ctctggcgag tatttgcgcc acgtctctga tgggtctgcc
    10861 gaggccgaag gtggagcgcg cgtgatagaa gaccgggaag tcgagcagcg aatcgaggcc
    10921 ggtatccacg ccgtcgaaac gcacgcgccc gccctgaaag aaggagacgt gtgtgatatc
    10981 gctgtcctta acttctccga agacgcgcac ctgcggaaac tcgcgcttga ccgccgccat
    11041 ccactcgcgc cagaaatcgt tcgggacgta ttgccaggtg tcttcgcgga tggcgtcgag
    11101 gccggtcacg ccgatccacc agagcgtgtt ctggatgaga taacggcgcg tctcttcgtc
    11161 gctctgattg aagtcgggca gaaagtcgag gaaccagcct tccagcgtcg cgcgcttgat
    11221 ctgcgggtcg gggcgcgggt cgtgcagcac ccacgtctcg aagacgttgg cgagatgacg
    11281 ctcgcgcgtg ccgttgaacc acgtcggcgt cggctggtca gcgacccaag ggtgatatgg
    11341 tcccgtatga ttgacgacgc tgtcctgaat gatcttgatg ccggatttgt gcgcctcttc
    11401 gacaagttcg cgcagtttgg cgagcgtgcc gaagtgctcc tcgacggcgt agaagtcctg
    11461 cgcgttgtag ccgtgaaaac ccgtgctcgg cttcccttcc ttcaactcga tctcgttcaa
    11521 gcgatcatag ttgtcgtacc acgggctgag ccagacggac gtgacgccga ggtctttgag
    11581 gtaaggcagg cgattgatga cgccctgcaa gtcgccgcca tgatagtaga acttgttgtt
    11641 gcgatcatag atgccgcgcg accgtgcggg gtcgttgttg gtcgcgtcgc cgtcgctgaa
    11701 acggtcgatc atcagcatgt agatcagatc gtcgggcgag aagccttgaa agcgtcccac
    11761 gcgattcagc ggcgcgagca cctcgaactg cgcttcgccc acgccggagg gggtcgtgat
    11821 gcgcaggcga cgcatgcccg gcacggcctg cggatcaatc gccacatcca cgaagagata
    11881 ggtgcccgcc gcgttgatcc gcgtgagtcc caactgcaat cccgccgcgc cgacgacttc
    11941 gacgcgtgcg cccgtcaaat ttcgtccccg cagcatgagg cgcaccgggt tcaaagtgct
    12001 ctgcgtccac caactcggcg gctcgacctt tgatacctcc ggcgcgctct gcccacagac
    12061 gtaggagacg gttgagaggg agaggacgag caggagggca aaacgtttga agctctctcg
    12121 acgtgaaatg ttcatgaaat ttatgcgcct gttatttgcc ggtgcgtagc acgggataga
    12181 gggtgaaagt tgaaagcagg gatgcgccgg agtgaatgat ctcttgcgtt cgtcatctcc
    12241 cggctctccc ctttcttcaa tgctcgtcag acttcagatc gctgacttcg gttttcaggt
    12301 tgcgcccgta gagcgcggtc aagtctttga ggcggtcacg caggttgttt gtgagcgcgc
    12361 ccgtggtgta acgccaagac cagttgccgg aggtgctgtt ggggagattc atgcgcgctt
    12421 cattgccgag gccgagcacg tcctgcaaag ggatgatggc gatgttgccg accgaggcga
    12481 ggacggcgcg gatgaaatcc cagtgaatct cttcgccgtc ggagttgaga tagtcgagac
    12541 agaacttgcg ctcgcgttcg atctgttcgg gcttgcgcgt cgagcctgct cccgccgcag
    12601 actcgaacca gccgacggtc gtgtcgttgt cgtgcgtgcc ggtatagacg gcgacgttgc
    12661 gatgatagtt gtgcggcagg tctatgtttt tgctgtcgcc gccgaaaccg aactggagga
    12721 tgcgcatgcc ggggaagttg aaatcgtcgc gcagggcttc aacgtcgggc gtgatgacgc
    12781 cgaggtcttc ggcgaggatg ggtgcgtcgc cgagcgcgct tttgatcgcg gtgaagagtt
    12841 cgcgtccggg cgcgttgacc caatgcccgc gctcggcggt ctggtcgccg cccggtatct
    12901 cccaactcgc ggcgaacccg cgaaaatgat cgatgcgcag gatgtctacg gtgtgcagca
    12961 tcgaacggac gcgcgcgatc caccagcgga aatcttcggc ttgcaggcgc gcccaatcgt
    13021 agagcgggtt gccccagagt tgacccgtcg cgctgaaata gtcgggcggc acgcctgcga
    13081 cgacgcgcgg gctaccgtct tcgttcaact tgaagagatg cgggttcgtc cacacgtcag
    13141 ccgaatcgtg cgcgacgaag atcgggatgt cgccgacgat agagatgccg cgctcgttcg
    13201 cgtatcgctt gagcgcgtgc cactgtctga aaaagaggaa ttgatagaac ttgtgcgcgt
    13261 cgatctgttc gcgcaagcct tcgcgggcgg cggcgagcgc gtgcggttcg cgcttgagca
    13321 tcggcctctc ccacttgttc cacgccacgc cgccccgcgc atctttcatc gcgcgataga
    13381 gcgcgtagtc gtcgagccag agggcttcgt gctggctgaa agtctcaaaa cttccgcgca
    13441 gtccggtgtt cgtcgtgtgg cgaaaattct caaacgcgcg gcgtagcagt ctgttcttga
    13501 actcgatggc cgcgccgaaa tccacgcgtt cgtccgcgaa ctccggtgcg cccgcgaggt
    13561 cgcgttggtc taacaaattt tctgtgacga gacgttcggg gctgacgagc agcgtgttgc
    13621 ccgcgaaggc cgagaaggac tggtaaggcg aatcgccgta gcccgtcggg ccgagcggca
    13681 ggacttgcca gaggctctgc ccgctcgcgg cgagaaagtc tatgaaggca taggccgcgc
    13741 tgcccagatc gccgacgccg tatgagccgg gcaaactggt gggatgcagt aggataccgc
    13801 tcgaacgtgt aaagctcaca ggataatcct ctttgttgtt gaagtgaact cagcttaata
    13861 gtccacgcac ggcttgtcaa aaatttatcc cacgcaaacg caactcgcct tatcgatcct
    13921 ctgcgcctac gggtttttct cttctgtgcc tccggcgagc aatagagttt gcgccggggg
    13981 cgcacggtta agacggaagg cgcgcagtag ttctcagcgg cgcgcgcgga atgatttacg
    14041 ggacgctgac agcggagtag ccgggtcgcg cacggacgtt tgcgcctttg cgcgtggagg
    14101 tgatcttgat cttgcgataa ctgccattct tcacagggtt cgaagggtaa taaccgatgg
    14161 catattgcgt cctcaactcg gcggcgatct ctttgaaggc ggcgggcagg cgttcgagcg
    14221 tgtcggcgcg cgtcaacttg ccgccagact tctgcgccaa ctcgttcaaa taactgtcgg
    14281 cggtcttgta gagcgagttg agcatcatgc cgatgccgtc ttcatgcgaa gttcccggcg
    14341 cgctcacttc acccggagag cgcgggaggc gcggatcgtc gcgtcgcgtc tggcgcggat
    14401 catcgtcgcg tcgcggcggc gcggggatcg tcgggatggg gatgccgcca atcgtgggtt
    14461 gtccatcgcc tctgccgctg ggaattgtgc cgccgggaaa ggtcgtgccc gtggggatgg
    14521 gcaaagagcg gccatcgcct cgccccttga tgatggcggc gaggtctgga cgcccgccgc
    14581 ccgcagcctg ctcgcgcgca atgcgttccg tctcgattcg cgtgtcgtac ctgatcgggt
    14641 agacgatcac gcccgactct tcgagcgcgc ggatgttgtt gatgtaacga aaactgtcgc
    14701 tgcgataatc aaccccgtcc gtgaagatga cgactgcttt gcgccgcgcc ttgtcgggct
    14761 tcaaaaggga gagcgcggtc tgcatcgcgt cgtagagttt cgtgcccttg cctgcgcgcg
    14821 tcgcttgaat cgcgcgctcg acgattgcgt ggtcgctggt gaaagggttc aggtcgcgca
    14881 cttcgtcgtc gaacgacatt accttgatgc gatcctgcgg ctgcaaacca ctgacgaagg
    14941 cgttggccgc gcggcgtatg tcgcgcagtt tctcctcggt gctcgcgctg gtgtcgagca
    15001 tgagaatgac tgtgaagggt tgtgagacgg tcgagaagaa agccaactcc tgccgcacgc
    15061 cgtcctccgt cagcgtgaac tcttccttcg acatgtccgg cacgtagacg cccgcgcggt
    15121 cgctcaccac gacggggatg gagacgaggc tcgtgtcaac tttaacgact tcgccgtcct
    15181 cctgcggttc gacaacgggc gcatcctcaa tcttagccgg agcggagact gcgggttcct
    15241 tctgccggtt aggatcgacg ttcttcttct gctgactcgc ggaagagagc ggcgtgcaga
    15301 gcaatgcgag aatcaggaag agcgcaaagg ctccgggctt gagcatggac gaaaactcct
    15361 gtgcgaccaa aaggacggtt gatacgaaaa gaagaggacg cgctaaacaa tctgcaagtc
    15421 agatgtgacg cgggcgaaat atcgttgctc acaacttcgc gcggcgcaca aaacagcgtg
    15481 cgggagtttt gccctgtgcg aatgaaaaag cctctcaggc gggctcagcg tgctttcgcg
    15541 gcgacgagat aagcgaccgc cgtgcgcgcc ggaacttcaa acgtgatctc gccgccctcg
    15601 atcctgacac gctctttggc gacgagcagc ggcacgagtt ccgcgccgtc ggcgacctct
    15661 acaaacgcgg cgggcgcgct gattcgcttc gctgcggcgg cgcggttgaa ggcgaggatg
    15721 atcgtctcgt tcgccgtccg gcgtgcgaag gcgtaagaat tttcgtcaaa ggcgaggtcg
    15781 agcaggcgac cgtggcgcat cgcggcgtgc tcgcgccgga gtttcaacca gtcgcgcgtc
    15841 cactcgaaca tgcgcgcctc gcgcgcggtg cgcccgcgtc gctcgaaagc gttgcgcgcg
    15901 tcgccgctcc agccgccggg gaagtcgcgg cggttatccg ggtcgtcgcc gccttccatg
    15961 aaaatctcgt cgccgtagta aagctgcggg atgccgcgca cgctgaagag aaacgcgtga
    16021 tgcagcatag agccttcgag cgtcatgccc tcgacggaag cgatacggcg cacatcgtgg
    16081 ttgttcgtca tcacgacgag ccgcgcggca tcctcgtaga gcgcgtcgtt tttgagcatc
    16141 gagcggagcg cgttgacggg agccttgttc gtgaaggcgt cgagcgaagt ttgccagagg
    16201 ggaaagtcga agagcgaatc gagttcggta tcaatgccgt cccacccggc gcgcccgccg
    16261 aagaaatagg agagatgaac cggatcgcgg tcgaacactt cgcccaccat ccacatcttc
    16321 ggatactggc gacgcagcgc gaggctcaac tcgcgtatga aggcgcgcgg catgtactgg
    16381 atcgtgtcct gccggatgcc gtccatcccc gtgatgccga cccaccagag cgcgttctga
    16441 atttcgtagc gcgccacttc gggttcttcc tgattcatgt ccgggagatc gtcggagaac
    16501 cagccgtcga gcgtggggcg gcgcgcggct tcggaggcat gcggcgagag caggaggtcg
    16561 ccgcgaaaag ggttctgcgt gtggcgcgcg agcgtgccgt gaaaccagtt gtcgagcggc
    16621 ggatcgttga cccacgggtg ctgcgagccg acgtggttgg caacctgatc ctgaatgatc
    16681 ttgatccctg cggcgtgcgc cttttcgacc agttcgcgca gcgtcgccat gtcgccgaag
    16741 cgatcctcga cggcgtaata atctatcgcg tgatagccgt gatagtaggt gttcgggcac
    16801 cagggctgag cgcaggtttt gacgccgttc cagttgtcgt accagggatt gagccagagg
    16861 gctgtgacgc cgagttcttt caggtagggc aggcggttga tgatgccgcg caggtcgccg
    16921 ccgtgaaagg cgcgcgggtt cctgcggtcg gtcgcctcct tcggcgcgcc cggtgggatg
    16981 tcgttcgcgg cgtcgccgtt cgcaaaacga tccggcatga tgaggtagat cacgtcgtcg
    17041 gtcgtgatgc cctgaaagtg cgtggccgga tcgagcggca cattcaagcg aaaggggatc
    17101 gaagtcttgc cgccgcgcgt ctccagcgtg agggggtaat cgcccggcgc ggcgtcgggg
    17161 ctgatgcgga cgctcacgaa aacatacgtg cccgccgcat tgacgaccgc gccgcccggc
    17221 tccaactcac gtcgcgctga cacgacgcgt gcgccgtgca ggttcgtgcc acgcacgagc
    17281 aggcgcacgg ggttgatcgt gtgcgccgcc caccaggaag gcggctcgac tttggcgacg
    17341 acgggcgcag ttgagttttg cgcgtgcgcg ctcgcggcga gacaaacgag cgcaaagatg
    17401 gcgaggagac tggaacgagc gcgatgtttt gctggcattg aaagtggcat ttatctctgg
    17461 cgacgcttcc gcattgaaga tgtcttgaag atgtttttaa cccgccccgg cgagacttaa
    17521 aacggacgcc ttcgccggag acttcaccgc gagaatatca cttgccgttg aacgtcttga
    17581 cgaaaacgac gagcgcggcg atctcttctt tggagagttt tttgccgaag gcaggcatct
    17641 ggttgcgccc gtcgcggatg gctgcggtca ggcgtttgtc gcccacacgc tctttcttcc
    17701 accatttagc gtcggccaga ttcgccaccg agaagacttt gcccatcggc gtctggccgc
    17761 gcccgtccgc gccgtggcag cgcgcgcaat tctccatata taagaccctc gcgttcgcca
    17821 gttgttgctt gcgcgataac ttcttctgcg cagccgcagc cgccctcgcg ccctcttccg
    17881 cacgcgcacg acgaccggaa tagctgccgc tcaagaagag tccggcgagc gacaacgcga
    17941 cgatgatcaa cttccctctt cttctcatga tcacacttta gaacggacgc gctactccac
    18001 gcccgcgccg gacgacgcgc ccgtggcgtt tgtcgtcgct ttcccttcgc tcaagtgatg
    18061 aacgtgccac gacacgctct cgctgctcac ctcacgccgc ttgcgttcta accttaaacc
    18121 gaccgaccgc aaaactattc acataaaaaa gcaggcttcg ccgcgcgggt tcgcgtcggg
    18181 caggacgcgt ggacgacgag cgggcgacac atctagcgcg gcggtttgac cccctcgcct
    18241 gagacggagg cgggcagggg cgcgggcgcg ggcttgtcgc cttcgtccgt cgcgcccgtt
    18301 gccgcttccc cgccgagcgg cgtcgggtcg tgtttgcgcg cctcttgaaa acgtttgaag
    18361 atggcaagct cgcggtctgc gtcagacttg cgcttgaggc gcgagtaggc cgtgaagagt
    18421 tggtaatgaa tgccggggtc gttggcgttc aaggccgcgc cgcgttcgag cggcggcaag
    18481 gcttcctcgt aacgtttcag tttgacgagc agccgaccga gatcgtgatg cgcggcgaag
    18541 ttttgcgggt cgagcgcgat ggcctgccgg agcagacgct cggactcttc cgtctgcccg
    18601 cgctggttgg cgatgtagcc gagattggtg agcacgtcgg ggttatcggg ttggagcgcg
    18661 agcgattggc ggaaggccgc gagcgcgtcg tcgtactgcc cttcggcgcg ggcagcgtag
    18721 ccgatcaggt agtagagatt ggcgttcgcg gggaaacgct tgagggcatt cgcgaacact
    18781 tcgcgggcgc gcgggtaatg cttgctgcga aagttagcga cgccgagacg aatgtgatag
    18841 atgagcttcg tggaatcctg ttcgagcgcg cgctggtaaa acgggatggc ctcgtcggtc
    18901 gcacgccgtt tgagcaactc ttcgccgatc atgaagttcg cttcggggaa cgcgggacgc
    18961 agagcgaggg ctttacgcca gagttcgacg gcggcttgcc cgtcgttgcg tgcggcggcg
    19021 acgaggccga gacggtagta gggttcggcc tcgttcggcg cgagcgcggc ggcggcttcg
    19081 agcgcgcgtg tcgcttcgtc gagccggtcg agattgtaga gcgcgacgcc gaggttatag
    19141 agcacctcga aggtctgcgg gcgcaaggca ttggtgcgtt cgaagagcgg cacggcgcgc
    19201 gcatactcgt gcgcttcggc caggctgaga gcgagcgtga acaggacgcg cgggtcgtcg
    19261 tttgccagcc gctcgattcg ttcggccaac ctgcccgcgt cgtcgctgcg cccgtcgtgc
    19321 gcgtaggcgt tggtgagcgt gagcaggagc gcaaggtcgt ttgaagaggc ggcgggactc
    19381 gcgggattga ttccggcggc gcgttcgagc agggggatgg cgcgtttgta atcgccgcgc
    19441 gcggcgtaga gcgcgccgag attgaagacg gcgaaagcgt tttcgggcgc gacgcgcaac
    19501 gcagcctcga aactttcgat ggcctgttcg acgcggttcg tgcgcgagag caacacgccg
    19561 agattggcgt gcgcgggagc gtgctgcggg gctgacttga tcgcctcgcg atactcgcgt
    19621 tcggcttcga ccgcgcgccc gctcttgtct agaacgacgc cgaggagcgt gcgtgcgttc
    19681 ggatcgcgcg gcgcaacatt caagaccttg cgcgccgccg cctccgcttc gaccagtttg
    19741 cccgactgca acagcgcggc ggcttcgcgc aggagtgacg ccgttgcggc gtcggcttgg
    19801 ccgcgcgcgg cgacgggaga ggttggtcgt ccggctgtgc ctttggctgt gccttgctgc
    19861 gttgcggcgg ctggtcgcgc agcggtcggg gcgcggcgtc tctgcgctgg cgcgggcgcg
    19921 gcgtagattc ctgtgagcgt caggcaagcg aggaggcgcg ccgcactctg tagcaggctg
    19981 tcaggcgaac ggcgcgtggg cgattggtat agcattagat caatgaccgg ataatctcag
    20041 ttggcctgcg gtcgtaactt cgcagacaac tcgccgggga aagtttcagg ttaaaggctt
    20101 ccgcacgtga tctcaagcga cgccgggcgg agaagcgcgg gcgataaagc aatcgagtgg
    20161 cgagcggata atgcttccgg ctcgccactc gatctccaac gttgcttg
//
LOCUS       DASWNS010000265        10878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_347610, whole genome shotgun sequence.
ACCESSION   DASWNS010000265 DASWNS010000000
VERSION     DASWNS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347610"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..844
                     /locus_tag="VGB76_09400"
     CDS             224..844
                     /locus_tag="VGB76_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0099156.1"
                     /translation="MRRTLLSLTFLFLLSSLAYADPLIITGGTANILGPVSGPAVGSF
                     NLLAPGFSATGSGDRNSANGLTSINGSSSFRGSFTSGETSGSGVVYLNTMLNFTLAPF
                     TPPDPGSNSDSVFVSTAFTMTGVVDIRAFAVGLPVLFNSDVAGSGFALITYARLAPNL
                     FVPMNVTYYFGAAEPIPEPATLVLLGTGLAGIAAKVRRRRRAAGEA"
     gene            1021..1932
                     /locus_tag="VGB76_09405"
     CDS             1021..1932
                     /locus_tag="VGB76_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099157.1"
                     /translation="MSGNILARRTAHRWSVSSGLLFRAGLATLLFFMLTVGANAYTLV
                     LRSGRVVTVSDNFKVTPTAFTYEASPGYWVTVWLSNVDIAATERANNEPAGSFKRRIE
                     QEPEKTPAAAARVPQGVEAGRAAGRKVITNKELEPTRLMRRAQEEEYERTRRERGMPS
                     RQEMQQRIEEHDRWLSEWAQRMQEERREAELESLRSELANVRLQLSELDFGLSQQGTT
                     YVPAYVTPNYYPYFYAPPAQIINALPFGHRGLYGRAKFGRHAHKRSWLYQPRPGRHFP
                     RVNRPFHGPGGLRRAMPPGMAAPRHAR"
     gene            complement(2012..3640)
                     /locus_tag="VGB76_09410"
     CDS             complement(2012..3640)
                     /locus_tag="VGB76_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY0099158.1"
                     /translation="MAHTFSRRDFLRTSLVGLAGATMLGPGIISGLAANSEPVEAIVI
                     GSGFGGAVAALRLAQAGVRTLMLERGRRWTVTPEQNTFATLPNPDGRAAWLSSTTVFG
                     NPMPIDVYTGVLERTIGDGVTVYAGAGVGGGSLVYNGVTYQPTREMFQRSFGRTISYD
                     EMEETYYPRVRSMLNPSQLPADILATDYYRMTRIFLEMADKAGLHGRLADMNIDWNVV
                     RGEIAGTHVPSALIGEFWYGNNSGCKNSLDRNYLAQAEASGYLQIMPLTVVTEITEAP
                     GHRYVVTCDQIDETGTPQRRMQLTCNQLFLAAGSMGTSKMLVKAKAKGTLSKLNEHVG
                     RGWGNNGDFFGMQVGLPDTNPSQGGPASAIIEDHNNPLGPTAVMCAPQWDAQGGVINT
                     LGLSIPPALGVFGYDAATDSVKLSWPGNDPRIARTVEAGQQTYETLNRANTTDVYQPS
                     TAFVAGGLTAHPLGGAVLGRACDKYGRVEGYQNLYVVDGALIPGSSGCTNPSFTIAAL
                     AERCMEKIVPEIVEKNGRRLFAVASARGRMGARA"
     gene            4147..4374
                     /locus_tag="VGB76_09415"
     CDS             4147..4374
                     /locus_tag="VGB76_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099159.1"
                     /translation="MQIVDQLTHGTYLDLRGALPGISLVQVIHVQDEASLEEALRVAP
                     ETDALLLDSGNQRVAVKLLGGTGGAHTRLDD"
     gene            4334..4564
                     /locus_tag="VGB76_09420"
     CDS             4334..4564
                     /locus_tag="VGB76_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099160.1"
                     /translation="MEAQGGRTHDWTISRRIVERVKKPVFLAGGLKPENVREAIEQVA
                     PFGLDVCSGVRTEGRLDERKLSAFFEQIDSCR"
     gene            complement(4743..6851)
                     /locus_tag="VGB76_09425"
     CDS             complement(4743..6851)
                     /locus_tag="VGB76_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0099161.1"
                     /translation="MKRLTTNWYAEDSFRQRVKSLFVEHQQSICKRTDRMFAVLMLIQ
                     WMAAIAAAFWLSPKTWAGAESQTHIHVWTAVFLGGVISLFPISLALTRPGEASTRYVI
                     AVGQALMSSLLIHLTGGRIETHFHVFGSLAFLSFYRDWRVLVPATVVITADHFLRGVF
                     WPESVYGVLTVSNWRWLEHAGWVLFEDTFLLIAIKRSMSEMCDIAERTAESERLNEGL
                     EQRVSERTEQLAATNRELEREVDERRQVEEQLLHNAFHDVLTGLANRALFMDHLKLAL
                     GRAERQEAYQFAVLFLDLDRFKVVNDSLGHMVGDGLLIEIARRLEAAVRPGDTVSRLG
                     GDEFTVLLDGLCDSAEARAVAERLQSELAVPFNLGEHEVFITVSIGVAIGATGYQRPE
                     EILRDADTAMYRAKQLGKARYEMFDEAMHTRAMDRLGLERDLRRAVERQELFLQYQPI
                     VSLGSGSLRGFEALVRWQHPERGIISPVEFIPLAEETGLIIQIGGWVLREACRQMSRW
                     QDIPRQDKPLQISVNLSCKQFLQPDLLEQIQAVLQETGLDPRSLKLEITESVVMENIE
                     TATSTLERLQAFGVELSIDDFGTGYSSLSYLQHFPVSTLKIDRSFVSRMTESAGTAEI
                     VRTIMHLGQNLGMNVIAEGVETEQQSAQLKALGCEFGQGYYFSRPMDSDAAEALFLGR
                     FPENGLQLPLINGSTSPVSA"
     gene            complement(6832..7473)
                     /locus_tag="VGB76_09430"
     CDS             complement(6832..7473)
                     /locus_tag="VGB76_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099162.1"
                     /translation="MNFKKNRLTLLALSALWLTMICTGMWFLKNYEISPGVAAAAPDR
                     WPDGSRIRRAADRATLVMLAHPHCPCTRASIGELSRLMTQARGRVTAYVLFVKPLNFS
                     DGWEQTDLLRSAAAIPGVNVVRDDQGVEAARFRAATSGQTMLYDAEGKRLFSGGITSA
                     RGHAGDNAGRTAIVSLLTNGETEHSDTPVFGCPLFAETECPAGKEVGHEKANH"
     gene            7873..9282
                     /locus_tag="VGB76_09435"
     CDS             7873..9282
                     /locus_tag="VGB76_09435"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0099163.1"
                     /translation="MKTLLMVSLLLCLPVVGLAQAKKGAPKKKASPASTQQRKIFPYA
                     YTIDDLPNGLRLVTVPTDYPNLVALYIVVQTGSRNEIEPGKSGYAHFFEHMMFRGSKN
                     VTPAQRDDILKRAGASSNAYTTDDRTVYHEVFSKEDLDEVMRIEGDRFQSLQYAEPEY
                     KTEALAVLGEYNKNSASPGSKLFEKLRETAFRTHTYSHTTMGFIKDIEDMPNQYQYSL
                     DFYRRYYRPEYTTILVVGDVTRQRALALTRQHFGAWERGNYKPDIPAEPAQTEPREAH
                     VEWPSATLPYLAVAFRAPAYSDESKDKVALDLLSAIAFGSNSELYQRLVLKEQKVETI
                     GVSFGDKADPELFTVQTRIKQVQDMPYVREQILATFKRYTTELVPQQKLDDTRSRRRY
                     GFAQAMDSSEAIAGALAPYLALRRTPETMNKLFALADSVTPADIRDAARRYFTDNNRT
                     IVTLSQGAGKQAANKEGDK"
     gene            9285..10853
                     /locus_tag="VGB76_09440"
     CDS             9285..10853
                     /locus_tag="VGB76_09440"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0099164.1"
                     /translation="MRQITMRRIFSITLALSFMLSTMTANAQRKSPRPKNSASGNAAT
                     MKTAAASNTVLLPNRSPLVTFRILFMTGAADDPAGKEGVAALTASLLSGGGTRALAYE
                     KIQEAFYPMATSFNSQVDKEMTVFTGTTHADNLTRYYELISQMLLDPGFREDDFARIK
                     TNTINYLKVSLREGNDEELGKEQLYNNIYAGHPYGHHNYGGIGALGRITLEDVREFYR
                     THYTQANLVLGLAGGYPQTFPAKVKADFAKLPAGTPTPSNFFTPKLEPGMHIDIVKRE
                     TRSTAISLGFPIDVTRADRDWPALAVVASYFGQHRSSNSYLYQRLREARGLNYGDYAY
                     IEYFPGGMFQFEPDPNLARQQQIFQIWIRPVPPEQGHFVLRAALYEYDKLVRDGLSRE
                     AFEATREFLTKYSNILTATQDAQLGYALDSRYYKIGDFNRYMRDQLSRLTLEEVNAAI
                     KKYLATNRMRIVVVTKDAEGLRDAILANKPSPMTYNSPKPQEILDEDKIIQAYPIDVR
                     AEQVRITPVERVFP"
BASE COUNT         2209 a         3366 c         3164 g         2139 t
ORIGIN      
        1 cggtgatcat atccgtaata ggaagtcgtt aagagtggca gggttggaaa agagtggcag
       61 ggtgggaaaa ttattggagc gcgtagagat gcgtgcgccg tcgagttttt tccgttcgcc
      121 tttcactcaa agagctttcg ctggttgtcc ctttcaggat atattcccgg cacaaagcca
      181 ttccagaggg aatcaccctc tgagctgtaa aggagatacc ccaatgcgcc gcactctctt
      241 gtctctcacc tttctcttcc ttctttcgtc tctcgcatac gccgacccgc tcatcattac
      301 aggggggacg gcaaacattc tcggccccgt gagcggcccc gctgtgggca gcttcaacct
      361 gctcgcgccg ggattttcgg caaccgggtc tggagataga aactccgcta acgggttaac
      421 gagcattaac ggcagctcaa gttttcgcgg ttcctttacg agcggcgaga cttcggggag
      481 cggcgtcgta tacctgaaca cgatgctcaa tttcaccctt gctcccttta cgcctcccga
      541 tcccggtagc aattcggaca gcgtctttgt gagcaccgcc ttcaccatga cgggcgtggt
      601 ggatatacgc gctttcgccg tcggcctccc ggtactcttc aactccgacg ttgccggaag
      661 cggcttcgcg ttgatcactt acgcacgtct cgcaccgaac ctgttcgtcc caatgaatgt
      721 cacctactat ttcggtgccg ccgaacccat acccgagcct gcgactttag tgctgctcgg
      781 caccggactg gcgggcatcg cggcgaaggt aagaaggcgg cgcagagcgg ctggcgaggc
      841 gtagcccgcg tgcgccgtag ggctatctaa aatgtcggca tgtttcgaca accggaagag
      901 gagcggggaa tcgcgttgga tagggcagga gtttcggcgt tgagtgctgt ccgggagcgt
      961 cgcggctgcc gtatgttatc atccgcacaa acattcattt ccggaagggg gtatcccgta
     1021 atgtcaggta acatcctcgc gcgtcgcacc gcccacagat ggagcgtctc gtccggccta
     1081 ctgttccggg ctggcctagc aactctgctc ttcttcatgc tcacggtcgg ggcgaacgcc
     1141 tacacgctcg tcctgcgcag cggtcgggtc gtcaccgtct ccgataactt taaggtgacg
     1201 ccgacggcgt tcacctacga ggcgtcgccg ggctactggg tcacggtgtg gctgtcaaac
     1261 gtcgatatag cggcgaccga gagggctaac aatgagcctg cggggagctt caaacggagg
     1321 atcgagcagg agccggagaa gacgcccgcc gccgcagcgc gagtgccgca aggcgtggag
     1381 gcggggcgtg ctgccggtcg gaaagtcatc accaataaag agcttgagcc tacacggttg
     1441 atgcgccggg cgcaggaaga ggagtacgag cgcacacgcc gcgaacgcgg catgccttca
     1501 aggcaggaga tgcagcagcg cattgaggag cacgaccgct ggctgagcga gtgggcgcag
     1561 cggatgcagg aagagagaag ggaggcagag ttggagtcgc tcaggtctga gctggcgaac
     1621 gtcaggctgc aactgagcga actggatttt ggcctgtcgc agcaggggac gacctacgtg
     1681 cctgcctacg tgaccccgaa ctactatccg tacttctacg ccccgcccgc acagatcatc
     1741 aacgccctcc ccttcgggca caggggactg tacgggcgcg caaagttcgg acgccacgcg
     1801 cacaaacgct cttggcttta ccagccgcga ccgggtcgtc acttcccacg ggtcaacaga
     1861 cccttccacg gaccgggcgg tctgcggcgg gcaatgcctc cggggatggc tgctccccgt
     1921 catgcgcgtt gaggcgaact gcgcgggact acaaacaagg atagataagc gggggcgagc
     1981 cgtgattggc tcgcccctgt gctttatgac actatgctcg cgctcccatc cttccgcgcg
     2041 cggacgcgac ggcgaagagt ctccggccat tcttttcgac gatctccggg acgatctttt
     2101 ccatacaacg ttcggccaga gctgcaatcg tgaaagaggg attcgtgcag ccggacgagc
     2161 cgggaatcaa tgccccatcg acgacgtaaa ggttctggta gccttcgacg cgcccgtact
     2221 tgtcacaggc tctaccgagg acggctccgc ccagcggatg cgccgtcagt ccgcccgcga
     2281 cgaaggccgt agagggctgg taaacgtctg tcgtgttagc cctgttgagc gtctcgtagg
     2341 tctgttgtcc ggcttcgacg gtgcgcgcga tgcgcgggtc gttgcccggc catgagagtt
     2401 tgacggaatc ggtcgccgcg tcgtaaccga agacgccgag agccggggga atcgacagcc
     2461 cgagcgtgtt gatgacgcct ccctgcgcgt cccattgcgg ggcgcacatc acggcggtcg
     2521 gacccagagg gttgttgtgg tcttcgatga tagccgaagc cgggcctccc tggctcggat
     2581 ttgtgtccgg tagtccgacc tgcatcccga agaagtcgcc gttgttgccc caacccctgc
     2641 cgacgtgctc attcagcttc gagagcgtgc ccttagcttt ggccttgacg agcatcttgg
     2701 aggtgcccat cgaacctgcc gccagaaaca gttgattgca ggttaactgc atacggcgtt
     2761 gcggcgtgcc cgtctcgtca atctggtcgc aggttacgac gtacctgtgg ccgggggctt
     2821 ccgtaatctc cgtcacgacg gtgagcggca tgatttgcag gtagccgctc gcctcggcct
     2881 gcgcgagata gttgcggtcg agactgtttt tgcagccgct gttgttgccg taccagaact
     2941 cgccgatgag cgcggaaggc acgtgcgttc ccgcgatctc gccgcgcacg acgttccagt
     3001 cgatgttcat gtcggccaga cgcccgtgca gccctgcctt gtcggccatc tcaaggaaga
     3061 tgcgcgtcat tctgtaatag tcggtcgcca gaatgtctgc gggcagttgt gaagggttga
     3121 gcatggagcg cacgcggggg taataagtct cctccatctc gtcataactg attgttctgc
     3181 cgaaggagcg ttggaacatt tcgcgcgtgg gctggtaggt cacgccgttg tagacgagcg
     3241 agccgccgcc cacgcccgcc cctgcataga cggtcacccc gtctccgatg gtgcgttcca
     3301 gcactcccgt gtacacgtca atcggcatgg ggttgccgaa cacggtggtc gaactgagcc
     3361 aggccgcgcg cccgtcgggg ttgggtaagg tggcgaaggt gttctgttcc ggcgtcactg
     3421 tccagcgccg cccgcgctcc agcatcaacg tgcggacacc cgcctgcgcc agacgcaggg
     3481 cagccaccgc gccgccgaat ccactgccga tgacgatggc ctcgaccggc tcgctgttgg
     3541 cggccagacc actgataatt ccggggccga gcatcgttgc tccggccagt ccgacgagac
     3601 ttgtgcgaag aaaatcccgc cgtgagaagg tgtgagccat tgaaattacc tctataaatt
     3661 gtcttttttt tgagagaact ggagcggcgc ggggcgagtt tgaaatagta aggatcaata
     3721 cccgaatgcg ccctcaaccc ttaacgcgaa ttttttgggg gaaccggatc acacggctga
     3781 attttttttt gcacgccgtc gctcgccagc cgttttggga agtttcgagc agtctgcgca
     3841 agtgagttta aggttgattc cgcacgcagg ctcgcgccgc ttgaacggga cggcgaaacg
     3901 gactcggcct gttcagggag gtctgaaaac gtgtgagcgt gctacgggtt tataatttgt
     3961 gtcgtcgctt cgccggaatc cgctgtgggc gttggactcc gacgccagat cgggttgccg
     4021 gtgtttctga gtagactctg aaaacgcggt tcatcgcgca ggatgtcgaa ggcaggcttg
     4081 agaaatttat agaggaggct ctatgaaatt tgcgcagcgc ataaacgcta cgtcggaagc
     4141 aacgcgttgc agatcgttga ccagctgacg cacggaactt accttgatct acgcggcgcg
     4201 ctgccgggca tcagcctagt gcaggtcata cacgtgcagg atgaagcctc gcttgaagag
     4261 gcgttgcgcg tcgcacctga gacggacgcc cttctgctgg attcaggcaa ccagcgagtc
     4321 gccgtcaagt tgcttggagg cacagggggg gcgcacacac gactggacga ttagccgccg
     4381 catcgtcgag cgagtgaaga agcccgtctt cctcgccgga ggtttgaagc cggagaatgt
     4441 gcgtgaggct atcgaacagg tcgcgccgtt cgggctggat gtttgtagcg gggtgcgaac
     4501 cgaaggcaga ttggacgagc gcaagctgtc ggctttcttc gaacaaattg atagctgtag
     4561 atagttcttt gtcccttatg ctgctcgccg ggaacggaac tcccgcacgg ttcgacgcag
     4621 caccaggaaa ccctactcta cgcggagact aaccatcgtt atgaaagttt acaaaccgga
     4681 gtttcgtgag gccgggtctg gaaagcgggc tgactttctg tgctgaattt atggggtgac
     4741 aatcaggcac ttaccggcga cgtagagcca tttatcaagg gaagctgcaa gccgttttcc
     4801 ggaaagcgtc cgaggaaaag cgcttctgcg gcgtcgctgt ccattggccg ggagaagtaa
     4861 taaccctgcc cgaactcaca cccgagagct ttgagttgcg cgctctgctg ctccgtctcc
     4921 acgccctcgg ctatcacatt catgcccaaa ttctgtccga gatgcatgat cgtccgcacg
     4981 atctccgccg tccccgcgct ctccgtcatc cggctgacga atgagcggtc gatcttcaat
     5041 gtgctgacgg ggaaatgctg caagtagctg aggctggaat agcccgtgcc gaaatcgtcg
     5101 atgctcaatt ccacgccgaa cgcctgtagc cgttcgagtg tactggtggc cgtctcgata
     5161 ttttccatca ccacgctctc cgtgatctcc agtttcaaac tgcgcggatc gagtcccgtc
     5221 tcctgtagaa ccgcctgaat ctgttcgagc agatcgggct gcaagaactg tttgcaggag
     5281 aggttgacgc taatctgtag aggcttatcc tgccggggga tgtcttgcca ccggctcatc
     5341 tgcctgcaag cctctctaag cacccacccg ccgatctgaa taattagccc cgtctcctcc
     5401 gccagcggga tgaactccac cggcgagatg attccgcgct ccgggtgctg ccagcgcacg
     5461 agcgcttcga agcctcgcag ggagccgctg cctaaagaga cgatgggctg gtattgaaga
     5521 aacagctcct gccgctcgac ggcgcgcctc aagtctctct ccagtcccaa cctgtccatc
     5581 gcgcgcgtgt gcatcgcctc atcaaacatc tcatagcgtg ccttgccgag ttgcttggcg
     5641 cggtacatcg ccgtgtccgc atcgcgcagt atctcttccg ggcgttgata gcctgtggca
     5701 cctatggcga cgccgatgct cacggtgatg aacacctcat gctcgcccag attgaacggc
     5761 acggccaact cgctctggag tcgctcggcc accgctctgg cctcagcgga atcacacagg
     5821 ccatcgagca ggactgtgaa ctcatcgccg ccgaggcgcg atacagtgtc gccggggcgg
     5881 actgcggctt ccaaccggcg tgcgatctcg atgagcaacc cgtctccgac catgtggccg
     5941 agactatcgt tgacaacttt gaaacggtcg aggtctagga agaggactgc gaactggtaa
     6001 gcctcctgcc gttcggcgcg tccgagcgcg agcttcaagt gatccataaa cagggcgcga
     6061 ttcgccagac ccgtcagcac gtcgtggaag gcgttatgga gtagctgctc ttcgacctgc
     6121 ctgcgttcgt ccacctctct ctcaagctct cgattcgtgg cggcgagttg ttcggtgcgc
     6181 tcggaaacac gttgttccaa cccctcgttg agcctttcgc tctcggctgt tcgttcggcg
     6241 atgtcgcaca tctcgctcat gcttcgcttg atggcgatga ggagaaaggt gtcttcaaag
     6301 agaacccagc ccgcgtgttc cagccagcgc cagttgctta ccgtgagcac gccgtagacc
     6361 gactcaggcc agaacacacc gcgtagaaaa tggtctgccg tgatgacgac ggtagccggg
     6421 acgagcaccc gccaatccct gtagaaggag agaaaagcca gcgagccgaa gacgtgaaaa
     6481 tgcgtctcga ttcgcccgcc cgtaagatgt atgaggaggg acgacattaa cgcttgcccc
     6541 accgcaatga cgtagcgggt cgaggcttcg cccggtcgcg tcaaagccag agagatgggg
     6601 aagaggctga tgacgcctcc cagaaacacc gccgtccaga cgtgtatgtg agtctgactc
     6661 tcagctcccg cccatgtctt cggggagagc cagaaggcgg cggcgatggc ggccatccac
     6721 tggatcaaca tcaagacggc gaacatgcgg tcggtgcgtt tgcagatgga ctgctggtgc
     6781 tcgacgaaga gactcttgac gcgctgccga aaagagtctt cagcgtacca gttagtggtt
     6841 agccttttca tggccgacct cctttcccgc cgggcattca gtctcggcaa agagcgggca
     6901 gccaaagacg ggagtatcgc tgtgctccgt ttcgccgttc gtgagaagcg agacgatggc
     6961 cgtgcgtccc gcgttatcgc ctgcgtggcc gcgcgcgctc gtaattccgc cgctgaagag
     7021 ccgtttgccc tctgcgtcgt agagcatggt ctggccggaa gtggcggcgc ggaaacgtgc
     7081 ggcctcgacg ccctgatcgt cgcgcacgac gttgacgccg gggatcgcgg ctgcgctcct
     7141 cagcagatcg gtctgctccc acccgtcgga gaaattcagc ggcttgacga aaagcacgta
     7201 agccgtcaca cgcccgcgcg cctgcgtcat aaggcgcgat agttcgccga tactggcgcg
     7261 cgtacagggg caatgcggat gcgccagcat gacgagcgtt gccctgtcgg cggcgcgtct
     7321 gatacggctg ccgtcaggcc agcggtcagg agcggcggcg gccacaccgg gactgatctc
     7381 gtagttcttg aggaaccaca ttcccgtaca gatcatcgtg agccataacg cgctcagggc
     7441 aagcagcgtc agcctgttct ttttgaaatt catcaagagc gatccctaaa atgagtcctg
     7501 aatgttttgc gagagaacct gccgggagtt aagagggcgg ggcagaagcc aaaggctgcg
     7561 aaatggcgtt gcgtgtagca gtttgaccgg aggcactgct tactacagtg caatgtgact
     7621 ggatatattg accgaagagc gaatattccc gctgtcgagc cttccccggg caattgttgg
     7681 caaagcccgt accactcaca gcttgctcga tgggtaaacg cgaggcggag ccgctcccgc
     7741 ctcccacgtc ctcacttaat tcctacgtcc tcacttaaaa ctgcggccac atcgctcccg
     7801 ccgaccgcct cgtgtataat ttccgcgtct ccatctacat catcaaaaca agttttacgg
     7861 agtttacgcg atatgaaaac cctgctcatg gtttcgcttc tgttgtgcct gcccgtcgtc
     7921 ggactggcgc aagcgaagaa gggcgcgccg aagaagaagg cgtcgcccgc gtctacacaa
     7981 caacgaaaga tatttcccta cgcctacacg attgacgatc tgccgaacgg cctgcgcctc
     8041 gtcaccgtgc cgaccgacta cccgaatctc gtcgccctct acatcgtcgt ccagaccggc
     8101 tcgcgcaacg agatcgaacc cggcaagagc ggttacgcgc acttcttcga gcacatgatg
     8161 tttcgcggaa gcaagaacgt tacgcccgca cagcgcgacg acattttgaa gcgtgccggg
     8221 gcgtcctcta acgcttacac gaccgacgac cgcaccgtct atcacgaagt cttctcgaaa
     8281 gaggacttgg acgaggtgat gcgcatcgaa ggcgaccgct tccagtcgct ccagtatgcg
     8341 gaaccggagt ataagacgga ggctctggcg gtgctcggcg agtacaacaa gaactccgcc
     8401 agccccggca gcaagctctt cgagaaactg cgcgagacgg ccttccgcac gcacacctat
     8461 tcgcacacca cgatgggctt catcaaagac atcgaagaca tgccgaacca gtaccagtac
     8521 agcctcgact tctatcgtcg ctactatcgc cccgaatata cgaccatcct cgtcgtcggc
     8581 gacgtaacgc gccaacgcgc gctcgcgctc acgcgccagc acttcggcgc gtgggagcgc
     8641 ggcaactaca agcccgacat ccctgcggaa cccgcgcaga ccgagccgcg cgaagcgcac
     8701 gtcgaatggc cgagtgcaac cctgccttat ctggccgtcg ccttccgcgc ccccgcttat
     8761 tccgacgaga gtaaagataa ggtcgcgctc gatctgctgt cggccatcgc cttcggttca
     8821 aactctgagt tgtatcaacg tctcgtcctg aaggagcaga aggtcgagac aatcggcgtc
     8881 agtttcggcg acaaagccga cccggaactc ttcaccgtcc agacgcgcat caagcaggtg
     8941 caggacatgc cttacgtccg cgagcagatt ctcgccacct tcaaacgcta cacgacggaa
     9001 ctcgtcccgc agcaaaaact cgacgacacg cgttcacgcc gtcgctacgg tttcgcgcag
     9061 gcgatggaca gttccgaagc gattgccggt gcgctcgccc cctatctcgc cctgcgccgc
     9121 acgcccgaga cgatgaacaa actcttcgcg ctcgcagaca gcgtgacgcc cgcagacatc
     9181 cgcgacgccg cgcgccgtta cttcacggat aacaatcgca ccatcgtcac gctctcgcag
     9241 ggcgcgggca aacaggccgc aaacaaggag ggcgacaaat aaccatgcga cagataacca
     9301 tgcgacgaat attctccatc acactcgcgc tctcgttcat gctctcgact atgaccgcaa
     9361 acgctcaacg caaatcgccg cgacctaaaa actccgcgag cggcaacgcg gccaccatga
     9421 agacggctgc cgcttccaat accgtgctgc tcccgaatcg ctctccgctc gtcaccttcc
     9481 gcatactctt tatgacgggc gcggcggacg acccggcggg caaagaaggc gtggccgccc
     9541 tgactgcctc gctgctctca ggcggcggca cacgcgcgct cgcctacgag aagattcagg
     9601 aagccttcta cccgatggct acctccttca actcgcaggt tgataaagag atgaccgtct
     9661 tcacgggcac gacgcacgcc gacaacctga cgcgttacta cgaactcatc agccagatgc
     9721 tcctcgaccc cggtttccgc gaagacgatt tcgcacgcat caaaaccaac acgatcaact
     9781 atctcaaagt ctccctgcgc gaagggaacg acgaagagtt gggcaaggag caactctata
     9841 acaacatcta cgccgggcac ccttacggcc atcacaacta cggcggcatc ggtgcgctcg
     9901 gacgcatcac gctcgaagac gtgcgcgagt tctatcgcac gcactacacg caggccaacc
     9961 ttgtgctcgg cctcgcgggc ggctacccgc agacgtttcc cgcgaaggtc aaagccgact
    10021 tcgccaaact tccggcgggc acgccgacgc cctcaaactt ctttacgccc aaactcgaac
    10081 cgggcatgca catcgacatc gtcaaacgcg agacgcgctc cacagccatc tcgctcggct
    10141 tcccgattga cgtgacgcgc gcagaccgtg attggcctgc gctcgccgtc gtcgcctcct
    10201 atttcggcca gcatcgttcg tccaacagtt atctctatca acgtctgcgc gaagcgcgcg
    10261 gcttgaacta cggcgactat gcctacatcg aatactttcc cggcggcatg tttcagttcg
    10321 agcctgatcc gaacctcgcg cgccagcagc agattttcca aatctggatt cgccccgtgc
    10381 cgcccgaaca aggccacttc gtcctgcgcg ccgcgctcta cgagtatgac aaactcgtcc
    10441 gcgacggcct ctcgcgcgaa gccttcgagg ccacgcgcga gttcctcaca aagtattcca
    10501 acatcctgac ggccacgcag gatgcccaac tcggctacgc gctcgatagc cgctattaca
    10561 agatcggcga cttcaaccgc tacatgcgcg atcaactttc gcgcctcacg ctcgaagagg
    10621 tgaacgccgc tatcaagaag tatctggcga cgaaccgcat gcgcatcgtc gtcgtcacga
    10681 aagacgccga aggtttgcgc gatgccatcc tcgcgaacaa gccctcgccc atgacctaca
    10741 actcgcccaa gccgcaggaa attctggatg aggacaagat cattcaggct taccccatcg
    10801 acgtgcgcgc cgaacaagtt cgcatcacgc ccgtcgagcg ggtctttccc taagcggcag
    10861 gcagggtagt gatgttat
//
LOCUS       DASWNS010000266         6262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_88729, whole genome shotgun sequence.
ACCESSION   DASWNS010000266 DASWNS010000000
VERSION     DASWNS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88729"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..2863)
                     /locus_tag="VGB76_09445"
     CDS             complement(74..2863)
                     /locus_tag="VGB76_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY0099165.1"
                     /translation="MAVRHPPGNHVPPPAAKRRGARVIRYEEPDEGFFRRWRRRIFRP
                     PLVIALVILTTFTIGVLGYYYYIFSERVDRLISGEIFTRSAGLYTAPKELHVGAGLSA
                     DELVKRLQRAGYVERAAQADEARGRYVSNGAAVEIEPGQNAIVDGQRLFPRVRVSFAR
                     NGKGITAINDLDSKTKLEHALIEPEQISFVTGSDRQKRRIIGFQDIPPHLVKAITVTE
                     DRTFFEHYGINIRGILRALVRRYDANPNSPIARQGGSSITQQLVKNLLLSPEYSLRRK
                     LAEAYMSIILETRLNKQEIFALYCNEAYLGQHGGFSINGFGQATQVYFNKDVTGVTLP
                     EAAFLAGIIRSPNRYNPYRNLQTATARRNQVLDSMAEAGAITEEEAARAKATELKVAP
                     ARGRLDSSEAPYFVDYVQAQLADILADARTAEHLRIYTTIDMELQRAAYAAVTKQLAA
                     LDKIYAKRGKNSVAPGTLQASLVAMNAQTGEVVAMVGGRDYERSQLNRATDAMRQPGS
                     VFKPFVYATALNTAYDPVPRVITAATTYMDEPKTFTYDNQEYSPGNFGDSYANAPVTL
                     RDALVHSKNVITVDVAMEVTIGRVMNLATRAGLPKPQRAYPAMALGTNEATPLQVASA
                     YTTFARLGTRTTPIGINRVTTGRGATIAAPATQQNDVMRPEVAYLMTSMMKDVVNRGT
                     AARLRGRGFKFNVAGKTGTSRDGWFAGYTPQLVCVVYVGFDDGSPLGLTGADSALPIW
                     ADFMQSALSAHPEWAGDWQMPPGLEEASIDPRTGLLAVADITAGKRTELFITGTAPTI
                     ESNAPPEDELAPLDGTGEEGGEFDYAPAPVIVPSDGSDPPPPADAPPQRPRTTTPRLE
                     GRGGAQTDGSSRLMGTITLDIDPTTGLMAASTCPVIRSKTFSIGQEPRRYCGPEYHNG
                     KTILPSGTRPRLVLP"
     gene            3044..4081
                     /locus_tag="VGB76_09450"
     CDS             3044..4081
                     /locus_tag="VGB76_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY0099166.1"
                     /translation="MSSTHHLPHLSKSARRRTLYALAALVALIAIGTIGFQLLEPLSL
                     LDSLYLTVMTVATVGYGDVHPVSRGGRAFSIGFMLVSVAAVGFLLSTAIQALVQSEIV
                     ATYDQRRRSREINKLRNHFIICGAGRVGSRIIRELQRREEPFIVIEREERNVASLIEE
                     DVLVLVRDATLEETLRDAGVEHARGLAACLPDDADNVYVVLTARGLNNDLHIVARAVE
                     EQAEPKLIRAGANRVIAPVIIGGQRMLQALTKPAVSDFIDSIAAEDLDLDFEEVVVSP
                     ASAYVGHKLKFTNIRSELDVVIVALRRSSNSEMIFNPSGDALIESGDMLIAIGRADSL
                     AKLKTQAQRKS"
     gene            4137..4979
                     /locus_tag="VGB76_09455"
     CDS             4137..4979
                     /locus_tag="VGB76_09455"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0099167.1"
                     /translation="MKVLVTGGSGYLGIHVRRFFEADDFSRRVGLDILDPYDASRAAD
                     YDAVIHLAAHLDKNPEGAADCFRTNAEGTANLLTHMREGATFIYASTKDVYGAHADGY
                     KEVPESCATEYCGQSPLEWSKLIGERYVEYYAAARGIRACIFRLSAVYARPSEGNESN
                     FVTHYVEAVKRGWPLSLPGGGQPVRDILHVEDFSRACRAFMDSGLTRGLYNLGGGRAQ
                     ATTLSGIVETIGRLIQLQPVIAEDARLPAPVPFNYVSDLSRARAELGWQPLINIEEGL
                     QSLL"
     gene            5129..6256
                     /gene="waaF"
                     /locus_tag="VGB76_09460"
     CDS             5129..6256
                     /gene="waaF"
                     /locus_tag="VGB76_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEY0099168.1"
                     /translation="MTLKLHTEQIERVVVRGANWVGDAVMTVAALRELRRVLPAAHIT
                     LATRPWAQDIFAGAGIVDELLLIDDTRRGVRATLRQIAQWRARRFDLALLFPNAFEPA
                     FIAAAARVPLRVGYATDGRAFLLTHPLAVPAWRSEQHEVFYYLNLVFGLEHTLTGEAQ
                     FENREPHFQLQTSSERQAAARQLLERHGALPDRPLVALCPGSTNSRAKRWPAERYAAL
                     ADHLMGKAKANVVLIGAREELDVTQEVVARMRRRPVVLTGETDLAETIAVLSVADLLV
                     TNDTGPAHISAALERPTLVIFGATNPLTTRPFSPTADIIRHPPDCAPCMLRDCPIDHR
                     CMTAITPEEVFLRAVEMLAQGQCRTHAAGDASTQEVGAEVA"
BASE COUNT         1098 a         2005 c         1953 g         1206 t
ORIGIN      
        1 ggcagtttgt acgcctcatt cgttgtctca caagctatct cactgcccgt tagaattttc
       61 gcaaacgctc gtctcaaggc aagacgaggc gcgggcgcgt gccggagggc aagatcgttt
      121 tgccgttatg atattcggga ccacagtagc gacgcggctc ctgcccgatg gagaaagttt
      181 tcgagcggat gacgggacac gtcgaagcgg ccatcagtcc cgtcgtcgga tcaatatcga
      241 gcgtgatcgt gcccatcagg cgcgatgagc cgtccgtttg cgcgccgccg cgcccttcga
      301 gacgcggggt ggtcgtgcgc ggacgctgcg gcggtgcgtc ggcaggcggt ggcgggtcgc
      361 tgccatcgga aggcacgatg acgggcgcgg gcgcatagtc aaactcgccg ccttcttcgc
      421 ccgtgccgtc gagcggcgcg agttcatctt cgggcggggc gttgctttcg atggtgggcg
      481 cggttcccgt gatgaagagt tcggtgcgct tgcccgcagt aatgtcggcg acggcgagca
      541 ggccggtgcg cggatcgatt gaagcctctt cgaggccggg gggcatctgc cagtcgcccg
      601 cccattcggg atgcgcggag agcgcggact gcatgaagtc cgcccagatg ggcaacgcgg
      661 aatccgcgcc cgtcaggccg agcggcgaac cgtcgtcgaa gccgacatag acgacgcaga
      721 cgagttgcgg cgtgtagccc gcgaaccaac cgtcgcgcga cgttccggtc ttgcccgcga
      781 cgttgaactt gaacccgcgc ccgcgcaggc gcgccgccgt gccgcgattg acgacatcct
      841 tcatcatcga agtcatcagg taggcgactt cggggcgcat cacgtcgttc tgctgcgtgg
      901 cgggcgcggc gatggtcgcg ccgcgtccgg tcgtgacgcg attgatgccg ataggcgtgg
      961 tgcgcgtgcc gaggcgggca aaggtcgtat aggcggaggc gacttgcagc ggcgtcgctt
     1021 cgttcgttcc gagcgccatc gcgggatagg cgcgttgcgg cttgggcagc cccgcgcgcg
     1081 tggcgaggtt catcacgcgg ccaatcgtga cctccatcgc cacgtcaacc gtgatcacgt
     1141 tcttactatg cacgagagca tcgcgcaggg tgacgggagc gttggcgtaa gagtcgccga
     1201 agttgccggg cgagtattcc tgattgtcgt aggtgaaagt cttcggctcg tccatgtagg
     1261 tcgtcgccgc cgtgatgacg cgcgggacgg gatcgtaagc cgtgttgagc gcggtggcat
     1321 agacgaaagg cttgaagacc gaaccgggct ggcgcatggc atccgtcgcg cgattgagtt
     1381 gactgcgttc gtagtcgcgc ccgcccacca tcgccaccac ttcgcccgtc tgcgcattca
     1441 tcgccacgag cgacgcttgc agcgtgcccg gcgcaaccga attcttcccg cgcttcgcgt
     1501 aaattttatc gagcgcggcg agttgcttcg tcacggcggc gtaggccgcg cgctgcaatt
     1561 ccatgtcaat cgtcgtgtag atgcgcaggt gttcggcggt gcgggcatcg gcgaggatgt
     1621 ctgccaactg cgcctgcacg taatcgacga agtagggagc ttcggaactg tcgagcctgc
     1681 cgcgcgcggg cgcgactttc aactccgtcg ccttcgcacg cgccgcctct tcctccgtga
     1741 tcgctccggc ctcggccatc gaatcgagca cttgattgcg ccgcgccgtc gccgtctgta
     1801 gattgcgata agggttgtag cgattcgggc tgcggatgat cccggcgagg aaagccgctt
     1861 cgggcagggt cacgcctgtc acgtccttgt tgaaatagac ctgcgtggcc tgcccgaaac
     1921 cgttgatcga gaagccgccg tgctggccga gataagcctc gttgcagtag agcgcgaata
     1981 tctcctgctt gttgagacgc gtctccagaa tgatcgacat gtaggcttcg gcgagtttgc
     2041 gccgcaggct gtattcgggc gagagcaaca gatttttgac gagttgctgc gtgatgctcg
     2101 aaccgccctg tcgggcaatc ggcgagttcg ggttggcgtc gtagcggcgc acgagcgcgc
     2161 gcaggatgcc gcgtatgttg atgccgtagt gctcgaagaa agtgcggtct tccgtcaccg
     2221 tgatggcttt gacgaggtgc ggcgggatgt cttgaaaacc gatgatgcgc cgcttctgcc
     2281 ggtcgctgcc cgtcacgaaa ctgatctgtt caggctcgat gagcgcgtgc tcaagtttcg
     2341 ttttgctgtc gagatcgttg atcgccgtga tgcccttgcc gtttctggcg aaagagacgc
     2401 gcacgcgcgg aaagagccgc tgcccatcca cgatggcgtt ctgccccggc tcgatctcca
     2461 ccgccgcgcc gtttgagaca taacgcccgc gcgcttcgtc cgcctgtgcg gcgcgctcga
     2521 cgtagcccgc gcgctgcaaa cgcttgacca attcgtccgc cgatagcccc gcgcccacgt
     2581 gcaactcttt cggcgcggta tagagtccgg cggagcgcgt gaagatttcg ccgctgatca
     2641 ggcggtctac gcgctcggag aagatgtagt agtaatagcc gagaacgccg atggtgaacg
     2701 tggtgagaat caccagcgcg atcacgagcg gcgggcgaaa gatgcgtcgc cgccaacgcc
     2761 ggaagaagcc ttcgtccggc tcttcgtatc tgatgacacg cgccccgcgc cgcttggcgg
     2821 cgggcggcgg aacgtgatta ccgggcggat gacgaacggc cataaaagca gtgacaagtg
     2881 acgggtgacg ggttgaagaa aatccttcgc cccgttcgag tctcattaaa aactctaatc
     2941 tctatacgcg caactgtcca ccattgtttg acactccgct cgcgttttcg ctaacgtgac
     3001 ggcgcgcctg atggctgccc ctgttggatg ctcagtttga tctatgagtt cgacgcacca
     3061 cctcccccac ctgtcgaaaa gcgcgcgccg ccgcacgttg tacgcgctcg ccgcgctcgt
     3121 cgctttgatc gctatcggca ccatcggctt tcaactgctc gaaccgctgt cgctgctcga
     3181 cagcctctat ttgaccgtga tgaccgtggc gaccgtcggt tacggcgacg tgcatccggt
     3241 gtcgcgcggg gggcgcgcct tctccatcgg ctttatgctc gtcagcgtcg ctgcggtcgg
     3301 cttcctgctc tcgacggcca ttcaagctct cgtccaatcc gagatcgtcg cgacttacga
     3361 ccagcggcga cgctcacggg agataaacaa actgcgaaac catttcatca tctgcggcgc
     3421 gggtcgcgtc ggctcgcgca tcatacgcga actgcaacgc cgcgaagagc ctttcatcgt
     3481 tatcgaacgc gaagagcgaa acgtcgccag tctgatcgag gaggacgtgc tcgtcctcgt
     3541 gcgcgacgcg acgctcgaag agacgctgcg cgatgcgggc gtcgaacacg cgcgcggcct
     3601 cgccgcctgc ctgccggacg atgccgacaa cgtctatgtc gtgctcacgg cgcgcggctt
     3661 gaacaacgat ctgcacatcg tcgcgcgggc ggtcgaagaa caggccgagc cgaagttgat
     3721 ccgcgccggg gcgaatcgcg ttatcgcccc cgtgatcatc ggcggccagc gcatgttgca
     3781 ggcgttgacc aagcccgccg tttcagactt catcgattcc atcgcggcgg aagacctcga
     3841 cctcgatttc gaagaggtcg tcgtttcgcc cgcgtcggct tacgtcgggc ataaattgaa
     3901 gttcaccaac atccgctcgg aactcgatgt cgtcatcgtc gccctacgcc ggagcagcaa
     3961 cagcgagatg atcttcaatc cttcgggcga cgctctgatc gagtcgggcg acatgctcat
     4021 cgccatcggt cgcgccgact cgctcgccaa actgaaaacg caggcgcaga ggaaaagtta
     4081 gtttcaggtt tcaagttgaa gcccgttttg aatctcagac ctgaaacacg aagccaatga
     4141 aagttctagt caccggcggc agcggttatc tcggcatcca cgtgcgccgt ttcttcgagg
     4201 ccgacgattt ttcgcgccgc gtcggcttag acattctcga cccttacgac gcttcgcgcg
     4261 ctgccgacta cgacgccgtg attcacctcg ccgcgcacct cgacaaaaac ccggaggggg
     4321 cggcggattg ttttcgcacg aacgccgaag gcaccgccaa cctgctcaca cacatgcgcg
     4381 aaggcgcaac ctttatctac gcttccacga aggacgtgta cggcgcacac gctgacggct
     4441 acaaggaagt cccggaaagt tgtgcgacgg aatactgcgg gcagtcgccg ctcgaatggt
     4501 cgaagctcat cggcgagcgg tacgtcgaat actacgcggc ggcgcgcggg attcgcgcct
     4561 gcatctttcg cctgtcggcg gtttacgcgc gcccctccga gggcaacgag tcgaacttcg
     4621 tcacgcatta cgtcgaagcg gtcaaacgcg gctggccgct cagcctgccc ggcggaggtc
     4681 aacctgtgcg cgacatcctg cacgtggaag atttctcccg cgcctgccgc gctttcatgg
     4741 attccggcct cacacgcggc ctctataatc tcggcggcgg gcgtgcgcag gcgacaactc
     4801 tgagcgggat cgtcgagaca atcgggcgtc tgattcaact ccaacccgtc atcgccgaag
     4861 acgcgcgact gcccgcgccc gtgcccttca actacgtctc cgatctctca cgcgcccgcg
     4921 ccgaactcgg ctggcaaccg ctcatcaaca tcgaagaggg tttgcaaagt ctcctgtaaa
     4981 aatcttgaag cattagttcg gcgcacacgc gcaaagaagt gggatgccgg acatcacttt
     5041 cggcctgcgc cttcttcctt tgcctctctg cgcctttgcg tgagacattt gttttatcgt
     5101 attaaggttg cagtcaaccg gatcgtcgat gacgctgaaa cttcatacag aacagatcga
     5161 gcgcgtggtg gtgcgcggcg cgaattgggt gggcgacgcg gtgatgaccg tggcggcctt
     5221 gcgcgaactg cgccgcgtgc tgcccgccgc gcacatcacg ctcgccacgc gcccctgggc
     5281 gcaagacatc ttcgcgggcg caggtatcgt tgacgaactg ttattgattg acgacacccg
     5341 gcgcggcgtg cgcgccacgc ttcgccagat cgcccagtgg cgcgcacgcc gtttcgatct
     5401 cgcccttctc ttcccgaacg ccttcgagcc tgctttcatc gccgccgccg cacgcgtccc
     5461 cctccgcgtc ggttacgcca ccgacgggcg cgcctttctc ctcacgcacc cgctcgcggt
     5521 tcccgcgtgg cgcagcgagc agcacgaagt tttttactat ctcaacctcg tcttcggact
     5581 cgaacacacg ttgacgggcg aagcgcagtt tgaaaatcgc gagccgcact ttcaactaca
     5641 aacttcttcc gaacgacagg cggcggcgcg acaactgctc gaacgccacg gcgcgcttcc
     5701 ggatcgcccg ctcgtcgcgc tctgccccgg ctcgaccaac agccgcgcca agcgttggcc
     5761 tgccgaacgc tacgccgcgc tcgcagacca cttgatgggc aaggccaaag cgaacgtcgt
     5821 gctcatcggc gcgcgcgagg aactggatgt gacgcaggag gtcgtcgcgc ggatgcggcg
     5881 gcgtccggtc gtgctgacgg gcgagactga tctggctgag acaatcgctg tcctgagcgt
     5941 cgccgacctg ctcgtcacca acgacacagg accggcgcac atctccgccg cgctcgaacg
     6001 tcccacgctc gtcatcttcg gcgcgacgaa tccgctcacg acgcgcccct tctccccgac
     6061 cgccgacatc atccgtcacc cgcccgactg cgcgccctgc atgctgcgcg attgtcccat
     6121 cgatcatcgc tgcatgacgg ccatcacgcc ggaagaggtt ttcctgcgcg ccgtggagat
     6181 gctcgcgcag gggcaatgcc ggacgcacgc ggcgggcgac gcctcgacac aagaagtcgg
     6241 cgcggaggtg gcttgatgaa ac
//
LOCUS       DASWNS010000267        14806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_450882, whole genome shotgun sequence.
ACCESSION   DASWNS010000267 DASWNS010000000
VERSION     DASWNS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14806
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450882"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /gene="fabD"
                     /locus_tag="VGB76_09465"
     CDS             complement(<1..825)
                     /gene="fabD"
                     /locus_tag="VGB76_09465"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY0099169.1"
                     /translation="MSIAYIFPGQGSQFAGMGRELAEAFAPARAVFAEADDALGFSLS
                     QLCFEGAAEALQLTENTQPAILTVSIAALRAMEAEGYPAPDFVAGHSLGEYSALVAAG
                     ALSLADAVRTVRARGRYMQEAVPVGVGAMAAIVSANLESVETACREAALEGEVCSPAN
                     INSPKQIVIAGNTAAVERAMEILKERGAKRALKLAVSAPFHCALMQPAQDRLSAVLEQ
                     TEFADLRVPLVNNVDATALRSGEEARGSLVRQVSSPVRWLESVEFLASEGVRTFVEV"
     gene            complement(922..1911)
                     /locus_tag="VGB76_09470"
     CDS             complement(922..1911)
                     /locus_tag="VGB76_09470"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY0099170.1"
                     /translation="MANTSARILSTGRAYPEGVLTNADLEKMVETSDEWITSRTGIKE
                     RHKAADGEYTSQFAVRAAQQAIERARIDPSEIDLLICATVTPDQILPSTGCLIQAELG
                     AHRAAAFDLAAACSGFLYGLTLANQMVRGGQARYALVVGAEVLTRYVDYTDRSTCVIF
                     GDGAGAALLGPAPDSERGILGTRIRSDGRFAEQLYAPGGGTRLGISADNVTSGQHFFR
                     MKGNELFKVAVRSMADVSREVLGEAGYKGEDVKLFIPHQANQRITDAVANNLKVDSSI
                     VYSNIAMHGNTSSASIPIALDECVEQGRISEGDLVLLASFGGGVTWGAVLMRW"
     gene            complement(2017..3105)
                     /gene="plsX"
                     /locus_tag="VGB76_09475"
     CDS             complement(2017..3105)
                     /gene="plsX"
                     /locus_tag="VGB76_09475"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY0099171.1"
                     /translation="MSHERPKLTGKVVVDAMGSDRAPHPEIDGAMAAARDFNIGVILV
                     GQPERVQPELRRCGWRKQGDHGIELVEAAEVVGMDEPVATAIRRKKKSSLRIGAKLVA
                     EGLADGFVSAGNTGAAMATAKMVIGMLPGVDRPALAALIPTKAGKPVLLLDVGANSDC
                     KPHQMAQFAIMGDAYSRSVLGTNRPAVGLMSIGEEEAKGNELTKEAFPLLRELSSLNF
                     VGNVEGRDVFTGAVDVIVTDGFTGNVMLKLSEGLQDAVISLIKRELSASAITKAGAML
                     ARPAFQRLKKKLDYSEYGGAPLLGVQRLVVIGHGSSNARAIRNAVRIVKEFSEHGAGD
                     RIEEGIAETHARELQLSDVKFQMSGAGD"
     gene            complement(3158..3343)
                     /gene="rpmF"
                     /locus_tag="VGB76_09480"
     CDS             complement(3158..3343)
                     /gene="rpmF"
                     /locus_tag="VGB76_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY0099172.1"
                     /translation="MPNPKRRHSKARTGKRRAHDALRAPQTQTCPNCQEPRMPHRVCP
                     KCGHYKGREVVRVNTEV"
     gene            complement(3461..4000)
                     /locus_tag="VGB76_09485"
     CDS             complement(3461..4000)
                     /locus_tag="VGB76_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY0099173.1"
                     /translation="MRIEADKLKEASTPFAHVYAPDKLPLEDELMRLASEAKVEGRAS
                     KRHEQVRLQGNVKASIEVRCDRCLAPVVVPVDADFDVAYIPAKLEEVTPEPTELQADD
                     LNFAVYEGGELDIDELTREQLLLALPVRQLCREDCKGLCPTCGADLNTQECQCEQPEI
                     DPRWAALAALKKSKESSDR"
     gene            complement(4068..5336)
                     /locus_tag="VGB76_09490"
     CDS             complement(4068..5336)
                     /locus_tag="VGB76_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099174.1"
                     /translation="MRRSFQEIRCQRCGTANPLGEELCGRCGTRLMLVVEPSTLRFEE
                     EVVARGQNEGQLLERISLLENNLLRFAERLEKGFELLLKQSQIALSDHALLETLIALL
                     EETGIVDRGKLSELWRAVLAGEKADDREQSTLELLRASILEDYQGTERKVFAGLVGQG
                     LQLLADGKTMQARRELERAAALAPTHTTLNFIIGLQFFREGKFALARVYLERAHEAQP
                     DSTQVYLLLGIARADDGEVEQARKLLHASIEARGSSYAAHYALGRLFAAEDNWKDAHA
                     HFKGALKAKSCAEAHYVLSLTLARLGRLRTALRHAVKAVELDENYAAAFQSLGFIRQQ
                     LGESKSAQKAFATARALGANGGAAKAGTKKRAKNGEPDVSEEMLLHAFFGAGRHRAKR
                     LLTGGDKRLAESLREDALGQVGFGTLTPAR"
     gene            complement(5424..6278)
                     /locus_tag="VGB76_09495"
     CDS             complement(5424..6278)
                     /locus_tag="VGB76_09495"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: protein motif:HMM:NF004474.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY0099175.1"
                     /translation="MTETIGVVGAGTMGNGIAQVAARAGYRVIMRDVKDEFLERGLKS
                     IDRSLQRDVDKARLAEAEKGAIIERISATTDLSALKDASFVVEAVTENFAVKAEIFRA
                     LDELTQPEAILASNTSSISITKLGGATRRPDKVIGMHFMNPVPVMKLVEVIRGIATSD
                     ETYARTHALTEKLEKIPLECNDSPGFVSNRVLMPMINEAIFALHESVATRESIDGIMK
                     LGMNHPMGPLTLADFIGLDVCLAILNVLHEELGDPKYRPCPLLKRYVDAGWLGRKTGR
                     GFYNYSDK"
     gene            complement(6301..6828)
                     /locus_tag="VGB76_09500"
     CDS             complement(6301..6828)
                     /locus_tag="VGB76_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099176.1"
                     /translation="MTPEDHNKTLGICHLVYGGLMLLLMFAMFIFMWMMFSEIPSRPG
                     RPDAEMPAAFLAIFFAFMFVYSLIFFVPSLVAGYAMLKRKTWARMASIIASVFEVMSF
                     PLGTAVGIYSFWFMFSETGKSLYGGSSVAPAGVRPYALHDAPPQPASDWNAQTSFGRE
                     REYTPPTQPPAWRDE"
     gene            complement(6828..8018)
                     /locus_tag="VGB76_09505"
     CDS             complement(6828..8018)
                     /locus_tag="VGB76_09505"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY0099177.1"
                     /translation="MPDSTEAVIISAARTPTGKFQGALKDFSATDLGALVVRESVRRA
                     GVAPEDVDEVIMGCVVQAGVGQNPARQAALKGGIPFGVSAVTINKVCGSGLKSVMMAA
                     QEVKLGDAGIVVAGGMESMSNAPYLLPKAREGYRLGNGTLVDAMIHDGLWCAFENYHM
                     GNTGEVVAERYNVTRAEQDEYALNSHRKAAAAIKAGKFRDEILPVEIPNKKGAPVIFD
                     TDESVREDTSIEALTKLKPAFKKEGGTVTAGNAPGVNDGASAVVVTSLERARALGVEP
                     LARIAAQATSGIQPELVMMAPVEAIRKVLKKAGWSLNEVDLIELNEAFSVQAVAIMRE
                     LELDPAKVNVNGGAVALGHAIGQSGSRLLTTILYEMKRRGARRGLCALCLGGGNAVAM
                     AVER"
     gene            8277..9323
                     /locus_tag="VGB76_09510"
     CDS             8277..9323
                     /locus_tag="VGB76_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEY0099178.1"
                     /translation="MQALILAGGKGTRLRPLTVYTPKPIVPICNRSFLLYQIDTLRNA
                     GITDITLSLSYQPHKIEQLIGDGAEHGVKIKYTVEPQPMGTAGAYKFAEELIREPTVV
                     FNGDILTDLDLEAVIREHNERRAAATLVLAPVENPSAYGLVETEADGRVRRFLEKPKP
                     DEISVNTINAGTYVLEPRVLDAIPAGENYSFEYQLFPDLLRRGEPFYAHIPRATYWID
                     IGTSERYLQVHHDLLAGRVSHVNLAERQGDFESATAAEIDERSLIADGCIIKPGAQII
                     NSVLGQGCYVDERARIENSVVWSHARISAGAQISNAIIGRGCHVGREAQVGAGVVLGD
                     KTSLTDYTRTGGAL"
     gene            9320..10801
                     /locus_tag="VGB76_09515"
     CDS             9320..10801
                     /locus_tag="VGB76_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEY0099179.1"
                     /translation="MSGRMINERIPRTPILFGTDGWRAVIAHEFTFANLERVAQAYAD
                     FLNQPQQQGAQDRRTNEATPHVVVGYDRRFLSEKFAERAAEVLAGNGITVAMFGSDVP
                     TPLVSWAVRERGAAGGVVLTASHNPAEFNGFKIKAAWGGSATRETTAAVEALVDAQTP
                     RRSDLQAAARGGELDESIESYRAQIADYVDLEKLRGAEGRVVVDPMHGSGGRWVESFL
                     RGGTLAVETIRAERDTQFGGVNPEPIDRNLAPLRARVSEAGALVGLATDGDADRVGAV
                     NEQGETMTMHEVVPLMLLHLVRRRNMTGGVVRTFSQSVLTKRIAAAHNLKLHETPIGF
                     KYVADLMLREDILIGAEESGGVGVKGHIPERDGILNSLLFLEAIVAAGKPPSELVREM
                     HREFGEFYFGRRDLHIEVARGLNLIETLSVSPPEEIAGYTVHGYSHLDGMKLVFGDES
                     WLLFRQSGTEPVLRIYAEATSVEKMNALLDEGSAMALKAAARG"
     gene            10904..11737
                     /gene="mutM"
                     /locus_tag="VGB76_09520"
     CDS             10904..11737
                     /gene="mutM"
                     /locus_tag="VGB76_09520"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY0099180.1"
                     /translation="MPELPEVELVARALDSLVSSRRVVAARLLRAGLAPETPPAVFAR
                     RLRGRRIERVTRRGKHLLFEFEGEVILIAHLRMTGRFLLLPLEAELPKHTHALFYLDD
                     ERRLVFSDQRHFGMMKLCGAAEVSHTKELRLLAPEPFSGDFTPAYLGQIFARTRRTLK
                     ETLLDQTKVTGLGNIYAAEALFLARINPFRPAYEVSTRRLPRLHQAILDVFNESIAHG
                     STMNVDPENIDGSYYDGGFSGRWRVYDREGEPCLDCRAPIRRITHAGRSTYFCPRCQR
                     R"
     gene            complement(11864..14074)
                     /locus_tag="VGB76_09525"
     CDS             complement(11864..14074)
                     /locus_tag="VGB76_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0099181.1"
                     /translation="MRNRLKLFTLAFILLSFSTHALAQEKLLTIDDLFDPVKRVNFSG
                     STPNPRWLKDGRSYLVAGDPAKPGTPRLLRVNALTGDSAAFFDAAKMEAALNALPGMS
                     TEDARRLANRTNYQMNPAQTGALLNHANDLFYYEFGAERAWRLSNSPEEEVGETFSPD
                     GRMVSYVRGNNIYIVDLAAQKERALTSDGSPKILNGRLDWVYQEELYGRGNFEGYWWS
                     PDSNMIAYLRLDETPVHEFAVVDHIPVRQELEITPYPKAGDPNPIVKLGIVNASGGAT
                     RWVDTYKYLPTDLLITRVGWTPDSRRVIFHAQDREQKMLDLNLANATDGKTSLLFHEE
                     TKAWVEAKDNNPRWLKDGSFLWLSDRNGWQHLYHYAADGRLIRQVTDGKWEIRSVEGV
                     DETKGFIYFTGTEHSHIAPNAYRIKLDGTERTRLTQTEGSHRVDFNPTFTHYVHYWND
                     LNTPPQVRLFDANGKLVRVIAENRVTTLGQYKLGKAELMQVKTRDGFTMEAMMIRPPD
                     FDPAKKYPVMSFTYSGPQAPQVRNGWGGAGYLWHQMLAQKGYIVWICDNRTASNKGVE
                     SAWPVYRNFGELELRDLEDGVSWLKSQPYVDGSRIGIWGWSYGGFMTTYALTHSKSFK
                     IGIAGGSVTDWRDYDSIYTERYMGTPQNNPEGYRKASPLHNARDLSGKILLLHGLMDD
                     NVHVQNTIQFAYELQKANKPFQLMLYPKSRHGVTDPLLVKHMRQLMTDFIIQNL"
     gene            complement(14143..14406)
                     /locus_tag="VGB76_09530"
     CDS             complement(14143..14406)
                     /locus_tag="VGB76_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099182.1"
                     /translation="MSTTIDATGMSPVAATGTSGLGGHTRALSTLFSTEMWERFSYYG
                     MRAILLTLCVRHRGCDHAGRRLRLSVTYSGHQKNGSAFNLIGC"
BASE COUNT         2779 a         4676 c         4375 g         2976 t
ORIGIN      
        1 gacctctacg aacgtccgca cgccctcact cgccagaaac tcgaccgact ccagccagcg
       61 caccggcgaa gagacttgcc gcacgagcga cccgcgcgcc tcttcgccac tacggagcgc
      121 agttgcgtct acgttgttaa cgagcggcac gcgcagatcg gcaaactccg tctgttccaa
      181 aaccgcgctc aggcgatcct gcgcgggctg catcaacgcg caatgaaacg gcgcgctgac
      241 agccagcttg agcgcgcgct tcgccccgcg ctctttcaat atctccatcg cgcgttcgac
      301 cgccgcagtg ttccccgcga tcacgatctg cttcggcgag ttgatattcg cggggctgca
      361 aacttcgccc tcaagcgcgg cttcgcggca cgccgtctcg accgactcaa ggtttgcgct
      421 gacaatcgcc gccatcgcgc cgacgccgac cggcacggcc tcctgcatgt aacgaccgcg
      481 cgcacgcacc gtccgcaccg cgtctgcgag cgaaagcgcg cccgccgcga cgagcgcgga
      541 gtattcgccg aggctgtgcc cggcgacgaa atccggcgca gggtagcctt ccgcttccat
      601 cgcgcgcaaa gctgcaattg agacagtcaa gatggcaggc tgcgtgttct ccgtcaactg
      661 caacgcttca gccgcacctt cgaagcagag ttgcgagagc gaaaagccga gcgcgtcgtc
      721 ggcctcggcg aagaccgcgc gcgcaggcgc aaacgcttcg gccagttcac gccccatccc
      781 ggcgaactgc gacccctgcc cggggaaaat gtatgcgata ctcattagaa atccgggtca
      841 ggggaagagg taaggaataa aaggagaaag acaaatttct tccttttccc cttccccttt
      901 accctctccc ctgtcttcaa cttaccagcg catcaacacc gcgccccaag tgacgccgcc
      961 gccgaacgat gcgagcaaca cgagatcgcc ttcgctgatg cgtccctgct cgacgcactc
     1021 gtcgagcgca atcgggatcg aggcggaaga ggtgttgccg tgcatggcga tgttggaata
     1081 gacgatgctg ctatcgactt tcaggttatt cgccaccgcg tccgtgatgc gctggttggc
     1141 ttggtgcggg atgaacaatt tcacgtcctc gcccttgtag cccgcctctc ccaagacctc
     1201 gcgcgagaca tcggccatcg aacgcacggc caccttgaaa agttcgttcc ccttcatgcg
     1261 gaaaaagtgt tggccgctcg tcacgttgtc ggcggagatg ccgagacgcg tgcctccgcc
     1321 cggcgcgtag agttgttcgg cgaagcgtcc gtcggagcgt atgcgcgtgc cgaggatgcc
     1381 gcgctcgctg tccggcgcgg gtccgagcag agccgcgcct gcgccgtcgc cgaagatgac
     1441 gcaggtggag cgatccgtgt agtcaacgta acgcgtcagg acttctgcgc cgacgacgag
     1501 cgcgtaacgc gcctgcccgc cgcgaaccat ctggttggcg agcgtcaggc cgtagagaaa
     1561 accggagcag gcggcagcca gatcaaaagc ggcggcacga tgcgcgccga gttccgcttg
     1621 aatgaggcag ccggtggaag ggagaatttg atcgggcgtg acggtagcgc agatcagcag
     1681 gtcgatctcc gacggatcga tgcgcgcccg ctcgatggcc tgttgcgcgg cgcgcacggc
     1741 gaactgcgaa gtgtactcgc cgtcggcggc cttgtgccgc tccttgatgc cggtgcgcga
     1801 agtgatccac tcgtccgacg tttcgaccat cttttcgagg tcggcgttcg tcaggacgcc
     1861 ctccgggtac gcgcgtccgg tgctcaaaat gcgggcgctt gtgttcgcca tcttcaatcg
     1921 cctttccttc atcaaaagtg acaagtgacg agcaagaatc agaacaagtt cttagtttta
     1981 acttgtcact cgtcacttgt cactggttac agcttttcaa tctcccgcgc cgctcatctg
     2041 aaatttaaca tccgacaact ggagttcgcg cgcatgcgtc tccgcgatgc cctcctcgat
     2101 gcggtcgccc gcgccgtgct cggaaaattc tttgacgatg cgcacggcgt tgcgaatcgc
     2161 gcgtgcgttc gaactgccgt ggccgatgac gaccagacgt tggacgccga ggagcggcgc
     2221 gccgccgtat tccgagtagt ctaatttctt cttcaaacgt tgaaaggccg gacgcgccag
     2281 catcgctccg gccttcgtga tcgccgaggc agagagttcg cgtttgatga gcgagatcac
     2341 tgcgtcctgc aagccttcgg agagtttgag catcacgttg ccggtaaatc cgtctgtgac
     2401 gatcacgtca accgcgcccg tgaacacgtc gcgcccttcg acgttgccga caaagttcaa
     2461 actgctcaac tcgcgcagga gcggaaaggc ttccttcgtc agttcgtttc ccttcgcctc
     2521 ttcctccccg atgctcatca agccgaccgc cgggcggttc gtgccgagca cggagcgcga
     2581 gtaagcgtcg cccatgatag caaactgcgc catctggtgc ggcttgcaat ccgagtttgc
     2641 gcccacgtct agaagcaata ctggtttccc ggccttcgtc ggaatcaggg cggcgagcgc
     2701 gggacgatcc acgccgggca gcatcccgat caccatcttg gccgtcgcca tcgccgcgcc
     2761 cgtgttgccc gccgagacga aaccgtccgc gagtccttcg gccacgagtt ttgcgccgat
     2821 gcgcagagaa cttttcttct tgcgccggat ggcggtggcg acaggctcgt ccatgccgac
     2881 gacttccgcc gcttcgacga gttcgatgcc gtgatcgccc tgtttgcgcc agccgcaacg
     2941 cctcaattcc ggctgcaccc gctcgggctg gccgacgagg atgacgccga tattaaaatc
     3001 gcgcgccgcc gccattgcgc cgtcaatttc gggatgcggt gcgcggtcgc tgcccatcgc
     3061 atccaccacg accttgcctg tcagtttcgg gcgttcgtga ctcatcttca ggcgcgtgcc
     3121 gacatcaacg cagaccggtg cggaaattgt tcagacttta gacttcggtg ttgacccgaa
     3181 ccacttcacg gcctttgtag tggccgcact tcgggcagac gcgatggggc atgcgcggct
     3241 cctggcagtt cgggcaggtc tgcgtctgcg gtgcgcgtag cgcatcatgc gcgcgtctct
     3301 tccccgtgcg cgctttggaa tgtcttcgtt ttggattagg cataataatt ccttcgctag
     3361 tgacaagtaa tctttattaa gtgacaggtg acaggtgaca agtaagaatc aagacaagtt
     3421 aaagataaga ttgctgctct cacttgtcac tcgtcgcccg tcacctgtca ctgctttctt
     3481 tactcttttt caaagctgcg agcgcagccc agcgcgggtc tatctccggc tgctcgcact
     3541 ggcattcttg tgtgttcaaa tccgcgccgc aggtcgggca caagcctttg caatcctcgc
     3601 ggcataattg gcgcacgggc aaagccaaaa gcagttgctc gcgcgtcagt tcatcaatat
     3661 cgagttcgcc gccttcgtag acggcgaagt tcaaatcatc cgcctgtagt tcggtcggct
     3721 ccggcgtgac ctcttcgagt ttggccggaa tgtaggcgac atcgaaatcc gcgtccaccg
     3781 gaacgacgac gggcgcgagg cagcggtcgc agcgaacttc gatggaggct ttcacgttgc
     3841 cctgcaaacg aacctgctcg tgcctcttgc tggcgcgccc ctccaccttc gcttccgagg
     3901 ccagacgcat caattcgtct tcgagcggta gtttgtcggg cgcgtaaacg tgtgcgaacg
     3961 gcgtgctcgc ttctttcagt ttatctgcct caatccgcat ggctcgtccc cggtaggcgc
     4021 gcatagccga aaatatccgg cgcacgcaaa aggagcaatt atagcggtca gcgcgcgggt
     4081 gtcaacgtgc caaagccgac ttgcccaagc gcatcctcgc gtaaggattc ggcgagccgt
     4141 ttgtcgccgc ccgtcagcag gcgttttgcc ctgtgcctgc ccgccccgaa aaacgcgtgc
     4201 agcaacatct cttccgacac gtccggctcc ccatttttcg cgcgtttttt cgttcccgcc
     4261 ttcgccgcgc cgccattcgc gcccagagca cgcgcggtcg caaacgcttt ttgcgcagac
     4321 ttcgactcgc caagttgctg ccggatgaag ccgagcgact gaaacgctgc ggcgtagttt
     4381 tcgtccaact cgacggcctt gacggcgtgc ctgagcgcgg tgcgcagacg ccccagacgc
     4441 gcgagcgtca agctcaacac gtaatgggct tcggcgcacg acttcgcctt gagtgctccc
     4501 ttgaaatgcg cgtgcgcgtc cttccagtta tcttccgccg cgaacagccg cccgagcgca
     4561 taatgcgcgg cgtaggatga accgcgcgcc tcgattgaag catgcagcaa ctttctcgcc
     4621 tgttcgactt cgccgtcgtc ggcgcgggct atcccgagga gcaggtaaac ctgcgtgctg
     4681 tcgggttgcg cttcgtgcgc gcgttcgaga tagacgcgtg cgagtgcgaa cttgccttcg
     4741 cggaagaatt gcaggccgat gatgaaatta agcgtggtgt gcgtgggcgc gagcgcggcg
     4801 gcacgttcca actcgcgtct ggcctgcatc gtcttcccgt cggccagcag ttgcaaccct
     4861 tgccccacca acccggcgaa gactttccgc tccgtcccct gataatcttc gaggatgctc
     4921 gcacgcaaca attcgagcgt gctctgttcc ctgtcgtccg ccttctcacc ggccagcaca
     4981 gcccgccaca actcgctcaa ctttccccgg tcgacgattc ccgtctcttc cagcaacgcg
     5041 atcaaagttt cgagcagagc atgatccgac agggcgattt gcgattgctt caagagaagc
     5101 tcgaaaccct tttcgagccg ttcggcgaag cgcaggagat tgttctccaa cagcgaaata
     5161 cgctccaaca gttgcccttc gttctgcccg cgcgccacca cctcttcttc gaaacgcagc
     5221 gtcgaaggct cgaccacgag catcaggcgc gtcccgcaac gcccgcacaa ctcctcgccg
     5281 agcgggttcg ccgtaccgca tcgctggcaa cgtatctctt gaaaacttct tcgcatctct
     5341 aaaacagtga cgagtgacga gtgacaagat cacagacgag tttgagagac aagttcttgt
     5401 ttcaacttgt cacttgtcgc tcgtcacttg tcactgtagt tataaaatcc gcgccccgtt
     5461 ttacgaccga gccacccggc atcgacgtag cgtttgagca gcgggcaggg gcgatacttc
     5521 gggtcgccga gttcttcatg cagcacgttg agaatggcga ggcacacgtc gaggccgatg
     5581 aaatcggcga gcgtgagcgg ccccatcggg tgattcatgc cgagcttcat gatgccgtcg
     5641 atgctctcgc gcgtggcgac cgactcgtgg agcgcgaaga tcgcttcgtt gatcatcggc
     5701 atcaagacgc gattggagac gaagcccggt gagtcgttgc attcgagcgg aattttttcc
     5761 agtttctccg tcagggcgtg tgtgcgcgcg taggtttcgt cagaggtcgc aatgccacgt
     5821 atcacttcca cgagtttcat cacgggcacg ggattcataa agtgcatgcc gatcactttg
     5881 tcggggcgac gcgttgcccc accgagtttc gtgatcgaga tcgaagaggt gttggaggcg
     5941 aggatcgctt cgggttgcgt cagttcgtcg agcgcgcgaa agatttccgc tttgaccgcg
     6001 aaattctccg tcacggcttc gaccacgaag ctcgcatctt tcaatgcgct caagtcggtc
     6061 gtcgcgctga tgcgttcgat gatcgcgccc ttctccgctt cggcgaggcg cgctttgtct
     6121 acgtcgcgct gcaagctgcg gtcgatgctc ttcaagccgc gctccaaaaa ttcgtctttc
     6181 acgtcgcgca tgatgactct gtagcccgcc cgcgccgcca cctgcgcgat gccgttgccc
     6241 atcgtccctg cgccgacgac cccgattgtc tctgtcacga tttacctctc cccgaaaaat
     6301 ttattcatcg cgccaggcag gcggctgcgt gggcggcgtg tactcgcgct cgcgtccaaa
     6361 actcgtctgc gcgttccagt cggatgcggg ttgcggcggc gcatcgtgca gagcataagg
     6421 ccgcactccg gcgggcgcga cagaagaccc gccatagagc gacttgcccg tctcgctgaa
     6481 catgaaccag aaggaataga ttccgacggc tgtgccgagg ggaaaactca tcacctcgaa
     6541 caccgacgcg atgatgctcg ccattcgcgc ccacgtctta cgtttcagca tcgcatagcc
     6601 tgcgactaac gagggcacga aaaagatcag cgagtagacg aacatgaagg cgaagaagat
     6661 cgccagaaaa gcggcgggca tttccgcatc gggtctgccc ggacgcgaag gaatttcgct
     6721 gaacatcatc cacatgaaga tgaacatcgc gaacatcaac agcagcatca gcccgccgta
     6781 gacgagatga cagatgccca gagtcttgtt gtgatcttcc ggggtcatta tcgctccacc
     6841 gccatcgcca cggcgttgcc gccgccgaga cagagcgcgc acaatccacg ccgcgcgccg
     6901 cgtcgcttca tctcgtaaag aatcgtcgtc aacaggcgcg aacccgattg gccgatggcg
     6961 tggccgagcg cgaccgcgcc gccgttgaca ttcactttcg ccgggtctaa ttccagttcg
     7021 cgcatgatgg cgacggcctg caccgagaag gcttcgttga gttcgatcaa atctacttcg
     7081 ttcaaactcc acccggcctt cttcaagact ttgcggatcg cttcgaccgg agccatcatc
     7141 acgagttcgg gttggatgcc cgaagtggcc tgcgccgcga tgcgcgccag aggctccacg
     7201 ccgagcgcac gcgcgcgttc cagcgacgtg acgacgaccg ccgatgcgcc gtcgttgacg
     7261 cccggcgcgt tgcccgccgt caccgtgccg ccctccttct tgaacgcggg cttgagcttc
     7321 gtgagagctt cgatggaggt gtcttcgcgc acggattcgt ccgtgtcgaa aataaccggc
     7381 gcgcccttct tgttcggaat ctcgacgggg aggatctcgt ctctgaactt gcccgccttg
     7441 atcgccgccg cagccttgcg gtgtgagttg agcgcatact cgtcctgctc ggcgcgcgtc
     7501 acgttgtagc gttcggcgac gacctcgccc gtgttgccca tgtgataatt ttcaaacgcg
     7561 caccacagcc cgtcgtgaat catcgcatcg acgagcgtgc cgttgccgag gcgatagcct
     7621 tcgcgcgctt tgggcagaag atacggcgcg ttcgacatgg actccatccc gcccgcgacg
     7681 acgatcccgg cgtcgccgag tttcacttcc tgcgccgcca tcatcacgga cttcaaaccc
     7741 gaaccgcaga ccttgttgat cgtcaccgcc gagacgccga acgggatgcc gcccttaagc
     7801 gcggcctgcc gcgccggatt ctgcccgacg cccgcctgca cgacgcatcc catgatcact
     7861 tcgtccacat cctccggggc aacgcccgcg cgcctgacgc tctcgcgcac gacgagcgcg
     7921 ccgaggtctg tcgccgaaaa atctttcaac gcaccctgaa acttccccgt cggcgttcgc
     7981 gccgcgctga tgatgaccgc ttccgtactg tctggcatga tgactcctta aaaacagtga
     8041 caagtgacga gtgacaagtg acgagttaaa gaaagtgact tgcttgtcac ttgtcactcg
     8101 tcacttgtca ctgtagttca gattatacgt tgtcagcact aaagagagtc aaggcaggct
     8161 tgcgccgtgc gggcgttgtt caattggcaa ggggcgcgtg cgagtgttag actccgtcgc
     8221 gcccgaagcg ccaacaaacg ctaaactatt tcacgtctca tctatttcta aacttcatgc
     8281 aggcacttat tctagcaggc ggcaaaggta cacggctccg cccgctgacg gtctacacgc
     8341 cgaagcccat cgtccccatc tgcaaccgct cttttctgct ctaccagatc gacacgttgc
     8401 gcaacgccgg gatcacggac atcacgctct cgctctccta ccagccgcac aagatcgaac
     8461 aactcatcgg cgacggcgcg gagcatggcg tgaagatcaa gtacacggtc gagccgcagc
     8521 cgatgggcac ggcgggcgca tacaagttcg ccgaagagtt gatccgcgaa cccaccgtcg
     8581 tcttcaacgg cgacatcctg accgacctcg atctcgaagc cgtcatccgc gaacacaacg
     8641 aacgacgcgc ggcggccacg ctcgtgctcg cgcccgtgga gaatccgagc gcgtacgggc
     8701 tggtcgaaac cgaagcggac gggcgcgtgc gccgctttct ggagaagccg aagcccgacg
     8761 agatcagcgt caacacgatc aacgccggaa cttacgtgct cgaaccgcgc gtgctcgacg
     8821 ccatcccggc gggcgaaaac tattccttcg agtatcaact cttccccgac ctgctccggc
     8881 gcggcgaacc cttctacgcg cacatcccgc gcgcgactta ctggatcgac atcggcacgt
     8941 ccgaacgcta cctgcaagtg catcacgatc tgctggcggg tcgcgtgagc cacgtcaatc
     9001 tcgccgagcg gcagggcgat ttcgagtcgg ccaccgccgc tgagatagac gaacgctcgc
     9061 tcatcgcaga cggctgcatc atcaaaccgg gcgcgcagat catcaactcc gtgctggggc
     9121 aaggttgcta cgttgacgag cgtgcgcgga ttgaaaactc cgtcgtctgg tcgcacgcgc
     9181 gcatcagcgc gggcgcgcaa atctcgaacg ccatcatcgg gcgcggctgc cacgtcgggc
     9241 gcgaggcgca ggtcggcgcg ggcgtcgtgc tcggagacaa gacctctctc accgattaca
     9301 cgcggacggg cggcgcgtta tgagcgggcg catgatcaac gaacgcatcc cccgcacgcc
     9361 gatccttttc ggcacggacg gctggcgcgc agtgatcgcg cacgagttca ccttcgccaa
     9421 cctcgaacgc gtggcgcaag cctacgcaga cttcctcaac cagccgcagc agcaaggcgc
     9481 acaggacagg aggacgaacg aagcgacgcc gcacgtcgtc gtcggttacg accgccgttt
     9541 cctgtcggag aagtttgccg aacgcgcggc ggaagttttg gccggtaacg gaatcacggt
     9601 cgccatgttc gggagcgacg tgccgacgcc gctcgtctct tgggcggtgc gtgagcgcgg
     9661 ggcggcgggc ggcgtggtgc tcacggcctc gcacaaccct gccgagttca acggcttcaa
     9721 gatcaaggcc gcgtggggcg gcagcgcgac gcgcgaaacg accgctgccg tcgaagcgct
     9781 cgtcgatgcg cagacgccgc gccgcagcga cttgcaagcg gcggcgcgcg gcggcgaact
     9841 ggacgaatca attgaaagct accgcgcgca gatcgcagat tacgtcgatc tcgaaaaatt
     9901 gcgtggcgcg gaggggcgcg tcgtcgtcga tccgatgcac gggtcgggcg gtcgttgggt
     9961 ggagagtttt ttgcgtggcg gcacgctcgc cgtcgagacg attcgcgccg aacgcgacac
    10021 gcagtttggc ggcgtcaatc ccgaaccgat tgaccgcaac ctcgcgccct tgagagcgcg
    10081 cgtgagcgag gcgggtgcgc tcgtcggctt agcgacggac ggcgacgcgg acagggtcgg
    10141 cgcggtcaac gaacagggcg agacgatgac gatgcacgaa gtcgtcccgc tcatgctgct
    10201 gcatctcgtg cgccgcagaa atatgacggg cggcgtggtg cgcaccttct cccaatcggt
    10261 gctgacgaag cgcatcgctg ccgcgcataa tctgaaactt cacgaaacgc ccatcggctt
    10321 caagtacgtc gcagacctca tgctgcgcga agacattttg atcggggcgg aggaatcggg
    10381 cggcgtcggc gtcaaaggcc atatcccgga gcgcgacggc atccttaatt cgctgctgtt
    10441 tctcgaagcc atcgtggcgg cgggcaagcc gccttcggag ttggtgcggg agatgcacag
    10501 ggagtttggc gagttctatt tcgggcggcg cgacctgcac atcgaagtgg cgcgcgggct
    10561 gaacctgatc gagacgctct ccgtctcgcc gcccgaagag atcgccggtt acaccgtcca
    10621 cggctactcg catctggacg ggatgaaact cgtcttcggc gacgaatcgt ggctgctctt
    10681 tcgccagtcc ggcacggaac ccgtcctgcg catctacgcc gaagccacgt cggtcgaaaa
    10741 gatgaacgcg ctgcttgacg aaggcagtgc gatggcacta aaagctgcgg ccagaggtta
    10801 gaggccggag gtcagtcttg ctctgcccta acggcaaatg cggcgacgat ccgcagacgc
    10861 tttttcctaa cctctaaact caaatctccg acctctcttc cttatgcctg agcttcccga
    10921 agtcgaactc gtcgcacgcg cgctcgacag cctcgtcagt tcgcgtcgcg tcgtcgccgc
    10981 cagacttttg cgcgcggggc ttgcgcccga aacgccgccc gccgtgtttg cccgacgctt
    11041 gcgcgggcgt cgcatcgagc gcgtcacgcg tcgcggcaag catctgctct tcgagtttga
    11101 aggcgaagtg atcttaatcg cgcacctgcg catgacggga agattcttgc tcctgccgct
    11161 cgaagccgaa ctgcccaaac acacgcacgc actcttctat ctcgacgacg aacgcaggct
    11221 cgtcttctcc gatcaacgcc acttcgggat gatgaaactc tgcggcgcgg cggaagtctc
    11281 gcacacgaaa gaactgcgcc tgctcgcgcc cgaacccttc tccggcgact tcacgcccgc
    11341 ctacctcggc cagattttcg cacgcacgcg ccgcaccctg aaagagactc tgctcgatca
    11401 aacgaaggtc acgggtctgg gcaacatcta cgccgcagag gctctgttcc tcgcgcgcat
    11461 caatcccttt cgtcccgctt acgaagtttc cacgcgccgc ctgccgcgcc tccatcaagc
    11521 catcctcgac gtgttcaacg aatccatcgc gcacggttcg acgatgaacg tcgatcccga
    11581 aaacatcgac ggcagctatt acgacggcgg cttcagcgga cgctggcgcg tctacgaccg
    11641 cgaaggcgaa ccatgcctcg actgccgcgc cccgattcga cgcatcacgc acgccggacg
    11701 ctcaacctat ttctgcccgc gctgccagcg gagataagac aggggaaagg taaggggtaa
    11761 agaggaaagg ttggagacaa gaaaaagcgg gacgcagcac ttgatcgctg cgtcccgctt
    11821 cctgtcttaa ccttcgcgtc ttccgctctg ccctttttcc gtcttacaag ttctggatga
    11881 tgaagtcggt catcaactgt cgcatgtgtt tgacgagcag cgggtctgtg acgccgtggc
    11941 gcgacttggg atagagcatg agttgaaacg gcttgttcgc cttctgtagt tcgtaggcga
    12001 actggatggt gttctggacg tgaacgttgt cgtccatgag gccgtgcagg agcaggattt
    12061 tcccgcttag gtcgcgcgcg ttgtggagcg gcgaagcctt gcggtagcct tccgggttgt
    12121 tctgcggcgt gcccatgtaa cgctccgtgt agatgctgtc gtaatcgcgc cagtccgtga
    12181 cgctgccccc ggcgatgccg atcttgaagc tcttgctgtg cgtgagcgcg taagtggtca
    12241 tgaagccgcc gtagctccag ccccagatgc cgatgcgcga accgtcaacg tagggctgcg
    12301 acttcaacca cgagacgccg tcttccagat cgcgcagttc caactcgccg aagttgcgat
    12361 agaccggcca cgccgattcc acgcccttgt tgctcgccgt gcggttgtcg caaatccaga
    12421 cgatgtagcc cttctgcgcg agcatctgat gccagaggta gcccgcgccg ccccaaccgt
    12481 tgcgcacctg cggagcctga ggcccgctgt aggtgaaaga catcacaggg tatttcttcg
    12541 ccgggtcgaa gtccggcggg cgaatcatca tcgcttccat cgtgaagccg tcgcgcgtct
    12601 tgacctgcat gagttcggcc ttgccgagtt tgtactggcc gagggtcgtc accctgttct
    12661 ccgcgatgac gcggacgagt ttgccgttcg catcgaagag acgcacctgc ggcggcgtgt
    12721 tgaggtcgtt ccagtagtgt acgtagtgcg tgaaggtcgg gttgaaatct acgcggtgcg
    12781 agccttcggt ctgcgtcagg cgcgtgcgct cggttccgtc cagcttgatg cggtaggcgt
    12841 tgggcgcgat gtggctgtgc tccgtgcctg tgaaatagat gaagcccttc gtttcgtcca
    12901 cgccttccac ggaacgaatc tcccacttgc cgtcggtcac ctgccggatc agcctgccgt
    12961 ccgccgcgta gtggtagaga tgctgccagc cgttgcggtc gctcagccag aggaacgagc
    13021 cgtctttcaa ccagcgcggg ttattgtctt tcgcctcaac ccacgctttc gtctcttcgt
    13081 ggaagaggag actggtcttg ccgtccgtgg cgttggcgag attgagatcg agcatcttct
    13141 gttcgcggtc ctgcgcgtgg aagatgaccc tgcggctgtc gggcgtccag ccgacgcgcg
    13201 tgatcaaaag gtcggtcggg agatacttgt aggtatcaac ccaacgcgtc gcgccgccgg
    13261 aggcgttgac gatgccgagt ttgacgatgg ggttcgggtc gcccgccttc gggtagggcg
    13321 tgatctccaa ctcctgacgg acggggatgt gatccacgac cgcaaactca tgcaccggag
    13381 tttcgtccag acgcagatag gcgatcatgt tggaatcggg actccaccag tagccctcga
    13441 aatttccgcg cccgtagagt tcctcctgat agacccaatc gagccgcccg ttcaggattt
    13501 tgggcgaacc gtcgctcgtc agtgcgcgct ccttctgcgc agcgagatcg acgatgtaga
    13561 tgttgttgcc gcgcacgtag gaaaccatgc gcccgtccgg gctgaacgtc tccccgacct
    13621 cttcttcggg gctgttggac agacgccacg cgcgctccgc gccgaactcg taataaaaga
    13681 gatcgttggc gtgattgagg agcgcgcccg tctgcgcggg gttcatctgg tagttggtgc
    13741 ggttggcgag gcggcgcgcg tcctctgtag acatgccggg gagcgcgttg agcgcggcct
    13801 ccattttcgc cgcgtcgaaa aaggctgcgg agtcgcccgt cagggcgttg acgcgcagga
    13861 ggcgcggcgt gccgggcttg gccgggtcgc ccgcgacgag gtaactccgc ccgtctttga
    13921 gccagcgcgg gttcggcgtc gagccgctga agttgacccg tttaacgggg tcgaagagat
    13981 cgtcgatggt cagcagtttc tcctgcgcga gcgcgtgggt ggagaagctg agaaggataa
    14041 aagcgagtgt gaaaagttta agccgattac gcactgtgag attctcctga gatagctatt
    14101 agccgttagc tggtagcggt tagctttttg tctgcttaac agctaacagc cgattaagtt
    14161 gaacgcggag ccattttttt gatgaccgga ataagtaaca ctaagacgaa ggcggcggcc
    14221 agcgtgatcg cagccgcgat gccgaacaca tagagtaaga aggatggcgc gcatgccgta
    14281 gtagctgaaa cgctcccaca tctctgtgga gaagagcgtg gagagggcgc gcgtgtggcc
    14341 gcccagaccg ctcgtgccag tcgccgcaac cggactcatc cccgtcgcgt ctatagttgt
    14401 actcatctca ggctcttacc tcaactcagt ctgcttcggt ctaaattcgc cggggcgcgg
    14461 cgcgatttcc tgtgctgctt tggtgcgggg aggattttct gtctattttg gatgcagccg
    14521 acattcagcc aaaaaccaac gccaactgtc aagaagattc agtttgatac gcgaccggga
    14581 ggtaaacggt ttcacccgca acgacaagtt tctcgcccga ctttgacttc gcgccccaac
    14641 ttatattgac gggaaggttg ttgttcgggt aggatgcccg ctcttatgaa gacgcctcgc
    14701 cttctttgac aatccgacca aggggaatca ccttcttctt ccaccaccta aggagagatt
    14761 tttgcatgtc aggttccttc agatttccac gcttacgcac attcgc
//
LOCUS       DASWNS010000268         5093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_450969, whole genome shotgun sequence.
ACCESSION   DASWNS010000268 DASWNS010000000
VERSION     DASWNS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450969"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1717
                     /locus_tag="VGB76_09535"
     CDS             <1..1717
                     /locus_tag="VGB76_09535"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEY0099183.1"
                     /translation="SARAGDGKGVVHVATDGESYGHHFKFGDRCLAYGLEVEAAARGF
                     RVTNYGEFLAGHAPEMEVEIKAGADGEGTAWSCAHGVGRWTRDCSCHASAPEGWNQKW
                     RAPLRAALNYARDEAARVFAEQGAELFQDPWRARDHYVELLCDPRRDRAEFLARQAGR
                     RLSEVQQKRALTLLELQRSSLLMFTSCGWFFNDISGIETVQVLKYAARVMDFMSELEA
                     KSPRAGFLELLAGAHSNIATHGNGADIFRESVETARVTPQRIAASLAISSLVDKSEQD
                     GEIAGHAFSLTNFRQEQHGRLALATGRLMLETVSTRKRLDFAVAAMHVGDIDFYCVLK
                     EFPGEAAFAEATEKLWSQFRTGSVPMLLRLMQEDFGPADFGLEHLLPAGRQRIYEIVF
                     GRMVGRFSRQYEYLYEDNRRNIEMLQGVGFHLPEELRTAAEYTITRRLEREIRKFEEQ
                     NDRAAFHEALKIANEVARLGYRLDRTSMRRKFEKLIVTTVRACTREPQAENQETALAL
                     IDLANKLSLHANLERAQEIVYEAVQSDGSLGAQLEALALKLDLAPSLLHESSTASETT
                     LAVAS"
     gene            1769..4015
                     /gene="glgB"
                     /locus_tag="VGB76_09540"
     CDS             1769..4015
                     /gene="glgB"
                     /locus_tag="VGB76_09540"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY0099184.1"
                     /translation="MLEINNPSVPTGELARLFSFVHHDPHSILGAHPTPQGVIVRAYR
                     PEATSIELLIEDEPPRPMSRTHPAGLFEFLVADRAQTFPYRLRVSYADGNVFTQRDPY
                     AFLPTLGNFDEHLFAEGRHWRLYEKLGAHVRRIGSVEGVAFAVWAPNAQGVSVVGNFN
                     NWDGRLHQMRRLGVSGIWELFIPDLKAGALYKYEMHGHGRPPFLKADPYALQAEVPPA
                     TSSIVFEPAYQFTDGDWIERRAQRDHLRQPLSIYEVHLGSWRRVREEGNRSLSYRETA
                     PALADYLQEMNFTHVEFLPLKGHPYGGSWGYQVANYYAPTARFGTPDDFRYLVDYLHG
                     RGLGVIMDWVPAHFPKDSFALGRFDGSALYEHLDPRKGEHPDWGTYIFNYGRNEVRNF
                     LIANALFWIRECHVDGLRVDAVASMLYLDYSREDGRWLPNRFGGRENLEAIDFLRELN
                     ETVHREHPGVLMIAEESTSWPKVSHPVSEGGLGYDFKWNMGWMHDTLQYFGKDALYRR
                     FHHNNLTFGFIYAWSENFILPFSHDEVVHMKGSMLTKMQGTREQKFANLRALYAYMWA
                     HPGKKLLFMGGELGQWREWNDDQSLDWHLLDEPAHRGVQRLLGDLNRLYLEEPALWEA
                     DVTPAGFEWIDANSATENIVAFIRYAPTAKRRLVCVCNFSASLRTGYRLALPSAGSYR
                     VILNTDAGVYDGSDSVRLDAIEATADPLHLRPYSALIDLPPLSTIWLEVPRAEESMEA
                     GAGGVPEI"
     gene            4052..>5093
                     /locus_tag="VGB76_09545"
     CDS             4052..>5093
                     /locus_tag="VGB76_09545"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY0099185.1"
                     /translation="MGDVKTIGVLTGGGDAPGLNPAIKGLVYRASEHDIKVVGLYDGW
                     RSLLNPAGDVIALDRNVVRRWDRDGGTNLGSSRTNPFKTANGAGERVDRSHEVEENIA
                     RLKLDAVVACGGEDTLGVAARLHARGVRVVGIPKTIDKDLAGTDYTLGFDTALRNVTE
                     VIERSRTPAGSHGWVQVIEVMGRHAGHLALWSGVAGGADLILIPEHPFRYEKIYTHLK
                     ERLGDTSSTAAARRERTQPRYSVIVVAEGAHPADGEPVTVDDRHDAFGHARLGGIAEV
                     LARQIKENTPYDARAAMLGHPQRGGMPSPIDRIMGMMFGARAADALAEGQLGKLVSAR
                     GIAPACELSLVEI"
BASE COUNT         1001 a         1580 c         1577 g          935 t
ORIGIN      
        1 aagcgcacgc gccggagacg gtaagggcgt cgtccacgta gcgaccgacg gcgaatccta
       61 cgggcatcat ttcaaattcg gcgaccgctg tttggcttac gggctggaag tcgaagcggc
      121 ggcgcgcggc tttcgtgtga cgaactacgg ggagtttctc gcaggccacg cgccggagat
      181 ggaggtcgag atcaaggcgg gcgcggacgg cgaagggacg gcctggagtt gcgcgcacgg
      241 cgtcgggcgc tggacgcgcg attgcagttg tcatgcgagc gcgcccgaag gctggaatca
      301 aaagtggcgt gcgccgttgc gcgccgcctt aaactacgcc cgcgacgagg ccgcaagagt
      361 cttcgccgaa cagggcgcgg aactctttca agacccgtgg cgcgcgcgcg accattacgt
      421 cgaactactg tgcgacccgc gacgcgaccg ggcagagttt ttggcgcgac aggcggggcg
      481 tcgcctgtcg gaagttcaac agaagcgcgc gctcacgttg ctcgaattgc agcgcagttc
      541 gctgctgatg ttcacgagtt gcggttggtt cttcaacgac atctccggga tagaaaccgt
      601 gcaggtgttg aagtatgcgg cgcgcgtgat ggatttcatg tccgagttgg aagccaaaag
      661 cccccgcgcg ggatttctcg aactgctcgc cggagcgcac agcaacatcg ccacgcacgg
      721 aaacggcgcg gacatctttc gtgagtcggt tgagacggcg cgcgtcacgc cgcagcgcat
      781 cgcggcgagc ctcgccatct caagcctcgt ggacaaaagc gagcaggacg gagagatcgc
      841 cgggcatgct ttctcgctca cgaatttccg gcaggaacag cacgggcgtc tcgcgctcgc
      901 caccgggcgg ctcatgctgg aaaccgtctc cacgcgcaaa cgtctggact tcgccgttgc
      961 cgccatgcac gtcggcgaca tagatttcta ctgcgtgttg aaagagtttc cgggcgaagc
     1021 ggctttcgcc gaagcgacgg aaaagctctg gtcgcaattt cgcaccgggt cggtccccat
     1081 gctgctcagg ctcatgcagg aagattttgg tccggcggac ttcggcctcg aacatctcct
     1141 gcccgcaggc cgccagcgta tttacgagat cgtgttcggg cggatggtcg ggcgtttctc
     1201 cagacagtat gaatatcttt atgaagataa ccggcgcaat atcgagatgc tgcaaggcgt
     1261 gggatttcac ctgcccgaag aattgcgcac cgccgccgag tacacgatca cgcgtcgcct
     1321 tgaacgcgag atacgcaagt ttgaagagca gaacgaccgc gcggcctttc atgaagcgtt
     1381 gaagatcgca aacgaagtgg cgcggctcgg ttatcgtctc gaccgcacga gcatgcgccg
     1441 caagtttgag aagttgatcg tgacgacggt gcgcgcctgc acccgcgaac cgcaggcgga
     1501 aaatcaggaa accgcgctcg ccctgattga tctggcgaac aaattgagcc tccatgcgaa
     1561 tctcgaacgc gcgcaggaga ttgtctatga ggctgtgcag agcgacggct cgctcggcgc
     1621 acagttggaa gcgttggctc tcaaactcga tctggctccg agcctgttgc atgaaagttc
     1681 gacggcgagc gaaacgaccc tcgcagtggc gtcctgaatt gctccgtgcc agtgcgttag
     1741 actgaaaccg gggcagtact gaaaacttat gttagagatt aacaacccga gcgtccccac
     1801 gggcgagttg gcgcggctct tctcttttgt tcatcacgac ccgcactcca tcctcggcgc
     1861 acacccgacg ccgcaaggcg tcatcgtgcg cgcttacagg ccggaggcca cgagcatcga
     1921 gttgctcatc gaagatgaac cgccgcgccc gatgagccgg acgcacccgg cgggactctt
     1981 cgagttttta gtggcagaca gagcgcaaac tttcccctat cgcctgcgcg tctcttacgc
     2041 ggatggcaac gtcttcacgc agcgcgaccc ttacgccttc ctccccacgc tcggcaattt
     2101 cgacgagcac ctcttcgcgg aggggcgaca ctggaggctc tacgaaaaac tcggcgcgca
     2161 cgtgcgccgc atcggcagcg tggaaggcgt cgcgttcgcc gtgtgggcgc cgaacgcgca
     2221 gggcgtgagc gtcgtcggca atttcaacaa ttgggacggg cggctccatc agatgcgacg
     2281 cctcggcgtc tccggcatct gggaactgtt catccctgac ctcaaagccg gtgcgctcta
     2341 caaatacgag atgcacgggc atggccgccc gccgtttctc aaggccgacc cttacgcgct
     2401 gcaagcggaa gtgccgcccg ccacctcctc catcgtcttc gaacctgcat atcaattcac
     2461 ggacggagac tggatcgagc ggcgcgcgca acgcgaccac ttgcggcaac cgctctcgat
     2521 ctacgaagtc catctcggat cgtggcgacg cgtgcgcgag gaagggaacc gttcgctcag
     2581 ctaccgcgaa accgcgcccg cgctcgcaga ctatttgcag gagatgaact tcacgcacgt
     2641 cgagtttctg cccttgaaag gtcatcctta cggcggctcg tggggttatc aagtggcgaa
     2701 ctattacgcg ccgaccgcgc gcttcggcac gcccgacgat tttcgttatc tcgtagacta
     2761 cctacacggg cgcggcctcg gcgtgatcat ggattgggtt cccgcacact tcccgaaaga
     2821 ctctttcgcc ctcggtcgtt tcgacggcag cgcgctctac gagcacctcg acccgcgcaa
     2881 aggcgagcac cccgattggg gaacctacat cttcaactac gggcgcaacg aagtgcgcaa
     2941 cttcctcatc gccaacgcgc tcttctggat cagggaatgc cacgttgacg gcctgcgcgt
     3001 agatgccgtc gcttcgatgc tctatctcga ttacagccgc gaagacgggc gttggctgcc
     3061 gaacaggttc ggcgggcgcg agaatctgga agccattgat tttctgcgag agttgaacga
     3121 gacggttcac cgcgagcatc ccggcgtgtt gatgatcgcg gaagagtcaa cgtcctggcc
     3181 gaaggtcagc catccggtgt ccgagggcgg cctcggctac gatttcaaat ggaacatggg
     3241 ctggatgcac gatacgctgc aatacttcgg caaggacgcg ctctatcgtc gctttcatca
     3301 caacaacctg accttcggtt tcatctacgc ctggagcgag aatttcatac tccctttttc
     3361 gcatgatgaa gtcgtgcaca tgaagggttc gatgctgacg aagatgcagg gcacgcgcga
     3421 gcagaagttt gcgaatctgc gcgccctcta cgcctacatg tgggcgcatc cgggcaagaa
     3481 acttctcttc atgggcggcg aactcggaca atggcgcgaa tggaacgacg accagagttt
     3541 ggactggcac ctgctcgatg aacccgcgca tcgcggcgtg cagaggctcc tcggcgacct
     3601 caaccgtctc tacctcgaag aacccgcgct ctgggaggcg gacgttacgc ccgcgggctt
     3661 tgaatggatc gatgccaaca gcgcgacgga aaatatcgtt gccttcattc gctacgcgcc
     3721 cactgcaaaa cggcgtctcg tctgcgtctg caatttctcc gcctcgttgc gaacgggcta
     3781 tcgtctcgct ctgccgtcgg caggcagtta ccgcgtcatc ctgaacaccg atgcaggggt
     3841 ttacgacggc agcgattctg tccggctcga tgccatcgaa gcgacggcag acccgctgca
     3901 cctgcgcccc tactccgcgc tcattgatct gcccccgctc tctaccatct ggcttgaagt
     3961 gccgcgcgcg gaggagagta tggaagcggg cgcgggcggc gtgccggaaa tttaagttgt
     4021 cggaaacatc agaggagcaa aatatacttt catgggtgat gtgaaaacca tcggtgtgtt
     4081 gacgggcggc ggcgatgcgc ccggcctcaa ccccgcaatc aaggggctgg tctatcgcgc
     4141 ctcagaacac gacatcaagg tcgtcggcct gtatgacggc tggcgcagtc tactcaatcc
     4201 ggcgggcgat gtgattgcgc tagaccgcaa tgtcgtgcgt cgctgggaca gggacggcgg
     4261 cacgaatctc ggctcatccc gcaccaatcc cttcaagacc gcgaacggcg cgggcgaacg
     4321 cgtcgaccgc tcgcatgaag ttgaagagaa catcgcgcga ctcaaactcg acgccgtcgt
     4381 cgcctgcggc ggcgaagaca cacttggcgt ggcggcgcgc ctgcacgcgc ggggcgtgcg
     4441 tgtggtgggc atcccaaaga cgattgacaa agacctggcc ggaaccgatt acacgctcgg
     4501 cttcgacacc gcgctccgca acgtgacgga agtgatcgaa cgctcgcgca cgcccgcagg
     4561 ctcgcacggc tgggtgcagg tgatcgaagt gatgggacgc cacgcaggcc acctcgcgct
     4621 ctggtcgggc gtggcgggcg gcgcggattt gatcttgatt cccgagcacc cgtttcgcta
     4681 cgaaaagatt tacacgcact tgaaagagcg tctgggcgac acctcttcaa ccgctgcggc
     4741 tcgacgcgag cgcacgcagc cgcgctattc cgtcatcgtc gtggccgaag gcgcgcatcc
     4801 cgcagacggc gaacccgtga cggttgacga ccgccacgat gcgttcgggc acgcgcgtct
     4861 cggcggcatc gccgaagtgc tcgcgcgaca gatcaaggag aacacgccct acgatgcgcg
     4921 tgcggccatg ctcggccacc cgcagcgcgg cggcatgccc tcgccgattg atcgcatcat
     4981 gggaatgatg ttcggcgcac gcgccgccga cgcgctggcc gaagggcaac tcggcaagct
     5041 ggtttcggca cgcggcatcg cccccgcctg cgaactctcg ctcgtagaga tca
//
LOCUS       DASWNS010000269         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_320945, whole genome shotgun sequence.
ACCESSION   DASWNS010000269 DASWNS010000000
VERSION     DASWNS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320945"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VGB76_09550"
     CDS             complement(<1..325)
                     /locus_tag="VGB76_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099186.1"
                     /translation="MANEIISYFEMCQREGSSLQRGMNFNVGGTHSVILMSLRANAPY
                     HDQVLDGGFTLIYEGHDVPRSQSTPNPKLLDQPEQRPSGVLTENGKFHRAAQEFKKNL
                     RQPERV"
     gene            511..>3293
                     /locus_tag="VGB76_09555"
     CDS             511..>3293
                     /locus_tag="VGB76_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099187.1"
                     /translation="MKFDEKIEALVRDLPDAEGARAFYERLAAEHARVANKLVRDEGL
                     LSDALALAAWSPLLGTTLAQNPDYVSWLARERTDTHVRTTEELGESLARFALTHTQVE
                     PQVVLARFRRRELLRIYLRDVRRTNSIVETTEELSNLADTVLAYALDHARQELDNRYG
                     APLCTDERGRKTTASFCVVALGKLGSRELNYASDIDLLFLYSDNGETSGSGERGATTN
                     REYFNRLAERMTRLVGQQSGEGAAYRVDLRLRPHGRNGALSCSLAEALRYYRAQAQAW
                     ELQTLIRSRAAAGTASLYTRFAAEVRERIYSHAASVEDALSNVRLAKQKIDRQHADGS
                     RGYNVKLGRGGIREIEFIAQALQLAHGAQDIWLHAPHTLISLGRLADRRLITERERTE
                     LSDAYSFLRTLEHRLQMEHGLQTHSVPDDDVRRALVARRMNFTPPDALARFNRALEFH
                     TTRVRAVYERVFVETQTERAPAAPHAPGEGAPATVDKAAITNGAAATTTAAPVVATDA
                     TVAGENGATDVNGTDAATLVNATSHDATLHPPRLEAPVTGADADNAATLAAASVFATR
                     LIPDSIHLNLHPRASQATFESDANRRVARLLHDAARESLNQRRALTYVARVAASLDKS
                     SVNLELSFAHLRALVRLCGASEFFGEMLASNPALISALHTRVEVVRERDYRALLRAEI
                     DREGTFRDELIALRRAWSQLLVEAGARDVAGELSHADSNRVQTELAKASINAAYFIAR
                     REMARRYGNLAAGPRLSVLGLGRLASGGMDYGSDLDLVIVYDAQVTSPVAAFTREEAY
                     TRLVELMVAALSSLTRAGYLYRVDLRLRPDGKNGALVRSSASFVEYLRTRTDVWEWLA
                     YVKLCAVGGDLELGRDVERQARSVIHEAARGADAEELRGETRRVRERLERERGEASPA
                     ARGAIDIKFGAGG"
BASE COUNT          594 a         1103 c         1008 g          588 t
ORIGIN      
        1 gcaccctctc aggttgtcta agattcttct tgaattcttg agcagcgcga tgaaatttac
       61 cgttctccgt taacactccc gaaggacgct gctccggctg gtcgagtaac ttggggttag
      121 gagttgattg acttcgaggc acgtcatgcc cctcataaat caaagtgaat ccgccatcca
      181 acacttgatc atggtaaggc gcgttggcac gaagcgacat caaaataact gaatgcgtgc
      241 ctcccacgtt gaagttcata ccgcgttgga ggcttgatcc ctcacgctgg cacatttcaa
      301 agtaagagat aatttcattt gccataaccg caatgctcaa atgaacgcgc cgaatttaaa
      361 ttgatattgc taagttcaga atgaaggtct gcgcgaggag agattataac gcaggagtcg
      421 tggcctcaca gtttatattt cttcgcaagg tcaaatctca gtgccgctct gcaaggcggc
      481 gcgcttcgcg ttacaatcgt tggcgctttg ttgaagtttg acgaaaagat cgaggcgctc
      541 gtgcgcgacc tgccggacgc ggagggcgcg cgtgcgtttt atgagaggct cgcggcggag
      601 catgcgcgcg tggcgaacaa actcgtgcgc gacgaggggt tgctgtccga tgcgctcgcg
      661 ctcgcggcgt ggagtccgct gctggggacg acgctggcgc agaatcctga ttatgtgagt
      721 tggctggcgc gcgaacgcac ggacacgcac gtcaggacga cggaggagtt gggggaatcg
      781 ctcgcgcgtt tcgctctgac tcatacgcag gttgagccgc aggtcgtgct cgcgcgtttc
      841 cgtcggcgcg aactcttgcg catctatctg cgcgacgtgc gccgcaccaa ctcgattgtc
      901 gagacgacgg aagaactctc caatctcgcc gatacggtgc tcgcctacgc gctcgatcac
      961 gcgcgtcagg agttggacaa tcgctacggc gcgccgctct gcacggacga gcgcgggcgc
     1021 aagacgaccg ccagcttttg cgtcgtcgcg ctcggcaaac ttggttcccg cgaactcaac
     1081 tacgcctccg acatagacct cctcttcctc tactccgaca acggcgaaac ttccggctca
     1141 ggcgagcgcg gcgcaaccac caaccgcgaa tacttcaacc ggcttgccga acgcatgacg
     1201 cgcctcgtcg gccagcagtc gggcgagggc gcggcctatc gcgtggactt gcgcctgcgc
     1261 ccgcacgggc gcaacggcgc gctcagttgt tcgctcgccg aagccctgcg ttattaccgc
     1321 gcgcaggcgc aggcgtggga actgcaaacg ctgattcgtt cgcgcgccgc cgccggaacg
     1381 gcttcgctct acacgcgctt cgccgcagag gtgcgcgagc gcatctattc gcacgcggct
     1441 tccgtcgaag acgcgctctc gaacgtgcgt ctcgccaagc agaagatcga ccgccagcac
     1501 gccgacggct cgcgcggcta caacgtcaaa ctcggtcgcg gcggcatccg cgagatcgag
     1561 ttcatcgcgc aggcgctcca actcgcacac ggcgcgcagg acatctggct gcacgcgccg
     1621 cacacgctca tcagcctcgg tcgcctcgcc gaccgccgcc tcatcacaga gcgcgagcgc
     1681 accgaactct ccgacgctta cagcttcctg cgcacgctcg aacaccgcct gcaaatggag
     1741 cacggcctgc aaactcattc cgtccccgac gacgacgtgc gccgcgccct cgtcgcgcgc
     1801 cgcatgaatt tcaccccgcc cgacgcgctc gcacgcttca atcgcgcgct cgaattccac
     1861 accacgcgcg tccgcgccgt ctacgaacgc gtcttcgtag agactcagac ggagcgcgcg
     1921 cccgctgccc cgcacgcgcc cggcgagggt gcccccgcca ccgttgacaa ggccgctatc
     1981 accaacggcg cggctgcgac gacaacggct gcgcccgttg tcgcaaccga tgcaacggtc
     2041 gccggtgaaa acggcgcaac cgacgttaac ggcactgacg ccgcgacgct ggttaacgcg
     2101 acctcgcacg atgcgacgct tcacccgccg cgacttgaag cccccgtcac cggcgcagac
     2161 gccgacaacg ccgccacgct cgccgccgcc agcgtctttg cgacccgcct cattcccgat
     2221 agcattcatc tcaacctcca cccgcgcgcg tcgcaggcga cgtttgagag cgacgcgaac
     2281 aggcgtgtcg cgcgtctcct gcacgatgcg gcgcgcgaat cgctcaacca gcggcgcgcg
     2341 ctcacctacg tcgcacgcgt cgccgcctcg ctcgataaat cgagcgtcaa cctcgaactc
     2401 tcctttgcac acttgcgcgc gctcgtccgt ctctgcggcg cgtcggaatt cttcggcgag
     2461 atgcttgcga gcaaccccgc cctgatctcc gctctgcaca cgcgcgtcga agtcgtgcgc
     2521 gagcgcgact atcgtgcgct cttgcgcgca gagattgacc gggagggaac ttttcgtgat
     2581 gagttgatcg cgcttcgacg cgcgtggtcg cagttgctcg ttgaagcggg agcgcgcgac
     2641 gtggcgggcg aactctcaca cgccgattcc aaccgcgtgc agaccgaact ggcgaaggcg
     2701 agcatcaacg ccgcttactt catcgcgcgg cgcgagatgg cgcggcgtta cggaaatctc
     2761 gcagccggac cacgcctctc cgtgctcggc ctcggtcgcc tcgcgagcgg cggcatggat
     2821 tacggctccg atctcgatct cgtcatcgtc tacgacgcac aggtgacttc gcctgtcgcc
     2881 gctttcacgc gcgaagaggc ttacacgcgg ctcgtcgagt tgatggtggc cgcgctgtcg
     2941 agcctgacgc gcgcgggcta tctctatcgc gttgacctgc gcctccgccc ggacggaaaa
     3001 aacggcgcgc tcgtgcgcag ttccgcctct ttcgtcgaat acctgcgcac gcgcacggac
     3061 gtttgggaat ggctcgccta cgtgaaactg tgcgcggtgg gcggcgacct cgaactcggt
     3121 cgcgacgtag agcgacaggc gcgcagcgtg attcacgaag cggcgcgggg cgcggatgcg
     3181 gaggagttgc gcggggagac gcggcgcgtg cgtgagcgtc tggagcgcga gcgcggcgag
     3241 gcgagtccgg cggcgcgcgg cgcgattgat attaaattcg gcgcgggcgg cat
//
LOCUS       DASWNS010000270         7573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_219131, whole genome shotgun sequence.
ACCESSION   DASWNS010000270 DASWNS010000000
VERSION     DASWNS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219131"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..454
                     /locus_tag="VGB76_09560"
     CDS             161..454
                     /locus_tag="VGB76_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099188.1"
                     /translation="MQFVYATGQPRNMEFAITSRQDRVPSGATVYATLCGSKAVENLD
                     EHTFNAMSDEEFYTFRSLVQNARQGESAEARDGEYASAAQKGLNKIFGWLGLR"
     gene            complement(527..1384)
                     /locus_tag="VGB76_09565"
     CDS             complement(527..1384)
                     /locus_tag="VGB76_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099189.1"
                     /translation="MNSKTHPLQKIMRLAWPLILLCAASVTLFAQTRTESETPKVDPA
                     TAAALPAANDEKAEQIVKRAVEAQGGSAFLGVRTIIGRGFYTPYDKGISTLPQSFVDY
                     LVFPDRERTEFRGSRGRSIQTNFAGGGWLYESSTQSLTDMKPEHLEEFRLAMRTSTDN
                     LLRGWWRKEGARLTYLGRREAGLAKRNEAVRVTYPDGFTVDYEIGARDFLPAKTLYKR
                     KNAEGEEVSAEDRYASYTTIAGLTVPFVIDQFRGGEQTSRINYNSIEFNSPVPDTLFA
                     KPANIKAIK"
     gene            complement(1571..3850)
                     /locus_tag="VGB76_09570"
     CDS             complement(1571..3850)
                     /locus_tag="VGB76_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY0099190.1"
                     /translation="MVDLGTYKDRFAESGQRIFEYAMQESKRRDQNYIAVEHILHAFI
                     TEEAELFNSAMRDLSLDPFHIKMQIERRLDNNRFQHVGKGLRIAPETIDLFKRAMERS
                     RAQGRKTIESTDLFSALAQDGGLLSGVLRTLGADSEALVENVRARVRLREQDEEQYRK
                     KFELPPYLKHFGTSMNRLARMDKIPPTIGREREIQQMVEILCHRERANSPMLVGEPGV
                     GKTAVVEGLARLIELEPEKVPARLRNAHVVQLQMGGLVAGTMLRGMFEERIKGIIDEV
                     KERPDLILFIDEAHTIIGAGAALGTSSDAANMFKASLARGDLRIIGATTITEYKEYIG
                     EDEALARRFRMVKIDEPTLMQTREILLGLRPRLERNYSVTISDDAINMALEMAPKYIR
                     NLHLPDKAIGWLDTASVKVEINEPDASVVKPEHIIEVISQESHIPLDMISRDTTDRFQ
                     QMEIEVGKRVIGQKEAVKSVAQRLRLNKGPLKESHYKPDGVLLFLGPTGVGKTELAKA
                     VAEFMFGDEEKMVRIDMSEYGDGTVGVEKLIGMPRGIVGSERGGILTERLRENPYTVL
                     LLDEVEKASPTLMNLFLQAFDEGWLTDGRGKKVYLSDAIVIMTSNLGSENFKKYEKPL
                     GFGTKTLGDVRAVKGEVMKAAEQRFSPEFRNRIDEIVVFSPLTMDEVRQIAVIYLDKL
                     RRQMERQGKTVQVTERAIDSLTEKGFSPAYGARFLKRHIDERVKLPLTAMWKGASQFV
                     VDYEEGEITIKAIDAPSVN"
     gene            complement(4233..5870)
                     /gene="groL"
                     /locus_tag="VGB76_09575"
     CDS             complement(4233..5870)
                     /gene="groL"
                     /locus_tag="VGB76_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0099191.1"
                     /translation="MAAKDLKFSEEARGAMLKGVNTLANAVRVTLGPKGRNVVLGKKF
                     GSPVITKDGVTVAKEIELKDNYENMGAQMVREVATKTNDTAGDGTTTATVLAQAIFRG
                     GVKNVAAGANPMALQRGIQRATEAAVADLERQSKKVKSKAELANVASVSANNDREIGE
                     LIAEAMDKVGKDGVVTVEESRTLETELDIVEGMQFDRGYVSPYFATNPDRMEAVLEDP
                     LILIHEKKIAAMRELLPVLEQASSQGRALLIISEDVEGDALPTLVVNRLRGTIKVCAV
                     KAPAFGDRRKAILEDIAALTGARVITEDLGVKLENVTPDDLGTAKRVIVDKDSTTIVE
                     GAGAAKDIQGRVATIRRQIEETTSDYDREKLQERLAKLAGGVAVIRVGASTETEMKEK
                     KMRVDDAVNATRAAAQEGIVPGGGIALLRAARALDKLELKDADEQTGVRLLRRALEEP
                     LRRIAENAGVDGAIVIGRVEESKGKEGYNAATGKYEDLVAAGIIDPTKVVRTALQNAT
                     SVAALLLTSGAAITDAPEPKKAAGSPPMGGGEDYGDY"
     gene            complement(6013..6675)
                     /locus_tag="VGB76_09580"
     CDS             complement(6013..6675)
                     /locus_tag="VGB76_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY0099192.1"
                     /translation="MEDRMAEEARAGEADAQAAEGIRVTDRRRVSLDDSEEGVRVQEG
                     DAPSLKPKYVEELEARTRAAEQSAKDVQARFEQVRAELRRETDEIRQRLARTADERVM
                     REKAAFVNTLLPVLENLQRAMEAAAGGGSLEALMNGLNGTISGFESALASGGIEIISA
                     LGAPFDPELHEAVDTVEVEPARDGLVTAEYSRGYRMGGQLLRPARVQVGRANTAAQTA
                     TE"
     gene            complement(6812..7276)
                     /locus_tag="VGB76_09585"
     CDS             complement(6812..7276)
                     /locus_tag="VGB76_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0099193.1"
                     /translation="MGHKWNGWCDYGTLQGRMNRLFEETARRHDHLNEEREAELERAD
                     WIPASDVYETEAEFVIAIDLPGIERSTLEVNVDENRLTVRGERAIEAEGARRSGSVGG
                     GRPSGRFLSRFGPLPPTVDQKNIAAEYRDGVLSLRLPKRAEQKAGRVKIEIQ"
BASE COUNT         1353 a         2418 c         2111 g         1691 t
ORIGIN      
        1 ctgcgtccta aagtcgcccc tgaagctcga aaaacttact acataaatgc gtggtgacgt
       61 gcgcgtgcgc ccggtctatt gtgacttcac acaagggaag aggcgaagta gaccgaagca
      121 gggattgaaa attttattta cacgtttaag gagagagtcg atgcaattcg tttatgccac
      181 aggccagccg cgcaacatgg aattcgctat tacgagtcgt caggatcgcg tcccgtcggg
      241 cgcaaccgtc tacgccacac tctgcggcag caaggccgtc gagaacctgg acgagcacac
      301 cttcaacgcg atgtcggatg aagagtttta caccttccgc tcgctcgtgc agaacgcgcg
      361 acagggcgaa agcgcggaag cgcgcgacgg cgaatatgct tctgccgcgc agaagggctt
      421 gaataaaatc ttcggctggc tcgggctccg ctagacgctg cgcggggctc cgggcggcgg
      481 gcgcgaagct taagaaaggg caagggcttc gcgcccgcgc atagagttat ttgatggctt
      541 tgatgttcgc cggtttggcg aagagcgtgt cggggacggg gctgttgaac tcgattgagt
      601 tgtaattgat gcggctcgtc tgttcgccgc cgcgaaattg atcgatgacg aaaggcacgg
      661 tcaagcccgc gatggttgtg taactggcgt agcggtcttc cgccgacacc tcttcgcctt
      721 ccgcgttctt ccgtttgtag agagttttgg cgggcagaaa gtcgcgcgcg ccgatctcat
      781 aatccacagt gaagccgtcc gggtaagtca cccgcacggc ttcgttgcgt ttggcgagtc
      841 cggcttcgcg gcggcctaga taggtcagac gagcgccttc tttgcgccac cagccgcgca
      901 acagattgtc cgtgctcgtg cgcatcgcca gacgaaactc ttccagatgt tccggcttca
      961 tgtctgtgag cgattgcgtg gagctttcat agagccagcc gccgcccgcg aagttggtct
     1021 ggatgctgcg gccacggctg ccgcgaaact ccgtgcgctc gcggtcgggg aagactagat
     1081 agtcaacgaa agactgcggc agcgtggaga tgcctttatc gtagggcgtg taaaaaccgc
     1141 gcccgatgat cgtccgcacg ccgagaaacg cgctcccgcc ctgcgcttcc acagcgcgtt
     1201 taacgatctg ttcggccttc tcgtcgttcg cagccggtag cgcggctgcc gtcgccgggt
     1261 caactttcgg agtctcactc tccgtcctgg tttgagcgaa cagcgtgacg ctcgctgcgc
     1321 atagcaagat gagcggccac gcgagccgca tgattttttg taacgggtgt gtcttggagt
     1381 tcataaaact tgtcggcaac tatccggcca ttttctcccg tgccgggagg cacagaggag
     1441 tttatacaag aggaaggaca caagttaaag agggtttcag ctcatctata aatgctagaa
     1501 agctgaaacc ctccggttca caacgagctt gaccaatccg gcgcgctcgc acgcgactta
     1561 cgcgcttcgt ttagttgacg ctcggcgcat caatcgcctt gatcgtgatc tcgccttcct
     1621 cgtaatcaac cacgaactgc gacgcgcctt tccacatcgc cgtcaaaggc agtttgacac
     1681 gctcgtcaat atgccgtttg aggaagcgcg cgccgtaggc cgggctgaag cccttctccg
     1741 tgaggctgtc gatggcgcgt tccgtcacct gcacggtctt gccctgtcgc tccatctgcc
     1801 tgcgcagttt gtcgagatag atgacggcga tctgtctcac ctcgtccatc gtgagtggcg
     1861 agaagacgac gatctcgtca atgcggttgc gaaactccgg cgagaaacgc tgctccgcgg
     1921 ccttcatcac ttcgcccttc accgcgcgca cgtcgcccag agtcttcgtg ccgaagccga
     1981 gcggcttctc atacttcttg aaattctccg agccgaggtt cgaggtcatg atcacgatgg
     2041 cgtccgagag ataaactttc ttcccgcgcc cgtccgtcaa ccagccctcg tcgaaggctt
     2101 gcaggaagag gttcatcaac gtcggcgaag ccttctcaac ttcgtcgagc aggagcaccg
     2161 tgtaagggtt ctcgcgcagg cgttccgtca ggatgccgcc gcgctccgag ccgacgatgc
     2221 cgcgcggcat gccgatcagt ttctcgacgc cgaccgtgcc gtcgccatac tccgacatgt
     2281 ctatgcgcac catcttctct tcgtcgccaa acatgaactc ggccacggcc ttcgccagtt
     2341 ccgtcttacc cacgcccgtc ggtccgagga acagcagcac gccgtccggt ttgtagtgcg
     2401 actccttgag cggccccttg ttcagacgca gacgttgcgc gaccgacttg accgcctcct
     2461 tctgcccgat cacgcgcttg cccacctcga tctccatctg ctggaaacgg tcggtcgtgt
     2521 cgcgcgagat catgtcgagc ggaatgtgcg actcctgcga gataacctcg atgatgtgct
     2581 ccggtttgac gaccgacgca tccggctcgt tgatctcaac cttgaccgaa gccgtgtcga
     2641 gccagccgat ggccttatcg ggcaggtgca ggttgcgtat gtacttcgga gccatctcca
     2701 gagccatgtt gatcgcatcg tccgagatcg tgaccgaata gttccgctca agacgcgggc
     2761 gcaggccgag aagaatctcg cgcgtctgca tcagggtcgg ctcgtcaatc ttgaccatgc
     2821 ggaaacgacg cgccagagct tcgtcttcgc cgatgtattc tttgtactcg gtgatggtcg
     2881 tcgcgccgat gatgcgcaga tcgccgcgcg cgagcgaagc cttgaacatg ttggccgcgt
     2941 ccgaagacgt gccgagtgcc gcgcccgcgc cgatgatggt gtgcgcttcg tctatgaaga
     3001 ggatcaggtc ggggcgttct ttaacttcgt cgatgatgcc cttgatgcgt tcctcgaaca
     3061 tgccgcgcag catcgtgccc gcgacgagtc cgcccatctg cagttgaacg acgtgcgcgt
     3121 ttctgagacg cgcgggaact ttctccggtt cgagttcgat gaggcgcgcg agtccttcga
     3181 ccacagccgt cttgcccacg cccggttcgc cgacgagcat cggcgaattc gcgcgttcgc
     3241 ggtggcagag aatctccacc atctgctgaa tctcgcgctc gcgcccgatg gtcggaggaa
     3301 tcttgtccat gcgcgccaga cggttcatcg aagtgccgaa gtgtttcagg taaggcggca
     3361 gttcaaactt cttgcgatac tgttcttcgt cctgttcgcg caggcgcacg cgcgcacgca
     3421 cgttttcgac gagcgcttcg gaatccgcgc cgagcgtgcg gagcacgccg ctcaggagtc
     3481 cgccgtcctg cgcgagagcc gaaaagaggt ctgtggattc gatagtcttg cgtccctgcg
     3541 cacgcgaacg ctccatcgca cgcttgaaga gatcaatcgt ctccggcgcg atgcgcagac
     3601 ctttgccgac gtgctggaaa cggttgttgt ccagccgtcg ctcaatctgc atcttgatat
     3661 gaaacgggtc gagcgagagg tcgcgcatgg ccgagttaaa cagttcggcc tcctccgtga
     3721 tgaaagcgtg gagaatgtgt tcgacggcga tgtaattttg gtcacgacgc ttcgattcct
     3781 gcatcgcgta ctcaaaaatg cgctgcccac tctccgcaaa gcgatcttta tatgttccga
     3841 gatcaaccat tgtgtccctt caatcttgct aggtgcgacg cgtgcgcgcc gcgagtttgt
     3901 tgatcgccaa ttttcgcccg aatatattta cgcttgacga tccgtttcgg ttcttcgcgg
     3961 agaagatcaa tgcccaagtc tctccgttcg agcggctatt tttagatgcc tcgcgtcggc
     4021 ctcccgttgt gttacgggac gctgacgagc atatcacaat taactctgca attatctagc
     4081 cattgtttgt tgaaactctc gcgcacttgc tgcgaaagag aaagcgcgcc ccggcgcaga
     4141 ctaataaagc gttcgtttca acttcgcgga ttcccccgga aagtcaaaac gaacgctcca
     4201 ttttcttact gactgaaagc cgttcacgct ctttaatagt cgccgtaatc ttcaccgccg
     4261 cccatcggcg ggctaccggc ggccttcttc ggttcgggcg cgtccgtgat ggccgcacca
     4321 ctcgtcaaca gcagggcggc gaccgaggtg gcgttttgca aagccgtgcg caccaccttg
     4381 gtcggatcga tgatgcctgc ggccacgagg tcttcatatt ttccggtcgc cgcgttgtag
     4441 ccttccttgc ccttcgactc ttcgacccgg ccaatgacaa tcgcgccgtc tacgcccgcg
     4501 ttctccgcga tgcgacgcag aggctcttcg agcgcgcgac gaagcaggcg cacgcccgtc
     4561 tgctcgtctg cgtccttcaa ctccaactta tcgagcgcac gcgccgcgcg cagaagcgcg
     4621 atgccgccgc ccggcacgat gccttcctgc gccgccgcgc gcgtcgcgtt caccgcatcg
     4681 tcaacgcgca tcttcttctc tttcatctcc gtctcggtgg atgcgccgac gcgaatcacc
     4741 gccacgccgc ccgcaagttt cgccagacgc tcctgcaact tctcgcggtc gtaatccgaa
     4801 gtcgtctctt cgatctgtcg ccggatggtc gccacgcggc cttgtatatc tttcgccgcg
     4861 cccgcgcctt caacaatcgt cgtgctgtct ttatcgacga tgactcgctt ggccgtgccg
     4921 agatcgtccg gcgtcacgtt ctccaacttg acgccgaggt cttccgtgat gacgcgcgcg
     4981 cccgtcaagg ccgcgatgtc ttcgaggatg gccttgcgcc ggtcaccgaa agccggagct
     5041 ttgacggcgc agaccttgat cgtgccgcgc aggcgattga cgacgagcgt gggcagcgca
     5101 tcgccttcca cgtcttcgga gatgatcaaa agcgcgcgcc cctgactcga agcctgttcg
     5161 agcaccggca agagttcgcg catcgccgcg atcttctttt cgtgaatgag gatgagaggg
     5221 tcttccaaga ccgcttccat gcgatccggg ttggtggcaa agtaaggcga cacgtagccg
     5281 cgatcaaact gcatcccttc gacgatgtcc aactcggttt cgagcgtccg cgactcttcg
     5341 accgtaacga cgccatcctt gccgaccttg tccatcgctt ccgcgatcaa ctcgccgatc
     5401 tcccggtcgt tattggccga gacgctggcg acgttggcga gttcggcctt gcttttgacc
     5461 ttcttcgact gtcgctccag gtccgccacc gccgcttcgg tcgcgcgctg gatgccgcgc
     5521 tgcaaagcca tcgggttcgc gcccgccgcc acgtttttga cgccgccgcg aaagatcgcc
     5581 tgcgcgagca cggtggctgt ggtcgtgccg tcgcccgccg tgtcgttcgt cttcgtcgcc
     5641 acttcgcgca ccatctgcgc gcccatgttc tcgtagttat ctttgagttc gatctctttc
     5701 gccaccgtca cgccgtcttt cgtgatcacg ggcgagccga attttttgcc gagcacgacg
     5761 tttctgccct tcgggccaag cgtcacacgc accgcgttcg ccagcgtgtt cacgcccttc
     5821 aacatcgccc cgcgcgcctc ttcgctgaac ttcaaatctt ttgccgccac tgattgttct
     5881 ccttaaatat tctccgccgt ccgttgtccg acgccagttg ttttaactct tatcaactcg
     5941 aaattctcag cttcagttat tcgccgtgaa gcacaggcaa cgggcaactg actgctggca
     6001 agggacgaat cttcattccg tcgccgtctg cgcggcggtg ttcgcacgcc cgacctgcac
     6061 gcgcgcgggg cgcaggagtt ggccgcccat gcgatagccg cgactgtatt cggcggtcac
     6121 taatccatcg cgcgccggtt cgacttcaac cgtgtcaacc gcttcgtgga gttccgggtc
     6181 gaagggcgcg ccgagcgcgc tgatgatctc gattccgccg gacgcgagcg cgctctcaaa
     6241 tccgctgatc gtaccgttga ggccgttcat taacgcttcg agggagccgc cgcccgccgc
     6301 cgcttccatc gcgcgctgca aattctccaa cacgggcaag agcgtgttga cgaaagcggc
     6361 cttctcgcgc atcacgcgct cgtccgccgt gcgcgccaga cgctgcctga tctcgtccgt
     6421 ctcgcgtctg agttccgcgc gcacctgttc gaaacgcgcc tgcacgtcct tcgccgattg
     6481 ctccgcggcg cgcgtgcgcg cttcgagttc ttccacgtac ttcggtttca gactcggcgc
     6541 atcgccttcc tgcacgcgca caccctcctc cgaatcgtcg aggctcacgc gcctcctgtc
     6601 cgtcacacga atgccctcgg ctgcctgggc atccgcttct ccggcgcgcg cctcttcagc
     6661 cattctgtct tccatatttc gtctcttttt ctccctgcga aaagatacca taataagttc
     6721 agtgacaagt gacgagtgac aagttggaac aagttttatg atgacaagtt agaacaagtt
     6781 ttccgacttg tcacttgtca ccagtcgctc gtcactgtat ttcaatcttc acacgtcccg
     6841 ctttctgctc cgcgcgcttg ggcaggcgca gactcagcac gccgtccctg tattcagccg
     6901 cgatattttt ctgatcgacc gtcgggggca gaggcccgaa ccggctcagg aaacgcccgg
     6961 agggacgacc gccgccaacg ctgccgctcc ggcgcgcacc ctccgcttca atcgcgcgtt
     7021 cgccgcgcac cgtcaggcga ttctcgtcaa cattcacttc gagcgtgctg cgctcaatcc
     7081 ccggcagatc aatcgcgatg acaaactccg cttcggtttc atagacatcc gaggccggaa
     7141 tccaatccgc gcgctcaagc tccgcttcac gctcttcgtt caaatgatca tgccgccgcg
     7201 cagtctcctc gaacagacgg ttcatgcgcc cctgcaacgt cccgtaatca caccacccgt
     7261 tccacttgtg tcccactgta aaaacctccg attttttgga ggaatgaagg aagagggatg
     7321 agggatgaat aaaacagaag ccagccgacc ggcttgaatg ctttttctgc ttcatccctc
     7381 atccttccgc gctcatccct tagttacgct gttgccgccg ccgcctcgct cgcaaacttc
     7441 aagccgtcgc cgtccgccga gacgtggatg gtctggccgg gcgcgatctc gcctttcagc
     7501 agtttcgacg cgagcggatt ttgaatcaac gtctggatgg cgcgtttcaa gggacgcgcg
     7561 ccgtacatcg gat
//
LOCUS       DASWNS010000271        22893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_296037, whole genome shotgun sequence.
ACCESSION   DASWNS010000271 DASWNS010000000
VERSION     DASWNS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22893
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296037"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2294
                     /locus_tag="VGB76_09590"
     CDS             <1..2294
                     /locus_tag="VGB76_09590"
                     /inference="COORDINATES: protein motif:HMM:NF017502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sip1-related alpha-galactosidase"
                     /protein_id="HEY0099194.1"
                     /translation="QATQSNRPANSQGDGQTDLPLGIDASRGALQLKAGAAAVVSNMR
                     IRLRHGDGSTSTLYELEAAGQDAGSDKAGRFELLRYRLKPAAGAPGAPPVAAPQVQAT
                     LEFRRYLRPQVLVASLDYEGAPLAARDGVQLVMGLDNFARGMATKRFKLYWTAPVFIS
                     DHRLLSPANVLLLWQQMQGDAYHLMVPLAGGGMVSEVGVSEIEYRPEFRVSASSHDPR
                     HTPRRVPLFAYATSRDPYQLPRDTYQTAFAATEQYGQLRWQKSLPEPFGWLGWCSWNA
                     YQQTVNEEKILASARSLRDKRIPVGFVLIDDGWLTVKGQKLAAFEADPTKFPRGLGAT
                     ARALREQYHIPHVGVWHTFQGYWTGIDLDSPLARDYQLFRGREGNALPDPREGRGQKF
                     YADWYAKLKEWGFDFVKVDGQANNVKFTDGLMPLFVSAAGSQRNLQEAAQKHFNYDGG
                     QGTEGVGVINCMEMTLENAYNWRTSNVARNSDDYLPEVAHNFKDHIFQNAYNAYWMSN
                     FAYPDWDMFQSHDANAGYHAVARAVSGGPVYFTDEPGRERAELLRPLAFSDGRLLMLD
                     EPGQVTRDLLFTDVSLQPTPLKVFGRITRPGLSAGVVGAFNANKSAPHVRGALKPGDV
                     EGLAQGAEGVAIYRRSDGEASLQRGDNAVSPVTLAANGFELFALSSVRGGIAVFGLLD
                     KYLSPAAVVSVNREGLDVEVRLREPGDFGAWLESAPARVELDGRVLPASAYSYKRTLL
                     RVPRAAFGARSGETRLRIRLAAR"
     gene            complement(2389..6123)
                     /locus_tag="VGB76_09595"
     CDS             complement(2389..6123)
                     /locus_tag="VGB76_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0099195.1"
                     /translation="MPTVLAAVLCLALSSFAQESRGTLTGRVSDQTGAAIPNATVIVK
                     SQQTNIETTVTTGDEGNYTVTPLQPGRYTVSIEAPGFKRAVSDVVELFTATTSTFDVN
                     LEVGAVGETVTVNADAPLLEADTASRGQVIESTRISELPLVGRNPINLATLAPGVTFN
                     GNPQFNRPFDNGDNVNFSINGGLNRHNDFQLDGVPNNSITDANASRTVSSNNIAFIPS
                     AEATEEFKIQTNSYDAQFGRTGGGTINVSVKSGGSDYHGSIYEFARRYQLNANSFSNN
                     ARGLQTTGPCAGQEASPRFTRDQITCENLGGSKIDQYGFVFSGPLRIPRFGEGGDHFY
                     NGKDRTFFLFNAEKYRELSPGQGFSTVPTLLERRGDFSQSGVTIYDPLTTRPDPNNPG
                     QFIRDPFPGNVIPANRINLVGQNIVNGFSLPNTGTANQRFNNFYLSEGLGTDDFHSYV
                     ARVDHAFSDNQRMFVRFVNNRRDQFAFGGNNRVGLGIDTQGPLVRENYGVVIDSTTTI
                     SPTSILNVRLGFSRFLQAAFREASSPFDATSLGFTPAFSNARPISIVPRITFNDPAGG
                     IPEFGSRNPNSNITNTWSLPVYLTKIMGKHTFKLGGEYRHFQINQAGGSFNFGGGAFC
                     FTNFFTSRIAGNRSSGGAAFADLLLGAPSANSTFAGCNGNAPVTQLENVSPTTFQWGY
                     YAGYIQDDFKVTPRLTLNLGLRYDYEQPPVERYNRQNRGFGFGQASPLAAAVRNASAT
                     DCPSCANLNGGLLFTDVGGQPRGAFEPDRNNFQPRIGVAYQLGEKTVLRGGYGLYYFP
                     SAEYGGATGFSVISSYSGTLPGGGANQFIPRADALSNPFPSGLAQPTGSAAGLATQLG
                     GSINFTNPERRIPHIHQYSAGFQHELPFRTKLDISYVGSRSRDILTGDAQGGGARNLN
                     VLSAAQLARARQDPTYLTASVPNPFAGLIPGNATLNAPTIARQNLLVPFPQFGNPNTN
                     GQGVFIFGENVGRLDYNSLQASLEKRLSRGLVGVVSYTFSKNIGALNFLNDQDTEPAR
                     AVVDFDTPHVLVVSAVYQLPFGRGQRFFGSAGRAANLILGGFEYSMIGTYRSGRPINL
                     PGNADLIADPRISDPAFSNQNVPGTTATYFNNCVRRLNGTTTQFITNANGQRVQQACT
                     NPAFAERNTSNTLRTSPLRLGNLREPTATTFDMALNKTFAFTERFRLQLRLEAFNVFN
                     TPLFGSPDTSATSNTFGVLNPNNGQRNIARQVQLGFKFNF"
     gene            complement(6471..8849)
                     /locus_tag="VGB76_09600"
     CDS             complement(6471..8849)
                     /locus_tag="VGB76_09600"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY0099196.1"
                     /translation="MKHPGSTLCEIALVRLRALSCALLLALSCALSLFAVVQAVAAPA
                     SSAGAKRAADDAGFDLQPLAAQVRRLVEALEYLGEPFSPAERAALERASEQEDVALAL
                     KGIREVLDARSLFLVEISPESRVRVTRGRAEATLVEKGWRSFLVRVRNEAGVTAQLKA
                     ASPNALPVYARGQSATIPGGFSLDPRPTQRIKPSEVVNRWLDLSMFDKQPLEPRLSGL
                     LHEYRIIQLYSRDRGRREAQVSFNVGQGSQDIGFRNDVSVLFNCLPSKDVTLRVLDED
                     GTPTTASFHIRDRQERIYPAKYKRLAPDFSFHPQVYRADGERLRLPPGEYIIDVTRGP
                     EYLVQRETLRVAGGTKPQEFSVRLKRWINPAAGGWYSGDHHIHAAGCSHYEAPTQGVF
                     PEDIIRHIKGEGLNVGNVLTWGPCYYFQKQFFEAKLNRLSTPAHLMRYDVEVSGFPSS
                     HVGHLNLLGLKDQNYPGAKEIDDWPTWGLPILKWARAQGAIVGFAHSGWGLEVRTDKL
                     PNYEMPSFDGIGANEYIVDVTHDAVDFISAVDTPAVWELNIWYHTLNSGFRTRIAGET
                     DFPCIYGDRLGMGRSYVRLDGALDYRAWVEGLREGRSYVSDGRSHLLDFRVGDLHVGT
                     QKSELRLARAGRVRVNARVAALLDVKPNEALRARPHDQTPYWDIERARVANGREVPVE
                     LIVNGQPVARRNVVADGVAREVAFEVEIERSSWVALRILPSSHTNPVFVLVGDRPIRA
                     SRRSAEWCLKAVDQCWSQKAQKISPAERPTAEKAYEHARQVYRRIIGESALD"
     gene            complement(8858..11458)
                     /locus_tag="VGB76_09605"
     CDS             complement(8858..11458)
                     /locus_tag="VGB76_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEY0099197.1"
                     /translation="MNLKRERGSEEMTDASDGQAGFSRRHFLLTGATALGGVAALHLP
                     AFAQATQRTGAAGGAQQSLARRKVLHLIGHSHIDAAWLWPWRDGSNTVLTTMRSALDR
                     MRETPDFRYSHSSSAHYAWVERADPRMFAEIKERVREGRWELVGGWPVEPDCNIPSTE
                     SLVRHCLYGKNYFQRAFGVDIQIGFNPDSFGHPAGLPSILRRAGYRYYVFMRPQENEM
                     KLPLLFWWESPDGSRVLALRIRRGYSMAANRIPDAAENNFAPGFEHAAYFLGVGNHGG
                     SVTKEQIRQVTEMQKDTSLPELRWSTVGEFFKAVEASPAAATLPVVSSELQYHSRGVY
                     SAHGEIKQINRRAERWLGQAETISHAASLGVGQRYPAEEYAAAWGKVLFNQFHDLMAG
                     TALYTAYRDARDQLGAACDTAQTNMVEALEAIARRVDTRTVREGAVFAFNPLPWPRKC
                     LVEYHTERNPGSNPWVPVPAGVVPITHLETKDGQKVPLQFRPSDSMTQVYPRISAWVD
                     LPACGYKVFEVVHGTPPETAPYNNFFSVNDEGFGISSLKSADGKELLAAPIGLVVIGD
                     PSDTWGHGITRYRAEIGRPVFVSTQQIEDGAVTRVIRQRARWRNSEIVLDIAQFKAID
                     AIELRFHIDWQEREQILKLEVPTAFNAPRTFARVPGAVAERAPTGDEEPYQDWVAVEG
                     RIGGEQHTLGLVNNSTYSYDCKQGLLRTILIRSAPFARHDPHQVPHNDHGAWQDQGRQ
                     ERRFWLVRGKGDLAALELDRLAEEMQTPAEYVTDSAHEGTEPWERSFFRLSPGSVTLL
                     SLKRAESGEGTIVRVQERAGKATGFTLESAARGVNHRAQINPWEIKTLLLTGERGGKT
                     QVREVNLLER"
     gene            complement(11448..12032)
                     /locus_tag="VGB76_09610"
     CDS             complement(11448..12032)
                     /locus_tag="VGB76_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY0099198.1"
                     /translation="MEIKSGRIAEHFAAHQRVIEESITQLAEPAEEAARALVSALGAG
                     RKVIAFGNGGSAAQASHLVGELLGRFKATRQPFPAIALASDAGTVTCIANDFGYDMLF
                     ERQVRALVAPGDVAVGFTTSGRSENVRRGLEEARRRGAVTIAVVGSAGLVGGTADHVL
                     SVPSDQTAHVQEVHLMLLHVWCIYIDEALGQLES"
     gene            complement(12205..14319)
                     /locus_tag="VGB76_09615"
     CDS             complement(12205..14319)
                     /locus_tag="VGB76_09615"
                     /inference="COORDINATES: protein motif:HMM:NF028477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HEY0099199.1"
                     /translation="MPARTRVRAPVACCLVALFVVQTSAQALVPPPLLRPPAVPLVTH
                     DPYFSVWSTSDALADERTKHWTGAPHGMVGMARIDGKPHRFMGQSYSRLPAPMRQVRL
                     EVLPTRTLYEFEAEGVRLRLTFMSPLLPDDLEVLARPVTYLTWDAWAVDGRSHRVSLY
                     FDCTAEFAVNTTYEKVVWSRPKVGGLRVLTFGSQAQPVLERAGDDLRIDWGYLHLSVP
                     ASAAHTASDVIAWNQTARNSFAATGALPDSDDMRMPRMAREEMPVLATTFDLGEVAAA
                     PVSRHLMLAYDDLYSVEYFRRRLRPYWRRDGVEAGAMLQAAARDYEALKRRSEAFDAE
                     LMADLRRAGGEEYARLAALAFRQTIAAHKLAADWDGTPLFFSKENYSNGSIATVDVTY
                     PSAPFFLLFNPRLLEAQLKPVFDYAASPRWRFPFAPHDLGTYPLANGQTYGGRETSEV
                     DQMPVEESGNMLLLSAALARADGHARFADRYWPLLTRWAEYLRDKGMDPENQLSTDDF
                     AGHLAHNTNLSLKAILALGSYALLADLTGRKTEGRTYRRLAEEMAARWVKLADDGDHY
                     RLAFDKPGTWSQKYNLVWDRLLGLNLFPPQVARRELDFYRTKQNRYGLPLDSRESYTK
                     LDWIIWTATLSGSPEEFRSFVAPLYKFVNETPDRVPLTDWYGTLDGKQRGFQARSVVG
                     GVYIKMLEDAALWKKWSRRATR"
     gene            complement(14481..15482)
                     /locus_tag="VGB76_09620"
     CDS             complement(14481..15482)
                     /locus_tag="VGB76_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0099200.1"
                     /translation="MSQVTTEKGVGLRARVSEWGRVTEYVIVFAILIESVVFALIKPE
                     FFSVPNLINIALSIAITGILAVGMTMVILTGGIDLSVGSVVALAGVVAAMIAAAGGST
                     GSVILGSVAALGVGVGVGIFNGFVVAQFRVPPFVATLAMLTICRGLVFTITGGRSISN
                     LPAAFAFVGRERVLGLPVPVIIMFVVFAAGWVLLTRTVFGRYIYALGGNPRAAFHAGV
                     NTKLFTFLPYVLNGLLVGLAGLVLASRLGAGVPNAGLQYELDVIAATVVGGTSLTGGR
                     GSVIGTLWGAVFIGVLNNGLNLAGVDPYMQRIALGVVLLLAVLADQAGKTQGGQATR"
     gene            complement(15546..16505)
                     /locus_tag="VGB76_09625"
     CDS             complement(15546..16505)
                     /locus_tag="VGB76_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0099201.1"
                     /translation="MRSFVRLACLSLLLAAFSLMAGCQRTDQGGSASSSSSSTKRIGV
                     TLLTREHEFYRDLEAGLKDAADKQGYQLTVTSGDFDLAKQQSQIENFVVQKMDAIIVC
                     PADSKGIGPAIEQANNAGIPVFTADIAAQSGRVVSHVASDNLAGGRLAADFIARAIGG
                     AGSVGIIGQQEVQTGLDRENGFKEELAAKHPNVKVVAVLNGGGVRDRSLKAAEDMLQG
                     NSDIKAIFGINDDSALGALSAAEARGRSDLVIVGYDATPEAVKAIQRGSQLKADVAQQ
                     PRDLGAKTIEVIRTHFSGGQSPASISVPVKIVDAESLKASGGQ"
     gene            16818..17483
                     /locus_tag="VGB76_09630"
     CDS             16818..17483
                     /locus_tag="VGB76_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY0099202.1"
                     /translation="MKKRKIVLIHTSPAAIAPLMRFYNDAAPELEITNLLDDGLLRLL
                     ASKEHATVEERLAEMLEVGVRTYGAELVMVTCSSVTKGMVERLAPLFDLPLLKIDYSL
                     AREAVSARRRRIGLAATFPPTVVPTSQLISEAAEEAGAAVEIIQEVVPGAYDALLAGD
                     EEAHDELLCAGVERLAAGGVDVIVLAQISMARVLPRLEGRVAVPVLSSLHTSLSAIRA
                     ALK"
     gene            17489..18874
                     /gene="otnK"
                     /locus_tag="VGB76_09635"
     CDS             17489..18874
                     /gene="otnK"
                     /locus_tag="VGB76_09635"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: protein motif:HMM:NF043035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEY0099203.1"
                     /translation="MTSDSPTLSHDAPVHDIERLLFAAVADDDTGASDLAGMLAGQGV
                     RTLLVLDLPTPAQFAGWTRGYEAVVLAEGTRNLAPALAYERTRAAIRLLQTRCPRLFQ
                     LKYCSTFDSTAEGNIGAAIDAALDELAEEFTVALPALPVNGRTTYQGYHFVRQQLLSD
                     SSMRNHPLTPMTNPNLIELLGQQTKRRVGLAPYQVVREGAGVLKKHLDELRAGGFHVA
                     LVDCLDDEQLEQICRAVADLRLITGSSAFGMKLPAIWRERGLLERQAAPLPPPPRTES
                     DAGCLLVAGSCSAATLQQNAWAAEQGIRVRQIAPPRLLNGEPERAEIIAEARRELAAG
                     RHYLLTTSGAAAEVREAQAWGAARGLTVPALGESLAYALAELVLEVLEGQTLGGLILA
                     GGETSGAVCRRLELGAMRVGQNIEPGVPLCYSLGRFRLPLVLKSGNFGSPDFYGKATR
                     AVARSGEYLTI"
     gene            complement(18920..20188)
                     /locus_tag="VGB76_09640"
     CDS             complement(18920..20188)
                     /locus_tag="VGB76_09640"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEY0099204.1"
                     /translation="MNSVMMCGHARFPGAKDPRELPATGLIHNKKVIREETKQTKMKR
                     KGITILMLASAVVLLCGFQRRPKPVHAEAFGRVDGQQVELYTLTNRQGVEAKITNYGA
                     TVVSLKVPDREGRFADVLLGYDTLEGYRQSTFYVGPVIGRYANRIARGRFALNGKEYK
                     LAVNNGENHLHGGLKGFDKVVWKARPFASRDGAAVELTYLSKDGEEGYPGNLSVKVVY
                     TLTGKNELRMDYSATTDRDTVVNLTNHAYFNLAGQGNGDILKHHLTIDADRFLPADAK
                     SIPTGELRSVAGTPFDFRAAKAIGARIAQDDEQLKFGNGYDHTFVIRGRAGALRRAAT
                     VSEPSTGRVLEVWTTEPGMQLYTGNYLENTMIGKGGKAYGARQGFCLETQHFPDSPNK
                     PDFPTTVLRKGGHFNSTTIYKFFAGTGVAR"
     gene            20609..21601
                     /locus_tag="VGB76_09645"
     CDS             20609..21601
                     /locus_tag="VGB76_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEY0099205.1"
                     /translation="MKLSTRTALVSLTLLLTLSACQQNSTQTGGGGQASGQGGKKFAF
                     VTNNASDFWTIARRGTEKADAELADVTVEFRMPPDGTAADQKRIVDDLLAKGIDGIAI
                     SPVDPANQTQLINDTARQALVFTQDSDAPQSDRAAYVGTDNIAAGRQAGELIKEAIPE
                     GGRIMLFVGKSDARNAQERTQGIRESLQGSNVEIIDVRTDDTDRVRAKSNVSDTLVRY
                     PEVKALVGLWSYNGPAIVNALKESGKTGQVKIVAFDEEDETLAGVRDGSIYATVVQQP
                     FEFGYQAIKMMAQAAKGDRSGIPADKKVIVPTLIIKRDGIEEFQRKINGLRGRS"
     gene            21762..>22893
                     /locus_tag="VGB76_09650"
     CDS             21762..>22893
                     /locus_tag="VGB76_09650"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY0099206.1"
                     /translation="MTQILKMQGIEKRFGGVHALKGVDFDISAGEAVALVGENGAGKS
                     TLMKILGGVHQPDAGSISIDGAPAQIHSVADAIRHGVSFIHQELNVLDNLDVAANVFL
                     GREPVRGGFLNLIDRRRMYAETKVYLDRLGLHVPPDTPLSKLSIAQQQLVEIAKALSL
                     NARLLIMDEPTSSLTLTETDRLLALVKELKAQGVAVIYISHRLSEVEEISDRAVVLRD
                     GANAGSLARKEITHDQMVKMMVGRNLDNFYRQPTGAVSSGYLQVERLRTSRYPQQTIS
                     FSVGKGEILGLAGLVGSGRTEVAEAIFGVDGASDAQITLDSRPLRVRSPRDAIRHGIY
                     LIPEDRRHAGLITDMMIRENVTLPALERYASAGLVSFERERTA"
BASE COUNT         4127 a         7126 c         7514 g         4126 t
ORIGIN      
        1 cgcaggccac gcaatcaaac cgcccggcca actcgcaggg cgacggacag accgatctgc
       61 cgctcggcat tgacgccagc cggggcgcgc tccagttgaa agcaggggcg gcggcggtcg
      121 tctcgaacat gagaatccgc ctgcgtcacg gcgatggctc gacctctacg ctgtatgaac
      181 tcgaagccgc cggacaggac gcgggcagcg acaaggccgg acgctttgag ctactccgtt
      241 accgcctgaa gcctgccgcc ggtgcgccgg gcgcgcctcc ggtggccgcg ccgcaagtgc
      301 aggccacgct cgaatttcgt cgctacctgc gcccgcaagt tctcgtcgcc tcgctcgatt
      361 atgaaggcgc gcccctcgcc gcgcgcgatg gcgtgcaact cgtcatgggt ctggacaact
      421 tcgcgcgcgg gatggcgacc aagcgtttca agctctactg gactgcgccc gtcttcatct
      481 cagaccaccg cctgctgtcg cccgccaacg tcctgctcct ctggcagcag atgcagggcg
      541 acgcttatca cctgatggtg ccgctcgcgg gcggcgggat ggtgtccgag gtgggcgtct
      601 ccgagatcga gtaccgcccg gagtttcgcg tctcggcttc cagccacgac ccgcgccaca
      661 cgccccgccg cgtgccgctc ttcgcctacg ccacctcgcg cgatccttac caactgccgc
      721 gcgacaccta ccagaccgcc tttgcggcga ccgaacagta cggacaactg cgctggcaga
      781 agagcctgcc ggagccgttc ggctggctcg gctggtgctc ctggaatgcc taccagcaga
      841 ccgtgaacga agagaagatt ctggcgagcg cgcgctcgct cagagacaaa cggattccgg
      901 tcgggttcgt gttgattgac gacggctggc tcacggtcaa aggacaaaag ctcgccgcgt
      961 tcgaggccga cccgacgaag tttccgcgcg gtttgggagc gacggcgcgc gccctgcgcg
     1021 agcaatacca catcccgcac gtcggcgtct ggcacacgtt tcaaggctac tggacgggca
     1081 ttgacctcga ctccccgctc gcgcgcgact accagctttt ccgtggccgc gaaggcaacg
     1141 ccctgcccga cccgcgcgaa gggcgcggcc agaagttcta cgcggactgg tacgccaaat
     1201 tgaaggaatg gggcttcgac ttcgtcaagg ttgacgggca ggctaacaac gtcaaattca
     1261 cggacggcct gatgccgctc ttcgtctccg ccgcaggttc gcagcgcaac ctgcaagagg
     1321 cggcgcagaa acatttcaac tacgacggcg ggcaaggcac ggaaggcgtc ggcgtgatca
     1381 actgcatgga gatgactctg gagaacgcct acaactggcg cacctcgaac gtcgcgcgca
     1441 atagcgacga ctacctcccc gaagtcgcgc acaacttcaa agaccacatc ttccagaacg
     1501 cctacaacgc ctactggatg tcgaacttcg cctatcccga ttgggacatg ttccagtcgc
     1561 acgatgcgaa cgccgggtat cacgccgtcg cgcgcgccgt cagcggcggc cccgtctact
     1621 tcaccgatga accgggcaga gagcgcgccg aactactgcg cccgctggcc ttctccgacg
     1681 ggcggctcct gatgctcgac gagccgggac aggtgacgcg cgacctgctc tttacagacg
     1741 tgtccctgca acccacgccg ctcaaagtct tcgggcgcat cacgcgcccc ggcctttcag
     1801 ccggagtggt cggcgcgttc aacgccaaca agagcgcgcc acacgtccgg ggcgcgctca
     1861 aacccggcga cgtggaagga ctcgcgcagg gcgcggaggg cgtggcgatt taccggcgca
     1921 gcgacggcga ggcgagtttg cagagaggcg acaacgcggt ttcacccgtg acgctcgcgg
     1981 ccaacggctt cgagttgttc gcgctgtcgt ccgtgagagg tggcatcgcc gtgttcgggt
     2041 tgctggacaa gtatctaagc ccggcggcgg tcgtctccgt gaaccgcgag ggtcttgacg
     2101 ttgaagtacg cttgcgcgaa cccggcgact tcggcgcgtg gctggagagc gcgcccgcgc
     2161 gggtcgaact cgacggcagg gttttaccgg cctcggccta cagttacaaa cgcacactct
     2221 tgcgcgtacc gcgcgcggcg ttcggggcgc gctccggcga gacgcgactc cgcatacggc
     2281 tcgccgcccg gtgataagta aacgccggga cgctctctgc gccgaagata aaagggctac
     2341 ccccgatttc gggggcagcc cttttttaac ggacgtgcag gtcggacttt agaagttgaa
     2401 cttgaatccg agttgaacct gacgcgcgat gttgcgctgc ccgttgttag ggttcaagac
     2461 gccgaaggtg ttgctcgtcg cgctcgtgtc cgggctgccg aagagcggcg tgttgaagac
     2521 gttgaaggct tccagacgaa gctgtaaacg gaaacgctcg gtgaaggcga aggtcttgtt
     2581 cagagccatg tcgaaggtcg tcgccgtcgg ctcgcgcagg ttgcccaaac ggagcggaga
     2641 ggtgcgtagc gtgttgctcg tgttccgctc ggcgaaggcc gggttggtac aagcctgctg
     2701 cacgcgctgg ccgttcgcgt tggtgatgaa ctgcgtcgtc gtgccgttca ggcggcgcac
     2761 gcagttattg aaataggtgg cggtcgtgcc gggcacgttc tggttactga aggccggatc
     2821 gctgattcgc ggatcggcaa tcaggtcggc gttgcccggc aggttaatcg ggcgaccgga
     2881 gcgatacgtg ccgatcatgc tgtactcgaa gccgccgagg atcaggttcg cagcccgccc
     2941 ggcactgccg aagaaacgct ggccgcgccc gaacgggagt tggtagaccg cgctcacgac
     3001 gagcacgtgc ggagtgtcga aatccaccac cgcgcgcgcc ggttccgtgt cctgatcgtt
     3061 caggaagttg agcgcgccga tgttcttcga gaaggtgtag gagacgaccc cgaccagccc
     3121 acggctcaga cgcttttcga ggctcgcttg cagcgagttg taatcgaggc gtccgacgtt
     3181 ctcgccgaag atgaagacgc cctgcccgtt cgtgttcggg ttgccgaact gcgggaaagg
     3241 aacgagcaga ttctggcggg cgatggtcgg ggcgttcaaa gtcgcgttgc ccggaatcag
     3301 cccggcgaaa ggattgggca cgctggccgt caggtaggtc gggtcttggc gcgcgcgtgc
     3361 gagttgcgcc gccgacagga cgttcaggtt gcgcgccccg ccgccctgcg cgtcgcccgt
     3421 caggatgtcg cggctgcggc tgccgacgta ggagatgtcc agtttcgtgc ggaacggcag
     3481 ttcgtgctgg aagcctgcgg agtactggtg aatgtgcggg atgcggcgtt cggggttggt
     3541 gaagttgatg ctgccgccga gttgcgtcgc cagccctgcc gccgagcctg tgggctgcgc
     3601 cagaccgctc gggaacgggt tgctcaaagc atccgcgcgc gggatgaact ggtttgcacc
     3661 gccgcccggc aaagtgccgc tgtaggacga gatgacgctg aagccggtcg cgccgccgta
     3721 ttcggctgaa gggaaatagt agaggccgta gccgccgcgc agcacggtct tttcgccgag
     3781 ttgataggcg acgccgatgc gcggctggaa gttattgcgg tcgggttcaa acgccccgcg
     3841 cggttggccg cctacgtccg tgaagagcag gccgccgttc aggttggcgc acgaagggca
     3901 gtcggtcgca gaggcgttgc ggacagccgc cgccagcggg ctggcctgac cgaagccgaa
     3961 gccgcgattc tggcggttat aacgctcgac gggcggttgc tcgtagtcgt agcgcaagcc
     4021 taagttcagg gtcagtcgcg gcgtcacttt gaaatcgtcc tgaatgtagc ccgcgtagta
     4081 gccccactgg aaggtggtcg gcgagacgtt ttcgagttgg gtgacggggg cgttgccgtt
     4141 acaaccggcg aaagtggagt tggccgacgg cgcgccgagc agcaggtctg cgaaggccgc
     4201 gcccccggac gagcggttgc ccgcgatgcg cgaggtgaag aagttggtga aacagaaggc
     4261 gccgccgccg aagttgaacg agccgcccgc ctggttaatc tggaagtgcc ggtattcgcc
     4321 gcccaacttg aaggtgtgct tgcccatgat cttcgtcagg tagacgggca gactccacgt
     4381 gttcgtgatg ttcgagttgg ggttgcgtga gccgaattcg gggatgccgc cagccgggtc
     4441 gttgaaggtg atgcgcggca cgatggagat cgggcgggcg ttcgagaagg caggcgtgaa
     4501 gccgagcgag gtggcgtcga agggcgacga cgcttcgcgg aaagccgcct gtaggaagcg
     4561 cgagaagccg aggcgcacgt tcaggatgga ggtcggcgag atggtcgtgg tcgaatcgat
     4621 gaccacgccg tagttttcgc gcacgagcgg tccctgcgtg tcgatgccga ggccgacgcg
     4681 gttgttgcca ccgaaggcga actggtcgcg gcggttgttc acgaagcgga cgaacatgcg
     4741 ctggttgtcg ctgaaagcat gatcgacgcg tgcgacgtag gagtggaagt cgtccgtgcc
     4801 gagtccttcg ctgagataga agttattgaa cctctggttc gccgtgccgg tgttcggcag
     4861 cgaaaagccg ttgacgatgt tctgcccgac gaggttgatg cggttggccg ggatgacgtt
     4921 tccgggaaac ggatcgcgta tgaactggcc ggggttgttc ggatcggggc gcgtcgtcag
     4981 agggtcgtag atggtgacgc cggactgcga gaaatcgccc ctgcgctcca gcagcgtcgg
     5041 gacggtgctg aagccctgcc ccggactcag ttccctgtac ttctccgcgt tgaacaggaa
     5101 gaaggttctg tccttgccgt tgtagaaatg gtcgccgccc tcgccgaagc gcgggatgcg
     5161 gagtggtccg gagaagacga agccgtactg atcgatcttg ctgccgccga ggttctcgca
     5221 agtgatctgg tcgcgtgtga agcgcgggga agcctcctgt ccggcgcagg ggccggtcgt
     5281 ctgcaagccg cgcgcgttgt tggagaagga gttggcgttc aactggtagc ggcgcgcaaa
     5341 ctcgtagatc gagccgtggt aatcgctgcc gccggatttg acgctgacgt tgatcgtgcc
     5401 gccgcccgtg cgcccgaact gcgcgtcgta ggagttggtc tgaatcttga actcttccgt
     5461 cgcctccgcc gaagggatga aggcgatgtt gttgctgctc accgtgcggc tcgcgttggc
     5521 gtcggtgatg gagttgttcg gcacaccgtc gagttgaaaa tcgttgtggc ggttcagacc
     5581 gccgttgatc gagaaattga cgttgtcgcc gttatcgaac gggcggttga actgcgggtt
     5641 gccgttgaag gtcacgccgg gcgcgagcgt ggcgaggttg atcgggttgc gaccgacgag
     5701 gggcagttcg ctgatgcgcg tgctctcgat cacctgcccg cgcgaggccg tgtcggcttc
     5761 cagaagcggc gcgtcggcat tgaccgtcac cgtctcgccg accgcgccga cttcgaggtt
     5821 aacgtcgaag gtggaggtgg tggccgtaaa cagttcgacc acgtcgctga ccgcgcgctt
     5881 gaagcccgga gcctcgatgc tgacggtgta gcgccccggt tgcagggggg tcacggtgta
     5941 gttgccctcg tcgccggtgg tgacggtggt ttcgatattc gtctgctgac tcttgacgat
     6001 gacggtggcg ttcgggattg ccgcacccgt ctgatcgctc acccgtccgg tgagcgtgcc
     6061 gcgcgattct tgtgcgaaac tcgatagagc cagacacaag acagccgcta agacggtcgg
     6121 catacgccga caaaaaccag gggacataaa cttcctccta cagagagttc aaaggtgtga
     6181 ttattagctt gggaaccgat tgacgctgac agtggaaccg cccgagattc gaaaaggcta
     6241 tgttaaaacg gctgatcctc aggcacggga tgcgcgatac taacgacaac cgggacagga
     6301 aatcaagtaa ttttttcaac agtttagaaa ttagtccagc aggcgaacaa agttatcaac
     6361 gccgccggac gatttccgtc tccccgccgc acggcgggcg ggcacagccc ggcggaacaa
     6421 cacatagtca acaataagct taagcgtcgc tactggaaaa accgcaagag ctaatcgagc
     6481 gcgctctcgc cgattatgcg gcggtagacc tgccgcgcat gctcataagc cttttccgcc
     6541 gtcggtcgct cagccggaga aatcttttgc gccttttgac tccagcactg gtcaaccgct
     6601 ttcaggcacc actccgcgct gcggcgggag gcacggatgg gacggtcgcc caccagcacg
     6661 aacacagggt tcgtgtggga agacggaagg atgcgaagcg cgacccaact gctgcgctcg
     6721 atctcgacct caaaggctac ttcgcgcgcg acgccatccg cgacgacgtt cctgcgggct
     6781 acgggctggc cgttgacgat caactcgaca ggcacttcgc gcccgttcgc cacgcgcgca
     6841 cgctcgatgt cccagtaagg cgtctggtcg tgtggccgtg cgcgcagtgc ttcgttcggt
     6901 ttcacgtcga ggagcgcggc cactctggcg ttgacgcgca cacgcccggc gcgcgcgagc
     6961 cgcagttcac ttttctgcgt gcccacgtgc aggtcgccga cacggaagtc caataggtga
     7021 cttcgcccgt cggagacgta ggagcggcct tcgcgcaagc cctcgaccca cgcgcgataa
     7081 tcaagcgcgc cgtccagacg gacgtaagag cgacccatgc cgaggcggtc gccgtagatg
     7141 caggggaagt ccgtctcgcc cgcgatgcgc gtgcggaaac cgctgttgag cgtgtgatac
     7201 cagatgttga gttcccagac cgccggagta tcaaccgcag agatgaagtc tacagcgtcg
     7261 tgcgtcacgt ccacgatgta ctcgttcgcc ccgatcccgt caaaggaggg catctcgtag
     7321 ttcggcagtt tgtctgtcct gacttcaagc ccccagccgg agtgtgcgaa gccgacgatt
     7381 gcgccctgcg cccgcgccca tttgagaatc ggcaagcccc acgtcggcca atcgtcgatc
     7441 tccttcgcgc cgggatagtt ctgatccttc aagccgagca ggttgaggtg gccgacgtgg
     7501 ctcgacggga agccggagac ttccacgtcg tagcgcatca ggtgtgcggg cgtcgaaagc
     7561 ctgttcagtt tcgcctcgaa gaactgcttc tggaagtagt aacaaggccc ccacgtcagg
     7621 acgttgccga cgttgagtcc ctcgcccttg atgtggcgga tgatgtcttc ggggaagacg
     7681 ccttgcgtag gggcttcgta gtgagagcac ccggcggcgt ggatgtggtg atcgccggaa
     7741 taccatccgc ccgccgccgg attgatccag cgtttcaacc tgacggagaa ctcctgcggc
     7801 ttcgtccctc cggcgactct caaagtctcg cgctgcacga ggtattccgg accacgcgtg
     7861 acatcgatga tgtattcgcc cggcggaagc ctgagccgtt cgccgtcggc gcgatagacc
     7921 tgcgggtgga aggagaagtc cggggcgagg cgtttgtact tcgcagggta gatgcgttcc
     7981 tgacggtcgc ggatatggaa agaggctgtg gtcggcgtgc cgtcctcgtc gagcacgcgc
     8041 agcgtcacat ctttcgaagg aaggcagttg aagaggacgg acacgtcgtt gcgaaagcct
     8101 atgtcctgcg acccctgccc gacgttgaaa ctcacctgcg cctcgcgccg cccacggtcg
     8161 cggctataga gttggatgat gcgatactcg tgcagcagtc cggacaggcg aggttcgagc
     8221 ggttgtttat cgaacatcga aagatcgagc cagcggttta ccacctcgct cggtttgatc
     8281 ctctgcgtag gccgtgggtc gaggctgaac ccgcccggaa tcgtcgcgct ctgaccgcgc
     8341 gcgtagacgg gcagcgcgtt cggactcgcg gccttgagtt gcgccgtcac gcccgcctcg
     8401 ttacgcaccc tgacgaggaa cgaacgccaa cccttttcga cgagcgtggc ttcggcacgc
     8461 ccgcgcgtga cgcgcacgcg gctctccggg ctgatctcga cgaggaagag actgcgcgcg
     8521 tcgagcacct cgcggattcc cttgagcgcg agcgcaacat cctcctgctc cgaggcgcgt
     8581 tcgagcgccg cgcgttcggc ggggctgaac ggctcgccga gatactccaa cgcctcgacc
     8641 agacgcctca cctgtgcggc gagcggctgg agatcgaagc ccgcgtcatc cgccgcgcgt
     8701 ttcgcaccgg cagacgaagc gggcgcggcg acggcttgga cgacggcaaa cagcgagagc
     8761 gcgcaactca acgccagcaa gagcgcgcaa ctcaacgcgc gcagacgcac cagcgcgatt
     8821 tcacagaggg tactgccggg atgcttcatg gctcctctta acgttcgaga aggttgactt
     8881 ccctcacctg cgtctttccg ccccgttcgc ccgtgagcag gagcgtcttg atctcccagg
     8941 ggttgatctg cgccctgtgg ttgacgccgc gcgctgcgct ctcaagcgtg aacccggtgg
     9001 ctttgcccgc gcgctcctgc acgcggacga tggtgccctc gccgctctcc gcgcgtttca
     9061 acgaaagcag cgtaacgctg cccggcgaga ggcggaagaa tgaacgctcc cacggttcag
     9121 tcccttcgtg cgcggagtcc gtcacgtatt cggcgggcgt ctgcatctct tcggccagac
     9181 ggtcgagttc gagcgcggcg agatcgccct tgccgcgtac gagccagaag cgacgctcct
     9241 gcctgccctg gtcctgccac gcgccgtggt cgttgtgcgg cacctgatgc gggtcgtggc
     9301 gcgcgaaggg ggctgagcgg atgaggatgg tgcgcagcaa tccctgcttg cagtcgtagc
     9361 tgtaggtgga gttgttgacg aggccgagcg tgtgctgctc gccgccgatg cgtccctcga
     9421 ccgccaccca atcctgataa ggttcctcgt cgcccgtggg cgcgcgctcg gcgaccgcgc
     9481 cgggcactct ggcgaacgtg cgcggggcgt tgaacgccgt cggcacttcg agtttcaaga
     9541 tctgctcgcg ctcttgccag tctatgtgga agcgtagttc gatggcgtca atcgctttga
     9601 actgcgcgat gtcgagcacg atctcggagt tgcgccagcg tgcgcgctgg cgtatgacgc
     9661 gcgtgaccgc gccgtcctcg atctgctgcg tggagacgaa gacggggcga ccaatctccg
     9721 cgcgatacct cgtgatgccg tgcccccacg tgtcgctcgg gtcgccgatg acgacgaggc
     9781 cgatgggcgc ggccagcaac tctttgccgt ccgcagactt gagcgaggag atgccgaagc
     9841 cctcgtcgtt gacggagaaa aagttgttgt aaggcgcggt ttcgggcggc gtgccgtgca
     9901 ccacttcaaa gaccttgtag ccgcacgcgg gcaagtccac ccacgccgag atgcgtgggt
     9961 acacctgcgt catgctgtca gacgggcgga actggagcgg cactttctgc ccgtctttcg
    10021 tttcgagatg cgtgatgggg acgacgcccg cagggacggg cacccagggg ttcgagccgg
    10081 ggttccgctc cgtgtgatat tcaacgagac acttgcgcgg ccacggcagc gggttgaagg
    10141 cgaagaccgc gccctctctg accgtgcgcg tgtccacgcg ccgcgcgatg gcttcgagag
    10201 cctcgaccat gttggtctgc gccgtgtcgc aggccgcgcc caactggtcg cgcgcgtcgc
    10261 ggtaggccgt gtagagcgcg gttccggcca tcaggtcgtg aaactggttg aagagaactt
    10321 tgccccacgc ggcggcatac tcctccgcag ggtagcgttg gccgacgccg aggctggccg
    10381 cgtgtgagat cgtctcggcc tgtccgagcc aacgttcggc gcggcggttg atctgtttga
    10441 tctcgccgtg cgccgaatag acgccgcgcg agtggtattg aagttcgctt gagacgacgg
    10501 gcagggtcgc ggcggcgggc gacgcttcga ccgccttgaa gaactcgccg accgtgctcc
    10561 agcgcagttc cggcagcgac gtgtctttct gcatctccgt cacctgtcga atctgctcct
    10621 tcgtcacgga gccgccgtga ttgccgacgc cgagaaagta tgcggcgtgt tcgaagccgg
    10681 gcgcgaagtt attctcggcg gcgtctggaa tgcggttggc cgccatgctg tagccgcgcc
    10741 ggattctgag ggcgagcacg cgcgagccgt ccggcgactc ccaccagaac aggagcggca
    10801 gtttcatctc gttctcctgc gggcgcatga agacgtaata acgatagccc gcgcgccgca
    10861 ggatggacgg cagccccgcc gggtggccga acgagtcggg gttgaaaccg atctggatgt
    10921 caacgccgaa ggcgcgttga aaatagttct tgccgtagag acagtggcgc acgaggcttt
    10981 cggtcgaagg gatgttgcag tcgggttcga ccggccagcc gccgacgagt tcccaacgcc
    11041 cctcgcgcac gcgctctttg atctctgcga acatgcgcgg gtcggcacgt tcgacccacg
    11101 cgtaatgtgc agaggaagag tggctgtaac gaaagtcggg cgtctcgcgc atccggtcga
    11161 gcgcgctgcg catcgtggtg agcacggtgt tcgagccgtc gcgccaaggc cagagccacg
    11221 cggcgtcaat gtgcgaatgg ccgatgaggt gcagcacctt tcgccgcgcg aggctctgct
    11281 gcgcgccgcc cgccgctccc gtcctctgcg tcgcctgcgc gaacgcggga agatgcagcg
    11341 cggcgacgcc ccccagagcc gtcgcgcccg tcagcagaaa atgcctgcgc gagaaacctg
    11401 cctgcccgtc cgacgcgtcc gtcatctctt cgcttccccg ctctcgttta agattcaagt
    11461 tgtcccagag cctcgtcaat gtaaatgcac cagacgtgca gcagcatcag gtgaacttcc
    11521 tgcacgtgcg ccgtctggtc gctcggcaca gagaggacgt gatcggctgt gccgccgacg
    11581 agtcccgcgc tgccgacgac cgcgatggtc accgcgccgc gccgccgtgc ctcttcaagt
    11641 ccgcgcctca cgttctccga acgcccgctc gtcgtgaagc cgacggccac gtcgcccggt
    11701 gcgacgagag cgcgcacctg tcgttcgaag agcatgtcgt agccgaagtc gttggcgatg
    11761 caggtgacgg tgcccgcgtc gctcgccaga gcgatagccg ggaagggctg gcgcgtggcc
    11821 ttgaagcgac cgagcagttc gccgacgagg tggctcgcct gcgccgcgct cccgccgttg
    11881 ccaaaggcga tcactttgcg ccctgcgccg agcgcgctga ccagtgcgcg ggcggcctct
    11941 tccgccggtt ccgccagttg cgtgatgctc tcttctatca cgcgttgatg cgcagcgaag
    12001 tgctccgcga tgcgacccga ttttatctcc atctaacgct ttctcgcctg acggcaaccc
    12061 tagtctctta actagtcgct tgaggattgt tttaatgaac tgtgtttaag acccggtggg
    12121 aatgttctcg atgcggcaca atataccaga ccccggcgac aggccgacaa agaattttcc
    12181 cacaaggaca aaacgcccac ggctttaacg cgtcgcacgc ctgctccact tcttccagag
    12241 cgcggcgtct tccagcatct tgatgtagac gccgccaacg accgagcgcg cctgaaagcc
    12301 gcgctgcttg ccgtcgagcg tcccgtacca atccgtgagc ggcacgcggt cgggcgtctc
    12361 gttaacaaat ttgtagagcg gagcgacgaa ggaacgaaac tcttccggcg accctgagag
    12421 cgtcgccgtc cagatgatcc agtctaactt cgtgtagctt tcccgactgt cgagaggcag
    12481 gccgtagcgg ttctgcttcg tccggtagaa gtcgagttcg cggcgcgcga cctgcggcgg
    12541 aaagagattc aggccgagca gtctgtccca cacgagatta tatttctggc tccaggtgcc
    12601 gggcttgtcg aaggccagac ggtaatggtc gccgtcgtcc gccagcttga cccaacgcgc
    12661 ggccatctct tcggcgagac ggcggtaagt cctgccttcc gtcttcctcc cggtcaggtc
    12721 tgcgagcagg gcgtagctgc cgagagcgag aatcgctttc agagagaggt tggtgttgtg
    12781 cgcgagatgc cctgcgaagt cgtccgtgct caactggttc tccgggtcca tgcccttgtc
    12841 gcgcaggtac tccgcccagc gtgtgagcag cggccagtaa cggtctgcga agcgcgcgtg
    12901 gccgtcggcg cgtgcgagcg cagccgagag cagcagcatg ttgccgctct cttcaaccgg
    12961 catctggtct acttccgaag tttccctgcc gccgtaagtt tgaccgttgg caagcgggta
    13021 agtgccgagg tcgtgcggcg cgaaggggaa acgccagcgg ggcgaagcgg cgtagtcgaa
    13081 gacgggtttg agttgcgctt cgagcaggcg cgggttgaag agcaggaaga agggcgcgga
    13141 cgggtaggtt acgtcaacgg tggcgattga gccgttgctg tagttctctt tggagaagaa
    13201 gagcggcgtg ccgtcccagt ccgccgcgag cttgtgcgcg gcgatggtct ggcggaaagc
    13261 gagcgcggcg aggcgcgcgt attcctcgcc cccggcgcgc ctgaggtcgg ccatcagttc
    13321 ggcgtcgaag gcttcgctgc gccgcttcaa cgcttcgtaa tcgcgcgccg ccgcctgcaa
    13381 catcgcaccg gcctcgacgc cgtcgcgtcg ccagtaaggg cgcaggcggc ggcggaaata
    13441 ttcgacggag tacaggtcgt cgtaggccag catcaggtga cgcgagacgg gcgcggcggc
    13501 gacctcgccg agatcaaacg tcgtcgccag caccggcatc tcttcgcgtg ccatgcgcgg
    13561 catacgcatg tcgtccgaat cgggcagcgc gcccgtcgcg gcaaaactgt tgcgcgccgt
    13621 ctggttccac gcgatcacgt cggaggctgt gtgagcggcg gaggccggga cggatagatg
    13681 caggtaaccc caatcgatcc gcaggtcgtc gcccgcgcgc tccaacaccg gctgcgcctg
    13741 agaaccgaag gtgagcacac gcaggccgcc gaccttcggg cgcgaccaga cgaccttttc
    13801 ataagtcgtg ttgacggcga actccgccgt gcagtcgaag tagagcgaga cacggtgact
    13861 gcgcccgtca accgcccacg catcccacgt caggtacgtc acgggacgcg ccagcacttc
    13921 gagatcgtcc ggcagcagcg gactcataaa cgtgagacga agccgcacgc cttcggcctc
    13981 aaactcgtag agggtgcgcg tcgggagcac ttcgaggcgc acctgccgca tcggcgcggg
    14041 cagacggctg taggactgcc ccatgaagcg gtgaggcttc ccgtcaatcc gcgccatgcc
    14101 gaccatcccg tgcggcgcgc ccgtccagtg cttcgtgcgt tcgtcggcca gcgcgtcgct
    14161 tgtagaccag acgctgaagt aagggtcgtg cgtgacgagc gggacggcgg gcgggcgcag
    14221 tagcggcggc ggcacgagag cttgcgccga cgtttgaacg acgaagaggg cgacgaggca
    14281 acaggcgaca ggggcgcgaa cgcgcgttcg tgcaggcatg gcttgaaact ttcgggatgg
    14341 gaggacgtgt cgaaagagcc tgtggaacat agagaaagtg gagaacgtgg agaacgtgga
    14401 gaacgtggag aacgtggaga acatagttcg gctcatgtgg cagcgggggc gaaggcagcg
    14461 gggacgaagg ttggacgcgt tcatcgtgtg gcctgcccgc cctgcgtctt accggcttga
    14521 tcggcgagca cggccagcag cagcacgacg ccgagcgcga tccgctgcat gtacgggtca
    14581 accccggcga ggttcagacc gttgttgaga acgccgatga agaccgcgcc ccacaacgtc
    14641 ccgatgacgc tgccgcgtcc acccgtcaga gacgtgccgc cgacgacggt ggccgcgatc
    14701 acgtccaact cgtattgcag ccccgcgttc ggcacgcccg cgccgagccg cgacgcgagc
    14761 accagaccgg ccaagcccac cagcagcccg ttcaacacgt aagggagaaa ggtgaagagt
    14821 ttcgtgttga cgcccgcgtg gaaagcggcg cgcgggttgc cgccgagcgc gtagatgtaa
    14881 cgcccgaaga ccgtccgcgt taagagcacc cacccggcgg cgaagacgac gaacatgatg
    14941 atcaccggca cgggcagacc gagcacgcgc tcgcgcccga cgaaagcgaa ggccgccggg
    15001 aggttactga tggagcgacc gcccgtgatg gtgaagacca gcccgcgaca gatcgtcagc
    15061 atggcgagcg tcgccacaaa cggcggcacg cgaaactgcg cgacgacgaa gccgttaaag
    15121 attcccacgc ccacgcccac gccgagcgcg gcgaccgaac cgaggatgac cgaacccgtg
    15181 ctcccgcccg ccgccgcgat catcgccgcc accacccccg cgagcgcgac gaccgatccg
    15241 accgagaggt ctatgccgcc cgtcaggatc accatcgtca tccccacggc gaggatgccc
    15301 gtgatggcga tggagagcgc gatgttgatc aggttcggca cggagaagaa ctccggcttg
    15361 atcaaggcga acaccaccga ctcgatcagg atggcgaaga cgatcacgta ctccgtgacg
    15421 cgcccccact cagagacgcg agcgcgcagc ccgacgccct tctccgttgt cacttggctc
    15481 atttaatgtc cgctgtccgg ttggaagatg ctctggtgaa cgccggttcc ggcgcgcgtg
    15541 atgctctatt gtccgcctga cgctttcagc gattcggcgt cgacgatctt gacggggacg
    15601 gagatgctcg cgggcgactg cccgccgctg aagtgtgtgc gaatcacctc gatggtcttc
    15661 gcgccgaggt cgcgcggctg ttgcgccacg tcggccttga gttgactgcc gcgctggatg
    15721 gccttgacgg cttcgggcgt cgcatcgtag ccgacgatga cgagatcgct gcgcccgcgc
    15781 gcttcggctg cggagagcgc gccgagcgcg ctgtcgtcgt tgatgccgaa gatggccttg
    15841 atgtcggagt tgccctgcaa catgtcctcc gccgccttga gcgaacggtc gcgcacgccc
    15901 ccgccgttca gcacggcgac cactttgacg ttcgggtgtt tcgccgcgag ttcctccttg
    15961 aacccgttct cacgatccag ccccgtctgc acctcctgct gcccgatgat gccgacgctg
    16021 cccgcgccgc cgatggcgcg cgcgatgaag tctgcggcga gccgcccgcc cgcgagattg
    16081 tccgaggcga cgtgcgagac gacgcgcccg ctctgcgcgg cgatgtccgc cgtgaagacg
    16141 gggatgcccg cgttgttggc ctgctcgatg gcggggccga tgcctttcga gtctgcggga
    16201 cagacgatga tagcgtccat cttctgcacg acgaagtttt cgatctgcga ctgctgcttg
    16261 gcgaggtcga agtcaccgga agtaaccgtc agttgataac cctgcttatc ggcggcgtcc
    16321 ttcaagcccg cttcgaggtc gcgataaaac tcgtgctctc gcgtcagcag cgtaaccccg
    16381 atgcgcttag ttgaagaaga agaagaagaa gcggagccgc cctgatcggt gcgctggcaa
    16441 ccggccatga gcgagaaggc tgcgagcagc agtgatagac aggcgagacg tacaaacgat
    16501 ctcattatgt gttaggtaga cctttcaaga agggtgaaaa gagtgcatag aaaatcataa
    16561 agtcgtggag atgtcaaaac gaatcttcta ccgcgcacgc ctgcgcctcc acttctctgc
    16621 cttccggcgc gcggctcaac tacccctgac gacagttgcg actcgcaacg gagcgagtat
    16681 aatcccgcgc cgccacgcgt tgtcgccaga cgcttttttg ctcccggcgg aacagtcgcc
    16741 gcagagggag tttgcgcttt aggcagctcc cgtcaattaa aactaactcg attcggaaac
    16801 tcgccctgtc aggccatatg aagaaaagaa agatcgtgct cattcacacg tcgcccgccg
    16861 ccatcgctcc gctgatgcgg ttctacaacg acgccgcgcc ggagttggag atcactaacc
    16921 tgctggacga cgggctgctg cgcctgcttg cctctaagga gcacgcgact gtcgaagagc
    16981 gtctggcgga gatgctcgaa gtcggcgtcc gcacttacgg cgcggagttg gtcatggtca
    17041 cctgctcgtc agtcacgaag gggatggtcg agagactcgc gccgctcttc gatctgccgc
    17101 tcttgaagat agactactcg ttggcgcgcg aggccgtcag cgcgagacga cggaggatcg
    17161 gtctggcggc caccttcccg cccaccgtcg ttcccacttc tcaactcatc tcggaggcgg
    17221 cagaggaggc gggcgcggca gtcgagatca ttcaggaagt agtaccgggc gcatacgacg
    17281 cgcttctggc gggcgacgaa gaggcgcacg atgaactgct ctgcgccgga gtcgagcggc
    17341 tcgccgccgg aggcgtggac gtgatcgtgc tggcgcagat ttctatggcg cgcgtcctgc
    17401 cccggctcga aggaagggtg gcagtgcccg tgctgtcgag tctgcacacc agcctgagcg
    17461 cgatccgcgc cgcgcttaaa tagaatagat gacgagcgac tctccaacgc tttcacacga
    17521 cgcgcccgtg cacgacatcg aacgcctctt gttcgcggcg gtggccgacg acgacacggg
    17581 cgcgagcgat ctggcgggaa tgctggcggg acagggcgtg cgcacgctcc tggttctgga
    17641 cttgcccacg cccgcgcagt ttgccggatg gacgcgcggc tacgaggcgg tggtgctggc
    17701 cgaagggacg cgcaacctcg cgcccgcgct ggcctatgaa cggacgcgcg cggccatacg
    17761 gcttttgcag acgcgctgcc cgcgcctctt tcaactcaaa tactgtagca ccttcgattc
    17821 aaccgccgag gggaacatcg gcgcggcgat tgatgccgcc ctcgacgaac tcgcggagga
    17881 gttcaccgtc gccctccccg ccctgcccgt caacggtcgc accacttatc agggttatca
    17941 cttcgtccgt caacaactgc tgagcgactc ttccatgcgc aaccacccgc tcacgccgat
    18001 gaccaacccg aacctgatcg aactcctcgg ccaacagacg aagcgacgcg tgggactcgc
    18061 tccttatcag gtcgtgcgcg agggcgcggg cgtgctgaaa aagcacctgg acgaactgcg
    18121 cgcgggcggc ttccacgtcg cgctggtgga ttgtctcgac gacgaacaac tggaacagat
    18181 ctgccgcgcg gtggccgacc ttcgcctcat cacaggcagt tccgccttcg ggatgaagct
    18241 gcccgccatc tggcgcgagc gcggcttact cgaacggcag gccgcgcctc tcccgccgcc
    18301 tccgcgcacg gagagcgacg cgggctgcct gctcgtcgcc ggtagttgct cggcggccac
    18361 tttgcagcag aacgcctggg cggcggagca aggcatccgt gtacgacaga tcgctcctcc
    18421 acgcctgctg aacggggaac cggagcgggc agagatcatc gcggaggcac gccgcgagtt
    18481 ggctgccgga aggcactacc tgctgaccac ctccggcgca gcggcggaag tgcgagaggc
    18541 gcaggcgtgg ggagccgcgc gcggtctaac cgtccccgcg ctcggcgagt cgctggccta
    18601 cgctttggcg gaactcgtgc tggaagtgct cgaagggcag acgctcggcg gcttgatcct
    18661 ggcgggcggc gagacttccg gcgcggtgtg caggcgactc gaactcggcg cgatgcgcgt
    18721 gggacagaac atcgaaccgg gcgtgccgct ctgctattcg ctcggacgtt tccgcctgcc
    18781 tctcgttctc aagtcgggca atttcggaag tcccgatttt tacgggaagg ccacgcgcgc
    18841 cgtagcacgc agcggcgaat atctgacgat ctaacctgcg tcgtcaggcc gcgcgtcgca
    18901 cttgcccttg cgcgacgcgc tagcgtgcga ctcccgtgcc cgcgaaaaat ttgtagatgg
    18961 tcgtcgagtt gaaatgtccg cccttgcgca gcacggtcgt ggggaagtcg ggcttgttcg
    19021 gcgagtcggg aaaatgctgc gtctccagac agaagccctg ccgcgcgccg taagccttgc
    19081 cgcccttgcc gatcatcgtg ttctcaagat agttgccggt gtagagttgc atccccggct
    19141 ccgtcgtcca gacttccagc acgcgccccg tagagggttc gctcaccgtc gccgcacgcc
    19201 tgagcgcgcc tgcccgccct ctgatgacga aagtgtgatc gtagccgttg ccgaacttca
    19261 actgctcgtc gtcctgcgcg atgcgcgccc cgatggcctt cgcagcgcgg aagtcgaagg
    19321 gcgtgcccgc gacgcttcgc agttcacctg tcgggatgga tttcgcgtcc gccggaagga
    19381 agcggtcggc gtcgatggtg aggtggtgtt tgagtatgtc gccgttgccc tgtcccgcga
    19441 gattgaagta ggcgtggttc gtgaggttga cgaccgtgtc gcggtcggtg gtcgcagagt
    19501 agtccatacg caattcgttt ttcccggtca gcgtgtagac gactttgacg gagaggttgc
    19561 cgggatagcc ttcctcgccg tctttgctga ggtaggtcaa ctcaaccgcc gcgccgtcgc
    19621 gcgatgcgaa cggcctcgcc ttccagacga ctttatcgaa gcccttgagg ccgccgtgca
    19681 ggtggttctc gccgttgttg accgccagtt tgtactcctt gccgttcaac gcgaaacggc
    19741 ctctggcgat gcgattggcg taacggccaa tgaccggccc cacgtagaag gtggattggc
    19801 gatagccttc gagagtgtcg tagccgagca gcacgtctgc gaagcgtcct tcccggtcgg
    19861 gcactttgag cgagacgacg gttgcgccgt agttggtgat cttcgcttca accccctgac
    19921 ggttggtgag cgtgtagagt tcaacctgct gtccatccac tcgaccgaac gcctccgcgt
    19981 ggacaggttt gggtcttcgc tggaagccgc acagcagcac gacggcagac gccagcatca
    20041 atatagttat ccctttgcgc ttcatcttgg tttgcttagt ctcctcacga atgacttttt
    20101 tgttatgaat caggccggtg gccggaagtt cgcgcgggtc ttttgcaccg gggaaccggg
    20161 cgtggccgca catcataacc gagttcaacc gaactcgcca aagtggctcg cggcgacgcg
    20221 cgtgcaagcc tcccccgaag gctatctcgt gcacggcttt gcgacgaggc gttttggtat
    20281 gagagtatac gcggcaattt ggaggaaggc gggagagttg ctcctgctcc ccctgtaaag
    20341 ttgatctcgg tcgcggcgca ttggcggcat cgtcgccgtc gcaccacgcc gcccgcgtta
    20401 cagggggcgc gaaaaatttc cgtccggcgc acgcgctttc cgtcgctcag tgatattatc
    20461 ggcggcgcat gaaaatggca cgcagcgcat cacggcagcg atcagtgagc tgtcaggacg
    20521 cgcacccggt tataagttaa accgaaggac gcgcgccatc ccatcaaagt cataccgaat
    20581 ttaaacaatt taactttagg agacgcacat gaaacttagt acccgcacgg cacttgtcag
    20641 cttaacgctc ctgttaactc tctcggcctg ccagcagaac agcacgcaga ccggaggcgg
    20701 aggtcaagcg tcaggccagg gcggcaagaa attcgccttc gtgaccaaca acgcctccga
    20761 cttctggact atcgcgcgac gcggaaccga gaaggccgac gcggagttag cggatgtgac
    20821 cgtagagttt cgtatgccgc ctgacggcac ggctgcggac cagaagcgca tcgtggacga
    20881 cctgctggcg aaggggatcg acggcatcgc catctctccg gtcgacccgg ccaaccagac
    20941 gcaactgatc aacgacacag cccggcaggc tctcgtcttc acgcaggact cggacgcacc
    21001 gcagagtgat cgcgcggctt acgtcggcac ggacaacatc gccgccggtc gtcaggccgg
    21061 agagttgatt aaagaggcca ttcccgaagg cgggcgcatc atgctctttg tcggcaagtc
    21121 cgatgcgcgc aacgcgcagg agcgcacgca ggggatcagg gagtcgctgc aaggttccaa
    21181 cgtcgagatc atagacgtgc gcacggacga caccgaccgc gtgcgcgcaa aatccaacgt
    21241 ctccgacacg ctcgtcagat acccggaggt gaaagctctc gtcggcttgt ggagttataa
    21301 cggaccggca atcgtgaacg ccttgaagga gtccgggaag acgggtcagg tcaagatcgt
    21361 cgccttcgac gaagaggacg agacgctcgc aggggtgcgc gacggctcga tctacgcgac
    21421 ggtcgtgcag cagcctttcg agttcggcta tcaggccatc aagatgatgg cgcaggcggc
    21481 caaaggcgac cgcagcggga ttccggcgga caaaaaggtc atcgtcccca cgctcatcat
    21541 caagcgcgac ggcatcgaag agttccaacg caagatcaac gggcttcgcg gcagaagcta
    21601 acttaagttc tccgcgcgcc ctcaagtgta ggctgcgtct tttgtgtcga gcgctgtttg
    21661 atcaggcatt cgacaccgca ggcgcacagc ccacatgagc ggggcgcgcg gagagtgaca
    21721 cccggtggca aacttatacc gggcgcaacg ggtttgagac tatgactcag attctcaaaa
    21781 tgcagggcat cgaaaaacgc ttcgggggcg tgcatgcttt aaagggcgtt gacttcgaca
    21841 tctctgcggg cgaggccgtc gcgctcgtcg gcgagaacgg cgcgggcaaa tctacgctga
    21901 tgaaaattct cggtggcgtg caccagcccg atgccgggag catctcgatt gacggcgcgc
    21961 ccgcacaaat ccactccgtc gccgacgcca tccgccacgg cgtctccttc atccatcagg
    22021 aactgaacgt gctcgacaat ctggacgtgg cggccaacgt ctttctcggg cgcgagccgg
    22081 tgcgcggcgg ctttctcaac ctgatcgacc gcagacgcat gtacgcggag acgaaggtgt
    22141 atctggatcg tctcggcctg cacgtgccgc ccgacacgcc gctcagtaag ctctccatcg
    22201 cgcagcagca gttggtcgag atcgcaaagg cgctctcgtt gaacgcccgc ctgctcatca
    22261 tggacgagcc gacttcgagt ttgaccctga cggaaaccga ccgcctgctc gcactcgtca
    22321 aggagttgaa ggcgcagggc gtcgccgtca tctacatctc gcaccgtctg agcgaggtgg
    22381 aagagatttc ggatcgcgca gtggtcttgc gcgacggcgc gaacgccggg agccttgcgc
    22441 gcaaagagat cacgcacgac cagatggtca agatgatggt cgggcgcaac ctcgacaatt
    22501 tctaccgcca gccgacgggc gcggtcagtt ccggctactt gcaggtcgag cgtctgcgca
    22561 cgagccgtta cccgcaacag acgatctctt tctccgtcgg caaaggcgag attctcggtc
    22621 tggccgggct ggtcggttcg gggcgcacag aggtggcgga ggcgatcttc ggcgttgacg
    22681 gcgcgagcga cgcgcagatc acgctcgaca gccgcccctt gcgcgtccgt tcgccgcgcg
    22741 acgcgatccg gcacggcatc tacctgatcc ccgaagaccg acgccacgcc ggactcatca
    22801 ccgacatgat gattcgtgag aacgtcaccc tgcccgctct cgaacgctac gcctccgccg
    22861 ggcttgtctc cttcgaacgc gagcgcacgg cgg
//
LOCUS       DASWNS010000272         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_479635, whole genome shotgun sequence.
ACCESSION   DASWNS010000272 DASWNS010000000
VERSION     DASWNS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479635"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1068
                     /locus_tag="VGB76_09655"
     CDS             238..1068
                     /locus_tag="VGB76_09655"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0099207.1"
                     /translation="MSENKTDKELAFLHDLYIAPDWGERFAGLMDEHVQLPKTGHALY
                     VAAGTGGHALALKERAGEDLTLIGVDESEARLTLARAKAAATKLNEVTEFRAAQLEAL
                     AFEDEQFDLVVGDASLVAVERLPEVLAEMVRVAAPGATVALTLPTASSFGEFYSIYWE
                     ALMNAEMLEESRIVEQLIQERPTVSDIETLAAREGLDLVRSWTNIEEFDYASGEEFLN
                     APLVKDHLLKNWLEPLDEDEAALARVLSEVRRIIDAERDESEFALTIKATLVVGQKTE
                     "
     gene            complement(1162..1434)
                     /gene="tatA"
                     /locus_tag="VGB76_09660"
     CDS             complement(1162..1434)
                     /gene="tatA"
                     /locus_tag="VGB76_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY0099208.1"
                     /translation="MHEITLAIGFGTQELLIILLIVFVLFGASRLPQLAKAIGQSKRA
                     FKDGLDEADEEARIEAARRSERLRAAESNLREDELSTDKYTANKPR"
     gene            complement(1496..>1730)
                     /locus_tag="VGB76_09665"
     CDS             complement(1496..>1730)
                     /locus_tag="VGB76_09665"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0099209.1"
                     /translation="PRSRGGESTWDNLVACCKRCNHRKGNRTPEEAKMHLIRHPRGFS
                     LHVSRQIMRYLGRADQTWRKYLFYESDNGESGE"
BASE COUNT          363 a          489 c          528 g          350 t
ORIGIN      
        1 cccgcgcgcc agagacgaaa tggcctgcct gcccgaacaa ctgcgcggcc acgaatatta
       61 cagagacgac gaggaacacg gaacgatgaa tgatgaatga aaagcaggag ccggaattca
      121 gcacaccgga ctcagaagca atcgaacccc ggcgcatgct cgtcttctga ctcccgtatt
      181 ctgaactctg tattcccttt catcattcat cgttcatact tcatcatcta attttttatg
      241 agcgagaaca agacagacaa agaattggct ttcctgcacg acctctacat cgcgcccgac
      301 tggggcgagc ggttcgccgg attgatggat gaacatgtgc agttgccgaa gacggggcac
      361 gcgctctacg tcgcggcggg cacgggcggg catgcgcttg cgctcaagga acgcgcggga
      421 gaagacctga ctttgatcgg agttgacgag agcgaggcgc gactcacgct cgcgcgggcg
      481 aaagcggcgg cgacgaaatt gaatgaagtg acggagtttc gcgcggcgca actcgaagcg
      541 ttggccttcg aggatgagca gttcgatctc gtagttggcg atgcgtcgct cgtagcggtg
      601 gaacgcctgc cggaagtgct ggccgagatg gtgcgcgtcg ccgcgccggg cgcgaccgtg
      661 gccttgacgc tgccgaccgc ttcgagcttc ggagagttct attcgatcta ctgggaagcc
      721 ctgatgaacg cggagatgct cgaagagtcg cgcatcgtgg aacaactcat tcaggaacgc
      781 ccgaccgtca gcgacatcga aacgctggcc gcccgcgagg gtctggacct cgttcgctcc
      841 tggacgaaca tcgaagagtt cgactacgcc tcgggcgaag aatttctcaa tgccccgctc
      901 gtcaaagatc acctcttgaa gaactggctc gaaccgctcg acgaagatga agccgcgctc
      961 gcacgcgtcc tctcggaagt caggcgcatc attgacgcgg agcgcgacga gtcggagttt
     1021 gcgctgacga tcaaggccac actcgtcgtc gggcaaaaga ccgagtagac gcctttccct
     1081 gtcgaacgga agagccgccg gagtcgtttt gagcgaccac ggcggctcta gtcttaatat
     1141 tcaaacgttt gggagaattg tttagcgcgg cttgttggcg gtgtacttgt cggtcgagag
     1201 ttcgtcttct ctgaggttcg actcggcggc gcggaggcgt tcgctgcggc gtgccgcttc
     1261 gatacgcgct tcctcgtcgg cttcgtcgag gccatctttg aaggctcgct tgctctgtcc
     1321 gatagccttg gcgagttggg gcaggcggct cgcgccgaaa aggacaaaga cgatcaggag
     1381 gatgatcaga agttcctgcg tcccgaatcc gatagctagt gtgatctcat gcatcgctta
     1441 aattccttta cagataaacg tctagtgtca gccaaaaaag atctcgctcg cgccgctatt
     1501 cgcccgactc cccgttgtcg gattcataaa aaagatactt gcgccacgtc tggtcggcgc
     1561 gtccgagata acgcatgatc tggcggctca cgtgcaacga gaagccgcgc gggtggcgaa
     1621 tcaagtgcat cttcgcttcc tcgggggtgc ggttgccctt gcggtgattg cagcgtttgc
     1681 agcaggccac cagattatcc cacgtcgatt cgccgccgcg cgagcgcgga
//
LOCUS       DASWNS010000273        16289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_426243, whole genome shotgun sequence.
ACCESSION   DASWNS010000273 DASWNS010000000
VERSION     DASWNS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426243"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VGB76_09670"
     CDS             <1..668
                     /locus_tag="VGB76_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099210.1"
                     /translation="HPKLTPNLVKMLLMYTAQPLSGFNMFEQGAGQLNIEGAVRLAKL
                     VRTDLSPLTSAGSPLLTTANAPVPETTIAGHKFKWSQGLIMGRTFATGSNLALKYQKI
                     YSTGVIFGDSLIFGDGVIFGDQTLLSSGVIFGDNVLLSDGGTLASGTPLLNVDALIAD
                     GVIFGDGVIFGDGVIFGDGVIFGDSALRGDSITQAQSARTSGDLTSAMELEVDTGVDY
                     IGY"
     gene            737..1681
                     /locus_tag="VGB76_09675"
     CDS             737..1681
                     /locus_tag="VGB76_09675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0099211.1"
                     /translation="MPHSIQAREATPAAKKQTPISSSAPVALRLPEIHTLADAHYGEV
                     LTFLDAVRSVDTIFMSGLIRDNGVVSDFNRGAFYGCRDAQGQLEGVALIGHATLVEAR
                     SEGALAAFAHLAKEAQAHVIVGDQEKVERFWNHYEKGGQKPLRICRELFLEQRWPVET
                     LTEAAALRLATPEDLEAVMAINARMACDESGVNPLETDAEGFRARLARRIELGRVWVW
                     TDSTDGRLLFKVDIMAEVPGCIYLEGVYVHPEDRRRGYGRRALSQLGRHLLARTETLC
                     LLVNEQNKDAQIFFFKAGYKLRACFDTIFLQPRAGESH"
     gene            1837..3855
                     /locus_tag="VGB76_09680"
     CDS             1837..3855
                     /locus_tag="VGB76_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099212.1"
                     /translation="MNRQRFAKPFMWSVIAVGALVCLYSIYHLPLGSLDLLFLLLAFA
                     TVITSHITVPMPGVNGRITVSETFIFLTMLLYGGAPAILLAAIDGFCSSRLISRKPIT
                     LGYNAAQLAISTGITVLIVRFFFGNVVNLTHGDFSVNFLIAVCIMGFVQHICNSLLAS
                     IGQALKHDQSIWRVWSRYCLWTSMTYFAGASAAVITAKLVGMFGPYAIIATTPIIASI
                     YLSYRTYLKNIQVMAAAAKAEAAAAARAEAAKEQAAQAELHVEELSHYIAEQERIREQ
                     YTQIEKLSALGELASGVAHDFNNTLAGILGRAQLLLNTNDPAKIESGLQIIIKTAKDG
                     AKTIKRIQDFARQRRDHAFSPVAVDQLLLDVSEITRPRWKDHAEAASVHINLELKINS
                     KALVLGDESELREVLVNMVFNAVDAMPAGGTLTLATREVDGAVEISVADTGTGMSDDV
                     RSRIFDPFFTTKGKAGMGLGLAVSYGIIRRHEGTVEVESETGRGTSFRIALPSAKNAA
                     KPQPNAVQDTPQTTLAREAFRTKFLVVDDEESVRELLRDILEKEGHEVTIAANGRDAL
                     ALFAEAHYAAVLMDVGLPGMSGWELARAIRERDERIPLAIITGWGEAVGSSAQRAAGV
                     DWVVAKPFTVEQITHIAQEVARRRGGAAAKTAMATHAQTEHAATIVAA"
     gene            complement(3960..4166)
                     /locus_tag="VGB76_09685"
     CDS             complement(3960..4166)
                     /locus_tag="VGB76_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099213.1"
                     /translation="MFNGVDIIFFIPPLLLLIVATVVGLWQYSKHGAKRALLASFIVL
                     ALGGLLIFALLVVWLYIYYSSGGH"
     gene            complement(4215..5660)
                     /locus_tag="VGB76_09690"
     CDS             complement(4215..5660)
                     /locus_tag="VGB76_09690"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0099214.1"
                     /translation="MKALVIDDEHVIRDNVAEVLRSDGWHVSEAESAEHAFEMLSRET
                     WALVFCDVKLGGDGGHDGFAVLRRFTEEQPQAQIVLMTGHGSAVGALDAVASGAYDYL
                     MKPFDNDEVLAISQSVRGGIEKRARQQTTGDLLPPVYTSDIDLVGQSAAFVEMMKLVG
                     RVAGTNLPVLITGESGTGKEVVARAIHRRSQRAEQAFVAVNCGAIPADLIESELFGHV
                     RGSFTGATVDRRGLWQEAHLGTIFLDEITETTPAFQVKLLRALQEGEIRRVGSNHTLQ
                     VDVRVIAATNRDVEREMREGRFRQDLLYRLNAVTLHLPPLRERSEDILPLARRFAERA
                     KRAGERPVSFSREAVRILESYNWPGNIRELENAIVRATALCDGIVHPEDLPERISKAI
                     RDDAAAAAPSGGVAAAAVAAAAAQTGANESASELVSLSELECRHIKRVLDHTHGNKQA
                     AARLLGIDRSTLQRMIKRHNLEEGKTDSDCV"
     gene            complement(5756..6187)
                     /gene="dut"
                     /locus_tag="VGB76_09695"
     CDS             complement(5756..6187)
                     /gene="dut"
                     /locus_tag="VGB76_09695"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY0099215.1"
                     /translation="MTDELKFLRLHPAAKLPTRGSRQAAGLDLYAIETLTLEPGARTA
                     ARTGLAVAIPEGFYGRVAPRSGLAVNYGLDVLAGVIDSDYRGEILCALVNHGREACTI
                     EAGQRVAQLIVEAIIAPEPAWADALDETARGAGGFGSTGSV"
     gene            complement(6309..8657)
                     /locus_tag="VGB76_09700"
     CDS             complement(6309..8657)
                     /locus_tag="VGB76_09700"
                     /inference="COORDINATES: protein motif:HMM:NF005829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEY0099216.1"
                     /translation="MRLIITEKPSMGRDVAAALGATRRGEGYLEGKDDIITWCIGHLV
                     ELDDPETYDPKLKSWRMETLPIIPEEFKYHASERTKDQFNVIKNLLKRADVTSIVNAA
                     DAGREGELIFDLVYTLARSRKPVERLWISSLTRDEIVAGFNRLKPAADYRGLRDSARS
                     RQQADWLVGLNATRAQTIKARGAGYDGVYSLGRVQTPTLALIVARDAEITNFVPTNYY
                     EVVADFQSAAGAYRGTWFNKQGSRFDHKEAAEAVVFKVQGKPGTVEKVEKKASKERAP
                     LLYDLTALQRAANARYSFSAARTLELAQTLYEKKFITYPRTSSRHLSTSVNQELKAHV
                     EAASVGPYVKFIETILAKGTPKLTSRHVDDKKVTDHHAVIPTKQRVEPNALQPDEKRI
                     YDLIARRFLAAFYPDAELERTTITTAVDGEQFITRGTVVLKAGWREVDPPGREERKKT
                     DGEEEAEDAELPQVRAKESVETTKVEAVAKQTKAPPRYSESSLLGAMETAGKKVEDEE
                     LRLAMKDSGLGTPATRAQIIETLLKREYITRDKKSLTATPKGIALIKLLPSQLLKSAE
                     LTGSWEQKLARMARGEYARDAFMGEVKEMVRELVGQIAASEMERATGDQVAKRADVER
                     PADSPDCPKCKLEERAGFLVERVSANGKFLVCSAGRDACGFISDAPKNAKQRKILLAT
                     KCAVCGGAMRLRAPREKGKRASLSCARYPECRGVRWFDEEGALEEAKALPESGQPCQA
                     CGAPTVKRGPFRNDSYFWGCTRWKSDKSGCNAPPVWINEARA"
     gene            complement(8654..8992)
                     /locus_tag="VGB76_09705"
     CDS             complement(8654..8992)
                     /locus_tag="VGB76_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099217.1"
                     /translation="MNSWLMKLKSLMFTRRIRDRGRREQVGAFIITARKNLHPDGIGC
                     AMCGKLLSRYDASSDTHAPPCEQLLAEGKVPIPNFGWFCSHGCADAYEGRYGVHFQRT
                     AEGKVDYYSE"
     gene            complement(9013..9867)
                     /locus_tag="VGB76_09710"
     CDS             complement(9013..9867)
                     /locus_tag="VGB76_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY0099218.1"
                     /translation="MRIAQTIFLLLLFAGASALLPVRAQTTNAARPLADTVSTREMDR
                     INWMEFREVVPSRVQTVLLPTGTLEPHGVANNGADNTAPLAIARRIAQRTNAMIAPVL
                     PYGMTGSMEAYPGAFQITEAAYRPFVKQILEGLAKNGFRNIIIINGHGGGQTAVLNSV
                     AAEVAAERRVRTLVVNWWSFASDVTKQVFGEDGGHAGWNETAFVQAIDPTLVHPERYK
                     DDLATPYPAAGTWSATPFPSSIGLYQPGQGYPKFDQKKADEYFVKVTDKIALLVNDVV
                     RKWDMAGL"
     gene            10180..11856
                     /locus_tag="VGB76_09715"
     CDS             10180..11856
                     /locus_tag="VGB76_09715"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0099219.1"
                     /translation="MSHSKEEAQQTSSLNRREFLGTTLTASIAAATTLAHPAPARAEP
                     LAPDASPLTNAPRAFELEETTIAALQEAMREGKLTAREIAGRYIERIEKFDRKGARLN
                     SIIELNPDALALAAALDRERKTTGARSPLHGIPILLKDNIDTADRMRTTAGSLALADT
                     FAPRDAFIVERLRAAGAVLLGKTNLSEWANFRSTHSSSGWSARGGQTRNPYAIDRNPC
                     GSSSGSGVAVAANFAAAAIGTETDGSIVCPASACSLVGLKPTLGLVSRSGIIPIAHSQ
                     DTAGPMTRTVADAAALLNTLAGIDPRDRATQTSRGKSHADYTRFLDPAGLRGARIGVA
                     RKFFGFNDKVDKLMADAIDALKRAGATIVDPTDLPSHGKYDDSEFEVLLYEFKADLNK
                     YLAERGGGAPRSLKELIDFNERNREREMPYFGQEIFLKAQAKGALTSKAYLAALAKNH
                     RLSRAGGIDAALTKHRLDALIAPTGGPPWTTDLINGDHFSGGSSTPAAVAGYPNISVP
                     AGYVFGLPVGISFFGTAYSEPVLIKLAYAFEQATRHRRPPQFTDSVDLRD"
     gene            12153..13790
                     /locus_tag="VGB76_09720"
     CDS             12153..13790
                     /locus_tag="VGB76_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEY0099220.1"
                     /translation="MTAWLPDLVYTGGGRFESGLALVSDRAGRITHLTRDVESTGAAR
                     IVRLQGRALLPGLVNAHSHAFQRVIRGRTERRSAAHDSFWTWREMMYAAATRLSPDDL
                     YDASRMAFLEMAASGISAVGEFHYLHHTPEGTRYADENELAKQVVRAARDVGLRIALL
                     RVAYARAGWNTPPNTRQARFIEHDAQTFLRQTETLKKDIARQMSNAVEASNAVEASND
                     VDAREESEAVGLDASASSTWESASSTRESASEAWVGVAPHSVRAVPLDYLREVNAFAV
                     AHDLPVHMHIAEQPAEVAACLAEHGRTPVALLADEGILGARFTAVHAVHITAEEARAL
                     AAERAHVCACPTTERNLGDGIIPADLLLGAGVRVSFGTDSHTQIDLLEDARELEYHLR
                     LQKLERAVLAPVTTGAHTTETNAARADTHEADMETDSLAALAARLFECATTNGASSIG
                     APGGSRNGSVAPVEGSNGGAFEIGGVADFFTVALDDLSIAGAGTEDLLPAIVFSLART
                     AVRDTVVGGRRILEDGQHRAQDEIIERFTRVQRKLWR"
     gene            13838..13981
                     /locus_tag="VGB76_09725"
     CDS             13838..13981
                     /locus_tag="VGB76_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099221.1"
                     /translation="MTNEEMQRIMEFTMQRQEAFTEQQEKLSASPDRLEQERVAETVR
                     KGL"
     gene            13978..15108
                     /gene="argE"
                     /locus_tag="VGB76_09730"
     CDS             13978..15108
                     /gene="argE"
                     /locus_tag="VGB76_09730"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEY0099222.1"
                     /translation="MSVAETLAELVAIDSVSSRSNLEIIKFLAGRAEAAGLCARMFPY
                     TDAAGVKKFNLVCVPPSVNAERMEVELALVGHTDTVPYDPAWTDALTLSVREGKFYGR
                     GSCDTKGFIAAALTAIEQVDLARLSKPLAFVFTADEEVGCIGAKHLAESRALAARYAI
                     VGEPTSLQPMRAGKGYCLAEVRVGGREGHSAYPALGASAIFRAARLIARIEGVAEKLQ
                     AEPHASFDPPYTTLNVGVIRGGTAKNIIAGECRFTLEWRPVPGQAATHTVELIQNEIE
                     SLRGVEPDFDCEVEILRLDEASETRPDSLLVNELETLSGQAAGTIAFGTEAPQLTEMG
                     AEAVVFGPGSIRNAHRTGEFVPADELAACVEILARAIERFCR"
     gene            15222..>16289
                     /locus_tag="VGB76_09735"
     CDS             15222..>16289
                     /locus_tag="VGB76_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099223.1"
                     /translation="MNQRIFTSASFRTMLGASLLIVLSVIHVAAQAQAQQQKPLTSQE
                     LVRLVYQLPSQPQKRDEIIEEIRRRGIGFELTTGIRSIVATKSGNDALLRRTLEEAAR
                     RRLNPAGAAARPSEREAEDALERTRKATREAAGAMPDFVVRQLVRRSLARGETRNFSQ
                     RDQLTVAVSYRESAGGEQYKLLAVNGLPVTEESRERGSYEQAGGTSSTGEFVSTLNEL
                     FADHTKTEFHAVDTDTINGRRAIIYDYAVKIDNSKQTLKVTESRGMEPLVARVGYRGR
                     VWIDREAFRVLRIEVISTDIPYDFPITAAVNTIDYDWVEISGRKYLLPIRSEVELTTR
                     YQQQTYQTRNEIRFRNYQRFGS"
BASE COUNT         3076 a         5276 c         4916 g         3021 t
ORIGIN      
        1 ctcatccgaa attaacgccc aacctcgtca agatgctcct gatgtacacg gcgcagccgc
       61 tctccggctt caacatgttc gagcagggcg caggccaact caacattgaa ggcgccgtca
      121 ggctcgccaa actcgtgcgc accgacttgt cgccgctcac ttccgccggc tctccgctcc
      181 tgacgacagc caacgcgcct gtgccggaga cgaccatcgc gggtcacaag ttcaaatggt
      241 cgcaaggctt gatcatgggt cgcacgtttg ccaccggctc aaacctcgcg ctcaagtatc
      301 agaagatcta cagcacgggc gtcatcttcg gcgacagcct gattttcggc gacggagtta
      361 tcttcggcga ccagacgttg ctctcaagcg gcgtcatctt cggcgacaac gtgctcttga
      421 gcgacggcgg aactctggcg agcggcacgc cgctgctcaa cgttgatgcg ctcatcgcag
      481 acggcgtgat cttcggcgac ggggtcatct ttggcgacgg cgtgattttc ggcgacggag
      541 ttatcttcgg agatagtgcc ttgcgcggcg attccatcac tcaggcgcaa tcggcgagaa
      601 caagcggtga tttaacctcc gcgatggagt tggaggtaga tacgggcgtt gattacatcg
      661 ggtactagga aaaaattgtc gctccacgca cttttgttcg agccgtagaa gtctgtagga
      721 gtataaggag acgctcatgc cccattcaat tcaagccaga gaggccacgc ccgccgcgaa
      781 gaagcagacc cccatctctt cttctgcgcc cgtcgcgctc aggctacctg agattcacac
      841 actcgccgac gcccactacg gggaagttct cacctttctc gacgcggtgc gttcggttga
      901 caccattttt atgagcggcc tcatccgcga caacggcgtc gtcagcgact tcaatcgcgg
      961 cgcgttctac ggttgccgcg atgcgcaggg acaactcgaa ggcgtcgcgc tcatcggcca
     1021 cgccacgctc gtcgaagcgc gtagcgaagg cgcgctcgcg gccttcgcgc atctcgctaa
     1081 agaagcgcag gcgcacgtca tcgtcggcga tcaagagaag gtcgagcgtt tctggaacca
     1141 ctacgaaaag ggcggccaaa agccgctccg catctgccgc gaactcttcc tggagcagcg
     1201 ttggccggtg gagactttga cggaggcggc ggctttgcgc ctcgccacgc cggaggacct
     1261 cgaagcagtg atggcgatca acgcgcggat ggcttgcgac gagagcggcg tcaatccttt
     1321 agagacggat gcggaggggt ttcgcgcgcg gctcgcgcgc cgcatcgagt tggggcgcgt
     1381 ctgggtctgg actgacagca cagacgggcg gctgctcttc aaagtcgaca tcatggcgga
     1441 agtgcccggc tgcatctatc tggaaggcgt ctacgtccat cccgaagacc gccgcagagg
     1501 ttacgggcgg cgcgccctct cgcaactcgg caggcacctg ctcgcgcgca cggaaacgct
     1561 ctgcctcctc gtcaacgaac agaacaaaga cgcgcagatc ttcttcttca aagcgggcta
     1621 caaactgcgc gcctgcttcg acaccatctt cctccagccc agagcgggcg agagccacta
     1681 agacttgttt gaaatccttt gccctgcctc tttgcgcctg aacgcaggtt tgaggcttgc
     1741 tccggtatga atctcaggtt cgggcgcaaa ggaaagagac gaaagcaagc agagcgagag
     1801 ccgtttaccc taacaaactc cgagaggatt cccctgatga acagacagcg tttcgctaag
     1861 cctttcatgt ggtcggtcat cgccgtgggc gcgctggtgt gcctctattc gatctaccat
     1921 ctgccgctcg gctcgctcga tctgctgttt ctactgctcg cgttcgccac cgtcatcacc
     1981 tcgcacatca ctgtgccgat gcccggcgtc aacgggcgca tcaccgtctc cgagacgttc
     2041 atcttcctca cgatgctgct ctacggcggc gcaccggcca tcctgctggc cgcgattgac
     2101 ggcttctgct cgtcgcgtct gatcagccgc aagccgatca cgctcggcta caacgccgcg
     2161 caactcgcca tctccaccgg catcaccgtc ctcatcgtgc gtttcttctt cggcaacgtc
     2221 gtcaatctca cgcacggcga tttctccgtc aacttcctca tcgccgtctg catcatgggc
     2281 ttcgtccagc acatctgcaa ttcgctgctc gcttcgattg ggcaggcttt gaaacacgat
     2341 caatcgatct ggcgcgtctg gagcaggtat tgcctgtgga cttcgatgac ttacttcgcg
     2401 ggcgcgtcag ccgccgtcat cacggcgaaa ctcgtcggca tgttcggccc ctacgcgatc
     2461 atcgccacga cgcccatcat cgccagcatc tacctgagct atcgcaccta tctcaagaac
     2521 attcaggtga tggcggcggc ggccaaggcg gaggcggcag cggcggcgcg cgccgaagct
     2581 gccaaagagc aggccgcgca ggccgaactc cacgtcgaag aactctcgca ctacatcgcc
     2641 gaacaggagc gcatccgcga gcagtacacg cagatcgaaa agctctccgc gctcggcgaa
     2701 ctcgcttcgg gcgttgcgca cgatttcaac aacacgctcg ccggaatact cggtcgggcg
     2761 cagttgctgc tgaacacgaa cgacccggcg aagatcgaga gcggcctaca gatcatcatc
     2821 aagacggcga aggacggcgc gaagaccatc aagcgcattc aggacttcgc acgccagcgt
     2881 cgcgatcatg ccttctcgcc cgtcgccgtg gatcaactgc tgctcgacgt gagcgagatc
     2941 acgcgcccgc gctggaaaga ccacgcggag gccgcgagcg tccacatcaa tctcgaactc
     3001 aagatcaact cgaaagctct ggtgctgggc gacgagtcgg agttgcgcga ggtgctggtc
     3061 aacatggtct tcaacgcggt ggatgcgatg cccgcgggcg ggacgctgac gcttgccacg
     3121 cgcgaggtgg acggggcggt ggagatttcc gtcgccgaca cgggcacggg gatgagcgac
     3181 gacgtgcgct cgcgcatctt cgatcctttc ttcacgacga agggcaaggc gggcatggga
     3241 ctcggactcg ccgtgagcta cggcatcatc cgtcgccatg aaggcacggt cgaagtcgag
     3301 tcggagacgg ggcgcggcac gagttttcgc atcgcgctgc cgtccgcgaa gaacgccgcg
     3361 aagccgcagc cgaacgccgt gcaggacacc ccgcaaacga ccctcgcgag agaagcgttt
     3421 cgcacgaagt ttctcgtcgt tgacgatgaa gaatcggtgc gcgaactcct gcgcgacatt
     3481 ttagagaagg aagggcacga ggtgacgatt gccgcgaacg ggcgggatgc gctcgcgctc
     3541 tttgccgagg cgcactacgc cgccgtgttg atggacgtgg ggcttccggg catgagcggt
     3601 tgggaactgg cgcgcgccat acgcgaacgc gacgagcgca tcccgctcgc catcatcacg
     3661 ggctggggcg aagccgtcgg ctcaagcgca cagcgcgcgg cgggtgttga ctgggtggtg
     3721 gcaaagcctt tcaccgtcga acagatcacg cacatcgcac aggaagtcgc acgcaggcgc
     3781 gggggcgcgg cggcaaagac ggcgatggcg acgcacgctc agacggagca cgccgccacc
     3841 atcgtcgccg cgtgaactgc gcggattaac tggccgaaaa aaaactctct cgtgcggtaa
     3901 gtcggagctg agcgacttgc cgcacgagag agtttttgcg ttgtctgatg ccgcgccggt
     3961 taatggccgc cgctcgaata gtaaatatat agccagacta ccagcaacgc gaagatgagg
     4021 aggccgccga gcgcgagaac gatgaagctg gcaagaagcg cgcgcttcgc cccgtgtttt
     4081 gagtattgcc acagcccgac caccgttgcc acgatgagca ggagcaatgg cgggatgaag
     4141 aagatgatgt ccacaccatt aaacatgatc ctcgtatatc tttccggtcg aataggtgaa
     4201 gcagactgaa cttcttacac gcagtcggag tcggttttac cttcttccag attgtgccgt
     4261 ttgatcatgc gttggagcgt gctgcggtct atgccgagca gacgcgcggc ggcctgcttg
     4321 ttgccatgcg tgtgatcgag cacgcgcttg atatgacgac attcgagttc ggagagcgaa
     4381 accagttccg acgcgctctc attcgcgccc gtttgcgctg ctgccgctgc tactgctgcg
     4441 gcggcgaccc cgccggaggg cgcggctgcg gcggcgtcat cgcggatggc tttgctgatg
     4501 cgctccggca gatcttccgg gtggacgatt ccgtcgcaga gcgcggtggc gcgcacgatg
     4561 gcgttctcca gttcgcgtat gttccccggc cagttgtaac tttccagaat ccgcaccgct
     4621 tcacgcgaaa aactgacggg acgctcgccc gcacgcttcg cacgctccgc gaaacggcgc
     4681 gcgagcggca ggatgtcttc gctgcgctcc ctgagcggcg gcaggtgcag cgttacggcg
     4741 ttcaggcgat acaacaagtc ctgccggaaa cggccttcgc gcatctccct ctccacgtcg
     4801 cggttggtgg cggcgatcac tctcacgtca acctgcaaag tgtggtttga accgacgcgg
     4861 cgaatctcgc cctcctgcaa agcgcgcagc agtttcacct gaaaggcggg cgtcgtctcc
     4921 gtgatctcgt cgagaaagat cgtgccgagg tgcgcctcct gccagagtcc gcgacggtct
     4981 acggtcgcgc ccgtgaacga gccgcgcacg tggccgaaca gttccgactc gatgaggtct
     5041 gccgggatcg cgccgcaatt gacggcgacg aacgcctgct cggcgcgctg gctgcgacga
     5101 tgaatcgcgc gcgcgacaac ctctttgccc gttcccgatt cgcccgtaat caacacgggc
     5161 aaattcgtcc ccgccacgcg cccgacgagt ttcatcatct cgacgaaggc cgcgctctgc
     5221 cctacgagat caatgtccga agtatagacg ggcggcagca ggtcgccggt cgtctgctgg
     5281 cgcgcgcgct tctcgatgcc gcccctcacc gattgcgaga tggcgagcac ctcgtcgtta
     5341 tcaaacggct tcatcaaata gtcgtatgcg ccggaggcga cggcatcgag cgcgccgacg
     5401 gctgagccgt gtccggtcat caggacgatc tgcgcctgcg gttgctcttc ggtgaaacgg
     5461 cgcagcaccg cgaagccgtc gtggccgccg tcgccgccga gtttcacgtc gcagaagacg
     5521 agcgcccacg tctcgcggct gagcatctcg aaagcgtgct ccgccgattc ggcttcgctc
     5581 acatgccaac cgtcgctgcg caacacctcg gccacgttgt cgcggatcac atgttcgtcg
     5641 tcaatgacga gagctttcat atcttcattt aactgagcgg tcatgattcc agaattcaag
     5701 actctccgcg agcgtcgcgc agaaacaaaa gacgcaatgc cgccattatt tcaccctaga
     5761 cgcttcccgt gctgccgaag ccgcccgcgc cgcgcgccgt ctcgtcgagc gcgtcagccc
     5821 acgcaggctc cggcgcgatg atcgcttcga cgatcaactg cgccacgcgc tgccccgctt
     5881 caatcgtgca cgcttcgcgc ccgtgattga cgagcgcgca gagaatctcg ccacgatagt
     5941 cgctgtcaat aactccggcc aaaacgtcga ggccgtagtt tacagccaga cccgagcgcg
     6001 gcgcgacccg cccgtaaaat ccttcgggaa tcgcaacggc caagcccgtc cgcgccgccg
     6061 tgcgcgcccc cggctcaagc gtcagggttt caatcgcata gagatcgagt cccgccgcct
     6121 gacggcttcc gcgcgtcggc aacttcgccg ccggatgcag gcgcaaaaat ttcaactcat
     6181 cggtcatgtg aaagctaaag aatacagaag tcagaataca gaagtcagaa tttagaagtc
     6241 agaagtcagc ataaaagagc gttccggctt tccttccgac tccggtgttc tgagttctgt
     6301 attctccttc acgcccgcgc ttcgttgatc cagacgggcg gggcgttgca gccgctcttg
     6361 tcgctcttcc agcgcgtgca gccccagaag tagctgtcgt tgcgaaaggg tcctcgcttc
     6421 accgtcggcg cgccgcacgc ctggcacggc tggccgctct ccggcagggc tttcgcctct
     6481 tcgagcgcgc cctcctcgtc gaaccagcgc acgcccctgc attccgggta acgcgcacac
     6541 gagagagacg cgcgtttgcc cttctcacgc ggcgcgcgca ggcgcatcgc gccgccgcac
     6601 acggcgcact tcgtcgccag tagaattttg cgctgcttcg cgttcttcgg cgcgtcgctg
     6661 atgaagccgc acgcgtcgcg cccggcggag cagacgagaa actttccgtt cgcgctcacg
     6721 cgttcgacga gaaatcccgc gcgctcttcc agtttgcact tcgggcaatc cggcgagtct
     6781 gcgggacgct ccacgtccgc gcgcttcgcc acttgatcgc ccgtcgcgcg ttccatctcc
     6841 gaggccgcga tctgcccgac gagttctctc accatctcct tcacctcgcc catgaacgca
     6901 tcgcgcgcat actcgccgcg cgccattcgc gccaacttct gctcccaact gccggtcagt
     6961 tcggcagact tgagaagttg cgaaggcagg agcttgatga gggcgatgcc cttcggcgtc
     7021 gccgtcagag atttcttatc gcgcgtgatg tactcgcgtt tcaagagcgt ctcgatgatc
     7081 tgcgcgcgcg tggcgggcgt gccgagtccc gaatccttca tcgccaagcg caactcctcg
     7141 tcttcaacct tctttcccgc cgtctccatc gcgccgagca ggctcgactc cgaataacgc
     7201 ggcggcgctt tcgtctgctt cgccaccgcc tccaccttcg tcgtttcgac ggactctttc
     7261 gcacgcacct gcggcaactc cgcgtcttcc gcttcctctt cgccgtccgt cttcttccgc
     7321 tcttccctgc ccggtgggtc aacttcgcgc cagcccgcct tgagcaccac cgtcccgcgc
     7381 gtgatgaact gttcgccgtc aacggcggtc gtgatcgtcg tgcgttcgag ttccgcgtcg
     7441 gggtagaagg ccgcgaggaa gcggcgcgcg atcagatcgt agatgcgctt ttcgtcaggt
     7501 tgcaaagcgt tcggctcgac gcgttgtttc gtcgggatga cggcgtggtg atccgtcacc
     7561 ttcttgtcgt caacgtggcg gctcgtgagc ttcggcgttc ccttcgccag aatcgtctcg
     7621 atgaacttga cgtaaggacc aacgctcgcc gcttcgacgt gcgctttgag ttcctgattg
     7681 acgctcgtgg agagatggcg cgaagaggtg cgcgggtagg tgatgaactt tttctcgtag
     7741 agcgtctgcg cgagttcgag cgtgcgcgcg gcggagaaac tgtagcgggc gttcgcggcg
     7801 cgctggagcg ccgtgagatc gtagagcagc ggcgcgcgtt ccttcgaagc cttcttctca
     7861 actttttcga ccgtgcccgg cttgccctga actttgaaga cgacggcttc ggcagcctct
     7921 ttgtgatcga agcggctgcc ctgtttgttg aaccacgtcc cgcgatacgc gcccgccgcc
     7981 gactgaaaat cagcgaccac ctcgtaatag ttcgtcggga caaaattcgt gatctccgcg
     8041 tcgcgcgcga cgatgagagc gagggtcggc gtctgcacgc gtcccagaga atagacgccg
     8101 tcgtagcccg cgccgcgcgc cttgatggtc tgcgcgcgcg tggcattgag gccgacgagc
     8161 cagtcggcct gttgccgcga gcgcgcggaa tcacgtagcc cccggtagtc tgcggcgggc
     8221 ttgaggcggt tgaagcccgc gacgatctcg tcgcgcgtga gggaggaaat ccagagccgc
     8281 tcgaccggct tgcgcgagcg cgcgagcgtg tagacgagat cgaagatcaa ctcgccttcg
     8341 cgcccggcgt ccgccgcatt gacgatgctc gtcacgtctg cgcgcttgag cagattcttg
     8401 atcacgttga actgatcttt cgtccgctcc gaggcgtgat atttgaactc ttcggggatg
     8461 atgggcagcg tctccatccg ccacgatttc aacttcggat cgtaagtttc cgggtcgtcg
     8521 agttcgacga gatggccgat gcaccaggtg atgatgtcgt ctttgccttc gaggtagcct
     8581 tcgccgcgcc gcgtcgcgcc gagcgcggcg gccacgtcgc gccccatcga aggtttttcc
     8641 gtgatgatga gtctcattcc gagtaataat ctactttgcc ttcggcggtt ctctgaaaat
     8701 gaacgccgta cctgccttca taagcgtcgg cgcagccgtg actacagaac catccgaaat
     8761 taggaatggg gactttacct tctgccagta gttgctcaca aggcggcgcg tgcgtgtcgg
     8821 aagaagcatc gtaacgactt aacaacttcc cgcacatcgc acaaccgatc ccgtccgggt
     8881 ggagattttt gcgcgcagta atgatgaacg cgccgacctg ctctctacgc cccctgtccc
     8941 tgatccggcg ggtaaacatt aagctcttca acttcatcag ccacgagttc atccctctgc
     9001 cctcatcctt gatcagagtc ccgccatgtc ccacttgcgc acgacatcgt tgacgagcag
     9061 ggcgatcttg tccgtcactt tgacgaaata ctcgtcggcc ttcttctggt cgaacttcgg
     9121 atagccctgc cccggctgat agaggccgat ggaagaggga aaaggggtgg ccgaccaggt
     9181 gcccgccgca ggatagggcg tggcgaggtc gtccttgtag cgttcggggt gaacgagcgt
     9241 cgggtcaatc gcctgcacga aggccgtctc gttccaaccg gcgtggccgc cgtcttcgcc
     9301 gaagacctgc ttcgtgacat ccgaggcgaa cgaccaccag ttgacgacga gcgtgcgcac
     9361 gcgtctctcg gcggcgactt ccgccgcgac ggaattcagg acggcggttt gcccgccgcc
     9421 gtgaccgttg atgatgatga tattgcgaaa accattcttc gcgagtcctt ccaagatctg
     9481 tttgacgaac gggcgatagg ccgcttccgt aatttgaaac gcgcccgggt aagcctccat
     9541 gctgcccgtc atcccgtaag gcaggacggg cgcgatcatg gcgttcgtgc gttgcgcgat
     9601 gcggcgcgcg atggcgagcg gcgcggtgtt gtctgcgccg ttgttggcga cgccgtgcgg
     9661 ttcgagcgtc cccgtcggca agagcacggt ctggacgcgc gacgggacaa cttcgcgaaa
     9721 ctccatccag ttgatgcgat ccatctcgcg cgtcgagacg gtgtcggcta gggggcgcgc
     9781 ggcgttcgtc gtctgcgccc gtacggggag cagcgcggag gctccggcaa acagcaaaag
     9841 tagaaagatc gtctgcgcta ttctcatcgt ttcatttcct cgattgattt acacactccc
     9901 ggcgtgaagg tttccgcact ttgagccgcg tgttgaattt tagcctatca caccgaacca
     9961 atcgtacaaa tccaacaact cgccgggcgc gaacgagagc agaccggcct ctcgcgcgcg
    10021 tctccggcgg gcgcgagagg ccggttgcgg agcgttctgt ctaaagatat attctccttc
    10081 gtccaacgcc gtctacggag cagagattcg ccgacaggct gcaccgtcca actcgtttcc
    10141 aaattgatat tcaacgtccc gtccgggagg agagagcctt tgagccacag taaagaagag
    10201 gcgcaacaaa cttcgagcct caatcgcaga gaatttctcg gaacgactct taccgccagc
    10261 atcgccgccg cgacgactct ggcgcatccc gcaccggcac gcgccgagcc tctcgctcct
    10321 gacgcttctc cgttgacgaa cgcgccgcgc gctttcgaac tcgaagagac gaccatcgcc
    10381 gccttgcagg aagcgatgcg cgaaggaaaa ttgacggcgc gcgagatcgc cgggcggtac
    10441 atcgaacgca tcgaaaaatt cgaccgcaaa ggcgcgcggc tcaattccat catcgaactc
    10501 aaccccgacg cgctcgcgct cgccgccgcg ctcgaccgcg aacgcaagac caccggcgca
    10561 cgctcgccgc tgcacggcat tcccatcctc ctcaaagaca acatagatac cgccgaccgc
    10621 atgcgcacca ccgccggttc gctcgccctc gccgacactt tcgcaccgcg cgacgccttc
    10681 atcgtcgagc gtctgcgcgc ggcgggcgcg gttctactcg gcaagacgaa cttgagcgag
    10741 tgggcaaact ttcgttcgac gcattcgtcg agcggttgga gcgcgcgcgg cgggcagacg
    10801 cgcaatcctt acgccattga ccgcaacccg tgcggttcga gttcgggttc gggcgtggcc
    10861 gttgctgcca acttcgccgc cgccgctatc ggcacggaga cggacggctc aatcgtctgt
    10921 cccgcttcgg cctgttcgct cgtcggcctc aagcccacgc tcggcctcgt cagccgttcc
    10981 ggcatcatcc ccatcgcgca cagtcaggac accgccggtc cgatgacgcg caccgtcgca
    11041 gacgccgccg ccctcttgaa cacgctcgcg ggaatcgacc cgcgcgaccg cgcgacgcaa
    11101 acctcgcgcg gcaagtcgca cgcagactac acgcgtttcc tcgaccccgc aggtttgcgc
    11161 ggcgcacgca tcggcgtagc gcgcaagttc ttcggcttta acgacaaggt tgacaagttg
    11221 atggccgacg cgattgacgc gttgaaacgc gcgggcgcga caatcgttga cccgactgat
    11281 ctgccgtcgc acggcaaata cgacgattcg gagtttgaag tgttgctcta cgagttcaag
    11341 gccgacctca acaagtatct ggcggagcgc ggcgggggcg cgccccgttc gctcaaagaa
    11401 ctcatagact tcaacgagcg caaccgcgag cgcgagatgc cttacttcgg tcaggagatc
    11461 ttcctcaagg cgcaggcgaa aggagcactg acgagcaaag cctatctcgc cgcgctcgcc
    11521 aaaaatcatc gcctctcccg cgccggaggc atcgacgccg ccctcaccaa acatcgtctc
    11581 gacgcgctca tcgcgcccac tggcggacca ccctggacga ccgatctcat caacggcgac
    11641 catttttccg gcggcagttc cacgcccgcc gccgtcgccg gatacccgaa catcagcgtc
    11701 ccggcgggct acgtcttcgg cctgcccgtc ggcatctcct tcttcggcac ggcctacagc
    11761 gaacccgtgc tcatcaaact cgcctacgct ttcgagcagg ccaccagaca ccgccgcccg
    11821 ccgcagttta cagacagcgt tgatttgcgc gattaggagt tgacgaccaa acgacgaact
    11881 atgaatcgtc cctgaactat gaatcgttcc cgtgcttctt tcgctttaac tattcattca
    11941 aactctacgc ggcggagcac gccttcctgc gcgacgaagg cgcacgctgc gacctgcaag
    12001 cgaccggcga ggcttccggc gaaatgctcg ctctctcccg gcgcgctttt gcgccatagc
    12061 tcgcagccgc acgagagcct cctgagtctg catttgaagt atggcgcgaa cgggtgttag
    12121 gttgaggcgt gtcgccggaa gttaaacaca cattgacggc gtggctgccc gacctcgtct
    12181 acacgggcgg cgggcgattt gagagcggcc tcgcgctcgt ctcagaccgc gcgggacgca
    12241 tcacgcatct cacgcgcgac gttgaaagca ccggcgcagc gcgcatcgtg cgcttgcagg
    12301 gtcgcgcgct gttgcccggc ctcgtcaacg cgcactcgca cgccttccaa cgcgtcatac
    12361 gcgggcgcac cgaacgccgc tctgccgcgc atgattcttt ctggacgtgg cgcgagatga
    12421 tgtacgccgc cgccacgcgc ctgtcgcccg atgatctcta cgacgcctcg cgcatggctt
    12481 ttctcgaaat ggccgcgagc ggaatctccg cggtcggcga gtttcactac ctgcaccaca
    12541 cgcccgaagg cacgcgctac gcggacgaaa acgagttggc aaaacaggtc gtccgcgccg
    12601 cacgcgacgt gggcttgcgc atcgcgttgc tgcgcgtcgc ctacgcgcgc gcgggttgga
    12661 acacgccccc caacacgaga caggcgcgct tcatcgaaca cgacgcgcag acgtttctca
    12721 ggcagacgga gactctcaaa aaagatattg cccgccagat gtcaaacgcg gtcgaagcct
    12781 caaacgctgt cgaggcgtcg aacgatgtag acgcgcggga agagtcggaa gcggtcggcc
    12841 tcgacgccag cgcaagcagc acgtgggaga gcgcaagcag cacgcgggag agcgcaagcg
    12901 aagcgtgggt cggcgtcgct ccgcatagcg tgcgcgccgt gccgctcgac tacttgcgcg
    12961 aggtgaatgc gtttgccgtc gcgcacgacc tgcccgtcca catgcacatc gcggaacagc
    13021 ccgccgaggt cgccgcctgt ctggcagaac acgggcgcac acccgtcgct ctgctcgcgg
    13081 acgaaggcat actcggcgcg cgcttcaccg ccgtccacgc cgtccacatc acggcggaag
    13141 aggcacgcgc gctcgccgca gagcgcgcac acgtctgcgc ctgcccgact accgaacgaa
    13201 acctgggcga cggcatcatc cccgccgacc tgctgctcgg cgcgggcgtc cgcgtctcgt
    13261 tcggcacgga cagccacacg cagatcgatc tcctcgaaga tgcgcgcgaa ctcgaatacc
    13321 acctgcgcct gcaaaaactc gaacgcgccg tgctcgcccc cgtaacgact ggcgcacaca
    13381 cgacggagac gaacgccgca cgcgccgaca cgcacgaagc cgacatggag acggacagcc
    13441 tcgccgccct cgccgcgcgc ctcttcgagt gcgcgacgac gaacggcgcg tcaagcatcg
    13501 gcgcacccgg cggcagccgc aacggcagcg tcgcgcccgt tgaggggagc aacggcggcg
    13561 cgttcgaaat cggcggcgtc gcggatttct tcaccgtcgc cttagacgat ctctccatcg
    13621 ccggagcggg aacggaagac ctcttacccg ccatcgtctt ctcgctcgcg cggacagccg
    13681 tgcgcgatac ggtcgtcggc ggtcggcgca tcctcgaaga tggacagcac cgcgcgcaag
    13741 atgagatcat cgaacgcttc acccgtgtgc agcgcaaact gtggcgataa acgacgcccg
    13801 cccggcgcac ccgcctctac gaagtcagga gcgcacgatg acgaacgaag agatgcaacg
    13861 aattatggaa ttcacgatgc agcggcaaga ggctttcacg gagcaacagg agaagttgag
    13921 cgcgtcgccg gacagactcg aacaagagcg tgttgccgag actgtaagaa agggtttatg
    13981 agtgttgccg agacgttagc ggagttagtt gctattgact ctgtttcatc ccgctcaaac
    14041 ttggaaatta ttaagtttct cgctggacgc gccgaagcgg ctggcttgtg cgcccgcatg
    14101 ttcccttaca cggatgcggc gggagtgaag aagttcaacc tcgtctgtgt cccgccgagt
    14161 gtgaacgctg aacgaatgga agttgaactc gcgctcgtgg gacatacgga tacagtgcct
    14221 tacgatcctg cgtggacgga cgcgctcacg ctcagcgtgc gcgaggggaa gttttacggg
    14281 cgcggctctt gcgacacgaa aggtttcatt gccgctgcgc tcacggctat cgagcaagtc
    14341 gatctcgcac gtctgtcgaa accgctcgcg ttcgtcttca cggcggacga agaggtcggg
    14401 tgcatcggcg cgaaacatct ggcggagtcg cgtgcgctcg cggcgcgtta cgccatcgtc
    14461 ggcgagccga cttcgttgca gccgatgcgg gcggggaaag gttattgtct cgccgaggtg
    14521 cgggtcggcg ggcgcgaagg gcacagcgcg tatcctgcgc tcggcgcgtc ggccatcttt
    14581 cgtgcggcgc ggctcatcgc gcgcatcgag ggggtggcgg agaaattaca ggccgagcct
    14641 cacgcctcgt tcgatccgcc ttacacgacg ctcaacgtcg gcgtgattcg cggcggcacg
    14701 gccaaaaaca tcatcgcggg cgagtgtcgc ttcacgctcg aatggcgtcc cgttcccggt
    14761 caggcagcca cacacacggt tgaactcatc caaaacgaga tcgagagttt gcgcggcgtt
    14821 gaaccggact ttgactgcga agtcgaaatc ctccggctcg acgaagcatc ggagacacgc
    14881 ccggactctt tgctcgtcaa cgaattggag actttgagcg ggcaggcggc gggcaccatc
    14941 gccttcggca cggaagctcc gcaactgaca gagatgggcg cggaggcagt cgtcttcgga
    15001 cccggcagca ttcgcaacgc gcatcgcacg ggagagtttg tcccggcgga cgaactcgcc
    15061 gcatgcgtcg aaattctcgc gcgggcgata gagagatttt gccggtagtt ggttgtgcgt
    15121 tgccggtaaa cgtctgctcg tgcttaatga cagaaatggc agcgcggcca gagattgata
    15181 tgaacggctg gcggctgata gctgcaagcc ggtggctttt tatgaaccaa agaattttca
    15241 cctccgcctc ttttcggacg atgctcggcg cgtcgctgct tatcgttctc tccgtcattc
    15301 acgtcgcggc gcaggcgcag gcgcaacagc agaaacctct gacgagtcag gaactcgtgc
    15361 ggctcgtcta tcaactgccg tcgcagccgc agaaacgcga cgagatcatc gaggagatac
    15421 gcaggcgcgg catcggtttc gagttgacga cgggaatccg cagcatcgtg gcgaccaaga
    15481 gcggcaacga tgcgcttttg cggcgcacgc tggaagaggc cgcgcggcgg cgtctgaacc
    15541 cggcgggcgc ggcggcgcgt ccatcggagc gggaagcgga ggacgcgctg gagcggacgc
    15601 gtaaggcgac gcgcgaggcg gcgggcgcga tgcctgattt cgtcgtccgc caactcgtgc
    15661 ggcgttcgtt ggcgcgcggc gagacgcgca acttttcgca gagagatcaa ctgaccgtcg
    15721 ccgtcagcta tcgcgagagc gcgggcggcg agcagtacaa acttctggcc gtcaacggtc
    15781 tgcccgtcac cgaagagtcg agggagcgcg gcagctacga gcaggcgggg ggcacaagtt
    15841 cgaccggcga gttcgtctcc acgctcaacg agcttttcgc cgatcatacc aagaccgaat
    15901 ttcacgccgt tgacaccgac accatcaacg ggcggcgtgc catcatctac gactacgcgg
    15961 tgaagatcga caactccaag cagacgctca aggtaacgga atcgcgcggc atggagccgc
    16021 tcgtggcgcg cgtcggctat cgcgggcgcg tctggatcga ccgggaggct ttccgcgtcc
    16081 tgcgcatcga agtgatctcc acggacatcc cctacgactt ccccatcacc gccgccgtca
    16141 acacgattga ctacgattgg gtggagatca gcgggcgcaa atacctgctc cccatccgct
    16201 ccgaagtcga actcacgacg cgttaccagc agcaaactta ccagacgcgc aacgagatac
    16261 gcttccgcaa ctatcaacgc ttcggcagc
//
LOCUS       DASWNS010000274         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_219316, whole genome shotgun sequence.
ACCESSION   DASWNS010000274 DASWNS010000000
VERSION     DASWNS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219316"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1657)
                     /locus_tag="VGB76_09740"
     CDS             complement(80..1657)
                     /locus_tag="VGB76_09740"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY0099224.1"
                     /translation="MNLPLAFQTAQAVSPDINFSLILPEAIVSVAGVLVMLVDAFTRR
                     SNQRWVTGLLSLFGLAAAGVACLWLWNDETTAREAFNGMIVLDRMRLSFTLVFLVVSV
                     LTVLISMIWVEWEHLPAGEFHALLMFATVGMMLMASGGDLVIIFLGLELLSIATYVMA
                     GFRRTDLRSNESSMKYFILGSFSSAFLLYGIALVYGGTTLANGGAGTTNIKELALRIG
                     AENALAYPALLYAGAALMLVGFGFKIATAPFHVWTPDVYEGAPTPVTAFMAAGPKASG
                     FAAFMRVFLFGFPFVAATTNAAQLHSAWINAIWLLAILTMCVGNLAALVQTNVKRMLA
                     YSSIAHAGYALVGFVAAGQATDKTLQNDAIAAVAFYLLAYAVMNLGAFAVVTLIARSG
                     DRRTEIEDYNGIGFTAPALSFALTLFLLSLLGIPLTAGFMGKVMVFRAALDQGFVALV
                     VIGVLNTAISVYYYLRLIVVMFFRERSTAWSAPAVPVSVALAIIITALGTFYLGIFPG
                     QVLDAFRSEQTVVSQLK"
     gene            complement(1730..2308)
                     /locus_tag="VGB76_09745"
     CDS             complement(1730..2308)
                     /locus_tag="VGB76_09745"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0099225.1"
                     /translation="MWKLNKRFFLPVTLVVLLLCGGAWVTAESRREPDDAQVSIKLKG
                     VDGKTYDIAAMRGEVVLVSFGATWCKPCAWELEALEDLKREYAGRGVRFLWVSIENDD
                     QTSNELLRDYAKSLKMTIPVLRDPEKTAYAQFSERVRLPMVVFFDREGKFVAPKHTGM
                     TSQAEEYKKLIRSRLDALLSTKEETGGAGRLR"
     gene            complement(2356..>2833)
                     /locus_tag="VGB76_09750"
     CDS             complement(2356..>2833)
                     /locus_tag="VGB76_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY0099226.1"
                     /translation="HYERRHTRMISEFGGLAKPMPWFATLFVFASLSSVGLPILNGFV
                     GEFLIMLGMWTSPALSTGWARLATMLAATGVIWAAVYMLWMLQRVLFGRVRNEQNARL
                     TDLNSRELGLLVPLLVLMIFMGVYPRPFLNRSRAAVELVRSRVVATEGGGSIAESR"
BASE COUNT          621 a          858 c          876 g          478 t
ORIGIN      
        1 acgaagacag gttttctcta ttcacgattt acgattcacg attcactttc ttctcttgtc
       61 acttgtcaca cgccgctcgt cacttcaact gactgacgac ggtttgctcg gagcggaagg
      121 catcgagcac ctgaccggga aaaattccga gataaaaagt gccgagcgcc gtgatgataa
      181 ttgccagcgc gacgctgacg ggcacggcgg gcgcgctcca cgcggtcgag cgttcgcgga
      241 agaacatcac gacgatgagg cgcaggtaat aatataccga gatcgccgtg ttgagcacac
      301 cgatgactac gagcgcgacg aagccctgat cgagcgcggc gcgaaagacc atcaccttgc
      361 ccatgaatcc ggctgtcagc gggatgccga gcaggctcag cagaaagagc gtgagcgcga
      421 aggagagcgc gggggcggtg aagccgatgc cgttgtagtc ttcaatctcc gtgcggcggt
      481 cgccgctgcg cgcgatgagc gtgacgacgg cgaacgcgcc gaggttcatc acggcatagg
      541 ccagcaggta gaaagcgacg gcggcgatgg catcgttttg cagagtctta tccgtggcct
      601 gacctgccgc gacgaagccg acgagcgcgt agcccgcgtg cgcaatggac gaataagcga
      661 gcatgcgttt cacattcgtc tgcacgagcg cggcgagatt gccgacgcac atcgtcagga
      721 tcgcgagcag ccagatggca ttgatccagg cggagtgtag ttgcgcggcg ttcgtcgtgg
      781 cggcgacgaa ggggaagccg aagaggaaga cgcgcatgaa agcggcgaag ccggaagcct
      841 taggtccggc ggccatgaaa gccgtcacgg gcgtcggcgc gccctcgtac acgtcgggcg
      901 tccagacgtg gaagggcgcg gtcgcgatct tgaagccgaa gccgacgagc atcagagccg
      961 cgcccgcgta gagcagcgcg ggataggcga gcgcgttctc cgcgccgatg cggagcgcga
     1021 gttctttaat gttggttgtg cccgcgccac cgttggcgag cgtggtgccg ccatagacga
     1081 gcgcgatgcc gtagagcaga aacgccgacg agaacgagcc gaggatgaaa tacttcatcg
     1141 aggattcatt cgagcgcaag tccgtgcggc gaaatccggc catcacgtag gtggcgatgg
     1201 agagcagttc gaggccgagg aagatgatca cgaggtcgcc gcccgaggcc atcaacatca
     1261 tcccgacggt ggcgaacatc agcagcgcat gaaactctcc ggcgggcaag tgttcccatt
     1321 caacccagat catcgagatg agcaccgtga gcacggagac gacgaggaag acgagcgtga
     1381 aggagagccg catgcggtcg agcacgatca tgccgttgaa ggcttcgcgc gccgtcgtct
     1441 cgtcgttcca cagccagaga caggcgacgc ccgctgcggc gagtccgaag agcgagagca
     1501 gccccgtcac ccaacgctgg ttcgagcggc gcgtgaacgc atccacgagc atcacgagca
     1561 cgcccgcgac ggacacaatc gcttccggca ggatgagcga gaagttgatg tcaggcgaca
     1621 cggcctgagc agtttgaaag gcgagaggca agttcatctt tttaacccgg agcgaacgac
     1681 tattaactag ccgtcaagtt taaatggcga ccggcggcgg caatgttttt cagcgcaggc
     1741 gtcccgcgcc gcccgtctct tctttcgtgc tgaggagcgc gtcgagccgg ctgcggatca
     1801 gctttttgta ctcttcggcc tgcgaagtca tgcccgtgtg tttcggcgcg acgaactttc
     1861 cctcccggtc gaaaaagacg accatcggca ggcgcacgcg ctcgctgaac tgcgcataag
     1921 ccgtcttctc cggatcgcgc agcacgggaa tcgtcatctt caaagacttc gcgtagtcgc
     1981 gcaaaagttc gtttgaagtt tgatcgtcgt tctcgatgct cacccataga aacctgacgc
     2041 cgcgccccgc atactctctc ttcaaatctt ccagagcttc gagttcccac gcgcagggct
     2101 tgcaccacgt tgcgccgaac gagacgagca ccacttcgcc acgcatcgcg gcgatgtcat
     2161 atgtcttacc gtcaacgcct ttcagcttga tgctgacctg cgcatcgtct ggctcgcggc
     2221 gcgactcagc cgtcacccac gcgccaccgc agagcaggag cacgaccaac gtaaccggaa
     2281 gaaagaatcg cttgtttaat ttccacatac ttttgacgat cccgtattta cacgtccacg
     2341 cgcccggctt tgccttcaac ggctctcggc gatgctgccg ccgccttcag tcgcgacgac
     2401 gcgcgagcgt acaagttcga ccgcggcgcg cgagcgattc agaaagggtc gcggatagac
     2461 gcccatgaaa atcatcagca cgaggagcgg cacgagcaag ccgagttcgc gcgaattgag
     2521 atcggtgagc cgcgcgttct gctcgttccg cacgcgcccg aagagcacgc gctgcaacat
     2581 ccagagcatg tagaccgccg cccagatcac gcccgtcgcc gccagcatcg tggcgaggcg
     2641 cgcccacccc gtagagagcg cgggcgaagt ccacatgccg agcatgatca aaaactcgcc
     2701 gacgaagccg ttcaagatcg gcaagcccac ggacgacagc gaggcgaaga caaagagcgt
     2761 agcaaaccac ggcatcggtt tcgctaagcc cccaaactcc gagatcatac gcgtgtgccg
     2821 tctctcgtag tgc
//
LOCUS       DASWNS010000275         6069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_479694, whole genome shotgun sequence.
ACCESSION   DASWNS010000275 DASWNS010000000
VERSION     DASWNS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479694"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="VGB76_09755"
     CDS             complement(<1..1201)
                     /locus_tag="VGB76_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY0099227.1"
                     /translation="MNISRRDLIKGLLATSAIVGFDLAGRSWVTQASSGVGFAQLPPL
                     DGTLTTDPAALAAAADDFGHIIHSTPVAVLRPGSVADIVKVVRFAREHGIRIAGRGKG
                     HTAFGQSQAGAGVVIDMSTLNQIHAIEPDRAVVDAGVVWRDLLLATIPAGLTPPVLTD
                     YTRLTVAGTLSVGGVSGRSYLHGAQVDNVLELQVVTGEGELVTCSESVNKSLFDAALA
                     GLGLCAVIVRATIRLIPAGERAQTLRFFYPDVPSMLADLRFLTRAERFDHIRGMSVAT
                     PDGFAFFIEGTSFYTSSGELPANPTQGLNYIPGSQQLEDMTYFEYTDLVVKLIDQLNE
                     AGLGGFPHPWLDMFVPDSKIDDFASQTIASLDPSLFLPGSLMLFFPFVKSRLTRPMFS
                     VPDEETFF"
     gene            complement(1465..1596)
                     /locus_tag="VGB76_09760"
     CDS             complement(1465..1596)
                     /locus_tag="VGB76_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099228.1"
                     /translation="MERVGLVVAVEANSADVRLRAGDLPGGVEKADVPVGTQVWTPE"
     gene            1874..2284
                     /locus_tag="VGB76_09765"
     CDS             1874..2284
                     /locus_tag="VGB76_09765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEY0099229.1"
                     /translation="MSKGKQLYAVKRAATGLGLFAVETIPARKRIIEYVGTLISNEEV
                     EKSRGKYFFGVNTKWAIDGSARSNIARYINHSCSPNAEAFISGRRVWVWSKRKIKAGE
                     EITIDYGKEYFEDQIKPRGCRCAVCSNKSRRVSA"
     gene            complement(2281..2496)
                     /locus_tag="VGB76_09770"
     CDS             complement(2281..2496)
                     /locus_tag="VGB76_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099230.1"
                     /translation="MADEIAATAGEIMLLLADHGEDELVCVTLKDARRLREVARTLQP
                     RRAQVVEVGVALTNDVALIINAEKRAA"
     gene            complement(2737..3081)
                     /locus_tag="VGB76_09775"
     CDS             complement(2737..3081)
                     /locus_tag="VGB76_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099231.1"
                     /translation="MRGTVQPPRRDTYIFHARQGQVIVTDVLYHGNTIRRRTDDEEGS
                     SDFIFIHPDGERVEDAQDVVFSARQTGDYRVIVRPTRRRTNPRYTLKISLHPSNWRDI
                     VDFSDSSRQRCP"
     gene            complement(3347..>6069)
                     /locus_tag="VGB76_09780"
     CDS             complement(3347..>6069)
                     /locus_tag="VGB76_09780"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEY0099232.1"
                     /translation="SLIFSGYPLLSMGQLSLGYPFTWGYLFLPGVWAEQVYVLSPFLF
                     APAFLYAYIRELGLSRIAALLAGLGFAYSGLTTNVLGHNGLTSNAVMWLPLLLLFIER
                     ARTRTLATCVLWGGLAYAMSVLNGYGQGFLYVAIIAVAYACFLCLAPPRGRLPHADSE
                     RPRWFSAARWRPLFAAICAVVAGAGVAAFQVLESMRAVRFSIRSVLSFELFNSGFFTP
                     QTILKSLLFPAYYELEVTTYMPLLAVVLAGAGGVFAMRRGRDARVIFWLATALAAWAL
                     MLGDYTPLNRLLYNVPLVNLFRRPARHMFEWSFAVSVLSAYGWDALAAWHARRTVQSP
                     RLKFNISLITLMLAALSGLIWWAQLAPAPFPNHALNKGLVQSSYAGWKVAFTILTFVS
                     IWTGWQIRKGRWRTGQLLGAVLLTCFMEPFILLSVWWLPWARPADQFAPPSVAGGFLR
                     NHDPVQNRAYLRVDLFADQFSERPRVDAPNLSMLAGIHNVAGYEPLIFERYSRALGNV
                     GMDGFNPRPGFPSDLSIFGNNSHVLDLLNTKFFVSFSDFSAAPITRLEQAGISYSGVE
                     RGRHIEAGRKALIHGRSAQGDTLAIVSSLANSSNNADGEVVAKLRILTSDGQTLEREL
                     RAGTDTADGAHERTEVRGNIRHSLAPVFDSRPGDAPDTFTAHRYRASIPLGRRAAVEQ
                     IEIENVAAGASLALWKTTIFDSETRRSTTLAHAGTRLPVELNPGKWQTVYDADDILIL
                     RNERALPRAWLVAEAEAVDGEEALRRIRGESDKEFDPRRTALLEVSTGELPALPGGLV
                     GSNATAHVSYQPNGVAVDTYAETDALLVFSEINYPGWTATVDGIERPIYTTNFLLRGV
                     HVPPGAHRVEMKYAAPSARTGAWISLATLLLLTSLALLGRRPAKALRRIL"
BASE COUNT         1281 a         1725 c         1915 g         1148 t
ORIGIN      
        1 ggaagaaggt ctcttcgtcg ggcacgctga acatcgggcg ggtgagccgc gatttgacga
       61 acgggaagaa aagcatcagc gagcccggca ggaagagcga agggtcgagc gacgcgatgg
      121 tctgcgacgc gaagtcgtcg atcttcgagt cggggacgaa catgtccagc caggggtgcg
      181 ggaagccgcc aagccccgct tcgttcaatt ggtctatgag cttcacgacg aggtcggtgt
      241 actcgaagta ggtcatgtct tcgagttgct gcgagccggg gatgtagttg agtccctgtg
      301 tgggattcgc aggcagctcc ccggacgacg tgtagaagga cgtgccctcg atgaagaagg
      361 cgaagccgtc gggcgtggcg acgctcatgc cgcgtatgtg atcgaagcgt tccgcgcggg
      421 tgagaaatcg aaggtcggcg agcatcgaag gcacatccgg gtagaagaag cggagcgtct
      481 gcgctctttc cccagctggg atgagacgga tcgttgcgcg gacgataact gcgcacagtc
      541 cgagtcccgc cagcgcggca tcgaagaggc tcttgttcac cgactcggag caggtgacga
      601 gttccccctc ccccgtgacc acctgaagtt cgaggacgtt gtccacctgc gcgccgtgca
      661 ggtaactcct gccgctgacg ccgcccacgg agagcgtccc cgccacggtc agacgcgtgt
      721 agtctgtaag caccgggggc gtgaggccgg cgggaatggt ggcgagcaag aggtcgcgcc
      781 acacgacgcc ggcgtcaacg acggcgcggt caggctcgat ggcgtgaatc tgattcagag
      841 tgctcatgtc tatgacgacg cccgcgcccg cctgcgactg gccgaacgcc gtatgccctt
      901 tgccacgccc ggcgatgcgt atgccgtgtt cgcgagcgaa gcgcaccacc ttgacgatgt
      961 ctgcaaccga gccggggcgc aggactgcga cgggtgtgct gtgaatgatg tggccgaaat
     1021 cgtctgcggc tgcggcgagc gcggcggggt cagtggtgag tgtcccgtcg agtggcggca
     1081 actgtgcgaa gccgacgccg ctgctcgcct gcgttaccca tgagcggcct gcgaggtcga
     1141 atccgacgat agccgaagtt gctaacagtc cttttatcag atcgcgtctg gaaatattca
     1201 tcacaagtta cctctcttcg tgagctttac ggaacctcaa gcctgacatt tgcgccttgc
     1261 tgttgcgagc caccttcgcg tgaatgatct agttcaccgg acgacgtagc agtggtgggt
     1321 gatgcgccgg gcgtctcatc tatataacgc gagagtcgta cgcaaaaggg atcatatcgc
     1381 agatattttt tttcaggtaa gacgccagca gagaagtctc acctccggag tttacccttc
     1441 ccatgacggc accgcaaatc gcctctactc cggcgtccat acctgcgtcc ctaccggcac
     1501 gtcggccttc tccacgcctc ccggcaggtc accggcgcgc agccgcacgt ctgcggagtt
     1561 cgcctccacg gcgacgacca acccgacccg ctccatccgg aaggtgtcta tgatctttaa
     1621 agagtaactg actcataact aatcagagcc tacgtaaggg caagcggcac agcgcacggc
     1681 aacgcccgtc cgcacactac tgccgaacca ttctctcttg cggacggcat tctcgctcct
     1741 tgcgggtcgg gagcctgccg tcgttataac cttctcataa attctatatc tatcaagtta
     1801 aaagtgagat gagttgacgc ttgaattcaa accttatttc gtagcctggg gaatatattg
     1861 ttaccatcac aatatgagca aagggaagca gctttacgcc gtcaagcgtg ccgcgacggg
     1921 attgggatta ttcgccgtcg agactattcc ggcgcgtaaa cgaattattg agtatgtcgg
     1981 cacgctcatc tcaaacgaag aggtggagaa gagtcgcggg aaatattttt tcggcgtcaa
     2041 cacgaagtgg gcaattgacg gcagcgcgcg ctcaaacata gcacgctaca tcaaccactc
     2101 gtgtagtcct aacgcggagg cttttatctc cgggcgacgg gtttgggtct ggtcgaagag
     2161 gaaaatcaaa gcgggcgaag agattaccat tgattacggc aaggaatatt tcgaggatca
     2221 gattaagccg aggggttgcc gttgtgctgt gtgtagcaac aaaagcagga gagtctctgc
     2281 ttaagctgca cgtttctctg cattgatgat gagcgccacg tcgttcgtca acgccacgcc
     2341 aacctctact acctgagcgc gccgtggctg gagcgtgcgt gcgacctcgc gtaaccgtcg
     2401 agcgtctttg agggtgacac acaccagttc atcctcgccg tggtcagcca acaacagcat
     2461 gatctcgccc gccgtggccg caatctcatc cgccaactcc ggagatgcct cgtcctgctc
     2521 attgccgaga aggagagagc ataattcttc gaggcgggtt gagaagcgag agagtttgtc
     2581 taactgaaaa ttgtgatgac tcatggtgag acgcgattct atcatcaagc gtctgacgag
     2641 gctagtatta tttgtgcgac tttgtgccgc ccgttgtaca cagggcgatt gagatgccgg
     2701 gacgttattc ttcaaattca gacagactga atgcgcttaa gggcaacgct ggcgagagga
     2761 atcgctgaag tccacgatgt ctctccagtt tgacgggtgc aggctgatct tgagcgtgta
     2821 gcgcgggttg gttctgcgtc tggtgggacg cacgatcacg cgatagtcgc cggtctgtct
     2881 ggcggagaag accacatcct gcgcgtcctc gacgcgctcg ccgtccggat ggatgaaaat
     2941 gaaatcggat gagccttctt cgtcatccgt cctgcgcctg atcgtgttgc cgtgatacag
     3001 aacgtccgtc acgatcacct gcccctgacg ggcgtgaaag atgtatgtgt ccctgcgtgg
     3061 cggttgcact gtgccgcgca aaatagtgca gggacgacgc gcgggcaggc ggactctacg
     3121 agacagcccg ccaccgtctt gtccctgtgc atttgccggg agcgtcaaca cacacatgaa
     3181 tgtgagtaat agagcgaagg cagcgagttt aaagttgata gttttccggt acatagaatt
     3241 tcagcagtca ggcaagcgtc tggaagttgt agacgcgcga gcttcgacag gcaaactcaa
     3301 tagcatgtga ttgtagcaaa ctcaaggaat tcaatttatg agcctgctat aaaatcctgc
     3361 gcagggcttt ggcaggtcgc ctgccgagga gcgcgagaga cgttaacagc agcagggtgg
     3421 cgagcgaaat ccacgcgccg gtgcgcgccg acggtgccgc atatttcatt tcgacgcgat
     3481 gcgcgccggg cgggacgtgc acgccgcgca ggagaaagtt ggtggtgtag attggccgtt
     3541 cgatgccgtc aaccgtggcc gtccaaccgg gatagttgat ctcgctaaag accaacaggg
     3601 cgtcagtctc tgcataggtg tctacggcca cgccgttcgg ttgataggag acatgcgccg
     3661 tggcgttcga cccgacaaga ccgccgggca gagcgggcag ttcgcccgtc gaaacttcga
     3721 gcagcgctgt gcgacgcgga tcgaactctt tgtcgctctc gccgcgtatg cgccgcaaag
     3781 cctcttcgcc atcgaccgct tcggcctcgg cgacgagcca cgcgcgcggc agggcacgct
     3841 cgtttcgcag gatcaggatg tcgtccgcgt cgtagacggt ctgccattta ccggggttga
     3901 gttcgacggg cagacgcgtc ccggcgtgag ccagcgtggt cgagcgtctg gtttccgagt
     3961 cgaagatggt ggttttccac aaggcgagcg atgcgcccgc tgcgacgttt tctatttcga
     4021 tctgctcgac ggcggcgcgt ctgccgagcg ggatgcttgc gcggtagcga tgcgcagtga
     4081 aagtgtcggg cgcgtcgccc ggcctgctat cgaaaacagg cgcgaggcta tggcggatgt
     4141 tgccgcgcac ctccgttcgc tcatgcgctc cgtcggctgt gtctgttccg gcgcgcaact
     4201 cgcgttcgag cgtctgcccg tccgaggtca ggatgcggag ctttgctacc acctcgccat
     4261 ccgcattgtt cgacgagtta gccagcgatg aaacgattgc cagcgtgtcg ccctgcgcag
     4321 acctgccgtg aataagagcc ttgcgccccg cctcgatgtg ccttccacgc tcgacgccgg
     4381 agtagctgat tcccgcctgt tcgagacgcg tgatcggagc ggcggagaag tctgagaagg
     4441 agacgaaaaa tttcgtgttc agcagatcga ggacatgaga gttgtttccg aagatagaga
     4501 ggtcggatgg aaaaccgggg cgcgggttga agccgtccat cccgacgttg ccgagcgcgc
     4561 ggctgtagcg ttcgaagatg agcggctcgt aacccgcgac gttatggatg cccgcgagca
     4621 tactcagatt gggcgcgtcc acgcgcgggc gttcggaaaa ctggtctgca aagagatcga
     4681 cgcgcaggta ggcgcggttc tgcaccggat catgatttcg caggaagcca cctgcgacgg
     4741 acggcggggc gaactggtct gccggtctag cccacggcag ccaccacaca gagagcagta
     4801 tgaacggctc catgaagcag gttaacaaga ccgctccgag aagctgtccc gtgcgccagc
     4861 gacctttcct gatctgccag cccgtccaaa tgctaacaaa ggtcaggatg gtaaaagcca
     4921 ccttccaccc ggcatatgaa ctctggacca gtcccttgtt caaagcatga ttcgggaacg
     4981 gagcaggggc taactgcgcc caccagatca ggccggagag tgccgccagc atcagtgtga
     5041 tcaaacttat gttgaacttg aggcgcgggc tttgcacagt cctgcgcgca tgccacgcag
     5101 ccagagcgtc ccagccgtag gctgacaaga ccgagacggc gaaagaccat tcgaacatgt
     5161 gacgcgccgg gcgtcggaac aggttgacga gtgggacgtt gtagagcaga cgattcagcg
     5221 gcgtgtagtc gccgagcatg agcgcccacg ccgcgagcgc agtcgccagc cagaagatga
     5281 cgcgcgcatc gcgcccgcgc cgcatcgcga agacgcctcc cgccccggcc aacacgaccg
     5341 ccagcagcgg catataggtt gttacttcca actcgtaata ggccgggaac agcagcgact
     5401 tcaagatcgt ctgcggggtg aaaaagccgc tgttgaaaag ctcgaagctg agcacgctgc
     5461 gaatgctgaa ccgcaccgcg cgcattgatt ccagcacttg aaaagcggcg actcccgcgc
     5521 ccgctacgac ggcgcagatc gccgcgaaca gtggccgcca gcgcgcggcg gagaaccacc
     5581 gggggcgttc gctgtcggcg tgcggcaggc gtccgcgcgg cggcgcgaga cagagaaagc
     5641 acgcataggc gacggcgatg atggcgacat acaaaaagcc ctgcccgtag ccgttcagca
     5701 cggacatggc ataagccaac ccgccccaga gcacgcaggt ggcgagtgtc cgcgttcgcg
     5761 cacgctcgat gaagaggagc agcaggggca gccacatcac cgcgttcgag gtcaggccgt
     5821 tatggccgag aacgttggtc gtcaggccgc tgtaggcgaa gcccagcccg gccagtagtg
     5881 ctgcgatgcg gctcaatccc agttcacgaa tgtaggcgta gaggaaggcg ggggcgaaga
     5941 gaaaagggga gagcacgtaa acttgttccg cccacacgcc gggcaggaac aggtagcccc
     6001 acgtgaacgg gtagccgagg gagagttgcc ccatcgagag cagcgggtag cccgaaaata
     6061 tgagggagg
//
LOCUS       DASWNS010000276        15073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_165618, whole genome shotgun sequence.
ACCESSION   DASWNS010000276 DASWNS010000000
VERSION     DASWNS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15073
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165618"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1050)
                     /locus_tag="VGB76_09785"
     CDS             complement(148..1050)
                     /locus_tag="VGB76_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEY0099233.1"
                     /translation="MIRLLALDLDGTLLNSLGELSERNALAIAGARAAGVRVALVTGR
                     RFRDARPLALKLGLDVPVVAHNGALTKHARTLETVNASLLPLATAREVLRLGRAYRAD
                     PLVSDDAEGAGVLVYDHLRDTAGPVAEYIEWSRRIHGDEAERAILHVPSLEEYLDHAP
                     VHITFSGTFSDIERLSGVFARELGASAKLMTTLYPSIDFGLIDVIHPDVSKGTGLAAV
                     ASEHNLTPAEVMAVGDNHNDVEMLEFAGTRVAVSNAEPRVLELAGIHVTESNDEDGVA
                     AAIEKLILAERSNGNSEFRIQHSE"
     gene            1322..2485
                     /locus_tag="VGB76_09790"
     CDS             1322..2485
                     /locus_tag="VGB76_09790"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY0099234.1"
                     /translation="MNVTHLECAACGLRHEARQLLNLCRECGKPLLVRYDLERAAAQL
                     KKESLRERAGDLWRYREVLPVAHVENIVSLGEGWTPLLRAARLGAKLGLSELYIKDES
                     QNPTQSFKARGMTAAVSMAKELGARKLAVPSAGNAAGALAAYAARAGLEAHIFMPRDT
                     PRANIVECEQMGAHVTLLDGLITDCGAEVGRRKEAEGWFDVSTLKEPYRVEGKKTLGY
                     ELAEQFGWELPDVIIYPTGGGTGLIGMWKAFEEMEAMNWIGSKRPRMVTVQAAGCAPI
                     VRAFAEGKRFADEFPDAATTASGLRVPKAIGDFLILDALRASNGTAISVTDAELIAAT
                     REVGAAEGLFCAPEGAACLPALEKLLAAGLVKPEERIVLFNTGAGVKYLESFS"
     gene            2521..2856
                     /locus_tag="VGB76_09795"
     CDS             2521..2856
                     /locus_tag="VGB76_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099235.1"
                     /translation="MARLIFFASALNVAGILCLNAATRAPAAEPIVGQEFGYAGWSRG
                     WASLLDSFGFTLMMPGIFFASSVFLGAHVLALNERAARSIWYATGFGINLLTVWKVGV
                     VCEAVRERV"
     gene            2858..4234
                     /locus_tag="VGB76_09800"
     CDS             2858..4234
                     /locus_tag="VGB76_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099236.1"
                     /translation="MIGEVAMKRRLPAVLFVLHVTVLLLIILLASYSLAHAQGAATKL
                     QPGGANKSPAANTSTMAEVERARFDRARTEGFEALYNLDYETARARFKELARLYPEHP
                     AGPQFLAASLWLETLNASRRLQSGLYNNDSFYAKTEDKVDPKIVAQFKEWTRTAKSLA
                     EARLKKNPQDTEALYFLGATQGLKAAFAGAVERSFVSALRSGSDAVDKHRETLKLDPN
                     YHDAALSIGMQDYIVGGLPLPYKILAGMTGTRGSKKRGLATLERVMREGQWARDDARV
                     MLIPLYKREKRFADAAALARELAAKYPKNYLFKLEYADALVSQAATERAGDGGGQAAT
                     ATEREAFAIFDSLLRDKATRDAAARQLDLIHFQYGEALFVAGQTERAAKEFLAAASAP
                     NAEANLATLARLRAAQSFDLAGRREAALAEYKLVLARPNVYDSLEEARRGMREPYRKV
                     AAKPQEDN"
     gene            4399..5676
                     /locus_tag="VGB76_09805"
     CDS             4399..5676
                     /locus_tag="VGB76_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0099237.1"
                     /translation="MQKNLNGGAGQTRPRRLLLCLDGVPYEVIKTAKSRGLFARFEKP
                     AGLLSPFPTMTNIALSTMLGATPPNGYESLYFDCDAQELRGGIRKYIGRRTPDKVPSS
                     YMDELDYQEPLPFEFLVYVAPDAVWRADMRRFRDKFQNAPQDRDFFAFLKGTDGLLHI
                     RGPQKLNVALESLDRILKEIQDVCGAETEIVLFSDHGMNLEENQRIHLQTHLRANGYD
                     VKDRFERGRGRGKVAAPAFGLCGYAAIYCADETDPAQVAGALTTLEGVDFSIHREGES
                     AVVVHGARGTARISREANGSARYSYEQLTGDPLQLAEAQRTLRERGELDARGYAADAA
                     WLAETAGHIYPDALANLYASLHAPRVRHTADVLVSCHDGYYYGMSFFSRFVRLAATHG
                     NALRASSTAFLMSTHREFPTHVRAADARPFLRD"
     gene            5977..6585
                     /locus_tag="VGB76_09810"
     CDS             5977..6585
                     /locus_tag="VGB76_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099238.1"
                     /translation="MGVKAQSKASAFLGAWVLDKEKTSAKDFPKKLQNYRMVVAESEN
                     LLNVKNQVEGRVEVEISRDRAADVGPNITPNTVGAPPGQRAGGLTPGAPKSNYSGTLA
                     LSFTPGQFVYDLSGKEVKVEIKRGEKVNGYARITAKLDKNGKTLKLTTLRREKMPYGE
                     LETNVWETWKLSEDGQSLKFTRTVETPTVRDELTLMLSRASQ"
     gene            complement(6672..8843)
                     /locus_tag="VGB76_09815"
     CDS             complement(6672..8843)
                     /locus_tag="VGB76_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0099239.1"
                     /translation="MKLRFALSLSCLLLWYVAATSFAQTPSPSVPLPAGERDDVVVIT
                     TNLIQIDATVTDKDGRAVPDLRQEDFEIYQNGKLQPITNFSFIALQPEDRNRLARDGK
                     ESDGAPPLASSRLRYQQVRRTIALVVDDLGLSFASTNWTKQALRKFVDEQLQPGDLVA
                     IIRTSSGVGTLQQFTTDRRLLHAAIERLRWNVRGGGGSFSPIEANLSEAMSNRVIVNA
                     EGERELHMVGGMKEEREFQRDAFESQESVFAAGTLGAVNFVMRGMGQLPGRKAVILFS
                     DGFKLFSRKGQNIRVQDALQKLTDTANRSGVIIYTMDPRGLDAPMLSAQDSTYGLTGD
                     QVDALVEERRQDYFDTQQGLRAIASRTGGFSIINQNNLTKGIERILDDQKGYYLIGYK
                     PDADVFDIEKRRFNSLTVKVKRPGLRVRYRSGFFGMLDAEARPQPRNAVQQLIAALTS
                     PFTSKDIDLRLTSLFAGDTTANAFMRSLIYIKAGDLTFKEEKDGWHQAEFEVSVLTFS
                     DNGEVVDQINRKQTVRARGAYLKNIYADGLVCSVTLPIQKPGAYQMRVAIRDTATERI
                     GSASQYIEVPNLKKKNLTLSGIVLERIEAKNSNSVADAKTLELKMQRDAAMRRFRLGE
                     TIRFGFSVYNAKFDKASGQPTLSMQYKVFQNGKQIFASQEKPLQNAPLQELKVVDISD
                     AFSLGRKMEQGDYVVQVVVRDALAKGSAQTATQWTDFEIVP"
     gene            9099..9860
                     /locus_tag="VGB76_09820"
     CDS             9099..9860
                     /locus_tag="VGB76_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099240.1"
                     /translation="MAKDISKTQIDRLGERLKKGNITEADLRLLDLYRRSFTPAYEIV
                     VRTIRNDLALEPTGRPAKSTTSISEKLRRESIRLTQIQDIAGCRLTVTDIVEQDSVVE
                     SLRNLFDKTTIVDRRQQPSHGYRAVHVVVSCLNKMIEIQVRTSLQHLWAELSEKLSDM
                     VNPAIKYGAGDERVRWILANASNMVVRTESLEVDWVNLKGNLSPSDMFTEDIQQIIAE
                     NEKILTSLRQQVFKGLHDVIEYLEEPAGENDDLSD"
     gene            9844..10083
                     /locus_tag="VGB76_09825"
     CDS             9844..10083
                     /locus_tag="VGB76_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099241.1"
                     /translation="MIFLIEYDRARGEIVTFQTFNDLERQNAENARLEMELQLNRLGT
                     EREIVLLEAATEEALRRTHRRYFEDLAELVNSPAR"
     gene            complement(10154..11428)
                     /locus_tag="VGB76_09830"
     CDS             complement(10154..11428)
                     /locus_tag="VGB76_09830"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0099242.1"
                     /translation="MFIDLTPLRISRDYRLLFFGQLISAFGTAMSFVVVPVQVYQLTG
                     STLLVGLLSASEFVLILLLAFVGGAYADFIDKRKMLRLTEIGQTLVTTALVFNATLAR
                     PQVWLLFVCAALHAGLAALQRPSFEALMQKIVTPDLMPSISALNSLRWNLTTILGPSL
                     AGIILARFGAEVAYSIDLVTFIASLTAVFLIRATPTPKQLEERPTLKSVVEGVRYAWR
                     RKEILGTYLIDMNAMFFGMPTALFPAVAANFGAETVGLFYAAPSVGALLATLTSGWAK
                     HVHRHGLFVSLAAALWGVAMIFFGFADNLYLALFCLALAGGFDMISGVFRMTIWSQTI
                     PHHLRGRLAGLEMISYTSGPKLGDAEAGLVAALFSVRASVVSGGILCIVGTIIISALL
                     PQFLRYDGREGVKQKEIEDALRETENRTLILE"
     gene            11666..12496
                     /locus_tag="VGB76_09835"
     CDS             11666..12496
                     /locus_tag="VGB76_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0099243.1"
                     /translation="MNNQLETERVPAVETFELVRRFGDFTAVDHVNLSVERGQFFGFL
                     GPNGAGKSTTIKMLTGLLQPTQGRVRVLGRDLAVETLEVKRRIGVVPEDLNLFERLTG
                     AEMLTFTGRMYGLTREETTERGRELLALMELDAEPKKLVVEYSHGMKKKLSLACALIH
                     RPEILFLDEPFEGVDALASRTLKDLLLKLTARGITVFLTSHVLEIVERLCTHIAIITE
                     GRLVVEGALDELRRGIALEDESVAGSSGGGSPLSLEDYFIRVIGGARPAGEEVLTWLA
                     "
     gene            complement(12646..14817)
                     /locus_tag="VGB76_09840"
     CDS             complement(12646..14817)
                     /locus_tag="VGB76_09840"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0099244.1"
                     /translation="MRSLVQFSRARLTAQLLGFALCLFCYGAQSGASAQAVNQYRFTS
                     TNGAAVVPGNTPVTNFACGSVPGDDCVATVNLPFAYTFYTNSYTQANVTSNGTLQFVT
                     ANTDYGLNELCLPLLHFSYSILPHFTDLTIGGAGEGVFTSVSGTAPNRIFNIEWRASQ
                     FGKPAGSLNFEVRLYEGQTRFDIIYGTVPGNGLEATVGVQRVAAAGDPAGAFTQYECH
                     TGGLRNGQMLVAQGVQSNSLFIEGQVVDMEGNPLPGVTINLTGAGTATTTTDATGHYL
                     IEGLVAGSTHTITAVEGNSTFYPASRTFASYQGNQTVNFTRTVLPEAGQLLISEFRLR
                     GPFPIGGDPATGGARDEFIEIYNNTNSTFVVNTADGSNGWAITSSNTLAPAVRYVIQR
                     GTIIPPRGHFLVGNGSGYSLTAYASPDAFYGGDIPDNSGLALVRTATAANYELPGNIL
                     DAAGFEAETNVAYREGTGLPSMPTDNAQYSFVRKLTSGTPQDTGDNAQDFVLVSTSAA
                     MFGGIQSILGAPGPQNSGSHLQRNATIKSSLIDTQCAGFGSPASACARVRTAQNANAQ
                     NAAFGTLLLRRKFTNVTGQKINRLRFRVVDLSTKGNEQANQADLRAISSSDVTVTDTN
                     NQSVFLRGLTLGQSFDQPEGGGINSTLSVNSITLSTPLAPGASINVEFRLGVMTNGNF
                     RFFANVEAVTAPVPPPAPEDGIAAPSVNTKRTAGKARRSAR"
BASE COUNT         3075 a         4288 c         4789 g         2921 t
ORIGIN      
        1 cttgatcttc caataactcg aagcggatcg tgccgtggtt cgtcttgatg tttgcgatgc
       61 gatttgccat tagtctctcc tcaatgaaat gtcgaatcgg aatatggaat tcagaataca
      121 ggagtcagga gacagccgga cacgctttta ttctgagtgc tgaattctga attctgaatt
      181 cccgtttgat ctctccgcca ggataagttt ctcgattgcc gctgcgacgc cgtcttcgtc
      241 gttgctctcc gtgacgtgga tgcctgcgag ttcgagcacg cgcggctcgg cgttcgagac
      301 ggcgacgcgc gttccggcaa actccaacat ctccacatcg ttgtgattgt cgccgacagc
      361 catcacttcg gcgggcgtca aattatgctc cgaggcgacc gccgccagcc ccgtgccctt
      421 cgacacgtcg gggtgaatca cgtcaatcag cccgaaatca atcgaaggat aaagcgtcgt
      481 catcaacttc gcgctcgcgc cgagttcgcg tgcgaagaca ccgctcaggc gttcgatgtc
      541 ggagaacgtg ccggagaagg tgatgtggac gggcgcgtgg tcgagatact cttcgagcga
      601 ggggacgtgg aggatggcgc gctcggcttc gtcgccgtga atgcggcgcg accattcgat
      661 gtattcggcg acaggccccg ccgtgtcgcg caggtgatca tagacgagca cgcccgcgcc
      721 ctccgcgtcg tcgctcacga gcgggtcagc gcgataggcg cgcccgaggc gcaggacttc
      781 gcgcgccgtc gccagcggca acagactcgc gttgacggtt tcgagcgtgc gcgcgtgctt
      841 ggtgagcgcg ccgttatgcg cgacgacggg cacgtctaaa ccgagtttga gcgcgagcgg
      901 gcgcgcatcg cgaaaacgac gccccgtgac gagcgcgacg cgcacccctg ccgcgcgcgc
      961 tccggcgatg gcgagcgcgt tacgctccga caactcgccg agcgagttga gcagcgttcc
     1021 gtcgaggtcg agcgcgagca ggcgaatcat aaaaatagtg acgagtgaca agtgacgagt
     1081 gacaagatta aaccagtgaa tcgtaaatcg taaatcgtca acagcgaaaa cggattttcg
     1141 tctgttgacg atttacgatt tactgttcaa ggcttttccc gctcatcact tgtcactcgt
     1201 cacctgtcac tgctttcctg ccactgtcgg gcatttgctc cacctgacgg atgtttgatg
     1261 gctgacgtat gatgcgggca ttgaacgaga aggaaaaaat tcggaaagga cggggttttc
     1321 tatgaatgtg acacacctgg aatgcgcggc gtgcgggctg agacacgagg cacggcagtt
     1381 gctaaatctc tgtcgtgagt gcggcaagcc tctgctggtt cgctacgatc tggagcgcgc
     1441 cgccgcacag ttgaagaagg aaagtttacg agagagggct ggggatttat ggcgttaccg
     1501 agaggtgctg cccgtcgcgc acgtagaaaa tatcgtctcg ctcggcgaag gctggacgcc
     1561 gctgctgcgc gccgcacgat tgggcgcgaa gttgggcttg agcgaacttt acatcaagga
     1621 cgaatcgcag aatccgacgc agagcttcaa ggcgcggggg atgacggcag ccgtctcgat
     1681 ggcgaaggag ttgggcgcgc gtaaactcgc cgtgccctct gcgggcaacg cggcgggcgc
     1741 gctcgccgcc tacgccgcac gcgccggcct tgaggcgcac atcttcatgc cgcgcgacac
     1801 gccccgcgcc aacatcgtcg aatgcgagca gatgggcgcg cacgtcacgc tgctggacgg
     1861 actcatcacg gactgcggcg cggaagtcgg gcgacgcaaa gaggcggaag gctggttcga
     1921 cgtttcgact ttgaaagaac cgtatcgcgt cgaaggcaaa aagacgctcg gctacgagtt
     1981 ggcggaacag ttcggatggg agttgcccga cgtgatcatc tacccgacgg gcggcggcac
     2041 cggactcatc ggcatgtgga aggcatttga agagatggaa gcgatgaact ggatcggcag
     2101 caaacgcccg cgcatggtga cggtgcaggc cgccgggtgc gctcccatcg tgcgcgcgtt
     2161 cgccgaaggc aagcgtttcg cggacgagtt tccagacgcc gccacgaccg cttcgggctt
     2221 gcgcgtgccg aaggccatcg gcgacttttt gattctcgac gcgctgcggg cttcaaacgg
     2281 cacggccatc agcgtgacgg acgcggaatt gatcgcggcg acgcgcgagg tcggcgcggc
     2341 ggagggtctg ttttgtgcgc cggaaggcgc ggcgtgcctg cccgctctgg aaaagttgct
     2401 ggcggcgggg ttggtcaagc cggaggagcg cattgtgctc ttcaacacgg gcgcgggcgt
     2461 gaaatatctg gagagttttt catagtgaaa cggcgcacac agaagcaaag ccgggcgttt
     2521 ttggcgcgtc taatattctt cgcgtcggcc ttgaacgtgg cgggcatttt atgtctgaac
     2581 gcggcgacgc gcgccccggc ggcggagcca atcgtggggc aggagttcgg atacgcaggc
     2641 tggtcgcgcg ggtgggcgtc gctgctcgat tcgttcggat tcaccttgat gatgccggga
     2701 atattcttcg cctcgtcggt ctttctgggc gcgcacgtct tggccttgaa tgaacgtgcg
     2761 gcgcgttcga tctggtacgc gacggggttc gggatcaatc tgttgacggt atggaaagtc
     2821 ggcgttgtgt gcgaagcggt gcgggagaga gtttgagatg attggagaag ttgcgatgaa
     2881 acggcgtttg ccagcggtgc tgtttgtgct gcacgttacg gtgctgctgc tcataatttt
     2941 gttggcctct tacagcctcg cgcacgcaca gggcgcggcg acgaagttgc agcccggcgg
     3001 agcgaacaag agtccggcgg caaacacttc gacgatggcc gaagtcgagc gtgcgcgctt
     3061 tgatcgtgcg cgcacggagg gcttcgaggc tctctacaat ctcgactacg aaacggcgcg
     3121 ggcgcgtttc aaagaactcg cgcgcctcta cccggagcat ccggcgggac cccagttcct
     3181 ggccgcgagc ctgtggcttg agactctgaa cgcgtcgcgc cgtttgcagt cgggtctgta
     3241 taacaacgac tctttctacg caaagacgga agacaaggtt gacccgaaga tcgtcgcgca
     3301 gttcaaagaa tggacgcgca cggcgaagag tctggcggaa gcgcgcctga agaagaaccc
     3361 gcaggacacg gaagccctct attttctcgg cgcgacgcag ggcttaaagg ccgcgtttgc
     3421 gggggcggtc gagcgtagtt tcgtctcggc tctgcgcagc gggtcggacg cggtggataa
     3481 acatcgtgag acgctcaaac tcgatccgaa ctatcacgat gcggcgttga gcatcggcat
     3541 gcaggactac atcgtcggcg gcctccccct gccttacaaa attctcgccg ggatgaccgg
     3601 cacgcgcggc tcgaagaagc gtggcctggc gacgctcgaa cgcgtgatgc gcgaagggca
     3661 gtgggcgcga gacgacgcgc gcgtgatgct catcccgctc tacaaacgcg aaaagcgatt
     3721 cgcggacgcc gccgcgctgg cgcgcgaact cgccgcgaag tatccgaaga attacctgtt
     3781 caagttggag tacgcggacg cgctcgtctc gcaggcggcg accgagcgcg caggcgacgg
     3841 cggcgggcag gccgcgacgg cgaccgagcg cgaagccttc gccatcttcg acagcctcct
     3901 gcgcgacaag gcgacgcgcg atgcggctgc gcgccaactc gatctcattc atttccaata
     3961 cggcgaagct ctcttcgtcg cggggcagac ggagcgcgcg gcgaaagagt ttctggcggc
     4021 ggcgtctgcg ccgaatgcgg aggcgaactt agcgacgctc gcgcgcctgc gcgccgcgca
     4081 gtccttcgac ctcgcgggca gacgcgaggc cgcgctcgcc gagtacaaac tcgtgctcgc
     4141 gcgcccgaac gtctacgact cgctcgaaga agcgcggcgc ggcatgcgcg aaccctatcg
     4201 caaagtcgcg gcgaagccgc aggaagataa ctaaaagtgt tcgttgtccc gtggtcgccc
     4261 gccagttgtt tgagttcata tgtcacctgc taccacagtc tgcattgaac caacggcgga
     4321 cggccacggg caacgggcaa cggacaaaaa tcaacggaca acgaacaacc ggctactgac
     4381 aaggatacag gtgaagtggt gcaaaagaat ttaaacggcg gggcggggca gacgcgaccg
     4441 cgccgcctgc tgttgtgtct cgacggcgtg ccttacgagg tgatcaaaac ggcgaaatcg
     4501 cgcgggctgt tcgcgcgctt cgagaagccc gccgggttgc tctcgccctt cccgacgatg
     4561 acgaacatcg cgctctcgac gatgctcggc gcgactccgc cgaacggcta cgagagcctc
     4621 tatttcgatt gcgacgcgca ggagcttcgc ggcggcatac gcaagtacat cggtcggcgc
     4681 acgcccgaca aagtgccttc gtcctacatg gacgaactcg attatcaaga gcctctgccc
     4741 ttcgagtttc tcgtctacgt tgcgcccgac gcggtgtggc gcgcggacat gcgccgcttc
     4801 cgcgacaagt ttcagaacgc gccgcaggat agagacttct tcgccttcct caaaggcacg
     4861 gatggcctgt tgcacatacg cggaccacag aagttgaacg tcgcgctcga atcgctcgac
     4921 cggattctca aggagattca ggacgtgtgc ggcgcggaga cggagatcgt cctcttctca
     4981 gaccacggca tgaacctcga agagaatcag agaatacatc tccagacgca cctgcgcgcc
     5041 aacggctacg acgtgaaaga ccgcttcgag cgcgggcgcg gcagaggcaa agtggccgcg
     5101 cccgccttcg gcctctgcgg ctacgccgcc atctattgcg ccgacgagac agaccccgcg
     5161 caggtcgccg gtgctttgac gacgctcgaa ggagttgatt tcagcatcca tcgcgaaggc
     5221 gagtcagccg tcgtcgtaca cggtgcgcgc ggcacggcgc gcatcagccg cgaagcgaac
     5281 ggctctgcgc gttacagcta cgaacaactc acgggcgatc ctctgcaact cgcggaagcg
     5341 cagcggacgc tacgcgaacg cggcgaactc gacgcgcgcg gctacgctgc cgacgccgcc
     5401 tggctggccg agactgccgg gcacatctac ccggacgcgc tcgccaacct ctacgcctcg
     5461 ctccacgccc cgcgcgtgcg ccacacggcg gacgtgctcg tcagttgcca cgacggctac
     5521 tactacggca tgtctttctt cagccgcttc gtgcggctcg ccgccaccca cggcaacgcc
     5581 ctgcgcgcga gctcgaccgc cttcctcatg agcacgcacc gcgaatttcc cacgcacgtc
     5641 cgcgccgcag atgcgcgccc tttcctgcgc gattagccgg ggagttataa gtcccaagtc
     5701 tcaagtcccc agtcccgcag ccgcgtgggc tgtgggactt gagactttcg ctcccggttc
     5761 ccgagggtct tctacaaaag aattgatcaa agccgcgcgg ggtgtgtaga atgcaccgga
     5821 aaaacgcgtg gccttccggc atctgcgaca acttagagag acaaaaaaag ctcccctgaa
     5881 aatattctcc ggctctcgct ggttgaaaga aagagatcaa tcatgaaaaa actatgcttc
     5941 agcgtaatct tcgcactcct tttcttaacg ccgtccgtgg gcgtcaaagc acagtcgaag
     6001 gcatcggcgt tcttaggggc ttgggtgctg gataaggaaa aaaccagtgc caaggatttt
     6061 cccaaaaaat tgcagaacta caggatggtc gtcgccgaaa gcgagaacct cctcaacgtc
     6121 aaaaatcagg ttgaaggccg ggtggaagtg gaaatttcga gggatcgcgc ggcggacgtg
     6181 gggccgaata tcacccccaa cacggtcggc gcacctcccg gccagagggc tggcggcctg
     6241 acgccgggcg caccgaagag caactatagc gggacgctgg ccttgtcctt tacgccgggt
     6301 caattcgttt atgatctcag cggcaaggaa gtgaaagtcg agatcaagcg cggcgagaag
     6361 gttaacggct acgccagaat tactgcgaag ctggacaaga acggcaagac gctgaaactc
     6421 acgaccttgc gccgcgagaa gatgccgtat ggcgaattgg aaaccaatgt ctgggagact
     6481 tggaaacttt cggaagatgg tcagtcgctt aaattcacgc gcaccgtcga gacgccgacc
     6541 gtgagagatg aactcacgct gatgctgagc agagcctccc agtaaggata accgctcgct
     6601 agcctcccgg cggccaacgt tccgcctccg gcgcgttggc cgcctgcttt ctctccgcct
     6661 gccttctctc ctcaggggac gatctcgaaa tcagtccact gcgtcgccgt ctgcgcgctg
     6721 cctttggcga gcgcgtcgcg gacgacgacc tgcacgacat aatctccctg ctccatcttc
     6781 cttcccagag agaacgcgtc cgagatgtct acgactttaa gctcctgtag cggtgcgttc
     6841 tgcaagggct tttcctgcga agcgaagatt tgctttccgt tctggaaaac tttgtactgc
     6901 atggaaaggg tcggctgccc gctcgccttg tcgaacttcg cattgtagac ggagaacccg
     6961 aacctgatcg tctcgcccag acggaaacgg cgcatggcgg catcgcgctg catcttgagt
     7021 tcgagggttt tggcatcggc gacggagttt gagtttttcg cttcgatgcg ttccagcacg
     7081 atgccggata gcgtcaggtt cttcttcttg aggttgggca cttcgatgta ctggctcgcc
     7141 gatccgatcc gttcggtcgc cgtgtctctg atggcgacgc gcatctggta tgcccccggc
     7201 ttctgaatcg gcaaagtgac ggagcagacc agcccgtccg cgtaaatgtt tttgagatac
     7261 gccccgcgcg cacgcaccgt ctgtttacga ttgatttgat caaccacctc gccgttatcg
     7321 ctgaaggtca gcaccgacac ttcaaactcg gcctgatgcc aaccgtcctt ctcctccttg
     7381 aaagtcaaat ctccggcttt gatgtagatc aacgaacgca tgaaagcgtt ggcggtcgtg
     7441 tcgcccgcga acagagaggt gaggcgcaaa tctatatcct tcgaagtgaa gggcgaggtc
     7501 agcgcggcga tcaactgctg caccgcgttt ctcggttgcg gcctcgcttc ggcgtcgagc
     7561 atgccgaaga aaccgctccg gtaacggacg cgcagcccgg ggcgtttgac cttgaccgtg
     7621 agggaattga agcggcgttt ctcgatgtcg aaaacatctg cgtccggctt gtagccgatg
     7681 aggtagtagc ccttctgatc gtcgagaatc cgctcgatgc ctttggtcag attattctgg
     7741 ttgatgatgg agaaaccgcc cgtccggctg gcgatggcgc gcaagccctg ctgcgtgtcg
     7801 aagtagtcct gccttctctc ttccaccagc gcatccacct gatcgcccgt cagcccgtaa
     7861 gtgctgtcct gcgccgagag catcggggcg tcgaggccgc gcgggtccat cgtgtagatg
     7921 atgactcccg aacgattcgc cgtgtccgtc agcttttgca gcgcgtcctg cacgcgtatg
     7981 ttctggcctt tccgggaaaa aagtttgaag ccgtcggaga agaggatgac ggccttgcgc
     8041 cccggaagct gacccatccc gcgcatgacg aagttgaccg cgccgagcgt cccggcggcg
     8101 aagacgctct cctgcgactc aaacgcgtcg cgctggaatt cgcgctcctc tttcatcccc
     8161 ccgaccatgt ggagttctct ctctccttcc gcattcacaa tcaccctgtt gctcatcgct
     8221 tcgctcaggt tggcttcaat cggggagaac gatccgccgc ctccgcgcac gttccagcgc
     8281 aggcgttcaa tcgcggcatg gagcaaacgc cggtcggtcg tgaactgttg cagcgtgccg
     8341 acgccgctgc tcgtgcggat gatggcgacc agatcgccgg gttgtaattg ctcatccaca
     8401 aacttgcgta gagcctgttt cgtccaattg gtgctggcaa acgaaagccc cagatcgtca
     8461 accacgagcg cgatggtgcg gcgcacctgc tggtagcgga gacggcttga ggcgagcgga
     8521 ggcgcgccat cggattcttt cccgtcacgc gcgagcctgt ttctgtcctc cggttgcagg
     8581 gcgataaagg agaagttggt aatcggctgc aacttgccgt tctggtagat ctcgaaatcc
     8641 tcttggcgca aatccggcac ggcgcgcccg tccttatccg tcacggtggc gtcaatctgg
     8701 atcaggttcg tggtgatgac gacgacatca tctctctctc cggctggcag cggcacggac
     8761 ggcgaagggg tttgtgcgaa tgacgtagcg gcgacgtacc acagcagcag gcacgacaac
     8821 gacaaggcga agcgtaattt catatagcat ccttaaagcg ttcgggaatg gattaaatcg
     8881 tcggccttcc gtcggcgatt caaactcagg gcagcacagg caaactacgc ctcccgcccc
     8941 gattcgtcaa tagtcacgcg ccgacagtcg cccgcccacc cccgcccacc cccgcacgcc
     9001 cattccctcc gtaatcagtt gcgagagaaa taggccgtct gcaagagact ggatggcgca
     9061 aagccgaagg tgctaagatg ccgcgaaata aggcatctat ggcaaaggac ataagcaaga
     9121 ctcagatcga ccgcttaggc gagcggttga agaagggcaa tattaccgaa gcagatttgc
     9181 ggcttctcga cctgtaccgc cgttcgttta ctccggccta cgagattgtc gtaagaacta
     9241 ttcgtaacga tctggcgctt gagccaacag gcagacccgc caaatcaacc acttccatct
     9301 ccgagaaact gcgccgggaa agcatccgcc taacccagat tcaagacatc gcgggctgta
     9361 ggctgacggt cacagacatc gtcgagcaag actcagttgt tgaatctctc cgcaatctct
     9421 ttgacaagac caccatcgtt gaccgtcgcc agcaacccag ccacggctat cgcgccgtgc
     9481 atgtcgtcgt gagttgtctc aacaaaatga tcgagataca ggtgagaact tcactgcaac
     9541 atctctgggc tgagctatca gaaaaattat cggatatggt taatcctgcg attaagtacg
     9601 gagcgggtga tgaaagggtg cgctggatcc tagcaaatgc atccaatatg gttgtccgca
     9661 cagaatcatt ggaggtagac tgggtaaatc taaaggggaa cttatctccg tcagatatgt
     9721 tcacagaaga tatacagcaa ataatagccg aaaatgaaaa gatactaacc tcactgaggc
     9781 aacaggtatt caagggtctt catgatgtta ttgagtattt ggaagaaccg gcaggagaga
     9841 acgatgatct ttctgattga atatgatcgc gctcgtggag aaatagttac cttccagacg
     9901 ttcaacgact tggagcgtca aaatgctgaa aacgcgcgtc tcgaaatgga acttcaactc
     9961 aaccgtcttg gtactgagcg tgagattgtc ttattagaag ctgctaccga agaggctttg
    10021 cggcgtactc atcgtcgcta ctttgaggat ttagccgaac tcgtgaactc gcccgcgcgg
    10081 taaggataga ttgctcgccg ccaaatccat tccgcacgta aagcccgccc acttgtctga
    10141 aaaactctga gagttattcg aggattaagg ttctgttttc ggtttcgcgg agggcgtctt
    10201 cgatctcttt ttgtttgacg ccttcgcgcc cgtcgtagcg taggaattgg gggagcaggg
    10261 cggagatgat gattgtgccg acgatgcaga ggatgccgcc ggaaactacg gaggcgcgga
    10321 cgctgaaaag ggcggcgacg agtccggctt cggcgtcgcc gagttttggt cccgaggtgt
    10381 agctgatcat ctcaagcccg gcgagccgcc cgcgcaggtg gtgcgggatc gtctggctcc
    10441 agatcgtcat ccgaaaaact ccgctgatca tgtcgaagcc gcccgccaga gcgagacaga
    10501 acaaggctag gtataaattg tctgcgaacc cgaagaagat catcgccacg ccccagagcg
    10561 cggcggcaag gctgacgaac aggccgtgac gatgcacatg cttcgcccag cccgaagtca
    10621 aagtggcgag gagcgcgccg acggacgggg cggcgtagaa gaggccgacc gtctccgcgc
    10681 cgaagtttgc ggccacggcg ggaaagagcg cggtcggcat gccgaaaaac atcgcgttca
    10741 tatcgatcag gtaggtgccg agaatctcct tgcgccgcca ggcgtaacgc acgccttcga
    10801 cgacggattt cagcgtcggg cgttcttcaa gttgctttgg cgtgggcgtg gcgcgaatca
    10861 gaaagacagc ggtgagcgaa gcgatgaacg ttaccaaatc tatcgaataa gctacctccg
    10921 cgccgaaccg cgccaaaatt attccggcca gcgaagggcc taagattgtc gtcaggttcc
    10981 aacgcagcga attcaacgcg ctgatggaag gcatcaaatc cggcgtcacg atcttttgca
    11041 tcaacgcttc gaacgaagga cgctgcaagg cggccagccc ggcgtggagc gcggcgcaga
    11101 cgaataacaa ccagacctgc gggcgcgcga gcgtcgcatt gaagacgagc gcagtcgtca
    11161 cgagcgtctg cccgatctcc gtcagccgca gcatctttcg cttgtcgatg aaatccgcat
    11221 acgcgccgcc gacgaacgcc agcaacagga tcaagacaaa ctccgacgcg ctcagtaagc
    11281 cgaccagaag cgtcgagccg gtcaattgat agacctgcac gggcacgacg acgaaactca
    11341 tcgccgtgcc gaaagccgag atcagttgac cgaagaagag caggcgataa tcgcgggaga
    11401 tacgcagggg cgtcaggtct ataaacatta aagcggcaga agaagaggtt tgaattgagc
    11461 acacgaacga ggcgtcgcta ctgttgacgc agacgcaaaa ttgcttcgcc ccggacgcgg
    11521 aatcatacga cgaatgcgcg cggcgtccat aaccgtcaac acactttgtc ggtcgtgtga
    11581 gcggtcgcgt caggaggtga gcctgcccgt tccgtgcgcg cgttctgctt gaccgtgcgc
    11641 ctgctctgcg cgtacaaaac agtggatgaa taatcagctt gagacagagc gcgtgcccgc
    11701 cgtcgagacg tttgaactcg tgcgacggtt cggggatttc actgccgtcg accacgtgaa
    11761 cttgagcgtg gagcgcgggc agttcttcgg ctttctgggt ccgaacgggg cggggaagtc
    11821 aacgacgatc aagatgttga cggggctgct gcaaccaacc caggggcgcg tgcgcgtgct
    11881 agggcgcgat ctggccgtcg agactctgga ggtgaaacgg cggatcggag ttgtgccgga
    11941 ggacttgaat ctgttcgagc gtctgacggg cgcggagatg ctgacgttta cggggcggat
    12001 gtacggcctg acgcgcgaag agacgacgga gcgcgggcgc gagttgctcg cgttgatgga
    12061 gttggacgcg gagccgaaga agctggtggt cgagtattca cacgggatga agaagaaatt
    12121 gtcgctggcg tgcgcgctga ttcacaggcc ggagattctg tttctggacg agccgttcga
    12181 gggcgtggat gcgctcgctt cgcgcacgct caaggatttg ctgttgaagt tgacggcgcg
    12241 cggcatcacg gtctttctca cgtcgcacgt gttggagatc gtcgaacgcc tctgcacgca
    12301 catcgcgatc atcacggagg ggcgtctggt ggtggagggc gcgctcgacg aactgcggcg
    12361 cggcatcgcg ctcgaagacg aaagcgttgc ggggagtagc ggcggcggca gcccgctctc
    12421 gctcgaagat tatttcatac gcgtgatcgg cggggcgcgt ccggcgggcg aagaggtatt
    12481 gacgtggctg gcgtgagaag aagggatgag gggtgaagtt ttagaaccgg agttgaagcg
    12541 acttttgttt gctgtttaag tcgggcgcgg gagattgtga gaggcacggg cgacgagaag
    12601 attgattctc gtcgcccgtg cctttcgcgc gttccggggt tggtcttagc gtgcggagcg
    12661 gcgcgctttg cccgccgtgc gtttggtgtt gacggagggc gcggcgatgc cgtcttcggg
    12721 cgcgggcgga gggacgggcg cggtgaccgc ttcgacgtta gcgaagaagc ggaagttgcc
    12781 gttcgtcatc acgccgagac gaaactccac attgatgctc gcgcccggcg caagcggcgt
    12841 cgagagcgtg atcgagttga cgctcagagt ggagttgatg ccgccgcctt ccggttgatc
    12901 gaacgattgc ccgagcgtca ggccgcgtag aaagaccgac tggttgttgg tgtccgtgac
    12961 ggtcacgtcg ctcgaagaga tggcgcgcag gtcggcctga ttcgcctgct cgttgccctt
    13021 cgtcgagaga tcgacgacgc ggaagcgcag ccggttgatc ttctgcccgg tgacgttggt
    13081 gaatttgcgc cggagcagca gcgtgccgaa ggccgcgttt tgcgcattgg cgttctgcgc
    13141 ggtacgcacg cgcgcgcacg cgctcgccgg cgagccgaag cctgcgcatt gggtgtcgat
    13201 gagcgacgac ttgatggtcg cgttgcgttg caggtggctg cctgagttct gcgggccggg
    13261 cgcgccgaga attgattgaa tcccgccgaa catggctgcg ctggtggaaa ccagcacgaa
    13321 gtcctgtgcg ttgtcgcccg tgtcctgcgg cgtgccgctc gtcaacttgc ggacgaaact
    13381 gtactgggcg ttgtccgtcg gcatcgaggg tagtcccgtc ccttcacgat aggccacgtt
    13441 agtctccgcc tcgaacccgg cggcatcgag gatgttgccg ggcagttcgt agttcgcggc
    13501 ggtggcggtg cgcacgagcg cgaggccgga attatcgggg atgtcgccgc cgtagaacgc
    13561 atcgggcgag gcataggcgg tgaggctata gccgctgccg ttgccgacga ggaagtggcc
    13621 gcgcggcggg atgatcgtgc cgcgctgaat gacataacgc acggcgggtg cgagcgtgtt
    13681 ggacgaagtg atcgcccaac cgttcgatcc gtctgccgtg ttgacgacga aggttgaatt
    13741 cgtgttgtta taaatttcga tgaactcgtc gcgcgcgccg cccgtcgcgg ggtcgccgcc
    13801 gatggggaag gggccgcgca ggcgaaactc gctgatgagc agttggcctg cttcgggcag
    13861 gacggtgcgc gtgaagttga ccgtttgatt gccctgataa gaggcgaagg tacggctggc
    13921 cggatagaag gtcgaattgc cttcgacggc ggtgatggta tgggtgctgc ccgcgacgag
    13981 tccttcgatg agatagtggc cggtggcgtc ggtggtcgtc gtggccgtcc ccgcgcccgt
    14041 caggttgatg gtcacgccgg gcagcggatt gccctccatg tcaacgacct gtccttcgat
    14101 gaagagcgaa ttggactgga cgccttgcgc gacgagcatc tggccgttgc ggagtccgcc
    14161 cgtgtgacat tcgtactgcg tgaacgcgcc cgccgggtcg cccgccgccg cgactctctg
    14221 cacgccgacc gtcgcttcga ggccgttgcc cggcacggtg ccataaatga tgtcgaagcg
    14281 cgtctggcct tcgtagaggc gaacttcaaa attgaggctg cccgccggtt tgccgaactg
    14341 ggaggcgcgc cattcgatat tgaagatgcg gttgggagcc gtgcccgaga cggaagtgaa
    14401 cacgccttcg cccgcgccgc cgatggtcag gtcggtgaaa tgcgggagga tcgagtagct
    14461 gaaatgaagg agcggcaggc agagttcgtt cagcccgtag tcggtgttgg cagtcacaaa
    14521 ctggagggtg ccgtttgaag tgacgttcgc ctgcgtgtag ctgttcgtgt agaacgtgta
    14581 agcgaaaggc agattgacgg tcgccacgca gtcgtcgccg ggaaccgagc cgcaggcgaa
    14641 attcgtgacg ggcgtgttgc cgggcacgac cgccgccccg ttcgtagagg tgaagcgata
    14701 ctggttcacc gcctgcgccg atgcgcccga ttgcgcgccg tagcagaaca ggcagagcgc
    14761 gaagcccaga agctgcgccg tcaagcgggc gcgagaaaat tgcacgaggg aacgcatcac
    14821 ttactacctc ctgaattatc cgtcgagtat ttaactgaaa gtttttgacc gggcgagcca
    14881 tcaggcaagt caggctgccc acacttgttg cgagaaatta tctgccgggg gaaagtggaa
    14941 agctaacaca ggacgaggga agaaaggaag tgatgagtga agagtgatga gtgatgagtg
    15001 aaagacaaga ttgctgcttt tactctccac tcattactca acctcagtct tgagttgatc
    15061 aggccgagag caa
//
LOCUS       DASWNS010000277        15743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_451301, whole genome shotgun sequence.
ACCESSION   DASWNS010000277 DASWNS010000000
VERSION     DASWNS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15743
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451301"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VGB76_09845"
     CDS             complement(<1..555)
                     /locus_tag="VGB76_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0099245.1"
                     /translation="MVSHGLARRLGLFDATMLVMGGIVGAGIFLNPAEVARQVHTPAL
                     IIGAWLLGGLIALAGAFVYAELAARRPEVGGQYAYLRDAYHPTVAFLYGWSLLLVIQS
                     GGMAAVAITFALYFRQLTGAPLSEGAIAAIALTLLTVINCLGVRAGSNVQSALMVLKI
                     AAIAALVFCGLWFSTGAQSSFAAAP"
     gene            1265..5359
                     /locus_tag="VGB76_09850"
     CDS             1265..5359
                     /locus_tag="VGB76_09850"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0099246.1"
                     /translation="MKRNLPLAFAVILTLSLMPSLLDWNVGAQTSPAPQTKDSSATGE
                     RGAAQERERSPRSQDEKGSLKTDRKRKPAPADKAVENDGDLPNFMNGKVNRKEYLRKR
                     AEQIGMLRGRSDAAKLKDSPRIRAIQELERQESSLRKAAAEGRVQPLISNTSWTPIGP
                     APLPNGQTDTVTTPVNGRVTCVAIHPTNIDIVYVGTAQSGVYRTLNGGQTWTPILDSA
                     QTLAIGSIAIDPITPTTIFVGTGEGNLGLDNFEGVGIYRVLNADTAPVVQGPFESRTN
                     GGGGHAFVGTAINKIIIDPTNNNNMFVGNTLAGSGMSGEGTCCGGTNPASAFIGLYFS
                     ANAQAATPTWIRVGTLPGGGLAGITDISIDPTNANVLVLNQQDFFDASLSGVYRSTNA
                     LSGATATFTRVINYAGTFNNAKLASYKPGAANAVFYLASEEGANEGAVRRSTDGGATW
                     TAVGALGGTGFCSPQCFYDIAVDVDPGATTATTDDIVYLGGAAGNTIFQKSTNGAATF
                     TPSQRGLHADTHAIEIAPSNPNVVYTGDDGGIFRSYNKAADWNSLNNTGFNALQFQSL
                     ALHPTDRYFTIGGTQDNGTEFLQPDGNTQLPYNSWRRADFGDGGFAQIDQSGGSSTSV
                     VTMYHTYFNATGAVLGFARVSSTACATEGQWAFKGFGAPPGFVNACGDAEGPNGITPS
                     DNVLFYAPLELGPGTPNTVYYGTDRLYRSINRGDTMTLASQAPLSPIAGNPTIGSPIS
                     AIGISPQDDNVRLVGLANGALFFTTTGLPVLTSLDPVGAGSVIPDKYVGRVRFDPTNA
                     NTAYITLNGFTGAQNHVYRVTNLSTTPVITNISGTGATGIPDIPVNAFAVDPLNPTSL
                     YAGTDIGVYISTNGGTTWAPYGTGLPRVAVFDMEIHSQHRLLRIATHGKGMWDIGLVP
                     GSTLTISGTIRDASSNPISGAIVRLNNNAATEVTTTAAGTYTFTGLAVGSNNTVSVTK
                     AATRFAPAYRTFNDMSTNQTGDFTGTPAPGGTPPTTGAILISEFRTEGPAGATDEFVE
                     LYNNTDAPVTVQASDATAGWAVYALDATSTTSVEIAIIPNGTVIPARGHFLLTGSAYS
                     LRTLTVSDFNYNADIPNNTGVALFRTTSISPFNAADRLDAIGFFGVAGATPDMFREGS
                     GITAPSTTGLQHSYVRRTLANGNPRDTNENATDVALVSTNAAPGSVLGAPGPENVFSP
                     ITRTGRYTVGVVDPSQCTTCQPNYERVGSDYGVNKAFGTLTLRRKFTNNTGQTATRLR
                     FRVIDITTLNTPGVGADLRLVDSTDTIRGAFTIRGTQLELPMQPLGGGYNSSATVVLP
                     DGGLTSTSGGTCPAGATCSLDVQFTLGVQVQGAFRFIVNIESLP"
     gene            complement(5500..6378)
                     /locus_tag="VGB76_09855"
     CDS             complement(5500..6378)
                     /locus_tag="VGB76_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0099247.1"
                     /translation="MSNIIYRAAVEADIPATLDLFLEAVAEIYQRHNMHMPLPPRPSV
                     EVAYRHIFRTGIFRVAESDGRLICICHAVVRDELWFLSGFWARPELQARGVGGKLIRE
                     VWREGAEAGAKKFFVWSSMDHTAIASYLKMGMLPGYQIFTFSGRPDALPDAPTGYEVE
                     PLSLEAACDIDRAIRETRRETDHHFWLSEVKLEGRQVRRRDGRLAGYYYFKGEALAPV
                     AWMERGDAEAVLTLACRDAVAQADAISLRVPGINHDAIRFALACGLRLNAMAHLFTSA
                     PFGRMEQYLASGPTLF"
     gene            6676..8736
                     /locus_tag="VGB76_09860"
     CDS             6676..8736
                     /locus_tag="VGB76_09860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0099248.1"
                     /translation="MKRATSPPALLWVLLIVLTFTGDVQVVTAQSKKLTIEDALALQE
                     VGAPQWSPDGRLIAFPVSEWNKKENRRDSHIHIIPAAGGASYKLTNGERGESAPQWSP
                     DSTRIAFLANRSAPAPDAPGRNQIWVIPVAGGEAERVTDEEAGVAQFRWSPDGKQLAY
                     VVRDTPQDKAERDKRKKDKFDAIVVDSGFIYSHLWTIKLDTKEKKRVTEGSFSVTDPQ
                     WSPDGRWVAYTMSKGGAQESSFTDISDERNTDLYVAPATGGAVRQLTANPGPDYAPRF
                     SPDGKWLAYLSNSDPASWAAKTDLMLISTAAAEGNSSADTAPRNLTGKYNDSVNGAAK
                     WSPSGDALYASGAEGVYGQLLKIGVAGGEPKTIFASKGAYSGVDLSRDGSTLAFVFND
                     AKSPNDIWTASSAGRDARQLTNFNPGLKEFALVPTEVIRWKAPDGLEIEGLLVKPLNY
                     EQGKRYPLILQIHGGPYAQFAYGLNSRAQIFAANGYAVLLPNPRGSTGYGNKFTTANI
                     GDWGGKDYQDLMTGVDEVIKMGLADPERLAVMGGSYGGFMTFWVITQTNRFKAAIGHA
                     GISDWYSFYGQSDIPGLMEYGFGGMPWTAREVYEKWSPVRYADRVKTPLMITHGEQDR
                     RVPIAQAEQYYRALRKRGVETVFVRYPREGHGIAEPNHQIDLVRRQLEWFDKHLKLNA
                     TASR"
     gene            8797..12126
                     /locus_tag="VGB76_09865"
     CDS             8797..12126
                     /locus_tag="VGB76_09865"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099249.1"
                     /translation="MMRKQRLQELVVTLLRLALSASLAGIFFAQAQAQQTATPTPQTP
                     QPRDATSPLKLLQWRSIGPFRGGRVTAVAGVASQPFVYYFGATGGGVWKTTDGGANWE
                     PISDGAVFGTGSVGAIGICEADPNVIYVGMGESPVRGNVSHGDGVYKSMDAGKTWKRV
                     GLEDTRQIGRVRVHPRNPDIIYVAALGHLFGANEQRGVFRSKDGGKTWEKILYRSNKA
                     GAIDLTFDPSNANILYAGFWEVLRTPWSLESGGAGGGLFKSTDGGDTWTEITRNPGLP
                     RGLVGKVGITVSPANPERVWAIVEAEDGGVFRSDNGGRTWTKTNEQRNLRQRAWYYTR
                     IYADTQNADTVYVLNTGFYKSNDGGRTFSSISVPHGDNHDLWIAPNDAQRMINSNDGG
                     ANVSFNGGRTWSEQDQATAQFYRVALDNNFPYNIYGAQQDNSTVRIASRTTDFGITER
                     DWWDVGGGESGWIAPSPKDSNIVFAGSYGGLITRYDHRTGQLRNVDVWPDNPMGAGAE
                     AMKYRFQWNFPILFSPHDPNLLYTAGNILFKSTNEGQSWTAISPDLTRNDKTKQGASG
                     GPITKDNTSVEYYDTIFTVSESPVKAGVIWTGSDDGLVYVTRDSGGKWDNVTPRGIPE
                     WIQINSLDASPHDAGAAYVAATMYKLDDFRPYLYKTNDYGKTWKKITNGIPDTAFTRV
                     IREDPNRRGLLYAGTETGMYVSFNDGESWQPLQLNLPVVPITDLAVHKGERDLVAATQ
                     GRSFWILDDLSVLHQLMDAGGANLASMETRLFKPEDPYRIPGGGGGGQLPATATIGSN
                     PASGAVVYYYLKSKPKTDVVLEFLDATGKTIRSFTAKAPAPQPSPAPGSAAVQTPPEQ
                     PQAASGEESSFFAGGGGSAPRVTTEVGLNRFVWNMRHADAVRFPGMILWAGSTTGPRV
                     IPGRYQVRLTADGKTYTETFEVRKDPRLTTTDADFARQLDLQLKIQDKLTETHNAIIS
                     IREARTQINDLLKRVQGQPNFKPISDAAKSLNGKMTDIEEALYQTKNQSSQDPLNYPI
                     RLNNKLAALGGVVSSADSAPTEQSYAVYEDLSAKINAQLQKLDVVMKSDLPAFNALVR
                     EQNIPAVILKPTAATPQGVRP"
     gene            12134..13036
                     /locus_tag="VGB76_09870"
     CDS             12134..13036
                     /locus_tag="VGB76_09870"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEY0099250.1"
                     /translation="MFNIDLSLEVAAAIEAGRPVVALESTVIAHGLPRPANLETARRL
                     EQIVRAEGAIPATIAILGGKFCVGLSDEQLAHIAASRDVKKISCRDLPIAAARLWDGA
                     TTVASTIWIAERAGIGVFATGGIGGVHRGNLPDVSADLPELARTRLVVVCSGAKIVLD
                     LPATREWLETYGVTVAGYGCDELPAFYTQRSGLHVDVRVDTPEEASAIARAQRQLGKD
                     GALLVCVPVPAASEVAADVLQETLEAALAEAAGAEINGRELTPFLLSRMSERSGGATL
                     KANVALLENNARIAAQIAGAVARQ"
     gene            13132..13791
                     /gene="nth"
                     /locus_tag="VGB76_09875"
     CDS             13132..13791
                     /gene="nth"
                     /locus_tag="VGB76_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY0099251.1"
                     /translation="MVEVEEKKRAAKIIGKLKRAYPQARCSLNYSTPLELLIATILSA
                     QCTDERVNLVTTDLFRKYRGADDYVRAAPAELERDIHSTGFFRNKAKAIQGACRMINE
                     EYGGRVPETLEELIALPGVARKTANVVLGNAYGIASGVVVDTHVSRVAQRLKLTGHEQ
                     PEKIERDLIELVPRADWIIFPHLLIAHGRATCKARTPLCAECPVEPLCPSSMKGMKDE
                     G"
     gene            complement(13847..14797)
                     /locus_tag="VGB76_09880"
     CDS             complement(13847..14797)
                     /locus_tag="VGB76_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099252.1"
                     /translation="MLNSLALRPFFNERENSSVQISIPVPPAFNFKRTAKSHGWRSLL
                     PFEFDDTVWSITRVLSLPAATPVTVHVTSSDDALQVNIPRRTLNKRDTAQITRDVRHM
                     FRLDDEMQVFYAAVADDPNFSWIVREGAGRLLRSPTVYEDLVKTICTTNCSWALTMKM
                     VEGMVKHLGREAQDGRRAFPTPEAMAGQPESFYRDVVRAGYRAGYFTELAGRVASGEL
                     DVESWLTSELSTAELKLEMKRVKGVGEYAADNLLKLLGRYDCLALDSWVRATFARLHN
                     KGRTADDRKIARHYARFREWRGLALWCDITRDWIEAKDEG"
     gene            complement(14837..14908)
                     /locus_tag="VGB76_09885"
     tRNA            complement(14837..14908)
                     /locus_tag="VGB76_09885"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14873..14875),aa:Ile,seq:gat)
     gene            complement(15033..15108)
                     /locus_tag="VGB76_09890"
     tRNA            complement(15033..15108)
                     /locus_tag="VGB76_09890"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15073..15075),aa:Lys,seq:ttt)
     gene            complement(15117..15191)
                     /locus_tag="VGB76_09895"
     tRNA            complement(15117..15191)
                     /locus_tag="VGB76_09895"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15157..15159),aa:Asn,seq:gtt)
     gene            complement(15306..15379)
                     /locus_tag="VGB76_09900"
     tRNA            complement(15306..15379)
                     /locus_tag="VGB76_09900"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15345..15347),aa:Met,seq:cat)
     gene            complement(15391..15468)
                     /locus_tag="VGB76_09905"
     tRNA            complement(15391..15468)
                     /locus_tag="VGB76_09905"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15431..15433),aa:Thr,seq:tgt)
     gene            complement(15479..15552)
                     /locus_tag="VGB76_09910"
     tRNA            complement(15479..15552)
                     /locus_tag="VGB76_09910"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15516..15518),aa:Ala,seq:tgc)
     gene            complement(15577..15649)
                     /locus_tag="VGB76_09915"
     tRNA            complement(15577..15649)
                     /locus_tag="VGB76_09915"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15614..15616),aa:Met,seq:cat)
BASE COUNT         3242 a         5084 c         4673 g         2744 t
ORIGIN      
        1 gggcgcggcg gcgaagctac tttgcgcgcc ggtcgaaaac cacaggccgc aaaagacgag
       61 cgcggcgatg gctgcgatct tcaacaccat gagcgcgctc tgcacgttgc ttccggcgcg
      121 gacgcccaga cagttgatga cggttaaaag cgtgagcgcg atggcggcga ttgcgccttc
      181 ggagaggggc gcgcccgtga gttgtctaaa gtagagcgcg aaggtgatgg cgacggcggc
      241 catcccgccc gattgaatga cgagcagcaa agaccagcca tagagaaacg cgaccgtggg
      301 atgatacgcg tcgcgcaggt aagcatactg gccgccgacc tccggcctgc gcgccgcgag
      361 ttcggcatag acgaacgccc cggccagagc gatcagcccg cccagcagcc acgcgccgat
      421 gatgagcgcg ggcgtgtgca cctgccgcgc cacttccgcc gggttgagga agatgcccgc
      481 gccgacaata ccgcccatga cgagcatcgt cgcatcgaac agccccagac ggcgcgcgag
      541 tccgtgcgaa accacttttg ttggctgcga cgacacgttc ggctgcgact ccactcagga
      601 atgaacccgt ttcggctggc ctttaagttc catctttcgc ttgagagtaa agctgtcgaa
      661 aattctctgt gccgctatgg tagcggctgc gcgttgcgat gtctaaagta aagtgtctgt
      721 atgggctacg ggagtttccg gttgacagta gaaacatcaa aacgtaaagt agagcgtgtc
      781 cggggcgatc ttcattcttg cttaagtttc accgtacgat gtcaggttcg accccgatca
      841 ttcattaaca ttgatcttgc tggcgttgcc ggccctgagc cgcacattcc gccataacat
      901 ccaaaacgca tgcctcttgc tccgcttctt caaattacgg aagtagtttg cgttaatcgc
      961 actcgctcgg cgatgagtcg agagttgaaa gcattttaag tgattacttc ccactgctta
     1021 attgacgcgg agaatcgtcg tcgagacgct cgtatcgagt ttacatactg tcaacacggg
     1081 ttcccttacc agccccaaac tcacacctca aacagttgcc tgagcgacac acgcctctgc
     1141 tgtggcgtcg gctttgaaca gacctgaaat agcgcgccgc tgctgcgtcc acgaagaagt
     1201 gcggggcatc cgcttgcgcc ttgcagagac actttaacag gattacgcct ccggagaata
     1261 aaacatgaaa cgaaacttgc cgctcgcctt tgcggtgatt ttaacattgt ccttaatgcc
     1321 ttcgctgctc gactggaacg tgggcgcgca aacgtcgccc gccccgcaaa ctaaagatag
     1381 ctccgctacg ggcgagcggg gagcggcgca ggagcgcgaa cgctcgcctc gttcgcagga
     1441 cgagaagggc agcctgaaga ccgaccgcaa gagaaaaccc gcaccggcag ataaggccgt
     1501 tgagaacgac ggcgacctcc ccaacttcat gaacgggaag gtgaaccgca aggaatatct
     1561 acgcaagcgc gccgagcaga tcggcatgct gcgcggtcgt tcggacgccg cgaaactgaa
     1621 agacagccct cgcatcaggg cgattcagga attggaacga caggaatcga gcctgcgcaa
     1681 ggccgccgcc gaagggcgcg tgcagccgct catcagcaat acgagttgga cgccgattgg
     1741 acccgccccc ctccccaacg ggcagaccga cacggtgacc actccggtca acgggcgcgt
     1801 cacctgcgtc gccatccacc cgacgaacat cgacatcgtc tatgtcggca cggcgcagtc
     1861 cggcgtctat cgcacgctta acggcgggca gacgtggacg ccgattctcg actcggcgca
     1921 gacgctcgcc atcggctcga ttgccatcga ccccatcacg ccgacgacca tcttcgtcgg
     1981 cacgggcgaa ggcaatctcg gactcgacaa ctttgaaggc gtcggcatct atcgcgtcct
     2041 gaacgccgat accgctcccg tcgtgcaggg tcccttcgag tcgcgcacga acggcggcgg
     2101 cggccatgcc ttcgtcggca cggccatcaa caagatcatc atcgacccga ccaacaacaa
     2161 caacatgttc gtcggcaata cgctggcggg cagcggcatg agcggcgaag gcacttgctg
     2221 cggcggcacc aacccggcgt cggccttcat cggactctac ttctcggcca acgcgcaggc
     2281 ggccacgccc acctggatac gcgtcggcac cctgccgggc ggcggcttag ccggtatcac
     2341 cgacatttcc attgacccga ccaacgccaa cgtcctcgtc ctcaaccagc aggacttctt
     2401 cgacgcgagc ttgagcggcg tctaccgttc gaccaacgcg ctctccggcg caacggcgac
     2461 cttcacgcgg gtcatcaact acgcgggcac gttcaacaac gcgaagctcg ccagctacaa
     2521 accgggcgcg gccaacgccg tcttctacct cgcgtccgaa gagggcgcga acgagggcgc
     2581 cgtgcgccgc tcgaccgacg gcggcgcgac gtggactgcc gtgggcgcgc tcggcggcac
     2641 gggcttctgt agcccgcagt gcttctacga catcgccgtg gatgttgacc ccggcgcgac
     2701 caccgcgacc accgacgaca tcgtctatct cggcggcgcg gcgggcaaca ccatcttcca
     2761 gaaatcgacg aacggcgcgg ccaccttcac gccctcgcag cgcggcttgc acgccgacac
     2821 gcacgccatc gagatcgcgc cttcgaaccc gaacgtcgtc tatacgggcg acgacggcgg
     2881 catcttccgt tcttataaca aggccgccga ctggaacagt ctcaacaaca cgggcttcaa
     2941 cgccctccag ttccagagcc tcgccctgca cccgaccgac cgttatttca ccatcggcgg
     3001 cacgcaggac aacggcacgg agtttctcca accggacggt aacacgcagt tgccctacaa
     3061 cagttggcga cgcgccgact tcggcgacgg cggcttcgca cagattgacc agagcggcgg
     3121 ctcgtccacg agcgtcgtca cgatgtatca cacctacttc aacgccaccg gcgcggtgct
     3181 cggcttcgca cgcgtctcca gtacggcttg cgccaccgaa ggccagtggg cgttcaaagg
     3241 cttcggcgca cctccgggat tcgtcaacgc ctgcggcgac gcggaaggac caaacggcat
     3301 cacgccctcg gataacgtcc tcttctacgc gccgctcgaa cttggacccg gcacgcccaa
     3361 caccgtctac tacggcacag atcgactcta tcgctcgatc aatcgtggcg acaccatgac
     3421 gctcgccagt caggctccgc tctcacctat tgccggaaac cccaccatcg gctcgcccat
     3481 cagcgcaatc ggcatctcgc cgcaggacga caacgtgcgc ctcgtcggtc ttgcgaacgg
     3541 cgcgctcttc ttcaccacga ccggcctccc ggtgttaaca agcctcgatc cggtcggcgc
     3601 aggcagcgtc atccccgaca agtacgtcgg gcgcgtgcgc ttcgacccga ccaacgccaa
     3661 caccgcctac attacgctca acggtttcac cggagcgcag aaccacgtct atcgcgtgac
     3721 gaacttgagc accacgcccg tgatcacgaa catctccggc acgggcgcaa ccggcatccc
     3781 tgatattcca gtcaacgcct tcgccgtcga tccactgaat cccacgagcc tctacgcggg
     3841 cacggacatc ggcgtctata tctccaccaa cggcggcacg acctgggcgc cttacggtac
     3901 gggcttgccg cgcgtggccg tcttcgacat ggagattcac agccagcatc gcctcctgcg
     3961 catcgccacg cacggcaaag gcatgtggga catcggcctc gttccgggca gcacgctcac
     4021 catcagcggc acgatccgcg atgcgagcag caacccgatc tccggcgcaa tcgtgcgact
     4081 caacaacaac gctgcgacgg aagtgacgac gaccgccgcc ggaacttaca cgttcaccgg
     4141 cctcgccgtc ggcagcaaca acaccgtgtc ggtgacgaaa gccgccacgc gattcgcgcc
     4201 cgcctaccgg acgttcaacg acatgtccac gaatcagacg ggcgacttca ccggcacacc
     4261 cgcaccgggc ggcaccccgc caactacggg cgcgatcttg atctccgagt ttcgcacgga
     4321 aggtccggcg ggtgcgaccg acgagttcgt cgagttgtac aacaacacag acgcgcccgt
     4381 caccgtgcag gcttcggacg cgactgctgg ctgggcggtc tatgcgctcg acgcgaccag
     4441 cactacgagc gtcgagatcg ccatcatccc gaacggcacg gtcattccgg cgcgcggcca
     4501 cttcctgctt actgggtcgg cctacagcct gagaacgctc accgtcagcg atttcaacta
     4561 caacgccgac atccccaaca acacgggcgt cgcgctcttc cggacgactt caatctcgcc
     4621 cttcaacgcg gctgaccgtc tcgacgccat aggcttcttc ggcgtcgccg gggcgacccc
     4681 ggatatgttc agggaaggca gcgggatcac tgcgccgagc acgaccggac tccagcacag
     4741 ttacgtgcgc cgcactctgg cgaacggaaa cccgcgcgac accaacgaaa acgcaaccga
     4801 cgtggccttg gtttcgacga acgccgcgcc cggttcggtc ttgggtgcgc cggggccgga
     4861 gaacgtcttc agcccgatca cgcgcacggg tcgctacacc gtcggggtgg ttgatccgtc
     4921 gcagtgcact acctgtcaac ctaactacga gcgcgtcggg tctgactacg gagtcaacaa
     4981 agccttcggc acgctcacgc tccggcgcaa gttcaccaac aacaccggac agacggccac
     5041 gcgccttcgc ttccgcgtca ttgacatcac gacgctgaac acgcccggcg tcggggctga
     5101 cctccggctg gtggattcca cggacaccat ccgtggcgcg ttcaccattc gcggcacgca
     5161 actcgaactg ccgatgcaac cgctcggcgg cggctataac tcttccgcca cggttgttct
     5221 ccccgacggc ggtttgactt cgacgagcgg aggcacctgc cccgccgggg caacctgctc
     5281 gctcgatgtt cagttcacgc tcggcgtaca ggtgcagggc gctttccgct tcatcgtcaa
     5341 catcgagtca ttgccttaac cacagcacac aggggcggga cgcactaacg gcaacaccaa
     5401 ctcccgcccc tctacccaac gaaacgagcg gcggggcgat ccgtggagga tcgcccggcc
     5461 actcgtcgtt cacggctatt tctgagctac agagaactgc taaaagagcg tgggtcccga
     5521 ggcgagatat tgttccatgc gaccgaaggg ggcggaagta aacaggtgcg ccatggcgtt
     5581 caggcgcagt ccgcacgcga gcgcgaaacg gatcgcgtcg tgattgatgc cgggcacgcg
     5641 tagcgagatg gcatcggcct gcgcgacggc atccctgcaa gccaacgtca agacggcttc
     5701 cgcgtcgccg cgctccatcc atgcgacggg cgcgagtgct tcgcccttga aatagtagta
     5761 gccagccaga cgcccatcgc gacgccgcac ctgtcgccct tcgagcttaa cttccgagag
     5821 ccagaaatga tgatccgttt cgcgccgcgt ctcgcgtatc gctcgatcta tgtcgcacgc
     5881 ggcttcgagt gagaggggtt caacctcgta acccgtcggc gcgtccggca gagcgtccgg
     5941 cctgccgctg aaggtgaaga tttgataacc gggcagcatc cccatcttga gataactggc
     6001 gattgccgta tggtccattg acgaccagac gaagaacttt ttcgcgcccg cctccgctcc
     6061 ctcgcgccac acttctctga tcaacttccc gccgacgccg cgcgcctgca actccggcct
     6121 cgcccagaag cccgaaagaa accagagttc gtcgcgcacg acggcgtggc aaatgcagat
     6181 gagcctgccg tcgctctccg ccacgcgaaa aattcccgtc cgaaaaatgt gccggtacgc
     6241 gacttcaacc gacggtcgcg gcggcagcgg catgtgcatg ttgtgacgtt gataaatctc
     6301 cgcgaccgct tcgagaaaaa gatcgagcgt ggcggggatg tccgcttcca cggcggcgcg
     6361 atatataatg ttcgacatag attgattttc gcccaacccg accggcaaaa ttttagatga
     6421 agaacacgaa agcgcgtaga cccggcgcgc gccccgcatt gtcgcttccg cgcctgaatt
     6481 cggcgcgata aaattcgcgc acaccttttt ccggcgcggc gcagcaggat aaaagatttt
     6541 tcgagcattt cccagaggga tgcgttataa atagtttttc ccgcgatgta cccaattcaa
     6601 aaattagaga tcattgctca agtcagaagt ggccttgaag ttcgcttact ccgcacaaga
     6661 caaggaaacg aagttatgaa acgtgctacc tcaccccccg ctctgttgtg ggtgttgctc
     6721 attgtcctga cgtttacggg cgacgtgcag gtcgtcacgg cgcagagcaa gaagttgacg
     6781 attgaagacg cgctcgcctt gcaggaagtg ggcgcgccgc agtggtcacc cgatggacga
     6841 ctgatcgcct ttccggtcag cgagtggaac aaaaaagaga accgccgcga ttcgcacatc
     6901 cacatcatcc cggcggctgg cggcgcgtct tacaaactga cgaacggcga gcgcggcgag
     6961 agcgcgccgc agtggtcgcc agactccacg cgcatcgcct ttctcgccaa ccgcagcgcg
     7021 cccgcccccg acgcccccgg gcgcaatcaa atctgggtca tccccgtcgc gggcggagag
     7081 gccgaacgcg tgacggatga agaggcgggc gtggcgcagt ttcgctggtc gcccgacggc
     7141 aagcaactcg cctacgtcgt gcgcgacacg ccgcaggaca aggccgaacg cgacaagcga
     7201 aagaaggata agtttgacgc catcgtcgtc gatagcggct tcatctactc gcacctgtgg
     7261 acgatcaaac tcgacacgaa agagaagaag cgcgtcacgg aaggctcgtt ctccgtcacc
     7321 gacccgcagt ggtcgcctga tggccgctgg gtcgcctaca cgatgagcaa gggcggcgct
     7381 caggaatcgt ccttcacgga catctccgac gagcgcaaca cagacctcta cgtcgcgcct
     7441 gcgacgggcg gcgcggtgcg acaactgacc gcgaaccccg gcccggacta tgcgccgcgc
     7501 ttctcgcccg acgggaagtg gctcgcctac ctctcgaaca gcgaccccgc ctcgtgggct
     7561 gcgaagaccg acctcatgct catctcgacc gccgctgccg agggaaactc ctccgccgat
     7621 actgcgccgc gcaacctgac gggcaagtac aacgattccg tcaacggcgc ggcgaagtgg
     7681 tcgccgtcgg gcgatgctct gtacgcgtcg ggcgcagagg gcgtatatgg gcagttgctc
     7741 aaaattggcg tcgcgggcgg cgagccgaaa acgatcttcg ccagcaaggg cgcgtatagc
     7801 ggcgtcgatc tctcgcgcga cgggagcacg ctggccttcg tcttcaacga cgcgaaatct
     7861 ccaaacgaca tctggaccgc atcgagcgcg ggacgcgacg cacggcaact caccaacttc
     7921 aacccggggc ttaaagagtt cgcgctcgtg cccacggaag ttatccgctg gaaagccccc
     7981 gacggcttgg agatcgaagg cttgctcgtc aaacctttga actacgaaca gggcaaacgc
     8041 tacccgctca tcttgcagat tcacggcggc ccttacgcac agttcgccta cggactcaac
     8101 tcgcgcgcgc aaatcttcgc cgccaacggt tacgccgtcc tcctgccgaa cccgcgcggc
     8161 tcaaccggct acggcaacaa atttacgacc gccaacatcg gcgactgggg cggcaaggat
     8221 tatcaagatt tgatgacggg cgtggacgaa gtgatcaaga tgggcttggc cgacccggag
     8281 aggctcgccg tgatgggcgg ttcatacggc ggcttcatga ccttctgggt catcacgcag
     8341 acgaaccgtt tcaaggccgc catcggtcac gcgggcatca gcgattggta cagcttctac
     8401 gggcagagcg acattcccgg cctcatggaa tacggcttcg gcggcatgcc ttggacggcg
     8461 cgcgaagtct atgagaagtg gtcgcccgta cgctacgcgg atcgtgtgaa gacgcctctg
     8521 atgatcacgc acggcgagca agaccgccgc gtgcccatcg cgcaggccga gcagtattat
     8581 cgcgcgctga gaaagcgcgg cgtcgaaacc gtcttcgtac gctacccgcg cgaagggcac
     8641 ggcatcgccg aaccgaatca ccagatcgat ctcgtgcgcc gccagttgga gtggttcgac
     8701 aagcatttga agttaaacgc aacggcgagc cgttagtcgt caagtgttga tctaacttgc
     8761 cgttcagact gaatattgag gagcgcgttc gattcgatga tgagaaaaca acggctgcaa
     8821 gaacttgttg tcaccctgct gcgtctggcg ttgtctgcgt cgctggcggg tatcttcttt
     8881 gctcaggcac aggcgcagca aacggcgaca ccaacgccgc agacgccgca gcccagagat
     8941 gccaccagcc cgctcaaact cttgcagtgg cgcagcatcg gaccatttcg cggcgggcgc
     9001 gtaacggcgg tggcgggcgt ggcctcgcag ccttttgttt attacttcgg cgcgacgggc
     9061 ggcggcgtct ggaagactac ggacggcggc gcgaactggg aacccatttc tgatggcgcg
     9121 gtcttcggca caggctcggt cggcgcgatt ggcatctgcg aagccgaccc gaacgtgatc
     9181 tacgtcggca tgggcgagtc gcccgtgcgc ggcaatgtct cgcatggcga cggcgtctac
     9241 aagtctatgg acgcggggaa gacctggaag cgcgtcggcc tcgaagacac gcggcagatc
     9301 gggcgcgtgc gcgtccaccc gcgcaaccct gacatcatct atgtcgccgc gctcggacat
     9361 ctcttcgggg caaacgaaca gcgcggcgtc tttcgttcaa aggacggcgg gaagacttgg
     9421 gagaagattc tttaccggag caacaaggcg ggcgcgattg atctgacctt cgacccgtcg
     9481 aacgccaaca tactctacgc gggcttctgg gaagtgttgc gcacgccgtg gagtctggag
     9541 agcggcggcg cgggcggcgg cctcttcaag tcaaccgacg gcggcgacac gtggacggag
     9601 atcacgcgca accccggcct gccgcgcggc ctcgtgggaa aggtcggcat caccgtctcg
     9661 cccgcgaacc cggaacgcgt ctgggctatc gtcgaagcgg aagacggcgg cgtgtttcgt
     9721 tcagacaacg gcgggcgcac ctggacgaag acgaacgaac aacgaaacct gcgacagcgc
     9781 gcttggtatt acacgcgcat ctacgccgac acgcagaacg ccgatacggt ctatgtcttg
     9841 aatacgggct tctataaatc gaacgacggc gggcgcacct tctcgtccat ctccgtgccg
     9901 cacggcgaca atcacgatct ctggatcgcg ccgaacgatg cgcagcgcat gatcaactcg
     9961 aacgacggcg gcgcgaacgt ctctttcaac ggcgggcgca cctggtcgga gcaggatcag
    10021 gcgacggcgc agttttaccg cgtcgcgctc gacaataatt tcccatacaa catctacggc
    10081 gcgcagcagg acaactccac cgtccgcatc gcctcgcgca ccaccgattt cggcatcacc
    10141 gagcgcgact ggtgggacgt gggcggcggc gaatccggct ggatcgcccc ttcgccgaaa
    10201 gattcaaaca tcgtcttcgc gggttcttat ggcggactca tcacgcgcta cgatcatcgc
    10261 acggggcaac tccgcaacgt ggacgtgtgg ccggacaatc cgatgggcgc gggcgcagag
    10321 gcgatgaagt atcgcttcca gtggaacttc ccgatcctct tctcgccgca cgatccgaac
    10381 ctgctctaca ccgccggaaa catcctcttc aaatccacga acgaggggca gagttggacg
    10441 gcgatcagtc cagacctgac gcgcaacgat aaaacgaagc agggtgcttc gggcggtcct
    10501 atcacgaagg acaacacgag cgtcgagtat tacgacacga tcttcaccgt cagcgagtcg
    10561 cccgtcaaag cgggcgtcat ctggaccggc tcggacgacg gcctcgttta cgtcacgcgc
    10621 gactccgggg gcaagtggga caacgttacg ccgcgcggca tccccgaatg gattcagatc
    10681 aactcgctcg acgcttcgcc gcacgacgcg ggcgcggcct acgtcgccgc gacgatgtac
    10741 aaactcgacg acttccgtcc ctatctctac aagacgaacg actacggcaa gacctggaag
    10801 aagatcacga acggcatccc cgacacagcc ttcacgcgcg tcattcgcga agacccgaac
    10861 cgtcgcggcc tgctctacgc cgggacggag acgggcatgt acgtctcctt caacgacggc
    10921 gagagctggc aaccgctcca actcaacctg cccgtcgtgc ccatcacaga cctcgccgtg
    10981 cacaaaggcg agcgcgatct ggttgcggcc acgcaggggc gttcgttctg gattctcgac
    11041 gatctctccg tgctccatca attgatggac gcgggcggcg cgaaccttgc cagcatggag
    11101 acgcgcctct tcaagcccga agacccgtat cgcattccgg gcggcggtgg cggcgggcaa
    11161 ctacccgcca cggccaccat cggcagcaat ccggcgagcg gcgcggtcgt ctactactat
    11221 ctcaagtcga agccgaagac ggatgttgtg cttgagtttc tcgacgcgac gggcaagacg
    11281 atacgctctt ttacggcgaa agctcccgcg ccgcaaccca gccccgcgcc cggaagcgcg
    11341 gctgtgcaga cgccgcccga acaaccgcag gccgcttcgg gcgaagagtc ctccttcttc
    11401 gcgggcggcg gtggcagcgc gccccgcgtc acgacggaag ttgggctgaa ccgtttcgtc
    11461 tggaacatgc gccacgcgga tgcggtgcgt ttccccggca tgatcttgtg ggctggcagc
    11521 acgacgggtc cacgcgtcat tcccggtcgc tatcaggttc gtctcacggc ggacggcaag
    11581 acctacacgg agactttcga agtgcgcaaa gacccgcgcc tcacgacgac cgacgcagac
    11641 ttcgcgcgcc aactcgactt gcagttgaag attcaagaca agttgaccga gacgcacaac
    11701 gcgatcatct cgatccgcga agcgcgcacg cagatcaacg acctgctgaa acgcgtgcag
    11761 gggcagccga acttcaagcc gatctccgac gccgcgaaat ctctgaacgg gaaaatgacc
    11821 gacatcgagg aggctctcta ccagacgaag aaccagagca gccaagaccc gctcaactat
    11881 ccgattcgcc tcaacaacaa actggccgcg ctcggcggcg tcgtctcaag cgcggacagc
    11941 gcgccgaccg aacagtccta cgccgtctat gaagacctct cggcgaagat caacgcgcag
    12001 ttgcaaaaac tcgatgtcgt gatgaaaagc gacctcccag cctttaacgc actcgtgcgc
    12061 gagcagaaca tccccgcagt gatcttgaag cctacggcgg cgacgccgca gggagtcagg
    12121 ccgtaaaagt cgcatgttca atattgatct gagccttgaa gtcgcggcgg cgatagaggc
    12181 aggccgcccg gtcgtcgcgc ttgaatccac ggttatcgcg cacggtctgc cgcgccctgc
    12241 aaatctcgaa accgcgcgcc gcctcgaaca gatcgtgcgc gcagagggcg caattcccgc
    12301 cactatcgcg atcctcggcg gcaaattctg cgtcggcctg agtgatgagc aactcgcgca
    12361 catcgccgcc agccgcgacg tgaaaaagat tagctgtcgc gatctgccca tcgccgccgc
    12421 gcgcctctgg gacggcgcaa cgaccgtcgc ttcgacgatc tggatcgcag agcgcgcagg
    12481 catcggcgtc ttcgccacgg gcggcatcgg cggcgtgcat cgcggcaacc tgccggacgt
    12541 ttccgcagac ttgccggaac tggcgcgcac gcgtctcgtc gtcgtctgct ccggcgcgaa
    12601 gatcgtgctc gaccttccgg ccacgcgcga gtggctggag acttacggcg tcaccgtcgc
    12661 gggctacggc tgcgacgagt tgcctgcgtt ctacacgcag cggagcggtc tgcacgtgga
    12721 cgtgcgcgtg gacacgccgg aagaggcgtc cgcgatagcg cgtgcgcaga ggcaactggg
    12781 gaaggacggc gcgctgctcg tctgcgtgcc cgtgcccgcc gcgagcgaag tggcagcaga
    12841 cgttctccag gaaactttgg aagcggctct cgccgaagcc gcaggcgcag agatcaacgg
    12901 acgcgaattg acgccgttcc tcctctcccg catgtccgag cgaagcgggg gcgcgacgct
    12961 caaagccaac gtcgcgctgc tcgaaaacaa cgcacgcatc gccgcacaga ttgccggagc
    13021 ggtcgcccgt cagtagccgg ttgcccgttg ccggttgcct ccggctgttg cgctgacggc
    13081 taaagatttg gctcgggcta cggatcgccg gccaccggcg tttttatgat tatggttgaa
    13141 gttgaagaga agaagcgcgc cgcgaagatt atcggtaagc tcaagcgcgc atatccgcag
    13201 gcgcgttgct cgctgaatta ctcgacgccg cttgagttgt tgatcgcgac gatcctttcg
    13261 gcgcagtgta cggacgagcg tgtcaacctc gtgacgaccg acctgttcag gaaataccgg
    13321 ggcgcggatg attacgtgcg cgccgcgccc gccgaacttg agcgcgacat tcattccacg
    13381 ggattttttc gcaacaaggc gaaggcgatt cagggcgcgt gccggatgat caatgaagag
    13441 tatggcgggc gcgtgccgga aacactcgaa gagttgatcg cgcttccggg cgtggcgcgc
    13501 aagacggcca acgtcgtgct cggcaacgct tacggaatcg cgtccggggt cgtcgtcgat
    13561 acgcacgtct cgcgcgtcgc ccaacgtttg aagttgaccg gacacgagca gcccgaaaag
    13621 atcgaacgcg atctcatcga actcgtgccg cgcgctgact ggatcatctt cccgcacctt
    13681 ctcatcgcac acggacgcgc cacctgcaaa gcccgcaccc ctctctgcgc cgaatgcccc
    13741 gtcgaaccgc tctgcccctc atcaatgaaa gggatgaagg atgagggatg aatcagaaca
    13801 gcatccgggt caggcaacct gctttctctt attcatccct catccctcat ccttcatcct
    13861 ttgcttcgat ccagtcgcgc gtgatgtcgc accagagggc gaggccgcgc cattcgcgga
    13921 agcgcgcgta gtggcgtgcg atcttgcggt cgtcggcggt gcgcccttta ttgtggaggc
    13981 gtgcgaaggt ggcgcgcacc catgaatcga gcgcgaggca atcgtagcgt ccgaggagtt
    14041 tcagtagatt gtctgcggcg tattcgccga cgcccttcac gcgcttcatc tccagcttga
    14101 gttcggcggt tgaaagctcg cttgtcaacc aactctctac gtcgagttca ccggaagcga
    14161 cgcgtccggc gagttcggtg aaatagcccg cgcgataacc ggcgcgcacc acgtctcgat
    14221 aaaaactctc cggctggcct gccatcgctt ccggcgtggg aaacgcgcgc ctgccatcct
    14281 gcgcctcacg tccgagatgt ttgaccatcc cttcgaccat cttcatcgtc aaagcccatg
    14341 agcagttggt cgtgcagatg gtcttcacca aatcttcgta gacggtgggc gagcgcagca
    14401 ggcgacccgc gccctcccgt acgatccaac tgaaattcgg atcgtctgcg accgccgcat
    14461 agaaaacctg catctcgtcg tcgagacgaa acatgtggcg cacgtcgcgc gtgatctgtg
    14521 cggtgtcgcg cttgttgagt gtgcgtcgag gaatattgac ttgcagcgcg tcgtccgaag
    14581 aagtgacatg gacggtgacg ggagtggcag cagggagcga gaggacgcgc gtgatgctcc
    14641 acacggtgtc gtcgaactcg aagggcagga gcgaacgcca gccgtgactc ttggctgtgc
    14701 gtttgaagtt gaaggcaggc ggaaccggaa ttgagatttg cacggaagaa ttttcgcgtt
    14761 cgttgaagaa tggtcggagc gcgagagagt ttaacaaatt gaacgggaac cgtcgtcgcc
    14821 gcagggatat gtgtgatcgc ggagggagga atcgaaccgc cgtcgcccga ttatcggtcg
    14881 ggtgctactg ccattgagct actccgcgaa aagaactttt ggcgcacgcg cccggcctcg
    14941 gaaggttcga ccgccagcga cggtcgaacc ttgccgagga taaaaatacg gacgccgtta
    15001 actcttggtg tcgcaagcat ccgtctcatc tttggttgac ccgacgggac tcaaacccgc
    15061 acctcacgga ttaaaagtcc gctgcactgt cgctcgtgct ccgggtcaac aagagttggc
    15121 tgtggtggaa ggactcgaac cttcgatagt cgcattaaca gtgcgatgcc ttgccgcttg
    15181 gctacaccac agtgaaattt ttctcccaag ccgttctggc gggaaggcgc acgggcgcgt
    15241 tcgtgtgatc gcgcccgtgt gcctttgctt ggggtgggga actggagagg ctggaaaatg
    15301 gtttttgcgg gggcaggagt cgaacctgcc gtcgtgcgtg gtttatgagg ccacggagcc
    15361 gcccttgctc tatccccgcg tcaaacgaaa tggctgcggc ggcgagattc gaactcgcat
    15421 caactcgttt acaagacgag cgctctgtgt ccgttgagct acgccgcaaa aaaattgatg
    15481 gaatgggaga gattcgaact ctcacctaac agtttgcaag actgtcgctc tgccaaattg
    15541 agctaccatc ccaaaaccaa acagcgaaag aattgattgc ggaggcggga cttgaacccg
    15601 cgcactttcg cttatgaggc gaatgctctg ccgcctgagc tactccgcaa tatgaaaaat
    15661 atccgaagag aatttggcgg gtctggcagg actcgaacct gcgcggactc gtttagaaga
    15721 cgagcgctct gatccgactg agc
//
LOCUS       DASWNS010000278         7120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_62290, whole genome shotgun sequence.
ACCESSION   DASWNS010000278 DASWNS010000000
VERSION     DASWNS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62290"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VGB76_09920"
     CDS             complement(<1..258)
                     /locus_tag="VGB76_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099253.1"
                     /translation="MDSNTFLVVAIIAIAAQSLLLFLALFEPGLDYKIKQSVFCLPDS
                     EEFMRILEALTDAHVTRRSSIEVFTNGENYYAAELEAIRRAE"
     gene            complement(382..1374)
                     /locus_tag="VGB76_09925"
     CDS             complement(382..1374)
                     /locus_tag="VGB76_09925"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HEY0099254.1"
                     /translation="MTRKMFSVALLMLASLGLNTVSACAAKTETREDAGGSEQSKSRK
                     QTPLPTPMPAPAGEDKLEKNVSSEVRELAAGGYGSVRAPFVFVARDAETYASLRALND
                     KLPELGTDFFKSNAVVAAFLGQRRSGGYGVRIERAAGGLVRISEQTPPKDAMLTMALT
                     APFRIVSVGVREESPLALELDAAWQLAARPYKVSAGEFTRMGGFAGRPERSSLKGDIR
                     IMRHAQLATLFFALQGTGAENSAHALQDTASGSVTPEGGLVLARLDPGSFVPPPRYPL
                     RVRGNFSDNEGTLSLTFEITQAKVNDGYGGQGKLEATATAPPTKKRADGDDQPM"
     gene            1659..1841
                     /locus_tag="VGB76_09930"
     CDS             1659..1841
                     /locus_tag="VGB76_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099255.1"
                     /translation="MSKAEESKTPVICERCDKESAHMSELKEPDNRVHYVCWSCVNRT
                     EKRINISRRWQRARRG"
     gene            2167..2769
                     /locus_tag="VGB76_09935"
     CDS             2167..2769
                     /locus_tag="VGB76_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEY0099256.1"
                     /translation="MKRNFSERGVSALIAILALSVVSFAQSAQASFPNVKIKNFGQMD
                     ERFYRGAQPKENDYRSLAELGVKTVIDLRNDAKDYGRPAAEALGMRYVNIPMSDRHYP
                     REEQIEAFLKLVNDPDTGAFYVHCAGGRHRTGVMGAVYRFTKYGWNYEQVYKEMKDYD
                     FYTRWGHGDMKKYVQDYWQRVQTTPAPTPATALVVAPALK"
     gene            complement(2874..3893)
                     /locus_tag="VGB76_09940"
     CDS             complement(2874..3893)
                     /locus_tag="VGB76_09940"
                     /inference="COORDINATES: protein motif:HMM:NF027640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEY0099257.1"
                     /translation="MRRTNQQKTLIVGVIAALVGFALVALLMSGGRTNAPAPPNSPAA
                     EQAAETTTTQQTAPEQNSPPDAPAAAAEQSAPNDRAGQQAPAASRAQGEGENSIIETL
                     FGADDVSAPNAMPREHWTILVPRIIIRLGLAALLVAALAFRPRRRGVSVLQRNPYVAQ
                     TQILLAVVASALMMIVGDSAARAFGIFAAASLVRFRTNIRDPKEITVLLISLAIGLAT
                     GVGRWELALTLAVFVFPLLWVLEHYAYNQVMRSMQVTVKTRNLLQTQATLISLFRRRG
                     FTAEIRQLEPPTEEDPVGCVVYSLNMNLGVSTDELSEEITAAHPENVQGIEWEQKKNT
                     GYIYQ"
     gene            complement(3983..4954)
                     /locus_tag="VGB76_09945"
     CDS             complement(3983..4954)
                     /locus_tag="VGB76_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099258.1"
                     /translation="MFRQNRFRAFMIMLIILALGLSTNGFAVQKKTKKKKPMPAGTPV
                     MWREPADIATRDLFHGSGGEAGKPDLSRVTLVEKVEGGFSEKYRVRDAAGREWVAKVS
                     REAQSETAAVRLLWAVGYMTELTYLAPTVTIAGTPKGTYENVRFEGRSKEVKRLDPWL
                     WDKNPFSGKRELQGLKVMMLLLNNWDIKDDNNGILHVVTPEGQSELHYIVSDLGATFG
                     KIGSGPLWALKRSRNNPEDYAESKFVDHVHKNLVYFHYGGKKQDIFEDISVNDVQWIA
                     GLLSRLSDQQISDAFRAANYSAEEVTLLTTAFRSRINELVNLPPAES"
     gene            complement(4996..>7120)
                     /gene="ppk1"
                     /locus_tag="VGB76_09950"
     CDS             complement(4996..>7120)
                     /gene="ppk1"
                     /locus_tag="VGB76_09950"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY0099259.1"
                     /translation="FPEDRRGDASGAGTVPPKLMDARQLLFNREFSLLEFFRRVLEEG
                     LDETQPLLERLKFLAIFSSNVDEFFMIRVSGLKEEFEHDLTETSPDGMLASDQLAEIR
                     ARLLPMLEEQSRCLLEELLPALKAQGIVVASYGPLSEEERATLQEYFDEQVFPVLTPQ
                     AVDPSHPFPYISNLSLNLGLMVAPLPEHGITSSLVGKIEPRFARIKVPPLVPRLIPIG
                     KDNSKFILLEDLIAANVRELFPRMRATACHAFRVTRDADIDIREDEADDLLRIMQQTL
                     RKRRFGTPVRLEVATTMPPEMVQYLSKSIGVEAEDVYRSSCPLAVTDLMRLYKLERPD
                     LKDAPFQQRVPATLRRPHLSIFDAIREQDILLHHPYTSFSPVADFINAAARDEQVAAI
                     KVCLYRTGPNSPIPQALIEASEQGKQVTAIIELKARFDEENNIEWAQRLEEVGVHVVY
                     GLMGLKTHCKLALVVRREDNTMRRYVHIATGNYNPDTSRGYTDLGLFTADEEIGADAS
                     ELFNYLTGYSRQKEYRRLMVAPVNLRESMLALIRREAEHAKAGRTARMVIKINRLADV
                     EIIRALYEASQAGVRIDLIVRGICMLRPGVENLSETITVRSIVGRFLEHSRVFYFANG
                     GDEELYTGSSDWMPRNLNRRVEVVTPVHDARLRTHLKDVILAAYLRDNVKARLLRPDG
                     SYERIQRAPGEERFDSQLHFMNVEL"
BASE COUNT         1371 a         2076 c         2195 g         1478 t
ORIGIN      
        1 ttcggcgcgg cggatggctt cgagttcggc tgcgtagtag ttctcgccgt tggtgaagac
       61 ttcaatcgaa ctgcggcgcg taacgtgcgc atcggtgagc gcttcgagga tgcgcatgaa
      121 ctcttcggag tcgggcaggc aaaaaacgga ctgcttgatc ttgtaatcca agcccggctc
      181 gaagagcgcg aggaacaaaa gcagactctg cgcggcaatg gcgatgatgg cgacgacgag
      241 aaaggtgttg gaatccactt ggtcagttaa aaagtctcag gccgagaatt tcaaactcga
      301 atttgcaggg gaaagccttc gcgttcagtc tagcacgact gaggcgtttg cggcctcgct
      361 taaacaggcc accgtgcccg attacatcgg ctggtcgtcg ccgtcagcgc gcttcttcgt
      421 cggcggggcg gtggccgtcg cctcaagttt tccctgtccg ccgtagccgt cgttgacctt
      481 cgcctgtgtg atctcaaacg tgagcgatag cgtgccttcg ttgtcgctga agtttccgcg
      541 cacgcgcaga gggtagcgcg gcggcgggac gaacgaaccg gggtcgaggc gcgcgagcac
      601 gaggccgcct tcgggcgtga cgctgccgct cgcagtgtct tgcagtgcgt gcgcgctatt
      661 ttccgcgccc gtgccctgca aggcgaagaa gagcgttgcg agttgagcgt ggcgcatgat
      721 gcgtatgtcg cccttgagcg acgaacgctc cgggcgaccg gcaaagcctc ccatccgcgt
      781 gaactcgccc gcgctcactt tgtaggggcg cgcggccaac tgccacgccg catcgagttc
      841 gagcgcgagc ggactctctt cccgcacgcc gaccgagacg atgcggaacg gagcggtcaa
      901 agccatcgtg agcattgcgt ctttcggcgg agtctgctcg gagatgcgca cgaggccgcc
      961 cgccgcgcgt tcgatgcgga cgccgtaacc gccgcttcgc ctttgaccga ggaaggccgc
     1021 gaccactgcg ttcgacttga agaagtccgt gccgagttcc ggcagtttgt cgttcaacgc
     1081 tctgaggctc gcgtaagtct ccgcatcgcg tgcgacgaag acgaaaggcg ctctgaccga
     1141 gccatagccg ccagccgcca actctctgac ttcgctcgaa acattcttct ccaacttgtc
     1201 ctccccggca ggcgcaggca tgggcgtcgg cagcggcgtc tgctttctgc ttttcgactg
     1261 ttcgcttccg cccgcgtcct cgcgcgtctc tgtctttgcg gcgcacgcgc tcacagtgtt
     1321 gaggccgagc gacgcgagca tcaacagggc gacgctgaac atttttcgag tcattttcgt
     1381 tcgcactccg ttttccatat ttcgccagcg tataaagtga aaagcgggag attcgagttg
     1441 caggcacgcg ggagaggacg cgtcggggca ggccgaaatt tgcccgcgca cttgggattc
     1501 gattcgtgcc ctcaagtttg cacgtctgat tctaacaaaa cgttccggta ttttgttgca
     1561 cagtcttctt cgagactgaa catgcgggct tttttcagtt aaactgtcca cgaaacgaca
     1621 aacgagacgg cggcgggacg aggacgggag cgaaagtgat gtctaaagca gaagagtcga
     1681 aaacgcctgt gatttgcgaa cgctgcgaca aagagagcgc gcacatgtct gaattgaagg
     1741 agccggataa ccgggttcat tacgtctgct ggtcgtgcgt caatcgcacg gaaaagcgca
     1801 tcaacatcag ccgtcgttgg cagcgtgcgc ggcgcggctg attgcacgcg cccgccgcgc
     1861 ctgaaaactc cgcccgcctg cttctcttaa acatcctcga tgtggtaatt ttaggcgtct
     1921 cgtggagcca gggttcgttc acttttcaaa gacgaaacgg acaacgagaa aagctccggg
     1981 gctatccaac ttccggtcgc gcacgttctg cgaaagattg aaaaatccgt ctgcccgcgc
     2041 cgtcttgaag tagtaaagtc tggccgtggc aatcgttcag accggaagcg tgatagcccc
     2101 accagaccaa cctctcaaac ttgatagttt tgtttctttc aaatttagga gaatttcttg
     2161 atgcgtatga agcgtaattt ctcagaacgc ggcgtcagtg ctttaatagc cattctggct
     2221 ttgtcggtcg tctccttcgc gcaatccgcg caagcatcct ttcccaacgt taagatcaaa
     2281 aatttcgggc agatggacga gcgtttttat cgcggcgcgc agccgaagga gaacgactac
     2341 cggagcctcg ccgaactcgg cgtcaagacc gttatcgacc tgcgcaacga cgcgaaggat
     2401 tacgggcgac ccgccgcgga agccctcggc atgcgctacg tcaatatccc gatgagcgac
     2461 agacactatc cgcgcgagga gcagatcgag gcgtttttga aactcgtgaa cgaccccgac
     2521 accggcgcgt tttacgtcca ctgcgcgggc gggcgtcatc gcacgggcgt gatgggcgcg
     2581 gtctatcgct tcacgaaata cggttggaat tacgagcagg tttataaaga gatgaaagac
     2641 tacgacttct acacgcgctg gggacatggc gacatgaaga agtatgtgca ggattactgg
     2701 cagcgcgtcc agaccacgcc tgcgccgacg ccagcaaccg cactcgtcgt agcacccgct
     2761 ctgaaatagt ttagacgccg gttcatctga aaaaacgaga aggccgcttc ccacttggaa
     2821 gcggccttct cgttttttcg cgccagtctc ttctcgtcta tgagccggga acgctactgg
     2881 taaatgtagc ccgtgttctt cttctgttcc cactcgatcc cctgaacgtt ctccggatgg
     2941 gcggcggtga tctcttcgct caactcgtcc gtgctcacgc ccagattcat gttcaacgaa
     3001 tagacgacgc aaccgaccgg gtcttcttcc gtcggcggtt cgagttggcg aatctccgcc
     3061 gtgaagccgc gtcggcggaa gaggctgatg agcgtcgcct gcgtctgcaa caggttgcgc
     3121 gtcttgaccg tcacctgcat cgagcgcatg acctgattgt aagcgtagtg ttcgagcacc
     3181 cagagcagcg ggaagacaaa gacggcgagc gttaaagcca actcccaacg cccgacgccc
     3241 gttgccagac caatcgcgag gctgatcagg agcactgtga tctctttcgg gtcgcggatg
     3301 ttcgtgcgga agcggacgag cgaagcggcg gcgaagatgc cgaaggcgcg cgccgcgctg
     3361 tcgccgacga tcatcatcag cgcggaggcg acgacggcca gcagaatttg cgtctgcgct
     3421 acgtaaggat tgcgttgcag gacggacacg ccacggcgtc gcgggcgaaa ggccagcgcg
     3481 gcgacgagca gcgcggcgag tccgagccgg atgatgatgc gcgggacgag aatcgtccaa
     3541 tgctcgcgcg gcatggcgtt gggcgcgctc acatcgtctg cgccgaagag agtctcgatg
     3601 atcgagtttt cgccttcgcc ctgtgcgcgt gaggccgcag gagcttgctg cccggctctg
     3661 tcgttcggcg cggactgttc ggcggcagcg gcgggcgcgt ccggcggcga gttttgctcc
     3721 ggggctgtct gttgtgttgt ggttgtctcg gcggcctgtt cggcggcggg cgagttcggc
     3781 ggcgcgggtg cgttcgttct gccgccggac atcaagagag cgacaagcgc gaatcctacg
     3841 agcgcggcga tcacgccgac gatcaaagtt ttctgctggt tggttctgcg catttcacct
     3901 taactatact acgcccgccg tttgatagcg acgggcgtgt gcaaaagaga gtatccgaaa
     3961 gacgtggact acgagacgcg ttctaagatt cagcgggcgg gagattgacg agttcgttga
     4021 ttctcgaacg aaacgcggtg gtcaggagcg tcacctcctc cgcgctgtag ttggccgcac
     4081 ggaaggcatc gctgatctgt tggtcgctca ggcgcgagag cagccccgcg atccactgca
     4141 cgtcgttaac cgagatgtct tcgaagatgt cctgtttctt gccgccatag tgaaagtaga
     4201 ccagattctt gtggacgtgg tccacgaact tcgactcggc gtagtcttcc ggattattgc
     4261 ggctgcgctt caaagcccag agcggcccgc tgccgatctt gccgaaggtc gcgccgaggt
     4321 cgctgacgat gtagtggagt tcgctctgtc cttccggggt gacgacgtgg agaatgccgt
     4381 tgttgtcgtc tttgatgtcc cagttgttca gcaggagcat catcactttc aagccttgca
     4441 gttcacgctt gccggagaaa gggtttttat cccacagcca cgggtcgagc cgcttcacct
     4501 ctttcgaacg tccctcgaac ctcacatttt cgtaagtccc tttcggcgtg cccgcgatgg
     4561 tcacggtcgg cgcaaggtaa gtaagctccg tcatgtagcc taccgcccac agcaagcgca
     4621 cggcggctgt ctccgactgc gcctcgcggc tgactttcgc cacccactcg cgccccgccg
     4681 cgtcgcgcac gcgatacttc tccgaaaagc cgccctccac cttctcgacg agggtgacgc
     4741 gcgacaggtc gggtttcccg gcctcgccgc ccgaaccgtg aaacagatcg cgcgtggcga
     4801 tgtccgcagg ctcgcgccac atgacgggcg tgcccgcagg catcggcttc ttcttctttg
     4861 tttttttctg aacggcgaac ccgttcgtcg aaagaccaag cgcgagaatg atcagcatga
     4921 tcataaaagc gcgaaatcta ttttgtcgaa acatgcaact agactcctcc ggcagtttga
     4981 acttgatcgc cgagcttata attcgacgtt cataaaatgt agttggctgt cgaatctctc
     5041 ctcacccggg gcgcgttgga tgcgctcgta agagccatcg ggacgcagca ggcgagcctt
     5101 cacgttgtcg cgcaaataag cggctagaat cacgtccttc aaatgagttc gcaggcgcgc
     5161 gtcgtgcacg ggcgtgacga cttcgacgcg ccggttgaga ttgcgcggca tccaatcgga
     5221 actgcccgtg tagagttctt cgtcgccgcc gttggcgaaa tagaagacgc ggctgtgttc
     5281 gaggaagcga ccgacgatgc tccgcacagt gatcgtctcg gagaggtttt cgacgccggg
     5341 gcgcagcatg cagatgccgc gcacgatgag gtctatccgc acgcccgcct gcgacgcttc
     5401 atagagcgcg cgaataatct ccacatcggc cagacggttg atcttgatga ccatgcgcgc
     5461 cgtacgaccc gccttcgcgt gctccgcttc gcgccggatg agcgcgagca tgctctcgcg
     5521 caggttaacg ggcgcgacca tcaagcgacg atactctttc tgccgcgaat agccggtcag
     5581 gtagttgaag agttcggagg cgtccgcgcc gatctcttcg tcggcggtaa agagtccgag
     5641 gtctgtgtag ccgcgcgagg tgtcggggtt gtaattgcct gtggcgatgt gtacgtagcg
     5701 gcgcatcgtg ttgtcttcac ggcgcacgac gagtgcgagc ttgcaatgcg tcttgagtcc
     5761 catcaaaccg taaacgacgt ggacgccgac ctcttcgagc ctctgcgccc attcgatgtt
     5821 gttctcttca tcgaagcgcg ccttgagttc gatgatggcc gtcacctgct tgccctgctc
     5881 gctcgcttcg atgagcgcct gcggaatggg cgagttcggg ccggtgcgat agagacagac
     5941 cttgatggcc gccacttgct catcgcgcgc cgccgcgttg atgaaatccg cgacgggcga
     6001 gaaggaggtg taaggatgat gcaggaggat gtcctgctct ctgatcgcgt cgaagatcga
     6061 taggtgtgga cgccggagcg tggcgggcac gcgctgctgg aagggcgcat ccttcaagtc
     6121 cggcctctcc agtttgtaga gccgcatcag gtctgtgacg gcgagcgggc aactgctccg
     6181 gtacacgtct tccgcctcga ccccgattga cttcgacaaa tactgcacca tctccggcgg
     6241 catcgtcgtc gccacttcaa gcctcacggg cgtgccgaag cgacgcttgc gcagcgtctg
     6301 ttgcatgatg cgcaaaagat cgtccgcttc gtcttcgcgg atgtctatgt cggcatcgcg
     6361 cgtgacgcgg aaggcgtggc acgcggtggc acgcatgcgg ggaaacagtt cgcgcacgtt
     6421 ggccgcgatc aaatcttcga gcaggataaa cttcgagttg tctttgccga tagggatcag
     6481 gcgcggcacg agcggcggca ctttgatgcg cgcgaaacgc ggctcgatct tgccgacgag
     6541 cgaactcgtg atgccgtgtt cgggcagcgg cgcgaccatc aggccgaggt tgaggctcag
     6601 attcgagatg tagggaaacg ggtggctcgg gtcaaccgcc tgcggcgtca gcaccggaaa
     6661 gacctgttcg tcgaagtatt cctggagcgt ggcgcgttcc tcttcggaca gaggaccata
     6721 ggacgcaacg acgatcccct gcgccttgag cgcgggcagc aactcttcga gcaggcagcg
     6781 actctgttct tcgagcatcg gcagcagtcg cgctctgatc tcggcgagtt ggtcggaggc
     6841 caacatgccg tcgggcgaag tctcggtcag gtcgtgctcg aactcctctt tgagtccaga
     6901 cacgcggatc atgaaaaact cgtccacgtt cgaagagaag atggcgagaa acttgaggcg
     6961 ttccaggagc ggttgcgttt cgtccaagcc ctcttcgagc acgcgccgga agaactccaa
     7021 caagctgaac tcgcggttga agagaagttg ccgtgcgtcc atcaacttcg gcggcacggt
     7081 tcctgcgccg gaagcgtcac cgcgacggtc ttcgggaaag
//
LOCUS       DASWNS010000279         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_399971, whole genome shotgun sequence.
ACCESSION   DASWNS010000279 DASWNS010000000
VERSION     DASWNS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399971"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VGB76_09955"
     CDS             complement(<1..1157)
                     /locus_tag="VGB76_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099260.1"
                     /translation="MALFLFTSTLFLAFPLPHLADSKYSTLLSESLIHRRSFTLDAYK
                     IPDLVPAQTYNSASNTTVYQIEFSGGHFYHMFPPGSPVLSVPYVALFNLFGITASNPD
                     GSYNNKNEKIIQASLASLLMGALTVIFFYTSRQLLPPAWSALIALGGSLGTQVWSTAS
                     RVLWSDTWGIFLLGYVVLLLVSDATGRRPLRPVWFATLLSWMYFVRPTYSIPIAAISI
                     YVFLYRRRLFASYALTGAAWFAAFVLYSQLHFGQMLPYYYRATRLKFDSFPTAFAGNL
                     ISPARGLFVYVPVLFFVAYLLLRFERELPLPRLAVLALGVIVAHLFVVAGFTPWWGGH
                     SYGPRYTTGLVPWFVLLGILGIKAMLGWRERHRNVAGTMRERVPLFIGGALL"
     gene            1478..2128
                     /locus_tag="VGB76_09960"
     CDS             1478..2128
                     /locus_tag="VGB76_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099261.1"
                     /translation="MGKRLTTSLCVLALSILAAGCGGSDNTNAGNSNAANANANMTSG
                     MTNMTPTPGGTAAADGEVVTTEANGVKTQTRTFNNNPRVQRVVVTTDASGRRTGRVYS
                     STGDVRELPEGKIDQALTATGDAVADAAGVVGDKAADIGRGAADKAEDVGRGAADKAE
                     DVGRGAADKAEDIGRGAADKAEDVGRGAVKGAKKVGEKTADGAKKVGGAIKDAVKP"
     gene            2359..>2650
                     /locus_tag="VGB76_09965"
     CDS             2359..>2650
                     /locus_tag="VGB76_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099262.1"
                     /translation="MTDKARYTLICPCCESTLIIDAQTGALISHEEKAKPIASFEEMA
                     KGMERQKQTRDQIFAQELGSLKDRERILEEKFREALKRADKDKDKPFRNPLDI"
BASE COUNT          657 a          697 c          875 g          421 t
ORIGIN      
        1 agcaacgctc cgccgatgaa gagcggcacg cgctcgcgca tagttcccgc cacgtttcta
       61 tgcctctctc tccatccgag catcgctttg ataccgagta tgccgagcag cacaaaccaa
      121 gggacgagtc cggtcgtgta acgtggcccg taggagtggc cgccccacca gggcgtgaac
      181 cctgcaacca cgaagaggtg cgcgacgatg acgccgagcg cgagcactgc gagacgcggc
      241 agcggaagct cgcgctcgaa acgcagcagc aggtaagcga cgaagaagag cacagggaca
      301 tagacgaaca gcccgcgcgc ggggctgatg agattgcccg caaacgctgt ggggaacgag
      361 tcgaatttga gacgagtggc gcggtagtaa tagggcaaca tctggccgaa gtgaagttgg
      421 gaatagagga cgaaggccgc gaaccatgcc gcgcccgtga gggcgtagga ggcgaagagt
      481 cgtcgccggt agaggaacac gtaaatactg atggctgcaa tcgggatgga gtaagtcgga
      541 cgcacgaagt acatccagga gagcagggtc gcaaaccaga cggggcgcaa gggtcgtctg
      601 cctgtggcgt cggagacgag cagcaggacg acgtagccga gcagaaaaat tccccacgtg
      661 tcagaccata agactctgga agctgtgctc cacacctgcg tccccagaga gccgccgagg
      721 gcgatgagtg cgctccaagc gggcggcagt agttgacggc tcgtgtaaaa gaagatgact
      781 gtgagcgcgc ccatcaacag cgaggcgagg ctcgcctgaa tgatcttttc gttcttgttg
      841 ttataacttc cgtcggggtt agaggcggtg atgccgaaga ggttgaacag cgcgacgtag
      901 ggcacggaga ggacggggct gcccggcgga aacatgtgat agaagtgccc gccggaaaac
      961 tctatctggt agacggtcgt gttcgaggcc gagttgtagg tttgcgccgg aacgaggtcg
     1021 ggaattttgt aggcgtcgag ggtgaaactg cgccggtgaa tcaggctctc gctcaggagc
     1081 gtggaatatt ttgaatccgc gagatgcgga agcgggaagg cgagaaaaag ggtgctggtg
     1141 aagaggaaga gagccacgcc tatcgctctg tctaagttag tgccgtcaag cctccagcgc
     1201 ggcgcagccc gcaaggcatc acgcgtcaaa tgcacacctc cgcacgcatc aatcgtctaa
     1261 aaacagagat tgatcttaag tgacgaactc tgcaatctca agtatggata aaggacgcgc
     1321 tgcatctgca tcgtacacag cgagaacgca gggcagggaa tggagccgct tttgccagca
     1381 aaccgtgcct gcggagggag atcaggcatg gcacgtcggt tgcgcttccg ctcaggcaga
     1441 taaagataat tctgcttaaa acggaggaaa gataaaagtg ggaaaacgtt taactacaag
     1501 cctgtgtgtg ctggctttgt caattttggc cgccgggtgc ggcggtagtg ataacactaa
     1561 tgcgggtaac agcaacgcgg cgaacgccaa cgccaacatg acttccggaa tgaccaacat
     1621 gacgccgacg ccgggcggca cagccgcagc cgatggcgag gtcgtgacca ccgaagcgaa
     1681 cggcgtgaag acgcagacgc gcactttcaa caataacccg cgcgtccagc gcgttgtcgt
     1741 cacgaccgac gccagcggca ggcgcacggg gcgtgtctac tcaagcaccg gcgatgtccg
     1801 cgaactgccg gagggtaaaa ttgatcaggc tttgacggcg acgggtgatg cagttgccga
     1861 cgccgccgga gtggtgggcg ataaggccgc agacatcggc agaggcgcag cggacaaagc
     1921 cgaggatgtg ggcagaggcg cggcggataa ggccgaggat gttggccggg gcgcagccga
     1981 caaagcggag gacatcgggc gcggcgcggc tgacaaggcc gaagatgtcg ggcgcggtgc
     2041 ggtaaagggc gcaaagaaag tcggcgagaa gacggctgac ggcgcgaaga aggtgggcgg
     2101 cgcgatcaaa gacgccgtga agccgtaagc tgacgacggg caaaaattag tcgaaattag
     2161 tcgaagggga aagccgcaga cgaggccagc cggtgctgtt cgtctgcggc tttcatttct
     2221 ccgtttaagg cgcgttgtca tcctgcaatt tacgcgcgtg tgcatgcgtc gcttcgaccg
     2281 cgtgaagtgt tataatttgc cacgcaaacg ttccggcgaa agcgcgcatg actatattca
     2341 gacgaaggag tttcggttgt gacggataaa gcacggtaca cactcatctg cccctgctgc
     2401 gagtccaccc tcatcataga tgcgcaaacc ggcgcgctca tctctcacga agagaaggcg
     2461 aagcccatcg cctcgtttga agagatggca aagggcatgg agcgacagaa gcagacgcgc
     2521 gaccaaatct tcgcgcagga actcggctca cttaaagacc gcgaacgcat cctcgaagag
     2581 aagttccgcg aagccctcaa gcgcgccgac aaagacaagg acaagccctt ccggaacccg
     2641 ctcgacatcg
//
LOCUS       DASWNS010000280         4047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_89072, whole genome shotgun sequence.
ACCESSION   DASWNS010000280 DASWNS010000000
VERSION     DASWNS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89072"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..3936)
                     /locus_tag="VGB76_09970"
     CDS             complement(157..3936)
                     /locus_tag="VGB76_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0099263.1"
                     /translation="MRAFPVRLCSTAIRFVFVCLFAAALAGAAFAQAQSNASDLQGYL
                     RDAQGGLVVNAAVTARNKATGLSRTAQSNDEGFYQITNLPPGEYEVTVEAANFSRAQI
                     PSVTLTVGQRADLDIPLAAGQVSETVTITGATTEIVETSRTAVTNTIERTRIENLPVN
                     QRDYLGISTTISTVNRGNDRPIGPAPSSGLNIGGQRGRSTLVQVDGADNTDNSINASR
                     STVSQEAVQEFQVATNSYAPEFGRATGGIVNVVTKSGSNEFRGNVFGFLRHKSFQARN
                     AFAPIEDPPFTRAQYGATFGGPIKSERTFFFTAFEQRRRQESAFFSSDVVGNMTSSLS
                     VGPGTAFPFVPLPNTFTNLTPAQAAFITTQLNTGASTGNAALIGAALTYATFASSGGQ
                     IGLNGASTLRSIGGAIPAGQTIGGRFFISGAPVPTAANNGVTAGFTTVNGEGQPIGFR
                     PLNSLARIFPISEATSFFSVRADHQINQSNQLTMRFGYNPSDLTGVQDESQNQVLGQN
                     DFSRTGRQEIRDTSFVTSLTSTLSSRVVNEARFNFGHRDAKFDSQIPGAAIQISGVSF
                     IGSNPFSPVDRTERRFQLTDNVNWLPGNHNFKFGADINFVDVEAVFELNFPGLFNFGE
                     VSGGTLAAGFIPGLPATAPALTPVQAYGAGIPGVFIQGFGNPNSTLKNKPLAFFAQDS
                     WKARPNLTLNYGVRYDVELTETIPTIGFTDPLTGINLSAADLNAAQNVLGVQQGFPRD
                     TNNFAPRVGLAWDITNDGKTVFRAAYGLFYDHPLLAVAFNSDIADAAQQQQFTTVLPG
                     SPAPNASLNLLQIFQGTVVPGVTPGVAASTQYLRGQLRFNDQTFPGFGPVLPFTLAVG
                     QDFEYAYANQANVTLERQLTPNTSVSASYIFVGAHHLPHPRDVNAPQTELLVENFRRF
                     TVGNPVLCGANAACLASGRAPTSFGEAVLFPIPTASNALFTVRIPGLVAVNNVSGQTI
                     VNPLAANFFRPNAPNYFFVQSATGGAVTPAIFNAAINGSVRTPGLISPFGDVSAQLSE
                     GNSNYNAMTLEAKRRFSNNFQFLASYTWSHSIDDSSDLQTLLKPQDNRNFAAERSDSL
                     FDQRHRFVFSGVATSPDSWRNSESGVRRFLSNFTIAPILELSSGRPFNIITGSDANAD
                     LQSSNDRPSVAANGTLFVPPFLSTGNLRRNAGITHGYASLDLRISRAIRFSETTRLDI
                     IAEGFNLFNRFNEAAANPFFDVVNAHNERRGGLYYSRPTAAYDPRQFQFGLKFSF"
BASE COUNT          733 a         1069 c         1507 g          738 t
ORIGIN      
        1 aggccatcgg catctgaccc tgctcacgtg cgtcggcttc cctgcatcga aatcgtgcta
       61 aagccgggac actaaccgga cgccgctcga agcccaccaa gaaagggcac gtcaactcgt
      121 ctgagttgac gtgccctttt cacttgttcg cccgatctag aagctgaact tcaggccgaa
      181 ctggaattgg cgcgggtcgt aagcggcggt cgggcggctg taatagaggc cgccacggcg
      241 ttcgttgtgt gcgttcacca cgtcgaagaa ggggttggcg gcggcttcgt tgaaacgatt
      301 gaagaggttg aagccttcgg cgatgatgtc gagccgtgtg gtttccgaga agcggatggc
      361 gcgcgagatg cgcaggtcga gcgaggcgta gccgtgcgtg atgcccgcgt tgcggcgcag
      421 attgccggtg gagaggaagg gcggcacgaa gagcgtgccg ttggcggcga cgctcgggcg
      481 gtcgttcgag ctttgcaggt cggcgttggc gtcggagccg gtgatgatgt tgaacggacg
      541 ccctgacgaa agctcaagga tcggggcgat ggtgaagttg gacaagaaac ggcgcacgcc
      601 actctccgag ttgcgccaac tgtcgggcga ggtggcgacg ccgctgaaga cgaaacggtg
      661 gcgttggtcg aagagcgaat cggaacgctc ggcggcgaag ttgcggttgt cctgcggctt
      721 caagagggtc tgcaaatcgg atgaatcgtc aatcgagtgc gaccaggtgt aggaggcgag
      781 gaactggaag ttgttcgaga agcgacgctt ggcttccagc gtcatcgcgt tgtagttgga
      841 gttgccttcc gagagttgcg cgctcacgtc gccgaagggc gagatgaggc cgggcgtacg
      901 caccgaacca ttgatggccg cgttgaagat ggcgggcgtg accgcgccgc ccgtcgctga
      961 ctggacgaaa aagtagttcg gcgcgttagg ccggaagaag tttgccgcga gcgggttgac
     1021 gatggtttgg ccggagacgt tgttgacggc gacgagtccc ggaatgcgga cggtaaagag
     1081 cgcgtttgac gccgtaggga tcgggaacaa gaccgcttcg ccgaagctcg tgggcgcgcg
     1141 accgctggcg agacaagccg cattagcccc gcagagcacc ggattgccga cggtgaagcg
     1201 gcggaagttt tccaccagca gttcggtctg cggagcgttc acgtcgcgcg ggtgcgggag
     1261 gtggtgcgcg ccgacgaaga tgtagctggc ggagacggaa gtgttcggcg tcaactgccg
     1321 ttcaagcgtg acgttggcct gattggcgta agcgtattcg aagtcctgac cgacggcgag
     1381 cgtgaagggc aggacgggac cgaagccggg gaacgtctga tcgttgaagc gcagttgccc
     1441 gcgcaaatat tgggtgctcg cggcgacgcc gggcgtgacg ccggggacga ccgtgccctg
     1501 aaaaatctgg agcaggttga gcgacgcgtt cggcgcgggg ctgccgggca ggacggttgt
     1561 gaactgctgt tgctgcgcag cgtcggcgat gtcggagttg aaggcgacgg ctaagagcgg
     1621 gtggtcgtag aagaggccgt aggcggcgcg gaagaccgtc ttgccgtcgt tcgtgatgtc
     1681 ccacgccaga ccgacgcggg gcgcgaagtt gttcgtgtcg cgcgggaacc cctgctggac
     1741 gccgaggacg ttctgcgccg cgttcaagtc tgcggcggag aggttgatgc cggtcagcgg
     1801 atcggtgaaa ccgatggtgg ggatcgtctc cgtgagttcg acatcgtagc gcacgccgta
     1861 gttgagcgtc aggttgggtc tggccttcca ggaatcctgc gcgaagaaag cgagcggctt
     1921 gttcttcagg gtgctgttcg gattgccgaa gccctgaatg aagacgccgg ggatgcccgc
     1981 gccgtaagcc tgcacggggg tgagcgcggg cgcggtggcc gggaggccgg gtatgaaccc
     2041 tgcggccagc gtgccgccgg agacttcgcc gaaattgaag aggccgggga agttcaactc
     2101 gaagacggct tccacgtcaa cgaagttgat gtccgcgccg aacttgaagt tgtgattgcc
     2161 gggcagccag ttgacgttgt cggttaactg gaaacgacgc tccgtgcggt cgacgggcga
     2221 gaagggattc gaaccgatga aggagacgcc ggaaatctgg atggcggctc ccggtatctg
     2281 cgaatcaaac ttggcgtcgc ggtggccgaa gttgaagcgc gcctcgttga cgacgcggct
     2341 cgacaaggtc gaagtcaggg aggtgacgaa ggacgtgtca cggatctcct gccgacccgt
     2401 gcgcgagaag tcgttctgtc ccaagacctg attttgcgat tcatcctgca cgcccgtcag
     2461 gtcgctcgga ttgtagccga agcgcatggt cagttgattc gactggttga tctggtggtc
     2521 tgcgcggacg gagaagaagc tcgtggcctc cgagatgggg aagatgcgcg cgaggctgtt
     2581 gagcgggcgg aagccgatgg gctggccttc gccgttgacg gtcgtgaagc ctgccgtcac
     2641 gccgttgttg gctgcggtcg ggacgggcgc gccggagata aagaagcgtc cgccgatggt
     2701 ctgacccgca gggatcgcgc cgccgatgct gcgcagagtg ctcgcgccgt tcaggccgat
     2761 ctgcccgccg gaggaagcga aggtggcata agtcagggct gcgccgatga gcgcggcgtt
     2821 gccggtggac gcgcccgtgt tgagctgggt ggtgatgaag gcggcctgtg cgggcgtcag
     2881 gttggtgaaa gtgttcggca ggggcacgaa cgggaaggcc gtgccggggc cgaccgagag
     2941 gctcgaagtc atgttgccca cgacatccga actgaagaag gccgactcct gacgacggcg
     3001 ttgctcgaag gccgtgaaga agaaggtgcg ctcggacttg atcgggccgc cgaacgttgc
     3061 gccgtactgc gcacgcgtaa agggcgggtc ttcgatgggc gcgaaggcgt tgcgcgcctg
     3121 aaaactctta tggcgcagga agccgaagac gttgccgcga aactcgttgg agccgctctt
     3181 ggtgacgacg ttgacgatgc cgcccgtggc gcgtccgaac tcgggcgcgt acgagttggt
     3241 cgccacttga aattcctgca cggcctcctg cgagacggtg ctgcgcgagg cgttgatgga
     3301 gttgtccgtg ttgtccgcgc cgtcaacctg tacgagcgtg gagcgtccgc gctggccgcc
     3361 gatgttcagg ccggaagagg gcgcgggacc aatcgggcgg tcgttgccgc gattgacggt
     3421 cgagatggtg gtggagatgc cgagatagtc tctctggttg acgggcagat tctcgatgcg
     3481 cgtgcgctcg atggtgttgg tgacggcggt gcgcgacgtt tccacgatct cggtggttgc
     3541 gcccgtgatg gtcacggttt cgctgacctg tccggcggcg agcgggatgt cgaggtctgc
     3601 gcgttgtccg accgtgagcg tgaccgaagg aatctgggcg cgcgagaagt tcgcggcctc
     3661 caccgtcact tcatactcgc ccggcggcaa gttggtgatc tggtagaagc cttcgtcgtt
     3721 cgactgcgcg gtgcgcgaca ggccggtcgc cttgttgcgc gcggtgaccg ccgcattaac
     3781 gacgaggccg ccctgtgcgt cgcgcaagta gccttgcagg tcggaggcgt tggactgcgc
     3841 ctgcgcgaag gccgcgcccg caagagctgc cgcaaacagg cagacgaaga cgaagcggat
     3901 ggcagtcgag cacagccgca cggggaatgc acgcatgttt atattctcct caaacgtaaa
     3961 ttgtagcttt cagcaagttc agcgggagac gctaaggtcg tttgccgtcg gacaacggga
     4021 cgcgaaccgg ctttgatcaa agcacgg
//
LOCUS       DASWNS010000281         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_321271, whole genome shotgun sequence.
ACCESSION   DASWNS010000281 DASWNS010000000
VERSION     DASWNS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321271"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VGB76_09975"
     CDS             complement(<1..418)
                     /locus_tag="VGB76_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099264.1"
                     /translation="MSAAQPHLSPLFTEAFRQFERNQTPPEIEVRFYPYAGLNHTIRR
                     RSGRIYVRLSDIFRDAPLSVQRALAFMLVASLLRRKTPQVHEHVYRDYAFSPEVQRAS
                     DIARRRRGRKMISTSRGSYHNLNRLFARLNRRYFDDE"
     gene            998..2119
                     /gene="hrcA"
                     /locus_tag="VGB76_09980"
     CDS             998..2119
                     /gene="hrcA"
                     /locus_tag="VGB76_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY0099265.1"
                     /translation="MANRSSTSTTTSERLPDARSQCVLSAIIKEHLGTGEPVGSRTVS
                     ERLAGAAGWSAATIRNVMAELEEAGLVEQPHTSAGRLPTDKGYRYYVDHMLGDARLSR
                     ADTAAIDSLLGLRQGEGSMTAPNRLMEKVSHLLSELSENVGIVISPPMADNRLQHIEF
                     VQLTDGRILVVLVFAPNIIQNKIIRIDEPFTQEELEQTARYLNVEFSGKNLQTIRAEI
                     LGLIRAEKALYDRLLQNAMLLCELSLEGEEAATGDVYVDGASNILVKPDFADIERLRE
                     LFRTLEEKTRLVKILNECLTRERVFGDVRVQIGRELKTPSMQNCALITAPYRIGTSGV
                     AGTVGVVGPMRIEYERMMAVVSYVSQLIERMMREESAHV"
     gene            2192..3094
                     /locus_tag="VGB76_09985"
     CDS             2192..3094
                     /locus_tag="VGB76_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY0099266.1"
                     /translation="MINENSSTPKRIPVRFVDEESNANYHVAPESSADEDDTDLTPDE
                     LGRASIYEGETEMQRRIDRGTEGDDSQGKADADERDTAGGPPRSDMPEERDEQDTHRH
                     PGANRTDGKVSADPRRAGERGGANSSLAMQAELLAAQAELEMVEAELQKVRAEQQEFQ
                     DVAARRQADFDNFRKRIERERAETYNRVVADVVGQLLPVMDNLRRAHEAESSMGATES
                     QEFRNFLHGVELISKQLNGVLENLGVEQVPTVGHPFDPHVHEAIATEQTDQFEPDTVM
                     QEIVRGYRLGEKLLRPAMVKVATR"
BASE COUNT          703 a          830 c         1005 g          567 t
ORIGIN      
        1 gttcgtcgtc gaagtagcgt cggttgagac gcgcgaagag gcggttgaga ttgtgatagc
       61 tgccgcgcga cgtggagatc atcttgcgcc cgcgacggcg acgcgcgatg tcggaggcgc
      121 gttgcacttc cggcgaaaaa gcgtaatcgc gatagacgtg ttcgtgcact tgcggcgttt
      181 tgcgtcggag cagcgaggcg acaagcataa aagcgagcgc acgctggacg cttagcggcg
      241 cgtcccgaaa gatgtctgag aggcggacat agatgcgtcc cgaacgccgt cgaatcgtgt
      301 ggttcagacc ggcataagga tagaagcgca cttcaatttc cggcggcgtc tgattgcgct
      361 caaactgccg gaaggcctcc gtgaaaagag ggctgagatg tggctgcgcg gctgacatag
      421 gtggattaat tcaggctgac ggcaaacgaa tttacgacga gttgaaacgc ctgcgaggct
      481 gaccgcacaa caagagaaaa aaacttctga caaaagcctc cgccgggaat acaattgacg
      541 ccaaacgcaa ccctatatta cgggcggctt tggcagcgcg tcaacgagcc tgtccgtcgc
      601 gcccgctaag ccttttcaga gcatcaattg tgagcgacga gtcgcgggag ggcaactggc
      661 ggccttctga gggtagtgct taagtctaat ttttagaacg atttaactaa agacgcgcga
      721 gcgttcgccg ttttgcgacg ctaactcttg tgaaatctat aaattgactt gggagagggg
      781 gagggcgcgg cgcagacggg cgtgcgtcgg caagtcgttg cgccccgttt cgtccatccg
      841 ttatactcca agcgcaaagc gtgtctgcgg cgcgtaaaat tagaacttga caggctttag
      901 acggggcggt attgattgag atgacacttg gcgaggcacg ccgcaaaagc ctctcagtgg
      961 ccttcgacgt tccccgctta ccgcttcaaa gcacaagatg gcaaatcgta gttccacatc
     1021 cacgacgact tccgagcgat tgcccgacgc gcgctcgcaa tgcgtactgt ctgccattat
     1081 caaagagcat ctgggaacgg gcgagccggt cgggtcgaga accgtctcgg aacgcctcgc
     1141 gggtgccgcc gggtggagcg cggccacgat ccgcaacgtg atggccgaac tggaagaggc
     1201 cggattggtc gagcagccgc acacttccgc cggacgcctg ccgaccgaca agggctatcg
     1261 ttattacgtg gatcacatgc tcggcgacgc gcgcctgtcg agagccgaca cggcggcgat
     1321 tgatagcctg ctgggactca ggcagggcga aggcagcatg acggctccga atcgtctgat
     1381 ggagaaagtc tcgcacctgc tgtcggaact ctcggaaaac gtcggcatcg tgatctcccc
     1441 gccgatggcc gacaatcgtt tgcagcacat cgagtttgtc caactgacgg acgggcgcat
     1501 tctcgtcgta cttgtctttg cgccgaacat catccagaac aagatcatac gcattgatga
     1561 gccgttcacg caggaagaac tggagcagac ggcgcgttat ctgaacgtgg agttcagcgg
     1621 caagaatttg cagacgatcc gtgcggagat tctcggactg atacgtgccg agaaggctct
     1681 ctatgatcgc ctgttgcaaa acgcgatgct cctgtgtgaa ctgagtctgg aaggcgaaga
     1741 ggccgcgacg ggcgacgtgt atgttgacgg tgcctcgaac attctggtca agcctgattt
     1801 cgcggacatc gagcgtctgc gtgagttgtt tcgcacgctg gaagagaaga cgcggctggt
     1861 gaaaatcttg aacgagtgtc tgacgcgcga acgcgtcttc ggcgacgtgc gcgtccagat
     1921 cgggcgcgaa ctaaagacgc cttcgatgca gaactgcgcg ctcatcaccg ccccttaccg
     1981 catcggcacg agcggcgtcg cgggcacggt cggcgtcgtc ggaccaatgc ggatcgagta
     2041 tgaacggatg atggcggtcg tcagttacgt ttcgcaattg atcgaacgca tgatgcgcga
     2101 agagagcgcg cacgtctaaa caggaaggga caggcggcgc gcaagcgagc cgctttcgtt
     2161 tacaagctga gggtttcaag gtcgaatgga catgattaac gaaaacagct ccactcccaa
     2221 gcgcatcccc gtccgttttg tggacgaaga gagcaacgcg aattatcacg tcgcgcctga
     2281 atcttctgcg gacgaagacg acactgacct tacgcccgac gaactcgggc gagcttcgat
     2341 ctacgaagga gaaaccgaga tgcagcggcg cattgatcgc ggcacagagg gagacgactc
     2401 gcaaggtaag gcggacgccg acgagcgcga cacggcgggc ggcccgccgc gctccgacat
     2461 gcccgaagag cgcgacgagc aggacacgca ccggcatccg ggcgcgaacc gtacagacgg
     2521 caaagtgagt gccgacccgc gacgtgcggg tgagcgcggc ggcgcgaatt cgtcgctggc
     2581 gatgcaggcg gagttgctcg ccgcgcaggc cgagttggag atggtggaag cggagttgca
     2641 aaaggtgcgc gccgagcagc aggagtttca ggatgtggcg gcgcggcgac aggccgattt
     2701 cgacaatttc cgtaaacgca tcgagcgcga acgcgcggag acttataacc gcgtggtggc
     2761 cgacgtggtg gggcagttgt tgcccgtcat ggacaatttg cggcgcgcac atgaagcaga
     2821 gtcttcaatg ggagcgacgg aatcgcagga gttcagaaat ttcctgcacg gcgtcgagtt
     2881 gatcagtaaa cagttgaacg gcgtgctgga aaatctggga gtcgagcaag tgccgacggt
     2941 cgggcatcct ttcgacccgc acgtacatga agccatcgct accgaacaga cggatcagtt
     3001 tgagcctgac acagtgatgc aagagatcgt gcgcggctat cgcctcggcg aaaagctcct
     3061 gcgcccggcg atggtcaagg tggcaacgag ataaagtgat gagtg
//
LOCUS       DASWNS010000282         4876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_504378, whole genome shotgun sequence.
ACCESSION   DASWNS010000282 DASWNS010000000
VERSION     DASWNS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504378"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1080
                     /locus_tag="VGB76_09990"
     CDS             241..1080
                     /locus_tag="VGB76_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099267.1"
                     /translation="MKLKIFIVLVAVVLAWVAGRSIKSGGENIVASVQQQMQHEGDDA
                     AAAHGSNERRINDSYELADGARVEVSGINGPVSIEAVEGNTAEISVTSTAGNMSDLNA
                     SQITVEHTETSLVVRGKGSNGTGFWKWLRGGSDARHNVTLKLPRRVELTTRGTNGNVT
                     IGEMEGPVQVSGVNGRVEVGGARGFAEVNGVNGGVALTITELSKDGAKVNGINGVVEL
                     RLGSDVNADLNLNGVNGQITVEAPNVEVQEQKRSKLRARVGTGGANIKANGINGGVRI
                     VGV"
     gene            complement(1209..2351)
                     /locus_tag="VGB76_09995"
     CDS             complement(1209..2351)
                     /locus_tag="VGB76_09995"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY0099268.1"
                     /translation="MRTLLPGATIGVLGSGQLGRMFAMAARRMGYLVHTYSPETDTPT
                     GQVADVEVTAPYEDLAAVRDFASRVEVVTFEFENVPAATVEAIAGIVPVRPDGATLYT
                     TQHRLREKNFLSRAGIPITPFRHVRTLAELDAALDALGCPAVFKTAGFGYDGKGQRLI
                     KSPTESADAFNSLGGQEAVLEAFVDFESEVSVVCARGADGEVAHYGVVENRHERHILD
                     CTIAPARVSVEVVREATQIAASLLEQLSLVGVLCVEFFLTRDGGKLFVNELAPRPHNS
                     GHFTIDACLTSQFEQQVRAVCGLPLGTTEQLRPAAMANLLGDLWQHGEPDWRAACALP
                     NVKLHLYGKHEPRAGRKMGHLTALAPTTDEALRLVRVARDSLTRRT"
     gene            complement(2452..3042)
                     /gene="purE"
                     /locus_tag="VGB76_10000"
     CDS             complement(2452..3042)
                     /gene="purE"
                     /locus_tag="VGB76_10000"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY0099269.1"
                     /translation="MTTKKAQRRRAGAETKKRDKKARISTPALNADASVSSAPLVSVV
                     MGSKSDWETMRGADEVLTGFDVAHECRVVSAHRTPQLMSEFASAAEARGVRVIIAGAG
                     GAAHLPGMVAAQTLLPVLGVPVESRALKGLDSLLSIVQMPAGIPVGTLAIGQAGARNA
                     ALLAVAILANEDAALRERLRAFRLEQERVVRESDLT"
     gene            complement(3039..4481)
                     /locus_tag="VGB76_10005"
     CDS             complement(3039..4481)
                     /locus_tag="VGB76_10005"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006673.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY0099270.1"
                     /translation="MAQNVPITVAHGDGIGPEIMEATLHILKEAGARLEIETIEIGEQ
                     VYLRGDTAGIPASAWESLRRTRVFLKAPITTPQGGGYKSLNVTVRKTLGLYANVRPCV
                     SYHPFVDTKHPKMDVVIVRENEEDLYAGIEHRQTAQVYQCLKLISRPGCEKIVRYAFE
                     YARRNNRQKVTCFTKDNIMKMTDGLFHKVFDEIAAEYSDIVNEHWIVDIGAAKLADTP
                     EAFDVVVMPNLYGDILSDVAAQIAGSVGLAGSANIGTSGAMFEAIHGSAPRRAGQNLA
                     NPSGLLLGAVMMLVHIDQPDVAERVHNAWLRAIEDGVHTYDIYAEGVSKQKVGTREFA
                     QAVVERLGQTPQTLKSVKYNAGGGSQTSTAGAGSGVAERKELVGVDVFLHWTEGSAEQ
                     LGETCRTLGGDDFKLSLIGNRGITVWPEGLPETFLSDHWRCRYLGAKDGATITHTQIA
                     ALLQRIADAKLDFIKTENLCNFDGKPGYSQ"
BASE COUNT          907 a         1511 c         1553 g          905 t
ORIGIN      
        1 aggatgaagg atgaacgcgg aaggatgaat ggaagcgctc aggcggtcga aaggttctct
       61 taaattcatc attcatcctt ccgcgttcat cctttcttta aaatagactg aacccgcgcc
      121 ctctttctga cgtttttctt cgtggacttg gacgagaagg aacattccaa acgcaataaa
      181 tagcttaaac tacaggcgtc cgtttcggag cgcgcgcggc gggcgggaga agagcaggcg
      241 atgaaactca aaatctttat tgtgctggtg gcggtggtgt tggcgtgggt ggccggacgc
      301 agcatcaaga gcggcgggga aaatatcgtc gcctccgtgc aacaacaaat gcaacacgag
      361 ggcgacgacg ctgcggctgc gcacggttcg aacgagcggc gcatcaacga ttcctacgaa
      421 ctcgcagacg gcgcgcgcgt cgaagtgtcc ggcatcaacg gccccgtctc aatcgaagcg
      481 gtcgagggca acaccgccga aatcagcgtg acgagcacgg cgggcaacat gagcgacctg
      541 aacgcaagtc agattacggt cgagcacacg gagacgagtc tcgtcgtgcg cggcaaggga
      601 agcaacggca cgggcttctg gaagtggctg cgcggcggca gcgatgcgcg ccataacgtg
      661 acgctcaaac tcccgcgccg cgtcgaactg acgacgcgcg gcacgaacgg caacgtcacc
      721 atcggcgaga tggaaggccc ggtgcaagtc tcaggcgtca acgggcgcgt ggaggtcggc
      781 ggagcgcgcg gcttcgcgga agtgaacggc gtcaacggcg gcgtcgcgct caccatcacg
      841 gaactatcta aggacggcgc gaaagtgaac ggcatcaacg gcgtcgtcga actgcggctc
      901 ggaagcgacg tgaacgcaga cctcaatctc aacggggtga acggccagat cacggtcgag
      961 gctccgaacg tcgaagtgca ggagcagaag cgttcgaagc tgcgcgcacg cgtcggcacg
     1021 ggcggcgcaa acatcaaggc caacggcatc aacggcggcg tgcggatcgt cggcgtctaa
     1081 gcaggcacgg gtaaatttta gcgttccagc gtgaaggccg gagagtcgaa aaactctccg
     1141 gccttcgtgc gtccggttgc cattgtcgaa agttcgcttc gtccacttcg ttcgcttcgt
     1201 tcgcttcgtc aggtgcgtcg tgtgagagag tcgcgcgcga cgcggacgag gcgcagggct
     1261 tcgtccgtgg tcggggcgag cgcggtgagg tgtcccatct ttctgcccgc gcgcggctcg
     1321 tgcttgccgt agagatgcag cttgacgttg gggagggcgc aggccgcgcg ccagtcgggt
     1381 tcgccgtgct gccagaggtc gccgagcagg ttcgccatcg cggcggggcg cagttgttcg
     1441 gtcgtgccga gcgggagtcc gcagacggcg cgcacctgtt gttcaaactg gctcgtgagg
     1501 caagcgtcaa tcgtgaagtg gccggagttg tgcgggcgcg gcgcgagttc gttgacgaac
     1561 agtttgccgc cgtctcgcgt caggaaaaat tccacgcaga gaacgccgac cagcgagagt
     1621 tgttcgagca ggctggcggc gatctgcgtc gcttcgcgca cgacctctac agagacgcgc
     1681 gccggggcga tagtgcaatc gaggatgtgg cgttcgtgcc ggttttcgac cacgccgtaa
     1741 tgcgccacct cgccatccgc gccccgcgcg cacaccaccg acacctcact ctcgaaatcg
     1801 acgaacgctt cgagcactgc ctcctgaccg ccgagcgagt tgaacgcgtc cgcgctctcc
     1861 gtgggcgact tgatcaggcg ttggcctttg ccgtcgtagc cgaagcccgc agtcttaaag
     1921 actgcggggc agccaagcgc gtcgagcgcg gcgtcgagtt cggcgagcgt gcgcacgtgt
     1981 cgaaagggcg tgatggggat tccggcgcgg gagaggaaat tcttttcgcg caggcgatgc
     2041 tgcgtggtgt agagcgtcgc gccgtccggc ctgacgggga cgatcccggc gatggcctcc
     2101 acggtcgccg ccggaacgtt ttcaaactcg aaagtcacta cttcgacgcg gctggcgaag
     2161 tcgcgcaccg ccgcgaggtc ttcgtaaggc gcggtcactt ccacgtctgc gacctgcccg
     2221 gtcggcgtgt cggtttcggg cgagtaagtg tgtacgagat agcccatgcg gcgcgcggcc
     2281 atggcaaaca tgcgaccgag ttggccgctg ccgagcacgc cgatggttgc gccgggaagc
     2341 agcgtgcgca tacttgtgcg ggaagagggc gcgtgattca cggtttacgc caaagcgata
     2401 aagttggttt tcgctatctg agacaacctg ttttcgctat ctgaaacaac ttcaagttaa
     2461 atctgactcg cgcaccacac gctcctgctc aagccggaac gcgcgcaggc gttcgcgcaa
     2521 agcggcgtct tcgttggcga ggatggcgac ggcgagcaac gcggcattgc gcgccccggc
     2581 ctgcccgatg gcgagcgtgc cgacgggaat cccggcgggc atctgcacga tggagagcag
     2641 cgaatccaga cccttgagcg cgcggctctc cacgggcacg ccaagcaccg gcaacaaagt
     2701 ctgcgccgcg accatccccg gcagatgcgc cgccccgccc gcgcccgcga tgatcacacg
     2761 cacgccccgc gcctccgccg cagacgcaaa ctccgacatc aactgcggcg tgcgatgcgc
     2821 cgagacgacc cggcactcgt gcgcgacatc gaaccccgtc aacacctcgt ccgccccgcg
     2881 catcgtctcc caatcggact tgctgcccat gacgaccgag acgagcggcg cggaagaaac
     2941 ggacgcgtcc gcgttgagcg cgggtgtgga gatgcgcgct tttttgtcac gctttttcgt
     3001 ctccgcgccc gcgcgccgcc gctgagcctt cttggtcgtc attgcgagta gccgggcttg
     3061 ccgtcgaagt tgcagaggtt ctcggtcttg atgaagtcga gtttggcgtc ggcgatgcgc
     3121 tggagcagcg cggcgatctg cgtgtgcgtg atggtcgcgc cgtccttcgc gccgaggtag
     3181 cggcaacgcc agtggtcgga gagaaaagtc tccggcagcc cttccggcca gacggtgatg
     3241 ccgcgattgc cgatcaggga taacttgaaa tcgtcgccgc cgagcgtcct gcacgtctcg
     3301 cccaactgtt cggcggagcc ttccgtccaa tgcaaaaaca cgtccacgcc gaccagttct
     3361 ttgcgctctg cgactccgct gcccgcgccc gccgtgctcg tttggctgcc gccgcccgcg
     3421 ttgtacttga cggacttcaa agtctgcggc gtctgtccca acctctcgac gaccgcctgc
     3481 gcaaactcgc gcgtccccac cttctgcttg ctcactcctt cggcgtagat gtcgtaagtg
     3541 tgtacgccgt cttcgatggc gcgcagccat gcgttgtgga cgcgctcggc cacgtccggt
     3601 tgatcgatgt ggacgagcat catcaccgcg cccagaagta agcccgaagg attggctaga
     3661 ttctgcccgg cgcggcgcgg cgcggagccg tgaatcgctt cgaacatcgc gccggaggtt
     3721 ccgatgttgg ccgaacctgc gaggccaacc gagcctgcga tctgtgcggc cacgtcggag
     3781 agaatgtcgc cgtagaggtt cggcatcacc accacgtcga aggcttcggg cgtgtcggcg
     3841 agtttggccg cgccgatgtc cacgatccag tgctcgttca cgatgtcgga atactcagcc
     3901 gcgatctcgt cgaagacttt atggaagagg ccgtcggtca tcttcatgat gttgtctttc
     3961 gtgaagcagg tgaccttctg gcggttgttg cgccgcgcgt attcgaaggc gtagcgcacg
     4021 atcttttcgc agccggggcg cgagatgagt ttcaggcatt gatagacctg agcggtctgc
     4081 cggtgctcga tccccgcgta caggtcttcc tcgttttcgc ggacgataac cacgtccatc
     4141 ttcgggtgct tcgtgtccac gaacggatga taagagacgc acgggcgcac gttcgcgtag
     4201 aggccgagcg tcttcctgac ggtgacgttg aggctcttgt agccgccgcc ctgcggcgtc
     4261 gtgatcgggg ctttcaaaaa gactctggtg cggcgcagag actcccacgc gctcgcgggt
     4321 atccccgccg tgtcgccgcg cagatagacc tgctcgccga tctcaatcgt ctcgatctca
     4381 agcctcgcgc cggcttcttt caggatgtgg agcgtcgcct ccatgatttc aggaccaatg
     4441 ccgtcgccgt gcgccaccgt gataggaaca ttctgtgcca tgatttaact gaagcacctt
     4501 tcgctacatt tgaattttaa gaagacaact caggtggccg ctagcttact acttttcacc
     4561 cgtcgcccga aagttgcgcg tcgcatacgc ccgccgcaag atgcgccaac tctcgccgcg
     4621 cgccgcccgc agcccataaa gatcgtgtaa cttctccgac acgaaacacg cacgccgccg
     4681 ttattcctct ccgcgcgcaa atttagtatg atgccgcttg tgtcagccct ccgcgcgaaa
     4741 gcagatgacg agaagttttt gcgccgcgaa gtggcggacg cggagcctcc ccacaaaacc
     4801 ccctttgcaa cttacgcccg cgaactatgc tcctcgcgcg ccgagagaac atgccagcac
     4861 tgaaatctga cggtta
//
LOCUS       DASWNS010000283         6967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_400103, whole genome shotgun sequence.
ACCESSION   DASWNS010000283 DASWNS010000000
VERSION     DASWNS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400103"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /gene="cutA"
                     /locus_tag="VGB76_10010"
     CDS             <1..329
                     /gene="cutA"
                     /locus_tag="VGB76_10010"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEY0099271.1"
                     /translation="DAAITVMLTAGSREEAARLAEMLVGARLAACVQILPEMESVYHW
                     KGEVRREPEFLLLAKTTEAHFALLEREVRALHSYETPEIIALPITAASAPYLEWLTGT
                     LKPGDE"
     gene            complement(439..1185)
                     /locus_tag="VGB76_10015"
     CDS             complement(439..1185)
                     /locus_tag="VGB76_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY0099272.1"
                     /translation="MAKTKNGNRKGAHVTQDEQLLETPKLDEFKHTDTWRVFRIMGEF
                     VEGFDELATVTRGVSIFGSARTPPDSPHYAAAQETAALLVRAGFAVITGGGPGIMEAA
                     NRGAFEAKGVSIGCNIELPFEQRPNVYQTRSLTFNYFFVRKTMFVKYSTAFVIFPGGF
                     GTLDELFEALTLIQTRKVRNFPVVLVGRQFWSGMMDWLRGTLLAEQTISQEDLALIHL
                     TDSPAEAVEIVVRSQDALRKLDKTVSNDIA"
     gene            complement(1281..2042)
                     /locus_tag="VGB76_10020"
     CDS             complement(1281..2042)
                     /locus_tag="VGB76_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEY0099273.1"
                     /translation="MLENKFGVVFGVANKRSIAWASALALSGAGARLAFTYQNERLRE
                     NVESLTKAMPGAVLSQCDVVKPEEVEQTFALVKENFGRLDFLVHSLAFAPREALEGEY
                     VNTSLEAFQTALEISAYSLATLARASAPLMNEGGSIVSMSYYGAEKVVPNYNVMGVAK
                     AALEASTRYLASDLGKHNIRVNAISAGPLNTLAARGVGNMGAMLKHHAERAPLRRNVE
                     ASEVGNTALFLCSPLSSGITGEVIHVDCGYNIMAF"
     gene            complement(2138..3127)
                     /locus_tag="VGB76_10025"
     CDS             complement(2138..3127)
                     /locus_tag="VGB76_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY0099274.1"
                     /translation="MADFESISGGENNGTPVRCRFCGQLNQTRADVTGSAKCGRCRLP
                     LSGAPHKKFAELDKDAYIHPADSRALATLRAIPGIDTAVKKLLSVTGESAIRVIFMAS
                     AVRVTPHQCPDLYAKLQIACTTLGVDMPELFVQQSPMVNAFTGGVTRPVIVLYSGLLE
                     RLTDEEVLAVVAHEVGHIHAEHVLYLTVAHLLEALANVAIAAAPVASLVAQILSTTMR
                     SALLAWSRRAELSCDRAALLVTQDADVIGRTMMKLCGGTFASKVDYEQFLVQARDFQK
                     NYDDKALDRFWADIITSGLSHPFPVWRTSEILKWVESDQYERLMNGAPEIAAA"
     gene            complement(3243..3641)
                     /locus_tag="VGB76_10030"
     CDS             complement(3243..3641)
                     /locus_tag="VGB76_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099275.1"
                     /translation="MRKQSIKVKLAATFFGLLLTTCAFAAQEGLTKRVRFARGRTTAT
                     LKNSVVRGTVDRYTLGARAGQKMSVSITSVERNAVFTIYTPGGGTLEGAGEGQDASNW
                     SGELPASGDYVIEVGGTRGNATYTLKVTVR"
     gene            complement(3664..5325)
                     /gene="purH"
                     /locus_tag="VGB76_10035"
     CDS             complement(3664..5325)
                     /gene="purH"
                     /locus_tag="VGB76_10035"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY0099276.1"
                     /translation="MPEQNPDGLRNIRRALLSVSDKTNLVEFARALSRFGVELLSTGG
                     TAKALREAGVEVRDVSEVTGFPEMLDGRVKTLHPRIHGGLLAVRDNHEHMSALGEHGI
                     EPIDMVVINLYPFRQTIAREGVTLAEAIEQIDIGGPAMVRSAAKNYTDVAVVTSPEMY
                     ERVLSELEEHKGALTLNTRAHFAAQAFGSTAHYDAAIWAYLQRTGFGDDPASTSHAQP
                     GGGIGAGAAGYPDAPSRSLEDLRDAPDLAQWSLRKTADLRYGENPHQSAALYATGASG
                     GIGAAEILSGKEMSYNNYVDADAAWQLVCDYDETACAIIKHTNPAGVGAGATAEEAYR
                     LALATDPVSAFGGVVAFNRPVDAATARALTEIFTEVVIAPNFDEAALEVLRAKKNLRV
                     LRAGVPRRAGGLECRQITGAMLLQTRDTHRLTRDELKVVTRRAPTVEEIDALLFAWTV
                     CKHTKSNAIVYARAGQTVGVGAGQMSRVDSVRIGAARAVLPVAGSVLASDAFFPFRDG
                     IDEAARHAIKAIIQPGGSVRDEEIIAAADEHDLAMIFTGIRHFKH"
     gene            complement(5481..6491)
                     /locus_tag="VGB76_10040"
     CDS             complement(5481..6491)
                     /locus_tag="VGB76_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0099277.1"
                     /translation="MTLPRLLYTRRHVFTRAAFHLFLFSLFVLPPGTGACAQTIDASD
                     DEVLRVSTDLVTVPVVVTDRRGRRFANLTRQDFEVRDGGRPVKLEYFSSGTERVAIVF
                     ALDASGSAREHIARQTEAALAMFAHFGSNSRIAVLPFAAQADFALPFTTDIRRAREAF
                     QLSARRNQQTAIFDAASVAVRAFNVAGADARERRIVILSSDGLDTASRTPPASVINEA
                     RLRGVSIYVIHFPLFIPRDGKLAVRRPAKGFRELAEQTGGHYFLLGDARSALDPRAAP
                     DLAPIFRAITEDLQSQYLLGYYLDAAARNQSNNHQIQVSLTPAHKRLRVQLLREGYNL
                     KQ"
BASE COUNT         1336 a         2017 c         2229 g         1385 t
ORIGIN      
        1 gtgatgcagc gatcacggtg atgttgacgg caggcagccg ggaagaagcg gcgcggctgg
       61 cggagatgct cgtcggcgca cgcctcgcgg cctgcgtgca gatacttcct gagatggagt
      121 cggtctatca ctggaagggc gaggtgcggc gcgagccgga atttctgttg ctggcgaaga
      181 ctaccgaggc gcacttcgct ttgcttgaac gcgaagtgcg cgcgctgcac agctacgaga
      241 cgcccgagat catcgctctc cccatcaccg ctgcttccgc gccctacctc gaatggctga
      301 ccggcacgct caagcccggc gacgaataaa aacgctcgcg ggctggcaac gttcagttgt
      361 cagtcgcctg tcgcaatcac gcgactggag attgggctga acaggggtca aagacaactg
      421 ccaactgaca ctgcttcctc aggcgatgtc gttcgagacg gtcttgtcca acttgcgtag
      481 cgcgtcctga ctacggacga caatctcgac ggcttcggcg ggcgagtctg tgagatggat
      541 gagggcaagg tcttcctgcg aaattgtctg ttctgccagc agcgtgcctc tcagccagtc
      601 catcatcccg ctccaaaatt gtctgccgac gagcacgacg gggaagtttc tcaccttgcg
      661 cgtctggatg agcgtgagag cttcgaagag ttcgtcgagc gtgccgaagc cgccggggaa
      721 gatgacgaag gcggtgctgt atttgacgaa catcgtcttg cgcacgaaga agtagttgaa
      781 ggtgagcgag cgcgtctggt aaacgtttgg ccgctgctca aaagggagtt cgatgttgca
      841 accgatggag acgcctttgg cctcgaacgc gccacggttg gcggcctcca tgatgccggg
      901 accgccgccc gtaatcaccg cgaagcccgc gcgcacgagc aatgcggctg tctcctgtgc
      961 tgctgcgtag tgcgggctgt cgggcggcgt gcgagccgag ccgaagatgg agacgccgcg
     1021 cgtcaccgtc gcgagttcgt cgaagccctc cacgaattcg cccatgatgc gaaaaacgcg
     1081 ccacgtgtca gtatgcttga actcgtcgag cttcggagtc tcaagtagtt gctcgtcctg
     1141 cgtgacgtgc gcgcctttgc ggttcccgtt ttttgtcttt gccatttagg tgcgagggct
     1201 gaggtcagaa gtacggaggt tggaagaagc acgcgttttg tctctactga cctctaaact
     1261 ccggcctccg acttctgctt ttaaaacgcc atgatgttgt agccgcaatc gacgtggatg
     1321 acctctccgg tgatgcctga cgagagcggg ctgcacagga agagcgcggt gttgccgacc
     1381 tcgctcgctt cgacgttgcg acgcaacggg gcgcgctcgg cgtggtgctt gagcatcgcg
     1441 cccatgttgc cgacgccgcg tgcggcgagg gtgttgagag gtcccgcgct gatggcattg
     1501 acgcgtatat tgtgcttgcc gaggtcgctc gccagatagc gcgtgctggc ttcgagcgcg
     1561 gctttcgcca cgcccatcac gttatagttc ggcaccactt tctccgcgcc gtaataactc
     1621 atggagacga tgctcccgcc ttcgttcatc aggggcgcgg aggcgcgggc gagagttgcg
     1681 agcgagtagg cgctgatctc aagcgcggtc tgaaacgctt cgagagaggt gttgacgtat
     1741 tcgccttcca acgcctcgcg cggcgcaaag gcgagactgt ggacgaggaa atcgagccgc
     1801 ccgaagtttt ctttaaccag cgcgaaagtc tgttcaacct cttccggctt caccacatcg
     1861 cactgcgaaa gcaccgcgcc cggcatcgcc ttcgttaaac tttccacgtt ctcccgcaga
     1921 cgctcgttct ggtaagtgaa cgcgagccgc gcccccgcgc cggagagcgc aagagccgaa
     1981 gcccatgcaa tcgaacgctt attagccacg ccgaacacga cgccgaactt attctctaac
     2041 attcagggaa gcctccgcat tcagcgagca gtaaacagtg agagagccat aaaacaggaa
     2101 tagctttaga ctgctcactg ctcgctgcac acttacttta cgctgccgcg atctccggcg
     2161 cgccgttcat caagcgctcg tattgatcgc tctccaccca cttcaaaatt tccgacgtgc
     2221 gccagacagg gaacgggtgc gagaggccgc tcgtgatgat gtcagcccag aagcggtcga
     2281 gggctttgtc gtcgtagttc ttctggaagt cgcgcgcctg gacgaggaat tgttcgtagt
     2341 caactttgga ggcaaaggtg ccgccgcaga gtttcatcat cgtgcgcccg atcacgtctg
     2401 cgtcctgcgt gacgagcagc gcggcgcggt cgcacgacaa ctcggcgcgg cgcgaccatg
     2461 cgagcagagc cgaacgcatg gtcgtagata aaatctgcgc gacgagactt gccaccggag
     2521 ccgccgcaat cgccacgttg gctaacgctt cgagcagatg cgcgaccgtc agatagagca
     2581 cgtgctcggc gtgaatgtgg ccgacttcat gcgcgacgac ggcgagcacc tcttcgtccg
     2641 tgagacgttc gaggaggccg gaatagagca cgatgacggg gcgcgtgacg ccgcccgtaa
     2701 acgcgttgac catcggcgat tgctgcacga aaagttccgg catgtccacg ccgagcgtcg
     2761 tgcaggcgat ctgtaacttg gcgtagaggt ctggacattg atgtggcgtg acgcgcaccg
     2821 cgctcgccat gaagatgacg cggatggccg attcgcccgt cacggacagc aactttttca
     2881 ccgccgtatc gatgcccgga atcgcgcgca gcgtagcgag cgcgcggctg tcagcgggat
     2941 ggatgtaagc atctttgtcg agttcggcga attttttgtg gggcgcgccg gagagcggca
     3001 ggcggcagcg tccgcacttc gcgctacctg ttacgtcggc gcgggtctgg tttaactgcc
     3061 cgcagaagcg gcaacgcacg ggcgtcccgt tgttttcgcc gccggagata gattcaaaat
     3121 cagccatgag ttcaaccctt cgtcaaaaca ggtggaataa attttctggg agacaggaac
     3181 gcccaagtat caacgattgc gccgcgccac tgcaagcgtg cacaaacgtc cggggcgacg
     3241 gctcaacgaa ccgtgacctt gagcgtgtag gtcgcattgc cgcgcgtgcc gccgacctcg
     3301 atcacgtagt cgccgctggc aggtagttcg ccgctccagt tcgaggcgtc ctgcccttcg
     3361 cccgcgcctt cgagcgtgcc gccgccgggc gtgtagatgg tgaagaccgc attgcgctcg
     3421 accgaagtga tgctgacgga catcttctgc ccggcacgcg cgccgagcgt gtaacgatct
     3481 accgtgccgc gcacgacaga gttcttcaaa gtcgccgttg tgcgcccgcg cgcgaaacgc
     3541 acacgcttcg tcaaaccttc ctgcgcggcg aacgcacagg tcgtgagcag cagcccgaaa
     3601 aaagtcgccg ccagttttac tttgattgat tgcttcctca tcgtcttctc agtctccctg
     3661 tgttcagtgt ttgaagtggc ggatgccagt gaagatcatc gccagatcgt gttcgtctgc
     3721 ggcagcgatg atctcttcgt cgcgcacaga cccgccgggc tggatgatgg ccttgatggc
     3781 gtggcgtgcg gcttcgtcta tgccgtcgcg gaaagggaag aaggcatcgg aggcgagcac
     3841 ggagcctgcg acgggaagca ccgcgcgcgc cgcgccgata cgcacggaat caactctcga
     3901 catctgcccc gcgccaaccc cgaccgtctg ccccgcacgc gcatagacga tagcgtttga
     3961 cttcgtatgc ttgcagaccg tccaggcgaa gagcagcgcg tcaatctctt cgacggtcgg
     4021 cgcgcgtcga gtcacaactt taagctcgtc gcgcgtgaga cggtgcgtgt cgcgcgtctg
     4081 caaaagcatg gctccggtga tctgcctgca ctcaagcccg cccgcacggc gcggcacacc
     4141 cgcgcgcaac acgcgcaaat tcttcttcgc acgcaagact tcaagcgcgg cttcgtcgaa
     4201 attcggggcg ataacgacct cggtgaaaat ctccgtcagg gcgcgagccg tcgccgcgtc
     4261 aaccgggcga ttgaaggcca cgacgccgcc gaaagccgaa acggggtcgg tggcgagcgc
     4321 gagccggtaa gcctcttccg ccgtcgcgcc cgcgccgacg cccgccgggt tcgtgtgctt
     4381 gatgatggcg caagccgtct cgtcgtagtc gcagacgagt tgccacgccg cgtccgcatc
     4441 gacgtagttg ttgtatgaca tctccttgcc cgacagtatc tcggctgcgc cgattcctcc
     4501 gcttgctccc gtcgcgtaga gcgcggcgga ctggtgcggg ttctcgccgt agcgcaggtc
     4561 ggctgtcttg cgcagcgacc attgcgcgag atcgggcgcg tcccgcaaat cttcgagcga
     4621 acgggacggg gcgtcgggat aaccggctgc gcctgcgccg atgccgcctc cgggctgcgc
     4681 gtgcgaagtc gatgccgggt cgtctccaaa tcccgtgcgt tgcagataag cccagatggc
     4741 ggcgtcgtaa tgcgccgtgc tcccgaaggc ttgagcggcg aagtgggcgc gcgtattgag
     4801 cgtgagcgcg cccttgtgtt cctccaactc ggagaggact cgttcataca tttcgggcga
     4861 ggtgacgacg gccacgtccg tgtagttctt cgccgccgag cgcaccatcg cggggccgcc
     4921 gatgtctatc tgctcaatcg cctccgcgag cgtgacgcct tcgcgggcga tggtctgccg
     4981 gaagggatag agattgatga cgaccatatc tatcggctcg atgccgtgct cgccgagcgc
     5041 gctcatgtgt tcgtgattat cgcgcacggc gagcagcccg ccgtgaatgc gtgggtggag
     5101 ggtcttgaca cgcccgtcga gcatttcggg aaagcccgtg acttcggata cgtcgcgcac
     5161 ttcgacgccc gcctcgcgca aggctttcgc cgtgcctccg gtgctcaaaa gttcgacacc
     5221 gaagcgactg agcgcacgcg caaactcgac gaggttcgtt ttgtccgaga cgctcaacag
     5281 cgcgcggcgt atgtttcgca atccatctgg gttctgttca ggcatatttt taagtgacga
     5341 gtgacgagtg acaagataaa acagtgaatc gtgaatcgta aatcgtgaag aggcgaaatc
     5401 ggattgagtc aatttgcgtt ttacagttca cgcttcaaac ttcgcgtttt gaacttcacg
     5461 ccttattaac ttgtcgctcg tcactgtttc aaattgtagc cttcgcggag caactgcacg
     5521 cgaaggcgtt tgtgtgccgg ggtcaggctg acttgaattt gatggttgtt gctctgattg
     5581 cgcgcggcgg cgtcgaggta atagccgagc aggtactggc tctgtaaatc ttcggtgatg
     5641 gcgcgaaaga ttggcgcgag atcgggagcg gcgcgcgggt cgagggcgga gcgcgcgtcg
     5701 ccgagcagga aatagtggcc gccggtctgt tctgcgagtt cgcgaaaacc tttagcggga
     5761 cggcgcacgg cgagtttgcc atcgcgcggg ataaagagcg gaaaatgtat gacgtagatg
     5821 gagacgccgc gcaggcgtgc ctcgttgatt acggacgcgg gcggcgtgcg actggccgtg
     5881 tcgaggccat cgctggataa gatgacgatg cggcgttcgc gtgcgtcggc tccggcgaca
     5941 ttgaaggcgc ggacggcgac ggaggcagcg tcgaagatgg ctgtctgctg gttgcggcgt
     6001 gcggagagtt ggaaggcttc gcgcgcgcgt ctgatgtctg tggtgaaggg gagcgcgaag
     6061 tcggcctgcg ccgcaaacgg gagcacggcg atgcgcgagt tgctgccgaa atgggcgaac
     6121 atggcgagcg cggcttcggt ctgtctggcg atatgctcgc gcgcgctgcc ggaagcgtcg
     6181 agcgcgaaaa caatcgcgac gcgctccgtg ccggaggaga aatattcgag tttgacaggc
     6241 cgcccgccgt cgcgcacttc gaagtcttgc cgcgttaaat ttgcgaagcg tcgcccgcgc
     6301 ctgtctgtta cgacgacggg gacggtgacg agatcggtgc tgacgcgcaa gacttcatca
     6361 tcgctcgcgt ctattgtttg cgcacatgcg cctgtgcccg gcgggaggac gaaaagagag
     6421 aacaggaaaa gatggaaagc ggcgcgtgtg aagacgtgtc gccgggtata gagtaaacga
     6481 ggaagggtca tgccgccgtc aggcctgaaa caaaaaccgc ccgaagaaca gtatcgtcag
     6541 gatgacagca atagtgatga tgatgatctt tacaaggctc acgacttgat cctcaaactg
     6601 actggatgcc agcggtaatc tccagcgagg gctgtattct atcggtgcat tgagagaagt
     6661 gtcaacgctt tgggcaaata tggcctgccg cacgcttgat tttgcctgga aagcaaaact
     6721 ttagacgtgc ggcaggccgg taaagttcca agtttttcgg tgccgcaggt gtagcccgat
     6781 tcgcttaact ctgcgacttc ggccttgagc ggcttgccgc tatttgaaac gcagggacag
     6841 ccgcccgctg aagagtcgcg gatagccgat gtgcgtgccg ctgaagaccg actcgaagtt
     6901 gtagagaaag agcgtgttgg tcaaattctg gacatcgaac tgcacgttga gcgacgtgcg
     6961 acgttcg
//
LOCUS       DASWNS010000284        11581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_480012, whole genome shotgun sequence.
ACCESSION   DASWNS010000284 DASWNS010000000
VERSION     DASWNS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11581
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480012"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(886..3420)
                     /gene="ppsA"
                     /locus_tag="VGB76_10045"
     CDS             complement(886..3420)
                     /gene="ppsA"
                     /locus_tag="VGB76_10045"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEY0099278.1"
                     /translation="MKNLIKDLVLDLSEVGADDISLVGGKCASLGELFRALVPKGVRA
                     VDGFATTSEAYRHLLATNNLETRLRKLLDGLDCEDLAALSAAGREARALMLETPLPYD
                     VREAILDAYRRLCQRTRSACEVAVRSSATAEDLPDASFAGQQDTILNVRGEAELLAAC
                     QQCFASLWTDRAISYRTARSFDHFEVALSIGVQPMVRSDLATSGVMFTLDTESGFRDV
                     VTINGAWGLGEAIVQGMATPDEWVVFKPTLHEGARAVVSRRLGSKEVKMSYGPDGSGT
                     RIREVVAAQRRRFSLEDYEVLQLARWACLIETHYSERAGHDQPMDIEWAKDGITGELF
                     ILQARPETVHSTNRENFVLNYRLTGSHQEPLVTGQAVGEKIGHGRVHVLADPDHLAEF
                     SAGDVLVTSMTDPAWEPIMKRAAAIVTDRGGRTCHSAIISRELGLPCIVGTGDGTRKL
                     PNGMPVTVSCAEGAQGNIYDGLIDFVIDKHTVSDAGRPRTQVMMNVGDPDHAYALAGL
                     PNDGVGLARLEFIINNHIGIHPMALVNYPHIAREEDIREIEERIGEEDPREFFVRCLA
                     EGIGRIASAFYPKPVVVRMSDFKSNEYARLIGGQQFEPVEENPMLGFRGASRYYDERY
                     REGFHLECRALRRVREDMGLVNVKAMIPFCRTVEEGERVINLMREYGLKQGERELEIY
                     AMCELPANVVFADEFLRIFDGYSIGSNDLTQLALGLDRDSEMVAHLFDERNGAVQLMI
                     AQAIDAAHRAGKKIGICGQAPSDYPEFAHFLVERGITSISLNPDTVIQTTQKIIEAEL
                     AVKANWRVPTPFKVQAGNADGASLKAGAPAEAIELPGGFTAEAISG"
     gene            complement(3761..4192)
                     /locus_tag="VGB76_10050"
     CDS             complement(3761..4192)
                     /locus_tag="VGB76_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099279.1"
                     /translation="MFHIRQRRWADCGVATIAMAAGVAYERVLESVAPDCFVHGLWSG
                     EMLHALLKITGRDWSLAEFDGETTLKDFDFPEGPAIVCIDRPEMAHYWARHYAVVAGN
                     VIYDPLLKAPLSMEEAKGDHHGAWRVVGLMTGGLASTSSKV"
     gene            4184..4510
                     /locus_tag="VGB76_10055"
     CDS             4184..4510
                     /locus_tag="VGB76_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099280.1"
                     /translation="MKHLPHHSPFIFSCSRRGAHDSIGRRHQQLAGAERIHIFRNHLP
                     PFGRDFNETEDRARTLQRYGIATAGIGDDITDGQRQLEVVDGYGVKRCKVYADVEAPA
                     QEVMIG"
     gene            complement(4809..5045)
                     /locus_tag="VGB76_10060"
     CDS             complement(4809..5045)
                     /locus_tag="VGB76_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099281.1"
                     /translation="MNVAKNGIRVIKREEREQRAQQKMETTKQETEAETELRRTQERE
                     LASSVAEWVNDFLRRRHGGFPEEMGGQLRALFER"
     gene            complement(5092..7389)
                     /locus_tag="VGB76_10065"
     CDS             complement(5092..7389)
                     /locus_tag="VGB76_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY0099282.1"
                     /translation="MGVETTGNARIELWGGVECTVNRVGDEYFDQLERNGHAERIEDL
                     DLFAGLGIRAIRYPVLWERTAPDGLETADWSWADSRLARLRELGIRPIVGLVHHGSGP
                     RHTSLVDPAFPEKLAEYARGVAERYPWVDAYTPVNEPLTTARFSGRYGLWYPHGQDRR
                     TLCEALLIQCRAVALSMRAIREINPAAQLIQTDDLGKTYSTRKLRYQADLENERRWLT
                     FELLTGRLNRHHPMWTYFTGVGIDEAELDWFTDNPCPPDVVGINHYLTSERFLDERLE
                     RYPACSHGGNMKDRYADVEAVRVCAEGLAGHRTLLRETWERYGLPIAVTEVHLGCTRE
                     EQLRWFKEVWETCQDLREEGVDVRAVTAWSLLGAFDWNKLLTCSAGFYEPGVFDVRGT
                     HRRPTAIAHLLRELAAGREFNHPAIEGPGWWQRLERLLYTPVRREPRAAATAKQPVRG
                     ASSRKSSTRPLIIAGATGTLGSSFARLCEVRGISYRLLSRCQMDIADQESVRAALNEY
                     DPWAIVNAAGYVRVDQAEHEPEACFRENRDGAATLAVACAERGISLLTFSSDLVFDGE
                     VSEPYLESGRTVPLNVYGQSKAEAERRILEVHPAALVVRTSAFFSPWDEHNFVTAALR
                     TLASGETFKAADDAIVSPTYVPDLVNASLDLLIDGERGIWHLANSGAVTWAELARRAA
                     QLAGLDASGVQGCATRALDGTTARRPMYSVLGSERGVLLPSLDEALKRYVNECTASWK
                     TNLSSGQVEGAEAPANRWSTARQSA"
     gene            complement(7480..8892)
                     /locus_tag="VGB76_10070"
     CDS             complement(7480..8892)
                     /locus_tag="VGB76_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0099283.1"
                     /translation="MKDSKKMDGGMRYDNQDGLRRGAAATSDMNGLKVASSNGRHAGA
                     RSLPARSRSTDDGFALAAEPDARGFESAPDLVCLSHLRWDFVYQRPQHLLSRCAKARR
                     VFFIEEPVYDNGPVRMHVSERECGTVVMVPWLPERLQDDPARDAALQDLLDRFFVEQK
                     IDDYLLWYYTPMAMAFTQHLRPRAVIYDCMDELSGFKDAPPALRFCEGELFRRADLVF
                     TGGQSLYESKRKQHPKHPHIHAFPSSIDAAHFAQARNAMSEPADQAGIPHPRLGFFGV
                     IDERIDLELLDRISAARPDWQFVIVGPVVKIEQASLPRRANIHYLGGKAYSELPEYLA
                     GWDVALMPFALNDATRFISPTKTPEYLAAGLPVISTSIRDVVRPYGEKSLVRIADTAE
                     AFIGAAEAVMTEDAAARQAVVDKFLSQTSWDMTWARMSELIENAIDDGRVTAEATPSR
                     NLKQRAAGAESAAVNFSVAS"
     gene            complement(9167..10243)
                     /locus_tag="VGB76_10075"
     CDS             complement(9167..10243)
                     /locus_tag="VGB76_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099284.1"
                     /translation="MRGIERTFLLSLLVGLLPCGNFPTAAQGRNSGSFPAASARGAAR
                     RLADAARAETTAAILSEVWPEGVSRVRLSGIGRGKGIVFSPDLSVPTNRKFYERLGFT
                     YFEDADWRVVLGQIRTHNQSQPDNPVEVLILESHGTNGHGLKLQAGHAAKDARSYLSV
                     GALQERLEGTGIRLSIITACNAGRLFRPHIYKRLNTRTGDPLFLPATLGVVNASPNYD
                     PSKSALRVVYPSKSSLETTNEGDTSELSPAAQKALSISGEFERQSDADATPAPTHFRF
                     VVSDILAQMLLRDPRFRLNLGGYVTKKSNDNFSDGESEILFQQFLDYLDEIARHEYPV
                     AGDELLDKSPAAEEHREISRPFRL"
     gene            complement(10302..11429)
                     /locus_tag="VGB76_10080"
     CDS             complement(10302..11429)
                     /locus_tag="VGB76_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099285.1"
                     /translation="MKKAFVSVLPGVALLFPLLTHTPQSRPSLTLAPREVSSTISTAR
                     RPEQERLDRLHAQIRVAKKLLAVQPAPPADAVHVAFEDGATDGLNLLTLPKEFFRRRE
                     AEASVKSSRGHLVHVRIVRANGVNTAVTISDAGGRAHVPLLIRYPIKQGGTTHSAYYT
                     SVHPALESTELAREGRAYVRRMLDEAAGRLAATGVLVAPDIIDVAERLCIVEHADHDR
                     FRTEDQAALFAEIASLYALNAGDTYRYSVSSAGAGGMVQMIPSTYRMVRERHPEARLN
                     PDFASGMRDHSNALEAMLLYMHDTWNDLIAEEAVVNALNTRTATQVELLAAGYNSNPA
                     RLAGYLARGGESWRTLIPRETQAYLQIYASVVNRMPASRLD"
BASE COUNT         2228 a         3529 c         3473 g         2351 t
ORIGIN      
        1 taaatctctt ccggatgtcg aatgttgagt tgggaaataa ccgggagtag ctaacgtgag
       61 cagtataaca ttaacctgcg cgcctgccac acaagacctg actcagcggg tctgaggagc
      121 accaatcttc acggggcatt cgcccgctgc ttaaccttct gcacgcaatt gaccataaat
      181 ttttatgatt ccgcgtcatg attgtcgaaa agaaatggtt gagtccggct acgggtgcgt
      241 cagggttgtt aattgcacgg agatgaaagc gcaagcccgc gagcgttaga ccgtcggacg
      301 gagagcgatt gacatcctag cctcttcaac acacaaccga cttgaccgtt aacccgtctc
      361 gtaccaagtc gtacagacaa ccaatgccgc ccgcccctat aaatcttctt gcctaacggg
      421 caaggggcat cagtgcggag ccgccggggt ggtcgcacat agttgtagcc ctcggcggtc
      481 gtactgactc acatccgctc taacccgaca caggctgagg agacacttga gacgggcttc
      541 ggatgcccta aagatcaagc caggggagat gtcagagtta gcatgaatca caacgagcag
      601 ttacttcgga aggcagcgat ttacggagcg atacttctaa cggttgtctt caccgcttta
      661 ctctggaagg ggtaatcggc agcggttaac cgtcaccttc attccgtcac cttcattcat
      721 taaacaacgc gaagtggaaa ggggcaggat tacacgcctc acgcccgcgt ccgctgtacg
      781 tctgtgaaca gccgcccgtg accggcctct gttcagacgc tcataggcag agcgcgggcg
      841 cgcgtctctc acgattctga cgccacctca ggcgacatca caccgttagc cgctgatagc
      901 ttctgccgtg aagccgccgg gcagttcaat cgcttcggcg ggcgcgcctg ccttcaacga
      961 tgcgccgtca gcattcccgg cctgtacttt gaagggcgta ggcactctcc agttagcctt
     1021 cacggcaagc tcggcctcga tgatcttctg cgtggtctgg atgaccgtgt cggggttgag
     1081 cgagatcgag gtgatgccgc gctcgacgag gaagtgcgcg aattccgggt aatccgaagg
     1141 agcctgcccg cagatgccga tcttcttgcc ggcgcggtgc gcggcgtcta tggcctgcgc
     1201 gatcatgagc tggactgcgc cattgcgctc gtcgaagagg tgagccacca tttcggagtc
     1261 acggtcgagg ccgagggcga gttgggtgag atcgttcgag ccgatggagt agccgtcgaa
     1321 gatgcgcagg aactcgtctg cgaagaccac gttggcgggc aactcgcaca tcgcgtaaat
     1381 ctccaactcc cgctcaccct gtttgaggcc atactcgcgc atcaggttga tgacgcgctc
     1441 gccctcctcc accgtgcggc agaagggaat catagccttc acgttaacca gtcccatgtc
     1501 ctcgcgcacg cgcctgagcg cgcgacattc gagatgaaac ccctcgcggt aacgttcgtc
     1561 gtaatagcgc gacgcgccgc gaaacccaag catcgggttc tcttcaaccg gctcaaactg
     1621 ctggccgccg atgagccgcg cgtactcgtt cgatttgaaa tcggacatgc gcacaacgac
     1681 cggcttcggg tagaaggcgg aggcgatgcg cccgatgccc tcggcgaggc agcgcacgaa
     1741 gaactcgcgc gggtcttctt ctccgatgcg ttcttcgatc tcgcgtatat cctcttcgcg
     1801 tgcgatgtgc gggtaattga cgagcgccat cggatgaatg ccgatgtgat tattgatgat
     1861 gaattcgagg cgtgcgagtc cgacgccgtc gttcggcaga ccggcgagcg cgtaggcgtg
     1921 gtcagggtcg ccgacgttca tcatcacctg cgtgcgcggt cgtccggcat cgctgacggt
     1981 gtgcttgtcg atcacgaaat cgatcaggcc gtcgtagatg tttccctgcg cgccctcggc
     2041 gcaggagact gtgaccggca tgccgttcgg gagcttacgc gtgccgtcgc ccgtgccgac
     2101 gatgcagggc agaccgagtt cacggctgat gatggcgctg tggcaggtgc gcccgccccg
     2161 gtccgtgacg atagccgcag cccgtttcat gatcggctcc caagcgggat cggtcatgga
     2221 ggtgacgagc acgtcgcccg cactgaattc ggcgagatga tcggggtcgg ccagcacatg
     2281 gacgcgcccg tggccgatct tttcgcctac ggcctgtccc gtgacgagcg gctcctgatg
     2341 gctgccggtc agacggtagt tgagcacgaa gttttctcga ttggtcgaat gaaccgtctc
     2401 ggggcgcgct tgcaggatga agagttcacc ggtgatgccg tctttcgccc actcgatgtc
     2461 catcggttga tcgtgtcccg cacgttccga atagtgtgtt tcgatgaggc aagcccagcg
     2521 cgcgagttgc agcacctcat agtcttcgag cgagaagcgc cgacgctgcg ccgccacgac
     2581 ttcgcgaatc cgcgtcccgc tcccgtcagg accataggac atcttgactt ccttgctgcc
     2641 gagacggcga ctgacgaccg cacgcgcccc ctcatgcaga gtgggcttga agacgaccca
     2701 ctcatccggc gtcgccatcc cctgcacgat ggcttcgccc aatccccacg ccccgttgat
     2761 cgtaaccaca tcgcgaaagc ccgattcggt gtcgagcgta aacatcacac cggaggtggc
     2821 gaggtctgaa cgcaccatcg gttggacgcc gatggagagc gccacctcga agtgatcgaa
     2881 gctgcgagcg gtgcgataag agatggcgcg atcagtccac agagaggcga agcattgctg
     2941 gcacgcggca aggagttccg cttcgccgcg cacgttcagg atcgtgtcct gctgcccggc
     3001 gaacgaagcg tccggcaaat cttcggcagt cgccgacgag cggacggcga cttcgcaggc
     3061 ggagcgtgtg cgttggcaaa ggcgacgata agcgtcgaga atcgcttcgc gcacgtcgta
     3121 cggcaagggc gtctcaagca tgagggcgcg agcctctcgc ccggcggcgc tcaaggctgc
     3181 gaggtcttcg cagtccagcc cgtcgagcag tttgcgcagg cgcgtctcca gattgttcgt
     3241 cgcgagcaag tgacgatacg cttcgctggt cgtggcgaaa ccgtccacgg cacgcacgcc
     3301 tttcggcacg agcgcgcgaa acagttcgcc cagactcgcg cacttccccc cgacgagaga
     3361 aatatcatcc gcgcctactt ccgacagatc gagaactaaa tcttttatca ggtttttcat
     3421 tgagcacatc cctcgcgcgc ctggcacagt gaatagaagt aaaacttccg ctgccatcag
     3481 taacttgcca gcggtcaact caggttggat gaggccgggt cagacgcccg cacgcgaaga
     3541 caccgcagga caccggttgg caccggccaa ccagcatctt cgtaaagatt gtatccagta
     3601 gaaaacagac tatacgccga gggcgcagtc gtctgtacgt ttgcgcacgg gtgtcaggca
     3661 ggtcgtaacg ctatcgcgcg cccgcgttca acttccgaca gacatagttg agcgggcaca
     3721 cctcacgtgc gccaaacctc tcacgcctga tccaaaccgc ttacactttg gaggatgttg
     3781 aggcaaggcc gcccgtcatt aggccgacga cgcgccacgc cccatgatga tcgcctttcg
     3841 cctcttccat actgagaggt gccttgagca gtggatcgta gatgacgttg cctgcaacga
     3901 cggcgtagtg acgcgcccag taatgtgcca tctccgggcg gtcgatacag acgatggccg
     3961 gtccttcggg gaagtcgaaa tctttcagcg tggtttcgcc gtcgaactcg gccagcgacc
     4021 agtcacggcc tgttatcttg agcagggcgt gcaacatctc tccggaccac agaccgtgca
     4081 cgaagcagtc cggggcaacc gattcaagta cgcgctcgta ggccacgccc gccgccatcg
     4141 caatcgtcgc tacaccgcag tcagcccacc gcctttgtct gatgtgaaac atcttccgca
     4201 tcattctcct ttcatctttt catgctcacg tcgcggcgca cacgactcca tagggcgtcg
     4261 tcatcaacaa ctcgccggcg ctgagcggat tcatattttc cgtaatcatt taccaccctt
     4321 cggacgcgat ttcaatgaga ctgaggatcg cgcccgcaca ttacagcgct acggcattgc
     4381 tacggctgga atcggcgacg acataactga cggccaacgt cagctcgaag tcgtggacgg
     4441 atacggcgtg aagagatgca aagtttacgc cgatgttgaa gctccggcgc aggaggttat
     4501 gattggataa ccgtcatact gtgacgtaaa accctgctct ccttctacgg ggtcatcgtc
     4561 aggcgacgct tgccactcgc actggcatcc ggcgcgctgg cggcgttcgt tgttcccctc
     4621 cggcactttc gcgcttgacg cacggctatg ccgggaggag cacgcggatc gaagttaact
     4681 gtcagaagat aaagatttga tagacggttg cgaagtctgg cgggatgaaa tgagagggat
     4741 tttcgtgagg ccgcgagacg gaaacggttt ccgtcaaact ttgccgcgca cggtgcaacg
     4801 cttcgttctt acctctcgaa gagggcgcgg agttgtccgc ccatctcttc gggaaacccg
     4861 ccgtgccgcc gcctgagaaa atcgttgacc cactccgcca cggacgaggc gagttcgcgt
     4921 tcctgcgtcc ggcgcaattc agtttcagcc tccgtctcct gtttcgtcgt ctccattttc
     4981 tgctgcgctc gctgctcgcg ttcttctcgc ttgataactc tgatcccgtt tttcgctacg
     5041 ttcattttca acaccctgct tgtattatgt tccgggttct aaagtactca ctcaggcact
     5101 ctgtcgcgct gtgctccatc ggttagccgg agcttctgcg ccttcgacct gcccggacga
     5161 taagttagtc ttccaacttg ccgtgcattc gttgacgtac ctcttgagtg cttcatcgag
     5221 tgaaggcagg agcacgcccc tctcgctgcc cagcacgcta tacatcggac gccgcgccgt
     5281 cgtgccgtcg agcgcacgtg tcgcgcagcc ctgcaccccg ctggcatcga gtccggccaa
     5341 ctgcgcggcg cggcgcgcta actccgccca cgtgactgcg ccggagtttg ccagatgcca
     5401 gatgccgcgc tcgccgtcga tcaacagatc gaggctggcg ttcaccaagt ccggcacgta
     5461 ggtgggcgac acaatggcgt cgtcggctgc cttaaaagtc tcgccggagg cgagcgtgcg
     5521 caacgcggcg gtcacgaagt tatgctcgtc ccaggggctg aagaatgcgc tcgtgcgaac
     5581 aaccagcgcc gctggatgaa cttccagaat gcgcctctcg gcctcggctt tgctctgccc
     5641 gtaaacattg agagggacgg tgcgcccgct ttccagataa ggctccgaca cctcgccgtc
     5701 aaagacgagg tctgacgaaa atgtcaggag cgatattccg cgttcggcgc aggcgacggc
     5761 cagggttgca gccccgtccc gattctctcg aaagcaggct tccggctcgt gctccgcttg
     5821 atcgacgcgc acgtaaccgg cggcgttgac gatagcccac ggatcatatt cgtttaatgc
     5881 cgcgcgcacg gactcctgat cggcgatgtc catctggcaa cgagaaagga gacgatagga
     5941 gatgcctcgc acttcgcaca gacgcgcgaa tgaacttccc agcgttccgg tggctccggc
     6001 gatgattaac gggcgggtcg aactcttccg cgaactcgca cccctgaccg gttgttttgc
     6061 cgtagccgcc gcgcgcggtt ctctgcgcac cggcgtatag agaagccgtt cgagcctctg
     6121 ccaccagccc ggcccttcga tggccgggtg attaaactcg cgtcccgcgg ccagttcgcg
     6181 caacagatgt gcgatggcgg tggggcgacg atgcgtgccc cgcacgtcga acacgcccgg
     6241 ctcgtagaag cccgcggagc aggtcaacaa cttgttccag tcgaatgcgc ccagcagcga
     6301 ccacgcggtg acggcgcgca cgtccacgcc ctcttcacgc aaatcctgac acgtctccca
     6361 aacttccttg aaccagcgaa gctgctcttc gcgcgtgcag ccgagatgaa cctctgtgac
     6421 ggcgatgggc agcccgtaac gctcccacgt ctcgcgtaac agcgtgcggt gtccggccag
     6481 cccttcggca cacaccctga ccgcttccac gtccgcataa cgatctttca tgttcccgcc
     6541 gtgcgagcaa gcggggtagc gttccagacg ctcgtcgagg aaacgctcgc tcgtcagata
     6601 gtgattgatg cctacaacgt ccggcgggca ggggttgtct gtgaaccagt cgagttcggc
     6661 ctcgtcaatg ccgaccccgg tgaaataagt ccacatcggg tgatggcgat tgaggcgacc
     6721 cgtcagcagt tcaaaggtga gccagcgtcg ctcgttctca aggtctgcct gatagcggag
     6781 tttgcgtgtg ctgtaggtct tacccagatc gtccgtctga atcaactgcg ctgccggatt
     6841 gatttcgcgg atcgcgcgca tcgagagcgc aaccgcgcgg cactggatga gcaacgcctc
     6901 gcagagcgtg cgccggtctt ggccgtgcgg ataccagagt ccatatcgcc cgctgaaacg
     6961 cgcggtcgtc agaggctcgt tgacgggcgt gtaagcatcc acccacgggt aacgctcggc
     7021 gacgccgcgc gcatactcgg ccagcttttc gggaaaggcc ggatcgacca gactcgtgtg
     7081 gcgcggcccg ctgccgtgat gcacgaggcc gacgatgggg cggatgccga gttcgcgcag
     7141 gcgtgcgagg cgcgagtcag cccaagacca gtcagcggtt tccagaccat cgggcgcggt
     7201 gcgttcccat aacacgggat agcgtatggc gcggattccg agtccggcaa agaggtctag
     7261 gtcttcgatt cgttcggcgt ggccgttgcg ctcaagttga tcgaaatact catcgcccac
     7321 gcggttgacg gtgcactcga ctccgcccca tagttcgatc cgtgcgttgc ctgttgtctc
     7381 tacgcccatg ttcgttctca caccttcctg tcgccgctct cagcttgagc gacggtttaa
     7441 agttcacagc gaacccgtct cttttcatag cccgcgcgtc taactcgcga cggagaagtt
     7501 aacggctgct gactcggcac ccgcagcgcg ttgtttgaga ttgcgcgagg gcgtggcttc
     7561 cgccgtcacg cgcccgtcat caatcgcgtt ttcgatcagt tcagacatgc gcgcccaggt
     7621 catgtcccag gaagtctgcg agagaaactt atcaaccaca gcctgccgcg ccgccgcatc
     7681 ctctgtcatc acggcctccg ccgccccgat gaaagcttcg gcggtgtcgg ctatcctgac
     7741 cagacttttt tccccgtacg ggcggacgac atcacggatg gaggtcgaga tgaccggcag
     7801 accggcggcc agatattcgg gagtcttggt cgggctgatg aaacgcgtcg cgtcgttcag
     7861 tgcgaagggc atgagcgcca cgtcccagcc cgccagatat tccggcaact cgctgtaagc
     7921 tttgccgccg aggtagtgga tgttggcgcg gcgcggcaaa ctcgcctgct cgatcttgac
     7981 gaccggcccc acgatcacga actgccagtc gggtcgtgcc gccgagatgc ggtcgagcag
     8041 ttcgagatca attcgctcgt caatgactcc gaagaaaccg agccgggggt gtggaatacc
     8101 ggcttggtct gccggttcgc tcatggcgtt tctcgcctgc gcgaaatgcg cggcgtcaat
     8161 gctcgaaggg aaggcgtgaa tgtgggggtg tttcgggtgc tgcttgcgct tcgactcgta
     8221 gaggctctgc ccgccagtaa agacgaggtc ggcgcgacgg aacaactctc cttcacagaa
     8281 tctcaacgcg ggcggcgcgt ccttaaagcc ggagagttcg tccatgcaat catagatgac
     8341 ggcgcgcggg cgcaggtgtt gggtgaaagc catcgccatc ggcgtgtagt accagaggag
     8401 gtaatcgtcg atcttctgtt cgacgaagaa acggtcgagc aaatcctgca aggcggcatc
     8461 tctggccgga tcatcctgta gtcgttcggg cagccacgga accatcacga cagtcccgca
     8521 ctcgcgttcg ctgacgtgca tccgcaccgg cccgttgtca taaaccggct cctcgatgaa
     8581 gaagacgcga cgcgctttcg cacaccggct caacaaatgc tgcggccttt gatagacgaa
     8641 atcccaacgc agatgcgaga ggcacacgag gtcgggcgcg gactcgaagc cgcgcgcatc
     8701 cggctctgcg gcgagagcaa aaccgtcatc ggttgagcgt gaacgggcgg gcagggaacg
     8761 cgcacccgca tgccgtccgt tggaagacgc caccttgagg ccgttcatgt ccgaagttgc
     8821 tgccgcgccc cggcgcagtc catcctggtt gtcgtatcgc atcccaccgt ccatcttctt
     8881 tgaatctttc atgtgtgcct ttcctctcaa atacaacttg cgtgcgtcgc gtgtggaaag
     8941 ccgtgcacaa taatcgggcg cgggtaagtc aacgcacttc cgcgcccggc tcgtgcatat
     9001 acacgcgaaa cacaacttaa tgacattgcc cgtcgcggtc acaccagaag gttaacgctc
     9061 gcagcattgc cacacttaat caggggtagg aaccaagata tacgagggcg ggaagtctgt
     9121 ctgtacaata tcgaacagca gatgtggctc ggtaaatttc gagtcactac agtctgaatg
     9181 gccgactgat ctctctgtgc tcttccgccg ctggagactt gtcgaggagt tcgtcaccgg
     9241 ctaccggata ttcatggcgg gcgatctcgt ccagatagtc tagaaattgt tgaaagagaa
     9301 tttcgctctc gccatccgag aagttgtcgt tcgacttctt tgtcacgtag ccgccgaggt
     9361 taagtcggaa ccgggggtcg cggagcagca tttgcgccag aatatccgag acgacgaaac
     9421 ggaaatgagt gggcgcgggc gtggcgtcgg cgtcgctctg ccgctcgaac tcccccgata
     9481 tgctgagagc cttctgcgcg gcgggtgaaa gctcggatgt gtcgccttcg ttcgtcgtct
     9541 ccagactgct cttgctcgga tagacaactc tcaacgcgct cttggacgga tcgtaattcg
     9601 gtgaagcgtt gacgacgccg agtgtggccg ggagaaagag cgggtcgccc gtgcgcgtgt
     9661 tgagtcgctt gtagatgtgt ggccggaaga gtcgtccggc gttacaggcc gtgatgatcg
     9721 agaggcggat gcccgtgcct tcgagtctct cttgcagcgc gccgaccgag agataggagc
     9781 gggcgtcttt cgcggcgtgc ccggcctgta gctttagccc gtgcccgttc gtgccgtgcg
     9841 attcgagaat cagcacctcg acggggttgt ccggctgcga ttgattatgc gtccgaatct
     9901 gccccagcac gacgcgccag tccgcgtcct cgaagtaggt gaagccgagc cgctcgtaaa
     9961 acttccggtt cgtcggcacg ctcaaatcag gactgaacac gatccctttg ccgcgcccta
    10021 tcccggatag acgcacgcga ctgacgccct ccggccagac ttcagataag atggccgccg
    10081 tcgtctccgc acgcgccgcg tctgccagtc tcctcgccgc acctcgcgca ctcgcggcgg
    10141 gaaacgaccc ggagttgcgg ccttgcgccg ccgtggggaa attcccgcag gggagcagcc
    10201 cgacgagcag ggagagcaga aaagttctct cgattcctct cacaacaaac ccgtgctcca
    10261 ttctttcgcc ccgtgatgaa aaaagccccg catccctttc gttagtcgag acggctcgcg
    10321 ggcatgcggt tcacaaccga agcgtatatt tgcaggtacg cctgcgtctc gcgcgggatg
    10381 agcgtgcgcc atgattcccc gccgcgcgcg agatagcctg ccagccgcgc cgggttcgag
    10441 ttgtacccgg cggcgagcag ttccacctgc gtcgcggtgc gagtgttcaa agcgttgacg
    10501 acggcttcct ccgcgatgag atcgttccag gtatcgtgca tgtagaggag catggcttcg
    10561 agggcgttcg agtggtcgcg catgccgctg gcgaaatcgg gattgagtcg cgcttccggg
    10621 tggcgctccc tgaccatccg gtaggtcgaa gggatcatct gcaccatacc gcccgccccg
    10681 gctgaactca ccgaatagcg gtaggtgtct cccgcgttga gggcgtagag agaagcgatc
    10741 tcggcaaaca acgccgcttg atcctcagtc cggaaacggt cgtggtcggc atgctcgacg
    10801 atgcagagac gttccgccac gtcaataatg tccggcgcga cgagcacacc cgttgcggca
    10861 agacgacccg ccgcctcgtc cagcatacgt cgcacgtagg ctctgccttc gcgcgccaac
    10921 tctgttgatt cgagcgcggg atgtaccgag gtgtagtaag cgctgtgcgt tgttccgccc
    10981 tgcttgatcg ggtagcggat tagcagagga acgtgcgcgc gcccaccggc gtcggaaatt
    11041 gtgacggcgg tgttaacgcc gttagcgcgc acgattctga cgtggacaag atgcccgcgc
    11101 gaacttttca cagacgcttc cgcctctctc cgacggaaga actctttagg cagcgtcaac
    11161 aggtttagcc cgtctgtcgc tccatcttca aacgcgacgt ggacggcatc agccgggggc
    11221 gcgggctgca ccgcaagaag ttttttggcg acccggattt gtgcatgaag cctgtcgagt
    11281 ctttcctgtt cggggcgacg tgccgtagag atcgttgaac tcacttcgcg tggtgcgagc
    11341 gtgagggagg gtcgcgactg tggggtgtgc gtgaggagcg gaaatagcaa ggctacgccc
    11401 ggcagcacgg aaacgaatgc ttttttcatt acttcctaaa cgttcgactg tagagattga
    11461 gtttgggggt aaggcgcaaa tactacggtg cggcgtggcg gatgtctgtt cgctttcgca
    11521 cgaaatcagt tttttttatc gtgcgcccgg tttgattcaa ctctgtgcga tcaacggcat
    11581 t
//
LOCUS       DASWNS010000285         4482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_165919, whole genome shotgun sequence.
ACCESSION   DASWNS010000285 DASWNS010000000
VERSION     DASWNS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165919"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VGB76_10085"
     CDS             complement(<1..881)
                     /locus_tag="VGB76_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099286.1"
                     /translation="MNSDGSGLVNLTNHQAGDYEPAFSPDGSRIVFNSTRDGGDGEIY
                     VMNADGTNQTRLTTNGGTSPSFSPDGTKITFTTASSSGNGNAVALMNADGTNPVALTN
                     NAASDEEPAFSPDGAKIVFTSARDGNSEIYVMNTEGSSPARLTSNAGFDFAPVWQTLL
                     SCAPPLPNGVAWYRAEDNAADEFGANDGTFQNGATTASGFAGSAFGFDGVDDVIDIGQ
                     LTDLQNAQQLTVMAWVKKRDLNNTIAGIIGKWNGCTGCTDDTFLLYNSESVTVNKGGF
                     VVRFTDDTAAAIAGTTD"
     gene            complement(1237..1641)
                     /locus_tag="VGB76_10090"
     CDS             complement(1237..1641)
                     /locus_tag="VGB76_10090"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0099287.1"
                     /translation="MGKTSKAFDMERLFKALADRTRLRLLNLIGSDEVCVCFFVEVLG
                     ESQPKISRHLAYLRRAGVVAARREGKWMHYSIAAPADEHAARLFGEVRAWLAEDREMQ
                     RDRERLIKVCCAPSQPVQLQGAPRPASLAAQG"
     gene            1765..2301
                     /locus_tag="VGB76_10095"
     CDS             1765..2301
                     /locus_tag="VGB76_10095"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEY0099288.1"
                     /translation="MEKQTMRTAQALKAHLALNVRSVERSVEFYRKMLGLEPAKLRSG
                     YAKFDVENPPLNLTLNEVDFAERGALSHLGIQLATTEDVLSMRRNWLDAGLETRDEMQ
                     TNCCYALQNKTWVQDPDGNEWEAFVVLEDNLPVTSSCCGVSETGTTGEAATNATTAET
                     TAEASSCCATAAPLTISR"
     gene            2331..3083
                     /locus_tag="VGB76_10100"
     CDS             2331..3083
                     /locus_tag="VGB76_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY0099289.1"
                     /translation="MSNQKGGNVQPTLARRVVAEAIGTALLLAAVVGSGIMGERLAGG
                     NVALALLANTTAIGAALVALILTFGAISGAHFNPAVTLATASQGGLRWRRDVPAYLAA
                     QTAGAFAGVAAAHLMFGEPLFAASRHAREGGAQMFSEALATFGLLSVIWGCARLRSSA
                     VLMPFAVAAYIMSAAWFTSSTSFANPAVTLARAATDTFTGIRPQGAPAFIAAQLAGAA
                     AATALFRWLAPGLPADAADVVLPHDESETRHG"
     gene            3114..3536
                     /locus_tag="VGB76_10105"
     CDS             3114..3536
                     /locus_tag="VGB76_10105"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY0099290.1"
                     /translation="METKRERVLILCTGNSARSQLAEGLLRHDAGDSFEVFSAGVSPS
                     RVRPEAIEAMREIGVDISGQRSKAVEEFAGQRFDYVVTVCDNAREQCPVFPSETRRIH
                     WSFDDPAAASGGEAERLAVFRRVRDEIRERLREFVASS"
     gene            3727..>4482
                     /locus_tag="VGB76_10110"
     CDS             3727..>4482
                     /locus_tag="VGB76_10110"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEY0099291.1"
                     /translation="MIKLPEPQQQGSRLTLPTRRIALFILCLVVSALSGTGAQERRKS
                     DAPNLFPVKQDGKWGYIDRTGKMVVAPQYDVAWEFREGLAYVKAGAQRGLIDRNGKLV
                     VNLQQVDLAGLFSEGLAPVQTASQPPRFGFIDRTGKLVINPQYDAARGFEEGLALVMV
                     ERKYGFIDKRGRMVVKPQFDKAASFSEGLAAVVVDNKHGYIDRTGKLVIAPQYNNAYN
                     FAEGLAAVAIGGRYACINRKGEVLIEPQAGYVGA"
BASE COUNT          837 a         1351 c         1458 g          836 t
ORIGIN      
        1 atgtcggttg tgcccgcgat ggcggcggcg gtgtcgtccg tgaagcgcac gacgaagccg
       61 cctttgttca ccgtgacgct ctcgctgttg tagaggagga aggtatcgtc cgtgcagccg
      121 gtgcagccgt tccacttgcc gatgatcccg gcgatggtgt tgttgaggtc gcgctttttc
      181 acccacgcca tcacagtgag ttgctgcgcg ttctgcaagt cggtcaactg cccgatgtcg
      241 atcacgtcgt ccacgccgtc gaacccgaac gccgacccgg caaatccgct cgcggtcgtc
      301 gcgccgttct ggaacgtgcc gtcgttcgcg ccgaactcgt cggcggcgtt gtcctctgct
      361 cgataccacg cgacgccgtt cggcagcggc ggcgcgcagg agagaagcgt ctgccagacg
      421 ggcgcaaagt cgaagcccgc gttgctggtg agacgcgcgg gcgacgagcc ttcggtgttc
      481 atcacgtaaa tctcgctgtt gccgtcgcgg gcggacgtga acacaatctt cgcgccgtca
      541 ggactgaaag ccggttcttc gtcggacgcc gcgttgttag tgagcgcaac cggatttgtc
      601 ccgtcggcat tcatgagagc gaccgcgttg ccgttgccgc tgctgctggc ggtcgtaaag
      661 gtgatcttgg tgccgtcggg gctgaacgag ggagaggtgc ctccgttggt ggtgaggcgc
      721 gtctggttcg tcccgtcggc gttcatcaca taaatttcgc cgtcgccacc gtcgcgggtc
      781 gagttgaaga cgatcctgct gccgtcgggg ctaaaggccg gttcatagtc gcccgcctga
      841 tggttggtga gattgacgag tccagacccg tcggagttca tcacatgaat ctccgggttg
      901 ccgctgcgtt cggacgtaaa aacgatcttc caagcctacc gcacacgccg ccagcgtcaa
      961 gaccaacccc cagacgagcg ggcggcggag acgctttgcg cgacctgttt tcggtgaaaa
     1021 ggatgccggg ggaaatttca tattgatctt tcaaaggaag gtttctgcca gtagttaccc
     1081 gacgaggcag cggcggcaac ttaggtcagg aacgacgaag gtgtcaagaa aaacgcgcgt
     1141 ccgaattaag tcccaagtcg caagccagcc gcgcttgacc tgaggcttga gacttgagac
     1201 ttcggactta acacttagga cttaacactt aggacttcat ccctgcgccg cgagactcgc
     1261 ggggcgtggc gcgccctgca attgcacggg ctggctcgga gcgcagcaga ctttaatcaa
     1321 ccgctcgcgg tcgcgctgca tctctcggtc ttccgcaagc cacgcgcgca cttcgccgaa
     1381 gagccgcgcc gcgtgctcgt ctgcgggcgc ggcgatgctg tagtgcatcc acttgccttc
     1441 cctgcgggcc gcgaccacgc ccgcacgacg caggtatgcc aggtggcgcg agattttcgg
     1501 ctggctctca cccaagacct ctacgaagaa acagacgcac acttcgtccg agccgatcaa
     1561 gttcaacaga cgcagccgcg tgcgatccgc caaagcttta aagagcctct ccatgtcaaa
     1621 cgccttgctc gtcttcccca tgccaatgca cccctccgaa aaaaaaatta caaatacgtc
     1681 ttgacatata tgtccaccgc gcgtatgctg cgcacatatt cgccgatgcg aatataacat
     1741 gaccagaaag tgggaggcaa aaaaatggag aagcagacaa tgaggacggc gcaggcgttg
     1801 aaggcgcatc tcgcgctgaa cgtgcgcagc gtcgagcgga gcgtggagtt ttaccggaag
     1861 atgctcggac tcgaaccggc gaagctgcgt tccggttatg ccaagttcga cgttgagaac
     1921 ccgccgctta atttaacctt gaacgaagtg gacttcgcgg agcgcggcgc gctctcgcat
     1981 ctcggcattc aactggcgac gaccgaagac gtgctctcaa tgcgtcgcaa ctggttggac
     2041 gcagggcttg agacgcgcga cgagatgcag accaactgct gctacgcgct acagaacaag
     2101 acgtgggtgc aagacccgga cggcaacgag tgggaagcct tcgtcgtgct cgaagacaac
     2161 ctgcccgtca cgtcatcgtg ctgcggcgta tcggaaaccg gcacgacggg cgaggcagcg
     2221 acgaacgcga ccaccgcaga gacgacggcg gaagcctcct cgtgctgcgc gacggctgcg
     2281 cctttgacga tctcgcgttg aacttcgtgc aaggaaagga cgcacggcgt ttgtcgaatc
     2341 aaaagggcgg aaacgttcag ccgacgttgg cgcggcgcgt ggtggccgaa gcgattggca
     2401 cggcgttgct gctcgcggca gtggtcggct caggcatcat gggcgaacgg ctcgcgggcg
     2461 gcaacgtcgc cctcgcgctg ctcgccaaca ccaccgcgat tggagccgca ctcgtcgcgc
     2521 tcatactgac tttcggtgcc atctccggcg cacacttcaa tccggcggtc acgctcgcca
     2581 cggcctcgca gggcggactc cgctggcgac gtgacgtgcc cgcctatctc gcggcgcaaa
     2641 cggcgggagc gttcgcgggc gtggcggcgg cgcacttgat gtttggggag cctctgttcg
     2701 cggcgtcgcg ccatgcgcgc gagggcggcg cgcagatgtt cagcgaggcg ttggcgacct
     2761 ttggcctgtt gtccgtcatc tggggctgtg cgcgcctgcg ctcgtcggcg gtgctcatgc
     2821 cgttcgccgt cgccgcttat atcatgtcgg cggcatggtt cacgtcttcc acctcgtttg
     2881 cgaatccggc ggtcacgctc gcgcgtgcgg cgaccgacac gttcacaggg attcgcccac
     2941 agggcgcgcc cgccttcatc gcggcgcaac tcgcaggcgc ggcggcggcc accgcgctct
     3001 ttcgctggct cgcgccgggg cttccggcgg acgccgccga tgtcgtcttg ccgcacgatg
     3061 aatcggagac gagacacgga tgagcgaaga cgaacaggtg aaggagatat tgaatggaga
     3121 cgaaacgtga gcgcgtcctg attttgtgta cgggcaactc tgcgcgcagc cagttggcgg
     3181 aagggttgct gcgccacgac gccggggaca gcttcgaagt gttcagcgcg ggcgtgagtc
     3241 cgagccgggt caggccggaa gcgattgagg ccatgcgcga gatcggcgtc gatatttcag
     3301 ggcaacgctc gaaggcggtc gaagagtttg cggggcagcg gttcgattac gtcgtcacgg
     3361 tctgcgacaa cgcgcgcgag cagtgcccgg tctttccttc ggagacgcgg cgcatccatt
     3421 ggagtttcga cgatccggcg gcagcgtcgg gcggagaggc ggagcggctc gccgtctttc
     3481 gtcgcgtgcg cgatgagata cgcgagcggc tccgcgagtt cgtagcgagt tcgtagcgag
     3541 cgcatagccg gttcgcagca agttcgcctt gacgcgcggc tgacggcgat aatatcttac
     3601 gccttgaagg ctgaattaaa gggtcggatg tcgcctcgtc gaaactctcc cgcacgcgcg
     3661 cacgtcgctt ccggtcgcca cccgcgacga tgcttacaca gttgtcaatt gttcgcttca
     3721 aacgttttga tcaaacttcc agagccgcag cagcaaggat cacgactcac tctgccgacc
     3781 cggcgcatcg cacttttcat tctctgtctc gtcgtgtcgg ccttgagcgg cacgggcgcg
     3841 caggagcgca ggaagagcga cgcgccgaat ctgtttcccg tcaagcaaga cggcaagtgg
     3901 ggctacattg atcggacggg caagatggtg gtcgcgccgc agtacgacgt ggcgtgggag
     3961 ttcagggaag ggctggccta cgtgaaagcg ggcgcgcagc gcggcttgat cgacaggaac
     4021 ggcaaactcg tcgtcaacct gcaacaggtt gatctcgctg gactcttctc cgaaggactc
     4081 gcgcccgtgc agacggcgag ccagccgccg cgtttcggct tcatagatcg gacgggcaag
     4141 ctcgtcatca acccgcagta cgacgcggcg cgcggttttg aagaaggact cgcgctcgtc
     4201 atggtcgaac gcaagtacgg cttcatagac aagcgcgggc ggatggtcgt caaaccgcag
     4261 ttcgacaagg ccgcgagttt ttccgaaggg ctggcggcgg tcgtcgtcga taacaagcac
     4321 ggctacattg atcgcaccgg aaagctcgtc atcgcgccgc agtacaataa cgcttacaac
     4381 tttgccgaag ggctggcggc ggtcgccatc ggaggccgct acgcgtgcat caaccggaag
     4441 ggcgaggtgc tgatcgagcc gcaggccgga tacgtcggcg cg
//
LOCUS       DASWNS010000286        17238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_115978, whole genome shotgun sequence.
ACCESSION   DASWNS010000286 DASWNS010000000
VERSION     DASWNS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17238
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115978"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VGB76_10115"
     CDS             complement(<1..296)
                     /locus_tag="VGB76_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099292.1"
                     /translation="MPEQLSNSNFTRTRSARFARRLVLSASLCFGVSATCAATPASAA
                     ALFAQDAAKPQAKVAPPATTPAVQEETRKDAKVKTRGAQEKKHASSPASEITRAR"
     gene            complement(388..1599)
                     /locus_tag="VGB76_10120"
     CDS             complement(388..1599)
                     /locus_tag="VGB76_10120"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY0099293.1"
                     /translation="MNDERGTRNARTKRVALRSTLRAARSTFYVPHPTSSVHRSSFIL
                     HRFVVLLSLLVAPAIASAAGAWESQTSGTLAWLRAVYFVDERTGWAVGSKGALLRTTD
                     GGASWEVRRRPTEDTLRDVYFTNEETGWLVCDRSIYLLKTKAEPRSYLMKTSDGGESW
                     ARVEVTGADVDVLLTRVAFVEEMRGWAFGEMGALYNTTDGGATWARQLVPTKHLLLGG
                     AFLNEGQGWLVGAGNTILYNPDGEGWRAGSSPISTATRFNAVAFVDERRGWAVGERGL
                     MLVTTNGGRAWFPQESNVRVELSDVKFLDARTGWAVGAEGTIIHTTDGGAHWQIEPSG
                     TRHPLERLSIASRARAWAVGFGGTIVRYADDAQATPPPQLKLPGNEQPKGQPKERPRR
                     VNVNSATRREH"
     gene            complement(1606..2574)
                     /locus_tag="VGB76_10125"
     CDS             complement(1606..2574)
                     /locus_tag="VGB76_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0099294.1"
                     /translation="MKVHTMKLATAAFTFFLLTGGSGALAAQEAQEGARPSAPAARKP
                     SLSQTVQYFRVFGEDDTMQEDKKKSEQKTRPRTATNASGTSKGKAGERLEPDDLSAPP
                     REDMSAEMLANRRESLSEEEAAVVPYYNNFMATYRLGPEDVISITVFNLERYSKAGII
                     VPPDAVISHPLIPEGVFVGGKTTKQVAEEITKRLDEYVIDPKVTVSLDKAQSAVFWVV
                     GDVAQPGIRPMSRRLSVTEALALSGGVLDTGNKKKVMVMRRQANGFLQPTIIDVAAIE
                     KGRMPDNYFLNPGDQVIVPGNKLKTFDRVMKLLPVVSFFRIFTGGF"
     gene            complement(2621..4012)
                     /locus_tag="VGB76_10130"
     CDS             complement(2621..4012)
                     /locus_tag="VGB76_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEY0099295.1"
                     /translation="MSPTRRFNARTPLLILAESLLMFGGMVAAAHIRLGSHDAYYELV
                     GRNGFYKAALATAFCLGAFYLYDLYDFVVMHDRRELVLRLIQALGLAWIALAIVFYLL
                     PQLMLGRGISLIALPLALALMVAWRISIHWLLGHPMVGERILIVGSGTAAIEIAREVL
                     ERRDAGYRIVGFVDNDPALVGKSIINPRVIGVTTDMAQIVKRENIDRIIVAQGERRGQ
                     FPTRQLLDLSLGGHVAIEEGASFYERTTGRVHLNMMRPSWLIFSGRGRQARLSGVARA
                     FLHRGVAFVGAIVSLPIALLTAVLIKLESPGPALYRQERVGQNGRPFTIMKFRSMRVD
                     AERNGPVWAQTGDDRTTRVGRVIRKIRVDEIPQFWNILKGEMNFVGPRPERPHFVAQL
                     AEEIPFYEQRHLIPPGLTGWAQIKYPYGSSIEDARQKLQYDLYYIKNQSLVLDAVILF
                     ETVKTILFGRGAR"
     gene            complement(4178..5296)
                     /locus_tag="VGB76_10135"
     CDS             complement(4178..5296)
                     /locus_tag="VGB76_10135"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEY0099296.1"
                     /translation="MGRVYEALKRAAENGSADQKSKDETTARRASTAAAVVEDEEVTL
                     LTEASAPANGNSNGDHHGGNHAPSAAVPEVVLRRTSALFNAPENRAHVSASAEHIDAA
                     TAGSALPGGQASRVAGATADAVSSARALEFPSQEISHARVEPHLIAITDPRSPHAEQY
                     RALRTRILHAGERRRVQAIVVSSAGVMEGKTVTALNLAWLLAQTDGVRALLIDGDLRN
                     PCAADYLGIDAPAGLSEVLAGEATLADTIIRLEPAGLHLLPGGTVRDNVAEILSGPKF
                     STLLGELRRMFDYIIIDAPPLGIFTDATVLIGRADGALMVVRSGKTRYAALDRVLEPL
                     PRERLLGIILNGADDNKDDSSSYYQRRYYRKETVTKEQ"
     gene            complement(5296..6387)
                     /locus_tag="VGB76_10140"
     CDS             complement(5296..6387)
                     /locus_tag="VGB76_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0099297.1"
                     /translation="MYKEFYGLKELPFALTPDPRFIYFTPSHTEAMANLHYGIESGKG
                     LIVVTGEVGTGKTTILRWMMQRLDRTVLVGYIFNPRLTVPEFYQHVARLFDVQEWENK
                     SELLFALGQTLEARHARGLRTVLIIDEAHGLSTAVLEEIRLLSNFESDSAKQLQIVLT
                     GQPELREVLNNPDLRQLKQRVALRCEIKPLPNIEETERYVKARLLVAGAARTDIFSPG
                     AIDYIFRCAAGIPRQINNLCDNALLTGYAAGQHTIPRAVIEEVADTFDMLPDSRRGGG
                     SSRLPDEERDLPARIFNAQGRAELWSAGSAQASAVDEVIATSDTNSSAHTRAEDEELE
                     ERASTAARPSPDVNDIGAAFRRWDVMGDR"
     gene            complement(6484..8103)
                     /locus_tag="VGB76_10145"
     CDS             complement(6484..8103)
                     /locus_tag="VGB76_10145"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEY0099298.1"
                     /translation="MSVEFRQRKPGEYGQILWKRKWLIILPAIVISAAVAIVVWRLPN
                     VYQSSTLLTVRPSNIMQGIVPQLSDDDLTIRINNIGQEVFSRSSLEPLITTYNLYAAE
                     RLRGEPMEMLVERMAKKDTRIEVNKSRNDITNGFILTFKGADPYITQKVTGDLASKYV
                     NAQIKQQSQGAVETAKFLQEQLDVAKAEVDAVDKRRLETLQSNINNLPTQATSLVTNL
                     SGLYEQQKAFINEIGRLRDQKTLLNTTLGDIEKQYQIEKEDVVETLTDPKTTPAWAEL
                     SRRESQLDSELQNLLTVLKPKNPDVVAKRQELDAVKRSKQELTDDWKNRIAEKTKRVE
                     RRVDPRSYTIKANLQAIEGEVARQEKLLAQTGAQIASVQSQLSRVPQAEVALGSLDRE
                     YQTKKAAYDELLLKKGKSDLNVAATNSAQGETIQVIDPASLPVRPIAPQRPMLIALGL
                     ALGLGFGLAFAAAFEVPRLLTIQTVEDARHYTGLPVLISVPELLTPKEQRRARLRRMA
                     TAFASLTLTVLSIPALALLLRFTNVFTLFVS"
     gene            complement(8153..10615)
                     /locus_tag="VGB76_10150"
     CDS             complement(8153..10615)
                     /locus_tag="VGB76_10150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099299.1"
                     /translation="MNYKECPRSGNGRSLSSVLLAFVVAAFAFATLACSNPEKAKAEH
                     VQRGEAYLTEKKFQEASLEFRNAVQIDDRLAQAHWGLARAYEGMEQFTQAFEELRRAV
                     ELDPNNLEARVRLGNYYILAFQQTKDDKLRAEAERIAKEVLDKDPNYIEGHILRGTVL
                     FASGKREEALAALNRAVELNPQRVESLLSLALFHRQMNDPAKAEEVYKRALSINDRSA
                     LAHLEYAKFFVSQNRQDMAEVEFKRAVEVEPTNRDARRTLASFYLINKQLDRAEESFK
                     ALAELDRDRPEGRAVLADFYASVGRYDEAAGIYQDIVAKSADYARGRHRLAELMLQRG
                     DQQGAAAQVEEAIKRNASDREALILRARLRLNQDRPKDAIEDLKQVLKIEPRDQAGLY
                     FMAEACFRAGQMEQSRIFAGDLEKFYPDYLPGKLMQVQINLAAGDAANAGRLANELLA
                     RLDKAAPDARNSPQLLNELRAKTLTARATSFLQSQPSNTSAARADFIAARDLSPNAPS
                     SHNNLAAVALAERKTDEAMQHYERALSLDAASFDALNGIISLYMAQGKFDQAQARVDQ
                     AITARPDSASLYFLKAGIYGRLGGIAEGQGNAEQVKQYAQQAEEQLRLALKYDPTFVP
                     ALNSLGSLYVKTNRPDDAITEFRRMSEKRPDDPTPYMLIGMVEDGRKNYSASTEAYRR
                     ALNVSAESAFAANNLAWNYAEHGTGNLDEAVRLAQGVVQKFPEEPGFADTLGWVYYKK
                     GLHGAAVEQLQKAVRQTQARAAKGQGTDSALYRYHLGVALAALGRKPEARQQLQQALN
                     LNNGTKLSPENEQEARKALAAL"
     gene            complement(10903..11553)
                     /locus_tag="VGB76_10155"
     CDS             complement(10903..11553)
                     /locus_tag="VGB76_10155"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HEY0099300.1"
                     /translation="MKSKKWHFWALLAFLLIGGTVVNLWEHIGEASVVRRELKEFPTA
                     LGQWQQRGVDVRFDAQTESVLRADDYLSRDFGLPDGRYANFYVGYYETQRNGATYHSP
                     LNCLPGAGWTLNDPAAIQITPADGGQPFEANRYIIQNGDTRQLLIYWYQGRGRAVASE
                     YWGKIYTVVDSVKRRRSDGAMVRVIVPVHKSEADALALATDLASQAAPLLPGFVPN"
     gene            complement(11593..11892)
                     /locus_tag="VGB76_10160"
     CDS             complement(11593..11892)
                     /locus_tag="VGB76_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099301.1"
                     /translation="MTGEEMERAIEFLLQSQAKAEAQIAETARQIAETQQQAAETQRQ
                     AAAYAETQSVFIQTVTRALEAQDQINASLRAADARTDERLNKLIGLFEQHIKVGH"
     gene            complement(11933..12985)
                     /gene="xrt"
                     /locus_tag="VGB76_10165"
     CDS             complement(11933..12985)
                     /gene="xrt"
                     /locus_tag="VGB76_10165"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase"
                     /protein_id="HEY0099302.1"
                     /translation="MTALTRPQLWKPVIVAVAVLFVYATVLVKLGGQWWVDENYSHGL
                     LVPFIIGYILWSERERLAHAAGRPGLLWGGALVVLAVLMLWAGTAGAELFMQRTSLVV
                     MLAGITIYFWGWHILRLALVPLLLLALAIPIPAIIFNQIAFPLQLFASRCAVWAMRAF
                     DIPVLRQGNVIELMPLNSSVTKKLEVVEACSGIRSLMTLLTLAVVFAYFTHPRDPDKQ
                     GGGIVAALKRYGTWRSAILVLSAVPIAIITNAGRVSGTGILARYYGTEVADGFFHEFS
                     GWVVYIAAFLLLFALGWVLDKFGARRKPPQNKPPRKTAEATDATVNDESTETPVVASA
                     PTPATISLRERGADGS"
     gene            complement(13215..16232)
                     /locus_tag="VGB76_10170"
     CDS             complement(13215..16232)
                     /locus_tag="VGB76_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099303.1"
                     /translation="MNSPRLFAQRPFRLFSFRLNLVLALALVLLASSGGATFAQSGGI
                     KTDLGVYAPVGLPQLPAAGGKFRDPVFGTEIMRVTDSRDGADAGTSYSYWPTFNSDNT
                     RVLVMGAGGDAGCAIYDFDPVTFQLGAKQTLPLINGAYPFAFDLIWSHTEPDILYGHT
                     GAKLWRYNVRERSYSLAFDLTPRLPAGYYFSQASLSSDGDVFACTLQQSGTWAVVGYM
                     AYRRSTDTILYRSNDDLNEVRIDKSGRYLFVNTNDQGVGKVEVRIIDLATGQVTDLTD
                     DAPDHAPSHYDVGTGMAVGNGNYLAGISARPLSAPRSFSKILDLSTIKNYGGFHLSML
                     ADNEEWSLVSFYTPHQNLVMQGEVVQVATDGSGRVRRLFHHQSVLQTYYDSPRANISR
                     DGRFVAFSSNWGVTGGRHDMFVARIDPAPYSSASPTPTPTPTPNNEPRPSVPGDVVWF
                     EGAVPAGATVEADADMWDWVTHSPVSFSGRPAHRSSLHPAFHQHHFFNAFDTMKVSAG
                     DKIFTYVYVDPNNLPNELMLQWHVQGQGVEGWEHRAYWGASNINWGVEGTNSRRRMGG
                     IPAAGQWVRLEVSASEVGLEGKTVDGMAFTLHGGRASWDRTGRKTLVAATPTPTPTPT
                     PTNSLSAPALVAAARTTATTLASAPSVSAAEINALENNIEAAYAAFVREASQFHASGQ
                     IDASLRVSLYFARAAEALAAAGASSSSVQNRLQITAARLAQAYNAMLPPASSTTAAEN
                     AHAPAAATNLPFIGAANTFSSASMSPLLSPRSLGTITGDAAQSPLARAGVVADTSGNK
                     TLPLELEGASVSVGGHAAPLLYVSPARIAFVVPQGFSQEGEVEVIVTSQDGYVSRGTT
                     VIKAVAPALFTADASGAGSALVMNGSEPAASSFEVLSPHAFGADKRTRVMLYATGISA
                     GAANLYASNDVRTANGGTIANVGESLTVEARTADGRLYHLPVEFAGAVEGRLPGLDQV
                     NVLLHPELRGAGSVDLTIIINGQRSNTAAINIR"
     regulatory      complement(16245..16339)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      complement(16702..16822)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         3124 a         5182 c         5476 g         3456 t
ORIGIN      
        1 cgggcgcgcg tgatctcgga cgcaggagaa gacgcgtgct tcttctcctg tgcgcctctc
       61 gttttcactt tcgcatcttt tcgcgtctct tcctgcacag ccggagttgt tgccggaggg
      121 gcaactttgg cttgaggctt cgcggcgtcc tgcgcgaaga gggcggcggc agacgcggga
      181 gtcgcggcgc acgtcgcact gacgccgaag cagagcgatg ctgagagcac gaggcggcgc
      241 gcgaagcgtg cggagcgcgt gcgtgtgaaa ttcgagttcg acaattgttc cggcatctct
      301 atttctccgt cggatgtgag cgaaagtttc gggttcggga tgtgcgctac aagcgcgccg
      361 tgcggccttc ttggaagcgg cagcgattca atgctctcga cgcgtcgcgg aatttacgtt
      421 gacgcggcgt ggacgctctt tcggttgtcc cttcggttgt tcgttgccgg ggagtttcaa
      481 ctgcggcggc ggtgtagcct gcgcgtcgtc tgcgtatcga acaatcgtgc ccccgaagcc
      541 gaccgcccat gcgcgcgcgc ggctcgcaat cgagaggcgt tcgagcgggt ggcgcgtgcc
      601 gctcggttcg atttgccagt gcgcgccgcc gtccgtcgtg tgaatgatcg tgccctctgc
      661 gccgaccgcc caacccgtcc gtgcgtccag aaatttcacg tccgagagct cgacgcgcac
      721 gttcgattcc tgtgggaacc atgcgcgccc gccgttcgtg gtcacgagca tgaggccgcg
      781 ttcgccgacc gcccagccgc gccgctcatc tacgaaagcg accgcgttga agcgcgtcgc
      841 ggtgctgatg ggcgaactgc ccgcgcgcca cccttcaccg tcggggttgt acaggatggt
      901 gttgcccgcg ccgacgagcc agccctgacc ctcgttgaga aacgcgccgc cgagcagcag
      961 gtgtttcgtc ggcacaagct gccgcgccca cgtcgcgccg ccgtccgtcg tgttgtagag
     1021 cgcgcccatc tcgccgaaag cccagccgcg catctcttca acaaacgcga cgcgcgttaa
     1081 gagcacgtca acgtccgcgc ccgtcacttc gacgcgcgcc caactctcgc cgccgtccga
     1141 agtcttcatc aagtatgaac gcggttctgc tttcgtcttg agcaggtaga tgctgcggtc
     1201 gcagacgagc cagcccgtct cttcgtttgt gaaataaacg tcgcgcaggg tgtcttccgt
     1261 cgggcgacgg cgcacctccc aactcgcgcc cccgtccgtc gtcctgagca gcgcgccttt
     1321 actgccgacc gcccagcccg tccgttcgtc aacaaaatag accgcacgca accaggccag
     1381 cgtcccagac gtttgcgact cccacgcgcc cgccgccgac gcgattgcag gcgcgacgag
     1441 cagcgagagc aagacaacga aacgatgaag gataaacgat gagcgatgga cggaagaggt
     1501 gggatgcggg acatagaacg tcgaacgcgc agcacgcaac gtcgagcgaa gcgcaactct
     1561 cttcgtccgg gcgttccgcg ttccgcgttc atcgttcatc gtttcttaaa agccgccggt
     1621 gaagatgcgg aagaaactga cgacgggcag cagtttcatc acgcggtcga aggtcttcag
     1681 tttgttgccg ggcacgatca cttgatcgcc ggggttgaga aagtagttgt cgggcatgcg
     1741 tcccttctca atcgcggcga catcgatgat cgtcggttgc aggaagccgt tggcctgcct
     1801 tcgcatgacc atcacttttt tcttgttgcc cgtgtcgagc acgccgcccg acagagccag
     1861 agcctccgtc accgagaggc ggcgcgacat cgggcggatg cccggctgcg ccacgtcgcc
     1921 caccacccag aagaccgccg actgcgcctt gtcgagcgag acggtcacct tcggatcgat
     1981 gacatactcg tcgagccgct tcgtgatctc ttcggcgact tgcttcgtcg tcttgccgcc
     2041 tacgaagacg ccttcgggga tgagcgggtg cgagatgacg gcgtcgggcg gcacgatgat
     2101 gcctgctttc gagtagcgtt cgagattgaa gaccgtgatc gagatcacgt cttcgggacc
     2161 gagccggtag gtcgccatga agttgttgta gtaaggcacg acggcggctt cctcttcgga
     2221 gagcgactcg cggcggttcg ccagcatctc cgcagacatg tcttcgcgcg gcggcgcgct
     2281 caggtcgtca ggctccagac gctcgcccgc cttgcccttc gacgtgccgg aagcgttggt
     2341 cgcggtgcgc gggcgcgtct tctgctcaga ctttttctta tcttcctgca tcgtgtcgtc
     2401 ttcgccgaag acgcggaagt attgaactgt ctgcgagaga gaaggcttgc gcgcggcggg
     2461 cgcggacggc cttgctcctt cctgcgcttc ctgcgctgcg agcgcgccgg aaccgcctgt
     2521 gagcaggaag aacgtgaagg ctgccgttgc caacttcatc gtgtgaactt tcatttcatg
     2581 tacctcaaac taacttccaa tatcgcacgt gcgcttgggt tcagcgtgcg ccgcgaccga
     2641 aaaggatggt cttgaccgtc tcgaataaga tcaccgcgtc gagcactagg ctctggttct
     2701 tgatgtagta gagatcgtat tggagcttct gccgcgcgtc ttcgatggac gagccgtacg
     2761 gatacttgat ctgcgcccag cccgtcagcc ccggcgggat caaatggcgt tgttcgtaga
     2821 acggaatctc ctccgcgagt tgcgcgacga aatgcgggcg ttcggggcgg gggccgacga
     2881 agttcatctc gcctttgagg atgttccaga actgcggaat ctcgtccacg cgaatcttgc
     2941 gaatcacgcg ccccacgcgc gtcgtgcggt cgtcgcccgt ctgcgcccac acaggcccgt
     3001 tgcgctccgc gtccacgcgc atcgagcgaa acttcatgat cgtgaacggg cgtccgttct
     3061 ggccgacgcg ttcctgcctg tagagtgccg gacccggcga ttcgagcttg atcagcactg
     3121 cggtcaggag cgcgatgggc agcgacacga tggcaccgac gaaggcgacg ccgcgatgca
     3181 ggaacgcacg cgccacgccg ctcaggcgcg cctgcctgcc gcgccccgaa aagatcagcc
     3241 aagaggggcg catcatgttg agatgcacgc gtcccgtcgt gcgttcgtaa aacgatgcgc
     3301 cctcttcgat ggcgacgtgg ccgccgagcg agaggtcgag cagttggcgg gtgggaaatt
     3361 gcccgcgccg ctcgccctgc gcgacgatga tgcggtctat gttttcgcgc ttgacgatct
     3421 gcgccatgtc ggtcgtgacg ccgatgacgc gcgggttgat gatgctctta ccgacgagcg
     3481 cggggtcgtt gtcaacgaaa ccgacgatgc gataaccggc gtcgcgtcgt tccagaacct
     3541 cgcgcgcgat ctcgattgcc gccgtgcccg aaccgacgat caaaatgcgt tcgccgacca
     3601 tcggatggcc gagcagccag tggatcgaga tgcgccacgc gaccatcaag gcgagcgcga
     3661 gcgggagcgc gatgagcgag atgccgcgac cgagcatgag ttgcggcaac aggtaaaaga
     3721 ctatcgccag cgcgatccag gcgaggccga gagcctgtat gagcctgagg acgagttcgc
     3781 gccggtcgtg catgacgacg aaatcgtaga ggtcgtagag atagaacgcg ccgaggcaga
     3841 aggccgtggc gagtgcggcc ttgtaaaaac cgttgcgccc gacgagttcg taataagcgt
     3901 cgtgcgagcc gaggcgaatg tgcgcagccg ccaccatccc gccgaacatc aacagggatt
     3961 cggccagaat cagcagcggc gtgcgcgcat tgaaccgacg cgtcggactc aagagatctc
     4021 cttcggaagc gacgagtggc gagtgacgag tgaaccgtga atagagaata gagaaaaact
     4081 gtcttcttct atgatagaga aaaacggtct tcttctattt acgtttgacg atttacgatt
     4141 cacgtttttt ctctcgtccc ttgtcacttg tcactcatca ctgctctttt gtcactgttt
     4201 ctttgcggta gtagcggcgt tggtagtagc tgctgctgtc gtctttgttg tcgtccgcgc
     4261 cgttgaggat gatcccgagg aggcgttcgc gcgggagcgg ttcgagcacg cggtcgagcg
     4321 cggcgtagcg tgtcttgccg gaacgcacga ccatcagcgc gccgtcggcg cgaccgatga
     4381 gcacggtggc atccgtgaag atgccgagcg gcggcgcgtc gatgatgatg tagtcgaaca
     4441 tgcggcgcag ttcgccgagg agcgtgctga acttcggacc cgacagaatc tccgcgacgt
     4501 tatcgcgcac cgtgccgccc ggcaacagat gcagtccggc gggttcgagg cggatgatgg
     4561 tatcggccag agtcgcctcg cccgcgagca cttcggagag tccggcgggc gcgtcgatgc
     4621 cgaggtaatc ggcggcgcac gggttgcgga ggtcgccgtc gatcaggagc gcgcgcacgc
     4681 cgtcggtctg cgcgagcagc cacgcgagat tgagggcggt gacggtcttg ccttccatca
     4741 cgcccgcgct agagacgacg atggcctgca cacgccgacg ctcacccgca tgaaggatgc
     4801 gcgtgcgcag ggcgcgatac tgttcggcgt gcggcgagcg cgggtcggtg atggcgatga
     4861 ggtgtggttc gacgcgcgcg tgagaaatct cctgcgaagg aaactccagc gcgcgcgccg
     4921 aactgacagc gtccgccgtt gccccagcaa cacgcgaagc ctgcccgccg gggagtgccg
     4981 acccggctgt ggctgcatcg atgtgctcag cggacgctga aacgtgcgcc ctgttttcag
     5041 gcgcgttgaa gagcgcggac gtgcgccgca ggacgacctc cggcaccgca gcggaagggg
     5101 cgtggttgcc gccgtgatgg tcgccgttcg agttgccgtt tgccggcgcg gacgcttcgg
     5161 ttaagagcgt tacctcttcg tcttcgacaa ccgcagccgc agtcgaagcg cggcgtgctg
     5221 tcgtctcgtc cttgctcttc tgatctgcgc tgccgttctc ggcggcgcgc ttcaatgctt
     5281 cataaactct gcccatcacc tatctcccat cacatcccaa cgacggaagg ccgcgccgat
     5341 gtcgttcaca tcgggcgaag gccgcgccgc cgtcgaagct ctctcttcca actcttcatc
     5401 ctctgcgcgc gtgtgcgccg aagaatttgt gtctgacgtt gcaatcactt cgtcaaccgc
     5461 actcgcctgc gcgctgcccg cagaccacaa ctcggcgcgt ccctgcgcgt tgaaaatgcg
     5521 cgcgggcaga tcacgctcct cgtccggcag gcgactactg ccgccgcccc ggcgcgaatc
     5581 gggcagcatg tcgaacgtat cggccacctc ttcgatcacg gcgcgcggga tcgtgtgctg
     5641 cccggcggca tagcccgtca gcagagcgtt gtcgcagagg ttattgatct ggcgcgggat
     5701 gcctgcggcg cagcgaaaga tgtaatctat cgcgccgggc gagaagatgt ccgtgcgcgc
     5761 cgcgcccgcc acgagcaggc gcgctttgac gtaacgctcc gtctcctcga tgttcggcag
     5821 aggcttgatc tcgcagcgca gtgcgacgcg ctgtttcaac tggcggaggt ctgggttgtt
     5881 caagacctcg cgcagttccg gttgcccggt caaaacgatc tgcaactgct tggcggagtc
     5941 ggattcaaaa ttcgagagca gcctgatctc ttcgagcacg gcggttgaga ggccgtgcgc
     6001 ttcgtctatg atgagcacgg tgcgcaggcc gcgcgcgtgg cgggcttcta aagtttggcc
     6061 gagggcgaag aggagttccg acttgttctc ccattcctgc acgtcgaaga ggcgcgcgac
     6121 gtgttgataa aattcgggga cggtgagacg cgggttgaag atgtagccga cgagcacggt
     6181 gcggtcgagc cgctgcatca tccagcgcag gatcgtggtc ttgcccgtgc cgacttcgcc
     6241 cgtcacgacg atcaaaccct tgccgctctc gatgccgtaa tgcagattcg ccatcgcctc
     6301 cgtgtgcgaa ggcgtgaaat agatgaagcg cgggtcgggc gtcagcgcga acggcaactc
     6361 ttttagtccg tagaactctt tgtacaactc tcaacctctt ttcaaagtga tgagtgatgg
     6421 gtgatgagtg atgagtgaaa gacagctctt tggtttcgac tcattactca tcactcatca
     6481 ctgtcacgag acgaacagag tgaagacgtt cgtgaagcgc agcaagagcg cgagcgcggg
     6541 gatgctcaag acggtgagcg taaggctggc aaaggccgtc gccatgcgtc gcaggcgtgc
     6601 gcgccgctgc tccttcggcg tcaggagttc gggcacggag atgagcacgg gcaagcccgt
     6661 gtaatggcgt gcgtcttcga cggtctgaat cgtcagcagg cgcggcactt caaaggcggc
     6721 ggcgaaggcg aggccgaagc cgaggccgag cgcgaggccg agcgcgatga gcataggacg
     6781 ctgcggcgcg atgggacgca cgggcaaact cgcagggtcg atcacctgaa tcgtttcgcc
     6841 ctgcgccgag ttggtcgcgg cgacgttcag gtcggacttc cctttcttca acagcaactc
     6901 gtcataggcc gctttcttcg tctggtactc gcggtctaga gagccgagcg cgacttcggc
     6961 ctgcggcacg cggctcaact ggctctgcac agaggcgatc tgcgcgccgg tttgcgcgag
     7021 caacttctcc tgccgcgcaa cctcgccctc gatggcttgc aggttggcct tgatcgtgta
     7081 gctgcgcgga tcgacgcgcc gctccacgcg cttcgtcttc tccgcgatgc ggttcttcca
     7141 atcgtccgtc aactcctgct tgctgcgctt aacggcgtcg agttcctgac gcttggcgac
     7201 cacgtccggg ttcttcggct tcaagaccgt gagcaggttt tgcaactcgg aatcgagttg
     7261 cgattcgcgc cgcgagagtt cagcccaggc gggcgtcgtc ttcgggtcgg tcaaagtctc
     7321 gaccacgtct tccttctcga tctggtattg cttctcgatg tcgccgagcg tcgtgttgag
     7381 cagcgtcttt tgatcgcgga ggcgtccgat ctcgttgatg aaagccttct gctgttcgta
     7441 gaggccgctc agattcgtga cgagcgaagt ggcctgcgtc gggaggttgt tgatgttcga
     7501 ttgcagggtt tcgaggcggc gtttgtcaac ggcgtcaacc tcggccttcg ccacgtcgag
     7561 ttgctcctgt aggaacttcg cggtttccac cgcgccctgc gattgctgtt tgatctgcgc
     7621 gttgacgtac ttgctcgcga gatcgcccgt caccttctgc gtgatgtagg ggtcggctcc
     7681 tttgaaggtg agaatgaaac cgttggtgat gtcgttgcgg ctcttgttga cttcgatccg
     7741 cgtgtccttc ttcgccatgc gttcgacgag catttccatc ggctcaccgc gcaggcgttc
     7801 ggcggcgtag aggttgtaag tggtgatcag cggttcgagc gacgaacggc tgaagacctc
     7861 ctgcccgatg ttgttgatgc ggatcgtgag atcgtcgtcg gagagttgcg gcacgatgcc
     7921 ctgcatgatg ttcgaggggc gcactgtcag cagcgtgctc gattgataga cgttcggcag
     7981 acgccacacg acgatggcga ccgccgccga gatgacgatg gccggaagga tgatcagcca
     8041 cttgcgtttc cacaaaatct gcccgtactc gcccggcttt ctttgtcgaa attcaacact
     8101 cataatttag acctcactcc aaaaactcga cctctcgcgt tcgcgcccga aatcacagag
     8161 cggcgagcgc cttgcgcgct tcctgttcat tctccggcga gagtttggtg ccgttgttca
     8221 ggttcagggc ttgctgcaat tgctggcgcg cttcgggctt gcgtccgagg gcggcgagcg
     8281 caacgccgag atgatagcga tagagcgcgc tgtctgtgcc ctgtcccttg gcggcgcgcg
     8341 cctgcgtctg ccgcacggcc ttttgcaact gctcgacggc ggcgccgtgc aagcctttct
     8401 tgtagtagac ccagccgagc gtgtctgcga agccgggttc ttccgggaac ttctgcacga
     8461 cgccctgcgc gaggcggacg gcttcgtcga ggttgcccgt gccatgctcg gcgtagttcc
     8521 aggcgaggtt gttggcggcg aaagccgatt ccgcgctgac gttcagagcg cgcctgtagg
     8581 cttcggtcga ggcgctgtaa tttttgcgcc cgtcttcgac catgccgatg agcatgtagg
     8641 gcgtcgggtc gtcaggccgc ttctcgctca tgcggcgaaa ctccgtgatg gcatcgtccg
     8701 gacggttcgt tttgacgtag agcgagccga gcgagttgag cgcgggaacg aaggtcggat
     8761 cgtatttgag cgcgagacgc aactgctctt cggcctgctg cgcgtactgc ttcacctgct
     8821 ccgcgttgcc ctgcccctcg gctattccgc cgagacgccc gtagatgccc gctttcagaa
     8881 aatagagcga cgcgctatcc ggcctcgccg tgatggcttg gtctacgcgc gcctgcgcct
     8941 gatcgaattt cccctgcgcc atgtagagac tgatgatgcc gttgagcgcg tcgaaactgg
     9001 ccgcgtcgag cgagagcgcg cgttcgtagt gctgcatggc ctcgtcggtc ttgcgttcgg
     9061 cgagcgcgac ggcggcgaga ttgttatgcg aagagggcgc gttcggcgag aggtcgcgcg
     9121 cggcgataaa atcggcgcgg gcggcagacg tgttcgaagg ctgcgactgc aagaaagagg
     9181 tggcgcgcgc ggtcagcgtt ttggcgcgca gttcgtttaa gagttgcggc gagttgcgcg
     9241 cgtcgggcgc ggctttgtcc agacgcgcga gcagttcgtt ggcgaggcgt ccggcgttcg
     9301 cagcatctcc ggcggcgagg ttgatctgca cctgcatcaa cttgcccgga agataatcgg
     9361 gataaaactt ttcgaggtcg cccgcgaaga tgcgcgactg ttccatctgc ccggcgcgga
     9421 aacaggcttc ggccatgaag tagagtccgg cttggtcgcg cggctcgatc ttcaagacct
     9481 gcttgaggtc ttcgatggcg tctttcggac ggtcttgatt gagacgcagg cgtgcgcgca
     9541 ggatcagagc ttcgcggtcg ctggcattgc gcttgatggc ctcttcgacc tgcgccgccg
     9601 cgccctgctg gtcgccgcgt tgcagcatca actcggcgag ccggtgacga ccgcgcgcat
     9661 aatctgccga cttcgccacg atgtcctgat agatgccagc ggcttcgtcg taacgaccga
     9721 cggaggcgta gaaatccgcg agcacggcgc gtccttcggg tcggtcgcgg tcgagctcgg
     9781 cgagagcttt gaacgactct tcggcgcggt cgagttgttt gttgatgaga tagaaactgg
     9841 cgagcgtgcg acgcgcatcg cggttcgtcg gttcaacttc gacggcgcgc ttgaactcga
     9901 cttcggccat gtcctgccgg ttctgcgaaa cgaagaactt ggcgtattcg agatgcgcga
     9961 gcgcggagcg gtcgttgatg gagagcgcgc gtttataaac ttcttcggcc ttcgccgggt
    10021 cgttcatctg acgatggaag agcgcgaggc tcaagagcga ctccacgcgt tgcgggttga
    10081 gttcgacggc gcgattgagc gcggcgagcg cctcctcgcg cttgccggag gcgaagagca
    10141 cggtgccccg caggatgtgg ccttcgatgt aattcgggtc tttgtctaag acctctttgg
    10201 cgatccgctc ggcttcggcg cggagtttgt cgtccttcgt ctgctggaaa gcgaggatgt
    10261 aatagttgcc gagacgcacg cgcgcttcca gattgttcgg gtcgagttcg acggcgcggc
    10321 gcaactcttc gaaggcctgc gtgaactgct ccatgccttc gtaggcgcgt gcgagtcccc
    10381 agtgcgcctg tgcgaggcgg tcgtcaatct gcacggcgtt gcgaaattcg agcgaggctt
    10441 cctgaaactt cttctcggtc aggtaggctt cgccgcgctg gacgtgttcg gctttggctt
    10501 tttcggggtt ggagcaggca agggtcgcaa acgcgaaggc ggcgacgacg aaggcgagca
    10561 gaacagacga cagactccgg ccattgccgg agcgcgggca ctctttgtaa ttcatctcgg
    10621 tcttttagac tccttcgcgg ctctcgctaa gctctgtaac cggggcaacg gtcgtttgag
    10681 cagcagccgc gtctctgttg ttaagtgaag ccgtctttgg ttaacggttc ttttaagccg
    10741 tctgcgagtg gggcacaccc cgaacggcgt aaaacttcac actaataaaa ctggcaatcg
    10801 tttttttctt cctgaaatcc ggcggcttaa atcttcccca agagccgccg cgcatttatg
    10861 ttaccacttc ccacacgttc agcgatcaac tttgttgcta ctttaattcg ggacgaaacc
    10921 cgggagcagc ggcgcggcct gcgacgccag atcggtagcc agcgcgagcg cgtccgcttc
    10981 cgacttgtga acgggcacaa tcacgcgcac catcgcgccg tccgaacgcc tgcgcttgac
    11041 gctatcaacc accgtgtaaa tcttccccca gtattcgctc gccacggcac gcccgcgccc
    11101 ctgataccag tagatgagca actggcgcgt gtcgccgttc tggatgatgt agcggttggc
    11161 ctcgaagggc tggccgccat ctgcgggcgt gatctggata gcagccggat cgttcagcgt
    11221 ccagcccgcg ccgggcaggc agttgagcgg gctgtgatag gtcgcgccgt tgcgctgcgt
    11281 ctcgtaatag ccgacgtaga aattggcgta gcgtccgtcg ggcagaccga aatcacgcga
    11341 cagataatcg tcggcgcgca gcacgctttc ggtctgcgcg tcgaagcgca cgtccacgcc
    11401 gcgctgctgc cattgtccga gagccgtcgg aaactctttc aactcacggc gcaccacgct
    11461 cgcctcgccg atgtgctccc acaaattaac gaccgtcccg ccgataagca gaaaagcgag
    11521 caacgcccag aaatgccact tcttcgattt catatcttca ctcttagcag aacgcggcag
    11581 gaaaccgccc ctctagtgtc ccactttgat gtgctgttca aacagcccga tcaacttatt
    11641 caaacgctcg tccgtacgcg cgtcggcggc acgcagcgag gcgttaatct ggtcttgcgc
    11701 ttcgagcgcg cgtgtcacgg tctgaatgaa aacgctctgc gtctccgcat aggccgccgc
    11761 ctgtcgctgc gtctccgccg cctgctgctg cgtctccgct atctgtcgtg ccgtctccgc
    11821 aatttgcgct tcggcctttg cctgactctg caacaggaat tcaatggcgc gttccatttc
    11881 ttcgccggtc atggattgct cccttcctca tcgtgcaggc taacagcttc tttcacgatc
    11941 catccgcgcc gcgttcgcgc aaagagatcg tcgccggagt cggcgcagac gcaacgacgg
    12001 gtgtttcggt tgactcgtca tttaccgtcg cgtcggtcgc ctcggcagtt tttcgtggag
    12061 gcttattttg gggaggcttc cggcgcgcgc cgaatttatc gagcacccag ccaagcgcga
    12121 agagcaggag gaaagccgcg atatagacaa cccagccgga gaactcgtgg aagaaaccgt
    12181 cggccacttc cgtcccgtag taacgcgcga ggatgcctgt gccgctcacg cgcccggcgt
    12241 tggtgatgat cgcgatgggc actgcggaga gcacgaggat ggccgaacgc cacgtcccgt
    12301 aacgcttcaa agccgcgacg atgccgccgc cctgtttgtc cgggtcgcgc gggtgggtga
    12361 agtaagcgaa gacgaccgcg agcgtcaaca gcgtcatcaa cgaacgaatg ccgctacagg
    12421 cttcgaccac ttccagtttc ttcgtgacgc tcgaattcaa aggcatcaac tcgatgacgt
    12481 tcccctgccg caaaaccgga atgtcgaacg cgcgcatcgc ccacacggcg cagcgcgacg
    12541 cgaacaactg caagggaaac gcgatctggt tgaagatgat ggcggggatc gggatcgcga
    12601 gtgcgagcaa cagaagcggc acgagcgcga ggcgcaggat gtgccagccc cagaaataga
    12661 tcgtgatccc ggcgagcatc acgacgagcg aagtgcgctg catgaaaagt tccgcgcccg
    12721 ccgtgcctgc ccagagcatc agcaccgcga ggacgaccag cgcgccgccc cataaaagac
    12781 ccggacgccc ggcggcatgc gccagacgct cgcgctcgct ccacaggatg tagccgatga
    12841 tgaacggaac cagcagaccg tgcgagtagt tttcgtccac ccaccattgc ccaccgagct
    12901 tgacgagcac tgtcgcatag acgaagagca cagcgacggc gactattacc ggcttccaga
    12961 gttgggggcg tgttaaagct gtcattcttt gcttttaaac ttcaggaaaa ctttatgcac
    13021 ggaagaggca ggccagcgca cgagcactga gaacaaccac tcgcgctgtt taagagagta
    13081 acaaaagggg agcacggggg gcaacagcaa aaggcgcgag ggaaaagggc gaggggtcga
    13141 ggggaaaggt aaaaagcagg aaactctctc ttcttttctt ccctttcccc ttgcctcctc
    13201 gcccttttcc cgccctatct gatgttgatc gcggcggtgt tgctgcgttg gccgttgatg
    13261 atgatcgtga gatcgaccga gcctgcgccc cgcagttcgg gatgcagtag gacgttgact
    13321 tgatcgaggc cgggcaggcg accttcgacc gcgcccgcga attcgacggg cagatggtag
    13381 agccgcccgt cagcggtgcg cgcttcgacc gtcagcgatt cgccgacgtt cgcaatcgtg
    13441 ccgccgttcg ccgtcctcac gtcgttgctg gcgtagaggt tggccgcgcc cgcgctgatg
    13501 ccggtcgcat agagcatgac gcgtgtgcgt ttgtctgcgc cgaacgcgtg cggcgaaagc
    13561 acttcgaagg aagaggccgc cggttcgctg ccgttcatga cgagcgcgga gcctgcgccg
    13621 ctcgcgtcgg cggtgaagag cgcgggcgcg acggctttga tgaccgtcgt gccgcgcgag
    13681 acgtagccgt cctgcgaggt cacgatcact tccacttcgc cttcctgcga gaatccctgc
    13741 ggcactacga aggcgatgcg cgcaggcgag acgtagagga gcggcgcggc gtgtccgccc
    13801 acagagacgc tcgcgccctc cagttctaag gggagagtct tgttgccgct cgtgtctgcg
    13861 acgacgcccg cacgagccag cggactctgc gccgcgtcac ccgtgatcgt gcccagactg
    13921 cgcggcgaga gcaggggcga catgctcgcg ctcgaaaagg tgttggccgc gccgatgaac
    13981 ggaagattgg tggccgctgc gggagcgtgc gcgttctctg cggcggtagt gctgcttgcg
    14041 ggcgggagca tcgcgttgta cgcctgcgcg agacgcgccg cagtgatctg caagcggttc
    14101 tgcacgctcg acgaactcgc gcctgccgcc gccagagctt ccgcagcgcg cgcaaagtag
    14161 agcgagacgc gcaggctggc gtcgatctgc ccgctcgcgt gaaattgaga ggcttctctg
    14221 acgaaggccg cgtaagccgc ctcgatatta ttctccagcg cgttgatctc ggcagccgag
    14281 acggagggcg cagacgcaag cgtggtcgcc gtcgtgcgag ccgccgcgac gagggcgggc
    14341 gcgctgagtg agttcgtggg agtcggcgtc ggagtcgggg tcggcgtggc ggccacgaga
    14401 gttttgcgtc cggtgcgatc ccaactcgcg cgcccgccgt gcagcgtgaa ggccatgccg
    14461 tcaacggtct tgccttcgag gccaacttca ctcgcgctca cttcgagccg cacccactgt
    14521 ccggcggcgg gaatcccgcc catccggcgc cggctgttcg tcccttcgac tccccagttg
    14581 atgttcgacg cgccccagta agcgcggtgc tcccagccct ccacgccctg cccctgcaca
    14641 tgccactgca acatcaactc gttcggcaga ttgttcggat caacatagac gtaagtaaaa
    14701 atcttgtctc cggcggagac tttcatcgtg tcgaaggcgt tgaagaagtg atgctggtgg
    14761 aaggccgggt gaaggctgga cctgtgcgcc gggcgaccgc tgaacgacac ggggctgtgc
    14821 gtcacccagt cccacatgtc cgcatcggcc tcaaccgtcg ctcctgccgg aactgcgccc
    14881 tcgaaccaaa ccacgtcgcc cggaacagaa ggcctcggct cgttgttggg cgtcggggtc
    14941 ggcgtcggag tcgggctggc ggatgaatag ggagcggggt cgatgcgcgc gacgaacatg
    15001 tcgtgccgac ctccggtcac gccccaattg gaactgaagg cgacgaagcg accgtcgcgc
    15061 gagatgttgg cgcgcgggct gtcataataa gtttgcagga ctgactggtg atgaaagagc
    15121 cgtctcaccc tgcccgaccc gtccgtcgcc acctgcacca cttcgccctg catgactaga
    15181 ttctgatgcg gcgtgtagaa gctgacgagc gaccactctt cgttatccgc cagcatcgag
    15241 agatgaaagc cgccgtaatt tttgatcgtc gagaggtcga ggattttcga gaaggagcgc
    15301 ggcgcggaga gcgggcgcgc ggagatgccc gcgagatagt tgccgttgcc gacggccatg
    15361 cccgtgccca cgtcataatg cgaaggcgcg tggtccggcg cgtcgtccgt caagtccgtc
    15421 acctgtcccg tcgccagatc gatgattcgc acctcgacct tgcccacgcc ctgatcgttc
    15481 gtgttcacga agaggtaacg cccggacttg tcgatacgca cttcgttgag atcgtcgttc
    15541 gagcgataga ggatggtgtc ggtcgaacgc ctgtaagcca tgtaaccgac gaccgcccag
    15601 gtgcccgatt gctgcaaggt gcaggcgaac acgtcgccgt cgctgctcag gctcgcttgc
    15661 gagaagtagt acccggcggg caggcgcggg gtgagatcga aggcgagcga atagctgcgc
    15721 tcgcgcacgt tgtaacgcca gagtttcgcg ccagtgtgac cgtagagaat gtccggctcc
    15781 gtgtggctcc agatgagatc gaaggcgaaa ggatacgcgc cgttgatcag agggagcgtc
    15841 tgcttggctc ccaactggaa ggtgaccgga tcgaagtcgt aaatagcgca tcccgcatcg
    15901 ccgcccgcgc ccatcacgag cacgcgcgtg ttgtcggagt tgaaggtcgg ccagtaggaa
    15961 taggaagtgc ccgcgtctgc gccgtcgcgc gagtcggtca cgcgcatgat ctccgtgccg
    16021 aagaccggat cacgaaactt gcccccggcg gcgggaagct gtggcaaccc gaccggcgcg
    16081 tacacgccca agtccgtttt aattccgccg ctttgcgcaa acgtcgcccc gccgctcgat
    16141 gcaagcagca cgagcgcgag agcgagaact aagttgagtc tgaaagaaaa gagacggaag
    16201 ggtcgttgag cgaacaaccg ggggctattc atctttggcc tcacttcggt aaccggacca
    16261 tctcacggcc ttcgttgcga acgccttgag actcgcggct ttgcggaccc gcatcactac
    16321 gggggtgcct ttaaatcggt gattggtgtg tgaaggaaga gggcaggcag cgcggcggaa
    16381 gcaacagcgg agacacggct aaccgggaac agtcgaagcc gttctcctca agcaactgca
    16441 acgtgcctgc ttgcacttga agagtaaaag catgtgttga cgtgagcggg ttctaagcag
    16501 cggaaggtta gacgggaatg aagagtggga attataacga caacaaaggc tgcacgcgga
    16561 taatcccgcc gtgaattcga actgcgaggc gagaacgttg acgcttccgg tgtcggagag
    16621 ctttgaagga aagggttctc gctcgaactc gtgagcaact cgcccaacgg agattcgaag
    16681 ccacgacttt cttcgatcgc cttcggcaac cagacaatct cccggacgtt gcgccccgca
    16741 tcaaaaatcg acgccggaaa cactatcgga gagactcacg gctttgcgcc ctcgcctcgc
    16801 gacaagtttg ccattatcgt gtgcaatctc tacctgaaag agcaatgctg cacgtcactc
    16861 cgttcggagg cagcagcgaa caacaaggat taaacaatat ttatactggt ttgtcaataa
    16921 atcacttttg ttaaatccgc cacggaaaat tcgccataaa aaccgcgcgt tcggggagtt
    16981 ttcgtagctg tattttctga atttataagg atttagcagt ttattcttga cgtgggttgc
    17041 gggttggtgc aatctgcctc gtggatttcc tgaaacactt gccttgagtg tccggttaat
    17101 aaacttcagc ttcacccaag agagcttgca cgtcatgcga ttgccctcca aactgctgcc
    17161 gggattcctg cttttcgcct ttttaattct agccaacgat gcgcgtgccg agcatatcgt
    17221 aatcacgagc ggcatcgc
//
LOCUS       DASWNS010000287         3342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_116062, whole genome shotgun sequence.
ACCESSION   DASWNS010000287 DASWNS010000000
VERSION     DASWNS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116062"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3261
                     /locus_tag="VGB76_10175"
     CDS             <1..3261
                     /locus_tag="VGB76_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0099304.1"
                     /translation="YAEAQRTLLTLLGNLPGMAYCCRNDEDWTMLYVSEGCLELTGFT
                     PADLTGNQKVSYASLIHPDDRQRVRVEVQSALAERTRFRFVYRLHNAAQGERWVWEQG
                     RGIFSPEGDLLAIEGFATDVTDRVQSEEALREAELKYRSIFEQAVHGIYRSTHDGHFL
                     AANPAMASMLGYATPAELINSCTDIAHDFYADADTRTRLQNLLAAHGKVQGFEARVFC
                     KDRSVIWMRENIYAVRDAAGALLYYEGSVEDMTARKRAEEMLARSEEQYRVLFESNPN
                     PMWVYDAGTYAYLAVNEAAVRHYGYTREEFLSMSILDVRPSDSIPAVIENITAKPSEH
                     RYAGEWEHRKKDGTRIFVEVSSQQIDFGGRAARLVLVNDVTERKHAVEALRESEERYR
                     ELFENANDVIYTTDLTGRYLSVNPVVEKVTGYTCAEICRMSFAQLLAPEYVELAHQMM
                     QAKLAGDETTTFYEAEIISKNGERVLLEVSSQLIYDEGVAVGVQGIARDSTKRKRAER
                     ALRESEERYRELFENANDLIYTHDLAGYFTSLNKTGERIIGYTREEAMQLNISQIVAP
                     EHLARAREMMTRKNFIEEATVYELEIIAKDGRRVPLEVSTRLVYVNGEPCGVQGIARD
                     IAERKHTEAKLLHNAFHDALTGLPNRNLFTDHLQMAVERARRHDEFVFAVLFLDLDRF
                     KNVNDSLGHTVGDGLLVALSRRLQHCLRPTDVVARLGGDEFAILLNDIGEQPGDAVLL
                     AERVQQELMQPFNLGGHEVFTTASIGIALSTTGYETPEAVLRDADTVMYRAKAQGKAR
                     HAVFDQNMHARVVALLRLENDLRRAVERNELRLHYQPIVALESGRISGFEALVRWQHP
                     ELGFIQPKDFIPVAEETGVIVELGQWVLSEAARQMRVWQAISPEHAGLKLSVNLSSRH
                     FVQPDLYERIVEVLDQTGLAPPMLQLEITESVLMENAQTIVPLLERLRELGVELAIDD
                     FGTGYSSLSYLSRLKPIHTLKIDRSFISMGGDDRENAEIVRTIVMLARNMGKDVVAEG
                     IETVEQLARLQTLDCTYGQGYLFSRPQDPVETERRLRAQGLNAPIDAPLTGELTAA"
BASE COUNT          702 a         1065 c          999 g          576 t
ORIGIN      
        1 tatgcggaag cacagcgtac gctgctcaca ctgctcggca acctgcccgg catggcctac
       61 tgctgccgca acgacgaaga ctggacgatg ctctacgtca gtgaaggctg tctggaactg
      121 accggcttca cgcccgccga cttaaccggc aatcaaaaag tctcttatgc cagcctcatc
      181 catccagacg accggcagcg tgtccgggtt gaggtgcagt ccgcgctcgc agaacggacg
      241 cgctttcgct tcgtctatcg tctgcacaac gcggcgcagg gtgagcgttg ggtctgggaa
      301 caggggcgcg gcatattctc gcccgaaggc gacctgctcg ccatcgaagg tttcgccacg
      361 gacgtaaccg accgcgtgca gtcggaagag gctctgcgcg aagcggagtt gaaatatcgc
      421 tcgatcttcg agcaggccgt ccacggcatc tatcgctcga cgcacgacgg ccattttctc
      481 gccgccaatc ccgcgatggc gagcatgctc ggctacgcca cgcccgccga gttgattaac
      541 agttgcaccg acatcgcaca cgatttttac gccgatgcag atacgcgcac gcgtctgcaa
      601 aatctgctcg ccgcgcacgg caaggtgcag ggcttcgagg cgcgcgtctt ttgcaaagac
      661 cgcagcgtga tctggatgcg cgagaatatc tatgccgtac gcgatgcggc gggcgcgttg
      721 ctttactacg agggcagcgt cgaagacatg accgcacgca agcgcgccga agagatgctc
      781 gcgcgctccg aggagcagta ccgcgtcctg tttgagagca acccgaaccc gatgtgggtc
      841 tacgacgcag gcacctacgc ttacctcgcc gtcaacgaag cggcggtcag gcactacggc
      901 tacacgcgcg aagagttcct ctcgatgagc attctggacg tgcgcccttc cgacagcatt
      961 ccggcggtga tcgaaaacat cacggcgaag ccgtccgagc atcgctacgc gggcgagtgg
     1021 gagcatcgca agaaggacgg cacgcgaatc ttcgtcgagg tcagttctca acagatcgac
     1081 ttcggcgggc gcgcggcgcg tctcgtgctc gtcaacgacg tgaccgagcg caagcatgcc
     1141 gtggaggctc tgcgcgaatc cgaggaacgc taccgcgagt tgtttgagaa cgccaacgac
     1201 gtgatctaca cgacggactt gaccgggcga tatctgtcgg tcaacccggt ggtcgagaag
     1261 gtgacgggct acacctgcgc cgagatttgc cggatgagct ttgcccaact gctcgcgccg
     1321 gaatatgtag aactcgcgca tcagatgatg caggcaaaac tcgccggaga cgaaaccacc
     1381 accttttatg aggccgagat catctcgaag aacggcgagc gtgtgctgtt ggaagtgagt
     1441 tcgcagttga tctacgacga gggcgtggcc gtcggcgtgc agggcattgc gcgcgacagc
     1501 acgaagcgca agcgcgccga acgcgccctg cgcgaatcgg aggaacgtta ccgcgaactc
     1561 ttcgagaacg ccaacgacct catctacacg cacgatctcg cgggctactt cacctcgctc
     1621 aacaagacgg gcgagcgcat catcggctac acgcgcgaag aggccatgca actgaacatc
     1681 agccagatcg tcgcacccga acatctcgcg cgcgccagag agatgatgac gcgcaaaaat
     1741 tttatcgagg aagcgaccgt ctacgagttg gagatcattg cgaaagacgg gcggcgcgtg
     1801 ccgctggaag taagcacgcg cctcgtttac gtgaacgggg agccgtgcgg cgtgcagggc
     1861 attgcccgcg acattgccga gcgcaagcac acggaggcga aactgctcca caacgccttt
     1921 cacgatgcgc tcaccggcct gccgaatcgc aacctcttca cagaccactt gcagatggcc
     1981 gtcgaacgcg ccagacgcca cgacgagttt gtcttcgccg tcctgttctt agacctcgac
     2041 cgtttcaaga acgtcaacga cagtctcggc cacacggtcg gcgacggatt gctcgtcgcg
     2101 ctctcgcgcc gcctgcaaca ctgcctgcgc ccgaccgacg tggtggcgcg tctcggcggc
     2161 gacgaattcg cgatcctctt aaacgacatc ggcgagcagc cgggcgacgc ggtgttgctc
     2221 gccgaacgcg tgcagcaaga gttgatgcag cctttcaatc tcggcgggca tgaagttttc
     2281 actacggcga gcatcggcat cgctttgagc acgacgggct acgagacgcc ggaagcggtc
     2341 ttgcgcgatg cggacacggt gatgtatcgc gccaaagcgc agggcaaggc gcgccatgcc
     2401 gtcttcgatc aaaacatgca cgcgcgcgtc gtggccctgc tgcgtctgga gaacgacctg
     2461 cgccgcgccg tcgagcgcaa cgagttgcgc ctccactatc aacccatcgt cgcgctggag
     2521 agcgggcgca tctccggctt cgaagcgctc gtgcgctggc agcacccgga actcggtttc
     2581 atccagccga aggacttcat ccccgtcgcc gaagagacgg gcgtgatcgt cgaactcggc
     2641 cagtgggtct tgagcgaggc cgcgcgccag atgcgcgtct ggcaagccat ctcgcccgag
     2701 cacgccggac tcaaactcag cgtcaatctc tccagccgcc atttcgtcca acccgatctc
     2761 tacgagcgca tcgtcgaggt gttagatcag acggggcttg ccccgccgat gctccaactc
     2821 gaaatcacgg agagcgtctt gatggagaac gcgcaaacca tcgtcccgct gctcgaacgc
     2881 ctgcgcgaac tcggcgtcga actggcgatt gacgacttcg gcacgggcta ctcgtcgctc
     2941 agttatctct cgcgtctcaa gccgattcac acgttgaaga tcgaccgctc gttcatcagc
     3001 atgggcggcg acgaccgcga gaacgcagag atcgtccgca ccatcgtcat gctcgcgcgc
     3061 aacatgggca aagacgtggt ggccgaaggc atcgagacgg tggagcaact cgcccgcctg
     3121 caaacgctcg actgcaccta cggtcagggc tatctcttct cccgcccgca agaccccgtc
     3181 gaaaccgaac gccgcctgcg cgcacaggga ctcaacgccc ccatcgacgc acccctcaca
     3241 ggcgaactga ccgccgcatg aaagggatga gggatgaggg atgagggatg aataagaatg
     3301 cttaactaac attgagcatt ctcttattca tccctcatcc ct
//
LOCUS       DASWNS010000288         7176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_37572, whole genome shotgun sequence.
ACCESSION   DASWNS010000288 DASWNS010000000
VERSION     DASWNS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37572"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VGB76_10180"
     CDS             <1..883
                     /locus_tag="VGB76_10180"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HEY0099305.1"
                     /translation="FSAQKRRANNPSPAMLPAPVGFREVEGRGLLAKVWVNNSGAYTF
                     AIDTGAGATIISKHVAAESRVALAGARSVSLSGLSGVQGSAGREATLDSLAIGARENL
                     LPGKGFVIVTDALPPDLDGVLDPTESYWPLGYTIDLPNDTISAFDPRTTPLRGLAEPP
                     GGAIVQWFFDGTSRRPFVALDVGRRALLDTGSGFGLAVSEQSARAFGILSARGRERAG
                     VTDLGRGRVNARRVEPVTIRIGSLVLRRVPTDLLTGAANAAPVLLGRDALRPFEISFD
                     PLSRLIRLSPGDVSARR"
     gene            1152..1718
                     /locus_tag="VGB76_10185"
     CDS             1152..1718
                     /locus_tag="VGB76_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0099306.1"
                     /translation="MNRAAVYIPEKLFFKIGEVCDITGVQAHVLRYWESEFPMLAPQK
                     NRAGQRTYRKRDVEMVLRIKELLYEDQYTIAGAKKKLVSEIRGASKLKVVTPDMNASS
                     VAAATEETPKETNIRDAAQLQTPAASAPPAMLAAPSYASDDEAVPVEPQMPLTDERRA
                     ALRRLSEQMRELLSLLDASERKSDPRRK"
     gene            1797..1870
                     /locus_tag="VGB76_10190"
     tRNA            1797..1870
                     /locus_tag="VGB76_10190"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1831..1833,aa:Pro,seq:ggg)
     gene            complement(2094..2522)
                     /locus_tag="VGB76_10195"
     CDS             complement(2094..2522)
                     /locus_tag="VGB76_10195"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY0099307.1"
                     /translation="MNDVTVISRTNLQNEVRFGEGQTFITDEPLSLGGDGAGADPYTL
                     LLAALGSCISMTLTLYARRKNWPLEGVEVRLRQQRIHASDCAECETTADGFVQHIERS
                     VHLEGALLTGEQRTRLQEIAHKCPVHKTLTSKIVISDMEG"
     gene            2740..3909
                     /gene="sucC"
                     /locus_tag="VGB76_10200"
     CDS             2740..3909
                     /gene="sucC"
                     /locus_tag="VGB76_10200"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY0099308.1"
                     /translation="MKIHEYQGKELLRKFGVPVPRGIVAHTPEEAFNAAKELGTDVVV
                     VKAQIHAGGRGKGGGVKLARSPEEARDIAAQMLGMQLVTHQTGPEGRKVNVLLIEEGL
                     PIDQEFYLGIVLDRASGRPVFMASQAGGMDIEEVAAHTPEKILKETIDPAVGFRPFQA
                     RKLAFGLGLPADLIGQAAKFMQALYKANEQLDASLMEINPFLLTKDKRLIALDAKVNF
                     DDNAMFRHKEFADLRDLNEEEPLEIEASKFDLNYIKLDGNIACMVNGAGLAMATMDII
                     KLAGGEPANFLDVGGGASQERVEAAFKILLADENVEAVLINIFGGIVRCDMVARGVVG
                     AAQNLGLKLPVVVRLEGTNVEEGQRVIRESGLNFIVAKGMQDAAEKVVAAAKQAA"
     gene            4023..4457
                     /locus_tag="VGB76_10205"
     CDS             4023..4457
                     /locus_tag="VGB76_10205"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEY0099309.1"
                     /translation="MRRAFNILVLLSRFGLAAMWLFAAGAKLYTLQEFTFNMTNLVPE
                     RWALPITITVIALEVVAAVLLVIPRTARSGAILTALMLVGFALYGLYYVYILKGEPLE
                     CGCFGGIIGSQLGVKTALRNLLLLVPAAIVIFGARRRVRQFE"
     gene            4573..5010
                     /locus_tag="VGB76_10210"
     CDS             4573..5010
                     /locus_tag="VGB76_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099310.1"
                     /translation="MVSKISMPVLAVCLVALIVCGAQAKVRGRKTVRLNQNFVLRVGQ
                     EATVAEQKLNVKFVSVLEDSRCPKGVNCIWAGNVRVQLQVSKARSKPAKVELSLNPRD
                     APDGEASNYGDYKLKLVSVEPYPVEGQQLRASDYTVTLSISKK"
     gene            5114..6520
                     /locus_tag="VGB76_10215"
     CDS             5114..6520
                     /locus_tag="VGB76_10215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0099311.1"
                     /translation="MATLSFWGGVGTVTGSKYLIEGGRGGRVLIDCGLFQGFKELRER
                     NWQDPPFDPASLDAVLITHAHIDHTGYLPRLVALGFKGPVYCSRGTAELLKIMLPDSA
                     RLQEEDAAYRNRHNLSRHTPALPLYTEDDARATLELMRPLPNTGESAEVAPGIRAGFR
                     IAGHILGSSLVLVEVAGAGADGEGRNILFSGDLGHYDQPIIRDPVSPPACDYMLVEST
                     YGDRLHDPIDPKEKLAEIINETKERGGVVLIPAFAIGRTQELVYLIRELEDERRIPVL
                     PVRVDSPMAAAATQVYSRRTEEQDEEYASILAQRRHPLRTHSMLTASSREESKRLNSE
                     RGARIIISASGMMTGGRVLHHAQRFLPDENSTIIFVGYQAAGTVGRRILDGAREVSIY
                     KNMIPVRCRVERIGGFSAHADYGEVLRWLEGMPGAPRRSFTTHGEPEAAAAMAGHIRQ
                     KFGWRVDAPQYGDVVELE"
     gene            6544..6972
                     /locus_tag="VGB76_10220"
     CDS             6544..6972
                     /locus_tag="VGB76_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099312.1"
                     /translation="MENSDMLSGRFKHLKILPLAERERRRLKESAHSETKLYTIPFSL
                     SAQPPHGWSEIFNLAWYQLSNQVPVRLHKDVLQLTCTLDLLPSVFNNLKAAVAAANER
                     YETKLRRDADELAATRLRQQETKRTAEHAVDAALDKLDYS"
BASE COUNT         1472 a         2192 c         2175 g         1337 t
ORIGIN      
        1 tttttccgcg caaaaaaggc gtgccaacaa cccgtcgccc gcaatgttgc ccgcccccgt
       61 aggcttccgc gaagtcgaag ggcgcggcct gctggctaaa gtctgggtca ataattcggg
      121 cgcttacacc ttcgccatcg acacgggcgc gggcgcgacg atcatctcga agcatgtggc
      181 cgccgaatcg agagtggcgc tggcgggcgc gcgttccgtc agtctcagcg gtttgagcgg
      241 cgtgcagggg tcggcgggga gggaagccac gcttgacagt ctcgccatcg gcgcgcgtga
      301 aaatctgctt cccggcaaag gtttcgtcat cgtgaccgac gccctgccgc cagacctcga
      361 cggcgtactc gacccgaccg agagctactg gccgctcggc tacacgattg atctgccaaa
      421 cgacaccatc agcgcattcg acccgcgcac gacgcccctg cgcggcctcg ccgaaccgcc
      481 cggcggcgca atcgtccagt ggttttttga cggcacgagc cgccgcccct tcgtcgcgct
      541 cgacgtggga cggcgcgcgc tcctcgacac aggctccggc ttcggactcg ccgtgagcga
      601 acagtccgca cgcgctttcg gcatcctttc ggcgcgcggg cgcgagcgcg caggcgtgac
      661 agacctcgga cgcgggcgcg tcaacgcgcg tcgcgtcgaa cccgtcacca tccggatcgg
      721 ctcgctggtg ctgcgccgcg ttcccacgga cttgctcaca ggcgcggcga acgctgcgcc
      781 cgtactttta ggacgcgatg ccttgcgtcc cttcgagatc agcttcgatc cgttgagtcg
      841 cctgattcgt ctcagtccgg gcgacgtatc cgcgcgcagg taaagtgaag tctcaaagac
      901 gctccgacgc cgcctcattc agaaaagcct tgaagccgac gcccgcaagc catactggcg
      961 cttcatctta attctctcaa attctccggc aaatcccttg aaaatcattg acacgcccgc
     1021 gccgcgtgag taacatggcg gcagcctcgc gtcgcactcg ataggcgatg cgccgtcaaa
     1081 cagttcaata caaagtttcc ggtgggaagt tagagagatg cttccgcgcc gcaaaggctc
     1141 tccaacaaac catgaatcga gccgccgtct acattcccga aaagctcttc ttcaagatcg
     1201 gagaagtgtg cgacatcacg ggcgtgcagg ctcatgtgct gcgctactgg gaatcggagt
     1261 ttccgatgct cgcgccgcag aagaatcgcg ccgggcagcg cacctatcgc aagcgcgacg
     1321 tggagatggt cttgcgcatc aaggaactgc tctacgaaga ccagtacacg attgcgggcg
     1381 cgaagaaaaa gttggtcagc gaaattcgcg gggcgagcaa actgaaagtc gtcacgccgg
     1441 acatgaacgc ttcgagcgtt gctgcggcaa cggaggagac tccgaaagag acgaacatac
     1501 gcgacgccgc ccaacttcag acacccgctg cgagcgcgcc gcccgccatg ctcgccgcgc
     1561 cttcttatgc gagcgacgac gaggctgtcc ccgtcgaacc gcagatgccg ctcacggacg
     1621 aacgccgcgc cgccctgcgt cgcctcagcg aacagatgcg cgaactgctc tccctgctcg
     1681 acgcctccga acgcaaaagc gacccgcgcc gcaagtagtt tctcccgccg ccgatgcgct
     1741 cgacgctccg cgccatttga caaaaataat tttccagaga tactaaagaa cacagacggg
     1801 atgtggcgca gcctggtagc gcgcaccctt ggggtgggtg aggccccgag ttcgaatctc
     1861 ggcatcccga tcacgcgaaa gggcgtccga cttatcaagt cggacgccct ttcgcttttc
     1921 cctcgtgccg ccgtcagcct caggcgcgcg ttaccttcgc cggtttgcga ccgccagcct
     1981 acgcgctccg ccctgccagt agcagaaagc gatgcgctgc gtcttcgcgt taatgctcgg
     2041 gtcgccagaa ggaataacag aagtgatgaa cggagagttc gcatggtcgc tgctcagcct
     2101 tccatatccg agatcacgat ctttgacgtg agcgttttat ggacgggaca tttgtgcgcg
     2161 atctcctgca agcgcgtgcg ttgttctccg gtgagaagcg cgccttcgag atgcacggaa
     2221 cgttcgatgt gttgaacgaa accgtcggcg gtcgtttcac actccgcgca atcgcttgca
     2281 tggatgcgtt gttgacgcag acgcacttcc acgccttcga gcggccagtt tttgcgtcgc
     2341 gcgtagagcg tgagtgtcat cgagatgcaa ctgccgagcg cggcgaggag caaggtgtaa
     2401 gggtctgctc ccgcgccgtc gccgccgagg ctcaggggtt cgtccgtgat aaaagtttgc
     2461 ccttctccga agcgcacctc attctggaga ttcgtgcggg atatgaccgt gacatcgttc
     2521 atggaaacgt ctcctgcgcg ggtgtgttga cggcaaagcg aaatttgcgt cgagggtgat
     2581 tctcagagtc gcgggcatca taacaaattt gcgaaagcgc ggacgggtgg aatagtggct
     2641 tgcggtcgtg ggatgcgtgc ggctaaactc cagagcgttc cacgacaaag aaaattcagc
     2701 ggcggcttca ggttcgccgg acgagcgaga ggatcgagga tgaagattca cgagtatcaa
     2761 ggcaaagagt tgttgagaaa attcggcgtg cctgtcccgc gcgggatcgt ggcgcacacg
     2821 cccgaagagg ctttcaacgc ggcgaaggaa ctcggcacgg acgttgttgt cgttaaagcg
     2881 caaattcacg cgggcgggcg cggcaagggc ggcggcgtca aactcgcgcg gagtcccgaa
     2941 gaggcgcgcg acatcgccgc gcagatgctc ggcatgcaac tcgtcacgca ccagacgggt
     3001 cccgaaggac gcaaggtaaa cgtgctgctc atcgaagagg gcttaccgat tgatcaggag
     3061 ttttatctcg gcatcgtgtt ggatcgtgcg tcggggcgac ccgtctttat ggcgtcgcag
     3121 gcgggcggca tggacatcga agaggtggcg gcgcacacgc ccgaaaaaat tctcaaagag
     3181 acgattgatc ccgccgtcgg ctttcgcccg ttccaggcgc gcaaactcgc cttcggactt
     3241 ggcctgcccg ccgatctgat cggtcaggcc gcgaagttca tgcaggctct ctacaaagcc
     3301 aacgagcagt tggacgcgtc gctcatggag atcaacccgt tcctcctgac gaaagacaaa
     3361 cgcctcatcg cgctcgacgc gaaagtcaac ttcgacgaca acgcgatgtt tcgtcacaag
     3421 gagtttgcag acctgcgcga cctgaacgag gaagagccgc tcgaaatcga agcatccaag
     3481 tttgatctca actacatcaa acttgacggg aacatcgcct gcatggttaa cggcgcgggg
     3541 ctggcgatgg cgacgatgga cattatcaaa ctggcgggcg gcgaacccgc caacttcctc
     3601 gacgtgggcg gcggcgcatc gcaggaacgc gtggaagcgg ccttcaagat tctactcgca
     3661 gacgaaaacg tcgaggccgt gctcatcaac atcttcggcg gcatcgtgcg ttgcgacatg
     3721 gtggcgcgcg gcgtggtcgg tgcggcgcag aatctgggct tgaagttgcc cgtcgtcgtc
     3781 cgcctcgaag gcacgaacgt cgaagaaggc cagcgggtga tacgcgaatc cggcctcaac
     3841 ttcatcgtcg ccaaaggcat gcaggacgcc gccgagaaag tcgtcgcggc ggcgaagcag
     3901 gcagcctgac atagagggat gaaggatgag ggatgaggga tgaataaaac caaagttcgc
     3961 caacagtttt ggatgcttct cttattcatc cctcatccct catccctcat cctttaattc
     4021 ttatgcgtag agcgttcaac attctcgtcc tcctcagtcg cttcgggttg gcggcgatgt
     4081 ggttgtttgc ggcgggcgcg aagttatata cgctgcaaga gttcacgttc aacatgacga
     4141 acctcgtgcc ggagcgttgg gctttgccca tcacgatcac cgtgatcgct ttggaagtgg
     4201 tcgccgccgt gctgctagtc atcccgcgca cggcgcgctc cggggcgatt ctgacggcgt
     4261 tgatgctcgt cggcttcgcg ctctacggtc tgtactacgt ctatattttg aagggcgagc
     4321 cgctcgaatg cggttgcttc ggcggcatca tcggcagcca actcggggtg aagaccgcgc
     4381 tccggaatct cttgttgctc gtgcccgccg ctatcgtcat tttcggcgca cggcgacgcg
     4441 tgcgacagtt tgaatgagtg ccaaaagcag tgacgagtga caagttaaat cagaaagctc
     4501 gtctttaaac ttgtctttta acctcatcac ttgtcactcg tcactcgcca ctgttttggg
     4561 agggctttac aaatggtttc gaaaataagt atgccggtct tggccgtgtg tctcgtcgcg
     4621 ttgatcgtgt gcggagcgca ggcgaaagtg agagggcgaa aaaccgtgcg gctgaaccaa
     4681 aattttgtgt tgcgggtggg acaagaggcg acggtcgcgg agcagaaact gaacgtgaaa
     4741 ttcgtctccg tgctggaaga ctcgcgttgt ccgaagggcg tgaactgcat ctgggcgggc
     4801 aacgtgcgcg tccaactgca agtgtcgaag gccaggagca agccggccaa agtcgaactc
     4861 agcctcaacc cgcgagacgc tccggacggc gaagcctcca actacggcga ctataaactc
     4921 aaactcgtca gcgttgagcc ttaccccgtc gaaggccagc aactccgcgc cagtgactac
     4981 accgtgacgt tgagcatctc caaaaaatag tctccgcctc cgtctctcta ttgaaacgca
     5041 gcgcggctta aattcagcac ggcacaattc agcgcggcgc cattcgggac ggctcaggca
     5101 gaggggagca tagatggcga cactttcttt ctggggcgga gtcggcacgg tcaccggctc
     5161 gaagtatttg attgaaggtg ggcgcggcgg gcgcgtgctc atcgattgcg ggctgtttca
     5221 aggcttcaaa gaactgcgcg agcgcaactg gcaagacccg cccttcgatc ccgcgagcct
     5281 cgacgcagtt ctcatcacac acgcacacat cgaccacacg ggctacctgc cgcgtctcgt
     5341 cgcgctcggt ttcaaaggac ctgtctattg ttcgcgcggc accgccgaac tcctcaagat
     5401 catgctgccc gattccgcgc gcttgcagga agaggatgcc gcctatcgca accgacacaa
     5461 cctctcgcgt cacacaccag ccctcccgct ctacaccgaa gacgacgcgc gtgcgacgct
     5521 cgaactgatg cgacctttgc cgaacacggg cgagtcggcg gaagtcgcgc cggggatccg
     5581 cgcgggcttt cgtatcgccg gacacatcct cggatcgagt ctcgtgctcg tcgaagtcgc
     5641 aggcgcgggc gcagacggcg aagggcgcaa catccttttc tcaggcgacc tcggccacta
     5701 cgaccagccg atcatacgcg accccgtctc gccgcccgcc tgcgactaca tgctcgtcga
     5761 atcaacctac ggcgaccgcc tgcacgaccc catcgacccg aaagagaaac tcgccgagat
     5821 catcaacgag acgaaagagc gtggcggcgt ggtgctcatt ccggccttcg ccatcgggcg
     5881 cacgcaggag ttggtctatc tcatccgcga actcgaagac gagcggcgga ttccggttct
     5941 gcccgtgcgc gtagattcgc cgatggccgc cgccgcgacg caggtgtaca gccgccgcac
     6001 ggaagagcag gacgaagagt atgccagcat tctcgcgcag cgtcgccacc ctttgcgcac
     6061 gcattcgatg ttgacggcct cttcgcgcga agagtcgaag cgtctgaact cggagcgcgg
     6121 cgcgcgcatc atcatctcgg cttcgggcat gatgacgggc gggcgcgtcc tgcatcacgc
     6181 gcagcgattt ctgcccgacg aaaactcgac catcatcttc gtcggctatc aggcggcggg
     6241 aaccgtcggc agaagaattc tcgacggcgc acgcgaagtc agtatctaca agaacatgat
     6301 cccggtgcgc tgtcgcgtcg aacgcatcgg cggcttctct gcgcacgccg actacggcga
     6361 agtcctgcgc tggctcgaag gcatgcccgg tgcgccgcgt cgctccttca ccacgcacgg
     6421 cgaaccggaa gccgccgccg cgatggccgg acacatccgg cagaagttcg gctggcgagt
     6481 cgatgcgccg cagtacggcg acgtggtcga acttgagtga gtcggcggac gcgcggaagt
     6541 tgaatggaga atagcgatat gctgtcgggc aggttcaaac atctcaaaat attgccgctg
     6601 gctgagagag agagaaggag attgaaggag tcggctcata gcgagacgaa gctctacacg
     6661 atccctttta gcttatcggc gcagcccccg cacggttggt ctgaaatatt caatctcgcg
     6721 tggtatcaac tgagcaatca ggttccggtg cgtctgcaca aggatgtgct gcaactgaca
     6781 tgcacgctcg atcttctccc ctccgtcttt aacaacttaa aagccgccgt cgccgccgca
     6841 aatgaaagat acgagactaa acttcggcgt gacgcggatg aactggccgc gacgcgtctg
     6901 cggcagcagg agacgaagcg aaccgccgag cacgccgtcg atgcagccct cgacaaactg
     6961 gactattcat aaaagtcagc gtcactttcc gcactttccg ctgcctcggc aacgtcgggg
     7021 cgtgaccggc aacccggcct gcgcctgtcg ttgccggatc gcgcggcggc tcacggcaga
     7081 aacagtccgc gccggaagtt gacggcaaag ccgcagcgtc gggcgcgacc ggggcggcgt
     7141 cgaagcaggg cgcgcgacac gctgtacagc gtgtcg
//
LOCUS       DASWNS010000289         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_12441, whole genome shotgun sequence.
ACCESSION   DASWNS010000289 DASWNS010000000
VERSION     DASWNS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12441"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VGB76_10225"
     CDS             complement(<1..619)
                     /locus_tag="VGB76_10225"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0099313.1"
                     /translation="MENIERTQFVVLTGHLDDQPLPDLVRQLRVQRKSGRLQVEYADA
                     PGSFFFEDGQLVDAQLGNLRGLEALYAALALQGAAFNFNPLVRPPERSIDRQQQKFIS
                     DLVETPRRENLAEIKIAGNAFPANANAIATSAAAPAPLQLAPVHAELIAPLEERLTAV
                     EEAIDATSRRFSRERLIYAIVISFLVGLTAVTALQALLNLPGPIPS"
     gene            complement(674..>1733)
                     /locus_tag="VGB76_10230"
     CDS             complement(674..>1733)
                     /locus_tag="VGB76_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099314.1"
                     /translation="ADFEAKRNLNARTLVPLAVKTPSMICRMLKSLLSIVILLCGVCL
                     VVCFEARGQGNRSRSENNRREDRTPVVFLDSTKEADGLKGAVRRVETEIVRVQLEGGK
                     LLEKSRSLLERTLYDERGRRVENETYPIVGGQTGQEAHKYDAQGNLSETVVRDARGAV
                     LSRTIYNYELDQFGNWIQMTASVAASGQTGYEPFEITKRNITYYHVDEPGATDASAAS
                     RESATPAPVAEGDRVSRRVESSAPKKPQAVLPDTTAAEDASILNVGVLNKRTIELPKP
                     ASPVSWKRLDAPITVTVEVVVDITGRVVEARAQNAPEALLEPSESAARRATFLPFYVA
                     GRPVKARGLINYTFDYLP"
BASE COUNT          316 a          535 c          537 g          345 t
ORIGIN      
        1 cagacggaat agggccggga agattcaaca atgcttgaag ggctgtcacc gccgtcaggc
       61 cgacgaggaa gctgatgacg atagcgtaga tcagacgttc gcgtgagaag cggcgcgagg
      121 tggcgtcgat ggcctcttcc accgccgtca gacgctcttc gagcggggcg atcaattcgg
      181 cgtggacggg cgcgagttgg agcggcgcgg gcgcagccgc actggtggct atcgcgttcg
      241 cgtttgcggg aaaggcgttg cccgcgatct tgatctccgc cagattttcg cgtcgcggcg
      301 tttccacgag gtcggagata aacttctgct gctggcggtc gatgcttcgt tcgggcgggc
      361 ggacgagcgg gttgaagttg aaggctgcgc cttgcagcgc gagcgcggcg tagagagctt
      421 cgagtccgcg caggtttccg agttgggcat cgacgagttg cccgtcctcg aagaagaacg
      481 agccgggcgc gtccgcgtat tcgacctgta gccgcccgga ctttctctgc acgcgcagtt
      541 gcctgacgag atcaggcaac ggctggtcgt ccagatgtcc ggttaaaacc acgaactgcg
      601 tgcgctctat attttccaat tcgccaattc cccgaacctg aaaatctcgc cgtcgaaagc
      661 gtcgcccgcc tgcttacggc aagtaatcga aagtgtaatt gatcaaaccg cgcgctttga
      721 ccggacgccc ggccacgtag aaaggaagaa acgtcgcgcg ccgcgctgcg ctctccgacg
      781 gttcgagcag agcctcgggc gcgttctgcg cacgcgcctc cacgacgcgc cccgtgatgt
      841 ctacaaccac ctcgaccgtc accgtgatgg gcgcatcgag ccgcttccaa ctcacaggcg
      901 acgcgggctt aggcaactca atcgtccgtt tgttgagcac gccgacattc aatatcgaag
      961 catcttctgc ggcggtcgtg tccggcagga cggcctgcgg ctttttcggg gcagaacttt
     1021 cgacgcggcg actcaccctg tcgccctccg cgacgggcgc aggcgttgcg ctttcgcgcg
     1081 acgcggcgga ggcgtccgtc gcacccggtt catcaacgtg gtaataggtg atatttcgtt
     1141 tcgtgatttc gaagggttca tacccggtct ggccggaggc ggcgaccgaa gccgtcatct
     1201 gaatccagtt gccgaattga tccagttcgt agttatagat cgtccggctc aacacagccc
     1261 cgcgcgcatc gcgcacgacc gtctcggata gattgccctg cgcatcgtat ttgtgcgcct
     1321 cctgcccggt ctggccgccg actatcgggt aagtctcatt ttcgacgcgc cgcccgcgct
     1381 cgtcatagag cgtccgctcc aacagcgagc gcgacttctc caggagtttg ccgccctcaa
     1441 gttgcacgcg gacgatctcc gtctcgacgc gccggacagc acctttgagt ccgtccgcct
     1501 ccttcgtgct gtcgagaaag acgacgggcg tgcgatcttc acggcggttg ttttcagagc
     1561 gtgaacgatt gccctgccct ctcgcctcaa agcagacgac aaggcagacc ccgcagagca
     1621 ggatgacgat ggacaagagc gactttaaca tccggcaaat catggaaggg gttttcactg
     1681 cgagcggcac gagcgttcgg gcattcaagt tgcgcttcgc ctcgaagtcc gcc
//
LOCUS       DASWNS010000290         6439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_37657, whole genome shotgun sequence.
ACCESSION   DASWNS010000290 DASWNS010000000
VERSION     DASWNS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37657"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VGB76_10235"
     CDS             <1..741
                     /locus_tag="VGB76_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY0099315.1"
                     /translation="PTYIRSHIHPDTLADDQTVLISTFRLPVQIHTRSANAKWLDEIA
                     HTNPLWIHTSHAARLGVRTGDLVRVETEIGYFVVRAWVTEGIKPGIVACSHHMGRWKL
                     KEQGQRQMMATVSLAHEANRWGLKRESALAPYESADADTQRIWWTDVGVHQNLTFPVH
                     PDPISGMHCWHQAVRVRRAEATDAHGDISVDTEKSHEVYLQWLARTRSAEKFSPDGTR
                     RPYWLLRPLKPAREFYRLPDEPELVSVE"
     gene            741..1619
                     /locus_tag="VGB76_10240"
     CDS             741..1619
                     /locus_tag="VGB76_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEY0099316.1"
                     /translation="MELFRALAVLAEPPVPESARIAHALELGALPDASEYTEVFVFQL
                     YPYASVYLGAEGMLGGEARDLIAGFWRALQQTPPAEPDHLSVMLALYAELQTLEANEP
                     DDAPRANWRRARRAFLWEHLLSWLPAYLSKLKEIAPPFYRRWGDVLLDALLEEACANG
                     AQAQLSLHLRERASGLVDPREGETGEFLQTLLSPARSGMILVRSDLTRAARQLELGLR
                     VGERKYVLESLLAQDARGVLGWLAREARAWSERHREQQDALGVNAHAWEAKATAAAEL
                     LEELEAAAAEVRNDEG"
     gene            complement(1710..3161)
                     /locus_tag="VGB76_10245"
     CDS             complement(1710..3161)
                     /locus_tag="VGB76_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEY0099317.1"
                     /translation="MTDLDRADIQGIVLSAYGHLTCAAYLMLRVREARGARGWLASVA
                     GEITNASGKQEGFSLNLALTYEGLQAIGLDPDALATFPIPFSDGMASPKRSHILGDTN
                     RDAPANWDWGSEKNPVHILLLVFGADEDALAAQLKLRREELARFGGVEEVLQLSAGRQ
                     PDSHEHFGFNDGIAQPVIEGGGRRQRQLRRTGHATEIKPGEFVLGYVNEYDIPADCPT
                     VDPARDPQELLHPYEYPPQLLSENAPPPPRRNLGINGSYLVFRQLEQHVPAFWNFVAE
                     AAREIYGAERGAASVEHLAAKFVGRWPSGAPLVKSPDRDDPQLATDNSFGYAATDREG
                     LACPIGAHIRRSNPRDSLGDKPGAALSAARRHRLMRRGRSYGHRIADKYTDDGQQRGL
                     HFICLNSELDRQFEFVQQTWLNNPVFGGLYNETDPLVGNQDKDKCDGLFTIPGAPVRV
                     RVHDLCSFLRVRGGAYFFLPGLRALRYLGSLTG"
     gene            complement(3178..4452)
                     /locus_tag="VGB76_10250"
     CDS             complement(3178..4452)
                     /locus_tag="VGB76_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099318.1"
                     /translation="MTHTILNFITTVDPAKLEPLTALLAEIEDDLSGNPHLPFSCLKR
                     LHFGSLTILEDTGDEHGRKNFAPYLVFEHNFDGTLEPYLEELLDCAAPGLDRIYSHCL
                     EYPASGLRERATLRAFLISRVVRPHAYHIGNVGRSVGRVQQEAALREQIEGHLDALVL
                     KGQAQAPPATIRRGIQEFVRSLAPDWTQTVAPRMTFTERFVPWVKLVAAAFLVLLLAL
                     VLFPLFIIYLIALRRRERTDPANIPPLDPAHVLGLAAREDHIVQNHMANLRYVKPGSL
                     RRLTIRFVLLAVNLVARVSTHGQLVGLRSLHFAHWVVIDDGRRLLFLTNYDGSWENYL
                     DDFIDRIAIGLTAIWSNTINFPRSRFLLFGGAKDGARFKATARQTQFYTNVWYSAYPR
                     LTVQAVDNNSAIREDLFTPLGEAQTRAWLRRF"
     gene            4698..5252
                     /locus_tag="VGB76_10255"
     CDS             4698..5252
                     /locus_tag="VGB76_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0099319.1"
                     /translation="MASEEDEFAVMFNEFYPSLCRFIACLLGGRGASGMAQEIAQESF
                     LQLHRTGLRSLPEGEARFWLYRVARNFALNELNRHNTRHRLVGRVVDVFRAHVPTPEE
                     EFEMAERKGLVIDMLKTLPEQQRAALLLREQEELSYREIAGVLNVSESKVKVDVFRAR
                     TALRERWRKQQGALAHSGSRSQAG"
     gene            5288..>6439
                     /locus_tag="VGB76_10260"
     CDS             5288..>6439
                     /locus_tag="VGB76_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0099320.1"
                     /translation="MKCVWTQKVSLLIDGELDRAESREVEQHLDACGDCREAQADFLS
                     LRRQLAAYQVDLDAFAAQRALKRILASGASGATREGGRNDMSSRRGEGRFAGVFGMPR
                     LSPVGLGALLLLLVGVAAGIVSLINTRRPAVEVAKTTPTPTPQNEVRNNSNGNAQPAP
                     EIIPGAGTTGTKEGGETMAGNNRKEFGEASTKNGGLSAANARTRGRLPRTASAGDKVK
                     KSSPSLNRVPSDLPFAETARNNGAVVEGGVETLETSDAGDDLTARRGREAAGDSKTAR
                     HVEQAQLLLRSFRNARLSDAGRRASSDFAYDKRQSKRLLYQNIVLRREAASRGNLPVE
                     SLLDSLEPILIDIANLPDKPAPEDVREINERMRRKNLVAMLQISSVESAS"
BASE COUNT         1173 a         1903 c         2214 g         1149 t
ORIGIN      
        1 ccgacttaca tacgcagcca cattcatccg gacacattag ctgacgatca gacagttctg
       61 atctcaacct tccgcctgcc cgtgcagata cacacgcgct ccgccaacgc caagtggctc
      121 gacgagatcg cgcacacgaa ccctctgtgg atacacacgt cgcacgccgc gcgcctcggc
      181 gtgcggacgg gcgacctcgt gcgcgtcgag acggagatcg gctatttcgt cgtgcgcgcg
      241 tgggtgacgg agggcatcaa gccgggcatc gtcgcgtgca gccatcacat ggggcgttgg
      301 aagttgaagg aacagggtca gcggcagatg atggcaaccg tctcgctcgc gcatgaggcg
      361 aaccgctggg gcttgaaacg cgaaagcgcg ctcgcgcctt acgagtcggc ggacgcagac
      421 acgcagcgca tctggtggac tgacgtgggc gtgcatcaaa acttaacttt tcccgtccat
      481 cccgatccca tctccggaat gcactgctgg catcaagccg tgcgcgtcag gcgtgcggag
      541 gcgacggacg cgcacggcga catctcggtg gatacggaga agtcgcacga ggtctatttg
      601 cagtggctcg cgcggacgcg ttcggcggaa aagttttcgc ccgacggcac gcgccgcccc
      661 tactggctcc tgcgcccgct caagcccgca cgcgaattct atcgcctgcc ggatgagccg
      721 gaactggtga gcgtcgagtg atggaactgt ttcgcgcgct cgccgtgctc gcagagcctc
      781 ccgtgccgga gagcgcgcgc atcgcacatg cgctcgaact cggcgcgctc cccgatgcga
      841 gcgagtacac ggaagttttc gtcttccaac tctatcctta cgcctctgtc tatctcggcg
      901 cggagggaat gctcggcggc gaggcgcgcg atctgatcgc gggcttctgg cgcgcgctgc
      961 aacagacgcc gcccgccgaa cccgatcacc tctccgtgat gctcgccctc tacgccgaac
     1021 tccagacact cgaagccaat gaaccggacg acgcgccgcg cgcgaactgg cgacgcgcac
     1081 ggagggcttt tctgtgggag cacctcttga gttggctgcc cgcctatctc tccaagttga
     1141 aggagatcgc gccgccgttt tatcgccgct ggggagacgt gcttctcgac gcgctgctgg
     1201 aagaagcgtg cgcaaacggg gcacaggcgc agctctcttt gcacctgcgc gaacgcgcgt
     1261 cgggactcgt cgatccgaga gagggggaga cgggagagtt tttgcagacg cttctgagtc
     1321 cggcgcgcag cgggatgatc ctcgtgcgtt ccgatctcac acgcgcggcg cgtcaactcg
     1381 aactcggcct gcgcgtcggc gaacgcaagt acgtgctcga atctctactg gcgcaggacg
     1441 ctcgcggcgt gctcggctgg ctcgcgcgtg aggcgcgcgc gtggagcgaa cggcaccggg
     1501 agcagcagga cgcgctcggc gtgaacgcgc acgcgtggga agcaaaggcc accgccgccg
     1561 cagagttgtt ggaagaactc gaagccgcag cggcagaagt aagaaacgat gaaggatgaa
     1621 ggatgaagga tgaaggatga acgatgaaag cagtggctcg cgggtctgct ttcattcatc
     1681 gttcatcgtc catcgttttc cctaagggct tagcccgtca ggctgccgag ataacgcagg
     1741 gcgcggaggc cggggaggaa gaagtatgcg ccgccgcgca cgcggaggaa actgcacagg
     1801 tcgtgaacgc gcacgcgcac cggcgcgccg gggatggtga agaggccgtc gcatttatcc
     1861 ttatcctgat tgccgacgag cgggtcagtc tcgttgtaga gtccgccgaa gacggggttg
     1921 ttgagccagg tctgctgcac aaactcgaac tgccggtcta gttcgctgtt gaggcagatg
     1981 aagtgcaggc cgcgctgctg cccgtcgtcc gtgtacttgt ctgcgatgcg atggccgtag
     2041 gagcgaccgc gccgcatgag acggtggcgg cgggctgcgc tcaaggccgc gccgggcttg
     2101 tcgccgaggg agtcgcgcgg gttggagcgg cggatgtgcg cgccgatggg acaggccaga
     2161 ccttcgcggt cggtcgcggc gtagccgaag ctgttgtcgg tcgcaagttg cgggtcgtcc
     2221 cggtcgggcg acttgacgag gggcgcgccg ctcggccaac gccccacaaa cttcgcggcg
     2281 agatgctcga ccgatgccgc gccgcgctcc gcgccgtaaa tttcccgcgc cgcttcggcg
     2341 acgaaattcc agaaggccgg aacgtgctgt tcgagttggc ggaagacgag atagctgccg
     2401 ttgatgccga ggttgcggcg gggcgggggc ggcgcgtttt cgctgagcag ttgcggcggg
     2461 tattcgtagg gatgcagcag ttcttgcgga tcgcgtgcag ggtcaacggt cgggcagtct
     2521 gcgggaatgt cgtactcgtt gacgtagccg agcacgaact cgccgggttt gatttcggtg
     2581 gcatggccgg tgcgacgtag ttgtctctgt ctgcgcccgc cgccctctat gacgggctgc
     2641 gcgatgccgt cgttgaaacc gaaatgctcg tggctgtcgg gctggcgacc cgccgaaagc
     2701 tgcaagacct cttcgacgcc gccgaagcgc gccaactcct cgcgccgcag tttgagttga
     2761 gcggcgagcg cgtcctcgtc cgcgccgaag accagcagga ggatgtggac gggattcttc
     2821 tcgctgcccc aatcccagtt tgcgggcgca tcccggttgg tgtcgccgag gatgtgggag
     2881 cgtttgggcg aagccatgcc gtcgctgaag gggatgggga aggtggcgag cgcgtcgggg
     2941 tcgagtccga ttgcttgcag cccttcgtag gtcagcgcga ggttgagcga gaagccttcc
     3001 tgtttgccgc tcgcgttcgt gatctctccg gcgacggagg ccagccagcc gcgcgcgcca
     3061 cgcgcttcgc ggacgcggag catgaggtaa gcggcgcagg tcaggtggcc gtatgcgctc
     3121 agcacgatgc cttgaatatc cgccctgtct aaatctgtca aggtcgtctc cttccggtta
     3181 aaagcggcgc agccaagccc tcgtctgcgc ctcgccgagc ggcgtgaaga ggtcttcgcg
     3241 gatcgcgctg ttgttgtcaa ccgcctgcac agtcagacgc gggtatgcgc tgtaccagac
     3301 gttcgtataa aactgcgtct ggcgcgcggt ggccttgaag cgcgccccgt ccttcgcccc
     3361 gccgaagagt aaaaacctgc tgcgcgggaa gttgatcgtg ttgctccaga tggcggtcag
     3421 gccgatggcg atccggtcga tgaagtcgtc gaggtagttc tcccaactgc cgtcgtagtt
     3481 ggtgagaaac aggagccgtc gcccgtcgtc gatcacaacc cagtgagcga agtgcaggct
     3541 gcgcaggccg accagttggc cgtgcgtcga gacgcgcgcg acgaggttta ccgccagcaa
     3601 cacgaacctg atcgtgagac ggcgcaaact ccccggcttg acgtagcgga ggtttgccat
     3661 gtggttctgc acgatgtggt cttcgcgcgc ggcgagtccg agcacgtgcg cgggatcgag
     3721 cggcgggatg ttcgccgggt ctgtccgctc gcgccgtcgc agcgcgatga gatagatgat
     3781 gaacagagga aagagcacga gggcgagcag gaggacgagg aaggccgccg cgacgagttt
     3841 cacccagggc acgaaccgct ccgtgaaggt catgcgcggc gcgaccgttt gagtccaatc
     3901 aggagcgaga ctgcggacga actcctgtat gccccggcgg atcgttgcgg gcggggcttg
     3961 cgcctgacct ttgagcacga gtgcgtcgag atggccttcg atctgttcgc gcaaggccgc
     4021 ctcctgctgc acgcgcccga cgctgcgccc gacgttgccg atgtgataag cgtgcgggcg
     4081 tacgacgcgc gagataagga aggcgcgcag cgtcgcccgt tcgcggaggc cgctcgcggg
     4141 atattcgagg caatggctgt agatgcggtc gaggccgggc gcggcgcagt cgagcagttc
     4201 ttcgagataa ggttcgagcg tcccgtcgaa gttgtgttcg aagacgaggt agggagcgaa
     4261 atttttgcgc ccatgctcgt cgccggtgtc ttccaggatc gtgaggctgc cgaagtgcag
     4321 acgcttgaga caggagaagg gcaggtgcgg gttgccgctc aggtcgtctt caatctctgc
     4381 gagcagggca gtcagcggtt cgagtttggc cggatcgacg gtggtgatga agttgagaat
     4441 cgtgtgggtc atggcgtcct cggcgggagc gtgagttgat aatgcgcggc tggccttttt
     4501 gcgtgcgcgg ctggctaacg tgcgggcgcg cgaatcatat agagactcag ggggcgggtc
     4561 aatcacaaat ttctacggcg cgggaaatgg ccgggcggga cgtgtgcgtc gaaccggcgg
     4621 gcgcgtcgct ctgacgccgc gaaaaatatt tgtaactttt cccgtccccg gctgttttag
     4681 gggcgtacag tgtttttatg gcgagcgaag aagacgagtt cgcggtgatg ttcaacgagt
     4741 tctaccccag cctgtgccgt tttatcgcat gcctgttggg agggcgcggc gcatcgggca
     4801 tggcgcagga gatcgcgcag gagagtttct tgcagttgca tcgcacgggc ttgcgaagtt
     4861 tgccggaagg cgaggcgcgt ttctggctct accgcgtggc gcgcaacttc gcactcaacg
     4921 agttgaaccg gcacaacacg cgccatcgtc tcgtcggtcg tgtagttgat gtttttcgcg
     4981 ctcacgtgcc gacgccggaa gaggagtttg agatggccga gcgcaaaggc ttagtgatcg
     5041 acatgctgaa gaccttgccc gaacagcagc gcgcggcctt gctcctgcgc gagcaggagg
     5101 agttgagcta ccgcgagatc gcgggggtgc tcaacgtctc ggagagcaaa gtcaaagttg
     5161 atgtctttcg cgcgcgcacg gctttgcgcg agcgttggcg caaacagcag ggagcgttgg
     5221 cgcacagtgg tagtagaagc caggccggat gaaagtttga acggatgagc cgcgagcagt
     5281 gacgactatg aaatgcgtct ggacacaaaa agtttcgctc ttgattgacg gcgaactcga
     5341 ccgcgccgag tcgcgcgagg tggaacaaca tctcgacgcc tgcggcgact gtcgtgaggc
     5401 gcaggccgat tttctctccc tgcggcgaca actcgccgct taccaggtag acctcgacgc
     5461 tttcgccgca cagcgcgcgc tcaaacgcat tctcgcctcg ggcgcgagcg gcgcgacacg
     5521 cgaaggcggc aggaatgata tgagttcgcg tcgcggcgaa ggacggttcg cgggcgtgtt
     5581 cggcatgccg cgcctgagtc ccgtcggatt gggcgcgttg ttgctgctcc tcgtcggcgt
     5641 cgccgcaggc atcgtctctc tcattaacac gcgccgcccg gcggtcgaag tggcgaagac
     5701 gacgccgacg cccacgcctc agaacgaggt gcgcaacaac tcgaacggca acgctcaacc
     5761 cgcgccggag ataattcccg gagccggaac gacgggcacg aaagagggcg gggaaacgat
     5821 ggccggaaat aatcggaagg agtttggcga agcgtcaacg aaaaacggcg gcctgagcgc
     5881 agcgaacgcg cgcacgcgcg gacgccttcc acgcaccgcg agtgcgggcg acaaagttaa
     5941 aaagtcgtct ccgtccctga accgcgtgcc gtctgatctg cccttcgcgg agacggcgag
     6001 aaataacggc gcggttgttg aaggcggcgt cgagacgctt gagacgagcg acgcgggcga
     6061 cgaccttacg gcgcggcgcg ggcgagaggc ggcgggcgac tcgaagaccg cccgccacgt
     6121 cgaacaggcg cagttgctcc tgcgttcgtt ccgcaacgcg cgcctgtcgg atgcaggtcg
     6181 gcgcgcttcg tcagatttcg cctacgataa acgccaatcg aagcgactgc tctaccagaa
     6241 catagtgctc cggcgcgaag cggcgagtcg cggcaatctc cccgtcgaat cgctgctcga
     6301 tagtcttgag ccgatcctca tagacatcgc caacttgccc gacaagcccg cgcccgaaga
     6361 cgtgagagag atcaacgaac gcatgcgccg taagaacctc gtcgccatgc tccagatcag
     6421 ttcggtcgaa tccgcctcc
//
LOCUS       DASWNS010000291         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_220463, whole genome shotgun sequence.
ACCESSION   DASWNS010000291 DASWNS010000000
VERSION     DASWNS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220463"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VGB76_10265"
     CDS             <1..526
                     /locus_tag="VGB76_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0099321.1"
                     /translation="ITEMMTATSVERQKRFVGLHFFNPVPIMKLVEVVKTILTDDAVY
                     ETAVDFGKRLGKVPVRASDKTGFIVNRLLVPYMLDSIRALEEGVGSIVDIDNAMKLGC
                     GYPMGPLTLGDFVGLDTTYYIAEIMFNEFREKRFAPPPLLKRMVLAGLYGRKSGRGFY
                     DYADPKNPVPMNLL"
     gene            630..1508
                     /locus_tag="VGB76_10270"
     CDS             630..1508
                     /locus_tag="VGB76_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0099322.1"
                     /translation="MSKEQQLVLASAAVGAASDFGSGSIFFVGTATVILRYAGFTILT
                     DPNFLHAGDHVHLGYGLTSERLTNPAIELEELPPIDFCVLSHYHGDHFDRIVEERLQK
                     DLPIITTEHAAGELKSKGFTAPLALDTWDAVTVERGDASVRITSMPGKHGPTAVDFFL
                     PPVMGSMLEFRNAAGQRTTLRLYITGDTLVHDDLEEIPKRYPEIDLALFHLGGTRIMG
                     ILLTMDAEQGVEAIRIINPREVIPIHYNDYGVFQSPLEDFKKAVEAAGYADRVKYLSH
                     GETYDFEVPASRYAST"
     gene            1543..>2123
                     /locus_tag="VGB76_10275"
     CDS             1543..>2123
                     /locus_tag="VGB76_10275"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY0099323.1"
                     /translation="MKRNSSYALRLLLFIACLGLGSIETTQAQRQQASARYVCPMHAE
                     VTSRKPGSCPKCMMKLVARKRDGAQATTPAASSQSQSTVAAATAERVRALEQLAPSYE
                     FTCSMHPEIHQAQEGVCPKCGMPLTTVKPSVRGAYRLDVVPTPRAPKPGEALRLNFVV
                     SHPETGARVRDYVLNHERLFHLFIVSADLNEYQ"
BASE COUNT          435 a          668 c          609 g          411 t
ORIGIN      
        1 gatcacggag atgatgacgg ccacctccgt cgaacgccag aagcgtttcg tcggcctgca
       61 cttcttcaac cccgtgccga tcatgaaact cgtcgaagtc gtcaagacga tcctgacgga
      121 cgacgcggtc tatgaaacgg cggttgattt cggcaagcgg ctcggcaaag ttccggtgcg
      181 cgcgagcgac aagacgggct tcatcgtcaa tcgcctgctc gtgccttaca tgctcgattc
      241 catccgcgcg ctcgaagagg gcgtcggctc gattgtagac atcgacaacg cgatgaaact
      301 cggctgcggc taccccatgg ggccgctcac gctcggcgac ttcgtcggcc tcgacaccac
      361 ctactacatc gccgagatca tgttcaacga atttcgtgaa aagcgtttcg cgccgccgcc
      421 gctcctcaag cgcatggtgc tggccggact ctacgggcgc aagtcggggc gcggcttcta
      481 cgactacgcc gacccgaaga atcccgtgcc gatgaacctg ttataaaaac aacgatgaag
      541 gaggaacgcg gaacgatgaa gtaaaagcaa cttgtctttc attcatcgtt cctcgtttct
      601 ccttcatcat ttcgattaaa ggggggacta tgagtaagga acaacagttg gtgctggctt
      661 cggctgccgt tggtgcggct tccgatttcg ggagcggttc gatcttcttc gtcgggacgg
      721 cgaccgtgat cctgcgttac gcgggtttca ccatcctcac cgacccgaac tttctgcacg
      781 cgggcgacca cgtgcatctc ggttacggcc tgacctcgga acgcctgacc aacccggcca
      841 tcgaactcga agagttgccg cccatagatt tctgcgtgct ctcgcattat cacggcgacc
      901 acttcgaccg catcgtggag gagcgtctgc aaaaagattt gcccatcatc accaccgagc
      961 acgcggcggg cgaactcaaa agcaaaggct tcaccgcgcc gctcgcgctc gatacctggg
     1021 acgccgtgac ggttgagcgc ggcgatgcga gcgtgcgcat cacttcgatg ccgggcaagc
     1081 acggcccgac ggctgtggac tttttcctcc cgcccgtcat gggcagcatg ctcgaattcc
     1141 gcaacgccgc cgggcagcgc acgacgctcc ggctctacat caccggcgac acgctcgtcc
     1201 acgacgacct cgaagagata ccgaagcgct atcccgaaat tgatctcgcg ctcttccatc
     1261 tcggcggcac gcgcatcatg ggaatacttc tgacgatgga cgccgaacag ggagtcgaag
     1321 cgattcgcat catcaacccg cgcgaagtga tcccgattca ctacaacgac tacggagttt
     1381 tccagtcgcc gctcgaagac ttcaaaaaag cggtggaggc cgccgggtac gcagaccgtg
     1441 taaaatatct gagccacggc gagacttacg attttgaagt gcctgccagc cgttacgctt
     1501 caacttagag aagtcccgtc agggacgatg actgactatt ttatgaaacg aaattcatcg
     1561 tacgcgctac gcttgttgct gttcatcgcc tgtctcggcc tcggctcgat tgagacgacg
     1621 caggcgcaac ggcagcaagc gtcggcgcgt tatgtctgcc ccatgcacgc ggaggtgacc
     1681 tcacgcaagc ccggtagttg tcccaagtgc atgatgaaac tggtcgcacg caagcgcgac
     1741 ggcgcgcagg cgacaactcc ggcggcctcg tcgcaaagtc agtccaccgt cgccgctgcg
     1801 actgccgagc gcgtgcgcgc gttggagcaa ctcgctccga gttacgagtt cacctgttcg
     1861 atgcaccctg agatacatca ggcgcaggaa ggcgtgtgcc cgaaatgcgg gatgccgctc
     1921 acgaccgtga agccctccgt gcgcggcgcg tatcgcctcg atgtcgtgcc gacgccgcgt
     1981 gcgccgaagc cgggcgaagc gttgcgcctg aacttcgtcg tctcgcaccc ggagacgggc
     2041 gcgcgcgtgc gcgactacgt gttgaatcac gagcggctgt ttcacctctt catcgtcagc
     2101 gcggacctga acgagtatca aca
//
LOCUS       DASWNS010000292         6671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_451874, whole genome shotgun sequence.
ACCESSION   DASWNS010000292 DASWNS010000000
VERSION     DASWNS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451874"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..697
                     /locus_tag="VGB76_10280"
     CDS             191..697
                     /locus_tag="VGB76_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0099324.1"
                     /translation="MVGNEGMFGVCVFLGTISTPLLAQVQLSGDGLRMRADALKREFN
                     KGGSLHDLLLRYTQAFVTQIALTAACNRAHQIGGRLAKWLLMCQDRSQSKDLGLTHEF
                     IAQMLGMRRAGVTEAANLLKDAGMINYHRGHVTIIDRERLEAQSCECYPLLKREFSRL
                     LGSNGHTV"
     gene            complement(785..1936)
                     /locus_tag="VGB76_10285"
     CDS             complement(785..1936)
                     /locus_tag="VGB76_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099325.1"
                     /translation="MKKILLSVCLILCGLVAPAGAQFNKSESARKAGERLARGDRAGA
                     IAVLDKAIEQRKDLQETYGMRARLRAQTGDLDGAIADFGEAIKLTPHDVALYEQRAMY
                     RLFKRDSAGALQDYDAAIANGLKSEKVYVGRASVKRDTGDVEGAILDYQSAIAVNPML
                     ASAYVGLAHVLEQKGETDAAITFLQDFLDRYEGKRGGKLPSIKGDTQQAVSSMSIKRE
                     GQEKDGGQVYLQGMGTTTVFKGGTPEEMQREMARQEQLMNLAGAYGNLGGMYARKNDL
                     ERALENFAKGLQINPGLPYIYKQRSDVRLKRGDLKGTIEDLNVVVNSPMSLPDRHLDK
                     GLLLVLQGRDDEAEKEFALHLQAFPQATRESLDTKIEAAKKLRAPQAQP"
     gene            complement(2218..4125)
                     /locus_tag="VGB76_10290"
     CDS             complement(2218..4125)
                     /locus_tag="VGB76_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0099326.1"
                     /translation="MMRIEPRSFGRTPSRALVLLLAFAAVFFFVPCETKAAAPGWSAR
                     FDGDVRFYQLTELGVVVLGTERSLYAVDGERGEILWRRKNVRLDETDIAPVPGTDLLL
                     LSLENDGKTRLEATDILTGDTIWRSDKVRGSVMQMAVDTEAQQLAVVLVRGARGKSGD
                     SLKKHPVTHVFNLASGDELWKHETGEVQLMPTSWGEDGADVETSFTLDNYHPPLFADG
                     RLYLFYEGVTSFDAATGESREREKFRVNEDGLALTEADPIFDERFIYTSGRGRLRAVS
                     RENFEVAWEAKDVGLAPEIILAGEGSTLYVRTGGQFTRLKDGETVERGPYGVSAIDTT
                     TGKVRWRYKGADKGITNLALPDRSTILIADRDDLIWLDTATGKRRAKISHRIERAAFV
                     VLNEAGAAVVGGRNEVAAFDAQSGTELWRTRHTPPGRGILRTVGAIAARAASLYFRYG
                     GTASTIFGGVRTASRAASALRWTGLSARNTLPSLTSLAAGAARQQLTRSFAPYGLAAR
                     AREAASYRLPRASVSSLPRPGLPAARDVEERLLDRLDPANQLARLSRFLWRRERLAAL
                     RGQWMYYYTDLKRASGNGLAGVNLNNGRTERELRLERPDARFTTDEEAGLLYTSQGNR
                     LNAYTLGGASR"
     gene            4488..>6671
                     /locus_tag="VGB76_10295"
     CDS             4488..>6671
                     /locus_tag="VGB76_10295"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY0099327.1"
                     /translation="MRNQLATSETLDTKLLLKTLMAFKKGDFSQRLPTEWTGEAGKIA
                     DTLNDIMEMNDQTVKELERVSRVVGRDGKITQRATLPGAHGSWTDVVDSVNMLIDDMA
                     RPTNEITRVIGAVANGDLSQRMALDVEGRSLKGEFLRSVKIVNSMVDQLGSFASEVTR
                     VAREVGTEGKLGGEAKVKGVAGTWKDLTDSVNSMAGNLTAQVRNIADVTTAVANGDLS
                     KKITVDVKGEILELKNTINTMVDQLNSFASEVTRVAREVGTEGKLGGQADVRGVAGTW
                     KDLTDNVNSMASNLTGQVRNIAEVTTAVANGDLSKKITVDVKGEILELKITINTMVDQ
                     LNSFASEVTRVAREVGSEGKLGGQADVKGVAGTWKDLTDNVNSMASNLTGQVRNIAEV
                     TTAVANGDLSKKITVDVRGEILELKDTINTMVDQLNGFASEVTRVAREVGSEGKLGGQ
                     ADVKGVAGTWKDLTDSVNSMAGNLTGQVRNIAEVTTAVANGDLSKKITVDVRGEILEL
                     KDTINTMVDQLNAFASEVTRVAREVGSEGKLGGQADVRGVAGTWKDLTDSVNSMAGNL
                     TGQVRNIAEVTTAVANGDLSKKITVDVKGEILELKNTINTMVDQLGSFASEVTRVARE
                     VGTEGKLGGQAQVTGVAGTWKDLTDNVNSMAGNLTAQVRNIADVTTAVANGDLSKKIT
                     VAVRGEILELKDTINTMVDQLNSFASEVTRVAREVGTEGKLGGQAD"
BASE COUNT         1354 a         1916 c         2160 g         1241 t
ORIGIN      
        1 ggtgcaggaa tatcttgagc atcatcggca acaatctaat caggacagcg acgcattcat
       61 cttggaatga ggcggacttt cagtcctgcg gaaatctatg cactttcagt gcatagtggt
      121 ttagttcccc agtcgcggca ccgtctctct gctgaacagt ttcgaggacg gcgggagcgt
      181 cgaagttggg atggtcggca atgagggaat gttcggcgtc tgcgtctttc tgggcactat
      241 cagcacgccg cttctggcac aggtgcaact gtcgggcgac gggctgcgga tgagagccga
      301 cgcgctcaag cgagagttta ataagggcgg ctcgctgcac gacctgctgc tccggtacac
      361 gcaagccttc gtcacgcaga tcgccctgac cgccgcctgt aaccgcgcgc accaaatcgg
      421 cgggcgtctg gcgaagtggc tcctgatgtg tcaggacagg tcgcagtcga aagacttggg
      481 cttgacgcac gagttcatcg cgcagatgtt agggatgcgc cgcgccggtg tcaccgaggc
      541 tgcgaatcta ctcaaagacg cggggatgat taactatcac cggggacacg tcaccatcat
      601 cgaccgcgag aggctggaag cgcagtcgtg cgaatgttac ccgctgctga agcgggagtt
      661 ctcacgcttg ctgggtagca acggtcatac agtgtgaaaa ggaaagcccg cacggcattg
      721 atatgcctga tgcgggcttt caccttcgta cttcccactt caaatcccaa agggcggcga
      781 gattttacgg ctgcgcctgc ggcgcgcgga gtttcttcgc cgcctcgatt ttcgtgtcca
      841 gagactctct ggtggcttgc gggaatgctt gcaggtgcag cgcaaactcc ttttccgcct
      901 cgtcgtccct gccttgcagg acgagcagca gacctttatc tagatgacga tccggcaggc
      961 tcatcggcga attgacgacg acgttcaaat cctcaatcgt gcccttcaag tcgccgcgct
     1021 tgagccgcac gtcgctgcgc tgcttgtata tgtagggaag ccccggattg atctgcaagc
     1081 ccttggcgaa gttttccagc gcgcgttcga ggtcgttttt cctcgcgtac atgccgccga
     1141 gattgccgta tgcgcccgcg aggttcatga gttgttcctg ccgcgccatc tccctctgca
     1201 tctcttcggg cgtgccgcct ttgaaaaccg tcgtcgtccc catgccctgt agataaacct
     1261 gcccgccgtc tttctcctgt ccctcgcgtt tgatcgacat gctgctgacc gcctgctgcg
     1321 tgtcgccctt gatgctcggc aactttccgc cgcgcttgcc ttcgtagcgg tcgaggaagt
     1381 cttgcagaaa agttatggcc gcatccgtct cgcctttttg ttcgagcacg tgtgccagcc
     1441 cgacgtaggc ggaggcgagc atcgggttga cggcgatggc ggattgataa tccagaatcg
     1501 cgccttccac atcccccgtg tcgcgtttga ccgacgcgcg cccgacgtaa accttctcgc
     1561 ttttcaggcc gttggcaatc gccgcgtcgt aatcctgtag cgcaccggcg gagtcgcgtt
     1621 tgaagagacg gtacatggcg cgctgttcgt agagcgcaac gtcgtgcggg gtgagcttga
     1681 tggcttcgcc gaagtctgca atcgcgccgt cgagatcgcc cgtctgcgcg cgcaggcgag
     1741 cgcgcatgcc gtaggtttcc tgcaaatctt tgcgttgttc aatcgccttg tcgagcacgg
     1801 caatcgcgcc cgcacggtcg ccccgcgcca gccgctcgcc cgccttgcgc gccgattcgc
     1861 tcttgttgaa ctgcgcaccg gctggcgcga ccagcccgca gaggatgaga cacacggaaa
     1921 ggagaatctt tttcatcagt cgctccaaga ctaaagactc gacgcgtcgc ggggggaaac
     1981 ggcagcggcg gcaattggcg gcaattgtaa cttgagatag ctgctatcta acagcagtga
     2041 cgagtgccaa aaacagtgac aagtgacggg tgacaagtta aaaagacgta aatcgtgaat
     2101 cgtaaataga cgaaggccgt ttttctcgat tcacgattca cgattcacga tttatgatcc
     2161 acttgtcttg tcgcttgctc gtcacttgtc acctgtcact cgtcactgag tttgagccta
     2221 acgcgacgcg ccgccgagcg tgtaggcgtt gaggcggttg ccctgcgaag tgtagagcag
     2281 cccggcctct tcgtcggtgg tgaagcgcgc gtcggggcgt tcgaggcgga gttcccgttc
     2341 ggtgcgcccg ttgttgagat tgacgcccgc cagcccgttg ccgctcgcac gcttcaagtc
     2401 cgtgtagtag tacatccatt gaccgcgcag tgcggcgagc cgttcgcgtc gccagaggaa
     2461 acgcgagagg cgcgcgagtt gattcgcggg gtcgaggcgg tcgaggagcc gttcttccac
     2521 gtcgcgcgcg gcgggcaggc cagggcgcgg cagactcgaa acgctcgcgc gaggcaacct
     2581 gtaactggct gcctcgcgtg cgcgcgcggc gagtccgtaa ggcgcgaaac tgcgcgtgag
     2641 ttgttgacgc gccgcgcctg cggcgagcga cgtgagactc ggcagcgtgt tgcgcgcgga
     2701 gagtccggtc cagcgcagcg cgctcgcggc gcgcgaggcc gtgcgcacgc cgccgaagat
     2761 ggtggaggcc gtgccgccgt agcggaaata gagcgaggcg gcgcgcgcgg caattgcgcc
     2821 gacggtgcgc aggatgccgc gacccggcgg cgtgtggcgc gtgcgccata actccgtgcc
     2881 gctctgcgca tcgaacgcgg cgacctcgtt gcgaccgccg acgaccgccg cgcccgcttc
     2941 gttcaagacg acgaaggcgg cgcgttcgat gcggtgcgag atttttgcgc gacgcttgcc
     3001 cgtcgcagtg tcgagccaga tgaggtcgtc gcggtctgcg atgagaatgg tggagcggtc
     3061 gggcagcgcg aggttcgtga tgcccttgtc cgcgcccttg taacgccagc gcaccttgcc
     3121 cgtggtcgtg tcaatcgcgc tcacgccgta ggggccgcgc tccaccgtct cgccgtcttt
     3181 aaggcgcgtg aactgcccgc ccgtgcgcac gtagagcgtc gagccttcgc ccgcgagaat
     3241 gatctccggc gcaagtccta cgtctttcgc ttcccacgcg acttcgaaat tctcgcgcga
     3301 gacggcgcgc agacgcccgc gcccggaagt gtagatgaag cgttcgtcga agatggggtc
     3361 ggcttcggtg agcgcgaggc cgtcctcgtt gacgcgaaac ttttcgcgct cgcggctctc
     3421 gcccgtcgcc gcgtcgaagg aggtcacacc ctcgtagaag agatagagcc gcccgtcggc
     3481 aaagagcggc gggtgatagt tgtcgagcgt gaaactcgtc tccacgtccg cgccgtcttc
     3541 gccccaactc gtcggcatca actgcacttc gcccgtctca tgcttccata gctcgtcgcc
     3601 gctcgccagg ttgaagacgt gcgtgacggg atgcttcttg agactatcgc ctgatttgcc
     3661 gcgcgccccg cgcacgagga cgacggcgag ttgctgcgct tcggtgtcca cggccatctg
     3721 catcacgctg ccgcgcacct tgtcgctgcg ccagatggtg tcgcccgtga gaatgtccgt
     3781 ggcttcgagg cgcgtcttgc cgtcattttc cagactcagg agcaggaggt ctgtgccggg
     3841 cacgggcgcg atgtcggttt cgtcgagccg gacgtttttg cgtcgccaca aaatctctcc
     3901 gcgttcgccg tcaaccgcgt agagcgaacg ctccgtgccg aggacgacca cgcccaactc
     3961 cgtcaattgg tagaaacgca cgtcgccgtc gaagcgcgcc gaccaacccg gcgcggcggc
     4021 cttcgtctcg cacgggacga agaagaagac ggcggcgaag gcgagcagga ggacgagcgc
     4081 gcgcgaaggc gtgcgcccga aggaacgagg ttcaattcgc atcataaaaa aactccccga
     4141 gtcgtctaac ttgagagacg cttaatgtta gtctataacc gcgccgcgcg aatacaaaac
     4201 ggagggttcc ggcgagccgc gtgcgcgcgg gatttacata ttttcgccgg aaaggtataa
     4261 attaagcggc gaacgacttg ataaccagcg caacccaatc actcccccga tactctccac
     4321 ccattccggc agtggccgtg ctgtctcctc cggcggcaag agcgcggcgc gccaccatgc
     4381 gatcatcaac agctaagatg cgccgaagtg aaatgcggtc gggagctttt tagccgcgag
     4441 ccgaaatctc tcgacgagaa tactcaataa ttttttggaa ggacgcgatg cgaaaccaac
     4501 tggcaacttc agaaacactc gacaccaagc tgctcttgaa aaccctgatg gccttcaaga
     4561 agggcgactt ctcccagcgt ctcccgaccg aatggacggg cgaggccgga aagatcgccg
     4621 acaccttgaa cgacatcatg gaaatgaacg accagaccgt caaggaactc gaacgcgtga
     4681 gccgcgtcgt cgggcgcgac ggcaagatca cgcaacgcgc cacgctgccc ggcgcacatg
     4741 gttcgtggac ggacgtagtt gactcggtca acatgctcat cgacgacatg gcgcgcccca
     4801 cgaacgagat cacgcgcgtc atcggcgcgg tcgccaacgg cgacctctcg cagcgcatgg
     4861 cgctcgacgt ggaaggccgc tcgctcaaag gcgagttcct ccgctcggtc aagatcgtca
     4921 actcgatggt ggatcaactt ggctcgttcg cctcggaagt gacgcgcgtg gcgcgcgaag
     4981 tgggtacgga aggcaagttg ggcggcgaag caaaagtaaa aggcgtcgcc ggaacatgga
     5041 aagacctcac cgacagcgtt aattcgatgg ccgggaacct caccgcgcag gtgcgcaaca
     5101 tcgcggacgt gacgacggca gtagcaaacg gcgacctctc gaagaagatc acggtggatg
     5161 tgaagggcga aatccttgag ttgaagaaca cgatcaacac gatggtggat caactcaatt
     5221 cgtttgcgtc ggaagtgacg cgcgtggcgc gcgaggtggg tacggaaggg aaactcggcg
     5281 ggcaggcgga cgtgcgcggc gtggcgggta cttggaaaga cctcaccgac aatgtgaact
     5341 cgatggcgag caacctcacc ggccaagtgc gaaacatcgc cgaagtgacg accgccgtgg
     5401 caaacggcga cctctcgaag aaaattactg tcgatgttaa aggcgagatt ctggaactca
     5461 agatcaccat caacacgatg gttgatcagt tgaactcgtt cgcttcggaa gtgacgcgcg
     5521 tggcgcgtga agtgggtagc gagggcaaac tcggcgggca ggccgacgtg aagggcgtgg
     5581 ccggaacgtg gaaggatttg acggataacg tgaactcgat ggcgagcaac ctcaccggcc
     5641 aagtgcgcaa catcgcagag gtgacgaccg ccgtagcaaa cggcgacttg tcgaagaaga
     5701 tcacggtaga cgtgcggggc gagattctgg agttgaaaga cacgatcaac acgatggtcg
     5761 atcaactcaa cggctttgcc tcggaggtga cgcgcgtggc gcgcgaggtg ggcagcgagg
     5821 gcaagctcgg cgggcaggcc gacgtgaagg gcgtggccgg aacgtggaag gatttgacgg
     5881 atagcgttaa ttcgatggcc gggaatctga cgggtcaggt gcgcaacatc gcagaggtga
     5941 cgacggcagt agcgaatggc gacctgtcga agaagatcac ggtggacgtg cgcggggaaa
     6001 ttctggagtt gaaagacacg atcaatacga tggtggatca actcaacgca ttcgcttcgg
     6061 aagtgacgcg cgtggcgcgc gaagtaggta gcgagggcaa actcggcggg caggccgacg
     6121 tgagaggcgt ggccggaacg tggaaagatt tgaccgacag tgtgaactcg atggccggta
     6181 atttgacggg tcaggtgcgc aacattgcag aggtgacgac ggcagtagcg aatggcgacc
     6241 tgtcaaagaa gatcacggtg gatgtgaaag gcgaaatcct tgagttgaag aacacgatca
     6301 atacgatggt ggatcaactc ggctcgttcg cctcggaagt gacgcgcgtg gcgcgtgaag
     6361 tcggtacgga agggaaactc ggcggacagg cgcaggtaac gggcgtggcg ggcacttgga
     6421 aggacttgac cgacaacgtg aactcgatgg ccgggaacct caccgcgcag gtgcgcaaca
     6481 tcgcggacgt gacgacggca gtagcaaatg gcgacctgtc gaagaagatc acggtagctg
     6541 tgcgcgggga aattctggag ttgaaagaca cgatcaatac gatggtggat caactcaact
     6601 cgtttgcgtc ggaagtgacg cgcgtggcgc gcgaggtggg tacggaagga aagttgggcg
     6661 ggcaagcgga c
//
LOCUS       DASWNS010000293        28636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_220559, whole genome shotgun sequence.
ACCESSION   DASWNS010000293 DASWNS010000000
VERSION     DASWNS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 28636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28636
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220559"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VGB76_10300"
     CDS             <1..618
                     /locus_tag="VGB76_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099328.1"
                     /translation="IVVLNPVTATVERVISLQYNPDTLSRSLQIKGLGAGGGDRSEAL
                     RLTGPPVETIKLDAEIDATDQLEFPEQNPNAVQVGIHPQLAALETLVYPTTGQIQSNI
                     NLAQSGTLEITPMESPLTLFVWSKSRILPVRLTDFSVTEEAFDPLLNPIRAKVSIGMR
                     VLSSDDLKPAHRGHSIFMAYHQQKQRLAALYDGGALGGLGITKIP"
     gene            657..965
                     /locus_tag="VGB76_10305"
     CDS             657..965
                     /locus_tag="VGB76_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099329.1"
                     /translation="MFPFTSRYHTTRTAQLEGADGEPVVYLRRRFVPPPERFELLTEH
                     GVTEGERLDNITAQYLGDPEQFWRICDANGAMRPDELTEAIGRRLRITLPEGFQTPHA
                     "
     gene            958..2088
                     /locus_tag="VGB76_10310"
     CDS             958..2088
                     /locus_tag="VGB76_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099330.1"
                     /translation="MLKGINLTLMIGPGVPVPVPAEVLDALTSVTVQTSTAQQRSGFS
                     LTFSLSNNSPLQTIFLLTAGSLPPIMRVVIVVTINGTPEVLMDGVITRHEITPGNDPG
                     HSTLTLSGEDLLALMDLIDFTGIPYPAMPYEARVALCIAKYAFFGMIPAVLPSVLIDI
                     PIPVERIPRHQGTDLAYINYLAEFVGYVFYLTPGPEPLMSTAYWGPEIKVGTPQPALS
                     INMDAHSNVETLNFNFKPDQATLPIIFIQNQLTKVPIPIPIPPITPLNPPLGVVQPFP
                     QKIEFISETAKYNPIQGALVGLAKAAKTADTVEATGSLDVMRYGRLLKARKLVGVRGA
                     GLAFDGLYYVKSVTHEIKRGEYKQSFELSRNGLISITPKVPV"
     gene            2085..2597
                     /locus_tag="VGB76_10315"
     CDS             2085..2597
                     /locus_tag="VGB76_10315"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEY0099331.1"
                     /translation="MSTIKKYYGKYRGTVINNLDPMQMGRILVIVPDVSVIPSSWAMP
                     CVPVAGIQTGMYAVPIIGSGVWVEFEQGDPDYPIWSGCFWGSAAEIPVLARLAPPVIP
                     SITLQTPLQNGLTISDVPGPTGGIMIKSTTGAAIIVNDTGIYIQNGKGASIVMVGPSV
                     TINAGAMTII"
     gene            2673..2996
                     /locus_tag="VGB76_10320"
     CDS             2673..2996
                     /locus_tag="VGB76_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099332.1"
                     /translation="MPGFLLHVGATVMCAHGGQAQPAVPNPRVLVSGQPTVTIASPYI
                     VAGCAMPPPSAGNGPCVTAQFVTAATRVFSNGQPLLLIDSQAVCVPTGTPLIPSVTQV
                     RVTGM"
     gene            3012..3377
                     /locus_tag="VGB76_10325"
     CDS             3012..3377
                     /locus_tag="VGB76_10325"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEY0099333.1"
                     /translation="MNVDFPYHFDGSGRTARVEGDAHIRDLIEQVIFTMPGERVNRPT
                     FGCGLAQLVFAPNSFELAAASQLLVQSSLQQWLGDLIQVEDVQVEAEDSTLRVVVQYV
                     VRRTQGREVVRFARGGTSA"
     gene            3374..5875
                     /locus_tag="VGB76_10330"
     CDS             3374..5875
                     /locus_tag="VGB76_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEY0099334.1"
                     /translation="MKYFCCEERRRNAVAAHPTLNGIEFLEVSDDPANPPEQRQRTLH
                     VHFIKDLAPDALTVQNIRIEGGERIRQIKATKITVAPAGVANVVSVEVERAGDFSTYT
                     LRLVKSSDDADTPDGFDPVLSAVDFSFKVACPSDFDCEDASACPPEHLPQPLINYLAK
                     DYASFRQLMLDRMSVLMPQWTTRNPADLGVALVEMLAYVGDHLSYQQDAVGTEAYLGT
                     ARRRTSVRRHARLVDYRMHDGANARAWVQVQVEGGNVELDKGAQIFSRLPHLPQRIQP
                     ASLDYDVATAARPVVFETMQKATLHFAHNTIFFHTWSDERCCLPRGATRATLRNEGDT
                     LAALAVGDVLIFVETRNPQNGNAAEADPAHRHAVRLTKIKPATDPLYTEADGVEPLRV
                     LDIEWAADDALPFPLCLWEVETGGEANEKTPASVALGNIVLADHGLSIAGETFDAVPP
                     HDPALNRAASHGSDSCAERKTRPSPPRFRPQLAQRPLAYVAPFDPAQPSISASAAMNW
                     PVSEFVPAISLTGALNAQTATWLPQGDLLASEAGATEFVVEVETDGTAYVRFGDDRFG
                     SRPPTGTIFTADYRVGGGVAGNVGAGALAHIVTNHAGVVGVTNPLPARGGVAPESIEQ
                     VRQNAPYAFRTQQRAVTAKDYAEVAERHAGVQRAAATIRWTGSWRTVFLTVDRLGGAD
                     VDADFEQEMRAHLERYRMAGHDVEIDAPRYVSLEIEMTVCIKPGYLKSDVRRALLELF
                     NRHTRPDGRRGVFHPDNYSFGQTVFLSPLYAAAQSVDGVDSVEIKRFARRGETGDAAL
                     EAGKLELSRLEIARLDNDPNFPERGVFQLTLKGGR"
     gene            5872..8601
                     /locus_tag="VGB76_10335"
     CDS             5872..8601
                     /locus_tag="VGB76_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEY0099335.1"
                     /translation="MSTALDPTLHHLDDCGCCEGVAARTPVEIFNRPGLSAIAYRAGR
                     HAQFKQTLLARLSNKDRPALARLTTRDADDFSIALLDAWATVADVLTFYQERIANESY
                     LRTATERLSLLELARLIGYELRPGVAASAYLAFTIEEVPGATREMAAALGVPRETTID
                     PGVKAQSVPGPGERAQMFETVERIEARAGWNEIRPRLTERQVVKLNAGALYFEGLATG
                     LKKGDGLLITEDDYHESMFREVSEVTLEPELTRTKVHLKPTELQTFANIKIQLGALTT
                     AVTSAVTQKFIGQTVRARDLRASALSQKFQIADLFDNILAVRQPPPAVLALRTRASIF
                     GHNAPRWESLPAVQRIGEYVAVDGGVDPSNVEFKGGVYRNRANSWAEESLYDYHRLPG
                     EPANKHDIYLDAAYPSIVKGSWIVLKQDDDAYAYQVKATSEVSRADYTLSGKSTRLRL
                     DRFTGLGNFRIRRTTVHAQSEELALARLPIETPVAGKELDLETWVDGLSEGQSIIVCG
                     ELDDASGVNACEHALIKDVEHVLETEGFTRLTLVAPLENSYVRATVKINANVALATHG
                     ETVTEPLGSGDAGRVFQRFTLRQPPLTYVSAKTPTGSATTLSVRVNGLLWHEVPSFYG
                     HGADERIYITRTDDDGNTTVQFGDGRTGARLPTGQDNVTATYRKGTGLAGLVRANQLS
                     QLMTRPLGVRGVINPSAASGAADREERDEARRNAPLTVMTLERVVSLADYEDFARAFA
                     GIGKAQATWTWNGEQRGLFLTLAGANGAEIESPLDKNLLAALRASGDPAVPLEVASYQ
                     PRLFRLKAKVRVHPDYLPENVLREVGRNLRARFSFDARAFGQPVALSEVIGCMQRVAG
                     VVAVDVDELYRTGETATLNTRLPAAAPRAGSRQAAPAELLTLDPGALALEVMQ"
     gene            8598..10670
                     /locus_tag="VGB76_10340"
     CDS             8598..10670
                     /locus_tag="VGB76_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099336.1"
                     /translation="MSFDKEKLYALLPAVYRLRDDEQGKPLKALLSVIAEQVAVLEED
                     LEQLYDDQFIETCAEWVVPYIGDLVGARGLQTWPEATFSHRAQVANTLAYRRRKGTAA
                     MLEQLARDVTGWPSNVVEFFELLSTTQYMNHIRLRHPVEVDLRGWKALHPATVDLRDW
                     EALELLDTPFERLAHTADVRHIETRRGLYNIPNVAIFLWRLAGYLLTDAPAHRLDARR
                     FRFNPLGFDTPLYNRTVTEKEITHLAETVNVPAPLRRRLLERDLELHYGANQSFHLKV
                     DDVPIDADAIRICNLSDDGAGWLDKANDKHVVDPVLGRIMLPEGVYADPAKVRVSFRY
                     GFSAEMGGGQYGRASTFTNDLEPLLKVPTHHAMLQDALDNLDGNGVVEIENNEYYFEQ
                     FFINAAADGKVELRAADERRPVIAATGDFWITGGDGSEVTLNGLVLAGGTVRVPLEKP
                     GGQPNELRVLRISHCTLLPGATPAIGNVPAQSPTVRIRIDAPNVTVEIDRSIVGMIRA
                     VEGARVRITDSILDAGAESSPAYADPALAAGASLHISNSTVVGRVHTELIEASNTIFM
                     SGADASDPSLLPLRVERLQEGCVRFSYVPPGSRVPRQYHCQPLQGDTAEQRARVRPLF
                     ESLTYGDAAYCQLSPRCAAEIRQGADDEAAMGAFHHLYEPLREANLRARLDEYLRFGL
                     EAGIIYAS"
     gene            10823..15232
                     /locus_tag="VGB76_10345"
     CDS             10823..15232
                     /locus_tag="VGB76_10345"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEY0099337.1"
                     /translation="MKGDFSRRTFDRAKRYSGVLMQQGRVQLDADWNEQLAIHQHRTE
                     TETKDVIGLCGVPKNSDGFRLQPVTPANMSATTDLLIFPGRIYVNGLLCEADAEPVAI
                     TGFTPAGAIVAHMSADGRAFESGQWVEITGDDEAKTLRLITAVDAAQNSLSFDKAPPN
                     YTPSQHPHLRRLTTYLTQPDLPAPAQAASYGEEEAATLELGEEYYLAYLHVREQHITA
                     LEDPSIREKALGGPDTTTRIKNVWQVELLPVAIPDDGALSCETEFPEWTALNAPSTGT
                     LNARTSPADIPTDPCQLPPGAGYRRLENQLYRVEIQKGGERDGATFKWSRENGSIQTT
                     ITEINGNIVTVADLGRDEVLGFAGGQWVELVDDESELQGEPRPLAFINSIDLSSREIT
                     LDTDATSLAGKKNLRLRRWDQSGGKAGADGLTMSDDWLSLEGGVEVRFSDGTYRAGDY
                     WLIPARTATADIEWPSDSASGDAPLPQPPHGVRQYYCRLALFRVDDAILWLVEDCRDF
                     FPALTDICAEDVCFDNSNCDLPNAETVQDAIEALCGANDLRFHNKHLHGWGIVCGLQV
                     NCGPNEPESPRRYVTVRSGYAIDCEGNDIIHRNDEPVNILEMIQAYNLQHPNAPILQG
                     DGEVCLVLNTDGQNPYALEKYDPAWKSWKSALEGTLFKDFFQDCLGNLLLFFKEELSP
                     KPGEANLLVSPTQKLITSLLNLVVQLFNPTNGRYVYLSGEQNVEIDSTEHTILKNFYE
                     KLRGLLQSHTFCAMFDQATPFPVYPYSMYNGSDSVPTYIPTIFGKGFHRRLRVHPGGK
                     LAYTVGAANKIHVYDLERNEMVEEFEYPIGNAVVRDVAFSPDGNQLYAIATVNNKESS
                     FSVFDVQIGSGSVKHVWRKKGILCDFLLVTLATAEDGKVYGVGRGRGLYILHMEKSEP
                     DATLFGQSFNASGHLVVVRRGAQQFAFATAQEPGNSLDQYNHVRRFNLLNANENPMFL
                     QLQIQGQSVSGQDDIAVASDDKNAKLYVVTNPPSFSDNNHLIIFNNAFSASPPTQQFI
                     NLEEKTGMRLAYNYPTQNLMITYSDSYQVRLVGQNNQLVAGHRQAVQIAPTSIATTPD
                     QKRVLVLNYGSNTITSLRADLVKANSFPPLEGVSGLGPYRDQILLAFVDLFGGLLQYL
                     KDCLCDHFLVDCPECDEADKIYLACISIRQEEVYQVCNFSRRKYVKSFPTVEYWLSLV
                     PVIPLVGKFVEQFCCTILPEFFVGKYKTSAAANAAAPSQNKLSSEAMYKISSVLQQTS
                     FGNMFGDALKKFGWIGGQLIGDWFECVFKRQNLPSGEIDRGGEGSPGTTGMAQLSENE
                     MERDPCNWCGNLVRYICPPRTQGEGRDATGASVETDFRRDATFTAASAETVLMERTAS
                     GSQTEGVSASEDVLSLRNEMAALREEFARTQGSYAGALAARDATIASLEATTRDLQAS
                     LATLSELQEKVNSLTPTRRKPRQRTKKEDKSAQ"
     gene            15875..16666
                     /locus_tag="VGB76_10350"
     CDS             15875..16666
                     /locus_tag="VGB76_10350"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY0099338.1"
                     /translation="MKTIAIANQKGGVGKTTTAVNLAACLARLGRRTLLVDLDPQANA
                     TDHLGFENPEGELHSSYALISEKMPDMSSILRAVGPNLQIAPGHIALAEIDIKLFSSL
                     NRETRLLRALSQVENQFDFVIIDCAPSLGISTVNAFCASTHIIITIQTNWFAYEALKR
                     LMAIASDVMEESNPNLEIYALATLHRANVNVNREVLQKIQEDFGGLAISAVIPHTATL
                     AEASVAKQPIIDYAHGSKGHQAYQKLAEEIISRVERQQTEEAQVS"
     gene            16626..17705
                     /locus_tag="VGB76_10355"
     CDS             16626..17705
                     /locus_tag="VGB76_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099339.1"
                     /translation="MSSDNKPKKRKFPEIPSVRATPGETTATQNTVKRMLGRLPLERE
                     GTEVEPHPASPTNHPPTTHQPPTNQPIAPERNFSRVPNSIALSGIPSGLFRGESKKLY
                     DALYQRTRGAVVPRRAIRATQSELMGWANISLNTLKAHIRHLSSIGLLKVHYRRGEST
                     GADYEIFTLEDRPPTNHPPTTQPPTTNQNLGSPTHQNLVLGGGSQVTVESTLAASPKT
                     SFKTKEENSDDDAPARTFLAKLVEAERELTGKASTSGEKWEELAEVLISELRIAAART
                     TVSNVPAFLAEHLRRRLWKVDKARAAEMAASQPEQEGVAPALTTEEKQKCPDCAGVGF
                     WYPEGPEKGVARCSHLKLHSLLAGQ"
     gene            complement(18309..19628)
                     /locus_tag="VGB76_10360"
     CDS             complement(18309..19628)
                     /locus_tag="VGB76_10360"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0099340.1"
                     /translation="MKKSQTAFVLAIVLMATGTACAQGGRDAVKTSATPASPTSNAPC
                     APLETRTANAPEQKPAFPGQTRACAVKSGVAFDVTVLAKGLERPWAVEPLPGGDLLIT
                     EKPGRLRIVSAKGEVGAPIGGLLPVGSGGVSAASGQGGLPPITARGQGGLLDVALSPN
                     FSRDRTIFWSFSEQREGGSGTSVARGRLTEDRRNLDQVRIIFRAMPTYNNGLHFGSRL
                     AFGQDGMLYITLGDRFDKTITRPQAQHLNSHLGKIIRINPDGSVPADNPFAKQSGALP
                     EIWTLGHRNLQSAAFDDRGRLWTVEHGAKGGDELNLIERGKNYGWPVATYGEEYSGEP
                     ITGAVTAREGYEQPVYYWDPVIAPSGAQFYTGDAFPQWRGSLFIGGLVSTRLVRLVIK
                     ENRVVGEEHLLTERGQRIRDVRQGHDGTLYVVTDEANGELWKITPRR"
     gene            complement(19715..21103)
                     /locus_tag="VGB76_10365"
     CDS             complement(19715..21103)
                     /locus_tag="VGB76_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEY0099341.1"
                     /translation="MNRTRTLSLNVRFFAVALVLGVALPALAQPVDDWARVEEVVRSI
                     RLPSIPDRDYRITDFGAQAGGTDDARPAIMAAIKKASAEGGGRVVIPPGKWLSRGPVH
                     LSSRINLHLAEGATLLFSPEARHYLPVVLTRWEGTEVHSYSPLIYAFEVRDVAITGKG
                     TIDGNAASEFLTWIPRAEPDFQRLRRMGADGTPVARRVFGEGTHLRPSIIQFFRAERV
                     LLEDYTVLNSPFWVNHLVYTDHATVRRIRVDSHHANNDGVDVDSSRHVLIERCWFRTG
                     DDSVVVKSGRDRDGREIGRPSEHVVVRHNDMGGEDGIALGSEMSGGIRHVYFTDNILR
                     KGASAIRFKANLDRGGTVEHIRVRNFTVEAFDNLFWFQLDYPGQLGGNFPSTYRDIVF
                     ENFTVERAGTVFNGDAPAAAPLRDVTLRNVNIKQADRTFVLKNVEGLKLENVRIGGQR
                     VDGRLEWRQAAP"
     gene            complement(21125..22183)
                     /locus_tag="VGB76_10370"
     CDS             complement(21125..22183)
                     /locus_tag="VGB76_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0099342.1"
                     /translation="MRKDVYLLLVSFVLTLAVLTASGQGQETKPAAQGRAATPALDPE
                     LTNYQYPHPVSFYEFDTQGQRLRMAYMEIAPARPNGSTVVLLHGKNFSGAYWSPTIKA
                     LSDAGYRVVAPDQIGFGKSSKPASYQFSFQALAANTRSLLDSLRVDKFALVGHSMGGM
                     LATRMALMYPGRVERLALVNPIGLEDWKLSAPYRTVEENFAQELRATPESIKEYQRVN
                     YFGGEWKPEYDALTEILAGWTRHPDYRRVAWNAALTSDMIFTQPVLYEFERLKTPTLL
                     IIGQRDRTAIGKDRVPKEVAATMGDYPALGRAAARRIPRASLVEIGEAGHIPQVDSFA
                     KYIRALLDFIAGANSPQP"
     gene            complement(22314..25235)
                     /locus_tag="VGB76_10375"
     CDS             complement(22314..25235)
                     /locus_tag="VGB76_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0099343.1"
                     /translation="MRAERFRQIDALLDVAFEKDAGERTAFLEAACAGDDELRAEIES
                     LLAAHGRAENFIEQPALEVVARAAARGGDTAEAGRRVGSYRILSLLGAGGMGEVYLAE
                     DERLKRRVALKLLPAGFTKDPERVRRFEHEARAASALNHPNIVTIHEVGQSEGTHFIA
                     TEFVEGRTLRRLIAAGELQNADALSIAAQVAEALVAAHAAGIIHRDIKPENIMLRPDG
                     YAKVLDFGIAKLTERSSSPDAPHHLAESVQTETGTVVGTVAYMSPEQALGQKVDHRTD
                     VFSLGVILYEMLTGTHPFRGLTNAATFDRILNHDPPTPNIPNSDAAPDLERVVCRALE
                     KDRERRHQTASDLCTELKRLQRALDSSPQVSDKFVADAARTRPPRSLSRRTARFALAS
                     LLIVVAATVVYFAWRRNAVAPAREAAGVAGARFNVTQLTTQPGQEHYPSLSPDGKALI
                     YAARAPDDWDIYWQRVGGSNPLNLTENSPADDTHPALSPDGEYVVFRSEREKGGIFIM
                     GATGESTRRLTDFGFYPSWSPDGREVAFSTEGFTDPNVRGLGSSMIWIVNVSTGERRQ
                     LTDAKTGDAAQPAWSPSGARIAYWGKHKGGQRDIWTVPARGGGDSEPVGVTDDAAFDW
                     NPVWSPDGKFLYFASDRGGSMNLWRVPLEEQTGRATGAPEPLNTPSSYAYQLAFSRDG
                     RRAAYVNQVSSTNIFKVGFNPYKETITGQPVAVTQGSRHASAPNLSPDGELFVYSSQG
                     EKQEDLYVVGKDGAESPRKLTDDLYKDRSPRWSPDGTRIVFYSDRSGRYEAWMIRPDG
                     SDLRQITFTEGSSIAYPFWSPDGTRIGYNRHLESCSIIEVEKPWNEQAPYRLIDHTRR
                     RLSDYWVNSWSPDGRKLAGWSSPSGKTPGLVLYNFETGDFEWITDFGYSPVWLRDNRR
                     LIFNHQHKIYLVNTETKKSHELFPPSHHRVSGYGLSTDNRMLYYGLAIREADVWLLNL
                     D"
     gene            complement(25225..25821)
                     /locus_tag="VGB76_10380"
     CDS             complement(25225..25821)
                     /locus_tag="VGB76_10380"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0099344.1"
                     /translation="MADPSTHDVTELLAAWSGGDKAALERLVPLVHAELHRLAHRYMG
                     RERAGHTLQTSALVNEAYSRLINWKDARFENRAHFFGVSAQLMRRILVDFARSRPKAR
                     EGTARRVALDEALLVVSDERDTDLVALDEALEELAKLDARKSKIVELRFFGGLSVEET
                     AQFLNISPVTVMREWAKAKAWLYRELSAGEEEGGGIAG"
     gene            25993..26706
                     /locus_tag="VGB76_10385"
     CDS             25993..26706
                     /locus_tag="VGB76_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HEY0099345.1"
                     /translation="MTRNRIAKSLLVAVLLGALVAALSPFNSVPNTSAQAKQPAPQSK
                     TKFRRTRVTAADIARLPRGKNYVVQISPGTQSRAGTYSGSTTVNSGVLNSRDEGGKSG
                     LTKAGAGTLTLAGTNNIYEFVSDTPIDFSRVVVQTSPDAAPESLEAWLRKHRPASGMR
                     GWPFRRLLIGSAEGIAEVEGWKIRDAAPGTEYKCESGGEGDDYCGCSSILDCTLMVVA
                     GKCSSSLSCGDGECYCDAR"
     gene            26738..27199
                     /locus_tag="VGB76_10390"
     CDS             26738..27199
                     /locus_tag="VGB76_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099346.1"
                     /translation="MKMNRSGARAALLGALLLYAACAGAASEHLMPASEAEAVAAVAR
                     TALARLPAEARLDEIRDGHLRRAVQTTYRAVVELADNRDSARVAALNSKFERAYASVA
                     REIARGEHRTCAANCKSFDGDPCVAKCKADGKKFCGCKLILFGCVVAECLF"
     gene            27740..28627
                     /locus_tag="VGB76_10395"
     CDS             27740..28627
                     /locus_tag="VGB76_10395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0099347.1"
                     /translation="MERKVASDYPQELLDLFHEYQHGDIDRRTFLSRAGKFAVGGLTV
                     AAIFESLTPNYAWAQQVPPDDKRIKVGYEVVQSPAGNGSIKGYLARPAQGKKLPVVLV
                     IHENRGLNPYIEDVARRLALAKFIAFAPDGLTSVGGYPGSDEKGAVAFRTVDGKKMTE
                     DFVAAAKWLKARPDSTRKLGAVGFCFGGGMVNQLAVRLGAELNAGVAFYGRQAGTADV
                     PRISAPLLFHYAGNDERINEGIAAYEAALKANKKIYTSHMYEGKQHGFHNDTTPRYDE
                     AAAKLSWTRTLEFFNKHLR"
BASE COUNT         5612 a         9159 c         8630 g         5235 t
ORIGIN      
        1 atcgtcgtgc tcaaccctgt gacggcgacg gtcgagcgcg tcatctcttt gcagtacaac
       61 cccgacacgc tctcgcgcag tctacagatc aaaggactcg gcgcgggcgg cggcgaccgc
      121 tcggaggcgt tgcgcctgac gggtccaccc gtcgaaacca tcaaactcga cgccgagata
      181 gacgccaccg accaactcga atttcccgaa cagaacccga acgccgtaca ggtggggatt
      241 cacccgcaac tggccgcgct cgaaacgctc gtctacccga ccaccggcca gattcagtcc
      301 aacatcaatc tcgcccaatc gggcacgctc gaaatcacgc cgatggagtc gccgctgacg
      361 ctcttcgtct ggagcaagag ccgcattctg cccgttcgcc tgaccgattt cagcgtcacg
      421 gaagaggcgt tcgacccgct cttgaatccg attcgcgcga aggtcagtat cggcatgcgc
      481 gtcctcagca gcgacgatct caagcccgct cacagaggcc acagcatctt catggcttac
      541 caccagcaga agcaacgtct cgccgcgctc tacgacggcg gcgcgctcgg cggcctcggc
      601 atcaccaaaa ttccctgagt ccgtgcgccc gtaaaattca ctgagaggga gagcttatgt
      661 ttccgtttac gagtcgttac cacacaaccc gaaccgcgca acttgaggga gccgacggcg
      721 agcctgtcgt ctacctgcgc cgccgcttcg tccctccgcc cgaacgcttc gaactgctca
      781 ccgagcacgg cgtcaccgaa ggcgaacgac tcgacaacat cacggcgcag tatctcggcg
      841 accccgaaca gttctggcgc atctgcgacg ccaacggggc gatgcgtccc gacgagttga
      901 ccgaagccat aggccgccgc ctgcgtatca cgctgccgga gggattccaa acgccgcatg
      961 cttaaaggca tcaacctgac attgatgatc ggacccggcg tgcccgtccc cgtgcccgca
     1021 gaggtgctcg atgcgctcac cagcgtcacc gtgcaaacgt ctacggcgca gcagcgcagc
     1081 ggctttagtc tgaccttctc gctcagcaac aattcgccgc tgcaaacgat cttcctgctc
     1141 acggcgggga gcctgccgcc catcatgcgc gtcgttatcg tcgtgaccat caacggcacg
     1201 ccggaggtac tgatggacgg cgtgatcacc aggcatgaga tcacacccgg caacgacccc
     1261 ggccattcga ccctcacgct ctccggcgaa gacctgctcg ccctgatgga cttgatcgac
     1321 ttcacgggca tcccctaccc ggccatgcca tacgaagcgc gcgtcgccct ctgcatcgcc
     1381 aagtacgcct tcttcggcat gatcccggcg gtgctgccga gcgtcctcat agacatcccg
     1441 atccccgtcg agcgcatacc acgacatcag ggaaccgacc tcgcttacat caactacctc
     1501 gccgagttcg tcggctacgt cttctacctg acgcccggac cggagcctct gatgagcacg
     1561 gcctactggg ggccggagat caaggtgggc acgccgcagc ccgcgctcag catcaacatg
     1621 gacgcacact cgaacgtcga gacgctgaac ttcaacttca agcccgatca ggcgacgctg
     1681 cccatcatct tcatccagaa tcaactgacc aaagtgccga tcccgattcc cattccgccg
     1741 atcacgccgc tcaacccgcc gctcggcgtg gtgcaaccgt tcccgcaaaa gatcgaattc
     1801 atcagcgaga cggccaagta caacccgatt cagggcgcgc tcgtgggctt ggcgaaggcg
     1861 gcgaagacgg cggacaccgt cgaggccacc ggctcgctcg acgtgatgcg ctacgggcgg
     1921 ctgttgaagg cgcgcaagct ggtcggcgtg cgcggcgcgg ggctggcctt tgacggcctc
     1981 tactatgtca agagcgtgac gcacgagatc aagcgcggcg agtacaagca gagtttcgag
     2041 ttgagccgca acgggctgat ttccattact ccgaaggttc cagtatgagc actattaaaa
     2101 aatattacgg caagtatcgc ggcacggtca tcaacaacct cgacccgatg cagatggggc
     2161 gcatcctcgt tatcgtgccg gacgtgtcgg tgatcccttc cagttgggcg atgccctgcg
     2221 tgcctgtggc gggcatccag acggggatgt acgccgtccc gataattggc tccggcgtgt
     2281 gggtcgagtt tgagcagggc gacccggatt atccgatctg gagcgggtgc ttctggggca
     2341 gcgcggcgga gattcccgtg ctcgcgcgcc tcgcgccgcc cgttattccg agcatcacct
     2401 tgcagacgcc cctgcaaaac gggctgacca tcagcgacgt gcccggacca actggcggca
     2461 tcatgatcaa gagtacgacc ggcgcggcca tcatcgtcaa cgacacgggc atttatatcc
     2521 agaacggcaa gggcgcatcc atcgtcatgg tcggaccctc cgtgacgatc aacgcgggcg
     2581 ccatgaccat catttgagac gcgactacgt acgcgccgcg cgtttccggc ggcacgcacg
     2641 cgcgcagaca tgctaacggg aggacaagac agatgccggg atttctctta cacgtcggcg
     2701 cgacggtgat gtgcgcgcac ggggggcagg cgcagcccgc tgttccgaat ccgcgcgtgc
     2761 tcgtgagcgg ccaaccgacc gtgacgatag ccagccccta catcgtcgcc gggtgcgcga
     2821 tgccgccgcc ctcagcgggt aatggtccat gcgtcacggc gcagttcgta acggcggcca
     2881 cgcgcgtctt ctcgaacggg cagccgctcc tgcttatcga cagtcaggcg gtctgcgtcc
     2941 cgaccggcac tccgctcatc ccctccgtga cgcaggtacg cgtcaccgga atgtaaggcg
     3001 aggccacttc aatgaacgtt gactttccat accacttcga tgggagcggg cgcacggcgc
     3061 gcgtcgaggg cgacgcgcac atccgcgacc tcatcgagca ggtgatcttc accatgcccg
     3121 gcgagcgggt caatcgcccg accttcggct gcggcctcgc ccaactcgtc ttcgccccca
     3181 actccttcga gttggccgcc gcctcgcagc ttctcgtaca gagttccctc cagcaatggc
     3241 tcggcgatct gattcaggtt gaagacgtgc aggtggaggc ggaagactct acgctgcgtg
     3301 tagtcgtgca gtatgtcgtg cggcgcacgc aggggcgcga ggtggtcagg ttcgcacgcg
     3361 gagggacgag cgcatgaaat acttctgctg cgaggagcgg cgtcgtaacg ccgtcgccgc
     3421 ccacccgact ttgaacggca tcgagtttct cgaagtctcc gacgacccgg ctaacccgcc
     3481 ggagcaaagg cagcgcacac tccacgtgca tttcatcaaa gacctcgcgc ccgacgctct
     3541 caccgttcag aacatacgga tcgaaggcgg cgaacgcatc cgtcagatca aagccacgaa
     3601 gatcaccgtc gcgcccgcag gcgtggcgaa cgtcgtctcc gtagaggttg agcgcgcggg
     3661 cgacttttca acctacacgc tccgcctcgt caagagcagc gacgatgcgg acacgccgga
     3721 cggtttcgat ccggttctct ccgccgtcga tttctccttc aaggtcgcct gtccgagcga
     3781 cttcgactgc gaggacgcga gcgcgtgccc gccggagcat ctgccgcagc ccttgatcaa
     3841 ctatctcgcc aaagattacg ccagcttccg gcagttgatg ctcgaccgca tgtccgtgct
     3901 catgccgcaa tggaccacgc gcaatcccgc cgatctcggg gtcgcgctcg tcgagatgct
     3961 ggcctacgtc ggcgaccacc tgagctacca gcaggacgcc gtggggacgg aagcctatct
     4021 cggcacggct cgccgccgca cctccgtccg acgccacgcg cgtctcgtag attaccgcat
     4081 gcacgacggc gcgaacgcgc gcgcgtgggt gcaggtgcag gtcgagggcg gcaacgtcga
     4141 actcgacaaa ggggcgcaaa tcttttcccg tctcccgcat cttccgcaaa gaatccagcc
     4201 cgcctctctg gactacgacg tggcgacggc ggcgcgtccc gtcgtcttcg agacgatgca
     4261 gaaggcgacg ctccacttcg cgcacaacac gatctttttt cacacgtgga gcgacgagcg
     4321 ttgctgcctg ccgaggggtg cgacgcgcgc cacgctcaga aacgaaggcg acacgctcgc
     4381 cgcgctcgcc gtcggcgacg tgctcatctt tgtcgagacg cggaacccgc aaaacggcaa
     4441 cgccgccgag gccgaccccg cccacagaca tgccgtgcgc ctgaccaaaa tcaagcccgc
     4501 caccgacccg ctctacacgg aggccgacgg cgtcgagcct ttgcgcgtgc tcgacatcga
     4561 gtgggcggct gatgatgccc tgccctttcc gctctgcctg tgggaggtgg agacgggcgg
     4621 cgaggcgaat gagaagacgc ccgccagcgt cgcgctcggc aacatcgtgc tcgcagacca
     4681 cggcctctcc atcgcgggcg aaactttcga cgccgttccc ccgcacgatc ccgcgctcaa
     4741 tcgtgccgca tctcacgggt cggattcctg cgcagaacga aagaccaggc cgtcgccccc
     4801 gcgctttcgc ccgcagcttg cacagcgtcc tctcgcctac gttgcgcctt tcgacccggc
     4861 acaaccgtcc atctctgcca gcgccgcgat gaactggccg gtgtcggagt ttgtcccggc
     4921 catcagcctg acgggcgcgc tgaacgcaca gacggccaca tggctgccac agggcgacct
     4981 ccttgcaagc gaggcgggcg cgaccgagtt tgtcgtcgaa gtcgagacgg acgggacggc
     5041 atacgtgcgc ttcggcgacg accgcttcgg ctcgcgccct ccaaccggca cgatcttcac
     5101 ggctgactat cgcgtgggcg gcggcgtcgc gggcaatgtc ggcgcgggcg cgctcgcgca
     5161 catcgtcacc aaccatgcgg gcgtcgtcgg ggtgacgaat ccgctccctg cgcgcggcgg
     5221 cgtcgcgccc gaatcaatcg agcaggtgcg acagaacgcg ccctacgcct ttcgcacgca
     5281 acagcgcgcc gtgacggcga aagactacgc cgaggtggct gagcgccacg cgggcgtgca
     5341 gcgcgccgcc gcgacgattc gctggaccgg cagttggcgc accgtcttcc tgaccgtgga
     5401 tagactcggt ggcgcagacg tggatgccga tttcgaacag gagatgcgcg ctcatctcga
     5461 acgataccgg atggccgggc atgacgtgga gatagacgcg ccgcgctacg tttcgctcga
     5521 aatcgaaatg acggtctgca tcaagccggg ctatttgaag agcgatgtgc ggcgcgcgct
     5581 ccttgagttg ttcaaccgcc acacgcgccc cgacgggcgg cgcggcgtct ttcacccgga
     5641 caattacagc ttcgggcaga ccgtcttcct cagtccgctc tatgccgccg cgcagtcggt
     5701 tgacggcgtg gactcggtcg agatcaagag gtttgcgcga cgcggggaga cgggcgacgc
     5761 tgcccttgag gcgggcaagc ttgagttgag ccgtcttgag atcgcgcggc tcgataacga
     5821 cccgaacttt cccgaacgcg gagtctttca actgactctg aaggggggac gatgagcact
     5881 gccttagacc cgaccctgca tcatctggac gactgcggct gctgcgaagg cgtggcggcg
     5941 cgcacgcccg tcgaaatttt caaccgaccg ggactctccg cgattgccta tcgcgcaggc
     6001 cgccacgcgc agttcaagca gacgctgctg gcgcgcctgt cgaacaaaga ccggcctgcc
     6061 ctcgcccggc tcaccacgcg cgacgcagac gacttctcca tcgcgctgct cgatgcgtgg
     6121 gcgaccgtcg ccgatgtgct caccttctat caggagagaa tcgccaacga gtcttatctg
     6181 cggacggcca ccgagcgtct ctcgctgctg gaactggcgc ggctcatcgg atacgagttg
     6241 cgtccggggg tggcggccag cgcgtatctg gccttcacga ttgaagaggt gccgggcgcg
     6301 acgcgcgaga tggcggcggc tctcggcgtg ccgcgagaga cgacgattga ccccggagtg
     6361 aaggcgcaga gcgtgccggg tcccggcgag cgcgcgcaga tgttcgaaac cgtcgagcgc
     6421 atagaggcgc gcgccgggtg gaacgagatc aggccgcgtc tgaccgaacg gcaagtggtg
     6481 aagttgaacg cgggcgcgct ctatttcgaa gggctggcga ccggactcaa gaagggcgac
     6541 gggctgctca tcaccgagga cgactatcac gagtctatgt ttcgggaagt ctcagaggtg
     6601 acgctcgaac ccgaactcac gcgcacgaag gtgcatctga agcctacgga actgcaaact
     6661 tttgccaaca tcaagattca actcggcgcg ctcacgacgg ctgtgaccag cgcggtgacg
     6721 cagaaattta tcgggcagac cgtccgcgcg cgcgacctgc gcgccagcgc gctcagccag
     6781 aagtttcaga tcgccgatct gttcgacaat atcctcgccg tccggcaacc gccgcccgcc
     6841 gtgctcgcgc tgcgaacccg cgcctccatc ttcggccaca atgcgccgcg ttgggagtcg
     6901 ctgcccgccg tgcagcgcat cggcgagtac gtggcggttg acggtggagt cgatccgtca
     6961 aacgtggagt tcaaaggggg cgtctacagg aaccgtgcca acagttgggc ggaggaatct
     7021 ctctacgatt accaccggct accgggcgaa ccggcgaaca agcacgacat ctatctcgac
     7081 gctgcctacc cgtccatcgt caagggtagt tggatcgtgc tcaagcagga tgatgatgcc
     7141 tacgcttatc aggtgaaggc gacgagcgaa gtctcacgcg ctgactacac gctgagcggc
     7201 aagtccacgc gcctgaggct cgaccgcttc accggcctcg gcaacttccg catccgccgc
     7261 acgaccgtcc acgcgcagag cgaggaactg gcgctggcgc gcctgcccat cgagactcct
     7321 gtggcgggca aggagctaga cctcgaaacg tgggttgacg gcctctccga aggacagagc
     7381 atcatcgtct gcggagaact cgacgacgca agcggcgtca acgcctgcga gcatgcgttg
     7441 atcaaggatg tcgagcacgt gctggagacg gaaggattca cgcggctcac gctcgtcgcc
     7501 ccgctcgaaa attcatacgt gcgcgcgacc gtcaagatca acgccaacgt cgcactggcg
     7561 actcacggcg aaaccgttac cgaaccgctc ggaagcggcg acgccgggcg cgtcttccag
     7621 cgtttcacgc tgcgccagcc gccgctcaca tacgtcagcg cgaagacgcc cacgggcagc
     7681 gcgacgaccc tcagcgtgcg cgtcaacggg ctgctctggc acgaggttcc atccttctac
     7741 ggtcatggtg cggacgagcg tatctacatc acacgcacgg acgacgacgg caacacgacc
     7801 gtgcagttcg gcgacgggcg cacgggcgcg cgactcccga ccgggcagga caacgtgacc
     7861 gccacctaca ggaaaggcac cgggctggcc gggcttgtcc gcgcaaatca actctcgcag
     7921 ttgatgacgc gcccgctcgg cgtgcggggc gtcatcaacc cttcggctgc gagcggggct
     7981 gccgatagag aagaacgcga cgaggcgcgt cgtaacgcgc ccctgaccgt tatgacactc
     8041 gaacgcgtcg tctcgctcgc cgactatgaa gacttcgcgc gcgccttcgc cgggatcggt
     8101 aaggcgcagg cgacatggac ctggaacggc gagcagcgcg gcctcttcct gacgctcgcc
     8161 ggggcgaacg gcgcagagat cgaaagcccg ctcgacaaaa acctcctcgc cgccctgcgt
     8221 gcgtcgggcg accccgcagt gccgctcgaa gtcgcttcat accagccgcg cctcttccgt
     8281 ctgaaagcga aagtgcgcgt ccacccggac tacctgccgg agaatgtcct gcgggaagtc
     8341 gggcgaaacc tgcgcgcccg tttctctttc gacgcgcggg cgtttggcca gcccgtcgcg
     8401 ctgagtgaag tcatcggttg tatgcagcgt gtggccggag tcgtggcggt tgacgtggac
     8461 gaactctacc gcacgggcga gacggccacc ttaaacacgc gcctgcccgc cgccgcgccg
     8521 cgcgccggaa gtcggcaggc cgcgcccgcc gaactgctca cgctcgatcc cggcgcgctc
     8581 gctctggagg tgatgcaatg agtttcgaca aggaaaaact ttacgcactg cttccggcag
     8641 tctaccgcct gcgcgacgac gagcagggca agccgctcaa ggctctgctg tcggtgatcg
     8701 cggagcaggt cgccgtgctc gaagaagacc tcgaacaact ctacgacgat cagtttatag
     8761 aaacctgtgc cgagtgggtt gtgccctaca tcggcgacct cgtgggcgca cgcgggttgc
     8821 agacgtggcc ggaggcgacc ttcagccatc gcgcgcaggt ggcgaatacg ctggcttacc
     8881 gtcgccgcaa aggaacggcg gcgatgctcg agcagttggc gcgcgacgtg acgggctggc
     8941 cttcaaacgt cgtcgagttc ttcgaacttc tctcgacgac gcagtacatg aaccacatcc
     9001 gcctgcgcca tcccgtcgag gttgatctgc gcggctggaa agctctgcat ccggccacgg
     9061 ttgacctgcg cgattgggaa gctctggaac ttctagacac tcccttcgag cgtctcgcac
     9121 acacggcgga cgtgcgccac atcgagacgc gccggggtct ttacaacatc cccaacgtcg
     9181 ccatttttct ctggcgtctc gccggttacc tgctgaccga tgcgcccgct caccggctcg
     9241 atgcgcgtcg cttccgtttc aacccgctcg gcttcgacac accgctctac aaccgcacgg
     9301 tcaccgaaaa agagatcacg cacctcgccg aaacggtcaa cgtccccgcg cccttgcgcc
     9361 gacgcctgct cgaacgcgat ctcgaactcc actacggcgc gaaccagagt tttcatctca
     9421 aggttgacga cgtgccgatt gacgccgacg ccatacgcat ctgcaacctc tcggacgacg
     9481 gcgcgggctg gctcgataag gcgaacgaca aacacgtcgt cgatcccgtg ctcggtcgca
     9541 tcatgctgcc cgaaggcgtc tacgccgacc ccgcgaaagt tcgcgtcagt tttcgctatg
     9601 gcttcagcgc cgagatgggc ggcggccaat acggtcgcgc ctcaaccttc acgaacgatc
     9661 tcgaaccgct gctcaaagtc cccacccacc atgcgatgct acaggacgcg ctcgacaacc
     9721 tcgacggcaa cggcgtggtc gagatcgaaa acaacgaata ctacttcgaa caattcttca
     9781 tcaacgccgc agccgacggc aaagtggagt tgcgcgcggc ggacgagcgg cggcctgtta
     9841 tcgcagcgac cggcgacttt tggatcacgg gcggcgacgg ctcggaggtg acgctcaacg
     9901 ggttggtgtt agccgggggc actgtgcgcg tgccgcttga gaagccgggg ggacagccga
     9961 atgaactccg tgtgctacgc atcagccatt gcactctgct gcccggcgcg acgcccgcca
    10021 tcgggaacgt gcccgcgcaa agccctacgg ttcgcatacg cattgacgcg ccgaacgtca
    10081 cggttgagat tgaccgttcc atcgtcggca tgattcgcgc cgtcgaagga gcgcgcgtcc
    10141 gtatcaccga cagcatcctc gacgcgggcg cggaaagcag tccggcttac gctgaccctg
    10201 cgctggcggc gggcgcgtcg ctccacatct cgaactccac cgtcgtcggc agggttcaca
    10261 ccgagttgat cgaagcgtca aacacgatct tcatgtcggg cgcggacgct tccgaccctt
    10321 ccctgctgcc gctcagagtc gagcgtttgc aggagggctg cgtacgcttc tcttatgtcc
    10381 cgcccggctc aagagtgccg cgccagtatc attgccagcc gcttcaggga gacacggcgg
    10441 agcagcgcgc acgcgtgcgt cctctcttcg aatcgctcac ctacggcgac gcggcctact
    10501 gccagttaag cccgcgctgc gctgcggaga ttcgacaggg cgcagacgac gaggcggcga
    10561 tgggcgcgtt tcaccatctt tacgagccgc tgcgcgaggc caatctgcgt gcgcggctcg
    10621 atgaatatct gcgcttcggc ctcgaagccg gaatcatcta cgcgagttga ggcgggaacc
    10681 gtttatctcg cgcgtctgtg aacctctaac gctgtcagag gaaacctcac agtcacactt
    10741 ccagaagttt ttgccgtgtc ttggcgtgcc ggggtggaat cagtttttaa ttttcttatc
    10801 agcatagatt cggaggatag taatgaaagg tgatttcagc cgtagaactt ttgaccgtgc
    10861 gaaacgctat agcggcgtcc tcatgcagca ggggcgcgtc caactcgatg ccgactggaa
    10921 cgagcagcta gccatccatc aacaccgcac cgagacggag acgaaggacg tgatcggcct
    10981 ctgcggcgtc cctaaaaatt cggacggttt cagactccag ccggttacgc ctgcgaacat
    11041 gtcggccacc accgacctcc tcatcttccc cggacgcatc tacgttaacg gtctgctctg
    11101 tgaagctgat gccgaaccgg tcgccatcac gggcttcact ccggcgggtg cgattgtcgc
    11161 ccacatgagc gcagacggtc gcgccttcga gtccggccag tgggttgaga tcacgggcga
    11221 cgacgaagcg aagaccctac ggctgatcac cgccgtagat gccgcgcaga acagcctgag
    11281 ctttgacaag gcaccgccga actacactcc gagccagcac ccgcacctgc gccgtctcac
    11341 gacttatctc acgcagcccg acctgcctgc gcccgcgcaa gccgcatcct acggcgaaga
    11401 ggaagcggcc actctggagt tgggagagga atattatctc gcttacctgc atgtccgcga
    11461 gcagcacatc accgcgctcg aagacccgag catccgcgag aaggcgctcg gtggtccaga
    11521 cacgacgacg cgcatcaaga acgtctggca ggtcgaactg ctgcccgtcg ccattcccga
    11581 cgacggcgcg ctcagttgcg agacggagtt ccccgaatgg actgccctca acgccccgtc
    11641 caccggcacg ttgaacgcgc gcacctcgcc cgcagacatc ccgaccgacc cgtgccagct
    11701 cccgccgggc gcgggttatc gtcgtctcga aaatcaactc taccgcgtag agatacagaa
    11761 gggcggcgag cgcgacgggg cgaccttcaa gtggtcgcgc gagaacggct ccattcaaac
    11821 gaccatcacg gagatcaacg gcaatatcgt caccgtcgcc gatctcggca gagacgaagt
    11881 gctcggcttc gcgggcggcc aatgggtcga gttggttgac gacgaatcgg agctacaggg
    11941 cgagccgcgc ccgctcgcct tcatcaactc gattgatcta tcttcgcgcg agattacgct
    12001 cgacaccgat gccacaagcc tcgcggggaa gaaaaatctg cgcctgcgac gttgggacca
    12061 gtcgggcggc aaggcgggcg cagacggcct cacgatgtct gatgattggc tctcgctcga
    12121 aggcggcgtg gaggtgcggt tctccgacgg cacgtatcgc gcgggcgatt actggttgat
    12181 cccggcgcgc acggcgacgg ctgacatcga gtggccgtcc gactccgcct ccggcgatgc
    12241 tcctttaccg caaccgcccc atggcgtccg tcaatactac tgccgcctcg cgctgtttcg
    12301 cgtagacgat gccatactct ggctcgtcga agattgccgc gatttcttcc ccgcgctcac
    12361 ggacatctgc gccgaagatg tctgtttcga taattcaaac tgtgacctgc cgaacgccga
    12421 gacggtgcag gacgccatcg aagcgctctg cggcgcaaac gatctgcgtt tccacaacaa
    12481 gcacctgcac ggttggggca tcgtctgcgg cttgcaggtc aactgtggtc cgaacgagcc
    12541 ggagtcgccg cgccgttacg tcaccgtgcg ctccggctac gcgattgact gtgaaggcaa
    12601 cgacatcatt cacagaaacg acgaacccgt caacattctg gagatgattc aagcctacaa
    12661 cttgcagcat ccgaacgcgc ccatcttgca gggcgacggc gaagtctgtc tcgtcctcaa
    12721 taccgatggg cagaatccct acgctctgga gaaatacgac cccgcatgga agtcttggaa
    12781 gtcggcgctg gaaggcacgc tcttcaagga tttctttcag gactgcctcg gcaacctgct
    12841 cctgttcttc aaggaggaac tctcgcccaa gcccggcgaa gcaaatctcc tcgtcagccc
    12901 cacgcagaaa ctcatcacgt cgctgctcaa tctggtggtg caactcttca acccgaccaa
    12961 tggcaggtat gtctacctct ccggcgagca gaacgtggag atcgacagca cggaacatac
    13021 catcctcaaa aacttttatg agaagttgcg cgggctgctc cagagtcaca ccttctgcgc
    13081 catgttcgat caggcgacgc cgttccccgt ctatccctac agcatgtaca acggctcgga
    13141 cagcgtgccc acctacatcc cgaccatctt cggcaaaggc tttcatcgtc gcctgcgcgt
    13201 gcatcccggc gggaagctcg cctatacggt cggcgcggcc aacaagattc acgtctacga
    13261 ccttgagaga aatgagatgg ttgaggagtt cgagtacccc ataggcaacg ccgtcgtcag
    13321 agacgtggcc ttctcgcccg acggtaacca attgtatgcc atcgccaccg tcaacaataa
    13381 ggagagcagt ttctccgtct tcgacgtgca gatcggctcc gggtcggtca agcacgtctg
    13441 gcgcaagaaa gggattctct gcgactttct cctcgtcacg ctcgccacgg cagaggacgg
    13501 caaagtctac ggcgtaggcc gtggccgtgg tctgtacatc ctccacatgg agaagtccga
    13561 gccggacgcc acgctcttcg gccagtcatt caacgcgagc gggcatctgg tggtcgtgcg
    13621 gcgcggggcg cagcagtttg ccttcgccac cgcgcaggag ccggggaact cgctcgacca
    13681 gtacaatcat gtccgccgtt tcaacctgct caacgcgaac gaaaatccga tgttcttgca
    13741 gctacagatt caggggcaga gcgtctccgg gcaggacgac atcgccgtcg cttccgacga
    13801 taagaacgca aagctctacg tcgtcacaaa cccgccctcg ttctcggaca acaatcatct
    13861 catcatcttc aacaacgcgt tttcggcctc gcccccgacg cagcagttca tcaacctcga
    13921 agagaagacc ggcatgcgtc tggcctacaa ctacccgacg cagaatttga tgatcaccta
    13981 ctccgacagc tatcaggtgc ggctcgtcgg gcagaacaac cagttggtcg cagggcaccg
    14041 gcaggccgtg cagatcgcgc ccacctcgat agccacgacg ccagaccaaa agcgcgtcct
    14101 cgtcctcaac tacgggagca atacgatcac ctcgttgcgc gccgacctcg ttaaggccaa
    14161 ctctttcccg ccgctcgaag gcgtctccgg tctgggaccc tatcgcgacc agatacttct
    14221 ggccttcgtc gatctcttcg gcgggctgct gcaatacctg aaggactgcc tctgcgacca
    14281 cttcctcgtt gactgtccgg agtgcgatga ggcagacaag atttatctgg cctgcatcag
    14341 catcaggcag gaggaggtct atcaagtctg caacttctcc cggcgcaagt acgtcaagag
    14401 tttcccgacc gtcgaatact ggctctcgct cgtgcccgtt attcccctcg tcggcaaatt
    14461 cgtggagcag ttctgctgca ccattctgcc ggagttcttt gtcggtaaat acaaaacctc
    14521 ggctgccgcc aacgccgccg cgccctcgca gaacaagttg agcagcgagg ccatgtacaa
    14581 gatttccagc gtgctgcaac agaccagttt cggaaatatg tttggcgacg cgctcaaaaa
    14641 gttcggatgg atcggcggtc aactcatcgg cgactggttc gagtgcgttt ttaaacgaca
    14701 gaacttacct tccggcgaga tagatcgagg tggggaaggg tcgccgggaa cgaccgggat
    14761 ggcgcagtta tccgaaaacg aaatggagcg cgatccctgc aactggtgcg gcaatcttgt
    14821 tcgctatatc tgcccgccgc gcactcaggg tgaaggccgc gacgcgacag gagcgagcgt
    14881 cgaaaccgat tttagacggg atgcgacttt taccgctgcc tcagccgaga ctgtgttgat
    14941 ggaaagaact gcgtccggtt cgcagaccga aggcgtctcc gccagtgagg atgtcttaag
    15001 tctgcgcaac gagatggccg ccctgcgtga agagttcgca cgcacgcaag ggagctacgc
    15061 gggcgcgttg gccgcacgcg acgccaccat cgcctcgctc gaagccacca cacgcgactt
    15121 acaggccagc cttgcgaccc tcagcgaatt acaggagaag gtcaactcgc tgacacccac
    15181 acgccgcaag ccgcgccaga gaaccaaaaa agaggacaag tccgcgcaat aagagctgcg
    15241 cccacgcagt ttcagacgaa gacaggcaac agccgcccgg cagacccaga tagacgccga
    15301 ttcaaactca tttttgatcc gcgtctttcc gcgtccatcg gcggcttatt ctttgacgca
    15361 tcggcctgac ccctcaaagg tcttaacccg tcgcccaact caacagtcac aaccaagaac
    15421 gctctgcgca aaagataagc attctgttca atatctgcta actgctatgg gtagccctaa
    15481 agacaccatg aacctgattc ccgcttgcta caattgtaat ctttggaagt tcaacttaaa
    15541 cgcctaattc cctgctaaat aaaggctaag agaaatccgc gcatgcgcta acattttctt
    15601 gactattttc agccggatcg gatataaggg cgggcatggc tgaattgaca acatcgcaag
    15661 tcgccgcgct cctcggcgtc ggcgcgtcca ccgtgcgact ctggtgtacc ttgaagcgat
    15721 tcccaagtgc gtacgaggtg caatcgcccc gaggcccgtt ctggctcatc cctgagggag
    15781 acttggaagg attcgtcgtg ccaaagcgcg ggaggccgag gaaatccact ggcttagtcg
    15841 ctacggtccc gccagccgag gaggacaaag ccgcatgaag accatcgcca tcgcaaacca
    15901 aaagggcggc gtcggtaaga ctacaaccgc agtgaatctc gccgcctgcc tcgcccggct
    15961 cgggcggcgc acgctcctcg tcgacctcga cccgcaggcc aacgccacag accatctcgg
    16021 cttcgaaaat ccggagggcg agttgcatag ctcctacgcc ttgatctctg aaaaaatgcc
    16081 cgatatgtca agcatcttgc gggctgtcgg gccgaacctc cagatcgccc ccggccacat
    16141 cgcgctcgcc gagattgaca tcaagctgtt ctcttccctt aaccgggaga cgcgcctgct
    16201 acgcgcgctc agtcaggtcg aaaatcagtt cgatttcgtg atcatagatt gcgcgccgtc
    16261 gctcggcatc tccactgtca atgccttctg cgcctctacg cacattatta tcactattca
    16321 aaccaactgg tttgcctacg aagccctgaa gcgcctgatg gcgatcgcct cggacgttat
    16381 ggaagaatca aaccccaatc tggagatata cgcgttggcg acgctgcacc gggcgaacgt
    16441 aaacgtcaat cgtgaggtgc tacagaaaat tcaggaagac ttcggaggac tagcaatctc
    16501 tgcggtgatt ccccacacag ccacgctcgc cgaagcatct gtggccaaac agccgatcat
    16561 cgactacgcc cacgggagca aaggccacca ggcttaccag aaactagccg aggagataat
    16621 tagccgtgtc gagcgacaac aaaccgaaga agcgcaagtt tcctgaaatt ccgagcgtgc
    16681 gcgcgacgcc gggcgagacg acggcgacgc agaacacggt caagcggatg ctgggacggt
    16741 tgccgctgga gcgcgaagga actgaggtcg agccgcaccc agcatcaccc accaaccacc
    16801 caccaaccac ccaccaacca cccaccaacc aacccatcgc acccgaaaga aacttctccc
    16861 gtgtgccaaa ctcgatagcc ctctctggca tcccctccgg tctttttcgc ggcgagagca
    16921 agaagttgta tgacgccctc tatcaaagga cacgcggcgc ggtcgtgccc cgccgggcta
    16981 tcagagccac ccaaagcgag ttgatgggat gggctaacat tagcctcaat actttgaaag
    17041 cgcatatccg gcatctgtca tcgataggcc tcctgaaagt ccattataga cgcggagaat
    17101 cgacgggcgc tgactatgaa atcttcaccc tggaggacag accacccacc aaccacccac
    17161 caaccaccca accaccaacc accaaccaaa atttgggatc acccacccac cagaatctgg
    17221 tcttgggtgg tgggtctcaa gtaactgtag aatcaacact tgccgcatct cccaagactt
    17281 cttttaagac taaagaagag aattctgatg atgacgctcc cgcgcgcact ttcctcgcca
    17341 aattggtcga ggccgaaagg gaactaacgg gcaaggcctc gacgagcggt gagaagtggg
    17401 aagagctagc cgaagtactt atctcggagc tgcggatcgc agccgcacgc acaaccgtct
    17461 caaacgtgcc tgcgttcctc gccgagcacc tgcgccgtcg cctctggaag gtggacaagg
    17521 cgcgcgccgc cgagatggcg gccagccagc ctgagcaaga aggcgttgcg cctgcactca
    17581 cgaccgagga gaagcagaag tgcccggact gcgccggtgt cggcttctgg tatccggaag
    17641 ggccggagaa gggcgtcgcc agatgctcac acctgaaact acattccctc ttagcaggac
    17701 agtgacccgc ctaagtaccg ccgcgtttca cagagctata cgacgcgcaa tttgttaatt
    17761 attgagctaa aaggcatgtc acaaggccaa tcaataaggc cagtcaaatc gaacgttcga
    17821 gataattgtc cttctgaaaa gtaactttat cttgaatcaa tgtccccgcg tcaccctgaa
    17881 aaagcatccg caaatttcgt ttcacatagg tttcacacac aaaagctctg ttttttctgc
    17941 aattaaaaag gttatttatg ttagcgagag ctaattccga taatgcttat tttacgaaat
    18001 caatttgact gcgttttcac gcgaggcatt tcggcgcatg cgcctcatct cgcacgacgc
    18061 tcggggcgaa gtcgaggacg cggggtaggc taacccggac atgaatttcc gcttcacaat
    18121 gttcgacccg gcgtcgcccg gttacatctt caacctccaa gcgaagggct tgtggcgcag
    18181 gcgtctacca tctcggcttc tccgctggcc gcgaccccat cgtctattcg gcagcgacgc
    18241 cgccgtctgt accgtgcagt tacagaccag gtaagagggg aaggggaggc cgttcggtaa
    18301 aactcgattt agcgtctggg cgtgatcttc cagagttcgc cattggcctc atccgtaacc
    18361 acgtagagcg ttccgtcgtg accttgacgc acgtcgcgga tccgctgccc gcgctctgtc
    18421 agcagatgct cttcgcccac gacgcgattc tctttgatga ccagccgcac gagccttgtt
    18481 gataccagac cgccgatgaa caggctgccg cgccactgcg gaaaagcgtc gcccgtgtag
    18541 aactgcgcgc ccgaaggcgc aattaccggg tcccagtaat agaccggctg ctcataacct
    18601 tcgcgcgccg tgacggctcc cgtgatcggc tcgcccgaat actcttcgcc gtaggtggcg
    18661 accggccagc cgtaattctt accacgctcg atcaggttca actcgtcgcc gcccttcgcg
    18721 ccgtgctcga ctgtccacag ccgcccacgg tcgtcgaagg ccgccgactg aaggtttcgg
    18781 tgtccgagcg tccatatttc aggcagcgcg ccggattgtt tggcgaaagg attgtcggcg
    18841 ggcaccgaac cgtcagggtt gatgcgaatg attttgccga gatgactgtt gagatgttgc
    18901 gcttgagggc gagtgattgt tttgtcgaag cggtcgccga gcgtgatgta gagcattccg
    18961 tcctgcccga aggcgagccg cgagccgaag tgcaggccgt tgttgtaagt cggcatcgcc
    19021 cggaaaatga tgcggacttg atcgagatta cgccggtctt cggttaacct gccgcgcgct
    19081 acactcgtcc cgctgccgcc ctcgcgctgc tcggagaagc tccagaagat ggtgcggtcg
    19141 cgcgaaaaat tcgggcttag agcgacatcg agaagcccgc cctgcccgcg cgcggtgatg
    19201 ggcggcagtc cgccctgccc gctcgcagcc gacacgccgc cggagccgac cggcaacagc
    19261 ccgccaattg gtgccccgac ctccccctta gcggaaacga tgcggagccg ccccggcttt
    19321 tcggtgatca ggagatctcc gccgggcagc ggctcgaccg cccacggcct ctccaagcct
    19381 ttcgccagaa cagtcacgtc gaaagccacg ccggacttca cggcgcaggc gcgagtctgt
    19441 ccggggaagg cgggtttctg ttccggcgcg ttcgccgttc ttgtttcgag cggcgcgcag
    19501 ggcgcgtttg aagttggcga agcgggcgtc gcagaagttt taaccgcatc gcgcccgccc
    19561 tgcgcgcacg cggtgccggt cgccatcaag acaatagcta gaacaaatgc cgtctgactt
    19621 tttttcatgt atgaccttcc taaaataccc cttggaattt aaagtttcag ttagtttgat
    19681 gacttctcag aaacagcgac gcgagccttc agggtcatgg cgcggcctgc ctccattcga
    19741 gtcgtccgtc tacgcgctgg ccgccgatac ggacgttttc gagcttcagc ccttcgacgt
    19801 tcttgaggac gaacgtgcgg tcggcctgct tgatgttgac gttgcgcagc gtaacgtcgc
    19861 gcaacggcgc ggctgccggg gcgtcgccgt tgaagaccgt gcccgcgcgt tcgaccgtga
    19921 agttctcgaa gacgatgtcg cggtaagttg acgggaagtt gccgccgagt tgaccgggat
    19981 agtcgagttg gaaccagaag aggttgtcga acgcctccac cgtgaagttg cgcacgcgga
    20041 tgtgttcgac cgtgccgcca cggtcgaggt tagccttgaa gcggatggcg gacgcgccct
    20101 tgcgcagtat gttgtctgtg aagtagacgt gacggatgcc gccggacatc tcgctgccga
    20161 gcgcgatgcc gtcctcgccg cccatgtcgt tgtggcggac gacgacgtgc tcgctgggtc
    20221 gtccgatctc gcggccatcg cggtcgcgcc ccgacttcac caccaccgaa tcgtcgccgg
    20281 tgcggaacca gcaacgctcg atcagcacgt ggcggcttga gtccacgtca acgccgtcgt
    20341 tgttggcatg gtggctgtcc acgcgtatgc gccgcacggt ggcgtggtcg gtgtagacga
    20401 ggtggttcac ccagaacggc gagttcagca ccgtgtaatc ctccagcagc acgcgctcgg
    20461 cgcggaagaa ctggatgatg ctcggcctca ggtgcgtgcc ctcgccaaag acgcgacggg
    20521 cgacgggcgt accgtccgcg cccatgcggc gcaggcgttg gaagtccggc tccgctctgg
    20581 gaatccaggt gagaaactcg ctcgcggcgt tgccgtcaat cgtgcccttg ccggtgatgg
    20641 caacgtcgcg cacctcaaag gcgtagatca gcggcgagta gctgtgcacc tcggtgcctt
    20701 cccaacgcgt gagcacgacc ggcaggtagt gccgcgcctc cgggctgaag agcagcgtgg
    20761 ctccctcggc gaggtggagg ttgatccggc tcgacagatg gacgggacca cggctcagcc
    20821 atttccccgg cgggatgacg acgcgcccgc cgccttccgc agacgctttt ttgatcgcag
    20881 ccatgatggc cgggcgtgcg tcgtccgtgc ccccggcctg cgcgccgaag tctgtgatgc
    20941 ggtagtcgcg gtcgggaatc gagggcagac ggatgctccg cacgacttcc tccacgcgtg
    21001 cccagtcgtc aaccggctga gcgagggcgg gcagggcgac gcccaaaacg agcgcgacgg
    21061 cgaagaatcg gacgtttagc gagagcgttc gggttcggtt cataacgtga ctcctaagac
    21121 ggtttcaggg ctgcggcgaa ttagcccccg cgatgaaatc cagcagggcg cggatgtatt
    21181 tagcgaacga atcaacctgc gggatgtgtc ccgcctcccc tatctcgacc agactcgcgc
    21241 gcgggatacg ccgtgcggca gccctgccga gtgcggggta gtcgcccatc gtggcggcca
    21301 cctctttcgg cacgcggtct ttgccgatgg ccgtccggtc gcgctgcccg atgatgagca
    21361 gggtcggggt tttcagtcgc tcgaattcgt agaggacggg ctgcgtgaag atcatgtccg
    21421 aggtgagcgc ggcgttccaa gcgacgcgtc ggtagtcagg atggcgcgtc cacccggcga
    21481 gaatctcggt caatgcgtca tactcaggct tccattcgcc gccgaagtag ttcacacgct
    21541 ggtactcctt aatagactcc ggcgtggcgc gcaactcctg tgcaaagttc tcttcgaccg
    21601 tgcggtaggg agccgacagt ttccagtcct caaggccgat ggggttgacg agcgcgaggc
    21661 gttcgacccg ccccgggtac atcagagcca tccgcgtcgc cagcatcccg cccatcgagt
    21721 ggccgacgag tgcgaactta tccacgcgga gagagtcgag cagcgaacgc gtgttggcgg
    21781 ccaatgcctg aaagctgaac tggtaggagg cgggcttgga cgacttgccg aagccgatct
    21841 ggtcgggcgc gacgacgcgg taaccggcat cggatagagc cttgatcgtg ggcgaccagt
    21901 aagcgccgga gaagttcttg ccgtgcagga gcacgacggt gctcccgttg ggtctggcgg
    21961 gcgcgatctc catgtaggcc atgcgcaacc tctggccttg cgtgtcgaac tcgtagaagc
    22021 tgacggggtg agggtactgg tagttggtca actccgggtc gagcgcaggc gtcgctgcgc
    22081 gtccctgagc ggcgggttta gtctcctgac cttgacccga cgccgtgagc accgcgagcg
    22141 tcaggacgaa cgacacgagc agtaggtaaa cgtctttcct cacttcgacc cccttggacg
    22201 taaaaagagt gtgtggcgtg aagcataact gatatgagaa tcgatctgcc agcagggatg
    22261 aggccgacgc cggaagccgt ctccgaaagg cgttggcgcg ggttgcggct aaactaatcg
    22321 aggttgagca accacacgtc ggcctctctg atggcaagtc cgtagtagag catgcgattg
    22381 tcggtcgaga ggccgtagcc tgagacacgg tgatgagatg gaggaaagag ttcgtgagat
    22441 tttttcgtct cggtgttgac gagatatatc ttgtgctggt ggttgaagat cagccgccgg
    22501 ttatctctga gccagacggg gctgtagccg aagtccgtga tccactcgaa gtcgcccgtc
    22561 tcgaaattgt agagcacgag gccgggagtc ttgccggacg ggcttgacca cccggcgagt
    22621 ttgcgcccgt ccggcgacca ggagttcacc cagtagtccg acagacggcg acgggtgtgg
    22681 tcgatcaggc gatagggtgc ctgctcgttc cagggcttct cgacctcgat gatggagcag
    22741 gattcgagat ggcgattgta gccgatgcgc gtgccgtcgg gcgaccagaa cgggtaggcg
    22801 atgctcgacc cttcggtgaa ggttatctgc cggaggtcgc tgccgtcggg tctgatcatc
    22861 caggcttcgt atctgccgct gcggtcggag tagaagacga tgcgcgtgcc gtccggcgac
    22921 cagcgcgggc tgcgatcctt gtagagatcg tcggtcagct tgcggggcga ctccgcgccg
    22981 tctttcccga caacgtagag gtcttcctgc ttttcgccct gtgaggagta gacgaacaac
    23041 tcgccgtccg gcgacaggtt cggcgcggac gcgtgccggg agccttgcgt gactgcgacc
    23101 ggctgccccg tgatcgtctc cttgtaaggg ttgaagccga ctttgaagat gttggtgctg
    23161 ctcacctgat tgacgtaggc cgcgcgccgt ccatcgcgcg agaaggccaa ctggtaggcg
    23221 taagatgaag gtgtgttcag cggttcgggc gctccggtcg cacgcccggt ctgttcttcc
    23281 agaggcacgc gccagaggtt catgctgccg ccccggtcgc tggcgaagta gagaaacttc
    23341 ccgtcgggcg accagaccgg gttccagtcg aaagccgcgt cgtccgtcac gcccacgggt
    23401 tcgctgtcac cgccgccgcg cgccgggact gtccagatgt cacgctgccc gcccttgtgc
    23461 ttgccccagt aagcgatgcg cgcgcccgaa ggcgaccatg cgggctgcgc cgcgtcgccg
    23521 gtcttcgcgt ccgtcagttg gcgtcgctcg cccgtcgaga cgttgacgat ccagatcatg
    23581 ctggagccga ggccacggac gttcgggtct gtaaaaccct cggtcgagaa agcgacctct
    23641 cgcccgtccg gcgaccatga ggggtagaag ccgaaatcgg tcaggcgtct tgttgattcg
    23701 cccgtcgcgc ccatgatgaa aatgccgccc ttctcccttt cggaacgaaa gacgacgtat
    23761 tcaccgtccg gcgataaggc gggatgggtg tcgtctgcgg gcgagttctc ggtcaggttg
    23821 agcggattgc tcccgcctac tctctgccag tagatgtccc aatcgtcagg cgcgcgcgcg
    23881 gcgtaaatga gagccttccc gtccggcgag agactcgggt agtgttcctg ccccggctga
    23941 gtcgtgagtt gcgtgacgtt gaaacgtgcg cccgccacgc ccgccgcttc tctcgcaggg
    24001 gctacggcgt tgcgacgcca cgcgaagtaa acaacggttg ccgcgacgac tattaagaga
    24061 gatgcgagcg cgaaccgggc tgtgcgtcgg gatagacttc gcggcgggcg cgtccgcgct
    24121 gcatccgcga cgaacttatc cgacacctgc ggcgacgaat cgagcgcgcg ttgcagacgc
    24181 tttagttcgg tgcagaggtc ggaggcggtt tgatgtcgcc gttcccgatc cttttccagc
    24241 gcgcggcaga cgacgcgttc gagatcgggc gcggcgtcgg agttgggtat gttcggcgtc
    24301 ggcgggtcgt ggttgaggat gcggtcgaac gtggcggcgt tcgtcaaccc acggaaaggg
    24361 tgtgtgcccg tcaacatttc gtaaaggatg acgccgaggc tgaacacgtc cgtgcggtgg
    24421 tctactttct gccccaaagc ctgctccggg gacatgtatg cgaccgtgcc tacgaccgtg
    24481 cccgtctccg tctgcaccga ttccgctaga tgatgcgggg cgtcaggcga tgagctgcgc
    24541 tcggtcaatt tcgcgatgcc gaaatcgagc accttcgcat aaccatcggg gcgcagcatg
    24601 atattttcgg gcttgatgtc gcggtgaata attccggcgg cgtgcgctgc gacgagtgct
    24661 tcggcgacct gagccgcgat gctgagcgcg tctgcgttct gcaactcacc ggcggcgatg
    24721 agacggcgca gcgtccgtcc ttcgacgaac tcggtggcga tgaagtgtgt gccttcggac
    24781 tggccgacct cgtggatggt gacgatgttc gggtggttca gcgcggaggc ggcgcgcgcc
    24841 tcatgctcga agcgacggac gcgctccggg tctttcgtga atccggcggg gaggagtttg
    24901 agcgcgacgc ggcgtttgag cctttcgtct tcggcgagat agacttcgcc catcccgccc
    24961 gcgccgagga gcgataaaat tctgtacgag ccgacacgcc gccccgcttc ggcagtgtcg
    25021 cccccacgcg ccgcagccct cgcgacgact tccaacgcgg gctgctcgat gaagttttcg
    25081 gcgcgcccgt gcgccgcgag cagcgactcg atctccgcgc gcagttcatc atcgcccgcg
    25141 cacgccgcct cgaggaaggc ggtgcgctct cccgcgtcct tctcgaacgc cacatcgaga
    25201 agcgcgtcta tctgcctgaa tcgttcagcc cgcaatgccg ccgccctctt cttcccccgc
    25261 gctcaattcg cggtagagcc acgccttcgc cttcgcccac tcacgcatca ccgtgacggg
    25321 agagatattc agaaattgcg ccgtctcctc gacggagagg ccgccgaaga aacgtaactc
    25381 cacgatctta ctcttgcgcg cgtccagttt cgccaactct tccaacgctt cgtcgagtgc
    25441 gacgaggtct gtgtcgcgtt cgtcggaaac gaccagcaac gcctcgtcga gtgcgacgcg
    25501 ccgtgccgtc ccttcgcgcg ctttcgggcg gctgcgcgcg aagtctacga ggatgcggcg
    25561 catcaactgc gccgagacgc cgaagaagtg cgcgcgattc tcgaagcgcg cgtccttcca
    25621 gttgatgagc cgcgagtagg cttcgttgac gagtgcggaa gtctgaagcg tgtgtccggc
    25681 gcgctcgcgc cccatgtagc gatgtgcgag gcggtgcagt tcggcgtgga cgagcggcac
    25741 gaggcgttcg agtgcggcct tgtcgccgcc gctccacgcc gccagcaact ctgtcacgtc
    25801 atgtgtcgaa ggatcggcca tagaaagatt tacggagagg acttggaacg ttaacacgtg
    25861 ccggggaaca aaatttatca cacatcgcgc tggcgtctcc aactcttccg accgccggag
    25921 aaatatttta cgggcaggtg atagttttcc cgcacgccgc acgcaatagc ttgtcagagg
    25981 agaaactttg acatgacaag aaacaggatc gctaaatctt tactggtcgc cgtgctgctc
    26041 ggcgcactcg tcgccgcgtt gtctcccttt aactccgtcc cgaatacatc ggcgcaggca
    26101 aagcagcccg cgccgcagag taagacgaaa ttccgccgca cgcgcgtcac cgccgcagac
    26161 atcgcgcgcc tgccgcgcgg caaaaactac gtcgtgcaga tcagccccgg cacgcagagc
    26221 cgcgcgggca cctacagcgg ctcgacgact gtgaacagcg gcgttttgaa ctccagagac
    26281 gagggcggca aaagcggcct gaccaaagct ggcgcgggca cgctcacgct cgcgggcact
    26341 aacaacatct acgagttcgt ctccgacacg cccattgatt tcagccgcgt cgtggtgcag
    26401 acaagcccgg acgcagcccc ggagtcgctc gaagcgtggc tgcggaagca tcgtccggcc
    26461 agcgggatga gaggctggcc tttcaggcgt ctgctcatcg gctcggctga agggatcgcc
    26521 gaagtcgagg gctggaaaat cagagacgcc gcacccggca ccgagtacaa atgtgagagt
    26581 gggggcgaag gcgacgacta ctgcggctgt tccagcattc tcgactgcac cctgatggtg
    26641 gttgcgggca agtgctcttc gtcactctcc tgcggcgacg gcgaatgtta ctgcgacgcg
    26701 cgctgatgca cggcttctct ctcgcggagg caaacacatg aaaatgaacc ggagcggcgc
    26761 gcgcgccgcg ctgctcggcg cactgctgct gtacgcggcg tgcgctggcg cggcctctga
    26821 gcatctcatg ccagcgtcgg aagccgaggc ggttgcggct gtggcgcgca cggcgctcgc
    26881 gcgactcccc gccgaggcgc gtctggacga gatcagggac gggcatctgc gccgcgccgt
    26941 gcagacgaca tatcgcgcgg tcgtcgaact ggccgacaac cgcgactccg ccagggtcgc
    27001 cgccctcaac tccaaattcg agcgcgccta tgcttcagtc gcgcgcgaaa tcgcgcgggg
    27061 cgaacaccgt acctgcgcgg ccaactgcaa gtcgttcgac ggcgacccgt gcgtggcgaa
    27121 atgcaaggcc gacggtaaaa agttttgcgg ttgcaagttg atcctcttcg gctgcgtcgt
    27181 cgcggagtgt ctcttctgaa ggctgtcggg cgagccggga gcgttgcacg acggcaggcg
    27241 tcgtttgtcg cttcgccgtt cccggtcgcc gctcagcgac tttgcactcg aagcactaac
    27301 gcgccccaaa aatagttctc gcaacacaac ctccggctgt gctctgacaa cctccggctg
    27361 tgctctgaca gaagattgac cggcggctct catcccttcc tatccagtcg ctatctctcc
    27421 cgcttccctt aacctgactg ttttcatcag cggcaacgct cgtgcgaaga atgccctgcg
    27481 tgcggtcgct agtgtggcaa aattttccat gcagtaaaat atattgtgat aaatgagtca
    27541 tcgcagaggc tgcatatcgc ggctaaaatt ttttttgtgc gacacagcct gtctgtgaag
    27601 gattagagaa gaaccttcga cgtgttaatt tatgaagcga tagccctgtc ggaattgcag
    27661 attcggtggg catccatatt gatcggcgag gcgcgagcgc caactcttaa ttcgccggag
    27721 tcccatgcaa ggagaaagaa tggaacgtaa agtagcatcc gattacccgc aggagttgtt
    27781 agacctgttt cacgaatacc agcacggcga catagaccgt cgcactttcc tctcacgcgc
    27841 cggtaagttt gcggtcggcg gcctgacggt cgcggcaatt ttcgagagcc tgacgcccaa
    27901 ctacgcctgg gcgcagcagg tgccgccgga cgacaaacgc atcaaggtgg gctacgaagt
    27961 cgtgcaatcc cccgccggaa acggctcgat caaggggtat ctggctcgtc cggcacaggg
    28021 gaaaaagctg cccgtcgtgc tcgtcattca cgaaaaccgt ggactcaatc cttatatcga
    28081 agatgtcgcg cgtcgcctcg cgctcgccaa gttcatcgcc tttgcgcccg atggattgac
    28141 ttcggtgggc ggctatcccg gcagcgacga gaaaggcgcg gtcgccttcc gcacggtgga
    28201 cgggaagaag atgaccgaag atttcgtcgc ggcggccaaa tggctcaaag cgcgtcccga
    28261 ttccacacgc aaactgggcg cggtgggttt ctgtttcggc ggcgggatgg tgaatcagtt
    28321 ggccgtgcgg ttgggcgcgg aactgaatgc cggggtcgcg ttctacggtc ggcaggcggg
    28381 cacggctgat gtccccagga tttccgcgcc gctcttgttt cattacgcgg gcaacgacga
    28441 gcgtatcaac gagggcatcg cggcttacga ggcggcactc aaggccaaca agaagattta
    28501 cacctcgcac atgtacgagg gcaaacagca cggcttccac aacgacacga cgccacgtta
    28561 tgacgaagcc gccgccaaac tgtcatggac gcgcaccctc gaattcttca acaaacacct
    28621 gcgctgacgc ttgacc
//
LOCUS       DASWNS010000294         9707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_321828, whole genome shotgun sequence.
ACCESSION   DASWNS010000294 DASWNS010000000
VERSION     DASWNS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321828"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1592
                     /locus_tag="VGB76_10400"
     CDS             78..1592
                     /locus_tag="VGB76_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0099348.1"
                     /translation="MLELRDITKRFGNVLANDGVSIKVAPGTIHAIVGENGAGKSTAM
                     RIAYGFYTADGGDILVDGEVRRISTPHDAIALGIGMVHQHFMLVEPMTVAENIVLGAE
                     TGGVASLDLGKAAREIRALSDEFKLSVDPNATIDSLSVGQQQRVELLKALYRRARLLI
                     LDEPTAVLTPQEVEEFFAILRRMREQGKTVVIITHKLSEVLAISDDVTVMRDGRVVGR
                     VRTSETNAAELARLMVGRDVLLRVEKTEARTGANVLSVHNLTLTGRDGARRLDAVSFK
                     VGAGEIVGIAGVEGNGQTELIESLAGLVAGNNALTGEVEFDGRNIARLSARARRELGI
                     AHIPEDRHRRGLLLDFDLAENSILGDHYRKPAVAGLGDVFLNDRGIRTRALEVIKDFD
                     VRPANPDLPARSLSGGNQQKLIIGREFGLKPKLLLVSQPTRGVDIGAIEFIHRKLVAL
                     RDAGCAVLLVSAELEEVTALADRLLIMREGRIAGEVDPRQATIEEIGLLMTGGH"
     gene            1746..2861
                     /locus_tag="VGB76_10405"
     CDS             1746..2861
                     /locus_tag="VGB76_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0099349.1"
                     /translation="MKLLRELFFPLVAVLAAFVLGGIFVWIIGDNPFETYRLLIGSAF
                     SWPDGIGYTLFYATPLIFTGLAVAVAFRCGLLNIGAEGQLYVAAFATAWVGIKFGGTV
                     VDVFGTPVNYSWMTLPAILLIPLAIVTAIATGAAWGAIPGFLKARFGSHEVINTIMLN
                     FIAIALVSYFTQYHFKQAGDPIMQSVPVGAGVHLPRLGRFVPGLPERIPLNLAFLLAL
                     LACVLVYLFLWRTKWGYAIRATGANPTAAEYGGISTGKQIVLAMAVSGGLAGMVAINE
                     VLGYRYRYYDGFSANYGFTGIAVALLGRNHPVGVLLSALLFGMLIRGGIFVDAFSGKV
                     SKDLVEVLQAIVILFVAAEAFFRGTFNRFGLLKRSKI"
     gene            2960..3850
                     /locus_tag="VGB76_10410"
     CDS             2960..3850
                     /locus_tag="VGB76_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0099350.1"
                     /translation="MSELFTIALIYSTIRTATPLLLAALGGLYSERAGVINIALEGLM
                     LAGAFTAAAVTHYAGNPWIGLLAGMGAGVAVAAIHAVACIRFNADQVVSGTAINILMT
                     GVPAMLSGALFLSSGSTPQIPKDNLIPGTPVVLALLLIGVTWWVLYRTPYGLRLRAVG
                     ENPEAADAAGVNVRGVRYSAVLLSGALAAIGGAYLSIGQSSLFTRNMTSGRGFIALAA
                     LIFGKWRPVQTMLACLLFGFTEAVSIQMQGVFKLPSGEDIPVQFIQIVPYVLTIVVLA
                     GFIGHSRAPRALGVPYQKEK"
     gene            3977..4822
                     /locus_tag="VGB76_10415"
     CDS             3977..4822
                     /locus_tag="VGB76_10415"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY0099351.1"
                     /translation="METSSGTTSTLYERAEHAARTIRALRAEEPRVGLVLGSGLGAFA
                     DDLEDAIAIPYEEIPGFARSTVEGHAGRLVLGKVGGVPVVVLQGRFHFYEGYTLEEVT
                     FPIRVLGLLGVKVLVLTNAAGGLNNSFSQGALMLISDHLNLMGTNPLLGANDARFGTR
                     FPDMTEVYDREFQEATIAQAREMKLELKRGIYAALTGPSYETPAEIRMLRALGADAVG
                     MSTVPESIVARHMGLKVLGISCITNMAAGVLDRPIDHAEVMETGEAVRATFTELLRRT
                     IPKLQ"
     gene            4925..5584
                     /gene="udk"
                     /locus_tag="VGB76_10420"
     CDS             4925..5584
                     /gene="udk"
                     /locus_tag="VGB76_10420"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEY0099352.1"
                     /translation="MIIGICGGTGSGKTTVANKILQSVSANEVAFIQQDSYYRNLDQM
                     PLDFRQKVNFDHPDAIDNDLMVEHVRALKSGRAVDLPIYDFKNHLRRKETLRMEPKLI
                     TILEGILIFAEKRLLEEMDIKVFVDTPDDIRFIRRLRRDIAERGRTADSVIEQYLATV
                     RPMHMQFVEPSKRCADVIIPEGGHNKVSIGLLSGTIRERLADEQQRRGIISSANAKIG
                     S"
     gene            5608..6030
                     /gene="cdd"
                     /locus_tag="VGB76_10425"
     CDS             5608..6030
                     /gene="cdd"
                     /locus_tag="VGB76_10425"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY0099353.1"
                     /translation="MSTTEVQEAIIEAATGARNCALAQFSKFCVGAALEAEDGTIYTG
                     CNIESAAYELTMCAERVTIWKALSEGARKFKRIAVVADTERLTPPCGACRQIIWEFCG
                     DVPVVLANLRGDTETLQMAWLLPRPFDAKFLDETSDEK"
     gene            6224..7753
                     /locus_tag="VGB76_10430"
     CDS             6224..7753
                     /locus_tag="VGB76_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0099354.1"
                     /translation="MKPSRIFVRPLLVVLLACAFVNSSVAHVAAFAPRSVDLVVRGGT
                     VVTMDERGSVFEDGAVAVERGRIVAVGKRADIDKSYAGRETIEARGRIVMPGLINGHT
                     HVPMTLFRGLADDLDLNEWLTKFIFPAEAKNVTEDFVRVGTRLGLAEMIRGGTTTYCD
                     MYYFEDAIADETAKAGVRGVLGETVIDFPVADNKTFAEGLAYTEKFIEKWRGHALITP
                     AIAPHAPYTVSEEHLKAVRDLSNRLRATIVIHVAETRKEVEDVTRDHGASPLAYLARI
                     GFLYDRVIAAHTVHLTTEEIELLKRQGVGAVHNPQSNMKLASGVARVPEMLRADVALG
                     LGTDGAASNNDLDMWEEMDTAAKLHKLTSNDPRVVPAREVLEMATIRGARALHLEREI
                     GSLEKGKRADLIVVDMDALNQTPMYNVYSHLVYATKAANVRTVVIEGRVVMRDRRLLT
                     LDEEAIKRDARAYRVRISQSLAPASVSPAGAKRDESDTSGTRKRKPATRPARKKPARR
                     S"
     gene            7850..9070
                     /locus_tag="VGB76_10435"
     CDS             7850..9070
                     /locus_tag="VGB76_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0099355.1"
                     /translation="MKESPLIRSTRTLAKVTFSLLLILAFAPGLRAQSSNDLDAEGIQ
                     RRITRARALAAAGNLPAARSELEALQRTAGVDPSVREITLVLLAGIYIDQADYTYAEN
                     LLNAAFGARTTGGESAARAYFVLAGQVLGGVRARLERYRVFGLNVADEALPAEASGDV
                     DRLRTLIEKVFAQGRQLRDENARNMDATALLEEAAGVRVRLARREDERALWQRELTDA
                     RQRLIGTTARAASNGTNAPNARTQGSSILAGSSQPANAAQPSPTPANAAPSTSTNAPN
                     AAQNTAANTQRGSSTATTTAPPNARPSATPAGGNATEGRPLEVGSLVPRAAQTVAPAY
                     PPTARSARVAGKVTVFLMVDEKGAVAAVERTDGPELLRRAAEEAARRWKFRPTIVNGQ
                     PVRVSGFINFNFTL"
     gene            complement(9521..>9707)
                     /locus_tag="VGB76_10440"
     CDS             complement(9521..>9707)
                     /locus_tag="VGB76_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099356.1"
                     /translation="NVRQTAAQMNAPAVRFDPFFRWLGVKFVCRFGSRDTKPCMRCVG
                     YLIGLSSFPCVRFAAAD"
BASE COUNT         1862 a         2851 c         3093 g         1901 t
ORIGIN      
        1 aactcttcgt tctctgcgtc tctgcgtcga gtcaggtttc ttcactgctc aaccggctgt
       61 tatagaaaat tcctttcatg cttgaactgc gagacatcac gaaacgcttc ggaaacgtgc
      121 tggcgaacga cggcgtgagc atcaaggttg cgccgggaac gattcacgcc atcgtgggtg
      181 agaacggcgc gggcaagtct acggcgatgc gcatcgctta cggcttctac acggctgacg
      241 gcggcgacat cctcgtggac ggcgaggtgc gccgcatctc gacgccgcac gatgccatcg
      301 cgctcggcat cggcatggtg catcaacatt tcatgttggt ggagccgatg acggtggccg
      361 aaaatatcgt gctcggcgcg gagacgggcg gcgtcgcgtc gctcgatttg ggcaaggcgg
      421 cgcgggagat tcgcgcgctc tcggacgagt tcaagttgtc ggttgatccg aacgcgacga
      481 ttgatagcct gtcggtcggg cagcagcagc gcgtggagtt gttgaaggcg ctctatcgtc
      541 gcgcacgcct gttgatcctc gacgagccga cggcggtatt gacgccgcag gaggtggaag
      601 agttcttcgc catcctccgg cgcatgcgcg agcagggaaa gaccgtcgtg attatcacgc
      661 acaaactctc ggaggtcttg gcgatctcgg acgacgtgac ggtgatgcga gacgggcgcg
      721 tcgtcggtcg cgtccggacg agcgagacga acgcggcgga gttggcgcgc ctgatggtcg
      781 ggcgcgacgt gctgctccgc gtggaaaaga ccgaagcacg aacgggcgca aacgtactct
      841 ccgtgcataa cctgacgctg acagggcgcg acggcgcgcg acgcctcgac gccgtttcgt
      901 tcaaggtcgg cgcgggggag atcgtcggga tcgcgggcgt cgaaggcaac gggcagacgg
      961 agttgatcga aagtctcgcc ggactcgtcg cgggcaacaa cgccttgacg ggtgaagttg
     1021 agtttgacgg gcgcaacatc gcgcgcctct ccgcacgcgc gcggcgcgaa ctcggcatcg
     1081 cgcatattcc cgaagaccgt catcgtcgcg gcctcctgct cgatttcgac ctcgcggaaa
     1141 attccatcct cggcgaccat tatcgcaagc ccgccgtcgc ggggttggga gacgtgttcc
     1201 tgaatgatcg agggattcgc acgcgcgccc tcgaagtgat caaagatttc gacgtgcgcc
     1261 cggccaatcc agacctcccg gcgcgctcgc tctccggcgg caatcaacaa aagctgatca
     1321 tcgggcgcga gttcggactc aagccgaaat tgctgctcgt ctcgcaaccg acgcgcggcg
     1381 tggacatcgg cgcaatcgag ttcattcacc gcaaactcgt cgccctgcgc gacgcgggct
     1441 gcgccgtgct gctcgtctcc gccgaactcg aagaagtaac cgcgctcgcc gaccgcctcc
     1501 tgatcatgcg cgaagggcgc atcgccggag aagttgaccc gaggcaggcg acgattgaag
     1561 agatcggttt gttgatgacc ggcggacatt gagagagcgt ctcaaaatta acttcgcatc
     1621 tttccctttg cgcctttgcc cctttgcgtg agttatattt tcaagtcggc gagagcctca
     1681 cgccgggacg cacgggcgca aagaagaatg gccggatgag gtttgagata gacgctaaat
     1741 aacctatgaa acttctgcgc gaactcttct tcccgctcgt cgccgtgctg gcggccttcg
     1801 tgctcggcgg catcttcgtc tggatcatcg gcgacaaccc gtttgagacg tatcgcctgt
     1861 tgatcgggag cgcgttctcg tggccggacg gcatcggcta cacgcttttt tacgcgacgc
     1921 ctctgatctt cacggggctg gccgtggccg tcgcgtttcg ctgcgggctg ctcaacatcg
     1981 gcgcggaagg tcaactctac gtcgcggcct tcgcgacggc gtgggtcggc atcaagttcg
     2041 gcggcacggt ggttgacgtg ttcggcacgc ccgtcaatta ttcgtggatg acgctgcccg
     2101 ccatcctgct catcccgctc gcgatagtaa cggcgattgc gacgggcgcg gcgtggggcg
     2161 cgatccccgg cttcctcaaa gcgcgtttcg gctcgcacga agttatcaac acgatcatgt
     2221 tgaacttcat cgcgattgcc ctcgtcagtt atttcacgca gtatcacttc aaacaggcgg
     2281 gtgatccgat catgcaatcc gtgcctgtgg gcgcgggcgt ccatctgccg cgactgggca
     2341 ggttcgtccc cggcctgccc gaacgtattc cactgaacct cgcgttcctg ctcgcgcttc
     2401 tggcgtgcgt gctcgtctac ctgtttttgt ggcgcacgaa gtggggctac gcgattcgcg
     2461 cgacgggcgc aaacccgacc gccgccgaat acggcggcat ctcgacgggc aagcagatcg
     2521 ttctcgcgat ggccgtctcc ggcggactcg ccggaatggt cgccatcaac gaagtgctcg
     2581 gctatcgtta tcgctactac gatggcttct ctgcgaatta cggattcaca ggcatcgccg
     2641 tcgcgctctt agggcgcaat cacccggtcg gcgtgctgct ctccgcgctc ctgttcggca
     2701 tgttgattcg cggcgggatt ttcgtagacg cgttctccgg caaagtctcg aaggatttgg
     2761 tcgaggtgct gcaagccatc gtcattctct tcgtcgcggc ggaagccttc tttcgcggca
     2821 cgtttaacag gttcggtctg ttgaagagat caaaaatctg agtcaggaga cagaattcag
     2881 aacacgggag tcggaattaa cgcgcgtgtg atccttaact tctgactcct gtattctgaa
     2941 ttccgtatgc tacgcattta tgagtgaact tttcaccatc gcgcttatct actcgacgat
     3001 acggacggcg acgccgctgc tgctcgccgc gctaggtggg ctgtattcgg agcgggcggg
     3061 cgtcattaac atcgcgctcg aaggattgat gctggcgggc gcgttcacgg cggcagcggt
     3121 gacgcattac gcgggcaacc cgtggatcgg tctgctcgca ggcatgggcg cgggcgttgc
     3181 cgtggcggcg attcacgccg tcgcgtgcat ccgcttcaac gccgatcagg tggtgagcgg
     3241 cacggcgatc aatatcctga tgacgggcgt gcccgcgatg ttgagcggcg cgctctttct
     3301 ctcttccggt tcgacgccgc agattccgaa ggataacttg atcccgggaa ctcccgtggt
     3361 gctcgcgctc ctgctcatcg gcgtgacgtg gtgggtgctc taccgcacgc cttacggttt
     3421 gcgcctgcgc gccgtgggcg aaaatccgga ggcggctgat gcggcgggtg tgaacgtgag
     3481 gggcgtgcgt tattcggcgg tgctgttgtc gggcgcgctc gcggcgattg gcggcgcgta
     3541 tttgtccatc ggtcaatcgt cgctctttac gcgcaacatg acatcggggc gcggcttcat
     3601 cgcgctcgcc gcgctcatct tcggcaagtg gcgacccgtg cagacgatgc tggcgtgcct
     3661 gctgttcggt ttcaccgaag ccgtctcgat ccagatgcag ggcgtcttca agttgccttc
     3721 gggcgaagac atccccgtcc agttcatcca gatcgtgccc tatgtcttaa ccatcgtcgt
     3781 cctcgcgggg ttcatcggcc actcgcgcgc accacgcgcg ctcggcgtgc cgtatcagaa
     3841 agagaagtga gagacttgcc ggggcggttc tacgttgcgg gtgcctcacg aaataagttg
     3901 cttgagtttg aattgatcaa ctgacggcaa gggaaagaac gaagggcaat tgaccacgga
     3961 ccacggacaa gctgttatgg aaacttcaag cggcacgacc tcgacattgt atgaacgcgc
     4021 ggagcacgcg gcgcgcacga ttcgcgcgtt gcgcgcggaa gagccgcgcg tcggattggt
     4081 gctcggcagc ggcctcggcg cgttcgcgga tgatctggaa gacgcgattg cgattcctta
     4141 cgaagagatt ccgggctttg cgcgttcgac cgtcgaaggg cacgcggggc gtctggtgtt
     4201 gggaaaagta ggcggcgtgc cggtcgtcgt cttgcagggg cgattccact tttacgaagg
     4261 ctacacgctc gaagaagtga cctttcccat acgcgtgctc ggcttgctcg gcgtgaaagt
     4321 tctcgtcctg acgaacgcgg cgggcggctt gaacaactcg ttctcgcagg gcgcgctgat
     4381 gttaatcagc gaccacctga atttgatggg caccaaccct ctgctcggcg caaacgacgc
     4441 gcgcttcggc acacgctttc cggatatgac ggaggtttac gaccgcgaat ttcaagaggc
     4501 gacgattgca caggcgcgcg aaatgaaact cgaactcaag cgcggcatct acgccgccct
     4561 cacggggccg agttacgaaa cgcccgccga aattcggatg ctgcgcgcgc tcggcgcaga
     4621 cgccgtcggc atgagcaccg tgcccgagtc aatcgtcgcg cgccacatgg gactcaaagt
     4681 gctcggcatc tcgtgcatca cgaacatggc ggcgggcgtc ctcgaccgtc ccatcgatca
     4741 cgccgaagtc atggaaacag gcgaagcggt gcgcgccacc tttacggaac tgttgagacg
     4801 gacaatacca aaactacagt gacgggtgac aggtgacgag tgacaagtta aaacaaaagc
     4861 cttgtcttga ttctggctcg tcacacgtca cctgtcaccc gtcacttatc aggcgaggcg
     4921 agggatgatt attggaattt gtggcggcac ggggtctgga aagacgacgg tggcgaataa
     4981 gattttgcag tcggtgagcg caaacgaggt cgcgttcatc cagcaggact cttattatcg
     5041 caacctcgat cagatgccgc tcgatttcag gcagaaggta aacttcgatc acccggatgc
     5101 gattgacaac gatctgatgg tggagcacgt ccgcgctttg aagtcggggc gggcggttga
     5161 cctgcccatc tatgatttca agaaccacct gcgccgcaag gaaacgctca ggatggagcc
     5221 gaagctgatc acgattctcg aaggcatcct gatcttcgcg gagaagcgtc tgctcgaaga
     5281 gatggacatc aaggttttcg tggatacgcc cgacgacata cgcttcatac gccgtctgcg
     5341 ccgcgacatc gcggagcgcg ggcgcaccgc cgattccgtc atcgaacaat atctcgccac
     5401 cgtccgcccg atgcacatgc agtttgtcga gccttcgaag cgttgcgcgg acgtgatcat
     5461 tcccgaaggc gggcacaaca aagtgagcat cggcctgctc agcggcacta tccgcgagcg
     5521 gctggccgac gagcagcaac ggcgcggcat catcagtagc gccaacgcca agattggtag
     5581 ttgagttgaa gaggagtaca aaaagggttg agtacaacag aagtgcagga agcgatcatc
     5641 gaggcggcga cgggcgcgcg taattgtgcg ttggcgcagt tctcgaaatt ttgtgtcggc
     5701 gcggcgctcg aagccgagga cggcacgatc tacacgggct gcaacatcga gagtgcggct
     5761 tatgaattga cgatgtgcgc cgagcgcgtg acgatctgga aggcgctctc ggagggcgcg
     5821 cggaagttta aacgcatcgc ggtggttgcg gatacggaac ggttgacgcc gccgtgcggc
     5881 gcgtgtcggc agatcatctg ggagttttgc ggcgacgtgc cggtcgtgct cgccaacctg
     5941 cgcggcgaca cggagacgct gcaaatggcc tggctcctgc cgcgcccctt cgacgcgaag
     6001 tttctcgatg aaaccagcga cgaaaaatag tctctctctc tctctctctg tgccttaagt
     6061 tttaactttg cgtctccggc gagagaaccg tctcacgccg gaggcgcagg gaagtagaga
     6121 cgctagacgc agagcgggcg gccaaaggtc agcttttcgc tctcgataaa atcttcttcg
     6181 agttaattcc ttcaagttaa tgatgactga aataacaggc gtgatgaaac catctcgaat
     6241 cttcgtgcga ccgttacttg tagttcttct cgcgtgcgct ttcgtcaact cttccgtcgc
     6301 gcacgtcgcg gccttcgcgc cgaggtctgt cgatctcgtc gtgcggggcg gcaccgtcgt
     6361 cacgatggat gagcgcggga gcgtgtttga agacggcgcg gtggcggtcg aacgtggccg
     6421 gatcgtggcg gtgggcaagc gcgcggacat agataaaagt tacgcggggc gcgagacgat
     6481 tgaggcgcgc gggcgcatcg tgatgcccgg cctcatcaac ggccacacgc acgtgccgat
     6541 gactctgttt cgcggcctcg cagacgatct cgatctgaat gagtggctca cgaagttcat
     6601 ctttccggcg gaagcgaaga acgtgacgga ggatttcgtg cgcgtcggca cgcggctcgg
     6661 cctcgccgaa atgattcgcg gcgggacgac gacctactgc gacatgtact acttcgaaga
     6721 cgccatcgca gacgagacgg cgaaggcggg cgtgcgcggc gtgctcggcg agacggtgat
     6781 agattttccg gtcgccgaca acaagacctt cgccgagggg ctggcctaca cggagaagtt
     6841 catcgagaag tggcgcggcc acgcgctcat cactccggcg attgcgccgc acgcgcccta
     6901 cacggtttcg gaggaacatc tcaaggccgt gcgcgatctc tcgaaccgtt tgcgtgcgac
     6961 gattgtgatt cacgtggcgg agacgcggaa agaggtcgaa gatgtaacgc gcgatcacgg
     7021 cgcttcgccc ctcgcctatc tggcgcgcat agggtttctc tacgaccgcg tgatcgcggc
     7081 gcacaccgtt cacctgacga cggaagagat cgagttgttg aagcgtcagg gcgtcggcgc
     7141 agttcataat ccgcagtcga atatgaaact ggcatcgggc gtggcgcgtg tgccggagat
     7201 gctcagggcg gacgtggctc tgggtttggg gacggacggc gcggcgtcga ataacgatct
     7261 ggacatgtgg gaggagatgg acacggcagc gaaattgcac aaactgacat caaacgatcc
     7321 gcgcgtcgtc ccggcacgcg aggtgttgga gatggcgacg attcgcggcg cgcgcgcttt
     7381 gcatctcgaa cgcgagatcg gctcgctgga gaagggcaag cgcgccgacc tgatcgtggt
     7441 tgacatggac gcgctcaacc agacgccgat gtacaacgtc tattcgcatc tcgtctatgc
     7501 gacgaaggcg gcgaacgtgc gcacggtagt tatcgaaggg cgcgtcgtga tgcgcgaccg
     7561 ccgcttgctg acgctcgacg aagaggccat caagcgcgac gcgcgtgctt accgcgtgcg
     7621 gatttcgcaa agtcttgccc cggcgtcggt gagtccggcg ggcgcaaagc gcgacgagag
     7681 cgatacgagc gggacgcgaa agcgcaaacc ggcgacgcgc ccggcgcgga aaaaaccggc
     7741 gcggcggagt tgatcgattc gcattgacgg gcgataatat tcgcgctgat caaagatcac
     7801 ccttcaagcg agcgtcaacg ctcgctttca catttatgga gttgtcatag tgaaggaaag
     7861 tccccttatt cgttcgacac gaacgcttgc caaagtcact ttcagtctgc tcttaattct
     7921 cgccttcgcg ccgggtcttc gtgcgcagag ttcgaacgat ctcgacgcag agggtatcca
     7981 acggcgcatc acgcgcgcgc gtgcgctggc ggcggcgggc aatctccctg cggcgcgcag
     8041 cgaactcgaa gcattgcagc gcacggcggg cgtcgatcct tccgtgcgcg agatcacgct
     8101 cgtgctgctc gccggaattt atatcgacca ggccgactac acttatgcgg agaacctctt
     8161 gaacgcggcc tttggcgcgc gaacgacggg cggcgagagt gcggcgcgtg cttacttcgt
     8221 gctggcgggt caggttttgg gcggcgtgcg cgcgcgcctc gaacgttatc gcgtgttcgg
     8281 cttgaacgtg gcggacgagg cgttgcccgc agaggcttcg ggcgacgtgg atcgcctgcg
     8341 gacgctcatc gaaaaagttt tcgcacaggg aaggcaactg cgcgacgaga acgcgcgcaa
     8401 catggatgcg acggctttac tggaagaggc tgcgggcgtg cgcgtgcgtc tcgcgcgcag
     8461 ggaagacgag cgcgcactct ggcagcgcga gttgacggat gcgcgccagc gtctcatcgg
     8521 cacaaccgca cgcgcggcct ctaacggcac aaatgctccg aacgcgcgca cgcaggggag
     8581 ttcgattttg gcgggttcga gccaacccgc gaacgccgcg cagccttcgc ccacccctgc
     8641 gaacgctgcg ccctcaactt cgacgaacgc gcccaacgcc gcacagaata ctgctgcgaa
     8701 cacgcagcgc gggagttcga cggcgacgac aactgcgccc ccgaacgccc gcccctccgc
     8761 gacacctgcg ggcggcaacg cgaccgaagg caggccgctc gaagtcggct cgctggtgcc
     8821 gagagcggcg cagacggttg cgcccgccta tccgccgacg gcgcgtagcg cgcgggtcgc
     8881 gggcaaggtc acggtctttt tgatggtgga tgagaagggc gcggtcgcag cggtcgaacg
     8941 cacggacgga ccggaactgt tgcgtcgtgc ggcggaagag gccgcgcgcc gctggaagtt
     9001 tcgcccgaca atcgttaacg gtcagcccgt gcgcgtctca ggtttcatca acttcaattt
     9061 cacactttag acggagcgca cgcacccgtc acgatgcttc gcaggataaa ccggagtggc
     9121 agaggattcg tcttcagccg ctccggtttt tgtttgccgt cgagtcgaag cagagacatg
     9181 cggcgcagag acggggagag tcgggacatg atgtatgcgg gatgatagcg tgcagcctgt
     9241 gagccgccgc gtgcttgcct gctgttccga ttcatcattc cgcgttccgc attcgccatc
     9301 ctcctctgta ctctacgtgt ctccgcattg agtctgcgag cctgccttta aaaaaaatct
     9361 tgcccgcctt caaagccttc gtttaagatg cacgcacccc tggcacccct tgttcagttg
     9421 tcgttaactt gtcgttaaaa aacgctgcaa cttcgctgca cgtagtgctg tgcgtccatc
     9481 cgcgacgttg aagcatgtcc cgcacggcgc accgcgctcc tcagtccgcc gccgcgaaac
     9541 gaacacaagg aaaggaagaa aggccgatga gatatcccac acacctcatg cagggcttag
     9601 tgtccctgct gccgaaacga cagacgaact tcacacccaa ccaacgaaaa aacggatcaa
     9661 accgcacggc cggagcattc atctgagccg ccgtctgcct gacgttg
//
LOCUS       DASWNS010000295         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_505023, whole genome shotgun sequence.
ACCESSION   DASWNS010000295 DASWNS010000000
VERSION     DASWNS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505023"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VGB76_10445"
     CDS             complement(<1..200)
                     /locus_tag="VGB76_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099357.1"
                     /translation="MAQDSTIDTLDENRAGSLYDRIARLYDLTFKFNGYGRSLERYFR
                     EHPLPLEDNAQILDAGCGTGLLT"
     gene            401..1213
                     /locus_tag="VGB76_10450"
     CDS             401..1213
                     /locus_tag="VGB76_10450"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEY0099358.1"
                     /translation="MFETEQDVLDWYEAQPRAITREFVESLNWQEIREHPLHPAFKDV
                     IVYMRDVESFTEIYYKELLRTPTGKNPVIRKFMDRWSGEENEHADLLNRFLNEAGYPT
                     SETWQSEARAAIPFRYTFENNLYPLITNCFGKYFSGAHMVWGAINEMTTLQGYRRLWK
                     LAGHPVLEQLLRAIAREESVHSHFYWSIARLKLERSGYSRALARIIIGKFWSPVGQGT
                     KPQAETDFVIGTLFKGRDGVEFFNQNVNQRLQSLPGFTGLENVSQRIARIAL"
     gene            1232..1945
                     /locus_tag="VGB76_10455"
     CDS             1232..1945
                     /locus_tag="VGB76_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0099359.1"
                     /translation="MRRKLPIIIIVGVLLVALAGGALLMRPSTPQVNIAQLPPTITTP
                     NPQATAPSAPRPAASNIAPITHLRGRADAPVLIEEYGDFQCPPCGFFHPILKRLEGEY
                     PTQLRVTFHHFPLRNMHKHAEQAARAAEAAAVQGKFWQMHDMLFEKQKEWNEAANVRP
                     VFLGYARTLGLDVQKFSQDMDSVMVGSRVMNDENGGTTRGVTGTPTVFLNGREVPYET
                     AIDYNRLRAVIESELAGRK"
     gene            1942..>2129
                     /locus_tag="VGB76_10460"
     CDS             1942..>2129
                     /locus_tag="VGB76_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099360.1"
                     /translation="MISNGTVKDAGNAASEGEAASPRLSRLYALAAVLALAGLADAVY
                     LTVEHLTGRSVRCTVTSG"
BASE COUNT          467 a          622 c          625 g          415 t
ORIGIN      
        1 gtcaagagtc ctgtgccgca cccggcatcg agaatttgcg cgttgtcttc gagaggcagc
       61 gggtgctcgc ggaaatagcg ttcgagcgaa cgcccgtagc cgttgaactt gaaggtcaga
      121 tcgtagaggc gcgcgatacg gtcgtagagc gagcctgcgc ggttctcgtc gagtgtgtcg
      181 atggttgagt cttgtgccat gcgctcgctc cgtccctctt tcccgggacg gtaaaggccg
      241 cctgtgatcc tatgcgcgac taaaacttca cacgcccatc gaacgtaaca ccgggcggaa
      301 gccaaacgtc aagggaaatc caaagggtag tagttatgcg taaattaaaa gaacgtgaca
      361 cttatacaaa tactctacga aatccacaag gagactcttc gtgtttgaga cagaacagga
      421 tgtgcttgat tggtacgagg cgcaaccgcg cgccataacc agagaatttg tcgagtcact
      481 gaactggcaa gagatccgtg agcacccgct ccatcccgcc ttcaaagacg tgatcgtcta
      541 tatgcgcgac gtggagagtt ttacagagat ttactacaaa gagttgttgc gcacgccgac
      601 gggcaagaat cctgtcatca gaaagtttat ggatcgttgg agcggcgaag agaacgagca
      661 cgccgacctg ctcaaccgct ttttgaacga ggccggatac ccgacgagcg aaacatggca
      721 gtcggaagcg cgtgcggcga tccccttccg ctacaccttc gaaaacaacc tctatcccct
      781 aatcaccaac tgtttcggca aatacttttc cggcgcgcac atggtctggg gcgcgatcaa
      841 cgagatgacg acgctgcaag gctaccgacg cctctggaaa ctggccgggc atcccgtgct
      901 cgaacaactg ctgcgcgcga ttgcgcgcga agagtcggtc cattcacatt tttactggag
      961 catcgcgcgt ttgaaactgg aacgctccgg ctattcgcgt gcgctcgcgc gcatcatcat
     1021 cgggaagttc tggtcgcccg tggggcaggg cacgaagccg caggccgaaa ccgatttcgt
     1081 catcgggacg ctcttcaaag gcagagacgg cgtagagttt ttcaaccaaa atgtcaacca
     1141 gcgtctgcaa tcgctgcccg gcttcacggg gctggaaaat gtctcgcagc gaattgcgcg
     1201 gatcgcgctc taaacgttat gctctgcgtt tatgagacga aaacttccta tcatcatcat
     1261 cgtcggggtg ctcctcgtcg ccctcgcggg cggcgcgctt ttgatgcgcc cgtcaacgcc
     1321 gcaagtaaac atcgcacagt taccgcccac gatcacgact cctaacccgc aggcgaccgc
     1381 ccctagcgcg ccgcgccccg ccgcgtcgaa catcgcaccc atcacgcact tgcgcggacg
     1441 cgccgacgcg cccgtgctga ttgaagagta cggtgatttt cagtgtccgc cgtgcggctt
     1501 ttttcatccg atcctgaagc gtctggaagg tgagtatccg acgcagttgc gcgtgacgtt
     1561 tcatcatttc ccgctcagaa atatgcacaa gcatgccgaa caagccgcgc gcgccgcaga
     1621 ggccgccgca gtgcagggca agttctggca gatgcacgat atgcttttcg aaaagcagaa
     1681 ggaatggaac gaagcggcga acgtgcgccc cgtgttcctc ggctacgcgc gcacgctcgg
     1741 actcgacgtg cagaaatttt cgcaggatat ggacagcgtg atggtcggca gtcgcgtgat
     1801 gaatgatgag aacgggggca cgacgcgcgg cgtgaccggg acgccgaccg tgtttctcaa
     1861 cgggcgcgaa gtgccgtatg aaacggcgat tgattacaac cggctccgcg ccgtcatcga
     1921 aagcgaactc gccggtagaa agtgatctca aacggcacag ttaaagacgc gggtaatgcc
     1981 gcgagcgaag gcgaagcggc gtcgccgcgc ctctcgcgcc tgtacgcgct ggcggcggtg
     2041 ctcgcgctcg cggggctggc cgatgccgtc tatctcaccg tcgaacatct gacggggcgc
     2101 agcgttcgtt gcaccgtcac ctccggttg
//
LOCUS       DASWNS010000296         6056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_480486, whole genome shotgun sequence.
ACCESSION   DASWNS010000296 DASWNS010000000
VERSION     DASWNS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480486"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..2002)
                     /locus_tag="VGB76_10465"
     CDS             complement(209..2002)
                     /locus_tag="VGB76_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEY0099361.1"
                     /translation="MDSSNYHLINTPAELDASIAELSQHPVVGFDTETTSLDPYSGRM
                     RLMQFSAPDGTAHIIDLDRFRNGDGNGSPAQNETLAPLRELLAAPRPVKVAHNAKFDA
                     KWTKHQLGVELGGIFDTLLASQIVSAGESEDRHSLAAVAARYLDETVDKSEQLSDWSG
                     ELSEAQLEYAARDAALMLPLRERLIELLKRDELVRVAQLEFECVMPVACLELAGIYLD
                     AERWREQIAIVKTKRAQLAAELSAMLAVGTVQGSLFENARADINLDSHTQLTAALKRL
                     GVPLPDSTRNWKLQPLAAEWPVVAKLLDYRTMQKSLTSYGENILGEINKTTGRIHANF
                     HQIGAPTGRFACTNPNVQQVPHAPEYRRCFRAPDGRKLVIADYSQIELRILAHVTEDK
                     GFIEAFNSGADLHRVTAAQVFNTPLEEVTKEQRDFAKRLNFGVVYGIGAQRFSMMTGI
                     PLTEAESILARYFQTYRGLDTWLREAARKAVRERTARTMSGRLARFNFDQEDRQAASL
                     AQRNGKNMPIQGASADILKRALRLLHDKLKETSARVVNIVHDEIVVECDGDECQEIAR
                     TVEEAMCAAGEEYVTRVPVKVETEIADEWVK"
     gene            complement(2125..3528)
                     /locus_tag="VGB76_10470"
     CDS             complement(2125..3528)
                     /locus_tag="VGB76_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0099362.1"
                     /translation="MAETDTDPADEFDASLSPVALDERTPGLFRRYRRLPRNVLVISL
                     VSFLNDASSEIIYPLLPVFLSATLGASPAIIGLIEGGAESVSSFLKLFAGYFSDRSGR
                     RKAPVVFGYALASIARPLLGFATSWPQVFAVRFVDRVGKGIRSAPRDAMIADSAAPTE
                     RGLAFGFHRAMDHLGAVAGPLLAYVLVAYIAIDDNAITPGEYRLIFLAASVPALAAVL
                     VAAFVIRETRRKGSAEARRGQAAPDGENARRARSNFEPERAPAANLTTPPKPPRFSLS
                     GFDRNFKRFLIIIALFTLSNSSDAFLLLRARDVGIATANLPLLWAALHAVKVLSSLVG
                     GDLSDRLGRKTLIVTGWLFYAAVYLGFAFVSTVAGVLTLFLIYGIYFGLVEGAEKALV
                     ADLVPADKRGTAYGLYNLAFSITVWPASLLMGALWSWRGAGVAFIASACIGTLAALLL
                     AFTIRSGAETLDTSPAR"
     gene            complement(3547..3768)
                     /locus_tag="VGB76_10475"
     CDS             complement(3547..3768)
                     /locus_tag="VGB76_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099363.1"
                     /translation="MTIGIIGIALIIALVVLFLVWRTVKVFIKLALIGLIALLVVAAL
                     VWWNFSGGETNPTPNRNRPATNMRRNANR"
     gene            complement(3868..4293)
                     /gene="queF"
                     /locus_tag="VGB76_10480"
     CDS             complement(3868..4293)
                     /gene="queF"
                     /locus_tag="VGB76_10480"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY0099364.1"
                     /translation="MSVVETAIPSVNQQGLDIRPTPREEMNLYPLDTFPYEFVGREIS
                     VSFEMPEFTAICPFSDFPDFANIRLVYVPHERCVELKSLKLYINSFRNVKIFHEHVVN
                     VILEDFVRACAPLSVELEGDFNIRGNIKTVVRASYKKND"
     gene            complement(4437..5060)
                     /locus_tag="VGB76_10485"
     CDS             complement(4437..5060)
                     /locus_tag="VGB76_10485"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY0099365.1"
                     /translation="MGKGKKLGFMLSATLMCVAMLAATATAQNTNTGSTTRTGNGNNG
                     NNRGNGGTASVSSTDRKFAMQAAMGGMAEVEVSRLALTKASSDAVKQYAQKMIDDHTA
                     ANTELMQIASTKGITLPTAPDAKHRAMMARMERLSGEAFDREYVMMAGHKDHQKMEKL
                     FRDESTRGRDADLKAFAAKTLPVVQQHLQLAREMHDRMMPGMNHNNR"
     gene            complement(5168..5650)
                     /locus_tag="VGB76_10490"
     CDS             complement(5168..5650)
                     /locus_tag="VGB76_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEY0099366.1"
                     /translation="MADNDKAISVLNSLIETCKDGQNGFQTAADGIKRSELKSLFYQY
                     SQQRAQFAGELQEEVRRLGGDPEQAGSLAATLHRGWMNIKSAVTGEDENAVLAEAERG
                     EDAAVASYKDALADQNLPADVRSVVERQYAQVQEAHDRVRNLERATGTTGGSTNTASV
                     "
     gene            complement(5726..>6056)
                     /locus_tag="VGB76_10495"
     CDS             complement(5726..>6056)
                     /locus_tag="VGB76_10495"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY0099367.1"
                     /translation="PYIPSLGASGAISGVLGGYLLLHPTRGVHVIVFSFLVRTVPAFV
                     AIGLWFVFQLINGLGMLGQGSQEGGGVAYAAHIGGFIAGLLLVKFFDSGAKSDPTQMW
                     ASRRAGY"
BASE COUNT         1085 a         1762 c         2016 g         1193 t
ORIGIN      
        1 tgtgagatac cggtgggctg actcgaccgg gtgtgagtta agcgaacaga ggcgcgagga
       61 ctggcgaacc gaacaacgtt ctacgtggat gatacaacgc aaaaaggaaa ggatgaagga
      121 tgcgggatga gggatgaatc agaaagacgc tcaactgagt tgcatgctta tcttactcat
      181 ccctcatcct tcatccctcc tccctttgtt acttcaccca ctcgtcggca atttccgtct
      241 ccactttgac ggggacgcgc gtgacgtatt cctcgcccgc cgcgcacatg gcctcttcca
      301 ccgtgcgcgc gatctcctgg cactcgtcgc cgtcgcactc gacgacgatc tcgtcgtgca
      361 cgatgttgac gacgcgcgcc gaagtctctt tcagtttgtc gtgcagcagg cgcagggcgc
      421 gcttgaggat gtctgcggac gcgccctgaa tgggcatgtt cttgccgttg cgctgtgcga
      481 gcgaggcggc ctgccggtct tcctgatcga agttgaagcg ggcgaggcgg cctgacatgg
      541 tgcgcgccgt gcgctcacga acggccttgc gcgccgcctc gcggagccac gtgtcgaggc
      601 cgcgataggt ctggaagtag cgcgcgagga tgctctcggc ctcggtgaga gggatgcccg
      661 tcatcatgga gaaacgctgc gcgccgatgc cgtagaccac gccgaagttg agacgcttgg
      721 cgaagtcgcg ctgctccttc gtgacctctt cgagcggcgt gttgaagacc tgcgcggcgg
      781 tgacgcggtg gaggtctgcg cccgaattga acgcttcgat aaagcccttg tcttcggtga
      841 cgtgcgcgag gatgcgcagt tcgatctgcg aatagtctgc gatgacgagt ttgcgcccgt
      901 ccggcgcgcg gaagcaacgc cggtactccg gcgcgtgcgg cacctgttgc acgttcggat
      961 tggtgcaggc gaagcgtccg gtgggcgcgc cgatctggtg gaagttggcg tggatgcgcc
     1021 cggtcgtttt gttgatctcg ccgaggatgt tttcgccgta gctcgtgagc gatttttgca
     1081 tcgtgcggta gtcgagcaat ttggcgacga ccggccattc ggcggcgagc ggttggagtt
     1141 tccagttgcg cgtggaatcg ggaagcggca cgccgaggcg tttgagcgcg gcggtgagtt
     1201 gcgtgtgcga atcgagattg atgtcggcgc gtgcgttctc gaagagcgag ccttgcacgg
     1261 tgccgacggc gagcatcgcg ctcagttcgg cggcgagttg agcgcgcttt gttttgacga
     1321 tggcgatctg ctcgcgccag cgttcggcgt cgaggtagat ccccgcgagt tcgagacagg
     1381 cgacgggcat cacgcactcg aactccaact gcgcgacgcg cacgagttcg tcgcgcttca
     1441 agagttcgat gagacgctcg cgcaagggca gcatgagcgc ggcgtcgcgc gcggcgtatt
     1501 cgagttgggc ttcggagagt tcgccgctcc aatcgctgag ttgttccgac ttgtctaccg
     1561 tctcgtcgag gtagcgcgcg gcgacggcgg cgagcgagtg gcggtcttcg gattcgcccg
     1621 ccgagacgat ctggctggcg agcagcgtgt cgaagatgcc gccgagttcg acgccgagtt
     1681 gatgcttcgt ccacttggca tcgaacttcg cgttgtgcgc cactttcacg gggcgcggcg
     1741 cggcgagcaa ttcacgaagc ggcgcgagcg tctcgttttg cgcggggctg ccgttgccgt
     1801 cgccattcct gaagcggtcg agatcgatga tgtgcgccgt gccgtcgggc gcggagaact
     1861 gcatgagacg catgcgcccg ctgtaagggt cgagcgaggt ggtttccgtg tcgaagccga
     1921 cgaccggatg ctgcgacagt tcggcgattg acgcgtcgag ttcagccgga gtgttgatca
     1981 gatgatagtt tgaagagtcc atgagtgatt gatgttaccg gaaaaaagtt tagcgggcga
     2041 attgcccggc gtgaacgcct tactggcagc gcgaaaacgc gcggcactat agcatcattt
     2101 tgcgtccgcg cccgacgcgt aagttcaacg cgccggagaa gtgtctaaag tttccgcgcc
     2161 ggaacggatc gtgaaggcga gcaggagcgc ggcgagcgtg ccgatgcacg cgcttgcgat
     2221 aaaggcgacg cccgcgccgc gccaactcca cagcgcgccc atcaacagcg acgcaggcca
     2281 gaccgtgatg ctgaaagcga gattatacag gccgtaggcc gtgccgcgtt tgtctgcggg
     2341 cacaaggtcg gccacgagcg ccttctccgc gccttcgacg aggccgaaat agatgccgta
     2401 gatgagaaac agtgtcaaca cgcccgccac cgtcgagacg aaggcaaagc ccagatagac
     2461 cgccgcgtaa aagagccagc ccgtcacgat caaagtcttg cgccccagac gatccgacag
     2521 gtcgccgccg acgagcgaac tgagcacctt gacggcgtgc agcgcagccc agagcagcgg
     2581 gaggttggcg gtggcgatgc ccacgtcgcg cgcacgcagc agcagaaagg cgtcggagga
     2641 gttagataac gtgaagagcg cgatgatgat gagaaagcgt ttgaagtttc ggtcgaagcc
     2701 gctgagcgaa aaacgcgggg gcttcggcgg cgtggtgagg tttgcagcgg gcgcgcgctc
     2761 aggttcaaag ttcgagcgtg cgcggcgggc gttttcaccg tcaggcgcgg cctgcccgcg
     2821 tcgagcctcc gcgctgccct tccggcgcgt ctcgcgaatc acgaaggcgg cgacgagcac
     2881 ggcggcgagc gcggggacgg aggcggcgag gaagatcagg cgatactcgc cgggcgtgat
     2941 ggcgttgtcg tcaatggcga tgtaggcgac gagcacgtag gcgaggagtg gtccggcgac
     3001 cgcgcccaga tgatccatcg cgcggtggaa accgaaggcg aggccgcgct cggtgggcgc
     3061 agccgagtcg gcgatcatcg cgtcgcgcgg cgcgctgcgg atgcccttgc ccacgcggtc
     3121 tacgaagcgc acggcgaaga cctgcggcca actcgtcgca aagcctaaga gcgggcgcgc
     3181 gatgctcgcg agcgcgtagc cgaagacgac gggggctttg cgtcgcccgc tgcggtcgct
     3241 gaagtatccg gcgaagagtt tcaggaaact ggagacggat tccgcgccgc cttcgatgag
     3301 tccgatgatg gcgggcgatg cgccgagcgt ggcggagagg aagacgggca gcagcgggta
     3361 gatgatctcg ctcgaagcgt cgttcaggaa actgacgagg ctgatgacga gaacgttgcg
     3421 cgggaggcgt cggtagcgtc gaaagaggcc gggcgtgcgt tcgtccaacg cgacgggact
     3481 gagcgaggcg tcgaactcgt ctgcggggtc ggtatcagtc tcggccatta aagggattgc
     3541 agagaatcag cggttggcgt tgcggcgcat gttcgtggcc gggcgattcc tgttcggcgt
     3601 cggattcgtc tcgccgccgg agaagttcca ccacaccagc gcggcgacga ccagcaaggc
     3661 gatcaggccg atgagcgcga gcttgataaa caccttcacc gttcgccaca cgaggaacag
     3721 cacgacgagc gcgatgataa gagcgatacc gatgattccg atggtcataa aggaagctgt
     3781 tagcgtttcg ctgttggctg ccggttccgg gcgattggtc gtcagccttt agcgaggcaa
     3841 tcaattcgca tggctcagag ccgggagcta atcgttcttc ttgtaacttg cgcgcacgac
     3901 cgtcttgatg ttgccgcgaa tgttgaagtc gccttcgagt tcgacgctca agggggcgca
     3961 ggcgcgcacg aagtcttcga ggatgacgtt gacgacgtgc tcgtggaaaa tcttcacgtt
     4021 gcgaaacgag ttgatataga gcttgaggct tttcagttcg acgcaacgct cgtgcggcac
     4081 gtagacgagc cggatgttgg caaagtcggg aaaatcggag aacgggcaga tggccgtaaa
     4141 ctccggcatc tcaaaactga cgctgatctc gcgcccgacg aactcataag ggaacgtgtc
     4201 gagcgggtac aggttcatct cctcgcgcgg cgtcgggcgg atgtccaagc cctgttggtt
     4261 gacggaagga attgcggttt cgacaacaga catgcgttta aatactcctt gaatctacga
     4321 gccgcccgat gttacatgag ccgcgcggga gaaagcaaaa ggcgaaggtc ggagggcgct
     4381 ctgccagccg cccctcgcct tcgcctcgcc tcctgccgtc gagctttcgc ccgcgtttac
     4441 cggttgttgt ggttcatgcc cggcatcatc cggtcgtgca tctcgcgcgc caactgcaag
     4501 tgctgctgca cgacggggag cgtcttggcg gcaaacgctt tcaagtcggc gtcgcgtccg
     4561 cgcgtgctct cgtcgcggaa caacttctcc atcttctgat ggtctttgtg tcccgccatc
     4621 atgacgtact cgcggtcgaa agcctcgccg gaaagtctct ccatgcgagc catcatcgcg
     4681 cgatgcttcg cgtcgggcgc ggtgggcagg gtgatgccct tcgtcgaagc gatctgcatc
     4741 aactcggtgt tggccgccgt gtgatcgtcg atcatcttct gcgcatactg cttgacagca
     4801 tcgctcgacg ctttcgtcag agcgagccgc gacacttcca cttcggccat gccgcccatc
     4861 gccgcctgca tcgcaaactt cctgtccgtc gaactgacgg acgccgtacc gccgttgccc
     4921 cggttgttgc cgttgttgcc gttgcctgtc cgcgtggtcg agccggtgtt cgtattttgc
     4981 gccgtggccg tcgcggccag catagcgaca cacatcaacg ttgcgctcag catgaagccg
     5041 agctttttac cttttcccat gttcgttctc cctctggttg tagtctcttt gtgagttgtt
     5101 tccggttagt tgacgaaaga cgtgggagct tttttttgac cggctcccac gcccgaatga
     5161 ttaactttta gacgctcgcc gtgttggtgc tgccccccgt cgtgcccgtc gcgcgttcga
     5221 ggttgcggac gcggtcgtgt gcctcctgta cctgcgcgta ctggcgttcg acgactgagc
     5281 gaacgtccgc aggcaaattc tggtcggcga gcgcgtcctt gtaacttgcg accgcagcat
     5341 cttcgccgcg ttcggcttcg gcgagcacgg cgttctcatc ttcgcccgtc acggcggact
     5401 tgatgttcat ccaaccgcga tgcagcgtgg cggcgaggct gcccgcctgt tccgggtcgc
     5461 cgccgagcct tcgaacttcc tcctgcaatt ctcccgcgaa ctgcgcgcgc tgctgcgaat
     5521 attgatagaa gagcgacttg agttcgcttc gcttgatacc gtcggcagcg gtctggaaac
     5581 cgttctgccc gtccttgcag gtttcgatca agctattgag tacggagatc gctttatcgt
     5641 tatcagccat ttcatttctc ccttttcgaa gtttcgcggc gcggcgtcaa tcgctgaacg
     5701 gacgccgcgc gccgcttcgg attcactaat acccggcgcg cctgctcgcc cacatctgcg
     5761 tggggtcgct cttcgcgccg gagtcgaaaa atttgacgag caggagtccg gcgatgaagc
     5821 cgccgatgtg cgcggcgtag gccacgccgc cgccctcctg tgaaccctgc ccgagcatgc
     5881 cgaggccgtt gatcaactgg aagacgaacc agaggccgat ggcgacgaag gccggaacgg
     5941 tgcgtaccag aaaactgaac acgatgacgt ggacgccgcg cgtcgggtgc agcagcaggt
     6001 agccgccgag cacgcccgag atcgcgcccg atgcgccgag actcggaatg taaggg
//
LOCUS       DASWNS010000297        20857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_63092, whole genome shotgun sequence.
ACCESSION   DASWNS010000297 DASWNS010000000
VERSION     DASWNS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20857
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63092"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..484)
                     /locus_tag="VGB76_10500"
     CDS             complement(59..484)
                     /locus_tag="VGB76_10500"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY0099368.1"
                     /translation="MKRNASAEWRGGLKDGKGTISTNSGVLSETQYSFSTRFEDGIGT
                     NPEELIAAAHAGCFSMALSGQLGKADLTAESIRTTATVTLDKVEGNFTITEVHLQVAA
                     RVPGASQEQFDTAANNAKAGCPVSRVLNAKITLDAQLEA"
     gene            complement(527..1564)
                     /locus_tag="VGB76_10505"
     CDS             complement(527..1564)
                     /locus_tag="VGB76_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0099369.1"
                     /translation="MKFKLQKFLVLLCLVALVCSSCATKANDADDRSRVRVGIVFDIG
                     GKDDRSFNAAAFEGVQRAERELGIVLRDVEPGNPTSIEPAMRAFAERGYDLVIGVGFA
                     QAPIMEIVARDYPHINFAIVDGVSQLPNVDSLVFKEHQGSYLVGMIAARTTKTGVLGF
                     VGGMDIPLIHRFAKGYEEGAQAVNPSVRVVRNFVGVTDSAWNNPGKGKELALSQIGKG
                     ADVIFTAAGNSGLGAFDAVEQAGKGADGRATHFVIGVDSNQNMVKPGYVLTSMVKRVD
                     NAVYDIVNDVKNKRFTGGIKVFGLDKDGVAFSLDEFNRPLIDPAVLQEVETARQKIIK
                     GEIQVTDAMAK"
     gene            complement(1561..2886)
                     /locus_tag="VGB76_10510"
     CDS             complement(1561..2886)
                     /locus_tag="VGB76_10510"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEY0099370.1"
                     /translation="MRPQDVIRRKREGGALSREEIEFFVEGVTSGAFADYQAAALLMA
                     IFCRGMDETEQRTITEAMLHSGEVLDFSDIPLPKADKHSTGGVGDKTSLVIAPLAAAC
                     GLAVPMISGRGLGHTGGTLDKLESIPGYQVKLSLAEFRRVLKLCGFAMMGQTAEIAPA
                     DRKLYALRDATATVETIPLIVASIMSKKLAAGLDALALDVKTGTGAFMREEARARELA
                     HALVSTGNACGVRTEALLTDMNQPLGRAVGHALEVHECIEILRGQTPAGARPVLDLSL
                     ELTARMCALANVEPTIEAARVRVGRALESGAALECFRRNVELQGGDARVCDDPSLLLQ
                     TSTLRAVKVESPRAGFVTQVETAEIGFAVSAIGGGRTRVEDEIDFQVGFIAEAVHGDE
                     LRAGDALGTLYCRDDAQAAGAGARIQAAYAIGVEPPDAPLMLVKEVITA"
     gene            complement(3134..3610)
                     /gene="ispF"
                     /locus_tag="VGB76_10515"
     CDS             complement(3134..3610)
                     /gene="ispF"
                     /locus_tag="VGB76_10515"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY0099371.1"
                     /translation="MFRIGIGHDTHRLADGRPLILGGVLVESERGAEGHSDADALAHA
                     ITDAILGALCEGDIGVHFPDNDPRWKDADSLQLLARVVWLASQSGFKVVNVDANVSLE
                     RPRLRPHIPEMRERLAQVLSIEANCVSVKAKTGEGLDAVGEGRAIVAQAVVLLGRI"
     gene            complement(3693..4376)
                     /gene="ispD"
                     /locus_tag="VGB76_10520"
     CDS             complement(3693..4376)
                     /gene="ispD"
                     /locus_tag="VGB76_10520"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY0099372.1"
                     /translation="MNVAIIVAAGRGTRAGGEGLKQFRALAGVPLLLHTLRRFEECDA
                     VDEIVLVAPFAEANFHLSASPFKLTKLARVVEGGTTRTASVGRGLQTLNETRTRIVAV
                     HDGVRPFVTPAEIERTIRAAGEMGAAVLCAPVTDTIKVVEGERIAHTLPRETLRRALT
                     PQCFRYELLSRAHERARAGEIEATDDSALVELCGAQVAFIEGDARNIKITRPEDFALA
                     EILLKESGG"
     gene            complement(4543..5676)
                     /locus_tag="VGB76_10525"
     CDS             complement(4543..5676)
                     /locus_tag="VGB76_10525"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0099373.1"
                     /translation="MKTDLILIRLVFTALLVAAGYVLHPIRNQPLLSAGVAAVIAFAI
                     IMFERRIQRASLKTLIGAAVGSILGIVGAYLIGSLISTQHSDAVAPGTKTFLTLALTF
                     LMAYIGLVVGAAKGDYLDLSALGGIFSDKTVRRDFKILDTSVIIDGRIADIAEAGFLS
                     GTIVIPQFILRELQQVADSPDSNKRQRGRRGLDMLNRLQTNNTLDIQVVDTDFPAVRE
                     VDLKLLELSKQLDAVVVTNDFNLNKVAQLHGVSILNINELANALKPVVLPGELMRVFI
                     LKEGKEYNQGVAYLDDGTMVVVDNARKMIGKNADISVTSVLQTTAGKMIFGRLSEESH
                     ENGASIHNTDTRGINGNNTPRRPYRDPRPHPTLAARSIEPEEN"
     gene            complement(5873..7246)
                     /gene="radA"
                     /locus_tag="VGB76_10530"
     CDS             complement(5873..7246)
                     /gene="radA"
                     /locus_tag="VGB76_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY0099374.1"
                     /translation="MAKGAGTVYVCQNCGKQSAKTLGKCPDCGEWNSFVEERVRATPK
                     GAAGRGAFRTREVKPVAYAEIESQDDVRVSSGITEFDRVLGGGIVPGSLVLIGGDPGI
                     GKSTLLLEVADKLSAGGARVLYVSGEESERQIKMRGERLGVKAENLYLLPETNLEAIF
                     DEIKRDEPGAVIVDSIQTVFSSGIESAPGSVSQVREVAGQFLRFAKDHGVPVFLIGHV
                     TKEGSIAGPKALEHIVDTVLYFEGERHHNHRIVRATKNRFGAANELGVFEMTGTGLKP
                     VANPSEMFLQERPLHAAGSVVTACMEGTRPMLVEVQALVSGSQYNYPLRKAQGVAEGR
                     IALLIAVLEKRVSGYRLREEDVFINVAGGLDIDEPAADLGVIAAIASGFKSKPIEAQT
                     AVFGEVGLTGEVRGAMQAEGRAREAQTLGFKKLIVPASNAEGLQRLLGLRVVGVRSVE
                     EALDELF"
     gene            7396..8457
                     /locus_tag="VGB76_10535"
     CDS             7396..8457
                     /locus_tag="VGB76_10535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0099375.1"
                     /translation="MTEAQAGGLGCRICGNEAANALHHAREMFYGTRHPFAYVECAAC
                     GTIQIQQVPDLRPYYAQNYYSFAHAQADAQPAALGIKRNLAARVGAFVRRTAGAYYGQ
                     RRARLGQLRHPLGRSLSALLPRLVAGFPEYLLDTRVNLNLTPRSAILDFGSGAGSTLL
                     TLSLFGFRDLTGVDPFVEADIEYGDGVRVLKTELSGLSRQFDLILANHSVEHVADPRA
                     TLVEIRRVLKPGRFAVIRIPVVAHAWERYGVNWVQLDPPRHLFLFNVHTFAGLATEAG
                     FALEELRHDSTAFQFWGSEQYARDIPLTDERSYLLNPAASIFEAGQLAAFAAQAEELN
                     RRGEGDQAVFYLRKPVTGD"
     gene            complement(8514..9287)
                     /locus_tag="VGB76_10540"
     CDS             complement(8514..9287)
                     /locus_tag="VGB76_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099376.1"
                     /translation="MPDLAHIAAKYDTDKAVHAEYLRNYEHYFGELREREVLLFELGI
                     KKGGSLYLWRDYFERGRIVGLDVNPVQLEDASGRIRVYEGMQQDTELLDRIARENAPA
                     GFDIIIDDCAHNGVLARASFWHLFEHHLKSGGVYVIEDWGTGYWDGWLDGVAYRRGRK
                     SLNPVLYRSTRAVSRLQQNQVAGRVPLVGKLLSKMKAAVLGAQYHSHDYGMVGFVKEL
                     VDELGMADITHPQMGTGAPRKSKFREMRLSPSHLFIVKA"
     gene            complement(9372..9680)
                     /locus_tag="VGB76_10545"
                     /pseudo
     CDS             complement(9372..9680)
                     /locus_tag="VGB76_10545"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
     gene            complement(9935..10252)
                     /locus_tag="VGB76_10550"
     CDS             complement(9935..10252)
                     /locus_tag="VGB76_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin-associated ferritin-like protein"
                     /protein_id="HEY0099377.1"
                     /translation="MATSAEWHESYEQMSELTLNFSRAVKSVQEELEAVDWYNQRAEV
                     TKDDQLRRILEHNRDEEIEHAMMALEYLRRISPVFDKHMRTYLFTEGDILEIEERAED
                     HPK"
     gene            10467..11279
                     /locus_tag="VGB76_10555"
     CDS             10467..11279
                     /locus_tag="VGB76_10555"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0099378.1"
                     /translation="MFTHLIESGSHKKEFARKGRFFLGTLGFYGLLLMVAGVASVYAY
                     NEQLGNQDLEVTLVTPVRVTEIERNPDVVRPARPSHTSGSANTGGLFRREIIASTDNP
                     LIPKGISTHPAPPPSPPGAKQGTRDIGDGIVIGPPDNGSDDTGTTTSRVVVPVATDPD
                     PLPPPRATPTPAKQVGQVRLPSVVISSKIITKPAPVYPAIARQTRVQGTVTVEILINE
                     QGRVISAQATNGHPLLRAAAQSSAFQAKFSPTSISGQPVKVSGVITYNFILQ"
     gene            complement(11414..12619)
                     /gene="alr"
                     /locus_tag="VGB76_10560"
     CDS             complement(11414..12619)
                     /gene="alr"
                     /locus_tag="VGB76_10560"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0099379.1"
                     /translation="MTEHDLTQNTGADGDDEALAARPTWAEIDLDALAANFHEVRRRV
                     GGEVKVMAVVKADAYGHGAVRCARRLAAEGAEWFAVALPEEGIELRRAGIVQPILCLE
                     GFWEGQARACLEHKLVPVVYRLDMAEAYESAAREACVVADVHVKIDTGVGRLGVRYGG
                     AAKEFARALKNFGHLRVDGLMTHFASADDAARDSLTAEQSECFEAARDSFRAEGHAPT
                     FFHMANSAATFAHPRARGNMVRPGGVLYGLWRDVLQTQREPPELRPVLAMRSRIILLK
                     WIEAGETLGYSCTFKATRPTLVATLPVGYHDGYVRALSNQGRVIVRGQFAPVVGRISM
                     DLTLLDVTDVPGIRLHDQVTLIGAEGKLSLTAEEIAKTADTISYEITCGISARVPRKY
                     RGESGRGKG"
     gene            complement(12716..14080)
                     /gene="dnaB"
                     /locus_tag="VGB76_10565"
     CDS             complement(12716..14080)
                     /gene="dnaB"
                     /locus_tag="VGB76_10565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY0099380.1"
                     /translation="MVKNFASDSMRDQMLERPLPHSAEAERAILGAIILDNGLVNQAI
                     ELLKPDDFYVRAHYHLFRAMISMSERGAEINPILIGEELRRDGSLEQVGGIAFISELT
                     YGLPHFSNIVHYAKLVHDASMLRQLVKVANKITSEALEAEDDASIILDHAEQMIFALA
                     DERTRQGFSPVKPIADAILEKVQELGGRGAVLTGLTTGFNELDTMTSGLQPSDLIIVA
                     ARPSMGKTSLCLTLAQNAAIQANAVVGVFSLEMSKESLVMRMLCSEGHVDAHRFRAGF
                     LSRDEWARLAGALGTLSETKIFIDDTPGISVLEMRAKARRLAAEQKRLDLIIVDYLQL
                     MSGSSRRSESRQQEVSQISRELKGLAKELNVPLVALSQLSRAPEARTDHRPQLADLRE
                     SGAIEQDADVVAFIYREEQYNRTEENAGLAEVIIAKQRNGPTGTVKLAFLKEFTRFEN
                     MWRE"
     gene            complement(14255..14827)
                     /gene="rplI"
                     /locus_tag="VGB76_10570"
     CDS             complement(14255..14827)
                     /gene="rplI"
                     /locus_tag="VGB76_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY0099381.1"
                     /translation="MANTQVLLREDIDNLGARGEIVKVRAGYARNYLLPRKLAVQATA
                     SNVKQIEQERGALLRKEATERAGAEAQAGQMNALRLTFERKVGEHGMLYGSVTSMDIQ
                     EALRAQGYEFDRRRIHLAEAIKETGEFSVRVRLHREVNVEIPVIVTPEGGAVAAGEAT
                     AAAPEAATPEADATAGASTEATGAAGEGGE"
     gene            complement(14849..15103)
                     /gene="rpsR"
                     /locus_tag="VGB76_10575"
     CDS             complement(14849..15103)
                     /gene="rpsR"
                     /locus_tag="VGB76_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY0099382.1"
                     /translation="MGGGRGGGGGARGRFRRRKVCRFCLDKVDIIDFKDVKLLTNYIP
                     ERSKVLPRRISGSCAPHQRMLAEAIKRARNIALLPYAADS"
     gene            complement(15351..15794)
                     /gene="rpsF"
                     /locus_tag="VGB76_10580"
     CDS             complement(15351..15794)
                     /gene="rpsF"
                     /locus_tag="VGB76_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY0099383.1"
                     /translation="MAEKRIYEVVFIIDPATGEEDSTRLVENLQKIVTDQGGAITKSE
                     SMGRRHLAYKIGRNTEGQFMLFEIEGSGGEIAELERRMRVSDQVMRYLTVRVDEDRRR
                     AEKFKERRARKASRRPEKGSARGSGSGAATAVAAAPVAVADEDDE"
     gene            complement(15937..16503)
                     /gene="pth"
                     /locus_tag="VGB76_10585"
     CDS             complement(15937..16503)
                     /gene="pth"
                     /locus_tag="VGB76_10585"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY0099384.1"
                     /translation="MLIVGLGNPGAQYEWTRHNLGFMVVDLLAHAGGRDVKRAECRAL
                     VGRAEIENRGVELVKPQTYMNLSGESVQCLLRKRELKAAGSLIVISDDLALPFGTLRL
                     RARGSSGGHNGLKSIIAQTGTDEFIRLRIGIQPEHPLADAKRYVLERFPPSARGELDK
                     VLERSADAIRAVVRDGIDKAMATYNSDK"
     gene            complement(16599..17264)
                     /locus_tag="VGB76_10590"
     CDS             complement(16599..17264)
                     /locus_tag="VGB76_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEY0099385.1"
                     /translation="MAEKKEFIINAARREGRGKNDARRLRASGRVPLTIYGGEGDSVS
                     ASASLAELAAILRTESGHNTIFTLDVDGEGGHDVMFLDRQIDPVRGRLVHADLRRLVR
                     GQKIEVAVPLHLTGEPIGVTEGGGVLEQMVREINIRVRPRDIPDSIDLDVSNLGVHDV
                     LHVSDIPADEKIEILNEPEMVLATVGIVQDEPVETPATTDEEGAEPEVIGKGKKDDEG
                     DEA"
     gene            complement(17312..18259)
                     /locus_tag="VGB76_10595"
     CDS             complement(17312..18259)
                     /locus_tag="VGB76_10595"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY0099386.1"
                     /translation="MSTTGGIKVFSGNANRELTREICSELGCDPGSATTERFSDGEFN
                     FQITENVRGGDVFIVQPTCPPTDANLMELLVMLDAFRRSSAERVTAVIPYYGYARSDK
                     KDRPRVSIAAKLVANLIATAGADRILTMDLHAAQIQGFFDIPVDHLYAAPVFIGHYQA
                     NPMARLTVVAPDTGGAERARAYAKRLNAELALCDKRREKANVAEVMNVVGDVRGRNCL
                     IVDDMCDTGGSLTKVAKALKDAGADRIHACFTHAVLSGRACEQLTKSDIEQVVTTNTI
                     PFDGKENELDNIEVLSIAPLLAKAIKSIHEETSVSSLFV"
     gene            complement(18362..18433)
                     /locus_tag="VGB76_10600"
     tRNA            complement(18362..18433)
                     /locus_tag="VGB76_10600"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18399..18401),aa:Gln,seq:ttg)
     gene            complement(18478..19386)
                     /gene="ispE"
                     /locus_tag="VGB76_10605"
     CDS             complement(18478..19386)
                     /gene="ispE"
                     /locus_tag="VGB76_10605"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY0099387.1"
                     /translation="MPETRFTLPSFAKINLTLRVLGRRADGYHELHTVFQTISLRDEL
                     TFRSRDDEQLELTCDAPDIPCDETNLVHRAAVALRERYGVSRGASVELRKKIPAGGGL
                     GGGSSNAAVTLVGLARLWEIETERQTLAEIGASLGADVPFFLTGGTALGTGRGTDIHT
                     LADLPPRHLLLITPDVNVSTAEAYKSLNAPALTKPLSPANLTVSRVQAEISGSLHAML
                     ENDFERVVYRLYPQIERARSSLVAAGAGKALLSGSGASVFALFDSPEERERVSGRLQV
                     ETNWQVFACTTVARATYVEAFGADRF"
     gene            complement(19518..20189)
                     /locus_tag="VGB76_10610"
     CDS             complement(19518..20189)
                     /locus_tag="VGB76_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0099388.1"
                     /translation="MTRYNLFPCPGLRVRRHARFALPVFACLFAAGMFSAVEAQTKAV
                     RPVARLIGVTNGGVSNASRSEACETNARLMNASMTSAASRPRTARVNASSDEQRAFDL
                     INRERRARGESALVWDAELTRMARLHSENMARQNFFDHTNPQGENMTMRARACGVCGW
                     SAIAENIAYNSGYDDPAAFAVQRWLASPKHRANMLRDGFTHSGVGVARAADGRVFFTQ
                     VFVTR"
     gene            complement(20380..>20857)
                     /locus_tag="VGB76_10615"
     CDS             complement(20380..>20857)
                     /locus_tag="VGB76_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099389.1"
                     /translation="QPQLVTQALVMLSSAQERAGDTKGAEESLRRILSKSPNDATALN
                     NLGYFLTERGERLPEALDMIKRAVKSEPTNPNYLDSLGWVYFKLGQLDEAERYLNDAA
                     RRNTRSSTVQEHLGDLHHKRGQTEQARAFWRKAIGLTTDASDITRLKAKIGSEAAK"
BASE COUNT         3783 a         6573 c         6299 g         4202 t
ORIGIN      
        1 aaagcatttc actctgtgcg cccctctctc tgcatcccgg cgttgagttt tgacattatt
       61 acgcttccag ttgcgcgtcc agagtgatct tcgcgttgag cacacgcgag acgggacaac
      121 cggccttcgc gttgttggcc gccgtgtcga actgttcctg actcgcgccg ggcacgcgcg
      181 ccgcgacctg caagtgaact tccgtgatcg tgaagttgcc ttccaccttg tcgagcgtga
      241 cggtcgcggt cgtcctgatg ctctcggcgg tgaggtctgc tttgccgagt tggccggaga
      301 gtgccatcga aaaacacccg gcgtgcgccg ccgcgatcag ttcttcaggg ttcgtgccga
      361 tgccgtcttc gaagcgcgtg gagaatgaat attgcgtctc gctgagcacg ccactgttgg
      421 ttgagatcgt gcccttgccg tctttgagtc cgccgcgcca ttcggcggat gcgttacgtt
      481 tcatcgtttg ctccagttgg ttgagttgat aaatacgact gcgagattat ttcgccatcg
      541 cgtcggtcac ttgaatctcg cctttgataa tcttctgccg cgcggtttcg acttcctgca
      601 agactgccgg atcgatgagc ggtcggttga actcgtcgag cgaaaaggcg acgccgtctt
      661 tgtcgagtcc gaagaccttg ataccgcccg tgaaccgttt gttcttcacg tcgttgacga
      721 tgtcgtagac ggcgttgtcc acgcgcttga ccatgctcgt caggacgtag ccgggcttga
      781 ccatgttctg gttcgagtcc acgccgatga cgaaatgcgt ggcgcgcccg tccgcacctt
      841 tgcccgcctg ctccaccgcg tcgaacgcgc cgaggccgga gttgcccgcc gccgtgaaga
      901 tcacgtccgc gcctttgccg atctgcgaga gcgcgagttc cttgcccttg ccggggttgt
      961 tccacgcgct gtccgtgacg ccgacgaagt ttctcacgac gcgcacgctc gggttcacgg
     1021 cctgcgcgcc ctcttcgtag cccttcgcga aacggtgaat cagagggatg tccatgccgc
     1081 cgacaaaacc gagcacgccc gtcttcgtcg tgcgcgccgc gatcatgccg acgaggtagg
     1141 aaccctgatg ctctttgaag acgagcgagt cgacgttcgg cagttggctc acgccgtcaa
     1201 cgatggcgaa gttgatgtgc gggtagtcgc gcgcgacgat ctccatgatc ggggcttgcg
     1261 cgaagccgac gccgatgacg agatcgtagc cgcgctcggc aaaggcgcgc atcgcgggtt
     1321 cgatggaggt gggattgccc ggctccacgt cgcgcaggac gatgccgagt tctcgctcgg
     1381 cgcgctgcac gccctcgaag gccgccgcgt tgaacgagcg atcatctttg ccgccgatgt
     1441 cgaagacgat cccgacgcgc acgcgcgagc ggtcgtccgc gtcgttggct ttcgtcgcgc
     1501 acgacgaaca gacgagcgcg acgagacaca gcagtacgag aaatttctgt agcttaaact
     1561 tcatgcggtg attacttcct tgacgagcat tagcggcgca tccggcggct cgacgccgat
     1621 ggcgtaggcc gcttgaatcc ttgcgcccgc gcctgcggcc tgcgcatcgt cgcgacaata
     1681 gagcgtgccg agcgcgtcgc ccgccctgag ttcgtcgccg tgcacggcct ccgcgatgaa
     1741 accgacctga aaatctatct cgtcttcgac gcgcgtgcga ccgccgccga tggccgagac
     1801 ggcgaaaccg atctcggcag tctcaacttg ggtgacgaat ccggcgcggg gggattctac
     1861 cttaaccgcg cgcagggtcg aagtctgaag caggagcgac gggtcatcgc acacgcgcgc
     1921 atcgccgccc tgcaactcca cgttacgccg gaaacattcc aacgccgcgc ccgattccaa
     1981 cgcgcgaccg acgcgcacac gcgccgcctc aatcgtcggc tcgacattag cgagcgcgca
     2041 catccgcgcc gtcaattcaa gggagaggtc gaggacggga cgcgcgcccg caggcgtttg
     2101 cccgcgtaaa atctcgatgc actcgtgtac ttcgagcgcg tggccgacgg cgcgaccgag
     2161 cggttgattc atgtcggtga gcagggcttc ggtgcgcacg ccgcaagcgt tgccggttga
     2221 gacgagcgcg tgtgcgagtt cgcgcgcacg cgcttcttcg cgcatgaacg cgcccgtgcc
     2281 cgtcttcacg tcgagcgcga gcgcgtcgag tccggcagcc agtttcttcg acatgatcga
     2341 ggcgacgatg agcgggatcg tctcgacggt cgccgtcgca tcgcgcagcg cgtagagttt
     2401 tctatcggcg ggcgcgatct ccgccgtctg ccccatcatg gcgaagccgc agagtttgag
     2461 cacgcggcga aactccgcga gcgagagttt cacctgatag ccggggatgg attcgagttt
     2521 gtcgagcgtg ccgccggtgt ggccgaggcc gcgccctgag atcatcggga cggcgaggcc
     2581 gcaggcggcg gcgagcgggg cgatgacgag cgaggttttg tcgccaacgc cgcccgtcga
     2641 atgtttgtcg gccttgggca gagggatgtc tgagaagtcc agcacttcgc ccgaatggag
     2701 catcgcctcg gtgatcgtgc gctgctccgt ctcgtccatg ccgcgacaga agatcgccat
     2761 cagcaacgcc gccgcctggt agtccgcgaa cgcgccgctc gtcacgccct cgacgaaaaa
     2821 ctcgatctct tcgcgggaga gcgcgccgcc ctcgcgcttt cgccgtatca cgtcctgcgg
     2881 tcgcataaga atagttgttg gatgccggat gctgattttt agattgaaag tctatgcagt
     2941 tttaaggggt cgagatgttt taggcaagcc gtagatcggc atttcgcgca cggcagcggc
     3001 ggctcgctcg tgtcaacccg gcctccgacc ttcgcacggc aggcggcgca gctttcgcgt
     3061 acggcgcaaa gtgcgtccac tctatttcgc tcgccttcat cttaagggcg cgcgtatcaa
     3121 acggcatgag gcttcagatg cgtccgagca ggacgacggc ttgcgcgacg atggcgcgcc
     3181 cttcgcccac ggcgtccaga ccctcgcccg tcttcgcttt cacgctcacg caattcgctt
     3241 cgatggagag cacctgcgcc agacgttcac gcatctccgg tatgtgggga cgcaggcgcg
     3301 ggcgttcgag cgacacgttc gcatccacgt tgaccacttt gaagccgctc tggctcgcca
     3361 gccagacgac gcgcgccagg agttgcagac tgtcggcgtc tttccagcgc ggatcgttgt
     3421 cggggaaatg cacgccgatg tcgccttcgc agagcgcgcc gaggatggca tccgtgatcg
     3481 catgcgccag cgcgtccgcg tccgaatgtc cctccgcgcc tcgctccgac tcgacgagca
     3541 cgccgccgag aatgagcggt cgcccgtcgg ccagacgatg cgtgtcgtgt ccgatgccta
     3601 tgcgaaacat aaaaacagcg ttcagagatt agaggtcgga ggttggcagg aagcagtttt
     3661 tgctttgact gatcttgcct ggccttcgac ctctagcctc cgctttcttt cagtagaatt
     3721 tccgcaagcg cgaaatcttc ggggcgcgtg atcttgatgt tgcgcgcgtc gccttcgatg
     3781 aaggcgacct gtgcgccgca gagttcgacg agcgcgctgt cgtccgtcgc ttcgatctcc
     3841 ccggcgcggg cgcgttcgtg ggcgcggctg agcagttcgt aacgaaagca ctgcggcgtc
     3901 agcgcgcggc gcaaagtctc gcgcgggagc gtgtgcgcga tgcgctcgcc ctctacgact
     3961 ttgatcgtgt cggtgacggg cgcgcagagg acggctgcgc ccatctctcc ggcggcgcga
     4021 attgtgcgtt cgatctctgc gggtgtaacg aaagggcgca cgccgtcgtg gacggcgacg
     4081 atgcgtgtgc gcgtctcgtt caacgtctgc aagccgcgcc cgaccgatgc ggtgcgcgtc
     4141 gtgccgcctt cgacaactcg cgccagcttc gtcaatttga aggggctggc cgagagatga
     4201 aagttcgcct ccgcaaaggg cgcgaccaac acgatctcgt caacggcgtc gcactcttca
     4261 aatcgcctga gagtatgaag cagaagcggg acgcccgcca gcgcacgaaa ctgcttcaac
     4321 ccttcccccc cggcgcgcgt gccccgcccg gcggcgacta tgatggcaac attcataaaa
     4381 cagtgacaag tgacgagtga caggtgacaa gttagaacag taaatcgtgc atcgcgaatc
     4441 gcaggaaaag ccatgaatcg tgaatcgtga atagaagaaa gcagtttttc tctattcacg
     4501 atttacgatt cacggcttta actcgtcact tgtcactgtc tttcagtttt cttccggctc
     4561 gatgctgcgt gcggcgagcg tcgggtgggg gcgcgggtcg cggtagggac ggcgcggggt
     4621 gttgttgccg ttgatgccac gcgtatccgt gttgtggatg ctggctccgt tctcgtgcga
     4681 ctcttcggag agccgcccga agatcatctt cccggcggtt gtctgcaaga cgctcgtgac
     4741 ggagatgtcg gcgttcttgc cgatcatctt gcgcgcgtta tccacgacga ccatcgtgcc
     4801 gtcgtccaga taagcgacgc cctgattgta ttctttgccc tctttgagaa tgaagacgcg
     4861 catcagttcg ccgggcagga cgacgggctt caaggcgttg gcaagttcgt tgatgttcag
     4921 gatcgagacg ccgtgcagtt gcgccacctt gttcaagttg aaatcgttgg tgaccaccac
     4981 ggcatcgagt tgcttgctca gttccagcag cttgagatcg acttcgcgca ccgccgggaa
     5041 gtccgtatcc acgacctgta tgtcgagcgt gttgttggtc tggagtcggt tgagcatgtc
     5101 gaggccgcgc cgcccgcgct gccgcttgtt ggaatcgggc gagtcggcca cctgctgcaa
     5161 ctcgcgcagg atgaactgcg ggatgacgat tgtgccggag agaaatccgg cctcggcgat
     5221 gtctgcgatg cgcccgtcga tgatgacgga agtgtcgagg attttgaaat cgcgccgcac
     5281 cgtcttgtcg ctgaagatgc cgccgagcgc ggagaggtct agatagtcgc ctttggctgc
     5341 gcctacgacg aggccgatgt aggccatcag gaaggtgagc gcgagcgtca ggaaagtctt
     5401 cgtccccggc gcgacggcgt ccgaatgttg cgtgctgatg agcgagccga tgaggtacgc
     5461 gccgacgatt ccgagaatcg aaccgaccgc cgcgccgatt aaagtcttta gagaggcgcg
     5521 ctgaatccgc cgctcgaaca tgatgatggc aaacgcgatg acggcggcga cgcccgccga
     5581 cagcagcggc tggttccgga tcggatgcag cacatatcca gccgccacca gcagcgccgt
     5641 gaagaccaga cgaatgagga tcaaatccgt tttcataacg gcgagaatat taacacgcac
     5701 ttaacgggcg cgtcacgaaa aggtttctca attgcgggtc gcgggttgcg gattggaaag
     5761 tagaaatcag aaggcagaag acggaacaca gaaggtagaa ggcggaacgg tcaaccgttt
     5821 tgcttccgac tcctgtgtcc tgacttctgt attctgttct ctcattcctg aatcagaaga
     5881 gttcgtccaa cgcctcttcc accgagcgga cgccgacgac acgaagaccg aggaggcgtt
     5941 gcaagccttc cgcgttcgag gcgggcacga tcagtttttt aaaacccaac gtctgcgcct
     6001 cgcgtgcgcg cccctccgct tgcatcgccc cgcgcacttc gcccgtcagg ccgacttcgc
     6061 cgaacacggc ggtctgcgcc tcgatgggct tactcttgaa acccgaagcg atggccgcaa
     6121 tcacgccgag gtctgccgca ggctcgtcga tgtcgagtcc gcccgcgacg ttgatgaaaa
     6181 cgtcttcctc cctcagcctg taaccgctga cacgcttttc cagcaccgcg atcagcagcg
     6241 cgatccgccc ctccgcgacg ccctgcgctt tgcgcagcgg gtaattgtac tgcgagccgg
     6301 agacgagcgc ctgcacttcg acgagcatcg ggcgcgtccc ttccatgcac gccgtaacga
     6361 ccgagcctgc ggcgtgcagc ggtcgctcct gcaaaaacat ctccgacgga ttagcgacgg
     6421 gcttgagtcc cgtgcccgtc atctcaaaca cgccgagttc gttggccgcg ccgaagcggt
     6481 tcttcgtcgc gcgcacgatg cgatgattgt gatggcgttc gccttcgaag tagagcaccg
     6541 tgtccacgat gtgctccaga gccttcggac ccgcgattga tccttccttc gtgacatggc
     6601 cgatgaggaa gaccggcacg ccgtgatctt tggcgaagcg caaaaactgc ccggcgactt
     6661 cgcgcacctg cgagactgat cccggcgcgc tctcgatccc cgaagagaag accgtctgga
     6721 tcgaatccac gatcaccgcg cccggctcgt cgcgtttgat ctcgtcgaag atggcttcga
     6781 gattcgtctc cggcagcagg taaaggttct ccgccttgac gcccagacgc tcgccgcgca
     6841 tcttgatctg tcgctccgac tcttcgcccg acacgtagag cacgcgcgcc ccgcccgcgc
     6901 tcaatttgtc agcgacttcg agcagcagcg ttgacttgcc gatgcccgga tcgcccccga
     6961 tcaggacgag acttcccggc acgatgccgc cgccgagcac gcggtcaaac tccgtgatgc
     7021 ccgaagagac gcgcacgtcg tcctgcgatt cgatctctgc ataagcgacg ggcttcactt
     7081 cgcgcgtgcg aaacgcgccc cgccccgccg cgcccttcgg agttgcgcgc acgcgctcct
     7141 cgacaaacga attccactcg ccgcaatccg ggcatttgcc gagcgtcttg gccgattgct
     7201 tcccgcaatt ctggcagacg taaactgtcc ccgccccctt tgccacttct cccgtccttc
     7261 cccgcacgac gcctcgcacg aacgttgcga cgcgtccgcc gtctaaaatt cacttgatag
     7321 ttgttcgcct tcgctactac attgtcgccc tgactcacga tgaaggtcaa acatacttgc
     7381 gccagcgagg tgacgatgac cgaagcacag gcaggcgggc tgggatgtcg catctgcggc
     7441 aacgaagcgg cgaacgctct ccaccacgcg cgtgaaatgt tttacggcac gcgccacccc
     7501 ttcgcctatg tcgagtgcgc cgcgtgcggc acaattcaaa tccaacaggt tccagacctg
     7561 cgcccttact acgcgcaaaa ctattactct ttcgcgcacg cacaagcgga cgcgcagcca
     7621 gccgcgcttg gaataaagag aaatctcgca gcgcgcgtag gagcgtttgt acgccgcacg
     7681 gcgggcgctt attacggcca gcgacgcgca cgcctcggcc aactgcgcca tcccttaggc
     7741 cgctcgctct ccgcgctgct cccgcgcctc gtcgcaggct ttcccgaata tcttttggac
     7801 acgcgcgtta acctgaatct cacgccgcgc tccgccatcc tcgacttcgg ttcgggcgca
     7861 ggctcgacgc ttctcaccct cagcctcttc gggttccgcg acctgacggg cgttgatcct
     7921 ttcgtcgaag ccgacatcga gtatggcgac ggcgtgcgcg tgttgaagac ggaactgtcc
     7981 ggcctctcca gacagtttga tctgattctg gcgaaccatt ccgtcgaaca cgtggcagac
     8041 ccgcgcgcca cgctcgttga gatacgtcgt gtgctcaagc ccggtcgctt cgccgtcata
     8101 cgcatccccg tcgtcgcgca cgcgtgggag cgttacggcg ttaactgggt gcaactcgat
     8161 ccgccgcgcc acctgtttct cttcaacgtc cacactttcg ccggactcgc aacggaagcg
     8221 ggattcgcgt tagaggagct gcgccacgac tccaccgcct ttcagttctg gggcagcgag
     8281 cagtacgcac gcgacattcc tctcacggac gaacgctctt acctcttgaa tcccgccgca
     8341 tccatcttcg aggccgggca gttggccgcc ttcgccgcac aggccgaaga actgaaccgg
     8401 cgcggcgaag gcgatcaggc cgtcttttac ctgcgcaaac cggtgacagg cgactagccg
     8461 acgggcaggg tttcgatgcg ctcaagcgcg cgcttgcacg cctcgcgcat tgcttaagcc
     8521 ttgacgatga agaggtggct gggcgagagg cgcatttcgc gaaacttcga tttgcggggc
     8581 gcgcccgtgc ccatctgcgg gtgtgtgatg tcggccatgc cgagttcgtc cacgagttcc
     8641 ttgacgaagc ccaccatgcc gtagtcgtga ctgtgatatt gcgcgccgag caccgccgcc
     8701 ttcatcttcg acagcaactt gccgacgaga ggcacgcgac cggcgacctg attctgctgc
     8761 aaacgcgaga cggcgcgcgt cgaacgatac aacacggggt tcagactttt gcgcccgcgc
     8821 cggtaagcga cgccgtccag ccagccgtcc cagtagcccg tgccccaatc ttcgatgacg
     8881 tagacgccac cgctcttgag gtggtgctcg aacagatgcc agaagctcgc gcgtgcgagc
     8941 acgccgttgt gcgcgcaatc gtcgatgatg atgtcgaagc ctgcgggggc gttttcccgc
     9001 gcgatgcggt cgaggagttc cgtatcctgt tgcattccct cgtagacgcg tatgcgcccg
     9061 ctcgcgtctt cgagttgcac cgggttaacg tccagcccga cgatccgccc tcgctcgaag
     9121 tagtcgcgcc agagatagag cgagccgcct ttcttgattc cgagttcaaa cagaagcact
     9181 tcccgctcgc gcaactcgcc gaagtagtgt tcgtagttgc gcaggtactc ggcgtgcacg
     9241 gccttgtccg tgtcgtactt cgcggcgatg tgcgcaagat caggcataac gtttacttcg
     9301 cggctcatta acgaccggca gttaaccagc gacaactgac cgttagataa gcggcagttg
     9361 acaaccgacc gtcaatcttc gagcagggtc ttgatcttct ccaccgggcg accgagcacg
     9421 gcgcgttcgc cgcgttcgac gatgggacgt tgcaggagtt cagggtgcgc gacgaggagc
     9481 gcgatgatct ctgcttccgg cagttcgcgc cgctccgggt tcagttcgcg gtagagcgac
     9541 tctttcgtgc gcagcagttc gcgcgcaggc gtctgcatct tggcgatgag ttcgcgcagc
     9601 ttctcttcgc cgagtggcgt ggtgatgtag ttgacgcgct cgaattcgat gccgctctca
     9661 cggagcaggc gatacgcctc gcggcaggtg cttcaagtcg gcttctcgta gatcgtgatg
     9721 ggttcgtcgc tcatttttct ctccaacttt taagggaaga tggcgcgcac gcgcgtcgct
     9781 tcggcgacgc gcccggcggc gagaatgtac gcgccccttg cgcatgtcca tgcggtatct
     9841 gtcgccgacg gcgtagacat gctcgaaggc cgtacgcatg gtctgttcgt gcgcgtcgtt
     9901 cacttgcctg atcttctcga ttcgaatctt agaattactt gggatggtct tcggcgcgct
     9961 cctcgatttc gagaatgtcg ccttcggtga agagatatgt gcgcatgtgc ttatcgaaga
    10021 cggggctgat gcggcgcagg tattcgagcg ccatcatggc gtgctcgatc tcttcgtcgc
    10081 ggttgtgttc gaggatgcgc cgcagttgat cgtccttcgt cacttcggcg cgctggttgt
    10141 accaatccac cgcctccaac tcttcctgca ccgatttgac ggcgcgcgaa aagtttaagg
    10201 tcagttcgct catctgctca taactctcat gccactctgc gctggttgcc acggatgtct
    10261 cctcctgaaa atgcgaagcc taaagatgcg tcgccatcct accttagtct tcaagtcaaa
    10321 gtctaaagct taaaagttgg cgcgcgtggg cgggttcggt ttcgctcaga cgtttcggga
    10381 acaagaagcg aaggggcgga gtctaaattg atcgagtccg ctcgttgcga cccgtttggc
    10441 accggccaca gagaggacta aaagctatgt tcacacactt gatcgaatcc ggctcgcata
    10501 aaaaagagtt cgcgcgcaaa gggcgtttct ttttaggcac gctcggtttc tatggcctgc
    10561 tgctgatggt ggcgggcgtg gcgagcgtct acgcctacaa cgagcaactg ggcaatcagg
    10621 atttggaagt cacactcgtc acgcccgtcc gtgttacgga gatcgagcgc aaccccgacg
    10681 tggtgcgccc cgctcgacca agccacacgt ccggctccgc caatacgggc ggcctgtttc
    10741 gcagggagat tattgccagc acggacaacc ctctgatccc gaagggaatt tccacgcacc
    10801 ccgcgccgcc gccctcgcct cccggcgcga aacagggcac gagagacatc ggcgacggca
    10861 ttgtcatcgg cccccccgac aacggttccg acgacaccgg cacgaccact tcgcgcgtcg
    10921 tcgtgcccgt cgcgaccgat cccgatccgc tgcccccgcc gcgtgcgacg ccgacgcccg
    10981 cgaaacaggt tggacaggtg cggctcccga gcgtcgtgat cagcagcaag atcatcacca
    11041 agcccgcgcc cgtctatccc gccatcgcca gacagacgcg cgtacaggga acggtgacgg
    11101 tcgagatttt gattaacgag caaggccgcg tcatctccgc acaagccacg aacggacacc
    11161 cgctgctgcg cgccgccgca cagtcgtccg cctttcaggc gaagttctcg ccgacatcaa
    11221 tttccggtca gccggtcaaa gtgtccggcg tgatcactta caacttcatc ttgcaataga
    11281 ccgtaaagaa aggagacttc gaagagcaca cagctaaaaa tcaggaaggg acaattcggg
    11341 ggaaagccga agggtcagag gataaaggta agaacaagcg aagctcattg caggcaacgc
    11401 tctgttttta tctttagccc tttccccttc cgctttcccc tctatatttt cgcggcacgc
    11461 gcgcgctgat gccgcaggtg atttcgtagg agatggtgtc tgcggtcttg gcgatctctt
    11521 cggcggtcaa ggagagtttg ccctccgcgc cgatgagcgt cacttgatcg tggagcctga
    11581 tgcccggcac gtccgtcaca tccaggagcg tcaaatccat cgagatacga ccgacgaccg
    11641 gcgcaaactg cccgcgcacg atcacgcgcc cctgattcga caaagcgcgc acgtagccgt
    11701 cgtgataacc gacgggcaaa gtcgccacga gcgtcgggcg cgtcgccttg aacgtgcaac
    11761 tgtagccgag cgtctcgccc gcctcgatcc acttcaacaa aatgatccgc gaacgcatgg
    11821 ccagcaccgg gcgcaactcc ggcggctcgc gctgtgtttg cagcacgtcg cgccagaggc
    11881 cgtagagcac gccgcccggt cgcaccatgt tgccgcgcgc tcgcgggtgc gcgaaagtgg
    11941 ccgcagagtt tgccatgtga aagaaggtcg gcgcgtgccc ttcggcgcga aacgaatcgc
    12001 gcgccgcctc aaagcactcc gactgttccg ccgttaatga atcgcgcgcc gcgtcgtcgg
    12061 cggaggcgaa gtgcgtcatc aggccatcca cgcgcaggtg cccgaaattt ttgagcgcgc
    12121 gtgcaaactc cttcgccgcc ccgccgtagc gcacgccgag ccttccgacg cccgtgtcaa
    12181 tcttcacatg aacatccgcg acgacgcatg cttcccgcgc cgccgattca taagcctccg
    12241 ccatgtcgag cctgtagacg acgggcacga gtttatgttc gagacacgcg cgcgcctgcc
    12301 cctcccagaa gccttcgaga cagaggatcg gctgcacgat gcctgcgcgc cgcaactcga
    12361 tgccctcttc cggcaacgcg accgcaaacc actccgcgcc ctctgccgcc aaccggcgcg
    12421 cgcacctcac cgccccgtgc ccgtacgcgt ctgccttcac gacggccatt actttcactt
    12481 cgccgccgac gcgacggcgc acttcatgaa agttcgcggc gagcgcatcg agatcgatct
    12541 cagcccacgt cgggcgcgcg gcgagagctt cgtcgtcgcc gtcggctccg gtgttttgtg
    12601 tcaggtcgtg ttccgtcatg attaaaaaac ggagtcccgt agtaacgtct gaatcggaca
    12661 ttcaaacgtc gctacgggac cagagacttt cacctgcgcc tgccctgtgg tcggcttact
    12721 cacgccacat gttttcgaag cgcgtgaact ctttgaggaa ggccagtttg accgtgcccg
    12781 tcgggccgtt gcgctgtttg gcgatgatga cttcggccag ccccgcgttc tcttccgtgc
    12841 ggttgtactg ttcctctcga tagatgaagg ccaccacgtc tgcgtcctgc tcgatggcgc
    12901 ccgattcgcg caggtctgcg agttgcgggc gatgatccgt gcgcgcttcc ggcgcacgcg
    12961 agagttgcga aagcgcgacg agcgggacgt tcaactcctt cgccagcccc ttcaactcgc
    13021 gcgagatttg cgacacctcc tgctgccgcg actccgaacg ccgcgacgaa cccgacatca
    13081 actgcaagta atcgacgatg atcagatcga gccgcttctg ttccgccgcg agccgccgcg
    13141 cctttgcgcg catctccagc acggagatgc ccggcgtgtc gtctataaaa atcttcgtct
    13201 ccgaaagcgt gccgagcgcg cccgcgagcc gcgcccattc gtcgcgcgac aggaaaccgg
    13261 cgcggaaacg gtgcgcgtca acgtgccctt ccgaacagag catgcgcatc accagagact
    13321 ctttcgacat ttcgagcgag aacacgccga ccacggcgtt cgcctgaatc gcagcgttct
    13381 gcgcgagcgt caggcagagc gaagtcttgc ccatcgaggg gcgcgcggcg acgatgatca
    13441 agtccgaagg ttgcagcccg gaagtcatcg tgtcgagttc gttgaagccg gtcgtcagcc
    13501 cggtcagcac tgcgccgcgc ccgccgagtt cctgcacctt ttcgagaatc gcatcggcga
    13561 tgggtttgac gggtgagaag ccctggcggg tgcgctcgtc tgcgagcgcg aagatcatct
    13621 gctcggcgtg gtcgagaatg atcgaggcgt cgtcttccgc ttcgagcgct tcgctcgtga
    13681 tcttgttggc gaccttgacg agttgcctga gcatcgacgc atcgtgcacc agtttggcgt
    13741 agtgcacgat gttcgagaaa tggggcaggc cgtaagtgag ttcggagatg aaggcgatgc
    13801 cgccgacctg ttcgagcgaa ccgtcgcgcc ggagttcttc accgatgagg atcgggttga
    13861 tctccgcgcc gcgctcgctc atcgagatca tggcgcggaa gagatgatag tgtgcgcgga
    13921 cgtagaagtc gtcgggcttt aagagttcga tggcctgatt gacgaggccg ttatcgagga
    13981 tgatggctcc gaggatggct cgttcggcct ccgcgctgtg cggaagcggg cgttcgagca
    14041 tttgatcgcg catcgaatcg gaagcgaaat ttttgaccat cgggagactc gggagctagt
    14101 gcaggaagac cgaataaaaa actgctgtta gcagcacgcg gggtcgctct caaacaggaa
    14161 accggaacgg gcgagagcga cgcgctgaca gcatactata ggccaagcgg gcgcgacaac
    14221 gtttagttgc cgcgcccgcc cgtctgtctg agttttactc gccgccttcg cccgccgcgc
    14281 ccgtcgcttc cgtggaagca ccggcagtcg cgtcggcctc tggggtcgcc gcttcgggcg
    14341 cagccgccgt cgcttcgccc gccgccacag cgccgccttc gggcgtgacg atgacgggaa
    14401 tctcgacgtt cacttcgcga tgcaggcgca cgcgcacgga gaactcgccc gtctccttga
    14461 tcgcttccgc gagatggatg cggcgacggt cgaactcgta gccctgtgct ctgagggctt
    14521 cctgaatgtc catcgaagtg accgagccgt agagcatgcc gtgttcgccg accttgcgct
    14581 cgaaggtcag gcgcagagcg ttcatctgtc cggcctgcgc ctcggctccg gcgcgctccg
    14641 tcgcttcctt acgcaagagc gcgccgcgct cctgttcgat ctgcttgacg tttgaagccg
    14701 tcgcctgcac ggcgagtttg cgtggcagca ggtagttgcg cgcgtagccc gcgcggactt
    14761 tgacgatctc gccgcgcgct cccaggttat caatatcttc acgcaagagg acttgtgtgt
    14821 ttgccataat cttttgagtc cctccttttt acgagtctgc ggcgtagggt aagagcgcga
    14881 tgttgcgcgc gcgtttgatg gcctcggcca gcatgcgttg atgcggcgcg cacgatcccg
    14941 aaatgcgacg cggcaaaact ttgctgcgct ccgggatgta gttcgtcaac aacttcacgt
    15001 ccttgaaatc tatgatgtca actttatcca gacagaagcg gcagactttg cggcgacgaa
    15061 acctgccgcg cgcgccgcca ccgccgccgc gacctccgcc catgccgcca ccgccgccga
    15121 agcgaccgcc acggtcgtcg tcatttccga aatcacgttc acgatcacga tcaaaatttc
    15181 tcgacataat tttcctctct caaactctcc ggccaccaac tggccgttca ggcgactcac
    15241 gcagcgcgct ggcgcagaca agttatcaac tcgaaaggcg cgcccgttgt ggccgcgaaa
    15301 agttcacgcc cgctccagcc tgctgcggcg gcgcgacgcg tgtgcgcggt ctactcgtcg
    15361 tcttcgtctg cgacagcgac cggcgcggcg gcgacagccg tagccgcacc gctaccgctt
    15421 ccgcgtgcgc tgcccttctc cggacgcctg ctcgccttgc gtgcgcgacg ctccttgaac
    15481 ttctccgcgc ggcgacggtc ttcgtccacg cgcacggtga gatagcgcat cacttgatcg
    15541 ctgacgcgca tgcggcgttc gagttcggcg atctcgccgc ccgagccttc gatctcgaag
    15601 agcataaact ggccttcggt gttgcgcccg atcttgtagg ccaagtgcct gcgccccatg
    15661 ctctcggatt tggtgatcgc gccgccttga tcggtgacga tcttttgcag gttttcgacg
    15721 aggcgggtcg agtcttcttc gcccgtcgcc gggtctatga tgaagaccac ttcgtaaatt
    15781 ctcttctcag ccaaaattca tcctccttat ggatgtctag cctcggcctt gtggcggaag
    15841 caagaaggaa aagaacagtg acgagtgaca ggtgacgagt gacgggttaa agcaaaactt
    15901 atcttgttcc tgctttaact cttcacttgt cacccgtcac ttgtcactgt tgtaagttgc
    15961 cattgctttg tcaatgccgt ctcggacgac ggcgcggatc gcgtctgcgc tccgttctaa
    16021 aactttgtcc agttcgcccc gtgcgcttgg cgggaagcgt tcgaggacgt atcgcttggc
    16081 gtctgcgagg gggtgttcgg gttgaatccc gatgcgcaga cgtatgaact catcggttcc
    16141 cgtctgcgcg atgatgctct tcaaaccgtt gtggccgccg ctcgaccctc gcgcccgcag
    16201 gcgcagcgtg ccgaaaggca acgcgagatc gtcgctgatg acgatcaaac taccagccgc
    16261 tttcaactca cgcttgcgca gcaggcattg caccgactcg ccgctcagat tcatgtaagt
    16321 ctgcggcttg acgagttcga cgccccggtt ttctatctcg gcgcgtccga cgagcgcgcg
    16381 gcactcggcg cgtttcacgt cgcgcccgcc cgcgtgcgcc agcaaatcca cgaccatgaa
    16441 gccgagattg tggcgcgtcc attcatattg cgcgcccgga ttgcccagcc cgacgataag
    16501 catgagaaaa caggggaagg gatagagggg aaaggaagag aaaggagacg cgggataagc
    16561 acccgccgcc cttcgcctcc cccttttccc ggactccgtt aggcttcgtc gccctcgtcg
    16621 tccttcttgc ccttgccgat aacttccggc tccgcgccct cttcgtcagt cgtcgcagga
    16681 gtttcgaccg gctcgtcctg gacgatgccg acggtggcga gcaccatctc cggctcgttc
    16741 agaatttcga tcttctcatc cgccggaata tccgaaacgt gcagcacatc gtggacgccg
    16801 aggttggaga catcgagatc aatcgagtcg gggatgtcgc gcgggcgcac gcggatgttg
    16861 atttcacgca ccatctgttc gagcacgccg ccgccctcgg tcacgccgat gggttcgccc
    16921 gtcaggtgca gcggcacggc gacttcgatc ttctggccgc gcacgaggcg acgcaaatcc
    16981 gcgtgcacca gacgaccgcg caccggatcg atctgccggt cgaggaacat cacgtcgtgc
    17041 ccgccttcgc cgtccacgtc gagcgtgaag atcgtgttat gcccgctctc cgtgcgcagg
    17101 atcgccgcca gttccgccag cgaagcggaa gccgacacgg aatcgccttc gccgccgtag
    17161 atcgtcaacg gcactctgcc gctcgcgcgc aggcggcgcg catcgttttt gccgcgtcct
    17221 tcgcggcgcg ccgcgttaat aataaattct ttcttctctg ccataatttc aactcgctct
    17281 ttctagcgtg tgcacgctca gtttttgccc ttcacacgaa gagcgaagag acgctcgtct
    17341 cctcatggat cgacttgatc gctttcgcca gcagcggcgc gatactcaga acctctatat
    17401 tatccaactc gttctccttc ccatcgaaag ggatcgtgtt ggtcgtgacg acctgctcga
    17461 tgtccgactt ggtcaactgt tcgcaagccc gccccgacag caccgcgtgc gtgaagcagg
    17521 cgtgaatcct atccgcgccc gcatctttta aagccttcgc caccttcgtc aaagacccgc
    17581 ccgtatcgca catatcgtcc acgatcaggc aattgcgacc gcgcacgtcg ccgacgacgt
    17641 tcatcacttc ggccacgttc gccttctcgc gccgcttgtc gcagagcgcg agttcggcgt
    17701 tcaggcgttt ggcgtaagcg cgtgcgcgct ccgcgccgcc cgtgtcgggt gcaaccacgg
    17761 tcaggcgcgc cataggattc gcctgataat ggccgatgaa gacgggcgcg gcgtacaggt
    17821 ggtcaaccgg aatatcaaag aagccttgaa tctgcgcggc gtgcaggtcc atcgtcagga
    17881 tgcggtcggc tccggcggtg gcaatcagat tcgcgacgag tttggcggca atcgagacgc
    17941 gcgggcgatc ctttttgtcc gaacgcgcat aaccgtaata cggaatcacc gccgtcacgc
    18001 gttcggcaga cgaacgacga aaggcatcga gcatgacgag cagttccatc aagttcgcgt
    18061 cggtcggcgg gcaggtcggc tggacgataa acacgtcgcc gccacgcacg ttctctgtga
    18121 tctgaaaatt gaactcgccg tccgagaaac gctccgtcgt cgcgctgccg gggtcgcagc
    18181 cgagttcgct gcaaatctcc cgcgtcaatt cgcggttcgc gttgccggag aaaactttga
    18241 tgccgcccgt cgtactcata agaagacagt gacgagtgac aagtgacgag tgacaggtta
    18301 gaacaagttt ttggtattca acttgtcact tgccactcgc tacctgtcac tggcttaaag
    18361 gctggggcgg aaggattcga acctacgaat gccggatcca aagaccggtg ccttaccact
    18421 tggcgacgcc ccaatgctgc tctcacgcgc tcgcgcctga cccgcctgcg cgctgaatca
    18481 aaacctgtcc gcaccgaacg cttccacgta tgtcgcacgc gctacggtcg tgcaggcgaa
    18541 aacctgccag ttcgtctcga cctgcaacct gccactcacg cgttcgcgct cttccggact
    18601 atcaaagaga gcgaagacgc tcgctccgct acccgagagc aaagccttgc ctgcgcccgc
    18661 cgcgacgagc gacgagcgcg cccgctcaat ctgaggatag aggcgataga cgacgcgctc
    18721 gaaatcgttt tccagcattg catgcagtga acctgaaatt tcagcctgca cgcgagaaac
    18781 agtgagatta gcgggcgaaa gtggctttgt caaggcgggc gcgttcaacg atttataagc
    18841 ctccgccgtc gaaacattga catccggtgt gatgagcaga aggtgccgtg gcgggaggtc
    18901 tgcgagcgtg tgaatgtccg ttccccgccc tgtgccgaga gccgtgccgc ccgtcaggaa
    18961 gaagggcacg tccgcgccga gcgacgcgcc gatttcggcg agcgtctgcc gctcagtctc
    19021 aatttcccac aggcgcgcga ggccgacgag cgtgacggct gcgtttgaag aaccgccgcc
    19081 cagaccgccg cccgccggaa tcttcttgcg tagttcgacg ctcgcgccgc gactgacgcc
    19141 gtagcgttcg cgcagggcga ccgccgcacg gtgcacgaga ttcgtctcgt cgcagggaat
    19201 gtccggcgcg tcgcaggtga gttcgagttg ttcatcgtct cgactgcgga acgtgagttc
    19261 gtcgcgcagc gagatggtct ggaagactgt gtggagttcg tggtagccgt cggcgcggcg
    19321 tccgagcacg cgcagagtga gattgatctt ggcgaatgat ggaagtgtga atcgtgtttc
    19381 cggcaaaata gagagaaatt tcaacgggca aaaattcagc gcaaaagttt accggcgggc
    19441 aaaagtttag cggctggcct ctcttaaaat tagagcgcca gccgcgtcgt attttagaag
    19501 tcgctcttcc agcggctcta gcgcgtgacg aacacctgcg tgaagaagac gcgcccgtcg
    19561 gcggcgcgtg ccacgccgac gccggaatgc gtgaagccgt cgcgcagcat gttggcgcga
    19621 tgcttgggcg acgccagcca acgctggacg gcgaaggccg ccgggtcgtc gtagccggaa
    19681 ttatacgcga tgttctccgc aatcgcgctc cacccgcaga cgccgcacgc gcgtgcgcgc
    19741 atcgtcatgt tctcgccttg cgggttcgtg tggtcgaaga agttctgccg cgccatgttc
    19801 tcggaatgca ggcgcgccat gcgcgtcagt tccgcgtccc acaccagagc cgactcgccg
    19861 cgtgcgcgcc gttcccggtt gatgagatcg aaggcgcgct gctcgtcgct cgacgcgttg
    19921 acgcgcgcgg tgcgcgggcg cgaggccgcg cttgtcatcg aagcgttcat cagccgcgcg
    19981 ttcgtctcgc acgcttcgga gcgcgaagcg ttgctcacgc cgccgtttgt gactccgatg
    20041 agtcgggcga cagggcgcac ggctttcgtc tgggcttcga cggcggaaaa cattccggcg
    20101 gcgaagaggc aggcaaagac tggaagcgca aaccgggcgt gtcggcgcac acgcaggcca
    20161 ggacagggga acagattgta gcgcgtcatc gggggttaca actctcttta ttcttaaatt
    20221 tagaaccgag aggagggctt tgctaacggc ctgaccgaaa aggggcaatc gatcaaacta
    20281 aagccagtgt atcgcgcgac cccgacaccg tcaatatgtc atttttacac ggtcgagact
    20341 gaccccgaac aagcaatcgc gcgttacgaa tcgcgtcgct tacttggcgg cctcgctgcc
    20401 gattttggcc ttgagacggg tgatgtccga ggcgtcggtg gtgagtccga tggctttgcg
    20461 ccagaaggcg cgtgcctgtt cggtctggcc gcgtttgtgg tggaggtcgc cgagatgctc
    20521 ctgcacggtg gaggaacgcg tgttgcggcg cgcggcgtcg tttaagtagc gttcggcttc
    20581 gtcaagttgg ccgagtttga aatagaccca gccgagactg tcgagatagt tgggattggt
    20641 cggctccgac ttcacggcgc gcttgatcat gtcgagcgct tcgggcagac gctcgcctcg
    20701 ctcggtgaga aagtagccga ggttgttgag cgcggtcgcg tcgttcggcg acttggagag
    20761 gatgcggcgc agagactctt ccgcgccttt cgtgtcgccc gcgcgttcct gtgcggaaga
    20821 gagcatgacg agggcttgcg tgacgagttg cggctga
//
LOCUS       DASWNS010000298         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_400844, whole genome shotgun sequence.
ACCESSION   DASWNS010000298 DASWNS010000000
VERSION     DASWNS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400844"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..398
                     /locus_tag="VGB76_10620"
     CDS             87..398
                     /locus_tag="VGB76_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0099390.1"
                     /translation="MGNPRERPARLAEKLLQIRIALGLSQSQMLKRVGLGTGYRNYIS
                     QFENGEREPTLAILLEYARAAGVYVDDLIDDEFDLPEKIPGVVRYEGIKRKAGARGKK
                     H"
     gene            complement(489..866)
                     /locus_tag="VGB76_10625"
     CDS             complement(489..866)
                     /locus_tag="VGB76_10625"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY0099391.1"
                     /translation="MLRSQAAMQTFNSRYQNSRRPARTGTLVTVGRVEDVPEGRGATV
                     ELKDGTELALYNVEGRFYAVENFCPHRGAPLADGNLCGTTVECDWHGWRFDLQTGQCL
                     GRPAESLEAYEVRIEDGWIKILV"
     gene            1045..1818
                     /locus_tag="VGB76_10630"
     CDS             1045..1818
                     /locus_tag="VGB76_10630"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY0099392.1"
                     /translation="MKRRILLQATLVAMLSLMFVAAGAAQETTGRGQSEGGAVLAAGA
                     TRVVVSGDSLVQARPDTAMISVAVVTQAQTALAAQQENARRSEAMVRALKSAAGAGAE
                     VETSGYSLQPQYTYRENQQPLIRGYEARNTVSVTLGDLTKVGPVIDAATAAGANNIDH
                     LAFTLRRDEPARDEALGKATREALRKAQVLAQALGGRVGRIIEVQEASAGRPVPIYDM
                     RAMRGGIASQAMEAKTPVEIGTLDIRGQVQLVAEIIINK"
     gene            2178..3146
                     /locus_tag="VGB76_10635"
     CDS             2178..3146
                     /locus_tag="VGB76_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0099393.1"
                     /translation="MSRYRDERDDDFYGDRSRARRAGYDEDRGERERGGYGGAREVGG
                     GGRDYDDYGRRRSTSGGYGDREDYRDERERGGYAGGGVGRRPEGRDYDERPRRYEDDD
                     REGRSIFEEGRDFFRGRRSSSSGETRRGATRSHVRCRDIMTRDVTVATRDTTLEEVAR
                     LMKNEDTGVIPVVDRAETPISTRPVSEVAASTTGLDAPVGTSLEARRVNATIHGNGRL
                     VGLITDRDIVVRAIAEGRDPRTTRAEEIMTEEVHTAHPNDRVIEAIRKMGDKQVRRIP
                     IVDQDRTLRGIISMADVALETNDDRELAEALEEISSGSSFWNRIFG"
BASE COUNT          628 a          931 c         1082 g          570 t
ORIGIN      
        1 tctgatagca acaacattct agtcactgtt ctgactagta gcaagcgacg ggcgtggctg
       61 actgccttat cgggagaaaa atcctgatgg gcaatccgcg tgagcgaccc gcccggctcg
      121 ccgaaaagtt gcttcagata cgtatcgcgc tcggcctttc gcagagccag atgctcaagc
      181 gtgtcggact tggaacgggt tatagaaact acatttcaca atttgaaaat ggggagcgtg
      241 agccaacgct tgcgattctt ttagaatacg cacgcgcggc gggcgtctat gttgacgatc
      301 tgattgacga cgagtttgac ttgcctgaaa agattcccgg cgtggtgagg tacgaaggaa
      361 tcaagcgcaa ggccggggcg cgtggcaaga agcattgaac ggcgcgggca atgaattcgg
      421 gcgcgtccgg tcttttatga attggcctcg ccgcttcctt ccgccgcccg caaatcttgc
      481 aacagcactt acactagaat cttgatccag ccgtcttcga tgcggacttc gtaggcttcg
      541 aggctttcgg cggggcggcc tagacactgg ccggtctgta ggtcgaaacg ccagccgtgc
      601 cagtcgcatt cgacggttgt gccgcagagg ttgccgtcgg cgagcggcgc gcccctgtgc
      661 gggcagaagt tctcgacggc gtagaaacgg ccttccacgt tgtagagcgc gagttccgtg
      721 ccgtctttga gttcgacggt tgcgccgcgt ccctccggca cgtcttcgac gcgaccgacg
      781 gtgacgagcg tgccggtgcg cgccgggcgt ctggagtttt gatatctgga attgaaggtt
      841 tgcatcgctg cctgagagcg taacaaaatg gtctgccgtt aggcaaaagc gcgcaaggtt
      901 cgggcgcggc gcgcgttcag gagaggcggg cgcggaagga agagtttcgc gcgtcgagtc
      961 acggacggta ctctctcgcc gccgcgctgg ccgggacgct cgccgcgccg cgaaacgtaa
     1021 agttgggaaa ggaaacggag agacatgaaa agaagaatct tgttgcaggc aacgctggtt
     1081 gccatgttga gtttgatgtt cgtggccgca ggagcggcgc aggagactac ggggcgcggg
     1141 cagagcgaag gcggcgcggt tttggcggcg ggagcgacgc gcgtggtcgt ctccggcgat
     1201 tcgctcgtgc aggcgcgacc ggacacggcg atgatctccg tcgcggtcgt gacgcaggcg
     1261 cagacggcgc tggccgcaca gcaggagaac gcacggcgga gcgaggcgat ggtgcgcgcc
     1321 ttgaagtcgg cggcgggcgc gggcgcggaa gtggagacga gcggctacag tttgcagccg
     1381 caatacactt atcgcgaaaa tcagcagccg ctcatcagag gttatgaagc gcgcaacacc
     1441 gtgagcgtga cgctcggcga tctcacgaag gttggacctg tgattgacgc ggcgacggcg
     1501 gcgggcgcaa acaacataga ccatctcgcc ttcaccctgc gccgcgatga acccgcgcgc
     1561 gacgaggctc tcggcaaggc gacgcgcgag gctctgcgca aggcgcaggt gttggcgcag
     1621 gctttgggcg ggcgcgtcgg gcgcatcatc gaagtgcagg aggcgagcgc ggggcgtccc
     1681 gtccccatct acgacatgcg cgcgatgcgc ggcggcattg cctcacaggc gatggaagcg
     1741 aaaacgcccg tcgagattgg cacgctcgac atacgcggac aggtgcaact cgtcgcggag
     1801 atcatcatca acaagtagaa gcgcgcaagg cgaaagtgtg tgcgaaggcg tgagagcttt
     1861 tccgcccgcg ctttcgcctt tcgcctcccg cccttcacct ttcgccttcc gcctttcgtt
     1921 tctctacttc cgtttttcgc cctccacctt ttgccttttc acttttaact tctataattg
     1981 cgggcgttcc ccttggagtc tgaaaagtgc gcgcaccggg cgcgtgcggc gaacgtcgca
     2041 gcgtgcgccg tcggcatgcg gattgtcgcc gccgtcgctg cggcagagga cgtttgtgct
     2101 tccggtcgca acgccggagc ggtgtcgtcg tcggcgcgcc gcactttcat ctttggagat
     2161 catttcagga ggagatcatg tcgagatacc gtgatgagag ggacgatgat ttttacggcg
     2221 accgctcgcg tgcgcggcgc gccggctacg acgaagaccg tggcgagcgt gagcgcggcg
     2281 gctacggcgg cgcgcgtgaa gtcggtggcg gcggacgcga ttatgacgac tacgggcgca
     2341 gaagaagtac cagcggcggc tacggcgacc gtgaagatta tcgcgacgag cgcgagcgcg
     2401 gcggctacgc aggcggtggc gtcgggcgtc gtcccgaagg gcgtgactac gacgaacgac
     2461 cgcgccgtta tgaagacgac gaccgcgagg ggcgttcgat ctttgaagag gggcgcgatt
     2521 tttttcgcgg acgccgcagc agcagtagcg gcgagacgcg gcgcggcgca actcgctcgc
     2581 acgtccgttg ccgcgacatc atgacgcgcg acgtgacggt tgccacgcgc gacacgacgc
     2641 tggaagaggt cgcgcgcttg atgaagaacg aagacacggg cgtgatcccc gtcgttgatc
     2701 gcgccgagac gccgatcagc acgcgccccg tgagcgaggt ggccgcttcc accaccggac
     2761 tcgatgcgcc cgtcgggacc agcctcgaag cgcgccgcgt gaacgcgacg attcatggca
     2821 acgggcggct cgtcggcttg atcacggaca gagacatcgt ggtgcgcgcc atcgccgaag
     2881 gccgcgaccc gcgcaccaca cgcgccgaag agatcatgac cgaagaggtg cacaccgcgc
     2941 atccgaacga ccgcgtgatc gaagccatac gcaagatggg cgacaaacag gtgcgccgca
     3001 tccccatcgt cgatcaagac cggacgctgc gcggcatcat ctcgatggcc gacgttgcgc
     3061 tcgaaaccaa cgacgaccgc gaactggccg aagccctcga agagatatcg agcggctctt
     3121 cgttctggaa cagaatcttc ggataacaga gtaatgagtg atgagtgatg agtcagaacc
     3181 aaagacgtgt ctttcactca tcactcatca c
//
LOCUS       DASWNS010000299        12551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_271500, whole genome shotgun sequence.
ACCESSION   DASWNS010000299 DASWNS010000000
VERSION     DASWNS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12551
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271500"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..785)
                     /locus_tag="VGB76_10640"
     CDS             complement(225..785)
                     /locus_tag="VGB76_10640"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY0099394.1"
                     /translation="MQLTRTRKIVGGLALAGTMALSGIVGFAQQGTTQGQGGKDHVGR
                     HGGGRGDHKGGRGMGRGFGGGHFAANLNLTDAQKEQMKQIAARYRESAKALRGQRGGG
                     ERRGGSDAFNGGTFNEAEVRAAAQARANARVEMEVQRARMMSEMYAVLTAEQKAQLAA
                     ERQQREQRRQERRTRRNANAAPRSTT"
     gene            complement(990..1748)
                     /locus_tag="VGB76_10645"
     CDS             complement(990..1748)
                     /locus_tag="VGB76_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099395.1"
                     /translation="MSDWQGERTGALREFFGLEASRELVAPEAASFELPTEMAEGHRH
                     FNIEWHLIPSAEAVRFDDAYLARLYPSAPRDLAKTREHGHSYREQLFNGHGRHQGRII
                     GVETTLKPRYLPGNRQFYGTTYGHDPLADPFVAYMGRAGMMNGTRYAHNYLSLREFIR
                     VVNEDWRQRGLLPVNYRVTVCPPAVFNLVGTIFHPEWSATETLELGFYRDAQGNATCF
                     AVGSNSPGDFSYITEIAGEGDWSLAGFRLALVPE"
     gene            2007..3356
                     /locus_tag="VGB76_10650"
     CDS             2007..3356
                     /locus_tag="VGB76_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0099396.1"
                     /translation="MSALFNSPKIRLGALLCLVVLTLAVLGAASCGRPDTKVDVKVEA
                     KEDGKAGAANASDAPPVVVTTAKAEARELPSYIQSTGSLVAEETSDVASQASGQVIAT
                     PVSVGAFVRQGDVIARLNDRDARLRLQQAQAGVQQAIAGVRQAEARLGLRPGGNFDAS
                     AIPEARAASANLEQAQAELRLAEANERRYRALVETGDTAVSTYDQFRTQRDTARARVN
                     AARQQLESALNTARQSNQAIQTAEAAVSSARAQVAIAQKAVADAVIRAPYAGYISNRP
                     VAVGEYVTPSGIIATVLRTNPIKLQLQVQESEVPFITPGMGVSLEVDAHRERKFAGRV
                     TAVNPAIDPVSRAATIEASVENADNALRSGMFATARVVRQGGNRSVFVPRAALVSDQN
                     TQSYRVFVIDKETAKLRVVQIGVEEGGMVQILSGVNADEVVATSNLEQLFEGARVRQ"
     gene            3435..6812
                     /locus_tag="VGB76_10655"
     CDS             3435..6812
                     /locus_tag="VGB76_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0099397.1"
                     /translation="MQKLAEICVRRPVFATVIILLLTVIGGFSFFTLGVDRFPKIDIP
                     TVSVSTSNPGAAPQEIETEITDIIEGVVNTVPGIDEMRSSSAQGRSNVTINFNLEKDP
                     DLATQEVRDKISTVINRLPETADPPVVRKSDPDSQPVLVYAISAPRSSVELTDLVQRQ
                     IQERIESADGVGEVVVFGGRQREIKVYVEPDRLRAYNLSVTEVSNALRQQNLELPGGR
                     LDEGARTVTLRTLSKVTKVEDFNNIVVATRNNYPVKISDIGRVEDSGVDPTSAASLDG
                     VPSVSLGIRKQSGANTIAVIEGVKARMAEIMPLLPQDLKVVVTRDQSEFIETSLHAIE
                     EHLVVGGLLAALIVFLFLWNFRSTVIAALAIPTSIIAAFAVIAALGYSLNQMTMLALT
                     LMVGIVIDDAIVVLENIYRFVEEKGMHPYQAAIEGTREIGLAVMATTLSLLAVFIPVG
                     FMTGIVGRFMSSFGLTSAAAIAVSLIVSFTLTPMLAARWIKPPKESITPEGLPAGAGA
                     ASGGGDSDDHNRHATELDEAAGETETKAHTSSKGGRFYRPIDRSYMWMLRWSMAHRWV
                     VVVLCVAVVASIWPLYKYVGYNFLPDEDESAFQVSLRAPQGTSLAATQSVLDRVARDV
                     REQIPGVKNTLVNAGGFGGGGSPNAGSVQVSLTPVKEREFSQTELIQKTREMIRKRKY
                     PKEYVINASGGSSIGASLGQGRGGSSLGLYISGPEISKLDEYSEKLVERIKKDENFRD
                     ADRSLQLSSPELRIIIDRQRAADLGVRAGDVAQALNTLAAGARVTTFSEGSDQYDVVV
                     RAGEEFRRTRENLSRFTVASSSGGTVGLEKLVRLEEGESPASIDRLNRQRQVTISASL
                     APNGSESDALVAVQRYAQELNMDPAYRTGVTGQSKELQRANQSFMLAFLLSFVFMYLI
                     LAAQFESFIHPVTILLTLPLSIPFALLSTLIAGQRLNIFSALGILLLFGIVKKNAILQ
                     IDHTNELRSHGMERYSAILQANRDRLRPILMTTIALVAGMIPLILGSGAGAATNRSIG
                     VLVVGGQSMCLLLTLLAVPVFYSLFEDAKDSRVWGYVGDRFNNFKGGAGRRLSDAYAG
                     VTGSFARRPKTVTPKVARPLTRQTAKDGYADNVSSGD"
     gene            6793..9126
                     /locus_tag="VGB76_10660"
     CDS             6793..9126
                     /locus_tag="VGB76_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0099398.1"
                     /translation="MFLQVIKSLLRVGGKAHKWGAVFVSALMLCVPVATLAQSQTQTP
                     PSSQPQQTPPAGQRQPPRQPGSPPPATTELPPNSATAPVRPDNEAQQSVQPQTGVEQQ
                     RQQTTPNTQGPRPPSPQTPNASQQTVPGQTQSATPAGTANPQTNTGNAPASGVNTTGG
                     VQQTPGQNVGTSGGIAPADLPTEPPPVAPNFEAASRPLPSAERVGVDIADQLPITLNE
                     AVALALQNNNDIDGSRINVEIAEFNLNAARGVYDPIFSSESYYETRTTPTSSTISGGA
                     NGSVTQKDLTGAMRLGGFSPFAGGNYQLDFSSTRLNTTNQNATLNPQFPTAFTFTYAQ
                     PLLRGLRFDNNRRTIEIAKKNLSLTDAQFRQRAIEVISQVEQSYWDLVFALRNLQVQI
                     DAVKQARVQVESNQRLVAQGVLAPIDIVAANTQVTTFEQNVYTAQESVTRAENTLKTL
                     ILADRTAALWSRPLTPITPVSLEPPRVPLEQAVKAAIDNRPELAQLQTSAEINQIDTR
                     FFRDQSKPQIDLVGTYTSVGLAGSTVSPTINPNTSANAQLRTRVNELSTLAGLTPLET
                     TTGTTGSTVNPNLVGGYPQSLSNLLGNDYPTYRVGVRVELPFRNRTAQANLGRSLAEG
                     RRIVNQRAQTEQLIEADVRNATQTLRSAEARLASAAATRSSAEQQYESEQRQFRAGTT
                     TVFLVLQRQNELLAARGRELQAQTDLNKAIAEFQRATGNTLGANNVSVRASRQLHLSP
                     EATADLDTSLVIPAASPSKPNAGDAVTTTAGTRANEK"
     gene            9116..9808
                     /locus_tag="VGB76_10665"
     CDS             9116..9808
                     /locus_tag="VGB76_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099399.1"
                     /translation="MKNSLAQKSRNRRKLKAAIYYGLALAMFVVLATCAGCQSSRSTN
                     EAARGIVIVNAPAAGEVRRVLVREGMVVGAGTPVVEITVRTEAQNNAPQQQAEDPVAR
                     AGRNIGVAQANIETARAEAVRAEVEVQRLTPLVASGDAPQAQLDGARADYERAQRRLQ
                     EAQNAAQDAQSGLVAARQPPRNPATANPPAPTEKIIVAEATSAGTVSVVNARVGDRVT
                     AGQPLATLRAAQ"
     gene            9896..10261
                     /locus_tag="VGB76_10670"
     CDS             9896..10261
                     /locus_tag="VGB76_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099400.1"
                     /translation="MVSEVSASNIGPRERRKRRLLGIVALTVGVATAFVLVIFEAPRL
                     SRAVIFFPVWLAGLGLLQARERTCIALAARGTCNLDAGEEAIEDEGLIEELRGKSRLI
                     HRRALVTAIAITLLALVFP"
     gene            10567..>12551
                     /gene="topA"
                     /locus_tag="VGB76_10675"
     CDS             10567..>12551
                     /gene="topA"
                     /locus_tag="VGB76_10675"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY0099401.1"
                     /translation="MTKNLVIVESPAKAKTIGKYLGADYRVMASLGHIKDLPSKGLGV
                     DVEHDFATSYEVIPDTKKRNNKKTVTELKKAAREADAIYLAADPDREGEAICQHLAEE
                     IVPKRPKKPSYRVMFNEITKRAVQEAFKEPKQIDENLVDAQQARRVLDRLVGYKVSPL
                     LCRTVGGRLSAGRVQSVALRMVVEREREIEKFLKTEYWTVTANLAANLPPAFDARLLR
                     IGEQTVKTGSFDQEVKKNEVLISNEAQATEIVAEAGAQQFRVAEVTTKERKRNPVPPF
                     ITSKLQQESARKLGFAVKKTMMVAQKLYEGVEIGAEGAVGLITYMRTDSTRVSETALQ
                     EVRDFVGGQYGENYLPEKAIHYRSKKDAQDAHEAIRPSDVRRTPDSLARYLSSEELKL
                     YRLIWQRFVASQMMPAVFDQTTIDIEAGRFLFRASGSVLKFDGFLRVYEEGRDEKSEE
                     DEEAARKLPLVQQGDELKLNSVLPEQHFTEPPPRYSEATLVKALEEKGIGRPSTYAAI
                     MTTILDREYVEKLEGRFHPTALGTTVNDLLVDSFGDLFNESYTARMEQELDEIGEGKL
                     KWTTALHEFYGKFAKDLKIAEVEMKKKKVEAIPTDEICENCGAGMVIKFGRFGQFLAC
                     SNYPECRTTREIAKPQAAAADGNGGSGAAAKAGAGEAG"
BASE COUNT         2457 a         3949 c         3897 g         2248 t
ORIGIN      
        1 tcggtgtccg cacgacgcgg cgattgtttt gatagaccgt taccgtgcgc ccgttgcgcg
       61 tgcggtaagt ggtctgcgcc gccgccgtcg ccgacaggcc gaccacgagc gcgcacatca
      121 ggaatgcgat tctgactctg cttttcattg ctgtcctcca ccaaagattt gtccggggag
      181 agactgtcta tttatttagt tgaaggggag agccgggttc gtgtttacgt ggtgctgcgc
      241 ggggcggcgt tcgcgttgcg gcgcgtgcgt cgctcctggc gtcgctgctc gcgctgctgg
      301 cgttcagccg cgagttgcgc cttctgctcg gcggtcagca cggcgtacat ctccgacatc
      361 atgcgtgcgc gctgaacttc catctcgacg cgtgcgttgg cgcgcgcctg cgctgcggcg
      421 cgaacttcgg cctcgttgaa cgtgccgccg ttgaaggcgt cggagccgcc tctgcgctca
      481 ccgccgccac gctgcccacg cagagccttc gcgctctcgc ggtaacgcgc ggcgatctgt
      541 ttcatctgtt ccttctgcgc gtccgtcagg ttgaggttgg ccgcaaaatg gccgccgccg
      601 aagccgcgac ccatgccgcg cccgccctta tggtcgccgc gcccgccgcc gtgacgcccg
      661 acgtgatctt tgccgccctg tccctgcgtt gtgccctgct gcgcgaatcc gacgatcccc
      721 gacagcgcca tcgtcccggc caacgccagt ccgcccacga tctttctggt acgagttaat
      781 tgcatctgtt cttcatctcc aatgttaagt gaaatatcaa gagccgtatc ggacacagga
      841 gagaatgaag cctcaatccg gcctaccaag atgcaagacg ccgaatttgc gcgggcggtt
      901 acatgttttt agtgatgagc gatgggtgat gagtgatgag ttggaacttt ttgtctttaa
      961 ctcatcactc atcgcccacc actcgtcgct tattcgggga cgagggcgag gcggaagccc
     1021 gccaaagacc agtcgccttc gcctgcgatc tctgtgatgt aggagaagtc gccgggggag
     1081 tttgagccga cggcgaaaca ggtggcgttg ccctgcgcgt cgcggtagaa gccgagttcg
     1141 agcgtctcgg tcgcgctcca ctctggatga aagatggtgc cgacgagatt gaagacggcg
     1201 ggcgggcaga ccgtgacgcg gtagtttacg ggcagcaacc cgcgctgacg ccaatcttcg
     1261 ttcaccacgc ggatgaattc gcgcagggag agatagttgt gcgcgtagcg cgtgccgttc
     1321 atcatcccgg cgcgccccat gtaggcgacg aacggatcgg ccagaggatc atgcccgtag
     1381 gtcgtgccgt aaaactgccg gttgccggga aggtagcgcg gcttgagggt cgtctccacg
     1441 ccgataatgc gcccctgatg gcgtccgtgg ccgttgaaga gttgttcgcg ataactgtgg
     1501 ccgtgctcgc gcgtctttgc gaggtcgcgc ggagcggaag ggtagaggcg cgccagataa
     1561 gcgtcgtcga agcggacggc ctccgccgat ggaatgaggt gccattcaat gttaaagtgt
     1621 ctgtgtccct cggccatttc ggttggtaac tcgaagctcg cggcttcggg cgcgactaat
     1681 tcgcggctcg cttcgagtcc gaaaaattcg cggagcgcgc ccgtccgttc cccctgccaa
     1741 tcagacataa aatgtttacc ccctctgtga catattcgac accctaattc atttaatctc
     1801 aagccgcgcg ggctggcgcg gcaagcgtaa tagcaaattt aatcatctca tctacgacgg
     1861 tagagagaaa tattcataac taaaaatacg aatattcata actgaaaata cggatattca
     1921 ttaagtgaga atgctgtaac cgatctccgg cgcgctgcgt tttcaaacta atgcgggttc
     1981 aaactgaaaa ggtcagaagt ctatttatgt ctgctttatt taactcccct aaaatacgcc
     2041 tcggcgcgtt gctgtgtctc gtcgtgctca cgctcgcggt cttgggcgcg gcttcgtgcg
     2101 gtcgtcccga cacgaaggtt gacgtgaagg ttgaagcgaa ggaggacggc aaggccggtg
     2161 cggcgaacgc ctcggacgcg ccgcccgtcg tcgttaccac ggcgaaggcg gaggcgcgcg
     2221 aactgccctc ctatattcaa tcgacgggca gcctcgtcgc cgaagagact tcggacgtgg
     2281 cctcgcaagc ctccgggcag gtgatcgcca cgccggtcag cgtcggcgca ttcgtccggc
     2341 agggcgacgt gatcgcgcgc ctgaacgaca gggacgcgcg ccttcgtctg caacaggcgc
     2401 aggccggggt gcaacaggcc atcgccgggg tgagacaagc ggaagcgcgg ctcggcctca
     2461 gacccggcgg caacttcgac gcaagcgcca tccccgaagc gcgcgccgcc agtgccaacc
     2521 tcgaacaggc gcaggccgag ttgcgtctgg ccgaagccaa cgagcggcgt tatcgcgcgc
     2581 tggtcgaaac gggcgacacg gcggtcagca cctacgatca atttcgcacg cagcgcgaca
     2641 cggcgcgcgc acgcgtcaac gccgcgcgcc agcaattaga gtccgcgctc aacactgcgc
     2701 gccagagcaa tcaggccatc cagacagcgg aggccgccgt ctcctcggcg cgcgcgcagg
     2761 tcgccatcgc gcagaaggcc gtggcggatg cggtgattcg cgcgccttac gccgggtaca
     2821 tcagcaatcg ccccgtcgcc gtcggcgaat acgtgacgcc ttcgggcatc attgcgaccg
     2881 tcctgcgcac gaacccgatc aagttgcagt tgcaggtgca ggaatcggaa gtgcctttca
     2941 tcacgccggg catgggcgtc tcgctcgaag ttgacgcgca ccgcgaacgg aagtttgccg
     3001 gacgcgtcac ggcggtcaac cccgcgattg atcctgtctc gcgcgcggcg acgattgaag
     3061 cctcggtcga aaacgccgac aacgcgctgc gctccggcat gtttgccacc gcccgcgtcg
     3121 tacggcaggg cgggaacagg tcggtcttcg tgccgcgcgc cgccctcgtc tcggatcaaa
     3181 acacgcagtc ctatcgagtc ttcgtgattg ataaagagac tgcgaaactg cgcgtcgtgc
     3241 agatcggcgt ggaagaaggc ggcatggtgc agatactttc cggcgtcaac gcagacgaag
     3301 tggtagcgac gagcaatctc gaacaacttt ttgaaggcgc acgcgtaaga cagtgaaact
     3361 gttagccgtt agctcttagt tgaaacgaaa agttccggcc cacagctgac agcccgcaaa
     3421 gctcttgagg atttatgcaa aaactagctg aaatttgtgt ccgtcgcccc gttttcgcga
     3481 ccgtcattat tttgctcctg accgtcatcg gcggtttctc tttcttcacg ctcggcgtcg
     3541 accgcttccc gaagattgat attccgaccg tttcggtttc gacctcgaat cccggcgcgg
     3601 ctccgcagga gatcgaaacc gagatcacgg acatcatcga aggcgtcgtc aacaccgtcc
     3661 ccggcattga cgaaatgcgc tccagttccg cgcagggacg ctcgaacgtc acgatcaatt
     3721 tcaatctcga aaaagaccct gacctcgcca cgcaggaagt gcgcgacaag atcagcaccg
     3781 tcatcaaccg tctgccggaa accgccgacc cgcccgtcgt gcgcaagtcc gacccggatt
     3841 cgcagcccgt cctcgtctat gcgatcagcg cgccgcgctc ctcggttgag ttgaccgacc
     3901 ttgtccagag gcagattcag gaacgcatcg aatcggctga cggcgtgggc gaagtggtcg
     3961 tcttcggtgg acgccagcgc gagatcaaag tctacgtcga accggatcgc ctgcgcgcct
     4021 acaacctctc ggtgacggaa gtttccaacg ccctgcgcca gcagaatctc gaactgcccg
     4081 gcgggcggct cgacgagggc gcgcgcacgg tgacgctgcg cacgctgtcg aaggtcacga
     4141 aggtcgaaga cttcaacaac atcgtcgtcg ccacgcgaaa caattacccg gtcaagatca
     4201 gcgacatcgg gcgcgtcgaa gacagcggcg ttgacccgac ttcggcggcc tccctcgacg
     4261 gcgttccgtc cgtctcgctc ggcatccgca aacagtcggg cgcaaacacc atcgccgtca
     4321 tcgaaggcgt caaggcgcgc atggcggaga tcatgccgct gctgccccaa gacctaaagg
     4381 tcgtcgtcac gcgcgaccaa tcggagttca tcgagacgag tctgcacgcc atcgaagagc
     4441 acctcgtcgt cggcggcctg ctcgccgccc tgatcgtctt cctgttcctc tggaacttcc
     4501 gttcgacggt catcgccgcg ctcgccatcc cgacttcgat catcgccgcc ttcgccgtca
     4561 tcgccgcgct cggctactcg ctcaaccaga tgacgatgct tgccctgacc ctgatggtcg
     4621 gcatcgtgat tgacgacgcc atcgtcgttt tggagaacat ctatcgcttc gtcgaagaga
     4681 aggggatgca cccttatcag gcggcgattg aaggcacgcg tgagatcggt ctggccgtca
     4741 tggcgacgac gctcagcctg ctcgccgtgt tcatccccgt cggctttatg accggcatcg
     4801 tcgggcgttt catgtcgtcg ttcggactga cttccgccgc cgccatcgcc gtctctctga
     4861 tcgtttcgtt tacgctgacg ccgatgcttg cggcgcggtg gatcaagccg ccgaaagagt
     4921 ccatcacgcc cgaaggcttg cccgccggag cgggcgcggc gagcggcggc ggagactccg
     4981 acgaccataa ccggcacgcg accgaactcg acgaagctgc gggcgaaact gagacgaagg
     5041 cgcacacctc ttcgaagggc ggacgttttt atcgtccgat tgatcgctct tatatgtgga
     5101 tgcttcgctg gtcgatggcg catcgctggg tggtggtggt gctctgcgtg gcggtcgtcg
     5161 cttcgatctg gccgctctat aaatatgtcg gctacaactt tctgcctgac gaagacgagt
     5221 cggccttcca ggtgagtttg cgcgcgccgc agggcacgag cctcgcggcg acgcaatcgg
     5281 tgctggatcg cgtcgcgcgc gacgtgcgcg agcaaatacc gggcgtcaag aacacgctcg
     5341 tcaacgcggg cggtttcggc ggcggcggtt cgcccaatgc cggttcggtt caggtgagcc
     5401 tcacgccggt taaggagcgc gaattttcgc agaccgagtt gatccagaag acgcgcgaga
     5461 tgattcgcaa gcggaagtat ccgaaggagt atgtgatcaa tgcgtcgggc ggttcgtcca
     5521 tcggcgcgag cctcgggcag gggcgcggcg gttcgtcgct cggcctctac atcagtggtc
     5581 ccgagatcag taagctcgac gagtattcgg aaaaactggt cgaacgcatc aagaaggacg
     5641 agaacttccg cgacgcggat cgttcgctgc aactgagcag cccggagttg cgcatcatca
     5701 tcgaccgcca gcgcgccgca gacctcggcg tgcgcgcggg cgacgtggcg caggctttga
     5761 acacgctcgc cgccggggcg cgcgtcacga ctttcagcga aggctcggat caatacgacg
     5821 tggtggtgcg cgcgggcgag gagtttcgtc gcacgcgcga gaatctgagc cgcttcacgg
     5881 tagcctcttc ttcgggcggc acggtgggct tggagaaact cgtgcgactc gaagaaggcg
     5941 agagtcccgc ttcgattgat cgcctgaacc gccagagaca ggtgacgatc tccgcgagcc
     6001 tcgcgccgaa cggctcggag tcggatgccc tcgtcgccgt acagcgttac gcgcaggaat
     6061 tgaacatgga ccccgcttat cgcacgggcg tcacgggtca gtcgaaggag ttgcagcgcg
     6121 cgaaccagtc gttcatgctg gcgttcctgc tctcgttcgt cttcatgtac ctgattctcg
     6181 cggcgcagtt cgagtcgttc atccacccgg tgacgatcct cttaacgctg ccgctctcga
     6241 tcccgttcgc gctgctgtca accttgatcg cggggcaacg tctgaacatc ttctccgcgc
     6301 tcggcatcct gctcctcttc ggcatcgtga agaagaacgc gattctccag attgaccaca
     6361 cgaacgaact gcgctcgcac ggcatggaac gctacagcgc gatcctgcaa gcgaaccgcg
     6421 accgcctgcg ccccattttg atgacgacca tcgcgctcgt cgcgggcatg atcccgctca
     6481 tcctgggaag cggcgcgggc gcggccacga accgttccat cggcgtgctc gtcgtcggcg
     6541 gtcagagcat gtgtctgctc ttaacgctgc tcgccgtgcc ggtcttctat tcgctgtttg
     6601 aggatgcgaa agattcgcgc gtgtggggct atgtcgggga tcgcttcaac aatttcaagg
     6661 ggggcgcggg gcgtcgtctc tcagacgctt atgcgggcgt gacgggttcg ttcgcgcgca
     6721 ggccgaagac ggtcacgcca aaggtggcga ggccgctcac gcgccagacg gcaaaggatg
     6781 gctatgcaga taatgtttct tcaggtgatt aagagtttgc ttcgcgtcgg cggcaaggcg
     6841 cacaagtggg gagcggtctt cgtctcggca ttaatgttgt gcgtgcctgt ggcaacgctc
     6901 gcgcagagcc agacgcagac gccgccttcg tcgcagccgc agcagacgcc gcccgcgggt
     6961 caacgccagc cgccgcgaca acccggctcg ccgcctccgg cgaccacgga gttgccgccc
     7021 aactcggcca cggctcccgt gcgtcccgac aacgaagcgc agcagagcgt gcagccgcaa
     7081 acgggcgtcg agcaacagcg tcaacagacg acgcccaaca cgcaggggcc gcgcccgcct
     7141 tcgccgcaga cgccgaacgc ttcgcagcag accgtgcccg gacagacgca gagcgcgacg
     7201 cccgccggaa ctgccaaccc gcagaccaac accgggaacg cgcccgccag cggcgtgaat
     7261 acgaccggcg gggtgcagca aacgccgggg cagaacgtcg gcacgtcggg cggcatcgcg
     7321 cccgccgact tgccgaccga gccgccgccc gtcgcgccga acttcgaagc cgcctcgcgc
     7381 cctctgcctt cggcggagcg cgtcggcgtg gacatcgccg atcaattgcc gatcactctg
     7441 aacgaggccg tcgcgctcgc actgcaaaac aacaacgaca ttgacggctc gcgcatcaac
     7501 gtcgagatcg cggagttcaa cctgaacgcg gcgcgcggtg tctatgatcc gatcttttcg
     7561 tccgagagtt attacgagac gcgcacgacg ccgacgagtt cgaccatcag cggcggcgcg
     7621 aacggctccg tcacgcagaa ggatttgacg ggcgcgatgc gcctcggcgg cttctcgcct
     7681 ttcgcgggtg gcaactatca actcgacttc tcttcgacgc gcctgaacac caccaaccag
     7741 aacgcgacgc tcaacccgca gttcccgacg gcctttacgt tcacctacgc gcagccgctc
     7801 ctgcgcggcc tgcgcttcga caacaaccgt cgcaccatcg agatcgccaa gaagaatctc
     7861 agtttgactg acgcgcagtt tcgccagcgc gccatcgaag tgatctcgca ggtcgaacaa
     7921 tcctactggg atttggtctt tgcgctccga aatttgcagg tgcagatcga cgccgtgaag
     7981 caggcgcgcg tgcaggtgga gagcaatcag aggctcgtgg cgcagggcgt gctcgcgccc
     8041 atcgacatcg ttgcggccaa cacgcaggtc accaccttcg agcagaacgt ctataccgcg
     8101 caggagtccg tgacgcgcgc cgagaacact ctgaagacgc tcatcctcgc agaccgcacc
     8161 gccgcgctct ggtcgcgtcc tttgacgccg atcacgcccg tcagtttaga gccgccgcgc
     8221 gtgccgctcg aacaggcggt caaagccgcg attgataatc gtcccgaact cgcgcagcta
     8281 cagacgagcg cggagattaa ccagatcgac acgcgtttct tccgcgacca gtcgaagccg
     8341 caaattgatc tcgtcggcac ttacacctcg gtcggcctcg cgggttcgac cgtctcgccg
     8401 acgatcaatc ccaacacgag cgcgaacgcg cagttgcgca cgcgcgtcaa cgaactctcc
     8461 acgctcgccg gactcacgcc gctcgaaacg accaccggca cgacgggcag caccgtcaac
     8521 ccgaacctcg tcggcggcta cccgcagtcg ctctcgaacc tgctcggcaa cgactacccg
     8581 acttatcgcg tcggcgtgcg cgtcgagttg ccgtttcgca atcgcacggc gcaggccaac
     8641 ctcggtcgct cgctcgcgga aggccgccgc atcgtcaacc agcgcgcgca gacggagcaa
     8701 ctcatcgaag ccgacgtgcg caacgccacg cagacgctcc gctcggcgga agcgcgtctc
     8761 gcctccgccg ccgccacgcg ctcgtccgcc gaacagcaat acgagagcga gcagaggcaa
     8821 ttcagggcgg gcaccacgac cgtctttctc gtcctgcaaa gacagaacga actcctggcc
     8881 gcgcgcggtc gagagctaca ggcgcagact gatctcaaca aggccatcgc ggagtttcaa
     8941 cgcgcgacgg gcaacacgct cggcgcaaac aatgtctcgg tgcgcgcctc tcggcaactc
     9001 cacttgagtc cggaagccac cgccgacctc gacacgtcgc tcgttatccc ggcagcgtcg
     9061 ccgtcgaagc cgaacgcggg cgacgcagtg acgacgacgg cgggaaccag agcgaatgaa
     9121 aaatagtctc gcgcaaaagt ctcggaacag gcgaaagctc aaagccgcga tctactacgg
     9181 cctcgcgctc gctatgtttg tcgtcctcgc cacctgcgcc ggttgccaat cttcacggag
     9241 cacgaacgaa gcggcacgcg ggatcgtgat cgtgaacgcc cctgcggcgg gcgaggtgcg
     9301 gcgcgtgctc gtgcgcgagg gaatggttgt cggcgcaggg acgccggttg tcgaaatcac
     9361 cgtccgcact gaagcgcaga acaacgcgcc tcagcaacag gcggaagacc ccgtggcgcg
     9421 cgcggggaga aacatcgggg tggcgcaggc caacatcgag acggcgagag ccgaagcggt
     9481 gcgcgccgaa gtggaggtgc aacgcttgac gccgctcgtc gcttccggcg atgccccgca
     9541 agcgcaactc gacggcgcgc gtgccgatta tgaacgcgcg cagcggcgtc tacaggaggc
     9601 gcagaacgcg gctcaggacg cgcaatccgg cctcgtcgcc gcgcgccagc cgccgcgcaa
     9661 cccggccacc gccaatcctc ctgcgccgac ggagaagatc atcgttgccg aagccacttc
     9721 cgccgggacg gtgagcgtcg tcaacgcgcg ggtcggcgac cgcgtgaccg cagggcaacc
     9781 gctggcgacg ctgcgcgccg ctcagtagtc aggtcgagca cgagcgagta tggcttccaa
     9841 agtttcagcc tctcataaac gaaggacttc caaagtctct gtctctcgcg agcgaatggt
     9901 ttccgaagtc tcagcttcca acatcggacc ccgcgaacgg cgcaagcggc gactgctcgg
     9961 catcgtggcc ttgaccgtcg gcgtcgcgac ggccttcgtg ctggtcatct tcgaagcgcc
    10021 gcgcctgtcg cgcgccgtca tcttctttcc cgtgtggctg gcggggctgg gcttgttgca
    10081 ggcgcgcgag cgaacctgca tcgcgctcgc cgcgcgcgga acgtgcaacc tcgacgcggg
    10141 cgaagaggcg attgaagacg aaggcttgat cgaagaactg cgcggcaaat cgaggctcat
    10201 acatagacgc gcgctcgtca ccgctatcgc catcacgctc ctcgcgctcg tctttccctg
    10261 agccggagat cggagtgccg cccggctcct ttaatcctct cgcccgcgcc tctgcaactc
    10321 ttccgaaatt aagtctcgcg ccgcttcgcg ctcgccttcg cctccgttgt tcatttgact
    10381 ttagagcctc tatcagtgct attttcatag cccttcaccg cacacacgca agttgaagca
    10441 ggaaaatcag ccatgtgcgc ggccagtaaa aacgcgtttt gcggcatcta aacggtgacg
    10501 gcagagggaa tttaaggact cgtgacggtg tttcgagaga acaccttttg attcgaaggg
    10561 aatggaatga cgaagaattt agtcatagtt gaatcgcctg cgaaggccaa gacgattgga
    10621 aaatatctgg gcgcggacta tcgcgtgatg gcctcgctcg gccacatcaa agacctaccg
    10681 tcgaagggtc tgggcgttga cgtagagcat gatttcgcca cgtcctacga agtgatcccc
    10741 gacacgaaga aacgcaataa caaaaagacc gtcaccgaac tcaagaaggc cgcgcgcgag
    10801 gccgacgcca tctacttggc cgccgaccct gaccgcgaag gcgaagcgat ctgccagcac
    10861 ctcgcggaag agatcgtgcc gaagcgaccg aagaagcctt cctaccgcgt gatgttcaac
    10921 gagatcacga agcgcgcggt gcaggaagcc ttcaaagagc cgaagcagat cgacgaaaat
    10981 ctggttgacg cgcaacaggc cagacgcgtg ctcgaccgtc tcgtcggtta caaagtttcg
    11041 cccttgctgt gtcgcacggt cggcgggcgt ctgagcgcgg ggcgcgtgca gtcggtcgcg
    11101 ttgcgcatgg tggtcgaacg cgaacgcgag atcgaaaagt ttctcaagac ggaatactgg
    11161 acggtgacgg cgaacctcgc ggcgaacctg ccgcccgcct tcgacgcgcg cctgcttcgc
    11221 attggcgagc agaccgtcaa gaccggcagc ttcgatcagg aagttaagaa gaacgaggtt
    11281 ttgatctcga acgaggcgca ggcgacggag atcgtcgcgg aagcgggagc gcagcagttt
    11341 cgcgtcgcgg aggtgacgac gaaggagcgc aagcgcaatc ccgtgccgcc cttcatcact
    11401 tcgaagctgc aacaggaatc ggcgcgcaaa ctcggcttcg cggtgaagaa gacgatgatg
    11461 gtcgcgcaga aactctacga gggcgtggag atcggcgcgg aaggcgcggt cgggttgatc
    11521 acctacatgc gcacggattc gacgcgcgtg tcggagacgg ccttgcagga ggtgcgcgat
    11581 ttcgtcggcg gtcaatacgg cgagaattat ctgccggaga aggcgattca ctaccgctcg
    11641 aagaaggacg cgcaggacgc gcacgaagcc atccgcccgt cggacgtgag gcgcacgccc
    11701 gattctctgg cgcgctatct gagcagtgaa gagttgaaac tttatcgcct gatctggcag
    11761 cggttcgtgg cgtcgcagat gatgcccgcc gtctttgatc aaacgacgat tgatatcgaa
    11821 gcgggacgct tcctgtttcg cgcgagcggg tcggtcttga agttcgacgg cttcctgcgc
    11881 gtctatgaag aagggcgcga tgagaagtcg gaggaggacg aagaggctgc acgcaagttg
    11941 ccgctcgtgc aacagggcga cgagttgaag ttgaactccg tcctgcccga acagcatttc
    12001 accgaaccgc cgccgcgcta ttcggaagcg acgctcgtga aggctctcga agagaaaggc
    12061 atcgggcggc cttccaccta tgccgcgatc atgaccacga ttctcgatcg agagtatgtg
    12121 gaaaagctgg aagggcgttt tcacccgacg gctctgggga cgaccgtaaa cgatctgctc
    12181 gtagatagtt tcggcgacct cttcaacgag tcgtacacgg cgcggatgga acaggaactc
    12241 gacgagatcg gggaagggaa gttgaaatgg acgacggcgc tccatgagtt ctacgggaag
    12301 tttgcgaagg atttgaagat cgccgaagtc gagatgaaga agaagaaggt ggaggccatc
    12361 ccgaccgacg agatatgcga aaactgcggc gcgggaatgg tcatcaagtt cggacgcttc
    12421 ggccagttcc tcgcctgctc gaattatccc gaatgtcgca cgacgcgcga gatcgcgaag
    12481 ccgcaagcgg cggcggctga cggcaacggt ggtagcggcg cggcggcgaa agcgggcgcg
    12541 ggcgaggcgg g
//
LOCUS       DASWNS010000300        16441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_116673, whole genome shotgun sequence.
ACCESSION   DASWNS010000300 DASWNS010000000
VERSION     DASWNS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16441
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116673"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1625)
                     /locus_tag="VGB76_10680"
     CDS             complement(42..1625)
                     /locus_tag="VGB76_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099402.1"
                     /translation="MTIQQIQLTSYDTVVAVTQNSINEMLATFLDGLQKNVALYYTAD
                     AEGNFVPTTPDAAVFTFTGTLGYAVENGAPVNIVQLYNEGQPQTVRYNITFGNAEFKS
                     ISVPPFDVKQSGNEPWIIGFLVNLALKEVSPESLPANVRQAVSRYVGQLSPDMFSIQQ
                     LYLDLNTAAFDTFQNIENMPDLAATLLSGIMRVYLKQQQAQGGTIFGYTLHSTAANQQ
                     PPTFMPTALDFCVTPYTDATGNHSNPGLDTLNYLLMTDHRPLPPNPPSGFGFNWVDDN
                     SVQGTMAVRHDLILNYLIPHFNGILQTISPTVNANAKHDKVTFGQGSGTNSFTMHNPP
                     LSGNVVADYSYKESGKDSHTTGSGVTTIVSSHYSSNCRITFNGDTIHVSGTITCSASE
                     IVSSSETDNETDMPATTYAWYADLRLQMNLADNGSLDIVVVDSDFDSPPTVAGHDLKW
                     WQKVIEGFGGGMTQYVKDFGGLQGDVQTNLDAVLPAIKSVLSTTNDFVFPGANTFAFK
                     NPQFSDDLDLASNITYLST"
     gene            1911..2726
                     /locus_tag="VGB76_10685"
     CDS             1911..2726
                     /locus_tag="VGB76_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0099403.1"
                     /translation="MTRNILVRNNVKVFGKGTQPMLFAHGFGCDQNMWRFVTPAFEEH
                     YRIILFDYVGSGKSDLQAYSPERYATLEGYTGDVLDICAELDLRDVIFVGHSVSTVVG
                     MLASIREPERFARLILVGPSPRYINDPPAYVGGFDRSDIAGLLEVMEKNYIGWANYLA
                     PIVMKNGERPELTRELEESFCSTDPKIARLFAEATFLSDNRDDLPKVPVPSLIMQCSE
                     DSIAPLEVGEYLHRHLPQSTLRLLRATGHCPHMSHPEETVQVIKEYLAARCAA"
     gene            2757..4304
                     /locus_tag="VGB76_10690"
     CDS             2757..4304
                     /locus_tag="VGB76_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099404.1"
                     /translation="MDDLLNTAPCGFLSFNDDGKIMLVNATLLNLLGYEPDELPGRHV
                     ESLLTVASRIFYQTHLFPLLKLHDRVEEVYFSLRAKDGAEIPVLVNAVRRERGGVFAN
                     ECILMHMRQRNRYEDELLKTKRAAEEATRAKDEFLAVVSHELRTPLNAILGWARMLQT
                     GKLDERTGVRALDAIERNAESQNQLIGDLLDFSRIISGKIRLDVGRVDLASVVRASID
                     VVSPAADAKGISLQADLDPEAEAVSGDPERLQQVMWNLLSNAVKFTPQGGRVQVRLTR
                     VDSSVEIAVSDTGEGIDAEFLPYVFERFRQADSATTRRQSGLGLGLAITKHLLELHGG
                     TITAESSGEGQGSTFILRLPTIGVHQPERFSITSGEWQSPASEDHTQPVELARLDGIH
                     VLVVDDEGDARELLTAILTQSGATVTVASNVADALDKLHSLKPDLLLSDIEMANEDGY
                     SLIRKVRVLEEGRGQRIPAIALTAHARSSDRSRAISQGFQLHISKPVEPAELVIAIVN
                     LLDSERS"
     gene            complement(4417..4893)
                     /locus_tag="VGB76_10695"
     CDS             complement(4417..4893)
                     /locus_tag="VGB76_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099405.1"
                     /translation="MRKNISVKFIMAAIALFMVAAVSIPAEAQWGRQARGRGYSKGQV
                     ERIIRRLENQSDRFVRSFDRSLDNSRADGSRREDNLNQRARDLENALDVLRQEFDRTE
                     RYQDTRSQVSSVLNVAEDINRAVRNRRLRGNTERLWAQVRSELNALASVYNLRQLR"
     gene            complement(5142..7784)
                     /locus_tag="VGB76_10700"
     CDS             complement(5142..7784)
                     /locus_tag="VGB76_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0099406.1"
                     /translation="MHIRQLRSWLVRLTAIFGREARERELAEELESHLQMHVEDNIRA
                     GLSPEEARRQALVKLGGVEQTKEEYRRQGGVPLLGDLWQDLRFGARVLLKQPAFTLVA
                     VLTLALGIGANTALFSVINAVLLRPLPFPESDRLVALYETFQPSGQSALSVPNLLDWQ
                     QQNTVFEGIAAYVDGAFNLQSGDSPQRLQGLKVQANYFDVLGIKPRLGRAFLHGEDEA
                     GSERVVVLSDALWRNGFGADPNIVNKTIPLNGQKYTVVGVMPQELSALSSTQLWSPLV
                     FAEGEKTDRRSRSYFTIGRLKQGVTLEQASEQMSLIAARLEQQYPETQAGRGARLRRY
                     EEEIVGDVRQPLFMLMGAVAFVLLIACTNVANLLLARAAGRYREIAVRIALGAGRLRL
                     VRQFLTEGVLLSLAGGAIGVATAWLGLDLLSKLAFSFLPRSSEIKLDLRVLGFTLLVS
                     ILTGVIFGVAPVAQALKTNVQEALKDGGKGSSQGFGGNLMRNTLVVTEIAAAFVLLIG
                     AGLLIKSFAKVQSVDHGLKPEHVLTAKLSLAPERYADGDALRRFHRQVLERVAALPGV
                     ESAGLTTHLPVEQYGTNGYMTVEGKTYAPTQEPLVELRVVSPDYFRAVGVALLRGRMF
                     DARDTQESPLGVVINETMARTIWPGEDPVGKRVSGRPIRPDWVPVIGVVADVKNMGLT
                     QQPAAEFYFNYAQGGEDILRNMTLAVRSRLDPGSLAAAVRREVQTLDPAQPLFDVQTM
                     QAVLDGTVSDRRLNMTLLGVLAALSLVLAVVGIYGVMSYNVTQHTREIGIRMALGAQK
                     TDILKLVLGRGVTLALIGVVSGMLLSLGLTHLMSGLLFGVSATDPATFGVIAVLLFGV
                     ALVACYLPALRAVKVDPLIALRYE"
     gene            complement(7784..8125)
                     /locus_tag="VGB76_10705"
     CDS             complement(7784..8125)
                     /locus_tag="VGB76_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0099407.1"
                     /translation="MGEKTEIWQGTLALMVLKTLEAMGAQHGYGLARRIEQTSGDLLS
                     INYGTLYPALLKLEQEGYVSSEWGVSDNNRKAKYYKLTRAGRRQLAREAREWEQTTAI
                     LARFFAPAKES"
     gene            complement(8232..10478)
                     /locus_tag="VGB76_10710"
     CDS             complement(8232..10478)
                     /locus_tag="VGB76_10710"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY0099408.1"
                     /translation="MLLIRKILTALFGFAALAGHVWIVTATLPNPERLAQSPAHGLRP
                     HAAPRKRALLVGISNYERVEGARSSCPRSDLNWPDLNAATDVEQLELVLRETFGFDEI
                     KVLRTPEETTRRNITGVFRSFLIEQAAPGDIIFFHFSGHGGQVPDDEKHGLNPQVGDE
                     LDGLDETLVPSDYVSPCDPSNDIRDDEFGRLLSELRAKKPASITVTMDACHSGSNTRG
                     ANTVARGRGWHGAAPTVAPGARREESASGMLQSVDARARGLTVLAASRHDQRALETTP
                     GANHPQMGTFTYALIRALRKADAATTYRDLFERITDIIASESPFWSQKPQLEGDTDAL
                     LMSGVSLPPSKQYVEVSVRREQVILAAGYLQGVTAGSQFSIYPAGKDPETSEPLAEAE
                     VVTPQLNTSVLKIVKPAPVESLLEKLRAARAVETRHNYGDMRLKVATLNLDGEAAAVV
                     GRLRELELVNFNLARPDLWDVRICRDKCPDESDSGNAGSAGVKGGFTLQRADGSIIAR
                     IPLSATAAAEIKSLIAREARLRFIKGLTSEDDPAVQIRMRLVPVADVEYDPESGLAIK
                     ARAIAPEVARTVGGEIELRRGDRVMLELMNVGVHDASVSVLYFDSKGRIGALWPHPNY
                     FPGSSGENVIQSATVGRPVWKRIDCPFVIEIAGHDGWVTFKAVATRMQTNFSPLFQPA
                     FSERIMRGGVRGVAAAESPLGLYLMMATTGLTGRARGDNPSGDPSRVQIPQGWGTATV
                     NFHVRSKD"
     gene            complement(10519..14580)
                     /locus_tag="VGB76_10715"
     CDS             complement(10519..14580)
                     /locus_tag="VGB76_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0099409.1"
                     /translation="MTLSPRLNAYLRARRANLLASLALMLLTCASAPAQDDLVYQVRG
                     RVVESPSSETPVGESGASLQTASVIPVPKAEVTLIAEDASVPVASPTSGVSPGVKPDA
                     YSVSTDAEGYFTLPKVKQGNYYIRVKAKDYELDTVRLELYTRKVTEIVIEIDPYGNSF
                     SQVKYRFTQRRKPGSKAIIRALYQRRTLVDARRDGAYPVRTVNQVSLGGASLTRSYDE
                     LAFYLPGIAAPPQTFSNFVGPGFGPGIGTSGQFSANGLRSRANNFTVDGSDNNDEDIG
                     VRRQGFLALVSQPVESIHEYQVITAVAPAQFGRNLGAQVNAVSQFGTSRVNGTLYGTF
                     STSRLNAADFFDTTGGNTTLPLTAQVGSEARPVVVDGRPAFVNNRAGRKDRFTFGQFG
                     GTLGGPVLQSPNEKGWNAFYFFSGEFQGLKATKETHFAVPTVEQRGLLGTGATGLEHD
                     PFTGATLNAHPTAAQGDAIFSFFPFPNNPQGVYDRNTFSQEIPTRGLGYVLSGRLDAD
                     KVLSGRRHQFISRYNHTRDYRDVPAIGGALFSTIRPDVRAQNLSLFVNSLLSSPDSST
                     LLSNQLFLSYGQTRFDFEHRPDTEFLIPSRTNLRSAEERRFLINARLLQNVTLPGDNS
                     VNYVTNPLATAEARLGTPFELLSAPVGQIFIAGFSPVGVDVFTFPQKRTNNTYQISDT
                     VTLQTGNHTAAFGVDVRRSELNSQLPRNSRPLITFNGGAKVRPTTPQGPPFEVTGRFS
                     ALDLAATGAPNGVFQTLTTGDDSTIGLRYYQYNFFVSDEWRVRPNLSLSYGLRYEYNT
                     PPREQHRRIENTFDEPILERVGIARFINPPLGTGESAEVNLALRRSRGIFLPDRNNFA
                     PRLGLAYSFGREDANIIRVGGGIFYDQVPGVVVSQSRNVVPTFITFNTAGIFESQQIA
                     PGRFRSIGLGILLPSTLPIGCGNSGRIPVNTPGTLNTINSQVSVECLLDTYLLSNNGI
                     FGFQPVLQTVLPAAELQMPMAYHYTASFERELGAAETVISVAYVGTQGRHLLRQTTPN
                     LGDNAIIALPAVILTDDRPRFAGFVAQPVQRDAFGELQNRTSRSGGTLRIYEASANSR
                     YDALQLQLRGRFLPRALYQFNYTLSKVTDDASDVFELAGAPALPANSLTRAGERAPAN
                     FDARHIFSGFIVYNLPAFRGGGFARRLFGDWQVTGKTRFQTGQPFTVNTFFDVNADGN
                     LTDRLNTTEGLVRTGDRRQPLLLNADPMSLLAESQRDGVIGRNTFRAGSIFDFDLSFM
                     KTFSFEEEIIVELPDADGNANLADGQEAPKVQTTQRTGKSRQLRLRVDMFNVFNRANF
                     GVPVRYLEAAGFGRAVNTVTPPRRLQFSLRYLF"
     gene            complement(14622..16223)
                     /locus_tag="VGB76_10720"
     CDS             complement(14622..16223)
                     /locus_tag="VGB76_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY0099410.1"
                     /translation="MRILLFLMLWYIICQPNVALSQQNANDIARDTSVYISGITDRKS
                     PLKFYAGSYALVIGISDYKFFGSRENSWSPLSGVKKDVIAIEEALSGAFKVERAEDLD
                     DRDLRNRLAEFIDKYGKNPENRLLIYFAGHGDLLRIDGREVGFIVTKNSPRVRRDPQT
                     NVPVEDYQEFTRRALSMREFRDYVKKIEARHALFVFDSCFSGSVLREMEGSRVASQAV
                     LPGLTGTGLAFLPPEPSVTRPVEPAINVASVLPVRMFITSGTNWQEVQDDSAFRRAFV
                     RAIGRDSQRPADSNRDYYVTGPELWRFLSREVTAREGIAQLPQYGVIDEPERDEGSYV
                     FELPGATKKTIPLDVDGNLWDLSGGQWEVTGRGLHTLGSGILLPKEPHRYSYRDLRIT
                     AVVRLEKDSAAKFVLRAQGASDYYLVEMSGGGSANQALRHKLRAYVVREGRKRELKVP
                     PPSINETNLKKLLSDGKPVKVDIKLYANSIRVQLWDGERPILLVGETVFLIENDIYEF
                     GTVGFLSEGEDRFTVSKFSVESCDH"
BASE COUNT         3041 a         4844 c         5320 g         3236 t
ORIGIN      
        1 tttgtggtta cgcgcgcaag aagttcgccc gccgtcaact gtcaggtgga caggtaagtg
       61 atattcgacg cgagatcgag gtcgtcgctg aactgtgggt tcttgaaggc aaacgtgttc
      121 gcgccgggga agacgaagtc gttcgtcgtg gatagcaccg atttgatcgc gggcaggacc
      181 gcgtcgaggt tggtctgtac gtctccctgt aggccgccga aatccttcac atactgcgtc
      241 atcccgcccc cgaaaccctc gatcactttt tgccaccact tcaagtcgtg cccggccacc
      301 gtaggggggc tgtcgaagtc ggagtcaacg acgacgatgt ccagactgcc gttgtcggcg
      361 aggttcatct gcaaccggag gtcggcatac caggcatagg tggttgcggg catgtccgtc
      421 tcgttgtccg tctccgaaga agagacgatc tccgacgcgc tacaggtgat cgtgccggat
      481 acgtggatgg tgtcgccgtt gaaagtgatc cggcagttcg aactgtagtg ggacgacacg
      541 atggtagtga cgcccgaccc cgtcgtgtgg ctatctttac cactctcctt gtaactgtaa
      601 tcggcgacga cgttgcctga gagcggcggg ttgtgcatgg tgaaggagtt cgtgccgctg
      661 ccctgtccga aggtcacctt gtcgtgtttg gcgttggcgt tcacggtcgg gctgatggtt
      721 tgcaggatgc cgttaaagtg cgggatgaga tagttgagaa tcagatcatg cctgaccgcc
      781 atcgtgccct gaacgctgtt gtcgtccacc cagttgaagc cgaagccgga gggtgggttg
      841 ggcgggagcg ggcgatgatc ggtcatcaac agatagttca gcgtatcgag gccggggttc
      901 gaatggttcc cggtggcatc ggtgtagggc gtgacgcaaa agtcgagggc ggtgggcatg
      961 aaggtcggcg gctgctggtt ggcggcggtg gaatggagcg tgtagccgaa gatggtgccg
     1021 ccctgcgctt gctgttgctt gaggtagact ctcatgatgc cgctcagaag ggtagcggcg
     1081 aggtctggca tattttcgat gttctggaac gtgtcaaagg ccgccgtgtt gaggtcgagg
     1141 tagagttgct ggatggagaa catgtccggg ctgagttggc cgacgtaccg gctgaccgcc
     1201 tgccggacat tcgcgggcag actctccggc gacacctcct tgagcgcgag attgacgaga
     1261 aacccgatga tccacggctc gtttccgctc tgcttcacgt cgaacggagg cacactgatt
     1321 gacttaaatt ccgcattgcc gaaggtgatg ttgtaacgca cggtctgggg ctggccttca
     1381 ttgtaaagct gcacgatgtt gacgggcgcg ccgttctcga cggcgtagcc gagcgtgccg
     1441 gtgaaggtga agacggcggc gtcaggtgtg gtcgggacga agttgccctc tgcgtccgcc
     1501 gtgtaataga gcgcgacgtt cttctgcaac ccgtcgagga aggtcgccag catttcgttg
     1561 atggagttct gcgtcacggc gacgacggta tcgtaagagg tgagttgaat ttgctggatc
     1621 gtcataggct ttagagagtg ggaggtgaag ggtcaaggtc tactcgccgg ggtggactcg
     1681 gcggccagct tcaaggagtc tcgaattgtc atttagcttg cgaggcaagc agggtaatct
     1741 gctgtgagcc gaacatacaa gcgagcgcat gaagctgtca acagaaattt gatgcgccgg
     1801 agctgcgttc tgagcgacaa agatggagaa cggttagcct gcgatggtgt aatatctgcg
     1861 cagaccaagt taccggggtt gcctcgcgca acgtcataga ggagaaggcg ttgactcgga
     1921 acattctcgt tcgaaacaat gtgaaagtct tcggcaaggg cacgcagccc atgctgttcg
     1981 cacacggctt cggctgcgac cagaacatgt ggcgtttcgt gacgcccgcg ttcgaggagc
     2041 actaccgcat cattcttttc gattacgtcg gatcgggaaa gtcagacctt caggcgtaca
     2101 gcccggagcg atacgccacg ctcgaaggct acaccgggga cgtgctcgac atctgcgccg
     2161 aactcgatct gagagatgtc atcttcgtcg ggcactctgt cagcaccgtg gtcggcatgc
     2221 tggcctccat ccgcgaaccg gagcgattcg cacgcctcat cctcgtcggt ccctcgcccc
     2281 gctacatcaa cgacccgccc gcctatgtcg gcggctttga tcgctccgac atcgcggggc
     2341 tgctcgaagt gatggagaag aactacatcg ggtgggcgaa ctacctcgcg cccatcgtga
     2401 tgaagaacgg ggagcggcct gagttgacgc gcgaactcga agagagcttc tgctccacag
     2461 acccgaagat cgcgcgactc ttcgcagagg ccactttcct ctcagacaat cgtgacgacc
     2521 tgccgaaggt gcccgtcccg tcgctcatca tgcagtgctc ggaagactca atcgcgccgc
     2581 tcgaagtggg cgaatacctc cacagacatc tgccgcaaag cacgctgcgc ttgctgcgcg
     2641 ccacgggaca ttgcccgcac atgagccacc cggaggaaac cgttcaggtc atcaaggagt
     2701 atctggccgc gaggtgcgcc gcctgagtgg cctgcgccgc cgtttcgaga agtctgatgg
     2761 acgacctgct gaacactgcg ccgtgcggct tcctgtcatt caacgatgac ggcaagatca
     2821 tgctggtcaa cgccacgctg ctcaacctgc tcgggtatga accggacgaa ctgccggggc
     2881 gacacgtcga atccctcctg acggtcgcca gccgcatctt ctatcagacg catctcttcc
     2941 cgctgctcaa actgcacgac agggttgaag aggtctattt ctcgcttcgc gcgaaggacg
     3001 gggcggagat acccgtgctg gtcaacgccg tgcgccgcga gcgcgggggc gtcttcgcca
     3061 acgagtgcat cctgatgcac atgcgacagc gcaaccgcta cgaggacgag ttgctcaaga
     3121 cgaagcgtgc ggcggaggag gccacgcgcg cgaaggacga attcctcgcc gtggtatcac
     3181 acgaactgcg cacccctttg aatgccatac tcggttgggc gcgaatgttg cagacgggaa
     3241 aactcgacga acggacgggg gtgcgcgcgc tggacgccat cgagcggaac gccgaatcgc
     3301 agaaccaact catcggagac ttgctcgatt tctcgcgcat catctccggc aagatacgcc
     3361 tcgacgttgg ccgcgtcgat ctcgcctccg tcgtccgtgc gtccatcgac gtggtgagtc
     3421 cggcggcaga cgccaaaggg atcagtctgc aagcagacct cgacccggag gctgaggccg
     3481 tctcgggcga ccccgaacgt ctacagcaag tgatgtggaa cctgctgtcg aacgcagtca
     3541 agtttacccc gcagggcggg cgcgtgcagg tacgcctgac gcgcgttgac tccagcgtcg
     3601 agatcgccgt gagcgacacc ggcgaaggta tcgacgcgga gtttctgccc tacgtcttcg
     3661 agcgtttccg gcaggccgac agcgcgacga cgaggcggca gtcaggactc ggtttaggac
     3721 tggcgatcac gaagcacctc ttggagttgc acggcggaac catcacggcg gagagttccg
     3781 gcgaagggca gggttcgacc ttcatcctga gattgcccac catcggcgtc catcaaccgg
     3841 aacgcttctc cataacttct ggagagtggc agtcgcctgc gagcgaagat catacgcagc
     3901 ccgtggaact ggcgaggctc gacggaatcc atgtgctcgt cgtggacgac gaaggagacg
     3961 cgcgtgaact gctaacggcc atcctgacgc aaagcggggc tacggtgacg gtcgcatcca
     4021 acgtcgccga cgccctcgac aagttgcatt cgttgaaacc cgacttgctg ctgagcgaca
     4081 tcgaaatggc gaacgaggac ggctactctt tgattcgcaa ggtgcgcgtt ctggaggagg
     4141 ggcgagggca aagaattccg gccatcgctc tgacggcgca cgcgcgttct tctgaccgct
     4201 cgcgtgcgat ctcgcaaggc tttcagttgc acatctcgaa gccggtcgaa ccagccgagt
     4261 tggtaatcgc catcgtcaac cttctggaca gtgagcgttc ttaggccgcg cgcgttaatc
     4321 gccgtacgag aagatcgtct ctctggactg aaagcaaaaa gcgtcccccg cggagaggac
     4381 gctttcctga tggatagatt ccgcgaccgc ctgcgcctaa cgcaactgcc gcagattata
     4441 gaccgaggcc agagcgttca actcactgcg cacctgcgcc cacaagcgtt ccgtattacc
     4501 tctcaagcga cgattcctga ccgccctgtt aatgtcctcg gccacgttca ggacgctcga
     4561 cacctgcgac ctcgtatcct gatagcgttc cgtgcggtcg aactcttgcc gcagcacgtc
     4621 gagagcgttc tccaagtctc tcgcccgctg gttcaggttg tcctcgcgcc tgctgccgtc
     4681 cgcgcggctg ttgtccagag atcgatcaaa ggagcggacg aacctgtcgc tctggttctc
     4741 cagacgacgg atgatccgct cgacctgccc cttcgaataa cccctgcccc gcgcctgcct
     4801 gccccactgc gcctcggctg gaattgacac cgccgcgacc atgaaaaggg cgatggctgc
     4861 catgataaat ttgacggaaa tgttcttacg catgtttctc tccttttata tgtctttgat
     4921 tttcagggag agagccgcac cccggccatt ctatgaacaa ccgcgcatgg tgcgcccaac
     4981 ccttatgacg caggagaggg gacatttggt tggaattttt ttactgtcgt acgacgttac
     5041 gaattagcag ttttttagtg tcactacggt taaggcgcgc gctctatcgc aggacgtgcg
     5101 aagaatgttc gcgcccgaaa ataatctttg cgccgccgcc cctactcgta acgcagcgcg
     5161 atcagcggat cgactttgac cgcccttaaa gccggaaggt agcaggcgac gagcgccacg
     5221 ccgaagagca gcacggcgat cacgccgaag gtggccgggt ctgtcgcact cacgccgaag
     5281 agcaacccgg acatcaggtg cgtcaggccg agggagagca gcatcccgga caccacgccg
     5341 atcaacgcca gcgtcacgcc gcgcccgagg acgagtttga ggatgtccgt cttctgcgcg
     5401 ccgagcgcca tgcggatgcc gatctcgcgc gtgtgttgcg tgacgttgta ggacataacg
     5461 ccgtagatgc ctacgacggc gagcacgagc gaaagcgccg ccagtacgcc gaggagcgtc
     5521 atgttgagac gacgatcaga aaccgtaccg tcgagaacgg cctgcatcgt ctggacatcg
     5581 aagaggggct gcgccggatc gagcgtctgc acctcgcgcc gcacggcggc tgcgagcgag
     5641 ccggggtcga gacgcgagcg cacggccagc gtcatgttgc gcagtatatc ttcgccgccc
     5701 tgcgcgtagt tgaagtagaa ctcggcggca ggttgttgcg tgagtcccat attcttcacg
     5761 tcggccacca ccccgatgac gggcacccag tcaggccgga tggggcgacc ggagacgcgc
     5821 ttgcccacag ggtcttcacc cggccagatg gtgcgcgcca tcgtctcgtt gatgacgacg
     5881 ccgagcggcg actcctgcgt gtcgcgcgcg tcaaacatgc gaccgcgcaa gagcgccacg
     5941 cccaccgcgc ggaagtagtc agggctgacg acgcgcagtt cgacgagcgg ctcctgtgtc
     6001 ggcgcatagg tcttgccctc gaccgtcatg taaccgttcg tcccgtattg ctcgaccggg
     6061 agatgcgtgg tgagtcctgc cgactcgacg cccggcaagg cagccacgcg ttcgagcacc
     6121 tgccggtgaa aacgcctgag cgcgtcgccg tcggcatatc tctccggcgc cagcgatagc
     6181 ttggccgtca aaacgtgctc cggcttcagc ccgtgatcaa ccgactgcac cttcgcgaag
     6241 ctcttgatca gcagtcccgc gccgatgagc aggacgaagg ccgcagcgat ctccgtaacg
     6301 acgagcgtgt tgcgcatcaa gttgccgccg aagccctgag aggaaccttt gccgccgtcc
     6361 ttgagagcct cctgcacgtt ggtcttgagc gcctgcgcca ccggggcgac gccgaagata
     6421 acgcccgtga ggatggagac gagcagggtg aagccgagca cgcgcaggtc gagcttgatc
     6481 tcactcgaac gcgggagaaa actgaaagcc agcttgctga gtagatcgag tccgagccaa
     6541 gcggttgcga ctccgattgc gccgcccgcg agcgagagca gcacgccttc ggtcaagaac
     6601 tggcggacga gtcgcaggcg gcctgcgccg agcgcgatcc gcacggcgat ctccctgtag
     6661 cgccctgcgg cgcgcgccag cagcaggttc gccacgttcg tgcaggcgat caggagcacg
     6721 aaggcgaccg cgcccatcag catgaacagg ggttggcgca cgtcgcccac gatctcctct
     6781 tcgtacctgc gcaggcgcgc gccccgcccg gcctgcgtct ccgggtactg ctgctccaga
     6841 cgcgccgcga taaggctcat ctgctcgctc gcctgctcca gcgtcacgcc ctgtttcagt
     6901 cgcccgatgg tgaagtaact gcggctgcgc cggtcagtct tctcgccctc cgcgaagacg
     6961 agcggcgacc agagttgcgt gctcgaaagc gcgctcaact cctgcggcat cacgccgacg
     7021 accgtgtact tctgcccgtt gagcgggatc gtcttgttga cgatgttggg gtctgcaccg
     7081 aagccgttgc gccagagcgc gtcgctcaag acgacgacac gctcactgcc cgcttcgtcc
     7141 tcgccatgca gaaacgcgcg gcctagtcgc ggcttaatgc cgagcacgtc gaaatagttt
     7201 gcctgcacct tcaatccttg caggcgttgc gggctatcgc cggactgtag gttaaatgcg
     7261 ccgtccacgt aggcggcgat cccctcgaag accgtgttct gctgctgcca gtcgagcagg
     7321 ttcgggacgg agagcgcgct ctggccggag ggctggaacg tttcgtagag cgcgacgagg
     7381 cggtctgatt cgggaaaggg cagcggacgg agaagcaccg cgttgatcac ggagaagagc
     7441 gccgtgttag cgccgatgcc gagcgcgagc gtcagcacgg cgacgagcgt gaaggcgggc
     7501 tgcttgagca acacacgcgc accgaagcgc aggtcttgcc acaagtctcc gagcaaaggc
     7561 acgccgccct gccgtcggta ttcttctttc gtctgttcga cgccgcccag tttgactaac
     7621 gcctgcctcc gcgcctcctc cggcgacaac cccgccctga tgttgtcctc gacgtgcatc
     7681 tggagatggc tctctaactc ttccgccaac tcgcgttcgc gtgcctcccg accgaagatg
     7741 gcggtcaggc gcacaagcca ggagcgtagc tgtcgaatgt gcatcaagac tcctttgccg
     7801 gggcgaagaa ccgcgccaga atggctgtag tctgttccca ctcgcgggct tctctggcaa
     7861 gctgcctgcg ccctgcgcgg gtcaacttgt aatacttggc cttgcgatta ttgtcggaga
     7921 cgccccactc cgaagagacg tagccctcct gttcgagctt gagcaaggcc gggtagagcg
     7981 ttccgtagtt aatggagagc aggtcgccgc tcgtctgctc gatgcgccgc gcgaggccgt
     8041 aaccgtgctg cgcgcccatc gcttccagcg tcttcagcac catcagggcg agagtgccct
     8101 gccaaatctc cgtcttctct cccatcacga aaacctccta ttggaatggc ataggagcat
     8161 actatttttc cgtatgggaa agcaataggg ggatgaaact gagcaggata gatgctaccg
     8221 ggtggttcta ttcagtcttt cgacctgacg tgaaagttga cggtcgccgt tccccacccc
     8281 tgaggtattt gcacgcgcga cgggtcgccg ctcgggttgt cgccccgtgc cctcccggtc
     8341 aggccggtgg tcgccatcat caaatacaga ccgagcgggg attccgccgc cgcgactccg
     8401 cgcaccccgc cgcgcatgat gcgttcgctg aatgccggtt ggaagagcgg gctaaagttc
     8461 gtctgcatac gcgtggcgac ggctttgaag gtgacccagc cgtcgtgccc cgcgatctcg
     8521 atcacgaacg ggcaatcaat tcgcttccag acagggcggc caacggtcgc gctctggatg
     8581 acgttttcgc ccgaagagcc ggggaagtag ttggggtgcg gccagagcgc cccgatgcgg
     8641 cctttgctgt cgaagtagag cacgctgacc gaggcatcgt ggactcccac gttcatcagt
     8701 tccagcatga cgcggtcgcc gcgacgcagt tcgatctccc cgccgactgt tcgagcgacc
     8761 tccggcgcga ttgcacgcgc cttgatggcg agtcctgatt ccgggtcata ttccacgtcg
     8821 gcgacgggca cgagcctcat cctgatttgc acggcgggat cgtcttcgct cgtgagtccc
     8881 ttgatgaacc tgagtcgtgc ctcccgcgcg atgagcgatt tgatctcggc ggcggcggtg
     8941 gcgctcaagg ggattcgcgc gatgatggag ccgtcagcac gttggagcgt gaagccgcct
     9001 ttgacgcccg cgctcccggc attgccggag tcgctctcgt ccgggcattt gtcccggcag
     9061 atgcgcacgt cccagagatc agggcgcgcg aggttaaagt tcaccagttc caactccctc
     9121 aacctgccga cgaccgctgc cgcttcacca tcgagattga gcgtcgccac tttcaggcgc
     9181 atgtcgccgt aattgtggcg cgtctcgacg gcgcgtgccg cgcgcaactt ttccagcagg
     9241 ctttccacgg gcgcgggctt cactattttc agcaccgacg tgttgagttg aggcgtgacc
     9301 acttcggcct cggccagtgg ctcgctcgtc tctgggtctt tgcccgccgg gtagatggag
     9361 aactgcgacc cggcggtcac gccctgcaag tatccggcag cgaggatgac ctgctccctt
     9421 cgcaccgaaa cttcgacata ctgcttcgac ggcggcaggc tcacgcccga catcaggagc
     9481 gcgtcggtgt cgccttcaag ctgcggcttc tgcgaccaga agggcgactc gctggcgatg
     9541 atgtctgtga tgcgttcgaa gagatcgcgg taagtcgttg ccgcgtcggc cttgcggagc
     9601 gcgcggatga gcgcgtaagt gaacgtgccc atctgcgggt ggttcgcgcc gggtgtagtc
     9661 tccaacgctc tctgatcgtg gcgcgacgcg gcgagcacgg tgagtcccct cgctctcgca
     9721 tcaaccgact ggagcatccc gctcgcactc tcttcgcgcc gcgcgcccgg cgcgacggtg
     9781 ggcgcggctc catgccagcc gcgcccgcgc gccacagtgt tcgccccgcg cgtgttgctg
     9841 ccggagtggc aggcgtccat cgtaacggtg atgctggcgg gcttcttcgc cctcaactcc
     9901 gagaggagtc gtccgaactc gtcgtcgcgg atgtcgtttg aggggtcgca gggcgataca
     9961 tagtcggagg gaacgagggt ttcgtcgagg ccgtcgagtt cgtcgccgac ctgtgggttc
    10021 aggccgtgct tttcgtcgtc gggcacctga ccgccgtggc cggaaaaatg gaagaagatg
    10081 atgtcgccgg gtgccgcctg ctcaatgagg aaggagcgga agacgcccgt gatgttccgg
    10141 cgcgtggttt cctccggcgt acggagcacc ttgatctcgt cgaagccgaa agtttcgcgc
    10201 agcacgagtt caagttgctc aacgtccgtc gcggcgttca gatcaggcca gttcagatcg
    10261 ctgcgcgggc aactactccg cgcgccctcg acgcgttcat aattagagat gccgacgagc
    10321 agagcacgct tgcgcggcgc ggcgtgcggc ctcagtccat gcgcaggact ctgcgccagc
    10381 cgctccgggt tcggaagcgt ggctgtaact atccagacgt gtcccgcgag cgcggcgaag
    10441 ccgaagaggg cggtcagaat ttttcgtatc aagagcatct ccgccggaga gtttttccgg
    10501 cgcacggcga cgcaacctct aaaacaggta tcggagcgaa aactggagcc gccggggcgg
    10561 agtcacggtg ttcaccgccc gaccgaagcc cgccgcttca aggtagcgta cgggcacgcc
    10621 gaagttggcg cggttgaaaa cgttgaacat gtctacgcgt aacctgagtt ggcgcgactt
    10681 acccgtgcgc tgtgtggtct ggactttggg cgcctcctgc ccgtcggcta aattggcgtt
    10741 cccgtctgca tccggcagtt cgacaatgat ctcttcttcg aaagagaagg tcttcataaa
    10801 ggagagatcg aagtcgaaga tgcttccggc gcggaaggtg ttgcgcccga ttacgccatc
    10861 cctctgactt tccgccagca gcgacatggg gtctgcgttg aggaggagag gctgccgcct
    10921 gtcgcccgtg cgcacgagtc cctcggtcgt gttgaggcgg tcggtcaggt tgccgtcggc
    10981 gtttacgtcg aagaaagtgt tgacggtgaa aggctggccg gtctggaatc tggtcttccc
    11041 cgtgacctgc caatcgccga agaggcgacg ggcgaaaccg ccgcctctga aggcggggag
    11101 gttgtagacg atgaagccgg agaagatgtg gcgggcgtcg aaattggccg gagcgcgctc
    11161 gcctgcgcgc gtcagactgt tagcggggag cgcgggcgcg cctgcgagtt cgaacacgtc
    11221 ggaggcgtcg tcggtcactt tcgagagggt atagttgaac tggtagaggg cgcgcggcag
    11281 gaagcgtccg cgcagttgga gttggagcgc gtcatagcgt gagttggccg atgcctcgta
    11341 aatcctcagc gtcccgccgg aacggctggt gcggttttgt agctccccga acgcgtcgcg
    11401 ctggaccggt tgtgcgacga agcccgcgaa gcgcggacga tcatcagtga ggatcacggc
    11461 gggcagcgcg atgatggcgt tgtcgccgag gttgggcgtg gtttgacgca ggaggtgccg
    11521 gccttgcgtt cccacgtagg ccacggagat aaccgtctcg gctgcgccta gttcgcgctc
    11581 gaaagaggcc gtgtagtgat aggccatcgg catctggagt tcggcagccg ggaggacggt
    11641 ttgcaggacg ggttgaaaac cgaagatgcc attgttcgag aggagatagg tgtccagcag
    11701 acactcgacg gaaacctgcg agttgatggt attgagcgtg cccggcgtgt tgacgggaat
    11761 gcgacctgag ttgccgcagc cgatggggag cgtgctcggc agcaggatac ccagcccgat
    11821 gcttctgaac cgccccggcg cgatctgctg actctcgaat atcccggctg tgttgaaggt
    11881 gataaaggtc gggacgacgt tgcgcgactg gctgacgacc acgcccggca cctgatcgta
    11941 aaagatgccg ccgccgacgc gtatgatgtt ggcgtcctct ctgccgaagg agtaggcgag
    12001 gccgaggcgg ggcgcaaagt tgttgcggtc ggggaggaaa atcccccttg agcggcggag
    12061 cgcaagattg acctccgcgc tctcgcccgt gccgagcggc ggattgatga accgcgcgat
    12121 ccctacgcgc tcaaggatcg gctcgtcgaa agtgttctcg atgcggcggt gttgctcgcg
    12181 cggcggcgtg ttgtactcgt agcgcaggcc gtaggagagc gagaggttag ggcgcacgcg
    12241 ccactcgtcc gagacgaaaa aattgtactg gtagtagcgc agcccgatgg tcgaatcgtc
    12301 gccggtcgtc agcgtttgaa agactccgtt gggcgcgccc gtcgcggcga ggtcgagcgc
    12361 ggagaagcga ccggtgactt cgaagggtgg accctgcggg gtcgtcggtc gaacctttgc
    12421 gccgccgttg aaggtgatga gggggcgcga gttgcgcggg agttggctgt tcagttccga
    12481 cctgcgcaca tcgacgccga aggccgccgt gtggttcccg gtctggagcg tgacggtatc
    12541 cgaaatctga taggtgttgt tcgtgcgctt ctgcggaaag gtgaatacgt ctacgccgac
    12601 ggggctgaac ccggcgatga agatttgacc gacgggcgcg ctcagcaact caaacggcgt
    12661 tccgagtctg gcctcggcgg tggcgagagg gttcgtaacg tagttgacgc tgttgtcgcc
    12721 cggcagcgtc acgttctgca agagtcgcgc gttgattaag aacctgcgct cttccgccga
    12781 acggagattg gtgcggctcg gaatgagaaa ctctgtgtcg ggtctgtgct cgaagtcgaa
    12841 gcgcgtctgg ccgtaggaga ggaagagttg attggagagc agcgtcgaag agtcgggact
    12901 gctgagaagg ctgttcacga agagagagag gttttgtgcg cgcacgtctg gacggatggt
    12961 ggagaagagc gcgcctccga tggcgggcac gtcgcggtaa tcgcgcgtgt ggttataacg
    13021 gctgatgaat tgatgacgcc gcccggagag caccttgtct gcgtcgaggc gtccggagag
    13081 gacgtagccc agccccctcg tgggaatctc ctgtgagaag gtgttgcggt cgtagacgcc
    13141 ttgcgggttg ttggggaagg ggaagaagct gaagatcgcg tctccctgcg cggcggtcgg
    13201 gtgcgcgttg agcgtcgcgc cggtaaaggg atcgtgttcg aggccggtcg cgcctgtgcc
    13261 gaggagtccg cgctgctcga ccgtgggcac ggcgaagtga gtctccttcg tcgctttgag
    13321 tccctgaaac tcgccggaga agaagtagaa ggcgttccaa cccttctcgt tggggctttg
    13381 caggactggt ccgccgagcg tgccgccgaa ctgcccgaac gtgaaacggt ctttgcgccc
    13441 ggcgcggttg ttgacgaagg caggccgccc gtcaacgacg acggggcgtg cctcgcttcc
    13501 gacctgcgcg gtcagcggga gggtcgtgtt gccgcccgtc gtgtcgaaga agtctgcggc
    13561 gttgaggcgg ctcgtactga acgtgccgta gagcgtgccg ttcacgcggc tcgtcccgaa
    13621 ctgcgaaacg gcgttgacct gcgcgccgag gttgcgtccg aactgtgccg gggcgaccgc
    13681 agtgatgacc tgatactcgt gaatcgactc cacaggttgc gagacgagcg cgagaaaacc
    13741 ctgccggcgc acgccgatgt cttcgtcgtt attgtcggag ccgtctacgg tgaaattgtt
    13801 ggcgcgcgag cgcaggccgt tggcggagaa ctgaccggag gtgccgatgc cgggtccaaa
    13861 gccggggccg acaaagttgc tgaaagtttg cggcggagcg gcgatgccgg gcaggtagaa
    13921 ggccagttcg tcatagctgc gcgtgagcga agccccgccg agcgaaacct gattcacggt
    13981 acgcacgggg tatgcgccgt cgcgccgcgc atccaccagc gtgcgccgct ggtagagcgc
    14041 cctgatgatc gctttgctgc cgggcttccg ccgctgcgtg aatctatatt tgacctgact
    14101 gaaagagttt ccatacgggt cgatttcgat gacgatctcg gtcaccttac gcgtgtacag
    14161 ttcgagcctc accgtgtcga gttcgtaatc cttggccttc acgcggatgt agtagttgcc
    14221 ctgtttaact ttgggcagcg tgaagtaacc ttcggcgtct gttgaaacgg agtaggcgtc
    14281 gggtttcacg ccgggcgaga cgccggacgt gggcgaagcg accgggacgg aggcatcttc
    14341 ggctatgagg gttacctccg ctttcggaac aggaatgacg ctcgcggttt gcagcgacgc
    14401 ccccgactca cccacggggg tttcggaaga gggggattcg accacccggc cacgcacctg
    14461 atagacgagg tcgtcctgtg ccggtgcgga ggcgcaggtg agaagcatca gggcgagcga
    14521 cgcgagaagg ttagcccgcc gggcgcgaag ataggcgttg agtcttggtg aaagggtcat
    14581 ctcgaaagtt tctcctctgg tgccatgcct gtttgcggag tctaatggtc acaactttcc
    14641 acgctgaact tgctgacggt gaatctgtct tcgccttcgc tgaggaagcc caccgtgccg
    14701 aattcataga tgtcgttttc gatcaggaac acggtttcgc ccacgagcag gatgggacgc
    14761 tcgccatccc ataactgcac gcggatcgag ttggcataca gtttaatatc aaccttcaca
    14821 ggcttaccgt cactcaacaa cttcttgagg ttcgtctcgt tgatcgaagg cggcggcacc
    14881 ttcaactctc tcttccggcc ttcacggacg acgtaagctc gcagtttgtg tcgtagcgcc
    14941 tgattggcag agccgccgcc gctcatctcg acgaggtagt agtcggatgc tccctgcgcg
    15001 cgcaggacaa acttggcggc ggaatctttc tccagcctga cgaccgccgt gatcctgaga
    15061 tcgcgatagg aatagcggtg cggctccttc ggcagaagaa tgccgctccc cagcgtgtgc
    15121 agcccccgcc ccgtaacttc ccactgcccg ccggacaagt cccagaggtt gccatcgaca
    15181 tcgagcggaa tcgttttttt cgtcgcgccg ggtagctcga aaacgtagct gccttcgtcg
    15241 cgctccggct cgtcaatcac gccgtactgc ggcaactgtg cgatgccctc gcgcgcggtt
    15301 acctctctac tcaggaatcg ccacagttcg ggaccagtaa cgtagtagtc gcggttggag
    15361 tcggctggcc tctggctgtc gcggcctatc gccctgacga aggcgcgccg gaacgcgctg
    15421 tcgtcctgta cctcctgcca gttcgtgcct gacgtgatga acatgcgtac gggcagcaca
    15481 gaggcgacgt tgatcgcggg ttcgacgggg cgcgtaaccg agggttctgg cggaaggaag
    15541 gcgaggccgg ttcccgtcaa gcccggaagg acagcctgcg aagcaacgcg cgatccttcc
    15601 atctcacgca gcacgctccc ggagaagcaa ctgtcgaaga cgaaaagcgc gtgtcgcgcc
    15661 tcgatctttt tcacgtagtc gcgaaactct ctcatgctca gcgcgcggcg tgtgaactcc
    15721 tgataatcct cgacgggaac gttggtctgc gggtcgcgcc tgacgcgcgg ggagtttttc
    15781 gtgacgatga aaccaacttc gcgtccgtca atgcggagca ggtcgccatg cccggcgaag
    15841 tagatgagga gacggttttc ggggtttttg ccgtacttat caatgaactc tgccagccgg
    15901 tttctcaagt cccggtcgtc caaatcttcc gcccgctcga ctttaaaggc acccgacaga
    15961 gcctcttcta tcgctatgac atctttcttc actccgctca gagggctcca ggagttctct
    16021 ctgctcccga aaaatttgta gtcgctgatg ccgatgacga gcgcgtaact gcccgcgtaa
    16081 aacttcaagg ggctttttct atcggtgatg cctgagatgt agacggaggt gtcgcgcgca
    16141 atgtcgttcg cgttctgctg cgagagcgcg acattcggtt ggcagatgat gtaccacaac
    16201 atgaggaata gaaggattct cattatcgca gccctctttg agttagagcc tctgctggcg
    16261 cagaacgtga cccggcagga ttgcccggaa gagatgttcc attcatgccg caaccgaata
    16321 cggttgcggg ttaatacgaa gtcgggtcgg gtcggatgtg gcgctctctt cgcggggcga
    16381 gtgtcccgcc ggatatttgc tttacggagt tgtgagaaaa ggccgggaga agcttgtggc
    16441 c
//
LOCUS       DASWNS010000301        10814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_220764, whole genome shotgun sequence.
ACCESSION   DASWNS010000301 DASWNS010000000
VERSION     DASWNS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220764"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..721)
                     /locus_tag="VGB76_10725"
     CDS             complement(242..721)
                     /locus_tag="VGB76_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099411.1"
                     /translation="MRLAPTSLISILASNPCPGEGEGLGDGLGDGLGDAVGLGEGLGD
                     GLAVGLGEGVALGLGDGLGVGVCHALAVEALKANRVRRSMIPAAPLLKFIDCMLFFRR
                     LICSTPSTRKISYPRVSALKNPVRRFLATKETRLITHALFTTVMPNRKTRDNRAFSV"
     gene            690..2135
                     /locus_tag="VGB76_10730"
     CDS             690..2135
                     /locus_tag="VGB76_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099412.1"
                     /translation="MEIRLVGASLNGATPRGEAEFRREAQGSRRLRIKIEDVNLPNGT
                     VLNVLVDNVKVGEIVINSQKGELELRTDHGQTTPPVVNGTSIVISERTGTTIIAGSFR
                     QSLPNPNATPSPTPNPTPGPGGEIRVRIPLNGAGINGMMPQGHADFRQRTDGRRLNVE
                     IQDVNLPAGTTFRVLVNNVSVGQIVLSSFFRGELELNTNDGNSVPVVTNGTTVAVVNV
                     NTGATILAGVFGAIAPAANPLEDGNFFVRQQYLDFLNRAPEQGGLDAWVGVFNRCPNN
                     GYGSTNPECDRVQISSGFYRSPEFSGRGYLIHRLYDAVLGRRPLYAEFMPDMARLGTP
                     QTAAELEASRVAFVTEFMSRPEFNALYGGLANAANAEAFVSKLERTANVRLANHAQLV
                     SEMSSGARTAAQTLRAFIESPEVQNAFFYRGFVTMQYFGYLRRDPEQSGFEAWVNVLT
                     NGNGAVQPGDYRTLIFGFLHSNEYRNRFGQP"
     gene            complement(2296..4116)
                     /locus_tag="VGB76_10735"
     CDS             complement(2296..4116)
                     /locus_tag="VGB76_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY0099413.1"
                     /translation="MKLKLMLALVIGMLSVLLFGGTDSLAFPGFPATTGSAIVNSNRA
                     SYFSDASPESTYSIMATAHAPVSAGSMNAIFTSRAVWEYRPALASYDGGKGSGKWFKV
                     LGGKFEDEGALRFQKQAQRKPRTSRREAHVAAQLDEGLIKYSPENVPGSKLFLDALRL
                     DGLADGAAFSIWPDLSGQGNHATQPEAASRLTYHAAASEDGLPYVAGKENTQLMNVPL
                     PATPTKTMFVVARLTVPFALGYLLKFDTNNHLYSGHNSLGRWGWYKKRGEQGTAPAGY
                     YREWTVLCVRQNGNSIAFFIDGKLVETLTPETAPTAFTQFTLGPGPGSVRQLVMFDRV
                     LTDAEVARHTRWLRKHNRLNYRYEYLDTVISGLSSAALIVRKEGFKGARPLVILNHAA
                     GYEERMYHDLPDFRKLLVALLDEGYIVVASALTDNPATGYASWGNQRSLDANAELYRY
                     VTDHYSVDTNRVAMVGTSMGGLTTLLAFPDGRIPVKGAALYCPVTNLAAQYAYRERMA
                     EGIRAAYDIALDGSNYSAKTEGHDPILRAPSDWTGKRLRFYGGEGDTVVQWSLNAKAF
                     ASRVGGTATEADIVTLDGDHNDNIETTVQDLLAFIRRCLN"
     gene            complement(4433..4660)
                     /locus_tag="VGB76_10740"
     CDS             complement(4433..4660)
                     /locus_tag="VGB76_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099414.1"
                     /translation="MKSYRNAIAVLALTCVFSTSALAGDGILWTEKTPPPPPPPLAGD
                     VIYTDMAAPMPEADIVTETALNVLQNLLTLL"
     gene            4795..6348
                     /locus_tag="VGB76_10745"
     CDS             4795..6348
                     /locus_tag="VGB76_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099415.1"
                     /translation="MALAETILEQLEKPALSRDERARLQCQIAAELEQRGQYDAARDA
                     LGELWSDIGRRPVVEGLSELTTAEVLLRVGSLSGYLGSINQIDGAQNAAQDLIRESIA
                     RFESLGEPTMVATAQCELAFCYRRAGAHSEARLLYNEALNRLTDDNDKELRAKILLRL
                     AVVESFYGRYDDSLRILTDSAELFEESTNDALKGKFHNDLACVLMCLGKAECRPDYTD
                     RAIIEYTAASFHFEQAGHTSYKARAESNLGFLLYTIGHYEKAHEHFNRARDLFVGLGD
                     RGTVAQIDEARARVLLAQGELLGAESAISEAVRVLSQGGEQGALAEALTTQGRVFSKL
                     GNFVESLNALRTAADLAEEAGAVEDAGRALLSLIEEHAGHLAEHELLEAYERANNLLE
                     ETQDAETITRLRSCAIRLVSDRRAALAQRRVSGLEDFWANFNLNEQVHRYEAGYVRQA
                     LIDGEGSITRAARLLGLHHHSTLAAMLEEGGRHKDLAHLRTPPETRRQSIINRNTRRR
                     SPRDKARSI"
     gene            complement(6477..>10814)
                     /locus_tag="VGB76_10750"
     CDS             complement(6477..>10814)
                     /locus_tag="VGB76_10750"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEY0099416.1"
                     /translation="LVGAKNYNEGISILFGDGAGNFLTPTTAPGDASDGIAVGDFNGD
                     GKSDVAALDGNANVSVLHGDGTGQLGAPTLYGVGDWATGIVSADFNADGKPDLAVASR
                     QDAVHLLYGLNGGGFAGTLNFNVGYRPDEIVRADFNRDAYEDLAVINGGTLGTGTFSA
                     QGITILLGDGTGRMGSAPVIPIQSSGTLTALVSADFNNDGKADLAVAAAVHSLDAVEV
                     LLGNGDGTFAAPINIPLNSYGSIAYNLDTADVNHDGFADIVVAFNDSGNFATLLGTGS
                     GHFNVAAGSRVTGSNFFEDFTIADFNQDTHLDLAYVRYHDKFLTILFGNGSGYFNTRR
                     DHALPGNPLTVIAHDFNRDGKADVAFTATSNFNNYYVSARLGDGTGNFGGANDFSVGD
                     YPQAISTGDFNGDNVVDLAVVNSLSASLSVLTGDGAGGFGLSVPFAVGGYPNSSVAGD
                     FNRDGKIDLAVTRQGANSVAILLNDFVAPRPCISVNDVTFTEGDVGAGNASFNVTLSE
                     ASAQTVRVNFRIEPRGLFVLSEMNTFLPTPGVDYQPVSGTLTFAPGTTTQTIDVPVNG
                     DLTDEYDEAFSVLLSSPTSNAVIHDTLGIGTILDNDAPPTFIIQDISASEGNTTFNPK
                     LMEFTVTLSAASGKPILLPYQTADGTATAAVNDYQQAISTLNFAPGTSSRIIGITING
                     DTAYEPDETFFVNLGSPENATLSDAQAQGTIVNDDPQPTISVFPASIGELDSSTGVIN
                     VPVQLSNFSSQPVTVNYTTVDGTANAGSDYVATSGSLTINPGDFSQHIRVTVLGDTID
                     ETDETFSLTLSNPVNATLGMQPSATVTIIDNDGPNISINDISISEGDFGLKNATFNIT
                     LSGASPQSISVNYSTIFTGGSSTSGHDYRPVSQRVFIAPGQTSVPVNVQIIGDTDTEP
                     DETLTVLLSASFGATIADAFGTCIILNDDNAGSVQFSAPSYSVSEGAAFATFDVERVG
                     GSSGTVSVLFNTDGGTATAGSDYQAVGYTITFLHGETAKKGFLVPIKEDTLVEDAETV
                     NLILRNPSNGALLGNPATALLTIRDNDPSMPEAPTLQFGAASYAVGEEEGRVTVQVTL
                     TGGSVGGVSVDYRTVDTDAFTVSCDDPANNNGGAFARCDFATTVGRLDFAAGETQKTI
                     SVPLINDTHDEAAETFQIVLSNASGVAVLGTNSNTTVIVQDDDPLRAANPIFTTSFFV
                     RQHYLDFLSREPEANEPWSAILNNCPDVLNLDAASPSAACDRITVSRSFFGSPEFRLK
                     GFSVFRFYKLVFNRLPEYTEIVSDMSFVAGATAEEVFARKAQLATAFTQRQEFTNAYG
                     GLSNTGYVEALLGRYGLNSVRTNSPQRPDDDALKVILTQADLIGGLNAGTLTRAQVLR
                     AVADSDEVGALEFDNAFVAMQYYGYLRRKPEPAGYEAWLKVLRRGDIRTMVNGFLYSA
                     EYKLRFGRL"
BASE COUNT         2232 a         3156 c         3233 g         2193 t
ORIGIN      
        1 cagagattcc gtttgcagaa ccggacgcgt ctgccgcctt caagcctgtc aaaagtgccc
       61 tttcttctgt atatctcctt cgcatcgcat acttcgttcg cagtttcgac aaaagccctg
      121 tgtttatgcg ggtgaaacga catctgtagg tgctgccggg gaagtggtgc gaattaagta
      181 tcacattttg cagccctgtg cgggctggtg gaatcggctc gataactcga cgagggcgtt
      241 tttagacgct aaaggcgcga ttatcccgtg ttttgcgatt tggcatgacg gttgtaaaga
      301 gtgcgtgcgt gatcaggcgc gtctctttcg tcgcaaggaa gcggcgcacc gggttcttca
      361 acgcagacac gcgagggtac gatatttttc gagtcgaagg ggtagagcaa atgagtcttc
      421 tgaaaaacag catgcaatca ataaatttaa gcaggggcgc ggcggggatc atacttcttc
      481 tcaccctgtt cgccttgagt gcttccacag ccagcgcgtg gcagacgccg acgcccagcc
      541 cgtcaccgag tccaagcgcg acgccttcgc cgagtcctac ggccagcccg tcgccgagtc
      601 cttctccgag tccgaccgca tcgcctagcc cgtcgcctag cccgtcgcct agtccttccc
      661 cctcgccggg acaggggttg gaagcgagaa tggagatcag gctcgtcggc gcgagcctca
      721 acggcgcgac gcctcgcggc gaagccgagt ttcgccgcga agctcaaggc agccgccgtc
      781 tgagaatcaa gatcgaggac gtgaacctgc cgaacggcac cgtgttgaac gtgctcgtag
      841 acaacgtcaa ggtcggggag atcgtgatca actcgcagaa aggtgaactc gaactcagaa
      901 ccgatcacgg gcagacaacg ccgcccgtcg tcaacggcac gagcatcgtg atctccgagc
      961 gcacgggaac aaccatcatc gcaggctctt tcaggcaatc gctgccgaac cccaacgcga
     1021 cgcccagccc gacgcccaac cccacaccgg gacccggcgg cgaaattcgc gtccgcatcc
     1081 cgctcaacgg cgcgggcatc aacggcatga tgccgcaagg ccacgccgat ttccgccagc
     1141 gcacagacgg tcggcgtctg aacgtcgaga ttcaggacgt gaacctgcct gcgggaacga
     1201 cgttccgcgt gctggtcaac aacgtgagcg tcggccagat cgtgctcagc tcattcttcc
     1261 gtggtgaact tgagttgaac actaacgatg gaaacagcgt gcccgtcgtg acgaacggca
     1321 cgacggtggc agtcgtcaac gtcaacacgg gcgcgacgat tctggccggt gtcttcggcg
     1381 cgattgcgcc cgccgccaac ccgctcgaag acggcaactt cttcgtgcgc cagcagtatc
     1441 tcgacttcct caaccgcgcg ccggaacagg gcggactcga tgcgtgggtc ggcgtcttca
     1501 accgctgccc aaacaacggc tacggctcga cgaatcccga atgcgaccgc gtgcagatct
     1561 cttcgggctt ctatcgctcg ccggagttca gcgggcgcgg ctacctcatc catcgtctct
     1621 acgatgcagt gctcggcagg cgtccgctct acgcggagtt catgcccgac atggcgcgtc
     1681 tcggcacgcc gcagacggca gccgagttgg aagccagccg cgtcgctttc gtaacggagt
     1741 tcatgtcgcg gcctgagttc aacgcgctct acggcgggct ggcgaacgcg gcgaacgccg
     1801 aagccttcgt gagcaagttg gagaggacgg cgaacgtgcg ccttgccaat cacgcgcaac
     1861 ttgtctccga gatgagcagc ggcgcacgga cggcggcgca aacgctgcgc gccttcatcg
     1921 aaagtccaga agtgcagaac gcattcttct atcgcggctt cgtcacgatg caatacttcg
     1981 gctacctgcg ccgcgacccc gaacagtcag gcttcgaagc gtgggtcaac gtcttgacga
     2041 acggcaacgg agccgtccaa ccgggcgact accgcacctt gattttcggt ttcctccact
     2101 ccaacgagta tcggaaccgt ttcggtcaac cctaaaccgc acgcggcgca ggcgcgactg
     2161 ttttgtgtca acttaaaaac actaagaggc gttgatctcg aatcaacgcc tctttttttc
     2221 tcttctaacg aactgcccga agccttctct tctctccctg ccgttgcgct ccgtgatatg
     2281 cccggcggcg aacctctaat tgagacagcg ccggatgaag gcgagcaggt cttggacggt
     2341 cgtttcaata ttatcgttat gatctccgtc cagcgtcacg atgtcggcct cggtcgcagt
     2401 tccgccgacg cgtgaggcga acgctttcgc attcaaagac cactgcacga ccgtgtcacc
     2461 ctcccccccg taaaacctca gacgcttgcc cgtccaatcc gacggcgcgc gaagaatagg
     2521 atcgtgtcct tcggtcttcg cggaatagtt cgaaccgtcg agcgcgatgt cgtaagcggc
     2581 gcggatgcct tccgccatgc gctccctgta agcgtactgc gcggcgagat ttgtcaccgg
     2641 acaatataac gccgcgccct tgaccgggat gcgcccgtcc ggaaacgcca gcaacgtcgt
     2701 cagcccgccc atcgaagtgc cgaccatcgc caccctgttc gtatccacgg agtaatggtc
     2761 tgtgacgtac ctgtataact ccgcgttggc gtcgagcgac cgctggtttc cccaactcgc
     2821 gtagcctgtc gccggattgt ctgtcagcgc ggaagcgacg acgatgtagc cttcgtcgag
     2881 gagcgcgacg agcagtttgc ggaagtccgg cagatcgtga tacatgcgct cctcgtaacc
     2941 ggcggcgtgg ttcaagatca cgagcgggcg cgccccctta aaaccctcct tgcggacgat
     3001 caaggccgca gacgacaagc cgctgatcac cgtatcgaga tactcgtagc gatagttgag
     3061 acggttgtgc tttcgcagcc agcgcgtgtg acgtgcgact tccgcgtccg tcaacactcg
     3121 atcaaacatg acgagttgac gaacgctccc cggccccggt cccaatgtga actgcgtgaa
     3181 ggccgtggga gccgtctccg gcgtcagcgt ctccacgagt tttccgtcaa tgaaaaaagc
     3241 gatgctgttg ccgttctgcc tgacgcacaa aaccgtccac tcgcggtaat agcccgccgg
     3301 tgccgtgccc tgctcgcccc tctttttata ccagccccag cgaccgagcg agttgtgccc
     3361 cgaatacaga tgattgttcg tgtcgaactt caacagataa cccaacgcga acggcacggt
     3421 caggcgcgca acgacgaaca tcgtcttcgt cggcgtcgcc gggagcggga cattcatcaa
     3481 ctgcgtattc tctttgcccg ccacgtaagg caagccgtcc tccgacgctg ccgcatgata
     3541 ggtgagacgc gacgcggctt cgggctgcgt ggcatggttg ccctgcccgc tcaagtcagg
     3601 ccagatggag aaggctgcac cgtcggcgag cccgtccaga cggagcgcgt cgagaaagag
     3661 tttagagccg ggtacgttct cagggctgta cttgattaaa ccctcgtcaa gctgggcggc
     3721 gacgtgagcc tctcgcctcg acgttctcgg ttttctttgc gcctgcttct gaaacctgag
     3781 cgcaccctca tcctcaaatt ttccacccaa caccttgaac catttgccac ttcccttgcc
     3841 cccgtcataa gaagccaacg caggccggta ttcccacacc gcgcgactcg taaagatcgc
     3901 attcatactc ccggcggaaa ccggagcgtg cgccgttgcc ataatcgaat aggtggattc
     3961 cggagacgcg tctgaaaagt aggatgctct atttgaattg acgatggcgg aaccggtcgt
     4021 agccgggaaa ccgggaaacg ctaaactatc cgtcccgccg aacaacaaga ccgacagcat
     4081 tcctatcaca agggcgagca tgagctttaa tttcatattg cgatgtcttg tataaaccgg
     4141 cggggggaaa tccggttgag agcttgtggt tcgaggatgc aattatcaat gtctcctgtg
     4201 gcgtgaatta ggattgtgcg ggcatccccg gctcagtcgc gcgcattata cacccgccgt
     4261 gactttcaac ccgtacaagt taagacttaa cttaataatt aaccttgcca cattgaacag
     4321 aaattttctc ccaccgcccc gcaattgttg tatattcccg ccagacacac gaaggccgct
     4381 ccgcccctga gcctcagggg cagcaaaaaa aaacttaaac tgttctagct acttagagca
     4441 gcgtcagtaa attttgcaaa acgttgagcg cagtctccgt cacgatatct gcttcgggca
     4501 tgggagcagc catatctgta taaattacgt cgccggctag cggaggcggc ggaggtggcg
     4561 gagtcttctc tgtccagagt atgccgtccc cggcgagggc tgacgtagag aaaacacatg
     4621 ttaaggccag tacggcaata gcattgcgat agcttttcat ctcttctttt ccttttgagg
     4681 tagttagtga agccagtttc ttctggtttt gatttgcgtc ctatccgact gataggtcgt
     4741 gactttagcc tgtctgaaaa attcttagca agcacaaaga aagcgattca tcacatggct
     4801 ttagctgaaa ccatacttga acagttggaa aaacctgcgt tgagccgcga cgagcgggcg
     4861 cgacttcaat gccagattgc ggcggagttg gagcagagag gccaatatga tgccgcgcgc
     4921 gacgcgcttg gcgaattgtg gtcggatatt ggccgacgcc ccgtggtcga aggactctcc
     4981 gaattgacga ccgcagaggt acttctgcgc gtcggctcac tctccggtta cctgggaagc
     5041 atcaatcaga ttgatggagc gcagaacgcg gcgcaagact taatccgcga aagtatagcc
     5101 cgttttgaat ctctgggaga gccgacaatg gtagccacgg cacagtgtga gcttgctttc
     5161 tgctaccgtc gcgccggtgc acatagcgaa gcgcggttac tgtacaacga ggcactgaac
     5221 cggctgactg acgataacga taaagagttg cgggctaaaa tacttctacg tcttgcagtc
     5281 gtcgaatcat tttatggccg ttatgacgac tcacttcgca tcctgacaga ttcggcagag
     5341 ttattcgagg agagcaccaa cgatgcgctg aaaggaaaat ttcataacga cctcgcctgc
     5401 gtactcatgt gtctgggcaa agccgaatgc cgcccggatt acacagaccg cgccatcatc
     5461 gaatataccg ccgcatcatt ccacttcgaa caagcaggtc atacgagcta taaggcgcgt
     5521 gcggaaagta atttaggatt cctgctctac accatcgggc attacgagaa agcccatgag
     5581 cacttcaatc gtgcgcgcga cctgttcgtc ggcttggggg ataggggcac ggtggcgcag
     5641 attgatgaag ctcgcgcacg cgtcctgctg gcacaaggcg aactacttgg agccgagagc
     5701 gcgataagcg aggccgtgcg cgtgctctcg caaggcggcg aacagggcgc tctagccgaa
     5761 gccctcacga cacaaggccg tgtcttctcc aagttgggca acttcgtcga atcgctcaac
     5821 gcgttgagaa ctgccgccga cctcgcggag gaggccgggg cggtcgaaga tgccggacgc
     5881 gcgctgctct cgttgatcga ggaacacgcc ggccatctgg cagagcacga actgctggag
     5941 gcatacgagc gagcgaataa cttactcgaa gagacgcagg acgccgagac gattacgcgt
     6001 ctgcgctctt gcgccattcg tctcgtgtct gaccgccgcg cggctttggc acagcggcgg
     6061 gttagcggcc ttgaagattt ttgggcgaac ttcaatctca acgagcaggt acacagatac
     6121 gaggccggat acgtgcgtca ggctttgata gacggtgaag ggagcatcac gcgcgccgca
     6181 cgtctgctgg gactgcacca tcactccacg ctcgcggcga tgctcgaaga aggaggcagg
     6241 cataaagacc tcgcccacct gcgcacgccg cccgaaacgc gcaggcagag catcattaac
     6301 cgcaacacac gccgtcgtag cccccgtgat aaagcacgct ccatctaata ttctctgcgt
     6361 cgaagattaa caggtggcgg ccttcctgtg tatcgtgacg agaagaggcc gtccgcgaac
     6421 gctaacgcgg acggcctctt cttgcgttcc gactctggtt ccgactccgg cagtctttat
     6481 aagcgaccga agcggagttt gtactcggcg gagtatagaa acccgtttac catcgtgcgg
     6541 atgtctccgc gccgcaacac tttgagccac gcttcgtagc ccgcaggctc cggcttgcgc
     6601 cgcaggtaac cgtaatactg catcgccacg aacgcgttgt cgaactccaa ggctccgacc
     6661 tcgtccgagt cggccactgc gcgcagcact tgcgcacgcg tcagcgttcc ggcattgagg
     6721 ccgccgatta agtcagcctg cgtcaaaatc accttcagtg cgtcgtcgtc aggccgctgt
     6781 ggggagttcg tcctgacact gttgagtccg taccgcccca agagtgcttc cacatagccc
     6841 gtgttcgaca ggccgccgta tgcgttcgtg aactcctgcc gctgtgtgaa ggctgtcgcc
     6901 agttgcgcct tgcgggcgaa aacttcttcc gccgtcgcgc ccgccacgaa gctcatgtcc
     6961 gagacgatct ccgtatactc cggtagacga ttgaagacca gtttgtagaa gcggaagacg
     7021 gagaagcctt ttaagcgaaa ctcgggcgag ccgaagaacg accgcgagac ggtgatgcga
     7081 tcacacgccg ccgaagggct ggcggcatcg aggttgagca cgtcaggaca attgttcaag
     7141 atggccgacc agggttcgtt cgcttccggc tcgcgcgaaa ggaaatcgag atagtgctgt
     7201 cgcacgaaga aggatgtcgt aaagatcgga ttcgccgccc tcaaaggatc gtcatcctgc
     7261 acgatgaccg tggtgtttga gtttgtgccg agaacggcga cgcccgacgc gttcgagagg
     7321 acgatctgga aagtctcggc ggcctcgtcg tgagtgtcgt tgatgagggg gacagagatc
     7381 gtcttctgcg tctcacccgc agcgaagtcg aggcgaccga ccgtggtggc gaagtcgcag
     7441 cgagcgaagg ctccgccgtt gttgttcgcg gggtcgtcgc aagagactgt gaaggcgtct
     7501 gtatccaccg tccggtagtc taccgacacg ccgcccacac tcccgcctgt cagcgtcacc
     7561 tgcaccgtca cacgcccttc ctcctcgccc accgcgtaac tcgccgcgcc gaactggagg
     7621 gtcggggctt ccggcatcga gggatcgtta tctcgaatgg ttaacaacgc cgtcgcgggg
     7681 ttgccgagga gcgcgccgtt cgacggattg cgcaggatga gattgaccgt ctccgcgtct
     7741 tcgacgagtg tgtcttcttt gatcgggaca aggaagccct tcttggctgt ctcgccgtga
     7801 aggaaggtga tggtatagcc gactgcctga tagtcgctgc ccgcagtggc cgtgccgccg
     7861 tcggtgttga agaggacgga gacggttccg ctgctcccgc ccacgcgctc tacgtcgaag
     7921 gtggcgaagg ccgcgccctc gctgacgctg taggacggcg cgctgaactg cacgctgccc
     7981 gcgttgtcat cgttgagaat gatgcaggtg ccgaaagcgt ccgcaatcgt cgctccgaaa
     8041 gaggcggaga gaagaacggt gagagtctcg tccggttcgg tgtcggtgtc gccgataatt
     8101 tggacattca ccggcaccga agtttggccg ggggcgataa acacgcgttg gctgacgggg
     8161 cggtagtcat ggccgctagt gctggagccg ccggtaaaga tggtcgaata atttacgctg
     8221 atggattgcg ggctggcccc cgagagcgtg atgttgaacg tcgcgttctt caacccgaaa
     8281 tcgccttcgc ttatgctgat gtcgttgatc gagatattcg gcccgtcgtt atcaatgatc
     8341 gtgacagtag cagacggctg catgccgagc gtggcattga ccgggttgct caaggtaagg
     8401 ctgaacgtct cgtcagtctc gtcaatcgtg tcgccgagga cggtcactcg gatatgttgg
     8461 ctgaagtcgc cggggttgat ggttaaggaa ccggaggtgg cgacataatc gctgcccgcg
     8521 ttggccgtcc cgtctaccgt cgtgtagttg acggtgacgg gctgagatga aaaattggac
     8581 aactgcacgg gaacgttaat cacgcccgtg ctcgaatcca gttctccgat ggaggctgga
     8641 aagacggaga tggtgggctg cggatcgtcg ttgacgatag ttccctgcgc ctgcgcatcg
     8701 ctcagggtcg cgttttccgg gctgcctaaa ttgacgaaga aagtctcgtc cggctcatag
     8761 gccgtgtcgc cgttgatcgt gatgccgatg atccttgagg aagtgcccgg agcgaagttc
     8821 agcgtactga tggcctgctg gtaatcgttc accgctgcgg tagccgtgcc gtcggcggtc
     8881 tggtatggca agaggatagg cttgccgctc gccgccgaga gcgtgacggt aaactccatc
     8941 agcttcggat tgaaggtcgt attgccttca ctggcggaga tgtcctgaat gatgaaggtg
     9001 ggaggcgcgt cgttatccag gatcgtgccg ataccgagcg tgtcgtggat gacggcgttc
     9061 gacgtgggcg aagagaggag cacggagaag gcttcatcat attcgtctgt gaggtcgccg
     9121 ttgacgggaa cgtcgattgt ctgcgtcgtc gtccccggcg cgaaggtgag cgtgccggaa
     9181 accggctggt aatctacgcc cggcgtgggc aggaaggtgt tcatctccga caacacgaat
     9241 agaccgcgcg gctcgatgcg gaagtttact ctcaccgtct gagcggaagc ttcggagagc
     9301 gtgacgttga aagacgcgtt gcctgcaccc acgtcgccct ccgtgaacgt cacgtcgttc
     9361 acggagatgc acggacgcgg cgcgacgaaa tcgttcagca agatcgcgac gctgtttgcg
     9421 ccctgccgcg tcacggcgag atcaattttg ccgtcgcggt tgaaatcacc tgcgacggac
     9481 gaattcggat aacctccgac agcgaaaggc acggagaggc cgaacccgcc cgcgccgtcg
     9541 cccgtcagga cagacaagct cgcgctcagg ctgttgacga cggcgagatc gacgacattg
     9601 tcgccgttga aatcccctgt ggagatggcc tgcggataat cgccgacgct gaaatcgttt
     9661 gcgccgccga agttgcccgt cccgtcgccg agcctggccg agacataata attgttgaag
     9721 tttgaagtgg cggtaaacgc cacgtcggcc ttgccgtcgc ggttgaagtc gtgagcgata
     9781 acggtgagcg ggttgccggg cagcgcgtga tcgcgcctcg tgttgaagta gccgctgccg
     9841 ttgccgaaca gaatggtcag aaatttatcg tgatagcgaa cgtaggcgag atcgagatga
     9901 gtatcctgat taaagtcggc gatggtaaag tcttcgaaaa aattgcttcc ggtgactctg
     9961 gaaccggcgg cgacattgaa atggccggag ccggtgccta acaacgtcgc gaaatttcct
    10021 gagtcattga aggcgacgac gatgtcggca aagccgtcgt ggttaacgtc agcggtgtcg
    10081 aggttgtagg cgatgcttcc gtaggaattg agcgggatgt tgatcggggc ggcgaaagtg
    10141 ccgtcgccgt tgccgagcaa cacttcaact gcatccagag agtggacggc tgcagcgacg
    10201 gccagatcag ctttgccgtc gttgttgaaa tcagccgaga cgagggcggt gagagtgccg
    10261 ctgctctgaa ttggaattac cggagcagac cccatccttc cagtgccgtc gccgagcagg
    10321 atcgtaatcc cctgcgcgct gaacgtcccg gttccaagcg tgcccccgtt aataaccgcg
    10381 aggtcttcat acgcgtcgcg attgaaatcg gcgcggacga tctcgtcggg tctgtagccg
    10441 acattgaaat tgagcgtgcc cgcaaatcca cccccgttca gaccgtagag caggtggacg
    10501 gcgtcctgcc gacttgcgac cgcgaggtcg ggtttgccgt cggcattgaa atctgcggag
    10561 acgatgcccg tcgcccagtc gccgacgccg tacagggtcg gcgcgccgag ttggcctgtg
    10621 ccgtcgccgt gcaggacaga gacgttggcg ttgccgtcaa gcgcggccac atcgctcttc
    10681 ccgtcgccgt tgaaatcgcc gaccgcgatt ccgtcgctgg catcgccggg agcagttgtc
    10741 ggagtgagga aattgcccgc gccatcgccg aacagaatgg agatgccttc gttgtagttt
    10801 tttgccccga cgag
//
LOCUS       DASWNS010000302         7939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_245709, whole genome shotgun sequence.
ACCESSION   DASWNS010000302 DASWNS010000000
VERSION     DASWNS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245709"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VGB76_10755"
     CDS             <1..475
                     /locus_tag="VGB76_10755"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEY0099417.1"
                     /translation="RAWLVGEAEALDGEEALRRIRGESLTAFDPRRTALLEVKPEELP
                     KLTGAALPPEATARVVEYQPSRLVIETSAPAAAVLVVSEIIYPGWEATVDGVAARIDT
                     TNYLLRGVAVPAGVHRVEMRYTAPAARVGAVISACALLLLCGLALYDWRAGRSGD"
     gene            683..2881
                     /locus_tag="VGB76_10760"
     CDS             683..2881
                     /locus_tag="VGB76_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099418.1"
                     /translation="MAETTRTRGASVEFARALFAFVQERAFVRECLIFLAFVALTALM
                     SWPWVRHPLDTVSDLGDPYNIAYTLWWDYHQTFHDPRHLFDATIFYPYRDTLAFSEHD
                     YGIALLFFPLFALGVRPLTVHSLATFLAFVLSGYGAFRLARTLTASYGAAWVAGVAFA
                     FIPYRFQRLPHLHYIFAGWIPLLFEALILFVRGRSWRSAAWLGTAFLMNALTCISWFI
                     LTLIPLGLSGAFLVVWYGARREGRALWLRGAVALGAASLALLPFLLPYRRVAALYGFV
                     RDAKEVKMYSATALNWLAASPRLKLWEGFGGVAATEEMSLFPGLLLPLLAFAALILYR
                     TRTRDDANDDTHHATRAGRHADTRADVHDDMHKDEHNNARTDTRRAGSARWRRFAAPS
                     ALALLLLDAFALGAGLVALIISAYGTYDFRIFNWHLVTATRPEAAFIACVAAVVARAG
                     VTFWPVLRGIVARRFGVREDADRSFETFVLGAICLSIGFVGSLGLHAFFHRFLFEHFE
                     VFKSQRIPARWAMICYVGLALLAGLGARALAKAFAGRRRSSARRAGVYALIVVALLFE
                     LRVAPLVVTRGEAYPDELTLRLKETPMRGGLVELPALGAAIFEHMIRAADHGKPIVTA
                     ASSFLPPIMRRVEAMSQQKPVPPEFLDLLESIPASYLVIHYSHMTPEETTATETLLRH
                     GLQTGRLRFIRSYQDRGRKDLYAITRTEPAAQGESPAPPELAAPAGDGLK"
     gene            2961..5573
                     /locus_tag="VGB76_10765"
     CDS             2961..5573
                     /locus_tag="VGB76_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099419.1"
                     /translation="MKSLRYMTTGRLAARRGADAAAMLCIAGFFALFFGWILFGDKYV
                     VGGDAFAYSYPLRHAAWEMIRQGQLPLWTPHVLSGYPLLSMAQLGLGYPLTWSYLFLD
                     APRAEQFYILAPFLLAPAFTYAYLRELGRSRTAALLAGLSFTYGGATTGLLGLIGFPN
                     HSLMWLPLMLIAVERARRTDARFAACLPLAAGAYAMSVLNGYAQGFLYLGLVALAYAI
                     FLSLFAATRGASAGGDEESDAAHLHVWRRRWRRWRPAAVALCGIALGAGIGAFQILET
                     MRAARRSIRSTLIYPLFVSGSFSPRVAFESLVAPLYTGRFADVGTYVAPLSLLLALAG
                     ILSTFRSKTRLERQREGQREAQRDVRVYFWTATALVSCVFILGHHTPVYGLLYHVPVV
                     NSFRVPSRFSYIWTFAAAVLAAYGWDALPLLLRRARATTGAPTTATGTRSLHARWHIT
                     LVATLSVLSALVALLWWRRIVTSAVTDLDAATGVPRVWVEGFWYAGLPVSEYLAWKAA
                     FTFLTILLIWRGWKIDAARTRGFVLACALALVCFVEPFFVVAQWWQPFAKSHARFNTA
                     APVTRLLQAREGQAGRATLPRSRIYTRVNLFAEEFTESPRFDPPNLTALYGLENVGGY
                     EPLLLERYSRALGNVNLDAASPLPGSAPNRALFDARSHVLDLLGAKFVVNVNMSKADA
                     PPVETDLDAQRWAVIHDADNVRVLENRRALPRAWLVGEAASVDGEEALRRISGAGDRE
                     FDPRRTALLEVAPPEMPALAGGELAPESTEARITLYEPNRLVVETRAAQPSALVVAEI
                     FYPGWEATIDGTPARIHLTNFIMRGVAVPAGAHRVEMRYTAPAARNGAIISALTLLLL
                     IVLAIRGRTRGGAA"
     gene            5627..>7939
                     /locus_tag="VGB76_10770"
     CDS             5627..>7939
                     /locus_tag="VGB76_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099420.1"
                     /translation="MFLEQAQVSRRGTKAQRRDDADFETRAYSLNVKIPETSKGVWRR
                     RCAARRGDAAAMLCIVAFFCLFFGWAIFRGHFLIGGDVFFYTYPLRSVAWEMIRQGQL
                     PLWTPHVLSGYPLLSMAQLGLGYPLTWAHLFLPARHAELIYVLAPFLLAPAFTYAYLR
                     ELGRSRTAALLAGLSFAYGGLMTNTYGMNAIPTNALMWLPLLLIAIERVRTRGLARSV
                     AAAALPCAMSVLTGHGQSFLFVCLLATAYALFLGFAYDAGDAQENRREHEPESEAGHG
                     RARRRAWFGWQRWSPLLASTGGMLLAAGIAAFQILETMRAARRSIRSNLSYEFFVEGS
                     FTPLAAWRSFVAPLYHFIEVTTYISPLAFACAVCAVAFNLRRGRRQTPHRAALTIDVQ
                     AGTDARVFFWAATALVAVLLMLGGNTPLYRALHQVPVFNLFRRPSRHVFEWTFALSVL
                     AAYGWDALDSFSRRGVSGARGGVERGKLVVATLSLLIAAALGVVWWQTAHGLPYVGDA
                     VPRAESIYLKWKILFTLALAVACGFALRLSAARRRRAWLLACVVLLACFVESHLLVTH
                     WWPGTIKTTERLTTPARATRYLQQFPAHENRVYVRANSGEEESAAAPRFDALNLTARY
                     GLHNVAGYEALLFERYSRALGDVDYDAVNPRRGARATLALFAPRSHVLDLLNTTRVVT
                     FANLATTPTVEARGAEADGDALDATRWQREEEFDGVLVLRNERALPRAWLVGEAEALD
                     GEEALRRIRGESLTAFDPRRTALLEVKPEEL"
BASE COUNT         1178 a         2695 c         2488 g         1578 t
ORIGIN      
        1 gcgagcgtgg ctggtgggag aagccgaagc gttggacggc gaagaagccc tgcgccggat
       61 tcgcggcgaa agcctcaccg cgttcgaccc gcgccgcacg gctctgctgg aagtgaaacc
      121 ggaagagttg cccaagctca ctggcgcagc gttgccgcca gaagcgacgg cgcgcgtggt
      181 tgaataccaa ccgtcacggt tggtcatcga aacgagcgcg cctgcggcgg ctgtgctggt
      241 cgttagcgag atcatctatc cgggatggga ggcaacggtg gacggggtgg cagcgcgaat
      301 tgatacgacc aattatctgt tgcgcggcgt ggccgtgcct gcgggcgtac atcgcgtgga
      361 gatgcgctac acggctcctg ccgcgcgcgt cggcgcggtc atctcggcct gcgccctgct
      421 gctcttatgc ggcctcgcgc tttacgattg gcgcgccgga cgaagcggag attgataatt
      481 cggagcgcgt ttcaagaccc gcccatgctt tccgtgcgcc ttcgcgcccc ggcgtgggtt
      541 atcttctttc gcaaagataa gtttcgcgct tgacgcgtag ggaagaagtc gcgcggaagc
      601 gtaggtttcg aaagacaccc cggcagcgtt ttaactcaaa ggctcgacga gacgatcaat
      661 ggactcaaag aagcatcgcc gcatggcaga gacgacgaga acgcggggag cgagcgtgga
      721 gtttgcgcgc gctcttttcg cctttgtgca ggagcgtgct ttcgtgcgcg agtgtctcat
      781 ctttctcgcc ttcgttgctt tgacggcgtt gatgtcgtgg ccttgggtgc gtcatcccct
      841 ggacacagtc tccgatctcg gtgaccccta caacatcgct tacacgctct ggtgggacta
      901 ccatcaaacc tttcacgacc cgcgacacct cttcgacgcg accatctttt atccttaccg
      961 cgacacgctc gccttctccg aacacgacta cggcatcgcg ctcctcttct tccccctctt
     1021 cgccctcggc gtgcgcccgc tcacggtgca tagcctcgcc acctttctgg ccttcgtcct
     1081 ctcaggctac ggcgcgtttc gtctcgcgcg cacgctcaca gcctcttacg gcgcggcgtg
     1141 ggtggcgggc gtggccttcg ccttcatccc gtatcgcttt caacgcctgc cgcacctgca
     1201 ctacatcttc gccgggtgga ttccgctgct ctttgaagcg ttgatacttt tcgtgcgcgg
     1261 gcgtagctgg cgcagcgcgg catggctcgg caccgccttt ttgatgaacg ctttgacgtg
     1321 catcagttgg tttatcctga cgctcatccc gctcggattg tcaggcgcgt ttctcgtcgt
     1381 ctggtacggc gcgaggcgcg aggggcgcgc gctgtggctg cgcggcgcgg tcgccctcgg
     1441 cgcggcctcg ctcgcgttgc tcccgttcct gctgccctac cggcgcgtgg ccgcgctcta
     1501 cggtttcgtg cgcgacgcga aggaagtaaa gatgtattcg gcgaccgcct tgaactggct
     1561 cgccgccagc ccgcgcctca agctgtggga aggcttcggc ggcgtcgcgg cgacagagga
     1621 gatgtcgctc ttccccggcc ttctcctccc gctcctcgcc ttcgccgccc tcatcctcta
     1681 tcgcacgcgc acacgcgacg acgcgaacga cgacacgcat cacgccacgc gcgccggtag
     1741 acatgctgac acgcgcgccg acgtgcacga cgacatgcac aaggacgagc ataacaacgc
     1801 gcgcaccgac acgcgcaggg cggggagcgc gcgttggcga cgcttcgctg ctccatcagc
     1861 actcgcgttg cttcttctcg acgccttcgc cctcggcgcg ggactcgtcg cgctcataat
     1921 ctccgcttac ggaacttacg acttccgaat tttcaactgg catctcgtca cggctacccg
     1981 accggaagcc gctttcatcg cgtgcgtggc ggcggtcgtc gcgcgggcgg gcgtcacctt
     2041 ctggccggtg ttgcgtggga tcgtcgcgcg gaggttcggc gtcagagagg acgcggatcg
     2101 aagtttcgaa acgtttgtgc tcggcgcgat ctgtttgagc atcggcttcg tcggctcgct
     2161 cggcctgcac gccttttttc atcgctttct cttcgaacac ttcgaggtct tcaaaagcca
     2221 gcgcatcccc gcgcgctggg cgatgatctg ttacgtcggc cttgcgctgc tcgccggact
     2281 cggcgcgcgc gcgctggcga aagcgttcgc cgggcggcga cgttcaagcg cgcggcgggc
     2341 tggcgtctac gctctcatcg tcgtcgcgct cctgttcgag ttacgcgtcg cgcccctcgt
     2401 cgtcacgcgc ggcgaagcct acccggacga actcaccctg cgcttgaaag agacgccgat
     2461 gcgcggcggc ctcgtcgaac ttcccgcgct cggcgcggcc atcttcgaac acatgattcg
     2521 cgccgccgat cacggcaagc ccatcgtcac cgccgcttcg agtttcctgc cgccgatcat
     2581 gcggcgcgtc gaagcgatgt cgcagcagaa acccgtgccc cctgagtttc tcgatctgct
     2641 tgagtccatc cccgcctctt atctcgtcat acactattcg cacatgacgc ccgaagagac
     2701 gacggccaca gagacgctgc tccggcacgg acttcaaacc ggacgcctgc gcttcatacg
     2761 cagttatcag gatcgcgggc gcaaagacct ctacgcgata acccgtacgg aacccgccgc
     2821 gcagggcgaa tcgcccgcgc ctcccgaact cgccgcaccc gccggagacg gcttgaagtg
     2881 aattgcagaa gaggtgactc tgacaggcgc gtgcgcgcgt gaacttcaga gacggacgcc
     2941 gcgcatcgct cgaaacgtag atgaagagtt tgagatacat gacgacgggg cggttggcgg
     3001 cgcggcgcgg cgcggacgcg gcggctatgc tctgcatcgc cggtttcttc gcgctcttct
     3061 tcggttggat cttgttcggc gacaagtacg tcgtcggcgg cgatgccttc gcttattcct
     3121 acccgctgcg ccatgcggcc tgggagatga tcaggcaggg gcaactgccg ctctggacgc
     3181 cgcatgtgct ctccggctac ccgctccttt cgatggcgca actcggcctc ggctacccgc
     3241 ttacgtggag ttacctgttt ctcgacgccc cgcgcgccga acagttttac atcctcgcgc
     3301 cgttcctgct cgcgcccgcg ttcacctacg cctacctgcg cgaactcgga cgcagccgca
     3361 ccgccgccct cctcgccgga ctctctttca cctacggcgg cgcgacgacc gggctgctcg
     3421 gcctcatcgg atttcccaac cactcgttga tgtggctgcc gctgatgttg atcgccgtcg
     3481 agcgcgcacg acgcacggac gcgcgcttcg ccgcctgcct gccgctcgcg gcgggcgcgt
     3541 acgccatgtc ggttctgaac ggctacgcgc agggttttct ctacctcgga ctcgtcgcgc
     3601 tcgcctatgc catcttcctt tcgctgttcg cagcgactcg cggcgcgagc gcgggcgggg
     3661 acgaggaatc ggacgcggca catctacacg tctggcgacg acgctggcga cgctggcgtc
     3721 cggcggcggt cgcgctctgc gggatcgcgc tcggcgcggg catcggcgca tttcaaattc
     3781 tggagacgat gcgcgccgcg cgtcgcagca tccgcagcac gctcatctac ccgcttttcg
     3841 tgagcgggtc gttttcgccg cgcgtcgctt tcgagtcgct cgtcgcgccg ctctacacag
     3901 ggcgtttcgc cgatgtcgga acttacgtcg cgccgctctc cctcttgctc gcgctcgcgg
     3961 gcatcctctc cacgttcaga agcaagacgc gacttgaacg gcaacgcgaa gggcaacgtg
     4021 aagcgcagcg cgacgtgcgc gtctacttct ggacggcgac cgcgctcgtc tcctgcgtct
     4081 tcatcctcgg ccatcacacg cccgtctatg gcttgctcta tcacgtcccc gtcgtcaaca
     4141 gttttcgcgt gccctcacgc ttctcctaca tctggacttt tgccgccgcc gtgctcgccg
     4201 cctacggttg ggacgcgctc cctctactgc tccggcgcgc acgcgcgacg acaggtgcgc
     4261 ccacgacggc gacgggcacg cgctcgttac acgcgcgttg gcacatcacg ctcgtcgcca
     4321 ctctctccgt tttgagcgca ctcgtcgcgc ttctctggtg gcggcgaatc gtgacttccg
     4381 ccgtgacgga tttagacgcg gcgacgggcg tgccgcgcgt gtgggtcgaa ggcttctggt
     4441 atgcgggctt gcccgtctcc gaatatctcg cgtggaaggc ggccttcacg ttcttaacca
     4501 tcctgctcat ctggcgcggc tggaagattg atgcggcgcg cacgcgcggc ttcgtgctcg
     4561 cgtgtgcgct cgcgctcgtc tgtttcgtcg agccgttttt cgtcgtcgcg cagtggtggc
     4621 aaccgtttgc gaagtcgcac gcgcgcttca acacggctgc gcccgtcacg cgtctgttgc
     4681 aggcgcggga ggggcaggcg ggacgggcga cgctcccgcg aagtcgcatc tacacgcgcg
     4741 tcaatctgtt cgcggaggag tttacggaat cgccgcgttt tgatccgccg aacttaaccg
     4801 cgctgtacgg cctggagaac gtcggcggct atgaacctct gttgctcgaa cgctacagcc
     4861 gcgcgctcgg caacgtcaat ctcgatgccg ccagcccgct ccccggctcc gcgccgaacc
     4921 gcgccctctt cgacgcacgc tcgcacgtcc tcgatctgct cggcgcaaaa ttcgtcgtca
     4981 acgtgaacat gtcgaaggct gacgcgccgc cggttgagac tgatctggac gcgcaacgct
     5041 gggctgtgat ccatgatgcc gacaacgtgc gcgtgcttga gaaccgccgc gcactgccgc
     5101 gcgcatggct cgtcggcgaa gccgcgtcgg ttgacgggga agaagccctg cggcgcatca
     5161 gcggcgcggg cgacagggag ttcgatccgc gacgcactgc gctcctcgaa gttgcgcccc
     5221 cggagatgcc cgcgctggca ggcggcgaac tcgcaccgga gagcaccgaa gcgcgcatca
     5281 cgctctatga accgaaccgc ctcgtcgtcg agacgcgtgc cgcgcaaccg tccgcactcg
     5341 tcgtcgcgga aattttttac cccggttggg aagcgacgat tgacggcacg cccgcgcgca
     5401 ttcatctcac gaatttcatc atgcgcggcg tggccgtgcc tgcgggcgcg catcgagtgg
     5461 agatgcgcta caccgcgccc gccgcacgca acggcgcgat catctccgcg ctcacgctcc
     5521 tcctgttgat tgttctggcg attcgtgggc gcacgcgcgg cggcgcggct tagagcgcgt
     5581 ctgaaagtta cctgctcgca ctttgctctg cgtctctagc gcgagcttgt tccttgagca
     5641 agcgcaagtc tcgcgcaggg gaacgaaggc gcagagaaga gacgacgcgg attttgaaac
     5701 gcgcgcttat agtttgaacg tgaagattcc cgaaacgagt aaaggcgtat ggcggcgaag
     5761 atgtgcggcg cggcgcggcg acgcggcggc gatgctctgt atcgtcgcct tcttctgcct
     5821 gttcttcggg tgggcgatct ttcgcggcca ttttctcatc ggcggcgacg tgttcttcta
     5881 cacttatcct ctgcgctccg tcgcttggga gatgatcagg caggggcaac tgccgctctg
     5941 gacgccgcat gtgctctccg gctacccgct cctttcgatg gcgcaactcg gcctcggcta
     6001 cccgctcacg tgggcgcacc tgttcctgcc cgcgcgccat gccgagttga tctacgtcct
     6061 cgcgccgttc ctgctcgcgc ccgcgttcac ctacgcctac ctgcgcgaac tcggacgcag
     6121 ccgcaccgcc gcgctcctcg ccggactctc tttcgcctac ggcggcctga tgaccaacac
     6181 ctacggcatg aacgccatcc cgacaaacgc cttgatgtgg ctgccgctcc tgctcatcgc
     6241 catcgagcgt gtgcgaacac gcggcctcgc ccgttccgtc gccgccgcgg cgcttccctg
     6301 cgccatgtcc gtcttaaccg gacacgggca gagcttcctc ttcgtctgcc tgctggcgac
     6361 cgcttacgct ctctttctcg gcttcgctta cgacgcgggc gacgcgcaag agaaccggcg
     6421 ggagcatgaa ccggagagcg aggcggggca cggacgcgcg cggaggcgtg cgtggttcgg
     6481 gtggcaacgc tggtcgccgc tgctggcttc gacgggcgga atgttactgg ccgcaggcat
     6541 cgcggcgttt caaattctgg agacgatgcg cgccgcgcgt cgcagcatcc gcagcaactt
     6601 gagttacgag tttttcgtcg aaggctcgtt tacgccgctc gccgcgtggc gttccttcgt
     6661 cgcgccgctc tatcacttca tcgaggtgac gacttacatc tcgccgctcg ctttcgcctg
     6721 cgccgtctgc gccgtcgcgt tcaatttacg tcgaggccga agacagacgc cgcaccgcgc
     6781 cgccttgacg atagacgtgc aggcgggaac ggacgcacgc gtcttcttct gggcagcgac
     6841 cgcgctcgtc gcagtgctcc tgatgctcgg cgggaacacg ccgctctatc gcgcgctcca
     6901 tcaagtgcct gtcttcaacc tgtttcgcag gccgtcgcgc cacgtctttg aatggacgtt
     6961 cgccctgagc gtgctcgcgg cctatggttg ggatgcgctc gattcattct cgcggcgcgg
     7021 cgtgagcggc gcgcgcgggg gcgtcgagcg cgggaaactc gtcgtcgcca cgctttcact
     7081 tttaatcgcc gccgctctcg gcgtcgtctg gtggcagacc gcgcatggcc tgccttatgt
     7141 gggcgacgcc gtgccgcgcg ctgagtccat ctatctcaag tggaagattc tcttcacgct
     7201 cgcgctcgcc gtcgcgtgcg gtttcgcttt gcgcctgagc gcggcgcggc gtcggcgtgc
     7261 gtggctgctc gcgtgcgtcg tgctgctcgc atgtttcgtc gagtcgcacc tgctcgtcac
     7321 gcactggtgg ccgggcacga tcaagactac ggagcggctc accactccgg cgcgtgcgac
     7381 gcgttacctg caacagtttc cggcgcacga gaaccgcgtc tacgtgcggg cgaattcggg
     7441 ggaagaagag tctgccgccg cgccgcgctt cgacgcgctc aacctgacgg cgcgctacgg
     7501 gctgcacaac gtcgcgggtt atgaagcgtt gttgtttgaa cgctacagcc gcgcgctcgg
     7561 agatgtggac tacgacgcgg tcaacccgcg acgcggcgca agggcgacgc tcgcgctctt
     7621 tgcgccccgt tcgcacgtgc tggacttatt gaacaccacg cgcgtcgtca cgtttgcgaa
     7681 cctcgcaaca acgcccacgg ttgaagcgcg gggcgcagag gcggacggcg atgcgctcga
     7741 cgccacgcgc tggcagaggg aagaagagtt tgacggggtc ttggtcctgc gcaacgaacg
     7801 cgccctgccg cgagcgtggc tggtgggaga agccgaagcg ttggacggcg aagaagccct
     7861 gcgccggatt cgcggcgaaa gcctcaccgc gttcgacccg cgccgcacgg ctctgctgga
     7921 agtgaaaccg gaagagttg
//
LOCUS       DASWNS010000303         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_220844, whole genome shotgun sequence.
ACCESSION   DASWNS010000303 DASWNS010000000
VERSION     DASWNS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220844"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1140)
                     /gene="nadA"
                     /locus_tag="VGB76_10775"
     CDS             complement(31..1140)
                     /gene="nadA"
                     /locus_tag="VGB76_10775"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY0099421.1"
                     /translation="MTVATLTKAPVLEDYFGLSDAELHERIERARRTLGERLVILGHH
                     YQRDDVICHADVTGDSFKLSKLGAGRDAAEFIVFCGVHFMAESADILSAPHQKVILPD
                     LGAGCSMADMASAEQVEDAWDQLASIGILSDFRVMPITYMNSTAAIKAFCGRNEGVVC
                     TSSNAVPLFEKSLREADKLFFFPDQHLGRNTGVKFGIPLEQMVVWDPFQELGGNTVEE
                     LRGARVILWKGHCSVHGRFREWHVDQVRKETPGIKVLVHPECVREVVEKSDLNGSTEF
                     IIKTLEDAPAGSSWAIGTEVNLVNRLIERFPDKHIRLLAPDLCMCATMYRIAPQNLAW
                     TLESLLAGVVPNQIIVPEETARWARVALDRMLAIK"
     gene            complement(1436..2881)
                     /gene="fumC"
                     /locus_tag="VGB76_10780"
     CDS             complement(1436..2881)
                     /gene="fumC"
                     /locus_tag="VGB76_10780"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY0099422.1"
                     /translation="MSQQEAKELREDSGAAQQEQATGTRTERDSMGDIEVPADRYYGA
                     QTQRSLIHFNIGFDTMPREMIRALGLLKKAAAFVNEELGKLPADKARLISAACDEVIA
                     GTLDEHFPLRVWQTGSGTQTNMNANEVISNRAIEMAGGRMGSKEPIHPNDHVNMSQSS
                     NDTFPTAMHIAAAEQMNRLIPEVERLHAAIDAKAKEYADVVKIGRTHLQDATPLTVGQ
                     EMSGWASLVERDIERLRTTLPGLYDLAIGGTAVGTGLNAHPEFAERAAQKIADLTGLP
                     FKSHPNKFAALSAHDELVFASGALKTLAGSLMKIANDIRWLASGPRAGFGELILPENE
                     PGSSIMPGKVNPTQSEAMTMVAVQVFGNDAAIGFAGSQGNFELNVFNPVMIHNLLHSI
                     RLIKDASHGFVDYCVAGIELNREQIKAHLNNSLMLVTALNPHIGYDRAAQVAKNAHKK
                     GISLRESAIALGYLTGEQFDEYVKPEEMTHP"
BASE COUNT          590 a          927 c          950 g          645 t
ORIGIN      
        1 ttactcatca ctcatcactc atcactatct ttacttgatt gcaagcattc tatcgagggc
       61 gacgcgcgcc cagcgggcag tctcttcagg cacgatgatc tggttcggca cgacgcctgc
      121 gagcagagat tccaacgtcc acgcgaggtt ctgcggcgcg atgcgataca tcgtcgcgca
      181 catgcagaga tcgggcgcga gcaggcgtat gtgcttgtcg ggaaaacgct cgatcaggcg
      241 gttgaccaag ttgacttccg tgccgatagc ccaggaggag ccggcgggcg catcttccag
      301 cgtcttgatg atgaactcgg tcgagccgtt caggtcggac ttctcgacca cctcgcgcac
      361 gcattcggga tgcacgagca ccttgatgcc gggcgtctcc ttgcgcacct gatccacgtg
      421 ccactcgcgg aaacgaccgt gcaccgagca gtggcctttc cagagaatga cgcgcgcgcc
      481 gcgcaactct tccacggtgt tgccgccgag ttcctgaaac gggtcccaga cgaccatctg
      541 ttcgagcggg atgccgaact tgacgcccgt gttgcgcccc agatgctggt ctgggaagaa
      601 gaacaattta tccgcctcgc gcaaactctt ctcgaagagc ggcacggcgt tcgacgaagt
      661 gcagaccacg ccttcgttac gtccgcagaa ggctttgatc gcagccgtcg aattcatgta
      721 ggtgatcggc atcacgcgga agtcacttag gatgccgatg gaagcgagtt gatcccaggc
      781 gtcttcgacc tgttcggctg aagccatgtc ggccatcgag cagcccgcgc cgaggtctgg
      841 caggatcact ttctggtgcg gcgcggagag aatgtccgcc gactcggcca tgaaatgcac
      901 gccgcaaaag actataaact ccgccgcgtc gcgccccgcg ccgagcttgg agagcttgaa
      961 ggagtcgccc gtcacgtcgg catgacagat cacgtcgtcg cgctggtagt gatgaccgag
     1021 gatgacgagc cgctcgccga gcgtgcgccg cgcgcgttcg atgcgctcgt gcaactccgc
     1081 atccgacaac ccgaagtagt cttccaggac aggcgctttc gttaaagttg ccacagtcat
     1141 tcttcgtccc tcctcaagcc ttctcagccg ccaatgacgg cctcacggcg cacggcgcaa
     1201 gcgaaaacta tctcactttg agaaacattg cgcaagcacc tgctttcgcg cccacgcgcc
     1261 tcataaaaga gtacgatacg gggcatgatt tagatgcaaa tgagatgcgt gcggatgtgc
     1321 ggatgaagcg agtaggagga gggggaaagg gaaagaaaag caggagcgcg cgaatcgttc
     1381 gcacgtctat gccgccatct ctcccttttc gcccttcccc ttttcccgaa tgttctcaag
     1441 ggtgcgtcat ctcttcgggc ttgacgtact cgtcgaactg ctcgcccgtc aggtagccga
     1501 gcgcgatggc agactcgcgc agggagatgc ctttcttgtg cgcattcttg gcaacctgcg
     1561 cggcacggtc gtagccgatg tgcgggttca gggcggtgac gagcatcagc gagttgttga
     1621 ggtgcgcctt gatctgctcg cggttgagtt cgatgcctgc gacgcaatag tctacgaagc
     1681 cgtgagaggc atctttgatg aggcggatcg agtggagcag gttgtggatc atcaccgggt
     1741 tgaagacgtt caactcgaag ttgccctgcg accccgcgaa gccgatggcg gcgtcgttgc
     1801 cgaagacctg cacggctacc atcgtcatcg cctcgctctg cgtcggattg accttgccgg
     1861 gcatgatgga ggagccgggt tcgttctccg gcaggatgag ttcgccgaac cctgcgcgtg
     1921 gtccggaggc gagccagcgg atgtcgttcg cgatcttcat gagcgacccg gcgagcgtct
     1981 tgagcgcgcc gctggcgaag accaactcgt cgtgcgcgga gagagccgca aacttgttcg
     2041 ggtgcgactt gaagggcagc cccgttaagt cggcaatctt ctgcgcggcg cgctcggcaa
     2101 actccgggtg cgcattgagt cccgtgccga ccgccgtgcc gccgatggcg agatcgtaaa
     2161 gacccggaag cgtcgtgcgc agccgctcga tgtcgcgctc gacgaggctc gcccagccgg
     2221 acatctcctg cccgaccgtc aggggcgtcg cgtcctgcaa gtgcgtgcgc ccgatcttca
     2281 ccacgtctgc gtactctttc gccttcgcgt caatcgccgc gtgcagacgc tcgacttcgg
     2341 gaatgagccg gttcatctgc tctgcggcgg cgatgtgcat ggccgtaggg aacgtgtcgt
     2401 ttgaagactg cgacatgttg acgtgatcgt tcgggtggat tggctctttg ctgcccatgc
     2461 gcccgcccgc catttcgatg gcgcggttcg agatcacttc gttggcgttc atgttggtct
     2521 gcgtgccgct gcccgtctgc cagacgcgca gggggaaatg ctcgtcgagc gtgcccgcga
     2581 tcacttcatc gcacgccgcg ctgatgagac gcgccttgtc cgcaggcaac ttgccgagtt
     2641 cttcattgac gaacgcggcg gccttcttta acagaccgag cgcgcggatc atctcgcgcg
     2701 gcatcgtgtc gaagccgatg ttgaaatgaa tcagagagcg ttgcgtctgc gcgccgtaat
     2761 acctatcggc gggcacttcg atgtcgccca tcgaatcccg ctcggtgcgc gtccccgtcg
     2821 cctgctcctg ctgcgccgcg ccgctgtcct ctctcaactc cttcgcttcc tgctgtgaca
     2881 taaacgcatc cgtccttcaa ttgactcaag cagacttccc atcaagttat acgtctcgcg
     2941 cgggacagtc tcactctaaa atttatgtga aaacgatgtg tgattgtaca acggggaaag
     3001 gttaaggcgg aaaggggaag ggatgaagca gcgcacgcaa acgatgggga aagtgatgag
     3061 tgatgagtga tgagtgatga gttgaagcac tcaatctctt gaaggcttta ac
//
LOCUS       DASWNS010000304         2756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_63142, whole genome shotgun sequence.
ACCESSION   DASWNS010000304 DASWNS010000000
VERSION     DASWNS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63142"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /gene="kdsA"
                     /locus_tag="VGB76_10785"
     CDS             complement(<1..767)
                     /gene="kdsA"
                     /locus_tag="VGB76_10785"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY0099423.1"
                     /translation="MATETQVQSFQVGNATFGDGRLTIIAGPCVIESPEHALMLAREC
                     AARTRAAGLDYVFKASFDKANRSSHESFRGRGMEEGLEVLQRVKEEVGVPVLTDIHDV
                     SQVEAVARVADVLQIPAFLSRQTDLVVAAARSGRVVNVKKGQFLAPADARNIVSKARA
                     AGCERLLLTERGVSFGYNNLVVDMRSFPIMRSFGAPVVFDVTHSLQLPGGLGHATGGQ
                     SEYIEPLARAGVACGVDAVFMEVHEEPSRSLSDGPNAL"
     gene            complement(864..2462)
                     /locus_tag="VGB76_10790"
     CDS             complement(864..2462)
                     /locus_tag="VGB76_10790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0099424.1"
                     /translation="MLHCPTCGRLFTADVKACPDDGATLRADATIAVPSPHDPLAGRV
                     LDEKYRLDERLGEGGMGTVYRATHLLIDRPVAVKVLHPRFVEDEAAQERFRREARAAG
                     RLQHTNAVTVTDFGRTADGFVYIVMELLEGRNLREVLAFESPLESRRAVALMLQVAAA
                     VEAAHESGVIHRDLKPANIFIVQPKNAPPLVKVLDFGIAKLAADTFDDSEQHALTQTG
                     VMIGTPRYMSPEQCDGAHLTPASDVYSLGIILYEMLTGKTPFTGPSPLAVALQHSSKP
                     PQPPTGLVANIPLELERVVLHALEKRPSNRPPDAGAFRLELQAAATRAGVAPPSHGYL
                     APGEFENGDRAQQPTGRYVLDNRETGGAARAGVRTNETTMLTDTSEKRRLQTGSVAAA
                     AATGALAINAAPTLASHPFTRVRVLLHDRRTLLYWLKQPPVLLATGLALLVLAVGLTA
                     FVRARNSGATIPGESANVAAAPTPSPEATPTPAPSPEEAANKRAQAGKSRRASRPATN
                     RNNNRKGSKVGGAFKKLKKILNPF"
BASE COUNT          523 a          851 c          869 g          513 t
ORIGIN      
        1 agcgcgttcg ggccgtcgga gagagagcgc gaaggctcct cgtgaacctc catgaagacc
       61 gcgtccacgc cgcaggccac cccggcccgc gcgagcggtt cgatgtactc cgactgcccg
      121 cccgttgcat ggccgaggcc gcccggcagt tgcaggctat gcgtcacgtc gaagacgacg
      181 ggcgcgccga aactccgcat gatgggaaac gagcgcatgt caacgacgag attgttgtag
      241 ccgaaggaga cgccgcgctc cgtgagcaac aggcgttcgc acccggcggc acgcgcttta
      301 ctgacgatgt tgcgcgcatc ggcgggcgcg agaaattgtc ccttcttcac attcacgacg
      361 cgcccggaac gcgccgccgc gacgacgagg tcggtctgcc tggagaggaa cgccggaatc
      421 tgcaacacgt ccgcgacgcg cgccaccgct tcgacttgag atacgtcgtg aatgtccgtc
      481 aacacgggca cgccgacctc ctctttcacg cgctgcaaaa cctccaagcc ttcctccatc
      541 ccgcgaccgc gaaaactttc gtgcgacgag cggttggcct tgtcgaaaga ggctttaaag
      601 acatagtcga gtccggcggc gcgcgtacgc gcggcacatt cacgcgcgag catcagggca
      661 tgctccggcg attcgattac gcagggaccc gcgatgatcg ttaaacgccc gtcgccgaag
      721 gtcgcgttgc cgacctgaaa agattgcacc tgtgtttcgg ttgccataga tgattcgtta
      781 gcagctctta aaaaaagttt gagcagtcgc gccagtgacg aagaaaaacg tgacgaggaa
      841 aaatcctgcg ttcccgtcag tctctaaaac ggattcaaaa tcttctttaa ctttttgaaa
      901 gccccgccga ccttcgagcc tttccggtta ttgtttcgat tcgtggccgg gcggctcgcg
      961 cgccgggatt ttccggcctg cgcccgcttg ttcgccgctt cttcaggcga gggcgcggga
     1021 gtcggcgtcg cttccggtga aggcgtgggc gcggctgcga cgttcgcgct ctcgccggga
     1081 atcgtcgcgc cgctgttgcg cgccctgaca aacgccgtaa ggccgacggc cagcacgagc
     1141 aaggccaagc ccgtcgccag caacacgggc ggctgcttga gccagtagag caaggtgcgg
     1201 cggtcatgca acagaaccct gacgcgcgtg aacgggtgcg aggcgagcgt cggcgcggcg
     1261 ttgatggcga gcgcgccggt tgccgcagcg gcggccacgc tgcccgtttg cagcctgcgc
     1321 ttctccgacg tgtccgtgag catcgtcgtc tcgttcgtac gcacgcccgc gcgcgctgct
     1381 ccgcccgtct ccctgttatc gaggacgtat cgcccggtcg gttgctgcgc cctgtcgccg
     1441 ttctcaaact ctcccggcgc gagatagccg tgagacggcg gggcgactcc tgcgcgcgtg
     1501 gccgccgctt gcagttcgag tcgaaacgca ccggcatcgg gcgggcggtt cgaaggtctt
     1561 ttttcgagcg cgtggagcac gacgcgttcg agttcgagcg ggatgtttgc gaccaacccg
     1621 gtcggcggtt gcggcggctt cgaagaatgt tgcagcgcga cggcgagcgg cgaggggcct
     1681 gtgaagggag tcttgccggt cagcatctcg tagagaatga tgccgaggct atacacgtcc
     1741 gaggcgggcg tgagatgcgc gccgtcgcat tgttcgggcg acatgtagcg gggcgttccg
     1801 atcatcacgc ccgtctgcgt cagcgcgtgc tgctccgagt cgtcaaaggt gtcggcggct
     1861 agtttggcga tgccgaagtc caggactttg acgaggggcg gcgcgttttt cggttggacg
     1921 atgaagatgt tggcgggttt cagatcacga tgaataacgc cgctttcgtg cgccgcttcg
     1981 accgccgccg ccacctgcaa catcagcgcg accgcgcgcc tgctctcaag cggcgactcg
     2041 aaggccagca cctcgcgcag gtttcgccct tcgagcaact ccatcacgat gtagacgaag
     2101 ccgtccgccg tgcgcccgaa gtccgtcacc gtcacggcgt tcgtgtgttg gagtcgcccg
     2161 gcggcgcgcg cctcgcgccg gaagcgttcc tgcgccgcct cgtcttcgac gaaacgcggg
     2221 tgcaaaactt tcaccgccac ggggcgatca atcaagaggt gcgtggcgcg atagaccgtg
     2281 cccatcccgc cctcgccgag cctctcgtcc agacgatact tttcatccaa cacgcgcccc
     2341 gcgagcgggt cgtgcggcga cggcacggcg atggtcgcgt cggcacgcaa agtcgccccg
     2401 tcatccgggc acgccttcac gtccgccgta aacaaccgac cgcatgtagg acaatgaagc
     2461 ataatttcct aaagacagtg acaagtgacg agtgacaagt gacgggttaa aacagtaaat
     2521 cgtgaatcgt caatcgtaaa tagacgaaga agaatttcct ctattcacga gtcacgattg
     2581 acattcaagg cttcatctcg tcactcgtca ctcgtcactt atcactgctt tattgtcggt
     2641 ttttcgaagc aaaaagtaca gcaggaagaa agggttaagg gcgggcggat cagggatgaa
     2701 ggatcaggga tgagggatga atcaaagccc cgaatcgcga atcgtcaatc gtgaat
//
LOCUS       DASWNS010000305         5957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_427696, whole genome shotgun sequence.
ACCESSION   DASWNS010000305 DASWNS010000000
VERSION     DASWNS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427696"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="trpD"
                     /locus_tag="VGB76_10795"
     CDS             <1..571
                     /gene="trpD"
                     /locus_tag="VGB76_10795"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY0099425.1"
                     /translation="FMFAPLYHGATARVAGVRRELGVHTTFNLLGPLTNPAGAPRQII
                     GVWHASLVEPLARTLAALGTERAWVVHGRDGLDEITISDKTFVAEASGGLVHTFHIAP
                     EDFGIERARLDELRGGDPEMNARIIRSVLGGERRDAARSLVVANAAGALYVGGAAATL
                     HEAAELAARSIDSGAALDKLRRLAEATNR"
     gene            568..1377
                     /gene="trpC"
                     /locus_tag="VGB76_10800"
     CDS             568..1377
                     /gene="trpC"
                     /locus_tag="VGB76_10800"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY0099426.1"
                     /translation="MNAPPDFLRRIIEQKRVRLSRAMNSRPLEELRAAASDVRSEAKP
                     HAFRAALERAGRFHVIAEIKRASPSLGDIRTDISPAALAREYESGGASAISILTEEDH
                     FKGSLPDLREVRAHTSLPLLRKDFIFEEWQVYEAAAARADALLLIVAALEDATLARLL
                     SITEAELGMDALVEVHTAEELRRAAVCGAKVIGVNNRNLHTFEVSLETSVELATHAPQ
                     NALLVSESGLRTQADLRRLASLGYRGFLVGETLMRAAHPAEALRALLAETS"
     gene            1471..1782
                     /locus_tag="VGB76_10805"
     CDS             1471..1782
                     /locus_tag="VGB76_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099427.1"
                     /translation="MNYVDDGHVVDDLRQLARQANGERITIQWIPDSPPQTRLPQRVL
                     KSIANYKAHLPAHVRNSGADIAVIREFRTDIFLKPGKQFAVESYLLDDRGREHVCRVS
                     F"
     gene            1808..2464
                     /locus_tag="VGB76_10810"
     CDS             1808..2464
                     /locus_tag="VGB76_10810"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY0099428.1"
                     /translation="MVKVKVCGITNLEDALAAVEAGADALGFNFYRRSPRYLAPDAAR
                     RIISELQQFGGVLCVGVFVNEDSAATVVRMAAESGVAAVQLHGDETPEYCAELSGQRV
                     IKALRVGKDFAPEQATAYRAESVLLDAYSASARGGTGETFDWELARRTREVVPQLYLA
                     GGLTVENVAEACATVEPYAVDVCSGVELAPGRKEAARVRAFVSAVRAASKKLDLQPSR
                     "
     gene            2593..3540
                     /locus_tag="VGB76_10815"
     CDS             2593..3540
                     /locus_tag="VGB76_10815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0099429.1"
                     /translation="MSATARATSNQANSREQGRVLVVTDETSHAASFDALEEAGLVVV
                     GVASGAAAIVALRKSRPHVVLLDTSLRGISAEELTRHVVREQDGVPLVLFGDAEATHE
                     RRHATLSAGAFDYFQLPCELALLIARTEQLVKLRQAMDRLRAEADRDYLTGLANRRRF
                     RTALGQELERWRRYKVACALLLVDIDFLKRINDAHGHSAGDECIRHVASALTELSRDN
                     DTAARLGGEEFALLLAGATEASAFAAAERLREAVSSHEIENIGFVTVSIGVAACPAHA
                     TSERTLYAAGDAALYRAKGEGRNRSTLAASLSPANASVL"
     gene            3730..4998
                     /gene="trpB"
                     /locus_tag="VGB76_10820"
     CDS             3730..4998
                     /gene="trpB"
                     /locus_tag="VGB76_10820"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY0099430.1"
                     /translation="MPENFLTQPDEHGHWGAYGGRYVPETLMAPLEELNAAYAAVRVD
                     ETFQAEFAALLKDFSGRPTPLFYAARLTAHAGGARIFLKREDLSHTGSHKINNALGQA
                     LLARRMGKRRIIAETGAGQHGVASATVCALFGLQCVVYMGAEDVRRQRLNVFRMELLG
                     AEVREVQAGARTLKDAINEALRDWVTNVADTYYLLGSALGPHPYPLMVRDFQSVIGRE
                     ARVQFVEREGGRLPDALVACVGGGSNSIGLFHPFLGDAGVRMIGVEAGGSGDKLGEHA
                     ARFNVSGGGRPGVLQGTMSYVLQDERGQIAATHSISAGLDYPSIGPEHAFLHDAGRVE
                     YVSATDADALAAFRLLSEQEGIIPALESAHAVAYALKLASEMKPEQSILVNLSGRGDK
                     DVQSVADDAARPENPTERAHGETLGGVELP"
     gene            4995..5783
                     /gene="trpA"
                     /locus_tag="VGB76_10825"
     CDS             4995..5783
                     /gene="trpA"
                     /locus_tag="VGB76_10825"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY0099431.1"
                     /translation="MSRIAETFARLRREQRRGFIPFITAGDPDLETTRRLIPELARAG
                     ASVIELGVPFSDPMADGPVIQRASERALRGEVGVAEVLSVVREARRETDVPIVLFSYY
                     NPLLQYGAERLCAEASRAGVDGMLVTDLAPEEAGEFAATLRAHELDLIFLVAPTSTDA
                     RLKRIAERASGFIYAVSRAGVTGVQNALSTEAGRLVARVRAVSELPVAVGFGISQREQ
                     VAEVWRFADAAVVGSAIVAEIEKRGDSPDLVAHIGAFARALLPS"
BASE COUNT         1068 a         1831 c         1985 g         1073 t
ORIGIN      
        1 cttcatgttt gcgccgctct atcacggcgc gacggcgcgc gtcgcgggcg tgcgccgcga
       61 actcggcgtg catacgactt tcaatctgct tggtcctttg acgaaccctg cgggcgcgcc
      121 gcgacagatc atcggcgtct ggcacgcatc gctcgtcgaa ccgctggcgc ggacgctcgc
      181 cgcgctcggc acggagcgcg cgtgggtggt gcacgggcgc gacggtctgg atgagatcac
      241 gatcagcgat aagaccttcg tggccgaagc gtcgggcggc ctcgtgcata cttttcatat
      301 cgcgccggaa gatttcggga tcgagcgcgc gcgcctcgat gagttgcgcg gaggcgaccc
      361 ggagatgaac gcgcgtatta ttcggagcgt gctcggcggt gaacgacggg acgcggcgcg
      421 ttcgctcgtc gtcgccaacg cggcgggcgc gctctacgtc ggcggcgcag cggcaactct
      481 gcacgaggcc gccgaactcg ccgcgcgcag catcgacagc ggagccgcgc tcgacaaact
      541 gcgacgactc gcggaagcca ccaaccgatg aacgcgccgc ccgattttct cagacgaatc
      601 atcgaacaga agcgcgtgcg cctgtcgcgt gcgatgaact cccgcccgct cgaagagttg
      661 cgcgcggcgg cttccgacgt gcgaagcgaa gcgaagccgc acgcgtttcg cgccgcgctc
      721 gaacgcgccg gacgcttcca tgtcatcgca gagatcaagc gtgcctcgcc ttcgctcgga
      781 gacatccgga cggacatctc gcccgccgca ctcgcacgcg aatacgaatc gggcggggcg
      841 tcggccatct ccatcctgac ggaagaggat catttcaaag ggtcgctgcc agatttgcgg
      901 gaggtgcgcg cgcacacgtc tttgccgctg ctgcgaaagg atttcatctt cgaagagtgg
      961 caggtatacg aagccgccgc cgcccgcgcc gatgcgttgc tactgatcgt cgccgcgctc
     1021 gaagatgcga cgctcgcgcg cctcctgagc atcaccgaag cggaactcgg aatggacgcg
     1081 ctcgtcgaag tgcacacggc ggaagagttg cggcgtgccg ccgtgtgcgg cgcgaaagtc
     1141 atcggcgtca acaatcgcaa cctgcacacc tttgaagtgt cgctcgaaac ttccgtcgaa
     1201 ctcgcgacgc acgcgccgca gaacgcgctg cttgtcagcg aaagcggcct gcgcacacag
     1261 gccgacctgc gccgcctcgc atcgctcggc tatcgtggct ttctcgtcgg cgaaaccttg
     1321 atgcgcgcgg cgcacccggc ggaagcattg cgcgcgctgt tagcggagac gagttgaggc
     1381 gagcgggcgc aagttatacc gttgtgaatt atgaaataca gtgtgctcaa atcggtcgcg
     1441 cataacttct ctcactcgtt cgtgagtggg atgaattatg tggatgacgg tcacgtcgta
     1501 gacgatctac gtcaactcgc gcgacaggct aatggcgagc gcatcaccat ccagtggatt
     1561 ccggactctc cgccgcagac gcgtctgccg cagagagttc ttaaaagtat cgctaactac
     1621 aaagctcatc tcccggcgca tgtgcggaac tcgggcgcag atattgcggt catacgcgag
     1681 ttcagaaccg acatctttct gaagcccggc aagcagtttg ccgtggagtc gtacctgctg
     1741 gatgatagag gccgggagca cgtttgcaga gtctcgtttt aggctcgcgc ggcgtaagag
     1801 aagacggatg gtgaaagtta aagtctgcgg catcacgaat ctggaagatg cgctcgcggc
     1861 ggtggaggcg ggcgcggacg cgctcggctt caacttttac aggcgcagcc cgcgttacct
     1921 cgcgccggat gcggcgcgga gaattatctc ggagttgcag cagttcggcg gggtgctgtg
     1981 cgtcggcgtg ttcgtcaatg aagatagcgc ggcgacggtg gtgcgcatgg ctgcggagtc
     2041 gggcgtggcg gcagttcaat tgcacggcga cgagacgccg gagtattgcg cggaattgag
     2101 cgggcagcgc gtcattaaag cgttgcgcgt ggggaaagat tttgcgccgg agcaggcgac
     2161 ggcctaccgt gcggagtcgg ttctgctcga cgcttatagc gcgagcgcac gcggcggcac
     2221 gggcgagacg ttcgattggg aactggcgcg caggacgcgc gaagtggtgc cgcagttgta
     2281 tctcgcgggc ggcctcacgg tggagaacgt ggcggaggcg tgtgcgacgg tcgagcctta
     2341 cgcggttgat gtgtgcagcg gcgtggagct tgcgcccgga cgaaaagagg ctgcgcgggt
     2401 gcgcgctttt gtctcggcgg tgcgcgcagc gtcgaaaaag cttgacttgc agccgtcaag
     2461 atagagtatg ctgtcggcgt agcgaacgtg agatgcgcgc cgcccgctaa cgcggcacag
     2521 cgcgaccctt gactgcccct ctacctcctc cgagttgttc attagatagc ttgcctttca
     2581 acgaagaaac agatgagcgc aaccgcgcga gcgactagca atcaggcgaa ctcacgcgag
     2641 cagggacgcg tgctcgtcgt gacggacgaa acaagccacg ccgcttcgtt cgacgcgttg
     2701 gaggaggctg gcctcgtcgt cgtgggcgtc gcgagcggag cggcggccat cgtcgcgctg
     2761 agaaagtcgc gcccgcacgt cgtccttctc gatacgtcgt tgcgcggaat cagcgcggaa
     2821 gagttgacgc gccatgtcgt gcgcgagcag gacggcgtgc cgctcgttct cttcggcgac
     2881 gcggaggcca cacacgaacg caggcacgcc acgctcagcg cgggcgcgtt cgattatttc
     2941 caactgccct gcgaactcgc gctcctcatc gcgcgcaccg aacaactcgt caaactcaga
     3001 caggcgatgg atcgtctgcg cgcagaagcc gaccgcgatt atttaacggg actcgccaac
     3061 cgtcgccgtt ttcgcaccgc gctcggacag gaactcgaac gctggcgtcg ttacaaagtc
     3121 gcctgcgcgc tcctgctcgt ggacatagat ttcctcaagc gcatcaacga cgcgcacggc
     3181 cattcggcgg gcgacgagtg catccgccac gtagcgagcg cgctcacgga actctcgcgc
     3241 gacaacgaca cggcggcgcg tctgggcgga gaagagttcg cgttgctgct cgccggtgcg
     3301 acggaggcga gcgcgtttgc cgccgcagag cgtctgcgcg aagccgtctc gtcgcatgag
     3361 atcgaaaata tcggcttcgt tacagtgagc atcggcgtcg ccgcctgccc ggcacacgcc
     3421 acgtccgagc gcacgctcta tgccgccggc gacgccgccc tctatcgcgc caaaggcgaa
     3481 gggcgcaacc gttccaccct cgccgcctct ctcagcccgg caaacgcatc ggtgctataa
     3541 tcccgcgaca acatcatcgt tcaactttgc gcctccggtt cttacatcct tcgcgtctcc
     3601 tgcgagaact gtatttcacg ccggacgcgc aaagttaaga cgcgaggcgc acagcgtcga
     3661 gttgtaattc aaggattgat actgctgctt agcgggcggg gcgcgagctg tctccgtcca
     3721 tacgatctta tgccggaaaa ttttttaacg cagccggatg agcacggcca ctggggcgca
     3781 tacggcggtc gctacgtgcc ggagactttg atggctccgc tcgaagagtt aaacgcggcc
     3841 tacgccgccg tccgcgtcga tgagacgttt caggcggagt ttgcggcctt gctcaaggac
     3901 ttctccggtc gtcccacgcc cctcttttat gccgcgcggc tgaccgcgca cgcaggcggg
     3961 gcgcggattt ttctcaaacg cgaagacctc tcgcacacag gctcgcacaa gatcaacaac
     4021 gcgctcggtc aggcgttgct cgcgcggcgg atggggaagc ggcgcatcat cgcagagacg
     4081 ggcgcgggac agcacggcgt cgcgtctgcg accgtgtgcg cgctcttcgg attgcagtgc
     4141 gtcgtctata tgggcgcgga ggatgtgcgg cgtcagcgtc tgaacgtctt ccgcatggag
     4201 ttgctcggcg cggaggtgcg cgaggtgcaa gcgggcgcgc gcactttgaa ggatgcgatt
     4261 aacgaagccc tgcgcgattg ggtgacgaac gtggcggaca cttattacct gctcggcagc
     4321 gcgctcggac cccacccgta cccgctcatg gtgcgagatt ttcagagcgt catcgggcgc
     4381 gaggcgcgcg tgcagttcgt ggagcgcgag ggcgggcgtc tgcccgacgc gctcgtggcc
     4441 tgcgtcggcg gcgggtcgaa ctctatcggc ctgttccacc cgtttctggg cgatgcaggc
     4501 gtgcggatga tcggcgtcga agcgggcggg agcggcgata aactcggcga gcacgccgcg
     4561 cgtttcaacg tcagcggcgg cgggcgaccc ggcgtgttgc agggcactat gagttacgtc
     4621 ttgcaggacg agcgcgggca gatcgccgcg actcattcga tctccgctgg cctcgattat
     4681 ccttcaatcg gaccagagca cgctttcctg catgatgcgg ggcgcgtgga atacgtctcc
     4741 gcaacagacg cggacgcgct cgcggccttt cgtctcttgt ccgagcagga aggaattatt
     4801 cctgcgttgg agtcggcgca cgccgtcgcc tacgctttaa agctggcgag cgagatgaag
     4861 ccggagcagt caattctcgt caacctctcc gggcgcggcg acaaggacgt gcagagcgta
     4921 gccgatgacg cggctcgacc ggagaacccg accgaacgcg cgcatggtga aacgttggga
     4981 ggcgtggaac tgccttgagt cgcatcgcag agactttcgc gcggctgaga cgagagcaga
     5041 ggcgcggctt catccccttc atcacggcgg gcgatcctga tctggaaact acacgccggt
     5101 tgatcccgga actcgcgcgc gcgggcgcaa gcgtcatcga actgggcgtg ccctttagtg
     5161 atccgatggc tgatgggcct gtcatccagc gtgcatcgga acgcgcgctc agaggcgaag
     5221 taggcgtcgc agaggtgttg tcggtcgtca gagaggcgcg gcgtgagacg gacgtgccga
     5281 tagtgctgtt cagttattac aaccctctgc tgcaatacgg cgcggagcgt ctgtgcgcgg
     5341 aagcctcgcg cgcaggggtg gacggaatgc tcgtcacaga cctcgcgccg gaagaggccg
     5401 gagagtttgc cgcgacgctc cgcgcacacg aactcgatct catcttcctg gtcgcgccca
     5461 cctcgacgga cgcacgcttg aaacgcatcg ccgaacgcgc aagcggtttc atttatgcgg
     5521 tttcgcgcgc gggcgtgacc ggcgtgcaga acgctctgag cacggaggcc gggcgtctgg
     5581 tggcgcgcgt gcgcgccgtc tccgaactgc ccgtcgccgt cggcttcggc atctcgcaga
     5641 gggaacaggt cgcggaagtc tggcgtttcg cggacgccgc cgtggtcggc tcggccatcg
     5701 tcgcggagat cgagaaacgg ggcgactccc ctgatctcgt cgcccacatc ggagccttcg
     5761 cgcgcgccct gctgccgtct taaagggcac cggcgcaaac atcgaaattg cgctcgactt
     5821 gggcgcggtc gctggcgtat cctaacgccg cgccggagac acctgcccgc cgtttgcgcc
     5881 tgcgaaattc tgacggcggc aagtttttgc gtctcacctg cgggaaaata aatttgttgc
     5941 atacatgaaa cagcgtt
//
LOCUS       DASWNS010000306         5217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_400927, whole genome shotgun sequence.
ACCESSION   DASWNS010000306 DASWNS010000000
VERSION     DASWNS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400927"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..1166)
                     /locus_tag="VGB76_10830"
     CDS             complement(411..1166)
                     /locus_tag="VGB76_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099432.1"
                     /translation="MSRKINSALAVLAMLAFANSSLATSYNTVDVSTGVNVGVVPNAL
                     YATTPGTKDGYWMVRSTPAGALPPNTGAWIVTFSGSWNVAPGTRPIFGNNNNVGTSEY
                     ERCFCLQSTEKSLLTLTMRADNKANLFLNSYFNPVMQAVANNTFNPAVPAVQFTYTAQ
                     NGLKVGRNCIRVRVNNEGGPTGFALKASLSGFGALDNADQPCCRQGAAVFSEALRGLP
                     AELDPTPSRENLDPRPDGERPRVRDVPAERPRP"
     gene            1615..1884
                     /locus_tag="VGB76_10835"
                     /pseudo
     CDS             1615..1884
                     /locus_tag="VGB76_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
     gene            2162..4105
                     /locus_tag="VGB76_10840"
     CDS             2162..4105
                     /locus_tag="VGB76_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0099433.1"
                     /translation="MSVDKDLVSVQQARDAVEAAHRAQIQLARFDQAQIDRICEAMAK
                     AALREATRLGAMAVEETGFGIPDDKREKNRFAAEDVWNAFRGLRTVGVVSESKDVIEI
                     ASPRGVVCGIIPSTNPTSTAIFKILISIKSRNAIVLSPHPSAAKCIAETARIMREVAI
                     KEGLPADAINCLTTATIEGTEALMKNRLTAVILATGGIGLVRAAYSSGKPAFGVGPGN
                     VPVFIERTADVPTAVQNILTGKTFDNGTICASEQAVVADAPVAKAVRAEFAQQGGHFL
                     SAAEAEQLAKVVTTAQRTLNPAIVGKSVEVIAKMAGLNVPQGTRCLMADVGGVGREHP
                     LSMEKLSPILAFYVEDGLERGAARCFEILSYGGMGHTAGIHTRSRDVAKAYGVEMPAS
                     RVVVNTPTTHGAIGFSTALPPSMTLGCGSWGGNVTSDNISPLHLMDIKRVAFETRPVK
                     SARPAQGAGQASAFAPQAAAPVAPQAGGKVSREDVAAIVDRFLSNRNASQPTPTFNAP
                     MRPNESPAPVQPMNEIDPNWVQSKSAAAPHAEAAPTAQASSGGGGNGGGNGGSSGGAN
                     ATVQAAASPASKPTAATVSGGNAQAQPTPTQTNGHKAVDFVSEEDVRRAIGKGEKIYV
                     NAKTIITPSARDIGDPAEVFAKV"
     gene            4199..4531
                     /locus_tag="VGB76_10845"
     CDS             4199..4531
                     /locus_tag="VGB76_10845"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEY0099434.1"
                     /translation="MFLGKVVGTVWSTKKTPDLEGVKFLIVHPIELDKEPNRSIIVVA
                     DRLGAGVGEIVMCAFGKAARSAIGDQEMSIEAAVVGIVDRVDVQGSPSDDLNETAHEL
                     RSGDNGRR"
     gene            4677..5162
                     /locus_tag="VGB76_10850"
     CDS             4677..5162
                     /locus_tag="VGB76_10850"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY0099435.1"
                     /translation="MANEELIYRITRAVYGRLGAQADERTVENLVTDIYRAIEPVVEA
                     PADGDNGATQRGARRGLTAHGGEAEGSSDRLIVSVFGVDHPGIVAGVAQILAESGCSI
                     ADINQTVVQGKFAMVIIADSSNSNAPVSTLKERFRAAGDKLGVRIYAQREDLFNAMHR
                     I"
BASE COUNT         1105 a         1497 c         1635 g          980 t
ORIGIN      
        1 ctggtcggag agttcgcgca gggaaaggcc gcgcgcctcg cgcgccgcgc gcaaacgcgc
       61 gccgagagac gactcaccgc cgccagctct cgcgtgttct tctgagttct ggtcaggagt
      121 cttagagttc acttggcgat tgtgcttcga ggagctaaac ggtcgcttgc gttaatgtct
      181 attagtctgt ttttgccagc cccgcgcggc aacgtcgcac gcgccgcaac tattggcgcg
      241 aatataactt gcgccgcgcg aacgtgtcaa actccggttt tttcaaaaga tacgaaggcg
      301 cgccccggct gctgaagatt tgcgacaaac cggaatccag agccgttgag gaactccgga
      361 ttccggtttg tctagctcga cgagttgcgt tttccgtcaa cggatatttt ttacgggcgt
      421 ggccgctctg ccggaacatc tcgcacgcgc ggtctttctc cgtccggcct cgggtctaaa
      481 ttctctctgg agggcgtggg gtctaactcg gcggggagtc ctctgagggc ttccgaaaaa
      541 acggctgcgc cttgcctgca acagggttgg tctgcgttgt cgagtgcgcc aaaaccgctc
      601 aaggaggctt tcagcgcaaa cccggtcggg ccgccctcgt tattgacgcg caccctgatg
      661 cagtttcttc cgactttcaa cccgttctgc gccgtgtaag tgaattggac ggcgggcacg
      721 gcaggattga aggtgttgtt ggctacggcc tgcatgaccg gattaaagta actgttgaga
      781 aacaaatttg ccttgttatc cgcgcgcatc gtgagcgtta agagcgactt ctcagtggat
      841 tgaaggcaaa aacatctttc gtattccgac gtgccgacat tattgttatt tccgaagatt
      901 ggtcgagtgc cgggagcgac gttccaactg cccgagaaag taactatcca tgctccggtg
      961 ttcggcggca gcgcgcctgc gggcgtcgag cgaaccatcc agtaaccgtc cttcgtgccc
     1021 ggcgtcgtcg cgtacagagc gttaggcaca actccgacgt tgacgcccgt gctcacgtca
     1081 acggtgttgt atgacgtagc aagactcgaa ttcgcaaaag ccagcattgc cagcacggct
     1141 aaagccgaat ttatctttcg tgacattttt tttctcctta gatttttctt ccacgtgttg
     1201 agagatgagg cgtggaagcc aatagcgggg tgaaggggcg tgcagcgcag ggaagcgtgt
     1261 gcagcgcggg gcgaggcggg tcggacgccg tctgagtgag caatagccat accagccgcg
     1321 cggccaatct caagcgatct ctttagcggc taaaaatgag ctttagtttc cggcgcgggc
     1381 gcgaggtgta gacagggaag gacgttcttc gccgggcggg tgtgcacttc tgttgagtcc
     1441 tgctttccgt tcaagaacga aaatggctgc cggaaggaaa gttcgcgtat tacgaaagag
     1501 gcgcgtccct tgattcttgc cgcgcacggt gctacgttga gccgttcaag gcaaccaact
     1561 ggaaacggaa acgtttttaa tttctacaat cacagaaaag cgaggagacg actcatgcct
     1621 ctcgaagcat tgggaatggt cgagacgaaa ggttttgtcg gcgcggtcga agcggctgac
     1681 gcgatggtca aggccgccaa cgtccaactt ttaggaaaag agtacatcgg cgcaggttac
     1741 gtgacgatct tcgtgcgcgg cgacgtgggt gcggtgaagg ccgcgacgga cgcgggcgca
     1801 gccgccgcac ggcgcgtcgg cgaattgatc agcgtgcacg tcattccgcg cccgcacggc
     1861 gaggtcgaac gcgtgttgcc gaagaacggg cgcacaaact acagcccgga cgaacgcgcc
     1921 gtcggcggtg gcggctcgcg cggcgaactc ggcgaaggcg accagggacg ctcactcccg
     1981 gcaggcgcac gcgaaaacaa cagggagaaa tcaaagtgac aagtggcgag aaagaaaagt
     2041 gatgagtgat gagtgatgag taatgagtaa aagacaagtc tttactcatc actcatcact
     2101 catcacccat tacttcttcc ttgtcactca tccctgtagt tgtcactgtc ttggaggctt
     2161 tatgtcagtt gacaaagacc ttgtctcggt gcagcaggcg cgcgacgcgg tcgaggccgc
     2221 acatcgcgca caaatccaac tggcgcgatt cgatcaggcg cagattgatc gcatctgcga
     2281 agcgatggcg aaggccgctc tgcgcgaggc gacacggctc ggcgcaatgg ccgtcgaaga
     2341 gacgggcttc ggcatccccg acgacaagcg cgagaagaat cgcttcgcgg cggaagacgt
     2401 gtggaatgcg tttcgcggcc tgcgcacggt cggcgtcgtc tccgaatcga aagacgtgat
     2461 agagatcgcc agcccgcgcg gagtggtgtg cggcatcatc ccttcgacga atccaacctc
     2521 gacggcgatc ttcaaaattc tcatctccat caagtcgcgc aatgccatcg tgctttcgcc
     2581 gcacccgtcc gccgcgaagt gcatcgcgga gacggcgcgc atcatgcgcg aagtggcgat
     2641 caaggaaggc ttgcccgccg acgcgatcaa ctgcctgacg acggccacca tcgaaggcac
     2701 ggaagccttg atgaagaacc gtctgaccgc cgtgattctg gcgacgggcg gcatcggcct
     2761 cgtgcgcgcg gcctattctt ccggcaagcc cgccttcggc gtcggccccg gcaacgtgcc
     2821 tgtcttcatc gagcgcacgg cggacgtgcc cacagccgta caaaatattt tgacgggcaa
     2881 gaccttcgac aacgggacga tctgcgcttc ggaacaggcg gtcgtcgccg acgctcccgt
     2941 cgcaaaagcc gtgcgcgcgg agtttgcaca acagggcggc cacttcctga gcgcggcgga
     3001 agcagagcaa ctggctaaag tcgtcaccac cgcgcagcgc acactgaatc cggcgattgt
     3061 cggcaagtcg gtcgaagtga tcgccaagat ggcgggcttg aatgtgccgc agggcacgcg
     3121 ctgcctgatg gctgacgtgg gcggagtcgg gcgcgaacat cctctgtcga tggagaaact
     3181 gtcgccgatt ctcgccttct acgtggaaga cggtctggag cggggcgcgg cgcgctgctt
     3241 cgagattttg agttatggcg gcatgggaca caccgccgga atccacacgc gttcgcgcga
     3301 cgtggcgaag gcttacggcg tggagatgcc cgcctcgcgc gtcgtcgtca acacgccgac
     3361 gacgcacggc gcaatcggtt tctcgaccgc cctcccgcct tcgatgactc tcggttgcgg
     3421 ctcttggggc ggcaacgtca cctccgacaa catctcgccc ctgcacttga tggacatcaa
     3481 gcgcgtcgcc ttcgagacgc gacccgtcaa gagcgcgcgc ccggcgcaag gggcagggca
     3541 ggcgagcgcg tttgcaccgc aagccgccgc tcccgtcgcg ccgcaggcgg gcggaaaagt
     3601 gagccgggaa gatgtcgcgg ccatcgttga ccgtttcctg tcgaaccgga acgccagcca
     3661 gccgacgccg accttcaacg cgcccatgcg cccgaacgaa tcgcccgcgc ccgtgcagcc
     3721 catgaacgag atcgatccga actgggtaca atccaagagc gcagccgccc cgcacgccga
     3781 agccgcgccc accgcgcagg cgagcagcgg cggaggcggc aatggcggcg gcaacggcgg
     3841 aagtagcggc ggcgcaaacg cgacggtgca ggcggcagcg tcgcccgcgt cgaagcctac
     3901 agccgcaacc gtctccggcg gcaacgcgca ggcgcagccg acgcccacgc agacgaacgg
     3961 gcacaaagcc gtggacttcg tctcggaaga ggacgtgcgc cgcgccatcg gtaaaggcga
     4021 aaagatttac gtcaacgcca agacgatcat cacgccctcg gcgcgcgaca tcggcgaccc
     4081 ggcggaagtg tttgcgaagg tctaaaaact ggtgaatcgt gaatcgtaaa tcgtgaatag
     4141 attaaatccg attctttcta ttcacgattt acgattcacg attcgcggtc ttaactttat
     4201 gtttctagga aaagtcgtcg gcacggtctg gtcaacaaag aagacgcctg atctcgaagg
     4261 cgtcaaattt ctgatcgtgc atccgattga gttggacaaa gagccgaacc gcagcattat
     4321 cgtggtggcg gatcgtctgg gggcaggcgt gggcgagatc gtgatgtgcg ccttcgggaa
     4381 ggcggcgcgc tcggccatcg gcgatcagga gatgtcgatt gaggcggcgg tcgtcggcat
     4441 cgtggatcgc gtggacgtgc aaggctcgcc ctctgacgac ctcaacgaaa ccgcacacga
     4501 attgagaagc ggagacaacg gcagaagatg aaacagtgac aggtgacgag aaagaaaagt
     4561 gatgagtgat gagtaatgag taatgagtga aagacaagtc tttggttctg actcatcgcg
     4621 catcacccat cattcattac ttcatcctcg tcacccgtca ctcgttactt aaaagaatgg
     4681 caaacgagga attgatctac aggatcacgc gggcggtcta cgggcgtctg ggggcgcagg
     4741 ccgatgagcg cacggtggag aatttggtga cggacatcta ccgcgcgatt gagccggtgg
     4801 tggaagcacc ggcggacggc gacaatggcg caacgcagcg cggcgcacgt cgcggcctga
     4861 ccgcgcacgg cggcgaggcc gaaggctctt cggatcgtct catcgtgtcg gtcttcggcg
     4921 tggatcatcc gggcatcgtg gcgggcgtgg cgcagattct cgcagagtcc gggtgcagca
     4981 tcgcggacat caaccagacg gtggtgcagg gcaagtttgc gatggtcatt atcgccgatt
     5041 cgtcaaactc gaacgcaccc gtctcgacac tcaaggaacg cttccgcgcc gctggagaca
     5101 aactcggcgt tcgcatctac gcccagcgcg aagacctctt caacgcaatg catagaatct
     5161 gaagtgatga gtgagaggtg atgagtgatg agtcaaatcc ggcttctcac tcatcac
//
LOCUS       DASWNS010000307         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_141262, whole genome shotgun sequence.
ACCESSION   DASWNS010000307 DASWNS010000000
VERSION     DASWNS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141262"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VGB76_10855"
     CDS             <1..773
                     /locus_tag="VGB76_10855"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEY0099436.1"
                     /translation="FITHMHGDHLFGLMGLLATCGLAGQAQEIHVYGPDGLEDYIRMS
                     GQLSRTTISDNLNIHTVEPGRIYYDDDFSVTCQPLRHRITSYGYRVTESDRPGKFDVE
                     RAHALGVPAGPLFGRLKRGESVELPDGRIVHGRDFDSPLIEGRSIVYCTDTTYCRSAV
                     ALSEGADALIHEATFAEQDEPLALQSIHSTTTMAARVASEARVGQLLLTHFSPRYTKE
                     SEITPDDLLAQARAIFPRTEMARDFMTYEVPRRDRSAG"
     gene            1025..>1659
                     /locus_tag="VGB76_10860"
     CDS             1025..>1659
                     /locus_tag="VGB76_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099437.1"
                     /translation="MSRQTPPRNDRFEDYVPVAERLEKFYERFPDGRVITNIIEHNLE
                     SGFVLMRAEVYRSPDDTQPAATGHAFEVRGESYVNKTSYIENCETGAVGRALALLGFE
                     VKRGIASREELEKSARMTPEKVARAASVVDASASVPVKPRVEAGEQGMSLDAEILQSA
                     GALGYDAAKVRKWIDQKFKVTGGLEALSEEKKREVLRVFREKASPAAAKGTT"
BASE COUNT          374 a          468 c          518 g          299 t
ORIGIN      
        1 tcttcatcac acacatgcac ggcgatcatc tcttcggctt gatgggactg ctcgccacct
       61 gcgggctggc gggacaagcg caggagatac acgtctatgg tccggacggc ctcgaagatt
      121 acatacgcat gtccggccaa ctctcgcgca cgaccatctc cgacaatctc aacatccaca
      181 cggtcgaacc cggtcgcatc tactacgacg atgatttcag cgtcacctgc caaccgctca
      241 gacatcgcat cacgtcctac ggctaccgtg tcacggagag cgacaggccg ggaaaattcg
      301 atgtggaaag ggcgcacgcg ctcggcgtcc ccgcagggcc tctgtttggt cgcctgaagc
      361 gcggcgagag cgtcgaactg cccgacgggc gcattgttca cgggcgcgac ttcgacagcc
      421 ccttgatcga agggcgttca atcgtctatt gcaccgatac gacttattgc cggagcgcgg
      481 ttgcgctctc cgagggagca gacgctttaa ttcacgaagc gaccttcgcc gagcaggatg
      541 agccgctcgc cctccagagc atacactcga cgacgacgat ggccgcgcgc gtggcaagcg
      601 aggcgcgcgt cggtcaattg ctgttgacgc atttcagccc gcgttatacg aaagagagtg
      661 agatcacacc ggacgatctg ctggcacagg ctcgcgccat atttccccgc acagagatgg
      721 cgcgcgactt catgacctac gaagtcccac gccgcgaccg gagtgcgggg tgagagttga
      781 agggcgaact tgaggcgggc gacgtacgct gtgcaccagt cagtcgcgcg gcgggcgagt
      841 ttgtaaccgg cgaactcctt gtttgtaaaa aagcattttg aaggttagat tgtgtccgca
      901 tgaccggact agaagttaaa cggaggccga cgcgctgtag tgccagccga cttcgacacc
      961 ggcacaggtt attccccacg cctctgagct aaatttgtca aaacccgaag gagtgagaaa
     1021 cgccatgtcg cgccaaacgc cgccgcgtaa cgatagattc gaggattacg ttcccgtcgc
     1081 tgaacggctg gaaaaatttt atgagagatt ccccgacggg cgcgtcatca caaatatcat
     1141 cgagcataat ttggagagcg gcttcgtcct gatgcgcgcc gaagtctatc gctcgcccga
     1201 cgacacgcaa cctgcggcca ccggccacgc ctttgaagtc cgcggcgaga gttatgtgaa
     1261 taagacgagt tacatagaaa actgtgagac aggcgcggtc gggcgcgcac tggctctgct
     1321 cggctttgaa gtgaagcgcg ggatcgcgag ccgtgaagaa ctcgaaaaga gcgcgcggat
     1381 gacgccggag aaggttgcgc gtgcggcgag cgtcgtagac gcttcagcga gtgtgcctgt
     1441 gaaaccgcgt gtcgaagcgg gagagcaggg catgagtctc gacgcggaaa ttctgcaaag
     1501 cgcgggcgcg ctcggctacg atgcggcaaa ggtgcgcaag tggatcgatc agaagtttaa
     1561 ggtgacgggc ggccttgagg ctttgagcga agagaaaaaa cgcgaggtgc tgagagtgtt
     1621 tcgcgagaag gcttcaccgg cagcggcgaa ggggacaac
//
LOCUS       DASWNS010000308         4492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_374963, whole genome shotgun sequence.
ACCESSION   DASWNS010000308 DASWNS010000000
VERSION     DASWNS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374963"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VGB76_10865"
     CDS             complement(<1..1233)
                     /locus_tag="VGB76_10865"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEY0099438.1"
                     /translation="MANKDDNDIDLNPIPDFPYDTDVVEREGWASQLGLVLAMAGNAV
                     GLGNFLRFPALAARNGGGTFMIPYFIAFLLLGIPLMWIEWGIGRFGGKYGYSTSPGMF
                     EKMWANPLSKYLGALGVFIPLAFVIFYTYIESWTLGYAVLSFANQMPSIAGQSPDQAF
                     TTMGDYLAAYQGTNNGLAWSGSVVGVIFFAITLFLNIFIISKGIAGGIERMAKIGMPL
                     LILFAIIVGIRVLSLPGAMGGIAFVWNPDFSRITDGAVWLAAAGQIFFTLSLGTGSIQ
                     TFASYLKETDDIVLTGLATSSMNEFCEVILGGSIAIPAATAFYGAGAATAIANSGTFN
                     LGFQTMPAVFSGLPGGFLFGGIWFTLLFFAGITSSLALAQPAMAFLQDELRMTRQKAA
                     MVVGAIIFVAVLPIIMFLQ"
     gene            complement(1320..2939)
                     /locus_tag="VGB76_10870"
     CDS             complement(1320..2939)
                     /locus_tag="VGB76_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0099439.1"
                     /translation="MAKNHQYFSRKPLDMLLEEAKGENRLRRVLGPVALSSLGVGAII
                     GAGIFVSTGAIARQTAGPALMLSYVAAGLVCIFAALCYAEFASMVPVAGSAYTYAYAT
                     LGELFAWIIGWDLVLEYAVGSAVVANGWSGYFQSVLSLLGITLPKALSGPVLQYSATS
                     NNFMHLEPTGSLINLPAVLIVALVTIVLVVGIKESTRFNATMVVVKLAAVLFVIGVGA
                     FYINTANWTPFAPYGLTGLSFFGTPIAGQVDAAGRPLGMLAGAALAFFAYIGFDSVST
                     HAEEAKNPQRDVPIGIVASLVICTVLYIAVVAVLTGMVHYSQLDINAPVSEAFKQVGL
                     PWAQGLIALAGVAGITSVLLVMMLSQPRVLLAMARDGLIPASFFGAVHEKFRTPYKST
                     ILTGIFVGILAAFLPIDVLLELVNIGTLMAFVIVCAAVLIMRKKHPEAERPFRAPFVP
                     LVPILGIVTCSILMFSLPAYNWVRLAIWLGLGFVIYYLYGRKHSVMSKYNLAHELGTH
                     GISPGGQAVGDPNAPPDSPARPIDTEVKKDK"
     gene            complement(3093..>4492)
                     /locus_tag="VGB76_10875"
     CDS             complement(3093..>4492)
                     /locus_tag="VGB76_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0099440.1"
                     /translation="VGSPGWLLVVWIITGLLTLMAALSYGELAAMMPKAGGQYVYLRE
                     AFSPMWGFLYGWTLFLVIQTGTIAAVAVGFARYTGVLVPWVSEKNYLIEPVRLGGYAL
                     SLSTAQLVGLLMIALLTFMNTRGLKLGKLVQNVFTVAKTGALIALIVLGIIVGLNSGA
                     GTANFSNFWEVRGNLQEVGQGLTAAAAFGLFVGICVAQTNSLFSADAWNNITFTAGEV
                     KNPRRNIPLSLALGTILVIALYLLANVAYLVALPFEAIQNAPSDRVASETANVIFPGF
                     GATVMAVAIMVSTFGCNNGLILAGARAYYAMARDGLFFRAAGELNENRVPAWGLVIQG
                     VWAALLVLPRTVSTDAAGAIAYGNLYGNLLDYVISAALIFYILTIIGIFVLRRKRPDV
                     ERPYKAFGYPVVPALYIVGASIILVVLFIYQTATTWPGLLIVLTGIPVYFLWRKIGVP
                     MAADPREDDAMAQGI"
BASE COUNT          948 a         1315 c         1467 g          762 t
ORIGIN      
        1 ttgcaggaac atgatgatcg gcagcacggc gacgaagatg atcgcgccga cgaccatcgc
       61 cgccttctgt ctcgtcatac gcagttcgtc ttgcagaaag gccatcgcgg gttgcgcgag
      121 cgcgagcgac gaggtgatgc ctgcgaagaa gaggagcgtg aaccagatgc cgccgaaaag
      181 gaagccgccc ggcagaccgg agaagacggc gggcatcgtc tggaagccga gattgaacgt
      241 gccggagttg gcgatggccg tggccgcgcc cgcgccgtaa aaggccgtcg ccgccgggat
      301 ggcgatggaa ccgccgagga tgacttcgca aaactcgttc atcgaggaag tcgccaatcc
      361 cgtcagcacg atgtcgtcag tctctttcag gtaactcgca aaagtttgaa tcgaacccgt
      421 gccgagcgag agcgtgaaga agatctgacc ggcggcggcg agccagaccg cgccgtccgt
      481 gatgcgcgag aagtcggggt tccagacgaa ggcgatgccg cccatcgcgc cgggcagcga
      541 gaggacgcgg atgccgacga tgatcgcgaa gaggatcagg agcggcatgc cgatcttcgc
      601 catgcgctcg atgccgcccg cgatgccctt cgagatgatg aagatgttga ggaacagcgt
      661 gatggcgaag aagatgacgc cgacgacgct gcccgaccac gccagcccgt tgttcgtgcc
      721 ctgataggcg gcgaggtagt cgcccatcgt ggtgaacgcc tgatcggggc tttgcccggc
      781 gatgctcggc atctggttgg cgaaggagag cacggcgtaa ccgagcgtcc atgattcgat
      841 gtaggtgtag aagatgacga aggcgagcgg gataaagacg ccgagcgcgc cgaggtactt
      901 cgagagcggg ttcgcccaca tcttctcgaa catgccgggc gaggtcgaat aaccgtactt
      961 gccgccgaaa cgcccgatgc cccattcgat ccacatcaga gggatgccga gcagaaggaa
     1021 ggcgatgaag tacgggatca tgaacgtccc gccgccgttg cgcgccgcga gcgcggggaa
     1081 gcgcaggaag ttgcctaagc ccacggcgtt gccggccatc gccagcacga gtccgagttg
     1141 cgatgcccag ccttctctct cgacgacatc cgtgtcgtaa gggaaatcag ggatcggatt
     1201 caggtctata tcgttgtcgt ctttgtttgc catatcaaaa gtacgctctt ctttggcctt
     1261 gaaggttgag tcaccgtacg gttcgagacg gcgactcaac tcctgtcgtc taatttacac
     1321 tatttgtctt ttttcacttc cgtgtcaatc ggtcgtgccg ggctgtcggg cggcgcgttc
     1381 gggtcgccaa ccgcttgacc gccgggcgag atgccgtgcg tgccgagttc gtgcgcgaga
     1441 ttgtattttg acatcacgct gtgcttgcgt ccatagagat agtagatgac gaagccgagg
     1501 ccgagccaga tggcgaggcg cacccagttg taagcgggca gcgagaacat caggatggag
     1561 cacgtcacga tgccgaggat cggcacgagc ggcacgaacg gcgcgcggaa cgggcgttcc
     1621 gcctccgggt gtttcttgcg catgatgagc accgccgcgc agacgatgac gaaagccatc
     1681 agcgtgccga tgttgacgag ttcgagcagc acgtcgatgg gcaggaacgc ggccagtatg
     1741 ccgacgaaaa tgccggtcag gatcgttgac ttgtagggcg tgcgaaactt ctcatgcacc
     1801 gcgccgaaga aactcgcggg gatgagtcca tcgcgcgcca tcgccagcag cacgcgcggc
     1861 tggctgagca tcatcacgag cagcacggag gtgatgcccg ccacgcccgc cagcgcgatc
     1921 agaccttgcg cccacggcag cccgacctgt ttgaaggctt ccgaaaccgg cgcgttgatg
     1981 tccaactgac tgtagtgcac catgccggtc aggacggcga cgaccgcgat gtagagcact
     2041 gtgcagatca cgagcgaggc gacgatgccg atgggcacgt cgcgctgcgg gttcttggcc
     2101 tcctccgcgt gggtcgaaac cgagtcgaaa ccgatgtagg cgaagaaggc gagcgccgcg
     2161 cccgcgagca tgccgagcgg tctgcctgcc gcgtccacct gaccggcgat gggagttccg
     2221 aagaagctca agcccgtcag accgtaaggg gcgaacggcg tccagttcgc cgtgttgatg
     2281 tagaacgcgc cgacgccgat gacgaagagc acggcggcga gtttgacgac caccatcgtc
     2341 gcgttaaagc gcgtgctctc cttgatgccg acgacgagca cgatggttac cagcgcaaca
     2401 atgaggacgg cgggcaggtt gatgagcgaa cccgtgggtt ccagatgcat gaagttgttg
     2461 ctggtggcgc tgtattgcag caccggcccg ctcagggctt ttggcagagt tatccccagc
     2521 agactcagca cggattgaaa ataacccgac cagccgttag cgaccaccgc cgacccgacc
     2581 gcgtattcca agaccagatc ccaaccgatg atccacgcga acagttcgcc gagcgtggcg
     2641 taagcgtagg tgtaggccga acccgcgacc ggcaccatcg aggcgaactc cgcgtagcag
     2701 agcgcggcga agatgcagac cagaccggcg gcgacatacg agagcatgag cgcgggtccg
     2761 gccgtctgcc gtgcgatggc tccggtcgag acgaaaatgc ccgccccgat gatcgcgccc
     2821 acgccgaggc tggagagcgc gaccgggccg agcacgcggc gcagacgatt ctctcccttc
     2881 gcctcttcca acagcatgtc gagcggtttt cttgaaaaat attgatgatt ttttgccatg
     2941 taagtcgaac ctccgcgaat tttacggtag ttttaagttg atgaacggag caatcacctg
     3001 tctcctaaac gtgcttgaaa agagtctgcc ctttgaaatt tcagggcggt gccgaatcaa
     3061 attccggcga gtcacaggta aagttccttg aactaaatcc cttgcgccat cgcgtcgtct
     3121 tcgcgcgggt ctgcggccat aggcacgccg attttgcgcc agaggaaata gaccgggatg
     3181 cccgttagca cgatcaacag acccggccac gtcgtcgccg tctggtagat gaacaggaca
     3241 actaaaatta tggacgcgcc gacgatgtag agcgcgggga cgaccgggta gccgaaggct
     3301 ttgtaaggcc gctccacgtc aggacgcttt cggcgcagca cgaagatgcc gatgatcgtc
     3361 aggatgtaga agatgagcgc ggcgctgatc acgtagtcga gcagattgcc gtagaggttg
     3421 ccgtaagcaa tcgcgcccgc cgcgtccgtg ctcaccgtgc gcggcaggac gagcagagcc
     3481 gcccagacgc cctgaatgac gagtccccac gcgggcacgc ggttttcgtt caactcgccc
     3541 gccgcgcgga aaaacagccc gtcgcgcgcc atcgcgtagt aggcgcgtgc gcctgcgagg
     3601 atgagtccgt tgttgcagcc gaaggtcgaa accatgatcg ccacggccat gacggtcgcg
     3661 ccgaaaccgg ggaagatgac gttggcggtt tcggaagcga cgcggtcgct cggcgcgttc
     3721 tggatcgcct cgaaggggag cgcgacgaga taggcgacgt tggcgagcag gtagagtgca
     3781 ataacgagga tggtgccgag cgcgagcgag agcgggatgt tgcggcgcgg attcttcacc
     3841 tcgcccgccg tgaaggtgat gttattccag gcgtctgcgg agaagagcga attggtctgc
     3901 gcgacgcaga ttccgacgaa gaggccgaag gccgccgcag ccgtcaagcc ctgccctacc
     3961 tcttgcaggt tgccgcgcac ttcccagaag ttcgagaagt tggccgtgcc cgcgcccgaa
     4021 ttcaacccga cgatgatgcc gagcacgatg agcgcgatga gcgcgcccgt cttcgccacc
     4081 gtgaagacgt tttgcacgag tttgccgagt ttcaggccgc gcgtgttcat aaaggtgagc
     4141 agcgcgatca tcaacagccc gacgagttgc gccgtcgaaa gcgagagcgc atagccgccg
     4201 agccggacgg gttcgatgag atagtttttc tccgacaccc acggaacgag tacgcccgtg
     4261 tagcgcgcga agccgacggc gaccgccgca atcgtccccg tctggatgac gagaaagagc
     4321 gtccagccgt agagaaagcc ccacatgggc gagaacgctt cgcgcaggta gacgtattgc
     4381 ccgcccgctt tcggcatcat tgcggccagt tcgccgtagg agagcgcggc catcagcgtg
     4441 agcagcccgg tgatgatcca gacgacgagc agccagccgg gcgagccgac ct
//
LOCUS       DASWNS010000309         4527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_401129, whole genome shotgun sequence.
ACCESSION   DASWNS010000309 DASWNS010000000
VERSION     DASWNS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401129"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..2413
                     /locus_tag="VGB76_10880"
     CDS             128..2413
                     /locus_tag="VGB76_10880"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY0099441.1"
                     /translation="MNTQMLLIFLISFAALGFAFLLIKNVLDRDTGTPAMRDISDAIK
                     EGAEAFLKRQNTTIAILAIALALLIFIVYGFVRSHNAADPIDSVGLAFWTTFSFVLGA
                     ASSVASGYIGMWVSIRANIRTASAATRSLNGALQTALRGGAVSGLFVVALSLLGVAIL
                     FELVDRLTEVPATKIPLLIAGYGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAGI
                     PEDDPRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILAAALYSSNPDFFTPG
                     QLLGIILFPLVARAFGLVASIFGIMIVRTREEGDPMAALNRGYFLTSALAAVGFLVAC
                     YWLLRPAGANAAAAPNAWLYFFLCGLIGIATAILFVFITQYYTEYRYRPVREIAEASQ
                     TGPATNVISGVAVGLESTAFPILVIAAAIISSYYLGTASGLNNAGFFGTAVATMGMLG
                     TAAYILAMDTFGPITDNAGGIVEMSQQPEEIRRKTDRLDAVGNTTKALTKGYAVGSAA
                     LAAFLLFSAYLDEVKNYGLNLGTGGLGSAEAGRGAELFVINLAKPEVFIGGLLGAMLV
                     FLFSSLAIKAVGKAAYEVIRDVRAQFKENPGIMEGTSKPNYGRTVDIVTRGALKQMIL
                     PGVLAVGLPIAVGLIFRALFYAQIGTGDRTNSVASLVGNPRYAHLATGAEVVAGMLMI
                     GTIVGILMALFLNNAGGAWDNAKKYIETGEYGGKRSEAHRAAVVGDTVGDPFKDTAGP
                     SLHVLIKLLSTITLVLAPLFI"
     gene            2510..3097
                     /locus_tag="VGB76_10885"
     CDS             2510..3097
                     /locus_tag="VGB76_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEY0099442.1"
                     /translation="MNTSYAKAETLTGAWLAPVDAVKSVALVIAFSLLIALSAQVVIP
                     LPFTPVPITLQTFAVLLTGALLGSRLGALAVVAYLVEGASGLPFFRGGNGGWLYLAVS
                     PTAGYLLAYPLAAFLTGWLAERGWDKRFLTAAAAIGLGSIVILFGGWLGLLRFYSAPQ
                     AFALGVVPFIIGDIVKIALAAAALPSGWALLRRKG"
     gene            3193..>4527
                     /locus_tag="VGB76_10890"
     CDS             3193..>4527
                     /locus_tag="VGB76_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY0099443.1"
                     /translation="MKQASKKALARSKASVPCNEAERLAALHECAILDTDAEESFDDI
                     TRLAAYICATPIALISLVDTSRLWFKAKVGFAPSELPRASTFCTQAILKPEPFIIEDA
                     SADKRFAKNPYVMSAPHVRFYAAVPLVTPDGHALGVLCVIDHVARTIDDEQLEALHVL
                     ARQTMTQLELRRNSNTLAELNRKLDREIEERKRAEAERVELLAREQELRGVAEANEQR
                     YRFLAESIPQQVWTAQPDGRLDYVNRRTIEYFSRSEDEITGESWQQVIHPDDLSACLA
                     RWEQSLKSGEIYEVEFRLRHGADGDYRWHLGRALPMLDTDGRIIRWFGTNTDIDDQKR
                     LYRLAEEANRAKDAFLATVSHELRTPLTSMMGWAELLKLGMLDEKGQRHALDVIESSA
                     RAQAQLIGDLLDISRIISGKLRLEVQPVELPPIIEAAMDVVHPAAEAKSIQLV"
BASE COUNT          809 a         1458 c         1350 g          910 t
ORIGIN      
        1 agttcgttgc gaactcagga gcggtctggt ttaactgtcc ctgctagtcg ttcgatcttt
       61 tcagccgtcg tttgttcacg caaacggcgg ctgaattcta tttgcacgag agaaagggtg
      121 acctaacgtg aacacacaaa tgctcttgat ctttctgatc agctttgccg cgctcggctt
      181 cgccttcctg ctcatcaaga acgtgctcga ccgcgacacc ggaacgcccg ccatgcgcga
      241 catctccgac gcgatcaagg agggcgcgga agcgtttctc aaacggcaga acacgaccat
      301 cgccattctc gccatcgcgc tcgccctgct catcttcatc gtctacgggt ttgtgcgctc
      361 gcacaatgcc gccgacccga ttgactccgt cgggctggcc ttctggacca cattctcctt
      421 cgttctcggc gcggcctcct cggtggcctc cggctacatc ggcatgtggg tttccattcg
      481 cgccaacatc cgcacggcgt ctgccgccac gcgctcccta aacggcgcgc tccagacggc
      541 cttgcgcggc ggcgcggttt ccggcctctt cgtcgtcgcg ctctcgctgc tcggcgtcgc
      601 catcctgttc gaactcgttg accgcctgac ggaagttccg gcgacgaaga ttccgctcct
      661 gatcgcgggc tacggtttcg gcgcatcgtt cgtcgcgctc ttcgcccaac tcggcggcgg
      721 catctacacg aaggcggcgg atgtgggcgc agacctcgtc ggcaaagtcg aagcggggat
      781 accggaggac gacccgcgca accccgccgt gatcgcagac ctcgtgggcg acaacgtagg
      841 cgactgcgcg ggtcgcggcg cggacttgtt cgagtcaacc gccgccgaaa acatcggcgc
      901 gatgatcctc gccgccgcgc tctacagttc aaatccagac ttcttcacgc ccggccaact
      961 gctcggcatc attctctttc cgctcgtcgc gcgtgccttc ggcctcgtcg cctcgatctt
     1021 cggcatcatg atcgtgcgca cgcgcgaaga gggcgacccg atggccgcgc tcaatcgcgg
     1081 ctactttctg acctccgcgc tcgccgccgt cggcttcctc gtcgcctgct actggctgct
     1141 ccgaccggcg ggcgcgaatg cagcagccgc gcccaatgcc tggctctact ttttcctctg
     1201 cggtttgatc ggcatcgcaa cggcgatcct cttcgtcttc atcacgcagt attacaccga
     1261 atatcgctat cgccccgtgc gcgagatcgc ggaggcttcg cagaccggac ctgcgacgaa
     1321 cgtgatcagc ggcgtcgccg tcgggttaga gagcacagcc tttccgatcc tcgtgatcgc
     1381 cgccgcgatc atcagttcgt attatctcgg cacggcttcc gggctgaaca atgcgggctt
     1441 cttcggcacg gcggtcgcca cgatgggcat gctcggaacc gccgcctaca tcctcgcgat
     1501 ggacaccttc ggacccatca cggacaacgc gggcggcatc gtcgagatgt cgcagcagcc
     1561 ggaagagatc aggcgtaaga ccgaccgcct cgatgccgtc ggcaacacga cgaaggctct
     1621 gacgaaaggt tacgccgtcg ggtcagccgc gctcgccgcc tttctcctgt tctcggccta
     1681 tctcgacgaa gtgaagaact acggcctcaa tctcggcacg ggcgggttgg gttccgcgga
     1741 agcggggcgc ggcgcggaac tcttcgtgat caatctggcg aagccggaag tcttcatcgg
     1801 cggactgctc ggcgcgatgc tcgtcttcct cttctcgtcg ctggcgatca aggcggtggg
     1861 caaggcggct tacgaagtga tccgcgacgt gcgcgcgcag ttcaaagaga atccgggcat
     1921 catggagggc acgtcgaagc cgaattacgg gcgcacggtt gacatcgtga cgcgcggcgc
     1981 gctcaagcag atgatcctgc cgggcgtgct cgccgtcggc ctgcccatcg ccgtcggcct
     2041 catcttccgc gcactcttct acgcgcagat cgggacgggc gaccggacga acagcgtcgc
     2101 ctcgctcgtc ggcaacccgc gttacgcgca tctggcgacg ggcgctgagg tcgtcgcagg
     2161 gatgttgatg atcggaacca tcgtcggcat cctgatggcg ctctttctca acaacgcggg
     2221 cggcgcgtgg gacaacgcca agaagtatat cgagacgggc gagtatggcg gcaagcgcag
     2281 cgaggcgcac cgggcggcgg tcgtgggcga cacggtcggc gatccgttca aagacacggc
     2341 ggggccttcc ctccacgtct tgatcaaact gctctcgacc attactcttg tgctcgcacc
     2401 attgtttatt taactacagt gacaagtgac aagtgacaag tgacaagcaa aagcgtcttg
     2461 tcttaaactc gtctctcatc gcctgtcact catcactaag gttgaaacta tgaatacatc
     2521 ttacgcaaaa gctgaaactt tgacgggcgc gtggcttgcg cctgtggacg ctgtgaagtc
     2581 ggtcgcgctc gtcatcgcgt tcagtctgtt aatcgcgctc tcggcgcagg tggtcattcc
     2641 tctgccgttt acgcccgtgc cgatcacttt gcagaccttc gcggtcttgc tgacgggcgc
     2701 gcttctggga tcgcgtctcg gtgcgctcgc ggttgtggct tatctcgtcg aaggcgcgtc
     2761 ggggctgccg ttctttcgcg gcggcaacgg cggctggctc tatctcgcgg tcagcccgac
     2821 agccggttac ctgctggctt acccgctggc agcgtttctc acgggctggc tcgcggagcg
     2881 cggttgggac aagagatttc tgacggcggc agcggccatc gggcttggct caatcgtgat
     2941 cttattcggc gggtggctcg gactgttgcg cttctattct gcgccgcaag cgttcgcgct
     3001 cggcgtcgtg ccgttcatca tcggcgacat cgtgaagatc gcgcttgcgg cggcggctct
     3061 gccttcgggc tgggcgttgc tgcgaagaaa gggatgaagg atgagggatg agggatgaat
     3121 cagaaaaagc actcaacctc ttgaatgctt tttctgattc gtccctcatc cttcatctct
     3181 ctgtttcatc ctatgaagca agcaagcaaa aaagctctgg caagatcgaa agcgtccgtc
     3241 ccttgtaatg aagcggagcg gctcgccgcg ctgcacgagt gtgccatcct cgacaccgac
     3301 gccgaagagt ctttcgacga catcacgcgc ctcgccgcct acatctgcgc gacgcccatt
     3361 gcgctcatca gtctggtgga cacgtcgcgc ctgtggttca aagcgaaagt cgggttcgcc
     3421 cccagcgagt tgccgcgcgc gtccaccttc tgcacgcagg ccattctcaa gcccgaaccc
     3481 ttcatcatcg aagacgcttc ggcggacaag cgtttcgcca aaaaccctta cgtcatgtcc
     3541 gccccgcacg tccgctttta tgcggcggtg ccgctcgtta cgcccgacgg gcacgcgctc
     3601 ggcgtgctct gcgttattga tcacgtggcg cgcacgattg acgacgaaca gcttgaggcc
     3661 ttgcacgtgc tcgcgcgcca gaccatgacg caactcgaac tgcgtcgcaa ctcgaacaca
     3721 ctggccgaac tgaatcgaaa actggacagg gagatcgaag aacgcaagcg cgccgaggcc
     3781 gagcgcgtgg agttgctggc gcgcgagcag gagttgcgcg gcgtggcgga agccaacgag
     3841 cagcgttatc gctttctcgc cgaatccatc ccgcagcagg tttggacggc gcagcccgac
     3901 ggcaggctcg attacgtcaa tcgacgcacc atcgaatact tctcccgcag cgaagacgag
     3961 atcacgggtg agagttggca acaggtgatt cacccggatg atctctccgc gtgcctcgca
     4021 cgctgggaac aatcgctcaa gtcgggtgaa atctatgaag tggaattccg gctccggcac
     4081 ggcgcagacg gagattatcg ctggcactta ggccgcgcgc tgccgatgct tgacacggac
     4141 gggcgcatca ttcgctggtt cggcacaaac acggacatcg acgatcaaaa acgcctctac
     4201 cgtctcgcgg aggaggccaa ccgcgcgaag gacgcttttc tcgccaccgt ctcgcacgaa
     4261 ctgcgcacgc cgctcacttc gatgatgggt tgggcggaac ttctcaaact cggcatgctc
     4321 gacgagaaag gtcagcggca cgcgctcgac gtgatcgaat cgagcgcgcg tgcgcaggcg
     4381 caactcatcg gcgatctgct cgatatctcg cgcatcatct ccggcaaact ccggctcgaa
     4441 gtgcaacccg tcgaactccc gcccatcatc gaggcggcga tggacgtggt gcatccggcg
     4501 gcggaagcca agtcgatcca actcgtc
//
LOCUS       DASWNS010000310         8261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_480775, whole genome shotgun sequence.
ACCESSION   DASWNS010000310 DASWNS010000000
VERSION     DASWNS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480775"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /gene="proS"
                     /locus_tag="VGB76_10895"
     CDS             complement(<1..1003)
                     /gene="proS"
                     /locus_tag="VGB76_10895"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY0099444.1"
                     /translation="MRWSNYFIPTLREVPADAEVVSHRLLLRAGMVRQLAAGIYSYLP
                     LSWLVMRKVMRVVREEMDAIGGQEFFLPALNPREIWEETGRWTVMGDNMFRLKDRKGA
                     DLCLGMTHEEIFTGIARHELRSYKQLPQVWYQIQTKFRDEARPKSGVLRVRQFIMKDA
                     YSFDVDSAGLDKSFEDQRAAYIKIFTRCGLKFSIVEASSGAMGGTASNEFMVKSDAGE
                     DLIATCEACGYAANLEKATSRIAAISDPAAATGAPEEFPTPGVRTIEDLITFPGGAAA
                     DRQVKTLVYIATNETGENFPALALLRGDHQLHETKLSDTLGAIAVRPAHPEEIRDLLG
                     "
     gene            1186..1653
                     /locus_tag="VGB76_10900"
     CDS             1186..1653
                     /locus_tag="VGB76_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099445.1"
                     /translation="MSEKLRVSFNSPQCGWMSFELRAGEQSLIDAVSYTPYDSLHDLI
                     RALSKLLVDDTELTVKWAYNPDELDFNFRASGDEAELEVKWYKNHLRVAGTGERRFFF
                     QGSRLDLCHPFWKALRDIQSDTEVDEFTRNWRREFPEAEMKRLTEEIKAFKLK"
     gene            1778..3088
                     /gene="glyA"
                     /locus_tag="VGB76_10905"
     CDS             1778..3088
                     /gene="glyA"
                     /locus_tag="VGB76_10905"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY0099446.1"
                     /translation="MTDRRHNAPLAEVDPLINDAINHEVARQAEGLELIASENFVSEA
                     VLEAMGSVFTNKYAEGYPKKRYYGGCEFADVVEQAAIDRAKELFGAEHANVQPHSGAQ
                     ANMSVYLAAIQYGDTILGMNLSHGGHLTHGHPMNFSGISYKVADYGVSPETEQIDYDD
                     LARRAEEHRPKMIVCGASAYPRTIDFARIGEIARSVGARVMADIAHIAGLVAAGLHPS
                     PVPHADYVTTTTHKTLRGPRGGLILCREEHAAEVNKKLFPGVQGGPLVHIIAAKAVAF
                     GEALGADFREYQRQVVLNASALAEELKAQGFRLVSGGTDNHLVLVDVFMGGAGITGKV
                     AEKALEAANITVNKNTIPFDTNKPFVTSGVRLGTPALTTRGMGEGEMKTVARLIAEIL
                     HAPESEEVRQKVRREVAELAARFPLYPNRLRHTTPPEVEAMGAD"
     gene            3162..4628
                     /locus_tag="VGB76_10910"
     CDS             3162..4628
                     /locus_tag="VGB76_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099447.1"
                     /translation="MKEPERLLAALRFCCYAAAVLVPFYLLIRANSAWTPHPRFFPDD
                     AVYARMCWPSGFIVYWHDFGFYRLLAVPAYALSNRAVMRASLSPVPFTLIASAFATLV
                     FVWTCVSVGLSRRRGVLLAGVLLSSPLLLETLNFWSGTFNYALVLLLLAAQLRACVWA
                     RRRFVGRPSRGYLVTACGALIALLTYEIALPFILATSAFYTRRGRARELLSVCIACAA
                     LLAFVGALVWAGVYWPLRFRLMAEQLASAVSGTPAPNLHAPSPATATPLSTLGRAQMY
                     ASLLFSFVRTGLLSPSFWGLAGALAGLGFLARTPTEKASHPAAARETEAQPPDDAEAQ
                     LPDDKEGRLPDDKEGRLPRDLVALGFAFSFGACVVYLLVTRSLNARYVAFLLIYGAAT
                     LAWTKWRAASFALASLLLLQAAVAASLPVHMRDVELAAKGEINAARTGRGDLISVDGR
                     LTRGSWGKRFFAPEPIDLLKQPLNSRACRYAAPCEECR"
     gene            4735..5421
                     /locus_tag="VGB76_10915"
     CDS             4735..5421
                     /locus_tag="VGB76_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099448.1"
                     /translation="MKTSPKFCRHALALLFAVTIGTATAQAQKQAEEKIILPPSPAPP
                     IAQPLPTPLQAKPVADKLAPAGWMRYEVGEPLVFSLLLPTQPMGMVDRVNVAPGVEGI
                     SRIYLSVADTGVYGVSFMDALPAELLTEPRRRTFFEGFSQEFAEGFQARMKERGMEVR
                     LTMLEQRAASASGMTGYEQDFSYGQVMGRVRVVFGAGSAYAVMAIWNGLSLNSERSAF
                     FESLKVSAKR"
     gene            5463..7433
                     /locus_tag="VGB76_10920"
     CDS             5463..7433
                     /locus_tag="VGB76_10920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY0099449.1"
                     /translation="MEEIFGRNGLISRSHPDYEFRPGQIQMAEAVLRAFEERRHLLVE
                     AGTGTGKTLAYLVPAIAAATARGERVIISTGTKNLQEQLMEKDIPFLQRVLPKKFTAA
                     YMKGRNNYACLQRIKRAESAPILDGLDDIDHFEEVRRWARDSQTGDRAELVQLPENLS
                     FWRHIDARSEICMGQKCPDYDPCFITRMRQRAQDADIVIVNHHLFFADLALRGGEYGQ
                     VLPDYSAVIFDEAHSIEDVAAEYFGHQVSSYQIEDLLRDLQILPVTDVDANKELTRAG
                     GRVARFSERFWQGFQERGEEGRAPILPGTFARKNPRGEIEATALGDSYLSLDGALNRM
                     ETTLDALREQPPEVENLIRRIREVRFGLEFIVAGDDKRYVYWMERRGRGMFLRASPID
                     VSTLLQDKLFERVETVVMTSATLASAGSFTFARERLGLNDEKTDELIAPSSYDYQNQS
                     VLYLPPRMPDPRSPQWAEAAAAEVLRILEVTDGRAFVLSTSLSGMRALYERVAPQVDF
                     PCFMQGTAAKSALLDKFRTTPNAVLFATSSFWQGVDVRGDALSCVIIDKLPFAVPSDP
                     IVAARQRFIEDAGGSSFYEYSVPQAIISLKQGLGRLIRSATDRGVLSVLDPRLRTQPY
                     GRQFIKSLPPCRVTSDLKEVARVFVESAAAVR"
     gene            7496..7696
                     /locus_tag="VGB76_10925"
     CDS             7496..7696
                     /locus_tag="VGB76_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HEY0099450.1"
                     /translation="MSKDASEDKTIYKVVVNRDQYSIWPAHRENARGWTDAGQSGTKQ
                     QCLTYIEKVSKAARPPAEKKQR"
     gene            7778..8161
                     /gene="queD"
                     /locus_tag="VGB76_10930"
     CDS             7778..8161
                     /gene="queD"
                     /locus_tag="VGB76_10930"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEY0099451.1"
                     /translation="MSGMFEVMIERNFSSAHQLRGYKGKCENLHGHNYKIEIYARGRE
                     LDHIGLLVDFGDLKTAADEIVQYLDHRNINELPPFDAELNPSAENLARYILEHVAARV
                     GDERVKVYKVRCYETPTSVATYQVD"
BASE COUNT         1600 a         2461 c         2626 g         1574 t
ORIGIN      
        1 cgccgagcag gtcgcgtatc tcttcggggt gcgcggggcg cacggcgatt gcgccgagcg
       61 tatcggagag tttcgtctcg tgcagttgat gatcgccgcg aagcagcgcg agcgcgggga
      121 agttttcgcc cgtttcgttc gtggcgatgt agacgagcgt tttaacctgg cggtctgccg
      181 ccgcgccgcc ggggaaggtg atcaaatctt caatcgtgcg gacgccgggc gtgggaaact
      241 cttcgggcgc gccggtcgcc gccgccgggt ctgagatggc tgcgatgcgg gatgtcgctt
      301 tctctaaatt cgccgcgtag ccgcacgctt cacaggtggc gattaagtct tcgcccgcgt
      361 cggacttgac catgaactcg ttggaggcag tgccgcccat cgcgccggag gaggcttcga
      421 cgattgagaa cttgaggccg cagcgcgtga agattttgat gtacgccgcg cgttgatctt
      481 cgaaagactt gtcgagtccc gcgctgtcaa cgtcgaagga gtaggcgtcc ttcatgatga
      541 actggcggac gcgcagcacg cctgacttcg ggcgcgcttc gtcgcgaaac ttcgtctgta
      601 tctgatacca gacctgcggg agttgcttgt aggagcgcag ttcgtggcgg gcgatgccgg
      661 tgaagatctc ttcgtgcgtc atgccgaggc agaggtctgc gcctttgcga tctttgaggc
      721 gaaacatgtt gtcgcccatc accgtccagc gtccggtttc ttcccagatt tcgcgcgggt
      781 tcagagcggg caggaaaaac tcctggccgc cgatggcgtc catctcctcg cgtaccacgc
      841 gcatgacctt gcgcatcacg agccacgaga gcgggagata ggaatagatg cccgcggcga
      901 gttggcgcac catcccggcg cgcagcagaa ggcggtgcga gacgacttcg gcatcagccg
      961 ggacttcgcg caatgtggga atgaaatagt ttgaccagcg catgaaagtg ttttccagtc
     1021 tccttatcga aaaacgtgcg acgcgaacag aacgggcatt gtcgcgcaag ccgcgcgtgc
     1081 aaagcaaacg cccgcgccgc ctccgccttg ccggaacgcg gcggcgggaa gcacaatgag
     1141 gcgacgacgc cacggacgca gatcgtttac gataggctta aacacatgag cgaaaaactg
     1201 cgagtctctt tcaacagccc gcaatgcggc tggatgtcgt tcgagctgcg cgcgggcgaa
     1261 cagagtttga ttgatgccgt cagctacacg ccctacgatt cattacacga cctgatccgc
     1321 gctctttcca aacttctcgt ggacgacacg gagttgacgg tcaagtgggc gtataacccg
     1381 gacgaactgg actttaattt tagagcgagc ggcgacgagg ccgagcttga ggttaagtgg
     1441 tacaaaaatc acctccgggt cgcgggcacg ggcgagcgca ggttcttctt tcaaggctca
     1501 aggctcgacc tgtgccatcc cttctggaag gctctgagag acatccagag cgatacggaa
     1561 gtggacgaat tcacacgcaa ctggaggcgt gaatttcctg aagcagagat gaaacggtta
     1621 accgaagaga tcaaggcgtt caagttaaag taggcgcagg gcgcgaaact gtccgcccct
     1681 tgaaggttgt ttgcgccgcc gcgaaagcgt gatgtaacat ccgttgcgcc ggagagacgc
     1741 tttcccacac agacacaaat attcgaggag aagattcatg accgaccgcc gccacaacgc
     1801 gccgctcgcc gaagttgatc ctttaatcaa cgatgccatc aaccatgaag tcgcgcgtca
     1861 ggccgagggg ctggagttga tcgcgtcgga gaatttcgtg agcgaggccg tgctcgaagc
     1921 gatggggtcg gtctttacga acaagtacgc cgaagggtat ccgaagaagc gttactacgg
     1981 cggctgcgag tttgccgacg tggtggagca agcggcgatt gaccgcgcga aagagttgtt
     2041 cggcgcagag cacgccaacg tccagccgca ttcgggcgcg caggccaaca tgtccgtcta
     2101 tctcgccgcc atccagtatg gcgacacgat cctcggcatg aacctctcgc acggcggcca
     2161 cctcacgcac ggccatccga tgaacttctc cggcatcagc tacaaggtgg ccgactacgg
     2221 cgtctcgccc gaaaccgagc agatcgatta cgacgacctc gcgcgtcgcg ccgaagagca
     2281 ccgcccgaag atgatcgtct gcggcgcgtc ggcctacccg cgcacgattg atttcgcgcg
     2341 cattggcgag atcgcgcgtt cggtcggcgc gcgcgtgatg gccgacatcg cgcacatcgc
     2401 cgggctggtg gcggcgggtc ttcatccttc acccgtgccg cacgcagact acgttacgac
     2461 gacgacgcac aaaactctgc gcgggccgcg cggcggactc atcctgtgcc gcgaagagca
     2521 cgcggcggag gtgaataaaa aactgtttcc cggcgtgcag ggtggtccgc tcgtgcacat
     2581 catcgcggcc aaggccgtcg ccttcggcga ggctctcggc gcggatttca gagagtacca
     2641 gcggcaggtt gtgctgaacg cgagcgcgct cgcggaggaa ctcaaggcgc agggcttccg
     2701 gctcgtctcc ggcggcacgg acaatcatct ggtgctcgtc gatgtcttca tgggaggcgc
     2761 agggatcacc ggcaaggtgg cggagaaggc tctggaagcg gccaacatca cagtcaacaa
     2821 aaacacgatc cccttcgaca cgaacaaacc tttcgtcacc tccggcgtac gcctcggcac
     2881 gcccgcgctc accacgcgcg gcatgggtga aggcgagatg aaaaccgtcg cgcgtctcat
     2941 cgcggaaatt ctacacgccc cggaatccga agaggtgcgc caaaaagtca ggcgcgaagt
     3001 ggcggaactc gccgcacgct tcccgctcta cccgaaccgc ttgcgacaca cgacgccgcc
     3061 cgaagtcgaa gcgatgggcg cggactgagc ttacgtgtgc gcgcgacggc gcgacaattt
     3121 attcgaacgt ataaacgaag atcaaaactc gactgcgaca gttgaaagag cctgagagac
     3181 ttctggcggc gttgcggttc tgctgctacg cggctgccgt gctcgtgcct ttctacctgt
     3241 tgattcgcgc gaactccgcc tggacgccgc accctcgatt ctttcccgac gatgccgtct
     3301 atgcgcggat gtgctggcct tccggcttca ttgtctactg gcacgatttc gggttttatc
     3361 gcctgctcgc ggtgcccgcc tacgcgctct cgaatcgcgc cgtgatgcgt gcttcgctct
     3421 cgcccgtccc gttcacttta atcgcatcgg ccttcgcgac cctcgtgttc gtgtggactt
     3481 gcgtatcggt cggactgtcg cggcggcgcg gcgtgttgct cgcgggcgtg ctgctctctt
     3541 cgccgcttct gcttgagacg ctcaacttct ggtcgggcac gttcaactac gcgctcgtac
     3601 tgctgctcct tgcggcgcaa ctgcgcgcgt gcgtgtgggc gcggcgtcgc ttcgtcgggc
     3661 gaccgtcgcg gggttacctc gtcaccgcct gcggcgcgct catcgcactg ctgacctacg
     3721 agatcgctct gccgttcatc ctcgccacca gcgcgttcta cacgcggcgc gggcgtgcgc
     3781 gcgaactgct gtcggtgtgc atcgcgtgtg ccgcactcct cgcgtttgtg ggcgcgctcg
     3841 tgtgggcggg tgtctactgg ccgctgcgct tcagattgat ggcggagcaa ctggcctccg
     3901 ccgtctccgg cacgcccgcc ccgaatctgc acgcgccgtc tccggctacg gcgacgccgc
     3961 tcagcacgct cggacgcgcg cagatgtacg cctcgctctt gttcagcttc gtgcgcacgg
     4021 gcttgctctc tccttcgttt tggggactcg caggcgcgct ggcgggcttg ggttttcttg
     4081 cgcgcacgcc gacggagaaa gcgagccacc cggcggcagc aagagagacg gaagcgcaac
     4141 cgccggacga tgcggaggca caactgccgg acgataagga agggcgactg ccggatgata
     4201 aggaagggcg actgccgcga gatttggtcg cgctcggttt cgcattctcg ttcggcgcgt
     4261 gcgtcgtcta tcttttggtg acgagatcgc tgaatgcgcg ttacgtcgcg tttttgttaa
     4321 tctacggagc ggcgacgctg gcgtggacga aatggcgcgc ggcctcgttc gcgctcgcct
     4381 cgctgctgct cttgcaggcg gcggtggcgg cgagtttgcc ggttcacatg cgcgatgtag
     4441 agttagcggc gaagggggaa attaacgccg cgcggacggg caggggcgac ctgatctccg
     4501 ttgacgggcg actcacgcgc ggctcttggg gaaagcgttt ttttgcgccg gagccgattg
     4561 acctgttgaa acagccgctc aattcgcgcg cttgccgcta tgccgcgccg tgtgaagaat
     4621 gtcgatgagc gtggtgagag cgcgcgggag attttcgcgc gcttcgcggc atctaaaatc
     4681 gagcgttcgg aaagtgtgag ttggaaacgt tggcgtgagg tgaatgatct ctctatgaaa
     4741 acaagtccga aattttgccg acacgcgctc gcgcttctct tcgccgtgac gatagggaca
     4801 gcgacagcgc aggcgcagaa gcaagcggaa gagaaaatta ttttgccgcc ttcgcctgct
     4861 cctccaatcg cgcaaccgtt gccgacgccg cttcaggcca agcccgtcgc ggataaactc
     4921 gctccggcgg gatggatgcg ttacgaggtc ggagaaccgc tggtcttcag tctgctcctg
     4981 cccacgcaac cgatggggat ggtagaccgt gtgaacgtcg ctcccggcgt ggagggtatc
     5041 tcgcggatct acctgagcgt cgccgacaca ggggtctacg gcgtgtcttt catggacgcg
     5101 ctgcccgccg agcttctgac cgagccacgg aggcgaacgt ttttcgaggg cttcagtcaa
     5161 gagtttgccg aaggctttca ggcacggatg aaggagcgcg ggatggaggt tcgcctcacc
     5221 atgttggagc agcgcgcggc gtcggcgagc gggatgacgg ggtacgagca ggatttttcc
     5281 tacggtcagg tgatggggcg cgtgcgggtg gtcttcggcg cgggaagcgc atatgctgtg
     5341 atggcgatct ggaacggcct ctcgttaaac agcgaacgca gcgcgttctt cgagtcttta
     5401 aaggtcagcg cgaaacgcta acggacgccg ctccacgcgt ctctcgcacg cggataaaaa
     5461 ttatggaaga gattttcggg cgcaacggtt tgatctcgcg ctcgcatccg gattacgaat
     5521 ttcgccccgg ccagatacag atggccgagg ccgtgctgcg cgcgttcgag gaacgcaggc
     5581 acctgctcgt cgaagcgggc acgggtacgg gaaagacgct cgcctatctg gtccccgcga
     5641 ttgcggcggc cacggcgcgc ggagagcgcg tcatcatctc gacgggcacg aagaacctgc
     5701 aagagcagtt gatggaaaaa gatattccgt ttctgcaacg cgtgctgccg aagaagttca
     5761 ccgccgccta catgaagggg cgcaacaact acgcttgcct ccaacgcatc aagcgcgcag
     5821 agtctgcgcc catcctcgac ggccttgatg atatagacca ctttgaagag gtgcgccggt
     5881 gggcgcgcga ttcgcagacg ggcgaccgcg ccgaactcgt gcagcttccc gaaaacctct
     5941 ccttctggcg gcacatcgac gcgcgctcgg aaatctgcat ggggcaaaag tgtcctgact
     6001 acgatccctg cttcatcacg cgcatgcgcc agcgcgcgca ggatgcggac atcgtgatcg
     6061 tcaatcatca tctcttcttc gcagacctcg cgctgcgcgg cggcgagtac gggcaggtct
     6121 tgccggatta ctcggcggtc atcttcgacg aagcccactc gattgaagac gtggcggcgg
     6181 aatatttcgg ccatcaggtg tcgagctatc agatcgaaga cctgctgcgc gacctgcaaa
     6241 ttcttcccgt cacggacgtt gatgcgaaca aggaactgac gcgcgcgggc gggcgcgtgg
     6301 cgcgtttcag cgaacgcttc tggcagggct ttcaggaacg cggcgaagag gggcgcgcac
     6361 cgatcctgcc gggcacgttc gcgcgcaaga acccgcgcgg cgagatcgaa gcgaccgcgc
     6421 tcggcgattc atacctgagc ctcgacggcg cgctcaaccg tatggagacg acgctcgacg
     6481 cgctgcgcga gcagccgccc gaagtggaga acctgattcg ccgcattcgc gaagtgcgtt
     6541 tcgggctgga gttcatcgtc gcgggtgacg acaagcgtta cgtctactgg atggagcggc
     6601 gcgggcgcgg catgttcctg cgcgcctccc cgattgatgt ctccactctg ctgcaagaca
     6661 aactcttcga gcgcgtcgag acggtcgtga tgacttcggc cacgctggcg agcgcgggca
     6721 gcttcacgtt tgcgcgcgaa cgactcggcc tcaacgatga gaagacggac gaactcatcg
     6781 cgccttcgag ttacgactat caaaaccagt cggtcttgta tctcccgccg cgtatgcccg
     6841 accctcgttc gccgcagtgg gcggaagcgg cggcggcaga agtcctgcgc atactggaag
     6901 tgaccgacgg gcgagccttc gtgctctcca cgagtttgag cgggatgcgc gcgctctacg
     6961 aacgcgtcgc gccgcaagtt gattttccct gtttcatgca gggcacggcg gcgaagtctg
     7021 cgctgctcga taagtttcgc acgacgccga acgcagtgct cttcgccact tcgagtttct
     7081 ggcagggtgt ggacgtgcgc ggcgacgcgc tctcgtgcgt catcattgat aagctgccgt
     7141 ttgccgtgcc gtccgacccg attgtggccg cgcgtcaacg cttcatcgaa gacgcgggcg
     7201 ggtcgagttt ctacgagtac agcgtgccgc aggcgatcat cagtttgaag cagggcttgg
     7261 gtcgtttgat tcgcagcgcg acggacaggg gcgtgctctc cgtgctcgac ccgcgcctgc
     7321 ggacgcaacc ttacgggcga cagttcatca agagcctccc gccctgccgc gtcacctccg
     7381 acctgaaaga ggtggcgcgc gtgtttgttg aaagcgcggc ggctgttaga tgatagcgtc
     7441 cgtcgaaaac aagggggcga gttctaacca aaactttcga cctctggagg cgcgcatgag
     7501 caaagacgca agcgaagata agacgatcta caaagtggtg gtaaaccggg atcagtattc
     7561 catctggcct gctcaccgtg agaacgcgcg gggctggacg gatgccggtc aaagcggaac
     7621 taaacaacaa tgtctcacct acattgaaaa ggtttcgaag gccgcccgac cgcctgctga
     7681 gaagaagcag cggtaagaga tgcggctagt ccatccgtca gtctcaagcg tgagggcggg
     7741 cggcatcagc cgggcagaga gggaagcgaa gcagaagatg agcggaatgt ttgaagtgat
     7801 gatcgagcgc aacttttcga gcgcgcacca actgcgcggc tacaaaggga agtgcgagaa
     7861 cctgcacggg cacaactaca agatcgaaat ctacgcgcgc gggcgcgaac tagaccacat
     7921 cggactcctc gtagacttcg gcgacctgaa gacggcagcc gacgagatcg tacagtatct
     7981 cgatcatcgc aacatcaacg aactcccgcc cttcgacgcc gaactcaacc cctctgcgga
     8041 aaacctcgcg cgctacatct tggagcacgt cgccgcgcgc gtcggcgacg aacgcgtcaa
     8101 agtctataaa gttcgctgct acgaaacgcc gacgagcgtg gcgacgtatc aggtggatta
     8161 acccactcac gaagaagcgt gcgcgcttaa gacaaaagcc agtgagcttt tgttatacgc
     8221 aacccggctt ccgtcaacag cacacacact ttcattataa g
//
LOCUS       DASWNS010000311         3700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_297546, whole genome shotgun sequence.
ACCESSION   DASWNS010000311 DASWNS010000000
VERSION     DASWNS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297546"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="VGB76_10935"
     CDS             complement(<1..1591)
                     /locus_tag="VGB76_10935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY0099452.1"
                     /translation="MDDRVLPDDKVSPVLRDRIRASRPNDVILSVILQLREKPKGELS
                     ALLNRNGVHVNATYESFNSMAVELPAGVVEELTLFDEVKLITEDQEVNFLGFVESVTG
                     AWEMRERSGNSGLKGKDMNIAVLDSGVDRDHHGVNDIAYQKDFTGENRTDDPYGHGTH
                     VASLASASDHIAHGAYTGLAPEAKIINLRVLNSQGIGSTANVLKALQWILAPTDPNKP
                     LGEKNHQKYKIRVVNMSIGSPAINSYKVDPLCLAVRKLVDTGIVVVAAAGNDGTTMDG
                     QKFYGRIHSPGNEPSAITVGASNTHDTESRHDDVMTTYSSRGPTRSYQTVNGARVYDH
                     IVKPDLVAPGNKLIAAEALDNAIVRNYPSLDVTPASNPKHKVMLMSGTSMATPIVAGA
                     AAVLLQANPKLTPNMIKMILQYTAQPLKGFNTYEQGAGQLNLDGAVQLAQLIRTDLTN
                     LTKLGTPLLVAGSPLPSPYTTLSGYKFEWAQGVLQNQGYITGTNLITKYQRLYNLGAA
                     FGQGIMISDGIMISDGVKLSDG"
     gene            complement(2165..3067)
                     /locus_tag="VGB76_10940"
     CDS             complement(2165..3067)
                     /locus_tag="VGB76_10940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0099453.1"
                     /translation="MSLTLLRQEVMAEAPFLPEANVTVTINKSTDAEREEVLAFLSEN
                     PVHTVVMASLIRDHGIQSEYNRGTFWVCRNPKGRLEGVALIGHVTLVEARTVRAAREF
                     AFVAQRSVRKHLILGERQKIEAFWAFYAGEGQPVRRACRERLFELRRALEVDGEVRGL
                     RRATTEDIDLIAPVHAAMAEEESGINPLNIDPGGFIARCRRRIEAGRVWVNIKDGRLL
                     FKADVQAETPDVVYLEGVWVAPSERDTGFGRRCLTHLCTDLLTKTKSVCLLVNEENVR
                     AHAFYRACGFRCEEVFDTIFLQRN"
     gene            complement(3089..>3700)
                     /locus_tag="VGB76_10945"
     CDS             complement(3089..>3700)
                     /locus_tag="VGB76_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY0099454.1"
                     /translation="GDGVLISDGVLISDGVLISDGVLVSDSIVTSSGVLMGDGTVFCG
                     TGVLIGDGVLMGDNMVFGDGVLIGDGVLIGDGVIIGDVSNAANSFMINGDRTASMPVV
                     PDISVAAPNAPSTLVVATASTSQLNLSWKDMSANEDGFQIERCAGATCTNFAQVAQVK
                     ANLPTFASAGLNKATTYRFRVRAVNLGGYSNYTPIVNGTTKSR"
BASE COUNT          679 a         1056 c         1157 g          808 t
ORIGIN      
        1 ttccgtcgga gagcttaact ccgtcggaga tcataatccc gtccgagatc atgatgccct
       61 gaccgaaggc cgcgccgagg ttgtaaagcc gctggtattt cgtgatcagg ttggttccgg
      121 taatgtagcc ctgattttgc agcacgccct gcgcccactc gaatttgtat ccgctaagag
      181 tggtgtaagg cgaagggagc gggcttccgg cgacgaggag tggcgtgccg agtttggtca
      241 ggttggtgag gtctgtgcgg atgagttgcg ccagttgcac tgcgccgtcg aggttgagct
      301 ggcctgcgcc ctgctcgtag gtgttgaagc ccttgagcgg ctgcgccgtg tactggagga
      361 tcatcttgat catgttcggc gtgagcttcg gattcgcctg aagcagcacg gctgccgcgc
      421 ccgccactat gggcgtcgcc atcgaagttc cgctcatcag catgaccttg tgtttgggat
      481 tgctcgcggg cgtcacgtcg agcgaagggt agtttctgac gatggcgtta tccagtgctt
      541 cggcggcgat caatttgttg ccgggggcga cgaggtcggg tttgacgatg tggtcataga
      601 ccctcgcacc gttgacggtc tggtagcttc tggtcgggcc acgcgaactg taagtcgtca
      661 tcacgtcgtc gtgacgagat tcggtgtcgt gcgtgttcga cgcgccgacc gtgatggccg
      721 aaggttcatt gccggggcta tggatacgcc cgtaaaactt ttgcccgtcc atagtcgtcc
      781 cgtcgttacc ggcggcggcg acgacgacga tgcccgtatc aaccagcttg cgcaccgcca
      841 ggcagagcgg gtcaaccttg tacgaattga tcgccggtga gccgatgctc atattaacga
      901 cgcgaatctt atacttctgg tggttcttct cgccgagcgg cttgttaggg tctgtcggag
      961 ccagtatcca ttgcaacgct ttcaggacgt tagcggtaga gccgatcccc tgcgagttga
     1021 gaacgcgtag gttgatgatc ttcgcttcgg gcgcgaggcc ggtgtacgcg ccgtgggcga
     1081 tgtggtcgga cgcggaggcg agggatgcga cgtgcgtgcc gtggccgtag gggtcgtcgg
     1141 ttcggttctc gcccgtgaaa tctttctggt aggcgatgtc gttgacgccg tggtgatctc
     1201 tatcgacgcc ggagtcgagc acggcgatgt tcatgtcctt gcctttgagg ccgctgttgc
     1261 cgctgcgctc gcgcatctcc cacgcgcccg tcacgctctc aacgaatccg aggaagttca
     1321 cctcctgatc ttccgtgatg agcttaactt cgtcgaacaa ggtgagttct tcaaccaccc
     1381 ctgcggggag ttcgaccgcc atcgagttga agctctcgta agtggcgttg acatgaacgc
     1441 cgttgcgatt gagcagggcg ctgagttcgc ccttcggctt ttctctaagc tggagaatga
     1501 cggagaggat tacgtcgttc gggcgcgaag cccggatgcg gtcacgcagc acaggcgaga
     1561 ctttatcgtc gggcagcacc cggtcgtcca cgggtctttc ttccctgacc tgtcttttac
     1621 cgggctgtct tttggtctgc tgcgcgccga ccggcaagag cgagtgggcg ccggaaataa
     1681 gcgcgacgag cagcagcgct gttaatctta tgcatttccg ggatcgtcgt gaagaattca
     1741 tgttcctgtc cttcgggaac tcccactacc ttgctcctgt cgaggagttt aggctggtaa
     1801 gtgcgtgccc gctcccgttt tcacagagag gaggctcttg tcggtgactt gatagctaaa
     1861 gagcgtgccc ggcgcaggcc gggctgccgg ttaataagcg atagtcgtgc cataagggca
     1921 aagccggtga tcgccgtaaa ctgcggatta aacgggcgac gtgcgagaac tacaggcatt
     1981 atcccggaac cagaatgacc gattcattca aaagtaatgg cagcgactgt cacaatgaac
     2041 gacgtgcggt gaggtgctat agaggtaaaa cgatgaagta gttatgatga cccgttcata
     2101 aaagaagcag agcagacccg gcagcccggt ctgctctgct tcatgacaat attacgaatg
     2161 gagttcagtt gcgctgtaga aagatggtgt cgaacacttc ctcgcaacgg aagccgcacg
     2221 cgcggtagaa ggcatgcgcc cgcacgttct cctcgttcac gagcaggcag acgctcttcg
     2281 ttttcgtaag caggtcagtg cacaagtgcg tcaggcaacg cctgccgaag cccgtatcgc
     2341 gctccgaggg agctacccac acgccttcga ggtagaccac gtccggcgtc tcagcctgca
     2401 cgtcggcctt gaagagcaac cttccgtcct tgatgttcac ccacacgcgc cccgcttcga
     2461 tgcgacgacg gcacctcgca atgaaccctc cggggtctat gttgagaggg ttgatcccgc
     2521 tctcctcctc ggccatcgcg gcgtgcaccg gcgcgataag gtcaatgtct tccgtggtag
     2581 cccggcgcag ccccctgacc tctccgtcta cttctaacgc gcggcgcagt tcgaagagtc
     2641 gctcgcggca ggcgcgcctc accggttgcc cctcaccggc gtaaaacgcc caaaaggctt
     2701 cgatcttttg gcgctcgccc aagatcaggt gcttgcgcac actacgctgc gcgacgaagg
     2761 cgaactcgcg cgcggctcgg acggtgcgcg cctcgacgag cgtgacgtgg ccgatgaggg
     2821 cgacgccttc gaggcggcct ttcgggttgc ggcagaccca gaaggtgccg cgattatatt
     2881 cgctttgaat cccgtggtcg cggattaagc tcgccatgac gacggtatgt acggggtttt
     2941 cggagaggaa ggcgaggact tcttcgcgct ccgcgtcggt ggatttattg attgtaaccg
     3001 tgacgttcgc ctccgggagg aagggagcct cggccatcac ttcttgtcga agtaaggtca
     3061 gtgacatggc tgttgttcct tgcccggtct aacggctctt ggtcgtaccg ttgacaatcg
     3121 gtgtgtagtt cgaatagccc cccaaattaa ccgccctcac gcggaagcgg taagtggtgg
     3181 ccttgttcag acccgcgctg gcgaaggtgg ggaggtttgc cttgacctga gcgacctgcg
     3241 cgaagttagt gcaggttgcg cccgcgcacc gctcgatctg gaagccgtcc tcgttggccg
     3301 acatgtcttt ccatgagagg ttcaattgcg aagtcgaagc ggttgccacc acgagcgtcg
     3361 agggcgcgtt cggagcggcc acactgatgt ccggaaccac cggcatggag gctgtccggt
     3421 cgccgttgat catgaacgag ttggcggcgt tggagacatc accgatgatc acgccgtcgc
     3481 ctataagcac gccgtcgccg atgagtacac catcgccgaa caccatgttg tcgcccatga
     3541 gtacgccgtc gccgatgagc accccggtgc cgcaaaagac cgtgccgtcg cccatcagca
     3601 cgccgctgct ggtgacgatg ctgtcgctga caaggacgcc gtccgagata agcacgccgt
     3661 cactgataag tacgccgtcc gagataagca cgccgtcgcc
//
LOCUS       DASWNS010000312         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_141415, whole genome shotgun sequence.
ACCESSION   DASWNS010000312 DASWNS010000000
VERSION     DASWNS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141415"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1860
                     /locus_tag="VGB76_10950"
     CDS             49..1860
                     /locus_tag="VGB76_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0099455.1"
                     /translation="MSNHSARSSANSLAARLERRSRQTILSMLFALVVAALFAPFSAA
                     TVRAQSPVWTHKPAQEIKWYRLVEAGTLLVGTDASLYMLNPESGQTLWQREDLRGVAE
                     YQTQEIAGTPLLLVSQNSGRIQGKTKLSALDALTGQSVWETEKLKGATVQVSPVYDKD
                     MVLLLTTESASKAKDKLDITALKLSTGATLWESEFTSNVDLYGMERGSKYFPKFDLSG
                     AQPPVFDGDSVYFTYAGLHRYNLSDGKLVWKVDYDVTEGKIKQGNAQAVIDGDTIYTS
                     AKGQVRALDKANGALRWTSKDFGGAVAEMQLRAGVLYGRLGGTFYDFGKREYVVKKPL
                     GVVALDPKAGTANWFYDKADDSITNMAFLPEQNQVLIADSKNLIALDTTATGKVKEAY
                     KVKLEFKFNLGTAATVAKIAKFGFGGLSALGSKGADTTDEPVSISRRENGTVVVRGKQ
                     HLLAFDPRTKQIPWSTKYAAPGVPGWQKIAMTAITIASAAMSNSQEAAYASRGDWRSA
                     GDSNRNFINAMSSYEQFMSKRHTATKAGSLYVYVLTDVKQDKEKGAGVVGVNMETGQG
                     DRQIMFKDKEPDYEVDEASGRVFNLRNSKELNAYILK"
BASE COUNT          438 a          582 c          562 g          306 t
ORIGIN      
        1 ttaggccaac agacattcaa ccaactcagg agatagagac gttcccccat gtcaaatcac
       61 tccgcccgct catcggccaa ctcactcgcc gcgcgtctcg aacgccgctc tcgtcaaaca
      121 atcctgtcaa tgcttttcgc gctcgtcgtc gcggcgttgt tcgcgccctt ctccgccgct
      181 acggtgcgcg cgcagtcgcc cgtgtggacg cacaagcccg cgcaggagat caagtggtac
      241 cggctggtcg aagcgggcac gctcctcgtc ggcacggacg cgagcctcta catgctcaat
      301 ccggagagcg ggcagacgct gtggcagcgc gaagacctgc gcggcgtcgc agagtatcaa
      361 acgcaggaga tcgcgggcac gccgctcctc ctcgtcagtc aaaattcggg acgcattcaa
      421 ggcaagacga aactctccgc gctcgatgcc ctgaccgggc agagcgtgtg ggagacggag
      481 aaactcaaag gcgcgaccgt gcaagtctcg cccgtctacg ataaggacat ggtgctcctg
      541 ttgacgaccg agagcgcgtc caaagcgaag gataagctcg acatcaccgc gctcaaactt
      601 tccacgggcg cgacgctgtg ggaatcggag ttcacttcaa acgtcgatct ctacggcatg
      661 gagcgcggca gtaagtactt tccgaagttc gacctctccg gcgcgcagcc gcccgtcttc
      721 gacggcgact cggtctattt cacttacgcc ggacttcatc gctacaacct ctccgacggc
      781 aaactcgtct ggaaagttga ctacgatgtg accgaaggca agatcaaaca gggcaacgcg
      841 caggccgtga ttgacggcga cacgatctat acgtccgcga aaggacaggt gcgcgcgctc
      901 gacaaagcga acggcgcgct gcgttggacg agcaaagatt tcggcggcgc ggtggccgag
      961 atgcagttgc gtgcgggcgt gctctacggg cggctcggcg gcacgttcta cgatttcggc
     1021 aaacgcgagt acgtcgtcaa gaaaccgctc ggcgtcgtcg cgctcgaccc gaaggcgggc
     1081 acggccaact ggttctacga taaggccgac gacagcatca ccaacatggc cttcctcccc
     1141 gaacagaatc aggtgctcat cgccgacagc aaaaatctga tcgcgcttga tacgacggcc
     1201 acgggcaaag tgaaagaagc ctacaaggtc aaactcgaat tcaagttcaa tctcggcacg
     1261 gcggcgacgg tggcgaaaat cgccaagttc ggcttcggcg gactctccgc gctcggctcc
     1321 aaaggcgcgg acacgacgga cgaacctgtc tccatcagcc gacgcgaaaa cggcacggtc
     1381 gtcgtgcgcg gcaaacaaca cctgctcgcc ttcgacccgc gcacgaaaca gattccctgg
     1441 tctacgaagt atgccgcgcc gggcgtcccc ggctggcaga agatcgccat gacggccatc
     1501 acgattgcga gcgcggcgat gtcgaactcg caggaggcgg cctacgcctc gcgcggcgac
     1561 tggcgaagcg cgggcgattc aaatcgcaac ttcatcaacg ccatgtcctc ttatgagcag
     1621 ttcatgtcga agcgtcacac ggcgacgaag gctggcagcc tctacgtcta tgtgctgacg
     1681 gacgtgaagc aggacaagga gaagggcgcg ggcgtcgtcg gcgtcaacat ggagacggga
     1741 cagggcgacc gccagatcat gttcaaagac aaagagccgg actacgaagt tgacgaagca
     1801 tcggggcgcg tcttcaactt gagaaacagc aaggaactga acgcctacat cttaaaatag
     1861 aagaaaacga tgaagccgga acgcggaa
//
LOCUS       DASWNS010000313         6116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_90223, whole genome shotgun sequence.
ACCESSION   DASWNS010000313 DASWNS010000000
VERSION     DASWNS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90223"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VGB76_10955"
     CDS             complement(<1..607)
                     /locus_tag="VGB76_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY0099456.1"
                     /translation="MSKAEDPLTIKEASVWASEYLGKNVTPSNITYLINYGRIPKLDN
                     NDSTRVSKDDLIEYYSAYKNSREHSWKNQLGEDLNWALSFDQYKESETTKHVHRLHPY
                     KGKFIPQLVEYFLDDHTDNFKKEVFFKKGDIVLDPFCGSGTTLVQSNELGINAIGIDI
                     SAFNALISNVKVQKVDLENLKLESDKITIALRELVRDAKHLA"
     gene            786..2240
                     /locus_tag="VGB76_10960"
     CDS             786..2240
                     /locus_tag="VGB76_10960"
                     /inference="COORDINATES: protein motif:HMM:NF016021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099457.1"
                     /translation="MGTLNIDRYHATMGDASYKDVTRLHDKSLSEAEATFYITQRKLA
                     FAPCLGHERLVRLMGESGIDRPRIRFLEQDRGGLERFAKAVEDLSFIGAVRAVRPATI
                     MFAGEPFADITGAFGLTQAQEIKFEHAFDLPMTTASLALQFRMAAGEHRWLSDFSLRR
                     NGDIERAVEVATYAFIGGFNDTSNMEAAHRLDIPAVGTSAHYWQQAYIEYIEKPEIEP
                     RTGRPKHFEQVAFERWLDANPLGTTLLLDTIDVYTGAAHAAMAATSTPERRHAFKGFR
                     VDSGDLAKLGAWCLRFFEANGLHGLMPVLTGDLDVETVRRIVSEFPQAVGFGVGTKLS
                     GEVRRIAGVIFKQCLIEGRPTLKGSNTPEKSTLPGRLQVFRGVDRENYYVGDIIGLDE
                     EEVEIPGAVAVERLLIPFWERGRYESIPSITKQKAFVEEQRARFRDLENYPHALSDKL
                     RALRDELTRRMRADDSNWQAVLQLPEELKSKDEG"
     gene            2324..3646
                     /locus_tag="VGB76_10965"
     CDS             2324..3646
                     /locus_tag="VGB76_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0099458.1"
                     /translation="MPEQTSPPLNWSKRRLRLFKFALLLGALSFGLLVAEIALRISGY
                     TYPLFYTTDVVRGYALKPGMSGWYRKEGAAYIRINSDGLRDREHDTAKPADTVRIAVL
                     GDSYAEALQVPLEDAFWVVMEERLRSCPAFAGQRIEVINFGVSGYGTAQELLTLRERV
                     WSYAPDIVLLAMTTNNDITDNLRALKRTEEIPYFVYRDGYLVLDDSFRQSRTFQLRHS
                     ALNRAGRWIRDQLRVIQAFHQGHYALKNYLASRRTHAAPAATPPPPAPAAQPDGQTPA
                     PATNAPATNAPAQRDAVVISDELGIDNLVYREPADANWQEAWRVTEGLLGFMHNEVRD
                     HGALFVVATLSNGVQVHPQPAAREAFLRRVGATDIFYPDARIKSLGERAGFPVLTLAP
                     ELQQYAERQQTFLHGFGRDLGNGHWNSSGHRLAGELLAQKLCATMENN"
     gene            3706..5100
                     /locus_tag="VGB76_10970"
     CDS             3706..5100
                     /locus_tag="VGB76_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099459.1"
                     /translation="MKTSLTTESTRDIIARLQQANTDFARDYPGETGRRQPVHTVYGG
                     AHLFKSDTPRRLGTLALRSLEQFAPDPHAFARALGLHNAEGAEATHDKGVDAVLVSII
                     YERVQEKLRREPVEDFRIDFEDGYGNRPDAEEDGHAEACAREVASGLKSGTLSPFIGI
                     RIKPFTEELRARSIRTLDIFVSTLVSEANGALPPNFVVTLPKITIPEQTAALASLFEQ
                     LEPQLSLPTGALKFEMMIETTQSIINHSGAINLPLLLAAARGRCIAAHFGTYDYTASC
                     NITAAHQHMTHPSCDFAKHMMQVSFAGTGVWLSDGATNIMPVAPHRAQQGGDALTPEQ
                     EEENRRTVQRAWRIHYEHVRHSLTGGFYQGWDLHPAQLPTRYAAVYAFFLQSLDAASE
                     RLRNFVEKAAKATLVGDVFDDAATGQGLLNYFLRAINCGAITEAEALERSSLTLDELR
                     SGSFVKILNRRQNL"
     gene            5322..5807
                     /locus_tag="VGB76_10975"
     CDS             5322..5807
                     /locus_tag="VGB76_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099460.1"
                     /translation="MRSNGKGFLTKVTMLAMGLFLMLPLASVAAAQDRGWYGQDRRDD
                     RRDRRRDRREERREERREERQERREERRARRGNSYDGYPNYGGTFQLRQTALNAGYNE
                     GIKEGRKDRSRNERYDFSDESVYRSGTKDYSSRLGDRSLYQRYLRMAFENGYNDGYSG
                     Y"
     gene            complement(5937..>6116)
                     /locus_tag="VGB76_10980"
     CDS             complement(5937..>6116)
                     /locus_tag="VGB76_10980"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY0099461.1"
                     /translation="LAAQAKLRSKQNTYMVVPVVFTMISNHFPTATYGSRYDWIILSV
                     LILVGWLAAKFIRRA"
BASE COUNT         1289 a         1915 c         1712 g         1200 t
ORIGIN      
        1 aagccagatg ttttgcatcc cggaccagct ctcttagtgc aatagtgatt ttatcacttt
       61 caagttttag gttttccaga tcaacttttt ggacttttac gttcgagatc aaagcattga
      121 aagcagaaat atcaatacct atcgcattaa tacctaattc attggattga accaaagttg
      181 tgccgcttcc gcaaaaaggg tcgagcacaa tatcgccttt cttgaagaaa acctctttct
      241 tgaaattgtc cgtgtggtcg tcaagaaagt attcgactaa ttgtggaata aattttcctt
      301 tatatggatg aagccggtga acgtgtttgg tcgtctccga ttctttgtac tgatcgaatg
      361 acaaagccca gtttaagtct tcacccagtt gatttttcca ggaatgttct ctggagtttt
      421 tataagcgct gtaatattca atcaggtcgt cttttgagac acgagttgag tcgttgttat
      481 cgagcttagg aatacgacca tagttgatga gataagtgat gtttgaaggc gtgacatttt
      541 tgcctagata ttcggatgcc caaacactgg cttctttaat agttagaggg tcttccgctt
      601 ttgacatgtc ttctgaatcg ctttaaatct taaactcttt acagtctact ttcgccgctc
      661 gccgcgatta tagcaaacgc actttgcctg ctccgcgccg acgcttattt tacgggctga
      721 gatattctac aatgcccgtc gaataatatc ttaagagccg tctgttcgtc cctgcacctt
      781 aaattatggg aactctcaat attgatcgtt atcacgcgac gatgggcgat gcgtcgtaca
      841 aggacgtgac gcgtctgcac gataaatcct tgagcgaggc ggaggcgact ttttacatca
      901 cgcagcgcaa actcgctttc gccccgtgtc taggccatga gcggctcgtg cgtctgatgg
      961 gcgagagcgg gatcgaccgc ccgcgcatac gctttttaga acaggacagg ggcggccttg
     1021 agcgtttcgc aaaggccgtg gaagacctga gttttatcgg ggcggtgcgc gccgtcagac
     1081 ccgcgacgat catgtttgcg ggcgagccgt tcgcagacat cacgggcgcg ttcggactga
     1141 cgcaggcgca ggagatcaag tttgagcacg ccttcgatct gccgatgacg accgcctcgc
     1201 tcgcgttgca gtttcgcatg gcggcgggcg agcatcgctg gctctccgat ttttccctgc
     1261 gccgcaacgg cgacatcgag cgcgccgtcg aagttgcgac ctatgccttc atcggcggtt
     1321 tcaacgacac gtcgaacatg gaggcggcgc accgtctgga tattcccgcc gtcggcacct
     1381 ccgcgcacta ctggcagcag gcttacatcg agtatatcga aaagccggag atcgagccgc
     1441 gcaccgggcg acccaagcat ttcgagcagg tcgccttcga gcgttggctc gacgccaacc
     1501 cgctaggcac gacgctcctg ctcgacacca tagatgtcta cacgggcgcg gcgcacgccg
     1561 cgatggccgc cacttccacg cccgaacgcc gccacgcctt caaaggcttc cgcgtggatt
     1621 cgggcgatct ggcgaaactc ggcgcgtggt gtttgcgctt ctttgaagcg aacgggctgc
     1681 acggtttgat gcctgtgctg acgggcgatc tcgatgtgga gactgtgcgg cgcatcgtgt
     1741 cggagtttcc gcaggccgtg gggttcggcg tcggcacgaa gttgtcgggc gaggtgcgac
     1801 gcatcgcggg cgtgatcttc aagcaatgcc tgatcgaagg ccgccccacg ctcaagggtt
     1861 ccaacacgcc ggagaagtct acgctgcccg gacgcttgca ggtctttcgc ggcgtagacc
     1921 gtgagaacta ctacgtcggc gacatcatcg gcctcgacga agaggaggtt gagattcccg
     1981 gcgcggtcgc cgtcgaacgc ctgctcattc ccttctggga gcgaggccgt tacgagtcca
     2041 tcccttccat caccaaacag aaagccttcg tcgaagagca acgcgcacgc ttccgcgacc
     2101 tcgaaaacta cccgcacgct ttgagcgaca aactccgcgc cctgcgcgac gaactgacca
     2161 ggcgcatgcg cgcggacgac tcgaactggc aggccgtcct gcaactgccg gaagaactca
     2221 aaagcaagga tgaaggatga gggatgaggg atgaacaaaa caaagcactc acacgcttga
     2281 atgcttttct gattcatccc tcatccctca tcccttactg attatgccgg aacaaacgtc
     2341 gccgccgctc aactggtcga aaagaagact gcgcctgttc aagttcgcac tcctgctcgg
     2401 cgcgctcagc ttcgggctgc tcgtcgcgga gatcgcgcta cgcatcagcg gttataccta
     2461 tccgctcttt tacactaccg acgtggtgcg cggctacgcg ctcaaaccgg gcatgtccgg
     2521 ctggtatcgc aaggagggcg cggcctacat ccgcatcaac agcgacggcc tgcgcgaccg
     2581 cgagcacgac acagcgaagc ccgccgacac cgtgcgtatc gcagtgctcg gcgactctta
     2641 cgcggaggct ttgcaggtgc cgctcgaaga tgccttctgg gtcgtcatgg aagagcgtct
     2701 gcgaagttgt ccggctttcg ccgggcagcg catcgaagtg atcaacttcg gcgtctccgg
     2761 ctacggcacg gcgcaggagc ttctgacgct gcgcgaacgt gtctggagct acgcgcccga
     2821 catcgtgctc ctcgccatga ccaccaacaa cgacatcacg gacaacctgc gcgccctcaa
     2881 acgcacggaa gagattccct acttcgtcta tcgcgacggc tacctcgtgc tcgacgattc
     2941 cttccgccag tcgcgcacgt tccagttgcg ccactccgct ctgaatcgcg cgggacgctg
     3001 gatcagggat caactgcgcg tcatacaggc ttttcatcaa ggccactacg cgctcaaaaa
     3061 ctatctcgca tcgagaagaa cacacgccgc tcctgccgcc accccgccgc ctcccgcgcc
     3121 cgccgcgcaa cccgacggcc aaacaccagc ccccgcgact aacgcccccg caacgaacgc
     3181 cccggcgcaa cgcgacgccg tcgttatctc cgacgaactc ggcatagaca atctcgtcta
     3241 tcgagaaccg gcggatgcca actggcaaga ggcctggcgc gtgaccgaag ggctgctcgg
     3301 gttcatgcac aatgaagtgc gcgatcacgg cgcgctcttt gtagtcgcca cgctcagcaa
     3361 cggcgtgcag gttcacccgc aacctgctgc gcgagaggct ttcctgcggc gcgtcggcgc
     3421 aaccgacatc ttctacccgg acgcgcgcat caagtcgctc ggcgagcgcg cggggtttcc
     3481 cgtcctcacg ctcgcgccgg aacttcaaca atatgcggag cgacagcaaa ccttcctcca
     3541 cggcttcggg cgcgatctcg gcaacggcca ctggaactca agcggccacc gccttgcggg
     3601 cgaactgctc gcgcaaaaac tctgcgcaac gatggagaac aactgagtgc gacctgcgct
     3661 ccgctatact tatccacgtc aatctccggc tccaggaagg cacacatgaa aacatctctg
     3721 acgaccgaat ccacgcgcga catcatcgcc cgcctgcaac aggccaacac ggactttgcg
     3781 cgcgactacc ccggcgaaac cggacgccgc cagcccgtcc acacggtcta cggcggcgcg
     3841 cacctcttca aatccgacac gccgcgccgt ctcggcacgc ttgccctgcg ctccctcgaa
     3901 caatttgcgc ccgacccgca cgccttcgca cgcgccctcg gcctccacaa tgcggaaggg
     3961 gcggaagcga cgcatgacaa aggcgtggac gccgtgctcg tcagcatcat ctacgaacgc
     4021 gtgcaggaga aactcaggcg cgagccggtc gaagactttc gcatagactt cgaggacggc
     4081 tacggcaatc gtcccgacgc ggaagaagat ggtcacgcgg aagcgtgtgc gcgtgaagtg
     4141 gcgtcggggt tgaagtccgg cacgctctcg cccttcatag gcatccgcat caagcccttc
     4201 acggaagaac tgcgcgcacg cagcatccgc acgctcgaca tcttcgtctc cacgctcgtc
     4261 tccgaagcga acggtgcgtt gccgccgaac ttcgtcgtca cgctcccgaa gatcaccatc
     4321 cccgaacaga ccgccgcgct cgccagtctc ttcgaacagt tggagccgca actctcgctc
     4381 cccaccggcg cgctcaagtt cgagatgatg atcgaaacca cgcagtccat catcaaccac
     4441 agcggcgcga tcaatctgcc gctgctgctc gccgccgcgc gcggtcgctg catcgccgcg
     4501 cacttcggca cctacgacta cacagccagt tgcaacatca ccgccgcgca ccagcacatg
     4561 acgcatcctt cgtgcgactt cgccaagcac atgatgcaag tctctttcgc gggcacgggc
     4621 gtctggctct ccgacggcgc gaccaacatc atgcccgtcg ccccacaccg cgcgcagcag
     4681 ggcggcgacg cgctcacgcc cgaacaggaa gaggagaacc gccgcaccgt ccaacgcgcg
     4741 tggcgcatcc actacgaaca cgtccgccac tcgctcacgg gcggcttcta tcaaggttgg
     4801 gacttgcacc ccgcgcaact ccccacgcgc tacgcagccg tctacgcctt cttcctacag
     4861 agcctcgacg cggcctccga acgcctgcgc aacttcgtcg agaaggccgc taaagccact
     4921 ctcgtcggcg acgtgttcga cgatgccgcc accgggcagg gcttgctcaa ctatttcctg
     4981 cgcgccatca actgcggcgc gatcaccgaa gcggaagcac tggagcgttc cagcctgacg
     5041 ctcgacgaac tgcgctccgg ctccttcgtc aagattctca accgcaggca gaatctataa
     5101 ttttgtatgg cgcagttcgc cccaagccgt gccccgtgcg tatgcacacg atacggaaca
     5161 ccttgacacc taatcgtgcg cgccggaacg tttcgttggt tgaagagcgg ctaaattcca
     5221 gcgtttgcct gtgcaggcgc gtgtggcacg gcggttgtta cttagtccgg cgaggtttcg
     5281 cgatgttgcg aaattccagt tgagagtgga gggtgataaa gatgagaagc aatggtaagg
     5341 gatttttgac taaagtgacg atgctggcga tgggactttt tctgatgctg ccgttggcat
     5401 ccgtcgccgc cgcgcaggac agagggtggt atggtcaaga ccgccgcgat gaccggcgcg
     5461 accgccgcag agatcgacgc gaagagcgac gcgaagagcg acgcgaagag cggcaggaac
     5521 ggcgcgaaga acgcagagcg cgtcgcggca acagctacga tggctacccg aactatggtg
     5581 gcacgtttca gcttcgacag acggccttga acgccggtta caacgaaggc atcaaggaag
     5641 gtcgcaagga tcgttcgcgc aacgagcgtt acgatttcag cgacgagagc gtctatcgca
     5701 gcggcacgaa ggactacagt tcacgcctcg gcgaccgttc cctgtaccag cgataccttc
     5761 gcatggcctt tgagaacggc tacaacgatg gttacagcgg ctactaagaa gcggctgaca
     5821 acattcaaac tcgaacggga gcgggagaca ataacgtctc ccgctcccgt ttactttctg
     5881 cccgtcctgt aactattgag atgtgcggca gtcgtctaaa acctgtcaaa cgagtttcac
     5941 gcgcgccgta tgaacttcgc ggcgagccag ccgacgagga tcaggacgga gagaatgatc
     6001 caatcgtagc ggctgccgta ggtagcggtc gggaagtggt tgctgatcat cgtgaaaacg
     6061 acgggcacga ccatgtaggt gttctgcttg gagcggagtt tggcctgcgc ggcgag
//
LOCUS       DASWNS010000314         5182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_166850, whole genome shotgun sequence.
ACCESSION   DASWNS010000314 DASWNS010000000
VERSION     DASWNS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166850"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VGB76_10985"
     CDS             complement(<1..1458)
                     /locus_tag="VGB76_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEY0099462.1"
                     /translation="MNIRSSLTTTFSALLLCWLTALPLAAQSARRVPTVDDLLALKSV
                     GAPRISPDGKWIAYGVSETNFKQDAYVTHLWLANVASGKALQLTRGEKSSANPRWSPD
                     GEWLAFTSDRSGDKNQIFVIRPDGGEAVQLTKSETAISNFAWAEDGKSIAYLATEPPP
                     PSLKDRKEHLGDFEVIRREYNFVHLWTLDVAEALNAPVVGKQRTKKRDFSIGSFSWSP
                     DGTRIAFSAQLNPDLIQGVTSDVYLLKLADDSVQKIVSQPGPDNNPSWSPDGKQIVFS
                     SAMGQTVFFHTNNRLALILAEGGTPRSLTDSFDEQPGFIEWNRDGIYFTGLQKTASHL
                     FRVDPSNAKITRVTAPDNLMAGAFNFTRDGRRMAFTATSPTTLNEIFVSDLDRFEPRR
                     LTNMTEQAEAFVLGTREVISWKSKDGAEIEGVLIKPADFDPSKRYPLLCIIHGGPTGI
                     DRPALLAPDARYYPSDIWAARGALILKVNYRGSAGY"
     gene            complement(1530..3098)
                     /locus_tag="VGB76_10990"
     CDS             complement(1530..3098)
                     /locus_tag="VGB76_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099463.1"
                     /translation="MFKRSVSLVLAFIFIAASLALAGQPVPRAASTSEEAKRLVGSAL
                     EAMGGEARWRALKSVGVEGIGHVNAVEQSERPEGPYIVSYEQTNELIDLAGARMRRTV
                     ESRDTQTTQWAARTYIVANGAASAVRGDKAFPGNPLSLQEAELRIALGPERVLLSALA
                     APDLRAEGETVLQGTRQRIVKFTWRDKPVTLFLNAQTHLPTAVETLSAHPYELWSVWG
                     DVRTRVYYSYWTLEAGGLRYPRQWDTERNSLKWRTFTVINLKLNPPTPAESLGIAEDV
                     QKAFKSRPLFTFDTSPLGRPGRPPHDTAPGVYSMPGGWDVTLVRQADGLVVIEAPISS
                     SYSAKVLDEAAKRFPGVPVKAVISTSDAWPHFGGVREYVARGIPVYALDLNRPILERL
                     LAAPRTLNPDTLARTPRKPKFQIVSAKTTIGTGANRLELYPIRTETGERMMMIYFPEH
                     RLLYGSDLLQKMPNGSFFMPQYVSEVMSAAEREKLAVERVFAMHMDAMNWTDVGAAVS
                     KATAETPANDAPKE"
     gene            complement(3205..4020)
                     /locus_tag="VGB76_10995"
     CDS             complement(3205..4020)
                     /locus_tag="VGB76_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0099464.1"
                     /translation="MTRQRNLLLSLLLLLATLQVAFAQDQSDQLFNAARNGDAATVKA
                     LLDRGVDVNTKFRYGATALSYASDKGHLEVVKLLLERKADVNVKDTFYGATPIIWAAQ
                     KGHAKIVEALLDAGAEGKDDALGIGASGGHLELVRTVLAKGGLKAETLSESLVAAERG
                     KYAEIVAALKQAGAQPPPKADFKVDAETLNSYVGTYKLDSGAEFNAVVKDGALVIGPP
                     GQQLTLGAFDKLNFRPLEFEGVTITFNVENGKVTGFTMKRGTNTQVFKKVATP"
     gene            4374..>5182
                     /locus_tag="VGB76_11000"
     CDS             4374..>5182
                     /locus_tag="VGB76_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0099465.1"
                     /translation="MHVPLLKRSALILFTLALAFFAPQTLRAGDWADWRGPARDGVSP
                     EKNLPARWSPAGENLAWKAPYGGRSAPIVMGDRVYLQNTAGKGETLQERILCFHADTG
                     KLLWEHRFNVYLSDVPPHRVGWASPVGDPATGNVYVFGVGGSLLSLSREGKVLWDRSL
                     GEDFGLLTTHGGRTVSPIIDGNLVIVSGVTFIWGEHGRGAHRFMAFDKSTGETVWVSA
                     PGGRPYDTTYATPIIADINGTRLLIQGASDGVVHALKPQTGEPVWKYEISKR"
BASE COUNT          903 a         1551 c         1705 g         1023 t
ORIGIN      
        1 gtagcccgcg ctgccgcgat agttcacctt gaggatgagc gcgccgcgcg ccgcccagat
       61 gtcggaagga taatagcgcg cgtcgggcgc gagcagggcg ggacggtcga tcccagtagg
      121 gccgccgtga atgatgcaga gcagcggata acgcttcgac gggtcgaaat cggcgggctt
      181 gatgaggacg ccttcaatct ccgccccgtc cttgctcttc caggagatga cttcgcgcgt
      241 gccgaggacg aacgcctccg cctgctccgt catgttggtc agacggcgcg gctcgaaccg
      301 gtcgagatcg gagacgaaga tttcattgag cgtcgtggga gacgtggcgg tgaaagccat
      361 gcgccgcccg tcgcgcgtga agttgaacgc cccggccatc aggttgtcgg gcgcggtgac
      421 gcgcgtgatc ttcgcgttcg acggatcgac gcggaagagg tgcgaagcgg tcttttgcag
      481 ccccgtgaaa tagatgccgt cgcggttcca ttcgatgaag ccgggttgtt cgtcgaagct
      541 gtccgtgagg gaacgcggcg tgccgccttc cgccaggatc aaggcgaggc ggttgttcgt
      601 gtggaagaag acggtctggc ccatcgccga ggagaagacg atctgcttgc cgtcgggcga
      661 ccaactcgga ttgttgtcag gacccggctg cgagacgatc ttttgcacgg agtcgtccgc
      721 gagtttgagc aggtagacat ccgaggtgac gccctgaatg agatcgggat tgagttgcgc
      781 gctaaaggcg atgcgcgttc cgtctggcga ccaagagaag gagccgatgc tgaagtcgcg
      841 cttcttcgtg cgctgcttgc cgacgacggg cgcgttcaga gcctccgcca cgtcgagcgt
      901 ccagaggtgg acgaagttat attcgcggcg gatgacctcg aaatcgccga gatgctcttt
      961 gcggtctttg agcgagggcg ggggcggttc ggtggcgaga taagcgatgc tctttccgtc
     1021 ttccgcccac gcgaaatttg agatggccgt ctcggatttc gtcaactgca cggcctcgcc
     1081 gccgtcgggg cgaatcacga aaatctggtt cttgtcgccg gagcggtcgc tggtgaaggc
     1141 gagccattcg ccgtcgggcg accagcgcgg gttcgcggaa gacttctcgc cgcgcgtgag
     1201 ttggagcgcc ttgccgctcg ccacgttggc gagccagaga tgcgtgacgt aggcgtcctg
     1261 cttgaagttg gtttcgctca cgccgtaggc gatccacttg ccgtcgggcg agatgcgcgg
     1321 cgcgcccacg ctcttgagcg cgagcagatc gtcaaccgtc ggcacgcggc gggcggattg
     1381 agcggcgagc ggcagggcgg tcagccagca gagcagcaac gccgagaaag ttgtcgtcag
     1441 ggaggaacgt atattcattg ataaagacac ccggtaatta agctggtttc aagttaccac
     1501 gttggagttt gagactacga aggttgagac tactctttcg gcgcgtcgtt cgcgggcgtc
     1561 tccgccgtcg ccttcgacac ggctgcgccc acgtccgtcc agttcatcgc gtccatgtgc
     1621 atcgcaaaga ccctctccac ggcgagcttt tcgcgctccg ccgccgacat cacttccgac
     1681 acgtactgcg gcatgaagaa cgagccgttc ggcatcttct gcaagaggtc gctgccgtag
     1741 agcaagcggt gttcggggaa atagatcatc atcatccgct cgcccgtctc cgtgcgaatc
     1801 gggtatagct ccagacggtt cgcccccgtg ccgatggtcg tctttgcgga gacgatctgg
     1861 aacttcggtt tgcgcggcgt gcgcgcgagc gtgtccgggt tgagcgtgcg cggtgcggcg
     1921 agcagacgtt cgaggatggg gcggttgagg tcgagcgcgt agacggggat gccgcgcgcc
     1981 acgtactcgc gcacgccgcc gaaatgcggc cacgcgtcgg aagtcgagat gacggctttg
     2041 acgggcacgc cggggaaacg cttcgcggct tcgtcgagca ccttcgcgga gtaactcgaa
     2101 gagatcggcg cttcgatcac gacgagtccg tccgcctgtc gcacgagcgt cacgtcccaa
     2161 ccgccgggca tcgaatagac gccgggcgcg gtgtcgtgcg gcgggcgacc gggtctgccg
     2221 agcggcgacg tgtcgaaggt aaagagcggg cgcgacttga aagccttctg cacgtcttcg
     2281 gcgatgccga gcgactcggc gggcgtgggc gggttgagtt tgagattgat gacggtgaac
     2341 gtgcgccact tcaaactatt tcgttcggtg tcccactggc gcgggtagcg gaggccgccc
     2401 gcttcgagcg tccagtagga ataatagacg cgcgtgcgca cgtcgcccca gaccgaccac
     2461 agttcataag ggtgcgcgct taaggtttcc acggcggtcg ggagatgcgt ctgcgcgttg
     2521 agaaagagcg tcacgggctt gtcgcgccag gtgaatttca cgatgcgctg ccgcgtgccc
     2581 tgcaacacgg tttcgccttc ggcgcgcaag tccggcgcgg cgagcgcgct cagcagcacg
     2641 cgttcgggtc caagcgcgat ccgcagttcg gcctcctgca agctgagggg gttgccgggg
     2701 aaagccttgt cgccgcgcac tgcgctcgcc gcgccgttgg cgacgatata ggtgcgcgca
     2761 gcccattgcg tcgtctgtgt gtcgcggctc tcgacggtgc gccgcatcct cgcgcccgcg
     2821 aggtcgatca actcgttagt ctgctcgtaa gagacgatgt aaggaccttc gggacgctcc
     2881 gactgttcga ccgcgttgac gtgcccgatg ccttcaaccc cgacggactt caacgcacgc
     2941 caacgcgcct cgccgcccat cgcctccaac gccgacccta cgaggcgctt cgcctcttcg
     3001 gaggttgaag ccgcacgggg cacgggttgc ccggcaagcg cgagcgacgc ggcgatgaag
     3061 atgaaggcga gcactaaaga gactgaacgt ttgaacacga tggggaagac tccttttcga
     3121 gatgttaatt cactaacgac agaacgctcg attgaattaa caatgcgacg cgccgaacct
     3181 gccgggcttg agcagggcgc ggcgttacgg cgtcgccact ttcttgaaga cctgcgtatt
     3241 cgtgccccgc ttcatggtga agccggtgac tttgccgttc tcgacgttga aggtgatcgt
     3301 cacgccttca aactcaagcg ggcgaaagtt gagtttgtcg aacgcgccga gagtgagttg
     3361 ctgtccgggg ggtccaatga cgagcgcgcc gtccttcact acggcgttga actccgcccc
     3421 gctgtccagc ttgtacgtcc cgacgtaaga gttgagcgtt tcggcgtcaa ccttgaagtc
     3481 ggctttcggc ggcggctgcg ctccggcctg cttgagcgcg gcgacgatct ccgcgtactt
     3541 gccgcgctcg gcggcgacga gcgactcgga gagcgtctcg gccttcaagc cgcccttggc
     3601 gagcaccgtg cggacgagtt ccagatggcc gccgctcgcg ccgatgccga gcgcgtcgtc
     3661 cttcccttcc gcgcccgcgt cgagcaacgc ttcgacgatc ttggcgtgcc ctttctgcgc
     3721 cgcccagatg atcggcgtcg cgccgtagaa cgtgtctttg acgttcacgt cggccttgcg
     3781 ttcgagcagc agtttgacca cttccagatg ccctttgtcc gaggcgtagg agagcgcggt
     3841 cgcgccgtaa cgaaatttcg tattcacatc gacgccacgg tcgagcagag ctttgacggt
     3901 cgctgcgtcc ccgttgcgtg cggcgttgaa cagttgatcg ctctggtctt gtgcgaaagc
     3961 gacctggagc gtggcgagta ggagcaggag agacaacaaa agatttctct ggcgcgtcat
     4021 aggttcacct caaggcgtag ctgctgttgt cggaggattt cggaactaca ttttccggcg
     4081 tttacatttt ttcggatcgc gggttgtact tttttggatt gcagcgatgg cggcacggcg
     4141 cgtccttgcg gtgcgtcgcc gccgaaaatg aagtttgccc gtaggtttta cgtctaaccc
     4201 ggcgggtagg ttgcaaggag cagtttgcct cgctcaataa agcgcgacat aatatgtggc
     4261 gcactccggt ttgagtcaag aaaaacttcc ggcgggcggc aacttaaact tttcaagtgc
     4321 gtatttctac agaagcattg caatactatc aaccgcctaa ggtaaaactg atcatgcacg
     4381 tccctctctt gaaacgctcg gctctcattt tgtttacact cgcgctcgcc ttcttcgcgc
     4441 cgcagacgct gcgtgcgggc gactgggcgg actggcgggg tcctgcgcgc gacggcgtct
     4501 cgcccgaaaa gaatctgccc gcgcgctggt cgcctgcggg cgagaacctc gcctggaaag
     4561 ctccttacgg cggtcgctcc gcgcccatcg tcatgggcga ccgcgtgtat ctacagaaca
     4621 ctgcgggcaa gggcgagacg ttgcaggagc gcatcctctg tttccacgcc gacacgggaa
     4681 aacttttgtg ggagcatcgc ttcaacgtct atctctcgga cgtgccgccg catcgcgtcg
     4741 ggtgggcttc gcccgtgggc gacccggcaa cgggcaacgt ctatgtcttc ggcgtcggcg
     4801 gctcgctcct gtccctgtcg cgcgagggta aagtgctatg ggatcgttcg ctcggagaag
     4861 acttcggact cctgaccacg cacggcgggc gcaccgtctc tccgattatc gacggcaacc
     4921 tcgtgatcgt ctctggcgtc acgttcatct ggggcgagca cggacgcggc gcgcaccgct
     4981 tcatggcctt tgacaagagc actggcgaaa ccgtctgggt gagcgcgccc ggcggtcgcc
     5041 cctacgacac gacctacgcc acgcccatca tcgccgacat caacggcacg cgcctgctga
     5101 ttcagggcgc aagcgacggc gtcgtccacg cgctcaaacc gcagacgggc gagccggtct
     5161 ggaagtacga gatttcgaag cg
//
LOCUS       DASWNS010000315         8133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_63515, whole genome shotgun sequence.
ACCESSION   DASWNS010000315 DASWNS010000000
VERSION     DASWNS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63515"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VGB76_11005"
     CDS             <1..823
                     /locus_tag="VGB76_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099466.1"
                     /translation="YARRSPSELLENAAGENISGRRASHAPAQLVRASFAHANFAHAS
                     FAPARVAHSQSSVARANVVASPPAQTSARLTLSSANFRVSYPARGASASRREVESVLA
                     VLEGVRADVRARLAAASLAFPALGVLDVTVHETTGDFTGATGKPAWVAATTTTRGARR
                     IELQPLATLGRRGVLASTLRHEYAHAVIHALSRARAPLWLSEGLAAHVANEGARLTRF
                     ESKRKLSLADLENKLAQPAASDIEMRALYAAAFREVIALIRAEGEANVWRRVARG"
     gene            915..2135
                     /locus_tag="VGB76_11010"
     CDS             915..2135
                     /locus_tag="VGB76_11010"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0099467.1"
                     /translation="MSYTIEPETTEAASPLDHTLTPHFHALAQFHSTKTLAASSLYRE
                     LRPEIERVLGHVVQEEFAAPETRTRAPNAAATIRATAWNIERGKRLEGIKHALREHPV
                     LRASDVLLLTELDYGMARTGNADVAREIARTLGMNYAFAPCYLALNKGSGVEADVEGD
                     NAQALHGNALLSRYPLRRVHSLALPNGKDKMAGKEKRLGQQRAVVADVEHPHGMFRAV
                     SLHLDAHSTQRHRYQQMRLVLDHLDGLAPRLPTLIGGDWNTSTYNSRRALYSILGYAR
                     RVAMGVENVVKNHYPYPERWFERKLFRALERRGYSYRELNALGVCTLHYDVGDATVNT
                     NMGDWIPQWCFWFINWALKKHEGRCSLKLDWFAGKKIKLAAGARPQVVGELRDAEGVL
                     SDHDPIVLDFSLES"
     gene            complement(2155..2787)
                     /locus_tag="VGB76_11015"
     CDS             complement(2155..2787)
                     /locus_tag="VGB76_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEY0099468.1"
                     /translation="MFAPDINFTRIVVFTNGLVPALLLTWDAYQQKLGANPIEFATRA
                     TGALTLVFLFITLAVTPLRKLTGANWLIKFRRMLGLYSFFYGSLHLLTYVWFDKFFSG
                     REIIEDVTARPFITIGMASFLLLVPLAVTSTNGMIKRLGGKRWAKLHKLTYVAATGGL
                     VHYYMAVKADTTIPILFAIMLAVLFGYRWVKSRLDATTKNDTLGIQTPPR"
     gene            complement(2802..3785)
                     /gene="msrP"
                     /locus_tag="VGB76_11020"
     CDS             complement(2802..3785)
                     /gene="msrP"
                     /locus_tag="VGB76_11020"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEY0099469.1"
                     /translation="MLIRKPDDIKSSEITDKKLYLTRRNFMRGAALAASTVATGFIYR
                     QFAVPPPPPVKGAKIENLQTVEQGAPGFPPPPGEALTSYEEITNYNNFYEFSTGKSEV
                     ASVARNFSTRPWMVSVGGLVQRPREFDLDDLLKIAPPEERVYRMRCVERWSMVIPWVG
                     FPLRKLLEQVEPLGHARYVAFQTLHDPRQMPNQRRDVLEWPYVEGLRLDEALHPLTIL
                     ASGLYGETLPPQNGAPLRLVVPWKYGFKGIKSIVRITLTDRQPPATWNISAPDEYGFY
                     SNVNPKVHHPRWSQAKERRIGEFSLRDTLPFNGYADQVAMLYAGMDLRVNF"
     gene            complement(4195..5664)
                     /locus_tag="VGB76_11025"
     CDS             complement(4195..5664)
                     /locus_tag="VGB76_11025"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEY0099470.1"
                     /translation="MRKLNELMFDNTYARLPEAFYSKVNPTALLEPHLVSFNAAAAEL
                     IDLDPREAERAEFVEYFSGSRTLPGAEPLAMLYAGHQFGSYVSQLGDGRAILLGEVRN
                     ARGAKWDLHLKGAGQTPYSRGFDGRAVLRSTVREYLCGEAMHALGIPSSRALCIVGSD
                     EPVRRETIESAAMLVRMSESHVRFGTFELFYYRGQQERVRELADYCIVNHFPELHESP
                     DKYRLFLLEVARRTARLIAKWQAVGFAHGVMNTDNMSILGLTLDYGPFGFLDDYQAGF
                     ICNHSDYSGRYAFDQQPGVGLWNVSRLAQALVNLVTVEEATAALDTYQPAFAAHYGEL
                     MRAKLGLATESDTDAELLLSLLGLLEANGVDYTNFFRRLGNFRTADDSPQDELRDMFI
                     EPAAFDAWAARYRARLLAETSDDDERKSRMDRVNPKYVLRNYLAQQAIVAAVERRDYT
                     EIERLLELLRAPFAEQPGAERYAAPPPDWGKRLSISCSS"
     gene            complement(5962..7140)
                     /locus_tag="VGB76_11030"
     CDS             complement(5962..7140)
                     /locus_tag="VGB76_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0099471.1"
                     /translation="MMTEVKRVKPDIRLITRRVQPDGNLVQGQSELAIDARLARAAGE
                     MIAASQNHYSPAEGAKTLRHAVAEKIRMFNGIEVDPDASPLELIITPGATGGLVAIAD
                     AYLKNASALIFEPYYPYHRRIIEELGGRAEVLQLTGESLQFDREELRARCRELRSRRE
                     FPLRAIIVCTPVNPTGKVFTRAELTAIAEVCQEFELLCISDEVYEHYVADDGEPHVSI
                     AGLAGMRERTVTVNSFSKSWNISGWRLGYAYGDAKLVAPLNNANNVFYVCSPTPLQKA
                     LAEVLMADTDYYARLGERFKAKRARVTGVLEELGFRIYDSGSTFYVWARIPEEHGDAM
                     RLNEKLIAQAGVAAVPGSAFTDSDVWDNYMRLCIAREDHILDVALSKLHGALTRGLSD
                     "
     gene            complement(7243..7383)
                     /locus_tag="VGB76_11035"
     CDS             complement(7243..7383)
                     /locus_tag="VGB76_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099472.1"
                     /translation="MSKRGIPFIALGTAFLGIGVASQRTFIYVGIAFLIIGIILLRRT
                     RH"
     gene            7573..>8133
                     /locus_tag="VGB76_11040"
     CDS             7573..>8133
                     /locus_tag="VGB76_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY0099473.1"
                     /translation="MPKKNSPNSVTVGLVQMTCAPEPAVNMKKAIARIGEAAKRGAQV
                     VCLQELFRSQYFCQTEDIELFKLAETIPGPSTEALSKVARQHKIVIVASLFEKRAAGL
                     YHNTAVILDSDGQIAGKYRKMHIPDDPLYYEKFYFTPGDLGFASHDTGVGKIGTLVCW
                     DQWFPEAARLTALSGAQFLFYPTAIGW"
BASE COUNT         1562 a         2522 c         2514 g         1535 t
ORIGIN      
        1 ttacgcgcga cgctcgccct ccgagttact tgagaacgct gctggcgaaa atatttcagg
       61 gcgtcgcgcg tcgcacgcgc ccgcgcaact cgtgcgagca agtttcgctc acgccaattt
      121 cgctcacgcc agtttcgcgc ccgcacgcgt cgcccattct caaagcagcg tagcgcgcgc
      181 caacgtcgtc gcttcgccac cggctcagac gtcggcgcgt ctcactttgt cgagtgcaaa
      241 tttccgcgtg agttatccgg cacgtggcgc gtcggcttcg cgtcgtgaag ttgagagcgt
      301 gctcgccgtg ctcgaaggcg tgcgcgcgga cgtgcgcgca cgtttggctg cggcctcgct
      361 cgccttcccc gcgctcggcg tgctggacgt gacggtgcat gagacgacgg gcgacttcac
      421 gggcgcgacg ggcaagcccg catgggtcgc cgcgacgacc acgacgcgcg gcgcgcgccg
      481 catcgaactg caaccgctcg ccacgcttgg ccgtcgcggc gtgctcgcgt ccacactccg
      541 gcacgaatac gcgcacgcag tgatccacgc gctctcgcgc gctcgcgcgc cactctggct
      601 ctccgaagga ctcgccgcgc acgtcgccaa tgaaggcgca cggctcacgc gtttcgagtc
      661 gaagcggaag ttatcgctgg cggatttgga gaacaaactg gcgcagcccg ccgcgtccga
      721 catagagatg cgcgctctct acgccgccgc ctttcgcgaa gtcatcgcgc tcatccgcgc
      781 ggaaggcgag gccaacgtgt ggcgtcgcgt cgcgcgcggt taagcggtga ccaagttgca
      841 gaggcgttaa agtttttaaa ttcgatcacg ccaatttgat catagacgct tcccgcctgt
      901 aacatctccg ggctttgagt tacaccatcg aaccagaaac gacggaggcg gcttcgcctc
      961 tcgaccacac gctcacgccg cacttccacg cgctcgcaca gtttcattca accaagacgc
     1021 tcgccgcctc gtctctctat cgagaactgc gcccggagat cgagcgcgtg ctcggccacg
     1081 tcgtacagga agagttcgcc gcgccggaaa cgcgcacgcg tgcgccaaac gcggcggcca
     1141 ccattcgcgc gaccgcgtgg aacatcgagc gcggcaaacg tctggaaggc atcaagcacg
     1201 cgctccgcga gcatcccgtc ctgcgcgcga gcgacgtgct gttgctcacc gagttggact
     1261 acggcatggc gcgcacgggc aatgccgacg tggcgcgcga gatcgcgcgc acgctcggaa
     1321 tgaattacgc cttcgcgccc tgctatctcg cactgaacaa ggggagcggc gtagaggctg
     1381 atgtcgaagg tgataatgcg caggcgttgc acggcaacgc gctgctttcg cgctaccctc
     1441 tgcgacgcgt ccactcgctc gcgctgccga acggcaagga taagatggcg ggcaaagaga
     1501 agcgtctggg gcagcagcgc gccgtcgtcg cagacgtgga gcatccgcac ggcatgtttc
     1561 gcgccgtcag cctgcacctc gacgcgcact ccacgcagag acaccgctat caacagatgc
     1621 gcctcgtgct cgatcatctc gacggcctcg cgccgcgcct gcccacactc atcggcggcg
     1681 actggaacac ttcgacctac aactcgcgtc gcgccctcta ctcgattctc ggctacgcgc
     1741 gtcgcgtcgc aatgggcgtg gagaacgtgg tcaaaaatca ttacccgtac ccggagcgtt
     1801 ggtttgagcg aaaacttttt cgcgcgctgg agcggcgcgg ctacagctat cgcgagttga
     1861 acgcgctcgg cgtctgcacg ctgcactacg acgtgggcga cgcgacggtg aatacgaaca
     1921 tgggcgactg gattccgcag tggtgtttct ggttcatcaa ctgggcgctc aaaaaacatg
     1981 aaggccgctg ctcgctcaag ctcgactggt tcgcgggcaa aaaaatcaaa ctcgccgcag
     2041 gcgcgcggcc acaggtcgtc ggcgaactgc gcgacgcaga gggcgtgctc tcggatcacg
     2101 atccgattgt gctggatttc agtctggaga gttgaaggct cgcgcggcgg agtttcaacg
     2161 cggcggagtt tgaatgccga gcgtgtcgtt tttggtcgtc gcatccagac gcgacttgac
     2221 ccaacgatag ccgaacagca cggcgagcat gatggcgaac aggatcggga tcgtcgtgtc
     2281 ggccttcacg gccatgtagt agtggacgag gccgccggtg gccgccacgt aagtcagctt
     2341 gtgcagtttc gcccaacgct tgccgccgag ccgtttgatc atgccgttgg ttgaagtcac
     2401 tgcgagcggc acgagcagca ggaaactcgc catgccgata gtgatgaacg ggcgcgcggt
     2461 aacgtcttcg atgatctccc gcccgctgaa aaacttgtcg aaccacacgt aggtcaagag
     2521 atgcagggag ccgtagaaga aactgtagag gccgagcatg cggcgaaact tgatgagcca
     2581 gtttgcgccc gtgagtttgc gtaaaggggt gacggcgagc gtgatgaaga ggaagacgag
     2641 cgtgagcgcg cccgtggctc gcgtcgcaaa ctcgatgggg ttcgcgccga gtttctgttg
     2701 ataagcgtcc cacgtgagca gcagcgcggg cacgagtccg ttcgtgaaga caacgatgcg
     2761 tgtgaagttg atatcgggcg cgaacattcg atctccccgc gttaaaagtt gacgcgcaag
     2821 tccatccccg cgtagagcat cgccacctga tccgcgtagc cgttgaaagg caacgtgtcg
     2881 cgcagagaga actcgccgat gcgccgctcc ttcgcctgac tccagcgcgg gtgatgaact
     2941 ttcggattca cgttcgaata gaacccgtac tcgtccggcg cggagatatt ccacgtcgcg
     3001 ggcggctggc gatccgtgag cgtgatccta acgatggact tgatcccctt gaagccgtac
     3061 ttccacggca cgaccagacg cagcggcgca ccgttttgcg gcggcagcgt ctcgccgtaa
     3121 aggccgctcg cgaggatggt gagggggtgc agggcttcgt ccagacgcaa gccttcgacg
     3181 taaggccatt cgagcacgtc gcggcgttga ttcggcatct gcctcggatc atggagcgtc
     3241 tggaaggcga cgtatctggc gtggccgagc ggttcgacct gctccaataa tttccgcagc
     3301 gggaacccga cccacgggat gaccatcgac caacgctcca cgcagcgcat gcgatagaca
     3361 cgctcctcgg gcggcgcgat cttcaagaga tcgtcgaggt cgaactcgcg cggcctctgc
     3421 accagacctc cgaccgatac catccacggt cgcgtcgaga agttgcgcgc cacgctcgcc
     3481 acttccgatt tgcccgtcga gaactcgtag aaattgttgt agttcgtgat ctcctcgtag
     3541 gaggtcaaag cttcaccggg gggcgggggg aaacccggcg cgccctgctc taccgtctgc
     3601 aagttttcga tcttcgcgcc cttcacgggc ggcggcgggg gaacggcaaa ttgacgatag
     3661 atgaatccgg tggcgaccgt gctggcggcg agcgcagccc cgcgcatgaa gttgcgtcgc
     3721 gtgagataca atttcttgtc cgtgatctcg ctgctcttga tgtcgtccgg cttccttatc
     3781 aacatctgcg ctccactctc cgtccctaac taaaacttcc cctaaaactt cgcagcctgc
     3841 gcttcgctac aacggaaata cgcgtgccgc ttcgactccg gatgtaacgg cggggatact
     3901 gaaaactcta cgagcctctg cgccataatg caaaggcaca cgcttcgccc gcgcttttta
     3961 tcgcgcgggc gaagcgtgtg cctcgaagtt atgctggcgg ctccgcgcct ctatttttgc
     4021 gcttaccagc gcggacgagg cgagcgttta agtgtcggaa taaacgggtt gggcggcggc
     4081 gggcggaagt tacgcttaca cgaaaacggc gcgcggctca tcgtatcgtg tgcggcgaaa
     4141 tcgaggcgca cggaggcagt tatcaactcg cggcggcgaa aaaaattctc gcttttatga
     4201 agagcaacta atggagagcc gcttgcccca atcgggtggc ggcgcggcgt agcgttccgc
     4261 tccgggctgt tccgcgaatg gggcgcgaag cagttcgagc aatcgttcaa tctccgtgta
     4321 gtcacgccgc tcgactgccg cgacgatggc ctgttgcgcc agataattgc gcagcacata
     4381 tttcggattg acgcgatcca tgcggctctt tcgctcgtcg tcgtcgctcg tctcggcgag
     4441 caggcgtgcg cggtagcgcg ccgcccacgc gtcgaaggcg gcaggctcga tgaacatgtc
     4501 gcgcagttcg tcttgcggcg agtcgtccgc tgttctgaaa ttgccgaggc ggcggaagaa
     4561 gtttgtatag tccacgccgt ttgcttcgag caggccgagc aggctcagga gcaactccgc
     4621 gtccgtatcg gactcggtgg cgagtcccag cttggcgcgc atcagttcgc cgtagtgcgc
     4681 ggcgaaggcg ggctggtagg tgtcgagcgc ggcggtcgct tcttccaccg tgaccaggtt
     4741 cacgagtgcc tgcgcgagcc gactgacgtt ccagagtccg acgccgggct gctggtcgaa
     4801 agcgtagcgt cctgagtagt ccgaatgatt gcagatgaat ccggcttggt aatcgtcgag
     4861 gaagccgaac ggcccgtagt cgagcgtgag gccgaggatg gacatgttgt ccgtgttcat
     4921 cacgccgtgc gcgaagccga ccgcctgcca cttcgcgatg agccgcgccg tgcgccgcgc
     4981 aacttcgagc aggaacaggc gatacttatc cgggctttca tggagttcgg gaaaatgatt
     5041 gacgatacaa taatcggcta actccctgac gcgttcctgc tggccgcgat aataaaagag
     5101 ttcaaacgtg ccgaagcgga catggctctc cgacatgcgc acgagcatcg ccgccgactc
     5161 tatcgtctcg cgccgcaccg gctcgtcgct cccgacgatg cagagcgcgc ggctggaagg
     5221 tatgccgagc gcgtgcatcg cctcgccgca taaatattcg cgcacggtcg agcgcagcac
     5281 tgcgcgcccg tcgaacccgc gcgagtaagg tgtctgtccc gcgcccttca aatgcaagtc
     5341 ccatttcgcg ccgcgcgcat tcctcacttc gcccaacagg atcgcgcgcc cgtcgccgag
     5401 ttgcgatacg tagctgccga actggtgccc cgcgtacagc atcgcgagcg gctccgcgcc
     5461 gggaagcgtc cggctgccgc tgaaatattc cacaaactcc gcacgctccg cctcgcgcgg
     5521 gtcgagatca atcaactccg ctgccgccgc gttgaagctg acgagatgag gctcaagaag
     5581 agcggtcggg ttaactttgg aatagaaggc ttcgggcagg cgcgcgtagg tgttgtcgaa
     5641 catcaactcg tttaatttgc gcatcgaaac gtctcccccc ttgaactatt gaacgctacg
     5701 atagaagaat cacaaagttt gcgccacccg gcagacgacg caacgcgcca ggagagcgac
     5761 aggcagacgg ctcgaccgca aggcttctca cgccggatag gtgcgggctg cgccgacgac
     5821 acgccttcac aagttgaatt tcaggatcaa cagaaagacc gtcgtgatga aaatgatcgc
     5881 cagataacag gtcgccaaca ctgcacgcag gccgcgccca tttgcacccc acaatcgcaa
     5941 gctgcccagg tcgcgtctcg cctagtcgct caggccgcgc gtgagcgcgc cgtgcagttt
     6001 ggagagcgcg acatcgagaa tgtgatcttc gcgcgcgatg cagaggcgca tgtagttgtc
     6061 ccacacgtcg ctatccgtga acgcgctgcc cggcacggcg gcgacgcccg cctgcgcgat
     6121 caatttttcg ttgagccgca tggcatcgcc gtgctcttcg gggatgcgcg cccagacgta
     6181 gaaggtcgag ccggaatcgt agatgcgaaa gcccaactct tcgagcactc cggtgacgcg
     6241 cgctcgcttc gccttgaagc gttcgccaag acgcgcgtag tagtccgtgt cggccatcaa
     6301 cacttcggcg agagcttttt ggagcggcgt cggcgagcag acgtagaaga cgttgttggc
     6361 gttgttgagc ggcgcgacga gtttggcgtc gccgtaagcg tagccgagcc gccagccgga
     6421 gatgttccag gacttcgaga aagagttgac cgtgacggtg cgctcgcgca tccccgccaa
     6481 gcccgcgatg gagacgtgcg gttcgccgtc gtccgccacg tagtgttcgt agacttcatc
     6541 cgagatgcag agcagttcaa actcctgaca gacctccgca atcgcagtca gttcggcgcg
     6601 cgtgaaaact ttgccggtcg ggttgacggg cgtgcagacg atgatcgcgc gcagaggaaa
     6661 ctctcgacgc gatctcaact cgcggcaacg cgcgcgcaac tcttccctgt cgaactgcaa
     6721 agactcgccc gtcaattgca agacctccgc gcggcctccg agttcttcga tgatgcgccg
     6781 gtgataagga tagtaaggct cgaagatcag tgcggaagcg tttttcagat acgcgtcggc
     6841 gattgcgacg aggccgcccg tcgcgcccgg cgtgatgatc aattcgagcg ggctggcgtc
     6901 cgggtcaact tcgatgccgt tgaacatgcg aatcttttcg gccaccgcgt ggcgcagagt
     6961 cttcgcgcct tcagccgggc tgtaatgatt ctggctcgcg gcgatcatct cgcccgccgc
     7021 acgcgcgagc cgcgcgtcaa tcgccaactc ggactgcccc tgcacgagat tgccgtccgg
     7081 ctgaacgcgc cgcgtgatca atcgaatgtc gggcttaact cttttcacct cggtcatcat
     7141 aagcagtctc ctttgagggg cgcaggcgaa gttcggcggc gaacattcct tgcgagtctt
     7201 gagtccggaa gcctgtgcgg gagggccagc gttgtgcgcg cttcaatggc gcgtgcgtct
     7261 gagcaaaatt attccgatga tgagaaacgc gatgccgacg tagatgaaag tgcgttggct
     7321 ggcgacgccg attccgagaa aggccgtgcc cagcgcgata aaagggatgc cgcgtttcga
     7381 catgttggac gcctccgttt gaggggttcg ctgaatttgt taaccgacgt gcgctctaaa
     7441 gatacttttg aaccgggaaa gtttcaacgg cgcacggcgg gcttctcgcc cctccgctga
     7501 gttggccgtc aaaattcgca cgcacgcggt ttgaattaaa gtttcgcggg cggctttgct
     7561 atactcgccc gcatgccgaa aaagaattct ccgaacagcg tcacggtggg gctggtgcag
     7621 atgacttgcg cgcccgagcc tgcggtcaac atgaagaagg cgattgcgcg cattggcgag
     7681 gcagccaaac gcggcgcgca ggtcgtctgc ctgcaagagc ttttccgctc gcagtatttt
     7741 tgccagacgg aagacatcga actcttcaaa ctggccgaga cgatccccgg cccctcgacc
     7801 gaagccctct cgaaggtcgc gcgccagcac aaaatcgtca tcgtggcctc tctgtttgag
     7861 aagcgcgccg cagggcttta tcacaacacg gctgtcattc tcgactccga cggccagatc
     7921 gcgggcaagt atcgcaagat gcacatcccg gacgacccgc tctactacga aaaattctac
     7981 ttcacgcccg gcgacctcgg cttcgcaagc cacgacaccg gggtcggcaa gatcggcacg
     8041 ctcgtctgct gggaccagtg gtttcccgaa gcggcgcgcc tgaccgcgct ctccggcgcg
     8101 cagtttctct tctacccgac tgccatcggc tgg
//
LOCUS       DASWNS010000316         7964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_221453, whole genome shotgun sequence.
ACCESSION   DASWNS010000316 DASWNS010000000
VERSION     DASWNS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221453"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VGB76_11045"
     CDS             complement(<1..337)
                     /locus_tag="VGB76_11045"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0099474.1"
                     /translation="MWYAVELKIERAAEEAAEYALMEAGALGTETTEEAGTMRVAGYF
                     ASAPEREAVRGFVLDALRVYALPAGSVGELEVREVAARDWLGEWKKSWQPVEVGTRFV
                     IAPPWSEVEE"
     gene            complement(551..1456)
                     /gene="era"
                     /locus_tag="VGB76_11050"
     CDS             complement(551..1456)
                     /gene="era"
                     /locus_tag="VGB76_11050"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY0099475.1"
                     /translation="MSEQANYRSGYVALIGRPNAGKSTLLNRLVGEKIAAVSNKPQTT
                     RYQIQGIITRPEGQIVLVDTPGVHQPGYLLNRRMMAAVHDALEGVDLVLLMRDASVPT
                     GNGDRFVLDLVKESGKPALLLLNKVDRVGDKAKLLPLMQWYAGEYDWREIVPLSARTG
                     DMVDTLLAGIVKHLPEQEPLFAEDELTDQSTRALCAEIVREKILQTTGDEIPYVTAVL
                     TERFDEEREDFTRINCIIYVERASQKAIIIGRAGERIKEIGTQARRDIEQLLGHRVHL
                     ELFVKVQEDWRNSERALNEMGIKEQ"
     gene            complement(1551..3305)
                     /locus_tag="VGB76_11055"
     CDS             complement(1551..3305)
                     /locus_tag="VGB76_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099476.1"
                     /translation="MPAPSIEQLLDRLDELKRPGGDPRERARLERVLRQATRRSFTEA
                     ETLIRFHEITLYLRAYPQSASLLAHTEKILDSFAARVERLRASGADLTPFEQPDVSGI
                     AATGFTAVFGYDITRWLARTHPRRVSLVWDEEADHAHLNLVWPRLLPLFEEDAYVDAH
                     APALAWLRAAKRKGESDLAWLVRAFERLPTGDKARAELFEPLKLWVRWELGKSKATRT
                     LMRRRRRAREIFYHDAPLLGRREVSLAHELDAPGRRLRVERLSRAEGERLLASGRDTM
                     AVRFRELHGFTHGDPERVLRAEAGRGLEIFLWGVRPERRLPLLAYHCGIFFKNGVPMG
                     YHEGLSLFERVEIGLNLFYTFRDGESAWAYARLMRLYKQVSGVDTFSIDPYQLGGSGN
                     EEGIASGAFWFYRKLGFRPTREELAKLLATEERRLAARRDYRTPPRLLRRLASGHMLF
                     ETRDATATPPPGAWDSFQVRNIGLAINRRMARELDGEAQAIRDASVKAVGRALGANRE
                     PFNEAEERAFSNFALVLALVPDLARWTREEKRDALAVIRAKAGADERRYLRLLCRHAR
                     LRRAIIALGTNKAVTSNR"
     gene            complement(3560..4714)
                     /locus_tag="VGB76_11060"
     CDS             complement(3560..4714)
                     /locus_tag="VGB76_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0099477.1"
                     /translation="MINQERIKNLLLELVQIDSVSRKERDVAERIKKYCEEMGATVEI
                     DDAGEKVGGNSGNVIARFPGTIPDAETIMMSAHMDTVVPGEGVKPIVEGDIIRSDGTT
                     VLGGDDKSGCAVILETIRCLQEQNIPHAPIEAVFSICEEVGLLGAKHVDTSKLKAKYG
                     LVFDSDDPGFLFTKGPSADHMDFKIYGLESHAGVAPEEGISAIRIAAEAIHNMKLGRI
                     DHETTANIGVIEGGKATNITTNFVLLRGEARSLDDKKLEAQTAHMIKCLEDAAAKWEV
                     TVAGVTTKGRVESHVTREYSGMDVPDSSRVVQLVYKAAARLGQKVETLASGGGCDANI
                     FNKRGIECANFGTGMRAIHTVKEWLDVKDLYASAEMTLEVLKVNGEMAQK"
     gene            complement(4823..5632)
                     /locus_tag="VGB76_11065"
     CDS             complement(4823..5632)
                     /locus_tag="VGB76_11065"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0099478.1"
                     /translation="MQVSLDKGEEPEGESPPARRETHAPDGGQTVSRRLEKTPTHADE
                     PSPPARADRFASLSAYSFRQRFVIRAADLAFYVLINLVGRTARFEVEGWEHWEAATRG
                     GKQPIYTFWHNRVFLGTYFFRRRGIVVMTSRSFDGEYIARFIQRFGYGAARGSSSRGA
                     VGALIEMIKLMRQGRPGGFTIDGPRGPRYVAKMGAVLLAKKTGAPILPFTVDAARYYA
                     APTWDALQIPYPFTRARVRLAPLIHVSADADEAALFAKRDELQRALDALND"
     gene            5867..6766
                     /locus_tag="VGB76_11070"
     CDS             5867..6766
                     /locus_tag="VGB76_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099479.1"
                     /translation="MDKRRILIVDANKELRTLLSQVLGEFGHEVVATGNRDDALARED
                     LEDFDLIISDLTEDAEGNVQVLSEIKRRRLVVPVVVSSEDSQHPGLVKAFKMGAANYL
                     RRPYDKQELQAIVEKTLGYKLRFVEDMKVVPFVRERIEFELPSDLSLMNGVLHYLHER
                     ASKLGLINPERSNLFIALDEAFVNAVKHGNKYDQKKLVRITADLSPKEARFTIEDEGE
                     GFNVKDVPDPRDPANLFRTSGRGVLLIYNIMDEVAYNERGNRLTMVKRPEESFDHKLI
                     DSLTPEDHDRRRRTRRRPTSNSG"
     gene            6693..>7964
                     /locus_tag="VGB76_11075"
     CDS             6693..>7964
                     /locus_tag="VGB76_11075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0099480.1"
                     /translation="MTRSLPKTTTAADARAAARPLTADDVSLAPPAAASVVATATHVD
                     DSRDSGALPTRQAWRWWLPPTLSALLFTIYFIDPFAGDWDALDYTVHAVRGEPSSMLF
                     GRMLFIFINHAAYRVAHALFGLPSEQAYLLFKYMVVVQTPFAVVACWTLARELSGSLR
                     TATVAAFMLALSPFFIVYSGQAMTETPSILLLSIALITHLRGVRARVNWQVLAGAALL
                     GLCVNIREPVGFFGVWLVLAPHCCYGWRLQRRELFLTALACALFLTCALAPFALWWLL
                     DVRGYRANWYGWLASMRAESAVHPVKLSNLVPFLQLFFIAAPATFLALPFALQREWRE
                     GRKATPIFVMALVGLFVQLMLLANYSTVLNGRYQLTGLPGLFPLTAAYLLRRTSERTR
                     RRRSSTSSNSRSPRRALVYTCAVVACASLLTG"
BASE COUNT         1385 a         2510 c         2459 g         1610 t
ORIGIN      
        1 tctcttcgac ctcgctccat ggcggcgcga tgacgaaccg cgtccccacc tcgaccggct
       61 gccaactctt cttccactcg cccaaccaat cgcgcgccgc cacctcgcgc acttccaact
      121 cgcccacgct cccggcaggc aacgcgtaga ccctcagcgc atcgagcacg aaaccacgca
      181 ccgcctcgcg ttccggcgcg ctggcgaagt atccggcgac gcgcatcgtg cccgcttctt
      241 ccgtcgtctc cgtccccaac gcgcccgctt ccatcaaagc gtattccgcc gcttcctccg
      301 ccgcgcgctc aatttttaac tcaaccgcat accacataag aacagtgaca agtgaacaag
      361 tgacaggtga cgagtaagca ccagttaaat caggaacgag ttaaaagcgt cgccctggtt
      421 tatgactttc gtgcgggtga caagcgacga gcaggaagca acaagcagga agcgacaagc
      481 cgagcaggaa gcgacgagat gttaacccag cacttgtcac tgtctctctt aacttgtcac
      541 tcgtcactcg tcactgttcc ttgattccca tttcgtttaa ggcgcgctcg ctgttgcgcc
      601 agtcctcctg aactttgacg aagagttcga ggtgtacgcg gtggccgagg agttgttcga
      661 tgtcgcggcg tgcctgcgtg ccgatctctt tgatgcgctc gcccgcgcgc ccgatgatga
      721 tggccttttg cgatgcgcgt tcgacgtaga tgatgcagtt gatgcgcgtg aaatcttcgc
      781 gctcctcgtc gaagcgttcc gtcaggactg ccgtgacgta agggatttcg tcgcccgtcg
      841 tctgcaaaat cttttcgcgc acgatctccg cgcagagcgc gcgggttgat tggtcggtca
      901 attcgtcttc ggcgaagagc ggttcctgtt cgggcaggtg cttgacgatt ccggcgagca
      961 gcgtgtcaac catgtcgccc gtgcgcgccg agagcggcac gatttcgcgc cagtcgtact
     1021 cgcccgcgta ccactgcatg agcggcagga gcttggcctt gtcgcccacg cggtcaacct
     1081 tgttcagcag aagcagcgcg ggcttgcccg actctttgac gagatcgagc acgaagcggt
     1141 cgccgttgcc ggtgggcacg ctggcgtcgc gcatcaggag cacgaggtca acgccttcga
     1201 gtgcgtcgtg cacggcggcc atcatgcgcc ggttcaggag gtagccgggc tggtgcacgc
     1261 cgggcgtatc aaccagaacg atctgccctt cggggcgcgt gatgatgccc tgaatctggt
     1321 agcgcgtcgt ctgcggcttg ttggacacgg cggcgatctt ttcgccgacg agacgattta
     1381 agagcgttga cttgcccgcg ttcggtcgcc cgatgagcgc gacgtagccg ctcctgtaat
     1441 ttgcttgttc agacataagg aaaaacttta atcgctgctt tagctcctga caaagagaga
     1501 agcgagtgac gagttgaaaa cttaagacgt gtctttcatc tcgtcacttg tcacctgtta
     1561 ctcgtcactg ctttatttgt gccgagcgcg attatcgcac gacgcaaacg cgcatgacga
     1621 cagagcagcc gcaaataacg ccgctcgtcc gcgcccgcct tcgcacggat gaccgcaagc
     1681 gcgtcgcgtt tttcctccct cgtccagcgc gcgagatcgg ggaccagcgc gagcaccagc
     1741 gcgaagtttg agaaggcgcg ttcctccgct tcattgaaag gttcacgatt cgcgccgagc
     1801 gcgcgcccga ccgctttgac ggacgcgtcg cgaatcgcct gcgcctcgcc gtcgagttcc
     1861 cgcgccattc tgcggttgat cgccagaccg atgtttcgca cctgaaagct gtcccacgcg
     1921 cccggcggcg gcgtcgccgt agcgtcgcgc gtctcgaaca acatgtggcc ggaggcgaga
     1981 cgacggagca ggcgcggagg cgtgcgatag tctctgcgcg cggcgagcct gcgttcttcc
     2041 gtcgccaaca gtttcgccaa ctcctcgcgc gtgggacgaa agcccagctt gcgatagaac
     2101 cagaacgcgc ccgacgcgat gccttcctcg ttgcccgacc cgccgagttg atacgggtct
     2161 atcgaaaaag tgtcgacgcc cgacacctgc ttgtagagcc gcatcaggcg cgcgtaagcc
     2221 cacgccgatt cgccgtcgcg aaacgtgtag aacaggttga ggccgatctc cacgcgctcg
     2281 aagagcgaca gcccttcgtg ataacccatc ggcacgccgt tcttgaaaaa tatcccgcag
     2341 tgataggcga gcagcggcag gcggcgttcg ggacgcacgc cccacaggaa aatctcaagg
     2401 ccgcgccccg cctctgcgcg caggacgcgc tccgggtcgc catgcgtgaa gccgtgcagt
     2461 tcacgaaaac ggacggccat cgtgtcgcgc ccggatgcca gcagcctttc gccttcggcg
     2521 cgcgacagac gctcgacgcg cagcctcctc cccggcgcgt ccaactcatg cgccagagag
     2581 acttcgcggc gaccgagcaa gggagcgtcg tgataaaaaa tttcgcgcgc ccgtcgtctc
     2641 cgccgcatga gcgtgcgcgt ggctttcgac ttgccgagtt cccagcgcac ccacagcttg
     2701 agcggctcga acagttccgc acgcgccttg tcgccggtgg gcagacgctc gaacgccctg
     2761 acgagccaag cgagatcgct ctcgcccttg cgcttggcgg cgcgcagcca tgcgagagcg
     2821 ggcgcgtgcg cgtccacgta agcgtcctct tcaaagagcg gcaacaatct cggccacacg
     2881 aggttcaagt gcgcgtggtc tgcttcttcg tcccagacga gcgagacgcg ccgagggtgc
     2941 gtgcgtgcca gccagcgcgt gatgtcgtag ccgaagacgg ctgtgaaccc ggtcgcggcg
     3001 atgcctgaca cgtccggctg ctcgaagggc gtgaggtctg cgcccgaagc gcgcaggcgt
     3061 tcgacgcgcg ccgcgaatga atcgagaatc ttctccgtgt gtgcgaggag cgacgcgctc
     3121 tgcggatacg cgcgcaggta gagcgtaatt tcgtgaaagc gaatgagcgt ctccgcctcc
     3181 gtgaaactgc gtcgcgtcgc ctgtctgagc acacgctcaa gacgcgcacg ctcgcgcggg
     3241 tcgccgccgg gacgcttcaa ctcgtcgagt cggtcgagca gttgttcgat tgatggtgcg
     3301 ggcatgcgcg attcggagcg tgaacaattt agacgagcgg gaaccttcta gcgtaagcct
     3361 ccgggcaagt caacgcgctt cgcatcccgc ttcgacttac agaggccgac gggtgttgag
     3421 ttccgccgtc tgcatcttta gtgaagaacg aaacgcgccg cagtctgttg acaggcggcg
     3481 cgttagtttg ttgacaggcg gcgcgttgtt gggatgacga tgacgcgcct tcgcggcaca
     3541 ggcggctacg gcctggagac tatttctgcg ccatctcgcc gttgactttc aggacttcga
     3601 gcgtcatctc ggcggaggcg tagaggtctt tcacgtcgag ccattctttg acggtgtgaa
     3661 tggcgcgcat gcctgtgccg aagttggcgc attcgatacc gcgcttgttg aagatgttgg
     3721 cgtcgcagcc gccgccggaa gccagcgtct caactttctg gccgaggcgg gcggcggcct
     3781 tgtagacgag ttggacgacg cgcgaactgt ccggcacgtc catcccggag tattcgcgcg
     3841 tgacgtgcga ctcgacgcgc cccttcgtcg tcacgcccgc caccgtcact tcccacttcg
     3901 ccgccgcgtc ttccagacac ttgatcatgt gcgcggtctg cgcttcgagt ttcttgtcgt
     3961 cgagcgagcg cgcttcgccg cgcagcagca cgaagttggt cgtgatgttc gtggccttgc
     4021 cgccttcgat gacgccgatg ttggcggtcg tctcgtggtc gatgcgcccg agtttcatgt
     4081 tgtgaatggc ttcggcggcg atgcggatgg cggagatgcc ctcttccggc gcgactcccg
     4141 cgtgcgattc gaggccgtag attttgaaat ccatgtgatc ggcagagggg cctttggtga
     4201 agaggaagcc ggggtcgtcc gagtcgaaga cgaggccgta tttcgctttc aacttcgacg
     4261 tgtccacgtg ctttgcgccg agcagtccga cctcttcgca gatcgagaag acggcttcga
     4321 taggcgcgtg cgggatgttc tgttcttgca ggcagcggat ggtttcgagg atgacggcgc
     4381 agccggattt gtcgtcgccg ccgagcacgg tcgtgccgtc ggaacggatg atgtcgccct
     4441 cgacgatggg cttcacgccc tcgccgggca cgaccgtatc catgtgcgcg ctcatcatga
     4501 tcgtctcggc gtccgggatc gtaccgggga agcgcgcgat gacgttgccg gagttgccgc
     4561 cgaccttctc tccggcgtcg tctatttcca ccgtcgcgcc catctcttcg caatattttt
     4621 tgatgcgctc ggccacgtcg cgctccttgc gcgagacgct gtcaatctgg acgagttcga
     4681 gcagtaagtt tttaatgcgc tcctggttga tcatttctcc tccgttatcc cggcaagcat
     4741 aaattgtaga aaggcttggc tcaatcctac gatacgccgc gttttgtctg caatatcaac
     4801 gatgcctgac gttgagggaa aattagtcgt tcagcgcgtc gagcgcgcgt tggagttcgt
     4861 cgcgcttggc aaagagtgcg gcttcgtcgg cgtctgcgga gacgtggatc aggggggcga
     4921 gacggacgcg cgcgcgtgtg aagggatagg gaatctggag tgcgtcccag gtcggcgcgg
     4981 cgtaatagcg tgcggcgtct acagtgaagg ggagaatggg cgcgccggtt tttttggcta
     5041 agagcactgc gcccattttg gcgacgtagc ggggaccacg cggcccgtcg atggtgaacc
     5101 cgccggggcg gccttgccgc atcagtttga tcatctcgat gagcgcgccg accgcgccgc
     5161 gcgaacttga gccgcgcgct gcgccgtagc cgaagcgttg aatgaagcgc gcgatgtatt
     5221 cgccgtcgaa acttcttgag gtcatcacga cgatgccgcg ccgccggaag aagtaggtgc
     5281 cgagaaagac gcggttgtgc cagaaggtgt agatcggctg cttccctccg cgcgtcgcag
     5341 cctcccaatg ctcccagcct tcgacctcga aacgcgcggt gcgcccgacg aggttgatga
     5401 gcacgtagaa ggcgaggtcg gcggcgcgga tgacgaagcg ttgcctgaat gagtaagcgg
     5461 agagcgaggc gaagcggtcg gcgcgcgccg gaggactcgg ctcgtcggcg tgcgtcggag
     5521 tcttctcaag gcggcgcgaa actgtctgcc cgccgtcggg cgcatgcgtt tcgcggcgcg
     5581 cggggggcga ctcgccttcg ggttcttcgc ctttgtcgag agagacttgc atgggataaa
     5641 ccgcgcgagt ctagcagaaa tgcgggttgt tttcgattta agggcacggg gcgcgtgcgc
     5701 ggttgccgct ttcgcggctg ctttcaggtt ggggcgtgtg ctatattcag gaaacctcgt
     5761 gccgttttat ggcgtactcg taaaaatgga agcgtgtctc cctcaggcgc gctttttccg
     5821 tgcctcgccc cgctctgtaa ggagtctgaa gtggaatcgg tgattgatgg ataaacgtcg
     5881 aatcctgatc gtggatgcca acaaggaact gcgcacgctc ctgtcgcagg tgcttggcga
     5941 gttcggccac gaagtagtgg ctacgggcaa ccgcgacgat gcgctcgccc gcgaagacct
     6001 cgaagacttc gatctcatca tcagcgattt gacggaagac gccgaaggca acgtgcaggt
     6061 cttgagcgag atcaagcgcc gcaggctcgt cgtgcccgtc gtcgtctcct cggaagactc
     6121 gcagcatccc ggcctcgtca aagccttcaa gatgggcgcg gccaactacc tgcgccgccc
     6181 ttacgacaaa caggaactcc aagccatcgt cgaaaaaaca ctcggctaca agctgcgctt
     6241 cgtcgaagac atgaaggtcg tgccgttcgt gcgcgagcgg atcgagttcg agttgccgag
     6301 cgatctctct ttgatgaacg gcgtcctgca ttacctgcac gagcgcgcgt cgaaactcgg
     6361 cctcatcaat cccgaacgct ccaatctctt catcgcgctc gacgaggctt tcgtcaacgc
     6421 cgtcaagcac ggcaacaagt atgatcaaaa gaaactcgtg cgcatcaccg ccgatctctc
     6481 cccgaaggaa gcgcgcttca ccatcgaaga cgaaggcgag ggcttcaacg tcaaagatgt
     6541 gccagacccg cgcgacccgg ccaacctgtt caggacttcg gggcgcggcg tcctcctgat
     6601 ttacaacatt atggacgagg tggcgtacaa tgagcgcggc aatcgcctga cgatggtgaa
     6661 acgtcccgaa gaatcgttcg accataaact gattgactcg ctcactcccg aagaccacga
     6721 ccgccgccga cgcacgcgcc gccgcccgac ctctaacagc ggatgatgtt tcgctcgcgc
     6781 cgcccgctgc cgccagcgtc gtcgcgacgg cgacacacgt tgacgacagc cgcgacagcg
     6841 gcgcgctccc gacgcgacag gcgtggcgtt ggtggcttcc gcccacgctc tccgcgctcc
     6901 tcttcaccat ctattttata gacccgttcg ccggtgactg ggacgcgctc gactacacgg
     6961 ttcatgccgt gcgcggcgag ccgtcttcca tgctcttcgg gcgcatgctc ttcatcttca
     7021 taaaccacgc ggcctatcgc gtggcacacg cgctcttcgg attgccgtcc gagcaagcct
     7081 acctgctctt caaatacatg gttgtcgtgc agacgccgtt tgccgtcgtc gcctgctgga
     7141 cgctcgcgcg tgagttgtcg ggttcgctgc ggacggccac ggtcgccgct ttcatgctcg
     7201 cgctctcgcc cttcttcatc gtctatagcg ggcaggcgat gacggagacg ccttcgatcc
     7261 tgctgctctc gattgcgctc atcacgcacc tgcgcggcgt gcgcgctcgc gtgaactggc
     7321 aggtgctcgc gggcgcggcg ttgctcggcc tgtgtgtgaa catacgcgag ccggtcggct
     7381 tcttcggcgt gtggctcgtc ctcgcgccgc actgttgtta cgggtggaga ctacagcggc
     7441 gcgaactatt tttgaccgcg ctggcctgcg ccctcttctt aacctgcgcg ctcgcgccct
     7501 tcgccctctg gtggctgctc gacgtgcgcg gctatcgcgc aaactggtac gggtggctcg
     7561 cttcgatgcg cgcggagtcg gcggtgcatc cggtcaaact ctcgaacctc gtgcccttcc
     7621 tccaactctt cttcatcgcc gccccggcca cgttcctcgc gctgccgttc gcactgcaac
     7681 gcgaatggcg cgaggggcgc aaggcgacgc cgatcttcgt gatggcgctc gtcggcctgt
     7741 tcgtccaact gatgctgctg gcaaactaca gcaccgtgct caacgggcga taccagttga
     7801 cgggactgcc cggactcttt cccctcaccg ccgcttacct gcttcgtcgc acgtcggagc
     7861 gcacgcgccg ccgtcgtagt agtaccagta gtaacagccg cagcccgcgt cgagctttag
     7921 tctatacttg cgcggtcgtc gcctgcgcct cgctcctgac cgga
//
LOCUS       DASWNS010000317         1937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_90359, whole genome shotgun sequence.
ACCESSION   DASWNS010000317 DASWNS010000000
VERSION     DASWNS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90359"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1937)
                     /locus_tag="VGB76_11080"
     CDS             complement(<1..>1937)
                     /locus_tag="VGB76_11080"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY0099481.1"
                     /translation="SQMMTRIVKVQLVDKENQAQANARTLAEAFLQRVPQNGDTRAAQ
                     AWLDERVELLQPSLPGARVAVWSGNGAVDGSKPAQYMSEPRDERVRGVGNDQTPIGAP
                     LPAWLRAREEWHGFAYLPPPPSSEEAFGTPSMRAFVRARAGEREVLLLFVLPVSRVFV
                     EQLRLNTDLQVQPFFIGAEMAAVRFTQGWTGSSISLGPPRARRMSREDELKLEIVEGG
                     RRVVIDFSKDQLGDPVDMSSMIVLPATSWTDGAVSQRAAFLCKFSAFAMANQLFQESD
                     LGRYFQGAFILVAVVFLLLELFALLSAAWLTRAVTGTVHKLHRATGFIKRGDFSHRVR
                     VRSRDQLGELAEAFNEMSANIESLLQERVKHERLEREVEIAAEVQAQLFPRKVPHLSA
                     AEIVGECRAARGVAGDYYDYVEIAPGLVAFALGDVAGKGVSAALVMSNLQAALRAQAT
                     ILAERMRTLSARAAATTAIGGSGASGQNVEMPCGVTGVDIECAVEQMAASINEQLCHS
                     TDSNRFATLFIALYDDATRQLRYTNAGHNDALLIRAGSGTVEHLTEGGTVLGAFEWAR
                     YTEARTTLERGDVLVIYSDGISEADNPRGAEYTEHRLAHLVRKNRDKSADELRQLIFT
                     EVDNWTGGQERDDDQTVVILK"
BASE COUNT          322 a          655 c          617 g          343 t
ORIGIN      
        1 tcttgagaat gacaaccgtt tgatcgtcgt cgcgttcctg cccgcccgtc cagttgtcca
       61 cttccgtgaa gatcagttgg cgcagttcgt cggcgctctt gtcacggttt ttgcgtacga
      121 ggtgcgcgag gcgatgttcg gtgtactccg cgccgcgcgg gttgtcggct tcgctgatgc
      181 cgtcggaata gatgacgagc acgtcgccgc gttcgagcgt ggtgcgcgcc tcggtgtagc
      241 gcgcccactc gaacgcgccg agcactgtcc cgccctctgt gagatgttcg accgtgccgc
      301 tcccggcgcg gatgaggagc gcatcgttgt gccccgcgtt ggtgtagcga agctgccgcg
      361 tcgcgtcgtc gtagagcgcg atgaagagcg tggcgaaacg gttcgagtcg gtgctgtggc
      421 agagttgctc gttgatgctc gcggccatct gctcgacggc gcactcaata tcgacgcccg
      481 tgacgccgca gggcatttcc acattctgcc cgctcgcgcc gctgccgccg atggccgtcg
      541 tcgccgccgc gcgcgccgac agcgtgcgca tgcgctctgc gagaatggtg gcctgtgcgc
      601 gcaacgcggc ctgtaagttc gacatgacga gcgcggcgga gacgcctttg cccgccacgt
      661 cgccgagcgc gaaggcgacc agaccgggcg cgatctccac gtagtcgtaa taatcgcccg
      721 ccacgccgcg cgccgcgcga cactcgccca cgatctccgc cgcgctcaag tgcggcacct
      781 tgcgcggaaa gagttgcgcc tgcacttccg ccgcgatctc tacctcacgc tccaacctct
      841 cgtgcttgac gcgctcttgc agcaacgact cgatattggc cgacatctcg ttgaaggctt
      901 cggccagttc gccgagttgg tcgcgcgagc ggacgcggac gcgatgcgag aagtcgccgc
      961 gcttgatgaa cccggtcgcg cggtgaagtt tatgtacggt gcccgtgacg gcgcgcgtca
     1021 gccacgcggc ggacaagagc gcgaacagtt cgaggagcag aaagacgacg gcgacaagga
     1081 tgaacgcgcc ttgaaagtag cgaccgagat cactctcctg aaagagttga ttcgccatag
     1141 cgaaggccga gaacttacag aggaaggccg cgcgttgcga caccgcgccg tccgtccaac
     1201 tagtcgccgg aagcacgatc atcgaactca tatcgaccgg atcgcccaac tggtctttgc
     1261 tgaaatcgat gaccaccctg cgcccgcctt cgacgatctc caatttcagt tcgtcctcgc
     1321 ggctcatcct gcgcgcacgc ggggggccga gcgagatgga gcttcccgtc cacccttgcg
     1381 tgaaccgcac ggcggccatc tccgcgccga taaagaacgg ctgcacctgc aaatccgtat
     1441 tcaggcgcaa ctgctcgacg aagacacggc tcaccggcag gacgaagagc agcaacacct
     1501 cccgctcgcc cgcgcgcgcg cgcacgaacg cacgcatcga gggcgtgccg aaggcttcct
     1561 cggaagaagg cggcggcggc aggtaggcga aaccgtgcca ctcttcgcgc gcgcgcagcc
     1621 acgcgggcag cggcgcgccg attggcgtct ggtcattgcc gacgccgcgc acgcgttcat
     1681 cgcgcggctc gctcatgtac tgcgccggtt tactgccgtc aaccgcgccg tttccgctcc
     1741 acacggcgac acgcgcgccc ggcagcgacg gttgcagcag ttcgacacgt tcatccagcc
     1801 aggcctgcgc ggcgcgcgtg tcgccgttct gcggcacgcg ctggaggaaa gcctctgcga
     1861 gcgtgcgcgc gttggcctgc gcctgattct ccttgtccac gagttgcact ttgacgatgc
     1921 gcgtcatcat ctggctc
//
LOCUS       DASWNS010000318         7897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_117237, whole genome shotgun sequence.
ACCESSION   DASWNS010000318 DASWNS010000000
VERSION     DASWNS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117237"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..497
                     /locus_tag="VGB76_11085"
     CDS             246..497
                     /locus_tag="VGB76_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0099482.1"
                     /translation="MPEQAEIETKVKQIIVDELGVDENEVTPNARFIDDLGADSLDTV
                     ELVMRFEEEFGIEIPDEDAEKIQSVRDAYNYIDQHLNKA"
     gene            664..1920
                     /gene="fabF"
                     /locus_tag="VGB76_11090"
     CDS             664..1920
                     /gene="fabF"
                     /locus_tag="VGB76_11090"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY0099483.1"
                     /translation="MKRRVVVTGLGLVTPVGLTVESTWAALLAGRSGVGYIEKFDTTK
                     FSVKIAAEIKEFDPLKFVEKKEARKMGAFIHYAIAASDEAIYDSGLIERGQKFAPDVA
                     ERVGTYISSGIGDFWAIEREHSKLLDDGPGRVSPFFIPSAIVNLAAGQVSIRSGAKGP
                     NSATATACAAGAHAIGDSFRIIERGDADVMICGGAESAITPMSVAGFAAMRALSTHNA
                     DPAHASRPFERDRDGFIIGEGAGIMILEELEFAKARGAKIYAEIAGYGATADAFHITM
                     PDETASGAIRVMQMALRDGGAQPEQVDYINAHGTSTPYNDKFETLAIRKTFGEHADRL
                     AVSSTKSMTGHLLGAAGGIEAVFSVLSITRNMLPPTINYVNPDPECDLDYVPNEAREV
                     RVNYALSNSFGFGGTNAALLFKRYEE"
     gene            2004..2780
                     /locus_tag="VGB76_11095"
     CDS             2004..2780
                     /locus_tag="VGB76_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY0099484.1"
                     /translation="MKIIVLMKQVANKDAILRINKSETWIEEGDLSYEVNESDGYALE
                     EALRLKEKHGGEVVVCSMGPQRVKSVIKDALARGADRALHIVSDDGGAQLGPYAAAKV
                     LSAAIKDEGADLILTGLQSDDNGYAQTGVILAELLGLPHATIVIEVDASNEGLRVKRE
                     LESGWYQWYAMSRPALLTIQSGISQIRYATLKGIMAAKKKEIKEIAPAADAASSAAHQ
                     RVTKVYFPQKTKQTQFLGDGDAKAGAAQLAEKLRTEARVF"
     gene            2869..3855
                     /locus_tag="VGB76_11100"
     CDS             2869..3855
                     /locus_tag="VGB76_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY0099485.1"
                     /translation="MPNGILVFAEHRGGAFNKTSFEAIAAAQQLGASLGQSVTAVVLG
                     TDATLAGEVAAYKLDKVVSVENPKLADYTPDAYADAMERAVRALDPQFVIMPHTYLVR
                     DYAPKLAARFGKDLISDCVRATVADNAVTFSRRIFQGKIDADVVSNDEPPVFATFQSG
                     AYRADQAERGGDGAPAAVESLQVEVGEVRMQPETPFQEVKQAVDLTKADTIIAIGRGI
                     KGQEHIALAEQLAAALGGEIAASRPICDAGWLPIDRQIGSSGQTVTPKLYIALGISGA
                     IQHLVGMKNASTIVAVNKDPEAPIFDIADYGIVGDLFEVLPVLTEEIKKLKG"
     gene            complement(3934..4845)
                     /locus_tag="VGB76_11105"
     CDS             complement(3934..4845)
                     /locus_tag="VGB76_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0099486.1"
                     /translation="MKKISDDRRRAQWLLPILLTGIVFAACSPPPPDPNRGQRYELKG
                     KIVSFNKAQQQVVIEHEAIPDFMEAMTMPFTLKEEAAYDVMRAGDTIQATLVVDAERT
                     RLENPIITQAIPGESKAGESSDGALLREPEIGASVPDVSLVNQDGKPINLQQYRGRAL
                     VITFIYTRCPLPDYCTLMSNNFAAIRRELDKSPELRAGAHLLSITLDPAYDTPKVLRS
                     YGAAHTENYGDEKFARWEFATGEPQAIQRTANFFGMAYKQEGDEIVHALRTAVIAPDG
                     KLYKMYRGNEWKPEEVVQDLRDLQSRK"
     gene            5339..5767
                     /locus_tag="VGB76_11110"
     CDS             5339..5767
                     /locus_tag="VGB76_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0099487.1"
                     /translation="MQQIRILYAEDYDLVLFTVKQLLELEGWQVDICRDGLAALKKLE
                     SDDHYDLIILDAQMPGESGLALVQRARQLAHRRRVPVIMFTGSLRREEALAAGADAFL
                     NKPTGLKDLIATCNSLLDKRVISERETSDDLQKSVGNLHR"
     gene            6079..7023
                     /locus_tag="VGB76_11115"
     CDS             6079..7023
                     /locus_tag="VGB76_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEY0099488.1"
                     /translation="MNEEKRRARILDGARVAEEIKREVAGDVARLKEMHDYAPCLVAV
                     RVGNDPASAVYVRNKVRTSEALGLRSEHHELPEETTLEQLLAEIKTLNARDDVDGILV
                     QLPLPRGIDETRVLEAIDPAKDVDGFHPINVGRLTLGRTGFAPCTPAGIIELLTREKI
                     ELRGQHACIVGRSHIVGRPLAQLLLQHDATVTICHSRTRELPAHTRQADLLIAAVGQT
                     GLIRAEHVKPGATVIDVGMNKITDAQTARDFFGENSASRIEAINKRGFTLVGDVHPAE
                     VSRVAGQLTPVPGGVGPLTVAMLMRNTLTAAERRRGLS"
     gene            7028..7639
                     /gene="coaE"
                     /locus_tag="VGB76_11120"
     CDS             7028..7639
                     /gene="coaE"
                     /locus_tag="VGB76_11120"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY0099489.1"
                     /translation="MLRAGLTGSIAVGKSFVSSVFAESGCYVLDADRTAREVVAAGSP
                     GLRAVADAFGSEVLQADGELDRAKLGAIVFGDETKRARLNEILHPLIIAAQNDWLRQR
                     EREDARGIGIVDAALMIETGSYKRFDKLIVVHCRAELQLERLVKRDGLTVAEAVRRIE
                     AQMPQEEKKKFADYLIDTSNGFEDARAQASAVYEKLKALATQS"
     gene            7666..>7897
                     /locus_tag="VGB76_11125"
     CDS             7666..>7897
                     /locus_tag="VGB76_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099490.1"
                     /translation="MMRKRGRKIFPGLKLSRWRAPLAVCLAVTLTIASGGCFSSDEGE
                     LYYGSVRVPSRQEFRWSDGGLPQIFDPALAAAP"
BASE COUNT         1657 a         2254 c         2433 g         1553 t
ORIGIN      
        1 agggcgtgcg gacgttcgta gaggtcggcc ccggcaaagt tctctccgga ttagtgcggc
       61 agacgatcac ggacgcgcgc tgtctgaacg ttgcagaccc ggccagcctc gcagccacgc
      121 gcgcggcgat ggccgaaacg caggccgcga gcggtggcaa ttaacgtgcg ggcgtgtttt
      181 aatttgcccc taacacatag accaagtttt tctatttttt aattgaccga aggagcagaa
      241 ctagtatgcc cgagcaagca gagatcgaga ctaaagtgaa acaaatcatt gtggacgaac
      301 ttggcgtgga cgaaaatgaa gtgacgccca acgcgcgctt catagacgat ctgggagccg
      361 actcgctcga caccgtcgaa ctcgtcatgc gtttcgaaga agagttcggc atcgagattc
      421 ccgatgagga cgcggagaaa attcagagcg tgcgcgacgc ctacaactat atcgatcagc
      481 acttgaacaa ggcgtaagac agtcgccgcg agttggcgtg ccgcttggcc gtcgctcgtt
      541 cgccgctgtt tcagatcggt cggaaatttt ctgttgcccg gcgatgttcg cccgcgcgcc
      601 aaaagcgcgt gttgctggca ggggcaacgg cacgagcaat ccctcggagg gaggagcagt
      661 aatttgaaac gtcgcgtcgt cgtcacggga ctcggcctcg tcactcccgt cggtttgaca
      721 gttgaatcca cttgggctgc cctgttggcg gggcgcagcg gcgtgggcta tatcgagaag
      781 tttgatacga caaagttctc cgtcaagatc gccgccgaga tcaaggagtt cgacccgctc
      841 aagtttgtcg agaagaaaga agcgcgcaag atgggcgcgt tcattcatta cgccatcgcc
      901 gcctccgacg aggcgatcta cgatagcggc ctcatcgagc gcgggcagaa gtttgcgccc
      961 gacgtggccg agcgcgtcgg cacctacatc agcagcggca tcggcgactt ctgggctatc
     1021 gaacgcgagc actcgaaact gctggacgac ggacccggtc gcgtctcgcc cttcttcatc
     1081 ccttcggcca tcgtcaatct ggccgccggt caagtctcca tccgcagcgg cgcgaaaggt
     1141 cccaactcgg ccacggccac ggcctgcgcc gccggggcgc atgccatcgg cgactctttc
     1201 cgcatcatcg aacgcggcga cgccgacgtg atgatctgcg gcggtgcgga gtcggccatc
     1261 acgccgatga gcgtggccgg attcgccgcc atgcgcgcgc tctcgacgca caacgcagac
     1321 cccgcgcacg cctcgcgccc gtttgaacgc gaccgcgacg gtttcatcat cggcgagggt
     1381 gcggggatca tgatcctcga agaattggag tttgccaaag cacgcggcgc gaagatttat
     1441 gcggagatcg ccggctacgg cgcgaccgcc gatgcgtttc atatcacaat gcccgacgaa
     1501 accgcttcgg gcgcgatccg cgtcatgcag atggctctgc gcgacggcgg agcgcagccc
     1561 gaacaggtgg actacatcaa cgcgcacggc acttcgactc cctacaacga caagtttgag
     1621 acgctcgcca tcaggaagac tttcggcgaa catgccgacc gtctcgcggt cagttcgacg
     1681 aaatccatga cggggcacct gctcggcgcg gcgggcggca tcgaggcggt cttctcggtg
     1741 ctctccatca cgcgcaacat gcttccgccg acgatcaact acgtcaaccc cgatcccgaa
     1801 tgcgatctcg attacgtgcc gaacgaagcg cgcgaggtgc gcgtcaacta cgcgctctcg
     1861 aacagtttcg gcttcggcgg cacgaacgcc gcgctgcttt ttaaacgtta cgaggaatga
     1921 aggagccgtt agctgtcagc gaatagcggt tagccttttt cttccggcta acagctcacc
     1981 agctaacggc tcataactaa cccatgaaaa tcattgtgct catgaagcag gtcgccaaca
     2041 aggatgcgat cctgcgcatc aacaaaagcg agacgtggat cgaagagggc gacctgtcct
     2101 atgaggtcaa cgaatcggac ggctacgcgc tcgaagaagc cctgcgcctg aaagagaaac
     2161 acggcggcga agtcgtcgtc tgttcgatgg ggccgcagcg cgtcaagagc gtcatcaagg
     2221 atgcgctcgc acgcggcgca gaccgcgcgc tccacatcgt ctccgacgac ggcggcgcgc
     2281 aactcggtcc ttatgcggcg gccaaagtct tgtccgcagc gattaaagac gagggggctg
     2341 atctcatcct cacgggtttg caatccgacg acaacggcta cgcgcagacg ggcgtgatcc
     2401 tcgcggaact gctcgggctg ccgcacgcga ccatcgtgat cgaggttgac gcgtcgaatg
     2461 aagggttgcg tgtgaagagg gaacttgaga gcggctggta ccagtggtac gcgatgtcac
     2521 gtcccgcgct cttaacgatt caatcgggca tcagccagat tcgctacgcg actttgaagg
     2581 gcatcatggc ggcgaagaag aaggagatca aagagatcgc gcccgccgcc gatgccgcgt
     2641 cttcagccgc gcaccagcgt gtcacgaaag tttactttcc gcaaaagacc aagcagacgc
     2701 agtttctcgg cgacggcgac gccaaagccg gagccgctca actcgcagag aagttgcgca
     2761 cggaagccag agtattttaa gagccagtag ctttcagcta tcagctgtca gcttagaaat
     2821 cgctgaaccg ctgaaagctg acggctgata gctgataact aaatttatat gccaaatgga
     2881 attttagtct tcgcagagca ccggggtggt gcgttcaaca aaacttcctt cgaagccatc
     2941 gccgccgcgc agcaactcgg cgcgtcgctc ggtcaaagcg tcacggcggt cgtgctcggc
     3001 acggatgcga cgctggcggg agaagtcgct gcgtacaaac tggacaaagt cgtctcggtt
     3061 gagaacccga agttggccga ctacacgccg gacgcctatg ccgacgcgat ggagcgagcc
     3121 gtgcgcgcgc tcgacccgca gttcgtcatc atgccgcaca cttatctcgt gcgcgattac
     3181 gcgccgaaac tcgcggcgcg cttcgggaag gatttgatca gcgattgcgt gcgcgccacg
     3241 gtcgccgaca atgcggtcac gttctcgcgc cgcatctttc aagggaagat tgacgccgac
     3301 gtggtttcga acgacgagcc gcccgtgttt gcgacgtttc agtcgggcgc gtaccgcgcg
     3361 gaccaggcgg agcgcggcgg cgacggggca ccggcggcgg tcgagtcttt gcaagtcgaa
     3421 gtgggcgaag tgaggatgca gccggagacg cctttccagg aggtgaagca ggcggttgac
     3481 ctgacgaaag ccgacaccat catcgccatc gggcgcggca tcaaagggca ggaacacatc
     3541 gcgctcgcgg agcaactggc cgccgcgctc ggcggtgaga tcgccgcttc gcgtccaatc
     3601 tgtgatgccg gttggctgcc gattgatcga cagatcggtt cttccggtca gaccgtcacg
     3661 ccgaaactct acatcgcgct cggcatctcc ggcgcgatcc agcatctcgt cggcatgaag
     3721 aacgcgagca ccatcgtcgc cgtcaacaaa gaccccgaag cccccatctt cgacatcgcc
     3781 gactacggca tcgtcggcga cctctttgaa gtgctcccgg tcttgaccga agagatcaag
     3841 aaactcaagg gctaggccgc ggcaaagcaa actccataag atcgagacgg gggcggcgtg
     3901 tgataaacgc gccgcctctc tttttcgagc cgctcactta cggctttgca ggtcgcgcaa
     3961 gtcctgcaca acttcctcag gcttccactc gttcccgcga tacattttgt aaagtttgcc
     4021 gtcgggcgca atgacggcgg tcctgagagc gtggacgatc tcatcgccct cctgcttgta
     4081 ggccatgccg aaaaaattgg ccgtgcgttg aatcgcctgt ggctcgcccg tggcaaattc
     4141 ccagcgcgca aacttctcgt cgccgtagtt ttccgtgtgg gctgcgccgt aactgcgcaa
     4201 gaccttcggc gtgtcgtacg ccggatcgag cgtgatgctg agaagatgcg cgcccgccct
     4261 caactccggc gacttgtcca actcgcgcct gatcgctgcg aagttgttgc tcatcagggt
     4321 gcaataatcg ggcagggggc agcgcgtgta gatgaaagtg atgacgagcg cgcgcccgcg
     4381 atattgttgc aggttgatcg gtttgccgtc ctgattgacg agcgacacgt cggggacgct
     4441 cgcgccgatt tcgggttcgc gcagtagcgc tccgtccgaa gactctccgg ccttgctctc
     4501 tccggggata gcctgcgtga tgatcggatt ttcgagacgc gtgcgttcgg cgtcaaccac
     4561 gagcgtcgcc tgtatcgtgt cgcccgcgcg catgacatcg taggccgcct cttccttgag
     4621 cgtgaagggc atcgtcatcg cttccatgaa atcggggatg gcttcgtgct cgatcaccac
     4681 ctgttgctgc gccttgttga aagagacgat ctttcctttc aactcataac gctggcctcg
     4741 gttcgggtcg ggcggcgggg gcgagcaggc tgcgaaaact ataccggtta acaggatcgg
     4801 gagtaaccat tgagcgcggc gtctatcgtc tgaaatcttc ttcatgggta aagtttatca
     4861 acaattatcc tcgaacaatt tttagaatta cacgctatgt atagcattta agcgaagccg
     4921 ctcccaatcg cgcgggcgga cgagatggca gaccgcgtac gcttcgtgca gtcttttgtc
     4981 gatttaaccg tgtggcaaaa tcgaaacacg actgcaagta acctcgctaa gcgggttaaa
     5041 tcctgtgttt ccggttttta atgctccgca ggaaaatatc gccatgtaac tttttcaatt
     5101 cagagtcaac cgctgcctcg cgggcgcgcg ttggaaagca cctgtctccg tgcccgactt
     5161 atgggttatc gcctctatta cgtgcagtta gcgtttcccg gtgaagttgc cggtggaggt
     5221 gtcggaagcc ggggaacgat agttgccatg tgtgtctgcg aaacatgtgt tttcattcgg
     5281 ttgggcgcgc tcaagttctc cttaccaacc tcactattac aaaggaaggt gccttgattt
     5341 gcagcagatc agaatccttt atgccgagga ttacgattta gtcctcttta cggtcaagca
     5401 gttattagaa ctcgaagggt ggcaagttga tatttgtcgc gacggtttgg ccgcgctgaa
     5461 gaaactcgaa agtgacgatc actatgatct gatcattctc gatgcgcaga tgcccggaga
     5521 gagtggtttg gcgttggtac aaagggcgcg ccagttggca caccgccgcc gcgtcccggt
     5581 catcatgttc acgggtagcc tgcggcgcga agaggctctg gcagcaggcg cagacgcttt
     5641 cctgaataaa ccgaccggct tgaaagacct gatcgctacc tgcaattcgc tgctcgacaa
     5701 gcgcgtgatc agtgaaaggg agacgagcga cgacctgcaa aagtcggtcg gcaaccttca
     5761 ccgttagaaa gggacgcaca cgagcgtccg aagttttaga tgtccaaagt gccgtcctct
     5821 gtgcggagtt agacggtagg tttgatgctt taaatttgct acctcacccg cattaagtta
     5881 ggaaggccgc gttcggctta agggcttgcg aatagtctgc actattcgca agcctttggc
     5941 ttttttgcgt gagacaaaag agcgcgagca gaaaaattga tgaggcgtag gcgacgcgcg
     6001 acgcccacgc gaccgacgta aacgagtgcc gccgaaatat ggtaggatgc ctccatcttc
     6061 gcaacgctta tgttaactat gaatgaagag aaacggcgcg cacggattct cgacggagcg
     6121 cgcgtggccg aagagatcaa gcgcgaagtg gcaggcgacg tggcgcgctt gaaggagatg
     6181 cacgattacg cgccgtgcct cgtggcagtt cgggtcggga acgatccggc gtcagccgtc
     6241 tacgtgcgca acaaggtgcg aaccagcgaa gcgttggggc tgcgctccga gcatcacgag
     6301 cttcccgaag agacgacgct tgagcaactg ctcgcggaga ttaagactct caacgcgcgg
     6361 gatgatgtgg acggcatcct cgtgcaactg cctctgccgc gcggcataga cgaaacgcgc
     6421 gtgctcgaag cgattgatcc ggcaaaagac gttgacggtt ttcatccgat caacgtggga
     6481 cgtttaacgc tgggtcgcac ggggttcgca ccctgcacgc ccgccgggat catcgaattg
     6541 ctgacgcgcg aaaaaattga gctgcgcggc cagcatgcct gcatcgtcgg tcgaagccac
     6601 atcgtcggtc gcccgctcgc gcaacttttg ctgcaacacg acgccaccgt caccatctgt
     6661 cattcgcgca cacgcgaact gcccgcgcac acgaggcagg ccgaccttct catcgcagcc
     6721 gtggggcaga cgggcttgat tcgcgccgaa catgtgaagc ccggcgcgac cgtcatcgac
     6781 gtggggatga acaagatcac cgacgcacag acggcaagag attttttcgg agagaactcc
     6841 gcctcgcgca tcgaagcgat caataaacgc gggttcacgc tcgtgggcga cgtgcatccg
     6901 gcggaggtgt cgcgtgtggc gggtcaactg acgcctgtgc cgggcggcgt cggccccctg
     6961 acggtggcga tgctcatgcg aaacactctg acggcggcag agcggcggcg cgggctgtct
     7021 taaagagatg ttgcgcgccg gactgacagg atcaattgcg gtcgggaagt cgttcgtctc
     7081 atccgtgttc gcggagtcgg gctgttatgt gctcgacgca gaccgtacgg cgcgcgaagt
     7141 ggtggcggcg ggaagccccg gcttacgcgc cgtcgcagat gctttcggct cggaagtgct
     7201 acaggcggac ggcgagcttg atcgtgcgaa actcggtgcg atagtctttg gcgacgagac
     7261 gaagcgtgcg cgtttgaatg agattctgca tcccctgatt atcgcggcgc agaacgattg
     7321 gttgcggcag cgagagcggg aagacgcgcg cgggatcggc atcgtggatg ccgctttgat
     7381 gatcgagacg gggagttaca agcgttttga taaattgatc gtggttcatt gccgcgcgga
     7441 gttgcaactc gaacgtctcg tcaaacgcga tggcctgacg gtggcggaag ccgtgcggcg
     7501 catcgaggcg cagatgccgc aggaagagaa gaaaaagttt gcggattacc tgattgatac
     7561 atcaaacggt tttgaagatg cgagagcgca ggcgtcggcg gtctacgaaa agctgaaagc
     7621 attggcgacg cagagttgac gttttcgcac agacgcttgc aaaagttgat gcggaaaaga
     7681 ggacgcaaaa tatttcccgg tcttaaactg tcgcgctggc gcgcgccgct ggccgtgtgt
     7741 ctcgcagtga cgctgacgat tgcgagcggc gggtgctttt cgagcgacga gggcgaactc
     7801 tattacggga gcgtgcgcgt gccgtcgcgg caggagtttc gttggagcga cggcggcctg
     7861 ccgcaaattt tcgaccccgc gctcgcagcc gctccgc
//
LOCUS       DASWNS010000319         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_505782, whole genome shotgun sequence.
ACCESSION   DASWNS010000319 DASWNS010000000
VERSION     DASWNS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505782"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2973)
                     /locus_tag="VGB76_11130"
     CDS             complement(<1..>2973)
                     /locus_tag="VGB76_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099491.1"
                     /translation="GNALAHRWYAFNLAAIGRHDDSISEIKRALQIAPDSPVNNTALA
                     NVLFLARRFDEAIEQCKHALELDPGSVAAHVILRWAYEQKGMHEEALSTYEQESVFAG
                     DTPTTRLKEAHVLAAGGRRDEAVALLRELLARRDEQWVTSYEIAVIYSLLNERDEAFH
                     WLAQAENEHAVGFTFAGVDPHLDNLHADPRFAEILRRTNNPLAARYAGEAARREARQF
                     DPHLSSLPTLEFTTNGNKKPAEQEKPAPPPPERRLSRRAVGSALTVALVVLAAVTTGA
                     FFYAKRSNRSIAALAGASPLRLTTEIAHDWHPDYSPDGTRIAFASNRDGATEIYTMRA
                     DGSDVRRLTYNTTEDDCPAWSHDGTKIAFQSRRDGQMEVYVMDADGSNQRNLTNAPGE
                     DTRPSWSPDDRRIAYGSNTLDAPTNYDVFTMRADGSDKIKLTDDPRFDSDATWSPDGQ
                     KIAFTSARDGKSYEIYVMNADGSDVRNLTNHPGDDAKPAWSPDGKHITFAGNRPNKTD
                     YPAVYIMDADGNNQRLITGARTFDDEPGWSPDSRRVAFQTQRDGNFEVYLADAFPTTD
                     AGAANVGGAGKTRSLAVLPFTTVGAEGDEQYLGVGIADELTNKLGELDKITLRTASAL
                     RRYLGTDKSPVEVGRELKVEYVLAGWVERVGNRVQTSLELIDVESGRVLWAEKFNERF
                     TDMSTLQNSISARVVRALSLELTSDEQRRLSKHYTEDSEAQQLYLAGRYHWGKRTPEG
                     LRMAISNFEQAIAKDPRFALAYAGLADCYALQNWYLEPPPADAFARAKEAAERAVALD
                     DTLAEAHVSLAFVKFHYERDLQGAEAEFRRAINSNPNYATAHHWYAFNLSVMERHDEA
                     LLEIKRAEELDPRSAVISTAVANILFHARRYDEAIEQCRKALAIDPGSASAQIVLRWA
                     YEQKGMTDAAFAVYERERAFSGDTPTTRAKLAHVLAISGRKEEARRIVEELVARRKQQ
                     WVTAYEIAVVYAFLLDKD"
BASE COUNT          445 a          889 c         1037 g          602 t
ORIGIN      
        1 ttgtctttat cgaggaggaa ggcgtagacg acagcaatct cgtaggccgt cacccattgt
       61 tgtttgcgcc gggcgacgag ttcttccacg atgcggcggg cttcctcctt gcgcccgctg
      121 atggctaaga cgtgggcgag cttggcgcgc gtcgtcggcg tgtcgccgga gaaggcgcgc
      181 tccctttcat agacggcgaa ggccgcgtcc gtcatcccct tctgctcata cgcccaacgc
      241 aacacgatct gcgccgaagc cgaaccggga tcaatggcga gagctttgcg acactgttcg
      301 atagcttcgt cgtaccgccg cgcgtggaag agaatgttgg cgaccgccgt gctgatgacg
      361 gcggagcgcg ggtcgagttc ctccgcgcgc ttgatttcga gcaacgcctc gtcgtgtcgt
      421 tccatgacgg agaggttgaa ggcgtaccag tggtgcgcgg tcgcgtagtt cgggttagag
      481 ttgatggcgc ggcgaaactc cgcttctgcg ccttgcaggt cgcgttcgta gtgaaatttg
      541 acgaaggcga gcgagacgtg ggcttcggcc agcgtatcgt cgagcgcgac ggcgcgctcc
      601 gccgcttctt tagcccgcgc gaaagcgtct gcgggcggcg gttcgagata ccagttctgg
      661 agcgcgtagc aatcggccag acccgcgtag gcgagggcga agcgcgggtc tttggcgatg
      721 gcctgctcga aattcgagat cgccatgcgc aacccttcgg gcgtgcgctt gccccagtgg
      781 taacgcccgg cgagataaag ctgttgcgcc tcgctgtcct ccgtgtagtg cttcgagaga
      841 cgacgctgct cgtcgctcgt cagttcgaga ctgagcgcgc gcacgacacg cgcggagatc
      901 gagttttgca gcgttgacat gtcggtgaaa cgctcgttga acttctccgc ccagagcacg
      961 cgcccgcttt caacgtcaat gagttcgagc gaagtctgca cgcgattgcc cacgcgctcg
     1021 acccagcccg ccagcacgta ttcgaccttc agttcgcgcc ccacctcgac cggactctta
     1081 tccgttccca gataacgacg cagcgcgctc gccgtgcgca gagtgatctt gtccaactcg
     1141 ccgagcttat tcgtcaactc gtcggcgatg ccgacgccga gatattgttc gtcgccttct
     1201 gcaccgacgg tcgtgaacgg cagcacggcg agcgagcgcg tcttgcctgc gccgcctacg
     1261 tttgccgcgc ccgcgtcggt ggtggggaag gcgtcggcga gatagacctc aaaattaccg
     1321 tcgcgctgcg tctggaaagc gacgcggcgg ctatcgggcg accagccggg ttcgtcgtcg
     1381 aaggtgcgcg cgcccgtgat gaggcgttga ttgttgccgt ccgcgtccat gatatagacg
     1441 gcggggtagt cggtcttgtt cgggcggttg cccgcgaagg tgatgtgctt gccgtcgggc
     1501 gaccacgcgg gcttcgcatc gtcgccggga tggttcgtca ggttgcgcac gtcggagccg
     1561 tcggcgttca tcacgtaaat ctcataagac tttccatcgc gggcgctagt gaaggcgatc
     1621 ttctgtccgt cgggcgacca ggtggcgtcg ctgtcgaatc tggggtcgtc ggtcagcttg
     1681 atcttgtccg agccgtcggc gcgcatagtg aaaacgtcgt agttcgtggg cgcgtcgagc
     1741 gtgttgctgc cgtaggcgat gcgccggtcg tcgggcgacc atgatgggcg cgtgtcctcg
     1801 ccgggggcgt tcgtgaggtt gcgctggtta ctgccgtcgg cgtccatgac atagacttcc
     1861 atctgcccgt cgcgtcggct ctggaaagcg atcttcgtgc cgtcgtgcga ccacgcgggg
     1921 cagtcgtctt cggtggtgtt gtaagtcagg cggcgcacgt cggagccgtc ggcgcgcatg
     1981 gtatagattt cggtcgcgcc gtcgcggttg ctggcgaagg cgatgcgcgt cccgtcgggc
     2041 gagtagtcgg ggtgccagtc gtgtgcgatc tcggtcgtca gccgcagcgg gctggcgcct
     2101 gcgagcgcgg cgattgatct gttggaacgc ttcgcgtaga agaacgcccc cgtggtgacg
     2161 gcggcgagca cgacgagcgc caccgtcagt gcgctgccga ccgcgcgccg cgagagacga
     2221 cgctccggcg gcggcggcgc gggcttctct tgctccgcag gtttcttgtt gccgttcgtc
     2281 gtaaattcca gcgtgggcaa actggagagg tgcgggtcga attgcctcgc ttcacgtctg
     2341 gccgcctcgc ccgcgtagcg cgctgcgagc gggttgttcg tgcggcgcag gatttcggcg
     2401 aagcgtggat cggcgtgcag gttgtcgagg tgcggatcga cgcccgcgaa cgtgaagccg
     2461 acggcgtgtt cgttctcggc ctgcgcgagc cagtgaaacg cctcgtcgcg ttcgttgagc
     2521 agcgaataga tgacggcgat ctcgtaggag gtgacccatt gctcgtcgcg gcgcgcgagc
     2581 agttcgcgga gcagcgcgac ggcctcgtcg cggcgaccgc ccgccgccag tacgtgcgct
     2641 tctttcaggc gcgtcgtcgg cgtgtcgccc gcaaagacgc tctcctgctc gtaggtcgag
     2701 agagcctctt cgtgcatgcc tttctgctca taagcccagc gcaggatgac gtgtgcggcc
     2761 accgagccgg gatcgagttc gagcgcgtgt ttgcactgct cgatggcttc atcgaaacgg
     2821 cgcgcgagga agaggacgtt ggcgagcgca gtgttgttga cgggcgagtc cggcgcaatt
     2881 tgcagcgcgc gtttgatctc ggagatcgag tcgtcgtgcc gaccgatggc cgcgaggttg
     2941 aaggcgtacc agcgatgcgc gagcgcgttg ccg
//
LOCUS       DASWNS010000320         7189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_117347, whole genome shotgun sequence.
ACCESSION   DASWNS010000320 DASWNS010000000
VERSION     DASWNS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117347"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..568)
                     /locus_tag="VGB76_11135"
     CDS             complement(146..568)
                     /locus_tag="VGB76_11135"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY0099492.1"
                     /translation="MFCPVCESEYEKGIEHCPDHDVALVEQLSPETSRDRSEARFVPL
                     HNIGSPAEAEMVHDILTQNGVRSAVQSGSSDAFSPLLSTTAPGALVLVDERDLERAQE
                     LYNSFFGRDTTPLTGTTLEEDEDIASDDDTDDTDDTDG"
     gene            complement(709..1650)
                     /locus_tag="VGB76_11140"
     CDS             complement(709..1650)
                     /locus_tag="VGB76_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0099493.1"
                     /translation="MSLLVVGSVAFDAVETPFGKRERMLGGSATHFSLSASYFTDVRV
                     VGVVGADFGQAELDVFARHEIDTSDLERVAEGNTFFWRGRYDFDLNTAHTLDTQLNVF
                     ASFKPKLSEASRQSRFVFLGNIQPDLQTEVREGVSGAELVALDTMNYWINTVPDAVMA
                     AIRGVNVVIINDAEARQLANEPNLIRAARKILDAGPTALVIKRGEYGAALFTRDSYFA
                     IPAYPLESVFDSTGAGDTFAGGFMGYLASHETIDDATMRRAMIYGSVMASFNVEEFGT
                     ERVDRLTHEEINERFRTFKRMTHFEEIPFERAERAAK"
     gene            complement(1811..2284)
                     /gene="bamD"
                     /locus_tag="VGB76_11145"
     CDS             complement(1811..2284)
                     /gene="bamD"
                     /locus_tag="VGB76_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY0099494.1"
                     /translation="MKRVLLACCLLGVAFGVGANAAQAQEKPKSAEQELDANPLSDAL
                     MEVDAKKNLKAAQLYFTRKAYVGALQRCEETIAVHQRFKKFDEVLYIAGMSSLRLSRK
                     EGKQPPTLAPEKYLVLAREYLTQLVNEHPKSSFRKRAEEELQAIGQPAPQASEKQ"
     gene            complement(2370..3245)
                     /locus_tag="VGB76_11150"
     CDS             complement(2370..3245)
                     /locus_tag="VGB76_11150"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY0099495.1"
                     /translation="MTTAQIGIIGGSGLYQMPELKDVEEVNVETPFGNPSDAFIVGTL
                     EGVRVAFLPRHGRGHRYTPSELPFRANIYAMKLLGVESILSASAVGSLQEQYAPLDMV
                     IPAQFFDRTRARAHESTFFGSGIVAHVTFAHPVCDELGDVLEESCGAAEVKVHRGGTY
                     LCMEGPAFSTKAESNVYRSWGMDVIGMTNLQEAKLAREAEICYATLALVTDYDCWHED
                     HDAVSVETVVEYLNKNVRNAQVIMREAVKRLNERQRGCSCGSALKNAIFTAPDLWPEA
                     TTKKLEAIIKKYGGR"
     gene            complement(3281..3499)
                     /locus_tag="VGB76_11155"
     CDS             complement(3281..3499)
                     /locus_tag="VGB76_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099496.1"
                     /translation="MTQAKFIIEHPEQAKDDRQVYLAGVEGVLRAYTAVKGAKPKVKI
                     EPLEELLTKQPAGQLGEHVKSAMSKCKS"
     gene            complement(3552..4388)
                     /locus_tag="VGB76_11160"
     CDS             complement(3552..4388)
                     /locus_tag="VGB76_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0099497.1"
                     /translation="MQLGDYRVEIVPDAEFRLDGGAMFGVVPRALWSQSCPPDEHNRI
                     RLSTNCLFIEAGRERIIIETGIGDKWSAKHEAMYGITRRRPLKESVEAITGYSPAEIT
                     IVVNTHLHFDHAGGNTSRDAAGNLVATFPNARYFVSRGEYEHAESPHERDRASYLPEN
                     WRPLQASGQLELKEADYEVAAGLSMETVAGHSRTMQCVRLERGGQTLFSFADLVPMRA
                     HLPFAWIMGYDLYPVETLEAKRRLLPQAARENWLCLFYHDPDAPLCRIVEEDGKLRAA
                     GH"
     gene            complement(4484..5779)
                     /locus_tag="VGB76_11165"
     CDS             complement(4484..5779)
                     /locus_tag="VGB76_11165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0099498.1"
                     /translation="MAAIAVVAAGGLLYWQVKGRSDRGLSLSADDMSQIVKTMMPPQQ
                     LAALANNEEERKDIAKQFRQLLAIAADAKAAGLADSPEMKRQLELVRTFVIAQVYAKK
                     QQEAGATSQEQIVSKDEVANFLKEPGQDAKFEEFVKDVQTLGLLPAAQGLTDEQKERI
                     KNELWSPTQVLSRKAIAAGVDKERATELLIQFQQAQVLAQKHAPKIMERTKATDEEIN
                     AYIAKHPELDSSKTRAKAEDVLKRLRGGEDFAALAKEFSTDPSNKDKGGDLGWFKRGA
                     MVKPFEEAAFALQPGQISDIVETPFGFHIIQTQERRTAKGEDGQDTEEIHARHILIAA
                     DAPGGQANPFGPPQSPKEQARAAVEKEKREKLLDEIAERNGVTVAENFKVEAPPAPPR
                     GMQPPMGDPHGGGGEGLPPEATQPAAPEGSQQAAPRPRK"
     gene            5769..6065
                     /locus_tag="VGB76_11170"
     CDS             5769..6065
                     /locus_tag="VGB76_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099499.1"
                     /translation="MAATIKAFVVALKPRDVAAAARGDEDVRMLVEADEAAPVVSAVT
                     PVYDVSAFDSTVAVVVALRSSFEAVSPGHCCPAAFEVSFAVSVVSSAPQPGQVC"
     gene            complement(6193..>7189)
                     /locus_tag="VGB76_11175"
     CDS             complement(6193..>7189)
                     /locus_tag="VGB76_11175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0099500.1"
                     /translation="GAASPEQVVSKEEIAAFLKEAGQEQKFQEFYQDYLKSRQGAEGA
                     NAITDEQRENLRQQWAGIILSARKGVAAGVDKERATEVMLMYQHARLLAGEYFKQTLD
                     ARTKATDAEIEAYYAAHPELDPKQTKAKAEELLARLRAGGDFNALAKEFSVDTSNKDQ
                     GGDLGWFGRGMMVKPFEDAAFALKPGELSGIVETQFGYHIIKLDERRMQDSPNGQPVE
                     QIHARHILIAPGGAGRGANGRPQSPREQARGAVEGEKRNKLLGEIVSRTRIRIADDFD
                     PNGALVTTTAPGGAARTGDKPAAATTNASPTPSASTQTKGTSTRTRAGSSRRRRP"
BASE COUNT         1250 a         2300 c         2055 g         1584 t
ORIGIN      
        1 ccgtgatcgc tgcatcactc gcctcttgca tgctcgcacc tgacttctcg cacctgactt
       61 cccggtcgtc cttcaacatc caaacatccc gacgttatcg gactcttcaa ctgcgtgcgc
      121 taccgcgacc cgaagggcgt ggcgctcaac cgtccgtgtc atccgtgtcg tcggtgtcgt
      181 catccgaggc gatgtcttcg tcttcttcga gcgtcgtgcc cgtcaggggc gtcgtgtccc
      241 tgccgaagaa ggagttgtaa agttcctgcg cgcgctccag atcacgttcg tcaacgagca
      301 cgagtgcgcc cggcgcggtc gtggacagga gcggcgagaa ggcatcggaa ctgcccgatt
      361 gaactgccga ccgcacgccg ttctgcgtga gaatgtcgtg caccatctcg gcctctgcgg
      421 gcgacccgat gttgtgcagc ggcacgaaac gcgcttcgct ccggtcgcgc gaagtctccg
      481 gcgacaactg ctccactaag gccacatcat gatcaggaca atgctcgatt cccttttcat
      541 actccgactc gcacacggga caaaacataa attttcctca actaaatttt cgtggtggtt
      601 attaacccgc acagtttaag gggtgaaagg gtaaggggta aagggaaaag gtaaagagac
      661 acattctctt ccctctccct ttacccctaa acacttctcg ccctgtctct atttcgccgc
      721 gcgctccgcg cgttcgaaag gtatctcttc aaagtgggtc atgcgcttga aggtgcggaa
      781 acgctcgttg atctcttcgt gcgtcagacg atccacgcgc tccgtcccaa actcttcgac
      841 gttgaaagag gccatcaccg aaccgtagat catcgcacgc cgcatggtcg cgtcgtcaat
      901 cgtttcgtgg ctggcgagat agcccatgaa gcctccggcg aaggtgtcgc ccgcgcccgt
      961 cgagtcgaag accgattcga gcgggtaggc gggaatggca aagtaggaat cgcgcgtaaa
     1021 cagagccgcg ccgtattcgc cgcgcttgat gacgagagcc gtcggtcctg cgtcgagaat
     1081 cttgcgcgcc gcgcgaatga ggttcggctc gtttgccaac tggcgcgctt ccgcgtcgtt
     1141 gatgatcacg acgttgacgc cgcgaatcgc cgccatcacg gcatcgggca cggtgttgat
     1201 ccagtagttc atcgtgtcga gcgcgacgag ttccgcgccg ctcacgcctt cgcgcacttc
     1261 cgtttgcagg tcgggctgga tgttgccgag aaagacgaag cgcgactgcc tggaagcctc
     1321 ggaaagtttc ggtttgaaac tggcgaagac gttgagttgc gtgtcgagcg tatgcgccgt
     1381 gttcagatcg aaatcgtaac gcccgcgcca gaagaatgtg tttccttcgg ccacgcgttc
     1441 gaggtcgctc gtgtcgatct cgtggcgcgc gaacacgtcc agttccgcct gaccgaaatc
     1501 cgcgcccacc actccgacca cacgcacgtc tgtaaagtaa ctggcggaga gcgaaaaatg
     1561 tgtggccgac ccgccgagca tccgctcgcg ctttccgaac ggcgtctcca ccgcatcgaa
     1621 cgccaccgaa ccaacaacca gcaatgacac cttgtgtttt ctcctgtgtg tgaaatgaaa
     1681 agaccgtcag ttaccagtaa gcccgtagcc cgtcggatgt gcttctcaca cgagtcgcta
     1741 aaaaccagca tggcacgacg ggcgacgaac ggagagcaac tgacctaagg acacatgccg
     1801 acccacattt ttactgcttc tcgctcgcct gcggcgcggg ttggccgatg gcttgaagtt
     1861 cttcttcggc gcgcttgcgg aaagaactct tcggatgctc gttaacgagt tgggtcagat
     1921 actcgcgcgc cagaacgaga tatttttcgg gggcgagcgt gggcggctgt ttgccctctt
     1981 tgcgcgagag gcgcaggctg ctcatccccg cgatatacaa gacctcatca aacttcttga
     2041 agcgttgatg gacggctatc gtctcctcgc agcgctggag cgcgccgacg taggctttgc
     2101 gtgtgaagta caactgcgcc gccttgagat tcttcttcgc atcaacttcc atcagcgcgt
     2161 cgctcagagg gttcgcgtcc aactcctgct ccgcagactt cggcttctcc tgcgcctgcg
     2221 ccgcgtttgc ccccaccccg aaagcgaccc ccagcaaaca acacgccagc aaaactctct
     2281 tcataaaaac acctctctca aatagacgta agtgacgcac ggcaagcgtt gaaccgtatc
     2341 gagtcttaaa tctctctaca cgtcacacgt tacctgccac cgtacttttt gatgatggct
     2401 tcgagcttct tcgttgtcgc ctccggccac aaatccggcg cggtgaagat ggcatttttg
     2461 agcgcggagc cgcacgagca gccgcgttgt cgctcattca gacgcttcac ggcctcgcgc
     2521 atgatgacct gcgcgttgcg cacgttcttg ttcaaatact cgaccaccgt ttcaaccgac
     2581 acggcatcat gatcttcatg ccagcagtcg taatcggtga cgagcgcgag cgtggcgtag
     2641 cagatttcgg cttcgcgcgc gagtttcgct tcctgcaaat tcgtcatgcc gatcacgtcc
     2701 atgccccacg agcgatagac gtttgattcg gctttcgtcg aaaacgcggg tccttccatg
     2761 cacaggtaag ttccgccgcg atgcactttc acctccgccg cgccgcagga ctcttccagc
     2821 acgtcgccga gttcgtcgca gacgggatgc gcgaacgtga cgtgcgcgac gatcccgctg
     2881 ccgaagaagg ttgactcgtg tgcgcgtgcg cgcgtgcggt cgaagaactg cgcggggatg
     2941 accatgtcga gcggcgcata ctgctcctgc aaagagccga ccgccgaagc cgacaggatg
     3001 ctctccacgc cgagcaattt catcgcatag atgttggcgc ggaagggtaa ctccgaaggc
     3061 gtgtagcgat ggccgcgccc gtgccggggc aggaaggcga cgcgcacgcc ttccaacgtg
     3121 ccgacgataa aggcgtccga cggattgccg aacggcgtct caacattcac ttcctcaacg
     3181 tctttcaatt ccggcatctg gtagaggccg ctgccgccga tgatgccgat ctgtgctgtc
     3241 gtcatagtct gtccgtgatc ctctttaggt atgcctgctg ctaagactta cacttgctca
     3301 tcgcgctctt gacgtgctcg ccaagctgcc ccgccggttg cttcgtcaac aactcttcaa
     3361 gcggctcgat cttcactttg ggcttcgcgc ctttgaccgc cgtgtaagca cggagcacac
     3421 cttcaacgcc cgcgagatat acctgccggt cgtccttcgc ctgttcggga tgctcaatga
     3481 tgaacttcgc ctgcgtcatc tctgaccgct gccaatcggg gcgacagcgc gcgaaactgt
     3541 ctcctgttct cttaatgacc ggcggcgcgg agctttccgt cttcttcgac gatccggcag
     3601 aggggcgcgt ccgggtcgtg ataaaaaagg cagagccagt tttcgcgtgc ggcttgcggc
     3661 agcagacgcc gcttggcttc gagcgtctcg accggataga gatcgtatcc catgatccag
     3721 gcgaaaggga gatgcgcgcg catcgggacg agatcggcga agctaaagag cgtctgcccg
     3781 ccgcgctcca gacgcacgca ctgcatcgtg cgcgaatgtc cggcgacagt ctccatgctc
     3841 aagcccgccg ccacctcgta atccgcctct ttcaactcca actgcccgct cgcctgcaaa
     3901 ggccgccagt tctccggcag ataactggcg cggtcgcgct cgtgcggcga ctccgcgtgt
     3961 tcatactcgc cgcgagagac gaagtaacgt gcgttcggaa aagtcgcaac gaggttgccc
     4021 gccgcgtctc tgctcgtgtt gccgcccgcg tgatcgaagt gcaaatgagt gttgacgacg
     4081 atagtgatct ctgcgggact gtagcccgtg atggcctcga ccgactcttt gagcggacgc
     4141 ctgcgcgtga tgccatacat cgcttcgtgc ttcgcagacc acttatcgcc gatgcccgtc
     4201 tcgatgatga ttcgttcgcg ccccgcttcg atgaacaggc agttcgtgct caggcgaatg
     4261 cggttgtgct cgtcgggcgg gcagctttgc gaccacaggg cgcgcggcac aactccgaac
     4321 atcgcccctc cgtccagacg aaactccgca tccggcacga tctccacgcg atagtcgccg
     4381 agttgcataa ccaagctgtt aacgattagc tgccggttga gagcggaacg ggcgaaaaga
     4441 gactgcgccg cgtttcgcta acaattgaca gctaatcgct ccttcatttc ctcggcctcg
     4501 gagcggcctg ctgcgagcct tctggtgcgg caggctgagt cgcttcgggg ggcaagcctt
     4561 ctccgccgcc tccgtgcggg tcgcccatcg gcggctgcat tccacgcggc ggcgcgggcg
     4621 gcgcttccac tttgaagttc tccgcgacgg tgacgccgtt gcgctccgcg atctcgtcca
     4681 gaagtttctc gcgcttctct ttttccacgg cggcgcgcgc ctgctctttg ggcgactgcg
     4741 gcggcccgaa cggattggcc tgcccgcccg gcgcgtctgc ggcgatcagg atgtggcggg
     4801 cgtgtatctc ctctgtgtcc tgcccgtcct cgcccttggc cgtgcggcgt tcctgcgtct
     4861 ggatgatgtg gaagccgaaa ggcgtctcga cgatatcgct gatctgtccg ggctgcaacg
     4921 cgaaggccgc ttcctcgaaa ggcttcacca tcgcgccgcg tttgaaccag ccgaggtcgc
     4981 cgcctttatc tttgttgctc gggtcggtgg agaactcttt ggcgagcgcg gcaaaatctt
     5041 cgccgccgcg caggcgtttg agcacgtctt cggctttggc gcgcgtcttc gaagaatcca
     5101 actcaggatg ttttgcgatg taagcattga tctcttcgtc ggtggccttc gtgcgttcca
     5161 tgatcttcgg cgcgtgtttc tgcgcgagca cttgtgcctg ctggaattga atcaagagtt
     5221 cggtggcgcg ctctttgtcc accccggcgg caatcgcctt gcgcgagagt acctgcgtgg
     5281 gcgaccagag ttcatttttg atgcgctcct tttgctcgtc ggtcaacccc tgcgcggcag
     5341 gcagcagccc cagcgtctgc acgtctttga cgaactcttc gaacttcgcg tcctgtcccg
     5401 gctctttcag gaaattggcg acctcgtcct tcgagacgat ctgctcttgc gaggtggcgc
     5461 ccgcctcctg ctgtttcttc gcataaacct gcgcgatgac gaacgtgcgc accaactcaa
     5521 gctgtcgctt catctccggc gaatcggcca gccccgcggc cttcgcgtcc gccgcgatgg
     5581 cgagcaactg gcggaactgc ttggcgatgt ccttgcgctc ctcctcgttg ttggcgagcg
     5641 cggcaagttg ctgcggcggc atcatcgtct tgacgatctg cgacatatca tccgcagaga
     5701 gactaagccc cctatcgctg cgccccttca cctgccagta gagcaaccct cccgccgcca
     5761 cgacggcgat ggcagccacg atcaaagcct tcgtcgtcgc actcaaaccg cgtgacgtgg
     5821 cggctgcggc gcgcggcgac gaagacgtac ggatgcttgt cgaggcggac gaagcagccc
     5881 ccgttgtctc ggctgtcacg ccggtatacg acgtttcggc gttcgattcg acagtcgccg
     5941 ttgtcgtcgc gctcaggtct tcatttgaag cagtctcgcc cggccattgc tgcccggcgg
     6001 cgtttgaagt ctcattcgca gtatcagtag ttagttccgc accgcaaccg gggcaggtgt
     6061 gctgatcttc cgacgtttgc gttccgcatt tctcacaagt gatcataaac tcatcctctt
     6121 aaataacagg ttcaacaacc tttcaatttt tgcgtctctc gaaacgtagc gcgcggcgca
     6181 agagcttgct tgctacggtc gcctgcgtct cgaacttcct gcccgtgtgc gtgtgctcgt
     6241 gccttttgtt tgcgtcgagg ccgacggcgt cggtgaagcg ttcgtcgtcg ccgccgcagg
     6301 tttatcgcct gttctggccg cgccgcccgg cgcagtcgtc gtcacaagcg caccattggg
     6361 gtcgaaatcg tctgcaatcc tgatgcgggt acgactcacg atctcgccga gcagtttgtt
     6421 gcgcttctcg ccttcgaccg cgcctcgcgc ctgttcgcgc ggagactgtg gccgcccgtt
     6481 tgcgcccctg cctgcgccgc ccggcgcgat caaaatatgg cgcgcgtgaa tctgctcgac
     6541 gggctgcccg ttcgggctgt cctgcatgcg ccgttcgtcg agtttgatga tgtgatagcc
     6601 gaactgcgtc tcgacgatgc cgctcaactc gccgggcttg agcgcgaagg ccgcgtcttc
     6661 aaacggcttg accatcatcc cgcgcccgaa ccagccgagg tcgccgccct gatccttgtt
     6721 gctcgtatcg acggagaact ctttggcgag cgcgttgaaa tcgccgcccg cgcgcaggcg
     6781 cgccagcaac tcttcggcct tggctttcgt ctgtttcgga tcgagttcgg gatgcgccgc
     6841 gtagtaggct tcgatctccg catcggtcgc tttggtgcgc gcgtccagag tctgtttgaa
     6901 atactctccg gccagcaggc gcgcgtgctg gtacatcaac atcacttcgg tggcgcgttc
     6961 tttatccacc ccggcggcga cgcctttgcg cgcgctcaag atgatgccag cccattgctg
     7021 ccgcaagttt tcgcgctgct cgtccgtgat cgcgttcgcc ccctccgccc cctgacggct
     7081 cttcaaatag tcctgataga actcttgaaa cttctgttcc tgccccgcct ctttgaggaa
     7141 agccgcgatc tcctctttgg agacaacctg ctccggcgac gccgcgccc
//
LOCUS       DASWNS010000321        19800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_221763, whole genome shotgun sequence.
ACCESSION   DASWNS010000321 DASWNS010000000
VERSION     DASWNS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19800
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221763"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2447
                     /locus_tag="VGB76_11180"
     CDS             <1..2447
                     /locus_tag="VGB76_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099501.1"
                     /translation="PREAFAHFPMPRATDLFGPTFRGEGTIRIDDVKLDPRYGKNSPY
                     YGMPEGHLPVTSYLAVPVVSRSGLVIGGLFFGHEKAGVFNERSARIVEGLAAQAAVAM
                     DNARLFDTAKTERAKAEASERYYRFLAETIPQIVWTAQPDGSVDYYNERWYEYTGLTL
                     DQTQGWGWDAVLHPNDVQPCLDRWKQSVETGEIYEIEYRFRRASDGQYRWHLGRALPL
                     RDGDGKIVKWFGTGTDIDDHKRSEEAQRFLAEASAVLVASLDYEATLAALARLIVPTL
                     ADWCTIDMITDERTIRRLAVAHQDPAKVELAWELERLYPQQMGDTEGVAKVIRTMRPE
                     LYPNVPEELLALIARDAGHLKILRELGLRSALVVPLIAHNRVLGAISLIGAESGRRFT
                     SADLDFAEDLAHRAALAVDNARLYRDAQEVNRLKDEFLATLSHELRTPLTAVLGWTRL
                     LGTNQLDEATSRRALETIERNAHAQVQLIDDILDVSRIIRGKLRLNVRPCELTPVIEA
                     AVDSVRPAAEAKGIRLHVVLDPRAGPVSGDPDRLQQVVWNLLTNAVKFTPKDGRVQVL
                     LRRVNSHLELTVSDTGQGIEEKFLPFVFDRFRQADPSTTRVHGGLGLGLAIVRHLVEL
                     HGGTVTVESRGKGEGTTFKVALPLMIMYQPESAPASADDADSTHPTAGGHLTLACPPE
                     LEGLRVLLVEDEPDARELLVAVLTQCRADVRAVSNVADALSELEAWQPEVLISDIEMP
                     GEDGYSLIRKIRNLPPARGGKIPAAALTAYARAEDRMRALLSGFQLHVPKPVEPAELV
                     AVVASLAGRATEKERG"
     gene            complement(2551..3105)
                     /locus_tag="VGB76_11185"
     CDS             complement(2551..3105)
                     /locus_tag="VGB76_11185"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY0099502.1"
                     /translation="MGFKIGQKVVYPNHGIGTIEQVEQKQIGAIAIPFYTLRLAATNS
                     VVLVPVANASEVGLRSPISSAECEMLLKNLGDDFAAAPQDWKDRFKEFSEKMRTGDIF
                     EVADVLKHLTYLSHSKPLSFREQRMLERARYLVVSELAAVCRQPECNVEPRVEQALTR
                     ACVKHERRPAAAGRTRAAGAVAGH"
     gene            complement(3486..4526)
                     /locus_tag="VGB76_11190"
     CDS             complement(3486..4526)
                     /locus_tag="VGB76_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0099503.1"
                     /translation="MSVTLAFVIFIIFIALVFDYINGFHDAANSIATVVSTRVLSPGM
                     AVVWAAFFNFIAFAVLGTRVAKTIGGDLVRIELIQSDMRLYVLLAALLGAVIWNLITW
                     YLALPTSSSHALVGGYAGAAIAAYGGFEGLLKPEGWIKTLVFIIASPLIGMSLGFFMM
                     VAVFWMFRRSTPTRVDKLFRRGQLLSAAAYSLGHGGNDAQKTMGIITIVLVTSNLLQA
                     GEGGKLPEIPLWVVLTAHAAIAAGTLSGGWRIVHTMGSKITKLKPVGGFCAETAGALT
                     LFGATLAGIPVSTTHTITGAIVGVGATRRLSAVKWGVAGRIVWAWVLTIPIAALIAAL
                     SFLFIRFLDRLT"
     gene            complement(4634..5263)
                     /locus_tag="VGB76_11195"
     CDS             complement(4634..5263)
                     /locus_tag="VGB76_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY0099504.1"
                     /translation="MARFSFLPREDQYFSFFSQMTSYIFDASKILVEMMSDSSSSRDY
                     NAYAQRIKTIEHACDELTHTISTSLNKSFITPFDREDIYMLSSALDDVVDLIDDAARA
                     MVMFNIQESTSHARAFADVIQRMAVQLHEIVSTLARPTGINQRLVEIHRLENVADDTY
                     HAAIGELFRRETDPLTVLKFKEIYEKLEAAVDRCEQVANIIESVIIKNA"
     gene            complement(5621..7438)
                     /locus_tag="VGB76_11200"
     CDS             complement(5621..7438)
                     /locus_tag="VGB76_11200"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEY0099505.1"
                     /translation="MIHPVRCALLASALFVALAPSAFAQTQAAAHAAASDPNAQKTRA
                     DAPQQTTPGDLSLDDVRRQLQQQRTEIEQLRSTLAEQTRLLNELLARIARTEPDAASN
                     VNGANVREAVYSSNAAAATETLRADPPAAQEAGTKNPQTEGIDTRVRAIEAQAQKTTE
                     TLARQLGSITFSGDLRLRYEGTFGQLNALANADNPAISGNELSTRHRARLRARLALRG
                     QLGKEFDWGLRFSTGTLADVSSSNQTLTDFFNRKQFALDQAYLTYRPARIPGLRLQGG
                     KFETPWLFTEMTFDADIQPEGINESYSRDFKGDVLKNLTLVAWQLPMLERQSTFVLGA
                     DGRASLDASRRGGRDLALYGAQARTRLALGKQAALTLSAADLYFSGTQFITPAQFFGG
                     QLQVPVTVALPATGETPAQTVTGFAAIPRELLVAGSSLGVSLANTNAVNRDGRLSSGF
                     NLVDLIARLELTHSRRFPVTLLFNFVTNTQTHDVVVAGAGGANRLVPNDESNGYWAEI
                     QVGKARERGDLLFNYTLMRIEKDAVLTPFNFSDILQPSDVRAHRMIFNYTVDPRVTLS
                     LTGLVSQRPHGLAGVFGTTPAGSLNRPTSRLQLDTIFRF"
     gene            8078..9172
                     /locus_tag="VGB76_11205"
     CDS             8078..9172
                     /locus_tag="VGB76_11205"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY0099506.1"
                     /translation="MQKTRALMGFDALRNLVSDSMAIDLGSASTIISVRGRGIVVDEP
                     SVVAVSKTTGEIVAIGREAHTMQGREARDVTLVMPMVDGVVADFERSQRMLDHFVREA
                     RSGISHFSRRAVMSVLSGVTHVEQRALMAAAEAAHIGRVYMVEEGLAAAIGAGVSVEE
                     QKASAVVDIGGATTNVAVVALGMIVYAQAERVGSSDIDEAIMDRLRRHHGLIIGAPTA
                     ERLKIALGSAVEPKDPLRTLTVKGRDVQTGTPRAIEVTSDEVCSAAQNVVQRIVRVVQ
                     RGVAELSPEVAADIYDRGLMLTGGGALLDGMAAFLQSETRLVTRMAEDPRHAIVHGLS
                     QLFDDPLLLRRVARNEPSLLLDADAGAFET"
     gene            9197..9637
                     /locus_tag="VGB76_11210"
     CDS             9197..9637
                     /locus_tag="VGB76_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099507.1"
                     /translation="MRSQIEIAIAAALSVDLPLYWMFTRRDSLGRPNPLTSGEAAVLL
                     LVLACYLSVPYMVGSFRRRRVLRAVPLWFWVSVLGAALILTADYSIGIRYGLSTWEGV
                     GKDWFALTVFTLPVTALVYYSGAIVGLIKEWHFDRRDNLRIVRR"
     gene            9712..10836
                     /locus_tag="VGB76_11215"
     CDS             9712..10836
                     /locus_tag="VGB76_11215"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0099508.1"
                     /translation="MSLTHILHRQLRVARVYGIPVRIDYRWFMVFALSTWLIASNFEQ
                     GTPLVRYVNVTPSTAWVVGLVTTLGLFLSIFGHELSHALVGRIEGIETEEIVLHPFGG
                     MARLSREPDNARAEFRIAVAGPASSFLFSVAAFGAMMLAAAFEQRLAGAVFFIIGFWN
                     LMLAVFNLLPGYPLDGGRVLRAFLWHRNGKLEEATRIASLGGQLIAWTLFVFALLLYF
                     KTGDLFMALWSVVVALFLHSAARSVGTGRKYLETVGEAMSAPIAVEPDVLVSHFLDLF
                     LPQHPQEAFPVARGRRLHGILTLADVRNLPEASLHRTRVADVMRPVAPELFVDPLTPL
                     SRADALMKQNGAGALAVLDGAGELIGFLHRGRLKRRKETE"
     gene            11171..11317
                     /locus_tag="VGB76_11220"
     CDS             11171..11317
                     /locus_tag="VGB76_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099509.1"
                     /translation="MINAHANFRNELATDDIEQAEPKGDMIFLETARERTAQQSTPLP
                     QQIG"
     gene            11463..12323
                     /gene="cdaA"
                     /locus_tag="VGB76_11225"
     CDS             11463..12323
                     /gene="cdaA"
                     /locus_tag="VGB76_11225"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEY0099510.1"
                     /translation="MFFAPTYIEYLDRLREPRNLVDIALVFLIVYTVLKLLRGTRAVP
                     MAVGIAFFALLYWIAYNRDLTTLEFVLRPAPLYLGIAIIVLFQTEIRQALISLGNQIR
                     LPGLARHRGQFGEGVYDEIVLAATTLASTKTGALIVIERGVGLKNVTDGGVKMDAELS
                     YDLLVTIFNPATPLHDGAVVIRRHRVAAAACFLPLTLNPRLSKDLGTRHRAAIGITED
                     TDAVAVVVSEETGLISFVIGGQIRRGLDATKLRAAIFDAIKTPARTANEEPATLSADA
                     ESERNEVVST"
     gene            12414..13352
                     /locus_tag="VGB76_11230"
     CDS             12414..13352
                     /locus_tag="VGB76_11230"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEY0099511.1"
                     /translation="MTLREVERDAAQMARRWLNDLLFADWGLKLLALAITLGLWFAVT
                     GQRVPTTARLRKVPLFFVLPNEMEISNEPRDEVDLTLRGNKRVLDAIKASDLSLSYDA
                     SSYRPGERVVQLSPNNITLQLPVGTSAEDVTIERIEPGSVPLRLERRIERELEVEPRL
                     EGKLPEGFELTGTQVLPARVRVRGPESHVNALERASTETILLEGRRETFTAPQTAIDI
                     QDRKLVTLDAVVDVIVKISETQATKRLAGVAARTIDGSDTRLTIEVRGARSVVEPLRA
                     SDLSVVFDTAADGTQKPRLVLPSHLEGRVELISTNQ"
     gene            13432..14868
                     /locus_tag="VGB76_11235"
     CDS             13432..14868
                     /locus_tag="VGB76_11235"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY0099512.1"
                     /translation="MHKLFGTDGMRGEAGRFPLDAPTIETVGRSLARQLAECAGGEGR
                     APRIIVGRDTRESGEWIERAFLYGARAAGASCESAGIITTPGVAFLTRTLPAEAGVVI
                     SASHNPYHDNGIKIFAPTGRKLDDATERRIEADIRQAHGEGRDPRVEARAKRDEADAN
                     GQRTGASTPRSVGGAGDAAQRAAALQARYLDYLSEEVAPDLRLDNLRLVLDCANGAAS
                     QFAPALFERLGAEVFATGQTPDGRNINHECGSLYVEHLREQVLTNHAHLGVAFDGDAD
                     RALFLDARGQVVDGDATLWVMAKSLQARGELAGEQIVATVMSNIGLELALGSREIKML
                     RADVGDKYVLEELLRTGASLGGEQSGHIIFPRVSLAGDGMITTLFLLRAMQESGESLE
                     ALTAGFTRYPQVLVNVRVAEKRPFAEVAEIAVEARRTEAALDGQGRLLLRYSGTEPLA
                     RVMIEGRHQEDIERLAHALAAVIERALG"
     gene            15319..16872
                     /locus_tag="VGB76_11240"
     CDS             15319..16872
                     /locus_tag="VGB76_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099513.1"
                     /translation="MKTGDYRRDYAAYAAALERARYDYHTGVTPELRLTPIRDRYADL
                     WTRDAVNDLTRAREEISRQFETERVACAALLRAAQLGYVEAQATDVTRELARCRASAV
                     VEWNGASLAPEDAPDALALEPDAARRRELSARWFEALRACNDLRAARFDTLREAARTL
                     NFNSYFEFLSGIHEESSLERLSAAADNFLARTTSVYNSTLFEWAARHLPPEFSRAPAY
                     ADSLFLRRLSHLDPFFPASELRATYEATMQDLGVRAGQQHNLSIEATGHKIGSMLAAC
                     YALRPPEEVRLVHRSEGGASLYQMFFAAAGGAQAFAWVSRDLAARYPELVYAPDETTR
                     AGFQLLFADLLADAAWLTAHRNVRPSSAGEIARSFAFVELQLTRRAAVRLQQQRILDQ
                     ARDPLDAQLAEGYAVALEEAINFRPDPSLYLRDLISDDPEETRASARLQPAVYLRARL
                     FATALGEYFRTRYGHRWWAARRAADELIDLWNTGSRYTVEELASLAGLGDLNFDLLAD
                     TTEAALRGE"
     gene            16893..18056
                     /gene="aroB"
                     /locus_tag="VGB76_11245"
     CDS             16893..18056
                     /gene="aroB"
                     /locus_tag="VGB76_11245"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY0099514.1"
                     /translation="MRLPARENYEIEIGANALRRLGDIVRRALPSEARRIALVSNRRV
                     FELYGAGVLRSLRAGGFQVSHWLMGDGEQHKNLRNAERALAFFSESRLERSDAVVALG
                     GGVVGDLAGFAAAVYLRGIAFLQVPTTLLAQIDASVGGKTAVNTREGKNLIGAFHQPR
                     AVVISTETLQTLPRREMTAGWCEAIKQGAVGDRRLYEQTRRFLVAENENEKARGSKSE
                     RGAKQRGGRADELAALIAAQCAFKAKIVAGDEREAVGRTDARSRRILNFGHTSAHALE
                     AVTAYRRFRHGEAVGYGMIVAAEISHRLGMLDGSELESLRACVGLAGRLPRADDLEER
                     ALLRVIASDKKSVGGQVRWVLLEGIGRARIVGGQEIPPRIVRAALHAALVRQP"
     gene            18079..18486
                     /locus_tag="VGB76_11250"
     CDS             18079..18486
                     /locus_tag="VGB76_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099515.1"
                     /translation="MSQVRDENFRARWTTERGSSRFNFLITVVVIALVGYAAYQYVPV
                     AYQASQLKVSMQDTVDKAVVTSKNAAWAEEQLRRSLPIYGAPPNTLITVSPREARLEA
                     HVQYSVPIPLLVTTYEYKFDHTTRSAYLMSGGG"
     gene            complement(18597..>19800)
                     /locus_tag="VGB76_11255"
     CDS             complement(18597..>19800)
                     /locus_tag="VGB76_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0099516.1"
                     /translation="ENPKEGSLKRAMRRGDTEIAESLRAREVFAPSTVVWAPEAEMGE
                     RFLIEISRGCSQGCRFCWAGFNYWPPRVVPARDVLAKAAEWRGKTDKIGLVSTAVCDH
                     PEISEILQGLREMNFRISVSSLRLDQIEDELLDALVESRDQQIAVAPETGSDRLRKVI
                     NKNLTNDEIVEICGAVFDRGMLTIKLYLMVGLPTETDEDLAEMVRLVERIKDRMLEAG
                     RRFGRVGKIIPALNGFVPKPNTPFQWEPICEERELKRRLKWVSKQLARIPNVEVRAMS
                     ARIAHEQALFSSGDRSVSRVIEAMAQNGGDLGAALRETNFDPAFHTSRTRTYEEFLPW
                     EIVDAGLGRAFMQREHERAQIAKSTAPCPSVPTCERCGVCPTTWLASAPASLVQLQSS
                     LGAKVANA"
BASE COUNT         3765 a         6019 c         6284 g         3732 t
ORIGIN      
        1 tgccgcgcga agccttcgcg cacttcccca tgccgcgcgc cacagacctc ttcggtccta
       61 cctttcgcgg cgaaggcacg atccgcatcg acgacgtaaa actcgatccg cgctacggca
      121 aaaactcgcc ctattacggc atgccggaag gccacctgcc cgtcacgagt tatctggccg
      181 tccccgtcgt ctcgcgttcg gggttggtga tcggcgggtt gttcttcggt cacgaaaagg
      241 ctggcgtctt caacgaacgc tccgcgcgca tcgtcgaagg actcgccgcg caggccgccg
      301 tcgcgatgga taacgcgcgc ctgttcgaca cggcgaagac cgaacgcgcc aaagccgagg
      361 ccagcgagcg ttactaccgt tttctcgccg agacgatccc gcagatcgtc tggacggcgc
      421 aacccgacgg ttccgtggat tactacaacg aacgctggta cgagtacacc ggcctgacgc
      481 tcgaccagac gcagggctgg ggctgggatg ccgtcctgca cccgaacgac gtgcagcctt
      541 gtctcgaccg ttggaagcag tccgtggaga caggcgagat ttacgagatc gaatatcgtt
      601 tccggcgtgc gagcgacgga cagtatcgct ggcatctggg tcgcgccctg ccgctgcgcg
      661 acggggacgg aaagatcgtc aaatggttcg gcacgggcac ggacatcgac gaccacaagc
      721 gcagcgaaga agcgcaacgc tttctcgcgg aggcgagcgc ggtgctcgtc gcctcgctcg
      781 attacgaagc gacgctcgcc gcgctcgcgc gtctcatcgt gccgacgctc gcagactggt
      841 gcacgattga catgatcacg gatgagcgca cgatccggcg tctcgccgtc gcgcatcaag
      901 accctgcgaa agtagaactg gcgtgggaac tcgaacgcct ctacccgcag cagatgggtg
      961 acacggaagg cgtcgccaaa gtgatccgca cgatgaggcc ggaactctac ccgaacgtcc
     1021 ccgaagaact cctcgcgctc atcgcgcgcg atgccgggca tctgaaaatt ctgcgcgaac
     1081 tcggactcag atcggctctc gtcgtgccgc tcatcgcgca caatcgagtg ctcggcgcga
     1141 tctcgctcat cggcgcggag tcgggcaggc gtttcacctc cgccgatctc gatttcgccg
     1201 aagacctcgc acaccgcgcc gcgctcgcag tggacaacgc gcggctctac cgcgacgcgc
     1261 aggaggtcaa ccgtctcaag gacgaattct tagcgacgct ctcgcacgaa ctgcgcacgc
     1321 ccttgacagc cgtgctcggc tggacccgcc tcctgggcac gaatcaactc gacgaagcga
     1381 cctcgcgccg cgcgctcgaa accatcgaac gcaacgcgca cgcgcaggtg caactcattg
     1441 acgacatcct cgatgtctcg cgcatcattc gcggcaaact gcgcttaaac gtgcgcccct
     1501 gcgaactcac gcccgtcatc gaagcggcgg ttgattccgt gcgcccggcg gcggaagcca
     1561 aaggcatccg cctgcacgtc gtactcgacc cgcgcgcggg tcccgtctcc ggcgatcctg
     1621 atcgcttgca gcaggtcgtc tggaatctac tgacgaacgc cgtcaagttc acgccgaaag
     1681 acgggcgcgt gcaggtcttg ctcaggcgcg tcaactcgca tctcgaactc accgtgagcg
     1741 acacggggca gggcatcgaa gagaagtttt tgcctttcgt cttcgaccgt ttccggcagg
     1801 ccgatccttc gaccacgcgc gtgcacggcg gcctcggcct cggcctcgcc atcgtccgcc
     1861 acctcgtcga actgcacggc gggacggtca cggtggagag tcgcggcaag ggagagggca
     1921 cgactttcaa ggtggctctg ccattgatga tcatgtacca gccggagtcg gctcccgcca
     1981 gtgcggacga cgccgactca acgcacccga ccgccggagg ccacctgacg ctcgcctgcc
     2041 cgcccgaact cgaaggtctg cgcgtgctcc tcgtcgaaga cgaaccggat gcgcgcgaac
     2101 tgctcgtcgc cgtgctcacg caatgtcgcg ccgacgtgcg ggcggtgtcg aacgtcgccg
     2161 acgccctctc ggaactcgaa gcgtggcagc ccgaagtttt gatcagcgac atcgagatgc
     2221 cgggcgaaga cggctacagc ctgatacgca aaattcgcaa cctcccgccc gcgcgcggcg
     2281 gcaagattcc cgccgccgcc ctgacggcct acgcacgcgc cgaagaccgc atgcgcgcgc
     2341 tgctctccgg cttccaactc cacgtcccga agccggtcga accggcggaa ctcgtcgccg
     2401 tcgtcgccag cctcgccgga cgcgccacgg aaaaagagag aggttaaagc taaaagggga
     2461 agagggaaaa ggggaagagg taagggaaag acacacggtt gagtgcctgc tcccttaccc
     2521 cttccccttt aatctttgcg ctaaagtttt ttagtgtccg gcgactgctc cggcagcgcg
     2581 cgtgcgtccc gctgcggcgg ggcgacgctc gtgcttgacg caggcgcgcg taagagcctg
     2641 ctcgacgcgc ggttcgacgt tacattcggg ttggcgacag acggcagcga gttcagagac
     2701 gaccagatag cgggcgcgtt ccagcatccg ctgttcgcgg aaactgagcg gcttcgaatg
     2761 gctgagatag gtcaggtgct tgagcacgtc ggcgacttcg aagatgtcgc ccgtgcgcat
     2821 cttttcggaa aattctttga agcgatcttt ccagtcctgt ggcgcggcgg caaagtcgtc
     2881 gcccagattc ttcaacagca tttcacattc ggcggaagag atcggggaac gcaagcccac
     2941 ttcgcttgcg ttggcgacgg ggacgagtac gactgagttg gtggctgcga ggcgaagagt
     3001 ataaaagggg atagctattg cgcctatctg tttctgctcg acctgttcga tggttccgat
     3061 gccatgattg ggatagacga ctttctgacc gattttaaag cccaccacac acctcctaac
     3121 actgtctgcg aaaaattttt cactgcgtga acagtggccg ggttttgtag gtttaccctc
     3181 ttgctcttgc ggcgcaggac gcgctaaagg catctgctta aaacgtcaca aaagtgtaac
     3241 agtcggtctt gcgaagcgtc aagagcgagg atgagcgggg gcgtcggagg atgaattaaa
     3301 gcgtctgcct ttcattcatc atacggtagt ctcgtttcgg ccaagaccac tatggagcag
     3361 cgagttgtga atgtcgagcc ctggccggga acgaatggca agtgacaact gacgaccgtt
     3421 aagcgacgag cgatgtgtga cctgtgacaa gactgtggaa agtaagtttc gctacctgaa
     3481 tcgtctcagg tgagacgatc aaggaagcgg atgaagagga atgagagagc cgcgatgagc
     3541 gccgcaatgg ggatcgtcag cacccacgcc cagacgatgc gacccgccac gccccacttg
     3601 acggcggaga ggcggcgcgt cgcgccgacg ccgacaatcg cgcccgtgat ggtgtgcgtg
     3661 gtggagacgg ggatgcccgc gagcgtcgcg ccgaagagcg tcaaggctcc ggctgtctcg
     3721 gcgcagaagc cgcccacggg tttgagcttc gtgatcttcg atcccatcgt gtgcacgatg
     3781 cgccagccgc cggagagcgt cccggcggca atcgcggcgt gcgccgtcag gacgacccag
     3841 agcggaattt cgggaagttt gccgccctcg cccgcctgga ggaggttgct cgtcacaagg
     3901 acgatggtga tgatgcccat cgtcttttgc gcgtcgttgc cgccgtgtcc gagcgagtag
     3961 gcggcggcgg agagcagttg gccgcgacgg aaaagtttgt ccacgcgcgt cggcgtcgaa
     4021 cggcggaaca tccagaagac ggcgaccatc atgaagaagc cgagcgacat gccgatgagc
     4081 ggcgaggcaa tgatgaagac gagcgttttg atccagccct ccggcttcaa caacccctcg
     4141 aagccgccgt aagcagctat cgccgcgccc gcgtagccgc cgacgagcgc gtgcgaactc
     4201 gacgtgggga gggcgagata ccaggtgatc aagttccaga tgaccgcgcc gagcagcgcg
     4261 gcgagcagca cgtagaggcg catgtccgac tgaatcaatt cgatcctgac aaggtcgccg
     4321 ccgatggtct tggcgacgcg cgtgccgagg acggcgaagg cgatgaagtt gaagaacgcc
     4381 gcccacacga ccgccatgcc cggcgagagc acccgcgtgg aaaccaccgt cgcaatcgag
     4441 ttcgccgcat catgaaagcc gttgatgtag tcgaagacga gcgcgatgaa gatgatgaag
     4501 ataacgaagg ctaaggtgac agacatagga gcgaagaggg atgagggatg agggatgagg
     4561 gatgaatagg aaagcactca agagacagaa tgcttttctt catccctcat ccctcatccc
     4621 tcatcccttc ctttcaggcg tttttgatga tcacgctttc gatgatgtta gcgacctgtt
     4681 cgcagcggtc aacggcggct tcgagttttt cgtaaatctc tttgaacttg agcacggtga
     4741 gcgggtctgt ctcgcggcgg aacagttcgc cgatggctgc gtggtacgtg tcgtcggcga
     4801 cgttttcgag ccggtggatt tcgacgagac gttgattgat gccggtgggg cgcgcgagcg
     4861 tcgagacgat ttcgtggagt tgcacggcca tgcgctggat cacgtcggcg aaggcgcggg
     4921 cgtgcgacgt gctctcctga atattgaaca tgaccatcgc gcgggcggcg tcgtcgatga
     4981 gatcaaccac gtcgtcgagc gcggaggaga gcatgtagat gtcttcgcgg tcgaaggggg
     5041 tgataaaaga cttgttgagg ctggtggaga tggtatgcgt cagttcgtcg caggcgtgtt
     5101 cgatggtctt gatgcgctgc gcgtaggcgt tgtagtcgcg gctgctgctc gaatcgctca
     5161 tcatctcgac gaggattttc gaggcgtcga aaatatagga agtcatctgc gagaagaaag
     5221 agaaatattg atcctcgcgg ggcagaaaac tgaaacgcgc catcttttta aaccggctcc
     5281 attaagtggg atgcgaaaga agaagccgca ctatagacga agcggggcgc gctttacaag
     5341 gtgaacgcgg gctgaacgag cggtgaaatg cggaacgcgg aacgcggaac cgggcgcacc
     5401 attttgtcct tacttcatcg ttccgcgttc cgcgttccgc gtttcaccaa aaaatggggt
     5461 tgcaacaggc ttcggggaga agctcgctgc aacccctgtc cgcaattagc tggcgggaca
     5521 atgttccggt gggttgcgcc gactctaaac gcgcgatgct aaatcgaaat taacgtcaag
     5581 ttaagagttg atgaaatatc gtgcgcgcga gccggtttcg tcaaaaacga aagatggtgt
     5641 cgagttgcag acgcgaagtc gggcgattga gcgaaccggc gggcgtcgtg ccgaacactc
     5701 ccgccagccc gtgcggacgc tgggagacga ggccggtgag cgagagcgta acgcgcgggt
     5761 cgacggtgta gttgaagatc atgcggtgcg cgcgcacgtc ggaaggctgc aagatgtctg
     5821 agaagttgaa gggggtgagc acggcgtctt tctcgatgcg catgagggtg tagttgaaga
     5881 gaaggtcgcc gcgctcgcgc gctttgccca cctgaatctc cgcccagtag ccgttgcttt
     5941 cgtcgttcgg gacgaggcgg tttgcgccgc ccgcgcccgc caccacaacg tcgtgcgtct
     6001 gcgtgttggt cacgaagttg aagaggagcg tgacggggaa gcgtctgctg tgcgtgagtt
     6061 cgaggcgcgc gatgagatcg acgaggttga agccggacga gagccgcccg tcgcggttga
     6121 cggcgttggt gttggcgagg gagacgccga ggctgctgcc cgccacgagc aactcgcgcg
     6181 ggatggcggc aaagcccgtg acggtctgtg ccggagtctc gcccgttgca gggagcgcga
     6241 cggtgacggg aacctgtagc tggccgccga agaactgcgc gggcgtgatg aactgcgtgc
     6301 cggagaaata gaggtcggcg gcggagagcg tgagcgcagc ctgcttgccg agggcgagcc
     6361 gcgtgcgcgc ctgtgcgccg tagagcgcga ggtcgcgccc gccgcgcctc gaagcatcga
     6421 gactcgcgcg cccgtccgcg ccgaggacga aggttgactg gcgttcgagc atgggcagtt
     6481 gccacgccac gagcgtcaga ttcttgagca cgtcgccctt gaaatcgcgc gagtagcttt
     6541 cgttgatgcc ttcgggttgg atgtccgcgt cgaaggtcat ctcggtgaag agccagggcg
     6601 tctcgaactt tccgccttgc aggcgcaagc cgggaatccg cgcggggcga taggtgagat
     6661 aggcttgatc gagtgcgaac tgtttgcggt tgaagaaatc cgtgagcgtc tggttgctgc
     6721 tcgacacgtc tgcgagcgtg ccggtggaga agcgcaagcc ccagtcgaac tctttgccga
     6781 gttgcccgcg cagggcgaga cgcgcacgca ggcgcgcacg gtggcgcgtg ctcaactcgt
     6841 tgccggagat ggccggatta tccgcgttcg caagcgcgtt caactggccg aacgtgcctt
     6901 cgtagcgaag cctgaggtcg ccgctgaaag tgatggagcc gagttgacgt gcgagcgtct
     6961 cggtggtctt ttgcgcctgc gcttcgatgg cacggacgcg cgtgtcgatc ccttcggtct
     7021 gcgggttctt cgtgcccgcc tcctgcgccg ccggagggtc tgcgcgcagc gtctcggtcg
     7081 ccgccgctgc gttcgacgaa tagacagcct ctctgacgtt tgcgccgtta acattcgatg
     7141 ccgcgtctgg ttccgtccgc gcgatgcgtg cgagtagttc gttaaggagc cgcgtctgtt
     7201 ccgcgagcgt cgagcgcagt tgctcgatct ccgtgcgttg ctgctgcaat tgcctgcgca
     7261 cgtcgtccag agagaggtcg ccgggcgtcg tctgttgtgg cgcgtcggcg cgcgtcttct
     7321 gcgcgttcgg gtcggatgcg gcggcgtgcg cggcggcctg cgtttgcgcg aaggcggaag
     7381 gcgcaagcgc gacgaagagt gcggacgcga gcagtgcaca ccggacggga tgaatcatgg
     7441 aaactccttc ttggaaacga gaataagagc gaaacgggcg gaaacgatca gcactttcgg
     7501 acgcctgcat ttgaactccg gctgaaccgg agcatgcgcc gggataaagt gtttcacgcg
     7561 ccgacatcat aaagtaagtg cgcgaaaaat attaagagct tatttcaaga ctcctcaaga
     7621 ccggcagccg ccgctcccta agactaggag cagccgttcg ttccctgaag atttctttgc
     7681 gactttgcgc tcccggcgcg agtttgattt tccgccggag gcgcgaagcc gggaggccgg
     7741 gcgcaaaagt aagagagcag ttttggcgcg tgctccggca gcgagcgtcc ggcgcgtcag
     7801 gcggagaata gtattgttcc cgcgcgaggt gcgggggtga accgtgtccg gctgttgatg
     7861 cgaacggcgg aagtataagc gcgggaggtt aacaaggtct gaacaggatg ttacaaatct
     7921 gaaaacttgc cgtgcgtcga actgcgtggc tcttcgccgc acggcaagtt ccgtgtgtct
     7981 tggcggggct gtgctatgat gcttcccgcc cctttttaac aattcgttct ctgacctttc
     8041 ctaacaggct tcggttgcta tgagatcagg ctccttcatg caaaagacgc gggcgttgat
     8101 gggcttcgac gcgctacgca atctggtttc cgattcgatg gcgatagacc tcggctcggc
     8161 cagcacgatc atctccgtgc gcgggcgcgg cattgtcgtt gacgaacctt cggtcgtggc
     8221 cgtcagcaag acgacgggcg agatcgtcgc tatcggtcgt gaagcgcaca cgatgcaggg
     8281 gcgcgaggcg cgcgacgtga cgctcgtgat gccgatggtg gacggcgtcg tcgcagactt
     8341 cgagcgttcg caaaggatgc tcgatcattt cgtgcgcgag gcgcgcagcg gcatctcgca
     8401 tttcagtcgc cgcgcggtga tgagcgtcct ctccggcgtg acacacgtgg agcaacgcgc
     8461 gctcatggcg gcggcggaag cggcgcacat cgggcgcgtc tacatggtgg aagagggctt
     8521 ggcggcggcc atcggcgcag gcgtgtcggt cgaagaacag aaggcttcgg cggtggtgga
     8581 catcggcggc gcgacgacga acgtggccgt cgtcgcgctc ggcatgatcg tctacgcgca
     8641 ggccgaacgc gtcggcagtt ccgatatcga cgaagcgatc atggatcggc tgcgtcgtca
     8701 tcacggcctc atcatcggcg cgccgaccgc cgaacgcttg aagatcgcac tcggctccgc
     8761 cgtcgaaccg aaagaccctc tgcgcacgct gaccgtcaaa gggcgcgacg tgcagacggg
     8821 cacgccgcgt gcgatcgaag tgacgagcga cgaagtgtgc agcgcggcgc agaacgtggt
     8881 gcagcgaatc gtgcgcgtgg tgcagcgcgg cgtggccgaa ttgtcgcctg aagtggcggc
     8941 ggacatctat gatcgcgggt tgatgttgac gggcggcggc gcattgctcg acggcatggc
     9001 cgccttcctg caatccgaga cgcgccttgt gacgcgcatg gccgaagacc cgcgccacgc
     9061 catcgtccac ggcctctccc aactcttcga cgacccgctc ctgctgcgtc gcgtcgcccg
     9121 caacgaaccc tcgctcctct tggacgcaga cgcgggcgcg tttgagacat agttccgcgt
     9181 gcgactgtta aaagtgatgc gctctcaaat cgaaatcgcc atcgccgccg ccctcagcgt
     9241 cgatctaccg ctctactgga tgtttacacg gcgcgattcg ctgggcaggc cgaatcctct
     9301 gacatccggc gaagccgccg ttttattgct ggtcttggcc tgctacctgt cggttcctta
     9361 catggtcgga agttttagac ggcggcgcgt gctgcgagcc gtcccgcttt ggttttgggt
     9421 ttctgtctta ggagccgctc tgatattgac cgccgattat tccatcggaa taagatacgg
     9481 actcagcaca tgggagggag tggggaaaga ttggtttgcg ttgaccgtct ttaccttgcc
     9541 cgttaccgcg ctggtttatt attccggggc gattgtcggc ctgattaaag aatggcattt
     9601 cgaccggaga gataatttaa ggatcgtccg acgctaaacg tgggaacaaa agccacgcct
     9661 cctgtgtctt aaaactcggc atacgtcacc tgtcacacgt cactgctttt tatgtctctt
     9721 acgcacattc ttcaccggca actacgcgtg gcgcgcgtct atggcatccc ggtcaggata
     9781 gattatcgct ggttcatggt cttcgccctg agcacgtggc tcattgcgag caacttcgag
     9841 cagggcacgc cgctcgtccg ctacgtcaac gtcacgcctt cgaccgcgtg ggtcgtcggg
     9901 ctggtgacga cgctcggcct cttcctctcg atcttcgggc acgaactgtc gcacgcgctc
     9961 gtcgggcgca tcgagggcat cgagacggag gagatcgtcc tgcacccgtt cggcggcatg
    10021 gcgcggctta gtcgtgagcc ggacaatgcg cgtgcggagt ttcgcatcgc ggtggcgggt
    10081 ccggcgtcga gttttctctt ctccgtggcg gcgttcggcg cgatgatgct ggccgcagcc
    10141 ttcgagcaga ggttggcggg cgcggtcttc ttcatcatcg gcttctggaa cctgatgctc
    10201 gccgtcttca acctgctgcc cggctacccg ctcgacggcg ggcgcgtgct gcgcgctttt
    10261 ctctggcacc gcaacggcaa actcgaagag gccacgcgca tcgcttcgct cggcgggcaa
    10321 ctcatcgcgt ggacgctctt cgtcttcgcg ctgcttctct atttcaagac gggcgacctg
    10381 ttcatggcct tgtggtcggt ggtcgtcgcg ctcttcctcc atagcgcggc gcgcagcgtc
    10441 ggcaccggac gaaaatatct ggagacggtg ggcgaggcga tgagcgcgcc catcgcggtc
    10501 gaaccggacg tgctcgtcag ccatttcctc gacctgtttt tgccgcagca cccgcaagag
    10561 gcttttcccg tcgcacgcgg gcggcggctg cacggcatcc tgacgctcgc ggacgtgcga
    10621 aacctccccg aagcgagcct tcaccgcacg cgcgtcgcag acgtgatgcg acccgtcgcg
    10681 cccgagttat tcgtcgatcc cttgacgccg ctgtcgcgcg cagacgccct catgaaacag
    10741 aacggcgcgg gcgcgctcgc cgtgctcgac ggcgcgggcg aacttatcgg cttcctccat
    10801 cgcggtcgtc tcaaacggcg caaagaaacc gaataattct tcgatttttc gcggcacacg
    10861 ctcgctcgcg tcctcttaaa cgaggcgagc gcagaggtcg gttgttgctt tgtgtcgagc
    10921 ttttcgagat aatcggcgcg gatgcgtgta acttaaactc tccagagagg actatagatg
    10981 ctggctgttc tcggcgcgct catcgcgaac gggctgacta ctatctcgaa gtttatcgcg
    11041 gcgctaatca caggctttgc gggcatgatg gccgagtcgc cgcactcgct cgcaggcacg
    11101 acgaatcagg tcttttcgcc gcccggcctg cgtctctacc agcgtccggc actcgcctca
    11161 acacctcgtt ttgatcaacg cgcacgccaa ctttcgcaac gagttggcga cggacgacat
    11221 cgagcaggcc gaaccgaaag gcgatatgat ctttttagag actgcgcggg aacgcaccgc
    11281 ccaacagagc acgccgctcc cgcagcagat cggatgaaag gaaccgtcag gtgacgagcg
    11341 agaaggggaa ggccgtgagt cgtaaataga aaagacgggt tgcatctatt cacgattgac
    11401 gattattgac gattcacgat ctactctctt caactcgtta cttgttactc gtcactcgtc
    11461 ttatgttttt cgcaccgacc tacattgagt atttagacag gctacgcgag ccgcgcaacc
    11521 ttgtggacat cgcgctggtc tttctgatcg tgtacacggt gctgaaactt ttgcgcggca
    11581 cgcgcgccgt gccgatggcc gtcggcatcg ccttcttcgc gctgctctac tggatcgctt
    11641 acaatcgcga tctcaccacg ctcgaattcg tcctgcgccc cgcgccgctc tatctcggga
    11701 tcgcgatcat cgtcctcttc cagacggaga tcaggcaggc tttgatctcg ctcggcaatc
    11761 agattcgcct gccggggctg gcgcgccatc gcggccagtt cggcgaaggc gtctatgacg
    11821 agatcgtgct cgcggcgacg acgctggctt cgaccaagac gggcgcgctc atcgtcatcg
    11881 aacgcggcgt cggtttgaag aacgtcacgg acggcggcgt gaaaatggac gccgaactca
    11941 gttacgatct gctcgtcacg atcttcaacc cggccacgcc cctgcacgac ggcgcggtcg
    12001 tcatccgtcg tcaccgcgtc gcggcagcgg cctgtttttt gccgctcacc ctgaacccgc
    12061 gcctctcgaa agacctcggc accagacatc gcgccgccat cggcatcacg gaagacacgg
    12121 acgccgtcgc ggtcgtcgtc tccgaagaga caggactcat ctctttcgtc atcggcgggc
    12181 aaatacgtcg cggactcgat gcgaccaagc tgcgcgcagc gatcttcgac gccatcaaaa
    12241 ctccggcgcg cacggcgaac gaagagcctg caaccctgtc agccgacgcc gaaagcgagc
    12301 gcaacgaagt tgtctcaacc tgaaccacgt aggccagcgg ttagaggccg gttaaagaga
    12361 ataagaaccg atctcttcca gcctccagct tctgacttct gacttctgct cttatgactt
    12421 tacgcgaagt tgaaagagat gccgcgcaga tggcgcgcag gtggctgaac gatctcctgt
    12481 ttgccgactg gggattgaaa ttgctcgcgc tcgccatcac gctcggtttg tggttcgcag
    12541 tgacggggca gcgcgtgccg accacggcgc gcctgcgcaa agtgccgctc tttttcgtcc
    12601 tgccgaacga gatggagatc agcaacgagc cgcgtgatga agttgacctg acgctgcgcg
    12661 gtaacaagcg cgtgctcgac gcgatcaaag ccagcgacct gtcgctcagc tacgacgcca
    12721 gcagttacag gccgggcgag cgcgtcgtgc aactctcgcc caataacatc acgctgcaac
    12781 tgcccgtagg cacgagcgcg gaagatgtca ccatcgaacg catcgaaccg gggagcgttc
    12841 cgctcaggtt ggaacgtcgc atcgagcgcg agcttgaagt tgaacctcgt ctggaaggca
    12901 aactgccgga aggcttcgag ttgacgggca cgcaagtcct cccggcgcgc gtgcgcgtgc
    12961 gtggtcctga gagccacgtc aacgcgctcg aacgcgcgtc caccgaaacc atcctgctcg
    13021 aagggcgcag ggagactttt accgccccgc aaacagccat cgacatacaa gaccgcaaac
    13081 tcgtgacgct cgatgcggta gttgacgtga tcgtgaaaat ctccgagacg caggcaacga
    13141 agcgtctggc gggcgtcgcc gcgcgcacga ttgacggctc ggatacgcgg ctcacgattg
    13201 aagtgcgcgg cgcacgctcc gtcgtcgaac ctttgcgcgc ctcagacctc tcggtcgtct
    13261 tcgacacggc agccgacggc acgcagaagc cgcgcctcgt gctcccctca catctggaag
    13321 gccgcgtcga attaatctca accaatcaat aggaacgttg aactgtgaac tgctggcagg
    13381 aaataacagc cgacggccaa cagttaacag ttaatcgcca acagcttttt tatgcacaaa
    13441 cttttcggca cggacggcat gcgcggtgag gccggacgct tcccgctcga cgcgccaacc
    13501 atcgaaaccg taggacgctc gctcgcgcgc caactggcgg aatgcgccgg aggcgaaggc
    13561 cgcgcgccgc gcatcatcgt aggacgcgac acgcgcgaat ccggcgagtg gatcgagcgc
    13621 gcttttctct atggcgcgcg tgcggcgggt gcgtcgtgcg agtcggcagg catcatcacg
    13681 acgccgggcg tcgccttcct cacgcgcacg cttccggctg aagcgggcgt cgtcatcagc
    13741 gcgtcgcaca atccttacca cgacaacggc atcaaaattt tcgccccgac cgggcgcaaa
    13801 ctcgacgacg ccaccgaacg gcgcatcgaa gccgacatcc ggcaagcgca cggcgaaggg
    13861 cgcgacccac gcgtggaagc gcgcgccaaa cgcgacgaag cggacgcgaa tggacaacgc
    13921 accggagcaa gcacgccgcg aagtgtcggt ggcgcaggag acgcagccca acgcgcggca
    13981 gctttgcagg cgcgctatct ggattacctg tcggaagagg ttgcgccgga tttgcgtctc
    14041 gataatctgc ggctcgttct ggactgtgcc aacggcgcgg cctcgcagtt cgcgcccgcg
    14101 ctcttcgaac gcctcggcgc agaagtgttc gccacggggc agacgcccga cgggcgaaac
    14161 attaatcacg aatgcggctc gctctacgtc gaacatttgc gcgaacaggt gttgacgaac
    14221 cacgctcatc tcggcgtcgc gtttgatggc gacgccgacc gcgcgctctt tctggacgcg
    14281 cgcgggcagg tggttgacgg cgatgcgacg ctgtgggtca tggcgaaaag tttgcaggcg
    14341 cgcggcgaac tcgcgggcga gcagatcgtc gccaccgtga tgagcaacat cggtctggaa
    14401 ctcgcgctcg gttcgcgcga gatcaaaatg ctgcgcgcgg atgtgggcga caagtacgtg
    14461 ctcgaagagt tgctgcgcac gggcgcgtcc cttggcggcg agcaatccgg ccacatcatc
    14521 ttcccgcgcg tgagcctcgc cggagacggg atgatcacga cgcttttcct gctgcgcgcg
    14581 atgcaggaga gtggagagtc gctcgaagcc ttgacggcgg gcttcacgcg ctacccgcaa
    14641 gtgctcgtca atgtgcgcgt cgccgagaag cgaccatttg cggaagtcgc ggagatcgcc
    14701 gtggaagcgc gccggacgga ggccgcgctc gacgggcagg gacgcctcct gcttcgctat
    14761 tcgggcaccg aaccgctggc gagggtcatg atcgaagggc ggcatcagga agatatcgag
    14821 cgtctggcgc atgcgctggc ggcggtcatc gaacgcgcgc tcggctaggt aaaatattga
    14881 cgatcaggaa aactttacgc ggcggttgat gtaattatta ctcgcgagcg gtcgccggaa
    14941 agtttgcacg gacaaccggc gcgggctggg aaagtcgaaa agactaagta aaaaggcgtt
    15001 gagagaaaaa ttttttgaaa atatgcttgc atggcatgga cattgcgatg tatattgtgg
    15061 ggcaagagag acggttggtg gatcctccgt ctcagtggtt gctggggtga ggacagtagc
    15121 cacgaattag aaccggcgca gcgttcccca agttcgctgc gccggttctg cttttttatg
    15181 ccctctcaac ggcgcgcttc acccttcctg ccccggttca acggcgcgcg tgctaaaatc
    15241 gacgccgttc tgcgtagact cttttcggca attcaaattt tccggggaag cccgatgccc
    15301 gcacacggac gctcataaat gaagacgggc gactaccggc gcgactacgc ggcctacgcg
    15361 gctgcgcttg agcgcgcccg atacgactac cacacgggcg tcacgccgga actgcgtctc
    15421 acgccgattc gagatcgcta tgcagacctc tggacgcgcg acgccgttaa cgatctcacg
    15481 cgtgcccgcg aggaaatttc ccggcagttt gaaaccgagc gcgtcgcttg cgccgccctg
    15541 ctgcgtgctg cgcaactcgg ttacgtggag gcgcaggcca cggacgtaac gcgcgaactc
    15601 gcgcgctgtc gtgcgtccgc cgtcgtcgag tggaacggcg cgagcctcgc gccggaggat
    15661 gccccggacg cgctcgccct cgaacctgat gcggcgcgcc gccgcgaact ctccgcgcgc
    15721 tggttcgaag ccctgcgcgc ctgcaacgac ctccgcgccg cgcgcttcga cacgctccgc
    15781 gaagccgcgc gcacgctaaa tttcaactct tacttcgaat tcctgtcagg catccacgaa
    15841 gagtcgagcc ttgagcgtct gagcgcggcg gctgacaact ttctcgcgcg caccacttcc
    15901 gtctacaact cgacattgtt tgaatgggct gcgcgccatt tgccgcccga attttcgcgt
    15961 gctcccgcgt atgccgacag tctctttctc aggcggctct cgcacctcga tccgttcttc
    16021 cccgcgagcg aactgcgtgc gacgtacgag gcgacaatgc aggacttggg agttcgcgcc
    16081 gggcaacaac ataacctgtc aatcgaagcg accgggcata aaatcgggag catgctcgcc
    16141 gcgtgttatg ccttgcgccc gcctgaagag gtgcgtctgg ttcatcgaag cgagggcggc
    16201 gcgagcctct atcagatgtt tttcgcggcg gcgggaggcg cgcaagcatt cgcgtgggtt
    16261 tcgcgcgacc tcgccgcgcg ttatcccgaa ctcgtctacg cgccggacga gacgacgcgg
    16321 gcgggtttcc aacttctctt cgccgatctg ctcgcggatg cagcgtggct caccgcacac
    16381 agaaacgtcc gtccttcgtc ggcaggcgag atcgcgcgct ccttcgcgtt cgtcgagttg
    16441 caactcacgc ggcgcgccgc cgtccgtttg caacaacagc gaattctgga tcaagcacgc
    16501 gatccgcttg acgcacaact cgccgaaggc tacgcggtcg cgctcgaaga ggcgataaat
    16561 tttcgccccg acccgtcgct ctatctgcga gatttaattt ccgacgaccc ggaagagacg
    16621 cgcgcgagtg cgcgcctcca acccgccgtc tacctgcgcg cgcgcctctt cgccaccgcg
    16681 ctcggcgaat actttcggac gcgctacggt catcgctggt gggctgcccg cagagccgcg
    16741 gacgagttga tcgacctgtg gaacaccggc tcgcgctaca cggtcgaaga gttggcttcg
    16801 ctcgcggggc tgggcgactt aaacttcgac ttgctggccg acactacgga agcggctctc
    16861 agaggcgaat gacacaagaa aggcgagtca gcgtgcgtct tccggcgcgc gaaaattacg
    16921 agatcgagat cggcgcaaac gcccttcgcc gtctgggcga catcgtgcgg cgcgctctgc
    16981 cctccgaggc gcgccgcatc gccctcgtct ccaaccgtcg cgtcttcgaa ctctacggcg
    17041 cgggcgtgct gcgcagtctg cgcgcgggcg gctttcaagt ctcacactgg ctgatgggcg
    17101 acggcgagca gcataaaaat ttgagaaacg ccgaacgcgc gctggccttc ttttccgaga
    17161 gccgcctgga acgaagcgac gcggtcgtcg cgctcggggg cggagttgtg ggtgatctgg
    17221 cgggcttcgc cgccgccgtc tacttgcgcg gcatcgcgtt tctacaagtg ccgaccacgc
    17281 tcctcgcgca aattgatgct tccgtcggag gcaaaaccgc tgtcaacacg cgcgaaggga
    17341 agaacttgat cggagcgttt caccagccgc gcgccgtcgt catctcgacg gagactttgc
    17401 agacgctccc cagacgcgag atgaccgccg ggtggtgtga agcgatcaag cagggcgcgg
    17461 ttggagatcg tcgcctttat gaacagacgc gccgttttct ggtcgcggaa aatgagaacg
    17521 aaaaggcgcg cgggtcgaag agtgagcgcg gggcgaaaca gaggggcggg cgcgcggatg
    17581 aactcgccgc tctgatcgcg gcgcagtgtg ccttcaaagc gaagatcgtg gcgggcgacg
    17641 agcgcgaagc cgtgggacgc acggacgcgc gttcgcggcg catcctgaac ttcggccaca
    17701 cgtccgcgca cgcgctcgaa gccgtcacag cttacaggcg ttttcgtcat ggcgaagcgg
    17761 tcggctacgg gatgatcgta gcggctgaaa tttcacacag gctaggcatg ctcgacggtt
    17821 cggagttaga atctttacgc gcttgcgtcg ggctggccgg acgcttaccc cgcgcagacg
    17881 atctcgaaga gcgcgctctc ttgcgcgtga tcgcttccga taaaaaaagc gtcggcggtc
    17941 aggtacgctg ggtgctgctt gagggcatcg ggcgtgcgcg aatcgtcggc gggcaggaga
    18001 ttccgccgcg catcgtgcgc gccgcgcttc atgccgcgct ggtgcggcaa ccttgaaaac
    18061 agaaatgagg aaattgaaat gagtcaggtg cgagacgaaa atttcagagc gcgttggacg
    18121 accgagcgtg gcagctcgcg cttcaatttc ctgatcacgg tggttgtgat cgcgctcgtc
    18181 ggttacgccg cttatcaata tgtgcccgtc gcttatcagg cttcgcagtt gaaagtctcc
    18241 atgcaggaca cggtggacaa ggcggtcgtg acgagcaaga acgctgcgtg ggcggaggag
    18301 cagctgagaa ggagcctgcc gatctacggc gcgccgccga atacgctcat caccgtctcg
    18361 ccgcgcgaag cgcgcctcga agcgcacgtc caatacagcg tccctatccc gctcctcgtc
    18421 acgacctacg aatataagtt cgatcacacg acgcgcagcg cgtatctgat gtccggcgga
    18481 gggtgacgca gagcacacac gcgcacaggg aaaagggtca ggggtcaagg gtcaaggaaa
    18541 gaagagcgtc gcgcttcttc accttgacct ttgacccctg acccttttcc tgtgcgttat
    18601 gcgtttgcga cttttgcgcc gagtgaactt tgcagttgga cgagcgaagc gggcgcgctg
    18661 gcgagccatg tggtcgggca gacgccgcag cgttcgcagg tgggcacgct cggacagggc
    18721 gcagttgact tggcgatctg cgcgcgttcg tgttcgcgct gcatgaaggc gcgaccgagt
    18781 ccggcatcca cgatctccca cggcaaaaac tcttcgtaag tgcgcgtgcg gctggtgtga
    18841 aaagccggat cgaaattcgt ctcgcgcaag gccgcgccga gatcgccgcc gttctgcgcc
    18901 atcgcttcga tcacacggct gacggaacgg tcgcccgacg agaacaacgc ctgctcgtgc
    18961 gcgatgcgcg cagacatcgc gcgcacttcc acgttcggaa ttcgcgcgag ttgtttcgaa
    19021 acccacttca gacgccgctt gagttcgcgc tcctcacaga tcggctccca ctggaaaggc
    19081 gtgttcggct tcggcacaaa cccgttcagg gcggggatga tcttgccgac gcgcccgaag
    19141 cgtctgcccg cttcgagcat gcggtctttg atgcgttcga cgagccgcac catctctgcc
    19201 agatcttcgt cagtctccgt cggcaagccg accatcaggt agagcttgat cgtcagcatc
    19261 cctcgatcaa agaccgcgcc gcagatctct acgatctcgt cgttcgtcaa attcttgttg
    19321 atgactttgc gcaggcgatc cgaacccgtc tccggcgcga ccgcgatctg ttggtcgcgc
    19381 gattcgacga gcgcgtccag cagttcgtcc tcgatctggt cgagtcgcaa actggagacg
    19441 gagatgcgga agttcatctc gcgcaagccc tgcaagattt cgctgatctc cgggtgatcg
    19501 cagacggctg tagacaccag accgatcttg tctgttttgc cgcgccactc cgcagccttc
    19561 gccagcacgt cgcgcgcagg cacgacgcgc ggcggccagt agttgaagcc cgcccagcag
    19621 aagcggcagc cttgcgagca accgcgcgaa atctctatca ggaaacgctc gcccatctcc
    19681 gcttcgggtg cccacaccac cgtcgaaggc gcgaacactt cacgcgcgcg caaggattcg
    19741 gcgatctccg tgtcgccgcg ccgcatcgcg cgtttcaaac ttccttcttt cggattctcc
//
LOCUS       DASWNS010000322         7316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_167165, whole genome shotgun sequence.
ACCESSION   DASWNS010000322 DASWNS010000000
VERSION     DASWNS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167165"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VGB76_11260"
     CDS             complement(<1..271)
                     /locus_tag="VGB76_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099517.1"
                     /translation="MNDFQSPNQGFKHQQQAWPPQSADGSWGSLQAQTAAQASVAERM
                     GFIRKVYALFFVGTLFAIGGVALGMSFPPLLAYAAAHPWIMLFAML"
     gene            complement(443..1744)
                     /locus_tag="VGB76_11265"
     CDS             complement(443..1744)
                     /locus_tag="VGB76_11265"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY0099518.1"
                     /translation="MVLKKFLFALAFALLSVAPARMQTDMQKNQRPRAREVGLRVGVL
                     PTGAQNAITDVAGVRVGHTTIIRGESIRTGVTAILPHAGNLFREKVAGAVFVGNGFGK
                     LAGSTQVNELGEIETPIMLTSTLSVPRVADAVLDYMLALPENSDVRSVNPLVAETNDG
                     GLNDIRGRHITREDVFNAIRNARGGAVEEGAVGAGTGTIAFGFKGGIGTASRKLPPAL
                     GGYTVGALVQTNYGGVLTINGAPVGRELGKFYLKDELERAAAAVAPSRASDARGKVKE
                     HDAPHEGRTTAADALPGDTSADGSIIIVIATDAPVDARNLRRMASRAMLGLGRTGAAA
                     TNGSGDYVIAFSSAPEVRTRMNASGRPGELAAPRELKLLSNDAMSPLFLAVIEATEEA
                     IYNSLFRAQTLKGRGGLTIEALPLERTLDILRKYNALGVTP"
     gene            1884..5027
                     /locus_tag="VGB76_11270"
     CDS             1884..5027
                     /locus_tag="VGB76_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY0099519.1"
                     /translation="MKPGCSIPFWACALVCTAVLFVSVNAQRRTAATPAPAANAATAA
                     RNDEAYTAKIKEYTTEKYFLTELVDHLPASTSVPSPDKVLGYVVGTPNKLTYTKDIHR
                     YMRELERTSPRVRVFVAPEKSEEGREQLLVAVSDEANLQRLDRYKEITARLADPRRTT
                     DAEAKQLIGEGKAFYWASGSIHSTETGSPEMLMEMAYRLAVEESPFIQEIRRNLIVLI
                     TPTLEVDGRDRMVDLYNYRKANPGKNSPSLLYWGKYVAHDNNRDGLGMALALTRNMMR
                     TFLEYHPQVLHDLHESVPFLYTSTGTGPYNAWLDPIVIDEWHLLAYHEVEEMTKRGVP
                     GVWTHGFYDGWAPNYMFYVANGHNSIGRFYETFGNGGADTMDRTVAQQAQRDWYRPNP
                     PLPRVKWSLRNNVNLQQSAILLAMNFTANNRERFLNNFYLKSKRAVAKAANEGPAAYI
                     VPGDATRPVEAADMVNLLRLMGVEVHRATREFTLKDEARPTAAAASGAARGAEGRSGE
                     QPQAGGAEARERATGADAKPPERRYPAGSYIIRMDQPYSRMADMMLDTQYYNASDPRP
                     YDDTGWTLGAMRNVPTVRVKDRAVLSAPMELLTTDASVRGKLTGASNASVYVINHNTE
                     NTLATLRYRLQDVRVSVAESEFKAGAQNFNAGSFIIRTEGNPAGLRARLEAAVTELGL
                     TAVAFDRVPDVKTHDISTPRIAILHTWQNTQNEGWYRIEFDRLKIPYSYISEHTIRDT
                     PNLRERFDVIIFPPVGGTAQSIVNGIPRRGDPIPWKASSLTPNFGNSPDQSEDIRGGM
                     GLTGVANLQRFVSDGGLFITVQNTVRVPVEYGMTSGVTIESPRQLQAAGSIYNATFTD
                     RKSPISYGYGEQLPIYFNQAPLLQVATGAPGGGGGGAGGGAATGARPSGRGGVNDPDV
                     IQAMPQAAPPPPRTPARPGEEQITEEQRLQLGPFYVPLRERPRVVLRFAREERDLLVS
                     GMLANGSELANRAAIVDVPLGRGHILLFATNPMWRHQTQGEFFLLFNAALNYDNLGVG
                     LVEPPTAPARETRSEEQ"
     gene            5152..5934
                     /locus_tag="VGB76_11275"
     CDS             5152..5934
                     /locus_tag="VGB76_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099520.1"
                     /translation="MQNYPPPPGAPNYSPSGIPQGQAPKSKKPWIIGGVVGCLLLIII
                     VVLALGGLAYLGFKKAEEAAKSINVNAPAPRQAANTKQYVNSREGRTGKLSDNFVDFS
                     FYYPAGWELDDDPTPNFVRVERKLTDDDAGSFTQENFAVGWVSATGTAVDKQLLPKLA
                     AQFNTQFAAQFPNYKKVSEGETRIGVYDGYEFRFTAEKNNVEFWGRVAMLPPPSGQTK
                     GVALVMIASDRAPELSGVEDVGEKGELPVILEAFRCGSGKSR"
     gene            6121..7056
                     /locus_tag="VGB76_11280"
     CDS             6121..7056
                     /locus_tag="VGB76_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0099521.1"
                     /translation="MAGTSAHDEGGAATATTATGGLSPIWFVVIAVLLWSTGGLFIKW
                     GTLSDAELSGARALFAAATVMLLTRREGFRLNVVTAVAAVFYAALLYLFVRATLMTTA
                     ANAIFLQYTAPVYILLAEPFFFKEKYRAADFVVVAFCLAGMSLFFVGQLRPQDLEGNL
                     NALASGACFAVFFLLLRHPRAREVNRASSVIYGNLLLALLMLPAVVKGAEHFTPANIL
                     IAAYLGIVQLGVAYTLLTLGVARGVKSLDAGIIGYIEPVLNPVWVFLFLGERPSGWAL
                     LGGAIIIAAVASHTVWNARKKGANEKAATARGRTV"
BASE COUNT         1392 a         2377 c         2231 g         1316 t
ORIGIN      
        1 cgagcatcgc aaagagcatg atccaggggt gcgcggcggc atatgctaac agcggcggaa
       61 acgacatgcc gagcgcgacg ccgccgatgg caaacagcgt cccgacaaag aagagggcgt
      121 agactttgcg tatgaatccc atccgttcgg cgaccgaagc ctgtgccgcc gtctgcgcct
      181 gtaagctgcc ccaactgccg tccgcgctct gcggcggcca agcctgctgc tggtgtttga
      241 agccttgatt cggggattga aagtcgttca ttaaaaacct tccttcgtta aaaaatccgg
      301 ctgaaatttt acgcccacgc gcgccgtatg caaattaaga tgccgacgcc cgcgcgaaag
      361 ctgcatcaag cggaattata cggcatccgg tacgggaact tatagatcat ccgatgcagg
      421 agcgtgcggc tcgatggttg actcagggcg tgacgccgag cgcgttgtac ttgcgcagga
      481 tgtcgagcgt gcgttcgagc gggagtgctt cgatggtcaa cccgccgcgc cccttcaacg
      541 tctgcgcgcg aaagagcgag ttgtagatcg cctcttccgt cgcctcgatg acggcgagaa
      601 agagcggcga catcgcatcg ttcgacaaca acttcaactc gcgcggcgcg gcaagttcgc
      661 ccggcctgcc gctcgcgttc atgcgggtgc gaacttcggg cgcgctggaa aaggctatga
      721 cgtaatcgcc gctgccgttc gtcgctgcgg ctcccgtccg ccccagtccg agcatcgcgc
      781 gtgacgccat gcgccgcagg ttgcgcgcgt caacgggcgc atccgtcgcg atgacgatga
      841 tgatcgaacc gtccgccgaa gtgtctccgg gcaacgcgtc cgccgccgtc gtgcgccctt
      901 cgtgtggcgc gtcatgctct ttaactttcc cgcgtgcgtc gctcgcacgc gagggcgcga
      961 ccgctgccgc cgcgcgttcg agttcgtcct tgagataaaa cttgccgagt tcacgaccga
     1021 cgggcgcacc gttaattgtg agcacgccgc cgtagttcgt ctgcacgagc gcgccgaccg
     1081 tgtagccgcc gagcgcgggc ggcagctttc tcgaagcggt gccgatgccg cctttgaaac
     1141 cgaaggcgat ggtccccgtg cccgcgccga ccgcgccttc ttcgaccgcg ccgccgcgcg
     1201 cattcctgat cgcattgaac acgtcttcgc gcgtgatgtg ccgcccgcgt atgtcgttca
     1261 agccaccgtc gttcgtctct gcgacgagcg ggttgacgga acgcacatcg gagttttcgg
     1321 ggagcgcgag catgtagtcc agcacggcgt cggccacgcg cggcacgctc agagttgaag
     1381 tgagcatgat cggcgtctcg atctcgccga gttcgttcac ctgcgtcgag cctgcgagtt
     1441 tgccgaagcc gttgccgacg aagactgcgc ccgcgacctt ctcacgaaac aggttgcccg
     1501 cgtgcggcag gatggccgtg acgcccgtgc gaatgctttc gccgcgaatg atcgtcgtgt
     1561 gtccgacgcg gacgcccgcc acgtccgtga tcgcattttg cgcgcccgtc ggcaggacgc
     1621 cgacgcgaag gccgacctct ctggcgcgcg gcctctggtt cttctgcata tcggtttgca
     1681 tccgtgcggg cgcgacgctc agcagcgcga acgccagcgc gaagagaaat tttttcaaaa
     1741 ccatttttta actatccacg gtttcctctc gcgcagagag aaaaattatg agatttgtgc
     1801 ggcgatgatt tatcatccct ccgctttcat ttcaaattgg tcgtgccgca cctgaatttg
     1861 tctcttctca atggaggaat tcgatgaaac ccggatgttc catcccgttc tgggcgtgtg
     1921 ccctcgtctg caccgccgtg ctcttcgtct ccgtcaacgc gcagcgcaga accgccgcga
     1981 cgcctgcgcc tgcggccaac gccgcgacgg ctgcacgcaa cgacgaagcc tacaccgcga
     2041 agatcaaaga gtacaccacg gaaaaatatt ttctgaccga actcgtcgat cacctgcccg
     2101 cctcgacgag cgtgccctcg cccgacaagg tgctcggcta cgtcgtcggc acgccgaaca
     2161 agctgaccta cacgaaagac atacaccgct acatgcgcga gcttgaacgc acgagtccgc
     2221 gcgtgcgcgt cttcgtcgcg ccggaaaaat ccgaagaggg gcgcgagcag ttgctcgtcg
     2281 ccgtcagcga cgaagcgaac ctccagcggc tcgaccgtta caaagagatc accgcgcgac
     2341 tcgcagaccc gcgccgcacc actgacgcgg aagcgaaaca gttgatcggc gagggcaagg
     2401 ctttctactg ggcttcgggt tcgatccatt caaccgaaac cggctcgccc gaaatgttga
     2461 tggagatggc ctatcgcctc gccgtcgaag agtcgccgtt catacaggag atccgccgca
     2521 acctgatcgt gctcatcact cccacgctcg aagttgacgg gcgcgaccgc atggttgacc
     2581 tctataacta tcgcaaggcc aaccccggca aaaactcgcc ctcgctgctc tactggggca
     2641 agtacgtcgc gcacgacaac aatcgcgacg ggctgggcat ggcgctcgcc ctcacgcgca
     2701 acatgatgcg caccttcctc gaatatcacc cgcaggtctt gcacgacctg cacgagtccg
     2761 tgccctttct ctacacctcg acgggcaccg gaccatacaa cgcgtggctc gatccgattg
     2821 tcatagacga atggcacctg ctcgcctatc acgaagtgga agagatgacg aagcgcggcg
     2881 tgcccggcgt gtggacgcac ggcttctacg acggctgggc gccgaactac atgttctacg
     2941 tcgccaacgg ccacaactcc atcggtcgtt tctacgagac gttcggtaac ggcggcgcag
     3001 acacgatgga tcgcaccgtc gcgcagcagg cgcagcgcga ctggtacagg ccgaacccgc
     3061 cgctcccgcg cgtcaagtgg tcgctcagga acaacgtcaa cctgcaacag tcggccatcc
     3121 tgctcgccat gaatttcacg gccaacaacc gcgagcgatt cctcaataac ttttatctca
     3181 agagcaaacg cgccgtcgcc aaggccgcga acgaagggcc tgcggcctac atcgtgccgg
     3241 gcgacgcgac gcgccccgtc gaggcggcgg acatggtgaa cctgctgcgc ctgatgggcg
     3301 tcgaagtgca tcgcgcgacg cgcgagttta cgttgaaaga tgaggcgagg ccgacggcag
     3361 ccgccgcctc aggtgcagcg cgcggcgcgg aggggagaag cggcgagcag ccgcaagcgg
     3421 gcggcgcgga ggcgcgcgag cgcgcgacgg gcgcggacgc caagcccccg gagcgacgct
     3481 acccggcagg ctcttacatc atccgcatgg accagcccta ctcgcgcatg gccgacatga
     3541 tgctcgacac gcagtattac aacgcgagcg acccgcgccc ctacgacgac acgggctgga
     3601 cgctcggcgc gatgcgcaac gtgccgaccg tgcgcgtcaa agatcgcgcc gtgctctccg
     3661 cgccgatgga actgctgacg acggacgcga gcgtgcgcgg gaaattgacg ggcgcgtcga
     3721 acgcgagcgt ttacgtcatc aatcacaaca cggagaacac gctggcgacg ctgcgttatc
     3781 gtttgcagga cgtgcgcgtg agcgtcgcgg agagcgagtt caaagcgggc gcgcagaatt
     3841 tcaacgcggg ttcattcatc atcagaaccg agggaaaccc ggcgggcttg cgcgcccggc
     3901 tcgaagccgc cgtcacagag ttggggctga cggcggttgc gttcgaccgt gtgccggacg
     3961 ttaagacgca cgacatctcc acgccgcgca tcgccatcct gcacacgtgg cagaacacgc
     4021 agaacgaggg ctggtatcgc atcgagttcg accgcctgaa aatcccctat agctacatct
     4081 ccgagcacac catccgcgac acgccgaatc tgcgcgaacg cttcgacgtg atcatctttc
     4141 cgcccgtcgg cggcacggcg cagtcaatcg tgaacggcat cccgcgtcgc ggcgacccga
     4201 tcccttggaa ggcttcctcg ctcacgccga acttcggcaa ctcgcccgac cagtccgaag
     4261 acatacgcgg cggcatggga ctcacgggcg tcgccaacct gcaaaggttc gtctcggacg
     4321 gcggactctt catcactgtc cagaacaccg tgcgcgtccc cgtcgaatac ggcatgacga
     4381 gcggcgtgac gattgagtcg ccgcgccaac tgcaagccgc aggctcgatc tacaacgcca
     4441 cttttaccga ccgcaaaagc ccgatcagtt acggctacgg cgaacagctt ccgatctact
     4501 tcaatcaggc tccgctcctt caagtggcga ccggagcgcc gggcgggggc gggggcggcg
     4561 caggcggagg cgctgcgacg ggcgcgcgtc cttcgggacg cggcggtgtg aacgacccgg
     4621 acgtgataca ggcgatgccg caggccgcgc cgccgccgcc acgcacgccc gcgcgtcccg
     4681 gcgaggaaca gatcacggag gaacaacgcc tccaactcgg ccccttctac gttcccctgc
     4741 gcgaacgccc gcgcgtcgtg cttcgcttcg cgcgcgagga gcgcgatctg ctcgtgagcg
     4801 ggatgctggc aaacgggtcg gagttggcga atcgcgcggc catcgtggac gtgcctctgg
     4861 ggcgcggtca tatcctgttg ttcgccacca atcccatgtg gcgacaccag acgcagggcg
     4921 aattctttct cctcttcaac gccgccctca actacgacaa cctcggcgtc ggcctcgttg
     4981 agccgccgac cgcgcccgcg cgcgaaacaa gaagcgaaga acagtaaaag caaggatgag
     5041 ggatgaggga tgagggatga ataggaaaag gttcagcttg ataagcgggc tttgttttat
     5101 tcatcccaca ttccacatcc ctcatccctt aatttaaagg agactctaaa catgcagaac
     5161 tatcccccgc cgccgggtgc gccgaattat tctccgtcag gaataccgca gggacaggct
     5221 ccgaagtcga agaagccctg gatcatcggc ggcgtcgtcg gctgcctctt gctgatcatc
     5281 atcgtcgtgc tcgcgctcgg cgggctggct tatttaggtt tcaagaaggc ggaggaagcg
     5341 gcgaagtcta taaacgtcaa cgcgcccgcg ccgagacagg cggcgaacac gaagcagtac
     5401 gtgaactcgc gcgaaggccg caccggcaaa ctctcggaca acttcgtcga tttctctttc
     5461 tattatcccg ccgggtggga actcgacgac gacccgacgc ccaacttcgt cagggtcgaa
     5521 cgcaagttaa ccgacgacga cgcgggcagt ttcacgcagg agaacttcgc cgtcggctgg
     5581 gtgagcgcaa ccggcacggc ggttgataaa cagttgctgc cgaaactggc ggcgcagttc
     5641 aatacgcagt ttgcggcgca gttcccgaac tacaagaagg tgtcggaagg cgagacacgg
     5701 atcggcgtct acgacggcta cgagtttcgc ttcacggcgg agaagaacaa cgtggagttc
     5761 tgggggcgcg tggcgatgct gccgccgccg tccggacaga cgaagggcgt cgcgctcgtg
     5821 atgatcgcgt cagaccgcgc tccggaattg tcgggcgtcg aagacgtggg cgagaagggc
     5881 gagttgcccg tcatactcga agcgttccgc tgcggttcgg ggaagtccag atagcgtctc
     5941 ttatcgcagc ttgcagccgg gtgcgacctg agaattcaga ctcgccgcag aggcgcggag
     6001 ccgtgccgca gtcggagcgc agaggagaca tgcaactctc tctgcgcccc gcttgcgcca
     6061 actctccggc gtctctgcgg tgaatctttt catcgcctcc gcacgacgaa cgctataaaa
     6121 atggccggaa cttctgcgca cgatgagggt ggagcggcaa ccgcgacgac ggcgactggc
     6181 ggcctctcgc ccatctggtt cgtcgttatc gccgtgctgc tgtggagcac gggcgggctg
     6241 ttcatcaagt ggggcacgct ctcggacgcg gaactctccg gcgcgcgtgc gctcttcgcg
     6301 gcggcgaccg tgatgcttct gacgagacgc gagggctttc gcctgaacgt cgttacggca
     6361 gttgccgcag tcttctacgc cgcgctgctc tatctcttcg tccgcgccac gctgatgacg
     6421 accgccgcca acgcgatctt tttgcagtac accgcgcccg tctacatcct gctcgcggag
     6481 cctttcttct tcaaggagaa gtatcgcgcg gcggatttcg tcgtcgtcgc cttctgcctc
     6541 gcagggatgt cgctcttctt cgtcggtcaa ctcaggccgc aggacttgga aggaaatctg
     6601 aacgcgctcg cgtcgggcgc gtgcttcgcg gtcttcttcc tgctcctgcg ccacccgcgc
     6661 gcacgcgaag tcaaccgcgc ttcgtccgtc atctacggca acctcctgct cgcgctcttg
     6721 atgctgcccg ccgtcgtcaa gggcgcggag catttcacgc ccgccaacat cctcatcgcc
     6781 gcctatctcg gcatcgtcca actcggcgtc gcttacacgc tcctgacgct cggcgtggcg
     6841 cgcggcgtga aatcgctcga cgccgggatc atcggctaca tcgaacccgt cctcaatccc
     6901 gtctgggtct ttctcttcct cggcgaacgc ccctccgggt gggcgttgct cggcggcgcg
     6961 atcatcatcg ccgccgtcgc ctcacacacc gtctggaacg cgagaaaaaa gggtgcgaac
     7021 gagaaggccg cgacggcgcg cgggcgaact gtgtaagtct gcgagcaagc gtgtgcagac
     7081 gaagagagtt cttctgaaca cctgttgaca acggcgaccg aagttcaatc tcttcctttt
     7141 gaaccgttga aaattgaacc gttgaaaacg gctcttaagg agtcgcggtc ttggctgccg
     7201 attcgaggcg ttccacctcc gtcactgcga gcgggtattc gacgaagccc ctgttgccgt
     7261 gctctctgac ccagcgcagg tgcgggagcg cgtcgtcgcg tctgcctttg agggag
//
LOCUS       DASWNS010000323         5835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_246432, whole genome shotgun sequence.
ACCESSION   DASWNS010000323 DASWNS010000000
VERSION     DASWNS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246432"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1479
                     /gene="bshA"
                     /locus_tag="VGB76_11285"
     CDS             346..1479
                     /gene="bshA"
                     /locus_tag="VGB76_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEY0099522.1"
                     /translation="MNIGITVYPTYGGSGIVGSELGKELAKRGHTVHFIASALPTRLT
                     ELNERVRFHEVEMMSYPLFEHQPYTLALATKMATVAEMEQLDLLHVHYAIPHSISAIL
                     ARESLKPQRRLPVITTLHGTDITLVGADRSYLPITRYGIAQSDGVTAISHYLRRETEE
                     TFGYSQVEVIPNFVCGSEYTRQPSPSLRAELSPAGEPLLVHVSNFRPVKRPVDCVEIL
                     ARVRERGVGARLVMVGDGSERGRAEHRARCLNVEKFCTFVGKQPRIVDYLSVADVLLL
                     PSEQESFGLAALEAMACEVPVVASRVGGIPEVVTDGDTGCLSAVGDVDKMGADAAELL
                     TDEAARRAMGARARESALSRYNTDLVIPQYVEFYERILAASPL"
     gene            1448..2188
                     /locus_tag="VGB76_11290"
     CDS             1448..2188
                     /locus_tag="VGB76_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099523.1"
                     /translation="MSASSPHPPSKEEDASPLAVEASGSSPADERPIGYIIQNLERAY
                     GVPENRHRSDPLDMLIEIILSQATSDINSRRTFAALKRRFPTWDAALRARESTIARTI
                     QSGGLANQKAAVIKSLLRQIREERGALDLTFLHELAPGEAVRYLSQFRGIGPKTIACT
                     LLFACHTEVFPLDTHIFRILRRVGLIPQKCTDRRAHEIMNRLVPPGKFYSFHVNLIRH
                     GRALCRPRDPLCQRCPIVEYCDYGQTMI"
     gene            2348..2824
                     /locus_tag="VGB76_11295"
     CDS             2348..2824
                     /locus_tag="VGB76_11295"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY0099524.1"
                     /translation="MSYLIDGNNVMGQRVGWHRDRRRARRELLDELARFAARRKARVA
                     VVFDGAPDENFADGSSYRGVRVYYAARGSDADARIKSLVESSRERRTLLVVTSDRALA
                     DYVRRCGAQVVRAGEFRRRMEEAEAEGANEQTDAPPIEESTERWMRYFGVAPEDDE"
     gene            complement(2922..3869)
                     /locus_tag="VGB76_11300"
     CDS             complement(2922..3869)
                     /locus_tag="VGB76_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099525.1"
                     /translation="MKLTFDSSLRSLLLLLVASLAAQAQTPTPTPPAATEDIFVVDVK
                     AAKGRIEFGQPVNITNRAGYDNQPSFLPEGTSLLYTSQREGDQTDIYRYDFKTGQSAR
                     LTSTPEGEYSPTLMPGGKFFSVIRVEADKTQRLWKFPLAGGEPSLVLPNIKPVGYHLW
                     LDARTLALFILGAEGRPNTLQLVPTDISFLDTIHVNIGRALHLVPRRAAFSFVHKISP
                     DEWLVKIFDLKTHRSTTLTKTLPGSEDLAWTPDGTSILMAQDARLFQYNLAAPADGWQ
                     QVADFSAAGLKRITRLALSPGADRLALVAQNEDVRRVAK"
     gene            complement(4054..4491)
                     /locus_tag="VGB76_11305"
     CDS             complement(4054..4491)
                     /locus_tag="VGB76_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0099526.1"
                     /translation="MPEQAKNGDTVRVHYTGRLESGEIFDSSVGGDPFEFTVGAGDTI
                     AGFDEGVRGMEIGEQKKVEIEADNAYGQHIPARVQAIDRAIIKQSLGQEPEVGMALAM
                     QIAPDQQIPVTITEVTDTHVTLDANHPLAGQNLIFDIELVEIK"
     gene            4625..5002
                     /locus_tag="VGB76_11310"
     CDS             4625..5002
                     /locus_tag="VGB76_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099527.1"
                     /translation="MKKLASLAVTHALLISLLAPLAGAQQTRPAPASPAATAPPAPQQ
                     HARLDKAANRPQLTTHARLLRDGQLVYSGFSVPLQMTMYPDPKRIDIFGRLQLGSTLA
                     PGEYVLQVIVTDTLRNDKHRVAT"
     gene            5234..>5835
                     /gene="selB"
                     /locus_tag="VGB76_11315"
     CDS             5234..>5835
                     /gene="selB"
                     /locus_tag="VGB76_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEY0099528.1"
                     /translation="MTSIIVGTAGHIDHGKTTLVRALTGVDADRLPEEKRRGITIDLG
                     FAELSLGDVSVGFVDVPGHERFVKNMLAGAHGIDAVALVIAADEGVMPQTREHFDISK
                     LLGVSHGLVVITKTDIVDGELLELVRADAEELIAGSFLEHAPVVPVSARSGAGLDELK
                     ERLREVAALVPARSSVSVARLPVDRAFTMRGFGAVVTGTLV"
BASE COUNT         1112 a         1765 c         1773 g         1185 t
ORIGIN      
        1 ccgaatcaaa cgaaaactgc gttaatgttc acaaaaacgg gcgaaaaacg accggctgtt
       61 gggctttgtt gatgcctcgg gaaataaggg gcttgagggg acgaaaatat tttgacagaa
      121 ggattgtcaa aaaggctcga agggtttata tttaaattcc tcgcttgtcc cccttcacag
      181 gtgagacaag ttgagacgag aggcagagag tagcgagggc tttctgcctc ttgctatttt
      241 tagccgttag ctattaactg ttagccgtta gccggaaagg cagaccgctg ctgttactag
      301 ctaacggctt ttcttttttg acccggtttc agctaacagc tttctatgaa catcggcatc
      361 acggtctatc cgacctacgg cggcagcggc atcgtcggct cggaactggg caaggaactc
      421 gccaagcgcg ggcacaccgt ccacttcatc gcgtccgcgc tgccgacgcg tctcaccgag
      481 ttgaacgagc gcgtgcgttt tcatgaagtc gagatgatgt cgtacccgct cttcgaacac
      541 cagccctaca cgctcgcgct cgcgacgaag atggcgaccg tcgccgagat ggaacaactc
      601 gacctcctgc acgtccacta cgccatcccg cattccatca gcgccatcct cgcgcgcgag
      661 tcattgaagc cgcagaggcg tctgcccgtc atcaccactt tgcacggaac ggacatcacg
      721 ctcgtcggcg cggatcgttc atacctgccc atcacgcgct acggcatcgc gcaatccgac
      781 ggcgtgacgg cgatctctca ttatctcaga cgcgaaaccg aagagacgtt cggctactcg
      841 caggtcgaag tgattccaaa cttcgtctgc ggctccgagt acacgcgcca accctcgccg
      901 tccctgcgcg cagagttgtc gcccgcaggc gagccgctgc tggtgcacgt ctcgaatttt
      961 cgtccggtga agcgtccggt ggattgcgtg gagattctgg cgcgcgtgcg tgagcgcggc
     1021 gtgggcgcgc gcctcgtgat ggtcggcgac ggcagcgagc gcgggcgcgc ggagcatcgc
     1081 gcgcgttgtt tgaacgtgga gaagttctgc acgttcgtcg gcaagcaacc gcgcatcgtc
     1141 gattatctct ccgtcgcaga cgtgttgctg ttgccttcgg agcaggagtc gttcgggctg
     1201 gccgcgctcg aagcgatggc ctgcgaagtg ccggtcgtcg cctcgcgcgt cggcggcatc
     1261 cccgaagtgg tgactgatgg cgacaccgga tgcctgagcg cggtcggcga cgtggacaag
     1321 atgggcgcgg acgccgccga actgctcaca gacgaagcag cgcggcgcgc gatgggcgca
     1381 cgcgcacgcg agtctgcgct ctctcgttac aacacagacc tcgtcatccc gcaatacgtc
     1441 gaattttatg agcgcatcct cgccgcatcc cccctctaaa gaagaggacg cctcgccgct
     1501 ggccgttgaa gcgtccgggt cgtcgccggc tgatgagcga ccgatcggct acatcattca
     1561 aaacctcgaa cgcgcttacg gcgtgccgga gaacaggcat cgctccgacc cgctcgacat
     1621 gctcatcgag atcatcctct cgcaagccac cagcgacatc aacagcaggc gcaccttcgc
     1681 cgccctcaag cgtcgcttcc cgacgtggga cgccgccctg cgcgcacgcg aatcaaccat
     1741 cgcgcgcacg attcaatccg gcggcctcgc caatcaaaaa gcggcggtca tcaaaagtct
     1801 gctccgtcaa atcagggagg agcgcggcgc gctcgacctc accttcctcc acgaactcgc
     1861 gcccggcgaa gcggttcgtt acctctcgca atttcgcggc atcggaccca agactatcgc
     1921 ctgcacgctc ctcttcgcct gccacacgga agtcttcccg ctcgacacgc acatctttcg
     1981 catcctccgc cgcgtcggcc tcattcccca aaaatgcacc gaccgtcgcg cgcacgagat
     2041 catgaaccga ctcgtccctc cggggaagtt ctactccttc cacgtcaacc tcatacgcca
     2101 cggacgcgcc ctctgccgcc cccgcgaccc cctctgccaa cgctgcccca tcgtcgaata
     2161 ctgtgactac gggcagacca tgatctagtg acaagtgaca ggcagaagcc ttgaatcgcg
     2221 aatcgtcaat cgtgagtcgt gaatagaagg aaactgtttt agtctattca cgattcacga
     2281 ttcatggttc acgattcacg agcgacgaat cacgtttttc caacttgtca cttgtcactg
     2341 ttctcttatg tcttacttga ttgacggcaa caacgtgatg gggcagcgcg tcgggtggca
     2401 cagggatcgg cggcgggcgc ggcgcgagtt gttggatgag ttggcgcgct tcgcggcacg
     2461 gaggaaggcg cgggtggcgg tcgtattcga cggcgcgccg gatgagaatt tcgcggatgg
     2521 ttcgagttac aggggcgtgc gcgtctacta cgcggcgcgc gggtcggatg cggacgcgcg
     2581 gatcaaatct ctggtcgaat cgtcgcgcga gcgtcggacg ctgctcgtgg tgacttcgga
     2641 tcgcgcgctc gccgactacg tgcggcgttg cggcgcacag gtcgtccgcg ccggagagtt
     2701 tcgccgtcgc atggaggaag cggaagcgga gggcgcaaac gaacagacgg acgcgccgcc
     2761 gattgaagag agcacggaac gctggatgcg ctacttcggc gtcgcgccgg aagatgatga
     2821 atgagaaggg cgcaggtgca gccgcctgat gcccggatta agtcgcggtt gaggattcgg
     2881 tcaagccgga tcagcttttc aagacggttt cccggttcct gttatttagc gacccgacgc
     2941 acatcttcgt tttgcgcgac gagggcgagg cggtcggctc cggggctgag ggcgaggcgt
     3001 gtgatgcgtt tgagtccggc ggcggagaag tccgcgacct gttgccagcc gtcggcgggc
     3061 gcggcgaggt tgtactgaaa gagcctcgca tcctgcgcca tgaggatgct tgtgccgtcg
     3121 ggcgtccagg cgaggtcttc gctgccgggg agcgtcttgg tgagcgtcgt gctgcggtgc
     3181 gtcttcaggt cgaagatttt aacgagccat tcgtcggggg agattttgtg gacgaaactg
     3241 aacgccgcgc ggcgcgggac gaggtgcagc gcgcgaccga tgttgacatg aatggtgtcg
     3301 agaaaagaga tgtccgtggg gacgagttgg agtgtgtttg gtcggccttc cgcgccgagg
     3361 atgaagaggg cgagcgtgcg cgcgtcgagc cagaggtgat agccgacggg cttgatgttg
     3421 ggcagcacta aacttggctc gccgcccgcg agcggaaact tccagaggcg ttgcgtcttg
     3481 tcggcttcga cgcggatgac ggagaagaat ttgccgccgg gcatcagggt cggcgagtac
     3541 tcgccttccg gcgtggaggt gaggcgcgcg gactgcccgg tcttgaagtc gtagcggtag
     3601 atgtccgtct ggtcgccttc gcgctgcgag gtgtagagca ggctcgtgcc ttcggggagg
     3661 aaactcggct ggttatcgta gcccgcgcgg ttcgtgatgt tcacgggctg gccgaactca
     3721 atcctgcctt tggcggcctt tacgtcaacg acgaaaatgt cttccgtcgc ggcgggcggc
     3781 gtcggtgtgg gcgtctgcgc ctgtgcggcg agagacgcga cgagcagcag aagcagagag
     3841 cgaagtgaac tgtcgaaggt aagtttcatg gacggaaaat tttaaggaac gatgaaagat
     3901 gaatgatgaa ggagaattca gaacacagaa aatagaattc aggagtcaga atttagaatt
     3961 caggagtcag agtgaaactc ggcagatgtt ttcttctgac tcctgactcc tgcttttcat
     4021 tcatcgttca tcgttcatcg tttctaccgt gtgttatttg atctcgacga gttcgatgtc
     4081 gaagattaga ttctgcccgg cgagcgggtg gttggcgtcg agggtgacgt gggtgtcggt
     4141 gacttccgtg atggtgacgg ggatctgctg gtcgggcgca atctgcatcg cgagcgccat
     4201 gccgacttcc ggctcttgcc cgaggctctg cttgatgatg gcgcgatcaa tcgcctgtac
     4261 gcgcgccggg atgtgctgcc cgtaagcgtt gtcggcttcg atctccactt tcttttgctc
     4321 gcctatttcc atcccgcgca cgccttcgtc gaagcccgcg atggtgtctc ccgcgccgac
     4381 tgtaaactcg aaagggtcgc cgccgacgga cgagtcgaaa atctcgccgc tctctaaacg
     4441 ccccgtgtaa tgcacgcgca ccgtgtcgcc gtttttcgct tgctctggca taatgctctc
     4501 cgattcgttg gtttgtggtc gcggaagagc atcgcacact cacttgcgga aatccagcga
     4561 agcaacttgc ccgccgccga cgggcgaaca ttcaaacaaa cagcatcccg acggagtttc
     4621 acagatgaag aaacttgcct cgctcgccgt cacacacgcg cttctcatct cattgctcgc
     4681 cccgttagca ggcgcgcaac aaacgcgccc cgctcccgcg tcgcccgccg caaccgcgcc
     4741 gcccgcgcca caacagcacg cgcgtctcga caaggccgcc aaccgcccgc aactgaccac
     4801 gcacgcgcgc ctcttacgcg acggccaact cgtctacagc ggcttctccg tgccgctgca
     4861 aatgaccatg tatcccgacc cgaaacgcat agacatcttc ggtcgtctcc aactcggctc
     4921 cacgctcgcg cccggcgaat acgtcctcca ggtcatcgtc accgacaccc tgcgcaacga
     4981 caagcaccgc gtcgccacct agtgggtgga ttttgaaatc tacaagtgac aagtgacaag
     5041 tgacgagaga aagccttgaa ccgtaaagcg tgaagcgtga atagagaaga accgttctct
     5101 tctattcacg cttcacgatt tactgtcttc tcctctattc acgatttact gtcttcctcc
     5161 ctattcacga tttacgattc acgctccact gttttatctt gtcacctgtc actcgtcact
     5221 tgtcactgct cttatgactt ccatcatcgt cggcacagcc ggtcacattg atcacggaaa
     5281 gacgacgctc gtgcgcgcgc tgacgggcgt agatgcggat cgtctgcccg aagagaagcg
     5341 gcgcgggatc acgattgatc tgggctttgc cgaactctcg ctcggagacg tgagcgtcgg
     5401 cttcgtggat gtgcccggcc acgagcgttt cgtcaagaac atgctcgcgg gcgcacacgg
     5461 catagacgcc gtcgcgctcg tgatcgccgc cgacgaaggc gtgatgccgc agacgcgcga
     5521 gcacttcgac atcagcaaac tgctcggcgt ctcgcacggc ctcgtcgtca tcacaaagac
     5581 cgacatcgtg gacggcgagt tgctcgaact cgtgcgcgcc gacgcggaag agttgatcgc
     5641 cggatcgttt ctggaacatg cgcccgtcgt ccctgtgagc gcgcgcagcg gcgcgggact
     5701 cgacgagttg aaagagcgtc tgcgcgaagt ggccgccctt gtgcccgcgc gctcgtccgt
     5761 ctcggtcgcg cgtctccctg tagaccgcgc cttcacgatg cgcggcttcg gcgcggtggt
     5821 gacgggcacg ctcgt
//
LOCUS       DASWNS010000324        30346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_272156, whole genome shotgun sequence.
ACCESSION   DASWNS010000324 DASWNS010000000
VERSION     DASWNS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 30346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30346
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272156"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..2422)
                     /locus_tag="VGB76_11320"
     CDS             complement(92..2422)
                     /locus_tag="VGB76_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEY0099529.1"
                     /translation="MRRKTPHSFIRILFTCLVLALAGLLLNPTGTQAQTRAEYRAFWI
                     DTFNTTLNNHNDVVAIVNNAKAAKANAIFAQVRRRGDAWYLNSLEPRPDFTPIAADFD
                     PLQDLINEAHNNGIEVHAFVIMGAVWNKNPNFTPSATLGAPTNPNHVFNLHGGYDPTT
                     QKIIPGPNNWLTRTLLPDGAGGISFQGHRFGNDFWIDPGHPDAEAYTVNVLTHLVSNY
                     QLDGLHLDRIRYPEFTVPGQTPTAGTNIGYNATSVARFNTHYGRTGTPAQNDPQWNQW
                     RRDQVSNLVRRVYLNAIAIKPDLRVSAALIAFGGGPVTEAGWNSAEAYWRVYQDWRAW
                     TEEGILDIAMPMNYKREHTPSNVPLNDTWNEWLKNHQYNRAGMIGQGAFLNSIEGTLR
                     QARRALAPSAITGKSGIGVIFYSMGTSNVAVPADPFSVPPGKNSPVRSFAEFASGLTT
                     GKSVDGATLYEPAGLTPIFSEAATIPVLPWKSAPTLGHIMGFAKRADNTPLDTANVTI
                     KNLGTGAIRNTATDGGGFFGGVDLEPGEYLVKVEQGADVLYSCVATVTAGNVATADAA
                     IPETTAPVTNATLNPSTPDGSNDWYTSDVAVALDASDSCSGVAGTEYSTDGGASWQPY
                     AGSFEISQEGTNNILYRSTDRIGNVETTRSLTIKIDKTAPAIQLSATPSIINGKNGKP
                     VAVTLSGSGADAVSGLASVSYVVTDEYGTPLAIPARTLGGLTGEWTETLMLEARRNGN
                     DLDGRRYLVTATITDMAGHTATATTEILVSHDQRDR"
     gene            2628..3470
                     /locus_tag="VGB76_11325"
     CDS             2628..3470
                     /locus_tag="VGB76_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEY0099530.1"
                     /translation="MRSINRTDVNRTWLWIVLVIATCSLTTFAQTAAPAAQKQANNEY
                     QEGAILWTQTSGEARALAYQAFTLARMMLDRDLQSNRRSRMRRAIVVDADETVLDNSR
                     YQATLLKNRQNYDPLTWTAWVKRVEAEAIPGAVEFLRYAASRGVRVFYITNRNLSEKE
                     ATTANLKKLGFPDVSDETLLVRTDAQTSSKEVRRRSVSAKFRIVLLMGDNLNDFAEIF
                     QQSKTVADRLAAVERNKAEFGRRFIVLPNVMYGDWESALYDYNFKLTEEEKAARRKSQ
                     LRTY"
     gene            3552..4241
                     /locus_tag="VGB76_11330"
     CDS             3552..4241
                     /locus_tag="VGB76_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0099531.1"
                     /translation="MRVLLVEDERGAAKMLAKGLREESYAVDVAGDGETALEQAYLND
                     YDLIILDVMLPGKDGFEVCRELRASGSAVPILMLTARDAVEDRVEGLNTGADDYLVKP
                     FDFDELLARVRALLRRVPALLPEVIMVADLAINTRAQQVSRAGKPIELTTKEYALLEY
                     LARHANEVVSRADIAEHVWDESYDPFSKVIEVFIQRLRRKIDDERPVKLIRTRRGAGY
                     MLSTGDTDEDV"
     gene            4234..5649
                     /locus_tag="VGB76_11335"
     CDS             4234..5649
                     /locus_tag="VGB76_11335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEY0099532.1"
                     /translation="MFESVRARLTLWYVSALALILVVFGVAVYVMLSRALNRRVDEAL
                     RSTLEISITSLKHDTQEGQSSQSAAQSTAAELSHPQQALMIFDGAGRLLAEHPYEDDL
                     HIRLPDLGAIPADEVYLYTVAEENDADDHHRLAVRRVRIPPADTPYIILASQPLEAVE
                     DELESLREILYLATPAALLLAGLGGWFLARQGFAPVAAMARSARRIGAGSLDRQLPVV
                     NPRDELGQLATTFNELLARLNSAFVQQRRFMADASHELRTPLSVMSTAAGVTLKKPHR
                     EEEEYREALQMMAEQTRRLSRIVQDMFILARADAGHYPLQKRALYLNDLLEDIARAAG
                     LLAAHKDVTVELTNLPEATFHGDEDLLRQMMLNLLDNAIKFTPGGGVVRLGLTRRADE
                     YLLSVADTGTGIPAVAQAHIFERFYRADKARSRGEDGGAGLGLAIARWIAQAHEGELE
                     LDGSGHNGTTFIARLPAPARN"
     gene            5778..6221
                     /locus_tag="VGB76_11340"
     CDS             5778..6221
                     /locus_tag="VGB76_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099533.1"
                     /translation="MVLPVVVMFLTFGYAALAIVRSSAGELKQPLPPSLDNLAASRLV
                     EIRDVDGRVVLSGNFTLTTKRDGEIEGAAPLTASGVDPDAEGVAEIEISNEKNNVVEK
                     ELEIEVRNLAANSSYNLFIDGEQAATFKTNPRGVAELELTNQPSS"
     gene            6266..7243
                     /locus_tag="VGB76_11345"
     CDS             6266..7243
                     /locus_tag="VGB76_11345"
                     /inference="COORDINATES: protein motif:HMM:NF023695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HEY0099534.1"
                     /translation="MNRIVESHYYRKRPIALVLLLTLVAGSLLLLHSLRAGTPANTAA
                     EPAANVTAFNGGTFEASGVAHVPGTDGLLFVDDGRTDEVFWMRLGEGRSQAGAIKAVK
                     LGTSVVDLEGITTDGTHFYVVGSQSKPKGGDMAGLVRFRFDAQRQRVENAEAVSGLKR
                     FLSENVAELRGMGDRKYKDGGINVEGVAWDPREGRLLLGLRSPVVDGHALVVPLRLRD
                     PRRTFTADNVEVVDAKAIRLPLGGAGIRSIEYDERVKAFRVLSGAAANSEKADFKLWE
                     WNGDAARPVLREASTFDRRLKPEGVTRFSARGRDFTFIVFDTSAYSAID"
     gene            complement(7407..9239)
                     /locus_tag="VGB76_11350"
     CDS             complement(7407..9239)
                     /locus_tag="VGB76_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099535.1"
                     /translation="MAYAGASKKSASAYPLAADLPRGALLYAQFSDLPALVKRWHESK
                     LKERYLASTNFQQFQSRHLALKLVERWEEFNNALGFPLDSLAVGEAAENRAALAVYDI
                     GRLEMVFIAPLGEEKLAATKFFQSMDQFQATELPDGTVYYSRDVEADRGRRQQKFAFA
                     SLRGRLVVATGEPLLLRTLANIGGTAKNDRLSDDTAFKTLSAEVSPHFLTVWMNQTKL
                     NDDWYFKHYWVMRNVEQLKDIRASMLDLELRDDKWIEHRNFLLAGRKTAGSSSIPAAD
                     AQRIHTYIPGDAPYFKLRALDQGSDAASLVRDTFMDRMTDEGKKGVTNWKWQSYDDSD
                     FYPDNESEAAGDGGHYSLNPNFDSLIDDPVDARVEEKNELAHGRLRRETERKFVAGLS
                     ESIAAARPSYAATVASPHDSTGALFVEFRRGAVLTLQSPGNFSGQAFESAVGGLAESR
                     LMINGANAGLQWADALCGAQPCRLLALPMLGWTLGYAHRGAELVLANSPEMLQAMLDA
                     SSAARPPDANSALSFHDLTVIRFDRREQAFDSLMRKLDAPRVKAYWEERRKKEQVDSD
                     GASQEFFSGNIASLLDVASPVREIRIRRNLLPTRLREEVEIILK"
     gene            complement(9381..10331)
                     /locus_tag="VGB76_11355"
     CDS             complement(9381..10331)
                     /locus_tag="VGB76_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099536.1"
                     /translation="MRHWKSRLSALLIGFGIGFVLFLIVNFVMGFLRSHRQDAMRVEP
                     PGQDRCGAVDSFSTPEEILAALKSEDVSVRREVFRRLFLRPGVATTYYDYERDRDYPE
                     RAERAVLKYVNLDEQSGEEAVLTFVRLENPVALVLKKEECGWRLVGALGAWLRFEDYP
                     YEGWLEMPETVTRGVHEILLRESTGDATRYTRNVRLLKLIDGALAQVAEFSEESIEPV
                     EHYAEADWSMVKRRDTARYIPLAQTNGEKAKLRLETQAEVVKYSGSHVVHRYWLETDG
                     AWHVAGKHWRAREAARLRFIDKRTYEFVWDEQRKRFIEER"
     gene            complement(10420..18354)
                     /locus_tag="VGB76_11360"
     CDS             complement(10420..18354)
                     /locus_tag="VGB76_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099537.1"
                     /translation="MSKLKLTRRRLLTLTAISCISLTCAVVAQRLVTAEDKRGAAAPG
                     RVKDEKAEAIEAALFARTEFFGAQALVPYPTAEARNRLADVLKTYPDEPQITLRLAQL
                     DEKLGRFEEAERELEAFVQLQTGSPEALEILAAFHNRRAQFEKEAATIERMLAIAPAE
                     RRAQIFGRLVELARVHRLENYLKPGFYQKYIAQDEGVYGIIEQLLDKLSEEENYAEAL
                     KVLHQYKERFPERRNDLLEKEVSILVSMNRDAEAERVYLEAFDPFWPDGMSSRFYSFL
                     SRRDRFRAYGHELTGAFSRNPSDFDVAVRLIHYRNYTGEKSPRITAQLEQARAARRIS
                     WKPEELATLARILIADGDGDTASRFLYTLYVRNELNPGSPLRAKVLYQLFELLADARG
                     QRLALTRGDLKFYQDIATSDPHPGMLGGVLSLILSDTDPASELKDQDKRAVQYFNRAA
                     AFRIFTAYKQEYPTSTELAQMYLDIVRLYAAAKEPKIAEETLAEFEGRYGDAPQYAAV
                     ALKLADCYVALGRAEEERAAYQRIMDYLGGRRETGRPLVPAAGQSQTGAEGGGQQSAL
                     DIYSEPTETKPSLAAYPPQSNRGIDIPTESAEEGSTSPAYDNYSASPYTDHLTTVSFK
                     SQGHSVEAQGGEDVTYASILERHIASLAREKRTEAILSLYANELKKYPEEQGLYEQML
                     QWLGQTNLLEEQLKVHKQALQKFPTTLWRDRLARWFLRRERKQEFETFSRELLEQLSD
                     REIQEYLGKFIGEGTGANPSTFEARLYLGLYTLAHERFPHNQSFVRGLLKFYSAHGRW
                     GDWSRLMAEYYFVSSAIRDEFLSHLASRNGLRAHLDQARARCLAQTQAGGRVLDTLPY
                     KLFRADAAVRLSNYEEAIDAYRELNRLYPNTPEFSERLIAFTRSLGQRNPKFLEEAAS
                     AAHELAESSPASAAYRTRAGEIQAELGNYERARGEWEQLIGTGRGAPGTYLETATVYW
                     DYFQYADALRTIEQLRRQLNNPALYAFQAGAILEAEHRTTDALGEYVKALDFYSSDYD
                     EGLESDRAKRRLVKLYRREGIPAKLQQAFERERARRRDSSSLVLDYADLLSDAGEKRL
                     AAAHLKQEIARSDSREFLDSARNILDGDEDEGVGRLALKRLVAVAASPRFAISYRLRL
                     AADAEQHGRRQEAQAVLHELVGKFPTNYGVLNEAESFYWRMGLREESLRVLANAMQRG
                     KGRFYYIFGRKLAARLMLMNRLPAAREVLARLHAADKLNTEVFHELARVLIRTSQTDA
                     LKKAFAETLAAIREQDLDRKELHAETAELRKQMIDAFTRLKDYDAAIEQHIEIINREP
                     EDEGNVEAAINYAQRYGGADTLLAYYRRTSQEAYKNYRWNVVLARLYDARNDLPAAAQ
                     NYREAIANQPEMVELYDALAHVYTRMKNTGAALEALNRAAELTNDDPQYIRRLVEVLE
                     KAGRHGEAAEARRKLPDLAPKRETVGDRFADAARLRAVEKSKGVAAYRLAFDTFFADP
                     FAHQLKAGEITGYVQTVREEERLDQITGRLWQLRDKLIREVERKNSTRSGEARNSLQV
                     LDGALPEAVGALASQKGTGDELAALFADFNRRIEDALRNNDRYETLELLQNLSHRSGF
                     GVLEEKILNAQKDAAFRTGNQSFYHERLQRLVDFYTARGDYRQVIELLEAERAEGRDG
                     NDARYLRVVAENARLLGDSEKELQALRENYHRLIRHLPNQVNPTNELASRYLDALYEN
                     GEPGRQELRALAQQNSPYHLQLINFLLARGERELAHEAIERAPFSEAWKLSRHAGASL
                     ALGDYNSQSEGYFNAALQLKSIGELITRKPDASKQLIGDDWFQLAQSYGQWLGESARD
                     GAGANRAHALLVALVENRPRDPDEQTKLGRLLLERKEHKRALEHLSLALEMNPSDNNA
                     RAALGSAHFLAGDKARAGELWSEIIKGEQPGLAECELYLRTLRAHGLAAEAREKLFPL
                     VVAQVAVFNDHESDNEGANFYANTYGENRQKQLKSLRELIRALAVSFREGSDEVTSDE
                     VSAGEPREPQAEAARAKFFYRLSEAVSDNNLLPKMSIKESLISQGQLRPFYELLIKRS
                     PALSSYERDYDYTAQLDNTWSADEAEEALDQELGYKISEPPGERIRWQREYLDYLIAR
                     HETAAARRIISAVEAEVSRRYARPAWLRLMSLHLDVLDGQAEKALAGLRRFAGIEIAA
                     GRALINPPSIERLNMAVSMLRKEGRAAQADQLLEAAYTRELALERYEAHAFTGLARLA
                     FIRGDAARGLRLLQTMVALSGEETRGTAAADLASWPDIKVYAADRAIQDGAETENRLD
                     RIETLQLAAETAAAFTQFDAAINYRQQLFEASPEEETNRIELARLLVEKERYEEAVAQ
                     LAQIIGDRTASRRARWQAVYLAPEIIGVRYELRDALLERVRALNAGDEEMLAALSLSV
                     AGRTPESAKLFNALEAKNPNPYLNFFGALRERGQQDGGELKGFIHTLVAGKDETVVEA
                     FGFAEESPLRRIIRFYAMKGQPRAALLASRLDPTLKESEGTEQSGEAAADASNSTGDA
                     GQRTQFDAPESAPKDGTRYRTLRERAVERELESRRELLRLLSAAAEQVGDIDRAVEFE
                     TARLKLLPLMVERQSAQARIRQLLSLRQENSRRAVVTYSVDQSLIARR"
     gene            complement(18396..20846)
                     /locus_tag="VGB76_11365"
     CDS             complement(18396..20846)
                     /locus_tag="VGB76_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEY0099538.1"
                     /translation="MQRETTVQKRTGRRERAIIPALLLLALILVAGLPVRPGRAQSGA
                     LIPSTKEKPDASILSLQVMNVDVLIDNQHATVRVLQIYDNHTAQTLEGKYLFALPPAA
                     SVADFAVWDGDTRIPGVMMERRRANAVYGEIKQAAVDPGLLQQDDEHEGQTAFSAKVF
                     PINPYGSKRVEMEYTEMLAVESLNSHFTFPLKPSFGDAQRVGEFNLHIRVLGDYPITV
                     SDPGQKGYPLHVLKSQPGEFEAEFRAREIALTEDFSFDYRIDAPQSALSFIAYRAPER
                     ISAYDLRDPSLAARNPDGYFEARAIFNEGRSTQGDAAAGRATGESHAAPRNIMLLLDT
                     SLSMYGEKLTRAVEAVDYFLHGLSPADRFNLILFNEETNLFSPAPVEATPENVEHALQ
                     FIKGSSLGGGTDLRKVFEKALAATQSFPDGERNLVLISDANPTLGTTSLKTVARLFDD
                     EAKRRDKATHTRLYALALGSDANDALLAELAKKTHGYFAHARETEDISALLRILFAKV
                     STPGIENLRLTPADPSNFYQVYATGENSFDGSSFSFVGRYKKAQAQTALSLSGQYGTG
                     AINLSREVSLPEFEDTHQHLPRLWGRARVGKLLEEMNLNGEREDYIAEIIRLSQKYRF
                     VTPYTAFLAAPRALLRPRLIQPGDPVIRVKTDPSITSVFAVLPYGETLPLKFLQTEGV
                     WEGRFFAPAWMPDGTYKCRLLLTDRNGNGYEEEKSFVVDGHAPRLKVRLATATVRAGS
                     ELQLRVDADSDTARLVAKFYGAQPAQLFWSAQEKTNTGRLHVPVGLAAGQYTLTVTAE
                     DFAHNQSTTEVRVEVIER"
     gene            complement(20903..22960)
                     /locus_tag="VGB76_11370"
     CDS             complement(20903..22960)
                     /locus_tag="VGB76_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099539.1"
                     /translation="MRKITWQTGAVSPARRKLLLACLAGLCLFSFLSGVVWIRNQAQR
                     RLAQERARVAGQEFIPFEKSIRAPLARTEIKFWQNTESTRAVIRFQDSYFAATDGGMV
                     QFSTDGQFRRHYTVLDGLTESDLLSFAVFDSKLFIGTASQGLLSFDGERFESYRWLDR
                     EPQAITSMLADGGRLLCGTFGGGLIEFDGRRFTELKAGAERTRLLAVNCLFKDGSRLY
                     VGTFNDGLWLLEAGRWLHFRSADGLASNRVVGVAASGGNLFVASDFGLATATSASLSQ
                     THPSEQRRFHTLATLPTLSGLVSYGGLLLIVKDNGELLALNPQEAAARPSPPLRKIVW
                     PKPFDLSASRLMLLEERLWLLGSRGIWQTPEDASSETPAPLSLARFGHASESAPTLAS
                     NVISALAFDADGKFWAGNFRRGIDVLSGEGKKIAHLESEAVREINFLLPEGGGESAVL
                     AATSQGVVRFASQFRSTSTTKADGLPSNSIMHLSRAGGGDSAPAKAARGSELVLATGR
                     GLGVAQQGRWRALTTVQGLPSDQIYTTLFFNRSLFVGTLGGLAQVESGRVVRVFKDSN
                     SKLTHNWVTGLCAAGARLFIGTYGGGVFELAPSNELHGFTQEIGTPVVNPNAMWSDGQ
                     MLFVGTLGGAWVFNLHTQKWTHLKDELPARTVLSITGDAKYVYFGTTNGIARVEKSYL
                     TGI"
     gene            complement(23266..26451)
                     /locus_tag="VGB76_11375"
     CDS             complement(23266..26451)
                     /locus_tag="VGB76_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY0099540.1"
                     /translation="MRDPLLVICLLSGLSLGLACGFVSFAQSETDASRAETLNEDSSS
                     ARVKNLHQWGAISSFHGLPSDRIRAIAQDRAGVMWFGTDQGLARYDGRRVQAVVSEGL
                     PAGRVLALRVDGDGILWVGTESGAAQFLNDDFRRVAEIAGKAVNAIIAPEAGRVVLST
                     AQGLIFECRRNPDTTLSVKQLPRQTLLSADADQPGALGITSLAMYDGRLLAGTQSRGL
                     LALEGEEAKEIQSRPRAFFINALATAADGHLWIGARAKRGESGLYMADDPARPSKIDL
                     NTGAVMALGLDDRGDLWVGTGGRGAFRYRGTRQLDHFTFEGTAGGLRSDEIYVIFVDR
                     EGVVWFGTDRGVCRYDPRSPRIEDVSADKQSNFVRTLYQTRDGQLLCGTNRGLFIYDT
                     AAARWLPVQGLVNRTIYAIAEDASGKLLVGSSSGLYVNVNLKGRDDESYQHAIPAETE
                     ETEETDAQASPQQAVESVRAIRIFQGKTHVAVYGRGVERVEGEKRLLVWPVASPDARL
                     KNVTSLHTDGDDRLWIGTAENGVFIFDGRQATQDAALETLRGSPVWAIDGTRDGGLWF
                     ATARGLYSYRAGELRSVTPDTDVRSVVAENLGGTSALTGVWCATAGGGLLHIVADTEF
                     GTLVSRLDVEQGLPSQKAFAILPLRDGSGDGNAESLLIGTNRGVVRYSPGNTLPLLLP
                     TRILSRRLHQPEEMRAGINLDYPQNSLALEVAALSSRTFPEQFQYAFLLRDAAGRIIK
                     KKLSPDSQFLIENLRPGQYRVEVRAFSKDLLASAPLNFNFSVAKAPFPWTTVALSVLL
                     ALVLVALVWAIVEHQRIARASTALSRANRELASARLDLANEAERERRRIARDLHDQTL
                     ADLRHLLMLIDRLPAASAPQGNGHESSLDASVFRSEIEGVSNEIRRICEDLSPSVLEN
                     VGLTAAIEWALTNAASAHAAPGCKFEYEFVCDEELEERLDLAPAVQMQVYRIAQEVLN
                     NICRHASATRVRLAVANSADESFVMTIEDDGRGFDAHTRKGRKGRGLANIRARASLIE
                     ADVSWDKSPSGGTIFTLRKPRASQANAPLSG"
     gene            complement(26541..27314)
                     /locus_tag="VGB76_11380"
     CDS             complement(26541..27314)
                     /locus_tag="VGB76_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0099541.1"
                     /translation="MSILIAEDNVAQRRYLSDLLAREFPAHAPVIEAADGEAALELAL
                     KHRPELCVLDIQMPQLSGVKAARSIWREFPEARIIFWTQFPHEVYINEIRKIIRSADA
                     PPAYGFIHKNNPESRFLRFVAAVLEDGADMIDPAFKDSFKRPLLTEFEAEALYYLALG
                     LSNWAVARKCSLSLRGVESRLATLYEKLFVDVSEGTAQEAYDKLAYNVRTRAFFEALR
                     RGLINSDELEKAERELSNWIERDRQRYLEEQRGTGKGKQ"
     gene            27632..29545
                     /locus_tag="VGB76_11385"
     CDS             27632..29545
                     /locus_tag="VGB76_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY0099542.1"
                     /translation="MKDKRHAAETYAARDEDEIDAALERAARTAIGSQEGTVVVMDAQ
                     SGRLRSVINPQVAFAQAYAPGSAIKPFTALAALRAHLVDGQTRRFCRTRYAYRELTID
                     CSHQQSNVSFNLTQALAYSCNHFFGKISEGLETGSFDELLGSFGFGAPTGLKAMQAES
                     AGKLPRGEWRAGYALGESDQLLVTPIQLLTAYAALLNGGRLFVPGQAERVNFQTVERA
                     RLTIAPEHRSLIVEGMKGALRYGTAADAGLDTLPLDLFGKTGTSTRSNDYRSQGWFVG
                     FAADLDSDASARPESVSLAVLVFLKRARGADGARVARSIFAEYAKRRVEVERGRSEKQ
                     AVASNPAQLSAQRVRVHLVSQNITRDLSLEEYVMGVLAAEGSIESELEALKSLAVVSR
                     AYALHNLQRHASESFDLCNTTHCQRYLFTLMDERKHARFKNLLKRAVSETVGETLRDE
                     RGQIADAYFHAACGGMTSDIQTLWGVVGAPGYLSGVRDEYCVAARHSWVNVIPAAKLA
                     KALQGDSRSDVGARLENIVVANRDRTGRVEFIALEGENRRVLRGWDFKLIVGRALGWN
                     MLKSSRFDVTRAGQNFVFRGDGFGHGLGLCQAGAHALARRRGASYRQILRHYFPGTSI
                     GGREAPGLRRQET"
     gene            complement(29690..>30346)
                     /locus_tag="VGB76_11390"
     CDS             complement(29690..>30346)
                     /locus_tag="VGB76_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099543.1"
                     /translation="REALRIDAELEWAREGVVEVLKARNPVYRVMLRYFLWSSRLSSR
                     AMWGFIIGFYFLSRLIRTTLKSNPELGPILWPLYGLYLAFVLMTWIARPLFDLLLRLD
                     PMGRVVLSKKQITASNWVGGALLLAVVSLAAALLTGIPSFYLLAAGSGIMTMPIAGAF
                     NAETPRAKKVLPAYAVVLGALALLAFLVSLYNFETAMLIGIFFLLGFFVYTWVANLIK
                     "
BASE COUNT         5668 a         9149 c         9395 g         6134 t
ORIGIN      
        1 ggaaagagaa ccaatccctc tccgccacct ctccgaagtc aaacgcttca cggtgaagaa
       61 tactcgacac cgtgcgcgaa ccctctaact tttacctgtc cctttgatcg tgcgacacca
      121 gaatttcagt tgtcgccgtg gccgtgtgcc cggccatgtc ggtgatggtg gcagtcacaa
      181 gatagcgacg cccatccaaa tcatttccat tgcgccgcgc ctccagcatc agcgtctccg
      241 tccactcgcc ggttagacca ccgagcgtgc gcgccgggat agcgagcggc gtgccgtact
      301 cgtccgtcac gacatagctg acgctggcaa gaccggagac agcatccgcg ccgctgccgc
      361 tgagcgtgac ggcgaccggc tttccgttct ttccattaat gatcgaggga gtggccgaaa
      421 gctgaatggc cggagcagtc ttgtctatct tgatcgttaa actccgagtc gtttcgacat
      481 tcccgatgcg gtcggtggaa cgatagagaa tgttgttcgt cccctcctgg ctgatctcga
      541 agctgcccgc gtagggctgc cagcttgcgc cgccgtccgt cgaatattcc gtgcctgcca
      601 cgcctgagca tgaatcggaa gcgtcgagcg caaccgccac gtcgctcgtg taccaatcgt
      661 tcgagccgtc gggagtggaa ggattcagag tcgcgttcgt gacgggtgcg gtcgtctccg
      721 gtatagcggc gtcggcggtc gctacgttgc ccgccgtcac cgtcgcgaca caagagtaga
      781 gcacgtccgc gccctgctcg actttgacga gatattcacc cggctccaag tccaccccgc
      841 cgaagaagcc gccgccgtcg gtggccgtgt tgcgtatcgc gccggtgccg aggtttttaa
      901 tggtcacgtt cgcagtgtcg aggggagtgt tgtcggcgcg cttcgcaaat cccatgatgt
      961 ggccgagggt cggcgcagac ttccagggca gcacggggat ggtcgccgct tcgctgaaga
     1021 tgggcgtcag ccctgcgggt tcgtagagcg ttgcgccgtc aacggacttg cccgtcgtca
     1081 gacccgacgc gaactccgcg aaggagcgca cgggcgagtt tttccccggc ggaaccgaga
     1141 acgggtcggc gggaacggcg acgttcgaag ttcccatcga gtagaagatg acgccgatgc
     1201 cgctcttgcc cgtgatcgcc gaaggggcga gtgcgcggcg cgcctgcctg agcgtgcctt
     1261 cgatggaatt gagaaacgcg ccctgcccga tcatgcccgc gcggttgtac tggtgattct
     1321 tcaaccattc gttccacgtg tcgttgagcg gcacgtttga aggcgtgtgc tcacgtttgt
     1381 agttcatcgg catcgcgatg tcgaggatgc cctcctccgt ccacgcgcgc cagtcctgat
     1441 agacgcgcca gtaggcttcc gcagagttcc atccggcctc ggtcacgggt ccgccgccga
     1501 acgcgatgag cgcggcggag actctcaaat caggcttgat ggcgatggcg ttcagataga
     1561 cgcgccgcac cagattcgac acctgatcgc gccgccactg attccactgc gggtcgttct
     1621 gcgcgggcgt gcccgtccgc ccgtagtgcg tgttgaagcg tgccacgctc gtcgcgttgt
     1681 agccgatgtt tgtcccggcg gtgggcgtct gtccggggac ggtgaattcg gggtagcgga
     1741 tgcggtcgag atgcaggccg tcgagttggt aattgctgac gagatgcgtc agtacgttga
     1801 cggtgtaggc ttcggcgtcg gggtggccgg gatcgatcca gaagtcgttg ccgaagcggt
     1861 gaccctgaaa actgatgccg cccgcgccgt cgggcagcag ggtgcgcgtc agccagttgt
     1921 tcgggccggg gatgatcttc tgtgtggttg gatcgtagcc gccgtgaagg ttgaagacat
     1981 gattgggatt cgtcggcgcg ccgagcgtgg ccgaaggtgt gaagttcgga tttttgttcc
     2041 agaccgcgcc catgatgacg aaggcgtgga cttctatgcc gttgttgtgc gcctcgttaa
     2101 tcaaatcctg aagcggatcg aaatcggcgg cgatgggcgt gaagtccggg cgcggttcga
     2161 gcgaattcaa gtaccaggca tcgccgcgtc gtctgacctg cgcgaagatg gcgttggcct
     2221 tcgcggcctt cgcgttgttg acgatggcta cgacatcgtt gtgattgttg agcgtggtgt
     2281 tgaaggtgtc tatccagaag gcgcgatact ctgcgcgcgt ctgagcctgc gtgccggtag
     2341 gattcagcag cagcccggcc agagccagca ccagacaggt aaacaggatt cggataaagc
     2401 tatgaggggt ttttctacgc atctactaat cgtgcctcct taggaaggaa agggttgtgc
     2461 aacactggaa gtgaaccgcg tggcagatta ccacatgctt gcccctgcgc cgtagaatat
     2521 gggcgcgagt gtcggcacaa ctggaaatct tttgcgctcc cctttgctcg aacacgcccc
     2581 gccagtcgcc gggacatgga cacaaaattt taacggagag atgaaagatg agaagtatca
     2641 atcgcacgga tgtgaaccga acgtggctct ggatagtctt agtgatcgca acctgttcac
     2701 tgacgacttt tgcacagacc gccgctcccg ccgcgcaaaa gcaggcgaac aacgaatatc
     2761 aggaaggcgc aattctctgg acgcaaacat cgggcgaggc gcgcgcactg gcgtatcagg
     2821 ctttcaccct cgcgcgcatg atgctggatc gcgatctaca gtcgaaccgt cgcagcagga
     2881 tgcgcagggc gattgtcgtg gatgccgacg aaaccgtgct ggataacagt cgctatcagg
     2941 ccacgctcct gaaaaaccgg cagaactacg acccgctgac gtggactgcc tgggtgaagc
     3001 gcgtcgaggc cgaggccatc cccggcgcgg tcgagttcct ccgctatgca gcttcgcgcg
     3061 gcgtgcgcgt cttctacatc accaaccgta acctgtcgga gaaggaggcc acgaccgcca
     3121 acttgaagaa gctcggattc cctgacgtga gcgacgagac gttgctcgtc agaactgacg
     3181 ctcaaacttc cagcaaggaa gtccgccgcc ggtcggtgag cgcgaagttt cgcatcgtgc
     3241 ttttgatggg cgacaacctc aacgacttcg cagagatttt ccagcagagc aagacggtcg
     3301 cggacaggct ggcggcagtg gagcggaata aggccgagtt cggcaggcgg tttattgtcc
     3361 tgcccaacgt gatgtacggc gactgggaga gcgcgctcta cgactataac ttcaaactca
     3421 cggaagaaga gaaagccgcc aggcggaaga gtcagctaag aacttattga gcttcgagac
     3481 acgtgttaac agagtcctga acaacgggca agagccgcga gagaggccgc tcaagaggag
     3541 gagttgagac gttgcgcgtt ctactggtcg aagacgagcg gggtgccgcc aagatgctcg
     3601 ccaagggctt gcgcgaagag tcttacgcgg tggatgtcgc gggagacggc gagacggcgt
     3661 tggagcaggc ttacctcaac gattatgatc tgatcatcct cgatgtgatg ctgcccggca
     3721 aggacggctt cgaggtctgc cgggagttgc gcgcgagcgg ctccgccgtg ccgattctca
     3781 tgctcacggc gcgcgatgcc gtggaagata gagtggaagg actcaacacg ggcgcggacg
     3841 actatctggt caaaccgttc gatttcgacg agttgctcgc acgcgtgcgc gcgctgctgc
     3901 gaagggttcc agcccttctg ccggaggtca tcatggtcgc ggatttagcc atcaacacgc
     3961 gcgcgcaaca ggtatcccgc gcgggcaagc ccatcgaact cacgaccaaa gagtatgccc
     4021 tgctcgaata cctcgcgcgg cacgccaacg aagtcgtcag ccgcgccgac attgccgaac
     4081 acgtctggga cgagagctat gatcctttct cgaaagtcat agaggtcttc attcaaaggt
     4141 tacgccgcaa gatcgacgac gaacgccctg tcaaactgat tcgtacccgc agaggggcgg
     4201 gttatatgct ctcaacggga gatacggacg aagatgtttg agtctgtcag ggcacgtctg
     4261 accctctggt acgtgagcgc gctcgcgctc atcctcgtcg tgtttggcgt cgccgtttac
     4321 gtcatgctgt cgcgcgcgct caaccggcgc gtagacgaag ccctgcgttc gaccctcgaa
     4381 atctcgatca cctcgttgaa gcacgacacg caggaagggc agtcgagcca gagcgcggcg
     4441 cagagtacgg cagcggagtt gtcgcacccg cagcaagccc tgatgatctt cgacggcgcg
     4501 gggcgcttgc tcgccgagca cccttatgaa gacgacctgc acatccgcct ccccgacctc
     4561 ggcgcgatac ccgccgacga ggtctacctc tacactgtcg cggaagagaa cgacgccgac
     4621 gatcatcatc gcctcgccgt gcgcagggtg cgtatccccc cggcagatac accgtacatt
     4681 atcctcgcca gccagccgct cgaagcggtc gaagacgagt tggaatcgct ccgggagata
     4741 ctctatctgg cgacgcctgc ggcgctcctt ctcgccggac tcggcggctg gttcctcgcc
     4801 cgtcagggct ttgcgcccgt cgccgcgatg gctcgcagcg cacgccggat cggagccggg
     4861 agtctcgacc gccagttacc ggtcgtcaac ccgcgcgacg aactgggaca actcgccacc
     4921 actttcaacg aactgctcgc gcgtctcaac tcggccttcg tccagcagcg acgattcatg
     4981 gccgacgcct cgcacgaact gcgcaccccg ctctcggtga tgagcacggc ggcaggcgtg
     5041 actctgaaga agccgcaccg tgaggaggag gagtaccgcg aagcgctcca gatgatggcc
     5101 gagcagacgc ggcggctctc gcggatcgtg caggatatgt tcatcctcgc ccgcgccgac
     5161 gccgggcact accccctgca aaagagggcg ctctacttga acgacctgct cgaagacatc
     5221 gcgcgcgccg ccggactgct ggccgcccac aaggacgtga ccgtagaact gacgaatctg
     5281 cccgaggcca ccttccacgg cgacgaagac ctcctgcgcc agatgatgct caacctcttg
     5341 gacaacgcca tcaagttcac gcccgggggc ggcgtcgtcc gtctgggttt aacccgacgc
     5401 gcagacgagt atctgctttc ggtcgccgac accgggacgg gcatccccgc tgtagcgcag
     5461 gcgcacatct tcgagcggtt ctaccgcgcc gacaaagcgc gctcgcgtgg cgaagacggg
     5521 ggcgcaggct tggggcttgc catcgccagg tggatcgcgc aggcacatga gggcgaactt
     5581 gagttggacg gctccggcca caacggcacc actttcatcg cacgcctgcc cgctcccgcc
     5641 cgtaactgac tgtcaacacc ctacttctct cgtttaccgc ccgtttacca tagatttgat
     5701 acccttccct gataaattgc gagggcaaag agagttctgt caatgggggg tgaaatcgga
     5761 tgagttacag ggttaaaatg gttctgccgg ttgtcgtgat gttcttaact ttcgggtatg
     5821 ccgcgctggc gattgtccgg agcagcgcag gcgagttgaa acagccgctt ccgccgtcgc
     5881 tggataatct ggcggcgtcc aggctggtag agatcagaga cgttgacggt cgcgtcgtcc
     5941 tcagcggcaa ttttacgttg acgacgaagc gtgacggaga gatcgaaggg gcggcaccgt
     6001 taactgcgtc gggcgttgac ccggatgcag agggcgtggc cgagatcgaa atatcaaacg
     6061 agaagaataa cgtcgtcgaa aaagagcttg agatcgaggt gcgaaacctc gcggcgaata
     6121 gctcttacaa cctgtttatc gacggggagc aggcggcgac cttcaagacc aacccgcgcg
     6181 gcgtggcgga acttgagttg acaaaccagc cttccagttg aagcgccgga ttgttgttca
     6241 gagagtcttg aaccggaggt aagtcatgaa taggattgtc gaaagccatt attatcgaaa
     6301 acgtccaatc gcgctcgtcc tgctgctcac gctggtcgca ggatcgctgt tgctgctcca
     6361 ttcacttcgg gcgggcacac cggccaatac cgccgcagag cctgccgcga atgtcaccgc
     6421 atttaacggc ggcacattcg aggcgtcggg cgtggcgcat gtccccggca cggacggact
     6481 gctctttgtg gacgacgggc gcacggacga agttttctgg atgcggctcg gcgaggggcg
     6541 cagccaggca ggcgcgatca aggcggtcaa gctcggtacg agcgtcgtag acctcgaagg
     6601 catcaccacc gacggcacac acttctacgt cgtgggttca cagtcgaagc cgaagggcgg
     6661 tgacatggcc gggctggtgc gcttccgttt cgacgcacag cggcagcggg tggagaacgc
     6721 cgaggcggtg agcgggctga agcgtttcct ctcggagaac gtggccgaac tgcgcgggat
     6781 gggcgacagg aagtacaagg acggcggcat caacgtcgag ggcgtcgcgt gggacccgcg
     6841 tgagggaagg ctcctgttgg gcttgcgttc gccggtcgtg gacgggcacg cgctcgtcgt
     6901 gcccttgcgc ttacgcgacc cgcgccggac gttcaccgcc gacaacgtgg aggtggttga
     6961 cgcgaaggcc atcaggctgc cgctcggcgg cgcggggatt aggagcatcg aatacgacga
     7021 acgcgtgaag gcgttccgcg tcctgagcgg cgcggcggcc aacagcgaga aggccgattt
     7081 caagctctgg gagtggaacg gcgacgccgc ccgccccgtg ctccgcgagg cgagcacctt
     7141 cgaccgccga ctcaagcccg aaggcgtcac acgcttctcg gcgcgcgggc gagacttcac
     7201 cttcatcgtc ttcgatacga gcgcgtactc ggcgatagac taagaacttc tgttgcgact
     7261 ccgaaggtgc gtgagaggag gtagtgtcgc aaaaagtttc tatccacaca gggtgttgag
     7321 ttaacgccac actcttcact ctatgctgcc gttaattgcg gcggaaatat tttttgcgac
     7381 gctaccggaa accagccctg cgactcttat ttgaggatga tctcgacctc ttcacgtaaa
     7441 cgggtcggca gcaggtttct cctgattctg atctcgcgca ccggggaagc gacatcgagc
     7501 aggctggcga tgttgcccga aaagaactcc tgagatgccc cgtccgaatc aacctgttcc
     7561 ttctttcgac gctcttccca gtaagccttc acgcgcggag cgtccagttt gcgcatgagc
     7621 gaatcgaaag cctgttctct gcggtcgaag cgaatcacgg tcagatcatg aaacgataga
     7681 gcggaatttg cgtcgggagg gcgcgccgca ctgctggcgt cgagcatcgc ttgcagcatc
     7741 tccgggctgt ttgccagaac cagttccgcc cctctgtgcg cgtagcccag cgtccaaccg
     7801 agcatcggca gtgcgagaag cctgcacggc tgcgcgccgc acagagcatc cgcccactgc
     7861 aaacctgcgt tcgccccgtt aatcatcagg cggctttcgg caaggccgcc gactgcgctt
     7921 tcaaacgcct gcccgctgaa atttcccggc gactgtaaag ttaagactgc gcctcttctg
     7981 aactcgacga agagcgcgcc ggtagaatcg tgcgggctgg cgaccgtagc tgcataagac
     8041 gggcgcgcag cggcgataga ctcgctcaag cctgcgacaa atttcctctc cgtttctcgt
     8101 ctcagacgcc catgggctaa ttcgttcttc tcttcaactc tcgcatcaac cggatcatca
     8161 atcaaagaat cgaagttggg gttgagagaa taatggccgc cgtcgcccgc cgcttcgctt
     8221 tcgttgtccg gataaaaatc ggagtcgtca taggattgcc acttccagtt cgtgacgccc
     8281 tttttccctt catccgtcat gcgatccatg aaagtgtcgc gcacgagaga ggctgcgtcc
     8341 gagccttggt ctagagcgcg tagcttgaag tagggcgcgt cgccggggat gtaggtgtga
     8401 attctctgtg cgtctgcggc ggggatggac gaactcccgg ctgtctttcg accggcgagc
     8461 aaaaaattcc tgtgctcgat ccacttgtcg tctcgcaact caaggtcgag catggaggcg
     8521 cgtatatctt tcaactgctc gacattccgc atcacccagt aatgtttgaa ataccaatcg
     8581 tcgttgagct tcgtttggtt catccagacg gtcaggaagt gcggcgacac ttccgcgctc
     8641 agggttttga aggccgtgtc gtcggagagg cggtcgttct tcgccgtgcc gccgatattc
     8701 gccagcgtgc gcagaagcag aggctcgccc gtcgccacga cgagtcgccc gcgcagagag
     8761 gcaaaggcaa acttctgttg tcgtcgtccg cgatcagctt caacgtcgcg gctgtaatag
     8821 accgttccgt ccggcagttc cgtcgcctga aattgatcca tgctctggaa aaactttgtc
     8881 gccgccagct tctcttcacc gagcggcgcg atgaagacca tctccagccg cccgatgtcg
     8941 tagacggcca acgccgccct gttctccgcc gcttcgccca cggccagaga atcgagcgga
     9001 aagcccagcg cgttattgaa ctcctcccaa cgctcgacga gcttcaaggc aaggtggcga
     9061 ctttgaaact gttgaaaatt ggtgctcgca aggtatcgct ctttgagctt cgactcgtgc
     9121 cagcgtttga cgagcgcggg caggtcgctg aattgcgcgt acagcagcgc gccgcgcgga
     9181 aggtctgcgg ccagcgggta cgcgctcgca gactttttag acgctccggc gtaagccaac
     9241 agcgcggcac aaaccggaag cgcaatgaac gcgagcgcga aaagatgtcg ccgccgcaat
     9301 ctgcgtccaa acttaagggc aaacatatcc ttagcctttc atcaaacggc gcgagccgga
     9361 cagaacacac ggcctcccgc tcatctctct tcgataaatc tcttgcgctg ctcgtcccag
     9421 acaaattcgt acgtgcgttt atcaatgaac cgcaggcgcg cggcctctct cgctctccaa
     9481 tgctttccgg cgacatgcca tgcgccgtct gtctccaacc agtatcgatg aacgacgtgc
     9541 gagccggaat atttgacgac ctccgcctgc gtttcgagcc gcagctttgc cttctctccg
     9601 tttgtttgcg cgagtgggat gtagcgcgcc gtgtcgcgcc ttttgaccat gctccaatcc
     9661 gcttccgcgt aatgctcgac aggctcgatg ctctcctctg aaaattccgc gacctgcgcc
     9721 agcgcgccgt cgatcaactt caggaggcgc acgttgcgtg tatagcgcgt ggcatcgccc
     9781 gtgctctcgc gcagaagaat ttcgtgcacg ccgcgcgtaa ccgtctcagg catttcgagc
     9841 cagccctcat acgggtagtc ctcaaaacgc agccacgcgc cgagcgcgcc cacgagccgc
     9901 cagccacact cctctttttt gagaaccaaa gcgacgggat tctccaatct cacgaaagtg
     9961 agcacggcct cctcgccgct ctgctcgtca agattgacgt acttcaacac ggcgcgctca
    10021 gccctttcgg gatagtcacg atccctctca taatcgtaat aggtggtcgc cacgcccgga
    10081 cgcaaaaaca gacggcggaa gacctcacgc ctgaccgaca cgtcttcgct cttgagtgcg
    10141 gcaagtatct cttcgggtgt agagaagcta tcaactgcgc cgcaacgatc ttgacccgga
    10201 ggctcgacgc gcatcgcgtc ttgacgatgt gagcgcagga agcccatcac gaagttcacg
    10261 atcaggaaca gcacgaagcc gatgccgaag ccgattaaca atgccgacag gcgtgatttc
    10321 cagtgacgca tcagaatttt cctatttcga gttaagtcaa aagcagggtt aatttctccc
    10381 ctgcgctgcg ggacgcttgc cgggctgtaa cagctaccct tagcgacgcg cgatgagact
    10441 ctgatcgacg ctgtaggtga ccacagcccg gcgcgaattc tcttggcgta gcgagagcag
    10501 ttggcgtatg cgcgcctgtg ccgattgccg ctcgaccatg aggggaagca gtttcagcct
    10561 cgccgtctca aactcgaccg cgcggtcgat gtctccgacc tgttccgccg ccgctgaaag
    10621 caagcgcagc aattccctgc gcgactcaag ctcacgctcc acggcacgct ctctcagggt
    10681 tctgtagcgc gtgccatcct tcggcgcgct ctccggtgca tcaaactgcg tcctctgccc
    10741 ggcgtcgccc gtgctgttgc tggcatcggc ggcagcctct ccgctttgct ccgtgccctc
    10801 gctctctttc aaggtcgggt caagcctgga ggcgaggagg gctgcgcgcg gttgtccctt
    10861 catggcatag aagcggatga tccggcgcag cggactctct tccgcgaatc cgaaagcctc
    10921 gaccaccgtt tcgtccttgc ccgcaacgag ggtatggatg aagcccttga gttcgccccc
    10981 gtcctgctgt cctctttcgc gtagcgcgcc gaagaagttt agataaggat tcgggttctt
    11041 cgcttcgagc gcgttgaaga gtttggcgga ctcaggcgtg cgcccggcga ctgagagtga
    11101 gagcgcggca agcatctctt catcacccgc gttgagtgcg cgcacgcgct ccagcagcgc
    11161 atcccgcagt tcgtatctca cgccgatgat ctcaggcgcg aggtagactg cctgccagcg
    11221 agcgcggcgt gaggccgtcc ggtcgccgat gatctgcgcg agttgcgcga cggcctcttc
    11281 atatctctcc ttttcgacca gaagccgcgc caactcgatg cgattcgttt cctcttccgg
    11341 cgacgcctcg aataactgct ggcggtaatt gatggccgcg tcgaactgcg taaaggcggc
    11401 ggcggtttct gctgcgagtt gcagagtctc tatgcggtcg agcctgtttt ccgtctccgc
    11461 tccgtcctgt atggcgcggt cagccgcata aactttgatg tcaggccagg aggccagatc
    11521 agccgccgcc gtgcccctcg tctcttcgcc ggagagagca accatcgtct gcaacagtct
    11581 gagtccgcgc gccgcatcgc ctctgatgaa ggccaaccgc gcgaggccgg tgaaggcgtg
    11641 cgcttcgtaa cgctcaagcg caagctctct cgtataagcg gcttcgagca gttggtcggc
    11701 ctgcgcggcg cgcccctctt ttctgagcat cgagacagcc atattcaaac gctcgatgct
    11761 cggcgggttg atcagagcgc gaccggctgc gatctctatc ccggcaaagc ggcggagtcc
    11821 ggccagtgct ttctccgcct gcccgtccag cacgtcgaga tgcaggctca tcaaacgcaa
    11881 ccacgcaggg cgcgcatagc ggcggctcac ttccgcctcg accgcgctga ttatccggcg
    11941 tgcggcagcc gtctcatgtc gcgcgattaa atagtccaga tattctctct gccaacgtat
    12001 gcgctcgccc ggcggctcgg aaattttgta gccgagttcc tgatcgagcg cctcttcggc
    12061 ctcgtccgcg ctccaggtgt tgtcgagttg cgcggtgtaa tcgtaatcgc gctcgtaact
    12121 gctcagagca gggctgcgct taatcaacag ttcgtagaac ggcctgagtt gtccctgcga
    12181 gatgagagat tctttaatcg acatcttcgg cagcaggttg ttgtcgctca cggcctcgct
    12241 caatctgtag aagaattttg ctcgcgcggc ctccgcctgc ggctcgcgtg gttcacccgc
    12301 actcacttcg tcgctggtca cttcgtcgct gccttctctg aatgagacgg cgagcgcacg
    12361 aatcagttct ctgaggctct taagctgctt ttgccgattt tcgccgtagg tgtttgcata
    12421 gaagtttgcg ccctcgttgt cgctttcatg gtcattgaaa accgcgacct gtgcgacgac
    12481 aagcgggaat aatttctctc tggcctctgc ggccaaacca tgcgcgcgaa gggtgcgcag
    12541 gtacagttca cactccgcca gccccggttg ctcgcccttg atgatctcag accatagctc
    12601 tccggctcta gctttatcgc ccgcaagaaa atgggctgag ccgagcgcgg cgcgcgcgtt
    12661 gttgtctgac gggttcatct caagcgcgag gctcagatgc tcaagcgcgc gtttgtgctc
    12721 cttccgctcc agcagcaagc gaccgagttt tgtctgctcg tcggggtcgc ggggtctgtt
    12781 ttcaaccaac gccaccagca gagcatgcgc gcggttcgcc cctgcgccgt cgcgcgcgga
    12841 ctcgccgagc cattgaccgt aagactgcgc cagttgaaac caatcgtcgc cgatgagttg
    12901 cttcgacgca tccggtttcc gggtgatgag ttcgccgatt gatttgagtt gcaaggcggc
    12961 gttgaaatag ccttcgctct gcgagttgta atcgccgagc gcaaggctcg ccccggcatg
    13021 acgcgagagt ttccaggctt cggagaaggg cgcgcgttca atcgcttcgt gcgccagttc
    13081 gcgctctcca cgtgcgagca gaaaattgat gagttgcagg tggtagggtg aattttgttg
    13141 ggctaaggcg cgcagttctt gtcttcccgg ctctccgttt tcgtagaggg catcaagata
    13201 gcggctcgcc agttcgttcg tcgggttcac ctgatttgga agatggcgga tgagcctgtg
    13261 atagttctct cttaaagcct gcaactcttt ctcgctgtcg ccgaggaggc gcgcgttctc
    13321 tgcgacgaca cgcaggtatc tcgcatcatt gccgtcacga ccctctgcgc gctcggcttc
    13381 caacagctca atgacctgcc tgtaatcgcc gcgcgcggtg tagaaatcga ccagcctttg
    13441 tagccgctcg tgatagaagc tctggttgcc ggtgcggaag gccgcgtcct tctgcgcgtt
    13501 cagaatcttc tcttccagca cgccgaagcc ggagcgatgg cttaggttct gcaatagctc
    13561 aagcgtctcg tagcgatcat tgttgcgcag ggcatcttcg atgcgccgat tgaagtcggc
    13621 gaacagtgcg gccaactcat cgcccgtgcc cttctgtgaa gcgagcgcgc ccacggcctc
    13681 cgggagcgcg ccgtccagaa cctgtagaga gttgcgcgcc tcgccggagc gcgttgaatt
    13741 tttgcgttcg acctctcgaa tcaacttgtc acgaagctgc cagagccgcc ctgtgatctg
    13801 atccaatctc tcctcttcgc gcacggtctg gacgtagccc gtaatctctc cggctttcaa
    13861 ctggtgtgcg aacgggtctg cgaaaaacgt gtcgaaggcg agacgataag cggcgacgcc
    13921 tttggatttt tcgacggcgc gcagccgtgc ggcatccgcg aaacggtcgc cgacagtctc
    13981 tctctttggt gcgaggtcgg gcagtttccg gcgcgcttcc gctgcctccc cgtggcgtcc
    14041 ggctttttcc agcacttcga cgagacgcct gatgtattgc gggtcatcgt ttgtcagttc
    14101 cgccgcacgg ttgagggctt cgagcgcggc accggtattc ttcatgcgcg tgtagacgtg
    14161 cgcgagcgcg tcgtagagtt cgaccatctc aggctggttg gcgattgctt cccggtaatt
    14221 ctgggctgcg gcgggcagat cgttccgggc atcgtagagg cgtgccagca ccacattcca
    14281 acgatagttc ttgtaagcct cttgcgacgt gcgacggtag taggcgagga gcgtgtctgc
    14341 gccgccgtaa cgctgtgcat aattgatcgc cgcctccacg ttcccctcgt cttcgggttc
    14401 gcggttgatg atttcgatgt gctgctcgat ggcggcgtcg taatccttga gccgtgtgaa
    14461 ggcgtcgatc atctgcttgc gaagctctgc cgtttcggcg tgcagctctt tgcggtcgag
    14521 gtcttgttcc ctgatggcgg cgagcgtctc cgcgaacgct tttttgaggg cgtcagtctg
    14581 tgaagtgcgt atcaggacgc gcgccagttc gtgaaagact tccgtgttca gtttatctgc
    14641 cgcatgcagc cgcgcgagca cctcgcgggc ggcgggcaga cggttcatca gcataagccg
    14701 ggctgccagc ttgcgcccga agatgtaata aaatcttccc ttgccgcgct gcatcgcatt
    14761 cgccagcacg cgcagagact cttcgcgcaa gcccatgcgc cagtaaaaac tttccgcttc
    14821 gttaagcacg ccgtagttgg tcgggaattt tccgacgagt tcgtgcagga cggcttgcgc
    14881 ctcctgacga cgcccgtgtt gttcggcgtc tgcggcgagt cgcagccggt acgagatggc
    14941 gaagcgcggg cttgccgcga cggcgaccag acgcttgagc gcgagtcggc ctacgccctc
    15001 atcttcgtct ccgtcgagaa tgttgcgtgc gctatcgaga aactcgcgcg agtcgctccg
    15061 cgcgatctct tgtttgaggt gcgcggcggc cagacgcttc tcgcccgcat cgctcagcag
    15121 gtctgcataa tcgagtacga gcgacgagct atcgcggcgg cgcgctcgct ctcgctcaaa
    15181 cgcttgttgc aacttcgccg ggatgccctc gcgccggtag agcttgacca gacgacgctt
    15241 cgccctgtcg ctctccaacc cttcatcgta atcggagcta taaaagtcca gagctttgac
    15301 atactcgccg agcgcatccg tcgtcctgtg ttcggcttcg aggatcgcgc ccgcctgaaa
    15361 agcgtaaaga gccggattgt tcaactgcct tcgaagttgc tcgatggtgc gcagcgcgtc
    15421 cgcgtactga aagtagtccc agtagacggt cgccgtctca aggtaggtgc cgggcgcgcc
    15481 gcgccccgtc ccgatcagtt gttcccattc tccgcgtgcg cgttcgtagt tccccagttc
    15541 ggcctgaatc tctccggcgc gcgtgcgata cgcggctgac gccggagatg attcggccaa
    15601 ctcgtgcgcg gctgaggcgg cctcttccag aaatttcgga ttgcgttgac cgagagaacg
    15661 cgtgaaggcg atcagacgct ccgagaattc aggcgtgttc gggtagagac gattcaactc
    15721 cctgtaagcg tcaatggcct cttcgtagtt ggacagacgc acggctgcgt cggcgcggaa
    15781 aagcttataa ggcagtgtgt ccaacacgcg ccctccggct tgcgtctgag cgaggcagcg
    15841 cgcgcgagcc tgatcgagat gggcgcgcag cccgttgcgg ctcgccagat gggagaggaa
    15901 ttcatctctg atcgcgctcg aaacaaaata gtattcggcc atcaacctgc tccaatctcc
    15961 ccagcgtccg tgcgcgctgt agaacttcaa caagcccctg acgaagctct gattgtgcgg
    16021 gaagcgttcg tgcgcgagcg tgtacaggcc gagataaagc cgcgcctcga aagttgaagg
    16081 gttcgcgccc gtgccttctc cgatgaactt gccgagatac tcctgaatct cgcggtcgct
    16141 caactgttcg agcagttcgc gcgagaaggt ttcaaactcc tgcttgcgct cgcgcctgag
    16201 aaaccagcgc gcgaggcggt cgcgccagag ggtcgtcgga aatttttgca acgcctgctt
    16261 gtggactttc agttgctctt ccagaagatt ggtttggccg agccattgaa gcatctgctc
    16321 ataaagcccc tgctcctccg ggtacttctt caactcgttc gcgtagagag acaggatggc
    16381 ttcggttcgt ttctctctgg cgagcgaggc gatgtggcgt tcgagtatcg aagcataggt
    16441 cacatcttcg ccgccctgcg cctctacgga gtggccttga gatttgaagg atacggtcgt
    16501 caggtgatcg gtataagggc tggcggaata gttgtcgtag gcgggcgacg tgctgccctc
    16561 ttctgcgctc tccgtgggga tgtctatgcc gcgattcgac tgcggcggat aggcggccag
    16621 cgaaggttta gtctccgtcg gctccgagta gatgtcgaga gccgactgct gcccgccgcc
    16681 ttccgcgccg gtttgggatt ggcctgcggc gggcacgagc ggtcttcccg tctcgcgcct
    16741 tcctcccaga tagtccatga tgcgctggta agcggcgcgt tcttcttccg cccttccgag
    16801 cgcgacgtaa cagtctgcga gtttcaaagc gaccgccgcg tactgcggcg cgtccccgta
    16861 tctaccttca aactccgcga gcgtctcttc ggcgatcttc ggctctttgg ccgccgcgta
    16921 gagccgcacg atgtccagat acatctgcgc caactcggta gaggtcggat actcctgctt
    16981 gtaagccgtg aagatgcgga aggctgcggc gcggttgaaa tactgcaccg cccgcttgtc
    17041 ttgatctttg agttccgagg cggggtctgt atccgaaagg atgagcgaga ggacgccgcc
    17101 gagcatgccc ggatgcgggt ctgaggtagc gatgtcctga taaaacttca gatcgccgcg
    17161 cgtgagcgcg agcctctgcc cgcgagcgtc ggccagcagc tcgaacaact ggtagagcac
    17221 ctttgcgcgc agagggctgc cggggttcag ttcgttgcgc acgtagagcg tgtagaggaa
    17281 acgcgaggcc gtgtcgccgt cgccgtccgc gatcaagatg cgcgcgagcg tcgccagttc
    17341 ctcaggcttc caactgatcc ggcgggctgc cctcgcctgc tcaagctgcg ccgtgatgcg
    17401 cggcgatttt tcgcccgtgt agttacggta atgaatgaga cggacggcca cgtcgaagtc
    17461 cgaggggttg cgtgagaagg ctccggtgag ttcgtggccg taggcgcgaa agcgatcccg
    17521 gcggctgagg aaagagtaga accggctcga catcccatca ggccagaagg gatcgaaggc
    17581 ttccaggtag acgcgctcgg cctccgcatc cctgttcatc gagacgagaa tcgaaacctc
    17641 tttttcgagc aggtcgttcc tgcgctccgg gaaacgctcc ttgtactggt gcaggacttt
    17701 gagggcttcc gcgtagttct cttcctcaga cagcttgtcc agaagttgtt cgatgatgcc
    17761 gtagacgccc tcatcctgcg cgatatactt ctgataaaaa cctggcttga gataattctc
    17821 cagacgatga acgcgcgcca gttcgacgag ccgcccgaag atttgcgcgc gccgttcggc
    17881 aggcgcgatg gcgagcatgc gctctatggt cgcagcctct ttctcaaact gcgctctccg
    17941 gttgtggaaa gcggcgagaa tttccagagc ttccgggctg cccgtttgaa gttggacgaa
    18001 cgcttccagt tcgcgctcgg cctcttcaaa gcgtcccaac ttttcatcca actgcgcgag
    18061 cctgagagtg atctgcggct cgtcaggata tgttttcagg acatctgcga gacgattgcg
    18121 cgcttctgcc gtcgggtagg gcacgagcgc ctgcgcgccg aaaaattccg tgcgcgcaaa
    18181 cagggcagcc tcgatggcct ccgccttctc atccttcacg cgccccggcg cagccgctcc
    18241 ccgcttatct tcggcggtga cgagtcgctg tgcgacgacg gcgcacgtaa gcgagatgca
    18301 gctgatcgcc gtgagcgtca atagtcgtcg ccttgtcagt ttcaacttac tcatacgtat
    18361 atgtagccgc tcaaatgcgc ggacgttttt tctcatcatc gctcgatcac ttcgacccga
    18421 acttccgtcg tagattgatt gtgcgcgaag tcttcagccg tgacggtcag cgtgtactgc
    18481 cccgccgcca gacccacggg cacatgcaga cgccccgtgt tcgtcttctc ctgcgccgac
    18541 cagaagagtt gcgcgggttg cgcgccgtaa aattttgcca ccagtcgtgc cgtatcgctg
    18601 tctgcgtcca cgcgcaattg aagctccgaa ccggcgcgca cggtcgctgt cgccagacgc
    18661 actttcaaac gcggcgcgtg gccgtcaaca acaaaactct tctcttcctc gtagccgttg
    18721 ccgttcctgt cggtcagcaa cagacggcac ttgtaagttc cgtcgggcat ccatgcgggc
    18781 gcgaagaaac gtccctccca gacgccttcc gtttgcagaa atttgagcgg cagagtttca
    18841 ccgtatggca gcacggcgaa cacggacgtg atggacggat cggtcttgac gcgaatgaca
    18901 ggatcgcccg gctgtatgag tcgcgggcgc aagagggcgc gcggcgcggc gagaaaggcc
    18961 gtgtacggcg tgacaaacct gtatttttga gagagccgaa tgatctcggc gatgtagtct
    19021 tcgcgctcgc cgttcaggtt catctcttcg aggagctttc cgacgcgcgc gcgtccccac
    19081 agacgcggga ggtgctggtg cgtgtcctcg aattccggca gcgacacctc gcgcgaaaga
    19141 ttgatcgctc ctgtgccgta ctgtcccgat agactcaggg cggtttgcgc ctgcgccttt
    19201 ttgtaacgac cgacgaagga gaagcttgag ccgtcgaagc tgttctcgcc cgtcgcatag
    19261 acctgataaa agttggaagg gtctgcgggc gtcaggcgca ggttctcgat gccgggcgtg
    19321 ctgaccttgg cgaagagaat cctgagcagg gcggagatgt cttcggtttc acgcgcgtgc
    19381 gcaaaatagc cgtgagtctt cttcgccaac tctgcaagca gcgcatcgtt agcgtcgctg
    19441 ccgagggcta aagcgtagag acgcgtgtgc gtcgctttat cccgtcgttt ggcttcgtcg
    19501 tcgaagaggc gcgcgaccgt tttcaggctg gtcgtgccga gcgtcgggtt ggcgtcggag
    19561 atgagaacca gattgcgctc tccgtctgga aaggattgcg tcgcggccaa cgctttctcg
    19621 aagactttcc tgagatcagt cccgccgccg agcgaggagc ctttgatgaa ttgaagcgcg
    19681 tgctcgacat tctccggcgt cgcctcgacc ggcgcgggcg agaagagatt tgtctcctcg
    19741 ttaaagagaa tgaggttgaa acggtctgcg ggcgagaggc cgtgcagaaa atagtccacc
    19801 gcctcgacgg cgcgcgtgag cttctcgccg tacatggaga gcgacgtgtc gagaagcagc
    19861 atgatgttgc gcggcgcggc gtggctttca cccgtcgcac ggcctgccgc cgcgtcgccc
    19921 tgtgtgctgc gcccctcgtt gaagatggct cgcgcttcga agtagccgtc agggttgcgc
    19981 gcggcgagcg aggggtcgcg caggtcataa gcggagattc gttcgggggc gcgataagcg
    20041 atgaaggaga gcgcgctctg cggcgcgtcg atcctgtaat caaaagagaa gtcctctgtg
    20101 agagcgatct cccgcgcccg aaactcggcc tcaaactccc ccggctgcga tttcaagacg
    20161 tggagcgggt agcccttctg gccggggtct gaaaccgtga tcgggtaatc gccgagcaca
    20221 cgaatgtgca ggttgaactc tccgacgcgt tgcgcgtcgc cgaaggaagg cttcaaggga
    20281 aaagtgaaat gcgagttgag actctcgacg gcaagcatct ccgtatattc catctcgacg
    20341 cgcttcgagc cgtaagggtt gatggggaag accttcgcgg agaaagccgt ctggccttcg
    20401 tgttcatcgt cttgttggag cagacccgga tcaaccgccg cctgcttgat ctcgccatag
    20461 acggcgtttg cgcgccgcct ctccatcatc acgccgggga tgcgcgtgtc gccgtcccag
    20521 acggcgaagt ctgcgaccga cgcagcgggc ggcagtgcga acaggtattt gccttcgagg
    20581 gtctgcgccg tgtggttgtc gtagatttgc aggacgcgca cggtcgcgtg ctggttatct
    20641 atgagcacgt ccacgttcat cacctgcaaa gagaggatgc tcgcatccgg tttctctttc
    20701 gtggacggga tgagcgcgcc ggattgagcg cgcccaggac ggacgggaag acccgcgacg
    20761 agaatgagcg cgagcagcag caacgccggg atgatcgccc gctctctgcg tccggttctc
    20821 ttttgaactg ttgtctctct ttgcatgatt cagttgaaag cgcggtttgg tgccgcaggc
    20881 agtagtgacc gggcgtggcc gctcagattc cggtcaggta acttttctcc actcgcgcga
    20941 tgccgttcgt cgttccgaaa tagacatact tggcgtcgcc ggtgatgctc aaaacggttc
    21001 tcgcaggcag ttcgtctttc aggtgcgtcc atttttgtgt gtgaaggttg aagacccacg
    21061 cgccgccaag cgtgcccacg aaaagcatct ggccgtcgct ccacatcgcg ttgggattaa
    21121 cgacgggcgt gcctatctcc tgcgtgaagc cgtgcagttc gtttgacggc gcaagctcga
    21181 agacgccgcc gccataagtg cctatgaaga ggcgcgcgcc cgccgcgcac agacccgtca
    21241 cccagttgtg tgtgagtttc gagttggagt ctttgaagac gcgcacgaca cgccctgact
    21301 ccacctgcgc cagcccgccg agcgtcccga caaagagcga gcgattgaag aagagcgtcg
    21361 tatatatctg atcgctcggt agtccctgca cggtcgtgag ggcgcgccac ctgccctgtt
    21421 gagccacccc caacccgcgc cccgtcgcca gcacgagttc acttccgcgc gcggcttttg
    21481 ccggggcaga gtctccgcct ccggcgcgcg acaggtgcat gatcgagttg ctgggcagcc
    21541 cgtcggcctt tgtcgtgctc gtgctgcgga actgagaggc gaagcgcacg acgccctgcg
    21601 aggtcgctgc cagcacggcg gattccccgc ccccttccgg cagcaggaag ttgatttccc
    21661 ggaccgcctc cgattcgaga tgtgcgatct ttttgccctc gccggagagc acgtctatgc
    21721 cgcgtctgaa attgcccgcc cagaatttcc catcggcgtc gaacgcgagc gcggagatca
    21781 cgttgctcgc cagcgtggga gcgctctcgc tcgcgtggcc gaaacgcgcc agtgagagcg
    21841 gcgcgggcgt ttcggaagat gcatcttccg gcgtctgcca gatacctctg ctgccgagta
    21901 gccagagcct ctcttcaagc aacatcaagc ggctggccga aagatcgaaa ggcttcggcc
    21961 agacgatttt cctcaacggc ggggaggggc gcgcggctgc ctcctgcggg ttgagcgcga
    22021 gaagttctcc gttatcttta acgatgagca gaaggccgcc gtagctcacc agcccggaga
    22081 gcgtgggcag cgtggcgagt gtgtgaaagc gtctctgctc agacgggtgt gtttgcgaca
    22141 ggctggcaga tgtggccgta gcaagtccga aatcagaggc gacgaaaaga tttccgccgc
    22201 tcgccgcaac tcccaccacg cggtttgaag cgaggccgtc ggcagaacgg aaatgcagcc
    22261 agcgtccggc ttcgaggagc cagaggccat cgttaaatgt cccgacgtac aggcgcgagc
    22321 cgtctttgaa gagacagttg acggcgagca ggcgcgtgcg ttctgcgccc gccttcaact
    22381 ccgtaaagcg tcgcccgtcg aattcgatca gcccgccacc gaaggttccg cagagaagac
    22441 ggccaccgtc cgcgagcatg gaggtgatgg cctgcggctc gcggtccagc cagcgatagc
    22501 tctcgaagcg ttcgccgtcg aaagagagca agccctgcga ggctgtcccg atgaacagct
    22561 tcgagtcgaa gacggcgaag gagagcaggt cgctctccgt cagcccgtcg agcaccgtgt
    22621 agtggcggcg aaactgtccg tccgtggaga actgaaccat gccgccatcg gtcgcggcga
    22681 aatacgaatc ctgaaagcgg atgacggcgc gcgtgctctc cgtgttctgc cagaacttga
    22741 tctcggttcg tgccagcgga gcgcgtatgg atttttcaaa aggaataaat tcctgaccgg
    22801 cgacccgcgc acgttcctgc gccagcctcc gctgcgcttg attcctaatc cagacgacgc
    22861 ctgacagaaa tgagaacagg cacaacccgg cgaggcaggc cagcaggagt ttacggcgag
    22921 caggagacac agcccccgtt tgccatgtga tctttcgcat cgtgctgctt acggatgttg
    22981 acttgtctgg catcggttcc tgtcaaaagc gatcttcggt ttggctcgac tcgacgtgat
    23041 tgtgcagtgc gtgtgattta agagatacgt gacgacgaac agcgttcgac gcagttatcg
    23101 ccatcggggt aggacgtgtt acacgcgccg ggcactgtgg ctaaccacag tgtttggaga
    23161 cagcgcgcgc caacccggtg tcgaattgca cgagccaaag accgcttgaa gcaacttcaa
    23221 acggagcgat gaacttgtag attgatcggc tttgcaggcc agccgtcagc cggacagcgg
    23281 cgcgttggct tgtgaggctc tcggtttgcg aagcgtgaag atcgtgccgc cggaggggct
    23341 tttgtcccat gagacatcgg cttcgatgag gctggcgcgg gcgcgtatgt tggcgaggcc
    23401 gcgccccttt cttccctttc tggtgtgcgc gtcgaagccg cgcccgtcgt cttcaatcgt
    23461 catgacgaaa ctctcatccg cagagtttgc gacggcgagg cgcacgcgcg tggccgacgc
    23521 gtggcgacag atgttgttga gaacttcctg cgcgatgcga tagacctgca tctgcacggc
    23581 gggcgcgagg tctagccgtt cttccagttc ttcgtcgcag acgaactcat actcgaactt
    23641 gcagccgggc gcggcgtgcg ccgaggccgc attggtcaaa gcccactcga tggccgccgt
    23701 caagcccaca ttctccagca cggaggggct taaatcctcg cagatgcggc gaatctcgtt
    23761 cgagacgcct tcgatctccg agcgaaagac ggacgcatcg aggctcgact cgtggccgtt
    23821 gccctgcggc gcgctcgcag cgggcagccg gtctatcagc atcaggagat gtcggaggtc
    23881 tgcgagcgtc tggtcgtgca ggtcgcgcgc gatgcggcga cgttcgcgct ccgcttcgtt
    23941 cgccagatcg agacgcgcgc tcgccagttc acgattggcg cgcgagaggg ctgtgctcgc
    24001 gcgtgcgatg cgttggtgct cgacgatggc ccacacgagc gcgacgagca cgagtgcgag
    24061 cagtacggag agcgcaacag tcgtccacgg gaaaggagct ttggcgacgc tgaaattaaa
    24121 gttgagcggc gcggaagcca gcaggtcttt gctgaaagcg cgcacctcta cgcgatactg
    24181 gccggggcgc aggttctcta tcaggaattg cgaatcgggc gaaagtttct tcttgataat
    24241 gcgcccggcg gcgtcgcgca ggaggaaggc atactggaac tgttcgggaa aggtgcgact
    24301 gctcagggcg gccacttcga gcgcgagact gttctgcggg taatccagat tgatgcccgc
    24361 gcgcatctct tcgggctggt gcaggcggcg gctgagaatg cgcgtcggga gcagcaaggg
    24421 cagcgtgttg ccggggctgt aacgaacgac gccgcgattc gttccgatca ggagagactc
    24481 cgcgttgccg tcgccgcttc cgtcacgcag agggaggatg gcgaaggctt tttgtgaagg
    24541 gagtccctgc tcgacatcca gacgcgagac gagtgtgccg aactctgtgt ccgcgacgat
    24601 gtgcagcagg ccgcccccgg cggtcgcgca ccagacgcct gtgagagccg acgttccgcc
    24661 cagattttcc gcgacgacgc tacgcacatc cgtatccggc gtaacgctcc gcagttcgcc
    24721 cgcgcgataa ctgtagaggc cgcgcgcggt ggcaaaccag aggccgccgt cgcgtgtgcc
    24781 gtcaatagcc cagacgggac tgccgcgcag agtttcgagc gcggcatcct gcgtcgcctg
    24841 cctgccgtcg aagataaaga cgccgttttc ggcagtgccg atccagagcc tatcgtcgcc
    24901 gtccgtgtgc aggctggtca cgtttttcag gcgcgcatcc ggcgatgcga caggccacac
    24961 gagcaaacgt ttttctccct ccacacgctc gaccccgcgc ccgtagaccg ccacatgtgt
    25021 cttgccctga aagatgcgaa tcgcgcgcac gctctcgacc gcctgctgcg gcgacgcttg
    25081 agcgtccgtc tcttccgtct cttccgtctc tgcggggatg gcgtgttgat aactttcgtc
    25141 atcgcgccct ttcaggttca cgttgacgta gaggccgctc gaagagccga cgagcagttt
    25201 ccctgaagcg tcttcggcga tggcatagat cgtccggttc actaaaccct gcaccggaag
    25261 ccagcgtgct gccgctgtgt cgtagatgaa gagtccgcga ttcgtgccgc acagcagttg
    25321 gccgtctctg gtttgataaa gcgtgcgtac gaagttgctc tgcttgtctg cggagacatc
    25381 ttcgatgcgc gggctgcgcg gatcgtagcg gcagacgccg cgatccgtgc cgaaccagac
    25441 gaccccctcg cggtcaacga agatgacata aatttcatcg gagcgaaggc cgcccgccgt
    25501 gccttcaaac gtgaagtgat cgagttgccg cgtgccgcga taacggaacg cgccgcgtcc
    25561 gcccgtgccc acccagaggt cgccgcgatc atcgaggccg agagccatca ccgcgcctgt
    25621 gttcagatca atcttcgagg ggcgtgcggg atcatccgcc atgtacaggc cgctctcccc
    25681 gcgcttcgct ctcgcgccga tccacaagtg cccgtcggca gcggtcgcca gcgcattgat
    25741 gaagaaggcg cgcgggcgac tctgaatctc tttcgcttcc tcgccttcca acgccaacag
    25801 gccgcgactc tgtgtgcctg ccaggagtct cccgtcgtac atggcgaggc tcgttattcc
    25861 gagtgcgccg ggctggtctg catctgcgct cagcaacgtc tggcgcggga gttgtttgac
    25921 ggagagcgtg gtgtcggggt tgcggcggca ctcgaagatt agaccctgcg ctgtagaaag
    25981 gacgacgcgc cctgcctccg gcgcgatgat ggcattcacg gccttgcccg caatctctgc
    26041 gacgcgtcta aagtcgtcat tcaggaattg cgctgcgccg ctttccgtgc cgacccagag
    26101 aatcccgtcg ccgtccactc taagcgcgag cacgcgccct gcgggtaagc cttcggagac
    26161 tacggcctgc acgcgtcgcc cgtcatagcg cgccagccct tgatccgtgc cgaaccacat
    26221 cacgcccgcg cgatcctgcg cgatggcgcg gatgcgatcc gacggcaagc cgtgaaacga
    26281 actgatcgct ccccactggt gcaggttctt cacacgcgcg gaagaagaat cttcgttcag
    26341 agtctctgcg cgcgaagcgt cagtctcgct ctgcgcgaat gagacgaaac cgcaggccag
    26401 tccgagagag agtcccgata aaaggcagat gaccagcagg ggatcgcgca aagggagaag
    26461 cgcgcgaccc ggaaagcctt tcgcctcccg gcttctgccg gacgctctga cacgcctgtc
    26521 gctttcagcc ttccgccgtc tcactgtttg cccttgcctg tgccacgttg ttcttccaga
    26581 taacgttggc ggtcgcgctc gatccagttt gaaagctcgc gctcggcctt ctccagttcg
    26641 tcgctgttga tcaggccgcg acgcagtgct tcaaagaagg cgcgcgtgcg cacgttgtag
    26701 gcgagcttgt cgtaagcctc ttgagccgtg ccctcggaga catctacgaa aagtttttca
    26761 tagagggtgg cgaggcggct ctcgacgccg cgcagggaga ggctgcactt gcgcgcgacc
    26821 gcccagttcg aaaggccgag cgcgagatag tagagagctt cggcctcgaa ctcggtcagg
    26881 agcgggcgtt tgaaggagtc tttgaaggcc gggtcaatca tgtccgcgcc gtcttcgagc
    26941 acggcggcga cgaagcgcag gaagcgcgac tcaggattgt tcttatgtat gaacccgtaa
    27001 gcgggcgggg catccgcaga gcgtatgatc ttgcgtatct cgttgatgta aacctcgtgc
    27061 ggaaactgcg tccagaagat gatgcgcgct tccggaaact cgcgccagat cgagcgcgcc
    27121 gccttgacgc cggaaagctg cggcatctgt atgtcgagca cgcacaactc cggcctgtgc
    27181 ttgagagcca gttccaaagc cgcctcccca tccgccgcct cgatgacggg cgcgtgcgcg
    27241 ggaaattccc gcgccagcaa atcgctcaga tagcggcgtt gcgccacgtt gtcttcggca
    27301 atcaggatgg acatgtgatc aggcttcggg cgtttagaat aaactcctca tctcacgggt
    27361 gacgaggata cgagttgcag cataataaag catagagccg tcgcgttgag caatgtggat
    27421 ttccacagta gggcgacgcg gccagcctgc ctcaaggcgg gtagctcgcg tgtttctgcg
    27481 cacacgtccc tccgtcataa tcgcccggat gttaaccggg caagccataa ttccgcgccg
    27541 cttcttctta cgattgcgca cacgtcttct cctcgtcccg ttggtgtgcg cctgtgccgc
    27601 cgccctgacc gcttccttcc cgttgtggga gttgaaagat aagaggcatg cggcggagac
    27661 gtatgcggcg cgcgatgaag atgagattga cgcggcgttg gagcgcgcgg cgcgcacggc
    27721 gataggctct caggagggga cggtcgttgt gatggacgcg cagagcggtc ggctgcgctc
    27781 tgtcatcaac ccgcaggtgg ctttcgcgca agcctacgcg cccggttcgg cgatcaagcc
    27841 cttcaccgcg ctcgcggctc tgcgtgcgca tctggttgac ggccagacgc gccgcttctg
    27901 tcgcacgcgc tatgcttaca gggaactcac gattgattgc tcgcaccagc agtcgaacgt
    27961 ctcttttaat ctcacgcagg cgctcgccta ttcctgcaac cacttcttcg gcaaaatcag
    28021 cgaaggattg gagacaggca gcttcgacga actgctcggc tcgttcggct tcggcgcgcc
    28081 gaccggcctc aaggcgatgc aggcggagtc ggcaggcaaa ctgccgcgcg gcgagtggcg
    28141 tgccggttac gcgctcggcg aaagcgatca actgctggtc acgcccattc agttgctcac
    28201 ggcctacgcc gcgctcctga atggcggacg cctcttcgtg ccggggcagg ccgaacgggt
    28261 taactttcag acagtggaac gggcgcgcct gacgattgcg cccgaacacc gttcgctgat
    28321 tgtcgaagga atgaagggcg cgctccgata cggcacggcg gcggatgccg gactcgacac
    28381 cttgccgctc gacctgttcg gcaagacggg cacttccacg aggtcgaatg attaccgctc
    28441 gcaaggctgg ttcgtgggct ttgccgccga cctcgattcg gatgcgagcg caaggccgga
    28501 gagcgtcagt ctcgccgtcc tcgtttttct caagcgggcg cgcggggctg acggcgcacg
    28561 cgtggcacga tctatttttg cggagtatgc aaagcgtcgc gttgaagttg aaagagggag
    28621 aagcgagaag caggcggtcg cctcaaaccc ggcacagctt tccgcacaga gagttcgtgt
    28681 tcatctggta agccagaaca tcacgcgcga cctctctctg gaagagtatg tgatgggcgt
    28741 gcttgcagcc gagggcagca tcgagagtga acttgaggcg ctcaagtcgc tggccgtcgt
    28801 cagccgcgcg tacgcgctcc acaatttgca gcgtcacgcg agcgaaagct tcgacctttg
    28861 caacacgacg cattgccagc gttatctctt cacgctcatg gacgagagaa aacatgcgcg
    28921 cttcaaaaat ctcttaaagc gcgccgtcag cgaaaccgtg ggcgagactc tgcgcgacga
    28981 acgggggcag atcgcggacg cctattttca cgcggcctgc ggcggcatga cctccgacat
    29041 acagacattg tggggcgtcg tcggcgcacc gggctatttg agcggcgtgc gcgacgagta
    29101 ttgtgtggcg gcgcggcaca gttgggttaa cgtgatcccg gcggcgaaac tggcaaaggc
    29161 tctgcaaggc gactcgcgca gcgacgttgg agcgagactg gaaaatatcg tcgtcgctaa
    29221 ccgggacagg acggggcgcg tcgagttcat cgcgctcgaa ggagagaaca gacgtgtact
    29281 caggggttgg gatttcaagt tgatcgtagg ccgcgcgctc ggatggaata tgctgaaaag
    29341 ttcccgcttc gacgtgacac gcgccggaca gaattttgtc tttcgcggtg acggcttcgg
    29401 ccacggcctc ggcctatgtc aggcgggagc gcacgctctg gcacgcaggc gcggcgcatc
    29461 ctatcgtcaa atccttagac actattttcc cggcacaagc atcggagggc gagaagcacc
    29521 cggcctgcgt cgtcaggaga cttgatgaga ggttcgctat cgcgcgctgt aaccgagagg
    29581 cacgcgatag gggctttggg tgcatgctgt tgttagtctg cggcagacca cccggcgggt
    29641 ttatccgggc gtcaggcgac tacttctctg cacatgattc cagacggcct cacttgatta
    29701 agttggcgac ccacgtatag acgaagaagc cgagcaggaa aaatatgccg atgagcatcg
    29761 ccgtctcgaa gttgtagagc gagacgagga aggcgaggag cgcgagcgcg ccgagcacga
    29821 cggcataagc gggcaagacc tttttcgccc tcggagtctc ggcgttgaac gcacccgcaa
    29881 tcggcatcgt cataatccca gacccggcgg cgagcaggta gaaggaaggt atgcccgtca
    29941 gcagtgcggc ggcgaggctg actacggcca gcaggagcgc gccgccgacc cagttcgacg
    30001 ccgtgatctg ttttttcgag agcacgacgc gccccatcgg gtcgagccgc agcaggaggt
    30061 cgaagagcgg gcgggcaatc cacgtcatca ggacgaacgc gagatagagg ccgtagagcg
    30121 gccacaggat cggccccagt tccgggttcg acttcaacgt cgtgcggata aggcgcgaga
    30181 gaaagtagaa gccgatgatg aatccccaca tcgcgcggct cgacaggcgc gacgaccaga
    30241 ggaagtaacg cagcatgacg cggtagaccg gattgcgcgc cttcagaact tcgaccacgc
    30301 cttcacgcgc ccactcaagc tcggcgtcga ttcgcagcgc ctcgcg
//
LOCUS       DASWNS010000325        11324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_428515, whole genome shotgun sequence.
ACCESSION   DASWNS010000325 DASWNS010000000
VERSION     DASWNS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11324
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428515"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="VGB76_11395"
     CDS             <1..1272
                     /locus_tag="VGB76_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099544.1"
                     /translation="LFVGLEDKLDAWAGLPSVSEGTVTGVSSASTPLPGQGSPRAHWG
                     VHHIKSNMTAASTGFLPNVPGGSELEQLLGELWTLIQNEAATFETAIENLRTQVVDQI
                     TTLPAGEIVKRVIAIFLDVLLDTIKNIALAVIDILQLFLQGALDMLDKKLDIPVISWM
                     YKLISGDDLSILDLACLIVAIPATVIYKLAKSEVPFPDDAATDALIHAPDFATIRKLL
                     GGQTATGERTPAEAALGASSAAGVVPVVFDIAAYFATLGLIPLTGWKAANPNSKPAYI
                     LYAILYLPYIAPGIQLYTPQTWDVKLNERITGLSLLKSLADVGLYRYKKGVPDQSKGL
                     EDWYKVSPYVEAGINALWEIPAIAGIVRSQDTEAVIGFLGNTAFNLSGVLSPKADNDY
                     VFAAILICMGIYGELELVTGLTGSTEQLALT"
     gene            complement(1412..2077)
                     /locus_tag="VGB76_11400"
     CDS             complement(1412..2077)
                     /locus_tag="VGB76_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099545.1"
                     /translation="MKSIACALTLALALLSSASLRPATAQTGVTSATGSYKFVSADGL
                     AKYVEFSATADAKGTTTGRLSFSGQLVMSEQDAAAAGDPRLAGSTTDFYMKAEFDCLT
                     VYKNRAVMGGTVREAAPKSYAGRRVLLVVEDSGVDIKTGDRLTWLLYDGTTGSWVPKD
                     AERADDNGATLTWIAKDAERTDDVGVRQPKNSSVIGCRDYPLSAHIFGNVNYQSGDIR
                     VQP"
     gene            complement(2305..3003)
                     /locus_tag="VGB76_11405"
     CDS             complement(2305..3003)
                     /locus_tag="VGB76_11405"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY0099546.1"
                     /translation="MNVPIQPIYLFADSQLLFWKNQGVLFLNSLRQLLENDAPKAAYV
                     GASNGDAPEFYLIFEAAMEGIGIHNCRMVRSSFPAEDEAYLNEADIILLAGGDVAAGW
                     KVFEESGIKELLIRRYYEGATLIGTSAGAVQLGLCGLIDGGQPLEELLHTFKLIPLVI
                     DAHDEKQEWVRLKRTVRLLDGVEKGIGIPTGGGLIYYPNQSIEAIRHPLYEFSIRDGE
                     IREALLMPGNERAE"
     gene            3302..4513
                     /locus_tag="VGB76_11410"
     CDS             3302..4513
                     /locus_tag="VGB76_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099547.1"
                     /translation="MMFRINRLPLCALAVLLLAFQSTAQQEITHHQLAATYVSGHEYA
                     SGALTLQTDGRYSMEGGSHDGTEFSESGTYVLAGGVVRFTIERSVKRRRGVEGEINLL
                     EPQESREGKEGSGSNSTAGIAREFSLIPFRWSERIYLIKEDDLINFVNAVNLGLEPRT
                     ELRSENYYGAFYLRRGDELKRASGHPSLPAKWLSFLLSKSVTATVTAIEESQKGSFSA
                     SSTARIDKGSRDGLKVGMRLLINEEEPSTSLGAEIVSVEERTARIRIAQVNAELKIGD
                     VVRTRDEPKLDEYGALSWEEEKKRLARFAARLLQRPDAQGYVIAYDGRRARAGEALAR
                     AERARAYLEKELKMESGRIVAVDGGYREDQAVELYAVRSGTKPPEPAPTINPGEVRTR
                     RGNVKPGSSRR"
     gene            complement(4646..5125)
                     /locus_tag="VGB76_11415"
     CDS             complement(4646..5125)
                     /locus_tag="VGB76_11415"
                     /inference="COORDINATES: protein motif:HMM:NF036804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEY0099548.1"
                     /translation="MDIDLQECPCGGGEFAQCCQPIIDGKVQASTPEQLMRARYSAYA
                     TGAIDFLGKSNHSKTRRQFDPESAGRWSRESRWLGLTILGSDSSTPDRAHVNFEARYE
                     DKDGTAILHRERSLFEREEGDWRFVSGGAIPAVSQKVGRNEPCPCGSEKKFKKCCGA"
     gene            complement(5344..6492)
                     /locus_tag="VGB76_11420"
     CDS             complement(5344..6492)
                     /locus_tag="VGB76_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099549.1"
                     /translation="MNIIERPADNYSRRLKQIADQLLSQREDADRLRVAATELYSLCS
                     ASTGIDEDSSYADNSHETVLPNGKALSPRDAASCVLDYSRTSKLLRGIHAAILEAQRR
                     FPNTTIEILYAGCGPFAPLAIPLTSRFNPAEIRFTLLDIHRRSLDAAQSVFQAFGATP
                     FVRDYIRCDAASYKHDAPHVIHIVVVEAMQAALEKEPQVAITINLAPQLCAGGIFIPE
                     RIVVSCHLCDPTNEFSSLHVEAKAGNSLSPGSTRNKVRVDLGSVLELTAGSSLNLSAP
                     LDGSEHSGASLSPKVVIDVPKDLDGEFNLMLSTTINVYDSIVLSEYESGLTCPRILYE
                     LGKIQGGMRIEFAYHPGDKPGFKYRSLSSCGGQSVSPDKRSIIGYPLS"
     gene            complement(6699..8678)
                     /locus_tag="VGB76_11425"
     CDS             complement(6699..8678)
                     /locus_tag="VGB76_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099550.1"
                     /translation="MCSCLAAGTLAQTASPSPSPEPVKTPETKPAEQAGGNPFAPESA
                     PPLPAGMKGSDANDPRAKLSPGIYDAGEAAMGMRHLMLLKKPDAFQLGSDDPDNPKVQ
                     KTLGVLGMGDASKIPKPLQLVLAQLAFANSDLAFQGNHLFMGNFYGVSIYDIADPAKA
                     RLLTSMVCPGGQGDVSVHKNLLFMSVEMPNGRLDCSPQGFPPEPPPAAGQENVPTLPA
                     AQKERFRGVRIFDISDIRNPKQVAAVQTCRGSHTHTLVVDPKDKNNVYIYVSGTSFVR
                     QSEELAGCSGEPPDKDPNTALFRIDVIKVPLAAPQNAQVVSSPRVFMDPRTGALNGLN
                     NGGTHGKNGLEKPSPTDQCHDITVYSSLGLAAGACSGNGILLDIKDPVNPKRIDAVND
                     PNYAYWHSASFSNDGKKVVFTDEWGGGLGARCRANDPNVWGANAIFRLTGNKLNFASY
                     YKLPAAQGDSENCVAHNGSLLPVPGRDIKVQAWYQGGISLMDFTDPGHPIEIAYFDRG
                     PVDPKMLVLGGAWSAYWYNGHIYASEIARGLDIFELTPTQHLTQNEIDAAKTVRVAEL
                     NVQNQEKIEWPRQLIVAKAYVDQLERSQALPSDRIAAVRQAIQRAESSQLNPGELGKL
                     KSLAPSLEKSAGLTKNAADSARLHALAEILRRPAR"
     gene            complement(8726..9490)
                     /locus_tag="VGB76_11430"
     CDS             complement(8726..9490)
                     /locus_tag="VGB76_11430"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEY0099551.1"
                     /translation="MLDQSTLMQSFTFSAGLRPRPIASVAAPQSLLMAACALMCFLSL
                     PVYAQQADSKPPVVVQPGAPGQPTQTLPPSTRATLPPRSPKDVEFMQGMIMHHAQAVE
                     MTALIESHTENRELRLLGARISQSQSDEMKFMRRWLEARGEPVSPPKHEMHGMDMPGH
                     DMSSHHTLMPGMLSPKQMDALRKAKGAEFDRLFLNGMIQHHNGALIMVKDLFDTAGAG
                     QDAELFNFATDVDSGQRAEIRIMQTMLGEKPVEEKR"
     gene            9799..>11324
                     /locus_tag="VGB76_11435"
     CDS             9799..>11324
                     /locus_tag="VGB76_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0099552.1"
                     /translation="MKLRDTNAAALLIAPLAATLMMCAATVASAEDWPQFRGPNGSGV
                     TASPGLPEVFGPEKNVVWKTPPPPGHSSPVLTRDRVFITAYAKEKAIGTQQTVAGTKG
                     NAAEKNHKLLVICLDRQTGKLLWQREVPRSREGRLQNVNDPASPSPVTDGTNVYVFFQ
                     EFGLVSYDRAGKERWRLPLGPFNMFYGFGASPILVDDKVILPVDQDSPSSYLIAVDKN
                     NGRVRWKVERPAVISGYSTPIIYQPKQGAKQIVIPESFQLSAYSVADGNRVWWVRGLA
                     CEMKSIASSDGEYVYINGWGFPQNQPGNQISTIPFEESWARYDKNNDKLIAKSEVTGA
                     EKMDKMLAAAFEAFDMDRDEKLNAKDWEVFRGMMASENGLLAIKLGGQGDQTATAIRW
                     RYQKPVPQVPSTLLYKGVLYMVNDSGIMLSFDPATGNVIKQGRLHGAIDKYFASPVAA
                     DGKVFLVGQGGQVSVLKAAGDWEVLAVNELDDEVFATPAIADGRVYIRTRSALYSFGK
                     "
BASE COUNT         2410 a         3242 c         3291 g         2381 t
ORIGIN      
        1 ctcttcgtcg gcctcgaaga caagctcgac gcgtgggctg gcctgccgtc cgtctccgag
       61 ggcacagtca caggagtctc ttccgcttcg acgccgctgc cgggacaagg cagcccgcgc
      121 gcacactggg gcgtgcatca catcaagagc aacatgaccg ccgccagcac cggctttttg
      181 ccgaacgtcc ccggcgggtc ggaactggag caactgctcg gcgaactgtg gacgctcatc
      241 cagaacgagg ccgccacctt tgaaacggcc atcgagaatc tcaggacaca ggttgtagat
      301 cagatcacga cgctcccggc gggcgagatc gtcaagcgag tgatcgccat ctttctcgac
      361 gttctgctcg acacgattaa gaacatcgcg ctcgccgtta tagacatcct gcaattgttc
      421 ctccagggcg cgctcgatat gctggataag aagttggaca tccccgtcat ctcctggatg
      481 tacaagttga tctccggcga cgacctgagc atcctcgacc tcgcctgcct gatcgtcgcc
      541 atccctgcta ccgtcatcta caagctggcg aaaagtgaag tgcctttccc ggacgatgcc
      601 gccaccgacg ctcttatcca cgcgcccgat ttcgccacca tccgaaaact gctcggcggc
      661 caaaccgcga cgggtgagcg aacaccggca gaggccgcgc ttggcgcatc gtccgccgcc
      721 ggggtggtgc cggttgtctt cgacatcgcc gcctacttcg ccacgctcgg gctgatcccg
      781 ctcacgggat ggaaagcggc caacccgaac agcaagcccg cctacatcct ctacgccatc
      841 ctctatctcc cttacatcgc gcccggcatc cagctttaca ccccgcagac gtgggacgtg
      901 aaattgaacg agaggatcac cggcctcagc ctcctgaagt cgctcgcaga tgtggggctg
      961 tacaggtaca agaaaggagt ccctgaccag agtaagggtc ttgaagattg gtacaaagtc
     1021 tcgccgtatg tcgaggcggg catcaatgcc ttatgggaga taccagccat cgcgggcatt
     1081 gtgcggagtc aggacaccga ggccgtcatc ggctttctcg gcaacactgc tttcaacttg
     1141 agcggggtgc tcagcccgaa ggcggataac gattatgtgt ttgcggcgat attaatttgc
     1201 atgggcatct acggggaact cgaactggtg acaggcttga ccggcagcac agagcaactt
     1261 gcgctgacgt aagttcgcat cgctcccgga cgttggggcg tcgtagcggc ggagcgtacc
     1321 ccttacaaca taagaacggc atgcgcccga ccgcgaagca gcgaccttgc gacatgccgt
     1381 ttgacgcttt acagcgcggg gcgtcgaggc tttaaggttg caccctgatg tcgccgctct
     1441 ggtaattgac gttgccgaag atgtgcgccg agagcgggta gtcgcggcag ccgatcaccg
     1501 aactgttctt aggctggcgc acgcccacgt cgtccgtccg ttcggcgtcc ttggctatcc
     1561 aggtgagggt cgccccgttg tcgtcggcgc gctctgcgtc cttcggcacc caacttccgg
     1621 tcgtcccgtc gtagagcagc cacgtcagcc tgtcgcctgt cttgatgtca actccgctgt
     1681 cttcgaccac gagcagcacc cgccgccccg cgtagctctt gggcgcggcc tctctgaccg
     1741 tgccgcccat caccgccctg ttcttgtaaa cggtcagaca atcgaattcc gccttcatgt
     1801 agaagtccgt ggttgagcca gccaaacgcg ggtcgccggc tgccgccgca tcctgttccg
     1861 acattacgag ttggccgctg aaactcaaac gcccggtcgt cgtgcctttc gcgtccgccg
     1921 tggcgctgaa ctccacatac tttgcgaggc catccgccga aacgaacttg tagctgccgg
     1981 tagccgaggt tacccccgtc tgagcggtag ccggacgcaa gctcgcagaa ctcagcaagg
     2041 ccagtgccaa cgtcagggcg catgctatgc ttttcatcag ggtagctcct tggaaagtta
     2101 acagagtata gagggggcga gggcgattac ttatataacc gtccctgccg gaagtcaacg
     2161 gcgttagacc gtgttcgttc acagactcta cctctcatct acgaaaccat ctccgcgcta
     2221 tttccactca gttcggcctg cgacatccgc gctgtaattt tcccactccg gcgggatagg
     2281 cgcgcagccc gctgccggga gcctctactc cgccctttca tttcccggca tcaataaagc
     2341 ctccctgatc tctccgtctc gaatcgagaa ttcatagaga ggatggcgga tagcttcgat
     2401 gctttgattc ggataataga tcaaccctcc gccggtcggg atgccgatgc ctttctcgac
     2461 gccatcgagc aggcggacgg ttcttttaag cctgacccat tcctgcttct cgtcatgtgc
     2521 atctatgacg aggggaatta gcttgaacgt atggagcaac tcttcaagtg gctgcccccc
     2581 gtctatcaac ccgcatagtc cgagttggac cgctcccgcc gaagtcccta tcagggtagc
     2641 cccctcatag tatcttctga ttaacagttc tttgattccc gactcctcga ataccttcca
     2701 ccccgctgcg acatctcctc cggccagtag aataatgtct gcttcattca ggtaggcttc
     2761 atcctctgcc gggaaggaag agcggaccat tctacaatta tgaatcccga tgccttccat
     2821 cgccgcttcg aagatgagat agaactccgg cgcatcgccg ttggaagctc ctacgtaggc
     2881 agccttcgga gcatcattct caagaagttg gcgcagcgaa ttgaggaaga gaactccctg
     2941 attcttccag aacaagagct ggctatcggc aaagaggtag atcggctgga ttggcacatt
     3001 cataaccggg acaggttcgc gcgttggaga ttttgatggc atcgaggctc cgtcgaatca
     3061 gtcagaacgc cgcgcccgca gacaccattt tccctccacc ggattctcaa atttggacgg
     3121 gtgaattcaa cgcggatagg gctataatat caaatgttgc tgcgttagag tccactccgt
     3181 taacccgacc gctcgatagt aaccccttcg tcgttggaac gtctcatcat aatgaatgaa
     3241 gtttccccgc agcgggttaa ttagggcatg aagcggttat cgcgccatct ggaaaataga
     3301 cgtgatgttc agaataaata gattgcctct ctgcgcgttg gcggttctgc tgcttgcgtt
     3361 tcaatctacg gcacaacagg agattacaca ccaccaactc gcggccactt atgtgagcgg
     3421 ccacgagtac gcgagcggcg cattgacgct tcagaccgat ggaagatatt ccatggaagg
     3481 gggcagccac gacggcacgg agttctccga atccgggact tatgttctcg ccgggggcgt
     3541 cgtgcggttc actatcgaga ggtcggtcaa gagacgcaga ggcgtcgagg gagaaataaa
     3601 cctgctcgaa ccgcaggaga gtagagaagg gaaagagggg tcgggcagca actctactgc
     3661 gggcatcgcg agagagttca gcctgattcc gtttagatgg tcggagcgta tttatctgat
     3721 caaagaggac gacctgatca atttcgtcaa tgccgtcaat ctcggtctgg agccgcgcac
     3781 ggagctacgt tccgagaatt actacggcgc cttttatctt cgcagaggcg acgagttgaa
     3841 gagagcgagc gggcacccgt cgctccccgc gaaatggctc tcattcctgt taagcaagag
     3901 cgtgacggcc actgtgaccg ccattgagga gagccaaaaa ggatcattct cggcgagtag
     3961 tacggcgagg atcgacaagg gaagcaggga cggactcaaa gttggaatgc gtctgttaat
     4021 caatgaagag gagccgtcca cctcgctagg agcggagatc gtctctgtgg aagagaggac
     4081 ggcgaggata cgcatagcgc aggttaatgc ggaattaaag atcggcgacg tggtcagaac
     4141 cagagatgag ccgaagctcg acgagtatgg cgcactcagt tgggaggagg aaaaaaagcg
     4201 gctcgccaga ttcgccgcgc gcctgttgca gaggccggac gcacagggat acgttattgc
     4261 ttacgacgga aggcgcgcac gcgcgggcga ggcgttggcg cgtgccgaac gcgcaagagc
     4321 ctatttagag aaagagttga aaatggaatc ggggcgtatc gtagccgtgg acggcggtta
     4381 tcgagaagat caagctgtag agttatacgc cgttcgtagc ggaactaaac ccccggagcc
     4441 agccccgacg attaacccgg gcgaagtacg gaccaggcgc ggcaacgtga agccgggcag
     4501 cagccggaga taaattgaag agaaccgacc aacgggaaat ttacagaact taagggactg
     4561 acggttgccg gacgcgccgt tccacttacg aaaccgcttc cggaaacata gaccggcact
     4621 tcaggcacag gaagattggt gagactcatg cgccacagca cttcttgaac ttcttttcgg
     4681 agccgcaggg gcatggctcg ttgcgtccga ccttctgaga gactgccggg atagctccgc
     4741 cggagacgaa tcgccagtcg ccctcctcgc gctcgaagag ggaacgctcc ctgtgaagga
     4801 tggccgtgcc gtctttatct tcgtaacggg cttcaaaatt aacgtgcgcg cggtcgggcg
     4861 tggaggagtc ggagccgagg atggtgaggc cgagccagcg cgattcccgc gaccagcgac
     4921 cggcggattc gggatcaaat tgcctgcggg tctttgagtg attgctcttt ccgaggaaat
     4981 caattgcccc cgtggcgtaa gcactgtagc gcgctctcat gagctgctca ggggtcgagg
     5041 cttgaacctt accgtcgatg atgggttggc aacactgtgc aaattctccg ccgccgcagg
     5101 ggcactcctg tagatcaata tccaagtcat tacacctcga atgagtttgt cgcgaacgtc
     5161 ataatacaac aatagttgac agcctgttcc gggactcggc ctcgccatcg tcaggcaatt
     5221 catcgaagcg cagggcggga aggtgtccgt cgagaacgca aacggccacg gctctacgtt
     5281 tcgcttcacg attccctccc gaagcgggaa cagcgtaaga accgccaacc aaagccgtac
     5341 ctgttatgag agcgggtagc cgattattga tctcttatcc gggctaacac tttgccctcc
     5401 gcaggatgac agagatctgt atttgaatcc gggcttgtcg ccgggatggt acgcaaactc
     5461 tatcctcatt cccccttgaa tcttccccag ttcatataag attcttgggc aggtcaatcc
     5521 agactcatac tcactcagta cgattgagtc gtagacgttg atggtggtcg agagcattaa
     5581 gttgaactcc ccgtctaaat ccttcgggac atcaatgacg accttcggag agagggatgc
     5641 cccgctgtgc tcgctgccgt cgaggggggc tgacaggtta agggaactcc cggcggtcaa
     5701 ctcaagaacg ctgcccagat caaccctgac tttgttcctg gtgctgcccg gcgacaaaga
     5761 atttccggcc ttggcttcga cgtgaaggga tgaaaactca ttcgtcggat cgcagagatg
     5821 gcaacttaca actatcctct ccggaatgaa aatcccgccc gcacagagtt gaggagcgag
     5881 gttgattgtt atggctacct gcggctcttt ttccagagcc gcctgcatgg cttccaccac
     5941 cacgatatgt atgacgtgag gcgcgtcatg cttataggat gcggcgtcac accgtatgta
     6001 atcgcgcacg aagggggttg ccccgaaggc ttgaaaaaca ctttgcgccg cgtccagaga
     6061 gcgcctgtga atatcgagca gggtgaaccg tatctcggcg gggttgaaac ggctggtcag
     6121 gggaatagcc agaggcgcga aaggaccaca tccggcgtaa agtatctcta tcgtagtgtt
     6181 gggaaaccgc ctctgtgctt cgagtatcgc agcatgtatg ccgcgcagaa gtttagaggt
     6241 tctactgtag tccagtacgc aactggcggc gtctctgggg gagagggctt tcccgttcgg
     6301 aagcacggtt tcatgcgagt tatcagcgta acttgagtct tcatctattc cggttgacgc
     6361 ggagcataat gaatatagct ctgtcgccgc cacacgtaat ctgtcggcat cttcccgttg
     6421 agacaacaac tgatctgcaa tctgcttcaa ccggcgcgaa taattatctg ctggccgctc
     6481 aatgatgttc actggctttc caaagcgggc ggcttaacgc cgtggataac ccgcgagcac
     6541 ccgcgccgga cgaggcttta aagctccgcc cggcgcgggt gctcgcgttc atctcttggt
     6601 tatgcggctg gcggcactca acacaccgcc agccgcatga ataggagcac cagccgacgc
     6661 gcattctcaa acagcggctg agttagcact cgataggatc aacgtgcggg tcgccgcagg
     6721 atttcagcca gggcatgcaa gcgggctgag tctgccgcat tcttcgtcag gccagcgctc
     6781 ttctccaaag acggcgcgag gctcttgagt tttccgagtt cgcccggatt cagttgtgaa
     6841 ctctctgctc tctgaatcgc ctgccgcacg gctgcgatcc ggtcagaagg cagagcttgc
     6901 gaacgctcca gttgatcgac gtatgccttc gccacgatta actgccgagg ccactcgatc
     6961 ttctcctgat tctgcacatt cagttcagcc acgcgaaccg tcttcgccgc gtcgatctcg
     7021 ttctgcgtaa ggtgttgcgt cggggtcaac tcgaagatgt cgaggccgcg cgcgatctcc
     7081 gatgcgtaaa tgtgaccgtt gtaccagtaa gcagaccagg ctccgccgag cacgagcatc
     7141 ttcggatcga ccggaccccg gtcgaaatag gcaatctcaa ttggatggcc ggggtctgtg
     7201 aagtccatga gggagatgcc gccctgatac caggcttgca ccttgatgtc ccgaccggga
     7261 accggaagga gcgagccgtt gtgcgcaaca caattctcgc tgtcgccctg cgccgccggt
     7321 aatttgtagt agcttgcgaa gttcagcttg ttacccgtca ggcggaaaat cgcattcgcg
     7381 ccccacacgt tcggatcgtt cgcgcggcag cgcgcaccca atccgccgcc ccactcgtct
     7441 gtaaacacga ccttcttccc gtcattggag aacgaggccg agtgccagta ggcatagttc
     7501 ggatcgttca cggcgtctat gcgctttgga ttgacggggt ctttgatgtc gagcagaatt
     7561 ccatttcccg aacacgcgcc agccgcgagt ccgagcgaag agtagactgt aatatcgtgg
     7621 cactggtcgg tgggagacgg cttttccaat ccgttcttgc cgtgcgtgcc gccgttgttc
     7681 agaccgttca gagcacccgt cctcgggtcc ataaacactc tcggactcga cactacctga
     7741 gcattttgcg gagccgccaa cggcacctta atcacgtcaa tgcgaaagag cgcggtgttg
     7801 gggtctttgt ccggcggttc acccgaacac ccggccagct cttcgctctg acggacgaaa
     7861 gaggtgccgg agacgtagat gtagacgttg ttcttatcct tcggatcgac taccagtgta
     7921 tgcgtgtgcg agccgcgaca ggtttgcact gccgccacct gcttcggatt cctgatgtcg
     7981 gagatgtcga aaatcctgac tccgcgaaag cgttcttttt gcgccgccgg cagagtcggc
     8041 acgttctctt gtcccgcagc gggcggaggt tcgggcggaa aaccctgcgg actgcaatcc
     8101 agcctgccgt tcggcatctc caccgacata aagagcaggt tcttgtgaac cgacacgtcg
     8161 ccctgcccgc ccggacacac catcgaggtc agcaatctgg ccttcgccgg gtcggcgatg
     8221 tcatagatgc tgacgccgta aaagtttccc ataaacaggt ggttgccctg gaacgcgagg
     8281 tctgagttgg cgaaagcgag ttgggcaaga acgagttgga gaggcttcgg aatcttcgag
     8341 gcgtcgccca tgccgaggac gcccagcgtc ttttgcacct tcggattatc aggatcgtcg
     8401 gagccgagtt gaaacgcgtc cggctttttg agcagcatga gatgcctcat gcccatcgca
     8461 gcttcacccg cgtcgtaaat tcccggcgac agtttggcgc gcgggtcgtt ggcatccgaa
     8521 cctttcatcc ccgccgggag cggcggagcc gattcagggg caaagggatt cccaccggcc
     8581 tgctcggcgg gcttggtttc cggcgtcttc acgggttcgg gagacggcga cggcgaagcc
     8641 gtctgcgcca gcgtcccggc ggcaagacat gagcacaaaa caagggaagc tgttagtaag
     8701 cggaatgcga tagtgtgaac tcgactcatc gtttctcctc gacaggtttt tcacccaaca
     8761 tggtttgcat aatcctaatc tcggcgcgtt ggccgctgtc tacatccgtt gcgaaattga
     8821 acagttcggc atcctgcccc gcgcctgccg tatcaaacag atcctttacc ataatcaacg
     8881 caccgttgtg atgctgaatc atcccgttca aaaacagacg atcaaactcc gcgccctttg
     8941 cttttctcaa ggcgtccatc tgcttcgggc taagcattcc gggcatcagc gtgtgatggc
     9001 tcgacatgtc gtggccgggc atatccatgc cgtgcatttc atgcttcggc ggcgaaaccg
     9061 gctctcccct ggcttcgagc caacgtctca taaacttcat ttcgtctgac tgtgattggc
     9121 tgatacgcgc tccgagcaac cggagttccc tgttctccgt atgcgactcg attagcgcag
     9181 tcatctccac ggcttgcgcg tggtgcatga tcatgccctg catgaactcc acatccttcg
     9241 gcgaacgcgg cggcagtgtc gccctcgtcg atggcggcaa tgtttgggtc ggttgccccg
     9301 gagcaccggg ttgcaccaca acgggcggtt tagagtcagc ctgctgtgcg tagactggca
     9361 gcgataagaa acacatgaga gcgcaggcgg ccatgagcag gctttgcggc gcggcaacac
     9421 tggcgattgg gcgcggccta agcccggcac tgaaagtaaa cgactgcata agcgttgatt
     9481 gatcgagcat tgagcggtga tcttaccaca aataagagtg gcgataactc accggagccg
     9541 cccgcgcaaa gtgcgccgcg cgatgaacat aaacattccc gctacaggcg aatgttagac
     9601 gcgcgggcga agcgcgctgg aaaaagtagc ggcgtaaaat gagattgctc gaagagttgg
     9661 ctgcgttgct tcggttgcgt cgtatctttt tcgccgcgct taaattagaa tcgcgcttgc
     9721 ccgcaagctc acatcaggac ggttcacatg ctaaacaaaa cttttcgctc tctcaggaca
     9781 aactcgcgca ggcgggtgtt gaagctaaga gacacaaacg ctgctgcact attgatcgcg
     9841 ccgctggccg cgacgctcat gatgtgcgcg gcgacagttg cgtctgccga agactggccg
     9901 cagtttcgcg ggccgaacgg gagcggcgtg acggcatcgc cggggctgcc ggaagtgttc
     9961 ggccctgaga agaatgtggt ttggaagacg ccgccgccgc ccggccattc ttcgccggtg
    10021 ctgacgcgag atcgcgtgtt catcaccgcg tacgccaaag aaaaagccat cggcactcag
    10081 caaacggtag cgggcactaa ggggaacgcc gccgagaaga atcataagct cctggttatc
    10141 tgtctcgacc gccagacagg aaagctgctg tggcaacgag aagtgccgcg ctcgcgcgag
    10201 gggcgattgc agaatgtcaa cgaccctgcc tcgcccagcc ccgtcacgga cggcacgaac
    10261 gtctacgtct tttttcagga gttcgggttg gtttcttacg accgcgctgg caaggagcgt
    10321 tggcggctcc cgctcggacc cttcaatatg ttctacggat tcggcgcgtc tcccattctc
    10381 gtcgatgaca aagtgatcct gcccgtcgat caggacagtc ccagttcata tctgatcgcg
    10441 gttgataaga acaacgggcg cgtgcgttgg aaggttgaac ggcctgcggt catctctggt
    10501 tactcgacgc cgattattta ccagccgaag cagggtgcga agcagatcgt tattccagag
    10561 tcgttccagc tctcggctta ctcggtcgcg gacggcaaca gagtttggtg ggtgcgcggc
    10621 ctggcgtgcg agatgaaatc cattgccagc agcgacgggg aatatgtgta catcaacggc
    10681 tggggcttcc cgcagaatca acccggaaac cagatcagca caattccctt tgaagagtcg
    10741 tgggcgagat acgataagaa taacgataag ttaatcgcga agtcggaggt gacgggcgcg
    10801 gagaagatgg acaagatgtt ggccgccgcc ttcgaggcgt tcgatatgga ccgcgatgag
    10861 aagttgaacg cgaaggattg ggaagtgttt cgcggcatga tggcttcgga gaacggcctg
    10921 ctcgcaatca aactgggagg acaaggcgat cagacagcga ctgcgatccg ctggcgctat
    10981 cagaaaccgg tgccgcaggt tccttcgacg cttctctaca aaggcgtcct gtacatggtc
    11041 aatgacagcg ggatcatgct ctcgttcgat ccggcgaccg gcaacgtcat caagcaaggc
    11101 cgcctgcatg gcgctattga taaatacttc gcgtcgccgg tggcggctga cggcaaggtg
    11161 tttctggttg ggcagggcgg tcaggtgtcc gtgttgaagg ccgcaggcga ttgggaggta
    11221 ctcgcggtaa acgagttgga cgatgaggtc ttcgcaacgc cagcgattgc agacgggcgg
    11281 gtttacatca ggactcgcag cgcgctttat agttttggca aata
//
LOCUS       DASWNS010000326        10733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_13517, whole genome shotgun sequence.
ACCESSION   DASWNS010000326 DASWNS010000000
VERSION     DASWNS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10733
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13517"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..3498
                     /locus_tag="VGB76_11440"
     CDS             235..3498
                     /locus_tag="VGB76_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099553.1"
                     /translation="MLKFRWPQVWALALLVLICATLPSHLAAQQQTPTPTPTATPEER
                     YDPAKDAGSPAKNTGTTAAAPAKESNPFERLEWRSIGPANMGGRTADVEGVAGDPDIV
                     YVGTASGGIWKTTNGGQTWRPIFERQGTISVGDLALEPKNPEVVWVGTGESNVRNSVS
                     FGDGVYKSTDGGRTWQHMGLRDTRHISRIVINPRNPDVVYVGALGHAFGANEERGVFM
                     TTDGGRTWTKTLYLDAEHGISDLDIDPVNPNVLYAAMWQFRRTPWTHRSGSERGGVFK
                     SIDGGRTWNKLTGGLPKLIGRIGVKVAPSNPNVVYAITESKEGTLYRSDDAGENWRRV
                     SNQANIVSRGFYYTDVRVDPTNADRVYAIASTLFVSIDGGRTFRSITGRTHIDYHALW
                     IDPVDPRRIWQGQDGGVAVSYDRGETWEYVNNMPLGQFYQLHADNRQPFYYVMGGLQD
                     NGSWAGPSRTREPAGILNDDWRMISFGDGFYTLNHSDDPELYISESQGGGIVRTDMRN
                     REQQAVTPYADDLGGPASDQRFRFNWNSPIIPSPHDKNTIYLGGNVVFKSTDFGKSWT
                     AISPDLTTNDRTKQGDAGGPVALENTGAEYYTTVISIAESPLRAGHLWAGTDDGQLQA
                     TTDGGKRWTNLTKNVPGLRDNSPVSHIEPSRADADTAYVAFDRHLLDDLRPYIYKTTD
                     GGRTFRNITGNLPANAYVWVVREDPKNTNLIYAGTELGLYASYTGGNSWQILELKNLP
                     HVAIHDIIVHPRDNDLILGTHGRSIWIFDDATPIQQMNDTVRAADLHMFDIRPALRFT
                     TRFTRYGIGDKPFTGQNPPYGALITYHLKAKPDEKTKVRMEIRDSGGKLVFEETRVPK
                     ERGLNRYAWNLRYGGAQVRRPPTTEETPFGGGARGPQVLPGVYTVKLFVGDKSIERRV
                     EVRLDPTVSVPASDLQMTHDMSLKLRDMQSTTNTMLRALDSIKGQLQFIERTVNERLS
                     EPPKDLTTALTDNLKAVDKVLDTLSRPQSEGLGYNPGRAQLIDEIGGLFFTIDATNAA
                     PTPAQREYYNTLQTRFRTTLAEANKFFTASVPKLNETLRRHDAPTVMVGKPLEIPAP"
     gene            3580..5052
                     /locus_tag="VGB76_11445"
     CDS             3580..5052
                     /locus_tag="VGB76_11445"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0099554.1"
                     /translation="MHKFKIRFVARLLLVVSLTAQVSPAARAQGSASLNAEQRVLREI
                     FQELIEINTVDPEGNVTRAAEAMAARLRAAGFAAEDVRVVVPAGNAKKGNLVARYRSP
                     KASGKPLLLLAHIDVVEARREDWSDGLDPFKLTERDGYYYGRGTMDDKSMAAIFIANL
                     IRFRRENFQPDRDIIVALTTDEESGDFNGVEFLLKEHRALVDAEFGINEGGRGYLKGG
                     KPLLNAVQASEKVYQSFRLEVRNKGGHSSLPVKDNAIFRLAAGLDRLAKFDFPVNLNE
                     VTRSYFARMSKLEHGQIAADMKAVSEVRPPRAGATAATRPEAQAAIARLSESPYYNAL
                     MRTTCVATRLEGGHADNALPQTARALINCRILPQETAAEVERTLRRVLADEQISIASI
                     ALSKPSPPSPLRPEIIVPIEQITHAMWPDVPVVPIMGTGATDSLFFRQVGIPVYGVSG
                     IFDDIDDNRMHGRDERIAAKALYDGQEFLYRLTRALSSVK"
     gene            complement(5089..6489)
                     /locus_tag="VGB76_11450"
     CDS             complement(5089..6489)
                     /locus_tag="VGB76_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0099555.1"
                     /translation="MEAASRAAQKRSSFEMIRRRRDELRLRPRAPKFGHRLTGAVNSL
                     CAIIIPMRHLRSRTIFILLGVWTLIGLTFASLSFFGTLAISDPRRVNLVGLFVWNLLA
                     FYLWALLTPLIARLARRYRLDRQHWPRSLFLVHLPASLLFPALHLCLYLSLFWLVGGV
                     LTKEYPTYLSLYRYQLFSNLSVTVSIYWLVLIAINALDYYQSFRVAEVKASELRAQLA
                     QAQLRALKMQLHPHFLFNTLNSISALLHRDAEAADQMVARLGDFLRLTLENSGDQEVT
                     LEQELEFLRCYLEIEIVRFHDRLSVETEIEPQTLQARVPNLILQPLVENAIRHGISRQ
                     SAPGRLAIRAARREGRLLLQVEDNGPGLSLISEGGAIAPVAATHTTATPARTLTGGVG
                     LANTRARLQHLYGAEHRLELTRATPRGMLVTLEIPFQLEAAPGWADEEAQQGAHEEAQ
                     TAHYEAEPDQINHAVG"
     gene            complement(6624..7073)
                     /locus_tag="VGB76_11455"
     CDS             complement(6624..7073)
                     /locus_tag="VGB76_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0099556.1"
                     /translation="MMKDSALLERREINLAGDPRTGSSNSINGNNAVANRLALHDNTL
                     PARMEMLKNVALNLLKEVQVLSTLEASDILRGIQFYDEVERFERELITRALDLTGGHQ
                     VRAARLLGLKVTTLNSKVKRYRIPLPHDEAKKPQSPQDAGPFPKPVV"
     gene            complement(7400..8224)
                     /locus_tag="VGB76_11460"
     CDS             complement(7400..8224)
                     /locus_tag="VGB76_11460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099557.1"
                     /translation="MKRIKSVLPLLLLLTLHGATQAQTKYVAVDNSARSSSPVGASTG
                     AASRTPDADSINNTGCVYADAGEYEKAIEQFKLAIALKPQLSEAHNNLGYAYALSGRH
                     AEALAALKEATRLNPASSRAQFNLGRVYMDTEQPKAALKHLQQSIALEPDFAEGHNQL
                     GVVYDRLGRHREALKSLLEATRLNPRSLSALNNLGVVQSNAKQYDAAIKTLRGALELD
                     PNFAQAQYNLGSVYAMLKDKRAALELHTTLKQLDAELGRLLYGEIYRDKILDVNQK"
     gene            complement(8613..9383)
                     /locus_tag="VGB76_11465"
     CDS             complement(8613..9383)
                     /locus_tag="VGB76_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0099558.1"
                     /translation="MNTIRTLIVDDELLARERIKRFLASERDIEPLGECADGREAVAA
                     INALKPDLVFLDIQIPELDGFGVVKAVGVEQMPAVIFVTAYDRYALRAFDVNALDYLL
                     KPYNRERFRKAVERARAQLSNGARGKLNERLLSLLENLKTEHRHLERLMIKSSGRVFF
                     LRTEEIDWIEAEGNYLRLHVGHERHLLRETMNRLASKLDPDKFLRIHRSTLVNIERIK
                     ELQPLFSGDYVVILRDGKQLTLSRSYRDKLLELFEKSS"
     gene            complement(9568..9723)
                     /locus_tag="VGB76_11470"
     CDS             complement(9568..9723)
                     /locus_tag="VGB76_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099559.1"
                     /translation="MTASAAFMLARWQPYAARYRLRLVPRQQVEPEASITLHPRHVIR
                     MTLSEHA"
     gene            complement(9795..10133)
                     /locus_tag="VGB76_11475"
     CDS             complement(9795..10133)
                     /locus_tag="VGB76_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099560.1"
                     /translation="MNEPEDFFEDIIGEEEAFEETDEFEDGEDGDDDEVPYEVRTAVM
                     QKNELLAILSLKISAEGGRIVRVDPRQRVPSAQTYEDAEAATHWFNRSLATSRRNGWH
                     VIYDGEPSFG"
     gene            complement(10175..>10733)
                     /locus_tag="VGB76_11480"
     CDS             complement(10175..>10733)
                     /locus_tag="VGB76_11480"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0099561.1"
                     /translation="ADVLAVIERLAGQDFTMVQLATDTPLHGSDYQDVHRDTPPLFPE
                     TGRETPPYQLAVNFPLVEVTHENGPFEVARGTHLLPKEEALKLIETGERKLEPLTMKL
                     GDVMVRDVRALHRGTPNRTHEPRPMVVVGYSRRWLFRPEVTIHIPRAVHETLSERARQ
                     MLRFNPVVESLDDLPETEIYQAFAY"
BASE COUNT         2099 a         3213 c         3366 g         2055 t
ORIGIN      
        1 cgagtgatgg gtgacaagtg agtagcaaga aacaagacaa gtttaaagat aaggttccag
       61 ctttaacttg tcaccccgtc acttgtcacc cgtcacccgt cactcgtcac tgctcttgga
      121 aactttccgc cgcgctttct cgaatctagc gatagacgcg caagcaggag ttagtttatc
      181 ttttcagttc cattctgttt ttgattcgag tctcaccgtt caggagtcgt tttcatgctg
      241 aaatttcgct ggcctcaagt gtgggcgtta gccctgctcg tccttatctg cgcaactttg
      301 ccttcccatc tcgcggcgca acaacagacg ccgaccccca cgccgacggc gacgcccgaa
      361 gaacgctacg acccggcgaa ggatgcgggc agccccgcga agaacaccgg cactaccgcc
      421 gccgcgcctg cgaaggagtc caaccctttc gagcggctcg aatggcgcag cattggtccg
      481 gccaacatgg gcgggcgcac ggcggacgtg gaaggcgtgg cgggcgaccc cgacatcgtc
      541 tatgtcggca cggcttcggg cggcatctgg aagaccacga acggcggcca gacgtggcga
      601 cccatcttcg agcgacaggg cacgatctcg gttggcgatc tcgcgctcga accgaagaac
      661 ccggaagtgg tgtgggtcgg gacgggcgaa tcgaacgtgc gcaacagcgt ctcgttcggc
      721 gacggcgtct acaagtcaac cgacggcggg cgcacctggc agcacatggg cttgcgcgac
      781 acgcgccaca tctcgcgcat cgtcatcaac ccgcgcaacc ccgacgtggt ctatgtcggc
      841 gcactcggcc acgccttcgg cgcgaacgag gagcgcggcg tttttatgac gaccgacggc
      901 ggcaggactt ggacgaagac gctctacctc gacgccgaac acggcatctc cgatctcgac
      961 atcgaccccg ttaacccgaa cgtgctctat gccgcgatgt ggcagtttcg ccgcacgccc
     1021 tggacgcacc ggagcggcag cgagcgcggc ggcgtcttca aatcaatcga cggcgggcgc
     1081 acctggaaca agttaacggg cggcttgccg aagctcatcg ggcgcatcgg cgtcaaggtc
     1141 gcgccgagca atccgaacgt cgtctacgcg atcacggagt cgaaggaggg cacgctctat
     1201 cgttcggacg acgcgggcga aaactggcgg cgcgtctcca atcaggccaa catcgtctcg
     1261 cgcggttttt attacacgga cgtaagagtt gacccgacga acgccgaccg cgtctatgcc
     1321 atcgcttcga cgttgttcgt ctcgattgac ggggggcgca cctttcgttc catcaccggg
     1381 cgcacgcaca tcgactatca cgcgctctgg atcgaccccg tagacccgcg ccgcatctgg
     1441 cagggacagg acggcggcgt ggccgtctcg tatgatcgcg gggagacgtg ggaatacgtc
     1501 aacaacatgc cgctcggcca gttttatcaa cttcacgccg acaaccggca gcccttttat
     1561 tacgtgatgg gcggcttgca ggataacgga agctgggctg gaccaagccg cacgcgcgaa
     1621 cccgccggaa tcctgaacga cgactggcgc atgatcagct tcggcgacgg cttctacact
     1681 ctcaaccact cggacgaccc ggaactctac atctccgagt cgcagggcgg cggcatcgtc
     1741 cgcacggaca tgcgcaaccg cgagcagcag gccgtcaccc cttatgcaga cgatctcggc
     1801 ggtcccgcct cggatcaaag gtttcgcttc aactggaact cgcccatcat cccttcgccg
     1861 cacgacaaga acacgatcta tctcggcggc aatgtcgtct tcaagtcaac ggacttcggc
     1921 aaaagctgga cggcgatcag ccccgacctg acgacgaacg acagaacgaa acagggcgac
     1981 gcaggtggtc ccgttgcgct tgagaacacg ggcgcggagt attacacgac cgtcatcagc
     2041 atcgccgaat cccccttgcg cgccgggcac ctctgggcgg gcacggacga cgggcaactg
     2101 caagcgacga ccgacggcgg caagcgttgg acgaacctga cgaagaacgt ccccggactg
     2161 cgcgacaact cgcccgtctc gcacattgag ccttcccgcg ccgacgccga cacggcctac
     2221 gtcgccttcg accgtcacct gctcgatgat ctgcgtccct acatctataa gacgaccgat
     2281 ggcgggcgca ccttccgcaa catcaccggt aacctgcccg cgaacgccta cgtctgggtc
     2341 gtgcgcgaag acccgaagaa cacgaatctg atctacgccg gaaccgaact cggcctctac
     2401 gcttcctaca cgggcggcaa ctcgtggcag attctcgaac tcaaaaatct gccgcacgtc
     2461 gccatccacg acatcatcgt ccacccgcgc gacaacgatc tcatcctcgg cacgcacggg
     2521 cgcagcatct ggattttcga cgacgccacg cccatccaac agatgaacga cacagtgcgc
     2581 gccgccgacc tgcacatgtt cgacattcgc cccgcgctcc gtttcaccac gcgcttcacg
     2641 cgctacggca tcggcgacaa gcccttcacc gggcagaacc cgccctacgg cgcgctcatc
     2701 acctaccatc tcaaagcgaa gcccgacgag aagacgaaag tgcgcatgga gatacgcgac
     2761 tccggcggga agctcgtctt tgaagagacg cgcgtgccca aagagcgcgg cctgaatcgc
     2821 tacgcttgga acctgcgcta cggcggcgcg caggtgcgcc gtccgcccac caccgaagag
     2881 acgcccttcg gcggaggcgc gcgtggtccg caagtgctgc ccggcgtcta caccgtcaaa
     2941 ctcttcgtcg gcgacaagtc aatcgagcga cgcgtcgagg tgcgtctcga cccgacggtc
     3001 agcgtgcccg cgtcggattt gcagatgacg catgacatga gcttgaaatt gcgcgacatg
     3061 caatccacga cgaacacgat gctgcgcgcc ctcgacagca tcaaaggtca actgcaattc
     3121 atcgagcgca cggttaatga gcgtctcagc gagccgccga aagatttgac gacggcgctc
     3181 acggacaatt tgaaggcggt tgataaagtg ctcgacacgc tctcgcgccc gcaatcggaa
     3241 ggactcggct acaatcccgg gcgcgcgcag ttgatcgacg agatcggcgg cctgttcttc
     3301 accatcgatg cgacgaacgc cgcgcccacc ccggcgcagc gcgagtatta caacacgttg
     3361 cagacgcgct tccgcaccac gctggcggag gcaaacaagt tcttcaccgc gtccgtcccg
     3421 aaactgaacg agacgctccg ccgccacgac gcgccgaccg tcatggtcgg caagccgttg
     3481 gagattccgg caccatagaa gagcaaggat gaagaatgaa ggatgaagga tgaagaaaag
     3541 catcgaagtt acataggctt tcgtggtgag aaaagattca tgcacaaatt caaaattcgc
     3601 tttgtcgcga gattgctgct cgtcgtgtcg ctgacggcgc aggtgtcgcc cgccgcacgc
     3661 gcgcagggca gcgcgtcgct caatgcggag cagcgcgtgt tgcgcgaaat ctttcaggag
     3721 ttgatcgaaa tcaacacggt agacccggag gggaacgtga cgcgggcggc ggaggcgatg
     3781 gcggcgaggt tgcgcgcggc gggctttgcg gcggaagacg tgcgcgtcgt cgtgcctgcg
     3841 ggcaacgcga agaagggcaa tctcgtggcg cgttatcgca gcccgaaggc gtcggggaag
     3901 ccactgctgc tgctcgcgca tattgatgtc gtcgaagcgc gcagagagga ttggtcggac
     3961 ggactcgacc cgttcaagct caccgaacgc gacggctact attacgggcg cggcacgatg
     4021 gacgataaat ctatggccgc gatcttcatc gccaacctca tccgcttcag acgcgaaaat
     4081 tttcagcccg accgcgacat catcgtcgcc ctgacgaccg atgaagagtc gggcgatttc
     4141 aacggcgtgg agtttttact gaaagagcat cgcgctctcg tcgatgcgga gttcggcatc
     4201 aacgaaggcg ggcgcggtta tctcaaaggc ggcaagccgc tcttgaatgc cgtgcaggcg
     4261 agcgagaagg tctatcagag tttccgtctc gaagtgcgaa acaagggcgg gcacagttcg
     4321 ctccccgtca aggacaacgc catctttcgt ctcgccgccg gactcgaccg gctcgcgaag
     4381 tttgacttcc ccgtcaacct caacgaagtg acgcgctcct acttcgcgcg catgtcgaag
     4441 ctcgaacacg ggcagatcgc cgcagacatg aaggccgtct ccgaggtgcg cccgccgcgc
     4501 gcgggtgcga cggcggcaac gcgccccgaa gcgcaggctg ccattgcgcg cttgtccgag
     4561 tcgccttatt acaacgcgct catgcgtacc acctgcgtcg ccacgcggct cgaaggcggc
     4621 cacgccgaca acgccctgcc gcagacggcg cgcgccctca tcaactgccg catcctcccg
     4681 caagaaaccg ccgccgaagt agagcgcacg ctccggcgcg ttctcgccga cgagcagatc
     4741 agcatcgcct ccatcgccct ctcgaagccc agcccgcctt cgccgctcag gccggagatc
     4801 atcgtgccga ttgaacagat cacgcacgcg atgtggccgg acgtgcccgt cgtgccgatc
     4861 atgggcacgg gcgcaaccga tagtctcttc ttccgtcagg tcggcatccc cgtctacggc
     4921 gtctccggca tcttcgacga catagacgac aaccgcatgc acggcagaga cgaacgcatc
     4981 gcagccaaag ctctctacga cgggcaggag tttctctacc gcctgacacg cgcgctctca
     5041 tccgtcaaat aatcttttag ttggtagttt cccgtcccgg cgtgcgcctc agccgaccgc
     5101 gtgattgatc tggtctggct cggcttcgta gtgtgcggtc tgtgcctctt cgtgtgcgcc
     5161 ctgttgcgcc tcttcgtctg cccaaccggg cgcggcttca agctggaagg gaatttcgag
     5221 cgtgacgagc atgccgcgcg gagtggcgcg tgtgagttcg aggcgatgct ctgcgccgta
     5281 gaggtgttgg aggcgcgcgc gtgtgtttgc cagcccgacg ccgcccgtga gagttcgtgc
     5341 cggtgtcgcc gtcgtgtgag ttgcggcgac gggtgcaatc gcgccgcctt cgctgatcag
     5401 ggagaggccg ggaccattat cttcgacctg gaggaggagt cgcccttcac ggcgcgcggc
     5461 gcgaatggcg agacgccccg gcgcgctctg gcgcgagatg ccgtggcgga tggcgttttc
     5521 gacgagcggt tgcaggatga gattcggcac gcgcgcttgg agcgtctgcg gttcgatctc
     5581 ggtttcgacg cttaagcggt cgtgaaaacg cacgatctct atttcaagat aacaacgcag
     5641 aaattccaac tcctgttcga gcgtcacctc ctgatcgccg gagttctcca gcgtcaggcg
     5701 caggaagtct ccgaggcgcg cgaccatctg gtcggcggct tccgcgtccc ggtgcagaag
     5761 cgcggagatg gagttgagcg tgttgaagag aaagtgcggg tggagttgca tcttgagcgc
     5821 gcggagttgc gcctgcgcga gttgggcgcg aagttccgat gcctttacct ccgcgacgcg
     5881 gaaactctgg tagtagtcga gcgcgttgat ggcgatgagc acgagccagt agatcgaaac
     5941 cgtgaccgag aggttggaga agagttggta acgatagagc gagaggtagg tcgggtactc
     6001 tttggtgagc acgccgccga cgagccagaa gagcgagagg tagagacaga gatggagcgc
     6061 ggggaagagc aggctcgcgg gcagatgcac gaggaagagg ctgcgcggcc agtgctggcg
     6121 gtcgaggcgg tagcggcgcg cgagccgtgc gatgagcggc gtcaggagcg cccagagata
     6181 gaaggcgagc aggttccaga cgaagaggcc gacgagatta acgcggcgcg ggtcgctgat
     6241 ggcgagcgtg ccgaaaaaag agagactggc gaacgtgagg ccgatgagcg tccagacacc
     6301 gagcaggata aagattgtgc gtgaacggag atgtcgcatc ggaattatta tcgcacacag
     6361 agaattaacc gcgcccgtca acctgtgccc gaacttcgga gcgcgcgggc gcaagcgaag
     6421 ttcatctcta cggcggcgaa tcatctcgaa gcttgaacgc ttctgcgcgg cgcggcttgc
     6481 agcttccatc ttatctcagg gcgaacaaaa ttcgggcaga ggagttatcc cctgccctga
     6541 atggtgttct tcgttgcggt aaggatttgc tcgtggctcc cgaatacttg agacgcgctt
     6601 gccgcgctgg cgatctaacg gcgtcagacg acgggcttcg ggaaaggtcc ggcgtcctgc
     6661 ggcgactgcg gcttcttggc ttcgtcgtgc ggcagaggaa tacggtagcg tttcactttg
     6721 gagttgagcg tcgttacttt cagaccgaga agacgcgcgg cgcgcacctg atggccgccc
     6781 gtcagatcga gcgcgcgcgt gatcagttcg cgctcgaagc gttcgacttc gtcgtagaac
     6841 tgtatgccgc gcaggatgtc cgacgcttcg agcgtgctca gcacctgcac ttctttcaac
     6901 agattgagcg cgacgttctt gagcatctcc atgcgcgcgg gcagcgtatt atcgtgcagc
     6961 gcgagacggt tagcgacggc gttgttgccg ttgatgctat tgctgctacc ggtgcgcggg
     7021 tcgcccgcaa ggttgatctc tcggcgttcc agcagggctg agtctttcat catttctctc
     7081 ctcaacttcc tctctaaact accgtccaat caagcacgac cgggaagctt gcgccgcgct
     7141 tatcaactta acggcttcag tttagccgcc tcccggcggg cggcaacgaa aatgtgacag
     7201 gccgcaaatt cctgtgctga acggcgcgtg gccggggatg aaacttcatg cgtcccgaag
     7261 aaaactccga gaagaacccg tgaaccctga agcagacgct tcctccggcg actccccctc
     7321 gcgcccgccg ccgcgtgtct taaacgtctt gcctgtcaac caaagtggag gcgcgtgcgg
     7381 cgcgcgttcg ccgcattttt cacttctggt tcacgtcgag aatcttgtcg cggtagattt
     7441 cgccgtagag tagtcgtccg agttccgcgt cgagttgttt gagcgtcgtg tgcagttcga
     7501 gcgcggcgcg tttatctttc aacatggcgt agaccgaacc gaggttgtac tgcgcctgcg
     7561 caaaattcgg gtcgagttcg agtgcgcccc ggagcgtctt gatagccgcg tcgtactgct
     7621 tcgcgttgct ctgcacgacg ccgagattgt tgagcgcgga gaggcttcga ggattgagcc
     7681 tcgtcgcttc gagcaaagat ttgagagctt cccggtggcg tccgaggcgg tcgtagacca
     7741 cgccgagttg attgtggcct tcggcgaaat cgggttcgag cgcgatggac tgttgcagat
     7801 gtttgagcgc ggctttgggc tgctccgtgt ccatatagac gcgtccgaga ttaaattggg
     7861 cgcgtgaact ggcggggttg agccgcgtcg cctctttgag cgcggccaac gcttctgcgt
     7921 gccgaccgct gagcgcgtaa gcgtagccga gattgttgtg cgcttcggag agttgcggct
     7981 tgagggcgat ggcgagtttg aactgttcga tggctttctc atattcgccc gcgtcggcat
     8041 agacgcagcc tgtgttgttg atcgaatccg cgtcgggcgt gcggctcgcc gcgcccgtcg
     8101 atgctcccac gggtgaagac gaacgggcgg aattatctac ggcgacatat ttggtctgcg
     8161 cctgagttgc gccatgcaga gtcaagagga gcaagagcgg caagactgac ttgatgcgtt
     8221 tcatgttcac ctcctcacgc cagctcgaag gctctcgcgg gctgacgctg gttcgggcaa
     8281 aaagaaaaag acacgcatcg ccgtccggtg ggcattccgg cgatgcgtgt cttctgtgat
     8341 ctcgcgtgcg acgacgactc tgttctttta ccagcgtcgc ctgcaactaa aaatcattcc
     8401 cgcgcccgcg tcccgccgcc gccaaggtct cccgcaaggc tacagcaaga cgcgggcgca
     8461 agaatgtatg gcgtgagttt ggaccttgcg gcgcgagccg acaatgaaaa tgtgacgagg
     8521 ggcgagatgt tgatgacgaa cgggcggatt cctgtgacga aagaggcgag acgaaagaga
     8581 tgggcggcgc ggtttaaaga acgacgcgcg ggtcaggagg atttttcaaa cagctccagt
     8641 agtttgtcgc ggtaactgcg gctgagggtg agttgtttgc cgtcgcgcag gatgacgacg
     8701 tagtcgccgc taaagagcgg ttgcagttct ttgatccgtt cgatgttcac gagcgtcgaa
     8761 cgatgtatgc gcaggaattt gtccgggtcg agtttggagg cgagccggtt catcgtctcg
     8821 cgcaggagat ggcgttcgtg gccgacgtgc aggcgcaggt agttgccctc ggcctcgatc
     8881 cagtcaatct cctcggtgcg gaggaaaaag acgcgcccgg aggatttgat catgagtcgc
     8941 tcaagatggc gatgttcggt cttgagattt tccagcagtg ataggagccg ctcgttgagt
     9001 ttgccgcgcg cgccgtttga gagttgcgcg cgggcgcgtt ccacggcttt gcgaaaacgc
     9061 tcgcgattgt agggcttcaa cagatagtcg agcgcgttca cgtcgaaggc gcgcagggcg
     9121 taacggtcgt acgccgtgac gaagatgacg gcgggcatct gttcgacgcc gaccgctttg
     9181 acgacgccga agccgtcgag ttcgggaatc tgtatgtcga ggaagacgag atcgggtttg
     9241 agcgcgttga tcgcggcgac ggcctcgcgc ccgtcggcac actcgccgag cggttcgatg
     9301 tcgcgttcgc tcgccagaaa acgcttgatg cgctcgcgcg cgagcaattc gtcgtcgacg
     9361 atgagggttc tgatggtgtt cacacgttcc cagcctctgc tctaggtggc ttgcccgaag
     9421 aaggattaca gcgaaacgtg ccgctcgaag aggttgacgg caatgcttta tataacgccc
     9481 cgcaagcgag tttgtttcac acaaaagcca tgtttgcggc ttagaagcct gcggagcgcc
     9541 atttggttgc tatctttatt cgagatttca ggcgtgctct gagagcgtca tgcggattac
     9601 gtggcgtgga tgcagcgtga tcgaggcttc gggttcgacc tgttgacggg gcacgaggcg
     9661 caggcgatag cgcgcggcgt aaggctgcca gcgcgcgagc atgaacgcag ccgaagcggt
     9721 catcgtctcg ccgaaggcgt tgatgcgctg gcgatggaac gcaggttgag cgagaacgcg
     9781 ctcttgaatt actttcagcc gaaggaaggc tcgccgtcgt agatgacgtg ccagccgttg
     9841 cggcggctgg tggcgaggct gcggttgaac cagtgcgtgg cggcctctgc gtcttcgtag
     9901 gtttgggctg agggcacgcg ttgtcgcgga tcgacgcgca cgatccggcc accctcggcg
     9961 gaaattttca ggctcaggat cgcgagcagt tcgttcttct gcatgacggc ggtgcgcacc
    10021 tcgtaaggca cttcgtcatc gtcgccgtcc tctccgtctt caaattcgtc cgtctcttca
    10081 aacgcttcct cttcgccgat aatatcttca aagaaatctt cgggttcatt cataatctca
    10141 aaaatcccac tttcaaatta actttcgatc tctactaata cgcgaacgct tgataaatct
    10201 ccgtctccgg cagatcgtcg agcgattcga cgacggggtt gaagcgtagc atttgccggg
    10261 cgcgttccga cagcgtctcg tgcacggcgc gcggaatatg gatcgtcact tcgggacgaa
    10321 acagccagcg gcggctatag ccgaccacga ccatcgggcg cggctcgtgg gtgcgattcg
    10381 gcgtgccgcg atgcagcgcg cgcacgtcgc gaaccatcac gtcgccaagc ttcatcgtca
    10441 aaggttccaa tttccgctcg cccgtttcga tgagcttgag cgcttcctct ttcggcagca
    10501 ggtgcgtgcc gcgcgccact tcgaaggggc cgttttcgtg cgtcacttcg acgagcggga
    10561 aattgactgc gagttgatag ggcggcgtct cgcggcctgt ctcggggaag agcggcggcg
    10621 tgtcgcggtg cacgtcctga tagtcggagc cgtggagcgg cgtgtcggtc gccagttgca
    10681 ccatcgtgaa atcctgtccg gccagacgct cgatgacggc cagcacgtcc gca
//
LOCUS       DASWNS010000327         6244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_195792, whole genome shotgun sequence.
ACCESSION   DASWNS010000327 DASWNS010000000
VERSION     DASWNS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195792"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /gene="nusA"
                     /locus_tag="VGB76_11485"
     CDS             complement(<1..1578)
                     /gene="nusA"
                     /locus_tag="VGB76_11485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY0099562.1"
                     /translation="MSASIAQSIDALCKEKGVDREIVIEAMKEAVRAAARKQFKSGED
                     IQVEWSSENGFELFASKVVVDEVTNPGKELSLEEAREMAGDEVDVGDELQLPLPMEDM
                     GRIAAQTAKQILFQKVRDAERQNIYEQYIDKVGDLVNGYVKRFERGDIIVDLGTVEAI
                     LYRKEQSRGEQWGQGERIRVVINDVAKEAKGPQVEVSRTSPELLRRLFEMEVPEIYDE
                     TVIIKSAVREPGERAKIAVASNERDVDPVGACVGMKGSRVQAIIRELRGEKIDIIEWS
                     DEPSVFAANALSPAKVNQVRITDIERRQMEVIVNEDQLSLAIGKRGQNVRLATKLVGW
                     NIDIRSEEELKKEVAEQMGALIASGGAVPLTALEGVNAATTAALAERGIEDIDTLAGT
                     SLDDLTEYLDISLDEAQAILDSAQRVVAARERSKQDDGEGANTAEAEAAAGELADAVD
                     DATPDDALEQHAVSAELDPTIDPGEVEPSDAMIAEGYDEAVRGGTPFRAESEILAAES
                     ADPVAASEVEEMSVEELV"
     gene            complement(1689..2213)
                     /gene="rimP"
                     /locus_tag="VGB76_11490"
     CDS             complement(1689..2213)
                     /gene="rimP"
                     /locus_tag="VGB76_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY0099563.1"
                     /translation="MEASEIEGRIREIVERVASEHGLEHVHTEVGVLGRNAAVRIFID
                     KPGGVTHADCSTVSHHVGAILEVEDWVNSAYTLEVSSPGLERGLYKREDYERFAGRLA
                     KMKSREAINGQRNFKGRIIGIEGDRVVFDDNTNGQVKVPVASIVKANLEIDVEEEFRI
                     AAERERAAKQNGGS"
     gene            complement(2508..2954)
                     /locus_tag="VGB76_11495"
     CDS             complement(2508..2954)
                     /locus_tag="VGB76_11495"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY0099564.1"
                     /translation="MSLKQRIIDDMTAAMRAQDAPRLSTLRMVKAAMMNRQIEKGGEL
                     TDEELTKMLGSLLKQRRDSVEQYERGGRAELAAKERAEIGVIEAYLPQAATREEIEEA
                     VRAAIAETGASSVKEMGAVMKAAQVRLAGRATDGRALSELVKAKLG"
     gene            complement(3079..6006)
                     /gene="uvrA"
                     /locus_tag="VGB76_11500"
     CDS             complement(3079..6006)
                     /gene="uvrA"
                     /locus_tag="VGB76_11500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0099565.1"
                     /translation="MTEEAITVRGARVNNLKNVDFTIPIERLTVVTGVSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLERMDKPDVDEVRGIPPAIAIRQKNSTRNPRSTVGT
                     QTEVYDYLRLLYARVGTTICRVCGREVHKDSPDSAAQETLGALAEGTRFYVLFPAEAG
                     LRLESASEQNGAGKKSGKARAKKGKAGADASALSRQMVTAHVMSLMQRGFTRLYANGE
                     TLELRSPDDYTRADFKDVYVLVDRLAARADVRSRLTDSLQTCFVEGHGAAIVQTAESE
                     PRTLKFSERFECRYDGTVYAQPEPRLFSFNNPFGACPTCQGFGNTIGLDLDLVIPNPL
                     LSIDEGAIEPWTKPQHEWAQTELRRFCRSEGIPTSVPYSELPRADQRAIVEGKGHWGG
                     VHGFFAWLETKKYKLHVRVFLAKYRGYTRCPECNGGRLRQEARDVRVGGRTLPDICKL
                     PVKETAVFFETLTLSDEQAAIADKVLFEVRRRLKFLVDVGLDYLTLDRLASTLSGGEA
                     QRIQLATNLGSALVGALYVLDEPSIGLHPRDSERLIKLLHNLRDIGNTVLVVEHDPDM
                     MRAADHILDVGPAAGELGGRIVFEGDFAGLLKDSATLTGKYLRGEAEIKEPKQRRTAK
                     KKRLSVRGASEHNLRGVDVDIPLEMFVCITGVSGSGKSTLVHDVLFAGLKKARGEWQG
                     HVGAFQKLEGGGQIDDVVLVDQSPIGRTPRSNPVTYIKVYDGIREVFAATRDAQAKGF
                     DASYFSFNVPGGRCDVCEGDGTVTVEMQFLADVELVCEECKGTRFKSNVLEVRYRGKG
                     INDVLSMTVREALTFFRDVNKIANKLRVLDEVGLGYLRLGQSATTLSGGEAQRIKLAS
                     FLAKRTGAKTLYIFDEPTTGLHFDDINKLLAAFRTLVEAGGSLLVIEHNLDVIKTADY
                     IIDLGPEGGDAGGQVVATGTPEEIMKVKNSHTGRFLKEHLARSNGHAKNGQPVAADDQ
                     TQS"
BASE COUNT         1090 a         1971 c         1840 g         1343 t
ORIGIN      
        1 caccagttcc tccaccgaca tctcttcaac ttccgaagcc gctacgggat cggcagattc
       61 ggcggccaga atttccgatt cggcgcgaaa cggcgtgccg ccgcgcacgg cctcgtcgta
      121 gccttcggcg atcatcgcgt ccgaaggttc gacttcgccc ggatcaatcg ttggatcgag
      181 ttcggcggag acggcgtgct gttccaacgc atcgtccggc gtcgcatcat ccaccgcgtc
      241 ggccagttct ccggcggcgg cttcggcttc cgccgtattc gcgccctctc catcgtcctg
      301 cttgctgcgc tcgcgcgcgg cgacgacgcg ttgcgccgaa tcgagaatgg cctgcgcttc
      361 gtcgagcgag atgtcgagat actccgtcag gtcgtcgagc gacgtaccgg ccagcgtgtc
      421 tatgtcttcg atgccgcgct cggcgagtgc ggcggtggtg gccgcgttca cgccttcgag
      481 cgcggtcagc ggaactgcgc cgccggaagc gatgagcgcg cccatctgct cggccacttc
      541 tttcttgagt tcctcttcgc tgcggatgtc tatgttccag ccgacgagtt tggtggcgag
      601 gcgcacgttc tgcccgcgct tgccaatggc taagctgagt tggtcttcgt tgacgatcac
      661 ttccatctgc ctgcgctcga tgtccgtgat gcgcacctga ttgaccttcg cgggcgacaa
      721 ggcgttcgcc gcaaacaccg aaggctcatc cgaccattcg atgatgtcga tcttctcgcc
      781 gcgcagttcc ctgatgatcg cctgcacgcg cgatcccttc atgccgacgc acgcgcccac
      841 cggatcaacg tcgcgctcgt tcgatgcgac cgcgatcttg gcgcgctctc cgggttcgcg
      901 cacagccgat ttgatgatca ccgtctcgtc gtaaatctcc ggcacttcca tctcgaacag
      961 gcggcgcagc agttccggcg aggtgcgtga gacttccact tgcggcccct tcgcttcctt
     1021 cgccacgtcg ttgatcacga cgcgaatgcg ctcgccctgt ccccactgtt cgccgcgcga
     1081 ctgctctttg cgatagagga tcgcttcgac cgtgccgaga tcgacgatga tgtcgccgcg
     1141 ctcgaagcgt ttgacgtagc cgttgacgag gtcgccgacc ttgtctatgt actgctcgta
     1201 aatgttctgc cgctcggcgt ccctgacctt ctgaaaaagg atctgcttgg cggtctgcgc
     1261 ggcgatgcgt cccatgtcct ccatcgggag gggcagttgg agttcgtcgc ccacgtccac
     1321 ctcgtcgccc gccatctctc tggcctcttc gagcgacagt tccttgccgg ggttcgtcac
     1381 ctcatccacg accactttcg aggcgaacag ttcgaaaccg ttctccgacg accactcgac
     1441 ctgaatgtct tcgcccgatt taaactgctt ccgcgcggcg gcacgcacgg cctccttcat
     1501 cgcctcgatg acgatctcac ggtctacgcc cttttccttg cacagggcgt caatactttg
     1561 cgcgatggat gcactcataa tttttttaga ggtgagaggc tggagattag aggttagtga
     1621 aagacagttt tcggtttaac tgaatctcta aacgctgaac ttcggcctct gctgtttatt
     1681 caattgtact aactgccgcc gttctgttta gcggcgcgct cgcgttctgc cgcgatgcgg
     1741 aactcttctt ccacgtcgat ctcaaggttc gctttaacga tgctcgccac aggcacttta
     1801 acctgcccgt tcgtgttgtc gtcgaaaacc acccggtcgc cttcgatgcc gatgatgcgg
     1861 cctttgaagt tgcgctggcc gttgatggct tcgcgcgatt tcatcttggc aaggcgtcct
     1921 gcgaaacgtt cgtagtcttc gcgcttgtag aggccgcgtt cgagtccggg cgaggacact
     1981 tcaagggtat aggctgaatt tacccaatct tccacctcaa ggatcgcgcc gacgtgatgg
     2041 ctgaccgtcg aacaatcggc gtgggtcacg cctcccggct tgtctataaa gatgcgcacc
     2101 gccgcgtttc gcccgagcac gccaacttcc gtgtgaacgt gttcaagccc gtgttcgctc
     2161 gccacgcgtt cgacaatttc gcgtatccgt ccttcaattt ccgacgcttc catcgctctc
     2221 gttcccagcc ccctttgttc agagatgccc cgcgcaaaat aacctgtgcc caaaataaaa
     2281 agtgggcgcg aacccactgg acaacgccac cgcgctccga gcaagagacg gattataaag
     2341 ggaaacgcgc aaaagggcaa aaccggaacg aagcaaaagc gggctggagg gcaacgggca
     2401 aaggctcgcg ggaaaagcgt gcgattttcg gccaaacttc gccgttctta acaaagagcg
     2461 gatttgaagg tttttccttt accccgttca gctttcccct ctaacgttta ccccaacttc
     2521 gccttgacga gttcgctgag cgcgcgcccg tccgtggcgc gacccgcgag tcgcacctgc
     2581 gccgccttca tcactgcgcc catctccttc acagagcttg cgcccgtctc cgcgatggcg
     2641 gcgcggacgg cctcttcgat ctcttcccgc gtcgccgcct gcggcagata cgcttcgatg
     2701 acgccgatct ccgcgcgctc tttcgccgcc aactccgcgc gcccgccccg ctcatattgc
     2761 tcgaccgaat ccctgcgttg tttgagcagc gacccgagca tcttcgtcaa ctcttcgtcg
     2821 gtcaactcgc cgcccttctc gatctgccgg ttcatcatgg cggctttcac catgcgcagc
     2881 gtcgagagtc gcggcgcatc ttgcgcacgc atcgcggcgg tcatatcgtc aataattcgt
     2941 tgtttgagac tcataaattt agcctttaac gattaacccg ccagcttttc gccagagcaa
     3001 tctccgtccg ctgtaggttc agaatttaga tgcaaaccaa cttcaatagg taaacttcaa
     3061 ccggcattta tctgatgatc agctttgcgt ctgatcgtcg gctgctacgg gctgaccgtt
     3121 tttcgcgtgc ccgttcgagc gcgcgaggtg ctctttgagg aaacgacccg tgtgcgagtt
     3181 tttaactttc atgatctctt cgggcgtgcc ggtggcgacc acttgcccgc ccgcgtcgcc
     3241 gccttcgggg ccgagatcga tgatgtagtc ggcggtcttg atcacgtcga ggttgtgttc
     3301 gatgacgagg agcgagccgc ccgcttcgac aagggtgcgg aaggccgcga gcagtttgtt
     3361 gatgtcgtcg aagtggaggc cggtggtcgg ctcgtcaaaa atgtagagcg tctttgcgcc
     3421 cgtgcgtttg gcgaggaagg aggcgagctt gatgcgctgc gcctcgccgc ccgaaagcgt
     3481 ggtcgccgat tggccgagcc gcaggtagcc taagcccact tcgtcgagca cgcgcagctt
     3541 gttggcgatc ttgttcacgt cgcggaagaa ggtgagcgct tcgcgcaccg tcatgctgag
     3601 cacgtcgttg atgcccttgc cgcgataacg cacttcgagc acgttcgatt tgaagcgcgt
     3661 ccccttgcac tcttcgcaga cgagttccac gtcggcgaga aactgcatct cgaccgtcac
     3721 cgtgccgtcg ccctcgcaca cgtcgcaacg cccgcccggc acgttgaagg aaaaatagga
     3781 ggcatcgaaa cctttcgcct gcgcgtcgcg cgtcgcggcg aagacttcgc ggatgccgtc
     3841 gtagactttg atgtaggtga ccggattcga gcgcggcgtg cgcccgatgg gcgactggtc
     3901 aacgagcacc acgtcgtcga tctgccctcc gccttccagt ttttgaaacg cgccgacgtg
     3961 tccctgccat tcgccgcgcg ccttcttcaa ccccgcgaac agcacatcat ggacgagcgt
     4021 tgacttgccc gaaccggaga cgcccgtgat gcagacgaac atctccaagg gaatgtccac
     4081 gtccacgccc cgcaaattat gctcgctcgc gccgcgcacg gagagcctct tcttctttgc
     4141 cgtgcgccgc tgcttcggct ctttgatctc ggcctcgccg cgcaggtact tgcccgtcag
     4201 cgtcgcggaa tccttcaaca gccctgcgaa gtcgccttcg aagacgatgc gcccgccgag
     4261 ttcgcccgcc gcaggtccaa cgtcgaggat gtgatcggcg gcgcgcatca tgtccgggtc
     4321 gtgttcgacg acgaggactg tgttgccgat gtcgcgcaaa ttatggagca acttgatcag
     4381 acgttcgctg tcgcgcgggt ggagtccgat tgacggctca tcgagcacgt agagcgcgcc
     4441 gacgagtgcc gagccgagat tggtggcaag ttggatgcgt tgcgcctcgc cgcccgagag
     4501 ggtcgaggcc agacggtcga gcgtcaaata atcgaggccg acatctacca gaaacttcaa
     4561 acggcggcgc acttcaaaga gcaccttgtc ggcgatggcc gcctgctcgt ccgagagcgt
     4621 gagcgtctcg aaaaatacgg cagtctcttt caccggcagc ttgcagatgt cggggagcgt
     4681 ccgcccgccg acgcgcacgt cgcgcgcctc ttgcctgagc ctgccgccgt tgcattcggg
     4741 gcagcgcgtg tagccgcgat acttggcgag aaagacgcgc acatggagtt tatatttctt
     4801 cgtctccagc cacgcgaaga aaccgtgtac gccgccccag tgtcctttgc cttcgacgat
     4861 ggcgcgttgg tcggcgcgcg ggagttcgct gtaaggaacg ctggtcggga tgccttcgga
     4921 gcggcagaag cgtctgagtt ccgtctgcgc ccattcgtgc tgcggcttcg tccagggttc
     4981 aatcgcgcct tcgtcgatgc tgagcagggg gttcgggatg acgagatcga gatcgaggcc
     5041 gatggtgttg ccgaagccct gacaggtggg gcacgcgccg aacggattgt tgaaactgaa
     5101 caggcgcggc tccggttggg catagaccgt gccgtcgtaa cgacactcga aacgttcgga
     5161 gaatttcagc gtgcgcggct cgctctccgc cgtctggacg atagccgcgc cgtgcccttc
     5221 gacgaagcag gtctggagcg aatcggtgag acggctgcgc acgtctgcgc gcgcggcgag
     5281 acgatccacg agcacgtaga catccttgaa atcggcgcgc gtatagtcgt cgggcgaacg
     5341 cagttcgagc gtctcgccgt ttgcgtagag acgcgtgaac ccgcgctgca tcaagctcat
     5401 cacatgcgcc gtcaccatct ggcgactgag cgcggaagcg tccgcgcccg ccttgccttt
     5461 cttcgcacgc gccttgccgc tcttcttgcc cgcgccgttc tgctcgctcg cactttctaa
     5521 gcgcagaccc gcttcggcgg ggaagaggac ataaaagcgc gtgccttcgg cgagcgcgcc
     5581 gagcgtctcc tgtgcggcgg aatcgggcga atccttgtgc acttcacgcc cgcagacacg
     5641 gcagatggtt gtgccgacgc gcgcgtagag gaggcggagg tagtcgtaaa cttcggtctg
     5701 cgtgccgacg gtggagcgcg ggttgcgggt cgagttcttc tgccggatgg cgatggcggg
     5761 cgggatgccg cgcacctcgt ccacgtccgg cttgtccatg cgttcgagaa actggcgcgc
     5821 gtaggacgac agcgactcga cataacgacg ctgcccctcg gcgtagatcg tgtcgaaggc
     5881 gagcgaagat tttcccgaac ccgacacgcc cgtcacgacc gtcagacgct cgatgggaat
     5941 agtgaagtca acgttcttta aattattgac gcgcgcgccg cgcactgtga tggcttcttc
     6001 agtcatgttt cacttcgacg ccggggcgca cagtcgggag tctggcgcac tagcgaaaca
     6061 cacatgataa cccgttttag cgcgaggacg aaacggcgaa gggcaggccg caggagagcc
     6121 cgcgccgcag acgcaaagac gcaaaggaaa gcgacggaag gattgaagtt ctcaatcgct
     6181 tactgtcgtt tgtcgccgcg cttccggcgt gaaatttctt ttagttgcag aagacgctca
     6241 ccag
//
LOCUS       DASWNS010000328        12072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_298019, whole genome shotgun sequence.
ACCESSION   DASWNS010000328 DASWNS010000000
VERSION     DASWNS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12072
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298019"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..772)
                     /locus_tag="VGB76_11505"
     CDS             complement(23..772)
                     /locus_tag="VGB76_11505"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0099566.1"
                     /translation="MFKKLFITATLALSFTIPVVAQNTNSSPAPQATPAVRRPSARRP
                     APLPTPTPVTLEAPETGQPTTDTLAPASRTRRTGATQATPATTATGATPAERAVRATF
                     ETLLNGIRKSDVEMVMSVYWNSPQLVIFNNNGTVTRSWEQVRSNRASAYPEAKNVQLE
                     VRDVRVSMLGAGGAVVSCLWTQSQEFRGTPESATGRLTVIFRLINNGWKIVHTHSSPS
                     APDPSRLLPSEQPPASTPPGAARPPAQEIRP"
     gene            complement(853..2340)
                     /gene="gltX"
                     /locus_tag="VGB76_11510"
     CDS             complement(853..2340)
                     /gene="gltX"
                     /locus_tag="VGB76_11510"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY0099567.1"
                     /translation="MRVRFAPSPTGYLHIGAARTALFNWLYARKLGGTFLLRIEDTDL
                     QRSTDESTRSIIEGLHWLGLDYDEEIVFQSANADAHRADALQLIVAGKAYRDFTPKAE
                     RADANVKQEIAERARAASSTGTDHRSNPFRDLSPEESEARAGAGEPFAVRLKVAREGR
                     SSFTDAVYGSQERDYSEIEDLVLLRSDGHPLYNLSVVCDDIEMRITDVIRGQDHLTNT
                     HKQILIYEALGAPVPRFAHLPLILAPNKAKLSKRKHGEVVSLTTYRDRGFVPAAFRNF
                     LALLGWSPNDEQELLSREQLVEKFTLEGIHRAPAVFNFNESDARQWTDQKAMWMNAEY
                     IRTMPVAELAPMVRAELEATGLWKPEYETHERAWFERAIEIIRQRFHTLKDFSGQGRA
                     YFADEFEFEEAAVRKNLQKEPRLKELLPALADKIEQTEPFTLETAETTLRAFADEVGV
                     KAGLFINAARTALTGQSVGASMFEVFDLIGRERSARRLREAARLI"
     gene            complement(2546..2636)
                     /locus_tag="VGB76_11515"
     tRNA            complement(2546..2636)
                     /locus_tag="VGB76_11515"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2600..2602),aa:Ser,seq:gct)
     gene            complement(2820..3485)
                     /locus_tag="VGB76_11520"
     CDS             complement(2820..3485)
                     /locus_tag="VGB76_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099568.1"
                     /translation="MEKRFTYLSLAALASLALTGTFFYSTAFSGQSERGQCQQTCTRQ
                     YQECRSGTNANDAACRQAFDTCRAGCRDNTNGNTNTNTNTNTNTNTNTNTNTNTNTNT
                     NTNTNGNTNGNTNGNMNTNGNMNTNGNMNTNGNTNGNMNTNGNMNTNGNMNTNGNMNG
                     NTNGNTNGNTNGNDNRPGVRRGRNPNPGTPRGPVNRNTNANTNGNTNGNTNGNTNNSN
                     RLR"
     gene            3750..4616
                     /locus_tag="VGB76_11525"
     CDS             3750..4616
                     /locus_tag="VGB76_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0099569.1"
                     /translation="MSEVHLTRRQFLRGLTAAGLLGVSGAAAYASLFEITDYELTATD
                     IFIRELPASFENFRIVQLSDVHHSALVSLAEVRRVVELAQAARGDMVVLTGDYTTARR
                     SYIEPCAEALGKLEAPSGVWAVLGNHDHYTDGELTRRALVRHGIGVLDNAHTKLQRGT
                     DALQLAGIDDWSWGYADWTRALGGLDRTRPTLLLSHEPQVFDAPETAGISLILSGHTH
                     GGQVSLPFVGAPARFLEAFKYIRGRYERAGAQLYVSRGTGVVGLPIRFGARPEIAVLR
                     LRRAPDAATGGR"
     gene            4768..5391
                     /locus_tag="VGB76_11530"
     CDS             4768..5391
                     /locus_tag="VGB76_11530"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY0099570.1"
                     /translation="MNDQTNLLPLSDYALHAVVFGVVVASWFVFGLAFALFLRRKPSQ
                     GTERRRDSSSKLGFALQGASYAFVWAFMRQTFTHIAPLGQLPDIALGLVTIALATGSV
                     WFMLSAMQRLGKQWSLTARVLEGHNLITEGTYRWVRHPIYTGMFGLLVANGLAVSRPY
                     ALLVGIAIFWVGTVIRVRSEERLLRATFGAEYEEYARRVPAVLPGIY"
     gene            complement(5494..6036)
                     /locus_tag="VGB76_11535"
     CDS             complement(5494..6036)
                     /locus_tag="VGB76_11535"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY0099571.1"
                     /translation="MKTIFTRASLVIGLAAILTASAFAQTAPQQQGDTEDEGRRGRQG
                     RRGGKIGRMGEPGEHRGGGRGQGMARRALRRLNLSDAQRSQLRDIEARYSQSFKTQHQ
                     ELRQLMEVRRAGGTLTPEQKQRAQQLRTEARANGERMHAEILALLTAEQRDQLKQMRE
                     EGQERRKARREAYGTPDDDQ"
     gene            6232..8286
                     /gene="dxs"
                     /locus_tag="VGB76_11540"
     CDS             6232..8286
                     /gene="dxs"
                     /locus_tag="VGB76_11540"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY0099572.1"
                     /translation="MKTDDLEAGGSGWFPFPASPVRFPLRFVACNAGASRATMRDEMR
                     LIQHINSPADLRRLSLEQLPEVAAEIREYIIETMARVGGHSGASLGEVELAVALHYAF
                     DTPTDRLVWDVGHQAYAHKILTGRRDTLPTIKQYDGLSGFLRRDESEYDTFGAGHAST
                     SLSAALGMAIARDREGKSHHVAALIGDSSLAGGMAMEAINQAGHLKTRLVVLLNDNEM
                     SIAPAVGALTGYLNRLREAQGYHRFKDEVGDVLRAVPSFGERLHHAAKTVKDAIAAAV
                     LPGALVNELGFKYIGYVDGHDTLALVHALREAQQITDSPVIVHALTTKGKGFPAREKN
                     YYAWHATGPFDPKTGTAIKSKAAAPTYTAVLGRTLCELMERDDKIVALTAAMPDGTGI
                     DMVLEKFPERSFDVGIAEQHCVTFAAGMSTEGLKPVCAIYSTFLQRGFDQLVHDVCLQ
                     DLNVKFCMDRAGIVGGDGPTHHGLLDIAYLRAVPNMVIMAPKDEGEMRDMLYTMMEHQ
                     GAAAMRYPRGNGVGADITRAPELLEIGKGEILRDEGGDIAILAYGSMVPASMQAAERL
                     ARDGIAATVVNARFVKPLDAGLLLALARTKRLILTVEEAYLAGGFGSAVMELLEENGL
                     LDGLRLVRMGVPDRIVTHGDAKLLLAKYGLDADGIYTRVKESIEVLEERRAGARPKSV
                     RS"
     gene            8638..9432
                     /locus_tag="VGB76_11545"
     CDS             8638..9432
                     /locus_tag="VGB76_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099573.1"
                     /translation="MEETQKIFEPTPVGRPTEGEETLVSGADGTLQTPRFDEVEAQVA
                     RPVVPLAGNFTKRGGVRRRQWPIALVLISALAGGVVSLLAFRLYQQRQQQSQVAEAQT
                     ETQTPETNAAANADSMPPVETATTQADAPVVFEEFDPAKEGVPATGVDSNGGGGEAKP
                     ADKPTEAEAKPPAAVREERKEEAKPAPRAAEPKPRLVETLTPPRQVEARGENVSRDER
                     RNDDEEYFEERRERRRERRNQRREERRGRNRNIDRIKDIFEGPPPA"
     gene            9615..10388
                     /locus_tag="VGB76_11550"
     CDS             9615..10388
                     /locus_tag="VGB76_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY0099574.1"
                     /translation="MSKRERIDKLLVERGLAASRTRAQALVMAGVVLVNEQRVEKASE
                     TFAPDASIRVRGADDPASRYVGRGGLKLEAALDAFGLDVRDFVCLDVGASTGGFTDCL
                     LQRGARRVVALDVGHNQLDWRLRQDARVEVREGVNARYLRREDFSTQFDLVVMDVSFI
                     SARKVLPALVPLLGGREEGRIILLIKPQFEVGRGEVGKGGIVTDAAQHARVVEEVNAA
                     ALDLGLEVCGVIDSPIQGADGNREFLALYKRAPTHAADA"
     gene            10407..11099
                     /locus_tag="VGB76_11555"
     CDS             10407..11099
                     /locus_tag="VGB76_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099575.1"
                     /translation="MKKLTEAFALARLQRDKAAYRSHLKVNYEPAARDCRACPTPGVC
                     CTDAHFVNVHITRLEPVAIRETLARTPRLDDGARRAVYARARLAVERYGLRASAASDT
                     YAQTYSCPLFAPGVGCLVHARAKPAPCIQHACYENWEEMPPVALQWRTERRVELLNEK
                     TYGAAWAWLPTPLWLTLVDPYGDGEELARLVRLWSARRSVGAAAAGASNAQHNAAHRV
                     ARERRSLPVIAR"
     gene            11250..11858
                     /locus_tag="VGB76_11560"
     CDS             11250..11858
                     /locus_tag="VGB76_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0099576.1"
                     /translation="MYKVEATGGMLQVIRTDMSATASSPELEISADAALVARVRAGDR
                     DAFGRLYDAYTRMVHGILLARVPYGEVDDLVQDVFLVALRKIDSLRDANAFGPWLAMI
                     TRNRAMDFHRRTRDTAELTDDLPAATSAPEAEATEVLNLIRLLPEAYREPLILRFVEG
                     MTGPEIAERTGLAPASVRVNIHRGMKLLRERLGETKSSVREH"
     gene            11863..>12072
                     /locus_tag="VGB76_11565"
     CDS             11863..>12072
                     /locus_tag="VGB76_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099577.1"
                     /translation="MSDYLWDKTGEPDADAEQLENLLGTLKYQPRPLEIPASAMPTAK
                     ARPATMIFSRPRLAIAASLLLMLLAG"
BASE COUNT         2133 a         3584 c         3967 g         2388 t
ORIGIN      
        1 tacttcaaac ggcgcgcatc cgttaaggcc ggatctcctg cgccggaggt ctggccgcgc
       61 ccggcggggt tgaggcaggc ggttgttcgg aaggcagcag gcgcgaaggg tctggcgcgg
      121 agggtgaact gtgcgtgtgg acgatcttcc aaccgttgtt gatgagacga aagatgacgg
      181 tgaggcgtcc ggtcgcgctc tcgggtgtgc cgcgaaattc ctgcgactgt gtccagaggc
      241 aggagacgac cgcgccgccc gcgccgagca tcgagacgcg cacgtcgcgc acttcgagtt
      301 ggacgttctt agcttccggg tatgcgctgg cgcgattgga gcgcacctgt tcccaactgc
      361 gcgtgaccgt gccgttgttg ttgaagatga cgagttgcgg cgaattccaa tagacgctca
      421 tcaccatctc gacatcggat ttgcggatgc cgttcaggag cgtctcaaac gtggcgcgga
      481 cggcgcgctc ggcaggtgtc gcgcccgtcg ccgttgttgc gggcgtggcc tgcgtcgcac
      541 ccgtgcggcg cgtgcgcgag gcgggagcca gcgtgtccgt cgtcggctgc cctgtctcag
      601 gtgcttccag cgtgacgggc gtgggcgtcg ggagcggcgc ggggcggcgc gcggaaggac
      661 gacgcacggc gggcgtcgcc tgcggcgcgg gcgaactgtt cgtgttctgc gcgacgaccg
      721 ggatggtgaa cgacaacgcg agcgtcgccg tgatgaataa ttttttgaac atgatgactc
      781 ctccgtaaaa ctgctcgaag gcgcggagga ggcatctcct cagacgcgcc ttcgaggtcg
      841 aacgttaaat tgttagatca agcgtgcggc ctcacgcaaa cgtcgcgcgg agcgttcgcg
      901 cccgatgagg tcgaagactt caaacatcga agcaccaacc gactgtcccg tcaaagccgt
      961 gcgcgccgcg ttgatgaaga gtccggcctt gactccgact tcgtctgcga aggcgcgcag
     1021 cgtcgtttcc gccgtctcca gagtgaaagg ctcggtctgc tcgatcttat ccgcgagcgc
     1081 gggcagcaac tctttgaggc gcggttcctt ctgcaaattc ttcctgaccg ccgcttcttc
     1141 aaactcaaac tcgtctgcga agtaggcgcg cccctggccg gagaaatctt tgagcgtgtg
     1201 aaaacgctgg cgaataattt cgatggcgcg ctcgaaccag gcgcgctcgt gcgtctcgta
     1261 ttccggcttc cagagtccgg tcgcttcgag ttcggcgcgc accatcggcg cgagttccgc
     1321 gacgggcatc gtgcggatgt actcggcgtt catccacatc gctttttgat ccgtccactg
     1381 gcgcgcgtcg gactcgttga aattgaagac ggcgggcgcg cgatggatac cttcgagcgt
     1441 aaacttttcc acgagttgct cgcgcgacaa gagctcctgt tcatcgttcg gcgaccagcc
     1501 gaggagcgcg aggaagttgc gaaaggcggc aggcacgaaa cctcgatcac gataggtcgt
     1561 taaggagacg acttcgccgt gcttccgttt tgagagcttc gctttgttcg gcgcgaggat
     1621 gagcgggagg tgcgcgaagc gcggcacggg cgcaccgagc gcttcgtaaa tgagaatctg
     1681 cttgtgcgtg ttcgtgagat gatcttggcc gcggataacg tcggtgatgc gcatctcgat
     1741 gtcgtcgcag acgaccgaca ggttgtagag cgggtggccg tcggagcgca gcaggacgag
     1801 gtcttcgatc tcggaatagt cgcgctcctg cgagccgtaa accgcgtcgg taaaactcga
     1861 acgaccttcg cgcgcaactt tcagacgcac ggcgaaaggc tcgcccgcac cggcgcgcgc
     1921 ctccgactcc tccggcgaga gatcgcgaaa ggggttgctg cgatgatccg tgcccgtcga
     1981 agaggcggcg cgggcgcgtt ccgcgatctc ctgtttgacg ttggcgtcgg cgcgttcggc
     2041 cttcggcgtg aagtcgcggt aagccttgcc cgctacgatc agttggagcg cgtcggcgcg
     2101 gtgcgcgtcg gcgttggccg actggaagac gatctcttcg tcgtagtcga ggccgagcca
     2161 gtgtagacct tcgatgatcg agcgggttga ttcgtcggtc gaacgctgga ggtctgtgtc
     2221 ttcgatgcgc aggaggaacg tgccgccgag cttgcgcgcg tagagccagt tgaagagcgc
     2281 ggtgcgcgcc gcgccgatgt gcaggtagcc ggtaggtgat ggtgcgaaac gaactctcat
     2341 agcacagtga cgagtgacga gtgacaagtg acgagaaaaa gccgtgaatc gtggatcgtg
     2401 aatcgattca atcagtcttc ttcgtattga cgattgacgt tctttctctt gtcgctaaaa
     2461 aaagtaacga gtgacacctg acgagtctgg gacaagtttg ccgccacttg tcactcgtca
     2521 cctgtcactc gtcactgcat cttaatggcg gagatagcag gattcgaacc tgcgatgggc
     2581 ttttgaccca taacggttta gcaaaccgcc gccttcagcc actcggccat atctccgcaa
     2641 aggctcgccg aaacaaggtt gatattctag cccgcgaagg ttgattgtca agcgcgcgcc
     2701 agaggcggcg catcgtgtgc agcggatttg aaggacgaaa gctgaagggc ggcattcaag
     2761 ctgatgaatg ccgcccgcag ttggccggtg ccggggagac gtgacgaaga gcgggctgtt
     2821 tacctcagcc tgttgctgtt gttggtgttg ccgttcgtgt tgccgttcgt gttgccgttg
     2881 gtattggcgt tggtgttcct gttgacgggg ccacgaggcg tgccggggtt cgggttgcga
     2941 ccgcgcctga cgccggggcg gttatcgttc ccgttcgtat tgccgttcgt attgccgttc
     3001 gtattgccgt tcatgttgcc gttcgtattc atgttcccat tggtattcat gttcccgttg
     3061 gtattcatgt tcccgttggt gttgccattg gtgttcatgt ttccgttggt gttcatgttg
     3121 ccattggtgt tcatgttccc attggtgttg ccgttcgtat taccgttggt gttcgtgttg
     3181 gtattcgtgt tggtgttggt attcgtgttg gtgttcgtgt tggtatttgt attggtgttc
     3241 gtgttggtgt tcgtgttgcc gttggtgttg tcacgacagc cggcgcggca cgtgtcgaag
     3301 gcttggcggc aggcggcgtc gttggcgttg gtgccgctac gacattcctg gtattgccgg
     3361 gtgcaggttt gctggcactg cccgcgctcg ctctgtccgc tgaaagccgt cgaataaaag
     3421 aaagtgccgg tcagcgccag tgaggcgaga gccgccagcg ataggtaagt aaatctcttc
     3481 tccacaggtg tttcctcctt gaaaacttgc ttacacgccc gtcaggagcg cgcttcgcac
     3541 gctggtgcga aaggtttgat cgggaaattc aggggctcgg ttagcctctt atttatcaca
     3601 tttatccagt cctgtttgta cgggggcgta ctgctgcaag cgtcggctga acttagccag
     3661 ccgtcctgga gccatccggc ctctccaacc cgcgccccgc aaacgcactg cggggtcgcc
     3721 cgctgcatgc aggtgctaca atccgggcga tgtctgaagt tcatctcacg cgcaggcaat
     3781 ttttgcgggg cttgacggcg gcggggctgc tcggcgtctc gggcgcggcg gcttacgcga
     3841 gcctgttcga gatcaccgat tacgagttga cggcgaccga tattttcata cgcgaactgc
     3901 ccgcgagctt cgagaacttt cgcatcgtgc agttgtcgga cgtgcatcac agcgcgctcg
     3961 tgtcgctcgc ggaagtcagg cgcgtggtgg aactggcgca ggcggcgcgc ggcgacatgg
     4021 tggtcttgac gggcgactac acgacggcgc ggcgcagcta catcgaaccg tgcgcggaag
     4081 ctttagggaa actcgaagcc ccttcgggcg tgtgggctgt gctcggcaat cacgatcatt
     4141 atacggacgg cgagttgacg cggcgcgcgc tcgtgcgcca cggcatcggc gtgctcgaca
     4201 acgcgcacac gaagttgcag cgcggcacgg acgcgctcca actcgcgggg atcgatgatt
     4261 ggtcttgggg ctacgcggat tggacgcgcg cgcttggcgg cctcgaccgc acgcgtccta
     4321 cgctgcttct ctcgcacgag ccgcaggtct tcgacgcgcc ggagacggcg ggcatctcgc
     4381 tcatcctgag cgggcacacg cacggcgggc aggtgagctt gccgttcgtc ggcgcgcccg
     4441 cgcgtttttt ggaagccttc aaatatatac gcgggcgtta cgagcgcgcg ggcgcgcaac
     4501 tctacgtctc gcgcggcacg ggcgtcgtcg gcttacccat ccgcttcggc gcgcgaccgg
     4561 agatcgccgt cctgcgtctg cgtcgcgcgc cggatgcggc gacgggcggc aggtaaccgg
     4621 gagcgcgtgt caaaaaaaaa ctttccggtc tgtctttgcg ctccggcaac tttgcgcgag
     4681 ttatcctctt tctaaaagat aagtctcagg caaaggcgca ggggaaaaga tggacagttg
     4741 tttcgacgtg cgcgcacggt ttttgccatg aacgatcaga cgaacctgtt gccgctttcg
     4801 gactacgcgt tgcacgccgt cgtgttcggc gtcgtcgtcg cgtcgtggtt cgttttcgga
     4861 ttggcgttcg cgcttttttt acgtcgcaag ccgtcgcagg gaacggagcg caggcgcgac
     4921 agcagttcga aactcggctt cgcgctgcaa ggcgcgagtt atgctttcgt gtgggcgttc
     4981 atgcggcaga cgttcacgca catcgctccg ctcggtcaac ttccggacat cgccctcggc
     5041 ctcgtgacaa tcgctctggc gacgggttcg gtgtggttca tgttgtctgc gatgcagcgg
     5101 ctcggcaagc agtggagttt gacggcgcgc gtgttggaag gtcacaactt gatcacggag
     5161 ggaacttacc gttgggtgcg acatccgatc tacacgggga tgttcgggct gctcgtcgcc
     5221 aacgggctgg cggtgagtcg cccgtacgca ttgctcgtgg gcatcgccat tttctgggtg
     5281 gggacggtca ttcgcgtccg cagtgaagag cgtctgctgc gtgcgacgtt cggcgcggaa
     5341 tatgaagagt atgcgcgccg cgttccggct gtcctgccgg gaatttattg agtgggaaaa
     5401 ggtaaaggag taaaggggaa gggggaagaa gaaacattca acctctcctc cctcttaccc
     5461 tttccccgta cccttttccc tcgcccttaa ttcttactgg tcgtcgtcgg gagtgccgta
     5521 ggcttcgcgt cgggctttgc gtcgctcctg cccttcttcg cgcatctgct tgagttgatc
     5581 gcgctgttcg gcggtgagca gtgcgaggat ttcggcgtgc atccgttcgc cgttggcgcg
     5641 cgcttcggtg cgtagttgtt gcgcgcgttg cttctgttcg ggcgtgagcg tcccacccgc
     5701 acgacgcact tccatcaact gtcgaagttc ctgatgctgc gtcttgaaac tctggctgta
     5761 gcgcgcttca atgtcgcgca gttggctgcg ctgcgcgtct gacagattca gacgccgcaa
     5821 cgcgcgacgc gccatcccct gcccgcgacc gccgccacga tgctcgcccg gctcgcccat
     5881 ccgacctatc ttgccgcccc tccggccttg acgaccgcga cggccttcat cttccgtgtc
     5941 gccctgctgc tgcggcgcgg tttgcgcgaa ggccgaggcc gtgagaatcg cggccagtcc
     6001 gatgacgagg ctcgcgcgtg tgaaaatagt cttcatccgt ttaactcctt cgaatccaaa
     6061 ttaaaattga cgattgcttg ctggcgtgcc gcgaggaatt tcgcacacac gcgccggtca
     6121 acccgcttgt tttcgagttg cctgcgttca ctcacgacgg tttagacgcg cccggtgcgc
     6181 cgccggtctg aaaaagagag aggaaaggat attaaaggga agggagaaag gttgaaaact
     6241 gatgatctcg aagctggcgg ttccggctgg tttccctttc ccgcttcccc tgtccgcttt
     6301 ccccttcgct tcgtggcgtg caacgcgggc gcgtctcgcg ctacaatgcg cgacgaaatg
     6361 agactgatcc agcacatcaa ctcgcccgcc gatttgcgtc ggctatcact tgagcagttg
     6421 ccggaggtgg ctgccgaaat tcgtgagtac atcatcgaga cgatggcgcg cgtcggcggc
     6481 cactcgggcg caagcctcgg cgaggtcgaa ctcgccgtcg cactccatta tgcgttcgac
     6541 acgccgaccg accgcctcgt ctgggatgtc ggccatcaag cctacgcgca caagatttta
     6601 accggacgac gcgacacgct gcccacaatc aaacagtacg acggcctgag cggtttcctg
     6661 cgccgcgacg aatcggagta cgacacgttc ggcgcggggc atgcttccac gtcgctctcg
     6721 gctgcgcttg ggatggcgat tgcccgcgac cgcgaaggca agtcgcatca cgtcgcagct
     6781 ttgatcgggg attcgagtct cgcgggcggc atggcgatgg aggcgatcaa tcaggcggga
     6841 catttgaaga cgcgactcgt cgtgctcttg aacgacaacg agatgtccat cgctccggcg
     6901 gtcggcgcgc tcacgggcta cctgaaccgt ctgcgcgagg cgcagggcta tcatcgtttc
     6961 aaagacgaag tgggcgacgt gctgcgcgcc gtgccgtcgt tcggcgaacg cctgcatcat
     7021 gcggccaaga ccgtcaagga tgccatcgcg gcagccgtct tgcccggcgc gctcgtcaac
     7081 gaactcggct tcaaatacat cggctacgtt gacggccacg acacgctcgc gctcgtccac
     7141 gcgctgcgcg aagcgcaaca gatcaccgat agccccgtca tcgtccacgc gctcacgacg
     7201 aagggcaaag gctttccggc gcgcgagaaa aattattacg cgtggcacgc gaccggaccc
     7261 ttcgacccga agacgggcac ggccatcaaa tcgaaggccg ccgcgccaac ctacacggct
     7321 gttctcggac ggacgctctg cgagttgatg gagcgcgatg acaagatcgt cgccctgacc
     7381 gccgccatgc ccgacggcac aggcatcgac atggtcttgg agaagtttcc cgaacgctct
     7441 ttcgacgtag gcatcgccga acaacactgc gtgacgtttg ccgccggaat gtccaccgaa
     7501 ggcttgaagc ccgtctgcgc gatctactcg acgttcttgc agcgcggttt cgatcaactc
     7561 gtacacgatg tctgtctaca ggacttgaac gtgaagttct gcatggatcg cgcaggcatc
     7621 gtcggcggcg acggcccgac gcatcacgga ctgctggaca tcgcctatct gcgcgccgtg
     7681 ccgaacatgg tgatcatggc tccgaaggac gagggcgaga tgcgcgacat gctctacacg
     7741 atgatggagc atcagggagc cgccgccatg cgctacccgc gcggcaacgg cgtgggggcg
     7801 gacatcacgc gtgcgccgga acttctggag atcggcaagg gcgaaatttt gcgcgacgaa
     7861 ggcggcgaca tcgccatcct cgcttacggt tcgatggtgc ctgcgtccat gcaggcggcg
     7921 gagcggctcg cccgcgacgg catcgcggcc acggtcgtca acgcgcgttt cgtcaaaccg
     7981 ctcgacgccg gactcctgct cgccttggcg cgcacgaagc gtctgatcct gacggtggag
     8041 gaagcctatc tcgcgggcgg cttcggttcg gcggtgatgg agttgctgga ggagaacggc
     8101 ctgctcgacg ggctgcgcct cgtgcgcatg ggcgtgcccg accgcatcgt gacgcacggc
     8161 gacgccaaac tcctgctcgc caaatacggc ctcgacgccg acggcatcta cacgcgcgtc
     8221 aaagagagca tagaggtgtt ggaggaacgg cgtgcgggcg cgcgcccgaa gtctgtgcga
     8281 agctgaaccg ggcgaaaaaa tagttacaca cacgacgagc gcgtcgcttc cctcaaaacg
     8341 ggagcggcgc gtttttcgtg ccgggagaca cgatttcgaa cacggggcgg aagtttccgt
     8401 ctcccgcaag cgaggcgcgc caggcattat gaaagtcatg aattcgcaat ttattgggga
     8461 cagtaacgac gtaaacaggg gcatctaagg agttggctgc atgatgggcg cgctccgcgc
     8521 gacgcgcggc agaggccgcg agaaagcggc ggggcgggtg gcctgcaatt tgcaattaag
     8581 tttccagacg aacagggtag agtagttttg taggacgctt gagggttggg agagtaaatg
     8641 gaagagacgc agaaaatttt tgaaccgacg cctgtaggca ggccgaccga aggcgaagag
     8701 acgctcgtga gcggcgcgga cggcacgctc cagacgccgc gcttcgacga agtggaggcg
     8761 caggtggcgc gccccgtcgt gccgctcgca ggtaatttca caaaacgcgg cggcgttcgt
     8821 cgtcgccagt ggccgattgc gctggtgttg atctcggctc tggcgggcgg cgtggtcagt
     8881 ctcctcgcgt ttcgcctcta ccagcagcgg caacagcaat cgcaggtcgc cgaggcgcag
     8941 accgagacgc agacgccgga gacgaacgcg gctgcaaacg ctgattcgat gccgcctgtc
     9001 gagacggcca cgacgcaggc cgacgctccc gtcgtgtttg aagagttcga cccggcgaag
     9061 gagggcgttc cggctaccgg cgtggatagc aatggtggtg gcggcgaggc caaaccggcg
     9121 gacaagccga cggaagcgga ggcgaagcct cctgcggcgg tcagggaaga gcgtaaagag
     9181 gaggcgaaac ccgcgccccg cgccgccgaa ccgaagccgc gtctggtcga gacgctgacc
     9241 ccgccgcgtc aggtcgaagc gcgcggcgag aacgtgagcc gcgacgagag acgcaacgac
     9301 gacgaagagt actttgaaga acggcgtgag cgtcgccgcg agcggcgcaa ccagcgacgt
     9361 gaagagcgtc gcgggcgcaa tcgcaacatt gatcgtatca aagacatctt cgaggggcct
     9421 ccgcccgctt aaagtctcat tcccgaatta acgcagccga gaggccggac gccgaacacg
     9481 tccggcctct ttttgtttgc gtgcgacagt cggtttgaca gggcgttgac aacgccgctc
     9541 ggcatccgcc gcgacggatg cctccgccgc tcgtgtgtcg taacggcgag cgggtttgcg
     9601 ttaaagtagc gcgcgtgtcg aaacgtgagc ggatagacaa attgctcgtg gagcgcggtc
     9661 tggccgcgtc gcgcacgcgt gcgcaggctc tcgtgatggc gggcgtcgtc ctcgtcaacg
     9721 agcagcgcgt ggagaaggct tcggagactt tcgcgccgga tgccagcata cgcgtgcgcg
     9781 gcgcggacga cccggcctcg cgctacgtcg ggcgcggcgg tttgaaactt gaggccgcgc
     9841 ttgatgcgtt cggtctggac gtgcgggatt tcgtctgcct cgacgtgggc gcttcgacgg
     9901 gcgggttcac cgattgcctc ttgcagcgcg gtgcgcggcg cgtggtcgcg ctcgatgtcg
     9961 ggcacaatca actcgattgg cgtctgcgac aggatgcgcg cgtcgaggtg cgcgaaggcg
    10021 tcaacgcgcg ctacctgcgg cgggaagatt tttccaccca attcgatctc gtcgtgatgg
    10081 acgtgtcgtt catctccgcg aggaaggtgc tgcccgcgct cgtgccgctt ctcggcgggc
    10141 gggaggaggg gcgcatcatc ctgctcatca agccgcagtt cgaggtgggg cgcggcgagg
    10201 tgggcaaggg cggcatcgtc acggatgcgg cgcagcatgc gcgcgtcgtt gaagaagtca
    10261 acgcggcggc gttagacttg ggactcgaag tgtgcggcgt gatcgattcg ccgattcagg
    10321 gcgcggacgg caaccgcgaa tttctcgcgc tctacaaacg cgcaccgacc cacgccgccg
    10381 acgcttgaga cgaatttgtg cctgacatga agaaactaac ggaagccttt gcgctcgcgc
    10441 gtttgcagcg cgacaaggcc gcttaccgga gccacctcaa ggtcaactac gaacccgcgg
    10501 cgcgagattg ccgcgcctgc ccgacgcccg gcgtctgctg caccgacgcg cacttcgtca
    10561 acgttcacat cacgcggctc gaacccgtcg ccatccgcga aactctggcg cgcacgccgc
    10621 gcctcgacga tggcgcgcgc cgtgccgtct acgcgcgtgc gcgcctcgcg gtcgaacgct
    10681 acggcctgcg cgcgtccgcc gcgagcgaca cttacgcgca gacctactcg tgcccgctct
    10741 tcgcgcccgg agtcggctgt ctcgtacacg cgcgcgcgaa gcccgcgccc tgcattcaac
    10801 atgcgtgcta tgagaactgg gaagagatgc cgcccgtcgc cttgcagtgg aggacggagc
    10861 ggcgcgtcga gttgttgaat gaaaagacct acggtgcggc gtgggcttgg ctgccgacgc
    10921 cgctctggct cacgcttgtt gacccgtacg gagacggcga ggaactcgcg cgcctcgtcc
    10981 gtctatggag cgcgcgccgc agcgtcggtg cggcggcagc gggagcgtcg aacgctcagc
    11041 acaacgccgc gcaccgcgtc gccagagagc gtcggagcct gcccgtcatt gcacgctgaa
    11101 ttgtcctccg gtgtaaaagt taaaaattct cgcaaccatc cctgttaacg cttgccgtcc
    11161 ctgtcgcgtc ttagaaaccg aacgcgcaaa cgctcttgta gatcgagctt gccggttgag
    11221 ttgaaagcgt ctagagaggt tcttcccgaa tgtataaggt agaggccaca ggcggcatgc
    11281 tacaggtgat cagaacggat atgtccgcga cagcctcatc ccccgaactt gagatttccg
    11341 ccgacgccgc actcgtcgca cgcgtgcgcg caggcgaccg cgacgccttc gggcggctct
    11401 acgacgctta cacgcgcatg gtacacggca ttctgctcgc gcgcgtgcct tacggcgaag
    11461 tggacgatct cgtgcaggac gtgtttctgg tcgcgctcag gaaaatcgat tcgctgcgcg
    11521 acgcaaacgc cttcggaccc tggctcgcca tgatcacgcg caaccgcgcg atggactttc
    11581 atcgacgcac gcgcgacacg gcggaactga cggacgacct gccagccgcg acgagtgcgc
    11641 ccgaagccga ggcgaccgaa gtgttgaact tgatccggct gctgccggaa gcctatcgag
    11701 agcctttgat cctgcgtttc gtcgaaggca tgacgggacc ggagatcgcg gagcgcacgg
    11761 gactcgcgcc cgcctccgtc cgggtcaaca ttcatcgcgg aatgaagttg ctgcgcgaac
    11821 gcttgggcga gacgaagtct tctgtgaggg agcattaaag ttatgagcga ttatctatgg
    11881 gacaagacgg gcgaaccgga cgcggacgcg gagcaactcg aaaacttgct cggcacgctc
    11941 aagtaccaac cgcgcccgct ggaaattccc gcgagcgcga tgccgacggc aaaagcgcgc
    12001 ccggcgacga tgatcttctc gcgcccgcgt ctcgccatcg ccgcctcgct cctcctgatg
    12061 ctgctcgcag gg
//
LOCUS       DASWNS010000329         4507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_64097, whole genome shotgun sequence.
ACCESSION   DASWNS010000329 DASWNS010000000
VERSION     DASWNS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64097"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2998
                     /locus_tag="VGB76_11570"
     CDS             <1..2998
                     /locus_tag="VGB76_11570"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0099578.1"
                     /translation="ATVTLVDAAGAEKTATTNDEGVYTFTNLAPGTYTVRATTTGFGI
                     FENTAVAVAAGRRGSLDIQLSVVLEREDVTVAADTPVSTEAENNQSALVIKGTDLDAL
                     PDDADELAAALQALAGPSAGPEGGQIFIDGFTGGRLPPKESIREIRINQNPFSAEFDR
                     LGFGRIEILTRPGTDKVRGQAFFNFGDESLNSRDPFSERRAPFQLRSYGGNLSGPIQK
                     RKSSFFLDFDRRETDANSIINAQFVEPTTFDLIRINRTLVVPRRNTSFSPRLDYQLNS
                     TNTLVARYRYSRTQSENQGVGDFSLDIDPFFGIDRTNSRESIGHNFQLTETAILSPTV
                     INETRFQFDRERSTSGGDISAPVINVRDAFTGGGSSAGTTQNTNTRYELQNYTSFTLG
                     LHSLKAGARLRSVSLSDFSEQGFNGSYVFAGQAPQRDAEGNIISPGISSIDQFRLFRQ
                     GVPGVLPTQLNVSGGNPLASVNQVDLGLFIQDDWRVRKDFTLSAGLRYETQSNISSNL
                     NFAPRLSFAYSPGAGARQPKTVFRGGFGIFYTRFGESLTLQADRFNGQNQQQFIISNP
                     GTDEEIVKRLSLLQLPQAQFEQRLAQELALAQTARNLLGQFSNLNDVPSVDQLSAFAI
                     PQTTRVVAPDLQAPYTIQSTASVERLLPGRVTMTVSYVNTRTLHLLRTRNINAPVAGV
                     RPLGNALGNVYQYESSGILNQNQLLVNTNTRFNPNFSIFANYTYGRANSNADGVGSFP
                     ADNYDLSNEYGRSALDVRHRFFLGGSISAPWGIRLNPLVFASTGSPFNITTGLDNNGD
                     TLFTDRPAFASAATLPENLRETPFGNFDIDPLPGTPLIPRNYGQNPGQFTVNLRINKS
                     FGFGDAPGAAGRRAAAASGQTPAGDQTAAAGGAAPAGGRRGGQGGAGGRGGARGGGAR
                     GGGAGGPFGAGGIFGAPGGGAEQKRYNIQLGINIANILNRTNPGPFTGNLSSRFFGLP
                     TQSGGFRGFGGGAGNRSVEAQIRFLF"
     gene            3202..3987
                     /locus_tag="VGB76_11575"
     CDS             3202..3987
                     /locus_tag="VGB76_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099579.1"
                     /translation="MKFTSPAVALALCLCVATASAQEKSSDKTKPASPAPTTNASAAT
                     PADKTSVVTAAMSPIELARVAYNAQGGEKFRNLKNMMLTGSVDLYAPNSTQSLPGRFG
                     MIVAGVKLRQDVQAPPLINFQLIFDGEQSYSSMRGMNLPPLTKFGLPVLARFDQPGYT
                     VTALPDKKKQRAFRIADAEGNSTDFYVEAATGRVVRFEIPYNQMTYSFEYKTFREVDG
                     VLVPVNFAQKLAAPQGDFIAEFKVKEVKLNQDLPADAFKIPEQ"
     gene            complement(4083..>4507)
                     /gene="frr"
                     /locus_tag="VGB76_11580"
     CDS             complement(4083..>4507)
                     /gene="frr"
                     /locus_tag="VGB76_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY0099580.1"
                     /translation="GTPTPLNQMASISVPDAQMLAVQPWDQSQLNAIEKAIRTADLGL
                     NPSNDGKMVRVPIPALTEERRRQLAKQVHEFAEEHRTAIRNIRRDANERLKKMLKDKT
                     ISEDNERDGLEEVQKLTNSYIGKVDELAKNKEQEILSV"
BASE COUNT          860 a         1505 c         1273 g          869 t
ORIGIN      
        1 cgcgaccgtc acgctcgttg acgcggcggg cgcggagaag acggcgacga cgaacgacga
       61 aggcgtctac accttcacaa atctcgcgcc gggcacttac accgtgcgcg cgacgacgac
      121 cgggttcggc atctttgaaa acacggcggt ggcagttgcc gccggacggc gcggatcgct
      181 cgacatccag ttgagcgtgg tcttggagag ggaagacgtg acggtggccg ccgacacgcc
      241 cgtcagcacc gaagccgaga acaatcaaag cgcgctcgtc atcaaaggca cagacctcga
      301 cgccttgccc gacgatgcgg acgaactggc cgccgcgctc caggctctcg ccggaccttc
      361 cgccggaccc gaaggcgggc agatcttcat cgacggcttt acgggcggtc ggctcccgcc
      421 aaaagagtcc atacgcgaaa ttcgcatcaa tcagaaccct ttctcggcag agttcgaccg
      481 gctcggattt gggcgcatcg agattctgac gcgccctggc acggacaaag tgcgcgggca
      541 ggctttcttc aacttcggcg acgagagcct caattcgcgc gacccgttct ccgagcggcg
      601 cgcccccttt caactgcgca gctacggcgg caacctgagc ggccccatcc agaaaaggaa
      661 gtcatccttc ttcctcgatt tcgaccgccg cgagacggac gccaactcca tcatcaacgc
      721 gcagtttgtc gaacccacga cgttcgatct gattcgcatc aatcgcacgc tcgtcgtgcc
      781 gcgccgtaac acgagtttca gcccgcgcct cgactaccag ttgaactcga ccaacacgct
      841 cgtcgcgcgc taccgctact cgcgcacgca gtcggagaat cagggcgtcg gcgacttttc
      901 gctcgacatc gatccgttct tcggcatcga ccgcaccaac agccgcgaga gcatcggcca
      961 taatttccaa ctcaccgaga cggccattct cagcccgacc gtgatcaatg agacgcgctt
     1021 tcagttcgac cgcgaaagaa gcacttcggg cggcgacatc tccgcgcccg tcatcaacgt
     1081 ccgcgatgcc ttcacgggcg gcggttcgtc agccggaacg acgcaaaaca ccaacacgcg
     1141 ttacgaattg cagaactaca cttcgttcac gctcggcctg cactcgctga aagccggtgc
     1201 gcgcctgcgc agcgtaagcc tctctgactt ctcggagcag ggcttcaacg gctcatacgt
     1261 cttcgccgga caagcgccgc agcgagacgc cgagggcaat atcatctcgc ccggcatcag
     1321 ttccatcgat caatttcgcc tcttccggca gggcgtgccg ggcgtcctgc ccacgcagtt
     1381 gaacgtgagc ggcggcaacc ctctggcgag cgtcaatcag gtagacctcg gcctgttcat
     1441 tcaggacgac tggcgcgtgc gcaaagattt caccttgagc gcgggcttgc gctacgaaac
     1501 gcagtcgaac atcagcagca atctcaactt cgcgccgcgc ctctccttcg cctattcgcc
     1561 cggcgcgggc gcgcgtcaac cgaagacggt ctttcgcggc ggcttcggca tcttctacac
     1621 gcgcttcggc gagagtctga cgttgcaggc cgaccgcttc aacgggcaga accagcagca
     1681 gttcatcatc tccaacccgg gcacggatga agagatcgtc aagcgtctgt ctctgctgca
     1741 actgccgcag gcacagttcg agcagcggtt ggcgcaggag ttggctctgg cacagacggc
     1801 gagaaatttg ctcggccagt tctcgaattt gaacgatgtg ccctccgtcg atcagttgag
     1861 cgcgtttgcg attccgcaga ccacgcgcgt cgtcgctccc gatctacagg ctccttacac
     1921 gattcaatcg acggcgagcg tcgagcgtct gctccccggt cgggtcacga tgacggtgag
     1981 ctacgtcaac acgcgcacgc tccacctgct gcgcacgcgc aacatcaacg cgccggttgc
     2041 aggcgtgcgc ccgctcggca atgcgctcgg caacgtctac cagtatgaat cgagcggcat
     2101 cctcaaccag aaccagttgc tcgtcaacac caacacgcgc ttcaatccga acttctcgat
     2161 cttcgccaac tacacctatg gccgggcgaa cagcaacgcc gacggcgtgg gaagttttcc
     2221 ggcggacaac tacgatctgt cgaacgagta cgggcgttcg gccttggacg tgcgccatcg
     2281 tttcttcctc ggcggctcga tctccgcgcc gtggggcatt cgcttaaacc cgctcgtctt
     2341 cgcttcgacc ggctcgccct tcaacatcac cacggggttg gacaacaacg gcgacacact
     2401 ctttacggat cgtcccgcgt tcgcgtccgc cgcgacgctc cccgaaaatt tgcgtgagac
     2461 gcccttcggc aacttcgaca tagaccctct gccgggcacg cctctcatcc cgcgcaacta
     2521 cggccagaat cccggccagt tcaccgtcaa cctgcgcatc aataagtcat tcggcttcgg
     2581 cgatgctccc ggcgctgccg gacggcgcgc agccgccgcg agcggacaga cgcctgccgg
     2641 tgatcagact gcggcagcgg gcggtgctgc tcctgccgga ggtcgtcgcg gcggtcaggg
     2701 aggggctggc gggcgcggcg gcgcaagggg cggcggcgca cgcggtggcg gcgcaggcgg
     2761 cccgtttggc gcgggcggca tcttcggcgc gcccggcggc ggtgcggagc aaaaacgcta
     2821 caacatccaa ctcggcatca acatcgccaa catcctcaac cgcacgaatc cgggaccctt
     2881 taccggcaat ctgagttcgc gctttttcgg actgcccacg cagtcgggcg gctttcgcgg
     2941 cttcggcggc ggggcgggca atcgcagcgt cgaagcgcag attcgcttcc tgttctaacg
     3001 cgaactcttc aaacggctga aacgtcgcca cataactcga aggcgcgaag gcgcaaagaa
     3061 gattcgtttc tctttctttg cgccttcgcg cctttcgtgg catactgccg tttcagtctc
     3121 gacgcaactt tccgcgtgat ttacttcagg caaactctca atccttcact taatcgctcc
     3181 gcccgacttt gaggtgtcca catgaaattc acatcccctg ccgtagccct cgcgctctgt
     3241 ctctgcgtcg ctacggcaag cgcgcaggaa aaatcttccg ataaaactaa accggcctcg
     3301 cccgcgccga ccacgaacgc ttccgctgca actcccgcgg ataaaacttc ggtcgtcacg
     3361 gcggcgatgt cgcccatcga actggcgcgc gtggcctaca acgcgcaggg cggcgagaag
     3421 tttcgcaacc tgaaaaacat gatgctcacc ggctcggttg acctctacgc gccaaactcc
     3481 acgcagtcgc tcccgggcag attcggcatg atcgtggcgg gcgtcaagtt gcgacaggac
     3541 gtgcaagccc cgcccctcat caacttccaa ctcatcttcg acggcgagca gagttattct
     3601 tcaatgcgcg gcatgaacct gccgccgctc acgaagttcg gcctgcccgt gctcgccagg
     3661 ttcgaccagc ccggctacac cgtcaccgcg cttccggaca aaaagaagca gcgcgcattt
     3721 cgcatcgcgg acgcggaagg caactcgacc gacttctacg tcgaggcggc gacggggcgc
     3781 gtcgtgcgct ttgagattcc ctacaatcag atgacctaca gcttcgagta taagaccttc
     3841 cgtgaagttg acggcgtgct cgtgcccgtg aacttcgcgc agaagttggc cgcgccgcag
     3901 ggcgacttta tcgccgagtt caaggtcaaa gaggtgaaac tcaatcaaga cctgcccgcc
     3961 gacgccttca agattcccga acagtaagtc ttcttgcggg gaagaggaaa aaggaaaagg
     4021 gtaagggaga aagcacgcgg cttctccctt acccttttta ccttttccct gttttagttt
     4081 acctagacgc ttaagatctc ctgctctttg ttcttcgcca gttcgtcaac tttgccgatg
     4141 tagctgttcg tcagtttctg cacctcttcc agcccgtcgc gttcgttgtc ttcggagatc
     4201 gtcttgtctt tgagcatctt cttcagacgc tcgttggcat cgcgccgtat gttgcggatg
     4261 gccgtgcggt gctcctctgc gaactcgtgt acctgtttag ccaattgcct gcgccgctcc
     4321 tcggtcaatg cggggatcgg cacgcgcacc atcttgccgt cgttcgacgg gttcagaccg
     4381 aggtcggccg tgcggatcgc cttctcaatc gcgttcagtt gcgactgatc ccacggctgc
     4441 acggccagca tttgcgcgtc gggcacgctg atcgaggcca tctgattgag cggcgtcggc
     4501 gtgccgt
//
LOCUS       DASWNS010000330        14215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_349971, whole genome shotgun sequence.
ACCESSION   DASWNS010000330 DASWNS010000000
VERSION     DASWNS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14215
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349971"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VGB76_11585"
     CDS             <1..427
                     /locus_tag="VGB76_11585"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0099581.1"
                     /translation="IFEPSHFGGGLPQVVAHTVVDGAPFCLGRNFQVTPLEVIHGRLP
                     VVAYRCNDFAYATDLSEIPPATLDGLRDLDVLVLDCLRLTAHPTHLSLERSLAYIEEL
                     KPRRAYLTHIAHDIKHARDSALLPVGVEFGYDGLEISDE"
     gene            512..1468
                     /locus_tag="VGB76_11590"
     CDS             512..1468
                     /locus_tag="VGB76_11590"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY0099582.1"
                     /translation="MKLFHGTDNAEIERPTVLTLGVFDGLHLGHQLIMQTVVERARAV
                     NAVPTVITFDPHPRAVLHPESAPPLLQTFDQKLEAFRMLGIEQAIVIRFDEQFAQLRA
                     EDFLRDVVRERLQAREVYLGRGFAFGRNREGNIELLKSASGRFGFHADEVAEVRLRGR
                     RISSSRIREMLAAGRVNLARRMLGRPYGVEGRVVRGDERGRTLGFPTANLQPQNRVIP
                     RGGVYVTATLIEGEWRRSVTNVGTRPTFAAREGEPSVETYVMDWNGDLYGDVVRVRFL
                     RRLRDERKFASVAELKAQIDRDVARTARYFTHRGVRNSLAII"
     gene            1691..3484
                     /locus_tag="VGB76_11595"
     CDS             1691..3484
                     /locus_tag="VGB76_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEY0099583.1"
                     /translation="MATTGKKSNSLIPREGNGQHENGGTHRQNGNGRATSALSLIPPA
                     PSPQQPATHDSVTHETVAAPAVEAQAKADEAARLASGWRVWWRTFQVTRVLGMLGFYL
                     FLENYEARSKFNLRMHARRLEEARTRGRAALLRAKVRASAGRAIDRLIRLVRYIVFRG
                     ADESSTKEARLNRQAVWLKTRLIELGPTFIKIGQSLGTRGDLLPLAYVKELSLLQDQV
                     PPFPTTEAYARIEAELGRTLQEAYAEIDAAPVAAASLGQVYRARLHTGEEVAVKVQRP
                     NLAATVAFDIAILRRLVARLARYPSFNENADWQGMLDEFDETIGEEMDYAQEARNAER
                     FRENFKDWRVIHVPRIHRTHSTARVLTMEFIRGTKVVEIEALRARRISPVKVNRLLIR
                     TYLKQLLEDGFFHADPHPGNLLVMDDGRLAFFDFGMTGRITPRLQAQMIDAFFHVVAR
                     DVHGLAQDLINLNFLKPGVDPELVRPVVEALFEHYLNLKLGDVNFKELTYDLAAVMYD
                     YPFRLPSNFTYIMRALMTLEGIGLVTDPNFSFFDTAKPYAKEFMLRREGKQFRKLILD
                     KFMGRESADGRIEWGKMWKLAKMAAKTYLEK"
     gene            complement(3560..5983)
                     /locus_tag="VGB76_11600"
     CDS             complement(3560..5983)
                     /locus_tag="VGB76_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HEY0099584.1"
                     /translation="MRLRLFIYTLASALLLAGALPSLLAAAQKSAARSQRKTALARPS
                     VFVADPRPPNVPAPEEVLGFRVGDDRKLASWASIVDYFKRLDAASERVKFEELGRTTL
                     GAPFVVATISAPENLARLSEFKEIQRQLADPRTIGGGGAGAERRARQLIARGKTIVLI
                     TCGIHSTEVGSTLSSMLIAHRLASSDDPLVRRILQDTIVLLVPSLNPDGVDIVKRWYD
                     RTLGTPYEGTSPPELYHKYVGHDNNRDWYAFTQVETQLTVDRLHNEWHPQIVHDIHQQ
                     GEYGARLFLPPYMQPVEPNVPPRIIEGYTELGNAMAAEMRAAGLTGITTNSTYDAWTP
                     ARAYSHYHGGVRILSETASAKLATPLNVEFKQLRTREGYDPQKESANFSPVWRGGEWH
                     LRDITNYMTTGAFALLRHAAENRERWLTRFYEIGREAVRERKNGELFAYVIPYSQSAS
                     QLQAMQKLIRILQRGGVEVDHAGAFTAGGVRYASGSAVIRMDQPYANFAKALLETQRY
                     PDLRGEGGQPISPYDVTAHTLSMLMGVEAKPISSPFKYPVLSARIYRDAAPVPRARAS
                     LALYRSHVPSMDEGWTRWVLEQNKIHFASLEDREVRAGNLRGKYDAILIPDHSPRALL
                     EGYRKGAMPEELTGGLGAEGVRALNEYVEAGGTLIFLNRASDFAINQLKLPVRNATEG
                     LKETEFYVPGSILRTELDTTHHVAAGMPRESIAWVEDSPVFELTDAKEAARVRVIARY
                     PAGANPLLSGWLLGAEKLKGKAALVEVTQGKGRIYLFGFRPQYRAQSLATYPLLFNAI
                     RGAQRELFD"
     gene            6316..8790
                     /locus_tag="VGB76_11605"
     CDS             6316..8790
                     /locus_tag="VGB76_11605"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEY0099585.1"
                     /translation="MSSGTIDVTRPAIVDEVSVPVAESYAVHKPFVPASTILPEFTAR
                     AVIVGAILGIIFGASSVYLALRVGLTVSASIPIAVMSISIFRALGRTSILENNISQTT
                     GSAGESIAAGIVFTMPALLILGYDLEIPRITWVALLGGWLGVLLMIPLRRALIVREHG
                     KLIYPEGTACAEVLVVGEKGGTDAKTVFAGFGVAALYKFLMSGTRLWNEAPAYTARGY
                     QGATVAADISPELLGVGFIIGPRVAAMMLGGGFLSALVLIPMVKFFGAGYTEPLFPVL
                     AGGPLISAMTSGQVQDLYIRYIAAGAVATGGIISLVRSLPTIIGAFKSSLGSFGGGKT
                     TLEGDKAVPRTERDMPMTFVLGGIGVLALAIFFWLNFQGSPGSGIGMNILSAILVIIF
                     GFFFATVSSRITGEIGSSSNPISGMTIATILITSLIFLVLGMGGVDSRVLALSVGAIV
                     CIAASNAGTTSQDLKTGYLVGGTPSRMQWGLMVGATTSALLIAWTLSYLNTSYTNLLP
                     SHYAGYRTTVNPGDEQWAKGDMKGGTLPGGPYYVHRLAEDQKGADGTVIPSGQYLVNQ
                     TGEVFYRLNPGVGGARRAIVTEGLPATPFVAGGNVTIGANTARGLDNNIYKVATVKEG
                     GATQTYYVDDAGMAKYKEIETGPNSKFDAPKAQLMAVLVDGVLTQKLPWALILIGAFI
                     SILLEIIGISALPFAVGMYLPFATSATIFIGGAVRALVERVSRGNRSLAEEESGPGVL
                     YSSGLIAGGAIAGLLLAIPQGLGKADLLNVGRFLPEALHESMIIGIIMFGVLTATLYY
                     IGRHGLSGKRSENLPSPPPPPTQLRS"
     gene            8935..9777
                     /locus_tag="VGB76_11610"
     CDS             8935..9777
                     /locus_tag="VGB76_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099586.1"
                     /translation="MSQSMPRRMRASSVLMLVPALLLVVAVTPGFGQTAGAQTSATPQ
                     TPTTANAQARTPIQTVTEFYKALRERRFRDAFALSIYKSALDGLSAEEFAELQPEFEK
                     LAGAVPEKIEFGGEQISGDTATVFARVGGENSQLEAITLMRGAGGAWIFGDAENEAIV
                     KRDGKEFFLKARIDTHHREVEALLLKLANAQAGYSVQHSGQYGDMAALLEGKPALRED
                     VESAKTLGYNFQITLGKDAKSYKINAEPIRYGRTGLLSYHMDQTGIQRKDTGGKPYHP
                     PVKK"
     gene            9893..10321
                     /locus_tag="VGB76_11615"
     CDS             9893..10321
                     /locus_tag="VGB76_11615"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0099587.1"
                     /translation="MPELMRSIVARMRIYVGNRRRARRYRMSVPFNVSLFDPKLNREQ
                     LEHAPYLEGTTSDLSASGLGLIVPAIRIDDRYLTAPDQTLHVLLEFPSGQIEMRVAPV
                     RYEQLDREGAPQGGYLIGVSILEMSDDARTRFEQYLKELT"
     gene            complement(10385..11038)
                     /locus_tag="VGB76_11620"
     CDS             complement(10385..11038)
                     /locus_tag="VGB76_11620"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEY0099588.1"
                     /translation="METTVEAGRARSAWSNLSVPELDHAEWARARPVHLTRYWSGEIA
                     PPERQGEARLLWDERALYVRFVCAQREPLIVNASPQRERKTIGLWDRDVCEIFVAPRA
                     DEVERYFEFEVAPTGEWLDLALHTTAEGRETEWDFSSGMKAAARVEPDSITLAMCMPW
                     EAFGVVEAPRAGERWRVNLFRCIGAGSTRGYLAWQPTHALEPNFHVPEKFGWLLFKD"
     gene            complement(11081..12418)
                     /locus_tag="VGB76_11625"
     CDS             complement(11081..12418)
                     /locus_tag="VGB76_11625"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0099589.1"
                     /translation="MGYLELLRQNRGFRQLWLGQVVSQLGDWFDTIAVMTLVLNLTGS
                     GRDVGLIMVARFLPSVVVGPLSGVVADRFNRRTVMLVSDILRAVVVLGFLFVRRPDQI
                     WLIYALTVLQLTFSTFFEPAKSAAIPSIVAERDLVTANAITSVTWSAMLTLGAVAGGL
                     VTGWFGTDAAFVVDSATYLLSAALIWRIRLPPRPPREKTRLTISKALGITDTLEGIRY
                     VRTRPRVLALLAVKPAWGLGGGILALLAVFGEKVFPVGQTAATGIGVLYAARGIGTAL
                     GPVFARRLAGESRTRMQTIIGLSFLVGGVFYAAFGGATNYALALLMLLIAHMGGSILW
                     VFSTVLLQTAIEDNFRGRVFAAELALLTLTMAASNYVTGELLDRFNYPPRAVTIGIGI
                     LFLLPGLLWFATARWWNREPARAPQTSTPEDSPSATSDAAIRDAATKIHLSTD"
     gene            13195..>14215
                     /locus_tag="VGB76_11630"
     CDS             13195..>14215
                     /locus_tag="VGB76_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0099590.1"
                     /translation="MIDMSDYSSAAKAYWLALVSAGALIAAWALSQCLSFTPVQGLQF
                     VALTSFVVLSSSLPIRIPNTSVSVTAGDAFIFLGTILLGVPAALLLGATDALVRSFGS
                     SRRATSWFAAPATMSLTVLVSASAFYFLLNSYAGVARQPVGMARLTFEQLLVPLVAMA
                     LLQYFFNGMLLATLHALKEQSTIWKFWRNGYLWTSCTFFAAAIASALLYLAMQNFGLI
                     YVLLSVPVIGATFATYKIYFERINERSRETEEMARLHLATVEALATAIDAKDQTTHCH
                     VRRVQLYAARMGEILHLSHSEIEALRAGAMLHDIGKLAVPDHILNKPGKLTTAEFERM
                     KVHTIV"
BASE COUNT         2658 a         4401 c         4418 g         2738 t
ORIGIN      
        1 catcttcgag ccgtcgcatt tcggcggcgg gctgccgcaa gtcgtcgccc acacggtggt
       61 ggacggcgcg ccgttctgtc tcggtcgaaa ttttcaggtg acgccgctcg aagtcataca
      121 cgggcggctg cccgtcgtcg cctatcgctg caacgacttt gcctacgcca cagacctcag
      181 cgagattccg cccgcgactc tagacggcct gcgcgacctc gacgtgctcg tgctcgattg
      241 cctgcgcctc accgcgcacc cgacgcacct ctcgctcgaa cgctcgctcg catacatcga
      301 agaactcaaa ccgcgccgcg cctacttgac ccacatcgcg cacgacatca aacacgcgcg
      361 cgactccgcc ctgctgcccg tcggcgtcga gttcggctat gacgggttag agataagtga
      421 tgagtgatgg atgatgagta atgagtcaaa acaacaaact cgtctttgat ttattacgca
      481 tcacgcatca ctcatcaccc atcacttttt tatgaaatta tttcacggaa ctgacaacgc
      541 ggagatcgag aggccgaccg tgttgacgct cggcgtgttc gacgggctcc atctcggcca
      601 ccagttgatc atgcagacgg tggttgagcg tgcgcgtgcg gtaaatgctg tgccgaccgt
      661 catcactttc gacccgcacc cgcgcgccgt gctccatccc gaatccgcgc cgcccctctt
      721 gcagaccttc gaccagaagc tcgaagcctt ccgcatgcta ggcatcgaac aggcgatagt
      781 catccgcttc gacgagcagt ttgcgcagct tcgcgcggag gattttttgc gcgacgtggt
      841 gcgcgagcgt ttgcaggcgc gggaggtgta tctcggtcgc ggcttcgcgt tcgggcggaa
      901 tcgtgaaggg aatatagagt tgttgaaatc cgcgagcggt cgcttcgggt ttcacgcgga
      961 cgaagtggcg gaggtgcgcc tgcgcgggcg gcggatcagt tcgagccgga ttcgcgagat
     1021 gctggcggcg gggcgcgtga accttgcgcg gcgcatgttg ggacggcctt atggcgtcga
     1081 agggcgcgtc gtgcggggcg acgagcgcgg gcgcacgctc ggcttcccga cggccaacct
     1141 gcaaccgcag aatcgcgtca tcccgcgcgg cggcgtctat gtgacggcca ctctgatcga
     1201 aggcgagtgg cggcgcagcg tcaccaacgt cggcacgcgc ccgactttcg cggcgcgcga
     1261 aggcgagcct tccgtcgaga cttacgtgat ggactggaac ggcgacctct acggcgacgt
     1321 ggtgcgcgtg cgtttcctcc ggcggctgcg cgacgagcga aagttcgctt ccgtcgcaga
     1381 gttgaaggcg cagattgacc gtgatgtcgc acgcaccgcg cgctatttca cacatcgcgg
     1441 cgtgcgaaat tcgctggcga ttatttgaag gcggcgcgac ccgttcaaac acgcgagcgc
     1501 gccgcgccgc gcaatcctct ctcccgcccg cacacacccg aaaaccgttc caacatccgc
     1561 cgcgcaggcg cgaaatacgc ggcgggcgaa acaattttaa gacgcatcta atctttgttc
     1621 aggaacgtac aaggaatgaa cctgactgtc acaggattgc gacaagtgtg atttatcatc
     1681 gaaagcgaac atggcaacga ccgggaaaaa atcgaactct ttaattccgc gcgaaggcaa
     1741 cgggcagcac gaaaacggcg gcacgcaccg gcagaacggc aacgggcgcg cgacttccgc
     1801 cctgagtctc attcctcccg cgccctcgcc gcaacagcca gcgactcatg acagcgttac
     1861 gcatgagacg gttgctgcgc ccgccgtcga agcacaagcg aaggcggatg aagcggcgcg
     1921 tttggcgagc ggctggcgcg tgtggtggcg cacatttcag gtgacgcgcg tgctcggcat
     1981 gctcggcttc tacctgtttc tggaaaacta cgaagcgcgt tcgaagttca acctgcggat
     2041 gcatgcgcgg cggctcgaag aggcgcgcac gcgtggccgc gcggctttgc ttcgcgcgaa
     2101 ggtgcgcgcc tctgccgggc gcgcgattga tcgtctgata cgcctcgtgc gttacatagt
     2161 ctttcgcggc gcggacgaat cgagcacgaa ggaagcgcgc cttaatcgtc aggccgtgtg
     2221 gctcaagaca cgcctgatcg aactcggccc cacgttcatc aagatcggcc aatcgctcgg
     2281 cacgcgcggc gacctgctgc cgctcgccta cgtcaaagaa ctttcgctcc tgcaagatca
     2341 agtgccgccg tttccgacga cggaggctta cgcgcgcatc gaagcggaac tcggtcgcac
     2401 gctgcaagag gcttacgcgg agattgacgc cgcgcccgtg gccgccgcct cgctcggtca
     2461 ggtctatcgc gcacgacttc atacgggcga agaggtcgcg gtcaaagtgc aacgccccaa
     2521 tctcgctgcg acggttgcgt tcgacatcgc catcctgcgc cgcctcgtcg cgcgtctggc
     2581 gcgctatcct tcgtttaacg agaacgccga ctggcagggc atgctcgacg agttcgacga
     2641 aaccatcggc gaagagatgg actacgcgca ggaagcgcgc aacgccgaac gcttccgcga
     2701 gaacttcaaa gactggcgcg tcattcacgt cccgcgcatc caccgcacgc attcaaccgc
     2761 tcgcgtcctg acgatggagt ttattcgcgg aacgaaggtg gtcgagatcg aggctttgcg
     2821 cgcgcggcgc atctcgcccg tcaaagtcaa tcgcctgttg attcgcacct acttgaagca
     2881 gttgctcgaa gacggtttct ttcacgccga cccgcatccc ggcaacctgc tcgtgatgga
     2941 cgacgggcgg ctcgccttct tcgatttcgg catgaccggg cgcatcacgc cgcgcctgca
     3001 agcgcagatg atcgatgcct tcttccacgt cgtcgcgcgc gacgtgcacg gcctcgcgca
     3061 ggatttgatc aatctcaact tcctcaaacc cggcgttgac cccgaactcg tgcgcccggt
     3121 cgtcgaagcg ctcttcgagc actatctgaa tctgaaactc ggcgatgtga acttcaaaga
     3181 gttgacctac gatctcgcag cagtgatgta cgactacccg ttccgcctgc cttcaaactt
     3241 cacctacatc atgcgcgcgc tgatgacgct tgaaggcatc ggcctcgtca ccgatccgaa
     3301 cttcagtttc ttcgatacgg cgaagcctta cgccaaagag tttatgctgc ggcgcgaggg
     3361 caagcagttt cgcaaactca tcctcgataa gttcatggga cgcgaaagcg cggacgggcg
     3421 catcgaatgg ggcaagatgt ggaaactcgc caagatggcc gccaagacct atctggaaaa
     3481 atagcaggca gcgagagaca gtttaggtaa gaccggggcg acgccgcgaa cgtgattgtt
     3541 tgcggcgtcg cttatagttt taatcgaaga gttcgcgctg cgctccgcga atcgcgttga
     3601 agaggagcgg gtaggtggcg agcgattggg cgcggtattg cgggcggaag ccgaagagat
     3661 agatgcgccc cttaccctgt gtgacttcga cgagcgcggc cttgcctttg agtttctccg
     3721 cgccgagcag ccagccggaa agaagcgggt tggcacccgc cggatagcgc gcgatgacgc
     3781 gcacgcgcgc ggcctctttc gcgtccgtca actcgaagac gggcgaatct tccacccacg
     3841 cgattgactc gcgcggcatc cctgcggcga cgtggtgcgt cgtgtcgagt tccgttcgca
     3901 ggatcgagcc gggcacgtag aactccgtct ctttcaatcc ttccgtcgca ttgcgcacgg
     3961 ggagtttgag ttgattgatg gcgaagtcgg aggcgcgatt gagaaagatg agcgtgccgc
     4021 ccgcttcgac gtattcgtta agcgcacgca cgccttccgc gccgaggccg cccgtcaact
     4081 cttcgggcat cgcgcccttg cgatagcctt cgaggagcgc gcgcggcgaa tgatccggga
     4141 tcaggatggc atcgtacttc ccgcgcaagt ttcccgcgcg aacttctcta tcttcgagag
     4201 aagcgaagtg aatcttgttc tgttcgagca cccagcgcgt ccaaccctcg tccatcgaag
     4261 ggacgtgcga gcgatagagc gcgaggctcg cccgcgcacg cggcacgggc gcggcgtctc
     4321 tgtagatgcg cgcgctgagc acaggatatt tgaacgggct tgagatgggc ttggcttcga
     4381 cgcccatcag catggagagc gtatgcgccg ttacgtcgta gggcgagatg ggctggccgc
     4441 cttcgccgcg caaatcagga taacgttgcg tctcaagcaa cgccttggca aagttggcat
     4501 aaggttgatc cattctgatc accgccgacc cgctcgcata cctgacgccg cccgccgtga
     4561 acgctcccgc gtgatcaact tcgacgccgc cccgctgcaa gatacgtatt aacttttgca
     4621 tcgcctgcaa ctgcgaggcc gattgtgaat acggaatgac gtaagcgaac agttcgccgt
     4681 tttttcgttc gcgcacggct tcacgcccga tctcgtagaa gcgcgtgagc catcgttcgc
     4741 ggttctctgc cgcatgacgc aggagcgcga atgcgcccgt cgtcatgtag ttcgtgatgt
     4801 cgcgcaggtg ccactcgccg ccgcgccaga cggggctgaa gttggcagac tctttctgcg
     4861 ggtcgtagcc ttcgcgcgtg cgcagttgtt tgaactcgac gttgaggggc gtggctaatt
     4921 tcgcggaggc ggtttcggag aggatgcgaa cgccgccgtg atagtgcgag taggcgcgcg
     4981 ccggagtcca tgcgtcgtag gtggaattgg tggtgatgcc ggtgagtccg gcggcgcgca
     5041 tctctgcggc catcgcgttg ccgagttccg tgtagccttc gatgatcctg ggcgggacgt
     5101 tcggctcgac gggctgcatg tagggcggca ggaaaagacg cgcgccgtat tcgccctgct
     5161 ggtgtatgtc gtggacgatc tgcgggtgcc attcgttgtg cagtctatcg accgtgagtt
     5221 gcgtctcaac ctgcgtgaag gcgtaccagt cgcggttgtt atcgtgcccg acgtacttgt
     5281 gataaagctc cggcgggctg gtgccttcgt agggcgtgcc gagcgtgcgg tcgtaccaac
     5341 gcttgacgat gtcaacgccg tccgggttca gggaagggac gagcaggacg atggtgtcct
     5401 gcaaaatgcg gcgcacgagc gggtcgtccg acgacgcgag gcggtgcgcg atgagcatgc
     5461 tggagagcgt cgaaccgact tccgtggagt gtatgccgca ggtgatgagg acgatggttt
     5521 tgccgcgcgc gatgagttgc cgcgctctgc gttcggctcc cgcgccgcct ccgccgatgg
     5581 tgcgcgggtc tgcgagttga cgctgaatct ctttgaactc cgagaggcgc gcgagatttt
     5641 ccggcgcgct gatggtcgcc acgacgaagg gtgcgccgag cgtcgtcctg cccaactctt
     5701 caaacttcac gcgctcgctc gccgcgtcga gtctcttaaa gtaatccacg atgctcgccc
     5761 aactcgccag cttccggtcg tcgcccacgc gaaagccgag cacctcttcg ggcgcgggca
     5821 cgttcggagg gcgcgggtcg gccacgaaaa ccgacgggcg cgcgagggcg gtttttctct
     5881 gcgagcgagc cgcgctcttt tgcgcggcgg ccagcaggga agggagcgcg cccgcgagca
     5941 gcaacgcgga cgcaagcgtg tagatgaaga gacgtagacg cataacttgg tcgcaactcc
     6001 ggcgattgaa gtgtctgtaa aaatctgtgt tgtagaacgt gcgcggggat tttagcataa
     6061 cgcgcggagg gctgtccgtg gccggtcgcc cgttgccgtt tgtttgtctc ggacgtgcga
     6121 gcggacacgg cggcgacaaa agaattagtt gaacgactga caacggacga ccgcaaacgg
     6181 acaactaacg actgaccgtc tcccttgact gtctcccttg cccttggcct gtgtgcgcgt
     6241 gtaaagtaac ggctgaaatt acccctgcac actccacgtt tagttaaatc attctttcgg
     6301 aggtcacaga ccgcaatgtc gagtggaacc attgatgtta cgaggccggc tattgttgac
     6361 gaggtgagcg tccccgttgc cgagagctat gcggtgcaca agcctttcgt gcccgcttcc
     6421 acgatcttgc cggaatttac ggcgcgcgcc gtgatcgtcg gggcgattct ggggatcatc
     6481 ttcggcgcgt cgagcgttta tctggcgctc cgcgtcggcc tcacggtgtc ggcttcgatc
     6541 ccgattgccg tgatgtccat ctcgatcttt cgtgctctcg ggcgcacttc gatcctcgaa
     6601 aacaacatct cgcagacgac cggctcggcg ggcgaatcca tcgccgccgg aatcgtcttc
     6661 acgatgcccg cgctcctgat actcggctac gatctggaaa tcccgcgcat cacctgggtg
     6721 gctctgctcg gcgggtggct cggcgtgctg ctgatgattc cactccggcg cgcgctcatc
     6781 gtgcgcgagc acgggaagct tatctacccg gaagggactg cgtgcgcaga ggtgctcgtc
     6841 gtcggcgaga agggcggcac ggatgccaag accgtctttg cgggcttcgg cgtcgccgcg
     6901 ctctacaagt ttctgatgag cggcacgcgc ctgtggaacg aagcgcccgc ttacacggcg
     6961 cgcggctatc agggcgcgac ggtcgcggca gacatctcgc cggaactcct cggcgtgggc
     7021 ttcatcatcg ggccgcgcgt ggcggcgatg atgctcggcg gcggctttct gtccgcgctg
     7081 gtgctcatcc cgatggtcaa gtttttcggc gcgggctaca cggagccgct ctttccggtg
     7141 ctcgcgggcg gcccgctcat ctccgcgatg acttcggggc aggtgcaaga cctctacata
     7201 cgctacatcg ccgcaggcgc ggttgcgacg ggcggcatca tcagcctcgt gcgctctctg
     7261 ccgacgatca tcggcgcgtt caagtcttcg ctcggcagct tcggcggcgg gaagacgacg
     7321 ctcgaaggcg acaaggccgt cccgcgcacg gaacgcgaca tgccgatgac tttcgtgctc
     7381 ggcggcatcg gcgtgctcgc gctcgcgatc ttcttctggc tcaactttca aggctcgccc
     7441 ggcagcggca tcggcatgaa tatcctgtcg gccatcctcg tcatcatctt cggcttcttc
     7501 ttcgccaccg tctcttcgcg catcacgggc gagatcggtt cttcgtcaaa cccgatttcg
     7561 ggcatgacca tcgcgacgat cctgatcacg tcgctcatct tcctcgtgct cggcatgggc
     7621 ggagttgact cgcgcgtgct ggctctgtcg gtcggcgcga ttgtctgtat cgcggcctcg
     7681 aacgcgggca cgacttcgca ggatttgaag acgggctacc tcgtcggcgg cacgccctcg
     7741 cgcatgcagt ggggcttgat ggtgggcgca accacctccg cgctcctcat cgcctggacg
     7801 ctctcttatc tcaacacctc ctacacgaat ttgttgccga gccactacgc gggctatcgc
     7861 acgaccgtca accccggcga cgagcaatgg gcgaagggcg acatgaaggg cggcacgctc
     7921 cccggtggtc cttactacgt gcatcgcctc gccgaagatc aaaagggcgc ggacggcaca
     7981 gtcatccctt caggccagta tctcgtcaat caaacgggcg aagtcttcta tcgcctcaac
     8041 cccggcgtcg gcggggcgcg gcgcgcgatt gtgaccgaag gattgccggc cacgcctttc
     8101 gtcgcgggcg gcaacgtcac catcggcgcg aatacggcgc ggggcttaga caacaacatc
     8161 tacaaagtgg cgaccgtcaa agaaggcggc gcgacgcaaa cttattacgt ggacgacgca
     8221 gggatggcga agtacaagga gatcgagacg ggacccaact cgaagttcga cgcgccgaaa
     8281 gcgcagttga tggccgtgct cgtagacggc gtgttgacgc agaaactccc gtgggctttg
     8341 attctcatcg gggcgttcat ctcgatcctg cttgagatca taggcatctc cgcgctgccg
     8401 ttcgcggtcg gcatgtatct gccgttcgcc acgtcggcca cgatcttcat cggcggcgcg
     8461 gtgcgcgcgc tcgtcgaacg cgtctcgcgc ggcaatcgtt cgctggcgga agaggagagc
     8521 gggccgggcg tgctctacag ttccggcttg atcgcgggcg gcgcgattgc gggtctgctg
     8581 ctggcgatcc cgcagggact cggcaaggcc gacctgctta acgtcggtcg cttcctgccc
     8641 gaagccttgc acgagagcat gattattggc atcatcatgt tcggcgtgtt gaccgcgacg
     8701 ctctattaca tcggacgcca cggtctgtca ggtaaacgca gcgaaaatct cccctcgccg
     8761 ccgccaccgc cgacgcaact acggagttga ccgttcggct gacagcgttt ttgacaactt
     8821 gctggcaacg tattagttgt cccgttcctc tataatgtgc ggcgcgacgt gcgcgattct
     8881 ctagtcgccc ggacgcgccg cacacatctc atctgtccga aggaaatacc cgacatgtca
     8941 cagtccatgc ctcgccgcat gcgcgcgtca tccgtcctaa tgctcgtccc ggccttgctg
     9001 ctcgtcgtcg ccgtcacgcc cggtttcggc cagaccgcag gcgcgcaaac ttcagccacc
     9061 ccgcaaactc cgacgaccgc caatgcgcag gcgcgcacgc cgatccagac cgtgaccgaa
     9121 ttttacaagg ctctgcgcga gcgacgtttt cgcgacgcct tcgcgctctc gatctacaag
     9181 tccgcgctcg acggcttgag cgcggaggag tttgccgagt tgcaaccgga gtttgaaaaa
     9241 ctggccggag ccgtgccgga gaagatcgag tttggcggcg aacagatcag cggcgacacg
     9301 gcgaccgtct ttgcgcgcgt cggcggggag aactcgcagt tggaagcgat cacgttgatg
     9361 cgcggggcgg gcggcgcgtg gattttcggc gacgcggaga acgaggccat cgtcaaacgc
     9421 gacggcaagg agttctttct caaggcgcgc atcgatacgc accacagaga agtcgaagcg
     9481 ttgctcttaa agctggcgaa cgcgcaggcg ggctacagcg tgcagcacag cgggcagtac
     9541 ggcgacatgg ccgcgctcct cgaaggcaaa ccggccttgc gcgaagatgt cgagagcgcg
     9601 aagacgctcg gctacaattt tcagatcacg ctcggcaagg acgcgaagag ttataagatc
     9661 aatgccgaac ccattcgcta cgggcgcacg ggtctgctct cctaccacat ggatcaaacg
     9721 ggcatccaga ggaaggacac gggcggcaag ccctaccacc cgccggtcaa aaaataggcc
     9781 agatggtaga gggagagggg tagacgggaa gggaaaggaa aagatcgaac cgccgcgctt
     9841 ctgctttacc ctttgacctt tcccccttcc ccttttcccc tgcttaaacc ttatgccgga
     9901 actgatgcgt tccatcgtcg cgcgcatgcg catttatgtc ggcaatcgtc gccgcgcgcg
     9961 acgctatcgc atgagcgtgc ccttcaacgt ctcgctcttc gacccgaaac tgaaccgcga
    10021 acaactcgaa cacgcgcctt atctcgaagg cacgaccagc gacctcagcg cgagcgggct
    10081 tggtttgatc gttcccgcca tacgcattga cgaccgttac ctcaccgcgc cggatcaaac
    10141 cttacacgtc ctgctggaat ttccttccgg ccagatcgag atgcgcgtcg cgcccgtccg
    10201 ctacgaacaa ctcgaccggg aaggcgcacc gcagggcggc tatctcatcg gcgtgagcat
    10261 cctcgaaatg agcgacgacg cccgcacacg cttcgagcaa tatctgaaag aattgaccta
    10321 gcgaaatgtt cggtaaaatt taaggacgta aagaattttt agttcagggt gtgtccgaca
    10381 cggtttagtc tttgaagagt agccagccga acttttccgg cacgtggaag tttggttcga
    10441 gcgcatgagt cggctgccat gcgagatagc cgcgcgtgct gcctgcgccg atgcagcgaa
    10501 acaggttcac gcgccaacgc tcgcccgcac gcggcgcttc cacgacgccg aaagcctccc
    10561 atggcatgca catcgccaac gtgatcgaat caggttcgac gcgcgccgct gccttcatcc
    10621 cggaagagaa gtcccattcg gtttcacgcc cctccgccgt cgtgtgcagg gcgagatcga
    10681 gccactcgcc cgtcggcgca acttcaaatt caaaataacg ctccacctcg tctgcgcgcg
    10741 gcgcgacgaa gatctcgcac acgtctcgat cccacagccc tatcgtcttt cgctcgcgct
    10801 gcggactcgc gttcacaatc aaaggctcgc gctgcgcgca cacgaagcgc acgtacaaag
    10861 cacgctcgtc ccacagcagg cgcgcctccc cctgacgttc gggcggcgcg atctcgcccg
    10921 accagtaacg cgtgagatgc acgggacgcg cgcgcgccca ctccgcatga tccagttcag
    10981 ggacactcaa attcgaccac gcggagcgcg cgcgacctgc ttcgactgtt gtttccatgt
    11041 tcatcgtagc gcgggatgat agttgaagcc gtgacttttg ttaatcggtc gagagatgaa
    11101 tcttcgtggc ggcgtcgcgg atggcggcgt cgctcgtggc ggaaggcgag tcttccggcg
    11161 tcgaagtttg cggcgcgcgc gcgggttctc tgttccacca gcgcgcggtg gcgaaccaga
    11221 ggaggccggg caggaggaag aggatgccga tgccgatggt gacggcccgc ggcggatagt
    11281 tgaagcggtc gagcagttcg cccgtgacgt agtttgaagc ggccatcgtg agcgtgagca
    11341 gcgcgagttc ggcggcgaag acgcggccac gaaagttgtc ttcgatggcg gtttgcaaaa
    11401 gcaccgtgga gaagacccag aggatgctgc cgcccatgtg cgcgatgagc agcatgagca
    11461 gcgcgagcgc gtagttggtc gcgccgccga aggccgcgta gaagacgccg ccgacgagaa
    11521 acgagaggcc gatgatcgtc tgcatgcgcg tgcgcgactc gcccgcgaga cggcgcgcga
    11581 agacggggcc gagggccgtg ccgatgccgc gcgcggcgta gagcacgccg atccctgtgg
    11641 cggcggtttg cccgacaggg aagactttct ccccgaagac ggcgagcagc gcgagtatgc
    11701 cgccgcccaa tccccaggcg ggcttcaccg ccagcaaagc gagcacgcgc gggcgcgtcc
    11761 gcacgtagcg gatgccttcg agcgtgtccg tgatgccgag cgctttgctg atggtgagcc
    11821 gggtcttttc gcgcggcggg cgagggggga ggcgtatgcg ccagatgagt gcggcggaga
    11881 gcaggtaggt ggcggagtcc acgacgaagg cggcgtccgt gccgaaccaa cccgtcacga
    11941 ggccgcccgc caccgcgccg agcgtcagca tcgcagacca cgtgacggag gtgatcgcgt
    12001 ttgccgtgac gagatcgcgt tcggcgacga tggaaggaat ggcggctgat tttgccggtt
    12061 cgaaaaaggt ggagaaggtg agttgcagca ccgtgagcgc gtagatgagc cagatttgat
    12121 ccgggcggcg cacgaacaga aaaccgagca cgacgacggc gcgcaggatg tccgagacga
    12181 gcatcacggt gcgccggttg aacctgtccg cgacgacgcc ggagagtggt ccaacgacga
    12241 cgctcggcag aaaacgcgcc accatgatca acccgacatc gcgccccgac ccggtgaggt
    12301 tcaacacgag cgtcatcacg gcgatggtat cgaaccaatc gccgagttgg ctcaccacct
    12361 gccccaacca cagttgccgg aagccgcgat tctgccgcag cagttctaaa tagcccataa
    12421 gaaaacagtg acgagtgaca ggtgacaggt gacaagttga agacaagtaa atcgtgaatc
    12481 gtgaatcgtg aatagagaaa gactgtcttc ttctattcac gattcacgat tcaaggcttt
    12541 cactcgtcac tcgtcacctg tcactggtca ctgttttttg agagtgcgcc agccgggaga
    12601 gggttgtcaa cgtgttgtgt gtgcgggggc gtaaaaaaac tggaagtgaa aactggaagc
    12661 cgcctgttgc tctttgaagc gagcacgcgt ctcagtcagg aattgaccgc ccgtgtcagc
    12721 atgacctgtc aggttgacgg cttcgaggcg ggcgcgtcgc acggagttgt ccgttgctct
    12781 cgtgcgcggc gcgcttttgt acagttttgc gctgtttggc tcgcgtgcgg cgcgcggcac
    12841 gcggcttgtt taaggaatgg cgcggcttga agcgttaacg cgcttcttcg gactcaccgc
    12901 acggagtccg aaaagccgca ccacttttga caacctgtaa cggtcgcgcc cgaacgcgca
    12961 gcgcgaccgc aagcgggatc agattctcac ccctgacgcg tcgagtgagt gccacgcacc
    13021 cgacgcttgc ccacaccgcc gttgaagacg cgaggacgat ttgctcccgt ccggcgtctt
    13081 caacggcggg cttttttcga gcggcatcga gagatgcttt cagggtcgaa tcagtgaaac
    13141 ctgacgaccg ggaacaattg ccgcgaacgt gtgcgggcga aggatggttt gcagatgata
    13201 gacatgtcgg attacagcag cgcagccaaa gcctactggc tcgcgctcgt ctcggcgggc
    13261 gcgctcatcg ccgcatgggc gctctctcag tgtctctctt tcactccggt gcagggcttg
    13321 cagtttgtcg ccctcacgag tttcgtcgtc ctctcaagtt ctctccccat tcgcatcccc
    13381 aacacttcgg tcagcgtcac ggcgggcgac gccttcatct tcctcggcac gatcctgctc
    13441 ggcgtgcccg ccgctctcct gctcggcgca accgacgcgc tcgtcagatc gttcggcagt
    13501 tcgcgccgcg cgacgagttg gttcgccgcg cccgcgacga tgtctctgac cgtgctcgtc
    13561 tcggccagcg ccttctattt cctgctcaac tcttatgcgg gcgtcgcgcg ccaacccgtc
    13621 ggcatggcgc gtctgacctt cgaacaactc ctcgtgccgc tcgtggcgat ggcgctcctg
    13681 caatacttct tcaacgggat gctgctcgcc acgctgcacg cgctcaaaga gcagagcacg
    13741 atctggaagt tctggcgcaa cggctatctc tggacttcat gcactttctt cgccgccgcc
    13801 atcgcctccg cgctgctcta tctggcgatg cagaatttcg gcctcatcta cgtcctcttg
    13861 agcgtccccg tcatcggcgc aactttcgca acttataaga tctacttcga gcgcatcaac
    13921 gagcgttcgc gtgagacgga ggagatggcg cggctgcatc tcgccaccgt cgaggctctc
    13981 gccacggcga ttgacgcgaa agaccagacg acgcattgcc acgttcgccg cgtgcaactt
    14041 tatgcggcgc gcatgggcga gattctccat ctctcgcaca gcgagatcga agccctgaga
    14101 gccggggcga tgctgcacga catcggcaaa ctcgccgtgc ccgatcatat tttgaacaag
    14161 ccggggaaac tcacgaccgc cgagtttgaa cgcatgaaag ttcacaccat cgtcg
//
LOCUS       DASWNS010000331         4762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_401657, whole genome shotgun sequence.
ACCESSION   DASWNS010000331 DASWNS010000000
VERSION     DASWNS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401657"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..786)
                     /locus_tag="VGB76_11635"
     CDS             complement(154..786)
                     /locus_tag="VGB76_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099591.1"
                     /translation="MMAVVNITRLVRPVEETEEPPALHARAMDNLRFIRETMENAASF
                     TAVSGWGLCAVGLTAFVACYLAVRATERGAWIKIWIAEAVLSLFISVLAMSRKARAAR
                     QPLLSKPGRKLLFNLTPPFFVGALLTIVLSRYGTVRVIPAMWLLLYGAGVVTGGAFSV
                     RIVPVMGLCFMLVGTLALFSPAAWADAYMAAGFGGLHVVFGLLIARRHGG"
     gene            898..2694
                     /locus_tag="VGB76_11640"
     CDS             898..2694
                     /locus_tag="VGB76_11640"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0099592.1"
                     /translation="MHEVPAEIVELPASRRRCEFHASLASKACASLASEARASLALEA
                     RVSLKCAPSFTLRPQFVSILRVPHPATTQALRAKERKRALASAVEELLRARPARCRRS
                     GSSVGDMNEREPGELNSLKETTEVLQHEKLPRGITFVAEADGIREYRLANDLKTLLVE
                     NRVAPVATVCVLYKVGSRNEAVGYTGSTHLLEHMMFKGTPTFNKDRNTQIAATLQKIG
                     ADFNATTWYDRTNYFETVPSDKLELALRLEADRMRNSFIADADRQSEMTVVRNELERG
                     QNEPSMVLDEAVYATAFREHPYHHPTIGWRADVENVPTARLKAFYDTFYHPNNATLIV
                     VGDFERAATLELINEHFGAFDATHESIPEVYTDEPQQQGERRLVIKRAGELALVQIAF
                     HTPAVLGQSNVLSNAQLAERAANPPAENDIYPLVVLSAAMSNGVTSRLYQALVETELA
                     VSVTSNCDQFRDPGLFNVYATVNPGVEPRAVEEAILAELERAGREGLPDAEVQKAKQQ
                     IIAQVAYNRDGTSDIASQMSEAEAVADWRFYKDYAANIERVAAADVQRVARTYFAEDN
                     RTVGYFIPKAANSNGGADVEAGEAGADGSSDS"
     gene            2884..4302
                     /locus_tag="VGB76_11645"
     CDS             2884..4302
                     /locus_tag="VGB76_11645"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0099593.1"
                     /translation="MTKREGVGEDVSAGESTTMQELGQQPLAERGDKSSDAAEANTPA
                     SVTRSNFAARVARSSLPTGATLLVLENRATPTISLRGSLRAGSYFEPRDKPGLARLTA
                     DMLERGTRRRTKLQLANDLESVGAQIEFSADPFSVQIAARSLAKDLSLLLATLAEQLR
                     EPAFPAEELEKLKQQAIAAIQEQQANTRLRAYEKFSQLTYDPAHPFYVHAGDVLVKSL
                     NSITVEDVRRFYDERYGGRSLILSVVGDVQIDEVQRLFAEAFGEFTGPESVEVEVADP
                     EPSEAARREIVLLKEKANVDVLLGSAAPLRRTARDYYAATLANGALGESTLSSRLGLQ
                     VRDREGLTYGIGSRFRAPSLAAGPWYIAVSVNPTNVERAVNSALTVLRDYVTHGMTPD
                     ELADEKSSAIGSFKVSLSTNAGLAEALWNAEFYGLGLDYPDRFPELIQAVTLEEVNAA
                     IRKYFRPDQLTVVIAGDYEAQG"
     gene            4418..>4762
                     /locus_tag="VGB76_11650"
     CDS             4418..>4762
                     /locus_tag="VGB76_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0099594.1"
                     /translation="MRRRKGDGCPVAGALQLVGDKWTLLVVRDLARAPRRTTELISAL
                     HPISSRTLVGRLRDLERDSLVERRDYGGSPPHVEYALTERGLLLLPMVEALRQLGLSL
                     GCNECVDRRERLG"
BASE COUNT          958 a         1586 c         1395 g          823 t
ORIGIN      
        1 cgttcgtgaa tcaagcgatc caactccgtc gcgctccgct caggcgcatc ggcgctcgcg
       61 ttccccgccg ccttcgtcga ttgctcgacc gcgcgcaggt cgccctgccg cgccgctgcc
      121 gtcgccgtcc gtccttttct ctgtgcgctt cctttaacca ccgtgtctcc tcgcgatgag
      181 caggccgaag acgacgtgca gaccgccgaa gccggcggcc atgtaagcgt cagcccatgc
      241 cgccggactg aagagcgcga gcgtgccaac cagcatgaag cacaagccca tgaccggcac
      301 aatcctgacg gagaacgcgc cgcccgttac cactcccgct ccgtagagca acaaccacat
      361 cgcaggtatc acgcgcaccg tgccgtagcg cgagagcacg atggtcaaga gcgcgccgac
      421 gaagaacggc ggcgtgagat tgaagaggag tttgcgcccc ggtttcgaaa gtagaggctg
      481 ccgcgccgca cgcgccttac gactcatcgc caacaccgag atgaagagcg acagcacggc
      541 ctctgcgatc cagatcttga tccatgcgcc gcgctccgtc gcccgcaccg cgaggtagca
      601 ggccacgaac gccgtcaagc ccacggcgca caagccccaa ccggagacag ccgtaaacga
      661 cgcggcattc tccatcgtct cgcggatgaa gcgcaggtta tccatcgccc gcgcgtgcag
      721 ggcgggcggc tcctccgtct cttcaaccgg gcgcacgagg cgcgtgatgt taacgacagc
      781 catcatgcgc aagatacgcc ttcccgccgg ttccgtcaag tactttgcaa tgcaaagcca
      841 caaagaatat ttcgacctgc gaaccgtggc gaaaaagatt caacgcagag agagagtttg
      901 cacgaagttc cggcagagat cgtggagttg cctgcttcgc gccgccgttg cgagttccac
      961 gcatctctcg cgtcgaaggc ttgcgcgtct ctcgcgtcgg aggctcgcgc gtctctcgcg
     1021 ttggaggctc gcgtgtctct caagtgcgcg ccgagtttca cactccgccc gcagtttgtt
     1081 agcatactgc gcgtcccaca tcccgctaca acgcaggcgc tgcgcgctaa agaacgcaag
     1141 cgtgctctcg cctctgccgt cgaagaactg ctgcgcgcgc gtcccgctcg ttgccgacga
     1201 tccggcagca gcgtcggcga catgaacgag cgggagccgg gcgagttaaa ttcattgaag
     1261 gagacaacag aagttttgca gcatgaaaaa ctcccgcgcg gaatcacctt cgtcgccgaa
     1321 gcggacggca tacgcgagta caggctcgcc aatgatctga agacgctttt ggtggaaaac
     1381 cgcgtcgcgc ccgtggcgac cgtgtgcgtg ctctacaagg tcggctcgcg caacgaggcc
     1441 gtcggctata ccggctccac gcatctcctc gaacacatga tgttcaaagg cacgcccacc
     1501 ttcaacaaag atcgcaacac gcagatcgcc gccacgctcc aaaagatcgg cgcggacttc
     1561 aacgccacga cctggtacga ccgcaccaac tacttcgaga cggtgccgag cgataagctc
     1621 gaactcgccc tccgcctcga agccgaccgc atgcgcaact ccttcatcgc cgatgcggat
     1681 cgtcaatcgg agatgaccgt cgtgcgcaac gaactcgaac gcgggcagaa cgaaccctcg
     1741 atggtgctcg acgaagcggt ctatgctacg gccttccgcg agcatcctta tcatcatccg
     1801 accatcggct ggcgcgccga cgtggaaaac gtgcccaccg cgcggctcaa agccttctac
     1861 gatactttct atcatccgaa caacgccacg ctcatcgtcg tcggcgactt cgagcgcgcc
     1921 gcgacgcttg agttgatcaa cgagcacttc ggcgcgttcg atgctacgca cgagtccatt
     1981 cccgaagtct atacggacga accgcagcag cagggcgagc ggcgtctcgt gatcaagcgc
     2041 gcgggcgaac tcgcgctcgt gcagatcgct tttcacacgc ccgccgtgct cggacagtcg
     2101 aacgtgcttt caaacgcgca actcgccgaa cgcgccgcca acccgcccgc cgaaaacgac
     2161 atctacccgc tcgtcgtcct gtcggcagcg atgtcgaacg gcgtcacgag tcgcctctat
     2221 caagctctgg tcgagacgga actggccgtg agcgtcactt cgaactgcga tcagtttcgc
     2281 gaccccggac tcttcaatgt ctacgcgacg gttaatcccg gcgtcgagcc gcgcgcggtt
     2341 gaagaagcga tcctcgcgga gttggagcgc gccgggcgtg aaggtttgcc tgacgcggag
     2401 gtgcagaagg cgaagcagca gatcatcgcg caggtcgctt acaaccgcga cggcacttct
     2461 gacatcgcct cgcagatgtc cgaggccgaa gccgtcgccg actggcgttt ctacaaagac
     2521 tatgcggcca acatcgagcg cgtcgccgcc gcagacgtgc agcgcgtcgc gcgcacctac
     2581 tttgccgaag acaaccgcac cgtcggctac ttcatcccga aggcggcgaa cagtaacggc
     2641 ggcgcagacg tagaggcggg cgaagctggc gcagacgggt cgagcgactc ttgatcgagc
     2701 gtcgcgtgct caagcctcgc gcatgcttgg acagaatcta aaattcttcg cgtccacgcc
     2761 tctctttctc tgcgcccttg cgtgaaatgt tcttctcgca aaagcgcaag gttggagcgg
     2821 aaggcgcaac aactcggcat tcaaactcgt gtttgagttc atcgtgggat gggagtgaaa
     2881 gagatgacga aacgggaagg cgtgggagag gacgtgtcgg cgggcgagag cacgacgatg
     2941 caggagttag ggcagcaacc gctcgcggag cgcggcgata aatcctcgga cgccgccgaa
     3001 gcgaacacgc ccgcgagcgt gacgcgctca aacttcgccg cgcgcgtcgc acgcagttct
     3061 ttgcccacgg gcgcgacgct tctcgtcctc gaaaatcgcg ccacgccgac catctccctg
     3121 cgcggcagtc tgcgcgccgg ttcatatttc gagccgcgcg ataagcccgg cctggcgcgt
     3181 cttaccgccg acatgctcga acgcggcacg cgtcgccgca cgaagctcca actcgcaaac
     3241 gacctcgaat cggtcggcgc acaaatagaa ttctccgcag accctttcag cgtgcagatc
     3301 gccgcccgct cgctcgccaa agatttgtcg ctcctgctgg cgacgctggc ggaacagtta
     3361 cgcgagcctg cgtttcccgc agaagagttg gagaagttga aacagcaggc catcgcggcc
     3421 attcaggaac agcaggcgaa cacgcgcctg cgcgcctacg aaaagttttc tcaactcact
     3481 tatgatccgg cccacccgtt ctacgtccac gccggagacg tgctggtcaa aagcctcaac
     3541 tcgatcacag tcgaagacgt acgacgcttc tacgatgaac gatacggcgg gcgttcgctc
     3601 atcctctccg tcgtcggcga cgtacagatc gacgaagtgc agagactgtt cgcagaggcg
     3661 ttcggcgagt tcacaggtcc ggagagcgtc gaggtcgagg tggccgatcc cgaaccttct
     3721 gaagcggcgc gacgcgagat cgtgttgctc aaggagaagg ccaacgtaga cgtgctgctc
     3781 ggttcggctg cgcctttgcg ccgcaccgcg cgcgactact atgcggccac gctcgccaac
     3841 ggcgcgctcg gcgaatccac gctctcttcg cggctcggcc tacaggtgcg cgaccgcgaa
     3901 ggcttgacct acggcatcgg ctcacgcttt cgtgcgccct cgctcgccgc cggtccatgg
     3961 tacatcgccg tctccgtcaa tcccacgaac gtcgagcgcg cggtcaactc cgcgcttacc
     4021 gtcttgcgcg actacgtgac gcacggcatg acaccggacg aactcgcaga cgaaaaatca
     4081 tccgccatcg gctccttcaa agtctcgctc tcgaccaacg cggggttggc cgaagcgctc
     4141 tggaacgcgg agttctacgg cctcggtctg gattacccgg atcgcttccc cgaactcatt
     4201 caagccgtca cactcgaaga ggtcaacgcc gcgattcgca aatattttcg tccggatcaa
     4261 ctcaccgtcg tcatcgcggg cgactacgaa gcgcagggat gaaggataag ggatgaatca
     4321 gaaaagcact cgatcagttg aaggcttttc tgattcatcc ttcatccctc atcctttgct
     4381 ttgtgttaat ctgtgcgcgc gaaggagaga agcgagtatg cgacgacgca aaggcgacgg
     4441 ttgccctgtg gcgggcgcgc tacagttggt cggcgataag tggacgctcc ttgtcgtgcg
     4501 cgatctggcg cgtgcgccgc gccgcacgac cgaactcatc agcgcgctcc acccgatcag
     4561 cagccgcacg ctcgtcgggc gactccgcga tctggaacgc gactcgctcg tcgagcgccg
     4621 cgactacggc ggcagcccgc cgcacgtcga gtacgcgctg accgagcgcg ggctgctgct
     4681 gctaccgatg gtcgaagccc tgcgtcaact cggcctctcg ctcggctgca acgaatgcgt
     4741 agaccgccgc gagcgtctcg gc
//
LOCUS       DASWNS010000332        11091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_167395, whole genome shotgun sequence.
ACCESSION   DASWNS010000332 DASWNS010000000
VERSION     DASWNS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167395"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1083)
                     /locus_tag="VGB76_11655"
     CDS             complement(97..1083)
                     /locus_tag="VGB76_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY0099595.1"
                     /translation="MRAIKIVAHGGVEGLEIGEVEEPLATGDRVRVRVRAAALNRADL
                     LQRRGHYPAPPGAPASIPGLEFAGEVEALGTEASRWRVGERVFGITGGGAQAEYVVVP
                     ENTLAAVPGNLSWEEAAAVPEAFITAHDALFTQGGLALGERVIVHAAGSGVGLAAVQL
                     ARAAGAQTFGTARTAGKLERARAYGLDEACVVGDDPQAFAAAVRAWTNDAGVQLILDL
                     VGANYLAANLDVLASRGRLLLVGTLAGASAPLDFRRVMGKRLRITGTVLRARSPEEKA
                     RATRLFAAHVSPLLARGALRPVIDSVYDLTDIRAAHLRLESNETFGKIVIKV"
     gene            1232..2242
                     /locus_tag="VGB76_11660"
     CDS             1232..2242
                     /locus_tag="VGB76_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0099596.1"
                     /translation="MTDRTETFVVVNYNAARARVAWTQIERVLRERGVRFRAHEAKEA
                     GDAQHAAREALRAGYGTIAVVGGDGTLGEVVSGFFARETGGVHTTQATTTTTPARIND
                     EAALAILPAGTGNDFARGLEGGRASLERWLERLVEHSLRGDGERTTRAVDVLHGSIGE
                     EDARQTFFCLNAATLGIGAEVAGRVAAQGDAVRRLPGEARFALAAIKSLASWRRRRVR
                     IRLDDAEAFECETNLVAVANSPFAGGGMMFSPGARVDDGLLDVVTASRLTRASLIREM
                     TRIHSGGHVKNPHVSIARAVRVRIEHLTDGDALAVEADGDVRGHTPLEFRVLAGALRV
                     VV"
     gene            complement(2370..2831)
                     /gene="rpiB"
                     /locus_tag="VGB76_11665"
     CDS             complement(2370..2831)
                     /gene="rpiB"
                     /locus_tag="VGB76_11665"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEY0099597.1"
                     /translation="MKIAIGADHGGYPLNEHIIEILRAAGHEIEDFGTHDGTRADDYP
                     DYALQVGEAVRAGRAACGIIICGSGVGASVAANKLRGIRAALCGDTYSARQSREHDDC
                     NVLCLGARVVGIELATEIVRAFVGASFTGEERHRRRLAKVLDIERRETTNE"
     gene            complement(2887..4323)
                     /locus_tag="VGB76_11670"
     CDS             complement(2887..4323)
                     /locus_tag="VGB76_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099598.1"
                     /translation="MRKLIFLLLLLCPFSLTHGTAQKNVRRDAANAVDVYAVVAEVER
                     IAAARTLWPNFDPRRVPVEIYDGQTTLLFQHPAPPPEFVSLSSRQGVWAFPGRHETVT
                     ANAPVKLNNVLTATVMFKPGGRESLRERAALVVHETFHVFQRERHPKWAGDELELFVY
                     PFDDAELLALRRLETEALRRADEARRKLPDARCWARLALTTRRERFARMRAGAVTYER
                     ATELNEGLARYVEARAAGTHTANLPAGEYPSTDLRTRAYATGHAFALLLERFAPVWTT
                     RLEAGENLSLDELLNAALSQESAPASNADNKAASSDASASSRACALPGSFAETARRRA
                     GVEVASLQEQRATKRREFEAQGGWKIVVKADAGAPLWPQGFDPLNVLRLGLREVLHTR
                     YLKLGNDAGAIEILDRHSLTEGAGAEHPLWGGVRQLTLAGITEEPAAHEAAGKTTITA
                     ANIKGEFRGAQLERAGQTLSITLQSASK"
     gene            complement(4379..6430)
                     /gene="tkt"
                     /locus_tag="VGB76_11675"
     CDS             complement(4379..6430)
                     /gene="tkt"
                     /locus_tag="VGB76_11675"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0099599.1"
                     /translation="MSASTQPTATSPEAELDELCINTIRTLSLDAVQRAESGHPGLPL
                     GAAPMAYVLWTRFLRHNPQHPKWQNRDRFLLSAGHGSMLVYSLLHLTGYDLPLEELKN
                     FRQWESKTPGHPEYGLTPGVEITTGPLGQGFANGVGMAMGAAHLASKFNREGFPVIDH
                     FVYAIVSDGDLMEGVAAEAASLAGHLKLGKLIYLYDDNRVTIEGFTSLAFTEDVPKRF
                     ESYGWHTSTVEDGNDLAAIERAIREAQAETTCPSLISVKTVIGFGMPAAGTRKAHSDA
                     PGAEAVKATKRALGWPEDEEFHLPEAALTHFRAAIERGQRAEDEWNALVERYTEAHAE
                     QGRMWRDLMSGELPAGWEEKLPTFADAKPVATRVASGEVINALAGVLPSLIGGSADLG
                     VSNNTDIKEGGSFQPGSYDGRILHFGVREHAMGATLTGISLNGGLVPYGGTFLTFSDY
                     MKPAIRLAALSEVQVVYVFTHDSVGLGEDGPTHQPIEHLAGLRAIPNLYVIRPADPHE
                     TREAWRIAIGRRHAPTALILTRQKVPVLDQTLYSPATGVQRGAYILAEAAGAEGRNAT
                     PQLIIIATGSEVALALGARAELQKEGIPTRVVSMPCCELFDAQEASYREEVLPSNITA
                     RLAVEAGASLGWARYVGLAGDCVCLDRFGASAPGDVALRNLGFNVENVTQRARALLRR
                     G"
     gene            complement(6539..8077)
                     /locus_tag="VGB76_11680"
     CDS             complement(6539..8077)
                     /locus_tag="VGB76_11680"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEY0099600.1"
                     /translation="MSQSDGRIEAALARAPYVVALDIGTSSGRAALFDADGREIEATG
                     ARVEWSFKTTREGGAEIDANWLLAQTARILDETLARVAGLKLPVEAVAVACFWHSLVG
                     VDAEGRALTPLYGWADTRAALEVAELRRRFDERATHARTGCRFHASYWPAKLLWLREE
                     KPDVWREDVRWLSFGEFLTLRFCGVGAASVSQASATGLFDQRRAVWDEELCAALALDV
                     NQLPPLAREGNAAFHVAGEYAARWPQFKGRPWFAAVGDGATNNIGAGCVNRNSLALMI
                     GTSGAMRVLYEGEPPAALPESLWCYRADRRRVLVGGALSDGGGLYDWMRDNLRVGNVE
                     NPDAPHADDEEIARQVAALAPDAHGLTVLPFWAGERSTNWNSQARGAIIGLTMHTRPP
                     EIMRAALEAVAYRFAQIARALEAFAPGAEVRASGGALAASPLWAQMLADVLARPVKLS
                     NAREASSRGAALLALESLGVIDELTDAPAELCRTFEPDMQAHALYLAGAARQEIIYQS
                     LLKD"
     gene            complement(8084..8638)
                     /locus_tag="VGB76_11685"
     CDS             complement(8084..8638)
                     /locus_tag="VGB76_11685"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HEY0099601.1"
                     /translation="MQSKRGDERLAITLSGSPSDPTGSEGLSNATGDFMAADSKDSRA
                     LCADFQANIDEITLEWVRRVKSDAHMRSDDHLTTAQIVDHVPQMLEELCTLFEHAGVP
                     DFHTIRASSQHGYMRSLEGYSLVDLLRELELLRDCVFDFVAETELKAHVNRRETIRAL
                     QTVNHYFGEDIIFVVEHFLKNRNK"
     gene            complement(8628..10127)
                     /locus_tag="VGB76_11690"
     CDS             complement(8628..10127)
                     /locus_tag="VGB76_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0099602.1"
                     /translation="MNRQNFFGHLALAAAVVILAAFIGQMRRMERSHDSSARRTVVVR
                     HDETDHDADEAGDFRSEQGDAEVIVRFRPGTSPQAINRITEGLNDRLLDRFEFINGQA
                     VIADEDGLSPETVAAEYRSLPEVEYAEPNFVIDLDPAERAGELDDKGEVNEEVEVEDD
                     DDPPVVRGSDKGDGQQPNDPLFKEQWSLLNTGQRSGRSGADVCATRAWAKTQGTRDVV
                     VAVIDTGVDYKHQDLHNNIWTRPASLAPYVDAELGEFDDRHGFDAADNDGDPMDDNGH
                     GTHCAGIIGAEGDNEDGIAGVNWKVEIMGLKFLSRNGSGTTKDAIECINYVVARKKAG
                     VNVRIISASWGSTAHSRALGDAIKRAGEEGILFIAAAGNSSTDNDRLPHYPSNYQHPN
                     VVSVAALTRKDVLAGFSNYGAKTVHIAAPGAEIMSTWPQNMYEEHSGTSMATPVVSGV
                     AALILSVNPDLSVTNLRKRLLETVDKIPALDGKVSSGGRVNAARAVRAE"
     gene            complement(10169..10444)
                     /locus_tag="VGB76_11695"
     CDS             complement(10169..10444)
                     /locus_tag="VGB76_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099603.1"
                     /translation="MAMSKEDAVQRARTDLAGRLGVTKDEIKEDSVEDADFADAAFGA
                     GTADEVSAQMITPGWRIRLRAGRDVYEYRGNRNRLRLFNFKGANYSV"
     gene            complement(10701..10991)
                     /locus_tag="VGB76_11700"
     CDS             complement(10701..10991)
                     /locus_tag="VGB76_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099604.1"
                     /translation="MSTHIKRVLSQRFLLAISLVLTMIAFSLVLAQPPKADASPKAGA
                     ATTACPSSTIEYTYYTDASMTVECGWRIITCYCGGGSGGCRTAYYTIDWSEC"
BASE COUNT         1888 a         3487 c         3624 g         2092 t
ORIGIN      
        1 taaatagacg aagaaaaaat ttctcgattc accatttacg attcacgatt tactgttctc
       61 tctcgtcacc tgtcacccgt cacttgtcac tactcttcac actttaataa ctatcttgcc
      121 gaaggtttcg ttggattcga gacgaaggtg tgcggcgcgg atgtctgtga gatcgtagac
      181 gctgtcgatg acggggcgga gcgcgccgcg cgcgaggagc ggcgagacgt gcgcggcgaa
      241 gaggcgtgtg gcgcgcgcct tctcttccgg cgagcgtgcg cgcaggacag tgcccgtgat
      301 gcgtagtcgt ttgcccatca ctcgccggaa atcgagcggc gcgctcgccc cggccaacgt
      361 gccgacgagc agcaggcgac cgcgcgacgc aagcacgtcg aggtttgccg cgaggtagtt
      421 tgcgccgacg agatcgagta tgagttggac gcccgcatcg ttcgtccaag cacgcacggc
      481 ggcggcgaac gcctgcgggt cgtctccgac gacgcacgct tcgtcgagtc cgtaggcgcg
      541 tgcgcgttcg agtttgcctg cggtgcgtgc cgtgccgaac gtctgcgctc cggcggcgcg
      601 tgcgagttgg acggcggcga gtccgacgcc cgaccccgct gcgtgaacta tgacgcgctc
      661 gcccaacgcc aacccgccct gcgtgaagag cgcatcgtgc gccgtgatga aagcctccgg
      721 cacagcggcg gcctcctccc aactcagatt gccgggcacg gcggcgagag tgttttcggg
      781 gacgaccaca tactcggcct gcgccccgcc gcccgtgatg ccgaacacgc gctcgcccac
      841 gcgccacctc gaagcctccg tccccaacgc ttcgacctcg cccgcaaact ccaaccccgg
      901 aatactggcg ggcgcaccgg gcggcgcggg gtaatggccg cgtcgctgca acaggtcggc
      961 gcgattcaac gccgccgcgc gcacacgcac gcgcacgcgg tcgcccgtcg ccagaggttc
     1021 ttcaacctcc ccgatctcca aaccttcaac cccgccatga gcgacaatct tgatagcacg
     1081 catgaatgaa tcgtaaatcg tgaatcgcaa atagacaaaa gacgtaaacg tgaatcgtca
     1141 atcgtcaata gattcgagat ggtcttctcg attcacgatt tacgattcac gatttacgat
     1201 tcatagttta caggttaatg tcttgccccg catgactgat cggacagaga cattcgtggt
     1261 cgtcaactac aacgccgccc gcgcgcgcgt cgcatggacg cagatcgagc gtgtgctcag
     1321 ggagcgcggc gtgcgcttcc gcgcgcacga ggcgaaggag gcgggcgatg cgcaacatgc
     1381 ggcgcgcgaa gccctccgcg cgggttacgg gactatcgcc gtcgtcggcg gcgacggcac
     1441 gctcggcgaa gtggtgtcgg gtttttttgc acgcgagacg ggaggcgttc atacgacgca
     1501 ggcgacaacg acaacaacgc ccgcgcgcat caacgacgaa gccgcgctcg ccattctgcc
     1561 cgccgggacg ggcaacgatt tcgcgcgtgg cctcgaaggc gggcgcgctt cgctcgaacg
     1621 ctggctcgaa cgtctcgtcg agcatagcct gcgaggcgat ggggaacgca cgacgcgcgc
     1681 cgtagatgtg ctgcacgggt caatcggcga agaagatgcg cgccagacct tcttctgcct
     1741 caacgcagcg acgctcggca tcggtgcgga agtggcgggg cgcgtcgcgg cgcagggcga
     1801 tgcggtgcgg cgtcttccgg gcgaagcgcg tttcgcgctc gcggcgatca aatcgcttgc
     1861 gagttggcgt cggcgacgcg tgcggattcg gttggacgac gcggaggcgt ttgagtgtga
     1921 gacgaatctg gtggccgtcg ccaacagccc gttcgcgggc gggggcatga tgttctcgcc
     1981 gggggcgcgc gttgacgatg ggctgctcga tgtcgtcacc gccagccgtc tcacgcgcgc
     2041 ctcgctcata cgcgagatga cgcgcatcca cagcggcggc cacgttaaga acccgcacgt
     2101 ctccatcgcg cgcgccgtgc gcgtccgcat cgaacacctg accgacggcg acgcgctcgc
     2161 ggtcgaagcc gacggcgacg tgcgcggcca cacgccgctt gagtttcgcg tcctcgcggg
     2221 cgcgctcagg gtcgtcgttt aggaaagccg cccttccgtc cgtggtcagt tgcccgttgc
     2281 ctgtgatctt gaagctgaac ggtatcgctc gaagttgaga gcggggggcg caaaacagcg
     2341 gacaagcgag cacggacaga ggacacgctt cattcgttgg tcgtctcgcg gcgttcgatg
     2401 tcgagcactt tggcgaggcg gcgacggtgt cgctcttcgc ccgtgaagct tgcgccgacg
     2461 aaggcgcgca cgatctcggt ggcgagttcg atgccgacga cgcgtgcgcc gagacagagg
     2521 acgttgcagt cgtcgtgctc gcgcgattga cgcgccgagt aggtgtcgcc gcataacgcc
     2581 gcgcggatgc cgcgcagttt gttcgccgcg accgatgcgc cgacgccgct cccgcagatg
     2641 atgatgccgc aggcggcgcg tccggcgcgc acggcctcgc ccacctgcaa ggcgtaatca
     2701 ggatagtcgt ctgcgcgtgt gccgtcgtgc gtgccgaagt cttcgatctc gtggcctgcc
     2761 gcgcgcagga tttcgatgat gtgttcgttg agcggatagc cgccgtggtc tgcgccgatg
     2821 gcgatcttca ttccagcctc tccctgcgga caaaaatttt aacgcgccgc tctcacttct
     2881 tacttcttac ttcgacgctg actgcaaagt gatgctcagc gtctgtcccg cgcgttcgag
     2941 ttgtgcgccg cgaaactcgc ctttgatgtt ggcggcggtg atcgtcgtct tgcccgcagc
     3001 ttcgtgcgcg gcgggttctt cggtgattcc ggcgagcgtt aactggcgca cgccgcccca
     3061 gagcggatgc tccgcgcctg cgccttcggt gagcgagtgg cggtcgagaa tctcaatcgc
     3121 gcccgcgtcg ttgccgagtt tgagataacg cgtgtggagc acttccctca gccctagccg
     3181 gagcacgttc agagggtcga agccttgcgg ccagagcggt gcgcccgcgt ccgccttgac
     3241 gacgatcttc caaccgccct gcgcctcaaa ctcgcgccgc ttcgtcgccc gttgctcctg
     3301 caaacttgct acttcaactc cggcacgcct gcgagccgtt tccgcgaacg accccggaag
     3361 cgcgcaggcg cgggaggaag cggaggcgtc ggaagaggcg gccttgttgt cggcgttgct
     3421 ggcgggcgcg gattcttgag acagagcggc gttgagaagt tcgtcgagcg acaggttctc
     3481 tccggcttcg agtcgcgtgg tccagacggg cgcgaagcgt tcgagcagga gggcgaaggc
     3541 gtgtcccgtc gcgtaggcgc gcgtgcgcag gtctgtggag ggatattctc cggcaggcag
     3601 gttcgccgtg tgcgtcccgg cggcgcgcgc ttccacgtag cgcgcgagtc cttcgttgag
     3661 ttcggtcgcg cgttcataag tgaccgcgcc tgcacgcatg cgcgcgaagc gttcgcgtcg
     3721 cgtggtgagc gcgagccgcg cccaacaacg tgcgtccggc agcttccggc gcgcttcgtc
     3781 ggcgcgacgc agagcttcgg tttcgagacg acgcagcgcg agcagttccg catcgtcgaa
     3841 cgggtagacg aacagttcca gttcatcgcc cgcccacttc ggatgacgct cgcgctggaa
     3901 gacgtggaac gtttcgtgta cgacgagcgc ggcgcgctca cgcagcgact ccctgccgcc
     3961 gggtttgaac atgacggtcg ccgtcaacac gttgttcagt ttgacgggcg cgttggctgt
     4021 gaccgtctcg tgccgaccgg gaaacgccca cacgccctga cgcgacgaga gcgagacgaa
     4081 ctccggcggc ggcgcgggat gttgaaagag cagcgtcgtc tgcccgtcgt agatctccac
     4141 gggcacgcgg cgcggatcga agttcggcca gagcgtacgc gctgccgcga tgcgttcgac
     4201 ttcggcgacg acggcgtaaa cgtccacggc gttcgccgca tcgcgccgca cgttcttctg
     4261 cgccgtcccg tgcgtcagag agaacgggca gagcaggagc aggaggaaaa ttagtttgcg
     4321 cataacggca attcccccga cgacgcgcga aagttccaca aaggcgggaa cgccggactc
     4381 agccacgccg cagcagcgca cgcgcgcgtt gtgtcacgtt ctcgacgttg aagccgagat
     4441 tgcggagcgc gacatcgccg ggagcggatg cgccgaagcg gtcgagacag acgcagtcgc
     4501 ccgccaagcc gacgtagcgc gcccaaccca aactcgcgcc cgcttcgacg gcgagccgcg
     4561 cagtaatgtt tgaaggcagg acttcttcgc ggtagctcgc ctcctgcgcg tcgaacaact
     4621 cgcagcaggg catcgagacg acgcgagttg gaatcccctc cttctgcaac tccgcgcgtg
     4681 cgccgagcgc gagcgcgact tccgagccgg tggcgatgat gatgagttgc ggcgtcgcgt
     4741 tccggccttc cgcgcctgcg gcttcggcga ggatgtacgc gccgcgttgt acgcctgtag
     4801 ccgggctgta aagcgtctgg tcgagcacag gcactttctg gcgcgtgagg atgagagcgg
     4861 tcggagcatg gcgtcgcccg atagcgatgc gccatgcttc gcgcgtctcg tgcgggtcgg
     4921 cggggcggat gacgtacagg ttcgggatgg cgcgcagccc cgcgagatgc tcgatgggtt
     4981 gatgcgtggg gccgtcttcg ccgaggccga cggagtcgtg cgtgaagacg tagacgacct
     5041 gcacttccga gagcgcggcg agacggatgg cgggcttcat gtagtcggag aaggtgagaa
     5101 acgtgccgcc gtaagggacc agcccgccgt ttaaactgat gcccgtcagc gtcgcgccca
     5161 tcgcgtgctc gcgcacaccg aaatgaagga tgcgcccatc gtaagagccg ggctgaaaac
     5221 tgcccccctc tttgatgtcc gtattgttgg agacgccgag gtctgccgag ccgccgatga
     5281 gcgaaggaag cacgcccgcg agcgcgttga tcacttcgcc cgaagccacg cgcgtcgcca
     5341 ccggcttcgc gtccgcaaac gtgggcaact tctcttccca accggcgggc agttcaccgc
     5401 tcatcaagtc gcgccacatg cgaccctgct ccgcgtgcgc ttccgtgtaa cgctcgacga
     5461 gcgcgttcca ttcatcttct gcacgctggc cgcgctctat cgccgcgcgg aaatgtgtga
     5521 gggctgcttc gggcaggtga aactcttcgt cttccggcca gccaagcgcg cgcttcgtcg
     5581 ctttgacggc ctctgcgccg ggcgcgtccg agtgcgcctt gcgcgtgcct gcggcgggca
     5641 tgccgaagcc gatgacggtc ttgacggaga tgagcgacgg gcacgtcgtc tcggcctgcg
     5701 cttcgcggat cgcgcgctcg atggctgcga gatcgtttcc gtcttcaacg gttgaagtgt
     5761 gccagccgta agactcgaaa cgcttcggca cgtcttctgt aaaagcgagc gaagtgaagc
     5821 cttcgatggt gacgcggttg tcgtcgtaga gatagatgag cttgccgagt ttgagatggc
     5881 ctgcgagcga ggcggcctcc gccgcgacgc cttccatcag gtcgccgtcc gagacgattg
     5941 catagacgaa gtgatcgatg acggggaagc cttcgcggtt gaacttcgaa gcgagatgcg
     6001 ccgcgcccat cgccatgccg acgccgttag cgaagccctg accgagtggt cccgtcgtga
     6061 tctcgacgcc gggcgtcagt ccgtattcgg gatggccggg agttttcgat tcccactggc
     6121 ggaagttttt caactcttcg agcgggagat cgtagcccgt caggtgcagc agcgaataga
     6181 cgagcatcga gccgtgtccg gcggagagca ggaagcggtc gcggttctgc cacttcgggt
     6241 gctgcgggtt gtgacgcagg aagcgcgtcc agaggacgta agccatcggc gcggctccga
     6301 gcggcaggcc ggggtggccg gactcggcgc gctgcacggc gtcgagcgag agcgtgcgaa
     6361 tggtgttgat gcaaagttcg tcgagttccg cttcgggcga agtggcggtc ggttgtgtgg
     6421 acgcactcat ctttaaagtt tcctctgtgt gtttgggttc aagtatgaat gattgcggtt
     6481 gcgccgccgg ttctgttgct tcgtgccggt ggcattcgtg ccggtgaccg tcgaacgtct
     6541 aatctttcaa gagcgattgg taaatgatct cttgccgcgc tgcgcccgca aggtagagcg
     6601 cgtgcgcctg catgtccggt tcgaacgtgc ggcagagttc ggcgggcgcg tcggtcagtt
     6661 catcaatcac gcccaaagat tctaacgcaa gcagggctgc gccgcgactc gacgcctcgc
     6721 gcgcgttcga aagtttaacg gggcgcgcga gcacgtccgc gagcatctgc gcccagagcg
     6781 gcgaggccgc gagcgcgccg cccgaagcgc gaacttcagc gccgggcgca aaggcttcga
     6841 gcgcgcgtgc gatctgcgcg aagcgatagg cgacggcttc gagcgcggcg cgcatgatct
     6901 ccggggggcg cgtgtgcatc gtcagtccga tgatcgcacc gcgcgcctgc gaattccagt
     6961 tcgtgctgcg ctcgccagcc cagaagggca ggacggtcag gccgtgcgcg tcgggcgcga
     7021 gcgcggcgac ctgacgcgcg atctcttcgt cgtcagcgtg cggcgcgtca gggttttcga
     7081 cgttcccgac tcttaaattg tcgcgcatcc aatcgtagag gccgccgcca tcggagagcg
     7141 cgccgccgac gaggacgcgc ctgcggtcgg cgcgatagca ccataaactt tcgggcaacg
     7201 cggctggcgg ctcgccttcg tagagcacgc gcatcgcgcc ggaggttccg atcatcaacg
     7261 cgagcgagtt ccggttgacg caacccgcgc cgatgttgtt ggtcgcgccg tcgccgacgg
     7321 cggcaaacca cgggcgaccc ttaaactgcg gccagcgtgc agcgtattca cccgcgacgt
     7381 gaaaggcagc gttgccttca cgggcgagcg gcggcagttg attcacgtcg agggcgaggg
     7441 cggcgcaaag ttcttcgtcc cacacagcgc gtctctgatc aaacaatcct gtcgccgaag
     7501 cctgcgagac gctcgccgct ccgacgccgc agaagcggag cgttaaaaat tcgccgaacg
     7561 agagccagcg cacgtcttcg cgccatacgt ccggcttctc ctcacgcaac cagagcaact
     7621 tcgcaggcca gtagctcgca tgaaaacggc aacccgtgcg cgcatgcgtc gcgcgctcgt
     7681 cgaagcgacg gcgcaactcc gcgacttcca gcgcggcgcg cgtatcagcc caaccgtaga
     7741 gcggcgtgag cgcgcgccct tccgcgtcaa cgccgacgag gctatgccag aagcaggcga
     7801 cggcgacggc ctcaaccggc aacttcaacc ccgcgacgcg cgccagcgtc tcatcgagga
     7861 tgcgcgccgt ttgtgcgagc agccagttgg cgtcaatctc cgcgccgcct tcgcgcgtcg
     7921 tcttgaaact ccattcgacg cgcgccccgg tcgcttcaat ttcgcgcccg tcagcatcga
     7981 acaaggccgc gcgtccgctc gacgtgccga tgtcgagcgc gaccacgtag ggcgcgcgtg
     8041 ccagcgcagc ttcgatccgc ccgtcactct gagacacggt tcttcacttg ttacggttct
     8101 ttaaaaaatg ttcgacgacg aaaataatat cttcgccgaa atagtggttg acggtttgca
     8161 gcgcgcggat ggtttcgcgg cggttgacgt gagctttaag ttccgtctcg gcgacgaaat
     8221 cgaagacgca atcgcgcagg agttcgagtt cgcgcaggag atcgacaagc gagtagcctt
     8281 ccagcgagcg catgtagccg tgctggctgg aggcgcggat ggtgtggaag tcgggcacgc
     8341 ctgcatgctc gaagagcgtg cagagttctt cgagcatctg cgggacgtga tcgacgatct
     8401 gcgccgtcgt caaatgatcg tcggagcgca tgtgcgcgtc gcttttcacg cgccgtaccc
     8461 attcgagcgt gatctcgtcg atgttcgctt gaaagtccgc gcacagcgcg cgggaatctt
     8521 tggaatcggc ggccatgaaa tctcccgtcg catttgaaag cccctccgat ccggttgggt
     8581 cggaggggct gccggagagt gttatcgcaa gtcgctcgtc tccgcgttta ctctgcacgc
     8641 acggcgcgcg cggcgttgac gcgtccgccg cttgagactt tgccgtcgag cgcagggatt
     8701 ttgtccaccg tctcaagcag acgcttgcgc aggttcgtca cggagagatc gggattgacc
     8761 gaaaggatga gcgcggcgac gccggagacg acgggcgttg ccatcgaagt gcccgaatgc
     8821 tcctcgtaca tgttctgcgg ccaggtgctc atgatctccg cgccgggagc ggcgatgtgc
     8881 acggtcttcg cgccgtaatt ggagaagccc gcgagcacgt ccttgcgcgt gagcgcggcg
     8941 acgctgacca cgttcgggtg ctggtagttg gaaggatagt gcggcaggcg gtcgttgtcc
     9001 gtgctggaat ttcccgccgc cgcgatgaag agaatgccct cttcacccgc gcgcttgatg
     9061 gcatcgccga gcgcacgcga atgcgccgtc gaaccccaac tcgcgctgat gatgcgaacg
     9121 ttgacgcccg ccttctttcg cgccacgacg tagttgatgc actcgatggc atctttcgtc
     9181 gtgccgctgc cgttgcggct gaggaacttc aaccccatga tctcgacctt ccagttgacg
     9241 cccgcgatgc cgtcctcgtt gtcgccttcc gcgccgatga ttccggcgca gtgcgtgccg
     9301 tgtccgttgt cgtccatcgg gtcgccgtcg ttgtctgctg cgtcgaagcc gtggcggtcg
     9361 tcgaactcgc cgagttcggc gtcaacgtaa ggcgcgagcg aggcgggacg cgtccagatg
     9421 ttgttgtgca aatcctgatg cttgtagtca acgcccgtgt cgatcacggc gacgacgaca
     9481 tcgcgcgtgc cctgcgtttt cgcccacgcg cgcgtggcgc acacgtccgc gcccgatctg
     9541 cccgaacgct gcccggtgtt caagagcgac cactgctctt tgaagagcgg atcgttcggc
     9601 tgctgcccgt cgcctttgtc gctgccgcgc acgacgggcg gatcgtcgtc gtcctcaact
     9661 tccacttctt cgttgacttc gcctttgtcg tcgagttcgc ccgcgcgctc cgccggatcg
     9721 agatcgatga cgaagttcgg ctcggcgtat tcgacttcgg ggaggctgcg atattcagcc
     9781 gcgaccgtct cgggcgagag gccgtcttcg tctgcgatca cggcctgccc gttgatgaac
     9841 tcgaaacgat ccagcaggcg gtcgttgagt ccttccgtga tgcggttgat cgcctgcggc
     9901 gaagtgccgg gacggaagcg cacgatcact tccgcgtctc cctgttcgga gcgaaaatcg
     9961 cccgcctcgt cagcgtcgtg atccgtctcg tcgtgcctga cgacgacggt gcgccgtgca
    10021 gacgaatcgt gcgaacgctc catgcgccgc atctgcccga tgaaggccgc gagaatgacg
    10081 actgccgctg ctaacgccag atgcccgaaa aaattctgcc ggttcattgc cttacctctt
    10141 ttgctgtggg aaaaaccggg tgtgccggtt agacgctgta gtttgcgcct ttgaaattga
    10201 agagtcgcag gcgattgcgg ttgccgcgat attcgtaaac gtcacggcct gcgcggaggc
    10261 ggatgcgcca tcccggcgtg atcatctgcg cgctcacttc gtccgctgtg cccgcgccaa
    10321 acgccgcatc cgcaaaatcc gcatcctcaa ccgaatcttc tttaatctca tctttcgtta
    10381 cgccgagccg ccccgcgaga tcggttctcg ccctctgcac agcgtcctcc ttactcatag
    10441 ccatacgccc gacgctcctt caaggtttct ctgccagcgg ccaaaacagc gactaaagat
    10501 gaactcggcg cgagacttct tcgaccacac ctgccgacct cgttcgcgca ctcatttgcg
    10561 acgatccata ttctgcgctt actcggggcg attcgtcaaa aaatttaagc ggcggagtct
    10621 tacccaaact ccgccgctct ttcgacgcga tctttcaaca cgatctttta cgcatcgtga
    10681 ggaatcatcc gggacgagcg ttagcactcc gaccagtcaa tcgtgtagta agcagttcga
    10741 cagccgccgc tgccgccgcc gcagtagcag gtgatgatcc tccagccgca ctcaaccgtc
    10801 atcgaggcgt cggtgtagta ggtgtactcg atggttgaag aggggcaggc agttgtcgcc
    10861 gcacccgcct tcggggaggc atccgctttc ggcggctgcg ccagcacgag gctaaaggca
    10921 atcatggtca ggacgaggct gatggctaac aggaagcgtt ggctgaggac tcttttaatg
    10981 tgagtggaca tgagtggttc tccttacgtg ggttcagtcg cctgccgtct gtgggcggtg
    11041 tggctgaggt tagagacgtg aggccggtga aagcatagag gatgcgtccc g
//
LOCUS       DASWNS010000333         5493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_375742, whole genome shotgun sequence.
ACCESSION   DASWNS010000333 DASWNS010000000
VERSION     DASWNS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375742"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2481
                     /locus_tag="VGB76_11705"
     CDS             <1..2481
                     /locus_tag="VGB76_11705"
                     /inference="COORDINATES: protein motif:HMM:NF039178.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HEY0099605.1"
                     /translation="NGSNALPVEPGRYSVVASVTDPNFRGGASATLTIYAPGAVPLKA
                     FPGAEGAGELARGGRGGDVYHVTNLNDSGAGSLREGIRTATGARTIVFDVSGTINLNS
                     RLNINRAFLTLAGQTAPGDGITVAGWPTVINTNNVIVRYMRFRVGDLRCPAVQDDALW
                     VDKSKDVILDHVSASWSVDETLSVTDSEGVTVQWSFITESLKNSCHEKGAHGYGSLIR
                     GGNGLVSYHHNLYAHHDSRNPRLGDNVGLDFVNNVVYDWGGEAGYSGEASEGAPRLNY
                     VGNYLVAGPSTPASKRNRAFNGGSTATQIYQSGNLIDSDLDGQRDGADTDWSMIIGSY
                     TRRQPARFDFAQIQTHDARTAYERVLSTAGHSLARDAVDARIANDVRNGGGSHINSQS
                     QVGGFPELEAAQASADTDGDGMPDGWESERGLNPADPADGRNANASGYTNLETYLNDI
                     VPVPGVEPGADQTPPVTLAALSREANAAGWHNSDVTVTLGSADEEGGSGLREIIYAVN
                     GLLNFAPGGAVSIPVSNEGTTTITYYSRDEAGNPEAAQTLTVRLDKTAPVVSDVTRTA
                     PNANGWNNTDVSVTYATTDALSGFDSGHSFSGVSSLTTEGLGQTVAFEVSDLAGNTAG
                     GTVSGINIDKTAPTVGLVQPAEGGLYLLGQTTHADYACADNLSGTDCSGTVASGASFD
                     TSSVGVKTFGVSATDLAGNNATVQVNYIVGYGLGLLYDADKAKRSGSTIPFKLQLLDA
                     SGANRSAAGSIVHVVGVSPPAGQPLSPVEDAGRANSGGNFRYDAALGGTGGYIFNLRT
                     AGYAPGTYLLHFTVGQDPYIYSTPFRIE"
     gene            2614..3072
                     /locus_tag="VGB76_11710"
     CDS             2614..3072
                     /locus_tag="VGB76_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099606.1"
                     /translation="MSTIRIGVGRGRASGVYGRCWYPTAERPLYRITCQLPGPFPARI
                     PTRQPPVYRNADGTWPPLPAGCTEGLSCRAEKDGVVREWKRVIGYTEVRTRDEGLVWI
                     VAHEAFHFLRHTRQIKGRNTEIEADRFADEQLRLLRKLRERWTIGSGAHL"
     gene            complement(3429..4301)
                     /locus_tag="VGB76_11715"
     CDS             complement(3429..4301)
                     /locus_tag="VGB76_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099607.1"
                     /translation="MKWIPPKSGNSLEGLPTENHLLMNRQTVSPELTRSYLLGELDPE
                     ARRQLEETLMVEEEYYKELLIAEDELIDRYLSGGLDAHEQERFLQHFLTTPGRRQKLD
                     FARTFQTYVTNHTPQPSAPERGGREPRSDRARLLPLSARGYHRALRLTFTAAMLVAVL
                     GGSWVLWKRWQQPAPSGGAEHRVLVVSLSPILTRSVGADRPIFIPANITTVQLQLEPT
                     VGGQESYSAKLLTDEGREVYNANALTAKSDGSVVFAVPAELLEGGDYRVKLEGSKGGA
                     TEGAGIYHFRAGRE"
     gene            complement(4298..4855)
                     /locus_tag="VGB76_11720"
     CDS             complement(4298..4855)
                     /locus_tag="VGB76_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099608.1"
                     /translation="MNKEWVLTQESFNQLLNWLAPNREQAARKYEEIRSRLIMIFTCR
                     GCYEAEELADETINRVTRRVPDIAGTYTGDQSLYFYGVAHKVHLEYLRKKPVLPPPPP
                     VERSEEDEIRYECLEQCMEHLTQENRELLLHYYHGEKQAKIDARREFAARLNIPLNAL
                     RIRAFRLRERLHACVLECMGKRAAG"
     gene            complement(4923..>5493)
                     /locus_tag="VGB76_11725"
     CDS             complement(4923..>5493)
                     /locus_tag="VGB76_11725"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0099609.1"
                     /translation="FATHGLLINDHPELSGIVLSLRNESGQPVDGFLRLHDIYNLDLP
                     VDLVVLSACSTALGKDIRGEGIVGLSRGFMYAGAASVLASLWRVDDEATAELMGHFYH
                     NLLQNDLPPSAALREAQLAMLRQKQWRAPYFWAAFVLQGEYRERLGARQHAPVAAFSR
                     KLLPTSLLFFLIGGGLLVIHRKRRRAKNI"
BASE COUNT         1051 a         1806 c         1628 g         1008 t
ORIGIN      
        1 aacggctcga acgcgctgcc cgtcgaaccg ggacgctaca gcgtcgtcgc ctccgtcaca
       61 gaccccaact tccggggcgg ggcttcggcc acgctcacca tctatgcccc cggcgcagtg
      121 cccctcaagg cattccccgg cgcggagggc gcgggcgaac tcgcgcgcgg agggcgcggc
      181 ggcgatgtct accacgtcac caacctcaac gactccggcg cgggcagcct gcgcgaaggc
      241 atccgcacgg ccacaggtgc tcgcaccatc gtcttcgacg tgtcgggcac gatcaacctc
      301 aactcgcgtc tgaacatcaa ccgcgcgttc ctgactctcg ccggacagac cgcccccggc
      361 gacggcatca cggtcgcggg gtggccgacc gtcatcaaca ccaacaacgt catcgtgcgc
      421 tacatgcgct tccgcgtcgg cgacctgcgc tgccctgccg tgcaggacga cgcgctctgg
      481 gtggacaagt cgaaagacgt gatcctcgat cacgtctccg cctcctggag cgtggacgag
      541 acgctgtcgg tgacagactc cgaaggcgtc acggtgcaat ggtctttcat caccgagagc
      601 ttgaagaact cctgccatga gaagggcgcg cacggctacg gctcgctcat tcgcggcggc
      661 aacggcctcg tctcttacca tcacaatctc tacgcgcacc acgacagccg caacccgcga
      721 ctcggcgaca acgtcggcct cgactttgtc aacaacgtcg tctacgactg gggcggcgag
      781 gccggttaca gcggcgaggc gagcgagggc gcgccgcgtc tcaactacgt cggcaactac
      841 ctcgtcgcag gcccttccac tcccgcctcc aagcgcaatc gcgccttcaa cggcggcagc
      901 acggccactc aaatctacca gtcgggcaat ctcatcgact ccgacctcga cggtcagcgc
      961 gacggcgcag acacagactg gtctatgatc atcggcagct acacgcgccg tcagccagcc
     1021 cgcttcgact tcgcgcagat tcagacgcac gacgcccgca cagcctacga gcgcgtgctc
     1081 tctacggcag gccactcgct ggcgcgcgat gccgtagacg cgcgcatcgc aaacgacgtg
     1141 aggaacgggg gcggctcgca catcaactcg cagagtcagg tcggcggctt cccggagctt
     1201 gaggccgcgc aggcgtccgc cgacacagat ggcgacggca tgcccgacgg gtgggagagc
     1261 gagcgtggac tcaatcccgc agaccccgcc gatggccgca acgcgaacgc cagcggctac
     1321 accaacctcg aaacatacct gaacgacatc gtccccgtgc cgggcgtcga accgggcgca
     1381 gaccagaccc cgcccgtcac gctggccgcc ctctcgcgcg aagcgaacgc ggctggctgg
     1441 cacaattcgg atgtcacagt cacgctcggc tccgccgacg aagagggtgg ctccggcttg
     1501 cgcgagatca tctacgccgt caacggcctt cttaacttcg cacccggcgg ggcggtttcc
     1561 atccccgtca gcaacgaggg cacgacgacc atcacctact actccagaga cgaggcgggc
     1621 aaccccgaag cggcgcagac cctcacggtc aggctcgata agacggcacc cgtcgtcagc
     1681 gatgtgacgc gcaccgcgcc aaacgctaac ggctggaaca acacggatgt ctctgtcacc
     1741 tatgcaacga ccgacgcgct ttccggcttc gactcgggac actctttcag tggcgtgtcg
     1801 agcctgacca cagagggtct ggggcagacc gtcgccttcg aagtctcaga cctcgcgggc
     1861 aacactgcgg gcgggacggt cagcggcatc aatatcgaca agaccgcgcc caccgtcggc
     1921 ctcgtgcaac ccgccgaagg cggcctctac ctcctcggcc agacgacgca cgcagactac
     1981 gcctgcgccg acaacctttc gggcacagac tgctccggca cagttgcttc cggcgcatcg
     2041 ttcgacacgt cctcggtcgg cgtcaagacc ttcggcgtca gtgccacaga cctcgccggg
     2101 aacaatgcca ccgtgcaggt caactatatt gtcggctacg gcctcggtct gctctacgat
     2161 gccgacaagg cgaagagaag cggcagcacg atcccgttca agctgcaact gctggacgcc
     2221 tcaggcgcga atcgttccgc tgccggatca atcgtgcatg ttgtgggcgt gtcgcccccg
     2281 gcgggacagc ctctcagccc ggtcgaagat gcgggtcgtg ccaactccgg cggaaacttc
     2341 cgttacgacg cggcactcgg cgggacgggc ggctacatct ttaacctgcg cacagcgggt
     2401 tacgcccccg gcacatacct tctccatttt accgtcgggc aagacccata catctactcg
     2461 acgccgttcc gcatcgagtg atcgcacact catgcggcaa ggtagagagg tttcgttatt
     2521 cagacacagt gggtagtcga agttgatcgc cgggaggtcg aaagcggtct ggactgggcg
     2581 cgtgatctct tcgcgcgtta cgacacctcg gagttgtcta cgatacgcat cggcgtagga
     2641 cgcgggcgtg cgtcgggcgt ctacggtcgt tgctggtatc cgacggctga gaggccgctc
     2701 tatcgcatca cgtgccagtt gcccggcccc tttccggcgc ggataccgac gaggcaaccg
     2761 cccgtctacc ggaacgcgga cggcacttgg ccgccgcttc ccgcaggctg cacggaagga
     2821 ctctcgtgtc gtgcggagaa agacggagtc gtgcgcgagt ggaagcgcgt gatcggttat
     2881 acagaggtgc gtacgcgcga cgaaggcttg gtctggatcg tcgcgcacga ggcttttcac
     2941 tttctccggc acacgcgcca gatcaaggga cgcaatacag agatcgaggc cgatagattc
     3001 gccgacgaac aattgcgtct cttacgcaaa ctgagagagc gttggacgat tggctccggg
     3061 gcgcacctct gagcgtgagc attacacggt cgagtgatcc gaagttgcgc ccgcgcccgg
     3121 cgcatttatc aggctaagcc acatcgcgcc gcacaaactc aagacaaccc ccggcgcgtt
     3181 tcaatcatgt atttttgcat cttcgccccc gacgtgataa acggctcaac acttgatcca
     3241 ttaacacgcg ttcgggaaag actcgcggcg tcgagcgagg caaaaggaga gactgtttta
     3301 gttgaggtta ttcccggcgc caacggcggc gggtggttat tcgtcgaaaa ccctgcgagg
     3361 tcgcggcata gttgaggcgg cggtttgaat cgctgcgcgc ttcagttcaa ctcctgccga
     3421 ccggcggttt attcgcgccc ggctctgaaa tggtagatgc ccgcgccttc tgtcgcgcca
     3481 cctttgctgc cctccagttt cacacggtag tccccgccct ccaacaactc tgccggaacc
     3541 gcgaagacga cggagccgtc gctttttgcc gtgagcgcat tcgcgttgta gacctctctg
     3601 ccctcatccg tcaggagctt cgcgctataa ctttcctgac cgccgaccgt cggttcgagt
     3661 tggagttgga cagttgtgat gttcgccggg atgaagatcg gcctgtccgc tccaacgctt
     3721 ctggtcaaga tcggagagag agagacaacg agcaccctgt gctctgctcc gccggacggg
     3781 gcaggctgtt gccaacgctt ccagagcacc cacgaaccgc cgagcaccgc caccaacatc
     3841 gcagccgtga aagtgagacg gagcgcgcgg tgatagccgc gcgcggaaag gggcaacagg
     3901 cgcgcccggt cggagcgcgg ctctctcccg ccgcgctccg gggctgaggg ctggggcgtg
     3961 tggttcgtca cgtaggtctg gaaagtgcgc gcgaagtcga gtttctgacg ccgcccggga
     4021 gtggttagaa agtgctgaag aaatctctcc tgctcgtgcg cgtcgagtcc gccgctcagg
     4081 taacggtcga tcaattcgtc ttccgcgatc agaagctctt tgtaatactc ttcttcaacc
     4141 atgagtgtct cctcaagctg acggcgcgct tcggggtcta gctcgccgag caagtacgac
     4201 ctggtgagtt cgggtgatac cgtctgtcta ttcatgagaa ggtggttttc cgtgggcaac
     4261 ccttccaagc tgttaccgga tttcggcgga atccatttca tcccgcggct ctttttccca
     4321 tgcactcaag cacgcacgcg tggagcctct cccgcaggcg gaacgccctg atccggagcg
     4381 cgttcagcgg gatgttcaga cgcgcggcaa actctctgcg ggcatctatt ttcgcctgtt
     4441 tctccccgtg gtagtagtgc aacagcagtt cacgattctc ctgcgtcaaa tgctccatgc
     4501 actgttccag acattcatac ctgatctcgt cctcttcgct ccgctcgacc ggcggcgggg
     4561 gcggcagcac aggctttttt cgcaggtact ccagatgcac cttgtgcgcg acgccgtaaa
     4621 agtagagaga ctggtcgccc gtgtacgtgc ccgcgatgtc cggcacacgg cgcgtcacgc
     4681 ggttgatcgt ctcgtcggct aattcttccg cctcgtaaca accgcgacag gtgaagatca
     4741 taattagtcg gctgcgaatc tcttcatact tcctcgccgc ctgttcacgg ttgggtgcca
     4801 gccaatttag caactgattg aagctttcct gtgtcagaac ccattctttg ttcatgcggg
     4861 tgaatgttaa cgtctccggc tcccacgtgc ccggcggacg tgcgatgttc agggatgaat
     4921 agttaaatgt ttttcgcgcg ccgccgcttg cggtgaatga caaggaggcc gcccccgatg
     4981 agaaaaaaca gcaggctcgt cgggagcagt ttacgggaga aggctgcgac cggggcgtgc
     5041 tgccgcgccc ccagcctctc cctgtattca ccctgcaaca cgaaggcagc ccagaagtaa
     5101 ggtgcgcgcc actgcttctg tctcaacatc gcaagctgcg cttcacgcaa agccgccgag
     5161 ggcggcagat cgttctgcaa aagattgtga tagaagtgtc ccatcaactc tgccgtcgcc
     5221 tcgtcgtcaa cgcgccacag gctcgccaac acactggccg cgcccgcata catgaagccg
     5281 cgcgacagcc cgacgatgcc ttcgcccctg atgtcctttc cgagcgcggt gctacacgcg
     5341 ctcaacacca ccagatcaac gggtaagtct aagttgtaaa tgtcgtgcag gcgtagaaag
     5401 ccgtcaaccg gctggccgct ttcgttcctg agcgagagca cgatgccgga caactccggg
     5461 tgatcattaa tgagcaggcc gtgggtggcg aaa
//
LOCUS       DASWNS010000334         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_39080, whole genome shotgun sequence.
ACCESSION   DASWNS010000334 DASWNS010000000
VERSION     DASWNS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39080"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VGB76_11730"
     CDS             complement(<1..854)
                     /locus_tag="VGB76_11730"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY0099610.1"
                     /translation="MFYRSVVRPVLFRLAPETAHEFALHALTVALKPESARRLAASRL
                     SRAPFGKLERFGLSFSNPVGLAAGFDKNGAAVRELAALGFGFIEVGTVTHLAQAGNPQ
                     PRLFRLPKDRALINRQGFNNEGAAALAGRLRRARPGCVLGINIGKSRVVEVRDAVADY
                     LASFEIVYPHADYITVNVSSPNTPGLRELQRADALTELLAALQTRNGELARHPELSSG
                     GVRTSAGRVPLLLKVAPDLDERALELIVDVARRTGIDGIIATNTTTERTNLLQTPPGV
                     VSAIGAGG"
     gene            1022..1606
                     /gene="pyrR"
                     /locus_tag="VGB76_11735"
     CDS             1022..1606
                     /gene="pyrR"
                     /locus_tag="VGB76_11735"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY0099611.1"
                     /translation="MLVSAPMEFTEKVRIMDGARINRALARLASEIVEENQGSSDLYL
                     VGIQRRGVPLAHRLADKIADLEGDRPPVGIVDITLYRDDLSTVGANPIVNRTDLPGGV
                     DDRNIVLIDDVLYTGRTIRAALDQLIDFGRPRRVQLAVLVDRGKEHRELPIQADFVGK
                     FAPTKRSEIIKVMLTEFDGEEGVAIVERPATENE"
     gene            1745..2821
                     /locus_tag="VGB76_11740"
     CDS             1745..2821
                     /locus_tag="VGB76_11740"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY0099612.1"
                     /translation="MALASRHLLGISELSPAEIVHILDTAETFQAISQREIKKVPTLR
                     GRTVINLFFEASTRTRTSFELAGKRLSADTINISASTSSVTKGETLLDTARNLEAMSP
                     DFIVIRHPSAGAPHALARICRAAVINAGDGAHEHPTQALLDALTIRQHKGRIERLRVA
                     IIGDILHSRVARSNIQLLTKLGAEVRLAGPGTLVPAELKELVSQGELRVVSRIEEAIE
                     GADVVMVLRIQRERQGAAFFPSVREYAVHYGLNLKRLGGAQADAIVMHPGPMNRGIEI
                     ASDVADGTRSLILDQVSNGLAVRMAVLYLLAGGSVLNQNMLAQTKPETATAVDAQTTS
                     TTTRTSSVEQLEPVAAGQTKSKDK"
     gene            2880..>3853
                     /locus_tag="VGB76_11745"
     CDS             2880..>3853
                     /locus_tag="VGB76_11745"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY0099613.1"
                     /translation="MKLLIANGYLIDPTQGLNGGKNLLIEDGRVAALPAHNDGVPDGA
                     EVFDATGLVVAPGFIDLHTHLREPGQEYKETIETGAAAAVAGGWTSVCAMPNTDPVND
                     NPAVTRFIIEQAERAGLANVFPLGAITKNSEGAELAEMGEMKRAGIVAVSDDGRPVPS
                     AGIMRRAMEYARGFDLPVVDHCEDRSLTAGGVMHEGNWSLRLGLRGHPALAEEMDALR
                     DIALAELTGARVHLAHVSTRGALEAVRRAKERGLDVTCEVTPHHWSLEDEAVRDYDTN
                     TKMCPPLRSRDHVEAIIEALRDGTIDAIATDHAPHHADEKALEYDQAP"
BASE COUNT          789 a         1243 c         1162 g          659 t
ORIGIN      
        1 aagccgcccg cgccgattgc cgagacgacg ccgggcggcg tctgcaaaag attcgttctc
       61 tccgtcgtcg tattcgtcgc gatgattccg tcaatccccg tgcggcgcgc gacatccacg
      121 atcaactcaa gcgcacgctc atccaagtca ggcgcaacct tcaagagcaa cggcacgcgc
      181 cccgcactcg ttcgcacgcc ccccgacgaa agctcaggat ggcgcgcgag ttcgccattg
      241 cgggtttgca gcgcggcgag caactccgtg agcgcgtcgg cgcgttgcag ttcgcgcaag
      301 cccggcgtgt tgggtgaact gacgttgacg gtgatgtagt ccgcgtgcgg gtagacgatc
      361 tcgaaactgg cgagataatc ggcgaccgca tctctaactt caaccacgcg gctcttgccg
      421 atgttgatgc cgagcacgca accggggcgc gcacgccgga gtcgcccggc gagcgcagcc
      481 gcgccctcgt tattgaagcc ctgacgattg atgagcgcgc gatccttcgg gagtcgaaac
      541 aggcgcggct gcgggttgcc cgcctgcgcc agatgcgtca ccgtccccac ctcgatgaac
      601 ccgaagccta gcgcagccaa ctcgcgcacc gccgccccgt tcttatcaaa ccccgccgcc
      661 agccccaccg gattactgaa actcagcccg aaccgttcca acttcccgaa cggcgcacgg
      721 ctcaaacgac tcgccgcgag acgcctcgca ctctccggct tcagcgcgac cgtcaacgcg
      781 tgcaacgcaa actcatgcgc cgtctccggc gcgagacgaa acaggacggg gcgcacaacc
      841 gagcgataga acatcagtga cgagtgataa gtgacgagtg acaagtgatg agaacgaaaa
      901 agagtttatc tcgtcacctg tcacttgtca ctcgtcaccc ttcttttgtc actcgtctct
      961 gtttccctgt tttcgctcct gtttttctcg tcgcttgtca ctcgtcaccc gtcgcttctt
     1021 tatgttagta tccgcgccga tggaatttac ggagaaagtt cggatcatgg acggggcgcg
     1081 catcaatcgt gcgctggcgc gtctggcgtc ggagatcgtg gaggagaatc agggttcgag
     1141 cgatctctat ctggtgggca tccagcggcg cggcgtgccg ctcgcgcatc gcctcgccga
     1201 taagatcgca gacctcgaag gcgaccgccc gcccgtcggc atcgttgaca tcacgctcta
     1261 tcgcgacgac ctttcaaccg tcggcgcgaa ccccatcgtc aaccgcacgg acttgcccgg
     1321 cggcgtggac gaccgcaaca tcgtcctgat cgacgatgtg ctctacacgg gacgcaccat
     1381 ccgcgccgcg ctcgaccagt tgatagattt cgggcgaccg cgccgcgtgc aactcgccgt
     1441 cctcgtggac cgcggcaagg aacaccgcga gttgccgatt caggcagact tcgtggggaa
     1501 gtttgcgccg acgaaaagga gcgagatcat caaggtgatg ctcacggagt ttgacggcga
     1561 agaaggcgtc gccattgtgg agcgaccggc gacggagaac gaataaaggt ggcggcctca
     1621 gttggccgcc tttttcgtgt caaagcgaaa gtcgcccgcg caggctggta aagaatagta
     1681 aagaatagcg aagatgaagt aagaattgag cagaccgtcc gcaacctgag aggcgcgttt
     1741 gaagatggct ttagccagca gacatttgct cggaatcagc gaactcagcc ccgcagagat
     1801 cgtgcacatc ctcgacacgg ctgagacttt tcaggccatc tcgcaacgcg agatcaagaa
     1861 agtgccgacc ctgcgcggtc gcaccgtcat caatctcttc ttcgaggctt ccacgcgcac
     1921 gcgcacctct ttcgaactcg ccggaaagcg tctctccgct gacaccatca atatcagcgc
     1981 atctacttcg agcgtgacca aaggcgagac gcttctcgac acggcgcgca acctcgaagc
     2041 gatgtcgccc gacttcatcg tgatacgcca tccttcggcg ggcgcaccgc acgcgctcgc
     2101 gcgcatctgt cgcgccgccg tcatcaacgc gggcgacggc gcgcacgagc accccacgca
     2161 agccctgctc gacgcgctca cgatccgcca gcacaaaggg cgcatcgaac gcttacgcgt
     2221 cgccatcatc ggcgacatac tccattcgcg cgtcgcgcgt tcgaacatcc aactcttaac
     2281 caaactcggc gcggaagtgc gtctcgcagg accgggcacg ctcgtgcctg cggagttgaa
     2341 agagttggtt tcgcagggcg agttgcgcgt ggtgagccgc atcgaagagg cgattgaagg
     2401 cgcggacgtg gtgatggtct tgcgcatcca gcgcgagcgg cagggcgcgg cgttcttccc
     2461 ctccgtgcgc gagtatgcgg tgcactacgg cctcaatctc aaacggctcg gcggcgcaca
     2521 ggcggacgcc atcgtgatgc accccggccc gatgaatcgc ggcatagaga tcgcgtccga
     2581 cgtggccgac ggcacgcgct cgctcatcct cgatcaagtc tcgaacggac tcgccgtgcg
     2641 catggccgtg ctctacctgc tcgcgggcgg ctccgtgctc aaccagaaca tgctcgcgca
     2701 gacaaaaccc gaaactgcga cggcagttga tgcacagaca acttcaacca caactcgaac
     2761 gtcttccgtc gaacaactcg aacccgtcgc cgccggacag acgaagtcga aagataagtg
     2821 acaagttcaa tcagcaaaca acaactgacc acggacaacc gactgccgac ctcccttcca
     2881 tgaaactgct catcgccaac ggctatctca tagaccctac gcaagggtta aacggcggaa
     2941 aaaatttact gatcgaggat gggcgcgtcg cggccttacc ggcgcacaac gacggcgtgc
     3001 cggacggcgc ggaagttttc gacgcgacgg ggcttgtcgt cgcgcccggc ttcatagatt
     3061 tgcacacgca cctgcgcgag ccggggcaag aatacaaaga gacgattgag acgggtgcgg
     3121 cggcagccgt cgcgggcggt tggacgagcg tgtgcgcgat gccgaacacc gatccggtca
     3181 acgacaatcc ggcggtcacg cgtttcatca tcgagcaggc ggagcgcgca gggcttgcca
     3241 acgtctttcc cctcggcgcg atcacgaaaa actcggaggg cgcggaactc gccgaaatgg
     3301 gagagatgaa gcgcgcggga attgtcgccg tctcggacga cgggcggcct gtgccgtctg
     3361 cgggcatcat gcgtcgcgcg atggagtacg cgcgcgggtt cgacctgccg gtcgtagatc
     3421 attgcgagga tcggagcctg acggcgggcg gcgtgatgca tgagggcaac tggtctttgc
     3481 gtctcggact gcgcggccat cctgcgctcg cggaagagat ggacgcgctg cgcgatattg
     3541 cgctcgcgga attgaccgga gcgcgcgtgc acctcgcgca cgtctcgacg cgcggcgcgc
     3601 tcgaagccgt caggcgtgcg aaggagcgcg gccttgatgt gacgtgcgaa gtgacgccgc
     3661 accactggtc gctcgaagac gaggccgtgc gcgactatga cacgaacacg aagatgtgcc
     3721 cgcctttgcg cagccgcgat cacgtggaag cgatcatcga ggctctacgc gacggcacga
     3781 ttgacgccat cgccacagac cacgcgcccc atcacgccga cgaaaaagct ctcgaatacg
     3841 atcaggctcc gtt
//
LOCUS       DASWNS010000335         3266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_222129, whole genome shotgun sequence.
ACCESSION   DASWNS010000335 DASWNS010000000
VERSION     DASWNS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222129"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..826
                     /locus_tag="VGB76_11750"
                     /pseudo
     CDS             206..826
                     /locus_tag="VGB76_11750"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
     gene            823..1716
                     /locus_tag="VGB76_11755"
     CDS             823..1716
                     /locus_tag="VGB76_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEY0099614.1"
                     /translation="MNAEQPHTSDAANAPSQERRGIRKGLYLIPSAFTAANIGMGYFA
                     VMGALRGFQLVEGGTEAELLQATSHFDNAARAIGWAVVFDALDGRIARLTKTTTEIGV
                     QLDSIADVVTFGIAPAVLAYAWGYGSAFAQGSNAHKLGWFLSFMFLICGAFRLARFNV
                     QATRPRVLAEGTVKVDKKNFVGLPIPMAGGFIAALVHFSPAPLSFHGIEMSTIYSGLL
                     MGAIALLSVLMVSTVRYTSFKTVGIGRRSTRLAVLAIAASGMLVWLYSRYVLVSLVGG
                     YIVYGLLTRLAGLFRHRSTHQ"
     gene            complement(1797..2966)
                     /locus_tag="VGB76_11760"
     CDS             complement(1797..2966)
                     /locus_tag="VGB76_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0099615.1"
                     /translation="MCERNHNNKYQGSRSLLYLLLPLFLLFPTKVYSAPFSISKVEKG
                     LIFSSPLMIRWQFDSHNTITLSPASSNSYIYLPLAAGEIVSLNAADGHLRWRTEIGGE
                     FSASPIADERGVYIASKAGETTNSSQVLRSTGAIRALSQSTGITLWMRTLHFPIQGGL
                     TSSATRLFGGSADGRIYAFNKATGEILWTVQHTSGFSSQPSLHQGRLYIGSEDGTLFA
                     LDESSGATLWRYRTRGALRGAVAVANNITYFGSADGYIYAFSEMNGRLLWRRRAGAAV
                     QAVVLTDSGIIVTSLDNFVYLLSPRRGELLWKRQLAGRIAALPLTDNRSALFSPLGGD
                     ACIVLSLKDGKQLNTLPVGEEGNTSATPLVVGELLIVPTRQGLIAFAPSSPNPNP"
BASE COUNT          720 a          895 c          900 g          751 t
ORIGIN      
        1 aagctggccg tgaaagatca gatgcaggag atcatgctcc gctaccagaa aaaagaagaa
       61 tccacgcatt gattctaccg tgcatcaatg gcgcgcaggt tagaagctag attcatcaga
      121 ttaaggaagg ggacgccgca gtagaaaaac gcgcgccccc tttttctctt tttcccgcag
      181 ccttcaagca aaagaacagg actcaaacgt gttacgagac ggcatcccct ttgtcttaat
      241 tccattggcg gcggccttgc tggctgctct cacaggctac tggtacatcg ccgtgccgct
      301 tctgctgctc gcgctcttca tggcctactt cttccgcgat cctgaacgcg cgccgctcac
      361 cgaagaagac ctcatcgtcg ctcccgccga cgggcgggtc acgcgagtca cacaggctat
      421 tccggacgat ccggagtcgc cgcaggtggt aagtattttt ttgtcggtct ttgatgtcca
      481 cgtcaatcgc gcgcccatcg caggcgagat cacggacgtg gcttacacga agggacgttt
      541 catgatcgcc acgcgcgaag aagcgagcgt cgttaacgaa cagaacgcac tgacgatcaa
      601 gggcgagagg atgaccgtcg tgtgcaaaca gatcgcgggc gtgctcgcgc gccgcatcgt
      661 gtgctggaag cgcaaaggcg agcgtgtgaa tttaggcgaa cgcttcggac tgataaagtt
      721 tagttcgcgc acagacctcg tgctgcctgc ggaagtcgac atcgccgtgc gcgtgggaga
      781 tcgtgtgcgc ggcggcgtca gcatcatcgg gaggttgcgt agatgaacgc cgagcaaccg
      841 cacacgtcag acgccgccaa cgctccctct caagaacgac gcggcatccg aaagggctta
      901 tatctgatcc cttcggcctt caccgccgca aatatcggca tgggttattt cgccgtgatg
      961 ggtgctttgc gcggctttca gttagtcgaa ggagggacgg aggctgaact gttacaagcc
     1021 acttcccatt ttgataatgc cgcgcgtgcc attggttggg cggtggtctt cgatgccttg
     1081 gacgggcgca tcgcgcgact cacaaagact actaccgaga tcggagtgca actggactcc
     1141 atcgcagatg tcgtgacctt cggcatcgcg cccgcagtgc tcgcttacgc ctgggggtac
     1201 ggttcggcct tcgcgcaggg aagcaatgca cacaagttgg gctggttcct ctccttcatg
     1261 tttctcatct gcggagcttt caggctggcg cgcttcaacg tgcaggcgac gcgtccgcgc
     1321 gtgctggctg aaggcacggt taaagttgat aagaaaaact tcgtcggctt accgatccct
     1381 atggccggtg gttttattgc cgccctcgtg cacttctcac ccgcaccact ctcctttcac
     1441 ggtatcgaga tgagcacgat ctatagcggt ctgttgatgg gggcgatcgc actgctctcc
     1501 gtgctcatgg tgagcacggt tcgctatacg agttttaaga cggtcggcat cggcaggcgc
     1561 agcacacgtc tggctgtcct cgccatcgcc gcctcgggta tgttggtctg gctctattcc
     1621 cgctacgttc tcgtctctct cgtaggtggc tacattgtct acggtttgct gacgcggctg
     1681 gccggtctgt tccggcatcg cagcacacac cagtgagacg cacgcccgac gctggagctg
     1741 cttttaggtt tggctgaaag tctaacccgc cccgaagaga tttcctttgg gtgtgattat
     1801 ggattggggt ttggagacga cggcgcaaaa gcaatcagcc cttgacgcgt cggaactatt
     1861 aaaagctcgc ccacaaccag gggcgtggcg gaagtgtttc cttcttcacc gaccggcaaa
     1921 gtgttcaatt gcttgccatc ctttagagag aggacgatgc acgcatctcc accgagcgga
     1981 gagaagagcg cgcttcgatt gtctgtcagc ggtagagccg cgatccgtcc ggccagctgc
     2041 cttttccata acagttcacc ccggcgcggc gagagcaaat agacaaaatt gtcgagagat
     2101 gtgacgatga tgccgctatc cgtcagcacg accgcttgca ccgctgctcc cgcccgacgc
     2161 cgccagagaa gccgaccatt catttcacta aaagcataga tatatccatc cgccgagccg
     2221 aagtaagtaa tattgttggc aaccgccact gcacccctta atgcgcctcg cgtccgataa
     2281 cgccagaggg ttgctcccga tgattcatcg agggcgaaca gggtgccgtc ctcgctgccg
     2341 atgtataaac gcccttggtg gagcgacggt tgggaagaga aaccgctcgt gtgttgcact
     2401 gtccacagta tttcccccgt cgctttattg aatgcgtaga tacggccatc tgccgaaccg
     2461 ccaaagagtc tcgtagcaga ggaggtgagt cctccttgta tggggaagtg aagcgtgcgc
     2521 atccagaggg taatcccggt gctctggctc aatgcgcgaa tcgcccctgt cgagcgtaat
     2581 acctggcttg aattggttgt ctcgccggct ttagacgcaa tatagacgcc gcgctcgtct
     2641 gcaatcggtg aagcagagaa ctctcctccg atctctgttc tccatctgag gtgcccgtca
     2701 gctgcattca aagagacaat ttcaccggca gccaggggta agtaaatata actattgctc
     2761 gaagcagggg agagggtaat agtattgtgt gaatcgaact gccagcgaat catgagggga
     2821 gaggaaaaaa taagcccctt ttcaaccttc gaaatagaga aaggtgcgct gtaaactttt
     2881 gtcgggaata gcaaaaacaa ggggagcagt aaatagagga gactccttga accctgatat
     2941 ttattattat ggtttcgctc gcacattttg tcttaaatgt taaaatccac ttgaagtctc
     3001 gattttatcg ctggaagcgg ggttgtttta acgcccgcat attctgtcaa actatcaatg
     3061 ttatcgacct cgcaatgctt tccggaggat gactgttaag tggaaactac gcaacacgat
     3121 ctcatactta tacttgattt tggttcccag tacacccaac tcatcgcgcg tcgaatacgc
     3181 gaagcgggag tttactgcga aattctgccc ttcaataccc ctctggaaaa aatagatttg
     3241 ctcaagccgc agggaattat tctctc
//
LOCUS       DASWNS010000336         3515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_322863, whole genome shotgun sequence.
ACCESSION   DASWNS010000336 DASWNS010000000
VERSION     DASWNS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322863"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..556)
                     /locus_tag="VGB76_11765"
     CDS             complement(200..556)
                     /locus_tag="VGB76_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099616.1"
                     /translation="MISDHEWRGVPLERVAEWRAVFQSSREGLDLSAPCPLCGVKALR
                     RWYQVNRPEEMVIDGVRYVGRGGLWEWCGDCLRYEHYSARVPEWWACDLEVDERRLTA
                     LPTAIEEAIRLRGQIR"
     gene            complement(821..1411)
                     /locus_tag="VGB76_11770"
     CDS             complement(821..1411)
                     /locus_tag="VGB76_11770"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0099617.1"
                     /translation="MSVERQDEVTRILHDWSGGDADAPARLMPFVYDELRRLARIFLA
                     RERGAHTLQPTALVHEAYLKLVDQRSVKWQNRAHFYGLAASMMRRVLIDHARAHATEK
                     RGGAAQHLSLDEVQVPLEQRAAGLLALDEALERLAQMDERKCRVVEMRFFGGLSDEEI
                     AEVLKVTTRTVLRDWKKARLWLYRELSLDQEKERES"
     gene            1741..>3515
                     /locus_tag="VGB76_11775"
     CDS             1741..>3515
                     /locus_tag="VGB76_11775"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEY0099618.1"
                     /translation="MKTTRNFTRQRLINTFLPLLLICILGLLAALFAPTLRPGAPQHS
                     AAAETKFSTPSPSSNSAQATTLTTERAREAYGRMELSFEANHGQTDASVNFLARGAGY
                     TLFLKPAEAVFVMARQGATPVRKDADRAPKTNGNAGIVPTERVTTARASKGVSGEPPL
                     TLSMKLVGADAGATVEGADELAGKVNYFKGDDPAKWRTNVPTFGRVRYKEVYPGIDVV
                     YYGNQRQLEYDFVVAPGRDARAVSLEFAGADKLEVDAGGDLLLTVGGEVVRQRKPVIY
                     QEAAGGARREVEGGYVLQPRGRVGFHVGEYDAERPLVIDPILVYSTYLGGSGTDDGND
                     IKVDSAGNAYICGNTSSTNFPTANAFQATFGGANFEGGRDGFITKLNATGSGFIYSTY
                     LGGSGDDRCNKIAVDSTGNAYVVGESISTNFPRANAFQNTYGGGLSDATITKLNANGD
                     ALVFSTYLGGSIFDAGNGIALDSSNNVYITGRTTSANFPTANAIQAAQASQSADAYVT
                     KLNAAGNALVYSTYLGGSDGTEGGFDAGFSIAVDSAGGAYVTGQTRSTNFPTVNAVQS
                     TFGGGFPDGDAFVTKINAAGNAFAYS"
BASE COUNT          709 a         1185 c          988 g          633 t
ORIGIN      
        1 agcgtgaagc gtaaatagat aaaacctgtc ttagtctatt tactagatag ataaagttat
       61 ttgaattgaa ctgtaccgag agccgctcaa cgtccggcat cagcgacgcg cacaggacat
      121 aacagaccgg aagcacgctt atgtgcgcgt ccgttgcagc cgttgttaga ttgcgccttc
      181 ccatgagatg gaaccacgcc taacgaatct gcccccgcaa tcggatcgct tcttcgatag
      241 ccgtgggtaa cgccgtcagc ctccgctcat caacttccaa gtcacacgcc caccactcag
      301 gcacgcgcgc ggagtagtgc tcgtacctga ggcagtcgcc gcaccactcc cacaacccgc
      361 cgcgcccaac atatctcacc ccgtctatca ccatttcttc aggccggttt acctgatacc
      421 agcggcgtaa tgctttgacg ccgcagagag ggcagggggc agacaggtcg agtccctcgc
      481 gcgaagactg aaaaactgcc cgccactctg ctacacgctc cagcgggacg ccacgccact
      541 catggtctga gatcataaac gttcttaacg tgaagcaatc taacgcatat tgtgccgcac
      601 tgtgcggcct aacacacccg gatacgtgtc aaaagccgct cctagttgag caggatgctt
      661 tgttagcacg ccgccattta actacacgcc acacaggtca agccggaaag aatccgactc
      721 ctgccccggc gcgcaactca aaggccaccg ctcgccgggt aagttcggct tgtcctctct
      781 gcgccaggta cgtcaactat gttattgccc tattgacgat tcacgattca cgttccttct
      841 cttgatccag tgagagttcg cggtagagcc agaggcgcgc tttcttccaa tcgcgcagga
      901 cggtgcgggt cgtgaccttg agcacttctg cgatctcttc atcgctcagg ccgccgaaaa
      961 aacgcatctc cacgacccgg cacttgcgct cgtccatctg cgccaatctc tccaacgctt
     1021 cgtcgagcgc gagcagcccg gcggcgcgtt gttcgagcgg cacctgcacc tcgtcgagcg
     1081 agaggtgctg cgccgccccg ccgcgtttct ccgtggcgtg cgcgcgcgcg tggtctatga
     1141 gtacgcgtcg catcatgctg gctgcgaggc cgtagaagtg cgcgcggttt tgccatttaa
     1201 ccgagcgttg gtcaacgagc ttgagatacg cttcgtgtac gagcgcggtc ggctgcaagg
     1261 tgtgcgcgcc gcgttcgcgg gcgaggaaaa ttcgcgccag ccgccgcagt tcgtcgtaga
     1321 caaacggcat gaggcgtgcc ggggcgtcag cgtcgccgcc gctccaatcg tgcagaatcc
     1381 gcgtgacttc gtcctgccgc tcgacgctca tcttcgttgc gccctccgcg ccgcgccgcc
     1441 gattctagcg cggaaaaact ttacccgtcc aaaattttcg cccgccgtgt caggttgcga
     1501 cgcccgctct cgcgtatacg tgcgaaaggc aaattaaaaa gattgcttga ccgggcgcgc
     1561 ggatcgtgga agtatgcttc gcctaatcca gaccgtgggt gaaagagccg ggcgttaacc
     1621 tgatatcagc cgagttgtct gctacagcgc acgccgcgca tgcccgccag agaatcaacc
     1681 tcaaagcgtg tcgccgcaca cgccttcaac caagacgagg acaacatgac atctgccccc
     1741 atgaaaacca ctcgcaactt cacccgtcaa cgcctgatca acacgttcct gccgctccta
     1801 ctgatctgca tcctcggcct gctcgccgcg ctgttcgcgc cgacgctcag accaggcgcg
     1861 ccgcaacatt ccgccgccgc cgaaacaaaa ttttcgacac cgtcgccctc atcaaactca
     1921 gcgcaggcga cgacgctcac gaccgaacgt gcgcgcgagg cttacggtcg gatggagttg
     1981 agtttcgagg ccaaccacgg gcagaccgat gcctccgtca acttcctcgc gcgcggcgcg
     2041 ggctacacgc tctttctcaa acccgcagag gccgtcttcg tcatggctcg acagggcgca
     2101 acccctgtgc gcaaagatgc cgaccgcgcg ccaaagacga acgggaacgc gggcatcgtg
     2161 ccaaccgagc gcgtaaccac tgcccgcgcc tccaaaggcg ttagcggcga gccgcccctg
     2221 acgctcagca tgaaactcgt cggcgcagac gcgggcgcaa cggtcgaggg cgcagacgag
     2281 ttggcgggca aggtcaacta tttcaaaggc gacgatccgg ctaagtggcg caccaacgtc
     2341 ccgaccttcg ggcgcgtgcg ctacaaggaa gtctatccgg gcatcgacgt agtctattac
     2401 ggcaaccagc gacagttgga atacgacttc gtggtcgcgc cgggacgcga cgcgcgcgct
     2461 gtgagccttg agttcgcagg cgcggacaag ttggaagttg atgcgggcgg cgacctgctc
     2521 ttgacggtgg gcggggaagt cgtgcggcaa cggaagcccg tcatttatca ggaagcggcg
     2581 ggcggcgcgc ggcgcgaggt agagggcggc tacgtgttgc agccgcgtgg gcgcgtcggc
     2641 ttccacgtcg gcgagtacga cgcggagcgt ccgctcgtca tcgatccgat cctggtttac
     2701 tcaacctatc tcggcggcag cggcacggat gacggcaacg acatcaaagt ggactccgcc
     2761 ggtaacgcct acatctgcgg caacacctct tcgaccaact tccccaccgc caacgccttt
     2821 caggccacct tcggcggcgc gaacttcgag ggcgggcgcg acggtttcat cacgaagctc
     2881 aacgcgacgg gttcgggctt catctactcc acctacctcg gcggcagcgg cgacgaccgc
     2941 tgcaataaga tcgcggttga ttccaccggc aacgcatacg tcgtcgggga aagcatctca
     3001 accaacttcc cccgcgccaa cgccttccag aacacctacg gtggcggcct ctcggacgcc
     3061 accatcacga agctcaacgc gaacggcgac gcgctcgttt tctctaccta cctcggcggc
     3121 agcatttttg atgcgggcaa cggcatcgcg ctcgattcct caaacaacgt ctacatcacg
     3181 ggacgcacca cctctgcgaa cttccccacg gccaacgcca ttcaggcggc gcaggccagc
     3241 caatccgccg atgcctacgt gacgaaactc aacgcggcgg gcaacgcgct cgtctactcc
     3301 acctacctcg gcggcagcga cggcaccgaa ggcgggtttg acgctggctt cagcatcgcg
     3361 gtcgattccg ccgggggcgc atacgtcacg ggacagaccc gctccaccaa cttccccacg
     3421 gtcaacgccg tccaatccac cttcggcggc ggcttccccg acggcgacgc ctttgtcacg
     3481 aagatcaacg cggcgggcaa cgccttcgcc tactc
//
LOCUS       DASWNS010000337        11257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_222147, whole genome shotgun sequence.
ACCESSION   DASWNS010000337 DASWNS010000000
VERSION     DASWNS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11257
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222147"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VGB76_11780"
     CDS             <1..672
                     /locus_tag="VGB76_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099619.1"
                     /translation="TRVANPRTQGRLVLLVVVALMGLAIVFDWTSANSAHAEVTAELK
                     RQQEQAARMEAIKKEQAELEKKTQEIQTRIDAIKKLRASQQGPVAVLSAINERLPKIT
                     DFRLESIEQKGTELVLKGDSPNEAAVTQFGRSMEFSSGLFTNVSIETKRELMEKVNVG
                     STPPAGATDEASKPLVKPDTVTFTLKCKYTQPGSQTSSAPATGSNAKPSGANAAPAPP
                     QVAKK"
     gene            720..1367
                     /gene="pilO"
                     /locus_tag="VGB76_11785"
     CDS             720..1367
                     /gene="pilO"
                     /locus_tag="VGB76_11785"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY0099620.1"
                     /translation="MLKLDSLQQKPWYFQLAIFGFVAFVIYGGFWYFVTSGTREETKT
                     LQAQVDKLQQENARAQIASQRLNDFKATYARAQADYEDLKALLPEQRELTTVLQSLQE
                     RARGRLSVTRFTPKDDIQQDFYAGKPVEIEVASSYNNLGAFFAQIAAYQRIVSISDFK
                     LSRMKEQGAGKTVEAQFLLTAYYASSEKLQNTAKPAGAPTVPGAAPTVPAVPAAK"
     gene            1434..2060
                     /locus_tag="VGB76_11790"
     CDS             1434..2060
                     /locus_tag="VGB76_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099621.1"
                     /translation="MNYTKMIKQTLQATLAAGALLTFSATAFGQEASTTYRGAGRDPF
                     TKYKAPVKRVKPVPQPVPAPSIEQRIAAYKTQKAIAMNAQLPAPKPTTALLLSEAQVT
                     GIFRTPRGYAAMVEAKPIKLSYVIYPGETFFDGMLVAIEEDRLVFRKETRWSDGRREM
                     NVEMKALGATNPVKDTLTNTNPAPTAATTNGTASPKAKEESAPAQAKQ"
     gene            2335..4521
                     /gene="pilQ"
                     /locus_tag="VGB76_11795"
     CDS             2335..4521
                     /gene="pilQ"
                     /locus_tag="VGB76_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEY0099622.1"
                     /translation="MVNLHHPLSAVIKRGALVLFLLCSISTFTHAATSRLLTGVRTSV
                     DGDATRITFAGTAPMTYGVRRVDERTLMVELPGVDATRLAPSYSPASPLVAGLTVQCA
                     TGSDGAPFARLRVALRASARDRSFLDTNELVLELRPEDSPLQQQQPQQRVAREAHAVV
                     VTGAEQQTTTGRPSPTPSISAAQGAGQQSSQGQQYGQYGFVGEPINLNVVNADIRDIL
                     NYITEQYGVNFIIDSSVGAVPVTVNVTEVPWNIALNSILRANRLGIEVSGNILRVATL
                     KVLAEEAEAQGSIEEARLNAAPLVTEFIRLNYARAVGTLAQAAGSTGGFSGGASNESF
                     SGGSGGDATGKSGDKGILPIISRRLSKRGSVEVDGRSNTLIITDVVENIASIRQLVSL
                     LDQPEPQVEIETRIVVASRSFSRDLGVQLQAAVFGSNGQGGSFETAPANGGLRPGGLP
                     GGLSNTPPSTGGLGATAPTTVIGLTTGIFGTAQIGALITAAESKGQAKIVANPRVTAL
                     NNRPAQIESGAQIPVVTPQAAGAGGSGDNLGGSTVFTTTFISVPLRLSITPQITDAGT
                     VVLRVTAENNSVNRSLTNSLGTPGIDTQRMQTEVLVPDGGTTVVGGVLLDSEGETQTR
                     TPGISGIPVLGNLFKRKATTRNTNELLFFITPRIYRPDYNGRPMAGTITDRNRTTTIP
                     QPVPLGNPQSNTPAPSVEGQQAQPNSQTQSDTAPTARPAATRGERP"
     gene            4546..4911
                     /locus_tag="VGB76_11800"
     CDS             4546..4911
                     /locus_tag="VGB76_11800"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEY0099623.1"
                     /translation="MFKETLTRMVERTEGAVGALIMGTDGIAVEKVLMPEGRDANLDV
                     AAAEFTSLLRNAQRAGGDIGLGQVREVVVGFDDMNVLMRLFNPEYFVLLALTSDGNIG
                     RGRFELRKVELDLAREFAV"
     gene            complement(5010..5777)
                     /locus_tag="VGB76_11805"
     CDS             complement(5010..5777)
                     /locus_tag="VGB76_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEY0099624.1"
                     /translation="MIRRIWKLIKAVVIGVLAFIVLAMIYEIATFPRVARLRDNNPET
                     TAMIETRMQEARERGEQPKRVQQWVPLERISVHLLRAVLAGEDSNFATHNGFDYDAIQ
                     KAWDQAQRDAAKEAKQQGEDESSWIPAMPSFKRGASTISQQLAKNLYLTTERSFVRKA
                     REAAITYFMEKNLSKRRILEIYLNVIEWGDGIYGAEAAARTYFRKSAADLTPQEAAFL
                     SAIIPNPRTEFNPQLKPKRVARRQRIIARGMPHVRLP"
     gene            complement(5788..6717)
                     /locus_tag="VGB76_11810"
     CDS             complement(5788..6717)
                     /locus_tag="VGB76_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY0099625.1"
                     /translation="MHVFYTPRYYADIGEGHVFPIRKFELVRDRLLAEGTLAPSEIIE
                     PQPAPVADVLLVHTEDYVTRLRAGSLTPRELRRLGLPWSKALVRRSFLATGGTLAAAR
                     AALDVGIASNLAGGTHHAFPDHGEGFCVLNDVAVAIRALRRDGAIERVAVVDCDVHQG
                     NGTATIFAGDASVYTFSVHGAKNYPVFKARSTLDIELPDGTGDEEYLRTLAEHLPRVF
                     AHRPELLFYLGGADPFAGDKLGRLSLSIAGLRARDEFVLSECSARGVPVATVMSGGYA
                     ADINDTVEIHCNTIRAAKALEVARSMKRNSVRL"
     gene            complement(6850..7683)
                     /locus_tag="VGB76_11815"
     CDS             complement(6850..7683)
                     /locus_tag="VGB76_11815"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0099626.1"
                     /translation="MKRFSIFLRRIRAFIARDFQLEISYRFAFFLRVLSVLALVTTLY
                     FVSRTFLAPGDCPCAASPFAQWRDPMAAWITGLAVLNYFMTGFSSLANAIRSEQTQGT
                     LESVLLTPINIPTLVASSSAWAFVQATFFSFLYFFFGWVFFNVHFQGNFLLAFFILLL
                     TTLVLSSLGILSASFAMVFKRGDPFGILLGTGSALFSGVFFPTQILGSGLSTISRVLP
                     PTYGLDGIRRVLIEGQGFNEVRVPIFMLLLFLAVLMPFSLWVFSRAVRRAKREGSLIQ
                     Y"
     gene            complement(7680..8768)
                     /locus_tag="VGB76_11820"
     CDS             complement(7680..8768)
                     /locus_tag="VGB76_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0099627.1"
                     /translation="MTATTPLLYQDHTEDTARDTPTQSIAISIRQLSKTYPVPLARLK
                     KFLRRKATNPVEALRDVSFDVREGEIFGLIGRNGAGKTTLTKIIATLVQPTSGAVVVR
                     GFDSVRDEETVRAQIGLATAEERSFYWRLTIEQNLNFFARLYGLTDASARARINELLT
                     RLGLESLARRRFGELSTGNKQRVAIARAMLSSPHILLLDEPTRSLDPLAAAQTRSLIN
                     SLARDADKPVTVLLTSHNLAEIEELCPRVAIISRGEIRALDTPEHLRALHRSAERVRL
                     SVGSLSPESARRALAATLDEFELSLDAGKLSVQFTRAVDDDALDRTIRALQTAGGRIL
                     SSEAERATLLDVLEKYEQEHEAAGGEQR"
     gene            complement(8798..9613)
                     /locus_tag="VGB76_11825"
     CDS             complement(8798..9613)
                     /locus_tag="VGB76_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0099628.1"
                     /translation="MNRLSKFGLVVVGVFVIMAVFAPVIATHDVGATDLLNRYMPPSS
                     AHWLGTDATGRDVFSRVIYGARISLRVGLVVVFVSTVVGTVIGALAGYYGGWIDRIVN
                     GYLFNVFLAFPGLLLAIAMVAFLGAGLNKLILALCIIGWVGYARVIRGQVLKVREYDF
                     VQAARALGASDMRILFVHILPNAIQPLIVQASLGMAGAVLAEATLSFLGLGVPPPAPS
                     WGVMLEEARDLSTLQAAPHALVVPGIAIALSVLAFNFIGDGLREYLDPRQRKR"
     gene            complement(9699..10694)
                     /locus_tag="VGB76_11830"
     CDS             complement(9699..10694)
                     /locus_tag="VGB76_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0099629.1"
                     /translation="MKHRLLDLSRRILILLLVVWTVVSLVTLLIELVPGDPATATLGE
                     DATPAQIEQFRQKHNLDRPAFFFSYARDAASGERDFAWHGLNNRYIDYWGTLLSGDMG
                     RSFRTDRPVIELIMQRYPATIQLAVAALLVAVSIALPLGVIAGKNRGTWLDNLLSVIA
                     LVGVSMPGFVLGPLLIYLFALQLGWLAPSGRFEWYDIILPAVTLGAALSALLTRMVRS
                     SVIEEMGEDYVRTARAKGLNERTVVYKHVLKNGLIPVVTVLGLQLGVLLAGAIITEKI
                     FNWPGLGLLLIDDGIGKRDYRLVQGCVLVISVTYIFANTLTDIIYRWLDPRIRIQ"
     gene            complement(11002..>11257)
                     /locus_tag="VGB76_11835"
     CDS             complement(11002..>11257)
                     /locus_tag="VGB76_11835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0099630.1"
                     /translation="PIVPGFRALFQFYFSRVLPRIGGLVSGSRGAYEYLPDSVSRFPD
                     QKRLAELMREAGFAEVSFQNLTGGIAALHLGTRPRQRRSS"
BASE COUNT         2406 a         3491 c         3456 g         1904 t
ORIGIN      
        1 acgcgcgtcg ccaacccgcg cacgcagggc agactcgtgc tgctggtcgt cgtggcgttg
       61 atggggctgg cgatagtctt cgattggacg agcgcgaatt cggcgcacgc ggaagtgacg
      121 gcggaactca agcgacagca agagcaggcc gcgcgcatgg aagcgatcaa gaaagagcag
      181 gccgaacttg agaagaagac gcaggaaatt cagacgcgca tcgacgcgat caaaaaattg
      241 cgcgcttcac aacagggacc ggtcgcggtg ctctcggcca tcaacgagcg tttgcccaag
      301 attacggatt tccgtctgga aagcatcgag cagaagggca cggagttggt cttgaaaggc
      361 gactcgccga acgaagccgc tgtcacgcag ttcgggcgca gcatggaatt ctcgtccggc
      421 ctgttcacca acgtcagcat cgagacgaaa cgcgagttga tggaaaaagt gaacgtgggt
      481 tcgacgccgc ctgcgggcgc gacggacgag gccagcaaac ctctcgtcaa accggacacc
      541 gtgaccttca ccttgaagtg caagtacacg cagccgggta gccaaacgtc ttccgcgccc
      601 gccaccggca gcaatgcgaa gccgtccggc gcaaacgccg ctcccgcgcc ccctcaagtc
      661 gcaaaaaaat aggcactgtc gagagtttcg accgcgcgcc cgaagggaac tgacatctca
      721 tgttgaaatt agattcatta caacagaaac cctggtactt ccagctcgcc atctttggct
      781 ttgtcgcgtt cgtcatctac ggcggcttct ggtatttcgt cacgagcggc acgcgcgaag
      841 agacgaagac gctccaggcg caggtcgata agctgcaaca ggagaacgca cgcgcgcaga
      901 tcgcctcgca gcgactcaac gacttcaagg ccacctacgc acgcgcgcag gccgactatg
      961 aagacttgaa ggcgctcctg ccggagcagc gcgaactcac gaccgtcctg caaagtttgc
     1021 aggagcgtgc gcgcggtcgc ctctccgtga cgcgcttcac gccgaaggac gatatccagc
     1081 aggacttcta cgcgggcaag ccggtcgaga tcgaagtcgc cagttcgtat aacaacctcg
     1141 gcgcgttctt cgcgcagatc gccgcctatc aacgcatcgt ctcgatcagt gatttcaaac
     1201 tttcgcgcat gaaagagcag ggcgcgggca agaccgtcga agcgcagttt ctgttgacgg
     1261 cctactatgc ttcgagcgag aagttgcaga acactgcgaa gcctgccggt gcgccaaccg
     1321 tgccgggagc cgctcccacc gttcccgccg tccccgccgc gaagtagtta cacgactcca
     1381 tcgccgcgag aaaacgcggc cagaagaagc tgacccactc aggaataaaa gagatgaact
     1441 acaccaagat gatcaagcag accttgcagg ccacgctggc cgccggcgcg ctgttgacat
     1501 tctccgcaac ggccttcggg caggaagcga gcaccactta caggggcgcg gggcgcgacc
     1561 cgttcacgaa gtacaaagcg cccgtcaaac gtgtgaagcc cgtgccgcag cctgtgcccg
     1621 cgccttccat cgagcagcgc atcgccgcct acaagacgca aaaggccatc gcgatgaacg
     1681 cccagcttcc cgctccgaag ccgaccaccg cgctgctcct gagcgaagcg caggtgaccg
     1741 gcatcttccg caccccgcgc ggatacgcgg cgatggttga agccaaaccg atcaaactct
     1801 cttacgtcat ctatcccggc gaaactttct tcgacgggat gttagtcgcc atcgaagaag
     1861 accgcctcgt gtttcgtaaa gagacgcgct ggagcgacgg gcggcgcgag atgaacgtcg
     1921 agatgaaagc cctcggcgcg accaaccccg tcaaagatac tttgacgaac accaacccgg
     1981 ctccgacagc cgccaccacc aacggcaccg cctcgcccaa agctaaagaa gagagcgcgc
     2041 ctgctcaagc aaaacagtga cgctcttctt ttcgttcgtt ccgcgcgccc acggaagcct
     2101 gagccgccgg gcgcgcttga actaaacctt ctcccgtcgc acacccgcga gagatttccc
     2161 ggcgcagacg gcgcatgcaa tctgctgcgg actgtgctcc cgctatttgg caagacaaat
     2221 tcgccgtcgg ctttcgcatt gtgcggcgcg cgcttgaaga gtcctcatcc gactcgcacg
     2281 agcgttagcg cgtggcgtta aagttgacgg tcactcctga caaaggaaaa gaaaatggtt
     2341 aacctgcatc acccgctttc agcggtcata aaaagaggcg cgctcgtcct cttcctttta
     2401 tgctcaatct caactttcac gcacgcggca acttcacgtc tgctgacggg cgtgcgtacg
     2461 tccgtcgatg gcgacgcgac gcggatcacc ttcgccggaa ctgcaccaat gacctacggc
     2521 gtgcgccgcg tagacgaacg cacgttgatg gtcgaactgc cgggcgttga cgccacgcgg
     2581 ctcgcgccct catattcacc ggcctcgccg ctcgtcgccg gactcacagt gcaatgcgca
     2641 accggcagcg acggcgcgcc ttttgcgcgt ctgcgcgtcg cgctccgcgc ctcggcacgc
     2701 gaccgctcct tcctcgacac caacgaactc gtcctcgaat tgcgacccga agatagccct
     2761 ctgcaacagc aacaaccgca acagcgcgtc gcgcgtgaag cgcatgccgt cgtggtcacc
     2821 ggcgcagagc agcagacgac caccggcagg ccgtcgccta cgccgtccat cagcgcggcg
     2881 cagggcgcgg gtcagcaatc ttcgcagggt caacagtacg gccagtatgg tttcgtcggc
     2941 gagccgatca acctgaacgt cgtcaacgcc gacatccgcg acattctcaa ctacatcacc
     3001 gaacagtacg gcgtaaactt catcattgat tcttccgtcg gcgcagtgcc cgtcacggtc
     3061 aacgtcacag aggttccctg gaacatcgcg ctcaactcga ttctgcgtgc gaaccgtcta
     3121 gggatcgaag tgagcggcaa cattctgcgc gtcgcgacgc tcaaggtgct ggccgaagag
     3181 gccgaggcgc aaggctcgat tgaagaagcg cgcctgaacg ccgcgccgct cgttacggaa
     3241 ttcatccgcc tcaattacgc acgcgcggtc ggcacgctgg cacaggccgc aggctccacc
     3301 gggggcttct ccggcggcgc gagcaacgag agcttctccg gcggctcggg cggcgacgcc
     3361 acgggcaaga gcggcgacaa aggcatcctc ccgatcatct cgcgtcgtct ctccaagcgc
     3421 ggcagcgtcg aagttgacgg tcgcagcaac acgctcatca tcacggacgt ggtggaaaac
     3481 atcgcctcca tacgccaact cgtctcgctc ttagatcaac ccgaaccgca agtcgagatc
     3541 gagacgcgca tcgtcgtcgc cagccgcagt ttcagccgcg acctcggcgt gcagttgcag
     3601 gctgcggtct tcgggtcgaa cgggcagggc ggctcttttg agacggctcc cgccaacggc
     3661 ggcctgcgtc cgggcggact tcccggcggc ctcagcaaca ctcccccttc gacgggcgga
     3721 ctcggcgcga ccgcgccaac cactgttatc ggcttaacca caggcatctt cggcacggcg
     3781 caaatcggcg cgctcatcac cgccgcggag agcaagggtc aggcgaagat cgtcgccaac
     3841 ccgcgcgtga ccgccttgaa caatcgcccc gcgcagatcg aaagcggcgc gcagattccc
     3901 gtcgtcacac cgcaagcggc gggtgcgggc ggctcgggcg acaacctcgg cggctcgacc
     3961 gtcttcacca ccaccttcat ctccgtgcct ctgcgtctct ccatcactcc gcagatcacg
     4021 gacgccggaa cggtcgtcct acgcgtcacg gcggagaaca acagcgtcaa ccgctctctg
     4081 accaactcgc tcggcacgcc cggcatcgac acgcagcgca tgcagaccga agtgctcgtg
     4141 cccgatggcg gcaccacggt ggtcggcggc gtgctcctcg atagtgaagg cgagacgcag
     4201 acgcgcacgc ccggcatctc cggcatcccc gtcctcggca atctcttcaa gcgcaaggcc
     4261 accacgcgca acacgaacga actgctcttc tttatcacgc cgcgcatcta tcgccccgac
     4321 tacaacgggc gtccgatggc cggaaccatc accgaccgca accgcacgac gaccatcccg
     4381 cagcccgttc cgctcggcaa cccgcagagc aacacgcccg cgccgtcggt cgaaggccaa
     4441 caggcgcagc cgaactcgca gacccagtct gacaccgctc cgactgcgcg ccccgccgcc
     4501 acacgcggcg aacgacctta agaggaagga gacaaagaga gtctcatgtt taaggaaaca
     4561 ctcacacgca tggttgaacg cacggagggc gcagtcggcg cgctcatcat gggcacggac
     4621 ggcatcgccg tcgagaaggt cttgatgccc gaagggcgcg acgccaacct cgacgtggcc
     4681 gctgccgaat tcacctcgct cctgcgcaac gcacagcgcg cgggcggtga catcggcctc
     4741 ggtcaggtgc gcgaagtcgt cgtcggcttc gacgacatga acgtcctgat gcgtctgttc
     4801 aacccggaat acttcgtcct cctcgcgctc acttccgacg gcaatatcgg gcgcggcagg
     4861 ttcgaactac gcaaagtcga actcgacctc gcacgcgagt tcgccgttta acccaggcag
     4921 agaaagcagc aagacgaagg gcgaaagcga gacggaaaca ccgtcttcac tttcgccctt
     4981 cgtcttgcta ttcgccccgc cgcttttcgt cagggcagcc tgacgtgggg catgccgcgc
     5041 gcgatgatgc gctggcggcg cgcgacgcgc ttcggtttga gttgcgggtt gaattcggtg
     5101 cgcgggttgg gaatgatggc ggagagaaag gccgcttcct gcggggtgag gtctgcggca
     5161 gatttacgaa aataggttcg cgcggcggcc tccgcgccgt aaatgccgtc gccccactcg
     5221 atcacgttga gatagatttc cagaatccga cgcttcgata aattcttctc catgaaataa
     5281 gtgatcgccg cctcgcgcgc tttgcgcacg aacgaacgct cggtggtgag ataaaggttc
     5341 ttggctaact gctgcgaaat ggtggatgcg ccgcgcttga aactcggcat cgccggaatc
     5401 caactcgact cgtcttcgcc ctgctgcttc gcttccttcg cggcgtcgcg ctgcgcctga
     5461 tcccaagcct tctggatagc gtcatagtcg aatccgttgt gcgtggcgaa gttgctgtct
     5521 tcgcccgcga gcacggcgcg cagcagatgg acggagatgc gttccagcgg aacccattgc
     5581 tgcacgcgct tcggctgttc gccgcgttcg cgcgcctcct gcatgcgcgt ctcgatcatg
     5641 gcggtcgtct ccgggttgtt gtcgcgcaat ctcgccacgc gcggaaacgt cgcgatctcg
     5701 tagatcatcg cgagcacgat gaaggcgagc accccgatca caaccgcttt aatcaacttc
     5761 cagatgcgtc ttatcatggc ctacgaatta tagccgaact gaattccgct tcatacttcg
     5821 agccacctcc agagctttgg ccgcgcggat cgtgttgcag tgaatctcca ccgtatcgtt
     5881 gatgtctgcc gcgtaaccgc ctgacatcac ggtcgccacg ggcacgccgc gcgcgctgca
     5941 ttcggacagg acaaactcgt cgcgtgcgcg caagcctgcg attgaaagtg agaggcgtcc
     6001 gagtttatcg cccgcaaaag ggtctgcgcc gccgagataa aagaggagtt cggggcggtg
     6061 cgcgaagacg cgcggcaagt gttcggccag cgtgcgcagg tattcttcgt cgcccgtccc
     6121 gtccggcagt tcgatgtcga gcgtcgagcg ggctttgaag acgggatagt tcttcgcgcc
     6181 gtgcacggag aaagtataga cgctcgcgtc gcctgcaaaa attgtggccg tgccgttgcc
     6241 ctgatgcacg tcgcaatcaa cgaccgccac gcgctcgatt gctccgtcac ggcgcagggc
     6301 gcgaatcgcg acggcgacat cgttcagcac gcagaagcct tcgccatgat cgggaaaggc
     6361 gtgatgcgtg ccgcccgcga ggttggacgc gatgcccaca tcgagtgcgg cgcgtgcggc
     6421 ggcgagcgtg ccgccggtcg caagaaacga acgtcgcacg agcgctttcg accacggcag
     6481 accgagcctt cgcaactcgc gcggcgtgag cgaaccggcg cgcaggcgcg tgacgtaatc
     6541 ctccgtgtga accagcaaca cgtcggccac gggcgcgggc tgcggttcga tgatctctga
     6601 cggcgcgagc gtgccttcgg cgagcaggcg gtcgcgcacg agttcaaatt tgcgaatcgg
     6661 aaagacgtgc ccctcgccga tgtccgcgta ataacgtggc gtgtagaaaa cgtgcataga
     6721 tgccgtcggc tatcagctat gagcctttag tcaaacttac ctttgtcatg tagccctcac
     6781 ggtaatgtag acctcacgct ctcaacgcat caactcgtcc ggcaattgtt caaagttaac
     6841 agctcacggc taatactgaa tcaggctgcc ttcgcgtttg gcgcggcgga cggcgcggga
     6901 gaagacccag agcgagaagg gcatgaggac ggcgagaaac aggaggagca tgaagatcgg
     6961 aacacgtacc tcgttgaaac cctgcccctc gatcaagacg cgacgaatgc cgtcgagtcc
     7021 gtaggtcgga ggcagcacgc gcgagatcgt gcttaagccg ctgccgagta tctgcgtggg
     7081 aaaaaagacg ccggagaaga gagccgaccc ggtgccgagc agaatcccga agggatcgcc
     7141 gcgtttgaag accatcgcaa aactggcgga gagaatgccg agcgaagaga gcacgagggt
     7201 tgttagcaga agaataaaaa acgcgagcag gaagttgccc tgaaaatgga cgttgaagaa
     7261 tacccagccg aaaaagaaat agaggaagga gaagaaggtc gcctgcacga aagcccatgc
     7321 cgaactcgaa gccacgagcg tcgggatgtt gatcggcgtc agcaacacgc tttcgagcgt
     7381 cccttgcgtc tgctccgaac gaatggcgtt agcgaggctg gagaagccgg tcataaagta
     7441 gttcaagacc gccagacccg tgatccatgc ggccatcggg tcgcgccatt gcgcaaacgg
     7501 cgacgcggcg caggggcaat caccgggcgc gagaaacgta cgagagacga agtagagcgt
     7561 cgtcacgagc gcgaggacgc tgaggacgcg cagaaaaaag gcgaagcgat aactgatctc
     7621 caactgaaaa tcgcgcgcga tgaaagcgcg tatacgacgc agaaatatgc tgaatctctt
     7681 cacctttgct ccccccccgc cgcctcgtgc tcctgttcgt atttttcgag cacgtcgagc
     7741 agcgtggcgc gttcggcttc gctcgaaagg atgcgcccgc ccgcagtttg gagcgcgcga
     7801 atcgtgcggt cgagcgcgtc gtcatcgacg gcgcgggtga attgcacgct gagttttccg
     7861 gcgtctaacg aaagctcaaa ctcatccaac gtcgcggcca gcgcgcgacg cgccgactcc
     7921 ggcgagagac tgccgacgct caaacgcacg cgctccgcgc ttctatgcaa cgcgcgcaag
     7981 tgttcgggcg tgtcgagcgc gcgaatctcg ccgcgcgaga tgatggcgac gcgcgggcac
     8041 agttcctcga tctcggcgag gttgtgcgag gtgagaagca cggtcacggg cttgtctgcg
     8101 tcgcgcgcga gcgaattgat gagcgaacgc gtctgcgcgg cggcgagcgg gtcgagcgaa
     8161 cgcgtcggct cgtcgagcag gaggatgtgc ggcgaactga gcatcgcgcg ggcgatggcg
     8221 acgcgctgct tgttgcccgt cgagagttcg ccgaaacggc gccgggcgag cgattcgagg
     8281 ccgaggcgcg tgagcaactc attgatgcgc gcacgcgccg acgcatccgt gaggccgtag
     8341 aggcgcgcga agaagttgag attttgttcg atggtcagac gccagtagaa actgcgttct
     8401 tcggcggtgg cgagtccgat ctgtgcgcgc acagtctctt catcgcgcac gctgtcgaag
     8461 ccgcgcacca caaccgcgcc ggaggtcggc tgcacgagcg tcgcgatgat cttcgtgagc
     8521 gtggtcttgc ccgcgccgtt gcgcccgatg aggccgaaga tttcgccttc gcgcacgtcg
     8581 aagctgacat cgcgcagagc ctcgaccggg ttcgtcgcct tgcggcggag aaacttcttg
     8641 agccgcgcga gcgggacggg ataagtcttg gaaagttgac gaatagatat cgcgatgctc
     8701 tgtgtcggag tgtcgcgcgc ggtatcttca gtatggtctt gataaagaag tggagttgtc
     8761 gctgtcacag ttgagaatgc ttttcgttgg tcggttctta gcgtttgcgt tggcgcgggt
     8821 cgagatattc gcgcaggccg tcgccgatga agttgaaggc gagcacggag agcgcaatgg
     8881 cgatgccggg cacgacgagc gcgtgaggcg cggcttgcag cgtcgagaga tcgcgcgcct
     8941 cttcgagcat cacgccccag gagggcgcgg gcggcggcac gcccagacct aaaaatgaga
     9001 gcgtcgcttc ggcgagcacc gcgcccgcca tgccgaggct cgcctgcacg atgagcggtt
     9061 gaatcgcgtt aggcaaaata tgtacgaaga ggatgcgcat gtcgctcgcg ccgagcgcac
     9121 gcgccgcctg cacgaaatca tattcgcgca ccttcaacac ctgcccgcga atcacgcgcg
     9181 cgtagccaac ccagccgatg atgcagaggg cgaggatcaa cttgttcagc cccgcgccga
     9241 gaaaggccac catcgcgatg gcgagcagga ggccggggaa ggcgagaaag acgttgaaga
     9301 gataaccgtt aacgatgcgg tcgatccagc cgccgtagta gcccgcgagc gcgccgatta
     9361 cagtcccgac cacggttgag acgaagacca ccacgaggcc gacgcgcagc gagatgcgcg
     9421 cgccgtagat gacgcgcgaa aaaacgtcgc gccccgtcgc atccgtcccg agccagtgtg
     9481 ccgaagaggg aggcatgtag cgattgagca gatcggtcgc gccgacatcg tgcgtcgcaa
     9541 tcaccggcgc aaacaccgcc atgatgacga agaccccgac gacgacaagc ccgaatttgg
     9601 aaaggcgatt catttaacta cagtgacaag tggcgagtga tgagtgacga gcaaagacag
     9661 gttttagttt taacttgtcg ctcgtcaccc gtcactcgtc actgtattcg tattctggga
     9721 tcgagccagc gataaataat atccgtaagc gtgttggcga agatgtaggt cacgctgatg
     9781 acgaggacgc agccctgcac gaggcggtag tcgcgtttgc cgatgccgtc gtcgatgagc
     9841 agcagaccta agcccggcca gttgaaaatc ttctccgtga tgatcgctcc ggccagcagg
     9901 acgccgagtt gcaggccgag gacggtgacc accggaatga gtccgttttt gagcacgtgc
     9961 ttgtagacga ccgtgcgttc gttcaaacct ttggcgcgcg ccgtgcgcac gtaatcttcg
    10021 cccatctctt cgatgacgga cgaacgcacc atgcgcgtca ggagcgcgga gagcgccgcg
    10081 ccgagcgtca cggcgggcag aataatgtcg taccactcga agcgtcccga aggcgcgagc
    10141 cagccgagtt ggagtgcgaa gagatagatg agcagaggcc ctaagacgaa gccgggcatg
    10201 ctgacgccga ctaaggcgat cacggacaac aggttgtcga gccacgtgcc gcgatttttt
    10261 cccgcgatga cgccgagcgg gagcgcgatg ctgacggcga cgagcagcgc ggcgacggcg
    10321 agttggatcg tcgcagggta acgctgcatg atgagttcga tgacggggcg gtcggtgcga
    10381 aacgagcgtc ccatgtcgcc gctcaggagc gtgccccagt agtcaatata gcgattgttc
    10441 aggccgtgcc aggcgaagtc tcgctcgccg ctggcggcgt cgcgcgcgta ggagaagaag
    10501 aaggcggggc ggtcgagatt atgcttctgg cgaaactgct cgatctgcgc gggtgtggcg
    10561 tcttcgccga gcgtggccgt cgccggatcg ccggggacga gttcgatgag cagtgtgacg
    10621 agagacacga ccgtccacac cacgagaagg aggatcaaga tacgccgcga gagatcgagt
    10681 agcctgtgct tcatctacta aggatgcgcc tgcaaatatt cggaagagga atcggtttga
    10741 aactacgcgc agaatgatag cacacaaaaa cagtgacgag tgacgggtga caggtgacaa
    10801 gttaaggcag aagaaaaact ctctttatct tgtcgcccgt cacccgtcac tcgtcactgc
    10861 atataaaaaa cgtagcccga cggagtttgt gtctttcgtt acgcgaacag ccccgacaac
    10921 cgtcgcgcga gacagacact aatccatcgg actacgtttt cggtttgcgc tcatacgatg
    10981 agagcgcaag ccaatctcac gttaactgct ccggcgttgg cgggggcgcg tgcccaggtg
    11041 caaggccgcg atgccgcccg tcaagttctg aaacgatacc tctgcaaagc ccgcttcgcg
    11101 catcaactcc gccagacgct tctgatccgg aaagcgcgat acggaatcgg gcagatattc
    11161 atacgcgccg cgcgaaccac tcacgaggcc gccgatacgc ggcagcacac gcgaaaagta
    11221 gaactgaaaa agagcgcgaa accccggcac gatgggg
//
LOCUS       DASWNS010000338         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_453016, whole genome shotgun sequence.
ACCESSION   DASWNS010000338 DASWNS010000000
VERSION     DASWNS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453016"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGB76_11840"
     CDS             <1..192
                     /locus_tag="VGB76_11840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0099631.1"
                     /translation="VVELMHTEVPEEFEGQGIGGRLVKGTFELLQAENLRMMPTCQFV
                     AVYLQRHPEYQSLAVSASE"
     gene            complement(296..2815)
                     /locus_tag="VGB76_11845"
     CDS             complement(296..2815)
                     /locus_tag="VGB76_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0099632.1"
                     /translation="MNRERFHQPFLRLIPALGAVVSLYCVYRLPFDRLDLRFLILTAV
                     TLCFGSRLGIEFSKHKIQITVSDSFIFLTLLLYGTETAVLLAAVEAFCSSFRFAKLWL
                     TRFFNAGLLAISTFVTAGVVGYAIGPLVELGRETLSGRFITAVCLMCCVQFVVNSGIP
                     ALRQSMKLNLPFLQVWKEHYLWTSITYFAGASAAGVTARLVNGHGFYTLMVMIPIIAV
                     IYFTYQSYRKQLQATLAQAEQAERHAEEQRAVSQQLRQSEEQFRSSFDNAAVGMALVA
                     TDGTWIEVNPSLCAMLGYAEEELIKTDIQSVLHPEELGEHLAQVYRLTQGGAISYNLE
                     MRLLHREGRTLWAQLCASLVRDPKGQPLRLVYQVQDVTERRHAEERLQHAAHHDSLTG
                     LVNRTLFADHLRLAAERAQRHGSHLFAVLFMDLDRFKNVNDSLGHAHGDQLLLTVAER
                     LKTCIRPGDTVARFGGDEFAILLNGIRHSTDAVRAAERIQEEIALPFVLGHHGIVTSA
                     SIGITLSSLGYKNVDDMLRDADTAMYRAKSKGKGRYEVFDLTMHARAVTTLRLESELR
                     RAVEEGEFRLHYQPIVRTETGYAAGFEALIRWEHPERGIVPPGEFIAAAEETDLIIPM
                     GEWVLREACRQARAWQREFPSEEPLFVSVNLSSKQFTQRDLVEVVERALVETGLEARC
                     LKLEITETAMMENAEAATLMLKRLRELGVQLGIDDFGTGYSSLSYLHRFPVNTLKVDR
                     SFVGRMDEASEYREIVRTIVSLAHTLGMEVVAEGVETRGQRSHLNALRCDYSQGYFFS
                     KPLPAAAATEYLKNNRTRAGESLRAAPEMPGAVELVESYPM"
     gene            complement(3119..3937)
                     /locus_tag="VGB76_11850"
     CDS             complement(3119..3937)
                     /locus_tag="VGB76_11850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0099633.1"
                     /translation="MEPLLNEDRDEALAFLARRPRHTVIMAGLLRHYGPVVPLAQGMF
                     YTCRDARGRLEGLALVGRATMFEAQNGAAISALARGARRSPAVRMIMGEADELETFWR
                     CYIAGRQEPRLFCREIFYESSRQFSRAAGVDGLRQATLDDLDQIVQAHAQMVFEESGV
                     NPLADDAEGFRHRCAQRVEQGQVWTLIDKGELIFKADVLTQTPEASYIEGVWVNPAHR
                     LKGYGRNCWASLSRTLLDKAPAFCWFVNAQNSTARSFYDRVGAVSLGEYNKVYL"
     gene            complement(4181..>4426)
                     /locus_tag="VGB76_11855"
     CDS             complement(4181..>4426)
                     /locus_tag="VGB76_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099634.1"
                     /translation="GIMISDGLRMGGGSLFINGIMISDGIMISDGFALADGIMISDGI
                     MISDGIMISDSFWALNVLINGDNTNGMSVVYEDGSEP"
BASE COUNT          878 a         1259 c         1405 g          884 t
ORIGIN      
        1 gtggtggagt tgatgcatac ggaagtgccg gaagagttcg aggggcaggg aatcggcggc
       61 aggttggtca aagggacgtt tgaactactt caggcagaga acctccgcat gatgccgacg
      121 tgtcaattcg tcgccgttta cctgcaacgc catccagagt atcaatcgct agcggtttca
      181 gcttctgaat agaacctgag agagcatctg cgaaagaagc tgcggctaga gaagtgtaga
      241 gtatctgtcg cggcaaacag aacagcccgg aatggttctt cctgtcgaga gatgtttaca
      301 tgggatagct ttcaaccagt tccaccgcgc ccggcatttc cggggctgcc ctgagagact
      361 ccccggcacg cgtgcggttg ttcttgaggt attctgtcgc ggctgcggcg ggcagcggct
      421 ttgagaagaa gtagccctga ctgtaatcgc aacgcaatgc attgagatgt gaacgctgac
      481 cccgcgtctc tacgccctcg gcgacgacct ccatgccgag cgtgtgcgcc agagaaacga
      541 tggtgcggac aatctcccgg tactcgctcg cctcgtccat ccggcctaca aatgacctgt
      601 ccactttcag cgtgttgacg gggaaccggt gcaggtagga gagcgacgag tagccggtgc
      661 cgaagtcgtc tatgccaagc tggacgccga gttcgcgcaa ccgcttgagc atcaatgtcg
      721 ccgcctcggc attctccatc attgccgtct cggtgatctc cagcttcagg cagcgcgcct
      781 caagccccgt ctcgacgagc gcacgttcga ccacctctac gaggtcgcgc tgcgtgaact
      841 gtttgctcga caggttgaca ctcacgaaga gaggctcttc cgaagggaat tctcgttgcc
      901 acgcgcgcgc ctgacgacac gcctcccgga gcacccactc gcccatcgga ataatcagat
      961 cagtctcctc tgcggcggca atgaactctc cgggcgggac gatcccgcgc tcaggatgct
     1021 cccacctgat gagcgcctcg aagcctgcgg cataaccagt ctcggttctc acgatgggct
     1081 ggtaatggag acggaactcg ccctcctcga ccgcccggcg taactccgac tccagccgca
     1141 gggtggtgac ggcgcgggcg tgcatcgtca ggtcgaagac ctcgtagcgt cccttgccct
     1201 tcgacttggc gcggtacatg gcggtgtccg cgtctcgaag catgtcatcc acgtttttgt
     1261 agccgagcga tgagagcgtg atgccgatgc tggccgaggt gacgatgccg tgatggccga
     1321 ggacgaaagg gagcgcgatc tcctcctgta tgcgctcggc ggcgcggacg gcgtccgtcg
     1381 aatgcctgat gccgttcagg aggatagcga actcgtcacc gccgaagcgc gccaccgtat
     1441 cccctgggcg gatgcaggtc ttaaggcgtt cggccaccgt cagcagtagt tgatcaccgt
     1501 gggcgtgtcc gagcgagtcg ttgacgttct tgaagcggtc gaggtccatg aagaggacgg
     1561 cgaagaggtg actgccgtgc cgctgggcgc gctccgcggc gagccgtaga tgatctgcga
     1621 agagggtgcg gttgacgagt ccggtcagcg agtcgtggtg agcggcgtgc tgcaaacgtt
     1681 cctcggcgtg cctgcgctcg gtcacgtcct gcacctgata gacgaggcgc aagggctgcc
     1741 ccttcgggtc gcgcacgagc gaggcgcata actgcgccca gagcgtacgc ccttctctgt
     1801 ggagcaggcg catctcaagg ttgtagctga tcgcgccccc ttgcgtcagc cggtagacct
     1861 gcgcgaggtg ctcgcctaac tcttccgggt gcaggacgga ttgtatgtcc gtcttgatta
     1921 attcttcctc ggcgtagccc agcatggcgc acagcgacgg gttgacctcg atccaagtgc
     1981 cgtctgtggc gacgagcgcc atcccgacgg cggcgttgtc gaaagagctg cggaactgct
     2041 cctcgctctg gcgcaattgc tggctgactg cccgctgctc ttcggcgtga cgctcggcct
     2101 gttcggcctg cgcgagcgta gcctgtaact gcttgcggta cgactggtag gtaaagtaaa
     2161 tgacggcgat gatgggtatc atcaccatca gggtgtagaa gccgtgcccg ttgacgagcc
     2221 ttgcggtaac tccagccgcc gaggcaccgg cgaagtaggt gatagaagtc cagaggtagt
     2281 gctctttcca gacctgtagg aacggcaggt tgagcttcat cgactggcgc aacgcaggga
     2341 tgccggagtt gactacgaac tgcacgcagc acatcaggca gacggctgtg atgaacctgc
     2401 cggaaagtgt ctcgcgcccc agttcgacga gaggaccgat ggcgtagccg acgactccgg
     2461 cggttacaaa ggtggagatc gcgagcagcc ccgcgttgaa gaacctcgtc agccacagct
     2521 tggcgaagcg aaaggacgag cagaaggctt cgacggctgc cagcagcacc gccgtctccg
     2581 tgccgtagag caatagcgtg aggaagataa agctgtcgga gacggtgatc tgtatcttgt
     2641 gtttggaaaa ctcgatgccg aggcgggagc cgaagcagag cgtgacggcg gtgaggatca
     2701 ggaagcgcag gtcgagccgg tcgaagggga gccggtagac gcagtacagg ctgacgaccg
     2761 cgccgagggc gggtatcagt cgcaggaaag gctggtggaa tcgttctctg ttcaagcggc
     2821 gtcttgcggt ttgtatggga tgttctattc gacctgcacg gctgcggggt tggcgaacac
     2881 gggtcgcgca tgtaggccag cccttcacta gcgagcggcg tgccagtaaa ccgtcgtgag
     2941 atataagagg atttggccgg aaatcgtccc acgcgtaggc atcgtgcaga cgcctggccg
     3001 tcagtctgac agggagccgg ttagcgaagt cagcttgcca gtcagactga tagcatgggg
     3061 agatgaacta atgatggtcg tgctaaagag gatgaccctt actgtctaag ggcgcggcct
     3121 atagatagac cttattgtac tctccgaggg agactgcgcc gaccctatcg tagaaactcc
     3181 gcgccgtaga gttctgggcg ttcacaaacc agcaaaaggc gggcgccttg tcgaggagcg
     3241 tgcggctcag agatgcccaa cagtttcgcc cgtacccttt gagccggtgt gccgggttaa
     3301 cccacacgcc ctcgatgtag gaggcttcgg gcgtctgcgt aaggacatcg gccttaaaga
     3361 tcaactcgcc cttatctatc agtgtccaga cctgaccctg ctcgacgcgc tgggcgcaac
     3421 gatggcgaaa tccctcagcg tcatcggcca gcgggttgac accactctcc tcgaagacca
     3481 tctgagcatg cgcctgaacg atttgatcca gatcgtcgag cgtagcctgc cggagtccgt
     3541 ccacacctgc ggcacggctg aactgtcggg aagactcata gaatatttca cggcagaata
     3601 gacgaggctc ctgtctacct gcgatatagc acctccagaa ggtttccagt tcgtcggcct
     3661 cgcccataat cattctgaca gccgggctgc gacgcgcccc tctggcgagg gcagagatag
     3721 ccgccccgtt ctgcgcctcg aacatggtgg cgcggcctac gagcgccaag ccttcgagtc
     3781 tgccccgcgc gtcgcggcag gtgtagaaca tgccctgtgc taacggcaca accggcccgt
     3841 aatggcgcag cagaccggcc atgatcaccg tatggcgggg gcgtcgggcg aggaaggcga
     3901 gcgcctcatc tctgtcctca ttaagcagtg gctccacatg tgtcgagtgg ctcttgttta
     3961 tagttacggg cacaggcatc gttgatctca gaccgtttct gcgtgtgagg ctggtgcggt
     4021 tgcgtatttg atggtcggtg ttgctcatgc ttaatcaggg tgaacgatca ggttcggaat
     4081 tgggtgccgg gaggcagcat ggtttatgtt tcgatgccgc ctcttcaatg ctttcaaagc
     4141 gcgcgagccg gggcggaaca agaacggacg cgcatcaggt tcacggttcg ctgccgtctt
     4201 cgtaaactac ggacatcccg ttcgtattgt cgccgttgat gagtacgtta agtgcccaaa
     4261 agctgtcgga gatcatgatg ccgtcggaga tcatgatgcc gtcggaaatc atgatgccgt
     4321 cggctaaggc gaagccgtcg gagatcataa ttccgtccga gatcatgatg ccgttgatga
     4381 agagtgagcc gccgcccatc ctgaggccat ccgagatcat aattcc
//
LOCUS       DASWNS010000339         6166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_39413, whole genome shotgun sequence.
ACCESSION   DASWNS010000339 DASWNS010000000
VERSION     DASWNS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39413"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VGB76_11860"
     CDS             complement(<1..966)
                     /locus_tag="VGB76_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099635.1"
                     /translation="MSKDRAFRQSLLFSLICCAAFVIMSASTTRAAVHDKPPASPTPM
                     PLPPITTQATPTPTPTPSPTPDPHQDQGLDDPDIGDPLLQDETPTEDASKPTTESDKA
                     FVSSDYKETHSDVEGGGTISPEPNSIPYDYSAPTNAPYPCERTIKASVVAFDQVFFWN
                     RLGAVQPQGMIYALRHNVVPINWRRGLSPGNVRLRRDKRPRPLVLRMNVGDCLQIDFQ
                     NLLNPTPVDEEQPVTRHASIHVVGLQAVNSIRDDGSNVGKNPSPPGGLVPPGGRITYT
                     LYADGRAKEGTHLMYSTAATTTGEGNGGQIPPGLFGAVNVEPRDAE"
     gene            1738..3414
                     /locus_tag="VGB76_11865"
     CDS             1738..3414
                     /locus_tag="VGB76_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099636.1"
                     /translation="MREEQQAQAKPGVSLRRRLWWLIAGRFILALALFGLSLLLGRNG
                     AASGAGLDSLDSTLVFGLLALLLSALYAAVLRFSKAGLQLQAGAQIFCDVMLVTWLTG
                     MRGDLHSPYVALYIVIISVAGIFLGVRGALVTSVACAVCYTFVVFAVAFGWLEVNAGD
                     VTSASVPEVIGNVGLHDIAFFVVGLLASRLAERQAHSDVQLIEAKHALADLRALHERI
                     VESIRSGLVTTDLNGLIYTFNAAAEEITGYTAEALRGQDVSILFGALKVKIEESLRAA
                     AESTASPRYEADCLTAEGLRLRLGYSIFPLFGETGARTGLVITFQDLTQVRALEETSR
                     RQDRLAAVGRVAAGIAHEIRNPLAAMRGSIQVLRSEMDGDAAQAELMEIVLRESDRLN
                     HIITDFLTYARPRPVSLADTDLREPLRETFTLLRHSPETRDGHIIEEDFPDTPVRAQA
                     DAAGLRQVFWNLSRNALSAMPEGGKLRVELHPTNYERVRITFTDTGAGMSPEQVERLF
                     EPFSSSTTGGTGLGLSIVYQIIRDHGGTINVRSREGHGTTIIIELPENRK"
     gene            3530..4942
                     /locus_tag="VGB76_11870"
     CDS             3530..4942
                     /locus_tag="VGB76_11870"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0099637.1"
                     /translation="MANILIVDDEQGMRQLLSLVFGRAGHAVHAAENGRRAVELLRES
                     PADLIVSDVRMPDMGGIDLLRAARELAPDVAVVMMTAFATVETAREAFKLGADDFIQK
                     PFDVDELKLIVEKALELRQLKQEIQGWRKTQSERGRLDQIIGRSPRMQAVYQMIETVA
                     AVQSTILITGESGTGKELVARAVHNLSPREDKPFVSINCGAFTETLLESELFGYVRGA
                     FTGATANRKGLFEAADRGTIFLDEIGEMSAAMQVKLLRVLQERKVRPVGGHEELSVDA
                     RVIAATNRDLPAMVKAGTFREDLFYRVSVIPIELPPLREKREDIRELVMHFAEKYSAQ
                     AGRNLTVSEGAMELLERYSWPGNVRELEHTIERAVALERTDQIQPERLPEQITNYNPA
                     RVADAFDLPEDGLNLTAHLDQLEKTYLLEALRRTGGNQTRAAELLQLSVRSLRHLLDK
                     HGIRNLTAQIRNDAARGAHE"
     gene            5151..5594
                     /locus_tag="VGB76_11875"
     CDS             5151..5594
                     /locus_tag="VGB76_11875"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY0099638.1"
                     /translation="MKAFSPEKSQRGFSLIELLIVVAVIGIISAIAVPHFVNSRQAAH
                     NASAFSSLRLVHMAQISYRMRHPQYGDLNALRNANMIDDPLLANGARSNYTFVINAAT
                     ITPSFYEATATPAVAPWQYYYLDVSGVIRREVGRAADITSSPINY"
BASE COUNT         1203 a         1864 c         1837 g         1262 t
ORIGIN      
        1 ttcggcgtcg cgcggttcga cgttgaccgc gccgaacagc cccggcggaa tctgcccgcc
       61 gttgccttcg cccgtcgtcg tcgccgccgt gctgtacatc aggtgcgtgc cttccttcgc
      121 gcgcccgtcg gcgtagagcg tgtaggtgat gcgcccgccg ggcgggacga ggccgccggg
      181 aggactcggg ttcttgccca cgttcgaacc gtcgtccctg atgctgttga cggcttgcag
      241 gccgacgacg tgaatcgaag catggcgcgt gacgggttgc tcctcatcaa ccggcgtggg
      301 gttgagcagg ttttgaaaat cgatctgcaa acaatcgccg acgttcatgc gcagcacgag
      361 cggtcgcggt cgcttgtcgc ggcgcaggcg gacgttgccg ggagagagtc cgcgccgcca
      421 gttaatcggg acgacgttgt gccggagcgc gtagatcatg ccctgcggtt gaaccgcgcc
      481 gagacgattc cagaagaaga cctgatcgaa ggccacgacg ctcgccttga tcgtgcgctc
      541 acaagggtaa ggcgcattcg tcggcgcgct gtaatcgtag gggatggaat tcggctccgg
      601 cgagatcgtg ccgccgcctt cgacatcaga atgagtctct ttgtaatcgg atgagacgaa
      661 ggctttatcg ctttccgtcg tgggcttcga agcgtcctcg gtgggcgtct catcttgcag
      721 cagaggatcg ccgatgtccg ggtcgtcgag tccctggtcc tgatgcgggt cgggcgtggg
      781 cgatggagtc ggcgttggcg tcggcgtcgc ctgcgttgtg atgggcggca gcggcatcgg
      841 cgtgggagag gccgggggct tgtcatgcac ggcggcgcgc gtcgttgagg ccgacatgat
      901 gacgaaggcc gcacagcaga tcaaagagaa caggagcgat tggcggaagg cacgatcttt
      961 agacataaac tcacctcttg cgctgactcg gtcgaacgtt cgaatcgcat aaaccgcaac
     1021 gatgcgaagg ctgttaaccg gagatgtgtt taaggagagt ttcgtctgaa gcgggtgtgg
     1081 ctcgggcgca tcaggcggaa ggctctcccc ggcgaagata agggttgagc ggttgccaca
     1141 agatgcgcta agatgcgggc tgtccggcgt tacatctttt gtgcgcagcg atgtggcgga
     1201 agttgttgac gcaaaactgt ccccgttgtc acctgcaagt gacggcgggg acaactcatt
     1261 taaccgaaag ttttgctgaa tgtttctttc aacatgacga ggtttagcat tcgataacgg
     1321 actatagcgg ctgcggcctg ctactcgatg aaggggcact gactaccaat ttaaacggta
     1381 cagcgggaca cgatttctca aagcacaggc ttagagttgc aagtggtcgg gaggttctcg
     1441 cttcaaaact tttcaagggg tgcacgcact ttaaacttca cctgcgttgc ctgtcaagag
     1501 ttatttttta tcaacattca agttacattt agctgctata ttttatgcaa accgcgctcc
     1561 tataaccgaa ccgcaacaat aatttcccac cctaactttc ccctgaacag gctgtgccga
     1621 cgcctcttcg cgtcgcttcg acaaggctgc gggtcgtcgc cgattgagcg tgatgttaac
     1681 tatcggcgga gatgtttttc cggtcgcccg ccgtttctgt gttaggcttt aaatgtgatg
     1741 cgagaggagc agcaggcaca ggcaaaaccg ggcgtttcgt tgcgacgcag gttgtggtgg
     1801 ctcatcgccg gtcgtttcat tttggctctg gcgcttttcg gtctgagcct gctgttggga
     1861 cgaaacggcg cggcgagcgg cgcggggctt gattcgctcg acagcacgct tgtcttcgga
     1921 ctgctcgcgc tcctgctctc cgcgctttac gcagcggtct tgcgcttctc gaaggcgggg
     1981 ttgcagttgc aggccggagc gcagatcttc tgcgacgtga tgctcgtcac gtggctcaca
     2041 gggatgcgcg gcgatctgca ttcgccctat gtcgcgctct acatcgtgat catttccgtg
     2101 gcgggcatct ttctcggcgt gcgcggcgcg ctcgtcactt ccgtcgcttg cgccgtctgc
     2161 tacacgttcg tcgtgtttgc ggtcgcgttc ggttggctgg aggtgaacgc gggcgatgtc
     2221 acctcggcct ccgtgccgga agtgatcggc aacgtcgggc tgcacgacat cgcgttcttc
     2281 gtcgtcgggc tgctcgcctc gcgtctggcc gagcgacagg cgcactccga tgtgcaactc
     2341 atcgaagcca agcacgcgct cgcagacctg cgcgcgctcc acgaacgcat cgtcgaatcg
     2401 atccgttccg gcctcgtcac gaccgacctc aacggactca tctacacctt caacgccgcc
     2461 gccgaagaga tcaccggcta cactgcggaa gcgttgcgcg ggcaggacgt ttcgatcctt
     2521 ttcggcgcgc tcaaggttaa gatcgaggag tctctgcgtg cggcggcgga gagcacggcc
     2581 agcccgcgct acgaagccga ttgtctcacc gcagaaggct tgcgcctgcg cctcggctac
     2641 agcatcttcc cgctcttcgg cgagacgggc gcacgcaccg gcctcgtcat cacgtttcag
     2701 gatttgacgc aggtgcgcgc gctcgaagag acttcgcggc ggcaagaccg gctcgccgct
     2761 gtggggcgcg tcgccgccgg gatcgcgcac gagatacgca acccgctcgc ggccatgcgc
     2821 ggctcgattc aagtcttgcg ctccgagatg gacggcgacg ccgcgcaagc cgaactcatg
     2881 gagatcgtcc tgcgcgaatc cgaccggctc aaccacatca tcacggactt tctcacctac
     2941 gcgcgcccac gccccgtctc gctcgccgac acggatttgc gcgaacccct gcgcgaaact
     3001 ttcacgctcc tgcgccacag cccggagacg cgcgacggcc acatcatcga agaggatttt
     3061 cccgacacgc ctgtgcgcgc gcaggccgac gcggcgggct tgcgtcaggt cttctggaat
     3121 ctctcgcgca acgcgctctc ggccatgccc gaaggcggca agttgcgcgt cgaactccat
     3181 ccgaccaatt atgagcgcgt ccgtatcacg ttcaccgaca cgggcgcagg catgtcgccc
     3241 gaacaggtcg aaagattatt cgaacccttc tcctcttcga ccaccggcgg cacgggactc
     3301 ggcctctcga ttgtttatca aatcatccgc gatcatggtg gtacaataaa cgtccgcagc
     3361 cgcgaagggc atggaacgac gatcatcatc gagttacctg agaatcggaa gtgatgagtg
     3421 ataagtctcg gaagtgatga gtgatgagta atgagtgatg agtcaaagac aaggttgttg
     3481 ttttgactca tcactcatca ctcatcactc atcactcatc atttgagtta tggctaacat
     3541 cttaatcgtt gacgatgaac agggcatgcg gcagttgttg tcgctcgtgt tcgggcgcgc
     3601 aggccatgcg gtgcacgcgg cggagaacgg gcggcgcgcc gtcgagttgt tgcgtgagag
     3661 tccggctgac ctgatcgtct cggacgtgag gatgccggac atgggcggca tcgatctatt
     3721 gcgcgcggcg cgcgagcttg cgcccgatgt cgcggtcgtg atgatgacgg cctttgccac
     3781 cgtcgagacg gcgcgcgagg cgttcaaact cggcgcggac gatttcatcc agaagccttt
     3841 cgacgttgac gagttgaagt tgatcgtcga gaaggctctg gagttgcgcc agctcaagca
     3901 ggagatacag ggctggcgca agacgcagag cgagcgcggg cggctcgatc agatcatcgg
     3961 tcgctcaccc cggatgcagg ccgtctatca aatgatcgag acggtggccg ccgtgcaatc
     4021 caccatcctg atcacgggcg aatcgggcac gggcaaggaa ctcgtcgcac gcgccgtcca
     4081 taacctctcg ccccgcgagg acaagccctt cgtgtcgatc aactgcggcg cgttcaccga
     4141 gacgctgctc gaatccgaac tcttcggcta cgtgcgcggc gcgttcacgg gcgcgaccgc
     4201 aaaccgcaag ggtctgtttg aagcggcaga tcgcggcacg atctttctcg acgagatcgg
     4261 cgagatgtcg gcggcgatgc aggtgaagtt gctgcgcgtg ctgcaagagc gcaaggtgcg
     4321 ccccgtgggc gggcatgaag agttgagcgt agacgcgcgc gtgatcgcgg cgacgaaccg
     4381 tgatcttccg gcgatggtca aggcgggaac ttttcgtgaa gacctcttct atcgcgtctc
     4441 ggtcatcccg atagagttgc cgccgctgcg cgagaagcgc gaggacatac gcgaactcgt
     4501 gatgcacttc gcggagaaat attccgcgca ggccggtcgg aatctaaccg tctcggaggg
     4561 agcgatggag ttgctcgaac gctattcatg gccgggcaac gtgcgcgaac tcgaacacac
     4621 catcgagcgc gccgtcgcac tcgaacgcac agaccagatt cagcccgaac gtctccccga
     4681 acagatcacc aactacaacc cggcacgcgt cgccgatgcg ttcgacctgc ccgaagacgg
     4741 cctcaacctc acggcgcatc tcgatcagtt agagaagact tacctgctcg aagccctgcg
     4801 ccgcaccggc ggcaaccaga cacgcgccgc cgaactgctc caactctccg tacgctcgct
     4861 gcgacacctt ctagacaagc acggcatccg caatctcacc gcccaaattc gcaacgacgc
     4921 cgcacgcggc gcgcacgaat aaggccagag gaaagaggaa gaggaaaggc tgaggggtat
     4981 aagggtaaag gtaaaagcag agcggcgatg cggcgcgtct gctctctcct ctgcccttta
     5041 ccccttagtc tttcccgttt ctcttcgctg gcatgctcat tgctcaagcg tcggtgagat
     5101 tgactgaaag gtcagtcaat ttgatgacgc gaggttgaaa cggcaaactg atgaaggctt
     5161 tctctccaga aaaaagtcaa agaggcttct cgctcataga gcttttgatc gtcgtcgcgg
     5221 tcatcggcat catctccgcc atcgccgtcc ctcatttcgt gaattcgcga caggccgccc
     5281 ataacgccag cgcgttcagt tcgctgcggc tcgttcacat ggctcaaatc tcctaccgga
     5341 tgcgccatcc tcagtatggc gacctcaacg cgctccgcaa cgcaaatatg attgacgatc
     5401 cgctgctcgc aaacggagcg cgcagcaatt acaccttcgt tatcaatgcc gcgaccatca
     5461 ctcccagctt ttacgaagcg acggcgaccc cggctgtcgc gccctggcag tactactatc
     5521 tcgatgtttc gggagtcatt cgcagggagg tcggaagagc ggcggacatc acgagttcgc
     5581 cgatcaacta ttgatggtgc gactgccgcg caggccgtcg cttatctccc gctctctcac
     5641 ctgctctcgc ccgcacgagt cctgatctta tctctccccg gacaaattga tcagccggac
     5701 gccatccgaa actgcacgga tgcgccggac gaaactgcgc ggatgtctcg cacatccgcc
     5761 gcaccttctt ttgacaaaat ccgtcacatt catctgacgc gttttgttaa tcgttccttg
     5821 acgcctcgct tcccggccag cccttcaacc gtctccacac gaaaaagccg cgattcttaa
     5881 gcctgtttgc gccgggcgcg cgggcgcggc acatgtggca tgactcttgt taactcagaa
     5941 gttcagcacg gcgagtagcc tacatagatg gcaaccgttg gcgcgtgaac ttaaaaacaa
     6001 ccgattccct ttgccataag gagcaaacta agaaatgcaa cagaacaaag gccaacgagg
     6061 cttctcactc atcgaactcc tcatcgtcgt cgcgatcatc ggcatcatcg ccgccatcgc
     6121 catccccaac ctgctcgcct ccaagcgttc cgctaatgaa ggctcc
//
LOCUS       DASWNS010000340        11389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_64558, whole genome shotgun sequence.
ACCESSION   DASWNS010000340 DASWNS010000000
VERSION     DASWNS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11389
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64558"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..801)
                     /locus_tag="VGB76_11880"
     CDS             complement(100..801)
                     /locus_tag="VGB76_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099639.1"
                     /translation="MMKRRDLMRRFGRSARVLLLSLALYPVPSTLLTPPASAQEVVDR
                     WVATINNRELITYTDLLWQLALQPDTPIDSPRSEDLQRVLNLLIDQRLISQEAGKLPT
                     ITPSDADVDKATNDLVKRFPSQAAFYERLARVGLTGEGLREIVRQRVEIENYLNFRFR
                     SFTIISTQEIADYYRDVFVPRHRRASPGRIVPTLEQATPEIERTLTENKIESDTDAFL
                     EDARTRADIVILNPV"
     gene            complement(834..4499)
                     /locus_tag="VGB76_11885"
     CDS             complement(834..4499)
                     /locus_tag="VGB76_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY0099640.1"
                     /translation="MLSRRFILVFLLLVACGAPVNGAPAATAVATAAAAVARMESVAD
                     LSRYEGRSIASVEVVFEGAQRNATAEAELRTLLTVAPNTPFSAVRVRESLQALFDSGR
                     VENVRVEASEVRSAAGLGGATVPVALRFFVRPQIRVTDVRLDLVGALPEGAPITADEL
                     RARLNLLDPGARVSDQSLRAGADAIQVYLRDRGFFRADVSFTRTLDAAGTGAIVSYTV
                     TPGEQARVEAFNIDVNGFNDSTVRPTLKLAPSAPFTRSALGEDIGRIRRAIMEMGYLA
                     PQLGDPQTVIDSARNTITVNLTGGIGPRVQVEVTGFEVSDKRRQELLPVVREGSIDQA
                     AITEGARRLENHLQEQGFFFADVIATCAVDRPAPPAPTTTTTTDPATATNTGANPNPT
                     PNPTAAAPPANANPNTVTPATTTGTTGVNPAPATTTAATVNGNIITSDPLAQANVDPL
                     VLTGACDNLNARELSGRTVNINYTVERGRRFRLSEIRIEGTEKLTYDDLAPELRSEPA
                     SGILGIIPFLGSRRGYTSVELLQRDQRTVEARLRDLGYRRANVEVRQGVAIDSEDLII
                     TFAVTEGPLTRVAGIEMRGNQLYTTERLRQEPCRAANFRSEPCTIVEAAFARTLARAD
                     GERIRNLYARNGYIDAEVRVDIVELPKRGDDEQVRLVYNITEGGKVFINRIIINGNEG
                     STPDARMRTKRDAILEAIPLREGEVLRSDRLAEAERVLYETNAFRQVIIRTEAAGETA
                     AGFRRRDVIIDLEENKPRRMDYGGGYSTDSGPLGLFELRNNNLFGGLRQGALRTRASR
                     RQQLFRLEYFDPRFRTYGRQRFSPLTVSAQYQRDINVTRFFRSTIDRGNFGIVQRLNE
                     EGNPIDEFGQQVGEPTINRFTFNIETQRVIQQRSRTILFLRYNYEDVRLLNIQSLLVA
                     PILRPDRAVRLSRFGATFTRDTRDRQFDPSRGEFLTVDYAVAIKQLGGNLSFTKLLAT
                     YRRYFRFGGGPDTSLVTDRPAGLLGGLTQSVRGTVFAVNVNLGIAQLFNPTDRNGDGR
                     IDDVDLTLPISERFFTGGSTTLRGFGFEEAGPRLSVCPTGTTLDTQTNSGNTCVGGVF
                     RNTSGDPVTLNPFTVPIGGNAMAVVNLEARVPLTKSFQIVPFYDGGNVFRRIGDIFGR
                     NDRRPDESLNMRNLRVEWTHTVGLGLRLRTPVGALGVDYGFLLNPPEFELPQANGIPA
                     IIRPRRAQLHFRFGQAF"
     gene            complement(4536..9515)
                     /locus_tag="VGB76_11890"
     CDS             complement(4536..9515)
                     /locus_tag="VGB76_11890"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY0099641.1"
                     /translation="MPVDDRDERSTGSSPDTHESETPQTEEPLARDDEPAPASASTPA
                     ERRRWRRYINRRNAMFAGIAVVAGIVLLVIIAFLLYRTGRVDDLIARQIVNTLKEYNI
                     RAEIREFHTKFGPRTVEMLGLDLYDGTTNEKLGHVDRIVATIRIEDMYALNLRRNINL
                     ESLQIEGLEAWVKFDEAGLSNFRNIRLPPPAENQRINFSYSTARIELRNGVVHYGDER
                     HELSGEARNLRATIEPDDPNAPAESRMNRVHLALADSTFTYDGKPIDKIDIEARARVN
                     QMRAEIQELTLKSPVAEARLEGTMDDWRNLRYQLKINSTVDLTQISNTLQTATPLRGA
                     GKFEGTVSGEGDKFQLDGQVQSDALAAGNIRLQGLNATARGTAQGVENYEAQGRAVAE
                     MLTAGNFTLNLIQIRGGVMGTGSDFRFLGELRAAAARSGATSIANLILSDAEAEYRDG
                     QINGNIPRLAAGNIRTSGAQVAGAQASGVRFNASEDGKTFNGSASSVNAGTITTGGAR
                     INGASVSGITAEGRNGVTNVLARSVVVRGIAAAGATLGQINIAGVRLAIQDSGRVEGS
                     SGDINVGTVAFRNGATPGRADNVRVARPTFLLEPSGRYRASADLSLGGGVLGDIALGA
                     ARSAVVATNSEIQLNNFTAEALNGRARGNAVISTAARSGTSRVNAQFEGLDVGGLLAV
                     LTGRAVPIAGAATGTIDLSMPGTNFEAASGTLNTQFTGETGNDANGRTPLTGELALVA
                     NRGQFQLERANLRTGASELTASGQFSFRGDSNLNVNLASNDAAELQRVLVASNLIPAL
                     EESLSSYGVGLAGRLDFNGTIRGALDAPLVNGRASLATLLVNGRDLGSLAASIESTPN
                     AVRVPDGRLTQRDGGNAQFALNYPLDGSNDGSLEATLERIDIGSLLAALPTSSAGAYS
                     SQLATVGPTSGRVSVRGIPNALSGSADVRSGPGSINGEPFDEIVARATFSGTTVTLEN
                     LDARFRAGRITAKGTVDTETQIFDLQAQGSGVNLAALAGLSGGAAPPVSGMADFTATA
                     RGNFADPRTFDVQINGEGRNVMINGQSAGTLAIVGRTENQRFNLQLTTGILGQPQVVN
                     AVVDLSNEDLNTTIETSLTNQDLTQLFATLMPNSNVRVTGRATGTLRAAGKLFGENEE
                     GERAFSLNGLRGTAQFSQLVFQIADVQLVAADPLIVQFSPNEIVFDRTQFTGPGTNVV
                     FGGSAALGAGGRQNFTINGELNLRVLNGISPDIFLAGTSRLEVRVGGTFEDPRLTGTA
                     SVAGASFSTLLTEQRLTVQNIQGSVRFTADRAQIDSLTGTLGGGRVNVSGGALLVGFT
                     PTDFRFNVRGENLTVPLPQDIRATADANLILQGRAQAQLLSLSGLVNLRRAEYTEDIE
                     LADFINGRREATISESGGEGGEGGFGANLRLDLRVEGRDALVVRNNLADLVGSVNLHV
                     RGSVEEPIIGGRITVARGTLNFRNDRYELTRAYIDLPARADADPILNIQAEAEIKGYR
                     VIVGLTGPLSSPTAVVRSDPALPQSDVVALITTGDLNRSEEGGATLAQTGFGTATSLL
                     TDTLINNPVQKATDRLFGLNRFEIDPLIAGRGGASPTARLTVGRQINRNLSLTYSTNV
                     TTDQNQVLALEYRVSDRLSFIAQYEQGATNTLRSARDNFSFEIRFRKRF"
     gene            complement(9669..10829)
                     /gene="ribB"
                     /locus_tag="VGB76_11895"
     CDS             complement(9669..10829)
                     /gene="ribB"
                     /locus_tag="VGB76_11895"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY0099642.1"
                     /translation="MPFSSIEEAAADIRDGRMVIIVDDEDRENEGDLVCAAEKITPEI
                     INFMATHARGLICMPLTEERCDELHLTMQVADNTSFLGTAFTVSIEARKGVTTGISAA
                     DRAKTILTAVDPKTKPSDLARPGHIFPLRARKGGVLVRPGQTEASVDLARIAGLYPAG
                     VICEIMNPDGTMSRLPELREFARTHDLKIISVADLVRYRMGKETLVRRAAEAEINTVF
                     GRFQAVAFENVINSEVHLAMVMGEVKTEEPVLVRVHTANITGDVFGSLMDDTNFQLHT
                     ALEKIAAEGRGVVLYLKQRQHGLDLLHQLHTYALMRERNLTFEEASIETGYGANRDYG
                     VGAQILHELGLRRIMLLSNHPPKVTALEGFELEVVGHVPLGSPAKETMNAER"
     gene            complement(11045..>11389)
                     /locus_tag="VGB76_11900"
     CDS             complement(11045..>11389)
                     /locus_tag="VGB76_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099643.1"
                     /translation="GRFLSAEEHGGSWTIRIAYPPELARYLVFKGSVSVEGISLTVAA
                     LADEYFEIAVIPKTWAMTNLSHLRSGDAVNIEADIIAKYVERILTVGARQPEAAQTSG
                     LTVEKLADLGYR"
BASE COUNT         1923 a         3410 c         3883 g         2173 t
ORIGIN      
        1 atttgaaaca gtgacaagtg acgagcgaca agtgacaagt tgaataccaa aaacttgttc
       61 taacctgtca ctggtcactg gtcactcgtg gctgctccat tataccggat tgagaatgac
      121 gatgtcggcg cgggtgcgtg cgtcttcgag gaaggcgtcg gtgtcggatt cgatcttgtt
      181 ctcggtgagc gtgcgttcga tctcgggcgt ggcctgttcg agcgtgggca cgatgcgtcc
      241 gggtgaggcg cggcggtggc gcgggacgaa cacgtcgcgg taatagtctg cgatctcctg
      301 cgtggagatg atggtgaagg aacggaagcg gaagttgaga tagttttcga tctcgacgcg
      361 ctggcggacg atctcgcgca agccttcgcc cgtgaggccg acgcgcgcga gccgttcgta
      421 aaaggctgcc tgcgaaggaa agcgtttgac gagatcgttg gtcgccttgt ccacgtcggc
      481 atcggagggg gtgatggtcg ggagttttcc ggcctcctgc gagatgaggc gttgatcgat
      541 gaggagattg aggacgcgct gcaaatcttc ggagcgcgga gagtcaatcg gcgtatcggg
      601 ttgcaaggcc aactgccaga gcaggtctgt gtaggtgatc aactcgcggt tattgatcgt
      661 cgccacccag cggtcaacca cctcctgcgc cgaagcgggc ggagttaaaa gggtagaagg
      721 tacgggatac agggcaaggg agagcagaag gacgcgcgcg ctcctgccga accttctcat
      781 caaatctcgt ctcttcatca tcgcctctcc ccttttcccc ttccccttat aacttaaaac
      841 gcctgcccga agcggaagtg tagttgcgcg cggcgcggac ggatgatggc ggggatgccg
      901 ttggcctgcg gcagttcgaa ctccggcggg ttgagcagga acccataatc gacaccgagc
      961 gcgccgacgg gcgtgcgcag gcgcaggccg aggccgaccg tgtgcgtcca ttcgacgcgc
     1021 aggttacgca tgttcaagct ctcgtcgggg cggcggtcgt tgcgcccgaa gatgtcgccg
     1081 atgcggcgga agacgttgcc gccatcgtag aagggcacga tctgaaaact cttcgtcaaa
     1141 ggcacgcgcg cttcgagatt gacaaccgcc atcgcgttgc cgccgatggg cacggtgaag
     1201 gggttgagcg tgacggggtc gccgctggtg ttgcggaaga cgccgcccac gcaggtgttg
     1261 ccgctgttgg tctgcgtgtc gagggtcgtc ccggtcgggc agacggagag gcgtggtccg
     1321 gcctcttcga agccgaagcc gcgcagggtg gtcgagccgc cggtgaagaa cctctcgctg
     1381 atggggagcg tgaggtcaac gtcgtcgata cgaccgtcgc cgttgcggtc ggtcgggttg
     1441 aagagttggg cgatgccgag attgacgttg acggcgaaga ccgtgccgcg cacgctctgc
     1501 gtcaagccgc cgagcaggcc agccggacga tccgtgacga gcgaggtgtc cggaccgccg
     1561 ccgaaacgga aatagcgtcg ataggtggcg agcagcttgg tgaaagagag attgccgccg
     1621 agttgcttga tcgccacggc gtagtcaacc gtcaggaact cgccgcgcga agggtcgaac
     1681 tggcggtcgc gcgtgtcgcg tgtgaaggtt gcgccgaagc gcgagaggcg cacggcgcgg
     1741 tcggggcgca ggatgggcgc gacgagcagg ctctgaatgt ttaagaggcg cacgtcctcg
     1801 tagttgtagc gcaggaaaag gatggtgcgc gaacgctgct ggatgacgcg ctgcgtctcg
     1861 atgttgaagg tgaaacgatt gatggtcggt tcgcccacct gctggccgaa ttcgtcgatg
     1921 ggattgccct cttcgttgag ccgctgcacg atgccgaagt tgcctcgatc aatcgtcgaa
     1981 cggaagaagc gcgtgacgtt gatgtcgcgc tggtattgcg cggagacggt gaggggcgag
     2041 aatctctgcc gcccgtaggt gcgaaaacgc ggatcgaaat attcgaggcg gaagagttgc
     2101 tggcgacggc tggcgcgcgt gcgcagcgcg ccctgacgca ggccgccgaa gaggttgttg
     2161 ttgcgcagtt cgaagagtcc gagaggtcca ctgtcggtcg agtagccgcc gccgtaatcc
     2221 atgcggcgcg gtttgttctc ttcgaggtcg atgatcacgt cgcggcggcg gaagcctgcc
     2281 gccgtctcgc ccgctgcttc ggtgcggatg atcacctgac gaaaagcgtt cgtctcgtag
     2341 agcacgcgct cggcttcggc gagacggtcg gagcgcaaga cctcgccttc cctgagcggg
     2401 atggcttcga ggatggcgtc gcgtttggtg cgcatgcgcg cgtcgggcgt ggagccttcg
     2461 ttgccgttga tgatgatgcg gttgatgaag accttgccgc cttcggtgat gttatagacg
     2521 agacgcacct gctcgtcgtc gccgcgcttc gggagttcga cgatgtccac gcgcacttcg
     2581 gcgtcgatgt agccgttgcg cgcgtagaga ttgcgtatgc gttcgccgtc ggcgcgggcg
     2641 agcgtgcgcg cgaacgcagc ttcgacgatg gtgcagggtt cgctgcggaa gttggcggcg
     2701 cgacacggct cctggcgcag acgctcggtg gtgtacaact ggttgccgcg catctcgatg
     2761 cccgccacgc gcgtcagcgg tccttcggtg acggcgaacg tgatgatcaa atcctcgcta
     2821 tcaatcgcca cgccctgccg gacttcgacg ttcgctctgc gataacccag atcgcgcagg
     2881 cgtgcctcta ccgttctttg atcgcgctgc aacaattcga cgctcgtata gccgcgccgc
     2941 gagccgagga aagggatgat gccgaggatg ccgctcgccg gttcggaacg aagttcgggg
     3001 gcgaggtcgt cgtaggtcaa cttctccgtg ccttcgatgc gaatctccga gagccggaag
     3061 cgacgcccgc gctcgaccgt gtaattgatg ttgacggtgc gaccgctcaa ctcgcgcgcg
     3121 ttcaaattgt cgcacgcgcc ggtcaagacg agcgggtcaa cgttggcctg cgcgagcggg
     3181 tcggacgtga tgatgttccc gttaaccgtc gccgccgtcg ttgtcgcagg ggcggggttc
     3241 acgcccgtcg tgccggtcgt cgtggcggga gttacggtgt tcgggttagc gttcgctggc
     3301 ggcgcggcgg cggtcgggtt tggcgtgggg ttcgggttcg cgcctgtgtt cgtcgcggtc
     3361 gcggggtcag ttgtcgtcgt tgtcgtcggg gcgggcggcg cgggacggtc tacggcgcag
     3421 gtggcgatca cgtcggcgaa gaagaagccc tgctcttgga ggtggttttc cagtcgccgc
     3481 gcgccctctg tgatggccgc ctgatcgatg ctgccctcgc gcacgacggg caacagctct
     3541 tgcctccgct tgtcgctcac ctcgaagccc gtcacctcca cctgcacgcg ggggccgatg
     3601 ccgcccgtca ggttcacggt gatggtgttg cgcgccgaat caatgaccgt ctgcgggtcg
     3661 ccgagttggg gcgcgagata gcccatctcc atgatcgcgc ggcggatgcg cccgatgtct
     3721 tcgccaagcg cgctgcgcgt gaagggggct gacggcgcga gtttgagcgt ggggcggacg
     3781 gtcgaatcgt tgaagccgtt cacgtcgatg ttgaaggctt cgacgcgcgc ctgctcgccg
     3841 ggagtgaccg tgtaggagac gattgcgccc gtgcctgccg cgtcgagcgt gcgcgtgaaa
     3901 ctcacgtcgg cgcggaagaa gccacggtcg cgcaggtaaa cctgtatggc gtcggctccg
     3961 gcacgaagcg attgatcgga gacgcgtgcg ccggggtcga gcaggttgag gcgtgcgcgc
     4021 aactcgtcgg cggtaatggg cgcaccctcc ggcaacgcgc ccacaaggtc gagccgcacg
     4081 tccgtcacgc gtatctgcgg gcgcacgaag aagcgcagcg cgacgggcac cgtcgcgccg
     4141 ccaagccccg ccgcgcttct cacctcggac gcttcgacgc gcacgttctc cacgcgcccc
     4201 gaatcgaaca acgcctgcaa agactcgcgc acgcgcacgg cggagaaagg cgtgttcggc
     4261 gcaaccgtga gcagggtgcg caattcggcc tcggctgtcg cgttgcgctg cgccccctcg
     4321 aaaaccactt ccacggaggc gatgctgcgc ccctcgtagc ggctcaagtc cgccacgctt
     4381 tccatgcggg cgacagcagc agcagcagta gcgacagcag tagcggcggg cgcaccgttc
     4441 acgggcgcgc cgcacgcgac gagcagaaga aagacgagga tgaaacgacg agaaagcacc
     4501 ttgtttgttc tttgtcagca gtcaacgatc agttttcaga accgctttct gaagcgaatc
     4561 tcaaaactga aattgtcgcg cgccgaacgc agcgtgttgg tcgcgccctg ttcgtactgc
     4621 gcgatgaacg agagccggtc ggagacgcgg tattcaagcg cgagcacttg attttgatcc
     4681 gtcgtcacgt tggtcgaata cgtgagactc aggttgcgat tgatctgtcg cccgaccgtg
     4741 agccgcgccg tcggactcgc gccgccgcga cccgcgatga gcggatcgat ctcgaagcgg
     4801 ttgaggccga acagccggtc ggtcgctttt tgcacggggt tgttgatgag cgtgtcggtc
     4861 aacaaactcg tggccgtgcc gaagcctgtc tgcgcgagcg tcgcaccgcc ctcttcgctg
     4921 cggttcaggt cgcccgtcgt gatcagcgcg acgacatccg actgcgggag cgcggggtcg
     4981 cttctcacca cggcggtcgg cgaagagaga ggccccgtta agccgacgat gacgcgatag
     5041 cctttgatct cggcctcggc ctgaatgttg agaatcgggt cggcatcggc gcgcgcgggc
     5101 agatcaatgt aggcgcgcgt caactcgtaa cggtcgttgc ggaaattgag cgtgccgcgc
     5161 gccaccgtga tacgcccgcc gatgatcggc tcttcgaccg agccgcgcac atgcaggttg
     5221 accgagccga cgaggtcggc cagattgttg cgcacgacga gcgcgtcgcg cccttcgacg
     5281 cgcaggtcca gccggaggtt cgccccgaag ccgccctcgc cgccctcgcc gccgctctcg
     5341 ctgatcgtgg cttcgcggcg gccattgatg aagtctgcga gttcgatgtc ttccgtgtac
     5401 tcggcgcgac gcagattgac gaggccggag agagagagca actgcgcctg cgcacggcct
     5461 tgcaggatga ggttggcgtc ggcggtggcg cgtatgtcct gcggcaacgg cacggtgaga
     5521 ttctcgccgc gcacgttgaa gcggaaatcg gtcggggtga acccgacgag gagcgcgccg
     5581 ccggagacgt tcacacgacc gccgccgagc gtgcccgtca gagaatctat ctgcgcgcgg
     5641 tcggctgtaa agcgcacgct gccctgtatg ttctgcacgg tcagtcgttg ttcggtgagc
     5701 agcgtcgaga aggacgcgcc cgccaccgag gccgtgcccg tcaggcgcgg gtcttcaaac
     5761 gttccgccga cgcgcacttc gaggcgcgac gtgcccgcga ggaagatgtc cggcgagatg
     5821 ccgttcagca cacgcaggtt gagttcgccg ttaatcgtga aattctgtcg cccgcccgcg
     5881 ccgagcgcag ccgagccgcc gaagacgacg tttgtgcccg gccccgtaaa ctgcgtccgg
     5941 tcgaagacga tctcgttcgg cgaaaactgc acgatgagcg gatcggcggc gacgagttgc
     6001 acgtctgcga tctggaagac gagttgggag aactgcgccg tgccgcgcaa gccgtttaag
     6061 gagaaggcgc gttcgccctc ctcgttttcg ccgaagagtt tgcctgccgc gcgcagcgtg
     6121 cccgtcgcgc gtcccgtcac gcgtacgttc gagttcggca tgagcgtggc gaagagttgg
     6181 gtcaaatcct gattcgtcaa actcgtctca atcgtcgtgt tcaaatcctc gttgctcaag
     6241 tcgacgaccg cgttgacaac ttgcggttga ccgaggatgc cggtcgtcaa ttgcagattg
     6301 aacctctgat tttcggtgcg cccgacgatg gcgagcgtgc ctgcgctctg cccgttgatc
     6361 atcacgttgc gcccttcgcc gttgatctgc acgtcgaagg tgcgcgggtc ggcgaagttg
     6421 cctctggccg tcgcggtgaa gtcggccatg ccggagacgg gcggagccgc gccgcccgaa
     6481 agccccgcga gcgcggcgag attcacgccc gaaccctgcg cttgcagatc gaagatttgc
     6541 gtctcggtgt caaccgtgcc tttggccgtg atgcgcccgg cgcggaaacg cgcgtcgaga
     6601 ttttcgagcg tgacggtcgt gccgctgaaa gtcgcacgcg ccacgatctc gtcgaagggt
     6661 tcgccgttga tgctgccggg accggaacgc acgtccgcgc tgccgctcaa ggcgttcggg
     6721 atgccgcgca cgctgacgcg ccccgaagtg ggtccaaccg tagcgagttg cgaactgtac
     6781 gcgcccgcgc tcgaagtcgg cagcgcggcg agcagacttc cgatgtcgat gcgttcgagc
     6841 gtcgcttcga gtgagccgtc gttcgagccg tcgagcgggt agttgagcgc gaactgcgcg
     6901 ttgccgccgt cacgctgcgt caggcgtccg tcgggcacgc gcacggcgtt cggagtggat
     6961 tcgatggagg ccgcgagcga gccgagatcg cgcccgttga cgagaagcgt ggcgagcgag
     7021 gcgcgaccgt tgacgagcgg cgcgtcgagc gcgccccgga tcgtgccgtt gaaatcgaga
     7081 cgaccggcga ggccgacgcc gtagctggag agactctctt cgagcgccgg gatcaagttc
     7141 gaggcgacga gcacgcgctg gagttccgct gcgtcgttcg aggcgagatt gacgttgaga
     7201 ttcgagtcgc cccggaagga gaactgcccc gaagccgtca actcgctcgc gcccgtgcgc
     7261 aggttggcgc gctccaactg gaactgcccg cgattggcga cgagcgcgag ttcgcccgtc
     7321 agcggcgtgc gcccgttcgc gtcgttgccc gtctcgcccg tgaactgcgt gttgagtgtg
     7381 ccgctcgcgg cctcgaagtt cgtccccggc atcgagagat cgattgtgcc cgtcgccgcg
     7441 cccgcaatcg gcacggcacg cccggtcaaa acggccaaca ggccgccgac atccagacct
     7501 tcaaactgcg cattgacgcg cgaggtgccg ctgcgcgccg ccgtgctgat gacggcgttg
     7561 ccgcgtgcgc gaccgttcag ggcttcggcg gtgaaattgt tgagttggat ttcgctgttc
     7621 gtcgctacga ccgcgctgcg cgccgcgccg agcgcgatgt cgccgagcac gccgccgccg
     7681 agactcaaat ccgcgctcgc gcgataacgt cccgaaggtt cgagcaggaa ggtggggcgc
     7741 gcgacgcgca cgttgtccgc gcgtcccggc gtcgcaccgt ttctgaatgc gaccgtgccg
     7801 acgttgatgt cgcccgaaga gccttcgacg cgcccgctgt cctgaatggc gagccgcacg
     7861 cccgcgatgt tgatctggcc gagcgttgcg cccgccgccg cgatgccgcg cacgacgacg
     7921 ctgcgcgcca gcacattcgt cacgccgtta cgcccctcgg cggtgatgcc gctcacgctc
     7981 gcgccgttga tgcgcgcgcc gcccgtcgtg atcgttccgg cgttgaccga cgaggccgag
     8041 ccgttaaaag tctttccgtc ctcggaggcg ttgaagcgca cgccggacgc ctgcgcgccc
     8101 gccacctgtg cgccggaggt tctgatattg cccgccgcca ggcgcgggat gttgccgtta
     8161 atctgaccgt cgcggtattc ggcttcggcg tcgctcagga tcaggttggc gatggaggtt
     8221 gcgccggaac gcgcggcggc ggcgcggagt tcgccgagga aacggaagtc gctgcccgtg
     8281 cccatcacgc cgccgcgaat ctgtatgagg ttgagcgtga agttgcccgc cgtgagcatc
     8341 tcggccacgg cgcgcccttg cgcttcgtag ttctcgacgc cctgcgccgt gccgcgcgcg
     8401 gtggcgttca aaccctgcaa gcgaatgttt ccggcggcga gcgcatccga ttgcacctgc
     8461 ccgtcaagtt gaaacttatc gccctcgccc gaaaccgtgc cttcaaactt gcccgctccg
     8521 cgcaggggcg tcgccgtctg caaggtgttc gagatttgcg tgagatcaac cgtcgaattg
     8581 atcttcaact ggtaacgcag attgcgccag tcgtccatcg tgccttcgag gcgggcttcc
     8641 gccacgggcg acttgagcgt caactcctgt atctcggcgc gcatctggtt gacgcgggcg
     8701 cgggcttcga tgtcgatctt atctatcggc ttgccgtcgt aagtgaaagt ggaatcggcg
     8761 agcgcgaggt ggacgcggtt catgcggctc tcggcgggcg cgttcggatc gtccggctcg
     8821 atggtggcgc gcaaatttcg cgcctcgccg gacaactcgt gccgctcgtc gccgtaatgc
     8881 acgacgccgt tgcggagttc gatccgcgcg gtcgaataag agaagttgat gcgctggttt
     8941 tcggcgggcg gggggaggcg tatgttgcga aagttcgaca gccccgcttc gtcgaacttg
     9001 acccacgctt cgagtccctc gatctgcaaa gattcgagat tgatgttgcg gcgcaggttg
     9061 agcgcgtaca tgtcttcgat gcggatggtg gcgacgatgc ggtctacgtg gccgagtttc
     9121 tcgttcgtcg ttccgtcgta gagatcaagc cccagcatct cgaccgtgcg cggcccgaac
     9181 ttcgtgtgaa actccctgat ctcggcgcgg atgttgtatt ccttgagcgt gttgacgatc
     9241 tgccgcgcga tcagatcatc cacccgcccc gtgcgataga gcagaaaggc gatgatgacc
     9301 agcagcacga tccctgcgac gacggcgatg cctgcgaaca tcgcgttgcg ccggttgatg
     9361 taacgacgcc agcggcgacg ctcggcgggc gtcgaggcgg aggcgggcgc aggttcgtcg
     9421 tcgcgcgcaa gcggttcttc cgtttgcggc gtctcagatt cgtgtgtgtc aggtgaagac
     9481 ccggtggaac gttcgtccct gtcgtcaaca ggcatagctt tagcgatgaa gtatgaacgc
     9541 ggaacgatga actaagaacc gatctttgtc agttgtccgt cggacattgt tcaacccctt
     9601 ccttgataac ttacgtatga atcacaaaac aacggactgg tggagacgga cgaagaacta
     9661 tctggcattc atcgttccgc gttcatagtc tcttttgccg gcgaaccgag cggcacgtgg
     9721 ccgacgactt cgagttcgaa gccttcgagc gcggtcactt tggggggatg gttcgatagg
     9781 agcatgatgc ggcgcagtcc caactcgtgc aggatttgcg cgccgacgcc gtaatcacgg
     9841 tttgcgccgt agcccgtctc gatactggcc tcctcgaaag tgaggttgcg ctcgcgcatc
     9901 agggcgtagg tgtgaagctg gtgcaggagg tcgagtccgt gctgtctttg tttgaggtag
     9961 agcacgacgc cgcgcccctc ggcagcgatc ttctcaagcg cggtgtgcaa ctggaaattc
    10021 gtgtcgtcca tgagcgagcc gaacacgtcg cccgtgatgt tcgccgtgtg gacgcgcacg
    10081 agcaccggct cctccgtctt gacttcgccc atcaccattg cgagatgcac ttccgagttg
    10141 atgacgttct cgaaagcgac ggcctgaaag cgtccgaaga ccgtgttgat ctcggcctct
    10201 gcggcgcgac gcacgagcgt ctctttgccc atgcgataac gcacgaggtc ggccacggag
    10261 atgatcttca gatcgtgcgt gcgggcaaac tcgcgcagtt cgggcaggcg cgacatcgtg
    10321 ccgtccgggt tcatgatctc gcagatgaca cccgccggat aaagccccgc gatgcgcgcc
    10381 agatcgacgc tcgcctccgt ctgaccgggg cgcacgagca cgccgccctt gcgtgcgcgc
    10441 agcggaaaga tgtggccggg gcgtgcgaga tcggagggct tcgttttggg atcgacggcg
    10501 gtgaggatcg ttttcgcacg gtcggcggcg gagatgccgg tggtgacgcc tttgcgcgct
    10561 tcgatggaga cggtaaaggc cgtgccgaga aacgaagtgt tgtcggcgac ctgcatcgtg
    10621 agatggagtt cgtcgcagcg ttcttcggtt aagggcatgc agatcaggcc gcgcgcgtgc
    10681 gtcgccatga agttgatgat ctcgggcgtg atcttttcgg cggcgcagac gaggtcgcct
    10741 tcgttctcgc ggtcttcgtc gtccacgatg atgaccatac ggccatcgcg aatgtctgcg
    10801 gcggcttctt caatggagga aaaaggcatt tcgatctcta ttcttcgact gcatggttcg
    10861 gggagtggcg tgtaccgacg gttatggtaa ccgaaagcca gcatttttgc gactcacaat
    10921 tctgttgcaa gcagaatgcc ccacgcgaat cttcgcgcaa ggctctcagg agagggttgg
    10981 gcggcggaga ggggatgccg caaggccgcg tcatttagaa gaaatttccg gcggcaggcg
    11041 ttgcctaccg gtaaccgaga tcggccagtt tctctacagt cagccccgaa gtttgcgccg
    11101 cctccggctg ccgcgcgccc accgtgagga tgcgctcgac gtacttggcg atgatgtcgg
    11161 cctcgatgtt gaccgcatcg ccggagcgca ggtgcgagag attcgtcatc gcccacgtct
    11221 tcgggatgac ggcgatctcg aaatactcat ccgccagcgc ggccaccgtc agactaattc
    11281 cttcgaccga aaccgacccc ttgaagacca gataacgcgc cagttccggc gggtaagcaa
    11341 tgcgaatcgt ccaggagccg ccgtgctcct ccgccgagag aaaacgacc
//
LOCUS       DASWNS010000341         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_91447, whole genome shotgun sequence.
ACCESSION   DASWNS010000341 DASWNS010000000
VERSION     DASWNS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91447"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VGB76_11905"
     CDS             <1..377
                     /locus_tag="VGB76_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099644.1"
                     /translation="DRINNEVKEFRQAADRFRSRVGDNGNDRNRGYQEAQYLLQAANN
                     TERMLSRLRVDSRTYSDWRQIKQDLRTIANAYGQRYDGYDDDYRNGGYDPRNNRNRGR
                     DRDRNGRDDNWWRQLPNIIGRP"
     gene            530..1459
                     /gene="rsmI"
                     /locus_tag="VGB76_11910"
     CDS             530..1459
                     /gene="rsmI"
                     /locus_tag="VGB76_11910"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY0099645.1"
                     /translation="MSERSDDAAAPAADITDAADVTDGAGTLYIVATPIGNLEDITRR
                     ALRVLSEVDTIACEDTRHTRTLLNHFGIKTKTVSYHEHNEHERTGELVRRLASGAHVA
                     LVSDAGTPAINDPGFRLVRAAIERGLNVVSVPGATAFVSALVASGLPTDEFYFAGFLP
                     PRGHARRLRLAALRAIPATLIFYEAPHRILDALTDAREILGEREAAVARELTKLHEEI
                     ARGRLTDLIARFSGEHAARGEMVLLIDRHVIAEAAGAGAPQTRNIAALVAAFEAEGLD
                     PRAALKRAARELGLNRDEAYRRLVSERAQAKQE"
     gene            1505..1675
                     /locus_tag="VGB76_11915"
     CDS             1505..1675
                     /locus_tag="VGB76_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099646.1"
                     /translation="MHEERDEPGETFNQDQQEMFQNPDTSPGETRPPTPGALNPLPLT
                     TDQEDDDSAKEP"
     gene            complement(1872..>3528)
                     /locus_tag="VGB76_11920"
     CDS             complement(1872..>3528)
                     /locus_tag="VGB76_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099647.1"
                     /translation="RKRLQDSPAYRLNHEEVVKSLEEGIDYIENMSPSEAVADEHGAV
                     AAIVFERMRQDAETRKWKASGERVRLPARTVCVAAGTSPNTIYEREQPGTFKLDEWGE
                     FFAPHKVERGGDAGSNGDGKFRLVPAAKGERGFFTSYEKAGRFVSYYGDNHPVYAGNV
                     VKAMASAKYGYEEVCALFADEIAAVEHESGAGVEGLERESKRVGGGGEGEPENGFEPE
                     RGAARFARLVAKLDDELIARVVRVERLTPTIVDVVVRAPMQARKFHPGQFYRLQNYET
                     DARVAGDTKLTMEGLALTGAWVDKSEGLLSLIVLEMGASSRQCALLAPGQQVVVMGPT
                     GTPTEIPADETVLLAGGGLGNAVLFSIARALRENNCRVVYFAGYKKGEDLFKREEIEA
                     ATDQIIWATDTGAQIEPRRPQDRHFRGNIVQAMKAYAVGELGAPMFELREVERIIAIG
                     SDRMMAAVKTARHGVLAEHFNPAHVGVGSINSPMQCMMKEVCAQCLQRHVDPQTGAES
                     FVFSSFNQDQLLDHVDFKNLNDRLRANTVQEKLANLWLDKLLK"
BASE COUNT          682 a         1259 c          953 g          634 t
ORIGIN      
        1 aagatcgtat caacaacgag gtgaaagagt ttcgtcaggc cgcagaccgt ttccgcagcc
       61 gcgtgggcga caacggcaac gaccgcaatc gtggctatca ggaagcgcag tatctcttgc
      121 aggccgccaa caacaccgag cgcatgctct cgcgcctgcg cgtcgattct cgcacttact
      181 ccgactggcg acagatcaaa caagacctcc gcaccatcgc caacgcctac ggccagcgtt
      241 acgacggcta cgacgacgac taccgcaacg gcggttacga cccgcgcaac aatcgcaacc
      301 gtggccgcga ccgcgaccgc aacgggcgcg acgataactg gtggcgtcag ttgcccaaca
      361 tcatcggtcg cccgtaagcg tcgaagtcag gttaaacgag cgaggggaga caagcgggaa
      421 gtttaagtac aagcgggaag tttaagtaaa agtgaaggcc ggtctggcat accagaccgg
      481 ccttcacttt tgcgtcttta cttttagctt ttgtcttaca atagcccgca tgtccgaacg
      541 ctccgacgat gccgctgccc ctgctgccga tattaccgac gctgccgatg ttaccgacgg
      601 cgcgggcacg ctctacatcg tcgccacgcc catcggcaat ctggaagaca tcacgcggcg
      661 cgcgctgcgc gtgctctccg aagttgacac catcgcctgc gaagacacgc gccacacgcg
      721 cacgctcctc aaccatttcg gtatcaagac caagaccgtc agctatcacg aacacaacga
      781 acacgaacgc acgggcgaac tcgtccggcg tctcgcgtcg ggcgcacacg tcgcgctcgt
      841 ctccgatgcg ggcacgcccg ccatcaacga ccccggcttc cggctcgtgc gcgccgccat
      901 cgagcgcggc ctcaacgtcg tctctgtgcc gggcgcgacg gccttcgtct ccgcgctcgt
      961 cgcttccggt ctgcccacgg atgaattcta cttcgcgggt tttcttcctc cgcgcggcca
     1021 cgcgcgccgt ctccggctcg ccgcattgcg cgccatcccg gccacgctca tcttctacga
     1081 agcgccgcac cgcatcctcg atgcgctcac ggacgcgcgc gaaatcctcg gcgaacgcga
     1141 agccgccgtc gcacgcgaac tcaccaagct ccacgaagag atcgcgcgcg ggcgtctcac
     1201 agatttaatc gcgcgcttct ccggcgaaca cgccgcgcgc ggcgagatgg tgcttctcat
     1261 agatcgccac gtgatcgcag aagccgctgg cgcaggcgcg ccgcagacgc gaaacatcgc
     1321 cgcgctcgtc gccgcgtttg aagccgaagg actcgacccg cgcgccgccc tcaagcgcgc
     1381 cgcacgcgaa ctcggcctca accgcgacga agcctatcgt cgcctcgtct ccgaacgcgc
     1441 gcaggcgaag caggaataag ttttttgcca cacgtcaaag cggatcgaac agaggagagc
     1501 gaagatgcac gaagagagag atgaaccggg cgaaactttc aaccaagacc agcaggagat
     1561 gttccagaac ccggacacct cgcccggcga gacgcgcccg cccacgcccg gcgcgctcaa
     1621 cccgctgccc ctcacgacgg atcaagaaga cgacgactcg gcgaaggaac catgatccgc
     1681 tgttaacaag cgcgtagctc gacgacttga gagttggctc ctctaaaagc gaaggccgcc
     1741 ccggcagaag aggcgacctt cgtgtgagtt tgaatgatga gacttgagcg tcggcctgcg
     1801 ttttgttact cgctctttcc gccaaagaat ttcttcacta aagcggcgcg ctgagagcgc
     1861 gccgccgccg cttacttgag caatttatcc agccagagat tcgccagttt ctcctgcacg
     1921 gtgttggcgc gcaagcggtc gtttaagttt ttgaagtcga cgtggtcgag tagctggtct
     1981 tgattgaagc tggagaagac gaacgactcc gcgcccgtct gcggatcgac gtgtcgctgc
     2041 aaacactgcg cgcagacctc tttcatcatg cactgcatcg ggctgttgat cgagccgacg
     2101 cccacgtgcg ccgggttgaa atgttccgcc agcacgccgt gtcgcgccgt cttcaccgcc
     2161 gccatcatcc tgtccgaccc gatggcgatg atgcgttcca cttcgcgcaa ctcgaacatg
     2221 ggcgctccca attctcctac ggcgtaagcc ttcatcgcct gcacgatgtt gccgcgaaag
     2281 tgcctgtcct gcgggcggcg cggctcgatc tgcgcgcccg tgtccgtcgc ccagataatc
     2341 tgatcggtgg ccgcttcgat ctcttcgcgc ttgaagaggt cttcgccttt tttgtagccc
     2401 gcgaaataga cgacgcgaca gttgttctcg cgcaaggcac gcgcaatcga gaagagcacc
     2461 gcgttgccga gtccgccgcc ggcgagcagt acggtttcgt cggcaggtat ctccgtcggc
     2521 gtccccgtcg gacccatcac gaccacctgc tgccccggcg cgagcagcgc gcactggcgc
     2581 gacgacgcgc ccatctccaa cacgatcagg ctcagcagcc cttccgactt gtccacccac
     2641 gcgcccgtca acgccaagcc ctccatcgtc agcttcgtgt ctccggcgac ccgcgcgtcc
     2701 gtctcataat tttgcagccg gtagaactgg ccgggatgaa acttcctcgc ctgcatcggc
     2761 gcacgcacca cgacatcaac aatcgtcggc gtcaaacgtt cgaccctcac cacgcgcgcg
     2821 atcaactcat catccaactt cgcaacgaga cgcgcgaacc gcgccgcccc gcgctccggc
     2881 tcaaacccgt tctccggctc gccttcccct cctccgccga cacgcttcga ctccctctca
     2941 agcccttcca cacccgcgcc gctctcatgc tcgacagcag cgatctcatc cgcaaacagc
     3001 gcgcacacct cctcgtaccc gtacttcgca gaagccatcg ccttgacgac gtttccggcg
     3061 tacaccggat gattgtcgcc gtaataggag acaaaccttc ccgccttttc atacgaagtg
     3121 aagaacccgc gctcgccctt tgccgccggg acgaggcgaa acttgccgtc gccgttgctg
     3181 cccgcgtctc ccccgcgctc aaccttgtgc ggcgcgaaga actcgcccca ctcgtcgagt
     3241 ttgaaagtgc ccggctgctc gcgttcgtag atcgtgttcg gactcgtccc cgccgccacg
     3301 cagaccgtcc gcgccggaag cctcacacgc tcgcccgaag ccttccactt tcgcgtctcc
     3361 gcatcctgcc gcatgcgctc gaacacgatg gccgccaccg cgccgtgctc atctgcgacg
     3421 gcctccgacg gactcatgtt ctctatgtaa tcaatgccct cttcgagtga cttcaccacc
     3481 tcctcgtgat tcagacgata cgcgggcgaa tcctgcaagc gtttgcga
//
LOCUS       DASWNS010000342         4934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_272815, whole genome shotgun sequence.
ACCESSION   DASWNS010000342 DASWNS010000000
VERSION     DASWNS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272815"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VGB76_11925"
     CDS             complement(<1..453)
                     /locus_tag="VGB76_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099648.1"
                     /translation="MTTAIEQTLETYGIENWGAGYFGINRKGNLVVHPSDTDTKSSAD
                     LKEIIDDLQRRGINTPVLLRFPQLVTAQVRKLQRAFRNAIREFDYQGTHMCVYPMKVN
                     QQRAVVEEYLREGARYDFGLEAGSKAELYAAIGLEQPSDSLLVLNGFKD"
     gene            883..2307
                     /gene="glnA"
                     /locus_tag="VGB76_11930"
     CDS             883..2307
                     /gene="glnA"
                     /locus_tag="VGB76_11930"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY0099649.1"
                     /translation="MDAKTALKYAEEQGAKFLSLRFTDLVGAWHHLTYPLHQLDEGSF
                     EDGFGFDASSLRGWASIHESDMLLVPDPVRMWMDPFAEEPTLCLIANTVDPITKEGYG
                     LDPRSVAQRAESYLRFTGLADTVYFGPEAEFFIFDSVRYNNDQFSAGYTIESDEGHWA
                     TGRANDETHGPNLGYRIRPKEGYVPVPPTDTLSDLRSEISLILERCGITVECHHHEVA
                     TAGQCEIDFRYATMLGTADNLQIFKYVVRNTAHQYGKTATFMPKPLYGDNGSGMHCHQ
                     SLWKGEKPLFAGDGYAGLSEMARFYIGGLLKHAAAIVAFAAPTTNSYKRLVPGFEAPV
                     NLAYSARNRSAAIRIPMFSTNPKLKRLEFRPPDPSCNPYLAFSAMLLAGLDGIQNRID
                     PGEPLDKDIYDLSPEQLKDVPSLPGSLDESLNALEADHDFLLKGDVFTPQLIDRWITY
                     KRDNEITRLRMRPHPLEFSMYYDI"
     gene            2463..2936
                     /locus_tag="VGB76_11935"
     CDS             2463..2936
                     /locus_tag="VGB76_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099650.1"
                     /translation="MSEEMREERREEVVQPAPAKSSNLLQRIGLYAGLMLVAFLLGFV
                     PMWLKARESGNNLAAAESKLGLAQMQNNLASAVIDARRGDYEPARQAASQFYTSLRAD
                     LDKVDGSGFNEAQRNAAQALFAGRDEIITLLARSDPASADRLSDVYAAYRKLVSG"
     gene            complement(2944..3504)
                     /locus_tag="VGB76_11940"
     CDS             complement(2944..3504)
                     /locus_tag="VGB76_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099651.1"
                     /translation="MARVLAEFGTPPEWFRAQGFPTLVHIILEQQVSLASAEAAFKRL
                     CALASPLTPARFLKLDDAELKRAGFSRQKTAYGRHLARSIMEGELDLEAFGAMDDAAV
                     KAELLRVKGIGAWTADIYLLMALRRPDAWPAGDLALMIALQEVKRLPARPGAFELEVI
                     AERWRPYRAVAARLLWHYYLNRRAKR"
     gene            complement(3629..4105)
                     /locus_tag="VGB76_11945"
     CDS             complement(3629..4105)
                     /locus_tag="VGB76_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0099652.1"
                     /translation="MTEIERLADQLQRAFYADAWCGPSLLETLEGVNAVQAAARPLAR
                     AHSIWEIVSHVSGWKRTVHQRLEGQAVRLPEEGDWPPVADTSEESWQATLARLKSRHD
                     ALLAAVRGLDEARLEDVLITESSRETGGGVSCYVTLHGTAQHDLYHGGQISLLKKG"
     gene            complement(4102..4653)
                     /locus_tag="VGB76_11950"
     CDS             complement(4102..4653)
                     /locus_tag="VGB76_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099653.1"
                     /translation="MLNLIRKIFAAGYSLIILLFLCCAVALIVFALLELWEGINPNSG
                     LPLRERFNAVLECVGLLTIAVASLELGQTILEEEVIRASQMSAPTRVRRFLSRFLVVV
                     IVSLAIESLVAVFQFLHDKPEQLPQASMIAVAAAALLAAWGVFIKLNKSAEELEPEAM
                     EDAKREDRVVEEDEGARDAGRGA"
BASE COUNT         1005 a         1556 c         1363 g         1010 t
ORIGIN      
        1 gtccttgaaa ccgttcagga cgagcaggct gtccgaaggt tgttcgaggc cgatggcggc
       61 gtagagttcg gctttcgacc cggcttccag tccgaagtcg tagcgcgcgc cctcgcgcag
      121 gtactcttcg acgacggcgc gctgctggtt gaccttcatc ggatagacgc acatgtgcgt
      181 cccctgatag tcgaactcgc ggatggcgtt gcgaaaggcg cgctggagtt tgcgcacctg
      241 tgcggtaacg agttggggga agcgcaagag gacgggcgtg ttgatgccgc gacgctggag
      301 gtcatcgatg atctctttca ggtcggcgga agatttggtg tcggtgtccg aaggatggac
      361 gacgagattg cctttgcggt tgatgccgaa gtaaccggct ccccagtttt cgatgccgta
      421 ggtttcgagc gtctgttcaa tagccgtggt caacttccgg tctcctgcct ccctgtgtgt
      481 tcgtgtgtgg gcatcaagtg cgcgcccgct cagtctcacg cgccgtctgc gtaggaactt
      541 tcatggttgg aaaacttaag gtcgcagagt aacagaaaaa aggaaggcgg acaataacta
      601 tttttggcgc aaaaacgccc gcccgcgcgc ttaacccgtc gtctcagaga ggtttaggag
      661 actcgccgcc gctctgttga aaaccgtgtc acaaactttc tcgcgtgaaa ccttgacagg
      721 cgtgcgtgcg tctctataat ccgaactttt ttaagcgtcg ctctcaagta gtttcatgac
      781 gcccgctttg aagcatttgc cgaactctac aacagccgcc cgcacacacc gcaacccggc
      841 ggcctgtcag ccccacactc cctacaacat tcaggagatt acatggacgc aaaaacggcc
      901 ttgaagtatg ccgaagagca aggagcgaaa tttctctcgc tcagattcac agacctcgtc
      961 ggtgcgtggc accatttaac gtatcccctt caccaactcg acgaaggctc tttcgaggac
     1021 ggcttcggct tcgacgcttc gagtttgcgc ggctgggctt cgattcacga atccgacatg
     1081 ctgctcgtgc ccgaccccgt gcgcatgtgg atggacccgt tcgcggaaga acccacgctc
     1141 tgtctcatcg ccaacacggt cgatccgatc acgaaagagg gttacgggtt agacccgcgc
     1201 tccgtcgcac agcgcgccga gagctacctg cgttttacag ggctggccga tacggtttac
     1261 tttggtcccg aagcggagtt cttcatcttc gactcggtgc gctacaacaa cgaccagttc
     1321 tccgccggtt acaccatcga atccgacgaa ggccactggg cgacggggcg ggctaatgac
     1381 gagacacacg gacccaacct cggctatcgc atccgtccca aagagggcta cgtccccgtc
     1441 ccgccgacgg acactctgag cgatctgcgc tcggagatca gtctgattct ggagcgttgc
     1501 ggcatcacgg tcgagtgtca tcaccatgaa gtcgccacgg cggggcaatg cgaaattgat
     1561 tttcgctacg cgacgatgtt ggggacggct gacaatttgc aaatcttcaa gtacgtcgtg
     1621 cgcaacacgg cgcaccagta cggcaagacc gcgaccttca tgccgaagcc gctctacggc
     1681 gacaacggtt cgggcatgca ctgtcatcaa tcgctctgga aaggcgagaa gccgctcttt
     1741 gcgggcgacg gctacgcggg actctccgag atggcgcgct tctacatcgg cggcctcttg
     1801 aaacacgcgg cggccatcgt cgccttcgcc gcgccgacca cgaacagcta caagcggctc
     1861 gtccccggct tcgaagctcc ggttaatctc gcttactcgg cgcgcaatcg ttcggcggca
     1921 attcgcatcc cgatgttctc tacgaacccg aagttgaagc gtctggagtt tcgtccgccc
     1981 gacccgtcct gcaatcctta tctcgctttc tcggccatgc tcctcgccgg actcgacggc
     2041 atccagaacc gcatcgatcc cggtgagccg ctcgacaagg acatctacga tctcagtccg
     2101 gagcaactca aagacgtgcc gagcttgccg ggttcgctcg acgaatcgct gaacgcgctc
     2161 gaagccgacc acgacttcct gctcaaaggc gacgtgttca cgccgcaact catcgaccgc
     2221 tggatcactt acaaacgcga caacgagatc acgcgcctgc gcatgcgccc gcacccgctc
     2281 gaattctcga tgtactacga catctaaaca gtgagcagaa cgcaggatgc ggaacgatga
     2341 acgaaagcga gaatctttcg ttcatcgttc cgcattctcg tctttgcgtg cgacatctga
     2401 tactgtgtcg gttaaaggtt ccgcgaaggc aacacccgac ggtctacaag gagtcaagat
     2461 ttatgagtga agagatgcga gaagagaggc gcgaagaagt cgtccaacct gcgcccgcca
     2521 aatcgagtaa cctgcttcag cgcatcggcc tctatgccgg gctgatgctc gtcgccttcc
     2581 tcctcggctt cgtcccgatg tggctcaagg cgcgcgagag cggcaacaat ctcgcggcgg
     2641 cggagagcaa actcggcctt gcgcagatgc agaacaacct tgcctcggca gtcatcgacg
     2701 cgcggcgggg ggattacgag cctgcgcgcc aagcggccag ccagttctat acttccctac
     2761 gggctgatct cgacaaggtc gatggctccg gtttcaacga ggcgcagcga aatgccgcgc
     2821 aggcgttgtt tgccggacgc gacgagatca tcaccctgct cgcccgcagc gacccggctt
     2881 ctgccgaccg gctctccgac gtgtatgcag cttatcgtaa gctcgtgagc ggctgatccg
     2941 gcgtcacctc ttcgcccgcc ggttcaaata gtaatgccat aacaggcgcg cggcgacggc
     3001 gcgatacggt cgccaacgct cggcgataac ttcaagttca aaagctccgg ggcgcgccgg
     3061 gagacgtttc acctcctgca acgcgatcat caaggccaag tctccggcag gccacgcgtc
     3121 cggccttctc aaggccatca ggagatagat gtccgccgtc cacgcgccga ttcctttcac
     3181 acgcaacaac tccgctttca ccgccgcgtc atccatcgcg ccgaacgctt cgaggtcgag
     3241 ttcgccctcc ataattgagc gtgcgaggtg tctgccatag gcggtcttct ggcgactaaa
     3301 gcccgcgcgt ttgagttcgg cgtcatcgag tttgaggaag cgtgcgggcg tgagcggcga
     3361 ggcgagcgcg cagagacgct tgaaggcggc ctcggcggaa gcgagcgaaa cttgttgctc
     3421 taagatgatg tggacgaggg tggggaaacc ctgtgcgcga aaccactcgg gcggcgtgcc
     3481 gaactctgcg agcacgcgcg ccatgtcttc gtcgctcgcg gctaaagttc gcagcccttc
     3541 ggcgtaagac ttccggttta aaatcctctg cttgatttta cctcgcagcg cgccagcctt
     3601 tctttacccg tcagcctttc tttacgcgtc agcctttctt taacagtgaa atctggccgc
     3661 cgtgatagag gtcgtgctgc gccgtgccgt gcagcgtcac gtagcaagac acgccgccgc
     3721 ccgtctcgcg gctgctctcc gtgatgagca catcttccag acgcgcttca tcaagcccgc
     3781 gcaccgctgc cagcaaagca tcgtgccgcg acttcaaacg cgccagcgtc gcctgccaac
     3841 tctcttcgct cgtgtccgcc accggcggcc aatcgccctc ttccggcaac ctcacggcct
     3901 gaccttcgag ccgctgatgc acagtccttt tccaaccgct cacatgcgag acgatctccc
     3961 agatgctatg cgcccgcgcg agcggacgcg ccgccgcctg cacggcgttg acgccttcca
     4021 gcgtttcgag cagcgaagga ccacaccacg cgtcggcgta gaaagcgcgt tgcagttgat
     4081 ccgcgagccg ttcgatctca gtcatgctcc cctcccggcg tcgcgcgcac cctcatcctc
     4141 ttctacgaca cgatcctcac gcttggcatc ttccatcgct tccggttcca gttcctccgc
     4201 gctcttgttc aatttaatga agacgcccca cgcggccaac aacgccgctg ccgcgacggc
     4261 gatcatcgag gcttgcggca actgctccgg cttgtcgtgc agaaattgaa agaccgccac
     4321 cagcgattcg atagcgagcg acacgatgac gaccaccaga aagcgcgaca gaaaacggcg
     4381 cacacgcgtc ggcgcactca tctgcgatgc cctgatcacc tcttcttcga ggatggtctg
     4441 ccccagttcg agcgaggcca ctgcgatggt caacagcccg acgcactcca gcaccgcatt
     4501 aaaccgctcc ctcagcggca aacccgagtt gggattgatc ccctcccaca attcgagcag
     4561 ggcgaagacg attaaagcga cggcgcagca gagaaacaac aggatgatca acgaatatcc
     4621 cgccgcgaat attttccgaa tgaggttcaa catctctcct taaagaatac gatcgctctt
     4681 ttctaaattg tgccgggcac atagaagctg cacgttttcc ggcttcaagg atgtgccgcc
     4741 tttcgaatac ggtagtatgt ggtcaaaatg gagttcatcg gttgcactac acacaacgca
     4801 cctcccgtta tctcgcttcc atactttgag ctttacgttg gtcggtatca ttcgccgtct
     4861 gatgggattc attggggtgg gaaccgtaaa atcttcctca ccttcgacgg cgatcaactt
     4921 gaatttgaaa actg
//
LOCUS       DASWNS010000343         6850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_481716, whole genome shotgun sequence.
ACCESSION   DASWNS010000343 DASWNS010000000
VERSION     DASWNS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481716"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGB76_11955"
     CDS             <1..366
                     /locus_tag="VGB76_11955"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0099654.1"
                     /translation="WATWCPPCRAEMPDLVKLQREYGKDGLQIIGVTYQPEQRAHVRK
                     FTRGLKVNYPIVLGTRETKASFSRDESLPLTVIIDREGTIRGAIAGILLPEEFNLQIK
                     PLLQRRASERETNHVQKNK"
     gene            347..634
                     /locus_tag="VGB76_11960"
     CDS             347..634
                     /locus_tag="VGB76_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEY0099655.1"
                     /translation="MFRRISSFAVACAIVLAFSLTTAFADTKTTTIRVTGMKCNQCSS
                     SVAKALKATAGVQEAEVSFEKGQVVIIYDDEKVSEAKLREVINSTGFKVAE"
     gene            complement(1541..2059)
                     /locus_tag="VGB76_11965"
     CDS             complement(1541..2059)
                     /locus_tag="VGB76_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEY0099656.1"
                     /translation="MSLSTYVPGAPKRAVVERHERPEHTLQPTALVNEVYLRLIDQQR
                     VSWHNRAQFFGLAAQLMRHVLVDHARTRASVKRGGLVHKLSLDEAWMAPGEVAAELIA
                     LDEALVRLAAFDVRKSRVVEMRFFGGLSVEETAEALGVSDKTVMRDWRIARMWLYREL
                     SGSVEGDVELES"
     gene            2046..2543
                     /locus_tag="VGB76_11970"
     CDS             2046..2543
                     /locus_tag="VGB76_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099657.1"
                     /translation="MLRLTADDTEFARHDELTITVSGENQSPTVNADADQTITLPNTA
                     ALSGTTTDDGWPEGSPLTATWSVVSSPGTVAFNNPHQAATTASFGAAGNRQRRSNLDG
                     DRHGQHACQRWPAGSLRPDPYAQRHLYAAACRHRRSQPDGDLVNRRGGGRPAAGSFQL
                     TASNP"
     gene            complement(2953..4134)
                     /locus_tag="VGB76_11975"
     CDS             complement(2953..4134)
                     /locus_tag="VGB76_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0099658.1"
                     /translation="MKNEPVDTGESGERAELPAALSSEKRNVRLDPLVELTRARLREF
                     VREPEAVFWVFIFPVLLAFALGIAFRNTAPEKIRVAIESDRQKAGEPVSQLATALARS
                     GEIEVVSLSKDDAARALRSGKVTLVVRPVDAGAANEKVPGLTEQAAQAPFNYRYDPAR
                     PESRTARLLVDDALQRALGRRDVTSAGEERVSETGARYIDFLIPGLLGLNIMGSGLMG
                     VGFAIVTARMRKLLKRLAATPMSRSHYLLSFVFSRLILLVLEVGAVIIFARLVFSYRV
                     QGSFPSIVLLLLTGAFAFSGIGLLLASRATTLEGVSGLMNMVMMPMWLLSGTFFSSER
                     FPAFLQPFIKILPLTALNDALRAMMNEGTTLADNVTPLCVLLAWAVLSFIVALKVFRW
                     Q"
     gene            complement(4173..5108)
                     /locus_tag="VGB76_11980"
     CDS             complement(4173..5108)
                     /locus_tag="VGB76_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0099659.1"
                     /translation="MFKQVPLALHTVGLRKRYGEVTAVNGLDLEVRVGECFGLLGPNG
                     AGKTTTVEILEGLIKPDGGTVEVLGYRWGDRHAGDLRQRLGVQLQETKLAEKLTVEET
                     IKLFRSFYHDRRGVEELIRMVELEKKRDARVGKLSGGQKQRLALACALAGNPDILFLD
                     EPTTGLDPQARLKLWDIVEQFRAGGGTIILTTHYMEEAARLCDRVAIMDQGKIIALDT
                     PAALVASLGAEQIVEFSASREIREETLSALAGVRAVRRMSGVCSLVVSDISAALPALL
                     AAIERESAELLTLTTHQATLEDVFVSMTGRMLREK"
     gene            5321..5512
                     /locus_tag="VGB76_11985"
     CDS             5321..5512
                     /locus_tag="VGB76_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099660.1"
                     /translation="MNTPNDLIPLREARHLLGVSDNKVTSLVKAGLLKTYTDLLDRRK
                     KLVSRAEVEGLKVPRAEAA"
     gene            5509..6306
                     /locus_tag="VGB76_11990"
     CDS             5509..6306
                     /locus_tag="VGB76_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY0099661.1"
                     /translation="MKTIVLANAKGGTGKTTCAVNLSACLALHHGKRVLGIDLDPQGN
                     FGISYGVDPRQLTHTAYHMLTAESPDVREYVREIKPNLHLIPNAIAPDLENRLEASHN
                     RDSLLRLRLRQIKNQYDYVIIDTPPAMRTATMNAIVAADEVIVVVDCGFYALYGLTDL
                     MRQIARVSDAYEKDQIVIRGLLNMFNRGQNLDRDVKKEVSDFFGGLMIQTIIHKNVKL
                     AEAASASQSIIEFDSSAPGAFDFIKLSKELLAEYEPEEEKRSATRSK"
     gene            6263..>6850
                     /locus_tag="VGB76_11995"
     CDS             6263..>6850
                     /locus_tag="VGB76_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099662.1"
                     /translation="MSQKKKSGLQRGLSDIVEKQTAPTPDKSQLSRGLIGKFTEPKPT
                     LSTQGTNSTPRTLSTHPAPMRDFQKVANSIMREAVPAGVFKGKCKQIYDFLYSKTRGA
                     IVPARSAKLTRQAIMEGSHVGSTKTLFLNLRHLRTAGLVSWTECEGTHGGNEYTVYLP
                     EEALSTLSTQGTLSAPGYNLPMVPSVESTMGTQGLS"
BASE COUNT         1534 a         2098 c         1841 g         1377 t
ORIGIN      
        1 tgggcgactt ggtgcccgcc gtgtcgcgcg gaaatgcctg acctcgtcaa gctccagagg
       61 gagtacggaa aagacggcct gcaaatcatc ggcgtcacct accagcccga gcaacgggcg
      121 cacgtgcgga aatttacgcg agggctgaaa gttaactatc ctatcgtgct cggaacacgc
      181 gagacgaagg ccagcttcag ccgcgacgag tcgctgccgc tgacagttat catcgaccgt
      241 gaaggaacga ttcgcggcgc catcgcgggg attcttctcc ccgaagagtt taacctacaa
      301 atcaagccgc tgttacaacg gcgtgcatca gaaagagaga cgaaccatgt tcagaagaat
      361 aagtagcttt gcggtcgctt gcgcgatagt actggccttc agcctgacga cggcatttgc
      421 cgacaccaag acaacgacga tccgcgtcac gggcatgaag tgcaaccagt gctcctcctc
      481 cgtcgccaag gctctcaagg ccactgcggg cgtgcaggaa gccgaggtca gttttgagaa
      541 ggggcaagtc gtgatcatct atgacgacga gaaagtgtct gaggcgaaac tgcgcgaggt
      601 tattaacagc accggcttca aagtcgccga gtaacggaag cgaagtaaca aacgctgtac
      661 ttataagcct gataactcag cgtcgctgac gccgggcttg tggccgagta gacgttcgac
      721 gggacatctg acttctacct acagtgctgc ccggagccct gaacaatcac tccgcaccgg
      781 aatatcttgc acgagtcaac ttaaacgcat tcttccccgg cgacgcttcg ttagcctcac
      841 ctcactgtgt ctcaactcaa ggtaacttgt gcgaaggcca gcagagtccg ataagtatta
      901 cacaaatcat gtgggctttg cctaaaactc cctaaatctt aggcacttac cgggcaggcg
      961 agctagtctt cgagcaggta ttttaaggaa gggtaggcgc gggatgttag ccatcttaca
     1021 ccccgcgcct ttctactatt caagcgggca tgagtccacg caggtggtgt atgtctcgaa
     1081 gcaccgctgc ctttggcctg gcgaacaacc gctggtgcag tagtaggcgc agtattcgta
     1141 ttgtgcctga cactgttctt cgcaggtata ggaagccctg gccttttcca acggagaggt
     1201 agatgagagg gcggcgatta gtactgacag gattaagagt cggcgtgcga gtgggcgttt
     1261 ctgcataatg ttctccttcg ttgtgagtag cgtaagccgt tgaggttgag gggtcgtaac
     1321 gcaataggga acgcgagaac gatacgtctg acagagtggg gtgtcaacga ttaaaagaga
     1381 gaagagccgc gcagcgagag ccgcgcagcg atggttagag ctatcgtggc ttttatcggc
     1441 tgagacttcc cgcgctcggc ttgatagaac tgcctgaggt tatcggacgc tcgcttgatg
     1501 ctttacctat ctgccgctat tgttccgcct tcatcgctct ctaagactca agttcaacgt
     1561 cgccctcgac gctcccgctc aactcacgat acagccacat ccgggcgata cgccaatcac
     1621 gcatcaccgt cttgtccgag acgccgagag cttcagccgt ctcctccacg ctgaggccgc
     1681 cgaagaaacg catctccacc acgcgactct tccgcacgtc aaacgcggcg agcctcacga
     1741 gcgcctcgtc gagcgcgatc aactccgccg ccacttctcc gggagccatc cacgcttcat
     1801 caagcgacag tttgtgtacc agacctccgc gcttgactga cgcgcgcgtg cgggcgtggt
     1861 ctacgaggac gtgtctcatc aactgcgccg ccagcccgaa gaactgcgcg cggttgtgcc
     1921 aactgacccg ctgctggtcg atgaggcgca gataaacttc gttgacgagc gccgtcggtt
     1981 gcagagtgtg ttcgggacgc tcatgtcgct cgaccaccgc acgcttcggc gcgcccggca
     2041 catacgtgct caggctcact gccgacgaca cggagttcgc acgccacgac gagttgacga
     2101 tcacggtcag cggcgagaac caatcgccga ccgtcaatgc cgatgccgac cagacgatta
     2161 ccttgccaaa caccgccgct ctgagcggca cgactaccga cgacggctgg cctgaaggta
     2221 gtccccttac cgccacttgg agcgtcgtga gtagccccgg cacggtggcg ttcaacaacc
     2281 cgcatcaggc tgccacgaca gcctccttcg gcgcggcggg caaccggcag cggcgcagca
     2341 atctggacgg cgataggcac gggcagcacg cctgtcaacg gtggcctgct gggagtcttc
     2401 gacccgaccc ttatgctcaa cggcatctat acgctgcggc ttgtcgccac cgacgcagcc
     2461 agccagacgg cgatctcgtc aatcgtcgtg gtggtggacg gccagcggcg ggttcatttc
     2521 aacttactgc gtccaaccca tgaagccgca tatcgtcacc atctcgctgc cgaacgataa
     2581 ggtctacaaa ttcgaggcat atctcgacgc cccgtgcgcc ctcgtcgtcc cgccgcgcga
     2641 ggcgactatt ggtttcagac cgctgcccgg cactaatgcg gccttaactc ccgtggtgcc
     2701 cgtttctgac agagaagctc aacagctttt tggacggcga cttctcgcag gcgtctctga
     2761 acatcgaagg ggcagaccat ccgtcgagtt gacggatggt ctgcccctcc ctctcgataa
     2821 ctacatctac agctctttct ggttcttaca cggtgaatcc aaaccgccgg agcatcagct
     2881 ctacgatgtc acctcctatc gctcatattt atacgaagct tccttattaa ggtgcggtta
     2941 aaccaagccg tcttactgcc agcggaaaac ttttagtgcg acgataaaac tcaagactgc
     3001 ccacgccagc agcacacaga gaggcgtcac attgtcggcc agcgtcgtgc cctcgttcat
     3061 catggcgcgc agcgcatcgt tcaacgcggt gagcggcaga attttgatga agggttggag
     3121 aaaggccgga aaccgctccg aagaaaagaa cgttcccgag agcagccaca tcggcatcat
     3181 caccatattc atcaggccgg acactccttc gagagtcgtg gcgcgtgaag ccagcagcag
     3241 tccgatgccg gaaaacgcga acgcgcctgt gaggagcagc aacacgattg acggaaacga
     3301 gccctgcacc ctgtagctaa acaccaacct tgcaaaaatg atgactgccc cgacttccaa
     3361 caccagtaag atcaggcgtg agaacacgaa tgagagcaga tagtgggaac ggctcatggg
     3421 cgtggcggcc agacgcttga gcagtttccg catgcgcgcc gtcacgatgg caaagccgac
     3481 gcccatcagc ccgctgccca ttatattaag accgagcagc cccggaatca gaaagtcaat
     3541 ataacgcgcg cccgtttcgc tcactctctc ctcgcctgcc gaggttacgt cgcggcgacc
     3601 gagagcgcgc tgcaacgcgt cgtcaaccag aaggcgcgcc gtgcggcttt caggccgcgc
     3661 cgggtcataa cgataattga atggcgcctg cgctgcttgc tccgtcagac cggggacttt
     3721 ctcgttcgcc gctcctgcat caaccggacg caccacgagc gtcactttac cgcttcgtag
     3781 agcgcgcgcc gcatcatcct ttgagagtga gacgacttcg atctcgccgg aacgcgcgag
     3841 cgcagttgcc agctgagaca ccggctcgcc cgccttctgt ctgtcgctct caatcgccac
     3901 acgaatcttt tccggcgccg tattacgaaa ggcgatgccg agcgcaaagg ccagaagcac
     3961 gggaaatatg aagacccaga aaaccgcttc cggctcgcgc acaaattcgc gcaggcgcgc
     4021 gcgtgttaat tcaacgagcg ggtccagccg cacgttcctc ttttcagagg atagcgcggc
     4081 gggcagttcg gctctctccc cggactcgcc cgtatctaca ggttcatttt tcatcttcta
     4141 cattcacgcg ctggtctgtc tgccgcacgc cttcattttt cgcgcaacat tctgcccgtc
     4201 atactgacaa aaacatcttc gagcgtcgcc tgatgcgtcg tcagcgtcag cagttcagcc
     4261 gactcccgtt cgattgcggc cagcaacgcg ggcaacgccg cgctgatatc ggagacgacg
     4321 agcgaacaga cgccgctcat ccgacgcact gcacgcacgc ccgccagagc cgacagcgtt
     4381 tcctctctaa tctcacggct cgcgctaaat tcgacgatct gttccgcgcc gagagaagcg
     4441 acgagtgcgg caggcgtgtc gagcgcgatg atctttccct gatccatgat ggcgacgcgg
     4501 tcgcacagac gcgccgcttc ttccatgtaa tgcgtggtca gaataattgt tccgccgccc
     4561 gcgcgaaact gctcgacgat atcccagagt ttgagccgcg cctgtgggtc gagtcctgtc
     4621 gtcggctcat cgagaaaaag aatgtcggga ttgcccgcca gcgcgcacgc caacgcaagc
     4681 cgctgctttt gccctcccga caacttcccg acgcgcgcgt cgcgcttctt ctccagttcc
     4741 accatcctga ttaactcctc cacgccgcgc ctgtcgtggt aaaaactgcg aaagagtttg
     4801 atagtttctt cgaccgtcag tttttcagcc aacttcgtct cctgaagttg gacgccgaga
     4861 cgttggcgca aatctccggc atgacggtcg ccccagcgat agccgagaac ttcgaccgtg
     4921 ccgccatccg gtttgataag cccttcaaga atttcaaccg tcgtggtctt gcccgcgccg
     4981 ttaggcccca gcagaccgaa gcactcgccg acgcgcactt cgaggtcgag gccgttaacc
     5041 gcagtcacct ctccatatcg tttgcgcaac ccgaccgtgt gtaaagccag cggcacttgc
     5101 ttaaacaccc tctgagcgtc tgtcgtgacc gcgttgtttg aaatcctcat gaatctattt
     5161 tcccactcca acctctcgct tactactctg gcggatgcga aacttcataa catcactctc
     5221 cgaccctctt cccttctcca atgacaacga ctgttcccga ctatctccga acatcagagt
     5281 ttattgacgt gtggcgagga tcaatgtaga gtttgcggct atgaatacgc ccaatgactt
     5341 gattcctctc cgagaagcac gccacctgct cggcgtcagc gataataaag taacttccct
     5401 cgttaaagcc ggactactca agacctatac agacctgctg gacaggcgga agaaactcgt
     5461 gagtcgagcc gaggttgagg gactcaaggt tccgcgcgcg gaggccgcat gaaaacaatc
     5521 gtactggcaa acgctaaagg aggtacgggg aaaaccacat gtgccgtcaa cctttcggcc
     5581 tgcctcgcgc tgcaccacgg gaaacgagtt ctcggcatag acctcgatcc tcaaggtaac
     5641 ttcggcatca gctacggcgt agacccacgg cagttgacgc acaccgccta ccacatgctg
     5701 accgccgaat cgccggacgt gcgcgagtac gtcagagaga tcaagccgaa ccttcatctg
     5761 ataccaaacg ccatcgcgcc cgatctggag aaccggctgg aggccagtca caaccgcgat
     5821 tccctgctcc ggctccggct tcgccagatc aagaatcagt acgattacgt gatcatcgac
     5881 acgcctccgg cgatgcgaac ggccaccatg aacgcgatag tcgccgccga cgaggtgatc
     5941 gtagtcgttg actgcggatt ctacgcgctc tacggtctga ctgacttaat gcgccagata
     6001 gcccgcgtct ctgatgctta tgagaaagat caaatagtga tccggggctt gctcaacatg
     6061 ttcaatcgcg ggcagaatct ggatcgggat gtcaagaagg aggtttcaga cttcttcggc
     6121 ggactgatga tccagaccat tatccataag aatgtgaaac ttgccgaggc cgcctctgcc
     6181 agccaatcta ttattgagtt cgactcaagc gcgcccggag cgttcgactt catcaaacta
     6241 agcaaggagt tgcttgccga atatgagcca gaagaagaaa agcggtctgc aacgcggtct
     6301 aagtgacatc gtagagaaac aaaccgcgcc gacacccgac aaatctcaac ttagccgggg
     6361 actcatcggt aagttcacgg aacccaagcc aaccctgagt actcagggta ccaatagtac
     6421 cccgcgtacc ctgagtaccc acccggctcc gatgcgcgac ttccagaagg tcgctaactc
     6481 catcatgcgc gaggccgtgc cagccggagt gttcaagggc aagtgcaagc agatttatga
     6541 ctttctctat tcaaagactc gtggggcgat tgtcccggct cggtctgcaa aattgacgcg
     6601 gcaagctatc atggaaggtt cgcacgtcgg ctcgacaaag accctctttc tcaatctacg
     6661 gcacctgcgt acagccggac tcgtctcttg gacggagtgc gaaggcacgc atggcggaaa
     6721 cgaatacacg gtctatctgc cggaggaagc cctgagtacc ctgagtactc agggtactct
     6781 gagtgcccct ggttacaatc tacccatggt acccagcgta gaaagtacta tgggtactca
     6841 gggtttatct
//
LOCUS       DASWNS010000344         7713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_39600, whole genome shotgun sequence.
ACCESSION   DASWNS010000344 DASWNS010000000
VERSION     DASWNS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7713
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39600"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VGB76_12000"
     CDS             <1..1132
                     /locus_tag="VGB76_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0099663.1"
                     /translation="VDSYLDDPLCKAVRRVVDAGVVVVVAAGNLGSVEGQKYYGLIES
                     PGNEPSALTVGASNTLGTDSRADDVMTTYSSRGPTRSFRTDENGVAHYDNLIKPDLVA
                     PGNKLVSAAGDDNYFITNYPHLSVYKTTKDEIRMMYLSGTSMATPIVSGAAALLLQAN
                     PRLTPNMVKMLLQYTAQPLAGFNMFEQGAGQVNIEGAVRLARMMRTDLMPDIPLGDPL
                     LATNMLPNPATTLSGYTFPWAQGLIIDRTYATGSELISLNQGIYGNGVLISNGVLISN
                     GVLISNGVLISNGIVLGDQILTSNGYPLGSGDSFCATTSLLADTEGTSQEGLTLGNGV
                     LSDNGVLISNGVLISNGITTSLSYVWADTSIAVNGDPTTCMK"
     gene            1271..2113
                     /locus_tag="VGB76_12005"
     CDS             1271..2113
                     /locus_tag="VGB76_12005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0099664.1"
                     /translation="MENVSLAVTPLDDSRRDEVLAFLNRKPTHTFMMVGLIRDNGLES
                     TLNRGTFYACRDPRGMLAGVALIGEITMIEAHTDAALETLARRTQGVPNIYMIIGEQR
                     QTRLFWDYYAEAGQEMRLCCRELLLELRYPVEVHPPVPGLRPATLAEVEPLMSVHAEM
                     AFEESGVNPLEVDPEGFRRRCARRIEQGRTWVYIKQGRLIFKADVISETPEIIYLEGV
                     YVSPEARGQGYGVRCLSQLSQHLLAHAKSICVLVNERNQAAQALYRKARYKPDGIYDT
                     VFLH"
     gene            2166..4421
                     /locus_tag="VGB76_12010"
     CDS             2166..4421
                     /locus_tag="VGB76_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0099665.1"
                     /translation="MKEVVGKQKYVLPFAALISACGAAACILSAYRLPFARFDAGLLL
                     LALLTILLGSRLTMQLPRAKVHVTVCDTLIFIILLLYGGEAAVLIAAMEACYTSFRFR
                     SQGITIRLDGVLFNTALMACSTFLSAWALRSCLGFDAVSSQVDNGTLFTAVCVMALVQ
                     YAANSSLAAIYTACETNRGIWSTWNNKYFPTSLTYFAGAATATGLVTLKGLIGFYAVI
                     ATATAVTIIYLTLRRYIDDLKASVSQAEQAEHARAEVERARAEQAERHVDELSHYIAE
                     LKRTTDELQSSKEHFRHAAFHDALTNLPNRALFTDHLRRAIEQTKRQPGHHFAVLFMD
                     LDRFKNINDSLGHIYGDQLLVAIAKRLKGCLRQVDVIARLGGDEFAILLDGFTDPNDV
                     LRVAEKIQRVITEPLTIDRNEAFTSASIGIALSSTGYERPEDVLRDADTAMYRAKDGG
                     KARHEVFDEVMYARAVSLLNLENDLRRALERQEFQVYYQPIVTLKTGSLCGFEALVRW
                     QHPTRGMISPAEFIPLAEETGLIIPLGQWVLEESCRQMREWQEQSFANRMLTVSVNLS
                     GKQLSRGDLCQQVKSILERTGFDPRSLKLEITESMVMENADLAIIMLKQLHELGIQLS
                     IDDFGTGYSSLSYLHRFPVNNLKIDRSFISRMGSGDENLEIVRTIIMLARNLGMEVIA
                     EGIETEEQLAQLRALSCGFGQGYLFSVPLNAGSATALIRQVQQPVAALSPTADAPHEG
                     DSKYLSSPLIM"
     gene            5217..6683
                     /locus_tag="VGB76_12015"
     CDS             5217..6683
                     /locus_tag="VGB76_12015"
                     /inference="COORDINATES: protein motif:HMM:NF027787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HEY0099666.1"
                     /translation="MTSSRLTGKFAGLCLGFSILLFCFAAPVTPVAAYTNNDNFANAE
                     TITGNSGSLTVYNDYGTLESGEPSHAGQSIGASIWYQWQATASGNVTFDTAGSHMDTV
                     IGVYTGSSVSALTAVANDDDTGSNLHGSVTFSATQNTVYYIALDAYDWGSYASTMSLN
                     WHLGAATPTPTPDASNRIAFSADRGGATGVYVMDGDGFGQTRLTEKATYNFGAVWSED
                     GTKIAFNSYRDGQSEIYLMNADGSNQTRLTTSAIGSYQPAWSPDGTKVAFRSYDSQAN
                     SDIYVVNTDGTGLTNLSNDPCGCSYTPLWSPDGTQIMFMIEDDYASRVYIMDADGSNQ
                     TQLTNNIEYSAAWSPDGTKIVFVSERDGAPEIYVMNADGSNQTNLTDTYNVLPYAPFN
                     DAPVWSPDGTKIAFATDRDGQVEVYIMDDDGSNLARLTNSIGSDEPRWSADGLKLLFT
                     TIRDGNQEIYSMNPDGSSQVNLTNHPGYDSQAGWQPAP"
     gene            6890..>7713
                     /locus_tag="VGB76_12020"
     CDS             6890..>7713
                     /locus_tag="VGB76_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0099667.1"
                     /translation="MLRIEKAAVLGAGTMGAQIAAHLANAGIPVLLLDIAPRELTPEE
                     QAKGLTLDTRAVRNRIAQGGLEAAKKAKPAAFFAAERAALVSVGNFADDMANLKDCDL
                     VIEAVVENLEIKRRLYAEVEKHRRAGSIIASNTSGIPIRAIAEGFSEEFRAHFLGIHF
                     FNPPRYLHLCELIPTEWTKPEVACSIFGFLDQRLGKGVVIAKDRPNFIANRIGTHGAL
                     VSIKTMLEDGYTIEEVDKMTGQAMGRPKSATFRTFDLVGLDVFAHVAKNLYEAVPE"
BASE COUNT         1621 a         2504 c         2134 g         1454 t
ORIGIN      
        1 cgtcgattcc tacctcgacg atcccttgtg caaggccgtg cgccgtgtgg tggatgcggg
       61 cgtggtggtc gtcgtcgcgg cgggaaatct ggggagcgta gaaggacaga agtattacgg
      121 gctgatcgag tcgcccggca acgagccttc cgcgctcacg gtcggggctt ccaacactct
      181 cggcacagac tcacgcgccg acgatgtcat gaccacctac agttcgcgtg gtccgacgcg
      241 cagcttccgg acggacgaga acggggtcgc acactacgat aacctgatca agccagacct
      301 cgtcgctccc ggcaacaagt tagtctccgc cgcaggcgac gacaactact tcatcaccaa
      361 ctatccacat ctgagcgtct acaagacgac caaagatgag atcaggatga tgtacctgag
      421 cggcacctcg atggcgacgc ccatcgtctc cggcgcagcc gcgctcctct tgcaggccaa
      481 cccccggctt acgccgaaca tggtcaagat gctgctccag tacacggcgc agccgctcgc
      541 cggattcaac atgttcgagc agggagccgg gcaagtgaac atcgagggag ccgtccggct
      601 ggcgagaatg atgcgcacag acttgatgcc cgacatcccg ctcggcgacc cgctgctggc
      661 gacgaacatg ctgccgaacc ccgccaccac tctcagcggc tacaccttcc cgtgggcgca
      721 ggggcttatc atcgaccgca cctacgccac cgggagcgaa ctcatcagcc tcaatcaagg
      781 gatttacggc aacggcgtcc tgatcagtaa cggcgtcctg atcagcaacg gcgtcctcat
      841 cagtaacggc gtcttgatca gtaacggcat cgtgctcggc gaccagatac tgacgagcaa
      901 cggctacccg ctcggcagcg gcgactcctt ctgcgccacc acgtcgctgc tcgcagacac
      961 ggagggcacg agtcaggaag ggctgacgct cggcaacggc gtgctctcag acaacggcgt
     1021 cctgatcagc aacggcgtgc tcatcagtaa cggcatcacg accagtctct cttacgtctg
     1081 ggctgacacg agcattgcgg tcaacggcga cccgacgacc tgcatgaagt agccgacgct
     1141 gctgacgggt caactgcgcg atcactctca cgcctcgttt ctaaaatcga acttcgttaa
     1201 ccgctggggg acacggcaca tgttacgcaa tacggcatta gctggagcga gtacaccatc
     1261 cctgtatggt atggagaacg tttctctggc tgtcactccc ctcgacgaca gccgccggga
     1321 tgaagtgctg gcctttctca accggaaacc cacgcacacc tttatgatgg tcggcctcat
     1381 tcgtgacaac ggattggaga gcacgctcaa ccgtggcacg ttctacgcct gccgcgaccc
     1441 gcgcgggatg ctggcgggcg tcgctctcat cggcgagata acgatgatcg aggcgcatac
     1501 ggacgctgcc ctggagactc tggcgcggcg aacgcagggc gtccccaaca tctacatgat
     1561 catcggagag cagcgtcaga cacgcctctt ctgggactac tatgcggaag ccgggcagga
     1621 gatgcgtctc tgctgccgcg aactgttgtt ggagttgcgc taccccgtcg aagttcaccc
     1681 gcccgtgccc ggcctgcgcc ccgccactct ggccgaagtc gaaccgttga tgagcgtcca
     1741 cgcggagatg gcgtttgagg agagcggcgt caaccctctc gaagttgacc cggaaggttt
     1801 cagacgacgt tgtgcgcgcc gcattgagca ggggcggacg tgggtataca tcaagcaggg
     1861 acgactcatc ttcaaggccg atgtcatctc cgagacgccg gagatcatct atcttgaggg
     1921 cgtctacgtg agtccggaag cgcgcggtca gggctacggg gtgcgctgcc tctcccagtt
     1981 gagccagcac cttctcgcgc acgccaaatc aatctgcgtt ctcgtcaacg agcgcaatca
     2041 ggcggcgcag gcgctctacc gcaaagcccg ttacaaaccg gacggtatct acgacaccgt
     2101 cttcctccac tagacctccg cagacgacgg cgcgcgctcc cccgcgccgt ccctctcaag
     2161 cctcaatgaa agaagttgtg ggcaagcaga aatatgtact gcctttcgcc gcgctcatca
     2221 gcgcgtgcgg ggcggcggcc tgcatcctct cagcctaccg cttacccttc gcccgcttcg
     2281 atgcgggtct tctcctgtta gcgttgctga cgatcctttt aggctcgcgg ctcacgatgc
     2341 agcttccgcg cgcgaaggtg cacgtcacgg tttgcgacac gctcattttc atcatcctgc
     2401 tcctttacgg cggcgaggcg gcagtcctca ttgcggcgat ggaagcctgc tacacctctt
     2461 tcaggttcag gtcgcagggg atcaccatcc ggctcgacgg ggtattattc aacacggcgt
     2521 tgatggcctg ctcgacgttc ctgtcggcct gggcgctccg ctcctgtctg ggtttcgacg
     2581 ccgtctcgtc gcaggttgat aacggcacgc tctttactgc cgtctgcgtc atggcgctcg
     2641 tgcagtatgc cgccaactct tcgctcgccg cgatctatac cgcctgcgag acgaaccggg
     2701 gcatctggtc aacctggaac aataaatatt tcccgacctc tctgacctat tttgccggag
     2761 cggcgaccgc gaccggactc gtcaccctta aaggcttgat cggattctat gccgtcatcg
     2821 ccacggccac cgccgtcacg atcatctacc tcacgctccg caggtacata gatgatctga
     2881 aagcctccgt ctcgcaggcc gagcaggcgg aacatgcgcg cgcagaggtt gaacgcgccc
     2941 gcgccgagca ggccgagcgg cacgtggacg aactcagcca ctacatcgcc gaactgaaac
     3001 ggacgaccga cgaactccag tcgagcaagg aacacttccg tcacgccgcc tttcacgacg
     3061 cgttgacgaa cctgcccaac cgcgccctct tcacagacca cctgagacgc gccatcgagc
     3121 agacgaagcg acagcccgga caccatttcg cggtgctctt catggacctc gaccgcttca
     3181 aaaacatcaa cgacagtctc ggccacatct acggcgacca actgctcgtc gccatcgcca
     3241 agcggctcaa gggatgcctc cggcaagtgg atgtcatcgc ccgcctcggc ggagacgagt
     3301 tcgccatcct cctcgacggc ttcacagacc ccaacgacgt gctccgcgtg gcggagaaaa
     3361 ttcagcgggt aataaccgag ccgctgacga tagatcgcaa cgaagccttc acttccgcca
     3421 gcatcgggat cgcgctcagc agcaccggat acgaacgtcc ggaagatgtg ctgcgcgatg
     3481 ccgacacggc catgtaccgg gctaaagatg gaggcaaagc acgccacgag gtcttcgatg
     3541 aagtgatgta cgcacgcgcc gtgtcgctcc tgaatctgga gaacgacttg cgccgcgccc
     3601 ttgagcgtca ggagtttcag gtctactacc agcccatcgt gacgctcaag accggcagcc
     3661 tctgcggctt cgaggcactg gtgcgctggc aacaccccac gcgcggcatg atctcgcctg
     3721 cggagttcat cccgctcgcg gaggaaaccg gcctcatcat cccgctcgga caatgggtgc
     3781 tcgaagagtc ctgtcgccag atgcgcgaat ggcaggagca atcgtttgcc aaccggatgc
     3841 tgacggtgag cgtcaacctc tccggcaaac agttgtcgcg cggcgacctc tgccagcagg
     3901 tcaagtccat tctggagaga accggcttcg acccgcgttc tctgaaattg gagatcaccg
     3961 agagcatggt gatggagaac gccgacctcg ccatcattat gctcaagcaa ctgcacgagc
     4021 ttggcatcca actgagcata gacgatttcg gcacgggcta ttccagcttg agttatctcc
     4081 atcgcttccc cgtcaacaat ctgaagattg acaggtcttt cattagccgg atggggtctg
     4141 gcgacgagaa ccttgagatt gtccgcacca tcatcatgct cgcacgcaat ctgggcatgg
     4201 aagtgatcgc cgaagggatc gagactgaag aacaactggc gcaactccgc gcgctctcct
     4261 gcggctttgg tcagggctac ctgttttccg tgcccctgaa cgccgggtcg gccactgccc
     4321 tgatacggca ggtccaacag cctgtcgctg ccttatctcc cacggcggac gcgccccatg
     4381 aaggcgacag taaatatctc tccagccctc tcatcatgtg agcgggttct ctggcgatgc
     4441 acgggcggcc ttcgccggag atggtgatgg tcgccgggtg tgccagccct ctcggtcgag
     4501 ctttctcact cgcacttaaa tcgtgtgcag agtgccggac gagaaggcga aaatttagcc
     4561 tccgcgactc ctgaacggcg atagtcgcgg tttcaatctt gcccgtcaga agttaagctc
     4621 aggtcaaact gtgcttaact tcatcacacc atttatctaa tggagagcgc gcaggcgaga
     4681 agttccggac agcaaaactt tgttcttgac ttctaccggc aagcaactag aatcaaagtt
     4741 gtgttcaaga agggacagcg caaattcctg cgaaccttga agcaccgacg ttaccttacc
     4801 tctctctaaa ttcagatcga atcccgccgt cgcgaaagtc agacggtgaa gtgtggcgtg
     4861 aggtgaaagc ctgtcgcgcc tccgtccgca aacggcaacg ccgtcacagc ctgactgcga
     4921 cgccgtttcg tcccgacgac ttctttgcgg cgttacgatc tggaaaacga gaggcgtttc
     4981 gccggtgtgc gtgattacgc gttagtcgca gcccggacgt tcgccgtctc tttcccgcat
     5041 gcccttcgcg tggaagaaac gacgcccgcc ggatgcccgc agagcttttg aaacgtgcgc
     5101 gggcagccgg gttttacgtc acgattttgc gcctgttaaa tcggcgcgcc gctcagtcga
     5161 acctcatccc gacgcggcgc gctcataaat ctcaccacct ataaggagaa aatctgatga
     5221 cttcttcacg cctcaccggc aagttcgccg gactctgtct cggtttctca atcttgttgt
     5281 tctgttttgc agcacccgtc acacccgtcg ccgcttatac caacaacgat aacttcgcca
     5341 acgcggaaac tatcaccggt aactctggaa gcctgaccgt ctataacgac tacgggactt
     5401 tagagtccgg cgaaccgtcc cacgccgggc agtctatcgg agcctccatc tggtaccagt
     5461 ggcaggccac ggcgagcggc aacgtgacct tcgatacggc gggcagccac atggataccg
     5521 tgatcggcgt ctataccggc agcagcgtct cagccttaac cgcagtcgcc aacgacgacg
     5581 acaccggctc caatctacat ggcagcgtca ccttcagcgc gacgcagaac accgtctatt
     5641 acatcgcgct cgatgcgtac gactggggga gctacgccag cacgatgtct ctcaactggc
     5701 atctcggcgc cgcgaccccg acgccgactc ccgacgcctc gaatcgcatc gctttcagcg
     5761 cagatcgcgg gggagcaacc ggcgtctacg tgatggatgg cgacggcttc gggcagacgc
     5821 gcctgacgga gaaagcgacc tacaactttg gcgccgtctg gtctgaggac ggcacgaaga
     5881 tcgccttcaa cagctaccgc gacggccagt cggagatata cctgatgaac gccgatggct
     5941 cgaaccagac ccgtctcacc acctccgcca tcggttccta tcaacctgca tggtcgcccg
     6001 acggcacgaa ggtcgctttc cgcagctacg actcgcaggc caattccgac atctacgtcg
     6061 tgaacacaga cggcaccggc ctcaccaatc tctccaacga cccgtgcggc tgctcctata
     6121 cgcccctctg gtcgcccgac ggcacacaga tcatgttcat gatcgaggac gactacgcct
     6181 cacgcgtcta catcatggac gccgacggct cgaaccagac gcaactcacg aacaatatcg
     6241 aatattcggc ggcatggtcg cccgacggca ccaagatcgt tttcgtcagc gagcgcgacg
     6301 gcgcacccga aatctacgtc atgaacgccg acggctcgaa ccagaccaac ctcacagaca
     6361 cttacaacgt gctcccttac gcgcccttca acgacgcgcc tgtctggtcg cccgacggca
     6421 cgaagatcgc cttcgccacc gaccgcgacg gccaggtcga agtctacata atggacgacg
     6481 acggcagtaa tctcgcgcgc ctcaccaact ccatcggctc tgacgagcca cgctggtccg
     6541 ccgacggttt gaagctcctg ttcaccacca tccgcgacgg caaccaggag atctactcga
     6601 tgaaccctga cggcagcagt caggtcaacc tcacgaacca ccccggctac gatagtcagg
     6661 ccggttggca acctgcgccc tgacaatcag cgcacgagca gcagagatgc acggcgaaat
     6721 cctcactccg cgcctcagcg agaagtccct ccccgagtat taacaacttg agggagggac
     6781 ttcgctcata aggtgagaaa ttattcgcgc gtcaggcttg ctgaatgaat actcattcag
     6841 tatcataggc gtccactcgc acgaactttt taggaaaggt tcttccccta tgcttcggat
     6901 cgaaaaggcg gcggtgctcg gcgcgggcac gatgggcgcg cagatcgccg cgcacttggc
     6961 aaacgccgga atccccgttc tacttttaga catcgcgccg cgcgaactca cgcctgaaga
     7021 acaggcgaag gggctgacgc tcgacacacg cgctgtgcgc aatcgcatcg cgcagggcgg
     7081 actcgaagcg gcgaaaaaag ccaagcccgc tgccttcttt gcagccgaac gcgccgcact
     7141 cgtctcggtc gggaatttcg cggacgacat ggcgaatttg aaagattgcg atctcgtcat
     7201 cgaagccgtg gtcgagaatc tggagatcaa gcgacggctc tacgcggagg tagagaaaca
     7261 caggcgcgcc ggatcaatca tcgcgtcgaa cacgagcggc atcccgatcc gcgccatcgc
     7321 cgaaggcttt tccgaagagt ttcgcgccca ctttctcggc atacactttt tcaatccgcc
     7381 gcgctacctg catctgtgcg aactgatccc gactgaatgg acgaaaccgg aagtcgcctg
     7441 ttccatcttc ggcttcctcg accagcgact cggcaagggc gtcgtcatcg ccaaggatcg
     7501 tccgaacttc atcgccaatc gcatcggcac tcacggcgcg cttgtctcga tcaagacgat
     7561 gctcgaagac ggctacacca tcgaagaggt tgacaagatg acgggtcagg cgatgggtcg
     7621 ccccaagtcc gccacctttc gcacgttcga tctcgtcgga ctggatgtgt tcgcgcacgt
     7681 cgcaaaaaat ctctatgagg ctgtgccgga aga
//
LOCUS       DASWNS010000345        14470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_39634, whole genome shotgun sequence.
ACCESSION   DASWNS010000345 DASWNS010000000
VERSION     DASWNS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14470
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39634"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VGB76_12025"
     CDS             <1..698
                     /locus_tag="VGB76_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099668.1"
                     /translation="RLAALGQMAATVAHEVKNPLSAIKSIAQVMREDERLSAEYTRDL
                     DLIVGETDRLNRSVTQLLNFARHSPTDGTPAPADELARGVVELFRAEAKARNIAVNLR
                     CGEVRRRLDGAQASAVRDALSNLLLNALQETPAGGRVSVEVDTDGGELLFAVTDGGRG
                     VPSELRQRIWEPFFTTKQRGTGLGLAIVRKRLEDAGGAARLAPQAAGAGARFELRLPL
                     AAAAEQTLEAQVY"
     gene            734..2242
                     /locus_tag="VGB76_12030"
     CDS             734..2242
                     /locus_tag="VGB76_12030"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0099669.1"
                     /translation="MTQKRSGDAINLLLVDDEEAARYGMRRALANLGYEIAEAGSVHE
                     ARAQFARRAPELLLLDVNLPGQSGLEFLRELQQQGAEGTPKDAPLVILITAHGSERMA
                     VEAIKSGAYDYLSKPFEVEDLRLVVKNAVETIQLRRENQLLRERIELEGAQRGSLIGN
                     SESMKRVRTLIEKVSETDATVLVRGESGTGKELVAREIHERSASRRRGAFVAVNCAAL
                     PSELIESELFGHEKGAFTGATARRQGKFEQADGGTLFLDEIGDMSANVQAKLLRALEE
                     RRIERLGGGAESIPVDVRIVSATHRPLEQEIEKGNFRADLFYRLRVVTVDITPLRERR
                     EDIPALAESFVRLAAERYKMPVRAIAPAAMRRLVEYEWPGNVRELRNAVERAMVLAEG
                     QELTARDLPEEINAVVHETQSATAPRAATLEAGNDLLVPFTADFRDDRREFERRYITR
                     CLEETGGNVTRAASILGMHRQSLQHKLRELGLARRYVTLDGDATQTPDKSDN"
     gene            2492..3880
                     /locus_tag="VGB76_12035"
     CDS             2492..3880
                     /locus_tag="VGB76_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099670.1"
                     /translation="MKTFTQKFRTVGFFFLLAHLAGLQAQAQELARETKDGNAAVRHL
                     IICVDGVSFSTIEEMRAEGRFKLFRQPARMIAPFPSLTNLALSEILKPAGASEAVGYE
                     DNYFDVENNRMRGGLLDRFRGGRFIKGTFRELFDYHPSAIKSGLGYVAPPASTYLESL
                     TDILRLKQKFRGSREPVFFAYTGASDSLAHLGGKRLLKSFLVRLDETIKDLVREEGGR
                     VEVTIFSDHGNDFEGYRRVSLKSALRRANFRLQKKVKDERSVVLPQFGLVGCAVMWTK
                     EKNEARLAEAVAHVRGVDFAAYEKERVVYVLNERGRATIERRGERFRYRPTQGDPLEL
                     LPLAETLKRQGKGDAEDFIADEDWFAATVESARPDVLRRVYEGVSERALNRANVIVNF
                     ADGYYTGSASLDLFAFLQATHGNLGAQQSFGFVMSSALDLPTHLRARDVWRAIGSPVL
                     KKQQVRNEEASR"
     gene            3994..4650
                     /locus_tag="VGB76_12040"
     CDS             3994..4650
                     /locus_tag="VGB76_12040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEY0099671.1"
                     /translation="MKARLDAILQREQASYLDQLHPASEGLLAEMEAFAGAHRVPIAD
                     REVALFLEITARATGARRVLEIGMAIGYAVIHLLRGMDQNGQVVTIEPSEEMIKRASG
                     YFERAGVSERVRIERGFALDVIPTLEGSFDLVYLDALKEEYADYLELSLPLLRRGGVV
                     IADNLLWGGQVAGEIRAPDQTRSTESLRRFNQQFVNHKNLRAVVLPVGDGLGYGVKVE
                     "
     gene            4739..5941
                     /locus_tag="VGB76_12045"
     CDS             4739..5941
                     /locus_tag="VGB76_12045"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0099672.1"
                     /translation="MQTISVAHTQPARAGVRPEIELMLCCARTRMEAGMVARLRALLD
                     GTLDWDFALRFAERHSLIPLLYFHLHAHAAEKVPPVVYEKLRDQYQRISALNMYLSGE
                     LRRLLKLFAAHEIDAIPYKGPALAAKAYGNIALRHFGDLDILVRQRDVLRVMELLVAE
                     GYELHPPLNGVQQALMLRTQCNLPFTRDGRRLIVEIHWRVSARLFSSPLDEESLWENS
                     NLESFAGTTTRALAPEDLLLSLCVHGAKHLWERVSWIADIAQLLEVYPDLNWPRLLER
                     TRRSGTERMLLLGLQVARDLLGAKLPVEVEAQCQSDPEIAPLAGQIYSRLFAEGNEAS
                     GMSGYFLFQLKARRRLRDKFNYCRYVVSPTEEDLTLLSLPASLSFVYYLLRPLRMLWT
                     GGPSHLQH"
     gene            6405..7367
                     /locus_tag="VGB76_12050"
     CDS             6405..7367
                     /locus_tag="VGB76_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099673.1"
                     /translation="MISKRFVLSLAGTLFFASPVAVNAVQQQPKPKSVTLTEASPSST
                     KKSPPPAAVPTGDVKQRTPAANDASAAQKNAKTPARTAAGTPAANSKPWSVKVTKKAP
                     VSLSLTAKNGRLTEIAAEISQRLKAPVILTPLMQQQSVSQEFEALPLEGALRLLAPQV
                     YIDYELSSDGSQQPKVIGIYLQGMNEPPPAEAAVVKGDSQAILIEGNTEDGLDASTAQ
                     DEEKQPLHVTVEKNQISVRARKQPLTAVLYEIASKVDIPFEMKYESTELVDVDFNNYT
                     MEQAVRTLSPNIRLYQRTNLTNYEVRPLRLVLMPPANNQQTTKM"
     gene            7382..7567
                     /locus_tag="VGB76_12055"
     CDS             7382..7567
                     /locus_tag="VGB76_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099674.1"
                     /translation="MNTKNSNHVNEQLEQGEGVLNQPTQEKKPFVEPVVSVPVDVLEA
                     TAFFQGPTVDTIQTLTT"
     gene            7733..8632
                     /locus_tag="VGB76_12060"
     CDS             7733..8632
                     /locus_tag="VGB76_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099675.1"
                     /translation="MYFTPLAARAARFVHCTIRIFYGDPPTLPPEYQSFEVPRGQCYT
                     NGEEHFLDIDDSRIAVYPPSSRLVCVWLGTTARARHPVAIVNTLSYVVQAALRRGGLY
                     VLHAAGVVEPETGTGLVVVGNSNSGKSSLTIRLASAGWRYLSDDMLVLSGEAGGACEA
                     YALRRIFSVSASSLANCHLSGLEAALGAPANSDPSKRKLEPAITFPGSFVESCRPRVL
                     LFPTLTGEAASRLEKISPGESMARLIRQCPWASYDTGTAREHLRVLGMLAKQSASFSL
                     AAGRDLIENSSNAPELLASCLEH"
     gene            8636..9757
                     /locus_tag="VGB76_12065"
     CDS             8636..9757
                     /locus_tag="VGB76_12065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0099676.1"
                     /translation="MAISSAASTVQHELRHISLELTNICNLHCSYCARDDDALYHAPA
                     NFFSVELLRRIIRSAKQPSCGFNSVSFTGGEVTIHPQFKQIVETVREEGWQFGFVTNG
                     WHFDRVYPTLLANREALHMVAFSLDGATREAHDFWRGEGSFVRVMRAITRCYMKGIPF
                     LIKTGIRRDTIPQLEQFALLAARLGAQAIHFSHLLPTSAGAEDESGLNIEERAHAEQE
                     IGVLSNIFKMKIGVATGYYNLNPAPPCQALEGANCNIDYRGRLTLCCNLSGYRGASAE
                     PDVVADLTQEDFAEGFARLRQVAARQLERRRLALEEIARQSVEPDLYTASPCLYCLQS
                     FGKIPWHKPTAAKSRSLPVLSVSAPASPEPSVYLKQESI"
     gene            9757..10035
                     /locus_tag="VGB76_12070"
     CDS             9757..10035
                     /locus_tag="VGB76_12070"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEY0099677.1"
                     /translation="MSQSIPAPHEHIVFTEFDDREGVLVDLNSKRYYTLNETAMIIWR
                     GLERKRTREEIIRDITDAYDVTPEHAARSLDNLIASLQARKLLPAEGQ"
     gene            10444..14376
                     /locus_tag="VGB76_12075"
     CDS             10444..14376
                     /locus_tag="VGB76_12075"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0099678.1"
                     /translation="MRRQFLALALGASLLAVLLPTRFVSVIPAAQAVSTTFVISQFQT
                     GGGVATDEFVELHNVSGVAQDLNGHRLVYRSSTGTSDATTPLAVWTTSTMVPAGGYYL
                     VAASPGYDGAPVADRTYNSGSISMSGAAAGAGIALRNGAANTGTIVDSVAYGTTTTTT
                     FAETANTSVPPNNDSRARKSSGCQDTDNNANDFEALSPSAPRNSLTATVTCGATALTI
                     SVSDVTMAEGNAGTTTFTFNVNLSAPAPGTVTFDIATADGTAQDDNPATEDNDYVAKS
                     ETGRTITTGATSTTFTVSVNGDTNPEPNETFFVNITNIVGATAGDTQGQGTITNDDVA
                     TRAIFAIQGSNTASPFDGTQVITTGIVTARRSFGASNNGFYIQDPNGDSDLNTSDAIL
                     VFTGSTVPTVAVGDLVQVTGTVDEFESSSTDEPDAVSPPDPKTTTELVAPLTINVLST
                     GNQLPAPLTAAIFDPTAVSRGAQLEKYEHMRVSVASLTVSQPTNNFGEFWGVVTGQPR
                     PFREPGIERGDPVPAADEGPHAGTPPPNVPIFDGNFERIMVNSGSSLVAPGSTRRTQI
                     QVTTGTVVTGIVGPLDYAFDNYQIILDYNVTPGVSGGLTAAVPVPTPVAGEFTVASAN
                     LENFTNNASRLNKASLAIRNVMHTPDVLGVIEVLDQATLTALANKINADAANPSAVNY
                     QAFLAETSATQDIGFLVNTARVTLVGTPATQFAGKTFTYAGETDTLHDRPPYILTVDV
                     PQTGTSDLLRVVVILNHTKSLIAVDSPRPRGTGGTEGGRNREKRRLQAEDIADLIEAR
                     KNENLIMMGDLNAFDFNDGLGDIVGTMKGTPAPPNQVVEPSTDRWSFQLFNSLSRLPA
                     DQQYSLLFEGNAQALDHLLVNQKMRARQSRFAYARYNADFSESFAGDANRPERLSDHD
                     AAVSYFLTGAAQGSGTVLISEFRFNGPNGTADEFIELYNNTDSPINVAGWTVVAPGGT
                     GGGGVLSGLTGILGARGHYLIAPSDYSVSGYPADGDLFTAVPDKPYTGGAFLDDGGAQ
                     LLDADGNVIDSVGFSSATGIGFREGTGLIPAGGVAPTAGDQYSFVRKMTSGIPQDTNN
                     NSADFVLVSTSGAVGATVATLGAPGPETTQSPIQRNAAIKPSLVDNCNGIGFPASGCQ
                     NRVRNGAASNPTNAQYGTLSFRRKFTNTTQEDVYRLRFRVVDITTLNSPGYAPNNGQA
                     DLRVLTSGDETVTPSNPFVPGDISVRGTTLEQPPTQTLGGGLNSTLTVDLAEPLASGE
                     SITVQLLVGVQQNGSFRFFVNVEALTADPTSPPPAIKSPQGKNGARRQ"
BASE COUNT         2958 a         4498 c         4272 g         2742 t
ORIGIN      
        1 agcggctcgc cgcgctcggt cagatggccg ccaccgtcgc gcatgaagtg aagaacccgc
       61 tctcggccat caaatccatt gcgcaggtga tgcgcgaaga tgaacggctc tcggctgagt
      121 acacgcgcga tctcgatctc atcgtggggg aaaccgaccg cctgaaccgc agcgtgaccc
      181 aactgctcaa cttcgcgcgc cactcgccga ccgacggcac gcccgcgccc gcagacgaac
      241 tcgcgcgcgg cgtcgtcgaa ctgtttcgcg ccgaggcgaa ggccagaaat atcgcagtca
      301 atctgcgctg tggcgaagtc cggcggcgac tcgacggcgc gcaggcatcg gcggtgcgcg
      361 atgcgctctc gaatcttttg ttgaacgcgc tgcaagagac gcctgcgggc ggtcgcgtct
      421 cggtcgaagt tgacacggac ggcggcgagt tgctgtttgc ggtgacggac ggcggcagag
      481 gcgtgccgtc ggaactccgt caacgcatct gggagccttt cttcacgacg aagcaacgcg
      541 gcacgggctt gggtcttgcc atcgtgcgca agcggctcga agacgcggga ggcgcggcgc
      601 gccttgcacc gcaggcggcg ggcgcgggcg cgcgtttcga acttcgcctg ccgcttgccg
      661 ccgcagcgga gcagacgttg gaagcgcagg tctattaaca gaacatttaa ccgtcgaagt
      721 taggattgaa accatgacgc aaaaaagatc gggcgacgcc atcaacctgc ttctcgttga
      781 cgatgaagag gccgcgcgct acgggatgcg ccgcgcgctc gccaatctcg gctatgagat
      841 cgcggaggcg ggaagcgtgc atgaagcgcg cgcgcaattt gcacggcgcg cgcccgaact
      901 gttgctcctc gacgttaatt tgcccggaca gagcgggctg gaatttttgc gcgaactaca
      961 acaacagggc gcggaaggca cgccgaaaga tgcgccgctc gtcatcctca tcaccgcgca
     1021 cggctcggag cgcatggccg tggaagccat caagtccggc gcctacgact atctctcgaa
     1081 gcccttcgag gtggaagacc tgcgcctcgt cgtgaagaac gcggtcgaga cgattcaact
     1141 gcgccgcgag aatcaacttt tacgcgagcg catcgaactc gaaggcgcgc agcgcggctc
     1201 gctcatcggc aactccgaat cgatgaaacg cgtgcgcacg ctcatcgaaa aagtctcgga
     1261 gacggatgcc acagtgcttg tgcgcggcga gtcgggcacg ggtaaggagt tggtggcgcg
     1321 tgaaattcac gagcgcagcg cgtcgcggcg tcgcggcgcg ttcgtcgccg tcaactgcgc
     1381 cgcgttgccc tcggagttga tcgagtcgga actcttcggc cacgagaaag gcgcgttcac
     1441 gggtgcgacc gcgcggcggc agggaaagtt cgagcaggcc gacggcggca cgctctttct
     1501 ggacgagatc ggcgacatga gcgcgaacgt gcaggccaaa cttttgcgcg cccttgagga
     1561 gcggcgcatc gagcgtctcg gcggcggcgc ggaatcaatt ccggttgacg tgcgcatcgt
     1621 cagcgcgacg catcgcccgc tcgaacagga gatcgagaaa gggaattttc gcgccgacct
     1681 tttttatcgc ctgcgcgtcg tgacggttga catcacgccg ctacgcgaac ggcgcgaaga
     1741 catccccgcg ctcgcggaat ccttcgtgcg gctcgccgcc gaaagataca agatgcctgt
     1801 gcgcgcgatt gcccctgcgg ccatgcgccg cctcgtcgaa tacgaatggc cggggaacgt
     1861 gcgcgaactg cgcaacgccg tcgagcgcgc gatggtgctc gcagaagggc aggagttgac
     1921 ggcgcgtgat ctgccggaag agatcaacgc cgtagttcat gagactcaga gcgccaccgc
     1981 gccgcgcgcc gcgacgctcg aagcgggcaa cgatctgtta gtccccttca cggcggactt
     2041 ccgcgacgac cggcgcgagt ttgaacgtcg ctacatcaca cgctgtctgg aagagacggg
     2101 cggcaacgtc acgcgcgccg cctccatcct cggcatgcac cgccaatccc tccaacacaa
     2161 actacgcgaa ctcggcctcg cgcgccgcta cgtcacgctc gacggagacg cgacgcagac
     2221 gccggataaa tccgacaact gacgggcgaa acgcggcggg aggcgtcaga cgcgtcgaag
     2281 aaaccactct acgatgaagc gtaagttaag agcttcaccg cagagccgaa ccgcagagat
     2341 gcacagagat ttgaatgatc aactctctgc gttaacgctg cgtttcgctc gcgcgtcgct
     2401 gcgctgaatg tttcccccaa cgctggaaca cggcttgcag tgaagctatc ggaatagctg
     2461 cgcgcgggtc gcaggatcat aagggggctt gatgaaaacg tttactcaaa agtttcgcac
     2521 ggttggattc ttttttctgc tggcgcattt ggccggtcta caggcacagg cgcaggagct
     2581 tgcccgtgag acgaaggatg ggaacgctgc tgtccgtcat ctgatcatct gcgtggacgg
     2641 cgtctccttc tcgacgattg aggagatgcg cgccgaagga cgcttcaaac tctttcgcca
     2701 gcccgcgcgg atgatcgctc cgtttccgag tctcaccaat ctcgcgctca gcgaaatatt
     2761 gaaacccgcc ggagcgagcg aagccgtcgg ctacgaagac aactatttcg acgtggaaaa
     2821 taaccggatg cgcggcggac tcctcgaccg ttttcgcggc gggcgattca tcaaaggcac
     2881 ctttcgcgaa ctcttcgatt atcatccttc cgccatcaag agcggcctcg gctacgtcgc
     2941 cccgcccgcg agcacctacc ttgaatctct gaccgacatc ctgcgtctga aacagaagtt
     3001 tcgcggctcg cgcgagccgg tctttttcgc ttacacgggc gcgagcgatt cgctggcgca
     3061 tctgggcggc aaacgcctgc tcaagagttt tctcgtccgt ctcgacgaaa cgatcaaaga
     3121 cctcgtgcgt gaagaaggcg ggcgcgtcga agtgacgatc ttctcggatc acggaaacga
     3181 ctttgaaggc taccggcgcg tctctctcaa gtcggccttg cgccgcgcga atttcaggtt
     3241 gcagaagaag gtgaaggatg aacgcagcgt cgtgttgccg cagtttggcc tcgtcggctg
     3301 cgccgtgatg tggacgaaag agaagaacga ggcgcgtctg gccgaagccg tcgcgcacgt
     3361 gcgcggagtt gatttcgcgg cttacgaaaa agagcgcgtc gtttacgtgc tcaacgaacg
     3421 cggcagggcg acgattgaac ggcgcggcga gcgttttcgt tatcgtccta cacagggcga
     3481 cccgctcgaa cttttgcctc tggccgaaac tttgaagcgg cagggaaagg gcgacgccga
     3541 agatttcatc gctgacgaag attggtttgc ggcgacggtt gagagcgcgc gcccggatgt
     3601 gttgcgacgc gtgtacgaag gcgtgagcga gcgcgctctg aatcgcgcca atgtcatcgt
     3661 gaattttgcc gacggttatt acacgggcag cgcgtcgctc gatctcttcg cctttctgca
     3721 agcgacgcat gggaacctcg gcgcgcagca gagtttcggc ttcgtgatga gcagcgcgct
     3781 cgatcttccg acgcatctgc gcgcccgcga cgtgtggcgc gcgattggct cgcccgtcct
     3841 taaaaaacaa caggtcagga atgaagaagc gtcacgttga tgtaaagccc gcctgtccgt
     3901 gtcacaaacg cctgcctgat tgcgtcggag cgcgcagtac ggttagactt accgcgctct
     3961 tgaaaagatt ccggcggtga tgagttaacg tttatgaaag cacgcctcga cgcgatcctc
     4021 caacgcgagc aagcctccta tctcgaccaa cttcatcctg cgagcgaggg tttgctcgcc
     4081 gagatggagg cgtttgcagg ggcgcacagg gtgccgattg ccgaccgcga ggtggctctg
     4141 tttctggaga tcacggcgcg agccacgggc gcgcggcgcg tgttggagat cggcatggca
     4201 atcggctacg ccgtcatcca tctgctgcgc gggatggatc agaacgggca ggtcgtgacg
     4261 attgaaccga gcgaggagat gattaagcgt gcgagcggtt atttcgagcg cgcgggcgtg
     4321 tcggagcgcg tgcgcatcga gcgcgggttt gcactcgacg taatcccgac ccttgaaggc
     4381 agcttcgacc tcgtctattt agacgccttg aaagaggagt atgcggatta cctcgaactc
     4441 tccctgccat tgctgcgcag ggggggcgtc gtgatcgcag acaatctgct ctggggcggg
     4501 caggtggcag gcgagattcg cgcgcccgac cagacaagat caacggagag cttgcgtcgc
     4561 ttcaatcaac agttcgtgaa ccataaaaac ttgcgcgccg tagtgctgcc cgtcggcgac
     4621 ggcctcggtt acggcgtgaa agtcgaataa gcgactatca gtgtcgcttg cgcccgcccg
     4681 acacgtagac cgcagttaaa ccggaaagtc gcccgcgctc attcttgacc cgttagcaat
     4741 gcaaacaatt tccgtcgccc atacgcaacc ggcgcgagcc ggggtgcggc cagagatcga
     4801 gttgatgttg tgctgcgcgc gcacgcggat ggaggctggc atggtggcgc gtctgcgcgc
     4861 gttgctcgat gggacgctcg attgggattt cgccttgcgc ttcgccgagc gtcacagctt
     4921 gattccgttg ctctactttc acctgcacgc gcacgctgcg gaaaaagtcc cgcccgtcgt
     4981 ctatgaaaaa ctgcgtgatc agtatcaacg catctccgcg ctgaacatgt atctgagcgg
     5041 agaactgcgc cgcctgctca aactcttcgc ggcgcatgag attgacgcca tcccctataa
     5101 aggacccgcg ctcgcggcca aagcctacgg caacatcgcg ctcagacatt tcggcgatct
     5161 ggatattctc gtccgccagc gcgacgtgct gcgcgtgatg gagctgctcg tggcggaggg
     5221 ctacgagttg cacccgcctc tgaacggcgt gcagcaggcg ttgatgctgc gcacgcaatg
     5281 caacctgcct ttcacgcgtg atgggcgtcg cttgatcgtg gagattcact ggcgcgtgag
     5341 cgcgcgtctt ttctcttcgc cgctggacga ggagagtttg tgggagaaca gcaacctcga
     5401 aagttttgca gggacgacga cgcgtgcgct cgcgccggag gatttgctgc tctcgctctg
     5461 cgtgcacggg gcgaagcatt tgtgggagcg cgtcagttgg atcgccgaca tcgcccaact
     5521 gctcgaagtt tatccggact tgaattggcc gcgcctgctc gaacgcacgc gccggagcgg
     5581 cacggagcgc atgttgctgc tcggcctgca ggtcgcgcgc gatctgctcg gcgcaaaact
     5641 tcctgtcgag gtcgaagcgc aatgtcagag cgatccggag atcgcacctc tggccgggca
     5701 aatttattcg cgcctgtttg cggaagggaa cgaggcgagc ggcatgtcgg gttattttct
     5761 tttccaactc aaggcacgtc gccgcctgcg cgacaagttc aactattgcc gttacgtcgt
     5821 cagcccgacg gaggaagacc tcaccctgct ctcgctgccc gcctcgctct ctttcgtcta
     5881 ctatctgctc cgccccttgc gcatgctctg gacgggagga ccaagccacc tgcaacatta
     5941 agcgcgcttt gacgagactg cttctccttt cccctacgcc ttgtgcgaga actcaatcgc
     6001 acgcgggagg cgcaaagaaa gagaggccgc gccggggtgt gatttggagt ttataactaa
     6061 gctccgggtg gtgtggctgc catgccggat tgatgcaggc gggcaaatat gctaagttcc
     6121 gtctgcccgc gcaagtttct ttgacttatc cgatagtcgt ggttgccttc tactaatcaa
     6181 aagccagcct atattgcacg ccagagagcc tgcccgccgg ttgctgcgcc ggagttgtta
     6241 gtggactttt gacgtttaga agatcgaaca gttcagcttt gaactcagct tgcaactaat
     6301 ttcccccgac gacatcgaag tctaagcaca caagccaacc cgacgcttgc tgtttaagaa
     6361 ttaattaatg gatcaggatt aagaggtagg ttaatatgcc cgatatgatt tctaagcgtt
     6421 ttgttctgtc gctcgcggga actctgtttt tcgcgtcgcc cgtcgccgtc aacgcagtcc
     6481 agcaacaacc gaaaccgaaa tccgtcacct taacggaggc gtcgccttca agcactaaaa
     6541 agagtccgcc gcccgccgcc gtgcccacgg gagacgttaa acagagaacg cctgcggcaa
     6601 acgacgcttc cgccgcgcag aaaaatgcta aaactccggc acgcaccgca gcgggcacgc
     6661 ctgcggcgaa tagcaagccc tggagcgtca aagtcacgaa gaaagcaccc gtctcgctaa
     6721 gtcttactgc gaagaacggt cggctgacgg agatcgccgc cgagatcagc cagcgactca
     6781 aagctcccgt catcctgacg ccgctcatgc aacagcagag cgtctcgcag gagtttgaag
     6841 ccttgccgct cgaaggtgcg ttgcgtttgc tcgcgccgca agtttatatt gactatgaac
     6901 tgagcagtga tggcagccaa cagcccaaag tgatcggcat ttacttgcag ggcatgaacg
     6961 aaccgccgcc cgccgaggcc gccgtggtca aaggcgattc gcaggccatc ctgatcgaag
     7021 ggaacacgga agacgggctt gatgcttcaa ccgcgcagga tgaagagaaa cagccgctcc
     7081 acgtgacggt cgaaaaaaat cagatcagcg tgcgcgcacg caagcaaccg ctgacggcgg
     7141 tgctctacga aatcgcaagt aaagttgaca ttcccttcga gatgaaatat gaatctacgg
     7201 aacttgtgga tgtggacttc aataattaca cgatggagca agcggtgcgc accctgtcgc
     7261 ccaacatccg cctttaccag cgcaccaatc taacgaacta cgaagtcaga ccgctgcgcc
     7321 ttgttttaat gccgccagcc aataatcagc aaaccacaaa aatgtgagga gacatcgagc
     7381 cttgaataca aaaaacagca atcatgttaa tgagcagttg gaacagggcg agggcgtgct
     7441 caatcagccc acgcaggaaa agaagccgtt tgtcgagccg gtggtttccg tgccggtcga
     7501 tgtgttggaa gcaaccgctt tctttcaagg cccgaccgtt gacaccatcc agactttgac
     7561 cacgtaatta gctttctccg tgagtgaaag tagccgggag acgatgtctg ttcttgctgc
     7621 gccgaagcgt ttagccccga tcgagaagtt ttacgcgctt ggggatcgcc tcctcgcttt
     7681 tcactttgct gatgaagtag ccgcgcgggt tgccgaagag ttcatcgggg gaatgtattt
     7741 cactccgttg gcggctcgcg cggctcgctt tgttcattgc accatcagga ttttctacgg
     7801 cgacccgccg acactgccgc ccgagtacca atctttcgaa gttccgcgcg ggcaatgcta
     7861 caccaacggg gaagagcatt ttcttgacat tgacgattcg cggatcgccg tctatccgcc
     7921 gtcgtcacgt ctggtctgcg tctggttggg gacgacggcg cgggcgcgcc atcccgtcgc
     7981 catcgtcaac acgctctctt acgtggtgca ggccgcactc aggcgcggcg gactctacgt
     8041 gctgcacgcg gcgggagtgg tggagccgga gacgggcacg gggctggtgg tggtcggaaa
     8101 ctccaacagc ggaaagtcga gtctgacgat ccggctggcg agcgcgggct ggcggtattt
     8161 gtcagacgac atgctcgtgc tctcgggcga ggcgggcggt gcgtgcgagg cttatgcgct
     8221 gcgacgtatc ttttccgtct cggccagttc gctcgccaac tgtcacttga gcgggttgga
     8281 ggccgcgctc ggcgcgccgg ccaactccga tccgagcaag cgtaaactcg aaccggccat
     8341 cacgtttccc ggcagcttcg ttgagagttg ccgcccgcgt gtgctactgt tccccacttt
     8401 aacgggcgag gccgcaagcc gtctggaaaa aatctctccc ggtgagtcga tggcgcggct
     8461 catccgacaa tgcccttggg caagttatga caccggcact gcgcgcgagc atctgcgcgt
     8521 gcttggaatg ttggcgaagc aaagcgcctc tttttcactc gctgcggggc gcgatttgat
     8581 cgaaaattcg tcgaacgcgc cggaacttct ggcttcgtgc ctcgaacact aatttatggc
     8641 aatctcttcg gcggccagta ccgtccagca cgaactcagg cacatcagcc ttgaactgac
     8701 gaatatctgt aatctgcatt gcagttattg cgcccgcgac gacgacgcgc tctatcacgc
     8761 gcccgccaat ttcttctccg tcgaactcct gcgccgcatc atccgcagcg cgaagcaacc
     8821 ttcctgcggc ttcaattccg tctccttcac gggcggggaa gtgacgatcc acccgcagtt
     8881 caaacagatc gtggagacgg tgcgggaaga gggctggcag ttcgggttcg tcaccaacgg
     8941 ctggcacttt gatcgcgtct acccgacgct gctggcgaac cgcgaagccc tgcacatggt
     9001 cgcgttcagc ctcgacggcg caacccgcga ggcgcacgac ttctggcgcg gcgaaggatc
     9061 gtttgtgcgc gtcatgcgcg ccatcacgcg ctgctacatg aagggcatcc cgtttttgat
     9121 caagacgggc atccgccgcg acaccatccc ccaactcgaa cagttcgcgc tcctcgccgc
     9181 acgcctcggc gcgcaggcca tacacttctc ccacctgctg ccgacatccg ccggagcgga
     9241 agacgagtcg ggcttaaaca tcgaagagcg cgcacacgcc gaacaggaga tcggcgtgct
     9301 ctccaacatc ttcaagatga agatcggggt cgccaccggc tactataatt tgaaccccgc
     9361 gccgccctgc caggcgctcg aaggcgcaaa ctgcaatatt gattatcgcg gacggctgac
     9421 tctgtgctgc aacctctccg gctatcgtgg cgcatctgcg gagccggatg tcgtcgccga
     9481 tttaactcag gaagatttcg cagaaggttt tgcgcgcctg cgacaggtgg ccgcgcgtca
     9541 actcgaacga cgcaggctcg cgctcgaaga gatcgcacgg caatccgtcg agcctgatct
     9601 ctacacggcc tctccctgtc tctactgcct gcaaagtttc ggtaagattc cgtggcacaa
     9661 gcccacggca gcgaagtcgc gttcgttgcc cgtgctctct gtcagcgcgc ccgcgtcgcc
     9721 ggaaccgtca gtctatttaa aacaggaatc catttaatgt cgcaaagtat tcccgccccg
     9781 cacgagcata ttgtcttcac agagtttgac gaccgtgaag gcgtgctcgt cgatctcaac
     9841 tcgaaacgtt attacacgct caacgagacg gccatgatca tctggcgcgg actcgaacgc
     9901 aaacgcacgc gcgaagagat catccgcgac atcacggacg cctacgacgt gacgcccgaa
     9961 cacgccgctc gctcgctcga taacctcatc gcatctctac aggcgcgcaa actgcttccg
    10021 gctgaagggc agtgaagtta agttttcgtt aacgatgctc gcttccaatt gtcgcccttt
    10081 cgcttttgtg ttaatttgct cattcagcgc gcaagcaagg gacaagcgaa gggaatcacc
    10141 agcattaagc gtctctgacg ctacagccac gccgccgctt tccaactgca atgtcgttct
    10201 gtgtcactgg tgcgcgtgac accactcaag ccaattcgga agaaatagga tcgtgacctc
    10261 ggttcatcta gcgggcggac tgcggaaact tgatgcccgc cgggtcgtct cgtgagaatt
    10321 taaagccgcc tcagtcaaca agcgtctcaa gtcggacatg gagagacgcc acacctcgtg
    10381 tcatacaaga aaggaatctt tccagtgttt acatcgcctc ttcgccgacg ctccactctt
    10441 cttgtgcgtc gacagtttct cgcgctcgcg ctcggcgcat cgctcttagc cgtgctactt
    10501 cccacgcgct tcgtttccgt catccccgcc gcgcaggccg tctcgacaac gtttgtgatt
    10561 agccagtttc aaaccggcgg cggcgtggcg actgacgaat ttgtcgaatt gcacaacgtc
    10621 agcggtgtcg cacaggattt gaacggtcat cgtctggtgt atcgctcttc gaccggcacg
    10681 agcgacgcga ccactccttt agcggtatgg acgacgagca cgatggttcc ggcgggcggt
    10741 tattacctgg ttgccgcatc tccgggctac gacggcgcac ccgtcgccga ccgaacctat
    10801 aattcgggca gcatttccat gtcgggcgca gccgcaggcg caggcatcgc cttgcgcaac
    10861 ggcgcggcta acacggggac gattgttgac tcggtcgctt acggcaccac gacgaccacc
    10921 actttcgcgg aaacggcgaa tacatccgtg ccgccaaaca atgacagccg cgcgcgcaag
    10981 tcgtccggtt gtcaggacac ggataacaac gcgaacgact tcgaggcgct ctctccctct
    11041 gccccgcgca acagcctgac ggcgaccgtt acctgcgggg cgaccgcgct gactatctcg
    11101 gtcagcgacg taacgatggc cgaaggcaac gccggtacga ccacttttac cttcaacgtg
    11161 aatttgtccg cgcccgcgcc gggcaccgtc actttcgaca tcgccaccgc cgacggcacg
    11221 gcgcaggacg ataatcccgc gaccgaagat aacgactacg tggcgaagtc cgaaaccggc
    11281 agaactatca cgacgggtgc gacgagcacc accttcaccg tctccgtcaa cggcgacacc
    11341 aaccccgaac cgaatgaaac cttcttcgtc aatatcacaa acatcgtggg cgcaactgcc
    11401 ggagacacgc aggggcaggg cacgatcacg aacgacgacg tggcgaccag agccatcttt
    11461 gcgattcagg gaagcaacac tgcttcgcct ttcgacggca cgcaagtcat caccaccggc
    11521 atcgtcaccg cgcgcaggag cttcggcgcg agcaataacg gtttctacat tcaagacccg
    11581 aacggcgaca gcgatctgaa tacatccgac gccattctgg tctttaccgg aagcaccgtc
    11641 ccgacagtcg ccgtgggcga cctggttcaa gttaccggaa cggttgacga gtttgaatcg
    11701 tcatcgacgg acgagccgga cgccgtctcg ccgccagacc ccaagaccac gacggaactc
    11761 gtcgcgccgc tcacgatcaa cgtcctttca accggcaacc aactgcccgc ccctctgacc
    11821 gccgccatct tcgacccgac cgccgttagt cgcggcgcgc agttggagaa atatgagcac
    11881 atgcgcgtca gcgtcgcatc gctcacggtg tcacagccga cgaataattt cggtgaattc
    11941 tggggagttg taacggggca gccgcgaccc ttccgcgagc cgggcatcga acggggcgac
    12001 cccgttcccg ccgcagatga aggcccgcac gcgggcacgc cgccgccgaa cgtgcccatc
    12061 ttcgacggca acttcgagcg catcatggtc aacagcggct cgtctctcgt cgcgccgggc
    12121 agcacgcgcc gcacgcagat tcaggtgacg accggaacag tcgtcacggg catcgttggc
    12181 cccctcgact acgccttcga caactaccag atcatactcg actataacgt cacgcccgga
    12241 gttagcggag gtctgacggc ggcggttccc gtcccgacac ccgtggcggg cgagttcacg
    12301 gtcgcttcgg ccaaccttga gaacttcacc aacaacgcgt ctcgtctcaa caaagcctcg
    12361 ctcgccatcc gcaacgtgat gcatacgccc gacgtgctcg gcgtcatcga ggtcttagac
    12421 caagccaccc tgaccgcgtt ggcgaacaaa attaacgccg acgccgccaa tccgagcgcg
    12481 gtcaattatc aggcgtttct cgccgagaca tcggccacgc aggacatcgg tttcctcgtc
    12541 aacaccgcgc gcgtcacgct cgtcgggaca cccgcgacgc aattcgccgg caagacgttt
    12601 acctacgcgg gcgagacaga cacgctgcac gaccgtccgc cctacatcct gacggttgac
    12661 gtgccgcaaa ccggcacgtc cgatctcctg agagtggtgg tgattctcaa tcacaccaaa
    12721 tctctcatcg cggtcgatag cccgaggccg cgcggcacgg gcggcacgga aggcgggcgc
    12781 aatcgcgaga agcgacgcct gcaagccgag gacatcgccg acttgatcga ggcgcgtaaa
    12841 aacgaaaacc tgatcatgat gggcgacctc aatgcgttcg acttcaatga cggcctcggc
    12901 gacattgtcg ggacgatgaa gggcacgccc gccccgccca atcaggtggt cgagccgagc
    12961 accgaccgct ggtcgttcca actcttcaac tcgctcagcc gccttcccgc agaccagcaa
    13021 tactcgctgc tgttcgaggg caatgcgcag gcgttggatc atctgctcgt gaaccagaag
    13081 atgcgtgcgc gccagtcgcg ctttgcctac gcgcgttata acgcggactt ctccgaatct
    13141 ttcgccggcg acgcgaaccg acccgaaagg ctctccgacc acgatgcggc tgtctcctac
    13201 ttcctgaccg gagcggcgca agggtctggg acagtcctga tcagcgagtt tcgtttcaat
    13261 ggtccgaacg gcacggcaga tgaattcata gagctttaca acaacaccga ttcacctatc
    13321 aacgtggcgg gctggacggt cgttgcgccc ggcggcacgg gcggcggcgg cgttctgagc
    13381 ggcctcacgg ggattctggg cgcgcgcggc cattatctga ttgcgccgag cgactacagc
    13441 gtctccggtt atcccgccga cggcgatctc ttcaccgctg tgccggataa gccttatacg
    13501 ggcggcgcgt tcctcgatga cgggggcgca caactgctcg acgccgacgg taatgtcata
    13561 gactcggtcg ggttcagttc ggccaccggg atcggtttcc gtgagggaac cggattgatt
    13621 ccggcgggcg gggtcgcacc cacggcaggc gatcaataca gcttcgtgcg caagatgacg
    13681 agcggcattc cgcaggatac caataacaac agcgcagact tcgtgctcgt ttcgacaagc
    13741 ggcgcagtcg gcgcaacggt cgccacgctc ggcgcgccgg gaccggagac tacgcagagt
    13801 cccatccagc gcaacgccgc catcaagccc tcgctcgtag acaactgcaa cggcatcggc
    13861 ttccccgcat ccggttgcca gaaccgcgtg cgcaacggcg cggcgtccaa cccgacgaac
    13921 gcgcaatacg gcacgctctc cttccgtcgt aaattcacca acacgactca ggaggatgtc
    13981 tatcgcctgc gcttccgtgt cgttgacatc acgacgctca acagccccgg ttacgcgccc
    14041 aacaacgggc aggccgacct gcgcgtgctc acttcgggag atgaaaccgt caccccgtcg
    14101 aatcccttcg ttcccggcga cataagcgtg cggggaacta ctctggagca gccgcccacg
    14161 caaactctgg gcggtggcct aaattcgacc ctcacggttg atctggccga gcctctggcg
    14221 agcggcgaga gtatcactgt gcagttgctc gtcggcgtgc agcagaacgg cagcttccgc
    14281 ttcttcgtca acgtcgaagc tctcaccgct gacccgactt cgccgccgcc tgcgatcaaa
    14341 tcgccgcagg gtaagaacgg cgcgagaagg cagtaacgtc gagaggcgtt acgaactacc
    14401 tgcctcgctt cgacaaaacg cggctttgac taacagcccc tccgcgatca agccccgagt
    14461 caatgaaaac
//
LOCUS       DASWNS010000346        14265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_91632, whole genome shotgun sequence.
ACCESSION   DASWNS010000346 DASWNS010000000
VERSION     DASWNS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14265
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91632"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2151)
                     /locus_tag="VGB76_12080"
     CDS             complement(<1..2151)
                     /locus_tag="VGB76_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0099679.1"
                     /translation="MSTQEKPKVKKENEYNYTNKFNKKQIQFSPKADEVVATFQTPPT
                     ESMARDAMNASTLSISQGINYERGFAVFQAAPDQDADRAAETLSDQPQIANAIPALID
                     DEGKTRYFLPDEFTVQFRDEISKAQAEKIIQQHGCRVVVVQRTPGYYTLAVPEGKGLF
                     ETLREFSALPEVAFAEPSEAGFNDALSYIPDDPDFPKLWGLHNTGQVVNGIAGSADAD
                     IDATEAWDITRGHPDVIMAVIDTGADMNHPDLLPNFLPRGAEDWDFADGPDQVPDDVD
                     GHGTHVAGTTAAVDNATGVVGVAPKCRIMPLRVNLTSGMNQNRADAINYVAAQAVANP
                     ARRYVINCSWKMNGDHAGVHNAIINAKNKNVVVVFAAGNANTDINVTPQYPAIYPEVI
                     AVVATDQKDKRATFSNFGAATDVSAPGVNIFSSVPNDTFGFNNGTSMASPHVAGLAGL
                     IWSKNRTLTNQQVRNIIESTCDNIDALNPGFAGKLGKGRINAFKALNATPLPPVGFQV
                     LRKFPFPQTNSGSSSGLAFARFVFISPAFRPALLFLTQQPSTEKIFFLNPLTGAVLKS
                     VDPVANDTIGSLEWDGSNIRVANVTVGAGSINTINPNTGAQTATIPTPPGRGEGLAYD
                     GTFFYYSTVTRIHVIKAVAPFNVVRSFIPPGGGTCRALASGKGFLFCGTSTGTVVIFD
                     AVTLAIRGIVPAPGGGAAKVEGLAFNPLTDELFIA"
     gene            2811..4682
                     /locus_tag="VGB76_12085"
     CDS             2811..4682
                     /locus_tag="VGB76_12085"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0099680.1"
                     /translation="MKPKIVAIAGAAKGAIFELGDDDLVIGREGTNHVFLNDKTVSRQ
                     HCIIKKEKALYKINDLESHNGTLINGVPVKEQSLRHGDHIQVGDNFFLFLLHEESGAP
                     VANDVSFDDGTVFNTSSVQFSLEDALYSMARDLNLLIKLGEIVNTIQSLKALQRRLLE
                     SIFEAIPAERGAILLIDESLENPASVFAMDRVRGPAHPVHVSRTVARLVLSEGRAVLS
                     NNVVESDTLAKSESLTAAKIHSLLCVPLTLHKRITGIIYLETSESGAVLNESHLRVLS
                     AIASFVSGVLDNMRHVEWLESENRRLQGDIHAERNIVGESARMQEIFQFIAKVAPTDS
                     TVLLRGESGTGKELVARAIHRTSTRADKPFVAVNCAALTETLLESELFGHEKGSFTGA
                     YAQKKGKFEIAEGGTIFLDEVGEMSPPAQTKLLRVLQEREFERVGGTRLIKANVRVVA
                     ATNKSLEKAIENGAFRQDLYYRLNVISRVMPPLRERGEDIPLLASYFVIKHSAKCKRR
                     VTGLSTQARAILVGYHWPGNVRELENAIEQAVVLGSMDRVQPEDLPEHILEASGAPGE
                     GGMNYHEAVREAKKQLLQKALNQAGGSYTEAAKLLGIHPNNLHRLVRNVGSKTTPEK"
     gene            complement(4742..7693)
                     /locus_tag="VGB76_12090"
     CDS             complement(4742..7693)
                     /locus_tag="VGB76_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0099681.1"
                     /translation="MPFANGTRIGRYVIQSLLGSGGMGEVYLARDLKLERSVALKVLH
                     AVAQADPRAVQRFFKEARAASALSHPNVAYIYEVEEAEGVNFIALEYVQGETLRARLR
                     KSRMSLSDALDVALQVCGALAAAHERGIVHRDIKPENIMVTGEGHVKVLDFGLAKVSE
                     ATPHEEEPNNSTVSRAFSEPGVMMGTVAYMSPEQARGLTMDARTDIWSLGVVFHEMIA
                     GRTPFEGASNSDIIAAILHQPPLPLARFSREVTEAVEWVALKALAKDRAERYETAREM
                     HIDLRRLKRRLETGAEGERTSLEALNSGGASVPGGSWPPQRASSTESSPKDAPPASPS
                     STHSMSSAEYIVSTLHRHRKVSVAAILCFVAAATLGVYLLTAARTAAPAAPPQRLILN
                     RLTFEQGLQGSPTWSPDGQFIAYSSDRDGNFDIWVKSASSGDPLRITDSPEHDWQPNW
                     SPDGTLIVFRSERDGGGLYFTSAPFGKRVRKISSFGYNPRWSPDGSRILFLRQGMRLN
                     DRPRVYVLDKDGASAPHEVLTSPMGDERGVRRGALAWHPDSRRVSYWGEDRAFYTIPI
                     EGGTPVKSEVSEEVAARMRKAAIDWGAFQWSPSGASLYLEGRSNGVLNLWRIQVDPKS
                     MQWTGGPERLTTSDGQDTEIALARDGRKLAYTKKSQGTSLWLLPFDARRGLVKGEGHP
                     VTPKDVEAWFPDISPDGRKLVYSVIRQGQTKHEIWEKSLVNSEQRLLSEVEGAVPFNP
                     RWSPDSTRIAYSRHELIPPRRIKRGGSVVILDSASGAEQTMASHPPAGEASPWRDYNY
                     DWSPDGQWVLVSSDRGSPERWYIAMLPLDGAPNAELKLRKVLEEANVNLWTPRFSPDG
                     RWITFLKQDPNETASSVLYVAPASGGAPVRVTDEKAWADKPRWSPDGRTLYFIYNQNS
                     YFLNVYGIRFDPEQGRTVGEPFRVTNLGSPARMVAASLSYLEISLNRDFLVLPITETN
                     GNIWVLEDLIP"
     gene            complement(7929..8768)
                     /locus_tag="VGB76_12095"
     CDS             complement(7929..8768)
                     /locus_tag="VGB76_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099682.1"
                     /translation="MSEYRVVVSAENNPFMAWQCKLFHFSCVSRLRLTPTFIVHESGK
                     DWHPDFRDIVRAGGIVRHAPSYALTPQGQRYSPRNTPGTLLHAAELYRGTDDLIVLCD
                     PDMLFVNEPKFPRMLSGEYYPYMRYDQEAVQTARQKLGVAACEIESRGSELCLGVPHV
                     VPSADAQRLAETWLEAIDAFPPVLWEISMYALGLAAVKLKMRIELTYMLGFSHRMDAP
                     IVWDMIHYCYGDERWSKHHFHYDEQARAVWKPQVDAPGGTVLGEILAQLREAGEFYGR
                     FTF"
     gene            complement(8790..9395)
                     /locus_tag="VGB76_12100"
     CDS             complement(8790..9395)
                     /locus_tag="VGB76_12100"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY0099683.1"
                     /translation="MKKAVLEAAPLEYTEEVAGIISTQLYSPRYCRGLVEYARAADGW
                     TAASIFERGRGAGDAAARPAYRSARALAFDSRSPVGREFDEQMARIVRPLVNRVWQAN
                     LETHKATHIVRYAPGDFYVAHTDTIPGDDYRYFTVLCYLNEEFEGGQTTFPALNYSFK
                     PRTGAAIIFPATYLHCAEPVTAGEKYAVVTWLCGAPPVRWI"
     gene            complement(9999..11153)
                     /locus_tag="VGB76_12105"
     CDS             complement(9999..11153)
                     /locus_tag="VGB76_12105"
                     /inference="COORDINATES: protein motif:HMM:NF036911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamine glutaminase family protein"
                     /protein_id="HEY0099684.1"
                     /translation="MGALQAKVGKVREMRRRGDGAPLLEVAKEAGRAALDDDIEIVIG
                     DEGDESVFRLIRTNPRFGIWVEYLESRHKSGIPVYVEADTETGEVYEVIPTYLYTVEF
                     IETAADGSGLEVSFFQSPAVSPLRGSHPKFEELRKILKDAWEDHSRVLVTCTTFKREI
                     VDVRPAPVDLNLPGLANGPVDVLEMPLLKDVLPFAFRELLPASFISHLTISQALEAFD
                     FVAAIPELQIPFRYPTDCCMARAHLMCRRLLEQRGITARKIWNYGTGLVNNYPNNRAT
                     LRFVTDNDPEGYVLWRYHVAPVVSVPELPSKFLVLDPSTFPVRPVPVETWLQKQNDPT
                     AVQNDSSHLAYELWLNGHQVFVDSTNPHLTNHKTGWESERMKFFFPLAPG"
     gene            11695..11832
                     /locus_tag="VGB76_12110"
     CDS             11695..11832
                     /locus_tag="VGB76_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099685.1"
                     /translation="MWRTTDQLHLPTEPRSTGKLHAHGHNAPPAYAPAHGLPRATRAA
                     R"
     gene            12073..13017
                     /locus_tag="VGB76_12115"
     CDS             12073..13017
                     /locus_tag="VGB76_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY0099686.1"
                     /translation="MVPKRVGLPYPPATESLLAIHSTDNPEAAIGEMLLRHEELRHRL
                     ELLEAEVTRLATETQCGTTLDTQHVERYDGTLGVSREFVRNFASPIGQLQWSSDLKHR
                     FRGAGDSPGDVSGVRWGSGSLIGPDLFITAGHCFDRAGGGWVRPKRKGVTISEEEIAT
                     LMRVNFNYQLNAETGDLRREDSYPVLRLLEFRRGSLDYAIVQLGRNERGEVPGEKYGA
                     LIVVASDLKTEGAMLCVIQHPNGEPKKVEAGTLLRNSNGHVYYDNIDTFGGSSGAAIL
                     SGETGEVIGIHTNGGCSAFSGANYGVSIGAIRHISSIL"
     gene            13402..13935
                     /locus_tag="VGB76_12120"
     CDS             13402..13935
                     /locus_tag="VGB76_12120"
                     /inference="COORDINATES: protein motif:HMM:NF042672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6624 domain-containing protein"
                     /protein_id="HEY0099687.1"
                     /translation="MNEALRAELKRMAREDQETREELLRAGKLPQDGYDSRMRRVHER
                     NNSRLREIVAQHGWPGRSVVGEDGCEAAWLVVQHAVLEPEFQRECLGLLERAVASGEA
                     QGWQLAYLTDRVLMYEGKAQIYGTQYVPVEGGKSAPYTIAEPEHVDARRRAVGLCSLA
                     ENTRRIQAEDELLNANL"
     gene            complement(14040..>14265)
                     /locus_tag="VGB76_12125"
     CDS             complement(14040..>14265)
                     /locus_tag="VGB76_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0099688.1"
                     /translation="WNLVKKWGEFTGVGKLSPHDLRRTAITRALDQGLSYRQVQMMSG
                     HKDPKTVMRYDHGRENLELNAANFLSYEEE"
BASE COUNT         2873 a         4289 c         4414 g         2689 t
ORIGIN      
        1 ggcgatgaag agttcgtcgg ttaaagggtt gaaggccaga ccttcgactt tggccgcgcc
       61 gccgcccgga gccgggacga tgccgcgaat ggcgagcgtg accgcatcga agatgacgac
      121 ggtgcccgtc gaggtgccgc agaaaagaaa gcccttgccg gaagccagcg cgcgacacgt
      181 cccgccgccg ggagggataa aggagcggac gacgttaaac ggagcgaccg ccttgatgac
      241 gtgaatgcgc gtgacggtcg agtagtagaa gaacgtcccg tcgtatgcca gcccttcgcc
      301 gcgtcccggc ggagtaggaa tcgtcgccgt ctgcgctccg gtgttcgggt tgatcgtgtt
      361 gattgaaccc gcgccgacgg tcacgttggc gacgcgaatg ttcgagccgt cccactccag
      421 actgccgatg gtgtcgttcg ccaccggatc gactgatttg agcaccgcgc ccgtcaacgg
      481 gtttagaaaa aatatcttct ccgtggacgg ctgctgtgtg aggaacagga gcgcgggtcg
      541 gaaggcgggc gagatgaaaa cgaagcgggc gaaggccaga cccgaacttg agccgctgtt
      601 cgtttgcgga aaagggaact tccgcaggac ttgaaatcct accggcggta acggcgtggc
      661 gttgagagcc ttgaaggcgt tgatgcgtcc ctttccgagc ttgcccgcaa aacccggatt
      721 cagcgcgtcg atgttgtcgc acgtgctttc gataatgttg cgcacctgct ggttggtcaa
      781 agtacgattc ttcgaccaga tcaagcccgc cagccccgcg acgtgcggcg aggccatcga
      841 agtgccgtta ttgaaaccga aggtgtcgtt gggaacggac gaaaagatgt tgactccggg
      901 cgcagacacg tcagtcgccg cgccgaaatt cgagaaggta gcccttttgt ccttctggtc
      961 ggtggcgaca acggcgataa cttcgggata gatcgccgga tactgcggag tgacgttgat
     1021 gtctgtgttg gcgttcccgg cggcgaagac caccaccacg tttttgttct tcgcgttgat
     1081 gatggcattg tgcacgcccg cgtggtcgcc gttcatcttc caactgcaat tgatgacgta
     1141 gcggcgcgcc gggttagcga cggcctgagc cgccacgtaa ttgatcgcgt cggcgcggtt
     1201 ctggttcatc ccgctcgtca ggttgacgcg caggggcatg atccggcatt tcggcgcgac
     1261 gccgacgacg cccgtagcat tgtcaacggc tgccgtcgtg cccgcgacgt gtgtgccgtg
     1321 gccgtccacg tcatcgggaa cctgatcggg accatccgcg aagtcccagt cttccgcgcc
     1381 tctgggcaaa aagttgggga gcaggtcggg atggttcata tccgcgcccg tgtctatgac
     1441 ggccatgatc acgtcggggt ggcctctggt aatgtcccac gcttccgtcg catctatgtc
     1501 ggcatccgcg ctgcccgcga tgccgttcac gacctgcccc gtgttgtgca gaccccagag
     1561 cttcgggaag tccggatcgt cggggatgta agagagcgcg tcgttgaatc cggcttcgct
     1621 cggctcggca aaagcgactt ccggcagggc ggagaattcc cgcagcgttt cgaagagtcc
     1681 ctttccttcg ggcaccgcca gcgtgtagta gccgggcgtt ctttgcacga cgacgacgcg
     1741 gcagccatgt tgttggatga tcttctcagc ctgagccttg ctgatctcgt cgcgaaactg
     1801 cactgtgaat tcgtcgggca gaaagtagcg cgttttgcct tcgtcatcga tcagcgcggg
     1861 aatcgcattg gcgatctggg gttgatcgct gagagtctcg gcggcgcggt ctgcatcctg
     1921 atcgggcgcg gcctgaaaca ctgcaaaccc acgctcgtag ttgattccct gactgatgga
     1981 gagggtcgaa gcgttcatgg cgtcgcgcgc catagattcg gtcggcggcg tttgaaaagt
     2041 tgccacgact tcatccgctt tgggggaaaa ctgaatctgt tttttgttga acttgttggt
     2101 gtaattgtac tcgttttcct tcttcacttt gggcttttcc tgtgtgctca tagagttcct
     2161 ttctctaacg acttgtcgat ccggcgattc acgcgagggg tgcagaccaa ctctctcaag
     2221 agtttcaact ctcctgctcc gcaagactcg cgtgccgcag gccgttattt gctcgcggat
     2281 atctccggca gagacatcat cttcgtgcaa atagttaagc caacatttga ttggcctgat
     2341 ctacccttct gatcggtgct gtgagacagt ctcactgtct cgtcgaggta agaaacgcgg
     2401 cgagttaaac gggttgaaga atatttacca tgacaggttt gagcgggtgg ctttcatctg
     2461 cgagcagtct tgatgtaggc agaccgggtt gcggcgtcat aattcacctt tgagcggcgg
     2521 cgtctcaacc gacggaatga ggagcctcgt cgcgcagcgt agcgcgctcc ggcaagagac
     2581 tgagatatga ctcgctcgct cgaattaaac tctgcatcac agagcagcaa tgaccgtgcc
     2641 actttcgcga gctgagcgtt tccgtcgata acggcgcgca gggtgcgcgg attgtctata
     2701 taaacagaca gggatgtcta caatagtaga ccaagttgac gggcgtagga gttcacgcca
     2761 caataagtct taacaggtat cttcgggtga gccgtatcgc tggaaagaca atgaaaccga
     2821 aaatcgtagc tatcgccgga gcggcgaagg gagccatctt cgagttggga gacgacgacc
     2881 tcgtcatcgg acgcgaaggg actaatcacg tctttctcaa cgacaagaca gtctcgcgcc
     2941 agcactgcat catcaaaaag gagaaggcgt tatataagat caacgatctg gagagccaca
     3001 acggaactct catcaacggc gtccccgtca aagagcagag cctcaggcac ggcgatcaca
     3061 tacaggtcgg cgacaacttc ttcctcttcc tgctccacga agagagcggc gcacccgtgg
     3121 caaacgatgt gagcttcgac gacgggacgg tcttcaacac gtcttccgtt cagttcagtc
     3181 tcgaagacgc gctctactcg atggcgcgcg acctgaatct cctgatcaaa ctcggcgaga
     3241 tcgtcaatac aatccagtct ttgaaggcgt tgcaacgccg cctgctcgaa tccatcttcg
     3301 aggcgattcc ggcagagcgc ggggcgatac ttttgataga cgagagcctg gaaaatcccg
     3361 cgtcggtctt cgccatggac agagttcggg gtccggcgca tccggttcat gtgagccgga
     3421 cggtggcgcg cctggtcttg agcgaggggc gggcggtgct cagtaacaat gtcgtggaga
     3481 gcgacacgct ggcaaaatcc gaaagcctga cggcggcgaa gattcattcg ctgttgtgcg
     3541 tgccgctgac gctgcacaaa cgtatcaccg gcatcatcta tctggagacg agcgagtcgg
     3601 gcgcggtgct caacgagagc cacctgcgtg tgctgagcgc aatcgccagc ttcgtctcgg
     3661 gcgtgctcga caacatgcga catgtggagt ggcttgagag cgagaaccgg cgtctacagg
     3721 gtgacatcca cgccgagcgc aacatcgtgg gcgaaagcgc gcggatgcag gagatctttc
     3781 agttcatcgc caaagttgcg cccacggatt caaccgtcct gctgcgcggc gagagcggca
     3841 ccggtaaaga gttggtcgca cgcgcgattc accgaacgag cacgcgcgcg gataaaccgt
     3901 tcgtcgccgt caactgcgcc gcgttgaccg agacgctgct cgaaagtgaa ctgttcggac
     3961 acgagaaggg gtctttcacg ggcgcgtacg cgcagaagaa aggaaagttt gaaatcgccg
     4021 aaggcgggac tatattttta gatgaagttg gcgagatgtc gccgccagcc cagacaaaac
     4081 tgctgcgcgt gctacaggag cgagagtttg aacgggtggg cggaacgcgc ctgattaaag
     4141 cgaacgtgcg cgtggtcgcc gcgacgaaca agagcctcga aaaagccatc gagaacggcg
     4201 cgtttcgcca agacctctat tatcgtctga acgttatctc cagagtgatg cctccgctgc
     4261 gtgagcgggg cgaggacatt ccgctgctgg cgagctactt cgtcatcaag cacagcgcga
     4321 agtgcaagcg gcgtgtgacg ggactctcga cgcaggcgcg cgccatcctc gtcggctatc
     4381 attggccggg caacgtgcgc gagttggaga acgccatcga gcaggcggtc gttttaggat
     4441 cgatggacag ggttcagccg gaagacctgc cggaacacat tctcgaagct tcgggcgcgc
     4501 cgggcgaggg cggaatgaac tatcacgagg ccgtccgcga ggcgaaaaaa cagcttctcc
     4561 aaaaggctct caatcaagcg ggcggcagct acaccgaagc ggccaaactg ctcggcatcc
     4621 atcccaacaa cctgcaccgg ctcgtgcgca acgtcggctc caaaacgacg ccggaaaagt
     4681 gacgctgaca cgcccggagc aggcgcgccg gacggcggcg acgagcgaga ctgtcgccgt
     4741 ctcagggtat caaatcttcg agcacccaga tgttcccgtt cgtctcggtg atgggcagga
     4801 cgagaaagtc cctgttgagc gaaatctcca gataggagag cgaggcggcg accatccgcg
     4861 cggggctgcc gaggtttgtg acgcggaacg gctcgcccac ggttctgccc tgttcggggt
     4921 cgaacctgat gccgtagacg ttcaggaaat atgagttctg gttgtagatg aagtagagcg
     4981 tgcggccatc gggcgaccag cgcggcttat cagcccaagc cttctcgtcc gtcacgcgca
     5041 caggcgcgcc gcccgacgcc ggggcgacat agaggacgga cgaggccgtc tcgttcgggt
     5101 cttgcttgag aaacgtaatc cagcgaccgt ccggcgagaa gcgcggagtc cagagattga
     5161 cgttcgcctc ttccagcact ttgcgcagct tcaactcggc gttcggcgca ccgtccaacg
     5221 gcagcatggc gatgtaccaa cgctccggcg agccacggtc ggaactcacc agcacccact
     5281 ggccgtcggg cgaccaatcg taattgtaat cgcgccacgg cgacgcctcc cctgcgggcg
     5341 ggtgtgaagc catcgtctgc tccgcgccgc tcgcgctgtc gagtatgacg accgagccgc
     5401 cgcgcttgat cctgcgcggc gggatcaact cgtggcgact gtaagcgatg cgcgttgagt
     5461 ccggcgacca gcgagggtta aagggaaccg cgccttcaac ctcggaaaga agtcgctgct
     5521 cggagttgac cagagacttc tcccagattt cgtgcttggt ctgcccctgc cggatgaccg
     5581 agtagaccag tttcctgccg tcgggcgaaa tgtccgggaa ccatgcctcg acatccttcg
     5641 gggtaaccgg atgaccttcg cccttcacca gcccacggcg cgcgtcgaag ggcagcaacc
     5701 agaggctcgt gccctgactc ttcttcgtgt aggccagctt ccgcccgtcg cgcgcgaggg
     5761 cgatctccgt gtcctgtccg tcgctcgtgg tcaggcgttc cggtccaccc gtccactgca
     5821 tgctcttcgg atcaacctgg atccgccaca ggttcagaac gccgttggag cgtccttcga
     5881 ggtagagcga cgcgcctgac ggcgaccact ggaacgcgcc ccaatcaatc gcagccttac
     5941 gcatccgcgc ggccacctct tccgagactt ccgatttaac gggcgttccg ccttcgatgg
     6001 ggatcgtgta gaaggctctg tcctcgcccc agtaagaaac cctccggctg tcggggtgcc
     6061 aggccagtgc gccgcgcctg acgccgcgct cgtcgcccat cggcgaggtg agcacctcgt
     6121 gcggcgcaga ggcgccgtcc ttgtccagca cgtagacgcg cgggcggtcg ttcagacgca
     6181 ttccctgtcg caggaagaga atccgcgagc cgtccggcga ccagcgcggg ttatagccga
     6241 acgaagagat tttgcgcacg cgcttgccga agggcgcgct tgtgaagtag aggccgccgc
     6301 cgtcgcgctc ggagcgaaag acgatcaatg tgccgtcggg cgaccaatta ggttgccagt
     6361 cgtgctccgg cgagtctgtg atgcggagcg ggtcgccgct gctggcggat ttcacccaga
     6421 tgtcgaagtt gccgtcgcgg tctgaactgt aagcgatgaa ctggccgtcc ggcgaccatg
     6481 tcggactgcc ctgcaagccc tgctcgaacg tcaggcggtt caggatgaga cgctgcggcg
     6541 gagcggcggg cgcggcggtg cgcgcggcgg tcagcaggta gacgcccaac gtcgccgctg
     6601 ctacgaagca caggatcgcc gcgacggaga cttttcgatg tcggtgcagc gtggagacga
     6661 tgtactccgc gctcgacatc gagtgcgtcg atgaaggcga cgctggcggg gcgtccttcg
     6721 gggagctttc cgtcgaggat gctctctgcg gcggccacga accgccgggc acgcttgcgc
     6781 cgccgctgtt caacgcttcg agcgaagtgc gttcgccttc cgcccctgtt tcaagacgac
     6841 gcttgagacg gcgaaggtcg atgtgcatct cgcgcgccgt ctcataacgc tccgcgcggt
     6901 ctttcgccaa cgccttcaac gccacccatt cgacggcctc cgtcacttct ctggagaagc
     6961 gcgccagcgg cagcggcggc tggtgaagaa tcgccgcgat gatgtcgctg ttggacgctc
     7021 cctcaaacgg agtccgcccg gcgatcatct cgtggaacac gacgcccaga ctccaaatgt
     7081 ccgtgcgcgc gtccatcgtc aaaccgcgcg cctgctccgg cgacatgtag gcgacggtgc
     7141 ccatcatcac gcccggctcg gagaacgcac gcgaaaccgt cgaattattc ggttcctctt
     7201 cgtgcggagt cgcctcggac accttcgcca gcccgaagtc caagacctta acgtgaccct
     7261 cgcccgtcac catgatgttc tccggcttga tgtcgcggtg aacgatgccg cgttcgtgcg
     7321 ccgccgcgag cgcgccgcac acctgtagcg caacgtcgag cgcatcgctc aaactcatgc
     7381 gtgactttcg caggcgcgcg cgcagcgtct cgccctgcac atactccaac gcgatgaagt
     7441 tgacgccctc cgcttcctcg acttcgtaga tgtaagcgac gttcggatgg ctcagggctg
     7501 acgccgcacg cgcctccttg aagaagcgct gcacggcgcg cgggtctgcc tgcgcgaccg
     7561 cgtgcagaac cttgagcgcg acggagcgtt cgagcttgag gtcgcgcgcc aggtagacct
     7621 cgcccattcc gccggagccg agcagagatt gaatgacgta gcgtccgatc cgggtgccgt
     7681 ttgcgaaggg cattgtgcgc gggtctgcga agagaaatag cggtctgggg cggatgtaac
     7741 tgtattacac ggcgaaggat tgtcaagcgg gtatcttgtc gcgtcgagaa aaaatgcgcg
     7801 gcacgggcgc gagcggcggc agcgattgac gctccgcacc tgacacgcgc cggaacgtcg
     7861 ccaaatttaa ttattttcgt gtgaacgaga cggcatcgcc cccgcgtgcg agccgcaaaa
     7921 ggcgttgctc agaaggtgaa cctgccgtaa aactccccgg cctcgcgcag ttgcgccagt
     7981 atctctccga gaaccgttcc gcccggcgca tccacctgcg gcttccagac ggcgcgcgcc
     8041 tgctcgtcgt agtggaagtg atgtttactc catctctcgt cgccgtagca gtagtgaatc
     8101 atgtcccaca cgatgggcgc gtccatgcga tgactgaagc cgagcatgta agtgagttcg
     8161 atccgcatct tcaacttgac ggcggccaac ccgagtgcgt acatgctgat ctcccagagc
     8221 accgggggaa aggcgtcaat cgcctccagc catgtctccg ccaaacgctg cgcgtcagcc
     8281 gacgggacaa catgcggcac gccgaggcac agttcactcc cgcgactttc gatctcgcag
     8341 gccgcaacgc ccaacttctg tcgcgccgtc tggacggctt cctgatcgta tcgcatgtaa
     8401 gggtagtatt cgcccgacaa catgcgcggg aacttcggtt cgttgacgaa gagcatgtcg
     8461 gggtcgcaga gcacgatgag atcgtcagtg ccccggtaca gttctgcggc gtggaggagc
     8521 gtgccgggag tgttgcgcgg cgaatagcgt tgcccttgcg gcgtcagggc gtagctgggc
     8581 gcgtggcgca cgatgcctcc ggcgcgcacg atgtcgcgga aatccgggtg ccagtcttta
     8641 cccgattcgt ggacgatgaa ggtcggcgtg aggcgcaggc gactgacgca ggagaagtgg
     8701 aacagcttgc actgccacgc cataaaagga ttgttctcgg ctgagacgac gacgcggtac
     8761 tcgctcatct atcgaacctc cggcctgcct taaatccaac gcacgggcgg cgcaccgcac
     8821 aaccatgtca cgacggcata cttctccccg gctgtgaccg gctcggcgca gtgcaggtat
     8881 gtggcgggga agatgatggc tgcgccggtg cgcggcttaa aggaataatt gagggcgggg
     8941 aaagtggtct gcccgccctc gaactcttcg tttagatagc agagcacggt gaagtacctg
     9001 taatcgtcgc cggggatcgt atcggtgtgt gcgacgtaaa aatctccggg cgcatagcgc
     9061 acgatatgcg tcgccttgtg cgtctccaga ttcgcctgcc agacacggtt gacgagaggc
     9121 cgcacgatgc gtgccatctg ctcgtcaaac tcccgcccga ccggcgagcg actatcaaaa
     9181 gcgagggcgc gcgccgaacg gtaagcggga cgcgccgccg catcgcctgc gccgcgcccc
     9241 ctttcgaaaa tggacgccgc cgtccacccg tccgccgcgc gcgcatactc gacgaggccg
     9301 cgacagtagc gagggctata aagctgtgtc gagatgatgc ccgctacttc ttccgtgtat
     9361 tccagaggcg cagcctcaag cacagccttc ttcacgccaa cctccacaga ctatttccgc
     9421 cgccccgaac ccgcgcgaca aactcagacc agcgccagcc tacgccggac acttcacggc
     9481 gacgcgcact gcccgaaggt gcagcggctc aactgctgcg ctccggcggt tgcggaccaa
     9541 tccctccgcc ggggggctgc ggcccaatcc ctccacccgg gggttgaggc cctatgccgc
     9601 cccccggagg ttggggaccg atgccgccat ccggaggctg agggcctatg ccgccatcgg
     9661 gcggctgtgg ccctatgcca ccatcaggca cctgcggtcc caaggacggg gtctgcgttc
     9721 cctgcgactg tggcctgtac gcctcgccgg ggtcgatgcg ggagccaccc atcaactcgt
     9781 cgggcactgc gagcatgagg ttcctcagca ggtcgtcttc caccgagacg ccgtgctcgc
     9841 gcaggagctt tgacagcagg gcttggaact ccaggttaag gcggttgttg atctgaaagg
     9901 tttgccgcaa gaccagcatc ctgcgaacca tttcttgccc gttcataatt ttgttgcctc
     9961 ctcaaagact gttaacgccc gcgcacaacg ggcagagact aacccggcgc aagcgggaag
    10021 aagaatttca tccgctcgct ctcccagcct gtcttgtgat tagtaaggtg cggattggtt
    10081 gaatcaacaa acacctgatg accgttcaac cagagttcat aggccagatg cgacgagtcg
    10141 ttttgaactg cggtagggtc gttctgcttc tggagccacg tctcaaccgg gactggcctg
    10201 accggaaacg tcgaggggtc gagcactaaa aatttagaag ggagttccgg caccgaaacg
    10261 acgggagcca cgtgatacct ccacagcacg tagccctccg ggtcgttgtc ggtgacgaac
    10321 ctgagtgtcg ccctgttgtt cggatagttg ttgacaagcc cggttccgta attccaaatc
    10381 ttcctcgcgg tgatgccgcg ctgttccaac aagcggcgac acatcaggtg agcgcgagcc
    10441 atgcaacagt cggtcgggta cctgaaagga atttgcaact ccgggatcgc ggcgacgaag
    10501 tcgaaggctt cgagagcctg cgagatcgtc agatgcgaaa tgaaagaagc cggcaacaac
    10561 tccctgaacg caaacggcag cacgtccttg agcagcggca tttcgagcac gtccaccggc
    10621 ccgttcgcca agcccggcaa gttcagatcg acgggcgcag ggcgcacgtc aacgatctcg
    10681 cgcttgaaag tcgtgcaggt caccagcacc ctgctgtggt cttcccatgc gtccttgaga
    10741 atctttctga gttcttcaaa tttcgggtgc gagccgcgca gcggagagac ggctggcgac
    10801 tgaaagaaac tgacctccag cccgctgccg tccgccgccg tttcaataaa ctcgacggta
    10861 tagagataag tggggatgac ctcgtagact tctcccgtct ccgtgtcggc ctcgacgtag
    10921 acggggattc ccgacttgtg ccgcgattcc agatactcca cccagattcc gaaacgcggg
    10981 ttcgttctga ttaacctgaa gaccgactcg tcgccctcat ccccgatgac gatctcgatg
    11041 tcgtcgtcta aggcggcgcg gcctgcctcc ttcgcaactt ccaacagggg ggcgccgtcg
    11101 ccgcgccttc tcatctcccg taccttcccg accttcgctt gcaatgcgcc catcacataa
    11161 atccttcccc ggctgaaaaa ctttacctgc gcgactatcc cttcaaacga tctctcgctt
    11221 gatccggcaa acgccgaaag cggctcgcgc tcgttgcctg tgccggataa gtgccagccg
    11281 gtgcaatcac agtgccatgc taccggagcg gacacacacg tgcgacacac gactcgacac
    11341 tgctccggcg cgtagtctcg ccgaagtcgc agagactatc aaacaaaagc catacttgta
    11401 gaattgaggc tgaacagcag cacgggcaga agcttcgaca gagaaggtgc tgaggtcagc
    11461 cgacccgaaa aaacccggcg aaaacttgtg cgagcaagcg taaatcggcg cgtcgaatct
    11521 gtcaacgaga ctgccaaaga aagttctgca cgggtcttgc tgcccaagcc gacacgtcgg
    11581 cgagcgtcgg cttgcggtcg cgcagcatgc ccgcgcgcca acgtgaagca ggcacgcgcg
    11641 gcctgtagcc cggggcgcgg cctatcaata ttgattgtcc gaatatcaaa attgatgtgg
    11701 agaacgacag accaacttca tcttccaacc gaaccgcgca gcaccggaaa gctgcacgcg
    11761 cacggccaca acgcgccgcc ggcgtatgcg cctgcgcatg gcctcccgcg cgccacgcgt
    11821 gcggcacgct aatcgccaca gtccttctcg tgtgtagccg cgtttgatcc tttgcgacga
    11881 ttcatagcgc gttcattcgc ccaccggcag gacgaataca catgccgctg cacccgctta
    11941 tttaaaaaca tttgagctta ataacatgaa gacaggatgc gggcggggct acacgccggg
    12001 cgcgctctgc gcaagagttc ttcaccagct gaaacttgtc gtcccgcaac gcacgcggaa
    12061 aggatgagat aaatggtccc taaacgagtc ggcctaccat atcctccggc gacagagtcg
    12121 ctgctggcta ttcacagcac cgacaacccg gaagcagcta tcggcgagat gctcttgagg
    12181 cacgaagaac tgaggcatcg actcgaactg ctcgaagcgg aggtcacacg gctggcgacc
    12241 gagacgcaat gcgggacgac gctcgacacg caacacgtag agcgttacga cggcacgctc
    12301 ggcgtgagca gggagtttgt gcgcaacttc gcctcgccca tcgggcagct tcagtggtcg
    12361 agcgatttga agcacaggtt caggggggcg ggcgactcgc cgggcgacgt gagcggcgtg
    12421 cgctggggat cgggttcact cattggtccc gacctcttca tcaccgcagg gcactgcttc
    12481 gaccgcgcgg gcggcggctg ggtgcggcct aagcgcaaag gcgtaaccat ctccgaagag
    12541 gagatcgcca ccctcatgcg cgtcaatttc aactatcaac tcaacgcaga gacgggcgac
    12601 ctgcgccgcg aggacagcta ccccgtgctg cgcctgcttg agttccggcg cggcagtctc
    12661 gattacgcca tcgtgcagtt gggcaggaac gagagggggg aagtgcccgg cgaaaagtac
    12721 ggcgcgctga tcgtcgtcgc gagcgatctc aagacagagg gcgcgatgct ctgcgtcatc
    12781 caacacccga acggcgagcc gaagaaggtc gaggccggga cgctgctccg caacagcaat
    12841 ggccacgtgt attacgacaa catagacacg ttcggcggct cctccggcgc ggccatcctc
    12901 agcggcgaga cgggcgaggt catcggcatc cacacaaacg gcgggtgcag cgcattcagc
    12961 ggcgcgaact acggcgtctc catcggcgcg atacgccaca tctcaagcat cctctaggca
    13021 cgacgaatca cagccacgca cctccggagt ctgataacct atatccaaca agtaagggcc
    13081 agactgctaa cggcgaataa cagcgaaggc tttcgagcct attctcttgc cgccttgatc
    13141 ggggagagat agattgttgc gctaccggga gagctttttg tcaggggttt taagtcacgt
    13201 gcgccaattg acgacatacg tataagcaag aaagccgggg ttgcaaatct acagcagaga
    13261 aaccgttgac gcatcgaggc ggcatccgag ttcgacgcgc ggcggagggt tggcgactcg
    13321 cgttggttcc acgttcggcg tgccggaaat gaccggacga ggctcaaaca ttcgaagcaa
    13381 gaagccggag tgtgtgtgta gatgaacgaa gcgttgcggg ctgaactcaa acgcatggcg
    13441 cgcgaggatc aggagacgcg cgaagagttg ttgcgcgcgg ggaaactgcc gcaggacggt
    13501 tacgactcgc ggatgcggcg cgtccacgag cggaacaact cgcgtctgcg cgagatcgtg
    13561 gcgcagcatg gctggccggg gcgcagtgtg gtgggcgagg acggttgcga agcggcgtgg
    13621 ctcgtcgtgc agcatgccgt gcttgagccg gagtttcaac gcgagtgtct gggcttgttg
    13681 gagcgcgcgg tcgcgtcggg cgaagcgcag ggctggcaac tcgcgtatct gacggacagg
    13741 gtcttgatgt acgaaggaaa ggcgcaaatc tacgggacgc agtatgtgcc ggtcgagggc
    13801 ggtaagtcag ccccttacac catcgccgag ccggagcacg tagacgcacg caggcgcgcg
    13861 gtcggcctct gttcgctcgc agaaaacacg cggcggattc aggcggaaga tgaactcttg
    13921 aacgcgaatc tctaaattgc gtcgcggatc acagcagttc aggtggacga ggcgcggctc
    13981 atttaaaccc gacaggaagc gcgcgcctga gtggctatgc ctcaagttgc gtctcacttt
    14041 tactcttctt cataagagag gaagttggcg gcattgagtt ccagattttc ccgcccgtgg
    14101 tcgtaccgca tcacggtctt agggtctttg tgcccgctca tcatctgcac ctgacgatat
    14161 gaaagcccct gatcgagcgc gcgcgtgata gccgtccgcc tcagatcatg cggcgagagc
    14221 ttacccacgc ccgtaaactc tccccacttc tttacgagat tccag
//
LOCUS       DASWNS010000347         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_453416, whole genome shotgun sequence.
ACCESSION   DASWNS010000347 DASWNS010000000
VERSION     DASWNS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453416"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VGB76_12130"
     CDS             <1..1325
                     /locus_tag="VGB76_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099689.1"
                     /translation="PGLLVAFVFELTLDYFSTAPYTTRYAIVVFNRLALFVSIVLFAS
                     SRREAEKRLRDQREWLRVSLESIGDAVIATDIDGRVNFINPTAEALTGWKLSEAVNKP
                     LGEVFRIISEDARQPVENVFTVIRREGIVVGLANHTILIAKDGKEIPIEDSGAPIKDK
                     SGKMIGVIVVFHDVSERRRAEQERIESLKREQAARSEAEAANRLKDEFLATVSHELRT
                     PLTAIVGWASMLRTINFTPESTARALETIERNAKAQTQIVEDLLDVSRIITGKLQINS
                     RPVAIAPTVEAAIATLRPAAEAKAMTLKLSLGEATGLVFGDPDRLQQIIWNLVSNAIK
                     FTPENGRIEVRVEQSGAFFELSVRDNGIGIEAEFLPYVFERFRQADSSTTRTHGGLGL
                     GLAIVRHLVELQGGTVSAQSDGAGRGSVFTVRLPLASSREEVVMSEVK"
     gene            complement(1600..>3087)
                     /locus_tag="VGB76_12135"
     CDS             complement(1600..>3087)
                     /locus_tag="VGB76_12135"
                     /inference="COORDINATES: protein motif:HMM:NF005557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxyadenosine deaminase"
                     /protein_id="HEY0099690.1"
                     /translation="PESNHTNSRRRSVFAQTKDAPAAAELLLLYSTFAPKHNRREVYL
                     SLSGTFKDRRMSNSILIKGGAIITMNAGDEIVTGDLLIRDGRIVSIGETTEAADEVLD
                     ARNCAVLPGFVQTHIHLCQTLFRGAADDLPLIDWLKRRVWPLEAAHTRASVRASARLG
                     IAEMVKGGTTCALTMETVHHTEEVFRVVEETGFRATVGKCMMDAGAEVPAGLFEQTAD
                     SIRESLSLIEAWHGRASGRIRACFAPRFAVSCTRELLEGVARLSRERGVLVHTHASEN
                     RTEIEMVESATGMRNIAYLASLGLAAPHVALAHCIHLDESELAILAESGTHVMHCPSS
                     NLKLGSGIAPIVEMLARGVSVSLGADGAPCNNRLDMFTEMRTAALLQKVAHGADALPA
                     RRVLRMATVEGARALGIAHETGSLETGKRADVILVALDRLHLTPRPDIVSAIVYAAEA
                     SDVRTVIIDGRVVMRERELLCVNEAEVLAEAGAQAELLFARANIG"
BASE COUNT          604 a          990 c          916 g          577 t
ORIGIN      
        1 gtcccggcct gctcgtcgcc ttcgtcttcg aactgaccct cgattatttc tccaccgctc
       61 cttacacgac gagatacgct atcgtcgtgt tcaaccgtct ggcgctcttc gtgagcatcg
      121 tgctgttcgc cagttcgcgc cgcgaggcgg agaagcgtct gcgcgaccag cgcgaatggc
      181 tgcgcgtatc gctcgaaagc atcggcgatg ccgtgattgc gaccgacatc gacggtcgcg
      241 tcaatttcat caatcccacc gccgaagccc tcacgggctg gaagctctcc gaagccgtca
      301 acaaaccgct cggcgaagtg tttcgaatta tcagcgaaga cgcgcggcag cctgtggaga
      361 acgtcttcac ggtcatccgg cgtgagggca tcgtcgtcgg gcttgccaac cacaccatcc
      421 tgatcgccaa agacggcaaa gagattccga ttgaagatag cggcgcgccc atcaaggaca
      481 aaagcggcaa gatgatcggc gtgatcgtcg tcttccacga cgtgagcgaa cgcaggcgtg
      541 ccgaacaaga gcgcatcgag tcgctcaaac gcgagcaggc ggcgcgcagc gaagcggaag
      601 ccgccaatcg cctgaaagac gaatttctcg ccaccgtctc gcacgaacta cgcacgccgc
      661 tgacagccat cgtcggttgg gcgagcatgc tccggacgat caatttcacc cccgaatcca
      721 cggcgcgcgc ccttgagaca atcgagcgca acgcgaaggc gcagacgcag atcgtcgaag
      781 acctgctcga tgtctcgcgg atcatcacag ggaagttgca gatcaattcg cgcccggtcg
      841 ccatcgcccc gaccgtcgag gccgctatcg ccaccctgcg tccggcggcg gaggccaaag
      901 ccatgacgct caagctctcg ctcggcgagg cgacgggtct ggtgtttggc gatccagacc
      961 gcctgcaaca gatcatctgg aatctggttt cgaacgcgat caagttcacc cctgaaaatg
     1021 gccggatcga ggtgcgggtg gaacagtcgg gcgcgttctt cgaactcagc gtgcgcgata
     1081 acggcatcgg catcgaagcg gagtttctgc cttacgtctt tgaacggttc cggcaggctg
     1141 actcttcaac gacacgtacg cacggcggct tgggcttggg actggcgatt gtgaggcatc
     1201 tcgtcgagtt gcagggcggg acagtgtcgg ctcaaagcga cggtgcgggt cgcgggtctg
     1261 tcttcacagt gcgtctcccg ctggcctcta gccgcgagga agtagttatg agcgaggtta
     1321 aatgaagcaa attcgcgggg taaataattc ctgtcaactt tgtcggatta gtcacaaaaa
     1381 agcgttgaca gttttgtgat agcgtgtata atcccaccga gttttggatg agccggtcgc
     1441 cacaacctgg ttagtcgcaa gacagcatcc gagacagata cttagacgcc cgcccgtgcc
     1501 agctcgaacg ccgaagcgcc ccccgcttac gcaacattcg agctggcaca gctttttttg
     1561 cccgccgaac tttccaccag cgacatccgc agccgtctct cagccaatat ttgcccgcgc
     1621 aaacagaagt tccgcttgcg cgcccgcttc ggccaacact tccgcctcgt tgacgcacag
     1681 cagttccctc tcccgcatca caaccctgcc gtcaatgatg acggttcgca cgtccgacgc
     1741 ttccgccgcg tagacgatgg ccgagacgat gtcggggcgc ggcgtgagat gcaggcggtc
     1801 gagcgcgacg aggatgacat cggcgcgctt ccccgtctcg agcgaccccg tctcatgcgc
     1861 gatccccagc gcacgcgcgc cttcgacggt cgccattctc agcacacgcc gcgcgggcag
     1921 cgcatccgcg ccgtgcgcca ccttttgcag cagcgcggcg gttcgcatct ccgtgaacat
     1981 atcgaggcga ttgttacagg gcgcgccgtc cgcgccgagc gagacggaga cgccgcgtgc
     2041 gagcatttcg acgatgggag cgatgcccga accgagtttg agattcgaag acgggcaatg
     2101 catgacatgc gttccgctct cggcgaggat tgccagttcg ctctcgtcca gatgaatgca
     2161 atgcgcgagc gcgacgtgcg gcgcggccag accgagcgac gccaggtatg cgatatttcg
     2221 catccccgtc gcgctctcga ccatctcgat ctcggtgcgg ttctccgaag cgtgcgtgtg
     2281 cacgagcaca ccgcgttcgc gcgagaggcg cgccacccct tcgagcaact cgcgcgtgca
     2341 actcacggcg aagcgcgggg cgaagcacgc acgtatgcgt cccgaagcgc gcccgtgcca
     2401 cgcttcaatc aacgacagcg attcgcgtat cgagtcggct gtctgctcga aaagtcccgc
     2461 cggaacttct gcgcccgcgt ccatcatgca cttgccgacc gtggcgcgaa atcccgtctc
     2521 ttcaaccacg cggaagacct cttccgtgtg atgcaccgtc tccatcgtga gagcgcaggt
     2581 ggtgccacct ttgaccatct ccgcgatgcc gagccgcgcc gaagcgcgta cggaggcgcg
     2641 cgtgtgcgcg gcttccaacg gccacacgcg ccgcttcaac caatcgatca agggcaggtc
     2701 gtccgccgcg ccacgaaaga gcgtctggca gagatgtatg tgcgtctgca cgaagccggg
     2761 aagcactgcg cagttgcgcg cgtccagaac ttcgtcggcg gcttccgtcg tctcaccgat
     2821 tgacacgatc cgtccatcgc gaatcaacag atcgcccgtc acgatctcgt cgcccgcgtt
     2881 catcgtgatg atcgcgccgc ccttgatgag aatggagttg ctcatgcgtc tatctttaaa
     2941 cgtgccggac aacgacaggt aaacttcgcg ccgattatgc tttggcgcga acgtgctgta
     3001 aagaagcaaa agctcagccg ccgccggagc gtctttcgtt tgtgcgaaga cgctccggcg
     3061 gcggctgttg gtgtggtttg actcagg
//
LOCUS       DASWNS010000348        13643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_247425, whole genome shotgun sequence.
ACCESSION   DASWNS010000348 DASWNS010000000
VERSION     DASWNS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13643
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247425"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1434)
                     /locus_tag="VGB76_12140"
     CDS             complement(100..1434)
                     /locus_tag="VGB76_12140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099691.1"
                     /translation="MKKRRNLLLVLFFTALTVLLTGVVVLLWEKVLMAPFYSVIEAYY
                     PGAANSVARWRTQQRFEHFFISVTVDLIVVSLLLRLVSRQQRKLVASEERYRALFERA
                     NDGIVIVSAANRSVIEVNDKFCELFGCRPQELLGKNINELDWRTQGDVDGDTLAIALN
                     DLNCTDRELSIHTSKGLALPVSVSCSTLSMDEERLRVLIVRDWSIRKRLEAEKEEMQR
                     QLFHNEKISALGRVAAQVAHEVKNPLAGLLLYAMHLKNKLGSKLTESEVALCDRIINS
                     INHLVGTTEQILSFARPVSLSPRPVDLNLVVRKVCQLLQPQIDAHRIDERLELADAEV
                     PTLLDETSITSALINLTLNAIQSMPDGGRLTVKTNRVNGTSQLTIADTGHGMTPEQIE
                     KVFEPFYTTKSQGLGLGMPYAKKVIELHRGAIHIESRREAGTQIIIELPAEK"
     gene            complement(1743..2459)
                     /gene="aat"
                     /locus_tag="VGB76_12145"
     CDS             complement(1743..2459)
                     /gene="aat"
                     /locus_tag="VGB76_12145"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY0099692.1"
                     /translation="MSETDISYRHPAEFFKRDAELIRFPDPRDAPYGDIVAVGGNLHT
                     LNLLRAYERGIFPWPIEGWPLTWFSPLERAIIEFKDLKVPRSLAKVRRRAPFRLTIDA
                     AFRAVILACAGVARPDEQGTWITPQMLEAYCRLHELGHAHSVEAWEGDALVGGLYGVD
                     AGGAFAGESMFYLRPNASKLALLHLIENLQLRGLDWLDIQVMTPHMKALGARTIPREE
                     FLDKLAAARRRKLKLFDAMS"
     gene            complement(2564..4846)
                     /gene="clpA"
                     /locus_tag="VGB76_12150"
     CDS             complement(2564..4846)
                     /gene="clpA"
                     /locus_tag="VGB76_12150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY0099693.1"
                     /translation="MITRELQATLSAAVEEAIERRHEYVTLEHLLLALLSDSTASNVI
                     RHCGGDTDALRRDLELYFDERIELVTRNTTHIPEQTDTFTRTLQYAMLQAQGSGQKEI
                     DGGNILAALFQAERSYAVYLLKEQGITRLDVLNFIAHGISKLAGGYEPAGNEFDGATE
                     GEADAGQERDPLAAFTVNLNERAREGFIDPLIGRSVEVLRTIQILCRRRKNNPIYVGE
                     PGVGKTAIAEGLALKIQQGDVPEVLRDAVVYALDMGAVLAGTKYRGEFEQRFKAIINA
                     LKKIPAAILFIDEIHTIVGAGAVSGGSMDASNIIKPALASGELRCIGSTTYAEYKSAF
                     ERDRALARRFQKIEIGEPSLAETVEILHGLKSYYEKHHGVTYAPEAIQLAAELAAKYL
                     HDRLLPDKAIDVMDEAGAAMKLLPKEERPETVGTAEIEIVLARMAKIPPKTVAGSERD
                     RLRDLKGELQAVIYGQDQAIEGITNAIKLSRSGLGNPEKPVGSFLFSGPTGVGKTELA
                     KQLAHALGIEFIRFDMSEYAEAHTVSRLIGAPPGYVGFDQGGLLTDAINKTPYAVLVL
                     DEIEKAHPNLFNILLQVMDHATLTDNNGKKADFRNVILIMTTNAGAQELSAGGIGFQS
                     NTSGKGQGRGAIERTFSPEFRNRLDAWIAFSPLSFEVIEKVVDKFIKELNAQLAERNV
                     RVVLDEAARSWLASRGFDRLFGARPMGRLIQSKIKEPLAEELLFGTSLASGGEVSVRV
                     ANEELTLVFPGLEVNEAKGN"
     gene            complement(4929..5249)
                     /gene="clpS"
                     /locus_tag="VGB76_12155"
     CDS             complement(4929..5249)
                     /gene="clpS"
                     /locus_tag="VGB76_12155"
                     /inference="COORDINATES: protein motif:HMM:NF000672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY0099694.1"
                     /translation="MSRFDSDTDEAVVTESETRLKKPPLYQVVLHNDDFTTMEFVVYV
                     LQNVFQRSEVDALRIMLQVHLEGVGVAGVYTYEIAEMKVSKVTELARANEFPLLCTIE
                     ETSV"
     gene            complement(5511..5660)
                     /locus_tag="VGB76_12160"
     CDS             complement(5511..5660)
                     /locus_tag="VGB76_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099695.1"
                     /translation="MSTIVTLAALLLALTILNSNASKGINPVQHSERTASQASKGINP
                     VQHSE"
     gene            complement(5813..6484)
                     /locus_tag="VGB76_12165"
     CDS             complement(5813..6484)
                     /locus_tag="VGB76_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY0099696.1"
                     /translation="MSTQLEKIRSQKYIGVVELAKQAARILAEIGTTQDRGTVSEMPD
                     ERTLRYYLAEGLLSPASDKQGTASVFGYVHLLQLLVVKKLQSEHLPIRKIKELVDGRS
                     ERELERLLGLDAKAGAKNEAHSYLEKLLTRSTSPAAASTPSRKQNFASPADAPLAKPT
                     PNLATQRQAAPSSPATWERVEIEPGLELHVRGDYAPPSDAKEVRRLTRLLLSALEGQR
                     GSKGK"
     gene            6634..6885
                     /locus_tag="VGB76_12170"
     CDS             6634..6885
                     /locus_tag="VGB76_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099697.1"
                     /translation="MTKRGKYLRASEVAEYVYCARAWRLRVDGREPASGHAQRAAGEA
                     WHLAHGAAVVRARRMRLLAGFAFALALIVGLLILLLGWR"
     gene            6876..7451
                     /gene="cas4"
                     /locus_tag="VGB76_12175"
     CDS             6876..7451
                     /gene="cas4"
                     /locus_tag="VGB76_12175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEY0099698.1"
                     /translation="MALSLLYLAGIVLLLVALGAWLASRRGARQADLPEGRIIYSDTG
                     APVGRIAPVTLSERGERQEKPLLSHRHALVGRPDYLVQTEGGIIPVEAKSANCPASGV
                     PYESHLMQLACYCLLVEEATGATVPFGVIRYRDRQLRVDYTDELRESLLELLAEMREA
                     RRAGEVHRSHEEAARCAACSYREICVESLAD"
     gene            complement(7483..8850)
                     /locus_tag="VGB76_12180"
     CDS             complement(7483..8850)
                     /locus_tag="VGB76_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY0099699.1"
                     /translation="MDYHENILGLIGNTPLVKLNHLTKGIKATVLAKMENLNPGYSVK
                     DRIGISMIEAAEREGKLKPGGTIVEATSGNTGIGLALAAAVRGYRCTFVMTDKASVEK
                     VRYLKALGADVVVVPSSAKPGTPDHYVSTAQRIASETPNSFYPNQYAHPANPEAHYRT
                     TGPEIWKQTEGKITHFVSGLGTGGSISGTGRFLKEQSPDIKIIGADPYGSVFKTYKET
                     GQLIQATPYLVEGIGQEIIPDNVHLKYVDEIINVTDRESFELSRQLGRMEGIFCGGST
                     GTNLAAALKVARGLDENGLVVFIVCDTGEHYLSKHHSDEWMKEKRLIEPQKITAGLIS
                     GTKNQHSPGTLVSVVPTERVSDVLAKMDELGLSYIPVIEEGKSVGSLRENRMLAKVLG
                     NRELMNAPVSELMEASFPLIDVDASVSEVTKRLQKSPAILVEEYGRISGIITRHDLLD
                     APHPG"
     gene            9199..10290
                     /locus_tag="VGB76_12185"
     CDS             9199..10290
                     /locus_tag="VGB76_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099700.1"
                     /translation="MSNTRKPRVNPLSRRPRQQRLVMAVRGGAGVGKSHFVRSMVEAG
                     LGRLCIFDVERKTRLLSGNGVAFDGLEIEHPDELPEFIDWALSGEGQEQNYGCFALDS
                     WASYFSAKHSEMVAAVRERTGDPLAQPSAEELAADQMILQGVLRRLCIESGKSVVIVD
                     QIPAKGKESKEDNEVGRVLPMTASGLEYFVDIMVEVSLQERDGEVVRVFQIVKSNSPA
                     FEVGFELTGAVTFSDFLNHMKRESGTDLPMETPRAQSVPETLEEKPSPLSLVPPTLTI
                     QELITKAEQHGFKVADIVTGAKLYHNQTNIYRLTPEQIADLDRRITARIEKMNNNAAD
                     DKAGAAKQGANVNETNARAAGAARNIKRA"
     gene            complement(10451..11572)
                     /locus_tag="VGB76_12190"
     CDS             complement(10451..11572)
                     /locus_tag="VGB76_12190"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0099701.1"
                     /translation="MNLSELPTPALVLDVARVRRNAERMSARVKSMGARLRPHIKTHK
                     CVQIARLQTTAESAALTVSTLAEAVWFAARGFTDLTYAVPIEPGKFAAAIELTRECER
                     LALLTDDLETTKQLEAAARAANVRLEMFLKVDCGYHRCGVEPHTKEAYEIARRIQAAS
                     HLHFAGILTHAGHSYHSRTPEELQRIARRERDVMVEFAATLRHEDIPVPVVSIGSTPT
                     ISTVEHLEGVDEARPGNYIFFDAFQATLGSCRFEDCALTVLSAVVHRDASRRKVVIDA
                     GAVALSKDRGAFEFDETCGYGRVLDVEGRELNLRVASLSQEHGMIEVTDAALFEKLKV
                     GTRLRILANHSCLTAAQHPHYNVLEGGRIVDRWQIQRGW"
     gene            11801..12772
                     /locus_tag="VGB76_12195"
     CDS             11801..12772
                     /locus_tag="VGB76_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099702.1"
                     /translation="MTSRLWHMNADFEMELAADSPGSYRRPQTFQALNRRLAPHLLWL
                     ASPGDALLLEESWPETLAREAERRGVELILHNKATPPQPDRIFTPWGWTPSVVRLGES
                     LSARLPTVSLETVRRVNSKLWSHALEQELGIAMRGACVAADFAELQEAVASACPRADD
                     KWVIKSPFGFAARERVLGRGARLEGAQATWTRRQFARGVKLIFQPWLERVREYGVTFE
                     IEAKGEINVKGISDLQTNGAGTGTGYLLGRRIGAERVAELQRLAQVVGMRLFREGYTG
                     AAGLDALEHTGGLHPLLEINARYTMGFVALAIERALNPPTPTLWELK"
     gene            13101..13301
                     /locus_tag="VGB76_12200"
     CDS             13101..13301
                     /locus_tag="VGB76_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099703.1"
                     /translation="MENNQPNDLIAVAEAQRLLGVSHAKIAQLIREGALRHFPNPLDK
                     RVKLISQTEALALKPQRVDKAA"
     gene            13298..>13643
                     /locus_tag="VGB76_12205"
     CDS             13298..>13643
                     /locus_tag="VGB76_12205"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0099704.1"
                     /translation="MKTIAIANHKGGCAKTTVALNIAVTLATTGSRVLAVDLDPQGNL
                     SASLGIDLEELEDSRHTSHRLMLDERGDFSTYTANVRPRLDVLPSCLDDDAEALLDGQ
                     AVSRELLLKQKLA"
BASE COUNT         2657 a         3936 c         4134 g         2916 t
ORIGIN      
        1 cgtagccctg ctcctcaaga attacctgga gcgcgcccgt gatgctgctc tcgtcgtcaa
       61 tgaccaggat tttgatccat gctcgcatct tctgcgtact cacttttcgg ctgggagttc
      121 gatgatgatt tgcgtccctg cctcccggcg gctctcgatg tgaattgccc cgcgatgtag
      181 ctcgatcacc tttttggcgt agggcatgcc taagcccagt ccttgagatt tcgtcgtgta
      241 gaaaggctcg aagacttttt cgatctgttc gggagtcatg ccgtggcctg tgtcggcgat
      301 ggtcagttgg ctcgtgccgt tcacgcggtt ggtcttgacg gtcaggcggc ctccgtccgg
      361 catggattga atggcgttga gcgtcaggtt gataagcgcg gaggtgatgg aagtttcgtc
      421 gagcagcgtc ggcacttcgg cgtccgcgag ttcgaggcgt tcgtcgatgc ggtgtgcgtc
      481 gatctgcggt tgcagcagtt ggcagacttt gcgcacgacg aggttgagat cgacggggcg
      541 cggcgagagc gagacggggc gcgcaaaact gagaatctgt tcggtcgtac cgacgagatg
      601 attgatgctg ttgatgatgc ggtcgcagag tgcgacttcg ctctcggtca gtttgctgcc
      661 gagtttgttc ttcaagtgca tggcgtagag cagcagcccg gcgagcggat ttttgacctc
      721 gtgcgccacc tgcgcggcga cgcgcccgag cgcggagatc ttctcgttgt ggaagagttg
      781 acgctgcatc tcctccttct ccgcttccag acgcttgcgg atcgaccaat cgcgcacgat
      841 gaggacgcgc agacgctcct cgtccatcga gagcgtgctg cacgacacgg agacgggcag
      901 cgccagaccc tttgaagtat gaatggataa ctctctatcg gtgcagttta gatcgttcaa
      961 agcgatggcg agcgtgtcgc cgtctacgtc tccctgcgtc cgccaatcga gttcgttgat
     1021 gttcttgccg agcaattcct gcgggcgaca gccgaagagt tcgcaaaact tatcgttgac
     1081 ttcgatgacg ctgcgattcg cggcggagac gatcacgatg ccgtcgttgg cgcgttcgaa
     1141 gagggcgcga taacgctcct cgctggcgac gagtttgcgt tgctggcgac tgaccagacg
     1201 gagcagcaac gagacgacga tcaagtccac cgtgactgag atgaagaagt gctcgaacct
     1261 ctgctgcgtt ctccatctgg cgacgctgtt ggccgcgccc ggataatatg cctcaatcac
     1321 cgaatagaag ggcgccatca gcaccttctc ccacaacaaa accaccacgc cggttaataa
     1381 gaccgtgagt gcggtgaaga agagaacgag aagtaagttg cgtctctttt tcatgtgcgc
     1441 ctcccgcctt aaatttgtgt tgcgcccgcg ccggtcggat cgataaagag ctagcgggtc
     1501 gtcgagtatg tgtatgaacc gtctcaggct tctgtttcgc gtgccgatca cacgtttccg
     1561 cacacagggc cgagcagtcc tctgtaaatt gagggggtta atattgaaga atatagcttg
     1621 tcgaggctaa attctacggc cttgtgaagg ggaaaactga acgggactgc tgaaaaatac
     1681 tcccggcggt tgggcttgtc aagaaacttg caccggcctg tcaagaaact tgcagcaacc
     1741 gtttaagaca ttgcatcgaa cagtttcagt ttgcgacggc gggcggcggc gagtttgtcc
     1801 agaaactcct cgcgcgggat ggtgcgcgcg ccgagggctt tcatgtgcgg cgtcatcact
     1861 tgaatatcga gccaatcgag gccgcgcagt tgcaggtttt cgatgaggtg gagcagcgcg
     1921 agtttcgagg cgttcgggcg caggtagaac atgctctcgc ccgcaaacgc gccgcccgca
     1981 tcgactccgt aaagcccgcc gaccagcgca tcgccctccc acgcttcgac gctgtgcgcg
     2041 tggccgagtt cgtgcaggcg gcaataggct tcgagcatct gcggcgtgat ccaagtgccc
     2101 tgttcatcgg ggcgcgcgac gcccgcgcaa gcgagaatga cggcgcgaaa cgccgcgtcg
     2161 atggtgagac gaaagggggc gcgtcggcgc actttggcga ggctgcgcgg aacttttaaa
     2221 tccttgaact cgatgatcgc gcgctccagc ggagagaacc aggtcagagg ccagccctca
     2281 atcggccagg gaaaaatccc tctctcgtag gcgcgcagca ggttgagcgt gtgtagattt
     2341 ccgcccacgg cgacgatgtc accgtaaggc gcgtctcgcg gatcggggaa acggatcagt
     2401 tccgcgtccc gcttgaaaaa ctctgcgggg tggcgatatg aaatgtctgt ctccgacata
     2461 acgcgagtgc gaattcatca gagcgtttca acttctttgc gccttgagtg tgctgcttcg
     2521 taaaagcaag gggcgtgaag gactgaatgc ctgctctgat ggctcaattc ccttttgctt
     2581 cgttaacttc aagaccgggg aagacgaggg tgagttcttc gttcgcaacg cggacggaga
     2641 cttcgccgcc cgacgcgagc gacgtgccga agagcaactc ttccgcgagc ggttccttga
     2701 tcttcgactg gatcaggcgt cccatcgggc gcgccccgaa cagacggtca aacccgcgcg
     2761 aggccagcca cgagcgcgcc gcttcgtcca gcacgacgcg cacgttccgc tccgcgagtt
     2821 gcgcgttcag ttctttaata aacttgtcca caaccttttc gatcacttca aaactgagcg
     2881 gcgagaaagc gatccacgcg tcgaggcgat tgcgaaactc cgggctgaac gtgcgttcaa
     2941 tcgcgccgcg cccctgtcct ttgcccgaag tgttcgactg aaagccgatg ccgcccgcgc
     3001 tcaactcctg cgcgcccgcg tttgtcgtca tgatcaaaat gacgttgcga aagtctgcct
     3061 tcttgccgtt gttgtcggtg agcgtggcgt gatccatcac ctgcaagaga atgttgaaaa
     3121 ggttcgggtg cgctttctcg atctcgtcga ggacgagcac ggcgtaggga gttttgttga
     3181 tcgcgtctgt gagcaggccg ccctgatcga agccgacata gccgggcggc gcgccgatga
     3241 gacgagaaac cgtgtgcgct tcggcgtact cgctcatgtc gaagcgtatg aactcgatgc
     3301 cgagcgcgtg tgcgagttgt ttagccagtt cggtcttgcc cacccccgta ggacctgaga
     3361 agagaaacga gccgacaggc ttttcgggat tgcccagacc ggagcgcgac aacttgatcg
     3421 cgttcgtgat cccctcaatc gcttgatcct gtccgtagat gaccgcctgc aattctcctt
     3481 tcaaatcgcg caggcgatcc cgctccgaac cggcgaccgt cttcggcgga atcttcgcca
     3541 tgcgcgcgag cacgatctcg atctcggcag tgccgaccgt ctccgggcgt tcttccttcg
     3601 gcaacaactt catcgccgcg cccgcttcgt ccatcacgtc aatcgcttta tcgggcagca
     3661 ggcggtcgtg cagatacttg gcggcgagtt cggcggcgag ttggatcgct tcgggcgcat
     3721 aggtcacgcc gtgatgcttt tcgtagtagc tcttgaggcc gtgcagaatc tcgaccgtct
     3781 cggcgaggct cggctcgccg atctcgatct tctggaaacg tcgcgcgagc gcgcggtcgc
     3841 gttcaaacgc gctcttgtac tcggcgtaag tcgtcgaacc gatgcagcgc agttcgccgg
     3901 aagcgagcgc gggcttgatg atgttggagg cgtccatcga accgcccgac accgcgcccg
     3961 cgccgacgat ggtatgaatc tcgtctatga agaggatcgc ggcgggaatc ttctttaacg
     4021 cgttgatgat ggctttgaaa cgctgctcaa actcgcccct gtatttcgtg cccgccaaga
     4081 ccgcgcccat gtcgagcgca tacaccactg cgtcgcgcaa cacttcgggc acgtcgccct
     4141 gttgaatctt caaggccagc ccctcggcaa tcgccgtttt cccgacgccc ggttcgccca
     4201 cgtagatcgg gttgttcttg cgccggcgac agagaatttg aatcgtgcgc agaacttcca
     4261 cgctgcgccc gatgagcggg tcaatgaagc cttcgcgcgc gcgttcgttg aggttgacgg
     4321 tgaaggcggc gagcggatcg cgttcctgtc cggcgtcggc ttcgccttcg gtcgcgccgt
     4381 caaactcgtt gcccgccggt tcgtagccgc ccgcgagctt ggagatgccg tgcgcgatga
     4441 agttgagcac gtcgagacgc gtgatgccct gctccttgag caggtagacg gcgtaggagc
     4501 gttcggcctg aaagagcgcg gcgaggatgt tgccgccgtc gatctctttc tgcccgctgc
     4561 cctgcgcctg tagcatcgcg tactggagcg tgcgcgtgaa ggtgtcggtc tgctccggaa
     4621 tgtgcgtcgt atttctggtg accaactcga tgcgctcgtc gaagtagagt tcgaggtcgc
     4681 ggcgcagcgc gtccgtgtcg ccgccgcaat ggcgtatgac gttgctcgca gtgctgtctg
     4741 aaagcaacgc gagcaaaagg tgttcgagcg tgacgtattc atgtctccgc tcgatggcct
     4801 cttcgacggc tgcgctcaaa gtcgcttgta gctctctcgt aatcataaaa aaagcagggg
     4861 tcggaagtta gaggcgaagg tgtgcaagaa tcagttcgtc tctttaaccg cactctcacg
     4921 ccggaactct aaacactcgt ttcttcaatc gtgcagagca gcggaaactc gttcgcacgc
     4981 gccaactccg tcaccttcga caccttcatc tccgcgatct cgtaggtata gacaccggcc
     5041 acgccgacgc cttcaaggtg cacttggagc ataatgcgga gggcgtcaac ctcggatcgc
     5101 tgaaagacat tttgcaacac gtagacgaca aactccatcg tcgtgaagtc gtcgttgtgc
     5161 aggacgactt gatagagcgg cggtttcttg agccgcgtct cgctctccgt gacgaccgct
     5221 tcgtccgtgt ccgaatcgaa tcttgacata aatgccgttt ctcgcgcgca gccgttctgc
     5281 gaccgcacgt tgacaggata ccaacatcac acgcgggacg gtagcgtgca gggcagaacg
     5341 gcagtggaca tgataatcgc cgcccctttt ctaccctgac acgtaagccg gatagaaaaa
     5401 ggggcgacgc tttgatcggc gtcgcctctt tttatgttat cgggggtttg cccgtaagcg
     5461 tgtgcttggc cgccatcggt tcaactgcaa accgggttca gcggtgaaaa ttactcgctg
     5521 tgctggacgg ggttgatgcc tttgctggcc tgactcgcgg tgcgttcact atgttggaca
     5581 ggattgatgc ccttactcgc atttgaattg agtatcgtga gggcaagtaa cagcgcggcc
     5641 aatgtaacta tagtagacat aagttttcct tttcagatcg tggacgtgag gctaatgaga
     5701 cattagcgcg gcgaaagata tagggaaagg cggtgtgtgt caatagttat ccggctatgt
     5761 gtccgggtgg aatttgatgg atgcggcgac gagccagacg agcctgattt cctcactttc
     5821 ccttgcttcc gcgctgccct tcgagcgcgc tcaacagcag acgcgtgaga cgccgcactt
     5881 ctttcgcgtc cgacggcggc gcgtaatcgc cgcgcacgtg caactccaac cccggctcga
     5941 tctccacgcg ttcccacgtg gccggactcg aaggcgcagc ttgacgctgt gtggctaaat
     6001 tcggagttgg tttggcgagc ggtgcgtcgg cgggactggc gaagttttgc tttcgagacg
     6061 gggtggaggc ggcggcgggc gaggtcgatc tggtgagcaa cttttccaga tagctgtggg
     6121 cttcgttctt cgccccggct ttggcgtcga ggccgagaag tcgttccaac tcccgttcgc
     6181 ttcgcccgtc aaccaattct ttgatcttgc gaatcggcaa atgctccgat tgcagtttct
     6241 tgacgacgag caactgtagg agatgcacgt agccgaagac ggaggccgtg ccctgcttat
     6301 ccgaggcggg cgagagcaat ccttcggcga gatagtaacg gagcgtgcgt tcgtctggca
     6361 tctcgctgac cgtgccgcga tcctgcgtcg tgccgatctc cgccagaatc ctcgccgcct
     6421 gtttcgccag ttcgacgacg ccgatatatt tttgactccg tatcttctcc agttgtgtgc
     6481 tcatggcggc taacaatagt tcaagcagcc atgccggtca acagcattta ctgtcactac
     6541 aggagacgga tggctgagga gcgcggcgca ggtcttcatg gtttatgatg aagcggagtt
     6601 tttcatgtct actatttcag cgggagcgga aagttgacga agcgtggcaa gtatctgcgc
     6661 gcctcggagg tggccgagta cgtctactgc gcgcgtgcct ggcggctgcg cgtggacggg
     6721 cgcgaaccgg cttcaggcca cgcgcaacgc gcggcgggcg aagcgtggca tctggcgcac
     6781 ggtgcggcgg tcgtgcgggc gcggaggatg cgcttgctcg ccgggttcgc tttcgcgctc
     6841 gcgctcatcg tcggcctctt aattttattg ctcggatggc gttgagttta ctctatctgg
     6901 ctggcatcgt tttgttgctc gtcgcgctcg gcgcgtggct ggcgtcgcgt cgtggtgcgc
     6961 ggcaggcgga tttgccggaa ggtcgaatca tctatagcga tacgggtgcg cccgtgggac
     7021 gcatcgctcc ggtgacgttg agcgagcgcg gcgagcgaca ggagaagcca ctgctttcgc
     7081 atcgtcatgc gctcgtcggg cgacccgatt atctggtgca gacggaggga gggatcattc
     7141 cggttgaggc caaatccgcg aactgcccgg cgagcggcgt gccctacgag tcgcacttga
     7201 tgcaactcgc ctgctactgc ctgctcgtcg aagaggcgac gggcgcgacc gtgcccttcg
     7261 gcgtgatacg ttaccgcgac cgccagttgc gcgtggacta cacggacgag ttgcgcgaga
     7321 gcttgctcga actgctcgcg gagatgcgcg aagcgcggcg cgcgggcgaa gtgcatcgca
     7381 gccacgaaga ggcggcgcgc tgtgcggcct gtagctacag agaaatttgc gtcgaatcgc
     7441 tggcggattg aagctgcggg ttaaagctgc acttcgagtc ttctatccgg ggtgcggcgc
     7501 gtcgagcaga tcgtggcgcg tgatgatccc tgagattctg ccgtactctt ccaccagaat
     7561 cgccggactc ttttgcagac gcttcgtgac ctcgcttacg ctcgcatcca cgtcaatgag
     7621 cggaaagctc gcttccatca attcgctcac aggggcgttc atcaactccc ggttgccgag
     7681 gactttcgcc agcatacgat tctcacgaag cgacccgacg gacttccctt cctcgataac
     7741 cggaatgtaa gacagcccga gttcgtccat tttcgcgagc acgtcggaga cgcgttcagt
     7801 tggaacgacc gagacgagag ttcccggcga gtgctggttc ttcgttccgc tgatgagtcc
     7861 ggcggtgatc ttttgcggtt cgatgagacg cttttctttc atccattcgt ccgaatggtg
     7921 cttcgacaaa tagtgctcgc ccgtgtcgca gacgatgaag acgacgaggc cgttttcgtc
     7981 caagccccgc gcgactttga gcgcggcagc gagattcgtg cccgtcgaac cgccgcagaa
     8041 aattccttcc atgcgcccga gttggcgcga cagttcaaac gattcgcggt cggtgacgtt
     8101 gatgatctcg tccacgtatt tgagatggac gttatccgga atgatctcct gtccgatgcc
     8161 ttcgacgagg tacggagtcg cctgtatgag ctgccccgtc tccttgtaag tcttgaagac
     8221 cgagccgtag gggtctgcgc cgatgatctt gatgtcggga ctctgctctt tgaggaaacg
     8281 acccgtgccg gagatcgaac cgcccgtgcc cagacctgag acgaagtgcg tgatcttgcc
     8341 ttccgtctgc ttccagattt cgggtcccgt cgtgcggtag tgcgcctccg ggttcgcggg
     8401 gtgcgcgtac tggttcgggt agaaggagtt cggcgtctcg ctggcgatgc gctgcgcggt
     8461 cgagacgtag tgatcgggcg tgcccggctt ggccgaagag ggcacgacca ccacgtctgc
     8521 gccgagcgcc ttgagataac gcaccttctc gaccgatgct ttgtccgtca tcacgaacgt
     8581 gcagcgatag cctctgaccg ccgccgcgag cgcgaggccg atgcctgtgt tgccggaagt
     8641 cgcttcgaca atcgtgccgc cgggtttgag tttcccttcg cgttccgccg cttcgatcat
     8701 cgagatgccg atgcggtctt tgacggaata gccgggattg agattctcca tcttggcaag
     8761 caccgtcgcc ttgatgcctt tggtgagatg gttgagtttg acgagcggcg tgttgccgat
     8821 aaggccgagt atattttcgt ggtagtccat aaactctctt ctgctgcgga attgaaaccg
     8881 gcggcaagcc gcgccgtcgc tggttgaatt gaacactcta taaaaacttt tcttccacgc
     8941 tttgtcaaag ctcacgacca ccggacgcgc cgagacgact gttgcatgtc cggtcagcac
     9001 ttgtttcatc tatgaaatat tgttgcactc gcgcctgaaa catgctacag tgccagcgtt
     9061 ttcgagagaa acgctccagt gctgcttgaa gtttttcgtg aggcactccc gagagcggtc
     9121 gctgtaaaaa agtttttggc gggcgaacac gcacaaaccg tcaccctaaa caagaaacac
     9181 tgtaaggagc atctagtgat gagcaacaca agaaaaccga gagttaatcc gctgagccgt
     9241 cgtccgcgtc aacagcgtct ggtgatggcg gtgcgcgggg gcgcgggcgt gggcaagagc
     9301 catttcgtgc gcagcatggt cgaggcgggt ttgggtcgcc tctgcatctt cgacgtggaa
     9361 cgcaagacgc gactcctcag cggcaacggc gttgccttcg acggcctcga aatcgagcac
     9421 cctgacgaac tgcccgaatt cattgattgg gcgctctccg gcgaagggca ggagcagaac
     9481 tacggttgtt tcgcgctcga ttcatgggcg agctatttca gcgcaaagca cagcgagatg
     9541 gtggcggcgg tgcgcgaacg cacgggcgac ccgctcgcgc agccttccgc cgaagaactc
     9601 gccgccgacc agatgatcct gcaaggagtc ttacgacggt tgtgcatcga gagcggaaag
     9661 tcggtcgtga tcgtggatca gattccggcg aaggggaaag agtcgaagga ggataacgaa
     9721 gtcgggcgcg tgttgccgat gacggcgagc gggttggaat acttcgtgga catcatggtc
     9781 gaagtctcgc tgcaagagcg cgacggcgaa gtggtgcgcg tcttccagat cgtgaagagc
     9841 aacagccccg cttttgaggt tggcttcgag ttgacgggcg cggtcacctt ctcggatttt
     9901 ctcaaccaca tgaagcgcga gtccggcacg gacctgccga tggagacgcc gcgcgcacag
     9961 agcgtgccgg agacgctcga agagaagcct tcgccgctct cgctcgtgcc gcccactttg
    10021 acgattcagg agttgatcac gaaggccgag cagcatggtt tcaaagtcgc agacattgtg
    10081 acgggcgcga agctctatca caaccagacc aacatctatc gcctcacgcc cgaacagatc
    10141 gcagacctcg accgccgcat caccgcgcgc atcgagaaga tgaacaacaa cgctgcggac
    10201 gataaagcgg gcgcggccaa acagggcgca aacgtcaacg agacgaacgc cagagccgcg
    10261 ggcgcagcgc gcaacatcaa acgcgcttaa gcgcatttcg aacgcgcgca caacctccgc
    10321 ttttgcagaa aatttgagca caggaacgag agcctccaaa ctctcgttcc tgtgcttttc
    10381 aatttgggac tctgattttg cgtcgaggca ttttttgacg tttccgggat aaagtttttc
    10441 gagtggagcg ttaccagccg cgttgaattt gccagcggtc aacgatgcgc ccgccttcga
    10501 gcacgttgta atgtggatgc tgggcggcgg tcaggcacga atggttggcg aggatgcgca
    10561 ggcgcgtgcc gaccttcaac ttctcgaata gtgcggcgtc ggtcacttcg atcatgccgt
    10621 gctcctgcga aagcgacgcg acgcgcagat tcagttcgcg cccttcgaca tcgagcacgc
    10681 gcccgtagcc gcacgtctca tcaaactcaa acgcgccgcg atctttcgag agcgcgacgg
    10741 ctcccgcgtc aatcaccact ttgcggcggc tcgcatcccg gtgcacgacg gcggagagca
    10801 ccgtcaaagc gcaatcctca aaacgacaac tgccgagcgt ggcttgaaag gcatcgaaga
    10861 aaatatagtt tcccggtcgc gcttcatcta cgccttcgag atgttcgacg gttgagatgg
    10921 tcggagtcga accgatgctg acgacgggca cgggaatgtc ctcgtgacga agcgtggcgg
    10981 caaattcgac catcacatcg cgctcgcgcc gggcgatgcg ctgcaactct tcgggggtgc
    11041 gactgtgata cgaatgtccg gcgtgcgtca ggatacctgc gaaatgtaga tgcgaagccg
    11101 cttggatgcg gcgcgcaatc tcgtaggctt ccttcgtgtg cggttcgacg ccgcaacgat
    11161 gatagccgca gtccactttc aaaaacattt ccagacgcac attcgccgcg cgtgccgccg
    11221 cttcaagttg cttcgttgtt tcgagatcgt ccgtcagcag ggcgagccgc tcacactcgc
    11281 gcgtgagttc gatggcggcg gcaaacttcc ccggctcaat cggcacagcg taagtcaggt
    11341 ctgtgaaacc gcgtgcggcg aaccacactg cctcggccag tgtggacacg gtgagcgcgg
    11401 cggactcggc tgtcgtttgc agccgcgcaa tctgtacgca cttatgcgtc ttgatgtgcg
    11461 ggcgcagacg tgcgcccata cttttgaccc gcgcagacat gcgctctgcg tttcgtcgca
    11521 cgcgcgccac gtcgagcaca agcgcgggtg tcggcagttc tgataaattc atacgcgttg
    11581 gatgttgccg caaaagccgc cttgagggca atcaaaatta tgtgtgtgtg taaacaggct
    11641 gccgccttcc tttgcgacgc cgctcgaaat ttgtctcacg ccgagccgca caggaagaga
    11701 ggaagcattg aatagtaagt ccccggtttg aacaaagttt gaggttgacg cgcacagcgc
    11761 gctctcttct tttagtgatc tctgattcga tgtcaacgaa atgacttctc gactctggca
    11821 catgaacgcc gattttgaaa tggagttggc tgccgacagc cccggctctt accggcggcc
    11881 acagaccttt caggctttga atcgtcggct cgccccgcac ctgctctggc tcgcgtcccc
    11941 cggcgatgcg ctgctcctcg aagagagttg gcctgaaact ctcgcgcgcg aagcggagcg
    12001 gcgcggcgtc gaactaatcc tgcataataa ggcgacgcca ccacaacccg acagaatctt
    12061 tacgccttgg ggctggacgc cgagcgtcgt ccggctcggc gagagtttaa gcgcacgcct
    12121 tcccactgtc tcgctggaga cggtgcggcg cgtcaactcc aaactttgga gtcatgcgct
    12181 ggaacaggaa ctcggcatcg cgatgcgcgg cgcatgtgtc gccgcagact tcgccgagtt
    12241 gcaggaggcg gtcgccagcg cgtgtcctcg tgcggatgat aaatgggtga tcaaaagccc
    12301 tttcggattc gcggcgcgag aacgcgtgct cggtcgaggc gcgcgcctcg aaggcgcaca
    12361 ggcgacatgg acgcgtcgcc agtttgccag aggcgtaaaa cttatttttc aaccgtggct
    12421 tgagcgagtg cgcgagtacg gcgtgacatt tgagatcgaa gcgaaagggg agatcaacgt
    12481 caaaggcatc agcgatctgc aaacgaacgg cgcgggcaca ggcacgggtt acctgctcgg
    12541 tcgccggatc ggggcagaac gcgtggccga actccaacgt ctcgcgcagg tcgtcggcat
    12601 gcgcctcttc cgcgaaggtt atacgggagc agcgggcttg gacgcgcttg agcacacggg
    12661 cggcttgcac cccttgcttg aaattaacgc acgctatacg atgggcttcg tcgcactcgc
    12721 catcgaacgc gccctcaacc cgccgacgcc gactctctgg gaactgaaat aactatccgg
    12781 ctgccctggc gcgaaccgcc gaagcccgcc agttttccag aacagaacgt cgccgttgtc
    12841 agccccgccg aacgccattc ttcaaacggc gcagaactac tcttcgcacg aacgcctcgc
    12901 agtcgcttcg catttcttgg agagaagttt atacgcgaaa acattgattg tctgccgcac
    12961 agtccttccc ggcacgcatt cgccctcttc aattcccttc ccgctatttt gaaactttat
    13021 ttactatttt gagctaagtt gaggtatatt gctcgacctt gaggattact gttcaacttc
    13081 aagagtatga aggagggcaa atggaaaaca atcaaccaaa cgacttgatc gcggtcgctg
    13141 aagcgcagcg gctgctcggc gtcagccatg ccaaaatagc gcaactcatc agagaaggcg
    13201 cgctcagaca ttttcccaac cccctggaca agcgggtcaa actcatcagc cagaccgaag
    13261 ctctcgcact caaaccgcaa cgggtggata aagcagcatg aaaactatcg ccatcgcgaa
    13321 tcacaaaggc gggtgcgcga aaacgaccgt cgcgctcaac atcgcggtta cgctggcgac
    13381 cacaggatcg cgtgtgttgg ccgtcgatct cgacccgcag ggaaatctct ccgcgtcgct
    13441 cggcatcgac ctcgaagagt tggaggattc acgccacacc tcgcaccgcc tcatgctcga
    13501 cgagcgcggc gatttctcta cctacacggc caacgtccgc ccacggctcg acgtgttgcc
    13561 gtcctgcctc gacgacgacg ccgaagcgct ccttgacggt caggccgtgt cgcgcgaact
    13621 gttgttaaaa cagaaattag ctc
//
LOCUS       DASWNS010000349         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_168028, whole genome shotgun sequence.
ACCESSION   DASWNS010000349 DASWNS010000000
VERSION     DASWNS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168028"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VGB76_12210"
     CDS             complement(<1..903)
                     /locus_tag="VGB76_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY0099705.1"
                     /translation="MKSSSLDGNKLRRRFFLLASICVLCFSACGKRRPPLPPVENVPQ
                     RTELLSGVQRGNQVILSWPAPRRNADDGSVQSIRRIDIYRVAERTDDPLPLTEEEFST
                     RATLIGSVPFSSIKNAPEDLTYEDTLTLTEPVRLRYALRYVNATGQRASFSNFLLIEP
                     ATRVSQPPVLLSVSNESENVVRVRWQTPPANVDNSTPLNLLGYNVYRTDRSQTEPAQT
                     PLNSSVLTATSFDDQTFKFGEEYVYIVRAVSLGTGGAQVESLNSNAQSIAPRDTFPPS
                     APEKISVAPAPRRLSLFFPANPERD"
     gene            complement(938..2380)
                     /gene="hslU"
                     /locus_tag="VGB76_12215"
     CDS             complement(938..2380)
                     /gene="hslU"
                     /locus_tag="VGB76_12215"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY0099706.1"
                     /translation="MVIYLSGEVDEEARLDDMTPRQIVQQLDKHVVGQHAAKRAVAIA
                     LRNRVRRQKLPPDMAADVMPKNIIMIGTTGVGKTEIARRLARMSSSPFLKVEASKFTE
                     VGYVGRDVESMIRDLTEIAVEMLRAEKAEEISDKAELNVEERILDLLLPPKPSSNFYS
                     FDDEENSGSSSPSDGGASGAPLRDDQFQRTREKLREQLRAGKLENRMVELEVREKNFP
                     TIEVAGPQGVEEMGINMKDMLGNLFQGRTKRRRMRVDEAMEYLLQEEEERLIDMDTVA
                     RAALERVESSGIIFLDEIDKIAGRESGGGGPDVSREGVQRDILPIVEGTTVNTRYGMV
                     RTDHILFIAAGAFHVSKPSDLIPELQGRFPIRVELESLTIEDFKRILTEPKNSLVKQY
                     RALLETEGVELEFTGDAIEAIAQFAGRVNEQTENIGARRLHTIMEKVLDVISFEGPDL
                     EPKRQVIDAAYVSNTLQEIVKDQDLSRYIL"
     gene            2464..>2914
                     /locus_tag="VGB76_12220"
     CDS             2464..>2914
                     /locus_tag="VGB76_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099707.1"
                     /translation="MTLDQLFESFIREKQYIGNCTPRTITFFRQSFKTFRRIVTEDEM
                     TRQTTTDFVIGARQKGMSVGCLNAYIKGVNSFLEWLHQNEITKERLKIKLLKQEKKVL
                     RSFTEDELRRVLSFKPKTFGERRIFALTLTLIDSGGRIDEFLSLTRQQ"
BASE COUNT          604 a          808 c          870 g          632 t
ORIGIN      
        1 gtcgcgttcg ggattggcgg ggaagaagag cgagagacgg cgcggcgcgg gggcgacgga
       61 gatcttttcc ggcgcggacg gggggaacgt atcacgcggc gcgatgctct gcgcgtttga
      121 attgagactc tcgacctgcg ccccgcccgt gccgagcgag actgcgcgca cgatgtagac
      181 gtactcttcg ccgaatttaa aagtctgatc gtcgaaggag gtcgcggtca agaccgaact
      241 gttgagcggc gtctgcgcgg gttcggtctg cgagcggtct gtgcgataga cgttgtagcc
      301 gagcaggttg aggggcgtcg agttatccac gttggcgggt ggcgtctgcc agcggacgcg
      361 caccacgttc tcgctctcgt tcgagacgct caggagcacg ggcggctggc tcacgcgcgt
      421 cgccggttcg atcagcagga agttcgagaa cgaagcgcgc tgccccgtgg cgttgacgta
      481 gcggagcgcg taacgcaggc gcacgggttc cgtgagcgtg agcgtgtctt cgtaagttaa
      541 atcctccggg gcgtttttaa tcgaagagaa ggggacggag ccgatgagcg tggcgcgcgt
      601 ggaaaattct tcctccgtca gaggcagagg gtcgtccgtg cgctctgcga cacgatagat
      661 gtctatacgg cggatgctct gcacgctgcc gtcgtctgcg ttgcggcgcg gcgcaggcca
      721 actcagaatg acctggttgc cgcgctgcac gcctgagagc aactccgtgc gctgcgggac
      781 gttctcgacg ggcggcagcg gcgggcggcg tttgccgcag gccgaaaaac agaggacgca
      841 gatagacgcg agcaggaaga agcggcggcg tagtttgttc ccgtcgagac ttgaagattt
      901 catgcgcgga tgccgtgtgc agacaaaatc tgcgtgctta caaaatgtag cggctgaggt
      961 cttggtcttt gacgatctct tgcagcgtat tggagacgta agcggcgtca atcacctgac
     1021 gcttcggttc gaggtcggga ccttcaaaac tgatcacgtc caagaccttc tccatgatcg
     1081 tgtgcaggcg acgcgcgccg atgttctccg tctgctcgtt gacgcgcccg gcaaactgcg
     1141 cgatggcttc gatggcgtcg cccgtaaact ccagttcgac gccttccgtc tccagcagcg
     1201 cgcggtattg tttgacgagc gaatttttcg gttcggttaa aatccgtttg aagtcttcaa
     1261 tcgtcagcga ttcgagttcg acgcggatgg gaaaacggcc ttgcagttcg ggaatcaagt
     1321 ccgaaggctt cgagacgtga aacgcgccgg cggcgatgaa gaggatatga tccgtgcgca
     1381 ccatgccgta acgcgtgttg acggtcgtgc cttcgacgat ggggagaatg tcgcgctgca
     1441 cgccctcgcg cgacacgtcc ggacctccgc cgcccgactc gcgcccggcg atcttgtcga
     1501 tctcgtccag gaagatgatg ccggaggatt ccacgcgttc gagggcggcg cgcgccaccg
     1561 tgtccatgtc tatgagtcgc tcctcctctt cctgcaagag atactccatc gcttcgtcta
     1621 cgcgcatgcg gcgacgtttc gtgcgtccct gaaagagatt gccgagcatg tctttcatgt
     1681 tgatgcccat ctcttcgacg ccctgcggcc ctgcgacttc gatggtcggg aaattcttct
     1741 cgcgcacttc caactcgacc atgcgattct ccaatttccc tgcgcgaagc tgctctctca
     1801 gtttctcacg cgtgcgctga aactggtcgt cgcgcagggg cgcgcccgac gcgccgccgt
     1861 cggacggaga actgctgccg ctgttctctt catcatcgaa actgtaaaag ttagaagaag
     1921 gtttcggggg cagcagcagg tcgagtatgc gttcttcgac gttgagttcc gctttgtctg
     1981 aaatctcttc ggccttctcc gcgcgcagca tctcgactgc gatctccgtg agatcgcgga
     2041 tcatgctctc cacatcgcgc cccacgtagc cgacctccgt gaacttcgag gcttcgactt
     2101 tgagaaaagg cgaactcgac atgcgcgcga ggcgacgcgc gatctctgtc ttgccgacgc
     2161 ccgtggtacc gatcatgatg atgtttttcg gcatcacgtc ggcggccatg tcgggcggga
     2221 gcttctgtcg ccggacacga ttgcgcaggg cgattgccac ggcgcgcttc gcagcatgtt
     2281 gcccgacgac gtgcttgtcc aattgctgca cgatctgtcg cggcgtcata tcgtcgagcc
     2341 gcgcctcttc gtcaacttcg cctgagagat aaataaccat tagcaattca acaacttata
     2401 agaaatacgg agaacatccg accccgtttc acatcgtctt ttgacgatat aacgggagaa
     2461 accatgacac tagaccaatt gtttgaaagc ttcatccgcg aaaagcaata catcggaaac
     2521 tgtacgcctc gcacaattac gttctttcga caatctttta aaactttccg ccgaatagta
     2581 accgaggatg aaatgacaag gcaaactacc acagatttcg taattggtgc gcgccaaaag
     2641 ggaatgagtg tcgggtgtct caatgcctat ataaaaggcg ttaactcgtt tctggaatgg
     2701 ctacaccaaa acgaaatcac aaaggaacgg ctgaaaataa aactgctcaa acaagagaaa
     2761 aaggtactgc ggtcttttac agaggatgaa ttgcggcggg tactgagttt caagcccaaa
     2821 acctttggcg aaaggcgcat tttcgcactc acacttacac taattgattc aggcgggcgc
     2881 attgatgaat ttctaagtct gacacgacag caaa
//
LOCUS       DASWNS010000350        20772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_64840, whole genome shotgun sequence.
ACCESSION   DASWNS010000350 DASWNS010000000
VERSION     DASWNS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20772
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64840"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VGB76_12225"
     CDS             <1..319
                     /locus_tag="VGB76_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY0099708.1"
                     /translation="SGLDYYTGLTFKIYVAGAGTRVGGGGRYDNLTGNFGRPEPAVGF
                     VLDLDALTDLLMRRGSSTSDAGSGTDACTLLNSEATEPATLFMDARRRRAGNERVLIN
                     YRG"
     gene            336..977
                     /gene="hisG"
                     /locus_tag="VGB76_12230"
     CDS             336..977
                     /gene="hisG"
                     /locus_tag="VGB76_12230"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY0099709.1"
                     /translation="MPELTIAVAKGRMQDDALQLLSRAGVSVSQAALKSRRLAIEDES
                     GVYRFVFVKPADVPVYVEHGIADCGIVGRDVLLESAADLLQPLDLQIGFCRIAVAAPA
                     RRSQPMIGALRIATKYPQIAAAHFGARGVPVEIIQLSGSVELAPVLGLADCIVDLVET
                     GQTLSENGLEVLEVITESSARLVVNRASYQLKASAVSRLIESLSGGMGKEAAR"
     gene            974..2278
                     /gene="hisD"
                     /locus_tag="VGB76_12235"
     CDS             974..2278
                     /gene="hisD"
                     /locus_tag="VGB76_12235"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY0099710.1"
                     /translation="MIEVIRREDRKGRERKLARIAARSVALDGELMRVVASIVEDVRV
                     RGDEAVVEYTERFDGVRIARDEIRVDERQLSLWAERADGEVRRALGEALKRVRAFHER
                     ERAESWEMETTAGVRLGQRITPVERAGLYVPGGTASYPSSVVMNVVPAQVAGVERIIV
                     ATPPRTLAENPSVAAALLELNVREIYAVGGAQAVAALAYGTQTIPRVDKITGPGNRYV
                     AAAKRLVFGVVGIDSLAGPSEVVVVADASARADFIAADMLAQAEHGADASSVLITTDD
                     ALAFAVASEIERQAQSLARLSIVEKSLADFGAIIVVDNLDEACELVNELAPEHVEIMT
                     REPDAFAARVRHAGAIFLGEHTPEAVGDYFAGPNHVLPTGGAARFSSALGVYDFVKRT
                     SILRYSSIELGRTAQMIATLARAEGLDAHARSALIRLEGVAR"
     gene            2316..3386
                     /gene="hisC"
                     /locus_tag="VGB76_12240"
     CDS             2316..3386
                     /gene="hisC"
                     /locus_tag="VGB76_12240"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY0099711.1"
                     /translation="MADFLDTIKPGVRELRAYTLTPERAAIKLNQNENPWDAPERIKE
                     ESLRRLVGRAWSRYPDFVPVSLHERLARFSGWRADGIIAGNGSNELIQALLMVTIREG
                     KRVLICEPTFALYRQVATVLGGDVLSVPLNAELKYDAAALREKIETSKPDVTIICSPN
                     NPTGCSIGEAELVSLLEVSHGLIAVDEAYFEFSGHTLAGLLEAHPNLVVFRTFSKALA
                     LAALRVGYLLAAPEVAREIGKAVLPYNLNAFSQTVAEVAVELYETELRPLIAQIVAER
                     GRLYEGLQAIDGLAPVRSEANFMVVRSGITPRRVFAELLKRDILVRDVSGYPMLRDYF
                     RVSVGTPRENDLLLAALHEIFA"
     gene            3468..4076
                     /gene="hisB"
                     /locus_tag="VGB76_12245"
     CDS             3468..4076
                     /gene="hisB"
                     /locus_tag="VGB76_12245"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY0099712.1"
                     /translation="MSTQLQTQARTAQVQRRTKETDVRVSLNLDGGGRGRISTGVPFL
                     DHMLELFARHGLFDLEVECRGDLEIDDHHSVEDIAISLGEAFARALGDKRGITRYGAS
                     LVPMDEALCRAVVDLSGRFYLVYEVETRRQAIGNFSVELAEHFWRSFAEALRCNLHLD
                     LLRGRNTHHILEATFKATARALRQAVELDARVEGVPSTKGVL"
     gene            4073..4702
                     /gene="hisH"
                     /locus_tag="VGB76_12250"
     CDS             4073..4702
                     /gene="hisH"
                     /locus_tag="VGB76_12250"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY0099713.1"
                     /translation="MSVRAEVAIIDYGVGNLRSVEKAFGAMNCEAVVSGDERVLRAAK
                     RLVLPGVGAFGACMNALRERGFDELVRERAAEGTPLLGVCVGMQMLFEESEEFGLTEG
                     LGLLGGRVRRFPKELLVPQTGWNQVSQRVAHPLLAGVGDGAFFYFVHSYYCEPERREV
                     VLGETEYGAAYASVVARGAICGVQFHPEKSQAAGLRLLRNFAEFGTVEN"
     gene            4703..5485
                     /gene="hisF"
                     /locus_tag="VGB76_12255"
     CDS             4703..5485
                     /gene="hisF"
                     /locus_tag="VGB76_12255"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY0099714.1"
                     /translation="MLAKRIIPCLDVEGGRVVKGIRFLSLVDAGDPVEQAKRYDAEGA
                     DELTFLDITASADKRAIVSELVRRVADELFIPFTVGGGLGSLEEIRAVLRAGADKVTL
                     NTAAIKHPELISAGAETFGSQCMVVAIDARRRERGKGESGGVVDGWDVFTHGGRRNAR
                     LDAVEWAVRAERLGAGEILLTSMDADGTRNGFDIELTRAVADAVRIPVIASGGAGRLE
                     HFYDALTAGRASAVLAASLFHFGEYKVSEVKNYLRGRGIVVR"
     gene            5482..6159
                     /gene="hisIE"
                     /locus_tag="VGB76_12260"
     CDS             5482..6159
                     /gene="hisIE"
                     /locus_tag="VGB76_12260"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEY0099715.1"
                     /translation="MTKYAPEELRYDERGLLPVVVQDAETREVLTVAYMNAESLRRTL
                     ESGETWFWSRSRAALWHKGETSGHTQRVLGVRVDCDQDALVVLVEPSGAACHTGARSC
                     FQHEIESAGQFDEGDAGGNVGASRALGATLRELYALIESRSRERPAGSYTTYLFAQGL
                     DKILKKVGEEAAETIIAAKNADAHALVGEVSDLLYHLLVLLVERGVKLEEIGCELARR
                     EGRRKAG"
     gene            6156..6941
                     /locus_tag="VGB76_12265"
     CDS             6156..6941
                     /locus_tag="VGB76_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0099716.1"
                     /translation="MNQEQIELEELLEFAVSLARGAGDITLRHFRQPFTPDRKADGSF
                     VTQADREAERFIRSRIEEKFPRDAVLGEEEAARAGSSGRRWIIDPIDGTYAFVHGVPL
                     YGVLIGLEIAEEACLGVVNLPALGEIVYAARGVGCFWNDARARVSATSSLADALLLST
                     DFGTCERYGFGAAARRLEQSAGARRTWGDCYGHVLVATGRAEVMLDPVMNVWDCAPLL
                     PILEEAGGTFTDWNGERTIQGGNAISTNGLLLEAVLGHVRQED"
     gene            6998..7753
                     /gene="hisA"
                     /locus_tag="VGB76_12270"
     CDS             6998..7753
                     /gene="hisA"
                     /locus_tag="VGB76_12270"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY0099717.1"
                     /translation="MLIIPAIDLKNGQCVRLTQGRRAEAKIYDGDPVEIARRFEAAGA
                     RMLHVVDLDGAFAESGSKNREVARRIIEAVAVPVQFGGGLRSLADVRRVIEYGAARVV
                     VGTLVVESAELLAQLVELFGASIVAGIDARDGQVVTRGWEKEARISAVGLAERVATAG
                     VGRIVYTDVARDGMLEGVNVEQTCAVARRANVPVTASGGVSSLAEIARLKVACEGRID
                     SVIIGKAVYEKRFTLAEANEIAGDAEETAARES"
     gene            complement(7901..9880)
                     /locus_tag="VGB76_12275"
     CDS             complement(7901..9880)
                     /locus_tag="VGB76_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099718.1"
                     /translation="MKLKKQQLLKLFTIVLAISLAVGSLLFGAAPGKTSAATYASMQE
                     TTDPAEEATPQAESMEATPLERETSEGNALLLVRFVRDAKVAPTVTTVIEEREVILRD
                     DGLESDQTAGDGTYSAIAFIDFNALADNQERVSQQNANPEPSPVEETTQPTTRTTTGT
                     VATSDARTASADDAELAASSSAETTSDGTERESLASSDSVLMPAFENSRDRQGTIAVP
                     KVDFRNLKPGQFVPIKPLATYYGIDPRKSLVITDVKVVEDPTRTFNPCTNKGTPMGAW
                     TFGRLMTEMANQPLTGIAPPTFVRQWLNKWMANQTTPNGWTAAKRQQIQNLVIGPWEA
                     ASGGPGMPLNLSKAPFRLLAIVNRVDLRSAGTGYGGGNAGEGRFVFGVMDMRPTKGGG
                     IDPYTGLPATACAPTQFTVIIEYGIDRKGCPAIRNWGQQWYNLKWHALGSWSYNAALQ
                     AITDQFTKANVAPGKPNGSALNQLRTNEIAIANLAPDDKWQLREFRLPKGNGHLFEAN
                     AKQTPHETLRNTDLTAHFVNSTPKGATVPDFWLTQKFLTNASDVPSPAVFWSDGPTIT
                     IMPRGDRHDFSLNTCSGCHAGESATIFTHIKPAPFGTPAGLSGFMTGINVADPADGAP
                     TRNFNEFKRRALDLDKLVHTPCFLSMLSAPPPAVH"
     gene            complement(10526..11857)
                     /locus_tag="VGB76_12280"
     CDS             complement(10526..11857)
                     /locus_tag="VGB76_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099719.1"
                     /translation="MSDGQQILREGQTVRTEPSGMACTAEKLLGAGGQGEVYKATLGG
                     EAVALKWFFPQMSTPEQRAAIELLVRKGAPSEHFLWPLELAVEERVEGFGYVMGLRPP
                     RFKNIVDLMKRRIEPTFRALATAGFHLAHNYLLLHTQGFCYRDISFGNAFFDPDTGDV
                     LICDCDNVAPDGRGTLGVLGTPRFMAPEVVLGTAVPSTQTDLFSLAVLIFYMLTVSHP
                     LEGKKETEIKCLDLPAMNKLYGSEPIFIYDPANDANRPVPGIHDNALAFWRIYPQFLR
                     DTFTRAFTEGIRDPLHGRVRESEWRGQMIRLRDSIIYCSHCGLENFYDADRLRATGGN
                     PGLCWSCAVQLVLPPRIRIGSQVVMLNHDTQLYPHHTDDDRLYDFSSPAAAVTRHPSN
                     PNIWGLKNLSGHKWVVTTADGAVRDVEPERSVTLAVKTRIQFGKAEGEIRV"
     gene            complement(11886..13511)
                     /locus_tag="VGB76_12285"
     CDS             complement(11886..13511)
                     /locus_tag="VGB76_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0099720.1"
                     /translation="MNNLPRQKLLEIIRRHGHEIIDAPRRCEGLMRDNFPAHRREIAV
                     LNAALEERIPAELLAPGNSRMPRATLLARLSARLHEDVAMEANAARWTVRTWALALGV
                     ISPDELAALEQVDQRQNEQAARPTTPQPSTRLAQPQTPRASQSQSGAHASPPPANPAA
                     ATRATPAPPSSSAARPSFVVALDGSGDFQTLTDALRRAPSGSLLFVRPGIYNEGLLID
                     KSLEIVGDGALEEIIVRATTSSCLVMRTDAAIICNLTLRGQARAGGAGGDGFFAVDIA
                     HGRPLFEACDISSDSLACVAIHNPTAAPLLRNCHIHHGVDSGVYAFDGAQGLLEACDI
                     AANSNIGVAITSGASMSLRDCLIHHGTDAGVVVWNRATTAIEGCDIYMNRRANVGISD
                     TGVATLRRCRIHDGENTGVFTHREGRATLEACNIYRHAEAEVAVTSGGELSLRGCQIH
                     DGQAHGVFVQDAGHALLETCDLFRNRRSGLHVDAYGVAIARTCRINDNGHVGVSCEAG
                     GAVEVEGCDLTGNLVAAWETDHGSQVVSRNNRL"
     gene            complement(13480..14718)
                     /locus_tag="VGB76_12290"
     CDS             complement(13480..14718)
                     /locus_tag="VGB76_12290"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY0099721.1"
                     /translation="MTDSTETSHAQPADAPPEAKDAKAGAAGATTEASAASKEAGSSA
                     PPNAPTLPDTSMSPDTSAPSAEAASAPRVESPPQAAPVSDALVAERAAAEWRVIGETV
                     PGASHLRAGIPNQDAILHVRQSSARLPVIVSISDGHGSNKCFRSDRGSRFAVRIGADL
                     LDELINGRHAALPPAEMEDRVRQTLPAEFIRRWRAAVEADLKREPFREEEFARMVQKD
                     GANARRLVEANPYLAYGATSLSFLLTPSYALYLQLGDGEMVTVSAQGEIGHPLPEDSR
                     LLANETTSLCLDKAAADFRFAVHPHGAGDLPALILLTTDGYYNSFSTIAGFHQVGSDL
                     LQMLREPDGFGTVNRSVKGWLEEATAAGSGDDCTLAIVCRMDALTASPSSSDTTSTGA
                     PPAVPTPAASHEQSSTPKTA"
     gene            complement(14730..15530)
                     /locus_tag="VGB76_12295"
     CDS             complement(14730..15530)
                     /locus_tag="VGB76_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0099722.1"
                     /translation="MSRRPGGELASRPLHFIWIADASGSMQQDGKIQALNNAIREAIP
                     HMKKVADDNPNAQVLVRAVKFSDGAQWHISQPTPVADFAWNDLPAEGETDMGKALSLV
                     AEQLKMPPMSERALPPVLVLISDGQPTDDFDKGLKDLLDQPWGKKAVRIAISIGQDAD
                     ADVLQKFIAHPEMKPLAANSPEALVKHIKWASTAVLKSASSPAAGGTDHPDALPAAAG
                     SPVGAPAPGANAADPNAAASANAAQPAGAAVPTATDPADDQKTVDDVW"
     gene            complement(15717..16535)
                     /locus_tag="VGB76_12300"
     CDS             complement(15717..16535)
                     /locus_tag="VGB76_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099723.1"
                     /translation="MNDLPRHTLSRIIAEHGRGICDAPKRVEALLRDLCGAHRREINI
                     ISGALEERVAADLIAVGNSVPRDVLLARLAARLRDNLAYTPEAARWGVETWAVALGIL
                     SEAELQERARRESSEAERSNAQPPVEKPKLPTANPPSVNQSTPPMQRGTTYAPSPQQP
                     AQTPKTNPSPQRNPSPQTNRPQPSIARQPAPAPRRTPTVHPHVIVHGSPPQQVSPSLP
                     DQQKPRRGSRLRGCLVLFVLIILFIVGAVLVAPAVIMLLKEEQAQPSINEPRIP"
     gene            complement(16670..17521)
                     /locus_tag="VGB76_12305"
     CDS             complement(16670..17521)
                     /locus_tag="VGB76_12305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEY0099724.1"
                     /translation="MSYAHRLKGKHQIKLRDFAPADDGGLKRKEGEEKTARLIEELVE
                     LQELLYAVRLQSVLVVLQGRDTSGKDGTIRNVAGPLDSQGCTVASFKVPTAEELAHDF
                     LWRVHAQTPPVGHIQIFNRSHYEDVLVARVHKLAPEEVWQKRYEHINAFERLLADSST
                     IILKFYLHISKEEQEQRLLERQEDPTKSWKLSVGDWKEREFWDDYTAAYEDALNRCAA
                     KHAPWFIVPADKKWFRNLAVADALRDALMPHRESWLERLEEMSRAGRREIEEYQRNQQ
                     QQQRRSS"
     gene            17699..17914
                     /locus_tag="VGB76_12310"
     CDS             17699..17914
                     /locus_tag="VGB76_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0099725.1"
                     /translation="MTNDKQKRETLTLTEAQELLGVSRSTLWRILKTYQFQALTDVLD
                     TRVKLVRKVDIERVLADAEKVRQGIAA"
     gene            17911..18726
                     /locus_tag="VGB76_12315"
     CDS             17911..18726
                     /locus_tag="VGB76_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY0099726.1"
                     /translation="MKVISIANQKGGVGKTTVCRELSACCALRGYQTLIIDCDPQGNL
                     TSSWVDAEVYEATLSHVLIEPESTTGIKVEPLPLDDAILESPVENLDLVPADIRLARF
                     EMQPDYLTHRLRNQLQEHGKGYDLVFIDCPPQLGKLLTAALYASDYVIVPCASDAMGL
                     QGLSDLAFTIEQVRKNVNSGLQMLGAVINLYKPQRNLSAESRSVVESAVGLVGHVFDT
                     NLHDYSKVAEAPSQKLPAVLYAKTHRASDQLWSLTEEVLDRLNMSRQKISVVK"
     gene            18735..19790
                     /locus_tag="VGB76_12320"
     CDS             18735..19790
                     /locus_tag="VGB76_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099727.1"
                     /translation="MGETKPKTAGSLREPKPKKTLGLSVPSALRLPHDDLIKPEETPV
                     TPVTPVTPVQTISPVASDAAPKRDFTRVANSVVRDAIPAGLFTGKGKQLYDYLYSQTR
                     GAIVPKRSTRIPTDKVMSGAGMTRHTYRAHLQRLIASGLVQVEEKAGTHGGNMFTVLL
                     PEEIGVERGDRGDRGDRGDRGQDLPLVQGSEVDPCDRGLSLNDSTVYSDSKTSFKTIE
                     KKFDDDAARRFVQSLQEAERELTGKISTDSETWNELAELLVTELKIAAARTTISNVPA
                     FLTEHLRRRLWKVDKGRAHEIAIESGQQNQTALSEEEKRRCPDCAGTNFWYPEGTDKG
                     VAKCRHAKLTQAQTEGK"
     gene            20066..>20772
                     /locus_tag="VGB76_12325"
     CDS             20066..>20772
                     /locus_tag="VGB76_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0099728.1"
                     /translation="MPDENSLLRRFSVQPEVPSASRLIDARVTSAFCERSVSEETRRA
                     YRRVVREFFRFIGARHPEEITPQDVQRWRDVLIAQKKSASTVAFKLSIVRSLFDYLQV
                     GGYVSRNPALAKLVPVPAVSEDLRGRALTAKEVRYLLSGPDREKVAGARDYALLLLML
                     RTSIRVSEACSLRLSQLRWSHGRWIIKFKVKGGRERTQPIPGEVKKAIDEYIKLDRTR
                     RSLQHSDGSEQFIFQPHT"
BASE COUNT         3930 a         5853 c         6887 g         4102 t
ORIGIN      
        1 gtcgggtctt gattattaca cggggctgac tttcaaaatt tacgtggcgg gcgcaggcac
       61 gcgcgtcgga ggcggaggcc gttacgacaa cctgacgggc aatttcggac gccccgaacc
      121 ggcggtcggc ttcgtcctcg acctcgacgc tctgaccgac ctgctcatgc gccggggaag
      181 ttcgacttcg gacgcaggca gtggcacgga tgcctgcacg ttacttaaca gcgaagccac
      241 cgaaccggca acgcttttta tggacgcgcg caggcgacgc gcaggaaacg aacgcgtctt
      301 gatcaactac cggggatgaa agggtgagca ggcagatgcc ggaattaacc atcgcggtgg
      361 cgaaaggaag aatgcaggac gacgcgctgc aactgctgtc gcgcgcgggc gtcagcgtga
      421 gtcaggccgc gctcaagtcg aggcgtctgg cgattgaaga cgagagcggc gtttatcgct
      481 tcgtcttcgt caagcctgcg gacgtgcccg tgtacgtgga gcacggcatc gccgattgcg
      541 gcatcgtcgg gcgcgacgtg ctgctcgaat ccgccgcaga cctcttgcaa ccgctcgacc
      601 tgcaaatcgg attttgccgg atcgctgtgg ccgctcccgc gcgcaggtcg cagccgatga
      661 tcggcgcgct gcgcatcgct accaagtatc cgcagatcgc agccgcacac ttcggcgcgc
      721 gcggcgtgcc cgtcgagatc attcaactgt ccgggtcggt ggaactcgcg cccgtgctcg
      781 gcctcgccga ctgcatcgtc gatctggtgg agacggggca gacgctatcc gagaacggcc
      841 tcgaagtgtt ggaagtcatc acggaatcgt ctgcgcgcct cgtggtcaac cgtgcgagtt
      901 atcaactgaa agcgtctgcc gtctcgcgtc tcatcgagtc gttgagcgga gggatgggaa
      961 aggaagcggc acgatgatcg aagtgattcg cagggaagac cggaaagggc gcgagcgcaa
     1021 actggcgcgc atcgcggcac gcagcgtcgc gctggacggc gaactgatgc gggtcgtggc
     1081 ctctatcgtc gaagacgtgc gggtgcgcgg cgacgaagcc gtcgtcgaat acacggagcg
     1141 tttcgacggc gtgcgaatag cgcgcgatga aataagagtt gacgagcggc agttgagtct
     1201 ctgggcggaa cgcgcagacg gggaggtgcg gcgcgcttta ggcgaagcgc tcaagcgtgt
     1261 gcgcgcgttt catgaacggg agcgcgcaga gtcctgggag atggagacga cggcgggcgt
     1321 tcgtctcggc caacgcatca cgccggttga gcgggcgggt ctgtacgtgc cgggcgggac
     1381 ggcgagttat ccttcgtcgg tcgtgatgaa cgttgttccc gcgcaggtgg cgggcgtcga
     1441 acgcatcatc gtagcgacgc cgccgcgcac gctcgcggag aatccttcgg tagcggctgc
     1501 gcttctcgaa ttgaacgtca gggagattta cgccgtcggc ggcgcgcagg cggtcgccgc
     1561 gctcgcttac ggcacgcaga cgattccgcg cgttgataag atcacgggac ccggaaaccg
     1621 ttatgtcgcg gcggcgaaga gactggtctt cggggtcgtc ggcatagatt cactggctgg
     1681 tccgagcgag gtcgtcgtcg tggccgacgc gtcggcgcgt gccgatttca tcgccgccga
     1741 catgctcgcg caggcggaac acggcgcaga cgcttcctca gttctcatca cgacagacga
     1801 cgcgctcgcc ttcgccgtgg cctccgagat cgagcggcag gcgcagagcc tcgcgcgtct
     1861 ctctattgta gaaaaatctc tcgcagactt cggggcgatc atcgtcgtgg acaacttaga
     1921 cgaagcgtgc gaactcgtta acgaacttgc gccggagcac gtcgagatca tgacgcgtga
     1981 gccggacgcg tttgcggcgc gcgtgcgaca tgcgggagcg atctttttag gtgagcacac
     2041 gccggaagcg gtcggcgatt acttcgctgg tcctaatcac gtcctgccga ccggcggggc
     2101 ggcgcgcttc tcttcggcgt tgggcgtcta tgatttcgtc aaacgcacga gcatcctgcg
     2161 ttactcgtcc attgaactcg gacgcaccgc gcagatgatc gccacgctcg cccgcgcgga
     2221 aggactcgac gcgcacgcgc gttccgcgct catccgtttg gaaggtgtcg cacgttgaga
     2281 cgcgaagacg cgaaccggaa gcagggagac tagcgatggc cgattttttg gacacgatca
     2341 agccgggcgt gcgagagctt cgcgcttaca cgctgacgcc ggagcgcgcc gcgatcaagc
     2401 tcaaccagaa cgagaatccg tgggatgcgc cggagagaat caaggaggag agtttgcgtc
     2461 gcctcgttgg gcgcgcgtgg tcgcgctatc cggatttcgt gcccgtaagt ttgcatgagc
     2521 ggctcgcgcg ctttagcggc tggcgggctg acggcatcat cgcgggcaac ggctcgaacg
     2581 aattgatcca agccctcttg atggtgacca tccgggaagg aaaacgcgtg cttatctgcg
     2641 agccgacgtt tgcgctctac aggcaggtcg caaccgtgct cggcggcgac gtgttgtcgg
     2701 tgccgctcaa tgcggagttg aaatatgacg cggcggctct cagggagaag atcgagacga
     2761 gcaagccgga cgtgacgatc atctgttcgc ccaataaccc gacggggtgc agcatcggcg
     2821 aagcagaact cgtctcgctg ctcgaagtct cgcacggact catcgccgtt gacgaagcct
     2881 atttcgagtt ttcgggacac acgctcgcag gattgttaga ggctcatccg aacctcgtcg
     2941 tctttcgcac cttctcgaag gcgttggcgt tggcggcctt gcgcgtcggc tatttgctgg
     3001 ccgcgccgga ggtggcgcgc gagatcggga aggcagtgct gccgtacaac cttaacgcct
     3061 tctcgcagac agtcgcggaa gtcgcagtcg agttgtacga gacggaactg cgcccgctca
     3121 tcgcacagat cgtcgccgag cgcgggcggc tgtacgaggg tctacaggcg atagacggac
     3181 tcgcccccgt gaggtcggaa gcgaatttca tggtcgtgcg ttccgggatc acgccgcgcc
     3241 gcgtctttgc agaactgctc aagcgcgaca ttcttgtccg cgacgtgagc ggctatccga
     3301 tgctgcgaga ttatttccgc gtgagcgtcg gcacgccgcg cgagaacgat ctgctgctcg
     3361 cggctttgca tgaaattttc gcctgaattt ttttgtgtga agcaattact aacgtccggc
     3421 gtctgccgga gcgcgacttt gcactttcgg atcgtgagag aacatttatg agcactcaac
     3481 ttcagacgca ggcgcgcacg gcgcaggttc aacggcggac gaaagagaca gacgtgcgag
     3541 tttcgttgaa cctcgacggc ggcgggcggg ggcgcatctc gacgggcgtt cccttcctcg
     3601 atcatatgct cgaactcttc gcacgccacg gcctcttcga tctcgaagtg gagtgtcgcg
     3661 gcgatctgga gatcgacgat catcattcgg tggaagacat tgccatctct ctgggcgagg
     3721 ctttcgcacg cgcgcttgga gacaagcgcg gcatcacgcg ttacggcgcg tcgctggtgc
     3781 cgatggacga ggcgttgtgc cgcgcggtgg ttgatctgtc gggtcgcttc tatctcgtct
     3841 atgaagtgga gacgcggcgg caggcgatag gtaatttctc ggtcgagttg gccgaacatt
     3901 tctggcgctc tttcgccgaa gccctgcgct gcaacctgca cctcgacctg ctgcgcgggc
     3961 gcaacactca ccacatcctc gaagcgacgt ttaaagcgac cgctcgcgcg ctacgtcagg
     4021 cggtggaact cgatgcgcgc gtcgagggcg ttccgagcac gaaaggagtt ttgtgagcgt
     4081 gcgcgcggaa gtggcgatta ttgattacgg cgtcggcaat ctgcgctcgg tcgagaaggc
     4141 tttcggggcg atgaattgcg aggcggtcgt gagcggcgac gagcgtgtgc tgcgcgcggc
     4201 gaagcggctg gtgctgccgg gcgtgggcgc gttcggcgcg tgcatgaatg cgctgcgcga
     4261 gcgcggcttc gacgaactcg tgcgcgaacg tgcggcggag ggcacgccgc tgctcggcgt
     4321 gtgcgtcggg atgcagatgt tgtttgaaga gtcggaggag ttcggactga cggaggggct
     4381 ggggctgctc ggcgggcgcg tgcgacgttt cccgaaggag ttattggttc cgcagacggg
     4441 ttggaatcag gtttcgcagc gcgtcgcgca cccgctgctc gcgggcgtcg gcgacggggc
     4501 tttcttctat ttcgtacact cttattactg cgagccggaa cggcgagaag tcgtcttggg
     4561 ggagacggaa tacggcgcgg cttatgcttc ggtcgtcgcg cgcggagcga tctgcggcgt
     4621 ccagtttcac ccggagaaga gtcaggcggc gggcttgcgt ttgctgcgaa actttgcgga
     4681 gtttgggacg gtggaaaact agatgttagc caaacgcatc attccgtgtc tcgacgttga
     4741 aggcgggcgc gtggttaaag gaatacgttt tctgtctctg gttgacgcgg gcgatccggt
     4801 cgaacaggcg aagcgttacg atgccgaagg cgcggacgag ttgaccttcc tcgacatcac
     4861 cgcctctgct gacaagcgcg ccatcgtctc ggaactcgtg cgccgcgtgg ccgatgaact
     4921 gttcatcccg ttcacggtcg gcggcggcct cggcagcctc gaagagatac gcgcagtgct
     4981 tcgcgccggt gcggacaagg tgacgctgaa cacggcggcg atcaaacatc cagaattgat
     5041 cagcgcgggg gcggagactt tcggcagcca gtgcatggtc gtcgccatcg acgcgcggcg
     5101 aagagagcgt ggcaaagggg agagcggcgg cgtggtagac ggttgggatg tcttcacgca
     5161 cggcgggcgg cgaaacgctc ggcttgatgc cgtcgaatgg gctgtgcgcg cggagaggct
     5221 cggagcgggg gagatacttt tgacgagcat ggacgcagac gggacgcgca acgggttcga
     5281 catcgaactg acgcgagccg tggccgatgc ggtgcgcatc ccggttatcg cttcgggcgg
     5341 cgcggggcgg ctcgaacatt tttacgacgc gctcacagcg gggcgtgcga gtgcggtgct
     5401 cgccgcgtcg ctctttcatt tcggagagta caaggtttcc gaggtcaaga attacttgcg
     5461 cgggaggggg atcgtagtgc gatgacgaag tatgcgccgg aagagttgag atacgacgaa
     5521 cgcgggctgc tgccggtggt cgtgcaggat gcggagacgc gcgaagtgct caccgtggct
     5581 tacatgaacg cggagagcct gcggcgcacg ctggagagcg gtgagacgtg gttctggtca
     5641 cgttcgcgcg cggctctctg gcacaagggc gaaacttccg gccacacgca acgcgtcctc
     5701 ggcgtcagag ttgattgcga tcaggatgcg ctcgtcgtgc tcgtcgagcc gtcgggtgct
     5761 gcttgtcata cgggcgcgcg ttcatgtttt caacacgaga tcgagagtgc gggtcaattt
     5821 gatgaaggcg acgctggcgg gaatgtcgga gcgtctcgag cgctcggcgc gacgctcagg
     5881 gagctttacg cgctcatcga gagcaggagc cgggagcgac ccgcagggtc ttacacgacc
     5941 tatctgtttg cgcagggatt ggataagatt ttgaaaaagg tcggggaaga agcggcggag
     6001 acgatcatcg cggcgaagaa cgcagacgcg cacgcgctcg tcggcgaagt ctccgacctg
     6061 ctctatcacc tgctcgtgct gctggtcgag cgcggcgtta agttggaaga gatcgggtgc
     6121 gaactggcga gacgcgaggg gagacgaaaa gccggatgaa tcaggaacag attgaactcg
     6181 aagagttgct tgaattcgcc gtctcgctcg cgcgcggggc gggcgacatc acgctgcgcc
     6241 acttccgcca gccgttcacg cccgacagga aggcggacgg ctcgtttgtg acacaggcgg
     6301 acagagaagc cgaaagattc atacgctcgc gcatcgaaga gaaatttccg cgcgacgccg
     6361 tgctcggaga agaggaagcc gcgcgcgcgg gttcgtccgg caggcgatgg atcatcgatc
     6421 cgattgacgg aacttacgcg ttcgttcatg gcgtgccgct ctacggcgtt ctgataggtc
     6481 tggagatcgc ggaggaggct tgtctgggcg tcgtcaattt accggcgctt ggcgagatcg
     6541 tctatgcggc gcgcggcgtg ggctgcttct ggaacgatgc gcgggcacgc gtctccgcca
     6601 cttcatcgct cgccgatgcc ctgctgcttt cgactgactt cgggacgtgt gagcgatacg
     6661 gcttcggcgc ggcggcgcgc aggttggagc agagcgcggg cgcgcgtcgc acgtggggcg
     6721 attgctacgg ccacgtcctc gtcgccaccg gacgcgccga ggtgatgctc gaccccgtga
     6781 tgaacgtgtg ggattgcgcc ccgttgctgc cgatactcga agaagcgggc gggactttta
     6841 cagactggaa cggcgagcgc acgattcaag gcggcaacgc catctccacc aacggcctgc
     6901 tgctcgaagc cgtgctcgga cacgtcaggc aggaagacta gccgcgccgt cgctcccgcg
     6961 cttcgattca ttccatgaga gggacttgag aattcagatg ttgattattc cggcgattga
     7021 tctaaagaac ggccagtgcg tgcgtctgac gcagggacga cgagcggagg ccaaaatcta
     7081 cgacggcgac ccggtcgaga tcgcgcgaag gtttgaagcg gcgggcgcgc ggatgctgca
     7141 cgtcgtagac ctcgacgggg cgttcgcgga gagcggctcg aagaaccggg aggtggcgcg
     7201 gcgcatcatc gaagccgtcg ccgtgccggt tcagttcggc ggcggcctgc gtagccttgc
     7261 ggacgtgcga cgtgtgatcg aatacggtgc ggcgcgggtg gtcgtcggca cgctggtcgt
     7321 ggagtctgcg gaactgctgg cgcaactcgt cgagctattc ggcgcgagca tcgtcgcagg
     7381 gattgacgcg cgtgacgggc aagtcgtcac gagaggttgg gagaaggagg cgcgtatctc
     7441 cgccgtcgga cttgcggaac gcgtggcgac ggcaggcgtc gggaggatcg tctatacaga
     7501 cgtggcgcgc gacggcatgc ttgagggcgt gaacgtagaa cagacgtgcg ctgtcgcgcg
     7561 ccgtgcgaat gtgcccgtca cggcatcggg cggcgtctct tcgctggcgg agatagcgcg
     7621 tctgaaagtt gcgtgcgaag gcaggattga tagtgtgatt atcggcaaag ccgtttatga
     7681 aaaacgcttc acgctggccg aggccaacga gatcgcaggc gacgcggaag agacggcagc
     7741 gcgcgagagt tagcctgtga aagaccctga aaaagagaga ggccggaaga cagattgagc
     7801 attgtcttcc ggcctctctc atcaatgacg cgttcggcgt cgaagcttct ctaccatgat
     7861 cgccggcgcg ttgcgagcga tgcgtcgcct cacgtatgaa ttagtggaca gccggaggcg
     7921 gagcactaag catactgagg aagcagggcg tgtggacgag tttgtcgagg tcgagcgcgc
     7981 gacgtttgaa ctcgttgaag tttcgcgtcg gcgcgccgtc tgccggatcg gccacgttga
     8041 tgccggtcat aaagccggac agcccggcgg gcgtcccgaa gggcgcgggc ttgatgtgcg
     8101 tgaagatggt ggcagactct cccgcatggc acccgctaca ggtattgagg gagaagtcat
     8161 gtctatcgcc gcggggcatg atggtgatgg ttggaccatc gctccagaag actgcgggcg
     8221 aaggcacgtc ggacgcgttg gtgaggaact tctgggtgag ccagaagtcc ggcacggtcg
     8281 cccccttcgg cgtgctgttg acgaagtgtg cggtcaggtc tgtgttcctg agcgtctcgt
     8341 gcggcgtctg tttggcattt gcctcgaaca ggtggccgtt ccctttcggc aggcgaaact
     8401 cgcgcagttg ccacttgtcg tcgggtgcaa gattggcgat ggcgatctcg ttggtgcgaa
     8461 gttggttgag cgcgctgccg ttgggcttgc cgggcgcgac gttggctttg gtaaactggt
     8521 ctgtgatggc ttgcagcgca gcgttgtaag accatgaacc gagcgcgtgc cacttcaggt
     8581 tgtaccactg ctgaccccag ttgcgaatgg cggggcagcc cttccggtcg atgccgtatt
     8641 cgatgatcac tgtgaactgc gtcggcgcgc aggccgtcgc cgggaggccg gtgtaggggt
     8701 cgatgcctcc tcctttggtc ggcctcatgt ccatgacgcc gaagacgaag cgaccttcgc
     8761 ccgcgttgcc gccgccgtag cctgtgcccg cgctgcgcag gtcaacgcgg ttgacgatgg
     8821 cgagcaggcg gaaaggagct ttcgacagat tgagcggcat gcccggacca cccgatgcgg
     8881 cttcccacgg accaattacg aggttctgta tctgctggcg tttggccgcc gtccagccgt
     8941 tgggcgtggt ctggttagcc atccacttgt tgagccactg gcgcacgaag gtaggcggcg
     9001 cgatgcccgt gagcggctgg tttgccatct ccgtcatcag gcgtccgaac gtccacgcgc
     9061 ccatcggcgt gcctttgttc gtgcaggggt tgaaggtgcg tgtcgggtct tcgacgactt
     9121 tcacgtcggt aatgacgagc gactttctag gatcgatgcc gtagtaggtg gcgagcggtt
     9181 tgatcggcac aaactggccg ggcttcaagt tgcggaaatc cactttcggc accgctattg
     9241 tgccctgccg gtcgcggctg ttttcaaacg cgggcatgag cacggaatcg gaagaggcga
     9301 gcgattccct ttccgtcccg tcagaggtgg tttcggcgga agaggaggcg gccaactcgg
     9361 cgtcgtccgc gctggccgtc ctggcgtcgc tggttgcgac ggttcccgtc gtcgtgcggg
     9421 tggtcggctg cgtagtctct tccaccggac tcggctccgg attggcgttt tgttgagaga
     9481 cgcgctcctg attgtcggcc agcgcgttga aatcgatgaa ggcgatggcg gagtaggtgc
     9541 cgtcgcccgc cgtctggtcg ctttcgagtc cgtcgtcgcg gaggataact tcgcgttctt
     9601 cgatcaccgt cgtgacggtc ggcgcgactt tcgcgtcgcg cacgaaccgc acgagcagga
     9661 gcgcgttgcc ctcggatgtc tcgcgttcga gcggcgtcgc ctccatggat tcggcttgcg
     9721 gcgtggcctc ctcagccggg tcggtcgtct cctgcatgct tgcgtaggta gcggcgctcg
     9781 tcttacccgg cgcggctccg aagagcaggc tgccgacggc cagagagatt gccagtacga
     9841 tggtgaagag tttgaggagt tgttgcttct tgagtttcat gggtctcctt ctacgttgtg
     9901 agcacaagtt gtgagtaaaa ctttttgttg tgagagtgaa acgagacttc gctttcgagg
     9961 ggcgctggcg aagactcgtc ccgttgacga cgcggaaacc tgcgttgagc agcccggaat
    10021 caaaactgct gcgcgcaagc gaccgcgata gatgcaaagc cttgaagaaa agaagagaga
    10081 atggcgacga gacgaatttt caaagagcag ccgacggcgc ggaataaggc acaccgtcac
    10141 cttctgccgg gcggtagtta catcagcaag cgttcaaatt aggtaaaact gtccggcggt
    10201 tgcaggaatg caatccgtgg gggcggatac gccgtctgtt aatttcggga gtgaggctta
    10261 ggggcgcagt ctcaacttgt cagtactgac tgataattca gccctcaaaa ttcaagacat
    10321 ccgaacggca tcaagacgca gctcgcacat caaaatttac agctctcgcc aaaagtttga
    10381 ggttggggat ttatgtgcag ggaggatgat acaccctttc gatgaggtgt caatattaaa
    10441 ttttactgac gcgcgaaccg gcgcgttacc ggcgacgcgt ttagtaaaat attgcccaat
    10501 cagtagagac tacaggccct ctcaatcaca cgcgaatttc gccttccgcc ttgccgaact
    10561 ggatgcgggt tttgacggcg agcgtcacgc tgcgctccgg ttcgacatcg cgcaccgcgc
    10621 cgtcggcagt tgtcacaacc cacttgtggc ctgagagatt tttcaatccc cagatgttag
    10681 ggtttgacgg gtggcgcgtg acggcggcgg cggggcttga gaagtcgtag aggcggtcgt
    10741 cgtccgtgtg atgcgggtag agttgcgtgt cgtggttgag catcacgact tgactgccga
    10801 tgcgtatgcg cggcggaagc acgagttgca cggcgcacga ccagcagagg ccggggttgc
    10861 cgcccgttgc gcgcaggcgg tctgcgtcgt agaagttttc cagcccgcag tgtgagcaat
    10921 agatgatcga atcgcgcagg cggatcatct gcccgcgcca ttcgctttcg cggacgcgcc
    10981 cgtgcagcgg atcgcgtatc ccttcggtga aggcgcgcgt gaaggtgtcg cgcaggaatt
    11041 gcgggtagat gcgccagaag gcgagcgcgt tatcgtgtat gccggggaca ggccggttcg
    11101 catcgttcgc ggggtcgtag atgaagatgg gttcgctgcc gtagagcttg ttcatggcgg
    11161 gcaggtcgag gcatttaatc tccgtctcct tcttgccttc gagcgggtgc gagacggtga
    11221 gcatgtagaa gatgagaacg gcgagcgaga agagatcggt ctgcgtactc ggcacagccg
    11281 tgccgagtac gacttcggga gccatgaagc gcggcgtgcc gaggacgccg agcgtgccac
    11341 ggccatcggg cgcgacgttg tcgcaatcgc agatcagcac gtcgcccgtg tcagggtcga
    11401 agaaggcgtt gccgaaagag atgtcgcggt agcagaagcc ctgcgtgtgg agcagcagat
    11461 agttgtgcgc gaggtggaac ccggcggtgg cgagcgcgcg aaacgtcggc tcgatgcgac
    11521 gcttcatgag atcgacgatg ttcttgaagc gcggcggacg caagcccatc acgtagccga
    11581 aaccttcgac gcgctcttcg accgcgagtt cgagcggcca caaaaaatgc tcgctcggcg
    11641 cgccctttcg cacgagcagt tcaatcgctg cgcgctgttc gggcgtagac atctgcggga
    11701 agaaccactt gagcgcgacc gcttcgccgc ccaacgtggc tttataaact tcgccctgcc
    11761 cgcctgcgcc gagcagtttc tccgccgtac acgccatgcc cgaaggctcc gtgcgcaccg
    11821 tctgaccttc gcgtaaaatt tgttgaccgt ctgacataaa cttcccttcc cgctctcgaa
    11881 cgatttcaca aacgattgtt gcgactcacg acctgcgagc cgtggtccgt ctcccacgcg
    11941 gcgacgaggt tccccgtcaa gtcgcagcct tcaacctcga ccgcgccgcc cgcttcacaa
    12001 ctgacgccga catgaccgtt atcgttgatg cgacacgtgc gcgcgatggc gacgccgtaa
    12061 gcatcgacgt ggaggccaga cctgcgattg cgaaaaagat cgcacgtctc caacagcgcg
    12121 tgccccgcgt cctgcacgaa gacgccgtgc gcttgcccgt cgtgaatctg acatccgcgc
    12181 aggctcaact ctccgccgct cgtcacggcg acctccgctt ccgcatggcg gtagatgttg
    12241 cacgcttcga gcgtcgcacg cccttcgcga tgcgtgaaga cgcccgtgtt ttcgccgtcg
    12301 tgaatgcgac agcggcgcaa ggtggcgacg ccggtatcgc tgatgccgac gttcgcgcgc
    12361 cggttcatgt aaatgtcgca accctcgatg gccgtcgtcg cgcgattcca gacaacgact
    12421 ccggcgtccg tgccgtgatg tatgagacag tcgcgcaggc tcatgctcgc gccgctcgtg
    12481 atggcgacgc cgatgttgga gttggcggcg atgtcgcacg cttcgagcaa gccctgcgcg
    12541 ccgtcgaagg cgtagacgcc cgaatctacg ccgtgatgaa tgtggcaatt gcgaagcagc
    12601 ggcgcggcgg tcgggttgtg aatggcgacg caggccagcg agtcggagct gatgtcgcaa
    12661 gcctcgaaca gaggccgccc gtgcgctatg tccacggcga aaaagccgtc gccgcccgca
    12721 ccgcccgcgc gtgcctgccc gcgcaaagtc agattgcaaa tgatcgccgc atccgttcgc
    12781 atgacgagac aacttgaggt ggtcgcacgc acgatgatct cttcgagcgc gccgtcgccc
    12841 acgatctcaa gcgatttgtc tatcaacaga ccttcgttgt aaatgcccgg acgcacgaac
    12901 agtagcgaac ccgaaggagc gcgccggagc gcgtccgtga gcgtctgaaa atcgccgctg
    12961 ccgtcgagcg cgacgacgaa cgaggggcgc gcagcggacg aagaaggagg agcgggagtt
    13021 gcgcgagtcg ctgccgcagg attcgcggga ggcggtgacg cgtgcgcgcc ggactgagat
    13081 tgcgaggcgc gcggcgtctg cggctgtgcc agtcgcgttg atggttgagg tgttgtcggg
    13141 cgcgcggctt gttcgttctg ccgttggtca acctgttcga gggcggcgag ttcgtcgggt
    13201 gaaatgacgc cgagggcgag cgcccaggtg cgaaccgtcc agcgcgcggc gttcgcttcc
    13261 atcgcgacat cttcgtggag gcgcgcggag aggcgtgcga gcagcgtggc gcgcggcata
    13321 cggctgttgc cgggggcgag cagttcggcg gggatgcgtt cttcgagcgc ggcgttgagc
    13381 acggcaatct cgcggcgatg cgcggggaag ttgtcgcgca tcaagccttc gcaacgccgt
    13441 ggtgcgtcga tgatctcgtg accgtggcgg cggatgatct caagcagttt ttggcgtgga
    13501 agattgttca tgcgaagcgg cgggcgttgg cacggcgggc ggcgcgccgg ttgaggtcgt
    13561 gtcgcttgat gaaggcgacg ctgtgagagc gtccatgcgg cagacgatgg cgagcgtgca
    13621 atcgtcgccg ctgcccgcgg cggtcgcttc ttcgagccag cctttgacgc tgcggttgac
    13681 cgtgccgaag ccgtcaggct cgcgcagcat ttgcagcaag tcgctgccga cttgatgaaa
    13741 gcccgcgatg gtggagaaag agttgtagta accgtcggtc gtcaacagga tgagcgcggg
    13801 taggtcacct gcaccgtgcg ggtggacggc gaaacgaaag tcagccgcag ccttatccag
    13861 acagagcgac gtggtttcgt tggcgagcag gcgcgagtct tcgggcaagg ggtggccgat
    13921 ctcgccctgt gcggagacgg tgaccatctc gccgtcgccg agttggagat agagcgcgta
    13981 ggacggcgtg agtagaaaag agagcgaggt tgcgccgtag gcgagatacg ggttggcttc
    14041 gacgaggcgg cgtgcgtttg cgccgtcctt ctggaccatg cgcgcgaact cctcttccct
    14101 gaacggctcg cgcttcaggt cggcctcgac cgccgcgcgc caacgcctga tgaactcggc
    14161 ggggagagtt tggcgcacgc ggtcttccat ctcggcgggc gggagcgcgg cgtgcctgcc
    14221 gttgatgagt tcgtcgagca ggtctgcgcc gatacggacg gcgaagcgcg agccgcggtc
    14281 gctgcggaaa cacttgttgc tgccgtggcc gtcggagatg gagacgatga cgggcagccg
    14341 cgcgctcgac tggcggacgt gcaggatcgc gtcctgattc gggatgcccg cgcgcaggtg
    14401 cgacgcgccg gggacagtct caccgatcac acgccactcg gcagcggcgc gctccgcgac
    14461 gagcgcatcg ctgacgggcg cggcttgggg cggcgactcg acacgcggcg cactcgcggc
    14521 ttccgccgac ggcgcggaag tgtctggcga catggaggtg tctggcagcg tgggggcgtt
    14581 tggcggcgcg gaactgcccg cctcctttga ggccgcgctc gcctctgttg tcgcgcccgc
    14641 tgcgcccgcc tttgcgtcct tagcttcggg cggcgcgtcg gcaggttgcg cgtgcgaggt
    14701 ttccgtcgag tcggtcatgc gacgatcctt taccaaacgt cgtcaactgt tttttgatcg
    14761 tccgcaggat cggtcgcggt cgggacggct gcgcctgcgg gctgcgcggc gttcgccgac
    14821 gcggcggcgt tcgggtctgc ggcattcgcg ccgggcgcgg gtgcgccgac gggcgagcct
    14881 gcggcggcgg gcagcgcatc cgggtgatcc gtgccgcctg cggctggcga agaggccgac
    14941 ttcaacacgg cggtcgaagc ccacttgata tgtttgacga gagcttcggg cgaattcgcg
    15001 gcgagcggtt tcatctcggg gtgcgcgatg aatttttgca gcacgtcggc gtcggcatcc
    15061 tgcccgatgg agatggcgat gcggacggcc ttcttgcccc agggttgatc gagcaggtct
    15121 ttcaagcctt tgtcgaaatc gtcggtgggc tgaccgtcgg agatgaggac gaggacgggc
    15181 ggcagcgcgc gctcggacat gggcggcatc ttgagttgct ctgcgacgag tgacagcgcc
    15241 ttgcccatat cggtttcacc ttccgccgga agatcgttcc aggcgaaatc ggccacgggg
    15301 gtcggctgcg agatgtgcca ctgcgcgcca tcggagaatt tgacggcgcg gacgagcacc
    15361 tgcgcgttcg gattatcgtc cgccaccttt ttcatgtgcg ggatggcttc acgaatcgcg
    15421 ttgttcaaag cctgaatttt tccgtcctgc tgcatcgagc cggatgcgtc tgcgatccag
    15481 atgaaatgaa gtggacgcga agcgagttcg ccgcccggtc gtctgctcat aatcgaatgt
    15541 cctccccgtg aacgagtcag tctgttaatc aaattgttgt taggaactgg aaggaaagat
    15601 acaccatcga gcggagatgc cgaaagcgtg cgggcacgga aatcgaaacg ggaagcaagg
    15661 gtcaggagag aaacgcgcgt gagccgtctg cgcttgtcgt cgtgcgcggg gagagatcaa
    15721 gggatgcgcg gctcgttgat gctcggctgc gcctgttcct ctttcaaaag catgatgacc
    15781 gccggagcga ccaagaccgc gccgacgata aagaggatga tcaagacaaa gaggacgaga
    15841 cagccgcgca gtctcgaacc gcgccgaggc ttctgttgat cgggaagcga cggcgaaacc
    15901 tgctgcggtg gcgacccgtg cacaattacg tgcgggtgaa cggttggcgt gcggcgcggc
    15961 gcgggcgcag gctggcgggc aattgacggc tgaggccggt tcgtctgcgg cgacggattc
    16021 ctctgcggcg acgggttcgt ctttggcgtc tgcgccggtt gctgcggcga aggtgcgtag
    16081 gtcgtgccac gttgcatcgg aggcgtcgac tggttgacgc tcggcgggtt agccgtcggc
    16141 aatttcggtt tttcaacggg cggctgcgcg ttacttcgct cggcctccga cgattccctt
    16201 cgagcgcgtt cctgtagttc cgcttccgac aagattccga gcgcgaccgc ccatgtctcg
    16261 actccccagc gcgcggcttc cggagtgtat gcgaggttgt cgcgcagtcg cgctgcgagc
    16321 cttgccagca acacgtcgcg cggcacggag ttgcccaccg cgatcaaatc cgccgcgacg
    16381 cgttcttcga gcgcgccgct gatgatgttg atctcgcgcc ggtgcgcgcc gcatagatcg
    16441 cgcagcaacg cttcgactcg tttcggcgcg tcgcaaattc cgcgaccgtg ttcggcgatg
    16501 atgcgcgaga gcgtgtggcg tggcaggtcg ttcattgatg caggctcaag ggtcttggat
    16561 tatagaacaa ctcctgcggc ctttcaccac agcgagacgg cgagacgctg aaacagccac
    16621 gccggaaacg acacggctgt cccgaaccga atatgcagcg taaagatcgt tacgatgagc
    16681 ggcgttgctg ctgctgctgg ttccgttgat actcttcaat ctccctgcgc cctgcgcggc
    16741 tcatctcttc cagcctctcc aaccaactct ccctgtgcgg catcaacgca tcccgcaaag
    16801 cgtccgccac cgccagattg cgaaaccatt tcttgtcggc gggcactatg aaccagggcg
    16861 cgtgtttcgc ggcgcaacga ttgagcgcat cctcgtaggc ggcagtgtag tcgtcccaga
    16921 actcgcgctc tttccagtcg ccgaccgaga gcttccatga cttagtcggg tcttcctgac
    16981 gctccagaag tctctgttcc tgctcctctt tgctgatgtg cagatagaac ttgagaatga
    17041 tggtggaaga atccgccagt agacgctcga aagcgttgat gtgctcataa cgcttctgcc
    17101 acacctcctc cggcgcgagt ttgtggactc gcgccaccag cacatcctca taatgcgaac
    17161 gattgaagat ttggatatgt cccacggggg gcgtctgcgc gtgcacgcgc cagaggaaat
    17221 catgcgcgag ttcttccgcc gtcggaactt tgaaggaagc tacggtgcag ccctgagaat
    17281 ccaacggacc cgccacgttg cgaatcgtgc cgtctttgcc gctggtgtcg cgcccttgca
    17341 ggaccaccag cacgctctgt agtcgcaccg cataaagaag ctcttgcagt tcgaccaatt
    17401 cttcgatgag ccgtgctgtc ttctcctcgc cctccttacg ttttaagccc ccgtcgtctg
    17461 ccggagcaaa gtccctcagc ttgatctgat gcttgccctt aaggcgatgc gcatagctca
    17521 taatgttctc ctcaagtaat gccgcgaaaa tatatcaaat cccaacaacc cgataaccac
    17581 ctacgatgac aacacgacgt tatgacgttg acgcgtttga aacatatctg ttgacatctt
    17641 agtgtatcag aggtaaaaga cgctttctta tcatatctag ttctcatgag gtgaactgat
    17701 gacaaacgac aaacagaaac gggaaacact gactcttacc gaagcgcaag aattgctcgg
    17761 cgtctctcgc tccacgctct ggcgcatcct caagacctat caatttcaag cactgacaga
    17821 cgtactcgac acgcgtgtga aactggtgcg aaaagttgat attgaacgcg tgttggcgga
    17881 cgctgaaaaa gtacgtcaag gaatcgcagc atgaaagtaa tctccatcgc gaatcaaaaa
    17941 ggcggggtcg ggaaaactac tgtctgccgt gagctttccg cgtgctgcgc gctcaggggc
    18001 taccagacat tgatcatcga ttgcgacccg cagggaaact taacgagtag ctgggttgat
    18061 gcagaagtct acgaggccac gctctcgcac gtattgattg agccggagtc cacgacgggc
    18121 attaaggtcg agccgcttcc cttagacgat gcgattctcg aaagcccggt cgaaaatctc
    18181 gacctcgttc ccgccgacat tcgactcgcc cgtttcgaga tgcagcccga ttatttgacg
    18241 catcggctga gaaaccaact acaagagcac ggcaagggct acgacctcgt cttcatagac
    18301 tgccccccgc aactcggcaa acttttaacc gcagccctgt atgccagcga ttacgtgata
    18361 gttccctgtg cgtcggacgc gatgggattg caggggcttt ccgatctcgc tttcaccatc
    18421 gaacaggtca gaaaaaatgt caattccggc ctgcaaatgc tgggggcggt gatcaatctc
    18481 tacaagccgc aacgcaacct gtctgccgaa tctcgtagcg tcgtagagtc agccgtaggg
    18541 ctggtgggac acgttttcga tacgaacctc cacgattatt ccaaagtcgc ggaagcgcct
    18601 tcacagaagt tacctgcggt gctgtacgcc aagacgcatc gtgcttccga ccagctctgg
    18661 agtttgacgg aagaagtgct ggatcggctg aatatgtccc ggcagaagat ttccgtcgtc
    18721 aaatgaggtg attaatggga gagactaaac ctaaaaccgc cggttcccta cgcgagccta
    18781 aacccaagaa gactctaggg ttaagcgtgc caagtgccct caggctgccg cacgatgatt
    18841 taatcaaacc ggaagaaacc cctgtcaccc ctgtcacccc tgtcaccccc gtccagacca
    18901 tatcgccggt tgcgagtgat gccgctccga aacgcgactt tacacgcgtg gctaattccg
    18961 tagtgagaga cgcgattccc gccgggcttt tcacaggcaa aggaaagcag ctttacgatt
    19021 acctctattc gcagacacgc ggagccatcg tgcccaagcg ttcgacgcgc attcccaccg
    19081 acaaagtaat gagcggggcg ggcatgacac gccacactta cagggcgcat ctccagcggc
    19141 ttatcgcttc aggtctggtg caagtcgaag aaaaagcggg gacgcatggc ggcaacatgt
    19201 tcacggtttt actcccggaa gagatcggcg tcgagagggg tgacaggggt gacaggggtg
    19261 acaggggtga cagggggcaa gatctacccc tggtacaggg gtcagaagtt gacccctgtg
    19321 acaggggttt atctctaaat gattcaaccg tttacagcga ctctaagact tcttttaaga
    19381 ctatagagaa aaaatttgat gatgacgctg cgcgccgctt cgtccagtcc ctgcaagagg
    19441 ccgagcgaga actcacaggc aagatttcta cagactccga aacctggaat gaactggccg
    19501 aacttctcgt taccgaactg aagatcgctg cggcacgcac caccatctca aacgtcccgg
    19561 cttttctgac cgagcacctt cgccgcaggc tttggaaagt agacaaaggg cgcgcacacg
    19621 aaatcgccat cgagtcaggg cagcaaaatc agacggcttt aagcgaagaa gagaagcggc
    19681 gctgcccgga ctgtgcaggc actaacttct ggtatccaga agggacagac aaaggcgtag
    19741 ccaagtgcag acacgcgaag ctcacacagg ctcagacgga agggaagtga gtctcatccg
    19801 gatgttgcgc gatatgcttc gttatcgtcc gactcggcaa gagagacgag taagaaagag
    19861 agccgcgttt cacgtcgttt cacagtgtct aaataattga atacgattga ctaaatatcg
    19921 agttaatcga gattccgtta cgtggaaaca atatttaagg taatcagtgt attgttaaca
    19981 ttatgcttga cgcactctct acaggaggcg caagattcgt gttgtttagc tggataaagt
    20041 ccgagagggg aggtagcctt tcacgatgcc tgacgaaaat agcctgttaa gaaggttctc
    20101 cgtgcagcct gaagtgcctt cagcgtcccg gctgattgat gctcgtgtca cctctgcctt
    20161 ttgtgaacgc tcggtttcgg aagagacacg ccgcgcttat cgccgggtag tgcgcgaatt
    20221 ctttcgcttc attggagcgc gacatcctga agagattaca ccccaggatg tgcagagatg
    20281 gcgcgacgta ttgatcgcac agaagaagag cgccagcacg gtcgccttca aactctctat
    20341 cgtgcgctcg ctcttcgatt acttgcaggt cggaggctac gtgagccgca accctgcgct
    20401 ggcaaagttg gtgcctgtgc ctgctgtgtc ggaagactta cggggcaggg cactgacggc
    20461 caaagaggtg cgctatttgc tgagcggccc cgaccgtgaa aaggttgccg gtgcgagaga
    20521 ctatgccttg ctgctgctga tgctgcggac ttcgattcgt gtgtcggaag cctgttcgct
    20581 gcgcctgtcg cagcttcgtt ggagtcacgg gcgttggatt atcaagttca aggttaaagg
    20641 gggcagggaa cgcacacagc ccattcccgg cgaggtgaag aaggcgattg atgagtacat
    20701 caagctggac cgcacgcgca gaagtctaca gcattctgac ggctccgagc agttcatctt
    20761 ccagccgcat ac
//
LOCUS       DASWNS010000351         8644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_14379, whole genome shotgun sequence.
ACCESSION   DASWNS010000351 DASWNS010000000
VERSION     DASWNS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8644
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14379"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VGB76_12330"
     CDS             <1..634
                     /locus_tag="VGB76_12330"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY0099729.1"
                     /translation="FFMAHNSSGGTRLSHLDTYATDPVGLHVIIDTPQGSRNKFEYDD
                     ELGLFKLGGVLPAGAVFPFNFGFVPSTKGGDGDPLDVLVLMEEAAFAGCLVVARLIGV
                     IEAKQTEEDGETVRNDRLIAVAEKARNHKEVRSLGQLNEHLLDEIEHFFVSYNEMKGK
                     EFKPLGRFGTERAERLIEEGARRFRAEERKQRPRAQKSRKSSGGAAKTRR"
     gene            complement(868..1077)
                     /gene="rpsU"
                     /locus_tag="VGB76_12335"
     CDS             complement(868..1077)
                     /gene="rpsU"
                     /locus_tag="VGB76_12335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY0099730.1"
                     /translation="MATVNVNQGESIESALRRFKRKVMTEEIIKDAKKHAFFMSKGQK
                     AKLKSALARKRNKKKRRPPQQQPRG"
     gene            complement(1201..1557)
                     /locus_tag="VGB76_12340"
     CDS             complement(1201..1557)
                     /locus_tag="VGB76_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099731.1"
                     /translation="MDKQTYAVGDRVEKWCEACGEDRGHVVATVTKRGLISRVNCPKC
                     GTRSSFKNNAQATGTRSGAQTGTPYDWTRTYRAGQTMLHPTYGLGEVMALVEPKKIDV
                     LFSDRMRRLVHGRADN"
     gene            complement(1643..1864)
                     /gene="infA"
                     /locus_tag="VGB76_12345"
     CDS             complement(1643..1864)
                     /gene="infA"
                     /locus_tag="VGB76_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY0099732.1"
                     /translation="MAKDDLIPAEGVVVDKLPNAFFRVRLDNSEHIVLAQIGGKMRKN
                     FIRILPGDRVQVEMSPYDLSKGRITYRYK"
     gene            complement(2121..2519)
                     /locus_tag="VGB76_12350"
     CDS             complement(2121..2519)
                     /locus_tag="VGB76_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY0099733.1"
                     /translation="MSMKLYVGNLNFKTSGEDLQELFGQVGTVESASVVMDRDTGRSR
                     GFGFVEMSSSEEGQKAIEQFNGKEFNGRNLSVNEARPREDRGGGGGGGGRGGYGGGGG
                     GNRGGGGGYGGNRGGGGGYGGGGGDREPRW"
     gene            3252..5141
                     /locus_tag="VGB76_12355"
     CDS             3252..5141
                     /locus_tag="VGB76_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEY0099734.1"
                     /translation="MKFKTLVLAVSTFFMLVAIPAAAQGQTWQYTALGDSLGAGLYDS
                     QGGYVPRFRNYVQADTGVNVILNNRSVSGWKATDLLHALRTDESLRTQIANSQIVTWN
                     IGANDFQDALLRYQNGTCGGADNQNCIRAAVAKLKVDWNAIVAQILSLRSPSNTILRT
                     MDNYNPAVDLARAFGVLHVIKPYLEEVNRFIFLSAINNRIGCARVYQAFNGVNGEADA
                     GRRGYMSSDGIHPNELGHDIIASEFRALSYAPLVAPQSTISLLLPVTIVNEGDGRATV
                     TVTRGGNTSGAASVEYATLDNPADVPCAAPPSSDDSVSIAFARCDYATTVDALHFAAG
                     ETQKSLNIPLINDAHVENTEEFSIRLTGASGASLGVRTSAVVRIMDNEARVTPPLVFP
                     NPIHTSEFFVRMQYLDFLSREAEPAGFAAWMNVLNNCSDLHLNPACDRTTVTASFFRS
                     EEFNLKAYFVYLFYKTTLGRLPRYTEIIPDMRRVSGETAEEVFARRLAFAYAWVERVE
                     FKSLYPETLSQEEFVDRLLQATGVGLSGSVSRNSLVSDLNTRNKTRAEVLIALVEHPA
                     VDLRERNGAFVAMQYFGYLRRDPDTGGYNNWLNYLNANPNDFRTMVRGFANSQEYQLR
                     FGQAQ"
     gene            complement(5258..6922)
                     /locus_tag="VGB76_12360"
     CDS             complement(5258..6922)
                     /locus_tag="VGB76_12360"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY0099735.1"
                     /translation="MVISLKPEHLKRYKDIAVLFMKYGRSDLVKTSGLSLALGDDSPE
                     VAATAPEAGELAADLERLGPTFIKLGQLLSTRADILPPPYLEALTRLQDHVEPFSFAD
                     VERIVEDELGVRLSKAFNRFDSVPMASASLGQVHRAEMRDGREVVVKIQRPDIREQIV
                     QDVAALEELAVFLDSHTEMGKRYDFAQMLSEFRKSLLRELDYRDEAGHLTEFAENLRD
                     FDLLLIPRPVEDFTTSRVLTMDYIHGKKITALHPLRRLEINGTRLAEQLFRAYLQQIL
                     VDGVFHADPHPGNVFLTEDERVALLDLGMVARIMSAFQDNLLRLLLAISDGRGEEAAE
                     ITIRMGTRKENFEEEEFTRDVSDIVARHTDTSMNKVDAGRVVLEIQRIAAERGFRLPP
                     EFTMIAKALLNLDQVVYTLAPNFDPNAVIRRYASEIMQQRILKSLAPGNLMTNLLDVK
                     DFLERLPNRVNKILDAVGNNELELKVDAIDEKKLMEGLQKVANRITVGLVIAAMIIGA
                     SLLMRVETSFKLFGYPGLAIIFFLLAAAAAIWLIVVILFYDETAEK"
     gene            complement(7234..8475)
                     /locus_tag="VGB76_12365"
     CDS             complement(7234..8475)
                     /locus_tag="VGB76_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY0099736.1"
                     /translation="MTERCGSCGAELFEGQQFCRACGTPTRQFSSEDLPTQLLPHQQQ
                     QPPPPVQAPAPPQTPLSTAPLSGGRTTSDPLYGAGYPSSYQPPVVMPPQQQRHTSPVV
                     AGAPHAPRRRSRGWLYAVIALIIFFGGASLVAGLFFANRSQQRVTRVVRQKGVVPVPP
                     VPPPMPHPGVEAGDEDVLLENEGAEVSGNQTTITKTYPLAKNGTFAVHNLSGDITIEG
                     WDEQQAEVRIIKRGGSPQARDALRIMREQSPNRLVFNTMPEMRAGGVEEVRYEVKLPR
                     NLRELQIVSNHSNVELTGASATSISVVVQRGNIGLEDVSGTVNSRTTTGNTKVTIGET
                     ARTAPQVFNGVSGNIELEIAPDVNAELKAETVSGDIEVDEEFGIKVEKRMVGQQAAGR
                     IGKGGQPIVIKTVSGNIKIKS"
BASE COUNT         1686 a         2662 c         2518 g         1778 t
ORIGIN      
        1 gtttttcatg gctcacaatt cttccggcgg cacacgcctt tcacatctcg acacatacgc
       61 gaccgatccg gtcggcctcc acgtcatcat cgacacgccg cagggcagcc gcaacaagtt
      121 tgagtatgac gacgaactcg gcctcttcaa actcggcggc gtgctccccg ccggagcggt
      181 ctttcctttc aacttcggct tcgtgccttc gacgaagggc ggcgacggcg acccgctcga
      241 cgtgctcgtg ctgatggagg aagcggcctt cgccggttgc ctcgtcgtcg cgcgcctcat
      301 cggcgtcatc gaggcgaagc agaccgaaga ggacggcgag acggtgcgca acgaccgcct
      361 catcgccgtg gccgagaagg cgcgcaatca caaagaggtg cgctcgctcg gccaactgaa
      421 cgagcacttg ctggacgaga tcgagcactt cttcgtctcg tacaatgaaa tgaaggggaa
      481 ggagtttaag cctctggggc gtttcggcac cgagcgtgcg gagcgtctga tcgaggaagg
      541 cgcgcgccgt tttcgtgcgg aggaacgcaa gcagcgtccg cgcgcgcaaa agtcgaggaa
      601 gagttcgggc ggcgcggcga agacgcggcg ttaagctgaa acgatgaaga aggaagggtg
      661 aacgatgaag gagaagacag aattcagaat ccagaagtca gaagaaaacc tgcgtgagtc
      721 ttaacttctg actcctgtat tctgtgttct gtattccggt tcatcgttca tcgttccgac
      781 tccatcgttc cacaaaaaga ggagaagccg cgacgcttta agagtgtcgc aggcttctcg
      841 ctcgtttaga gagatgtctg gtcgctttta gccgcgcggt tgctgttgcg gcgggcggcg
      901 tttcttcttg ttgcgcttgc gggcgagggc tgacttgagt ttcgccttct gtcccttcga
      961 catgaagaac gcatgtttct tcgcgtcttt gatgatctct tcggtcatca ccttgcgctt
     1021 gaagcgtctg agcgcgctct cgattgattc accttgattg acgttaacag ttgccatctt
     1081 atctcctgct ctcaattttc acgcgaccgc gtagctgaaa gtcgtccgcg cgaagctgct
     1141 gtacttattt gttaatcaaa aatttgctaa aggtcaagcg gcgacgaacg tgcgccgccc
     1201 ttagttgtcg gcgcgtccgt gcacgagccg ccgcatacgg tcggagaaga gcacgtcgat
     1261 cttcttcggc tcgacgagag ccatcacttc gcccagcccg taggtcgggt ggagcatggt
     1321 ctgcccggcg cgataggtgc gcgtccaatc gtagggcgtg ccggtttgcg cgccggagcg
     1381 tgtgccggtg gcctgtgcgt tgttcttgaa ggaactgcgc gtgccgcact tcggacaatt
     1441 aacgcgcgaa atcaacccgc gcttcgtcac cgtggcgacg acgtgcccgc gatcttcgcc
     1501 gcaagcctcg caccatttct ccacgcggtc gccgacggca taagtctgtt tatccattaa
     1561 aacctccgca ataaccgtga atcgtggatc gtgaatagaa gtaaaccggg gtttctctat
     1621 tcaggattta caattcacgc tcttacttgt accggtaagt gatccgacct ttggaaagat
     1681 cgtaggggga catttcgacc tgcacgcggt cgccgggaag aatgcgtata aaatttttgc
     1741 gcatcttgcc gccgatttgc gccagcacga tgtgttcgct gttatcgaga cgcacgcgga
     1801 agaaagcgtt gggcagttta tcaaccacca cgccctcggc tggaatcaga tcatctttag
     1861 ccaaacagag tctcctttgg ttaaatcaag cgcgaacgcg agcgcgtgtg agagtcgccg
     1921 cgcgtaacgc tttacccgaa gtcggcgaac gaattgaccg gaaagtttcg tcgccacgtc
     1981 ttacccgcaa ccttgtaaaa aacctatcga accttgtacg tcgcgagcgc cccggcgacc
     2041 gtcgaaatca tcgcgcgggg cgcttctgcg atacgcttct caaatgagat ttcgccggaa
     2101 gctgctctgc cggagcgcgt ctaccagcgc ggctcgcggt cgccgccgcc gccaccataa
     2161 ccgccaccgc cgccacgatt gccgccataa ccgccgccac cgccgcgatt accgccacca
     2221 ccgccgccgt agccgccgcg tccgccacca ccgccaccgc caccgcgatc ttcacgcggg
     2281 cgcgcttcgt tgacgctcag gttgcgaccg ttgaattctt tgccgttaaa ctgctcgatg
     2341 gccttctgtc cctcttcgct cgaagacatt tcgacgaagc cgaagccgcg cgagcgtccc
     2401 gtatctctgt ccatcacgac cgaagcggac tccaccgtcc cgacctgccc gaacagttcc
     2461 tgtaaatctt cgccggaagt tttgaaattg agattaccga cgtaaagttt catgctcata
     2521 actgaaaatc tcctcctcct tgaggaagcg tgaagagaag ctcgaatgct attggcttcg
     2581 tgaacgggta gcagcgaagt cgcgtgttcg gaatgccctg acgagcagct tctgcaaatt
     2641 aataccggcc tgctctcgaa ctatccagac gccgccgctt ggttctccac agcgcggggc
     2701 gcatcaccgt tgatgcgcga gagaaggagt ggacggcaga tattatgcct tgtgccggaa
     2761 aaaaaggcaa aagccgagtg gcgacggggc aaaacggcaa cagggcgaac gccgcgccgc
     2821 attggggcag aattacccac gccccgcacg cctcggctga actctcgcgg cgcgaaatcg
     2881 ccccgcactt ttgacataca cgcttgcacg gtaatactct taccccgcgc ccacagagaa
     2941 gttcgacgcg ccccgcgccc gttgatttac aataaccgtg cacaaactcg acggtgattt
     3001 cgcagacgct cccgcagacc cttcgcgcgc tgcttaaccc ggcgacctga cctcaaacgc
     3061 cgtgcggatg cgcgtgcgcc gccgcgattt tctttcagtt gacagccgcg cggcatcgtt
     3121 ccggttgcgg cgacagtcaa cgccggtcaa cttttcgtct ctttcgcctg caacgaatta
     3181 cggcataaac tttgccacac gagcgttcgc aacgttcctc cattttccgg attgcgagat
     3241 ttggcagcac catgaaattc aaaaccctcg tcctcgccgt ttccacgttc ttcatgcttg
     3301 tagcaattcc cgccgccgca caggggcaga cgtggcaata caccgcgctc ggcgattcgc
     3361 tcggagccgg actctacgac tcgcagggcg gctacgtccc gcgcttccgc aactacgtgc
     3421 aggccgacac gggcgtaaac gtcatactca acaatcgcag cgtctccggc tggaaggcca
     3481 cggatttgct gcacgcgcta cgcaccgacg agagcctgcg cacgcagatc gccaactcgc
     3541 agatcgtcac ctggaacatc ggcgcaaacg attttcagga cgcgctccta cgctatcaga
     3601 acggcacatg cggcggcgcg gacaatcaga attgcatacg cgcggcggtc gccaagttga
     3661 aggttgactg gaacgccatc gtcgcgcaga tactctccct gcgcagcccc tcgaacacca
     3721 tcctgcgcac gatggacaac tacaatccgg cggttgatct ggcacgcgcc ttcggcgtgc
     3781 tccacgtcat caagccgtat ctggaagagg tcaaccgctt catcttcctg agcgcgatca
     3841 acaatcgcat cggctgtgcg cgcgtctatc aagccttcaa cggcgtgaac ggcgaagcgg
     3901 acgccgggcg gcgcggctac atgtcatccg acggcataca cccgaacgaa ctcggccacg
     3961 acatcatcgc ttccgagttt cgcgcgctct cttacgcgcc ccttgtcgcg ccgcaaagca
     4021 cgatcagcct gttgctcccg gtcaccatcg tcaacgaggg cgacgggcgc gccaccgtca
     4081 ccgtcacgcg cggcggcaac acgagcggcg cggcttcggt cgagtatgcg acgcttgata
     4141 atccggctga cgttccctgc gccgcgccgc cttcgagcga cgactcggtc agcatcgctt
     4201 ttgcacgctg cgactacgcg accaccgttg atgcgctgca tttcgccgcc ggagagacgc
     4261 agaagagtct caacattccg ctcatcaacg atgcccacgt cgaaaacaca gaggagtttt
     4321 ccatccggct cacgggcgcg tcgggcgcgt cgctcggcgt ccgcacgtcc gccgtcgtgc
     4381 gcatcatgga caacgaagcc cgcgtcacgc cgcccctcgt cttcccgaat ccgattcaca
     4441 cgagcgaatt cttcgtccgc atgcagtatc tcgactttct ctcacgcgag gccgaacccg
     4501 caggcttcgc cgcgtggatg aacgtcctca acaattgctc agacctgcat ctcaatcccg
     4561 cgtgtgaccg caccacggtt accgcctcct tcttccgctc tgaggagttt aatctcaaag
     4621 cctacttcgt ctatctcttc tataagacca cgctcggcag gctgccgcgt tacacggaga
     4681 tcatccccga catgcgccgc gtttcaggag aaaccgccga agaagttttc gcgcgacggc
     4741 tcgcgttcgc ctatgcgtgg gtggagcgcg tggaatttaa gagcctctat ccggagacgc
     4801 tttcgcagga agagtttgta gacaggttgc tgcaagccac gggcgtcggt ttgagcggga
     4861 gcgtgtcgcg caactcgctc gtgagcgatc tcaacacgag aaacaagacg cgcgccgaag
     4921 ttctcatcgc gctcgtcgag catcctgcgg ttgacttgcg agagcgcaac ggcgcattcg
     4981 tcgccatgca atacttcggc tatctgcgcc gcgacccgga cacgggcggc tacaacaact
     5041 ggctcaacta tctcaacgcc aacccgaacg acttccgcac catggtcaga ggcttcgcca
     5101 actcgcagga gtatcaactg cgcttcggac aagcacaatg atggaaggga tgaaggatga
     5161 gggatgaggg atgaatcaga aaagcgttca actttcttga atgctccttc ttattcatcc
     5221 ctcatccctc atccttcatc cctcatcctt catccctcta tttctccgct gtttcgtcgt
     5281 agaagagaat gacgacgatg agccagatgg cggcggcggc ggcgagcagg aagaagatga
     5341 tggcgaggcc gggataaccg aagagtttga agctcgtctc gacgcgcatc aggagcgacg
     5401 cgccgatgat catggcggca atgacgaggc cgaccgtgat gcggttcgcc accttctgca
     5461 aaccctccat caacttcttt tcgtctatcg catcaacctt caactccaac tcgttattgc
     5521 cgaccgcgtc taaaatcttg ttgacgcgat tgggcaggcg ttcgagaaag tccttcacgt
     5581 cgagcagatt ggtcatcaag ttgcccggcg cgagcgactt caggatgcgc tgctgcatga
     5641 tctcgctcgc gtagcggcga ataaccgcgt tcgggtcgaa attgggcgcg agcgtgtaga
     5701 ccacctgatc gagattgagc agggctttgg cgatcatggt gaattcgggc gggaggcgaa
     5761 agccgcgctc agccgcgatg cgctggattt cgagcacgac gcgcccggcg tccaccttgt
     5821 tcatgctcgt atcggtatgg cgtgccacga tgtccgacac gtcgcgcgtg aactcttctt
     5881 cttcgaagtt ctctttgcgc gtgcccatgc ggatcgtgat ctcggcggct tcctcgccgc
     5941 gcccgtcgct gatcgcaagg aggagcctca gcagattatc ctgaaaggcc gacatgatgc
     6001 gcgcgaccat gccgagatcg agcagcgcga cgcgctcgtc ttcggtgagg aagacgttgc
     6061 cggggtgcgg gtcggcatga aagacgccgt ccacgagtat ctgttggagg taggcgcgga
     6121 agagctgttc ggcgagccgc gtgccgttga tctccagacg gcggagcggg tggagcgcgg
     6181 tgatcttctt gccgtggatg tagtccatcg tcaggacgcg cgaggtggtg aagtcttcga
     6241 cggggcgcgg gatgagaagg agatcgaagt cgcgcaggtt ttcggcaaac tccgtgaggt
     6301 gcccggcctc gtcgcggtaa tcgagttcgc ggagcagact cttgcgaaac tccgagagca
     6361 tctgcgcgaa gtcgtaacgt ttgcccatct ccgtgtgcga gtctaaaaag acggccagtt
     6421 cttcgagcgc ggccacgtcc tgcacgatct gttcgcggat gtccggacgc tgaattttga
     6481 ccaccacttc gcgcccgtcg cgcatctccg cgcggtgcac ctgccccagc gaagccgacg
     6541 ccatcggcac actgtcgaag cgattgaacg ccttcgacaa ccgcacgccg agttcgtctt
     6601 cgacgattcg ctcgacatcg gcgaaagaga acggctcgac gtgatcctgc aagcgcgtga
     6661 gagcttccag atagggcggc ggcaaaatgt ctgcgcgcgt ggagagcagt tgtccgagtt
     6721 tgatgaaggt tggacccagc cgttcgaggt cggcggcgag ttcgcccgct tccggcgcgg
     6781 tcgcggccac ttcgggcgag tcgtcgccga gcgcgagcga gagtccgctc gtcttcacga
     6841 gatcggagcg gccatacttc ataaacagca cggcgatatc tttgtaacgc ttcagatgtt
     6901 cgggcttcag cgagatgacc atcaaatatt cctcggacgg gaaacttatc ctagacttga
     6961 agacttgaaa ctttgggaga gagccgcgct tatgcagcga cgaattgatt ataccgattc
     7021 aatcgccgga cgccactcga tattaagcgg cgaacggatg cgggcaaaca aacctgcaac
     7081 gccgcagaca gcagagagga acgatgaggg ggcgcagaga agatattgag tccttctctg
     7141 cgcccccttc atcgttccgg ttctcgcgtc ggcggcggcg agccttactt ttcgacgacg
     7201 ctctcagcgc aactcaggct aaatttcgtc gcttcaactt ttgatcttga tgttgccgct
     7261 caccgtcttg atgacgatgg gctgcccgcc tttgccgatg cgcccggcgg cttgctggcc
     7321 gaccattcgt ttctcgacct tgatgccgaa ctcttcgtca acctctatgt cgccgctgac
     7381 ggtttcggcc ttgagttcgg cgttcacgtc cggcgcgatt tcgagttcga tgttgccgct
     7441 gacgccgttg aagacttgcg gcgcggtgcg ggcggtttcg ccgatggtga ctttggtgtt
     7501 gcccgtcgtc gtgcgcgagt tgaccgtgcc gctcacgtct tcgagtccga tgttcccgcg
     7561 ctgcacgacg accgagattg acgtggcgct cgcgcccgtg agttcgacgt tcgagtggtt
     7621 cgagacgatc tgcaattcgc gcaggttgcg cggcagtttc acctcgtagc gcacctcctc
     7681 gacgccgccc gcgcgcatct ccggcatcgt gttgaagacg agccggttcg gactctgctc
     7741 gcgcatgatg cgcaaggcgt cgcgcgcctg cgggctgccg ccgcgcttga tgatccttac
     7801 ttcggcctgt tgttcatccc agccttcgat cgtgatgtcg ccgctcaggt tgtgtacggc
     7861 gaacgtgcca tttttggcga gcgggtaggt cttcgtgatc gtggtctggt tgccggaaac
     7921 ttccgcgccc tcgttttcga gcaacacgtc ctcgtcgccc gcttccactc ctggatgcgg
     7981 catcggcggc ggaaccggcg gaaccggcac gacgcccttc tgccgcacga cgcgcgtgac
     8041 gcgctgctgg ctgcggttag caaagaagag tccggcgacg agcgacgcgc cgccgaaaaa
     8101 gatgatcaag gcgatgacgg cgtagagcca gccgcgcgaa cggcggcgcg gcgcgtgcgg
     8161 cgcacccgca acgacgggcg acgtgtgccg ctgctgctgc ggcggcatga cgacgggcgg
     8221 ttgataggat gatggataac ccgccccgta gagagggtcg gaagtggtgc gcccgccgga
     8281 gagcggcgca gtgctgagcg gcgtctgcgg cggcgcgggt gcctgcaccg gtggtggtgg
     8341 ttgctgctgc tggtgcggga gcaactgcgt ggggaggtct tcgctggaaa actggcgcgt
     8401 cggcgtgccg cacgcgcggc aaaactgctg cccctcgaac aactccgcgc cgcaactacc
     8461 acatcgttct gtcatacgtc accgccaccc gctactaagc cagagattta tttgtccgaa
     8521 cactttcgca cgcttgattc tatccgcgcc catacgcttt tcacaacacg aaaaaaattt
     8581 tcgattaaga gacgagccgg agactcccgc acttacccgc gcgcactcta gctgtaaacg
     8641 ctgg
//
LOCUS       DASWNS010000352        10406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_402347, whole genome shotgun sequence.
ACCESSION   DASWNS010000352 DASWNS010000000
VERSION     DASWNS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402347"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..4152
                     /locus_tag="VGB76_12370"
     CDS             277..4152
                     /locus_tag="VGB76_12370"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0099737.1"
                     /translation="MKIKSLPQILLCLIMLTSGAGSAWAQGTASRVTGTVVDQSGAII
                     PDATVTLTNEATKVDFTAQTTTTGNYVFDSVQVGVYTLSVEKQGFKKFVSTTNQVNVN
                     QPATIDVAMEAGGLNEVVTVEASAELVQTSSSGNFGNTVEERTLTTLPIVGSRGRNPL
                     SFILFQPGVTPDANTGGGVHVHGSRDRAFNFTLDGIDINDTTAGGSNFTPVRANPDSL
                     SQFQVVTGNFTAELGRSSGAQVSLITKSGTNEFHGNLFEFYQTPRFHANEYQNGINTV
                     LVGGQRVPTIKPQFVQHIFGGSIGGPVYLPSFGEGGDRVYNGKNRTFFFTNLQLLRTS
                     QTILRNRTVYTAQARAGLYRYIVNGQNGNLLSTTASGPSIDANGNPVYAPCPAGVPTA
                     STPRCLATFNVANNPNIPLSFDPTTQRLIGLTPLPNNFTLGDGLNTAGYTFVAPQTER
                     QYDFTTKIDHNFNDRHAMYVRYSRGAQNTLGDNANVGLQAFPGLPNIVDTFRTPRNLA
                     VNYRATLTPNLVNEFVAGFNRFTFSFNNPDPNAAINSPVILNNVTDPLNASPSVNNAR
                     KITTRQFVDNLSWAKNAHTFKFGTNIRLQQHIDDRSSVAGTNTRETVLLSTSGTTGNP
                     VPASFGTTSNTARINATDRTRLDSYVNDIFGRIGSISQGFVANEAGTAFAPAGERFLY
                     DARYPELDFYAQDTWKLRPNLTFDYGLRWEVRLSPRAPNDIVLRPSTPVRIGASPTNA
                     LTFEEGKLYEDQYKQFAPSVGFAYDPFGTGKTSIRANYRLAYDRTNTFVFSSFIFQSA
                     PGLTRGITVRATDPIFGGANNALLRNGLPTLNANDRTPQQFRQPPAFSVNTLTVVDPN
                     LSYPRTHQYGISLQREVGGGNVLEVNYIGRQGRKLYGGYDVNQMDIDNNGFLAAFRQL
                     QDPATRASVITNPNFLINQLLAGDTRLGTVNLPGGGTRAETGAELLLRQSAGPVRLAS
                     GAFANNIVDAGGVANAAVFINSGVRSSNTSVPVFQANNFSPFFFQPFPQFSGAVNVLD
                     NNDESHYNALEIQISRRSRNGFGYQASYTLAKSTDTRSFDPTFAIANRGNAQSTANTP
                     FDIRDRKRNLARSDFDRRHALQGYFTYELPFGRGRRFLADSKGFVDRLVGGFELAGIV
                     RVYSGRPFTVYSGINTLSNVLNSPASCNGCTPDMGRLQLNTSGRNAFFTAEQLAQFYT
                     PAAGELGNTGRNFFTGPNYFQLDMTVGKRIRFTESLNLELRMEAQNATNTPIFDFPTA
                     VITSTSFGTINDLVLSSSRKVQFAAKFNF"
     gene            4224..5873
                     /locus_tag="VGB76_12375"
     CDS             4224..5873
                     /locus_tag="VGB76_12375"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEY0099738.1"
                     /translation="MSRNFLRPRAAALALLLAAVLAAFASTLQQAQTNPDAKLPAYDL
                     VITNARVVDGTGNPWFRADIGVRDGRIARIGRIAGGEAQNVVDARGQIVAPGFIDVHT
                     HVENIYNLPAAENFVRMGVTSVVTGNCGSSATDIGAFLGRLKEQPLAVNLATLVAHGD
                     VRRKVLGLEARAPSAEELQKMEAIVEQGMRDGALGLSTGLIYVPGTFAKTDEVTALAR
                     VAARHGGVYATHMRSEGEHVLAAIRESIEIGERAGLPVEISHFKISNKKLWGQTASTI
                     GLVREARGRGLQVTVDQYAYTASSTSLDSRLPSWALEGGRAAGKARLADKATRERVVG
                     EIKDSLKRSGFKDFSYAVVASYAPNPAFNGKSIAEITKVARGKTDVTNQIEQILEMYA
                     AGGAGMVYHGMSEPDVQNIMREPFTMIASDSGVRRFGDGAPHPRGYGNNARVLARYVR
                     ELRLLTLEDAIRKMTSLPAQTFRLAGRGLLREGYAADLVIFDETTIADRATFDAPHQY
                     PTGISHVFVNGQRVFAAGEMTPARPGEALRSANWVGAPASP"
     gene            6063..9560
                     /locus_tag="VGB76_12380"
     CDS             6063..9560
                     /locus_tag="VGB76_12380"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0099739.1"
                     /translation="MINQFHKISRSVLAVMLLLACAGSVGYAQEITARISGQITDAAG
                     AVVPNSEVTLTNKNTGESRQAQSDADGYYTLTLIQPGTYDLSVKVQGFKEHLSTGLEV
                     SVNDRKTINIALEAGSVTETVTVTGEAPLIQTTATVGDVVENRRVVELPLNNRNFMQL
                     LTLVPGVTSSGDSEIGIGLTNTVNFSINGTRRNSINFLVDGVTNSDVGSNITLLSVPT
                     VDSIQEFRVITSVPSAEFGRSGGGVVNLITRGGGREFHGTVYEFLRNDYLNANSFFNN
                     ANGTFGPNDAQVLNGARQVGDERSPRAKLRYNNFGYLVSGPLFIPRVYNTEREKTFFL
                     FSQEFRRIIRAPAQSTVTVPSLLERQGNFSEAGNLPIFDPLTGTRFPNNVIPTARLDP
                     VALSLLNLFPEPNVASATPGRAPNRYAVVAPNIQNTRQETVRIDHNFSDTHRMTGRYT
                     RDLSQTREQGGLFFGATLPNLATTDTSVPGEVMAISLTSSFGPNIVNEAQFTFSGNKI
                     TTDLVGRYTRANANIPNRELFPENNSGLPPIINITGQPTLGSGQLYDVQYKNWNPKDN
                     VTIIHGAHTIKFGGEVSFESKRENASNSAQGTFGFTGVQTRLGNNTGIGLADFLLGRA
                     TSYAEPERDVTVDLRFGRTEFYVQDTWKVRPNFQLDLGIRYYRFRQASDENNVLATFL
                     PQFYNPARAPQCANATCSTFVTSSFDLTNGFAYAGINSPYRHRVQRPDKNDWGPRAGF
                     SWDPFNNGKTVLRGGYGVYYDQALVGIVEQNSFTTPPFNNSISLTGTVASPILYQNPT
                     AGTAATTRGPLGTVNATTDPFYTPLIQQWSLTVQQEPFRNALFEIGYSGSAGNHLIRP
                     VDINAPTPQEIIAAGRGIATCDQALNPANNPANCINLARPYRGFGIITDRQTTATFRY
                     HGMLSSFRLRPTRGLTAQLSYTWSKNMTDATNDRDAIDVPQIRTNFQLERAVSRLDRT
                     HVFVASYVYEVPTFSSGFASTTFGRLLLSGWELAGITTAQTGLPLNRVVQGSATIPAR
                     GSRPNIIGDPMQNIPVNPSGGIPYAFNPLAFRPTNTGEIGNSPRSPLRFPNQFFTDLN
                     VTKNLRFTERYRVQLRAEFFNIFNHAIFNDVFQTIPDRSPTDPAFNSIQNLAAVSQFG
                     QFFSTRNPREIQLGLKFYF"
     gene            complement(9690..>10406)
                     /locus_tag="VGB76_12385"
     CDS             complement(9690..>10406)
                     /locus_tag="VGB76_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099740.1"
                     /translation="FEEAAAELEISVALNPNDAASLAALGETYLRLKEYRKAVPHLER
                     SIRLQPEGGAAKARLRLGEALDNAGESERAIEELRRAVAADPKLPEAHYALGYALLKR
                     GNLDEAVAQFEQELELNPGMLLARYQLGNVYFQKGRLEEAVALLNKVLEVEPNFRPAL
                     ETLGQALLKQGAATEAVRVLERAVKLDPEWPQGFALLGRAYMSAGKRGDARQAFATAQ
                     RLGQEERKRLEEKVSQPKKN"
BASE COUNT         2065 a         3554 c         2886 g         1901 t
ORIGIN      
        1 agttcatata tagttcattt acaatacgcc gcagttagcc caagggtcag tagtttccgt
       61 ccttcatcgt gttttcaggc aaagcgtaca agcggggtgg ttgagaggtt cgtgccacag
      121 gtgcgcgcct ctcccgaacc accgcgagac ttccaccgcg cgcaagtgaa tttacaattc
      181 gagcgtttat gtccggcgag cgagtctcca cagtctctct aacgcctgtc gtgcaggaac
      241 gcccaaacct tttgtcggaa ctaaggagcc gagttcatga aaattaagag tctcccacaa
      301 atcttgctat gcctcattat gttgacgagc ggagccggca gcgcgtgggc gcagggcacg
      361 gcttcgcgtg tcaccggtac ggtcgttgac cagagcggcg cgatcattcc cgatgccacc
      421 gtcaccctca ccaacgaagc cacaaaagta gacttcaccg cgcagaccac gacgaccggc
      481 aattacgtct tcgattccgt gcaggtcgga gtctatacct tgtcggtgga gaagcagggt
      541 ttcaaaaaat tcgtctccac cacgaatcag gtcaacgtca accagcccgc tacgattgac
      601 gtggcgatgg aagccggggg cttgaacgag gtggtgactg tcgaggcttc cgccgaactc
      661 gtgcagacga gcagttcggg caacttcggc aacacggtcg aggagcgaac tctgaccacg
      721 ctgcccatcg tcggctcgcg cggtcgcaac ccgctcagtt ttattctctt ccagcccggc
      781 gtcacgcccg atgccaacac gggcggcggc gtccacgtcc acggttcgcg cgaccgcgcc
      841 ttcaacttta cgctcgacgg cattgacatc aacgacacga cggcgggcgg ctcgaacttc
      901 acgcccgtgc gcgccaaccc cgattcgctc tcccagtttc aggtggtgac cggaaacttt
      961 accgccgaac tcggtcgctc gtcgggagcg caggtcagcc tgatcacgaa aagcgggacg
     1021 aacgagtttc acggcaacct cttcgagttc taccagacgc cgcgcttcca cgcgaacgaa
     1081 tatcagaacg gcatcaacac ggtgctcgtc ggcggtcagc gtgtgccgac catcaagccg
     1141 cagtttgtgc agcacatctt cggcggctca atcggcggcc ccgtctatct ccccagtttc
     1201 ggcgagggcg gcgaccgcgt ctataacggc aagaatcgca ccttcttttt caccaacctg
     1261 caactcctgc gcaccagcca gaccatcctg cgaaaccgca cggtctatac cgcgcaggcg
     1321 cgcgccggac tctatcgcta catcgtgaac gggcagaacg gcaatctgct ctccaccaca
     1381 gcctccggcc cttcgattga cgccaacggc aatcccgtct acgcgccctg tcctgccgga
     1441 gtcccgaccg ccagcacgcc cagatgtctg gcgaccttca acgtggcgaa caacccgaac
     1501 atcccgctct cgttcgaccc gaccacgcag cgtttgatcg gcctcacgcc gctgccgaac
     1561 aatttcacgc tcggcgacgg cctcaacacg gcgggctaca ccttcgtcgc gccgcagacc
     1621 gaacgccagt atgatttcac caccaagatc gatcataatt tcaacgaccg ccacgccatg
     1681 tacgtgcgct actcgcgcgg cgcgcagaac acgctcggcg ataacgccaa cgtcggtttg
     1741 caggctttcc ccggactgcc gaacatcgtg gataccttcc gcactccgcg caacctcgcc
     1801 gtcaactatc gcgcgaccct gacgccgaac ctcgtcaacg agttcgtcgc aggcttcaac
     1861 cgcttcacct tcagcttcaa caatccagac ccgaatgcgg ccatcaattc gccggtcatc
     1921 ctgaacaatg tcaccgaccc gctcaacgct tcgccgagcg tcaacaacgc gcgcaagatc
     1981 acgacccgcc agtttgtaga caacctgagc tgggcgaaga acgcgcacac gttcaagttc
     2041 ggcacgaaca tccgtctcca acagcacata gacgaccgca gttcggtggc gggcaccaac
     2101 acgcgcgaga cagtcctgct ctccaccagc ggaaccacgg gcaaccccgt cccggcctcc
     2161 ttcggcacaa cttcaaacac ggcacgcatc aacgcgacgg atcgcacgcg tctggatagt
     2221 tacgtgaacg acatcttcgg gcgcatcggc tcgatctcgc agggcttcgt cgccaacgag
     2281 gcgggcacgg ccttcgcccc ggcgggcgaa cgcttcctct acgacgcgcg ctatcccgaa
     2341 ctcgacttct acgcgcagga cacctggaaa ctgcgtccca acctgacgtt tgactacggc
     2401 ctgcgctggg aagtgcggct cagcccacgc gccccgaacg acatcgtcct gcgcccttcc
     2461 acgcccgttc gcatcggcgc gtccccgacc aacgcgctca ccttcgaaga aggcaagctc
     2521 tacgaagatc agtacaaaca gttcgccccg tcggtcggct tcgcctacga tccgttcggc
     2581 acgggcaaga cctccattcg tgccaattat cgtctggcct acgaccgcac gaacacgttc
     2641 gtcttctcgt ccttcatctt ccagagcgcg cccggcctga cgcgtggcat cacggtgcgc
     2701 gccaccgatc ccatcttcgg cggcgcgaac aatgctctgc tgcgtaacgg cctgccgacg
     2761 cttaacgcga acgaccggac gccgcagcag tttcgccagc cgcccgcctt cagcgtcaac
     2821 acgctgaccg tcgttgatcc caacctcagc tacccgcgca cgcaccagta cggcatcagc
     2881 ctccagcgcg aagtgggcgg cggcaacgtg ctcgaagtca actacatcgg gcgtcagggg
     2941 cggaagctct acggcggcta cgatgtcaac cagatggaca tcgacaacaa cggcttcctc
     3001 gcggccttcc ggcaactgca agaccctgcc acacgcgcct ccgtcatcac caacccgaac
     3061 ttcctgatca accaactgct cgcgggcgac acccggctcg ggacggtgaa tctgccgggc
     3121 ggcggcacca gagcggaaac gggcgcggag ttgttgttgc gtcaaagcgc ggggccggtg
     3181 cgtctggcga gcggtgcgtt tgcgaataac atcgtggacg cgggcggcgt ggctaacgcg
     3241 gcggtcttta tcaactccgg cgtgcgttca agcaacacga gcgtcccggt ctttcaggcg
     3301 aacaacttct cgcccttctt cttccagccc ttcccgcagt tttccggcgc ggtcaacgtc
     3361 ctcgacaaca acgacgagtc gcattacaac gcgctcgaaa tacagatcag ccgccgctcg
     3421 cgcaacggct tcggctatca ggcgagttac acgctggcga agtcaaccga cacacgctct
     3481 ttcgacccga ccttcgctat cgccaatcgc ggcaacgcac agtccactgc gaacacgccc
     3541 tttgacatcc gcgaccgcaa acgcaatctc gcgcgctccg atttcgaccg ccgccacgcg
     3601 ctgcaaggct acttcaccta cgaacttccg ttcggcagag gtcgccgttt tctcgcagac
     3661 tcgaagggct tcgtagatcg tctggtcggc ggctttgaac tggccggaat cgtgcgcgtg
     3721 tattcgggtc gcccgttcac ggtctattcc ggcatcaaca ccctgagcaa cgtcctgaat
     3781 tctccggcca gttgcaacgg ctgcacgccc gacatgggca ggctccagtt gaataccagc
     3841 ggcaggaacg ctttctttac cgccgaacaa ctcgcgcagt tctacacacc tgcggcgggc
     3901 gaactgggaa acaccgggcg aaacttcttc accggaccca actatttcca actcgacatg
     3961 accgtgggca agcgcatcag gttcaccgag tcgctcaatc tcgaactgcg catggaagcg
     4021 cagaacgcga ccaacacgcc catcttcgac ttcccgaccg ccgtgattac cagcacgtcg
     4081 ttcggcacca tcaacgacct cgtgctcagt tcttcccgca aagtgcagtt cgcggcgaag
     4141 tttaacttct aaaccagaac gcggcggcag cgcgtcgaga tagacgcgct gccgcccttt
     4201 aaccttccga aaaagacgtt tcgatgtcga gaaactttct ccgtcctcgc gctgctgcgc
     4261 tcgcccttct gctggcggct gtgctcgccg cgtttgcctc gacgctccaa caggcgcaga
     4321 cgaaccccga cgctaaattg cccgcctatg atctcgtcat cacgaatgcg cgcgttgttg
     4381 acggcacggg caatccctgg tttcgcgcag acatcggcgt gcgcgacggg cgcatcgcgc
     4441 gcatcgggcg catcgcaggc ggcgaggcgc aaaacgtcgt cgatgcgcgc ggccagatcg
     4501 tcgcgcccgg tttcattgac gtacacacgc acgtcgaaaa catttacaac ctgcctgccg
     4561 ccgaaaactt cgtgcggatg ggcgtcactt ccgtcgtaac gggcaactgc ggctcgtccg
     4621 cgacggacat cggcgcgttc ttaggtcggc tcaaggagca gccgctcgcc gtcaatctcg
     4681 ccacgctcgt cgcgcacggc gacgtgcgcc gcaaagtgtt gggactcgaa gcgcgcgcgc
     4741 cctctgccga agagttgcaa aagatggagg ccatcgtcga gcagggaatg cgcgacgggg
     4801 cgttggggct ttcgaccggg ttgatctacg tgcccggcac cttcgccaag acggacgaag
     4861 tgaccgcgct cgcacgcgtg gccgcgcgcc acggcggcgt ctatgccacg cacatgcgca
     4921 gcgagggcga acacgtcctc gctgccatcc gcgaaagcat cgagatcggc gagcgcgccg
     4981 gattgcccgt agagatttcg cacttcaaga tcagcaataa aaagttgtgg ggacaaactg
     5041 cgtccaccat cggcctcgtg cgcgaagcgc gcgggcgggg gttgcaggtc acggttgacc
     5101 agtatgctta cacggcgagt tcgacctcgc tcgattcgcg ccttccgagt tgggcgctcg
     5161 aaggcgggcg tgcggcgggg aaggcgcgcc tcgcagacaa ggccacgcgc gaacgcgtcg
     5221 tcggcgaaat aaaagattcg ctcaagcgga gcggcttcaa agacttctcc tatgcggtgg
     5281 tggcgagcta cgccccgaat cctgcgttca acggcaagtc catcgcggag atcaccaaag
     5341 tggcgcgtgg caagacggac gtgacgaatc agatcgaaca gattctggag atgtacgcgg
     5401 cgggcggcgc ggggatggtc tatcacggca tgagtgagcc ggacgtgcag aacatcatgc
     5461 gcgagccgtt cacgatgatc gcctccgaca gcggcgtgcg ccgctttggc gacggcgcgc
     5521 cgcacccgcg cggctacggt aacaacgcgc gcgtgctcgc acgctacgtg cgcgaattgc
     5581 gcctgctcac cctcgaagat gcgatccgta aaatgacttc gcttccggcg cagacctttc
     5641 gcctcgccgg gcgcggcctc ctgcgcgaag gctacgccgc cgacctcgtt atcttcgacg
     5701 aaacgaccat cgccgaccgc gccaccttcg acgcgccgca ccagtatcca accggcatca
     5761 gccacgtttt cgtcaacggc cagcgggtct ttgccgccgg agagatgact ccggcgcgac
     5821 ccggcgaggc tctgcggagc gcgaattggg tgggcgcacc cgccagccct tgaggcggac
     5881 ttgagcgggc tgcgcgggtt gtgacaaaat aacgcgcaag caagttattc caacgtgcaa
     5941 tctgttccaa cgtcgaattg attccaagcc ttacttgaaa cgtctaggtc tacgagcgcg
     6001 gaggcttctg tctcctgcgt tcaacccggt ctgaattttg ttacgtgtct tagaggaaac
     6061 ttatgataaa ccagttccac aaaatctccc gcagcgttct ggccgtcatg cttctgttgg
     6121 catgcgccgg aagcgtcggg tacgctcaag agatcacggc gcgcatcagc ggccagatca
     6181 cggacgccgc cggggccgtc gtgcccaact cggaggtgac gctgaccaat aagaacaccg
     6241 gcgagtcgcg ccaggcgcaa agcgacgccg atggttatta cacgctcacg ctcatccagc
     6301 ccggcaccta tgatctgtcc gtgaaggtgc agggattcaa agagcacttg agcaccggtc
     6361 ttgaggtgag cgtcaacgac cgcaagacca tcaacatcgc gctcgaagcc ggttccgtca
     6421 ccgaaacggt cacggtgacg ggcgaagcgc ccctgattca aacaaccgcg acggtcggcg
     6481 acgtggtcga gaaccggcgc gtcgtcgaac taccgctcaa taaccgcaac ttcatgcaac
     6541 tgctcacgct cgtccccggc gtgacgagtt ccggtgattc cgagatcggc atcgggttga
     6601 cgaacaccgt caacttctcg atcaacggca cgcgccgcaa ctcgataaac tttctcgtgg
     6661 atggtgtaac caactccgat gtcggctcga acatcaccct gctctccgtg ccgaccgtgg
     6721 attcgattca ggagtttcgc gtcatcacgt ccgttccttc cgccgagttc gggcgttcgg
     6781 gcggcggcgt cgtgaatctc atcacgcgcg gcggcgggcg cgaattccac ggcaccgtct
     6841 acgaattttt gcgcaacgat tatctgaacg ccaacagctt ctttaacaac gccaacggga
     6901 ctttcggccc caatgacgcg caggtgctga acggggcgag gcaggtcggc gacgaacgct
     6961 cgccgcgcgc caagctgcgt tataacaact tcggttatct cgtcagcggc cccctcttca
     7021 tcccgcgcgt ttacaacacc gagcgcgaaa agaccttctt cctcttctcg caggagtttc
     7081 gtcgcatcat ccgcgccccc gcgcagagca ccgtcaccgt gccgagcttg ctcgaacgtc
     7141 agggcaactt ctccgaggcg ggcaatctgc cgatcttcga tcctttgacc ggaacgcgct
     7201 tcccgaacaa cgtgattccc acggcgcggc tcgatccggt tgcgctctcg ctcctcaacc
     7261 ttttccccga accgaacgtc gcctcggcca cgcccggacg cgcgcccaac cgttatgcgg
     7321 tggttgcgcc caacatccag aacacgcggc aggagacggt tcgcatcgat cacaacttca
     7381 gcgacacgca ccgcatgacc ggacgctaca cgcgcgacct cagccagacg cgcgagcagg
     7441 gcggcctctt cttcggcgcg acgttgccca atctggcaac caccgacacc agcgttcccg
     7501 gcgaagtgat ggcgatcagc ctgaccagca gcttcggacc caacatcgtc aacgaagcgc
     7561 agttcacctt ctccggcaac aagatcacga ccgacctcgt cgggcgttac acccgcgcga
     7621 acgccaacat cccgaaccgc gaactgttcc cggagaataa ttccggcctg ccgcccatca
     7681 tcaacatcac cgggcagccc acgctcggct ccggtcagct ctacgacgtg cagtacaaaa
     7741 actggaaccc gaaggacaac gtgaccatca ttcacggcgc gcacacgatc aagttcggcg
     7801 gcgaagtgag cttcgagtcg aagcgcgaga acgcttctaa ctccgcgcag ggcacgttcg
     7861 gtttcacggg cgtgcagacg cgcctcggca acaacacagg catcggcctt gccgatttcc
     7921 tgctcggtcg tgccacctcc tacgctgaac cggaacgcga cgtgacggtt gacctgagat
     7981 tcgggcgcac agagttttac gtgcaggaca cctggaaggt gcgacccaac ttccagttag
     8041 acctcggcat tcgctactac cgtttccgtc aggcgagcga cgagaacaac gtgctggcta
     8101 ccttcctgcc gcagttctac aatccggcgc gcgcgccgca atgcgccaac gcgacgtgca
     8161 gcaccttcgt cacgagttcg ttcgacctga cgaacggttt cgcttacgcg ggcatcaatt
     8221 cgccttaccg ccaccgcgtg caaaggccgg ataagaacga ttggggtccc cgcgcaggct
     8281 tctcctggga cccgttcaat aacggcaaga ccgtgctgcg cggcggctac ggcgtctact
     8341 acgatcaggc gctcgtcggc atcgtcgaac agaattcgtt caccacgccg cccttcaata
     8401 actcgatcag cctgaccggc acggtggcaa gcccgatcct ctaccagaac cctacggcag
     8461 gcaccgccgc aacgacgcgc ggaccgctcg gcaccgtcaa cgccaccacc gatcccttct
     8521 acacgccgct catccagcag tggtctttga cggtgcagca ggagccgttc cgcaacgctc
     8581 tcttcgagat cggctactcc ggctcggcgg gtaatcacct gattcgtccc gtcgacatca
     8641 acgcgccgac tccgcaggag atcatcgccg ccgggcgcgg catcgccacc tgcgatcagg
     8701 cgctcaatcc ggcgaacaat ccggccaact gcatcaacct cgcgcgtccc tatcgcggct
     8761 tcggcatcat caccgaccgg cagacgacgg cgaccttccg ttatcacggc atgctcagtt
     8821 cgttccgcct gcgcccgacg cgcggcctga cggcgcaact ctcctatacc tggtcgaaga
     8881 acatgaccga tgcgacgaac gaccgcgatg ctattgacgt gccgcagatt cgcaccaact
     8941 tccagttgga gcgcgccgtg tcgcgtctcg accgcacaca cgtcttcgtc gccagttacg
     9001 tctatgaagt gccgaccttc tcgtcgggct tcgcctcgac cacgttcggt cgcctcctgc
     9061 tgagcggctg ggaactggcc gggatcacga cggcgcagac ggggctgccc ctgaatcgcg
     9121 tcgtgcaggg ttcggctacc attccggcgc gcggcagccg cccgaacatc atcggcgacc
     9181 cgatgcagaa catccccgtc aatccgagcg gcggcatccc ttacgccttc aacccgctcg
     9241 ccttcagacc gacgaacacg ggcgagatcg gaaattcgcc gcgttcgcct ttacgcttcc
     9301 cgaaccaatt cttcacggat ttgaacgtga cgaagaacct gcgtttcacc gagcggtatc
     9361 gcgtgcagct tcgcgcggag ttcttcaaca tcttcaacca cgcgatcttc aacgatgtct
     9421 tccagaccat tccagatcgc tcgccgactg acccggcatt caacagcatc cagaatctgg
     9481 ctgccgtctc tcagttcgga caattcttct cgacgcgcaa cccgcgcgag attcaactcg
     9541 gcttgaagtt ctacttctaa ggcgagcgtc cgacgcaacc tgagagagcc gccgtagtct
     9601 aaaaactacg gcggctctct cgtctgcaca gcgaaaattt tcggatgccg cgccggatgc
     9661 aaacggcgga ctgcttcaaa gcgtgcggct caattcttct tgggttggct cactttctct
     9721 tccaaacgct tgcgctcctc ctggccgagc ctttgcgccg tggcgaatgc ttgtcgagcg
     9781 tcgccgcgct tgcccgcaga catgtatgcg cgaccgagca gcgcgaaccc ttgcggccac
     9841 tccgggtcga gtttcaccgc gcgttccagc acgcgcacgg cctcagtcgc cgcgccctgt
     9901 ttgagtagcg cctgccccag agtctccagc gcggggcgga agttcggctc aacctccaag
     9961 actttattca gcagcgcgac tgcttcctcc aagcggcctt tctgaaagta gacgttgccg
    10021 agttggtagc gagccagcag catgccgggg ttcagttcca actcctgctc aaactgcgcg
    10081 accgcttcgt ccagatttcc acgcttgagc agggcataac cgagcgcgta atgagcttcg
    10141 ggcagtttcg ggtcggcggc gacggcgcgg cgcaactctt caatcgcgcg ctccgactcg
    10201 cccgcgttgt ctaacgcttc gcccagacgc aggcgcgctt tggccgcacc gccttcgggc
    10261 tgcaatcgaa tcgagcgttc cagatgcggc acggctttcc tgtattcttt gaggcgcagg
    10321 tacgtctcgc cgagcgcggc gagcgaggcc gcatcgttcg ggttgagcgc cacggagatt
    10381 tcgagttcgg cggcggcctc ttcgaa
//
LOCUS       DASWNS010000353         7294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_14442, whole genome shotgun sequence.
ACCESSION   DASWNS010000353 DASWNS010000000
VERSION     DASWNS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14442"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VGB76_12390"
     CDS             complement(<1..337)
                     /locus_tag="VGB76_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0099741.1"
                     /translation="MIIVNRREAAVIKTPHGSEIRPLMDRTTSEIELCSLAEEVLPPG
                     ASVGRHHHLQTEEVYYLLEGRGRMTVAGESREVAAGDAVFIPRGATHTLANTGDAPLR
                     LLLVCGPAYS"
     gene            488..560
                     /locus_tag="VGB76_12395"
     tRNA            488..560
                     /locus_tag="VGB76_12395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Arg,seq:ccg)
     gene            906..1733
                     /locus_tag="VGB76_12400"
     CDS             906..1733
                     /locus_tag="VGB76_12400"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0099742.1"
                     /translation="MKSVAAGVLPARALNVYLSALGSADYHLGRDTRERCRPLLADHL
                     KVPPRSSSYEFYLSQLCRNLVRWSVPLKHAYWRDSSIRYEWVCEQNIRRCLADGRGVI
                     ILQSHFGPWPSTVHEFERHGLRMELTYPLGWPHMSRFDPEARLEFLRRARRILSNNGI
                     VALMGDVGIIGLNQGLGRVVELPFLGMKMPFPLGCARLSALTSSPIVPIFSLRQADGR
                     HFMICTEPIESSRFEGSESERAVQMMACYASRLEEMVKAYPHNASSYLEFAHRARAA"
     gene            1894..2343
                     /locus_tag="VGB76_12405"
     CDS             1894..2343
                     /locus_tag="VGB76_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY0099743.1"
                     /translation="MDAFELLKNDHAKVSSLLEKAEAATDAPTRQQLFTQLKQELDIH
                     AHIEEVILYPALQGAEETSDLTEEAYEEHQEVKDLLSELEATPADSEEWGDILLELKD
                     SVEHHVEEEEGEMFPKARTVLSTEQLNEIGARLSAEKQQQQKAAAAG"
     gene            2530..4224
                     /locus_tag="VGB76_12410"
     CDS             2530..4224
                     /locus_tag="VGB76_12410"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY0099744.1"
                     /translation="MQRFKLLNLALACALLINLAPAHAQTQGTGAAATATPPPAATLS
                     AAALEASKLITAARLSEHLHIVASDAMGGRDTPSPGLDETAKYIATHLTRFGLKPAGD
                     DGTFFQRIALIRRKVDAAKTNATLDGRTFKFGEDFLVSPAIGKASGALVFGGHGWVFK
                     SKNIDAYKGIDVRDKIVVVAAGLPAGVTRADLKGKAGEDWEDPESYARRGGAKGLIII
                     PRTNNLERWWRAVRSGAERGAVEMERFANAEGSGQNLPTIFASAALVDALFAGETVSG
                     ADIFKATQAGETRPAFALSPKKRMTFDVVATVDKMSTQNVVAVLEGRDATLKSEYVAV
                     GAHYDHVGIGLPDASGDALHNGADDDGSGTVALLAMAEALSRGTAATRPARSILFVWH
                     AGEEKGLWGSQYFTKYPTVPLQQIIAQLNIDMIGRSKKEGDTKPENRDLSGANEIYVI
                     GSKMMSTELGELSERVNRAYLNLTFNYKYDDPADPNRFFFRSDHFNYAQRGIPIIFYF
                     DGVHEDYHRPSDTPDKIDYQKMEKITRTVFLTALELANALTRPRVDKKLPAELTGQ"
     gene            4390..4462
                     /locus_tag="VGB76_12415"
     tRNA            4390..4462
                     /locus_tag="VGB76_12415"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4423..4425,aa:Lys,seq:ctt)
     gene            4658..>7294
                     /locus_tag="VGB76_12420"
     CDS             4658..>7294
                     /locus_tag="VGB76_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEY0099745.1"
                     /translation="MADDFKYDVFLSHSSKDKAVARSLAERLREDGLRVWFVDWEVRL
                     GSTRVMREKKIEEGLEHSRMLVLCLSAHASGSEWPQLEATALRFRDPLNKERRFIILR
                     LDATPIKDSLSQFPYINWLPQARAHKYAKVLEACRPQAKSLYVERQGRRKQVAEKTIK
                     LDHRETIWSYAFNSDEKRTLTGSEDHTVRLLDLETGQCLRVLKGHTSRVYSVAWSTDQ
                     RRALSGSQDHTVRLWDVETGRSLHVLEGHKDVVQSVAWSPDQRFALSGSHDHTMRLWD
                     LETGRCLRVFEGHSGWVWTVVWSADQRRALSGGNDETVRLWDLETGHCLRVLSGHTGG
                     LKSLAWSLDERRVLSGAADTTVRLWELETESCLRILEGHTDYVRSVSWSADQCHALTS
                     ADDRDVRLWDMETGRCLRVLKGHTRSVWSIAWSVDQRRAFSGDQGGVVRVWDLSEFVT
                     GTRATEAAATVLPTEPEQVQYTNAKVLLVGDTSAGKTGLAHRLATGEWKPSEASTVGA
                     WSTQWKLNDGGGAPGVEREIWLWDFGGQADQRLIHQLYMDHTSLILLLFNADQEDVLP
                     GLRDWLTALSRCVPPETPQFLVAGRVDTGFKASRGKLQSFARENHLDYHETSALAGTG
                     CAELHAAILSTIPWGDIPIITTEQLFKLIKDEILKLRDEGQVLHTFKELRDLLWHRLP
                     REAQFTDETLRTVVGHLDAPGAVKELDYGTYILLQPEWVNAYAQAVIRTLRSDETELG
                     CLPLRSIAEGKLVYQSMGRDGGIVDMRRLPQPDERVVLGEMERQLLERGLCLRQGDKL
                     VFPSHCGRERPAVVEHPSVFVSYAVKGYLDDIYATLVVKLADSESFKLKELWRDAADF
                     DTLEGGHRMGIKLSRESASGGEI"
BASE COUNT         1491 a         2173 c         2210 g         1420 t
ORIGIN      
        1 ggctgtaggc gggaccgcag acgaggagca ggcgcagggg cgcgtcgccc gtgtttgcca
       61 gagtgtgcgt cgcgccgcgc gggatgaaga cggcatcgcc cgccgcaact tcgcgcgact
      121 ctccggccac cgtcatgcgc ccgcgccctt cgagcaggta gtaaacctcc tccgtctgca
      181 aatgatgatg ccgcccgacg ctcgcgcccg gcggcaacac ttcctccgcc agactgcaca
      241 actcgatctc cgacgtggtg cgatccatga gcgggcgtat ctccgacccg tgcggcgtct
      301 taataacggc ggcctcccgg cgattgacga tgatcataat ctctaatgct ttcgagttcc
      361 tttcccctcc ggccttctct ctaaccacgc gtggtgtagc cccacgcgcc gctccgtgtc
      421 aagcagcagg cgtcactggc acgcgcggcg agtgctatgt tatgttaacg gcctccgtaa
      481 gtcttatgcg cccgtagctc agtggataga gcgcttgcct ccggagcaag aggccgtagg
      541 ttcgactcct accgggcgta tcgttttact aaggaatttg cagtttccgg cgtctcgctc
      601 gcccttcttc ccatcacatg acaacgcatt ttccactttc agaaattgaa gggtgaaagc
      661 cgcgtgcgga cgcgacgaaa gcgagcctgt gcttgaaggg tcgcgcgcgt cgccgtgatc
      721 atatttgact taggagaagg tcaacaatta ctttcagaga cggtagtgac gaaagcgttc
      781 gcccggagta gttcgtattt tccctacgca ttctcaaggg cggttacatc gtggacatta
      841 tttaaggagc cttcatttac atttcacatc tcctcagaaa cctctcgcgc cgtgctttgt
      901 tcgagttgaa gagtgttgcc gccggagttt tgcccgcgcg cgcgctcaac gtctatctct
      961 ccgcgctcgg ctccgccgac tatcacctcg ggcgtgatac gcgcgaacgt tgccgcccgc
     1021 tgctcgccga ccacttgaag gtgccgcccc gctcaagctc ctacgaattc tatctctcgc
     1081 aactctgccg gaatctcgtg cgctggtcag tcccgctcaa acatgcttac tggcgcgaca
     1141 gctcgatcag gtacgagtgg gtctgcgagc agaatatccg tcgttgtctg gccgacggtc
     1201 gcggcgtcat cattttgcag agccacttcg gcccctggcc ttccacggtt cacgagttcg
     1261 agcggcacgg actgcgcatg gagttgacct atcctctcgg ctggcctcac atgtcgcgct
     1321 tcgacccgga ggcgcggctc gaatttttgc gcagggcgcg acggattctg agcaataacg
     1381 gtatcgtcgc gctcatgggc gatgtcggca tcatcggact caaccagggg ctggggcgcg
     1441 tggtggagtt acccttcctc ggcatgaaga tgcctttccc gctcggctgc gcacgcttat
     1501 ccgcgctgac ctcttcccct atagtcccga tcttctctct gcgacaggca gacggcaggc
     1561 acttcatgat ctgcaccgag ccaatcgagt cgagccgttt tgaaggttcg gagtcggagc
     1621 gcgccgtgca gatgatggcc tgctacgcct cgcggctcga agagatggtc aaggcatacc
     1681 cgcacaacgc atccagctat ctggagtttg cgcacagggc gcgagccgct tgagcacgcg
     1741 cgtgtgtagc tcgcgcgtct cgtgcgtcgc gtgcgtcgcg tgccctgctg gcatcgcgct
     1801 tgcctctcaa gctgcgggcg gcctcgcgcg cgttcgatta gcgtgcaagg tctgggtaca
     1861 taacagacac attcaatcaa gggagagtta gagatggacg cttttgaact gttgaaaaac
     1921 gatcacgcca aagtttcttc cctgttggag aaggcggagg cggcgaccga cgctccgacg
     1981 cgccagcagt tattcacgca gttgaaacag gaactcgaca tccacgcgca tatcgaagag
     2041 gtgattctct atcccgcgct ccagggggcg gaggagacga gcgatctgac ggaggaggct
     2101 tacgaagagc atcaggaagt gaaagacctg ctctccgagt tggaagcgac gcccgccgac
     2161 agcgaagagt ggggcgacat cctgttggag ttgaaagata gcgtcgaaca ccacgtggag
     2221 gaagaggaag gcgagatgtt tccgaaggcg cgcacagtgc tgagcacaga gcaactcaac
     2281 gagatcgggg cacgcctgtc agccgaaaag cagcaacagc aaaaagcggc agcggcgggc
     2341 tgagaaagtc cattgcaata ttcttctgcc cggagcgcgg ggacggcttg tagtccccgc
     2401 gctctttatt ttgcacgcgc tctccgggtc gtcgccttcc ggcaaacgct cgccgccgtg
     2461 tttgacatca aagctcggcc agcgtaactc tattcacaga cgaagaaagt caaattatcc
     2521 ggagaagaca tgcaaagatt caaactacta aacctcgcgc tcgcctgcgc tttgttaatc
     2581 aacctcgcgc ccgcgcacgc gcaaactcaa gggacgggcg cagccgcgac agcgacgccg
     2641 cctcccgccg caacgctctc cgccgccgcc ctcgaagcgt cgaaactgat cacggcggcg
     2701 cgtctgagcg aacacctgca catcgtcgcc tcggacgcga tgggggggcg cgatacgcct
     2761 tcgccgggtc tggacgaaac ggcgaagtac atcgccacgc atctcacacg cttcggcctc
     2821 aaaccggcgg gcgacgacgg cacgttcttc caacgcatcg cgctcatccg gcgcaaagtt
     2881 gacgccgcga agacgaacgc cacgctcgac gggcgcacgt tcaaattcgg cgaagacttt
     2941 ctcgtctccc ctgccatcgg caaagcgagc ggcgcgctcg tcttcggcgg ccacgggtgg
     3001 gtcttcaaat caaagaacat agacgcttac aaaggcatag acgtgcgcga caagatcgtc
     3061 gtcgtcgcag ccggactccc ggcgggcgtc acgcgcgcag acctcaaagg caaggccggt
     3121 gaagattggg aagaccctga atcctacgca cgtcgcggcg gcgcgaaagg actcatcatt
     3181 attccgcgca cgaacaacct cgaacgctgg tggcgcgccg tgcgttcggg agcggagcgc
     3241 ggcgcggtcg agatggaacg cttcgccaac gcggagggca gcgggcagaa tttgcccacc
     3301 atcttcgcgt cggcggcgct ggtggacgcg ctctttgcag gcgagactgt gagcggcgca
     3361 gacattttca aagcgacgca ggcgggcgag acgcgcccgg ccttcgcgct ctccccgaag
     3421 aagcgcatga ccttcgatgt cgtggcgacg gtcgataaga tgtccacgca aaacgtcgtc
     3481 gccgtcctcg aaggcagaga cgccacgctc aagtctgagt acgtcgccgt aggcgcgcac
     3541 tacgatcacg tcggcatcgg cttgcccgac gccagcggcg acgcgctcca caacggcgcg
     3601 gacgacgacg gctcgggcac ggtcgcgctg ctcgccatgg ccgaagccct ctcgcgcggc
     3661 acggctgcga cgcgccccgc gcgctcgatc cttttcgtct ggcacgcggg cgaggagaaa
     3721 ggtctgtggg gttcgcagta tttcacgaag tacccgaccg tcccgctcca gcagatcatc
     3781 gcccaactca acatcgacat gatcggtcgg agcaaaaaag agggcgacac gaagcctgaa
     3841 aatcgcgacc tctcaggggc gaacgaaatt tacgtcatcg gctccaagat gatgagcacg
     3901 gaactcgggg agttgagcga acgcgtcaat cgcgcctatc tcaatctcac cttcaattac
     3961 aaatacgacg acccagccga tcccaatcgc ttcttcttcc gcagcgatca tttcaactac
     4021 gcccagcgcg gcatccccat catcttctac ttcgacggcg tccacgaaga ttatcatcgc
     4081 ccctcggaca cgcccgacaa gatcgactac cagaagatgg agaagatcac gcgcacggtc
     4141 ttcctcaccg cgctcgaact cgccaacgcg ctcacgcgcc cgcgcgttga caaaaaactc
     4201 ccggcggaac tcaccggaca gtagcaatac cagggatgaa ggatgagggc tgagggatga
     4261 attaagaaac actcaagcga ttgaatgctt tctctgattc atccctcagc cctccgccct
     4321 catccctttc tccatcccgc tcccgtttct tgacacgccc acactcatca tataatttct
     4381 tcacaacacg ggccgatagc tcagttggta gagcagcaga ctcttaatct gcgggtcgct
     4441 ggttcgagcc cagctcggct catctcagta aaaagggggc acttatcaaa gtgtcccctt
     4501 tctcttttct caataaactt tccagacgtt tttccgctta taatggtcac gctcaaccag
     4561 aacgccggaa ccgggtttga cgctcatcag tgatcaattc tctttcgacc ctttcccgcg
     4621 ccgccacgcg aaggacaggc aacgccagag gtgaagcatg gctgacgatt tcaaatacga
     4681 cgtattcctg agccacagtt caaaggacaa ggcggtcgcg cgctccctcg cggagcggct
     4741 gcgagaggac ggcctgcgtg tgtggttcgt tgattgggag gtcaggctcg gcagcacaag
     4801 ggtcatgcgc gaaaagaaga ttgaggaagg cttggagcac tcccgcatgc tcgtgctctg
     4861 cctgtcggcg cacgcgtccg gctccgagtg gccgcagttg gaggccaccg cgctccgttt
     4921 ccgcgaccct ctcaacaagg agcggcgctt catcatcctg cgcctcgacg ctaccccgat
     4981 caaagactcc ctgtcgcaat tcccttacat caactggcta cctcaagctc gcgcgcataa
     5041 gtatgcgaaa gtcctcgaag cctgccgccc tcaagcaaag tccttatatg tggagcggca
     5101 gggcaggcga aagcaggtcg cagaaaagac catcaaactt gaccacaggg aaactatctg
     5161 gtcttacgca ttcaattcag atgaaaagcg tacacttacc ggctcggagg atcataccgt
     5221 gcggctattg gacttggaga ccggacaatg tctgcgcgtc ctcaaaggtc acacgagccg
     5281 agtctatagc gtggcgtgga gcactgacca acgccgcgcc ctctccggct cgcaggatca
     5341 tactgtgcga ctatgggatg tggagacggg gcgctccctg catgtacttg aagggcacaa
     5401 agacgttgtc cagagcgtgg cgtggagccc cgatcaacgc ttcgcgctct cgggctctca
     5461 tgaccatacc atgcgactat gggacttaga gactggacgc tgcctgcgcg tgtttgaagg
     5521 gcactctggc tgggtctgga ctgtggtgtg gagtgccgac caacgccgcg ctctctctgg
     5581 cggcaatgac gagacggtgc ggctgtggga cttggagacg gggcattgtt tacgagttct
     5641 gagtggccac acgggcggtc tgaagagttt ggcgtggagc ctcgatgaac gccgcgttct
     5701 ctcgggagca gcggacacga ctgtgcggct atgggaattg gagactgaaa gctgcctacg
     5761 tattcttgaa ggtcacacgg actatgttag aagcgtttca tggagtgccg atcagtgcca
     5821 cgccttaacc agtgctgatg atcgcgacgt acggctgtgg gacatggaga cagggcgctg
     5881 cttacgcgtg ctcaaaggcc atactagaag cgtatggagc atagcatgga gtgtcgatca
     5941 acgccgcgct ttctccggcg accaaggcgg cgttgttcga gtgtgggatt tgtcggaatt
     6001 tgttaccggg acaagagcaa cggaagccgc cgcaacggtc ttgcccaccg agccggagca
     6061 ggtgcagtac acgaacgcga aagttttgct ggtgggcgat acgagtgcgg gtaagacggg
     6121 gttggctcac cgtttggcga cgggtgagtg gaagccgagc gaagcctcga cggttggagc
     6181 gtggtctacg cagtggaagt tgaatgatgg gggcggtgcg ccgggggtgg agagggagat
     6241 atggttgtgg gactttgggg ggcaggcgga tcagaggttg attcaccagc tttatatgga
     6301 tcatacgtcg ctgattctgc tgctcttcaa tgcggatcag gaggatgtgc tgccgggtct
     6361 gcgcgattgg ctgacagcgt tgtcgcgttg tgtgccgccg gagacgccgc agttcctcgt
     6421 ggcggggcgc gttgatacag ggttcaaggc gagccggggc aagttgcaaa gtttcgccag
     6481 agagaaccat cttgactatc acgagacgag cgcgctggcg ggcacgggct gtgcggaact
     6541 gcatgcggcg atcctctcca ccattccgtg gggcgacatt ccgatcatca cgaccgagca
     6601 actgttcaag ctcatcaagg acgagattct gaaactgcgt gatgaggggc aggtgttgca
     6661 caccttcaag gaattgcgcg atctgctctg gcaccgcctg ccccgtgagg cgcagtttac
     6721 agacgagact ctgcgcacgg tggtcggcca cctggacgcg cccggcgcgg ttaaggaact
     6781 cgactacggc acttacatcc tgctgcaacc ggagtgggtc aatgcctatg cgcaggccgt
     6841 catacgcacc ttgcgcagcg atgagacgga gttgggttgc ctgcccctgc gcagcatcgc
     6901 ggagggcaaa ctggtttatc aaagcatggg acgcgacggt ggtattgtgg acatgagacg
     6961 cctgccgcag ccggacgagc gcgtggtgct cggcgaaatg gagcggcaac tgctggagcg
     7021 cggcctctgc ctgcggcagg gagacaagct cgtcttcccc agccattgcg ggcgtgaacg
     7081 tccggcggtg gtcgagcacc cgtccgtgtt cgtcagctac gcggtcaaag gttacctcga
     7141 tgatatttat gcgacgctgg tggtgaaact ggcagatagc gagtctttca agctcaagga
     7201 actgtggcgc gatgcggcgg acttcgacac gctggaaggc ggtcatcgca tgggcatcaa
     7261 gctctcgcgg gagtcggcca gcggcggcga gatc
//
LOCUS       DASWNS010000354         4736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_92208, whole genome shotgun sequence.
ACCESSION   DASWNS010000354 DASWNS010000000
VERSION     DASWNS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92208"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..2333
                     /gene="pnp"
                     /locus_tag="VGB76_12425"
     CDS             186..2333
                     /gene="pnp"
                     /locus_tag="VGB76_12425"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY0099746.1"
                     /translation="MAVKKYLKETIKLGGRDLTVETGRVAKQADGSVVIRYGDTMLLV
                     AAVGASSPREGIDFFPLTVEYREHNYAAGRIPGNYFRREGRPGEKEVLTSRLIDRPCR
                     PLFTEGYRNETQVIASVISADAENDPDVIAITGASCALYLSDIPFPTPIAGVRVGLIE
                     GRYIINPTYDEVRESRLNLIVAGTEEAIVMVESGAKEVSEEIMVEALMLAHKEINRLC
                     RWQKELYKALDIQKREVTPPVLNEEMAGEIERNYSEKLRAALDTSGQDKLASYAAVDA
                     LKKEVVESYPEDAPEQRQMAKKLFDKQKEKIFRDDILDKRRRPDGRRFSEIRPITCEV
                     GWLPRVHGSSLFTRGETQAIVTTTLGTKEDEQFMDDLETGEIKRRFLLHYNFPSFSVG
                     EVGRFGSTSRREIGHGALARRALEPVLPEESEFPYSLRIVSDITESNGSSSMATVCGG
                     TLSMMDAGVPIKSSVAGVAMGLVMEGNKYAILSDIAGAEDHYGDMDFKVAGTRDGITA
                     LQMDIKIGGINAMILQEALEQARKGRLHILAAMEQAINAPRPEMSPFAPRIIQIKINP
                     DKIRDVIGPGGKMIRSIVEETGAKIDVEDDGTVSIATADSEAAQAAVDRIRGLTAEAE
                     IGATYVGTVSRIVDFGAFVEIFPGTDGLLHISEIANHRVRDVRDELKEGQQIMVKCIG
                     KEGNKIKLSRKSILRDEEQKAGGNGHEPQGDED"
     gene            2625..3251
                     /locus_tag="VGB76_12430"
     CDS             2625..3251
                     /locus_tag="VGB76_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0099747.1"
                     /translation="MLSVLLIAAVPAKAGTIKFGDVIALATINSQSGRPSVDLRLRSV
                     TQQSGGASSQDKQTASNGNNQQDRKNIQTPSATTPAGSAETSITGTNVAVQQPGSVQV
                     ETIELGDIQGTICDCGEIFTPKGGFPRLPLLALAGIPLLFLGGDSDTPPGDIVIIPPP
                     PPPPPQVPEPATLLLLGSGLAALGAGARRRRAARSNGQSDEVLDVRGA"
     gene            3257..4276
                     /locus_tag="VGB76_12435"
     CDS             3257..4276
                     /locus_tag="VGB76_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099748.1"
                     /translation="MSRAKAMSYVAAPIRSKSLPLACAMLLLVAASLAGSTNVYAQNG
                     RVAPNGRIEVSTTPGGYPLLIDGQAPRTGPDRTSPTVQLYELEPGRHTVEIQFPNNTR
                     WVRTFDIASNKKQCITLNWRPKTITIERPSIVKSPCPYPVNISAPASVREGDIITFSS
                     DVNYAGQSGLNYTWTISPPAARIVSGAGTPTITVDTTGIGRQRVTAILVVDDGSGDQM
                     CRQRAQAVTGVTPVELPPVLPKSFAEFPSISFNEDKANLDNFAVALQAEPGARGYVIV
                     YNGRRSRPGRATQLGERSRQYLVNTRNLDPSRIVVVNGGTRETDSFELWLVPQGATPP
                     QPRPR"
     gene            4470..4643
                     /locus_tag="VGB76_12440"
     CDS             4470..4643
                     /locus_tag="VGB76_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099749.1"
                     /translation="MADGDGGSGSTGVVAVLVIFIIVVVVALFLFRGRLFGGGGTTKV
                     DVNVSAPAAPASK"
BASE COUNT         1020 a         1482 c         1399 g          835 t
ORIGIN      
        1 acttcacggg tgaagttgtc gccgcttttg tttgggaaat ttgccgcccg cccgtcgcgc
       61 ctcacgtagc gcgccgctcg gtcaaagcgg gcgtgtagtt agtctgaaat cgaagcagga
      121 cgcgccggag gccggatgag acggctcgac gctaacagat tggttgtccg aaggagacac
      181 atgttatggc agtgaaaaaa tatttgaaag aaactatcaa actcggcggt cgcgatctga
      241 ccgtcgagac gggacgcgtc gccaaacagg ccgacgggtc ggtcgtcatt cgctacggcg
      301 acacgatgct tctggtcgcc gctgtcggcg cgagcagccc gcgcgagggc atagactttt
      361 tcccgctcac ggtcgaatac cgcgagcata actacgccgc cgggcgcatc cccggcaact
      421 atttccggcg cgaaggccgc cccggcgaaa aggaagtcct gaccagccgc ctcatcgacc
      481 gtccctgccg cccgctcttt accgaaggtt atcgcaacga aacgcaggtt atcgcctctg
      541 tcatctcggc ggacgcggaa aacgaccctg atgtcatcgc catcacgggc gcgtcgtgcg
      601 ccctctatct ctcggatatt ccgttcccga cgccgattgc gggcgtgcgc gtcggcctca
      661 tcgaaggccg ctacatcatc aacccgactt acgacgaagt gcgcgagtcg cgcctgaacc
      721 tgatcgtggc gggcacggaa gaggccatcg tgatggtcga atccggcgcg aaggaagttt
      781 cggaagagat catggtcgaa gccctgatgc tcgcgcacaa ggagatcaat cgtctctgtc
      841 gctggcagaa ggaactctac aaggcgttag acatacagaa gcgcgaagtt acgccgcccg
      901 tgctgaacga agagatggcg ggcgagatcg agcgcaacta ctcggagaaa ctgcgcgccg
      961 cgctcgatac ttcgggacag gacaaactgg cgagctacgc cgccgtcgat gcgctgaaaa
     1021 aagaggtcgt cgaatcctac ccggaggacg cgcccgaaca acgccagatg gcgaagaaac
     1081 ttttcgacaa gcagaaagag aagattttcc gcgacgacat tttagacaaa cgccgtcgcc
     1141 cggacggtcg ccgcttctcc gagattcgcc cgatcacttg cgaagtcggc tggctgccgc
     1201 gcgtccacgg ttcctctctc ttcacgcgcg gcgagacgca ggccatcgtc accacgacgc
     1261 tcggcacgaa agaggacgag cagtttatgg acgacctgga gacgggcgag atcaaacgcc
     1321 gtttcctgct gcactacaat ttcccgtcct tctcggtcgg tgaagtcgga cgcttcggct
     1381 cgacgagtcg ccgcgagata gggcacggcg cgctcgcgcg tcgcgcgctc gaacccgtcc
     1441 tgccggaaga gagcgagttt ccctattcgc tgcgcatcgt ctcggacatc acggagtcga
     1501 acggttcgag ttcgatggcg accgtctgcg gcggcacgct ctcgatgatg gatgcgggcg
     1561 tgccgatcaa gtcgtcggtg gcgggcgtcg cgatgggact cgtgatggaa ggcaacaagt
     1621 acgcgatctt gtcggacatc gcgggcgccg aagaccacta cggcgacatg gacttcaagg
     1681 tcgcgggcac gcgcgacggc atcacagccc tccagatgga catcaagatc ggcggcatca
     1741 acgccatgat cttgcaggag gcgctcgaac aggcgcgcaa aggccgcctg cacatcctcg
     1801 ccgcgatgga acaggccatc aacgcgccgc gccccgagat gtcgcccttc gcgccgcgca
     1861 tcattcagat caagatcaac cccgataaga ttcgcgacgt gatcggaccg ggcggcaaga
     1921 tgatccgctc catcgtcgaa gagacgggcg cgaagatcga cgtggaagac gacggcacag
     1981 tctcgattgc gaccgccgac agcgaagcgg cgcaggccgc cgtcgaccgg attcgcggcc
     2041 tcaccgccga ggccgagatc ggcgccacct acgtgggcac ggtttcgcgc atcgtcgatt
     2101 tcggcgcgtt tgtcgaaatc ttccccggca cggacggtct attgcacatc tccgagatcg
     2161 ccaatcatcg cgtgcgcgac gtgcgcgacg aactcaagga agggcagcag atcatggtca
     2221 agtgcatcgg caaagagggc aacaagatca agctctcgcg caagtccatt ctgcgcgacg
     2281 aggagcagaa ggcagggggc aacggccacg aacctcaggg cgatgaagac taaaccgtct
     2341 gccaccaacc tatgcggagt ttaacttcgg ggcttgcacc acccgtgcaa gcccactttt
     2401 tgttctaata catacgttta ggccggattt attgtctcgc aaaattggaa gagatgcgct
     2461 attgtgtctt tccttttaat gcaaacgacc ccacagcgga atttttttaa gggtttccgc
     2521 cgggagtcgc atcccaaaaa gccattattt atggtagtta cggtgaagcc caaggaggac
     2581 gaccacacac gatgaagcat caagccctac gcacattcgc tctgatgctc agcgtcttac
     2641 tcatagccgc tgtgcccgcc aaggctggca cgatcaagtt cggcgatgtg attgcgctag
     2701 caacaataaa cagccagagt ggccgccctt ccgttgatct acgcctccgc tccgtcacgc
     2761 aacaaagcgg cggcgcaagt tcacaggaca agcagaccgc ctctaacggc aacaatcaac
     2821 aggatcgcaa aaatattcag acgccctctg cgaccacccc tgcgggcagc gcagagacga
     2881 gcatcacggg cacgaacgtc gccgtgcagc aacccggatc ggttcaagtc gagactatcg
     2941 aactcggcga catccaaggg acgatctgcg actgcggcga aatcttcacg cccaagggcg
     3001 gctttccgag actgccgctg ctggctctgg cgggcatccc gctcctcttc ctcggcggcg
     3061 acagcgacac cccgccgggt gacattgtaa ttattccgcc gcccccgccg ccgccgccgc
     3121 aagtgcccga accggccacg ctcctgttgc tcggcagcgg tctggcagcc ctcggcgcag
     3181 gcgcacgccg ccgtcgcgcc gcacgctcca acggccaaag cgatgaagtt ttggatgtga
     3241 ggggggctta aaagttatgt cgagagcaaa agccatgagt tacgtcgccg ctcctatccg
     3301 ctccaagtct ctgccgctcg cctgtgcgat gctgctgctc gtcgcggcga gcctcgcggg
     3361 gagcacgaac gtctacgcgc aaaatggtcg cgtcgcgcca aacgggcgca tcgaagtttc
     3421 gaccacgccg ggcggctacc cgctgctcat cgacgggcag gctccgcgca ccggacctga
     3481 ccgtaccagc ccgaccgtcc agttatacga actcgaaccc ggtcgccaca cggtcgaaat
     3541 ccagttcccg aacaacacgc gctgggtgcg caccttcgac atcgcctcga acaagaagca
     3601 gtgcatcacg ctcaactggc gaccgaagac gatcaccatc gaacgcccgt ccatcgtcaa
     3661 atcgccctgc ccgtatccgg tcaacatctc ggctccggca tccgtgcgcg aaggcgacat
     3721 catcactttc agttccgacg tgaactacgc aggtcaatcc ggtctgaact acacctggac
     3781 gatcagcccg cccgccgcgc gcatcgtgag cggcgcaggc acgccgacca tcaccgtgga
     3841 tacgacgggc atcggacgcc agcgcgtcac ggccatcctc gtcgtggacg acggttcggg
     3901 cgaccagatg tgccgccagc gcgcgcaggc cgtcaccggc gtcacgcccg tcgaactgcc
     3961 gcccgtgctg ccgaagagtt tcgcggaatt tccgtccatc agctttaacg aggacaaggc
     4021 caatctcgac aacttcgcgg tggctttgca ggccgaaccg ggagcgcgcg gctacgtcat
     4081 cgtctataac ggaaggcgca gccgtccggg tcgcgccacg caactcggcg agcgcagccg
     4141 tcaatacctc gtcaacacgc gcaaccttga tccgagccgc atcgtcgtcg tcaacggcgg
     4201 cacgcgtgag acggattcct tcgaactgtg gctcgttccg cagggcgcaa cgcccccgca
     4261 gcccagaccg agataaagca atatgaagtg acaagtgaca agtgacgagt gacaagttaa
     4321 agaacaaatt ttctgtctta acttgtcact cgtcacttgt cacttagaag tggtaactag
     4381 agccacacta ctttccccct tgaagtggaa gcgcactgca aagctcgttc taaatcgtaa
     4441 agagagtaac tccgaaggag gatggtttta tggctgatgg agatggcgga agcggcagca
     4501 ccggagtggt ggctgtgctg gtgattttca taatcgtggt ggtggtcgct ctgttcctgt
     4561 tccgaggcag gttgttcggc ggcggcggga cgacgaaagt tgacgttaac gtgagcgcgc
     4621 cggcagcacc ggccagcaag tagaaaagat tcggtcgagc agcctctcag tgaagagaga
     4681 gacgcgccgt gcgacaggcg ggcgcgtctc tctcttcttt tcatttgcgc cgcgcg
//
LOCUS       DASWNS010000355        19968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_196459, whole genome shotgun sequence.
ACCESSION   DASWNS010000355 DASWNS010000000
VERSION     DASWNS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19968
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196459"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGB76_12445"
     CDS             <1..243
                     /locus_tag="VGB76_12445"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEY0099750.1"
                     /translation="DRAALNVIAPQFDWTGGFSEGLALVKTGGKFGYIDKTGRLTIRP
                     QFDNAHNFNGGLARVWLAGKQGYIDKSGNFVWKPTK"
     gene            complement(245..1150)
                     /locus_tag="VGB76_12450"
     CDS             complement(245..1150)
                     /locus_tag="VGB76_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099751.1"
                     /translation="MEKISYLGLPNCYKLSNDKVEVIVTSDVGPRIVRYNFRGAENLL
                     GEVPEAKLTTELGDFKPWGGHRLWVAPEAKPRSYAPDNSPVGVESAGEREIRLTAPVE
                     TATGIEKELRITLDAGEGSGLSVVHRITNRNLWAIEAALWGLTVMRGGGTAIFPQEPY
                     ISWDDYLLPARPLVLWHYTDLTDARWSIGKKFIRLRTDASASHPQKIGLLNKQGWAAY
                     AHGATLFLKRFAYEESANYPDYGCNCETYTAGDFIEVESLSPLRRLEPGASAEHLERW
                     SLFEGVEAGATEEELEAAIAQLVAG"
     gene            1351..1432
                     /locus_tag="VGB76_12455"
     tRNA            1351..1432
                     /locus_tag="VGB76_12455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1385..1387,aa:Leu,seq:tag)
     gene            1540..2955
                     /gene="tig"
                     /locus_tag="VGB76_12460"
     CDS             1540..2955
                     /gene="tig"
                     /locus_tag="VGB76_12460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY0099752.1"
                     /translation="MKTELVDVSTTRKELKIEIDAADVRAEMERVTERYAAAVNVPGF
                     RKGRAPNSVVRQRYKNEIRGEVVQNLVPNAINEAINESALNVIGEPDIHLENNEALEK
                     LGEQPLSIHAHVEVLPEVALGDYKGLEVARRTRPVTDETVAEMIENLREQSASLQPVE
                     DRGAEAGDTVTVNFTGKYIEPPDAEDIKADDVQVVLGGEGVLPDFTEQLTGTKPDDVK
                     TFRVSYPEDFSSQGLAGKVIDYTATVTGVSRKELPEIDDEWAKTFGEEIDSLQTLRDK
                     LRENLTERARVESEHRLRDEVMGKLLAAHEFDVPESLVKSQARRLLESTVRDMMQRGM
                     DPRNQELDWENLQGMLEAQATEDLRGSILLERIADAEEIEPTDEEINQEIESIAQGAR
                     QSVEQVRAALTKQGGERSIADRLRHRKALDLLIENARVSEEEWREDAPPAAPAPEAAT
                     AQTDETQADGETPSGEAKADD"
     gene            3007..3594
                     /gene="clpP"
                     /locus_tag="VGB76_12465"
     CDS             3007..3594
                     /gene="clpP"
                     /locus_tag="VGB76_12465"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEY0099753.1"
                     /translation="MALVPMVVEQTSRGERAFDIYSRLLKDNIIFISTPIDDQIANLI
                     VAQLIFLEAEDPEKDISLYINSPGGSITAGMAIYDTMQFIRADVSTLCVGQCASMAAL
                     LLAAGAKGKRFALPNSRILIHQPSGGAEGQATDIRIMAEEIIRMRELTSNILAKHSGQ
                     DYEQIERDVERDRIMSAMQAKEYGLIDEVITHREA"
     gene            3792..5036
                     /gene="clpX"
                     /locus_tag="VGB76_12470"
     CDS             3792..5036
                     /gene="clpX"
                     /locus_tag="VGB76_12470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY0099754.1"
                     /translation="MVRRTDDTLRCSFCGKSQNEVKKLIAGPTVYICNECIDICNEII
                     TDDQQAEAVATRPPLPKPSEIKTFLDEYVIGQDETKKRLAVAVYQHYKRIEIARRRSE
                     VEIQKSNILLMGPTGTGKTLLAQTLARMLSVPFTIVDATTLTEAGYVGEDVENIILKL
                     LQAANGDVERAQQGIIYIDEVDKICRKDENPSITRDVSGEGVQQALLKILEGTVANVP
                     PQGGRKHPHQEFFPIDTTNILFICGGAFVGLEKVIERRMRNKSFGFRAEIKDSKERYS
                     EALAAVQPEDLVKYGLIPEFVGRLPVLGVLNELDESALLRILTEPKNALIKQYQKKFE
                     YDNVKLRFTDDALKAIARQAVERKVGARGLMMIMEELLLDAMYTLPSQKRVKEFVITS
                     EMVERRRAVPGEALTGVDDAAA"
     gene            5089..5544
                     /locus_tag="VGB76_12475"
     CDS             5089..5544
                     /locus_tag="VGB76_12475"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEY0099755.1"
                     /translation="MSRKFGAGVFALLLALFCGGAARAQETKSGTQAAPKPEAAEQDA
                     ADLEDPCPGSNTQHELNRCAARARDKADAELNKVYRELIKDTETTERAKLRAAQLAWI
                     RFRDTQCDYESVGNKGGSIYPMVHSFCLARVTNARVKQLQEILREQSEQ"
     gene            5726..8158
                     /gene="lon"
                     /locus_tag="VGB76_12480"
     CDS             5726..8158
                     /gene="lon"
                     /locus_tag="VGB76_12480"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0099756.1"
                     /translation="MEEYSDHTPTDIVTFPMVPIRDVVIFPFTKVAFKIGRPGSIRAL
                     EQALAADRTIFLSTQHDASIDEPVPSEIYGVGTIARILQAQKLENGQTKVVVEGRERA
                     TTIRVVNDNGAFTATVRRAPSALETGQRIEALTQRIHQLIEQHLRLAPEAQTDALQQA
                     MRNQDPSHLTDALASQLHIAVEDKQGLLEIFSVHSRMQRLVEILETEVDKRQLDRTIQ
                     ARVKRQMEKAQKEYYLNEQIKAIHKELGRKDEKAEVEDLRKKIEEAGMTEEAKEKALQ
                     ELHRLEAMPPMSAEGTVSRTYIDWLLSVPWKQKSKEIKDLSKAEEVLNDDHYGLEKIK
                     DRILEHLAVRQLVKNPKGSILCFVGPPGVGKTSLGKSIARATGRKFVRLSLGGVRDEA
                     EIRGHRRTYIGALPGQIIQMMKKAGATNPCILLDEVDKLGADFRGDPSSALLEVLDPE
                     QNNSFQDHYLDVEYDLSKVFFIATANVLHTIPPALQDRLEILRLSGYTEREKLEIARR
                     HLVAKQAEGSGLKAEQVEFTDEGLLEIIRSYTREAGVRNLEREIGSCCRKVARKLVGE
                     GKGEEFREIVTPERVREMLGPIRFRQQQAAAESEIGLALGLAWTEVGGEVLQIEATLT
                     NGRGGVQLTGKLGEVMQESAQAALTCIKARSERIGVSLDHIRKRDLHVHIPEGAIPKD
                     GPSAGVTLATAMASALTRVPVRKDVAMTGEITLRGKVLPVGGIKEKMLAAHRVGVTTL
                     ILPKENEKDLAEVPEEVLAAISVHFVETIDEVLTLALEDACPTDGAADDTAQPPIWST
                     EAPTQSTPTMAD"
     gene            8301..8783
                     /locus_tag="VGB76_12485"
     CDS             8301..8783
                     /locus_tag="VGB76_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099757.1"
                     /translation="MSKHEDAALILKLYDLRREETMRKARTWLAGFNPESAQEIFEAG
                     SGENNAYLRMVMSYWDMAASLVNNGAIDEKMFNDANGEHFFYYAKFEPFIEELRARFG
                     PQMWQHLEELVMRTPDARERLAHLREMSKKMAAMRAVASNKGQADEQPTQTASGATSK
                     "
     gene            8924..9526
                     /gene="yihA"
                     /locus_tag="VGB76_12490"
     CDS             8924..9526
                     /gene="yihA"
                     /locus_tag="VGB76_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY0099758.1"
                     /translation="MKITSAEFVKSSFSEQDWPRTKLPEVAFLGRSNVGKSSLINSLL
                     GVKGLARTSSTPGRTQALNFFLINRRFHFVDLPGYGYARVPRAIRESWGEIVTSYLAK
                     RESLVLLIQIVDSRHEPTKLDLQLREWLTAYAKPHLTVATKSDKLSQNVLGKNLRRAR
                     EVLGALGSGEVISYSATTGHGRERVWRVIEEALIVSQSPS"
     gene            9885..11309
                     /gene="rho"
                     /locus_tag="VGB76_12495"
     CDS             9885..11309
                     /gene="rho"
                     /locus_tag="VGB76_12495"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY0099759.1"
                     /translation="MSQRTPRTRTSSSSSSSASTANGNSGENGNRTTAAAAVVDSPPE
                     DAADKDFIPSGSGDAFNLATLKEMSISKLTQIAKDMDVPGATGMRKQELIFKVLAAQT
                     EKSGLIFSEGVLETLPDGFGFLRAPEYNYLPGPDDIYVSPSQIRKFDLRTGDTVSGQI
                     RPPKEGERYFALIKVEAINFEAPGETRDKVFFDNLTPLYPDERLMLETDHDTLAARVI
                     DLVTPIGKGQRGLIVAPPRTGKTMMLQTIANSISKNHPEVSLIVLLIDERPEEVTDMQ
                     RSVNGEVISSTFDEPPTRHVQVADMVIEKAKRLVEHKRDVVILLDSLTRLARAHNAVV
                     PPSGKILSGGIDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIFEEFK
                     GTGNCEIHLDRRLVDKRLFPAIDIQRSGTRKEELLISKEDLARIWVMRRVLNPLSPVE
                     QMEVVLERLGKTKSNAEFLASMQS"
     gene            11546..12982
                     /locus_tag="VGB76_12500"
     CDS             11546..12982
                     /locus_tag="VGB76_12500"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEY0099760.1"
                     /translation="MLRVAILTSEAVPFAKTGGLGDVAGALPKALIGTGEVDASLCMP
                     LYEQTDRALLKELLFDNLEVEWTGGNRRVRVFLSEDAIRAPAFLIDAPEYFARPNVYG
                     YRDDHTRYAFFCRAALALLKRLGRPPDIVHGNDWPCGFALAELRARRAYDDFYRQTRT
                     LFSIHNLAYQGAFDSRDLGYLGFGWGEVHDAFLLEGAASALKAGLMLSDALSTVSRRY
                     AEEIQTSAQGYGLDWLLRMRRERLIGVTNGVDYEAWNPATDPHIAAHYSADDLSGKRE
                     CKLDLLRRFSLPEELDRPVIAIISRLVAQKGYDLIREAAPAILETGAFFIALGAGASE
                     YEDFLQSFHDHAPHRVGIYKGYAGEPLAHQIEAGADLFLMPSLYEPCGLNQMYSMRYG
                     TVPVVRATGGLDDTVENFDRARGTGNGFKFGEYSARAMLGSIYEALYAYADKDSWRRI
                     QLNGMRADNSWAAAARKYVEVYRAIARM"
     gene            13124..13483
                     /gene="trxA"
                     /locus_tag="VGB76_12505"
     CDS             13124..13483
                     /gene="trxA"
                     /locus_tag="VGB76_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0099761.1"
                     /translation="MSESEFVKDVSDSNFEAEVLQSDRPVLVDFWAAWCAPCRMIAPT
                     VAQVAEKYSDTARVVKLNVDENPNVPQKYGIKGIPTLIMFKNGKEEERVVGATTKEAI
                     SRMIDKHVNASSGTANA"
     gene            13629..14063
                     /locus_tag="VGB76_12510"
     CDS             13629..14063
                     /locus_tag="VGB76_12510"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY0099762.1"
                     /translation="MKQTINSRAKNVGSRSAKSNKRRLLEERLAAAKRLRAVEDARFR
                     ARQAQGKLRRFVSANFRKQEVIDALALRRGECNRCGACCEILFKCPFLKKHEDGTSTC
                     GIYEDRPNQCRLFPIERRDLDEVRGQCSFYFIEKPIKLERAS"
     gene            14153..15469
                     /locus_tag="VGB76_12515"
     CDS             14153..15469
                     /locus_tag="VGB76_12515"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY0099763.1"
                     /translation="MSKVIVVIGAQWGDEGKGKIVDLLAERFDIVARYQGGHNAGHSV
                     YVGERAFVLHLIPSGIIHPGKTCVLGNGMVIDPKAFFEEADRLAAQGLEVTPERVLVS
                     SRAHLILPYHRALDHTSEERLGNERVGTTLRGIGPAYEDKAGRRGIRVADALVPAVLR
                     SRIERNLEDANRIIEAYGGQALDVEEVFTETSRLTERLAPFIADTTHFMNRAARDGRT
                     ILIEGAQATLLDVDHGTYPFVTSSNTTAGGASAGTGLAPHRITGVLGIVRTYTTRVGE
                     GPFPTEMLEGEAELGQLIRERGREYGASTGRPRRCGWFDAFATRYAAEINGFTSVALT
                     KLDVLDTLEEIKVCTGYLLDGRAVESFPAVSQDLRRIEPVYETLPGWNASTEGITEIE
                     KLPPNARAYVEFLSRAIDVEIGLVSTGPERDQTILCRGSALDAWLK"
     gene            15617..15689
                     /locus_tag="VGB76_12520"
     tRNA            15617..15689
                     /locus_tag="VGB76_12520"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15650..15652,aa:Lys,seq:ttt)
     gene            complement(16341..16520)
                     /locus_tag="VGB76_12525"
     CDS             complement(16341..16520)
                     /locus_tag="VGB76_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099764.1"
                     /translation="MLITITLFIAFAAIGWLATRIYKMQMEKRLGRKIEGEHELTSIT
                     SWMEASKDEKPPDER"
     gene            complement(16630..17121)
                     /locus_tag="VGB76_12530"
     CDS             complement(16630..17121)
                     /locus_tag="VGB76_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0099765.1"
                     /translation="MKKLLSSLLLLIWTFAVYAPALAQTAPKNDAGQEAVAAVAHKLF
                     EAMRAKNAEALRALFMPEGQLVAVDKPRTGEGLSKTRVFTADAFAKMISEAKAAEFIE
                     RMPRPEVKIFGDMALVFGRYTFHVGDKFSHCGTNSFHLVRTADGWKIANAASTLEFNC
                     EQK"
     gene            complement(17136..18890)
                     /locus_tag="VGB76_12535"
     CDS             complement(17136..18890)
                     /locus_tag="VGB76_12535"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0099766.1"
                     /translation="MRSISALCCLFLFAVATAAQHKPVDFAELEKTVAAELRENNTPG
                     AAVVVISGERVLFMKGFGTTSAEGGAPVTPGTLFRRRPSRRDQFAAIVDGDRSYRLTL
                     KFSEGRKMKKRLVLSILLTLLASVGQAATPAVNTTAFVGVNVIPMDRERVLSNQTVIV
                     RDGVIVEIGDAKKVRVPGDAQRIDGRGKFLIPGLTDMHVHLFTDDEFPDALAEDEFKI
                     MLAQGVTTIRLMNGTPEQLVLRARSAKGEIVAPTIYAASPQFIGRKSNHAYVVTNEAE
                     ARAAVRKAKTDGYDYLKMTTFLKPEVYEAIIEEAARQNIRVVGHADSRTVGLPRALKA
                     RQQIEHLDSYLEALLPENSPVKGSVSDLYIYQPKNWESLDVLDEKRIPEIARATVQAN
                     PFSVPTLHLFKSTFGIGRTEESIRLQPDIRFYPQKTVDLWVGYNKKYQAQWASPERRA
                     RYVSIRNKLVKAIHDAGGKILAGSDTPEWLLLYGYTLHRELRALNEAGLSPYATLAAA
                     TRNPAEFFGTLDKTGTIERGKRADLVLLEANPLAGIANTERRAGVMLKGRWFAQAELD
                     KTLDEIAPRFQHALEEKH"
     gene            19273..19884
                     /locus_tag="VGB76_12540"
     CDS             19273..19884
                     /locus_tag="VGB76_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEY0099767.1"
                     /translation="MNVIQVLGLALGAAWTSGLNLYATVAVLGLLEHYKLAHLPGGLH
                     VLDNWLIIGVALSLYVVEFFADKIPYVDTVWDALHTFIRVPAGAALAAAATSNVSPTV
                     QVIALLLGGGLALSTHGTKATVRAGANLSPEPLSNWSLSLLEDVLAIGGVALAVVSPV
                     GILVVILIFLLLLVWILPKVVRRLRRMLKAAGDFFRGRTMETR"
BASE COUNT         4281 a         5920 c         5906 g         3861 t
ORIGIN      
        1 gaccgtgccg cgctcaacgt catcgcgccg cagttcgatt ggacgggcgg cttcagcgaa
       61 ggactcgcgc tcgtcaagac gggcggcaag ttcggctaca tagataagac cggacggctc
      121 acgatccgcc cgcagttcga caacgcgcac aacttcaacg gcggcctcgc gcgcgtctgg
      181 ctcgcaggca aacaaggtta tatagacaag agcgggaact tcgtgtggaa gcctacgaag
      241 tagtttagcc cgcgacgagt tgagcgatag ccgcttccag ttcctcttcc gtcgcgcctg
      301 cttccacacc ttcgaataac gaccagcgtt ccagatgctc ggctgacgcg ccgggttcca
      361 gacgccggag cggcgagagc gattcgactt cgataaaatc gcccgccgtg taagtctcgc
      421 aattgcatcc gtagtcgggg tagttcgcgc tttcttcgta agcgaaacgc ttgaggaaga
      481 gcgtcgcgcc gtgtgcgtag gccgcccagc cctgcttgtt gagcaggccg atcttctgcg
      541 ggtggctcgc gctcgcgtcg gttcgcaggc gtatgaactt tttgccgatt gaccagcgcg
      601 cgtccgtcag gtctgtgtaa tgccagagga cgagcgggcg tgcggggagc aggtagtcgt
      661 cccaggagat gtaaggctct tgcggaaaga tggccgtgcc gccgccgcgc atcacggtga
      721 gcccccagag cgcggcctct atcgcccaga gatttcgatt cgtgatccgg tggacgacgg
      781 agaggccgct gccctcgccc gcgtcgagcg tgatgcgcaa ctccttctcg atgcccgtcg
      841 ccgtctccac aggcgcggtc agacgaatct cacgctcgcc cgcagactcg acgccgaccg
      901 gagaattatc cggcgcgtaa ctgcgcggct tcgcttcggg cgcaacccac aggcgatgcc
      961 cgccccaggg tttgaagtcg ccgagttcgg tcgtgagctt tgcctccggc acctcgccga
     1021 gcaggttctc cgcaccgcga aaattgtaac gcacgatgcg cggccccacg tcgctcgtga
     1081 cgatcacttc aactttgtcg ttcgacaact tgtaacagtt cggcaacccg agataagaaa
     1141 ttttttccac tttcaagctc cttgcacggc tatccggcga cgttcatctt ttgctccaga
     1201 gtgtaaaggc ttgagcctcc cgcagacaac agttgtaaat ctcgctcgct agacattcgc
     1261 gccccgataa tttaacttta tcagcttaca agtaccggca ttttcgacgc gggtttaagg
     1321 tgcaacagga acctgtccgg cgagagtcac gcgaaagtgg cgtaattggc agccgcgcca
     1381 gacttaggat ctggtcccgt aaggggtggg ggttcgagtc ccccctttcg catcattgtc
     1441 tggaaagatt ttgcacaaag catttctata atttcaacag gaaggatgcc cgcacggctg
     1501 agccgcacac gagcggcgac gagcgcgggc gttatgttta tgaagacaga attggttgac
     1561 gtttccacca cccggaagga actcaagatc gagattgacg cggcggacgt gcgcgccgaa
     1621 atggagcgcg ttaccgaacg ctacgcggcg gcggtcaacg ttcccggctt tcgcaaaggg
     1681 cgcgcgccga actccgtcgt gcgccagcgt tacaagaatg agattcgcgg cgaggtcgtg
     1741 cagaacctcg tgcccaacgc catcaacgaa gcgatcaacg aaagcgcgct caacgtcatc
     1801 ggcgagccgg acattcatct ggaaaacaac gaggcgttgg agaaactcgg cgagcagccg
     1861 ctctccatcc acgcccatgt cgaggtgctg cccgaagtcg cgctcggcga ttacaagggc
     1921 ttggaagtcg cgcgccgcac gcgtcctgtc acggacgaga cggtcgcgga gatgatcgag
     1981 aacctgcgcg agcagtcggc ctcgctgcaa cccgtcgaag atcgcggggc ggaagcgggc
     2041 gacacggtga cggtcaactt cactggcaag tacatcgagc cgcccgatgc ggaagacatc
     2101 aaagccgacg acgttcaggt ggtgctcggc ggcgaaggcg tgctgccgga tttcacggag
     2161 caactgacgg gcacgaagcc cgacgacgtg aagacttttc gcgtcagcta ccctgaggat
     2221 ttttcttcgc aggggttggc cgggaaagtg atcgactaca cggcgaccgt cacgggcgtg
     2281 agccgcaagg agttgcccga aatcgacgac gagtgggcga aaactttcgg cgaagagatc
     2341 gactcgctcc agactctgcg cgacaaactg cgcgagaatt taacggagcg cgcgcgcgtc
     2401 gagtcggaac accgtctgcg cgacgaggtg atggggaaat tgctcgccgc gcacgagttc
     2461 gacgtgccgg aatcgctcgt caagagtcag gcgcgcaggc ttctcgaatc caccgtgcgc
     2521 gacatgatgc agcgcggcat ggacccgcgc aatcaggagc tcgactggga gaacttgcag
     2581 ggcatgttgg aggcgcaggc gaccgaagat ttgcgcggct cgatcctgct cgaacgcatc
     2641 gccgatgcgg aagagatcga gccgacggat gaagagatca accaggagat cgagtcgatc
     2701 gcacagggcg cgcgtcaatc ggtagagcag gtgcgtgctg ccttgacaaa gcagggaggc
     2761 gaacgtagca tcgccgacag attgcgccat cgtaaggcgc ttgatcttct catcgagaat
     2821 gcgcgcgtga gtgaagagga atggcgcgaa gatgcgccgc ccgccgcccc cgcgcccgaa
     2881 gctgcgacgg cgcagacgga tgagacgcag gcggacgggg agacgccgtc cggtgaagcg
     2941 aaagctgacg actaaagagt cggaagttaa ctttaacttg aacagagatt ttggagtttt
     3001 aagtttatgg ctttagtccc gatggtagtc gagcaaacgt cgcgcggcga acgcgccttc
     3061 gacatttact cgcgcctttt gaaagacaac atcatcttca tctccacgcc gattgatgat
     3121 cagatcgcga acctcatcgt cgcccaactg atctttctcg aagcggaaga cccggagaaa
     3181 gacatctcgc tctacatcaa ttcgccgggc ggctcgatca cagccggcat ggcgatctac
     3241 gacacgatgc agttcatccg tgcagacgtg tccacgctct gcgtcggcca gtgtgcttcg
     3301 atggccgcgc tgctgctcgc ggcgggcgcg aaaggcaaac gcttcgcgct gcccaattcg
     3361 cgcatcctga tccaccagcc ttcgggcggc gcggaaggcc aggccaccga cattcgcatc
     3421 atggccgaag agatcatccg catgcgcgag ctgacctcaa acattctcgc caagcactcc
     3481 ggtcaggatt acgaacagat cgagcgcgac gtggaacgcg accgcatcat gtccgcgatg
     3541 caggccaaag agtacggact cattgacgaa gtgatcacgc accgcgaagc ataaattttt
     3601 accggcgcgt ctcatcgaaa gattcagacg cgccggtcgt tttatgctac agtagttccc
     3661 cggcagtcga aacgcgtccc cacgcgccac cgcttgcggg cacaagttaa acgcacagcc
     3721 cgcctgttgt aagccacttg acaagctgaa ctggattaga aactcgaaac cacggagtcc
     3781 cctataagcc tatggtacgt cgaaccgatg acaccttgcg ttgttcgttc tgcggcaaat
     3841 cgcagaacga agtgaagaag ctcattgccg gtcctactgt ctacatctgc aatgaatgta
     3901 tagacatctg caacgagatc atcaccgacg accagcaggc cgaagccgtc gccacgcgtc
     3961 cgccgctccc gaagccttcc gagatcaaga cctttctcga cgagtacgtg atcgggcagg
     4021 acgagacgaa gaagcgtctc gccgtcgccg tctatcaaca ctacaagcgc atcgagatcg
     4081 cgcgccgtcg ctcggaagtc gagatccaga agtcgaacat cctcttgatg ggaccgaccg
     4141 gcacgggcaa gacgctcctc gcgcagacgc tcgcgcgcat gctctccgtt cccttcacca
     4201 tcgtcgatgc gacgactctg acggaagcgg gctacgtcgg cgaagacgtg gaaaacatca
     4261 tcctcaaact cttgcaggcc gccaacggcg acgtggaacg cgcgcagcag ggcatcatct
     4321 atattgacga agttgacaag atttgccgca aggacgagaa cccctcgatc acgcgcgacg
     4381 tgtcgggcga gggcgtgcag caggctctgt tgaagattct ggaaggcacg gtcgccaacg
     4441 tgccgccgca gggcggacgc aagcacccgc atcaggagtt cttcccgatt gacacgacga
     4501 atattctctt catctgcggc ggcgcgttcg tcggtttgga gaaagtgatc gagcggcgga
     4561 tgcgcaacaa gtcgttcggc ttccgcgccg agatcaaaga cagcaaggag cgttattcgg
     4621 aagcgctcgc ggcggtgcag ccggaagatc tcgtcaaata cggtttgatc ccggagttcg
     4681 tcggacgcct gcccgtgctc ggcgtcttga acgaactgga cgaaagcgcg ctcctgcgta
     4741 ttctcaccga accgaagaac gcgctcatca agcagtacca gaaaaaattc gagtacgaca
     4801 acgtcaagct gcgcttcacg gacgacgcgc tcaaggccat cgcgcggcag gcggtcgaac
     4861 gtaaagtcgg cgcgcgcggc ctgatgatga tcatggaaga gttgctcctc gacgcgatgt
     4921 acacgctgcc ctcgcagaag cgcgtcaagg agttcgtcat tacgagcgag atggtcgaac
     4981 gccgtcgcgc tgtgccgggc gaagcgctga cgggcgtgga cgacgccgcc gcttaaagag
     5041 ttcgcgccgg agttcaattc tctcccacag cgaaaggaag cttcatcgat gtcgagaaaa
     5101 ttcggagcag gagtgttcgc actcctgctc gcactttttt gtgggggagc ggcgcgtgcg
     5161 caggagacga agagcggcac acaagctgcg ccgaagccgg aggcagccga acaggatgcc
     5221 gccgaccttg aagacccctg ccccggttcc aacacgcagc acgagttgaa ccgttgcgcc
     5281 gcacgcgcgc gcgacaaggc cgacgcggaa ctgaacaaag tctaccgcga gttgatcaaa
     5341 gacacggaga cgacggagcg cgccaaactc cgcgcggcgc aactcgcgtg gatcaggttt
     5401 cgcgatacgc agtgcgacta cgaaagcgtc ggcaacaaag gcggctcgat ctatccgatg
     5461 gtgcacagct tctgtctcgc cagagtgacg aacgccagag tcaaacaact acaggagatt
     5521 ttacgggagc agtcggagca gtaattgatg gcgtcacggt tttggaaatc ccgtctgccg
     5581 caagggcagt ggcgattttg aggggtcagt ggcgtcgtcc ggcaaaatgc tttacaatta
     5641 agtttagttc gcaacagggg cgagcggcat caggcggctc gcccttcacg ttatgtagtt
     5701 ttccggcgaa agaagcagca gttttatgga agaatattca gaccacactc ccacagacat
     5761 cgtgacgttt ccgatggtgc ccatccgtga tgtcgtgatc tttccgttta cgaaagtcgc
     5821 gttcaagatc gggcgtccgg gttcgattcg cgcgctcgaa caagctctgg ccgcagaccg
     5881 cacgatcttc ctctccacgc agcacgatgc ctcgattgat gaacccgtgc cttcggagat
     5941 ttacggcgtc gggacgattg cgcgcatcct gcaagcgcag aaacttgaga acgggcagac
     6001 gaaagtggtc gtcgaagggc gcgaacgcgc gacgacgatc cgcgtcgtga acgacaacgg
     6061 agcgttcacg gcgaccgtcc gccgcgcgcc ctccgcgctc gaaaccggcc agcgcatcga
     6121 agccttgacg cagcgcatac accaactcat cgagcaacac ctgcgcctcg cgcccgaagc
     6181 gcagaccgac gcgctgcaac aggccatgcg caaccaagac ccctcgcacc tgaccgacgc
     6241 gctcgcctcg caactccaca tcgcggtcga ggataagcag ggacttctcg aaatcttctc
     6301 cgtccacagt cgcatgcagc gtctcgtcga gatccttgag acggaagttg acaagcggca
     6361 gcttgatcgc acgattcagg cgcgcgtcaa acgccagatg gagaaggcgc aaaaagagta
     6421 ctacctgaac gaacagatca aggcgattca caaagaactc ggacgcaagg acgagaaggc
     6481 cgaggtcgaa gacttgcgca agaagatcga agaggcgggc atgaccgaag aggcgaaaga
     6541 gaaagccttg caggaactac accgtctcga agcgatgccg ccgatgtcag ccgaaggcac
     6601 cgtctcgcgc acttacatcg actggctcct gagcgtgccc tggaaacaga agtcgaaaga
     6661 gattaaagac ctctcgaaag ccgaagaggt tttaaacgac gaccactacg gtcttgagaa
     6721 gatcaaggat cgcatcctcg aacacctcgc cgtgcgtcaa ctcgtcaaga acccgaaggg
     6781 atcgattctc tgtttcgtcg gcccgccggg agtcggcaag acctcgctcg gcaaatccat
     6841 cgcacgcgcg acgggcagaa agtttgtgcg tctgagcctc ggcggcgtgc gcgacgaagc
     6901 ggagatacgc ggccaccgtc gcacctacat cggcgcgctc cccggccaga tcatccagat
     6961 gatgaagaag gcgggcgcga ccaacccctg catccttttg gacgaagtgg acaaactcgg
     7021 cgcggatttt cgcggcgacc cgtcttccgc tctcttggaa gtgctcgacc ccgagcagaa
     7081 caattcgttt caggatcatt atctcgacgt tgaatacgat ctctccaaag tcttcttcat
     7141 cgcgacggcg aacgtgctcc acacgatccc gcccgcgctg caagaccgtc tggaaatcct
     7201 ccgcctctcc ggttacaccg agcgcgagaa actggagatc gcgcgtcgcc atctcgtcgc
     7261 caagcaggcg gaaggcagcg gcttgaaggc cgagcaggtc gagttcacgg acgaaggttt
     7321 gctggagatc atccgttcct acacgcgcga agcgggcgtc cgtaatttgg agcgcgagat
     7381 cggttcgtgc tgccgcaagg tggcgcgcaa actcgtgggc gagggcaagg gcgaagagtt
     7441 cagagagatc gtcacgccgg agcgcgtgcg cgagatgctc ggacccatcc gtttccgcca
     7501 gcaacaggct gcggctgaaa gcgagatcgg cctcgcgctc ggcctcgcct ggactgaggt
     7561 tggcggcgaa gtcttgcaga tcgaagcgac gctcacgaac ggtcgcggcg gcgtgcaact
     7621 cacgggcaaa ctcggcgagg tgatgcagga gtcggcgcag gccgcgctca cctgtatcaa
     7681 ggcgcgaagc gagcgcatcg gcgtcagtct cgatcacatt cgcaaacgcg acctgcacgt
     7741 gcatattccc gaaggcgcga tcccgaagga cggcccctct gcgggcgtca cgctggcgac
     7801 tgcgatggct tccgccctga cgcgcgttcc cgtccgtaaa gacgtggcga tgacgggcga
     7861 gatcaccctg cgcggcaagg tgctgcccgt cggcggcatc aaagagaaga tgctggccgc
     7921 gcaccgcgtc ggcgtcacga ctctgattct gccgaaggag aacgagaaag acctcgcgga
     7981 agtgcctgaa gaagtgctcg cagccattag cgtccatttc gtcgagacga ttgacgaggt
     8041 gctcacgctt gcgctcgaag acgcgtgccc gaccgacggc gcggcagacg acacagcgca
     8101 gccgcccatc tggtcaaccg aagcgcccac gcaaagcacg ccgacaatgg cagactaaaa
     8161 taagggatga aggatgaagg atgagggatg aatcataaaa agcattcaac gcaatgagtg
     8221 ctttttctga ttcatccctc atccttcatc cctcatccct tctctctttc cccgccagaa
     8281 attcaaggag aaaattccct atgagcaaac acgaagatgc agccttgatt ttgaagctct
     8341 acgacctgcg ccgtgaagag acgatgcgca aggcgcgcac ctggctggcc ggtttcaatc
     8401 ccgagagcgc gcaagagata tttgaggcag gcagcggcga gaacaacgca tacctgcgca
     8461 tggtgatgtc ctactgggat atggccgcct cgctcgtcaa taacggcgcg attgacgaga
     8521 agatgttcaa cgacgccaac ggcgaacact ttttctacta cgcgaagttt gagccgttca
     8581 tcgaagaact tcgcgcacgc ttcggcccgc agatgtggca acacctcgaa gaactcgtca
     8641 tgcgcacgcc cgacgccagg gaacgactcg cacacttgcg cgagatgtcg aagaaaatgg
     8701 ccgccatgcg cgcggtggcg tcgaacaagg gacaggccga cgagcagccg acgcagacgg
     8761 cctccggcgc aacgtctaaa taacagtgac gagtgacgag ataaagccgt gaatcgtaaa
     8821 tcgtgaatag agaaaacctg tcttcatcta ttcgcgattc acgattcacg atttacgatt
     8881 ctcttgtctt cgctcgtcac tcgtcacccg tcaccgcttt ctcatgaaaa ttacgagcgc
     8941 ggaattcgtc aagagttcgt ttagcgagca ggactggccg cgcaccaagt tgccggaggt
     9001 ggcgtttctc ggtcgctcga atgtcgggaa gtcgagcctc atcaactcgc tgctaggcgt
     9061 caagggactg gcgcgcacct cttcgacgcc gggacggacg caagccctca atttcttcct
     9121 catcaatcgc cgcttccatt tcgtagacct gccgggctat ggctacgcgc gtgtgccgcg
     9181 cgctatacgc gagtcgtggg gcgaaatcgt gacaagttac cttgccaagc gcgagtcgct
     9241 tgtgttacta attcaaatcg tagattcgcg ccacgaaccc actaaattgg acttgcaact
     9301 tcgtgaatgg cttacagctt atgcgaagcc tcatctaacg gttgcgacaa agtccgataa
     9361 gctctcgcaa aatgtgttag gcaaaaatct caggcgcgcg cgggaagttt taggcgcgct
     9421 ggggagtggc gaagtgattt cttactccgc cacgacggga cacggacgcg agcgcgtctg
     9481 gcgcgttatc gaagaggcct taatcgtctc acaatccccc agttgaaaag tcgaagcgtc
     9541 gctgtgaggc gaccccgtgg cctcttgacg gcatctaacc tttcggttaa aactgaggct
     9601 cacacaaaat aattcgttga actttttact ggatgcgaga cgtttgcagc aattcgccaa
     9661 tcacctcgta tacataaccg ccggaaacat cgccgctcct gcgccgagtc gaatcgcttc
     9721 gcgcacctga ggcatcattc ccgacatcat ttaaggattt aattcatacg cggcagagag
     9781 tccgccagta attcaatctc aacgacagca acatgacaag agttggacac aactcttggt
     9841 tagaaatggc agctaagcaa cgctgcctca cagagaaaag agaaatgtca caacgtacac
     9901 cacgcacccg aaccagcagt agcagcagca gtagcgccag cacggccaac ggcaacagcg
     9961 gcgagaacgg caaccggacg accgctgccg ccgccgtcgt cgatagcccg cccgaagatg
    10021 cagccgacaa agatttcatc ccgtcaggca gcggcgacgc cttcaatctg gcgacgctca
    10081 aggagatgtc catctcgaag ttgacgcaga tcgccaagga catggacgtg cccggcgcga
    10141 ccggcatgcg caagcaggaa ctcatcttca aagtcctcgc ggcgcagacc gaaaagagcg
    10201 gactcatctt ctccgagggt gtcctcgaaa ctttgcccga cggcttcggc ttcctccgcg
    10261 cgcccgaata caactatctg cccggccccg acgacatcta cgtcagccct tcgcagatac
    10321 gcaaattcga tctgcgcacg ggcgacaccg tctccggcca gattcgcccg cccaaagagg
    10381 gcgagcgtta cttcgcgctc atcaaagtgg aggccatcaa cttcgaagcc cccggcgaga
    10441 cgcgcgacaa agtcttcttc gacaatttga cgccgctcta cccggacgag cgcctgatgc
    10501 tggaaaccga ccacgacacg ctcgcggcgc gcgtcatcga tctcgtcacg cccatcggca
    10561 aaggccagcg cggcctcatc gtcgcgccgc cgcgcacggg caagacgatg atgctccaga
    10621 ccatcgccaa ctcgatctcg aagaaccacc ccgaagtctc gctcatcgtg ctcctgattg
    10681 acgagcgacc ggaagaagtt acggacatgc agcgttctgt gaatggcgaa gtcatctctt
    10741 cgaccttcga cgaaccgccg acacgccacg tgcaggtcgc ggacatggtc atcgagaagg
    10801 ccaagcgact cgtcgagcat aaacgcgacg tggtcatcct gctcgactcc ctgacgcgtc
    10861 tggcgcgcgc gcacaacgcc gtcgtgccgc cttcgggcaa gattctctcc ggcggtatcg
    10921 acgccaacgc gctgcaacgt cccaagcgtt tcttcggcgc ggcgcgcaac atcgaagagg
    10981 gcggaagttt aaccatcatc gccaccgctt tgatcgatac cggctcgcgc atggacgacg
    11041 tgatcttcga agagttcaaa ggcacgggca actgcgaaat ccatctcgac cgtcgcctcg
    11101 tagataaacg cctgttcccc gccatcgata ttcagcgctc cggcacgcgc aaagaggaac
    11161 tcctcatcag caaggaagac ctcgcgcgca tctgggtgat gcgccgggtg ctaaaccccc
    11221 tgtcccccgt cgagcagatg gaagtcgtgc tggaacgcct gggtaaaacc aagtcgaacg
    11281 ccgaattcct cgcttcgatg caaagctaag agaacagtga ccagtgacaa gtgacgtgtg
    11341 acgagagaag aaagtgaatc gtgaatcgta gatcgtgaat agagaaaagc tgtctttatt
    11401 ctatttacga ttcacgattc acgatttacg tctttttcct tgtcgcttgt cacttgtcac
    11461 tcgtcacttg tagaatggct ggcatagctt tgcaattcag tttgggcaag cactctgctc
    11521 tctgcgaggg ggtgtcgggt gaagcgtgtt gcgcgtcgcg atcttaacgt cggaggctgt
    11581 gccattcgct aagacgggtg ggctgggcga tgttgcgggc gcgctgccga aggctctcat
    11641 cggcacgggc gaggttgacg ccagcctctg catgccgctc tacgagcaga cggatcgcgc
    11701 gctgctcaaa gaattgctct ttgacaatct ggaagtcgaa tggacgggcg gaaaccgtcg
    11761 cgtccgcgtc ttcctcagtg aagatgcgat tcgcgctccc gcgtttctca tcgacgcgcc
    11821 cgaatacttc gcgcgtccga acgtctacgg ctaccgcgac gaccatacac gctatgcctt
    11881 cttctgtcgc gccgcgctcg cgctcttgaa gcgactcggc aggccgcccg acattgtgca
    11941 cggtaacgac tggccgtgcg gctttgccct cgccgaactg cgcgcgcgcc gcgcctacga
    12001 cgatttttac cgccagacgc gcacgctctt ttccattcac aatctcgctt accagggcgc
    12061 gttcgattcg cgcgatctcg gttacctcgg cttcggttgg ggcgaagttc acgacgcttt
    12121 tctactggaa ggcgcggcat cggcgctcaa agcgggcttg atgctttcgg acgcgctctc
    12181 gaccgtcagc cgccgctacg ctgaagagat acagacgagc gcgcaaggtt acgggctgga
    12241 ctggctgctg cgcatgcgtc gtgaacggct catcggcgtc acgaacggcg tagactacga
    12301 agcgtggaat ccggcaaccg acccgcacat cgccgcacac tacagcgcgg acgatctctc
    12361 aggcaagcgc gagtgtaagc tcgacctgct gcgtcgtttc agtctgcccg aagagttgga
    12421 tcggccagtt atcgcgatca tctcgcgtct cgtggcgcag aagggttacg acctgatccg
    12481 cgaagccgcc ccggcgattc tggagacggg cgcgttcttc atcgcactcg gcgcgggcgc
    12541 atccgagtat gaagactttt tgcagagctt tcacgaccac gcgccgcacc gcgtcggcat
    12601 ctacaaaggc tacgcgggcg aaccgctcgc gcaccagatc gaggcgggcg cagacttgtt
    12661 tctcatgccg tcgctctacg agccgtgcgg tttgaaccag atgtacagca tgcgctacgg
    12721 caccgtcccg gtcgtgcgtg caacgggcgg cctcgacgac acggtggaaa actttgatcg
    12781 tgcgcggggc accggcaatg gcttcaagtt cggcgaatac tctgcgcggg cgatgctcgg
    12841 cagcatctac gaagcgttat acgcttacgc cgacaaagat agctggcggc ggattcaact
    12901 gaacggtatg cgcgccgata attcatgggc ggctgcggcg cgcaagtacg tggaggttta
    12961 tcgcgccatc gcacgtatgt aagacgcagg gcgcgagtag ttcacaggcg agcgcgaaga
    13021 ttgtttgatg aacgcaattt ttaagtaaga gatcatccgc gagcgaattg attcgatcat
    13081 ccggctcgcg tcatgacaag ttggaagaag gagatataac ggcatgagtg aaagtgaatt
    13141 tgtcaaagat gtcagcgaca gcaactttga ggcggaggtg ctacagtcag accgccccgt
    13201 gctcgtggat ttctgggcgg cgtggtgtgc gccgtgccgt atgatcgcgc cgacggtggc
    13261 gcaggtggcg gagaagtatt cggatacggc gcgcgtcgtc aaacttaatg ttgacgagaa
    13321 cccgaacgtc ccgcagaagt acggcatcaa gggcatcccg acgctgatca tgttcaagaa
    13381 cggcaaggaa gaggaacgcg tggtgggcgc gacgaccaaa gaagccatct cgcgcatgat
    13441 cgacaagcac gtgaacgcca gcagcggcac cgctaacgct taagttttat cgtggcagga
    13501 atcggcgcga agttgctgcg gcgaaagcgg ctcgcgccat ttctgtagtc agccgtcaaa
    13561 cagtctgaaa gattgttcaa gctcgcacga ggcgtttgcg cagacggcgg gcgactcccg
    13621 gacgctcgat gaaacaaacc atcaacagcc gcgcgaagaa cgtcggcagc cgctccgcca
    13681 agagcaacaa gcgtcgcctg ctggaagaga gactggcagc ggccaaacgg ctgcgcgccg
    13741 tggaggacgc acgttttcgc gcgcgacaag cgcaagggaa actgcgtcgt ttcgtcagcg
    13801 cgaatttccg caagcaggaa gtgatcgatg cgctcgcgct ccggcgcggc gaatgcaatc
    13861 gttgcggcgc gtgctgcgag atactcttca agtgcccctt cctcaagaag cacgaagacg
    13921 gcacctctac ctgcggcatc tacgaagacc gtcccaatca atgccgcctc tttcccatcg
    13981 aacgccgcga cctcgacgaa gtgcgcggcc agtgcagttt ctacttcatc gagaaaccga
    14041 tcaagctgga acgagccagc tgagaagcct ttgacaggcg acaagtgacc gttgacgggt
    14101 taaaacttgt tctgtcctgt cacctgtcaa tcgtcacctg tcactaaatc ttatgtcaaa
    14161 agtcatcgtt gttatcggcg cgcagtgggg cgacgagggc aagggcaaga tcgtcgacct
    14221 tctggccgaa cgcttcgaca tcgtcgcgcg ctatcagggc gggcacaacg cggggcactc
    14281 ggtctatgtc ggcgaacgcg cgttcgttct ccacctgatt ccttcgggca tcatccatcc
    14341 gggcaaaacc tgcgtgctcg gcaacgggat ggtcatcgac ccgaaagcct tctttgagga
    14401 ggccgaccgc ctcgccgcgc aagggctgga ggtaacgccc gaacgcgtcc tcgtcagttc
    14461 acgcgcgcat ctcatcctcc cctatcaccg cgcgctcgac cacacgagcg aggagagact
    14521 cggcaatgag cgcgtcggga caactttgcg tggcattggt ccggcctacg aagacaaggc
    14581 cggacggcgc ggcatccgtg tcgccgatgc gctcgtgccc gcagttttgc gctcgcgtat
    14641 cgagcgcaat ctggaagacg ccaaccgcat catcgaagcc tacggcgggc aagccttaga
    14701 cgtcgaagag gtcttcaccg aaacttcgcg tctcaccgaa cggctcgccc ccttcattgc
    14761 cgacacgact catttcatga accgcgctgc ccgcgacggt cgcacgatct taatcgaagg
    14821 cgcacaggcc accctgctcg acgtggatca cggcacatac ccgttcgtca cctcttcaaa
    14881 cacgactgcc gggggcgcca gcgccggaac gggactcgcg ccgcaccgca tcacgggcgt
    14941 gctcggcatc gtccgcacgt ataccacgcg cgtcggcgaa ggtcccttcc ccacagagat
    15001 gctcgaaggc gaagccgaac tcggccagtt gatccgcgag cgcggacgcg aatacggcgc
    15061 atccaccggt cgcccccgcc gctgcggctg gttcgacgcc ttcgccaccc gctacgccgc
    15121 ggagatcaac ggcttcacct ccgtcgcgct caccaagctc gacgtgctcg acacactcga
    15181 agagatcaaa gtctgcaccg gatacctgct cgacgggcgc gcggtcgaat ccttccccgc
    15241 cgtctcgcag gacttacgcc gtatcgaacc cgtctacgag acgctccccg gctggaacgc
    15301 ttcaaccgaa ggcatcaccg agatcgaaaa gctgccgccc aacgcacgcg cctacgtcga
    15361 atttctctcc cgcgcgatag acgtggagat cgggctggtt tccaccgggc cggagcgcga
    15421 tcagacgatc ctctgccgtg gttccgcgct cgacgcatgg ctcaaataac aatcgagggc
    15481 ggcacgcttg cccgtccccc gcatcgtatg tacaatgaga cgcaaacgta gcggttgcac
    15541 aaaaagcgtc caatttaact catccgacga gttaatccgc actgccggag acgcaagcgt
    15601 ttgaaatctt cactttgggc cgatagctca gtcggtagag caagtggctt ttaaccactg
    15661 ggtcgcaggt tcgagtcctg ctcggctcat caatccttgc aacggtttag gggcgcgcac
    15721 gcttggcgtc cctaaactat ttcccccttt cgtctacact ttcaagcccg tttatgaaga
    15781 ttcggaggct tatgcgggga cgtttcagtg atgtcgtagt tgtacgtgta tgcggcgcgc
    15841 tcgctactca tcgtctctta cgtgttcggc tccggcctcg cgaaactctt tgctccagag
    15901 cgtgaggagg ttccgttgtg gtagcggggc gcgcaggcca tgatgtcgct cgccgtgctc
    15961 tcgacagcgt ccttcgtgtt gtcgtggctg tacctgttcg cgcgggctac gtggcgggat
    16021 gaataagcgg tggaaagtat tcttaagcgt tttggttgtc aatgattttt agttcgttga
    16081 actgacaaga agttttttct gtataatcct tcattatgaa caaacgagct aaaaagcgat
    16141 tgaaggaagc aattcaaaaa gagattgcgg aacgaacgga taaacgtaat gaacaaactt
    16201 tctctacgcc taccaggaaa gtttcagaga gtagcgggtt taaggtgaag ccggagaaga
    16261 agcggggtta gtttttggtg actacgtaat tggtgagagt taaatctcag gagtagcaat
    16321 gtagagctaa accgctatcc ttatctttca tcaggcggct tttcatcttt cgatgcttcc
    16381 atccaggatg ttatcgaagt taattcgtgc tctccctcta tcttcctgcc aagacgcttt
    16441 tccatctgca ttttatagat acgtgtagcc agccagccga tggcggcaaa tgcaataaag
    16501 agtgtgatgg taattaacat gtgtgactac ccggaagtgc tgtaagaccg gaagagcctc
    16561 cgcttctcct cattccagaa tcggaagtga aaacgcctcc ggccagggtc gtatgcgatg
    16621 gcgaggcgtc tatttttgct cgcagttaaa ttcaagtgtc gaggcggcgt tggcgatctt
    16681 ccagccgtcg gcggtgcgga cgaggtgaaa agagttcgtg ccgcagtgtg agaatttatc
    16741 gccgacgtgg aaagtgtatc gcccgaagac gagagccatg tcgccgaaaa ttttcacttc
    16801 cggtcgcggc attcgttcga tgaactcggc ggctttggcc tccgagatca ttttggcgaa
    16861 agcgtcggca gtgaagacgc gagttttcga caagccttcg cccgtgcgcg gcttgtcgac
    16921 ggcgacgagt tggccttccg gcatgaacag ggcgcgaaga gcttcggcgt tctttgcgcg
    16981 catcgcttca aacaatttgt gcgcgacggc ggcgacggct tcctgtccgg catcgttctt
    17041 cggagcagtc tgcgccaagg ccggagcgta aacggcgaac gtccagatca gcagcaacag
    17101 cgacgataag agtttcttca tagactccgc ttttctcagt gtttctcttc gagcgcgtgt
    17161 tggaagcggg gcgcgatctc gtcgagcgtt ttgtcgagtt ccgcctgtgc gaaccagcgg
    17221 cctttgagca tgacgcccgc gcgtcgctct gtgttcgcga taccggcgag cgggttggct
    17281 tcgagcagca ccagatcggc gcgcttgccc cgttcgatgg tgccggtctt gtccagcgtg
    17341 ccgaaaaact ccgccgggtt tcgcgtggcg gcggcgagcg tggcgtaagg cgagagtccg
    17401 gcttcgttca acgcgcgcag ttcgcggtgc agcgtgtagc cgtagagcaa gagccattcg
    17461 ggcgtgtccg agcctgcgag aattttgccg cccgcgtcgt gaatcgcttt gacgagcttg
    17521 tttctgatgg agacgtaacg cgcgcgtcgt tcgggcgacg cccactgcgc ctgatacttt
    17581 ttgttgtagc cgacccataa atcaaccgtc ttctgcgggt agaagcgtat gtcgggctgc
    17641 aacctgatcg actcttcggt tctcccgatg ccgaaagtcg atttaaagag atgcagcgtc
    17701 ggcacggaga aagggttcgc ctgcaccgtc gcgcgggcta tttcgggaat tctcttttcg
    17761 tccagaacgt cgaggctctc ccagtttttc ggttgataaa tgtagaggtc ggaaaccgat
    17821 cctttgacgg gcgaattttc gggcaacaac gcttcgaggt acgaatcgag atgctcgatc
    17881 tgttgtctgg ctttgagcgc gcggggcagg ccgaccgtgc ggctgtcggc gtggccgacg
    17941 acgcgtatgt tctgccgcgc ggcctcttcg atgatcgctt cgtaaacttc cggcttgagg
    18001 aaagtggtca tcttcaaata atcgtagccg tccgttttgg ccttgcgcac cgctgcgcgc
    18061 gcctccgctt cgttcgtcac gacgtaagcg tgattcgact tgcgaccgat gaactgcggg
    18121 ctggctgcgt agatggtcgg cgcgacgatc tcgcccttcg cggatctcgc gcgcagcacg
    18181 agttgttccg gcgtgccgtt catcaggcga atcgtggtta cgccctgcgc cagcatgatc
    18241 ttgaattcgt cttccgccag cgcgtccggg aattcgtcgt cggtgaacag gtgaacgtgc
    18301 atgtccgtca gtccggggat gagaaacttt ccccgcccgt cgattcgctg cgcgtctccc
    18361 ggcacacgca ccttcttggc gtcgccgatc tccacgatca cgccgtcgcg cacgatcacg
    18421 gtctggttcg acagcacgcg ctcccggtcc atcgggatga cgttcacgcc gacgaaagcc
    18481 gtcgtgttta cagcgggcgt ggcggcctgc ccgaccgacg ccagcagagt taacaggatt
    18541 gataacacga gtctcttttt catcttccgt ccctcgctga acttgagcgt taatctgtaa
    18601 gacctgtcgc catcaacaat ggcggcgaac tgatctcttc tcgatggcct cctgcggaac
    18661 agcgtgccgg gcgtcacggg cgcgccgcct tccgcgctcg tcgtgccgaa gcccttcata
    18721 aacagaacgc gttcgccgct gatgacgacg accgccgcgc ccggtgtgtt attttctctg
    18781 agttcggctg cgacggtctt ttccagttcc gcaaaatcga cgggcttgtg ctgcgccgcc
    18841 gtcgcaacgg cgaacaggaa tagacagcag agggcggaaa ttgaacgcat ggatttcatc
    18901 aaccggtcac acatcattcg cgccctcgcc gttcgcgttg aagaggaagt ctacggcggc
    18961 gcaaagcgtc gcgttgataa cagcagtctc ctgagcgtca gactaaaggg cgtgcgaggg
    19021 tcgagggcgc ggcttctttc gtcagccacg tcgagtccgt gcgttcttaa cgtgtggcgc
    19081 gcctgcttag tttcaaatga gcaggcagag ccgcaaacgg actcattttt atagatgctc
    19141 aagttcattc tgatggatcg atcaggatgg acgacgacgc cgcgacaggc attccggctc
    19201 gactttctcg cccggccaga cgcacttgcg ggaagcggct gcatctaaat ttcggcaggg
    19261 agggctgaag ctatgaacgt gatacaggtt ctcggattgg cgctcggcgc ggcgtggact
    19321 tccggcctca acttgtacgc caccgtggca gtgctcggac tactggagca ctacaaactc
    19381 gcacacctgc ccggcggtct tcacgtactg gacaactggc tgatcatcgg cgtggctctc
    19441 tctctctacg tcgtcgagtt cttcgccgat aagattcctt acgtggacac ggtctgggat
    19501 gcgttgcaca cgttcatacg cgtcccggcg ggggccgcgc tcgcggcggc ggccacctcg
    19561 aacgtcagcc cgacggtaca ggtcatcgcg ctgctgttgg gcggcgggct tgcactctca
    19621 actcacggaa cgaaagctac tgtcagggcg ggagccaatt tgagtcctga gccgctgtcg
    19681 aactggtcgc tctccctgtt ggaggacgtg ctcgccatcg gcggggtggc gttggcggtc
    19741 gtctccccgg tcgggatact ggtcgtcatc ctgatctttc tcctgctcct cgtctggatt
    19801 ctgccgaagg tggtgcggcg tctgcgtcgc atgttgaaag ccgccggaga cttcttcaga
    19861 ggccggacga tggagactcg ctaaagcgag agactaacgc aagggttcct tacccggacg
    19921 gcgcagaaac caaagagaga ggcggggggg gggggggggg gggggggg
//
LOCUS       DASWNS010000356        10281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_507109, whole genome shotgun sequence.
ACCESSION   DASWNS010000356 DASWNS010000000
VERSION     DASWNS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10281
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507109"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VGB76_12545"
     CDS             <1..1060
                     /locus_tag="VGB76_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099768.1"
                     /translation="AVHALALHSVCLMSFVTVQAFAESNAKEAGRAANAALSAQAQEK
                     AKDKKDAPVVPEAELTAAKAIGALTDVQAVIAAASEFLTKYPKSALRPQLAPLVAERI
                     GLTADPVQQIAQAQSYLKIFNAPGEADHVNPHLLAAYTSANRLDEAFALASTALDKSP
                     DPLVSMINLTRGGLEEARKGNLKYIAQSEQYGLKAIDMIESSEKPAAVPDATWADYKT
                     RWLPQLYQWLAMISLQTGDRADAKAKLATAVKLNPLDPINYVVMSSMANDEYQRLAQA
                     HKAATGAEKAELFKKAQAQMDEVIDAYAHAIALAEGNPQYDGLRAQIRPDLEEYYKYR
                     HNNSTEGLQALIDKYKKR"
     gene            complement(1243..1788)
                     /locus_tag="VGB76_12550"
     CDS             complement(1243..1788)
                     /locus_tag="VGB76_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099769.1"
                     /translation="MPTKKTSGKGAASRGGAGKASSKKGGGGGAAGKGGSKKGGAGKG
                     GASKSASKGGGASKSGSQSGGAAKKSTKKAALSSGGSKKGGASKKGGVSKKGGAKSSR
                     KGGGGGGAKKAKGIVRRVLAGAAAGLVSGAVAAVLPPLEEQAGITPVGSNEGQGGGGG
                     GQSGGQSGGSGEGSGGSGGNT"
     gene            2386..6063
                     /locus_tag="VGB76_12555"
     CDS             2386..6063
                     /locus_tag="VGB76_12555"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0099770.1"
                     /translation="MNKLMSFLLLTLLLACAAPAALAQSDRGTLTGTVTDPTGAVVAN
                     AKVTATNLDTGEVRTVTTSDEGNYTMPELSADPYRVTVEATGFKTATMERVQVAVQVT
                     RSADFTLEIGTVGDVVTVTGENAPVIQTENAVRQTNITERQVRELPLAVSAESGGRTP
                     LSFIFLDSNVVSATGTTGRGTDAGNFRVSGGQGQGTEILIDGGGTRRGQNPTFFSEVA
                     PGPNAFQEFTISTSGYSAEFGNSAGGIVNLTVKSGSNELHGEAYDLIRNEAFNANSFL
                     NNSRGSDAAGNPNFPRPRDHQHNFGFNLGAPITLPHFGEGGPYFHSGRNKAFFFFNYE
                     GYRFSQNETVDLTVPTNRMRQGDFGELLTDPYVLGFFGGPVRIYDPRQPINARTPITN
                     NDLRAYQGGALIDPAGLAIMNAYPNPTSPGVFRNYRAVSSRPTTMNNYVGKVDYILSE
                     TQRLGFSYSFRKLDSIKGGFPRFPEPFIAADRWSQFFKSHYARAQYDWSISPTLLNHF
                     NASFTRSEVANRNTTEGFTTSSLGIPANATQNVAFPRVLIFDAGDTERLTNPDPRAYQ
                     QIGSTFFSDNIGDNAVQLSDFVTYVRGRHNLKFGADVRWQQLNVHQLIDPGGTLTFSH
                     LQTASDGDPRSGWPIASLAAGATQNSFAGIRSIDPGWRYFTPAFFVNDDFKVTPRLTV
                     NLGVRYEIPYPRTEARDRYRTFDPDTPNPAAGGRRGALVGAGGQGGLNAQYEGLAKPD
                     YSNIGPRVGFAYSLNDKTVVRGGYGLYYAPIAYNDINQGTIGYSTNRGEGPLGDQRPR
                     FFLRNMPPAFPVDPGGQFIGSGSDVDYIQKDFKTGRTAQFSVDLQRQLPYNFAVSIGY
                     IGSRGNRLRSNFTRLNAIPFEALRLGQPLLTTDLAAALANPAAVAYASSVGVALPSSP
                     NAVYPGFQGTVAQALRPFPQYRNINNQLESQGRSWYNAMQVKLDRRFSQGIQFGAAYT
                     FSKLITNAAEDLLGASPIGGVQQNPFDRESLRTVSPNHPYHVAVFNYIIELPFGKGRR
                     FLNGGGALDKLVGGWQINGIQRYQSGLPLTVFTDRNVFFLRDVTGYLGNLRPNLTGQP
                     ILLDNDPSGTSFRVINPAAFSQPPDFGAPPTTDVSSPAYAAYYANPARFFGNAPPVLD
                     RARALPFYSENLSLLKKTSLTETITLELGAEAFNVFNRHRYFGPETNFANTGGFGFSG
                     VVDNAEVYAPRVVQIRARVFF"
     gene            6165..7826
                     /locus_tag="VGB76_12560"
     CDS             6165..7826
                     /locus_tag="VGB76_12560"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099771.1"
                     /translation="MMSTSFSLFTRILTTAICVVGLGCHADAAHASNYPQRERGASPR
                     SDQSVQLTDAGIAALERGDQVAARELFQRALKANPRNEAAHTYLGIIADRAGALVEAE
                     QHFAAAARHAPASPAAHNNHGAVLLRLGRTAQAAAQFEASLRLDPKQPSALVNLAQIR
                     FASGTPEALRAARELFERAHAIAPEAEVSRALVVIALRLGERQKAVENFRVYAAALGE
                     AKSEVVKPAARAELGAALFEAGLFEEALAELSAAVALDPANADAVLWLALTQRERKDI
                     AAAGRTLEAAIARGIETPALYAALAEVYEASGHIENAIPAMRLAIERDPQNEAYRFRY
                     GMLLTDTKAPAAAIIRLQESLTLFPRSSKLWFALGVAQAASDKYDEAARSFDRALEFE
                     PRFAPALAYLGMTHAQQGRFGEAVALYERALAINDQLAAAHYLLADAMLQQQGADNAR
                     LERHLTRSVELDPAFLPARLALAKLYLRTERATEAVAQLERIVAADPAQADAQYQLGQ
                     AYRRLKRMTEAQTALAAFKRLSETQRTQSQEGQKEIARRLANVRF"
     gene            7901..8587
                     /locus_tag="VGB76_12565"
     CDS             7901..8587
                     /locus_tag="VGB76_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099772.1"
                     /translation="MSTARAQPPAPADKKDAGAQTAAPAQTPALDAWGDTFDGNQLDA
                     SKWEPFSFQGVSGGRVKVEGGELRMRGMHESRAGVRSKRSFTSDRFIVEGTVRKVSPS
                     LPAPGEQGLPLGSAVLNIMFDGSGRNRIEWLLTSEGTFQAWSVVDGVGERLDNRNLAT
                     KIKNPTLAVVRRGDEFLFIINAPGQPAEAAQVAVTKSIRDMPRSFRVMLYGFSSSENN
                     WDSVRVVTTK"
     gene            8599..9741
                     /locus_tag="VGB76_12570"
     CDS             8599..9741
                     /locus_tag="VGB76_12570"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEY0099773.1"
                     /translation="MKHTLRDNIRGGRKNVRVWLLACALLSAALAVAQETRNQDSANR
                     ATQTHAAQTYTNPVYDADFPDPTVIRATDGLFYAYATQAEAAGKTLNIQVARSRDLVR
                     WELLGDALPTKPVWANQTQKFWAPHVSQHGDTFYLYYSADPNTLTGLCLAVATAKQPQ
                     GPFTDIGKPLKCGKSFVNIDPMAFDDPRTGKRLLYWGSGFEPLRVQELAADRVSFKPG
                     SEAREVVSPIKTEDPANYQRLIEGAWVTYRGGFYYLFFSGDNCCGDKAHYAVMVARSR
                     SALGPFETLAEATGEPQSVILELNTRFLAPGHNSVIRDAAGDDWIIYHAIDTNQRNMK
                     AIEGDRDVRRIMLLDRLVYRKGWPRVEWGSPSTLPKRAPAVSAAAR"
     gene            9764..>10281
                     /locus_tag="VGB76_12575"
     CDS             9764..>10281
                     /locus_tag="VGB76_12575"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099774.1"
                     /translation="MNRLKRHLRITASLVLLLFVVVAVPARAQEEGSVKLEGGWQFLA
                     DRAGTLKFNDATRAGGWRNARVGLSWNAQFADLRDYMGVGWYRTSLDVPELKEGRRAL
                     LRFGAVDYFSEVYVNGRQVGTHEGGYTPFEFDVTQAVRAGRNELVVRVVDPPMDEREG
                     RARFPGMLYNEIP"
BASE COUNT         1999 a         3418 c         2994 g         1870 t
ORIGIN      
        1 cgcggtgcat gcccttgccc tccatagcgt ctgcctgatg tcgttcgtca cggttcaggc
       61 gttcgcagag agcaacgcga aagaagcggg acgcgccgcg aacgctgcgt tgtcggcgca
      121 ggcgcaggag aaagcgaaag ataagaagga cgcgccggtt gttcccgaag cggaactgac
      181 agcggcgaag gcgattggag cgttaacaga tgttcaggcg gtgatcgcgg cggcgagcga
      241 gtttctgaca aagtatccga agagcgcttt gcgaccgcaa ctcgctccgc tcgtcgccga
      301 gaggatcggg ctgaccgcag accccgtgca gcagatcgcg caggcgcaaa gttatctcaa
      361 aatcttcaac gcgccgggcg aggccgatca cgtcaaccct cacctgctcg ccgcttacac
      421 ttcagccaac cgtctcgacg aagccttcgc gctcgcctcg accgcgctcg acaaaagccc
      481 agacccgctg gtgtcgatga tcaatctgac gcgcggcggc ctcgaagaag cgagaaaagg
      541 gaacctgaaa tacatcgcgc agagcgagca atatggcctc aaggccatag acatgatcga
      601 aagcagcgag aagcccgcag ccgtcccgga cgcaacctgg gctgattaca aaacgagatg
      661 gttgccgcag ttgtaccagt ggctcgccat gatctctctc cagaccggag acagagcgga
      721 cgcaaaagcc aaactcgcca cggcagtcaa actgaacccg ctcgatccga tcaactacgt
      781 ggtgatgagc agcatggcga acgacgagta ccagcgtctg gcgcaggcgc acaaagctgc
      841 gacgggcgcg gagaaggcag agctgttcaa aaaagctcag gcgcagatgg acgaagtgat
      901 agacgcttac gcgcacgcca tcgcgctcgc ggagggcaat ccgcaatacg acgggctgcg
      961 cgcccagatc agacccgacc tcgaagagta ttacaaatat cgccacaaca actcgaccga
     1021 agggctgcaa gcgttaattg acaagtataa aaaacgctag gccgcgaacg cgccggaagt
     1081 gcggcgacga cgccgcgttg cgctcgtgcg tgcaaagcgc atcggagaag tttgagtccg
     1141 aacaaaagcg gcttcgtgag agattcacga agccgctttt gttttaaacg tcttatcacc
     1201 ggagcgtaac gtctccgcac ggatgttgcg agaaacgttt gactaggtgt taccgccgct
     1261 gccgccgcta ccttcgccgc tgccgccgct ctggccgccg ctttgtccgc cgccgccgcc
     1321 gccctgccct tcattgctgc cgacgggcgt gatcccggcc tgctcttcga gcggcgggag
     1381 cactgcggcc actgcgccgc tcaccagccc ggctgccgca ccggccaaca cacggcgcac
     1441 gatgcctttc gccttcttcg caccgccacc gccgccgccc tttctggaac tcttcgcgcc
     1501 gcccttcttg ctcacgccgc ctttcttgct cgcgccgcct tttttgcttc ctccactcga
     1561 aagggctgcc ttcttggttg acttcttcgc cgcgccgccg ctctggctgc ctgacttgga
     1621 cgcgccgccg cctttggacg cagacttgct cgcgccgccc ttgcccgcgc cgcccttctt
     1681 gctgccgcct ttaccggctg cgccgccgcc gccgcccttc ttagaggacg ctttgcctgc
     1741 gccacctctc gaagccgccc ctttaccact cgtttttttt gttggcatcg ctcgaattct
     1801 ccttgttggt cgtttgagtt tcccggtgtc aacatcctaa tagactaatc cgccgggggg
     1861 cgcaagaaaa aagttggccg cgccgcgtct gcaccctgaa aattctcgac gcttcaacgc
     1921 gcgtcggcaa cccaccggaa cttaaaattc ttccccgcac ctaaattgcg actcgctgcg
     1981 gaagcgaaaa aagagacgcg ccgggaatct tcagccgcgc tgattaaaaa acttgacagt
     2041 ttcgcgcggc ggctgatata agcctcctaa cttggttaac cgtttgcgta atcgcttaag
     2101 cagcctgcgc cgtgtgttct atccagctag actgtaggct tcgaccaagt tcaaactggc
     2161 tctcaataac aatgcgaatc tcaactagca agcgggtgcg tgtgcggagg aatatcgctg
     2221 cgctgcgtcg tctcttctga cgcggttgcg gagtcttgtc gccgctttct catcttttgc
     2281 ggctcccgtc cggtagttga aagttgttct tctgagagca tccgcatttt ctctgtgcat
     2341 atcgagcact cacgttgtct gaggttttta ggaggagtca tgaagttgaa taagctgatg
     2401 tcgtttctgc tgttgacgct cttgctggcc tgcgccgctc ctgctgctct cgcgcagagc
     2461 gaccgtggca cacttactgg tacggtgaca gacccgacgg gtgcggtcgt agccaacgct
     2521 aaagttacag cgacgaacct cgacaccggc gaagtgcgca cggtgactac cagcgatgag
     2581 ggcaactaca cgatgccgga gttatccgcc gatccttaca gggtcacggt cgaggcgacg
     2641 ggcttcaaga ccgcgaccat ggaaagggtg caggtcgccg tgcaggtgac gcgcagcgcg
     2701 gacttcactc tggagatcgg cacggtcggc gatgtcgtga cggtgacggg cgagaacgct
     2761 cccgtgattc aaaccgagaa cgcggtgcga cagacgaata tcaccgagcg tcaggttcgg
     2821 gagttgccgc tggccgtcag cgcggagtcg ggcggtcgca cgccgctttc gtttatcttc
     2881 ctcgacagca acgtggtatc ggccacgggc acgaccgggc gcggaacgga cgcgggcaac
     2941 ttccgcgtct ccggcggtca gggacagggc acggagattc tgattgacgg cggcggcaca
     3001 cgtcgcggcc agaacccgac cttcttctcc gaggtcgccc ccggcccgaa tgcgtttcag
     3061 gagtttacga tcagcacgag cggttattct gcggagttcg gcaactcggc gggcggcatc
     3121 gttaacctca ccgtcaagtc gggcagtaat gaattgcacg gggaggctta cgatctgatt
     3181 cgcaatgaag ccttcaacgc caacagcttt ttgaacaaca gtcgcggcag cgacgcggcg
     3241 ggcaatccca atttcccgcg cccgcgcgac caccagcata acttcggctt caacctcggc
     3301 gcgcccatca ccctgccgca cttcggcgaa ggcggaccct acttccacag cggcagaaac
     3361 aaggctttct tcttcttcaa ctacgaaggc tatcgcttct cgcagaacga gacggttgac
     3421 ctgaccgtcc cgaccaaccg gatgcgtcag ggcgatttcg gcgagttgct cacagacccc
     3481 tacgtgctgg gtttcttcgg tggtcccgtc agaatttacg acccgcgcca gccgatcaac
     3541 gcccgcacgc cgatcaccaa taacgacctg agagcctatc agggcggcgc gctcatcgat
     3601 ccggctggcc tcgccatcat gaatgcttac ccgaatccga cgagtcccgg cgtcttccgc
     3661 aattaccgcg cggtttcaag tcgcccgacg acgatgaaca actacgtcgg gaaggttgat
     3721 tacattctct cggagacgca gcgtctcggt ttcagctact cgttccgcaa actcgacagc
     3781 atcaagggcg gcttcccgcg cttccccgaa ccgttcatcg ccgccgaccg ctggagccag
     3841 ttcttcaagt cgcactacgc acgcgcacag tacgactgga gcatcagccc gacgctgctc
     3901 aaccacttca atgccagctt cacccgctcc gaggttgcca accgcaacac cacggaaggc
     3961 ttcaccacca gctcgctcgg catccctgcc aacgccacgc aaaacgtcgc cttcccgcgc
     4021 gtcctgatct tcgacgcggg cgatacggag cgtttgacca accccgaccc gcgcgcctac
     4081 cagcagatcg gttcgacctt cttcagcgat aacatcggcg acaacgccgt ccaactctcg
     4141 gacttcgtga cctacgtgcg cggacgccat aacttgaagt tcggcgcgga cgtgcgctgg
     4201 cagcaactca acgtccacca gttgatcgat ccgggcggca cgctcacctt ctcgcatttg
     4261 cagacggcct ccgatggcga cccgcgcagc ggttggccga ttgccagtct ggccgcaggc
     4321 gcgacgcaga actccttcgc cgggatcagg tcgattgatc cgggctggcg ttacttcacc
     4381 ccggccttct tcgtcaacga cgatttcaaa gtcacaccgc gcctgacagt gaatctggga
     4441 gtgcgttacg aaattcccta cccgcgcacg gaagcacgcg accgctaccg cactttcgat
     4501 cccgatacgc cgaacccggc agccggtggg cgtcgcgggg cgttggtcgg cgcgggcggg
     4561 cagggcggcc tcaacgcgca atacgaggga ctcgccaagc ccgactattc caacatcggg
     4621 cctcgcgtcg gcttcgctta ctcgctcaac gacaagacgg tcgtgcgcgg cggctacggc
     4681 ctctattacg cgcccatcgc ctacaacgac atcaatcagg gcaccatcgg ctacagcacg
     4741 aaccgtggcg aagggccgct cggcgaccaa cgcccgcgct tcttcctgcg caacatgccg
     4801 cctgccttcc cggtcgatcc gggcggccag ttcatcggca gcggcagcga cgttgactat
     4861 atccagaagg acttcaagac cgggcgcacg gcgcagttca gcgtggactt gcagcgtcag
     4921 ttgccgtaca acttcgccgt ctccatcggc tacatcggca gccgtggcaa ccgcctcagg
     4981 tcaaacttca cgcgcctgaa tgccatcccg ttcgaggctc tgagactcgg acagccgttg
     5041 ctgacgactg atttggcggc ggccttagcc aacccggctg cggtggccta cgcgagcagc
     5101 gtcggagtgg ctttgccgag cagccctaat gcggtctatc cgggattcca gggcaccgtc
     5161 gcgcaagccc tgcgaccgtt cccgcagtac cggaacatca ataaccaact cgaaagtcag
     5221 ggacgcagtt ggtacaacgc catgcaggtc aaactggacc gccgcttctc gcagggcatc
     5281 cagttcggcg cggcgtacac cttctccaag ctgatcacga acgccgccga agacctcctc
     5341 ggcgcgtccc cgattggcgg cgtgcagcag aaccccttcg accgggaatc gctgcgcacc
     5401 gtctcgccga atcaccccta ccacgtagcc gtcttcaact acatcatcga gttgccgttc
     5461 ggcaaaggcc gacgtttcct caatggtggc ggcgcgctcg ataagttggt cggcggctgg
     5521 cagatcaacg gcatccagcg ttaccagtcg gggttgcccc tgaccgtctt caccgaccgc
     5581 aacgtcttct tcctgcgcga cgtgacgggc tacctcggca acctgcgccc gaacctgacg
     5641 gggcaaccga tcctgctcga caacgatccg agcggcacat ctttcagggt catcaacccg
     5701 gctgccttct cgcagccgcc tgacttcggt gcgcctccga ccacggatgt ctcgtcgcct
     5761 gcctacgcgg cctactatgc gaaccccgcg aggttcttcg gcaacgctcc gccagtgctc
     5821 gaccgcgcgc gcgccttgcc cttctacagc gagaacctga gcctgttgaa gaagacctcc
     5881 ctgacggaaa ccatcaccct cgaacttggc gcagaagcct tcaacgtctt caatcgccat
     5941 cgttattttg gaccagaaac gaactttgcg aataccggcg ggttcggctt ctccggtgtg
     6001 gtggacaacg cggaagtcta cgcgccgcgt gttgttcaaa tccgcgcgcg ggtcttcttt
     6061 tagtcctgtt gttctggagt cgtgcggcgc gggaaaagtt gtcaggactt tcccgcgccg
     6121 ttcgcttttt ttgaagccgc cgcactatgt tcgccctgcg gagcatgatg tccacatcgt
     6181 tcagcctatt cacgcgcatc ttgacgacgg ccatctgcgt cgtcgggttg ggctgccacg
     6241 ccgacgctgc ccacgcgtcg aactacccgc aacgagaacg cggcgcgtcg ccccgcagcg
     6301 atcaatcagt tcaattaacc gacgccggga tcgcagccct tgagcgcggc gatcaagttg
     6361 ccgcgcgaga gcttttccag cgcgccctca aagccaatcc gcgaaacgag gcggcgcata
     6421 cctatctggg catcatcgcc gaccgcgcgg gcgcgctcgt cgaagccgag caacacttcg
     6481 ccgccgccgc ccgccacgcc cctgcatcgc ctgccgcgca caacaatcac ggcgcggttc
     6541 tgctccggct cggacgcacc gcacaggccg ccgcacaatt cgaggcttcg ctccgcctgg
     6601 acccgaagca gccgagcgcg ctcgtcaatc tggcgcagat acgctttgcg agcggcacgc
     6661 ctgaagccct gcgcgccgca cgcgagttgt tcgagcgcgc ccatgccatc gccccggagg
     6721 ccgaagtctc ccgcgctctg gttgtcatcg cgctacgtct cggcgagcgg cagaaggcgg
     6781 tcgaaaactt ccgcgtgtac gcagccgcgc tcggcgaggc aaagagcgaa gtcgtcaaac
     6841 ccgccgcccg tgccgaactc ggcgcggcgc tctttgaagc cggactcttt gaagaagccc
     6901 tcgctgaact gagcgcagcc gtcgcgctcg accctgcaaa cgccgatgcg gttctctggc
     6961 tggccttgac ccagcgcgag cgcaaagata tcgccgctgc cgggcgcacg ctcgaagcgg
     7021 ccatcgcgcg cggcatcgaa acgcctgcgc tctacgccgc gctcgcagag gtctatgaag
     7081 cgagcgggca tatcgagaac gcgatccccg cgatgcgtct cgccatcgaa cgcgacccgc
     7141 agaacgaagc ctatcgcttt cgttacggca tgctcctcac cgatacgaag gctcccgccg
     7201 ccgccatcat ccgtctacag gaatcgctta cgctctttcc ccgttcatcg aagctatggt
     7261 ttgcgctcgg cgtcgcacag gcggcgagcg ataaatatga tgaggccgcg cgctcgttcg
     7321 accgtgcgct tgagtttgaa ccgcgctttg ctcccgcgct cgcctacctc ggcatgacgc
     7381 atgcccagca aggccgtttc ggtgaagccg tcgcactcta cgaacgcgcc ctcgccatca
     7441 acgatcaact ggccgccgca cactacctgc tggccgacgc gatgctccag caacagggcg
     7501 cggataacgc tcgacttgag cgacatctga cccgttccgt cgaactcgac cccgcctttc
     7561 ttccggctcg actcgcgctg gcaaaacttt acctgcgcac ggagcgcgcc acagaggccg
     7621 tcgcccaact tgaacgcatc gtcgccgccg acccggcgca ggccgacgct cagtatcaac
     7681 tcggccaggc ttatcgaagg ctgaagcgga tgacggaagc gcaaaccgcg ctcgccgcct
     7741 tcaaacggct cagcgagaca cagcgcacgc agtcgcaaga ggggcagaag gagatcgcgc
     7801 ggcgtctggc taacgtgcga ttctaaaatc acggggagaa ccgttcgtgt ccaaatctat
     7861 caatctgtat ttagccgccc tgctcgtttg ctgctttgga gtgtcaacgg cgcgcgcgca
     7921 accgcccgca ccggcggaca agaaagatgc gggcgcacaa accgccgccc cggcgcaaac
     7981 tcccgccctc gatgcatggg gcgacacgtt cgatggcaac caactggatg cgagtaaatg
     8041 ggagccgttc agttttcagg gagtgagcgg cggcagagtt aaagtcgaag gcggcgaact
     8101 gcgtatgcgc gggatgcacg aatcgcgcgc cggagtccgt agtaagagaa gttttacgag
     8161 cgaccgcttc attgtcgagg gcacggtcag gaaggtcagc ccgtctctgc ccgcgccggg
     8221 ggagcagggt ttgccgctcg gctcagccgt cctcaatatt atgttcgacg ggtctggacg
     8281 caaccgcatc gagtggctgc tcaccagcga gggcacattc caggcatggt cggtggtgga
     8341 cggcgtcggc gagcgtctcg acaaccgcaa tctggcaacc aagatcaaga acccgacgct
     8401 tgccgtcgtg cggcgcggcg atgaatttct cttcatcatc aacgcgccgg gacaacccgc
     8461 cgaagccgcg caggttgcgg tcacgaagtc catcagggac atgccgcgct ctttccgcgt
     8521 catgctctac ggcttcagca gttcggaaaa caactgggac tccgtgcgcg tggtgacgac
     8581 gaagtaaaca ggtcgaagat gaagcacact ttgagagaca acatcagagg cggacggaaa
     8641 aatgtccggg tgtggcttct cgcctgcgca ctgctctccg ccgcgctcgc cgtcgcgcag
     8701 gagacgcgca atcaagattc cgcaaaccgt gcgacgcaaa ctcacgcggc gcaaacttat
     8761 acgaatcccg tctacgacgc agacttcccc gacccgaccg tcatccgcgc caccgacggc
     8821 ctcttctacg cttacgccac gcaggcagag gcggcgggca agacgctcaa cattcaggtc
     8881 gcacgctcgc gcgatctcgt ccgctgggag ttgctcggcg atgcgttgcc cacgaaaccc
     8941 gtctgggcaa accagacgca gaagttctgg gcaccgcacg tcagccagca cggcgacacc
     9001 ttttacctct actactctgc cgacccgaac acgctcacgg gcttgtgcct cgccgtcgct
     9061 accgccaaac aaccgcaggg acccttcacg gacatcggta agcctttgaa gtgcggcaag
     9121 tcgttcgtca acatcgaccc gatggccttc gacgatccgc gcacgggcaa gcgtctgctc
     9181 tactggggtt cgggcttcga gcctctgcgt gtgcaggagt tggccgccga ccgcgtgagt
     9241 ttcaaaccgg gaagcgaagc gcgcgaagtc gtctcgccca tcaagaccga agacccggcc
     9301 aactatcaac ggctcatcga aggcgcgtgg gtcacctatc gcggaggctt ctactatctc
     9361 ttcttctccg gcgacaactg ctgcggcgac aaagcgcact acgccgtgat ggtcgcgcgc
     9421 tcgcgctccg ccctcggccc ttttgagacg ctggccgaag cgaccggaga accgcaaagc
     9481 gtcatcctcg aactcaacac gcgctttctc gcgcccggcc acaattccgt catccgcgat
     9541 gcggcgggcg acgattggat catctatcac gcgattgaca cgaaccagcg caacatgaag
     9601 gccatcgagg gcgaccgcga cgtgcgccgc atcatgctgc tcgaccgcct cgtctatcga
     9661 aagggctggc cgcgcgtcga atggggcagc ccttcgacgc tgccgaagcg tgcgcccgcc
     9721 gtctcagcgg cggcgcgttg aagcggcggg ataaatggga aacgtgaacc gactaaaacg
     9781 tcacctccgg atcacggcaa gcctcgtgct gctgctcttc gtcgtcgtcg ccgtcccggc
     9841 gcgggcgcag gaggagggga gcgtcaaact ggaaggcggc tggcagtttc tcgcagaccg
     9901 cgcgggcacg ctgaaattta acgacgcgac gcgcgcaggc ggttggcgca acgcgcgcgt
     9961 agggctgtca tggaacgcgc agttcgccga tttgcgcgac tacatgggcg tcggctggta
    10021 tcgcacgagt cttgatgtgc cggagttgaa ggaggggcgg cgcgcgcttc tcaggttcgg
    10081 cgcggtagat tatttcagcg aggtttatgt caacggcagg caggtcggca cgcacgaagg
    10141 cggctatacg cctttcgagt tcgacgtgac gcaggccgtg cgtgcggggc gaaacgaact
    10201 cgtcgtgcgc gtcgtcgatc cgccgatgga tgagcgcgag gggcgcgcgc gttttcccgg
    10261 catgctctac aacgaaatcc c
//
LOCUS       DASWNS010000357        12636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_40017, whole genome shotgun sequence.
ACCESSION   DASWNS010000357 DASWNS010000000
VERSION     DASWNS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12636
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40017"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VGB76_12580"
     CDS             complement(<1..292)
                     /locus_tag="VGB76_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY0099775.1"
                     /translation="MPRRRVVDRRDVMPDPVYNSPLVTKFINGLMEGGKKSTAEKIFY
                     NSLLQLGDKVGEDPLKAFKRAIDNVRPQVEVKSRRVGGSTYQVPLEVPQERRG"
     gene            complement(366..788)
                     /gene="rpsL"
                     /locus_tag="VGB76_12585"
     CDS             complement(366..788)
                     /gene="rpsL"
                     /locus_tag="VGB76_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY0099776.1"
                     /translation="MPTINQLVRKGRQEVKYKTASPALQSSPQRRGVCTRVYTQTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGVGHNLQEHSIVLIRGGRVKDLPGVRYHVIRGTL
                     DSVGVADRKQGRSKYGAKRPKGGAAAGAGAKGGAKGKK"
     gene            complement(1399..1656)
                     /locus_tag="VGB76_12590"
     CDS             complement(1399..1656)
                     /locus_tag="VGB76_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099777.1"
                     /translation="MSDEPTKNIEEKYDTRPTIETVLERLDVVAHGLIPLRDEMRDGF
                     VQIDTRLKKVENKISVLNEDVLELRSDVRELKNSLKVHEPR"
     gene            2238..2633
                     /locus_tag="VGB76_12595"
     CDS             2238..2633
                     /locus_tag="VGB76_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0099778.1"
                     /translation="MNLTILYVEDSKFVADAVKETLEDAGWRVETCADGFVALFLIKS
                     ERRYDLILLDNELPNLSGMELTRRTRELPHRKGMPIIMLSASECVRDALLAGADVFLR
                     KPQDIGKIVETVARLLAARTGGSRKMFKR"
     gene            complement(2712..6956)
                     /gene="rpoC"
                     /locus_tag="VGB76_12600"
     CDS             complement(2712..6956)
                     /gene="rpoC"
                     /locus_tag="VGB76_12600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY0099779.1"
                     /translation="MFRFQQEKTQLAPDFEAIRISLASPEKIRQWSHGEVTKPETINY
                     RTFKPERDGLFCARIFGPVTDWECLCGKYKRMKHRGVICDKCGVEVTQAKVRRERLGH
                     IELASPCSHVWFFKGLPSRIGHLLDIPLRELEKVLYFESYIVIDPGDAPVKERELLTD
                     ERYRELMRDFPGAFVAKMGAEAIKELLAMVNIEELSVDLRVKMKDETSQQKKLKYSKR
                     LKVVTSFRKSGNQPNWMILDVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRNNR
                     LKKLMELRAPEVIVRNEKRMLQEAVDALFDNGRRGRVLRGVNNRPLKSLSDTLKGKQG
                     RFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYNQLEKQQYAATI
                     KQAREMVDRQEPVVWDILEEVIREHPVLLNRAPTLHRLGIQAFEPVLVEGKAIKIHPL
                     VCTAFNADFDGDQMAVHIPLSPEAQIEAAVLMLASNNILSPASGQPIAVPSQDIVLGC
                     YYLTRDRAGSKGEGRVFANTDEVLLALDAKEVTTQTPIKLRLAGDLIDLTQEHDTQDI
                     MRATVQENVRRVINTTVGRVIFNDSIPAGLPYINGTLKKKGLTAIVNYCHLRLGHEMT
                     VKMLDDLKTLGFNYATRAGISIGIDDLVTPDAKRVLVGQADTDVIEVDKQYRDGVITN
                     GERYNKVIAIWSEVTDKVAKEMFKAMDKREKDLNELNPILIMSDSGARGTAQQIRQLA
                     GMRGLMAKPSGEIIETPIRSNFREGLNVLQYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIISEPDCGTLRGISSTAIVEGGEEIESLRDRIVGRTALDDVIDPVEGRII
                     VETGAEINEDVAAEIQRSGVQRVRIRSVLTCESRRGVCIKCYGRNLATGRPVDIGEAV
                     GVIAAQSIGEPGTQLTMRTFHIGGTARLEESSKHEARTDGTIHYVELQTVKNRKGEII
                     AMNRNGALTIHDERNREVARFQIVYGAHLHVEEGQRVKQDQLLATWDPFTFAILTEVA
                     GKIKYQDLKEGVTVDEEVDEVTGQSRLVVKDSPDEKKQPRFEIRSLSANKILKTYQMP
                     VRANLMVGDGDEVEPGDVIAKIPRETTKTKDITGGLPRVVELFEARKPRETAIMSEID
                     GIIKLGPIAKGKRKLIVVGNDGEEREYDIPRGTHINVQEGDRVRAGEALMDGPLNPHD
                     ILRVLGMNRLQEYIVNEIQEVYRLQGVNINDKHIEVIVRQMLRWVKIREVGDTEFLLD
                     EQVDRFRFTDENARVAEADGGEQAKAEPLLLGITKASLSTDSFISAASFQETTRVLTE
                     AAISGRIDYLRGLKENVIMGRLIPAGTGMEFYRNVTVDRDETVEHAAHIERGGLDDLP
                     EITGGVDVATPVPATRPTVVAAAAGDGDAAIDEDDE"
     gene            complement(7163..11560)
                     /gene="rpoB"
                     /locus_tag="VGB76_12605"
                     /pseudo
     CDS             complement(7163..11560)
                     /gene="rpoB"
                     /locus_tag="VGB76_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
     gene            complement(11909..12295)
                     /gene="rplL"
                     /locus_tag="VGB76_12610"
     CDS             complement(11909..12295)
                     /gene="rplL"
                     /locus_tag="VGB76_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY0099780.1"
                     /translation="MMATREEVIDYLKGMTLLEASELVKELETVFGVSAAAAAVAAAP
                     AAGGGGDAAAPVEEKTEFDVILQAAGGNKINVIKVVREITALGLKEAKDLVEAAPKAV
                     KEGVTKDEAEAIRQKLADAGATVEVK"
BASE COUNT         2358 a         3727 c         3695 g         2856 t
ORIGIN      
        1 aaccgcgccg ctcctgcggc acttcgagcg gcacctgata ggttgaaccg cccacgcgac
       61 gcgacttgac ttcgacctgc gggcgcacgt tatcaatcgc gcgcttgaaa gccttaagcg
      121 ggtcttcgcc cactttgtcg ccgagttgga gcagcgagtt ataaaaaatc ttctcggcgg
      181 tggacttctt gccgccttcc atcaagccgt tgatgaactt cgtgaccagc gggctgttat
      241 agaccgggtc gggcatcaca tcgcgtctgt cgacaactct tcttcttggc ataattagtc
      301 tcaacgatga acgctgaatg atgaaccctt cttcagttca tcgttccgcg ttcatacttc
      361 aaactttatt tcttaccctt tgcgccgccc ttcgcgcctg cgcctgctgc cgcgccgccc
      421 ttcggacgtt tcgcgccata cttggagcgt ccctgcttgc ggtcggccac gccgacggaa
      481 tcgagcgtgc cgcgaatgac gtgataacgc acgcccggca gatccttcac acgaccgccg
      541 cgaatgagca caatcgagtg ctcctgcaag ttgtggccga cgccggggat gtaggtcgtc
      601 acttcgatcc cgttggtcag gcggacgcgt gccaccttgc gcagagccga gttcggtttc
      661 ttcggcgttt gcgtgtagac gcgcgtgcag acgccgcgcc tttgcggcga cgattgcaga
      721 gcggggctgg ccgtcttgta cttcacttcc tggcggcctt tgcgaacaag ttggttgatt
      781 gtcggcactg ctgatttagt ccttttcgtg cttcccgcta cggggaagtt tcttaaagcg
      841 caaaagactc cgaatcctgc ctctaaccgg aactcgtccg gcagtcagcg caagaagcgg
      901 cggcctctat tgatttaatt aaatctgcgg gatgttctgt ctagcgtttc cgaagaaacg
      961 ggtgtggatc atggctttcc cacgagccaa aatgtttctc tgtggcgtcg cctatcccga
     1021 aggaaaggag tgaggcgcaa actaaaaccg cgccattttt cgcgtgctac gcgctggaat
     1081 ccgcacggtt tctaaccctt aaccgtacgg cgcgcaagca tttacgccac aataattatc
     1141 aagtggctgt ctcaacaaca gacagcccgg caacgcctcc acaccgacag aacctacttg
     1201 agcgctgcac acttgggaaa tggactgtag tccaaaccgg agattgtacg gatcggcaca
     1261 gagtctgtca agttgcggaa agaggctgca ttgtatggct cgaaaatccg cgatataagc
     1321 gtggggacac aagcggaaag agaaagggcg acaccagata gagcgtcgcc cttttactat
     1381 tttccctagg aatatggtct aacgcggttc gtgaaccttc aaactgttct tcaactctct
     1441 cacatcggat cgcagttcca gcacgtcttc attaagcacg ctgattttat tctccacctt
     1501 ttttagacgg gtatctatct gaacaaatcc gtcgcgcatc tcatcacgta agggtattag
     1561 cccatgcgcc accacatcaa gacgttctaa gaccgtttct atcgtcggcc tcgtgtcgta
     1621 cttttcttcg atgtttttgg tgggctcgtc gctcatggag aaagtctagc agaaatgcgc
     1681 aggaatgtgc agggttagag cgtcacggtt acttcttgcc atgtgcggca gacgcgcgct
     1741 tgattccttc gtgcttcgtt gggcttggta gcttctctgg caggtctagc tcgtcgtcaa
     1801 tcagcacatc cacccagacg cctgccgctc gcgcatactc caacaagacc ggaagtggcg
     1861 gttcgcttgt tccgacctca taggcagaca cagtagtccg ggaaagcttg tcactaattc
     1921 ctaaccgttg aagcattccg tcttgtgaga gttcaagcgc gaggcggatt tgtatcaact
     1981 tctccggtag tcttgcgggt ttcggacgag gcacgcgcgc catcagaatg cacctcctga
     2041 tggaacgcta ttcagttcag gcttgtgcct agccagaaat gtggctaaaa ttaactcgcg
     2101 ttcagttgtc gccaagaaga tgcagctatc gtgcttcgtg gcgacgctca tcactcgtga
     2161 gtgggtgagg gtgagctatt caatgcgacc gttgccagta gttttcaaag actcgaacgt
     2221 gagcaagata ccacaagatg aacctcacaa tcctctatgt cgaagatagt aagtttgtcg
     2281 cggatgctgt caaagagacg ttggaagatg caggttggcg ggtggagacg tgcgcggacg
     2341 gctttgtggc tttgttcttg attaagagcg aaagacgcta tgacttgatt ctcttagata
     2401 acgaactgcc taacttgagc gggatggaac tgacacgcag aacgcgcgag ttgccacatc
     2461 gcaaaggcat gccgatcatc atgctctccg caagtgagtg tgtgcgggat gcgctgctcg
     2521 cgggtgcgga tgtgtttctc aggaagcccc aagatatagg caagattgtc gagacggtgg
     2581 cgcgcttgct cgccgcccgc acgggagggt cgcgcaagat gtttaaaagg tgaacgagaa
     2641 aagagcaagg gcgtctccat tcgtggagac gcccttgctc ttggttggtc tatgaaatgg
     2701 aagaggcggt tttactcgtc gtcttcgtca atggcggcgt cgccgtcgcc cgctgctgcc
     2761 gcgacgaccg ttggccgtgt ggcgggcacg ggtgttgcga catctacgcc gccggtgatc
     2821 tccggcagat cgtcgaggcc gccgcgctcg atgtgtgcgg cgtgctcaac cgtctcgtcg
     2881 cggtcaaccg tgacgttgcg gtagaactcc atgcccgttc ccgcagggat gagccgaccc
     2941 atgatgacgt tctctttgag gccgcgcagg tagtctatgc gccctgagat cgccgcttcg
     3001 gtcaggacac gtgtagtctc ctggaacgaa gccgccgaga tgaacgaatc ggtcgagagc
     3061 gacgccttcg tgatgccgag cagcaggggt tcggctttcg cctgttcgcc gccgtcagcc
     3121 tcggccacgc gtgcgttctc gtccgtgaag cggaagcggt caacctgttc gtcgaggaga
     3181 aactcggtgt cgccgacttc gcgaatcttc acccagcgca acatctgacg gacaatcact
     3241 tcgatgtgct tgtcgttgat gttcacgcct tgcaggcgat agacctcttg aatctcgttg
     3301 acgatgtact cctgcaaacg attcataccc aaaacgcgca agatgtcgtg cgggttcagg
     3361 gggccgtcca tcagggcttc accggcgcgg acgcggtcgc cttcctgcac gttgatgtgc
     3421 gtgccgcgcg ggatgtcgta ctcgcgctct tcgccgtcgt tgccgacgac gatgagtttg
     3481 cgtttgccct tggcgatagg accgagtttg atgatgccgt caatctcgga catgatggcc
     3541 gtctcgcggg gtttacgcgc ttcgaacaac tccacgacgc ggggcagacc gcccgtgatg
     3601 tctttcgtct tcgtcgtctc gcgcggaatc ttcgcgatca cgtcgcccgg ttcaacctcg
     3661 tcgccgtcgc cgaccatgag gttggcgcgc acgggcatct ggtaagtctt caggatcttg
     3721 tttgcgctca ggctgcgaat ttcaaagcgc ggctgcttct tctcgtcggg cgaatctttg
     3781 acgacgagac gcgactggcc tgtgacctcg tccacctctt cgtcaaccgt gacgccttcc
     3841 ttcaagtcct gatacttaat cttacccgcc acttccgtaa gaatcgcgaa ggtgaacggg
     3901 tcccatgtcg ccagcagttg atcctgcttg acgcgctgcc cttcttcgac gtgcaggtgc
     3961 gcgccgtaga cgatctggaa gcgcgccact tcacggttgc gctcgtcgtg aatggtgagc
     4021 gcgccgttgc ggttcatggc gatgatctcg cccttgcggt ttttgacggt ctggagttcg
     4081 acgtaatgaa tcgtgccgtc agtgcgcgct tcgtgcttcg aagactcttc gagacgcgcc
     4141 gtgccgccga tgtggaacgt gcgcatcgtg agctgcgtgc ccggctcgcc gatggattgc
     4201 gcggcgatga cgccgaccgc ttcgccgatg tccacggggc gacccgtcgc cagattgcgc
     4261 ccgtaacact tgatgcagac gccgcgccgc gactcgcacg tcagcaccga acggatgcgt
     4321 acgcgctgca cgccggagcg ttgaatctcc gccgccacgt cttcgttgat ctccgcgccc
     4381 gtctccacga tgatgcgtcc ctctacggga tcgatcacgt cgtccagagc cgtgcgcccg
     4441 acgatgcggt cgcgcagaga ctcgatctct tcgccgcctt cgacgatggc cgtcgaagag
     4501 atgccgcgca gcgtgccgca gtcgggttcg ctgatgatca cgtcctgcgc cacgtcaacg
     4561 aggcgacgcg tgagataacc ggagtcggct gtcttcaagg ccgtgtccgc cagacctttg
     4621 cgcgcgccgt gcgtcgagat gaagtattgc aagacgttca agccttcgcg gaagtttgaa
     4681 cgaatcggcg tctcgatgat ctcgcccgaa ggctttgcca tcaaaccgcg catgcctgcg
     4741 agttgtcgaa tctgctgcgc cgtaccgcgc gcgccggaat ccgacatgat caggataggg
     4801 ttgagttcgt tcaagtcctt ctcgcgcttg tccatcgcct tgaacatctc tttggcgacc
     4861 ttgtcggtga cttccgacca gatggcgatg accttgttgt aacgctcgcc gttcgtgatc
     4921 acgccgtcgc gatactgctt gtcaacttcg atcacgtccg tatcggcctg accgacgagc
     4981 acacgcttcg catcgggcgt gacgagatcg tcaatgccga tggagatgcc tgcgcgcgtg
     5041 gcgtagttga agccgagcgt cttcagatcg tcgagcatct tcaccgtcat ctcgtggccg
     5101 agacgcaggt ggcaatagtt gacgatggcg gtcaagccct tcttcttgag cgtgccgttg
     5161 atgtaaggca gacccgcagg gatcgaatcg ttgaagatga cgcgaccgac cgtggtgttg
     5221 atgacgcgac gcacgttctc ctgcacggtc gcgcgcatga tgtcctgcgt gtcgtgctcc
     5281 tgcgtgagat cgatgaggtc gcccgcgagg cgcagtttga tcggcgtctg cgtggtgact
     5341 tctttggcgt cgagcgcgag caacacttcg tccgtgttgg cgaagacgcg tccctcgcct
     5401 tttgaaccgg cgcggtcgcg cgtcaggtag tagcaaccga gcacgatgtc ctgcgagggc
     5461 acggcgatgg gttgccccga cgcagggcta aggatgttat tcgaggcgag catcaagacc
     5521 gccgcttcga tctgcgcttc gggcgagagc gggatgtgga cggccatctg gtcgccgtcg
     5581 aagtccgcgt tgaacgccgt gcagacgagc gggtgaatct tgatcgcttt gccctctacc
     5641 agcaccggct cgaatgcctg tatgccgaga cggtgcaaag tcggagcgcg gttcaaaagc
     5701 acagggtgct cgcggatcac ctcttcgaga atgtcccaca cgaccggctc ctgcctgtcc
     5761 accatctcgc gcgcctgctt gatggtcgcg gcatattgct gcttctcaag ctggttgtag
     5821 atgaacggct tgaacaattc cagagccatc ttcttcggca ggccgcattg atgaagtttc
     5881 aactcgggac cgacgacgat aacggagcga cccgaataat ctacgcgctt gccgagcagg
     5941 ttttgacgga agcgtccctg cttgcccttc aaggtgtccg agagagactt gagcgggcgg
     6001 ttgttcacgc cgcgcagcac gcgaccgcga cgaccgttat cgaagagcgc gtcaaccgct
     6061 tcctgcaaca tgcgcttctc gttgcgcacg atgacttcgg gcgcgcgcag ttccatcaac
     6121 ttcttcagac ggttgttgcg gttgatgacg cgccggtaga gatcgttcaa atcggacgtg
     6181 gcaaagcgtc cgccgtcgag aggcacgagc gggcgaagct cgggcggaat gaccgggatc
     6241 acgtcgagga tcatccagtt cggttgattg cccgacttgc ggaacgaagt gacgaccttc
     6301 agccgcttcg aatatttcag tttcttctgc tgcgaagtct cgtccttcat cttcacgcgc
     6361 aggtcaacgg agagttcttc gatgttgacc atcgcgagca gttccttgat cgcctccgcg
     6421 cccatcttgg cgacgaacgc gccggggaag tcgcgcatca gttcgcggta acgctcgtcc
     6481 gtcaaaagct ctcgctcttt gacgggagca tcacccggat cgatgacgat gtacgactcg
     6541 aaatagagca ccttttcaag ttcgcgcaga ggaatgtcta acaagtggcc gatgcgcgaa
     6601 ggcaagccct tgaagaacca gacgtgcgag caaggtgagg cgagttcgat gtggccgagc
     6661 cgttcgcggc gcactttcgc ctgcgtcact tccacgccgc acttgtcgca gatcaccccc
     6721 cgatgcttca tgcgcttgta cttgccgcac aggcattccc agtcagtcac agggccgaag
     6781 atgcgcgcac aaaacaggcc gtcgcgttcg ggcttgaacg tgcgatagtt gatcgtctcc
     6841 ggcttcgtca cttcgccgtg cgaccattgc ctgatcttct ccggcgacgc gagcgaaatg
     6901 cgaatcgctt cgaagtcggg cgcgagttgc gttttttctt gctggaatcg aaacacgttt
     6961 tatctcctag taagcagtca gccggtaaac aaacaacagt tattaaagcg attggcggtg
     7021 aacgcgtgag ccgtgataca ggctaaaact gctttcggct ccacgcgttc actgcttact
     7081 tttactaacc ttccgtgccg ccgagcagcg cgacgaccgg tttgcccgcg ccgtccgagg
     7141 tttcgtcggg cgtcgcgttg cggtcgatga gttcaacgtc gaggcagagc gattgcagtt
     7201 cgcgcacgag gacgttgaag gattcgggca cgccggggtt gaaatcggct tcgcctttga
     7261 cgatggcctc ataaatcttc gagcgtccgg ccacgtcgtc cgacttggcc gtcaacaact
     7321 cttgaagaat gtgcgccgcg ccgtaggctt ccaaagccca gacctccatt tcgccgaaac
     7381 gctggccgcc aaactgcgcc ttgccgccca gaggctgctg cgtgatcagg ctgtacggtc
     7441 caatcgagcg cgcgtgaatc ttatcgtcca cgaggtgcga gagtttgagc atgtagatgt
     7501 aaccaatggt cactttctgc tcgaacgcct cgcccgatag accgtcgtag agcacggtct
     7561 tgcccgaatc gcctacgatg tccggcaacc cctgctcgcg caaggaggcg gtcgcttcct
     7621 gcaacttctg cttgatctca ggctcggtcg cgccgtcgaa cacgggcgtc gcaaagtgga
     7681 cgccgagcac gcgcgccgcc cagcctaagt gagtctccag aatctgaccc acgttcatgc
     7741 gcgaaggcac gccgagcggg ttcagcacga tgtctacggg cgtgccgtcc ggcaggtacg
     7801 gcatgtcctc ttcgggcagg atgcgcgcga taacgccctt gttaccgtgg cgtccggcca
     7861 tcttgtcgcc gaccgagagt ttgcgtttca tcgcgatgaa gactttgacc atcttgatca
     7921 cgccgggggc gagttcgtcg ccctgtcgca acttcgaaat cttgtcttcg tagatgtccg
     7981 tcagaatttt gacctgacgt tccgtgcgat cttcgtactc tttaacttcc gccgccacgt
     8041 caacgcgcgt cgaagtcaac tgcgctttgc gcagcgcctt cgcgtcgatg cccgcgagca
     8101 tctcgcgcgt gatcgtctcg ttcttcggaa tgcggacttc cttatcgacg gttagatcgg
     8161 cggcgagctt gcgtccttca aagagttcgt agatgcgctg gtcgcgctgt tcgcgcagga
     8221 tgcgcatctc gtcttcgagg tcgcgccgca ggcgattctc ttcctcttcc tcaatcgcca
     8281 tcgaacgctg gtcgcgctcc tgacctttgc gcgtgaagac ctgcacgtca accaccgtgc
     8341 cgtcgatgcc cggaggacac tgcaacgacg catctttcac gtcgccggcc ttctcgccga
     8401 agatcgcgcg caggagtttc tcttccgccg tcagttgcgt ctcgcccttc ggcgtcactt
     8461 tgccgacgag gatcgagccg ggcttgacct gtgcgccgat gcggatgatg ccgctctcgt
     8521 cgaggtcgcg cagcatgttc tcgccgacgt tcgggatgtc gcgcgtgatc tcttcgggtc
     8581 cgagtttcgt atcgcgcgcc tcaatctcca actcttcgat gtggatcgaa gtgtagtagt
     8641 cgtctttgac gagacgctcg gagacgagga tggcgtcctc gaagttgtag ccgcgccacg
     8701 gcatgaaggc gacgagcacg ttgcgcccga gcgcgagttc gcccttgtcc gtgcagggac
     8761 catctgcgat gacctgcccc ttctccacgc gctcgccgat gttgacgatg gggcgttgat
     8821 tgatgcaggt gttctggttc gagcgtttga acttggtgag cgtgtagatg tccgccgtca
     8881 cctcgcgcga gatcgtgccg tcaacgttgt gatcggcttt gacgatgatg cgctcggaat
     8941 ccacgtagtc caccacgccg tcgcgccttg cggcgacgac tgcgcccgaa tcgcgcgccg
     9001 tcacgcgctc catccccgtg ccgatgtacg gggcttcggc gcgcaggagc ggcaccgatt
     9061 ggcgttgcat gttcgacccc atcagcgcgc ggttcgcgtc gtcgttctcc aggaacggga
     9121 tgagcgaagc ggcgacggag acgagttgtt tcggtgaaac gtcaacgtag acgatgtcgt
     9181 cgcgcatggc gagaatgaac tcgcccgctt tgcgcgcggc gttgcgctcg ttgatgatgt
     9241 tgccgtcggc gtcgagttcg atgttggcct gcccgacgac gtatttgtcc tcttcccacg
     9301 ccgtcaggta gaaggggtaa ggctcgtagt cggccttttt gccttctgcg ccgatgcgct
     9361 tgttggcttt ctcgatctct tcctgatgga cgtgatcgcc cggtttgaac ttcgtgtcgc
     9421 cgccgttgat cacacgcacg tattcgacca cgcggccatg ctcgaccttg cggtaaggcg
     9481 actcgatgaa gccgaattcg ttgatgcgcg caaagcacga gagcgacgag atgaggccga
     9541 tgttcggccc ttcaggcgtc tcaatcgggc agatgcgccc gtagtgcgtc gggtgcacgt
     9601 cgcgcacttc gaagcccgcg cgctcgcggg agaggccgcc gggtccgagc gcggagagac
     9661 gacgcttgtg cgtgatctcc gagagcgggt tcgtctgatc catgaactgc gagagttgcg
     9721 acgagccgaa gaattcgcgc acggcagccg tcacgggctt cgcgttgatc agatcgcgcg
     9781 gcatcgtcgt ctgcatctcc tgctggatcg acatcttctc tttgatggcg cgttccatgc
     9841 ggacgaggcc gatgcggaac tggttctcaa ggagttcgcc gacggcgcgg acgcgacgat
     9901 tgccgagatg gtcaatgtcg tctgacatga actcgctcgg attgcgccgc atcttcaaaa
     9961 gataggagat gacctgcacg aaatcgctcg ccgagagaag cgggtcggcg atgcggtcgc
    10021 gctcggggcg tcccatcttg atgttgaact tcaacctgcc gacgcgcgag aggtcgaact
    10081 tgcggtcgtc gaagaacatg ccttcgaaga gccggtaaga agtctgcacg gtgggcgggt
    10141 cgccggggcg catcttgcgg tagatttcga gcagcgcgtc aaccggcttg ttgatcacgt
    10201 ccttcttcaa ggtcaaagag agcaccgagc cgaggtcgtc gcgttcgggg aagaagacgg
    10261 agaagttctg gacgcccgac tccgcaatct cctgcaactt cgcagggttg atctcggtgt
    10321 tggcttcgag caagacctcg cccgtctcca tgttcaccac gtcggcgatg gcatacgcgc
    10381 cctcgaactg ctgcgtgtca acttcgacct ctttgacgtg gcctttgcgc agatcacgga
    10441 gcgcggaggc ggtcactttc tttccggcgc gcgtcacggg ttcgctgtgg ccgcgcacct
    10501 tgagatcgtt gtcaaccttc atgccgatga gattgctcgc gccttcttcg gcgacctgta
    10561 taaagagacg cccgccttcg acccgcactt cgtccaccgt gtagaaggcg cgcaggatgt
    10621 cctcgtcgga gtagcccgcg ttcttcacgg cctcttcgag ttggccgtcc gtgtgaaacg
    10681 ctttcgggtc aaactgcggg ttgagccaga tgcccagagc gcgcaggaag atcgaagcga
    10741 gaaacttgcg cttgcggtcg atgcgtacgt agagcaggtt cttctggtcg tattcgaact
    10801 cgacccacga gccgcgatac ggaatcacct tcgccagata gacgggcgac ttctcgaaga
    10861 agacgccggg cgagcgatgg agttgcgaaa cgatgacgcg ctccgtgccg ttgatgatga
    10921 aagtgccgtt atcggtcatc aacggaattt cgccgaagaa gacttcctct tctttgatgt
    10981 cgcggatgga cttcgcaccc gtctccgggt caacgtcgaa gacggtcagg cgaatcttca
    11041 ccttgagggg cacgctgtag gtcatgccgc gctcctgaca ctcctgctgg tcgtgcttgt
    11101 gcttgaggcc gacaggctcg ccgcagttgt cgcagagttg cgggcgcacg gcattgaacg
    11161 tgccgcagtt gtggcagagc gactcgccgg gcacgagcgg gtcaactttg acgagcgtgc
    11221 cgcaattctt acagttggcg cgcaggtaac gcagaccttc gaggcgaccg catttgcact
    11281 gccagttgcc gatgtggtat tcgacgaaat cgagttccgc cgtgccgcga aaatccgaga
    11341 tcgggaagac cgaacgaaag acggcttgca agccggtcgg ctcgcgctct tccgaaagca
    11401 agtccatctg gagaaaacgg ttgtaggaat cgcgctgaat ctcgatcaaa ttcgggatgc
    11461 gcgccgctgt cttgatcttc gagaagtcgt gccgtgtggg cggttggctc aggcggcgac
    11521 catggccgct tgcgctcgct tgaagcggat ttgcagacat ctttattatt cctttagtga
    11581 caaataacgg gcaggcggcg acaggtcaga acagcttccg ggttttctgt cttatcacgc
    11641 acgcccgtca ttttttgccc ttttcgatag agacgacaaa acgcagccag agcacacaag
    11701 tctctgaccg cttcgataaa agtttagcgg ttgatttttc tcaaactgac tgtgagagta
    11761 tcgccaacgc ctcaatactc ccttacctta gccgctcggc ggctcaactg tcaatcagac
    11821 agtgacaagt gacaggtgac gagtgacaag ttaaaacgaa aagcttctct ttcgctcgtc
    11881 acttgtcact cgtcactcgt cactgttttt acttcacttc gacggttgcg cccgcgtccg
    11941 ccaatttctg gcgaatcgct tcggcctcgt ccttcgtcac gccctcttta acggccttcg
    12001 gagcggcttc gaccaaatct ttggcctctt tcagcccgag cgccgtgatc tcgcggacga
    12061 ccttgatgac gttgatcttg ttgccgccgg cggcttgcag gatcacgtca aactcggtct
    12121 tctcttcgac gggagccgcc gcgtcgccac cgccgcccgc cgccggagcc gccgccaccg
    12181 ccgcagcagc agccgagacg ccaaacacag tctccaactc tttgacgagt tccgaggctt
    12241 ccagaagggt catgcccttc aaataatcga tcacttcttc tctagttgcc atgataacta
    12301 attaacctcc tcaagggttc tgcttgttgt gttcctcgtt tatggttcgc agcaggagaa
    12361 cccacgggac actctcttcc gttcgttgca tccgaacttc cgcaagcgtc cgagccacag
    12421 aggacgacct gacaaatcgc cccgcccgtt ttaaccgcgt ccagctgacc ctacgcgaga
    12481 tttgaaatca caaatctcaa aatcgaattt ctcactaggc agcttccccg ccgccctttt
    12541 gctcgccgat ctgtttaacg acaatcgcca gattgcgcgg cacggctgag agcaccgtga
    12601 ccagacgctg cgcctggcaa ttgatcagga acatga
//
LOCUS       DASWNS010000358         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_323599, whole genome shotgun sequence.
ACCESSION   DASWNS010000358 DASWNS010000000
VERSION     DASWNS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323599"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /gene="hemB"
                     /locus_tag="VGB76_12615"
     CDS             <1..991
                     /gene="hemB"
                     /locus_tag="VGB76_12615"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY0099781.1"
                     /translation="GEREIWMAELVHRPRRLRRREALRALVREARLAREDLVLPLFAC
                     GGEGVRREISSMPGVFNLSVDEVVREASRAFDVGVPAIILFGLPETKDETATGAYARD
                     GVVQAAIRAVRRAVPEMVVIADTCLCEYTSHGHCGVVREGELLNDETLELLAQTAVSQ
                     AEAGADIVAPSAMMDGQVGAIREALDGAGLDQVAIMSYAVKYASAFYGPFREAAASAP
                     AFGDRRSYQMDAANAREAMREAELDYAEGADFLMVKPATAYLDILRMVRDRFDLPLAA
                     YHVSGEYAMIKAAAARGWIDEQRVMMETLTSIRRAGADLIITYYAQDAARLLR"
     gene            complement(1140..2942)
                     /locus_tag="VGB76_12620"
     CDS             complement(1140..2942)
                     /locus_tag="VGB76_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY0099782.1"
                     /translation="MKQNFFFLVLAFVIGAAIILTVREPAARPKTVPPHFVTTSGTRF
                     VLDRQPFRFAGANVAVMYRDEDRARMPETLAEAERLGLGVVRVWAYGEGTEKDGVLPV
                     GRDRDDWPRTHPFRRAPDDWNEEAFVHLDRVLAEAARRNLRVQLCLVNWWRDTGGVTQ
                     YLRWAGIADAADEAHPFGINIERAMLFYTNEEARRLYRQHVERIVTRRNTVTGVLYRD
                     DPTIFAYELMNEAQGLPGRWQERRAWVEEMSRYVKSLDPNHLLTPGTWGYRSAFERRA
                     WLEEHRLPHVDFADVHNYPRDDHDTFVTTTQAFGEFIENRAAASFSLNKPFVMGEFGM
                     GVEGYEGVSQAEWYRAYFQHSAQNGAAGAIFWILTPDPRRGYGITYTDARDETVRAEI
                     ARGSALFANAQHAEPPPVTLDASRHLIPRQFAFTRSASDEGARPAITNPMPGGGLLYR
                     FLPEQAMRGRFEKMGGGSGYVWGTGVGFFEYVVPGREDWRQVGEINVRAHLQPVLPVE
                     AGGRYDGTRVTLYINGTNCGSRLVTVEKPQQTIIQEWRVDSLRPRAEAARGRPLSIRF
                     VVELDADKPFGLNLSNFPTDDHNRDAKPIEVEVR"
     gene            complement(3004..3369)
                     /locus_tag="VGB76_12625"
     CDS             complement(3004..3369)
                     /locus_tag="VGB76_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099783.1"
                     /translation="MLITSTLPVLAAWVQGTPRFGTSLTVIVFLILFVGSLLGWLIAA
                     ILGFARARAFGPSTRWFAFSCLCLLGFNLHLLAVSLFGMTETDPEKVISFGAFSPLFL
                     VLGSISAIIGFLRLTNPRP"
     gene            complement(3648..3863)
                     /locus_tag="VGB76_12630"
     CDS             complement(3648..3863)
                     /locus_tag="VGB76_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099784.1"
                     /translation="MNRILDILYWVVGVIASVGALYYFVKFLRSPGVEGNKNWLWYAI
                     GLAIVACICALLYFVRHVNKEEEIHIT"
BASE COUNT          723 a         1137 c         1322 g          727 t
ORIGIN      
        1 cggggagagg gagatttgga tggctgaatt ggttcatcgt ccgaggaggc tcaggaggcg
       61 ggaggctttg cgtgcgttgg tgcgtgaggc gcgtctggcg cgtgaggacc tggtgctgcc
      121 gctgtttgcg tgtgggggcg agggcgtgcg acgcgaaatc tcttcgatgc cgggggtttt
      181 caatttgtct gtggacgagg tggtgcgcga ggcgtcgagg gcttttgacg tgggcgtgcc
      241 cgcgatcatt ctgttcgggt tgccggagac gaaggatgag acggcgacgg gcgcgtatgc
      301 gcgcgacggg gtggtgcagg cggccattcg tgcggtgcgc cgggctgtgc cggagatggt
      361 cgtaatcgca gacacgtgtt tgtgcgagta cacttcgcac ggccattgcg gcgtggtgcg
      421 cgaaggcgag ttgttgaacg acgagacgct ggaactgctg gcgcagacgg cggtgagcca
      481 ggcggaggcg ggcgcggata ttgtcgcgcc gtcggcgatg atggacgggc aggtcggcgc
      541 gatccgcgag gcgctcgacg gcgcggggct agaccaagtt gcgattatgt cttatgcggt
      601 gaagtacgca tcggcttttt atggtccgtt tcgggaggcg gcggcgagcg cgcccgcgtt
      661 cggcgaccgc cgctcctacc agatggacgc ggccaatgcg cgcgaggcga tgcgcgaggc
      721 cgaactcgac tacgcagagg gcgcggactt cctgatggtc aaacccgcga ccgcttatct
      781 ggacatcctg cggatggtgc gcgaccgttt cgatctgccg ctcgccgcct atcacgtgtc
      841 gggcgagtac gcgatgatca aggccgccgc cgcgcgcgga tggattgacg aacagcgcgt
      901 gatgatggaa accctgacca gcatccgccg cgccggagcc gacctcatca tcacttacta
      961 cgcgcaggac gcggcacgcc tactgagata gagaatgatg aatgaggaac gatgaaggag
     1021 aagagagaat acaggagtca gacgaaagag acgcgcgcgt tttattctga ctcctgaatt
     1081 ctgaattctg actcctgctt ttcattcctc gttcaccgtt ccgccttcaa cgcttttctt
     1141 tatctcactt ccacttcgat tggtttagcg tcgcggttgt ggtcgtctgt ggggaagttg
     1201 gagaggttga ggccgaaagg tttgtcggca tcgagttcga cgacgaaacg gatggagagc
     1261 gggcgaccgc gtgcggcctc ggcgcgcggt cgtagcgagt ccacgcgcca ctcctgaata
     1321 atcgtctgtt gcggtttctc cacggtgacg aggcgcgagc cgcagttcgt cccgttgatg
     1381 tagagcgtga cgcgcgtgcc gtcgtagcgc ccgcccgctt cgacgggcaa cacgggctgc
     1441 aagtgcgcgc gcacgttgat ctctccgacc tgccgccagt cttccctgcc cggcacgacg
     1501 tattcgaaga agcccacgcc cgtgccccac acgtaaccgc tgcctccgcc catcttctcg
     1561 aagcgaccgc gcatggcctg ttccggcagg aagcgataga gcaggccgcc gccgggcatc
     1621 ggattcgtga tcgctggacg cgcgccctcg tcgctcgcgg aacgcgtgaa ggcgaactgg
     1681 cgcgggatca gatgccggga tgcgtcgagc gtcacgggcg gaggttctgc gtgttgcgcg
     1741 ttggcgaaga gcgcggagcc gcgcgcaatc tcggcgcgca cggtttcatc gcgggcgtcg
     1801 gtgtaggtga tgccgtagcc gcgtctgggg tcgggcgtga gaatccagaa gatcgcgccc
     1861 gccgcgccgt tctgcgcgga gtgttggaaa taggctcgat accattcggc ctgcgagacg
     1921 ccttcgtagc cctccacccc catgccgaac tcgcccatca cgaaaggctt gttgagcgag
     1981 aacgaagccg ccgcgcgatt ctctatgaac tcgccgaacg cctgcgtcgt ggtgacgaac
     2041 gtgtcgtgat cgtcgcgcgg gtagttgtgc acatccgcaa aatcgacgtg cgggaggcgg
     2101 tgctcttcga gccacgcgcg ccgctcgaag gccgagcgat agccccaggt gccgggcgtc
     2161 aacaggtgat tcggatcgag cgacttcacg taacggctca tctcctcgac ccacgcgcgc
     2221 cgctcctgcc agcgaccggg aagcccctgc gcttcgttca tcaactcgta agcgaagatc
     2281 gtcgggtcgt cgcggtagag cacgcccgtc acggtgttgc ggcgcgtgac gatgcgttcg
     2341 acgtgctgac gatagaggcg acgcgcctct tcgttagtat aaaagagcat cgcccgttcg
     2401 atgttgatgc cgaaggggtg cgcctcgtcc gccgcgtcgg cgatgcccgc ccagcgcagg
     2461 tattgcgtga cgccgcccgt atcgcgccac cagttgacga ggcagagttg gacgcgcagg
     2521 ttgcggcgcg cggcttcggc gagcacgcgg tcgagatgga cgaaggcttc ttcgttccaa
     2581 tcgtcgggcg cgcggcgaaa agggtgcgtg cgcggccagt cgtcgcggtc gcgcccgacg
     2641 gggagcacgc cgtctttctc ggttccctcg ccgtaagccc agacgcgcac gacgccgagg
     2701 ccgagacgtt ccgcttccgc cagcgtctcc ggcatccgcg cgcggtcttc atcgcgatac
     2761 atgacggcga cgtttgcgcc cgcgaagcga aagggttgtc ggtcgagaac gaagcgcgtg
     2821 ccgctcgtcg tgacgaaatg aggcggcacg gtcttgggtc gcgcggcagg ctcgcgcacc
     2881 gtcaggatga tggctgcgcc gatgacgaag gcgagcacca gaaaaaagaa gttttgtttc
     2941 aatgcgaaaa gtgagctttc taaaattgat ttaccgaaac gagacggaag cgcacgggga
     3001 gtttcaaggg cgtggattgg tcagccggag gaaaccgatg atggccgaga tactgccgag
     3061 cactaagaag aggggcgaga acgcaccgaa gctgatcacc ttctccgggt cggtttccgt
     3121 catgccgaac agggagacgg cgagcaggtg cagattgaag ccgagcaggc agaggcaact
     3181 gaacgcgaac cagcgcgtgg aaggtccgaa cgcacgcgca cgcgcgaaac cgaggatggc
     3241 cgcgatcagc caaccgagca gcgagccgac gaataagatc aaaaagacga tgactgtgag
     3301 cgacgtgccg aagcgcggcg tgccttgcac ccacgcggcg agcacaggga gagttgaagt
     3361 gatcagcatt cgtttttttt tccttcgttg gccgaccgcc ggaagagagc cgacacaaga
     3421 aaagcgccgt tcgcgcggag cctgttgcta cagccacacg ttcgcgaacg gcgtcgaagt
     3481 tttcatgtct gacagcgaca gcgcgtgctg gctctgtccg gcgtttcgcg tgttcctact
     3541 ccgacgcgcg atgcgttagt ttcccgaccg acgccgggaa tcaacgcgcc cgcgcgcact
     3601 cttctcgctc atgcggcgac caagcgttcg ccgcctccac tcacagctta cgtgatgtga
     3661 atttcctctt ccttgttgac gtgccgcacg aagtagagca gcgcacagat gcaggccacg
     3721 attgccagtc cgatggcata ccacagccag ttcttgttgc cttcgacgcc gggcgatctc
     3781 aaaaacttca cgaagtaata gagcgcgccc acgctggcga tgactcccac cacccagtag
     3841 agaatgtcca gaatacgatt catgtttgac cctccggcgt ttgttgttaa tcgaaatgat
     3901 ggctagcga
//
LOCUS       DASWNS010000359        12695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_482284, whole genome shotgun sequence.
ACCESSION   DASWNS010000359 DASWNS010000000
VERSION     DASWNS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482284"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VGB76_12635"
     CDS             <1..985
                     /locus_tag="VGB76_12635"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0099785.1"
                     /translation="VDRGIIGRSEKMQAIFQLLDQVAPARSTVLITGESGTGKEILAR
                     AIHDQSPRAAKAFMPVNSSNIPSDLLESELFGHTRGAFTGAVAAKKGLFEVADGGSVF
                     LDEIGNIPPETQARLLRVIQEREFTPLGDTTARRVDVRIIAATNIDLKEAVRQGTFRE
                     DLYYRLAVVPIELPPLRERREDILPLAQHFIRKYNEENARHISDQLAPDVLALMEDYT
                     WPGNVRELENVMERAVVIAPTNEVTCQCLPTEICEPQKSLGASTSPSAAGVDVGRGID
                     FYDEVRRFEVDLIRRALKQTGGHQSRAARLLGMNATTLNSKIKTYNIQLRA"
     gene            1052..1975
                     /locus_tag="VGB76_12640"
     CDS             1052..1975
                     /locus_tag="VGB76_12640"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEY0099786.1"
                     /translation="MADSRATGARAEVWPALPLEEWKDTYATLHMYTQIVGKIRLVQS
                     PPINHWWQVPLYVSARGLTTTAIPHGTRNFEIEFDFIEHRLVIRTSEGETRNLPLAAR
                     PVGDFYRTLMATLAALGLEVKIRAIPDELPNPIPFAEDYEHASYDAEYVQRLWRILTQ
                     TDRVLKEFRSRFIGKVSPVHFFWGSFDLAVTRFSGRPAPQREGADSITREAYSHEVIS
                     HGFWPGSGNIEMPAFYSYTAPEPAGLPQAAIRPGSAFYNPPTGGFILPYDDVRQADEP
                     EQVLMEFLQSTYEAGANLAGWERAALERTSS"
     gene            complement(2158..2544)
                     /locus_tag="VGB76_12645"
     CDS             complement(2158..2544)
                     /locus_tag="VGB76_12645"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0099787.1"
                     /translation="MTRKLFLALAISLSLFGATPGMAQTGAGIQNRGHAPTRPDGLGR
                     LDLRVTDAQGQAIKGAQAKLTSQRPGGFVCETWNSSDANGVAVLPPLHVGKLKLVVKA
                     AGYRTQTLEIAPNALSQPVRVMLVRA"
     gene            complement(2710..4374)
                     /locus_tag="VGB76_12650"
     CDS             complement(2710..4374)
                     /locus_tag="VGB76_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099788.1"
                     /translation="MPTTSSAVGADERVLLLAPTGRDAQMISKVLGEAGVGTEACSDI
                     NDFCRKMSEGAGAAFITEEALTTTALKCLVEALAEQPPWSDFPLVLLTSGGENSAVNL
                     RVLKTLGEAGNVTIVERPTRIITLVSALHASLRARRRQYEVREHLLAQKRAEEERALL
                     LAEAEAANRTKDEFLATMSHELRTPMTAILGWAQLLRSNTLKESDFSHALEIVERNAR
                     AQTKLIDDLLDISRIITGKLRLDVRVVDLASVIEAVIDSLRPTAEARAIRLQPLLDPQ
                     AGPVSGDTDRLQQVVWNLVSNAIKFTPKGGRVQVMLARINSHVEITVSDTGKGISAEF
                     LPHVFDRFRQADGTTTRRQGGLGLGLSIVRQLVELHGGAVSVESVGEGQGATFIVQLP
                     LMATRRRSDAGDGARWRHPTAGGDDASFDCPPSLEGLRVLVVDDEADTRGLLRKVLEG
                     CGSEVMTAGSVAEALESIAQTKPDVLISDIGMPEEDGYELIGKLRALETGRAGRIPAI
                     ALTAYARVEDRVRALTAGFQVHVPKPIEPAELIAVVASLTGRTGNS"
     gene            complement(4361..5848)
                     /locus_tag="VGB76_12655"
     CDS             complement(4361..5848)
                     /locus_tag="VGB76_12655"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY0099789.1"
                     /translation="MTARLGTGSVGFDSILEGGFPPNHLYLIEGDPGTGKTTLALKFL
                     LEGAAQGEPVLYVTLSETKEEIKAVAASHGWSLDGMSIYELVPSEDSLKTEMQYTIFH
                     PSEVELGETTNAVLEEVGRIQPRRVVFDSLSEMRLLARDPLRYRRQILALKQYFAGRQ
                     CTVMLLDDRTSTASDLQVQSIAHGVVELEHLALDYGAERRRLRIAKLRGSTYRGGFHD
                     FKIETGGVVVYPRLVASEHQQQFAPEMVTSGVAELDALLGGGLNRGTSTLVLGPAGSG
                     KSSIAAQFAAAAAERGEHAASFLFDEGLQTYLSRASGLGREMSEQVKSGRMSLRQVDP
                     AELSPGEFAYYVRQAVDRDRARVVVIDSLNGYMQAMPDERFLTVQMHELLSYLNRQGV
                     VTLLVLAQHGFMGSNMGTPVDVSYLADTVLMLRFFESRGAVRRAISVVKKRTGYHENT
                     IREMQLSSEGIKVGQPLNDFQGVLTGVPTYSKQAASRLEETDADD"
     gene            6060..7580
                     /locus_tag="VGB76_12660"
     CDS             6060..7580
                     /locus_tag="VGB76_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEY0099790.1"
                     /translation="MNRTLDVVEQITEGEARFERVRRVAGLALAPAIFILLLLLPLPA
                     LKPEAHRLAAVMAAVVVLWVTEALPLPVTALLGAAACVVMRVAPARDVFAPFADPLMF
                     LFIGSFIIARAIFLHRLDRRLAFGVLSIKWVGARPGRILVAFGAVTAFISAWISNTAT
                     TAMMFAIGMAILAFLFDNEREGGSPINRRYATGLMLMTAFAASIGGLATPIGTPPNVI
                     GLGFIRQLVGIEFPFFKWAMIGVPVVIILFLYLSFYLNALCRAGVREIEGSREMLLRE
                     RSRLGAWTRGQKSTLVAFLATVALWIVPGLIALFAGDQSAIYQAVNRRVPEAVAAVVG
                     ASLLFLLPGDGRGERAITWDEAVKIDWGVVLLYGGGFALGVLSFQTGLAEAIGYGLTK
                     HLPISGGLSLLFVSTLVATLTSEATSNTASANMVVPVVIAISRAAGADPLEPALGATM
                     GASLGFMLPVSTPCNAIVYGSGYIPLGRMIRYGLLLDVVGVVVIVAVVRLLVPFLR"
     gene            7735..8682
                     /locus_tag="VGB76_12665"
     CDS             7735..8682
                     /locus_tag="VGB76_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY0099791.1"
                     /translation="MERIVFLDRNTLRAKIRPPAFAHEWREYGATRAEEVVERLEAAS
                     IAIVNKVEIRDAHLAQLPALRLIAVAATGTDNIDLKSCRERGVAVSNVRGYAAGTLPE
                     HVFMLMLALRRNLVSYRTDVRAGRWQRAEGFCLLDHPIRDLHESTLGIIGYGALGRAV
                     EQLARAFRMHTLIAEHKGAQHTRAGRTPFAEVLRTSDIVTLHTPLTAETRHLIGAPEL
                     ALMKPSALLINCARGGILDEAALVEALQAGVIAGAGVDVLSREPPREGNPLLELDLPN
                     LIVTPHVAWASLEAMQTLADQLIENIEAFIKQRRVTSGE"
     gene            complement(8803..9075)
                     /locus_tag="VGB76_12670"
     CDS             complement(8803..9075)
                     /locus_tag="VGB76_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099792.1"
                     /translation="MAENENALGAAEATPDGQTGGSEASGAAPAGEAAAPESTEEITY
                     HAVEKDGQVTALWEMQGRKHLRTIDPRSRQGQEILRLYTAEHHDEQ"
     gene            complement(9258..10610)
                     /locus_tag="VGB76_12675"
     CDS             complement(9258..10610)
                     /locus_tag="VGB76_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099793.1"
                     /translation="MRLNPETVLKRCPYLRLSINSSNEVQVSVEGKLIKCGLHALSIL
                     EMFSAPLTVAAAIKQLGARVASADRVAAIATLFRLYEGGALRDVAAAAARERATGAAG
                     DESAQTADDAVTVEASTVAASGDDVGENFASDEIYFACPLDDSSRRLIPYRERVEREG
                     CAETTPLESLTLNSRIALQTGDELPARFAARLPFLDRFSPERPLLWVEDAGTQVLAPY
                     WLSPAQAELVRRLEAGASAPSELETETARLFASAQILLAPGEAEERAKRWQRACEELR
                     SCLQTRQHAVLRKIIHPLQLAALRTYFRALDARGHLKASSNKHYAHNRRSTYDEESSR
                     FVQQQINALVNRITPAPVRPSHCQVSVYQPGAFLRRHKDQPQYVWNLSLVVDAEPELE
                     RASAWPINLEVEGRTEEVRLEIGDAVLYRGSEFTHWRETLPEGQSVTLLFCFFSPAND
                     "
     gene            complement(10611..12380)
                     /locus_tag="VGB76_12680"
     CDS             complement(10611..12380)
                     /locus_tag="VGB76_12680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0099794.1"
                     /translation="MRDCRLQDGSTVVIIGGGPGGAACAIKLLQGAQARSLRLRVLIF
                     EGKDFNVHANQCVGVLSPPIEEVLARDLDVHLPRTLIKRQIFGYRLHGARENVLLMGH
                     GAHASEAGHGQATYAVERAEFDRFLLDQARALGAEVVGSRVTGVEFVREGELDEVRVY
                     SESHYERADAVVGAFGLDEAMLSVFEEVTGRGRGARGFVRPSKWLKSYLTTIEAGHGF
                     QGEKLGHIVHAFLLPPRCPRIEFGAVTPKGDHILINIAGERVSSSDLDQFLQLPEVRA
                     LLPAFDERAINYYEGRFPSAPARGAYGHRYALVGDATGWLRPFKGKGISTAIITGLRA
                     AETMLEHGVSREAGARYARACRDLFGDYAYGVAVRRLMLAGARVFLDPMIDVGKVDPL
                     MYDALFDAVSGHETYRNIIRRSFKPRLVRKVATRVFGGVRRHRAFRARRMKDIKVRRM
                     TVRDIDELLRINEKITGKPHAAYYETKAADYIGRSPTTCLVAEHGGRVVGFVMGDVRG
                     WEFGAKLAGWLEVIGVDPSYHNRGVSSALMDALCESFRRANVSVVNTIVNWNDGDLID
                     YFRANGFDRGDYVNLVRNLDEQS"
     gene            12392..12544
                     /locus_tag="VGB76_12685"
     CDS             12392..12544
                     /locus_tag="VGB76_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099795.1"
                     /translation="MKVFPCEVRLLSEVRLPPLRRARKQGRHLIACDAGAEIYLPVYS
                     PYLPRA"
BASE COUNT         2292 a         4036 c         3913 g         2454 t
ORIGIN      
        1 cgtggaccgc ggcatcatcg gtcgctccga gaagatgcag gccatcttcc aactcctcga
       61 tcaagttgcg cccgcgcgct ccaccgtgct catcacgggc gagtccggca cgggcaaaga
      121 gattctcgca cgcgccatcc acgatcaaag cccgcgcgcc gcgaaggctt tcatgcccgt
      181 caactcttca aacattcctt cagacctttt agagtcagaa ctcttcggcc acacgcgcgg
      241 cgcgtttacc ggcgcggtgg ccgccaagaa gggactcttt gaagtggccg acggcggctc
      301 ggtctttctc gacgagatcg gaaacatccc gccggagact caggcgcggc tgctgcgcgt
      361 gatacaggaa cgcgagttca ccccgctcgg cgacacgacg gccagacgcg tcgatgtgcg
      421 catcatcgcc gccaccaaca tcgatcttaa ggaagccgtc cgtcagggaa ctttccgcga
      481 ggacttatat taccggctcg ccgtcgtgcc tatcgaactg ccccccttgc gcgaacggcg
      541 cgaagacatc ctcccgctcg cgcagcattt catacgcaag tataacgaag agaacgcgcg
      601 ccacatctcc gatcaactcg cgcccgacgt gctcgccttg atggaggatt acacgtggcc
      661 gggcaacgtg cgcgaactgg aaaatgtgat ggagcgcgcc gtcgtcatcg cgccgaccaa
      721 cgaagtgacg tgtcaatgtc tgccgacgga gatttgcgag ccgcagaagt cgctcggcgc
      781 gagcaccagc ccttcggcgg cgggcgtgga tgtcgggcgc ggcatagatt tttacgacga
      841 agtgcgccgc tttgaagttg acctcatccg ccgcgccctc aaacagaccg gcggccacca
      901 atcccgcgcc gcccgtctcc ttggcatgaa cgcgacgacg ctcaactcca agatcaagac
      961 ctacaacata cagttgcgcg cctgaaaccc ctcgcctttg caacgaactc tgacgcgcag
     1021 cgggcgcgag catttgaagg gagattgacg aatggctgac tccagggcga cgggcgcgcg
     1081 tgcagaagtt tggcctgccc tgcccctcga agaatggaaa gacacctacg cgacgctgca
     1141 catgtatacg cagatcgtgg gcaagattcg gctcgtgcaa agcccgccca tcaaccactg
     1201 gtggcaggtg ccgctctacg tctccgcgcg cgggttgacg acgaccgcca tcccgcacgg
     1261 gacgcgaaac ttcgagatcg agttcgactt catcgagcac cgactcgtca tccgcacgag
     1321 cgaaggcgag acgcgcaacc tcccgctcgc cgcgcgcccg gtgggcgact tttaccgcac
     1381 gttgatggcg acacttgccg cgctcggcct cgaagttaaa atccgcgcga tacccgacga
     1441 actgccgaac cccattccct tcgcggaaga ctacgaacac gcttcatacg acgccgaata
     1501 cgtgcagcgt ctctggcgca tcctcacgca gactgacagg gtgctcaagg agtttcgttc
     1561 gcgcttcatc gggaaggtga gtcccgtaca tttcttctgg gggagtttcg atctggccgt
     1621 cacgcgtttt tccggacgcc ctgcgccgca gcgagaggga gcggattcca tcacacgcga
     1681 agcctactcg cacgaagtca tcagtcacgg cttctggccg ggcagcggca acattgagat
     1741 gcccgctttc tactcctaca ccgcgcccga acccgcaggc ttgccgcagg ccgcgattcg
     1801 tcccggcagc gcgttctata accccccgac cggcgggttc atcctgccct acgacgacgt
     1861 gcggcaggcc gacgaaccgg agcaagtgtt gatggagttt ctgcaaagca cctacgaggc
     1921 gggcgcgaac ctcgccgggt gggagcgcgc cgctctggaa cgaacgtctt cgtaacgtgt
     1981 taattctgcc cgtccgaccg tgaggctggc ggcgaggctc cgctccaaaa gcagaactta
     2041 caacaccggg tcgcgctctc aaccgacgcc aactcccgac agcacaaagc ccgcgacgct
     2101 tggaagtcat agcttccgag cgtcgcgggc tttcttcgag ttactttacc gcttggttta
     2161 agcgcgaacg agcatcacgc ggacgggttg ggagagtgcg ttcggcgcga tctctaaagt
     2221 ctgcgtccgg tagcccgccg ccttgacgac gagtttcagt ttgccgacat gcaagggcgg
     2281 gaggacggcc acgccgttcg cgtccgaact gttccacgtt tcgcagacga aaccgccggg
     2341 acgctgcgat gtgagcttgg cttgcgcgcc tttgatggct tgcccctgcg catctgtgac
     2401 gcgcagatca agtcgcccga gtccgtcggg acgcgtcggc gcatgcccgc gattttgaat
     2461 acctgcgccc gtctgagcca tgcccggagt tgcgccgaaa agtgacagag aaatagctaa
     2521 ggcgagaaac agttttcgcg tcatttattc aaaaactcct gattttataa gtgtttagct
     2581 cacggggggc gtcggggaat tagatgtgcg agggcgtgca aagttgcctg tcgaagagca
     2641 tgtttcgacg cgccgcacgc tatctccgac ccgcgccgta aattgcgtct gacgcgtcgt
     2701 agcggatgct caactgttgc ccgtccgccc tgtgaggctg gcgacgacgg caatgagttc
     2761 ggctggctca atcggtttcg ggacgtgcac ctgaaagccc gccgtaagcg cgcggacgcg
     2821 atcttccacg cgcgcgtagg ccgtcagcgc gatggccggg attctccccg cgcgtcccgt
     2881 ctccaacgcg cgcagttttc cgatcagctc gtagccgtcc tcttcgggca tcccgatgtc
     2941 gctgatgagc acgtccggct tggtctgcgc gatggattcc aacgcctccg ccacagaccc
     3001 ggcggtcatc acttccgagc cgcacccttc gagcactttg cgcagcagcc cgcgcgtgtc
     3061 cgcttcatcg tcaacgacga gaacgcgcaa gccttcgagc gaaggagggc aatcgaagga
     3121 ggcgtcgtct cctccggcgg tcgggtgcct ccagcgcgcg ccatcgcccg cgtcgctcct
     3181 ccggcgcgta gccatcagag gcaactgcac gatgaaggtt gcgccctgcc cttcgcccac
     3241 gctctccacg ctgaccgccc cgccgtgcag ttcgacgagt tgacgaacga ttgagagtcc
     3301 taagcccaaa ccgccctgcc tgcgcgtcgt cgtgccgtca gcctgccgga aacggtcgaa
     3361 gacgtgcggc agaaactccg cgctgatgcc cttgcccgta tcgctcaccg tgatctcgac
     3421 gtgcgagttg atgcgggcga gcatgacctg cacgcgcccg cctttcggcg tgaacttgat
     3481 ggcgttcgag acgagattcc agacgacctg ctgcaagcgg tctgtgtcgc cggagactgg
     3541 accggcctgc gggtcgagca gcggttggag acgtatcgca cgcgcctccg ccgtcgggcg
     3601 cagcgaatca atcaccgctt cgatcaccga cgcgagatcg actacgcgca catccagacg
     3661 cagttttccc gtgatgatgc gcgagatgtc gagcaggtcg tcaatgagtt tggtttgcgc
     3721 gcgtgcgttg cgctcgacta tttccagggc gtgcgagaag tcggattctt tgagggtgtt
     3781 gctgcggagc agttgcgccc aaccgaggat ggcagtcatc ggcgtgcgga gttcgtgcga
     3841 catggtggcg agaaactcgt ccttcgtgcg gttggcggcc tcggcttccg ccagcagcag
     3901 ggcgcgttct tcttcggcgc gcttttgtgc cagcaggtgc tcacgcactt catactggcg
     3961 gcggcgcgca cgcagagatg catggagcgc gctcacgagc gtaatgatgc gcgtcgggcg
     4021 ttcgacgatg gtgacgtttc cggcctcgcc gagagttttc aggacgcgca gattgacggc
     4081 ggagttttcg ccgccgctcg tcagcaggac gagcggaaag tctgaccagg gaggctgttc
     4141 ggccaacgct tcgaccagac atttcaaagc cgtcgtcgtc agagcctctt ccgtgataaa
     4201 ggccgcgccc gcgccttcgg acatcttccg gcaaaaatca ttgatgtcgc tgcacgcctc
     4261 tgtcccgacg cccgcctcgc cgagcacttt gctgatcatc tgcgcgtccc ggcctgtggg
     4321 cgcaaggagc agcacgcgct cgtccgcgcc cacggcggaa ctagtcgtcg gcatccgttt
     4381 cctccaaacg cgacgcggcc tgtttgctgt aggtcggcac gcccgtcagc acgccctgaa
     4441 aatcgttgag cggttgcccg accttgatcc cttcggacga gagctgcatc tcgcggatcg
     4501 tgttctcgtg atagcccgtg cgtttcttga cgaccgagat ggcgcggcgc accgcgcccc
     4561 tcgactcgaa gaagcgtaac atcaacaccg tgtcggccag ataactgaca tcgaccggag
     4621 tgcccatgtt gctgcccatg aagccgtgtt gcgccagaac gagaagcgtc accacgccct
     4681 gccggttgag atagctgagc aactcgtgca tttgcaccgt gaggaaacgc tcgtcgggca
     4741 tggcctgcat gtagccgttc aaactgtcga tgacgacgac gcgcgctctg tcccggtcta
     4801 cggcctgacg cacgtaatac gcaaactcgc cgggcgacag ttccgccgga tcgacctgcc
     4861 ggaggctcat gcgtcccgac ttgacctgct cggacatttc tctgccgagt cctgaagcgc
     4921 gcgagaggta agtttgcaag ccttcgtcga agaggaaaga ggcggcgtgc tcgccacgtt
     4981 cggcggcggc ggcggcaaac tgtgccgcaa tcgaagactt gcccgaaccc gccggaccga
     5041 gcacgagcgt actcgtgccc cggttgagtc cgccgcccag aagcgcgtcc agttccgcga
     5101 cgccgctcgt caccatctcg ggcgcaaact gctgctggtg ttccgacgcg acgaggcgcg
     5161 ggtagaccac cactccgccc gtctcaatct tgaaatcgtg aaagccgccg cgataggtgc
     5221 tgccgcgaag tttggcgatg cgcaggcgac gcctctccgc gccgtaatcc aaagccagat
     5281 gctccagttc gacgacgccg tgcgcgattg attgcacctg caagtcgcta gccgtcgagg
     5341 tgcggtcgtc cagaagcatg acggtgcatt gccgccccgc gaagtattgt ttgagcgcga
     5401 gaatctgccg ccggtagcgc agcgggtcgc gcgcgagcag gcgcatctcc gagagcgaat
     5461 cgaagacgac gcggcgcggc tggatgcgtc ccacctcttc gagcaccgcg ttcgtcgtct
     5521 cgccgagttc cacttccgaa gggtggaaga tcgtgtactg catctcggtc ttcagactgt
     5581 cttcggaggg aaccaactcg tagatgctca taccatcgag cgaccagccg tgcgaagcgg
     5641 cgacggcctt gatctcttct ttggtttccg agagcgtgac gtagagcacc ggttcgccct
     5701 gcgccgcgcc ttcgagcaga aacttgagcg caagcgtggt cttgcccgtg cccggatcgc
     5761 cctcgatcag gtacaggtga ttgggcggaa agccgccctc cagaatactg tcgaaaccga
     5821 cgctccccgt ccctagtctc gcggtcacgg cggaagtctc cgcgcgcgaa ttgtcagcag
     5881 cattgtctct tgtgttcact aaggctcccg ctagtttgat aatctgatag tctgatagtc
     5941 ttcgcacgcg atatgcgcgc cggagagagc gcatttataa atggcttgag cgcatgatag
     6001 ggtagcacgg ctccgccgca aactgttaca tgttgacacc ctgaacccac ggacaagcta
     6061 tgaacaggac tctcgacgta gttgaacaga tcacggaagg cgaggcgcgt ttcgagcgcg
     6121 tgcggcgcgt agcgggactc gcgctcgcgc ccgccatctt catccttctg ctgctcctgc
     6181 ctttgcccgc tctcaagccg gaggcgcaca ggctggcggc ggtgatggcg gcggtcgtcg
     6241 ttctgtgggt gacggaggct ctgcctttgc ccgtcactgc gctgctcggc gcggcggcgt
     6301 gcgtcgtgat gcgggtcgct ccggcgaggg acgttttcgc gccgtttgcc gatccgttga
     6361 tgtttctctt catcgggtcg ttcatcatcg cgcgggcgat ctttttgcac cggctggacc
     6421 ggcggctcgc cttcggcgtg ctctcgatca agtgggtggg ggcgcgtccc ggcaggattc
     6481 tcgtggcctt cggcgcggtg acggccttca tctccgcgtg gatttccaac acggcgacaa
     6541 ccgcgatgat gttcgctatc ggcatggcga tcctcgcctt cctcttcgac aacgagcgcg
     6601 agggcggcag cccgatcaac cgccgctacg cgaccggctt gatgctgatg accgcttttg
     6661 ccgcctccat cggcggactc gccacgccca tcggcacgcc gcccaacgtc atcggcctcg
     6721 gcttcatacg ccaactcgtc ggcatcgagt ttcccttctt caagtgggcg atgatcggcg
     6781 tgcccgtggt catcattctc tttctctatc tcagttttta tctgaacgcg ttgtgccgcg
     6841 cgggcgtgcg cgagatcgaa ggcagccgcg agatgctgct gcgcgagcgt tcgcgtctgg
     6901 gcgcgtggac gcgcgggcag aaatcaacgc tcgtcgcttt tctcgccacc gtcgcgctct
     6961 ggatcgtgcc cggcctcatc gcgctcttcg cgggagatca aagtgcgatc tatcaggcgg
     7021 tgaaccggcg cgtgccggaa gcggtggcgg cggtggtggg cgcgtcgctg ttgtttctct
     7081 tgccgggcga cgggcgcggc gagcgcgcga tcacctggga cgaggcggtg aagattgact
     7141 ggggcgtagt gctcctctat ggcggcggct tcgcgctcgg cgtgctctcg tttcagacgg
     7201 ggctggccga agccatcggc tacggcctga cgaagcacct gcccattagc ggcggcctct
     7261 cgcttctctt cgtttcgacg ctcgtggcga cgctcacctc ggaggcgact tcaaacacgg
     7321 cttcggccaa catggtcgtg cccgtcgtta tagccatctc gcgcgcggcg ggcgcagacc
     7381 cgctcgaacc cgcactcggc gcgacgatgg gcgcgagcct cggcttcatg ctgcccgtct
     7441 ccacgccctg caacgccatc gtctatggtt cgggctacat cccgctcggt cgcatgattc
     7501 gctacggcct gctgctcgat gtggtcggcg ttgtcgtcat cgtcgcggtc gtccggctcc
     7561 tcgtgccgtt cctccgttga ggactttaaa ctctgaaacc ggcggacagc ccttcaaccc
     7621 tttccggctt cctttgcgtc tccggcgtga aaactacaac tcacgcagag aagcaaagag
     7681 attagagaga agtcttgcca gcggctctga aagtttggat agttggatga acttatggaa
     7741 cgaatcgtct ttctggatcg caacacgctg agggcgaaaa tcaggccgcc cgccttcgcg
     7801 cacgagtggc gcgagtatgg cgcgacgcgc gcggaggaag tggttgagcg tcttgaagcg
     7861 gcgagcatcg ccatcgtcaa caaggtcgag atcagagacg cgcacctcgc gcagttgccc
     7921 gcgcttcgtc tgatcgcggt cgcggcgacg ggcacggata acattgacct caagagttgc
     7981 cgcgagcgcg gcgtcgccgt ctcgaacgtg cgcggctacg cggcgggcac gctcccggag
     8041 cacgtcttca tgctgatgct cgcgctccgg cgcaatctcg tcagctatcg aacggacgtg
     8101 cgcgccggac gctggcagcg cgcagaaggt ttctgcctgc tcgaccaccc gatccgcgat
     8161 ctgcacgaaa gcacgctcgg catcatcggt tacggcgcgc tcggtcgtgc ggtcgaacaa
     8221 ctcgcgcgcg ccttccgtat gcacacgctc atcgccgaac acaaaggcgc acagcacacg
     8281 cgcgccgggc gcaccccttt tgcagaggtc ttgcgcacga gcgacatcgt tacgctgcac
     8341 acgccgctca cggcagagac acgccatctc atcggcgcgc cggaactcgc tttaatgaaa
     8401 ccgtcggcct tgttgatcaa ctgcgcgcgg ggcgggatat tggacgaggc cgcgctcgtc
     8461 gaagcgttgc aggcgggcgt catcgcgggg gcgggcgtgg atgtcttgag ccgcgaaccg
     8521 ccgcgcgagg gcaacccgct cctcgaactc gacttgccta acttgatcgt cacgccccac
     8581 gtcgcatggg ccagcctcga agcgatgcag acgctcgccg atcaactgat agagaacatc
     8641 gaagcgttta taaaacagcg acgagtgacg agtggcgagt gacaggtggc gagataaaaa
     8701 aagtaaatcg tgaatcgtca atcgtgaatg gaagaaagca ggtcttctct attcatgact
     8761 gacgattcac gattcaggtc ttccctaact tgtcacgcgt ccctattgtt cgtcgtgatg
     8821 ttcggcggtg tagaggcgga gaatttcctg tccctgacgc gagcgcgggt cgatggtgcg
     8881 gaggtgcttg cgcccctgca tctcccagag cgcggtcacc tgcccgtctt tttcgacggc
     8941 gtgataggtg atctcttccg tgctttcggg agcggcggcc tctcctgcgg gcgccgctcc
     9001 gcttgcttcg ctgccgccgg tttgtccgtc cggcgtagct tccgccgcgc ctaatgcgtt
     9061 ctcgttttct gccatagtcg ttcctctcaa aaattgatct taattagctt gtttgttgtg
     9121 cgcgaattgt ggcacagacc gagcgggcgt gtccaccgct acacaaagca aaggatgagg
     9181 gatgagggat gagggatgaa tcggaaaagc actcaactcg cttgaatgct cttcttaatt
     9241 catcccttat ccctttttca atcgttcgcc ggggagaaga aacagaagag cagcgtgacg
     9301 gattgacctt cgggcaacgt ctcgcgccag tgcgtgaact ccgagccgcg atagagcacg
     9361 gcgtcgccga tctccagacg cacttcctcc gtgcgccctt caacttccag attgatcggc
     9421 cacgcgcttg ctctctccaa ctccggttcg gcatccacga ctagcgagag attccagacg
     9481 tactgtggct gatccttgtg ccgacgcaaa aacgcacccg gttgatagac cgaaacctga
     9541 caatgcgaag gccggacggg cgcgggcgtg atgcgattca cgagcgcgtt gatctgttgt
     9601 tgcacgaagc gcgaactctc ttcgtcgtag gtcgagcggc ggttgtgcgc gtaatgtttg
     9661 ttgctgctcg ccttgagatg cccgcgcgcg tcgagcgcgc gaaagtatgt gcgcaaggcc
     9721 gcgagttgga gcgggtggat gatcttgcgg agcacagcat gttgccgggt ttgcagacag
     9781 gagcgtaact cctcgcaggc gcgctgccag cgtttcgcac gctcttccgc ttcgccgggc
     9841 gcgagcagga tttgcgctga cgcgaagagc cgcgccgtct ccgtttcgag ttcggagggc
     9901 gcgctcgcgc ccgcttcgag tcggcggacg agttccgctt gcgcggggct gagccagtag
     9961 ggagcgagga cttgtgtgcc cgcgtcctca acccagagca gcggtcgctc cggcgaaaaa
    10021 cggtcgagaa acggaaggcg cgcggcaaag cgagcgggca actcgtcgcc ggtttgcagg
    10081 gcgatgcgtg aattgagtgt gagcgattcg agcggcgtcg tttccgcaca gccttcgcgc
    10141 tcgacacgtt cgcgataagg gatcaggcgg cgagacgaat catcgagcgg gcacgcgaag
    10201 tagatttcat cggaagcaaa attttcacca acgtcgtcgc cgctcgcagc aaccgtgctc
    10261 gcttcaaccg tgactgcgtc gtctgccgtc tgcgccgact cgtcgcccgc cgcgcccgtc
    10321 gcacgttcgc gcgccgctgc tgctgcaacg tcgcggagcg cgccgccttc gtagagacga
    10381 aagagcgtgg caatggccgc cacgcggtct gcggatgcga cgcgtgcgcc gagttgtttg
    10441 atcgcggcgg cgaccgtgag cggcgcggag aacatttcga ggatcgagag cgcgtgcaga
    10501 ccgcacttga tcaacttgcc ctccacagac acctgcactt cgttgctcga attgatcgag
    10561 agacgaagat aggggcaacg cttgaggacg gtttcggggt tgagtctcat tcagctctgt
    10621 tcgtcgagat tgcgcacgag gttaacgtag tcgccacggt cgaagccgtt ggcgcggaag
    10681 tagtctatga ggtcgccgtc gttccagttg acgatggtgt tgacaaccga gacgttggcg
    10741 cggcggaaac tctcacagag cgcgtccatg agcgcgctgc tcacgccccg gttgtgatag
    10801 gacggatcga cgccgatcac ttcgagccag cccgcgagct tcgcgccgaa ctcccagccg
    10861 cgcacgtcgc ccatcacgaa gccgacgacg cgcccgccat gctccgcgac gagacaggtc
    10921 gtaggcgagc gaccgatgta gtcggcggcc ttcgtctcgt agtaagcggc gtggggcttg
    10981 cccgtgatct tttcgttgat gcgcagcagt tcgtctatgt cgcgcacggt catgcggcgc
    11041 actttgatgt ctttcatacg tctggctcga aacgccctgt gccgccgcac tccgccgaag
    11101 acgcgcgtgg cgaccttgcg tacgaggcgc ggtttgaacg aacgccggat gatgttgcga
    11161 tacgtttcgt ggccggagac ggcatcgaag agcgcatcgt acatcaacgg gtcaactttg
    11221 ccgacatcga tcatcgggtc gagaaagacg cgcgcgcccg cgagcatcag gcgacggacg
    11281 gcgacgccgt aagcgtaatc gccgaagaga tcgcggcacg cgcgcgcgta gcgtgcgccc
    11341 gcctcgcgcg agacgccgtg ttccagcatc gtctccgccg cacgcagacc cgtgatgatc
    11401 gccgtcgaaa tacctttgcc tttgaagggg cgcagccacc ccgtcgcgtc gccgacgagc
    11461 gcgtagcgat ggccgtatgc gccgcgcgcg ggcgcggaag ggaagcggcc ttcgtaatag
    11521 ttgatcgcgc gttcatcgaa agcggggagc aatgctcgca cttccggtag ttgtaaaaac
    11581 tggtcgaggt cggacgaaga gacgcgttcg cccgcgatgt tgatgaggat gtgatcgcct
    11641 ttcggcgtga ccgcaccgaa ctctatgcgc gggcagcgcg gcggcaggag aaacgcgtgg
    11701 acgatgtggc cgagcttctc gccctgaaag ccgtgccccg cctcgatggt cgtcaggtaa
    11761 ctcttcaacc acttcgaagg gcgcacgaag ccacgcgcgc ccctgcctct ccccgtcacc
    11821 tcttcaaaga ccgagagcat cgcctcgtcc aacccgaacg cgccgaccac cgcatcggcg
    11881 cgctcgtaat gcgattcgct atagacgcgc acctcgtcga gttcgccttc gcgcacaaac
    11941 tcaacgcccg tcacgcgcga tccgacgact tccgctccga gcgcacgcgc ttgatctaac
    12001 aggaagcggt cgaactccgc gcgttcgacg gcgtaagtcg cctgcccgtg tccggcctcg
    12061 gaagcgtgtg cgccgtgccc catgaggagc acgttttcgc gtgcgccgtg caggcggtag
    12121 ccgaaaatct gtcgcttgat gagcgtgcgc ggaagatgca cgtcgaggtc gcgcgccagc
    12181 acttcttcga tgggcggcga gagcacgccc acgcattgat tggcgtggac gttgaaatcc
    12241 ttgccctcga agatgagcac gcgcaggcgt agagaacgcg cctgtgcgcc ctgcaacaac
    12301 ttgatggcgc acgccgcccc gcccggcccc ccgccgataa ttacgaccgt cgaaccgtcc
    12361 tgtaaacggc aatcacgcaa aaagtttttc cttgaaagtt tttccttgcg aggtgcgctt
    12421 gctgtccgaa gtgcgtctgc cgccgctgcg tcgcgcccga aaacagggac gccatcttat
    12481 cgcgtgcgac gcgggcgcgg aaatttacct gccagtctat tcgccgtatc ttccgcgcgc
    12541 ctgatacgtc ccaaactgtg agcaaacgca cccgctgcac tatatcgaaa gtttacccgc
    12601 gcgtaggact tttggccgat gcggcgcaca cgccgccttc gttatactcc gtgctcctaa
    12661 gtttaggaac attaggccaa cagacattca accaa
//
LOCUS       DASWNS010000360         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_40209, whole genome shotgun sequence.
ACCESSION   DASWNS010000360 DASWNS010000000
VERSION     DASWNS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40209"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VGB76_12690"
     CDS             <1..315
                     /locus_tag="VGB76_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0099796.1"
                     /translation="RLGSPLVMLSACETGLGRERRGEGVIGLTRAFMYAGAPTVGVSL
                     WSVGDQSTAVLMPEFYRRLLAGGGSAPSAAMRASQLSMIAGKKYSAPFYWSPFVLVGD
                     WR"
     gene            579..1484
                     /locus_tag="VGB76_12695"
     CDS             579..1484
                     /locus_tag="VGB76_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099797.1"
                     /translation="MAKMLMSASRALVAAVLCFVVLGAGAHAAHAQQQPSAQKNPPKK
                     KKLPPGAKGFEQFANRDASDKLVTGGATRSGCLTYEEMLLCGAHQFEEGKIKEAVDLF
                     TRASALKPEQFRPQYSLGQIYESQGKYREATAAYKRAAALKIDEGMGDTQIDLMSASF
                     NLANVYALMNEHAQAISTLREVIRRLPEAYTPHYNLGLSLAALGKHREAVNAFRDATR
                     IKADYADAYYNMGLAHTKLEEWPQAVEAFRKALEHNPDYAQARYNLGLVYYLLDDQRS
                     LAAEVEALQKTKPELARELAKLNGK"
BASE COUNT          397 a          516 c          554 g          318 t
ORIGIN      
        1 cggctcggct cgccgctcgt gatgctgtcg gcctgcgaga cgggactcgg gcgcgagcgg
       61 cgcggcgaag gcgtcatcgg gttgacgcgc gccttcatgt acgcgggcgc gcccaccgtc
      121 ggcgttagcc tctggtctgt cggcgatcaa tcaaccgccg tcttgatgcc ggagttttac
      181 aggcgtctgc tcgcgggcgg gggaagcgcg ccctccgccg cgatgcgcgc ctcgcaactc
      241 tccatgatcg cgggcaagaa atacagcgcg cctttctact ggtcgccctt cgttctcgta
      301 ggcgattggc gatagccgca gggcgggtgc agcgacgaag acgaaaacgc tcgatagcgc
      361 atcgtgaata tttcgagaga tagagtttcg gagtaaacta aactacgctg tgaaagcatc
      421 tcaggccgtg cgcgtgaagg aaactgcggc gcactttcgg agtcgaagat tacgggcgca
      481 cctcgcgcaa ttgagataga attgccgcct ctttgtgctc tcccggacga gcctgccgga
      541 tgagaaatca ttcttcaatg atgagagaag gagaagaaat ggcgaaaatg ttgatgtcag
      601 cgagccgcgc tttggtcgcc gccgtgttgt gcttcgtcgt gttaggcgcg ggagcgcacg
      661 cggcgcacgc acagcagcag ccgtccgcgc aaaagaatcc cccgaagaag aagaagttgc
      721 cgccgggcgc gaagggcttc gagcagtttg ccaatcgaga cgcctcggac aagttggtga
      781 cgggcggagc gacgcgcagc gggtgtctga cctacgaaga gatgctcctc tgcggcgcac
      841 atcagttcga ggagggaaag attaaagagg ctgtcgatct gttcacgcga gccagcgcct
      901 tgaagcccga acagttccga ccgcaataca gcctcggcca gatttacgag tcgcagggga
      961 aatacagaga ggccacagcc gcctacaagc gcgccgccgc cctcaagatt gacgagggca
     1021 tgggcgatac gcagatcgat ctgatgagcg cgtccttcaa cctcgccaac gtctatgcgt
     1081 tgatgaacga gcacgcacag gccattagca ccttgcggga agtgatccgc cgcctgcccg
     1141 aagcctacac gccgcactac aatctcggac tctcgctcgc cgcgctcggt aaacaccgcg
     1201 aagccgtcaa cgcgttcagg gacgcgacga ggatcaaggc cgactacgcg gacgcttact
     1261 acaacatggg tctggctcac accaaactcg aagagtggcc gcaggccgtc gaagctttca
     1321 ggaaagcgct cgaacacaat cccgactacg cgcaggcgcg ctacaatctc ggactcgtct
     1381 attacctgtt ggacgatcag agatcgttgg cagccgaagt ggaagccctg caaaagacga
     1441 agcccgaact ggcgagggaa ctcgccaagc taaacggcaa atagcggctt cgtcaaactc
     1501 gaattaacgg cgcgctgcgc tttacggcag cagccacacc cacatcgtcc ctgcccggtg
     1561 aaagtctgaa atgatttcgc cggacacggg cgatgttgtt gttaccggcg cgagccgtgc
     1621 gtcaatcgcg gggcgaattt tcggagtggc ggggaggttg cgccgtgggg tatattcgac
     1681 ggcatcgaga ttcccccgtt agttgtagcg acttaatgaa atcatagaag aggaacgcaa
     1741 atgagaaaca gagcattgcg cgcggtgcat gcccttgccc tccat
//
LOCUS       DASWNS010000361         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_118889, whole genome shotgun sequence.
ACCESSION   DASWNS010000361 DASWNS010000000
VERSION     DASWNS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118889"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VGB76_12700"
     CDS             complement(<1..1041)
                     /locus_tag="VGB76_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099798.1"
                     /translation="MNRETRGGEESGIANLSDARARAQAARQSLERELEEVRLLLDQG
                     LTSEVLQRTTSLMRASRQDPSLLARARCLLSSALEMQGRYRESLEAVQMYEQPEERAK
                     CDPATSIYLGVQLGLAYNYTGDHPKAIALLNGALREATAHGGGAQVGSVYVALARVYR
                     SINEYTIARDHAQKALEHFRGTGDWRGLAEANFGIALAEVYEGDYEPALEHFGQALKL
                     VGDHPAPYLLGKLYTNMAGVCWFLKRPHDGIKYLETAISYYERTEHKANAADGYNNLG
                     IHLVLVGEWNRAQEALERALTLASETDKRGEQTMILDSLGELLMLRGELEEAGKYLER
                     AVELSELHGNKWY"
     gene            complement(1263..2189)
                     /locus_tag="VGB76_12705"
     CDS             complement(1263..2189)
                     /locus_tag="VGB76_12705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0099799.1"
                     /translation="MSRTPDSAVMPSSSPISSPRVGSKAHAGRPASLQLLVKGEEAEA
                     LKFLAAHPVRNVIMLSLIRDNGFESGLNRGNFYACRDARGELTGVALIGHHVLFAGRC
                     AETIGAFAKRAREHGDAHVILGDRESVGHFWSGYAQAGESPQLVLRELLLEQKWPVAV
                     REFVPGLRLAVAADIEQVAAAHAQLAFDESRINPLETDRVGFLSRIQRRVEQGRVWVW
                     IEGDRLIFKADIFSHTPEVIYLEGVYVNPSERGKGYGLRCLSQLSRELLARAKSLCLL
                     VNEENEQARAFYQRAGYKLHSYYDTIYLSPHR"
BASE COUNT          483 a          738 c          704 g          467 t
ORIGIN      
        1 gtaccactta ttgccgtgca gctccgacag ttcgacggcg cgttccagat attttccggc
       61 ctcttccagt tcgccgcgca gcatcaacag ttcgccgagc gaatcgagga tcattgtctg
      121 ctcgccgcgt ttgtccgtct cggaggcgag cgtgagcgca cgctccagag cctcctgcgc
      181 gcgattccat tcgccgacga gaacgaggtg gatgccgaga ttgttgtagc cgtcggcggc
      241 gttggccttg tgctccgtgc gctcgtaata actgatcgcc gtctctaaat atttgatgcc
      301 gtcgtgcggt cgtttgagaa accagcagac gcccgccatg ttcgtataaa gtttgccgag
      361 caggtaaggc gcgggatggt cgcccaccag tttcaaggct tgcccgaagt gttcgagcgc
      421 aggctcgtag tcgccttcat aaacttccgc gagcgcgatg ccgaagttgg cttcggccag
      481 accgcgccag tcgcccgtgc cgcgaaaatg ttcgagcgcc ttctgcgcat gatctcgcgc
      541 gatggtgtac tcgttgatac tgcgatagac gcgtgcgagc gcgacataga ccgagccgac
      601 ctgcgcgccg ccgccgtgcg ccgtcgcttc acgcaacgcg ccgttcaaga gtgcgatagc
      661 tttgggatgg tcgcccgtgt agttgtaggc gaggccgagt tggacgccga gatagatgga
      721 ggtggccggg tcgcacttcg cacgctcttc cggctgttcg tacatctgca cggcttcgag
      781 cgactcacgg taacgtccct gcatctccag agccgaagag agcaggcagc gcgcacgcgc
      841 gagcagcgaa gggtcttgcc gagaggcgcg catcagagaa gttgttcgct gcaacacctc
      901 ggaagtaaga ccttgatcga ggagcaggcg cacctcttcc agttcgcgtt cgagcgactg
      961 gcgtgcggcc tgcgcacggg cgcgtgcatc gctcaagttg gcgatgccgg actcttcccc
     1021 cccccgtgtc tccctgttca tttcccggcg gcctcccctg gaatgatctc tgccacgaag
     1081 cacaaaggca ggtcaatcgt cgctcatgtt ggtcaacacg cgtctctcgc ccacacgaag
     1141 acagagaagg tgtcctggtg actctgatta attgttgggg gtaaatatag tgggagagat
     1201 gccaaatcac aaaccgcagg ttccggcgcg tctgcaagtg tggcgcaagc gtctggcacg
     1261 cttcaacgat ggggcgacag atagatcgta tcgtagtagc tatgcaactt gtaacccgcg
     1321 cgctgataga atgcgcgcgc ctgctcattc tcttcattga ccagcagaca gagagacttc
     1381 gcgcgcgcca gcagctcgcg gctcaactgc gaaaggcagc gcaaaccgta gcccttgccg
     1441 cgctccgacg gattcacata gacgccttcc agataaatca cctccggcgt atgggagaag
     1501 atgtcggctt taaagatcaa acggtcgcct tcgatccaga cccacacgcg gccttgctcc
     1561 acgcgcctct gaatacgcga aagaaagcct acgcggtcgg tttcgagcgg gttgatgcgg
     1621 ctttcatcaa aagccaactg cgcgtgcgcc gcggcaacct gctctatatc ggcagccacc
     1681 gcaagccgca aacccggcac aaactcgcgc acggcgaccg gccatttttg ctccaacaat
     1741 agttcgcgca acaccaactg cggagactcg cccgcctgcg cataaccaga ccagaaatgg
     1801 ccgaccgatt cacggtcgcc aaggatcacg tgcgcgtcgc cgtgctcacg cgcacgcttc
     1861 gcaaaggctc cgatagtctc cgcacaccgg cctgcgaaga ggacgtgatg cccgatgagt
     1921 gcgacgcccg tgagttcgcc gcgcgcatcg cggcacgcat aaaagtttcc gcgattcaga
     1981 ccgctctcga agccgttgtc gcggatcaaa ctcagcatga tcacgttccg caccggatgc
     2041 gctgcgagaa acttgagggc ttccgcttcc tcgcctttga cgagaagttg aagcgaagcg
     2101 ggacggcctg cgtgcgcctt gcttcccacg cgcgggctgg agataggcga cgacgacggc
     2161 atcacggcag agtcaggagt tcgagacata acatttgaga gatagcgcgt cagggagatt
     2221 caagataacg gagctattta gcgcgagagc aacgcgcgtc gtcagtaccc tatgtggtca
     2281 tttccggtgt caatcacgac gggcgcataa ctggtctggt cgccattcgc cagcgtcccc
     2341 agcgccctga acagcgtgtc gctcatgatc acgccgtcgc tcatgatcac gc
//
LOCUS       DASWNS010000362        17270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_40237, whole genome shotgun sequence.
ACCESSION   DASWNS010000362 DASWNS010000000
VERSION     DASWNS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17270
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40237"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3454
                     /locus_tag="VGB76_12710"
     CDS             <1..3454
                     /locus_tag="VGB76_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0099800.1"
                     /translation="TAPLIFIALKGQTQIPVNERLVLDITPVDQVAAVMLAVAAEACV
                     EEPRLVYQAATGDSNPNDMRRIVGMLGLYKRKHFDEKETGFRIVNAVASRMETRPVTT
                     SRFEKTSTPMINAAAKKASSFLDNVRPRWGGGRLTEFVDHAKKHVERLEELTHETKEA
                     FEMFRPFMVENAYIFRADNVRSLFSRIRQDEQDLLPWHPEKFDWYDYWMNVHFPGLKK
                     WIFPQLEEDMRAQPKRVYTYRDLLELFETSSKRYATRVAMRIERDGRKEQYTYRDVHE
                     LATRAAGFFASEGLRAPSRVVLLSHNAPEWGMTYFGVLKAGGTCVPIDPESTTGEIIN
                     FIRASEAFGIVLSDEMREKHDDLHERLIEEKLETTRIWRFEEVFALPPQKIEDQRLAL
                     LPSKIHPQTAASLIFTSGTTGQPKAVMLSHKNFTSMVSMLASIFDMTTKDGVLSVLPL
                     HHTFEFSTGFLTPLSRGAQITYLPELTSEELTRAIKNGYVTGMVGVPALWELLHRRIK
                     SRFNERGKLIGDTADYLIEANSWIRDHSPFNLGQIIFRPIHAGLGGRIRYLISGGSAL
                     NERVKKDFHGLGFTILEGYGLTEASPVLTVTRPQNKLISMSVGKPLPGVEVKILDPDA
                     SGIGEVVARGPNVMSGYFNNEEATRAVLVNRWLYTGDLGRLDEEGNLYLVGRSKEIIV
                     DTNGKNVYPDEIEELYFDSPYIKELSVVGLPDGIGEKVACMVVADHDYDIALSREEVR
                     RKVEEHFRDVSSSLPFYKRVKVLHFWDGELPRTATRKVKRREVVAAMQVLEESMRSVS
                     QVSTNVREDRGATWLLEVVATVANRPRATVTFDTRLADLGFDSLMYVELASAIEQAGG
                     TLRSPDTLTEVRDLRELAGVVTRQATPAEREEAERRASADARRGEDEIYVPSLVRTVG
                     NKGLDALQRIFYEKFLHMKIEGESNIPVHANFIVASNHASHLDMGLVKMSLGEHGRDM
                     VALAAADYFFDNKFKRAYMDNFTNTVPMERSGSLRKSLRQALSFLERGYNALLFPEGT
                     RSVTGELADFKPVLGFLALTARVGILPVYLHGTYEALPKGSNMIKSRDISARIGRFLS
                     AEELAEMTRGMSKSESYRLVAALVRHQVENLRDGTRHNFDAKALRKRWRAERRGTPEE
                     EAVV"
     gene            3564..4667
                     /locus_tag="VGB76_12715"
     CDS             3564..4667
                     /locus_tag="VGB76_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0099801.1"
                     /translation="MVSKKATTKARKAPAKAKAASKKDVSHALVRRVQPYTLVTGGTG
                     FLGAHLIRQLVEAGEQNVRVLATTSPAWLADLGVETVTGSVLNRADVARALDGVASVY
                     HLAGRVSRDRDDARGMYELHVEGTRVLCDAARVAGVKSIVLASSSGTIAVTDDATALP
                     NESWPPPLDIISRWPYYASKYYQERVALEHFSGAGERLVIVNPSLLLGPGDERLSSTK
                     VVLDFLARKILAVPGGGLSFVDARDAAEALRAAMKRGAHGERYLLGAANWTFTKFFGR
                     LERLTKVAAPRFSLPSKFAVSGAQALHSLYRQWKLTPPIEPGEIEMAEYFWYFDTSKA
                     ARGLGFAARDPAETLQDTVTYVRENFLGKGAFV"
     gene            complement(4761..5807)
                     /locus_tag="VGB76_12720"
     CDS             complement(4761..5807)
                     /locus_tag="VGB76_12720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0099802.1"
                     /translation="MNKKTPKVEQGAGVAGRGEAVAIAASTSVVAHHSSLPLVIVNPA
                     SAGGATGRAWPRLASDLRTHFGAFNCAFTERAGDARLIAGREAGQGRNLIVACGGDGT
                     ISEVANGILESGADAELGILPSGTGGDFKRTLKIPARVPDAAAALRSGRSVCLDVGRV
                     EFRDRAGAPASRYFLNVASCGMGGEVIRRVEENSSGWLTSASRRIGGGQLSYALASLQ
                     TTVSFNNRTLRLQLDDHPEFRLVVANLCVANARYFGGGMKIAPEAKLNDGWLDVVAVG
                     DLGTLEILTNVYKLYLGTHLGMQKVRHALARRIRVSAADAGEQVLIEIDGELSGTLPA
                     TFEILPQRLRVRCP"
     gene            complement(5804..6721)
                     /locus_tag="VGB76_12725"
     CDS             complement(5804..6721)
                     /locus_tag="VGB76_12725"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0099803.1"
                     /translation="MSSTELEPPPREIEPQTAHAPLAVLAEPTPEELAVLAPMERVAF
                     RVTRRMNRGGWKRFWTFCQRTLGMSWIRLCTYNLMRVHGIEHVEAVSHERPILLVSNH
                     RSFFDMYTVAAELFRRTSWEKRLFFPVRGRFFYESPLGMFVNMVMGWWSMYPPFFAAG
                     SNPIPEKRDFDKYSMRVLVDLCQQGRGHLIGFHPEGTRNKNPDPYSFLPAKPGVGRLI
                     KDATPQVIPVFVAGLNNDLPRQVLGNWTGGELIRVHFGAALDYTEFLPMRDGIRTYKE
                     IAEFVMSKIAELGEQDRAMYGNKGIGDEG"
     gene            complement(6811..10368)
                     /gene="metH"
                     /locus_tag="VGB76_12730"
     CDS             complement(6811..10368)
                     /gene="metH"
                     /locus_tag="VGB76_12730"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY0099804.1"
                     /translation="MKDFLQTLQDRIVVFDGAMGTNLQTQNLTIDDYGGAQFEGCPEY
                     LLISRPAAVERVHAAFFEAGCDVVETNSFGAIPYVLDEYGIGHMAYDLNVRAAQLARR
                     IAADFSNAERPRWVAGSMGPGTKSPTLGHIAFRDIKAAYYEQARGLIDGGVDLLIVET
                     CQDLLQTKATLSAIFEYFEQAKRRVPVITQVTIELFGTMLPGTEISAALTALEPFPID
                     VIGMNCGTGPRNMTESIRYLCENATLPVSVLPNAGLPSVKDGAMHYDETPESFTAQVA
                     HFVKDFGVNVVGGCCGTTPEHLRMLVEAMQGVQPKRRTGSVLPAASSIYSQQPYVQQN
                     SFLIVGERVNASGSKKMRDLLNAEDWDGLVSLAREQEREGAHVLDVNVDFVGRDGVKD
                     MHELASRLVTNIRLPLMFDSTEWEKMEAGLEHAGGKCILNSTNYEDGEPRFTKVIELA
                     KRFGAGVVIGTIDEEGMARTAEGKFRIARRAYEQATAQLGYPAHDIFFDPLALPISTG
                     IEEDRRNAAETIEGIRRIKRDLPGCFTILGVSNISFGLNPASRVVLNSVFLHEAIEAG
                     LDAAIVNASKIVPLNRIGEREREVARQLIYDERKFDGEICTYDPLGEFTKLFEGVTTK
                     KVHVDDAALPVEESLKRHIIDGEKQGLETQLKRALESYPALDIINNILLDGMKVVGDL
                     FGSGQMQLPFVLQSAEVMKAAVRFLEPFMEKKGGATAKGKMILATVKGDVHDIGKNLV
                     DIILTNNGYSVVNLGIKQTIEQILAAYTEQGADAIGMSGLLVKSTLIMKENLEILNER
                     EIKVPVVLGGAALTRRYVEDDLKAIYQGTLFYAKDAFDGLHTMDLLTGVKSDDAGAQA
                     AKGKAAAATAGMAGADAEAATDDAEDLIGEEAKLGIRKPARPRGVTKVAGDTTHTRRS
                     DVRADVPIPRAPFYGSRVVDEIPLEEVFAFVNETALFKGQWQFKQGRRSAEDYRALVA
                     EKVRPVYDELKERSKREALLVPRVVYGYFPCQSAGNDLIIYEEDERTERQRFTFPRQP
                     EGKRLCLADYFAAQDSGRVDTVAFHLVTMGRRASEYAHELFKSDNYTDYLYFHGLSVE
                     SAEALAELWHKRIREELGIAERDAKEITKLFHQGYQGSRFSFGYPACPNLEDQTKLFE
                     LLDPSRIDVALTEEFQLEPEQSTSAIIVHHEEAKYFSID"
     regulatory      complement(10499..10574)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            10758..11252
                     /gene="coaD"
                     /locus_tag="VGB76_12735"
     CDS             10758..11252
                     /gene="coaD"
                     /locus_tag="VGB76_12735"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY0099805.1"
                     /translation="MRRAIYPGSFDPVTNGHLDIIARGCKLFDEIVIAVVVNPEKRPL
                     FTLDERREMLSEVLAEIESGSCRLMVDNFQGLLMQYAVEREADAVVRGIRAFSDYEYE
                     LQMALMNRRLEPRIETVFMMPAEAYSYVSSRLVKEVYQLGGTIKGLVPESVEARMQKK
                     FNKS"
     gene            11374..11721
                     /locus_tag="VGB76_12740"
     CDS             11374..11721
                     /locus_tag="VGB76_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099806.1"
                     /translation="MPRGDKSAYTDKQKRQAEHIEESYEQRGASEDEAERRAWATVNK
                     ISGGGKKSGSGRGKAENKAPSKKGGERGGKAAAARPASARSASAKKAAGTRARKGSAK
                     KGGAKKGGAKKGS"
     gene            11750..12316
                     /locus_tag="VGB76_12745"
     CDS             11750..12316
                     /locus_tag="VGB76_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099807.1"
                     /translation="MKIGTHCLSFVVAACVLSGGQWVFSQTVNNEPRFVTLQARRKTD
                     VQDNYVEAAFSFEHGVNGKEALRITHNDWDLLFGNSFVADTFDVTMVTDDCSRIKDLG
                     EFGWYDSFKVPVLPAHDKPTREPSVRAVVGHMYVVHTKDRETDLYALFRVEALEPGTS
                     VTISWKRVPPPENEPQDTNSPPAGRENF"
     gene            12322..13527
                     /locus_tag="VGB76_12750"
     CDS             12322..13527
                     /locus_tag="VGB76_12750"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0099808.1"
                     /translation="MTKLALETFPLSERVAPMEASSTLAAMQKALALRAAGVDVVDLG
                     AGEPDFDTPEHIKRAAAEAMRAGATKYTATGGTRALQEAIVNYYEREFGVRYDTSEVM
                     ATAGGKQAIFNAVVTLANAGDEVLIAKPYWVTFPEIAKFAGAKPVFIETEETGFQLTA
                     EQVRRAITPRTKLIIINSPSNPSGRVIPPAEFRKIMEVVAEHNIYAVSDECYLRFVYP
                     PAEVFSASSLAPELRARLCIAGSFSKTYAMTGWRMGYALAPRDWTKAMLLVQGHSTSN
                     PNAIAQQAAVEALNSSQECVAGMLAEYGARREWLVRAMGDIPGFCCFAPEGAFYAFPN
                     VRGCLQGKVKTSGEFAEKLLNEEQTVVTDGAGFGASGYIRISYATSLDRLEEGIRRIR
                     RVADRCAAR"
     gene            complement(13651..14985)
                     /locus_tag="VGB76_12755"
     CDS             complement(13651..14985)
                     /locus_tag="VGB76_12755"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0099809.1"
                     /translation="MQFEFCPVDGTPLSNGSRAANTDTATISNSAHERTASTDPVNDP
                     AQTDATTATIPAAQIPASTGSPDEARAMIPRGEYHLTFLEDEGLIRRLSKEVREVGHD
                     AELTWPEFKRDPGGFVKRGATAYSKASWRVLSQRNVALGIMSALVLVITAGVLVALLL
                     SLQDKHVAALDAVREDLEFQGIVTDIPEEQKKPDEGTAGTAKGKGGGSKPKQNKPGGG
                     GGGGRQEEKPASFGKLPPASLEVPQVRAPDPRPPTIKNPSLPVPASIVADPTLFPPDN
                     RQIAYGDPKSRSTETSSGPGSGNGIGTGTGGGVGSGEGGGVGPGRGGNTGGGDRHDGG
                     GGPGGGGGGGPDYSKPFSQRDVTRKANITSKPEPLYTEEARKNQVTGTVRLRLILGAS
                     GSVSGITPLTRLPDGLTEKAIEAARRISFTPAEKDGRKVSQYVTIEYNFNIY"
     gene            15313..16740
                     /locus_tag="VGB76_12760"
     CDS             15313..16740
                     /locus_tag="VGB76_12760"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0099810.1"
                     /translation="MKRQFLQLKTQAARTTSVAVLLLVSLCLHAPAHVAAQTSGTPAP
                     RREQLLNGLRLLLLHRPGDSQVALKLRINSGAAFDLAGKEGLTALLSDALFPDPGTRQ
                     YVTEELDGRLEVNTTYDSIEISLSGKASDFERLAEVLRNALMQMRLAPEDVRRLLDER
                     LKAAREANAKPDLLADRAIAARLYGTYPYGRTLRGTPESLARIDRFDLMLARDRFLIP
                     NNSTLTLIGGVEAPRAMRVFRQFLGAWRKGETIAPYTFRQPDAPDARTLIVNLPGTNE
                     AEIRLAVRGLSRTDKDRAATHIIAALARERWAAALKEFQPRSTFARHDSHYVTGELIL
                     GATVPAGVAAQTLEAARTLVKNLAVAPVSVAELDKARRTALADFEARKAQPDALANEW
                     LDAIFYKSTLAEEELALNALTPADVQRVAARLFAEAKLASVVVGDAAQLRNELARLSG
                     GIEEAGTKPATPPASAAEPKTPGRP"
     gene            16801..>17270
                     /locus_tag="VGB76_12765"
     CDS             16801..>17270
                     /locus_tag="VGB76_12765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0099811.1"
                     /translation="MSTPTSLNETERRQEHARRVNSMFAGIAGRYDLLNHLLSANIDR
                     RWRRLVADELRPALVAGAQTLDVACGTGDLALTLAEAGQARVTGLDFCRPMLEVASKK
                     AAQRQRGIPFIEADALRLPFAAETFDVVTIAFGLRNLASVEGGLRELFRVLRPGG"
BASE COUNT         3327 a         5283 c         5324 g         3336 t
ORIGIN      
        1 gaccgcgccg ctcatcttca tcgcgctcaa agggcagacg caaattcccg tcaacgagcg
       61 tctggtgctg gacatcacgc cggttgatca agtcgccgca gtgatgctcg ccgtcgccgc
      121 cgaagcgtgc gtcgaagagc cgcgcctcgt ctatcaggcc gccaccggcg acagtaatcc
      181 gaacgatatg cgccgcatcg tcggcatgct cggcctctac aagcgcaagc acttcgacga
      241 aaaagagacg ggcttccgca tcgtgaacgc cgtcgcctcg cgcatggaga cgcgtcccgt
      301 tacgacttcg cgtttcgaga agacttcgac gcccatgatc aacgcggcgg cgaaaaaagc
      361 ctcttcgttt ctcgacaacg tgcgcccgcg ctggggcggc ggtcgcctca cggagtttgt
      421 cgatcacgcg aagaagcacg tcgaacgcct cgaagagttg acgcacgaga cgaaggaagc
      481 cttcgagatg tttcgcccgt tcatggtcga gaacgcctac atcttccgcg ccgacaacgt
      541 gcgctcactc ttctcgcgta tccgccaaga cgaacaagac ctcttgccct ggcatcctga
      601 aaagttcgac tggtacgact actggatgaa cgtccatttc cccggcctca agaaatggat
      661 cttcccgcaa ctcgaagagg atatgcgcgc acagccgaag cgcgtctaca cctatcgcga
      721 cctgctcgaa ctcttcgaga cttcatccaa acgctacgcc acgcgtgtcg ccatgcgcat
      781 cgaacgcgac gggcgcaaag agcagtacac ctatcgagat gtgcacgaac tcgccacgcg
      841 cgccgcaggt tttttcgcca gcgagggctt acgcgcgccc agccgcgtcg tgctgctcag
      901 ccacaacgcg cccgaatggg gcatgaccta tttcggagtg ctcaaggcgg gcggaacctg
      961 cgtgcccatc gacccggaga gcacgacggg cgagatcatc aacttcattc gcgcgagcga
     1021 agcgttcggc atcgtcctga gcgacgagat gcgcgagaag cacgacgatc tccacgagcg
     1081 tctgattgaa gagaaattag agacgacgcg catctggcgt ttcgaagagg tcttcgcgct
     1141 gccgccgcag aagatcgaag accagcgact cgccctgctg ccctcgaaga ttcacccgca
     1201 gacggcggct tcgctcatct tcacttccgg cacgaccggc cagcccaaag ccgtcatgct
     1261 ctcgcacaag aatttcacga gcatggtttc gatgctcgcc tccatcttcg acatgacgac
     1321 gaaggacggc gtgctctccg tcctcccgct ccatcacacg ttcgaattct ccacgggctt
     1381 tctaacgccc ctctcgcgcg gcgcgcagat cacttacctg cccgaactga cgagcgagga
     1441 actcacgcgc gcgatcaaaa acggttacgt gacgggcatg gtcggcgtgc ccgctctgtg
     1501 ggaactcctg caccgccgca tcaagagccg tttcaacgaa cgcggcaaat tgatcggcga
     1561 cacggcggac tatttgatcg aagccaatag ttggatacgc gatcactcgc cgttcaatct
     1621 cggccagatc atcttccgcc cgattcacgc cggactcggc ggtcgcatac gctacctgat
     1681 cagcggcggc tcggccttga acgagcgcgt caaaaaagat ttccacggcc tcggcttcac
     1741 catcctcgaa ggctacgggc tgacggaagc ctcgcccgtc ctcacggtta cgcgcccgca
     1801 aaacaagttg atctcgatga gcgtgggcaa acctctgccc ggcgtcgaag tgaagattct
     1861 cgacccggac gcgagcggca tcggcgaagt cgtcgcgcgg ggaccaaacg tgatgagcgg
     1921 ctatttcaac aacgaggagg cgacgcgcgc cgtcctcgtc aatcgctggc tctacacggg
     1981 cgatttaggc cgcctcgacg aagaaggcaa tctctacctc gtcggtcgct ccaaagagat
     2041 catcgtggat acgaacggca agaacgtcta tccagatgag atcgaagaac tctatttcga
     2101 ttcgccctac atcaaggaac tgagcgtggt cgggttgccg gacggcatcg gcgaaaaggt
     2161 ggcgtgcatg gtcgtggcgg atcacgacta cgacattgcg ctctcgcgcg aagaggtgcg
     2221 ccgtaaagtc gaagagcatt tccgcgacgt gtcttcctcg ctccccttct acaagcgcgt
     2281 caaggtgctg catttctggg acggcgaact gccgcgcacg gcgacgcgca aggtcaaacg
     2341 ccgcgaagtc gtcgccgcga tgcaggtgct cgaagagagc atgcgttccg tctcgcaagt
     2401 ctcaaccaac gtgcgcgaag acagaggcgc gacgtggctg ctcgaagttg tcgcgacggt
     2461 cgccaatcgc ccgcgcgcca cggtcacctt cgacacgcgc ctcgcagatt tgggtttcga
     2521 tagtttgatg tacgtcgaac tcgcgtcggc catcgagcag gcgggcggca ctctgcgttc
     2581 gccagatact ctgaccgagg tgcgggattt gcgcgaactc gcgggcgtcg tgacgagaca
     2641 ggcgacaccc gccgaacgag aggaggccga gcggcgcgcg agcgcggatg cacggcgcgg
     2701 cgaggacgag atttacgtac cctcgctcgt acgcaccgtc ggcaacaagg gactcgacgc
     2761 gttgcagcgt atcttctacg agaagtttct gcacatgaag atcgagggcg agtcgaacat
     2821 ccccgtgcat gcgaacttca tcgtcgcatc caatcacgcg tcgcatctgg acatgggact
     2881 cgtcaagatg tcgctcggcg agcacgggcg cgatatggtt gcgctcgccg ccgcagatta
     2941 tttcttcgac aacaagttca agcgcgccta catggacaac ttcacgaaca ccgtgccgat
     3001 ggagcgcagc ggttccctgc gcaagtcgct gcggcaggcg ctctcgtttc tcgaacgcgg
     3061 ttacaacgcc ctgctcttcc ccgaaggcac gcgctccgtt acgggcgagt tggccgactt
     3121 caagccggtg ctcggcttcc tcgcgctcac agcgcgcgtc ggcatcctgc ccgtctacct
     3181 gcacggcact tacgaagcgc tgccgaaggg gtcgaacatg atcaagagcc gcgacatctc
     3241 tgcgcgcatc ggtcgcttcc tgagcgcgga ggagttggcg gagatgacgc gcggaatgtc
     3301 gaaatctgaa tcctaccgcc tcgtcgccgc gctcgtgcgt catcaggtgg agaacctgcg
     3361 cgacggcacg cgccacaact tcgacgccaa agccctgcgc aaacgctggc gcgccgaacg
     3421 acgcggcaca cccgaagaag aagcagttgt ttaagagcag tgacaagtga cgagtgacga
     3481 gcgacaggtt aaagcggcaa tccgttcttt aaacttgtct ttcatctcgt tacttgtcac
     3541 tcgtcacttg tcactgaatt tttatggtta gtaaaaaagc gacgacaaag gcgcggaagg
     3601 ctccggctaa agcgaaagcc gcgtcgaaga aggacgtgag ccatgcgctc gtgagacgcg
     3661 tgcagcctta cacgctggtg acgggcggga cgggcttcct cggcgcgcac ctgattcgcc
     3721 aactcgtgga agcgggcgag cagaacgtac gcgtgctggc aacgacttct ccggcgtggc
     3781 tcgctgatct gggcgtggag acggtgacgg gttccgtctt gaatagagcc gatgtggcgc
     3841 gcgcgttaga cggcgttgcg tccgtctatc atctggcggg gcgcgtctcg cgcgaccgcg
     3901 acgacgcgcg cggcatgtat gaactgcacg tcgaaggaac gcgcgtgctc tgcgatgcgg
     3961 cacgggtggc gggcgtgaaa tcaatcgtgc tggcttcttc gagcggcacc atcgccgtga
     4021 cggatgacgc gaccgcgctg ccgaacgaga gttggccgcc gccgctcgac atcatttcgc
     4081 gttggcctta ttacgcgagc aagtattatc aggagcgcgt ggccttggaa catttttccg
     4141 gcgcgggcga gcggctcgtg atcgtcaacc cgagtctgct gctcggcccc ggcgatgagc
     4201 gcctgagttc gacgaaagtg gtgctcgatt ttctcgcgcg aaaaattctg gccgtgccgg
     4261 gcggcggcct gagttttgtt gacgcgcgcg acgcggcgga ggctttgcgc gcggcgatga
     4321 agcgcggcgc gcatggcgaa cgttacctgc tgggcgcggc caactggact tttacaaaat
     4381 tcttcgggcg gctcgaacgc ctgacgaaag ttgccgcgcc gcgcttctcg cttccgtcaa
     4441 aatttgccgt ctccggcgcg caagctctcc actcgctcta tcgccagtgg aaacttacgc
     4501 cgcccatcga acccggcgag atcgagatgg cggaatactt ctggtatttc gacacttcga
     4561 aggccgcgcg cggcctcggg tttgcggcgc gcgacccggc ggagacgctg caagacacag
     4621 tgacttacgt gcgcgagaat ttcctcggca aaggcgcgtt cgtctaagcc gcagcaaaga
     4681 gaagatcgaa aaaagttttg cggataaagg cggcgcgcag cgtgccgcag cgacgcggag
     4741 agcaggctta cgggagaggt ttaagggcaa cggacgcgta ggcgttgcgg caaaatctcg
     4801 aacgtcgcgg gcagcgtgcc tgacagttcg ccgtcaattt cgatcagcac ttgttcaccc
     4861 gcatcagccg cactgacacg aatgcggcgc gcgagcgcat gtcgcacttt ctgcattccg
     4921 agatgcgtgc cgagatagag tttgtagacg ttggttaaga tctcaagcgt gccgaggtcg
     4981 ccgacggcta ccacgtccaa ccagccgtcg ttgagtttcg cctccggtgc gatcttcatt
     5041 ccgccaccga agtagcgcgc gttggcgaca catagattgg cgacgacgag gcgaaactcc
     5101 ggatggtcgt cgagttgcag gcgaagcgtg cggttgttga aagagacggt cgtttgcagc
     5161 gaagccagcg cataggagag ttgcccgccg ccgatgcggc gcgacgcgga ggtgagccaa
     5221 ccggatgagt tttcttccac ccggcggatg acttcgccgc ccatgccgca cgaagcgacg
     5281 ttcaaaaaat agcgactcgc gggtgcgccc gcgcgatctc gaaattcgac gcgccccacg
     5341 tccagacaaa cgcttcgccc gctgcggagc gcggcggcgg catccggcac gcgtgccgga
     5401 atcttcaagg tgcgcttgaa gtcgccgccc gtcccgctcg gtaaaatccc caactccgcg
     5461 tctgcgcccg attcgagaat cccattcgcc acttccgaga tcgtgccgtc gccgccgcag
     5521 gctacgatca aatttcttcc ctgcccggcc tcacgtccgg cgatcaggcg cgcatctccc
     5581 gcccgctccg tgaaggcgca attaaaggct ccgaaatgcg tgcgcaggtc gctcgccaga
     5641 cgcggccacg ccctgcccgt cgcgccgccc gccgacgctg ggttaacgat aactaaagga
     5701 agtgatgagt gatgcgcgac gacggatgta gacgcagcga ttgcgacggc ctcgcccctt
     5761 ccggcgacgc ctgcgccctg ttcaactttc ggggtcttct tattcatccc tcatccccaa
     5821 tgcctttgtt cccgtacatc gcgcgatcct gttcgccgag ttcggcgatc ttggacatga
     5881 cgaactccgc gatctctttg taggtgcgga tgccgtcgcg catggggaga aactccgtgt
     5941 agtcgagcgc ggctccgaag tggacgcgga tcaactcgcc gcccgtccag ttgccgagca
     6001 cctgccgggg cagatcgttg ttgagtccgg cgacgaagac ggggatgact tgcggcgtcg
     6061 cgtctttgat gaggcgtccg acgccgggct tggcgggaag aaacgagtaa gggtctggat
     6121 ttttgttgcg cgtgccttcg gggtgaaagc cgattaaatg tccacgcccc tgctggcaga
     6181 gatcgacgag cacgcgcatc gagtatttat caaagtcgcg cttctccggg atcgggttgc
     6241 tgcccgccgc gaagaagggc gggtacatag accaccagcc catcaccatg ttgacgaaca
     6301 tgccgagcgg cgactcgtag aagaagcgac cgcgcaccgg aaagaagaga cgcttctccc
     6361 aactcgtccg gcgaaagagt tccgccgcga cggtgtacat gtcgaagaac gaacgatgat
     6421 tggagacgag caggatgggt cgttcgtgcg agacggcttc gacgtgttcg atgccgtgta
     6481 cgcgcatcag gttataggtg cagagacgaa tccaactcat gccgagcgtg cgctggcaga
     6541 aagtccagaa gcgtttccac ccgccccggt tcatgcgccg cgtgacgcga aaagcgacgc
     6601 gctccatcgg cgcaagcacg gcgagttctt ccggcgtcgg ctcggcgaga accgcgagcg
     6661 gcgcgtgtgc cgtctgcggc tcgatctctc gcggcggggg ttcgagttct gtcgaagaca
     6721 tcttgataaa gtgaatcgtg aatcgtgaat cgtgaataga ctaaaacagt tttcctctat
     6781 ttacgattta cgattcacga ttcacgagtt tcagtctatc gaaaaatact tcgcctcttc
     6841 gtgatggacg atgatggcgg aggttgattg ttcgggttcg agttgaaact cctctgtgag
     6901 cgcgacatcg atacgcgagg ggtcgagcag ttcgaagagc ttcgtttgat cttcgaggtt
     6961 cgggcaggcg gggtagccga agctgaaacg cgagccttga tagccttgat ggaagagctt
     7021 cgtgatctct ttcgcgtcgc gttcggcgat gcccagctct tcgcggatgc gcttgtgcca
     7081 gagttcggcg agagcttccg cgctctcgac gctcaggccg tggaagtaga gatagtccgt
     7141 gtagttgtcc gacttgaaca actcgtgcgc atactcactc gcgcgccgcc ccatcgtcac
     7201 gagatgaaaa gcgaccgtgt ccacgcgtcc ggaatcctgc gcggcgaaat agtccgcgag
     7261 gcagagacgc ttgccttcgg gctggcgcgg gaaggtgaaa cgctgtcgct ccgtgcgttc
     7321 gtcctcttcg taaatgatca ggtcattgcc cgccgactgg caagggaaat atccatagac
     7381 gacgcgcggc acgagcaacg cttcacgctt cgagcgttcc ttcaactcgt cgtaaacagg
     7441 gcgcactttt tccgcgacga gcgcgcgata gtcttccgcc gacctgcgcc cctgtttgaa
     7501 ttgccactgg cctttgaaca gagccgtctc gttgacgaaa gcaaaaacct cttcgagcgg
     7561 aatctcgtcc accacgcgcg agccgtagaa gggagcacgc gggataggca cgtccgcacg
     7621 cacgtcggag cggcgcgtgt gcgtcgtgtc gcccgcaact tttgtcacgc cgcgcggcct
     7681 cgcaggtttg cggatgccga gtttagcttc ctctccgatc aaatcttcgg catcgtcggt
     7741 cgccgcttcc gcatccgcgc ctgccattcc ggctgtggcg gcggcagctt tgcccttcgc
     7801 tgcctgcgcg cctgcgtcgt cgctcttcac gccggtcaac aagtccatcg tgtgcaggcc
     7861 gtcgaaagcg tccttcgcgt agaagagcgt gccttgatag atcgctttga gatcgtcttc
     7921 gacgtagcga cgcgtgagag ccgcgccgcc gagcacgacc gggactttga tctcgcgctc
     7981 gttcaggatt tccagattct ccttcatgat gagcgtggat ttcacgagca ggccgctcat
     8041 gccgatggcg tccgcgccct gctcggtgta ggccgcgagg atctgttcga tggtctgctt
     8101 gatgccgaga ttgacgacgc tgtagccatt gttggtcagg atgatgtcca cgaggttctt
     8161 gccgatgtcg tgcacgtcgc ccttgacggt ggcgagaatc atcttgccct tcgccgtcgc
     8221 gccgcccttc ttctccatga acggttcgag gaaacgcacc gccgccttca tcacctccgc
     8281 tgactgcaag acgaacggca actgcatctg gccgctgccg aagagatcgc cgaccacttt
     8341 catgccgtcg agcagaatgt tgttgatgat gtcgagcgcg ggatagcttt cgagcgcgcg
     8401 tttgagttgc gtctccaagc cctgcttttc gccgtcgatg atgtggcgtt tgaggctctc
     8461 ttcgacgggc aacgccgcgt cgtcaacatg cactttcttc gtcgtcacgc cctcgaaaag
     8521 tttggtgaac tctccgagcg gatcgtaggt gcaaatctcg ccgtcgaact tgcgctcgtc
     8581 gtagatcaac tggcgcgcga cttccctctc gcgctcgccg atgcgattga gcggcacgat
     8641 cttgctcgcg ttgacgatgg ccgcgtcgag tcccgcttca atcgcttcgt gcaggaagac
     8701 ggaattgagc acgacgcgcg acgcgggatt caaaccgaaa gagatgttcg agacgccgag
     8761 gatcgtgaag caaccgggca ggtcgcgctt gatgcgacga atgccttcga tggtttcggc
     8821 ggcgttgcgg cggtcttctt cgatgcctgt cgagatgggg agcgcgagcg ggtcgaagaa
     8881 gatgtcgtgc gcgggatagc cgagttgcgc ggtcgcctgt tcgtaggcgc gacgcgcgat
     8941 cctgaacttg ccttcggcgg tgcgcgccat gccctcttcg tcaatcgtgc cgatgacgac
     9001 gcccgcgccg aaacgcttgg cgagttcgat cactttcgtg aagcgcggct cgccgtcttc
     9061 gtagttcgtc gaattgagga tgcacttgcc gcccgcgtgt tcgagtcccg cctccatctt
     9121 ctcccactcg gtcgagtcga acatgagcgg gaggcggatg ttggtgacga ggcgcgaagc
     9181 gagttcgtgc atatctttga cgccgtcgcg cccgacgaaa tcgacgttca cgtcgagcac
     9241 gtgcgcgcct tcgcgttcct gttcgcgtgc gagcgagacg aggccgtccc aatcttccgc
     9301 gttcaacagg tcgcgcatct tcttcgagcc ggaggcgttc acgcgctcgc cgacgatgag
     9361 gaaagagttc tgctgcacgt aaggctgctg gctatagatc gaagaggccg ccgggaggac
     9421 gctgcccgtg cgccgcttcg gctgaacgcc ctgcatcgct tcgaccagca tccgcaaatg
     9481 ttcgggcgtc gtgccgcagc aaccgccgac gacgttgacg ccgaagtcct tgacgaagtg
     9541 cgccacctgc gccgtgaacg actccggcgt ctcgtcgtag tgcatcgcgc cgtctttgac
     9601 cgagggcagc cccgcgttcg gcaggacgga gacggggagc gtcgcgtttt cgcaaagata
     9661 gcgaatgctc tcggtcatgt tgcgtggtcc tgtgccgcag ttcatgccga tcacgtcgat
     9721 ggggaagggt tcgagcgcgg tcagggctgc gctgatctcc gtgccgggga gcatcgtgcc
     9781 gaacagttcg atggtgacct gcgtgatgac gggcacacgc cgcttcgcct gttcgaaata
     9841 ttcgaagatg gctgagagcg tcgccttcgt ctgcaacaag tcctggcagg tttcgacgat
     9901 gagcagatca acgccgccgt cgatgaggcc gcgcgcctgt tcgtagtagg ccgctttgat
     9961 gtcgcggaag gcgatgtggc cgagcgtggg cgatttcgtt ccgggtccca tcgagcctgc
    10021 gacccagcgc gggcgttctg cgttggagaa gtctgcggcg atgcgcctgg cgagttgggc
    10081 ggcgcggacg ttcaagtcgt aggccatgtg gccgatgccg tattcgtcga ggacgtaagg
    10141 gatcgcgccg aaggagttgg tttcgaccac gtcgcagccg gcttcgaaga aagccgcgtg
    10201 cacgcgctcg accgcagcgg gacgactgat cagcaaatat tcgggacagc cttcaaactg
    10261 cgcgccgccg taatcgtcta tcgtcaaatt ctgcgtctgg aggttggtgc ccatcgcgcc
    10321 gtcgaagacg acgatgcggt cttgcaaagt ttggaggaaa tctttcatgt agttttaatt
    10381 cttctccgtt ccggcctgcg aaaattcatg gcgtaagagc caagcgcaaa agaggaagcc
    10441 ccgcgccaaa caacgcgggg ctttcgtccg ggtgactcga aatgcgggtg gtttcccgtg
    10501 aaagacttgg ccgagctgtt tagctgttta ttttacgtgg ccgcaagtcg ccttaactca
    10561 ccacgtttgt ccgtcgccgt ttattgaatc accgcccgca gggcatgtca agaaagcaga
    10621 gactggcgtt tagagattag aggtctgtga aaacgggctt cgttttaacc gtcctctgac
    10681 ctctggcctc taacctctgc tttatgatac tttcacagtc gtttttgctc ccaacttaag
    10741 accaacaacg cctaactatg cgccgagcca tttatcccgg ttcttttgat cccgtgacga
    10801 acggccatct cgacatcatc gcgcgcggct gcaagctctt cgatgagatc gtgatcgccg
    10861 tcgtggtcaa cccggagaag cgtccgctgt ttacgctcga cgagcggcgc gagatgctgt
    10921 cggaagtgct cgcggagatc gagagcggtt cgtgtcgcct gatggtggat aactttcaag
    10981 gactgctgat gcaatacgcc gtggagcgtg aagccgacgc ggtggtgcgc ggcattcgcg
    11041 ccttctccga ttacgagtac gagttgcaga tggctctgat gaaccggcgg ctcgaaccgc
    11101 gcatcgagac ggtcttcatg atgcccgcag aggcttactc ttacgtcagt tcacggctcg
    11161 tcaaagaagt ctatcaactc ggcggcacga tcaagggact cgtgcccgaa tcggtcgaag
    11221 cgcggatgca gaagaaattt aacaagagtt gatttcgcgc gaacgagcgc gcttctacgg
    11281 tgttatactg ccccctgttc caacaacaat ccgcgcgaaa ctcggactcg cttcgagcgc
    11341 gcgtgtgaga acatacagag gagggttgaa cagatgccga gaggcgacaa gagcgcgtac
    11401 accgacaagc agaagcggca ggccgagcac atagaggaaa gctacgagca gcgcggcgcg
    11461 tcggaagatg aagcggagcg gcgcgcgtgg gcaaccgtca acaagatttc cggcggcggc
    11521 aagaagagcg ggtcgggacg cggcaaggcg gagaacaaag ctccttcaaa gaaaggcggg
    11581 gagcgtggcg gcaaggctgc ggctgcgcgt ccggcctcgg cgcgctcggc ctcggcaaag
    11641 aaagccgccg ggacgcgcgc ccgaaaaggc agcgcgaaga agggcggcgc gaagaagggc
    11701 ggcgcgaaga aaggtagcta ggccggagcg aagttgagag cgaagcgata tgaaaatcgg
    11761 aacccattgc ctgtcgtttg ttgtcgccgc ctgcgtgctg tccggcgggc agtgggtgtt
    11821 tagtcagacg gtcaacaacg aacctcgctt cgtcactttg caggcgcgca ggaaaacgga
    11881 tgttcaggat aattacgtcg aggcggcgtt cagttttgaa cacggggtga acggcaagga
    11941 ggcgttgcgc atcacgcata acgattggga cttgctgttc ggaaacagct tcgtggccga
    12001 tacgttcgat gtgacgatgg taacggacga ttgcagccgg atcaaagatt tgggcgagtt
    12061 tggctggtat gattctttca aggtgcccgt gctgcccgcc cacgacaaac ctactagaga
    12121 gccgagcgtg cgcgcagtgg tcgggcacat gtacgtggtg cacaccaagg atagagagac
    12181 agacctctac gccctgttcc gcgtcgaagc gctcgaaccg gggacgagcg ttactatctc
    12241 atggaagcgc gtgccgccgc ccgagaacga accgcaggac acgaactcgc cgcctgccgg
    12301 gcgagagaac ttttagacga gatgaccaaa ctggcacttg agacattccc gctttccgag
    12361 cgcgtcgcgc cgatggaagc ctcttcgacg ctggccgcga tgcagaaggc tctcgcgctg
    12421 cgcgcggcgg gcgtggacgt ggttgatctc ggcgcgggcg aacccgactt cgacacgccc
    12481 gaacacatca aacgcgccgc cgccgaagcc atgcgcgcgg gcgcaacgaa gtatacggcg
    12541 acgggcggca cgcgcgcgct gcaagaggcc atcgtcaatt attacgagcg agaattcggc
    12601 gtccgctacg acacttcgga agtgatggcg acggcgggcg gcaagcaggc tatcttcaac
    12661 gcggtcgtca cgctcgccaa cgcgggcgac gaggttttga tcgcgaagcc ttactgggtg
    12721 acgtttccgg agatcgcgaa gttcgcgggc gcaaagcccg tctttatcga aacggaggag
    12781 acgggttttc aactcaccgc cgagcaggtg cgacgcgcca tcacgccccg cacgaaactg
    12841 atcatcatca actcgccttc gaacccttcc ggacgcgtca ttccgcccgc agagtttcgc
    12901 aagatcatgg aggtcgtcgc ggagcacaac atttatgccg tctcggacga atgctacctg
    12961 cgtttcgtct atcccccggc tgaagttttc agcgcgtcga gcctcgcgcc cgagttgcgc
    13021 gcgcgcctgt gcatcgccgg gtcgttctcc aagacttacg cgatgacggg ctggcgcatg
    13081 ggctacgcgc tcgcgccgcg cgactggacg aaggcgatgc tgctggtgca gggacattcg
    13141 acgagcaacc cgaacgccat cgcgcaacag gcggcggtcg aggcgttgaa cagttcgcag
    13201 gaatgcgtcg ccgggatgct cgccgaatac ggcgcgcggc gcgagtggct ggtgcgtgcg
    13261 atgggcgaca ttcccggctt ctgctgcttc gctcccgaag gcgcgtttta cgccttcccc
    13321 aatgtgcgcg gttgtttgca aggaaaagtt aaaacctccg gcgagtttgc ggagaaactt
    13381 ctcaacgaag agcagacggt ggtgacggac ggcgcgggct ttggcgcgag cggctacata
    13441 cgcatctctt acgcaacttc gctcgaccgt ctcgaagaag gcatccggcg cattcgccgc
    13501 gtggccgaca gatgcgctgc tcgatagcgg agacgttagc gcaagctcaa acgcgaccca
    13561 cgatcttgac acgatcttta tctcgacaaa cagaagcggc gacccacagg ttcgtgagtc
    13621 gccgcttctg cttttgctaa gggagtgcgc ttagtagatg ttgaagttgt attcgatggt
    13681 gacgtactgc gagactttgc gcccgtcctt ctcggcgggc gtgaaagaga tgcggcgtgc
    13741 cgcttcaatc gccttctccg tcaggccgtc gggcaaacgc gtcagaggcg tgataccgga
    13801 gacagagccg gacgcgccga gaatgagacg caggcggact gtgccggtga cctgattctt
    13861 gcgcgcctct tccgtgtaga gcggttccgg cttgctcgtg atgttcgcct tgcgcgtcac
    13921 gtcgcgctgg ctgaagggct tgctgtaatc ggggccgcca ccgccgccgc cgccgggacc
    13981 gccgccgccg tcgtgacggt cgccgccgcc cgtgttgccg ccgcgcccgg gaccgacgcc
    14041 gccgccttca cccgaaccga cgccgccacc cgtgcccgtg ccgatgccat taccggagcc
    14101 ggggcctgaa ctcgtctccg ttgatctgga tttcgggtcg ccgtaagcga tctgccggtt
    14161 gtccggcgga aagagcgtgg ggtctgcgac gatgctggcc ggaaccggca aactcggatt
    14221 tttaatggtc ggaggtcgcg gatcgggagc gcgcacctgc ggaacttcga gcgaagcggg
    14281 tggcagtttg ccgaacgaag cgggcttctc ttcctgtcgt ccgccgccgc cgccgccgcc
    14341 gggcttgttc tgtttcggtt tcgagccgcc gcctttgcct ttagctgtcc cggctgtccc
    14401 ttcgtccggt ttcttctgtt cttcggggat gtccgtcacg atgccttgaa actcaaggtc
    14461 ttcgcgcacc gcatcgagcg cggccacatg cttgtcctgt agacttaaga gcaacgctac
    14521 gaggacgcct gcggttatca ccaacacgag cgcggacatg attccgagcg cgacgtttcg
    14581 ctgcgagagg acgcgccagg aggctttgct gtaagcggtc gcgccgcgct tcacaaaccc
    14641 gccggggtcg cgtttgaatt ccggccacgt caactccgcg tcgtgcccga cctcgcgcac
    14701 ttccttcgag aggcggcgaa tcaacccttc gtcttccaaa aatgtcaggt gatactcgcc
    14761 gcgaggaatc atcgccctcg cctcatccgg tgagccggtc gaggcgggaa tttgtgcggc
    14821 agggatggtg gcggtcgtcg cgtccgtctg cgccggatcg tttacggggt cggtcgaagc
    14881 cgtgcgttcg tgtgcggaat tgctgatggt ggcagtgtcc gtgtttgcag ctcgggagcc
    14941 attcgacagg ggtgtgccgt ccacggggca aaattcgaac tgcacaggaa ccacttcatt
    15001 gcatgttggg cagctctttg ttgtcatacc tcgcgccttt gtctggggaa caattaactt
    15061 caaactgtag gattactcga acttaggaca catgccttcg agcatcgtga gatgctaccg
    15121 aagctgtaag agtttactac cggagataaa aatattctag tttccagccg gttgaccccc
    15181 cgcttggcag ccctctaaaa tgcacgggca tctcccgcaa ccgttttgat gcatctaaac
    15241 cgcttcgtga cgaaattatt ccaacaccgt gcagcctaca gcggtcgccc cgtccgaact
    15301 tccgagccag ttatgaaacg ccaatttctt cagcttaaaa ctcaagcggc gcgcacaact
    15361 tcagtcgccg tcctgctcct cgtctcgctc tgcctgcacg ctccggcgca cgtcgcggcg
    15421 caaacttccg gcacgcccgc accgcgccgc gaacaattgc tcaacggctt gcgcctactg
    15481 ctcctgcatc gtccgggtga ttcgcaagtc gcgctcaagc tgagaatcaa cagcggcgcg
    15541 gccttcgacc tcgcaggcaa ggagggcttg accgcgctcc tcagcgatgc cctgtttcca
    15601 gaccccggca cgcgccagta tgtcacggaa gaactggacg gacggctcga agtcaacacc
    15661 acgtatgaca gcatcgagat ttccctgagc ggcaaggcca gcgacttcga acgcctcgca
    15721 gaagtgctgc gcaacgcact catgcagatg cgcctcgccc ccgaagatgt cagacgcctg
    15781 ctggacgaac gcctcaaggc cgcgcgcgaa gcgaacgcca agcccgacct tctggccgac
    15841 cgcgccatcg ccgcccgcct ctacggcacg tacccttacg ggcgcacgct ccggggcacg
    15901 cccgaaagcc tcgcgcgaat tgatcgcttc gacctcatgc tcgcgcgcga ccgcttcctc
    15961 attcccaaca attccacgct cactttaatc ggcggcgtcg aggctccgcg agccatgcgc
    16021 gtgttccggc aatttctcgg cgcatggcgc aagggtgaga cgattgctcc ctacactttt
    16081 cgtcaacccg atgcgcccga cgcacgcacg ctcatcgtca atctccccgg cacgaacgaa
    16141 gcagagatac gactcgccgt gcgcggcctc tcccgcacag acaaagatcg cgccgccacg
    16201 cacatcatcg ccgcgcttgc gcgcgagcgt tgggcggctg cgctcaagga gtttcagcca
    16261 cgttcgactt tcgcgcgcca cgattcgcat tacgtgaccg gcgagttgat actcggcgcg
    16321 accgttccgg cgggcgtcgc cgcgcagacg ctcgaagcgg cgcgcacgct cgtcaaaaat
    16381 ctcgccgtcg cgcccgtctc cgtcgccgaa ctggacaagg cacgccgcac cgcgctcgcc
    16441 gacttcgaag cgcgcaaggc acaaccggac gccctcgcga atgaatggct cgatgcgatc
    16501 ttttataagt ccacgctcgc cgaagaggaa ctcgccctga acgccctgac gcctgccgac
    16561 gtgcaacgcg tggccgcgcg gcttttcgcg gaggcgaaac tggcaagcgt cgtcgtcggc
    16621 gatgcggcgc agttgcgcaa tgaactggcg cgtctgagcg gcggcatcga agaggccgga
    16681 acaaagcccg cgacgccgcc cgcttccgcc gccgagccga aaacgccggg gcgtccgtag
    16741 tcaattcaca aaaaaatcgc aagctgcctg caaccaatcg ctctgacgaa atcatttaag
    16801 gtgtcaacgc caacaagtct taatgaaact gaacgcaggc aggagcacgc gcgccgggtc
    16861 aactcgatgt tcgcgggcat tgccggtcgt tacgatcttt taaatcatct gttgtcggcg
    16921 aacatcgacc gtcgctggcg gcggctggtg gcggacgaac tgcgccccgc cctcgtcgcc
    16981 ggagcgcaga cgctcgacgt tgcctgcggc acgggcgacc tcgcgttgac cctcgccgaa
    17041 gccgggcagg cgcgcgtcac gggactcgat ttctgccgtc cgatgctcga agtggcgtcg
    17101 aagaaggctg cgcagcgaca acgcgggatc ccttttatcg aggccgacgc gctgcgcctg
    17161 ccctttgcgg cggagacgtt cgatgtcgtg accatcgctt tcggcctgcg gaatctggcg
    17221 agcgtcgaag gcggtctgcg cgagttgttt cgcgtgctca ggccgggcgg
//
LOCUS       DASWNS010000363         4316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_223245, whole genome shotgun sequence.
ACCESSION   DASWNS010000363 DASWNS010000000
VERSION     DASWNS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223245"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VGB76_12770"
     CDS             complement(<1..437)
                     /locus_tag="VGB76_12770"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY0099812.1"
                     /translation="MQPDPHPTSEQPLRGRTIITTRALAQSADFIAELERYGARVVPC
                     PTIEIVALESYELLDDALDHLYGYDWLIFTSTNGVDFFMRRMKERGLDVSELDELRVC
                     ATGAATADRLRDARVHVDVVPGEFKAEGVFEALADYLGGREAF"
     gene            complement(479..1417)
                     /gene="hemC"
                     /locus_tag="VGB76_12775"
     CDS             complement(479..1417)
                     /gene="hemC"
                     /locus_tag="VGB76_12775"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY0099813.1"
                     /translation="MREHFIIGSRGSKLALWQSEWVRSRLRELRPEAEIRIEVIKTSG
                     DVMLNVPLAVIGGKGVFTKELEEALLAGEIDLAVHSLKDLPTTLPDDLAITSITERED
                     ARDALVLREGVAARHNSLGELPRGAIVGTSSLRRQAQLKHLRPDVQIRDLRGNVDTRL
                     RKLDEGGYDAIILASAGLRRLGFERRIDAAISHEEMLPAVGQGALGIETRAGDARTAA
                     LVSLLDDAPTRAACTAERALLYALGGGCQVPIAAHATVRPGSLRLEALVAALDGGQVI
                     RDVIEDESAHAARAGATLAARLRERGAETLLAGLTL"
     gene            complement(1512..2780)
                     /gene="hemA"
                     /locus_tag="VGB76_12780"
     CDS             complement(1512..2780)
                     /gene="hemA"
                     /locus_tag="VGB76_12780"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY0099814.1"
                     /translation="MSIILVGLNHKTAPVEVRERLAFTDEACAETLQTLVDGELISEG
                     LIVSTCNRVEVLAATRGLNGAEGAARISTFLSETRSLPDNFFGQHLYTHTDEEAVRHV
                     FRVASSLDSMVVGEPQVLGQVRRAYSNAVTAGTAGRVLHRLVHHALHVAKRVRSETGI
                     ASSAVSISFTAVELGRKIFGSLKGTTVLVIGAGEMAELAAQHLAGAGASRILVTNRTT
                     ETAEQLAQKIKGRALDFSSLPAHLAEADIIICSTAAAGYVVTAEMAREALAARRQRPA
                     FFIDISVPRNVDPAVGELDNLFVFDVDDLEAVVASNIREREREAERAELIVESEVMQF
                     QQALRSLDLGPTVAALRQKLEEIARNELARQRGRLGELTPEQERAVEALLISTVNKIS
                     HPIINRLRRSHDTGEEENLKAWRNTFGLEE"
     gene            complement(2914..3798)
                     /gene="ccsA"
                     /locus_tag="VGB76_12785"
     CDS             complement(2914..3798)
                     /gene="ccsA"
                     /locus_tag="VGB76_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY0099815.1"
                     /translation="MPRFSFINYNAASKVFNCRLAPQMNYLLLTALVAYMNAAVHAVL
                     AFAYKRRSVERVAFVSLALGFAAHTAAFLRDWIQDGHYPLFGLRETLSFLAWTLVVAY
                     ALAHFRYQLKALGAFLMPLVAFLVTAAMLVRNSSDARAGTIAAGAAGWVFPIHTTFLI
                     FSYAAFFVVFAASAMYLWQERELKLKTFSALFHRLPSLTTVDDIGSTAAGIGFTLLSL
                     GIITGAILSSQQKGSIWNNDPKEVFALITWLLYLTMIHYRIQWRGRKSAIVGVAGFGL
                     VLFTFLGTRLMGGYHVFG"
     gene            3907..>4316
                     /locus_tag="VGB76_12790"
     CDS             3907..>4316
                     /locus_tag="VGB76_12790"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0099816.1"
                     /translation="MPERADAWELLCEYTKGESLRRHALAVETAMRACAARYGEAGAD
                     AEEWGMVGLLHDFDYEMFPDPEGGHPFKGANILCGRGYPDHFIRAIMGHATYTGVPRD
                     TPLARALFATDELCGFLVACALVRPSRSLDDMEVS"
BASE COUNT          845 a         1300 c         1399 g          772 t
ORIGIN      
        1 tcgaacgctt cgcgcccgcc cagataatca gctaacgctt cgaagacgcc ttcggctttg
       61 aactcgccgg gcacgacatc gacatgcacg cgcgcgtcgc gcagacggtc ggcggtggcc
      121 gcgcccgtcg cgcagacgcg cagttcgtcc aactcgctca cgtccagccc gcgctccttc
      181 atgcggcgca tgaagaaatc gacgccgttc gtgctcgtga agatgagcca gtcgtagccg
      241 tagagatgat cgagcgcatc gtcgagaagt tcgtaactct ctaaagcgac gatctcgatg
      301 gtaggacagg gaacgacgcg cgcgccgtag cgttcaagtt cggcgatgaa gtctgccgac
      361 tgtgcgagcg cgcgcgtggt gataatggtg cgaccgcgca ggggttgctc tgaggtggga
      421 tgtggatcgg gttgcatcac gggcgagcag gcgcaaactt cgaacgatca atctttattc
      481 aaagggtcag acctgcgagc aaagtttcgg ctccgcgttc gcgcaggcgt gccgcgagcg
      541 tcgcgcccgc gcgtgcggcg tgcgcgcttt cgtcttcaat cacgtcgcgg ataacttgcc
      601 cgccgtcgag cgcggcgacc agtgcttcga ggcgtagcga gccggggcgc accgtcgcgt
      661 gcgctgcgat aggaacctgg cagccgccgc caagcgcgta gagcagagcg cgttcggcgg
      721 tgcaggcggc gcgcgtgggg gcgtcgtcga ggagcgagac gagcgcggcg gtgcgtgcgt
      781 cgcccgcgcg cgtttcgatg ccgagcgcgc cctgcccgac ggcgggcagc atctcctcgt
      841 gagagatggc ggcatcaatg cgtcgctcga agccgagacg gcggagtccg gcggaggcga
      901 ggatgatcgc gtcgtagccg ccttcgtcca gcttgcgcag gcgcgtgtcc acgttgccgc
      961 gcaaatctct gatctgaaca tccgggcgta ggtgcttcaa ctgagcctgc cgtcgcaaac
     1021 tcgacgtgcc gacgattgcg ccgcgcggga gttcgccgag cgagttgtgt ctggccgcga
     1081 cgccttcgcg caagacgagc gcatcacgcg catcttcgcg ctcggtgatc gacgtgatgg
     1141 cgagatcgtc gggcaaggtc gtcggtaaat ctttcagcga gtggacggcc agatcaatct
     1201 ctccggccag cagagcctct tccagttctt tcgtgaagac gcctttgccg ccgataacgg
     1261 cgagcggcac gttcagcatc acgtcgccgg aagtcttgat cacctcgatg cgtatctccg
     1321 cttcaggtcg caactctctg agccgcgacc tgacccattc cgattgccac agcgcgagct
     1381 tcgagccgcg cgagccgatg atgaaatgct ctctcataaa aacagagggc ggaggtcagc
     1441 gttcagagtt tagtaggaag cagttcttct tttaactgat ctctaagctc tcagttctga
     1501 cccacgcctt tttattcttc cagcccgaaa gtgttgcgcc aggctttgag gttctcttcc
     1561 tcacctgtgt cgtgcgagcg gcgcaggcgg ttgatgatgg gatgcgaaat cttgttgacg
     1621 gtggagatca gcagggcttc gacggcgcgt tcctgttccg gcgtcaactc gccgagccga
     1681 cctcgctggc gcgccagttc gttgcgcgcg atctcctcca acttctgccg gagcgcggcg
     1741 accgtgggac ctagatcgag cgagcgcagg gcttgctgga actgcatcac ttcggattcg
     1801 acgatcaact ccgcgcgttc ggcctcgcgt tcgcgttcgc gtatgttgga ggcgacgacc
     1861 gcttccagat cgtctacgtc gaagacgaac aggttgtcga gttcgccgac agccggatcg
     1921 acgttgcgcg gcacgctgat gtctatgaag aaggccggac gctgccgccg cgccgccagt
     1981 gcctcacgcg ccatctccgc cgtgacgacg tatccggcgg cggcggtcga gcagatgatg
     2041 atgtcggctt cggccagatg cgcgggcagg gaagagaagt cgagcgcgcg gcctttgatc
     2101 ttttgcgcga gttgctcggc ggtttccgtc gtgcgattgg tgacgaggat acgagacgcg
     2161 cccgcgcccg ccagatgttg cgcggcgagt tcggccatct cgcccgcgcc gatgacgagc
     2221 acggtcgtcc ccttgagcga gccgaaaatt ttgcgcccca attcgaccgc cgtgaaactg
     2281 atcgagacgg cggaggaagc gatcccagtc tccgagcgca cgcgcttggc gacgtgcagc
     2341 gcgtgatgca cgaggcgatg gaggacgcgc cccgccgtgc ccgccgtgac cgcgttcgag
     2401 taggcgcgtc gcacctgacc gagcacctgc ggctcgccga cgaccatcga atcgagtgaa
     2461 gaggcaacgc gaaaaacgtg tcggacggcc tcctcgtccg tgtgcgtgta gaggtgctgt
     2521 ccgaagaaat tgtcgggcag ggagcgcgtc tcgctcagga atgtgctgat gcgcgccgcg
     2581 ccctccgcgc cgttcaaacc gcgcgtcgcc gccagcactt ccacgcggtt acaggtggaa
     2641 acgatcaagc cttcgctaat caactcgccg tcaaccaggg tctgcaaagt ctcggcgcac
     2701 gcctcgtccg taaatgccag acgctcacgc acctccaccg gggcagtctt atgattgagt
     2761 ccgacgagaa tgatagacat aactaaaagg atgaacgcgg aacgaagaac taaacacagg
     2821 agtcagaatt cagaagtcag aatttcggaa gtcggaataa acccggcgcg ggtcttcctg
     2881 ctgaattctg ttttctcctt catcatttat cgttcatccg aagacgtgat agccgcccat
     2941 gaggcgcgtg ccgagaaagg tgaagaggac gaggccgaaa ccggcgacgc cgacgatagc
     3001 ggacttgcgc ccgcgccatt ggatgcgata gtgaatcatg gtcaggtaga gcagccatgt
     3061 gatgagcgcg aagacttcct tcgggtcgtt gttccaaatg ctccctttct gttgtgaaga
     3121 gaggatcgcg cccgtgatga tgccgagcga gagcagcgtg aagccgatcc cggcggcggt
     3181 cgagccgatg tcgtcaacgg tcgtcagcga cgggagacgg tgaaagagcg cggagaaggt
     3241 tttcagtttc aactcgcgct cttgccagag atacatcgcg ctcgcggcga agacgacgaa
     3301 gaaggcggcg taggaaaaga tgagaaacgt cgtgtggatg ggaaagaccc acccggctgc
     3361 gcccgccgcg attgtccccg cgcgcgcgtc ggaagagttt ctcacgagca tcgccgccgt
     3421 gacgagaaat gcgacgagcg gcatcaggaa cgcgccgagc gctttgagtt gatagcgaaa
     3481 gtgagccagc gcgtaagcga ccacgagcgt ccaggcgaga aaggatagag tctcgcgcag
     3541 gccgaagagc ggatagtggc cgtcctgtat ccagtcgcgc aggaaagcag ccgtgtgcgc
     3601 cgcaaagccg agcgcgagcg acacgaaggc cacgcgctcc acggagcgtc gcttgtaagc
     3661 gaacgcgagg acggcgtgaa ccgccgcgtt catgtaggcg accagagctg tgagcaggag
     3721 ataattcatc tgaggcgcga gacgacaatt gaaaaccttg ctggcggcat tgtagttgat
     3781 gaatgaaaaa cgcggcaagc gggcaatccc gaaacgcgct gttgtcacca aacgccaaga
     3841 cgtgagaaac ttcccggcgt gccaacctcc cgttgtcgtc taaatttgag tcgaaggtaa
     3901 acgaccatgc ccgaacgcgc cgacgcctgg gaactcctgt gcgaatacac gaaaggcgag
     3961 agcctgcgcc gccacgcgct cgccgtcgag acggccatgc gcgcctgcgc cgcgcgctac
     4021 ggcgaagcgg gcgcagacgc agaggaatgg ggcatggtcg gcctgctcca cgatttcgac
     4081 tacgaaatgt ttcccgaccc ggaaggcggg catcccttca aaggcgcgaa catcctgtgc
     4141 gggcgcggct acccggacca cttcatccgt gccatcatgg gacacgccac ttacacggga
     4201 gtgccgcgcg atacgccgct cgcacgcgcc ctgtttgcga ccgacgaact ctgcggcttt
     4261 ctcgtcgcct gcgcgctcgt gcgcccttcg cggagtctgg acgacatgga agtctc
//
LOCUS       DASWNS010000364         6875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_196728, whole genome shotgun sequence.
ACCESSION   DASWNS010000364 DASWNS010000000
VERSION     DASWNS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196728"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VGB76_12795"
     CDS             <1..580
                     /locus_tag="VGB76_12795"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0099817.1"
                     /translation="PSPRLQIEGVYEMRTLTIAKPEETEYLPYYGRYVSLVQEGDILA
                     TLDRQAAETLGLLRAIPESQAGFRYAPGKWSIKELVGHIIDTERIFAYRALRFARNDR
                     TPVPGYEQDDYIREASFDDCLLAALAAEFESVRHASLFLFKHLHEAAWTRRGVANESE
                     VSVRALAYIIAGHELHHREILRHRYLQTGQAN"
     gene            857..1489
                     /locus_tag="VGB76_12800"
     CDS             857..1489
                     /locus_tag="VGB76_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099818.1"
                     /translation="MKYSFRFYPALAVMLIAASVQGLAQTGKDAQGAQEQAASPFPRI
                     AYDERAWKPFESTEGGFTIAFPGKPGFQIQTVEAAIGNLFNKTYGLDIGVCFFAVSFA
                     DYPAPVPLHDKELINRSLDAGRDRALAGTGSKLISEAPLTLDGYIGRYVLSADGEGMT
                     HNQSYVVGNRFYQVSVRTDDYRNSPAADQKFFKDLVNRFFTSFKLTRKAY"
     gene            1529..2242
                     /locus_tag="VGB76_12805"
     CDS             1529..2242
                     /locus_tag="VGB76_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099819.1"
                     /translation="MKRYLRALSTSFALATFMAAVATAQTAGPAARPEQPPRPPKSIT
                     PKEPVGAQPDLGTIQNSVYTNDYFRLRIPVPAGWNIHDEASKRRLMQSGNEELSASKP
                     EMAGKLDKSLQRTLNLLALDNLTIPSTGGGSAALLIGAEPIPRGQTLTVAQYMETTKR
                     LVMSVPGYEMVEEIHTETIGGAECSAMTLKRNLAGVTMKQKYIGIIRKGYALFFVTAY
                     TNEDAGRTLDDILKGSRLN"
     gene            complement(2349..3041)
                     /locus_tag="VGB76_12810"
     CDS             complement(2349..3041)
                     /locus_tag="VGB76_12810"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEY0099820.1"
                     /translation="MRQQYLDFLNREPDENGFNAWVRVLAGCAYEGHFGPGKSGSDPA
                     CDRITVSSSFFRSTEFQFKGFFVYRFYKASLGRVPTYEEFLRDMTSVTGQTEAEVVAR
                     REAFADAWVERADFVALNEGITNAEFVEGLAAKAGVTIAGRAQLVSDLNDGRRTRAQA
                     LRAVVESPELFNREYNAAFVLMQYFGYLQRDPDEAGYNNWLSLLNRTGDHRTMIFGFL
                     YSQEYLLRFGQP"
     gene            complement(3014..>6875)
                     /locus_tag="VGB76_12815"
     CDS             complement(3014..>6875)
                     /locus_tag="VGB76_12815"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0099821.1"
                     /translation="EIRIYKNGRLDGYGTGGVGKTLKYQTNYTAKIGEWGEGHNGNPV
                     YRFGGAIDEPAIFNRALSQTEIQNVYDARNRGMCLASAAQNITASNTNDSGAGSLRQA
                     ILDANASPGTQTINFQIPGAGVHTINLTSPLPDITDPVVIDGYTQPGARVNTLAAGSN
                     AVLLIELVGPGAGGSGLQLRGGNSTVRGLVSNRFDNSAAININTGNNNRVEGCFLGTD
                     ANGTAKFADESREGVSINASTGNVIGGTTPATRNVISGHRLNGINIFGAGAGNNLVQG
                     NLIGTDKTGAAALGNGQVGVSISGFNGNVIGGTTAAARNVISGNHTGLQIFTGANAVQ
                     GNYIGTDASGTVDLGNSGVGLFMQGGASNLIGGLTPTPGQAPGNIISGNGSAGIDING
                     NESPGHTIQGNLIGVDVGGASALPNNGDGISIRNSGGGNLVGDANAAGRNVVSANAGH
                     GISLNNNNNSLRNNYVGVGSDGAAALGNTQNGVLINSRDDNALASNRIAFNGQDGIAV
                     NFFSLAGVNNSFLSNSIFSNTGLGIDLGNNGLTANDAGDADAGANNLQNFPVVSSALF
                     SNGAATASGTLNSTANTTFTLEFFSNAACDNNGEGQTLAGTTSVTTDAGGNATFSLVL
                     SNALVAGQFVTATATDPTGNTSEFGACAQVSAAPGAFTISGQILEGADGLSGVTVTLS
                     GTQTATTTTDAGGNYSFVNLPAGGNYTVTPARPNYSFTPPRRAFNNLSANQTANFTGT
                     PAQSQPVSGRVSDGSGGGIANVTLYVTDATTGANIITTLTDANGNYALMLPPGGDYTI
                     TTAKAGLTFQPAARTFANLSGGQTSDFTAARGVTISGRVTGSGGNGIGNVNVTLSGSV
                     TRTTLTLPNGSFLFNNLHANGSYVVRADSGLLTFTPPQIIRPALASDATFNLVAAPRP
                     LPVPMPPIEDDFDGTTRDPSKFTEGTLTQPPGSKDPQVTVVQEGGKLKITPRGGISET
                     SFNGYVTVRAIDFTDAQASVEVNQTADNGAQTIFAAGRDERNFFRFIAQDVDAPPGAA
                     SSKAGAVARTNGLRRLIFQARSTGVLNGLSPGIPFDPLQHRYWRFRHSSAESAIFFET
                     SSDRVVWTEQRRVALGGAIGAFAVELSAGTSGLVMNPGQAIFDNLLVQPSTVVNRANN
                     IRLAQSSYRVSEGAGVLTLKAVRAGDTSRETRLDYATEPHDGKPCNTQDGKARARCDF
                     GTTAGTIRFAPAKRRRVSASSSQTTSTRKATRRFALRSASLQPESSKNPLRLSSRLPT
                     TTRARRRIRSIPPRSSCASSISTS"
BASE COUNT         1199 a         2071 c         2283 g         1322 t
ORIGIN      
        1 gccctcgccg cgcctgcaaa tagaaggagt ttatgagatg aggactttaa ccatcgccaa
       61 gccggaggag acggaatacc tgccctacta cggcaggtac gtctcgctcg tacaggaggg
      121 agatatactg gcgacgctcg acaggcaggc agccgagacg ctcggcctcc tgcgcgccat
      181 cccggaatcg caggcgggct ttcgttacgc gcccggcaag tggagcatca aggaactcgt
      241 cgggcatatc atcgacaccg agcgcatctt cgcctatcgc gcgctccgct tcgcgcgcaa
      301 cgaccgcacg cccgtacccg gctatgagca ggacgactac attcgcgagg cttccttcga
      361 tgattgcctg ctcgccgcgc tggctgcgga gtttgagagc gtcaggcatg ccagtctctt
      421 cctcttcaag catctgcacg aagcggcctg gacccgccgg ggcgtggcta acgagagcga
      481 agtgagcgtc cgcgcgctgg cctatattat cgccgggcac gaactgcacc acagggagat
      541 tctccgccac aggtacctgc aaaccgggca agccaactga tgcgccgtcg cctcagacgc
      601 agcggtgttg tttgagacaa ctgcctgcgg ggcgttcaac gtcagagcac atgcggcagg
      661 cagccgcgag cgggtctggc gtccacgacg gagcgtgaag gctcgattgt ttgttattat
      721 ttccctgcgc cggacgaagg cgggacgcaa cgccatctct gctcacgcgc ggctgtcggg
      781 agcacgacgc gtgcgcgacc gtgacacatg accggagagc acgaattact ttttgacgga
      841 gagcgagtga agcgacgtga aatatagttt cagattctat ccggcgctgg ccgtgatgtt
      901 gatcgcggct tccgttcaag ggctggcgca gacgggcaag gacgcgcagg gcgcgcagga
      961 gcaggcggct tcgcccttcc cgcgcatcgc gtatgacgag cgggcttgga agccgttcga
     1021 atcaactgaa ggcggcttca ccatcgcctt ccccggcaag cccggttttc agatacagac
     1081 cgtagaggcc gcaatcggca acctcttcaa taaaacttac ggactcgaca tcggcgtctg
     1141 tttcttcgcc gtctcgttcg cagactaccc ggcacccgtc cctctgcacg acaaagagtt
     1201 gatcaaccgc tcactggacg cgggacgcga ccgcgcgttg gcgggtacgg gcagcaagct
     1261 catcagcgaa gcgccgctta cgctcgacgg ctatatcggg cgctacgttt tatccgccga
     1321 cggcgagggc atgacgcaca atcaaagcta cgtcgtcggc aatcgcttct accaagtctc
     1381 cgtccgcacg gatgattatc gcaacagccc tgcggcggat cagaagtttt tcaaagacct
     1441 cgtcaaccgt tttttcactt cgttcaagtt aacgaggaaa gcctactagc ccgtaagttc
     1501 tcaaacctcc gagacaggaa tccaccagat gaaacgctac ctccgcgctc tctcaacttc
     1561 tttcgcgctg gcgactttca tggccgccgt cgcgacggct caaacggctg gtcccgcagc
     1621 aagaccggag cagccgcccc ggccacccaa aagcatcacg cccaaagagc ctgtcggcgc
     1681 gcagcccgac ctcggcacga tccagaactc cgtctatacg aatgattact ttcgcctacg
     1741 catcccggtt cccgccggtt ggaacatcca cgacgaagcg agcaagcggc gtctgatgca
     1801 gagcgggaac gaagaactgt cggcctccaa gcctgagatg gcgggcaagc tggacaagtc
     1861 tttgcagcgg acgctgaatc tgctggcact cgacaacctc accatcccgt caaccggcgg
     1921 cggatcagcc gcgctgctca tcggagccga gcctattccg cgcgggcaga cgttgaccgt
     1981 ggcgcagtac atggaaacaa cgaagcgtct ggtgatgagc gtgccggggt atgagatggt
     2041 ggaggagatt cacaccgaga cgataggcgg cgcggagtgc agcgcgatga cgctcaagcg
     2101 caacctggcg ggtgtgacga tgaagcagaa gtacatcggc atcatcagga agggctacgc
     2161 gctctttttc gtcacggcct acacgaacga agacgcaggc cgcaccctcg acgacatcct
     2221 caaggggagc aggttgaatt agaagtaaga cgcagagaag aaacgatgaa gtaaaaacga
     2281 tgaaggatga acggaagccg atgcttcttc tgttcatcct tcatcgttct tacttcatcg
     2341 tttcctgttt acggctggcc gaatctcaac agatattcct gcgagtagag gaagccgaag
     2401 atcatcgtgc ggtggtcgcc cgtgcggttg agcaggctca accagttgtt gtaacccgct
     2461 tcgtcagggt cgcgctgcaa atagccgaag tactgcatca acacgaaggc cgcgttgtat
     2521 tcgcggttga acagttccgg actctccacc acggcgcgca acgcctgtgc gcgcgtcctg
     2581 cgcccgtcgt tcagatcgga gactaactgc gcgcgcccgg cgattgtgac gcccgccttc
     2641 gccgccagcc cctctacgaa ctcggcgttt gtgatgcctt cgttgagcgc gacgaaatcc
     2701 gcgcgctcga cccacgcatc ggcgaaggct tcgcggcgcg cgaccacttc cgcttccgtc
     2761 tgcccggtca cgctcgtcat gtcgcgcaaa aactcttcat aggtcggcac gcgcccgagg
     2821 ctcgccttgt agaagcgata gacgaagaag cctttgaact ggaactccgt cgaacggaag
     2881 aacgaagaag agacggtgat gcggtcgcac gccggatcgg agcctgactt gccggggccg
     2941 aagtggcctt cgtaggcgca ccccgcgagc accctgaccc atgcgttgaa accgttttcg
     3001 tccggctcgc ggttcaggaa gtcgagatac tgctggcgca cgaggaacgg ggcggtattg
     3061 atcggatgcg gcgtcgtgcc cgtgtcgttg tcggcaatcg tgacgagagc cgaagcgggt
     3121 tcttcgacga ctccggctga agggaagccg agcgcaacgc gaatcgtctc gtcgccttcc
     3181 gcgtagatgt cgtctgtgat gaagacgctg aaactcttct ccgtttcgcc ggggcgaagc
     3241 gaatcgtgcc cgccgtcgtg ccgaagtcgc agcgcgcacg cgccttgccg tcctgcgtgt
     3301 tgcagggctt gccgtcgtgc ggctcggtcg cgtagtcgag gcgcgtctcg cgcgacgtgt
     3361 cgcccgcccg tacggccttg agcgtcaaca cgcctgcccc ttcgctcacg cgataagagc
     3421 tttgggcgag ccggatgttg ttcgcgcggt tgacgacggt gctcggctgc acgagcaggt
     3481 tatcgaagat ggcctgtccg ggattcatga ccagaccgga agtgcccgcg ctgagttcga
     3541 cggcgaacgc gccgattgcc ccgccaagcg cgacgcgccg ttgctccgtc cagaccacgc
     3601 ggtcagaact cgtctcgaag aagatcgcgc tctcagccga actgtgccgg aaacgccagt
     3661 agcgatgctg caaaggatcg aaaggaatac ccggcgagag gccgtttaag acgcccgtgc
     3721 tgcgcgcctg aaagatcagg cggcggagtc cgttagttcg cgcgacggca cccgctttgg
     3781 acgacgcggc gccgggcggc gcgtcaacat cctgcgcgat gaagcggaag aaattgcgct
     3841 cgtcgcgccc ggcggcgaag atcgtctgcg cgccgttgtc ggcggtttga ttgacttcga
     3901 cgctcgcctg cgcgtctgtg aagtctatcg cgcgcacggt gacgtagccg ttgaaactcg
     3961 tttcgctgat gccgccgcgc ggcgtgatct tcaacttccc gccttcctgc acgaccgtca
     4021 cctgcgggtc ttttgaacca ggcggctgcg tgagcgtgcc ctctgtgaac ttcgaggggt
     4081 cgcgcgtcgt gccgtcgaag tcgtcctcga tgggcggcat cgggacgggc aaggggcgcg
     4141 gcgcggcgac gaggttgaac gtcgcatcgg aggcgagcgc ggggcggatg atctgcggcg
     4201 gggtgaaggt gagcaggccg ctatccgccc gcacgacgta gctgccgttg gcgtgcagat
     4261 tgttgaagag gaaacttccg ttcggcagcg tcagcgtggt gcgcgtgacg gaaccgctca
     4321 gagtgacgtt gacgttgccg atgccgttgc cgcccgaacc tgtgacgcgc ccgctgatcg
     4381 tcacacctct ggccgcagtg aagtcggaag tctggccgcc gctcaggttt gcgaaggtgc
     4441 gcgcggcggg ttggaaagta agcccggctt tggcggtcgt gatcgtgtag tcgccgcccg
     4501 gcgggagcat caaggcgtaa ttgccgttgg cgtccgtcaa cgtcgtgatg atgttcgcgc
     4561 ccgtcgtcgc gtccgtgacg tacagtgtga cgttggcgat gccgcctccg ctgccgtccg
     4621 atacccgacc gctgaccggc tgcgactgcg cgggcgtgcc cgtgaagttg gccgtctggt
     4681 tcgcgctcag gttattgaag gcgcggcgcg gcggcgtgaa actgtagttc gggcgcgcgg
     4741 gcgtcacagt ataattgccg cccgccggaa gattcacgaa cgaatagttg ccgcccgcat
     4801 cggttgtcgt cgtggcggtc tgcgttccgc tcagggtgac ggtgacgccg gagaggccgt
     4861 ctgcgccttc gaggatttga ccgctgattg tgaacgcgcc cggcgcggcg gacacctgcg
     4921 cgcacgcgcc gaactcggac gtgttgcccg tggggtcggt cgccgtggcc gtgacgaatt
     4981 gaccggcgac gagcgcgttc gagagcacga ggctgaaggt ggcgttgccg cccgcgtccg
     5041 tcgtgacgga agttgtcccg gcgagcgtct gcccttcgcc gttgttgtcg caggcggcgt
     5101 tggagaagaa ctccaacgtg aaagtggtgt tggccgtcga gttgagcgtg cccgacgcgg
     5161 tcgccgcgcc gttcgagaag agggcggaag agacgacggg gaaattttgc agattgtttg
     5221 cgcccgcgtc cgcgtcgccc gcgtcgttgg ccgtcaggcc gttattgccg aggtcgatgc
     5281 cgagtcccgt gttcgagaag atggagttgg acaggaacga gttgttcacg cccgccaagg
     5341 agaagaagtt gacggcgatg ccgtcctgac cgttgaaggc gatgcggttg gacgcgagcg
     5401 cgttatcgtc gcgcgagttg atgaggacgc cgttttgcgt gttgccgaga gccgccgcgc
     5461 cgtccgaccc gacgccgacg tagttgttgc gcaggctgtt gttgttgttg ttcagagaga
     5521 ttccgtgtcc ggcgttggcg gagacgacgt tgcgccctgc ggcgtttgcg tcgccgacga
     5581 ggttgccgcc gccgctgttg cggatagaga tgccgtcgcc gttattgggg agggcgctcg
     5641 cgccgcccac gtccacgccg atgaggttgc cctgaatggt gtggccgggc gattcgttgc
     5701 cgttgatgtc gatgcctgcg ctgccgttgc cggaaatgat gttgcccggt gcctgtcccg
     5761 gcgtgggcgt caggccgcca atgaggttgg acgccccgcc ctgcatgaag aggccgacgc
     5821 cgctgttgcc gagatcaact gtgccggacg cgtccgtgcc gatgtagttg ccctgcacgg
     5881 cgttcgcgcc ggtgaaaatc tggaggccgg tgtggttgcc ggaaatgacg ttgcgcgccg
     5941 ccgcagtcgt gccgccgatg acgttgccgt tgaagccgct gatgctgacg ccgacctgcc
     6001 cgttgccgag ggcggctgcg ccagtcttgt ccgtgccgat caaattgccc tgcacgagat
     6061 tgttgcctgc cccggcgccg aagatgttga tgccgttcag gcgatggccg gagatgacgt
     6121 tgcgcgtcgc gggcgtggtg ccgccgatga cgttgccggt gctggcgttg atgctcacgc
     6181 cctcgcggct ctcgtcggcg aatttggccg tgccgttcgc gtccgtaccg aggaaacaac
     6241 cttcgacgcg gttgttgttg ccggtgttga tgttgatggc agccgagttg tcgaagcggt
     6301 tgctgacgag gccgcgcacg gtcgagttgc cgccgcgcag ttggaggccg gaaccgcctg
     6361 cgccggggcc gacgagttcg atgagcagga cggcgttcga ccccgccgcg agcgtgttca
     6421 ccctcgcgcc cggctgcgtg tagccgtcaa tgacgacggg gtcggtgatg tcgggcagcg
     6481 gcgaagtcag gttgatcgtg tggacgcctg cgccggggat ttggaagttg atggtctgcg
     6541 tgccggggct ggcgttggcg tcgaggatgg cctggcggag cgaacccgcg ccggagtcgt
     6601 tcgtgttcga ggcggtgatg ttttgcgccg cagaggcgag acacatccca cggtttctgg
     6661 cgtcgtagac gttctgaatt tcggtctgcg agagggcgcg gttgaagatg gccggttcgt
     6721 caatcgcgcc gccgaatctg tagacggggt tgccgttgtg tccctcgccc cactcgccga
     6781 tcttggccgt gtagttcgtc tggtacttga gcgtcttgcc cacgccgccc gtgccgtagc
     6841 cgtcgaggcg tccgttctta taaatcctga tctcg
//
LOCUS       DASWNS010000365         4230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_196843, whole genome shotgun sequence.
ACCESSION   DASWNS010000365 DASWNS010000000
VERSION     DASWNS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196843"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VGB76_12820"
     CDS             complement(<1..726)
                     /locus_tag="VGB76_12820"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY0099822.1"
                     /translation="MNTIRTIGLVTGGGDAPGLNAVIRAVVKTAVTEYNLRVVGIEDS
                     FEGLIGETSVRELKPADVRGLLPRGGTILGTRNRGRFVERGGVDGEDIKPEEVYLEAT
                     DNLQRLGIDALVVLGGEGTLGIAAEFDELGVPVIGVPKTIDNDLAFTELTFGFVTALD
                     IATEALDRLHTTAASHDRVMILEVMGRHTGWIALHSGIAGGADVILIPEIPFSMQSVA
                     EKVYERERCDSQFSIIVVSEGARE"
     gene            complement(892..1173)
                     /locus_tag="VGB76_12825"
     CDS             complement(892..1173)
                     /locus_tag="VGB76_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099823.1"
                     /translation="MAVQREIEMRGIPTVLITVSPEVSAQMRPPRALYPKGFKIGNSL
                     GRPGMRTLQRQVLMDALSLLSENTRPGQIVTREYPDYGEQYDPPSVKAK"
     gene            complement(1198..1626)
                     /locus_tag="VGB76_12830"
     CDS             complement(1198..1626)
                     /locus_tag="VGB76_12830"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HEY0099824.1"
                     /translation="MTTLARKCVPFTPFERELAKSNIAIVTAGGVHLREQEPFNIADE
                     LGDLGFRIIPPDTDSAQLRVTHHHYDHTDADADINVVFPLDVLRELEADKVIGGLARK
                     HVGYMGYTMQLKAMYEGTAPEIANEIDKGSRADAVILTGG"
     gene            complement(1721..2185)
                     /locus_tag="VGB76_12835"
     CDS             complement(1721..2185)
                     /locus_tag="VGB76_12835"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2045..2047),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0099825.1"
                     /translation="MPEVGDIAPDFELGSSLEKGKKVRLSDFRGNKNVVLAFYPLAWT
                     PVUTAQMPAYEADLERFAGYDAQVLGISVDSVPCNTAWAKSLGGLSYDLLSDFEPKGE
                     VARSFGAYRDKDGISERAIFIIDKEGKIAYKDIHDISDQPDNEDLFEVLRNL"
     gene            2422..3594
                     /locus_tag="VGB76_12840"
     CDS             2422..3594
                     /locus_tag="VGB76_12840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY0099826.1"
                     /translation="MKPATINISRKGAERARAGHLWIYRSDLLDGGEATPGGAIVQVR
                     DERGRFVGSALYSDRSQIALRLLTWREETIDRDWWRERLRATIARRAGLAGEADAFRL
                     VYAEGDALPSLIVDRYGDVLVLQTLSQATDALKEMLVELLVELLAPRAVIERNDVRVR
                     QLEGLPLTAGVLYGDAPEEMEVVQHGTRFRIAPLGGQKTGAFLDQRENHLAARRLAYG
                     RALDCFTFNGAFALHMAQTCESVLGLDISAEAVAASRRNAESNNLTNVEFREANVFDA
                     LREMQDAGERFDTIVLDPPAFAKNRASVRAAARGYKEINLRALKLLNAGGLLITCTCS
                     YHMNETQFHEVIAEASADARRRLQIVEKRIQASDHPVLVGVPETLYLKCIIARAVD"
     gene            3892..4191
                     /locus_tag="VGB76_12845"
     CDS             3892..4191
                     /locus_tag="VGB76_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099827.1"
                     /translation="MKANVRKLGSGEAARVELKLWDGRKLKGYIREAGEDTFVVAETK
                     TAVVTTVPYTEVRQIKGSNRLTAAKVGLTLAKGVAIVGAVALGAMLLVLISVPKS"
BASE COUNT          835 a         1294 c         1242 g          859 t
ORIGIN      
        1 ctcgcgcgcg ccttccgaga cgacgatgat gctgaactgc gaatcgcaac gctcgcgctc
       61 gtaaactttt tccgcgaccg attgcatcga aaaaggaatt tcgggaatga ggatcacgtc
      121 tgcgccgccc gcgatgccgg aatggagcgc gatccagccc gtgtgcctgc ccatcacttc
      181 gaggatcatc acgcggtcgt gcgaggcggc ggtcgtatgc aggcggtcta aggcttccgt
      241 cgcgatgtcg agcgcggtga cgaagccgaa cgtcaactcc gtgaaggcca gatcgttatc
      301 aatcgtcttc ggcacgccga tgaccggcac gcccaactcg tcgaactccg ccgcgatgcc
      361 gagcgtgcct tcgccgccga gcacgacgag cgcgtcgatg ccgagccttt ggaggttgtc
      421 cgtcgcctct agatagactt cttcgggctt gatgtcctca ccgtcaaccc cgccgcgttc
      481 gacgaaacgg cctcgattgc gcgtgccgag gatcgtgccg ccgcgcggca gcagtccgcg
      541 cacgtccgca ggtttcaact cgcgcacgct cgtctcgccg atcaaccctt cgaagctgtc
      601 ttcgatgccg accacgcgca ggttgtactc cgtcacagcg gtcttcacca ccgcgcgaat
      661 caccgcgttc agtcccggcg catcgccgcc gcccgtcacc agcccgattg ttcgtatcgt
      721 attcataagt gatgagtaat gagtgataag taatgagtga tgagtgacaa gcgaaaagcc
      781 gtaaatcgtg aatcgtcaat cgtgaataga agacgatctt tctctattta cgattcacga
      841 ttcacgatct acttgttttt actcatcact catcactcat cacccatcac tttattttgc
      901 cttgaccgag ggcgggtcgt actgctcgcc gtagtcgggg tattcgcgtg tgacgatctg
      961 gccggggcgc gtgttctcgg aaagcagcga gagtgcgtcc atgagcacct gtctctggag
     1021 agtgcgcatg cccggtcgtc ccagactgtt gccgatcttg aagcccttcg gatagagagc
     1081 gcgcggcggg cgcatctgcg cagagacttc cgggctgacc gtgattagga ctgtcgggat
     1141 gccgcgcatt tcgatttcgc gttgaaccgc cacgaccgtg cggtgacaga cgttcggtca
     1201 accgccggtt aagatgacgg catcggcgcg gctgcccttg tcgatctcgt tggcgatctc
     1261 cggcgcagtc ccttcgtaca tggccttgag ttgcatcgtg tagcccatgt agccgacgtg
     1321 cttgcgcgcc agcccgccga tcactttgtc ggcttcgagt tcgcggagca cgtcgagcgg
     1381 gaagacgacg ttgatgtcgg cgtcggcgtc cgtatgatcg tagtgatgat gcgtgacgcg
     1441 cagttgtgcg gagtcagtat cgggcgggat gatgcgaaag cccagatcgc cgagttcgtc
     1501 cgcgatgttg aaaggctcct gctcgcgcag gtggacgccg cccgccgtca cgatggcgat
     1561 gttgctcttc gccagttcgc gctcgaacgg cgtgaagggc acgcactttc ttgccagtgt
     1621 agtcataatt gtttaagagg ccggaggcgc gaggttagtg aaaacaagaa atgaagactc
     1681 tcttaactaa ctctaacctc tcacctccga cctctttccc ttacaggttg cgcagcactt
     1741 caaataaatc ttcgttgtcc ggctgatccg agatgtcgtg gatgtctttg taagcgatct
     1801 tgccttcttt atctatgatg aagatggcgc gttcggagat accgtctttg tcgcggtatg
     1861 cgccgaacga acgtgccact tcacccttcg gctcgaaatc gctcaacaag tcataagaga
     1921 gtccgccgag tgatttcgcc caggcggtgt tacacgggac gctgtcaact gaaatgccca
     1981 gaacctgagc atcatagcct gcgaagcgtt cgaggtctgc ttcataggcc ggcatctgcg
     2041 ccgttcagac gggcgtccag gccagcgggt aaaacgccag caccacattt ttgttgccgc
     2101 gaaaatccga gagtcgcacc ttcttgcctt tttcaaggct ggagccaagc tcgaagtccg
     2161 gggctatatc tcctacttca ggcatcttga gataaccctc cttccaagtg tttgcatgaa
     2221 aactgaattg ttgacctgtg attcaacata cgcgtttaga ctacatgcaa aagcctctcc
     2281 gttcaagcac cagcgccttc tgattttgtc acgcgaatat ggcgcagtag cgtcatgtca
     2341 gatcaggcga gcggcgggcg cgggcgtgct ttacaaacag acgcccggca gggcaaagtc
     2401 actacgaaaa ttctaaactc catgaaacca gcaaccatca acatctcacg caaaggcgcg
     2461 gagcgcgcac gcgccgggca tctctggatc tatcgcagcg acctgctcga cggcggcgag
     2521 gcgacgccgg gcggcgcaat cgttcaggtg cgcgacgaac gcggtcgctt cgtcggttcc
     2581 gcgctctaca gcgaccgctc gcagatcgct ctgcgcctcc tgacgtggcg cgaagagacg
     2641 atagaccgcg attggtggcg cgagcgcctg cgcgccacca tcgcgcgccg cgcgggactc
     2701 gcgggcgagg ccgacgcctt ccgtctcgtc tacgcagagg gcgacgcgct gccctcgctc
     2761 atcgtagacc gctacggcga tgtgctcgtc ctccagacgc tctcgcaggc gacggacgcg
     2821 ctcaaggaga tgctcgtcga actgttagtg gaactgctcg ctccccgcgc cgtcatcgaa
     2881 cgaaacgacg tgcgcgtgcg tcaactcgaa ggcttgccgc tcacggcggg cgtgctctac
     2941 ggcgacgcgc ccgaagagat ggaggtcgtg cagcacggca cgcgttttcg catcgcgccg
     3001 ctcggcgggc agaagaccgg agcgtttcta gaccagcgcg aaaaccacct cgccgcgcgt
     3061 cgcctcgcct acgggcgcgc gctcgattgc ttcaccttca acggcgcgtt cgcgctccac
     3121 atggcgcaga catgcgagag cgtcctcgga ctcgacatct ccgccgaagc tgtcgccgcc
     3181 tcccgccgca acgccgaatc gaacaactta accaacgtcg agttccgcga ggccaacgtc
     3241 ttcgacgccc tgcgcgagat gcaggacgcg ggcgaacgtt tcgataccat cgtgctcgac
     3301 ccgcccgcct tcgccaaaaa ccgcgcgagc gtccgcgccg ccgcacgcgg ctacaaagag
     3361 atcaacctgc gtgccctcaa actcctcaac gcaggcggcc tgctcatcac ctgcacctgc
     3421 tcttaccaca tgaacgagac gcaattccac gaagtgatcg ccgaagcctc cgccgacgcg
     3481 cgccgtcgct tgcagatcgt ggagaaacgc attcaggcca gcgaccaccc ggtgctcgtc
     3541 ggcgtccccg aaaccctcta cctcaaatgc atcatcgcgc gggcggtcga ttaaaatttc
     3601 agacgcgtgc gcctgcgctg gcgagcaacc gccggaaata tctttcaggt gtcagcacaa
     3661 atcaaaaagt aaaaaagccc tctcctgtgt ttcaagcggc tgttggcggc agatgaactg
     3721 cggcgcgggg attgctttgt gactcttcgg agattttcga ctccgaaggg aaaagttatg
     3781 ttcaagaaag ttctatcatt aacgctcgtc gctcttttga ttaatgtgtt cgggatgacg
     3841 ccggttcgag ccggtgcgcg ggtcgtaggg cagactcgta atgtcgagaa ggtgaaagcg
     3901 aatgttcgta agctcggatc gggcgaggcc gcacgcgtcg agttgaaact gtgggacggt
     3961 cggaagctga agggctacat ccgcgaagcg ggtgaggata cctttgtcgt cgcggagacg
     4021 aagaccgccg tggtgaccac cgtgccttac acagaggtca ggcagatcaa agggagcaac
     4081 cgtttgaccg ccgccaaagt cgggctgacg ctcgccaaag gcgtagcgat agtcggcgcg
     4141 gtggctctcg gcgcgatgct gctggtgttg atctccgtgc cgaagtcgta actgcccgcg
     4201 cccgtccgat gcacgttgcg tcggacgggc
//
LOCUS       DASWNS010000366         8362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_223419, whole genome shotgun sequence.
ACCESSION   DASWNS010000366 DASWNS010000000
VERSION     DASWNS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223419"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VGB76_12850"
     CDS             <1..1003
                     /locus_tag="VGB76_12850"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0099828.1"
                     /translation="EGEHSMEMSQLRAFRVVAETLNFTRAAERLHLTQAAISHQIKAL
                     ETELGEPLFIRLKPGVQLSPAGAVALEYAERILDAAEALREQVSGAERALVGRVRAAA
                     ATQAFVHLFAAPFESFMRANPHIELSFRTTVSTEQTLADVLGGAVDVGFGSLPNYSPA
                     LQVTKLFEDELVLVVGGQHRLAPARGDGARGAAAVVAAKEIECERLIMFERGTSIRST
                     TEDFFKRAGIAPALALESNDTYFMKRMIEHGLGISLLPAWAVREEVETGKLAQLEIEG
                     HRLRRSISMISLGRFQPAPTRAFLAFMLDHRAQLQAMASNVELVAATAGAADASTRV"
     gene            1259..2503
                     /locus_tag="VGB76_12855"
     CDS             1259..2503
                     /locus_tag="VGB76_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0099829.1"
                     /translation="MRNVYTFYIDATRIALASIFAHKLRAFLTLIGIIIGVASVVVVG
                     AAISGLNTYVIDKVGKMLGSNHFMIARMASSGQLSEEELERRQRRNPMLNNLDVEWLR
                     QYCTQCTMVGAQAARGTNFDQDGKTFYGAIVFGVTANMGDIEDKTMTDGRFIQQEEDD
                     NASLVVVLGGDIKDKFFPDVDPIGKTFKVGGLPMRVVGVEEKRGPIFGDLVDKHVYIP
                     LRTFERIYGRQQNLQIHGKGSSRDTFQLTIEDARTALRNYHQLSGSEEDDFGLVNTEE
                     LGGQIDEFTGAIAAVVVPITFISLVVGAIVVMNIMLVSVTERTFEIGLRKALGARRKQ
                     ILMQFLIESSTLCALGGVLGLLLAAALAALISATTPIPMTITIGYVLLSILSSGGIGM
                     IAGIYPAFKASRLDPIVALTRN"
     gene            2597..3865
                     /locus_tag="VGB76_12860"
     CDS             2597..3865
                     /locus_tag="VGB76_12860"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0099830.1"
                     /translation="MRLPGLLTKEILAMSLDSIRAHKFRSVLTTIGIVIGVLTAVVIA
                     SILTGLRGNIVRIVEEYGSNNLYAFHLTTGPQVGERDQAERTRKVLTPEDGEALKAAS
                     AVEDVAFEAPNIGYGGGPFDDTISYNGKTYRRGNTKGVTANHAEVINVSLREGRYISE
                     QDDLNRRNVMVIGVNAAEALFPDQPDNIVGKQVKMGGYVFEIIGLLAKRKNTVMGENE
                     EDNAVLIPFRTGLQIAPARKYLILIIRAKSGQTELAVDQVEEILRTRRNVKFNEPNNF
                     DVKTASSFVEQFDSITAMVGVIAIAISSVGLLVGGIGVMNIMLVSVTERTQEIGIRKA
                     VGARRIDIVNQFLFEAMTLTLFGGILGVVLAVLISQIIMFLVPALPASIPMWAVLTGL
                     IVSIGVGLVFGVWPAMKASRLDPIECLRYE"
     gene            complement(3974..4333)
                     /locus_tag="VGB76_12865"
     CDS             complement(3974..4333)
                     /locus_tag="VGB76_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099831.1"
                     /translation="MEFHVDNLKRTVAGMLACGVLLLAGAGLETLAQKPHKEHQKTER
                     RELREHQRYERRTYGTSRTLRRHQKAEKRALKADHKTEKRAYKSERRAQTRGSYINNG
                     RRRYRTNRPIWARARQR"
     gene            4704..>8362
                     /locus_tag="VGB76_12870"
     CDS             4704..>8362
                     /locus_tag="VGB76_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0099832.1"
                     /translation="MSERFYLSLLAVGFACVVTLAPFTDGVTAAGARNLLAANGTETK
                     GAPGEAERALAEGTAHLRRNRADLALPLLESALKLFTGANNRSGLAATHAALGDIYLR
                     HGQYAPAIEHFSRAAEAFRAQHQTANAALMFAKLGETYYLSGDEASARAAFARIGEGE
                     KEHSAAGAASGGGARTSGGSGRRNNAGGGAFNLSTFASMSALLPDACSFALPADGVSP
                     LVNSAANIAANSITPGGNNSPVNPARNPSGSPSNNATGGRPNNPTGRPSTNPSNNATG
                     RPSGNLSNNPANNPTSNPTNNPTSNPSNTNPNNPGGNSTGSSSGSSPNNPSANTAGKQ
                     ANNSPGNSANNPSNNASSNPSGNSPNNPSNSPSNNLSNNPSGNSANNPSGNSSGNSSN
                     NPSNNSANNPSGNSAKKSPNNPSGNSANKPSGKPSGNEPSNMGRAPKKLDDLGRMDLR
                     VFDEQGNPVKGAKARLSTTRPNGIFCDCVNETNAAGRAVLPAIHVGKELKLEVTAPGF
                     EPLEQIVPPEQLAAPFKVTLQTKNAANSIPQPTPKQKFAHAAAPPRRVAATPESASQL
                     ASSAANTLGRAISPNASGACFDFYRLFIAYATRELTLARADFNNNRLDSAQAHYLNVL
                     AAADKNSQAGSLAAARLFRAVSRTSLGDIALRQSRFADALKFYGQAAEIARQDGRAEL
                     TWGAERGLGKTYWVMAAVAADDNAAAKNRNDALKHYRAALGIIETLFAGSLRADDARS
                     NFLATTKDVFDEAAGALAELALMNASPAPALRRQASAHASNANAAPTLEGQSLTYAAE
                     AFRIVEQGKARSLLDMLGDARAEVREGVPAALLERKAANMARQQEIAYLFGGVNLQGS
                     VPPQNVAELEAELERLTVEFNSLENQIRTSSPRYAALVRAQPLTLAEVQRQVLDDSTA
                     LLEYNLGTHQSYLFAATKNGLTLYRLPSRTQIERLAVELRQQLVPAGSRRAIAGINVE
                     STTRGLTLGAPSTAPANVNAYTAAANALYKTVVAPAIAQVGDRRLLVVPDGALNYVPF
                     ESLVASTGGTDYSTLAYLIKTNEIVYAPSASVVAVIRQGARRDVAGRDGAGMLLVADP
                     VFDAGDPRLKSSSLAGAARARESVQRMSLASALSDVTNLKSNNLKLVRLDGTRSEAEE
                     IAKLARANGSKADVWLDFDANEANLHGRRLDNYRVLHFATHGLLDADRPQFTGLALSL
                     VGQTDADGYLRVDEV"
BASE COUNT         1669 a         2628 c         2536 g         1529 t
ORIGIN      
        1 ggaaggagaa cacagcatgg agatgtcgca gttacgcgcc ttccgcgtag tcgccgagac
       61 gctgaacttc acacgcgccg ccgaacgcct gcacctgacg caggccgcca tcagccacca
      121 gatcaaagca ctcgaaaccg aactcggcga gccgctcttc atccgcctca agcccggcgt
      181 gcaactatcg ccggcgggcg cagtcgccct cgaatacgcc gaacgaattc tcgacgccgc
      241 agaagccctg cgcgagcagg tgtccggcgc ggaacgcgcg ctcgtcgggc gcgtgcgcgc
      301 ggcggcggcc acgcaggctt tcgtccatct cttcgccgcg cccttcgaat ccttcatgcg
      361 cgccaacccg cacatcgaac tctcgtttcg caccaccgtc agcaccgagc agactttggc
      421 cgatgtgctc ggcggcgcag ttgatgtcgg cttcggctcg cttcccaact attccccggc
      481 cttgcaggtg acgaagttgt ttgaagacga actcgtgctc gtcgtcggcg gccagcaccg
      541 gctcgcgccc gcacggggcg acggcgcacg gggcgcagcg gcagtggtgg cggcaaaaga
      601 gatcgagtgc gaacgcttga tcatgttcga gcgcggcacc tcgatccgca gcaccaccga
      661 agacttcttc aagcgcgccg gaatagcccc tgcgctcgcg ctcgaatcca acgacacata
      721 ctttatgaaa cgcatgatcg agcacggcct cggcatctcg ctcctgcccg cgtgggcggt
      781 gcgtgaggaa gtcgaaacgg gcaaactcgc gcagttggag atcgaagggc atcgcctgcg
      841 ccgttccatc tccatgatct cgctcggacg cttccagccc gcgcccacgc gcgccttcct
      901 cgccttcatg ctcgaccaca gagcgcagtt gcaggcgatg gcctcaaacg tcgaactcgt
      961 cgcggcgacc gccggagccg cagacgcctc cacgcgagtt tagtgatctc tctcctgagc
     1021 gtcttctgaa aattccatcg ccgccaaacc tgttaataac tcgcgcgcac cgtcgtttag
     1081 agtgtacagc ggagctacgc ctgaggcggc gtcggcgaag ccgtacgcgg cggcatcgtt
     1141 taagtttgcg ccggaattat ctcgcaggcg gctgcgattt atctttaaac cttcggcggc
     1201 ggttcgacgt atatcctcgc gtccagctag gtttcgctcg ccggatcatt ccagtcttat
     1261 gagaaacgtc tatacctttt acatcgatgc cacgcggatc gctctggcct ccatcttcgc
     1321 gcacaaactg cgcgccttcc tcaccctgat cggcatcatc atcggcgtgg cgtcggtcgt
     1381 cgtggtgggc gcggccatca gcgggctgaa cacctacgtc atagataaag tcgggaagat
     1441 gctcggctcg aaccatttca tgatcgcgcg catggcgagt tccggccagt tgtcagaaga
     1501 ggaactggag cgtcgccagc ggcgcaaccc gatgctgaat aatctcgacg tggaatggct
     1561 caggcaatat tgcacgcaat gcacgatggt cggcgcgcag gccgcgcgcg ggacgaattt
     1621 cgatcaggac gggaagactt tttacggcgc catcgtcttc ggcgtgacgg ccaacatggg
     1681 cgacatcgaa gataagacga tgaccgacgg gcgtttcatt cagcaggaag aggacgacaa
     1741 cgcttcgctc gtcgtggtgc tcggcggcga catcaaggat aaattctttc ccgacgttga
     1801 tccgattgga aagactttca aggtcggcgg gttgccgatg cgcgtcgtcg gcgtggaaga
     1861 gaagcgtggc cccatcttcg gcgacctcgt ggacaagcac gtctatattc cactgcgcac
     1921 cttcgagcgc atctacggac gccagcagaa cctacagatt cacggcaaag gctcatcgcg
     1981 cgacaccttc caactgacga ttgaagacgc gcgcaccgcg ctcagaaatt atcaccagct
     2041 atccgggagc gaggaggacg atttcggcct cgtcaacacg gaagaactcg gcggccagat
     2101 cgatgagttc acgggcgcaa tcgcggcggt cgtcgtgccg atcacgttca tctcgctcgt
     2161 cgtcggcgcg atcgtcgtga tgaatatcat gctcgtctcc gtgacggaac gcaccttcga
     2221 gatcggtctg cgcaaagctc tgggggcgcg gcgcaagcag atcctgatgc agtttttgat
     2281 cgaatcttcg acgctctgcg cgctcggcgg cgtgctcgga ctcctgctcg ccgccgcgct
     2341 cgccgcgctc atctccgcca cgacgccgat ccccatgacc atcaccatcg gttacgtcct
     2401 gctctcgatt ctttcatcgg gcggcatcgg catgatcgcc ggaatctacc cggccttcaa
     2461 agcgtcgcgg ctcgacccca tcgtcgcgct aacgagaaat taggcgagaa gttttaggct
     2521 ggaagttgag gacgggaaac ttgagagacg gagtgcggga cgggaagccg ggggcgtgcg
     2581 ccggaggtaa agatttatga gactgccggg attgttgacg aaagagattt tggcgatgtc
     2641 gctcgatagt attcgggcgc acaagttcag gagcgtcttg acgaccatcg gcatcgtcat
     2701 cggcgtcttg acggcggtag tcatcgcctc catcctgacg ggcttgcgcg gcaacatcgt
     2761 ccgcatcgtg gaagagtacg gctcgaacaa cctctacgcg tttcacctga cgacggggcc
     2821 gcaggtcggc gaacgcgatc aggccgaacg cacgcgcaag gtcttgacgc ccgaagacgg
     2881 cgaggctctg aaggccgcga gcgcggtgga agacgtggcc ttcgaagcgc cgaacatcgg
     2941 ctacggcggt ggccctttcg acgacaccat cagctacaac ggtaagacct accggcgcgg
     3001 caacaccaaa ggcgtcacgg cgaatcacgc agaggtgatc aacgtcagcc tgcgcgaagg
     3061 ccgctacatc tcggagcagg acgatctcaa ccggcgcaac gtgatggtga tcggcgtcaa
     3121 cgcggcggag gcgttgtttc cggatcagcc cgataacatc gtgggcaagc aggtgaagat
     3181 gggcggttac gtcttcgaga tcatcggcct gctggcgaag cgcaagaaca ccgtgatggg
     3241 cgagaacgag gaggacaacg ccgtgctcat ccccttccgc acgggactcc agatcgcgcc
     3301 cgccagaaag tatctcatcc tgatcatccg cgcgaagtcg gggcagaccg agcttgcggt
     3361 cgatcaggtg gaagagatct tgcgcacgcg gcgcaacgtg aaattcaatg agccgaacaa
     3421 tttcgacgtg aagacggcaa gctcgtttgt cgaacagttc gacagcatca cggcgatggt
     3481 cggcgtgatc gccatcgcga tctcttcggt cgggctgctc gtcggcggca tcggcgtaat
     3541 gaacatcatg ctcgtctccg tgacggagcg cacgcaggag atcggcatcc gcaaggccgt
     3601 cggcgcgcgg cgcatcgata tcgtcaatca atttttgttt gaagcgatga ccctgacgct
     3661 cttcggcggc atcctcggcg tggtgctcgc cgtgctgatc agccagatca tcatgtttct
     3721 ggttcccgcc cttccggctt cgatcccgat gtgggcggtg ttgacgggat tgatcgtctc
     3781 aatcggcgtc ggcctcgtct tcggcgtgtg gcctgcgatg aaggcttcgc ggctcgaccc
     3841 gattgaatgt ctgcgctacg agtagagcgc ggcgggcgcg tgcggaaaat tttgcggaaa
     3901 aattcaaggc gagcgtcatc aagcgaattg atgacgctcg cctttgttgt ggttcagcgt
     3961 gtgagactaa agtttagcgt tggcgtgcgc gtgcccagat gggacggttc gtgcgatacc
     4021 tgcggcgacc gttgttgatg taggagccgc gcgtttgtgc gcgacgctcg ctcttgtagg
     4081 cgcgcttctc cgtcttatgg tcagctttca aggcgcgctt ttcggccttc tggtggcggc
     4141 gcaaagtgcg gctcgtgccg taagttctgc gctcgtatcg ctggtgttcg cgcagttcgc
     4201 gccgctcggt cttttgatgc tctttgtgtg gcttctgcgc gagcgtttcc agccccgcgc
     4261 ccgccaaaag cagcacgccg caggccagca tgcccgctac cgtccgtttt aaattatcga
     4321 catggaattc catacgtttg cttcctccac tttcatttca ctgtgcaaat tgatgaaagc
     4381 gacgcttgta tgcatcgctc ccgaacctgt tcgggacagc gcgaacgggg aaagcggcgg
     4441 caaactgtat agtctgcgtt cgttgagtcc ggccaaaata gtggtcgcaa ctgttatgcc
     4501 agcgcatccg cgcggcgtgc cgtttacgta atttagagcg agattgtgtg agtctttgga
     4561 ttaggagtcg ggtgtgctaa attcagcgaa cgtgaatgac acgttgatcc ttacggagcg
     4621 gaaatcatgc tgccaaaatt cgattaacgt gagcttgccc tgaaaactcc cctataaatt
     4681 tccgtccatc gccggaggct tagatgtccg aacgctttta tctttcgctg ctcgcggtag
     4741 gttttgcctg cgtagttacg cttgctccct tcacggacgg cgtgacggcg gcgggcgcgc
     4801 gcaacctgct cgcggcgaac gggacagaga cgaagggcgc gccgggtgag gccgaacgcg
     4861 cactcgcaga gggcacggcg catctgcgtc gcaaccgcgc agaccttgcg ctcccgctgc
     4921 tcgaatccgc gctcaaactt ttcacagggg cgaacaaccg gagcggcctc gccgccacgc
     4981 atgccgcgct cggcgacatc tacctgcgtc atggacagta cgccccggcc atagagcatt
     5041 tcagtcgcgc cgccgaagcc ttccgagcgc agcaccagac ggccaacgcc gcgctcatgt
     5101 tcgccaaact cggcgagact tattatctct ccggcgacga ggccagcgca cgcgcggcct
     5161 tcgcgcgcat cggcgaaggc gagaaggaac atagcgcggc gggcgcagca tcaggcgggg
     5221 gcgcaaggac gagcggcggc agcggtcgcc gcaacaacgc aggcggcggc gcgttcaacc
     5281 tctcaacgtt cgcttcgatg agcgcgttgc ttcccgatgc ctgttcgttc gcgcttccgg
     5341 cagacggcgt ttcgccgctc gtcaacagcg cggctaacat cgcggccaac agcattacgc
     5401 cgggcggcaa caattcgccg gtcaatcccg cacgtaaccc gtccggcagc ccgtcgaaca
     5461 atgcaacggg cggtcgcccc aataatccga cgggcagacc gtccaccaac ccgtcaaata
     5521 acgcgacggg cagaccatcg ggcaatctct cgaacaaccc ggcgaacaat ccgacaagta
     5581 acccgacgaa taacccgacg agtaatccgt cgaacaccaa tccgaacaat ccggggggca
     5641 attcaacggg tagttcgtcc ggcagttcgc ctaacaatcc ctcggccaac acggcgggca
     5701 aacaggccaa taattcaccc ggcaactcgg cgaacaatcc atcgaataat gcttcaagca
     5761 atccgtcggg taactcgcct aacaacccgt cgaatagtcc atcgaataat ttatcgaata
     5821 atccgtcggg caattcggct aataacccat cgggtaactc gtcgggcaac tcgtcaaaca
     5881 atccttctaa taactctgcg aataatcctt cgggtaattc ggctaagaaa agcccgaata
     5941 atccttcggg taattcggcg aacaagcctt cgggaaaacc ttcgggcaat gagccgtcga
     6001 acatgggacg cgcgccgaag aaactcgacg acctcggacg catggacctg cgcgtctttg
     6061 acgagcaagg caatcccgtc aaaggtgcaa aggcgcggct ttcgacgacg cgcccgaacg
     6121 gcattttctg tgattgcgtg aacgagacga atgccgccgg gcgcgcggtg ctgcccgcca
     6181 ttcacgtcgg gaaagagttg aagttggaag tgacggctcc cggcttcgag ccgctcgaac
     6241 agatcgtccc gcccgaacaa ctcgccgcgc cgttcaaggt cacgctgcaa acgaagaacg
     6301 ccgcaaactc catcccgcag ccgacaccga agcaaaagtt cgcgcacgcc gccgcaccgc
     6361 cgcgacgagt tgcggcaacg ccggagagcg cgtcgcaact cgcgtccagc gcggcgaaca
     6421 cgctcggcag ggcgatctct ccgaacgctt ccggcgcgtg cttcgacttc tatcgtctct
     6481 tcatcgccta cgcaacgcgc gaactcacgc tcgctcgcgc cgacttcaac aataaccgtc
     6541 tggactccgc gcaggcgcac tacctgaacg tgctcgccgc cgccgacaag aattcgcagg
     6601 cgggaagtct cgccgccgcg cgtctcttcc gcgccgtctc gcgcacgagc ctcggcgaca
     6661 tcgccttgcg tcaaagtcgt tttgcggacg cgctcaagtt ctacggtcaa gccgccgaga
     6721 tcgcgcggca ggacgggcgc gcagagttaa cgtggggcgc ggagcgcggc ctcggcaaga
     6781 cttactgggt gatggccgcc gtcgccgcag acgacaacgc cgccgcgaag aacaggaacg
     6841 acgcgctcaa gcattatcgc gccgcgctcg gcatcatcga gacgctcttt gccggaagcc
     6901 tgcgcgccga cgatgcgcgg agcaattttc tggcgacgac gaaagatgtc tttgacgaag
     6961 cggcgggcgc gctcgcggag ttggccttga tgaacgcttc gcccgcgccc gctttgcgcc
     7021 gccaggcatc ggcgcacgcc tcgaacgcga acgccgcgcc gacgctcgaa gggcagtctc
     7081 taacttatgc cgccgaagcc ttccgcatcg tcgagcaggg caaggcgcgc tcgctcctcg
     7141 acatgctggg cgatgcgcgt gcggaagtga gagagggcgt gcccgccgcg ttgctcgaac
     7201 gcaaggccgc gaacatggcg cggcaacagg agatcgcata cctgttcggc ggcgtgaacc
     7261 tgcaaggcag cgtgccgcca cagaacgtcg cggagttgga agcggaactc gaacgtctca
     7321 ccgtcgagtt caacagtctg gaaaatcaga tcaggacgag tagcccgcgc tacgccgcgc
     7381 tcgtgcgcgc acaacctcta acgctcgccg aagtgcagcg gcaggtgttg gacgattcaa
     7441 ccgcgctgct cgaatacaac ctcggcacgc atcaatccta tctcttcgcg gcgacgaaaa
     7501 acgggctgac gctttatcgc ctgccttcgc gcacgcagat cgagcggctg gccgtagagt
     7561 tgcgacagca gttagtgccc gccggttcgc gccgcgccat cgcgggaatc aatgtggaaa
     7621 gcaccacgcg cgggctgacg ctcggtgcgc cctcgacggc tccggccaat gtgaacgcgt
     7681 atactgccgc cgccaacgcg ctctataaaa cagtcgtcgc tccggccatc gcacaagtcg
     7741 gagacagacg cctgctcgtc gtcccggacg gcgcgctcaa ctacgtgccg ttcgaatcgc
     7801 tcgtcgccag cacgggcggc acagactatt cgacgctcgc ttatctcatc aagaccaacg
     7861 agatcgtcta tgcgccgtcg gcttcggtcg tcgccgtcat caggcagggc gcgagaagag
     7921 atgtcgcagg gcgcgacggg gcggggatgc tgctcgtggc cgaccccgtg ttcgatgcgg
     7981 gcgacccgag gctgaaaagt tcgtcgctgg cgggagcggc gcgggcgcgt gagtcggtgc
     8041 agcgcatgtc gctagcgagc gcgctctcgg acgtgacgaa tttgaagagc aacaacttga
     8101 agttggtgcg tctcgacggc acgcgctcgg aggccgagga gatcgccaaa ctcgcacgcg
     8161 ccaacggcag caaggccgac gtgtggctcg acttcgatgc gaacgaagcg aacctgcacg
     8221 ggcgcaggct ggacaactat cgtgtcctgc acttcgccac gcacgggctg ctcgacgccg
     8281 accgcccgca gttcacagga ctcgcgctct cgctcgtcgg ccagacggac gcggacggct
     8341 atctgcgcgt ggatgaagtc tt
//
LOCUS       DASWNS010000367         7727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_377099, whole genome shotgun sequence.
ACCESSION   DASWNS010000367 DASWNS010000000
VERSION     DASWNS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377099"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="VGB76_12875"
     CDS             <1..1451
                     /locus_tag="VGB76_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0099833.1"
                     /translation="SQTSAQEARERDVQLFQLQWSEDGRHAAMLARSADNKDRWVLLL
                     DPATGKTRVLVALHDDAWTNGPGAFTLGWLPDNRRVYFESEQDGFAHLYTVSIDAGAK
                     PSQLTRGAFEVSDVRLSADKTKFYFTSSEVSFAERHLYSLPIAGGASVRLTSMPGHNE
                     AHISPDERMLAVIHSYSNKPPELYLVGNNAASTARVQVTTSPIPEFFTHNWIDPPLVA
                     IRARDGQTIHARLYKPARQNGAAVLFVHGAGYLQNVHKWWSSYYREYMFHHLLMERGY
                     TVLDIDYRGSAGYGREWRTGIYRHMGGKDLTDHVDAARYLVSEHNIDPKRVGLYGGSY
                     GGFITLMAMFTEPDVFAAGAALRPVTDWAHYNHTYTSNILNLPQSDLEAYRRSSPIYF
                     AEGLRGALLICHGMVDTNVHFQDTVRLTQRLIELRKENWELAPYPVEDHAFERETSWA
                     DEYKRILKLFSEHLHPNSGASPAQQSTGAGNH"
     gene            1598..2029
                     /locus_tag="VGB76_12880"
     CDS             1598..2029
                     /locus_tag="VGB76_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0099834.1"
                     /translation="MNFREAKIEDIPALFEVRYSVKENVLSDPRKVTPEMCVAYLSES
                     GKGWLCEIEGEVVGFSVASLEDTSIWALFVKPSYEGRGIGKRLLRLATDWLFEMRATS
                     IALSTDAHTRADKFYERQGWLRGEIKPDGEVCYRLYKSGRT"
     gene            2362..2817
                     /locus_tag="VGB76_12885"
     CDS             2362..2817
                     /locus_tag="VGB76_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099835.1"
                     /translation="MWLRMLLISLLIGLACLAIIPLSPYTSLVCPAWTIQIVDEAGSP
                     VKGALVTQYWQDYTVESGSHKQVAYADENGYVSFPERNVSASPLSRTWGVIWNTVSLG
                     VHASYGASAHLLAYGKVVDGKRLEGSASYLVGQPLPERLVTRVYDSGCP"
     gene            3011..3445
                     /locus_tag="VGB76_12890"
     CDS             3011..3445
                     /locus_tag="VGB76_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099836.1"
                     /translation="MKITYSLVGGLIGYLIAPLSLAGFGLPLYLNLSLSTQKRLGSIF
                     LCLAILCVLCNAYLRYYRDVHGRGASIFPAVFSLCGIPALLLLRPGSGVWMALSVVAI
                     LVMDFVGQALLGDLIFIKLFPGNLGGGSGTTSGMGEVGSPKR"
     gene            3550..3951
                     /locus_tag="VGB76_12895"
     CDS             3550..3951
                     /locus_tag="VGB76_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0099837.1"
                     /translation="MSQENVETIRRLYEAFGTGDIPQVLGQMEQGIEWNEAENFIYAD
                     RNPYVGPQAILEGVFMRLGTEWDGFKVTPEEWLDAGHRVVVLGTYSGTHKATGKKVRA
                     QFAHIWGVREGRVVRFQQYTDTKQFAEAVVV"
     gene            complement(4176..4589)
                     /locus_tag="VGB76_12900"
     CDS             complement(4176..4589)
                     /locus_tag="VGB76_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0099838.1"
                     /translation="MAQETSRTAPNNHETAAPLKVLIVEDFEDTRFLMRLELEKRGFR
                     VIEATDGEQGVEFALGENPDIILMDIGLPRIDGIEATRRIRHEDSMRDVLIVALTAHH
                     ETEYRAQALAAGCDAYLTKPVDFDWLIDLLGRLLP"
     gene            4931..5332
                     /locus_tag="VGB76_12905"
     CDS             4931..5332
                     /locus_tag="VGB76_12905"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY0099839.1"
                     /translation="MRKRKVAATPRPTDAELEILNVLWQRGASTVRDVHQAITRTRPA
                     MGYTTVLKLMQIMAEKNLVRRDESQRAHVYEAQLEREQTQRQLIGDLLERAFEGSATK
                     LVMQALNTKQASAEELSTIREMLDEFERGDK"
     gene            5329..7419
                     /locus_tag="VGB76_12910"
     CDS             5329..7419
                     /locus_tag="VGB76_12910"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY0099840.1"
                     /translation="MRYTHALLAHPVFQLLGWTLLHFVWQGALVALAYAGIAFSLRRS
                     TANLRYAVACASMFLMLALPVSTFFQLNSKANANAASERERHAAHALEQPVRGDAGAT
                     ERPGVQVRLYANLLVLHDDAGGASAPLQRWAVDRFTSALPWLVLLWLAGALVLTLRLA
                     GGWVLTERLRREPATPLARDWRETLARLARQLRVSRTVRLCESALVEVPTVIGWLRPV
                     ILVPASALTGLSAPQLEALLAHELAHIRRHDYLFNLLQSVVETLLFYHPAVWWLSGRV
                     RVEREHACDDLAVRTTGDVLVYARALTTLETLRRQQQGRANARTLAVAANGGSLMQRI
                     QRLIKTQPATQGHSSLVACLAIISLALASVVVGAQTFSSLQSRDARPSQGDDAAEKSA
                     KVKRQVAVTFVSLPAVGTYDNARAEKETRRLLASLAANNIRTVGFVNEAQLYNEDEGG
                     KLDEERARLLQLWLDAGHELGTQTRDHTSLYKAPLEAFQQDVLRGEEVTGRLMRERGQ
                     PLRFFSYPFLNTGRNRETKEAAERFLAGRGYRIHQVTIDNADWLYGRVYAQARRAEDA
                     ETMRRVADEYVPYLERMFEYYEDLSRDTLGYELPQVLMLHAHALNAHKIDELIAMMKR
                     RGYEFTTLDAALQDKAYAQPTTRTGPWGISWLERWAMERGYKFKPEPRMSDFMRRFDI
                     SQGGTDYNKLPKSE"
     gene            7544..>7727
                     /locus_tag="VGB76_12915"
     CDS             7544..>7727
                     /locus_tag="VGB76_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099841.1"
                     /translation="MSILARKASRLFIGSTLILLTLNFAAHAQPPRRERPATNAARRQ
                     VAVTFDDLPFTGDGGKT"
BASE COUNT         1537 a         2297 c         2310 g         1583 t
ORIGIN      
        1 cttcgcagac gagcgcgcag gaggcgcgcg aacgcgacgt acagcttttc caactgcaat
       61 ggtcggagga tggacgccac gccgccatgc ttgcgcgttc cgccgacaac aaggatcgct
      121 gggtgctcct gctcgacccg gcgacgggta agacgcgtgt attggtcgcg ctgcacgacg
      181 acgcctggac caacggaccc ggcgcgttca cgctcggctg gctacccgac aaccggcgcg
      241 tctatttcga gtcggaacag gacggcttcg cgcacctcta caccgtctcg attgatgcgg
      301 gcgcgaagcc ctcgcagttg acgcgcggcg cgttcgaagt ctcggacgtg cgcctctccg
      361 ccgacaagac gaaattctat ttcacctcca gcgaagtcag cttcgccgag cgtcatctct
      421 attcgctgcc gattgcaggc ggtgcgtccg tgcgtctcac ctcgatgccc ggccacaacg
      481 aggcgcacat ctcgccggac gaacggatgc tcgccgtcat ccattcctac agcaacaagc
      541 ccccggagct ttatctggta ggcaacaacg ccgcctcgac ggctcgcgtt caagtgacga
      601 cttcgcccat ccccgaattc ttcacgcaca actggatcga cccgcccctc gtcgccatcc
      661 gcgcgcgcga cggccagacg atccacgcgc gtctctacaa acccgcacgg cagaacggcg
      721 cagccgtcct tttcgttcac ggcgcgggct atttgcagaa cgtacacaag tggtggagca
      781 gctattaccg cgagtacatg tttcatcatc tcctgatgga gcgcggctac accgtgctcg
      841 acatagacta tcgcggctcg gcaggctacg ggcgcgagtg gcgcacgggc atctatcgcc
      901 acatgggcgg caaagattta accgaccatg ttgacgcggc gcgctatctc gtctcggagc
      961 acaatattga tccgaagcgc gtcggcctct acggcggcag ctatggcggc ttcatcacgc
     1021 tcatggcgat gttcaccgaa cccgacgtgt tcgcggcggg cgcggccttg cgtcccgtca
     1081 ccgactgggc acactacaat cacacctaca cctcgaacat cctcaatctg ccgcagagcg
     1141 accttgaggc atatcgtcgc agttcgccga tctactttgc cgaaggcttg cgcggcgcgc
     1201 tgctcatctg tcacggcatg gtggacacca acgtccactt tcaggatacc gtgcgtctca
     1261 cccaacgcct gatcgaacta cgaaaagaga actgggaact cgccccctac cccgtcgaag
     1321 accacgcctt cgaacgcgaa acgagttggg cagacgagta taagcgcatc ctcaaactct
     1381 tttcagaaca cctacaccct aactccggtg catcgcccgc ccaacagagc accggcgcag
     1441 gcaaccatta accggcagca cggcgcggct ttcggctctc gattgcgtta tgtcaggtcg
     1501 cacagtgcgg cttctcacac ccacgggcaa agttagtcgc accttgcggc acaatatgct
     1561 ttgggttcta ttctctatgg taaggaatta aatctggatg aatttccggg aagcgaagat
     1621 agaagatatt cccgccttgt ttgaggtacg ttactcagtg aaggaaaacg tcttgtccga
     1681 ccctcgcaag gtgactcctg agatgtgcgt ggcttatctc agcgagtccg gcaaggggtg
     1741 gctttgtgag attgagggcg aggtggtggg attctccgtc gcttccctgg aagatacgtc
     1801 aatctgggct ttattcgtga aaccctcgta tgaaggaagg gggatcggta aaagactctt
     1861 gcgactggcg acagactggc tatttgagat gcgtgcgaca agtattgccc tgagtactga
     1921 cgcccacaca cgcgcagata aattctatga gagacagggg tggctgcgcg gtgagatcaa
     1981 gcccgatggc gaggtctgct accggctcta taaatcaggc cgcacctaac acggcatgca
     2041 cccgacacgc gaccagcggc tctttaaatt caaaggcagt agcgggcggg tgatgccgga
     2101 cgttagactc gcttagtaat taaagagaag caaagtctcg cgtggctata ctcagaatgc
     2161 gtatgcatcg ttgaatgatc tcaataatga gctgtctcac cgctcatgca accctaacgc
     2221 actcagcttg ctttttatca tccttctttg gtttcatcca actcgccgct atcgccgggc
     2281 ggcgggttaa ttcgtgcatt agacggctta gctgagtcca tatatcgttg aagaatgagc
     2341 gcggtacctg agcagaggcg gttgtggtta cgcatgctcc tgatcagcct cctgatcggc
     2401 ctggcttgcc ttgccataat tccgctctcc ccctacacgt cgttggtgtg ccccgcgtgg
     2461 acgattcaga ttgttgacga agcaggtagc cctgtgaagg gcgctttggt gacgcagtac
     2521 tggcaggatt atacggtcga gtccggtagt cataagcagg ttgcttacgc ggacgagaac
     2581 ggctatgtga gttttccgga gcgcaacgtg agcgccagcc cgttgtcccg gacgtggggc
     2641 gttatctgga acacagtatc tctgggcgta cacgccagct acggtgcatc ggcgcatctc
     2701 ttggcgtacg gcaaagtcgt tgacggaaag cgactcgaag ggagcgcgag ctatctagtg
     2761 gggcagcctt tgccggagcg gctcgtaact cgtgtctacg attcaggctg tccttgaatt
     2821 gctccggcta cggcgtctga caacggtatg cagcggccag cggtgggcgc gccttttatc
     2881 atccatgtat acaggcgacc gctgatgccg tgcgttatgc gtcacaaata gatcgtttgt
     2941 ttcggaacat tttgtgtggc gagatgtgaa ctgagatgtg agattaacga gttccgggga
     3001 atagtctttg atgaaaataa cgtatagcct cgtcggcggc ttgattggtt atctcatcgc
     3061 tcctctctct ctcgccgggt tcggtctgcc actttatcta aacctcagtc tctctactca
     3121 gaaaagatta ggctccatat tcctatgcct cgctatcctg tgcgtccttt gcaatgctta
     3181 cttgagatat tatcgagatg tacatggtcg gggcgcttca atatttccgg cggtgttctc
     3241 gctctgtggc atacccgcgt tgcttctact gcggcctggt tccggcgtgt ggatggctct
     3301 ctccgtggta gcgatattgg ttatggattt tgtagggcag gcgttgttgg gtgatttgat
     3361 tttcatcaaa ttatttccgg gaaatttagg cggcggctcc ggcacgacat ccggcatggg
     3421 cgaggtcggt tcgccgaagc gttaagcgtg cgcgaatttt tttcttggtg cgggttttta
     3481 gcaacgtatg gcctcttccc ggctataatc cgggcgttca gaaatctcta cggataggag
     3541 gcaggagaga tgtcgcagga aaatgttgaa accatacgcc ggctctacga ggcgttcggt
     3601 acgggcgata ttccacaggt gctcgggcag atggagcagg gcatcgagtg gaacgaggcg
     3661 gagaacttca tctacgcgga tcgcaatcca tacgtggggc cacaggcgat actggaagga
     3721 gtgttcatgc ggcttggcac cgagtgggac gggtttaaag tcacgccgga agagtggctc
     3781 gacgcaggcc atcgcgttgt cgtgctcggc acttacagcg ggacgcacaa ggctacgggc
     3841 aagaaggtgc gcgcgcagtt tgcgcatatc tggggcgtgc gcgaaggccg tgtcgtcagg
     3901 tttcagcagt acaccgatac gaaacaattt gccgaagcgg tcgtcgtcta gcaggcggtc
     3961 gtcgtgtagc agtctgcctc aacacgcttc gcgggcgcgt ctaaagtgat gcccgctcat
     4021 tttacactcc ggcaattttg ccttccggat cgatgatgaa cgtgttgcgg gcgttcgagc
     4081 cgccacctgc tgcgcccgta gcgagcagac agttccacgc ccgcctccgg ctcgaaaccg
     4141 atgaagcgac gaactcatgt cctttgcaga cttcgtcagg gaaggaggcg accgaggagg
     4201 tctatgagcc agtcgaagtc taccggtttg gtgaggtagg cgtcgcagcc tgcggcgagc
     4261 gcctgtgcgc ggtattcggt ttcgtggtga gccgtgagag cgacgatcag cacgtcgcgc
     4321 atagagtctt cgtggcggat gcggcgcgtg gcttcgatgc cgtcgatgcg cgggaggccg
     4381 atgtccatga gaatgatgtc ggggttttcg ccgagcgcaa actcgacgcc ctgttcgccg
     4441 tccgtcgctt cgatgacgcg gaagccgcgc ttctccaatt cgagacgcat cagaaagcgc
     4501 gtgtcctcga agtcttccac gatcagcacc ttgagcggcg cggcggtttc atggttgttt
     4561 ggcgcggtgc gtgaagtttc ctgagccact tgcccctcgt acgaagacta tgtcgtagaa
     4621 aaactctccg gcgtcgaaca cgctcgaaag cgacggatga aatagcgcgt ttagttttca
     4681 acatttcaac gcctgcggtc aacacgcaaa ttgacgcacg gcgaaaaaaa cgcgcctgca
     4741 ccgcccggaa aaaatttctc accctgcgcg gataaacatt catgcccgcg cgcgccagat
     4801 ccgaggccac gcgccccctg ccaaacgttt cgtgatcgaa gtacgaaact tttcgttgac
     4861 attacgaaac gcttcgtagt aaggtgtcga gcctcagggt cgaggacgag tgaagaagga
     4921 gagttacgga atgcgcaagc gaaaggtggc ggcgacgccg cgaccgaccg acgcggagtt
     4981 ggaaattttg aacgtgctgt ggcagcgcgg ggcgagcacc gtccgcgacg tgcaccaggc
     5041 gatcacacgc acgcgacccg cgatgggtta cacgaccgtc ctgaagctga tgcagatcat
     5101 ggcggagaag aatctggtgc gccgcgacga gagccagcgg gcacacgtct acgaggcgca
     5161 gcttgagcgc gagcagacgc agcgacaact catcggtgat ctcctcgaac gcgccttcga
     5221 aggctccgcg acgaaactcg tgatgcaggc tctcaacacc aagcaggctt cggcggaaga
     5281 actctcgacc attcgcgaaa tgctggacga gtttgagcga ggtgataaat gagatacacg
     5341 cacgcgctgc tcgcacatcc cgtctttcaa cttctcggtt ggacgctcct ccacttcgtc
     5401 tggcagggcg cgctcgtcgc gcttgcttat gcgggcatcg ctttctcgct ccgtcgcagc
     5461 acggcaaatc tgcgttacgc agtcgcctgc gcctcgatgt ttctcatgct cgcgctgccc
     5521 gtctcgacgt tcttccaact caactcgaag gccaacgcca acgcggcatc cgaacgcgag
     5581 cggcacgccg cgcacgcgct cgaacaaccc gtacggggcg acgcaggggc gacggagcga
     5641 cccggcgtgc aggtgcgtct ctacgcgaat ttattggtct tgcacgacga tgcgggaggt
     5701 gcttccgcgc ccttgcagcg ttgggctgta gatcgtttta cgtccgcttt gccgtggctc
     5761 gtgttgctct ggctggcggg cgcgctcgtg ttgacgctgc gccttgcggg cggctgggtg
     5821 ttgaccgagc gactcaggcg tgaacccgca actccgctcg cgcgcgactg gcgggagaca
     5881 ctggcgcgac tcgcgcggca gttgcgggtt tcacgaacgg tgcgtctgtg cgagtctgcg
     5941 ctggtcgaag tgccgaccgt gatcggctgg ttgcgtccgg tcatcctcgt ccctgcgagc
     6001 gcgctcacgg gtttgagcgc gccgcaactc gaagccctgc tcgcgcacga actggcgcac
     6061 atccgccgcc acgattatct cttcaatctc ttgcagagcg tggtggagac gcttctcttc
     6121 tatcacccgg cggtgtggtg gctctccggg cgcgtgcgcg tcgagcgcga gcacgcctgc
     6181 gacgacctcg ccgtacgcac gacgggcgac gtgctcgtct atgcgcgcgc actgaccacg
     6241 cttgagactc tgcgccgcca gcagcagggc agggcgaacg cccgcacgct ggccgtcgcc
     6301 gccaacggag gttcactcat gcaacgcata caacgtctga tcaagacgca gcctgcaacg
     6361 cagggtcact cgtcgcttgt cgcctgtctc gccatcatct cgcttgcgct ggcaagcgtc
     6421 gttgtgggcg cgcaaacttt cagttcgctc cagagcaggg atgcgcgccc ctcgcagggt
     6481 gatgacgctg cggagaagag cgcgaaggtg aaacgacagg tggcggtcac gttcgtcagc
     6541 ctcccggcag tcggcactta cgacaatgcg cgtgcggaga aagagacgcg ccgcctgctc
     6601 gcgagcctcg ccgccaacaa catccgcacg gtcggcttcg tcaacgaagc ccaactttac
     6661 aacgaagacg aaggcggcaa gttagacgaa gaacgcgcgc gcctgctgca actctggctc
     6721 gacgccgggc acgaactcgg cacgcagacg cgcgaccaca cgagtctcta caaggctccg
     6781 ctcgaagcgt ttcagcagga cgtgttgcgt ggcgaagaag tgacgggcag gttgatgcgc
     6841 gagcgcgggc agcctttgcg tttcttcagt tatccctttc tcaacacggg gcggaaccgc
     6901 gagacgaaag aggccgcgga gcggtttctc gcggggcgcg gctaccgcat tcatcaggtg
     6961 acgattgaca acgccgactg gctctacggg cgcgtctatg cgcaggcgcg cagggcggag
     7021 gacgctgaga cgatgcggcg cgtcgccgac gagtacgtgc cttacctgga gcgcatgttc
     7081 gagtattacg aagacctctc gcgcgacacg ctcggctatg aactgccgca agttctcatg
     7141 ctccacgcgc acgcgctcaa cgcgcacaaa attgacgaac tcatcgcgat gatgaagcgg
     7201 cgcggctacg agttcaccac gctcgacgcg gccttgcagg acaaagccta cgcgcagccc
     7261 accacccgca ccggaccctg gggcatctca tggctcgaac gctgggcgat ggagcgcggg
     7321 tataaattca aacccgaacc ccgcatgtcc gacttcatgc gccggttcga catctcgcag
     7381 ggcgggactg actataacaa gctgcccaag tccgaataac cgacgccgcg ccccaagcga
     7441 aaaggctgtc gcttcgtccc ggcagccttc tcgccaaact tacgcgtacg cgctcgtcta
     7501 tactctccaa ccgaaatcaa tcatccgata aggggaattt tttatgagta ttcttgcccg
     7561 caaagcgtcc cgacttttca tcggttcaac tttaatcctg ctgacgctca atttcgccgc
     7621 gcacgcgcag ccgccgcgac gtgagcgtcc ggcgacgaac gccgcgcgcc gtcaggtcgc
     7681 cgtcacgttc gacgatctgc cgtttacggg cgacggaggg aagaccg
//
LOCUS       DASWNS010000368        22429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_15134, whole genome shotgun sequence.
ACCESSION   DASWNS010000368 DASWNS010000000
VERSION     DASWNS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22429
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15134"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /gene="priA"
                     /locus_tag="VGB76_12920"
     CDS             <1..1139
                     /gene="priA"
                     /locus_tag="VGB76_12920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY0099842.1"
                     /translation="PSLESFHNARSGKYRYLRLPNRVANRAMAVAEIVDMREVFEREG
                     RQQALSPQLLDAIEETHARGEQSIVLLNRRGYSSFVLCRSCGERMQCPECDVTLTYHQ
                     RERSLICHYCDHRRRPPTECPACKGKYIYFIGEGTEQIEEIIKGRFPTLRIARLDRDT
                     TARRHTYEEAIMRFGSGELDMLVGTQMIAKGHDFPNVTLVGVVSVDAGLAMPDFRASE
                     RTFQLITQVAGRAGRGSLAGRVLIQTFHPEHYALRHACAQDYDAFYEEEIRYRRDLSY
                     PPFVSLASLLVHGEDLTRVQTTATEIRLALDRANAERACRILGPAPAPLARLRGEHRF
                     QILLKARNRTRMRSLLDLALAEAGASRNCDLHSVNVEIDPVNLM"
     gene            1211..2248
                     /gene="lpxD"
                     /locus_tag="VGB76_12925"
     CDS             1211..2248
                     /gene="lpxD"
                     /locus_tag="VGB76_12925"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY0099843.1"
                     /translation="MRLSELASLTKASIEGAPEDVEITGGAGLDEAEAGHVTFLANPR
                     YTRRMQTTRASAIYLAEGVEAGRTDIVVLRTRDPYLAYTRALRLFHPEPEFESFIHPS
                     AIIDPSARVSERVWIGACVVVGREVEIAEGVRLFPHVTIYDNVRIAENSTIHSGVALR
                     AGTQIGARVTIHNNVVVGSDGFGYAKDEERRWLKIPQTGRVCIEDDVEIGAGTTIDCA
                     SVGETRIARGTKIDNLVQIGHSCTVGEDTLLCAQVGLAGSSHIGNRVILAGQAGVAGH
                     LTIGDDVVLMAKSATSHDVPAGKVLSGIPAFDNRDWLRAIAAFRRLGDMQRTVRQLEA
                     RLAALEKSGDD"
     gene            2556..2858
                     /locus_tag="VGB76_12930"
     CDS             2556..2858
                     /locus_tag="VGB76_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099844.1"
                     /translation="MKSSNRAATATPHVLPSNREDQQVEVVIEERDGQQGVALRLSTW
                     TEGLGWCCQKTIRLDGEQLDDLHHAISVARHRLNRQRVEDGQFRPSAQVVQLPAIG"
     gene            complement(2945..3406)
                     /locus_tag="VGB76_12935"
     CDS             complement(2945..3406)
                     /locus_tag="VGB76_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0099845.1"
                     /translation="MANANLIESLNRALSLEIGAVIQYTQHSFLVTGIEREVYRKYFR
                     KQGEESQEHAITLGDKIVALGGVPTVEPAMIRQSTELAEMLRQDLEMEREALKAYMAA
                     WEACGEEDRPTRFQLEERIYNEQLHVEELEKLTSERKANVTKERITLKQVG"
     gene            complement(3649..4398)
                     /locus_tag="VGB76_12940"
     CDS             complement(3649..4398)
                     /locus_tag="VGB76_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0099846.1"
                     /translation="MSLLLEGKRAFISGGTRGIGAAMCEIFAREGADVAFNYHTREDL
                     AETMAQKIKAHGRRAHGFKVSVTDRVGMKRLTRQLVEEWGGVDVLVNNAAINRGDNFA
                     TTTDRAWDEVIDTNVGSLFAVTKPIYKQMIRQRKGAILNITSIGAIRSLPTSVHYATS
                     KAAMIGFTKCLSREAANFNVSVNAIAAGIFDTELAHTLPPHLLEMHKGWSPRGELGRP
                     EELAEFAAFMVSDRNSFMNGEIVIIDGGSVT"
     gene            complement(4548..5750)
                     /locus_tag="VGB76_12945"
     CDS             complement(4548..5750)
                     /locus_tag="VGB76_12945"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099847.1"
                     /translation="MFRARVPKILIISSDTGGGHRSAAVAIAAGLQKFFKGNSYAVRV
                     VRAIEESHSISAKLVNVYNWVLRHRQHWMKYLYWAVNRFRPETREFFYRRSVGYVHEL
                     FERWCPHVVVSVHPLTQHAIGRVLRELKLADKIPLVTVVTDPCYGFWRGWACDDVRLY
                     LVASEEARQQLIDYGVAPERIKISGMPVHPKFNLPDELAAQAARNALGLDPQKFTVFV
                     NAGWVGGGNIPEIFRELVRGDLDVQAIFLAGNNKELRAEAELVAARAKFPVKVIGYSE
                     HVEQLMQAANVMISKLGGLTTFEALACRVPIIADATTEPMPQEAGAATMLTKRGAGVL
                     LKRTSDIVPAVRRMVEDTRYYAAMRTATAGLAIPHSTRRIVEEITALIPAPVLAEEER
                     VVADVHIA"
     gene            complement(5844..7007)
                     /locus_tag="VGB76_12950"
     CDS             complement(5844..7007)
                     /locus_tag="VGB76_12950"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0099848.1"
                     /translation="MDYTTKELLKRYAKLALGQPFAPVLLNILVTSVCDMRCTHCFFT
                     DELNDRPRKKLQMKTHEIQRVSETLGGNLGVLILAGGEPFTRKDLPEIVRAFYENNKL
                     ESVYLMSNGQIQKRILPDVTRILEECPNLNVAVALGIDGLKDQHEKIRQKPGSWDIAI
                     DTARELQKIKREYPRLDIQTCTCFMNTNQDTIFEWYDFLKYDLKPDKVNFNYIRPPSA
                     DPKELDIDHARYAELAHMIDSDSRQSAIKNNYGGDAGFFKAAIDIYMHGLIAKAQEEQ
                     QAQMTCYAGTAGGVIYDEGTVSSCENLDAVGNLRDYDWNFQKLWLSPAMQERRRKAKS
                     GCFCTHESNSYYPSLPFNPKHLIQIKRLEREMKKSRKQIERDEARAEGLTVKA"
     gene            complement(7151..8518)
                     /locus_tag="VGB76_12955"
     CDS             complement(7151..8518)
                     /locus_tag="VGB76_12955"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0099849.1"
                     /translation="MSDSILIVDDERGIRETLGGVLEDEGFTVDAVASGEECLQAIER
                     RAYSCVLLDVWLPGIDGLETLSRLAATGADSAVVMISGHGNIETAVRATKLGAFDFIE
                     KPLSIERTILTVRNALRQRALERLNATLAAEMNEEHAMVGESVAMRALRKQIAAVAPT
                     DGRVLVYGESGTGKELVARAIYAQSRRAGAAFIELNCAAIPEELIESELFGHVKGAFT
                     GATAAKRGKFELADGATLFLDEVGDMSARTQAKVLRVLEEQRFAPVGSGAQIRVDVRV
                     VAATNKRLEDEIERGNFRADLFYRLNVIPFEVPPLRDRLEDVPLLVEHFNRKFSTAYG
                     KPAKRFELEAMDALQSYTWPGNVRELRNTVERVVIMTGKPKVNASDLPPLGGEAPPVA
                     VYRFNSFKEASEAHQREFIRRKLAEAEGNVTRAAELMGMDRSHLYRRMRALGVKVRGE
                     RENGN"
     gene            complement(8518..11049)
                     /locus_tag="VGB76_12960"
     CDS             complement(8518..11049)
                     /locus_tag="VGB76_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099850.1"
                     /translation="MSNPDAPTPEELNEPKPKRRSAPWLLGSLVLSLLFVLVALQVFG
                     LWEVFTPDTAADTLLLYALSSLNFVAFIVFAFIFVRNLLKLRRERRAHELGSKIKTRL
                     VVYFITVSLLPITVMALFSYFFLNRSLEKWFGRLPEDVQRVARDAQREDMDEQVRHLL
                     EQTSLVAKQLERDAIQNVATPQTANTQAAGATQGVAPGNAATTQNPATAATTESAASN
                     VAQASLAQWSDGGQFAALQLIAADGSVIAQTIAPQFAARAGELEPELSRVRAAEGGSG
                     AFSNKFTVAKVSLADGRALVAARASRVKTSLERIVAGSETFEQLRREQRKVRSLGLST
                     IGLMTLLLLFAATWSAIHLARGIGAPIRALAEAADEVARGNLAHRVTAIADDELAILA
                     ASFNQMTAQLEENRRRIEAGAGELREKNLALDERRNYIETVLESLSTGVISLDEENRV
                     TTINAAAVAMLRLGESPAAGAPLKEMLSMEDHLVLERVLTRARRTGRAAWQMELARGA
                     SIDGGVVPVALTATALGNFTEGGARARSSRGIVLVIEDLTELLAAQRAAAWSEVARRM
                     AHEIKNPLTPIQLSAERIARNFRRMRNGSQPAHEDASNGEAVSSSGDANRTVEPELVE
                     QARVAQVVEECTATITREVAGLKAMVDEFSRFARLPHARLEAADLNEVVRQAVALYED
                     RLEDVRLDVHLARTLPPGLLDAEQMRRAIVNLIDNALEAMTDATGERRITVATGHDPA
                     RAMLLIEIGDSGHGIERPDFPRLFQPYFSTRGRGTGLGLAIVQRIINEHGGRIRAEPN
                     RPRGAKFLVEIPVTDETHKPNDERGTMNDESVSSSSFIVHPSSLS"
     gene            11196..12152
                     /locus_tag="VGB76_12965"
     CDS             11196..12152
                     /locus_tag="VGB76_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY0099851.1"
                     /translation="MTPLDIFLNRHGRLRSGWRLAIFCAAYYAALAAIFTTIYAVFFL
                     LKIPNVDARLAGTLGYILQSFILLSTALVVGWACGRLFEDLPLRALGWARHRGWLRDL
                     LLGTLLGIASLMLAALLATVFRGFQFSVTPTISSVAFLKTFFGSAVIFIFAAAGEEML
                     FRGYPLQTMMRALPFIVAILPSSVLFAYVHLNNPNVSWGFTFINTTLAGFWLAVAYLR
                     TRSLWFPLGLHWAWNWAMGALLGLPVSGITSLTRAPLFRATDTGPAWLTGGAYGIEGG
                     AACTVALIISTIFIWKTGLLKADEELKKFTDGEQTKKVMSDE"
     gene            complement(12247..13458)
                     /locus_tag="VGB76_12970"
     CDS             complement(12247..13458)
                     /locus_tag="VGB76_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY0099852.1"
                     /translation="MENAKEAATAPATETPKEASTPAAAAPAAAAAGGGAGLRGVVAA
                     SSSIGDVNGEQGILIYQGYDIHDLAEHSNFEETVYLLWHGRLPTQTEFDEFKRAIGEH
                     YELPPQVIELIRQFPREAEPMDMLRTAVSALDFYDKECCDISREGALRTATRLTAQFP
                     VLVAAIERLRQGQEPIPANPELDIATNFLYMLKGEMPTEREARIFDICLILHADHELN
                     ASTFTARVVAGTLADVYGAVTAAIAALSGPLHGGANTNVMKMLLQIGEVDKAEDFVRD
                     ALARKQKIMGIGHAVYKTEDPRATHLRKFSQEVGESKGETKWYEISRKIEEYIHREKG
                     MYPNVDFYSASTYYMMGIPLELYTPIFAVSRISGWTGHVLEQYANNKLIRPRAEYIGQ
                     RDLKYVPINER"
     gene            complement(13625..14431)
                     /locus_tag="VGB76_12975"
     CDS             complement(13625..14431)
                     /locus_tag="VGB76_12975"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEY0099853.1"
                     /translation="MSIITLLTDFGTADYFVGAMKGVILSLDPQAHIVDITHEIPAHD
                     IEAGAFTLLAAYGSFPPQTIHVAVVDPGVGSARRPLLIAAHGHFFVGPDNGLFSYVCE
                     GATDARVYHLTNENFFRTQVSATFHGRDIFAPVAGALSRNVPPSALGELIDGEMRRLA
                     PLAPLREGDGAWSARIIHIDRFGNLVTNITRRELTDEQLAEGRKLEIGGREISSFRRF
                     YAEAAGAPPGELFAIWGSAGLLEIAADRASAAQLLNASRGQLVRVSGRKL"
     gene            complement(14540..15598)
                     /locus_tag="VGB76_12980"
     CDS             complement(14540..15598)
                     /locus_tag="VGB76_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099854.1"
                     /translation="MQIRKRLLPASVSFALAVALLPTTFMSRAAASDNTDAPVVAVQQ
                     VGALERGYRTGYSDGYQAGYRDSIERAARDYRNKEDYVSADRAYIPAYGAREDYRDGY
                     QQGFESGYTAGFDRRGFDSVVPPGLSRRGAAPSNDVGRGVNDKVGGTSDSMPDPRASD
                     SNSTSGGAVRQIGSLDLPMDTTMRIELLTNLSTDATQRGDRFEARVIEPAQYEGARIV
                     GRVKGVKRPGKVRGSGELQLDFEQISLPDGRFTNLNAQVIEVVGQSTGNVGEVDEEGG
                     VRGQSTTKDDVTKVGAGAGIGAIIGAIAGGGKGAAIGAVIGGSIGAGGAITSRGKDVR
                     LQRGQELLIRTSTETRIQ"
     gene            16042..17259
                     /locus_tag="VGB76_12985"
     CDS             16042..17259
                     /locus_tag="VGB76_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY0099855.1"
                     /translation="MRRVYLDNSATTPIDPRVVEAMLPYLTEKFGNASSVHFYGQEAR
                     VALDRARREVAALINARPNELVFLSGGTEANNLAIRGIAETAAEHGRHIITSQIEHSA
                     VRGVCDALERRGWEVTRLPVYADGIVRLEDVRAALRPDTVLITLMLANNEIGTIQPVG
                     EVGALVREEREQRGRKYLWLHTDAVQAAGRTTVDVDALGCDLLSLSAHKLNAPKGVGA
                     LYVRRSVRLTPQSIGGHQERERRAGTESVPLIVAFGKAAALAREELDSRIAHLSRLRD
                     QFERGVEASVAEIVFNGDRARRLPHLSNISFRFIEGEGLLINLDMQGVAVSTGSACSS
                     GSLEPSPVIRAIGRDDELARGSIRFSLGKETSEEEIAYVLEVLPRAVENLRRLSPLYQ
                     RALATRACETTNA"
     gene            17348..17728
                     /locus_tag="VGB76_12990"
     CDS             17348..17728
                     /locus_tag="VGB76_12990"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEY0099856.1"
                     /translation="MPYSEAFKDHLAHPRNGGELADADASAELTNPACGDRLRLTLRI
                     RDGRISAARFLAYGCPPTLACGSALTEMLEALTIAEAAGITRQQLTHALGGLPARKSH
                     AAALAIETLHAALNDRRLLTESSE"
     gene            17813..18385
                     /locus_tag="VGB76_12995"
     CDS             17813..18385
                     /locus_tag="VGB76_12995"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0099857.1"
                     /translation="MSRQPQTYITPGEYLALERQTEYKHEYFDGEIFAMVGASRKQNL
                     ITVNITRELSQQLKGKPYEVYSSDMRIRIPNANIYTYADLTVACGEPKFEDAQVDTLL
                     NPVLIVEVLSKSTASYDRRQKFAFYRTIESLSDYILVSQESFHVEQYTKQTDNRWLLS
                     DFRSMEDALQLDSVQCSLALREIYDKVSLS"
     gene            complement(18461..19837)
                     /locus_tag="VGB76_13000"
     CDS             complement(18461..19837)
                     /locus_tag="VGB76_13000"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0099858.1"
                     /translation="MMLQRRWLTALFCTLALLTNGAGALLQAQTQQRTRQAPPPTPEH
                     DVIVGREAVLVPHQPSGEPFEMHIAPGAPNNSFNFSFMSSEMHFDSKIVKGAPYSAEA
                     VTESVQTLADGNRITRTSKASLYRDSEGRTRRDQTLNHIGPWATADEPPQTIFINDPV
                     SGAHYVLNPRTRTAQKMPNMFFFTRTREEVNELHPAPGEARTQVLVRSGGSADVVVSS
                     APLKAADPGLSGKAIKKVQPTYPAIAKAAGAQGSVTVQIVINEAGEVESAKANSGHPL
                     LQQAAVDAAKEWRFTPTKLSGNAVKVSGMISFVFALPKGEDAPTSEVSFLRTPATGHA
                     PEGDGLQVRVAVPKPVTESLGKQNIEGVEAEGTRTTVTFPAGTFGNERPINVVSERWY
                     SPELQTVVMTKHSDPRFGETNYRLTNIDRSEPARTLFEVPSDYTLKAEESHPRRVRMM
                     TPGKVRQQ"
     gene            complement(19948..20922)
                     /locus_tag="VGB76_13005"
     CDS             complement(19948..20922)
                     /locus_tag="VGB76_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099859.1"
                     /translation="MNCQQFTSFIVEVARGQMMDAASRDGAVRHAESCQACAARLAQE
                     QSLTGALRAVAQDIEDLSAPARVEAKLLAAFHESLPQRHAPATATVASTPAPVSQLTR
                     DARTTPDASRFSRRSVFNATAAIAASLVLVVLATLYLRFTQTAPPTPQPLATNRQTVA
                     GKASPPDRELTLAASPSPAPSSAPSIEAKPTPERAMPDAFKGVQSPRTNVKVASRQRR
                     VPTMIKAQHVIDGGHAIIEPGVEGEMALNGAAKPGEAESVTDFISLVADAPAAPPLES
                     GQLVRVQVPRAALASLGLPLNAERGNEPVKADVLLGGDGLARAIRFER"
     gene            complement(20919..21557)
                     /locus_tag="VGB76_13010"
     CDS             complement(20919..21557)
                     /locus_tag="VGB76_13010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0099860.1"
                     /translation="MKASPEHSDEELLRLILAGDADAFTALYRRRQAGIYRFALQMCG
                     SEGIAEDVTQEVFMTLVRREAQIFDASRGTLAAYLYGIARNQVLRRLEKERHFVALVE
                     ETAEDGGAATATQHPATTGHDPLAELTRHELIEAVQQAVLALPAHYREVVVLCELHEM
                     SYAEAAAALNCAVGTVRSRLHRARALLCERLREVQRREAGVAEDFDPLRCFA"
     gene            complement(21722..>22429)
                     /locus_tag="VGB76_13015"
     CDS             complement(21722..>22429)
                     /locus_tag="VGB76_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0099861.1"
                     /translation="LSYTLLVDNSGSLRTMFDDVLKAGGAIVAGMRPQDEMSIVRFVS
                     RDKIELLQNFTGSKNMLAAALDEMYLEGGETALLEALYVTAETLTTRTPGESRHRALV
                     VITDGGERDPRSKLDELLTLLRRQHVHVFIFGLTGALSDDAFRQVKGGRRKSRELLET
                     LARETGGRAVFPKKTSEFATTAAALNRELQMPEYVLGYTPGNTTNTGKPSVVQLKLAN
                     SALSRGKLQLHTSFQSR"
BASE COUNT         4083 a         7021 c         6817 g         4508 t
ORIGIN      
        1 cgccctcgct cgaatctttt cacaacgcgc gcagcggaaa gtatcgctac ctgcgcctgc
       61 ccaaccgcgt cgccaatcgc gcgatggccg tggccgagat cgtagacatg cgcgaagtgt
      121 tcgagcgcga gggacgacag caagcgctct cgccgcaact gctcgacgct atcgaagaga
      181 cacacgcgcg cggcgagcag tcaatcgtgc tcctgaatcg tcgcggctat tcgagcttcg
      241 tcctgtgtcg ctcgtgcggt gagcgcatgc aatgccctga gtgcgacgtg acgctcacgt
      301 atcaccagcg cgaacgcagc ctcatctgcc actactgcga ccaccgccgc cgaccgccga
      361 ccgaatgccc cgcgtgcaaa ggcaagtaca tctacttcat cggcgaaggc acggagcaga
      421 tcgaagagat catcaaggga cgcttcccta cgctgcgcat cgcgcgtctc gaccgcgaca
      481 ccaccgcgcg ccgccacacc tacgaagagg ccatcatgcg cttcggctcc ggcgaactcg
      541 acatgctcgt cggcacgcag atgatcgcca aaggtcacga cttcccgaac gtcacgctcg
      601 tcggcgtcgt ctccgtcgat gcgggactgg cgatgcccga ctttcgcgcg agcgagcgca
      661 cgttccagtt gatcacgcag gtggcgggtc gcgccggtcg cggctcactc gccggtcgcg
      721 tcttgatcca aacttttcat cccgaacatt acgccctgcg ccacgcctgc gcgcaggatt
      781 acgacgcgtt ttacgaagag gagattcgct atcgcaggga cctgagctac ccgcccttcg
      841 tctcgctcgc atcgctcctc gttcacggcg aagatttgac gcgcgtgcag acgacggcga
      901 cggagatcag gcttgcgctc gaccgcgcca acgccgaacg cgcctgtcgt atcctcggcc
      961 ccgcgcccgc cccgctcgca cggctgcgcg gcgaacatcg cttccagatt ttgctcaaag
     1021 cacgcaaccg cacgcgcatg cgttcgctcc tcgacctcgc gctcgcggag gcgggcgcgt
     1081 cgcgcaactg cgatctgcac agcgtcaacg tagagatcga tccggttaat ttaatgtagg
     1141 agaggcgcgc gtccggaagt tgaagcggcg cttgaagcgg aggttgaacc ggaagcgcgc
     1201 gattttgttg atgagacttt cagaattagc cagcctgaca aaagcgtcca tcgaaggcgc
     1261 gccggaagac gtagagatca cgggcggagc cgggcttgac gaggccgaag ccggacacgt
     1321 caccttcctc gccaacccgc gctacacacg gcgcatgcag acgacgcgcg cttccgccat
     1381 ctatctcgcg gagggcgtcg aagcgggcag gacggacatc gtagtcctgc gcacgcgcga
     1441 cccttatctc gcctacacgc gcgcgttgcg tctctttcat cccgaaccgg agttcgagtc
     1501 gttcatacac ccttcagcca tcatcgatcc ttcggcacgc gtcagcgagc gcgtgtggat
     1561 cggcgcgtgc gtcgtcgtcg ggcgcgaggt cgagatcgcg gagggcgtgc gcctttttcc
     1621 gcacgtcacg atctacgaca acgtacgcat cgccgaaaat tcgacgattc actccggcgt
     1681 cgcgctgcgc gcgggcacgc agatcggggc gcgtgtcacg attcataaca acgtcgtcgt
     1741 cggttcggac ggcttcgggt acgcgaagga cgaagaacgc cgctggctca agattccgca
     1801 gacggggcgc gtctgcattg aagacgacgt ggagatcggc gcgggcacga cgattgactg
     1861 cgcctccgtc ggcgagactc gcatcgcgcg cggcacaaag attgacaacc tcgtgcagat
     1921 cggccactcg tgtaccgtcg gcgaagacac gctgctctgc gcacaggtcg gactcgccgg
     1981 aagttcgcac atcggcaacc gcgtcatact cgcggggcaa gcgggcgtgg ccgggcatct
     2041 caccatcggc gacgacgtgg tgctcatggc gaagagcgcg accagccacg atgtcccggc
     2101 gggcaaagtg ctctccggca tccccgcctt cgacaatcgc gactggctgc gcgccatcgc
     2161 cgccttccgc cgcctcggcg acatgcagcg caccgtgcga caactggaag cacgcctcgc
     2221 cgcgctcgaa aaatccggcg acgactgacg aataacgaat tatcgagcct ctctaagaat
     2281 ttaagactca taacagagac gcggaggacg cagagctggc gcagagatca ttcaattctc
     2341 tgcgccaact ctgcgtcctc tgtgtctctg cggtgaattt ttatccctac cgaatcccaa
     2401 attgcacaaa aacccgctca cgctaaattt tatcttgaca tccgggcggg ctgacgccga
     2461 taataggttt tgaaattgga tttcaatttc gccgccaggt atcgaaatcc aatcgaatca
     2521 cagctattac tgatacttct taggagtcta agccagtgaa atcctcgaac cgtgccgcga
     2581 cagcgacccc gcacgtcctc ccttcaaacc gcgaagatca acaagtcgaa gtcgttattg
     2641 aagagcgaga cgggcagcag ggcgtcgcgc ttcgtctctc cacctggacg gaggggcttg
     2701 gctggtgttg ccagaagacc atccggctcg acggcgagca acttgacgac ctgcaccacg
     2761 ccatcagcgt cgcgcgccat cgcctgaacc ggcagcgcgt ggaagacggg cagttcaggc
     2821 cgtcggcgca ggtcgtccaa cttccggcca tcggctgaga tttaaccatt atcaacgtaa
     2881 aaaggcagcg cgctggatcg tccagcgcgc tgccttttta tttcgggcga ttcccttttc
     2941 cgtctcagcc gacctgtttg agcgtgatgc gctctttcgt cacgttggcc tttcgctcgg
     3001 aggtcagttt ctccaattct tcgacgtgaa gttgctcgtt gtagatgcgc tcttcgagtt
     3061 ggaagcgcgt cgggcggtct tcttcgccgc aggcttccca ggccgccatg taagccttaa
     3121 gagcttcgcg ctccatctcc aaatcctgcc gtagcatctc cgcgagttcg gttgactggc
     3181 gaatcatggc cggttcgacc gtcggcacgc cgccaagcgc gacgatctta tcgccgagcg
     3241 tgatggcatg ctcctgcgat tcctcgccct gcttgcgaaa atatttacga tagacttcgc
     3301 gctcgatgcc cgtcaccaga aaactgtgct gcgtgtactg tatgaccgcg ccgatttcca
     3361 gactgagcgc gcgatttaac gattcgatca aatttgcgtt tgccactgac aagcctcccg
     3421 ctaaagtttc cgccgaatgt aaaagtgctg gcggatgtta ccgcgcgaag ctcaagctgt
     3481 caagataact attgaaatag ttaaacttaa caatgaaatc cattttcagt tgcaaaaata
     3541 aggaggcgag tctcgaatgt agttggcggg tgcgttgtga gtattgcaga tggcttcgat
     3601 aaaagcctgc gatggcgttt gggcggaagc ggcgtcggaa ggcgcgggct acgtcaccga
     3661 gccgccgtct atgatgacga tctcgccgtt cataaaactg ttgcggtcgc tgaccatgaa
     3721 ggcggcaaac tccgccagtt cttcggggcg tccgagttcg ccgcgcggcg accaaccttt
     3781 gtgcatctct aacaggtgcg gcgggagcgt gtgtgcgagt tcggtgtcga agattccggc
     3841 ggcgatggcg ttgacggaga cgttgaagtt ggctgcctcg cgcgagagac acttcgtaaa
     3901 accgatcatc gcggccttcg aggtggcgta atggacggag gtgggcagcg aacggatcgc
     3961 gccgatggaa gtgatgttga gaatcgcgcc tttgcgctgg cggatcatct gtttgtagat
     4021 cggcttcgtc acggcgaaga ggctgccgac gttggtatcg atcacttcgt cccacgcgcg
     4081 gtcggtcgtg gtcgcaaaat tatcgccccg gttgatcgcc gcgttgttga ccagcacatc
     4141 gacgccgccc cactcctcca cgagttgacg cgtcagacgc ttcatcccga cgcgatccgt
     4201 gaccgagact ttgaaaccgt gcgcgcgtcg cccgtgagcc ttgatctttt gcgccatcgt
     4261 ctcggccagg tcttcgcgtg tgtgatagtt gaaagcaacg tccgcgcctt cgcgtgcgaa
     4321 gatttcgcac atcgccgcgc cgatgccgcg cgtgccaccg gagataaacg ctcgtttccc
     4381 ttccagcaaa agactcaact tggccgctct ccaaaaaagc ccgcttcctt tttactcgga
     4441 gcgggcgaga tgtaatgaat gctgaattaa agcctgaacc gcgtgcgggc gtcaaccgcg
     4501 tgcgcgcacg tcaaactctc gcgggcgaga actatccggc gcgcaactca agcgatgtgc
     4561 acgtctgcaa ccacgcgctc ctcttccgcc agcacgggtg cggggatcag cgcggtgatc
     4621 tcttccacga tccggcgcgt cgaatgcggg atagccagac cggcggtggc cgtacgcatc
     4681 gccgcataat agcgcgtgtc ttcgaccatg cgccggacag caggcacgat gtcgctcgtg
     4741 cgtttaagca gcacgcctgc gccgcgcttc gtcaacatgg tcgccgcgcc cgcctcttgc
     4801 ggcatcggtt cggtcgtcgc gtcggcaata atcggcacgc ggcaagcgag ggcttcgaag
     4861 gtcgtcaaac cgccgagttt cgagatcatc acgttggcgg cctgcatcag ttgttcgacg
     4921 tgttcggaat agccgatcac tttcacgggg aacttcgcac gcgctgcgac cagttcggct
     4981 tcggcgcgca actctttgtt gttgcccgcg agaaaaatgg cctgcacgtc gagatcgccg
     5041 cgcacgagtt cgcggaaaat ttccgggatg ttgccgccgc cgacccagcc cgcgttgacg
     5101 aagactgtga acttctgcgg gtcgagtccg agcgcgttgc gcgcggcctg cgcggcgagt
     5161 tcgtccggca aattgaactt cggatgcacg ggcatccccg aaatcttgat ccgctccggc
     5221 gcgacgccgt aatcgatcaa ctgctgccgc gcctcttcgc tcgccacgag atagagccgc
     5281 acatcgtcgc aagcccaacc gcgccagaag ccgtagcacg ggtcggtcac aacggtgacg
     5341 agcggaatct tatcggcgag tttcaattcc ctgagcacgc gcccgatggc gtgctgcgtc
     5401 aaaggatgca cgctgacgac gacgtgcgga caccaacgct cgaacaactc gtgcacgtag
     5461 ccgacgctgc gacgatagaa aaactcgcgc gtctccgggc gaaaacggtt caccgcccag
     5521 tagagatatt tcatccagtg ctgtcggtga cgcagcaccc agttataaac gttaacgagt
     5581 ttcgcagaga tcgaatgcga ctcttcaatc gcgcgcacga cgcgcaccgc gtaagagttt
     5641 cccttgaaaa acttctgcaa gcccgccgca atcgccacag ccgccgagcg atgcccgccg
     5701 cccgtgtcgg acgagatgat cagaattttc ggaactcttg cacgaaacat aattttgagt
     5761 ttaagggttt gagttcagag gttagttaag aatcagttca gtccttcact gaatctctaa
     5821 cctctgatct ccagcccttg ctttcaggct ttaactgtca aaccttccgc acgcgcttcg
     5881 tcgcgctcga tctgtttgcg cgacttcttc atctcgcgtt cgagacgctt gatctggatc
     5941 aagtgtttcg ggttgaacgg gagcgacgga tagtaactgt tcgattcgtg cgtgcagaag
     6001 cagccggact tcgctttccg gcgtcgctcc tgcatggcgg gcgagagcca gagtttctgg
     6061 aagttccaat cgtagtcgcg caagtttccg acggcgtcga gattctcgca ggaggagact
     6121 gtgccttcgt cgtagatgac gccgcccgcc gtgcccgcgt agcaggtcat ctgcgcctgc
     6181 tgctcttcct gcgccttggc gatgaggccg tgcatgtaga tgtctatggc ggctttgaag
     6241 aaacctgcgt cgccgccgta gttgttcttg atggctgact ggcgcgaatc ggaatcgatc
     6301 atgtgcgcga gttcggcgta gcgcgcgtga tcgatgtcga gttccttcgg gtcggctgaa
     6361 ggcggacgaa tgtagttgaa gttgaccttg tctggcttca ggtcgtactt gagaaaatcg
     6421 taccactcga agatggtgtc ctgattcgtg ttcatgaaac aggtgcacgt ctggatgtcc
     6481 agacgcgggt actcgcgctt gatcttctgg agttcgcgcg ccgtgtcaat ggcgatgtcc
     6541 caggagccgg gcttctggcg aatcttctcg tgctgatcct ttagaccgtc gatgccgagc
     6601 gcgacggcga cgttcaggtt cgggcactct tcgagtatgc gcgtcacgtc gggcagaatg
     6661 cgcttctgaa tctgcccgtt tgacatgaga tagaccgatt cgagtttgtt gttttcgtag
     6721 aaggcgcgca cgatctccgg caaatctttg cgcgtgaacg gctcgccgcc cgccagaatc
     6781 aagacgccga gattgccgcc caaagtctcc gagacgcgct ggatttcgtg cgttttcatc
     6841 tggagttttt tgcgcgggcg gtcgttgagt tcgtccgtaa agaaacagtg cgtgcagcgc
     6901 atgtcgcaga cggaggtgac gagaatgttc aggagcacgg gcgcaaacgg ctgtccgagc
     6961 gcgagtttcg cgtaacgttt aagaagttct ttggtcgtgt aatccatttt atgccttcaa
     7021 aaaaaaacgc gctttcaact cgcgcggatg acgcgtcgcc cgaaccgcga agcgacagaa
     7081 gaatgtaacc gcctgcgcag caaaaggcaa actttccagt ccgcacgagc cgcgcctcgt
     7141 atctttgatg ctaatttccg ttctcacgct cgccgcgaac tttgacgccg agggcgcgca
     7201 tccgccggta gagatggctg cgatccatgc ccatcagttc ggcggcgcgc gtgacgttgc
     7261 cttcggcttc ggcgagtttg cgccggataa actcgcgctg gtgtgcctcg ctcgcctcct
     7321 tgaaggagtt gaagcgatag acggcgacgg gcggggcttc gcctccgagc ggcggcagat
     7381 cgctcgcgtt gaccttcggc ttgcccgtca tgatcacgac gcgctcgacc gtgtttcgca
     7441 gttcgcggac gtttcccggc cacgtgtagc tttgcaaagc gtccatcgct tcgagttcga
     7501 aacgcttggc gggcttgccg taggcggtgg aaaatttccg gttgaaatgc tcgaccagca
     7561 gcggcacatc ttcgagccgg tcgcgcaacg gcggcacttc aaacggaatc acgttgagac
     7621 gatagaagag gtcggcgcga aaattcccgc gctcgatctc gtcttccaga cgcttgttcg
     7681 tggccgccac cacgcgcacg tccacgcgaa tttgcgcgcc gctcccgacg ggcgcgaaac
     7741 gctgctcttc cagcacgcgc aaaactttcg cctgcgtgcg cgcgctcatg tcgcccactt
     7801 cgtccagaaa gagcgtcgcg ccgtcggcca attcaaactt gccgcgcttg gcggcggtcg
     7861 cccccgtgaa cgcgcctttg acgtggccga acagttccga ctcgatcaac tcttcgggaa
     7921 tggccgcgca gttgagttcg atgaaggccg cgcccgcgcg ccgcgactgc gcgtagatgg
     7981 cacgcgcgac caactccttg cccgtccccg attcgccgta gacgagcacg cgcccgtccg
     8041 tcggcgcgac cgccgcgatc tgttttcgca gggcgcgcat cgccacagac tcgccgacca
     8101 tcgcgtgctc ttcgttcatc tccgccgcga gcgtcgcgtt gaggcgttcg agcgcgcgct
     8161 ggcgcagggc gtttcgcaca gtcagaatcg tgcgctcgat ggagagcggt ttttcgatga
     8221 agtcgaacgc gccgagtttc gtcgcgcgca cggcggtttc gatgttgccg tggccggaga
     8281 tcatcacgac cgccgagtct gcgcccgtcg ctgcgaggcg tgagagagtc tccagaccgt
     8341 ctatgcccgg cagccacacg tcgagcagca cgcaactata agcgcgccgt tcgatggctt
     8401 gcaggcactc ttcgcccgac gctacggcat cgactgtgaa gccttcgtct tcgagcacgc
     8461 cgccgagcgt ctcgcggatg ccgcgctcgt cgtcaacaat gagaatggaa tcagacatta
     8521 gctcaacgat gaaggatgaa cgatgaagga cgaactggaa accgattcat cgttcatcgt
     8581 tccgcgttca tcgttaggtt tatgcgtttc atccgtgacg gggatttcga cgagaaactt
     8641 cgcgccgcgc ggtcgattgg gttcggcgcg gatgcgcccg ccgtgttcgt tgatgatgcg
     8701 ttggacgatg gcgaggccga gtccggtgcc gcgcccgcgc gtggagaaat aaggttgaaa
     8761 gagacgcggg aagtcgggtc gctcgatgcc gtggccgctg tcgccgatct ctatgaggag
     8821 catggcgcgc gccgggtcgt gtccggtggc gacggtgata cgccgctcgc ccgtcgcgtc
     8881 ggtcatcgct tcgagcgcgt tgtcgatcag gttgacgatg gcgcggcgca tctgttcggc
     8941 gtcgagcagg ccgggcggca gcgtgcgcgc cagatgcacg tcgaggcgca cgtcctccag
     9001 acgatcttcg tagagcgcga ccgcctgacg caccacctcg ttcaagtccg ccgcttcaag
     9061 ccgcgcgtgc ggcaggcgcg caaagcgcga gaactcgtcc accatcgctt tcagcccggc
     9121 cacctcgcgc gtgatcgtcg cagtgcattc ttccaccact tgcgccacgc gcgcctgctc
     9181 tacgagttcg ggttcgacag tgcggttcgc gtcgccgctt gacgaaaccg cctcgccgtt
     9241 cgacgcatct tcgtgcgccg gttggctgcc gtttcgcatg cggcggaagt tgcgcgcgat
     9301 gcgttcggct gagagttgaa tgggcgtcag aggatttttg atctcgtgcg ccatgcgccg
     9361 cgcgacttcg ctccacgcgg cggcgcgctg tgcggccaga agttcggtca ggtcttcgat
     9421 gacgagcacg atgccgcgtg aggagcgcgc ccgtgcgccg ccctctgtga agttgccgag
     9481 cgcggtggcc gtgagcgcga cgggcacgac gccgccgtcg attgatgcgc cgcgcgccag
     9541 ttccatctgc cacgccgcgc gccccgtgcg ccgcgcacgc gtcagcacgc gttccagcac
     9601 gagatgatct tccatgctca acatctcttt gagcggcgcg cccgctgcag gagattcgcc
     9661 aagccggagc atagccacgg cggcggcgtt gatggtggtg acgcggttct cttcgtccag
     9721 agagatcacg ccggtcgaga gcgattcgag caccgtctcg atgtagttgc gtcgctcgtc
     9781 gagggcgaga tttttttcgc gcagttcgcc cgctcccgct tcgatgcggc ggcgattctc
     9841 ttcgagttgc gcggtcatct ggttgaagga ggcggcgagg atggcgagtt cgtcgtcggc
     9901 aatggccgtc acgcgatgcg cgagattgcc gcgcgccact tcgtccgccg cttctgccag
     9961 cgcgcgaatc ggtgcgccga tgccgcgcgc cagatgaatc gcgctccacg tcgcggcaaa
    10021 cagcaggagc agcgtcatca agccaatcgt ggacagaccg agcgagcgca ccttgcgctg
    10081 ctcccggcgc aactgctcga acgtctccga accggcgacg atgcgctcca aactcgtctt
    10141 aacgcggctc gcacgcgccg ccaccagcgc gcggccatca gccagcgaga ctttcgccac
    10201 tgtgaactta ttactgaacg cgccgctccc gccctcagcc gcgcgcacgc gactcaactc
    10261 cggttccaac tctccggcgc gcgccgcgaa ctgtggcgcg attgtctgcg cgataaccga
    10321 cccgtccgct gcaatcaact gcaacgcggc gaactgcccg ccgtcgctcc actgcgcaag
    10381 gctcgcctgt gcgacgttcg acgctgcgct ctccgtcgtc gccgcagtcg ccgggttctg
    10441 agtcgtggcg gcgttacccg gcgcaacgcc ctgtgtagct ccggcagctt gcgtgttggc
    10501 ggtttgcggc gtcgctacat tttgaatcgc gtctcgctca agttgctttg cgacgagcga
    10561 ggtctgttca agcagatgcc gcacctgctc gtccatgtct tcgcgttgcg cgtcgcgcgc
    10621 cacgcgctgc acgtcttcgg gcaggcgtcc gaaccatttt tcgagcgagc ggttgagaaa
    10681 gaaataggag aagagcgcca tcacagtgat cggcaggagg ctcaccgtga tgaaatagac
    10741 gacgagccgc gtcttgatct tcgagccgag ttcgtgcgcg cgccgctccc ggcgcagttt
    10801 caaaaggttg cgcacgaaga tgaaggcgaa gacgatgaag gcgacgaagt tgagcgacga
    10861 gagcgcgtag agcagcagcg tgtcggcggc ggtgtcgggc gtgaagactt cccacagccc
    10921 gaagacctgc aaggcgacga gcacaaagag cagagacagc acgagactgc ccagcagcca
    10981 cggcgcgctg cgtcgtttcg gcttcggctc gttcaactct tcgggcgtcg gtgcgtctgg
    11041 gttgctcatg ttctggcttc gcggatttga agtttaacac gcgcccgcgt gccggagcat
    11101 gttttgcgcc tcaacattcg gatcggcttt cagttacaat cggcggcgac ttccgagact
    11161 tccgaagcat atccggccac agacgctttc acacgatgac gcctctcgac atttttctca
    11221 atcgccacgg gcgactgcgc agcggttggc gactcgcaat cttctgcgcc gcctactacg
    11281 ccgcgctcgc agctatcttc accaccatct acgcggtatt ttttctcctc aaaatcccca
    11341 acgtggacgc gcggctcgcg ggaacgctcg gctacatcct gcaatccttc atacttttat
    11401 cgaccgctct cgtcgtcggc tgggcttgcg ggcggctctt tgaagacttg ccgctgcgcg
    11461 cgctcggctg ggcgcgacat cgaggctggc tgcgcgacct cctcttgggg acgcttctcg
    11521 gcatcgcgtc gctcatgctc gccgcgctgc tcgcgaccgt gtttcgcggc tttcagttca
    11581 gcgtcacgcc gacaatttca agtgtcgcgt tcctgaagac gttcttcggc tccgccgtca
    11641 tcttcatctt cgcggcggcg ggggaagaga tgttgtttcg cggttatccc ttgcagacga
    11701 tgatgcgcgc tctgcccttc atcgtcgcca tcctgccctc gtccgtgctc ttcgcctacg
    11761 tgcatctcaa taatccgaac gtctcctggg gcttcacctt catcaacacg acgctcgcgg
    11821 gcttctggct cgccgtcgct tacctgcgca cgcgtagcct ctggttcccg ctcggcctcc
    11881 actgggcttg gaactgggcg atgggcgcgc tgctcggcct gcccgtcagc ggcatcacga
    11941 gtctgacccg cgccccgctc tttcgcgcca cggacacagg ccccgcatgg ctcaccggcg
    12001 gcgcatacgg catcgagggc ggcgcggcct gcaccgtcgc ccttatcatc tcgacgatct
    12061 ttatctggaa gacgggactg ctgaaagccg acgaagagtt gaagaagttt acggacggag
    12121 agcagaccaa aaaagtgatg agtgatgagt gatgagtaat gagttaaaga caaagcatct
    12181 gttttaactc attactcatt acttattact catcactcat cactttatcc atcactcatc
    12241 acttttttaa cgttcgttaa tcggcacgta cttcaagtcg cgttggccga tgtattcggc
    12301 gcgggggcgg atgagtttgt tgttcgcgta ttgttcgagg acgtggcctg tccaaccgct
    12361 gatgcgcgag acggcgaaga tgggcgtgta gagttcgagc ggaatgccca tcatgtaata
    12421 ggttgaagcc gagtagaagt ccacgttcgg gtacatgccc ttctcgcggt gaatgtactc
    12481 ctcgatcttt ctggaaatct cgtaccactt cgtctcgccc ttcgactcgc cgacctcctg
    12541 cgagaacttg cgcaggtgcg tggcgcgcgg gtcttccgtc ttatagacgg cgtggccgat
    12601 gcccatgatc ttctgcttcc gcgcgagcgc gtcgcggacg aaatcctccg ccttatcaac
    12661 ttcgccgatc tgcaaaagca tcttcatcac gttcgtgttc gcgccgccgt gcagggggcc
    12721 ggagagcgcg gcgatggccg ccgtcaccgc gccgtacacg tctgcaagcg tgccggcgac
    12781 gacgcgcgcc gtgaacgtcg aagcgttgag ttcatggtcg gcgtgcagga tcaaacagat
    12841 gtcgaagatg cgtgcttcgc gctcggtcgg catctcgccc ttgagcatgt agagaaaatt
    12901 ggtggcgatg tcgagttcgg ggttggcagg gatcggctct tgcccctgac gcaggcgttc
    12961 gatggcggcg acgagcacgg gaaactgcgc cgtgagccgc gtcgcagtgc gcagcgcgcc
    13021 ctcgcgcgag atgtcgcagc attccttatc gtagaagtcg agcgcggaga cggcggtacg
    13081 cagcatgtcc atcggctcgg cttcgcgcgg gaactgccgt atcaactcga tgacctgcgg
    13141 gggcaactcg tagtgctcgc cgatggcgcg cttgaactca tcgaactccg tctgcgtcgg
    13201 caggcgacca tgccagagca ggtagaccgt ctcttcgaag ttcgaatgct cggccagatc
    13261 gtgtatgtcg taaccctgat agataagtat gccctgctcg ccgttcacgt cgccgattga
    13321 cgaactggcg gccacgacgc cgcgcaagcc cgccccgccc cctgcggcgg cagcagcagg
    13381 agcagcggcg gcaggcgtgc tcgcttcctt cggtgtctcg gtggcaggcg cggtggcggc
    13441 ttccttcgcg ttttccatga tgggtatcct tgattttgat taagttgagt cgaagataac
    13501 aaatggggtg acaagtgaca agtgacgggt ggcgtgtgaa aagaggtgaa tcgtaaatcg
    13561 taaatcgtaa acagaaacga cccgcccgct tgcctctcgc cgaccggcga cgcgcaggat
    13621 cttcttataa tttccgaccc gagacgcgca cgagttggcc gcgcgaggca ttgagaagtt
    13681 gcgccgcaga tgcacggtca gccgcgatct caagcaatcc cgcgctgccc cagatggcaa
    13741 acagttcgcc cggcggcgcg cccgccgctt ccgcgtaaaa tctgcgaaac gaactgatct
    13801 cgcgcccgcc aatctccagc ttccggcctt ccgcgagttg ctcatccgtc agctcgcgcc
    13861 gcgtgatgtt cgtgacgaga ttgccgaagc gatctatgtg aatgatccgc gccgaccacg
    13921 cgccgtcccc ttcgcgcaga ggcgcgagcg gggcgagccg tcgcatctct ccatcaatca
    13981 attcgcccaa cgcggagggc ggcacgttcc gcgagagcgc gcccgcaacc ggggcgaaga
    14041 tgtcgcgccc atgaaaggtt gcgctcactt gcgtgcgaaa gaagttttca ttcgtcagat
    14101 gatagacgcg tgcgtccgtt gcgccctcgc agacataact gaagaggccg ttgtcgggtc
    14161 cgacgaagaa gtgcccgtgt gctgcgatga ggagcgggcg acgcgcggag ccgacgccgg
    14221 gatcgacgac cgcgacgtgg atcgtctgcg gcggaaaact gccgtaagcg gcgagcagcg
    14281 tgaacgcgcc cgcctctatg tcatgcgcgg gaatctcgtg tgtgatgtca acgatgtgcg
    14341 cttgcggatc gagcgagaga atcacgccct tcatcgcgcc gacgaaataa tcggcagtgc
    14401 cgaaatctgt gagcagcgtg atgattgaca tgggagagga agtaagggga aaggtggaaa
    14461 ggggaaagga gaagacgggc aacgcggcgc gtgcttcttt tcccttcccc tttagccctt
    14521 agcctttcac cctttcggct tattggattc gcgtctcggt ggaggtgcgg atgagaagtt
    14581 cctgaccgcg ttggaggcgg acatctttgc cgcgcgaggt gatcgcgccg cccgcgccga
    14641 tgctgccgcc gatgaccgcg ccgatggccg cgcctttgcc gccgcccgca atcgcgccga
    14701 tgatcgcgcc gatccctgcg cccgcgccca ctttcgtcac gtcgtccttc gtcgtgctct
    14761 ggccgcgcac gccgccctct tcgtcaactt cgccgacgtt gccggttgac tgtccgacca
    14821 cttcgatgac ctgcgcgttg aggttcgtga agcgaccgtc gggcagactg atctgttcga
    14881 agtcgagttg caactcgccg gagccgcgca ctttgccggg acgcttgacg cccttgacgc
    14941 gcccgacgat gcgtgcgccc tcgtactgtg cgggttcgat gacgcgcgct tcgaaacggt
    15001 cgccgcgctg cgtcgcatcg gtcgagaggt tcgtcagcag ttcgatgcgc atggtggtgt
    15061 ccatgggcag atcgagcgaa ccgatctggc gcaccgcgcc gccgctggtg ctgtttgaat
    15121 cgctggcgcg cgggtcgggc atcgagtcgg aagtgccgcc cactttgtcg ttcacgccgc
    15181 gccccacgtc gttagagggc gctgcgccgc gccgcgagag tccgggcggc acgacggaat
    15241 cgaagccccg ccggtcgaat cccgctgtgt agccgctctc gaaaccttgc tggtaaccgt
    15301 cgcggtaatc ttcgcgtgcg ccgtaggcgg ggatgtaggc gcggtctgcg ctcacgtaat
    15361 cttctttgtt gcgatagtcg cgcgcggcgc gttcgattga atcgcgataa cccgcctggt
    15421 agccgtcgga atagcccgtg cgataaccgc gttcgagcgc gccgacctgc tgcacggcga
    15481 cgacgggcgc gtccgtgttg tcgcttgcag cggcgcgtga cataaaggtc gtggggagca
    15541 gggcgactgc cagcgcgaag gatacggagg cgggcaaaag gcgtttgcga atctgcatat
    15601 tttctctcct taaaaattgt tccggcaaaa gggacgcgtc tcggatagtc tgcgcgcgca
    15661 gcaaacgcat ctcacaacag gtttgaagtt ttctctgaag ttctccgcca ttcggtctat
    15721 gctaacactt caaagaggcc gccgtgaacc ggctttttga agccgcttga ccgcacccac
    15781 ctgcgccgct agactcggaa ccgtctcgaa gcagactacg aagcgagccg ctcgttcaac
    15841 ttgcgccagc cgtccggcgg gagagaattt gccgaccggc gcgcagggtt gtcaattgct
    15901 tgtgatgttt gatcgttaac cgtcagttgt ctgttttatt tgcccgtcgc cagccgcccg
    15961 ctctcagttg tcagtttgtg atctatgcgc cgatggctaa aggttcaaac tgacgtgtga
    16021 cgacggacaa agaacaattt tatgcgcaga gtttatttag acaatagtgc gacgacgccg
    16081 attgacccgc gcgtggtgga ggcgatgttg ccgtatctga cggagaagtt cggcaacgct
    16141 tcgagcgtgc acttttacgg gcaggaggcg cgcgtcgcgc tcgaccgcgc gcggcgtgaa
    16201 gtggcggctc tgattaacgc gcgcccgaac gagttggtct ttctctccgg cggcacggag
    16261 gccaacaacc tcgccattcg cggcatcgcc gagacagccg ccgaacacgg acggcacatc
    16321 atcacttcgc agatcgaaca ttccgccgtg cgcggcgtct gcgatgcgct ggagcggcgc
    16381 ggctgggaag tgacgcgcct gcccgtctat gcagacggca tcgtgcggct cgaagacgtg
    16441 cgcgccgcgc tccggccaga cacagtgctc atcaccctga tgctcgccaa taacgagatc
    16501 ggcacgattc aacccgtcgg cgaagtcggc gcgctcgtcc gtgaggaacg cgaacagcgc
    16561 gggcgcaaat atctctggct gcacacggac gccgtgcagg cggcggggcg cacgacggtt
    16621 gacgtggacg cgctcggctg cgacctgctc tcgctctccg cgcacaagct caacgcaccg
    16681 aaaggcgtgg gcgcgctcta cgtgcggcgc agcgtccggc tcacgccgca gtcaatcggc
    16741 ggccatcagg aacgcgaacg gcgcgcgggc actgagtcag tcccgctcat cgtcgccttc
    16801 ggcaaagccg ccgcgctcgc acgcgaagaa ctcgacagcc ggatcgcaca cctgagtcgt
    16861 ttgcgagatc aatttgagcg cggcgtcgag gcgtcggtcg cagagatcgt cttcaacggc
    16921 gacagggcgc ggcggctgcc gcatctctca aacatctcct tccgcttcat cgaaggcgaa
    16981 ggcttgctca tcaatctcga catgcagggc gtcgccgtct ccacggggtc ggcctgctcc
    17041 tccggctcgc tcgaaccctc ccccgtgatc cgcgccatcg ggcgcgacga cgaactcgcg
    17101 cgcggctcga tccgtttcag cctcggcaaa gagacgagcg aagaagagat cgcctacgtg
    17161 ctcgaagtgc tgccccgcgc cgtcgaaaac ctgcgccgcc tttcgccgct ctaccagcgc
    17221 gcacttgcca cgcgcgcctg cgaaacgacg aacgcctgag ccgcaagttg caggctctaa
    17281 gttcaagctc ggagtttgaa actctgagtc tttaacttga aacccgcgac ttgaaatttg
    17341 ttccctgatg ccctactccg aggctttcaa agatcatctc gcacacccgc gcaacggcgg
    17401 cgaactcgcc gacgccgacg cctccgccga actaacgaat cccgcctgcg gcgaccgcct
    17461 ccgtctcacg ctgcgcatcc gcgacgggcg catcagtgcc gcgcgcttcc tcgcctacgg
    17521 ctgcccgccc acgctcgcct gcggctccgc gctcaccgag atgctcgaag ccctcaccat
    17581 cgcagaggcc gcgggcatca cccgccaaca actcacacac gcgctcggcg gccttcccgc
    17641 gcgcaaaagc cacgccgccg cactcgccat cgagacgttg cacgcagcat tgaacgaccg
    17701 ccgattgtta acggaatcga gcgagtgaag ccacacgcat tgtctgtcgt agccgactgc
    17761 cgcaagtgat atactgcgcc cgtctagcct ttgagtcgtt cgcgaggtaa ctatgtcccg
    17821 ccaaccccag acttacatca cgcccggcga atatctggcc ttagaacgcc agaccgaata
    17881 taagcacgaa tatttcgacg gcgaaatttt tgcgatggtc ggagccagca gaaagcagaa
    17941 tttgatcacg gtcaacatta cccgcgagtt gtcgcagcag ttaaagggga aaccttacga
    18001 agtctatagc agcgatatgc ggatcaggat tccgaacgcc aacatttaca cctacgcgga
    18061 tttgacggtg gcgtgcggcg agccgaaatt tgaagacgcg caggttgaca cgctgcttaa
    18121 tcctgttttg atcgtcgaag tcctctccaa atcaaccgcc tcttatgacc gcaggcaaaa
    18181 gtttgccttc taccgcacca tcgaatccct gtccgattac atactcgtct cacaggagag
    18241 tttccacgtc gagcaataca cgaaacaaac tgacaatcgc tggctgctct cggatttccg
    18301 ctcgatggaa gatgcgttgc agctagactc tgttcaatgc tctctcgccc tcagagaaat
    18361 ttacgacaaa gtttccctct cataatctct tcgcacccga aacgaaacag gcgacgcgat
    18421 ctagatcgcg tcgcctgtcg cctgtcaggg ggaaactctc ttactgctgc cggactttcc
    18481 ccggcgtcat catgcgcacg cgacgcgggt gtgattcttc ggccttcaaa gtgtagtcgg
    18541 agggcacttc gaagagcgtg cgcgccggtt cgctgcggtc gatgttggtc agccggtagt
    18601 tggtttcgcc gaagcgcggg tcgctgtgtt tggtcatcac gacggtttgc agttcgggcg
    18661 aataccaacg ctcggagacg acgttgatcg ggcgttcgtt gccgaacgtg cccgccggga
    18721 acgtgacggt ggtacgcgtg ccttcggctt ccacgccttc gatgttctgc ttgccgagcg
    18781 attccgttac cggcttcggc accgccacgc gcacctggag gccgtcgccc tccggcgcgt
    18841 gtcccgttgc gggcgtgcgc aggaagctaa cctcggaggt gggtgcatct tcacccttcg
    18901 ggagcgcgaa gacgaaagag atcatgcccg acactttcac cgcgttgccg gagagtttgg
    18961 tcggggtaaa acgccactct ttcgcggcgt ccacggcagc ctgctgcaag agcgggtggc
    19021 cggagttggc cttcgcggat tcgacttcgc ccgcttcgtt gatgacgatc tgcacggtca
    19081 cagaaccctg cgcccccgcc gccttcgcaa tcgccggata cgtgggctga actttcttga
    19141 tggccttgcc cgacaaaccg gggtcggcag ccttcaacgg agcggaggag acaaccacgt
    19201 ctgcgctccc gccgctgcgc accaagacct gcgtgcgcgc ttcgccgggc gcgggatgga
    19261 gttcgttaac ctcttccctg gtgcgcgtga agaagaacat gttgggcatc ttctgcgcgg
    19321 tgcgtgtgcg cgggttgaga acgtagtgtg cgcccgatac agggtcgttg atgaagatgg
    19381 tttgcggcgg ctcgtcggcg gtcgcccagg gaccgatgtg attgagcgtc tggtcgcggc
    19441 gcgtgcgtcc ttcactgtcg cggtagaggc tggccttcga ggtgcgcgtg atgcggttgc
    19501 cgtcggccaa cgtctggacg gattcggtga cggcctccgc agagtaaggc gcgcctttga
    19561 cgatcttgct gtcgaagtgc atctccgacg acataaaact gaagttgaaa gagttgttcg
    19621 gtgcgcccgg cgcgatgtgc atctcgaatg gctctccgga cggctggtgc ggcacgagca
    19681 ccgcttcgcg cccgacgatc acgtcgtgct ccggcgtcgg cggcggggct tgtcgcgttc
    19741 tctgttgagt ttgcgcctgt agcaacgcgc ccgcgccgtt ggtcagtaaa gcaagcgtgc
    19801 aaaatagggc tgtcagccag cgtctctgta acatcatttt catcatcctt atcctttcca
    19861 gattatcgcc ggtttgttcc gggcgtgcgt cgctccgacg gcgacgcatc tctcaatcct
    19921 tcaacttcaa actacaggct cgcgcgctca gcgctcaaaa cggatggcgc gtgccagccc
    19981 gtcgccgccc agaagcacgt cggccttgac cggctcgttg ccgcgctctg cattcagagg
    20041 caatccgagc gacgcgagcg cggcgcgcgg cacctgcact cgcaccaact gcccgctctc
    20101 aagcggcggc gcggcgggcg cgtcggcgac gagtgaaata aagtccgtca cgctctccgc
    20161 ttcgccgggt ttcgccgcgc cgttcaaggc catctctcct tcgacgcccg gctcgataat
    20221 cgcgtggccg ccgtcaatca cgtgctgcgc tttgatcatg gtcggcacgc gtcgttgacg
    20281 gctcgcaacc ttgacgttcg tgcgcggaga ttggacgcct ttaaacgcgt ccggcatcgc
    20341 gcgctccggc gtcggcttcg cttcaattga aggcgcgctt gaaggcgcag gcgacggcga
    20401 agcggcaagc gttaactctc tgtccggcgg tgacgcttta ccggcaaccg tctgccggtt
    20461 cgtcgccagc ggttgcggcg tgggcggcgc ggtctgtgtg aagcgtagat atagcgtggc
    20521 gaggacgacg agcacgagag aggctgcgat ggcggctgtc gcgttaaaga cggagcggcg
    20581 cgaaaagcga ctcgcgtcgg gcgtagttct cgcatcgcgc gtaagctgcg agacgggcgc
    20641 aggcgtggag gcgacggtcg ctgtcgccgg tgcgtgtcgt tgcgggaggc tttcatggaa
    20701 agcagccaac agtttcgctt cgacgcgtgc gggcgcgctc aaatcttcta tgtcttgcgc
    20761 gacggcgcgc agcgcacccg tcaaactctg ctcctgcgcc agacgcgcgg cgcacgcctg
    20821 acaactttca gcgtggcgca ctgcgccgtc gcgcgatgcg gcatccatca tctgcccgcg
    20881 cgccacttca acgatgaacg atgtgaattg ctgacagttc atgcgaaaca ccttagcgga
    20941 tcaaaatctt cggcgacgcc cgcctcccgt cgctgcacct cgcgcaaacg ctcgcagagc
    21001 agagcgcgtg cgcggtgcag ccgcgaacgc accgtgccga ccgcgcaatt caacgcggca
    21061 gccgcctccg cataactcat ctcgtgcagt tcgcacaaca cgaccacttc acgataatgc
    21121 gcgggcagag ccaacacggc ctgctgcacg gcttcgatca actcgtggcg cgtgagttcg
    21181 gcgagcgggt cgtgcccggt cgtcgccgga tgctgcgtcg ccgtcgccgc gcccccgtcc
    21241 tccgccgtct cttcgacgag tgccacgaaa tgcctctcct tctccaaacg ccgcaagacc
    21301 tgattgcgcg cgatgccgta gagatacgcg gcgagcgtgc cgcgcgacgc atcgaaaatc
    21361 tgcgcttcgc ggcgaacgag cgtcataaaa acttcctgcg tgacatcctc cgcgatcccc
    21421 tcggagccgc acatttgcag cgcgaaacga tagatgcccg cctgccgcct gcgatagagc
    21481 gcggtgaaag cgtccgcatc tccggccaga attagccgca acagttcttc atcgctgtgc
    21541 tcaggagagg ctttcatctt caatcgcggg acgctctatc cttgctcgtc acacgtaatt
    21601 cgcccggcgc ggcgaaaaag ttccacaaag tcggacgggg cgattgaata cttcggggcg
    21661 gctcgaatgt gggcggggcg cgtcgccgtt ctttaacacg cgatgcgttc gcggaagttc
    21721 ttcagcgaga ctgaaaactc gtgtgcagtt gcaactttcc cctgctcagc gcggagttgg
    21781 cgagtttgag ttggacaact gagggcttcc ccgtgtttgt cgtgttgccg ggcgtgtagc
    21841 cgagcacgta ttcgggcatt tgcagttcgc ggttgagagc ggcggcggtt gtggcaaact
    21901 ccgacgtttt tttggggaag acggcgcggc ctcccgtctc gcgcgcgagc gtttcgagca
    21961 actcgcgcga cttcctgcgc ccacccttca cctgacggaa agcgtcgtcg ctcaacgcgc
    22021 ccgtgaggcc gaagatgaac acatggacgt gttgacgacg cagcaaagtt aacaactcgt
    22081 cgagctttga acgcgggtcg cgctcgccgc cgtccgtgat aacgacgagc gcgcgatggc
    22141 gcgactcgcc gggcgtgcgc gtcgtcaggg tttccgccgt aacgtagagg gcttcgagca
    22201 gagccgtctc gccgccctcc aaatacatct cgtcgagcgc ggcggcgagc atgtttttac
    22261 tccccgtgaa gttttgcagc aactcgatct tgtcgcggct gacgaaacgc acgattgaca
    22321 tttcatcctg cggcctcatg cccgcgacta tcgccccgcc cgccttgagc acgtcgtcga
    22381 acatcgtgcg cagggagccg ctgttatcaa ccagcagggt gtagctgag
//
LOCUS       DASWNS010000369         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_197032, whole genome shotgun sequence.
ACCESSION   DASWNS010000369 DASWNS010000000
VERSION     DASWNS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197032"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VGB76_13020"
     CDS             complement(<1..547)
                     /locus_tag="VGB76_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099862.1"
                     /translation="MRCSLLALLLKLRARFVPFVAVALAGFQLVLPVETSAQRRRPRR
                     VLQPPSVSRVNASASQQDARLAQIANEYLRGYFAFYPSEATALGVHEFDGLLEARDAA
                     TLARETRRLRGALASLARVPEARLSAEARYDYLVLQSHARAELLELEEVRMWQRNPNL
                     YNRLLASGVDNILKRAYAPISA"
     gene            1266..>1660
                     /locus_tag="VGB76_13025"
     CDS             1266..>1660
                     /locus_tag="VGB76_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099863.1"
                     /translation="MQFRVNLRSATNPPNTPPPAATMEPPATLTATLMFQRPPGSGGG
                     SGTENECPPDDFNGDIYYSTCTPIIVDVLDNGFDLTNAAGGVDFDINSDGVKGRVSWT
                     AGGSDDALLALDGNGDGLITLGAELFGYWS"
BASE COUNT          372 a          497 c          512 g          279 t
ORIGIN      
        1 gcgcggagat gggcgcatag gcgcgcttca aaatgttgtc cacgccggag gcgagcaggc
       61 gattatagag attcggattg cgttgccaca tccgcacctc ttcgagttcg agcaactccg
      121 cgcgcgcgtg cgattgcagc accagataat cgtagcgcgc ctctgccgac agccgcgctt
      181 ccggcacgcg cgcaagcgac gcgagcgcgc cgcgcaaacg ccgcgtctca cgcgccagcg
      241 ttgcggcgtc gcgcgcttcg agcaggccgt cgaactcatg cacgccgagc gcggtagcct
      301 ccgaaggata gaaagcgaag tagccgcgca gatactcgtt cgcgatctgc gccagacgcg
      361 catcctgctg cgatgcagac gcgttcacgc gcgacacaga cggcggctgc aacacacggc
      421 gcgggcgacg cctctgcgcc gaagtttcga cgggcagcac gagttgaaac ccggccagcg
      481 cgacggcgac gaagggcacg aatcgcgcac ggagtttgag cagcaaagcc aagagactgc
      541 acctcataat gttagaaagt gaaacaaacg aagttcgcct atgcgtggag agacacgcgc
      601 gagattatca caaaaccggc gcgggcagag gcaaaagaga gcgaagctga aagcgtgatg
      661 accgggaggg tcgcacacat aacacgcccg aagagagtcc gacgcggcga ccctcccggt
      721 cagcagtagt tcgacgcttc gtgaatttgg gaaaagattg actcgctcgg cagcaaagtc
      781 tataagtgag tccgcatcgt cgcgcctctt tcatcaacct caccgcacgg acgaagggga
      841 tactcacaac catgaccaga cgatctacca ccttacgccg caccttattg cttttctgcg
      901 tttgcgctct tactttcggc ggcttgggcg cggctccgct cgaattaacc ggcgcgagga
      961 accggcaaca gatattccgc cgtgtacatc ttggggcggc gacgatgtct ccaaccacga
     1021 ttcccgccga cggcactgca ttagcaaccc tcactgtatc ggtggcgaca tcgacaggtg
     1081 ttcgtgaagg aaccgtggca gtagttgatt taactgaaga tagtaatctg aacggcgttt
     1141 cttacagtgt atcgggcggc aacagaaaca atggtcggac gtgggatgtc atactcaggg
     1201 gcggaggggt tagtgaaact attaggtata cgataagggg agatgcgacc agccccatcg
     1261 gctcaatgca gttcagagtg aatctcaggt cggcgacgaa tccgccgaac accccgccgc
     1321 cagccgccac tatggagcca ccggccacgc tcacggcgac gctgatgttc cagaggccgc
     1381 ccggcagcgg cggaggcagc gggactgaaa acgagtgccc gccggacgat ttcaacgggg
     1441 atatttatta ctcgacctgc acccccatca tcgtagacgt gctcgacaac ggcttcgacc
     1501 tgacgaacgc ggcaggcggc gtggacttcg acattaacag cgacggcgtc aagggtcgcg
     1561 tctcgtggac ggcgggcggc tcggatgacg ccttgctcgc cctcgacggc aacggcgacg
     1621 ggctgatcac gctcggcgcg gaattgttcg gctactggtc
//
LOCUS       DASWNS010000370         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_351548, whole genome shotgun sequence.
ACCESSION   DASWNS010000370 DASWNS010000000
VERSION     DASWNS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351548"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VGB76_13030"
     CDS             <1..1075
                     /locus_tag="VGB76_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0099864.1"
                     /translation="KETDWQGRIFNRFSSRLSSFSIFIALITLGLALAVPHANAQKRQ
                     RKPMPKAVTAAPVAATKAGTKTPANAATKAKPPVVAARSITLKSEPKAVVWFDEVRRG
                     TTDEAGAFEIGKVSPGRHTLRVRAAGFAERTLSLLPAQRGTILVPLTPTTDEAELAFQ
                     QAETARERATSDEARRESAEFYRRALKLRPRFAAAHTGLARVLLDLNDYDGALDEVEE
                     ARRLRPASAEASVVEGRILRALAQNDEAVAAYERALREARGFQPEAHTGLGIIYEEEG
                     KYEEAAAAFRKAIAQLSDTEPVLYELIGRTYERLEKYREAVAAYEKYLSLAPNGKLAP
                     AIESVIEQLRKQAAEQDAPPPVN"
     gene            complement(1106..>1606)
                     /locus_tag="VGB76_13035"
     CDS             complement(1106..>1606)
                     /locus_tag="VGB76_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099865.1"
                     /translation="RCFPQMFLRVLRFVRLPLVLLVIWTIGRFSLGLLGIPYAPRGNA
                     MLSILGLTIISALIYGAMSKRVGNFNWVGTILIGVTLGLFSQILIFFATLITYAADLD
                     TYFRHWDALNIPEGTTAPMAKALATRAGGFIAGPILGTITALIGRAVFGKLAPGGDEA
                     GAARLS"
BASE COUNT          339 a          530 c          500 g          237 t
ORIGIN      
        1 gaaagagaca gactggcaag gccgcatctt caatcgcttc tcttcccggc tctcctcctt
       61 ctcaatcttt atcgctctga tcacgctcgg cctcgcgctc gccgtcccgc acgcgaacgc
      121 gcagaagagg cagcggaagc cgatgccgaa agccgttacc gccgcgcccg tcgcggcgac
      181 caaagcaggc acgaagactc cggcgaacgc ggcgacgaaa gcgaagccgc ccgtcgtcgc
      241 cgctagatca atcacgttga agagtgaacc gaaggccgtc gtctggtttg atgaagtgag
      301 gcgcggcacg acggacgagg cgggcgcgtt cgagatcggg aaagtttcgc cgggacgcca
      361 caccttgcgc gtgcgcgccg cagggttcgc cgaacgcacg ctctcgctgc tgcctgcgca
      421 acgcggcacg atcctcgtcc cgctcacgcc cacgactgac gaagccgaac tcgcctttca
      481 acaggccgaa accgcgcgcg agcgtgcgac gagcgacgaa gcgcgccgcg agtccgccga
      541 gttttatcgc cgcgcgctca agttgcgtcc gcgtttcgcg gcggcgcaca ccggcctcgc
      601 acgagtcctg ctcgatctga acgattacga tggcgcgctc gatgaagttg aagaggcgcg
      661 gcgtctgcgc ccggcgtctg cggaagcctc cgtggttgaa gggcgtatcc tgcgcgctct
      721 ggcacagaac gacgaagccg tcgcggctta cgaacgcgcc ctccgcgagg cgcgcggctt
      781 tcaacccgaa gcccacaccg gactcggcat catctacgaa gaggaaggca agtatgagga
      841 agcggcggcg gcctttcgca aagccatcgc gcaactctcc gacaccgaac ccgtcctcta
      901 cgagttgatc ggcagaacct acgagcgtct ggagaagtac agagaagccg tcgcggctta
      961 cgagaaatac ctgtcactcg ccccgaacgg caaactcgcc ccggccatcg aatccgtcat
     1021 cgaacaactc cgcaagcagg ccgccgaaca ggacgcgccg ccgcccgtca actagagcta
     1081 actaccaacc gaagcgacgc atcaactagg acaggcgagc cgcgcccgct tcatcgccgc
     1141 ccggcgcgag tttgccgaac accgcgcgcc cgatgagcgc ggtaatggtt cccagaatcg
     1201 gccccgcgat gaatccgcct gcgcgcgtcg ccagagcctt cgccatcggc gcggtcgtcc
     1261 cttcggggat gttcaacgcg tcccaatgac ggaaataggt gtcgaggtct gccgcgtagg
     1321 tgatgagcgt ggcgaagaag atcagtattt gcgagaacag gccgagcgtg acgccgatga
     1381 ggatcgtgcc cacccagttg aagttgccca cgcgcttcga catcgcgccg tagatgagcg
     1441 cgctgatgat cgtcaggccg aggatggaga gcatcgcgtt gccgcgcggc gcgtaaggga
     1501 tacccagcaa tccgagggag aaacggccaa tcgtccagat gacgagtaag accaaaggca
     1561 ggcgcacgaa gcgcaggaca cgtaggaaca tttgggggaa gcacct
//
LOCUS       DASWNS010000371         7019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_273828, whole genome shotgun sequence.
ACCESSION   DASWNS010000371 DASWNS010000000
VERSION     DASWNS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273828"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..517
                     /locus_tag="VGB76_13040"
     CDS             38..517
                     /locus_tag="VGB76_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0099866.1"
                     /translation="MKVSAQEEYGLRCLLQLATLGEGEYMTLTQMAEREGISVANAGK
                     LLWILSKAGLVNSIRGTKGGYRLARPASDIHLDEIIKVLDEDVLASHCNSYTGILDAC
                     VHTGNCGIRPVIVGLHDIVQKAMASITLAQLLGTESNVETTLHQISARARQTTHREA"
     gene            580..2016
                     /gene="sufB"
                     /locus_tag="VGB76_13045"
     CDS             580..2016
                     /gene="sufB"
                     /locus_tag="VGB76_13045"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY0099867.1"
                     /translation="MSTIELLTNKEYKYGFVTDIESDMIPRGLSEDTIRLISAKKNEP
                     EFMLEFRLKAYRHWLKMEEPKHWPNITYPSVDYQDIIYYSAPKQKAVKASMDEVDPEL
                     IRTFEKLGIPLTEQKQLAGVAVDAVFDSVSVATTYKEKLKKHGVIFCSFSEAVLEYPD
                     IIKKYLGTVVPTNDNYFAALNSAVFTDGSFVFIPKGVRCPMELSTYFRINNSESGQFE
                     RTLIIAEENSHVSYLEGCTAPKFDKNQLHAAVVELVALDDAEIKYSTVQNWYAGDEEG
                     RGGIYNFVTKRGACRGRNSKISWTQVETGSAITWKYPSCILQGDNSIGEFYSVALTNH
                     AQQADTGTKMIHIGKNTRSNIVSKGISAGRSNNSYRGLVKVLAGAEGARNYTQCDSLL
                     IGDKCGANTFPYIEVSNNTAKVEHEATTSRISEEQLFYLMQRGISQEDAVSLIINGFC
                     KDVFRELPMEFAVEAQRLLGMKLEGSVG"
     gene            2121..2900
                     /gene="sufC"
                     /locus_tag="VGB76_13050"
     CDS             2121..2900
                     /gene="sufC"
                     /locus_tag="VGB76_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY0099868.1"
                     /translation="MLEIRDLHAKVEGKEILRGVSLSVKRGEVHAIMGPNGSGKSTLA
                     KVLAGHPSYEVTGGEVLFDGKNLLELAPDERARDGVFLAFQYPVEIPGVSNAQFLRLA
                     YNEKRKHLGEEELDPLEFKDLLKERAKIVEMDAGLMSRSVNEGFSGGEKKRNEILQMA
                     VLEPKLAVLDETDSGLDIDALRIVSEGVNKLRNADNAIVLVTHYQRLLNYIVPDFVHV
                     LYKGRIVRSGGKELALALEEKGYDWIKDEAGAASQQSATSA"
     gene            2949..4289
                     /gene="sufD"
                     /locus_tag="VGB76_13055"
     CDS             2949..4289
                     /gene="sufD"
                     /locus_tag="VGB76_13055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY0099869.1"
                     /translation="MMVQATKEKSLYRAAFETMQAGDRRALPAWVLRLRESALERFEQ
                     LNFPTTDVEDWKYTNVAPISKGGFAPVAPDSRSEVSAEMLANFIAPEASSRLVFVNGV
                     HRAELSSVENLPAGVVVANLAEALTGEHEFDMRSHLARPSETENDGFNALNAAFLGTS
                     GALIYLPKGAQVAAPIQLLFIAEDASQPPAIFPRVLVVAGRESAATIIESYASLGESA
                     SFTNAVVEVFVGENARLTHYKVQRENERSFHIATTSAELERSGVYDLTTITLGAQLSR
                     HGLNLRLAHEGAECWADGLYLVGTGQHADTHSLIDHASPHCTSHQLYKGILDGKSRAV
                     FNGKVFVHRDAQKTDAMQSNKNLLLSNEARVDTKPQLEIFADDVKCAHGATVGQLEEE
                     ELFYLISRGLHTDLARNLLTYGFAEELVEKIKIESIKQQLDEAILNRLHARLEV"
     gene            4352..5620
                     /locus_tag="VGB76_13060"
     CDS             4352..5620
                     /locus_tag="VGB76_13060"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY0099870.1"
                     /translation="MSGETGAGGEDAAQTVSAWDVARIREDFPVLRQTVNGKPLVYLD
                     NAASSQVPQVVIDRGSRYLREEHSNIHRGVHYLSQRATSAYEGAREKVRGFINARESR
                     ECIFVRGCTEGINLVMHGYGRKFVTEGDEIIVSAMEHHANIVPWQMLCEEKGAHLRVI
                     PMNDAGELLLDDYEALLNERTKLVAVMHVSNALGTINPIKEIVARAHKQGVPVLVDGA
                     QGAPHLKIDVQDLDADFYIISGHKMFAPTGSGVVYGKAEMLERMNPFLGGGDMIRTVT
                     FEKTTYAGLPNKFEAGTPAIASQIGLGAAIDYLNGIDRAAAHAYEQELLAYASEKLTA
                     IEGVRLVGTAREKVGALSFVIEDVHPHDVGTILDQEGIAIRAGHHCAQPVMKRFNVPA
                     TARASFSFYNTKEEVDTLVHAVQKVIEVFS"
     gene            5622..6110
                     /locus_tag="VGB76_13065"
     CDS             5622..6110
                     /locus_tag="VGB76_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY0099871.1"
                     /translation="MSELSELYQQVILDHNKKPRNFKKLETANRQAEGFNPLCGDQLN
                     VYLQLEDERITDVSFEGSGCAISKASASMMTQSVKGKTREEAETLFQEFHQMVTGALD
                     EEATPNSLGRLTIFAGVREFPARVKCASLSWHTMHAALAGEEIVTTENLGPDITSEQI
                     SA"
     gene            complement(6278..6727)
                     /locus_tag="VGB76_13070"
     CDS             complement(6278..6727)
                     /locus_tag="VGB76_13070"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEY0099872.1"
                     /translation="MQRTGTVRRAGRFLKRRPLEAFLLLRMATWIVLLSLLIKFLPLP
                     RVLSLVAASGRSQKPKNSRITQQRLAQLVDALLGLNVLCFTPTCWKRAPVLHRYLALN
                     GTETRIVFGLRKEKESLLAGHAWLEADGRPLLEASPPQYIQTYSFPA"
BASE COUNT         1629 a         1932 c         2101 g         1357 t
ORIGIN      
        1 cgtaagttga taagttgctg atgtggaagc ggtttatatg aaagtctccg cacaggaaga
       61 gtacgggctg cgttgccttt tgcaactcgc gacgctcggc gagggcgaat acatgacgct
      121 cacgcagatg gccgagcgcg agggcatttc cgtggcaaac gcgggcaagt tgctctggat
      181 tttaagcaaa gccggactcg taaattcgat tcgcggcacg aagggcgggt atcgtctggc
      241 gcgtccggcg tccgatattc acctcgacga gatcatcaag gtgctcgacg aagacgtgct
      301 ggcgagccac tgcaacagct acacgggcat cctcgatgcc tgcgtccaca cgggcaactg
      361 cggcatccgc ccggtaatcg tcggcctgca cgacatcgtg cagaaagcta tggcgagcat
      421 cacgctcgcg cagttgctcg gaaccgagtc gaacgtggag acgaccctgc accagataag
      481 cgcgcgcgcg cgacagacga cgcaccgcga ggcttaagac gaagggcgga aacggcgcat
      541 ggccggatta gaagttcaaa gggtagagaa gagataaaga tgagcacaat cgaactgctg
      601 accaacaaag aatacaagta cggttttgtg accgacatcg aatcggacat gattccgcgc
      661 ggtttgagcg aagacacgat ccgtctcatc tccgcgaaga agaacgagcc ggagttcatg
      721 cttgagtttc gcctcaaagc ctatcgccac tggctgaaga tggaagagcc gaagcactgg
      781 ccgaacatca cctatccgtc cgtagattat caggacatca tctattacag cgcgcccaag
      841 cagaaggccg tcaaggcgag catggatgaa gttgacccgg agttgatccg cacctttgag
      901 aaactcggca tccctctgac cgagcagaaa caactggcgg gagtcgccgt ggatgcggtc
      961 ttcgattcgg tttccgtggc gacgacttac aaagagaaac tcaagaagca cggcgtcatc
     1021 ttctgttcgt tttcggaagc ggtgctcgaa tatcccgaca tcatcaagaa atacctgggc
     1081 acggtcgtcc cgacgaacga caactacttc gcggctctca acagcgcggt cttcaccgac
     1141 ggctctttcg tctttattcc gaagggcgtg cgctgcccga tggagttgtc cacctatttc
     1201 cgcatcaaca attccgagtc cggccagttt gagcgcacgc tgatcatcgc ggaagagaac
     1261 tcgcacgtct cctatctcga aggctgcacc gcgccgaagt tcgacaagaa ccagttgcac
     1321 gcggcggtcg tcgaactcgt cgctttagat gacgcggaga tcaagtattc gacggtgcag
     1381 aactggtacg cgggcgacga agagggcagg ggcggcattt acaatttcgt gacgaagcgc
     1441 ggcgcgtgtc gcgggcgcaa ctcgaagatc tcctggacgc aggttgaaac cggatcggcc
     1501 atcacttgga aatatccttc ctgcatcttg cagggcgaca attccatcgg cgagttctat
     1561 tcggtcgcac tcacgaatca cgcgcagcaa gccgacacgg gcacgaagat gatccacatc
     1621 gggaagaaca cgcgctcgaa catcgtctcg aaaggcatct cggcgggaag gtcgaataac
     1681 agttatcgcg gcctcgtcaa agtgctggcc ggggcggagg gcgcgcgcaa ctatacgcag
     1741 tgtgattcct tgctcatcgg cgacaagtgc ggcgcgaaca cgtttcccta catcgaggtg
     1801 tcgaacaaca cggcgaaggt ggagcacgaa gcgaccactt cacgcatcag cgaagagcag
     1861 ttgttttatc tcatgcagcg cggcatctcg caggaagacg ccgtctcgct catcatcaac
     1921 ggcttctgca aggatgtctt ccgcgaactg ccgatggaat tcgctgtcga agcgcaacgc
     1981 ctgctcggta tgaaactgga aggcagcgtc ggataaaact gtgacgagtg acaggtgaca
     2041 agtgacgaga taaagagaga gcttttgttt tagcttgtca cttgtcactg tctgcttgtc
     2101 acactccgaa ggagtaaata gtgttagaga ttcgtgattt acatgcaaag gtcgagggca
     2161 aggaaatctt gcggggcgtg agcctgtcgg tgaagcgcgg cgaggtgcat gccatcatgg
     2221 ggccgaacgg ctccgggaaa tcgacgctcg ccaaagtgct cgcggggcat ccgtcttatg
     2281 aagtgacggg gggcgaagtc ttgttcgacg gcaaaaacct gctcgaactc gcacccgacg
     2341 agcgcgcacg cgacggcgtc ttcctcgcgt ttcaatatcc ggtggagata ccgggcgtgt
     2401 ccaacgcgca atttttgcgt ctggcctaca acgagaagcg caagcacctc ggcgaagagg
     2461 aactcgaccc gctggagttc aaggacttgc tgaaagagcg cgcgaagatc gtggagatgg
     2521 atgcggggct gatgtcgcgt tcggtcaatg aaggcttctc cggcggcgag aagaagcgca
     2581 acgagatttt gcagatggcc gtgctcgaac cgaaattggc cgtgctcgac gaaaccgatt
     2641 ccggcctcga catagacgcc ctgcgcatcg tctccgaagg cgtcaacaaa ctgcgcaacg
     2701 ccgacaacgc catcgtgctc gtcacgcact atcaacgcct gctcaactac atcgtgcccg
     2761 acttcgtgca cgtgctctac aagggacgca tcgtgcgttc cggcggcaaa gagttggcgc
     2821 tcgcgctcga agagaagggc tacgattgga tcaaggatga agcgggggca gccagccagc
     2881 agtctgccac cagcgcatag acgcgcgtgc gcaaactttt agattgatga gacgaaagct
     2941 cgaagagaat gatggtacaa gcgactaaag agaagagttt atacagggct gcctttgaga
     3001 cgatgcaggc cggagacagg cgtgcgctgc ccgcctgggt gctgcgcctg cgcgagtctg
     3061 cgctggaacg atttgagcaa ctcaacttcc cgacgaccga cgttgaggat tggaagtata
     3121 cgaacgtcgc tcccatctca aagggcggct tcgcgccggt cgcgccggat tcgcgtagcg
     3181 aagtgagcgc ggagatgctg gcgaatttca tcgctcccga agcgtcgagc cgtctggtct
     3241 tcgtcaacgg agtacaccgc gcggagcttt cgtccgtcga aaatttaccg gcgggcgtcg
     3301 tggtcgcaaa tttagcggaa gccttaacgg gcgagcacga attcgacatg cgctcgcatc
     3361 tcgcgcgccc gtccgagacg gaaaacgacg ggtttaacgc gctgaacgca gcattcctcg
     3421 gcacgagcgg cgcgctcatc tacctgccga aaggcgcgca ggtggctgcg ccgattcaac
     3481 tgttgtttat cgcagaggac gcgtcacagc ctccggccat ctttccgcgc gtgctcgtcg
     3541 ttgccgggcg cgaaagcgcg gccaccatca tcgaaagcta cgcatcgctc ggagagtccg
     3601 cttcattcac aaacgccgta gtcgaagttt tcgtgggaga gaacgcgcgc ctgacgcact
     3661 acaaagtgca gcgcgaaaac gagcgttcat ttcacatcgc gacgaccagt gcggaactcg
     3721 aaagaagcgg cgtctatgat ctgacgacga tcactctggg cgcgcagctt tcgcggcacg
     3781 gactcaacct ccggctcgcg catgaaggcg cggaatgttg ggcggacgga ctctatctcg
     3841 tcggcacagg ccagcacgcc gacacgcatt ccctgatcga tcacgccagc ccgcactgca
     3901 ccagccatca actttacaaa ggcatcctcg acggtaaatc gcgcgccgtc tttaacggca
     3961 aagtctttgt gcaccgcgat gcgcagaaga cagacgccat gcagtcgaac aagaacctgc
     4021 tgctctccaa cgaagcgcgc gtggatacca agccgcagtt ggagattttc gccgacgacg
     4081 tgaagtgcgc gcacggagca actgtcgggc agcttgaaga agaggaactt ttttatctca
     4141 tcagtcgcgg tctgcacaca gacctcgcgc gcaatttgtt gacctatggt ttcgcggaag
     4201 agttggttga gaagatcaag atcgagtcga tcaagcagca actcgacgag gccattttga
     4261 atcgcctgca cgcgcggctc gaagtataac ttcagactta aatattgcga cttgggactc
     4321 ttaaaaaatt atgattgcca cagagaaatc tatgtcgggt gagacgggag caggcggaga
     4381 ggacgcggcg cagacggtgt cggcgtggga tgtggcgcga attcgtgaag attttccggt
     4441 gctgcgtcag accgtcaacg gcaaaccgct cgtctatctc gataacgccg cttcttcaca
     4501 agtgccgcaa gtcgtgattg atcgcggcag ccgttatctg cgtgaagaac actcaaacat
     4561 ccaccggggc gtgcattacc tgagccagcg cgcgacgagc gcttacgaag gggcacgcga
     4621 gaaggtgcgc ggcttcatca acgcacgcga gtcgcgtgag tgcatcttcg tgcgcggctg
     4681 cacggaaggc atcaatctgg tgatgcacgg ttacgggcgc aagttcgtca cagagggcga
     4741 cgagatcatc gtctctgcga tggagcacca cgcgaacatc gtcccctggc agatgctctg
     4801 cgaagagaag ggcgcgcatc tgcgtgtcat tccgatgaac gacgcgggcg agttgctgct
     4861 cgacgattat gaagccttgt tgaacgaacg cacgaaactc gtcgccgtga tgcacgtctc
     4921 aaacgcactc ggcacgatca accccatcaa agagatcgtc gcacgcgcac acaagcaggg
     4981 cgtgcccgtc ctcgtcgatg gggcgcaggg cgcgccgcac ctcaagatcg acgtgcagga
     5041 tttagatgcg gacttctaca tcatctccgg tcataaaatg tttgcgccga cgggcagcgg
     5101 cgtcgtctac ggcaaggcgg agatgctgga acggatgaac cctttcctcg gcggcggcga
     5161 catgattcgt accgtcactt tcgaaaagac gacttacgcg ggcttgccga acaagtttga
     5221 agcgggcacg cctgccatcg cctcgcagat cggactgggc gcggcgatag attatttgaa
     5281 cggaattgac cgcgccgccg cacacgcata cgagcaggag ttgctcgcct atgcgagcga
     5341 aaaacttact gctatcgaag gcgtgcgcct ggtcggcacg gcgcgcgaga aagtgggcgc
     5401 gctctcgttc gtcatcgaag atgtgcaccc gcacgatgtc ggcacgatac tggatcagga
     5461 aggtatcgcg atccgcgccg ggcaccattg cgctcagcct gtgatgaagc gtttcaacgt
     5521 cccggctacc gcacgcgcat ccttctcttt ctacaacacg aaggaagagg tcgatacgct
     5581 cgtgcatgcc gtgcagaaag taatcgaggt gtttagttaa agtgtcagaa ctgagcgagc
     5641 tatatcaaca ggtcattctc gaccacaata agaagccgcg caacttcaag aaattggaga
     5701 cggccaaccg tcaggccgaa ggcttcaacc cgctctgcgg cgaccagttg aacgtctacc
     5761 tgcaactcga agacgagcgc attacggatg tcagcttcga aggttcgggc tgcgccatct
     5821 caaaagcctc cgcgagcatg atgacgcaga gcgtaaaagg gaagacgcgc gaggaggcgg
     5881 agacgctctt tcaggaattt catcagatgg tgacgggcgc actcgacgaa gaggcgacgc
     5941 cgaatagtct cgggcgtctg acgatctttg cgggcgtgcg cgagtttcct gcacgcgtca
     6001 aatgcgcgag cctctcctgg cacacgatgc atgccgcgct ggccggagaa gaaattgtga
     6061 cgacggagaa tctggggccg gacatcacct cggagcaaat ttcagcctaa agagataagt
     6121 gcccttgcgt tgcagcacga ggctctacat agttcgtcgc taagctatcc aagtttcctc
     6181 acctcatctt taatcccaaa tgcctctctc ctgaacgcac ggaaaagcct gtgtgatcgg
     6241 tttgcacgcc cggctgcttt gcgtgattgg cggggcgtca ggcagggaag gagtaagtct
     6301 ggatgtattg aggcggcgac gcttccagga gggggcgtcc gtcggcttcg agccaagcgt
     6361 gccctgcgag gagcgactct ttttctttgc gcaggccgaa gacgatgcgc gtctcggtgc
     6421 cattgagcgc gagataacga tgcaggacag gcgcgcgttt ccaacaggtc ggcgtgaagc
     6481 acaggacgtt caggccgagc agtgcatcga ctaactgcgc caatctctgt tgagtaatcc
     6541 tgctgttctt tggcttttgg ctgcgtccgc tggcggcgac gagagagaga actcgcggga
     6601 gcgggagaaa tttaatgaga agcgaaagca gcacgatcca cgtcgccatg cgcagaagca
     6661 agaaggcttc gagcggcctg cgcttgagga atcgtcctgc acggcgcacc gttcctgtcc
     6721 gctgcatcca aaagtttcta ttttcaatcg gcatcagagc ttaatccatt acttgagccg
     6781 ccaccgctct tctttaagaa gataataagt ttagatgcga agctgtttgt tgttactgca
     6841 agaaatggcg tctctacggt tcagccggaa ggcgacaaag tttacgaaaa aggttcttaa
     6901 tgccccaccg cttgcggtgg ggatgaatga accatcttgg gggcgggttc gatgcccgcg
     6961 cacaagatag ctgatgttga tagaatgcgc gcatggtctt taggagagct gcgcacgcc
//
LOCUS       DASWNS010000372         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_248153, whole genome shotgun sequence.
ACCESSION   DASWNS010000372 DASWNS010000000
VERSION     DASWNS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248153"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VGB76_13075"
     CDS             complement(<1..482)
                     /locus_tag="VGB76_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099873.1"
                     /translation="MKTRQQRMSLTSVRPKNRTIFLFVCLSLLMLSAAPVTAQTICTP
                     QNPTTFNEPPPPTLPTAAGGTFCDPVFGTKIMRVTNETTAPGGAGTSYSYWPTFNQNN
                     TKILVVNPLAPHQGDIYDFNPTTFTLGDRLPAMPYVPDVGQTTKLDDAVWSYTEPNKL
                     "
     gene            complement(819..1817)
                     /locus_tag="VGB76_13080"
     CDS             complement(819..1817)
                     /locus_tag="VGB76_13080"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HEY0099874.1"
                     /translation="MKHTITLIPGDGIGPEVTAAVVRIIEAAGVEVEWETHYAGAQAL
                     EKFGETLPADLLESIQRNKVALKGPITTPVGKGFTSVNVGLRKALDLYANLRPVRALP
                     NVPSRYPELDLVVVRENTEDLYSGIEHVVVPGVVESIKIITEKASTRIAKYAFEYARI
                     EGRKKVTAVHKANIMKLSDGLFLDCFRKVAEGYPEIEADDKIVDNACMQLVMRPEQFD
                     IMLLENLYGDIVSDLCAGLIGGLGLVPGANIGERGAVFEAVHGSAPDIAGQGIANPTA
                     LLQSGLLMLRHLGERDAAQKIEGAMLKVFTEGKVRTRDIGGHAGTMEFAEAIIAAF"
     gene            1987..>2561
                     /locus_tag="VGB76_13085"
     CDS             1987..>2561
                     /locus_tag="VGB76_13085"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0099875.1"
                     /translation="MRNDGDELPESADVAVVGGGVIGLAVARELGRRGARVVLFERGE
                     HAGAEASWAAAGMLAPQVEADDADAFFAFASASRDAYPDFARELEEESGIDIEFDRTG
                     TLYLAFTEADEAECEHRFHWQRRAGLEIERLTADEVRALEPHVSARVRSALRFPRDGQ
                     VENRRLTAALIEACVAHGVRLFTQTEVTSIRV"
BASE COUNT          446 a          705 c          868 g          542 t
ORIGIN      
        1 aatagtttgt tcggctctgt gtatgaccag acggcatcgt ccagctttgt cgtctggcct
       61 acgtccggca cgtaaggcat cgcggggaga cggtcgccga gcgtgaaggt cgtcgggtta
      121 aaatcgtaga tgtcgccctg atgcggagcc agcgggttga cgacgaggat tttcgtgttg
      181 ttctgattga acgtcggcca gtaactatag ctcgtgcccg cgccgccggg cgcagtagtc
      241 tcgttggtga ccctcatgat cttcgtgccg aagaccgggt cgcagaacgt cccgcccgct
      301 gccgtcggga gcgtcggcgg gggcggctcg ttaaacgtcg tagggttctg gggcgtgcag
      361 atggtctgtg ccgtcaccgg agcggcggac agcatcaaga gcgacaggca gacgaacaag
      421 aagatcgtcc ggtttttggg gcgtactgaa gtgaggctca tcctctgttg acgtgttttc
      481 atgattaaac cttccctgtt gaagagtgtg agagtagcgg tctacgacgt gttcttcctg
      541 ttaaagcggg ttgggcttta gcgggcgtta acgtccgggg ttatcgagtt agcgggagga
      601 aacgggagtg agccggagtc tatcacgacg cctgagaggt gtccatcgtt gccctacacg
      661 cggctggagt cagttgcgat tgatcccgtt tgcgcgggat tggcgcagag agagagagag
      721 agagagagag agtgtggagg cgcgtgcgcg ttggctgcgc ctcctccacg tctgcggcgg
      781 tgaggtttaa tttttaagtc gcgcggtggt gaggcgcgct aaaaggctgc gatgatggct
      841 tcggcaaact ccatcgtccc ggcgtggccg ccgatgtcgc gcgtgcgcac tttgccctcg
      901 gtgaaaactt tgagcatcgc cccctcgatc ttctgcgccg cgtcgcgttc gccgaggtgg
      961 cgcagcatca ggaggccgga ttggagcagc gcggtcgggt tggcgatgcc ttgaccggcg
     1021 atgtcgggcg cgctgccgtg gacggcctcg aagaccgcgc cgcgctcgcc gatgttcgct
     1081 ccgggcacga gtccgagtcc gccgatgagt ccggcgcaga ggtcggagac gatgtcgccg
     1141 tagagatttt cgagcagcat gatgtcgaac tgttcgggac gcatcacgag ttgcatgcag
     1201 gcgttgtcca cgatcttgtc gtcggcctcg atctccgggt agccttcggc caccttgcgg
     1261 aagcaatcga ggaaaagccc gtcggacaac ttcatgatgt tggccttgtg tacggcggtc
     1321 actttcttgc gcccctcaat gcgcgcgtac tcgaaggcat acttggcgat gcgcgtggaa
     1381 gccttctccg tgatgatctt gatgctctcg acgacgccgg gcacgacgac gtgctcgatc
     1441 cccgaataga ggtcttccgt gttttcgcgc acgactacga gatcgagttc gggatagcgc
     1501 gaaggcacgt tcggcaaggc gcgcacgggg cgcaggttgg cgtagaggtc gagcgccttg
     1561 cgcaatccga cgttgaccga cgtgaagcct ttgccgacgg gcgtggtgat tggacctttg
     1621 agcgcgacct tgttgcgctg gatggattcg aggaggtctg cgggcagcgt ctcgccgaac
     1681 ttttccagag cctgcgcgcc cgcgtagtgc gtctcccatt cgacctctac gcccgccgct
     1741 tcgatgatgc gcacaaccgc cgccgtgact tccggcccga tgccgtcgcc cggaatcagt
     1801 gtgatagtat gtttcatagt cagaatatag aaaacaggag tcagaactca gcgttaagaa
     1861 gtcgtcgtcc agaaggcggg agcatttggt agcagagctt tcgcaaactc gaaccttctg
     1921 actcctgttt tctgtattct gacttctgct ttaaatttct tgcatcataa cagcaactct
     1981 aattcaatgc gaaacgacgg agacgagtta ccggagagcg cggacgtagc ggtcgtgggg
     2041 ggcggcgtga tcgggctggc cgtggcgcgc gaactcggaa ggcgcggcgc gcgcgtggtc
     2101 ttgttcgagc gcggcgagca tgcgggcgcg gaggcttcgt gggcagccgc cgggatgctc
     2161 gcgccgcagg tggaggccga cgacgcggac gctttcttcg ccttcgcctc cgccagccgc
     2221 gacgcttatc cggactttgc acgcgaactc gaagaagagt cgggcatcga tatcgagttc
     2281 gaccgcacgg gcacgctcta tctcgctttc acggaagcgg acgaggcgga gtgtgagcat
     2341 cgcttccatt ggcagcggcg cgccgggctt gagatcgaac gcctgacggc agacgaggtg
     2401 cgcgcgcttg agccgcatgt gtcggcgcgc gtgcgttcgg ctctgaggtt tccgcgcgac
     2461 gggcaggtgg aaaatcgtcg tctcacggct gcgctcatcg aagcctgcgt cgcgcacggc
     2521 gtgcgcctct tcacgcagac ggaagttact tcgatcagag t
//
LOCUS       DASWNS010000373         3774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_403325, whole genome shotgun sequence.
ACCESSION   DASWNS010000373 DASWNS010000000
VERSION     DASWNS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403325"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..454)
                     /locus_tag="VGB76_13090"
     CDS             complement(212..454)
                     /locus_tag="VGB76_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099876.1"
                     /translation="MNGLRVYINPAGASAQNGQRVFYSRRADGPYYRWHYQDEFGRWH
                     GSRVHLSNLTLRVLSIASWESVPKALQVKLGEHYLE"
     gene            complement(451..768)
                     /locus_tag="VGB76_13095"
     CDS             complement(451..768)
                     /locus_tag="VGB76_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099877.1"
                     /translation="MSAINSETFSQICEGVWRNRASILAGRGNCSGEVALLRAVYWRL
                     CKVGGESGQVSDDCDANHLLPAYQQLIGRTLAGHASPRFDGTPFLDELLRRYREEVEA
                     AKR"
     gene            complement(774..1058)
                     /gene="rpoZ"
                     /locus_tag="VGB76_13100"
     CDS             complement(774..1058)
                     /gene="rpoZ"
                     /locus_tag="VGB76_13100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY0099878.1"
                     /translation="MNVTEQIAIKGYHDDTNADEWPGIDSRFRLIVVAALRTKQLLHG
                     ARPRIEADPLKRRNTSIALEEVRQGLVSFTNNSKDAVGNGDGAAEQKSDG"
     gene            1382..1573
                     /locus_tag="VGB76_13105"
     CDS             1382..1573
                     /locus_tag="VGB76_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099879.1"
                     /translation="MPIHVRHKSFAPRRPAPSHCRHARAGCVTETLPFPPHTRQEITR
                     LADVAERPVPSHHAHLSGA"
     gene            2027..2569
                     /gene="msrA"
                     /locus_tag="VGB76_13110"
     CDS             2027..2569
                     /gene="msrA"
                     /locus_tag="VGB76_13110"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY0099880.1"
                     /translation="MEQREERATLAGGCFWCLEAVFADLRGVARVVSGYAGGDVPNPT
                     YQAVCSGTTGHAEVVQVTYDPQVVSFAELLEVFFTIHDPTTLNRQGGDIGTQYRSAIF
                     YHSPEQREIAGRVISEFDAERVWNAPIVTEVVPLTVFYAAEDYHQEYYRNNQNQPYCR
                     AVIAPKVSKFRQKYLEKLKR"
     gene            complement(2707..3279)
                     /locus_tag="VGB76_13115"
     CDS             complement(2707..3279)
                     /locus_tag="VGB76_13115"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY0099881.1"
                     /translation="MKTLRNSLVTLAVFFALVVSTVSVGASPLAESDQALEHQQVSKR
                     VRKELVTLPYYGVFDNLAYEVEGGTVTLHGQVVRPSTRSDAARRVAKIKGVERVVNNI
                     EVLPLSSFDDRIRLQAYRSIFNTGGLYRYAMGTNPSIHIVVNRGHLTLEGVVSSRMDK
                     QLAEFAARNVFGVFSVTNNLRAESEERSRG"
     gene            3564..>3774
                     /locus_tag="VGB76_13120"
     CDS             3564..>3774
                     /locus_tag="VGB76_13120"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY0099882.1"
                     /translation="MASVWELGGMTWRELGRRVWCEIYDGDLLTRAAALSYYFLLALF
                     PLLLFLIAMLGYFAEAGTELRGDLLR"
BASE COUNT          789 a         1095 c         1090 g          800 t
ORIGIN      
        1 ccgacgacga ggaaactttg aatccattga atccgagaca gcagggagag aggctgttcg
       61 agagcgagtt cgcgagcggg ttctttgagc aaaaactttt ccttgaatta ttttgagcac
      121 tgcacgctga tactatacac acaccacaat cgagcaggct aacgctccga ttaaatttca
      181 ttgcccggcg cgttccaaat atcgccgcct gttattcgag ataatgctct cccaacttga
      241 cctgcaaagc cttcggcacg ctctcccaac tcgcaatgga aaggacacgc aaagttaaat
      301 tcgagagatg cacgcgcgag ccgtgccagc ggccaaattc atcttgatag tgccagcgat
      361 agtaaggacc atcggcgcgg cggctgtaga agacacgctg gccgttctgc gcgctcgcgc
      421 ccgccgggtt gatatagacg cgcagaccgt tcatcgcttc gcggcctcga cttcttctct
      481 gtaacgccgc aaaagttcat cgaggaaggg cgtgccgtcg aaacgggggg aggcgtgccc
      541 ggcgagcgtg cgaccgatga gttgttgata ggccgggagc aggtggtttg catcgcagtc
      601 gtcgctcacc tgtccggatt cgccgccgac tttgcagagt cgccaataga cggctcgcag
      661 cagagccacc tcgccgctac aattgccgcg tcccgcgaga atgctcgcgc ggttccgcca
      721 caccccttcg cagatttggc taaacgtctc tgaattgatc gcgctcatct ttactacccg
      781 tccgacttct gctctgccgc gccgtcgccg ttccccaccg cgtccttgga attattcgtg
      841 aaggagacga gtccctgcct gacttcttcg agggcgatgc tcgtgttgcg acgcttcaac
      901 gggtcggctt cgatgcgcgg gcgcgcaccg tgcaacagtt gtttcgtgcg tagagccgcc
      961 acgacgatca ggcggaaacg agaatctatc cccggccatt catccgcgtt cgtgtcgtcg
     1021 tgataacctt tgatggcgat ttgttctgtg acattcataa gaagatgctc cggctcgcgc
     1081 atttgtgtcc gtctgcacgg agggtaaatt gccgcgagaa acaatcacca ctgaatagtt
     1141 aaaaaactct aagcaggtaa aagacggcca gcgccgcgac caccgtaatc gcgagcacgt
     1201 gcggccaatt caacgtcgat tgatggcaat aaggacaacg catgtcccac agacgcaacc
     1261 cgcccccaca ctcgtgccga tactctctca tttcagtttc ctgatccggc ctctggctct
     1321 gcttcaccag acaccggctc gcatccctgg ttcgtaaatt taaactatag gtgaccaacc
     1381 cgtgcccata cacgtcaggc acaaaagctt tgcgccgcgc cgccctgcgc cttcgcattg
     1441 caggcacgcc cgcgccggtt gcgtcacgga gacgttgccc ttcccgccgc acaccagaca
     1501 agagatcacg cggctcgccg atgtggccga acgccccgtg ccctcgcacc acgcgcatct
     1561 ctccggcgca taactctttt tcttcacagt agtgctcatc tgccatcacc accacgcgaa
     1621 agatgcgcgc gacacgagac ggaacaggca gcgatcaggc gtccgcacag ggaagagttt
     1681 cgatggcgaa ggggcgaatg aattttagcg tgtgattgat gcaagaatca ggagcctccg
     1741 tgtgcagtta gcgtgtcgaa aaaagaacag acggcacaag gagcagtgcg gctcgcgcaa
     1801 atcctgatgt cgagagtaga tgttttccaa agagcagcct ttacatgacg tgtgaagtat
     1861 agcatagatg aaggccggac gccctcgcac tgctggcctt cgcacgcatg agcgacacgt
     1921 tgcgcgcgtc gggtcagcgt tggaacgcat gttaggatga agacatctcc agcacgtcaa
     1981 ctacaatcta agccttatct tcgagactat taaaaggagc gtgatcatgg aacagagaga
     2041 ggagcgcgcc acgctggccg gaggatgttt ctggtgcctg gaagccgtct tcgcagatct
     2101 gcgcggcgtt gcgcgggtgg tgtcgggcta cgcgggcggc gacgtgccga acccgactta
     2161 tcaggcggtc tgtagcggca cgaccggaca tgcggaggtg gtgcaggtaa catacgatcc
     2221 gcaggtcgtc tcttttgcag aactgctgga agtcttcttc accattcacg acccgacgac
     2281 tttgaaccgg cagggcggcg acatcggcac gcagtatcgt tcggccatct tctatcattc
     2341 gccggaacag agagagatcg ccgggcgtgt gatctcggag ttcgacgcag agcgcgtctg
     2401 gaatgcgccc atcgtcacgg aggtcgtgcc tttgacggtc ttctacgcgg cggaggatta
     2461 tcatcaggag tattaccgga acaaccagaa ccagccctat tgccgcgccg tcatcgcgcc
     2521 gaaagtctcc aagtttcggc agaagtatct ggagaagttg aagagataga cgcgaggcgc
     2581 gggcgcgagg gagatcggcc tttacacgcg cacgaaaaag cagaagagcc gcgcggactg
     2641 ttcgccgcgc ggctcttcta tttatgttct cgtgtttatg ttcgcgcctt tgcggaagcg
     2701 aactctttac ccacgactac gctcttcgct ctcggcgcgc aggttgttcg tcacggagaa
     2761 gactccgaaa acgttgcgcg cagcaaactc cgcgagttgc ttatccatcc gcgaactgac
     2821 gacgccttcg agcgtcaggt gtccacggtt gacgacgatg tggatcgacg gattcgtgcc
     2881 catcgcgtaa cgatagaggc cgcccgtgtt gaagatgctg cgatacgcct gcaagcgaat
     2941 acggtcgtcg aaacttgaga gcggcaacac ttcgatgttg ttgaccacac gctcgacgcc
     3001 cttgatcttc gccacgcgcc gcgctgcatc cgagcgcgtc gaaggccgaa ccacctgccc
     3061 gtgaagcgtc acggtgccgc cttcaacttc gtaagcgagg ttatcgaaca cgccgtagta
     3121 aggcagcgtc acgagttcct tgcggacacg cttcgagact tgctggtgct ccaacgcctg
     3181 atccgactcc gcgagcggac tcgcgcccac gctcaccgtc gaaacgacca gcgcgaagaa
     3241 gaccgccaac gtgactaaac tgtttctcaa agttttcata gctcttattt cacccctcga
     3301 taattgttct ctcactctat ttatttgcga gaattagatg cttgagagct tttacgcgtg
     3361 gcctccgcgt gcgcggcgtg cgcgaggttc cctgcacgtt tttaggcgcg cgcgaaggtt
     3421 cggcgacgga ctgtgattag aactattttt tgctattgtt agcaggcgcg tgatcgtcgt
     3481 ttttgcgaga gaggtgtcgc acgtgtgcgg gcgcgcacgg gtcagttaag gggcgggaaa
     3541 gagcaggaag ggggcggggc gagatggcat cggtctggga actggggggg atgacctggc
     3601 gcgagttggg gcgtcgcgtc tggtgtgaaa tctacgacgg cgatctgctg acgcgtgcgg
     3661 cggccttatc ctactacttt ctgctcgcgc tctttccgct gctgctcttc ctgatcgcca
     3721 tgctcggata tttcgccgaa gcgggcacgg aactgcgcgg cgacttgctc cgtt
//
LOCUS       DASWNS010000374        15308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_482674, whole genome shotgun sequence.
ACCESSION   DASWNS010000374 DASWNS010000000
VERSION     DASWNS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15308
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482674"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1919)
                     /locus_tag="VGB76_13125"
     CDS             complement(<1..1919)
                     /locus_tag="VGB76_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099883.1"
                     /translation="MSQETPEVYPSHSDTAEFGDQPPSGRNAASWLTAIIESADDAII
                     SKTLDGIITSWNDGARRIFGYMAEEVIGKPVLILIPPEHQDEEPNILARIRRGERIEH
                     YETLRMRKDGTLVDISLTVSPIKGADGRIIGASKIARDISERKRAEALLREQREIIEI
                     VNRVGQSVAAELDLHKLVQTVTDAATEISGAHFGSFFYNVLDEKGASYMLYTLSGVPR
                     EAFAHFPMPRATDLFGPTFRGEGTIRIDDVKLDPRYGKNSPYYGMPEGHLPVTSYLAV
                     PVVSRSGEVVGGLFFGHPEAGVFTERVARVVEGLAAQAAVAVDNASLYDAAQRARAEA
                     EQVAEEKQRLYIEAQESNRLKDEFLATVSHELRTPLTAILGWAHLLRTGQTVDGKMTK
                     ALETIERNARAQAQLIDDLLDVSRIITGKIRIDVRPIDPNSFIEAAVEAVRPAAEAKG
                     VRLQRIMDTGVATVSGDPVRLQQVVWNLLSNAIKFTPKGGRVQVRLERVNSHIEIGVS
                     DTGPGIEAEFLPYVFDRFRQADGRTTRQHGGLGLGLAIVRHLVELHGGTVRAESAGEG
                     QGATFTVLLPVAPVYQTVGAVERVHPAARDTLPLYECPDRLDGLRILVVDDEPDTREL
                     LKAGLGQCGAVVTVVG"
     gene            2269..2559
                     /locus_tag="VGB76_13130"
     CDS             2269..2559
                     /locus_tag="VGB76_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099884.1"
                     /translation="MADLIGNVQGETTPSVTGVEVIGELKDDYAVHVFFEERDEGFWF
                     APEILEFVDHAAGTELRLDGVAKKWVRSESGEWVESEVEGAGQTKNPWWKFW"
     gene            2693..3073
                     /locus_tag="VGB76_13135"
     CDS             2693..3073
                     /locus_tag="VGB76_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099885.1"
                     /translation="MAFILLFVSLSAGCADKHEPRAGTVVLSYQELGPSVVVYELIGS
                     EWYQWNSHGGSDPTEIDDVKVVVYRNVSLDKVKAMYPVVTGKQDYRYLDYEAAIKYLN
                     KNEGEPYLKHLQATKRKIIEQLGS"
     gene            3221..4681
                     /locus_tag="VGB76_13140"
     CDS             3221..4681
                     /locus_tag="VGB76_13140"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0099886.1"
                     /translation="MWNLSKMFMRTRTVRFASRISALLLLTVLSTGVLRAQASFDVNQ
                     QLSPEQLIADVDFYLKTLEEAHANPYAQISAKEFRARADEIKSRIRRQGAMTQKEFWL
                     LFTRLVSAIQDSHSYVVDPRFFVKPEDDTTKYFPLRTVYIDGKIVVTASFADEKIEKG
                     AVIEAINGTSSKELIRKISEHRFGVERERVESAVLWLWVGAAEIFGRPSEFTVTFADG
                     RKVQVRGLRLPEFIARENAARSANPSAAKNNSPLELKLLDGGVAYLNSTTFEYDLEEY
                     RALLKDVFTQIRSSGVERLIIDLRNNTGGNSALGDALIGMFNSKPYRHYSMRWKRSVQ
                     YVEEMKRKKTPLPDNYLALRPGEMLSGESQVVRPGDNPLRFKGRVYVLSSKETFSSGQ
                     MFLAVVKDNGLAKVIGEENNEPVCSYGEVFFFNLPNSRLRTSLSVKYFTPPAGCKDAR
                     GVVPDIPVKRRVADYVTGRDAILEATLNLIKREGDN"
     gene            4787..5290
                     /locus_tag="VGB76_13145"
     CDS             4787..5290
                     /locus_tag="VGB76_13145"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0099887.1"
                     /translation="MSETKKSERTLTVSWEDPQGLAEAGRGLSGLEYLQKIVAGELPR
                     PPISALMNFSLSELSEGYAVFAVEPAEYHYNPIGVVHGGLAATLLDSAMGCAVHSMLP
                     AGAGYTTLEFKINFVRAMTTETGRVRCEAKLIHLGGRTATAEGRIIDEDGKLYAHGTT
                     TCLIFRS"
     gene            5560..7908
                     /locus_tag="VGB76_13150"
     CDS             5560..7908
                     /locus_tag="VGB76_13150"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099888.1"
                     /translation="MKSRVGLKQIYIFFLLILNCNVLVAPAQTRRTGTRQGTQAAAAA
                     PTKAKAQSCSGAWTGVINYTRTQTNSNNKTVQRVSGRGEDTTDWQMNYNYKAAVAVVE
                     SPEKNGTSAGKASINHTFSSTETVSARERNSCDRGKTWRVMTGTTTSKTETKASAGGL
                     EANVHIGVNADGTYTVSVALPQIQGKTTGSQSSTYGGQCVPKEGKNLSLPPTDTSVDG
                     NSLTSDGSHRVNASDPNRLSGSYSKTWQNVTETITWSLQKCGAPLRLIDLKFEHPKYP
                     NFNDWQEFAEQTGTVDGNMVKIKARVLNASGETKFADVKFKETYKGDKWDGARPDAPL
                     EDTAVSVRLEPGEEREVEMVWDSSGYAWFDDGRPRLVQRIKAELEESGKKVDELTKNL
                     KVAPKPLVLVHGLWSNWRAWESWQNILTTSHSYDWKAFPVGEKPEKGLMNTGGEFLSS
                     GPTNGIFENSQQLGKYIRYAQEERNAWHVDIVAHSMGGLISRHYIHNFMPVDSPDGRP
                     QVAHLVMLGTPNMGSPCADVMNSAFDLLGKNVEAIRQLKPSVVAEFNRVNVNRKGAKF
                     SVLAGNPLPVMCKTVVLNDGVVPVESAHWLIKDNALSKNLHTDLTGTADFSSFVKPRL
                     AIGPKGNHNPEPPELPRNSNQTGRNFDNPYAAMFVNASYRPQSSSVAGVGDDLRPAFA
                     KEVKLAPKQSLEIELNVPAGANFGVTFMSNPKVSATLFDDKGAVQGKNLADSPESRGL
                     FRTIFVERGITAGTWKLKVENSGEQEAVAVIAAWSDAVKMQR"
     gene            complement(7996..10839)
                     /locus_tag="VGB76_13155"
     CDS             complement(7996..10839)
                     /locus_tag="VGB76_13155"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0099889.1"
                     /translation="MTAHTGAQHPAVAGDDPTPRTQPRASRTQPRASRTLAAVIFYAL
                     LSLIVLAPAKYGLVREWWVSLFQCVVFALAALWALEGLLGGGWFVREHRLLAPLVPLL
                     LFVFLQSVPLGREEVAGVEVWRTLSADAYETRLAAFRFLAHLLVAAMLLRYTSNRRRL
                     AALICAVLVAGVSSALFGLARRALHSDGEPFASPYLWQQLEYAPFNANHFALLLEMCL
                     GLLLGLAAGAGALPRGLRLFLCLPAAALLWAALVLSTSRGGIFAAAGQIVFLALLWGV
                     LTPRRRRHDEEKTAVTRRWFAHPLIVRWTATTCLLVALGFAAVWIGGDILMQRMESLR
                     GEIGAQGAGTRDFPRRSRIWWATWQLIKEHPLAGTGFGGYWLAITPFFDASGIGLLEQ
                     AHNDYLELLASGGLVAIAAAALFGGLFIQRARECLRSGDPFRRAACCGALTGLCGVAL
                     HSFVDFGLHITANAHLFTALIVIAAAHVCADKRVQPARAVHAQDRAVRRSLKDLFQRA
                     SDGRVVRVASVALCLLCCGALMWATARAGLSRRYSVGQAHDYSLSLAERAIRLSPHDP
                     TARYFRAELLLASGRNDAAREELQRAAELRPRLYSLWLRLGLVREAGGDLRGAMAALQ
                     EGVRLAPFYAEPRWVLGGALLRAGERERAFAEFSRAVASDPDFPPQALELLWEASGGD
                     ADAMAQAISPHTATARTALARFFVKRGAASGAAAALLRQAGHDAGEARRVITSELIAA
                     KKFREAYDFWSSGRVGGAGAGRGEAGFITDGGFEGEINPQEFGFGWRVEASPGGLTVS
                     SDTQGARAGARSLRLDFNGEGWAQSPLISQMVLVEKDSRYRLSFAARVQGMKSVWPPV
                     LSMRDADSGEELASPIPLRGKTTGWQEFNVEFKTATTTAAVLFAIERQQCAAPPCWHT
                     GRIWLDEFSLQKLTAPKAALLTGGVAIATAGRR"
     gene            complement(10956..13073)
                     /locus_tag="VGB76_13160"
     CDS             complement(10956..13073)
                     /locus_tag="VGB76_13160"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEY0099890.1"
                     /translation="MKVLKLLIIAIFAAALIATIAAPTRTAHGQTEAPAGFDNQTNGF
                     TDQATFDADRAAFEKREERINGLGPVYNAQACSECHQNPVTGGISQLFELRAGRGLNP
                     VTTTSNPPVFSPSRQTVPAPLTPLSFFPAPGGSLIQARAVDPSIQERVADGPRIVFMG
                     DNQLISVMGFDGGQYGVVGNSPVTGVYPAFSPDGTQIVFQRSVGGNINIFVMNNDGTN
                     LRQLTNTGVDMSPAWSPNGTKIAFGSRRTGIWQIFLMNPDGTNQVNISNSNANDSWPA
                     WSSNSGSITFTRSQPTMTPPFEKIWTMSSSGTGQTQITFGAGNDERPSFSPDNGSIVF
                     GSDRDGNWEIYKAPSSGGTPTRLTNHPADDTNPTWSPDNASIAFASTRNNATRIWAMA
                     TDGSTQTQISIAGGNYPAYSRDLDGDTVRTFRSSLNILGDGFVEAIDDATLLSIRDNQ
                     PVEMRGTAISVSLLEAPQQTRLGRFGWKNSVASLLSFSAGAYLFEMGITSPLLPTEQT
                     SLGRNVMAFDLTPDAEDTGGEQGFGDDVEAFTRFMRATKVPPRDRALVPDDSTDPGSQ
                     LFDQLSCSVCHVRSITTAPAGTVINGGTFVVPAALGDKIIHPFSDFLLHDIGTGDGIV
                     ETNGEATRLMVRTPPLWGLRTHDRLMHDGGSSSAPSNSGVQSFTLREAIMRHGGQATA
                     SRNAFQSLSLLQKAQLYKFLKSL"
     gene            complement(13839..15041)
                     /locus_tag="VGB76_13165"
     CDS             complement(13839..15041)
                     /locus_tag="VGB76_13165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0099891.1"
                     /translation="MTSKNVELAAVALLCDPNGVVQQVLYDGLGAGQIASAQPFESAL
                     DFESREKGRAFLEVVREKGAAFDWELNLAVGEQVRSIRFSGSATGDGIFIAGAATRSG
                     IIKVYEQFVHVVDSPSGNSTAQGWPGSAPAQLFDGDTELYNELARLNNELVTAQREMI
                     KRNIELERLNEVKNQFIGVAAHDLRNPLQVIEGYSQILLDKHFGELTAEQHKFISVIR
                     KNSDFMLNLITDLLYISKIEAGKLQLELKETDLAALLERNVELNRLMAGQKQINILFT
                     HREDLPALLIDAPKIEQVLNNLISNGIKFSHPGSKVEVWATKREREVVVSVRDEGQGI
                     PANEIDRLFIPFENLSVKSTGGEQSTGLGLAIVKRIVEGHGGSIWVQSESGVGSNFSF
                     SLPFIEPV"
     gene            complement(15060..>15308)
                     /locus_tag="VGB76_13170"
     CDS             complement(15060..>15308)
                     /locus_tag="VGB76_13170"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEY0099892.1"
                     /translation="NAILQALSERRADVLAVSATMTFHIRAVENLIAAVRASEDLKAV
                     KILVGGYPFNVEPELWRRVGADAYAADAADAVAMASSL"
BASE COUNT         3151 a         4439 c         4706 g         3012 t
ORIGIN      
        1 cctacgactg tcaccaccgc gccgcactgc cccagcccgg cctttaacaa ctcccgtgtg
       61 tctggttcgt catccactac gagaattcgc agcccgtcga gcctgtccgg gcactcatac
      121 agggggagcg tatctctcgc cgccggatgc acacgctcga ccgcgccgac ggtctggtag
      181 accggtgcga ccggcaacag caccgtaaag gtcgcgccct gtccttcgcc cgcgctctcg
      241 gcacgcaccg tcccgccgtg cagttcgacg aggtgacgca cgatggcaag ccccagcccc
      301 agccctccgt gctggcgtgt ggtgcgcccg tcggcctgac ggaaacggtc gaagacgtag
      361 ggcaaaaact ccgcctcgat cccggggcct gtgtcgctca cgccgatctc gatgtgcgag
      421 ttgacccgct ccagcctcac ttgcacgcgt ccccctttcg gggtgaactt gatggcgttt
      481 gagagcagat tccagaccac ctgctggagc ctcacagggt cgcccgacac agtggctacg
      541 cccgtgtcca tgatcctctg caagcgtacg ccctttgcct ctgccgccgg tctgaccgcc
      601 tcgaccgcgg cctcaatgaa tgagttcgga tcaatcggtc gcacgtcgat gcggattttg
      661 cccgtgatga tgcgcgagac atcaagcagg tcgtctatga gttgcgcctg cgcacgcgcg
      721 tttcgctcaa tggtttccag agctttcgtc atcttcccgt caacggtctg ccccgtgcgc
      781 agcaggtgcg cccagccgag aatggcagtc agcggcgtac gcagttcgtg cgagacggtg
      841 gcgagaaact cgtccttgag gcgattcgac tcctgcgcct cgatatacaa acgctgcttt
      901 tcctctgcga cctgttcggc ctcggcgcgg gcgcgctgcg ctgcatcgta gaggctcgcg
      961 ttgtcaacgg cgaccgccgc ctgcgcggcg agtccctcga cgacgcgtgc cacgcgctcc
     1021 gtaaagacgc ccgcctccgg gtggccgaag aacagcccgc cgaccacctc gccggagcgc
     1081 gagacgacgg ggacagccag ataactcgtg acgggcaggt ggccttccgg catgccgtaa
     1141 tagggcgagt ttttgccgta gcgcggatcg agttttacgt cgtcgatgcg gatcgtgcct
     1201 tcgccgcgaa aggtagggcc gaagaggtct gtggcgcgcg gcatggggaa gtgcgcgaag
     1261 gcttcgcgcg gcacgccgga gagcgtgtag agcatgtagg acgcgccctt ttcgtcgagc
     1321 acgttgtaga agaaggagcc gaagtgtgcg ccgctgattt cggtcgcggc gtccgtgacg
     1381 gtttgcacaa gcttgtgcaa atcgagttcg gctgccaccg actgcccgac gcggttgact
     1441 atctcgatta tctctctctg ttcacgcaac agggcttcgg ctcgcttgcg ctcgctgatg
     1501 tcgcgcgcga tcttggacgc ccctatgatc ctgccgtcag cccctttgat cggtgacacg
     1561 gtaagggaga tgtctacgag cgtgccgtcc ttgcgcatgc gcagcgtctc atagtgttcg
     1621 atccgctcgc cccgccggat tcgggctaga atgttcggct cctcgtcctg atgctccggt
     1681 gggatcaaga tcaagaccgg cttgcctatt acctcctcgg ccatgtagcc gaagatgcgg
     1741 cgcgcgccgt cattccaact cgtgatgatg ccgtcgagcg tcttgctgat gatcgcatcg
     1801 tcagccgact cgatgatcgc agtcagccat gaggctgcgt tcctcccgct cggcggctgg
     1861 tcgccaaact ctgccgtatc ggaatgagaa ggataaactt caggcgtttc ttgtgacata
     1921 ggcgaatgat tctactatct gctcgatgag atacgctttc gagaaaagtt tatgatgccg
     1981 cgattatata cgagcgatgg ggttattcct ataatcgtcc ctgaacaccc gcgtctgaag
     2041 tcgcattacc gtttgtgctc ccggatgaac ttctcttcga atgtacgcct ctctccgcgt
     2101 tttatttttc catagtgatt ttcctggtgt aaattgtcgc tggcacccgg cgcggggcaa
     2161 ttcagacgtt aggcgtcccg gtaatcgaac ctaatgcaag acgatcattc aatctcgttc
     2221 ggcgacaacg tccgcatccg agcaactgct ttgacgcagg aacggggttt ggcggacttg
     2281 atcggcaacg ttcaagggga gacgacacct tcggtcacgg gtgtcgaggt gattggcgag
     2341 cttaaagacg attatgccgt ccatgtgttc ttcgaagaac gtgatgaggg gttttggttc
     2401 gcgccggaaa tccttgagtt cgtagaccat gcggcgggca cggagttgag gctggacggt
     2461 gtcgcaaaga agtgggtgag atcagagtcg ggcgagtggg ttgagagtga ggttgaaggt
     2521 gcggggcaga cgaagaaccc ctggtggaag ttctggtaac gtcgccgcct gacaacagca
     2581 gacagccgac cgttgcgaaa attacctttg cacgcggaag gccgccagcc cggcacccgg
     2641 ccactaatta gcggctctcc ttgagatgga tatgagacga tcacagttgc agttggcatt
     2701 tattctgctc tttgtatctc tgtctgccgg ttgtgccgat aagcacgagc cacgcgcggg
     2761 cacagtcgtg ctgtcctatc aggagcttgg tccgtcggtg gtggtttatg aattgatcgg
     2821 gtctgaatgg tatcagtgga acagtcatgg cggctccgac cccactgaga ttgatgatgt
     2881 gaaagttgtc gtctatcgga atgtttcgct tgataaagtc aaagcgatgt atcccgtggt
     2941 gacggggaag caggattacc gctaccttga ctacgaggca gccatcaaat atctgaataa
     3001 gaacgagggt gagccttacc tgaaacacct tcaggcgacg aagcggaaga tcattgaaca
     3061 actcggctct taacgaaggc ttggcgcacc tgctcgcgcc gtctaacaac ggcgcacccc
     3121 ccgtcgcccc ttcacggagt tttcatgcac atagaatgtg ggcgcggacg ttgccgtgcg
     3181 tctggcgtca tagattactt gtgattggaa agtgtgaacg atgtggaatc tctctaaaat
     3241 gtttatgcgg actcgaacag tccgcttcgc ttctcgaatc agcgcgcttc tgctgttaac
     3301 cgttctgtct accggcgtct tgcgtgcgca ggcctccttt gacgttaatc aacaactcag
     3361 tcccgaacaa ttgatcgcgg atgtggattt ttaccttaaa acactcgaag aggcgcacgc
     3421 gaatccttac gctcaaatct ccgcgaagga gtttcgagcg cgggcagacg aaatcaaatc
     3481 ccgcatccgc cgtcaaggag cgatgactca gaaggagttc tggctgctct ttacgcggct
     3541 cgtgagcgcg attcaggatt cgcatagtta cgttgtcgac ccgcgttttt ttgtcaagcc
     3601 ggaagacgac acgacaaaat acttcccgct ccgcacggtt tatattgacg gcaaaatagt
     3661 agttacagcg tcattcgcgg atgagaagat agagaagggc gcggtgatcg aggccatcaa
     3721 cgggacttcg agcaaagagc ttatccgaaa aataagcgag caccgtttcg gcgtcgaacg
     3781 agaaagagtc gagtcagccg tcctgtggct gtgggtaggc gccgctgaaa tcttcgggcg
     3841 accgagtgag tttactgtca cgttcgccga cggcaggaag gttcaggtca gagggttgag
     3901 actgcccgaa tttattgcga gggagaacgc cgcccgctct gcgaatccgt cagcggcgaa
     3961 aaataattct ccgcttgagt tgaagcttct cgacggcggc gtggcctacc tgaactccac
     4021 gactttcgag tatgacctgg aagaatatcg agcgctcctc aaggacgtgt ttacgcaaat
     4081 ccgctcatcc ggcgtcgaga gactgattat tgacttaagg aataacaccg ggggcaattc
     4141 tgctctgggc gatgcgctga tcggcatgtt caattccaag ccttaccgcc attattcgat
     4201 gagatggaaa agaagcgtcc agtacgtcga agagatgaag cgcaagaaga cgcctcttcc
     4261 tgataactac ctggcgctgc gtcccggaga gatgctttcc ggcgaatctc aggtcgtccg
     4321 ccccggcgat aatccgctgc gcttcaaggg acgagtttat gtcctgagtt cgaaggagac
     4381 tttttcgagc gggcagatgt ttctggcggt ggtcaaggat aacgggttgg caaaagtcat
     4441 cggcgaggag aataacgaac ccgtatgcag ttacggcgaa gtattctttt ttaatctgcc
     4501 gaattcgagg ctgcgaacct ccctctccgt gaaatacttt acaccaccgg cgggctgtaa
     4561 agatgcgaga ggagtcgtgc cggatattcc agttaaaaga cgggtcgccg attatgttac
     4621 ggggagagac gccattttgg aggccacgtt aaatttaatt aagcgagagg gcgataatta
     4681 aaggttcgaa gttgggagtt ctgagtcggg agttcgtgct ctcacgctgc aagaacacgt
     4741 cccaagttgg acgccgggtg gcgaacgtct ccgttcggga atgattatga gcgagacaaa
     4801 aaaatcagaa cgcacgctca ccgttagttg ggaagacccg caggggttgg ccgaggcagg
     4861 ccggggttta tccggcctcg aatatttgca gaagatagtt gcgggcgaat tgccgcgccc
     4921 tcccattagc gcgctgatga atttcagcct gtcggagttg agtgaagggt acgccgtctt
     4981 cgcagtcgaa ccggcagagt atcactataa tccgattggc gtcgtacacg ggggcttggc
     5041 ggctacactg ctcgactcgg cgatggggtg tgccgttcat tcgatgctgc ccgcgggggc
     5101 aggctacaca accctggagt tcaaaataaa cttcgtccgg gcgatgacga cggagacggg
     5161 gcgtgtgcgc tgcgaggcga aactgattca cctcggcggg cggacggcga ctgccgaagg
     5221 ccgcatcata gacgaggacg gaaaacttta cgcccacggc acgaccacct gccttatctt
     5281 tcgctcttaa gaagaactac tgtgctaaac cgcttgttac ggcacactag tagttcgtct
     5341 caaacctctc tcagagcgca gcataaagag atagactcaa ggaatctttc gcccctcaaa
     5401 attatctctg taagactccc ccggccaacc aaaccggaga cggtaacggg tcttcgaatg
     5461 aaagccgcgt tcaatagttg atccaaaagg ctctgagttt tcgcgttaat gtttagccgg
     5521 aattttttcg gccactcttt aactacaagg tgatgaatta tgaagtcgcg cgtcggctta
     5581 aaacaaattt atatcttttt cctgctgatt cttaactgta acgtcctggt ggctccggcg
     5641 cagacgaggc ggacgggaac caggcaaggc acacaggcgg cagccgccgc cccgacgaag
     5701 gcgaaggcac agtcctgctc gggcgcatgg acgggggtga ttaactacac ccggacacag
     5761 actaactcga acaacaagac cgtgcagcgc gtctccggca ggggcgagga cacgaccgac
     5821 tggcagatga attacaacta caaggctgcg gtcgccgtcg tcgaaagccc ggagaagaac
     5881 ggcacgagcg cgggcaaggc ttcaataaat cacaccttta gttctacgga aactgtctcg
     5941 gccagggaga ggaactcctg cgaccggggc aagacgtggc gtgtgatgac cggcaccaca
     6001 acctcgaaga ccgagacgaa ggccagcgca ggcggtttgg aggccaacgt tcacatcggc
     6061 gtcaacgccg acggcactta caccgtcagc gtcgccctgc cccagataca gggcaagacc
     6121 accggctcgc agagttcgac ctacggcggg cagtgcgtgc cgaaggaggg gaaaaatctc
     6181 agcctgccgc cgaccgacac gagcgttgac ggaaactctc tgacgagcga cgggagccac
     6241 cgtgttaacg ccagcgaccc gaaccgcctg agcggcagct actcgaagac gtggcagaac
     6301 gttacggaaa ccatcacctg gagcctgcaa aagtgcggcg cgccgctgcg cctgattgat
     6361 ctgaagttcg agcacccgaa atatccgaac ttcaacgact ggcaggagtt cgcagagcag
     6421 accggcacgg ttgacggaaa catggtcaag atcaaggcgc gcgttctcaa cgcctccggc
     6481 gagacgaagt ttgccgacgt gaagttcaaa gagacttaca agggcgacaa gtgggacggc
     6541 gcacgccccg atgcgccgct cgaagacacg gctgtctccg tccgccttga gccgggcgag
     6601 gagcgcgagg tcgagatggt ctgggactcg tcgggctacg cgtggttcga cgacgggagg
     6661 ccgcgcctcg tgcagcgcat caaggccgag ttggaagaga gcggcaagaa ggttgacgaa
     6721 ctgacgaaga acctcaaggt ggctccgaag cctctggttc tggttcacgg cctctggagc
     6781 aactggcggg cttgggagtc ttggcagaat atcctgacga cctcgcacag ttacgattgg
     6841 aaggctttcc ccgtcggcga aaagccggag aagggcttga tgaacacggg cggcgagttc
     6901 ctctcttcgg gaccgacgaa cgggattttc gagaactccc agcaactcgg caagtatata
     6961 cgctacgcgc aggaggagag gaacgcgtgg cacgtagaca tcgtggcgca ctcgatggga
     7021 gggcttatct cgcgccatta catacacaac ttcatgccgg ttgactcgcc cgacggacgc
     7081 ccgcaggtcg cgcacctcgt catgctcggc acgccgaaca tgggcagtcc ttgcgccgac
     7141 gtgatgaatt cagctttcga ccttctgggc aagaacgtcg aggccatcag gcagttgaag
     7201 ccttcggtcg tcgccgagtt caaccgtgtg aacgtcaacc gcaagggcgc aaaattctcg
     7261 gtgctggccg ggaaccctct gcccgtcatg tgtaagaccg tggttttgaa cgacggcgtc
     7321 gtccccgtag agtcggcgca ttggctgata aaagacaacg ccctctcgaa gaacctgcac
     7381 acggacttga cgggtacggc tgacttttcg agcttcgtca agccacgtct ggcaatcggt
     7441 ccgaagggca accacaaccc ggagccgccg gagttgccgc gcaactcgaa ccagaccgga
     7501 cgtaactttg acaaccctta cgccgccatg ttcgtcaacg cgtcgtacag gccacagtcg
     7561 tcgagcgtcg ccggggtggg cgatgatctt cgtccggcgt tcgccaaaga ggtaaagctc
     7621 gcgccgaaac agtcgctgga aatcgaactc aacgtcccgg cgggcgcgaa tttcggtgtg
     7681 acgttcatgt ctaacccgaa agtctcggca actctgtttg acgataaagg cgcggtccag
     7741 gggaaaaatc tcgcggactc accagagtcg agggggttgt tccgcacgat tttcgtcgag
     7801 cggggcatca ctgccggaac ctggaagttg aaggttgaaa acagtggaga acaggaagcc
     7861 gtcgccgtca ttgcggcgtg gagcgacgcg gtgaagatgc agagataagc acgtctctat
     7921 gaataagaac gcggggcacc tctcagccgg ggtgccccgc gtcgccgtat tgcagcgctg
     7981 atattttttg taacactacc gtcgtccggc agtcgcaatt gctacgccgc ccgttaagag
     8041 cgcggccttc ggcgcggtga gcttttgtaa agagaattcg tcgagccata tccgaccggt
     8101 atgccagcag ggcggcgcgg cacattgctg ccgctcgatt gcgaacaaga cggcggcggt
     8161 cgtggtcgcg gtcttgaact caacgttgaa ctcctgccat ccggtcgtct ttccccgcaa
     8221 cggtattggg gaagccaact cctcaccact atcggcgtct ctcatgctga gcacaggcgg
     8281 ccagacgctt ttcattccct gtacacgcgc ggcgaagctc aggcggtaac gtgaatcctt
     8341 ctccacgaga accatttgcg agataagggg cgactgcgcc cacccctcgc cgttgaaatc
     8401 gagtcggagg ctgcgcgccc ccgcgcgtgc gccctgtgtg tctgaagaga ctgtaagacc
     8461 tccggggctg gcttcgaccc gccacccgaa tccgaactcc tgcgggttaa tctcaccctc
     8521 aaagccgccg tccgtgatga acccggcctc accgcgccct gcgcccgcgc cgccaacgcg
     8581 cccgcttgac cagaagtcgt aagcctctct gaacttcttc gccgcgatga gttcggacgt
     8641 aatcacacgc cgcgcctctc cggcatcgtg acccgcctga cgaagcaagg ctgccgctgc
     8701 gccgctcgcc gcgccgcgtt tgacgaagaa gcgcgcgagc gcagtgcgcg ccgttgccgt
     8761 gtgcggcgag atggcctgcg ccattgcgtc cgcgtcgccg ccggacgcct cccagagaag
     8821 ttcgagcgcc tgcggcggaa agtccgggtc gcttgcgacg gcgcgagaaa actccgcgaa
     8881 cgcgcgttcc cgctctcccg cacggagcaa cgcgccgccg agtacccagc gcggctcggc
     8941 gtagaaggga gcgagccgca cgccctcctg taaagccgcc atcgcgcccc gcaagtcgcc
     9001 accggcctcg cgcactaaac cgagcctgag ccacagcgag tatagacgcg ggcggagttc
     9061 ggcggcgcgt tgcaactcct cccgcgccgc atcgttcctc ccgctggcga ggagcagttc
     9121 ggcgcgaaaa taacgtgcgg taggatcgtg agggctgaga cgaatggcac gctcggccaa
     9181 cgacagcgaa taatcatgcg cttgccccac agagtaccgg cgagaaagac ccgcacgcgc
     9241 cgtcgcccac atcagcgcgc cgcagcagag cagacagagt gcgacggacg caacacgcac
     9301 tacccgtccg tcgctggccc gctggaagag gtctttaagc gaccggcgga cggctcggtc
     9361 ttgagcatga actgcgcggg cgggctgaac gcgcttgtcg gcgcagacat gtgccgcagc
     9421 gatgacgatg agcgcggtga agaggtgtgc gttggccgtg atgtgcaaac cgaaatcaac
     9481 gaagctgtgc agagcgacgc cgcacaaacc tgtcaacgcg ccgcagcacg ccgcccggcg
     9541 gaaaggatcg ccggagcgga ggcactcgcg cgccctttga ataaatagac ctccgaacag
     9601 agccgccgcg gcgatggcga cgagtccgcc gctggcgagt agttccagat aatcgttgtg
     9661 ggcttgttcc agtaagccta tgccggaggc gtcgaagaat ggggtgatgg cgagccagta
     9721 gccgccgaag cccgtcccgg cgagcgggtg ctccttgatc aactgccacg tcgcccacca
     9781 tatcctgctc cgccgtggga agtcgcgtgt gcccgcccct tgcgcgccga tctccccccg
     9841 cagactctcc atccgctgca tcagtatgtc gccgccaatc cagaccgcag cgaagcccaa
     9901 agcgaccagc agacacgtcg tcgccgtcca gcgcacgatc agtggatgag cgaaccaacg
     9961 ccgcgtcacg gcggtcttct cttcgtcgtg ccttcgccgc cggggagtga gaacacccca
    10021 caggagggct aggaaaacga tctgccccgc agcggcgaag atgccgccgc gagaggtcga
    10081 gagcacaagc gcagcccaga gcagtgcagc cgcagggagg cagaggaaga gtcgaaggcc
    10141 gcgaggcagc gcgcccgcac ccgccgccag cccgagcagc aggccgaggc acatttccaa
    10201 caggagcgcg aagtggttgg cgttgaacgg ggcgtattcg agttgctgcc aaaggtaagg
    10261 tgaggcgaat ggctcgccgt cggagtgcag cgcacgccgc gcgaggccga acagcgcgct
    10321 tgagacgccc gcgacgagca cggcgcagat gagtgctgcg agccgacgcc ggttcgacgt
    10381 gtagcgcagg agcatggccg cgacgagtag gtgagcgagg aagcggaagg cggcgagccg
    10441 cgtctcgtaa gcgtccgcgc tgagcgtccg ccaaacctca acgcccgcca cttcttcccg
    10501 ccccaagggg acggactgca agaagacgaa gaggagcagc ggcacgagtg gcgcgagcag
    10561 cctgtgttcg cgcacgaacc atcccccgcc gagcagccct tccaacgccc acaacgccgc
    10621 gagggcgaac accacgcatt ggaacaggga cacccaccac tctcttacca gcccatactt
    10681 ggcgggtgcc aacacgatca gcgagagcag cgcgtagaag atcacggctg ccagcgtgcg
    10741 agacgcgcgc ggctgcgtgc gagacgcgcg cggctgcgtg cgcggcgtcg ggtcgtctcc
    10801 cgcgacggcg gggtgctgtg ccccggtgtg ggcagtcatc ggagacgact cctcttgcag
    10861 taagagcgtc gccgggagac tgcctgcctg tagcatccgg ctcatagtta aataaggaga
    10921 ggcgagctac gcctccggtt cgaccggcca caccgctata atgatttgag gaacttgtac
    10981 aactgcgcct tttgcaagag gcttaaagac tggaacgcgt tcctgctcgc agtggcctgc
    11041 cccccgtggc gcatgatcgc ctccctgagt gtgaaagact gcaccccgct gttcgacggc
    11101 gcgctggacg atcccccgtc gtgcatgagc cggtcgtgcg ttcgcagtcc ccagagcggc
    11161 ggcgtgcgaa ccatcagccg cgtcgcctcg ccattggttt cgacgatgcc gtcgcccgtc
    11221 cctatgtcgt gcagcaggaa gtcgctgaag gggtgaataa tcttgtcgcc gagcgcggcg
    11281 ggaactacaa aagtgccgcc gttgatcacg gtgcctgcgg gagccgtcgt gatggagcgg
    11341 acgtgacaga ccgaacagct tagctggtcg aagagttgcg agccggggtc ggtcgagtcg
    11401 tcgggcacga gggcgcggtc acgcggcgga accttcgtcg cgcgcatgaa gcgtgtgaac
    11461 gcctccacgt cgtcgccgaa gccctgttcg ccgcccgtgt cttccgcgtc gggcgtgagg
    11521 tcgaaggcca tcacgttgcg gccaagcgag gtctgctcgg tcggcaggag cgggctggtg
    11581 atgcccatct cgaacaagta agccccggcg gagaagctga gcaggctggc gacggagttc
    11641 ttccagccga agcgtccgag acgcgtctgc tgcggcgctt cgagcagcga gacggagata
    11701 gccgtgccgc gcatctccac aggctggttg tcacgtatgg acaggagcgt cgcgtcgtca
    11761 atggcttcga cgaagccgtc gccgaggatg ttgagcgagg agcggaaggt acgcacggtg
    11821 tcgccgtcca agtctctgga gtaggcggga tagttgccgc cggctatgga gatttgcgtc
    11881 tgggtgctgc cgtcggtagc catcgcccag atgcgcgtgg cgttgttgcg ggtgctggcg
    11941 aacgcgatgg acgcgttatc gggcgaccat gtgggattag tgtcgtcggc agggtggttg
    12001 gtcagccgcg tcggcgtgcc gccgctggag ggagctttat aaatctccca gttgccatca
    12061 cggtctgagc cgaagacgat ggacccgtta tcgggtgaga agctgggacg ttcatcgttc
    12121 ccggcaccga acgtaatttg cgtctgccct gtcccgctcg atgacattgt ccaaatcttt
    12181 tcaaacggcg gagtcatagt tggctgcgac cttgtgaagg tgatactgcc ggagttggac
    12241 gaccaggcag gccagctatc gttggcgttc gagttggaaa tattcacctg attggtgccg
    12301 tcggggttca tcaggaagat ttgccagata cccgtgcgac gactgccgaa ggcgatcttc
    12361 gtgccgttcg gcgaccaggc gggcgacatg tcaactcccg tgttggtgag ttggcgcagg
    12421 ttcgtcccgt cgttattcat aacgaagatg ttgatgttgc cgccgacgct gcgctgaaag
    12481 acgatctgcg tgccgtcagg ggagaaggcc gggtagactc ctgtgacggg cgagttgccg
    12541 acaactccgt actggccgcc gtcaaaaccc atgacggaga tcagttgatt gtcgcccatg
    12601 aagacgatgc gcgggccgtc agccactcgc tcctgaatcg aaggatcgac agcgcgcgcc
    12661 tggatcagcg agccgccggg agccgggaag aaggagaggg gagtaagtgg cgcgggaaca
    12721 gtttgacgac tgggggagaa aacgggaggg tttgaagtag tggtgaccgg gttgaggccg
    12781 cgacctgcgc gcagttcgaa gagttggctg atgccgcccg taaccgggtt ctggtggcac
    12841 tcggagcaag cctgcgcgtt gtagacgggg ccgaggccgt taatcctctc ctcgcgcttc
    12901 tcgaaggcgg cgcggtcggc gtcgaaggtc gcctggtcgg tgaagccgtt ggtctggttg
    12961 tcgaaaccgg cgggcgcttc cgtctggccg tgtgcggttc tggtgggggc tgctatcgtg
    13021 gcgattaagg ctgccgcgaa gatcgcgatg atgagcagtt tgaggacttt catttcgctc
    13081 tcctcatttg tcgaaaccac tccgcgcgga attgggggag ggtttacacg ctcgtatgca
    13141 gtgtcggcgc gtgacgcagg tgcgaggatg tacgcgactc gaaagaggcg cagacacggt
    13201 cgtccgcacg cgtttcgaca gacccgcaag tcagggcgga gccgacgaaa accggaaaag
    13261 tcacctgcct cacagtccgg ttgacggcag agcaggctga atttagaaag aagaacggat
    13321 gtgacggtaa ggactacacg ccgcgcccgg ttaagaagat tgccgtgaga ttaaccggag
    13381 agggattgcc ggaggcaaaa ctccctctga ctaaagacga atgcaaagcg attgcgaagt
    13441 tggacttgct gaacgaggcc ggcaaaaggg cttgcgtgag tatccgtttt gccgcccgga
    13501 agaaaacgtt tcggtcaagg cgtcactcta ctcgcaaaga attgcgtcgt caagaaattt
    13561 ttccgcaggc cgctgcgcgc gaaaaatctc ataactctcc gccggaaaag ccgtgtgacg
    13621 ccgcgcctcc gacgcgtcgc gagcgtccgc ccgacgctct tgccagacgt gtttttcgtc
    13681 tgacgggtag agggaaccgt ctgcgcacgc gagtagtcgt cgatggcatc tttcggctcc
    13741 aatgtctgcc ttttagaagt tcgttcttct tgaaaaatat caggtacgtc cgtaccaacc
    13801 ggaacaggcg ggatgagttg tgcgatggtg ctcccggtct agacgggttc aataaagggc
    13861 aaggagaaac tgaagtttga tccgactccg ctctcgctct gcacccagat actcccgccg
    13921 tgcccctcta caattctttt aactattgcc aggccaagcc ccgtactctg ctcgcctccc
    13981 gtgctcttga cactgagatt ttcaaaggga ataaacagcc ggtcaatctc attcgccggg
    14041 atgccctgcc cttcgtccct gacactaacg acgacctctc tttctctctt cgtagcccag
    14101 acctctactt tactgccggg gtgggaaaat ttaatgccgt tactgatgag gttattcaga
    14161 acttgttcga tcttgggcgc gtcgatgagt aacgcgggta ggtcttcccg gtgtgtgaag
    14221 aggatattga tctgcttttg tccggccatt aaacggttca actctacgtt tctttccagc
    14281 agggcggcca agtctgtctc cttcaactcc agttgtaact taccggcctc aatttttgag
    14341 atatacagaa ggtctgtgat caggttcaac atgaagtcgc tgttttttct gatgacggag
    14401 atgaacttgt gttgctcggc agtgagttcc ccgaagtgct tatcgagtaa tatctggcta
    14461 tagccttcta tgacctggag ggggtttcgt aaatcgtgtg cggcgactcc gataaactgg
    14521 ttcttaacct cgttgagcct ttccagttcg atgttccgtt tgatcatttc acgctgggcg
    14581 gtgacaagtt cgttgttgag gcgcgcgagt tcgttgtaaa gctctgtgtc tccgtcgaag
    14641 agttgcgcag gtgcgctgcc cggccatccc tgcgcggtgc tgttcccgga aggactgtct
    14701 acgacgtgta cgaattgctc gtagaccttg atgatgcctg agcgcgtggc ggcgcccgca
    14761 atgaatatgc cgtcgccggt ggcgctcccg ctaaagcgta ttgacctgac ctgctcgccg
    14821 acggcgagat tcaactccca gtcgaatgcc gcccccttct cccgcacgac ttcgaggaac
    14881 gcgcgcccct tctcacggct ctcaaaatcc aatgcggatt caaacggctg cgcggaagct
    14941 atttgccctg cgcccaaccc gtcatagaga acctgctgca cgacgccatt cgggtcgcac
    15001 agcagggcaa cggcggcaag ctctacattt ttactcgtca ttgcgccccc gttgaaggtt
    15061 catagactgg aagccatcgc gaccgcatcc gccgcatccg ccgcgtaggc gtctgcgccc
    15121 acacgcctcc acaactccgg ctcgacattg aacggatacc cgccaaccag aatcttaacc
    15181 gccttcaaat cttccgacgc gcgaacggcg gcgattaaat tttcaaccgc acggatgtga
    15241 aaagtcatgg tcgccgaaac cgcgagcacg tctgccctgc gctccgagag ggcttgaagg
    15301 atggcatt
//
LOCUS       DASWNS010000375        17677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_40723, whole genome shotgun sequence.
ACCESSION   DASWNS010000375 DASWNS010000000
VERSION     DASWNS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17677
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40723"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VGB76_13175"
     CDS             <1..409
                     /locus_tag="VGB76_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099893.1"
                     /translation="ICAEAEHRFAVARSLVENGYEAYVAEDTTQAIERMREDQMDIVL
                     LDPHFDQPEQGAAFIKREVAALRPAARRRLFFVHLSPDARSGDAHMAFVNHANLTINP
                     TEIEEMPYVLERAIRNFNDLYRDFNKALQVVEF"
     gene            661..1626
                     /locus_tag="VGB76_13180"
     CDS             661..1626
                     /locus_tag="VGB76_13180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0099894.1"
                     /translation="MNAVSEQHEHGAHAHQHLSVRAERSRRRLLIVLALTACYMLAEA
                     VGGWWTNSLALMADAGHMLTDVAALVLALMAVWFGARPATPRKTFGYHRLEILAALVN
                     GVALVVMALLIFYQAYERWHTPREVESNLMIVVASGGLLVNLACAWLLHSEHTDDLNV
                     HGAWLHIMGDALGSVGAIIAGVLMSVYGWYQADSLFSAAIALLIIWSSWHLIKESTNV
                     LLEGTPAHINLALVEEAIRTTEGVEGVHDLHVWTITSGREALSAHVVHAPTVVQPELL
                     KVLRVKLHDRFGVDHLTLQMETEDFEDETFHFCHAGTACFSSTRD"
     gene            1696..1938
                     /locus_tag="VGB76_13185"
     CDS             1696..1938
                     /locus_tag="VGB76_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099895.1"
                     /translation="MEINLLLTLDLREQAALQAALVTHGAPDHLVTLALTGACRIQTL
                     QEARRLRRWLVEARKAGGADFASLDVIESALTRFGI"
     gene            2057..3325
                     /gene="larA"
                     /locus_tag="VGB76_13190"
     CDS             2057..3325
                     /gene="larA"
                     /locus_tag="VGB76_13190"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEY0099896.1"
                     /translation="MSSIELGYGRAQLSFVDEEERFQILAPAEETALQPPLSDAQMGA
                     LIDDPFDSQTLEEIISPNESVLIVVSDATRATASGQVVNLLVRRLVQLGVAPYDLRII
                     FATGIHRPVTTEERRELLTPFVLQRIRTLDHNADDQAAHVELGTTERGTPIELNRALT
                     EHDHVILTGAINFHYFAGFTGGRKSICPGLASARTIAATHLHALDFETGARRAGVGAG
                     RLDGNAVHEECERIAAEIAPSFLLNTWTDEAGRAVRLYAGDWRSAHRLACAEYADAHT
                     LPVAEKRPVVVASCGGWPHDLNLIQAHKTLDMAAHACTEGGAIILVAECADGLGRADF
                     LQWFDAENSHALAARLRESYAVNGQTAWSLLTKAERFNVQLVSRLDDADVRRMRLTPA
                     RTIEEALAQVGGDAQGFVMPYGARFMPVVA"
     gene            complement(3426..3725)
                     /locus_tag="VGB76_13195"
     CDS             complement(3426..3725)
                     /locus_tag="VGB76_13195"
                     /inference="COORDINATES: protein motif:HMM:NF041742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGxxGxxG family protein"
                     /protein_id="HEY0099897.1"
                     /translation="MNEGRLAKFLRVGLLSLTLLTVAVPVAAQTSDNANGNTSRPAET
                     RDDRREVRTADREDDKDWGWLGLLGLAGLLGLMPKKKREVHVRETHDVRDAGNRR"
     gene            complement(3800..4918)
                     /locus_tag="VGB76_13200"
     CDS             complement(3800..4918)
                     /locus_tag="VGB76_13200"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0099898.1"
                     /translation="MAKTVIGLFDNRAEAQSVVQELLSENFRREDISVMSKKLEGQDA
                     GDERVEYVEEDGDEQIKDMAKGAGTGAAIGGLAGLLLSLTALAIPGIGPVVAAGPLAA
                     IIAGAGIGATAGGLISGLTRLGVTEEDANYYAEGVRRGGTLISVDAADDRAERAVAIM
                     KRHGAVEIDKRAAEWRNEGWSGFDSNATQNAFDAFSGGGQKFASDAETTAEHQRLPIE
                     PREPRRATTANAASREVAVPVVEEQLQVGKREVEGGGVRLQTQVSETPVEENVQLREE
                     HVNVERRPVDYTFHGTESEAFKESLVEIREAYEELVVKKKARVVEEVVVNKDVEQRTE
                     TVHETLRRTNVNVEPIEGGKARGAGASSAQKNDDLLDI"
     gene            complement(5054..5806)
                     /locus_tag="VGB76_13205"
     CDS             complement(5054..5806)
                     /locus_tag="VGB76_13205"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0099899.1"
                     /translation="MSQTRGTAPREKSAIVTDRDGLRGRIDAYAPQAQGEGGRPEVFV
                     HLDDGRQVLMPVDELVLQNDGSFTVPFSLSDTAHPQRRESVREINPREEAGDETLISS
                     NRTREDGRIVMPVVAETLDVQKRKVETGGVRIKKLVHEREEVVDEPLMREEVQVKRVP
                     INRVVDAPVPVRHVGNTMIISLLEEVLVVEKRLMLKEELHITKGEVETYKPQRITLRS
                     EEAVVERVEDADETQSRTATGTHPPEVRERER"
     gene            complement(5855..6100)
                     /locus_tag="VGB76_13210"
     CDS             complement(5855..6100)
                     /locus_tag="VGB76_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099900.1"
                     /translation="MSEDRLPVTGLKSDGGDAARRDAPSVASGESVQSRPRTATMQRT
                     VGVYERPAQRTRLSLPLLVIVILATLISVVLSVRFLF"
     gene            complement(6300..7235)
                     /locus_tag="VGB76_13215"
     CDS             complement(6300..7235)
                     /locus_tag="VGB76_13215"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0099901.1"
                     /translation="MANSTGTTQKRSQVVTGMFKDRDSAERAYTSLSERGYSKDDINV
                     IMSDETRNKYYPEGSDAADTELGSKALEGAGAGSAIGGTTGAILGAIAAIGTSVALPG
                     LGLLVAGPLAGALVGAGAGGAAGGLIGAMVGSGIPEDRAKEYEEGVKEGGIVMGFNAR
                     SDEDAEHFEREWNTNKGENVYRGETTIPVVEEELQVGTREVERGGVRVESRVEETPVT
                     EEVQLREERVHVERRPVDRPVTDADHAFREGTLEVTERAEEAVVAKTAHVVEEVVVGK
                     QVEEHTETIRDTVRRTDVDVQEVDTNETTRKGRKR"
     gene            7542..8222
                     /locus_tag="VGB76_13220"
     CDS             7542..8222
                     /locus_tag="VGB76_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099902.1"
                     /translation="MKAIIKAFCLALVLSACGAGARAQQQTDASAGATGSSGEPRVAL
                     TEAATAFDAQGRVALAGRLRTSTLTGTQDAPARNVFIAIENRGNAFYNYVSGTATFYG
                     ADGVRCGEGLWKVGTLAVGEAAEVDTPGLRLTCTPTTWRIVAINLVTRSTDAAKPDSL
                     SAPAATDDPAAITTTQNAQPSTTSTAATMPRLEININGRTLPLQIGNPLEIVVGSERV
                     RIIVNPAP"
     gene            8319..9746
                     /locus_tag="VGB76_13225"
     CDS             8319..9746
                     /locus_tag="VGB76_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0099903.1"
                     /translation="MSRHHEGLNGRGRARTAASEEGVLASLREIVGAEHLLVDEARVE
                     PYAADAVKEKFPPEAVLLPQTAAEVAAILKLANERRFPVTARGGGVGYTGGAVPIEGG
                     VVIGTDRMNRIRAIHADDLYVVTEPGVTTYALQQAVEAEGLFYPPDPASYKTSFIGGN
                     IAENAGGIRSAKYGVTKHYVLGLEVVTPTGEIIRTGGLTSKNVVGFDLTGLICGSEGM
                     LGIITEATLRLLPLPEATRTVRATFKTMTEACACVPRFNRARVTPVAIEVLDRNAIAA
                     VETEFAFGFGAEAGALLLVSVDGHAEEVERAARIVEGVVRDGGGFDVMRSETREEEER
                     LWDVRRAISPALKKFGTMKFNEDVVVPRSRVPELVARVEEIGRLHDTFVVNFGHAGDG
                     NIHVNFMCDRDNKDAMRRARAAVRDTFAVSVNLGGTISGEHGIGYVKAPYLDMALGTT
                     TIDAMKRIKRALDPNGILNPGKMFV"
     gene            9770..10150
                     /locus_tag="VGB76_13230"
     CDS             9770..10150
                     /locus_tag="VGB76_13230"
                     /inference="COORDINATES: protein motif:HMM:NF044559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099904.1"
                     /translation="MNDGELQKIVDAWITGMEADRDASERESNRWAISEVIDWKYDGE
                     AELLWRFVLAGYKRELSDKVLACLAAGPLEDLLSAFGAAFIDRVEELARKDPQFNWLL
                     GGVWRLAMTDEVWARVQAARLKAW"
     gene            10162..10740
                     /locus_tag="VGB76_13235"
     CDS             10162..10740
                     /locus_tag="VGB76_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HEY0099905.1"
                     /translation="MEALLIELQRLKRLDRTGWVLRGLAPGAESVAAHSYGVAFTAML
                     LADEVRARGVEVDVERVLRVALLHDLAEARTGDMPRTMALYYGAEARHAAERAAFDDM
                     VSSLGAARSAEYGALHADYEARESLEARLVKAADILDLLAQALAFERAGARELDEFWE
                     GAETRDFNLEGASKDVVSEMLAALVAARGALK"
     gene            10801..11238
                     /locus_tag="VGB76_13240"
     CDS             10801..11238
                     /locus_tag="VGB76_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099906.1"
                     /translation="MFDWLRETVTTYMSGRSFVWLILFSVITFLASIAITILVLIKLP
                     DTYFKASHGREFWVERHPVLRWGGLIVKNLLGAFLVLLGVLMSLPGVPGQGVLTILLG
                     VMLLDFPGKRSLELKLVSRPKVLRTINRIRQRFDRTPLQLDEA"
     gene            11493..12509
                     /locus_tag="VGB76_13245"
     CDS             11493..12509
                     /locus_tag="VGB76_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099907.1"
                     /translation="MLAFILGLVSLAGVSGCSRFYKSKETVPPLLSPLKEAAPGELIE
                     VVNRMAQVHSLRGKIDIQFLDNSFAQCGITEKYRTAEGDVILQRDGKIYLVINGPFSV
                     KIAEMTSDGERFRVAVLQGDDKWRRFLKGTNTANYQRIESGAAGEVDCGKGDQTKTAQ
                     MQQRAASAFSGLRPQHFTDALLLRPVGGGAASNLLYVRSEAFAEDIDTRPRARKGARV
                     VRGYYQLDEIAPQPDGRGRLLRRFWFDRYGEIALARLQTFGEQGELTTDVVYREQKSF
                     GEATKYRLPAAIEITRPQDRYSLRITYQTPDAVKVDQTWPTEVFVLENKSNLPEVDLD
                     ARRQ"
     gene            12626..13729
                     /locus_tag="VGB76_13250"
     CDS             12626..13729
                     /locus_tag="VGB76_13250"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0099908.1"
                     /translation="MESLVANNIRQRPLRALVSMAGVALGVCLVMLFTGLSRGMSNDL
                     QRRSSSVLAEIYFTRPGGMELTTSTASLSTKYADELMKIEGVESAVPVIRYIFQGGRG
                     FGFEQVQGVDWEPYAAMNKLKLVAGSPPVAPDEIIIDETKARNGNLGVGSTLSLFGGK
                     PYRIAGIYTPEAEARVKMSLAAMQDALEAPGKCSYILIKCKEGFDPLAVVQRLEANPT
                     TQGNKIQFTRDIVTNIEKQIPYLSVFLNALVVLAAIVSALVVMLAMYTTITERTREIG
                     MLKALGASRGYIVSVIEREALLISLLGLAAGFAVSFAAGFVINRAYGLLFEYGWKWAM
                     VAALIGVGGGVLGALYPAVRAANLDPVNALSHE"
     gene            13882..14586
                     /locus_tag="VGB76_13255"
     CDS             13882..14586
                     /locus_tag="VGB76_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0099909.1"
                     /translation="MKTILQAENVEKAYRVGSVDVHALRGVSLDLEEGEFLAIMGPSG
                     CGKSTLLHLLGGLLTPTSGRIVIDGEDLTAASDAKRTDIRRRKIGFVFQRFNLFPTLT
                     AEGNLRLAERIHAGGRNGGDGDAERRREVLSLLKLEDKMHHKPLELSGGEQQRVALAR
                     AIVNRPAIILADEPTGNLDSENSAIVLNMFQEMNYRFNQTIIMITHNPEAAAMCRRIV
                     QMRDGQIVAPNPGLQV"
     gene            14644..15030
                     /locus_tag="VGB76_13260"
     CDS             14644..15030
                     /locus_tag="VGB76_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099910.1"
                     /translation="MLLWQPLFRDFDGEAVLRFLQKLFFWSYGRTTWQYDVLCVLILA
                     FIFLTPGKWFENSERVHRERHQNASTTATRLLLVWPENSPANPDTEEVTRRVRSVTNR
                     ADVRVKGMNPVKGAGGQIVAYEVDIE"
     gene            15102..15800
                     /locus_tag="VGB76_13265"
     CDS             15102..15800
                     /locus_tag="VGB76_13265"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY0099911.1"
                     /translation="MKRYLTLGLALVLTAIVAISSTPLKANAQSAGLVSSIFSKMDRN
                     RRDLRSMRASISMEKYNAQLKDSDKQTGTVIYLPGAGQNAYVRIDWQRPQQETLAVAD
                     GKYTLLRPRLKMAYVGEANSNRNKVSSVLGFGLDATKSQLAAAYDTQNVYSETLWGGV
                     QTMHIKLVPKGGARYSYAELWVDDNGMPVQTKVVEKNGDATTVRLMNVQRNAQVSKSE
                     FKVDLGADIKRVRG"
     gene            16041..17057
                     /locus_tag="VGB76_13270"
     CDS             16041..17057
                     /locus_tag="VGB76_13270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0099912.1"
                     /translation="MKRSSFLKLKAREVSFIARGLMSPNHPVLAHVIPMRRCNLACAY
                     CNEYDDSSKPVPTEEMIRRLDKLADLKTSIVTISGGEPMMHPDIYEIVAHIRRRGMIA
                     GLITNGFYLTPEKIRRFNEAGLEYLQISIDNVEPDEISKKSLKLLDKRLQWLAEHSDF
                     HININSVIGGGIKNPEDALVIGRRALELGFSTTVGVIHDGQGALKPLADVEQRVYREL
                     KGLGKQEYARLGWFQDNLAAGRPNDWKCRAGARYLYICEDGLVHYCSQQRGYPGIALA
                     EYTMEDFRREYHAEKPCAPLCTVACSHKVAILDNWRNPQKPFTAKQPTKELSETIRQV
                     LSAE"
     gene            complement(17369..>17677)
                     /locus_tag="VGB76_13275"
     CDS             complement(17369..>17677)
                     /locus_tag="VGB76_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099913.1"
                     /translation="YRAALRLSDDAHTHKLLGVQLAEEGQLEAALTEFRAAERGGAPD
                     ELLVLRIAHTLDALGRPAEARLEYQKFLREKLCTRPTPDALCAGASARAEQIQSGLSD
                     "
BASE COUNT         3285 a         5509 c         5374 g         3509 t
ORIGIN      
        1 catctgcgcc gaggccgaac atcgtttcgc cgtggcgcgc tcgctcgtcg aaaacggtta
       61 cgaagcctac gtggccgaag acacgacgca ggccatcgaa cgcatgcgcg aagaccagat
      121 ggatattgtc ctgctcgacc cgcacttcga ccaaccggaa cagggcgcgg ccttcatcaa
      181 acgcgaggtc gccgcgcttc gtcccgccgc gcggcgtcgc ctcttcttcg tacacctgtc
      241 gcccgacgcg cgcagcggcg acgcgcacat ggccttcgtc aatcacgcga acctgacgat
      301 caatccgacc gagatcgaag agatgcccta cgttctggaa cgcgccatcc gcaacttcaa
      361 cgatctctac agagacttca acaaggctct gcaagtcgtc gaattttaag gccgggcgcg
      421 gcgcgccacg ttcgcgccgc gtcgctccat aaccgttatt gaccattgcc ggatgcgctt
      481 gtaatcttac aggcggcatc cggcttctct tttttatttc gcaactttag cggaagatga
      541 aaacattttc tgcaccactg agagcggaca ttcaaactcc ttgcgagaga tgggaagact
      601 atcgcggtcg cggaaatatt ggttattcga attcaactgt aagggcagag tattgaagag
      661 atgaacgcgg tttccgaaca gcacgaacac ggcgcgcacg cgcaccagca tctatccgta
      721 cgcgccgagc gcagccgtcg ccgcctgctg atcgtgctcg cgctgacggc ctgctacatg
      781 ctggccgaag cggtcggcgg ttggtggact aactcgctcg cgctgatggc cgacgccggg
      841 cacatgctga cggacgtagc cgcgctcgtc ctcgcgctga tggccgtctg gttcggggcg
      901 cgtccggcca cgccgcgcaa gaccttcggc tatcaccgtc tggaaattct cgcggctctc
      961 gtgaacggcg tcgcgctcgt cgtgatggcg ttgctcatct tctatcaggc ttacgagcgt
     1021 tggcatacgc cgcgcgaagt tgaaagcaac ctgatgatcg tcgtcgcgtc gggcggcctc
     1081 ctcgtcaatc tcgcctgcgc gtggctgttg cacagcgaac acacggacga cctgaacgtg
     1141 catggcgcgt ggctgcacat catgggcgac gcgctcggct ctgtgggcgc gatcatcgcg
     1201 ggcgtgttga tgtccgtcta cggttggtat caggcggatt cgctcttcag cgcggcgatt
     1261 gcgctcctga tcatctggag ttcgtggcat ctcatcaagg aatcgaccaa cgtgctgctt
     1321 gaaggcacgc ccgcgcacat caatctcgcc ttggtcgaag aggctatccg cacgaccgaa
     1381 ggcgtcgaag gcgtccacga cctgcacgtc tggacgatca cttcggggcg cgaagccctg
     1441 agcgcgcacg tcgtccacgc gccgacggtc gtgcagcccg aactcctcaa agtattgcgc
     1501 gtcaaactcc acgaccgctt cggcgtcgat cacctgacac tgcaaatgga gacggaagat
     1561 ttcgaggacg aaaccttcca cttctgccac gcgggcacgg cctgtttctc ttcgacgaga
     1621 gattagaggc cggaggttag aggtctgtaa aaagcggcct gacactaacc tccagcctcc
     1681 ggcctctgca cttttatgga aatcaatctt ctgcttacac tcgatttgcg ggagcaggcg
     1741 gcgttgcagg ccgcgcttgt gacgcatggc gcgccggacc atctggtgac gctcgcctta
     1801 acgggcgcgt gccgcatcca gacgcttcag gaggcgcgtc gcctgcgccg ctggctcgtc
     1861 gaagcgcgca aggcgggcgg cgcggatttc gcctcgctcg acgtgatcga aagcgcgctc
     1921 acgcgtttcg gaatctgaca aataggggaa aggggaaggg gcggagagga agggaagcga
     1981 caggcgtgaa agcggagcgg gcggttgtct gttctccctt ccgccttagc tcttcccctt
     2041 tccccttttg aattttatgt cgagtatcga actgggttac gggcgcgctc aactctcttt
     2101 cgttgacgaa gaggaacgct ttcaaattct cgccccggcg gaagagactg cgttacagcc
     2161 gccgctttcc gatgcgcaga tgggcgcgtt gattgatgat ccgttcgact cgcagacgct
     2221 cgaagagatc atctccccga atgaaagcgt gctcatcgtc gtctcggatg cgacgcgtgc
     2281 gacggcgagc gggcaagtcg tcaacctgct cgtccgtcgt ctcgtccaac tcggcgtcgc
     2341 gccctacgac ctgcgtatca tcttcgccac gggcatccat cgtcccgtca ccacggaaga
     2401 gagacgcgaa ctgctcacgc ccttcgtcct ccaacgcata cgcacgctcg atcacaacgc
     2461 cgacgatcag gccgcgcacg tcgaactcgg cacgaccgag cgcggcacgc ccatcgaact
     2521 caaccgcgcg ctcaccgaac acgaccatgt gatcctgacg ggcgcgatca acttccacta
     2581 cttcgcaggc ttcacgggcg ggcgcaaaag catctgcccc ggcctcgcct ccgcgcgcac
     2641 catcgccgcg acacacctgc acgcactcga tttcgagacg ggcgcgcgcc gcgcaggggt
     2701 cggcgcggga cggctcgacg gcaacgccgt gcacgaagaa tgtgaacgca tcgcggcaga
     2761 gatcgcgccc tcgtttctcc tgaacacgtg gactgacgaa gcggggcgcg cggtgcgtct
     2821 ctacgcgggc gactggcgtt cggcgcatcg tctcgcctgc gccgaatacg cagacgcgca
     2881 cacgctgccc gtggcggaga agcgtcctgt ggtcgtggca agttgtggcg gttggccgca
     2941 cgatctcaat ttgattcaag cgcacaagac gctcgacatg gccgcgcacg cctgcaccga
     3001 aggcggcgcg atcatcctcg tggcagaatg cgccgacgga ttagggcgcg cggatttcct
     3061 gcaatggttc gacgccgaaa actcacacgc gctcgccgca cgcctgcgtg agtcttacgc
     3121 cgtcaacggg cagaccgcgt ggtcgctcct gaccaaggcc gaacgcttca atgtgcaact
     3181 cgtctcccgt ctcgacgatg cggacgtgcg acgcatgcgc ctgactccgg cgcgcacgat
     3241 tgaagaggcg ctcgcgcagg tcggcggcga cgcgcagggc ttcgtgatgc cctacggtgc
     3301 gcggtttatg cccgtcgtcg cctgagcaaa agttaagagt aaaaagagag agggcatcgg
     3361 gaggcgactc ccgatgccct cttcgttcat ggtcgcagcc gtgatgccgc gtgccggggt
     3421 gaaaactacc tgcggtttcc cgcgtcgcgc acgtcgtgcg tctcgcggac gtgaacttcg
     3481 cgcttcttct tcggcatcag gccgagcagt ccggccagac cgagcagtcc gagccagccc
     3541 cagtctttat cgtcttcacg gtcggcggtc ctgacttccc tgcggtcatc tctcgtctcg
     3601 gcagggcggc tcgtgttccc gttcgcgttg tcgctggttt gagcggccac cggcacggcc
     3661 accgtgagca gagtcaggct gagcaggccg actctgagaa acttcgccaa tcttccttcg
     3721 ttcattacct aaaatcctcc ttgtgatttg ccaagcagcc gaagctgcga aaatttccag
     3781 acagacatct ccccgcgcgt tagatgtcga gcagatcgtc gttcttctgc gccgacgacg
     3841 cgcccgcgcc gcgcgccttg cctccttcta tcggctcgac gttcacgttc gtgcgacgca
     3901 aagtttcgtg gactgtctcg gtgcgttgtt cgacatcctt gttaacgacg acctcttcca
     3961 ccacgcgcgc cttcttcttc acgaccaact cttcgtaagc ctctctgatc tcgaccagcg
     4021 actccttgaa agcctccgat tccgtgccgt ggaaagtgta atcaaccggg cgacgctcga
     4081 cgttgacgtg ctcttcgcgc agttggacat tctcctcgac gggcgtttcc gacacctgcg
     4141 tctgcaagcg cacgccgccg ccttcgactt cgcgcttgcc cacctgcaac tgctcctcca
     4201 cgaccgggac ggccacttcg cgcgaagcgg cgttggccgt cgtggcgcgg cgcggttcgc
     4261 gcggctcgat gggcaggcgt tggtgttccg ccgtcgtttc cgcatcggac gcgaacttct
     4321 ggccgccgcc tgagaaggca tcgaaagcgt tctgcgtcgc gtttgaatcg aagccgctcc
     4381 atccttcgtt gcgccactcg gcggcgcgct tatcgatctc caccgcgccg tgccgcttca
     4441 tgatcgccac ggctctctcg gctctgtcgt ccgccgcgtc cacgctgatc agcgtgccgc
     4501 cgcgacgcac gccttcggcg tagtagttgg catcctcttc cgtcacgccg aggcgcgtga
     4561 ggccgctgat caacccgccc gccgtcgcgc cgatgcctgc gcccgcaatg atggcggcga
     4621 gcggaccagc cgccaccacc ggcccaatgc ccggaatcgc aagcgcggtg agactcagga
     4681 gcagaccggc cagcccgccg atggccgcgc ccgtgcccgc gcctttcgcc atgtccttga
     4741 tctgctcgtc gccgtcttct tcgacgtact cgacacgctc gtcgcccgcg tcctgccctt
     4801 cgagcttctt cgacatgacg ctgatgtctt cgcgccggaa attttcgctc aacagttcct
     4861 gcacaacgct ctgtgcctcg gcgcgattat caaacaatcc gattacagtt ttcgccattg
     4921 tttccccttt ctgtttctat ccggcacagc cacgcgcgcg tgctgtgtga aattatgaac
     4981 gctgccaaaa acttccgcgc cttctagcgt cgcgctaaac gctcgaacgc cgacgcccat
     5041 cacattaacg acattaacgc tcacgttctc tgacttccgg cgggtgcgtc cccgtcgccg
     5101 tgcgcgactg tgtctcgtct gcgtcctcga cgcgctcgac gaccgcctct tcgctgcgca
     5161 gcgtgatgcg ctgcggttta taagtttcaa cttcgccctt cgtgatgtgc agttcctctt
     5221 tcagcatgag ccgcttctcg acgacgagca cttcttccag cagcgagatg atcatcgtgt
     5281 tgcccacgtg gcggacgggc acgggcgcat caaccacacg attgatcggc acacgcttga
     5341 cctgcacctc ttcgcgcatc agcggttcgt ccaccacttc ctcgcgttcg tgtacgagct
     5401 tcttgatgcg gacgccgccc gtctccacct tgcgcttctg cacgtcgagc gtctcggcga
     5461 cgacgggcat cacgatgcgc ccgtcttcgc gcgtgcggtt tgaagagatg agcgtctcat
     5521 cgcccgcctc ttcgcgcggg ttaatctcgc gcacgctttc gcgtcgttgc gggtgcgctg
     5581 tatccgacag gctgaagggc actgtgaaac tgccgtcgtt ctgtaagacc aactcgtcaa
     5641 cgggcattaa gacctgccgc ccgtcgtcga gatggacgaa cacttcggga cgaccgcctt
     5701 ctccctgcgc ttgcggcgcg taagcgtcta tgcgcccacg caagccgtcg cggtcggtga
     5761 cgattgcgct cttttctctc ggtgctgtgc ctctcgtttg gctcatcgtt cttcctttct
     5821 ctttcgcgtg ccggaactcc ggcaggctac ctctttaaaa caagaatcgg acggagagta
     5881 cgacggagat cagcgtggct aagatcacta tgacgagcag cgggagcgac aatctcgtgc
     5941 gctgtgccgg gcgttcgtag acgcccactg tcctttgcat cgtcgccgtt cgtggccgcg
     6001 actgcacgga ttcaccggat gcgacggatg gcgcgtcgcg ccgcgctgcg tcgcccccgt
     6061 ctgatttgag tccggtcacc ggaagtctgt cttcgctcat caagccgccc ttcgtcctgc
     6121 ccgctccgtg cttaagaacc gtgccccgcg cgtcgcgctg aaaatggagg gcgcacgtta
     6181 tcgagtcgtg atgtgtgatg ccggacgacg cccgccctcg tcgggtaggg gggcgtcgtc
     6241 agacttagcg cgcgccttca agccttgcga cctccggcgc gcacactccc tcgatcttct
     6301 taccgtttgc gacccttgcg ggtcgtctcg ttcgtgtcga cctcctgcac gtcaacgtcc
     6361 gtgcgccgca ccgtgtcgcg gatggtttcc gtatgctcct cgacctgctt gccgacgacc
     6421 acttcttcga cgacgtgcgc cgtcttggcg acgacagcct cttcggcacg ctccgtcact
     6481 tcgagcgtcc cttcgcggaa agcatggtcg gcatccgtca ccggacgatc aaccgggcgg
     6541 cgttcgacat gcacgcgctc ctcgcgcagt tgcacctctt ccgtcacggg cgtctcctcc
     6601 acgcggctct cgacgcgcac gccgccgcgt tcgacttcgc gtgtgcccac ctgcaactct
     6661 tcctcaacca cagggatcgt cgtctcgccg cgatagacgt tctcgccctt gttcgtattc
     6721 cactcacgct cgaagtgttc ggcatcttca tcggaacgcg cgttgaagcc catcacgatg
     6781 ccgccctctt tcacgccctc ctcgtactct ttcgcgcggt cttcgggaat ccctgagccg
     6841 accatcgcgc cgatgagtcc gcccgccgcg ccgcctgcgc ccgcgccgac gagtgcgccc
     6901 gcgagcggcc cggccacgag caggccgagt cccggcaggg cgacggaagt gccgatggct
     6961 gcaatcgctc cgaggatcgc gcccgtcgtg ccgccgatgg ccgaacctgc gcccgcgcct
     7021 tcgagcgcct tgctgccgag ttccgtgtcg gcggcgtcac tgccttcagg ataatattta
     7081 ttgcgggttt cgtccgacat gatgacgttg atgtcgtctt tactgtagcc gcgttcggag
     7141 agtgaggtgt aggcgcgctc cgcgctgtcc ctgtccttaa acatgcccgt cacaacctgc
     7201 gagcgtttct gtgtcgtacc tgtcgagtta gccatcaatt aatctccatt ctgcacgtat
     7261 gatttttcaa ctcacaacac ggggaaaact aagaaacgaa ctccggtcaa ttccgtgttg
     7321 tgtatgattt cgcgtcaagg acgcaattcc gaatgcctcc ggtgcaagga gcgtaccgaa
     7381 agatgagcgg aaggcgattt gacgcccgcg tcgccgtaag gggcgggatt cgaggcgtcg
     7441 ctgcgccatc attcgtgtac aatcgtcgcc gcgtctgccg ttggcttcgc gttcgtccgg
     7501 cgcatctgga aaactggctg tagaggaagg ggagtgcaaa agtgaaagca atcatcaaag
     7561 cgttttgtct cgcgctcgtg ttgtcggcgt gtggtgccgg agcgagggcg cagcagcaga
     7621 cggatgcgag cgcgggagcg acggggagca gcggcgagcc gcgcgtcgct ttgacggagg
     7681 cggcgacggc tttcgatgcg caagggcgcg tcgcgctcgc cggacgcctg cgcacgagca
     7741 cgctcacggg cacgcaggat gcccccgcac gcaacgtctt catcgccatc gaaaatcgcg
     7801 gcaacgcttt ttataactac gtctccggca ccgcgacctt ttacggcgcg gacggcgtgc
     7861 gctgcggcga aggcttatgg aaagtcggga cgctcgcggt cggggaagcg gcggaagttg
     7921 acacgccggg actccgcctg acctgtacgc cgacgacatg gcgcatcgtc gcgatcaatc
     7981 tggtgacgcg ttcgactgac gcagcgaagc cggacagcct gagcgcgccc gccgcgacgg
     8041 acgatcccgc cgcgataacg acgacgcaaa atgcgcagcc ctctacaacc tccactgcgg
     8101 cgacgatgcc gcgtcttgag atcaacatca acggcaggac gctcccgctg caaatcggca
     8161 acccgttgga gatcgtcgtc ggcagcgagc gcgtccggat catcgtcaac cccgcgccct
     8221 gaagagaggc gcaagagagg cgcgtgctac gcgtgtcgaa cgaagcgcgc gcgttatgaa
     8281 gtcgtagctt taacgggcgg cgcgtttcta cgttgcttat gagtcggcat cacgaagggt
     8341 taaacggcag agggcgtgcg cgcacggcgg cgtccgaaga gggcgtgctc gcctccctgc
     8401 gcgagatcgt gggcgcagag catctgctcg tggacgaggc gcgcgtcgag ccatacgcgg
     8461 cagacgcggt gaaagagaag tttccgcccg aagcggtgct gctgccgcag acggcggcag
     8521 aggtcgcagc gattctcaag ctcgccaacg agcgacgctt ccccgtcacg gcgcgcggcg
     8581 gcggcgtcgg ctacacgggc ggcgcagtgc ccatagaggg cggcgtcgtc atcggcacgg
     8641 atcgcatgaa ccgcatccgc gccatccacg cggacgatct ctacgtcgtc accgagccgg
     8701 gcgtgacgac ctacgcgttg cagcaggcgg tcgaagccga agggctgttc tacccgcccg
     8761 atcctgcctc ctacaagacc tctttcatcg gcggcaacat cgcggagaac gcgggcggca
     8821 tccgctcggc caagtacggc gtgaccaaac attacgtgct cggcctcgaa gtggtgacgc
     8881 cgacgggcga gatcattcgc accggcggcc tgacgagtaa gaacgtcgtc ggcttcgatc
     8941 tcaccggcct catctgcggc tccgaaggca tgctcggcat catcaccgaa gcgacgctca
     9001 gactgttgcc cctgcccgaa gcgacgcgca ccgtgcgcgc caccttcaag acgatgacgg
     9061 aggcttgcgc ctgcgtgccg cgtttcaacc gcgcgcgcgt gacgcccgtg gcgattgagg
     9121 tgcttgacag gaatgctatc gcggcggttg agacggagtt tgcgttcggc ttcggcgcgg
     9181 aagcgggcgc gctcctgctc gtctcggttg acgggcacgc ggaggaagtc gagcgcgcgg
     9241 cgcggatcgt ggaaggagtc gtgcgcgatg ggggcggctt cgacgtgatg cgttcggaga
     9301 cgcgcgagga agaggaacgc ctgtgggacg tgcgccgcgc catctcgccc gcgctcaaga
     9361 aattcggcac gatgaagttt aacgaagatg tggtcgtgcc gcgctcgcgc gtgcccgaac
     9421 tcgtcgcgcg cgtcgaagag atcgggcggt tgcacgacac tttcgtcgtc aacttcggcc
     9481 acgcgggcga cggcaatatc cacgtcaact tcatgtgcga ccgcgacaac aaggatgcga
     9541 tgcgacgcgc ccgcgccgcc gtgcgcgaca ccttcgccgt ctccgtaaat ttaggcggca
     9601 cgatcagcgg cgagcacggc atcggctacg tcaaagcccc ttatctcgac atggctctcg
     9661 gcacgaccac gattgacgcg atgaagcgca tcaaacgcgc gctcgacccg aacggaattt
     9721 tgaatccggg caagatgttc gtctgagagg gttcttaaga agcactgata tgaacgacgg
     9781 cgagttgcag aagattgttg acgcctggat cacgggcatg gaggccgata gagatgcgtc
     9841 tgaacgtgag agtaaccgct gggcgatttc ggaagttata gactggaagt atgacggtga
     9901 ggcggaactg ctctggcggt tcgtactcgc tggctacaag cgcgagcttt ccgacaaagt
     9961 gctcgcgtgt ttagccgccg gcccgctcga agacctgctc tcagctttcg gcgcggcgtt
    10021 catcgatcga gttgaagagc tggcgaggaa agacccgcag tttaactggt tgttgggagg
    10081 cgtgtggcgg ctcgcgatga cggatgaggt gtgggctcgg gtgcaggccg cgcgactcaa
    10141 agcgtggtag ccgaagtttc tatggaagct ctgttgattg aactccaacg tttgaagcgg
    10201 ctcgaccgga ccggctgggt gttgcgcgga cttgcgccgg gggcggagtc ggtggcggcg
    10261 cacagttacg gcgtggcgtt cacagcgatg ctgttggcgg atgaggtgcg tgcgcgcggc
    10321 gtcgaagttg atgtcgagcg cgtgttgcgc gtcgcgctgt tgcatgatct ggccgaggcg
    10381 cgcacgggcg acatgccgcg cacgatggct ctctattacg gcgcggaggc gcggcacgcg
    10441 gcggagcgcg cagccttcga cgacatggtg agcagcttag gcgcagcgcg cagcgcggag
    10501 tacggggcgt tgcacgcgga ttacgaggcg cgcgagagtc tggaggcgcg gctcgtgaag
    10561 gcggctgaca tcctcgactt gctggcgcag gcgctcgcct tcgagcgcgc gggcgcgcgt
    10621 gagctggacg aattctggga gggggctgag acgcgcgact tcaatttgga aggcgcgtca
    10681 aaagatgtcg tctccgaaat gctcgccgca ctcgtcgcgg cgcgcggcgc gttgaagtga
    10741 agcaacgtgt gaagcgtctg aggtgaagag agacgcaggg cagagttgca tggaggaaaa
    10801 atgttcgact ggctaagaga gacggtgacg acttacatga gcgggcgcag cttcgtgtgg
    10861 ctcatcctct tctccgttat cacttttctg gcgagcatcg ccatcacgat ccttgtgctc
    10921 atcaaattac cggacactta tttcaaggct tcgcacgggc gcgagttttg ggtcgaacgc
    10981 catccggtct tgcgttgggg cggcctcatc gtgaagaatt tactgggcgc gtttctcgtc
    11041 ctgttgggcg ttttgatgtc tctgccgggt gtgccggggc agggcgtgtt gacgattctg
    11101 ctcggcgtga tgttgctgga ttttccgggc aagcgcagcc tggaactcaa actcgtgagc
    11161 cgtcccaaag tgctgcgcac gatcaaccgc atccgccagc ggttcgaccg gacgccgctg
    11221 caactcgatg aggcataagg cagggcagag gggaagcggt acggggcgag ggagaaacaa
    11281 aagccgcggg acgccgagcg tcgtcttccc ctttcccctt ttccctgcgc cgcgcgggtg
    11341 cgcgcgttga caaggacgga gtgctttgtt agcttcgcct attaactttt taagagaatt
    11401 ttgccggatc ggatgcttcc cggcgtcggc gtctgacact tttaatgaac ggttcaaact
    11461 cattccggac aggctcgcgg cgtaaggcgc gcatgctcgc gttcatcctg gggctggtgt
    11521 cgctcgcggg cgtgagcggc tgctcaagat tttataagag caaagagacg gtgccgccgc
    11581 tgctctcgcc gctcaaagag gccgcgcccg gcgaactcat cgaagtggtg aatcgcatgg
    11641 cgcaggtaca ctctctgcgc ggcaagattg acatacagtt cttagataat tcgtttgcgc
    11701 agtgcggcat caccgaaaag tatcgcacgg cggaaggcga cgtgatcctg caacgcgacg
    11761 gcaagattta tctcgtcatc aacgggccgt tcagcgtcaa gatcgccgag atgacttccg
    11821 acggcgagcg tttccgcgtg gccgtcttgc agggcgacga caagtggcgg cgtttcctga
    11881 aagggacgaa caccgcgaac tatcagagaa tcgagagcgg cgcggcgggg gaagttgact
    11941 gcggcaaagg cgaccagacg aagacggcgc agatgcagca acgcgcggcc tccgccttct
    12001 ccggcctgcg cccgcagcat ttcacagacg cgctgctttt gcgtcccgtc ggtggcgggg
    12061 cggccagtaa tctgctctac gtgcgcagcg aagcgttcgc cgaagacata gatacccgac
    12121 cgagagccag gaaaggcgcg cgcgtcgtgc gcggttatta ccagttggac gagatcgcgc
    12181 cgcagcccga cggacgcgga cgcctgctgc gccgcttctg gttcgaccgc tatggcgaga
    12241 tcgctctggc gcgcctgcaa accttcggcg agcagggcga gttgacgacc gacgtggtct
    12301 atcgagagca aaagagtttc ggcgaggcca cgaagtacag gctgcccgcc gccatcgaaa
    12361 tcacgcgccc gcaggatcgc tactcgctgc gaatcactta tcaaacgcct gacgccgtca
    12421 aagtagatca gacgtggccg accgaggtgt tcgtgcttga aaacaagtcg aacctgccgg
    12481 aagtcgatct ggatgccaga cggcagtgac gagagcagcg acgagagcag tgacgagtga
    12541 caagttaaaa caaacaactt gtcctgcttt cttcgtggct tctctttaac tcgtcacttg
    12601 tcgcctgtca cttgtcgctt taattatgga gagccttgtc gccaataaca ttcgccagcg
    12661 ccctctgcgt gcgctcgtca gcatggctgg cgtggcgctc ggcgtgtgtc tggtgatgct
    12721 attcacgggg ctgtcgcggg gcatgtcgaa cgatttgcag cggcgttcgt cgagcgtgct
    12781 cgcggagatt tacttcaccc gaccgggcgg catggagttg acgacttcga cggcgagttt
    12841 gagcacgaag tacgcagacg agttgatgaa gatcgagggc gtggaatccg ccgtccccgt
    12901 catccgctac atctttcagg gcgggcgcgg cttcggcttc gagcaggtgc agggcgttga
    12961 ctgggagcct tacgccgcga tgaacaaatt gaaactcgtc gcgggaagcc cccccgtagc
    13021 ccctgacgaa atcatcattg acgagacgaa agcgcgcaac ggcaatctcg gcgtcgggag
    13081 cacgctcagc ctcttcggcg ggaagcctta tcgcatcgca ggcatctaca cgcccgaagc
    13141 ggaggcgcgc gtgaaaatgt cgctcgcggc gatgcaggac gcgctcgaag cgccgggcaa
    13201 gtgttcctac attctcatca aatgcaaaga gggttttgac ccgctcgcgg ttgttcagcg
    13261 cctcgaagcc aacccgacga cgcagggcaa caagatacag tttacgcgcg acatcgtaac
    13321 gaacattgaa aagcagattc cctatctcag cgttttcttg aacgccctgg tggtcttggc
    13381 ggccatcgtc agcgcgctcg tcgtgatgct cgccatgtac acgacgatca cggaacggac
    13441 gcgcgagatc gggatgctca aggcgctcgg cgcgtcgcgc ggttatatcg tctcggtgat
    13501 tgaacgggag gcgttgctga ttagtctgct cggcctcgcc gcaggcttcg ccgtctcgtt
    13561 tgcggcgggc tttgtgatca accgcgctta cggcctactc ttcgaatacg gttggaagtg
    13621 ggcgatggtc gccgcactga ttggcgtcgg cggcggcgtg ctcggcgcgc tctatccggc
    13681 tgtgcgcgcg gcgaacctcg atccggtcaa cgctctatcg cacgagtgac gataaagcag
    13741 tgacaggtga caagtgacgt gtgacgagat cagagacaag tttgaagacc ggactgctgc
    13801 tttaacttgt cacctgtcac ttgtcagtcg tcattgtctt atctatctcg tcacctgtca
    13861 ctcgtcactt gtcactgtct tatgaagact atcctgcaag ctgaaaatgt cgagaaagcc
    13921 taccgcgtgg gcagcgtgga cgtgcacgcc ttgcggggcg tctctctgga tttggaggag
    13981 ggcgagtttc tggcgatcat ggggccttcg ggttgcggca agtcaacgct gctgcatctc
    14041 ttaggcggcc tgctcacgcc cacgagcggg cgcatcgtca ttgacggcga agatttaacc
    14101 gccgcctccg acgccaaacg cacagacatc cgccgccgca agatcgggtt cgtcttccaa
    14161 cgcttcaacc tcttcccgac gctcaccgcc gagggcaatc tgcgactcgc cgaacgaatt
    14221 cacgccggag gccgcaacgg cggcgacggc gacgccgagc ggcggcgcga ggtcttgagc
    14281 ctcttgaaac tcgaagacaa aatgcaccac aagccgctcg aactctccgg cggcgaacag
    14341 cagcgcgtcg cgctcgcacg cgccatcgtc aaccgtccgg ccatcatcct cgccgacgag
    14401 ccgaccggaa atctcgactc cgaaaactcc gctatcgtcc tgaacatgtt tcaggagatg
    14461 aactaccgct tcaatcaaac catcatcatg atcacgcaca atccggaggc tgccgccatg
    14521 tgtcgccgca tcgtgcaaat gcgcgacgga caaattgttg cgccaaaccc cggtttgcag
    14581 gtataatgtc cggcggtagc aggtgacacg gacaagggcg tgtgcttttg tctttgccac
    14641 ctgttgcttt tatggcaacc cctttttcgc gattttgacg gtgaggcggt tttgaggttc
    14701 ctccaaaaac tctttttctg gtcgtacggg cgcacaacat ggcaatatga cgtgctatgc
    14761 gtcctcatcc tcgcatttat ctttttaacg cccggaaaat ggtttgaaaa cagcgaacgc
    14821 gttcaccgcg aacggcatca aaatgcttct acgacagcga cgcgtttgtt gcttgtctgg
    14881 ccggaaaatt ctccggcaaa tcccgacaca gaagaggtta cgaggcgcgt gcggagtgtc
    14941 acgaatcggg ctgatgtgcg ggtgaagggc atgaatcccg tgaagggtgc gggcggccag
    15001 atcgtcgcct atgaggttga catagagtag gctttcttta taatcgctcg gatttttagc
    15061 cgggcgaaag tttagttccc cttatttttg aaggagttag catgaagaga tacttgacgc
    15121 ttggcctcgc attagtttta acagccattg tcgcaatttc gtccaccccc ctgaaagcca
    15181 acgcgcagag cgcgggtctg gtcagttcga tcttctccaa gatggatcgc aatcgtcgcg
    15241 acctgcgcag catgcgcgcg agcatcagca tggagaaata caacgcgcaa ctcaaagaca
    15301 gtgacaagca gacgggcact gtgatctatc tgccgggtgc ggggcagaac gcttacgtgc
    15361 gcattgactg gcagcgtccg cagcaggaga cgctcgcggt tgccgacggc aagtacacgc
    15421 tgcttcgtcc gcgcctcaag atggcctacg tcggagaagc caattcgaac cgcaacaaag
    15481 tgtcgagcgt gctcggcttc ggtttggacg ccaccaagtc gcaactggcg gcggcttacg
    15541 acacgcagaa cgtctacagc gaaacgctct ggggcggcgt gcagaccatg cacatcaaac
    15601 tcgtgcccaa gggcggcgcg cgctacagct acgcggaact ctgggtggac gacaacggca
    15661 tgcccgtcca gaccaaagtg gtcgagaaga acggcgacgc cacgaccgtg cgcctcatga
    15721 acgtgcagcg caacgcgcaa gtctcgaaga gcgagttcaa agtcgatctc ggcgcggaca
    15781 tcaagagagt tcgcggctaa gtttcagaca ctaatccagc caaagggttc tggctcatag
    15841 aagaccggaa ccctttgctg tttccggcgc gcgacgcctt tgccggaagc gtctgcggca
    15901 gccggatcgc cccggtcttc ttgcgtccgg tagatagatt tcgtatcttc ttccacaact
    15961 ccggaaagga aggcgagggg cgaaggcagc gatagtatta tccacacggg cgtaaacacg
    16021 cacttttaac cgacggccag atgaagagaa gcagtttcct taaactcaaa gcgcgcgaag
    16081 tgagcttcat tgcgcgcggg cttatgtcgc caaaccatcc ggtgctcgcg catgtcatcc
    16141 ccatgcgccg gtgcaatctc gcctgcgcct actgcaacga gtacgacgac agctcaaagc
    16201 ctgtgcccac cgaagagatg attcgccgtc tcgacaaact cgccgacctg aaaacttcca
    16261 tcgtgaccat ctccggcggc gagccgatga tgcacccgga catttacgag atcgtcgcgc
    16321 acatccgccg tcgcggcatg atcgccggac tcatcaccaa cggtttttat ctcacgcccg
    16381 aaaagatcag acgcttcaac gaggcgggtc tggaatatct acagatcagc attgataacg
    16441 tcgagccgga cgaaatctcg aagaagagtc tgaagttgtt agacaagcgt ttgcagtggc
    16501 tcgcggagca ctcggatttt cacatcaaca tcaattccgt catcggcggc ggcatcaaga
    16561 accccgagga tgcgctcgtc atcgggcgac gcgcgctcga actcggtttc tccaccaccg
    16621 tcggcgtcat ccacgacggg cagggcgcgc tcaagcctct ggccgatgtg gagcagcgcg
    16681 tctatcgtga actcaaggga ctcggcaaac aggagtacgc gcggctcggc tggtttcaag
    16741 acaatctcgc cgccgggcgt ccgaacgatt ggaagtgccg cgcgggagcg cgctatctct
    16801 acatctgcga agacggcctc gtacactatt gctcgcagca gcgcggctat cccggcatcg
    16861 cgctcgccga gtacacgatg gaagatttcc gccgcgagta tcacgcggag aaaccctgtg
    16921 cgccgctctg caccgtcgcc tgctcgcaca aagtcgccat cctcgacaac tggcgcaacc
    16981 cgcaaaagcc tttcaccgcc aagcagccga ctaaagagtt gagcgagacg atccggcagg
    17041 tcttgagcgc agagtagcag gcgcgaaaac ggctccccgt accctcttaa aacttgatcc
    17101 accttgacgc tcactctgac gctcacgcag ggagcaacgc tcgttaattc gctgccgggt
    17161 cgcgcgccgc atcaacgctc aggcgcgtct ctctttcttt cgcgtctcta cgttctttcg
    17221 cgtctctctt tctctctaca actctccgcg tcgtccggcg cgacagccgc gaaggatatt
    17281 tttcaccccc gctgcccctt ccgcttcgct tcgacgaaag ctctgcgcgc tcgcgccccg
    17341 cacacgcttg cgaagaagac tcgtcgctct aatcgctcaa gccgctctgt atttgctctg
    17401 cacgcgcgga cgctcccgcg cagagcgcgt ccggcgttgg acgcgtacac agcttttccc
    17461 gcaaaaactt ttgatattcg aggcgagcct ccgcaggtct gcccagtgcg tcgagcgtgt
    17521 gggcgatgcg cagcacgagc agttcgtccg gtgcgccgcc gcgttctgct gcgcgaaact
    17581 ctgtgagggc tgcttcgagt tggccttctt cggcaagttg gacgccgagg agtttgtgtg
    17641 tgtgcgcgtc gtcggagaga cgcagggcgg cgcggta
//
LOCUS       DASWNS010000376         4960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_143566, whole genome shotgun sequence.
ACCESSION   DASWNS010000376 DASWNS010000000
VERSION     DASWNS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143566"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VGB76_13280"
     CDS             <1..566
                     /locus_tag="VGB76_13280"
                     /inference="COORDINATES: protein motif:HMM:NF014627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEY0099914.1"
                     /translation="PYPDVTPDKLLTAVFGGFFIGAGIGLAIRGGAVLDGTEIAALLV
                     SKKSHLLRVGDVILILNIFIFSAAAFFLGIESTLYSILTYFAASKTIDFIIHGLEEYT
                     AIIIVSPHSEAIREAIILRLNRGVTVYKGRGGLFNEEQDILYCVVTRLEIGSVKNVVK
                     DIDRAAFIVTHPLADVQGGMIKKSALH"
     gene            813..1853
                     /locus_tag="VGB76_13285"
     CDS             813..1853
                     /locus_tag="VGB76_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099915.1"
                     /translation="MKKYFCVLTLACAVALAAATPTPGQTGNGRRAANSSPASTARAQ
                     QGTTAAATGLLASLPTSDAVLSVDVRRLLSDALPRAYNNNPAELTRINSEIDKFRTST
                     GLDARSVERLVVGVSYRQTTAGKTLVETVAIARGNFRNASFAPSNRPVQEQKHAGKTI
                     QIYSVDEQVKLIGLLNMRVTDVAVAMLDANTIAVGKLERVRAAIDAQAGRGRVAPEIT
                     ALATRDANALVGMGGNVPASLTQNLDMGNISKSIASVRQFYSTLGTTANGFNMLTALR
                     TENASAAQSLSTMIAGLKQLAPFALGQMPAARQRPLRSLVENTRVNTEGNDVLITLEL
                     AQVDVAALIDAF"
     gene            complement(1988..4018)
                     /locus_tag="VGB76_13290"
     CDS             complement(1988..4018)
                     /locus_tag="VGB76_13290"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0099916.1"
                     /translation="MLKQFSRLERTRKWLIVTFATLMGASLVLFYAPGGNQVNANPAT
                     NTEVIASVNRDEITVADLYRQKESERQRFGGNFSLAQVGLTDKVMLERIVSSKIMAQE
                     AARLSLGVSDAEVSKAVHTQFVDASGKFIGVDKYKDRVSQIFGSVERFERELRESLAA
                     DKLRAFVTAGVSVSEEEVREDFKRRNSTFDLTYIPVVADKLAANLQLSDEELQKYYEE
                     NKTNFRYLEPQKKIRYLYVNQVKVGERLNIPDTELRAAYDALKPENKMAGVRVQQIVL
                     KVASKELDQQVLAKATKLAQDMRGQDATVTTTTEEKFAEVAKGNSEDPATATKGGWLP
                     APVRRDPNRKNDILQATLDMQEGQVSDPVYTGGAYYIFRRGPAVPKTFEEAKRELIVS
                     QRNTRAYKAAADIAARAEQRLKETKDFQKVAQELAGEANMNAGEMVKETPFVKPGDDV
                     PDIGSSPQFEDAIKPLENANDVGSRVSVKNGFAVPMLVERREPRIPDFAEVKDEVTKR
                     ARQARAQAQLEQTARELANNNGSVADLKAAAEKLGLEVKTSEAYKLGSPLEGAGTSAA
                     ADDAIYNLKEGEVAKSPVKLGETWVVLASTKRTEADLAEFAKQRDQLTQSLLNTRRTD
                     VFEDYISSIRARLDREGRIKIYEDVLARSAEEDAASAPPTMPFPGGGIPGAQ"
     gene            4352..>4960
                     /locus_tag="VGB76_13295"
     CDS             4352..>4960
                     /locus_tag="VGB76_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEY0099917.1"
                     /translation="MEYSIWAWVGFAIFILFMLALDLGVLNRKAHVPSYREATTWSII
                     WVTLALIFAAGVFWKLGSLTGKEFLAGYLIELSLSVDNLFVFLLIFSYFKVPSKYQHR
                     VLFWGVLGALIMRILMIGIGAALINRFHWIIYIFGAFLIYTGIKMMLQKETEALPEDN
                     PIVKLVTRFIPITRHYEGENFFTTVNGKRTGTLLLLVLIIVEV"
BASE COUNT          873 a         1494 c         1487 g         1106 t
ORIGIN      
        1 tcccctaccc ggacgtgacg cccgacaaat tattgaccgc cgtgttcgga ggcttcttca
       61 tcggcgcggg catcggactc gccatacgcg gcggcgcggt gctggacggg acggagatcg
      121 ccgccttgct cgtgagcaag aagagccacc tcctgcgcgt cggggatgtg atcctcatcc
      181 tcaacatctt tatcttctcc gccgccgctt tctttctcgg catcgaatcc acgctctact
      241 ccatcctcac ttacttcgcg gcctcgaaga cgattgactt catcattcac ggactcgaag
      301 aatacaccgc catcatcatc gtctcgccgc acagcgaagc gatccgggag gcgatcattc
      361 tcagattgaa tcgcggcgta accgtctata aagggcgcgg cgggttgttc aacgaggagc
      421 aggatattct ctattgcgtc gtgacgcggc tggagatcgg aagcgtgaag aacgtggtga
      481 aagatataga cagagcggcc ttcatcgtga cgcacccgct ggcggacgtg cagggcggca
      541 tgatcaagaa atccgcgctt cattgaggtc gtttgacgcg cgccggacgt tcgcgctcgc
      601 gtgcgacgtt tgcagtcgct tttatcttgt agcgttttcc cgcgcgggaa cgtcttaagg
      661 gttgcgccgg agtctgcgag cggttcaagc caactgcggc ggcgcgtgcg gcatcggaat
      721 ggggttacac tctctcgaca atttaaattt ccacaggcga actgcaaggg gtcgtcggtg
      781 tttagcggag acggaaaggc gtgtctattt ttatgaagaa atatttttgc gttttgactc
      841 tggcgtgcgc cgtcgctctg gcagcggcga cgcctacgcc cggacagacc gggaacggtc
      901 gccgcgcggc caacagcagc ccggcttcta ccgcgagagc gcagcagggg acgacggcag
      961 cggccacagg cttgctcgcg tcgctgccga cctcggacgc ggtgttgtcg gttgacgtgc
     1021 ggcgtctgct gagcgatgcc ctgccgcgcg cttacaacaa caacccggcg gaactcacgc
     1081 gcatcaactc ggagatagat aaattcagga cgagcacggg actcgatgcg cgcagcgtgg
     1141 agcgtctggt cgtgggcgtc agttacaggc agacgacggc gggcaagacg ctcgttgaga
     1201 cggtcgccat cgcgcgcgga aactttagga acgcttcgtt cgctccgtcg aatcgcccgg
     1261 tgcaggaaca gaaacacgcg ggcaagacga ttcaaatcta cagcgtggac gagcaggtga
     1321 agttgatcgg gctgttgaat atgcgtgtga cggatgtggc cgtcgccatg ctcgacgcga
     1381 acacgattgc cgtcggcaaa ctcgaaaggg tgcgcgcggc gattgacgcg caggcggggc
     1441 gcggtcgcgt cgcgcctgag atcacggcgc ttgcgacgcg cgacgcaaac gcgctcgtcg
     1501 gcatgggcgg caacgtcccc gcgtcgctga cgcaaaatct cgacatgggc aacatctcga
     1561 agagcatcgc ctcggtgcgc cagttttaca gcacgctcgg cacgacggcc aacggcttca
     1621 acatgctgac ggccttgcgc acggagaacg cgagcgcggc gcaatccctg agcacgatga
     1681 tcgcgggctt gaagcaactc gcgcctttcg ccctcggcca gatgcccgcc gcgcgccagc
     1741 ggcctctgcg gagtttggtg gagaacacaa gggtcaacac ggagggcaac gacgtgctca
     1801 tcacgctcga actcgcgcag gttgacgtgg cggcattgat cgacgctttt taagttgccg
     1861 tcaggccgcg cgctcagacc gtgaagtgca gcgcgcaagt tagagagggg atgttcgatt
     1921 gtggtcgaac atcccctctg cttttgcgcc cgtaaggtgg agcggctgat ctctaaagat
     1981 gcgcaggtta ttgcgcgccg ggaatcccgc cgccggggaa gggcatggtc ggcggcgcgg
     2041 aggcggcgtc ctcttcggcg gagcgcgcca gcacatcttc gtagattttg attctgccct
     2101 cgcggtcgag ccgcgcgcgg atcgaactga tgtagtcttc aaagacatcc gtgcggcgcg
     2161 tgttgagcag gctctgcgtg agttggtcgc gctgcttggc aaactccgcg aggtctgctt
     2221 cggtgcgttt cgtcgaggcg aggacgaccc acgtttcgcc gagtttcacg ggcgacttcg
     2281 cgacttcgcc ttccttcaag ttgtagatcg cgtcgtcggc tgccgcgctc gtgcccgcgc
     2341 cttcgagcgg cgagccgagt ttgtaggctt cggaggtctt cacttcgagt cccaactttt
     2401 cggcggcggc tttgaggtct gcgacgctgc cgttgttgtt cgccagttcg cgcgccgtct
     2461 gttcgagttg cgcctgcgcg cgtgcctgcc gggcgcgctt cgtgacttcg tccttcactt
     2521 ccgcgaaatc ggggatgcgc ggctcgcgcc tttcgacgag catcggcacg gcgaaaccgt
     2581 tcttgacgct cacgcggctg ccgacatcgt tcgcgttctc cagaggcttg atcgcgtctt
     2641 caaactgcgg cgaactaccg atgtccggca cgtcgtcgcc cggtttgacg aacggcgtct
     2701 ctttcaccat ctcgcccgcg ttcatgttcg cttcaccggc caactcctgc gccaccttct
     2761 gaaaatcttt cgtctctttc aggcgttgtt ccgcgcgggc ggcgatgtcg gcggcggcct
     2821 tgtaggcgcg cgtgttgcgc tgcgagacga tcagttcgcg cttggcctct tcaaacgttt
     2881 tcgggacagc cgggccgcgc cggaagatgt agtaagcccc gcccgtgtaa accgggtcgg
     2941 agacttgccc ttcctgcatg tcgagcgtgg cctgaaggat gtcgtttttg cggttcgggt
     3001 cgcggcgcac gggggcgggc agccagccgc ccttcgtcgc cgtcgcgggg tcttccgagt
     3061 tccctttcgc cacttcggca aacttctctt ccgtggtcgt ggtcacggtc gcgtcctgcc
     3121 cgcgcatgtc ctgcgcgagt ttggtggcct tcgccagcac ctgctgatcc agctctttgc
     3181 tcgccacctt gaggacgatc tgctgcacgc gcacaccggc catcttgttc tccggcttca
     3241 aagcgtcgta ggcggcgcgc agttccgtgt cgggaatatt caaacgctcg ccgaccttca
     3301 cctgattgac gtagagataa cgaatcttct tttgcggttc gagataacgg aagttggtct
     3361 tgttctcttc gtaatacttt tgcagttctt cgtcggacag ttgcaggttc gcggcgagtt
     3421 tgtcggcgac gacgggaatg taggtgaggt cgaaggtact gttcctgcgt ttgaagtctt
     3481 ctctcacctc ttcttccgag acgctgacgc ccgccgtgac gaaggcgcgc aatttatccg
     3541 ccgccagcga ctcgcgcagt tcccgctcga aacgctcgac gctgccgaag atttgactga
     3601 cgcgatcctt atacttgtcc acgccgatga acttgccgct cgcgtcaacg aattgcgtgt
     3661 ggacggcctt gctcacctcc gcatcggaga cgccgagcga gagccgcgcc gcctcctgcg
     3721 ccatgatctt gctgctcacg atccgttcga gcatcacctt gtcggtcagt ccgacctggg
     3781 cgaggctgaa attgccgccg aaacgctgcc gttccgactc cttttgccgg tagaggtctg
     3841 ccacggtgat ctcgtcgcgg tttacgctcg cgatcacttc cgtgttcgtc gccgggttgg
     3901 cgttcacctg attgccccct ggcgcgtaga atagaacgag gctggcgccc atcaaggtgg
     3961 cgaatgtcac gatcaaccac ttgcgtgtcc gctccaggcg gctaaactgt ttcagcatct
     4021 tggatccttc ccgaaatcaa taacgagttt tgatgagttg atagtagcga gcgggagttt
     4081 cagagttcgc gtttctgaaa tttcaagcgg acgcgtgtgc gcagtcaaac ctcatagcgg
     4141 actctcaccc catcccatga atcgccgatt gtaacggaca cccgcacgcc ctcgcaaccg
     4201 aagagaggga tgaaggatga gggatgagag acgaatcaga aaaagcactc aagcggttaa
     4261 atgcttcttc tgattcatcc ctcatccctc atccttcatc cctttatctt gtccttgcgc
     4321 tctgcgcgac gggtgctata tgtttacggg catggaatat tcaatctggg cttgggtggg
     4381 ttttgccatc ttcatccttt tcatgctggc tctggatctg ggtgtgctta atcgcaaagc
     4441 ccacgtccct tcttatcgcg aggcgacgac gtggagcatc atctgggtga cgctcgccct
     4501 gatcttcgcg gctggcgtgt tctggaaact ggggtcgctc acgggcaaag agtttctcgc
     4561 cggttatctc atcgaactct cgctctcggt agataacctg ttcgtcttcc tgttgatctt
     4621 ctcctacttc aaagtccctt ccaagtacca gcatcgagtg ctgttctggg gcgtgctcgg
     4681 cgcgctcatc atgcgcatcc tgatgatcgg catcggcgcg gccttgatca accgcttcca
     4741 ctggatcatc tacatcttcg gcgcgttcct catctacacc ggcatcaaga tgatgctgca
     4801 aaaagagacg gaggcgttgc cggaagataa ccccatcgtc aaactcgtga cgcgcttcat
     4861 ccccatcacg cgccactacg aaggcgaaaa tttcttcacg accgtcaacg ggaaacgcac
     4921 gggcacgctc ctgctgctcg tgctcatcat cgtcgaagtg
//
LOCUS       DASWNS010000377         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_65730, whole genome shotgun sequence.
ACCESSION   DASWNS010000377 DASWNS010000000
VERSION     DASWNS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65730"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..429
                     /locus_tag="VGB76_13300"
     CDS             124..429
                     /locus_tag="VGB76_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY0099918.1"
                     /translation="MSEIFDERRKALEDEYFRRKERETLDRLRQELAAEAAASGSSAA
                     RQCPLGHGALVEEARGSVVIDRCEKCGGIWLDGGELEEVTRQSGGWFDNFVKSFRKE"
     gene            448..729
                     /locus_tag="VGB76_13305"
     CDS             448..729
                     /locus_tag="VGB76_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099919.1"
                     /translation="MNLRAATLLPECREILAATDDIEAVLLFLKQNGCGKIDSILALR
                     DLKGMNLKDGKETVHLSRTWQDRREADERLWDELFDSMKESEREHSEKD"
     gene            707..>1941
                     /gene="hutU"
                     /locus_tag="VGB76_13310"
     CDS             707..>1941
                     /gene="hutU"
                     /locus_tag="VGB76_13310"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY0099920.1"
                     /translation="MSTQRKISAPRGAKLNCKGWHQEAALRCLMNNLDAEVAERPDDL
                     VVYGGTGRAARNWAAYDAIVRELQQLENDETLLVQSGKPVAVFRTHEYAPRVLIANSN
                     LVGAWANWEHFHELERRGLMMYGQMTAGSWIYIGTQGIVQGTFETFAAMADRHFDGSL
                     SGRLVVSGGMGGMGGAQPLAATMNGAVFLGIDVDRARIERRVQTGYCDRLALTLDDAL
                     NICEDAREQQRAVSVGLTGNCAELLPEIVRRGVHVDVVTDQTSAHDPLNGYVPAGLSL
                     EEADELRRTDASGYVMRSTESMARHVEAMLALQRAGAIAFDYGNNLRKFAYDAGCADA
                     FEIPGFVPEYIRPLFCEGKGPFRWVALSGDREDIRRTDDLALELFPADSTLNRWLRLA
                     RERVQFQGLPARICWLGYG"
BASE COUNT          383 a          591 c          630 g          337 t
ORIGIN      
        1 ttttctgtta ttacaatggc gttcaaactt tgcacgcgcg ccgccgaacc tcttcctgtc
       61 atctttacga gacgcaggcg cgaccgaagc ggcgcgtgtt ccattccaga ggagagacga
      121 atcatgtcag agatttttga cgaacggcgc aaggcgcttg aagacgaata cttccggcgc
      181 aaggaacgcg agacgctcga ccgtctgcga caggaactcg ccgccgaagc cgccgcgagc
      241 ggcagcagcg cggcgcggca atgcccgctc gggcacggcg cgctcgtcga agaggcgcgc
      301 ggctcggtgg tgattgatcg ctgcgagaag tgtggcggca tctggctcga cggcggcgag
      361 ttggaagagg tgaccagaca aagcggcggg tggttcgaca acttcgtcaa gagtttcagg
      421 aaagagtagg gcgggcgttc caacatcatg aacctgcgcg cggcgactct gttaccagaa
      481 tgtcgtgaga tattggctgc cacggacgac atcgaagcag ttctgctgtt tctgaaacag
      541 aacggctgcg gcaaaatcga ttcgattcta gccctgcgcg atttgaaggg catgaacctg
      601 aaagacggca aggagacggt tcatctgagt cggacgtggc aagaccgcag agaagccgac
      661 gaacgtttgt gggacgaact gtttgattcg atgaaagagt ctgaacgtga gcactcagag
      721 aaagattagc gcgccgcgcg gcgcaaagct caactgcaag ggctggcatc aggaggcggc
      781 cttgcgctgc ctgatgaaca atctggacgc ggaagtcgcc gagcgaccgg acgacctcgt
      841 ggtctacggc ggcacggggc gcgcggcgcg caattgggcg gcttacgacg ccatcgtgcg
      901 cgagttgcag caactcgaaa acgacgagac gctgctcgtc cagtccggca aacccgtcgc
      961 cgtctttcgc acgcacgaat acgccccgcg cgtcctgatc gccaactcga acctcgtcgg
     1021 cgcgtgggcg aactgggagc actttcacga actcgaacgg cgcggcctga tgatgtacgg
     1081 gcagatgacc gccggttcgt ggatctacat cgggacgcag ggcatcgtgc agggcacgtt
     1141 tgaaaccttc gccgcgatgg ccgacaggca tttcgacggc tccctctcag gccgtctggt
     1201 agtctccggc ggcatgggcg gcatgggcgg cgcgcagccg ctcgccgcga cgatgaacgg
     1261 cgcggtcttt ctcggcatag acgttgaccg cgcgcgcatc gaacgccgcg tgcagacggg
     1321 ctactgcgac cgcctcgcgc tcacgctcga cgacgcgctc aatatctgcg aagacgcacg
     1381 cgagcagcaa cgcgccgtct cggtcggcct cacaggcaac tgcgcggaac tgctgccgga
     1441 gatcgtccgt cgcggcgtcc acgtagatgt cgtcacggat cagacgagcg cgcacgatcc
     1501 tctgaacggc tacgtgcccg ccgggttgag tctggaagag gctgacgagt tgcgccggac
     1561 ggatgccagc ggttatgtca tgcgttcaac cgaatctatg gcgcggcacg tcgaagcgat
     1621 gctcgctttg cagcgcgcgg gcgcaatcgc tttcgactac ggcaacaacc tgcgcaagtt
     1681 cgcctacgac gcagggtgcg cggacgcctt cgagattccc ggcttcgtgc ccgaatacat
     1741 ccgcccgctc ttctgcgaag gcaaggggcc gttccgttgg gtcgcgctct ccggcgaccg
     1801 cgaagatatt cgccgcacag acgatctcgc cctcgaactc tttcccgcag actcgacgct
     1861 caaccgctgg ctgcgtctcg cgcgcgaacg cgtgcagttt caaggactgc ccgcgcgcat
     1921 ctgctggctc ggctacggcg a
//
LOCUS       DASWNS010000378         4252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_430358, whole genome shotgun sequence.
ACCESSION   DASWNS010000378 DASWNS010000000
VERSION     DASWNS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430358"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2193
                     /locus_tag="VGB76_13315"
     CDS             <1..2193
                     /locus_tag="VGB76_13315"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099921.1"
                     /translation="LILASVALFIFYRLRDKPVPTVAGWRAVVNTLAGDASPGVQDAA
                     VPTQARFHNPFGVAIDAHGNVYIADAGESNRIRRIAAENSQVSTLAGGAEGFADGAGA
                     SAAFDTPSAIAIDAKGNLYVADTSNNRIRKVTPEGLVSTLAGDGTAGGRDGAAGAAQF
                     NAPVGVAVDRSGERVYVADTYNDSIRLITTGDGQVTTLAGGSATGYVDGTGKGALFDT
                     PCALVATPEGDLFVADTGNRRLRKLSRDGQVWTLQLVNADGSPFDEAFEPLGLARTHD
                     NFLYVTERERGRIWQIAPDERTTRLIAGDNPTVNDGSSDGNSARFNQPAGIAVDSRGA
                     LYVADSANYLVRKLTASGSATTAASVATVNDSTQGAAASPASVADARTPHAVESEALP
                     RLSAEAFGIKSLPWPLDPQNSWHEVSATMGEVRGSYDTSDSRHHLHSGIDIFGTYGQT
                     VRVVLDEKVESPLTNWGHGSLNEGLRVGVMVYVHLRVGRNEKDELIDEARFIPERDGE
                     GKLKGIRIKRGTRFRPGDALGTINRMYHVHMNFGAPDAETNPLRLPLQGFTDRIAPKI
                     ERDGVQLFDTESNARLTEKRDGRLLVRRGQIRIVVDAYDQVDGNQARRRLGLYKLGYQ
                     LLRADGSPAPGFPEPRVQLEFDRMPPHREAVKIAYADESGITVYGSKSTRFLYELTNI
                     VRHGRAARSAWNASELPAGDYTLRILAADFHGNEATDGRDVEIRIEDR"
     gene            complement(2335..3048)
                     /locus_tag="VGB76_13320"
     CDS             complement(2335..3048)
                     /locus_tag="VGB76_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY0099922.1"
                     /translation="MRTFYLFSLTLLAAFILCACGTRGDAPATPASDARTANAANANA
                     TPTPAGNLPKIVALGDSLTAGYGLSLHESYPSLLQEQLRRDGFAYEVVNAGISGDTTA
                     GGLRRLDWALAGDVRFVILELGANDILRGLPTAEMRKNLAQIIERSRARGAEVLLAGM
                     YAPTNSGADYSRASMEVFPALAKEYDLVLIPFFLDRVAGIENLNQADGVHPNPEGTRI
                     VASTVYQYLKPMLEKDKKR"
     gene            3047..3850
                     /locus_tag="VGB76_13325"
     CDS             3047..3850
                     /locus_tag="VGB76_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0099923.1"
                     /translation="MIELYRLSKEVPSGAETLTILHSLDLTIPDGQFVSIVGPSGSGK
                     STLLGLIAGLDAPTAGEIRIDGAAITSMDEDALAHLRGQKIGIVFQSFHLIASLTAYE
                     NVLVPMEIAGLSNAPERARRLLEEVDLTARAHHYPSQLSGGEQQRVSIARAFSNNPSI
                     LLADEPTGNLDSRNGRHVFDLMLKLNRQHNATLVLITHDHELAALADRRIALRDGRVV
                     SDSSSGLVGVDESNRTGGTERDAATGAGAEAGVDATGEDALETTEQRGR"
     gene            3847..>4252
                     /locus_tag="VGB76_13330"
     CDS             3847..>4252
                     /locus_tag="VGB76_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099924.1"
                     /translation="MRFIWQMALREIRSSWKRLLFFFLCIGIGVGAIVALRSTIQNAG
                     AAMVSEARNILTADVQVDSSREMNEATRAIIGRIAAARGVEGQTQTIEASTMLRPASD
                     AREGALMVELKGIEAPYPLYGEFKLASGERFTH"
BASE COUNT          804 a         1349 c         1377 g          722 t
ORIGIN      
        1 ctcatcttag ccagcgtcgc cctcttcatc ttttatcgcc tccgcgacaa acccgtgccc
       61 acggtcgccg gttggcgcgc cgtcgtcaac acgctcgcgg gcgacgcctc gcccggcgtc
      121 caagacgccg ccgtgcccac gcaggcgcgt ttccacaacc ctttcggcgt cgcaattgac
      181 gcacacggca acgtctacat cgcagacgcg ggcgagagca atcgcatacg caggatcgcg
      241 gcggagaact cgcaggtttc gacgctcgcg ggcggcgcgg aaggctttgc cgacggcgcg
      301 ggcgcgtccg ccgctttcga cacgccctcc gccatcgcca tcgacgcgaa aggcaacctc
      361 tacgtcgcgg acacgtcgaa caaccgcatc cgcaaagtga cgccggaagg tctggtttcg
      421 acgctcgcgg gcgacggcac ggcgggcggg cgcgacggcg cggcaggcgc ggcgcagttc
      481 aacgcgcccg tcggcgtggc cgtagaccga agcggcgagc gcgtctatgt ggcggacact
      541 tacaacgaca gcatccgctt gatcacgacg ggcgacgggc aggtcacgac gctcgcgggc
      601 gggagcgcga cgggctacgt agatggtacg gggaagggcg cgttgttcga tacgccctgc
      661 gcgctcgtcg ccacgccgga gggagacttg ttcgtcgcgg acacgggcaa ccgtcgcctg
      721 cgcaaattgt cgagggacgg gcaggtctgg acgctgcaac tggtgaacgc cgatggctcg
      781 ccgtttgatg aagcgttcga gcctttggga ctcgcgcgca cgcacgacaa ctttctctac
      841 gtgacggagc gcgagcgcgg gcgcatctgg cagatcgcgc ccgacgaacg cacgacgcgc
      901 ctcatcgcgg gcgacaaccc caccgtcaac gacggaagca gcgacggcaa cagtgcgcgc
      961 ttcaatcaac ccgcaggcat cgcggttgac tcgcgcggcg cgttgtacgt cgccgatagc
     1021 gcaaattatc tcgtgcgtaa gttgacggcg agcgggagcg cgacgaccgc cgcgagcgtc
     1081 gccacggtta acgattcgac acaaggcgcg gcggcctcgc ccgccagtgt tgccgacgct
     1141 cgcactccac acgcggttga gagcgaagcg ttgccgcgcc tgtcagccga ggcgttcggc
     1201 atcaagtctt tgccgtggcc gctcgacccg caaaattcat ggcatgaagt ctccgcgacg
     1261 atgggcgagg tgcgcggcag ttacgacacg agcgatagcc gccaccacct gcacagcggc
     1321 atagacatct tcggcacata cgggcagacg gtgcgcgtcg ttctcgacga aaaggtggag
     1381 agtcctctga ccaactgggg gcacggcagt ttaaacgagg gcttgcgcgt cggcgtgatg
     1441 gtctacgttc acctgcgcgt cgggcgcaac gagaaagacg agttgattga cgaagcgcgc
     1501 tttatcccgg agcgcgacgg agaagggaaa ctcaagggca tccgcatcaa acgcggcacg
     1561 cgctttcgcc ccggcgacgc cctcggcacg atcaatcgca tgtatcacgt ccacatgaat
     1621 ttcggcgcgc cggacgccga gacgaacccg ctccggcttc cgctccaagg cttcacagac
     1681 cggattgcgc cgaagatcga acgcgacggc gtgcaactct tcgacacgga gagcaacgcg
     1741 cggctcacgg aaaagcgcga cggtcgcctc ctcgtacgtc gcgggcaaat tcggatcgtg
     1801 gttgacgcct acgatcaagt tgacggcaac caggcgcgcc gtcgtctcgg cctctacaaa
     1861 ctcggttacc agttgttgcg tgcggacggc agccccgccc ccggcttccc ggagccgcgc
     1921 gtgcagcttg agttcgaccg gatgccgccg catcgagaag ccgtgaagat cgcctacgca
     1981 gacgaaagcg gcatcaccgt ctacggcagc aagagcacgc gcttcctcta cgagttgacg
     2041 aacatcgtcc gccacggtcg cgccgcgcgc tccgcgtgga acgcctccga actccccgca
     2101 ggcgactaca cccttcgcat cctcgccgca gacttccacg gcaacgaagc gacggacggg
     2161 cgcgatgtcg agatcaggat tgaagacaga tgatgaagga ggagcgaaag caggagtcag
     2221 aaaacagaat acagaagtca gaatagaaca ggcacgaatc ttcctcctga ctcctgactt
     2281 ctgactcctg acttctgttt tctcgtccct ccttccgcct tcatccttcc gccttcatcg
     2341 tttcttatct ttctccagca tcggcttgag gtattgatag acggtcgagg cgacgatgcg
     2401 tgtgccttcg gggttgggat ggacgccgtc ggcctgattg aggttttcga ttccggcgac
     2461 gcggtcgagg aagaagggga tgagcacaag gtcgtattct ttggcgagcg cggggaaaac
     2521 ttccatgctg gcgcggctgt agtctgcgcc tgagttcgtc ggcgcgtaca tccctgcgag
     2581 caggacttcc gcgccgcgtg cgcgcgagcg ttcgatgatc tgcgcgaggt tcttgcgcat
     2641 ctctgcggtg ggcaggccgc gcaggatgtc gtttgcgccg agttcgagga tgacgaaacg
     2701 cacgtcgcct gcgagcgccc agtcgagacg gcgcaagccc ccggcggtcg tgtcgcccga
     2761 aattcccgcg ttgaccactt cgtatgcaaa accgtcgcgc ctgagttgct cttgtaagag
     2821 cgacgggtaa ctttcgtgaa gagataagcc gtatcctgcc gtcaggctgt cgccgagcgc
     2881 gacgatcttc gggaggtttc cggcgggcgt cggcgtcgcg ttggcgttgg cggcgttcgc
     2941 ggttcgcgcg tcgctcgcgg gagtcgcggg cgcgtcgccg cgtgtgccgc aggcgcagag
     3001 gataaaggcg gcgagtagtg ttaagctgaa taaataaaat gtacgcatga ttgaattata
     3061 cagactcagc aaagaagttc cttccggcgc ggagactctg acgatcctgc actcgctcga
     3121 tctgacgatc ccggacgggc agttcgtctc catcgtcggc ccttcgggga gcggcaagtc
     3181 aaccctgctc ggactcatcg ccggactcga cgcgccgacc gccggagaga tacgcattga
     3241 cggcgcggcc atcacctcga tggacgaaga tgcgctcgcg cacctgcgcg gccagaagat
     3301 cggcatcgtc tttcaatcct tccatctcat cgcttcgctc acggcctacg agaacgtgct
     3361 cgtgccgatg gagatcgcgg gtttgagcaa tgcgccggag cgtgcgcgca ggttgctcga
     3421 agaggttgac ctgacggcgc gcgcgcatca ttacccgtcg cagttgtcgg gcggcgagca
     3481 gcagcgcgtc tcgattgcgc gcgccttctc aaacaaccct tcgatcctgc tggccgacga
     3541 gccgaccggg aatctcgatt cacgcaacgg tcgccacgtc ttcgatctca tgttgaaact
     3601 caatcgccag cacaacgcga cgctcgtgct catcacgcac gaccatgaac tcgccgcgct
     3661 cgccgaccgc cgcatcgcgc tgcgcgatgg ccgcgtagtc agcgacagca gcagcggcct
     3721 cgtcggcgtg gatgagtcga ataggacggg cggaacggaa cgcgacgcag cgacgggcgc
     3781 aggcgcggag gcgggcgtgg acgcgaccgg cgaagacgct ctggagacga cggagcagcg
     3841 agggcgatga gattcatctg gcaaatggcg ctgcgcgaaa tacgctcttc ttggaagcgc
     3901 ctgctctttt tcttcctctg catcggcatc ggcgtcggcg caatcgtcgc gctgcgttcg
     3961 acgattcaga acgcgggcgc ggcgatggtt tccgaggcgc gaaacatcct caccgcagac
     4021 gtgcaggtcg attcgtcgcg cgagatgaac gaggcgacgc gcgccatcat cgggcggatc
     4081 gcagccgcgc gcggcgtcga gggacagacg cagaccatag aggcttcgac gatgctgcgt
     4141 cccgcttcgg acgcgcgcga aggcgcgttg atggtggagt tgaaaggcat cgaagcgccc
     4201 tacccgctct acggcgaatt caaattggca agcggcgaga ggttcacaca cg
//
LOCUS       DASWNS010000379        22917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_274107, whole genome shotgun sequence.
ACCESSION   DASWNS010000379 DASWNS010000000
VERSION     DASWNS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22917
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274107"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..2475)
                     /locus_tag="VGB76_13335"
     CDS             complement(88..2475)
                     /locus_tag="VGB76_13335"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0099925.1"
                     /translation="MRKVYRRYQVVFVSIAFVVAAAGALLLPFASASCSAPPPNQAGE
                     PEAIERLRALTHGVPPAALPAESAVASIETQYARTAGGALARMVRARIKLAAGDTAGA
                     VALLDSDAFRQQTALGDYALLLRADALAKAGRRTEARAAYEQLARDYPRSIRAREAAL
                     RNAEIALQENAASAVPLLVKSLAEADDGGALLLTAKAQEQTGDTTRALAAYRRLYFFA
                     PASPGAAEAASALARLGSTLAPASANEATTRAHKLYEAKRYGEAAAAYGDAFTRFPPT
                     ADTGSQLRRGIAAFNAGTSRLPEAVAALNAIPTSAGEARVEALFYLVQAHAKSRQWEA
                     ARTVHAEMRRAFPEHTYTKRALAAAGQIARDAKNTFEAANFYRAAVAAYPGAAEVAQA
                     QFELAWAAHESKNYTESARLLLEHLAAYADRNTDNRGRAGYWAARDAERAGQFERART
                     LYEAMLQRYDANWYGDLARQRLDALKRTHPVAATGYVPEPQVARAVVNLSTVSVAEET
                     ATADANEALAKADQLNVVGLDEYALDEVNKVLESAPRSPRLNLAKARIYRTRQQNLHA
                     FLALQKSYPDYSQMKPEELTREEWDVFYPLAYWETITREARAKSIDPYRVAGLIRQES
                     VFDPRAASHANAYGLMQLLLDTAQRTARKYGITRAITKDVLLSDPQLNIQIGTGYLRD
                     QLNTFGRIEYVAAAYNAGPGRAVRWRAELPAEMDEWAEAVPFRETRGYVQGVVRNTLQ
                     YKRLYDEQGQFRVEVGARALRPAATGTDAPAAQPSNATVRPRRATPDEDDLQEEQ"
     gene            complement(2772..4214)
                     /locus_tag="VGB76_13340"
     CDS             complement(2772..4214)
                     /locus_tag="VGB76_13340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0099926.1"
                     /translation="MAGSEDLEQSLRAEVENYIRARVQGQQEEIARLQSQVNEAFTRL
                     SERLSDASLSDAPIAISISEHLRAATGKGTEAAAQTSARARATSDIALLKAAVDDIDN
                     QRSQADILNTLVNRAASFTPRLAFFVVKNERATGWRARGLEGTVGDDAVREISLPLSA
                     ETLLSRAVAARSTWSGEPGTNADDHQLISKLGDEPPQRMVAIPLIARGKCVAVLYADS
                     AGLEPDAINLEALETLVRVTSMSVELLAVTRAAPRPAAERTHAPSASVSVEASAPAAG
                     REAVSAPQQAEPAQAPNAGTAQPHEPAFAAAPQVERGIEPAYATEAMRPPVAPETFGE
                     RVTPTPSATLAVEPEATSAPPPPVAPPPVAAPLGGSSRRSGADAELPVEVSEEERRYH
                     NDARRFARLLVSEIKLYNEQKVRDGREQGNLYERLREEIDRSRQMYDKRVAANVASRY
                     DYFHHELVNTLAEGNSEKLGGGYPGASAGV"
     gene            4641..5048
                     /locus_tag="VGB76_13345"
     CDS             4641..5048
                     /locus_tag="VGB76_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0099927.1"
                     /translation="MPPTSILIADDYDDNRELLRLMLEGAGYSVRETRDGRECVAAAR
                     AEPPDIVLIDLSMPVLDGWATLRELRADERTRTLACVAVTALAAEQDRTRALDAGFDA
                     YLAKPYRSRDLLELVERLLRQTRTATDASRQNL"
     gene            5045..5356
                     /locus_tag="VGB76_13350"
     CDS             5045..5356
                     /locus_tag="VGB76_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099928.1"
                     /translation="MTDDSRHSGSSSRTEKDDEDELTPGRILVAFEKLFGGGRGVQKF
                     ARSGLRYMRLAGGAVLVEQNPKKKSEWAKLAQAGRRVAWVMHEGEYLARVVDGEVSML
                     D"
     gene            complement(5387..6565)
                     /locus_tag="VGB76_13355"
     CDS             complement(5387..6565)
                     /locus_tag="VGB76_13355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0099929.1"
                     /translation="MQQTADVVIIGGGIVGASVAFHLAERGCTDVVVVEREAAQGLGS
                     TGKATGGVRAQFATAINIRMSRYSIDFFTRFEETTGHACGYEAAGYLFVATSAEQFDY
                     LQRTRERQLAEGLKNVELVGADEIARRVPGLRTDDVTGGSFCQTDGFIAPLRVLGGFT
                     ARATARGVRVQTETEVTGIEVEGGRVAGVQTTRGRISTRAVVNASGAWAAETARMAGV
                     EIPVVPLRRQLASVRPAAPVPEGLPMVIDMSNGFHFRPEARELESPGVLLAWPDETET
                     PGFKTDFDPLFTAKVLQRARERVPHLVHGATLNESRCRAGLYELTPDHHAIIGEAPGV
                     CGLFLANGFSGHGVMHAPATGRILAELILDGRAELMDVSALGAERFAQGRLIEETSVL
                     "
     gene            complement(6570..9074)
                     /locus_tag="VGB76_13360"
     CDS             complement(6570..9074)
                     /locus_tag="VGB76_13360"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEY0099930.1"
                     /translation="MNATLHHNRQLRAVACALLFAFSFAAFEARGAWRAAGDVVAVER
                     ETNGVVLTLSSGARARVTFFDLDVVRVRFAPRGVFERDFSYATWARDRKTVAASIKET
                     RETIEITTRVDGGKIVIRRRPFALTILDAGGNVLLEDERGRPVSFDLESGAIEASKRR
                     DALEFYYGFGEKALPTSRQGQSIVNWNTDAFGYTPGLDPIYQSIPFFIALRQGRAYGL
                     FFDNTYRTHFDMGHSAPERYTFGAAGGELNYYVFTGGRERTPQKVLADYTELTGRMPL
                     PPLWSLGYQQSRWSYFPEAKVREIARGFRERKIPADVIYLDIDYMDGYRVFTWDKERF
                     PDPPKMLSDLRRDGFRTVLIIDPGIKVDENYFAYQAGRAGGFFHRKPDGAEFRANVWP
                     GVCAFPDFTSAQARSWFGSLYAKHLDEGVSGFWNDMNEPATFPPDTRPPQPNVMHDPA
                     KTFPLDVRHAGDGQPGDHARYHNVYGMQMARSTFEGVQRLRPEARPFVLTRAGYAGVQ
                     RFSAVWTGDNVPSWEHLQLSIAMLTNLSISGVPFVGADVGGFTGNASAELYTRWLQAA
                     ALTPFYRSHISARLQEREPWSFGETHERINRASIELRYQLLPYIYTLFREHERTGMPV
                     MRPLWFNYPADYNTYAPALPAEQFLLGDDLLVAPVVVQGETKRPVYFPKGDAWIDWWT
                     GARYEGGTTAEIDAPLARLPLFARAGAIIPMQAVVQHTGEMARAPLSVKIISGADGVG
                     RIHEDEGDGYGYRRSAWRATTVTQSAGAIRFKRVGDFGGARPITSVEVLGLETRPKEI
                     RIDGRDAARNVSFERDERRLRFALPQGNVSEIVFVP"
     gene            complement(9187..11001)
                     /locus_tag="VGB76_13365"
     CDS             complement(9187..11001)
                     /locus_tag="VGB76_13365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0099931.1"
                     /translation="MKLLRTTLALSLVALLLCSTHDSTRAAFNASSTLPPQRAVSLVL
                     TNGKIFTADARGTLAEAVAIEADRIVAVGTNAEINRAYRGARTIDLRGRLVTPGFNDA
                     HLHFIGGGLSLLRVELVGARTLEEAKARIAAKVRELPAGAWILGRGWDHTLWGGQWPT
                     KADLDAVAPNNPVYVQRVDGHVSWANSLALKQAGVTRETPVPEGGEILRDQDGQPTGI
                     LKETAAGLVGRVVPQPSRAERMQAAERALAEARRYGITSIQDNSGYESIELYRELLQA
                     GKLTVRVAEWQNFEDTVERLKAQRAEFASFKIDPLRIRLTALKGYVDGTLGSRTAAML
                     APFADDPHNSGIPRRPPEELTRMIVERDAAGFQIALHAIGDRANRMALDGFKAARSIR
                     DEKPAQAPSPEPAVRQANPAKPGDTQAARADIENEAGLNKFRHRIEHSQVVAPSDFAR
                     FRSLGVVASMQPSHAISDKRWAQDRLGEYRVQGAYAWHKMQAHGVHVAFGTDWPVEPI
                     NPYLGLYAAVTRRSTEGDPAGGWWPQERLSIAEAIRCYTAGSAYASFEEEQKGQLAPG
                     MLADLVVHSRDLLTIKPGEILQTEAAITILGGRVVYEK"
     gene            11410..12390
                     /gene="galE"
                     /locus_tag="VGB76_13370"
     CDS             11410..12390
                     /gene="galE"
                     /locus_tag="VGB76_13370"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY0099932.1"
                     /translation="MRVLVTGGAGYIGSVVTEELVRDGHEVVVYDSLYKGHRGAVVEG
                     ATLIEADLMDGERLRRAFREQRTEAVIHLAADSLVGESVMNPAKYYRNNVVAGLALLD
                     AMNECEVRRLVFSSTAATYGEPEKQPIEESDPTNPTNTYGQTKLAFEHALPWYECAHG
                     MHYASLRYFNAAGATEHCGEMHDPETHLIPIILQAAKGTREAVEIYGEDYPTRDGTCV
                     RDYIHVVDLARAHILALGILDERSAIYNLGCGGDGYTVREVIDTARKVTGREIKVRVG
                     ARRAGDPAFLVASSARIKRELGWSPQFQDLHLIIESAWAWMERHPRGYEE"
     gene            12380..12550
                     /locus_tag="VGB76_13375"
     CDS             12380..12550
                     /locus_tag="VGB76_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099933.1"
                     /translation="MRNRGDASRAPALEPGGEERAGKKAGGAQSKYCAPPALFMGWNE
                     GAQSVRARLRRV"
     gene            complement(12547..16626)
                     /locus_tag="VGB76_13380"
     CDS             complement(12547..16626)
                     /locus_tag="VGB76_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEY0099934.1"
                     /translation="MNYRKILPSLVVLTLLAVLILPLVVVTADSSAIRFEPTAYAPGS
                     IHNQDGWSSLGSAGLGCAQYDHAVVSNSGAPAAFGTQSLRMSNAVTSGCFQDGTFSKS
                     VVNEAGETGAENAGLSRGVRQNYFEAEWQFASTVPGSEQPGLSVVASPDRGDGARMSW
                     VQMLDTPSGLEVNFFDYQRGQNDFVQTNLTSGLSRAVPHTIKLTMNFVDGDSNDVVRV
                     YVNGVLEHTGTSWEDYYRDVEENPTRTVDSILFRTSGAPAPATAGQGFLIDNMLLNSA
                     NDADADGDPDSTAVLVLPANLNGWFFFNEGANGSGGFEQGPATPPLGGGSANLTVDAT
                     GRHNLGTQAYAGTRLDQISQLRYSTYKNTNPDQAVDIALQFDIDYDGAGGNAAYQGRL
                     VFEPYQSGTNPQQNVWQTWHALAGRWYASRPGANGSNGVCPQNAPCTWPQVLASFPNA
                     TIFHGGTPQTAGVLLFRAGGPWANGYDGNVDAFTIGIADAYTTFNFEPDLDLDGVPDG
                     ADNCPNTPNPGQEDEDQNGIGDACQSPSGGRIRQVDDDRVQCPFAAHTSITAAVAAAS
                     NGDQIDVCAGTYTETVTVNKALNLRGAQAGVDARTRAVPVTDESVLNSPGGSFNITAS
                     NATVDGFTVQEGSAAPLANGITLAANRSGHRIFNNIIRDNTFGLYLNSNGAIQSIVRF
                     NRFEGNNRAGAASGNAIYSDQGASNVVINQNRFTGHTSAAMVFAGTQSNITVSSNQLV
                     NDNSMVFFNSSAIIITGNVVTGSQGSVIFIGGGNNGIVITCNAISGGAANTSAIRIPN
                     FGSGTNSNVTANFNNIETMPFGIRIDTGQYTGTLNAENNYWGAPNGPTDEQGRNPAGT
                     GVVIRDPNFVVDFIPFLTAPLPDSDGDGAPDGCDTDDDDDGIPDTSDNCPTVANQDQT
                     DTDGDGQGNACDPDDDNDGVPDGSDNCRYLANPDQADTDDDGRGNACPLPVAGQILIS
                     EFRFRGQGPPGVSDGLFDEFVEIYNNTNVPFTVDTADNSAGWAVVAPNGGVRFVIPAG
                     TLIPARSHYLGAHALYALSTVAAPNMTYETEIEDDTGIALFGSSTTFTPTTRFDAVGF
                     TTASPLYYEGTALPPVGSVDGDYSFARKLVTGLPQDTNDNAADFVFVSTSAAQFNGVQ
                     SVLGAPGPENLASPIQRNSVIKASSIDTCAGAGTCENRRRIGTPVPNGDFGTLKLRRK
                     FTNTTGGIVTRLRFRVVDITTLNSPGYAPGNGQADLRVVPSDASNFNVTLSTGAVVPV
                     SGTQAGQPPNQPLGGGVNSTVEFIAPVQIASGASVNVEFNLGVMQNGFFRFLVNVEAV
                     ITPSASTSEAAAAMKTPATKKSAKPGAAAATRQGAKRKR"
     gene            complement(16926..17879)
                     /locus_tag="VGB76_13385"
     CDS             complement(16926..17879)
                     /locus_tag="VGB76_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY0099935.1"
                     /translation="MRFPFNLPAGREFEAVGFGLNAVDHLIVVDAYPAFDTKTRFKTH
                     VQAAGGQTASAMVALKRLGLPTAYAGRFGSDAEGEFGLATLVAEGVNVEHAQQVEGAR
                     NQIAFIIIDAGTGERTILWERDARLAYAAEDAPAGFAARGRVLHLDAHDPPACARLAR
                     EARAAGTIVSADIDNIYEGLPELLPLLDVLITSSEFPRRLTGIPNERAALKEVKARLH
                     EKAVVGMTLGVRGALVYHEGAYHEADSFPVPGGCRDTTGAGDAFHAGFLYAMLQGETI
                     EACLNTGCAVAALKCRDLGARAALPTAHELNQFLTAEGETS"
     gene            18072..20201
                     /gene="uvrC"
                     /locus_tag="VGB76_13390"
     CDS             18072..20201
                     /gene="uvrC"
                     /locus_tag="VGB76_13390"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY0099936.1"
                     /translation="MTLEEKLKNLPLSPGVYIHKDEAGKIIYIGKAKNLRNRVRSYFQ
                     SGRGHDRKTRELVRRIRDLEFIVTDTEVEALVLESNLIKKHKPRYNVQLKDDKQYPHL
                     KLTINEPFPRVMITRRVQRDGALYFGPFLPASLARRTIDLINRTFQLRTCDIEIDGKL
                     PRPCLEYHIKRCLGPCVRGLCTPEEYAESVRDVRLFLEGKDKQLADEYAGRMERASEE
                     MRYELAAKYRDLRKTVLAVSETQKMATSPERDVDIFGYYREAQRLALQLFTMREGKIV
                     GRREFFWEDLSADEFDPAEFLSEVLTQYYSTDYVPREIHVPVDFEDRELLERALSERR
                     GRRVRILDPQRGEKRDMIALVEKNAQIAFEQRFRVLKPDMERVLEELQETLELPRFPA
                     RIESFDISNISGAENVAAMVVCENGKMNRAEGRRFKIKTVEGSNDVASMREAVARRYR
                     RQLDENNPLPDLVMIDGGKGQLNAAADAMRELDLEAIPMIGVVKPPRRHNEVSHLLVK
                     GREHEPVYLDSHSAVLRLIQMIRDETHRAAVTYHRKRREIRDFTSELTEIPGVGDKRK
                     NRLLRNFGSIARIAQATAAELAPFIGRQTAEEVAAHFTRQRALAGGDNNNGGDDAGAE
                     SVAPSLATTGSVGARDTDDADEMPLEVETRLDDPEGDAEDLQPIRSVDHAGEVRPPRR
                     KSTRKRGERSTNPHGIKRERIGKSETE"
     gene            20239..20796
                     /locus_tag="VGB76_13395"
     CDS             20239..20796
                     /locus_tag="VGB76_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0099937.1"
                     /translation="MNPPTHIETARLILRKPVLTDTPLIYEAYARDAEVTRYLTWRPH
                     RTLADGDEPAAQRLAAWEAGRYFSWVIILRSGGELAGMISARPDGWRVDLGYVLGRRW
                     WGQGYMTEAVRAVSDWALSEREVFRVWAVCDVDNPASARVLEKAGMRLEGRLRRWALH
                     PNMSDEPRDCLCYARVKEDDGSRSS"
     gene            20853..22415
                     /locus_tag="VGB76_13400"
     CDS             20853..22415
                     /locus_tag="VGB76_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0099938.1"
                     /translation="MKTDNGQTTSVWMQTADVPEGLPLGADAQADVVIVGAGIAGLTT
                     AYLLTKEGKRVVVLDDGPIAGGETCRTTAHLSNALDDRYYELERLHGEDGARVAAESH
                     TAAIDQIEAIVNEEKISCDFARVDGYLFVPPGDDPAQLDDELKAAHRAGLTEVERVER
                     APLDSFDTGTALRFPRQGQFHILKYLNGVAAAVRRSGSEIYTKTHAIKIEGGEEGARV
                     ETTNGASVTAKAVVVATNSPVNDRVTMHTKQAPYRTFVVGARVPRGSVPQILLWDTPD
                     PYHYVRLQRGSDGKGENEGGAYDVLIVGGEDHKTGQADDAEARFACLEEWTRERFPQV
                     ESFEFRWSGQVMETVDGLAFIGRNPLDSDNVYIATGDSGHGMTHGTIAGMLLTDLILG
                     RENRWAELYDPSRKPLSLSAAKDFAGENLNVAAQYTDHVTPGEVSDESEIKSGEGAII
                     RRGLTKVACYRDESGALHERSAICTHLGCVVQWNSTEKTFDCPCHGSRFQKDGHVVNG
                     PAISALAEVEKS"
     gene            complement(22600..>22917)
                     /locus_tag="VGB76_13405"
     CDS             complement(22600..>22917)
                     /locus_tag="VGB76_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099939.1"
                     /translation="VDEQKFHAFISRTSLYYHAIFGWGRTLSGLSPNRAPVTPAEIET
                     ERSAYAGYISNFTRERAAQLPLAYVVATAGQSDLTNLDRWYTRDAGERIGNYILYRVR
                     LRP"
BASE COUNT         4056 a         7008 c         7675 g         4178 t
ORIGIN      
        1 tgtctctttc gtcgtttcaa acatgtgcgc ttcctctagt tttaccggcg atgacggttc
       61 actgcttcta tgctctttcg tcgtttctca ttgctcctct tgtaagtcgt cttcgtcggg
      121 agtcgcacgg cgcggacgga cggtggcgtt tgaaggctgc gcggcgggcg cgtccgttcc
      181 cgtcgcagcc gggcggaggg cgcgtgcgcc gacttccacg cggaattgtc cctgctcatc
      241 gtagagccgt ttgtattgca gggtgtttct gacgacgccc tgcacgtagc cgcgcgtttc
      301 gcggaagggc acggcctccg cccactcgtc catctcggcg gggagttcgg cgcgccagcg
      361 cacggcgcga ccgggtcccg cgttgtaggc ggcggccacg tattcgatgc gcccgaaggt
      421 gttgagttga tcgcgcaggt agcccgtgcc gatctggatg ttgagttgcg ggtcggagag
      481 caaaacgtcc ttcgtgatgg cgcgcgtgat gccatacttg cgtgcggtgc gctgcgcggt
      541 gtcgagcagg agttgcatga ggccgtaagc gttcgcatga gacgcggcgc gcgggtcgaa
      601 gacggactcc tgccggatca gcccggcgac gcgatacgga tcgatgctct tggcgcgcgc
      661 ctcacgcgtg atcgtctccc agtaggcgag cgggtagaag acatcccact cttcgcgcgt
      721 gagttcttcc ggcttcatct gcgaataatc gggataactt ttttgcagcg cgagaaaggc
      781 gtgcaggttc tgctggcgcg tgcgatagat gcgcgccttc gccagattca gacgcgggct
      841 gcgcggcgca gactccaaaa ccttattcac ttcatccaac gcgtactcgt cgaggccgac
      901 gacgttcagt tggtctgctt tcgccaacgc ttcgttcgca tctgccgtgg cagtctcttc
      961 ggctacggag actgtgctga gattgacgac ggcgcgcgcg acctgcggct ccggcacgta
     1021 tccagttgcc gcaacggggt gcgtgcgttt cagtgcatcg agtcgctggc gtgcgagatc
     1081 gccgtaccag ttggcgtcgt agcgttgcag catcgcttcg tagagcgtgc gcgcacgttc
     1141 gaactggcct gcgcgttcgg cgtcgcgcgc cgcccagtaa ccggcgcgcc cgcgattgtc
     1201 cgtgttgcgg tcggcatagg cggcgaggtg ttcgagcaga aggcgcgcgc tctccgtgta
     1261 gttcttcgac tcgtgcgccg cccaggcgag ttcaaactgc gcctgcgcca cctcggctgc
     1321 gcccggatag gcggcgaccg ccgcgcggta aaagtttgcg gcctcgaagg tgttcttcgc
     1381 gtcgcgcgcg atctgcccgg cggcggccag cgcgcgtttc gtataagtgt gttcgggaaa
     1441 agcgcggcgc atctccgcgt gcacggtgcg cgccgcctcc cattgacgcg acttcgcgtg
     1501 agcctgcacg agatagaaga gggcttcgac acgcgcctca cccgccgaag tgggaatcgc
     1561 gttcaaggcc gcgacggctt cgggcagacg gctcgttccg gcgttgaaag cggcgatgcc
     1621 gcgacgcaac tgcgacccgg tatcggcggt aggggggaaa cgtgtgaaag cgtcgccata
     1681 ggcggcggcg gcttcgccgt aacgcttcgc ttcataaagt ttgtgagcgc gcgtcgtcgc
     1741 ttcattggcg gacgcgggcg cgagcgtcga accgaggcgc gcgagcgcgc tcgcggcttc
     1801 ggcagcgccg ggcgaggcgg gcgcgaagaa gtagaggcga cggtaggcgg cgagcgcgcg
     1861 cgtcgtgtcg ccggtctgct cttgagcttt cgcggtcagg aggagcgcgc ccccgtcgtc
     1921 ggcttccgct aaacttttga cgagcagcgg cacggcggac gccgcgttct cttggagggc
     1981 gatttcggcg ttgcgtaaag cggcttcgcg tgcacgaatc gagcgcgggt agtcgcgcgc
     2041 gagctgttcg taagcggcgc gcgcttcggt gcggcgtccg gctttggcga gcgcgtcggc
     2101 gcgcaggagc agcgcgtagt cgccgagcgc ggtctgttgt cgaaaggcgt ccgagtcgag
     2161 cagcgcgact gcgcccgccg tgtcgcccgc cgcgagcttg atgcgcgcgc gcaccatgcg
     2221 cgccagcgcg ccccctgccg ttctggcata ctgcgtctcg atgcttgcca ccgcgctctc
     2281 cgcaggcagc gcagccggtg ggacgccgtg cgtcagagcg cgcagacgct cgatggcttc
     2341 gggttcgccc gcctgattcg gaggcggcgc gctgcaactc gcgctcgcaa acggcagcag
     2401 caatgctccc gcagcggcga cgacaaacgc gatggagacg aagacgactt gataccggcg
     2461 ataaactttt ctcaaaactt ttccactccc acgccctgcg agatgaacaa ctcgctccgg
     2521 cagttgaaac ttacagcccg ttccctatag ctcgctgcta cgacaccgct taagctaact
     2581 tgctgcgacc ggggagagca agttgaggct ggccggaacc cgcgcgcccg gtgcctccgg
     2641 acttcgctag gatgcggcga cttgtgaagg ccgttcgccg cgaaagaaat gtgaaggccg
     2701 ttcgctccga aagagaaagg gcggggagtt taagccccgc cctgcaaaga gtctcgcgtt
     2761 gctgcgcgtc gtcagacgcc cgcgcttgcg cccggatagc cgccgccgag tttttcggag
     2821 ttgccttcgg cgagcgtgtt gacgagttcg tgatggaaat agtcgtagcg cgaggcgacg
     2881 tttgccgcga cacgcttgtc gtacatctgg cgcgagcggt cgatctcttc gcgcaggcgt
     2941 tcgtagaggt tgccctgttc gcgcccgtcg cgcaccttct gttcgttgta gagtttgatc
     3001 tcggagacga gcaggcgcgc gaagcgacgc gcatcgttgt gataacgccg ctcctcttcg
     3061 ctgacttcga cgggcaactc cgcgtccgcg ccggagcgac gcgaactgcc cccgagcggc
     3121 gcggcgacgg gcggcggggc gacaggtggc ggcggcgcgc ttgtcgcctc cggttcgacg
     3181 gcgagcgttg ccgacggggt cggcgtcaca cgttcgccga aagtctccgg cgcgacgggc
     3241 ggcctcatcg cttcggtggc gtaggccggt tcgatgccgc gctccacttg cggtgcggcg
     3301 gcgaacgccg gttcgtgcgg ctgcgccgtt ccggcgttcg gagcctgcgc gggttcagcc
     3361 tgttgtggag cggaaactgc ttcccggcct gcggcgggcg cgctcgcttc gacggagact
     3421 gacgccgacg gcgcgtgcgt gcgttcggct gcgggacggg gcgcggcgcg tgtcacggcc
     3481 agcagttcga cggacatgct ggtgacgcgc acgagcgtct ccaaagcttc gaggttgatc
     3541 gcgtcgggtt cgagtccggc gctatcggca tacagcacgg cgacgcattt gccgcgcgcg
     3601 atgagcggga tggcaaccat gcgctgcggc ggttcgtctc cgagcttcga gatcagttgg
     3661 tggtcgtctg cgttcgtgcc gggttcgccg ctccaggtcg agcgcgctgc gacggcgcgg
     3721 ctcaagagcg tctcggctga gagcggcagc gaaatttcgc gcaccgcatc gtcgcccaca
     3781 gtgccttcga ggccgcgtgc gcgccagccg gtcgcgcgtt cgttcttgac gacgaagaag
     3841 gcgagacgag gcgtgaacga ggcggcgcgg ttgacgagcg tgttgagaat gtcggcttgc
     3901 gaacgctggt tgtcgatgtc gtcaacggcg gctttcagga gcgcgatgtc gctcgtcgcg
     3961 cgggcgcggg cggaagtttg cgccgccgct tccgtcccct tgccggtcgc ggcgcgcagg
     4021 tgctccgaga tcgagatggc aatcggcgcg tctgagagcg aagcgtcgga gaggcgttcg
     4081 ctgaggcgcg tgaacgcttc gttgacctga ctttgcagcc gcgcgatctc ttcctgctgc
     4141 ccctgcacgc gcgcgcggat atagttctcc acctcggcgc gcaaactctg ttccaagtct
     4201 tcgcttcctg ccacctgtga ctctccttct gtcgtcgtgt tgttcgatag tgcgggctgg
     4261 ctcgcgcccg gtaggtcgcg gggtctgtca ggtgtttttg actgaccgga ggcgcgcgct
     4321 gacaagagcg caagtgccag tcgaggatta tggcgggcgg gcgcgcgcac tgtcaagcaa
     4381 ctcttgaaac ggggaaagga ggaagggaga aaggtgaagg tggcggcaaa agtgtagatg
     4441 aatagtgggc gcggttcgcg ctatccgctc gctgcgttcc gctctccctt ctcccgcccc
     4501 aatgtttgac acgcggcgcg ctctacgtta taacgggcgc gcctctccgt cgttactgct
     4561 gttgatccac tgtgcggaag catatccggc gaaccctgaa agggttgacg cacgacccgc
     4621 tctcatgagc cgaaacgaca atgcccccaa caagcatcct gatcgctgat gattatgacg
     4681 ataatcgtga actcctgcgc ctgatgttgg agggcgcggg ctacagcgtg cgcgagacga
     4741 gggacgggcg tgagtgtgtg gcggcggcgc gcgccgaacc gcccgacatc gtgctcatcg
     4801 atctctccat gcccgtcctc gacggctggg cgacgctgcg cgaactgcgc gcagacgaac
     4861 gcacacgcac gctcgcctgc gtcgccgtca cggctctggc cgccgagcaa gaccgcacgc
     4921 gcgcgctcga cgcgggcttc gatgcctatc tcgccaaacc ttatcgctca cgcgatctgc
     4981 tcgaactcgt cgaacgcctc ctccgccaga cccgcaccgc gaccgacgct tcgcgccaaa
     5041 atttatgacc gatgattcgc gccactccgg cagcagcagc cgcacggaaa aagatgacga
     5101 agatgagttg acgccggggc gcatcctcgt cgccttcgag aaacttttcg gcggcgggcg
     5161 cggcgtgcaa aagttcgcgc gttccggcct gcgctacatg cggctcgcgg gcggtgcggt
     5221 gctcgtcgaa cagaacccga agaagaagag cgaatgggct aagcttgcac aggcaggccg
     5281 acgcgtcgca tgggtcatgc acgaaggcga atatctcgcg cgcgtcgttg acggcgaagt
     5341 ctccatgctc gattaaagac ggggcacgga gcctcgcgca aagttctcac aacacggaag
     5401 tctcctcgat gagccgcccc tgcgcgaatc tctccgcgcc gagcgcgctc acgtccatca
     5461 actccgcgcg cccgtcgaga atcaactccg cgaggatgcg acccgtcgcg ggcgcgtgca
     5521 tcacgccatg accgctgaac ccgttggcga ggaacagccc gcagacgccc ggcgcttccc
     5581 ctataatcgc gtgatggtcc ggcgtcaact cgtatagccc cgcacgacag cgactctcat
     5641 tcagcgtcgc cccatgcacg agatgcggca cgcgctcgcg tgcgcgctgc aagactttcg
     5701 cggtgaagag agggtcaaag tccgtcttga aacccggcgt ctccgtctcg tcaggccacg
     5761 ccagcagaac gcccggcgac tccaactcgc gcgcttccgg tcggaagtga aacccgttcg
     5821 acatgtctat gaccatcggc aagccttccg gcacaggcgc ggcaggccga acgctcgcca
     5881 actgcctgcg caaaggcacg accggaatct ccacgcccgc catgcgcgcc gtctccgccg
     5941 cccacgcgcc cgacgcattc accacggcgc gcgtcgagat gcgcccgcgc gtcgtctgca
     6001 cgcccgcgac gcgcccgcct tcaacttcga tgcccgtcac ttctgtctcc gtctgcacgc
     6061 gaacgccgcg cgccgtcgcg cgcgcggtga acccgccgag cactctgagc ggcgcgatga
     6121 aaccgtccgt ctggcagaac gatccgcccg tcacgtcgtc cgtacgcaat cccggcacgc
     6181 gccgcgcgat ctcgtccgcg ccgacgagtt cgacgttctt taagccttcc gccagttgtc
     6241 tctcacgggt ccgctgcaag tagtcgaact gctccgcgct ggtcgcgacg aaaagataac
     6301 cggcggcctc gtagccgcag gcgtgcccgg tcgtttcctc gaagcgcgtg aagaaatcta
     6361 tcgagtaacg cgacatacgg atattgatcg cggtcgcaaa ctgcgcgcgc acgccgcccg
     6421 tcgccttgcc cgtcgaaccc aagccctgcg ccgcctcgcg ctcgacgacg acgacatccg
     6481 tgcagccgcg ctccgccagg tgaaacgcga cgctcgcccc gacgatgccg ccgccgataa
     6541 tgaccacgtc cgccgtctgt tgcataggtt tatggaacga agacgatctc gctcacgttc
     6601 ccctgcggca aagcgaagcg gaggcgtcgc tcgtcgcgct cgaaggagac gtttctggcc
     6661 gcatcgcgcc cgtcgatgcg aatctctttc ggtctggttt cgaggccgag cacttcgacg
     6721 gaagtgatgg ggcgcgcgcc gccgaagtcg ccgacgcgtt tgaagcgaat cgcgcccgcg
     6781 ctctgcgtga cggtcgtggc gcgccatgcg ctgcgccggt agccgtagcc gtcgccctcg
     6841 tcttcgtgga tgcgacccac gccgtctgcg ccggagatga tcttgacgga gagcggcgca
     6901 cgcgccatct cgcccgtgtg ctggacgacg gcctgcatgg gaatgatcgc gcccgcccgt
     6961 gcgaagagcg ggaggcgcgc gagcggcgca tcaatctccg ccgtggtgcc gccttcgtag
     7021 cgtgcgcccg tccaccagtc gatccacgcg tcgcctttgg gaaaatagac cggacgcttc
     7081 gtttcaccct gcacgacgac gggcgcgacg agcaggtcgt cgcctaagag aaattgttcg
     7141 gcggggagcg cgggcgcgta ggtgttgtag tcagccggat agttgaacca gaggggacgc
     7201 ataacgggca tcccggtgcg ttcgtgttcg cggaagagcg tgtagatgta agggaggagt
     7261 tgatagcgca actcgattga ggcacggttg atccgctcgt gagtttcacc gaacgaccac
     7321 ggttcgcgct cctgcaagcg cgcagagatg tgggagcgat agaagggtgt gagcgcggcg
     7381 gcctgcaacc agcgcgtgta gagttcggcg gatgcgttgc cggtgaagcc gcccacgtcc
     7441 gcgccgacga agggcacgcc ggagatgctc aggttcgtga gcatcgcgat ggagagttgc
     7501 aggtgttccc aactcggcac gttgtcgccg gtccagacgg cggagaagcg ttgcactccg
     7561 gcgtagcccg cgcgcgtcag gacgaacggg cgcgcctcgg ggcgcaggcg ttgcacgccc
     7621 tcgaaagtcg agcgcgccat ctgcatgccg tagacgttgt gataacgcgc gtggtcgccc
     7681 ggttgcccgt cgcccgcgtg gcgcacgtcg agcgggaagg tcttggccgg gtcgtgcatc
     7741 acgttcggct gcggcgggcg cgtgtcgggc gggaaggtgg cgggttcgtt catgtcgttc
     7801 cagaagccgc tcacgccttc gtccagatgc ttcgcgtaga gcgagccgaa ccaggagcgc
     7861 gcctgcgcgc tcgtgaagtc ggggaaggcg cagacgcccg gccagacgtt ggcgcgaaac
     7921 tccgcgccgt ccggcttccg gtgaaagaaa ccgcccgcgc gtcccgcctg ataagcgaaa
     7981 tagttttcgt caaccttgat gccgggatca atgatgagga cggtacgaaa gccgtcccgg
     8041 cgtaagtccg agagcatctt cggcgggtcg ggaaaccgct ccttgtccca cgtgaagacg
     8101 cggtagccgt ccatgtaatc aatgtcgaga tagatcacgt cggcgggaat tttgcgttca
     8161 cgaaagccgc gcgcgatctc gcgcaccttc gcttcgggaa agtaagacca gcgcgactgt
     8221 tgatagccga gcgaccagag cggcggcaga ggcatgcgcc cggtgagttc cgtgtagtcg
     8281 gcgagaactt tctgcggcgt gcgctcgcgc ccgcccgtga agacgtaata gttcaactcg
     8341 ccgcccgccg cgccgaacgt gtaacgctcg ggcgcgctgt gtcccatgtc gaagtgcgtg
     8401 cggtaagtgt tgtcgaaaaa gagtccgtag gcgcgaccct gccggagcgc gatgaagaag
     8461 ggaatggatt gatagatggg atcgaggccg ggcgtataac caaaggcgtc cgtgttccag
     8521 ttgacgattg actgaccctg ccgcgaagtg ggcagagcct tctcgccgaa gccgtaatag
     8581 aactccaacg cgtcgcgccg cttcgacgcc tcaatcgcgc cgctctcaag atcgaacgag
     8641 acaggacgcc cgcgttcgtc ctccaacaac acgttcccgc ccgcgtccag aatcgtcagc
     8701 gcaaacgggc gacgacgaat aacgatcttc cctccatcca cgcgcgtcgt gatctcaatc
     8761 gtctcacgcg tctccttgat cgacgccgcg accgtcttcc gatcccgcgc ccacgtcgca
     8821 taagagaaat cacgctcgaa cacgccgcgc ggcgcgaacc ggacgcgcac cacgtcgaga
     8881 tcgaaaaacg tcacacgcgc acgcgcgccg ctcgacaacg tgagcacgac gccgttcgtc
     8941 tctcgctcca ccgcgacgac atcgcccgcc gcgcgccacg ccccacgcgc ctcgaacgcc
     9001 gcgaaactaa aagcaaaaag gagcgcacag gccaccgcac gaagttgccg gttatgatga
     9061 agagttgcgt tcatactatt caagtctcaa attcagtgac gagtgacgag ttaaaaactg
     9121 agaactgaaa acttgtcttg atcctttctc gtcacttgtc acccgtcact attgattggg
     9181 acttaactat ttctcataga cgacgcgccc gccgagaatg gtgatggcgg cttcggtctg
     9241 caaaatctct ccgggcttga tggtcaacag gtcgcgcgag tggacgacga ggtctgcgag
     9301 catgccgggc gcgagttggc ctttctgctc ctcttcaaag gacgcgtagg ccgacccggc
     9361 ggtgtagcaa cggatggcct cggcgatgga taagcgttcc tgcggccacc agccgcccgc
     9421 cgggtcgccc tccgtcgagc ggcgcgtgac ggcggcgtag agtccgagat aaggattgat
     9481 cggctcgacc ggccagtccg tcccgaaggc gacgtggacg ccgtgcgcct gcatcttgtg
     9541 ccacgcatac gcgccctgca cgcgatactc gccgaggcga tcctgcgccc aacgcttgtc
     9601 cgagatggcg tgcgacggtt gcatcgaagc gacgacgccg agcgagcgaa agcgcgcgaa
     9661 gtccgacggc gcgacgacct gcgagtgctc tatgcgatgg cgaaatttat taagtccggc
     9721 ttcattctca atgtcagcgc gggctgcttg cgtatcgccc ggttttgcgg gattcgcctg
     9781 cctgacagca ggttcaggcg aaggagcttg cgcaggcttt tcatctcgaa tagagcgtgc
     9841 cgctttaaag ccgtcgagcg ccatgcggtt cgcgcggtcg ccgatggcat gaagcgcaat
     9901 ctggaatccg gcggcatcgc gttcgacgat catgcgcgtc agttcttcgg gcgggcggcg
     9961 cgggatgccg ctgttgtgcg ggtcgtctgc gaagggcgcg agcatggcgg cggtgcgcga
    10021 gccgagcgtg ccgtcaacgt agcccttgag tgcggtcagg cggatgcgca gcgggtctat
    10081 tttgaacgac gcaaactccg cgcgctgggc tttcagacgc tcgaccgtat cctcgaagtt
    10141 ctgccactct gcgacgcgga cggtcagttt tcccgcttgc aggagttcgc gataaagttc
    10201 gattgattcg tagccggagt tgtcctgaat cgaagtgatg ccgtagcggc gcgcctctgc
    10261 gagcgcgcgt tcggcggcct gcatccgttc ggcgcgcgac ggttgcggca cgacgcgccc
    10321 gacgagtccc gccgcagtct ctttcaaaat cccggtcggt tgcccgtcct gatcgcgcag
    10381 aatctcgccg ccttcgggca cgggcgtttc gcgcgtcacg cctgcctgtt tcagcgcgag
    10441 cgaattcgcc caactcacgt ggccgtccac gcgctgcacg tagacagggt tgttcggcgc
    10501 aaccgcatcc aggtcggctt tcgtcggcca ctggccgccc cacagcgtat ggtcccaacc
    10561 gcgcccgaga atccacgccc ccgcaggcag ttcgcgcacc ttcgccgcga tgcgcgcctt
    10621 cgcttcctca agcgtccgcg cgccgacgag ttcgacgcgc aacagggaga ggccgccgcc
    10681 gatgaaatgc aggtgggcat cgttgaagcc gggcgtgacg agacgcccgc gcaggtcaat
    10741 cgttcgcgcg cccctgtaag cgcgattgat ctcggcgttc gtgccgacgg cgacgatgcg
    10801 atccgcttcg atggcgaccg cctccgcgag cgtgccgcgc gcatcggcgg taaaaatctt
    10861 gccgttcgtg agtacgagac taacagcgcg ttgcggcggc agcgttgacg acgcattgaa
    10921 ggcggcgcgc gttgagtcgt gtgtcgagca gagaagcagt gcgacgagtg agagggcgag
    10981 cgtggtgcga agtagtttca taacgggcga ttcagatgtg catgtgaacg tgtccgagcg
    11041 gcgattgtta tatcactaaa cctgcgccgc gcccattctc cggttgggcg tctgcccttg
    11101 tcaggccatg tggagccact acatgccgga gagataattt tcaagcgcac tgttcgggta
    11161 gaggcttatg cggctgcgtt tgtccctgtc atataagttg acggagagac agggcttggg
    11221 agtcacgtct tttaaacttc tccgcacctg cccgtcctgt tgcccgcctg agttggctca
    11281 agccggagcg ttcttaaagt taagcaaaaa tagcgcgcgt gctattatcg agcggacttt
    11341 tcaactttta agggataagg gaagaacagt ttcgcctgtc ggtgagggcg actgttcgac
    11401 agctacatca tgcgcgttct ggtaacgggt ggcgcgggtt acatcggcag cgtcgtgacg
    11461 gaagagttgg tgcgagacgg ccatgaggtc gtcgtctatg acagtctgta taaaggtcat
    11521 cgcggggcgg tggtcgaggg cgcgacgctc atcgaagcgg atttaatgga cggcgaacgc
    11581 ttgcgccgcg ctttccgtga gcagcgcacg gaggctgtga ttcatctggc ggcggattcg
    11641 ctcgtcggcg agtcggttat gaacccggcg aagtattatc gcaacaacgt cgtcgcaggg
    11701 cttgccctgc tcgatgcgat gaacgagtgt gaagtgcgcc gcctcgtctt ctcttcaacc
    11761 gccgccacct acggcgaacc ggagaagcag ccgattgaag agagcgaccc gacgaacccg
    11821 accaacactt acggccagac gaaactggct ttcgagcacg cgctgccgtg gtatgaatgc
    11881 gcgcacggaa tgcattacgc ctctctgcgt tacttcaacg cggcgggcgc aaccgagcac
    11941 tgcggcgaga tgcacgaccc ggagacgcat ctcatcccga tcattctgca agcggcgaag
    12001 ggcacgcgcg aggccgtgga aatctacggc gaagactacc cgacgcgcga cggcacctgc
    12061 gtgcgcgatt acatacacgt cgtcgatctc gcacgcgcgc acatcctcgc gctcggcata
    12121 ctcgacgagc gcagcgcgat ctacaatctc ggctgcggcg gcgacggcta cacggtcagg
    12181 gaagtgatcg acacggcgcg caaggtgacg ggacgcgaga tcaaagtgcg cgtcggcgca
    12241 cggcgcgcgg gcgacccggc cttcctcgtg gcgagttccg cgcgcatcaa acgcgaactc
    12301 ggctggtcgc cgcagtttca ggatttgcac ctcatcatcg agtcggcctg ggcgtggatg
    12361 gagcggcacc cgcgcggcta tgaggaatag aggcgacgct tcgcgcgcac cggcactcga
    12421 accgggcggg gaagagcggg cgggaaaaaa ggcgggcggc gcacagtcga aatactgcgc
    12481 gccgcccgct ctgtttatgg gttggaacga aggcgcgcaa tccgtgcgtg cgcgccttcg
    12541 tagagtttaa cgcttacgct tcgcgccctg tcgggtcgct gccgccgcgc ccggcttggc
    12601 agacttcttc gtggcgggag tcttcatcgc ggcagcggct tcgctcgtgg aggctgaggg
    12661 cgtgatcacg gcttcgacgt tgacgaggaa gcggaagaag ccgttttgca tcacgccgag
    12721 gttgaactcc acgttgaccg atgcgcccga agcgatctgc acgggcgcga tgaattcgac
    12781 ggttgaattc acgccgccgc cgagcggttg attcggcggt tgccccgcct gcgtgcccga
    12841 cacaggcacg accgctccgg tgctgagcgt gacgttgaag ttggaagcgt ccgaaggcac
    12901 gacgcgcagg tcggcctgcc cgttgcccgg agcatagccc ggactgttga gcgtcgtgat
    12961 gtctacgacg cggaagcgca ggcgcgtcac gatgccgccc gtagtgttgg tgaacttccg
    13021 gcgcagcttc aacgtgccga agtctccgtt gggcacgggc gtgccgatgc ggcggcgatt
    13081 ctcgcaagtg cccgcgcccg cgcaggtgtc tatggacgaa gccttgatca cgctgttgcg
    13141 ttggatgggc gaagcgaggt tctccggacc cggcgcgccg agcacggatt gcacgccgtt
    13201 gaactgcgcg gctgacgtgg agacgaagac gaaatcggcg gcgttgtcgt tcgtgtcctg
    13261 cggcagcccc gtgaccagtt tgcgcgcgaa actatagtcg ccgtccaccg agccgacggg
    13321 cgggagcgcg gtgccctcgt agtagagcgg gctggcggtt gtgaacccga ccgcgtcgaa
    13381 gcgtgtggtg ggcgtaaagg ttgtggacga gccgaagagg gcgatgcccg tgtcgtcttc
    13441 aatctccgtc tcgtaagtca tgttgggggc ggcgacggtg gacagcgcgt agagggcgtg
    13501 cgcgccgaga tagtggctgc gcgcagggat caacgtgccc gccggaatga cgaagcgcac
    13561 gccgccattt ggggcgacga ccgcccaccc ggcggagttg tcggcggtat caaccgtgaa
    13621 cgggacgttc gtgttgttgt agatttccac gaactcgtcg aaaaggccgt cggacactcc
    13681 cggcgggcct tgcccacgga aacgaaactc gctgatgagt atctgtccgg cgacgggcag
    13741 cgggcaggcg ttgcctctgc cgtcgtcgtc cgtgtcggcc tgatcggggt tggcgaggta
    13801 gcggcagttg tcgctgccgt cgggcacgcc gtcgttatcg tcgtcggggt cgcaggcgtt
    13861 gccctgcccg tcgccgtccg tgtcggtctg gtcctggtta gccacggtcg ggcagttgtc
    13921 ggaggtgtcg gggatgccgt cgtcgtcatc gtcggtatcg cagccgtcgg gcgcgccgtc
    13981 gccgtccgag tccgggagcg gggcggtcag gaacgggatg aagtcaacca cgaaattggg
    14041 atcgcggatg acgacgcccg tgcccgcggg gttgcgcccc tgctcgtcgg tggggccgtt
    14101 cggtgcgccc cagtagttgt tctcggcgtt gagcgtgccg gtgtattgcc ccgtgtcgat
    14161 gcgaatgccg aagggcatcg tctcgatgtt gttgaagttg gcggtgacgt tggagttcgt
    14221 gccggagccg aagttgggga tgcggatcgc gctcgtgttg gccgcgccgc cggagatcgc
    14281 gttgcaggtg atgacgatgc cgttgttgcc gccgccgatg aagatgacgc tgccctgcga
    14341 acccgtcacg acgttgcccg tgatgatgat ggcgctcgaa ttgaagaaga ccatcgagtt
    14401 gtcgttgacg agttgattcg aagagacggt gatgttgctc tgcgtcccgg cgaagaccat
    14461 cgccgcagag gtgtgcccgg taaagcgatt ttgattgatg acgacgttcg acgcgccctg
    14521 atcggaatag atggcgttgc cgctcgccgc gcccgcgcgg ttgttgccct cgaagcggtt
    14581 gaaacgcacg atagactgga tcgcgccgtt gctgttgagg tagaggccga aggtgttgtc
    14641 gcggatgatg ttgttgaaaa tccggtggcc ggagcgattg gccgcgagcg tgatgccatt
    14701 ggcgaggggc gcggcgctgc cctcctgcac ggtgaagccg tcgaccgtgg cgttcgaggc
    14761 ggtgatgttg aaagagccgc cgggagagtt cagcacagac tcgtccgtga ccgggacggc
    14821 gcgcgtgcgc gcgtcaactc cggcctgcgc gccgcgcagg ttgagagcct tgttgacggt
    14881 gacagtctcc gtgtaagtcc cggcgcagac atcgatctga tcgccgttgc tggcggctgc
    14941 taccgccgcc gtgatgctgg tgtgcgcggc aaaggggcat tgcacgcggt cgtcgtcaac
    15001 ctgccggatg cgcccgcccg aaggcgattg gcaagcatcg ccgatgccgt tctggtcttc
    15061 gtcttcctga ccggggttcg gggtgttcgg gcagttgtcg gcgccgtcgg gcacgccgtc
    15121 gaggtcgagg tcgggttcga agttgaaggt cgtgtaggcg tcggcgatgc cgatggtgaa
    15181 ggcgtccacg ttgccgtcgt agccgttagc ccaggggccg cccgcgcgga agaggagcac
    15241 gcccgccgtc tggggcgtgc cgccgtggaa gatggtggcg ttcgggaaac tggcgagcac
    15301 ctgcggccag gtgcaggggg cgttctgcgg gcagacgccg ttgctcccgt ttgcgcccgg
    15361 cctcgacgca taccacctac ccgccagcgc atgccacgtc tgccagacgt tctgctgcgg
    15421 gttggtgccg ctttgatagg gttcgaatac gagccgaccc tgataggcgg cgttgccgcc
    15481 cgcgccgtca tagtcgatgt cgaactggag ggcgatgtcc accgcctgat ccggattggt
    15541 gttcttataa gtgctgtagc ggagttgaga aatttggtcg aggcgcgttc cggcgtaggc
    15601 ttgggtgccg aggttgtggc ggcctgtcgc atcgacggtc aggttcgcgc tgccgccgcc
    15661 gagcgggggc gtggccggac cttgctcgaa cccccccgaa ccgttcgcgc cttcgttgaa
    15721 gaagaaccag ccgttgagat ttgcgggcaa cacgagcacg gcggtcgagt ccgggtcgcc
    15781 gtcggcgtcc gcatcgtttg cggagttgag cagcatgtta tcgatcagga agccctgccc
    15841 ggcggtggcg ggagcgggcg cgccgcttgt gcggaagaga attgaatcga ccgtgcgggt
    15901 gggattttct tcaacgtcgc ggtagtagtc ttcccaactc gtgcccgtgt gttcgagcac
    15961 gccgttgaca taaactctga cgacatcgtt cgagtcgccg tcgacgaagt tcatcgtgag
    16021 cttgatggtg tgcggcacgg cgcggctgag gccgctcgtg aggttggtct gcacgaagtc
    16081 gttctggcct cgctggtagt cgaagaaatt aacttcgagg ccggagggcg tgtcgagcat
    16141 ctgcacccaa ctcatgcgcg cgccgtcgcc acggtcgggg ctggcgacga cggagaggcc
    16201 gggctgctcc gagccgggga cggtggaggc aaattgccac tcggcctcga agtagttctg
    16261 gcgcacgcct ctggataaac cggcgttctc cgcgcccgtc tcgcccgcct cgttgacgac
    16321 ggatttcgag aaggtgccat cctgaaagca gccgctcgtc accgcattcg acatgcgcaa
    16381 actctgcgtc ccgaaagcgg cgggcgcgcc cgagttggag acgaccgcgt gatcgtattg
    16441 agcgcagccc agacccgcgc tgcccagact gctccaaccg tcttgattat gaattgagcc
    16501 gggggcataa gcggtcggct cgaacctgat ggctgaactg tcggctgtga ccacgaccag
    16561 agggagaatg agaacggcaa gcagcgtgag tacgacgagg gaaggcagaa tttttctgta
    16621 gttcattcgg gggaatactc ctagagagag agtgcgcact gagtatagac agcgggttac
    16681 ggcctaaccc gtcgaggata ctctgacagt ctgctataca gaggcgggct atatagtgtc
    16741 taaaaggcta ccaagagggg cagccgggct ttgatgaggc aggataatag tcgctggctt
    16801 aactgcaagt caacttcttt tggccttttc gggcggaaat tcagaggccg gaagtcagaa
    16861 aatcctgatg cgtgaaagca gttttctact gtctctcaac gcagcggttt tcacttccca
    16921 ctgcgtcagg aagtctcgcc ctccgccgtt agaaactggt tcaactcgtg ggcggtgggg
    16981 agggcggcgc gtgcgccgag gtcgcggcat ttgagggcgg cgacggcgca gccggtgttg
    17041 aggcaggctt cgattgtttc gccttgcagc atcgcataga ggaagcccgc gtggaaggcg
    17101 tcgcccgcgc cggtcgtgtc gcggcagccg ccggggacgg ggaaggagtc cgcttcgtgg
    17161 tacgcgcctt cgtggtaaac aagcgcgccg cgcacgccga gcgtcatgcc gacaacggcc
    17221 ttttcgtgca ggcgcgcctt cacttctttg agcgcggcgc gttcgttcgg gatgcccgtc
    17281 aggcggcggg gaaactccga cgaggtgatg agcacgtcga ggagcgggag aagttcgggc
    17341 agtccttcgt agatgttgtc gatgtcggcg gagacgatgg tgcccgccgc acgcgcttcc
    17401 cgcgcgagcc gcgcacaggc cggaggatcg tgagcgtcca gatgcagcac gcgcccgcgc
    17461 gccgcgaacc ctgcgggcgc atcctccgcc gcgtatgcca accgcgcatc acgctcccac
    17521 aggatcgtgc gctcgcccgt ccccgcgtcg atgatgatga acgcgatctg gttacgcgcg
    17581 ccctcgacct gctgcgcgtg ctcgacattg acgccttcag ccacgagcgt cgccagcccg
    17641 aactcgcctt ccgcgtccga cccgaagcgt ccggcgtaag ccgtcggcag gccgagacgc
    17701 ttgagggcga ccatcgccga agcggtctgc ccgcctgccg cctgcacgtg cgtcttgaaa
    17761 cgcgtcttcg tgtcgaaggc cgggtaggcg tctacgacga tcaagtgatc gaccgcgttg
    17821 aggccgaaac cgacggcctc gaattctctc ccggcgggca ggttaaaggg gaagcgcacg
    17881 gacgttgaca gcctttcgcg ttgctcaaac tcggctcgcc atagtgccag cggggagggc
    17941 gtttaagcaa actacaaacg cgtgtcaaaa ctacttcccg ttttaacttg cgcgccttct
    18001 cgcgtctcta tcacgtttac gttatagtaa atgcgacccc ccgaaatctt tagaggcagc
    18061 gttcaccggc agtgactctc gaagagaaac tcaaaaatct gcccctctcg cccggcgtct
    18121 atatacacaa ggacgaggcg ggcaagatca tctacatcgg caaggcgaag aacctgcgca
    18181 atcgcgtgcg ctcctatttt cagtcggggc gcgggcacga ccgcaagacg cgcgaactcg
    18241 tccggcgcat acgcgatctc gaattcatcg tcaccgacac ggaggtcgaa gctctcgtcc
    18301 tggaatcgaa cctcatcaag aagcacaagc cgcgttacaa cgtccagctc aaagacgaca
    18361 agcaataccc gcatctgaaa ctgaccatca acgaaccgtt cccgcgcgtc atgatcacgc
    18421 gccgcgtgca gcgcgacggc gcgctctact tcggaccctt ccttcccgca tccctcgcgc
    18481 gccgcacgat tgatctcatc aaccgcacgt ttcaactgcg cacctgcgac atcgagattg
    18541 acggcaaact cccgcgcccc tgtctcgaat atcacatcaa acgctgtctc ggtccctgcg
    18601 tgcgcggcct gtgcacgccc gaagagtatg ccgagtcggt gcgcgacgtg cgcctgtttc
    18661 tcgaaggcaa agacaagcaa ctcgcagacg aatacgcggg gcgcatggag cgcgcgagcg
    18721 aggagatgcg ctacgaactc gccgccaagt atcgagactt gcgcaaaacc gtgctcgccg
    18781 tctccgagac gcagaagatg gcgacctcgc ccgagcgcga cgtggacatc ttcggctatt
    18841 accgcgaagc gcagcggctc gccctccaac tcttcaccat gcgcgaaggc aagatcgtcg
    18901 gtcgccgcga gttcttctgg gaagacctgt cggcggacga gttcgacccg gcggagttct
    18961 taagcgaagt tctgacgcaa tactactcga ccgattatgt gccgcgcgaa attcacgtcc
    19021 ccgttgattt cgaggatcgc gaactgttgg aacgcgcgct ctcggagcgg cgcgggcggc
    19081 gcgtgcgcat actcgatccg cagcgcggcg agaagcgaga catgatcgcg ctcgtcgaga
    19141 agaacgcgca gatcgctttc gagcaacgct tccgcgtgct caaacccgac atggaacgcg
    19201 tgctcgaaga gttgcaggag acgctcgaac tgccgcgttt tccggcgcgc atcgagtcgt
    19261 tcgacatctc gaacatttcg ggcgcggaga acgtggccgc catggtcgtc tgcgaaaacg
    19321 gcaagatgaa tcgtgccgaa ggacgccgct tcaagatcaa gacggtggaa ggttcgaacg
    19381 acgtggcctc gatgcgcgaa gcggtcgcgc gccgctaccg caggcagttg gacgagaaca
    19441 acccgctgcc cgatctcgtg atgatcgacg gcggcaaagg gcaactcaac gccgccgccg
    19501 atgcgatgcg cgaactcgac ctcgaagcca ttccgatgat cggcgtcgtg aagccgccgc
    19561 gccgtcacaa cgaagtctcg cacctgctgg tcaaagggcg cgagcacgaa cccgtctatc
    19621 tcgattcgca ttcggcggtc ttgcgtctca ttcaaatgat ccgcgacgag acgcaccgcg
    19681 ccgccgtcac ctatcaccgc aagcggcgcg agatacgcga cttcacttcg gaactcaccg
    19741 agatacccgg cgtcggcgac aaacgcaaga accgtctgct tagaaacttc ggctccatcg
    19801 cacgcatcgc gcaggcgacg gcggcggaac tcgcgccctt catcggacgc cagaccgccg
    19861 aagaggtcgc cgcgcacttc acacgtcaac gcgcgcttgc cggaggcgac aacaacaacg
    19921 gcggcgacga cgcgggcgcg gaaagcgtcg cgccgtcgct tgccacgacc ggcagcgtcg
    19981 gcgcaaggga tacggacgat gcagacgaga tgccgctcga agttgagacg cggcttgacg
    20041 acccggaagg cgacgcggaa gacttgcaac ccatacgctc cgtagatcac gcgggcgagg
    20101 tgcgcccccc gcgccgcaag tccacgcgca agcggggcga gcgttcgaca aacccgcacg
    20161 gcatcaagcg cgagcgcatc ggtaaaagcg aaaccgagta ggcagccgaa ttgatagccg
    20221 ggtaggaagt tgagttacat gaacccgccg acgcacatcg aaaccgcgcg gctcattctg
    20281 cgcaagcctg tcctgaccga cacgccgctc atctacgagg cttatgcgag agacgcagag
    20341 gtgacgcgct acctgacctg gcgaccgcat cgcacgctcg cggacggcga cgaaccggcg
    20401 gcgcaacgtc tggccgcgtg ggaggcgggg agatattttt cgtgggtcat cattctgcgg
    20461 agcggaggag agttggcggg gatgatctct gcgcgcccgg acgggtggcg cgtcgatctc
    20521 ggttacgtgc tggggcgcag gtggtggggg cagggttaca tgacggaagc ggtgcgcgcc
    20581 gtgagcgatt gggcgttgtc cgagcgcgag gtgtttcgcg tgtgggctgt gtgcgacgtg
    20641 gataatccgg cgtcggcgcg cgtgttggag aaggcgggga tgcggcttga gggacgcttg
    20701 cggcgttggg cgttgcaccc gaacatgagc gacgagccgc gcgattgtct ctgctacgcg
    20761 cgagtcaaag aagacgacgg ctcacggtcg tcataacaaa gttcattggc acgattcatt
    20821 actacacaat tcattaccgg gaggaatgag cgatgaaaac tgataacggg cagacaactt
    20881 cggtctggat gcagacggcg gacgtgcctg aggggctgcc actcggcgcg gacgcgcagg
    20941 ccgacgtggt gatcgtcggc gcaggcatcg cgggcttgac gacggcttac ctgctgacga
    21001 aggaaggcaa gcgcgtcgtc gttctcgatg atggacccat cgccgggggc gagacgtgcc
    21061 gcaccacggc gcatctctcg aacgcgctcg atgatcgtta ttacgaactc gaacgcctgc
    21121 acggcgagga tggcgcgcgg gttgccgccg agagccacac ggcggcgatt gatcagatcg
    21181 aagcgattgt taatgaggaa aagatcagtt gcgacttcgc gcgcgttgac ggttacctgt
    21241 tcgttccgcc cggcgacgac ccggcgcaac tcgacgacga actcaaagcc gcgcaccgcg
    21301 ccggtttgac cgaagtggaa cgcgtcgaac gcgcgcccct cgattcgttc gacacgggga
    21361 ctgcgctgcg ctttccgcga caggggcagt ttcacatcct gaaatatcta aacggcgtgg
    21421 ccgcagccgt gcggcgtagc ggcagcgaga tctacacgaa gacgcacgcg atcaagatcg
    21481 aaggcggcga ggagggggcg cgcgtcgaga cgacgaacgg cgcgtccgtc acggcgaagg
    21541 ccgttgtcgt ggcgaccaac tcgcctgtca acgaccgcgt cacgatgcac acgaagcagg
    21601 ctccgtatcg caccttcgtc gtcggcgcgc gcgtgccgcg cggcagcgtg ccgcagattt
    21661 tgttgtggga cacgcccgac ccgtatcact acgtgcgctt gcagcgcggg agtgacggta
    21721 aaggcgaaaa cgagggcggc gcgtacgacg tgctcatcgt cgggggcgaa gaccacaaga
    21781 cggggcaggc ggacgatgcg gaggcgcgtt tcgcctgtct cgaagagtgg acgcgcgagc
    21841 gttttccgca agtcgagagt tttgagtttc gctggtcggg gcaggtgatg gagactgtgg
    21901 acggcctcgc cttcatcggt cggaatccgc tcgacagcga caacgtctat atcgccacgg
    21961 gcgactccgg ccacggcatg acgcacggca cgattgccgg aatgcttttg accgatctca
    22021 tcctcgggcg cgaaaacagg tgggcggaac tctacgatcc ttcgcgcaag ccgctgagcc
    22081 tgagcgcggc caaagatttc gccggggaga acctgaacgt cgccgcgcag tacacagacc
    22141 acgtgacgcc gggcgaggtg tcggatgagt ccgagatcaa gtcgggcgag ggggcgatca
    22201 tccggcgggg cttaacgaaa gtggcctgct atcgagacga atcgggcgcg ctccacgagc
    22261 gttcggccat ctgcacgcac ctcggctgcg tcgtgcagtg gaactctacg gagaagactt
    22321 tcgactgtcc ctgccacggc tcgcgcttcc agaaggacgg ccacgtcgtc aatggtcccg
    22381 ccatcagcgc gctggccgaa gtggagaaaa gctgagccgg aaacgatgaa cgagaaacga
    22441 tgaaggatga acaatgaatg acgagcagga gtcagaattc agaatacagg agtcggaaga
    22501 gaacatgcgt gggtcttcat tctgaattct gaattctgaa ttcggactcc tgttttccgg
    22561 attctctttc tgcgtccatc gttcctcctt catcgttgtt caaggtcgta gccggacgcg
    22621 atagaggatg tagttgccga tgcgttcgcc cgcgtcgcgt gtgtaccagc ggtcgagatt
    22681 ggtcaggtcg ctttggcctg cggtggcgac gacataagcg agggggagtt gcgcggcgcg
    22741 ctcgcgtgtg aagttggaga tgtatccggc gtaggcgctc cgttcggttt cgatctctgc
    22801 gggcgtgacg ggcgcgcggt tgggactgag gccggagagc gtgcgacccc agccgaagat
    22861 cgcgtggtag tagagactcg tgcgggagat gaaggcgtga aacttctgct cgtcaac
//
LOCUS       DASWNS010000380         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_403660, whole genome shotgun sequence.
ACCESSION   DASWNS010000380 DASWNS010000000
VERSION     DASWNS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403660"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VGB76_13410"
     CDS             <1..305
                     /locus_tag="VGB76_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY0099940.1"
                     /translation="SIRIHRRDVQRLIFKALGMTDETARARFGFFLDALEFGTPPHGG
                     IASGIERTLMVLANTENIRDVMAFPKTASAADLMIDAPGEVDPHMLEDLHIKFAGE"
     gene            333..1700
                     /locus_tag="VGB76_13415"
     CDS             333..1700
                     /locus_tag="VGB76_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0099941.1"
                     /translation="MKKRLSIVQSLALCLSLSLFTVALAQAPAPAAQPKRIAVRAARM
                     LDVRSGSVVTNAVVIVENGRIQSAGSGLSIPAGTEVLDLGGAMLLPGLTDSHTHLLQN
                     YDKRIGGDDPNMILTVTQLGTTRRALLGAAQAREMLEAGFTAVRDLGNSGWNGDVALR
                     DAIRSGWVVGPRLFASTRALSAAGGQFGGVSAEMQKILEQEYVVISGVEEARRAVRQA
                     FYDGADLIKVIVNTGARVVSLEELRVIVEEAHRVNRKVSAHAIGDAATRMAAEAGVDS
                     IEHAYTIPDDALRQMAAKKIFLVPTDFPSEHYLPPNLTPEQRQAALQNIRGFTSGSRE
                     RLARAVKLGVRISAGSDSYYAEPGMTRGQTSKLMFRAYLESGMTPLEIIRAATINNAE
                     LFAGERALFGVIETGKFADLIAVTGDPLKEIKELDNVRFVMTGGAVVKDELTTRGATA
                     GRATK"
     gene            1702..2184
                     /locus_tag="VGB76_13420"
     CDS             1702..2184
                     /locus_tag="VGB76_13420"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEY0099942.1"
                     /translation="MSAEEVHTRQVEGERLIVTLRRSQVEEMLAHAREARPEECCGLV
                     GGDSGRVGASVYRLRNIAPDRRVAYDGDPQDLCEAQRLMRARGEQLLGVYHSHPREAV
                     PAPSETDVRLAYHPSAIYFIIGLGEEARAEEFAGSLGAFRIFRSEKRWEKVEYRIVES
                     "
     gene            2227..2958
                     /locus_tag="VGB76_13425"
     CDS             2227..2958
                     /locus_tag="VGB76_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099943.1"
                     /translation="MSEKRSKYDTDPLDPDFVRQTEEIGGASARETARTTNEQARLNP
                     DADAPTRRIDDPLSQPYPSIFVPPTQTQTPPPPTYQQPTYQPPTYQQPPYTQPIASNT
                     MRGAGATGGVPPVPNRGQPTSRPVTGINIPEKFALILPYVPFYIGIVGAVIELLLVPR
                     SETRVRFHAAQGLALQLAILAIGFALRILGGLAGSGFGGTLFWLAANIFLIVSIIRVW
                     KGETHYLTPLDDATSWLHERIEPRK"
     gene            complement(3206..>3507)
                     /locus_tag="VGB76_13430"
     CDS             complement(3206..>3507)
                     /locus_tag="VGB76_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099944.1"
                     /translation="LAELDKPRDGGNGDGVINGDDEVYEKLRLWQDANHNGISEAAEL
                     HSLLELGVRKIELKYKESKRTDEHGNRFRYRAKVWRMGEPKASRWAWDVFLVPSF"
BASE COUNT          666 a         1134 c         1105 g          602 t
ORIGIN      
        1 gctcgattcg tattcaccgg cgcgacgtgc agcgtctcat cttcaaggct ctgggaatga
       61 cggacgagac ggcgcgcgcg cgtttcggct tctttctcga cgcgcttgag ttcggcacgc
      121 cgccgcacgg cggcatcgcc tccggcatcg aacgcacctt gatggtctta gccaacacgg
      181 agaacatccg cgacgtgatg gcctttccca agacggcttc ggcggctgac ctgatgatcg
      241 acgcgcccgg cgaagttgac ccgcacatgc tcgaagattt gcacatcaag tttgcgggtg
      301 aataacgcgc gccggagatg gtagagacga agatgaaaaa gagactctcg atagttcagt
      361 cgctcgcgtt gtgtctgagt ttgtcgctct tcacagtcgc gctcgcgcag gctccggctc
      421 ccgccgcgca gccgaagcgc atcgccgtgc gcgcggcgcg gatgctcgac gtgcggagcg
      481 gtagcgtggt gacgaacgcg gtcgtgatcg tggagaacgg gcgcatccag tcggcgggca
      541 gcggcctctc gatccccgcc gggacggaag tgctcgacct cggcggcgcg atgctgttgc
      601 ccggactcac ggacagccac acgcacctgc tacagaacta cgacaagagg atcggcggcg
      661 acgacccgaa catgatcttg acggtgacgc aactcggcac gacgcgtcgc gcgctgctcg
      721 gcgcagcgca ggcgcgcgag atgttggagg caggttttac ggcggtgcgt gatctgggca
      781 actccggttg gaacggcgat gtcgcgttgc gtgatgcgat tcgttcgggt tgggtcgtgg
      841 ggcctcgcct cttcgcttcc acgcgtgcgc tgtcggcggc gggcgggcag ttcggcggcg
      901 tctcggcgga gatgcagaag atactcgaac aggagtatgt cgtcatcagc ggcgtcgaag
      961 aagcgcgccg cgccgtgcgg caggcttttt acgacggcgc ggatttgatc aaggtgatcg
     1021 tcaacacggg agcgcgcgtg gtgtcgctcg aagagttgag agtgatcgtc gaagaggcgc
     1081 atcgcgtgaa tcgcaaagtg tccgcgcatg ccatcggcga tgcggcgacg cgcatggccg
     1141 ccgaagcggg cgtggattcc atcgagcacg cttacaccat ccccgacgac gcgctccggc
     1201 agatggccgc gaagaagatc tttctcgtcc cgacagactt cccctcggaa cactacctgc
     1261 cgccgaacct gacgccggag cagaggcaag ccgcgctgca aaacatcaga ggcttcacga
     1321 gcggcagccg cgagcgtctg gcgcgcgccg tcaagctggg cgtgcgcatc tccgccggtt
     1381 ccgattccta ctacgccgag ccgggcatga cgcgcgggca gacgagcaaa ctcatgtttc
     1441 gcgcctatct ggaatcgggc atgacgccgc ttgagatcat acgcgccgcg acgatcaata
     1501 acgccgagtt gtttgccgga gagcgcgccc tgttcggcgt aatcgagacg ggcaagttcg
     1561 cagacctgat cgccgtcacg ggcgacccgc tgaaagagat caaggagctt gataacgtgc
     1621 gcttcgtgat gacgggcggc gcggtcgtca aggacgagtt gacgacgcgc ggcgcgacgg
     1681 cggggcgtgc gacgaagtaa agtgtcggca gaagaagtgc atacacggca ggtggaaggg
     1741 gaacggctta tcgtcacgct ccggcggtcg caagttgaag agatgctcgc gcacgcgcgc
     1801 gaagcccgcc cggaagaatg ttgcggcctc gtcggcggcg actcagggcg cgtgggcgcg
     1861 tcagtctatc gcctgcgcaa catcgcgccc gaccgacgcg tcgcctacga cggcgacccg
     1921 caggacttat gcgaggcgca acgcctgatg cgcgcgcgcg gcgagcaact gctgggcgtc
     1981 tatcattcac acccgcgcga agccgtgccc gcgccttcgg agacggacgt gcggctcgcc
     2041 tatcaccctt cggccatcta tttcatcatc ggtctgggcg aagaggcgcg ggcggaagag
     2101 tttgccggga gcctcggcgc gtttcgcatt tttaggagcg aaaagcgttg ggagaaggta
     2161 gaatacagaa tcgtcgagag ttgatttgaa gcgaacctgc gcagcgcatg attcgtaaga
     2221 gttgctatgt ctgagaagag aagtaagtac gatacagacc cgctcgatcc tgacttcgtg
     2281 cgccagaccg aagagatcgg cggcgcgtcc gcgcgcgaga cggcgcgcac cacgaacgag
     2341 caggcgcgtc tcaacccgga cgccgacgcc ccgacgcgtc gcattgacga ccccttatcg
     2401 caaccctacc cgtcaatctt cgtgccgccg actcaaactc aaacgccgcc accgccgact
     2461 tatcaacaac cgacttacca gccgccgacc taccagcaac cgccatacac gcagcccatc
     2521 gcctcgaaca cgatgcgcgg cgcgggggcg accggcggcg tgccgcccgt gcccaatcgc
     2581 gggcagccca cgtcgcgccc cgtgacgggc atcaacatcc ctgagaagtt cgcgctcatt
     2641 ctcccttacg tgcctttcta catcggcatc gtcggcgcag ttatcgaact cctcctcgtg
     2701 ccgcgcagcg aaacgcgcgt gcgcttccac gccgcacagg gcttagccct gcaactcgcc
     2761 atcctcgcaa tcggcttcgc gctcagaatc ctcggcggcc tcgcgggcag cggcttcggc
     2821 ggcacgctct tctggctcgc cgccaacatc tttcttatcg tctccatcat ccgcgtgtgg
     2881 aaaggcgaaa cgcactatct caccccgctc gacgatgcca cctcgtggct ccacgaacgc
     2941 attgagccgc gcaaatagaa gccacaacta aaaggtcgaa cgccagaccg cacccgtcgc
     3001 acgcgcgccg cacaccgccc gacgccagcg aagaacgatc agcagcacac tgccgggtgg
     3061 gcggctgcca gcggtttgcc gggcgcggtg tcgccgttaa cgactgccat gcgagtagag
     3121 acgcttgcga cgcaggcaaa ctgcccgccg atcatcaaga tgatcggcgg gcagcttgtt
     3181 tgtgacgatt gagaggcgcg acgtgtcaga aggacggaac gagaaacacg tcccaagccc
     3241 accgactcgc ctttggctct cccatgcgcc agactttcgc acggtagcgg aagcggttgc
     3301 cgtgctcgtc cgtgcgcttc gactctttat acttcaactc aattttccgc acgccaagct
     3361 ccagcaaact atgcaactcc gccgcctcgg agatgccgtt gtgattcgcg tcctgccaga
     3421 ggcgcaactt ttcgtacact tcatcgtccc cgtttatcac gccgtcgccg ttgcccccgt
     3481 cgcgcggctt gtccaactcg gcgagcg
//
LOCUS       DASWNS010000381         6023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_143767, whole genome shotgun sequence.
ACCESSION   DASWNS010000381 DASWNS010000000
VERSION     DASWNS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143767"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..924)
                     /locus_tag="VGB76_13435"
     CDS             complement(247..924)
                     /locus_tag="VGB76_13435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0099945.1"
                     /translation="MRPLPLRAPTVSLGTFKTLTFLLFLPLMLLCCASTARAVPLVIT
                     GGTATTPIGLNNFNLNVTGLNFSFHGSNTSSGETQLCGLCQPGRQFGGTFPVRIDTIL
                     NLTYNGTTYRPGVGYVVISGDNFITLPLLTVPADYSPIVSPFSFIGRITVDPSDGSAP
                     FTFDLTGAGTATFIFLPVNNGTSSLVQATFAFAPPPEPVPEPATLLLLGTGLAGVVAK
                     VRRRRRQ"
     gene            1155..2519
                     /gene="aroA"
                     /locus_tag="VGB76_13440"
     CDS             1155..2519
                     /gene="aroA"
                     /locus_tag="VGB76_13440"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY0099946.1"
                     /translation="MRIQPARRLRGRVLVPGDKSISHRAAIISALAGTGGRSRLTNYS
                     TSADCAATLDCLRALGVRIERDGNNVQVEGRGTRWTLEEADEPRAPLDCGNSGTTMRL
                     LAGLLAGQPFTSTLTGDASLRSRPMKRIIEPLELMGARISSAAGRAPLRIEARRSPLA
                     PVRYALPVASAQVKSCLLLAALNAGGRSEIIEARGATRDHTERLLSLFGASVQTEATT
                     YEGRTVPVVSLQTPVSLEARDCDIAGDISSAAFFIAAAALLPGSELTLPAVGLNPTRT
                     EILHTLRKLGAEVSVAGESTESGEAVGELRVRGGLAPTTPTAAAATTTADAHVLRGEL
                     IAGLIDELPILCVVGTQIEGGLEIRDASELRVKETDRISAMVKNLRALGAGVEEFADG
                     LRIEGRTPLRGARLESFGDHRIAMACAVAALIAEGESELAGADAVAVSFPEFFGMLES
                     VTER"
     gene            2516..3055
                     /locus_tag="VGB76_13445"
     CDS             2516..3055
                     /locus_tag="VGB76_13445"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY0099947.1"
                     /translation="MKAGARRIVLTGFMCAGKTTVARALAARLDCLMLDTDAVVVERT
                     GRPVEAIFAEDGEARFRQIERKVLRDCLENAAARVIALGGGTWTLAENRALIAAYDCL
                     TIWLDAPFALCWQRITAAVARDETARPLARNHEHALALYTSRRDAYSLAARRVQAGEE
                     HSAEDTATEIMSIIERENF"
     gene            3099..4625
                     /locus_tag="VGB76_13450"
     CDS             3099..4625
                     /locus_tag="VGB76_13450"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEY0099948.1"
                     /translation="MANRDADAKALKVAKKLLNAENWKHLARWHGDGYYELHTEDTGD
                     TPVRLFLTKQLLDDAEDILYRQIVNATRFPGVRMVVITPDTHYGYGVPVGCVLITDAD
                     AGAVAMGPVGYDIGCGMMSARSEVEAERATPEKRLEFNRAVMERVDMGAGGKSVRLGE
                     LSKSEFNNLVRGGAEYYVEKYGATFDRSRAERHRIPVDDDWQIPWGGKGRPERGLNQM
                     GSLGGGNHFIELQRSEQTGTVFVQVHTGSRGFGHGLATNYFEMAREERPEAITDIDLG
                     YFTPDSRHYSSYLNAVAAGGNYAILNRLIIFEQVSDAFREVFGQELELIYEISHNLVQ
                     RETHPEFGEVWVHRKGATRAFPAGHPALAGTHWEATGHPVLIPGSNKDWSYILQPLAG
                     AVNSGYSVNHGAGRRLSRGEASRTLSQRAIDDEYKAAGILVNINGRVPLDEAAPAYKS
                     SQAVIEAVVGAGLARIEHQLWPLASLKGADERKGKRRGKGGKADTGDAKQGRKTKEHY
                     "
     gene            complement(4817..5881)
                     /locus_tag="VGB76_13455"
     CDS             complement(4817..5881)
                     /locus_tag="VGB76_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099949.1"
                     /translation="MLSVQAPLEDKLAIKSAAESVARGARARWWRRRGTKRSGFRYED
                     AAARRITDEAALERIKSLVIPPAWKEVRISPSSRHRLQAIGLDAGGRVQRIYHPAFAA
                     RQARRKYAKIERFGEQLKHLRRRTNEDIAREGLPRERVLAVIVRLINDLYFRVGSEES
                     VKRYRTYGITTLRNRHLEFKRGGRLIFSFIGKHHIKHRRILVDEELATLLRDIQSLRG
                     KRLFQYWDEANRRPVAIKPRDVNEYIKAATAPEFSAKDFRTWGGTLLCAVSLAELGKP
                     ADDKQAKRNILHAIRSVAEHLGNTPTVCRSSYIHPAVIERYCQGITLDEFRPRGSRRI
                     QRQAPEYDPEEIALLKLLRA"
BASE COUNT         1168 a         1804 c         2013 g         1038 t
ORIGIN      
        1 cgagcctctc ttcacagaga acccgccggc agaaggcgtg cgctgccgct cgtcaaacat
       61 aggaaagcaa accggatgcc tgcacaattt aagacgcgcg ctgcgcgcct gtcaagaaag
      121 ggctaacgcg accgccacta attgcgtgcg gcgcgcagag gttggcagcc gccgacagcc
      181 acgagcagag agacgcggtt gtgatgccct gcccctctcc ggtctgcgct ctctttggct
      241 gtcggcctac tgcctgcggc ggcgtcgaac cttcgcgacg accccggcca gccccgtgcc
      301 gagcaggagc aacgtcgccg gttccggcac gggttcgggg ggaggcgcga aggcgaaagt
      361 cgcctgcacg aggctgctgg tgccgttatt taccggcaag aagatgaacg tggcggttcc
      421 cgctcccgtc aggtcaaagg tgaatggcgc cgacccatcg ctggggtcta cggtgattct
      481 accgatgaaa ctgaagggcg agacaatcgg actgtaatcc gccgggacgg tgagcagcgg
      541 cagagtgatg aaattatcgc cgctgataac cacgtagccc acgccggggc ggtaggtggt
      601 gccgttgtag gtcaggttga ggatcgtgtc gatccgcacg gggaacgtgc cgccgaattg
      661 tctgccgggc tggcacaggc cgcaaagctg cgtctcaccg gatgacgtgt tggagccgtg
      721 aaaactgaag ttcagcccgg tcacgtttag attgaagttg ttgaggccaa tcggcgtggt
      781 ggccgtgccg cccgtgatga cgagcgggac ggcgcgcgcg gtcgaagcgc agcacaggag
      841 catgagcggc aggaaaagca ggaaggttag agttttgaaa gtgccaagag agacagttgg
      901 ggcgcgaagc ggcaagggac gcatggtggt gattctcctt aattcggtgg ttggctgggg
      961 ggacaaggta tacccgccgg ggcagtctgt aaagggagag aggccgcgaa gagagtttga
     1021 cgctacgcct gaaagggagt ttgacgctac tcgtcagcga cagtttgacg ctttacgcca
     1081 gcgacagttt gacgctttac gccagcgaca gttcaacgct tcgcctttac ccctccttct
     1141 tttaagatga ccgcatgagg attcaaccgg cgcgcagatt gagagggcgc gtgctcgtgc
     1201 ccggcgacaa atcgatctcg caccgcgccg ccatcatcag tgcgctcgcc gggacgggcg
     1261 ggcgttcgcg gctcacaaat tattcgacga gcgcggactg tgccgccacg cttgactgcc
     1321 tccgcgcgct cggcgtgcga atcgagcgcg acggcaacaa cgtccaagtc gaaggcaggg
     1381 gaacgcgctg gaccttggag gaagcagacg aaccccgcgc gccgctcgac tgcggcaaca
     1441 gcggcaccac catgcgcctc ttagccggac tactcgcggg acagcccttc acttcaactc
     1501 tcacgggcga tgcttcgctc cgctcgcgcc cgatgaagcg gatcatcgaa cccttagagt
     1561 tgatgggagc gcgaatttca tccgccgcag gacgcgcgcc gcttcgcatc gaagcacgcc
     1621 gctcaccgct cgcgcccgtg cgctacgcgc tgcccgtcgc cagcgcgcag gtcaagtcgt
     1681 gcctgctgct cgccgctttg aacgccggag ggcgaagcga gatcatcgaa gcgcgcggcg
     1741 caacgcgcga ccacacggag cgtctgctct cgctcttcgg cgccagcgtc cagacggagg
     1801 cgacaactta cgaaggccgc accgtgcccg tcgtctcgct gcaaactccc gtgagcctcg
     1861 aagcgcgcga ttgcgatatt gcgggcgaca tctcttcagc ggccttcttt atcgccgccg
     1921 ccgcgctgct ccccggttcc gagctgacgc ttcccgccgt cggcctcaac ccgacgcgca
     1981 cggaaattct ccacacgctg cgaaagctcg gcgcggaagt ctctgtcgcg ggcgagagca
     2041 cggagtcggg cgaagcggtc ggcgaactac gcgtgcgcgg cggactcgcg ccgacgactc
     2101 cgaccgcagc ggcggcgacc actacggcgg acgcacatgt cttgcgcggc gagttgatcg
     2161 cagggcttat tgacgagcta ccgatcctct gcgtggtcgg gacacagatc gaaggcgggt
     2221 tggagattcg agacgcctcg gagttgcgcg tcaaggagac ggatcgcatc agcgcgatgg
     2281 tcaaaaacct gcgtgcgctc ggagcgggcg tggaggagtt cgcggacggc ctgcgtatcg
     2341 aagggcgcac gcctctgcgc ggggcgcggc tggaatcttt cggcgatcac cgcatcgcga
     2401 tggcctgcgc cgtcgccgcg ctcatcgcgg aaggcgagtc agaactggcg ggcgcagatg
     2461 cggtcgccgt ctccttcccc gaatttttcg ggatgctcga atcggtgacg gagcgatgaa
     2521 ggcgggcgcg cgtcgcatcg ttctcacggg cttcatgtgc gcgggcaaaa cgacggtcgc
     2581 acgtgcgctc gccgcgcgcc tcgattgcct gatgctcgat acggatgccg tcgtcgtcga
     2641 acgcacaggc cgccccgtcg aagcgatctt tgcagaggac ggcgaagcgc gcttccgtca
     2701 aatcgaaagg aaagtgttgc gcgattgttt ggagaacgca gcggcgcgtg tcatcgcgct
     2761 cggcggcggg acgtggacgc tcgcagagaa tcgcgcgctc atcgccgcct acgattgcct
     2821 gaccatctgg ctcgacgcgc ccttcgcact ctgctggcaa cgcatcacag cagccgtggc
     2881 gagagacgaa accgcgcgcc cgctcgcacg caaccacgag catgcgctcg cgctctatac
     2941 gtcgcggcgt gatgcttaca gcctcgcggc gcggcgcgtg caggcaggcg aagagcacag
     3001 cgcggaagac accgcgacgg agatcatgtc tataatcgag cgcgagaatt tttagcagca
     3061 gcaacgaaca cggaagggac gagaggaaga gaacccgaat ggctaacaga gacgcagacg
     3121 ccaaagcatt gaaggtcgcc aagaaactct tgaacgcgga gaactggaaa cacctcgcgc
     3181 gctggcacgg cgacggctac tacgaactcc acacggaaga cacgggcgac acacccgtcc
     3241 gcctctttct tacaaagcaa cttttagacg acgccgagga catactctat cgccagatcg
     3301 tcaacgcgac gcgctttccc ggcgtccgca tggtcgtcat cacgcccgac acgcactatg
     3361 gctacggcgt ccccgtcggc tgcgtcctca tcacagacgc cgacgcaggc gcagtcgcaa
     3421 tgggtccggt cggctacgac atcggctgcg ggatgatgtc ggcgcgttcg gaggtggagg
     3481 cggagcgtgc gacgccggag aagcgtctgg agttcaatcg cgcggtgatg gagcgcgtgg
     3541 atatgggcgc gggcgggaaa agcgtcaggt tgggtgagtt gtcgaagagt gagtttaata
     3601 atctggtgcg cgggggcgcg gagtattacg tcgaaaaata tggcgcgaca tttgatcgga
     3661 gtcgcgcgga gcggcaccgg attccggtgg acgacgattg gcagattccc tggggcggga
     3721 agggcaggcc ggagcgcggc ctgaaccaga tgggcagcct cggcggcggc aaccatttca
     3781 tcgaactgca acgctcggag cagacgggca cggtcttcgt gcaggtgcac acgggcagtc
     3841 gcggcttcgg gcatggtctg gcgaccaact atttcgagat ggcgcgtgag gaacgtcccg
     3901 aagcgatcac ggacatagac cttggctact tcacgcccga ctcacgccac tacagcagct
     3961 atctgaacgc cgtcgcggcg ggcggcaact acgcgatcct gaatcgcctg atcatcttcg
     4021 agcaggtgtc ggacgctttc cgcgaagtgt tcggtcagga gcttgaactg atttatgaga
     4081 tcagccacaa cctcgtgcag cgcgagacgc acccggagtt cggcgaggtg tgggtgcatc
     4141 gcaagggcgc gacgcgtgcg tttcccgcag ggcacccggc gctcgcgggc acgcactggg
     4201 aagcgaccgg ccatcccgtc ttaatccccg gctcgaacaa agactggagc tacatcctgc
     4261 aaccgctggc gggcgcggtc aacagcggct actcggtgaa tcacggcgcg gggcgcaggc
     4321 tctcgcgcgg cgaggcttcg cgcacgctct cgcagcgcgc cattgacgac gagtacaagg
     4381 ccgccggaat tctggtcaac atcaacgggc gcgtgccgct cgacgaagcg gctcccgctt
     4441 acaagtcttc gcaggccgtg atcgaggcgg tggtcggcgc ggggctggcg cgcatcgagc
     4501 atcaactctg gccgctcgcc tcgctcaaag gcgcggacga acgcaaaggc aaacgtcgcg
     4561 gcaaaggcgg caaggcagac acaggcgacg cgaagcaggg acgcaagacg aaagagcact
     4621 actgacgcaa ttttcactcg actgtcaggc aagcaataaa actgaacgcc gccgcaggcg
     4681 cagcgcaaag ccgcaaagtg agcgcagaga aatttaactc tctgcgctca ctctgcgttc
     4741 cgctctgcgt ctgcggcggc gttgaatctt tcctgcctga cactcaggcg agaagcgttc
     4801 taaactttcc gttaagctac gcgcgcagca gtttcaagag ggcgatctct tcggggtcat
     4861 attcgggagc ttgacgttga atgcggcggc tgccgcgcgg tcgaaattcg tcgagcgtga
     4921 tgccttgaca gtagcgttcg ataactgccg ggtggatgta gctggaacgg cagacggtcg
     4981 gggtgttgcc gagatgttcg gccaccgagc ggatggcgtg gaggatgttg cgcttcgcct
     5041 gcttgtcgtc ggcgggcttg ccgagttcgg cgagcgagac ggcgcacagg agcgtgccgc
     5101 cccaagtgcg aaagtctttg gcggaaaatt cgggcgcggt ggcggctttg atgtattcgt
     5161 tcacgtcgcg cggcttgatg gcgacggggc ggcggttcgc ttcgtcccag tattgaaaga
     5221 gacgtttgcc gcgcagggat tgaatgtctc tgaggagcgt cgccaactct tcatcgacga
     5281 ggatgcgccg gtgtttgatg tggtgcttgc cgatgaagct gaagatgagg cgtccgccgc
     5341 gtttgaattc cagatgacga ttgcgcagcg tcgtgatgcc gtaggtgcgg taacgcttga
     5401 cgctctcttc ggagccgacg cggaaataga gatcgttgat gaggcggacg atgacggcga
     5461 gcacgcgttc gcgcggcaac ccttcgcgcg cgatgtcttc gttggtgcgg cgacgcaggt
     5521 gtttgagttg ttcgccgaag cgttcgatct tcgcgtactt gcgtcgcgcc tgcctcgcag
     5581 cgaaggccgg gtgatagatg cgctgcacgc gcccgcccgc gtcgaggccg atggcttgca
     5641 ggcggtggcg cgacgaaggc gagatgcgca cctccttcca cgccgggggg atgacgagcg
     5701 acttgatgcg ttcgagcgcg gcctcgtccg tgatgcgtcg agccgccgcg tcttcgtagc
     5761 ggaagccgct gcgcttcgtg ccgcgccgtc gccaccagcg cgcacgcgcc ccgcgcgcca
     5821 cgctctccgc cgcgctcttg atcgcaagtt tatcttccaa cggcgcttgt acactcaaca
     5881 tcactgaaac cgtctcctgc ctgacagagt ttaagcgaga catgttaact gccgggcggt
     5941 tacgaaacta tcgcggcgcg gaaaacagag agtctgcgac gggtaaatta aacgcgaaga
     6001 gacaaagaca cgaagaaaac ttt
//
LOCUS       DASWNS010000382         6072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_65877, whole genome shotgun sequence.
ACCESSION   DASWNS010000382 DASWNS010000000
VERSION     DASWNS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65877"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1380)
                     /locus_tag="VGB76_13460"
     CDS             complement(124..1380)
                     /locus_tag="VGB76_13460"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEY0099950.1"
                     /translation="MPPEHKNGSAKKLKRTAGNEPLEATAPQATKHKAATPQATARKA
                     SAQKTTTRKKAATHTSQQSHASQKSQRSRARSVSISSPASSQSAPTLASASSRRPASP
                     DAPASPTRPSVAIVGAGRLGSALALALSQSGYSIVALVARRPQHARRAARLLSPRPLA
                     LGSTQLDSLPETDLLLITTPDDEIAAVASRLAQTFAAREAKATPPLRRRPARVALHAS
                     GALVSDALAPLGAHAFSLGSLHPLVSISDPLGGASNLRGAFYCLEGEARARAAADKIV
                     RALGGRSFSIAARRKPLYHAAAVMCSGHLVALFSLATEMLVRCGLPRHQASSVLLPLA
                     SGTLENLARRAHPASALTGPFPRADVATLRRHVAALRELEMNETLAVYVALGRHALRL
                     ARSSGGGDAASRAALAEIEALLAGAP"
     gene            complement(1370..5098)
                     /locus_tag="VGB76_13465"
     CDS             complement(1370..5098)
                     /locus_tag="VGB76_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099951.1"
                     /translation="MKRTQDKIKDLVEPQEFDDLRDFAADPARALAAYRFTDATSDLL
                     ARWLDALADLPRGHGAAHALAGMRGVGKSHTLATFGALIAASDEHHHAQITDAHVAAS
                     ARRLAARRHLLVRVARGTRPTLRDELVDAFIATCGGSAADWTGDPAAWIAQAAARTRT
                     TATIVIIADTAFGREARVKRDDGPLLSELAVAASGANAFVALALDDDIAGAEGVNVAL
                     ARTFQIDYLEPEHLYHVANIYVLRKNAPARDALHEIYLSLRQSVPNFNWSEPRFAALY
                     PVHPLVADTAASVRLYAPAFAFLPFAAQAARNAINRPALSLILLDEVFDHSERDLRRS
                     DDLKDAFRVYDELATKAVSQFPAMQRLQVRLVLKSLFILSLDGRGATARDLCAALLFQ
                     DENSLQAAIERVGETLERLSELAPKGGLSAQSDGGELRYRFQVNAAADFDAALAAASE
                     SPSIDAAAINSLLRTLPRGRFEDWPFSDGEPESLKGEPESADFEITWRGASRSGRLTR
                     QTSAPHAPAPDATNLITSDAASEVNSLASNHLGSHLGSHLRGSAGSVEYDWELLILEA
                     CAGEAGENVLAHAAHAKGAAPVSVVWQPAACSAEELSILRRLVALRRVDSTLGEFGET
                     ALAAANSLAAQAERIWSRLYIDAGVLHVGDERITFTDEAHAAQTLASMLADALAPRFT
                     AHFPQHPHFDASPTELQTARLVDNLFGGSNVADTDVQQLAETFAVPLGLATRRGDILI
                     PETGDTVLTQPWVREVLTLVDRAGSSVVPLGEVHRVLHGQPYGLLRQTQYLVLAALVA
                     QRRIELSTGGDDRITRRTLGSSIKWDEVAGVCRAAEIHHNAEELTNWARLLTGDATLP
                     SIADAGARESVRAALSAWLAAWRAETVLREFDALPDAGLTTRAWDLAAAARRTFGAAA
                     DAVADALAGNLSLEEGLQRVADIFGDAPEQLAQRATQLAALRNYTAGVVRRESARAYL
                     SAAELTSLEVIESARRELLLIADDPHTLFDPARTERFELLWQEFQAHYIEHYADVHAR
                     AVGQARDHARLDAFTRSAEWRDFEALAVLPFVAPQLWEEAASFVAEVRNARCDLPVAA
                     VLARHPRCACAFRLSRSAQFLEAATLLTEMTERGRAAYRRTLALFHAHLTHALNQLAS
                     DAPDADTASRAYALAHLFVQRRTPELLTQTDAHLIARALEQAPSALPLRVTLPEGVRR
                     GLVTREELAARWQQWFDELPHDAALVEIMVGNNNSDAA"
     gene            5585..>6072
                     /locus_tag="VGB76_13470"
     CDS             5585..>6072
                     /locus_tag="VGB76_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099952.1"
                     /translation="MRFWRFSAMFNQLSRTITALALVWGLLVGSTAALAAPQQQPKQA
                     SKSEQAAAEKKAREAAEKQDRDDAIEAAKQKQELAKNIGKPLSTDEDPSQIGKRKING
                     GFIASMSGSIEKEVSFGRENPLHHQSHRLGRSQRLRAARRLLLRQQGAALGRRQRGGD
                     RGR"
BASE COUNT         1076 a         1871 c         2134 g          991 t
ORIGIN      
        1 cagagttgaa gcgtgtcagg gaatttatag ttactaactc taacgccaaa agagcgcgac
       61 gcgcaagcaa actctctttg cagcgcgtcg cgctcttcgg attttcgcaa ttcgcgcgcg
      121 cagttaaggc gcgcctgcga gcaaggcttc tatctcggcc agggctgcgc ggctggccgc
      181 atcgccgccg ccgctgctac gcgcgaggcg gagcgcatgt cgcccgagcg cgacgtaaac
      241 agcgagcgtc tcattcatct ccaactcacg aagcgcggcg acgtgacgac gcagggtggc
      301 gacatcggcg cgcgggaatg gtccggtgag cgcgctcgcg ggatgcgcgc gacgtgcgag
      361 attttcgagc gtgccggagg cgagcggcaa gagcacgctc gacgcctgat gccgggggag
      421 gccgcagcgc acgagcatct ccgtcgccag cgagaagagc gcgacgaggt ggccggaaca
      481 catcacggca gccgcgtgat aaagcggctt ccgccgcgcg gcgatagaga aactccggcc
      541 accgagcgcg cggacgatct tatccgccgc cgcacgcgcg cgcgcctcac cttcgagaca
      601 atagaaagcc ccgcgcaagt tcgacgcgcc gccgagcggg tcgctgattg aaacgagcgg
      661 gtggagcgag ccgagactga aagcgtgtgc gccgagcggt gcgagcgcgt ccgagacgag
      721 cgcgccgctc gcgtgcagag cgacgcgcgc ggggcgacga cgtaacggcg gcgtcgcttt
      781 cgcctctctc gccgcgaacg tttgtgcgag acgcgaggcg actgcggcga tctcgtcgtc
      841 gggggtcgtg atgagtagaa ggtctgtctc gggaagcgag tcgagttgcg tcgagccgag
      901 cgcgagcggt cgtggggaga gcaagcgcgc ggcgcggcgt gcgtgttgcg ggcggcgcgc
      961 gacgagcgcg actatcgaat aaccgctctg actgagcgcg agcgcgagcg cagagccgag
     1021 gcgtcccgcg ccgacaatgg cgacgctcgg acgcgtcggc gaagcgggtg cgtccgggga
     1081 agcaggccgt cttgacgaag cggatgcaag cgtcggagcg gattgtgacg aagcggggct
     1141 tgagattgag acggaacgag cgcgcgaccg ctgcgacttc tgcgaagcgt gcgactgctg
     1201 cgaagtgtgc gtcgcggcct tcttgcgtgt ggtcgttttc tgcgccgacg ctttgcgcgc
     1261 ggtcgcttgg ggcgtcgctg ctttgtgctt cgtcgcttgc ggcgctgtgg cttcaagcgg
     1321 ttcgttgccg gcagttctct ttaatttttt cgcgcttcca ttcttgtgct caggcggcat
     1381 cgctgttgtt gttcccaacc atgatctcga cgagcgccgc gtcatgcggg agttcatcaa
     1441 accactgttg ccagcgcgcc gcaagttctt cgcgggtgac aaggccgcgc cgcacgcctt
     1501 ccggcagcgt tacgcgcaac ggcagcgcag acggagcctg ctctaaggcg cgtgcgatga
     1561 ggtgtgcgtc ggtctgcgtc aaaagctccg gagtgcgacg ttggacgaag aggtgcgcga
     1621 gagcataggc gcggctggcc gtgtccgcgt cgggcgcgtc gctcgcgagt tggttgaggg
     1681 cgtgtgtgag atgcgcgtga aagagcgcga gcgtgcggcg ataggcggcg cgaccgcgct
     1741 ccgtcatctc ggtcaggagc gtggcggctt ccagaaactg cgcggagcgc gagaggcgaa
     1801 acgcacaggc gcagcgcggg tggcgcgcga gcacggcggc gaccggaaga tcgcaacgcg
     1861 cgtttcgcac ctcggcgacg aacgacgcgg cctcttccca tagttgcggc gcgacgaagg
     1921 gcagcacggc gagggcttcg aagtcgcgcc actccgcgct gcgtgtaaac gcgtcgaggc
     1981 gggcatggtc gcgcgcctgc ccgacggcgc gcgcgtgcac gtctgcgtag tgctctatgt
     2041 agtgcgcttg aaattcctgc cagagcagtt cgaagcgttc ggtgcgcgcc gggtcgaaga
     2101 gcgtgtgcgg gtcgtctgcg atgagcagca gttcgcggcg tgcgctttcg atcacttcga
     2161 gcgaggtcaa ttcggcggcg gacaaataag cgcgcgcgct ttcgcgtcgc acgacacctg
     2221 ccgtgtagtt gcgcagggct gcgagttgcg tggcgcgctg cgcgagttgt tcgggcgcgt
     2281 cgccgaagat gtccgcgacg cgctgcaagc cctcttcgag cgagaggttt ccggcgagcg
     2341 cgtcggccac ggcatcggcg gctgcgccga acgtgcggcg ggcggcggcg gccaaatccc
     2401 acgcgcgcgt cgtgagtccg gcgtcgggca gcgcgtcgaa ctcacgcaag accgtctccg
     2461 cgcgccaggc cgcgagccag gcggagagcg cggcgcgtac gctctcacgc gcgcccgcgt
     2521 cggcgattga agggagcgtc gcatcgcccg tcaggaggcg cgcccagttc gtcaactcct
     2581 cggcgttgtg atgaatctca gcggcgcggc aaactccggc cacctcgtcc cacttgatgg
     2641 aagagccgag cgtgcggcgc gtgatgcggt cgtcgccccc ggtcgagagt tctatgcgac
     2701 gctgcgcgac gagcgcggcg agcacgagat actgagtctg ccgcaacaac ccgtacggct
     2761 ggccgtgcag gacgcgatgc acttcgccga gcggcaccac gctgcttccg gcgcggtcaa
     2821 cgagcgtgag cacttcgcgc acccaaggct gcgtgagcac ggtgtcgccc gtctccggga
     2881 tcaggatgtc gccgcgccgc gtggcgagtc cgagcgggac ggcgaaagtt tcggcgagtt
     2941 gttgcacgtc cgtatcggcg acattactgc cgccgaacag gttgtcaacc agacgcgccg
     3001 tttgcagttc ggtgggcgac gcgtcgaagt gcgggtgttg gggaaagtgt gcggtgaagc
     3061 gcggggcgag cgcgtcggcg agcatcgagg cgagggtttg cgcggcgtgc gcttcgtctg
     3121 tgaaggtgat gcgctcgtcg ccgacgtgga gcacgcccgc gtcaatgtag aggcgcgacc
     3181 agatgcgctc ggcctgcgcg gcgagcgagt tggcggcggc aagcgcagtc tcgccgaact
     3241 ccccgagcgt cgaatcaacc cgccgcagcg caaccagacg ccgcaggata ctcaactctt
     3301 ccgctgaaca ggccgccggt tgccagacca ccgagacggg cgcggctccc tttgcatgcg
     3361 cggcatgggc gaggacattt tcgccagcct ctcccgcgca cgcttcaagg atcaacaatt
     3421 cccaatcgta ctctacgctt ccggcactgc ccctgagatg gctgccgaga tggctgccga
     3481 gatgattgct ggcaaggctg ttgacctcgc tcgcggcatc gctcgtaata aggtttgtcg
     3541 catctggcgc gggcgcatgc ggggcggaag tctgccgcgt gagacgaccg gagcgcgacg
     3601 cgccgcgcca cgtgatctca aagtctgcgc tctccggctc tcccttgagc gattcaggct
     3661 cgccgtcgct gaaaggccaa tcttcaaaac gcccgcgcgg aagcgtgcgc agcagagagt
     3721 tgatcgccgc cgcatcaatc gaaggcgact cgcttgcggc tgccagcgcg gcatcgaagt
     3781 cagccgccgc gttgacctga aaacgataac gcagttcacc gccgtcggac tgcgcggaga
     3841 gtccgccctt cggggcgagt tcgctcagac gctctaaagt ctccccgacg cgttcgatgg
     3901 ccgcttgcaa agagttctcg tcttgaaaca ggagcgcggc gcagaggtcg cgcgccgtcg
     3961 cgccgcgccc gtcgagcgag aggatgaaga gacttttcag cacgagcctg acttgcagac
     4021 gctgcatcgc cggaaactgc gagacggctt tcgtcgccag ttcgtcatag acccggaaag
     4081 cgtctttcag atcgtccgaa cggcgcaaat cgcgctcgct gtgatcgaac acttcgtcga
     4141 gcaggatgag cgagagcgcg gggcgattga tggcgttgcg cgcggcctgc gcggcaaagg
     4201 gcaggaaggc gaaggccggg gcatagaggc gcacggaggc cgccgtgtcc gcgacgagcg
     4261 ggtggacggg atagagcgcg gcaaagcgcg gctcgctcca gttgaagttc ggcacggact
     4321 ggcgcagcga gagataaatc tcgtgcagcg catcgcgcgc cggggcgttc ttgcgcagca
     4381 cgtagatgtt ggcgacgtga tagaggtgtt cgggttcgag ataatcgatc tggaaggtgc
     4441 gcgcgagcgc gacgttgacg ccttccgcgc ccgcgatgtc gtcgtcgagc gcgagcgcga
     4501 caaaagcgtt tgcgccgctg gcggcgacgg cgagttcgct gagcagcggc ccgtcgtcgc
     4561 gcttgacgcg agcctcgcgc ccgaaggccg tatcggcgat gatgacaatg gtggccgtcg
     4621 tgcgcgtgcg cgcggcggcc tgtgcgatcc aggcggcagg atcgcccgtc caatccgccg
     4681 cgctcccgcc gcacgtcgcg atgaacgcat cgacgagttc atccctgagc gtcgggcgcg
     4741 tgccgcgtgc gacgcggacg aggaggtggc ggcgcgcggc cagacgcctt gcgctcgcgg
     4801 cgacgtgcgc gtccgtgatc tgcgcgtggt ggtgttcgtc cgaggcggcg atcagcgcgc
     4861 cgaaggtggc gagcgtgtgg cttttgccga cgccgcgcat ccctgcgagc gcgtgcgccg
     4921 cgccgtgccc gcgtggcagg tctgcgagcg cgtcgagcca gcgtgcgagc aggtcggaag
     4981 tggcgtcggt aaagcgatag gcggcgagcg cgcgtgcagg gtcggcggca aaatcccgca
     5041 ggtcgtcgaa ctcctgcggt tcaaccaaat ccttgatctt atcctgtgtt cgtttcattc
     5101 gctggtggag tcgggaaaag tcggggcaaa tagcgaagag aggggaaata gaccgcgacg
     5161 ccgaacgcat cacggagggc gctttcgact gtaacacgcg cgcgcgaacg ggcgcaatca
     5221 ctatactttt ctcgcccgcc gcgtgacaca aactctacac ttttgcccgt gccttgctat
     5281 aatggcgcga gcgaagcaac caacgtccgg cgggcagagg cgaaaggttt tacaggcagc
     5341 gaaccgtggc gcgtgcacgc ccgcgtcgga cggcgaacca cttgagcttc tccgtgcatc
     5401 aaagaatcac gcgctttcga acgccagaac cggcattgtc cacagctttg caggcgcggc
     5461 gaccgtcgca gcagagcgcg ttcctgacgc atagataaat tttcacgtcg caccaagtta
     5521 tatttgcgac ttcggccaag agacaacggg atggacaaca ctccccggcg gccatagttg
     5581 ttagatgaga ttctggagat tttccgccat gttcaaccaa ctttcacgca ccatcaccgc
     5641 gctcgcgctc gtctggggtc tgctcgtagg ctcgacggct gcgctggccg cgccgcagca
     5701 gcagcccaaa caagcctcga agagcgagca ggccgctgcc gaaaagaagg ccagagaagc
     5761 cgctgaaaag caggacagag acgacgccat cgaggcggcc aagcagaaac aggaacttgc
     5821 caagaacatc ggcaagccgc tctccaccga cgaagacccg agccagatcg gcaaacgcaa
     5881 gatcaacggc ggctttatcg cttcgatgag cggttcaatc gagaaagaag tctccttcgg
     5941 acgcgaaaat ccccttcacc atcaaagtca tcgactcgga cgaagtcaac gccttcgcgc
     6001 tgcccggcgg cttcttctac gtcaacaagg ggctgctctt ggccgccgac aacgaggcgg
     6061 agatcgcggg cg
//
LOCUS       DASWNS010000383         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_93409, whole genome shotgun sequence.
ACCESSION   DASWNS010000383 DASWNS010000000
VERSION     DASWNS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93409"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /gene="menC"
                     /locus_tag="VGB76_13475"
     CDS             <1..993
                     /gene="menC"
                     /locus_tag="VGB76_13475"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEY0099953.1"
                     /translation="VEGWGECTAGEGPFYSEEWTESAWSVIKTYLAPLTLGREMERAA
                     DASALMRAVRGNRMAKAAIETAVWEIEARRANVPLWKLLGGVRREIACGVSIGIQDSP
                     EALMEKIEREIGAGYQRIKIKIKPGWDVSIVERVRARFPDIGLMVDANSAYTLADAPL
                     FRELDKYNLMMVEQPLAYDDMFDHARLQEQIATPVCLDESIRSSEDARKASELGACRI
                     VNVKLGRVGGHFEAQEVERVCRERNIPVWCGGMLESGIGRAHNIAMATLAGFTLPGDV
                     SASARYWEEEIIEPPVTVSARGTIDAPDAPGIGFEIKRERIDALTVRRESYTPA"
     gene            1034..2206
                     /locus_tag="VGB76_13480"
     CDS             1034..2206
                     /locus_tag="VGB76_13480"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY0099954.1"
                     /translation="MPVELDKRDAAALHEYFSGRESEMLSFMRALVEAESPSGDLDGS
                     HAVVSLLAGAAREIGLETEVTFEYDPAYGTHLLIRAFGEHKTDAPATLILGHTDTVHS
                     RGSTQARPWREEDGRIYAPGIFDMKANCALAVESLRACATLKLQPTHPVVILLTCDEE
                     VGSQSGRALVEAEARRAAQVLVFEPSAPGGRAKTARKGTSSYTVRAAGIAAHAGLDPE
                     KGASAILEIARQIERLHALGDAARGVTVNAGVVSGGTRSNVVAAEAETEVDVRFSTMD
                     DALRMEETILNLRPFDERVKLSVEGAINRPPLERSEAVVALYEHARGIASALDFELGE
                     ASVGGASDGNFAAAVGAVVLDGLGIAGDGAHAAHEHINKSDIARRGAFLSGLLVTL"
     gene            2226..2447
                     /locus_tag="VGB76_13485"
     CDS             2226..2447
                     /locus_tag="VGB76_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0099955.1"
                     /translation="MRPSFRGRQLGRRLTVAIIERARALGYERLRLDTLPTMHEAIAL
                     YTSLGFERIEPYRYNPIAGTLYMELYLRR"
     gene            2477..>3298
                     /locus_tag="VGB76_13490"
     CDS             2477..>3298
                     /locus_tag="VGB76_13490"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0099956.1"
                     /translation="MSALRRITLFFLLALLFINAPLAPSRLEAARPNAAARAASRDPV
                     RAPRGMVASTNKLASEVGVDILKRGGNAVDAAIAVAFALAVTYPAAGNLGGGGFMMIR
                     LRDGRTTAIDYREMAPLAATRNVYLDAQGNLIKGEGSSTVGYRAAGVPGTVAGMALAL
                     RKYGSGRLSWAQLAEPARRLAAEGFTISHQFARGLRASDDLLGRYADSRRIFLNNGRY
                     FKEGDTLRQPELAATFARLQRGGAREFYEGETARLIAADMQRNKGLMTLADLRGYE"
BASE COUNT          618 a          962 c         1139 g          579 t
ORIGIN      
        1 gtggaaggct ggggcgaatg taccgcaggc gaaggacctt tctatagcga agagtggacg
       61 gagagcgctt ggagcgtgat caaaacttat cttgcgccgc tgacgctcgg gcgcgagatg
      121 gagcgtgcgg cggatgcctc cgcgttaatg cgcgcggtgc gtggaaaccg catggcgaag
      181 gccgcgattg agacggctgt gtgggagatc gaagcgcggc gcgcaaacgt gccgctctgg
      241 aaactgctgg gcggcgtgcg cagggagatc gcgtgcggcg tctcaatcgg cattcaggat
      301 tcgcccgaag cgttaatgga aaagatcgaa cgcgaaatcg gcgcgggcta ccagcgcatc
      361 aagatcaaga tcaagcccgg ttgggatgtc tcgattgtcg agcgcgtgcg ggcgcgcttc
      421 ccggacattg gattgatggt ggacgctaac tcggcttaca cgctcgcaga cgctccgctc
      481 tttcgtgagt tggacaaata taacctgatg atggtggagc agccgctcgc ctacgacgac
      541 atgttcgacc acgcgcgctt gcaggagcag attgcgacgc ccgtgtgtct cgacgagtct
      601 atccgttcga gcgaggatgc gcgcaaggcg tcggagttgg gtgcgtgtcg catcgtgaac
      661 gtgaaactgg gtcgggtcgg cgggcacttc gaggcgcagg aagtggagcg cgtgtgtcgc
      721 gaacgaaaca ttcccgtctg gtgcggcggc atgttggagt cgggcatcgg gcgggcgcac
      781 aacatcgcga tggcgacgct cgccgggttc acattgcccg gcgatgtctc tgcgagcgcg
      841 cgttactggg aagaagagat catcgagccg cccgttaccg tctcggcgcg cggaacgatt
      901 gacgcgccgg atgcgccggg catcgggttt gaaattaaga gagaaagaat tgacgcgctg
      961 actgtgaggc gtgagagcta cacgcccgct tgatttcagg tatcagccgg gaagtttaat
     1021 aataggaatc aacatgcctg tcgaattaga caagcgcgac gccgccgcgc tgcacgaata
     1081 tttttcaggg cgcgaaagcg aaatgttgtc gtttatgcgc gcgctggtcg aagcggaatc
     1141 tccgtccggc gatctggatg gcagccacgc ggtcgtctcc ctgctcgcgg gagcggcgcg
     1201 cgagatcggt ctggaaacgg aagtcacgtt tgagtatgac ccggcctacg gcacgcacct
     1261 gctgattcgc gccttcggcg agcacaagac agatgccccg gcgacgctca tcctcggaca
     1321 cacggacaca gtacattcac gcggctcgac gcaggcgcga ccgtggcgcg aagaagacgg
     1381 gcgcatctat gctcccggca tcttcgacat gaaggccaac tgcgcgctcg ccgtcgaatc
     1441 tctgcgcgcg tgcgcgacgc tcaaactgca accgactcac ccggtcgtca tcctcctgac
     1501 gtgcgacgaa gaggtgggca gccagagcgg gcgcgccctc gtggaagcgg aggcgcggcg
     1561 cgcggcgcag gtgctcgtct tcgaaccctc cgcgccgggc gggcgcgcga agaccgcacg
     1621 caagggaacg agcagctaca ctgtgcgcgc cgcaggcatc gccgcacacg cgggactcga
     1681 cccggagaag ggagcgagcg cgattctgga gatcgcacga cagatcgagc ggcttcatgc
     1741 gttgggcgac gcagcgcgcg gcgtgaccgt caacgcgggc gtcgtgtccg gcggtacgcg
     1801 ttcaaatgtg gttgccgccg aagcggagac ggaggttgac gtgcgtttca gcacgatgga
     1861 cgatgcgctg cggatggaag agacgatctt gaatctgcgc ccgttcgacg agcgcgtcaa
     1921 actctccgtg gagggcgcga tcaatcgccc gccgctcgaa agaagcgagg ccgtcgtcgc
     1981 gctctacgag cacgcgcggg gcatcgcttc ggcattggac ttcgagttgg gcgaggcgag
     2041 cgtgggcggg gcatcggatg gaaacttcgc ggcggcggtc ggcgcggttg tgctcgacgg
     2101 gctgggcatc gcaggcgacg gcgcacacgc cgcgcacgag cacatcaata agagcgacat
     2161 cgcgcgtcgc ggtgcatttt tatccgggct gctggtcacg ctgtaaattg gaagcgtcgc
     2221 agtacgtgag gccgtcgttt cgcggacgac aactcgggag acggttgacc gtcgccatta
     2281 tcgaacgggc acgcgcgctc ggctacgaac gcctgaggct cgacacgctc ccgacaatgc
     2341 acgaagcgat tgcgctttac acctcgctcg gattcgagcg catcgagcct tatcgctaca
     2401 acccgattgc gggcacgctc tacatggaat tatatttgcg ccgatgattg aaaaaaaatt
     2461 gagaggaaca agattcatgt ccgcactgcg ccgcatcacc ctcttttttc tgctcgcgct
     2521 tctcttcatc aacgcgccgc tcgctccttc gcgcctcgaa gcggcgcgcc cgaacgccgc
     2581 cgcgcgcgcc gcttcgcgcg accctgtacg cgcgccgcgc gggatggtcg cctccacgaa
     2641 taagctcgcc tccgaagttg gcgtggatat tttgaagcgc ggcggaaacg cggttgatgc
     2701 ggcgatagcc gtagccttcg cgttggcggt tacgtaccct gcggcgggca atctcggcgg
     2761 cggcggcttc atgatgattc gcctccgcga cgggcggacg actgcgattg attaccgcga
     2821 gatggctccg ctggcggcga cgcgcaacgt ctatctggac gcgcagggaa atctcatcaa
     2881 aggcgaaggc tcctcaaccg tcgggtatcg cgcggcgggc gttcccggca cggtggcggg
     2941 gatggctctg gctttgcgga agtacggctc agggcggctg tcgtgggcgc agttggcgga
     3001 gcctgcgcgg cggctcgcgg cggagggctt caccatcagt catcaattcg cgcgaggttt
     3061 gcgcgcgagc gacgacctgc tcggtcgtta cgcggacagc agacgcatct ttctgaacaa
     3121 cggtcgttac ttcaaagagg gcgacacgct gcgccaaccg gaactcgcgg cgaccttcgc
     3181 gcgcttgcag cgtggcggcg cgcgggagtt ttacgagggc gagacggcgc ggctgatcgc
     3241 agcggacatg cagcgcaaca agggcttgat gacgctcgca gatttgcgcg gctacgag
//
LOCUS       DASWNS010000384         6807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_300038, whole genome shotgun sequence.
ACCESSION   DASWNS010000384 DASWNS010000000
VERSION     DASWNS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300038"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3093
                     /locus_tag="VGB76_13495"
     CDS             <1..3093
                     /locus_tag="VGB76_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099957.1"
                     /translation="GVMFQFDMKINKAGWHFPQSRIESLWEDWKPTITGMRAPEPLFF
                     RANSNDCVTFHHANLIPKVYVQDDFQVRTPTDVVGQHIHLVKFDVMASDGAGNGWNYE
                     DGTFGPQEVEHRIKHINEVGGIFVSKDSIYSSRQRLLEIQKHPSFDAATQVTFQRWFV
                     DDVRDRDGNDRTLRTVFTHDHFGPSTHQQTGLYAGLVVEPKGSIWRQNESPGALLGGA
                     GPIRLRDDGGPTSFAAVIIPPDQTQSYREFLLEYQDFHLAYEAGGGGTAENPVPDPAR
                     AINPPGRNEIPLPRMIMGGPPFGPETALLKPPVDGKCPDGEPAPCPEIVSADDPGTFT
                     VNYRNEPIALRVRDPRTNTQAPGDFGDLSLAYASIKRLDRRLNTAGPYPPLTPGMSIY
                     DPFTPLLRANENDRVQVRILVGAHEEGHNFTIHGVKWLFEPSDPDSGWRNSQMTGISE
                     KFDFILAGRPPEGPPGSEFRADYLYKPGAAVDDQWNGLWGIMRVYKAGAAARTNTETD
                     SPEMPQGDVPPFELPSNPAFRTSTDSTSQPDQATIAEEEAEFVGPCPRRARPLHYNVA
                     AYLARDILPERRLEFNGQNGYTFTVTNNTNDPDDPSAGDTETGPLNDPTAILFVDQDN
                     DIDPETKMYKRKRVEPLLLRANAGDCITVTLTNNLPLEPVDLPGYNTMPMIVDYFNAN
                     QVVPSRRVGLHPQLVHYDVTTSDGMNVGLNPIQTVSPGESRTYTWYAGDVGVELSTNR
                     WYGKPVEFGATNLVSSDPIKHSNKSAIGSLIIEPYGSSWAVDEDSTHSAMIYRPVDIW
                     NAPCAGCPTQVDGFREFVLQFQNDLNMRFDSGRPVPNLAEGEDPEDSAQKAINYRTEP
                     FWTRLCYWPALPLTGNDQPIPARQCPASDAKQTRALDMTNSLHNQQIGGLDPVTPIFT
                     ADAGQPVRFRVLHSGGHARNNVFMVHGHLWQEMPYQDGSNSSVIGFKQDSPWHGSQWG
                     HGPSNHFDVVLESAGGAFGVQGDYLYRTFQSWQFDGGIWGLFRVERRVYDQPVDEIYY
                     "
     gene            complement(3157..3348)
                     /locus_tag="VGB76_13500"
     CDS             complement(3157..3348)
                     /locus_tag="VGB76_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099958.1"
                     /translation="MRSGVSNGAQDEAEAGDGWFISFHLSFIELFICVAGTVVADGDF
                     FSRRLIGCGACARRETGAA"
     gene            3645..5729
                     /locus_tag="VGB76_13505"
     CDS             3645..5729
                     /locus_tag="VGB76_13505"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY0099959.1"
                     /translation="MLTNTHWIRRTLGTKHTFGTKRTFGIRRALASALLLAAFASAHA
                     AQPQPQKPSPQTAQAPQPGVQTKASAPATVVASNAPQKITHEGVEIEFSIEPSTKSEG
                     LVEGKDANVRFTIRDTTTKQPLTNLRPAAWIDARKAGTTATDPKACREKVQAFLQSQL
                     TQRPDIDLNSYIILALNEEANISVIDPLLGFGTTKLRTLVFLKSPGEDWVMSADRKRL
                     YVSMPLVNQVAVVDTGSWKVLSNIDTGARPVRVRLQGDGKYLWVANDGLGDAPGGVTV
                     IDTATGKVAAAQIDTGAGHHEIAFSADDRFAFVSNRLDGTLSVIDVARLAKIKDLKTG
                     AQPASLAFSPAGRAIYVAHEGDGSIVSVDAVRHEILARVQAKAGLVALRFAPDGRHGF
                     VVNQKENAVHIFDASTNRLLHTVTVGKSPDQVAFTKQFAYVRSLGHEQVSMINLSHLQ
                     KGSISESSVARFSGGQKPPAQATSRSTAEAVVPSAEDGSVLVANPADRMIYYYQEGMA
                     APMGSFTNYKREPRSLLIWDASLRETAPGVYSTKAQLTGQGNYDVAFLLDSPRVVNCF
                     EMSVKENPEFEKKRGLAISIEPLLKDRNLRTGENVQLRFKVIDTTTGQPKTDLKDMGV
                     LTFLSPGIWQQRHWAKSIGEGVYEVNFVPPQSGVYYVFFQCPSLGIQYRQLPRLILNA
                     EAAATVTRQPAP"
     gene            5781..6539
                     /locus_tag="VGB76_13510"
     CDS             5781..6539
                     /locus_tag="VGB76_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0099960.1"
                     /translation="MTNPHASQRHQRASLKLCRIVTLIIVALCCHALDAAAQTSGAKP
                     TPQPTAQPTVQQPVQPKAQQPAAPPAAARPDPATAAQMRATAQKYFTDVELINQNGER
                     MRLYSDVLKDRVVVINAFFATCQGSCLPMNRNLEKVQAAFKERVGKDLYIVSISVDPT
                     VDTPALLKDYAKKLNATPGRLFLTGEKANVDLALRKLGQYVKQREQHTNIFIIGNERT
                     GLWKKAFGLAQPEELIKVVESVLNDAPPPTGGDK"
     gene            6539..>6807
                     /locus_tag="VGB76_13515"
     CDS             6539..>6807
                     /locus_tag="VGB76_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099961.1"
                     /translation="MRRNFRRQLRALAASLFVALAAFVYLSLPRAAAQTNASPPASTS
                     QAASSNSHAAVGGALTPEERRGKQIYLRGTSASGREIMAYLGEASL"
BASE COUNT         1400 a         2274 c         2001 g         1132 t
ORIGIN      
        1 ggcgtgatgt tccagttcga catgaagatc aacaaggccg gttggcactt cccgcagtcg
       61 cgcatcgagt cgctgtggga agactggaag ccgactatca ccggcatgcg tgcgcccgaa
      121 ccactcttct tccgcgccaa ctccaacgat tgcgtcacgt tccaccacgc caacctgatc
      181 ccgaaagtct acgtccagga tgactttcag gtgcgcacgc cgaccgacgt ggtcggccag
      241 cacatacacc tcgtcaagtt cgacgtgatg gcttcggacg gcgcgggcaa cggctggaat
      301 tatgaggacg gcacgttcgg cccgcaggag gtcgagcacc gcatcaaaca catcaacgag
      361 gtcggcggca tcttcgtgtc caaagactcc atttactctt cgcggcaacg cttactggaa
      421 atacagaagc acccctcctt cgacgcggcg acgcaggtga ccttccaacg ctggttcgtg
      481 gacgacgtga gagatcgcga cggcaacgac cgcactctgc gcaccgtctt cacgcacgat
      541 cactttggtc cttcgacgca ccagcagacg gggctgtacg ccgggcttgt cgtcgagccg
      601 aaaggttcga tctggcgtca gaacgaatcg cccggcgcac tcctgggcgg cgcgggtccc
      661 attagactgc gcgacgacgg cggccccacc agttttgcag ccgtcatcat tccgcccgac
      721 cagacgcaaa gttatcgaga gttcctgctg gagtatcagg acttccacct cgcctacgag
      781 gcgggcggcg gcggcacggc ggagaacccg gttcccgatc cggcgcgcgc catcaacccg
      841 cccggcagaa acgaaatacc tctgccgcgc atgatcatgg gtggcccgcc cttcggcccc
      901 gagacggctc tcttgaaacc gccggtcgat ggcaaatgcc ccgacggcga acccgcgccc
      961 tgcccggaga tcgtctcggc tgacgacccc ggcaccttca ccgtcaacta tcgcaacgag
     1021 ccgatcgcat tgcgcgtgcg cgacccgcgt accaacacgc aggctcccgg cgattttggc
     1081 gatctctcgc tggcctacgc gagtatcaag cggctcgacc gccgtttgaa caccgccgga
     1141 ccatacccgc cgctcacgcc cggcatgagc atctatgatc cgttcacgcc gctcctgcgc
     1201 gccaacgaaa acgaccgcgt gcaggttcgc atcctcgtgg gcgcgcacga ggaagggcac
     1261 aactttacga ttcacggcgt gaagtggttg ttcgaaccgt ccgaccccga ttcgggctgg
     1321 cgcaactcgc agatgaccgg catctccgag aagttcgact ttattctcgc cggacgcccg
     1381 cccgaagggc ctcccggcag cgagtttcgc gcagactatc tctacaaacc gggcgcagcc
     1441 gtagacgatc aatggaacgg cctgtggggc atcatgcgcg tctacaaggc cggtgcggcg
     1501 gcgcggacta acactgaaac cgactcgccg gagatgccgc agggcgacgt gccgccgttc
     1561 gagttgccga gtaatccggc cttcagaacc tcgaccgact cgacgagcca gccagaccag
     1621 gcgaccatcg cggaggaaga agcagagttt gtcggcccct gcccgcgccg cgcgcgcccg
     1681 ctgcactaca acgtggcggc ctatctggcg cgcgacatcc tgccggagcg gcgtctggag
     1741 tttaacgggc agaacggcta caccttcacc gtcacaaaca ataccaacga cccggacgac
     1801 ccttcggcgg gcgacaccga gacggggcct ttgaacgacc cgaccgccat cctcttcgtg
     1861 gatcaggaca acgacataga cccggagacg aagatgtaca agcgcaagcg cgtcgagccg
     1921 ctgctgctgc gcgccaacgc gggcgactgc atcaccgtca cgctcaccaa caacctgccg
     1981 ctggagccgg ttgatctgcc cggctataac acgatgccga tgatcgtgga ttacttcaac
     2041 gccaatcagg tcgtgccttc gcgtcgcgtt ggcctccacc cgcagttggt gcattacgac
     2101 gtgacgacga gcgacggcat gaacgtcggc ctcaacccga tccagacggt cagccccggc
     2161 gagagccgca cttacacgtg gtacgcgggg gatgtcggcg tggagttgag cacgaataga
     2221 tggtatggca agcccgtcga gttcggggcg acgaatctcg tctcgtccga tccgatcaag
     2281 cactcgaaca agagcgccat cggctcgctc atcatcgagc cgtacggcag cagttgggcg
     2341 gttgacgagg attcgacgca ctcggccatg atctacaggc cggtcgatat ctggaatgca
     2401 ccctgcgcgg gttgcccgac tcaggtggac ggtttccgcg agttcgttct gcaattccag
     2461 aacgacctca acatgcgttt tgacagtggc cgccccgtcc cgaatctggc ggaaggcgaa
     2521 gacccggaag actccgcaca gaaagcgatc aactatcgca cggagccttt ctggacgcgc
     2581 ctctgctact ggcctgcgct gcctttgacc ggcaacgacc agccgatccc tgcgcgacag
     2641 tgccccgcgt cggacgcgaa acagacgcgc gcgctcgaca tgaccaactc gctccacaac
     2701 cagcagatcg gcggcctcga tccggtcact ccgatcttca ccgccgacgc cggacaaccc
     2761 gtgcgcttcc gcgtgctcca ttcgggcggg cacgcgcgca acaacgtctt catggtacac
     2821 ggccatctct ggcaggagat gccctatcag gacggttcga actcaagcgt catcggcttc
     2881 aagcaagact cgccgtggca cggctcgcag tggggacacg gcccctcgaa ccacttcgac
     2941 gtggtgcttg aatccgccgg gggagcgttc ggggtgcagg gcgattacct gtatcgcacg
     3001 ttccagtcgt ggcagttcga cggcggcatc tggggactct tccgcgtcga gagacgtgtg
     3061 tacgaccagc ccgttgacga gatttactac tgagccatcg gttgtcctca cccgtagaga
     3121 gccgcacgcg aaaccgtgcg gctctcttcc tcaaatttaa gccgcgcccg tttcacgacg
     3181 cgcgcacgcg ccgcacccga taaggcggcg cgaaaaaaag tcgccgtcgg ccactacggt
     3241 tccggcgaca cagatgaaca gttcgatgaa agagagatga aaagagatga accagccatc
     3301 tccggcctcc gcttcatcct gcgcgccgtt tgagactccc gatctcatgc tcgccagttt
     3361 cctgcgctgc cgcagcttcc ggatcgaagc catcaaacgt caaaacggca ggactctctt
     3421 cgtcttcgac gactcgccgc acctgcgata aactctgctc gactatgcca acgacggcgc
     3481 ggtcgccgtg cgctccttct gctcgaccat gagagacttg aaggcgatca cgcgctgaca
     3541 cctgctcgca ggcgtccggt gcgcgggcac gaaatctccg aaggcgttac ggcgcgcacc
     3601 aacttcaact ccacgcgcgt cgcgccagga agcgataaaa gatcatgttg accaacacac
     3661 actggattag acgcacgctc ggaactaaac acacgttcgg aactaaacgc acgttcggga
     3721 ttagacgcgc gcttgcctcc gccctcctgc tcgccgcttt cgccagcgcg cacgcggcgc
     3781 agccgcaacc gcagaagcct tcgccccaaa cggcgcaggc gccgcagccc ggcgtgcaaa
     3841 cgaaggcgag cgcgcccgcc acggttgtcg cctcgaacgc gccgcagaag atcacgcacg
     3901 aaggcgtgga gatcgagttt tcgattgagc cgtccacgaa gtccgaaggg ctggtcgaag
     3961 gcaaggatgc gaacgtgcgt ttcaccatcc gcgacacgac gacgaagcag cccttgacca
     4021 atctccgacc ggcggcgtgg atcgatgcgc gaaaggcggg aacgacggcc accgacccga
     4081 aagcctgccg cgagaaggtg caagccttcc tccaatcgca actcacgcaa cgccccgaca
     4141 tcgatctcaa ttcctacatc atcctcgcgc tcaacgagga ggccaacatc tccgtcatag
     4201 accccctgct cggcttcggg acgaccaagc tccgcacgct cgtctttctc aaaagccccg
     4261 gtgaagattg ggtgatgagc gcagaccgca aacgcctcta cgtctcgatg ccgctcgtca
     4321 atcaagtcgc cgtcgttgac acggggagtt ggaaagttct ctcgaacata gacacgggcg
     4381 ccagacccgt gcgcgtgcga ctgcaaggcg acggaaaata tttatgggtg gcgaacgacg
     4441 ggctgggcga cgcgccgggc ggcgtcaccg tcatcgatac ggcgacgggg aaagttgccg
     4501 ccgcgcagat cgacacaggg gcggggcatc acgagatcgc tttcagcgca gacgaccgtt
     4561 tcgccttcgt cagcaacaga ctcgacggca cgctctccgt gatcgatgtc gcgcggctcg
     4621 ccaagatcaa agacctcaag acgggcgcgc agcccgcctc gctcgccttc tcgcctgcgg
     4681 gtcgcgccat ctacgtcgca cacgaaggcg acggcagcat cgtctccgta gacgccgtgc
     4741 gccatgagat tctggcgcgc gtgcaggcca aggccggact cgtcgcgctg cgtttcgcgc
     4801 ccgacgggcg gcacggcttc gtcgtcaatc aaaaagagaa cgccgtgcat atcttcgacg
     4861 cttcgacgaa tcgcctgctg cataccgtga cggtcggcaa gtcgcccgat caggtggcct
     4921 tcacgaaaca gttcgcctac gtgcgctcgc tcggccacga acaggtttcg atgatcaatt
     4981 tgtcgcatct ccagaaggga agcatcagcg aatcgtcggt ggcgcgtttc tccggcgggc
     5041 aaaagccgcc cgcgcaggcc acgagtcgtt cgacggcgga ggcggtcgtc ccttcggcgg
     5101 aagacggctc ggtgctcgtc gccaatccgg cggacaggat gatctattac tatcaggaag
     5161 gcatggccgc gccgatggga agttttacga actacaaacg cgagccgcgt tcgctcctca
     5221 tctgggacgc gtcgctgcgt gaaaccgcgc ccggcgtcta ttccaccaaa gcgcaactca
     5281 cggggcaggg caactacgac gtggccttcc tgctcgattc gccgcgcgtc gtcaattgct
     5341 tcgagatgtc ggttaaggag aaccccgaat tcgagaagaa gcgcggcctc gccatcagca
     5401 tcgagccgct cttgaaagat cgcaatctga ggacgggcga gaacgtgcag cttcgtttca
     5461 aagtgataga cacaacgacg ggtcaaccga agacggactt gaaagacatg ggcgtgctca
     5521 cgtttctctc gcccggcatc tggcagcaac gccactgggc gaagtcaatc ggcgaaggcg
     5581 tctatgaagt caactttgtg ccgccgcaat cgggcgtcta ttatgtcttc ttccagtgcc
     5641 cttcgctcgg cattcagtat cgccagttgc cgcgcctgat tctcaacgcg gaggcagccg
     5701 cgacggtgac caggcagccc gcgccctgag ccgcagcgcg cacgtcaaac agaaatattt
     5761 tccgaaaggt gagaactacc atgacgaacc cgcacgcaag ccagcgacac cagcgcgcgt
     5821 ctcttaaact ctgccgcatc gtgacgctca tcatcgtcgc gctctgttgc cacgcgctcg
     5881 acgcggcggc gcagacgagc ggcgcgaagc ccacgccgca accgaccgcg cagccgaccg
     5941 tgcagcaacc cgtgcagccg aaggcgcagc aacccgccgc gccgcccgcc gcagcgaggc
     6001 cagacccggc gactgccgcg cagatgcgcg cgacggcgca aaagtatttc acggatgtcg
     6061 aactcatcaa tcagaacggc gagcggatgc gtctctatag cgacgtgctc aaggatcgcg
     6121 tggtcgtcat caacgccttc ttcgccacct gtcagggcag ttgcctgccg atgaaccgca
     6181 atctggaaaa ggtgcaggcg gccttcaagg agcgcgtggg caaagacctc tacatcgtct
     6241 ccatcagcgt tgacccgacc gtggacacgc ccgcgctctt gaaagattac gcgaagaagt
     6301 tgaacgccac gcccggcagg ctgtttctca cgggagagaa agcgaatgtc gatctcgcgc
     6361 tccgcaagct ggggcagtac gtgaagcagc gcgagcagca cacgaacatc ttcatcatcg
     6421 gcaacgagcg caccggcctc tggaagaaag ccttcggcct cgcgcaaccg gaggagttga
     6481 tcaaggtcgt cgaaagcgtg ctcaacgacg cgccgcctcc gaccggcggc gataaataat
     6541 gcgcagaaac ttcagacgcc aacttcgcgc gctcgccgcg tctctcttcg tcgcgctcgc
     6601 cgccttcgtc tatctctcgc ttccgcgcgc ggcggcgcag acaaacgcca gcccgcccgc
     6661 ttcaacgtca caagcggcgt cgtcaaactc gcacgctgcg gtcggcggcg cgttgacgcc
     6721 ggaggagcgg cgcggcaaac aaatttacct gcggggcacg agcgcgtccg gcagggagat
     6781 catggcctat ctcggcgaag cgtcgct
//
LOCUS       DASWNS010000385         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_403813, whole genome shotgun sequence.
ACCESSION   DASWNS010000385 DASWNS010000000
VERSION     DASWNS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403813"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1897)
                     /locus_tag="VGB76_13520"
     CDS             complement(<1..1897)
                     /locus_tag="VGB76_13520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY0099962.1"
                     /translation="MKRRNRSTRSSFTALAVVLSLCLGSFGSLSARGQSSQNSKGSKA
                     AGAGAQTTGSAAARVKKPGKPLPKIAGQFDKISPDLAQQLIRSVNRKKLFRVICQFNA
                     PAGSGVQAIYTRPRVTVNYSFENSSFVSAEMPANEVFELTRFGEISYITPDREVRTLG
                     HVSTTTGADLVRAQATGGLDGTGIGIAVLDSGIERSHKSFRDGRTDDSDVRIVAEKDF
                     VSYGNSDDQYGHGTHVASLAGGNGRIANANYVGIAPNANIINLRVLDNEGRGTVSSVL
                     LALEWVRANRAAYNIRVVNMSLGMPAIDSYRHDPVCLAVRRLVDAGIVVVAAAGNNGK
                     SDDGQKVYGQIHSPGIEPSAITVGATNTFGTNERADDVVATYSSRGPTRGSYIDESSA
                     RRYDNLIKPELVAPGNKLVGAQSEGECPTSRDDCGAKNFLITQQPQMEANVSTDDSRA
                     MMFMSGTSMAAPQVAGAAALLLQVNPRLTPNMIKAILMYTAQPLAGFNMLEQGAGQLN
                     IEGAVRLAKLVRRDLTSSTPLGTPLLTGAAPAPRTTIAGHTFNWAQGVVLNYTFATGT
                     DLITDYQRIYDLGVIMSDGVIMSDGVIMSDTVPLRNPNLLSDGVIMSDTILTSDGGVL
                     GSGTPYAA"
BASE COUNT          379 a          633 c          671 g          459 t
ORIGIN      
        1 cggcggcgta aggcgtgccg ctgccgagca ctccgccgtc gctcgtcaga atggtatcgc
       61 tcatgatcac gccgtcgctc aggagattcg gatttcgcag cggcacggtg tcgctcatga
      121 tcacgccgtc actcatgatg acgccgtcgc tcatgatcac gccgaggtcg tagatgcgtt
      181 ggtaatcggt gatgagatca gttccggtcg caaaagtgta gttgaggacg acgccctgcg
      241 cccagttgaa agtgtgcccg gcgatggtcg tgcgcggagc cggagccgcg ccggtgagca
      301 gaggcgtgcc gagcggcgtg ctgctcgtta aatcacggcg cacgagtttg gcgagcctga
      361 ccgcgccttc gatgttgagt tggcctgcgc cctgttcgag catgttgaag cccgcgagcg
      421 gctgcgccgt gtacatcagg atcgctttga tcatgttggg cgtgagtcgc gggttgactt
      481 gcaggagcag cgcggcggca cccgccacct gcggcgcggc catcgaagtg ccgctcataa
      541 acatcatcgc gcggctgtcg tcggtgctca cgttcgcttc catctgcggc tgctgcgtga
      601 tgaggaaatt tttcgcgccg cagtcgtcac gactcgtggg acactcccct tccgattgtg
      661 cgccgaccaa cttgttgccg ggggcgacca gttccggctt gatcaaattg tcgtagcggc
      721 gcgcgctcga ttcgtcaatg tagctccccc gcgtgggtcc acgcgaactg taggtagcaa
      781 ccacgtcgtc ggcgcgttcg tttgtgccga aggtgttggt ggcaccaacc gtaatggccg
      841 aaggttcgat tcccggcgag tgaatctgcc cgtaaacttt ctgcccgtca tccgacttgc
      901 cattgttccc tgccgccgcc accaccacga ttcccgcatc caccagacga cgcaccgcaa
      961 ggcagaccgg atcgtggcgg taagaatcga tggcgggcat gccgaggctc atgttgacga
     1021 cgcgtatgtt gtaggccgcg cgattagccc gcacccattc gagcgcaagc aggacggacg
     1081 aaaccgtgcc ccgcccttcg ttgtccagca cgcgcaggtt gatgatgttg gcgttcggcg
     1141 cgatgccgac gtagttggcg ttggcgatgc gcccgttgcc gcccgcgagc gaggcgacgt
     1201 gtgtgccgtg gccgtactgg tcgtcagagt tgccgtagct gacgaaatct ttttcggcca
     1261 cgatgcgcac gtcgctgtcg tccgtgcgcc cgtcacgaaa agatttgtgg ctgcgctcga
     1321 tgcctgaatc gaggacggcg atgccgatgc ccgtgccgtc gagtccgccg gtcgcttgcg
     1381 cgcgcacgag gtctgcgccg gtcgtcgtgg agacgtggcc gagcgtgcgc acctctcggt
     1441 cgggcgtgat gtaggagatt tctccgaacc gcgtcaactc gaaaacctcg ttcgcgggca
     1501 tctccgcgct gacgaagctg gaattttcaa aactatagtt gacggtcacg cgcgggcgcg
     1561 tgtagatggc ctgcacaccg cttccggcag gcgcgttgaa ctggcagatg acccggaaaa
     1621 gtttcttgcg attgaccgaa cgaattaact gttgcgccaa atcgggcgag attttatcga
     1681 actgcccggc gattttcggc aacggcttgc ccggtttctt tactctggca gcagcactgc
     1741 ccgtagtttg cgcgcccgcc cctgcggctt tcgacccttt agagttttgc gaagactgcc
     1801 cgcgcgccga caggctgccg aaacttccga ggcagagaga caggacgacg gcgagggctg
     1861 taaaactgct gcgagtggag cggtttcttc ttttcatttc agtatggcgg gcggctcgaa
     1921 actctttaat ctatcgacag tatatcggga tcataagcga cgggggcaag cgttgaacgg
     1981 cgtatccttg gaagggttcg agcctggaaa agttgctgcg agccgcgcac aaacgcccga
     2041 ttttcatggc cttccggttc aatccacccc atttagcact tatacccact tatatctaag
     2101 ggcaacaatt gtgccagttg acgaaaaccg ggcgtttttg cc
//
LOCUS       DASWNS010000386         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_224163, whole genome shotgun sequence.
ACCESSION   DASWNS010000386 DASWNS010000000
VERSION     DASWNS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224163"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VGB76_13525"
     CDS             <1..1091
                     /locus_tag="VGB76_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0099963.1"
                     /translation="SNLLAAGNLDDAAAKSALETIVRNARAQRQLIDDLLDISRIITG
                     KLRLDVQPVELAPMIAAVVDGVRPAADARSIHLRMALDSHLGPISGDPDRLRQIIWNL
                     LTNAIKFTPEGGRVEIGLERTDSHVDVTISDTGQGIAPEFLPHVFERFSQSDSSSTRR
                     HGGLGLGLSIVRQLVELHGGTVTATSPGAGGVGTTFKVIFPLVNVRPELRDALVTPPL
                     SGSSPLTNPQSSLNDLQVLIVEDEPDARELVVAMMEGCGADVVAVGSAGEALEEMERQ
                     RFDVLISDIGMPVMDGYALIEKVRRLPLERGGGIPAVALTAYAGVEDRMRMLSAGYQS
                     HLPKPVEPIELTTVVANLAARHAMLVAV"
     gene            1400..1735
                     /locus_tag="VGB76_13530"
     CDS             1400..1735
                     /locus_tag="VGB76_13530"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY0099964.1"
                     /translation="MTLEKTYVEQRDGGYWVAGTRVSLDSLVYAFRRGVSPETIRRSF
                     PVLTLEEVYGAIAFYLAHEPEVDAYLEESEREFETQTKEINARTRAAKPELFERLEST
                     RRVREATRR"
     gene            1732..2082
                     /locus_tag="VGB76_13535"
     CDS             1732..2082
                     /locus_tag="VGB76_13535"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEY0099965.1"
                     /translation="MKIRFQADADLNEEIVSGILRQNPDIDFQTAEEAELRGMADPDV
                     LARAADENRILVTHDRRTMPRHFADFIGRRACPGVIIISQRVSVHRAIEELTLTWEAS
                     EAEEWSNLIIELPL"
     gene            2374..>2745
                     /locus_tag="VGB76_13540"
     CDS             2374..>2745
                     /locus_tag="VGB76_13540"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0099966.1"
                     /translation="MKDISPFSVSPNPLTLYVTDAIEAVLFKVRFTINRRQGLTVLLG
                     DNGLGKSTLVRYLHAEYDARDDVISLMTPTPSFASEFAMMQSLCGNVGLPKRRSILAH
                     QEELNAWLVEQYAAGYNVVWFI"
BASE COUNT          613 a          801 c          758 g          573 t
ORIGIN      
        1 ggtcaaattt gctcgccgcc ggcaatctcg acgacgcggc agcgaaaagc gctcttgaaa
       61 ccatcgtgcg caacgcccgc gcgcaaagac aactcatcga cgacctgctc gacatctcgc
      121 gcatcatcac gggcaaactc cgcctcgacg tgcaacccgt agaactcgca ccgatgatcg
      181 cggccgtcgt cgacggcgtg cgcccggcgg ctgatgccag aagcattcac ctccggatgg
      241 cgctcgattc acacctcggc cctatctccg gcgaccccga ccggctccga caaattatct
      301 ggaatctatt gaccaacgcg atcaagttca cacccgaagg cggacgcgtc gagatagggc
      361 ttgagcgcac ggactctcac gtggatgtga ccatcagcga tacgggacag ggcatcgccc
      421 cggagtttct gccccacgtc ttcgaacgct ttagccagtc tgactcttcg agcacgcgca
      481 ggcacggcgg gctcggactc ggtctctcca tcgtgcgcca gttggttgaa ctacacggcg
      541 ggacagtgac ggcgacgagt ccgggggcag gaggagtggg cacgaccttc aaggtcatct
      601 tcccactggt gaacgttcgt cctgaactgc gcgacgcatt ggtgacgccc cccttatctg
      661 ggagtagccc gctgaccaat ccacagtcgt cgcttaatga tcttcaggtg ctcatcgtcg
      721 aggatgagcc ggacgcgcgc gaacttgtcg tcgcgatgat ggagggatgc ggcgcggatg
      781 tggtggccgt cgggtcggcc ggcgaggcgc tcgaagaaat ggagcggcag agattcgacg
      841 tgctgatctc ggacatcggg atgcccgtca tggacggcta tgcgctgatc gagaaagtac
      901 gccggttacc gttagagcgc gggggcggaa ttccggcagt agcactgacg gcctacgctg
      961 gagtcgaaga ccgaatgcga atgctctcgg ctggctacca gagtcacctt ccgaagccgg
     1021 tcgagccaat cgaactgacg accgtggtgg cgaatcttgc cgcgcgacac gccatgctcg
     1081 tggccgttta agtaagagcg tgtgcagcga gccgactctc atgagatatt tggatcggca
     1141 tccccgctta tagcactagc ggcaatatga atcaccccct gcccgcggta gaagaccgca
     1201 ccgccctcaa gggggcattc cgtcccggct ccgactcgaa ggattcaagc cgcggtcttg
     1261 actaaaattt ttaccgtctc cgccaccggg gacatttttt gtccggccgt aggcgccacg
     1321 gaagagtctg ttgcgacagg aatgatgtta caattatctc cccgaaaggt taatattccc
     1381 gaccgaaggc agaagaacaa tgacgctgga gaaaacatac gtcgaacagc gtgatggggg
     1441 ctactgggtg gctggcacgc gcgtatcgct tgactcgttg gtctacgcct ttaggcgcgg
     1501 cgtctcgccc gagaccatca ggcgctcatt cccagtcctg acccttgaag aggtctacgg
     1561 cgccattgcc ttttacttgg cgcacgagcc ggaggttgat gcctacctcg aagaatcaga
     1621 gcgggagttc gagacccaaa ctaaagagat taatgcccgg acacgtgccg ccaagcctga
     1681 gctttttgag cgtctggaga gcactcgacg agtccgcgag gccacccgcc ggtgaagata
     1741 cgctttcagg ctgacgccga tctcaacgaa gagatcgtct caggcatact tcggcagaac
     1801 ccggacatag atttccagac ggctgaagaa gctgaactga ggggcatggc cgaccccgac
     1861 gtactggccc gtgcggctga cgaaaacaga attctcgtca cacacgaccg ccgcacgatg
     1921 ccgcgccact tcgccgattt cattggaagg cgtgcgtgcc cgggagtaat tatcatctcg
     1981 cagcgggtga gcgtccaccg agctattgag gaacttactc tcacttggga ggcgtccgag
     2041 gccgaggagt ggtctaattt aataatcgaa ctgcctttat aacctccaac ctccccaact
     2101 gtcactttca cacatctcct gaatttaagc cccatattgt ccattagcga tggattaatc
     2161 aaaagtgtgc gttgtactcc aacatgagac ggcggaactt ctcatatggg tactaagcac
     2221 tattaacttg cctacaaaat gaagagaagc agttccttgc gcatcaacca aatcagttcg
     2281 agtttctctc tgagaattat gggaagcctt aattcccttc cctgcctcaa aattagcttt
     2341 aattcttgag cgtatcatga tataaggcgc attatgaaag atatctcgcc tttttcagtc
     2401 agcccaaacc cactcaccct ctacgttaca gatgccatcg aagccgtgct tttcaaagtt
     2461 cgtttcacaa tcaatcggcg gcagggttta acggtgctac taggcgataa cggactggga
     2521 aaatccacgc ttgtgcgcta tttgcacgcc gaatacgacg cgcgcgacga tgtgatctct
     2581 ctcatgactc cgacaccgag ttttgccagt gaatttgcga tgatgcagtc gctttgcggc
     2641 aacgtcggtc tgcccaaacg ccgctcgatc ctcgcgcatc aggaagaact taatgcttgg
     2701 ctcgtcgaac agtacgcggc cggttacaac gtcgtctggt tcatc
//
LOCUS       DASWNS010000387         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_483118, whole genome shotgun sequence.
ACCESSION   DASWNS010000387 DASWNS010000000
VERSION     DASWNS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483118"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VGB76_13545"
     CDS             complement(<1..226)
                     /locus_tag="VGB76_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099967.1"
                     /translation="MINARELIGRADLLFVTLDTLRYDVAREALVQGRTPNLERALPA
                     GGWEERHTPASFTYAAHHSFFAGFLPTPTAP"
     gene            complement(223..1434)
                     /locus_tag="VGB76_13550"
     CDS             complement(223..1434)
                     /locus_tag="VGB76_13550"
                     /inference="COORDINATES: protein motif:HMM:NF038074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4014 family protein"
                     /protein_id="HEY0099968.1"
                     /translation="MWPSASERGRAHLRRVVLVGVPGSRRTELFQAALSRAGARPALV
                     VSYTDLLEGRASLTDLVRPGALVRIDSPGKDFETELALLKAGAGMEDEESRYACATRE
                     TLDALQFERGRIHWPRQWYLGFRQTLRLLETQLRCSHAHHSMNSPEEIALMFDKAACW
                     QRLSESGAPVPECLGTVDSFEELREVMRARGCARVFVKLAHGSSASGVVAYQTDGVRH
                     RASTTVEVVRRNGETILYNSRRIRVYSDAHEIAAVIDALCHHRVHVERWFPKAGIEGR
                     IFDLRVVVIAGEARHIVVRLSRHPLTNLHLLNGRGSHEALAARIGEAEWTNALHTCER
                     VMKSVLPRSLYAGIDLAVAPNFRGHRVLEVNAFGDLLPGVLHEGLDTYAAELSAAEFA
                     REQLAAATETR"
     gene            complement(1415..2356)
                     /locus_tag="VGB76_13555"
     CDS             complement(1415..2356)
                     /locus_tag="VGB76_13555"
                     /inference="COORDINATES: protein motif:HMM:NF038076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4015 family protein"
                     /protein_id="HEY0099969.1"
                     /translation="MSDYEHASEFAGRPVREWEPGTPLVDPANNAYRVGLTYEESEGG
                     ESWEKKFEALLNEPGSGRITALVVGVWGEMTGPLGNSREAVEALAAAAGRLNSLRAIF
                     LGDILAEEHEISWIEHEDVSPLFTAYPNLEHFRVRGANGLHLGTLRHDHLKSLVIESG
                     GLDASIVRDITSSQLPSLEHLELWLGVDDYGGTTEIVDLAPLFNGKLFPKLSYLGLRD
                     SEMSDAIALAVAHAPVLERLRVLDLSLGTLTDDGAAAFLSSPAAARLELLDIHHHYCS
                     DEMIEKLKAAGIKVDASEQQEPDVDGDESYRYVAVSE"
BASE COUNT          464 a          813 c          754 g          471 t
ORIGIN      
        1 cgggggcagt cggcgtcggc aggaaaccgg cgaagaagga atggtgcgcg gcgtaagtga
       61 aactcgcggg cgtgtgacgc tcttcccagc cgcccgctgg cagggcgcgc tcaaggtttg
      121 gtgtgcgccc ctggaccaga gcttcgcgcg ccacgtcgta acgcaaggtg tccagcgtca
      181 cgaaaaggag gtctgccctg ccgatcagtt cgcgtgcgtt tatcatctcg tttcggtcgc
      241 cgccgcaagt tgttcgcggg caaactctgc ggcagacaac tctgcggcgt acgtgtcaag
      301 cccctcgtgc aaaacgcccg gaagcaggtc gccgaacgcg ttgacttcga gcacgcgatg
      361 cccacggaag tttggcgcga cggccagatc aatccccgcg tagagactgc gcggcagcac
      421 gctcttcatc acgcgctcgc aggtgtgcag agcattggtc cactccgctt cgcctatcct
      481 cgccgccaac gcttcgtgac tgccgcgccc gttcagcaga tgaagattgg tgagcgggtg
      541 acggctcagg cgaacaacga tatgtcgagc ctcgcctgcg atgaccacga cgcgcaggtc
      601 gaaaattcgg ccttcgatgc cggccttcgg gaaccagcgt tcgacgtgca cgcgatgatg
      661 acacagcgca tcaatcacag cagcgatctc atgcgcgtcg ctataaacgc gtatgcggcg
      721 cgagttatag agaattgtct cgccgttgcg ccgcacgact tcaacagtgg ttgacgcgcg
      781 atgacgcacg ccgtccgtct ggtaagccac cacgcccgac gcgcttgagc catgcgccag
      841 cttaacgaag acgcgcgcac atcctcgcgc gcgcatcacc tcgcgcaact cttcgaatga
      901 gtccacagtc cccaaacact ccggcacggg cgcgccgctt tccgacagac gttgccagca
      961 ggccgcctta tcgaacatga gcgcgatctc ctccgggctg ttcatcgagt ggtgagcgtg
     1021 agagcagcgc agttgcgtct ccaacagccg cagagtttga cgaaagccga gataccactg
     1081 tcgaggccag tgtattcgcc cacgctcgaa ctgcaaggcg tcgagcgttt cgcgcgtggc
     1141 gcacgcgtag cggctctctt cgtcttccat gcccgcaccg gctttgagca gtgccaactc
     1201 cgtctcgaag tccttgccgg gagaatcgat tcgcaccagc gcgccgggac gcacgaggtc
     1261 tgtcagagac gctctgcctt caagcaggtc tgtgtaggag acgactaacg cagggcgcgc
     1321 gccagccctt gagagtgccg cttgaaacaa ctccgtgcgg cgactgccgg ggacgccgac
     1381 gagtacgacc cgccgcaaat gcgcgcgccc tcgctcactc gctgacggcc acataacggt
     1441 aggactcgtc accgtctacg tccggttcct gttgctcgct ggcgtcaacc ttgatgcccg
     1501 ccgctttgag tttctcgatc atctcgtccg agcagtagtg atgatgtatg tcgagcagtt
     1561 cgagccgggc ggcagcggga ctcgacagga aggccgccgc gccgtcgtct gtgagcgtgc
     1621 cgagtgacag gtcgaggacg cgcaaccgtt cgaggacggg agcgtgtgct acggcaaggg
     1681 caatcgcatc gctcatctcg ctgtcgcgca agcccagata cgaaagtttg ggaaagagtt
     1741 tgccgttgaa gagcggcgcg agatcgacga tctcggtcgt acctccatag tcatcgacgc
     1801 ccagccagag ttcgaggtgt tccagagacg gcagttgtga agaggtgatg tcgcgtacga
     1861 tgctggcgtc caacccgccg ctctcgatga cgagcgactt cagatgatca tgtcgcagcg
     1921 tacccaagtg gaggccgttc gcgccgcgca cgcgaaagtg ttcgaggttc gggtaagcgg
     1981 tgaagagcgg cgacacgtcc tcatgttcga tccacgaaat ctcatgctct tcggcgagga
     2041 tgtcgccgag gaagatcgcg cgcagggaat tcagcctgcc cgctgctgcg gcgagtgcct
     2101 cgacggcctc gcgcgaattt ccgagcggcc ccgtcatctc gccccacacg ccgaccacga
     2161 gcgcagtgat ccggcctgac cccggttcgt tcagcaaggc ttcaaacttc ttctcccaac
     2221 tctcaccgcc ctcggactct tcgtaggtga ggccgacgcg gtaagcgtta ttcgccgggt
     2281 caacgagcgg cgtccccggt tcccactctc tcaccggcct cccggcgaac tcgctcgcgt
     2341 gttcgtagtc cgacattgat gcctctcctc cctccctatt tttcgggttg cgctaaccgt
     2401 caaccaaatg atctttgttt actcgcagcc ccgaattcaa gcctgcgcgg ggcgctctcc
     2461 ggcgcgagtc cctacggcat tcttccgcct cgacacacgc tg
//
LOCUS       DASWNS010000388         6212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_169366, whole genome shotgun sequence.
ACCESSION   DASWNS010000388 DASWNS010000000
VERSION     DASWNS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169366"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VGB76_13560"
     CDS             complement(<1..215)
                     /locus_tag="VGB76_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099970.1"
                     /translation="MAQLDQFHETLATYRKYGWRLARVLMRTETLAELKSATGGAEFE
                     SLPAREFSLDALWFTRPAGGGREAWELR"
     gene            388..1233
                     /locus_tag="VGB76_13565"
     CDS             388..1233
                     /locus_tag="VGB76_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099971.1"
                     /translation="MRRKSENAPTDDRRTENANATDFTASPFYAALTLALKRRGVQPQ
                     ELCDANDIVERRLLVEYGAMFVVVESVRVPPRCVFRSAEECEQFQAQAEPCAAQIGEA
                     RIELQRAPLDALLEAREEARAAGFDITPRDGAEAARRTYADTLRLWESRVTPALAHWV
                     TQESLAPDEAERLGALSSREQIAAVLAEEERGNFFSKDFSRTILSSVAAPGASQHLSM
                     LAFDATEFREAGVREILARHGWFQTVRYDLPHFTFLGVREKELPALGLKRVEDCGQTF
                     WIPDI"
     gene            1281..2030
                     /locus_tag="VGB76_13570"
     CDS             1281..2030
                     /locus_tag="VGB76_13570"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0099972.1"
                     /translation="MATCASCGTNNVEGTKFCVSCGATLAPAPGSWRAGTEELQTPPG
                     SSSPTGGSSASTGSPSSSYAPHPSTPPVYSAPQPSSMYAPTAGVPGIFGQMRYAEWID
                     RVLAALIDGALTAAVMIVLFFIFGALTAASAGVGGDDNPLPGGILCLGMIVTFLSVFG
                     LGIFNKIYLVSKRGSSIGQGIMNLRVVDATGNIPPMGTLLIRLLVQVGMSFVPFISIF
                     LVLADLLWPLWDEKKQTLHDKAAGTFVVKTT"
     gene            2063..2518
                     /locus_tag="VGB76_13575"
     CDS             2063..2518
                     /locus_tag="VGB76_13575"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEY0099973.1"
                     /translation="MSNALTKWANRAGSAPGLTGALLLLQLAFVRLVASATPERAYLF
                     GRELRWDCWFKQHFGFPCPTCGMTRSVLLSLHGQFTEAAGVNPAGLLLVIGLVLFSLA
                     LIFLMFYRQRRTSLAAGAFHRRLRLGASVYGSLLVVVLFAHWLVEIASR"
     gene            2540..3100
                     /locus_tag="VGB76_13580"
     CDS             2540..3100
                     /locus_tag="VGB76_13580"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEY0099974.1"
                     /translation="MRIQFEVTPEEMVDATLRVLDRSPTYCAQRTRNVLLLAFIMAVI
                     SFFAVSFGLPGASLWAKLLVSGFAAVIGGVVQLFTERWNVERRLHAYSEERLGTDKAI
                     NFRVELTPDGILTEGQGTQVNFVWPNVEEIAETDDSIDFFMRNGSLVVVRKRAFASAE
                     EMRRFLALAAEYRARPRNFAEAVPHD"
     gene            3349..5007
                     /locus_tag="VGB76_13585"
     CDS             3349..5007
                     /locus_tag="VGB76_13585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0099975.1"
                     /translation="MDIPRVIANRFRIECEIGRGGMGTVYLATHLGLERPVAIKVLKA
                     EFAADPEVADRFMREARTMARLRHTRAAMIFDAGSLPDGRPFIVMEHVEGATLADVLA
                     REGRLSPERAVRIACEICDVLAEAHALGIVHRDLKPSNIMLNERGVSVLDFGIAKVLT
                     ASADVTKTHATTESGLIIGTPRYMSPEQCMGRPVGTASDLYSVGVLVYEMLSGQPPFI
                     DQLQSAVLVRQATAAPPPLMARCPEVPRRLALATHTLLAKNPSDRPQSARDARAMLER
                     SIQNTAQLVMPEEAAPFASTLATINTRSSNSSRVIAAATILAMLGGLFFAWSNGSTTS
                     ARSKASATRPGASSSSRAATTAAADVRTRFANDFEDEGAEAATAAVIPASLSGDAARQ
                     IAATVANDGTVGEARVLRTMRGTSIAAVHDERRRRGGTSHLFLMETRGATGGFRVAAR
                     TPLDKEGFSGAKWTTEVLDADTDGYDEIICTGTDSRDDPFSRRLVIYVPRTRQSYSMR
                     VGLDSRERQAIRVRWSSNAGGDKAKPYRAALRARALADLPQAKL"
     gene            5446..>6212
                     /locus_tag="VGB76_13590"
     CDS             5446..>6212
                     /locus_tag="VGB76_13590"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0099976.1"
                     /translation="MKPTSLMRYLSTLAILLSLAAAPLQLPASAYASARASQATARSQ
                     SAAAPQKQATQPAAAPQTQLASRVLANGLEVIVLEDHSIPLVSLELAVKNGSYTEPPE
                     LNGLSHLYEHMFFKANKAVLDEAEYLKTLDQLGILMNASTREEIVNYYLHTTSPNLRV
                     AMNYMRDAIRYPLFHERQFEQERQAVLGELDRHESNPYGFLGIELNKRLFYKYPSRKN
                     PSGNRETVTAATTEMMRLIQSRYYIPNNSAFIVTGDA"
BASE COUNT         1160 a         2100 c         1757 g         1195 t
ORIGIN      
        1 cggagttccc atgcctcgcg cccgccgcct gcgggacgcg tgaaccatag agcgtcgagc
       61 gaaaattcgc gcgccgggag gctctcaaac tctgcgccgc ccgtcgcgct tttaagttcg
      121 gcgagcgtct cggtgcgcat caggacgcgc gccagtcgcc agccgtattt gcgataggtg
      181 gcgagggttt cgtgaaattg gtcgagttgc gccatcgagt ctccgcccgg ttctcttttc
      241 gtcagtcgta actttctttt cgtcagccgc cggattttgt cgtcgtttct tttgtttaag
      301 ccgcgctctt ggtagcatac ctcacgcccg cgtccgctgc gaaaagtgag acgacacaac
      361 tcctggagac acgccgaaga atcgcgcatg cgcagaaaaa gcgagaacgc cccaacagac
      421 gaccgccgca ccgaaaacgc gaacgctacc gacttcactg cgtcgccctt ttacgccgca
      481 ctgacgcttg cgctcaaacg gcgcggcgtg cagccgcaag agttatgcga tgcgaacgac
      541 atcgttgaac gtcgcctgct ggtggagtac ggcgcgatgt tcgtggtcgt ggagagcgtg
      601 agagtgccgc cgcgttgcgt gtttcggagc gcggaagagt gcgagcagtt tcaggcgcag
      661 gccgagccgt gcgcggcgca gataggagag gcgcgcatcg agttgcaacg cgcgccgctc
      721 gacgccctgc ttgaggcgcg cgaagaggcg cgcgcggcgg gcttcgacat caccccgcgc
      781 gacggagcgg aagcggcgcg gcgcacctac gccgacacac tgaggctctg ggaatcacgc
      841 gttacgcccg cgctcgcgca ctgggtcacg caagagtcgc tcgcgcccga cgaggccgag
      901 cgtctcggcg cgctctcgtc gcgcgaacag atcgcggcgg tgctcgcaga agaagaacgc
      961 ggcaattttt tcagcaaaga tttttcgcgc acgattctct cttcggtggc cgcgccgggc
     1021 gcgtcgcagc atctctcgat gctggccttc gatgcgacgg agtttcgcga agcgggcgtg
     1081 cgcgagattc tggcgcgtca cggttggttt cagaccgtgc gctatgatct gccacacttt
     1141 acttttctcg gcgttcgtga gaaagagttg cccgccctcg gactcaagcg cgtcgaagac
     1201 tgcggtcaaa ctttctggat tcccgatatt taaccgaacc tttaaactcg aatagctcaa
     1261 cacccctgaa ggagaagaaa atggcaacct gtgcctcttg cggtacgaac aacgtcgaag
     1321 ggacgaaatt ttgcgtcagt tgtggcgcga cgctcgcgcc cgcgcccggt tcctggcgtg
     1381 ccgggactga agaattacaa acaccccccg gcagtagcag ccccacgggc ggttcttccg
     1441 cctcgaccgg ctcgccttcg agcagttacg ctccacaccc ttccacgcct cccgtctact
     1501 ccgcgccgca accctcttcg atgtacgcgc caacggctgg cgtgcccggc atcttcggcc
     1561 agatgcgcta cgccgaatgg atcgaccgcg tgctggccgc actgattgac ggcgcactga
     1621 ccgccgctgt gatgatcgtt ctcttcttca tcttcggtgc gttgacggcg gcgagcgcgg
     1681 gcgtcggcgg cgacgacaac ccgctgccgg gcggcatcct ctgcctcgga atgatcgtca
     1741 cgttcctctc cgtcttcggg ctgggaattt tcaataagat ttatctcgtc agcaaacgcg
     1801 gctcgtccat cgggcagggc atcatgaacc tgagagtcgt ggatgcgacg ggcaacatcc
     1861 cgccgatggg cactctgctc atccgcctgc tcgtgcaggt cggcatgagt ttcgtcccct
     1921 tcatctcgat cttcctcgtc ctcgcagacc tgctctggcc gctctgggat gagaagaaac
     1981 agacgctcca cgacaaggcc gcaggcacgt ttgtggtcaa gaccacttga gccgctaacc
     2041 ggataagcga cgctttaacg aaatgagcaa cgctttaacg aagtgggcta acagggcagg
     2101 cagcgcgccg ggactcaccg gcgcgctgct gctcttgcaa ctcgccttcg tgcgcctcgt
     2161 cgcatcggcc acgccggaac gcgcctatct cttcgggcgc gagttgcggt gggattgctg
     2221 gttcaaacaa cacttcggct tcccctgccc gacctgcggc atgacgcgca gcgtcctgct
     2281 ctcgctgcac ggtcaattca cagaggccgc cggggtgaac cccgcagggc tgctgctcgt
     2341 catcggactc gtgctcttca gcctcgcgct catcttcttg atgttctacc gtcaacgccg
     2401 cacgagcctc gccgccggag cgttccatcg ccgtctgcgc ctcggcgcgt ccgtctacgg
     2461 cagcctgctc gtcgtcgtcc tgttcgcaca ctggctcgtc gagatcgctt cgcgctaggg
     2521 gatacacgta agccttatca tgcgcataca attcgaggtc acgccggaag agatggttga
     2581 cgcgacgctg cgcgtgctcg accgctcccc gacctactgc gcccaacgca cgcgtaacgt
     2641 cctgctgctc gcattcatca tggcggtgat ctctttcttc gccgtctcct ttggtctacc
     2701 cggcgcgtcg ctgtgggcga aacttctcgt gagcggtttc gccgccgtca tcggtggagt
     2761 cgtccaactg tttaccgagc gttggaacgt cgagcggcgg ctgcacgcct actccgagga
     2821 acgactcggc acggacaaag ccatcaactt tcgggtcgaa ctcacgcccg acggcatcct
     2881 gaccgaaggg cagggcacgc aggtcaactt cgtctggccg aacgtcgaag agatcgcgga
     2941 gacggacgac tcgatagact tcttcatgcg caatggcagc ctcgtcgtcg tgcgcaaacg
     3001 cgccttcgct tcagcggaag agatgcgccg tttcttagct ctcgccgcag agtaccgcgc
     3061 acgtccgcgc aacttcgccg aagccgtccc gcacgactga cgcgcttttc gccgcccctc
     3121 ccgcgcaccc cgcattttgc ttttaccttt tggctctctt ggtgctaaac tccgtaactg
     3181 tccggcagtc gccgttccaa acaactgaca actttccccg ttgcctgtta gtaggattaa
     3241 caattaagtc tcctctatcg ccgctcgacc gcgctacttc cccctcgtcc ctgttcagaa
     3301 gtaaacggcc tgacgacgac cctttgtccc caaaaagagg gttttatcat ggacatcccc
     3361 cgtgtcatcg ctaaccgctt ccggatcgaa tgtgagatcg gcagaggcgg aatgggcact
     3421 gtttatctcg ccacacatct cggactcgaa cgtcccgtcg ccatcaaagt tctcaaagcc
     3481 gagtttgccg ccgaccccga agtggccgac cgcttcatgc gcgaagcgcg cacgatggcg
     3541 cgtctcaggc atacgcgcgc cgcgatgatc ttcgacgccg ggagcttgcc cgacggacgc
     3601 cccttcatcg tgatggaaca cgtcgagggc gcaacgctcg ctgacgtgct cgcgcgcgag
     3661 gggcgactct cgccggagcg cgccgtgcgt atcgcctgcg aaatttgcga cgtgctcgcc
     3721 gaagcgcacg cgctcggcat cgtccatcgc gatctcaagc cttcgaacat catgctcaac
     3781 gagcgcggcg tttcggtgct cgatttcggc atcgccaaag tgctcacggc ctccgctgac
     3841 gtgaccaaga cgcacgccac gaccgaatcg ggtttgatca tcggcacgcc gcgttacatg
     3901 tcgccggaac agtgcatggg gcgacccgtg ggcacggcca gcgatctcta cagcgtcggc
     3961 gtgctcgtct atgaaatgct gtcgggacaa ccgcctttca tagatcaact gcaatcggct
     4021 gtcctcgtcc ggcaagcgac tgccgcgccg ccgccgctca tggcgcgttg cccagaagtc
     4081 cctcgccgcc tcgctctggc gacgcacact ctgcttgcca aaaatccgtc cgaccgcccg
     4141 cagtcggcgc gcgacgcacg cgccatgctc gaacgaagca tccagaacac ggcgcagttg
     4201 gtgatgccgg aagaggctgc ccccttcgcc tcgactctcg ccacgatcaa cacgcgctca
     4261 tccaatagct cacgcgtcat cgccgccgcg acaattctag ccatgctcgg cggcctcttc
     4321 ttcgcctggt cgaacggcag cacgacgagc gcgcgcagca aagcgagcgc cacgcgtccc
     4381 ggcgcgtcgt cctcttctcg cgccgctacg actgctgccg ccgacgttcg aaccaggttt
     4441 gcgaacgact ttgaagatga gggcgcggaa gcggcgacgg ctgcggttat ccccgcatcg
     4501 ctttcgggag acgcggcgcg acagatcgcg gccaccgtcg ccaacgacgg cacggtgggc
     4561 gaggcgcgtg tgctgcgcac catgcgcggc acgagcatcg ccgccgtcca cgatgagcga
     4621 cggcggcgcg gcggcacgtc gcacctcttt ttgatggaga cgcgcggcgc aacgggaggc
     4681 ttccgcgtgg ccgcgcgcac gccgctcgac aaggaaggtt ttagcggcgc gaaatggacg
     4741 acggaagtgt tggacgcaga cacggacggc tacgacgaga tcatctgcac cggcacagac
     4801 tcgcgcgacg atcctttcag ccgccgcctc gtcatctacg tcccgcgcac gcgccagtcc
     4861 tacagcatgc gcgtcggcct cgacagccgc gaacgacagg ccatccgcgt ccgctggtct
     4921 tcaaacgcag ggggcgacaa agccaagccc taccgcgccg ccctccgcgc acgcgcgctc
     4981 gcagacctgc cgcaggcgaa actgtagctc gtccatccat ataaacgcat caaggcagac
     5041 tccatcttcg tgctatcata gccacgattg agtctgcctt ttcgcctctt gcaccgaaac
     5101 acgggtcagc aactatgtca gttcaaatcg cacgacgcca tttcttaagc gcggcgaaat
     5161 catcaactca gaaacagtcg cacaactctc tctcgctacg gatgctatcc tcggtgaaat
     5221 ttgatctgcg gcgattctca gcttgtcgcg cgccgggatt acaaaatttc gtatcctcat
     5281 cccctccatc tcatttgtca gccaagcaac gcggcgtgtt atgatcgcag tctaaagaaa
     5341 gcgaatccat agacgcctgc cagttgatgt ctctcggcca cccatgctgt ttgtcgtcgc
     5401 cagtgcacgg gcgggattcc gacgatcaat ttcatggaat aatttatgaa gccaacttcc
     5461 ctgatgcgtt atctttcaac tctggcgatt ctcctgtcgc tcgccgccgc gccgctccaa
     5521 ctgcccgcgt ccgcttacgc ctcagcccgc gcgtcgcagg cgacggcacg aagtcagtcc
     5581 gccgccgcgc cacagaaaca agcgactcag cccgccgccg cgccgcagac gcaactcgcc
     5641 agccgcgtgc tcgccaacgg actcgaagtg atcgtcctcg aagatcattc gatcccgctc
     5701 gtctctctgg aactcgccgt gaagaacggc tcttacaccg agccgcccga actcaacggc
     5761 ctctcgcatc tctacgagca catgttcttc aaagccaaca aggccgtgct cgacgaggcc
     5821 gaatatctca agacgctcga tcaactcggc atcctgatga acgcttcgac gcgcgaagag
     5881 atcgtcaact actacctgca cacgaccagc ccgaacctgc gcgtcgccat gaactacatg
     5941 cgcgatgcga tccgctatcc gctctttcac gagcgtcaat tcgagcagga gcggcaggcc
     6001 gttctcggcg aactcgaccg ccacgagtcg aacccttacg gcttcctcgg catagagttg
     6061 aacaaacgtc tcttctacaa atatccgagc cgcaagaacc ccagcggcaa ccgcgaaacc
     6121 gtgacggcgg ccaccaccga gatgatgcgt cttatccagt cgcgctacta cattccgaac
     6181 aactccgcct tcatcgtgac gggcgacgcg aa
//
LOCUS       DASWNS010000389         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_274449, whole genome shotgun sequence.
ACCESSION   DASWNS010000389 DASWNS010000000
VERSION     DASWNS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274449"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VGB76_13595"
     CDS             complement(<1..1293)
                     /locus_tag="VGB76_13595"
                     /inference="COORDINATES: protein motif:HMM:NF017043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099977.1"
                     /translation="MMIGTPLRQQYLDIKRRHPDAILLFRIGDFYEAFDDDARLIARE
                     LGIVLTSKPMGRNVRIPLAGVPYHSLERHLATLISRGHRVAICEQLTAAPVKGETGRG
                     LIERDVVRVVTPGTIIEPGLLHSKVNNYLAAYCTDGRRAGIAYADVTTGEFAATELDA
                     AAALAELQRIAPAEILVARSLQTPDADLPGFITRCNDDYFAAQLARSALLNHFEARTL
                     SAFGLSRTPLATTAAGAIIAYLRNTQTASAGQLTRLTSYHTGNFMLLDARTLRSLEVF
                     ESHGAPSLLSTLDATRTAMGGRRMRQWLRQPLLDITEIMRRHEHVGWFVEHGKARAEL
                     FAAFDFVHDLERLSGRARSGVASPQEALALGQSLETLPRVRAVLQQEQNKFGSLLAAL
                     PVCAEAARVIREAINDELPKRNEGTGIIRAGFSDELDKL"
     gene            complement(1290..>1995)
                     /locus_tag="VGB76_13600"
     CDS             complement(1290..>1995)
                     /locus_tag="VGB76_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099978.1"
                     /translation="LPILQELVATGGMDDVRYLYDRLIAYFPQIDDAEHRAKSNGELR
                     QWKRHVQRAGRELEDKRQIERRRGLWTITAQGRKRAAEEAPSFSFSLDPVATPHTGSA
                     ALSHTDVQQMLLDIGRVLGHHAESEFEYYDVVWRASESSPRLSHIFEVQRKGNVDAAL
                     AKLKRAYEAQRSKPFLIVASERDTNRANFQMNLARTGAFHEIGRVTKILSFEQLHRLH
                     RALVSVEDLLTEFIER"
BASE COUNT          337 a          578 c          690 g          390 t
ORIGIN      
        1 cagtttatcg agttcgtcgg agaagcccgc gcggatgatg ccggttccct cgttgcgttt
       61 gggcaactcg tcgttgatag cttcgcgaat cacgcgcgcc gcctcggcgc agacgggcag
      121 cgcggccagc agcgagccga atttgttctg ctcctgttgc aagacggcgc gcacgcgcgg
      181 cagcgtctcc aaactctggc cgagcgcaag cgcctcctgc ggtgaggcaa cgcctgaacg
      241 tgcgcgtccc gaaaggcgtt cgaggtcgtg cacgaagtcg aacgcggcga agagttcggc
      301 acgcgccttt ccgtgctcga cgaaccaccc gacgtgttcg tgacgacgca tgatctccgt
      361 gatgtcgagc aaaggttgac gcagccattg acgcatacgc cgaccgccca tcgccgtgcg
      421 cgtcgcgtcg agcgttgaga ggagtgaagg cgcgccgtgg ctctcgaaaa cttccaggct
      481 gcggagcgta cgcgcgtcga gcagcatgaa gttgcccgtg tgataactcg tgagtcgcgt
      541 caactgcccg gcggatgcgg tctgcgtgtt gcgcaggtag gcgatgatgg ctccggcagc
      601 ggtcgtggcg agcggggtgc gtgagagtcc gaaggccgag agcgtgcgcg cctcgaaatg
      661 gttgaggagc gcgctgcgtg cgagttgcgc ggcgaagtaa tcatcgttgc agcgcgtgat
      721 gaagccgggc aaatccgcgt ccggcgtttg caggctgcgt gcgacgagaa tctctgcggg
      781 cgcgatgcgt tggagttccg cgagcgcggc ggctgcatcg agttcggtgg cggcaaactc
      841 gcccgtcgtc acgtcggcat aagcgatacc ggcgcgtcgc ccgtcggtgc agtaggcggc
      901 gagatagttg ttgactttac tatgcagcag gccgggttcg atgatcgtgc cgggcgtgac
      961 gacgcgcacc acgtcgcgct cgatcaggcc gcgccccgtc tcgcctttga cgggcgcggc
     1021 ggtgagttgt tcgcagatgg ctacgcgatg gccgcgcgag ataagcgtgg cgagatggcg
     1081 ttcgaggctg tgatagggca cgcccgcgag cgggatgcgc acgttgcgtc ccatcggttt
     1141 cgaagtcagg acgatgccga gttcgcgtgc gatgaggcgc gcgtcgtcgt cgaaggcttc
     1201 gtagaaatcg ccgatgcgaa agaggaggat cgcgtcgggg tggcgacgct tgatgtcgag
     1261 atattgctgg cgtaggggtg tgccgatcat catcgctcga tgaattccgt gagcaggtct
     1321 tcgacggaga cgagcgcgcg atgaagcctg tgcagttgtt cgaaactcaa aatcttcgtg
     1381 acgcgcccga tctcatgaaa cgcgccggtg cgcgccagat tcatctgaaa attcgcgcgg
     1441 ttcgtgtcgc gctcgctcgc cacgatcaag aaaggtttcg agcgttgcgc ctcgtaagcg
     1501 cgcttgagtt tggcgagcgc ggcatcgacg ttgcccttgc gctgcacttc gaagatatga
     1561 ctgaggcgcg ggctgctctc gctcgcgcgc cacacgacat cgtaatactc aaactcgctc
     1621 tcggcgtgat ggccgagcac gcgcccgata tcgagcaaca tctgctgcac gtctgtgtgc
     1681 gagagcgcgg cggaacctgt gtgcggcgtc gcgacggggt cgagggaaaa agagaaactc
     1741 ggcgcttcct ctgcggcgcg cttgcgtccc tgcgccgtga tcgtccagag gccgcgacga
     1801 cgttcgatct gtcgcttgtc ttccagttct cgccccgcgc gctggacgtg tcgtttccac
     1861 tgtcgaagct cgccgttgct cttcgccctg tgctctgcgt cgtctatctg cggaaagtag
     1921 gcgatgaggc ggtcgtagag ataacgcaca tcgtccatcc cgccggtggc gacgagttct
     1981 tgcaggatcg gtaat
//
LOCUS       DASWNS010000390         5598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_455160, whole genome shotgun sequence.
ACCESSION   DASWNS010000390 DASWNS010000000
VERSION     DASWNS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455160"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1089)
                     /locus_tag="VGB76_13605"
     CDS             complement(268..1089)
                     /locus_tag="VGB76_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099979.1"
                     /translation="MNELLSIRATATANLSGRRSKGATLREFIRTQFRGEPTLAQVIF
                     DITIGMVLPVLCLVFDPLVFRGGILGRPLAGSLQFVAYGFVAIEIVALAVWLGVGSRA
                     GEWRGVLGGIMLAGALVSLVIGICLLPFSLLGLMLIIGALGFTPFITAFIYLRNARRA
                     LNAASAQLTRATLCITLLFGATLAVGTPAFAHWRVTKLVERSLAEVLGGNDVQADAAA
                     HRLRHVGWLVTAELDEMVWAYGRSKDALQQERLARAYHKITNGGDIKQRWYVLTD"
     gene            complement(1102..2301)
                     /locus_tag="VGB76_13610"
     CDS             complement(1102..2301)
                     /locus_tag="VGB76_13610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0099980.1"
                     /translation="MTNDETYDVIVVGAGLGGLQCARLLGGAGRRVLLVERKRALDES
                     VHTTGIFVRRTLEDFPLPSDCLGAAVRTVTLYSPARRALTLRSPHEEFRVGRMGKLYL
                     RFLSDCQRAGVHWLPATRYVDCEETNADGRTIVRLETGGLTRRYQTRFLVGADGARSR
                     VACDLKLDTNSEWIVGVEEVLAGVPLEGEPRFHCFVEPQLAPGYIAWVVEDGEETHIG
                     VGGYAARFNPSHALEKFRASLDGLFDLSRAKLIERRGGRIPVGGVLRRIANTRGLLVG
                     DAAGAVSPLTAGGLDPTLRLSNLAARVIDDYLETNDAASLAPYSGELFRARFGSRLLM
                     RRLFSATRQPALLELSCAALRLPGLSRLAWQVFFGRGSFPDVGSPLHAAPAPPAASPR
                     PVAPEIS"
     gene            complement(2348..4363)
                     /locus_tag="VGB76_13615"
     CDS             complement(2348..4363)
                     /locus_tag="VGB76_13615"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEY0099981.1"
                     /translation="MSNRTKLGLNILEAALLLGVLGDALLRQTPWGINVLLWTGALVI
                     AIYALHERWGRAELRRATEGVWLVLPILFFAAAFAWRDSATLKMLDGLALFVAFGLLA
                     WRARGRSIRLAGFGDYALGLGHAAFNVLFASLPLVIDDVQWATVPRGGVLRHVWAVAR
                     GVVIAVPLLFLFGMLFMAADPVFEEIMQQTFQLDPGVTFSHFFLVLLFTWLVGGFLRG
                     MMLNAEAAGEIVQAAVPAATNASYTSITIDDRERTGTDATHEGATGGHRQPPASVVVE
                     GGATAAASAATTAQATMKATTKARGFSLGAVEVGVVLGLLNVLFACFVVVQFRYFFGG
                     AARVMSITELTYAEYARRGFFELAWVAGLVLPLLLITHALLQRRDRPAAAERIFRPLA
                     GTQVALLFVIMASAMARMRLYQSEYGLTELRLYTTAFMLWLGLVFVWFTWTVLVRSRR
                     EGFACGALVAAFLTVGLLHLLNPDAYIVRANAAHARAGRAFDAAYAASLSADAVPPLI
                     SAMPSLSREERCLVARELSQRWSRSVFDEDWRAWNLARSRAWRAVAAHRPQWGDATCP
                     PPVSSVPVITPALAPETTSATFGTTTSTHAGTTNAAAPSSAPVTTTGTTNGTTNSAAT
                     MKMITETKSAAANASAAKRAKAAPRDAKNRPPKQLNRARGAKRTKAR"
     gene            complement(4557..5306)
                     /locus_tag="VGB76_13620"
     CDS             complement(4557..5306)
                     /locus_tag="VGB76_13620"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF011412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HEY0099982.1"
                     /translation="MKQRALQLAGDDSLHVAIIMDGNGRWAQERGLARTAGHRAGAEA
                     VRRAVRAAPACGIGTLTLYAFSSDNWGRPPHEVSLLLRLLGRFLDAETDELVKNSVRL
                     SVVGRRDRLRPALCAAIEKAEAKTAGGRALKLRLAVDYSSREAIMRAAKVLNSERRST
                     SMVTREEFARLLGRAVHDSAPARDVDLLIRTGGEQRVSDFLLWESAYAELYFTRRMWP
                     DFTADDLAAAVAEFHGRERRFGCVPVVAAAG"
     gene            complement(5336..>5598)
                     /locus_tag="VGB76_13625"
     CDS             complement(5336..>5598)
                     /locus_tag="VGB76_13625"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY0099983.1"
                     /translation="LRLGIVSALAGNGSLTFNELKKILQTTDGNLSVHARKLEEANYI
                     TCEKSFEGRTPKTAFQLTDDGRRALTRYLDHMESIIRTARER"
BASE COUNT         1109 a         1899 c         1686 g          904 t
ORIGIN      
        1 tccaacccaa aatgtatcct gctttttcac ctccgctcat cttagaccaa cttccggcga
       61 acccgccaac cctctcaccc gcgcgcctct catcatcgtc gctctgcaac gtctcctccg
      121 gcggcggcga aaccgcgctg ctcgtgctcg aagactaaag cctctcaaaa cgtcgaacga
      181 cgcatcaacc cgccgcccac ctttcgcgcc gctgcggtta actctgcgaa cttctccaag
      241 cggcaatctc atcaagcggc aatcgcatca atcggtgagc acataccagc gttgcttgat
      301 gtcgccgccg ttcgtgatct tgtgataggc gcgtgccagt cgttcctgct gcaacgcatc
      361 cttcgacctg ccgtaagccc ataccatttc atcgagttcg gcggtcacga gccagccgac
      421 atgtcgcagc ctgtgcgcgg cggcgtccgc ctgtacatca ttcccgccga gcacttcggc
      481 cagcgaacgc tcgaccaact tcgttactcg ccaatgtgca aaggcgggcg tgccgaccgc
      541 gagcgttgcg ccgaacagaa gcgtgatgca gagagtggcg cgggtcagtt gcgcgctcgc
      601 ggcattgagc gcgcgccggg cgttgcgcag gtagatgaag gccgtgatga acggcgtaaa
      661 ccctaacgcg ccgatgatta acatcagccc cagcaggcta aaaggcaaca ggcatattcc
      721 aataaccaga gacacgagcg cgcccgcgag catgatcccg ccgaggacgc cgcgccattc
      781 ccctgcgcgt gagccgacgc ccagccacac ggcgagcgcg acgatctcga tggcgacgaa
      841 accgtaagcg acgaactgaa ggcttccggc cagagggcgg cctaaaatcc ccccgcgaaa
      901 gacgagcggg tcgaagacga ggcagagaac cggcaacacc atgccgatag taatatcgaa
      961 gattacctgc gcgagcgtcg gctcaccacg aaattgagtg cgaatgaact cacgcagagt
     1021 cgcgcccttt gagcgtcgtc cggacagatt agctgtcgcc gtagcgcgaa tagagagaag
     1081 ttcgttcata gagtttccac ctcatgaaat ttccggcgcg acgggtcgtg gcgacgcggc
     1141 aggcggcgca ggagcggcgt gcaggggcga accgacatca ggaaacgagc cgcgcccgaa
     1201 gaagacctgc cacgcgaggc ggctcagtcc gggcaggcgc agcgcggcgc aggagagttc
     1261 gagcagggcg ggctggcgcg tcgcggaaaa gaggcggcgc atcaacaggc gcgaaccgaa
     1321 gcgcgcgcga aacagttcgc cggagtaagg agcaagcgac gcggcatcgt tcgtctcaag
     1381 gtagtcgtct atgacgcggg cggcgagatt tgagaggcgc agcgtggggt ctaagccgcc
     1441 cgccgtgagc ggcgagactg cgcccgccgc gtcgcccacg agcaggccgc gcgtattcgc
     1501 aatgcgacgc agcacgccgc cgaccgggat gcgcccgccg cgccgctcga tcagtttggc
     1561 gcgactcaga tcaaagaggc cgtcgagact ggcgcgaaat ttttccagcg catgcgaagg
     1621 gttgaagcgc gccgcgtagc cgccgacgcc gatgtgcgtc tcttcgccgt cctcgaccac
     1681 ccacgcgata tagccgggcg cgagttgcgg ctcgacgaag caatgaaagc gcggctcgcc
     1741 ttcgagcggc acgcccgcca gaacctcttc gacgccgacg atccattcac tgttcgtatc
     1801 gagtttgaga tcgcacgcga cgcgcgaacg cgcgccgtca gccccgacga ggaatctggt
     1861 ctggtagcgg cgcgtgagtc cgccagtttc cagccgcacg attgtgcgcc cgtcggcgtt
     1921 cgtctcttcg cagtcaacgt aacgcgtcgc gggcagccag tggacgcccg cgcgctggca
     1981 gtcagacaaa aatctcaagt agagtttccc catgcgaccg acgcgaaact cttcgtgcgg
     2041 actgcgcagc gtgagcgcgc ggcgtgcggg cgagtagagc gtgacggtgc gcacggctgc
     2101 gccgaggcaa tcggacggga gcggaaaatc ttcgagcgtg cgacgcacga agatgcccgt
     2161 cgtatgtacg gactcgtcga gcgcgcgttt gcgttcgacg agcagcacgc gccgacccgc
     2221 gcccccaagc agacgcgcgc actgcaagcc tcccaacccc gcgcccacca cgatcacatc
     2281 gtaagtctca tcattcgtca tcacgtttaa ctttcgtcgt gttgaacttt caccacagag
     2341 gcgcgggtca acgcgctttc gtcctctttg ctccgcgcgc ccggttgagt tgtttgggcg
     2401 gcctgttctt cgcgtcgcgc ggcgcggcct tcgcgcgctt cgcagcggaa gcgttagccg
     2461 ccgcactttt agtctccgtg atcattttca tcgtcgccgc gctgttagtc gtgccgttag
     2521 tcgtgccggt tgttgtgaca ggcgcagaag aaggagcggc ggcgttcgtc gtgcctgcgt
     2581 gtgtagaggt cgtcgtgccg aacgtcgcgc ttgtcgtctc cggcgcgagg gcgggtgtaa
     2641 ttacgggaac actgctaacg ggcggcgggc aggtggcgtc gccccactgc gggcgatgcg
     2701 cggcgacggc gcgccacgca cgcgagcgcg ccagattcca cgcgcgccag tcttcatcga
     2761 acacagagcg gctccagcgt tgcgaaagtt cgcgcgccac aaggcaacgc tcttcgcggc
     2821 tgagggaggg catggcggag atgagcgggg gcacggcgtc ggcactcaac gacgccgcat
     2881 acgctgcatc gaacgcgcgc cccgcccgcg cgtgcgcggc attggcgcgc acgatatagg
     2941 catccggatt gaggagatgc agaagcccta cggtgaggaa cgcggcgacg agcgcgccgc
     3001 acgcaaagcc ttcacgacga ctgcgcacga gcaccgtcca ggtgaaccag acgaagacga
     3061 ggccgagcca gagcatgaaa gcggtcgtgt agagacgcaa ctccgttaag ccgtactcac
     3121 tctggtaaag ccgcatgcgc gccatcgccg aagccatgat gacaaagagg agcgcgacct
     3181 gcgttccggc cagcgggcga aaaattcgct ccgcagcggc ggggcgatcc ctgcgctgta
     3241 agagcgcgtg cgtgatgagc aggagcggaa gcaccagccc cgccacccac gccagttcga
     3301 agaagccgcg ccgcgcatat tccgcgtagg tcaactcggt gatggacatg acgcgcgccg
     3361 cgccgccgaa gaagtagcga aactgcacga cgacgaaaca ggcgaacaac acgttcagca
     3421 aaccgagcac gacgccgact tcgactgcgc ccaatgaaaa accacgcgcc ttcgtcgtcg
     3481 ctttcatcgt cgcctgcgcc gttgtcgctg ccgacgccgc tgccgtcgct ccgccttcga
     3541 caacgacgct cgcgggcggt tgcctgtgcc cgcccgtcgc gccctcatgc gtcgcgtccg
     3601 ttccggttcg ctctctgtcg tcaatggtga tcgaagtgta ggacgcgttc gtagcggcgg
     3661 ggacggcagc ctgcacgatc tcgcccgccg cttctgcgtt cagcatcatg ccacgcagga
     3721 agccgcccac gagccacgtg aacagaagca ccaggaagaa gtgcgagaac gtcacgccgg
     3781 ggtctaactg aaacgtctgc tgcatgatct cttcaaagac cgggtcggcg gccatgaaca
     3841 acatgccgaa gaggaacagc agaggcacgg cgataaccac gccgcgcgcc accgcccaca
     3901 cgtgtcgcag cacgccgccg cgcggaaccg tcgcccactg cacatcgtcg atgaccagcg
     3961 gcaaactcgc gaacagcaca ttgaaggctg cgtgtcccag ccccagagcg taatcgccga
     4021 agcccgccag cctgatgctt cgcccgcgag cgcgccacgc cagcagcccg aacgcgacga
     4081 agagcgccag cccgtcgagc atcttcaacg tcgccgaatc acgccacgcg aaggccgccg
     4141 cgaagaagag gatgggcagc accaaccaca cgccctccgt cgcgcgccgt aactccgcgc
     4201 gcccccaacg ctcatgcagc gcatagatcg cgatgacgag cgcgcccgtc cataacaaga
     4261 cgttgatgcc ccacggcgtc tggcgcagca gcgcgtcgcc gagcacgccc aacaacaacg
     4321 ccgcttccag aatgttcaaa cccaacttcg tccggttgct catcgcgcgc ctcctaccga
     4381 atttgttcct gccgtcgtta cacgttgccg ccgcacactt gttccaacat ccaactcgac
     4441 ggcacgccgc cctcagcttc gacctttcaa acgtctcaac ctctgcctct tcaaactctt
     4501 caacctccgc acagcgtcgc tctcaaattc acacggagcg acacaacccg cgcgcttcaa
     4561 cccgccgccg cgacgacggg cacacaaccg aagcggcgtt cgcgcccgtg aaactccgcg
     4621 acggctgccg ccagatcgtc cgcagtgaaa tccggccaca tccgccgcgt gaaataaagt
     4681 tcggcatacg cgctctccca caacagaaaa tcgctcacgc gctgttcgcc gcccgtgcga
     4741 atcaacaggt ctacatctcg cgcgggcgcg ctatcgtgca ccgcgcgacc cagcaagcgc
     4801 gcaaactctt cacgcgtcac catgcttgtc gagcgtcgct ccgagttcaa cacttttgcc
     4861 gcgcgcataa tcgcctcacg cgatgaataa tcaaccgcga gccgcaactt aagcgcacgc
     4921 ccccccgcgg ttttcgcttc cgccttttcg atggcggcgc acaacgccgg acgcaggcgg
     4981 tcgcgccgcc cgacgacgct caggcgcacg ctgtttttga caagctcatc cgtctccgcg
     5041 tcgaggaagc gaccgaggag tcgcagcaag agcgagactt cgtgcggcgg tcgcccccag
     5101 ttgtccgagg agaacgcgta gagcgtcagc gtcccgatgc cgcaagcggg cgcggcacga
     5161 accgcgcggc gtaccgcttc cgcccccgcg cggtgtcccg ccgtgcgcgc cagtccacgc
     5221 tcctgcgccc aacgcccgtt gccgtccatg atgatcgcga catgcaggct atcgtcgccc
     5281 gccaattgca aagccctttg tttcataaag tcaccggaca aaaaaatttg acctttcagc
     5341 gttcccgcgc tgtgcggatg attgattcca tgtgatcgag atagcgtgtg agcgcgcgtc
     5401 gcccgtcgtc cgtcaattga aaggcggtct tcggcgtacg cccttcgaac gacttttcac
     5461 aggtgatgta gttggcctct tcgagtttgc gcgcgtgcac gctcaggttg ccgtccgtgg
     5521 tctgcaaaat ctttttgagt tcgttgaagg tgagcgagcc gttgcccgcg agcgcgctga
     5581 cgataccgag ccgcaggc
//
LOCUS       DASWNS010000391         9770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_41141, whole genome shotgun sequence.
ACCESSION   DASWNS010000391 DASWNS010000000
VERSION     DASWNS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41141"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..1605
                     /locus_tag="VGB76_13630"
     CDS             412..1605
                     /locus_tag="VGB76_13630"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0099984.1"
                     /translation="METQKKITETVFLDPDQKSPRAQEFEEKLSTRIVGQERAVRRMS
                     GLYQIFLAGMNPLNRPVGTMLFLGPTGSGKTRVVEAAAEVLFGDPGAVIKIDCAEFQH
                     SHEIAKLIGSPPGYLGHRETSPMLTQENLDRYHTEDTKLSLVLFDEIEKASDSLWQLL
                     LGILDKATLTLGDNRRVDFSHTMVIMTSNLGAREMSELISGGMGFVPTHGAKNPNDSD
                     VDQKIYRTAVEAARRKFSPEFMNRIDKVVVFRSLKEHHLRQILELELASVQDRIMRSA
                     GTKFVFQCSDAAKDMLLKEGIDYKYGARHLKRSIERFLVYPLSNLVATDQVGLGDLVY
                     VDLLDGQNKLVFSKRSGGALISDVAPAEETFVETEAKSGGVALPIPQTKVARKSQGRG
                     EKSES"
     gene            complement(1852..2769)
                     /locus_tag="VGB76_13635"
     CDS             complement(1852..2769)
                     /locus_tag="VGB76_13635"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0099985.1"
                     /translation="MKRTGARSFSEPILLDDARRRAWLCAVLSAALFFVATSRAEAAP
                     LHACANTFPQVTQSTLTPLQREIEKQRARLASGDVEERREAVMRLGSMARADSSRAAL
                     PALRDAAVIVRATAARAVLSLPAGEAAAALLPLLSDKDEFVRRETAYALGETRSRAAV
                     VALATALARDKEAGVRGAAAVALGQIGDETAVPVLTEAIGRRIARSGFLNRITFRRTE
                     ENEFVRRSAAVSLGQIKSRAAVPALIGALANERAGDDVRREAARALGLIGDPSAIPTL
                     QRALLTARDPYLSEIASEAIRKLEPGKAG"
     gene            complement(2766..3032)
                     /locus_tag="VGB76_13640"
     CDS             complement(2766..3032)
                     /locus_tag="VGB76_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099986.1"
                     /translation="MAKRITERQRLAFKLESLTDTEVKEVLDYISIMESMRRATSPGG
                     LWDDELVTLLADARENLRARQAFEWESVRRRADRRADALTPTRV"
     gene            complement(3484..4284)
                     /locus_tag="VGB76_13645"
     CDS             complement(3484..4284)
                     /locus_tag="VGB76_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY0099987.1"
                     /translation="MVDYYKILGIQRNASASEVKSAYRRLARQRHPDLNGGSEKAARE
                     FALIALAYRTLSDPQERTYYDNQFRRITSDASGSFLHSENPHARRMRRVAAQARMDRV
                     VDNLIEAERRETFAMQRAVFTTVSLFLSTFFVALFKPRFWQLFGGVGRALMLTLFLIG
                     LWHLISRLREYFNRYTYQPSSLHDSVTHEVEKSDKPFTRFAASSFLVIGYMACLGAGL
                     LLGEQMYSTILSEVPFLYDTQIRPELLFYPPIAVLVIDTLHMIAVKID"
     gene            complement(4539..5090)
                     /locus_tag="VGB76_13650"
     CDS             complement(4539..5090)
                     /locus_tag="VGB76_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099988.1"
                     /translation="MRKQITMFVASLALLLGSGTFLPQVKANASTKETAVVIKLTLPD
                     GTWVEARVIEDGLLKLEDEKTGQTTGFSLKVLNKSKGTVKITVLDLADGSNGSFREVE
                     SLEASFKSPKTTSVFPATTIEILAVVEQIPEKASTRGRGETVSLAPSNCCIRCEGKTL
                     CANCYVQMSCGCCNTDLCPGSCS"
     gene            complement(5557..7131)
                     /locus_tag="VGB76_13655"
     CDS             complement(5557..7131)
                     /locus_tag="VGB76_13655"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY0099989.1"
                     /translation="MDIQVSTGRVGDAEVQALAVAVFKDEQAHDGFLQELDAATGGLI
                     KSVIESEELTGKEGETVYVHLGDAREGLKAKRLLLVGVGEREGYKTAQVSQMAGTAAR
                     ALRARNVKSVGLITRATGSDVEQTAAAAVEGVLMALFEPDKYRTSDKEERLIERLVII
                     TQWASEEALRRGAERGRIIGECVNFTRDLANEPGAYMTPTIMAERARAIASEFNLEVD
                     ILDRERMEGLGMGSLLSVARGSEEPPALIILKYTPEGFNGKAEGGVEGSGGNTDGLLA
                     FVGKGVTFDSGGISLKPGENMELMKYDMTGGATVLGVMRAVAQLKPPMPLLGVVPATE
                     NLPSGKATKPGDVVRAMSGKTIEVINTDAEGRLILADAIAYAKKLGATRIVDLATLTG
                     AVSIALGDVNTGILGTDQELIDEVIAAGREVGEKLWQLPLDKEYTKQIKSDIADIKNV
                     GGRKAGTITAAAFLKEFADGVSWAHLDIAGTAWGDDAKPYRSKGPTGVGVRTLLNLIE
                     RAVAARAASEGKGGKA"
     gene            complement(7151..7618)
                     /gene="smpB"
                     /locus_tag="VGB76_13660"
     CDS             complement(7151..7618)
                     /gene="smpB"
                     /locus_tag="VGB76_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY0099990.1"
                     /translation="MTEADGVKVLLTNRSAFHEYHILNTYEAGVVFKGTEVKSVRNGQ
                     IQLKESYVAVREGEVWLFNAHISPYAYGNRDNHEATRTRKLLLHRREIDKLEQEATVK
                     GMTLIVTRVYLKKGRIKFEVGVARGKKLYDKRETEMRRTVDRETRAALKERNR"
     gene            complement(7676..8695)
                     /locus_tag="VGB76_13665"
     CDS             complement(7676..8695)
                     /locus_tag="VGB76_13665"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY0099991.1"
                     /translation="MMSQIAIIGAGGWGTALGMVAGRAGHAVRLWSRNAEVVAGINER
                     RLNGVYLAAHELEGDVRATGELGEAVGDAQLVILAAPSHATRELLVQTARYLRPEVVL
                     VSATKGIEIETGQRISEVAEEVLGRELTVRFVCLSGPSFAQEVAAGQPTAVVAAGYDA
                     RATLAVQAALSHENFRVYTNGDLVGTELGGAVKNVIALASGMVAGLGLGTNSVAALIT
                     RGLVEITRLALAEGGRVETLAGLAGLGDLVLTCTGGLSRNRYVGQELGRGRALEEILR
                     GMREVAEGVRTTRAVKLLADNTGIEMPITEQVHAVLYEGKSVRAAAEELMARPPRGEF
                     SGMGL"
     gene            complement(8778..9401)
                     /gene="plsY"
                     /locus_tag="VGB76_13670"
     CDS             complement(8778..9401)
                     /gene="plsY"
                     /locus_tag="VGB76_13670"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY0099992.1"
                     /translation="MLPALFIVAAYLTGSIPFGYLIVRARGGGDVRETGSGGTGATNV
                     TRRAGRSAGILTLALDAAKGALVALTARWLLAPDFGIDEWVAAASIAVVVGHVFPVWL
                     RFRGGKGVATGLGVFLSLTPAAVALAVPVFIAVVWATRYISLGSIMATATLPLFVWLL
                     SERAKSDGDAAFAPLMAVAIIGGALIIFMHRANIGRLLNGTENRWKG"
     gene            complement(9525..>9770)
                     /locus_tag="VGB76_13675"
     CDS             complement(9525..>9770)
                     /locus_tag="VGB76_13675"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY0099993.1"
                     /translation="PVAEAMAEGVRERAGTDFGLSVTGIAGPGGGTEDKPVGLVYLAL
                     SDDAHTEHRKLLLPGDRQLIRWRASQAALDLLRRRLI"
BASE COUNT         2000 a         3005 c         2752 g         2013 t
ORIGIN      
        1 gttttcactg ttcgagccgc cgacgcgccc tgctttcggg cttatacctc ttggtgcgtc
       61 aagcttaaat tttgcacgaa gagagtaaga agacagtaat ttgtcagttg cgttctgccg
      121 ctaattgtca gtgagtgaaa attgtcgggg cgcggaaaaa gttttggcgc ggctcatatt
      181 ttgtggtgac aaatcgctca ttgtcgttta tatagtgttc tcactctcga acgccggaga
      241 gaacgcggcg caaattagcc aaccggcacc gtgcccggcg catcaattac acgaacggat
      301 cgttctttgc atgaggacgg aggtgtggcg gtcaagcgac tgccggagca gctaattcaa
      361 gctcaccccc gaaacgctca ggcaaggagg acggtcgcga tgatggcagc aatggaaaca
      421 cagaagaaaa taaccgagac ggtctttctt gaccctgatc agaaaagccc gcgcgcacag
      481 gagtttgaag aaaagctctc gacgcgcatt gtcggccagg agcgtgcggt gcgccgcatg
      541 agtggcctct atcaaatttt tctcgcgggc atgaacccgc tcaaccgccc cgtcggcacg
      601 atgctgtttc tcggtcccac gggatcgggc aaaacgcgcg tcgtcgaggc ggctgccgaa
      661 gttctcttcg gcgaccccgg tgcggtcatc aaaattgact gcgcggagtt tcagcattca
      721 cacgagatcg cgaagttgat cggctcgcct ccgggctatc tcggccatcg cgaaaccagc
      781 ccgatgctga cgcaggaaaa cctcgaccgt tatcacacgg aagacacgaa actctcgctc
      841 gtcctcttcg acgagatcga gaaggcttcc gattccctgt ggcaactcct gctcggcatt
      901 ctcgacaagg ccacactcac gctcggcgac aaccgccgcg tggatttctc gcacacgatg
      961 gttattatga cttcgaacct cggcgcgaga gagatgtcgg agttgatctc cgggggcatg
     1021 ggttttgtgc cgacgcacgg cgcgaagaat ccgaacgact cagacgttga ccagaagatt
     1081 tatcgcacgg cggtcgaagc ggcgcggcgc aagttctcgc cggagtttat gaaccgcatc
     1141 gacaaggtgg tcgtgttccg cagtctcaag gagcatcacc tgcgccagat tctcgaactc
     1201 gaactggcat ccgtgcagga tcgcatcatg cgttccgccg ggacgaagtt cgtctttcaa
     1261 tgttccgacg cggcgaagga catgctgctc aaagagggca ttgattacaa atacggcgcg
     1321 cgacatttga agcgttcgat tgaacgtttc ctcgtctacc cgctctcgaa tctggtggcg
     1381 acggatcaag tcggactcgg cgatctggtt tacgtagact tgctcgacgg ccagaacaag
     1441 ctcgtctttt cgaagcgttc gggcggcgcg ctaatcagcg atgtggctcc ggcggaggaa
     1501 accttcgtgg aaaccgaggc caagtcaggc ggcgtggcgt tgccgatccc gcagacgaag
     1561 gtggcgcgga agagtcaggg acgcggcgag aagtcggaaa gctaaaagcc gactcaaact
     1621 acagaacatg aaagccggag cgcgagtttt atcgcctccg gcttttcgtc tgcgtcaact
     1681 tcttctcgct tggctgaggc ttcaacaaat actcgtcgca gagatgctgg agcggactcg
     1741 ccgcaacggt agatgaaggc gacgctattt aaaatttttc cgtgctttct ctcgcgtcat
     1801 ccgcgtctct gcggcggcga acgtctcggt cgagttaagt cgcaaacgat gtcagccggc
     1861 ttttcccggt tcgagtttgc gtatggcttc ggaagcgatc tccgagagat aaggatcgcg
     1921 tgcggtgagc agggcgcgct gtagcgtggg aatggcggag gggtcgccga tcagaccgag
     1981 cgcgcgtgcg gcttcgcggc gcacatcgtc tccggcgcgt tcgttggcga gcgcgccgat
     2041 gagcgcgggc acggcggcac ggcttttaat ctggccgagc gagacggcgg ctgaccgtcg
     2101 cacaaactcg ttctcttccg tgcgtcgaaa cgtgatgcga ttcaggaaac cggaacgcgc
     2161 gatgcggcga ccgatggctt cggtcaaaac cgggacagcc gtttcgtcgc caatctgtcc
     2221 gagcgcaacc gcagccgctc cgcgcacgcc cgcttctttg tcgcgcgcca gtgcggttgc
     2281 gagagcgacg accgccgcgc ggctgcgcgt ctctcccagc gcgtaggcgg tttcgcggcg
     2341 cacaaattca tccttgtcgg agagcaacgg gagcagcgcg gcggcggctt ccccggcggg
     2401 cagagacagc acggcgcgtg cggcggtggc gcgcacgatg acggcggcat ccctgagcgc
     2461 gggcagagcg gcgcgtgaac tgtctgcacg cgccatcgag ccgagccgca tgacggcctc
     2521 gcgcctttct tcaacgtcgc cggaagcgag gcgcgcgcgc tgtttttcga tttcgcgttg
     2581 aagcggagtt agagttgact gtgtgacttg cgggaaagta tttgcacacg cgtggagagg
     2641 cgctgcctcc gcgcggctcg tagcgacgaa aaaaagcgcg gcagagagca cagcgcacag
     2701 ccaagcacgc cgccgtgcgt cgtcgagcag gataggttcg gagaacgaac gcgcgcccgt
     2761 tcgcttcata cgcgggttgg ggtcagggcg tctgcgcgac ggtcggcgcg gcgtctgacc
     2821 gattcccatt cgaaggcttg ccgcgcacga aggttttcgc gtgcatccgc gagcagtgtg
     2881 acgagttcgt cgtcccacaa cccgccgggg ctggtcgcgc ggcgcatcga ctccatgatc
     2941 gagatgtagt cgagcacttc tttaacttcg gtgtcggtca gggattcgag tttaaaagcg
     3001 aggcgctggc gttcggtgat tcgcttagcc atctcttgct cctccagtct ccgagaagcc
     3061 ggacggccaa agcgcgcatc aacccgggag acgagttgtg cagcgacgtt cgggaggccg
     3121 tcggctgttt gtgattagca cgaggttgtg ttgtgcgaaa gttactttga ttctcacctg
     3181 ctgaatgtgt ctgtttcgcc agcgagccac gccgcttgac gcagcccggc tcacgtttta
     3241 agggggaagc acacaacaga cgttaatccg ctctaacaac ggataaggct ccggctcttg
     3301 ccgtcgtcct gcgttgtgcg cagctaacta aataacgatt gtgcgaattt gcttcaataa
     3361 cggctttttc gttggccgct ggaggcgaaa tatacacgag acgcatcgcc ggacgcaaga
     3421 ctgcccgaga cgcacaagca cccgcttgcg cgtcttgccg ggaattgacg aagcggcaag
     3481 tggtcagtct attttgacgg cgatcatgtg cagcgtgtct atcacgagca cggcgatggg
     3541 cgggtagaag agcaactccg gacggatttg ggtgtcgtag aggaacggca cttcgctcaa
     3601 gatggtgctg tacatctgct cgccgagcaa gagcccggct ccgaggcagg ccatgtaacc
     3661 gatgacgaga aaactcgatg cggcgaagcg tgtgaaaggt ttgtccgact tttcgacttc
     3721 gtgcgtgacc gagtcgtgca gcgaactcgg ctggtaagtg taacgattga aatattcgcg
     3781 caggcgagag atgagatgcc acagcccgat caaaaacagg gtgagcatca gggcgcgccc
     3841 cacgccgcca aacaattgcc agaagcgcgg tttgaacagc gcgacgaaga aggtagagag
     3901 aaacagggag acggtggtga agacggcgcg ctgcatggcg aaggtttcgc gccgctcagc
     3961 ctcgatcaaa ttatctacca cgcgatccat gcgcgcctgc gcggcgaccc ggcgcatccg
     4021 gcgcgcgtgc gggttttcgg aatgcaaaaa cgaaccgctc gcgtcgctcg tgatgcggcg
     4081 aaattgattg tcgtaataag tgcgctcttg cgggtcgctg agcgtgcgat aggccagcgc
     4141 gatcaaggca aactcgcgcg cagctttctc cgaaccgccg ttgaggtcgg ggtgtctctg
     4201 gcgtgccaga cgcctgtatg ctgatttgac ttccgaggcc gaagcgttcc gctggatgcc
     4261 taaaatttta taataatcaa ccactgcgct ttgtcccgta ctccgtaaaa gccagcgtgg
     4321 ggcgcaaata tccgtaaatg cgccccgggc aggaagggtc aacccgtagc gacactatag
     4381 cacttttcta aaaaaatctc ctgtcagaag ttattgagcg cgtgtaacaa aagttatgct
     4441 gaagctacgc tcacctcgat tacttatact gcaactgtta aaaaaaggag cgttgcctcg
     4501 gggacaacgc tccttttttt aaggctagtt tgtaatgttc aactgcaact gcctgggcaa
     4561 aggtcagtgt tgcagcagcc acaagacatt tgaacgtagc agttcgcaca aagcgtttta
     4621 ccctcgcacc taatacaaca gttagatggc gctagactaa cagtttctcc tcgaccccta
     4681 gttgatgctt tctcaggaat ttgttcgacc acagctaaaa tttcaatggt cgtcgccgga
     4741 aagacggaag ttgttttcgg ggatttgaat gatgcttcta aagattccac ctctctaaaa
     4801 gagccattgc taccgtctgc caagtcgaga actgttattt tcaccgtccc ctttgactta
     4861 ttcagaactt tcaaagaaaa gcccgtggtt tggccggttt tttcatcttc aagtttgagc
     4921 aagccatctt cgatcacgcg ggcttcaacc catgtgccat ctggtaaggt gagtttaatc
     4981 accacagccg tttctttagt agaagcattg gctttcactt gcggcagaaa agtcccgctg
     5041 cctagcaaaa gggcaagact agctacaaac atggtgattt gctttctcat gttcatacaa
     5101 gttttccttt caagagtaga aagttgccac ctcgatttgc gttatgccac ggcatttcgt
     5161 gaggctggca aaactgtcgt ggtgggggaa ggcgtgttaa atatagagga agtcaaaggg
     5221 aggggtgctt tacctcggac ggccaccgag tgggcaagga cagttatttg tgttaccgct
     5281 ccctttgact tggcaccttt gccagagcaa tgtttatgcc agaggcagac aataccagaa
     5341 tcggcgaaaa atgcaagttt tttagcagag actattaact ataatgtcca ctaaagtaga
     5401 cggcgcgtct acaatagtgg acgacccgga gacgcaaagc gcacatttca gccgcttctc
     5461 aaaaattctc ctccccaaaa acagaaggcc gcgccggttt atctccgcgc gacctctgtt
     5521 gtttgcgacc tcaaatgctc gtcaaaacgt ctgatcttaa gccttgccgc cttttccttc
     5581 ggacgcggcg cgtgctgcga cggcgcgctc gatcaagttg agcagcgttc ggacgcccac
     5641 gcccgtcggc cctttagagc gataaggctt ggcatcgtcg ccccacgccg tgcccgcgat
     5701 gtcgagatgc gcccacgaaa cgccgtcggc aaattctttg aggaaagctg ccgccgtgat
     5761 cgtcccggcc ttgcgcccgc cgacattctt gatgtccgcg atgtccgact tgatctgctt
     5821 cgtatactct ttatcgagcg gcaactgcca gagtttctca ccgacttccc tgcccgccgc
     5881 gataacttcg tcaatcaact cctgatccgt gccgagaata cccgtgttga catcgccgag
     5941 tgcgatggag actgcgcccg tcaacgtcgc gagatcgacg atgcgtgtgg cgccgagttt
     6001 cttcgcgtag gcaatggcat cggccagaat caagcggcct tcggcgtccg tgttgatcac
     6061 ctcgattgtc ttgcccgaca tggcgcgcac cacgtcgccc ggcttcgtgg ccttccccga
     6121 aggcagattc tccgtcgcgg gcacaacgcc gagtaagggc atcggcggct tcaactgcgc
     6181 caccgcgcgc atcacaccga gcaccgtcgc gccgcccgtc atgtcgtact tcatcaactc
     6241 catgttctcg cccggcttga gcgagatgcc gccggaatcg aacgtcacgc ctttgccgac
     6301 aaacgcgagc aggccatccg tgtttccgcc gctgccttcg acgcctcctt cagctttgcc
     6361 attgaagcct tcgggcgtgt acttcaagat gatgagcgcg ggcggctctt ccgaaccgcg
     6421 cgccacgctc aggagcgacc ccatccccaa gccttccatg cgctcgcgat ccagtatgtc
     6481 aacttccaga ttaaactcgc tcgcaatcgc gcgtgcgcgc tcggccatga tggtcggcgt
     6541 catgtacgcg ccgggttcgt tggcgagatc gcgtgtgaag ttaacgcact caccgatgat
     6601 gcgcccgcgc tccgcgccgc gccggagagc ttcttcgctt gcccattgcg taatgatgac
     6661 gagccgctcg ataaggcgtt cctctttgtc ggacgtgcga tatttgtccg gctcgaacaa
     6721 tgccatcaag acgccttcga ccgccgctgc cgcagtctgc tcaacgtcgc ttcccgtcgc
     6781 acgcgtgatc agcccgacgc ttttaacgtt tcgcgcacgc agcgcacgcg cggcggttcc
     6841 ggccatctgc gacacctgcg ccgtcttgta gccctcgcgt tcgccgacgc cgacgagcag
     6901 caaacgcttc gccttcaatc cttcgcgcgc gtcgccgaga tgcacataaa ccgtctcgcc
     6961 ctctttgccc gtgagttctt ccgactcgat cacggatttg atcaggccgc ccgtcgccgc
     7021 gtccaactcc tgcaaaaatc catcgtgcgc ctgttcgtct ttgaaaactg ctacggcgag
     7081 agcctgcact tctgcatcgc ccacgcggcc tgtgctcact tgaatatcca tattaacttt
     7141 tatctcccgg ttaacgattg cgctctttca gcgcggcgcg tgtttcgcga tcaaccgtcc
     7201 gacgcatctc cgtctcgcgc ttgtcataaa gttttttgcc gcgcgcaacg ccgacttcaa
     7261 acttgatccg cccttttttc agatagacgc gggtgacgat gagcgtcatg cctttcaccg
     7321 tcgcctcctg ctccaattta tctatctcac gtctgtgtaa caaaagtttc cgcgtgcgcg
     7381 tggcttcgtg attatcgcga ttgccgtaag catacggcga aatgtgggca ttaaacaacc
     7441 agacttcgcc ctcgcgcacg gccacgtaag attccttcaa ctgaatctgc ccgttgcgca
     7501 cgcttttaac ttccgtgccc ttaaagacga ctcccgcctc gtaagtgttc agaatatgat
     7561 attcatgaaa cgccgagcga ttcgtcaaca acacttttac tccgtccgct tctgtcatct
     7621 tcctaacttt ctcaagcgct cgctcctgcc aaatgcgcgt cacaattttt tcggctcaca
     7681 gccccatgcc ggaaaactcg ccgcgcggag gccgcgccat caattcttcc gccgccgccc
     7741 gcacgctctt gccttcgtag agcaccgcat gcacctgctc cgtgatcggc atctcgatgc
     7801 ccgtgttatc ggccagcaac ttcaccgcgc gcgtcgtgcg caccccctcg gctacttcgc
     7861 gcatcccgcg caaaatctct tcgagcgcac gcccccgccc caactcctgc ccgacataac
     7921 gattgcgcga cagcccaccc gtgcacgtca aaaccagatc gcccaacccc gcgagtcccg
     7981 cgagcgtctc gacgcgccca ccctcggcca gagccagccg cgtgatctcg accaagcccc
     8041 tcgtgattaa agccgcgacg ctgttcgtgc ccaagcccag acccgcgacc atccccgacg
     8101 ccagcgcgat gacgttcttt accgcgccgc cgagttccgt ccctacgagg tcgccgttcg
     8161 tatagacacg aaaattctca tgactcaaag ccgcctgcac ggccagcgtc gcacgcgcat
     8221 cgtaaccggc ggcgaccact gccgtcggct gtccggctgc cacctcctgc gcaaacgatg
     8281 gacccgacag gcagacgaag cgcaccgtca actctctgcc cagaacctct tccgcaacct
     8341 ccgagatgcg ctgtcccgtc tcgatctcga tgcccttcgt cgcgctcacg agcactactt
     8401 cgggacgcaa gtatcgcgct gtttgcacca gcaactcgcg cgtcgcgtgt gaaggcgcag
     8461 ccagtatcac gagttgcgca tccccaaccg cctcgcccaa ctcgcccgtc gcgcgcacat
     8521 cgccttccag ttcgtgcgct gccagataga ctccgttcaa gcgtcgctcg ttgatccccg
     8581 cgacgacctc cgcgttacgc gaccacaatc tcacggcatg ccccgcgcgc ccggccacca
     8641 tccccaacgc cgtgccccaa ccgcccgcac cgatgatcgc gatttgagac atcattcaaa
     8701 cgatgaagga gaaacgagga acgatgaatt gcaagcatat agcagttgtc tttcgttcca
     8761 cgctcctcgt tcctccttca tcctttccac ctgttctccg taccattcag cagcctgccg
     8821 atgttggcgc ggtgcatgaa gatgatgagc gcgccgccga tgatggcgac cgccatcagg
     8881 ggagcaaacg cggcgtcgcc gtctgactta gccctctcgc tcaagagcca gacgaagaga
     8941 ggcagcgtgg ccgtcgccat gatcgaaccg agcgagatgt agcgcgttgc ccacacgacg
     9001 gcgataaaaa ccgggacggc gagcgcgacc gctgccggcg tcaaactcaa aaagactcca
     9061 agcccggtgg cgacgccctt gccgccgcga aatctcaacc agaccggaaa gacgtgaccg
     9121 acgacgaccg ctatcgaagc ggcggccacc cactcgtcga tgccgaagtc cggcgcaagc
     9181 aaccagcgcg cggtgagcgc gacgagtgcg cctttggcgg catccagcgc gagcgtcagg
     9241 atgcctgcgc tacgtcctgc gcgccgcgtc acgttcgtcg cgcccgtgcc gccagacccc
     9301 gtctcacgca cgtcgccgcc gccacgcgcg cgcacgatca aatatccgaa cggaatcgat
     9361 cccgtcaaat acgcggcgac aataaacagc gcaggcagca atcctacctc accccgcgac
     9421 ggcgcaaaat tttaggcgac gagcaaacgg cgactataac aaccagccgg aagcggcggc
     9481 aagtgtgcgg gcgtgaggag acgcgcgccg gagttcgaat caattcagat gagacggcga
     9541 cgcaagagat cgagcgcggc ttgcgaggcg cgccagcgta tgagttggcg gtcgccgggc
     9601 agcaatagct tgcgatgctc cgtgtgcgcg tcgtccgaga gcgcgagata gacgaggccg
     9661 acgggtttgt cttccgtgcc gccgccgggt cccgcaatcc ctgtgacgct caggccgaag
     9721 tccgtccccg cgcgctcgcg cacgccctcg gccatcgctt cggccacggg
//
LOCUS       DASWNS010000392        11259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_274522, whole genome shotgun sequence.
ACCESSION   DASWNS010000392 DASWNS010000000
VERSION     DASWNS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274522"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            565..6210
                     /locus_tag="VGB76_13680"
     CDS             565..6210
                     /locus_tag="VGB76_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY0099994.1"
                     /translation="MNTRRIFLATALGLLVLAVIMPGLFGSAAEAVRQDWINAPRSGG
                     VRPPEKDVRGGVHGVPKSPVARALSETQTKGLRALQAEVGGAPLVVQYNALTSTPRHL
                     FSRAGYLTPPKEGDAENIARGFLKRWQGIFRFTDADLDALKIKSRSRLADMGMDVLVF
                     EQQVGGVPVYKGEVLVNVNREGRVLSVGSDSFPQLKVTNRVAITPAAAIEYAARDMGL
                     SGYNGQSLGATRVLAAYGSPKPRFIEGQKFSGGNLFTDEIVVTRTVFPLGAEARHAYK
                     FTLTTPQFSGIVWENVVDAQTGEVLHRFSLTMFQGQVKKERVKGVDRTASPSFGPPGG
                     GAIDSRRSSFRPDVQDLVESFNPAGTAQAKIFDSAPTGLSGRRGFGRAATRGTRPGYA
                     PENPAPASVARNSGRGFRESLVRARIENPYSVPGGALAALIYNTPFGQVLRGMPDALN
                     PSPASPFGWYYLPTGTGGAEITEDDTGRASTKNYGYTMADEAKRRNTPENAPVPCTGT
                     LPTRDCDQPFSATLTSLVGAPRVLPDGRSLSSVFESNYTEGNNVLVSDDRANDDETTN
                     GIRGYDPNRQFIATRFDFTNSYEFGNADAAGQGPGGAECLPLVGPCDVVFPASADPDI
                     FPGTLTLFYYNNILHDYMYNLGFTEATFNFQQDNFGRGGAGRDGVVAQVQDGSGTDNA
                     NMSPTVEGAKPRMQMYVFTETGFRRTDGSFDFDIVGHELMHAINNRAVGKGDTGCVGN
                     GLAGESGGQGEGWGDYLACSMADDDVEGEYATGEYDIGIRRIPYTNYRWSYNSLNGQG
                     LTRRDRSYNPAAVPDPNPGAIPFEVHDGGEYWAATLWDMRELLIMKQKVDNAYPGIFF
                     DGTRRLGSGATFYIGDRQVQSVDTKHPIEYRPEFNTHVQGLDPTVPPDPNIIAAEHIV
                     RPGAVANEIQTTGNRNGALSTALRRGAFLADLLMMRGMQLSPCNPTFVDSRDSILLAD
                     RERFGGENHAIIWRAFASHGVGALATSSSNGGVGGTVVEDFSVPAGVTTCEQQGPLTA
                     PVFVLSNNVNNTATITISPLVGAARFIISRAESADGPFVTIADVPADPINPTVYNDND
                     GGQGLILNKTYYYQVRAARDAEMNCVSGALTQSIKITVGIVVKPAPIFTGVNEVFDPK
                     ACNVLVVGWGPATSANLTADIVYDVYRSETASDPGPTTENPTNPSDAPLEPTFEPTAQ
                     NRIATGVRGTSYVDTGLKRNRVYYYIVQARDVDAGKIDTNNTGNRRVKFNAPSSPGIT
                     NTPVFAFEDFEEGEVGQDDSNLRFTPPLIDAGNDPKAEIANFQRVTGVQVSTGSETGV
                     VTSMMYGPDFNPGDPANNCGGEGGGQSDFSTTIGPITLSPDSVMEFDHFFITEAAFDG
                     GVLEVKVGGDATFNSTPYPDNITTFDLGNFIVEGGYNGRLNGDLPEVPLDGSQLQGRR
                     AYTGAKAALHHVRIPLEAFAAGGVHNPQGLPVRIRFRMVSDVLSVPSCNAGWFIDNLA
                     INNLDPASCPTLGAIGVGDLIISEFRFRGANGENDEFIELYNTTNSPIIVNAFDGSAG
                     FTLAAPDANGVLTVLTTIPSGATIPARGHYLIANNSPGGYSLKDYGGTDRALPDATYT
                     TDIPDGSGVALFRTTTPANLTLENRLDAAGHSIAPPLYREGAGIGGRPTSNGEYSHVR
                     KLTTGFPQDTNDNENDFFFIGTAGETIYGLDSDASILGAPGPENRFSPIQRNAVVKAS
                     LIDPGCSGFGTPTSACARVRTAEGANNQNAAFGTLLIRRKFKNTTNEGIRQLRFRAVN
                     ISTLGNQLPGEADIRMLSSSDLTATDSNGNDVFIEGLTLEENPPAQPGGGGLNSTVRL
                     GTITMGHPLAPGNSVNVQFRLGVMTGGNFRFLVNVEALP"
     gene            6483..10403
                     /gene="smc"
                     /locus_tag="VGB76_13685"
     CDS             6483..10403
                     /gene="smc"
                     /locus_tag="VGB76_13685"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY0099995.1"
                     /translation="MFRLQRLEITGFKSFADYTDLVFTGEGITGVVGPNGCGKSNVAD
                     AIAWVLGEQRVKHLRGAEMKDVIFQGSRNRQPSGMAEVVLHLVRDEASYEESDIDDID
                     STLEEIDERSELVEEKLLPEVAVAAEMPAADAEAAVNVEVMTVSGAVSDEVNANGNET
                     ALAVVAAGEQSPEALTAKVQQHHKRHWRPRRLALGFAPGETVTVTRRLYRDGESEYLL
                     NGRTCRLRDIQDLFSGTGLAGAHYAIIEQGRIGQILSAKPMDRRSLIEEAAGITKFRV
                     RQRAAEARLEGARGNLRRVSDIVSEIERQVGSLRRQAAKARRYRALREELRELLRHVY
                     AADERALTVALEELRTHLAASAEEVSTLASQLEAHEEESRRATAEARECEEELAAARA
                     SVADAALQRDRRERERAYQREQADALERRVSDITGQMEMVRERLATVESDRETLRAGD
                     AQLRVEAETTAQALQEAEAIYARRLAEVLEAETRIEAARAELLTHTAVAERLSEINRQ
                     LDATLERLALQAEGLEREGERARAAHAEFTSQSETLESEIKAARARLDVLNVERGAAS
                     EGVAAARSLVSATLTEYARMREEAARVRNRLDTLAELDEKRALYSQAVQRIFSAEHAD
                     EAEIDPEKDFHTIGTLADMLQVAPQWEHAVESIFGSYLQTVIVPTPDDAFRAAAWLEA
                     NNAGRATFLVAGLHGASAADAGGDYLSRGTLTDANERLTGLRLGDLLGAPREIATVLS
                     RTLPREMSARLADDLEGAMTLSFTTGDMCVTPRGEWVAGGQLLGAGNPHAVEEGTGLL
                     AFKREMRELESRAADLASEVISAEDSVSVARARLVELEDALVLIAEQIGREEREQVAR
                     ELNAAQLTQEIERAARHLRVVADDTARLAEERRELQARRTLGTAEAEAAEAARLAATV
                     SVAEAAELLAAERRAAEVEGEQLNRQRMASAAAAERRRATASELRRMETENTDLEARL
                     ERYATEIEEMRARLEELRRAMSEMDATAGTVESERAAREAEVATAAERLAEARARADA
                     LSDELTELNHRAAAARDVRATLEVNRAETAARLNYLHEACMAELAQSLEEIVTAYEAD
                     AEFDLEAGRARVEELRARVESFGAVNMMALEELAESEERLAFLTTQRQDIVDGIASTE
                     EALREIKRRSRERFRQAFEEINRNFGQLFLELFGGGRGEMSMIDADDVLESGIDIIAQ
                     PPGKRLQNVLLLSGGEKAMAALSLVLAIFRYRPSPFCLLDEVDAPLDEANIGRFTAKI
                     AEMATSTQFIVITHNKRTMEIARALYGVTMEEVGVSKLVSVKFD"
     gene            10407..>11259
                     /locus_tag="VGB76_13690"
     CDS             10407..>11259
                     /locus_tag="VGB76_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099996.1"
                     /translation="MKRENLLKAALVVAALLFQSAAIGTAAAATQSKKATRRKASRTT
                     GSKTAPEETTPDPSAARAPDAKTNNREATPETQSTPAKKATAAVAVPGAAASNALRYS
                     YEFKQPEFNVSRILIEHDGEGRGQVSFARKESAEIFTDPLEISPAALARIKTAWEALN
                     FLDSTASYQADKQFPHLGTMTLRMKQGTRERAAEFNWTHDEGVTTLVKEYRAIGEQQL
                     FIFDVTLARQYQPSDTIKIFQRLESLIKRNELSDTAQLVPLLRDLTTDERIPLMARNH
                     VTRLLKKI"
BASE COUNT         2224 a         3670 c         3557 g         1808 t
ORIGIN      
        1 attgacgaca aaacaattgc agtgttaatg tccggcactg taaacagata aaaattaaca
       61 gttcgttttg cgaggccaaa cccccccggc cttagttccg caatcccgac cgcgaactgt
      121 cgtcagaatt tccccgacga aattccagtt tgatccgtta gttgccgttc cgtcatgccg
      181 aaattttcgc tttagaaaat cttcatcttc cgtcgtatgc gccgggaagc gcagcaagtg
      241 aaggccgctc tgtcggctaa ggtcgcaaaa aactgtgggt caaggtcgag cgcaacgcgt
      301 ccgcaatgcg aatgtcgttg aaatagagct tgacgcgccg tgaaagcggc acaggctcaa
      361 gtttttctca agggcgcggg cgtaagctgt cagacatcaa ctgcggaacg ggtttctaac
      421 tcattaccta tcactcgctg ttcccgaagc tccgacaggt agtggagagg gaaagctaca
      481 ggggcggcgc atcgtgctta tcgtttgagc attcctcgat gcgacgacaa atcaaggttg
      541 ccgatgctct ggaggaacac attaatgaac acgcgtcgaa tttttctcgc gacagctttg
      601 gggctgctcg tccttgcagt catcatgccg gggctgttcg ggtctgccgc cgaagccgtg
      661 cggcaggact ggattaacgc cccgcgcagc ggcggcgtgc gcccgcccga aaaggatgtg
      721 cgcggcggcg tgcacggcgt cccgaaaagc cccgtcgcgc gtgcgctctc cgagacgcag
      781 acgaaaggtt tgagagcctt gcaggccgaa gtcggcggcg cgccgctcgt cgtgcagtac
      841 aacgctctga cgagcacgcc gcgccacctc ttcagtcgcg cgggctacct gacgccgccg
      901 aaggagggcg acgcagagaa catcgcgcgc ggcttcctca aacgctggca gggaatcttc
      961 cgcttcaccg acgccgatct cgacgcgctc aagatcaaga gccgctcgcg cctcgcagac
     1021 atgggcatgg acgtgctcgt cttcgagcag caagtgggcg gcgtgcccgt ctacaagggc
     1081 gaggtgctgg ttaacgtcaa ccgcgagggg cgcgtgctga gcgtcggcag cgacagcttc
     1141 ccgcagttga aggtgacgaa ccgcgtggcg atcaccccgg cggcggccat cgaatacgcc
     1201 gcgcgagaca tgggactctc cggctataac ggccaatcgc tcggcgcgac gcgtgtgctg
     1261 gccgcttacg gcagcccgaa accgcgtttc atcgaagggc agaaattcag cggcggcaac
     1321 ctgttcacgg acgagatcgt cgtgacgcgc accgtcttcc cgctcggcgc ggaggcgcgc
     1381 cacgcctaca agtttacgct caccacgccg cagttctccg gcatcgtctg ggagaacgtg
     1441 gtggacgcgc agacgggcga ggtcttgcac cgtttcagcc tcacgatgtt tcagggacag
     1501 gtgaagaaag agcgtgtcaa gggagtagac cgcacggcgt cgccttcgtt cggcccgccg
     1561 ggaggcggcg cgattgacag tcgccgctcg tcgttccgcc cggacgtgca agacctcgtt
     1621 gagagcttca acccggcggg cacggctcaa gcgaaaatct tcgactccgc gcccaccggc
     1681 ctgagcgggc gacgcggctt cggtcgcgcg gcgacgcgcg gcacgcgccc cggctacgcg
     1741 ccggaaaacc ccgcccccgc cagcgtcgcg cgcaacagcg gtcgcggctt ccgcgaatcg
     1801 ctcgtccgtg cgcgcatcga gaacccgtat tcagttcccg gcggcgcgct cgccgcgctc
     1861 atctacaaca cgcctttcgg tcaggtcttg cgcggcatgc ccgacgcgct caacccttcg
     1921 cccgcttcgc ccttcggctg gtactacctg cccaccggca cgggcggcgc ggagatcacc
     1981 gaagacgaca ccggacgcgc ctccaccaag aactacggct acacgatggc tgacgaggcc
     2041 aagcggcgca acactcccga aaacgcgccc gtaccctgta ccggcacgct gccgacgcgc
     2101 gattgcgacc agcctttctc cgctaccctg acgtcgctcg tcggcgcacc gagagtgttg
     2161 ccggacggtc gctcgctctc gtccgtcttc gagtcgaact acacggaggg caataacgtc
     2221 ctcgtctccg acgaccgcgc caacgacgac gagacgacca acggcattcg cggctacgac
     2281 ccgaaccgcc agttcatcgc cacgcgcttc gacttcacga acagctacga attcggcaac
     2341 gcagacgcgg caggacaagg ccccggcggc gcggaatgtc tcccgctcgt cggcccctgc
     2401 gacgtggtct tccccgcgag cgcagacccg gacatcttcc ccggcacgct cacgctcttc
     2461 tactacaaca acatcctgca cgattacatg tacaacctcg gcttcaccga agccacgttc
     2521 aacttccagc aggacaactt cggacgcggc ggcgcggggc gcgacggcgt cgtcgcacag
     2581 gtgcaggacg gttccggcac tgacaacgcc aatatgtcgc ccacggtcga aggcgcgaag
     2641 ccgcgcatgc agatgtacgt ctttacagag acgggcttcc gccgcaccga cggctctttc
     2701 gacttcgaca tcgtcgggca cgaactcatg cacgccatca acaaccgcgc cgtcggcaaa
     2761 ggcgacacgg gctgcgtggg caacgggctg gccggagagt ccggcgggca gggcgaaggc
     2821 tggggcgact acctcgcctg ctcaatggca gacgacgacg tggaaggcga atacgccacg
     2881 ggcgaatacg acatcggcat ccgccgcatc ccttacacca actatcgctg gtcttacaac
     2941 tcgctcaacg gtcagggact cacacgccgc gaccgcagct acaacccggc agccgtgccc
     3001 gacccgaacc ccggcgcgat cccgttcgag gtgcacgacg gcggcgagta ctgggcggcc
     3061 acgctctggg acatgcgcga gttgctcatc atgaagcaga aggtggacaa cgcttatccc
     3121 ggcatcttct tcgacggcac gcgccgtctc ggctccggcg caactttcta tatcggcgac
     3181 cgtcaggtgc aatccgttga cacgaagcac ccgattgaat accgccccga gttcaacacg
     3241 cacgtgcagg gactcgaccc gaccgtgccg cccgacccga acatcatcgc cgccgagcac
     3301 atcgtccgcc ccggcgcggt cgccaacgag attcaaacga ccggcaaccg caacggcgcg
     3361 ctctcgaccg ctctccggcg cggcgcgttc ctcgccgacc tcctgatgat gcgtggcatg
     3421 cagctctcgc cctgcaaccc gaccttcgtt gattcgcgcg actcgatcct gctcgccgac
     3481 cgcgaacgct tcggcggcga aaatcacgcc atcatctggc gcgcgttcgc cagccacggc
     3541 gtcggcgcgc tcgccacctc ttcgtcgaac ggtggcgtcg gcggcacggt cgtcgaagac
     3601 ttctccgtcc cggcgggcgt cacgacctgc gaacagcagg ggccgcttac cgctccggtc
     3661 ttcgtcctct ccaacaacgt caacaacacg gccaccatca ccatttcgcc cctcgtgggc
     3721 gcggcgcgct tcatcatctc gcgcgccgag agcgcggatg gcccgttcgt caccatcgca
     3781 gacgtgcccg ccgacccgat caacccgacc gtctataacg acaacgacgg cgggcaaggg
     3841 ctgattttga acaagaccta ctactatcag gtgcgcgccg cgcgcgacgc cgagatgaac
     3901 tgcgtctctg gcgcgttgac gcaaagcatc aagatcactg tcggcatcgt cgtcaaaccc
     3961 gcgcccatct tcacgggcgt caacgaagtc ttcgacccga aggcttgcaa cgtgctcgtc
     4021 gtcggctggg gtcccgcgac ctccgccaac ctgaccgccg acatcgtcta cgatgtctac
     4081 cgttcggaga cggccagcga tccgggacca accaccgaaa acccgaccaa tccttccgac
     4141 gcgccgctcg aaccgacctt cgagccgacg gcgcagaacc gcatcgccac gggcgtccgt
     4201 ggcacgtcct acgtggatac gggactcaag cgcaatcgcg tctattacta catcgtgcag
     4261 gcgagagacg tggacgccgg taagatcgac acgaacaaca cgggcaaccg cagggtcaag
     4321 ttcaacgcgc cgtcgtcgcc cggtatcacg aacacgccgg tcttcgcctt cgaggacttc
     4381 gaggagggcg aggtcgggca ggacgactcg aacctgcgct tcacgccgcc gctcatagac
     4441 gcgggcaacg atccgaaggc agagattgcg aacttccagc gcgtcacggg cgtgcaagtc
     4501 tcgaccggct cggaaacggg cgtcgtgacg agcatgatgt acgggccgga cttcaacccc
     4561 ggcgatcccg ccaacaactg tggcggcgaa ggcggcgggc aaagcgattt ctccaccacc
     4621 atcggcccca tcacgctcag tcccgactcc gtgatggagt tcgaccactt cttcatcacc
     4681 gaggcggcct tcgatggcgg cgtgttagag gtcaaagtcg gcggcgacgc gaccttcaac
     4741 tcgacgccct acccggacaa catcaccact ttcgacctcg gcaacttcat cgtcgaaggc
     4801 ggttacaacg ggcgtctcaa cggcgacctg ccggaagttc cgctcgacgg ttcgcaactg
     4861 caaggacggc gcgcttacac gggcgcgaag gccgcgctgc accacgtccg catcccgctc
     4921 gaagccttcg cggcgggcgg cgtgcacaac ccgcagggct tgcccgtgcg catccgcttc
     4981 cgcatggttt ccgacgtgct gagcgtcccg tcctgcaacg cgggctggtt catagacaat
     5041 ctggcgatca acaatctcga cccggccagt tgtcccacgc tgggcgcaat cggcgtcggc
     5101 gatctcatca tcagcgagtt tcgcttccgt ggcgcgaacg gtgaaaacga cgagtttatc
     5161 gagttgtaca acacgacgaa ctcgcccatc atcgtcaacg ccttcgacgg ctcggcaggc
     5221 ttcacgctcg ccgcgcccga cgcgaacggc gtgctcaccg tgctcaccac cattccgagc
     5281 ggcgcgacca ttccggcgcg cggccattac ctcatcgcca ataacagtcc ggggggctac
     5341 agcctcaagg actacggcgg cacggacaga gctttgcccg atgccaccta cacgaccgac
     5401 attccggacg gctccggcgt cgcgctcttc cgcacgacca cgcccgcgaa cctgacgctc
     5461 gaaaaccgtc tcgacgcagc cggacacagc atcgcgccgc cgctctacag agagggcgca
     5521 ggcatcggag gccgcccgac atccaacggg gagtacagtc atgtacgcaa actgacgacc
     5581 ggcttcccgc aggatacgaa cgataacgag aacgacttct tcttcatcgg cactgcgggc
     5641 gagacgatct acggtcttga tagcgatgcc tccatcctcg gcgcgccggg tcctgaaaac
     5701 aggttcagcc cgatccagcg caacgccgtc gtcaaggctt cgctcatcga ccccggttgc
     5761 tccggcttcg gcacgcccac gagcgcatgc gcacgcgtcc gcacggcaga gggagccaac
     5821 aaccagaacg cagccttcgg cacgctcctg attcgtcgca agttcaagaa cacgacgaac
     5881 gagggcatcc ggcagttgcg tttccgtgcc gtgaacatca gcacgctcgg caatcaactg
     5941 cccggcgagg ccgacatccg catgctctct tcgagcgacc tgacggcgac agactcaaac
     6001 ggcaatgatg tcttcatcga agggctgaca ctggaagaga atccgcccgc gcaaccgggt
     6061 ggcggaggtc ttaactcgac cgtgcggctc ggcacgatca cgatgggtca cccgctcgcg
     6121 ccgggcaact ccgtcaacgt gcaattccgt ctgggcgtga tgaccggcgg caatttccgg
     6181 ttcctggtca acgtcgaggc attgccgtaa aacgtgcggc actcgccgcg cattcacaaa
     6241 ctgcccgcgc cgccgaagtt tgcaaagact ccggcggcgc ggttttcttg ccgccgcgcc
     6301 tgcgcgcgac gaaatctaaa ccgccttatt tcatcaataa gggcgtttca cctgagagtg
     6361 tggttgacac gcgcccctct ccaacatata gtgtgcctct cccacgccgc gcctagcggc
     6421 tgtgctttta gacaaaacac cttgtccgct gttcgcgtct ctcgcagacg aaagttgcac
     6481 gcatgttcag attacaaaga ttagaaataa ccggcttcaa atcattcgcc gattacaccg
     6541 acctcgtctt cacgggcgaa ggcatcacgg gcgtcgtcgg tccaaacggc tgcggcaagt
     6601 cgaacgtggc cgacgctatc gcctgggttt tgggcgagca gcgcgtcaag cacctgcgcg
     6661 gcgcggagat gaaagatgtc atctttcaag gctcccgcaa tcgccagccg agcggcatgg
     6721 ccgaagtcgt gctgcatctc gtgcgcgatg aagcatcgta tgaagagtcg gacatagacg
     6781 acatagattc cacgctcgaa gagattgacg agcgcagcga actcgtcgaa gagaaactgt
     6841 tgccggaagt ggcggtggcg gcagagatgc ccgccgctga tgccgaggcc gccgtcaacg
     6901 tggaggtgat gacggtttcc ggcgcggtgt ctgatgaggt aaatgctaac gggaatgaga
     6961 cggcgttggc agtggtcgcc gccggggagc agagtccgga agcgttgacg gcgaaagttc
     7021 agcagcatca caaacgtcac tggcgtccgc gtcgtctcgc gctcggattt gcaccgggcg
     7081 agacggtgac ggtgacgcgc aggctctatc gcgacggcga gagcgagtat ctactgaacg
     7141 ggcgcacctg tcgtctgcgc gacatacaag acctgttctc aggcacgggg ctggcgggcg
     7201 cgcactacgc gatcatcgag cagggacgca tcggacagat tctttcggcg aagccgatgg
     7261 atcgccgttc gctgattgaa gaggcggcgg ggatcacgaa gtttcgcgtg cggcagcgcg
     7321 cggcggaagc gcgtctggaa ggagcgcgcg ggaacctgcg tcgcgtctcg gatatcgtct
     7381 cggagatcga gcggcaggtc gggagtttgc gccggcaggc ggcgaaggcg cgtcgttacc
     7441 gtgcgctgcg cgaagagttg cgcgaacttt tgcgccacgt ctatgcggcg gacgagcggg
     7501 ctttgaccgt cgcgctcgaa gaattgcgca cgcatctggc cgcctcagcc gaagaggtga
     7561 gcacgctcgc gtcgcaactc gaagcgcatg aagaggagtc gcggcgtgct acggcggaag
     7621 cgcgcgagtg tgaagaagaa ctcgcggcgg cgcgtgcgtc tgtggcggac gcggccttgc
     7681 agcgcgaccg gcgcgagcgc gaacgcgctt atcaacgcga gcaggccgat gctttagagc
     7741 gtcgcgtttc agacatcacg ggacagatgg agatggtgcg cgaacgtctg gcgacggttg
     7801 agtccgaccg cgagacgctg cgcgcgggcg atgcgcagtt gcgcgtcgaa gcggaaacca
     7861 ctgcacaggc gttgcaggaa gccgaagcga tctatgcgcg tcgcctcgcg gaagtgctgg
     7921 aagccgagac gcggatcgag gcggcgcgcg cagagttgct gacgcacacg gcggtcgccg
     7981 aacgtctctc ggagatcaac cgccagttgg atgcgacgct cgaacgcctc gccttgcagg
     8041 ccgaagggct ggagcgcgaa ggcgaacgcg cacgcgccgc acacgccgag ttcacctcgc
     8101 aatccgagac gctcgaatcc gagatcaagg cggcgcgcgc gcgcctcgac gtgttgaacg
     8161 tcgaacgcgg cgcggcgtcg gagggcgtgg ccgccgcgcg gagtctggtg agcgcgaccc
     8221 taacggagta tgcgcggatg cgtgaagagg ctgcgcgcgt gcgcaatcgt ctcgacacgc
     8281 tcgccgaact cgacgagaag cgggcactct attcgcaggc cgtgcagcgc atcttttccg
     8341 cagagcacgc ggacgaagcc gagatcgatc cggagaaaga ttttcacacc atcggcacgc
     8401 tcgcggacat gctgcaagtc gcgccgcagt gggagcacgc cgtcgagagc atcttcggtt
     8461 cgtacctgca aaccgtgatc gtgccgacgc cggacgatgc ttttcgcgcc gccgcgtggc
     8521 tcgaagccaa caacgccgga cgcgccacct tcctcgtcgc cgggcttcac ggcgcgagcg
     8581 cggcggatgc gggcggcgat tatctttcgc gcggcactct gaccgacgcg aacgagcgtc
     8641 tgaccggact gcggctcggc gacctgctcg gtgcgccgcg cgagatcgcg acggtgctct
     8701 cgcgcacgct cccgcgtgag atgagcgcgc gcctcgcgga cgatctggag ggcgcgatga
     8761 cgctctcctt cacgacgggc gacatgtgcg tgacgccgcg cggcgaatgg gtggcgggcg
     8821 ggcaactgct cggcgcgggc aatccgcacg cggtcgaaga gggcacgggg ctgctcgcgt
     8881 tcaagcgcga gatgcgcgaa ctcgaatcgc gcgccgcaga cctcgcttcg gaagtcatct
     8941 cggcggagga ttcggtgagc gtggcgcgtg cgcggctcgt cgagttggag gatgcgctgg
     9001 tcttgatcgc ggagcagatc gggcgcgagg agcgcgaaca ggtggcgcgc gaactcaacg
     9061 cggcgcagtt gacgcaggag atcgagcggg cggcgcgtca cctgcgcgtc gtggccgacg
     9121 acacggcgcg gctggcggaa gagcggcgcg agttgcaagc ccggcgcacg ctggggacgg
     9181 cggaagcaga agcggcggaa gcggcgcgtc tggccgccac agtctcggtc gcggaagcgg
     9241 cggaactttt ggccgccgaa cgacgcgcgg cggaagtcga aggcgagcag ttgaatcgcc
     9301 agcggatggc ctcggctgcc gccgccgaac gcaggcgcgc gacggcctcc gaactccggc
     9361 gcatggagac ggagaacaca gacctcgaag cgcgactcga acgctacgcc acggagatcg
     9421 aagagatgcg tgcgcggctc gaagagttgc ggcgcgcgat gagcgagatg gatgcgaccg
     9481 ccggaacggt tgagagcgaa cgcgccgcgc gcgaagccga agtggcgacg gcggcggagc
     9541 gtctggcgga agcgcgtgcg cgcgccgacg cgctctcaga cgagttgacg gagttgaacc
     9601 atcgtgcggc ggcggcgcgg gatgtgcgtg cgacgctcga agtcaaccgt gcggagacgg
     9661 cggctcgttt gaactatctg catgaagcct gcatggccga actcgcgcag tcgctcgaag
     9721 agatcgtgac ggcctatgag gcggacgcgg agtttgatct ggaagcggga cgcgcacggg
     9781 tcgaagagtt gcgcgcacgc gtggagagtt tcggcgcagt caacatgatg gcgctcgaag
     9841 aactggccga gtcggaagaa cggctggcct tcctgacgac gcagcgacag gacatcgttg
     9901 acggcattgc ttcgacggaa gaagccctgc gcgagatcaa gcgacgttcg cgcgagcgtt
     9961 tccgtcaagc cttcgaagag atcaatcgta atttcgggca actcttcctc gaactcttcg
    10021 gcggcgggcg cggcgagatg agcatgatcg acgcggacga cgtgttggag tcagggatcg
    10081 acatcatcgc gcagccgccg ggcaagcggc tgcaaaacgt gctgctgctt tcgggcggcg
    10141 agaaggcgat ggcggctctg tcgctcgtgc tcgccatctt ccgttatcgc ccgtcgccct
    10201 tctgcctgct cgacgaagtt gacgcgccgc tcgacgaggc caacatcgga cgcttcacgg
    10261 cgaagatcgc agagatggca accagcacgc agttcatcgt catcacacac aacaagcgca
    10321 cgatggagat cgcacgcgcg ctctacggcg tgacgatgga agaggtcggc gtctcgaaac
    10381 ttgtctcggt taagtttgat tgaaacgtga agagagagaa tttactcaag gccgctttag
    10441 tcgtcgccgc gcttcttttc cagagcgcgg cgattggcac tgcggcagcc gccacgcaga
    10501 gcaagaaagc cacccggcgg aaagcgagcc ggacgacggg cagcaaaacc gctcccgaag
    10561 agacgacgcc cgacccctcc gccgctcgcg cgcccgacgc caaaaccaac aatcgtgaag
    10621 cgacgcccga aacgcagagc acgcccgcca aaaaagcgac ggcggctgtt gctgtgcccg
    10681 gcgcggctgc gtcgaatgcg ttgcgctact cttacgaatt caaacaaccg gagtttaacg
    10741 tcagccgcat cttgatcgag cacgacgggg aggggcgcgg gcaggtttcg ttcgcgcgaa
    10801 aggagagcgc ggaaatcttc accgacccgc tcgaaatctc gcccgccgcg ctcgcgcgca
    10861 tcaagacggc atgggaagcc ctgaattttc tcgactcgac tgcgagctat caggccgaca
    10921 agcagtttcc gcatctcggt acgatgaccc tgcggatgaa gcagggcacg cgcgaacgcg
    10981 cagccgagtt caactggacg catgatgagg gagtcactac gctcgtcaag gaatatcgcg
    11041 ccatcggcga gcagcaactc ttcatcttcg acgtgacttt agcgcgacag tatcaaccgt
    11101 ccgacacgat caaaatcttc cagcgtctcg aaagcctcat caagcgcaac gagctgtccg
    11161 acactgcgca actcgtcccg ctcctgcgcg acctcacgac ggacgaacgc atcccgctca
    11221 tggcgcgcaa ccacgttacg cgcctgctta agaagatcg
//
LOCUS       DASWNS010000393        10945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_248724, whole genome shotgun sequence.
ACCESSION   DASWNS010000393 DASWNS010000000
VERSION     DASWNS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248724"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="VGB76_13695"
     CDS             complement(<1..1889)
                     /locus_tag="VGB76_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0099997.1"
                     /translation="MSTKTQPAARVIFAFLFALLCLAGGCERAPQLPDKNSKEYREAI
                     SAFYVGLAGLQVGDDVRAESDLARLVALVPAEPAGWANYGLLALRQRNLDVAAERFEK
                     ARALASDNSELHFLIGLLESTRGRFPEAVTALRKAIELDPRNLKAAYTLAEQLERQGD
                     DASALEVQQLFQKILDAQPDNLAAQLELTRVAAKRGDADTLRASVARLAANAAAWPPE
                     VQQQWSALQTAAANDPRAAATRVAFLRNVLVRVPEYRRSLAQVKSAPGEEAEPFARPL
                     KLAAPEFTPAAPDADLTFNAEGAGTGDTESWDTSVAVTLDAESQPVVINADARGLRLP
                     GGVTLAFPPASSQIAATTPDHVLALDFNYDFKTDLVLAGAGGVRLFRQDAPDKFTDVT
                     AATMLSNAIVNASYAGVWAADIEADGDLDIVTGANAGEAVVMRNNGDGTFKELRPFTG
                     ASNLRRFVWADFDADGDPDAATLDAEGRLAVFTNERVGQFRPRSIPAATTQAVALAAA
                     ETSGDEALDLLLLKSDGAILRLSDRDEGRDWTSAEIARVALRPDNEPSNDRARLFAAD
                     LDNSGALDLIISDSGGAQVLLGDAQGKFTPLKSTFAARVSSVADINGDGTFKELRPFT
                     GASNLR"
     gene            2046..5681
                     /locus_tag="VGB76_13700"
     CDS             2046..5681
                     /locus_tag="VGB76_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0099998.1"
                     /translation="MKTLKRFILSLAILGGALILTYTGSTRSASPARAQAPSSSTAAS
                     REEAYRANNLGVAMLEQFKHKEGAEQFARALKIDPMLAVARANLSIALYNVPDLAGAQ
                     REAQAAAALNPNAPQPFYVLGMIAKTQNRADEAVAAFQRVLKIDPGDVGANVNLGQLY
                     AQQLKYAEAIAVFRTALLTEPYNTTALYNLATSLIRGGDRAEGQKLIQLFQEIRRRGS
                     GTTIGTTYLEQGRYAEATASTGLEPDLVDKTTPAAGFTDATAATISPAPAADTGAGAP
                     VRSIIGRRFKASEFGDNARRDIAAALGQSVLLFDYDADNDLDLLQASGSELRLYRNDG
                     GKLTDATAQSGALAAKTSGAWTASVAGDYDNDGRPDLFLMRDTGGALFHNDGGGKFSD
                     ATRTVGLPSYPYLSASVAFLDADHDGDLDIFVAGLADLSKPLAGKDTFIFPEDFNAAP
                     GMLWRNDGNGKFTDVTTDAKLGAPSRAFAVVPTDFNNKRDVDLLVVNYNAAPDLYSNQ
                     RDGSFKNVTRDVGLDVSGRWTSAAAGDYNKDGFTDFFFGRADGAGLFATSDGRGRFTT
                     NPAPDGTERTTAAQFLDYDGDGLLDCATFADGSLKVWRNVGDGWANVSERAVARDLSG
                     KAAAESNELVRHTFASGDIEADGDVDLFAVAPAGALRVALGGGGVNRSLRVNLAGRVS
                     NRSAIGAKVEVRAGSLVQKLETYSASPAPAPADIQVGLGKRQTADAVRVLWPAGIVQA
                     ETEIAAPPTGAGKNASASLSITELDRKPSSCPYLYAWNGERFAFVTDFMGGGEIGYWV
                     GAGGRYNTPDPVEYVRIRGDGLKPRAGRYELRISNELEEAVLFDRLQLVAVAHLEGTE
                     VYPNEGMTSAPLPPFKLYTTKGARPPLAAYDERGDVLARVAALDREFVEGFPLDAIRG
                     YAAKHSLTLKLAEESAMQSRLLLLLTGWTDYAFSSDNVAAAQAGKTLRPPALQVKNAR
                     GEWQTVIENIGIPVGRPQTVPVDLTGKFLTTNREVRIVTNMRIYWDQVLVDTSSTHAP
                     VRMTRLDPAVADLRWRGYSAEETPDGREPFTYAYERVSYTSPWKVMPGRYTREGDVRK
                     LLRTTDDMFVITRPGDEIILSFDANKLPPLPAGWTRTFLLYADGFSKEMDINSATPDG
                     LEPLPFHGMTRYPYSAPEAYPLTPARRAYLERYNTRIVRGSLPQIETVFAEDMIFNAT
                     RSLEGEK"
     gene            6030..6176
                     /locus_tag="VGB76_13705"
     CDS             6030..6176
                     /locus_tag="VGB76_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0099999.1"
                     /translation="MTEPLVGDIAEDLSKVSKGLGALKEVWSFSSSVAALSGAVQVQG
                     HPHR"
     gene            6190..6624
                     /locus_tag="VGB76_13710"
     CDS             6190..6624
                     /locus_tag="VGB76_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100000.1"
                     /translation="MFGFGEKPNPLAFVEKHLVEINAKLDRVLVGIDEIKVGMLGQAI
                     LAAYLDMSDPVYLIKKYFEDLASYMEAGHTFTDAERKEFVEKVLGTRSERDGVAFCAH
                     KIVKLAPTQSPLFFELLFPYLAAAATPGNAREMRVATLRSGG"
     gene            complement(6840..7697)
                     /locus_tag="VGB76_13715"
     CDS             complement(6840..7697)
                     /locus_tag="VGB76_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100001.1"
                     /translation="MASEVKKTIVDTATSDLTTRAIALLLPLLLSLVSALVPPVRDRI
                     LPVLPRPLLAVLIGISLSANLALFFYVLRLRKTQRQLIEQRDTAKTFIKEFTVKWDEH
                     LEPHCPVCLAELAPVIHPVSGIADIGFFKCIPCKHGYRLTNEATGKVLTLLEAKALLS
                     SNEPPRVIAAPSFDRTKLRQAKSDLESLKANLPNSDVIETSIISYHGILDRLKSETDY
                     DLTNFRISPSDIKRKERKYPATRSVSRPFNNKPAYSTWSEEGYCSKQIFHTAIDSAIN
                     RLKLELEGG"
     gene            7995..9263
                     /locus_tag="VGB76_13720"
     CDS             7995..9263
                     /locus_tag="VGB76_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0100002.1"
                     /translation="MDARQQKGQEIADKSNITRKGHLWLVPSQNGSGAYTVNLESDKP
                     HCTCRDHEFRRATCKHIVAVQVTIERTKTVTTTTLEATDGQAAITETVTTETVKVKRT
                     TYRQEWKQYNAAQAYEKSEFLALLYGLTNGVDEPVQTFGRPRLSMADTLFAVVYKIYS
                     TVSTRRFASDLRDAFQKGYLSKLPSYNTIFDYLQMPELTPYLQSLITESSKALKAVET
                     DFAVDSSGFSTGQFMRWTDVKYGQKEDARQWLKLHLMCGVRTNVVTSVEVGGGYSSDY
                     NYFKPLVDRTAQSGFTMKEVSADKAYLGTTNFAATLKHGAIPYIPFKSNSQPNGNGAL
                     WARIYHFYNFQREEFLQHYHKRSNVETTFSMIKAKFGQRLRTKTPIAQTNEALCKVLC
                     HNICCVIQSIHELGIETTFNAESEVARKVG"
     gene            complement(9542..10054)
                     /locus_tag="VGB76_13725"
     CDS             complement(9542..10054)
                     /locus_tag="VGB76_13725"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY0100003.1"
                     /translation="MEEIRQVINAALGLGIENKDINVWQMCLRAVIVFILAIGMLRVG
                     DKRFMGRSTAFDVMLGIIFGSVVSRAITGNAPFLPTLAASLVLVLIHWALAAIAFRAH
                     GFSTMVKGHDRLLVCDGEVQPDAMRKSHITEHDLREALRINGQTPDLSRVQEARLERN
                     GDISTISKKD"
     gene            complement(10226..>10945)
                     /locus_tag="VGB76_13730"
     CDS             complement(10226..>10945)
                     /locus_tag="VGB76_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100004.1"
                     /translation="EPGTYSITIRQPLSIVSKESPLEVTRPVVVPTVDILEDLWQFSH
                     LTFSFVVRFFIGALLLSYGMIQSNLLLIIAGLLFMPFLPLLLAVGFGLWTREWRLAGQ
                     GILALLVATGLTATGSIVVALMTGPPMRFEPSSSLSATFLISLAIGVAASLATADDVG
                     RREMIGLAATSQVALIPAWLGISSVFGFSPLAVTSPVESLATFFLVIGTVVGAALITY
                     ALLGMRGAAVRHFISRAIRKN"
BASE COUNT         2345 a         3178 c         3194 g         2228 t
ORIGIN      
        1 cgtagattgc tcgcgcctgt gaacgggcgc aactctttga acgtgccgtc gccgttgatg
       61 tcggcgacgg aagagacgcg cgccgcgaac gtagatttga gcggcgtgaa tttgccctgc
      121 gcgtcgccga gcaaaacttg cgcgccacct gaatcggaga tgatgagatc gagcgcgccg
      181 ctgttgtcca gatcagccgc gaacaggcgc gcacggtcgt tcgacggttc gttgtccggc
      241 ctgagagcga cgcgtgcgat ttccgcgctc gtccaatcgc gcccttcgtc tctgtcggag
      301 aggcggagaa ttgcgccgtc gcttttgagc aggaggagat cgagagcctc gtcaccgctg
      361 gtttccgccg ctgcgagcgc gacggcctga gttgtcgcgg cagggatgct gcgcggtctg
      421 aactggccga cgcgttcgtt tgtgaaaacc gcgagacgac cctcggcgtc gagagtcgcg
      481 gcgtcggggt cgccgtcggc gtcgaagtca gcccagacga agcggcgtag attgctcgcg
      541 cctgtgaacg ggcgcaactc tttgaacgtg ccgtcgccgt tgtttctcat gaccacggct
      601 tcgcccgcgt ttgctccggt gacaatatcg aggtcgccgt cggcttcaat gtccgccgcc
      661 cacacgcccg cgtaagaagc gttgacgata gcgttggaga gcatggtcgc ggcggtgaca
      721 tcggtgaatt tgtcgggcgc gtcctgtcga aagagacgca cgccgcccgc gcccgcgagg
      781 actaaatcgg tcttgaagtc gtagttgaaa tctagagcga gaacgtggtc gggggttgtc
      841 gcggcaattt gagacgaagc gggagggaag gcgagcgtga cgccgccggg cagacgcagg
      901 ccgcgcgcgt cggcgttgat gacgacgggc tgactctccg cgtcgagcgt gacggcgacg
      961 ctcgtgtccc aactttcagt gtcgcccgtg cccgcgcctt cggcgttaaa agttaaatcg
     1021 gcgtcaggcg cggcgggtgt aaattccggc gcagcgagtt tgagcgggcg cgcgaaaggc
     1081 tccgcctctt cgccgggcgc ggatttgacc tgcgccagac tgcgtcgata ttcaggcacg
     1141 cgcacgagca cgttgcgcag gaatgcgacg cgcgtggcgg cggcgcgcgg atcgtttgcg
     1201 gcagccgtct ggagcgcgct ccattgctgt tgaacttcgg gcggccacgc ggcggcgttc
     1261 gcggcgagac gcgcgacgga tgctctcaac gtgtcggcgt cgcctcgttt cgcggcaacg
     1321 cgcgtcagtt ccagttgcgc ggcgagattg tccggctgtg cgtctaaaat tttctgaaaa
     1381 agctgttgga cttctagcgc gcttgcgtcg tcgccctgcc gttcgagttg ctcggcgagc
     1441 gtgtaggcag ccttgagatt gcgcgggtcc agttctatcg ctttgcgtag cgccgtgacg
     1501 gcttcgggga aacgcccgcg cgtgctttca agcaggccga tcaggaaatg aagctcgctg
     1561 ttgtcggagg cgagtgcgcg cgctttctcg aaacgctcgg cggccacgtc cagattgcgc
     1621 tggcgcaagg cgagcagccc gtaattagcc cagcccgcag gctccgccgg gaccagtgcg
     1681 acgagccgcg cgaggtcgct ttcggcgcgc acgtcgtccc cgacctgcaa cccagccaac
     1741 ccgacgtaga aggcggaaat cgcctctcga tattctttgg aatttttatc cggcagttgc
     1801 ggcgcgcgct cgcagccgcc cgcgaggcac aggagcgcga agagaaaagc gaagataacc
     1861 cgtgcggcgg gctgcgtttt agttgacatt aggattttat gacgcggcaa cgtcacacgt
     1921 ttcgttgtat aacgaggcac gcggcgtggt ttcacgtttt aaccgcttgc gagcttatat
     1981 gctactttac cagatgccaa aatgatctgc atcagggctg ccttttgttg tagaagcaag
     2041 caactatgaa gacgctcaaa cggtttattc tctcgctcgc catcctcggc ggcgcgttga
     2101 ttttgacgta cacaggttcg acccgcagcg catcgcccgc acgcgctcaa gctccctcgt
     2161 cgtctacggc ggcgagccgg gaggaagcct atcgcgcgaa caatctgggc gtggcgatgc
     2221 ttgaacagtt caagcacaag gaaggtgcgg aacagttcgc gcgcgcgttg aagattgatc
     2281 ccatgctcgc ggtcgcgcgc gcaaacctga gcatcgcgct ctacaacgtg cccgatctcg
     2341 cgggcgcgca gcgcgaggcg caggcagccg ccgcgctcaa cccgaacgcg ccgcagcctt
     2401 tctatgtgct cggcatgatc gcgaagacgc agaaccgcgc ggacgaagcc gtggcggcgt
     2461 tccagcgtgt tttgaagata gacccgggcg atgtcggcgc gaatgtgaat ctcggtcaac
     2521 tctacgcgca gcaactcaag tacgctgaag ccatcgccgt ctttcgcacc gcgctcctga
     2581 cggagcctta taacacgacc gctctctaca atctcgccac gtcattgatt cgcggcggcg
     2641 accgcgccga aggtcagaaa ctgattcaac tctttcagga gatacgccgt cgcggaagcg
     2701 ggacgacaat tggcacgact tatctggaac agggacgcta cgcagaagcc accgcctcca
     2761 ccggcctcga acccgacctc gtcgataaga cgacgccagc cgccggtttc acggatgcca
     2821 ccgctgccac catctcgccc gctccggcgg cggacacggg agcgggcgca ccagtccgct
     2881 caatcatcgg gcgtcgtttc aaggcgagcg agttcggcga caacgcaagg cgcgacatcg
     2941 cagccgcgct cggccagagc gtcttgcttt tcgattacga cgcggataac gatctcgacc
     3001 tgctacaggc gagcggcagc gaactgcgtc tctatcgcaa cgacggcggc aagctaacgg
     3061 atgcgacggc gcagagcggc gcgctcgccg cgaagacgag cggcgcatgg acggcgtcgg
     3121 tcgcaggcga ttacgacaac gacggcagac ccgacctgtt cctcatgcgc gacacaggcg
     3181 gcgcgttatt ccataacgac ggcggcggaa agttttccga tgcgacgcgc accgtcggcc
     3241 tcccgtctta tccatatctc tccgcctccg tcgccttcct cgacgccgac cacgacggcg
     3301 atctggacat cttcgtcgcc ggactcgccg acctgtcgaa gccactggcg ggcaaagata
     3361 cgttcatctt tcccgaagac ttcaacgccg cgccgggcat gctctggcgc aacgacggga
     3421 acggtaaatt caccgatgtc acgaccgacg cgaagctcgg cgcaccctct cgcgccttcg
     3481 ccgtcgtccc cacggacttc aataacaagc gcgacgttga tctgctggtc gtcaactaca
     3541 acgccgcgcc cgatctctac agcaaccagc gcgacggctc tttcaagaac gtcacacgcg
     3601 atgtcggcct cgacgtgagc gggcgttgga cttccgctgc ggcgggcgac tacaacaaag
     3661 acggtttcac ggacttcttc ttcggtcgcg ccgacggcgc aggtctgttt gccacgagcg
     3721 acgggcgagg ccgcttcacg accaaccctg cgccggacgg gacggagcgc acgaccgccg
     3781 cgcagtttct cgattatgac ggcgacggtt tgctcgattg tgcgacgttc gcggatggct
     3841 cgttgaaggt ctggcgcaac gtcggagatg gctgggctaa cgtctcggag cgcgccgtcg
     3901 ccagagattt atccggcaag gcagcggcag agtctaacga attggtgcgg cacacctttg
     3961 cgagcggcga catcgaagcg gacggagacg ttgatctgtt tgccgtcgcg cctgcgggag
     4021 cattgcgcgt tgcgctcggc ggtggtggcg taaaccgctc gttgagggtc aatctcgcag
     4081 ggcgcgtcag taaccggagc gcaatcggag cgaaggtgga ggtgcgcgcc ggaagtctgg
     4141 ttcagaaact cgaaacctat tcggcgtcgc ccgcgcctgc gcccgcggac attcaagtgg
     4201 gtctgggcaa acgccagacg gcagatgcgg tgcgcgtgct ctggcctgcg ggaatcgtcc
     4261 aagcggagac ggagattgcc gccccgccga ccggcgcggg gaagaacgct tcggccagtc
     4321 tctcgatcac cgaactcgac cgcaagcctt cgtcgtgtcc ttacctctac gcctggaacg
     4381 gcgagcgttt tgcgttcgtc acagatttta tgggcggcgg cgagataggc tactgggtag
     4441 gcgcgggcgg gcgttacaac acgcccgacc cggtcgagta tgtgcgcatt cgcggcgacg
     4501 ggttgaagcc gcgcgcaggg cgttacgagc tgcgcatctc gaacgagttg gaagaggctg
     4561 tcctctttga tcgtttgcaa ctcgtggcgg tcgcgcacct ggaaggcacg gaagtttacc
     4621 cgaacgaagg catgacgagt gcgcctctac cgccgttcaa actctacacg acgaagggcg
     4681 cgcgcccgcc tctcgcagcc tatgacgagc gcggcgacgt gctcgcgcgc gtggccgcct
     4741 tagatcgcga atttgtcgaa ggcttcccgc tcgatgcgat acgcggttac gccgccaaac
     4801 attccctgac gttgaaacta gctgaagagt cggcgatgca aagtcgcctg ctgctgcttt
     4861 tgacgggctg gaccgattac gctttctcaa gcgacaacgt tgccgccgct caggcgggga
     4921 agactttgcg cccgcccgcg ctgcaagtca aaaacgcgcg tggggaatgg cagaccgtca
     4981 ttgaaaacat cggcatcccc gtcggtcgcc cgcagaccgt gcccgtcgat ttgacgggca
     5041 agttcctgac gacgaatcgt gaagttcgca tcgtcacgaa catgcgcatc tactgggatc
     5101 aagtgctggt tgatacttca agcacgcacg cgcccgtgcg gatgacgcgg cttgatccgg
     5161 cggtcgcgga tttgcgctgg cgcggctatt ctgcggaaga aacgcccgac gggcgcgagc
     5221 cgttcaccta cgcttacgag cgagtctctt acacctcgcc ctggaaagtg atgccgggac
     5281 gctacacgcg cgagggcgac gtgcgaaaac tgttgcgcac gacagacgac atgttcgtta
     5341 tcacgcgccc cggcgatgag atcatacttt cttttgacgc gaataaattg ccgccgctgc
     5401 ccgcaggctg gacccgcacg tttctgctct acgcggatgg tttcagcaaa gagatggaca
     5461 tcaactcggc cacgcccgac gggcttgaac cgctcccgtt ccacggcatg acccgttacc
     5521 cttactccgc gccggaagca tacccgctga cgcccgcgcg ccgtgcctat ctggaacgct
     5581 acaacacgcg catcgtcaga ggaagcctgc cgcagattga aaccgtgttc gccgaagata
     5641 tgatcttcaa cgcgactcga agcctcgaag gagaaaaata aaggcagtgg tttacatggt
     5701 agtgtgccga acacagcctc tcaccggcgg tgagctttgt ctcgatgaga tgttcacgcg
     5761 tgagggtgcc aacgacgtgt ccacaagtag cgcgccgcct agtgagagac tcacccctca
     5821 gacgcgccgc ataccccgga ttctacatgg ggcactcgcg cgctaaaaac cttgtcccaa
     5881 ggggtgtttc gggagtagca tacgccgcca tccgatagac cagactcaaa tctttcgaag
     5941 tcgcactcgc cagaagaaga tactgcgcgc cccttatcgg gaggggcgcc ccccgataac
     6001 gcttgtccca tttagaagga gaaccccgga tgaccgaacc cttagtcggc gacatagccg
     6061 aagacctgtc caaagtctcg aaaggcttgg gcgcgctcaa agaggtgtgg agtttcagca
     6121 gcagtgtggc cgcgctctca ggagcagtac aggttcaagg ccatccccat cgataagctg
     6181 ttcggcgcca tgttcggatt cggtgagaag ccaaacccgc tggcgttcgt cgagaagcat
     6241 ctcgtcgaga tcaacgccaa gctcgaccgc gtcctcgtgg gcatcgatga gatcaaggtg
     6301 gggatgctcg ggcaggcgat cctcgccgcc tacctcgaca tgtcagaccc cgtctacctc
     6361 atcaagaagt atttcgaaga cctcgcctct tacatggagg caggccacac gtttacggac
     6421 gccgagagga aagagttcgt cgagaaggtg ctcgggacga ggagcgagcg ggacggcgtg
     6481 gccttctgcg cgcacaagat agtcaagctc gcccccacgc agtccccgct cttcttcgag
     6541 ctactcttcc catacctggc cgcggcggcc actccgggca acgcccgcga gatgcgtgta
     6601 gccacgcttc gtagcggcgg ataacgccgg gcgttaggct gctccacttg actgacaaat
     6661 tattactgct tagggcttaa cctatagccc ggtacgggca tctccaacag agaaccccac
     6721 gtactcccat tcttaaacaa agttcctaga taggcagagt ccgataatta gaattatgta
     6781 cacaaggctt tgcgggttaa gtcctgtttg cagggaaagt ctgcaacctg cggggaaagt
     6841 caaccccctt caagttctag tttgaggcgg ttaatagcac tgtcaatcgc agtatgaaat
     6901 atctgtttac tgcaataacc ctcttcgctc caagtgctat acgcaggctt attgttgaag
     6961 gggcgactaa cacttcgtgt ggcggggtac tttctctctt tacgttttat atcggatggt
     7021 gaaatgcgga agttggtcag atcgtagtca gtttcacttt tcaagcggtc aaggatgccg
     7081 tgataactga tgatgctcgt ttctattacg tctgagtttg gaagattcgc ttttagtgat
     7141 tcaagatcgc tcttggcttg tctaagtttt gttctatcga atgaaggcgc agcaataacg
     7201 cgcggcggtt cattggagga aagcaaagcc ttagcttcta ggagagttaa aactttgcca
     7261 gttgcttcgt ttgttaaacg atagccatgt ttgcagggga tacatttgaa gaagccaata
     7321 tcagcgatac cagatacggg atgaataacc ggagcgagtt cggccagaca gacggggcag
     7381 tgaggttcaa ggtgttcatc ccacttaact gtgaactctt taatgaatgt tttcgccgtg
     7441 tctctttgct caattagttg tctctgggtt ttacgcagac ggaggacgta gaaaaacaat
     7501 gcgagatttg ctgatagcga tatgcctatt agcaccgcta acagcggtct gggcaatacc
     7561 ggcaatatac ggtcacgcac tggaggaact aacgccgaga ctagcgataa cagtaggggt
     7621 agtagtagcg caattgctct cgttgttagg tctgacgttg ctgtatctac tattgtcttt
     7681 ttaacttctg atgccatacc tatctcactt cccgcgccga tgctttgaga ggtttagcgt
     7741 ccttgcgcgg cttattccag taagggcttt tgcacttcgg acacacgcgc ggctcgttct
     7801 ctgtatcacg gggaacccat tcgtgagcgc atcgctcgca acgatagccc cagactttta
     7861 attgtacttt tgccatgatc tttatatacc ttcaaaataa tatactgtca agtcgttatt
     7921 tccttgacct gatatatacc tttggggtaa tatactactt agtagttagg ttgactaagt
     7981 ggaggccaaa gacaatggac gcacggcagc aaaaagggca agagatagcc gataaatcaa
     8041 acatcacgcg caaggggcat ctgtggctcg ttccgtcgca gaatggcagc ggcgcgtaca
     8101 ccgtcaacct agaatccgac aagccgcatt gcacctgccg cgaccatgaa tttcgccgcg
     8161 ccacctgtaa acatatcgtc gccgttcaag tcaccatcga acgcacaaag accgtcacca
     8221 ccaccacgct agaggccaca gacggacagg ctgctatcac ggagacagtc accacggaga
     8281 cggtcaaggt aaagcgcacc acctatcggc aagagtggaa gcaatacaac gcagcacagg
     8341 cttacgaaaa gtcagagttc cttgcgttgc tgtatggcct cacgaacggc gtagatgagc
     8401 ctgtgcagac gttcggcaga ccgcgcctgt caatggccga cactctcttt gccgtggtct
     8461 ataaaatcta ttcaacggtt tccactcgtc gctttgcctc cgatctgcgc gatgcttttc
     8521 agaaaggcta tctgtccaaa ctgccaagct acaacaccat tttcgactat ctgcaaatgc
     8581 ccgaactgac cccctacctg caaagcctca ttactgagag cagcaaggct ctgaaagccg
     8641 tcgagactga ctttgcggtt gatagttcgg gtttcagcac aggacagttt atgcgctgga
     8701 cggacgttaa atacgggcag aaggaagatg cgagacagtg gctaaaactt catctgatgt
     8761 gcggcgtccg caccaacgtt gttacctccg ttgaagtggg cggtgggtat tccagcgact
     8821 acaactattt caaacctctc gttgatagaa cagcgcagag tggctttacg atgaaggaag
     8881 tcagcgcgga taaggcgtac ttaggcacaa ctaactttgc agcgactttg aagcacggcg
     8941 cgatccctta catacctttc aagtcgaaca gccaacctaa cggcaacggc gcgttgtggg
     9001 cgcgtattta ccacttctac aacttccagc gcgaagagtt tttgcagcac taccacaaac
     9061 gctcaaacgt cgaaacgaca ttcagcatga tcaaggcgaa gttcggccaa cgcttacgaa
     9121 ccaagacacc aatagcgcag acgaacgaag cactttgcaa agttctctgc cacaacattt
     9181 gttgtgtgat tcagagcatc catgagttag ggattgagac gactttcaac gcagaaagtg
     9241 aggttgcacg gaaagtcggt tgatttaccg acttaacccg caaagcctgt acacaagggt
     9301 cttgcacaaa tattcattga aaactacatg gtgtgtgcat agatgcatag caacaaatga
     9361 atatgcacgt atgcctgaat atttgtgcaa gacctatggg ttatgcattg tgttatcgga
     9421 cacgatgcac aaggcattca ttaatgagtt agcaacccta gatgcttgaa gacaccctgc
     9481 gcgcttatac gctgcatata agggctaaaa tattttttgc aacactactt gtcgcggcag
     9541 gtcaatcttt tttgctgatg gtgctgatgt cgccgttacg ctccaagcgt gcctcttgga
     9601 cccggctgag gtctggagtt tgcccgttaa tgcgcagcgc ctctctcagg tcgtgctcgg
     9661 tgatgtgcga cttcctcatg gcgtcaggtt gcacctcgcc gtcgcacacg aggaggcggt
     9721 cgtgaccttt aaccattgtg gagaagccgt gcgcgcggaa ggctatcgcc gctaatgccc
     9781 agtggatgag aaccagcacg agactggccg caagggtggg caaaaacggc gcgttgccgg
     9841 tgatggcgcg gctgacgacg gagccgaaga tgatcccgag catgacatcg aaggccgtac
     9901 tccggcccat gaaacgcttg tccccaaccc ggagcatccc gatagccagg atgaagacga
     9961 tgaccgcgcg caggcacatc tgccacacgt tgatgtcttt gttctctatc cccagaccca
    10021 gagccgcatt gatgacctgt cgtatctctt ccatttgacg cccccttgtt tgtgatcact
    10081 ttaacgacag catataccgt gagactcatt cctcctagct caatcccggt aacccatcat
    10141 tttttgccct gcccgacggg aacgatggcg atctcgccgt cgcgctcgac caccacgcac
    10201 ttgatctgct cgatcctaag accacctagt ttttacgaat agcccttgag atgaaatgac
    10261 gcaccgccgc acctctcatc ccgagcaatg cgtatgtgat gagggctgca ccgacaaccg
    10321 taccgatcac caggaagaag gtggcgaggc tctctaccgg cgacgttacg gccaacgggc
    10381 tgaagccgaa cacagacgaa atcccgagcc acgccgggat gagtgcgacc tgtgaggtgg
    10441 cagccagccc gatcatctca cgcctgccca cgtcgtcggc ggtcgctaaa ctggcggcca
    10501 ctccgatggc gagcgagatg agaaaggtcg cgctcaacga actgctcggc tcgaagcgca
    10561 taggcggtcc cgtcatgagc gccaccacga tgctccccgt cgctgtcaga cccgtagcta
    10621 ccagaagcgc gagtatcccc tgcccggcaa gtcgccattc tctcgtccac agcccgaacc
    10681 cgacggcgag cagcaagggc aggaacggca tgaagagtag accggcgata atcaggagga
    10741 ggttggattg gatcatccca taagacaaaa gcagcgcgcc gatgaaaaaa cggacgacga
    10801 agctgaaggt gagatggctg aattgccaca ggtcttcaag gatgtctact gtcggcacga
    10861 ccaccggacg cgtgacttct aacggcgatt cttttgaaac gatggagagc ggctggcgga
    10921 ttgtgatcga atacgtgccc ggttc
//
LOCUS       DASWNS010000394        13592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_224692, whole genome shotgun sequence.
ACCESSION   DASWNS010000394 DASWNS010000000
VERSION     DASWNS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13592
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224692"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3327)
                     /locus_tag="VGB76_13735"
     CDS             complement(<1..3327)
                     /locus_tag="VGB76_13735"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEY0100005.1"
                     /translation="MRFATYRITAHGLDSRDARAGIQSSSVRFGDELLVYAPDSEWQS
                     ISVRAGASGLVLQEQAGEFEKEELHLVVQVGDSFRQQHPRVPVLLNKGRYLAVSLKPE
                     QARRLAAKKSCCYTIHPLEAGEVVFELQPPPAERAQPEAWLKTLVSRLSRPHVEATLQ
                     HLVSFPTRHSTSTHYLAAAVWARAQLAAMGYNTRLQTVKLDGGKSLNVIAEKAGQSSG
                     ARGLVVVTAHLDSINREGDAAPAPGADDNGSSAAGLLEIARVLKEHAGAHDLRFVLFG
                     GEEQGLHGSDQHVAALTTNERARIRAVVNMDMIATLNTTQPTVLLEGAPVSRSVIEGL
                     AAAAAAHTKLFVQTSLKAADSDHVSFIRAGIPAVLTIEGADRTNQNVHTEHDTLLHIN
                     YDLLLEILRMNVAFVATAVGRQGGIPMFDSHTPTQDENRLDAAFAEEQRAPAFQFSGC
                     YKYNGGASSREGRSFTDHASGQSMAAIENPIYRLDEPVYLDSPEEYDEAERAPDQLRF
                     TISVDIDGSDPLNIVSGTVALGPDTPGVASPHFIGRVASNTAAGTGRILTVRDFEFRW
                     PNSNDSINQITVELTRASPANPSAKLTFSDTVLNHHHGPYAVARSSPYFHEVEMDIDR
                     EMNAVDVEPYDTHTHPDRPSNLPRESLTLESAFAKAGIRITRSQGSGTVVNTSSAGTD
                     RRWSYSELHDSMQLHWDAFANKPQWKMWLFLAELAESDSLGGVMFDGEINEPGGVDRQ
                     GTAIFTKCPFFHTPGGGYISANPPVDEAVKRELFFNLMHETGHAFNLAHSFQKEQGGV
                     WRPPAWMPIKTDNQSLSWMNYPDQATPVPGPTPNATWFYRRFRFRFDRSELLFLRHAP
                     ETYVEMGAQAWFKNHGRVARINLDPRLELIVRSRQTVTHVGRAVTFFELGEPVILELR
                     LRNVSSQPITAHANLDPSDGLVEVAVTNPRGERWPFLPIDHTRTLMQLLTLEPGGQSV
                     YGQLDLTMGSFGFPFKEPGAYRVEVSYTNVDGSTAAAVMQLYVRPPAQYDAVPIVNEL
                     FDARVGRTLYVDGTRVMEDVNDKLGWVGQRLRDVLGERNPISMHLSVVQCKPLATDGK
                     YIDPDTSELRAGGEEPDRVVA"
     gene            4370..4897
                     /locus_tag="VGB76_13740"
     CDS             4370..4897
                     /locus_tag="VGB76_13740"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEY0100006.1"
                     /translation="MKSDYISETPYWYAIHTKPRQEERAACNLIAWEVETFSPRLKES
                     RRNTFTGQPMQLVKPLFPGYIFAKFDARTMLHKVCFTRGVHSVVSFGGSPVPVSDEII
                     SLIRSQIGADGFVKLGGDLDVGDEVRIKDGPLQNFVGVFERKINATNRVMLLLRTVNY
                     QGRVEVDLALLEKIS"
     gene            complement(5010..5348)
                     /locus_tag="VGB76_13745"
     CDS             complement(5010..5348)
                     /locus_tag="VGB76_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100007.1"
                     /translation="MSVAANARLARAANCLLADAGRCTIVGRDGASVGGELVAARRQM
                     FGLEPPVVELDETVGAVLVAVAMSDRDDAVVARLRVEVRQAVQLVEEKEAGEDDGEIL
                     AVSHLDSERP"
     gene            5477..5701
                     /locus_tag="VGB76_13750"
     CDS             5477..5701
                     /locus_tag="VGB76_13750"
                     /inference="COORDINATES: protein motif:HMM:NF015578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HEY0100008.1"
                     /translation="MSRDEREDTTIYKVVVNHEEQYSIWPADRENALGWNDAGKSGTK
                     AECLEYIKEVWTDMRPLSLRKKMEEAANQS"
     gene            5856..6221
                     /locus_tag="VGB76_13755"
     CDS             5856..6221
                     /locus_tag="VGB76_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEY0100009.1"
                     /translation="MPVNTATTDGFESTAPKPTSRLRLFCFPYAGGGSNIFRNWPRQL
                     PSDVEVVTPLPPVCEGRFREPPLDRIERVIDAVTPDILPWLDRPFAFVPRERLEPWRV
                     QSQAGYALHMLSRAHFFFN"
     gene            6384..7211
                     /locus_tag="VGB76_13760"
     CDS             6384..7211
                     /locus_tag="VGB76_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY0100010.1"
                     /translation="MLTALTRGVSPSLGNCQLTYMNRECIDVEQAARQHRAYEESLRE
                     LGLRVVSLPLQPELPDAAFVEDLAIVLDEVAVIARMGAVERQPEVESLAAVLARYRPL
                     RFLDAPATLEGGDEIKVKRTIYVGVSCRTNESGVAQLQSALRPFDYEVRQVGVEGCLH
                     LTTGASYIGENTILINPAWVDASQFGGFELIETPESEPWSANSILVEDVVLLSASYPL
                     TCERLAARGFKVRTVDISELEKAEAGLSCMSLTFEAPPSSSEETDLARQLNVARPSE"
     gene            7385..9169
                     /locus_tag="VGB76_13765"
     CDS             7385..9169
                     /locus_tag="VGB76_13765"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HEY0100011.1"
                     /translation="MKVTSHSTLIPPYGDQLVDLLAGDAREELREHATRLPSVQLSER
                     AVCDLELLATGAFSPLKRFMGREDFRRVVEEMRLASGHIFPVPVTLPVKPEGEWSKSE
                     EVALRNSKNELLAVMTVEEVYEWDLDDTARKVFGTTDLRHPLVAEMHRWGKTNISGRL
                     QVLQLPRHYDFLDLRLTPAETRARLETFGHPNVVAFQTRNPLHRVHEELTKRATQEVD
                     GVLLLHPVVGLTKPGDVDHYTRVRIYKALAKSYYDSDRLLLSLLPLAMRMAGPREALW
                     HAVIRRNYGANYLIIGRDHASPGVDSEGRPFYGPYDAQELVESLAEEVGVRVKPFNEL
                     VYLPDEAQYVESTRIPERARTASISGTQVREEYLDAGRKLPDWFTRPEVAEILADSYP
                     PRHKQGVCLWFTGFPGAGKSTTADVLTVLLLEHGRHVTVLDGDVVRTHLSKGLGFSKE
                     DRDINIRRIGYVAAEIVRHGGVVICAAVSPYRATRNEVRTMVGTDKFVEVFVDTPLEV
                     CEQRDTKGMYAKARRGEIRDFTGIDDPYEPPLHPEVSIETVLRTPEENARRILHFLIQ
                     SGFVKVAKETELNEPKGDSEKAVPAQEA"
     gene            complement(9319..10095)
                     /locus_tag="VGB76_13770"
     CDS             complement(9319..10095)
                     /locus_tag="VGB76_13770"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY0100012.1"
                     /translation="MRLPPHDQQVWLSPPERTLLADGEVHVWRAELNPDGSVLGELAE
                     ALSPDERLRASKFHFQRDRDHFVAARGRLREILSRYAGTAAGRLRFSYDKYGKPSLVG
                     EAGGGALRFNVSHSNGVALYAVTVGHEVGVDVEFVREDFASLEIAERFFSRREVAALL
                     ALAPGQRASAFFDCWTRKEAYIKARGEGLSHPLHLFTVSLAPGQPAALLYTEDDEQEA
                     ARWSLVELFPGAGYRGALAVEGGAPSLRCWSCEGAGSRIE"
     gene            11252..11626
                     /locus_tag="VGB76_13775"
     CDS             11252..11626
                     /locus_tag="VGB76_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100013.1"
                     /translation="MKAYSLDLRQRVLSAALRGDRTIPQVAGLFGVSTAFVNKMRRLH
                     RTGVDLMPRPHGGGYPSRLLPRHEKLLRAEVRRRKDSTLEELRAHLAVGDGLAVSVST
                     VSRALIRLDLPRKKKSGGERAE"
     gene            11682..12194
                     /locus_tag="VGB76_13780"
     CDS             11682..12194
                     /locus_tag="VGB76_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0100014.1"
                     /translation="MFVDETAVDTAMTRRSGRAPRGERVCDSAPRNYGVHPSVVSAMG
                     RRGLVPTLTVGGAVETRCFDTYLERVLGPRLRRGDMVVLDNLGAHRASRIEEVAEGRG
                     AEVLWLAPCSPDFSPIEQCWSKIKSYLRGVKARTADELNEALAKAIKLVRKADIRGWC
                     KHCGYSLSRK"
     gene            12568..>13592
                     /locus_tag="VGB76_13785"
     CDS             12568..>13592
                     /locus_tag="VGB76_13785"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100015.1"
                     /translation="MVVNEITGLDEAINAYKAALDNIDNIPLIRSTNSSQRGEDLQKV
                     LSVLIARDKLAHLLSNAIVNPTEMINVVEQDQRLRNDAKKIVSVVGQSALAHWRDAKQ
                     TLKKPDEPISFPWWWSLDEQVAEPWFIYALKTCLWLCIAVALSFTVEIVRRFVSSGVD
                     LPSTIAQGLLALLVGGTLIQFARQLVEGISRQGGKKLQLKWRTQLILAVVLVTVAVVL
                     ELSRAKVVAHYSNMGVDQLNAGQLAGAIQNFQRGISLKPNDAIAHFNLASAYESVLDY
                     DKAEAEYRAAIQWNKQLSPAYSKLARLYILRRNDPGAALMLLRAGLRETDAQSLGEGP
                     TDDNWKR"
BASE COUNT         2773 a         4020 c         4110 g         2689 t
ORIGIN      
        1 ggccaccacg cggtcgggtt cttcccctcc ggcgcgcagt tcgctcgtat ccgggtcgat
       61 atacttgccg tccgtcgcca gaggtttgca ctgcacgacc gacaggtgca tggagatcgg
      121 attgcgctcg ccgagcacgt cgcgcaatct ttgtccaacc cagccgagct tatcgttcac
      181 gtcctccatc acgcgcgtgc cgtccacgta gagcgtgcgc ccgacccgcg cgtcgaagag
      241 ttcgttgacg atgggcaccg cgtcgtattg cgccgggggg cggacgtaaa gctgcatgac
      301 ggcggcggcg gtgctgccgt caacgttcgt atagctgacc tcgacgcggt acgcgcccgg
      361 ctccttgaac gggaaaccga agctgcccat cgtcaggtct aattggccgt agacggactg
      421 gccgccgggt tccagcgtca agagttgcat cagcgtgcgc gtgtggtcga tgggcaggaa
      481 cggccaacgc tcgccgcgcg ggttggtgac ggcgacctcg accagaccgt cgctcgggtc
      541 gagattggcg tgcgccgtga tgggctggct gctgacgttg cgcaggcgca gttcgagtat
      601 caccggctcg cccaactcga agaaggtcac cgcgcgaccg acgtgggtga cggtctgcct
      661 gctgcggacg atgagttcga ggcgcgggtc gaggttgata cgcgcgacgc gcccgtgatt
      721 cttgaaccaa gcctgcgcgc ccatctccac gtaggtttcg ggcgcgtgtc gcaggaagag
      781 caactcgctg cggtcgaagc gaaagcggaa acgcctgtag aaccatgtgg cgttgggcgt
      841 ggggccggga accggcgtcg cctgatcggg atagttcatc catgagagcg attggttgtc
      901 ggtcttgatc ggcatccacg cgggcggtcg ccagacgccg ccctgctcct tctggaaaga
      961 gtgtgcgaga ttgaaggcat ggcctgtctc gtgcatgagg ttgaagaaga gttcgcgctt
     1021 gacggcctcg tccacgggcg ggttggcgga gatgtagccg ccgccggggg tgtggaagaa
     1081 agggcacttg gtgaagatgg ccgtgccttg acggtcaacg ccgcccggct cgttgatctc
     1141 gccgtcgaac atcactccgc cgaggctgtc gctttcagcc aactcggcga ggaagagcca
     1201 catcttccac tgcggcttgt tggcgaaggc gtcccagtgc aactgcatcg agtcgtgcag
     1261 ttccgagtag ctccagcgcc ggtctgttcc cgcgctgctc gtgttcacca ctgtcccgct
     1321 gccctgcgag cgcgtgatgc ggataccggc tttggcgaag gccgactcca gtgtgaggct
     1381 ctcgcggggc aggttcgaag ggcggtcggg atgcgtgtgc gtgtcgtaag gctcgacatc
     1441 gacggcgttc atttcgcggt caatgtccat ctcgacctcg tggaagtagg gcgacgaacg
     1501 cgcgacggcg taagggccgt gatgatggtt gagcacggtg tcggagaagg tgagtttggc
     1561 cgaggggttg gcaggggatg cgcgcgtcaa ctcgaccgtg atctggttga tcgagtcgtt
     1621 gctgttaggc cagcggaact cgaagtccct gacggtcagg atgcgccctg tgcccgccgc
     1681 agtgtttgag gcgacgcgcc cgatgaagtg ggggcttgcc acgccgggcg tgtcgggtcc
     1741 aagcgcgacg gtgccgctga cgatgttgag cgggtcggag ccgtcgatgt ctacgctaat
     1801 ggtgaagcgc agttggtcgg gcgcgcgctc ggcctcgtca tactcctcag ggctgtcgag
     1861 gtagaccggc tcgtcgagac ggtagatcgg attttcgatg gcggccatgc tctgccccga
     1921 agcgtggtcg gtgaaactgc gcccctcacg ggagctagct ccgccgttgt acttgtagca
     1981 cccgctgaac tggaaagctg gcgctctctg ttcctctgcg aaggcggcgt caagtctgtt
     2041 ttcgtcttgt gtcggtgtgt gtgaatcaaa catcggaatg cctccctggc gacctactgc
     2101 ggtggcgacg aaagcgacgt tcatgcgcag gatttccagc agcaggtcgt agttaatgtg
     2161 caagagcgtg tcgtgctcgg tgtggacgtt ctggttagtt ctatctgccc cctcgatggt
     2221 gagaacggcg ggtatccccg cccggatgaa ggagacgtga tcgctgtcgg cggccttgag
     2281 gctcgtctgc acgaagagct tcgtgtgagc ggcggcggca gccgctagcc cttcgataac
     2341 agaccgggag acaggcgcgc cttcgagcag gacggtgggc tgcgtcgtgt tcagggtcgc
     2401 aatcatgtcc atgttgacga cggctctaat tcgtgcccgc tcgttcgtcg tgagcgcggc
     2461 gacgtgctgg tcgctgccgt gcagcccctg ttcctcgccg ccgaagagga cgaagcgcaa
     2521 gtcgtgcgcg cccgcatgct ctttcagcac gcgtgcgatt tcgagcagac ccgccgcgct
     2581 gctgccgttg tcgtctgcgc cgggagcggg cgcggcgtcg ccttcgcggt tgatggaatc
     2641 gagatgcgcg gtgacgacga cgaggccgcg cgcaccggac gactgcccgg ccttctctgc
     2701 gatgacgttc aggctcttgc cgccgtcgag cttgaccgtc tggagacgcg tgttatagcc
     2761 catcgcggcc agttgcgccc gcgcccacac ggcggcggcg agataatgtg tgctggtgga
     2821 gtggcgcgtc gggaaagaaa ccagatgttg gagcgtggcc tcgacatgcg ggcgcgagag
     2881 gcgtgacacg agcgtcttga gccatgcttc aggctgcgcg cgctcggctg gcggcggctg
     2941 caactcgaag acgacctcgc ctgcttcgag cgggtggatc gtgtagcagc acgatttttt
     3001 ggcagccagt ctccgcgcct gctccggctt cagcgagacg gccagatagc gacccttatt
     3061 gagaaggaca gggacgcgcg ggtgttgctg gcggaatgaa tcaccgacct gcaccacgag
     3121 gtgcaattct tccttctcaa actctccggc ttgctcctgc aacacaagac ccgacgcccc
     3181 cgcacgcaca cttatcgact gccattcgga gtcgggagcg tagacgagta attcgtcgcc
     3241 gaagcgtacg gaactgctct gtattccggc gcgtgcgtcg cgcgagtcca gaccgtgggc
     3301 tgtgatgcga tatgtggcga acctcataag taacacctga tttctgctcg ccgctttatg
     3361 gttggcacgt aacttcccgt tgaagacttc gacggcggct tatgtcggaa tgctaaattc
     3421 ataccacttg gcgttcagcc tccagccttg cgcgagcggc gggaataagt acgtaacccg
     3481 aaagggaata gcgtcgcggc gtgagagacg cgggagcgca tctactgcaa cagccgcccg
     3541 ccgcacccta tcaattctga tagccacact atcttttctg atatgacgtg tatgaacgcg
     3601 gcgacccgcc ttgagaaccc atgctgtcgg ttgatgactt gttcgggagg ttcactccct
     3661 gaccgcgctg aaatggtgcc ggacgacacc attttactct gcggagaagg ggtgcacacg
     3721 ttttcaactc aagaccggga tgaatttaag gcccgtaata tcgggctgcg agacggcgaa
     3781 attgcgttta tttatcaggc gtaattcggt ggtaaatggt gctacgaaaa ggaaaacacg
     3841 gcctgtcaat taatacccaa gactatcttg acaattagag ggcggtgcgt gtaacgttgt
     3901 gcctgcacga atcggttaag aagagaaggt tagtgccgcc cttaattctc tatcgcccct
     3961 tactcaactt gagtctttag ctgaatcagg ccgaaatgat cctgcgtagc agagattatt
     4021 ttccggcagg ttgattctgc tctgtgtagg aacacggtcg tgagaagaca cgtgggtcat
     4081 aaacccgcac cctcgggctg gctgtagttt tccactcccg atctccggtt ccgtgctgac
     4141 ccggcacagg gcgaaggagt atctgtctga tatgagccgc ccgaccggag actttcctcg
     4201 ctctccggtt cgctcaacgt tcttataact gttcagcgcc ccgacgttcg aagggaatta
     4261 ggggcagcgg gtttttaatc ctcgacggac gcgtgcgcat ttcccgaaac cggagtagta
     4321 ccggctgaac tgcttcccaa cacgtttttt gacgtaaaga tgcctctgta tgaagagcga
     4381 ttatatcagc gagacgcctt attggtacgc catccacacg aaaccccggc aggaagaacg
     4441 cgccgcgtgt aacttgatag cgtgggaagt agagactttc tctccgagat tgaaagagag
     4501 ccgccgcaac acgttcaccg gacagccgat gcaactcgtc aagcccttgt ttccgggcta
     4561 catctttgcc aaattcgatg cgcgcacgat gctgcataag gtctgcttca cgagaggcgt
     4621 gcatagcgtg gtcagcttcg gcggctcgcc tgtgccggtg agcgacgaga tcatctcact
     4681 catccgctcg cagatcgggg cggacggctt cgtgaaactc ggtggcgacc tcgatgtcgg
     4741 tgacgaggtg cggatcaaag atggtccttt acaaaacttc gtgggagtgt ttgagcgtaa
     4801 gataaatgcg accaaccgtg tgatgctgtt gctgaggacg gtgaattatc aggggcgggt
     4861 cgaggttgac ttggcgttgc tcgaaaagat tagttgaatc ggattattca tccgagcgaa
     4921 tggatgcttt gtttgagggg agccggagtt ggcacgcgca ggccgcaagc gacaacaggg
     4981 ttccaacctt ctgacaatca ccctacatcc tacggacgct cgctatccag atgtgaaacc
     5041 gccagtattt ctccgtcgtc ttctccggct tctttctctt cgacaagctg cacggcctgc
     5101 cgaacctcga cgcgcaggcg cgcgacaaca gcgtcgtcgc ggtcactcat ggcgaccgct
     5161 actaacacag cgccgaccgt ctcatcaagc tcgactacag gcggctccag tccgaacatt
     5221 tgacggcgcg cggccacaag ttcgccgccg actgaagccc cgtcgcggcc aactatggtg
     5281 caccggccag cgtccgcgag aagacagttc gcggcgcggg caaggcgcgc atttgcggcg
     5341 acgctcaagt ttgtgtggag catctaccgg aggttaaagc gcaagtgggt gtttgcaagc
     5401 ttcgccgact tcgtgccggc gaaacagtag agcacctgtg gattagagat tcatcacatt
     5461 catcttagga gacgttatga gcagagacga gagagaggac acaaccatct acaaggtcgt
     5521 ggtcaaccac gaagagcagt attcgatctg gcctgccgac cgtgagaacg cgctcggctg
     5581 gaacgacgca gggaagtccg ggacgaaagc cgaatgcctc gaatacatca aggaggtctg
     5641 gacggacatg cgcccgctca gcctgcgtaa gaagatggag gaggcggcga atcaatcgtg
     5701 atcgcggcga ggggcgcggc ggggagttcg tcctgtaaag accgaaccgc cgcgcccgga
     5761 tgccgccgtc gccacccgcg cccggccaaa acgtgagaga cgacgcaacc aactcaaccc
     5821 cggcgcagat gccgggcagc aaactgtttt aagttatgcc ggtcaacaca gccaccaccg
     5881 acgggttcga atcaaccgcc ccgaagccta cctcacgtct acgcctcttc tgtttcccct
     5941 acgcgggcgg cggctcgaac atcttccgca actggccgcg acagcttccg tccgatgtcg
     6001 aagtggtgac gccgctgccg cccgtctgtg aaggccgctt ccgcgaaccg ccgctcgacc
     6061 gcatcgagcg tgtgatcgac gcggttacgc cggacatcct gccgtggctc gaccgccctt
     6121 tcgccttcgt gccgcgcgag aggctggaac cgtggcgcgt gcagtcgcag gcgggctacg
     6181 cgctgcacat gctctcccgc gcgcacttct tcttcaacta gcgacaggcg gacatcctgc
     6241 gcgtcgtctc gctcaaactt caaccacagt tgggcggtgc cctctgagcc gcgcctcgcg
     6301 ccgccgcacc gcaccgcctc gatagtctcc cgcacacgtc gtcgctcgac agagagcgac
     6361 caaccgagag gagagaaacc gccatgctta ccgctttgac gcgcggcgtc agtccgagcc
     6421 ttgggaattg tcagttgacc tacatgaacc gtgaatgcat cgatgtggag caggctgccc
     6481 gccagcaccg cgcttacgaa gagagcctgc gcgaactagg cctgcgcgtc gtctctctgc
     6541 cgctccagcc ggaactgccg gacgccgcat tcgtcgaaga cctggctatc gtgttggacg
     6601 aagtggccgt catcgcgcgg atgggagccg tcgagcggca gccggaggtg gagagcctcg
     6661 cggcggtgct cgcgcgctat cgcccgctac ggttcctcga cgccccggcc acgctcgaag
     6721 gcggcgacga gatcaaggtc aaacgcacga tctacgtcgg cgtctcgtgc cgcacgaacg
     6781 agtccggcgt ggcgcagttg cagagcgcgc tcaggccgtt cgactacgag gtcaggcagg
     6841 tcggcgtgga gggctgcctg cacctgacga ccggcgcgtc ctacatcggc gagaacacaa
     6901 ttctcatcaa cccggcgtgg gttgacgcgt cgcagttcgg cgggttcgaa ctcatcgaga
     6961 cgccggagtc cgagccttgg agcgctaaca gcatcctcgt cgaggatgtg gtgctgctct
     7021 ccgcctccta cccgctgacg tgcgaacggc tcgcggcgcg tggcttcaaa gtccgcaccg
     7081 tggacatctc cgaacttgag aaagcggagg ccgggctgag ttgcatgagc ctcacctttg
     7141 aagcccctcc gtcgtcgtct gaggaaacag acctcgcgcg gcagttaaat gtcgcgcgac
     7201 cgtctgagta gtcacagaag gaaaaatagg atgaacgaac gttacacacg cctctgatct
     7261 ggcgtcgaac gttcgcgcaa cctctgcgag tcgtagacga gttacgcaaa ggcttcctga
     7321 acctcgtgtc catggattta tactcagatg aacaaggaca agacaggaag cgaacgtaac
     7381 tgacatgaaa gtaaccagcc attcgaccct cattccgcct tacggcgatc aactggtaga
     7441 cctgctcgca ggcgacgcgc gcgaggagtt gagagagcac gcgacgcgtc tgccttcggt
     7501 gcagctttcg gagcgggctg tgtgcgatct ggaactgctc gccaccggag ccttctcgcc
     7561 gctcaaacgc ttcatggggc gcgaggactt ccgccgcgtc gtcgaagaga tgcggctcgc
     7621 aagcggccac atcttccccg tccccgtgac gcttccggtc aagcctgaag gggagtggtc
     7681 gaagtccgaa gaggtcgccc tgcgcaactc gaagaacgaa ctgctcgcgg tgatgaccgt
     7741 cgaagaggtc tatgaatggg acttggacga cacggccagg aaggtcttcg gcacaaccga
     7801 cctgcgccat ccgctcgtcg cggagatgca ccgctgggga aagaccaaca tctccggtcg
     7861 cctgcaagtc ttgcaactcc cgcgtcacta cgacttcctc gacctgcgac tgacgcccgc
     7921 cgaaacccgc gcgcgcctgg agacattcgg gcacccgaac gtcgtagcct tccagacgcg
     7981 caacccgctt catcgcgtgc acgaagaact gaccaagcgc gcaacgcagg aggtggacgg
     8041 cgtgctgctc ctgcacccgg tcgtcggcct caccaaaccc ggcgacgtag accactacac
     8101 gcgcgtccgc atctacaagg cgctggcaaa gtcctactac gactccgacc gtctcctcct
     8161 ctcgctcctg ccgctcgcca tgcgcatggc cgggccgcgt gaggctctct ggcacgccgt
     8221 catcaggcgc aactacggcg caaactacct gatcatcggg cgcgatcacg ccagccccgg
     8281 ggtcgattcc gagggcagac ctttctatgg tccttatgac gcacaggaat tggtcgagag
     8341 cctcgccgaa gaagtcggcg tgcgcgtgaa gccgttcaac gaactggtct acctgccgga
     8401 tgaggcgcag tacgtggaat cgacgcgcat cccggagcgc gcgcgcaccg cctccatctc
     8461 cggcacacag gtgcgcgaag agtatctcga cgccgggcgc aaactgcccg actggttcac
     8521 ccgtcctgag gtcgcggaga tacttgcaga ctcgtacccg ccgcgccaca agcagggcgt
     8581 ctgcctctgg ttcacaggtt tcccgggcgc aggcaagtct acgaccgccg acgtgcttac
     8641 agtcttactc cttgagcacg gtcgccacgt gacggtgctc gacggcgacg tggtgcgcac
     8701 gcacctctcg aaaggcttag gcttcagcaa ggaggacagg gacatcaaca tccgccgcat
     8761 cggctacgtc gctgcggaga tcgtgcgcca cggcggcgtc gtcatctgcg cggcggtcag
     8821 cccctaccgc gccacccgca acgaagtgcg cacaatggtc ggcacggaca agttcgtgga
     8881 ggtcttcgtg gacacccccc tcgaagtgtg cgagcagcgc gacaccaagg gcatgtatgc
     8941 caaggcgcga cgcggcgaga tcagagactt caccggcatt gacgatcctt acgagccgcc
     9001 gctgcatcca gaggtttcta tcgagactgt gctccgaacg ccggaagaga acgcgcggcg
     9061 gatactccac ttcctgatac agagcggatt cgtcaaagtc gccaaagaga cggagctaaa
     9121 cgaaccgaaa ggcgactccg agaaagctgt cccggcgcag gaagcataag catcgcggac
     9181 acgagttact tcaactcacg caggcacaca gagagagagg gcgcaggaga cgcacacatg
     9241 tgtctcctgc gccctcggtc tccgtctaca gcgacgttgg aatcctatct cccatctccc
     9301 atctcaaatg aaaagcagct actcgatgcg actgcctgcg ccctcgcaac tccagcagcg
     9361 cagagagggc gcgccaccct ccaccgcgag agcgccacga tagcccgcgc cgggaaacaa
     9421 ctcgaccagc gaccaccgcg cggcttcctg ctcatcgtcc tccgtgtaca ggagcgcggc
     9481 gggctgaccg ggcgcaagcg acacggtgaa caggtgcagc gggtgcgaaa gcccctcccc
     9541 gcgcgccttg atgtacgcct ccttgcgcgt ccagcaatcg aagaacgcag acgcacgctg
     9601 acccggcgcg agtgccagca aagccgccac ctcgcgccgg gaaaagaagc gttcggcgat
     9661 ctccagactg gcgaagtctt cgcgcacgaa ctcgacatcg acccccactt cgtgccccac
     9721 cgtcacggcg tagagcgcga cgccgttcga gtgcgagacg ttgaagcgca atgcgccgcc
     9781 gcccgcctcg cccacgagcg aaggcttgcc gtacttgtca taagagaaac gcagccgtcc
     9841 tgccgcagtg ccggcatacc ggctcagaat ctctctgagc ctgccgcgtg cggccacgaa
     9901 atgatcccga tccctctgga agtgaaactt gctcgcccgc aatctctcat ccggagaaag
     9961 agcctccgcc agttcaccca gcaccgagcc atccggattc agttcagcac gccagacgtg
    10021 cacctcgccg tccgcgagaa gcgtgcgctc cggcgggctg agccatactt gctggtcgtg
    10081 tggcggcaat cgcatctccg ttcaataaac tagagcgtca cgcctattct gatgatgctg
    10141 aacggtgatt atcgtcagtg tgccaaagct cacgccgtgc acaccatcca tgctgagttt
    10201 ctacccctgg gcattcacct tcgtcaagcc accctcaatt ctgggagcgc aacgacatga
    10261 gggagggaga aagtccctca accattgagc aagtcggatc cctgattccc ggaagtgact
    10321 tcgagtctga gccgttggac ggtaatacac ctagcgactg gcctgaagat gtcgcggatg
    10381 cagctgagga tgctgaggat tcgattccgt cggagcaatg gagttggtgg tggcagaggt
    10441 acgttcgcca ttaccgttac tcgcctccat actcaccaga gttctgggat atttttcagg
    10501 gagcgattga ggcaggggag cgtattaagc gcatcgcacg attagcttac gcgaacggtg
    10561 tacccgtgcg gtggtttcaa tatgacccgg gaagtgcaga cagtggcgtc cttgacttac
    10621 ttcacgtcct gagtgcccgg ccagtggtca gagtcttccc ctctggcact ctcttcagcc
    10681 gtctagcttt gacgacagta aataaggaaa gtcttgttct ctattcgcca agagaccatc
    10741 aatcacctgg cgtacttttc tgtgcagact cagaccttca aggggtttca cttccgttgc
    10801 gcccgcgtga tttgattacg gttcctcatc atggggccga agctaaccag gacgtgtaca
    10861 ttgcagtcag caccgcattg ggtgaaaacc tgaatacact cacatgggta cgcagtgact
    10921 cgcgcgtgaa aaagcgtccg tgttctgcct accgtggatt gccggggagg aagttttgca
    10981 cagtttgtcg tactggcctg ccgaagcagc gggtgacact cgtcggtagg ggaggcgtct
    11041 gggctcgaca ggctaaggta cggtcttgtg agtgttgagg tgaggctctt cgctgggtca
    11101 caaatccgca aaatgcggtg gcatattctg ctcggaatct aagacaataa gcttgcgaca
    11161 ttactcacga aatatcaaca tttgaaagat tcggacttct atagctcttc gcacttgagt
    11221 gaacacggcg actgaagtaa cttgccgcgt catgaaagct tattcactcg atttacggca
    11281 acgggtgctc tcagcagccc tgcgcggcga ccgtaccatc ccgcaagtcg cggggttgtt
    11341 tggtgtcagc accgccttcg tcaacaagat gcggcggcta caccgcaccg gagttgacct
    11401 catgccccgt ccgcacggcg gcggctaccc gtcccgcctg ctaccccggc atgagaagtt
    11461 gctgcgcgcc gaggtccggc gtcgcaaaga ctcgacgctc gaagaactgc gcgcccacct
    11521 cgcggtgggg gatggcttag cggtgagcgt ctccacggtt tcgcgggcgc tcatccggct
    11581 cgacttgccg cgtaaaaaaa agtctggcgg cgagcgagcg gagtaactac aagcggggct
    11641 ggttccgccg ccgtgcccgc ctcgtcaacc atcgtcgcct catcttcgtt gacgaaaccg
    11701 cggtcgacac ggccatgacg cgccgctctg gtcgggcacc gcgtggcgag cgcgtctgtg
    11761 acagcgcgcc gcggaactac ggagtgcacc cgtcggtggt cagcgcgatg gggcggcgcg
    11821 ggctggtgcc gacgctcacg gtcgggggcg cggttgagac gcgctgcttc gacacctact
    11881 tggagcgggt gcttggcccc cgcctgcggc gcggcgacat ggtagtgctc gacaacttgg
    11941 gcgcacaccg tgcgagtcgc atcgaggaag tagccgaggg gcgcggcgct gaggtcttat
    12001 ggctggcacc ctgctcgccc gacttcagcc ccatcgagca gtgctggtca aagatcaaga
    12061 gttacctgcg tggggtgaaa gcccggacgg cagatgaact gaacgaggct ctggcgaagg
    12121 ccatcaagct agtaaggaaa gccgacatcc ggggctggtg caaacattgc ggttactcac
    12181 tctcacgcaa gtgagaaccg ctataatgta ctgtttactt atgtttagtc atccctcttc
    12241 aaacttccct tgacagataa cacgagtcga gtgaacattc cgttgtttca atggagaaga
    12301 aggctttcgt caaagtctaa tcggatttca atgacctctt gaaatcagaa tccacctcag
    12361 ccattcatca caagctgctt tagagctgct atgcgcgtta cgtaattcac agcaatatca
    12421 tgcctgacgc catagcttag tttagtaagt cattctctac tcaggaaaag cggcaaaaca
    12481 ccgccggctt tatattcaca ctagtcacgg agcaatttct ctgtccattt cgccagaaac
    12541 tctctgctct ggcttatcct aaacgcaatg gttgtaaatg aaataacagg attggatgag
    12601 gcaataaatg cttataaagc agccttagat aacatagata acattcctct catcagaagt
    12661 acaaattcgt cccaaagagg tgaagatctt caaaaagtac tgagcgtact cattgctcgc
    12721 gataagctag cgcaccttct atcaaatgcg atagttaatc ctacagagat gattaatgtc
    12781 gtcgaacaag atcaacggtt aagaaatgac gcaaaaaaaa ttgtcagcgt tgtcggccag
    12841 tctgcgttag cccattggcg ggacgcaaaa caaacattaa aaaaacccga cgaaccgata
    12901 agctttccgt ggtggtggtc gcttgacgag caagttgctg agccatggtt tatatatgct
    12961 cttaagacat gcctctggct ctgtattgcc gtggccttaa gctttacggt ggaaatcgtg
    13021 cggcgcttcg taagtagcgg agttgatctt cccagtacga tagcgcaagg cttgcttgcg
    13081 ctacttgtcg gagggacact tatacagttc gccaggcagt tggttgaggg aatttcccgc
    13141 cagggtggaa agaagcttca attgaaatgg agaacgcagt taattctagc cgtagtcttg
    13201 gtaactgtgg cggtggttct tgaattgtcg cgcgctaaag tagttgccca ttacagtaac
    13261 atgggcgtgg atcagctcaa tgccggacag ctggctggag ccatccagaa tttccagcgt
    13321 ggcatcagtt tgaaacctaa tgacgcgatt gcgcatttca acttagctag cgcgtatgag
    13381 agcgtgctgg attatgataa ggccgaagct gaatacagag ctgccatcca gtggaataaa
    13441 caactctccc cagcctatag caagctagcc cgcctataca tactgcgccg caacgaccct
    13501 ggcgcggcac tgatgctgtt gagagcgggt ctcagggaaa ccgatgccca aagcttggga
    13561 gaaggaccca ctgatgataa ctggaaacga at
//
LOCUS       DASWNS010000395         4842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_93717, whole genome shotgun sequence.
ACCESSION   DASWNS010000395 DASWNS010000000
VERSION     DASWNS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93717"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..3714)
                     /locus_tag="VGB76_13790"
     CDS             complement(328..3714)
                     /locus_tag="VGB76_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEY0100016.1"
                     /translation="MPDTFKYDVFLSYSSKDKAVVRPIAERLRADGMRVWFDEWEIKL
                     WHSKAAREKRIEEGLEHSRALVLCLSANAFGSDWTKLEASTLRFRDPHNKERRFIPLR
                     LDNFPIKDSLTQFLHINWLPDDRQQAYVKLLEACRPPARQAVVGAEAMSGQFAEKFIQ
                     LDYKANIHVYAFSPDGKRALTGARDNTVRLWDMATGHCLRMFEGHALNLVYGVAWRAD
                     QRRALSSGLDDTVRLWDVETGHCLRVFEGHKASVFDVVWSVDQYRALSASHDMTVRLW
                     DVKTGHCLRVLEGHSNRVISVAWCDDGRHALSGGFDYTMRLWDVETGKCLRVLDGHTS
                     PVQSVKWSSDQRHALSGGSDHTVRLWDVKTGKCLRVLDGHTAEVTSVAWSTDQRHALS
                     GSDDKTVRLWDMETGECLRVLEGHTRGVRSMAWSVDQRRAFSGDAKGGISVWDLSEFV
                     SEAGVSKVAVPALRTVAEQVQYTNAKVLLVGESGAGKTGLSKVLAGEEWEPSDSTVGA
                     WATQWKLPVSSDGGVEREIWLWDFGGQADQRLIHQLYMDETALVVLVFDGQKEDLFET
                     LGQWDRDLTRASRNPFAKLLVAGRVDAGGLRVSRTQIEAFKNERGYLQLLETSAKTNH
                     NCEQLQQAILKAIQWENIPWRTSPLLFKRLKDEIIHLKDEGRVLLRFNELRETLQLRL
                     SGETGRFTDEELKAVVSLLAGPGVVWELKFGSWVLLQPERINAYAQAVIQTMREDRDE
                     RGCLPEERVLNGDLKYHSSIERLGDDEERFVLLAMHQTLVERGLCLREHTDQGALLIF
                     PSYYRRERPELVGHPAVLVSYRFSGFLDDIYATLVVRLHHTESFEADELWRYAADFKS
                     LSQQRRLGVKLTRRGEGAGELEVYFDPDIPVAEKMIFSRYVHEHLLKKGTEVVRLRHY
                     VCPHCKTPVRDRETAMERLTENGKKASILCVKCEKRVPLWDDLEERFAAPEVHQRVLE
                     LDEQSQIKLDNADKERTLVGEVISTVSLAGQICRELTVSDNGIDMEIEFKSNAGEATG
                     KKVYLQLKSGDSYLRKRKRDGAEIFTIPDERHARYWMEQAFPVMLVIRNSEGEVRWME
                     VREWLKSASDNGRKPVKQIVFEGERLDVMSVRRWRDRVLR"
     gene            complement(3859..>4842)
                     /locus_tag="VGB76_13795"
     CDS             complement(3859..>4842)
                     /locus_tag="VGB76_13795"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100017.1"
                     /translation="CPYFGRCGGCDFQQLTYEAQLEAKVEIIRDCLRRIARLEPPAEI
                     AITPSPAVWHYRSRARWQHDPVRQFLGYYELASHRVCDVSECPVVLPEVQAKLTYLRA
                     TLNAGASEETEEFQAVAGDDGVSLLPPLEAADAGERSRLLAGERYRFDADCFFQINHA
                     LLEPLLNAALKNTNDTTRGGTAVDLYCGVGLFTLPLARRFTRVIGVEGNASATAFARL
                     NLADAKLPNAEIETAHVGDWLTQHAPSLTPVDFLLLDPPRAGADPETLTGILTLRPAR
                     IAYVSCDPATLARDLRTLIAAGYTLDSLAAFDMFPQTHHVETVAHLARAIL"
BASE COUNT          919 a         1484 c         1410 g         1029 t
ORIGIN      
        1 taatcagtcc tttcggcgtg ggggttttag aactgctgta ggcgttaagt gcggcgcggc
       61 ggtggtcgct cacacgcgac cgtcccgcca gataaacaca gtgggcgcgt attatagtcg
      121 gttagatcag aaggtcaatg attttcaatg cgacgcaacc gggcggcaac tttgctcttc
      181 attcagtcca actataaaag tcgctgagaa ccggctaccc tcattattga ttgagacgga
      241 gtttcctacg atgaacgtct gccactgtta tcgtgcgcgc ggcgatggcg aataattaca
      301 aagcccgtcg aacctctccg gcaaaagcta acgcaagacc ctgtcacgcc agcggcgcac
      361 gctcatcacg tcgaggcgtt ccccttcaaa gacgatctgc ttcacgggtt tcctgccgtt
      421 gtcgctggcg ctcttcagcc actcgcgcac ttccatccag cgcacctcgc cctccgagtt
      481 tcggatcacc agcatgactg gaaacgcctg ctccatccag tagcgtgcgt ggcgttcgtc
      541 cgggatggtg aagatttccg cgccgtcgcg cttgcgcttc ctgagatatg aatcgcccga
      601 cttgagttgc aggtagacct tcttgccggt cgcctcgccc gcattcgact tgaactcgat
      661 ctccatatct atgccgttgt cgctgacggt gagttcccgg cagatttgac ccgcgagtga
      721 gacggtagag atcacctcgc cgaccagcgt tcgttccttg tccgcgttat cgagtttgat
      781 ctgcgactgt tcgtccaact ccagcacgcg ctggtgaact tcgggcgcgg cgaagcgttc
      841 ttcaaggtcg tcccacaggg gcacgcgctt ttcgcacttc acgcatagga tgctcgcctt
      901 ctttccgttc tccgtcagac gctccatcgc cgtctctcga tctcgcaccg gcgtcttgca
      961 atgcgggcag acataatggc gcaggcgcac cacctcggtt ccctttttca gcaggtgctc
     1021 gtgaacataa cggctgaaga tcatcttctc cgccaccgga atgtcggggt cgaaatatac
     1081 ttccaactcg cccgcgccct ccccccggcg cgtgagtttc acgcccaagc gtcgttgttg
     1141 cgagagcgac ttgaaatcag cagcgtaacg ccacagttca tccgcttcga aggattccgt
     1201 gtgatgcagg cgcacgacca gcgtggcgta gatgtcgtcg aggaaaccgc tgaagcgata
     1261 gctcaccagc accgccggat gcccgaccaa ctccgggcgt tcgcgccggt aatagctggg
     1321 aaagatcagc agcgcgccct gatccgtgtg ttcgcgcagg cagaggccgc gctccacgag
     1381 cgtctggtgc attgccagca ggacgaaccg ttcctcgtcg tcgccgagac gttcgatgga
     1441 agagtgatac ttgaggtcgc catttaagac gcgctcctcc ggcaggcagc cgcgttcgtc
     1501 tctatcctcg cgcatcgtct ggatcacggc ctgcgcatag gcgttgatgc gctccggctg
     1561 gagcagtacc caactgccga acttcaactc ccacaccacg ccgggaccag ccagcagact
     1621 caccaccgcc ttcaactctt cgtcggtgaa acgcccggtt tcaccggaca gccgcagttg
     1681 cagcgtctcg cgcagttcgt tgaaccgcag cagcacccgt ccctcgtcct tgagatgaat
     1741 aatctcatcc ttgagccgct tgaagagcag cggcgaggtg cgccagggaa tgttttccca
     1801 ctgaatggcc ttgaggatgg cctgctggag ttgctcgcag ttgtggttgg tcttcgcgct
     1861 ggtttccagc agttgcaggt agccgcgctc gttcttaaaa gcctctatct gcgtccggct
     1921 gacgcgcaag ccccctgcgt caacgcgccc agcgaccagc aacttggcga acgggttgcg
     1981 tgaagcgcgc gtcagatcac gatcccactg cccaagcgtc tcaaacaggt cttccttctg
     2041 accatcgaag accaatacca ctaacgccgt ctcgtccatg taaagctggt gaatcaacct
     2101 ctggtccgcc tgcccgccaa aatcccacaa ccaaatttcc cgctccaccc caccatccga
     2161 agacaccggc aacttccact gcgtcgccca cgcccccacc gtcgaatcac tcggctccca
     2221 ctcttccccc gccaacacct tcgacaaccc cgtcttcccc gccccgctct ccccgacaag
     2281 caggactttg gcattcgtgt attgaacttg ttctgcgacg gtacgcaatg ctggcacagc
     2341 gactttcgac actcccgcct cgctgacaaa ttccgacaga tcccagacac taatgccgcc
     2401 cttagcgtcg ccggagaagg cgcggcgttg atcgacgctc cacgccatgc tcctgacccc
     2461 acgcgtatgg ccttccagca cgcgtaaaca ttcccccgtc tccatgtccc acagtcgcac
     2521 cgtcttgtca tcgctaccgg agagggcatg ccgctgatcg gtgctccatg ccacacttgt
     2581 aacttcggca gtgtgaccat cgagcacgcg taaacatttc cccgtcttca catcccatag
     2641 ccgtactgta tgatcagatc cgccggagag ggcgtgtcgt tgatcgctgc tccacttcac
     2701 gctctggacg ggagatgtgt gaccatcgag cacgcgtaaa catttccccg tctccacatc
     2761 ccacagccgc attgtataat caaatccacc ggagagagcg tgtcgtccat catcgcacca
     2821 cgccacgcta ataactcggt tcgagtggcc ttcaagcacg cgcaaacaat gccccgtctt
     2881 cacgtcccat agccgcaccg tcatgtcgtg agaggcggag agggcgcggt attgatcaac
     2941 gctccatacc acgtcaaaaa cagatgcctt gtggccttcg aacacgcgca ggcaatgtcc
     3001 agtctctaca tcccacagcc gtacagtatc gtctagtccg ctggagaggg cgcgacgttg
     3061 atcggcacgc cacgccacgc catagacaag atttagcgcg tggccttcaa acatgcgtag
     3121 gcagtgcccc gttgccatgt cccacaaccg cacggtattg tccctcgcac cggtgagagc
     3181 gcgcttacca tccggactga atgcgtagac atggatgttc gccttgtagt cgagttggat
     3241 aaacttctct gcaaactgcc cgctcatggc ctctgctccc accaccgcct gccgcgcggg
     3301 agggcggcag gcttcgagga gtttcacgta tgcctgctgg cggtcatccg gcagccagtt
     3361 gatatgaagg aattgcgtta gggagtcttt gatggggaag ttgtcgaggc ggagagggat
     3421 gaagcgtcgc tctttgttgt gggggtcgcg gaagcgtaag gtgctggcct ccaattttgt
     3481 ccagtcggag ccgaaggcgt ttgccgacag gcagagcacg agcgcgcggg agtgttccag
     3541 cccttcctca atcctctttt cgcgtgcggc ctttgagtgc cagagtttga tctcccactc
     3601 gtcgaaccac acgcgcatgc cgtccgcgcg cagccgttcg gcgatggggc gcacgaccgc
     3661 tttgtccttt gagctgtaac tgaggaacac gtcgtatttg aaagtgtcag gcatgggttc
     3721 acctcaataa ttctttagtg cggagatagt atcatcccat aaaaggctct tcgtggaatt
     3781 agtatatgcg gcttcttaat cagcgtttca ttttctgatt gtgcgttcca aggccggatt
     3841 ctcgaagact tcggttcttt acaggattgc ccgcgcgagg tgtgcgactg tttcgacgtg
     3901 gtgggtttgc gggaacatgt cgaaggcggc gagggagtcg agggtgtagc ctgcggcgat
     3961 gagggtgcgt aggtcgcgtg cgagggtggc ggggtcgcag gagacgtagg cgatgcgcgc
     4021 gggacggagg gtgaggatgc ctgtgagagt ttcggggtct gcgcctgcgc gcggggggtc
     4081 gaggaggagg aaatcgacgg gcgttaaact tggggcatgc tgtgtgagcc agtcacctac
     4141 gtgcgccgtc tcgatctcgg cgttcgggag tttggcatcg gcgaggttga ggcgtgcgaa
     4201 ggcggtggcc gacgcgttgc cttcgacgcc gatgacgcgt gtgaagcggc gcgcgagcgg
     4261 gagcgtgaac aggcctacgc cgcagtagag atcaactgcc gtcccgccgc gcgtcgtatc
     4321 atttgtgttt ttgagggcgg cgttgaggag cggttcgagc agcgcatggt tgatctgaaa
     4381 aaagcagtct gcgtcgaagc gataacgctc gcccgcgagc aggcgggagc gttcgcctgc
     4441 gtccgctgct tcgagcggcg gcaggagcga gacgccgtcg tcgcccgcga cggcttgaaa
     4501 ttcttccgtc tcttcagacg cgcctgcgtt gagcgtcgcg cgcaggtacg tgagtttggc
     4561 ttgcacctcc ggcaagacga cgggacattc gctaacgtcg cagacgcgat gagaggcgag
     4621 ttcgtagtag ccgaggaatt gacgcacggg gtcgtgttgc cagcgtgcgc gcgagcggta
     4681 atgccagacg gcgggcgacg gcgtgatggc gatctctgcg ggaggttcga gccgcgcgat
     4741 gcgacgcagg cagtcgcgta tgatctcgac cttggcttcg agttgtgcct cgtaagtcaa
     4801 ctgctggaag tcgcagccgc cgcagcgacc gaagtagggg ca
//
LOCUS       DASWNS010000396        19055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_352663, whole genome shotgun sequence.
ACCESSION   DASWNS010000396 DASWNS010000000
VERSION     DASWNS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19055
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352663"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VGB76_13800"
     CDS             <1..504
                     /locus_tag="VGB76_13800"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0100018.1"
                     /translation="SKSLEVGHIFKLGTKYSVSMNAKVLDQNGKEVPVVMGSYGIGIE
                     RIMAAAIELHHDADGIIWPRAIAPFDAVVTVTNIKQQELMDAGERLYKELRAAGLEVL
                     LDDRDERAGVKFKDADLIGIPYRITVGKKITEGTVELFDRATKQSTDVKLDDVAARLQ
                     ELALTGL"
     gene            complement(627..1619)
                     /locus_tag="VGB76_13805"
     CDS             complement(627..1619)
                     /locus_tag="VGB76_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100019.1"
                     /translation="MTNKRIRILAYATLIVLSCCTGAHAQTAFQDASGESSIFIQDRG
                     GFSRINVGDKSISLGYLLDSGDDSPFFGFELSGKRAGDFASIFSGSVPGNEAKINVSF
                     GKRFLYAKPPTPANAGQISTSRITLDWLTFQAGYKRSRYKLFTADPTFANQVRKQNFD
                     GYTATLGYNALINLTRSDDKTDASTDAADSAAGDISTPSSMIVGVSLGVQRRNNADDL
                     DTVDVEDEVFSATSGTTERRVLSKQQALSGEYKVSTAVPLNADVVIFPGRFKGRIALD
                     IFARSNLGEGGRNFVPGVGLFFTKKGAPTKVLGGLSISARDGKAQVGLVAGFHF"
     gene            complement(1631..1954)
                     /locus_tag="VGB76_13810"
     CDS             complement(1631..1954)
                     /locus_tag="VGB76_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100020.1"
                     /translation="MSKHRVQVVVEGALLSVFVFLEGTQLKLQSNDSGTYQGAKTLEV
                     KDPLDLIFHAFGVPPTEWKISLTVDGANKPLFEHEGMIGKHNQSVLQQAIPLPQTAAA
                     KGKNK"
     gene            2819..4129
                     /locus_tag="VGB76_13815"
     CDS             2819..4129
                     /locus_tag="VGB76_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0100021.1"
                     /translation="MLSNSTTTPILETDAFTKHLGMFTHPKGAHLTERTKPLGDWLQQ
                     RIEFGTWPYSRSLETSPATNARLKSSNGIRVEGVNFGSQDYLGLASHHAIHDAAIRAL
                     SDYGPHSAASAILQGNSELSRSLEAALAEHVQAEHVLLFPTGWAAGFGTIVGLVRPND
                     HIVLDQNSHACLMQGARAATDKHTFFRHNDVADARRKLSKIRAKDTENAILVVTEGIF
                     SMDSDSPDIFALQETCREFNAVLLVDVAHDLGSSGAQGTGQIGLQGMLGKVDLVMGAF
                     SKTFASNGGFLATHSASVRHFVSIYGGSHAFSNALSPIQAGVVREALRIVRSTEGESL
                     RARSLRNIRQLRSAFEAEGLECYGQPSPIVPVGVGDERLAKLTARALEENGLIANMVE
                     YPAVARGRARFRFQVMATHTPEQIDKGVSIFRRSLAEARLQLAG"
     gene            complement(4675..6159)
                     /locus_tag="VGB76_13820"
     CDS             complement(4675..6159)
                     /locus_tag="VGB76_13820"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100022.1"
                     /translation="MSKKRESFALIVHHSLLITFVIALTTAGCGARRTPDLERIFAPA
                     RARKDKLPVIVIPGILGSQLMNRRTREVVWPSAFRSSTDGLSLPTTPDLAANRDELVA
                     ERIVVTAKLARLAPDIYVYYELLNALRRFGDYRDGDWDNPPADGDRDTFYVFPYDWRR
                     DNVESAQLLFRRVEELKLKLGRPDLRFNIVAHSMGGLVARYAAMYGDADLPAGDAPGA
                     PQPTWAGARHINKIFMFGTPNEGTMEAFATLLEGYSINEGLRRRVRLFNKLSREDAVT
                     IPSIFQLLPHPETVRFLDQNLAPLEIDFYDPEVWRKYQWSPVNNAEFRERYERGRVRD
                     EDGAPHRATLAELDAYFATALRRARRFHAALDAHASDASPVALYVFGGDCEETLNSPV
                     ILRDEKRGLWRTLTEPRELRDTSGRKLKRREVTRAMYEPGDGRVTRRSLLGERPGAPR
                     RSPFFNTPLPIAYGVFACDLHSDLQSNKTLQDNALTLLINEVMN"
     gene            complement(6166..6939)
                     /gene="larB"
                     /locus_tag="VGB76_13825"
     CDS             complement(6166..6939)
                     /gene="larB"
                     /locus_tag="VGB76_13825"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEY0100023.1"
                     /translation="MDLREIEQLLSAHRTGELATAEAAQRLANLHFEDIEHTRIDHAR
                     AARQGFPEVVFGAGKSRTQICEIVERLVARAPNVLITRTDADTYGEVRNIATDAEWHE
                     SARMIRIRRDRTELGTGEIGIVTAGTSDIPVAEEAALTAEAMGNRVARIWDAGVAGIH
                     RLLAERERLRRFRVVVVAAGMEGALPSVVGGLVGVPVVAVPTSIGYGASFGGVAALLG
                     MLNSCASNVTVVNIDNGFGAGFVASLINRKWNKEVMSDE"
     gene            7159..7509
                     /locus_tag="VGB76_13830"
     CDS             7159..7509
                     /locus_tag="VGB76_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100024.1"
                     /translation="MRSLCSPSASRISRWRDFFLLLKGTGVAGIIIAIYTFPLGLFIV
                     LGHNVWVADVPLIVTLCGWGMTLKSASYALVPGQAERMIPSGADAHRKYFAGGFVALA
                     IGALLLYHSFFRAP"
     gene            7547..8191
                     /locus_tag="VGB76_13835"
     CDS             7547..8191
                     /locus_tag="VGB76_13835"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HEY0100025.1"
                     /translation="MELTLAITGASGATYAHRTLIHLASSGVVERINLIMSAAAQTVA
                     RVELGANLQGGDARTINEWIGLAPDSKLVRLHRLDNVAATPASGSHPQAGMLIVPCSM
                     GTLGAIASGAGTNLIHRAADVTLKEGRKLVIVPRESPYNAIHLENMLRLARAGARILP
                     ASPGFYHRPQSIEELVDHLVFRILDQFGVPHTQRTQWQGDPTATTTAPVAIEGD"
     gene            8413..9306
                     /locus_tag="VGB76_13840"
     CDS             8413..9306
                     /locus_tag="VGB76_13840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEY0100026.1"
                     /translation="MSSQATNTTTAAGNLRTTLEMIKIEHTLFALPFALLGAVLAARG
                     VPSVWQLLWITVAMVGARSAAMAFNRIADHRYDAANPRTSGRAIPSGALSLKFAWAFT
                     LASASLFFVAAAMLNRLTLLLAPVALASVLLYSYTKRFTSLSHLVLGWCLAIAPTGAW
                     IAVRGAFDSPVPLLLSLVVMLWTAGFDVLYACQDYDFDRAAGLRSIPARVGIGRALWI
                     SRLLHAAAFAALVALYLTAKPGAFALAGLAATGALLVYQHSLVRADDLSRLNAAFFTT
                     NAFVSVILLLTFGGAVFFARL"
     gene            9351..9962
                     /locus_tag="VGB76_13845"
     CDS             9351..9962
                     /locus_tag="VGB76_13845"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0100027.1"
                     /translation="MSTNTSIPTEREPIVLHFSPRLKQISEEEFYEFCQLNRDLRLEL
                     TSEGDLIIMPPTGGKSGLRNATLTAELKNWGKQDGTGLVFDSSTVFALPNGAKRSPDA
                     SWVSKDRWSALSEDEQEQFPPLCPDFVVELRSRTDALKNLRQKMEEYVANGAQLGWLI
                     DPSERKVYVYRPSAPAEELDNPQTISGEPLLRGLSLNLQEIWD"
     gene            9995..11113
                     /gene="mqnE"
                     /locus_tag="VGB76_13850"
     CDS             9995..11113
                     /gene="mqnE"
                     /locus_tag="VGB76_13850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEY0100028.1"
                     /translation="MTVMNFSHDPKLKEIAAKVEAGERLSFTDGMDLYATDDLAALAK
                     LADTVRRRLHGRRTYYNVNRHFNYTNVCYADCKFCGFYRTPRQPDAYTHNVEEAVAVA
                     GKAVAEGATELHIVGGLNTKLPFKYYTDLLANLKRAYPRLHLKAFTMVELDHMAGFYK
                     MSDEEVIARLKEAGMDSCPGGGAEIFREPTRSRICAHKTDAGRWLALAGKVHDAGLKT
                     NATMLYGHIESVEDRVDHFIQLREQQDASGGFQCFIPLAFYPEGTKLSACPGPSGVDS
                     LKTIAVSRLMLDNFPHIKAYWVMLGKRLAQVALHYGANDLDGTITEGGELTESYSVES
                     NNEVRMNKNEIAALIREAGFEAVERDTLYNPIVEMTAA"
     gene            11181..11978
                     /locus_tag="VGB76_13855"
     CDS             11181..11978
                     /locus_tag="VGB76_13855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0100029.1"
                     /translation="MSVIKMEVAMTEIEPTKRFTERVADYVAYRPKYPAAVLEFMRAE
                     LNLRPSSIIADVGSGPGILTEMFLRNGNTVFAVEPNEAMRAAAENLLGAFPNFKSVDG
                     MAEATTLDARSVDFVTAAQAFHWFDAGRALAEFARILKDGGWVALLWNMRRTDSTPFL
                     RELERILRAYGTDYERVAADNPGQELMERIFPRGFGTKRFRHEQVFDYAGLRGRWLSA
                     SYVPLAGHPNHEPMFDALRRAFDEHNTDGTVVIEYDTVLYYSQLSKV"
     gene            12058..12906
                     /locus_tag="VGB76_13860"
     CDS             12058..12906
                     /locus_tag="VGB76_13860"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEY0100030.1"
                     /translation="MTTDTRTQTITVAHSPDSDDAFMFYGLATNKLDTGSLRFTHVLE
                     DIQSLNEKAMQGVYDVTAISFHAYAYISDKYALLPHGASIGDNYGPIVVSREKFKPEE
                     LSSLKVAVPGKLTSAFLALSIFCPEFEYEIVPFDKIIEAVQEGRADAGLLIHEGQLFY
                     QNMGLHKVLDLGEWWHERTGLPLPMGGNVIRRDLGAEVISQVSKCLHESIRYSLDNRA
                     DALEYAMQFARDMEPELADRFVGMWVNELTLDYTERGRTAVQRLLDEGAERGIIPRRV
                     DVEFVQ"
     gene            13095..13949
                     /locus_tag="VGB76_13865"
     CDS             13095..13949
                     /locus_tag="VGB76_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100031.1"
                     /translation="MKNSLSWQSRLRLFVLLLACAAFAPAAGAQQVSSASAPSASTAG
                     VDVERIIREFTAKEATFRQALNQYSFKREAVVQTIGMGGQISGEYRRISRFVFDDSGK
                     RYEKILLFPLPTLTEVELSGEDLEDLSGVQTFALEPSKINLYQLTYVGKEKIDELDCY
                     VFDVAPKVMPDPKKTKDRFFKGRIWIDDQDVQIVKVRGKGVPEKKNARYPTYETYREQ
                     IDGKFWFPTYTYADEELIFDSGQVVRLRLRITFNEFVRPSAKVRVIDEGQDIIPTPSP
                     TPQQQKKP"
     gene            complement(14073..14966)
                     /locus_tag="VGB76_13870"
     CDS             complement(14073..14966)
                     /locus_tag="VGB76_13870"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0100032.1"
                     /translation="MTDSLSEKLFDTESVCAGGESSTDASAPRTSKQDAMAGGEAAAA
                     GRLVAGGAGESSFAGRLYHWWILFVAAMLLLIFGPPVLLVAWLAGRREWVYPIALWGG
                     RQWLRLSGMRVRVRGLEQLDPRRTYIFTANHRSYLDTATLFCHLKRRIGLFAKKELLK
                     VPILGFGMGFVNIMAIDRSNRERALATVRAATERIRSGVSFGVFAEGTRALPGQLLPF
                     KKGAFYMAIETGVSIVPVAIKNTDALMGKGTGVARAGTIEMVILPPVETTGLSTDEDV
                     RRLAGTVRELVAEELRTEFRD"
     gene            14965..17484
                     /locus_tag="VGB76_13875"
     CDS             14965..17484
                     /locus_tag="VGB76_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY0100033.1"
                     /translation="MSRSVLRLALSTYLVALSLVVVLCGALFAPAPVRAQQTRRPASP
                     ASRSAATTTNAPPSPASVLGFTPGDDRTVADWRQITDYFTRLDAASERVEVRTLGQTT
                     LGRPLVVAFISAPENIRNLTKFQDLQRRLSDPRLASTEAARDELTREGKTVVVISCSI
                     HSTEIVASQMSMQLAYELASAADAATREILQNTILLLIPAANPDGVDIVANWYRKTLG
                     TPYEGTEPPELYHHYAGHDNNRDWFMLNLKETQLLTRLFWREWFPQIVYDVHQQGANG
                     SRFFVPPFFDPPNPHIPPLLLREVGLIGHKIAADLQAAGQQGVITNALYDTWWHGGFR
                     TAPYYHNSVGILTEAASARLMSPVEVTREQLTRSTARGMPSALQSATNFPDPWPGGTW
                     RPRDIMRMELTASRAVLSMAAKYRERYLHNFYELGRRGVEGRNTVGDAEIGYVIPAGQ
                     GKDEAVAKLIGALVEQGVEVQRLDKELHLMMLGNRGAVYNDLVQNPPVMPAGSYLIFL
                     AQPYRANIKALFQKQIYPDRRTAGGEAERPYDVAGWTLPMQMGVQALPIHSIREAASE
                     RRLTLIRDANEVRRALNLPAQQDTRSPLTNPVARPSRLALYKSWTGSMDEGWTRYVFD
                     TFNVPFQTLLDKEVRAGNLRARYDVIVLPSMRLREIVEGRSRETAPPEFAGGITEAGV
                     ENLRRFVEDGGTLVCFDAAAELAVKRFKLPLRNVLEGVRSSEFYGPGSILRLEVDTAH
                     PLARGFGKQVDAYFINSSAYELTADAEKRVRVVARYAERDVLRSGWLLGEKLIAGKIA
                     LAEVNLGKGRVVLFAFRPQHRAQTWGTFPFIWNAIELRDEG"
     gene            17649..19013
                     /locus_tag="VGB76_13880"
     CDS             17649..19013
                     /locus_tag="VGB76_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0100034.1"
                     /translation="MRKFAALLALLSLLATSAASINAQTRPRRVGQTTGRANPPDSSS
                     TTAPRSDAENDRGARRPPTLGGAVGSNDTASARTSNTTDPTEVDENEVVRVNTSLVTI
                     PVSVMDRAGKYVPSIRQQEFRIWEEGVEQEIAYFATVEKPFTVALVIDTSGSTKFRLR
                     EIQDAAIAFVNQLRPEDRVLVVSFDDEVRVLCEATSDRARLHDAILRVYPGEGTKLYD
                     AVDFIINQRFNRIEGRKAMLLFTDGVDTSSRRASYESNARDAEELDALIYPVQYDTFA
                     GGNSPSGNGGGVWGGGNRRRRGGMADILSDILIGGNVRIGGGSGGGRGGSGGGGGGNS
                     CTGCTREEYARGNAYLNDLARLSGARLYRADTTYDIAGAFSLVAEELRRQYSLGYYPK
                     QQGQPGQRRRIKVRVMRPDLAVNARDSYIYNPSGANNNTTTAQDSNQQRSQPVLRRSP
                     LAER"
BASE COUNT         3660 a         6044 c         5759 g         3592 t
ORIGIN      
        1 tcgaagtcgc tcgaagtggg tcacatcttc aagctcggca cgaagtattc cgtctcgatg
       61 aatgcgaagg tgctcgacca aaacggcaag gaggtgcccg tcgtgatggg cagctacggc
      121 atcggcatcg agcgcatcat ggccgccgcc atcgaactcc atcacgatgc ggacggcatc
      181 atctggccgc gcgccatcgc gcccttcgac gccgtggtca ccgtcacaaa catcaagcag
      241 caggagttga tggacgcggg cgagcgtctc tacaaggaac tgcgcgccgc cggactcgaa
      301 gttctgcttg acgaccgcga cgagcgcgcg ggcgtcaagt ttaaagacgc agacctgatc
      361 ggcatccctt accgcatcac cgtcggcaag aagatcacgg aaggcacggt cgaactcttc
      421 gaccgcgcaa cgaaacagtc aaccgacgta aaactcgacg acgtggccgc acgcctgcaa
      481 gaactcgcgc tcacggggct gtaagtcgca aggccgtgca tcgcggcgaa gggcgacacg
      541 gaaccgtgag ttccgtgtcg cccttcgtcc gtttaagagg cgagagcctg tgacgtttcc
      601 tgagcagcgg gagcatgcaa ccttctttag aagtggaagc ccgcgacgag gccgacctgt
      661 gcttttccgt cgcgcgcgga gatgctcagg ccgcccagca ctttcgtcgg cgcgcctttt
      721 ttggtgaaga acaggccgac gccgggcacg aaatttcgcc cgccctcgcc gagattggag
      781 cgcgcgaaaa tgtcgagcgc gatgcggcct ttgaagcgac cggggaatat caccacgtcc
      841 gcattgagcg ggacggctgt ggacaccttg tactcgccgc tcaacgcctg ctgcttggag
      901 agaacccgtc gctccgtcgt cccgctcgtc gcagagaaga cctcatcctc aacgtcaacc
      961 gtgtccagat cgtctgcgtt attgcgtcgc tgcactccga gcgagacgcc gacgatcatg
     1021 gaggacggag tcgagatgtc gcccgcagcg gagtccgccg cgtccgtgga ggcatccgtc
     1081 ttgtcgtcgc tgcgcgttag attgatgagg gcgttatatc ccagggtcgc cgtgtagccg
     1141 tcgaagttct gtttcctgac ctggttggcg aaagtggggt cggcggtgaa gagtttgtag
     1201 cgcgaacgct tgtaaccggc ctgaaaggtg agccagtcga gcgtgatgcg gctggtggaa
     1261 atttgcccgg cgttggctgg cgtcgggggt ttagcgtaga gaaaccgctt cccgaaggag
     1321 acgttgatct tcgcttcgtt tcccggcacg ctgccgctga agatagaggc gaaatcgccc
     1381 gcgcgcttgc cgctcagttc gaagccgaag aagggactgt catccccgct gtcgagcagg
     1441 tagccgaggc tgatgctctt gtcgcccacg ttgatgcgcg agaagccgcc ccggtcttga
     1501 atgaaaatac tgctctcgcc cgaggcgtcc tgaaaggccg tctgcgcgtg cgcgccggtg
     1561 cagcacgaca acacgatgag cgttgcgtat gccaatattc gtatgcgttt gttcgtcatc
     1621 actctgctcc ttacttattt ttacccttag ccgccgccgt ctgcggaagg gggatggcct
     1681 gttgcagcac gctctggttg tgcttgccga tcatgccctc gtgctcgaac agaggtttgt
     1741 tcgctccgtc tacagtgagg gagattttcc actccgtcgg cggcacgccg aaggcatgaa
     1801 agatgaggtc gagggggtct ttaacctcaa gcgtttttgc gccctgataa gtgccgctgt
     1861 cgttgctctg caacttgagc tgcgtccctt ccagaaaaac gaagaccgat aagagcgcgc
     1921 cttcgaccac tacttgaact cgatgtttac tcatggaacg atgaacctcc tgagagatag
     1981 ttgtgggatt gactgataag tctcgcaagg tgtttcctgc cgactcgcgc gaacgtccgg
     2041 cgcggcgtcc gccgtcacgg gcacgcacgc cgtctgcggc taacgcgcca tccaaacgct
     2101 cgctttcacg aggctgttaa tagctatcgg cgtgccgtgt ccgcagcaca acggattggc
     2161 atcgtttcgc agggacgtaa atttatgagg cagggcggat taacttccgg cagggagcgt
     2221 gggaggtgag gcggggcgtc tgcgcacgcg tgcgtcgaac ctatcagtgt tgatagtcgg
     2281 gctatcaaca ctgataggtg gcgggcgcga ttgacggcgc aagagggtct taaaggtagc
     2341 ccggcctgtg ctccggcagg gtcgtttcca gagtcgtgaa gggtgggaaa gttgcgttcc
     2401 gtttagtttc cgaagcgtcg tgcagcgcat ctgaaatttc gtttgacatc ggcgagtgat
     2461 ccgtttactc tgcacgcgct tcaaatggaa cgttagagct tggccgctct gttcgacgtt
     2521 ataaaaatca accgacaatt gtagaaacga tgtgattcat taccttcgcg tgtaatcccg
     2581 tctttttcaa ggccaacgtg cgcccctatt ttctcaggcg cgatcaaact tccgacaata
     2641 aaagcttcac gctcaaacgc tcgttctctt tctcgcgtga gccttgaggt ggtcagaagt
     2701 atgtccggcg cgtgctgccg ccagtgttgc cgccctcttc gtgtctctca aagctcgaac
     2761 ggcaacgaag cgatccgaat tctctcatca accctcaaca cgaaaggaac tacgccccat
     2821 gctgtcaaat tccaccacga ccccgatcct cgaaaccgac gccttcacca aacacctcgg
     2881 catgttcaca caccctaaag gcgcgcacct caccgaacgc accaaaccgc tcggcgactg
     2941 gctccaacag cggatcgaat tcggcacgtg gccttacagc cgttcgctcg aaacttcgcc
     3001 cgccaccaac gcccgcctta aaagctcgaa cggcatcagg gtggaaggcg tcaacttcgg
     3061 ctcgcaggat tatctcggcc tcgcctcgca tcacgcgatt cacgacgcag ccatccgcgc
     3121 gctctccgac tacggaccac acagcgcggc ctccgcgatc ctgcaaggca actccgaatt
     3181 gtcgcgctcg ctcgaagccg cgctcgccga gcacgtgcag gccgaacacg tcctcctctt
     3241 cccgaccggc tgggctgccg ggttcggcac catcgtcgga ctcgtgcgcc ccaacgacca
     3301 catcgtcctc gatcaaaact cgcacgcctg tctcatgcag ggcgcacgcg ccgccaccga
     3361 caagcacacc ttctttcgtc ataacgacgt ggcggatgcg cgccgcaagc tctcgaagat
     3421 cagggcgaag gacacggaga acgccatcct cgtcgttacc gaaggcatct tctcgatgga
     3481 ctccgacagc cccgacatct tcgccttgca ggaaacctgc cgcgagttca acgccgtgct
     3541 gctcgtggat gtggcgcacg acctcggctc gtccggcgcg cagggcacag gccagatcgg
     3601 cttgcagggg atgctcggca aggtcgatct cgtgatgggc gcgttctcca agacgttcgc
     3661 ctcaaacggc ggcttcctcg ccacgcattc ggcctccgtg cgccacttcg tcagcatcta
     3721 cggcggctcg cacgccttct ccaacgcgct ctcgccgatt caggccgggg tggtgcgcga
     3781 agccctgcgc atcgtccgct cgacggaggg cgagagcctg cgtgctcgct ccctgcgcaa
     3841 catacggcaa cttcgctcgg ccttcgaggc cgaaggcttg gagtgctacg ggcagccttc
     3901 gcccatcgtg cccgtcggcg tcggcgacga aaggctggcg aagttgaccg cccgcgcgct
     3961 cgaagagaac ggactcatcg ccaacatggt ggagtatccg gcggtcgcgc gcgggcgcgc
     4021 acgcttccgc tttcaggtca tggcgacgca tacgcccgaa cagatcgaca agggcgtctc
     4081 gatcttccgc cgctcgctcg ccgaagcgcg gcttcaactc gccggatagg agcggctcgg
     4141 acacgcgcac ggtcgcgcgc ctttattgcg gagcgatctc gatcaaggtt acttccggcc
     4201 tcgcccagaa gcgcacgcgc ggggcgaggc cgccctccat gccgatgccg cgattgacgt
     4261 agaggtgcgt ctcgtccaca cggtagaggc cggacgcgtg ccgcgcggca gtttcgtcct
     4321 ctcgacgcgt acgcgcgtga cggcgagcca gtagggttcg atgaagtagc cgtaggccat
     4381 gcacaagacg ccgatcacgg cgagcgtcag gacgatgcgt tggagccaga gttgcgtccc
     4441 ctccggcttg ctctcaagga cgcccgcgcg gcgcagcagg gcgcggatga gcgcggatga
     4501 gcgcgaaggc cgcgaacagg tagacggtgg cgacggtgcg gaggaagagc ggcagcatga
     4561 attcttccat agtcttaacc gttaaaccgc tcgtgcatta ttgtcccggc agaattgcgc
     4621 gacgcttcac gccgtcgcgc ccggatgcgt tccacacatt gcgcggcctc tctgctagtt
     4681 catcacttcg ttaatgagca gcgtgagcgc gttgtcctga agcgtcttgt tgctttggag
     4741 gtcgctgtgg agatcgcagg cgaagacgcc gtaggcgatg gggagcggcg tgttgaagaa
     4801 ggggctgcgg cgcggcgcac cgggacgttc gccgagcagc gagcgacgcg tgacgcgccc
     4861 gtcgcccggt tcgtacattg cgcgcgtcac ctctctgcgt ttgagctttc gccccgacgt
     4921 atcgcgcaac tcgcgcggct ccgtgagcgt gcgccagagg ccgcgttttt cgtcgcgcag
     4981 aatgacgggc gagtttaaag tctcttcgca atcgccgccg aagacgtaga gcgcgacggg
     5041 gcttgcgtcg cttgcgtgcg cgtcgagcgc ggcgtgaaag cggcgtgcgc ggcggagggc
     5101 ggtcgcgaag taggcgtcga gttcggcgag cgtggcgcga tggggcgcgc cgtcttcgtc
     5161 gcgcacgcgc ccgcgctcgt agcgttcccg aaactccgcg ttgttaacgg gcgaccattg
     5221 atacttgcgc cacacttcgg gatcgtaaaa atcaatttcg agcggcgcga gattttgatc
     5281 gaggaagcgg acggtttccg ggtgcggcag cagttggaag atggagggga tcgtgacggc
     5341 atcttcgcgc gagagtttgt tgaagagacg cacgcggcgg cgcagccctt cgttgatcga
     5401 atagccttcg agcagcgtgg cgaaggcttc catcgtacct tcgttgggcg tgccgaacat
     5461 gaagattttg ttgatgtggc gcgcgcccgc ccaggtcggt tgcggcgcgc cgggggcgtc
     5521 gcctgcgggc aagtccgcgt cgccgtacat ggctgcgtag cgcgcgacga ggccgcccat
     5581 cgaatgcgcg acgatgttga agcgcagatc ggggcgaccg agcttgagct tcaactcctc
     5641 gacgcggcga aagagcagtt gcgccgactc cacgttgtct ctccgccagt cgtaaggaaa
     5701 gacgtagaag gtgtcgcggt cgccgtcggc gggcgggttg tcccagtcgc cgtcgcggta
     5761 atcgccgaag cggcgcagcg cgttcaggag ttcgtaatag acgtagatgt cgggcgcgag
     5821 ccgcgcgagc ttggcggtca cgacgatgcg ctcggcgacg agttcgtcgc ggttcgcggc
     5881 gaggtcgggc gtcgtcggca ggctcaggcc gtcggtcgag gaacgaaacg cggaaggcca
     5941 gacgacttct cgcgtccgcc ggttcatgag ttgcgaacct agtatgccgg gaatgacgat
     6001 gacgggaagt ttatctttgc gcgcacgcgc gggagcgaag atgcgttcga ggtcgggcgt
     6061 gcgacgcgcg ccgcaacccg ccgtcgttaa cgcgataacg aaagtgataa gtaatgagtg
     6121 atgaacgatg agcgcaaagc tctctctctt tttactcatc actcatcact catcactcat
     6181 cacttcttta ttccatttac ggttgatgag gctggcaacg aagcccgcgc cgaagccgtt
     6241 gtctatgttg acgacggtga cgtttgaagc gcaggagttg agcatgccga ggagcgcggc
     6301 gacgccgccg aaggacgcgc cgtaaccgat gctcgtcggc acggcgacga ccggcacgcc
     6361 gacgaggccg ccgacgacgg aaggcagcgc gccctccatg cccgccgcca ccaccacgac
     6421 gcggaaacgg cgcaggcgtt cgcgctcggc gagcaggcga tgaatcccgg ccacgcccgc
     6481 gtcccagatg cgcgccacgc ggttgcccat cgcctccgcc gtcaacgccg cctcttccgc
     6541 cacgggaatg tcgctcgtgc ctgcggtgac gatgccgatc tcgcccgtgc cgagttccgt
     6601 gcggtcgcgc cggatacgga tcatgcgcgc cgactcgtgc cactcggcgt cggttgcgat
     6661 gttgcgcacc tcgccgtaag tgtccgcgtc cgtccgcgtg atgaggacgt tcggcgcacg
     6721 cgcgacgaga cgttccacga tctcgcagat ttgcgtgcgc gactttcccg ccccgaagac
     6781 gacttcggga aaaccctgtc gcgccgcacg cgcatggtca atgcgcgtgt gctcgatgtc
     6841 ttcgaagtga agattggcga gacgctgcgc cgcctctgcc gtcgccaact cgcccgtcct
     6901 gtgcgcggag agcaattgtt ctatttcgcg tagatccatc ttaatatgaa gctgcccact
     6961 gtttgacgtg cgcccattct agcatcgaac ccgcgcgcgc ccgcaatttg agcgcgcgct
     7021 acctctctcg ctttgcttcg cgcgactgaa acatagcgcg gcggcggcgc gtaagttttg
     7081 attcttcaag cccgccgcgc ggcagcgttc agacatccct gacgggaggc tccgtggaac
     7141 ttttcatcga acgttatttt gcgatcacta tgctcgccgt cggcctctcg catatcgcgc
     7201 tggcgcgact tcttcctgtt gctgaaaggg acgggcgtgg ccggaatcat catcgccatc
     7261 tacaccttcc cgctcggcct gttcatcgtc ctcggacata acgtctgggt ggcggatgtg
     7321 ccgctcatcg tcaccctctg cggctggggg atgacgctca agagcgcgag ttacgcactc
     7381 gttcccggcc aggccgagcg catgattccg tcgggcgcag atgcacatcg caagtacttc
     7441 gcgggaggct tcgtggcgtt agccatcggc gcgcttttgc tttaccattc atttttccgc
     7501 gccccgtaaa atccgcgaac ttcgcaaccg gcgcggcctg cgacttatgg aattgaccct
     7561 tgccatcacg ggagcatcgg gcgcgactta cgcgcaccgc acgctcatcc atctggcgtc
     7621 gagcggcgtg gtcgagcgca tcaacctgat tatgtcagcg gcggcgcaga cggtggcgcg
     7681 cgtcgaactc ggcgcaaact tgcagggcgg cgacgcgcgc acgatcaacg aatggatcgg
     7741 acttgcgccg gattcaaaac tcgtgcgcct gcaccgcctc gacaacgtgg cggccacgcc
     7801 cgcttccggt tcgcacccgc aggcgggcat gctgatcgtc ccctgctcga tgggcacgct
     7861 cggcgcaatc gcttccggcg caggcacgaa tctgattcac cgcgcggcag acgtgacttt
     7921 gaaagaaggg cgcaagctcg tcatcgtccc gcgcgagtcg ccttacaacg cgattcatct
     7981 ggagaacatg ctgcgtctgg cgcgtgcggg tgcgcgcatc ctgcccgcca gtcccggctt
     8041 ctatcatcgc ccgcagagca tcgaagagtt ggtggatcat ctggtcttcc gcatactcga
     8101 tcagttcggc gtgccgcaca cgcagcgcac gcaatggcag ggcgacccga cggcgaccac
     8161 gaccgcgccc gtcgccatag aaggcgactg aacttggcac tatgcgaccg ggcgacccta
     8221 tgagttgcga attgcaaaat ttgataactg ccggtaacgg cgcgaactct ttctccgtgc
     8281 ctcttgctct gtgtttccgt gatgaagggc gtgcgccgga gtggctttaa cgcgcgctcc
     8341 cgctggcatc aatcttgcag tcagttttca ggcagacgta atttccgaca gcagcaaccg
     8401 gatcgacttt cgatgagttc tcaggcaacc aacacgacga cggcggcggg caacctgcgc
     8461 acgacgctcg aaatgatcaa gatcgagcac acgctctttg cgctgccctt cgcgctgctc
     8521 ggcgcggtgc tggcggcgcg cggcgtgccc tccgtgtggc agttgctctg gatcaccgtg
     8581 gcgatggtgg gcgcgcgctc cgccgcgatg gcctttaacc gcatcgccga tcacaggtac
     8641 gatgcagcca acccgcgcac cagcgggcgc gcgattccgt cgggtgcgct ctcgctgaaa
     8701 ttcgcgtggg cgttcacgct ggcgtcggcg tcgctcttct tcgtcgcggc ggcgatgctc
     8761 aaccgcctga cgctcctgct cgcgcccgtc gcgctggcga gcgtgcttct ctattcctac
     8821 acgaagcgtt tcacatcgct ctcgcatctc gtgctcggct ggtgtctggc aatcgcgccg
     8881 acgggcgcat ggatcgccgt gcgcggtgcg tttgacagcc ccgtgccgct cctgctctcg
     8941 ctcgtcgtga tgctctggac agccgggttc gacgtgctct acgcctgcca ggattacgac
     9001 ttcgaccgcg cggcgggttt gcgttcgatc cccgcacgcg tcggcatcgg gcgcgcgctc
     9061 tggatttcca gactactcca tgcggcagcc ttcgccgcgc tcgtcgcgct ctacctgacg
     9121 gccaagcccg gcgcgttcgc gctcgccggg ctggccgcga ccggcgcgct gctcgtctat
     9181 caacattcac tcgtccgcgc ggacgatctc agtcgcctga acgccgcgtt cttcaccacg
     9241 aacgccttcg tcagcgtcat ccttttgctc accttcggcg gcgcggtctt cttcgcccgg
     9301 ctgtgagtcc ggaggagagg ttcagcagtt tgacccggaa agaaattctt atgagcacaa
     9361 acacttcaat ccctaccgaa cgcgaaccca tcgtgctgca cttcagccca cgcctgaagc
     9421 agataagcga ggaagagttc tacgaattct gtcaactcaa ccgtgatttg cgcctcgaac
     9481 tcactagcga aggagactta atcattatgc ctccgacggg cggaaagagc ggcttgcgga
     9541 atgcgacgct gacggcagaa ctcaaaaatt ggggaaagca ggacggcaca ggcttagtct
     9601 tcgattcatc gactgtgttt gcgctgccta acggcgcaaa gcggtcgccg gatgcgtcgt
     9661 gggttagcaa agaccgttgg agcgcgttgt cggaagatga gcaggaacaa ttcccgccgc
     9721 tttgcccgga cttcgttgtg gaactgcggt cgcgcaccga cgcgctcaaa aatttgcgac
     9781 agaagatgga agagtacgtc gctaacggcg cgcaactcgg ctggctgatc gatccgtcgg
     9841 agcgaaaggt ctacgtctat cgtccgtccg cgcccgcaga ggaattggat aacccgcaga
     9901 caatttccgg cgagcctcta ttgcgcgggt tgtcgctcaa cctacaggag atttgggact
     9961 aacatcttcg tcgcgcgagg cgcgacaaat tccaatgaca gttatgaact tttcacacga
    10021 cccgaaactt aaagagatcg ccgcaaaagt agaagccgga gaacggctct ccttcaccga
    10081 cggcatggac ttatatgcga ccgacgatct ggccgcgctc gcgaagctgg ctgacacggt
    10141 gcgtcgtcgc ctgcacgggc ggcgcacgta ttacaacgtc aatcgtcatt tcaactacac
    10201 gaatgtctgc tacgccgact gcaagttctg cggcttctat cgcacgccgc gacagcccga
    10261 cgcctacacg cacaacgtcg aggaggccgt cgcggtggcg ggcaaggcgg tcgccgaggg
    10321 cgcaaccgag ttgcacatcg tcggcggctt gaacacgaaa ctccctttca agtactacac
    10381 agacctgctc gcaaacctga agcgcgccta cccgcgcctg cacctgaaag ccttcacgat
    10441 ggtcgaactc gaccacatgg caggcttcta caagatgagc gacgaggagg tgatcgcgcg
    10501 tctgaaagag gccgggatgg attcgtgtcc cggcggcggt gcggagattt ttcgcgagcc
    10561 gacgcgctcc agaatttgcg cgcacaagac ggacgccgga cgctggctcg cgctcgcggg
    10621 caaagttcac gacgcgggat tgaagaccaa cgcaacgatg ctctacgggc acatcgagtc
    10681 cgtcgaagac cgcgtagacc acttcataca actgcgcgag cagcaggacg catcgggcgg
    10741 cttccagtgc ttcatcccgc tcgccttcta tcccgaaggc acgaagctct ccgcgtgtcc
    10801 ggggccgtcg ggcgtggatt cgttgaagac catcgccgtc tcgcgtctca tgctcgacaa
    10861 cttcccgcac atcaaagcct actgggtgat gctcggcaag cggctcgcgc aggtcgcgct
    10921 gcactacggc gcaaacgatc tggacggcac gatcacagaa ggcggcgaac tcaccgaaag
    10981 ctactcggtc gagtcgaaca atgaagtgcg tatgaacaaa aacgagatcg ccgccctcat
    11041 ccgcgaggcc ggattcgaag ccgtcgaacg cgacacgctc tacaatccaa tcgtggagat
    11101 gacggcggct tgagattcgt cccgttcttc tttgcgctcc cggcatgagc ttacgagctc
    11161 gccaggacgc ggaggaaacc ttgagcgtta taaagatgga agtggcgatg actgagattg
    11221 agccgacgaa gagattcacc gaacgcgtgg cggactacgt ggcttatcgc ccgaagtatc
    11281 cggcggcggt cttggagttc atgcgtgcgg agttgaatct gcgcccgtcg tcaatcatcg
    11341 cggatgtcgg gtcgggaccg ggcattctga cggagatgtt tttgcgcaac ggcaacacgg
    11401 tcttcgcggt cgaaccgaac gaggcgatgc gcgccgccgc cgaaaattta cttggcgcgt
    11461 ttcccaattt taagagcgtt gacggcatgg ccgaggcgac gacgcttgac gcgcgaagcg
    11521 tagacttcgt gacggcggcg caggcttttc actggttcga cgccgggcgg gcgttggcgg
    11581 aattcgcgcg catcctgaaa gacggagggt gggttgcgct gctctggaac atgcgccgca
    11641 cagactcgac gcccttcctg cgcgaactcg aacgcatcct gcgcgcttac ggcacggact
    11701 acgagcgcgt cgccgccgac aaccccggac aggagttgat ggaacgaatc ttcccgcgcg
    11761 gcttcggcac gaagaggttc cggcacgagc aagtcttcga ctatgcgggc ttgcgtggcc
    11821 gctggctctc cgcctcctac gtgccgctcg cgggacaccc gaatcacgaa ccgatgttcg
    11881 acgcgctccg gcgcgccttc gacgagcaca acacggacgg caccgtcgtc atcgaatacg
    11941 acacggtgct ctactacagt caactctcta aagtctgaca gagcatggcc tgaacttgct
    12001 cgcaaacaac cgccgacgaa caactgacaa ttcacaactg acaaccgaca accatttatg
    12061 accaccgaca cccgaacgca aaccatcacc gtcgcgcact cgcccgattc cgacgacgcc
    12121 ttcatgttct acggactcgc caccaacaaa ctcgacacag ggagcctgcg cttcacgcac
    12181 gtcctcgaag acatccagag tttgaacgag aaggcgatgc aaggcgtcta cgatgtgacg
    12241 gccatcagct ttcacgccta cgcttacatc tctgacaagt atgcgctgtt gccgcacggc
    12301 gcgagcatcg gcgacaacta cggccccatc gtcgtctcgc gtgaaaagtt caagccggaa
    12361 gaactctcgt cgttgaaagt cgccgtgccc ggcaagctga cgagcgcgtt cctcgccctg
    12421 agcatcttct gcccggagtt tgaatatgag atcgtgcctt tcgataagat catcgaggcg
    12481 gtgcaggagg ggcgcgcgga tgcgggtctg ctcatccacg agggacagct tttctatcaa
    12541 aacatggggc tgcacaaagt cttagacctc ggcgagtggt ggcacgagcg gacggggctg
    12601 ccgctgccga tgggcggcaa cgtcatccgg cgcgacctcg gcgcggaagt gatctcgcaa
    12661 gtctcgaaat gcctgcacga gagcatccga tactcgctcg acaatcgcgc ggatgcgctc
    12721 gaatacgcga tgcagtttgc gcgcgacatg gaaccggagt tggccgaccg tttcgtcggc
    12781 atgtgggtca acgaactgac gctcgactac acggagcgcg ggcgcaccgc cgtccaacgt
    12841 ctgctcgacg aaggcgcaga gcgcggcatc atcccgcgcc gcgtcgacgt cgaattcgtg
    12901 cagtagaata gacggtgagc agtgagcggt aagcggcaag gcattcaggg tcgtgcccct
    12961 gcggcttgcg gctcactttc ttctggcaac attcgccgtg cgggttgcat ctcagtcttt
    13021 ccaagaaggg tcgctcggct ttcctgtggc gcgggcggcg gcggcagtaa gactcaaatt
    13081 cccagagaaa atttatgaaa aactctcttt cgtggcaatc gagattgagg ttgtttgtgc
    13141 tgctgctggc gtgcgccgct tttgcgcccg ccgcaggggc gcagcaagtc tcttcggcga
    13201 gcgcgccttc cgcttcgacg gcgggcgtcg atgtcgaacg catcatacgc gagttcaccg
    13261 ccaaagaggc gacgttccgg caggcgctca accagtacag cttcaagcgc gaggccgtcg
    13321 tgcagactat cgggatgggc gggcagattt ccggcgagta tcgccgcatc tcgcgcttcg
    13381 tcttcgacga cagcggcaag cgttacgaga agattctgct gtttccgctc ccgaccctca
    13441 ccgaagtgga actgtcggga gaggatttgg aagacctgag tggcgtgcag actttcgcgc
    13501 tcgaaccctc caagatcaac ctctatcaac tcacttacgt cggcaaggag aagattgacg
    13561 aactggactg ttacgtcttc gacgttgccc cgaaggtgat gcccgacccc aagaagacga
    13621 aagaccgttt cttcaagggg cgcatctgga tcgacgatca agacgtgcaa atcgtgaaag
    13681 tgcgcggcaa gggcgtgccc gaaaaaaaga acgcgcgcta cccgacctac gaaacctacc
    13741 gcgaacagat cgacggcaag ttctggtttc cgacttacac ttacgcagac gaagaactga
    13801 tcttcgatag cggtcaggtc gtccgcctgc gcctgcgcat caccttcaac gagttcgtcc
    13861 gccccagcgc gaaagtcaga gttatcgacg aaggacagga catcatcccg acgccttcgc
    13921 ccacgccgca gcagcagaag aaaccttgaa cgcagaggtc agaggctaga gtttagagta
    13981 tcggtaaaaa caggcaggcc ggggaacgca gcttagtcgc tcgctcctcg gcctgcatct
    14041 ttttccaacc tccaaactct agcctctaag ttctagtctc tgaactctgt tcttagttct
    14101 tccgccacca gttcgcgcac cgtcccggcg aggcgtctga catcttcgtc cgtcgagagt
    14161 ccggtggttt cgacgggcgg gagaataacc atttcaatcg ttccggcgcg ggcgacgccg
    14221 gtgcctttgc ccatgagcgc gtccgtgttt ttgatggcga cgggcacgat gcttacgccc
    14281 gtctctatcg ccatgtagaa tgcgcctttt ttgaacggca gcaattgacc gggtagtgcg
    14341 cgcgtccctt ccgcgaagac gccgaaggag acgccggagc gtatgcgttc ggtggcggcg
    14401 cgcaccgttg ccagcgcgcg ttcgcggttg gaacggtcaa tcgccatgat gttcacgaag
    14461 cccattccga agccgagaat gggaactttc aacaactcct ttttagcgaa aagtccgatg
    14521 cgccgtttga ggtggcagaa gagagtcgct gtgtctagat aggagcggtg gttggcggtg
    14581 aagatgtaag tgcggcgcgg gtcgagttgt tccagaccgc gcacgcgcac gcgcatcccg
    14641 ctcaggcgca gccattgccg cccgccccac agggcaatcg ggtagaccca ctcccgccgt
    14701 cccgcgagcc acgccaccaa cagcaccgga ggcccgaaga tgagcagcag catcgccgcg
    14761 acgaacagaa tccaccagtg atatagacga ccggcgaagg acgactcccc cgcgcctccc
    14821 gcgacgaggc ggcctgcggc ggcggcttct cctccggcca tcgcgtcctg cttcgaggtg
    14881 cgcggcgcgc tcgcgtctgt gctagactcg cccccggcac acacgctctc cgtatcaaac
    14941 aatttttccg aaagagaatc agtcatgtct cgctccgttc tccggctcgc gctctcgact
    15001 tatctcgtcg cgctctctct ggtcgtcgtc ctctgcggcg cgctctttgc gcccgccccg
    15061 gttcgcgcgc agcagacgcg ccgccccgct tcccccgcgt cgcgctctgc cgcgacgaca
    15121 actaacgcgc cgccctcgcc cgcgtccgtg ctcggtttca cgcccggcga cgaccgcacg
    15181 gtcgcagact ggcgacagat cacagactat ttcacacgcc tcgatgcggc gagcgaacgc
    15241 gtcgaggttc gcacgctcgg gcagacaact ctgggtcgcc cgctcgtcgt cgccttcatc
    15301 agcgcgcctg agaatatacg caatctcacg aagttccaag accttcaacg ccgcctgtcc
    15361 gacccgcgcc tcgccagcac ggaagcagcg cgcgacgagt tgacgcgtga aggcaagacc
    15421 gtcgtggtca tctcctgctc gatccactcg accgagatcg tcgcctcgca gatgtccatg
    15481 caactcgcct acgagttggc ttccgccgca gacgccgcga cgcgcgagat tttgcagaac
    15541 acgatcctcc tgctcatccc tgcggccaac cccgacggcg tggacatcgt cgccaactgg
    15601 tatcgcaaaa ctctgggcac gccctacgaa ggcaccgaac cgcccgagct atatcatcat
    15661 tacgccgggc acgacaacaa ccgcgactgg ttcatgctca acttaaaaga gacgcaactc
    15721 ctgacgcgtc tcttctggcg tgagtggttt ccgcagatag tctatgacgt gcaccagcag
    15781 ggcgcgaacg gctcgcgctt cttcgtcccg ccctttttcg atccgccgaa cccgcacatc
    15841 ccgccgctcc tgctccgcga agtcggcctc atcggccaca agatcgccgc agacttgcag
    15901 gccgcagggc agcagggcgt tatcaccaac gcactctacg atacatggtg gcacggcggc
    15961 tttcgcaccg cgccctatta tcacaactcc gtcggcatcc ttaccgaagc cgcgagcgcg
    16021 cgcctgatgt cgcccgtcga agtgacgcgt gaacaactca cgcgaagcac cgcgcgcggc
    16081 atgccgagcg cgctccaatc ggcgaccaac tttccagacc cgtggccggg cgggacgtgg
    16141 cgaccgcgcg acatcatgcg gatggagttg acggcctcgc gcgccgtgct ctcgatggcc
    16201 gcgaagtatc gcgagcgtta cctgcacaac ttttacgaac tcggtcggcg cggagtcgaa
    16261 gggagaaaca cagtcggcga cgccgagatc ggatatgtga ttccggcagg gcagggcaag
    16321 gacgaggccg tcgcgaagtt gatcggcgcg ctcgtcgaac agggcgtaga ggttcaacgt
    16381 ctcgacaaag aattgcacct gatgatgctc ggcaatcgcg gcgcggtcta taacgatctg
    16441 gtacagaacc cgcccgtcat gcctgcgggc agttatctca tctttctcgc gcagccttac
    16501 cgcgccaaca tcaaggctct cttccaaaag cagatttatc cggaccggcg caccgccggg
    16561 ggcgaggccg aacgccctta cgacgtggcc gggtggacgc tcccgatgca gatgggtgtg
    16621 caggctctcc ccattcacag catccgcgaa gccgcatccg aaagacgcct gacgctcatc
    16681 cgcgatgcga acgaagtgcg gcgcgcgctc aacctgcccg cgcagcagga tacgcgctcg
    16741 cccctcacga atcccgtcgc gcgcccctcg cgccttgcgc tctacaagag ttggacgggt
    16801 tcgatggatg agggctggac gcgctacgtc ttcgacacgt tcaacgtccc gttccagaca
    16861 ttactcgaca aggaagtgcg cgccggaaac ctgcgcgccc gttacgacgt gatcgtgctg
    16921 ccctcgatgc gtctcaggga gatcgtggaa gggcgtagcc gggagactgc gccgccggag
    16981 tttgcgggcg gtatcacgga ggcgggcgtg gagaacctgc gccgcttcgt cgaagacggc
    17041 ggcacgctcg tctgcttcga cgctgctgcg gaactcgccg tcaagcgttt caaattgccg
    17101 ctcagaaacg tcctcgaagg cgtgcgttcg tcggagttct acggccccgg ctcgatcctc
    17161 cggctcgaag ttgataccgc gcacccgctc gcgcgcggtt tcggcaaaca ggtggacgct
    17221 tatttcatca acagttccgc ctacgagttg acggcggacg cggagaagcg cgtgcgcgtc
    17281 gtcgcgcgtt atgccgaacg cgacgtactg cgttccggct ggctgctcgg cgaaaagctg
    17341 atcgcgggca agatcgcgct cgcggaagtg aatctgggca aagggcgcgt cgtccttttc
    17401 gccttccgcc cgcaacaccg cgcacagacg tggggcacct tccccttcat ctggaacgca
    17461 atcgagctaa gggatgaggg atgaatcaga acaaagcagg ctgttgaatg ctttctctta
    17521 ttcatccctc atccctcatc cctcatccct tttatcgggc atctattacc cggcgcgcgc
    17581 atcaaagttt gcgtccgcgc ctcgtggtcg cttaatatcg ctttagttaa aaggagttta
    17641 gaaaggccat gagaaagttc gccgccttgc ttgcactcct ctccctgctc gcaacttccg
    17701 cagcttcaat caacgcgcag acgcgcccgc gtcgcgtagg gcagaccaca ggccgcgcaa
    17761 atccgcctga cagcagcagc actactgcgc cccgctccga cgccgaaaat gatcgcggcg
    17821 cgcggcgtcc gccgacgctc ggcggcgcag tcggctccaa cgacaccgcc agcgcacgca
    17881 cctcgaacac gacagacccg accgaagttg acgagaacga agttgtccgc gtgaacactt
    17941 cgctcgtgac gattccggtg agtgtgatgg acagggcggg caagtacgtg ccgagcatcc
    18001 ggcaacagga gtttcgtatc tgggaggaag gcgtcgaaca agagatcgcc tatttcgcca
    18061 cggtcgaaaa acccttcacc gtcgccctcg tcattgacac ctcaggctcg acgaagtttc
    18121 gtctgcgtga gattcaggac gccgccatcg ccttcgtcaa tcaactgcgc cccgaagacc
    18181 gcgtgctcgt cgtctctttc gacgacgagg tgcgggttct ctgtgaagcg acgagcgacc
    18241 gcgctcgact gcacgacgcc attctccgcg tctaccccgg cgagggcacg aagctctacg
    18301 acgccgtcga tttcatcatc aatcaacgct tcaaccgcat cgaggggcgc aaggccatgc
    18361 tcctcttcac cgacggcgtt gacacttcga gccgtcgcgc cagctatgaa tcgaacgccc
    18421 gcgacgcgga agaactcgac gcgctcatct atcccgtcca gtacgatact tttgccggtg
    18481 gcaacagccc gagcggcaac ggcggcggcg tctggggcgg cgggaaccgg cgcagacgcg
    18541 gcggcatggc agacattctc tcggatattc tcatcggcgg aaacgttcgc atcggcggcg
    18601 gaagtggagg cggcagaggc ggcagtgggg gtggaggcgg cggtaacagt tgcacgggct
    18661 gcacgcgcga agagtacgca cgcggcaacg cttacctgaa cgacctcgcg cgtctgtccg
    18721 gcgcgcggct ctatcgcgcc gacaccacgt acgacatcgc gggcgcgttc tcgctcgtgg
    18781 cagaagaact gcgtcgccag tacagcctcg gctactaccc gaagcagcag gggcagcccg
    18841 gccagcgtcg ccgcatcaaa gttcgcgtca tgcgccccga cctcgccgtc aacgcacgcg
    18901 acagctacat ctacaatcca tccggcgcaa acaacaacac caccaccgcg caggactcca
    18961 accagcaacg ctcccaaccc gtactgcgca ggtcgccgct cgcggaaaga taaatagtga
    19021 tgagtaatga gtgatgggtg atgagttaaa gcagt
//
LOCUS       DASWNS010000397        14215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_144147, whole genome shotgun sequence.
ACCESSION   DASWNS010000397 DASWNS010000000
VERSION     DASWNS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14215
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144147"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VGB76_13885"
     CDS             <1..668
                     /locus_tag="VGB76_13885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEY0100035.1"
                     /translation="EDSITNFGDELAGEMAYAFSQSEDECGFLGDATSTYHGIMGVIP
                     RLVELDDAVANIAGLVEATGATWDSITRADMLAVAERLPEYADTPETKWYCSRKFYVN
                     VMMRIMLEAGGVTAAHLESMRVKMFLHYPVEIAQVLPKKSAASQVPCLFGDLRQAARF
                     GDRRQISVDVTDANRTEFEEDVLTIRGTERFDINVHDVGNASADVDEREAGPIVGLIT
                     KSS"
     gene            693..968
                     /locus_tag="VGB76_13890"
     CDS             693..968
                     /locus_tag="VGB76_13890"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0100036.1"
                     /translation="MSRGLEQIFSPETLAALDDYVSAKVREGLKESLPEPEGGAEYIS
                     VKTASARFDISESTIRDWIRIGKLRKYKIMGCVRLKPSEIVRELENQ"
     gene            1020..2108
                     /locus_tag="VGB76_13895"
     CDS             1020..2108
                     /locus_tag="VGB76_13895"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0100037.1"
                     /translation="MGVYKRKGKWEVRLQMNGVRYYRQVPEAHNKGQALVAEATLRKE
                     IYEGRYGKEGGEIGTTDFKEFCERVYLLDIRNRLVQWKNEQYKVRTLCRFFAGKRLRD
                     ITPMLVQGYKRQRVSGETQYGRKRQASTVRGEIMTLSAIFNMAIDNDLIGANPCRKVK
                     WARGEATSRRDRVLTLDEERSLMPQIESFPEVKAAVVLALNTGLRRMGILTLKTSDVN
                     FAGRTVIYTAKGGRRRTLPLNAEALAVLQEMAKSPAPGGFLFHNRTGNNLSAAGGAFK
                     LAVERAGLQDMNFHDLRHTFATRVRLHADAYTVRDLLGHSKVDTSNIYVNTAPLEEQR
                     GAVDALVKHPRVLEAAEKFHRAFTANDR"
     gene            2233..2793
                     /locus_tag="VGB76_13900"
     CDS             2233..2793
                     /locus_tag="VGB76_13900"
                     /inference="COORDINATES: protein motif:HMM:NF021577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HEY0100038.1"
                     /translation="MKEFSMFEFALKAAKDSQLEDLGQLAIVSRKNFDERPTFRFRAQ
                     VPCLEYVSLLIENALLLRTNREGRVYAGFEKLSRMEYVADRFLRISDVSERVYVFGVP
                     DWKPPRHPNLGIVRLKPDTDLAREWFVIAHSQKFNAALVAVDEDGFDTPILDERNFRS
                     FKTTDPRIVAQLAAAAEDLIDQSVKS"
     gene            2897..3646
                     /locus_tag="VGB76_13905"
     CDS             2897..3646
                     /locus_tag="VGB76_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100039.1"
                     /translation="MRRLIIVAALSLAALSLAASTHEAATRAPFVSLAQAFYPTGSQT
                     AQAPKGWSLTGSHREHYYLTTDNKVRRGGKLSATLISKNSASDDGFGSMKQEIKADNY
                     RGRRVRYSGYLKAELAAQRAALWMRVEGADGKILAFDNMEERPATGTTAWKKYEIVLD
                     VPEAAQHIALGALFEGKGQIWVDDLKLEVVGQEVASTDMNASPELKQEMEEADEEYRR
                     TRREEFERAVRQAKERLSSMPERGVNFDFEG"
     gene            complement(3734..4855)
                     /locus_tag="VGB76_13910"
     CDS             complement(3734..4855)
                     /locus_tag="VGB76_13910"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0100040.1"
                     /translation="MMRRLLRSLSFVIALQCALAALVVAQPPVPRSLDGHDEIRVLTE
                     EVRIPFTALDDYRRFDPSVELHDLLLREDGVVQQLRSLYRIPASVLLLLDTGGQRNIA
                     ASVRLTREVALRLVSKLQAEDSLALVQVSERVELLQDWTKERARVVRALNNRLFPGRR
                     TAFSQGVAAAVEQLGRTPAGNRHLIMITEGIESVYGESEYEAAIKSLIEANVTVHVLS
                     YTSLGRSAPAPRATRQRVDAGVPEEILMTLPRYRKPEAIDMVDQLRRKGGTTIAIERL
                     FKRDSEYRAARDRGELELTGIAGETGGGMWLPISTGEMLGHADEVARDVDARYVVTYR
                     PHRPLAPGGAKEYRRVDLISRRGGLSVRARRGYVSSGAQ"
     gene            complement(4951..5982)
                     /locus_tag="VGB76_13915"
     CDS             complement(4951..5982)
                     /locus_tag="VGB76_13915"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0100041.1"
                     /translation="MLRPLNEQVVVVTGASSGIGRATAIEFGRRGASVVLAARNAEAL
                     STAAREVEAAGGGARTLVVQTDVADSAQVLRLAEETVNRFGRIDTWVNNAAVSEYGTI
                     EKISIEEIERVVQVNLLGTIYGMRAALPHLKRAGGGAIINVGSVLSDRAVPLQSTYCA
                     TKHAVKGFTEAVRMELEHERSGVHLTLIKPAVINTPYYNHARTRMGVEPRAMFPVYEP
                     SLVAEAIIYAAAHPVRDIVVGDGGKFLCVLQRLSPSLVDRMMLLGGAAFKLQQRDEPA
                     RPGDNLFTPMNEAGATTGEYTHRSTDFSPFTRHLEPYPNRKRLLVAAAAFGAFALLRR
                     GGARRPTRA"
     gene            complement(6000..6494)
                     /locus_tag="VGB76_13920"
     CDS             complement(6000..6494)
                     /locus_tag="VGB76_13920"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY0100042.1"
                     /translation="MESKFKVLGHGAHPILIVFPLGLLATAAIFDIIYLLTNNQQIAN
                     AAYWMIAAGVVGGIVAAVPGFVDWWAIPKGTRAKRVGLIHGVGNDVVLILFGLSWWLR
                     RDTINHVPETVPFILSMLGVGLSLVTGWLGGELVERLGVAVHDGAHLDAPNTLSGRPA
                     RERE"
     gene            6903..7916
                     /gene="pta"
                     /locus_tag="VGB76_13925"
     CDS             6903..7916
                     /gene="pta"
                     /locus_tag="VGB76_13925"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEY0100043.1"
                     /translation="MSILRRIRERAARDPQRIVLPEGEDPRTIVAAAICARERLAHIT
                     VLGNEEKVRAAQREAGVDLGGMVQVIDHTRAADFNKVAAFYHELRKAKGLMPDEARIA
                     IQDPLYYGNLMVRAGAADGSVAGATNTTAHTVRAALHCIGVRAGFKLVSSFFVMAHPD
                     PQFGAEGAMIYADCGVVIEPTAAELAEIAIASADSCRALLEVEPRVAMLSFSTKGSAK
                     HKLIDKVVEATRTVRARMPELEIDGELQADAALVPAVANSKAQGSSVAGRANVLIFPD
                     LQAGNIAYKLTERLAGASAIGPILQGLDKPCNDLSRGCKAEDIADAVAITAVQAQSRK
                     GQT"
     gene            8028..8162
                     /locus_tag="VGB76_13930"
     CDS             8028..8162
                     /locus_tag="VGB76_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100044.1"
                     /translation="MLGEAGMLSAQLAGADRRPFGMNRPGLDNRQLALNIMRWLSRVL
                     "
     gene            8250..9488
                     /locus_tag="VGB76_13935"
     CDS             8250..9488
                     /locus_tag="VGB76_13935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0100045.1"
                     /translation="MYTGRRLFWPFALVLLFCSAGTKLHAQSGRVIPSPTPAEDQIKV
                     YTEEVRLPVFARDEYGRFDPTLELDDLVVLEDNVKQEVRSIQRIPASVVLVLATGGEL
                     NPAQRTRTTREAALSLLSHLRAGDSVAVVQFDRKINLLQPWTVDRDAAAHTLRTKLWG
                     ASGARPAEALQRAAELFADQPVGNRHLVFVTDGVETPGGGATPEQITRVLEGNAGIGG
                     RTAYAEAVKKLMAAQVSVHIISYTEFGRNETREKKKNAPLSNAVPGSVKASGIATAGI
                     DPTMPPTMNRGGAVGPSTGAVITFDPAMKRLRKAYERAMKRGEESMKTLAADTGGRLW
                     IPLSMEDLALQGSEVAREIGTQYVVTYTPRRPLAESDATETRRVVVLPRRNGLQLRTR
                     RVYVAAAAMQKPPATKADAK"
     gene            complement(9617..11362)
                     /locus_tag="VGB76_13940"
     CDS             complement(9617..11362)
                     /locus_tag="VGB76_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0100046.1"
                     /translation="MKRVTHVARPKIAWLFVLLVALASQSPAALAQDKAARIDEYMTR
                     ANKLRQFNGAVLVAENGKVIYKKGLGLANMEWNVPNEVDTKFRLGSITKQFTATLIMQ
                     LVEQGKLKLDGKLSEYLPDYRKDVGDKVTIHHLLTHTSGIPSYTSLPNFMAEVSRNPY
                     TPVEFVKKYASGDLEFEPGAKFRYNNSGYFLLGAIIEKIHGKSYEQVLKENVFDPLGM
                     KNSGYDHYDAVLAKRASGYEKTPTGYRNAPYLDMSLPYAAGSLYSTVEDLFLWDQALY
                     TDKLISAKSKELMFKPFLDNYAYGWGITKVTLAQSKEVLNSVQHSGGINGFNTLLVRY
                     PERKQLIVLLDNTSQGGSLNRISRDLTNILHNEPFELPKQSIAETLMKTIMEQDVAAA
                     VKQYRELKTTQAANYDFNENALNALGYQLLGQKKSKEAIEIFKLNVESYPQAANPYDS
                     LGEAYLQAGERALALQNYKKAVELNPQNVAAVQIIKNMEAPPSTAPVALEAYAGQYEV
                     PGMGVLDITKDGDKLVGAPVGQPKAELIAQGNDRFLAAGPNVNLTFVKDDKGQVTHVN
                     VRLRDGREMLAKKVK"
     gene            complement(11507..12973)
                     /locus_tag="VGB76_13945"
     CDS             complement(11507..12973)
                     /locus_tag="VGB76_13945"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0100047.1"
                     /translation="MLIERALAACLFLLAFCAPHSIAGTQIAWGVGLLLWVLRFTARP
                     RPQLQRTPVDYALLGFFILTFISALASYDPDVSIGKLRAASLFTIVYLVAQNVASRRV
                     LRLLTLTLVASCMINVVYTFGQRAAGRGVKVAKLTADSPLRAAGINEGDTLLRLDRAP
                     CNSPEAITRALAANVTGMPQARVLVYRREWVFETELARGSLLAGATPEAQLGLTSWTR
                     GRDERASGFYGHYTTYAEVLQLIGSLAFGLLVAHRRKLSWQGALLAASVAGIGAALLL
                     TVTRASWLSFLLSIVLISLVGVGSRRAVLAVALGALLVIPLGLYVLQQKRGVGFFDRR
                     DGSISWRTTVYREGFDLFVREPRHVLVGVGMDSLKRHWREWGLFDGGRLPVGHMHSTP
                     LQLALERGIFALLLWLAVVFLYARMLWRLWRAPGLEDWRERGLVLGALGGLTGFFASG
                     LVHYNLGDSEVVMVFYLIMGLTLALERFAGQQAHAETR"
     gene            complement(12963..>14215)
                     /locus_tag="VGB76_13950"
     CDS             complement(12963..>14215)
                     /locus_tag="VGB76_13950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY0100048.1"
                     /translation="SYVDDALRVFKATAVASLLIIAVAFLYRGGTAFRAFSYSRAVFV
                     LDFAFALAAFGALRMGVRAAQAAVRRRGVNLIPTLVVGRGAEASLCIREMRERPELGY
                     RVIGVVENGAIQASVPETFEGVPVIASVRDLAETIRETAANEVIVADAGVSGDLLFDV
                     MMRVGRLRGVEFRIAPSLFNCLPRKTEIDQIGALPMIQLFREPLSNSARILKRTMDIL
                     VASFALALLAPLWLVIAVLIKLDSKGGVFYKQERVGMDGRIFLFLKFRTMQSGSDDAA
                     HRDYQRNYIAGRAETNLGDDERPIYKLRTDPRVTRVGRVLRRLSLDELPQLLNVLRGD
                     MSVVGPRPPITYEVEAYELWHRKRLDMKPGLTGLWQVSGRNRLSFEEMVRLDLFYIEN
                     WSLLLDLKIILRTLPVMLRGDNAY"
BASE COUNT         2799 a         4342 c         4571 g         2503 t
ORIGIN      
        1 cggaagactc gatcacgaac ttcggagatg agttggcggg agagatggcg tatgccttct
       61 cacagagtga agacgagtgc ggcttcctgg gagatgccac gagcacctat cacggcatca
      121 tgggagttat cccgcgcctg gtcgaactcg acgatgcagt ggccaacatc gcggggctgg
      181 tggaagcgac cggagccacg tgggacagca tcaccagagc ggacatgttg gcagtggccg
      241 agcgtctgcc ggagtacgcc gacactccgg agacgaagtg gtattgctcc aggaagttct
      301 acgtcaacgt gatgatgaga atcatgctgg aggccggagg cgtgacagca gcgcatctgg
      361 agagcatgcg ggtgaagatg ttcctgcact acccggtcga gatcgcgcag gtgttaccga
      421 agaagagcgc ggcctctcag gttccctgcc tgttcggaga cttgcgccag gcggcaaggt
      481 ttggtgaccg gcggcaaatc tccgtggacg tgacggacgc gaaccggacg gagttcgaag
      541 aagacgtgtt gaccatcaga gggacggagc gcttcgacat caacgtgcac gatgtgggca
      601 acgccagcgc cgatgtggac gagcgcgagg ccgggccgat tgttggcttg attacaaaga
      661 gcagctaagg gtgcgtgggt gaggtgtaag cgatgagcag aggactcgaa cagattttca
      721 gccccgagac actggcggca ctcgacgact acgtgagcgc taaggtgcgc gaagggctga
      781 aagagtcgct gcccgagcct gagggaggag ctgagtatat ttcggttaag actgcctcgg
      841 cacgctttga catctccgaa agtactatcc gtgactggat ccgcataggc aagttgagga
      901 agtacaagat catggggtgc gtccgcctga agccctccga gattgtccgc gaactcgaaa
      961 atcaataaac tcacgcccgc tacgaagggc ggcgcttccg gccacacaga aggagccgca
     1021 tgggagttta caaaagaaag ggcaagtggg aggtccgctt gcagatgaac ggggtgaggt
     1081 attaccggca ggtgccggag gcccacaaca aagggcaggc tctggttgcc gaggctaccc
     1141 tgcgaaaaga aatttacgag gggcgatacg gcaaagaagg cggagagatt ggcacgaccg
     1201 acttcaaaga gttttgcgag cgcgtctatc ttctcgacat cagaaaccgg ctcgtccagt
     1261 ggaagaacga gcaatacaaa gtacgcacgc tctgccggtt cttcgcaggc aagaggctac
     1321 gcgacatcac gccgatgtta gtccaaggct acaagcggca gcgtgtatca ggcgagacgc
     1381 agtacgggcg gaagcgtcaa gcctcaacgg tgcgggggga gatcatgaca ctctccgcga
     1441 tcttcaacat ggccatcgac aacgacctca tcggcgcgaa tccatgccgg aaggtcaagt
     1501 gggcgcgagg cgaggcgacg agccggcgtg atcgcgtgct tacactcgat gaggaaagga
     1561 gtctcatgcc gcagatcgag agcttccccg aagtgaaggc tgcggtcgtt ctcgcgctca
     1621 acaccggact gaggcgtatg gggatcctca cgctcaagac ttcggacgtg aatttcgccg
     1681 ggcgcactgt catctacacg gcgaagggcg ggaggcggcg gacattaccg ctgaatgccg
     1741 aagcgcttgc cgtcctgcag gagatggcga agagtcccgc gccgggtggg tttctctttc
     1801 ataaccgcac aggcaacaac ctgtcagcgg cgggcggcgc cttcaagctg gcagtggagc
     1861 gggctggatt acaggatatg aattttcacg acctcaggca caccttcgca acgcgcgtgc
     1921 gcctgcacgc ggatgcatac actgtgcgtg acctcctcgg tcactccaag gtggacacgt
     1981 ctaacatcta cgttaatacg gcaccactgg aagagcagcg cggggcagtt gatgcactgg
     2041 tcaagcaccc acgtgtcctc gaggcggcgg aaaagtttca ccgggctttc accgcaaacg
     2101 acagatagca cgtttttcgg atgaatgtgc gccacctaca caatttaaat aaacattcgt
     2161 aacggcgtcg aagcggggcg ggagtagaag agtgttggcc gtgtgcggac gtgccggagg
     2221 ggtggttaca aattgaaaga gttttcgatg ttcgagtttg ccctgaaggc cgcgaaagat
     2281 tcgcaactcg aagacctcgg ccaactcgcg atagtctctc gaaaaaattt cgacgagcgc
     2341 ccgacattca gattccgcgc gcaggttccc tgcctcgaat acgtaagcct gctcatcgag
     2401 aacgcgctcc tgctgcgcac gaatcgagag gggcgcgtct acgcgggctt cgagaagttg
     2461 tcgcgcatgg aatacgtggc cgaccgcttt ctgcgcatct cggacgtgtc cgagcgcgtc
     2521 tacgtcttcg gcgtgcccga ctggaaaccg ccgcgccatc caaacctcgg catcgtgcgt
     2581 ttgaagcccg acaccgacct cgcacgcgag tggttcgtca tcgcgcattc gcagaagttc
     2641 aacgccgcac tcgtcgccgt cgatgaagac ggcttcgaca cgcccatcct cgacgagcgc
     2701 aacttccgct ccttcaaaac cacagacccg cgcatcgtcg cccaactcgc cgctgccgcc
     2761 gaagacttaa tcgatcagtc ggtcaagagt taaagacgca attcgagccg cgcacggatg
     2821 cggggacggc ggcgggaact agcttccacg cgcgtacgcg ttttaagatg tgcgcgtccg
     2881 acgcgaaagg aggcttatga gaaggctcat catcgtcgcc gcattgtcac tcgccgcatt
     2941 gtcgctcgct gcctcgacgc acgaggcggc cacccgcgct ccgttcgtct ctttggcgca
     3001 ggcgttttat ccaaccggga gccagaccgc gcaagcgccc aaagggtgga gcctgaccgg
     3061 gagccaccgc gaacactact atctgaccac cgacaacaag gtgcgacgcg gcggcaagct
     3121 gagcgcgacc ctcatctcga aaaactccgc ctccgacgac ggcttcggct cgatgaaaca
     3181 ggagatcaag gccgacaact accgtggacg gcgcgtacgc tattcgggct acctgaaggc
     3241 ggaactcgcc gcccagcgcg ccgcgctctg gatgcgtgtg gaaggcgcgg acgggaaaat
     3301 tctcgccttc gataacatgg aagaacgtcc cgccacgggc acgaccgcct ggaagaagta
     3361 tgagatcgtc ctcgacgtgc cggaggccgc gcagcacatc gccctcggcg cgctcttcga
     3421 ggggaagggt caaatctggg tggacgatct caagctcgaa gtggtggggc aggaagtggc
     3481 ttcgaccgac atgaacgcgt cgccggagtt gaagcaggag atggaagagg cggacgagga
     3541 gtatcgccgg acgcgcagag aagagtttga gcgggcggtg agacaggcga aggagcggct
     3601 ctcgtcgatg ccggagcgcg gcgtcaactt cgacttcgag ggttgagacg aaggttgact
     3661 tcaagggttg agaagaaggt tgaacttcga gggttgaggc gcgcttcgac ttcggggctt
     3721 gagtccgcca ctactactgc gcgccgctcg aaacatagcc gcgccgcgcg cgcacggaca
     3781 ggccgccgcg ccgcgagatg agatcaaccc tgcgatactc ttttgcgccg cccggcgcga
     3841 gcggcctgtg cgggcggtag gtgacgacgt aacgcgcgtc cacgtcccgc gccacctcat
     3901 ctgcgtggcc gagcatctcc ccggtcgaaa tcggcagcca catgcccccg cccgtctctc
     3961 ccgcaatgcc ggtcagttcc aactcgcccc ggtcgcgcgc cgcccgatac tccgagtcgc
     4021 gcttgaagag gcgttcgatg gcgatggtgg ttccgccctt gcggcggagt tggtcaacca
     4081 tgtctatcgc ctcaggcttc cggtagcgcg gcagggtcat gagaatctct tcgggcacgc
     4141 ctgcgtccac gcgttggcgc gtcgcgcgtg gggcgggcgc gctacgaccg agagaggtgt
     4201 aactgaggac gtgcacggtg acgttcgcct cgattaaaga tttgatcgcc gcctcatact
     4261 cgctctcgcc gtagaccgat tcgatcccct ccgtgatcat gatcaggtgg cggttcccgg
     4321 cgggcgtccg ccccaactgt tcgacggcgg cggccacgcc ttgcgaaaaa gcagtccgcc
     4381 tgccggggaa gagcctgtta tttaaagccc tgacgacgcg agcacgctcc ttcgtccagt
     4441 cctgcaacaa ctcgacgcgc tcgctgacct gcaccagcgc gagcgagtct tcggcttgaa
     4501 gtttggagac gaggcgcaac gcgacctcgc gcgtgaggcg cacgctcgcg gcgatattcc
     4561 gctgcccgcc cgtgtcgagc agcaacagga cgctggcggg gatgcggtag aggcttctga
     4621 gttgttggac gacgccgtct tcgcgcaaca gcagatcatg gagttcgacc gacgggtcga
     4681 agcggcggta atcgtcgagg gctgtgaaag gtatccgcac ctcttcggtc aagaccctga
     4741 tctcgtcatg gccgtcaagg ctccggggaa cgggcggctg cgccacgact aacgctgcca
     4801 gcgcgcattg cagtgcgatg acaaacgaga gtgaacgcag tagtcgtctc atcataatta
     4861 aacgcgtgtg ctatcccgtg ctatagacac gcggcggcga aatttggttg gcctcgttcg
     4921 ccgcacgcgt ccggggcgcg tcgcggggcg tcacgcacgc gtggggcgac gcgcgccgcc
     4981 tcgccgcagc agcgcgaacg cgccgaaggc ggcagcggcg accagcagac gcttgcggtt
     5041 cggataaggt tcgaggtgac gcgtgaaggg gctgaagtcg gtcgagcgat gcgtgtactc
     5101 gcccgtcgtc gcgcccgctt cgttcatcgg cgtgaagaga ttgtcgccgg ggcgcgcggg
     5161 ttcgtcgcgc tgctgtagtt tgaaggccgc gccgccgagc agcatcatac ggtctacgag
     5221 cgacgggctg aggcgttgca ggacgcacag gaattttccg ccgtcgccga cgacgatgtc
     5281 gcgcaccgga tgcgcggcgg cgtagatgat cgcttccgcc acgagcgacg gctcatagac
     5341 gggaaacatc gctcgcggct cgacgcccat gcgcgtgcgc gcgtgattgt agtaaggcgt
     5401 gttgatgacg gcgggcttga tgagcgtgag atgcacgccc gaacgttcgt gttcgagttc
     5461 cattctgacc gcttcggtga aacctttgac ggcatgtttc gtcgcgcagt aggttgactg
     5521 caaaggcacg gcgcgatccg agagcactga tccgacgttg atgatcgcgc cgccgcccgc
     5581 gcgcttcaag tgcggcaacg cggcgcgcat gccgtagatg gtccccagca aattcacctg
     5641 cacgacgcgc tcgatctctt cgatgctgat cttttcaatc gtcccgtact cgctcaccgc
     5701 cgcgttgttg acccacgtat ctatgcgccc gaaacggttc acagtctctt ccgcgagccg
     5761 taagacctgc gcggagtcgg ccacgtccgt ctgcacgacg agcgtgcgcg cgccgccccc
     5821 tgccgcttcc acctcgcgcg ccgccgtgct caacgcttcc gcgtttcgcg ccgccagcac
     5881 cacgctcgcg ccgcgccgtc cgaactcaat cgccgtcgcg cgcccgatgc cgctcgatgc
     5941 tcccgtcacc accacgacct gttcgttcaa tggcctcaac ataaattctc cgttcttccc
     6001 tactcgcgtt cgcgcgccgg gcggcctgag agcgtgttgg gcgcgtcgag gtgtgcgccg
     6061 tcgtggacgg ctacgccgag ccgttcgacg agttcgccgc cgagccagcc cgtcacgagc
     6121 gacagcccga cgccgagcat ggagaggatg aagggcacag tctccggcac gtgatttatg
     6181 gtgtcgcgcc tgagccacca actcaggccg aagagaatga gcaccacgtc gttgccgacg
     6241 ccgtgaatca gcccgacgcg tttggcgcgc gtgcccttcg ggatcgccca ccaatccacg
     6301 aagcccggca cggcagcgac gatgccgccc accactccgg cggcgatcat ccagtaggcg
     6361 gcgttggcga tctgctggtt gttggtcagg agataaatga tgtcgaagat ggcggcggtc
     6421 gccagcagtc cgagcggaaa gacgatgagg atcggatgcg cgccgtgtcc cagaactttg
     6481 aacttgcttt ccatagcgtt tgactccctc tcgtgtttgc agcgtgaaag acgaagcgac
     6541 gaagcgaggc tcttcttcac ttatcacaag ggggaagcga agaccggggg aacggtgtaa
     6601 ggggtgtgcg aagtgtagcc gaccgggcga gcgcacgaca agaggcgttt gtttccggga
     6661 agtaacattg tcgtcactta acggtagctg ccagcgtcga gttgtgaagc tgtcagctat
     6721 caatcgcggc gggagtttgc gtattgcgtc ccgcccggac gagcgaaccg cgccgcgtaa
     6781 cgcttgcgcg cgccacacgc gcggttatcg gtcgaaaaga agatgcacta tactggcgca
     6841 cgctcaatca tctgctgatc gccgattgct gatagctgaa agctaatcgc taactgctgc
     6901 ttatgtctat tctccgacgc atccgagagc gcgctgcacg cgacccgcag cgcatcgtcc
     6961 tgcccgaagg cgaagacccg cgcaccatcg tcgccgcagc catctgcgcg cgcgaacgcc
     7021 tcgcgcatat cacggtgctc ggcaacgaag agaaggtgcg ggcggcgcaa cgcgaggccg
     7081 gggttgacct cggcggcatg gtgcaggtga tagaccacac gcgcgccgcc gacttcaaca
     7141 aggtggcagc cttctatcac gaactgcgca aggccaaagg cttgatgccc gacgaagcgc
     7201 gcatcgccat tcaagacccg ctctactacg gcaacctgat ggtgcgcgca ggcgcggcgg
     7261 acggctcggt ggcgggcgca accaacacga cggcgcacac cgtgcgcgcc gcgctccact
     7321 gcatcggcgt gcgcgccgga ttcaaactcg tctccagttt cttcgtgatg gctcatcccg
     7381 atccgcagtt cggcgcagag ggcgcgatga tctatgcgga ctgcggcgtc gtcatcgaac
     7441 cgacggcggc ggaactcgcg gagatcgcca tcgcctccgc cgattcgtgc cgcgcgctgc
     7501 tcgaagtcga accgcgcgtg gcgatgctct ccttctcgac gaaggggagc gcgaagcaca
     7561 agttgatcga caaggtggtg gaggcgacgc gaaccgtgcg tgcgcgtatg cccgaactcg
     7621 aaatcgacgg cgagttgcag gccgacgccg cgctcgtgcc tgcggtcgca aactcgaaag
     7681 cgcaggggtc gtccgtcgcc ggacgcgcca acgtgctcat cttcccagac ctacaggcgg
     7741 gcaacatcgc ctacaagttg accgagcgac tcgcgggcgc atcggctatc ggacccatcc
     7801 tgcaaggact cgacaagcct tgcaacgatc tctcgcgcgg ctgcaaagcc gaagacatcg
     7861 ccgatgccgt cgccatcacc gccgtgcagg cgcagagccg gaaaggacag acatgaaaca
     7921 tggtcaagac tttttcgccc cgtccgtccg ccgcgagcgt cgcagacgct gcgagacatt
     7981 gacgcgcgca aggcgcggcg tttgagttcg gccaaggccg cgtcgtcgtg ctgggcgagg
     8041 ccgggatgct ctcggcgcaa ctggcggggg cggacaggcg gcctttcggc atgaatcgcc
     8101 ccggcctcga caatcgccaa ctcgccctca acatcatgcg ctggctctca cgcgtgctgt
     8161 gagtgaattg gcgggcaacg cgcgcactac ctttagagca tcgaacgagt gcaactatta
     8221 gcccggacac gacagttgga gtttatctta tgtacacagg ccgccgtctc ttctggccgt
     8281 tcgccctcgt tctccttttt tgctcggcgg gcacgaaact gcacgcgcaa tcgggtcggg
     8341 tcatcccgtc gccgacgccc gccgaagatc agatcaaagt ctacacggag gaggtgcgcc
     8401 tgcccgtctt tgcgcgcgac gagtacgggc gtttcgaccc gacgctcgaa ctcgacgacc
     8461 tcgtcgtgct cgaagacaac gtgaaacagg aggtgcgcag catccagcgg attccggcga
     8521 gcgtcgtgct cgtgctcgcc acgggcggcg agttgaaccc ggcgcagcgc acgcgcacga
     8581 cgcgcgaggc cgcgctcagt ctcctctcgc acctgcgcgc aggcgactcg gttgccgtcg
     8641 tgcagtttga tcgaaagata aacctgctcc aaccctggac ggttgaccgc gacgcggcgg
     8701 cgcacacgct gcgcacgaaa ctctggggcg cgagcggcgc gcgtcccgcc gaagccttgc
     8761 agcgcgccgc cgaactcttc gcggatcaac ccgtgggcaa ccgtcatctc gtctttgtga
     8821 cggacggcgt ggagacgccg ggcggcggcg cgacgccgga gcagatcacg cgcgtgctgg
     8881 aaggcaacgc cgggatcggc gggcgtacgg cttacgccga agcggtgaag aaattgatgg
     8941 ccgcgcaagt ctccgtgcac atcatcagct acacggagtt tggaaggaat gagactagag
     9001 agaagaagaa gaacgcgccg ctcagcaacg ccgtccccgg cagcgtcaag gcttcgggca
     9061 tcgccaccgc aggcatcgac ccgacgatgc cgccgacgat gaatcgcggc ggggcggttg
     9121 gtccttcaac cggcgcggtc atcactttcg acccggcgat gaagcgtctg cgcaaggctt
     9181 acgagcgcgc gatgaagcgc ggcgaggaga gcatgaagac gctcgcggca gacacgggtg
     9241 ggcggctctg gataccgctc tcgatggaag acctcgcgct gcaaggcagc gaagtggcgc
     9301 gcgagatcgg cacgcagtac gtcgttacct acacgccgag acgcccgctc gccgagagcg
     9361 acgcgacgga gacgcgccgc gtcgtcgtgc tcccgcgtcg caacggactg caactgcgca
     9421 cgcgccgcgt ctacgtcgcc gccgccgcga tgcagaagcc gcccgcgaca aaagcggacg
     9481 cgaaataaga ctggcaagat tcaccggcgc agacgccgag ggggcgcaga gaagattcaa
     9541 ttctctgcgc cccctttgct gctttgcgct gcgtctctgc ggtgaaggct tcccttccgc
     9601 cgaaaacgcg ctccgtttac ttaactttct tcgccagcat ctcgcgcccg tctcgcaggc
     9661 ggacgttaac gtgcgtcacc tgtcctttgt cgtctttgac gaaggtgaga ttaacgttcg
     9721 ggcctgcggc caggaagcga tcattgcctt gcgcgatcaa ctccgctttc ggttgaccga
     9781 ccggcgcgcc gacgagtttg tcgccgtcct tcgtgatgtc gagcacgccc atgcccggca
     9841 cttcgtattg tccggcgtaa gcctcaagcg cgacgggcgc ggttgagggc ggagcctcca
     9901 tgtttttgat gatctggacg gcggctacgt tctgcgggtt gagttcgact gccttcttgt
     9961 agttttgcaa ggccagagcg cgttcgcccg cctgcaaata tgcttcgccg agactgtcat
    10021 aagggttggc ggcctgcggg taggattcga cgttcaactt gaaaatctca atcgcctctt
    10081 tgctcttctt ctggccgagc agttgatagc cgagcgcgtt gagcgcgttt tcgttgaaat
    10141 catagttcgc cgcctgcgtc gttttgagtt cgcgatactg tttgacggcg gcggccacgt
    10201 cctgctccat gatggtcttc atcagcgttt ccgcgatgga ctgtttcggc agttcgaacg
    10261 gctcgttgtg gagaatgttg gtcaggtcgc ggctgatgcg attgaggctt ccgccctgcg
    10321 aggtgttgtc caagaggacg atcaactgtt tgcgctccgg gtagcggacg aggagcgtgt
    10381 tgaagccgtt gatgccgccg ctgtgctgga cggagttgag cacttctttc gattgcgcga
    10441 gcgtgacttt cgtgatgccc cagccgtaag cgtagttgtc gagaaacggc ttgaacatga
    10501 gttccttgct cttggccgag atcagtttgt ccgtgtagag agcctggtcc cacaggaaca
    10561 aatcttcgac ggtcgaatag agagagcctg cggcgtaagg cagagacatg tcgagatagg
    10621 gcgcgttgcg gtagccggtg ggcgtcttct cgtagcccga ggcgcgcttg gcgagcaccg
    10681 cgtcgtaatg atcgtagccg gagtttttca tgccgagcgg gtcgaagacg ttctccttca
    10741 agacctgctc gtaagattta ccgtgaattt tctcgatgat cgcgccgagc aggaagtagc
    10801 cggagttgtt gtaacgaaac ttcgcgcccg gctcgaattc cagatcgccg ctcgcgtatt
    10861 ttttgacaaa ctcgacgggc gtgtaaggat tgcggctcac ctctgccatg aagttcggca
    10921 ggctggtgta gctggggatg ccggacgtgt gcgtcaggag atgatggatg gtcactttgt
    10981 cgcccacgtc cttgcgatag tcgggcaggt actcggagag tttaccgtcg agtttgagct
    11041 tgccctgttc gacgagttgc atgatgagcg tcgcggtgaa ctgcttggtg atggagccga
    11101 ggcgaaactt ggtgtccacc tcgttcggca cgttccattc catgttcgcc agccccagac
    11161 cttttttgta aatgaccttg ccgttttcgg cgacgagcac cgcaccgttg aactgccgca
    11221 gcttgttggc gcgcgtcatg tactcgtcaa tgcgcgcggc cttgtcctgc gcgagcgcgg
    11281 cgggcgactg tgaggccagc gcgacgagca ggacgaacag ccaggcaatt ttcggacgtg
    11341 cgacatgtgt aactctcttc atctctcatt ctcctcaaaa atttacgtgt aggattattg
    11401 cggccagcag gcgacccgtt cgcggcaaac cagcgttcct gatgcaatac gctggcgcgg
    11461 cggcaggagg ttccatcaaa agaagggtgg ccgggaatgt cgagtcttat cgcgtttcgg
    11521 cgtgcgcctg ttgacctgcg aatctttcca gcgcgagcgt gagtcccatg atcagataaa
    11581 agaccatgac cacttccgag tcgccgaggt tgtaatgcac caacccgctc gcgaagaagc
    11641 ctgtgagtcc gccgagcgcg ccgagcacga ggccgcgctc ccgccaatct tccaacccgg
    11701 gcgcacgcca cagacgccac aacatccgcg cgtaaaggaa gacgaccgcc agccagagca
    11761 ggagcgcaaa gatgccccgc tccaaagcaa gctgcaaagg cgtcgagtgc atgtgcccga
    11821 ccggcagacg cccgccgtcg aacagacccc attcccgcca gtgtcgcttc agcgaatcca
    11881 tcccgacgcc gacgagcacg tggcgcggtt cgcgcacgaa caggtcgaag ccctctcgat
    11941 acacggtcgt gcgccaggag atcgaaccgt ctctgcgatc aaaaaatccc acgccgcgct
    12001 tttgctgcaa cacgtacagg ccgagcggta tgacgagcag cgcgccgagc gcgaccgcca
    12061 gcaccgcgcg gcggctgccg acgccgacga gcgagatcaa gacgattgag agcagaaacg
    12121 aaagccacga agcgcgcgtc accgtcaaca gcaacgccgc gccgatccct gcgaccgacg
    12181 ccgcgagcag cgcgccctgc caggagagtt tccggcggtg cgcgacgagg aggccgaagg
    12241 cgagcgagcc gatgagttgc aacacctctg cgtaggtcgt gtagtgaccg taaaaacctg
    12301 acgcgcgttc gtcgcgcccg cgcgtccagc ttgtcaaacc gagttgcgct tccggcgtcg
    12361 cgcccgcgag caacgaaccg cgcgccaact ccgtctcgaa gacccactcg cggcgataca
    12421 cgagcacgcg cgcctgcggc atccccgtca cgttggcggc cagagcgcgc gtgatcgctt
    12481 cgggcgagtt gcacggcgcg cggtcaaggc gcaggagcgt gtcgccttcg ttgatccctg
    12541 cggcgcgcaa gggactgtcc gccgtgagtt tcgctacctt gacgccgcgc ccggcggcgc
    12601 gctggccgaa cgtatagacg acgttgatca tgcacgaagc gacgagcgtg agcgtgagca
    12661 ggcggagcac gcggcgcgag gcgacgttct gtgcgacgag atagacgatg gtgaagaggc
    12721 tcgccgcgcg cagtttgccg atggaaacgt ccgggtcgta ggaagcgagc gcggagatga
    12781 aagtgagtat gaagaagccg agcagcgcgt agtcaacggg cgtgcgttgg agttgcgggc
    12841 gcgggcgcgc ggtgaagcgc agcacccaga gcagcaaccc gacgccccag gcgatttgcg
    12901 tccccgcaat cgagtgcggc gcacagaacg ccagcaggaa caggcacgcg gcgagcgcgc
    12961 gttcaataag cattatcacc acgaagcatc acgggcagcg tgcgcaggat gatcttgagg
    13021 tctaagagga gcgaccagtt ttcgatgtag aagagatcga ggcgcaccat ctcttcgaac
    13081 gagagacggt tgcgacccga aacctgccac aagcccgtca gccccggttt catgtcgagc
    13141 cgcttgcgat gccagagttc gtaggcttcg acttcgtagg tgatcggcgg tcgtggcccg
    13201 acgacgctca tgtcgccgcg caggacgttc aacaactgcg gcaactcgtc gaggctcagg
    13261 cgtctgagca cgcgcccgac gcgtgtgacg cgcgggtcgg tgcgcagttt gtagatgggt
    13321 cgctcgtcgt cgcccagatt cgtctccgcg cgcccggcga tgtagttgcg ttgataatcg
    13381 cggtgcgcgg cgtcgtcgga accggactgc atggtgcgga atttgaggaa gaggaagatg
    13441 cgcccgtcca tcccgacgcg ctcctgcttg taaaagacgc cgcccttcga atccagcttg
    13501 atgagcacgg cgatgacgag ccagagcggc gcaagcagcg cgagcgcaaa cgaagcgacg
    13561 agaatgtcca tcgtgcgttt gaggatgcgc gcgctgttgg agagcggttc gcggaagagt
    13621 tggatcatcg gcagcgcacc gatctggtcg atctcggtct tgcgcggcag gcagttgaac
    13681 aggctcggcg cgatgcgaaa ctcgacgccg cgcagacgcc cgacgcgcat catcacgtcg
    13741 aacaacaggt cgccggagac gcccgcgtcc gccacgatca cttcgttggc cgccgtctcg
    13801 cggatcgtct cggccagatc gcgcacgctg gcgataacgg gcacgccctc gaaagtctcc
    13861 ggcacgcttg cttgtatcgc gccgttctcc accacgccga tcactcgata gcccaactct
    13921 ggccgctccc gcatctcacg gatgcacaga ctcgcctccg cgccgcgccc gacgacgagc
    13981 gtgggaatca ggtttacgcc ccgccgacgc acggcggcct gcgccgcgcg cacgcccatg
    14041 cgcaacgcgc cgaacgccgc gagcgcaaac gcgaaatcaa gcacgaagac ggcgcgcgaa
    14101 taggagaagg cgcgaaaagc cgtgccgccg cgatagagga aagcgaccgc gatgatcagc
    14161 agcgacgcca ccgccgtcgc cttgaagacg cgcagagcgt cgtccacgta agaga
//
LOCUS       DASWNS010000398         5962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_15995, whole genome shotgun sequence.
ACCESSION   DASWNS010000398 DASWNS010000000
VERSION     DASWNS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15995"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1945
                     /locus_tag="VGB76_13955"
     CDS             197..1945
                     /locus_tag="VGB76_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100049.1"
                     /translation="MKSWPQRILLSIVVLAVLLAAWVWWNRPETVDMAAYVPAESLIY
                     LETNSLPEIVGGLTSTDAWRALAPPAGLSPNVGRIGWLSRLSAWSGVGSAETVVFSRA
                     QVAVAVLGFEVTEESSTVRDIKPRIALVAETHTGAARVQAAVTKLVGNYARRKYGEPA
                     ILRDEVDASTLITWTTPDGKRKIVAAINESVAIVGNDEEAVRACLSVRRGERASLAND
                     AQLAEMRLRMKADGALAFGYVSAAGVGRLLEMAAVAYGGQVASDPKMQSAAAILIPQL
                     ASRILGSVAWSSAIAEGTVVDNYYLQLQNELASRLRTPLATSGADSMRAGDLLPADTN
                     QLTIYNNRDPEAAWRGLNAAISSQLEITIAPLVGRFLAESLQPFGIESPHDFLRAAGP
                     QIATARLDSEGSALVLLAPVRERETLQGLVRKRLGAGAKSERVGEAEMLVATDEERGA
                     ASFVGDYLIMGGVEEVRRCLQERAASQTLGSVRAFQQSSRPADSNEAANVVTYTNDYE
                     PARRFISFLGRQNGVRNSPPDAAALQRSLSALPYAFSETRLVEGGFEKKTRSAFGQFG
                     SILVQLATDVETDRGK"
     gene            complement(2106..3473)
                     /locus_tag="VGB76_13960"
     CDS             complement(2106..3473)
                     /locus_tag="VGB76_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100050.1"
                     /translation="MTSLAERTTLQGTPEAFAPPLRRASDNRASVDDSSLNELHIWQL
                     ALRSFFNLRNHSLSDAERAEIVAHDFSPELKIVNQVLRRSLLLSLNCTSGATHASFEM
                     EETAPFEDISLHAVPRGATTQEDNSPLVPLINTLSDLCRMCESVPSVKVDFQVWSSVE
                     QMVRRELEHSPFIEQLERRLRTATLLRQYPELHKLTERMTPDTLAADMSAIFSQIMQM
                     LEQLRLVETLLRQDAPLKQSLAIFTLVHEETRALVDFIEKRAIHTEDIDGAVFDALDC
                     TGYAIGVELTRVFGRELLGLASLRQSPAIYTKVENAHGLLRDSFQQSLVSLAQVFDAT
                     FNGAHFFQSFRSRLAQSLVLRRDLWRLRQLVLQAEQDRDTRPLVPLIERLDAFREGSL
                     RFLMYKDWETYERFVAEVLAARGAVELAPVLHRFGTYLEALFGQINMRIVLADHPFDY
                     PALVE"
     gene            3657..4277
                     /locus_tag="VGB76_13965"
     CDS             3657..4277
                     /locus_tag="VGB76_13965"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:NF011397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HEY0100051.1"
                     /translation="MTPTPHPELLIATHNTGKIRELSELLSNLPLRLRRLSEFPDIEE
                     VEETGDTFAANAELKARLYSRQTNLWTLADDSGLEVDALGGAPGVFSARYGGANATDA
                     ERNLRLLAELSRTDDPQRNARFTCTIALYEPKAERLEIFTGTCEGRISRHPRGNRGFG
                     YDPLFIPTGYSDTFGELPDELKQQISHRARALNAVRAYLHGHFSRP"
     gene            4360..4433
                     /locus_tag="VGB76_13970"
     tRNA            4360..4433
                     /locus_tag="VGB76_13970"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4394..4396,aa:Pro,seq:tgg)
     gene            complement(4443..5564)
                     /locus_tag="VGB76_13975"
     CDS             complement(4443..5564)
                     /locus_tag="VGB76_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0100052.1"
                     /translation="MSVFTRSCGSKGCRKKHWHYAFAIRNVRYRGAIPEARTKFEAEQ
                     AEVTIKGEVFEGRYGRPSGGHDFVKFVEEIFVPSARANNRSWDKSGFYTLPVLCEWFK
                     GKSFAQISPLMIERFKRERRDSVTKKGTTRQPATINRELQLLSTIFTLAVKHKVAATN
                     PCKEVALLPENNRRTRYLLDEEEARLMAVLVGPRAHLKDLVVVAIGTGMRRGDQLSLR
                     WEKVDFQRNVIYVPNSKTGKDYAVPMDDDVRDVLLRLRREAKGREFVFVNPETGGPYA
                     DMKKAFGTACRLAGIDNLHWHDLRHTFGTRLAESGCSEATIAGMMGHSDPGTTRRYTH
                     ATDRAKHAAVEAVKAGRASACHSPATDEKRRPKLAAVNS"
     gene            complement(5632..>5962)
                     /locus_tag="VGB76_13980"
     CDS             complement(5632..>5962)
                     /locus_tag="VGB76_13980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0100053.1"
                     /translation="FLGKVANSFENRKGVMSAIGKNESAAERIGDVEASKAHLKLMPR
                     APVKLPPGVPQYLNVEEVATMLQLRPKTIYKMVSQRRIPFRKAGHHLLFDQREIEAWT
                     KAGADKQ"
BASE COUNT         1203 a         1749 c         1739 g         1271 t
ORIGIN      
        1 ggtgtcaaca acaatttact ttgtttaact catagatttt acaaaccttg ttccatcatt
       61 ttgacctgcc cgcgcctatt tctggaaaaa gcctgcgcca cttccaattt cgctgacgag
      121 cgatatttct ccggctgcga gcggttgaaa gcgtgggttc ttgcttcgtt cgggcggcga
      181 aaggtaggat gctggaatga agagttggcc gcaaaggata ctcctgtcaa ttgtcgtgct
      241 cgccgttctg ctcgccgcgt gggtctggtg gaatcgtccc gaaacggttg acatggcggc
      301 ctatgtgccc gccgaatcgc tcatctatct cgaaacaaac agcctgcccg aaatcgtcgg
      361 cggcctgact tcgaccgatg cctggcgtgc gctcgcgccg cccgccggtt tgagtcccaa
      421 tgtcggacgc atcggatggc tgagccgtct atcggcgtgg agcggggtcg gctcggcgga
      481 gacggttgtt ttttcgcgcg cgcaggtggc ggtggcggtg ctcggatttg aagtgacgga
      541 agaatcgagc acggtgcgcg acatcaaacc gcgcatcgcg ctcgtcgccg aaacgcacac
      601 gggcgcggcg cgcgtgcagg ccgctgtcac caaattggtc ggtaattacg cgcgccgcaa
      661 gtacggcgag ccggcgatcc tgcgcgatga agtagacgct tcgaccctca tcacctggac
      721 gacgccggac gggaagcgaa agatcgtcgc ggcgatcaac gagagcgtgg caatcgtcgg
      781 aaacgacgaa gaggcggtgc gcgcctgcct ctctgtgcgc aggggcgaac gggcgtcgct
      841 ggcgaacgat gcgcaactgg cggagatgcg tctgcgcatg aaggcagacg gcgcgctcgc
      901 tttcggttac gtgtcggcag ccggggtcgg gaggttgttg gagatggccg cagtcgctta
      961 cgggggtcag gttgcgtccg atcctaagat gcagagcgcg gcggcgatcc tgatcccgca
     1021 gttagccagc cgcatactcg gcagcgtagc ctggagttcg gcaatcgccg aaggcacggt
     1081 cgtcgataat tattatctcc aactgcaaaa cgaactcgcc tcgcgcctgc gcacgccgct
     1141 cgccacttcc ggcgcagaca gcatgcgcgc aggcgacttg ttacccgccg atacgaatca
     1201 actcaccatc tacaacaacc gcgaccctga ggccgcgtgg cgcggcttga acgcggctat
     1261 ctcttcacaa ctggagatca ccatcgcgcc gctcgtcggg cgtttcctcg ccgaatcgct
     1321 acagcctttc gggatcgagt cgccccacga tttcctgcgc gccgccggac cacagatcgc
     1381 cacggctcgc ctcgatagcg aaggctccgc gcttgtgctt ctcgcccccg tccgcgaacg
     1441 cgaaactttg caggggctgg tacgcaaacg tctgggggcg ggcgcgaaaa gcgagcgcgt
     1501 cggcgaagcg gagatgttgg tcgcaacgga tgaagaacgc ggggcggcga gttttgtcgg
     1561 cgactacctg atcatggggg gcgtagaaga agtgcgtcgt tgcctgcaag agcgcgccgc
     1621 gtcgcagacg ctcggctcgg tcagagcatt tcagcagtcc tcgcgtccag cggattcaaa
     1681 cgaggcggcc aacgtggtca cctatacaaa cgattacgag cctgcacgcc gcttcatctc
     1741 ttttctcggc aggcaaaacg gcgtgcgcaa ctccccgccc gacgctgccg ctttgcagcg
     1801 ttccctctcc gcgctgcctt acgcgttcag cgagacgcgc ctcgtcgaag gaggttttga
     1861 aaaaaagacc cgctctgcgt tcgggcaatt cggctctatt ctcgtgcaac tggcgaccga
     1921 cgttgaaact gatcgtggga agtgaccgga aaatttacgc gctcttccgg tttagattca
     1981 gctaaaagat tgaccgccgc agaagcagag atgaacgccg aagggggcgc agtgaaagat
     2041 tcaactcttc tctgcgcccc cttcggcggc ctggctcgcg tgtctacggc gttgagtcga
     2101 aaggtttatt caactaaggc cggatagtcg aagggatggt cggcgagcac gatgcgcatg
     2161 ttgatctggc cgaacaatgc ttcgagatag gtgccgaagc ggtggagcac gggcgcgagt
     2221 tcgaccgcgc cgcgtgcggc caacacctcc gccacgaatc tctcgtaagt ctcccaatcc
     2281 ttatacatca ggaagcgcaa acttccctcg cggaaggcgt cgagacgctc aatcagcggc
     2341 actagcggtc gggtgtcgcg gtcttgctcc gcctgtagca cgagttgacg cagcctccac
     2401 aggtcgcggc gcaacacgag cgactgtgca agtctcgaac ggaaagattg gaaaaagtgc
     2461 gcgccattga aggtcgcatc gaagacttgc gcgagggaga cgagcgattg ctggaaacta
     2521 tctcgtaaca gtccgtgcgc attttcgact ttcgtgtaga tggccggcga ttgtcgcaga
     2581 gaggcaaggc cgaggagttc ccggccaaag acgcgggtta actccacgcc gatagcgtag
     2641 cccgtgcaat cgagtgcgtc gaagaccgcg ccgtcgatat cctccgtgtg gatcgcgcgt
     2701 ttctcgataa agtctacgag cgcgcgtgtc tcttcatgta cgagcgtgaa gatggcgaga
     2761 ctctgtttta acggcgcatc ctgccgcagc aacgtctcga cgagacggag ttgttcgagc
     2821 atctgcatga tctgggaaaa gatcgcagac atatccgccg cgagcgtgtc gggcgtcata
     2881 cgctcggtca gtttatgtag ttcgggatac tggcgcagca aggttgcggt gcgcaggcgg
     2941 cgttcgagtt gctcgataaa cggcgagtgt tccagttccc gcctcaccat ctgctccacg
     3001 ctgctccaaa cctgaaaatc aaccttgacc gacggcacgc tctcgcacat gcgacacaga
     3061 tcgctcaagg tgttaatgag tgggacgagc ggagagttat cctcctgagt tgtcgcgccg
     3121 cgcgggactg cgtgcagaga gatgtcttca aatggtgccg tctcctccat ctcgaaagag
     3181 gcgtgcgtcg cgccggaagt gcaattcaaa ctgagcaaca ggctgcgtct gagcacctga
     3241 ttgacgatct tcaattcggg cgagaaatca tgcgcgacga tctctgctct ctccgcgtcg
     3301 ctcagagaat gattgcgcag gttgaagaag ctgcgcaggg ctagctgcca gatgtgtagt
     3361 tcgttcaaac tcgaatcgtc aacgctggcg cggttatcgc tcgcacgccg gagaggtggc
     3421 gcgaatgcct ctggcgtgcc ctggagagtt gtgcgctcgg ctaaagatgt cattatgttc
     3481 aaagctgtaa ccatgtgaaa attctcgtga agattcttcc ggcgcggttc gtgtgagtgt
     3541 gtgcgccgcc ttttattgtt agcatgaatt ccatcagctt ggcaaaaaag ttttttagta
     3601 actattattg agagccgtaa atgcctccat tcgaacattt caactcctaa aaatttatga
     3661 ccccgacgcc gcatcccgaa ctgcttatcg caacccacaa cacgggcaag attcgtgaac
     3721 tgagcgaact gctatcaaat ctaccgctgc ggctgcgccg tctgtccgag tttccagaca
     3781 tagaggaggt ggaagaaaca ggagacacct tcgcggcaaa cgcggaactc aaagcgcgcc
     3841 tttacagccg ccagacgaat ctctggacgc tggccgacga ttcgggtctg gaagttgacg
     3901 cgctcggagg cgcgcccggc gtcttctccg cacgctacgg cggcgcgaac gccacggacg
     3961 cagagcgcaa tctacgtctg ctcgcagaac tcagccggac agacgacccg caacgcaacg
     4021 cacgcttcac ctgcacgatt gccctctacg agccaaaagc agagaggttg gaaattttca
     4081 caggcacttg tgaaggtcgc atctcccgcc atccgcgcgg aaacagaggc ttcggttacg
     4141 acccgttgtt catccccaca ggctattcag acactttcgg cgaattgccc gatgagttga
     4201 agcagcagat cagccaccgc gcgcgcgcgc tcaacgcagt tcgcgcttac ttgcacggcc
     4261 atttttcccg gccatagctt gacccgccgc ccttcgcact ggcagaatgc gctgcattcc
     4321 gatttattta atatttcgtg agaagtcgga agatttacac ggggcgtagc acagcctggt
     4381 agtgcgctcg gtttgggacc gagaggtcga gtgttcgaat cactccgccc cgactattaa
     4441 tttcaagagt ttacggcggc cagcttcggt cgccgttttt cgtctgtggc aggactgtgg
     4501 caggctgaag cgcgccccgc tttgacagcc tccaccgcgg cgtgtttagc ccggtccgtc
     4561 gcgtgtgtgt agcgcctcgt cgtgcccgga tccgagtgcc ccatcatccc tgcaatcgtc
     4621 gcctccgagc aacccgactc ggccagcctg gtgccgaacg tgtggcgcag atcgtgccag
     4681 tgtaggttat caatcccggc gagccggcag gccgttccga atgccttctt catgtccgcg
     4741 taaggccctc cggtttccgg attcacgaac acgaattccc tgccctttgc ctctcgccgg
     4801 agccggagta gaacgtcgcg cacgtcatca tccatcggca ccgcgtaatc cttccctgtc
     4861 ttggagttag gcacgtaaat cacgttgcgc tgaaagtcaa ctttctccca tcgtaaggag
     4921 agctgatcgc cgcgcctcat ccccgtgccg atggccacca ctaccaagtc tttcaggtgc
     4981 gccctcgggc cgacgagcac tgccatgagt cgcgcttcct cttcatcgag caggtagcgt
     5041 gtgcgccggt tattctccgg taacaatgcc acctctttgc acgggttggt cgcggccacc
     5101 ttgtgtttga ccgcgagggt aaagatggtt gagagcaact gcagttcccg gttgatggtt
     5161 gcgggctggc gggtagttcc ctttttggtg acggaatccc gcctctcacg tttgaacctt
     5221 tcaatcatca aaggggaaat ctgcgcgaag ctcttaccct tgaaccactc acagagcaca
     5281 ggcagcgtat agaagccgct tttgtcccag gagcgattat tagcgcgcgc cgaaggaacg
     5341 aagatctcct cgacgaactt aacgaagtca tggcctccgg atggcctacc atagcgccct
     5401 tcgaagactt cccccttgat cgtaacctca gcctgttcgg cctcgaactt ggtgcgcgcc
     5461 tcgggaatag ctccccggta gcgcacgttg cggatggcga aggcgtagtg ccaatgcttc
     5521 ttccgacagc ctttgcttcc acatgaacgt gtgaaaaccg acatgatcgt cttcctttcc
     5581 cgagctgcag ccacgggtga gccgcatcct acttgatcgg ccacagtgag gtcactgttt
     5641 atctgcgccc gcttttgtcc aggcttcgat ctctcgttga tcgaaaagca gatgatgccc
     5701 cgccttacgg aatgggattc gccgctggct taccatcttg taaatagttt tgggacggag
     5761 ttggagcata gtggccactt cttcgacgtt gaggtactgc ggcacgccag gcgggagttt
     5821 aactggcgca cgtggcataa gttttaagtg agcttttgaa gcctccacat cgccaatacg
     5881 ctccgcggct gattcattct ttccgatagc actcataact ccctttcggt tttcaaagct
     5941 gttagcgact ttgcctaaaa aa
//
LOCUS       DASWNS010000399        15296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_144247, whole genome shotgun sequence.
ACCESSION   DASWNS010000399 DASWNS010000000
VERSION     DASWNS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15296
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144247"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..4034)
                     /locus_tag="VGB76_13985"
     CDS             complement(204..4034)
                     /locus_tag="VGB76_13985"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0100054.1"
                     /translation="MHYLIRSRSALAFVCAVACLFFFYATSTPLRRVIAHEERRAAER
                     RQSLPQAQSDVPADLRAAIEKMRADEGREVRPQANDAGAQYPLTVDPFMQQQRLHASD
                     AATGDDFGYSIAISGDTAIIGAPLDDNSGGVDAGSAYIFTRSAGVWSEQQRLQASDAA
                     PLDGLFNTSVAISGDTAILGVSEDKNSGGERAGSVYVFTRSAGVWSEQQRLQASNAQF
                     SSHFGSAISISGETMLIGAASTYFNGVESGTAYIFTRSAGVWRERQQLQASDAALNDD
                     FGASVALSGDTAIIGAPTDKNSAGFAGSAYVFTRSADVWDEQQRLQASDASSGDHFGQ
                     SVSISDESVLIGAYFDDNSGGVDAGSAYIFTRSAGVWSEQQRLQASDAAYADLFGNAV
                     SISGDVAVIGSFFDDTLFVGGSGGTNAGSAYIFTRSGGVWSERQRLEALNGDDYSPFG
                     VSVVISGDTVMVGAAPDSFNGGPFPGSVYVYNMGPDRWGEQQQLQSPESASGDYFGYS
                     VAISGDTAVVVATGEDDDQGVPIGAAYFFNRCAGVWSLQQRLHVSADNGHSRGLSFQS
                     VAISGNTAIVGAEGQDENAGAAYVLTRSRGVWSQQQRLKASDAVAQDRFGVSVSISGD
                     TAIIGAYADDNSGAFNAGSAYVFTRSAGLWSEQQRLQASDAAGLDYFGWSVAVSGDTA
                     LVGAERNDNSNGADAGAAYLFTRSGGVWGQQQKLLPITSPGYGHNFGYSVAISGETAI
                     IGAYGDHLDNVLRAGAAYVFTKRGVNWHQQQRLRASVPATDDFFGGSVAISGETVIVG
                     ALIDDNSGGVNAGSAYVFTRSGGIWSEQRRLQASDAAAHAIFGSSVSISGDTAIVGAF
                     AHSGPAAPGAAYIFGTPPPASLVLISEFRLAGPGVGGTSAGTDEFVELYNNTDADITV
                     GTTDGSAGWTLRSSDGTLNFTIPNGDVIPARGHYLLTNSDGYSLAPYPTSQHGCAGGN
                     CAHGNRTFASDTPENTGLALFRTANAANFTLANRLDAAGAVTEADALYREGEGYTPVS
                     GSTATVQHSLVRNLLSGTPRDCDHRDFQLVATDGAATLPGAVLGAPAPENTNSPVQRN
                     ASLKASLLDTMQPSSVAPNRVRSGAADPSEPANSTRGTLKIRRKFTNATGAAVTRLRF
                     RVADITTTPRRNSATADLRVLSGAGGVNVSLSNGASVPLIALTREEPPAQSAHGGGLN
                     TTLSATLAQAIPHKASIYVEFNLGVIEDGNFSFHVNVEALTNPPTDSPANSKPDALRK
                     ASKSMKAGTPPATP"
     gene            4415..5182
                     /locus_tag="VGB76_13990"
     CDS             4415..5182
                     /locus_tag="VGB76_13990"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEY0100055.1"
                     /translation="MKLVVLGSGTSVPHPGRASAAHWLETAGGSTLLLDISAASVHRM
                     AQEGLDWVNLDAVWISHFHLDHLGGLFPFLFGTKHAPATQTRRKPLHIYAPHGLKKLF
                     AAFDAAGDYNLLKQPFPVEIREVAPRAEFEILPGLRAETFSTPHTDESLAIKLTDRDE
                     ASLAFTSDTGYTDALAAFARNVSLFLMECSFFRDKPVELHLELAEAMRLAESSGARRV
                     MLSHLYPEWDNYNLVAEAKRLWRGETIEARDGLRLEI"
     gene            5276..6205
                     /locus_tag="VGB76_13995"
     CDS             5276..6205
                     /locus_tag="VGB76_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0100056.1"
                     /translation="MKSRLIINPVSGTDSAPDHLQTINERLRAAFGEMDIVMTVGAGD
                     ASAAAEAAAASGVYDQLFVAGGDGTLNEVVNGVARVAGGFERITFGLVPLGTGNDFAS
                     ALGLPEEIEAAIEILLRGRTVMCDVGALGDRRFVNVSAGGFIAEVSDAVNPQLKTVAG
                     KLAYLVGGAQVLFDYEPVGAQVRSTGEDGRVSERKLDVQMFAVCNSRMVGGGRLIAPH
                     AYVDDGLLDVCLVEAMPVIEFIGLLTSVSAGEHLTDERVSYFRARSLELSFARTIKVN
                     TDGEVLETDHCRYTLEPRAARFLAGDAPFAAEQ"
     gene            complement(6430..8550)
                     /locus_tag="VGB76_14000"
     CDS             complement(6430..8550)
                     /locus_tag="VGB76_14000"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0100057.1"
                     /translation="MNHYSTKKFCAHALLLALLLAARDAPAQQTNTQPSTPVTSAAAR
                     QHASPQVQLADVPLMGTLMVASQEAASKPARTGAEPGAQARTLPAQQQQTAQPSPQAT
                     PQTTTQTPVTPTPTPTPTTNATTDATTAEAQAQTQPTPSNAQAETALPVPAIAPDYRA
                     PATAPLPALERVGVNMAEQRPLALREAIELALKNGKDIEVARTNVRAAEFDLTAARGA
                     YDPRFNTTTFYERNKTPVASFLSGGEGGAVTSSGLGGTMRFEGLTPRGGGNYRFDFSS
                     SRQTTNSLFNALNPQYPTAFSFTYAQPLLRGFRFDATRRQIEIAKKNLSLTDAQFRQR
                     AIETITSVQRAYWDLVFTLRNLQVQRDSARDARTQLEHNRRLVAEGQLAPIDIVAAEA
                     QVANFEQNVYVALDEVGRAENALKNLIAENRQSEVWSVALVPTDMVDVQPPSVALEDA
                     MRSALATRPELQQSDVVREINALDERLAREATKPQVDLVGSYGAVGLAGAYDPATSVN
                     PFSASSDATRERINQLISLAPPLPDGTRIQPLPPVAAGAIPDTLVGGYGQSIANLGAN
                     RFNNFRVGVQLNLPLRNRTAEAQLGRTLVERDRIRVQREQLEQLIQVEVRNALQVTRT
                     AESRLRAAAAQRSATEQQYASEQRKFEAGQSTFFLVLERQNAFATARGNELRAQTELN
                     KAVAELQRATGNALEANQVQVRVR"
     gene            complement(8586..11909)
                     /locus_tag="VGB76_14005"
     CDS             complement(8586..11909)
                     /locus_tag="VGB76_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0100058.1"
                     /translation="MQKLAEICVRRPVFATMLILSLTVVGIFSFFSLGVDLFPKIDFP
                     TITISVVNPGASPREIETEITDKVEAAVNTISGIDELRSTSVEGVSQVFVQFLLDKNA
                     DIAAQEIRDKVNLITSDLPETAEEPVIQKLDTDAAPVLRIAVSAPRSLRDVTEIADKQ
                     IKERIESINGVGNVEIVGGSRREIHLKLDPDKMRAFNVTVPEVAAAVRAQNLEVPGGR
                     VDEGSRELTVRTLGRIMNPSDFNHIAISTRGDYPVKLSDIGYAEDSSEELRTSARLNG
                     EPAVTLIVAKQSGTNTVAVADEIKARLEEIKPTLPSNIRMEVIGDSSIFIKAAVESIQ
                     THLIEGSILAAIVVFVFLWNFRSTLIAGLAIPTSIVATFGLMAALGYTLNMITMLALT
                     LMVGIVIDDAIVVLENIYRFIEEKGMPPFQAAIEGTREIGMAVMATTLSLLAVFLPVG
                     FMGGIVGRFMSSFGLTASFAVAVSLIVSFTLTPMLAARLIKRKKPQPVSPGVGSEPSE
                     GEASSLLANESRVVAGEPEKAEPESATQLDAEVLPSETHVDDGGREQKKSHGSKDGRF
                     YRPVDRAYTAMLKWSMAHRWAIVGLCVLVIVSIVPLFMFVGKNFLPVDDQSQFEINMR
                     APEGSTLANTSTIVERIAADVRTLPGVTDTLVTIGGDRQQLVNSANIYVKLSDLSERE
                     LSQTDLMVRARELLKKYPPELRTNIGQVQAISGGGQRNADIQYMIGGPDLQTLTKYSE
                     DLLEKMKTIPNVVDIDSSLISGKPELRVIIDRARAADLGVRVGDIAQSLNTMVAGQKV
                     STFNQGTDQYDVRLRAIGAARASKEELSRLFAPSAKLGLVSLDNLVRIEEGSGPSAID
                     RLNRQRQVTLTANVAPGGSQSAVLAELDNAVAAMNFESGYNTGLVGRSKELGRAGAYF
                     LLAITLSFIFMYMVLAAQFESFIHPVTILLTLPLAIPFGILSLLLTGQTVNIFSGLGL
                     LLLFGVVKKNAILQIDHTNELRAKGMDRHDAIIQANRDRLRPILMTTIALVAGMTPLV
                     ISSGPGSGTNRSIGVLVVGGQSLCLLLTLLAVPVFYSLFDDIGRSKVFGRVGSGIGGA
                     FAWARRRTATAAGAIFNKF"
     gene            complement(11991..13250)
                     /locus_tag="VGB76_14010"
     CDS             complement(11991..13250)
                     /locus_tag="VGB76_14010"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0100059.1"
                     /translation="MSSRNKFTLPTVILLLAALSLFASGCGRSSADAKNKQAAANATP
                     EVVQVSTAAAITRELPRYVEATGSLAADQQTDVAPTVAGRVVAVGVDLGSYVQRGSLL
                     VRLDDADARLRLTQLQAQAQQAQSAVRQAEARIGLRPGQAFDPNRVAEVGAARVALEL
                     AEKQLRRFERLIESGDVSRADYDRQKSQRDQLQQQYEAALQAARQNYAGIATARAAAN
                     AAEAQVAQARKAVSDVAVYAPISGYVADRPADLGEYVSPTSKVATIVRTNPLRMRIDI
                     PEQAISTIQPGQTVSITTSAYPDRSFAGRIARISPNIVATSRTLTVEAEVENGEGLLK
                     PGQFATVRISQPKGDAAVLVPSTAVRTEQDVSRVFVIRDGRVQERVVQLGQVEGELVE
                     IKTGISVDELVATSNVEMLKDGIAVRQ"
     gene            complement(13337..14152)
                     /locus_tag="VGB76_14015"
     CDS             complement(13337..14152)
                     /locus_tag="VGB76_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0100060.1"
                     /translation="MSRVLETKGATEAVAAGTATGANSSGRMAGEDRRRQILQVAMSL
                     FSKRGFRGTTTKEIAHAAGVSEAMVFRHFANKQELYSAIIDHKACAANFQQPCDIVPD
                     ALARKDDRAVFSGIALAMMRHHEHDTEFLRLLTQSALEGHELADMFWDRNVRHMYDFL
                     GSYIRERQREGAMRNIDPNIVVRCFLGMVIHHSLNNILWDTRRRILSIPNERAAEEFT
                     EILLRGIAAHATTHNPPPASRNSNGSSSSNANGSGERAKHGSAANPTRAKKKR"
     gene            14524..14997
                     /locus_tag="VGB76_14020"
     CDS             14524..14997
                     /locus_tag="VGB76_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100061.1"
                     /translation="MSQNFGNNDPLNTGGNTGTTGGTGLAGTGRTDEGTTRDTSTGGS
                     GIGGGSGVGGGLSTTGGATGGTSTGTATAREGIAGTAQEYGQKLADAASTAKDYLGDK
                     ISVAGDKFKDLQNVDYRQAAEQAKNYAREKPGQALLISAAAGFLIGLLLKSSNRR"
     gene            15109..>15296
                     /locus_tag="VGB76_14025"
     CDS             15109..>15296
                     /locus_tag="VGB76_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100062.1"
                     /translation="MAELERRAAGGSAATAPATTAAQNAQGLDIEGLPAMLGRLGDNV
                     MTLVDAKLGLAKVELKEEA"
BASE COUNT         2703 a         4726 c         4920 g         2947 t
ORIGIN      
        1 gcgtcgtccg ggtgcgagaa gacggccagc gcgtctaagc ttgtgtgttc tgtgctcatg
       61 tttctggaag ggtgttgttc cctgcagaca ggtttaaccg aagccgcgcc cgccggtcaa
      121 gcaaaaggct ccgacgttgc cgccggagcc ttgcgccgtt caaccggagt tgattgagag
      181 caacgtacgc cgcgcgccgt ttctcagggg gtcgcaggcg gcgtcccggc cttcattgat
      241 ttcgaagcct tcctcagtgc gtccggcttt gagttcgcgg gcgagtctgt cggcgggttg
      301 gtcaacgcct cgacgttgac atgaaagctg aagttgccgt cttcgataac gcctagattg
      361 aactccacgt agatgctcgc tttgtgcggg atggcttgcg cgagcgtggc cgagagcgtg
      421 gtgttgaggc cgccgccgtg cgcagactgc gcgggcggtt cttctctggt caaggcgatg
      481 agcggcacgc tcgcgccgtt gctcaagctc acgttcacgc cgcccgcgcc ggagagcaca
      541 cgcaggtctg ccgtcgcgct gttgcgtctg ggcgtggtcg tgatgtctgc gacgcggaaa
      601 cgcaggcggg tcacagccgc gccggtcgcg ttggtgaact tgcgcctgat cttcaaagtg
      661 ccgcgcgtgc tgttggccgg ttcggacggg tctgccgcac cgctcctgac gcggttcggc
      721 gcaaccgaag agggctgcat cgtatccaga agcgaggctt tgagcgaggc gttgcgctgg
      781 acgggcgagt tggtgttctc aggggcgggc gcgccgagca ccgcgccggg cagtgtggcc
      841 gcgccgtccg tggcgacgag ttggaagtcc ctgtggtcgc agtcgcgggg cgtgccggag
      901 agcaggttgc gcacgaggct gtgctggact gtcgccgtgc tgccgctgac gggagtgtag
      961 ccctcgccct cccggtagag cgcatcggcc tctgtgaccg cacctgcggc gtcgaggcgg
     1021 ttggcgagtg tgaagttggc cgcattcgcc gtgcggaaga gagcgaggcc ggtattctcc
     1081 ggcgtgtcgg aggcgaaggt gcgattgccg tgggcgcagt tgccgcccgc gcaaccgtgc
     1141 tgcgaggtcg gatagggagc gagcgagtat ccgtcggagt tggtcagcag gtagtggccg
     1201 cgcgcaggga tcacgtcgcc gttcgggatg gtgaaattga gcgtgccgtc ggaggagcgc
     1261 agcgtccagc ccgccgaacc gtccgtcgta ccgacggtga tatctgcgtc ggtgttgtta
     1321 taaagttcga cgaactcgtc tgtgcccgcg ctcgtgccgc caacgccggg acccgcgagg
     1381 cgaaactcgc tgatgagaac aagacttgcc ggcggcgggg tgccgaaaat ataggccgcg
     1441 ccgggggctg ccggaccgct gtgcgcgaac gcaccgacaa tggccgtgtc accgctgatt
     1501 gaaaccgagg agccgaagat cgcgtgagcg gcggcgtctg atgcctgcaa tcgccgctgc
     1561 tcgctccaga tgccgccgct ccgtgtgaag acgtaggccg agccggcgtt aaccccgccg
     1621 ctgttgtcat ctattaatgc gccgacgatg accgtctcgc cgctgatggc gaccgagccg
     1681 ccgaagaagt cgtcggtggc cgggactgat gcccgcaaac gctgttgctg atgccagttg
     1741 acaccgcgtt tggtgaaaac gtaagccgcg cctgccctta acacattgtc gaggtgatct
     1801 ccatatgcgc cgatgatggc cgtctcgccg ctgatggcaa ccgagtagcc gaagttgtgc
     1861 ccgtagccgg gtgaggttat cggcagcaac ttctgttgct gcccccacac accgccgctc
     1921 cgtgtgaaga ggtaagccgc accggcatct gctccgttgc tgttgtcatt tcgttctgcg
     1981 ccgacgagag ccgtgtcgcc gctgacggcg accgaccaac cgaagtagtc aaggccagcg
     2041 gcatccgatg cctgcaaacg ctgctgttcg ctccaaaggc ctgcgctccg tgtgaagacg
     2101 taggccgagc cggcgttaaa cgcaccgctg ttgtcatcgg cgtatgcgcc gatgatggcc
     2161 gtgtcaccgc tgattgaaac cgagacgccg aagcggtctt gagcgacggc gtcggaagcc
     2221 ttcagacgtt gttgctggct ccacacgcct ctgcttctgg ttaaaacgta ggccgcgcct
     2281 gcattttcgt cctgcccctc cgcgccgaca atcgccgtgt tgccgctgat ggcgaccgat
     2341 tgaaaggaga ggccccgaga atgtccgttg tcggcggaca catgcaaacg ctgctgtaaa
     2401 ctccagacgc ctgcgcatct gttgaagaag taagccgcgc ctatgggcac gccctgatcg
     2461 tcgtcttctc cggttgcgac gacaaccgcc gtgtcgccgc tgatggcgac cgagtagccg
     2521 aagtagtcac cggatgcgct ttccggcgac tgcaactgct gctgctcgcc ccacctgtct
     2581 ggacccatgt tgtagacgta gacagagcct gggaacgggc cgccgttaaa ggagtccggg
     2641 gcggcaccga ccatcaccgt atcgccgctg atgacgaccg agacgccgaa gggggaataa
     2701 tcgtcgccat tcaatgcttc caaccgctgc cgctcgctcc acactccccc gctgcgggtg
     2761 aagatgtagg ccgagcctgc gttcgtgccg ccgctaccgc cgacgaataa tgtgtcatcg
     2821 aagaatgacc cgatgaccgc cacgtcgcca ctaatagaga cagcgttgcc gaataagtcg
     2881 gcgtaggcag catccgacgc ctgcaaacgc tgctgctcgc tccacacgcc cgcgcttctg
     2941 gtaaaaatgt aggccgaacc cgcatcaact cctccgctgt tgtcatcgaa gtatgcgccg
     3001 ataagcactg actcgtcgct gattgaaacc gactggccga agtgatcacc ggaactggcg
     3061 tccgacgcct ggagccgctg ttgctcgtcc cacacatccg cgctgcgggt gaagacatag
     3121 gctgatccgg cgaacccggc gctgttctta tccgtcggcg cgccgataat cgccgtgtcg
     3181 ccgctaaggg caaccgaggc tccgaagtcg tcattaaggg cggcgtctga cgcctggagt
     3241 tgttgccgct cgcgccacac tcccgcgctc cgcgtgaaga tgtaagccgt gccgctctcc
     3301 acaccgttga agtaggtcga ggctgcgccg atcagcatcg tctccccact gattgagatc
     3361 gccgagccga agtggctgga gaactgggca tttgacgcct gcaaacgctg ctgctcgctc
     3421 cacacgcccg cgctccgggt gaagacgtag accgagcctg cgcgctcgcc accgctgttc
     3481 ttatcctcgg agacgccgag aatcgccgtg tcaccgctga tagcaaccga ggtattgaac
     3541 aagccgtcaa gcggggcggc atccgacgcc tgcaaacgct gctgctcgct ccatacgccc
     3601 gcgctgcggg tgaaaatgta ggccgaaccc gcatcaactc ctccgctgtt gtcatccagt
     3661 ggcgcgccga taatagctgt atcgccgctg atagcaatcg agtagccgaa atcgtcgccg
     3721 gtggccgcgt cggacgcgtg caacctctgt tgctgcatga acgggtcaac cgtcaaagga
     3781 tactgcgcgc ccgcgtcgtt cgcctgaggg cgcacctcgc gcccttcgtc cgcgcgcatc
     3841 ttctcgatgg ctgcgcgcaa gtcggcaggc acgtcgcttt gtgcttgggg cagcgattgt
     3901 ctccgctccg ctgcgcgccg ctcttcgtgc gcaatcacgc ggcgcaacgg agtgctcgtc
     3961 gcgtagaaga agaagaggca ggcgaccgca cacacgaagg ccagtgcgct gcgagagcgg
     4021 atgaggtaat gcataagcga ctccttggtg tcccgcgact gatgcccgtc aggttgacgg
     4081 atgacggatt gacgttcggg tcggaggatt tagagaagag tttgagagac gccgatacgc
     4141 cgatagtccg gctcaaaaat cctcgtgagg gtaacgcgcg gaatctatca cacgcctcat
     4201 cgctttacaa tgcgcccggc aatccacact tgcgcgcgta tctattgtgt gcgcaggcgt
     4261 cgcgcgttct cttcgcctcg cggcgcggga gaaagttttc cgttctcagg cgcgtgcgct
     4321 cgcgccgaaa ctctctccgg gcggtcgctt tgtttgacgc cgcgtgttgc cgcttgttag
     4381 ccttccgccc aacgggaggt caagtaagcg aaacatgaaa ctcgtcgttt tgggatcggg
     4441 cacgtccgtc ccgcaccccg ggcgcgcttc ggcggcgcac tggctggaaa ccgcaggcgg
     4501 ctcgacgctc ctgctcgaca tcagcgcggc ctccgttcat cgcatggcgc aggagggctt
     4561 ggattgggtc aacctcgacg ccgtctggat cagccatttc catctcgatc atctcggcgg
     4621 actcttcccc ttcctcttcg gcacgaagca cgcgcccgcc acgcagacgc gccgcaagcc
     4681 gctccacatc tacgcgccac acggcttgaa gaaactcttc gcagccttcg atgcggcggg
     4741 cgactacaac ctgcttaagc aacctttccc cgtcgaaatc cgcgaggtcg cgccgcgcgc
     4801 ggagtttgag attctccccg gcctgcgcgc cgagactttc tccacgccgc acaccgacga
     4861 gagcctcgcc atcaaattaa ccgaccgcga cgaggcttcg ctcgccttca cttccgacac
     4921 gggctacacc gatgcgctcg ccgccttcgc gcggaacgtc tccctctttc tcatggagtg
     4981 ctccttcttt cgcgacaagc ccgtcgaact ccatctcgaa ctcgccgaag ccatgcgcct
     5041 cgccgaaagt tccggcgcgc ggcgcgtcat gctctcgcac ctctatcccg aatgggacaa
     5101 ctacaacctc gtcgccgaag cgaagagact ctggcgcggc gaaaccatcg aagcgcgcga
     5161 cggcttgcgt ctggaaattt aggagttgtg cgctgtcagc ccgcagcttt caatcagagc
     5221 gatccttcaa ccctgacaat taacaacctg acaactcacg gctcatagct tctttatgaa
     5281 atcacgcctc atcatcaatc ccgtctccgg caccgacagc gcgccggatc atttgcagac
     5341 aatcaacgaa cggctgcgcg cagcctttgg cgagatggac atcgtgatga ccgtgggcgc
     5401 aggcgacgcc tctgcggcag cggaagcggc ggcagccagt ggcgtctacg atcaactgtt
     5461 cgtggcgggc ggcgacggca ctttgaacga ggtcgtaaac ggcgtggcgc gcgtcgcggg
     5521 tgggttcgag cgcatcacgt tcggactcgt cccgctcggc acggggaacg atttcgcgag
     5581 cgcgctcggg ctgccggaag agatcgaagc ggccatcgag attctcctgc gcggtcgcac
     5641 cgtgatgtgt gatgtcggcg cactcggcga ccgtcgcttc gtcaacgtct cggcgggcgg
     5701 cttcatcgcg gaagtctcgg acgcggtcaa cccgcaactc aagaccgtcg cgggcaagct
     5761 cgcctatctt gtcggcggcg cgcaagtgct gttcgactat gagccggtcg gcgcgcaggt
     5821 gcgctcgacg ggagaagacg ggcgcgtgag cgagcgcaag ctcgacgtgc agatgtttgc
     5881 cgtctgcaac tcgcgcatgg tgggcggcgg gcggctcatc gcgccgcacg cttacgtgga
     5941 cgacgggctg ctcgacgtgt gcctcgtcga agcgatgccc gtgatcgaat tcatcggcct
     6001 cttaacgagc gtctccgccg gagagcatct gacggacgaa cgcgtcagtt attttcgcgc
     6061 acgctctctc gaactcagct tcgcgcgcac catcaaagtc aacaccgacg gcgaagtgct
     6121 ggagacagat cattgccgct acacgctcga accgcgcgcc gcacgcttcc tcgccggaga
     6181 cgcgcccttc gccgcagagc aatagcgtct ccttgcgcgt ctgctgcctc cgggtgaaag
     6241 cgaaaagtag cgattagctg tgagttgtga gttgtgagcg gttggcaaat tggttgagcc
     6301 gcctgtgact cgcgcacgaa gcgaaggcgg ctcgattgta tctgctttca gttggcagct
     6361 tctaaagcag agagggcagt gctgtccaaa ccactgccct ctccgctgcg tgcgctggcg
     6421 ggggaattgt cagcgcacgc gaacctgcac ctgattggct tccagagcgt tgccggttgc
     6481 gcgttgcagt tcggcgacgg ctttgttgag ttcggtctgg gcgcgcagtt cgttgccgcg
     6541 cgccgtggcg aacgcgttct ggcgttcgag tacgagaaag aaggttgact gtcccgcctc
     6601 gaacttccgt tgctcgctcg cgtattgctg ctcggtcgcg ctgcgctgtg cggctgccgc
     6661 gcgcaggcgc gactccgccg tgcgcgtcac ctgcaaggcg ttccgcacct cgacttgaat
     6721 caactgctca agctgttcgc gctgcacgcg gatgcggtcg cgctcgacga gcgtgcgccc
     6781 gagttgcgcc tccgccgtgc ggttgcgcag agggagattg agttgcacgc ccacgcgaaa
     6841 gttgttgaag cgattcgcgc cgaggttggc gattgactgg ccgtagccgc cgacgagcgt
     6901 atccgggatc gcgcctgccg cgacgggcgg gagcggttgg attctcgtgc cgtcgggcag
     6961 cggcggggcg agcgagatga gttgattgat gcgctcgcgc gtggcgtcgc tcgaagcgga
     7021 gaaagggttc acgctcgtcg cagggtcata cgcgcccgcc agacccaccg cgccgtaact
     7081 gccgacgagg tcaacctgcg gcttcgtcgc ctcgcgcgcg agcctttcgt cgagcgcgtt
     7141 gatctcgcgc actacgtccg actgttgcag ttcggggcgc gtggcgagcg cggagcgcat
     7201 cgcgtcttcg agcgcgactg agggcggctg cacgtcaacc atgtcggtcg gcacgagcgc
     7261 gaccgaccac acttccgatt gccgattctc cgcgatcaaa ttcttcagag cattctccgc
     7321 acgaccgact tcatcgagcg cgacatagac gttctgctcg aagtttgcca cctgcgcttc
     7381 cgccgccacg atgtcgatgg gcgcgagttg tccttcggcg acgaggcggc ggttgtgttc
     7441 aagctgcgtg cgggcatcgc gcgcggagtc gcgctgcacc tgcaagttgc gcagggtgaa
     7501 gaccaaatcc caataggcac gctgcacgct cgtgatcgtt tcgatggcgc gctggcgaaa
     7561 ctgcgcatcg gtgagcgaga ggttcttctt ggcgatctcg atctggcggc gcgtggcgtc
     7621 gaagcgaaac ccgcgcagga gcggttgcgc ataggtgaag gagaaggccg tcgggtattg
     7681 cgggttgagc gcgttgaaga ggctgttggt cgtctggcgg ctgctcgaaa aatcgaagcg
     7741 gtaattgccg ccgccgcgcg gcgtcagacc ttcaaaacgc atcgtgccgc cgaggccgga
     7801 ggaggtgacc gcgccaccct cgccgccgct caggaaactc gccacgggcg tcttgttgcg
     7861 ttcgtaaaaa gtggtggtgt tgaagcgcgg gtcgtatgcg ccgcgtgccg ccgtcaagtc
     7921 gaactcggcg gcgcgcacgt tcgtccgcgc cacttcgatg tccttgccgt tcttcaaggc
     7981 gagttcgatg gcctcgcgca gcgcgagcgg gcgttgctcc gccatgttga cgcccacgcg
     8041 ttcgagcgcg gggaggggcg cggtggcggg cgcgcgataa tccggcgcga tggcgggcac
     8101 ggggagagcc gtctccgcct gcgcgttgga aggcgtcggc tgcgtttgcg cctgcgcctc
     8161 ggcggtcgtc gcgtccgtcg tcgcattggt tgtcggcgtc ggcgttggtg tcggcgtgac
     8221 aggcgtttgc gtcgtcgttt gcggtgtcgc ttggggcgac ggctgcgcgg tctgctgctg
     8281 ctgcgccgga agcgtccgcg cctgtgcgcc cggctccgcg cccgtgcggg caggcttcga
     8341 cgctgcctcc tgtgaggcga ccatcaaagt tcccatcaga ggcacgtccg cgagttgcac
     8401 ttgcggcgac gcgtgctgcc gagcggcagc ggaagtcaca ggcgttgacg gctgcgtgtt
     8461 cgtctgttgc gccggggcgt cgcgcgccgc gagcaggagc gcgagcaaga gcgcgtgcgc
     8521 gcagaacttt ttagtcgaat aatgattcat gagtccgaac cgcgaattta agattttcct
     8581 gttcatcaaa acttattgaa gatcgccccg gcggctgtcg ccgtgcgtct acgcgcccac
     8641 gcaaacgcgc cgccaatgcc gctcccgacg cgcccgaaga ccttgctccg accgatatcg
     8701 tcaaagagcg aatagaagac cggcacggcg agcagcgtta acagcaggca gagcgattga
     8761 ccgccgacga cgagcacgcc gatggaacgg ttcgtgcccg aacccggacc gcttgagatg
     8821 acgagcggcg tcatgcccgc gacgagcgcg atggtcgtca tcaagatcgg acgcaggcgg
     8881 tcgcggttcg cctgaatgat cgcgtcgtgc cggtccatgc ccttcgcgcg cagttcgttc
     8941 gtgtggtcga tctgcaagat cgcgttcttc ttcacgacgc cgaagagcag gagcaggccg
     9001 aggccggaaa agatgttgac cgtctgcccc gtcagcagca gcgaaaggat gccgaacggg
     9061 atggcgagcg gcagcgtcag gaggatcgtc accggatgga tgaacgattc aaactgcgcg
     9121 gcgagcacca tgtacatgaa gatgaaggag agcgtgatgg cgagcaggaa gtaagcgccc
     9181 gcgcgcccca gctctttcga gcgaccgacg agtccggtgt tgtagcccga ttcgaaattc
     9241 atcgccgcga cggcgttgtc cagttccgcc agcaccgccg actgcgaacc gcccggcgcc
     9301 acgttggccg tcagagtcac ctgccgctgc cggttcaggc gatcaatcgc agacgggccg
     9361 ctcccctctt cgatgcgcac gaggttatcc agactgacga gtccgagctt cgccgaaggc
     9421 gcgaacaggc gcgacaactc ctctttgctc gcgcgcgctg cgccgatggc gcgcaggcgc
     9481 acgtcgtatt gatccgtacc ctgattgaag gtcgaaactt tttgtcccgc gaccatcgtg
     9541 ttcaaagact gcgcgatgtc gccgacgcgc acgccgaggt ctgccgcccg cgcgcggtcg
     9601 atgatgacgc gcaactccgg cttgcccgag atgagcgacg agtcaatgtc aaccacgttg
     9661 ggaatggtct tcatcttctc aagcaggtct tccgaatact tcgtcagcgt ttgcaggtcg
     9721 gggccgccga tcatgtactg aatgtcggcg ttgcgctggc cgccgcccga gatggcctgc
     9781 acctgcccga tgttggtgcg caactccggc gggtacttct tcaacagttc gcgcgccctg
     9841 accatcaagt ccgtctgcga caactcgcgt tcgctgaggt cggagagttt gacgtagatg
     9901 ttcgcgctgt tgacgagttg ctggcggtcg ccgccgatgg tgacgagcgt gtcggtcacg
     9961 cccggcaggg tgcgcacgtc cgccgcgatg cgttcgacta tcgtggaagt gttggcgagc
    10021 gtcgagcctt cgggcgcgcg catgttgatc tcgaactgcg attgatcgtc tacgggcaga
    10081 aagtttttgc cgacaaacat gaagagcggc acgatgctca caataaccag cacgcacagc
    10141 ccgacgattg cccagcgatg cgccatcgac cacttgagca tcgccgtgta agcgcgatcc
    10201 accgggcgat agaaacgccc gtccttcgag ccgtgcgact tcttctgttc gcgcccgcca
    10261 tcatccacat gcgtttcgga cggcagcact tccgcgtcca actgcgtcgc gctctccggc
    10321 tccgccttct ccggctcgcc cgccaccacg cgcgactcgt ttgcgagcag gctcgacgcc
    10381 tcgccctcgg aaggctcgct gcccactccc ggcgagacgg gctgcggctt cttgcgtttg
    10441 atcagacgcg cggcgagcat cggcgtcagc gtaaacgaaa cgatcaaaga gacggcgacg
    10501 gcgaacgagg ccgtcaggcc gaacgaagac atgaaacgtc cgacgatgcc gcccatgaaa
    10561 ccgacgggca aaaagaccgc cagcaggctg agcgtcgtcg ccatgacggc catgccgatc
    10621 tcgcgcgtgc cttcaatcgc cgcttgaaag ggcggcatgc ccttctcttc gatgaagcga
    10681 tagatgtttt ccagaacgac gatggcgtcg tcgatcacga tgccgaccat cagcgtgagc
    10741 gcgagcatcg tgatcatgtt cagggtgtag ccgagcgcgg ccatcaggcc gaaggtggcg
    10801 acgatggaag tcgggatggc gagtccggcg atcagggtcg aacggaaatt ccagagaaag
    10861 acgaagacga cgatggccgc gaggatgctg ccttcgatga ggtgcgtctg gatggactcg
    10921 accgccgcct tgatgaagat agacgaatcg ccgatcactt ccatgcgaat gttggacgga
    10981 agcgtgggct tgatctcttc gagccgcgcc ttgatctcgt cggccacagc cacggtgttc
    11041 gttcccgatt gcttggcgac gatgagcgtc acagccggtt cgccgttcag gcgcgcgctg
    11101 gtgcgcagtt cctccgaaga gtcttcggcg tagccgatgt cgctgagctt gaccggataa
    11161 tctccgcgcg tcgagatggc gatgtggttg aagtccgaag ggttcatgat gcgacccaga
    11221 gtgcgcacgg tgagttcgcg cgagccttcg tccacgcgcc cgcccggcac ttcaaggttc
    11281 tgcgcgcgca ccgccgccgc cacttccggc acggtcacgt tgaaggcgcg catcttgtcg
    11341 gggtcgagtt tgagatgaat ctcgcggcgg ctgccgccca cgatctccac gttgccgacg
    11401 ccgttgatgc tctcgatgcg ttccttgatc tgcttgtcgg cgatctcggt cacgtcgcgc
    11461 agagagcgcg gcgcggagac ggcgatgcgc aggacgggcg cggcgtccgt gtcgagtttc
    11521 tggatgacag gctcttccgc cgtctccggc aggtcggaag tgatgaggtt aactttgtcc
    11581 ctgatctcct gcgcggcgat gtccgcgttc ttgtcgagca ggaattggac gaagacctgc
    11641 gagacgcctt ccacggaggt cgagcgcagt tcgtcaatgc cgctgattgt gttgacggcg
    11701 gcctcgacct tatccgtgat ctccgtctcg atctcgcgcg gcgacgcgcc gggattaacg
    11761 accgagatgg tgatcgtagg gaagtctatc ttcgggaaga gatcgacgcc gagcgagaag
    11821 aacgaaaaga ttcccaccac cgtcagcgac aggatcaaca tcgtcgcaaa caccgggcga
    11881 cggacgcata tttcagctag tttctgcatg atgcctcaat agagcgatgc gctgcgaact
    11941 gttagccgga agccaaaagc taagagcttg cagctaacag ctaacggctg ttattgcctg
    12001 acggcgatgc cgtccttcaa catctcgacg ttactggtgg cgacgagttc gtccacgctg
    12061 attccggttt tgatttcgac gagttcgccc tcgacctgcc cgagttgcac gacgcgttcc
    12121 tgcacacgcc cgtctcgaat cacgaagacg cggctgacat cctgctccgt gcgcacggcg
    12181 gtagaaggaa cgagcaccgc cgcgtcgccc ttcggctgcg agatgcgcac cgtcgcaaac
    12241 tgccccggtt tcaacaagcc ctcaccgttc tccacttcgg cttcgaccgt cagcgtgcgc
    12301 gaggtggcga cgatgttcgg cgagatgcgc gcgatgcgcc cggcgaacga gcgatccgga
    12361 taggcactcg tcgtgatcga gacggtctgg ccgggctgga tggtcgagat ggcctgttcg
    12421 ggaatgtcta tgcgcatgcg cagagggttc gtgcgtacga tggtggcgac cttcgaagtg
    12481 ggcgacacgt actcgccgag gtctgccggg cgatccgcca cgtagccgct gatcggcgca
    12541 tagacggcca cgtccgagac ggccttgcgc gcctgcgcca cctgcgcctc ggctgcgttt
    12601 gccgccgcac gcgccgtggc aatgcccgcg taattctgcc gcgccgcctg caacgccgct
    12661 tcgtattgtt gctgcaactg gtcgcgctgc gacttctgcc ggtcgtaatc cgcgcgcgac
    12721 acgtcgcccg attcgatcag acgctcgaag cgtcgcaact gcttctcggc cagttcgagc
    12781 gcgacgcgcg ccgcgccgac ttcggccacg cggttcggat caaacgcctg accggggcgc
    12841 aggccgatgc gcgcttccgc ctgcctgacg gctgactgag cctgctgcgc ctgcgcctgc
    12901 aactgtgtga gacgcagacg cgcatccgca tcgtcgaggc ggacgagcag agaaccgcgc
    12961 tgcacgtagc tgcccagatc gacgccgacg gccacgacgc gcccggcgac cgtcggcgca
    13021 acgtccgtct gctggtctgc ggcgaggctg cccgtcgctt ccacgtagcg cgggagttcg
    13081 cgtgtgatgg cggcggcggt cgaaacctgc accacttcgg gcgtggcgtt ggcggcggcc
    13141 tgcttgttct tcgcgtccgc gctcgacctg ccgcacccgc tcgcaaacag gctcaaggcc
    13201 gcgagcagca aaatgaccgt ggggagtgtg aatttatttc gagagctcat gatgactgtt
    13261 tttctttcgg gacaaatcgc tttcgcgcaa aagctacggc gaggcaaact atctcttctt
    13321 tactatctct tctttgctat ctcttctttt tggcgcgggt cgggttggcc gcgctgccgt
    13381 gtttcgctcg ttcgccgctg ccgttcgcgt tactactact gctgccgttg ctattgcgcg
    13441 aggcgggcgg cgggttatga gtcgtggcgt gggcagcaat acctctgagc agtatctctg
    13501 tgaactcttc tgcggcgcgc tcgttcggaa tgctgaggat gcggcggcgc gtgtcccaga
    13561 gaatgttatt gagcgagtgg tgtatgacca tgccgagaaa acagcgcacg acgatgttcg
    13621 ggtcgatatt gcgcatcgcg ccctcgcgct gccgctcgcg aatgtatgaa ccgagaaagt
    13681 cgtacatgtg gcggacgttg cgatcccaga acatgtctgc gagttcgtgt ccttcgagcg
    13741 cggactgcgt caggaggcgc aggaattccg tgtcgtgttc atggtggcgc atcatggcga
    13801 gcgcgatgcc ggagaagacc gcgcggtcgt ctttgcgcgc aagcgcatcg gggacgatat
    13861 cgcaaggttg ctggaagttg gcggcgcagg ctttgtgatc gatgatcgcg ctgtagagct
    13921 cctgcttgtt ggcgaaatgg cgaaagacca tcgcctcgga gacgcccgcc gcgtgcgcga
    13981 tctctttcgt agtcgtgccg cgaaagccgc gcttggaaaa gaggctcatc gccacctgca
    14041 aaatctggcg acgacgatct tctcccgcca tccgcccgct gctgttcgcg cccgtcgctg
    14101 tcccggcggc gacagcttcg gtcgcgccct tcgtctctaa cacacggctc attcggttct
    14161 cgcctttcaa ctcccgcttc cgattaaaag taagtgctta cttacttacc aaagtacgag
    14221 ggacgtgtca agcgagttca cattaatttt cgcaccgcga tttttttgcg cgcccctgta
    14281 ttgttataga cttccgcgct ataatggcgc gtaccttttc acaacaaagt gaatgcgttg
    14341 cctcgctgca agaacctcgc gggtcttctt gtaaaggcgt ctttcggcgt tgccgtccgg
    14401 gcaggactcg tcggcacgtt gtttgctcgc ttgacaccgg aaatgcagaa ataggcgggc
    14461 gttagatatt cgcgcgcacg gtaatttaaa gagcaactat caacagttaa ggagataaag
    14521 gacatgtcac agaacttcgg aaacaacgat ccgctaaaca cgggcggcaa caccggcacg
    14581 accggcggaa caggtctggc aggcacgggc agaaccgatg aaggaacgac gcgcgacact
    14641 tcaacgggcg ggtcgggcat tggtggcggt tcgggtgtgg gcggcggact gagcaccacg
    14701 ggcggcgcga cgggcggcac gagcaccggc acggcgaccg cgcgcgaagg catcgcgggc
    14761 acggcgcagg agtacggtca gaagctcgcc gatgcggctt cgactgccaa agactatctg
    14821 ggcgacaaaa tctctgtggc gggcgacaag ttcaaagact tgcagaatgt agactaccgg
    14881 caggcagccg agcaggccaa aaactacgcg cgcgagaaac cgggacaggc gctcttgatc
    14941 tcggcggcgg cgggtttcct catcgggctt ctgctcaaga gcagcaaccg tcgttaattc
    15001 ggtttcaagg ttcaagtttc aggttacaaa ttaaaagcac atttcagcca caaacctgaa
    15061 acttgaaacc ttcaatttga aacttgagct taacgaggag ggtgaacgat ggctgaatta
    15121 gagaggcgtg ccgccggagg ctcggcggcg accgcgcctg ccaccactgc ggcgcagaac
    15181 gcgcaaggtc tggatatcga gggcttgccc gccatgctgg ggcggctcgg cgacaacgtg
    15241 atgacgctcg ttgacgccaa gctcgggctg gcgaaagtcg aactcaagga agaggc
//
LOCUS       DASWNS010000400        20851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_224969, whole genome shotgun sequence.
ACCESSION   DASWNS010000400 DASWNS010000000
VERSION     DASWNS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20851
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224969"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1329
                     /locus_tag="VGB76_14030"
     CDS             109..1329
                     /locus_tag="VGB76_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEY0100063.1"
                     /translation="MLARYMEASREIPTATSFRTMTVTAMDARRKQLKNAGEKVSFTH
                     LIAYAIARAATEEMPVMAHHFAEVDGKPHRVDDGAVNLGIAVDVEKKDGSRTLMVPVI
                     RDAGRRTFPEFLDAFGNLIARTGSTPNDYLYAGEQFDPNIGFYYLRARYMNPSNGRFQ
                     TMDEFEGVRHDPLTLHKYLYANANPVDGTDPSGHWTVGGLAATMAIGGIINSMIGMIF
                     SQHSPSSSEFWGEAAWNFGVGAVTAPVGGLATRLLAPLARATLGPLLQIVGRMGPIML
                     RGRGAVGQMLVRISRFFVNTNRSYPRVDSTGLGRLLQRAFPNIRWEHHHIYIQQSWSR
                     VGGPNQIYQNVDANEGLRRIGNGLWNMIPIPRELNAALGRSELGTQLFATAYYSILVY
                     GPAHTLAYFFDGED"
     gene            1394..1867
                     /locus_tag="VGB76_14035"
     CDS             1394..1867
                     /locus_tag="VGB76_14035"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEY0100064.1"
                     /translation="MTRALRRRRLERASRMTDEDIKFIERELELTLPESYKRALVPFG
                     IPLLAGNTKYELWDDAQELVKLNRELRAGSRFVPPWPPHLYAVGYPHGDEMIAVDTRD
                     PEGAVWWLDHGIVGHPASYQSHARFAEWVEEFYRDMRQDLEGDGFDPEAAPGSGE"
     gene            1986..2933
                     /locus_tag="VGB76_14040"
     CDS             1986..2933
                     /locus_tag="VGB76_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100065.1"
                     /translation="MRRVSIIALLVAALWGGANAQSKSDREHDGLRGAVRALKLEAAE
                     FTSKNGKSVEGARVLLRTVTYDARGNKTRQVVYNHDGSVAQTLVYASDPQGRSTGYEE
                     FTGTLSVPRKHVYVLDDKGNRIEYSLVQPDGSAGEKSLYKYDARGNVAEESLYDHKGG
                     LISRNVYAYDDRGRQVSQTRYNADGSLSATSALSYDAQDNLIERMRYESDILTYRVRY
                     VYDRRMRVIAQETVGSVVESDVPHSEAHAPGRVVYTYKGGEQPRELNIYAPDGSIRER
                     VVIEYDARGNWIRKTHLAESGKRGRWEARRVVRQTITYF"
     gene            3249..3902
                     /locus_tag="VGB76_14045"
     CDS             3249..3902
                     /locus_tag="VGB76_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100066.1"
                     /translation="MRLRSPKTLNARSAARAFLAATIAFAVVTGAVPLVPVFGAHGCS
                     MPCCKGADGMPGDCTGGSCPISHFGKAEAKVRSLKLAPADPSCHAGEETPPHDGAAGM
                     HHSKPGHASSPEQENDGIEHSAHRHVSPRDTSTEPAFTSAALSKPCPPGCGGILNAST
                     QLRRSRDEAALSDKLRPRPPTGELQTQDPAGVSKISFVLRRQYPPRAPPSISYSRLA"
     gene            4165..6978
                     /locus_tag="VGB76_14050"
     CDS             4165..6978
                     /locus_tag="VGB76_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0100067.1"
                     /translation="MTFILDHIRRGSPSILLCVLLLLGGLTSATAQQNVSSANLSGRV
                     EDAGGAAVSGANVSVNHVETNQRQTGVTDDEGRYRFLYLRVGTYQLTVEKSGFATLTR
                     QLTLTVGQALEVPLRLAVAGLAESVNVNETDVPLLETARTQVAETVVPQEIDSLPLNG
                     RNYLDLAALTPGVTRTNPVANQRFPETSSVPGTGLSITGQRFINNGFVVDGLSSNDDA
                     ADLAGTFYSQEVIREFEVITSGGIAEFGRASGGVVNIVTRSGTNKFGGRVYGFLRNQR
                     FDARNPLSPTKDPLTQGQYGASLGGRIKRDRTFFFSNFEQTRLNNAAVITITAGNVAA
                     VNARLDEIGYAGSRLSNGVAPTGFDTTNFLFRLDHRINEANQLTARYNLYDISSLNAR
                     NVGGLNAVSRGTALADRDQTIAASLVTTLSRRTINEARFQFTRSRLGAPVNEEVAPAV
                     NISGVANFGVATFSPTARDLDTFEAVNNVTTQRGAHSLKGGVNFLLNRVDITFPGALQ
                     GVYTFSSLANFQTGRYATFQQAFGAPGQFQSNPNVGLFVQDEWKPRSGLTINAGLRYD
                     AQFLPDPVRTDANNFAPRLGVAYAPGDHKTVFRASYGIFFDRLPLRATSNALQRDGSK
                     YRVAVLSFGQAGAPVFPNVLPEFPSNLLVSITTIDPDINNAYSQQASLQVERELSGAL
                     SLSVGYLHVRGAHIIFSRNVNVPRFAASAGVPNLGRPDPRFANVSRFESSGDSYYDGM
                     LVSFKRRFSRRAQMRVSYTLSKAIDNVGNAFFFTPQDNFNLRDERSLADNDQRHRLAV
                     SGSFEAPTAASDENMFRRSLSGFQLSYIFQYGSRLPFNVVTGADRNLDTNVNDRPTGI
                     GRNTGRGFDFASLDLRLSRRFHVGERANLETIVEGFNVLNRANLQLPNNTFGTGATPG
                     AGFGRPTAADNPRQVQFGLRLNF"
     gene            complement(7172..7393)
                     /locus_tag="VGB76_14055"
     CDS             complement(7172..7393)
                     /locus_tag="VGB76_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100068.1"
                     /translation="MRLWQDTNHNGISEPTELRTLPSLKVQAMSVDEKASKRVDEFGN
                     QFLYRAKLDDAKGAKVGRTAYDVFLVSIP"
     gene            7600..8997
                     /locus_tag="VGB76_14060"
     CDS             7600..8997
                     /locus_tag="VGB76_14060"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0100069.1"
                     /translation="MLSALNILLVFLLVFANGFFVASEFALVGVRRSRIATLAAAGDR
                     RARRLLGLVDNLNAYISATQLGITLASLALGWIGEPAVAHLLEAPLAGRVSETVLHTI
                     SFTIAFALITFLHIVLGELAPKTIALERAEKTALAIALPMQIFYKVFFYPIRLLDWAG
                     TRTVRLLGLHPSSDHASIYTEEELRSLIASSRQSGHIEEEEQQLINRVFDFSDAEVRE
                     AMIPRTNVEALPVTASFEEAKEAFRTLGYSRLPVYRERLDDIVGVVFRRDLEPYYDNP
                     APAVFDLEKIAHPPTFIPASARLGAVLKQMQGTRTHLAFVIDEHGGIEGIVTLEDLLE
                     EIVGEINDEYDEEVRSQITHEADGTYLVDGMLAVRDANRRFELNLPEDESYTTLAGFL
                     LARSGRLMQIGESVEHEGARFTVERVDRRRIRRIRYAPAEKGDEQTTAASYLAVLSLI
                     GMQFAESLQIIPVMF"
     gene            9342..9620
                     /locus_tag="VGB76_14065"
     CDS             9342..9620
                     /locus_tag="VGB76_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100070.1"
                     /translation="MLPSYAIDCHTPPGLVGKYYTKASNGFWHSFYFTTGGYPDPVRV
                     TRRNHNVTEQAYRKCHSEIVESIANHLRSPIMKFMHSRHSLSDASCGG"
     gene            10020..10997
                     /locus_tag="VGB76_14070"
     CDS             10020..10997
                     /locus_tag="VGB76_14070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0100071.1"
                     /translation="MKSAPSDCSRTLLRRPPNLHQHHADTNGTPSNLLSIRSSALLLA
                     SLILAALASSCSGGDQRAGEAQQPTLRLSMIPTTDPGKMLRESQPLIDYLQKETGAKI
                     EMTIPTNYAAVVEAMGSDQVDIAHFGGFTYVQASSRYGVRPLVQRERDRNFHSVFLTH
                     PSSAIDGLSDLKGRTFAFGDVNSTSGHLMPEYFMRQAGVDPEVIAKAAYTGGHDATAL
                     AVANKKVDAGALDELVFERMTKEGKLAPDSVRVFYTTPPFFDYIWAARKGLDPKVAEA
                     FANALLKLDAGDAQQKKVLDILNAAKYVKADDGDYEKLRQAARDAGLLK"
     gene            11007..11816
                     /locus_tag="VGB76_14075"
     CDS             11007..11816
                     /locus_tag="VGB76_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0100072.1"
                     /translation="MVYLLEQITKEYGTREGTTRALDGLSLSVKKGERLALIGRSGAG
                     KTTLFRLLNATLRPTRGVLRFDGRDVGRMQARELRAMRRRVGTVYQQHYLVPSLSVLD
                     NALCGRLGRWSLWQTARSIFRPARQDVEEAEHVLELVGLTDKLRERADALSGGQQQRL
                     AIARTLMQQPEVILADEPVASLDPALADSIVNLLHGVADKGNRTLVVALHNIDLALRY
                     FPRVVGLRGGAVAFDADAGGLNRAGLEEFYQDPETTPEGRGERAWRQAQVT"
     gene            11792..13744
                     /locus_tag="VGB76_14080"
     CDS             11792..13744
                     /locus_tag="VGB76_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0100073.1"
                     /translation="MATSSGYITRGESADFLTPRRVALLLAVAAALAFCWHAAEVRPT
                     TLLDRAAAVSVWSFLRGLFPPDLSVEFLRVVFAATVQTVAIAIAGTLLSVGIGMPLGI
                     LATATLWRRGVLLSGEHRGAFTTLLSLLSRLTRALLGFLRAIPDLMWGLLFVVAVGLG
                     SLPGTLALAVSYGGVLGRVYGDVFEDVDPRPLEALHATGATRAQVFLRAIWPQAAPAV
                     TAYTLYNFECAVRAAAILGFVGAGGIGYEINLSMRLFSYGQVLTLIMAFVVLLTTTDA
                     LSRFLRGRLNAEVTETSPVRYLMEETLGVGSRPFGRRASHVLARIRSRAVVPLFICAA
                     ACSLYAVGFLNGALLDAGVAGRLARFVGRMFPPDLSTEFLAGLAKPLFQTVGISVMGT
                     LIGITLGATLALPATSTLMFSRRDAAGRRSATGQAARWLAYHAARAIFTLLRAIPELV
                     WVLICILAVGLGPFAGTLAIGLHTGGVLGKLYAETLEEVPAKPVEALRAGGARPLQIF
                     LWAMWPQARALLLSYTVLRWEMNLRVSTVLGLVGGGGLGQAIYHNVQLGFYTRLSTLI
                     ALVCALVCATDWISDRLRLRTLAHGGAALELTPAGRPALREIEARTCEPLRDGDPLSL
                     QAGGQPRPVFSESNAGGAKNETRSQK"
     gene            13722..13934
                     /locus_tag="VGB76_14085"
     CDS             13722..13934
                     /locus_tag="VGB76_14085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY0100074.1"
                     /translation="MKHGARNKIVGTVTSVKKGDIMSLVKLNIAAPAEMSSVITTESA
                     EELELKEGDQVELIIKAVHVLPVKLE"
     gene            complement(14179..15597)
                     /locus_tag="VGB76_14090"
     CDS             complement(14179..15597)
                     /locus_tag="VGB76_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0100075.1"
                     /translation="MNLIKQALGCCLLLVVLQNSFVPSISAQTPNASPASSTLTAQQL
                     AVKVDEYMNAAAKAEGFSGSILVARDGSTVASKGYGLANIELNVPNTPQTVFRLASLT
                     KQFTALAIMMLQERGKLSTGDSICKYLDACPATWQPITIRQLLTHTSGIPNYTSFPDF
                     ARTAVLPATHESFIGRVKDRPLDFVAGEKFNYSNTGYYLLGVVVERAAGKSYEDFVQE
                     NIFTPLGMKNTGFDNPVRIIKNRAAGYEGQGERIHNASYIDMSNGYAAGFIVSTTEDL
                     LLWDKALYTERLVSRKSLDEMFTPFKDLSPTKNYAYAWWLEKQSDRQAISHSGHINGF
                     ATYIMRYPAERVTVIVLSNNRGAPSERVAKDLSAIAFGAPYKIPQERKVIAVASAVLE
                     KYAGQYQLRPNFIITMSLDNGKLLMQPNAPQKVEMFATSETEFFLKAGNAQFTFLKDA
                     QGTVTGLIFHLNGQETTAPKIK"
     gene            complement(16206..19670)
                     /locus_tag="VGB76_14095"
     CDS             complement(16206..19670)
                     /locus_tag="VGB76_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0100076.1"
                     /translation="MSFPKSLRPRASLAVLVLAACALLLSYSLPTTNASHTPNPTSVT
                     VAGSLQSELGCPGDWQPECGATHLNYDAGDDVWQGTFPVPAGNWEYKAPLNDNWGENY
                     GAGAQQNGANIPLSLGSGQNVKFYYDHKSHWITSNRNSVIAVAPGSFQSELGCSGDWD
                     PSCLRSWLQDPDGDGTYTFQTTALPAGNYESKVALNESWDENYGAGGEQNGANISFNV
                     PSNNAQVTFSYNAATHVLSIETANTPPPSGNVEWDGLRHDTFDSYYRSPFGAVPAGTD
                     VTLRFRTAHFDVEGVSVRVYTFDPATGATTGPVDHPMSFLENRTEGGTEYDIWTRALP
                     TPASPAILYYKFRVTDGADEDFYSDSYSDDHDNLNQGGAGAASDGEPFPAFQITAYDP
                     NFQTPAWLHNANVYQIFPDRFRNGDPTNDYCRPGSTAGCPTFYGDQKPLLREIWNAAI
                     GDPRQPGEFDGQYGTQFYGGDLKGIEQQLDYLQSVGIDTLYLNPIFKAVSNHRYDTDN
                     FLEVDPALGGDAALASLKAELERRGMRLILDGVFNHSSSDSIYFDLYQRYAQTDGGCE
                     NTSSPYRNWFNFFNSNAPCAYGDYEAWFGFGSLPVFRDDSAGVRDFFYRAPGANVTQY
                     WYERGASGWRFDVANEISHDWWKEYRPLAKGYKSDGPLVGEIWPDASQWLAGDQLDSV
                     MNYRFRKNVLGFARGQFNWSDNDNSGGNTIVALSPSQFDRALRSVREDYPAAATAAML
                     NLIDSHDTNRSLYVLTAGGDNGLAQAKERLKLSALFQFTYPGAPMVYYGDEAALNAPS
                     LANGVNGPEDDPYNRAPYPWADEAGDPNVYGPADQTVVNYYTTLAHMRKQHPALRNGS
                     FETLLTGDTSPSATDDNTYAFARVGGGETAIVALNNGAGGNTASLPVAAYFADGAQLR
                     DALSGATYAVGGGSVNLTLDARSGAVLFDASIAPVDTNAPVAAIAVNPPAGANGWHNS
                     TPVTVNLTATDGGSGVKELRFWINDGGVTVVEGDAASVQINAEGVNTISVRAIDNAGN
                     VSSVATQVVKLDLTPPVIGGASANPAVLSSPNHKMRDVTVSYNASDNFGNAACTLGVE
                     SNEAVSGTSDGDTAPDWEVLDAHRVRLRAERSGAGTGRVYTVNITCADAAGNTSNRAV
                     TVTVPRGR"
     gene            complement(19906..>20851)
                     /locus_tag="VGB76_14100"
     CDS             complement(19906..>20851)
                     /locus_tag="VGB76_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY0100077.1"
                     /translation="VKSPDARLNLNGADSLRRKGVIYMLAIGLNEYENANFNLKYAVA
                     DARSFASAFIRQQSQLGRYAGVKPVGLLDRNATKANIMRALAGLARQVQPEDAVVVYF
                     AGHGMAYQNQFYLLPHDLGYAGGRNQLDDAGLQLMLSRSVSDRELETAFEGVDAGSFL
                     LVIDACNSGQALEAEEGRRGPMNAKGLAQLAYEKGMYVLTAAQSFQAAQEVSKLGHGL
                     LTYVLIEEGLTQKAADAAPKDGRILLREWLDFTTRRVPEMQLDEIERSIERGGDLSFA
                     DEVRGLSVRKKPQQRVSQRPRVFYRRESEAAPLVVSRQ"
BASE COUNT         3921 a         6458 c         6433 g         4039 t
ORIGIN      
        1 gccgacgtcc tcgccgccaa ggccgccccg ctgcccgctc ccgctcccgc gccggccccg
       61 gccgccgaca ggcccgccgg cgccaccgag ctcaagggcg gcgccgcgat gctcgcgcgc
      121 tacatggagg cctcgcgcga gatccccacg gcgacgtcgt ttcgcacgat gaccgtcacg
      181 gcgatggacg cccggcgcaa gcagctgaag aacgccggcg agaaggtctc cttcacccac
      241 ctcatcgcct acgcgatcgc acgcgcggcg acggaggaga tgccggtgat ggcgcaccac
      301 ttcgccgagg tcgacggcaa gccgcaccgc gtcgacgacg gcgccgtgaa cctcggcatc
      361 gccgtcgacg tcgagaagaa ggacggcagc cgcacgctca tggtgccggt catccgcgac
      421 gccggccgca ggacgttccc cgagttcctc gacgcgttcg gcaacctcat cgcccgcacc
      481 ggctcgacgc cgaacgacta cctctacgca ggcgagcagt tcgacccgaa catcggcttc
      541 tactacctgc gcgcgaggta catgaacccg tccaacggaa gattccagac gatggacgag
      601 ttcgaaggcg tgcgccacga cccgctcacg ctgcacaagt acctgtacgc caacgccaac
      661 ccggttgacg gcacagaccc cagcggccac tggacggtcg ggggcttggc ggcgacgatg
      721 gcgattggcg gcatcatcaa ctcgatgatc ggcatgatct tcagccagca cagcccttcc
      781 tcttccgagt tctggggcga ggcggcgtgg aacttcggcg tcggcgcggt aactgcgccc
      841 gtcggcggac tcgccacccg gctgctcgcg ccgctcgcgc gcgccacgct gggtccactc
      901 ctccagattg tcggtcgaat gggaccgatc atgctcaggg ggagaggcgc ggtcggacag
      961 atgctggtga ggatcagccg cttcttcgtc aacacgaaca ggagctaccc gcgtgtggac
     1021 tccaccggcc tcggcaggct cctgcaaaga gccttcccga acatccgttg ggagcaccac
     1081 cacatctaca tccagcagtc ctggtcacgc gtcggaggcc cgaaccagat ttatcagaac
     1141 gtggacgcca acgaaggact caggcggatc gggaacgggt tgtggaatat gatccccatc
     1201 ccacgcgaac tgaacgctgc tctggggcgc agcgaactcg gcacacagtt gttcgccacc
     1261 gcctattact ccatcctcgt gtacggcccc gcgcacactc tggcctactt cttcgacggg
     1321 gaggactgaa gttcgctgcg tcggcctaac cgttagaacc ggagcgcacg agtcacgcgg
     1381 atgcaaacgc cgcgtgactc gtgcgctccg ccgcaggcgt ctggagcgcg cctctcgtat
     1441 gacggacgaa gacatcaagt tcatcgagcg tgaactcgaa ctcacgctgc cggaatccta
     1501 caaacgcgcc ctcgtgccct tcggtattcc gctgctcgcg ggcaacacta aatacgaact
     1561 gtgggacgat gcgcaggagt tggttaagct caaccgggaa ctgcgcgcgg gcagcaggtt
     1621 tgttccgccc tggccgccac acctgtatgc tgtcggctat ccccacggag acgaaatgat
     1681 cgccgtggac acgcgggacc ccgaaggtgc tgtgtggtgg ctcgaccacg gcatcgtcgg
     1741 gcatccggcg agctatcaat cacacgctcg ctttgccgaa tgggtcgaag agttctaccg
     1801 cgatatgcgg caagacctcg aaggagacgg cttcgacccg gaagcggctc ccggctccgg
     1861 cgagtagaca tccgtggcga gggtcgctcc ttcgccgtta atgacgcggg catcttgccc
     1921 tcgtcaatcc tgttaagcgt caatcttggc aggcgtaagt cttgaaattc gggagtgtgg
     1981 gagagatgag aagagtcagt atcatcgcat tgctcgtcgc cgcgttgtgg ggcggggcga
     2041 acgcgcagtc gaagagcgat agagaacacg acgggctgcg cggggcggtt cgcgcgttga
     2101 agttagaggc ggcggagttc acgtcgaaga acgggaagag cgtcgaaggt gcgcgcgtgc
     2161 ttttgcgcac ggtcacttac gacgcacgcg gcaacaagac ccgtcaggtt gtctacaacc
     2221 acgacggctc cgtcgcgcag acgctcgttt acgcctccga cccgcagggg cgaagcacgg
     2281 gctatgaaga gttcacgggc acgctgagcg tgccacgtaa gcatgtctac gtgctggacg
     2341 ataagggaaa ccggatcgaa tacagtctgg tgcagccgga cggctccgcg ggcgagaagt
     2401 ctctctacaa atacgatgcg agagggaacg tggccgagga gtctctctac gatcacaagg
     2461 gcggactcat cagccgaaac gtctacgcct acgacgacag gggcaggcaa gtctctcaaa
     2521 ccagatacaa cgccgacggc tccctctctg cgacaagcgc tctctcatac gatgcacagg
     2581 acaatctcat cgagcgtatg cggtatgaga gcgacattct tacgtatcgt gtgcgatacg
     2641 tctacgaccg caggatgagg gtgatcgcac aggagactgt cgggtcggtg gtcgagtctg
     2701 acgtgcctca ttcggaggcg cacgctcccg gtagagtggt ctatacctac aaaggcgggg
     2761 aacagccaag agagttgaac atctacgctc cggacggctc gatcagagaa agagttgtga
     2821 tcgaatacga cgcgcgcgga aattggatca ggaagactca tctggccgag tcagggaagc
     2881 gaggccgttg ggaagcgcga cgcgtcgtgc gccaaacgat cacatacttc taaaaagcta
     2941 cccgtgctgc acacggcggg tcgaagacat tcccgtgcga gttcgcgccc ctgctctgtt
     3001 gtcaggcggc gcacaccttc gccagcgcgc cagcatgccg gcgcgtcagc ccacttcccg
     3061 cgtatcgcct ccccatacac ctgccgcttc ttctcacgat gaataagcat cctccggcaa
     3121 taatccgacg agtcaccgca tcgcctgatc gtcggcctgc ggcaccagtc ttgccatcgt
     3181 ctttcttgca gtactacaat cggcgtgctt cctaaccgtc atccggcctg ctcaaacgaa
     3241 ggattaactt gcgtctgcga tcaccaaaaa cgctaaacgc gcgttcagcc gccagagctt
     3301 ttctggcggc gacaatagcg ttcgcggtcg tgacgggcgc agtgccgctc gtccccgtct
     3361 tcggcgcgca cggctgctcg atgccgtgct gcaaaggcgc ggacggcatg cccggcgatt
     3421 gcacgggcgg ctcgtgcccg atctcccatt tcggaaaagc agaagctaaa gtcaggtcgt
     3481 tgaaactcgc gcccgcagac ccctcgtgcc acgcgggcga ggaaacgccg ccgcatgacg
     3541 gcgcggcggg catgcaccat tccaagccgg ggcacgcctc ttcgcccgaa caagagaatg
     3601 acggaataga acactctgcc caccggcatg tctcaccacg cgacacctcg acggaacctg
     3661 cgtttacttc cgccgcgctc tcgaagccct gtcctcccgg ctgcggcgga attctaaacg
     3721 cctccaccca actgcgtcgc tcgcgtgacg aggctgcgct ctcggataag ctccggccac
     3781 gcccgcccac cggggaactg caaacacaag accccgccgg agtctctaaa atctccttcg
     3841 tgctgcgtcg ccagtacccg ccccgcgcgc ctccgtcgat ctcatacagc agactagctt
     3901 aatcatctag agggactccg aagccggacg cgcttcgtgg cgcagccatt ctaactgtcg
     3961 ccgcgtttcg cgtcgccgtc tgttcgcatg cagttcgtta tgcgcgacgg ctggggatgc
     4021 cttcagttag cactacttca gcacaccagc ttcgctagct ctggccgggt tcgacgcgag
     4081 gatagcctca ccctccccgg cttagagcgc ggcgtgcaat cgtgatgcgg cggtgtggta
     4141 cgaacatttt cggagcgaag acagatgact ttcatacttg atcatatcag gcggggaagc
     4201 ccgtcaatcc tgctgtgtgt gctcctgctg ctcggtggct taacgtctgc gacggctcaa
     4261 cagaacgtgt cttcggcgaa cctgagcggg cgcgtggagg acgcgggcgg cgcggcggtg
     4321 agcggggcga acgtctccgt caatcacgtc gaaaccaacc aacgacagac cggcgtgacc
     4381 gacgacgaag ggcgttatag atttctctac ctgcgagtcg ggacgtacca actcacggtc
     4441 gagaagagcg gcttcgcgac attaacaagg cagctaactt taactgtcgg gcaggcgctt
     4501 gaagtgcctt tgcggctggc tgtcgccgga ctcgccgaga gtgtcaatgt caacgagacg
     4561 gacgtgccgt tgctggagac ggcgcgcacg caggtggccg agacggtcgt gccgcaggag
     4621 attgacagcc tgcctctgaa cgggcgcaac tacctcgatc tggccgcgct gacgccgggc
     4681 gtcacgcgca ccaaccccgt cgccaaccaa cgcttcccgg aaacgtcgtc cgtgcccggc
     4741 acgggactct ccatcacagg gcaacgcttc atcaacaatg gcttcgtcgt tgatgggctg
     4801 tcgtcgaacg atgacgccgc cgatctggca gggacgtttt acagtcagga agtgatccgt
     4861 gagtttgagg tcattacctc cggcgggatc gcggagttcg ggcgcgcttc gggcggcgtc
     4921 gtcaacatcg tcacgcgttc gggcacgaac aagttcggcg ggcgcgtcta tggcttcctg
     4981 cgcaaccagc gtttcgacgc gcgcaacccg ctctccccga ccaaagaccc gctcacgcaa
     5041 gggcagtatg gcgcgtcgct cggcgggagg atcaagcgcg accgcacttt cttcttctcc
     5101 aatttcgagc agacgcgcct gaacaacgcc gccgtcatca ccatcactgc cggaaacgtc
     5161 gcggcggtca acgcacggct cgacgaaata ggttacgcgg ggtcgcgcct ctcgaacggg
     5221 gttgcgccca caggcttcga caccaccaac tttctcttcc gcctcgatca ccgcatcaat
     5281 gaggcgaacc aactgacggc gcgctataac ctgtacgaca tctcaagcct gaatgcgcgc
     5341 aacgtcggag gcttgaacgc cgtgagccgt ggcaccgcgc tcgctgaccg cgaccagaca
     5401 atcgccgcga gtctcgtcac caccctctcg cggcgcacca tcaacgaggc gcgcttccag
     5461 ttcacgcgca gccgactcgg cgcgccggtc aacgaagagg tcgcgcctgc cgtcaacatc
     5521 tccggtgtgg cgaacttcgg cgtcgccacc ttctcgccga cggcgcgcga ccttgacacc
     5581 ttcgaggccg tgaacaacgt caccacacag cgcggcgcac actcgctcaa aggcggagtt
     5641 aatttcctgc tcaaccgcgt ggacatcact tttcccggcg cgttgcaggg cgtctatacc
     5701 ttctcctcgc tcgctaattt tcaaacaggt cgctacgcca ctttccaaca ggcgttcggc
     5761 gcgccggggc agtttcagtc gaacccgaac gtcggcctct tcgtgcagga cgagtggaag
     5821 ccgcgctccg gtctgaccat caacgcgggg ctgcgctacg acgcgcaatt tctgcccgat
     5881 cccgtcagaa cggatgccaa caacttcgcg ccgagactcg gcgtggctta tgcgcccggc
     5941 gaccataaga cggtcttcag ggcgagctac ggtatcttct tcgaccgtct cccgctccgg
     6001 gctacgtcga acgccttgca gcgcgacggc tcgaagtatc gtgtggcggt gctctccttc
     6061 gggcaggcgg gcgcgcctgt cttcccgaac gtgctgccgg agtttccgtc gaacctgctc
     6121 gtcagcatca cgacgattga tcctgacatc aataacgcct acagccagca ggcgagcctg
     6181 caagtcgagc gcgagttgtc cggggcgttg tcgctttccg tcggttacct gcacgtacgg
     6241 ggcgcgcaca tcatcttctc ccgaaacgtc aacgtgccgc gcttcgcggc gtctgcggga
     6301 gtgccgaacc tggggcgacc cgacccgcgc ttcgccaacg tctcgcgctt cgaatcgtcg
     6361 ggcgattctt actacgacgg gatgctcgtc tccttcaagc ggcggttcag ccgtcgggcg
     6421 cagatgcgcg tctcctacac gctctcgaag gcgattgaca acgtgggcaa cgccttcttc
     6481 ttcacgccgc aggacaactt caacctgaga gacgagcgga gcctcgccga caacgaccag
     6541 cgacaccgcc tggccgtgag cggctcattc gaggctccga ccgccgcaag cgacgagaac
     6601 atgttccggc gttcgctctc cggcttccag ttgagctaca tcttccagta cggctcgcgc
     6661 ctgcccttca acgtcgtgac cggcgcagac cggaacctcg acacgaacgt caacgaccgc
     6721 cccacgggca taggccggaa caccggtcgc gggttcgact tcgcttcact cgacctgcgc
     6781 ctgtcacgcc ggttccacgt cggcgagcgc gcgaatctgg agacaatagt agagggcttc
     6841 aacgtgctca accgtgcgaa cctgcaactg cctaacaaca ccttcggcac gggtgcgacg
     6901 cccggtgcgg gcttcgggcg accgacggct gccgacaacc cgcgacaggt tcagttcggc
     6961 ctgcgtctca acttctgaga ggggtgatga tgaaagaacg aatgatgagc ggctgtctcg
     7021 cgccacgtct tcgccacatt tctctggaag ctggctgctg agtgggcaac taaagcggga
     7081 gttcaacggc tgccgaacac agtcttgaga agagagatcg ctcacaagtg atgtcacttg
     7141 tgaacgatct ctctttgagg ttcatcaatg actacgggat agagacaagg aacacgtcgt
     7201 aagccgtgcg cccgactttc gctcccttcg catcgtccaa tttcgcgcga tacagaaatt
     7261 gattcccaaa ctcgtctact ctcttcgatg ctttctcgtc aaccgacatc gcctgaacct
     7321 tgagcgaggg cagggtgcgt agctcggtcg gctcggagat accgttgtga ttcgtgtcct
     7381 gccagagacg caaactcgga aagattgcat cgctctgatc tatccagccg tctgcgttgc
     7441 ctccaggtct ggcattgctt attcaaaaag gagaatcctg agtagcggcg cgcaagatag
     7501 accgcttgaa agtgtggtgt ttagacacct ccggcagaac gaccggcttc gatgggcgcg
     7561 cgcgcttgtg agcagtcgtc gctgcattta tcattaagaa tgctttcagc cctgaacatc
     7621 ctgctcgttt ttctgctcgt ctttgctaac gggttctttg tcgcctcgga gtttgcgttg
     7681 gtcggcgtgc gccgttcacg gatcgcgacg ctcgccgctg cgggcgaccg ccgcgcacgg
     7741 cgtctcttag gtctggttga taatctcaac gcctacatct ccgctacaca actcggcatc
     7801 acgctcgcct cgctcgccct cggctggatc ggcgaacccg ccgtcgcgca cctgctcgaa
     7861 gcgcccctcg caggtcgcgt ctccgagacg gtgctccaca cgatctcctt caccatcgcg
     7921 tttgcgctga tcacgttcct ccatatcgtg ctcggcgaac tcgcgccgaa gaccatcgct
     7981 ctcgaacgcg cggagaaaac cgcgctcgcc atcgccctgc cgatgcagat tttctacaag
     8041 gtcttcttct acccgatccg cctgctcgat tgggctggca cccgcaccgt gcgcctgttg
     8101 gggctgcacc cttcgtccga ccacgcttcg atctatacgg aagaagaact ccgctcgctc
     8161 atcgcttcgt cgcgccagag cgggcacatc gaggaggaag agcagcaact catcaaccgc
     8221 gtcttcgact tctcggacgc cgaagtgcgt gaggcgatga tcccgcgcac gaacgtggaa
     8281 gccctccccg taaccgcatc cttcgaggaa gcgaaagagg ctttccgcac gctcggctat
     8341 tcgcgcctgc ccgtctatcg cgaacgactg gacgatatcg tcggcgtcgt cttccggcgt
     8401 gatctggagc cttactacga caaccccgcg cccgccgtct tcgacctgga aaagatcgcg
     8461 cacccgccga ccttcattcc cgcgtccgca cggctcggcg cagtgctcaa gcagatgcag
     8521 ggcacgcgca cgcacctcgc cttcgtgatc gacgagcacg gcggcatcga gggcatcgtg
     8581 acactggaag atttgctgga agagatcgtc ggtgagatca acgacgagta cgacgaggaa
     8641 gtgcgctcac agatcacgca cgaagccgac ggcacatatc tcgtcgacgg catgctcgcc
     8701 gtgcgggatg cgaaccgccg tttcgaactg aacctgccgg aggacgaaag ctacaccacg
     8761 ctcgcgggct tcctgctggc gcgcagcgga cgcctcatgc agattggcga gagcgtcgaa
     8821 catgaaggcg cgcggttcac ggtggagcgc gtagaccgcc gccgcatcag gcgcatccgc
     8881 tatgctccgg cagagaaagg cgatgagcag acaaccgcgg cgtcctattt agcagttctt
     8941 tcattgatcg gcatgcagtt cgccgagtcg ctgcaaatta ttccggtcat gttctaacgg
     9001 aagttgagcc tgacacgccg ggcggttctg ctaacagccg acccctccgc ccttcatatt
     9061 cgcaaccagt ctctgtaact atcagaaata ccgggatgga gatgcacgga cactgaaaca
     9121 gaagatcaca ggttttcacg aggacgaaca gggcgactgg gttgcggatt tggaatgcgg
     9181 gcaccagcag cacgttcgcc acaaaccgcc ctggatggtg cgtccgtggg ttgagtcaca
     9241 cgcgggtcga gagtcgcgca tcggcgtgga gttagattgt aagcggtgtg atgagatcaa
     9301 tgctgatttc gcaaccgaat tcaaataatt cattaacctg catgctccca agctatgcta
     9361 tagattgcca cacgccgccc ggcctcgtcg gcaaatacta cacgaaagca tccaacggct
     9421 tctggcactc cttctacttc acgacgggcg gctaccccga ccccgtccgc gtcacgcggc
     9481 gcaatcacaa cgtcacggag caggcgtacc gcaagtgcca ctcggagatc gtcgagtcaa
     9541 tcgcaaacca cctgcggagt ccgataatga aatttatgca ctctaggcac agcttatcgg
     9601 acgcaagctg tggagggtga gatgcctgct acatgagccg tcgcggattt cagtatcttg
     9661 acttgagcct ggtggcattg cacaatccgg ggcagagttt tccggactgc cagtgagtta
     9721 gccttgagcg cgtgatcgtg tgacgattgg cgtcggggcg tagaagctat catttgacag
     9781 ttacgaactg ttaggagagt tagagatgag agtcgtaagt cacgggcatt cgagggaacg
     9841 gcttagtccg acccttgtga accagaaaca atttaactga gcgggtttta cggcgtcccc
     9901 gtcggggttc gcaaaagacc cacaactcca caggctcgga agtagcttgc cgaacttcgt
     9961 ctaactgctc gacgaggcgc ttgctttctc gggtctgaag aaagcgagcc actcgtaaca
    10021 tgaaaagcgc cccgtccgat tgcagccgca ctcttctgcg gcgtcctcca aatcttcatc
    10081 aacatcacgc cgacacgaac ggcacaccgt cgaatcttct ctcgatacgc agttcggcat
    10141 tgctgctcgc gtctctaatt ctggccgcgc tggcgtcgag ttgttccggc ggcgaccagc
    10201 gcgcgggtga ggctcagcaa ccgacgctgc ggctgagtat gatcccaacg accgatcccg
    10261 gcaagatgct gcgcgagagc cagccgctca tagactactt gcagaaggag acgggcgcga
    10321 agatcgagat gaccatcccg acgaactacg cggcggtggt cgaggcgatg ggcagcgatc
    10381 aagtggacat cgcacacttc ggcggcttca cctacgtgca ggcgtcgagc cgctacggcg
    10441 tcaggccgct ggtccaacgc gaacgcgacc ggaactttca cagcgtcttc ctcacacacc
    10501 cttcatccgc catcgacgga ctgagcgatc tcaagggtcg caccttcgcc ttcggcgacg
    10561 tgaactctac ctccggccat ctcatgcccg aatactttat gcggcaggcg ggcgtagacc
    10621 cggaggtgat cgcgaaggct gcttacacag gcgggcacga cgccactgcc ctcgccgtcg
    10681 ccaacaagaa ggttgacgcg ggcgcgctcg acgaactcgt cttcgaacgg atgacgaagg
    10741 aggggaaact cgcgcccgat agcgtacgcg tcttttacac tacccctccc ttcttcgatt
    10801 acatctgggc ggcgcgcaaa gggctggacc cgaaagtggc ggaagccttc gccaatgccc
    10861 tgctcaaact cgacgccggg gacgcgcagc agaagaaggt gctcgacatc ctgaacgcgg
    10921 ccaaatacgt gaaggccgac gatggcgact acgaaaagct gcgacaggcg gcaagggacg
    10981 cagggctttt gaagtgactt ccaacgatgg tctacctgct cgaacagatc accaaagagt
    11041 acggcacgcg tgaaggcacg acgcgtgcgc tagacggcct ctcgctgtcg gtgaagaaag
    11101 gcgaacggct cgcgctcatc ggcaggtcgg gtgcgggcaa gacgacgctc tttcgtctcc
    11161 tcaacgcgac cctgcgcccg acgcgcggcg tcctgcgttt cgacgggcgc gacgtgggac
    11221 gtatgcaagc gcgtgagttg cgcgcgatgc ggcgacgcgt gggcacggtc taccagcagc
    11281 actatctggt gccatcgctg agcgtgctcg acaacgcgct ctgcgggcga ctcggtcgct
    11341 ggtctttgtg gcaaacggcg cgcagcatct tcagaccggc aaggcaggac gtggaggagg
    11401 ccgagcacgt gctcgaactc gtggggctga cggataagct gcgcgagcgt gccgacgcgc
    11461 tctccggcgg gcagcagcag cggctcgcca tcgcgcgcac gctgatgcag caacccgaag
    11521 tgattctggc cgacgagccg gtcgcctcgc tcgacccggc tctggccgat tcaatcgtga
    11581 acctgctgca cggcgttgcc gacaaaggta accgcacgct cgtcgtcgcc ctgcacaata
    11641 tcgatctggc gttgcgttac tttccgcgcg tcgtcggcct gcgcggcggc gcggtcgcct
    11701 tcgatgcgga cgccggagga ctgaaccgcg ccggactcga agagttttat caagacccgg
    11761 agacaacgcc ggaggggcgc ggcgagagag catggcgaca agctcaggtt acataacgcg
    11821 tggcgagagc gcggactttc tgacgcctcg acgcgtggct ctactgctcg ccgtggctgc
    11881 cgcgctcgcc ttctgctggc acgccgcaga ggttcgcccg acgacgcttc tcgaccgtgc
    11941 ggcggctgtc tccgtgtgga gtttcctgcg cggtcttttc ccgccggact tatccgtaga
    12001 gttcctgcgc gtcgtcttcg ctgcgacggt tcagaccgtc gccatcgcca tcgcgggcac
    12061 gctgctttcc gtcggcatcg ggatgccgct cggcatcctt gcgacggcga cactgtggcg
    12121 tcgcggcgtg ctgctctccg gcgagcatcg cggcgcgttc accacgcttc tctccttact
    12181 cagccggctg acgcgcgcac tcctcggctt tctgcgcgcc atcccagacc tgatgtgggg
    12241 gctgctcttc gtcgtcgcag taggcttggg atcgctgccg gggacgctgg cgttggcagt
    12301 cagttatggc ggcgtgctgg ggcgcgtcta cggagatgtc tttgaggacg ttgatccaag
    12361 accgctcgaa gccctgcacg ccacgggcgc gacccgcgca caggtgtttc tgcgcgccat
    12421 ctggccgcag gccgcccccg ccgtcaccgc ttacacgctc tacaacttcg agtgcgccgt
    12481 gcgcgccgcc gcgatcctcg ggttcgtcgg cgcgggcggc atcggctacg agatcaacct
    12541 ctcgatgcgc ctcttcagct acgggcaggt gctcacgctg attatggctt tcgtcgtgct
    12601 cctgacgacc accgacgccc tcagcaggtt cttacgcgga cgcctgaacg ccgaagtgac
    12661 cgagacatct cccgtgcgct acttgatgga ggagacgctc ggcgtcgggt cgcgtccttt
    12721 cgggcgtcgc gcctcgcacg tgctcgcgcg catcaggtcg cgcgccgtag tgcctctctt
    12781 catctgcgcc gccgcctgta gcctctacgc ggtcggcttc ctgaacggcg cgctgctcga
    12841 cgcgggcgtc gcaggccgcc tcgcacgttt cgtcggcagg atgttcccgc ccgacctttc
    12901 gacagagttc cttgccgggc tggcgaagcc gctcttccag accgtcggaa tctccgtgat
    12961 gggaacgctc atcggcatca cgctcggcgc gacgctcgcg ctgcccgcca cttcgacgct
    13021 gatgttctca aggcgtgatg cagccggacg ccgttcggcg acggggcagg ccgcgcgctg
    13081 gctcgcttac cacgcggcgc gggcgatctt cacgctgctg cgcgccatcc cggaactcgt
    13141 gtgggtgctc atctgcattc tggccgtggg actcggaccc ttcgccggga cgctcgccat
    13201 cgggctgcat acagggggcg tgctcggcaa gctctacgca gagacgctcg aagaggtgcc
    13261 tgcaaaacca gtggaggcgt tacgcgcagg gggcgcacgc ccgctccaga tttttctctg
    13321 ggcgatgtgg ccgcaggcgc gcgcgctgct cttgagttat acggtcttgc gttgggagat
    13381 gaatctgcgc gtctcgaccg tgctcgggct ggtcggcggc ggcgggcttg gtcaggccat
    13441 ctatcacaac gtccagttag gtttctacac gcgcctgagc acgctcatcg cgctcgtctg
    13501 cgcgctcgtc tgcgccaccg actggatcag cgaccggctg cgtttacgca cgctggcgca
    13561 cggcggcgcg gcgcttgaac tcacccctgc cggacgaccg gcgttgagag aaatagaggc
    13621 gaggacgtgc gagccgctgc gcgacggcga tcctctgtct ttacaggcgg gcggccagcc
    13681 gcgccccgtt ttcagtgaat caaacgcagg aggagcaaag aatgaaacac ggagccagaa
    13741 ataagatcgt ggggacggtt acgtccgtca agaagggcga tatcatgtcg ctcgtcaaac
    13801 tcaacatcgc cgccccggcg gagatgtctt cggtcatcac gacggagtcg gctgaagagc
    13861 ttgaactgaa agagggcgat caagtcgaac tgatcatcaa ggcggttcac gttctgcccg
    13921 tcaaactgga gtagaagaag ggctgctcaa gattagatat atgcgcaggg catcccgctc
    13981 atcttccagc ttttcgaggt gagaagtcga gctacgaatc agccacctcg ctcacacgtc
    14041 gcgtcgtgag cacacaaatg aagacgggca gccgtcaaag ctgcccgtct cttttttggc
    14101 cgttcgctgc ttttcatggc cgggatcgat gacgctgcgt gttcgaggcg gcgttaattt
    14161 gtaatgcggc tcttcggatt atttaatctt cggagccgta gtctcctgcc cgtttagatg
    14221 aaagataagt ccggtgacag ttccttgcgc gtctttgagg aacgtaaatt gtgcgttacc
    14281 cgctttgaga aaaaactctg tttccgaagt ggcgaacatt tcaaccttct gcggtgcgtt
    14341 cggttgcatc aacaactttc cgttgtccag actcatcgtg ataataaagt tcggacgcag
    14401 ttgatattgc ccggcatact tctcaagcac tgcgctggcg accgcgatga ctttgcgctc
    14461 ctgcggaatt ttatatggcg cgccgaaagc gatggccgat aagtctttgg ctactctctc
    14521 cgaaggtgcg ccccggttat tgctcaagac gatcaccgtg acgcgctccg cagggtagcg
    14581 catgatgtaa gtggcaaagc cgttgatgtg cccgctatga ctgatcgcct gacggtcgga
    14641 ctgcttttcg agccaccatg cataggcgta atttttggtg ggcgagaggt ctttgaaggg
    14701 cgtgaacatt tcgtcgagcg acttgcgcga aacgagtctc tccgtgtaga gagccttgtc
    14761 ccagagcaat aggtcttcgg ttgtcgaaac aatgaacccc gccgcgtatc cgttcgacat
    14821 atcgatatag gaggcgttgt ggattctctc gccttgccct tcgtacccgg cggcgcgatt
    14881 tttgatgatg cgtacagggt tgtcgaagcc tgtattcttc attcccaaag gcgtgaagat
    14941 gttctcctgc acaaagtctt cgtaagattt gcccgcagcg cgttcgacga cgacaccgag
    15001 caggtaataa cctgtgttgc tgtaattgaa cttttcgccc gccacgaaat ccaacggcct
    15061 atctttcacc cgcccgataa aactttcatg cgtcgcaggc aacacagccg tcctggcaaa
    15121 gtcagggaaa ctcgtgtagt tcggaatgcc ggaggtatgc gttaaaagct ggcgtatcgt
    15181 gatgggttgc cacgtcgccg ggcaagcatc cagatatttg cagatcgaat cgcccgtgct
    15241 cagtttgccg cgctcttgca gcatcatgat cgccagcgca gtgaactgct tggtcagcga
    15301 agccagacga aaaacggtct gcggcgtgtt cggcacgttc agttcgatgt tcgccagccc
    15361 gtagcctttg ctggcgacgg tcgagccgtc acgcgcgacg agaatcgagc cgctaaaacc
    15421 ttcggccttc gcggcggcgt tcatatactc gtcaacttta acggcgagct gctgcgcggt
    15481 cagcgtggag gacgcgggcg acgcgtttgg agtttgcgcg gagattgaag ggacgaagga
    15541 gttttgcagt acgacgagca gaaggcagca tccgagtgcc tgtttgatta aattcatcag
    15601 aaagctccgg ttgattaatt cgtaaatttg ctggttatga acaaagaggg attgtcgagt
    15661 aatcagggtt ggaacgcttt acgttcggga ataaatttat gtgaacggcg acaaagccgc
    15721 tccggtgtgg gaatttacct gcgtgcggca ctcgcgcacg tgcgccctgc gccgccttga
    15781 gaatgaccac ggaagagtat gccgtgcttc atttctatcc cacctgatac gcattactgg
    15841 tctgcgatca agcactgccc cgacaatata ctaaacgcgc aaaagcgagt tgtattacac
    15901 gtcttttagt aaaaacgcga tttttacgga atggcagttc aacctgtcgc ggggaacctc
    15961 tcagctatgc gcctcaccta tctcgcacgg ttcgccttaa tatcgaatag agacaactct
    16021 gttctacggc ttgtagacgc cgttgcctaa aggccgggtc aaagggttaa gcggaaatga
    16081 tgaccggggc aggagcagtt caacgccgcg acttcgaagg tcgcggcgtt gaactgcggt
    16141 cgtagaactt aaacgctggc aagtaatttt tgcgagccaa ccgccagcgc gtgcctctac
    16201 tgacgctaac gcccgcgcgg gacggtgacc gttacggcgc ggttcgacgt gttgccagcg
    16261 gcgtctgcgc aagtgatgtt gacggtgtag acgcgccccg tgcccgcgcc agatcgctca
    16321 gccctgagcc ttacacggtg agcgtccaag acctcccagt cgggcgcggt gtcgccgtcg
    16381 ctcgtgccgc tgaccgcctc gttgctctcg acgccgagtg tgcaggccgc gttgccgaag
    16441 ttgtccgagg cattgtagct aacagtcacg tcccgcatct tatgattagg tgacgacagg
    16501 actgcggggt ttgcggacgc gccgccgatg acaggcggcg tgaggtctag cttcactacc
    16561 tgcgtggcga cgctgctgac gtttccggcg ttgtctatgg cgcgcacgct gatcgtattg
    16621 acgccttcgg cattgatctg cacgctggcc gcgtcgcctt cgacaaccgt gacgccgccg
    16681 tcgttaatcc agaagcgcaa ctccttaacg ccgctgccgc cgtcggtcgc cgtgagattg
    16741 actgtcacgg gagtcgagtt gtgccagccg ttcgcaccgg cgggcgggtt gacggcgatg
    16801 gcggcgacgg gcgcgtttgt gtctacgggt gcgattgaag catcgaagag caccgcgccg
    16861 ctgcgtgcgt cgagcgtgag gttcacgctg ccaccgccca cggcgtaggt cgcgccgctc
    16921 aaggcgtcgc gaagctgcgc gccgtctgcg aaataggctg cgacggggag gctggccgtg
    16981 ttgccgcccg cgccgttgtt gagcgcgacg atggctgtct cgccgccgcc cacgcgtgcg
    17041 aaggcgtagg tgttgtcgtc ggtcgccgag gggctggtgt cacccgtgag cagcgtctcg
    17101 aaggagccgt tgcggagcgc agggtgctgc ttacgcatgt gcgcgagcgt cgtgtaatag
    17161 ttaacgaccg tctgatctgc ggggccgtag acgttcgggt cgcccgcttc gtcagcccag
    17221 gggtaaggcg cgcgattgta ggggtcgtcc tccggaccat tgacgccatt ggcgagcgaa
    17281 ggcgcgttga gcgcggcctc gtcgccgtag tagaccatcg gcgcgccggg atacgtgaac
    17341 tggaagagcg cggagagctt gagacgttct ttcgcctgcg ccagcccgtt gtcgccgcct
    17401 gccgtcagca cgtagagcga gcggttggtg tcgtgcgagt cgatcaggtt gagcatggct
    17461 gcggtcgcgg cggcggggta gtcctcgcgc acggagcgga gcgcgcggtc gaactggctc
    17521 ggactcagtg cgacgatggt gttcccgccg ctgttgtcgt tgtcgctcca gttaaactgg
    17581 ccgcgcgcga agccgaggac gttcttgcgg aaacgatagt tcatcacgga atcgagttga
    17641 tcgcctgcga gccactggct ggcgtccggc cagatttccc ctacgagcgg cccgtcggat
    17701 ttgtagcctt tggctaacgg acgatactcc ttccaccagt cgtgcgaaat ttcattcgcc
    17761 acgtcgaaac gccagcccga cgccccgcgc tcgtaccagt actgcgtgac gttcgcgccg
    17821 ggcgcgcggt agaagaagtc gcgcacgccc gccgagtcgt cacggaagac gggcaggctg
    17881 ccgaagccga accacgcttc gtaatcaccg taggcgcagg gggcgttcga gttgaagaag
    17941 ttgaaccagt tgcggtaggg cgaagaggtg ttctcgcacc cgccgtcggt ctgtgcatag
    18001 cgttggtaga ggtcgaagta gatgctgtcc gaagatgagt ggttgaaaac gccgtcgagg
    18061 atgaggcgca tgccgcgccg ctccaactcg gccttgagcg aggccagcgc ggcgtcgccg
    18121 ccgagcgcgg ggtcaacttc gaggaagttg tcggtgtcgt agcgatggtt ggagacggct
    18181 ttgaaaatgg gattgaggta gagcgtgtcg atgccgacgc tctgcaagta gtcgagttgc
    18241 tgctcgatgc ctttcaggtc gccgccatag aactgcgtgc cgtactggcc gtcgaactcg
    18301 ccgggctggc gcgggtcgcc gatggcggcg ttccagattt cgcgcaagag cggcttctgg
    18361 tcgccgtaga aggtggggca tccggcggtc gaaccggggc ggcagtagtc gttcgtcggg
    18421 tcgccgttgc ggaagcggtc ggggaatatc tggtagacgt tggcgttgtg gagccaggcg
    18481 ggcgtctgga agttcgggtc gtaggcagtg atttggaagg ccgggaacgg ctcgccgtcg
    18541 gaggccgcgc ccgcgccgcc ctgattgagg ttgtcgtgat cgtcgctgta ggagtcgctg
    18601 tagaaatcct cgtccgctcc gtccgtgacg cggaacttgt aatagagaat cgcgggcgac
    18661 gcgggcgtgg gaagtgcgcg cgtccagatg tcgtattcgg tgccgccttc ggtgcggttc
    18721 tcaaggaagg acatcggatg atcgacgggg ccggtggtcg cgccggtcgc cgggtcgaag
    18781 gtgtagacgc gtacggaaac gccttcgaca tcgaagtgcg cggtgcggaa acgcagcgtc
    18841 acatcggttc cggcgggcac cgcgccgaag gggctgcggt agtaactgtc gaacgtgtca
    18901 tggcgcagcc cgtcccactc gacgttgccg gaagggggcg gcgtgttggc cgtctcaatc
    18961 gacaggacgt gcgttgcggc gttgtagctg aaagtgacct gtgcgttgtt agacggcacg
    19021 ttgaaagaga tgttcgcgcc gttctgctcc ccgcccgcgc cgtagttttc gtcccaactt
    19081 tcgttgaggg ctaccttgct ctcgtaattt ccggcgggca gcgcggtggt ttgaaaggtg
    19141 taggtgccgt cgccgtcggg gtcttgcagc catgagcgga gacaggacgg gtcccagtcg
    19201 ccggagcaac cgagttccga ctggaaactg ccgggcgcga cggcgatgac ggaattgcgg
    19261 ttgctcgtaa tccagtgcga tttatggtcg taataaaatt tgacgttctg gccggagccg
    19321 agcgagagcg ggatgttcgc gccgttctgc tgcgcgcccg cgccgtagtt ctcgccccag
    19381 ttgtcgttga gcggagcctt gtactcccag ttaccggcgg gcacgggaaa cgtgccctgc
    19441 cacacgtcgt cgcccgcgtc gtagttcaaa tgcgtcgcgc cgcattcggg ttgccagtcg
    19501 ccggggcagc cgagttcgct ctggaggctt ccggcgacgg tgacgctcgt ggggttgggt
    19561 gtgtgcgacg cgttggtcgt gggaagactg taggagagca gcagcgcgca agctgccaga
    19621 acgagaaccg ccagcgacgc acgcgggcgt agagatttgg ggaacgacat gacgaactcc
    19681 ttgggggaga cccagaccgc ccgacaagcg accggaatcc cgggctgaga tactttttgt
    19741 aaatggcttg gcgaggctgt gaagaagatc gcgggaaaac gcagactttg gcagccagcc
    19801 ggactttgtt cgactccgca acgtacttta atcggcgcgg gaaatcaagg tttgagccgt
    19861 ggaatctgca aaaatatccg tgccgcccca caccagatat ttttatcact ggcgcgagac
    19921 gacgagcgga gccgcttcac tctcgcgccg gtaaaagacg cgtggccgct ggctcacgcg
    19981 ctgctgcggc ttcttccgta cgctcaggcc gcgcacttca tccgcgaagg agaggtcgcc
    20041 cccgcgctcg atggaacgct cgatctcgtc cagttgcatc tccggcacgc gccgcgtcgt
    20101 aaagtccagc cactcgcgca ggaggatgcg cccgtcttta ggcgcggcgt ccgcagcctt
    20161 ctgcgtcaag ccttcctcga tcaacacgta ggtcagcaag ccgtgcccca gtttggagac
    20221 ttcctgcgcc gcctgaaaac tctgcgccgc cgtgagcacg tacatcccct tctcataggc
    20281 caactgcgcc aagcccttcg cattcatagg gccacgccgc ccttcctccg cctccaacgc
    20341 ctgcccggaa ttgcaggcgt ctatcacgag cagaaaggag cctgcgtcaa ctccctcgaa
    20401 ggccgtctcc aactcgcggt cggagacgct gcgcgacagc atcaattgca gcccggcgtc
    20461 gtcgagttgg ttgcgcccgc ccgcgtaacc cagatcatgc ggcaacaggt agaactgatt
    20521 ttgataggcc atgccatgcc ccgcgaagta gacgacgacg gcatcttccg gctgcacctg
    20581 cctggcgagt cctgcgagcg cgcgcatgat gttcgccttc gtcgcgttgc gatcaaggag
    20641 gccgacgggt ttgacgcccg cgtaccgacc gagttgcgac tgctgcctga tgaaggccga
    20701 ggcgaaactg cgcgcgtccg cgaccgcata tttgagattg aagtttgcgt tctcgtattc
    20761 gttgaggccg atggcgagca tgtagatgac gcctttgcgt ctgagcgaat cggctccgtt
    20821 gaggttgagt cgcgcgtcgg gacttttgac g
//
LOCUS       DASWNS010000401         3946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_325157, whole genome shotgun sequence.
ACCESSION   DASWNS010000401 DASWNS010000000
VERSION     DASWNS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325157"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VGB76_14105"
     CDS             complement(<1..1112)
                     /locus_tag="VGB76_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY0100078.1"
                     /translation="MSTNGNGDYNQGDAAKNIAADTENIGGNVSGSGSSQTYPGPVVA
                     GEVAPPPAQLKKADFVSGQEVRWCPGCGDYSILNNVQKVMPELGIPREKIVFVSGIGC
                     SSRFPYYMNTYGFHSIHGRAPAVATGIKAANPELSVWVITGDGDALSIGGNHFMHAIR
                     RNLDLNYILFNNRIYGLTKGQYSPTSELGKRTKSTPMGVIDYPLNPLSVAIASEATFV
                     ARTLDLDVKHLGGIVQAGARHKGVSFIEVYQNCNIFNDGAFEYFTERSVRSDHMIYLE
                     HGQPMIFGKERNKGIRMNGAHPEVVTIGENGITVDDLLVHDIHLKDPSVAFMLARMEY
                     PDFPQPVGIFRAVERDTYEDMMGAQIEAAIAKQGPG"
     gene            complement(1109..1390)
                     /locus_tag="VGB76_14110"
     CDS             complement(1109..1390)
                     /locus_tag="VGB76_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100079.1"
                     /translation="MSKIRIEVIEGFDPNEWDIHREGRQWSDEEFDKRIYQAPEKIEF
                     VAGIFTNERERLTVLAMLLENLGIDKAVLLGNPADWKAAVAELTSKDKL"
     gene            complement(1397..1921)
                     /locus_tag="VGB76_14115"
     CDS             complement(1397..1921)
                     /locus_tag="VGB76_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100080.1"
                     /translation="MGKQMTGRELKVHRQKRHRVAGHYLALQAWMRRLDCIVLVREEL
                     EVFLDRERIRKEHVEHFATDVAPWFPHTKVFYLETKARPVNSLFLSRFPFPKSSSGSL
                     ETKARIAMINRTDGAPKTGRFSERKDANELPSHEKMVAYLNALAGGSKVSPLKSEIKA
                     ARLSVMQPVVFEKL"
     gene            complement(1936..3810)
                     /locus_tag="VGB76_14120"
     CDS             complement(1936..3810)
                     /locus_tag="VGB76_14120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY0100081.1"
                     /translation="MSTVIDPPSDAAPTVEKHFEEVETVTIRFAGDSGDGMQLTGTQF
                     TNTSAVLGNDISTLPDFPAEIRAPAGSLPGVSGFQLNFSSQDIRTPGDQPNVLVAMNP
                     AALKVNLFDLEEGGTIIVNTDEFNKENLKKAAYAVSPLEDETLKGYRVHRLPITTLNL
                     RALQEAQVKLTRKEMDRCKNFFALGVLYWLYDRPMQPTLEWIEAKFKKLPEVARANVV
                     ALRTGYNFADTTEVFTTHYSVKKAAIAPGKYRKITGNEATAIGFVAASELCGRPLFYG
                     SYPITPASDILHELARHKNFGVKTFQAEDEIAAVCAAIGASFTGHIGLTGTSGPGVAL
                     KQEAIGLAVMTELPLVIINVQRGGPSTGLPTKTEQADLFQAVWGRNGECPAVVVAPST
                     PADCFHMAVESVRLALKYMTPVFYLSDGYLANGAEPWALPAIADLPKIEVKFADDPAG
                     FLPYARDEQTLSRPFAIPGTPGLEHRVGGIEKEHLTGNVNYDPENHQFMVKLRQEKVD
                     RVAEDIPDLEIFGERTGKVLVLGWGSTYGSITSAVERLQREGRSVSSAHLRYLNPFPK
                     NLGEVLRSFERVIIPEMNLGQLATMIRAKYLVDAVTFSKVKGRPFQIREIVEKVEEYL
                     "
BASE COUNT          771 a         1097 c         1189 g          889 t
ORIGIN      
        1 ctgccgggtc cctgcttggc gatggcggct tcgatctgcg cgcccatcat gtcttcgtag
       61 gtgtcgcgct cgacggcgcg gaagatgccg acgggttgcg ggaagtcagg gtattccatg
      121 cgcgcgagca tgaaggcgac cgaagggtct ttgagatgta tgtcgtggac gagcaggtcg
      181 tccactgtga tgccgttctc gccgatggtc acgacctccg ggtgcgcgcc gttcatgcga
      241 atccccttat tgcgctcctt gccgaagatc atcggctggc cgtgttcgag atagatcatg
      301 tggtcggagc gcacgcttcg ttcggtgaag tattcaaacg cgccgtcgtt gaagatgttg
      361 cagttctgat agacctctat gaaggagacg cccttgtggc gcgcacccgc ctgcacgatg
      421 ccgccgagat gtttgacatc gagatcgagc gtgcgcgcga cgaacgtggc ctcgctggca
      481 atcgctaccg acagcgggtt gagcgggtag tcgataacgc ccatcggcgt tgacttcgtg
      541 cgcttgccga gttcggaggt ggggctgtac tgccctttcg tcaaaccgta gatgcggttg
      601 ttgaagagta tgtagttgag gtcgaggttg cggcggatgg cgtgcatgaa atggttgccg
      661 ccgatggaga gcgcgtcgcc gtcgcccgtg atgacccaga ccgacaactc cggattcgcg
      721 gccttgatgc cggtggcgac ggcgggcgcg cgcccgtgaa tcgagtggaa gccgtaggtg
      781 ttcatgtagt aggggaagcg cgaggagcag ccgatgccgc tgacgaagac gatcttctcg
      841 cgcgggatgc cgagttcggg catcaccttc tgcacgttgt tcaagatcga atagtcgccg
      901 cagcccggac accagcgcac ttcctgtccc gagacgaaat ccgccttctt caactgcgcc
      961 ggaggcggcg cgacctcgcc cgccacgacc ggacccgggt aggtttgcga ggaaccgctg
     1021 cccgagacat tcccgccgat gttctccgtg tcggcagcga tgtttttcgc cgcgtcgccc
     1081 tggttgtagt ctccgtttcc attagtgctc ataatttatc cttcgacgtt agttctgcaa
     1141 cagccgcttt ccagtccgct ggatttccca gaaggacggc tttatctatc ccaagattct
     1201 ccaacaacat cgccaacacc gttagccgct ctcgctcgtt cgtaaagatg cctgcgacaa
     1261 actcgatctt ttcgggagct tgataaatcc gcttatcgaa ctcctcgtca ctccactggc
     1321 gaccttcgcg gtgaatgtcc cattcatttg ggtcgaagcc ttcgatgacc tcaattctaa
     1381 tcttactcat catgtattaa agcttctcga aaacgacagg ctgcatcaca cttagccttg
     1441 ccgcttttat ttcacttttt agcggtgaca cttttgaacc accagcaagc gcattgagat
     1501 aagcaaccat tttttcatgt gacgggagtt cattggcatc cttacgttca gaaaatcgtc
     1561 cagtctttgg agcaccgtct gttcggttaa tcatggctat acgcgcctta gtttctaaag
     1621 aaccacttga agacttcgga aaaggaaatc ttgaaagaaa caaactatta acaggccgcg
     1681 cctttgtttc aaggtaaaac accttcgtat gaggaaacca aggggcaaca tctgtcgcga
     1741 agtgttcaac gtgctctttc cgaatgcgtt cgcgatctaa aaagacctcc aactcttcac
     1801 gaactagcac gatacaatca agacggcgca tccaagcctg aagagctaaa taatgtccgg
     1861 caacacgatg tcttttttgc cggtgtacct ttaattctcg gccagtcatt tgtttgccca
     1921 ctcaatattt aatacttaca aatactcttc caccttctcc acgatctcgc gtatctggaa
     1981 gggacgccct ttgactttgg agaacgtgac ggcgtcaacc agatacttcg cgcggatcat
     2041 ggtggcgagt tggcctagat tcatctccgg gatgatgacg cgctcgaacg aacggagcac
     2101 ctcaccgagg tttttcggga agggattcaa atagcgcagg tgcgcgctcg acacggagcg
     2161 gccttcgcgc tgcaagcgtt cgaccgccga agtgatcgag ccgtaagtcg aaccccagcc
     2221 gagcacgagc accttgcccg tccgttcgcc gaagatttcg aggtcgggaa tgtcctcagc
     2281 cacgcggtca accttctcct gcctgagttt caccatgaac tggtggttct ccgggtcgta
     2341 gttgacgttg ccggtgagat gttccttctc gatgccgccc acgcggtgtt cgaggccggg
     2401 cgtgccggga atggcgaagg ggcgcgaaag cgtctgctcg tcgcgcgcgt aggggagaaa
     2461 gcctgcgggg tcgtctgcaa acttaacttc gatcttcggc aggtctgcaa tcgcgggcag
     2521 tgcccaaggc tccgcgccgt tggcgagata accgtcggag aggtagaaca cgggcgtcat
     2581 gtacttgagg gcgaggcgca cggattcgac cgccatgtgg aaacagtctg cgggcgtcga
     2641 aggcgcgacg accaccgccg gacattcgcc gtttcgtccc cagacggctt gaaagagatc
     2701 ggcctgctcg gtcttcgtcg gcaatccggt cgaaggaccg ccgcgctgca cgttgatgat
     2761 gacgagcggg agttcggtca tcacggcgag gccgatggcc tcctgtttga gcgcgacgcc
     2821 gggaccggac gtgcccgtca agccgatgtg ccccgtgaac gatgcgccga tggcggcgca
     2881 gaccgccgcg atctcgtctt cggcctgaaa cgttttgacg ccgaaatttt tatggcgcgc
     2941 cagttcgtgc aggatgtcgg aggcgggcgt gatcgggtaa gagccgtaga agagcgggcg
     3001 accgcaaagt tcggaggcgg cgacgaagcc gatggcggtc gcttcgttac ccgtaatctt
     3061 gcgatacttg ccgggcgcaa tcgcggcctt cttgacgctg tagtgggtgg tgaaaacttc
     3121 ggtcgtgtcg gcgaagttgt agcccgtgcg cagggcgacg acgttggcgc gcgccacttc
     3181 gggcagcttc ttgaacttcg cttcgatcca ttcgagcgtc ggctgcatcg ggcggtcgta
     3241 gagccagtag agcacgccga gcgcgaagaa gtttttgcag cgatccatct ccttgcgcgt
     3301 cagtttcacc tgcgcctctt gcagcgcgcg aaggttcaga gtggtgatcg gcaggcggtg
     3361 gacgcgatag cctttgagcg tctcgtcttc gagcgggctg acggcgtagg ccgccttctt
     3421 caagttctct ttgttgaact cgtccgtgtt gacgatgatc gtgccgccct cttccagatc
     3481 gaacaggttg accttaagcg cagccgggtt catcgcgacg agcacgttcg gctggtcgcc
     3541 gggcgtgcgt atgtcttgag aggagaagtt caactggaag ccggagacgc cgggcaggct
     3601 tccggcggga gcgcgaatct ccgcggggaa gtcgggcagc gtcgagatgt cgttgccgag
     3661 gacggcggag gtgttcgtaa actgcgtgcc ggttaactgc atgccgtcgc cggagtctcc
     3721 ggcgaagcgg atggtcacgg tttcgacttc ctcgaaatgt ttttcgacag tcggtgctgc
     3781 gtcggatggc ggatcaatga cagtgctcat aatgtcttaa tgaatcctta ctgttttggg
     3841 aagataacga agcgccaatt caggtaggaa cacttcggtt catctggctc aagcgcattg
     3901 taacatagcc acacgcgccc gactcaagcg acgcggaaat cgttcg
//
LOCUS       DASWNS010000402         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_300698, whole genome shotgun sequence.
ACCESSION   DASWNS010000402 DASWNS010000000
VERSION     DASWNS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300698"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..651)
                     /locus_tag="VGB76_14125"
     CDS             complement(73..651)
                     /locus_tag="VGB76_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY0100082.1"
                     /translation="MEILKKTGAFREGHFVYPSGKHTPNYFQMPLAFRYYDTARVLAV
                     ALSRLLRVEKDVSSVLPKVAVISPGPGGIPVAFGVREALSAEQIYWAEREEGKRMFRQ
                     YINKGEINPCIIVDDIIRSGDAIREAVALVEELGATVIGCATIVRFTDALDKINDDIA
                     IKSLVEFDSKFYDSSEECVGCKKGAPAEHVRF"
     gene            complement(822..1301)
                     /locus_tag="VGB76_14130"
     CDS             complement(822..1301)
                     /locus_tag="VGB76_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0100083.1"
                     /translation="MWKKLLGTMWRGAPKSVRRWGVWLAEPRFMVTVGAVVLDDAGRV
                     MLLNHEFRMGSGWGIPGGFMEAGEQPHEALRRELREEIGVELHEVELLGVRTLKRPQQ
                     VEIHFRCRLRASETAAPQSMEINSVGWFDAGALPPGLSQDQRRLIKDALAGGANTAK"
     gene            1534..>2417
                     /locus_tag="VGB76_14135"
     CDS             1534..>2417
                     /locus_tag="VGB76_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100084.1"
                     /translation="MSTNESTRPGAAPQGVAQAAPTEQQASKSGAPKRFEKGQTVCNE
                     KDAKGKLHNGHLKQVSPIREEARQHMRGDDLLYRCQTCGTLYMGPPLGHVRDPRKQAR
                     YVQSELVQILEAAGGTLPAFKRNEAGAWVPASNTTPHAPPPVAAAAQNIKPAAPTPKP
                     ATTPAATPAATNAAAPSGETPEQKRARIIAEAKAKAAARMAGGAIPTSGTTPTSGAET
                     SNAAPPAHANADAPSGETVEEKRARIIAEAKAKAAAKMAAAKGGKRGGEETQVAPPTP
                     PAPSTPAPTQTQTPVPTPS"
BASE COUNT          494 a          804 c          716 g          403 t
ORIGIN      
        1 cagtgacggg tgacaagcga cgagtgacgg tttttctaac cgtcactcgt cgcttgtcac
       61 ccgtcactgc ttttaaaatc tgacgtgctc ggcgggcgcg ccctttttgc agccgacgca
      121 ctcttcgctg gaatcgtaga acttggaatc gaattcgacg agcgacttga tggcgatgtc
      181 gtcgttgatc ttgtccagcg catcggtgaa gcggacgatg gtggcgcagc cgatgacggt
      241 ggcgccgagt tcctcgacga gcgcgaccgc ctcgcggatg gcgtcgccgc tgcggatgat
      301 gtcgtcaacg atgatgcacg ggttgatctc gcccttgttg atgtactggc gaaacatgcg
      361 cttgccttct tcgcgctccg cccagtaaat ctgctccgcg ctcagcgctt cgcgcacacc
      421 gaaggccacc gggatgccgc cgggacccgg actgatgacc gccactttcg gcaacacgct
      481 cgacacgtct ttctcgacgc gcagcaggcg cgagagcgcg acggcaagga cgcgcgccgt
      541 gtcgtaatag cgaaaggcga gcggcatctg aaaatagttc ggcgtgtgtt tgccggaagg
      601 atagacgaag tggccttcgc gaaacgcgcc cgtcttcttc aatatttcca tgacttcatc
      661 ttgtgatgga acgagttgca ccattagctc atgtctccac taagaaaagg atgagggatg
      721 agggatgagg gatgaacagg aatggtcgaa ccgctttggg cagttttctg attcatcctt
      781 catccctcat ccctcatcct taagttttcg acagcatacc actacttcgc cgtgttcgcg
      841 ccacccgcga gtgcgtcttt gatgaggcgg cgttgatctt gagagaggcc gggcggaagc
      901 gcaccggcgt caaaccagcc gacgctgttg atctccatgc tctgcggcgc agccgtttcg
      961 ctcgcgcgca ggcggcagcg aaagtgtatc tccacctgtt gcggtcgctt gagcgtgcgc
     1021 acgccgagga gttccacttc gtgcagttct acgccgatct cctcgcgcag ttcgcgcctg
     1081 agggcttcgt gcggttgctc gcccgcctcc ataaaaccgc cggggatgcc ccacccgctg
     1141 cccatgcgaa actcgtgatt caacagcatc acgcgccccg cgtcgtctaa gacgaccgcg
     1201 cccaccgtca ccatgaaacg cggctcggcg agccacacgc cccaacgccg cacgctcttc
     1261 ggcgcacccc gccacatcgt ccccagcaat tttttccaca tgcgccgcca tgataaacga
     1321 tgaaggatga acgcggaacg ttgaatgaaa gaaactgctt ttgtgttcca ttcaacgttc
     1381 cgcgttcatc cttcatcgtc tcttttcatt gacgcgcttc gtcgtttctc cgtaagattg
     1441 tgcgcaagtt tttgcacgtc tggaaaaaag ccggagcgtg ccgccggacg gttcacaggc
     1501 aggcggcgcg cgagaagtct ggaagcgaaa atcatgtcaa ctaatgagag cacaaggccg
     1561 ggtgccgcgc ctcagggcgt ggctcaagcg gctcccaccg agcagcaggc gtcgaagtcc
     1621 ggcgcgccga agaggttcga gaaggggcag accgtctgca acgaaaagga cgccaagggc
     1681 aagctgcaca acggccatct gaagcaggtg agtcccatcc gcgaagaggc gcggcagcac
     1741 atgcgcggcg acgatctgct ctaccgctgc cagacctgcg gcacgctcta catggggccg
     1801 ccgctcggac acgtccgcga cccgcgcaag caggcgcgct acgtgcagag cgaactcgtg
     1861 cagatactcg aagcggcggg cggcacgctc cccgccttca aacggaacga ggcgggcgcg
     1921 tgggttcccg ccagcaacac gacgcctcat gcgcccccgc ccgtcgccgc cgcagcgcaa
     1981 aacatcaagc ccgccgcccc gacgccgaag cctgccacga cgcccgccgc gactccggca
     2041 gcgaccaacg ccgccgcgcc gtcaggcgag acgcccgaac agaaacgcgc acgcattatc
     2101 gccgaggcca aagccaaagc cgccgcacgc atggcaggcg gcgcgatacc gacgagcggc
     2161 acgacgccga cgagcggcgc agagacatcc aacgccgcgc cgcccgccca cgcaaacgcc
     2221 gacgcaccct ccggtgaaac tgtcgaagag aaacgcgcgc gcatcatcgc ggaagcgaag
     2281 gccaaagccg ccgccaagat ggccgccgcg aagggcggca agcgcggcgg cgaagagaca
     2341 caggttgcgc cgcccacgcc gcctgcacct tccacacccg cgccaacaca aacgcaaacg
     2401 ccagtgccaa cgccgtc
//
LOCUS       DASWNS010000403         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_300716, whole genome shotgun sequence.
ACCESSION   DASWNS010000403 DASWNS010000000
VERSION     DASWNS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300716"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VGB76_14140"
     CDS             <1..230
                     /locus_tag="VGB76_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100085.1"
                     /translation="QDYSLGVAYRPLLINNITMTFGASTFVPGRGFRDIYTDKSRNCP
                     PNVEDYCASDRTIIDPSKPLFSLFAQLKFIF"
     gene            277..>3482
                     /locus_tag="VGB76_14145"
     CDS             277..>3482
                     /locus_tag="VGB76_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100086.1"
                     /translation="MQGVGKADRIKLVIFLFFIAAISLTLLGGARSSAQQRHSAPPQP
                     PQLARAASSERDDQPAPAAKPDASVAPAKIHDNAANVSQTAAAATEATEGCVACHNNI
                     EPMHVTSSGKLEAGGKDGQGLSCTYCHGGNPAGKTTEESHVRPRHPDIWKVGGKASSA
                     NPERTNALLNRESWEFVRFINPADLRVTSKTCSECHAPEVAANDNSMMRHGAMLWGAA
                     LYNNGGFPLKDARFGEAYAEETGAPLRLIQVPQPTKEEQLLKGWLPFLDPLPRWEISQ
                     PGNLLRVFERGGRRRLEVGLPDREEEPGKPDKGLSARGLGTQQRTDPVYLGIQKTRLL
                     DPTLNHLGTNDHPGDYRSSGCTACHVVYANDRSPVNSGPYARYGNMGTTATADTNIPR
                     NEPGHPIKHQFTNAIPTSQCMTCHMHPGTNMVATYTGFMWWDNETDAGKMFPAKQVTP
                     SQDEEQEKFDRNPEGAHVRGLWSMRKWKDRASPEDFLVKTGEPGGEFGKQLKQTAFAD
                     YHGHGWMFRAVFKRDRAGNMLDGYGKQIKTVTPKKLADAVAFSGDGAYKSGQPVHLKD
                     IHLEKGMHCADCHFRQDNHGTGILYNEPRAAVEINCTDCHGTIREKAKLITTGYAAGQ
                     GIEGGKLVDKERGRNLRAPRSGNKDISRNLYARDETGAKIPLFELAPAAGLKKKVKTE
                     DGREVTIDIKAGEIVQNSIVEPGKWWRVKQTIDSIDPKSPDYNELSRYAKTIRKDNST
                     WGDVPEKDESLAHADNSMTCFSCHSSWMTSCFGCHLSMKANRKMPNRHNEGDDSRNYT
                     TYNFQVLRDDVFMLAKDGTVTGHRVAPAASRSAVIVSSQNQNREWIYSQQQTISTEGF
                     SGQSFSTHVPHTVRATETKGCTDCHVAKENNNNAWMAQLLMQGTNFVNFMGRYVYVAA
                     EHSLEVVPVTEREDPQAVFGSTLHKIAYPDNYKKFVDGGRVLKEYYEHVGNPEVLQVQ
                     MRGEYAYVAAGEGGLRIYDIAQIDHKGFSERITTAPVSRFGQKFWVKTKYATAVAAPS
                     TLALDPLRTRRPENQEQPIHPLYGYLYVADRHEGLVLFG"
BASE COUNT          779 a         1157 c          994 g          552 t
ORIGIN      
        1 ggcaggacta cagcctcggc gtcgcctatc gcccgctcct gatcaacaac atcacgatga
       61 ccttcggcgc gtccacgttc gtgccggggc gcggcttccg cgacatctac acggacaagt
      121 cgcgcaactg cccgccgaac gtcgaagatt attgtgcgtc ggacagaacc atcatcgatc
      181 cgagcaagcc cttgttctcc ttgttcgcgc agttgaagtt catcttctga gtttcatcat
      241 ttatcccggc agttatcgtg agacggagaa tttagaatgc aaggtgtcgg taaagctgat
      301 cggatcaagc tggtcatctt cctatttttc atcgcggcca tcagcttgac gctgctcggc
      361 ggcgcgcgtt cgtccgcgca acagcggcac agcgcgccac cgcaaccgcc acaactcgcc
      421 cgcgccgcat cctctgaacg cgacgatcaa cccgcgcccg ctgcaaagcc ggacgcgagc
      481 gtcgcgcctg cgaagattca cgacaacgct gccaacgtct cgcagaccgc cgccgccgca
      541 acagaggcga ccgaaggctg cgtcgcctgt cataacaaca tcgaaccgat gcacgtcacg
      601 tcgagcggaa agctcgaagc gggcggcaag gacgggcagg gactctcctg cacctattgt
      661 cacggcggca accctgcggg caagactacg gaagagtcac acgtgcgccc gcgccacccg
      721 gacatctgga aagtcggcgg caaagcgtcg agcgcgaacc cggagcgcac gaacgccctg
      781 ttgaatcgtg agagttggga gtttgtgcgc ttcatcaacc cggcggattt gcgcgttacg
      841 tcgaagacct gctcggaatg tcacgcgccg gaagttgcgg ccaacgacaa cagcatgatg
      901 cgccacggcg cgatgctctg gggcgcagcg ctctacaaca acggcggctt cccgctcaaa
      961 gacgcgcgct tcggcgaagc ctacgccgaa gagacgggcg cgccgctacg ccttattcaa
     1021 gtgccgcagc cgacgaaaga agagcaactt ctgaaaggct ggctgccctt cctcgatccc
     1081 ctcccgcgct gggagatttc gcaaccgggc aacctgttgc gcgtcttcga acgcggcggc
     1141 aggcgtcgtc tcgaagtggg cttgcccgac cgcgaagaag aacccggcaa gcccgacaaa
     1201 ggactgagcg cgcgcggcct cggcacgcag caacgcaccg atccggtcta tctcggcatc
     1261 cagaagacgc gcctcttaga cccgacgctc aatcatctcg gcaccaacga tcatcccggc
     1321 gattatcgct cctccggctg cacggcctgt cacgtcgtct atgccaacga ccgcagcccc
     1381 gtcaactccg gtccttatgc caggtacggc aacatgggca cgaccgccac cgccgatacc
     1441 aacatcccgc gcaacgaacc cggccacccg atcaagcacc agttcaccaa cgccattccc
     1501 acttcgcagt gcatgacctg tcacatgcac cccggcacca acatggtcgc cacctacacg
     1561 ggcttcatgt ggtgggacaa cgagacggat gcgggcaaga tgtttccggc caaacaggtc
     1621 acgccgtcgc aggatgagga gcaggagaag tttgaccgca accccgaagg cgcgcacgtg
     1681 cgcggcctct ggtcgatgcg aaagtggaaa gaccgcgcct cgcccgaaga ctttctggtg
     1741 aagacgggcg aaccgggcgg cgagttcggc aagcaactca aacagacggc ctttgcagac
     1801 tatcacggtc acggctggat gttccgcgcc gtcttcaaac gcgaccgcgc gggcaacatg
     1861 ctcgacggct atggcaaaca gatcaagacc gtcaccccga aaaaactggc cgacgccgta
     1921 gccttctccg gcgacggcgc gtacaagtca ggccagcccg tccatctcaa ggacatacac
     1981 cttgagaagg gcatgcactg cgccgattgc cacttccggc aggacaatca cggcacaggg
     2041 attctctaca atgaaccgcg cgccgccgtc gagatcaact gcaccgattg tcacggcacg
     2101 atccgcgaga aagcgaaact catcaccacc ggctacgcag ccgggcaagg catcgaaggc
     2161 ggcaaactcg tggacaaaga acgcggtcgc aacctgcgcg cgccgcgcag cggaaacaaa
     2221 gacatctcgc gcaatctcta cgcacgcgac gagacgggcg cgaagattcc gctcttcgaa
     2281 cttgcgcccg ccgccggtct gaaaaagaag gtcaagaccg aggacggcag agaagtcacg
     2341 attgacatca aggcgggcga gatcgtgcag aactccatcg tcgagccggg caaatggtgg
     2401 cgcgtcaagc agacgataga cagcattgat ccgaagtcgc ccgactataa cgaactctcg
     2461 cgctacgcca agaccattcg caaggacaac tcgacgtggg gcgacgtgcc ggaaaaggac
     2521 gagtcgctcg cgcacgccga caactcgatg acgtgcttct cctgccactc ttcgtggatg
     2581 acgagttgct tcggctgcca cctctcgatg aaggccaacc gcaagatgcc caaccgccac
     2641 aacgagggcg acgactcgcg caactacacg acctacaatt ttcaggtctt gcgcgacgac
     2701 gtgttcatgc tcgcgaaaga cggcacggtg acgggtcatc gcgttgcgcc cgccgcgtcg
     2761 cgctccgccg tcatcgtcag ttcgcagaac cagaaccgcg agtggatcta ttcgcagcag
     2821 cagaccatct cgacggaagg tttcagcggc cagtcgttct cgacgcacgt gccgcataca
     2881 gtccgcgcga cggagacgaa gggctgcacc gattgccacg tcgccaagga gaacaacaac
     2941 aacgcatgga tggcgcaact gctcatgcag gggacgaact tcgtcaactt catgggtcgc
     3001 tacgtctatg tcgccgccga gcactcgctc gaagtcgtcc ccgtcaccga acgcgaagac
     3061 ccgcaggccg tcttcggcag caccctgcac aagatcgctt atcctgacaa ctataagaag
     3121 ttcgtggatg gcggtcgcgt gttgaaggaa tactacgagc acgtgggcaa cccggaagtc
     3181 ttgcaggtgc agatgcgcgg cgagtatgcc tatgtcgcgg cgggcgaagg cggactgcgc
     3241 atctacgaca tcgcgcagat cgatcacaaa ggcttctccg agcgcatcac gaccgcgccc
     3301 gtctcgcgct tcggccagaa gttctgggtc aagacgaagt acgccacagc cgtcgccgcc
     3361 ccctcgacgc tcgcgctcga cccgttgcgc acgcgtcgcc ccgaaaatca ggagcagccg
     3421 attcacccgc tctacggcta cctctacgtc gcagacagac acgaaggctt agtcctcttc
     3481 gg
//
LOCUS       DASWNS010000404         5785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_225188, whole genome shotgun sequence.
ACCESSION   DASWNS010000404 DASWNS010000000
VERSION     DASWNS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225188"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="VGB76_14150"
     CDS             complement(<1..1697)
                     /locus_tag="VGB76_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100087.1"
                     /translation="MKQVVTPEIVQKVLATIRKRQADYDYFFSQLNSPVWLKPLKEAG
                     MFSTPPPPEVEGQYISFPRWPESEYLARMAATAPDAVLEVVLQIPDTENIRVHDDLVG
                     TALAMPADLAAKLIDRAKKWAESSYHPLFAEKLGSLVAHLAQGGQVDEALGLARVLLA
                     VLPDPRRREQDDEDDTYRFPPTPQPRFNTWDYRIILKKNIPSLVDAAGGRALTLLCDL
                     LEEAMRLSRNRSDSPGPHDHSYIWRRNIDSDSHDDEVRDLLISAVRDATDRIANRDSQ
                     EVLGMILMLEARPWLVFRRIALHLLRHFPEAGANLIAERLTDPSLFGVLELWNDFYLL
                     ARDHFARLTPGQQDVYLSWVESGRDPESFETPEERRYWQLRRLAPIKDALPDDWKERY
                     NELVREFGEIEWPEYVSPPSSMHFGWESPKSSEDLSQMSFDELITYLKEWQSSGSPVG
                     PSPEGLGQQLTAVVASDPERFADEVEALKGLDPTYVRAVISGLHEALKQKGTVAWPPI
                     LRLCKWVIEQPQETNKPKSSYAERDPDWNWTKGSIQNLLEEGLKSDDAGIPIDLRNTV
                     "
     gene            complement(1675..2805)
                     /locus_tag="VGB76_14155"
     CDS             complement(1675..2805)
                     /locus_tag="VGB76_14155"
                     /inference="COORDINATES: protein motif:HMM:NF024668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEY0100088.1"
                     /translation="MADRDSVVVSSTIRDLPAHREEVRDACLRLGMYPLMMEHIPASA
                     ADAIRESLRLVNQANIYVGVFAHRYGYIPPGYEISITEMEYNRAVERNIDRLIFLMGD
                     DHPITIGDVEFGDGTGKLGLFKDRLKVEKVVNFFKSPEQLGARVIHALSQYRRPAFEP
                     FQLVGPQQILYEALVAIGPGLGDMYLGALMAFERRETADRYAQAAHSLRELMEKLPKY
                     LDLPVEKKPPSLKEKVRALSQQRDRTVKNSNSRDGSVWRGEIDDHLHRYLTDSDEFFQ
                     WFSEEHLTRRRRTVKVLRGLDPSSLQLPEPLEDSNVKQWEKCHGYFQGVSHHTVTGVE
                     EAFREQLSVLESFLLDRIAPRTSEDFASIDDLIREVEAGGNS"
     gene            complement(3024..4346)
                     /locus_tag="VGB76_14160"
     CDS             complement(3024..4346)
                     /locus_tag="VGB76_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100089.1"
                     /translation="MDFRNETLYAAIRAYVAAAIEFIARKAEQEASRLSISQEDDYFA
                     HCWTHALYESDLSEVPEYGAALRELCGDLEISRQLDANVGSYNRGTHTPSAARMMRYA
                     LGLGRREEQYVFNPGRFDVEYSAFEEIFYNDRMRCVAVAPLQGLVMEDAVVPLSNGLE
                     VRRLIKEEMAPYRTPGSVWEDEWCAVRATYEIPKLVGTGGEDRFKAMTLEERNKGRKK
                     EDAAEEEANDRIEDVINALRLYGMFTVYHSGIMHLTPKWLFIGSHSVPNRVLGERMIT
                     YQFGSEGDVRFFPPFWSKLENPKVKKELGTAVRRFSYSCVRHLNEDKIIDLMIAAEAL
                     FLRGTREGEKRFRLAFRAGLFLGADQAARKEVYNRMKKAYDLRSTLVHGGSNPSLRRS
                     RGEITENDKLIGAVGDDVRNGILKAIEILSAPDAVILDDAYWDKLTFI"
     gene            complement(4604..5530)
                     /locus_tag="VGB76_14165"
     CDS             complement(4604..5530)
                     /locus_tag="VGB76_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0100090.1"
                     /translation="MAVWSDATTTTSSPRRRDIQRDKRNAVASFFAHAGKHLPDIAPL
                     DVKSWQEALEAKGLAPTTVYYRLCHLSSFFAWAQRHPSLGRYVTSNPVRSAHPKAPKP
                     YQTESVSALTDEEFAALLSVVRGKAASDDVVGKRDYALLLFYVATGLRRAEVINLRGR
                     DITFSGEALILRSRVKGGDYVGREVRDPSVGAALHDYLNASDRAAALSSDAPLWTRHD
                     HAGRPGAPLTSHAFVLNLKRYARRAGIGSIHLHQTRHTFARMVAEDSGSITETQDALG
                     HKNVATTRVYVQRIAVRRDRHSERILKRFDAE"
BASE COUNT         1142 a         1703 c         1627 g         1313 t
ORIGIN      
        1 actgtattgc gaagatcgat tgggattccc gcgtcatcgg atttcagccc ttcctcaagg
       61 aggttctgga tggagccttt agtccagttc caatctgggt cgcgttcggc atatgagctt
      121 ttgggtttat tagtttcctg aggctgttca attacccact tacacagtct caagatcggc
      181 ggccaagcga cagtgccttt ttgcttcagt gcctcgtgga gtcctgaaat cactgcgcgc
      241 acgtacgtcg gatcaaggcc tttgagggct tcgacctcgt cggcgaagcg ttcgggatct
      301 gaagcgacca ctgccgtgag ttgctgcccc agcccctcgg gcgacggccc tactgggctt
      361 ccagaagact gccactcttt aagatatgtg atgagttcgt caaagctcat ctgacttaaa
      421 tcttcagagc ttttcggact ctcccaacca aagtgcatgg acgacggggg tgagacgtac
      481 tcgggccact ctatctcccc gaactccctg actaactcgt tatagcgttc cttccagtcg
      541 tcagggagag cgtctttaat aggcgccaga cgtcgcaact gccaataacg tcgctcttcg
      601 ggcgtctcga aactttccgg gtcacggcca gattcaaccc agctaaggta cacgtcctgc
      661 tgcccaggcg tgagacgggc gaaatggtcc cgcgcgagta aatagaagtc gttccaaagc
      721 tctaacacac cgaagagcga aggatcagta agcctctcag ctatgagatt agccccggcc
      781 tcagggaagt gccgaagtag gtgaagagcg atgcggcgga agaccagcca cggtcgcgcc
      841 tcaagcatta ggatcattcc gaggacttcc tgtgagtctc tgttagcgat ccgatccgtc
      901 gcgtcgcgga ctgccgagat gagcaaatct ctaacctcat catcatgaga gtcactatca
      961 atattgcgcc tccagatgta tgaatggtca tgcggcccgg ggctgtcgct gcgattccgg
     1021 gaaagacgca ttgcctcttc taacaggtca cagagcaatg tcagggcccg cccgccggct
     1081 gcgtcgacga gcgaaggaat gttcttcttg agaattatcc ggtaatccca cgtgttaaag
     1141 cggggctgcg gcgtaggcgg gaagcggtag gtgtcgtcct cgtcgtcctg ctcccgcctt
     1201 ctcgggtccg gcaacaccgc aagcaggact cgggccagac ctaaagcttc atccacctgc
     1261 cccccctgag cgagatgtgc caccagcgaa cctaactttt cggcgaaaag aggatggtag
     1321 gatgattccg cccacttctt tgccctatca ataagtttcg ccgcaaggtc cgcgggcatc
     1381 gcgagggcgg tgccaacgag atcatcatgg acgcgaatgt tctccgtgtc cggtatctga
     1441 agcaccacct caagtaccgc atccggcgcg gttgctgcca tccgggctaa atactccgac
     1501 tcgggccaac gaggaaagct gatgtactgc ccttccacct cgggcggggg aggtgtgctg
     1561 aacataccgg cttctttaag aggtttgagc cagacagggc tattcaactg gctgaagaaa
     1621 taatcgtagt cagcctgacg tttcctgatc gtggctaaaa ccttctgaac tatctcagga
     1681 gttaccacct gcttcaacct ctcttatcaa gtcatcgata ctagcgaaat cttcggatgt
     1741 ccgaggtgca atcctgtcca gcagaaaact ttccaggaca gaaagctgct cccggaaagc
     1801 ttcttccaca ccagtgactg tgtgatgcga caccccctgg aagtagccgt gacacttctc
     1861 ccactgctta acattcgagt cttcgagcgg ctccggaagc tgaagcgacg acggatctaa
     1921 tccccgaagt acctttaccg tccgtcgcct cctggtcagg tgttcctcgg aaaaccattg
     1981 gaaaaactcg tccgagtccg tgaggtacct gtggagatga tcatcaatct ccccgcgcca
     2041 gaccgagccg tcacgactat tcgagttttt aacagtcctg tctcgctgct gggagagggc
     2101 acgaactttt tcctttaggc tcggcggttt tttctcaacc gggaggtcga ggtatttagg
     2161 cagcttttcc atcagctcgc gcaggctgtg tgcagcttgc gcgtatcgat cagccgtctc
     2221 cctgcgctcg aacgccatca gtgctccgag gtacatgtcg cctaacccgg ggcctatcgc
     2281 gactaaagcc tcgtagagaa tctgctgcgg cccgactaac tggaatggct cgaaggcggg
     2341 tctgcggtat tgtgaaaggg catgaattac gcgggcgccc agttgttctg gggacttgaa
     2401 gaagttaacg accttctcga ctttaagccg gtctttgaat agacctaatt tccccgtccc
     2461 gtcgccgaac tcgacgtcgc cgattgtgat cgggtgatca tcacccatga ggaagatgag
     2521 gcgatcaatg ttgcgctcga cggcccggtt atactccatc tcggtgatag agatttcgta
     2581 ccctggaggt atatagccgt aacggtgcgc aaagactccc acataaatat ttgcttgatt
     2641 tacaagcctg agtgactcac ggattgcgtc ggcggcgctg gcgggtatgt gctccatcat
     2701 cagcggatac atccccagcc tcaagcaggc gtcccgcacc tcctcacgat gcgcaggcag
     2761 atcgcgaatg gtgctgctga ccacaacact gtcacgatcc gccacgttat ttatcctcct
     2821 atcaatttct gctcttcagg gtttgtcttg gctttcggta aaatagtttc ccacaaaact
     2881 aacatggctc acaaagttac cctgccgcgg tcgcgcgtgg agagctagac agacgttgac
     2941 ggtataccat gacggggtgg ttgatgagct tcagcgcatt gggtgcaggt atagggcaaa
     3001 cgaccggtgg ggtcaaaatc aggtcaaatg aatgtcagct tgtcccagta ggcgtcgtcc
     3061 agaatgaccg catctggtgc cgagagaatc tcaatggctt taaggattcc gttgcgcacg
     3121 tcgtccccga ccgcccctat gagcttatcg ttctcggtaa tctcccccct cgaccggcgt
     3181 aaggacgggt tgctaccgcc gtgcaccagc gtgctcctaa ggtcgtacgc ctttttcatc
     3241 cggttataga cttccttccg agccgcctga tctgcgccga gaaagaggcc agcccggaag
     3301 gccagacgga agcgcttttc gccttcccta gtgccgcgca gaaagagggc ttcggcggcg
     3361 atcatcaagt cgatgatctt atcctcgttc aggtgccgca cgcatgagta actgaaacgg
     3421 cgcacggccg tgcctagctc tttcttcact ttagggtttt cgagcttact ccagaacggc
     3481 gggaagaacc gcacgtcgcc ttccgagccg aactggtaag taatcattct ctctcccaga
     3541 actctgttgg gcacactgtg ggagccgatg aacagccact taggcgtcag gtgcattatg
     3601 ccggagtggt agacggtgaa catgccgtac agcctgagcg cgttgatcac atcttcgatg
     3661 cggtcgttgg cctcttcctc ggcggcgtcc tcctttttcc tccccttgtt gcgctcttcc
     3721 agagtcatcg ccttgaagcg atcctcacct ccggtgccga cgagcttggg gatttcgtat
     3781 gtcgccctta cggcgcacca ctcatcctcc cagaccgagc cgggcgtgcg gtacggtgcc
     3841 atctcctcct ttatgagtcg tctgacctca aggccgtttg agagggggac gaccgcgtcc
     3901 tccatcacga gtccttgtag gggcgcgacg gctacgcacc gcatgcggtc gttgtagaag
     3961 atttcctcga aggctgagta ttcgacgtcg aagcgtccgg ggttgaaaac gtattgttcc
     4021 tcgcgcctcc cgaggccgag ggcgtacctc atcatcctcg ccgccgaagg cgtgtgcgtt
     4081 cccctgttgt aggaccccac gtttgcgtcg agctgcctgc ttatctccag gtcgccgcaa
     4141 agctcgcgca gagcggcccc gtactcaggc acctccgaga ggtcactctc gtagagcgca
     4201 tgcgtccagc agtgggcgaa gtaatcatcc tcctgagaga tggatagcct cgacgcttcc
     4261 tgctcggctt ttcgggctat gaattcgatg gccgcggcga cgtaggccct aatggcggcg
     4321 tacaacgttt cgtttctgaa atccatagaa acttgccgga ccttgtcccc tgcggtgggt
     4381 ggttgcgccg gcagccccct atagtctgta gcaatgaaac ttctaatttg cgaggcggtt
     4441 gtaaacgtct aggtagcggt aattcttatg tcggcccagc aggtcaatca aatccagcac
     4501 ctcctgcttt acttcttccg cataagtcgc cgttttgatg atcttatccg cgtctttatc
     4561 accgaagaat gcccagggtg gcgcgtgatt ctgccggtgg tatttattcc gcgtcgaaac
     4621 gcttcaatat ccgctcgcta tggcggtcgc gccgcacggc aatgcgctgc acgtagaccc
     4681 tcgtcgtcgc gacgttctta tgccccagcg cgtcctgcgt ctcggtgatc gagccggagt
     4741 cttcggccac catgcgggcg aatgtgtggc gcgtctggtg taggtggatg ctgccgatac
     4801 cggcccggcg ggcgtatcgc ttcaaattca agacgaacgc gtgcgacgtg agcggcgcgc
     4861 cgggccgccc agcatggtcg tgtctggtcc acagcggggc gtcgctcgac agagcggccg
     4921 ctctgtcact cgcgttcaga taatcatgca gcgcggcgcc gacactaggg tcgcgcactt
     4981 ccctgccgac gtaatctccg cccttaactc ggctgcggag gatcaacgct tcaccgctaa
     5041 aggtgatgtc tcgaccgcgc aggttgatga cctctgcacg cctcaagccc gtcgcaacgt
     5101 agaagagaag gagcgcgtaa tctcgcttgc cgacgacatc atccgaagcg gccttacccc
     5161 tgactaccga gagcagcgcg gcgaactcct cgtcagtgag ggcgctaacg gactcggtct
     5221 ggtaaggctt cggggctttg gggtgcgccg acctgaccgg gttggaggtg acgtacctcc
     5281 cgagagacgg gtgccgctgc gcccaggcga agaaagagga aaggtggcac agccggtagt
     5341 agacggttgt tggtgctagc cccttcgcct ctagagcctc ctgccacgac ttcacgtcga
     5401 gcggggcgat gtccggcaaa tgtttccccg catgggcgaa gaatgaggcg accgcgttcc
     5461 gcttgtcccg ctggatgtcg cgccggcggg ggctggaggt cgtggtcgtg gcgtcggacc
     5521 aaaccgccac cgcgttgcgg atggctgtcg agtcttgatc gtcggctctg acaagagccg
     5581 catgctcatc ccctacggtc tgaccttcgt tcataactgg agagaatcag aaatcagtgt
     5641 aagtagtgtc gctggcggta ctcccctttc gcgccgccgc gcggatttcg tcctggctcg
     5701 ccggcggggt cgtggcgcct cttcgcacgt aataacgcag gtttctccga tccgaaccta
     5761 cgccgtaggg tccactgtct ccctc
//
LOCUS       DASWNS010000405         4410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_508517, whole genome shotgun sequence.
ACCESSION   DASWNS010000405 DASWNS010000000
VERSION     DASWNS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508517"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2775)
                     /locus_tag="VGB76_14170"
     CDS             complement(139..2775)
                     /locus_tag="VGB76_14170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY0100091.1"
                     /translation="MAIEITSERREARRLRWIWQPERREDERLRRASRRMRVLRLGAV
                     LSVVLCAAAVVSIIYCYNDAAGKIDQRLASGYLTSRAGLYAAPRVLREGQSFSRESLV
                     ETLRRAGYVEQSAGEQSASDVWSGAFRTVEGGIEISPRRMQAEHAPEGFTPEVVRVSF
                     DRKGRIGEITGDGVRLDAFTLEPEALTTDATTKTGTRATLAYTDIPPVLVNAIRAIED
                     RRFFEHHGIDPTGIGRALLSWTGDGSGRAPEHQGGSTITQQLVKNTYLTPERTLTRKF
                     NEALLAAALERRLSKEDIFALYCNEIYLGQRGAFAVRGVKQAARVYFGKELKDLSLAE
                     AATIAGMIQSPARYAPDRHAEASRARRNAVLASMLRDNSINAEEAKNASNENVNVAPV
                     DPALNATAPYFVDYVNRAVESRLQQASGGVGADERSLRIYTTIDLELQQLAEQAVARQ
                     LTQLEKVYRKRGAQPQAALVALDPNTGHVLAMVGGRNYAESQLNRATDARRQPGSVFK
                     PFVYAAAIESGISPVRLFADAPRAFAFDRHSPAYRPANYGGGYSMRDVTMRTALTKSL
                     NVVTVDLAMQTGLPRVAALAEKFGLQKAAAYPSLALGTSEATPLEIASAYTAFAGNGT
                     RLRASAMTRAVDGEGFKLFEEFLPSAEPVVQPATAYMITDMLTNVIADGTARAARGML
                     KLSAVAGKTGTSRDGWFVGYTPNLVCVVWIGFDDNEQLGLTGAESALPVWSEFVRGAV
                     ELRPELGGAAFLRPESVVSIDIDPASGQLASPSCPVREHVAITRALAPTSECFTHQTL
                     SPLLAASAPQPLAPQSNIAPPDDSTRAVASTPTPASHKTTAPVVVLPAPAIQTTRSEP
                     AHGRRARLTNDLQVVSERAVKQ"
     gene            complement(3051..3236)
                     /locus_tag="VGB76_14175"
     CDS             complement(3051..3236)
                     /locus_tag="VGB76_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100092.1"
                     /translation="MKELGNTVAQLFFIIMVMIALAVSTAALSQWANSDTLGASDNSR
                     IEQGETAAPSLSASATQ"
     gene            complement(3479..>4410)
                     /locus_tag="VGB76_14180"
     CDS             complement(3479..>4410)
                     /locus_tag="VGB76_14180"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0100093.1"
                     /translation="PRRLRVGLALSGGAARGVAHVGVLKVLLEAGVPIDSVAGTSAGA
                     LVGGAFAAGMSVAELEALGREMRWRNFGRMTLSRLGVQSNARMEEYIRERFPVTRFEE
                     MLIPFAVVATDLHTGQAVVMRDEGDAAFAIRASCAVPGWYVPVTDAEGRQLVDGGLVA
                     NLPAAAARSLNPDIVIAVDVNFEGAKFLGPPQSAIGVLIQSIMVVQRTVAAHEGRNAD
                     IVIRPKVGHIRWDEMTRAPEMLAAGEEAARESLAGIHALLAPPIEEHRNWIQRLLHRP
                     PNAADASAVERSDRRELVAPHPREDGNPRSSTR"
BASE COUNT          740 a         1348 c         1458 g          864 t
ORIGIN      
        1 taaaagccgt gtggagggca agaagtgaca gccgcaaaag cggcgacagg cagcagcccc
       61 tagcttaggc tgagggctgt tgcctgtcgc cgcttttgcg gctgacggct tttacttcac
      121 gtctgtcatc ttctgcgctt actgtttcac agcgcgttcg gagacgactt gcaggtcgtt
      181 tgtgagtcgt gcgcgccgcc cgtgcgctgg ctcgcttcgc gtagtctgaa tcgcaggcgc
      241 ggggagaact acgacgggcg cagtcgtttt gtgagacgca ggtgttggcg tcgaagcgac
      301 ggcgcgcgtg gagtcatctg gcggcgcgat gtttgattgc ggcgcgagcg gctgcggggc
      361 ggaagcggcg agaagcgggg agagcgtctg gtgcgtgaag cactcggagg ttggggcgag
      421 tgcgcgtgtg atggcgacgt gttcgcgcac ggggcaagag ggcgaagcga gttgtccgct
      481 cgccgggtcg atgtctatgg agacgacgct ttcgggacgc aggaaagccg cgccgccgag
      541 ttcgggacga agttcgactg cgccccgaac gaattccgac cagacgggca gcgcgctctc
      601 cgcgcccgtc aagccgagtt gctcgttgtc gtcgaaacct atccagacga cgcagacgag
      661 gttcggcgtg tagccgacga accagccgtc gcgcgaggtg ccggtcttgc ccgcgaccgc
      721 agagagtttc aacatcccgc gagccgcgcg tgccgtgccg tccgcgatga cgttcgtgag
      781 catgtccgtg atcatgtagg ccgtcgcagg ctggaccacc ggctcggctg aaggaagaaa
      841 ctcttcaaac agtttgaagc cttcgccgtc aacggcgcgc gtcatggcgg aggcgcgcag
      901 gcgcgtgccg ttgcccgcga acgccgtgta ggccgaagcg atctccaacg gcgtcgcctc
      961 cgacgtgccg agcgcgagcg aaggataggc cgccgctttc tgcaatccga atttttcggc
     1021 cagcgcggcg acgcgcggca aacccgtctg catggcgaga tcaaccgtca cgacgttcag
     1081 agatttcgtc agcgcggtac gcatcgttac gtcgcgcatc gaatagccgc cgccgtagtt
     1141 ggccggacga taagcggggc tgtgtcggtc gaaggcgaag gcgcgcggcg catctgcgaa
     1201 gaggcgaacg ggcgagatgc cgctctcgat ggctgccgca tagacgaaag gtttgaagac
     1261 tgagccgggc tgtcggcgcg cgtcggtggc gcgattgagt tgcgactcgg cgtagttgcg
     1321 gcctccgacc atcgcgagca cgtggcctgt attcggatcg agtgcgacga gcgcggcttg
     1381 cggttgtgcg ccgcgcttcc ggtagacttt ttcgagttgc gtgagttgtc gtgcgacggc
     1441 ctgttccgcg agttgctgca attcgagatc gatggtcgtg tagatgcgca ggcttctctc
     1501 gtccgcgccc acgccgcccg atgcctgctg tagccgcgat tcgacggcgc ggttgacgta
     1561 atctacgaag taaggcgcgg tcgcgttcaa cgccgggtct acaggagcga cgttgacgtt
     1621 ctcgttcgac gcgtttttcg cttcctccgc attgatcgaa ttgtcgcgca gcatcgaggc
     1681 gagcaccgcg ttgcggcgtg cgcgcgaggc ttccgcgtgg cggtcaggcg cgtagcgcgc
     1741 gggactctgg atcattccgg cgatggtcgc cgcttcggcg agcgagaggt ctttcaactc
     1801 tttcccgaaa tagacgcgcg ccgcctgctt gacgccgcgc acggcgaacg cgccgcgctg
     1861 gccgagataa atctcgttgc aatagagcgc gaagatgtct tctttcgaga ggcggcgttc
     1921 gagcgcggcg gcgagcagag cttcgttgaa cttgcgcgtg agcgtgcgtt ccggcgtgag
     1981 ataggtgttc ttgacgagtt gctgcgtgat ggtcgagccg ccctgatgct ccggcgcacg
     2041 cccgctaccg tcgcccgtcc aactgagcag cgcacgcccg atgcctgtcg ggtcaatgcc
     2101 gtgatgctcg aagaagcggc ggtcttcaat ggcgcgaatc gcgttgacga gtacgggcgg
     2161 gatgtccgtg taggcgagcg tggcgcgcgt gccggtcttc gtcgtggcgt cggtggtcaa
     2221 ggcttccggc tcaagcgtga aggcgtcgag ccgtacgccg tcgcctgtga tctcgccgat
     2281 gcgccccttg cgatcaaacg agacgcgcac gacttcgggc gtgaagcctt ccggcgcgtg
     2341 ctccgcctgc atgcgacgcg ggctgatctc aatgccgcct tcaaccgtgc gaaacgcgcc
     2401 gctccacacg tcgctcgcag actgttcgcc cgcagactgt tcgacgtaac cggcgcggcg
     2461 cagagtctcg acgagactct cgcgcgaaaa actctgccct tcgcgcagca cgcgcggcgc
     2521 ggcgtagagt ccggcgcggc tcgtcaggta accggaagca aggcgttggt caattttgcc
     2581 tgccgcgtcg ttgtagcaat agatgatcga aacgaccgct gccgcgcaca agacaacaga
     2641 gagcacagca ccgaggcgca ggacgcgcat ccgccgggag gctcgacgca gacgctcatc
     2701 ctccctgcgc tccggctgcc atatccatct caaacgcctc gcctcgcgtc gctccgatgt
     2761 gatctcgatt gccatgattt aaaagttgtt cagtggttag cggtcagttg ccaattgttc
     2821 tctggccgtc gtcagtcgtt cgattccggc tttaacaact cgctcataca gtttcactgc
     2881 cgatgggcta cgggagaaac cagacaagtt ttttgcacac acgtagccct gtcttcgaga
     2941 ctctttcgcg tccgtgctct ggcttcagcg actctgtaat gtgatcagta aacgactaat
     3001 cggcggcgaa ttctttgaac gagttcgcag tccggcgacg ccttcgagtt ttattgcgtc
     3061 gccgacgcgc tcaaggacgg cgcggcggtt tcgccctgct ctatacggct gttgtcgctc
     3121 gcgccgagag tgtcggagtt tgcccactgg gagagggctg ccgtcgagac ggcaagcgcg
     3181 atcatgacca tgatgatgaa gaataactgt gcgactgtgt ttcctagttc tttcatgccc
     3241 gcctcacctt ctctgaaaat cttccgctgc aagtgttgag caatgtttgt gccagcacga
     3301 agaccgaggc cgccgcacct gtccctgcgg gcgcgtatac tctgaactgt taagagttta
     3361 gcgtggcgac gcgtggactt tggagatgct gcgcgggttt atttgaacga agatgcgtcg
     3421 tctttcggta cgccgcttcg ggaggcgcgg cgagaatttg cgtctcaaaa caattcattt
     3481 agcgggttga cgagcgaggg ttgccgtctt cgcgtgggtg tggggcgact agttctcgcc
     3541 tgtcgcttcg ctcgacggct gaggcgtcgg cggcgtttgg cggtcggtgc agcaggcgtt
     3601 gtatccagtt tcggtgctcc tcgatgggcg gcgcgagcag cgcgtggatt cctgcgagcg
     3661 attcgcgggc ggcctcttcg cccgccgcga gcatctccgg cgcgcgcgtc atctcgtccc
     3721 aacgaatgtg gccgaccttc gggcggatga cgatgtcggc gttgcgccct tcgtgcgctg
     3781 cgacggtgcg ctgcaccacc atgatcgact ggatgagcac gccgatggcc gactgcggcg
     3841 gtcccagaaa tttcgcgccc tcgaaattca cgtccaccgc gatgactata tcggggttga
     3901 gcgagcgcgc ggcggcggcg ggcagattgg cgacgagtcc gccgtccacg agttgccgcc
     3961 cttccgcgtc cgtgacgggc acgtaccaac cgggcacggc gcaactcgcg cgaatcgcaa
     4021 aagcggcgtc gccctcgtcg cgcatgacga ccgcctgccc cgtgtgtaaa tccgtggcga
     4081 cgacggcgaa agggatcagc atctcttcaa agcgtgtgac gggaaaacgc tcgcggatat
     4141 attcttccat gcgcgcgttc gattgcacgc cgagccgcga gagggtcatc cgcccgaagt
     4201 tgcgccagcg catctcgcgc cccagagctt cgagttcggc cacgctcatc ccggcggcga
     4261 atgccccgcc gacgagcgcg cccgccgaag tgcccgccac cgagtcaatc ggcacgcccg
     4321 cctccaacaa gaccttcaac acgcccacat gcgccacgcc ccgcgccgcg ccgcccgaca
     4381 acgccagccc cacgcgcaat cttctaggtt
//
LOCUS       DASWNS010000406         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_300856, whole genome shotgun sequence.
ACCESSION   DASWNS010000406 DASWNS010000000
VERSION     DASWNS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300856"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VGB76_14185"
     CDS             <1..949
                     /locus_tag="VGB76_14185"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0100094.1"
                     /translation="FTGGGQATGRLGLRFDRHVYETTGRLRVTEIVPLSPAALAREIK
                     PGDYLLAVDGREITARTNLDELLNYKINRRVSLTVASGAEGADKRELAIRPVNNGTEK
                     ALLYRKWVEENRAYVSRISNGRLGYVHMFDMGSSSLSQLYVDLDAENHTREGVVVDVR
                     NNSGGFVNVYAIDVLARRGYLTMTPRGQTPAPGRTVLGQRALESPTILVTNQHSLSDA
                     EDFTEGYRTLKLGKVVGEPTAGWIIFTWNQTLIDGSSVRLPRMKITANDGTNMELNPR
                     PVDVPVSRPIGETYTERDSQLETAVRELLRQLDGNRARK"
     gene            974..1498
                     /locus_tag="VGB76_14190"
     CDS             974..1498
                     /locus_tag="VGB76_14190"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0100095.1"
                     /translation="MNERLVWFERRFTLDLPLWMYPNVLERVRGTPARLEDRLLSLPR
                     ELLTKRTANNWSIQEQAGHLLDLGTLDLGRLDDYEARLETLRPADRENRKTHEADHNA
                     DSIERILAEFRAERAEFVRRLDEYDEAFIARTALHPRLNLPMRLIDLIYFIAEHDDHH
                     LARITDLMKVTSDK"
BASE COUNT          346 a          517 c          470 g          286 t
ORIGIN      
        1 cttcacaggc ggcgggcagg cgacggggcg gctcggcctt cgtttcgacc gacacgtcta
       61 tgaaacgacg ggacgcctgc gcgtgacgga gatcgtcccg ctcagtcccg ccgccctcgc
      121 gcgcgagatc aagcccggcg actacctgct cgccgtagac gggcgtgaga tcacggcgcg
      181 cacgaacctc gatgaactgt tgaactacaa gatcaaccgc cgcgtgagcc tgacggtggc
      241 gtccggggcg gagggcgcgg acaagaggga actggcgatt cgtccggtta acaacggaac
      301 ggagaaggct ctgctctatc gcaagtgggt ggaggagaat cgcgcctatg tctcgcgcat
      361 cagcaacggt cggctcggct acgtgcacat gttcgacatg ggatcatctt cgctctcgca
      421 actctacgtt gacctcgacg cggagaatca cacgcgcgag ggcgtcgtcg ttgatgtgcg
      481 caacaacagc ggcggcttcg tcaacgtcta tgcgattgac gtgctcgcgc gtcgcggtta
      541 tttgacgatg acgccgcgcg gccaaactcc cgcgcccggt cgcacggtgc tcggccaacg
      601 cgcgctcgaa tccccgacca tcctcgtcac caatcaacac tcgctctccg acgcggaaga
      661 tttcaccgaa ggttatcgca cgcttaaact cggcaaagtg gtgggcgagc cgaccgccgg
      721 ttggatcatc ttcacctgga accagacgtt gattgacggc tccagcgtgc gtctgccgcg
      781 catgaagatc accgccaacg acggcacgaa catggaactg aacccgcgcc ccgtagacgt
      841 gcccgtctcg cgccccatcg gcgaaactta cacggagcgt gattcgcaac ttgaaaccgc
      901 cgtgcgcgaa ctgctccggc aactcgacgg caaccgcgcg cggaagtaag gcataggaaa
      961 ggagcgcgaa gcgatgaatg agcgcctcgt ctggtttgaa aggcggttca cgctcgacct
     1021 gcctctctgg atgtacccga acgttctaga gcgcgtgcgc ggcacgcccg cgcgtctcga
     1081 agaccgtctc ttatctttgc cgcgcgaatt gttgacgaag cgtacagcca acaactggtc
     1141 aatacaggaa caggccgggc acttgctcga cctcggaacg ctcgacctcg gacggctcga
     1201 cgattacgaa gcgcgcctcg aaacgctgcg ccccgccgac cgcgaaaacc gcaagacgca
     1261 cgaggcggat cacaacgcag actctatcga acgcatcctc gcggagtttc gcgccgagcg
     1321 cgccgagttc gtgcgccgtc tcgatgaata cgacgaagcc ttcatcgcgc gcacagccct
     1381 gcacccgcgc ctgaacctgc cgatgcgcct tatagacctc atctacttca tcgccgaaca
     1441 cgacgatcat catctcgcgc gcatcaccga tttaatgaaa gtgacgagtg acaagtgacg
     1501 ggtgacaagt taaagacaag taaatcgtga atcgtaaaca gcaggaagca ggttttctct
     1561 gttcacgatt tacgattcac ggctcttgct cggcactcgt cacttgtcac tcgtcactg
//
LOCUS       DASWNS010000407         4493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_16302, whole genome shotgun sequence.
ACCESSION   DASWNS010000407 DASWNS010000000
VERSION     DASWNS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16302"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..964)
                     /locus_tag="VGB76_14195"
     CDS             complement(296..964)
                     /locus_tag="VGB76_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100096.1"
                     /translation="MRSFAPLSIALALTILLAPAARGQTAAASVAANGQVSEAVFVSI
                     APGAQLSGETLPLTYSNLDRQTVRLSIDASGIGSDRRINIPLQLRSNVGYTLSAATNL
                     SGATLLRGLCVRSVRATGRHVAGGAVNAVRTATCEDGTAGVQGRNAHRSMKGLSSPAN
                     LLQGQLISLSGTADSPFNALEVLIELEVETQVRWQPQGSIELILSASPRSLASVLMND
                     GRKP"
     gene            1488..4259
                     /locus_tag="VGB76_14200"
     CDS             1488..4259
                     /locus_tag="VGB76_14200"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HEY0100097.1"
                     /translation="MDNSSSIRTAIAAVCIICLLATTPGASLAAYAANGAVHTTTATT
                     TGAPAPFEPARRSDEPARAGAGEVEEDKKPEDKKASGSPSPVAPDETGATPPAQRGTP
                     SQQTTIIIVDERILTGTQSFPQQRSGGRIFLPVASIARALGDVIEVDAAKRSVEVRRQ
                     TGVVARFDAQLNQVSENNALVLSVSNAADITFPPNPDELMLPVEIVSALLDASVRVDE
                     AAHAIRITRGRPQANTVRAGAGRAAFELYQLGYDYNLNSYSAASHQSLTLRADGRIGD
                     GRFNLLTNSSVGGGGGLGLLRNGSFTYERPNEQRFTGGDFGTGTDLLFISSTMRGVSA
                     QLPVRNARLTIFAGRAISGVPTLQTQDSTLSELQTPASIQPHKIRYDTNVFGASATFN
                     LSAGASSSYSPGQTLFSVGMIRFDGAGRSGQMLTASARTASARGRFQGDVAVGRFSGT
                     RPDGARVEGGALAADLSAAYDLSNRLTIQGRYTQIGANFLSAQAGMYEPVRAASGGAT
                     WRPADWLAASFTGSFSTRPADALSGQRQRERFLTATFNLTPRRNWPSIFFSHTESSTT
                     KAGGSYTLVNAAKDFSGWRLFLNATRIKSFGAAFLNAQVGANVRLGEAGALGLSQSFG
                     SRGALGGAVDWNGQSFLKERVSFGAGFNYNRIANSPVTTTGRVFTNIQLPRRSTLGFT
                     YSHGQSGTQILFSLRGSLFSTRRADAAFNAPVAELNSYGAFYGRVYQDINLNGHFDAG
                     LDKPQANVKIRVDGSRYVISDESGRYRIDNVHTGEHEITLDLLSVRADLTLLDGAQQA
                     ASLSQGRDSIVDFRLVRTGRITGLVWLDTNGNHRPDDGEQPLAGVRVLTGSNRDTLTD
                     ENGVYVIGDLPPGEHAVLIDEKTLPEKTVSASGTRAAKVLAGSESGNINFPITPAPPE
                     IKRFASNGN"
BASE COUNT          858 a         1485 c         1361 g          789 t
ORIGIN      
        1 gaagtgctgt catggatcgg ggcgggcgag ttgaaggtga gaatcgagaa gcgatttccg
       61 ctcggtgagg cagcggaagc gcacaggcag cttgaagggc gcgcgaccac gggcaaagtc
      121 ctgttgctgc cttgagcgtc cggcggcgac ggacaaattt caggcgcggc tcgtgagttg
      181 agtgtgagtt gagcgtgagt tgagcgtgag ttgagcgcgg gctatgtcgc gtcgcatccg
      241 aaggctctct cgcgtcgcag gccatcacag gccggtgcgg tgtgtggtcg gcgcgttaag
      301 gcttacgccc atcgttcatc aagacggaag caaggctgcg gggcgaggct gaaaggatca
      361 gttcgatgct gccctgcggc tgccagcgca cctgcgtttc cacttccaac tcgattaaca
      421 cttccagagc attgaagggc gaatcggctg tgccggagag agagatcaac tgcccctgca
      481 aaagatttgc cggggaagaa agacccttca tgctccgatg cgcgtttcgc ccctgaacgc
      541 ccgccgtccc gtcctcgcac gtcgccgtcc tgacggcgtt gaccgccccg cccgcgacgt
      601 gtcttccggt cgccctcaca ctcctgacac atagcccgcg cagcagcgtc gccccgctca
      661 agtttgtcgc agcggagagc gtgtagccga cgttggaacg aagttgtaag ggaatgttga
      721 tgcgcctgtc gctgccgatg ccggaggcgt ctatcgagag gcgtacggtc tgcctgtcga
      781 gattgctgta agtgagcggc agcgtctcgc cggagagttg cgcgccggga gctatcgaga
      841 cgaagaccgc ttcgctgacc tgcccgttcg cggcgaccga agcggcggcg gtttgccctc
      901 tcgctgccgg agcgagaagg atcgtcaaag ccaacgcgat tgagagcgga gcaaaagagc
      961 gcataaccaa acgacatccg tcggagcaag atacgggtgc gccggtagac cacagggcac
     1021 ggcgcaacgc gtccggcctc cggagtctca acggcagatc catcaactcg ttttgcctga
     1081 tagaaagaac gaatcgcgcg cagtttctat aagaaaaacc gcccggcgca agcctctctc
     1141 gcaaaggctc cgggggcgcg ttgcggcgcg gcggctgaca aaacggcagg cggctgacaa
     1201 aacgtcggac atgcctgtca atcgcccctc ccgccccgct tcgcgcagca cagacagcgc
     1261 gtataaccct tatatttcct tcctcacaga acaattaaat tcgctcccgc gcggcacgcg
     1321 ggttgccctg atacagcccc ggcgaactct ttgtgcggag cgtcgcatcc ttcgcccgat
     1381 gccggatgcg acgccctctt taactccctt cacgaaatct ctcaactccg gtgcttcaaa
     1441 tcgcaccgct ccgagcgcaa aacatttcac ggctgccgaa acagacaatg gacaattcaa
     1501 gctcaatcag aactgccatt gccgccgtgt gcatcatctg cctgttggcg acgacgcccg
     1561 gcgcgagcct cgccgcctat gccgcgaacg gcgcggttca cacgacgacg gcgacgacaa
     1621 cgggcgcgcc tgctcccttc gaaccggcgc gacgcagcga cgagcctgca cgtgcgggcg
     1681 cgggcgaagt tgaagaggat aaaaagccgg aggataaaaa ggcgtcgggc agtccgtctc
     1741 ccgtcgcacc ggacgaaacg ggcgcgacgc ctccggcaca acgcggaact ccttcacaac
     1801 agaccacgat catcatcgtg gacgaaagga tactgacggg gacgcagagt ttcccgcagc
     1861 agcgcagcgg cggtcgtatc tttctccccg tggcgagcat cgcacgcgcg ctcggcgacg
     1921 tgattgaggt tgatgcggcg aaacgcagcg tcgaagtgcg tcgtcaaacc ggggtcgtcg
     1981 cgcgcttcga cgcgcagttg aatcaagtca gcgaaaacaa tgcgttggtg ctctccgtct
     2041 ccaacgcggc ggacatcacc tttccgccca accccgatga attgatgctt cccgtggaga
     2101 tcgtctccgc gctgctcgat gcctccgtgc gcgtggacga ggcggcgcac gccatacgca
     2161 tcacgcgcgg gcggccacag gcgaatacgg tgcgggcggg agcgggacgc gcggctttcg
     2221 agctttacca gttgggctat gattacaacc tcaacagcta ctccgccgct tcgcaccaga
     2281 gtttgaccct gcgcgccgac gggcgcatcg gcgacgggcg gttcaacctg ctgacgaatt
     2341 ccagcgtcgg gggcggcggc gggcttggcc tgctgcgcaa cggctctttc acctacgagc
     2401 gtcccaacga acagcggttc accggcggcg atttcggcac gggcaccgac ctgcttttca
     2461 tctcttcgac catgcgcggc gtgtcggcgc agcttcccgt acgcaacgcg cggctgacga
     2521 tattcgccgg gcgtgccatc tccggcgttc cgaccttgca gacgcaagac tccacgctct
     2581 cggaacttca aacgcccgcc tcgattcagc cgcacaaaat tcgctacgac acgaacgtct
     2641 tcggcgcgtc tgcaactttc aacctgtcgg ctggcgcgtc gtcctcgtat agtcccggcc
     2701 agactctctt ctccgtcggc atgattcgct tcgacggcgc aggcaggagc ggccagatgc
     2761 tcaccgccag cgcgcgcacg gcctccgcgc gtggacgctt tcaaggcgac gtggccgtcg
     2821 gacgtttcag cggcacgcgc ccggatggcg ctcgcgtcga aggcggcgcg ctcgccgcag
     2881 acctctccgc cgcctacgac ttgagcaacc gcctcaccat tcagggacgc tacacgcaga
     2941 tcggcgcaaa ttttctgagc gcacaggcgg gcatgtatga accggtgcgg gcggcctccg
     3001 gcggcgcaac gtggcgtccc gccgactggc tcgccgcttc cttcacgggc agcttctcga
     3061 cgcgccccgc cgacgcgctc tccggacaac gccaacgcga acgcttcctc accgccacct
     3121 tcaacctcac gccgcgacgc aactggccgt ccatcttttt ctcccacaca gagagcagca
     3181 cgacgaaggc gggcggctca tacacgctcg tcaacgcggc gaaagacttt tcggggtggc
     3241 gtctgtttct gaacgcgacg cggattaaat ctttcggcgc ggcttttctc aacgcgcagg
     3301 tcggcgcgaa cgtgcgcctc ggcgaagcgg gcgcgttggg gctgagccag tcgttcggca
     3361 gtcgcggcgc gctcggcggc gcggtagatt ggaacgggca gtccttcctt aaagagcgcg
     3421 tcagcttcgg cgcgggtttc aactataacc gcatcgccaa ctcgcccgtc acaaccaccg
     3481 ggcgcgtctt caccaacatt cagttgccgc gccggagcac gctcggcttc acctattcgc
     3541 acgggcagag cgggacacag atcctcttca gcctgcgcgg ctcgctcttc agcacgcgcc
     3601 gtgcggacgc cgcctttaac gcgcccgtgg ccgaactcaa ctcttatggc gcgttctacg
     3661 gtcgcgtcta tcaggatata aacctcaacg ggcacttcga cgccggactc gataagccgc
     3721 aggcgaacgt caagattcgc gtggacggca gccgctacgt gatctctgac gagagcgggc
     3781 gttacagaat tgataacgtc cacacgggcg agcacgagat cacactcgac ctcttatcgg
     3841 tgcgcgccga cctgacgctc ctcgacgggg cgcagcaagc ggccagcctc tcgcaggggc
     3901 gcgattcaat cgtcgatttc cgcctcgtcc gcaccgggcg catcaccggc ctcgtctggc
     3961 tcgacacgaa cggcaaccat cgtccggacg acggcgagca accgctcgcg ggcgtgcgcg
     4021 tcctgacggg cagcaaccgc gacacgctga cggacgaaaa cggcgtctac gtgatcggcg
     4081 acctcccgcc cggcgaacat gcggttctga ttgacgaaaa gacgctgccg gaaaagaccg
     4141 tctcagcctc cggcacgcgc gcggccaaag tgctcgccgg aagcgaaagc ggcaacatca
     4201 acttccccat cacacccgcc ccgcctgaga tcaaacggtt cgcctcgaac ggaaactaag
     4261 cggcaagttc atcaactaca cgactacacg aaagcccacg aatcaaagct gagtttgatt
     4321 cgtgggcttt cgttcgcgtc gtggcctccg gcgcgcttct tttagaagtc taactcgtgc
     4381 tatcgagcgg gcacggtcgc cgccggtttg acggccacgg cgttatcttt gctctctttg
     4441 acgatcaggt ctgtgacgcc ttcggtcacc ttgccgccgt cttgaaaatc cac
//
LOCUS       DASWNS010000408         5051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_483960, whole genome shotgun sequence.
ACCESSION   DASWNS010000408 DASWNS010000000
VERSION     DASWNS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483960"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VGB76_14205"
     CDS             <1..896
                     /locus_tag="VGB76_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100098.1"
                     /translation="YDVQMRARIILANRDGTFAKELVPHGSFENVSHTMAYYNGVMAS
                     RGRAYAGHTWIKDETVERILHDAFRKTDDLFSAFFSEQIGKHEPELTERLVATMEGEF
                     EPVKGVVEVWGKALVSGRTKVEFSHRDVQPQEDEWHADIAFVLNCSLEAEFAKKHAIL
                     VQSKKMNHNAETGLFSQSWTIDIEQCRELVERTAFAYYFLYGPHEFPSVKTLVVPATT
                     LLGIVDATAAPGQGSKNVRYEDVRHSARSLADFMLYDFIGCWVGDPGAEVISLAEGGG
                     GVRPVARHLVTVTIIKGRELQ"
     gene            1040..>5051
                     /locus_tag="VGB76_14210"
     CDS             1040..>5051
                     /locus_tag="VGB76_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100099.1"
                     /translation="MGIREDSIREAIRNMDGNAFERFAYRVLYEERYKGLNPTSETYD
                     FGEDARTEWTALFQHQGKWVSVSASKTGTLAKLKEDCQTAKDKGRRIDVIVFATAGEP
                     RRDKEEEWKKEIKSEFGWDLEVWSLEYFARIASADRHASLVDDYLQIPPPGGDFLHNI
                     ETEFARLTSQVLNSAVVGIGRLDEPLARAEVSHIEEQLRAGKHVLLTGEAGTGKSGIS
                     VLLARASQARGLCVLLVDARRLGYVESEDDFRKYFSLNGPVEDAIIRVARKRGCLVII
                     DQFDNAVRAPSARFLTRLALACYQIENAEIVIISRKVEYYEEKSLKELKRPNVVELTS
                     HQLTEVRAAGVLEELGIQNPPRDLVVVCQNLLNLSLVATIKDERPDYDFSATLNEVDL
                     WEAFVEALAREEGAGVRPDFGDLVVEEAVRLAGEAVRSGDRSVVLARTHPTEQRRLDS
                     WQVIVREEERIYRFRHQRMQDFLVAKGAVSVGTLPGELLKEFDKHRLRGVFVWMDKLY
                     ARRPDSSRRAKFLKELFTLSGVTFDALAEVLQRYINSGDPAADAVALSIILETLRADK
                     GVRDYFFGSRPHPGWVPVLWNEGFFNESPSPQPFGDNHILPPWVEQHYLISVAADLPD
                     LLTEHLKRLRGDSGYVEQAVRALCLLKGEQAEPAVPRLRQWLSDYGVGLTIAGAVVDV
                     IKWFVAEGRTVAALKLFGGLMAPHPAPRPQEIGGHVYAGEAVALFSSSGGFAHKELPE
                     LVRLLAEVAPKEVGNVLENHLLEAIRLEAQTRKFSHQYVWGSGNDPIAESKGFGRDEY
                     KHILLRSLRQTLSEWAGRDAEAVRPIIEGYLTQNRSPLEGGSVLRRLAVYLLQKYAAH
                     YQDLVRQELLDPESLNTRDPKDEFLMLMQKGHQSLTGEEREALIERISRGLPPAIKAQ
                     FGGQNEQGGTTEQDQYFLHYEKKWIRDHLAVLRACLSESQIRLLSELIEEVGEPISPG
                     APQTPYRPSQVRESTITREGLAAMSPDELVEFARSWRPEADEDGMAVMSYKLLADLIA
                     EEVLINPVKYASAVNKLALIRHQFAAALIIRLTNTDRYPLSWEGRISLCESLLADDTV
                     RTDVSARAYDGWTLVRLDMVRLIARWVDKRLLPAHKEYLPPEYLVRVRDILLTFCNDP
                     DPELDETVQEGKLSEKEVPLIAHGHVRPSALSTLILYAVYKAKLEPSLADDDSVSLGG
                     CSRLEPAVREILTRKLDREADPSWAVHSVYGEHLCRLHWLDSRWVDEQLERIFPASDD
                     KRLRWYFVAAWDSFISQNEWEWFFGRNGEVRLNLFERMRGNYSRAIENLSTGWATRSS
                     PQPARDLAGHLLIEYLH"
BASE COUNT         1112 a         1365 c         1560 g         1014 t
ORIGIN      
        1 actacgacgt gcagatgagg gcgaggataa tcctggcgaa ccgcgacggc acctttgcca
       61 aggagttagt gccacacggc agcttcgaga acgtaagcca tacaatggcc tattacaacg
      121 gggtgatggc ttcgcgaggg agggcctacg ccgggcacac ttggattaag gacgagacag
      181 tcgagcgcat cctccacgac gccttcagaa agacggacga cctcttttcc gcgttcttca
      241 gcgagcagat aggcaagcac gagccggagc tgacggagag gctcgtcgcc acaatggagg
      301 gggaattcga gccggtgaag ggcgtggtcg aggtttgggg gaaggcgcta gtatcgggcc
      361 gcacaaaggt cgagttctcg caccgggacg tgcagccgca ggaggacgag tggcatgccg
      421 acatcgcctt cgtgctcaac tgctcgctgg aggccgaatt cgccaagaag cacgcgatcc
      481 ttgttcagag caaaaagatg aatcacaacg cggagacggg tctcttctca caatcgtgga
      541 cgattgacat cgagcagtgc cgcgaattgg ttgagaggac ggcgttcgcg tactactttc
      601 tttacgggcc gcacgaattc ccgtcggtga aaacactggt cgtacccgcg acgactctgc
      661 tcggcatagt agacgccact gcggccccgg ggcaggggag taagaacgtc cgctacgagg
      721 acgtgcgcca ctccgcccgc tcgctcgccg acttcatgct ctacgacttc atcgggtgct
      781 gggtcggcga ccccggcgcg gaggtcattt cgctggcgga aggagggggc ggggtgcgtc
      841 cggtcgcacg gcacttggtc actgtaacaa taatcaaggg tcgggaatta caataaccta
      901 cgggagtcac tcggacatac tcaaccttcc gtagccggga tatacccttt aagtctccca
      961 ttctgtgagc caggcaccag ccgttaatgt ccgcgtgcgg ccaaccacgc attcccaatg
     1021 ggtcttccgt tcaaatgaga tgggcataag agaagacagc attagagagg caatccgaaa
     1081 tatggacggc aatgcgttcg agcgctttgc ctaccgcgta ctctacgaag agcgctacaa
     1141 agggctcaac ccgacttccg agacctacga tttcggcgag gacgctcgga cggagtggac
     1201 ggctctcttt caacatcagg gtaaatgggt ctccgtcagc gcctcgaaga ccgggacgct
     1261 cgccaaactg aaagaggact gccagacggc aaaggataaa gggcggcgca tcgacgttat
     1321 cgtctttgcg acagcgggtg agccccgacg ggataaggag gaggagtgga aaaaggagat
     1381 aaagagtgag ttcggctggg acctcgaagt ctggtcactt gaatacttcg cgcggatagc
     1441 ttccgccgac aggcacgcca gcctcgtcga cgattacctc caaatccccc cgcccggcgg
     1501 cgacttctta cacaacatcg aaaccgagtt cgctcgcctt acttcacaag tcctcaattc
     1561 cgccgttgtg ggtattggga ggttggacga accgctcgcc cgtgccgaag tatcccacat
     1621 cgaagaacag ttgcgcgcag gtaagcatgt gctgctgacg ggcgaagcgg gaaccgggaa
     1681 gagcggcatc agcgtactgc tcgcccgcgc atcgcaggca agagggctgt gtgtactcct
     1741 agttgatgca aggcggctcg gctacgttga gagcgaggac gatttccgta agtatttcag
     1801 ccttaacggc cccgtcgaag atgccatcat ccgcgtcgcc cgtaaacggg gctgtctcgt
     1861 catcatcgac cagttcgaca acgccgtccg ggccccctcc gcccggtttt taacaagact
     1921 tgcgttggcc tgttaccaga ttgagaatgc ggaaatagta atcatctcta gaaaggtcga
     1981 atattacgaa gagaagtcac ttaaagaatt gaagcgccct aacgtcgtcg agctgacaag
     2041 ccaccaactc acggaagtta gggctgctgg tgtactggaa gagcttggta tacagaaccc
     2101 cccgcgtgac ctcgtagtgg tctgccagaa tctgcttaat ctctcgttag tcgcgacgat
     2161 aaaagacgag cggcccgatt acgacttctc tgccaccctg aacgaggtcg atctgtggga
     2221 ggctttcgtc gaggcgttag cgcgggaaga aggagccggg gtgcggcccg acttcggaga
     2281 cctcgtagtc gaggaggcgg tgaggttagc cggcgaggcg gtgcgaagtg gcgaccgcag
     2341 tgttgtttta gcgcgcaccc atccgaccga gcagcggcga ctcgatagtt ggcaggtgat
     2401 tgttcgtgag gaggagcgca tttaccgctt ccgtcaccaa cggatgcagg actttctagt
     2461 ggcgaagggg gccgtgagtg ttggcacgct ccccggcgaa ctccttaaag aatttgataa
     2521 acatcgcctc cgcggagtct tcgtgtggat ggacaagctt tacgcgcgca ggcccgactc
     2581 atcgcgcagg gcgaaattcc tgaaagagct ttttacatta agcggggtga cgtttgacgc
     2641 cttggcggag gtgcttcagc gctacatcaa ttccggcgac ccggcggcgg acgcggtggc
     2701 cctgtcgatc attctcgaaa ctctacgagc cgacaaaggg gtgcgtgatt atttcttcgg
     2761 cagcagaccc caccccggct gggtgcctgt tttgtggaat gaggggttct tcaatgaatc
     2821 tccgtctccg cagccttttg gagataacca catcttacca ccttgggtcg agcaacatta
     2881 tctcatctcg gtggcagccg acctcccgga cttgttaacc gaacacctca aacgtctgcg
     2941 cggggacagc ggttacgttg agcaggcggt cagagcgttg tgtctactca aaggcgagca
     3001 ggctgaaccc gcggtgccga ggctgcgcca atggctgagt gattacggtg tcggactaac
     3061 gatagctggc gccgtcgtcg acgtcatcaa gtggtttgtg gcagaggggc gtacggtggc
     3121 ggcgctcaaa ctgttcggcg ggctgatggc acctcaccca gcgccacgcc ctcaggagat
     3181 aggggggcac gtctatgccg gtgaagcggt cgcgcttttc agcagcagcg ggggctttgc
     3241 ccacaaggag cttccggaac tcgtccggct gctcgcggag gtagcgccga aagaggtagg
     3301 gaacgtcctc gaaaatcatc tcctcgaagc gatccgactt gaagcgcaaa cgcggaaatt
     3361 ctctcaccag tacgtgtggg gatctgggaa tgatccgata gccgagtcga aggggttcgg
     3421 ccgtgacgag tacaaacaca tcctgctacg gtcattacgc caaaccctga gtgagtgggc
     3481 tggccgagac gcagaagcgg ttcgaccgat cattgagggt tatcttactc agaaccgctc
     3541 ccccttggaa ggcggctccg tcctacgccg tctggccgta tacctgctgc aaaaatacgc
     3601 cgcgcactat caagacttag tccgccaaga gttgcttgat cccgagagcc ttaacacgcg
     3661 cgacccaaaa gatgagtttt tgatgctgat gcagaaaggt caccagtctc ttacgggtga
     3721 ggaacgggag gcgttgatcg agcgaatttc acgcgggctg ccgccggcaa ttaaggcgca
     3781 gttcggaggt cagaacgagc agggcggcac gaccgaacaa gatcagtatt tcctgcatta
     3841 cgagaagaag tggattcgtg atcacttggc tgtgttgcga gcctgcctgt ccgagagtca
     3901 aatccggcta ctctctgagt tgattgaaga agtcggagag ccgatctccc cgggagcccc
     3961 gcaaacgcct taccgcccgt cgcaggttag agagagcacc atcacgaggg agggcttggc
     4021 agccatgtcg ccggatgaac tcgtcgagtt cgcccgaagt tggcggcctg aggcggacga
     4081 ggacgggatg gcggtaatgt catacaagct gctggcagac ttgattgccg aagaggttct
     4141 catcaatcct gtgaagtatg cttcggcggt taataagttg gcgctgattc ggcaccagtt
     4201 cgcggcggct ttgatcatcc gcctgacgaa cacagaccgc tacccacttt cgtgggaggg
     4261 gagaatatcg ctttgcgaga gtttgttggc tgacgatacc gttcgcacgg atgtgagcgc
     4321 acgggcgtac gacggttgga ctttggtgcg tttagacatg gtacgtctca tcgctaggtg
     4381 ggtggataaa aggttactcc cggctcacaa agagtacctg ccgccggaat acctcgttcg
     4441 cgtccgcgac attcttctga cgttttgtaa cgaccctgac cccgaactgg acgagacggt
     4501 gcaggagggc aagcttagtg aaaaggaggt cccacttatc gcccatggtc acgtgcgacc
     4561 gagcgcgctt tcgactctca tcctctacgc ggtgtacaaa gcaaagcttg aaccaagtct
     4621 cgcagatgac gactcagtta gcttgggagg gtgctctcgc ttggagccgg cagtgcgaga
     4681 gattttaacg cgcaaactgg acagagaagc tgacccgagt tgggcggtgc attccgtcta
     4741 cggagagcat ttatgcaggc tccattggct cgacagccgg tgggtagacg agcagcttga
     4801 gcgaatcttc ccggcctcgg atgacaagcg actgcggtgg tatttcgtgg ccgcgtggga
     4861 ttccttcatc agtcagaatg agtgggaatg gtttttcggt cggaatggtg aggtcagatt
     4921 aaacctcttc gagaggatgc gaggaaatta ctcccgcgcg attgagaacc tcagtaccgg
     4981 gtgggcgact aggagttcgc cacagccggc gcgggacctc gccggccatc tcctcatcga
     5041 atacctgcat g
//
LOCUS       DASWNS010000409         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_431313, whole genome shotgun sequence.
ACCESSION   DASWNS010000409 DASWNS010000000
VERSION     DASWNS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431313"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1436
                     /locus_tag="VGB76_14215"
     CDS             240..1436
                     /locus_tag="VGB76_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0100100.1"
                     /translation="MTHPAATTPHDYVSQKASRFTESVIREMTREAIKHGAVNLSQGF
                     PDFPAPAAIKEAAMRAIEQDVNQYAITWGARDFREAIAEKTRWYLGLDIDPETEITVT
                     CGSTEGMIAAMMATVDPGEEVIIFEPFYENYAPDAILSDARPRYVPLRAPDWTFDRDE
                     LRATFNERTKAIIICNPNNPTGKVFTRDELEFIAALCREHDALVFTDEIYEHILYPRA
                     GQEIEHISMAQLEGMRERTVIVNSMSKTYSVTGWRVGYCIAPPSITSAIRKVHDFLTV
                     GAAAPLQAAGAMALRLPPAYYEHLQSDYIARRDLLLPILEEAGFKIFRPDGAYYVMTD
                     ISAFNFANDIEFTRHLIQNVGVACVPGSSFYSDPARGAQQVRFCFCKKDETLHAAAER
                     LRKLRA"
     gene            1451..1783
                     /locus_tag="VGB76_14220"
     CDS             1451..1783
                     /locus_tag="VGB76_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100101.1"
                     /translation="MRLTTAEAHSFAAENRRESNLLLCVNSALRAASLCFEGFPSAEL
                     FNSTVWDTKRQIIWLATALIFGTFVVYQHARDEAEGFDPAYFALLEIILLLVIGVMFY
                     FYSKSSDK"
     gene            complement(1867..2769)
                     /locus_tag="VGB76_14225"
     CDS             complement(1867..2769)
                     /locus_tag="VGB76_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100102.1"
                     /translation="MSYRKFFIAASLTLAACLAAAGQAGRRPTASRERATLLNVVAFR
                     EDGSRAPITSKDLAIYDDGIEQSIQNLTPDPGPARIVLLFDNSFSLRTDIPKLANVAR
                     EFAYEIYEGDQLLIAGFDERAEQLSADWTDKTKDVEEAVALLRKKGDPHLFDAVNDVI
                     EVALRPLSGSNRKRVIVLIGDGLDRGSNIKFKDLLAELQKSDITVYALQIPDRTGGAH
                     RRDRPKPTQVVQQLVEGTGGRILPLDKPGESAKIICDELRKNRYLLSYAPTALPYSEM
                     RRLLIIGNPGISVRYKSAMPPNNK"
     gene            complement(2798..3175)
                     /locus_tag="VGB76_14230"
     CDS             complement(2798..3175)
                     /locus_tag="VGB76_14230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0100103.1"
                     /translation="MPIYEYSCQKCRAHVEVMQKITDKPLKRCKSCGGKLEKEWSTSS
                     FQLKGSGWYVTDYAAKKPGVGEKEPAAKSDGATPTDGVAETKTATAPAEAKAEPAAPA
                     VAEETKRKKTSRPAATSNGSKSD"
     gene            3674..4303
                     /locus_tag="VGB76_14235"
     CDS             3674..4303
                     /locus_tag="VGB76_14235"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0100104.1"
                     /translation="MQITRHVPHFRSFKLSLLTLFALFLFVNFSSIGTRVFAPSVAAK
                     IRLDENFIPTDIPTSGDAEIDKMIFSASKEYGVDPRLVHAVAYQESRYKTDARSHAGA
                     QGLMQMMPATATRFAVEGKTGVEQNVYAGTKLLRWLLKRFDGNVSLALAGYNAGEGSV
                     DKYDGIPPYKETENYVRKIVANYGKTYHPVLDPAEANLFFHLTEETAQN"
BASE COUNT          914 a         1362 c         1206 g          983 t
ORIGIN      
        1 aaagcctcgc gttatcgccg ccaattagaa cagccctaac ttgttcgcca gcacgatcag
       61 cagaaacgcg accaccaaaa acgtcgccag ccacgggcgg ttgcccatcg ggcatttgtt
      121 atcccggctc tcttcattca tcgcgcgctc cgagtttgcc tgaaggacgc caactgtgcg
      181 agcatcgcgc cttcagtcga tctatttccg agtcccattt tcgcacgagt tcaatttaca
      241 tgacacaccc cgccgccaca acgccgcacg attacgtctc gcaaaaggcg agccgcttca
      301 cagagtctgt catccgcgag atgacgcgcg aggccatcaa gcatggcgcg gtgaacctct
      361 cacaagggtt tcccgatttt cccgcgcccg ccgcgatcaa agaggctgcg atgcgcgcca
      421 tcgagcagga cgtgaaccag tatgcgatca cctggggggc acgtgatttt cgcgaagcga
      481 tagcggagaa gacgcgctgg tatctcggac tcgacatcga cccggagacg gagatcaccg
      541 ttacctgcgg ttcgaccgaa ggcatgatcg cggcgatgat ggcgacggtt gatccgggcg
      601 aagaggtcat catcttcgag ccgttctatg aaaactacgc gcccgacgcg atcctctccg
      661 acgcccgccc gcgctacgtc cctttacgcg cgcccgactg gacgttcgac cgcgacgaac
      721 tgcgcgccac tttcaacgaa cgcaccaaag ccatcatcat ctgcaacccg aacaacccga
      781 cgggcaaagt cttcacgcgc gacgaactcg aattcatcgc cgcgctctgc cgcgaacacg
      841 acgcgctcgt cttcacggac gaaatctacg agcacatcct ctacccgcgc gccggtcagg
      901 agatcgagca tatctcgatg gcgcaactcg aaggcatgcg cgagcgcacc gtcatcgtca
      961 actcgatgtc gaagacctat tcggtcacgg gctggcgcgt cggttattgc atcgccccgc
     1021 cctccatcac ctcggccata cgcaaagtgc acgacttcct caccgtcggc gcagccgcgc
     1081 cgctccaggc cgccggagcg atggcgttgc ggttgccgcc cgcgtattac gagcacttgc
     1141 agagcgacta catcgcgcgc cgcgatctgc ttttgcccat cctcgaagag gccgggttca
     1201 aaatctttcg ccccgacggc gcgtactacg tgatgaccga catcagcgca ttcaacttcg
     1261 ccaacgacat cgagttcacg cgccacttga tccagaacgt cggcgtcgcc tgcgtccccg
     1321 gctccagttt ctacagcgac ccggcgcgcg gcgcgcagca ggtgcgcttc tgcttctgca
     1381 aaaaagatga gacgctccac gccgccgccg agcgtctacg caaacttcgc gcttaacatt
     1441 ccagtgaatc ttgaggctta caaccgcaga ggcgcacagc ttcgccgcag agaatcgcag
     1501 agagtcgaat cttttactct gcgtcaactc cgcgctccgc gctgcgtcgc tctgctttga
     1561 aggttttcct tctgccgaac tgttcaactc gaccgtgtgg gacacaaaac gtcaaatcat
     1621 ctggctcgca accgcgctca tcttcggcac gttcgtcgtc tatcaacacg cacgcgatga
     1681 ggccgaaggt ttcgatccgg cctactttgc ccttctcgaa atcatcctgc tcctcgtcat
     1741 cggcgtgatg ttctatttct actcaaaaag cagtgacaag tgacgagata agacagtgac
     1801 gggtgacagg cgacaagtta aagacaaggc ttttgtttta actcgtcacc tgtcacccgt
     1861 cacttgtcac ttgttgttag gcggcatggc ggacttgtag cggacgctga tgccgggatt
     1921 gccgatgatg agcaggcggc gcatctctga ataagggagc gcggtcggcg cgtaagagag
     1981 caggtagcga ttcttgcgta gttcgtcgca gatgatcttg gccgattcgc cgggtttgtc
     2041 gagcggcagg atgcgcccgc ccgtcccttc gacgagttgt tgcacgacct gtgtgggctt
     2101 tggccggtcg cggcggtgcg cgccgcccgt gcggtcgggg atttgcaggg cgtagacggt
     2161 gatgtcgctc ttttgcagtt cggcgagcag gtctttgaac ttgatgttgc tgccacggtc
     2221 gaggccgtcg ccaatgagca cgatgacgcg cttgcgattc gagccggaga gcgggcgcaa
     2281 ggccacttcg atcacatcgt tgacggcgtc gaacaggtgc gggtcgccct tctttctaag
     2341 gagcgcgacc gcctcttcca cgtctttcgt cttgtccgtc cagtcggcgg aaagttgctc
     2401 tgcgcgttcg tcgaagcccg cgatgagcag ttggtcgccc tcataaattt cgtaggcaaa
     2461 ctcgcgcgcc acgttcgcca gtttcggaat gtccgtgcgc agggagaaag agttgtcgaa
     2521 gaggaggacg atgcgcgcgg ggccggggtc gggcgttaag ttctggatgc tctgttcgat
     2581 gccgtcgtcg tagatggcga ggtctttgga ggtgatgggc gcgcgtgaac cgtcttcgcg
     2641 gaaggcgacg acgttgagca gggtggcgcg ctcgcgcgag gcagtgggac ggcgtccggc
     2701 ctgtcctgcc gccgctaggc aagccgccaa cgtaagactg gcggcgataa aaaatttacg
     2761 atacgacact ggcttggctc cgggtgcggg ttggaattca atcgcttttc gagccgttgg
     2821 atgtcgcggc aggccgagag gttttcttgc gtttggtttc ttctgcgacg gcgggagcgg
     2881 cgggttcggc tttggcttcc gccggagcgg tggccgtctt agtctcagcc accccgtccg
     2941 tgggggttgc gccgtcgctc ttcgcggcag gctctttttc gcccacgccc ggttttttcg
     3001 cggcgtagtc cgtcacgtac caaccggaac ctttgagttg aaaactactc gtagaccatt
     3061 ccttctccaa cttcccgccg caactcttgc aacgcttcaa aggcttatcc gtgatcttct
     3121 gcatcacttc gacgtgcgca cggcacttct ggcaactgta ctcgtaaatc ggcataaatc
     3181 tttccgtcgt ctctaaggaa gtaaaattga gtttgaactt ttactctgca cgcgattata
     3241 cgaagcgcgc cagcgtgggc gcaaccgcgt atcgaattta gcgcggagag ccgccgtcgc
     3301 agccattttt attagataag cgcaagaaaa gtttcctcaa aacagaaatt tttgccttct
     3361 ttcagtacgc taaaacctct tccgacctcc gaattcatcg ctcaggatga acgactttgg
     3421 agagaagctt tgccctgtca gtgacttgcg cgcgagtgga ttaaatacag acagcaaaag
     3481 gacttatttg cataagtttc gtgcgctgga agcaaccgga ggaggagttc gtccatctta
     3541 tccccggttt cgataatgct ctttgcacat cgtttccacc atgcgtagcc cccaaccttt
     3601 gcaccgcatt taacgatgca accgatggcg tcaaacttcg gtttgaccca agctcacatg
     3661 gagaacgaac tgaatgcaga ttacccgaca cgtccctcat ttccgctctt tcaaattatc
     3721 gctcttaacc ttattcgctc tctttctctt cgttaacttt tccagcatcg gcacgagagt
     3781 ttttgctcct tcggtagcgg cgaaaattcg cctcgacgaa aacttcatcc ccacggacat
     3841 ccccacgtcc ggcgacgccg aaatagacaa gatgatcttc agcgcgagca aagagtacgg
     3901 agttgacccg cgcctcgtcc acgccgtcgc ttatcaggaa tcgcgctaca agaccgacgc
     3961 cagatcgcac gccggtgcgc agggcttgat gcagatgatg cccgccaccg ccacacgttt
     4021 cgccgtcgaa ggcaagacgg gcgtggaaca gaacgtctat gccggaacga aacttctgcg
     4081 ctggctcttg aagcgtttcg acggcaacgt ctcgctcgcg ctcgcaggct acaacgccgg
     4141 tgaaggctcg gttgacaagt acgacggtat cccgccctac aaagagactg aaaactacgt
     4201 ccgcaagatc gtcgccaact acggcaaaac ttaccacccc gttctcgacc ccgcagaggc
     4261 aaacctcttc tttcatctca ccgaagaaac cgcgcagaat taaacgtcga agtacaaacg
     4321 atgaaggatg aatgaaagcc tttctgcttt tcattcatcc ttcatcgttc ctacttcatc
     4381 cttctctttg ctatcatctg ttcttaacaa tcctctcgct tgaagcgcgc acggtgtctt
     4441 gccggttgaa tttcgcccgc accct
//
LOCUS       DASWNS010000410         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_249310, whole genome shotgun sequence.
ACCESSION   DASWNS010000410 DASWNS010000000
VERSION     DASWNS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249310"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VGB76_14240"
     CDS             complement(<1..750)
                     /locus_tag="VGB76_14240"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEY0100105.1"
                     /translation="MRHLPSLLLVLTLIYTPALAQQPPAPLAAEQKPVPLPPPRVKID
                     AYRERAGRIIGAALTSDRAYRRLAHLTDHIGHRLSGSNNLERAIQWALAEMRADKLDN
                     VRAEKVMVPHWVRGAESLELTSPVTRSLTMLGLGNSVGTPPAGITAEAVVVRSFDELD
                     ALGERVRGKIVVYNVPFTTYGATVQYRGAGASRAARHGAVAALVRSVTPVSLQTPHTG
                     AMNYAPDQPKIPAAAITIEGAELLQRMNDRGE"
     gene            836..1099
                     /locus_tag="VGB76_14245"
     CDS             836..1099
                     /locus_tag="VGB76_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100106.1"
                     /translation="MRTPRQPAPATLDPRSIQTAAQLPLNLFVLRIVSSYLAGDASYK
                     FFRRTTPLDEAATLSCSGGHARASDLRPAVLGGVRRGGCGSDF"
     gene            1083..2324
                     /locus_tag="VGB76_14250"
     CDS             1083..2324
                     /locus_tag="VGB76_14250"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0100107.1"
                     /translation="MAQTSNGGLQPLSRADAHVSKADVWPQWGGARRDFKSDAKGLAT
                     RWPAGGPRKLWERALGEGHSSIVSDGARLYTMYRRGEREYVVSLDAASGRTLWEHEYA
                     APILPKMDMTYGFGPHSTPLLTGELVCSVGTTGKLLCLNRASGKKVWGHDLWTDFNGS
                     RINVGYASSPIAFREKIIVQVGGAGHSLMAFNRRDGSVAWQAQNFRNSSSSPIIIDLE
                     GEEQLVAFMHNEVVGLKPESGDLLWRHAVSAGWDFHFNISTPVWGEGNLLFVSAAYGI
                     GGRVLQLSRAGGKTSVRELWQSERTRVHKENAIRLGDVLYASTGHLGPAFFTAMDVKT
                     GRVLWQDRGFSHASFLYADGKFIILDEDGTLGLASPTPTGLNVHSRVELLSGTSWTVP
                     TLANSVLYIRDRKNILALRLN"
     gene            complement(2437..3873)
                     /locus_tag="VGB76_14255"
     CDS             complement(2437..3873)
                     /locus_tag="VGB76_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0100108.1"
                     /translation="MAENNQHLIAHLLRAVEELVVPLRASIGMIWQGEKNSAPDARMV
                     LENQLLLTAQHFIHAREATTADKQNFYEEICRRFRLRPSNDAFAASRPDYRQTPPAMN
                     FDRTPVSVSYLETYDARHATSYADKTRHLLFQFANLVIKFDGTITPAEERALSEFKEI
                     LYPAGRSGAAAAPVNQARAANAEHEPQLTEPEDAPRPLEDLLAELDALVGLERVKADV
                     RQLINFLKVQKMREEQGLRGLTTSRHLVFYGNPGTGKTTIARLLAQIYRTLGLLRRGH
                     LVETDRGGMVAGFVGQTALKVKEVVERALGGVLFIDEAYALTPAGGASDFGQEAVETL
                     LKLMEDHRDDLIVIVAGYTERMQEFLDSNPGLRSRFSKHLHFDDYDPAQLTRIFQTFC
                     RKADFALSPAADAELAKIFSLLSNCRDETFGNARLARNLFETTVNKQANRIVSLPAID
                     KTTLAAIEAADIPNAEDLRASGMLLQTT"
     gene            4096..>4752
                     /locus_tag="VGB76_14260"
     CDS             4096..>4752
                     /locus_tag="VGB76_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100109.1"
                     /translation="MKIFSGSVLILAACVCVAAQVGARREAGGGGLPAGYWSADKSGA
                     IIAKTQTVRLSPDLSHLSEGERRAVAKLLEVGQIFQDVYERQRHADALASRRALAALD
                     RRTNSIPATQNLLALYRLNNGPIATTLDNKREPFLPVELTPPGANVYPWGITKPEVES
                     FLAAHPERKDAILDLRTVVRRATAQNLRADLAVLRQYPVLSTLHPGLQSELTKMQARP
                     D"
BASE COUNT          853 a         1444 c         1546 g          909 t
ORIGIN      
        1 ctcgccgcga tcattcatgc gctgcaaaag ctccgcgcct tcgatggtga tggcggcggc
       61 gggaatcttc ggttgatcgg gcgcgtagtt catcgcaccc gtgtgcggcg tctgtagact
      121 gacgggcgtg accgagcgca cgagcgcggc gaccgctccg tggcgcgccg cgcgcgacgc
      181 acccgccccg cgatattgca cggtcgcgcc gtaagtggtg aacggcacgt tatagacgac
      241 gatcttgccg cgcacacgct cgccgagcgc gtcgagttcg tcgaaactgc gcacgacgac
      301 cgcttccgcc gtgatgcctg cgggcggcgt gcccacgctg ttgccgagtc cgagcattgt
      361 cagggaacgc gtgacgggac tcgtcaactc aagactctcc gcgccccgca cccaatgcgg
      421 aaccataacc ttttccgcgc gcacgttatc cagtttgtcg gcgcgcatct ccgcgagcgc
      481 ccactggatg gcgcgctcta aattgtttga accgctcaag cgatggccga tgtggtcggt
      541 caaatgggcg agcctgcgat aggcgcggtc gctcgtcagc gcagcgccga tgatgcgccc
      601 ggcgcgttca cggtaggcgt cgatcttgac gcgcggcggt ggcagcggca cgggcttctg
      661 ttcggcggcg agcggcgcgg gcggttgctg cgcgagagcg ggcgtgtaga tcagcgtcag
      721 gacgaggagg agcgatggta aatggcgcac gttagagttg gcctccggaa gcggctttaa
      781 gagagcatct ttgacagacg ttgagacgcc tccattttag ccgagtttat aaacaatgcg
      841 cacgccccgc caacctgctc cggcaacgct cgacccgcgt tcgattcaga cagccgcaca
      901 actcccgctc aatctctttg tcctacgaat cgtttcgtcg tatcttgctg gcgatgcgag
      961 ctataaattt tttcgacgca caacgccgct cgatgaagcc gcgacgctct catgcagcgg
     1021 cgggcacgcg cgcgcttctg atctccggcc tgctgtgttg ggcggcgtgc ggcgcggcgg
     1081 ttgtggctca gacttctaac ggcggcctcc aacccttgag ccgcgcagat gcgcacgtct
     1141 ctaaggcgga cgtgtggccg cagtggggcg gcgcgcgtag agatttcaag tccgacgcca
     1201 aaggactcgc cacgcgctgg cctgcgggtg gaccccggaa gttgtgggaa cgcgcgctcg
     1261 gcgaaggcca ttcgtcaatc gtgtccgacg gtgcgcggct ctacacgatg tatcgcagag
     1321 gcgaacgcga gtacgtcgtc tcgctcgatg ccgcgagcgg gcgaacgctg tgggagcacg
     1381 aatacgccgc gccgattctg ccgaagatgg atatgactta cggcttcgga cctcactcca
     1441 ctccgctgtt gacaggcgaa ctcgtctgct ccgtgggcac gaccggaaaa ctgctctgcc
     1501 tgaatcgagc gtcgggcaag aaagtctggg gacacgatct ctggactgac ttcaacggct
     1561 cgcgcatcaa cgtcggctac gccagcagcc ccatcgcttt cagagagaag atcatcgtgc
     1621 aggtcggcgg cgcgggtcac agcctgatgg ccttcaaccg gcgcgatggc agcgtcgcgt
     1681 ggcaggcgca gaacttccgc aactcttctt cgtcgcccat catcatcgac ttggagggcg
     1741 aagagcagtt ggtcgccttc atgcacaacg aggtggtcgg cctcaagcct gagagcggcg
     1801 acctgttgtg gcggcacgcg gtcagcgcgg ggtgggattt tcacttcaac atcagcacgc
     1861 ccgtgtgggg cgaaggcaac ctgctcttcg tctctgcggc ttacggaata ggcgggcgcg
     1921 tgctgcaact gagccgcgca ggcggcaaga cctctgtgcg cgaactctgg caaagcgagc
     1981 ggacgcgcgt gcacaaggag aacgcgattc gcctcggcga cgtgctctac gcttccacag
     2041 gacacctggg tcccgccttc ttcacggcga tggatgtaaa gactggccgc gtgctctggc
     2101 aggaccgagg cttctcgcac gccagctttc tctatgccga cggcaagttc atcattctgg
     2161 acgaagacgg gacgctcggc ctcgccagcc ccacgcccac gggtcttaac gtccattcgc
     2221 gcgtggaact gctgagcggc acttcctgga cagtgcccac gcttgccaac tccgtgctct
     2281 acatacgcga ccgcaaaaac atcctggcgt tgcggctgaa ttagcgccgg gcgtgtttcc
     2341 cgcgctgcga agtaaaagaa gagaggtgct catgctccgg tctgtccttc gcctcaggcc
     2401 gaccgtagcg cagaacgatt aaagcggaac cgctcgtcag gtagtttgca ggagcatgcc
     2461 ggaggcgcgc aggtcttcgg cgttggggat gtctgccgct tcgatggcgg ccagcgtggt
     2521 tttatcaatc gcgggcagag agacgatgcg gttggcctgt ttgttgacgg tcgtttcgaa
     2581 gagattgcgc gcgaggcgtg cgttgccgaa agtctcgtcg cggcagtttg agagcagcga
     2641 gaagatcttc gcgagttccg cgtcggctgc gggcgagagg gcgaagtcgg ctttgcggca
     2701 gaaggtctgg aagatgcgcg tcagttgcgc cgggtcgtaa tcgtcgaagt gcaggtgctt
     2761 gctgaaacgc gagcgcagcc ccggattcga atcgagaaac tcctgcatcc gctccgtgta
     2821 accggcgacg atgacgatca gatcgtcgcg gtgatcttcc atcaacttca aaagcgtctc
     2881 gacggcctcc tgtccgaagt cgctcgcgcc gcccgccggg gtgagcgcgt aagcctcgtc
     2941 aataaagagc acgccgccga gcgcgcgttc caccacctct ttcactttca gcgcggtttg
     3001 accgacgaag cctgcgacca tcccgccgcg atccgtctcg accagatgcc cgcgacgcag
     3061 taggccgagc gtccgataga tctgcgcgag caggcgcgcg atagtggttt tgcccgtgcc
     3121 ggggttgccg taaaagacca ggtggcgcga agtggtcagc ccgcgcaagc cctgctcttc
     3181 gcgcatcttc tgtaccttga ggaaattgat gagttggcgc acgtccgcct tcacgcgctc
     3241 aagcccgacg agcgcgtcca attccgcgag caggtcttcg agcgggcgcg gggcatcttc
     3301 cggctcagtc aattgcggct cgtgctctgc gttagccgcc cgcgcctgat tgacgggcgc
     3361 tgccgccgcg ccgcttctgc ccgccgggta gagtatctct ttgaactcgg agagggcgcg
     3421 ctcctctgcg ggcgtgatcg tgccgtcgaa cttgatgacg agattggcaa actggaaaag
     3481 gaggtggcgc gtcttgtccg cgtaactcgt ggcgtggcgc gcgtcgtaag tctcaagata
     3541 agagacgctg acgggcgtgc ggtcgaagtt catggccggg ggcgtctggc ggtagtccgg
     3601 tctggacgcg gcgaaagcgt cgtttgaggg gcgcaggcgg aagcgacggc aaatctcttc
     3661 gtagaagttc tgtttgtcgg cggtcgtcgc ttcgcgcgcg tggatgaagt gctgagccgt
     3721 gagcagtaat tgattctcaa gcaccatccg cgcgtccggc gcggagtttt tctctccctg
     3781 ccagatcatc ccgatgctcg ccctgagcgg gaccaccagt tcctctacgg ctctcagcag
     3841 gtgtgcgatc agatgttggt tattctcggc caatgaagtt ttccgccgcc gctttgtgtg
     3901 tcgttgcctg agccaacgaa ttcgtcgaga caggattata catgcgcgcg tgcgccgggg
     3961 cgcaccggat acgcggcgcg gcgtagagag gagacgggcg cggctgcttc tttaaggcag
     4021 ttgcttccgg taaacttggc ggcagacaaa atcagcaacg gataaaatcg gtggcatcag
     4081 tagcaagggt aaaacatgaa aatcttttcg ggatcagttc tcatattggc cgcgtgcgtg
     4141 tgcgttgcgg cgcaggtggg cgcgcggcgc gaggcgggcg gcggaggatt gcccgccggt
     4201 tattggtcgg cggataagag cggggcgatc attgccaaga cgcagaccgt gcgcctgtcg
     4261 ccagacctct cgcacctgag cgagggcgaa cgccgggcag tcgctaaact gctcgaagtc
     4321 gggcagattt ttcaggacgt gtacgagcgg caacgccacg ccgacgcgct cgcttcaaga
     4381 cgcgcgctcg ccgcgcttga ccgccgcacc aactccattc cggccacgca aaatctgctc
     4441 gcgctctatc gcctcaataa tggacctatt gcgacgacgc tcgacaacaa acgcgagccg
     4501 tttctgcccg tggagttgac gccgccgggg gcgaacgtct acccgtgggg catcacgaag
     4561 ccggaggtgg aaagttttct cgccgcgcac ccggaaagaa aagatgccat cctcgacctg
     4621 cgcaccgtcg tccgccgcgc gacggcgcaa aatttacgcg ccgacctcgc cgtgctcagg
     4681 cagtatccgg tgctctcgac tctgcatccg ggattgcaaa gtgagttgac gaaaatgcag
     4741 gcgcgccccg at
//
LOCUS       DASWNS010000411         4256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_325659, whole genome shotgun sequence.
ACCESSION   DASWNS010000411 DASWNS010000000
VERSION     DASWNS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325659"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VGB76_14265"
     CDS             complement(<1..698)
                     /locus_tag="VGB76_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100110.1"
                     /translation="MRLRQFSICAILAASVFSISLAGSFQQAGGQRAGAAAATKDLLI
                     FTVTRRADASFEFEPVANFRRGAFEGSITGESSEAELTRFANSYYKAGQRYRLIWGGA
                     EAGTVVAKESQKASDCAKAAATADVQTSVQLGGNRMALATNSPALGLPKSSRRPPTEP
                     ERAGVREHAERIFREKGVPAAALASLQTINLTATDLNSDGAAELIGTYMIKRGTSAIF
                     IDQLFLIAEPAGNA"
     gene            complement(870..2459)
                     /gene="pruA"
                     /locus_tag="VGB76_14270"
     CDS             complement(870..2459)
                     /gene="pruA"
                     /locus_tag="VGB76_14270"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEY0100111.1"
                     /translation="MQGEFRNEAFTDFSKEENARAMRGAIERVRGDLGREYPLVIGGE
                     RIKTGRTFDSINPANKTEVVGRFQKATEELARRAVETADETFKTWRNTLPEERARLLF
                     TVAEILRERRHEMSAWMIFEVGKTWAEADGDTAEAIDFCEFYAREMLRYAAPQPIVKL
                     EGEENRLEYIPLGVGVVIPPWNFPLAIMAGMTCASFVTGNTVVLKPSSDAPTIAYKFF
                     EILEEAGVPAGVVNFMTGSGGEVGDVVVDHPRTRYVAFTGSKEIGLRINERAAKHQEG
                     QVWIKRVVAEMGGKDAIIVDSDTDLDEAATGVVQSAFGFQGQKCSACSRAIIHADVYE
                     PMLEKIAERTERLKLGDPTDAATNLSAVINQKAFKTISEYIEKGQSDGGRVIAGGGSD
                     GEQGFYIEPTVIADVKPGDRVEQEEIFGPVLAVIKAQDFDDALRIANDTEYGLTGAVY
                     TNDAEKLERAKREFHVGNLYLNRKCTGALVGVHPFGGFNMSGTDSKAGGRDYLGLFMQ
                     AKVWAEKVRPGENAKEKRTAI"
     gene            complement(2602..3114)
                     /gene="def"
                     /locus_tag="VGB76_14275"
     CDS             complement(2602..3114)
                     /gene="def"
                     /locus_tag="VGB76_14275"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0100112.1"
                     /translation="MALLEIVTYPNRVLETPGEPVTVFDEKLKKLVADMFETMDAASG
                     VGLAAPQVGVSKRLFVMDCSGGRDPEQRVALINPVVLSVEGDQTGDEGCLSFPGVFFP
                     VKRSLRAVVRAQDVSGETFELDGMDLTARCMLHETDHCDGIVFIDHTTVLKREMVKRK
                     MRRLAKMAKM"
     gene            3653..>4256
                     /locus_tag="VGB76_14280"
     CDS             3653..>4256
                     /locus_tag="VGB76_14280"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEY0100113.1"
                     /translation="MRLRIGVSIAGDKLNAWGAGEERMGETSSAVEEVKEAAEEVAEK
                     VGKHPWTERLARIGYAAKGVVYIVVGALATLTASGFGGKAQGTQGAMRSIARQPFGTA
                     MLSIVAFGLVAYVIWRWVQAVTDADDKGTKAKGIALRLGYAGSGFIYAGLALSAARIV
                     FGAREDGRASAAQSWTATVMQLPYGNWLVTLAALGVMGYGV"
BASE COUNT          777 a         1341 c         1275 g          863 t
ORIGIN      
        1 aaggcgttgc ccgcaggctc cgcgatcagg aacaactgat cgatgaaaat cgcagacgtg
       61 ccgcgcttga tcatgtaggt tccgatcaac tccgccgcgc cgtccgagtt gaggtcggta
      121 gccgtcagat tgatcgtctg caacgaggcg agcgcagcgg cgggcacgcc cttctcgcgg
      181 aagattcgtt ccgcgtgttc gcgcacgccc gcgcgctccg gctcggtcgg agggcggcgc
      241 gaagacttcg gcaggccgag cgcgggggag ttggtggcga gcgccatgcg attgccgccg
      301 agttggacgg acgtttgcac gtcggcggtg gcggctgctt tcgcgcagtc gctcgccttc
      361 tgcgactctt tggcgacaac cgtgcccgcc tccgcgccgc cccagatcag acggtagcgc
      421 tgccctgcct tgtaatagct gttggcaaag cgcgtgagtt cagcctcgct cgactcgccc
      481 gtgatcgagc cttcgaacgc gccgcgacgg aagttcgcaa ccggctcaaa ctcgaaggaa
      541 gcgtcggcgc gtcgcgtcac agtgaagatc agaaggtctt tcgtcgccgc tgctgctccg
      601 gcgcgctgcc ctccggcctg ctgaaaacta cctgccagcg agatcgaaaa aaccgacgcc
      661 gcaaggatcg cgcagatgga gaactgtcga agtcgcatag tctctattct tctccggtcg
      721 ggtgaatgtt gttcgagctt gtgtgcgttt acaaactttc gagtcgtctt ccccgtgcgt
      781 ctccgtcgtg agatttaatg cctgaaccgg aattaactca cgacggagac gcgcgggtag
      841 aacgcaaggg cgggttttgc agggcgcgct tagatggccg tgcgtttctc tttcgcgttc
      901 tcgccggggc gcactttctc cgcccacact ttcgcctgca tgaagaggcc gaggtagtcg
      961 cgcccgcccg ctttggagtc cgtgcccgac atgttgaagc cgccgaacgg gtgtacgccg
     1021 acgagcgcgc ccgtacactt gcggttgaga tagaggttgc cgacgtggaa ctcgcgtttc
     1081 gctcgctcca acttctccgc gtcgttcgta tagaccgcgc ccgtcagccc gtactcggtg
     1141 tcgttggcga tcctgagcgc gtcgtcgaag tcctgcgcct tgatgacggc gagcacggga
     1201 ccgaagatct cttcctgctc gacgcggtcg ccgggtttca cgtcggcgat cacggtcggc
     1261 tcgatgtaga agccctgctc gccgtcggag ccgccgcccg cgatgacgcg cccgccatcg
     1321 gactgcccct tctcgatgta ttcgctgatc gtcttgaaag ccttctggtt gatgaccgcg
     1381 ctcagattcg tcgcggcgtc ggtcgggtcg cccagtttca gacgctccgt gcgctcggcg
     1441 atcttttcca gcatcggttc gtacacgtcc gcgtgaatga tggcgcgcga acaggcggag
     1501 cacttctgac cctgaaagcc gaaggccgac tgcaccacgc ccgtcgcggc ttcgtccaaa
     1561 tccgtgtccg agtcaacgat gatcgcgtcc ttgccgccca tctccgcgac gacgcgtttg
     1621 atccagacct gcccctcctg atgtttggcg gcgcgctcgt tgatgcgcag gccgatctcc
     1681 ttcgagccgg tgaaggccac gtagcgcgtg cgcgggtggt caaccaccac gtcgcccact
     1741 tccccgcccg aacccgtcat aaagttgacg actccggctg gcacgcccgc ctcttcgagt
     1801 atctcgaaga acttgtaggc gatggtcggc gcgtcggaag agggcttgag gacgaccgtg
     1861 ttgcccgtga cgaaagaagc gcaggtcatg cccgccatga tcgcgagcgg aaaattccag
     1921 ggcgggatga cgacgcccac gccgagcggg atgtattcga gccggttctc ttcgccttcg
     1981 agtttgacga tgggttgcgg tgcagcgtag cgcagcatct cgcgcgcgta gaactcgcag
     2041 aagtcaatcg cctccgccgt gtcgccgtcg gcctccgccc aggtcttccc gacttcaaag
     2101 atcatccatg cagacatctc gtgtcgccgc tcgcgcaaaa tctccgcaac cgtaaacagc
     2161 aggcgcgcgc gttcttcggg cagggtgttg cgccacgtct tgaaagtctc gtcggcggtt
     2221 tccacggcgc ggcgtgcgag ttcttcggtc gccttctgga aacgcccgac cacttcggtc
     2281 ttgtttgcgg gattgatgct gtcgaaggtg cgccccgtct tgatccgctc gccgccgatg
     2341 acgagcgggt attcgcgccc gaggtcgccg cgcacgcgtt caatcgcgcc gcgcatcgcc
     2401 cgcgcattct cttccttgct gaaatctgta aacgcctcat tacgaaattc gccctgcatg
     2461 aatcgctcct gttcgggaaa aagttgttct gattttggtt gacgcccgga atgttatcac
     2521 aacccggacg cgaaaacgcg gtcggctcgc atccgtgcga accgaccgcg tctcattgct
     2581 tatcatccgg ggcgacgttt actacatctt cgccatcttc gccagacggc gcatcttgcg
     2641 tttgaccatc tcgcgtttga gcacagtcgt gtgatcgatg aagacgatgc cgtcgcagtg
     2701 gtcggtttca tgcagcatgc agcgcgcggt taaatccatg ccgtcgagtt caaacgtctc
     2761 gcccgacacg tcctgcgcac gcacgacggc gcgcaaactt cgcttgacgg gaaaaaagac
     2821 gccgggaaaa gagagacacc cctcgtcgcc cgtctggtcg ccctccaccg acagcaccac
     2881 cggattgatg agcgcgacgc gctgctccgg gtcgcgcccg cccgaacaat ccatcacaaa
     2941 cagacgcttc gacacgccca cctgcggcgc ggccaacccc acgcccgaag ccgcgtccat
     3001 cgtctcgaac atatccgcga ccagtttctt caacttctcg tcaaacaccg tcaccggctc
     3061 gcccggcgtt tccagcaccc gattcgggta agtcacaatc tccaataaag ccatcttgat
     3121 aattcctgta acagactccg gtatgaattc gcggcgcact caagaaagca cccgctcgac
     3181 gagtttacaa taaactgcga agcgcgtacc agcaacgaaa ttcgagagcc atgttagagc
     3241 ttctcccaat catccttgct gatttcggct tgccgtagga tgcgaatgag aagttctttg
     3301 ccaatgtcgt tctggtgtgg gttggggatg cggagagtga gatttctccg actcatgaat
     3361 tgatgtttcc cgccggaata cgagccgtca aagccaagtt gacgcaaata gcgaatcaac
     3421 atccttgcgc gtgattgaac cacagcgcgg cacttcaggc gacttccctg accttcagtt
     3481 caagcccgtc aacaatgggc agcggcagac tcttcgagac gcgcaacaaa acccactcat
     3541 ccaacacctc cgccaactct tcacaacagg cttcgagcgt ctccgcattg gcataaacgc
     3601 cctcgaagtt tggaatgtct ccgtaatagc tcccatcatc ggggagaatt tcgtgcgact
     3661 tcggattggt gttagcatcg cgggcgacaa attgaatgcg tggggagcgg gtgaagaacg
     3721 aatgggcgag acgagttcgg cggtcgagga agttaaagag gcggcggagg aagttgcgga
     3781 gaaggtcggg aagcacccgt ggacggagcg gctcgcgcgc atcgggtatg cggcgaaggg
     3841 cgtggtctat atcgtcgtgg gtgcgctggc gacgctgacc gcttcaggct ttggcggcaa
     3901 ggcgcagggc acacagggcg ctatgcgttc catcgcgcgc cagcccttcg gcacagccat
     3961 gctctctatc gtcgccttcg gccttgtcgc ttacgtcatc tggcgttggg tgcaggccgt
     4021 gacggacgca gacgataagg ggacgaaggc caaaggcatc gcgctcagac tcggctacgc
     4081 cggcagcggc ttcatctacg ctggcctcgc gctctcggcg gcgcgcatcg tcttcggcgc
     4141 gcgtgaggac gggcgagcga gcgcggctca atcctggacg gcgaccgtca tgcagttgcc
     4201 ctacggcaac tggctggtca cactcgccgc tctcggcgtc atgggttacg gcgtct
//
LOCUS       DASWNS010000412         4142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_198533, whole genome shotgun sequence.
ACCESSION   DASWNS010000412 DASWNS010000000
VERSION     DASWNS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198533"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VGB76_14285"
     CDS             complement(<1..1167)
                     /locus_tag="VGB76_14285"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0100114.1"
                     /translation="MKFALPSAAKKFLFPSLTHARHGLTHACVCVALFCAWSIPQPEA
                     FKIVHSRVRVSESEMDAALQRAATIALGAHEGTIIVTDAQTGRVRAAVNPRMAEEAAF
                     PPGSAIKPFTLLAALRARVINQHARLSCRKHYGRHGADFTCAHPVYNPPFNSAQALAH
                     SCNYYFARLAERLPHDAFNTTLAAYHLGAHEAAAAPATRKASIVKTKAKASAAMMPRL
                     PRGQWQVSTALGEGQELLVTPAQLVAAYAALFNGGQLYQTQTAAPENFRSRQIAQLDI
                     TPDEHALLLAGLRGAVKYGTASASDLASLPTHVFGKTGTATEVGSLHTHGWFVGLAVE
                     RASSVGDDGAHAHAQTEQPTPAEAKLAVLVFLKRGQGKESAAIARAIFAEHARLT"
     gene            complement(1348..2247)
                     /locus_tag="VGB76_14290"
     CDS             complement(1348..2247)
                     /locus_tag="VGB76_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100115.1"
                     /translation="MTKNVLASIFAAARDLVRHPRAFALLIALYLALIAACFFFITTR
                     EATIWQLIVTGASALLAPLLFFVVQAAGASYALGERGAAGLLRRTARSFAKVLLVSLP
                     LVLLAVLAVYLLNKLENRVQLTPEERAQAEYSMSMTEPDAASADAPSPESRPKPPVRW
                     KYLSVSALRLLVLGLFLPLVAMHLWLAVARDGLLAAFARFHRHIFRAFSARSVLTYGV
                     GMIVFALIPYFLITKRTPVSNNTLELLLFGARILIAFTLMLFGWVMTMSALAPHLDGT
                     GATNEPALDALPRPQGAPATAAS"
     gene            complement(2272..>4142)
                     /locus_tag="VGB76_14295"
     CDS             complement(2272..>4142)
                     /locus_tag="VGB76_14295"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100116.1"
                     /translation="HNYLNTDKTTQISLSLNGAQLLDPPTQTVTIPKNGEHRVNWRVS
                     AQQTGDLTLLAKALTDTESDAVELQIPIVPRGLKQTTGEAATLTGDGDSERTITLNLP
                     NNADPRARTLRVEAAPSVAATLFGALDYLTGYPYGCTEQTMSQFLPTVIVANTLRDVE
                     QASIRDTNDIGRKVRRGTNRLYSFQHEDGGWGWWKDDPTDPWMTAYVVDGLTMASRAG
                     YQTDDARVERGRGKLSGLLDAGKNDDGKEIGMETRAYMIYALNASGGVNPRYLEELFT
                     NRGRLQPYARALLALTLKARGDGRARQVAGEIERTANGSDFDAHWKSVRRIENYDEDN
                     SIEATALSIKALAQIAPESGVLPRAVRWVVNSRRRGHYWVSTRQTAFVIYGLTDYLKV
                     SNELSPDYALEVYVNNEQVVAEQVTGAEASGAKSYVVTRRNEQVGGTTQVRFVKRGGG
                     VLYATAALEYATNEEQTAPQSTSGLKITREYLRLRVEEGAEGRLKWKTEPLSGQLRSG
                     DNIVSRLTIEGTAAPYLLIEDPIPAGAQQVENVSGLDLNYSENGWSNWYSAREFRDQR
                     TAFFLNFFGGKATFQYALRVQEPGDFRVAPARVELMYQPNVQSNTASGRLTIMDRQ"
BASE COUNT          680 a         1237 c         1431 g          794 t
ORIGIN      
        1 cgtcaggcgc gcatgttccg cgaagatggc gcgcgcaatc gccgcgctct ccttgccctg
       61 cccgcgtttg agaaagacga ggacggcgag tttggcttcg gcgggcgtgg gctgttcggt
      121 ctgcgcgtgt gcgtgcgcgc cgtcgtcgcc gacggacgag gcgcgttcga cggcgaggcc
      181 gacaaaccat ccgtgcgtgt gaaggctgcc gacttcggtg gccgtgcccg tcttaccgaa
      241 gacgtgcgtg ggcagcgagg cgaggtcgga ggcggaagcc gtgccgtatt tgaccgcgcc
      301 gcgcaagcct gcgagcagga gcgcgtgctc gtcgggcgtg atgtcgagtt gtgcgatctg
      361 ccgtgagcgg aagttttcgg gcgcggctgt ttgcgtctgg tagagttggc cgccgttgaa
      421 gagcgcggcg taggccgcga cgagttgcgc aggggtgacg agcagctcct gcccttctcc
      481 gagcgcggtg ctgacttgcc actggccgcg cggaagcctc ggcatcattg ccgccgacgc
      541 tttcgccttt gtcttaacga tgctcgcttt acgcgtcgcg ggcgcagcag ccgcctcgtg
      601 cgcgcctaaa tggtaagcgg cgagcgtcgt gttgaacgcg tcgtggggga gtcgttcggc
      661 cagccgcgcg aagtagtagt tgcaggaatg tgcgagagcc tgcgcggagt tgaagggcgg
      721 gttataaacg gggtgcgcgc aggtgaagtc tgccccgtga cgcccgtaat gcttgcggca
      781 ggagaggcgc gcgtgctggt ttatcacgcg ggcgcggagc gcggcgagca gcgtgaaagg
      841 tttgatcgcg gagccgggcg ggaaggccgc ctcttccgcc atgcgcgggt tgacggcggc
      901 gcgcacgcgt cccgtctgcg cgtccgtgac gatgatcgtg ccttcgtgtg cgccgagcgc
      961 aatcgtggcc gcgcgctgca aggctgcgtc catctcgctc tcgctgacgc gcacgcgcga
     1021 atggacgatc ttaaacgctt ccggctgcgg aattgaccac gcgcaaaaaa gcgcgacgca
     1081 gacgcacgcg tgagtcagcc cgtggcgcgc gtgcgtgaga gaagggaaga gaaacttttt
     1141 tgccgcggag ggtaaagcga acttcattgc atcaaccagc cgacaactac ttgtccagac
     1201 ctgtgcgcct ctgcgcctca agcgtgagtt tattctttag tcggcctcgg actcacgcgg
     1261 gaggcgcaca ggtcaggcgc gagtctttga tagttacttc gacagttact ttgataatta
     1321 ctttgataat tactttgaca gttactttca actcgccgcc gttgccggtg cgccttgcgg
     1381 tcgcggcaac gcgtcgagcg cgggttcgtt cgtcgcgcct gtgccgtcga ggtgcggggc
     1441 gagcgcggac atggtcatca cccagccgaa gagcatgagc gtgaaggcga tgagaatgcg
     1501 cgccccgaag agcaacagct caagcgtgtt gttggagacg ggcgtgcgtt tcgtgatcag
     1561 aaaataaggg atgagcgcga agacgatcat gccgacgccg tacgtcaaca cgcttcgcgc
     1621 ggagaaggcg cggaagatgt ggcggtggaa gcgtgcgaag gccgcgagca gcccgtcgcg
     1681 tgcgacggcg agccagaggt gcatcgccac gagcggcaaa aagaggccga gcacgagcag
     1741 ccgcaacgca gagacagaca ggtacttcca acgtacaggc ggcttcggcc tcgactcagg
     1801 agatggggca tcggcagacg cggcatccgg ctccgtcatg ctcatcgagt actccgcttg
     1861 agcgcgttcc tccggcgtca actgcacgcg gttctcaagc ttgttcaaaa gatagacggc
     1921 cagcacggcg agcagcacga gcggcaggct gaccaagagc accttggcga aacttcgtgc
     1981 cgtgcgccgg agcaaccccg ccgcgccccg ctcgcccaac gcatagcttg cgcccgctgc
     2041 ctgcacgacg aagaagagga gcggcgcgag cagagccgac gcgcccgtca cgatcagttg
     2101 ccagatggtc gcttctcgcg tggtgatgaa gaagaagcag gcggcgatga gcgcgagata
     2161 gagcgcgatg agcagcgcga aggcgcgcgg gtggcgcacg aggtcgcgcg cggcagcgaa
     2221 gatggaagcg agtacgttct tggtcatagc gtcacctgaa gttcaaactg ttcactgtct
     2281 gtccatgatg gtcagccgcc cgctcgccgt gttcgattgg acgttcggct gatacatcaa
     2341 ctcgacgcgc gccggggcga cgcggaagtc gcctggttcc tgcacgcgca gggcgtactg
     2401 gaaagtggct ttgccgccga agaagttgag gaagaaggcc gtgcgctggt cgcgaaactc
     2461 gcgcgcgctg taccagttgc tccagccgtt ctcactatag ttgaggtcga ggccgctcac
     2521 gttttcgacc tgttgcgcgc ccgccgggat cgggtcttcg atgagcaggt aaggcgcagc
     2581 cgtgccctcg atggtgaggc gcgagacgat gttgtcgccc gaacgcagtt ggccggagag
     2641 cggttcggtc ttccacttca ggcgaccttc cgcgccctct tcgacgcgca ggcgcagata
     2701 ctcgcgcgtg atcttcaaac cggaagtgct ctgcggcgcg gtctgttctt cgttcgtcgc
     2761 gtattcgagc gcggcagtcg cgtagagcac gccgccgccg cgtttgacga aacgcacctg
     2821 agtcgtgccg cccacctgtt cgttcctgcg cgtgacgacg taagacttcg cgcccgacgc
     2881 ctccgcgccc gtcacctgtt cggccaccac ctgctcgttg ttgacataaa cttcgagcgc
     2941 gtagtcgggc gagagttcgt tgctcacttt gagatagtcc gtgaggccgt agatgacgaa
     3001 ggccgtctgg cgcgtcgaaa cccagtagtg accgcgcctg cgactgttga cgacccagcg
     3061 cacggctctc ggcagcacgc cgctttcggg cgcgatctgt gccagagctt tgatcgagag
     3121 ggcggtggct tcgatgctgt tgtcctcgtc gtagttctcg atccggcgaa ctgacttcca
     3181 gtgcgcgtcg aagtcgctcc cgttggccgt gcgttcgatc tcgcctgcga cctgacgcgc
     3241 gcgcccgtcg ccgcgcgcct tcaaagtgag cgcgagcagc gcacgcgcgt agggttgcag
     3301 ccgaccgcga ttcgtgaaca actcttcgag gtagcgcggg ttgacgccgc cgctcgcgtt
     3361 cagcgcgtag atcatgtagg cgcgcgtctc catcccgatc tctttgccgt cgtcgttctt
     3421 gcccgcgtcg agcaggccgg acaatttgcc gcgcccacgt tcgacgcgcg cgtcgtccgt
     3481 ttgataaccg gcgcgcgagg ccatcgtcag cccgtcaacg acgtaggccg tcatccaggg
     3541 gtcggtcggg tcgtccttcc accagcccca gccaccgtct tcgtgctgga agctgtagag
     3601 acgattcgtg cccctgcgca ccttcctgcc gatgtcgttc gtgtcgcgga tggaagcctg
     3661 ttcgacatcg cgcagggtgt tggcgacgat cacggtcggg aggaattgcg acatcgtctg
     3721 ttcggtgcag ccgtaaggat agcccgtcag gtaatcgagc gcgccgaaga gcgtggcggc
     3781 gaccgagggg gcggcttcga cgcgcagcgt gcgtgcgcgc gggtctgcgt tgttgggcag
     3841 gttgagcgtg atggtgcgct cgctgtcgcc gtcgcctgtg agcgttgcgg cttcgcccgt
     3901 cgtctgtttg agtccgcgcg ggacgatggg tatttgtaac tcgaccgcgt ccgactccgt
     3961 atcggtcagg gctttggcta agagcgtgag gtcgccggtc tgctgcgcgc tgacgcgcca
     4021 gttgacacgg tgctcgccgt tcttcggaat cgtcacggtc tgcgtcggcg ggtcgagcag
     4081 ttgcgcgccg ttgaggctca gggagatctg cgtcgtcttg tctgtgttca gataattgtg
     4141 cg
//
LOCUS       DASWNS010000413         9487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_484250, whole genome shotgun sequence.
ACCESSION   DASWNS010000413 DASWNS010000000
VERSION     DASWNS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484250"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VGB76_14300"
     CDS             <1..628
                     /locus_tag="VGB76_14300"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0100117.1"
                     /translation="VETSRGAMRADAFVVACGAWSSLLPLARNQLSSAQPAVQPAARR
                     GSDATVARIEPVRGQMLCFAPRTPPLVRHVVYSPRGYLVPRRDGRLLAGTTTEHAGFD
                     KSLTTAGRRAIESYAREIAPGVIELNVSEAWAGLRPRAADDWPVIGAFGADVPNLFYA
                     TGHYRNGILLAPLTGALVARMISERGLVPDMLKPFTPERFRRALAGAL"
     gene            769..2136
                     /locus_tag="VGB76_14305"
     CDS             769..2136
                     /locus_tag="VGB76_14305"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0100118.1"
                     /translation="MRKRFVPASIALAFIAAHAFHAPARAQSTASVASPAPVPAERSR
                     DVAALYAEAEAYARKKYEAFEREGIGYEQKLVEQVEREQRALAARYAAGVAAREPQDT
                     LSAQEVFYLGQLYNVAEDGVRAVETMRRFLTQNPQATREEQQAARYVVALQTAKKGSP
                     VDEAETALADYLKVAAPDAHERRYRLETALATSYRGQKQAERAIAHAHAAFAAANHLH
                     AAKPLDAATRDAVFYRTLSFISDAALDLKRTDEAIAALQELRRLSVSFPSADLYRRAT
                     VLLNRIVPAVAYVSKPDDPLFRVETGARLAPEIRVADWIEQKPTTLADLRGRVVLLDF
                     WALWCTPCHAALPHISEWQEKYKERGLSVVALTEYRNTLNGRPARTPQEVGELRRFKR
                     ASRLPFGFAVADTQETATAYGVTTIPVAVLIDRRGVVRFLNVGANENDIRELHQMIER
                     LLDEK"
     gene            2264..3652
                     /locus_tag="VGB76_14310"
     CDS             2264..3652
                     /locus_tag="VGB76_14310"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0100119.1"
                     /translation="MRKFLVAVCACCFGVVLLTAAGSAFAQTDATPQGSVPQATPATP
                     KATPPAKLQSGDAATLYEDASKYVERKFEEFKQNRVPFSRALATETFQQQRELAAHHA
                     AQLVARGQIAGTDHYYLGMLYDLAGKSPEAVESLRRFLAQQSADAKAGTKDQWQNARL
                     ILGTEAATLSRFEEAESALAEYTRGEPVTPQGLFKLHHALARAYSKNKKIEQSAAHAR
                     IAFKVAKESPVKAGDESDRAQIIGSAAEFLADVLLSLKKEREANETMEELLRLGLSLP
                     SAQIFTSAARWLAENEQEELIEGVLRETPPESSPRAPEINIAEWIDQQPVKLADLRGR
                     VVLLDFWATWCGPCRFTMPKLKTLHERFKKEGLVVVGLTQFFGREGRTVLTQAEELAR
                     LRAYKKDLKLPYAFAVALDSDNDERYGVRAIPTAFLIDRRGRVRYISVGASGADDEEL
                     LSTIKRLLAEQP"
     gene            3751..5007
                     /locus_tag="VGB76_14315"
     CDS             3751..5007
                     /locus_tag="VGB76_14315"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein/redoxin"
                     /protein_id="HEY0100120.1"
                     /translation="MEGTNLSFSLVIAFVGGLASFLSPCVLPLVPGYISLISGVSIDH
                     LKSGGASRPTNARRAVMLNSLAFNAGLSLIFIALGAAAGLIGSAITSNPWIRIIGGLI
                     IIGFGLQLMGVLKIGALYRDTRKFSDEKPRGMLGSFTLGLAFAAGWTPCIGPILGGIL
                     GLAATSGGWQSGLVLSAFYAAGLAVPFLLTGLGINRFLGFYTRFRRHLHKVELVSGVL
                     LICIGLLVATNNVTRLASYASVLPNPETWVNSWAKRNQPAQPDSAATKASNPNATYAA
                     APDTEMRTLEGAPFRISNLRGRVVLVNFWATWCLPCRAEIPDFNRMQKDYEARGFAVV
                     GISSHDTPEMIRDFQSVVRQEYQLLTSGDDAPQEFQTGPGRPATFIIDREGRIRERFI
                     GERDRATFDAVVLPLLDEPQTTASNQ"
     gene            5052..5708
                     /locus_tag="VGB76_14320"
     CDS             5052..5708
                     /locus_tag="VGB76_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0100121.1"
                     /translation="MRKILSLVLFLCALTFAVAPVSSGMSDAHEYAPIQEKKINYKDW
                     TFKRMGTGEQVNLREWTKGKKLVLVVYLAPWCNNWKLEAPVVERLYTKYKAHGFDVIG
                     VSNYGTERDQQVYFKDKNVPYTVVVESDTREARDKTTHYSYRQLTGDARKWGSPYNIF
                     LMPAKLKKDGDVLTEKAWVNNGELIEAEADEFIRKRLGLKKEKDEEKKKSDEKTATQS
                     "
     gene            complement(5866..6024)
                     /locus_tag="VGB76_14325"
     CDS             complement(5866..6024)
                     /locus_tag="VGB76_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100122.1"
                     /translation="MRENHLTHRRREKFSTLRLSLRLPDNTNGNAPVVLRTTDGATSR
                     SDVLIRVN"
     gene            complement(6052..8247)
                     /locus_tag="VGB76_14330"
     CDS             complement(6052..8247)
                     /locus_tag="VGB76_14330"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEY0100123.1"
                     /translation="MPLATLFLLFTLAANTPDIFAAPNHNAAAAPATTSALDEEDAGY
                     EETARVVRVSMLRGDVSLRRAGSKNWEKATLNLPLVEGDRLATGADARLEIQIDARNF
                     VRLGEYATLDIVTLRDEGVALSLPEGTATLRLANFDNDREYFEIDAPKTTLAAEQKGS
                     YRLDVAQNGNVRVTVREGGRARLYSETSGFTLRDGRTAELVYSDGATDGDWELSAARS
                     SDDWDTWVDERERHLASRLRYDRRERYYDNYVWGAEELDAYGDWVNSGEFGYVWRPHV
                     TVINNYHDWAPYRYGHWRWVSPYGWTWVGDEPWGWAPYHYGRWVYVNNYWCWAPRGYA
                     HYGGRSWWRPALVAFVSFGDSYDSNVCWYPLGYHQRDPRSNFYRRAVNRAIEQQRLAN
                     LDRVNPIYQRAVTTMQARQFGRLNVRGQAATKELALRAVTTEPVRGGLRLRPAEADLR
                     TQGEGQTPVVRERKGDALVVRNPNDPSRVLVERKTGAGARKPGLALDEELRRGRIYNN
                     REPRKIERIGDSNNGGGNTLNSGERGNGKNTQDRISDDNGERKTGAVARPARPIRMPS
                     DGKSVDGGRMTTGAPAIKRPVVNNDVDGRERKVRPVERPGDDATIPTPDYRRERPSVR
                     DERAKPIERERAAPEVYRPRPEVREERKTQREERPEPRYERPAPTREERPAPREERSA
                     PREERSAPPPQREERRPDPPQREERRPDPPRSREDSSPLRQKSKPSDND"
     gene            9090..>9487
                     /locus_tag="VGB76_14335"
     CDS             9090..>9487
                     /locus_tag="VGB76_14335"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0100124.1"
                     /translation="MNNRKKFSRGIVSLLLCVLLLSPLTSVRGQQAQPQSGRGVVTGT
                     VTDASGASVAEAQVFLVGVQQAVLSTTQTDAGGRFNFADVAAGTYELRITRRGFDNRR
                     LPARVLTGETTEVAVTLEVNALAEQITVTA"
BASE COUNT         1713 a         2905 c         3083 g         1786 t
ORIGIN      
        1 cgtggaaact tcgcgcggcg cgatgcgtgc ggacgcgttt gtcgtcgcgt gcggcgcgtg
       61 gtcgtcgctg ctcccgctcg cgcgcaatca actctcttcc gcgcaacctg ccgtccagcc
      121 cgccgcccgg cgtggcagcg atgcgacggt cgcgcgcatc gaacctgtgc gcgggcagat
      181 gctctgcttt gcgccgcgca cgccgccgct cgtccgccac gtcgtctata gcccgcgcgg
      241 ctacctcgtt ccgcgccgcg acgggcgatt gctcgcgggc acgacgaccg aacacgcggg
      301 ctttgataaa tcgttgacca ctgcgggacg ccgagccatc gagtcttatg cgcgcgagat
      361 cgcgcccggc gtcatcgagt tgaacgtgtc ggaggcttgg gcgggattgc ggccacgcgc
      421 ggcggacgat tggcctgtca tcggtgcgtt cggcgcagac gtgccgaacc tcttttacgc
      481 taccggccat tatcgcaacg gcatcctgct tgcgccgctg acgggcgcgc tcgtcgcgcg
      541 gatgatctcg gagcgcggcc ttgtgcccga catgctcaaa ccgttcacgc ccgaacgctt
      601 tcgtcgcgcc ctcgcaggcg cgttgtgact aacagagaga tgaaattttc aaacgaagat
      661 atgagactta tgcaaaggcg caagggtcac gccgggaggc gcgaagcagt acgacgattc
      721 ggagtcgcct ctactcttct tcgcgctttt cggttgagct ttgcgcccgt gcgtaaaagg
      781 tttgtacctg cgagtatcgc gctcgccttc attgccgcgc acgcctttca tgccccggct
      841 cgcgcgcaaa gcacggcctc cgtcgcttcg cctgcgcccg taccggcgga acgctcgcgc
      901 gacgtggcgg cgttatacgc agaggcggag gcttatgcgc ggaagaagta tgaggcgttt
      961 gagcgcgaag ggatcggcta cgagcagaag ttggtggaac aggtcgagcg cgagcagcgc
     1021 gcactcgccg cgcgttacgc cgccggagtc gcggcgcgcg agccgcagga cactttgagc
     1081 gcgcaggaag ttttctatct cggtcaactc tacaacgtcg cggaggatgg cgtgcgcgcc
     1141 gtcgagacga tgcgccgttt cctgacgcag aatccgcagg cgacgcgcga ggagcaacag
     1201 gcggcgcgct acgtcgtcgc gctgcaaacg gcgaaaaagg ggtcgccggt ggacgaggcc
     1261 gaaaccgcgc tcgcggatta tttgaaagtc gctgcgcctg acgcgcacga gcgtcgctac
     1321 cgtctggaga ctgcgctggc gacgagctat cgcgggcaga agcaggcgga gcgcgctatc
     1381 gcacacgcgc atgcggcctt tgcggcggcg aaccatctgc acgccgcgaa gcctctggac
     1441 gcggcgacgc gcgatgcggt cttttatcgc acgctctcct ttatcagcga tgccgcgctc
     1501 gacctgaaac gaaccgacga agccatcgcc gccttgcagg aactcagacg cctgagcgtc
     1561 agtttcccgt ccgccgatct ctaccgccgc gcgaccgtgc tgctcaaccg catcgtcccg
     1621 gcggtggctt acgtgtcgaa gccggatgat cctctgttcc gcgtcgagac gggcgcgcgg
     1681 ctcgcgccgg agatcagggt ggcggattgg atcgagcaga agcccacgac gctcgcggac
     1741 ttgagagggc gcgtcgtgct gctggacttc tgggcgttgt ggtgtacgcc gtgccacgcc
     1801 gcgctgccgc acatcagcga gtggcaggag aaatataagg agcgcggctt gagcgtcgtc
     1861 gcgctcacgg agtatcgcaa cactctgaac gggcgaccgg cgcgcacgcc gcaggaggtg
     1921 ggagaactgc gccgcttcaa acgcgcctcg cgcctgccct tcggcttcgc cgtcgccgac
     1981 acgcaggaaa ccgccacggc ttacggcgtg acgacgatcc ccgtcgcggt cttgattgat
     2041 cgtcgcggcg tcgtgcgctt cctcaacgtc ggcgcgaacg aaaacgacat acgtgagttg
     2101 caccagatga tcgagcggct gttggacgag aagtaataag gcagtgacga gtgacgggtg
     2161 acgagaggga attggtgaga gttgagaatt tcggttgtga gttgtgagtt gccgaagttt
     2221 ggctttgaga gtttgagaag tgggattgcc gggtgagggc gcgatgagaa aatttttggt
     2281 tgccgtgtgt gcgtgctgtt ttggcgtcgt gttgttgacg gcggcgggca gtgcgttcgc
     2341 gcagacggac gcgacgccgc agggaagtgt gccgcaggcg acgcccgcca cgcccaaggc
     2401 gacgccgccc gcaaagctac agagcggcga tgcagcgacg ctttacgaag acgcgtcgaa
     2461 atatgtggag cggaagtttg aagagttcaa acagaaccgc gtgccgttta gccgcgcgct
     2521 ggcgacggaa actttccagc agcagcgcga actggccgcg catcacgccg cccaactcgt
     2581 cgcacgcgga cagatcgcgg gaaccgacca ttattatctg ggcatgctct acgacctcgc
     2641 cggaaaatcg ccggaggccg tggagtcgct gcgtcgtttt ctcgcgcagc aatctgcgga
     2701 cgcgaaagcg ggcacgaagg atcagtggca gaacgcgcgt ctcattctcg gcacggaggc
     2761 cgctaccctc tcccgcttcg aagaggccga aagcgcgctc gcggaataca cgcggggcga
     2821 acccgtcacg ccgcagggac tcttcaaact ccatcacgcg ctcgcacgcg cttactcgaa
     2881 gaacaaaaag atcgaacaga gcgcggcgca cgcgcgcatc gcgttcaagg tggcgaagga
     2941 gtcgccggtg aaagcgggcg acgaaagcga ccgcgcgcag attatcggga gcgcggcgga
     3001 gtttctcgca gacgtgttgc tgagtttgaa gaaggagcgc gaggccaacg agacgatgga
     3061 agagttgttg cggctcggac tctctctgcc gtcggcgcaa atcttcacga gcgcggcgcg
     3121 ctggctggcg gagaacgagc aggaggaatt gatcgagggc gtgctgcgcg agacgccgcc
     3181 ggagagcagt ccgcgcgctc ccgagatcaa catcgccgag tggatcgatc aacagcctgt
     3241 gaagctggcg gacttgcgcg ggcgggtggt gctgctggat ttctgggcga cgtggtgtgg
     3301 tccctgccgc ttcacgatgc cgaagttgaa gacgctgcac gagcgtttca aaaaagaggg
     3361 gctggtcgtc gtcggcctca cacagttttt cgggcgggag gggcgcaccg tcctcacgca
     3421 ggcggaagaa ctcgcacgcc tgcgcgccta caagaaggat ttgaaactcc cttatgcttt
     3481 cgccgtcgcg ctcgattccg acaacgacga gcgttacggc gtgcgcgcca tcccgacggc
     3541 ctttctgata gaccggcgcg ggcgcgtccg ctacatctcg gtcggcgcga gcggcgcgga
     3601 cgatgaagaa ctcctgagca ccatcaagcg tctgctcgcc gaacaaccgt agccagcgga
     3661 aggaacatct cgacccggag cttcgtcttt ctcgattcac gatttacgat tcacgattta
     3721 ctttttgttg gtgtaagctg gcggtcagtt atggaaggca ctaatctcag tttttcgctc
     3781 gtcatcgctt ttgtgggcgg cctcgcgtcg ttcttaagcc cgtgtgtgct gccgctcgtg
     3841 ccgggctaca tctcgctcat ctccggcgtc agcatcgacc acctgaaatc gggcggcgca
     3901 tcccgtccga cgaacgcgcg ccgcgccgtg atgttgaact cgctcgcctt caacgcggga
     3961 ctctcgctca tcttcatcgc gctcggcgcg gcagccggac tcatcggctc ggccatcacc
     4021 tccaacccct ggatacggat catcggcggc ctcatcatca tcggcttcgg cttgcagttg
     4081 atgggcgtct tgaagatcgg cgcgctctat cgcgacacgc gcaagttctc cgacgagaaa
     4141 ccgcgcggca tgctcggttc attcacattg ggtctggcgt ttgccgccgg ttggacgccc
     4201 tgcatcggac ccattctcgg cggcatcctc ggcctcgctg cgacgagcgg cggctggcag
     4261 agcggcctcg tgctctcggc cttctacgcc gccggactcg ccgtgccgtt tctgctcacg
     4321 ggtctgggca tcaaccgttt cttagggttc tacacgcgct tccgacgcca cctgcacaaa
     4381 gtcgaactcg tctccggcgt cctgctcatc tgcatcggcc tcctcgtcgc cacgaacaac
     4441 gtgacgcggc ttgccagtta cgcctccgtc ctccccaacc ccgaaacgtg ggtcaacagt
     4501 tgggcgaagc gcaatcaacc ggcgcagcct gattcggcgg cgacgaaagc atcgaacccg
     4561 aacgccacgt acgccgccgc gcccgacacg gagatgcgga cgctcgaagg agcgccgttt
     4621 cgcatctcaa acctgcgcgg gcgcgtggtg ctcgtaaact tctgggcgac ctggtgcctg
     4681 ccctgccgcg cggagatacc agacttcaac cggatgcaga aagattacga ggcgcgcggc
     4741 ttcgccgtcg tcggcatctc ttcgcacgac acgccggaga tgatccgcga cttccagagc
     4801 gtcgtccggc aggaatacca actcctgacg agcggcgacg atgcgccgca ggaatttcag
     4861 acgggacccg gtcgtcccgc caccttcatc atcgaccgcg aagggcgcat ccgcgaacgc
     4921 ttcatcggcg aacgcgaccg cgccaccttc gacgccgtag tcctccccct gctcgatgaa
     4981 ccgcagacta ctgccagcaa tcaatagagc gagcgaccgc gaaactcgcc gaagaggagt
     5041 taccaacagc catgcgaaaa attttgagcc tcgttctttt tctctgcgcg ctcacgtttg
     5101 ccgtcgcgcc cgttagctcc ggcatgagcg acgcgcacga gtatgcgccg attcaggaga
     5161 agaagatcaa ttacaaggat tggaccttca agcggatggg gacgggcgag caggtgaatc
     5221 tgcgcgagtg gacgaaggga aagaaactcg tgctcgtcgt ctatctcgcc ccctggtgca
     5281 acaactggaa actggaagca cccgtcgtcg aacgcctcta cacgaagtat aaagcgcacg
     5341 gcttcgacgt gatcggcgtg agcaattacg ggacggagcg cgaccagcag gtctatttca
     5401 aagacaagaa cgtgccctac accgtcgtcg tcgaatccga cacgcgcgag gcgcgcgaca
     5461 agacgacgca ctacagctat cgccaactca cgggcgacgc gcgcaagtgg ggatcgccct
     5521 acaacatctt cctcatgcct gcgaaactca agaaggacgg cgacgtgctg acggagaagg
     5581 cttgggtcaa caacggcgaa ctcatcgaag ccgaagccga cgagttcata cgcaagcggc
     5641 tcggtttgaa gaaagagaag gacgaagaga agaagaagag cgacgagaag accgcgacgc
     5701 aaagttaagc aggcgagccg ggcgcgaaag cgcagccggg tgaagggaaa gaaccttcac
     5761 caccgccgga gacgcagaga acgcgaaggg ggcacggaga cttgaatgat ctccgtgccc
     5821 ccttcgcctt ctctgcgtct ctgtggcgac ggtggtgagt gctttttagt tgactctgat
     5881 caacacgtcg ctgcggctcg tcgcgccgtc cgttgtgcgc aggacgacgg gcgcatttcc
     5941 gttcgtgttg tcgggcaggc gcaggctgag acggagagtt gagaattttt ctctgcgcct
     6001 gtgcgtgaga tggttctcac gcacaggcgc agaggggcat tagtaaccgc ttcagtcgtt
     6061 gtctgagggc ttcgacttct gccggagcgg gctgctgtcc tcgcgtgagc gcggcgggtc
     6121 gggtcgccgc tcctcgcgtt gcggcgggtc ggggcggcgt tcctcgcgtt gcggcggagg
     6181 cgcggaacgt tcctcgcgcg gcgcggagcg ttcttcgcgt ggcgcggggc gttcttcgcg
     6241 cgtgggagcg gggcgttcat aacgcggctc cggcctctcc tcgcgctgcg tcttgcgctc
     6301 ttcgcgcacc tccggcctcg ggcgatagac ctccggcgca gcacgctcgc gctcgattgg
     6361 cttggcgcgc tcgtcgcgca ccgacggacg ttcgcgccgg tagtcgggcg taggtatcgt
     6421 cgcgtcgtcg cccggacgtt cgacgggtct gaccttgcgt tcgcgaccat cgacatcatt
     6481 gttgacgacc gggcgtttta tcgcgggcgc gcccgtcgtc atcctgccgc cgtcaacgct
     6541 cttgccgtcc gaaggcatgc gtatgggtcg cgccggacgc gccactgcgc ccgtcttgcg
     6601 ttcgccgttg tcgtcggaga tgcggtcttg cgtgttcttg ccgttaccgc gttcgccgct
     6661 gttgagagtg ttgccgccgc cgttattcga gtcgccgatg cgttcgatct tgcgcggctc
     6721 gcgattgttg tagatacgac cgcgtcgcag ttcttcgtcg agcgcgaggc cgggtttgcg
     6781 cgcgcccgcg ccggtcttgc gttcgacgag aacgcgcgaa ggatcgttcg ggttgcgtac
     6841 gacgagcgcg tcgcccttgc gctcgcgcac gacgggagtt tgcccttcgc cctgtgtcct
     6901 gagatcggct tcggcggggc gcaagcgcag cccaccgcgt accggctcgg tcgttacggc
     6961 gcgcagggcg agttccttcg tcgcggcctg cccgcgcacg ttcaacctgc cgaactgtcg
     7021 cgcctgcatc gtcgtcacgg cgcgttgata gatcggattg acgcggtcca gattggcgag
     7081 acgctgttgt tctatcgcgc gattgacggc gcggcggtag aagttagagc gcgggtcgcg
     7141 ctggtgatag ccgagcggat accagcagac gttgctgtcg taagagtcgc caaaactgac
     7201 gaaggccacg agcgcgggac gccaccaact gcggcctccg taatgagcgt agccgcgcgg
     7261 cgcccagcac cagtagttgt tgacatagac ccaacgcccg taatgataag gcgcccagcc
     7321 ccaaggttcg tcgcccaccc acgtccagcc ataaggcgaa acccaacgcc agtggccgta
     7381 gcgatagggc gcccagtcgt gataattgtt gatgaccgtg acgtgcggac gccacacgta
     7441 gccgaactcg ccggaattaa cccaatcgcc gtaggcatcc agttcttccg cgccccagac
     7501 gtagttgtcg tagtagcgtt cgcggcggtc gtagcgcagg cgcgaggcga gatggcgttc
     7561 gcgttcgtca acccatgtat cccagtcgtc ggatgagcgc gcggcggaga gttcccagtc
     7621 gccgtcggtc gcgccatcgc tgtagacgag ttcggcggtg cgcccgtcgc gcagggtgaa
     7681 gccggaggtt tcggaataga gacgcgcgcg cccgccctcg cgcacggtga cgcggacgtt
     7741 gccgttctgc gccacgtcga gacgatacga acccttctgt tcggcggcga gagtcgtctt
     7801 cggggcatcg atctcgaaat actcgcggtc gttgtcgaag ttggcgaggc gcagcgtcgc
     7861 cgtgccttcc ggcaggctca gcgcgacccc ttcatcgcgc agcgtcacga tgtcgagcgt
     7921 ggcgtattcg ccgaggcgca cgaagttgcg cgcgtcgatc tgaatttcga ggcgcgcgtc
     7981 cgcgcccgtg gcgaggcggt cgccttccac gagtgggaga ttcaaagtgg ccttctccca
     8041 gttcttgctg cccgcccggc gcaaactcac gtcgccgcga agcatgctga cgcgcacgac
     8101 ccgcgccgtc tcttcgtaac cggcgtcttc ttcgtcaagc gcgctcgtcg tcgccggagc
     8161 agcggcggcg ttgtggttgg gggcggcaaa aatgtcgggg gtgttggccg cgagtgtgaa
     8221 gaggagaaaa agggttgcaa gtggcattct aagtagcagg gacgttttgc tgtagcgcat
     8281 ggcgctggct cctttttgaa actggacggg acaagagaga gagagttttc ctcaacccga
     8341 gagacgcgca taggaaagag agcgttacac tttcttaatc gagcgcgacc ggagagaaaa
     8401 gctgtgcgag ggcttcaacc ttacggatag cagggcgcga agcaaagcgc gtgcccgcgt
     8461 gcagatgtgt ccggacgccg gagcatatca caacgtttag acgggaaacc gcacgaacgc
     8521 ttgaaattct catccgagat agtgcagccg cagcactatt tggtatgccc gcgtatgact
     8581 gacctgacgc ctgcgtgctc ccggccatta aattttcgca ctcgaaattt tttttacacc
     8641 ttgagaaatt ttttcatccg catttttgca ggatttttag cggggttttg cctttcgcgc
     8701 ccggtaaaac tcaagttttc ctcaagtctg ttttttatag tggcttcagt tcggcgggct
     8761 ggggggctcg tcgaaggttt tgcgagggaa agatgccggt cgcgtcttgc ccgcttcggt
     8821 tgaaccaagg ctcggatcat cggggaagcg ggtaagaggc gaggcatctt aagcgaaagg
     8881 tcgtcgtgaa ttgcacgtca gcaaaggggc atgacggcta cacgcttctt cgcatagttg
     8941 gccgccggac gttgggggac gtccggcggc ctactttttt ctgcgcctgc cacaccgaac
     9001 tatcgccccg cctgccctcg ccttaacttg tgcaactcgt tcgcgctatc atgcgtcggc
     9061 ctcgattctc aaaaagggat atttgtgcga tgaataaccg gaagaagttt tccagaggaa
     9121 ttgtgagtct cctcctgtgc gtcctcctgc tcagccctct gaccagcgtg cgcgggcagc
     9181 aggcacagcc gcagagcggt cgcggcgtgg tgacggggac ggtcacggat gcttccggcg
     9241 cgagcgtggc agaggcgcag gtttttctcg tcggcgtgca gcaggccgtc ctgagcacga
     9301 cgcagacgga cgccgggggg cgtttcaatt tcgcggacgt ggccgccgga acttacgaac
     9361 tgcgcatcac gcgcagaggc ttcgacaatc gccgtctgcc cgcgagagtc ttaacgggcg
     9421 agacgaccga agtggccgtc acgctcgaag tcaacgcgct cgccgagcag atcaccgtca
     9481 cggcgga
//
LOCUS       DASWNS010000414         4825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_326040, whole genome shotgun sequence.
ACCESSION   DASWNS010000414 DASWNS010000000
VERSION     DASWNS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326040"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VGB76_14340"
     CDS             <1..583
                     /locus_tag="VGB76_14340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100125.1"
                     /translation="GVAERQRLATAAQAAVKAARENPSDYEAQMNAARASFETNRLDD
                     VLEFLLSANKLQPGNLEPVIALGNVNYDTGNYLAAEKWYTAALAKTPDDINVRTDLGL
                     TFLLRTPPDNERALAEFRRSLAIDPKHEPTLQNMVVALTRKGDKMEARATLAKLESVN
                     PENPALPQLRSGLDTGGAAETEGAGKNSAGGKS"
     gene            825..2198
                     /locus_tag="VGB76_14345"
     CDS             825..2198
                     /locus_tag="VGB76_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100126.1"
                     /translation="MRRAGLFRHTIKDFRFITLIIFVSITAMICAYTRAASQSPGAEL
                     AQRRSTSPQTRRRAPSPARPRVDYSRFSHATKGHFENCASCHRITSFAQPDIRDYPDH
                     PSCTECHRQQFFRGARPVICSNCHTVTTPRSEARFKFPKEEEPSQFADIFPHASHIKT
                     TTLLQFKKVVGEKVNIQATCMYCHKVDQTTQKPPAGAPKDAFHAPAGTLMTTPTSHVT
                     CFQCHWQKGVENREQEPLSNECSRCHKNLARPLLQTAALTGALAAPVPQSGAQSRASV
                     AKLIPAVAQGTSVAHKTLTPPRISPKFVHVLNQSDIESDPHRKRTNDEGKRVDITCTQ
                     CHAGVRKAATLEAMQLKVNQVQLPSCSTSACHTALSGTVGLKLSVFRELRERGKDAKF
                     DCALCHAPPVSLNPEVPCSHYKAVLESAKKENEALEKAGKKPRSLTGIEGLTPPRCAE
                     QPKKEGT"
     gene            2205..3206
                     /locus_tag="VGB76_14350"
     CDS             2205..3206
                     /locus_tag="VGB76_14350"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY0100127.1"
                     /translation="MRSKAVEEQTAQRQSNTADAATDAAAARAPRRRARRASVQQTLA
                     FAIVIACVAALVALSVFNHRAAASNASVAPGAPASFEPPPPQGSGVDFSRFLHGSPRH
                     ASLACAACHKRDANNSARPNLPGHKACTDCHLPQFVSNNIPMCAICHTDVQSGNPPVK
                     GFPSIRSFNAKFDHAQHNTGAARPSNGCVACHTPASRRSAALTIPAGFNAHSQCYTCH
                     TSNAQSSGRDIASCGVCHSLAARYFRTPTNARAFRASFSHATHGARQRLGCNDCHNLR
                     AGLAQSRQVTAPAVSQHFNTTRGQSCMSCHNGRRAFGDADFNDCKRCHKAQTFRMGI"
     gene            3440..3997
                     /locus_tag="VGB76_14355"
     CDS             3440..3997
                     /locus_tag="VGB76_14355"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY0100128.1"
                     /translation="MKLFLVLAATLCFAAITLSGNVASLAALSSKVQPKEVILGKDAK
                     ATGQGSKSVLFTHDTHSLKNYSADGKSVIGCAECHHTDQPKSALKGILKTSEREEMLT
                     TASLEKADAKPVKTCRECHAQTDIKPAGWPANPEVTYPDESDPITLTNEEAYHRNCNV
                     CHDAVKKRDPATKAPVTCVQCHNGK"
     gene            4042..4554
                     /locus_tag="VGB76_14360"
     CDS             4042..4554
                     /locus_tag="VGB76_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100129.1"
                     /translation="MRNLVLALTCGAALCAAGCATSPATNNSNGNAATTISNQSSRPS
                     NTGLPPVSSAHGGDSPVAPPATGAAPNSAARPGIDTATLDAKIERAEAKAKGGQQADK
                     IAAAAALVERGNVYRDAGQPALYKFALGDYRRALRYQPDNTEAREKMEEIVGIYQSMG
                     RPVPENGNEP"
BASE COUNT         1063 a         1579 c         1333 g          850 t
ORIGIN      
        1 cggcgtggcc gagcggcagc gtctggcaac tgccgcgcag gcggcggtga aagcggcgcg
       61 cgagaatccc tctgactacg aggcgcagat gaacgccgcg cgcgcttctt ttgaaaccaa
      121 ccgtctcgac gacgtgttag aatttctgct gagcgcaaac aagctgcaac ccggtaatct
      181 ggagccggtc atcgcgctcg gcaacgtcaa ctatgatacg gggaactatc tcgcggcgga
      241 gaagtggtac accgccgcgc tcgccaagac gcctgacgac atcaacgtgc gcaccgatct
      301 cggcctcacc ttcctcctgc gcactcctcc cgataacgaa cgcgcgctgg ctgaattccg
      361 ccgttcgctc gccatagacc cgaagcacga accgacgctg caaaacatgg tcgtcgcgct
      421 cacgcgcaaa ggcgacaaga tggaagcacg cgccacgctc gccaaactcg aaagcgtcaa
      481 tccggagaat cccgcgctgc cgcaattgcg ctccggcctc gatacaggcg gggcagccga
      541 aacagaaggc gcaggcaaga acagcgcggg cggcaagagt tagaatgcgt tccacgaaag
      601 ctacttaatt tcagtgctga cctcgccgtc gcgcttcgtc gccgttcggg agaatatcgt
      661 tgtcagccag tgccaactgt gttacaagga acgtcttctg ctctcgtgct ccaaatttaa
      721 cgggcgacgc atttgctatt gtcacaggat gagcttcggc ggatttttca ggcagtcggc
      781 gtgcgccagt ttcacgaatg accggcagcg aaaggactaa cgggttgcga cgcgcgggtc
      841 tgtttcgtca caccatcaaa gattttcgct tcatcacttt gatcatcttc gtgtccatca
      901 cggcgatgat ctgcgcctac acgcgcgccg cttcgcaaag ccccggcgca gagttggcgc
      961 agcggcggag cacaagcccg cagacgcgtc ggcgcgcgcc ctcgcccgcc cgcccccgcg
     1021 tcgattattc gaggttctcg cacgcgacca aaggccactt tgaaaactgc gcctcctgcc
     1081 atcgcatcac ttcgtttgcg cagcccgaca tacgcgacta cccggaccat ccttcgtgca
     1141 ccgaatgtca tcgtcaacaa ttctttcgcg gcgcgcgtcc ggtcatctgc tcgaactgcc
     1201 acaccgtcac tacgcctcgc agcgaagcgc gcttcaagtt tccgaaggaa gaggagcctt
     1261 cgcagttcgc ggacatcttc ccgcacgcga gccacatcaa gaccactacg ctcttacaat
     1321 tcaaaaaagt cgtcggcgaa aaagtaaata ttcaggcgac ctgtatgtac tgtcataaag
     1381 ttgaccagac gacgcagaag ccaccagcgg gcgcgcccaa agacgccttc catgcgcccg
     1441 ccggaactct tatgaccacg ccgacgagcc acgtcacctg cttccaatgc cactggcaga
     1501 agggcgtgga gaaccgcgaa caggaaccgc tctcgaacga gtgcagccgt tgccataaga
     1561 atctggcgag gccgctcttg caaaccgccg ctctaaccgg cgcgctcgct gcgcctgttc
     1621 cgcagagtgg agcgcaaagt agagcgagcg tggcgaagct cattccggca gtggcgcagg
     1681 gaacttcggt cgcgcataaa actctgacgc cgccgcgcat ctctccgaag ttcgtccacg
     1741 tgctgaacca gagcgacatc gaatcagacc cgcaccggaa gaggacgaac gacgagggca
     1801 agcgcgttga tatcacttgc acgcaatgcc acgccggagt gagaaaggct gcgacgctgg
     1861 aggcgatgca gttgaaggtg aatcaggtgc aactgccttc gtgttcgacc tcggcctgtc
     1921 acacggcgct ctccggcaca gtcgggttga agctctccgt gtttcgtgaa ctgagagagc
     1981 gcggcaaaga tgcgaagttt gactgcgctc tgtgccacgc gccgcccgtg agtttgaacc
     2041 cggaagttcc gtgcagccac tacaaggccg tgcttgagtc tgcgaagaaa gagaacgagg
     2101 ctctggagaa agcagggaag aaaccgagga gcctgacggg catcgaagga ctcaccccgc
     2161 cgcgttgcgc cgaacaaccg aagaaggagg gcacttagac gaacgtgcgc tcgaaggctg
     2221 tcgaagaaca aaccgcgcag cggcagagca acaccgccga cgctgctact gatgctgctg
     2281 ccgcccgcgc gccgcgtcgc cgcgcacggc gtgcgagcgt gcagcaaacg ctcgcgttcg
     2341 caattgtgat cgcgtgtgtg gccgcgctcg tcgctctctc cgttttcaac catcgcgccg
     2401 ccgcttccaa tgcgtccgtc gcgcccggtg cgcccgcgtc attcgagccg ccgccgccac
     2461 agggaagcgg cgtagacttt tccaggttcc tgcacggcag cccgcgccac gcctcgctcg
     2521 cctgcgccgc ctgtcacaaa cgcgatgcga acaactccgc gcgccccaac ctgcccggcc
     2581 ataaagcctg taccgattgc cacctcccgc agtttgtctc gaacaacatc ccgatgtgcg
     2641 ccatctgcca cacggacgtg caatcgggca atccgcccgt caaaggcttt ccctcgatac
     2701 gcagcttcaa cgcgaagttc gaccacgcgc agcacaacac gggcgcggcg cgtccgtcga
     2761 acggctgcgt cgcctgtcac acgcccgcct cgcgtcgcag cgcggcgttg accattcccg
     2821 cgggcttcaa cgcgcattct cagtgttaca cctgtcacac gtcgaacgcg caaagctcag
     2881 ggcgcgacat cgcttcgtgc ggcgtctgcc attcgctcgc cgcgcgctac ttccgcactc
     2941 cgacgaacgc acgcgccttt cgcgccagtt tcagccacgc cacgcatggc gcacgccagc
     3001 gtctcggctg caacgattgc cacaatctcc gcgcaggtct ggcgcagtcg agacaagtta
     3061 ccgcgcccgc cgtctcccaa cacttcaaca cgacgcgcgg ccaaagctgc atgagttgcc
     3121 ataacgggcg acgcgctttc ggcgacgccg atttcaacga ctgcaagcgt tgccacaaag
     3181 cccaaacttt tcgcatgggc atctgacgca cgcgcacgct aaattttcgg gaaagaaaat
     3241 tgacatcgcg cgggggcggt cttatagtga attcgcgtcg gcgagcctgc gaattcgtcg
     3301 ccgcctccaa gttcaaacaa gttcaaagca ctgccaaagt tttgttaacg ctctacacaa
     3361 attttgctat tttcatctcc cctcgtgtct ctaaaaagat tgccgctacg ttagaaccca
     3421 ctttgaagga cgaccagcca tgaaattatt tttagttctt gccgctactc tctgctttgc
     3481 cgcgatcact ctgtcgggta acgtcgcatc gcttgcagcc ctgagcagca aggtgcaacc
     3541 gaaagaagtg attctcggta aagacgctaa agcgacgggg cagggcagca agtctgtact
     3601 cttcacgcac gatacgcact cgctgaagaa ctacagcgcg gatggtaagt ccgtcatcgg
     3661 ttgcgccgaa tgtcaccaca cggatcaacc gaagtctgcg ctcaagggca tactcaaaac
     3721 ttccgaacgc gaagagatgc tgacgaccgc ctctctggag aaggccgacg ccaaacccgt
     3781 caagacttgc cgcgagtgcc acgcgcagac agacatcaag cccgcgggct ggcctgccaa
     3841 cccggaagtc acctatccgg atgaatccga tccaatcaca ctgacgaacg aggaagccta
     3901 tcaccgcaac tgcaacgtct gccacgacgc ggtcaagaag cgcgacccgg cgacgaaagc
     3961 tcccgtcact tgtgtccaat gccacaacgg gaaataagct cacacaggga caatcattca
     4021 agggggggtg gaaacaggaa gatgcgaaat ttagtcttag ctctcacatg tggagccgcc
     4081 ttgtgcgccg ccggttgcgc cacatcgccc gccacaaaca acagcaacgg caacgcggcg
     4141 acgacgatta gtaatcaatc gtcgcgcccg tcgaacaccg gccttccccc agtctcttcc
     4201 gcgcacggcg gcgactcccc ggtcgcgcct cccgcgacag gtgccgcccc gaacagtgcg
     4261 gccaggccgg gcattgatac cgccacgctc gacgccaaga tcgagcgtgc cgaagcgaaa
     4321 gctaaaggtg gacagcaggc ggacaagatc gccgccgccg ccgccctcgt cgagcgcggc
     4381 aacgtctacc gagatgccgg gcaaccggcg ctctataaat ttgccctcgg cgactatcgc
     4441 cgcgccctgc gctaccaacc ggacaacacc gaagcgcgtg agaagatgga agagatcgtc
     4501 ggcatctatc aatcaatggg tcgccccgtt ccggaaaacg gcaacgagcc ataagcctct
     4561 gcctgctacg caaaactaca aaggccgcat gcagcaaatt gcatgcggcc tttgtagttt
     4621 atgtgagcaa ctgtctcgtg cgacgcacga actcagccga tcttgatgcc gatggcttcg
     4681 aggaacttgg tcgggcggtc gccgccaatc atggcgaaga tgtaatcgtt ccggatcgtc
     4741 gttttcactt cctgcgtgcg cgcgttcatc aggacgactt catcgtcgcg tatctcattg
     4801 atggccgtgc cgaaaaagac cttga
//
LOCUS       DASWNS010000415         4345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_379305, whole genome shotgun sequence.
ACCESSION   DASWNS010000415 DASWNS010000000
VERSION     DASWNS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379305"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2634)
                     /locus_tag="VGB76_14365"
     CDS             complement(<1..2634)
                     /locus_tag="VGB76_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0100130.1"
                     /translation="MEIKKASQESKARRRPSRSRYMTTHLDQYFTRLDVYFQKREAEG
                     LGVPESHKTRGVHSANCIATGTGIPAHKLEGVPPLREKIIEWSVKIGFTPAEYRRGGS
                     ARKREYEKKFGDYTARLKREGKKIPVNPGRAGTPDVERISSDSDIPVTCFSRSTGVGR
                     LLRQAIKEVGLEVYPDSHGWGSISYGELLRAGSEWRREELKGAPLADQQRYNTRSSLR
                     YFMLHAGELIRQAGFGEEDMIGVELLEKFEETLRSLTQQIESDSTRDTFSGQMRRWHC
                     YYLRICRTKELPRPFIKALECAMNRAAMGPAQLADAAGVSISKLNAWLNGINTPAVES
                     FPEVRRLEEALSLTADTLTSRVIRTRPKHFPSSAYPEYLTVDGEEVKIRGDHRMRGLL
                     RPLLPDDFDTRAEGERSEMLTELIKNLVRPTTDWGRWHRANTGIITALSKFPSVVEAE
                     FKDLEEFKCSTLPPRGMKRRGSWSKQTVLIRKNSLLGFFGYLSLPLNADDVRLRGLGF
                     DKGLFTLAMLTQPAFIDDWLRWKARKRKGGQDEESYSLFDVDILHDIIGHLELEVGWL
                     RQKPELAMNLKPIPGYIDETFIERARSDWKSVCDEALGEYKSLAESVEEVAEEQRDPF
                     ELILPLLDVDHPSYQNPILALRTFAQNILNDMPDPALAPIKAARHVRSYLTVRILTAT
                     VLRSRNIRELTYRKDNEGHLRKRGDKWIVVIPRKLFKNKHSSFFGSKKKHDYEKVLAD
                     KDGLYGWIEEYINKHRPVLLKGSESDVFFFNTPDYPVTRAPQFHKRYRLLTMYYLAHN
                     PYLNRGVPGVKPHGPHAVRDIFATFVIQLTGSYELAAYILQDSLRTVRDHYTNFMPKD
                     KTRLVDYYLNAAWDGEPRDESP"
     gene            complement(3619..>4345)
                     /locus_tag="VGB76_14370"
     CDS             complement(3619..>4345)
                     /locus_tag="VGB76_14370"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HEY0100131.1"
                     /translation="DLWEASGLPPILRKQRIETALELKRCPDAWVRKHLTIKGLRLVW
                     ELRGIPCLPLEVFEKPRKGLCCSRSFGRPVTTVEDLGEAVAMHCARGGEKLRRQRLAA
                     SHLTVFVSTSRFRQNPEAIYSAVASWRLPFPTSYTPALAGAARALLGRVYKPGFVYQK
                     AGVFLTDIVADTQRQQSLVLRMDDERRLRLMEAVDRINRKHGRHTVRPLAMGNACAWE
                     MRRTQLSGRYTTRLDEVLRVRAC"
BASE COUNT          863 a         1275 c         1188 g         1019 t
ORIGIN      
        1 tggtgactca tctctcggct caccatccca ggcggcgttt aagtaatagt ccacgagcct
       61 ggtcttatct ttcggcatga aattcgtgta gtgatcgcgc acggttctga gtgagtcctg
      121 gagtatgtaa gcagccaact cgtagctgcc cgtgagttgg atgacgaacg tcgcgaaaat
      181 atcccttacc gcatgtggcc catgcggctt cacacccggc acgccccggt tcaggtaggg
      241 attatgcgcg aggtaataca tcgtgagcag ccggtaacgc ttgtggaatt gcggcgcccg
      301 cgtcacgggg taatcaggtg tgttgaaaaa gaagacgtcg gattccgagc ccttaagcag
      361 gacgggtcgg tgcttattga tgtactcctc gatccacccg taaagtccat ccttatcggc
      421 tagcaccttt tcgtagtcat gcttcttctt gcttccgaag aacgagctgt gtttattctt
      481 aaacagtttc cgcggtatta ctacgatcca cttgtcaccc cttttccgga ggtgaccctc
      541 gttatcctta cggtacgtga gttcgcgaat gttacgcgaa cgcaacaccg tcgctgttag
      601 tatcctaacg gtcagataac tgcggacgtg ccgcgccgcc ttgattgggg ctagtgcggg
      661 atccggcatg tcgttgagga tgttctgtgc gaacgtccgc agggccagga tcgggttctg
      721 gtatgatggg tggtcgacgt caaggagggg gaggatgagt tcaaaggggt ctcgttgttc
      781 ctcggccacc tcttccacgg attccgctag actcttgtat tcgcctaatg cctcatcgca
      841 gacgcttttc cagtcacttc tggcgcgctc tatgaaagtc tcgtctatgt agcctggtat
      901 tggcttcaag ttcatggcaa gttccggctt ctgtctgagc cacccgacct cgagttctag
      961 atgcccgatg atgtcatgga gtatgtccac atcaaaaagg gagtagcttt cttcatcctg
     1021 cccacccttt cgtttacgcg ccttccatcg cagccaatca tcgataaagg cgggctgagt
     1081 aagcatcgcc aatgtgaaaa gacccttgtc aaatccgagc ccgcgcaacc gaacgtcatc
     1141 cgcgttcagg ggaaggctga ggtatccgaa gaatccaagc agactattct tacgtatcag
     1201 taccgtctgt ttagaccatg agcctctgcg cttcatcccg cggggaggca gggtcgaaca
     1261 tttgaattct tctaagtctt tgaattctgc ctcgacgacc gacgggaact tactaagcgc
     1321 ggtaataatg cctgtgttag cccggtgcca ccgcccccag tcggtcgtcg gtcgcaccaa
     1381 gttttttatt agctcggtga gcatctccga cctctcaccc tctgcgcgcg tgtcgaaatc
     1441 atccggcaat agcggccgta acagcccccg catcctatga tctccgcgaa tttttacttc
     1501 ctcgccgtcg accgtcaggt actcgggata agcggacgag gggaaatgct tcgggcgcgt
     1561 tctgatgacc ctggaggtca gcgtgtcagc cgtcagcgac agtgcctctt ccagccgccg
     1621 aacttccggg aagctctcga cggccggagt atttatgccg ttgagccacg catttaattt
     1681 ggagatactg acgcctgcgg cgtcggcgag ttgtgccgga cccatcgcgg ctcggttcat
     1741 ggcgcactcc agcgctttaa tgaacggcct gggcaactct ttcgtcctac agatgcgcag
     1801 atagtaacag tgccagcgtc gcatctggcc cgagaaagta tcacgcgtgg agtcactctc
     1861 gatttgttgc gtgagggagc ggagggtctc ctcaaatttc tcgaggagtt cgacccctat
     1921 catgtcttct tccccgaacc cggcctgcct gatgagctcg ccggcgtgta gcatgaagta
     1981 tctaaggctt gatcgcgtat tataacgttg ctgatccgct aagggagcac ctttgagctc
     2041 ttctctacgc cattcactgc ctgctctgag cagctccccg taacttatcg agccccagcc
     2101 atggctatcc gggtagacct ctaatcctac ctctttaata gcctgacgca ggagcctccc
     2161 aacacctgtt gacctcgaaa agcacgtaac aggtatgtct gagtcactgg atattctctc
     2221 cacgtcaggg gttccggcgc gcccggggtt aacaggaatt tttttccctt cccgttttaa
     2281 gcgggctgta tagtcgccga acttcttctc gtattctcgc tttctagccg agccgcctct
     2341 acgatactca gctggtgtga agccgatttt tacgctccac tcaatgattt tttcacgtaa
     2401 aggagggacc ccttctaact tgtgcgcagg aatcccggtg ccggtggcaa tacaatttgc
     2461 gctatgcacg ccacgggttt tatgtgattc aggaacgcct agcccctccg cctcccgttt
     2521 ctgaaaataa acatcaagcc gtgtgaaata ttgatccagg tgggtagtca tatagcgcga
     2581 gcgggacggc cgccgccgag ccttgctttc ctgagatgcc ttctttattt ccatttcgaa
     2641 gtaaggcccg taccccggga ttctaagttg gtagaagtaa actccctcat gcgggctctg
     2701 ttgtggagcg ttatcgcaca ccaaaccggc tggctaaggc gaccggccgt tccgggcaca
     2761 cttatccctc gccctgagag gacgcgtcgt tatcctgagc gagcagactg ttaccggcgg
     2821 aatttaggcc gcactaatcc gtagcgcgcc ttacaagcgc acttcaaaaa tcatggattc
     2881 tacgattgat acgaagggtc tgccgttacg atcgttcgcc cgtgtcctat gccttgaggc
     2941 atcgcgagct aaccgattaa ttagtcatga gccgccttgt gacgcgcgaa atatagccac
     3001 ttgctcaaaa gctgtcaaca gaaaaacctc ttttattata agcatttaac gacgctatgc
     3061 gtcttctaag caggtgggcg ggacacaacg aggatgctgt ggggggtttt taaacccccc
     3121 acagcatggt cggctgtggg tttaaaaacc ccccacgtta gggtcggctg tggggggtgt
     3181 aaaaaccccc cacgttagag tcggctgtgg gtttaaaaac cccccacggg tggatacatc
     3241 gttggggggt ttttacaccc cccacagaga ttaaggcgtt gagcccatca ctgataaata
     3301 acttacggca gtgctgtcac tggcccctgc tcgtgacaag gcccgaagat cgtcgggcct
     3361 aacgactcga acggtcttac ctgtctgccc caacgccttg ttatgcccgc cgccgcgcgg
     3421 ttggaattca ggtcggcctc gctgaccgat caggtgccgg acaaggatag tcgtatagga
     3481 gtttcgacgg acgtcggatg cggccggttc agcttcagca acttcccctg ttaaactaac
     3541 taacccgccg tacgttattc ggattacccg ccgaagccat tgcatgcccg gagtgccgct
     3601 gcttcggagt tgctcaagct agcaagccct gactctcagc acctcgtcga gccgcgtggt
     3661 gtagcggccg gagagctgag ttcgcctcat ctcccacgca caggcattcc ccatcgccag
     3721 cgggcgcacg gtgtgacggc cgtgcttgcg gttgatccgg tctacagcct ccatgagccg
     3781 caggcgccgt tcgtcgtcca tgcgaagcac caaactctgc tgcctttggg tgtcggccac
     3841 tatgtctgtg aggaacaccc ccgccttctg gtagacgaag cccggcttgt agacgcggcc
     3901 gagcagggcc cgcgccgccc cggccagcgc cggcgtgtag gaagtcggga aggggagccg
     3961 ccagctcgcc accgccgagt agatcgcttc ggggttctgg cggaagcggg acgtggagac
     4021 gaacacggtg aggtgtgagg cggccagccg ctgcctccgc agcttctcac ccccgcgcgc
     4081 gcagtgcata gccaccgcct cgcccaggtc ctcgaccgtc gtgacgggtc tgccgaagga
     4141 gcgcgagcaa catagccctt tgcgcggctt ctcgaagacc tcaagcggca ggcagggtat
     4201 gccgcgcaac tcccacacga gccgcagccc tttgattgtg agatgcttcc tcacccacgc
     4261 gtccggacag cgcttcagct ccagcgccgt ctcgattctc tgtttgcgca ggataggcgg
     4321 cagccccgag gcttcccaca ggtcg
//
LOCUS       DASWNS010000416         8215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_198866, whole genome shotgun sequence.
ACCESSION   DASWNS010000416 DASWNS010000000
VERSION     DASWNS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198866"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(394..933)
                     /locus_tag="VGB76_14375"
     CDS             complement(394..933)
                     /locus_tag="VGB76_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100132.1"
                     /translation="MKLKWHGFPCRHSATPLALLLLIVGCVTFIFASNHQNNTTEPAA
                     LKTLFDFTYVGIGEGGFDPSALPHIPKEELSGKTLPDRLAKGHQYVFIRAKRKKGESM
                     FELIQSRLTDNGASIEQASCCILAYAGDPVFMIRFRDKKYKGVIYNALNTAIYFDTTL
                     REQYDGTFYVLVVEEELSE"
     gene            complement(963..5339)
                     /locus_tag="VGB76_14380"
     CDS             complement(963..5339)
                     /locus_tag="VGB76_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEY0100133.1"
                     /translation="MSYDERVYNSSNQMLRRTLTGWSTITPSGSTRPRDPHVTKTVGL
                     ILDTGGTALSSTTMMVYDADLNVTAKHSYDYSSVDQTTAQTGAIGEIPGGTLLRTDET
                     TYLVNDANISAATRDAYRARNMVGLPSSTRVKTAANTVNTVVAQSEVKYDEAAYSLLP
                     SYGAVVGWTDPGTNVRGNVTTTRSWLDTSNSWIETHARYDQVGNPRYAWDAKGNVSEV
                     EYSSAYHYAYPTHTKSSVPDPTNNRATNTPLESWMAYDFSTGKITSTTDANSKTTVLK
                     YTYHDQYGNVVRDALDRLRKVEQPDGGWTAYEYGRNAYGDYVRTRTAINATQTTDGYD
                     YFDGLGRSVRSFRSEGSQSSTTDTQYDAMGRVWRVSNPYLSAGVGSAINPSGRWTTTA
                     YDALGRVLTVTTPDSAVVSTSYSGVQVTVTDQSGKVRRSVTDALGRLTQVVEDPAGLA
                     YQTSYLYDVLGNLRKVDQGGQLRFFMYDSLGRLVRAKNTEQGVITHPSNVAPLTDPLT
                     GNNGWSSAYSYDANGNLTARTDSRLVSMGVHVTATNEYDNINRLKQTTYNNGTPFTLR
                     TSDFAPTNGRGRFYADYESSTSGTLNYVNAYDPMGRPTSGQTEFYLAGTGWRPAYTYS
                     RTYDLIGNVTKQTYPSTRTVNYTQFDMAGRLKQFNGNLGDGTPRTYSTNIEYDEASRL
                     LQEQFATQTPLYNKLEYNARGQKTAVRLSTLSRQAAYFNWNRGALIFDYGTTANNGNL
                     VSQQNWVPFTVANDEVTSGTSYQQGYSYDSLNRLTLVQESRLNEGFQFQQGYQYDRWG
                     NRRINQSTTTQTLPPEMRREFTVDAQTNRLGVPGGQMGVMAYDQAGNLNHDSYTGRGD
                     RTYDAENRMTSAVIGINSSSYYTYDAAGKRVRRQNSNEAAVWQVYGFDSELLAEYAAN
                     AAPTSPQKEYGYRNGELLVTATAPASAAANHNAVSAFSASQNPTGAWSYGYKPAGGAF
                     TLLMGTSTYFGAGTNTWALPGQACCPMVTKNNTASNYPYVNASTVVQPPDTLNLHPGA
                     SGERSVVRWTAPAPDTYTISGRFQGIDTYGTTSDAIVAHNGATIFGGAVNGHGAQAPF
                     SVTRTVAAGDTVEFSVGVGANGTYDSDSTGLAATITAQGGQADLKWLVTDHLGTPRMV
                     VDLSGNLDKIKRHDYLPFGEELTNQGGRTSGEGYAGDHVRQQFTGYERDDETRLDFAQ
                     ARYYASAQGRFTSIDPLFMQAEMITDPQRFNLYAYVSNNPLVFVDPTGESIRLEGTAE
                     ERAAMLKALQDAVGEKAARYLYINPCEIKDSGGNVIRTDYYVGVYTGMDGGKAPAFEQ
                     INDVAADFGAIIKDEKTVSIGFADAGKITEERGDVVNLGKFGASLTSTPGITGKFGGE
                     TGIKIVRSRDGNYGTYPGDAMEGGQDDQLTLSIILGHELSHAKHAFSLGRISEKHEGG
                     EPMAINFENKVRNIQNKPARKF"
     gene            complement(5544..6617)
                     /locus_tag="VGB76_14385"
     CDS             complement(5544..6617)
                     /locus_tag="VGB76_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100134.1"
                     /translation="MSLSVPLGGVPGRAGASLPISLRYSSKLWRLEGGAPFTNTGGIK
                     HTWAYPKYSENSMAGWTSSLEPPRIEFTGEEQLFGSTGQPLGGGIDDQESYGYYVRRI
                     HLHLPDGSSHELRKDDQTPGFPLDNPVYDFSGMYYAVDGSRTRYDYDANSDTGVLYLP
                     DGGRYHFGARQTVTRYGHAQERSWGTTYIDRHGNTMTYNMGTGVWTDTLGRTITNPLF
                     LDPQANEVRPYQVPGINGQPLIYKLHWKLLADALRVSGALSYVSNTRCVSPTTYQSIS
                     PYLFTSGGNNVHVCTPTGAGGNPVAFNPVVLAKIELPNGQFYDFKYNVWGEIEQMIAP
                     GGGYERYSYAAVASLSRAISPYS"
     gene            complement(6934..7827)
                     /locus_tag="VGB76_14390"
     CDS             complement(6934..7827)
                     /locus_tag="VGB76_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100135.1"
                     /translation="MSDRKYIPAIPRARLTAIFVSLILSAAIVGSLTVWSALARQRGQ
                     EEAMLLSPASLPSAENDPAAASGAGRDAREPSSGAPPALQSISPSAFTISWERTETSA
                     DGHSRLLRTTTRYQRSDGLYKLVQIYPAREDAAGHVETYYGYVGLGLFRLDETQGRLV
                     FVASQSDERSEDVEAALRSDPRFDREEDVRGQRALVMRTVGEDEAAYKEEFHAPALGG
                     LLVRRVEHSAIRREVWEPTGIEFGEPAASLFADIDRNRPDYTHYEREIQKTQRDPQQR
                     RAAQVMRELMARMRAVRPDAR"
BASE COUNT         1531 a         2399 c         2471 g         1814 t
ORIGIN      
        1 cgagagtgcg gatagctcca caggcggggc tgtaatgatt ggataacggt cagctagaga
       61 ggttcggctt accatgttcg gtcttaggtc ttgtcctaac cgataaccct tggcttggct
      121 aaatgaaggg agtgcaatta ccaacataat gagaacaaaa aactttctca tctgacagct
      181 cctttcggcc tatgtccttg ttggtaattt ctacctatcc cactattctg tcaatcaaca
      241 aaacaggtaa aagaatcacg tcttattgta gaacttggac gccgcctgac atcaagtttg
      301 aagtgcaaca ctgactaaaa ctcgttgaag cattccagcg gtgtcctgaa cccgagccgc
      361 ttcggtggtc gcgtgttgag cttgagaagt gtgctactcg gaaagttcct cctcaacaac
      421 cagaacatag aaggttccat catactgttc gcgcaaggtc gtatcgaaat agatagctgt
      481 gttgagagca ttatagatta cccctttata ctttttatca cgaaacctga tcatgaatac
      541 cggatcaccg gcgtatgcaa gaatgcagca gcttgcctgc tcaatactcg cgccattatc
      601 agtcaggcga ctttgaataa gctcgaacat actttcgccc ttttttcttt tcgctctgat
      661 gaaaacatac tgatgcccct tagcgagccg gtcaggcagc gtcttgccag acaactcttc
      721 tttgggaata tgtggtagcg cagacggatc gaacccacct tcgcctatcc cgacatatgt
      781 gaaatcaaag agagttttaa gagcagcagg ttcagtcgta ttattctgat ggttgctagc
      841 gaagatgaag gtgacgcaac ctacaatcaa aagtagaagg gcaagcgggg ttgccgaatg
      901 ccgacatgga aagccgtgcc attttagctt cataactctc acctcaacct ttcggagaac
      961 cactaaaact ttcgcgccgg tttattttgg atattgcgca ctttattttc aaagttgatg
     1021 gccatgggtt caccgccttc gtgcttctcc gatattctac ccagactaaa tgcgtgtttg
     1081 gcatgggaga gttcgtgacc aaggattata ctaagcgtaa gctggtcatc ctgcccacct
     1141 tccatcgcat ccccagggta ggtaccataa ttgccatccc tgctacgaac gattttaatg
     1201 ccggtttctc cgccgaactt tccagtgatt cccggtgttg acgttaggga tgctccgaac
     1261 ttccctagat tcacgacgtc tcccctctcc tcggtgattt tacccgcgtc tgcaaagccg
     1321 atcgagacgg tcttttcatc tttgattata gcgccgaagt cagccgctac atcgttgatc
     1381 tgctcgaatg ccggagcctt tcctccatcc ataccggtat aaacgcccac gtagtagtct
     1441 gtcctgatta cattaccccc actatctttt atttcacatg gattgatgta taagtacctt
     1501 gcggcctttt cacctacagc atcctgtaat gctttgagca tggctgcccg ctcttcagca
     1561 gtgccttcca gtcgtataga ctcaccggtg ggatcaacaa aaaccagagg attgttactc
     1621 acataagcat aaaggttaaa tctttgaggg tctgttatca tctcagcttg cataaagagg
     1681 ggatcgatgc tcgtgaacct cccttgcgca ctcgcatagt agcgcgcctg cgcgaagtct
     1741 aacctcgtct catcgtcccg ctcatagccg gtgaactgtt gcctgacatg atcccccgca
     1801 taaccctccc cgctcgtcct cccgccttgg ttcgtcagtt cctccccgaa aggtaaataa
     1861 tcatgccgct taatcttatc cagattcccc gacaggtcta ccaccatgcg cggcgtgccc
     1921 agatgatccg tcactaacca tttaaggtca gcctgcccgc cctgcgccgt gatagtcgcc
     1981 gccagccccg tgctgtcgct gtcatacgtg ccgttggccc ccaccccgac cgagaactcc
     2041 accgtgtcgc ccgccgccac cgtgcgcgtc acggagaagg gagcctgcgc gccgtggccg
     2101 ttgaccgcgc cgccgaagat cgtcgccccg ttatgcgcta cgatggcatc ggaggtcgtg
     2161 ccgtaagtgt cgatgccctg aaagcgtccg gagatggtgt aagtgtcggg cgcaggcgca
     2221 gtccagcgca cgacgctgcg ctcgccgctc gcgcccggat gcaggttgag cgtgtcgggc
     2281 ggctgcacca cagtcgaagc gttcacgtag ggataattcg aggcggtgtt gttcttagtc
     2341 accatcgggc agcaggcctg accgggcagc gcccaggtgt tggttcccgc gccgaagtag
     2401 gtcgaggtgc ccatcaggag cgtgaacgcg ccgcccgcag gcttgtagcc gtagctccat
     2461 gccccggtcg ggttctgcga tgctgagaag gcggagacgg cgttatggtt agccgccgca
     2521 gaggcgggcg cggtggccgt caccagcaac tcgccgtttc tgtatccgta ctctttctgc
     2581 ggcgacgtgg gagcggcgtt tgcggcgtac tctgcgagca actccgagtc gaagccgtag
     2641 acctgccaga cggccgcctc gttgctgttc tgccggcgca cgcgcttgcc cgccgcgtcg
     2701 taggtgtaat aactcgaact gttgatgccg atgacggcgc tcgtcatgcg gttctccgcg
     2761 tcataggtgc ggtcccccct gcccgtgtag ctgtcatggt tcaggttgcc cgcctggtca
     2821 taggccatca cgcccatctg cccgcccggc acgcccagac ggttggtctg agcgtcgacc
     2881 gtaaattccc ggcgcatctc cggcgggagc gtctgcgtcg tcgtactctg attgatcctg
     2941 cggttgcccc aacggtcgta ctggtagccc tgctgaaact ggaatccctc gttgagccgc
     3001 gactcctgca cgagcgtcag acggttgagc gagtcgtaag aatagccctg ctgataactg
     3061 gtgccggagg tgacttcatc gtttgcgacc gtgaaaggca cccagttctg ctgcgacacc
     3121 aggttgccgt tattcgcggt cgtgccgtag tcgaagatga gcgcgccgcg gttccagttg
     3181 aagtaggcgg cctgccgcga cagcgtgctc aggcgcacgg cggtcttctg cccgcgcgcg
     3241 ttgtactcca gtttattgta gagcggcgtc tgggtggcga actgttcctg gagcaggcgg
     3301 ctcgcttcgt cgtactcgat attcgtggag taggtgcgcg gcgtgccgtc gccgaggttg
     3361 ccgttgaact gcttgagcct gcccgccatg tcgaactggg tgtagttgac tgtgcgggtg
     3421 gacgggtaag tctgcttcgt gacgttgcct atcaagtcgt aagtgcgcga gtaggtgtaa
     3481 gccggtcgcc aacccgtccc tgccaggtaa aactcggtct ggcccgacgt gggccgcccc
     3541 atcgggtcgt aagcattcac atagttgagc gtgcccgatg tgctcgactc gtagtcggcg
     3601 tagaaacggc cacgcccgtt cgtcggcgcg aaatcagatg tgcgcagggt gaatggagtg
     3661 ccgttgttgt atgtggtctg cttgagacgg ttgatgttgt catactcgtt ggtggccgtg
     3721 acatgcacgc ccatgctcac gagccgggaa tcggtgcgcg cagtcaggtt gccgttggcg
     3781 tcgtatgagt aagccgatga ccacccgttg ttgcccgtca aagggtcggt cagcggtgcc
     3841 acgttcgagg ggtgggtgat cacgccctgc tccgtgttct tcgcccgcac gagacgccct
     3901 aatgaatcat acatgaagaa gcggagctgc ccgccctgat ccactttgcg caggttcccc
     3961 agcacgtcgt acagataact cgtctggtag gccagccccg cagggtcttc gacgacctgc
     4021 gtcaacctgc cgagcgcgtc cgtcacgctc ctcctgacct tgcccgactg gtccgtgacc
     4081 gtcacctgca cgccgctgta gctggtactc accaccgcgc tgtccggggt cgtcaccgtc
     4141 aagacccgcc cgagcgcgtc atacgccgtc gtagtccaac gtccggaggg gttgatggcc
     4201 gatcccacgc ccgcgctgag gtacgggttc gagacgcgcc acacccgccc catcgcgtcg
     4261 tactgggtat cggtagtgct cgactggctg ccttccgacc ggaaggagcg cacgcttctc
     4321 cccagcccgt cgaagtaatc gtaaccgtct gtggtctgcg tcgcgttgat ggccgtgcgg
     4381 gtgcggacat aatcgccgta agcgttacgc ccgtattcat aagccgtcca cccgccgtcg
     4441 ggctgttcca ccttacgcag cctgtcgagc gcgtcccgga cgacgttgcc gtattggtca
     4501 tggtaagtgt acttgagaac ggtcgtctta gagttggcat ccgtggtgga agtgatctta
     4561 cccgtcgaga aatcataggc catccacgat tccagcggag tgttggtggc gcggttattc
     4621 gtcgggtctg gaacggaaga ttttgtgtgc gtggggtagg cgtagtggta agcggaagaa
     4681 tattccacct ccgacacgtt gcctttagcg tcccacgcgt aacgagggtt gccgacctga
     4741 tcgtagcggg cgtgcgtctc tatccagcta ttcgaggtgt cgagccaact cctcgtcgtc
     4801 gtcacattcc cgcgcacatt tgtgcccggg tctgtccagc ccacgaccgc cccgtaggag
     4861 ggtaagagag agtaggccgc ctcgtcgtac ttcacctcgc tctgcgcgac gactgtgtta
     4921 actgtgttag cggcagtctt gacacgcgtc gaagagggca gcccgaccat gttgcgcgcg
     4981 cggtaagcat cccgcgtcgc agccgagatg ttcgcgtcgt tgaccagata ggtcgtctcg
     5041 tccgtgcgca ggagtgtccc accaggtatc tcacctatcg cgcctgtctg cgccgtcgtt
     5101 tgatcaactg atgagtagtc gtagctatgc ttcgccgtca cgttcaggtc ggcgtcatag
     5161 accatcatcg tggtggagga gagcgcggtc ccgcccgtgt ccagaatgag acctacggtt
     5221 ttagtcacgt gcgggtcgcg cgggcgggtg ctgcccgaag gcgtaatcgt tgaccatccc
     5281 gtcagcgtgc gccgcaacat ctggttcgac gagttataga cgcgctcgtc atagctcatc
     5341 cccgccaaag gcgtctcgaa gccgtactgc gtgccgaagc tgttggcata tatcagccgc
     5401 tcggagcgtg tgccgtccgg ctccgtcgtc gtcgccgtcg cgaagcccgt gctcttcgtg
     5461 atggagtacg accagtgatg tgtcgcctcc tcgacgcccg tcccgctcgg gctgacccag
     5521 cgttcgatga ctccgcggtt tgactagctg tagggcgaga tggcgcggct gagcgaggcc
     5581 accgccgcat agctgtaccg ctcgtagcca ccgccgggag cgatcatctg ttcgatctcg
     5641 ccccagacgt tgtacttgaa gtcgtaaaac tgcccgttcg gcagttcgat cttcgccagc
     5701 actacggggt tgaaggccac ggggttgccg cccgcacccg tcggggtgca gacgtgtacg
     5761 ttattgccgc cgctcgtgaa caggtagggc gaaatcgact ggtaggtggt gggcgacacg
     5821 cagcgcgtgt tcgagacata gctcagcgcg ccggagacgc gcagggcatc ggccagcaac
     5881 ttccagtgga gcttgtatat taaaggctgc ccgttgattc ccggcacttg atacggtcgt
     5941 acttcgttgg cttgcggatc aaggaagagg ggattggtga tggtgcgtcc gagcgtgtcc
     6001 gtccacacgc ctgtgcccat gttgtaggtc atcgtattgc cgtgccggtc gatgtaggtc
     6061 gtcccccaac tcctttcctg agcgtggccg tagcgcgtaa cagtctgccg cgcgccgaag
     6121 tggtagcggc ctccgtctgg caggtagagc acgcccgtgt cgctattcgc gtcgtaatcg
     6181 tagcgcgtgc gcgagccgtc caccgcgtaa tacatgccgg agaagtcgta gaccgggttg
     6241 tcgaggggaa aaccgggcgt ctggtcatct ttacgcagtt cgtggctgga gccgtctggc
     6301 aggtgcaggt ggatgcgccg cacgtagtag ccatagcttt cctgatcgtc gatgcccccg
     6361 ccgagcggct gtccggtcga gccgaacagt tgctcctcgc ccgtgaactc gatgcgcggt
     6421 ggttcaaggc tgctcgtcca accggccatc gagttctcgg agtacttggg gtaagcccac
     6481 gtgtgcttga tgccgcccgt attggtgaaa ggcgcgccgc cttccagccg ccacagcttc
     6541 gacgagtagc gcagggagat gggcagagat gctccggcgc gtcccggcac accgccgagc
     6601 ggcacggaga ggctcatccc gagggtcgag gggtcaacgc gcgcgcatcg gctttgagcg
     6661 cctggtcggg cttattcttg ttgtactcct gcgccgccgc agagagcgcc aagcagagca
     6721 caacagcgag cgcggaggat aagcgcagga cgcgacgacc tacaacggcg atagaggcgg
     6781 tggaggcgtt gagcatgccg acaggatttc tgaacaggtg aggggactga gtagggcgca
     6841 tcggaaacat gacctcgtga ggggtacgga tgaaagccgt ccaagttggt ctgcaggata
     6901 agccgcgggt gcagagagag agagtcgcct gcttcagcgc gcgtccggcc tgacggctct
     6961 catgcgcgcc atcaactcgc gcatcacctg tgccgcacgg cgctgctgcg ggtcgcgctg
     7021 cgtcttttgt atctctctct cgtaatgtgt gtaatcgggc cggtttcggt ctatatcggc
     7081 gaagagggaa gcggcgggtt cgccgaactc gatgcccgtc ggctcccaga cttcgcgcct
     7141 tatcgccgag tgctcgaccc gcctgacgag caggccccca agcgcgggcg catggaactc
     7201 ctccttgtag gccgcctcgt cctcccccac tgtgcgcatg acgagtgcgc gctgtccgcg
     7261 cacgtcctcc tcgcggtcga agcgcgggtc ggagcgcagg gctgcctcca catcttctga
     7321 ccgctcgtcg ctctgggatg cgacgaagac gagccgcccc tgcgtttcgt cgaggcggaa
     7381 gaggccgagc ccaacgtaac catagtaggt ttcaacgtgg ccggcagcat cctcgcgcgc
     7441 cgggtaaatc tggacgagct tgtagaggcc gtcagagcgt tggtatcgag tggtggtgcg
     7501 cagcaggcgc gagtgcccgt cggcggaagt ctcggtgcgc tcccatgaaa tggtgaaggc
     7561 tgacgggctg attgattgca gggccggcgg cgcaccactt gatggctcac gcgcgtcgcg
     7621 tccggcgccg ctcgcggctg cggggtcgtt ttctgccgac ggcagcgagg ctggagacag
     7681 aagcatcgcc tcctcttgtc ctcgctgacg cgccagtgcc gaccacacgg tgagcgagcc
     7741 gactatcgcg gccgacagga tgagcgagac aaagatggcc gtcaggcgcg cccgagggat
     7801 ggcgggaatg tatttgcgat cagacatagt ctttttactt cggaggcggt ttaactgctg
     7861 acatgggtga ggggaatgtt ccttcggaga ttccacggcc gtgtcggcgc agtgattaac
     7921 acctacgccg gatgtcggag catgagtcgg ctaccggatg aaaaagtgat ggacgggata
     7981 atgacgccgc cgtcaatttt cgtttgtggg ttgaaagtgg cagcggttga ggccccgcag
     8041 catcgggcgc gagaaacgaa agtggagaat aagagacggc gatatatttg tcaatttgtg
     8101 ccggatctgg ggattgccta aattagacct gatgatgata taagactcga catgcatcaa
     8161 tgtcctcatt gccacagtag cgagcgccaa gtgaaatcag gctttaaccg caccg
//
LOCUS       DASWNS010000417        13276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_67585, whole genome shotgun sequence.
ACCESSION   DASWNS010000417 DASWNS010000000
VERSION     DASWNS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13276
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67585"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1485
                     /locus_tag="VGB76_14395"
     CDS             64..1485
                     /locus_tag="VGB76_14395"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY0100136.1"
                     /translation="MSAPNIETIVSLAKRRGFVFPSSEIYGGLGSAWDYGPLGVELCN
                     NVKRAWWRWMVHERDDMEGLDSSIILNRSVWKYSGHEETFSDPLVDCKECNSRFRADK
                     LEFDGAAHANVEAALRAGQVKCPNCGSTKLTEPRPFNLMFRTQLGAASAEDDPNALAY
                     LRPETAQGIFINFHLVQQTMRRKVPFGIAQIGKSFRNEVTPGNFIFRTREFEQMEMEY
                     FVRPGEDEAVHQEWIDNCFNWYVSLGLSRDNLRLYEQPKEELAHYAKRTVDIHYRFFP
                     ERAEEERHWDELMGIANRTDFDLRAHSKNPVDAEGKRLNPDSTEDLSYFDEARKERYY
                     PYVIEPAAGVNRSVLAFLMDAYSERTNDKGETRVILKLHPELAPIKVAVLPLAKNKPE
                     IVRLAKAIKRTLQPSMRAVYDDTGGIGKLYARQDETGTPFCVTVDHQSLEDEAVTVRD
                     RDTWEQERVKVSDLQEHLRKRLV"
     gene            1517..1792
                     /locus_tag="VGB76_14400"
     CDS             1517..1792
                     /locus_tag="VGB76_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100137.1"
                     /translation="MLINGRNFLLDFDGQARRVGFYATRTVEAATADEAESAAVELLR
                     SDELLRQSTLNEQTDPPMMYVEEIVEVRRRKKGEDANTGFSFYNESE"
     gene            1799..2260
                     /locus_tag="VGB76_14405"
     CDS             1799..2260
                     /locus_tag="VGB76_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100138.1"
                     /translation="MAIRKQNTQRTDARLLLESDEPVELPPAEIEESAVIVQFDNVKP
                     FIPELLETLREGTIAELVPVMLDGSTFAIHTRTRTFWVKLWHANNSEGDLSRIERAQV
                     LSCLPSTRGLQVIVGEEDEEDFADEEFDEDDFDETDDTDEGGAPVNPQPET"
     gene            2300..3688
                     /locus_tag="VGB76_14410"
     CDS             2300..3688
                     /locus_tag="VGB76_14410"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0100139.1"
                     /translation="MNRVPAKIGALAVAVREAGGRALLVGGCVRDELMGREPKDWDVE
                     VYGVEPSALRALLEKFGRVDAVGEAFTVYKLGHDLDVSLPRRERKEGRGHRGFVIEGD
                     PTMSVAEAARRRDFTINAILQDPLTGETLDPYDGQVDLRRRTLRAVSRETFAEDSLRV
                     LRAAQLAARFDFDIEAETVELCRTIDLSDLPAERVWGELEKLLLRAARPSVGFRWLAR
                     LNATPQLFPEIQALSGVPQEYDWHPEGDVDVHTWLTIDRARELIDDLPYAKGVTVMLA
                     ALCHDFGKPATTEFVEGRTRSRGHDEAGVAPTNAFLDRLKIFTLDGYDVRAQVCELVR
                     AHLKPGEFYHKRAEVSEGAFRRLARRCELDLLYRVARADTLGRNADWVPRERWFDAVP
                     QEWFIARARELAVEERPPAPLLLGRHLLALGLKPSPRIGEITRAVYEMQLDGRVRTLE
                     EAEAAARDLLGG"
     gene            4180..6312
                     /locus_tag="VGB76_14415"
     CDS             4180..6312
                     /locus_tag="VGB76_14415"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEY0100140.1"
                     /translation="MINMQVIQETEKRYSERKSPRDATARKIETKPLHQIDEPERVEK
                     RMERLGVAPETARAFSVGEVVSRESVPPEGVTDIEGAAMERILGRSDLLSISYLEQGV
                     AVSRSIGRVRIRNDRGRVVGYGTGFMVSPRLLLTNNHVLRNTQEASFSQVEFNFQDGL
                     DGRPLSSIVFDLDPATFFITDRALDFSVVAVRDQSSGAASLASFGWSRLIEAEGKILL
                     GEYASIIQHPNGEPKQLALRENQVIDLLDNFLHYKTDTSPGSSGSAVFNDQWEVVALH
                     HSGVPQRDTQRRILSTSGALWTEDMGEQKVAWIANEGVRISRIVKHIKNQNLSTAQRQ
                     LRTEMFETAPPPLSLRRDEVSSPQTVNRPGEVAVSSSPSSPANMSWTFPFTISVQLTP
                     SGVAAQVDQSGVSAGAVSPSAPVVRDERPSDDPDLREALAELEAARARPYYDETRDRK
                     DRTTYYKGLPGTLAPANLYAALNELLTSTHANKPKYQPSRHVYPWVDLQPNLKLRSIY
                     SAKEFDPEEFIREDFRIDQERAERFREALSVESFTGAERMAEELDLLEASLPYNCEHV
                     VPQSWFGKREPMRGDIHHLFACESGCNSFRSNIPYFDFTDFEEAIRDECGKRETNKFE
                     PNAGKGTVARAVLYFLLRYPGEINQIETEYDAERLKVLLAWHRANPVTEYERHRNMAI
                     YAAQGNRNPLIDFPSWAAKIEFKLGLGK"
     gene            6534..7769
                     /locus_tag="VGB76_14420"
     CDS             6534..7769
                     /locus_tag="VGB76_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0100141.1"
                     /translation="MIDFELTEDHKSIRNTVREFAQREVAPRIKELDEQQRFDRSILD
                     KMAELNLLGVCIPEEYGGAGFDYIALGLVCEELEAVDTFMRVIMSVHTGLNSMTLYTW
                     GTEEQKRKYLVPQAKGEKIATYGLTEPGAGSDVVGARSTGRREGDEWVLNGEKMWISL
                     AGDVADNFLFFCWTDEEKRRKRDHTGMSCFIVERSMQGFTSGTIHGKMGIRAGNTGYF
                     SLQDVRVPAENLVGEEGEGFKIAMFALEQGRYTVASGATGIIRASRDASIAYANTRET
                     FGTKIGNHQLVKQKIAEMEADYQMSHLLWLRAGYLKNEGKPNAKETSLAKWQATVRSE
                     KAASMAIEVHGANGFSNDYPVERYLRNCKAAIIYEGTRDIHTLMQADWALGLKKEKAA
                     RKTLPPYRPAEAREAVGAD"
     gene            8028..9086
                     /gene="glpX"
                     /locus_tag="VGB76_14425"
     CDS             8028..9086
                     /gene="glpX"
                     /locus_tag="VGB76_14425"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY0100142.1"
                     /translation="MGYEIKTEHLSLDFLRVCEAAAIEAAKTMGQGDRKYSDHVAVEA
                     MREVMDTVPMLGRIVIGEGERDEAPMLYIGEEVGQGVRAVREKGEQCDEVYPEVDIAV
                     DPLEGTNLCALGANNAIAVLAASERGGLLNAPDIYMDKIVVGPSSRGRVDINAPVKDN
                     LQAIAKGLGRDVDDLTVIVLDRTRHKKLIQDVRDAGARIRLISDGDLSAGISAAVAGT
                     NIHALVGIGGAPEGVITAAAMKCLNGEIQARLVFDPERLDVDKNKVPPQEEVVERLKE
                     MGIDDPNKVYSTDDLAPGKKIIFAATGVTDGALLRGVRFFGAGKRTHSLVMTTEARHI
                     RFVDTVHVEGGPDTVIRF"
     gene            9194..9778
                     /locus_tag="VGB76_14430"
     CDS             9194..9778
                     /locus_tag="VGB76_14430"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEY0100143.1"
                     /translation="MPASPDSYISPLAPLPPGAFSFQRGAWACVVACTLLWAGLILGA
                     PVAAAGGHETLAFVIYRGLDGVCHQMPERAFWIAGHPLAVCARCSGIYAGFTLAALLY
                     PLVARRLPASVTPRREWLILALAPTLIDFTLGITGLWANTHLSRSLTGAWLGAWLAFY
                     VVPGVLEIARQRGRRQLQRSASHGAAAAVAFEQK"
     gene            9915..10436
                     /locus_tag="VGB76_14435"
     CDS             9915..10436
                     /locus_tag="VGB76_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100144.1"
                     /translation="MNEQTKPAIMAGLGIAAVMIVLSLIGTVVPFAGCCNCLLPIAAG
                     VLAVYLYAKNSSAPLTPADGAKLGAIAGVAGGLLNLIIGTPLSYFVNAAVMEAQFAQL
                     RDQGVSLPASLTGLALFIVMGIVGAVIFVILAAIGGLIGAAIFGKNRPGGVATPPPPP
                     PPSYGGGGGGFGS"
     gene            10589..11182
                     /locus_tag="VGB76_14440"
     CDS             10589..11182
                     /locus_tag="VGB76_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100145.1"
                     /translation="MSEKTRAALIGGVTLGLLSAIPPISLANMCCCVWVLGGGALASY
                     LYVRRSPTPVLLGQGAELGALTGVVGTIVSHSIGIPLGLILGDVFNEFLVNMFENLNP
                     QAGEEVRRAVEAAQAQTFVQKLPVILGTAVFNTVIYIAFATLGGLLGVKLFEKRLNTL
                     DNTPPPPPSDFGGSQPPPPASPPTTGGSGYGSFGSGN"
     gene            11397..13025
                     /locus_tag="VGB76_14445"
     CDS             11397..13025
                     /locus_tag="VGB76_14445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0100146.1"
                     /translation="MSKTPEELWREYGALQSDFVDAWHWRTQSNISVLYELDAFDWTV
                     LILYFSILFVLAIYGAYRIKQVADFWRYRHIQPAPKARFAESDLPVITVQLPLFNEMY
                     VVERLLRAVTQFDYPRERLEIQVLDDSTDETVALARRTVEAYRAEGFDIHYIHRSDRT
                     GFKAGALANGTQTAKGELLAIFDADFVPKPDCLRKLVDYFTDPLVGCAQMRWSHINGA
                     YNLLTRLQTIMLDGHFVIEQTVRNRTGGFFNFNGTAGIWRRRAIELSGGWQHDTLTED
                     TDLSFRAQLMGWRFVYLLDEDAPSEIPVEINAFKAQQRRWAKGVMQVGIKLYPRIWRA
                     NLPLRVRLEMFFRLTGNISYPLMIVASFLQFPLLLVRYNQGFYHLMAFDVPLLFFSTV
                     SVILFYGSAIWYLDKATGRGRRLLHLPLVMAVGIGLAFSNARAVIEALMGVKSEFVRT
                     PKYQVEATSDETWKAKKYKRKRGLLPLLELSFAAYFFCAVWYAAHMSMWGTIPFLFLF
                     FFGYGYIGLMSILQTAGGKRFQLWRRPLAIGRRA"
BASE COUNT         2625 a         4093 c         4029 g         2529 t
ORIGIN      
        1 ttttctattc acgattgacg attcacgatt cacggctttt cgacgattta cggaggttaa
       61 catttgtctg caccgaacat cgaaacaatc gtcagtctgg cgaagcgacg cggcttcgtg
      121 tttccctctt cggagattta cggcggcctg gggagcgctt gggactacgg cccgctcggc
      181 gtcgaactct gcaacaacgt caaacgcgcg tggtggcgtt ggatggttca cgagcgcgac
      241 gacatggaag gactcgattc ctccatcatc ctcaaccgct ccgtctggaa atattcgggg
      301 cacgaagaga cgttcagcga tccgctcgtc gattgcaaag agtgcaacag ccgttttcgt
      361 gccgacaaac ttgaattcga cggcgcggct cacgctaatg tcgaggccgc gcttcgagca
      421 ggacaagtta aatgtccgaa ctgtggctcg actaaactaa ccgagccgcg ccctttcaac
      481 ctgatgtttc gcacgcaact cggcgcggcg tcggcggaag acgacccgaa cgcgctcgcc
      541 tacctgcgtc ccgaaacggc gcagggcatc ttcatcaatt ttcatctcgt gcagcagacg
      601 atgcggcgca aagttccgtt cggcatcgcg cagatcggaa aatcgtttcg taacgaagtg
      661 acgcccggca acttcatctt ccgcacacgc gagtttgagc agatggagat ggaatacttc
      721 gtgcgtcccg gcgaagacga agccgtgcat caggagtgga tcgacaactg cttcaactgg
      781 tacgtgtcgc tcggcctcag ccgcgacaac ctgcgtctct acgagcagcc gaaagaggaa
      841 ctcgcgcact acgccaagcg caccgtcgat attcattatc gtttcttccc cgaacgcgcc
      901 gaagaagagc gacactggga cgaactgatg ggcatcgcca atcgcaccga cttcgacctg
      961 cgcgcgcact cgaagaaccc tgtggacgcg gaaggcaaac gcctcaaccc cgattcgacc
     1021 gaagacctct cctatttcga cgaggcgcgc aaggagcgtt attaccctta cgtcatcgag
     1081 cctgcggcgg gcgtcaatcg ctccgtgctc gccttcctga tggacgccta ctccgagcgc
     1141 acgaacgata agggcgagac gcgcgtgatc ctgaaactcc atcccgaact cgcgccgatc
     1201 aaggtggccg tgttgccgct cgccaaaaac aagccggaga tcgtccgtct ggcgaaagcg
     1261 atcaagcgca cgctgcaacc ctcgatgcgc gccgtctacg acgacacggg cggcatcggc
     1321 aaactctacg cgcggcagga cgagacgggc acgcccttct gcgtcaccgt cgatcatcaa
     1381 tcgctcgaag acgaagccgt gaccgtgcgc gaccgcgaca cctgggagca ggagcgcgtc
     1441 aaggtgtcgg acttgcaaga gcacttacgg aagcggctcg tttagaattt cagtcaagcg
     1501 tatgtcaagg tttcgagtgc tgatcaacgg aaggaacttt ctactcgact tcgacggtca
     1561 agcacggaga gtaggtttct acgcgacgcg gacagttgaa gccgcgacag ccgacgaagc
     1621 ggagtctgcc gccgtcgagt tgctcaggag cgacgagctt ttgcggcaga gtacgctcaa
     1681 cgaacagacc gacccgccga tgatgtacgt tgaagaaatc gtagaagtca ggcgtcgcaa
     1741 gaagggcgaa gacgcaaaca cagggttctc tttctataac gaatccgaat aacaagagat
     1801 ggcaattaga aaacagaaca cgcagcgcac ggacgcgcgt ttgctgctcg aatcggacga
     1861 gccggtagag ttgccgcccg ccgagatcga agagagcgcg gtcatcgtgc agttcgacaa
     1921 tgtgaaaccg ttcattcccg aactgctcga aaccctgcgc gagggcacca tcgccgaact
     1981 cgtccccgtc atgctcgacg gctcgacctt cgccatccac acgcgcaccc gcaccttctg
     2041 ggtcaaactc tggcacgcca acaacagcga aggcgatctc tcgcgcatcg aacgcgcgca
     2101 ggtgctcagt tgcctgccgt ccacgcgcgg cctgcaagtc atcgtcggcg aagaggacga
     2161 ggaagatttt gccgacgaag agtttgacga ggacgacttc gacgagacgg acgacacgga
     2221 cgaaggcggc gcgcccgtca acccgcaacc tgaaacctga accacaagtc tcgcgcctta
     2281 gtctcaagcc ttccgcacga tgaatagagt tcccgctaaa attggtgcgc tcgcggtcgc
     2341 cgtgcgtgag gcgggcgggc gcgctctgct cgtcggcggt tgcgtgcgcg acgagttgat
     2401 ggggcgcgag ccgaaggatt gggacgtgga agtttacggc gtcgaaccgt cagccttgcg
     2461 cgcgctgctg gaaaagttcg ggcgcgtgga tgcggtcggc gaagccttca cggtctacaa
     2521 actcgggcac gacctcgatg tctcgctccc gcgccgcgaa agaaaagagg ggcgcggcca
     2581 tcgcggtttc gtcatcgaag gcgaccctac gatgtccgtc gccgaagccg cgcgccgccg
     2641 cgacttcacc atcaacgcca tcctccaaga cccgctcacg ggcgagacgc tcgatccata
     2701 cgacgggcag gttgatctcc gacgccgcac cttgcgcgcc gtctcgcgcg agacgtttgc
     2761 cgaagactcc ctgcgcgtgc tgcgcgcggc gcaactcgcg gcgcgcttcg acttcgacat
     2821 cgaggcggag acggtcgagt tgtgccgcac gattgatctc tcggacttgc ctgcggagcg
     2881 cgtctggggc gagttggaaa agttgctctt gcgcgccgcg cgcccgtcgg tcgggttccg
     2941 ttggctcgcg cgtctgaacg ccacgccgca actgtttccc gaaattcaag ctctctccgg
     3001 cgtgccgcag gagtacgact ggcaccccga aggcgatgtt gacgtgcaca cgtggctgac
     3061 gattgatcgc gcacgcgaat tgatcgacga cctgccttat gcgaaaggcg tgacggtgat
     3121 gctcgccgcg ctctgccacg atttcggcaa gcccgcgacg accgagtttg tcgaagggcg
     3181 cacgcgctcg cgcggccacg acgaagcggg tgtcgcgccg accaacgcgt ttctggatcg
     3241 tctgaaaatc ttcacgctcg atggctacga cgtgcgcgcg caggtctgcg aactcgtgcg
     3301 ggcgcatctc aagccgggcg agttttacca taagcgcgcg gaagtttccg agggcgcgtt
     3361 ccggcgtctg gcgcgtcgct gcgaactcga tctgctgtac cgcgtcgcac gcgcagacac
     3421 ccttgggcgc aacgccgatt gggttccgcg cgagcgttgg ttcgatgccg tgccgcagga
     3481 gtggtttatc gcgcgtgcgc gtgaattggc cgtcgaagaa cgaccgcccg cgcccctcct
     3541 tctcggacgg cacctgctcg cgctcggcct taagccttcg ccgcgcatcg gcgagatcac
     3601 gcgcgccgtc tacgagatgc aactcgacgg gcgcgtccgc acgctcgaag aagcagaagc
     3661 ggcggcgcgc gatctgctcg gcggttagac cgcgccgggc gcatcttcga gcggcgcgcg
     3721 gcaaacgctc cggcggcctt aaatattctc ctccggcacg cctgcgacgg aagctatgtt
     3781 tctcgataaa tgagttgaca aggttagggt cttgccttag tctgcccgcg ttgaaggtta
     3841 ccggccagat tcgctcgcgg gcatgatcct catcaaacac tcaccacagg gcttgaggga
     3901 tgcggcttca ggaggttcgc gaagcgcctg ttaaccagca ccgacaggtt gcccgccctt
     3961 tcgggcaacg ccgaacagcg gctcgccgcc gccctgcacc gttcgcttca catcttcgag
     4021 tttcccgccg gtcgccgagg gagacgatct gcgtccgggg ctaaagctaa ggtcgcgcac
     4081 atcactcatg caggtctgtc tttcctgctg ccttaatctg caacgttgtt ccgcaaacgc
     4141 tgacattcaa aaactctcac caccggaggt aagtatgcca tgatcaatat gcaagttatt
     4201 caggagacgg agaagcgtta ttctgaacgg aaaagcccgc gcgatgcgac cgcccgtaag
     4261 attgaaacca aaccgctgca ccagattgac gagccggagc gcgtggaaaa gcgcatggag
     4321 cggctcggcg tcgccccgga aacggcgcgt gcgttctccg tcggcgaggt tgtgtccaga
     4381 gagagcgtgc cgccggaggg cgtaacggac atcgagggcg cggctatgga gcggattctg
     4441 gggcgcagcg atctgctgag catcagttat ctcgaacagg gcgtggcggt ttcgcgttcg
     4501 atagggcgcg tacgcatccg caacgaccgt gggcgcgtgg tgggctacgg cacggggttc
     4561 atggtttcac cgcgcctgct gctcaccaac aaccacgtcc tgcgcaacac gcaggaagcc
     4621 tctttcagtc aggtggagtt taactttcaa gacgggcttg acggtcgccc gctctcgtcc
     4681 atcgtcttcg acctcgaccc cgccaccttt ttcatcaccg accgcgcgct cgatttcagc
     4741 gtagtggcgg tgcgcgatca atcctccggc gcggcctctc tcgcttcctt cggatggagc
     4801 cgactgatcg aggccgaggg caagattctg ctcggagagt atgccagcat catccagcac
     4861 ccgaacgggg aaccgaagca actcgccctg cgcgagaatc aggtcatcga cctgttagac
     4921 aactttcttc actacaagac ggacacgtcg ccgggttcgt ccggctccgc cgtcttcaac
     4981 gatcagtggg aagtggtggc gctgcaccac tccggcgtcc cgcagcgcga tacgcagagg
     5041 cgtattctct cgacgagcgg cgctctatgg acggaagaca tgggcgagca gaaagtcgcc
     5101 tggatagcga acgagggcgt gcgaatcagc cgcatcgtca agcacatcaa gaaccagaat
     5161 ctctctacgg cgcaacgcca gttaagaacc gagatgtttg aaaccgcgcc gccgcctctc
     5221 tcgctccgcc gcgacgaggt atcttcgccg caaaccgtga accgtccggg cgaggtcgcc
     5281 gtctcttcgt cgccgtcgag tccggccaac atgtcctgga ccttcccttt cacgatcagc
     5341 gtccaactca caccctccgg cgtggccgcg caggtcgatc agtcaggcgt gagcgcgggc
     5401 gcggtgtcgc cgtccgctcc cgtcgtgcgc gatgaaagac cgtcggacga cccggacttg
     5461 cgcgaggcgc tggccgaact cgaagcggcg cgcgccagac cctattacga cgagacgcgc
     5521 gaccgcaagg atcgcaccac ctactataaa ggtctgccgg ggacgctcgc gcccgccaac
     5581 ctctatgcgg ccttgaacga actgttgacc tcgacacacg cgaacaaacc caagtatcaa
     5641 ccttccagac acgtctaccc gtgggttgat ctccagccca atctgaaact tcgcagcatc
     5701 tattcggcga aggagtttga tccggaagag ttcatccggg aggattttcg catcgatcag
     5761 gagcgtgccg aacgcttccg cgaggcgttg agcgtcgagt cgttcacggg cgcggaacgg
     5821 atggcggaag aactcgatct gctcgaagcg tcgcttcctt acaactgcga acacgtcgtc
     5881 ccgcagtcct ggttcggcaa acgcgaaccg atgcgcggcg acatccatca tcttttcgcc
     5941 tgcgagtcag gctgcaacag cttccgcagc aacatccctt acttcgattt cacggatttc
     6001 gaagaggcga tccgcgacga gtgcggcaag cgcgaaacca ataagttcga gccgaacgcg
     6061 ggcaaaggca cggttgcgcg cgcggtgctc tatttcctgc tccgttaccc cggcgagatc
     6121 aaccagatcg aaaccgagta tgacgccgaa cgcctgaaag tgctgctcgc gtggcacagg
     6181 gcaaaccccg tgaccgaata cgagcggcac cgcaacatgg cgatctacgc cgcgcagggc
     6241 aaccgtaatc cgctgatcga ttttccgagt tgggctgcga aaatagagtt caaactcggc
     6301 ctcggcaagt gatcgggcgc gcacgccgcg agagttgagc gtcgtgcggg aacgcggctt
     6361 gcgcggggct gaccctccgg cagccgcccg ccggagggca atttccgcct cgcccgtgcg
     6421 ctgttgtaag atgtggcgca gtattgattt catcatgctg cgccacatct cttatgcctc
     6481 gccgccgaat gttgccgcgc gcctgaaatt ctttgttgaa ggaaaaccgg agaatgattg
     6541 attttgaact gaccgaagat cacaagtcta tacgcaacac cgtgcgcgag ttcgcgcaga
     6601 gggaagtcgc cccgcgcatc aaagagcttg acgagcagca acgcttcgac cgttccatcc
     6661 tcgacaagat ggcggagttg aacttgctcg gcgtctgcat cccggaagag tacggcggcg
     6721 cgggcttcga ctacatcgcg ctcggcctcg tctgtgaaga actcgaagcc gtggacactt
     6781 tcatgcgcgt catcatgtcc gtccacaccg gactcaacag catgacgctc tacacctggg
     6841 gcacggaaga acagaagcgc aaatatctcg tgccgcaggc gaagggcgag aagatcgcga
     6901 cctacggttt gaccgaaccg ggcgcggggt cggacgtggt gggagcgcgt tcgacggggc
     6961 ggcgcgaggg cgacgagtgg gtcttgaacg gcgagaagat gtggatcagt ctcgcgggcg
     7021 acgtggccga taattttctc ttcttctgct ggacggacga ggagaagcgt cgcaaacgcg
     7081 atcacacggg gatgagttgc ttcatcgtcg aacgttcgat gcagggcttc acgtcgggca
     7141 cgattcacgg caagatgggc attcgcgcgg gcaacacggg ctacttctct ctgcaagacg
     7201 tgcgcgtgcc tgcggagaac ctcgtgggcg aagagggcga gggcttcaag atcgccatgt
     7261 tcgctttgga gcaaggccgc tacaccgtcg cttcgggcgc gaccggcatc atccgcgcct
     7321 cgcgcgatgc gtcaatcgcc tacgccaaca cgcgcgagac gttcggcacg aagatcggca
     7381 accatcaact cgtcaaacag aagatcgccg agatggaggc cgactaccag atgtcgcacc
     7441 tgctctggct gcgcgcgggc tacctgaaga acgaaggcaa gccgaacgcg aaggagacga
     7501 gcctcgccaa gtggcaggcg accgtcagga gcgaaaaggc ggcttcgatg gcgattgaag
     7561 ttcacggcgc gaacggcttc tcgaacgatt acccggtcga gcgttacctg cgcaactgca
     7621 aggccgcgat catctacgaa ggaacgcgcg acatacacac gctgatgcag gccgactggg
     7681 ctttgggact caagaaagag aaggccgcac gcaagaccct gccgccttac cgtccggcgg
     7741 aagcgcgcga agccgtcggc gctgattgag aaactaaaga cgggattcaa cgccacgtcg
     7801 aagacggcga gcgcggagga ctgcgaaaag tctccccgct cgccgttctc tttccagaga
     7861 gtcgaattaa agccatgacg agattctttg cgagcggaag caaagttcta taatttctcc
     7921 ccgctctctg ctagtatgac ggcgcaattt ccggagggcg cgaggcaact cgcagttttc
     7981 accgactcgc cgcaaatatc attataagat cgggagataa aagaggcatg ggctatgaga
     8041 tcaagaccga gcacctgtcg ttagacttcc tgcgcgtctg cgaggcggcg gccattgaag
     8101 cggcgaagac gatggggcag ggcgaccgca agtattccga tcacgttgcg gtcgaagcga
     8161 tgcgcgaggt gatggacacc gtgccgatgc tcggtcggat cgtgatcggc gagggcgaac
     8221 gcgacgaagc cccgatgctc tacatcggcg aagaggtggg gcagggcgtg cgcgccgtgc
     8281 gtgagaaggg cgagcagtgc gacgaggttt acccggaagt ggacatcgcg gtcgatccgc
     8341 tcgaaggcac gaacctgtgt gcgcttggcg caaacaacgc cattgctgtg ctcgctgcct
     8401 ccgagcgcgg cggcctcttg aacgcgcccg acatttacat ggacaagatc gtcgtcggtc
     8461 cctcttcgcg cgggcgcgtg gacatcaatg cgcccgtcaa agataaccta caggccatcg
     8521 ccaaaggtct gggacgcgac gtggacgacc tgaccgtcat cgtcctcgac cgcacgcgcc
     8581 ataaaaaact gattcaggac gtgagagacg ccggtgcgcg catacgactc atctccgacg
     8641 gcgacctctc ggcaggcatc tcggcggcag tcgccggaac gaacatccac gcgctggtcg
     8701 gcatcggtgg cgcgccggaa ggcgtcatca cggcggcggc catgaaatgc ctgaacggcg
     8761 agatacaggc gcgcctcgtc ttcgacccgg agcgtctgga cgtggacaaa aacaaggtgc
     8821 cgccgcagga agaggtcgtc gagcgtctga aagagatggg cattgacgac ccgaacaaag
     8881 tctacagcac ggacgatctc gcacccggca aaaagatcat cttcgccgcc acgggcgtta
     8941 ccgacggcgc gctcctgcgc ggcgtgcgct tcttcggagc gggcaaacgc acgcactcgc
     9001 tcgtgatgac cacggaggcg cgccacatcc gcttcgtcga taccgtccac gtcgaaggcg
     9061 gtcccgacac ggtgattaga ttctagggat gagggcggag ggatgaggga tgaataaaac
     9121 aaaagctaat tatctagctt gaatgttttc ttattcatcc ctcatccctc cgccctcatc
     9181 cctcccttag tttatgcctg cttcccctga cagttatatc tcgccgcttg cgccgctgcc
     9241 gccgggcgcg ttctcgttcc agcggggggc gtgggcgtgt gtcgtggcct gcacgctgtt
     9301 gtgggcgggc ttgatcttgg gcgcgcccgt ggcggcggcg ggcgggcatg agacgctggc
     9361 attcgtcatc tatcgagggc tggacggggt gtgtcatcaa atgccggaac gcgccttctg
     9421 gattgcgggg cacccgctgg cggtatgtgc gcgttgctcc ggcatctatg cgggcttcac
     9481 tctggcggct ctgctctacc cgctcgtcgc gcgtcgcctg cctgcgagcg tgacgccgcg
     9541 acgcgagtgg ctcatcctcg cgctcgcgcc gacgttgatc gatttcacgc tcggcatcac
     9601 ggggctgtgg gcgaacacgc acctgtcgcg ttcgctgacg ggcgcgtggc tcggcgcgtg
     9661 gctcgccttt tacgtcgtgc ccggcgtgct cgaaatcgcg cggcaaagag ggcgacgcca
     9721 actgcaacgc agcgcgtcgc acggcgcggc ggcggcggtc gcctttgaac aaaagtgaaa
     9781 taactacgta aacaagtcag gccaattgat aacttcggcg caagccattt tctcttcgat
     9841 agaagctctc tggttcgagt tgaaaatgta cgatccaagt tttatccaaa ctctccgcga
     9901 aggtgtctga atatatgaat gaacaaacaa aaccagcgat aatggccggc ctcggcattg
     9961 ccgctgtgat gatcgtgtta agcctgatcg gcacggtcgt gcctttcgca ggctgttgca
    10021 attgcctgtt gcccatcgcc gcaggcgtgc tggccgtcta tctctacgcc aaaaactctt
    10081 ccgctccgct gaccccagcc gacggcgcga aactcggagc catcgccggt gtcgccggtg
    10141 gcctgttgaa tctcatcatc ggcacgccgc tctcctattt tgtgaacgcg gcggtgatgg
    10201 aagcccagtt tgcgcaactg cgcgatcagg gcgtgagtct gcccgcgtct ctgaccggcc
    10261 ttgcgctctt catcgtgatg ggcatcgtcg gcgcagtcat ctttgtgatt ctggcggcca
    10321 tcggcggttt gatcggcgcg gccatcttcg gcaagaatcg ccccggcggc gtggcgaccc
    10381 ctcctcctcc tcctccgcct agctatggcg gcggtggcgg cggcttcggc agctaacttc
    10441 atacacggcg cgccggattt gcgaactaac cggcgcgccg acgcctgacg tagtgcgggg
    10501 cggacggcaa gcagtgtcgt ttttcggacg aagactctgc ttgccgtcat ccctgcgctc
    10561 tgccgacatt ctttcccggt aacgacttat gagtgaaaag acgcgcgccg ccctcatcgg
    10621 tggcgtcacg ctgggcttac tctccgcgat ccctccgatc agcctcgcca acatgtgttg
    10681 ttgtgtatgg gtgctcggcg gcggcgcgct ggcctcctac ctgtacgtgc gtcgctcgcc
    10741 cacgcccgtc ctgctcgggc agggtgcgga actcggcgcg ctcaccggcg tggtcgggac
    10801 gattgtgtcg cattccatag gcatcccgct cggcctgatt ctcggcgacg tgttcaacga
    10861 gtttctggtg aatatgtttg aaaacctcaa cccgcaggcg ggtgaagagg tgcggcgcgc
    10921 ggtggaagcg gcgcaggcgc agaccttcgt gcaaaagctc cccgtgattc tcggcacggc
    10981 ggtcttcaac acagtcatct atattgcctt cgccaccctc ggcggcctgc tcggcgtcaa
    11041 actcttcgag aaacgcctga acacgctcga taacacgccg cctccgccgc cgtccgactt
    11101 cggcgggagc cagccaccgc cccccgcctc gccgccaacg acgggcggaa gcggctacgg
    11161 cagcttcggc agcggtaact gaaacaccgt cggaagtttg ttagcacgcg gcggttcggt
    11221 tacaatgtgc gcgcttaagg ggcagcgtcc ggagaagggc gctgctcgtt ttatgtagac
    11281 ggcaggcgcg ccgtctgcgg aagcgagtcg cgcgtggcga cggacgcgaa gccccggagc
    11341 gtttagtttt actgtccgcg agaaataccg aaatgtccct ttttttagct gaaccagtgt
    11401 cgaagacgcc ggaagagtta tggagagagt acggcgcgct ccagagtgat tttgttgacg
    11461 cgtggcactg gcgcacgcag tcgaacatca gcgtcctcta cgaactcgac gccttcgact
    11521 ggacggtgct catcctctat ttcagcattc tcttcgtgct cgccatctac ggcgcgtatc
    11581 gcatcaagca ggtcgcggac ttctggcgtt atcgccacat tcagcccgcg ccgaaagcgc
    11641 gcttcgccga aagcgacttg cccgtcatca ccgtccagct cccgctcttc aacgagatgt
    11701 acgtcgtcga aagactcctg cgcgccgtga cgcagttcga ctacccgcgc gagcgtctgg
    11761 agattcaagt cctcgatgat tcgacggacg agacggtcgc cctcgcgcgt cgcaccgtcg
    11821 aagcgtatcg cgccgaaggc ttcgacatcc attacatcca ccgcagcgac cgcaccggct
    11881 tcaaagcggg cgcgttggcc aacgggacgc agaccgcgaa gggcgaactg ctcgccatct
    11941 tcgacgccga cttcgtgccc aagcccgact gcctgcgtaa gctcgtagac tatttcacag
    12001 acccgctcgt cggctgcgcg cagatgcgct ggtcgcacat caacggcgcg tacaacctcc
    12061 tgacgcgcct gcaaaccatc atgctcgacg gccatttcgt catcgagcag accgtgcgca
    12121 atcgcacggg cggcttcttc aacttcaacg gcacggcagg catctggcgt cgccgcgcga
    12181 ttgaactctc cggcggctgg cagcacgaca ctttgacgga agacacagac ctcagtttcc
    12241 gcgcgcagtt gatgggctgg cgtttcgtct acctgctcga cgaagacgcg ccttcggaaa
    12301 ttccggttga gatcaacgcc ttcaaggcgc agcagaggcg ttgggcgaag ggcgtgatgc
    12361 aggtcggcat caaactctat ccgcgcatct ggcgcgcgaa tctccctctg cgtgtgcgtc
    12421 tggaaatgtt ctttcgcctg acgggaaata tcagttaccc tctgatgatc gtggcgagct
    12481 tcctacagtt tcccctgctg ctggtgcgct acaatcaggg cttctatcac ctgatggctt
    12541 tcgacgtgcc gctcctcttt ttctcgaccg tctcggtcat tctcttctac ggttcggcca
    12601 tctggtatct ggataaagcg acggggcgcg ggcgtcgcct gctgcacctg ccgctcgtga
    12661 tggcggtcgg catcgggctg gcgttctcga acgcgcgcgc ggttatcgaa gcgttgatgg
    12721 gcgtcaagtc ggagtttgtg cgcacgccga aatatcaggt ggaggcgact tcggacgaga
    12781 cgtggaaggc gaagaagtac aaacgtaagc gcgggctgtt gcctctgctg gagttgagtt
    12841 tcgccgccta cttcttctgc gccgtctggt acgccgcgca catgagcatg tggggcacga
    12901 tcccttttct cttcctcttc ttcttcggct acggctacat cggcctcatg agcatcctcc
    12961 agacggcggg cggcaaacgc ttccaactat ggcgacgccc gctcgccatc ggacgcaggg
    13021 catagagtca gcgtcatgaa atttctataa cagcagggca agctgttggg agagatgaag
    13081 aagcaacgcc cgttagttta tctgactgac gggcgttgct tcttttagca tgtggaagga
    13141 gtgacagcag ttgctttatt cggcacacaa ccggaggcac gcttgccccc gaaagttttt
    13201 tacccggcag ttagtttcct tgaccgcctc gcgcccctca tttaatatct cgcttccgac
    13261 gagccacggg ttttcg
//
LOCUS       DASWNS010000418        10687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_353658, whole genome shotgun sequence.
ACCESSION   DASWNS010000418 DASWNS010000000
VERSION     DASWNS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353658"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="VGB76_14450"
     CDS             complement(<1..1756)
                     /locus_tag="VGB76_14450"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEY0100147.1"
                     /translation="MRIKALPVFASALLLLFCCPLAVLAVQSAPAPAATPTSSAPVDA
                     KKNASNNTSNNTSNNTDANTSAPADNSGTKPLSTVGSSPGAVTPERGTTPVSEAAVPV
                     MVKRGAVALPPEKAAPVRIPRTERPPVIDGRMDEELWRRAAVLKDFYQTNPGDNIAPS
                     FATEVYLTYDARMLYIAFHAHDEPGKVRATVAKRDTVLDTEDSVRVLLDTFNDRRKAY
                     VLVFNPLGVQQDGIRTEGQGVDFSFDLVMESKGALTPDGYVVEAAIPFKSLKYESGKG
                     KLWGIQVFRQIQRFNNETNSWMPISRDINGLLNQAGHITGLEDISTERTIDLIPSLTL
                     SEEGRRVRGLPPGVPAPFGFVDQGRFVNQPFKLDPGFTGKFAISPTMTLDVALNPDFA
                     QVEADQLVVTTNQRFPIFFPERRPFFLEGIEIFQTQLGVVNTRAIVDPDIALKLTGRR
                     GANTYGVLVASDNAPGNFSEDDRTRAIQAEARRTPGEPQGSIRFLDKNAFIGVARVNR
                     NVGQENTIGLVATTYQFKELHNQTGGLDGRFKLDPQTTVSFSLIGTTSHREFPNPDLD
                     ANQLRVGRGFGYTAAYSHF"
     gene            complement(1953..5282)
                     /locus_tag="VGB76_14455"
     CDS             complement(1953..5282)
                     /locus_tag="VGB76_14455"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEY0100148.1"
                     /translation="MRERAVRRSDCFVTTSPLLRPSTQKTPANVAAARLLLPARARRR
                     APARLLRLALSKSLACVMLVALLSPLGRAQEVAKFTGKSKTKTASANETSKTATTASA
                     DARTRRIASLRTRETTEGARVLLTSDAELNNYKSYQSGGRFFVRIPQADAGEAATLAA
                     NLHGRGFEDAQVERSGADVLLSFKLAVGATAEVRQSFNRLEVRFTAQEATPQKKDSNS
                     GGAKDATPAPPLNAAPSPTPTPPSSTPSDAPIITPATTTGSDSSNTTDTVERAKVAAG
                     KGRSIALPPEKANPVVVPRFDTPPVIDGKLDDEIWKRARVLKDFYQTEPGDNIAPSQP
                     TEAFIGYDAKFLYIAFHAYDEPSKIRANVAKRDAIFDDDYVGFFLDTFNDQRRAFEMD
                     FNPLGIQADAIFTENNGEDFSFDLVMESKGMITSDGYTVEVAIPFKSLRYEAGKGKLW
                     GAHFFRRIKRFNNEFNSWMPTSRDVQGYLVQAGKLTGLENISTERTMEIVPSITLSET
                     GKRVRGFPPPADVRATSAAVPDRLLNKPVEFDFGVTTKFGLTPTVTLDFAYNPDFAQV
                     EADQTVVTANQRFPIFFQEKRPFFLEGKEIFDTASTVVHTRAIVDPDYAVKLTGKRGR
                     NTFGLILASDNAPGNFSEEEREEKRFEVLGLRAPQAAGEIDADYFARLNRAAFVESRF
                     NRFVDKNAAIGVLRLKRDVGRESSLGLFATSYNFTEQHNQVIGLDGRFKLDPKTFTTF
                     EIIGTNSKNFFRNLDTDEETYRSRNGFAYNYLLDYTGRYFGYALSANGRTQDYRADVG
                     FTRRTNTNNVNFGWRLSTEPNPKAILTSFRFQNFTNTNFDWQARHQGWSNGQNFNFTF
                     AHQTFVQIGTNFGYERILEEEFGTRRAPGRRGAFAGDDNERQTKHKTIFIYAERQFYK
                     QLYAYFFTGTQRGIFDYDFGAGPRFPRVSPVAIAAREASAAGLCPETTPRKQLPVVCL
                     AQQDPGSGNTFDVEAGVTVQPTSALRSSLDYRKASLKRNDTGLTAFNDNIFTLRTTYQ
                     FTRFLFARGRVDYSTLATSVRGQFLFGWTPNPGTSFYVGYNDDLQRNGFNPFTRQLEP
                     GLRRNGRTFFIKASYLFRRSF"
     gene            5542..5703
                     /locus_tag="VGB76_14460"
     CDS             5542..5703
                     /locus_tag="VGB76_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100149.1"
                     /translation="MLEKSNAPEGEETALKLGNRQCEKSEAEELRTVAGEEEATGRQS
                     GGSSNKESL"
     gene            complement(5878..7833)
                     /locus_tag="VGB76_14465"
     CDS             complement(5878..7833)
                     /locus_tag="VGB76_14465"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY0100150.1"
                     /translation="MSRRVVNRAALLLGVLLLASAGAFAQEEGSARGNELREEFHRSY
                     PLSAQGRVTLKNISGAVRITAWDRAEVRVDAIKRAPTRERLDEVKIVVEASGDSVNVE
                     TRYPENEFNYDGGDKRRNRSDHASVDYVLSVPRGARLESIEVISGQLDIENVTGGVKA
                     SCISGRFTARNLAGDARLSNVSGSLEATFERLDDASTITLSNVSGQLIVNLPSDTNGT
                     IKANTLSGQIANNLGLPVRRGEYVGNDLSGQIGRGGARIRLNNVSGQIRIQRADGRNA
                     APVTNLLPDAGRSVRDEGASQEEIQRRVAREIESAMRETDRSVEHSNREVERANRALE
                     RANRTRPTRDAAQRNERAQRQADRALRDMERNQAQIARDVERAAREMERVMDEHGRSV
                     AEHGESSYRLVERESKSFNVSGMPRVVLKTFDGSVNIQPSNEATVRFSAVKRARDEQA
                     MRGIRLRAEQTGNTIQIIAEFDKTVARKDEYGTGAAVALDVWIPRNANVQVQSGDGRL
                     RLSGIDGEINLLTGDGSVDVTESRGRLRVETGDGRVRVAGFDGEATVQTGDGRISLDG
                     RFSKLSASTGDGAITLALPTDANARIETDSERVINDTSAVAEEAEAETPERRVRHWRI
                     GSGAGSQITLRTGDGQIFLRRTGDERE"
     gene            complement(7945..8850)
                     /locus_tag="VGB76_14470"
     CDS             complement(7945..8850)
                     /locus_tag="VGB76_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100151.1"
                     /translation="MLRFRWMQAFLFVVIAVLTAPQISSQFAGLKATVADSFAASAQE
                     NQDKDKHKDHKGEKQKNEHRSYDDSEFTEKDEIHQNYQLTPGATVRVAGINGQVDVET
                     TGGSTAEVHIVRSARSRADLEHRKVIIEHTGGSLVVRGENEGERRYARGEQPQVRQRV
                     TLRLPRQIDLTASGINGKTTVGEVDGPVKISGINGRVEVAQAVGYSDLSGINGTVVVT
                     IAQLGERGIRVNGINGAVELRFTEALNADLRVTGINGSVNADMPNVSVLGTVSRTNFN
                     ARIGSGGAPINVSGVNGRVRLSPRS"
     gene            complement(9005..10021)
                     /locus_tag="VGB76_14475"
     CDS             complement(9005..10021)
                     /locus_tag="VGB76_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100152.1"
                     /translation="MMRRKLGILILAGVTVLAGTQEAMRQFDGLRSSVKDLTRASLWS
                     GLIVYAQPVSDGKLPAPQIYYLMPQQTSPASPQEPVLVNNNNADPNHVAAKSETRNNH
                     GGEELAAAVEASAEEALPQALLPLREFEDAAKSELVLNELASHRVAPHVAPAPKLERA
                     RVYEEVARHEAGARKFKQHADATARVFMKEFDAVKLGEEIAQLVAAREQLENSKLKSF
                     ADAENMERRLELRVLRRAARPVERPERHEPRIKVVAPHAYRRAITALPEISTIGCEKV
                     KTRVKSPAAVEAIVAAAGVASSVRIAEEPSVAVESSSPGVMPSPLVTSTSWALGCDTE
                     PEHR"
     gene            complement(10430..>10687)
                     /locus_tag="VGB76_14480"
     CDS             complement(10430..>10687)
                     /locus_tag="VGB76_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100153.1"
                     /translation="LPADASFQLNAKVHHSGEIITEFPIRQVPVMMSDNPRNSAPVGG
                     ALLQDGRLTGVTGKSDRRAATLILSSFSGTVHLQQQGHTPQ"
BASE COUNT         1821 a         3168 c         3462 g         2236 t
ORIGIN      
        1 cgaagtgcga ataggccgcc gtgtagccga agccccggcc cacgcgcagt tggttggcgt
       61 cgaggtcggg attcggaaac tctctgtgcg aggtggtgcc gatgagggag aaactaacgg
      121 tcgtctgagg gtcgagcttg aagcgaccgt cgaggccgcc cgtctgattg tgcagttcct
      181 tgaactggta ggtggtggcg accagaccga tggtgttctc ctgcccgacg ttgcgattga
      241 cgcgggcgac gccgataaaa gcgttcttat cgaggaagcg gatgctgccc tgcggttcgc
      301 cgggcgtgcg tcgcgcctcg gcttgaatgg cgcgcgtgcg atcatcttcg gagaagttgc
      361 cgggtgcgtt gtcgctcgcc acgaggacgc cgtaagtgtt tgcgccgcgc ctgcccgtca
      421 gtttgagcgc gatgtccgga tcgacgatgg cacgcgtgtt gacgacgccg agttgggtct
      481 gaaaaatctc gatgccttcg aggaagaagg ggcggcgttc ggggaagaag atggggaagc
      541 gttggttggt cgtcacgacg agttgatccg cttcaacctg cgcgaagtcg ggattcagag
      601 ccacgtcgag cgtcatcgtc ggcgagatgg cgaacttccc cgtgaagccg gggtcgagtt
      661 tgaagggttg attgacgaag cggccttgat ccacgaagcc gaagggggcg ggcacgccgg
      721 gcgggaggcc gcgcacgcgc ctgccttctt cggacagggt gaggctcgga atgagatcga
      781 tggtgcgctc ggtggagatg tcttcgagtc cggtgatgtg tccggcctga ttcaagaggc
      841 cgttgatgtc gcgcgagatc ggcatccacg agttggtttc gttgttgaac ctttgaatct
      901 ggcgaaagac ctgtatgccc cagagtttgc ccttgcccga ttcatacttg agcgacttaa
      961 acgggatcgc cgcttcgacc acgtagccgt ccggcgtgag cgcgcccttc gattccatca
     1021 cgaggtcgaa actgaaatcc acgccctgcc cttcggtgcg gatgccgtcc tgctgcacgc
     1081 cgagcgggtt gaagacgagg acgtaagcct tgcgccggtc gttgaaggtg tcgagcagga
     1141 cgcgcacgct gtcttccgtg tcgagcacgg tgtcgcgttt ggcgaccgtg gcgcgcacct
     1201 tgcccggctc gtcgtgcgca tggaaagcga tgtagagcat gcgcgcgtcg taagtcagat
     1261 aaacttcggt ggcgaacgag ggcgcgatgt tgtcgccggg gttggtctgg tagaaatctt
     1321 tgagcacggc ggcgcgacgc cagagttctt cgtccatcct gccgtctatc acgggcggac
     1381 gctccgtgcg cgggatgcgg acgggcgcgg ccttctccgg cggcagcgcg accgcgcctc
     1441 gcttgaccat gacgggcacg gcggcctcgg atacgggagt cgtgccgcgt tcgggcgtga
     1501 ccgcgccggg cgatgagccg acggtggaga gcggcttcgt accggagttg tcggcgggcg
     1561 cagacgtgtt cgcatccgtg ttgttggagg tgttgttgga ggtgttgttg gaggcgttct
     1621 ttttcgcatc cacgggcgcg ctcgaagtgg gcgtcgcggc tggcgcaggc gcggattgca
     1681 cggcgaggac ggcgagcggg cagcagaaca acagcagcag ggcggacgcg aaaaccggca
     1741 gagcttttat tctcatagct tccttcgagt cttacagatt tcgagtgaaa ggctcttacg
     1801 gtttttcact ctattcaagc catctcagct tgtaagagtg cgtcgcgcgg agaaagtttc
     1861 aaaaaacagc gacgagtgaa gggtgacaag cgacgagtta aaactaaaac ctgtcttgac
     1921 tcctgctcgt cacccgtcac ttgtcgctgt ccttagaagc tgcggcggaa caggtacgac
     1981 gccttgatga agaaggtgcg accgttgcgg cgcaggccgg gttcgagttg ccgggtgaac
     2041 gggttgaagc cgttgcgttg cagatcgtcg ttgtagccga cgtagaagga ggtgccggga
     2101 ttcggcgtcc agccgaagag gaactggccg cgcacgctcg tcgccagcgt gctgtagtcg
     2161 acgcgcccgc gcgcgaagag aaagcgcgtg aactggtagg tggtgcgcag cgtgaagatg
     2221 ttgtcgttga aggctgtgag tccggtgtcg tttcgcttca agctcgcttt acggtagtcg
     2281 agtgaggagc gcagcgcgct cgtcggctgc accgtgacgc ccgcttccac gtcgaaggtg
     2341 ttgccggagc cggggtcttg ctgggcgaga cagacgacgg ggagttgctt gcgcggagtc
     2401 gtctcaggac agagtcccgc cgcactcgct tcgcgcgcgg cgattgctac agggctgacg
     2461 cgcgggaagc gtggacctgc gccgaaatcg taatcgaaga tgccgcgctg cgtgcccgta
     2521 aagaagtagg cgtagagttg tttgtagaac tgccgctcgg cgtagatgaa gatcgtcttg
     2581 tgcttcgtct gccgttcgtt gtcgtctccg gcgaacgcgc cgcgcctgcc cggcgcgcgc
     2641 cgcgtgccga actcttcttc gagaatgcgc tcgtaaccga aattcgtgcc gatctgcacg
     2701 aaggtctggt gcgcgaaagt gaagttgaag ttctggccgt tgctccaacc ctggtgtcgc
     2761 gcctgccagt cgaaattcgt gttcgtgaag ttctggaagc ggaacgaagt gaggatggct
     2821 ttcgggttcg gctcggtgct cagacgccag ccgaagttga cgttgttggt gttggtgcgg
     2881 cgcgtgaagc ccacgtcggc gcggtagtcc tgcgtgcgcc cgttcgcgct cagcgcgtag
     2941 ccgaagtaac gcccggtgta atcgagcagg tagttgtagg cgaagccgtt gcggctgcga
     3001 taagtctcct cgtcggtgtc gaggttgcgg aagaagttct tcgagttcgt gccgatgatc
     3061 tcgaaggtcg tgaaagtttt cggatcgagt ttgaagcgac cgtccaagcc tatgacttga
     3121 ttgtgctgct cggtgaaatt gtagctcgtg gcaaacaggc cgaggctcga ttcgcgccct
     3181 acgtcgcgct tgagacgcag cacgccgatg gccgcgttct tgtccacgaa gcggttgaaa
     3241 cgcgactcga cgaaggcggc gcgattcagg cgcgcaaagt aatccgcatc gatctccccg
     3301 gctgcctgcg gcgcgcgcag gccgagcacc tcgaaacgct tctcctcgcg ttcctcttcg
     3361 ctgaagttgc cgggggcgtt gtccgaagcg aggatgaggc cgaaggtgtt gcgcccgcgc
     3421 ttgcccgtga gtttgacggc gtaatcggga tcgacgatgg cgcgcgtgtg gacgaccgtg
     3481 ctcgcggtgt cgaaaatctc tttgccttcg aggaagaagg gacgcttctc ttgaaagaag
     3541 atggggaagc gttggttggc cgtgacgacc gtttggtccg cttcgacctg cgcgaagtca
     3601 ggattgtagg cgaagtcgag cgtcacggtc ggcgtcaggc cgaacttggt ggtgacgccg
     3661 aagtcgaatt ccacgggctt gttgagcagg cgatcaggca cggcggcaga tgtcgcgcgc
     3721 acgtcggcgg gcggcggaaa gccgcgcacg cgcttgcccg tctccgagag cgtgatgctc
     3781 ggcacgatct ccatcgtgcg ttcggtggag atgttttcga ggccggtcag ttttccggct
     3841 tgaacgagat agccctgcac gtcgcgcgag gtgggcatcc atgaattgaa ctcgttattg
     3901 aagcgtttga tgcgacggaa gaagtgcgcg ccccagagtt tgcccttgcc cgcttcgtaa
     3961 cgtagcgact tgaaagggat cgccacttcc accgtgtagc cgtcggaagt gatcatgccc
     4021 ttcgattcca tcacgaggtc gaagctgaaa tcctcgccgt tgttttcggt gaagatggcg
     4081 tcggcctgta tgccgagcgg gttgaaatcc atctcgaagg cgcggcgttg atcgttgaag
     4141 gtgtcgagaa agaagcctac gtaatcgtcg tcgaagatgg cgtcgcgctt ggcgacgttg
     4201 gcgcgaatct tggacggctc gtcgtaggcg tgaaaggcga tgtagagaaa ctttgcatcg
     4261 tagccgatga aggcttcggt cggctgcgag ggcgcgatgt tgtcgcccgg ttcggtctgg
     4321 taaaaatctt tcaggacgcg tgcgcgtttc cagatttcgt cgtcgagttt tccgtcgatg
     4381 acgggcggcg tatcgaagcg cgggacgaca accgggttcg ccttctccgg cggcagggca
     4441 atcgaacgtc ctttgcccgc ggcgactttc gcgcgttcga ccgtatcggt cgtgttcgag
     4501 ctatcgctcc cggtcgtggt ggccggggtg atgatgggcg cgtcgctcgg cgtgctggat
     4561 gggggagtcg gggtcgggct gggcgcggcg ttgagaggcg gcgccggagt cgcatctttc
     4621 gcgccgccgc tgttcgaatc ttttttctgc ggcgtggcct cctgcgccgt gaagcgcact
     4681 tcgagccggt tgaaactctg cctgacctcc gcggttgcgc ccacggccaa cttgaaagag
     4741 agcagaacgt ctgcgccgct ccgctcgacc tgcgcgtctt caaagccgcg cccgtgcaag
     4801 ttcgcggcga gcgttgccgc ttcgcccgcg tccgcctgcg ggatacgcac gaagaagcga
     4861 ccgccgctct gatagctctt gtagttgttc aactctgcat ccgaagtcag gaggacgcgc
     4921 gcaccttccg tcgtctcgcg cgtgcgaagc gaggcgatgc gacgcgtgcg cgcgtcggca
     4981 ctcgccgtcg tcgccgtctt cgaagtttcg tttgcggatg ccgttttagt cttcgacttg
     5041 cccgtgaact tcgccacctc ctgcgcgcgt ccgagcggcg acagcagggc gacgagcatg
     5101 acacaggcca gactcttgga gagcgcaagg cgcaataacc gtgcgggggc acgacgacgc
     5161 gcgcgcgccg gaaggagcag gcgtgcggcg gcgacgttgg ctggcgtctt ctgcgtcgaa
     5221 gggcgaagga gtggcgatgt cgtcacgaaa cagtcggagc ggcggacggc tctctctctc
     5281 atgtgtattg acctcatgtt ctaaaggcgc gcaaagctcc cgcgctttac gctccgctag
     5341 ttgcgacgaa gcgggtaaga aatcctgtcg aagtatggcg cgagcatggc tgcggaagtc
     5401 tgtctcaagt taaccggcgg aagactggag ccgttacagc gtgtggaacg agcgtgaacg
     5461 acgatgcgcc tgatgcgcgt tcggtcgggc ggcggcgagg ggcgcgaggc ttgcttcgat
     5521 tgacagccgg tagatgccgg ggtgttggag aagtcgaacg cgccggaggg ggaagagacg
     5581 gcgttgaagt tggggaacag gcagtgcgaa aagtcggaag cggaagaact gcgaaccgtc
     5641 gccggagagg aggaggcgac gggcaggcag agcggtggca gttcaaacaa ggagagcctc
     5701 tgagtcataa cgtctggtct taaactttta gcgattcgga aaccggggct tgctcgccgc
     5761 atcgggagaa cgcggactca aggccatttg gctattagac gttcgccccc cgcaagatgt
     5821 ttcatctaat taaaagaaga tttttccgct ttccgctttc cgaaaattcg taaactgcta
     5881 ttcgcgctcg tcgcccgtgc ggcgcaaaaa gatttggccg tcgcccgtgc gcagcgtgat
     5941 ctggctgccc gcgccgctgc cgatgcgcca atgcctgacg cggcgttcgg gcgtctccgc
     6001 ctcggcttct tcggcgaccg ccgacgtatc gttgatgacg cgttcggaat cggtttcgat
     6061 gcgcgcgttg gcatcggtgg gcagagcgag cgtgatcgcg ccgtcgcccg tgctcgcaga
     6121 gagtttgctg aaacgaccgt cgagcgagat gcgcccgtcg ccggtctgca ccgtcgcctc
     6181 gccgtcgaac ccggcgacgc gcacgcgccc gtcgcccgtc tccacgcgca gccgtccacg
     6241 gctctccgtt acgtcaaccg agccgtcgcc cgtcagcaag ttgatctcgc cgtcgatgcc
     6301 gctcaggcgt aaacgcccgt cgcccgattg cacctgcacg ttcgcgttgc gcggaatcca
     6361 cacgtcgagc gcgacggctg cgcccgtgcc gtactcgtct ttgcgcgcga ccgtcttatc
     6421 aaactccgcg atgatctgga tggtgttgcc cgtctgctcc gcgcgcaggc ggatgccgcg
     6481 catggcctgt tcgtcgcgcg cacgtttgac ggcggagaaa cgcacggtcg cttcgttgga
     6541 gggctgaatg ttgacggagc cgtcgaaggt cttgagcacg acgcgcggca tgcccgacac
     6601 gttgaaagat ttcgactcgc gttcgaccag acgatacgat gactcgccat gctccgcgac
     6661 actgcgcccg tgttcatcca taactcgctc catctcccgc gccgcgcgct ccacatcgcg
     6721 cgcgatctgc gcctgattgc gttccatatc gcgcagggca cgatccgctt gacgctgcgc
     6781 gcgttcgttt ctttgcgccg catcgcgcgt ggggcgcgtg cggttcgcgc gttcgagtgc
     6841 gcggttcgcg cgttccactt cacggttcga atgctcgacg ctgcgatccg tctcccgcat
     6901 cgcgctctcg atctcgcgtg cgacccgtct ctgtatctcc tcctgcgacg cgccttcgtc
     6961 gcggacgctg cgcccggcat cgggcaggag attcgtgacg ggcgcggcgt tgcgcccgtc
     7021 ggcgcgttga atcctgatct ggccgctcac gttgttgagc ctgatgcgcg cgccgccgcg
     7081 cccaatctga cccgacaggt cgttgcccac atactcgccg cgcctgacgg gcaggccgag
     7141 gttattagcg atctggccgg agagcgtgtt ggctttgatc gtgccgttgg tgtcggaggg
     7201 aaggttgacg atgagttgac cgctcacgtt gctgagcgtg atcgtcgaag catcgtcgag
     7261 ccgttcgaag gtcgcttcga gcgagccgct gacgttcgac aaacgcgcgt cgcccgcgag
     7321 attgcgcgcc gtgaagcgcc cgctgatgca cgaagccttg acgccgcccg tcacgttctc
     7381 gatgtcgagt tggccgctga tcacttcgat tgattcgagg cgcgcgccgc gcggcacgct
     7441 caacacgtaa tccacgctcg cgtgatcgct gcgattgcgc cgcttgtcgc cgccgtcgta
     7501 gttgaactcg ttctccgggt agcgcgtctc gacgtttacg ctgtccccgc tcgcttcgac
     7561 cacgatcttc acttcgtcca gacgttcgcg cgtcggggcg cgcttgatgg catccacacg
     7621 cacctcagcg cgatcccagg ccgtgatgcg caccgcgccg ctgatgttct tcagcgtcac
     7681 gcgcccctgc gccgagagcg ggtagcttcg atgaaactct tcgcgcaact cattgccgcg
     7741 cgcgcttccc tcttcctgcg caaacgctcc cgcgctcgcc aacaacaaca cgcccagcag
     7801 caacgccgca cgattcacaa ctcttctgct catcactttc acctctcaaa atcagtgaca
     7861 ggtgacgggt gacaagtgac aggattgaag ccaagcatgc tgctttaact tgtcacccgt
     7921 cacctgtcac ccgtcactgt tttctcagct tcggggcgag agacgcacgc gaccgttgac
     7981 gcccgagacg ttgatcggcg cgccgcccga accgatgcgc gcgttgaaat tggtgcggct
     8041 cacagtgccg agcacggaga cgttcggcat gtcggcgttg accgagccgt tgatgcccgt
     8101 cacacggaga tcggcgttga gcgcctcggt gaagcgcagt tcgaccgcgc cgttgatgcc
     8161 gttgacgcgg atgccgcgct caccgagttg cgcaatcgtc acgacgaccg tgccgttgat
     8221 gccggagagg tccgagtagc cgacggcctg cgccacttcg accctgccgt tgatgccgct
     8281 gatcttcacc ggaccatcaa cttcgccgac ggtcgttttg ccgttgatgc cgctcgccgt
     8341 gagatcgatc tggcgcggca ggcgcagcgt cacgcgctgc cggacttgcg gctgctcgcc
     8401 gcgcgcatac cgacgctcgc cttcattttc gccgcgcacg acgagcgacc cgccggtgtg
     8461 ctcgatgatc actttgcggt gttcgaggtc ggcgcggctg cgtgccgaac gcacgatgtg
     8521 aacttcggcg gtgctgccgc cggtcgtctc cacgtctacc tgcccgttga tgcctgcgac
     8581 gcgcacggtc gcgcccggcg tcaattgata gttctgatga atctcatctt tttcggtgaa
     8641 ctcgctgtcg tcgtaactgc gatgttcgtt cttctgtttc tcgcccttgt gatctttatg
     8701 tttatcctta tcctgattct cctgcgcgga ggcggcgaaa ctgtcggcga cggtcgcctt
     8761 caagcccgcg aactggctgc tgatctgcgg cgcggtcaac acggcgatga cgacgaaaag
     8821 gaaggcttgc atccatctga atctcaacat gtttttttct cctgttggcg gctaaatcaa
     8881 ttcgtaactt actgcgcggt ttcaggcaca cacctgccct tgagcggaaa ctcgctgcct
     8941 ccgccaatta atgatccgac ttgtacggga aactttttac cggacgcgaa cggcgcggcg
     9001 catcctacct gtgctcaggc tccgtgtcgc agccgagcgc ccaactcgtg ctcgtcacga
     9061 gcggcgaggg catcacgccg ggcgacgaag attcgacggc aaccgacggc tcttccgcaa
     9121 ttctcacgct cgacgccacg cctgcggcgg cgacaatcgc ttcgaccgca gcgggagact
     9181 tcactctcgt cttcaccttc tcgcaaccga tggtcgaaat ttccggcagc gcagtgatgg
     9241 cgcgcctgta agcatgggga gcgacaacct tgatgcgagg ttcgtggcgt tccgggcgtt
     9301 ccacgggacg agcggcacga cgcaggactc tgagttccag acgccgctcc atgttctccg
     9361 cgtcggcaaa ggatttgagc ttggagtttt ccaactgttc ccgcgccgcc acgagttgcg
     9421 cgatctcctc gccgagtttg acggcgtcaa actccttcat gaacactctg gcggtcgcgt
     9481 ccgcgtgctg tttgaacttg cgtgcgcccg cttcgtgccg cgccacctct tcatagacgc
     9541 gcgcccgctc caacttcggc gcgggagcga cgtgcggcgc gaccctgtgc gaagccaact
     9601 cgttcaagac caactcggac ttcgccgcgt cttcaaattc gcgcaaaggc aggagcgcct
     9661 gcggcagagc ctcttccgcg ctcgcctcta cggctgcggc caactcttcc ccgccgtgat
     9721 tgttcctcgt ctcggatttc gcggcgacgt ggttggggtc ggcgttgttg ttgttgacga
     9781 gcacgggttc ctgcggtgag gcgggcgagg tctgctgcgg catcaggtag tagatctgcg
     9841 gcgcgggcag tttgccgtcg ctgacgggct gcgcatagac gatgaggccg ctccacaggc
     9901 tcgcacgcgt caaatctttc acagacgacc gcaggccgtc gaactgtcgc atggcttcct
     9961 gcgttccggc caacactgtg acaccggcaa gaattaagat tcccaatttt cgtcgcatca
    10021 tctcactacc tcccaacctc tgcatgcgcg gcgttagtgc tgctcgcact catcttttgt
    10081 cccatttccg caaacgtccg ttcgcgttct cgctactgcg cggatcgcgt ctcatgcagg
    10141 tagacgcggc cagcgaccga aacgtttcaa accgccgctt tttcaaacca actaaccact
    10201 tatactgtga aaacaccgcc accgttgatt ttgtggcaag atttttttcg cgtaggattt
    10261 tcaattttcg acttgcaggc cagcggttcc gtgcggcggc gcggagtcaa tctcccgatc
    10321 attctgcaaa tcacagacca taactaataa acgcctgccg cacactaact attaccgcga
    10381 cgagcaacga aaagtttccg gcgaaaaacc gaaaactctg aagcgaagac tattgcggcg
    10441 tgtgaccttg ctgctgcaag tgcaccgtcc cgctgaaaga ggacaaaatg agcgtcgccg
    10501 cgcgtctatc gctcttgccc gtgacgcccg tgaggcgtcc atcctgaagg agcgcgccgc
    10561 ccacgggagc ggaatttcgc gggttatcgc tcatcatcac gggcacctgc cggatcggaa
    10621 actccgtgat gatctcgcct gagtgatgaa ctttggcgtt gagttgaaac gaagcgtctg
    10681 cgggcag
//
LOCUS       DASWNS010000419        11150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_170595, whole genome shotgun sequence.
ACCESSION   DASWNS010000419 DASWNS010000000
VERSION     DASWNS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170595"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VGB76_14485"
     CDS             <1..225
                     /locus_tag="VGB76_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100154.1"
                     /translation="PPHVEYALTERGRLLLPLLESLRQLGLSLGCNECEDRHAALGDY
                     CDACPRKDRPREHTPTPARQRERDDSIVLL"
     gene            complement(319..1410)
                     /gene="trxB"
                     /locus_tag="VGB76_14490"
     CDS             complement(319..1410)
                     /gene="trxB"
                     /locus_tag="VGB76_14490"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY0100155.1"
                     /translation="MTQETLHKEVVIIGSGPAGLTAAIYAARADLKPLVIEGPQPGGQ
                     LTITTEVENYPGFAEGIQGPELMEQFRAQAARFGTEFLVTWIDRVDLSARPFTLHTAD
                     QTIKAETLIIASGASAKWLGIPGEAPAPHGLGGLGVSACATCDGFFFKGKKLVVVGGG
                     DTAMEEATFLTRYASSVTVVHRRDSLRASKIMQDKAQKNEKIDFIWNTDVREILGSPE
                     SGVTGVRLYNNQTHEETTLDCEGVFIAIGHQPNTAMFKGWLEMDEVGYLKTNGRTMAT
                     NIPGVFACGDAQDSFYRQAITAAGTGCMAAIDAERFLDNLPVPLLTGEEVTIEGEIVT
                     PDHEAIIMPDGEIISNHVESESEGASAGD"
     gene            1567..2538
                     /locus_tag="VGB76_14495"
     CDS             1567..2538
                     /locus_tag="VGB76_14495"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0100156.1"
                     /translation="MSADTDEALFVAPEAAREDILDSPGSNAIASSHIDTIAVIGAGT
                     MGHGIAQVAALAGYRVVLRDVEREFLMRGLRAIEENLEKGIRRGKTTEAERDLVLQRI
                     RGATSLSETAHADLFIEAVPERMELKQETLSAVEQIAERDFIFATNTSSLSITEIASA
                     AAHPERVVGMHFFNPVHIMRLVEIVVGSETSDETVETARAVGQRMKKEPITVRDVPGF
                     ASSRLGVALGLEAMRMLEQGVASAKDIDTAMELGYNHPMGPLRLTDLVGLDVRLSIAE
                     YLHRALGSETFRPPEILRRMVAEGRLGKKSGEGFYDWNEEEQVSGRR"
     gene            2612..2980
                     /locus_tag="VGB76_14500"
     CDS             2612..2980
                     /locus_tag="VGB76_14500"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0100157.1"
                     /translation="MTTPTNEERRFALRMALRLPIVVSGRAEDGAAWSEPTETDDIST
                     TGALFHLNQKVTRGEQLYLRAHRPDGAPIEVTARVVRIASAIYGTARIGVAILEPTEN
                     WLRLFVSWVADDQVAQDSDG"
     gene            3091..6015
                     /locus_tag="VGB76_14505"
     CDS             3091..6015
                     /locus_tag="VGB76_14505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0100158.1"
                     /translation="MLKRIFFALALFAALCCSAVHAQDCAPPPLVANAKSANLFTPEQ
                     EMIFGELTLQNMSGEIRFLRDEALQAYINAIGGKLAKHLPPIGLRFQFHLVDLPETNA
                     FNIPGGHVFLSRKLVAFVNNEDELAGVIAHELGHAVVRHGATDMSDALRKILNISALG
                     DRKDITEKYNLLIERARTKTFSRKRDHENEQQLEADRVGLFAMVAAGYDPAAFSAFFD
                     RLTESEGKTGGWFSDLFGKTRPEHKRLREMIRATEKLAPSCRDGRTAKTTGDFLKWQA
                     DVVSFRETGRKEELPGLLWKKELAPKLRSDVSHFAFSQDGKFLLAQDDFAVTVIDREP
                     LQVLFQIPVEEANGASFTPDGRFVVFTTENLRYEKWSVAERKPVEVRELVVRRDCWEH
                     KLSPDGNYLACVDTSTTINVLETRTGKRVWEKKEFYPLTAFEFFFWLASSRGEADNKT
                     NFFRIEFSPDSRYVLFSRSDKFRFRISIDGMTAAASENTALALDLTTLQPAEIGGDLK
                     KIASRPYAFLNAGEVLGMPSRKMEEAGVFSFPAGKRLQKFTFAAQEIKRTANPSYVII
                     KPLANARMGVFDLKKGIITSGMNKADGTLWNDLMAFEATNGKILLREVKYNEEKKSFD
                     GKEIGTVEIPVGSLSNLNAAEVSDNFNWLVLSSKTRGGLWNLETGERKLYVRGFKGGI
                     VANDGGGVADFAKLDDTQHSLVLMNALDQTVTPIQELPEKGAQQYGRFVLLRSSLKEK
                     KEEPKSVSAFFGGDESDGSGLRREVRFELKDFIQNKVIWSREFPKAAPEYSFDEFSGR
                     LIFYWRLGSEAGKAKLRESTELQAKADALGNKTDDYLVEVVDAFAQKTVGLLLLETGN
                     GSFSVGAGLSEADWLVLHDSGNRVLVYSIKSGDLRHRFFGSVAAINPKKNQLAVENLP
                     GEVALYDLDSGERQTTFVIGGSAAFVRFNLKGDRFFVLSDAQSAYAFDLNKLAPKTSP
                     AK"
     gene            6110..6895
                     /locus_tag="VGB76_14510"
     CDS             6110..6895
                     /locus_tag="VGB76_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0100159.1"
                     /translation="MSQSYETLLVERRGRVAIITINRPEKRNALNIQTRAEGAAALEE
                     LREDETVRVVVFTGAGDKAFIAGADIAEFAGRTANVQRDVMTGRSLFTAIDTFPKPVI
                     AMINGYCLGGGCELALACDLRIASERASFGQPEINLGIIPGGGGTQRLTHLVGEGKAM
                     ELILTGEIIDAQTAYAYGLVNMVVPAEELEAKTLEIANRIAEKSPIALRMAKEAVKTA
                     SRSTLDEGLRREIDLFALCFSSEDKDEGVKAFMEKRKPEFKGR"
     gene            7093..7168
                     /locus_tag="VGB76_14515"
     tRNA            7093..7168
                     /locus_tag="VGB76_14515"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7126..7128,aa:Ala,seq:ggc)
     gene            complement(7175..8284)
                     /locus_tag="VGB76_14520"
     CDS             complement(7175..8284)
                     /locus_tag="VGB76_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0100160.1"
                     /translation="MPVENRGTPKNPRWRYAFTIRGVRYRAAIPEARTKYEAEQAELE
                     AKKDVFEGRYGRPSGGQDFVQFVEEVYKPWARENRRSWETNIRYTVIVLCEWFKGKTF
                     AQISPLLIEKYKRERRESITNRGTTRKPSSTNRELQLLSSIFTLAIRHKMVATNPCQD
                     VELLAENNKRTRYLLDEEELRLMATLTGERAHLRPFVTVALGTGMRRGDQLNLRWEKV
                     DFQRNVIYVPNSKTGKDYPVPMNEEVRNTLLALRRAPSSVEYVFINPETGKPYAVLKK
                     AFGTACRLAGINGLRWHDLRHTFGTRLAEDGWSESTIAELMGHSNPNTTRRYTHATDW
                     GKQNAVAAASLSGRKARPKYAPKQEQQPFPAAVSS"
     gene            complement(9196..9738)
                     /locus_tag="VGB76_14525"
     CDS             complement(9196..9738)
                     /locus_tag="VGB76_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100161.1"
                     /translation="MSDKENSEIQGNDEQEEFFTRGGFIYVGATLARNGRLQEAREQF
                     QTQVDSLNGCLFLVDEQVSIEGRIPTEVALTYLEVLKDVISDARDDLDDVEQIAGELF
                     EALEREVKSEREAQPSPDPYSLLRGWRACMAGTRPSDWEQIVSHLLIDTDAALASAGA
                     AARKTERRERERSTEGSGGA"
     gene            complement(9821..10402)
                     /locus_tag="VGB76_14530"
     CDS             complement(9821..10402)
                     /locus_tag="VGB76_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100162.1"
                     /translation="MELEQRTASSEKKMRDEYTELLEEQERIARDFEKQRQRYEELKS
                     QRAAVRAQIYVLRERIVAAQGEQCAYCGAHLRSNPAYQLYQELRELELEGFVDEETLT
                     ARWAEEPIPYEERVELQIRLKVPKSRGGLEVMENLVACCIKCGPRKHNKTHQEFMHQL
                     EGERAYAAVARKREIRRTGGDSESPAGHDGPSG"
     gene            complement(10416..>11150)
                     /locus_tag="VGB76_14535"
     CDS             complement(10416..>11150)
                     /locus_tag="VGB76_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100163.1"
                     /translation="SSSQWHETDRRRQREAIRIAAADNYSMIPDAPMKRRKQKRPRSA
                     VDVMKTCQKIILTNLEKLEEMAAQLPANQREELLDGEEPEGYLNGWTRVQTKIESVVN
                     LTLAQVQAGHEVKRGLGNFVLTPLAEEMPAVSDVEPEADPEAAATWERLAERLTTPAV
                     RSVEIELHGAEGPPGQAGQLPEELEEELTLEEEQAAIMQYEGGLRREEADALARGEVV
                     NRRNDHASRRWGRDSEAAAARDGPSG"
BASE COUNT         2176 a         3543 c         3141 g         2290 t
ORIGIN      
        1 cccccgcatg tcgaatacgc tctgaccgaa cgcgggcggc tgttgcttcc gctgctcgaa
       61 agtttgcgtc aactcggact ctcgctcggc tgcaacgaat gcgaagaccg ccacgccgcg
      121 ctcggcgact actgcgacgc ctgcccgcgc aaagacaggc cacgcgaaca cacgcccacc
      181 cccgcacggc aacgcgaacg cgacgattca atcgtgctgt tgtaatcgcc gcgacaattc
      241 ccacgcaaaa acaggcaggc actaaggatt taaatcttcc ttagtgcctg cctctcttag
      301 tgctgatccg aaccgcgcct aatcccctgc ggacgcccct tcggattcgc tttcgacgtg
      361 gtttgagatg atctcgccgt cgggcatgat gatcgcttcg tggtcgggcg tgacgatctc
      421 gccttcgata gtcacctctt cgcccgtcag caggggaacg ggcagattgt cgaggaatcg
      481 ttcggcgtcg atggcggcca tgcagcccgt cccggcggcg gtgatggcct gacggtagaa
      541 ggagtcctgc gcgtcgccgc aggcaaagac gccgggaatg ttcgtcgcca tcgtgcgccc
      601 gttggtcttg agataaccta cttcgtccat ctccagccag cctttgaaca tcgctgtgtt
      661 cggctggtgg ccgatggcga tgaagacgcc ttcacaatcg agggtcgtct cttcgtgcgt
      721 ctggttgttg tagaggcgca cgcccgtcac gcccgactcc ggcgagccga gaatttcgcg
      781 cacgtccgtg ttccagatga agtcgatctt ctcgttcttc tgcgccttgt cttgcatgat
      841 cttcgaggcg cgcagagagt cgcggcgatg cacgacggtg accgaggagg cgtaacgcgt
      901 gagaaaggtc gcttcctcca tcgcggtgtc gccgccgccg acgaccacca gtttcttgcc
      961 cttgaagaag aagccgtcgc aggtggcgca ggcggagacg ccgaggccgc cgaggccgtg
     1021 cggcgcggga gcttcgcccg gaatgccgag ccacttggcc gacgcgcccg aagcgatgat
     1081 gagcgtctcg gctttgatcg tctggtcggc ggtgtggagc gtgaacgggc gcgcggagag
     1141 atcaactctg tcaatccacg tgacgagaaa ctcggtgccg aaacgcgcgg cctgcgcgcg
     1201 aaactgttcc atcagttccg gtccctgaat gccttcggcg aagcccggat agttctccac
     1261 ttcggtcgtg atggtcaact gtccgccggg ctgtggccct tcgatgacga gcggcttgag
     1321 gtctgcgcgc gccgcgtaga tggccgcagt cagtccggcg gggccggagc cgatgatcac
     1381 cacttctttg tgcagagttt cttgtgtcat ctcttttctg gttgcactcc ttaatgcgcc
     1441 gctggagcgg ctgctcgcac gcgcgttttc gcgccccact ctcccgaatt actcacttcg
     1501 catgtagcct tttcgcttgc gcgtatagta taactctctg cgctggatgt agtcgaatca
     1561 gaccttatga gcgcggacac ggacgaagcc ctcttcgtcg cccccgaagc agcgcgcgaa
     1621 gacattttgg actcaccggg aagcaacgca atcgctagtt cgcacataga cacaatcgcc
     1681 gtgatcgggg cgggcacgat gggacacggc atcgcgcagg tcgccgcgct ggccggctac
     1741 cgcgtcgtgc tgcgcgatgt cgaacgcgaa tttctcatgc ggggcttgcg cgccatcgaa
     1801 gagaatctgg aaaagggcat ccggcgcggc aagacgacgg aggccgagcg cgacctcgtg
     1861 ctgcaacgca ttcgcggcgc gacgagcctc tccgagacgg cgcacgcaga cctctttatc
     1921 gaagccgtgc ccgagcgcat ggagttgaag caggagacgc tcagcgcagt cgagcagatc
     1981 gcggagcgcg atttcatctt tgcgacgaac acctcttcgc tctcgatcac ggagatcgca
     2041 agcgcagcgg cacatcccga acgcgtcgtc ggcatgcact tcttcaaccc cgtccacatc
     2101 atgcgtctcg tcgagatcgt cgtcgggagc gagacgagcg acgagacggt tgagacggcg
     2161 cgtgcggtcg gccagcgaat gaagaaagag ccgatcacgg tgcgcgacgt gccgggtttc
     2221 gcgtcgagcc gtctgggcgt ggcgctcggt ctggaagcga tgcggatgtt ggagcagggg
     2281 gtggcgagcg cgaaagatat agataccgcg atggaactcg gctacaatca ccccatgggg
     2341 ccgctccgcc tgactgatct cgtcggcctc gatgtccgtc tgagcattgc cgaatatctg
     2401 caccgcgcgc tcggttcgga gacttttcgc ccgccggaaa tcctgcggcg catggtcgcc
     2461 gaaggccgtc tcggcaaaaa gagcggcgag ggtttctacg actggaacga ggaagaacag
     2521 gtgagcgggc ggcgttgagc ggttaaaact tatccccgcc gtctctaccg cctgtcttct
     2581 tgctgactgc tgatcgctaa ctgcttcttt tatgaccacg ccgaccaacg aagagagacg
     2641 ttttgcgttg cgcatggcgc tgcgcctgcc catcgtcgtc tccggtcgtg cggaggacgg
     2701 ggcggcgtgg agcgagccga ccgagacgga cgacatctcc accaccggcg cactctttca
     2761 tctcaaccag aaagtgacgc gcggcgaaca actctacctg cgcgcacacc gccccgacgg
     2821 cgcacccatc gaagtcaccg cacgcgtcgt ccgcatcgcc tccgccatct acggcacggc
     2881 gcgcatcggc gtcgccatcc tcgaacccac ggaaaactgg ctccgcctct tcgtctcatg
     2941 ggtcgcagac gatcaagtcg ctcaagacag tgatgggtga caagtgacga gttaaagcag
     3001 aagaattatc tttaacttat cacgcatcac tcatcactgt tttatctcac tcgtcacccg
     3061 tcactcatca ctgcttttaa gagatttacc atgctcaaaa gaatattttt cgccctcgcc
     3121 ctgtttgccg ccctctgctg ctccgcagtc cacgcgcaag actgtgctcc gccgcccctc
     3181 gtcgccaacg cgaaatcggc caacctcttt acgcccgaac aggagatgat cttcggcgag
     3241 ttgacgctgc aaaacatgtc tggcgagatt cgcttcctgc gcgacgaggc gttgcaggct
     3301 tacatcaacg ccatcggcgg caaactggcg aagcacctgc cgcccatcgg cctgcgcttc
     3361 cagtttcacc tcgttgacct gcccgaaacc aacgccttca acattcccgg cggccacgtt
     3421 ttcctctcac gcaaactcgt cgctttcgtc aacaacgaag acgaactggc gggcgtcatc
     3481 gcgcacgaac tcgggcacgc cgtcgtccgc cacggggcga cggacatgtc cgatgcgctc
     3541 cgcaaaattc tcaatatcag cgcactcggc gaccgcaaag acatcaccga aaaatataac
     3601 ctgctgatcg agcgtgcgcg cacgaagact ttttcgcgca aacgcgacca tgaaaacgag
     3661 cagcaactcg aagccgaccg cgtcgggctg tttgcgatgg tggcggcggg ctacgacccg
     3721 gcggccttct ccgccttctt cgaccgcctg accgaaagcg agggcaagac cggcggctgg
     3781 ttttccgatc tcttcggcaa gacgcgcccg gagcacaaac gcctgcgcga gatgatccgc
     3841 gccacagaga aacttgcccc ctcctgccgc gatgggcgca ccgcgaaaac gacgggagat
     3901 ttcctgaagt ggcaggcgga tgtcgtctcg ttccgcgaga cggggcgcaa ggaagagttg
     3961 cccggcctgc tctggaagaa agagcttgcg cccaaactgc gcagcgacgt ttcccacttc
     4021 gcctttagtc aggacggaaa atttctgctc gcgcaggatg acttcgccgt cacggtcatc
     4081 gaccgcgaac cgctccaggt gctctttcaa attcccgtcg aagaggccaa cggagcctcg
     4141 ttcacgcccg acgggcgatt cgtcgtcttc accaccgaaa atctgcgcta cgaaaaatgg
     4201 agcgtcgccg agcgcaagcc cgtggaggtg cgcgagttgg ttgtgaggcg cgattgctgg
     4261 gagcacaaac tttcgcccga cggaaactat ctggcctgcg tagacacttc aaccaccatc
     4321 aatgtgctcg aaaccagaac gggcaagcgc gtctgggaga agaaagagtt ctaccccctg
     4381 acggcgttcg agttcttttt ctggctcgcg tcaagcagag gcgaagccga taacaagacc
     4441 aacttttttc gcatcgagtt ttcgcccgat tcgcgctacg tgctgttcag ccgctccgac
     4501 aagtttcgct tccgcatcag catagacggc atgaccgccg ccgcttcgga gaacaccgcg
     4561 ctcgcgctcg acctgacgac gctccaaccg gcggagatag gcggcgacct gaaaaagatc
     4621 gcgtcgcgcc cttacgcctt tttgaacgcg ggcgaggtgc tcggcatgcc ctcgcggaag
     4681 atggaagagg caggcgtgtt ctccttcccc gctggcaagc gtctgcaaaa gttcaccttc
     4741 gccgcgcagg agatcaagcg cacggccaac ccttcctacg tcatcatcaa gccgctggcg
     4801 aacgcgcgca tgggcgtctt cgatctgaaa aaagggatca tcacctccgg catgaacaag
     4861 gctgacggca cgctctggaa cgacctcatg gccttcgaag cgaccaacgg caaaatcctg
     4921 ctccgcgaag tgaagtacaa cgaggagaag aagagtttcg acggcaaaga gatcggcacc
     4981 gtagagattc ccgtcggctc gctcagcaat ctcaacgccg ccgaagtctc cgataatttc
     5041 aactggctcg tcctgtcctc aaagactcgc ggcgggctgt ggaatctcga aaccggcgag
     5101 cgcaaactct acgtgcgcgg tttcaaaggc ggaattgtcg ccaacgacgg cggcggcgtc
     5161 gcagactttg ccaagctcga cgacacgcaa cacagtctgg tcttgatgaa tgcgctcgac
     5221 cagacggtca cacccataca ggagttgccg gagaaaggtg cgcagcaata cgggcgtttc
     5281 gtgctgctgc gaagcagtct gaaagagaag aaagaggagc cgaaaagcgt ctccgccttc
     5341 ttcggtggag acgagtcgga cggttcggga ctgcgccgcg aggtgcgctt cgagttgaaa
     5401 gatttcattc agaacaaagt gatctggtcg cgcgagtttc cgaaggccgc gcccgaatat
     5461 tctttcgatg aattcagcgg gcgtctgatc ttctactggc ggctcggcag cgaggcgggc
     5521 aaagccaagt tgagagagtc aaccgaactg caagccaaag ccgacgcgct cggcaacaag
     5581 accgacgatt atctggtcga agtcgtggac gctttcgcgc agaagacggt cggcctcctg
     5641 ctgctcgaaa ccggcaacgg ctcattcagt gtcggcgcgg gtctatcgga agccgactgg
     5701 ctggtgctgc acgattcggg aaaccgtgtg ctcgtctatt cgatcaagag cggagatttg
     5761 cgccatcgtt ttttcggcag cgtcgcggcg atcaacccga agaagaatca actcgccgta
     5821 gagaatctgc cgggcgaagt cgcgctctac gacctcgaca gcggcgaacg gcagaccacc
     5881 ttcgtcatcg gcggcagcgc ggctttcgtc aggttcaatc tcaagggcga caggttcttc
     5941 gtcctcagcg acgctcaatc ggcctacgct ttcgacttga acaaactcgc gcccaaaacc
     6001 tcaccggcga aataaacgcg agatttcgag tttcaggttg cagatcgcct gttctttggt
     6061 tttaactgga gaactgcaac ctgaaaccta tttttcggag aagattataa tgtcgcaatc
     6121 ttacgaaact ctgctggtcg agcggcgcgg gcgcgtcgcc atcatcacga tcaaccgacc
     6181 tgagaaacgc aacgctttga acatccagac gcgcgccgag ggcgcagccg cgctcgaaga
     6241 gttgcgcgag gacgagacgg tacgcgtcgt cgtctttacg ggcgcgggcg ataaggcttt
     6301 catcgcgggc gcagacatcg cggagtttgc cggtcgcacc gccaacgtcc agcgcgacgt
     6361 gatgacgggc cgctcgctct tcaccgccat cgacactttc ccgaaacccg tcatcgctat
     6421 gatcaacggc tactgcctcg gcggcggctg cgaactcgct ctggcctgcg acctccgcat
     6481 cgcgagcgag cgcgcctctt tcggccagcc ggagatcaac ctcggcatca tccccggcgg
     6541 cggcggcacg cagcgtctca cgcatctggt cggcgaaggc aaagcgatgg agttgatctt
     6601 gacgggcgag atcatagacg cgcagacggc ctacgcttac ggcctcgtca acatggtcgt
     6661 cccggcggaa gaactcgaag cgaagactct ggagatcgcc aaccgcatcg cggagaagag
     6721 tccgatcgcg ctccgcatgg cgaaggaggc cgtcaagacc gcctcgcgct cgacgctcga
     6781 cgaaggacta cgccgcgaga tcgatctctt cgccctctgc ttctccagcg aagacaaaga
     6841 cgaaggcgtc aaagccttca tggaaaaacg caaacccgag ttcaaaggac gctaactccg
     6901 gccaccgctc aagccgccgg ttcgacgccc acacgaataa cctatcgttt cccggatcaa
     6961 ataagaggcg tcctgctcgc taacaggccg cctcttattt tttcgtctcc acccgccgca
     7021 acgccagctt gacgagtttt cgcacgctaa gctaatctct ccattcgtcg cccacacgat
     7081 aaaaccaacc aaggggccgt agctcagctg ggagagcgca tgactggcag tcatgaggtc
     7141 aggggttcga tccccctcgg ctccaccatt atcatcaaga acttacggca gccggaaacg
     7201 gctgctgttc ttgtttcggg gcatatttgg ggcgcgcttt tctgcccgac aggctggccg
     7261 ctgccacggc gttctgtttg ccccagtcgg tcgcgtgcgt gtaacggcgc gtcgtgttcg
     7321 gattcgagtg tcccatcagc tccgcgatgg tcgattcgct ccacccgtcc tcggccaacc
     7381 tggtgccgaa cgtgtggcgc agatcatgcc agcggaggcc gttaatgccc gccagacgac
     7441 acgcagtgcc aaacgctttt ttaaggacag cgtaaggctt gcccgtctct ggattgatga
     7501 acacgtattc tacactcgaa ggcgcgcgcc tgagcgcgag cagggtgttt cttacctctt
     7561 cattcatcgg caccggataa tctttccccg tcttagaatt cggcacgtag atgacgttac
     7621 gctgaaaatc aaccttctcc caacgaaggt tcaactggtc acctcggcgc atgccggtgc
     7681 cgagggcgac ggtaacgaat ggccgaaggt gcgcccgctc gccggtcaaa gtagccatca
     7741 gtctcagctc ctcttcgtcg agcaggtagc gcgtgcgctt attattctcg gccaacaact
     7801 cgacatcctg acacgggtta gtcgcgacca ttttgtgtct gatcgcgagg gtgaaaatac
     7861 ttgagagcaa ttgaagttcc cggttagtgc tcgagggttt acgagtcgtt cccctgttcg
     7921 tgatagactc gcgccgttcc cgtttgtatt tttctatcag caagggcgaa atctgtgcaa
     7981 aggttttgcc cttgaaccat tcacagagca cgatcactgt gtaacggatg ttggtttccc
     8041 acgaacgcct attctcacgc gcccaaggtt tgtaaacctc ttcgacgaat tgaacgaaat
     8101 cttggccgcc gctcggcctc ccatatcgcc cctcgaagac atctttcttt gcctcgagtt
     8161 ccgcctgctc ggcttcgtac ttggtgcgcg cctcgggaat ggcagcacgg tatcggacgc
     8221 cgcgaatggt gaaggcgtat ctccaccgtg gattcttcgg cgtccctctg ttttcaactg
     8281 gcatggtttt cctttcccgg aattcgcagc cacaccgggc cgcatgctac agcatccaca
     8341 ctctcaaggc actatctgcc ggcgcactcc ttcgtccact cgtcaatctc acgagcctcg
     8401 aagaggagct gccggccagc tttgcgaaaa gggatcttct tcttatgcac gagctgatag
     8461 atggtgctcg gccgacaccg gagcaaggtg gccacttcgg caacggtcag gtactgagac
     8521 aagctgtgcg ggagtggagc atctgccgga agcttcaagt gaggcttcgg agcatccgtt
     8581 cgcgatggac gcccactact ttctttggcg gtcatgattg aaacccttct cgggaggtgg
     8641 tggtcaacag agcggcctcc gggtgtgata gttcgattaa cttcctgaaa ttagctttca
     8701 cctggtggcg cgggcgcgcg ccttatcggc cttcgtcacg tagcgcttgc cggtcttagg
     8761 ccgatagtga gagcgacgcc acgcggcatg gcagcgctta cattctgggc gacggccttc
     8821 acgacaactt tgctccctgt agaaagccca gagcgccttg cgcacgccgc aatgccggca
     8881 ccgcttgaac tgcccgccct ggtgctccct cgagggaaat ctgttgagaa aatcggcgtt
     8941 ttctgccatt tcggcaccgt ctcaaaacaa ctcactacca tcagacccgc ataaacactg
     9001 caccaaatca aatagtcctg ctgatgaaat gaaattaatt tctacagcta gcgatactgt
     9061 gggcgtcgca cgttaccctc agtctttcca ggtctgggaa ggacccgcgc gcctatgagc
     9121 gcgatcacga tttgagaaaa aagctcgaaa actcgtgact cgtgacgagc cacgttaccc
     9181 tgacaggact gacgcctacg ctccaccgct gccctccgtg ctgcgctccc gctctcgtct
     9241 ctcagtcttc cgtgctgcgg caccggcgct ggccaatgcc gcgtccgtgt ctatgagcag
     9301 gtgagagacg atctgctccc agtctgacgg gcgcgtgccg gccatgcacg cgcgccagcc
     9361 gcgcagaagt gaatatgggt ccggagatgg ctgcgcctct cgctcgctct taacttctcg
     9421 ctccagggct tcgaatagct cacctgctat ctgctcgaca tcatcaagat cgtccctggc
     9481 atcgctgatg acgtccttca atacctccag gtaggtgagc gcgacctcgg tcgggatgcg
     9541 accctcgatg ctcacctgct catcgacgag gaagaggcag ccattcagcg aatcgacttg
     9601 cgtctggaac tgctcgcgcg cttcctgtag cctgccgttg cgtgccaggg ttgctcctac
     9661 gtagatgaag cctcctctag tgaaaaactc ctcctgctcg tcattccctt gaatctcgct
     9721 gttctctttg tcgctcatcg atgtctcctc tgatcatgta gcgcaagctg tgcgccccat
     9781 tagcgtccaa ttccgtccaa gttgcttgag cactggaagc ctagccggat ggcccgtcat
     9841 gccccgctgg tgactcagaa tctccacctg tgcggcgaat ctctcgcttc cgcgcgacgg
     9901 ccgcgtaggc acgctcccct tctagctgat gcataaactc ctgatgagtt ttgttgtgct
     9961 tcctcggccc gcacttgatg cagcaggcta ccaggttctc catcacctca aggccgcccc
    10021 gcgacttcgg caccttgagc ctgatttgta actccacccg ctcctcataa gggataggct
    10081 cctcggccca gcgtgcggtc agcgtctctt cgtcgacgaa tccttctagc tccagttccc
    10141 tcaattcctg ataaagctgg taggcggggt ttgaccgcag gtgcgcgccg caataggcgc
    10201 actgctcgcc ctgtgccgcc acgattcgtt cgcgcagcac gtagatctgc gccctgaccg
    10261 ccgcccgctg ggacttcagc tcctcatacc gctgccgctg tttctcgaag tcccgggcaa
    10321 tccgttcctg ctcttcgagt aattcagtgt attcgtcgcg catcttcttc tccgatgaag
    10381 ctgtccgctg ctcaagttcc atccgccccg cgagattagc cggatggacc gtcccgcgcc
    10441 gctgcagcct cggaatctct accccatcga cggctggcat gatcattccg cctgttcact
    10501 acctcgcctc tggccagagc atcggcctcc tcgcgccgca agcccccttc atactgcatg
    10561 atggctgcct gctcctcctc aagcgtcaac tcctcttcca actcttcggg tagctggcca
    10621 gcctgcccgg gcggcccctc agcgccatgc agctcgattt ccacgctccg gacggcaggc
    10681 gtcgtcagcc gctccgcgag ccgttcccac gtggccgcag cctccgggtc cgcctccggc
    10741 tccacgtctg acacagccgg catctcctcg gccagggggg ttaggacaaa atttcctagc
    10801 cccctcttaa cttcatgacc cgcctgcact tgcgcgaggg ttaaattcac cacggactct
    10861 attttcgtct gcacccgcgt ccacccgttc aaatatccct ctggctcctc cccgtcaagc
    10921 agctcctcgc gctggttggc agggagttgc gcggccatct cctccagctt ctcgagattc
    10981 gtcaggatga tcttctggca ggtcttcatc acgtctaccg ccgagcgcgg gcgcttctgc
    11041 ttcctccgct tcatcggggc gtccggaatc atgctgtagt tatcagcggc ggcgatccgg
    11101 atggcctcgc gctgcctcct gcggtccgtc tcgtgccatt gtgaggaaga
//
LOCUS       DASWNS010000420         9782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_456351, whole genome shotgun sequence.
ACCESSION   DASWNS010000420 DASWNS010000000
VERSION     DASWNS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456351"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..582
                     /locus_tag="VGB76_14540"
     CDS             43..582
                     /locus_tag="VGB76_14540"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY0100164.1"
                     /translation="MYPSEEQLRAGALARILERNRLVARVAIARKNGARIVFANGCFD
                     ILHVGHVRYLEAARALGDLLVVGINTDAQVRQLKGAGRPFITERERAEIIASLRSVDL
                     VTIFPEPTVETLLLAIRPDIHAKGTDYTADTVPEREVVRSYGGRVAIVGDPKDHSSSN
                     MIVRLNQPNTAPNEVTSDE"
     gene            779..1861
                     /gene="waaC"
                     /locus_tag="VGB76_14545"
     CDS             779..1861
                     /gene="waaC"
                     /locus_tag="VGB76_14545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEY0100165.1"
                     /translation="MRILIVKLGSIGDIVHTLPSLAAIRHALPRAEIAWAVERRAAEV
                     LRDNPLVDRLIELDTKALRRWRPVSGETLLAPRQQLRRLRASAFDVALDFQGLLKSAA
                     IARLSRASRSYGFARHSLREPASRFLLTETVNVPTNTNVIRKNLALAAGALGISVPQD
                     GDEFEFPLGVFAQHEREAEAAAALVDEKYAILNVGGGWPTKLWSAERYGALADALSAQ
                     HGLRSLVTYGPGERELAESVAGASRSGAAQALSLSLKGFYALARRAKLYVGGDTGPTH
                     LAVAAGAPVVGLFGPTEWWRNGSPRAADICVERTDINCRVACHRRACSVWVCMEIEVA
                     SLLAAASERLARAAVESKRVEALRIA"
     gene            1854..2432
                     /locus_tag="VGB76_14550"
     CDS             1854..2432
                     /locus_tag="VGB76_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100166.1"
                     /translation="MRRGGTWVQRWRVPLGFLVAALFIVLARPRGVTLAIGGAVAVCG
                     LLVRAWASGHIRKNDALAVSGPYAYTRNPLYLGTFILGLGFTIAAAGDWWLLYLVLGG
                     MFAALFLGIYLPVMRVEALTLAQLFGEDYRRYAASVPLLFPRLTPYGGDDGRATRLTF
                     DLSLYLRYREYRAALGLLVGWSVLALKAFFVK"
     gene            2441..3376
                     /locus_tag="VGB76_14555"
     CDS             2441..3376
                     /locus_tag="VGB76_14555"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY0100167.1"
                     /translation="MLRQRLRMLFVLLTLTASCAAVVLAGTHGATTETKDAESAAKPR
                     PLPFAPDEQLTYEGEFSKLLLRGINIAEFKFTVERAQPAAVTVTTSENAVSRSANAVP
                     APANLVFKADAVAKGWFRKLFGIDFHYNSESVVEPTTLRILNNTTRDEQGKRLRTSEA
                     VFDRTHNLLTWTQRNPHDPQSAPRVVSVPLQNASHDLISAIYFLRTQPLEPGRTFELD
                     ISDAGAVYRIPVKVGARKRMKSVVGRVQTIRVDIEIFGAQRLIDRKGEMTLWITDDAR
                     RLPVRARIDTDIGTLDITLKKVSGVNNDAAAAGRR"
     gene            3418..3939
                     /locus_tag="VGB76_14560"
     CDS             3418..3939
                     /locus_tag="VGB76_14560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0100168.1"
                     /translation="MSYIVADMEAGDWEQVRSIYLEGIATGVATFETSAPTWEKWDAS
                     HLRKMRLVARAGDRGDEGDGGEILGWAALSPVSDRCVYGGVAEVSVYVGERGRGRGIG
                     RALLQSLVAASERNGIWTLQAGVFPENAASIKLHLSCGFREVGRRERIGKLNGVWRDT
                     LLLERRSQSVGAS"
     gene            4039..4425
                     /locus_tag="VGB76_14565"
     CDS             4039..4425
                     /locus_tag="VGB76_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100169.1"
                     /translation="MTIRFSRRLAILFGILAPLGETVRRWSTWQENPPNLFDDYIMGA
                     FLLYGAWRAGRDLRSGQRFLAAAWAFTCGLGYYSLFGQLKSWSLHEIDPAPIPSGWVL
                     AIKGVGVALSVLALALSLRRTPESQS"
     gene            4562..5218
                     /locus_tag="VGB76_14570"
     CDS             4562..5218
                     /locus_tag="VGB76_14570"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HEY0100170.1"
                     /translation="MTLQPDGTLKATPPTDVQTASSTNRHPSASEESLPLVLKRDSIQ
                     LLLQSPNKLFLYWSFANDPRVALRSAFGELAAHYRLAVRLVKVESGEEFLLDAPGERM
                     QWFEVYPRHVYRADVGFHAAGRPFVRLLSSGEVRTPPDSASHLSAQEPEFQVKPDEFA
                     RLLDGAGYGSYARGLTNEPDANAGGRSGLAAIIKDASEDASAGQHAAREDAADEYPHR
                     "
     gene            5230..6834
                     /locus_tag="VGB76_14575"
     CDS             5230..6834
                     /locus_tag="VGB76_14575"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HEY0100171.1"
                     /translation="MPHGFFSLILHAHLPFVRHPEHAEFLEEDWLYEAITEVYLPLLD
                     ALNALHETHAAPRLALNLSPTLCEMLADPLLQTRYTRHLENLSALAEKEVARTRLDAR
                     QLLPVARMYRRQLRRAAELWHEVYRRDILSGFRALQAAGVCEIMTCAATHGFLPLIST
                     TEARRAQIEIAVANYQKHFGRRPRGIWLPECAYAEDIETLLADAGLEYFIADTHAILY
                     GEPRPRQGVYAPVKLTNGVAAFARDLATSQQVWSSIIGYPGDANYREFYRDIGWDAPL
                     EYLRPHLHANGERRHLGLKYHRVTGREVPLSDKQPYRPHRASRRAALHAAHFVDERIK
                     QCASLRHALAGGYLPLITSPYDAELFGHWWFEGVQFLGHVFRELARRRDEITTLSPAD
                     YLDTHTRLQTQRLSPSSWGAEGYYKVWLNDTNAWLYPQQHAAEARMTALADQHPAPPG
                     AMLTRALNQAARELLLAEASDWAFQISQATTAEYAARRFRSHIARFNTLASDIERGEI
                     DAARLAEIEGRDNLFAELDYRAYRSR"
     gene            6831..7709
                     /locus_tag="VGB76_14580"
     CDS             6831..7709
                     /locus_tag="VGB76_14580"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY0100172.1"
                     /translation="MRLHTPETTDGEAATPSELPDENFPLVIGHRGASKVAPENTLAA
                     FERALADGADGIEFDVRLARDGVPVVIHDATLKRTAALHTDAPIASLSSTGLSAIDVG
                     SWFNQRFPARAQNAYRRETIPTLARVFESIAPRCRVLYVELKFAAADMRNSAGGVTNF
                     VEKVVAEVRAHSLERRVVIESFALPIVAEVKRIAPDLRTAAAFERRLGRPLVAPRTLL
                     RRARACRADELALGRSLVSRRMIEAARASGLPSVVWTVDHPSWAGRASALGLRAIITN
                     DPARMRAALGDFRGRA"
     gene            7737..8600
                     /locus_tag="VGB76_14585"
     CDS             7737..8600
                     /locus_tag="VGB76_14585"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0100173.1"
                     /translation="MTISASSTDKIFVHEHYAIHYRRAASRAQWAGGEAHPAYAALFV
                     LSGAMRWQAGDGRSGELRESGLLVAAPGESVGARGSRVETLALSVAPSFVLDCAVRAR
                     LTRDDARITFPAQAIEHDPRLGRLARDMADELRAAETGHELIVAALIEQILIQLLRRY
                     ASIRRTDELELSRAGLVDRRIRRAVELMHANLAEELPLEDLAAAAYLSPFHFARLFKK
                     LTGLPPHAYLAALRLERARMLLATTDLTITEVAARVGYANSSHFGKAFRQFNGLTPRA
                     FRSALTTESRG"
     gene            8760..>9782
                     /locus_tag="VGB76_14590"
     CDS             8760..>9782
                     /locus_tag="VGB76_14590"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEY0100174.1"
                     /translation="MAVEKGTEIAPAKGKLGVLLVGLGAVSTTFIAGVEAIKAGLAEP
                     IGSLTQMGTIRLGKRTENRSPAIKDFVPLAGLNDLVFGAWDIFPDSAYDAAMHAGVLE
                     KELLVKLKTPLQKIKPMKAVFDQNYVKRLNGENVKTGENKLELAEQLIAEIEEFKRKN
                     KCERLVMIWAASTEIFLKQHAVYKTLKSFEQAMRDNHKAIAPSMIYAYAALKLGIPFA
                     NGAPNLTVDIPAMMELADQNNVPLCGKDFKTGQTLMKTILAPGLKSRMLGLHGWYSTN
                     ILGNRDGEVLDDPESFKTKEESKLSVLEYILQPDVYPELYKDFSHVVRINDYPPRGDT
                     KEGWDNI"
BASE COUNT         1791 a         3246 c         2947 g         1798 t
ORIGIN      
        1 tttaagttgg gtttaagcgg catttggatt ttggattttc ttatgtaccc gtctgaagaa
       61 caactgagag cgggagcgtt ggcgcgtatc ctggagcgaa accggctggt ggcgcgcgtg
      121 gcgattgcgc gaaagaacgg tgcgcgcatc gtgtttgcca acggctgctt cgacattctg
      181 cacgtcggcc atgtgcgcta tctggaagcg gcgcgcgcgc tgggcgatct gctcgtcgta
      241 gggatcaaca ctgacgcgca ggtgcgccag ttgaaagggg ctggacgccc gtttatcacc
      301 gagcgcgagc gggcggagat catcgcctcg ctgcgttccg tcgatctcgt cacgatcttt
      361 cccgaaccga cggttgagac gctgctgctc gccattcgcc cggacatcca cgccaagggg
      421 acggactaca cggcagacac cgtgcccgaa cgcgaagtgg tgcgctccta cggcggtcgc
      481 gtcgccatcg tcggcgaccc caaagaccac tcctcctcca acatgatcgt ccggctcaac
      541 cagccgaata cagcgccgaa cgaagtgacg agtgacgagt gaccgctgac aagcaggaaa
      601 aagccgtcag acgtgtattg agagaagcgg tttccttccg tagcagtgag tgctcctgct
      661 aattgccctc cgcgttgttg acggttgacg ccgcttgcgt tcgcggcttt tccggtttcc
      721 gattcacccg ccgctgtttt atcttgtcac ctgtcactcg tcgcacgcca ctgtttttat
      781 gcggattttg atcgtcaaat tgggaagcat cggcgacatc gtgcacactc tgccgtcgct
      841 ggctgcgatc cggcatgcgc tgccgcgtgc ggaaatcgcg tgggcggtgg aacgtcgtgc
      901 ggcggaagtt ttaagggaca accctctcgt ggatcgtctc atcgaactcg atacgaaggc
      961 tttgaggcgt tggaggccgg tgtctgggga gacgctgttg gcaccgcgcc agcagttgcg
     1021 tcggctccgc gcctcggcct tcgatgtcgc gctcgacttt caaggtctac tcaaatccgc
     1081 cgccatcgca cgcctctcgc gcgcttcgcg ctcctacggc ttcgcgcgcc attctctgcg
     1141 cgaaccggcc agccgctttt tgttgaccga gacggttaac gttcccacga acacgaacgt
     1201 catacgaaag aatctcgcgc tcgcggcggg cgcgctcggc atcagcgtgc cgcaggacgg
     1261 cgacgaattc gagtttccgc tcggcgtgtt cgcgcagcat gagcgcgagg ctgaagcggc
     1321 ggcggcgtta gttgatgaga agtacgctat tttgaatgtg ggcggcggct ggccgacgaa
     1381 gttgtggagc gcggagcgat acggcgcgct ggccgatgcg ctgtcggcgc aacacggcct
     1441 gcgctcgctc gtgacttatg gacccggcga gcgcgagttg gcggagagcg tcgcgggtgc
     1501 gagccgttcg ggagcggcgc aggctttgag tctctctctg aaaggttttt acgcgctggc
     1561 gcggcgcgct aagttgtacg tgggcggcga tacggggccg acgcatctgg ccgtggcggc
     1621 gggtgcgccg gtcgtcggcc tgttcggccc gacggagtgg tggcgtaacg gcagcccgcg
     1681 cgcagcggac atttgcgtcg aacgcacgga catcaattgt cgcgtcgcct gtcatcgtcg
     1741 cgcctgctcg gtgtgggtct gcatggagat cgaggtggcg agcttgctgg cggcggcgag
     1801 cgaacggctg gcgcgtgcgg cagtggagtc gaagcgcgtg gaggctttga ggattgcgta
     1861 gaggcgggac atgggtgcag cgttggcgcg tgccgctcgg ctttctggtg gccgcgctct
     1921 tcatcgtgct cgcacgaccg cgcggcgtga cgctcgccat cgggggggcg gttgccgtct
     1981 gcggcctgct cgtgcgcgcg tgggcttccg ggcacatacg caaaaacgac gcgctcgccg
     2041 tctccggtcc ctacgcctac acgcgcaacc cgctctatct cggcacgttc atcttaggct
     2101 tgggcttcac catcgcggcg gcaggcgatt ggtggttgct ctaccttgtg ctcgggggga
     2161 tgttcgcggc cttgtttttg ggcatctatt tgcctgtgat gcgcgtcgaa gcgttgaccc
     2221 tcgcacagct tttcggcgag gactacaggc gttacgcggc gtcggtgccg ctgctcttcc
     2281 cgcgtctcac gccttacggc ggcgacgacg ggcgcgcgac gagattgacg tttgatctga
     2341 gcctgtacct gcgctaccgc gagtatcgcg ccgcgctcgg attgctggtt ggttggagcg
     2401 tcctcgcgct caaggctttc ttcgtgaagt gagaatcgag atgctgcgtc aaaggctcag
     2461 aatgttattt gtcctgctca cgctcacggc ctcgtgcgcc gccgtcgtct tggccggtac
     2521 acacggcgcg acgacggaga cgaaggatgc agagagcgcg gccaagccga ggccgctccc
     2581 tttcgcccct gacgaacaac tcacttacga aggcgagttc tccaagttgc tgctgcgcgg
     2641 catcaacatc gccgaattca agtttacggt cgaacgcgcg cagcctgcgg cggtaacggt
     2701 cacgacgagc gagaacgccg taagccggag cgcgaacgct gttccggctc cggccaatct
     2761 cgtctttaaa gccgacgcgg tggcgaaggg ctggtttcgc aaactcttcg gcattgactt
     2821 tcactacaac tcggaatccg tcgtcgagcc gacgaccctg cgcatcctga acaacacgac
     2881 gcgcgacgaa cagggcaaac gcctgcgcac gagcgaagcc gtctttgatc gcacgcataa
     2941 tttgttgacg tggacgcaac gcaacccgca cgacccgcag agcgcgccgc gcgtcgtcag
     3001 cgtgccgctc cagaacgcct cgcacgatct catctccgcc atctattttc tgcgcacgca
     3061 gccgctcgaa cccggacgca ccttcgaact cgacatcagc gacgcgggcg cggtctatcg
     3121 catcccggtc aaggtcgggg cgcgcaagcg catgaagtcc gtcgtcgggc gcgtgcagac
     3181 aattcgcgtt gacatcgaaa tcttcggcgc gcaacggctc attgaccgca aaggcgaaat
     3241 gacgctctgg atcacggacg acgcgcgccg cctccccgtt cgcgcacgca tcgacacgga
     3301 catcggcacg ctcgacatca ccctcaaaaa agtctcaggc gttaacaacg acgccgccgc
     3361 cgccggtcgc cgctaattcg tgtttgagca accggctcag gcgcgcggtt ccaaaatatg
     3421 agttacatcg tggccgacat ggaggccggg gactgggagc aggtgcgctc gatctatctt
     3481 gagggcatcg ccacgggcgt cgcgactttt gagacgagcg cgccgacgtg ggagaagtgg
     3541 gacgcgtcgc acctgcggaa gatgcgactc gtggcgcgcg ccggtgaccg gggcgacgag
     3601 ggcgacgggg gcgagatttt aggttgggcg gctctgagtc cggtgtccga cagatgcgtc
     3661 tatggcgggg tggcggaggt gagcgtgtac gtcggagagc gcgggcgtgg acgcggcatc
     3721 gggcgcgcgc tgctccaatc gctcgtcgca gcatcggagc gaaacggaat ctggacgctg
     3781 caagcgggcg tgtttccgga aaatgccgcg agcatcaagt tgcatctgag ttgcggcttt
     3841 cgagaagtcg gcaggcgcga gcgcatcggg aagttgaacg gagtctggcg cgatactttg
     3901 ctgttggagc gtcgcagcca aagcgtcggg gcgtcttgag agcctgtttc aaattaagag
     3961 cttgtttcaa aactgcgttt tccgcctctc cgcctttcca tttttaccgc gcgcttcggg
     4021 tttgcgttcg aggagattat gacgatccgc ttcagccgca gactcgccat cctgttcggc
     4081 atactcgcgc cgctcggcga gacggttcgc cgttggagca cctggcagga gaacccgccg
     4141 aacctgtttg acgattacat catgggcgcg tttttgctct acggcgcatg gcgtgcgggg
     4201 cgcgacctgc gcagcggtca acgctttctc gcggcggcct gggcttttac gtgcggcctc
     4261 ggctattaca gtctcttcgg gcaactgaaa agctggagtt tgcatgagat tgaccccgcg
     4321 ccgattccct ccggctgggt gctggcgatc aagggcgtgg gcgtcgcgct ctcggtgctg
     4381 gcgctggcgt tgagtctcag gcgaacgcct gagagccagt cctgacgaag ccgtgcgctc
     4441 cgcgatcctc tccgcacgct tttgttgaca ccacgcgggc aatcacttac aacttaactc
     4501 gctccgccta tcacacccgg aagagagcag gcggcggcac gcccgcagca aacgagacga
     4561 catgacgtta cagcctgacg gcacgctcaa agcgactccc ccgaccgacg ttcaaaccgc
     4621 gtcttcaact aatcgacacc catccgccag cgaagagtct ctgccgctcg tgctcaaacg
     4681 cgactcgatc caacttcttc tgcaatcgcc caacaagctc ttcctctact ggagtttcgc
     4741 caacgatccg cgcgtcgctc tgcgttcggc cttcggcgag ttggccgcgc attaccggct
     4801 ggcggtgcgt ctggtcaagg ttgagagcgg cgaggagttt ttgctggatg cgccgggcga
     4861 gcgtatgcaa tggttcgaag tctacccgcg ccacgtctat cgcgccgatg tcggcttcca
     4921 cgcggcggga cgccccttcg tccgcctgct ctcttccggc gaggtacgca cgccgcccga
     4981 tagcgcgtcg cacctgtcgg cgcaagaacc ggagtttcag gttaagccgg atgagttcgc
     5041 acgccttctg gacggcgcgg gttacggaag ctacgcgcgc gggctgacga atgagccgga
     5101 cgctaacgcg ggcgggcgca gcggccttgc agctatcatc aaagacgcat ccgaggacgc
     5161 gagcgcaggg cagcacgccg cacgcgaaga cgccgccgac gaatacccgc accgctgatc
     5221 tcttaagacg tgccgcacgg atttttcagt ctcatccttc acgcgcacct gcccttcgtg
     5281 cgccatcccg aacacgcaga gtttttagaa gaagactggc tctacgaagc catcaccgaa
     5341 gtctatctcc cgctgctcga cgcgctcaac gcgctgcacg agacgcacgc cgccccgcgt
     5401 ctcgccctga acctctcgcc cacgctctgc gagatgctcg ccgacccgct cctgcaaacg
     5461 cgctacacgc gccatctcga aaacctctcc gcactcgccg aaaaagaagt cgcccgcacg
     5521 cgcctcgacg cacgacaact gctccccgtc gcgcgcatgt atcgcaggca actgcgccgc
     5581 gccgccgaac tctggcacga agtttaccgg cgcgacatcc tcagcggttt tcgcgcgctg
     5641 caagcggcgg gcgtctgcga gatcatgacc tgcgccgcga cgcacggttt cctcccgctc
     5701 atctcgacga ccgaagcgcg ccgcgcgcaa atcgagatcg ccgtcgccaa ctatcagaag
     5761 catttcggtc gtcgcccgcg cggcatctgg ctgcccgagt gcgcctacgc cgaagacatc
     5821 gaaacgctcc tcgccgacgc cgggcttgaa tacttcatcg ccgacacgca cgccatcctc
     5881 tacggcgagc cgcgcccgcg tcaaggcgtc tacgcgcccg tcaaattgac gaacggcgtc
     5941 gccgcttttg cgcgcgatct ggcgacgagc cagcaggtct ggagttcgat catcggctat
     6001 ccgggcgacg ccaactaccg cgagttctac agggacatcg gctgggatgc accgctcgaa
     6061 tatctccgcc cgcatctcca cgcgaacggc gaacgccgcc acctcggcct caaatatcat
     6121 cgcgtcacag gccgcgaagt gccgctctcc gacaaacaac cttaccgccc gcaccgcgcc
     6181 agccgccgcg ccgccctcca cgccgcgcat ttcgttgacg aacgcatcaa gcaatgcgcc
     6241 agcctgcgcc atgcgctcgc cggaggctac ctgcccctca tcacctcccc ctacgacgcc
     6301 gaactcttcg gccactggtg gtttgagggc gtacagtttc tcggacacgt cttccgcgaa
     6361 ctcgcgcggc gtcgagacga gatcacaacc ctctcgcccg ccgactatct agacacgcac
     6421 acgcgcctcc agacgcaacg gctctcccct tcctcttggg gcgcggaagg ctattacaaa
     6481 gtctggttga acgacacgaa cgcctggctc tacccgcaac agcacgccgc cgaagcgcgc
     6541 atgaccgcgc tcgccgacca acatcctgcg ccgcccggtg cgatgctcac ccgcgcgctc
     6601 aatcaagccg cacgcgaact gctcctcgcc gaagcatccg attgggcgtt tcaaatctct
     6661 caagccacca ccgccgaata cgccgcgcgt cgcttccgct cgcacatcgc acgcttcaac
     6721 acgctggcct ccgacatcga acgcggcgag atcgacgccg cgcgtctcgc cgagatcgaa
     6781 ggccgcgaca acctcttcgc cgaactcgac taccgcgcct atcgttcaag atgagactcc
     6841 acacgccaga gacgacggac ggcgaagcgg cgacgccttc agaattgccg gacgaaaact
     6901 tcccgctcgt catcggccat cgcggcgcat cgaaggtcgc ccccgaaaac actctcgccg
     6961 ccttcgagcg cgccctcgca gacggcgcgg acggcatcga gttcgacgtg cgactcgcac
     7021 gcgacggcgt acccgtcgtc atccacgatg cgacgctcaa acgcaccgcc gccctccaca
     7081 cggacgctcc aatagcatcc ctctcatcca ccgggttgtc cgccatagat gtcggctcct
     7141 ggttcaacca gcgattcccc gcccgcgcgc agaacgccta ccggcgcgaa actatcccca
     7201 cgctcgcccg cgtcttcgag tcaatcgccc cgcgctgccg tgtgctctac gtcgaactaa
     7261 aattcgcagc ggcggatatg agaaactccg ctggcggcgt cacaaacttc gtggagaaag
     7321 tcgtggcgga agttcgcgcg cactcgctgg agcggcgtgt cgtgatcgaa agtttcgcgc
     7381 tccccatcgt cgcagaggtg aagcgcatcg cgcccgactt gcgcacggca gccgcctttg
     7441 aacgacggct cgggcggcca ctcgtcgctc cgcgcacgct gctccgccgg gcacgcgcct
     7501 gccgtgcgga tgaactcgcg ctcgggcgtt cgctcgtctc gcgtcgcatg atcgaggcgg
     7561 cgcgagcctc cgggttgcca agcgtcgtct ggacggttga tcatccgtca tgggcgggac
     7621 gcgcgagcgc gctcggcctt cgcgccatca tcaccaacga tccggcgcgc atgcgtgccg
     7681 ccctcggaga ctttcgtggc cgagcatagt tttgaaataa gctcccggtt tccggcatga
     7741 caatttccgc ctcttcaaca gataagattt tcgtccacga gcactacgcg attcactacc
     7801 ggcgcgcggc gtcgcgtgcg cagtgggcgg gcggcgaggc gcatccggct tacgccgcgc
     7861 tgttcgtgct gtccggcgcg atgcgctggc aggcaggcga cgggcgcagc ggcgaactgc
     7921 gcgagtcggg gctgcttgtc gccgcgccgg gcgaaagcgt cggtgcgcgc ggaagccgtg
     7981 ttgagacgct cgcgctctcg gtcgcgccct ctttcgtgct cgactgcgcc gtccgcgcac
     8041 gtctcacgcg cgacgatgcg cgcatcactt ttcccgcaca ggccatcgag cacgacccgc
     8101 gcctcggacg cctcgcgcgc gacatggccg acgaactgcg cgccgccgaa accggacacg
     8161 aactcatcgt cgccgccctc atcgaacaaa ttctgattca actgctccgc cgctatgcca
     8221 gcattcggcg cacggacgaa ctcgaactct cccgcgccgg attggtagat cgacgcatcc
     8281 gccgcgccgt cgagttgatg cacgccaatc tcgccgagga actgccgctc gaagacctcg
     8341 ccgccgccgc ctatctctcg ccctttcatt tcgcgcgcct cttcaaaaaa ctgaccggcc
     8401 tcccgcccca tgcctacctc gcagccctcc gcctcgaacg cgcacgcatg ctcctcgcca
     8461 ccaccgacct gaccatcacc gaagtcgccg cgcgcgtcgg ctatgccaac tcaagccact
     8521 tcggcaaagc ctttcgccag ttcaacggcc tcaccccgcg cgcctttcgc tccgcactga
     8581 ctacagagtc cagaggttag aggttagtga aaatcagttt gcggctactg actcaccgct
     8641 gtcccctgtt gttaagcaat tctgcgacga caatgcgcaa gatttgacga gacgcgggcg
     8701 ggcgcgggcg cgtatcctcg atgcgtaata attttccagt tggaggatgc cgaacaaata
     8761 tggcagtcga aaaagggaca gagatcgctc cggcgaaagg caagctcggc gtgttgcttg
     8821 tgggcttagg cgcggtaagc acaacattca tcgcgggtgt cgaagcgatc aaggcggggc
     8881 tggccgagcc gattggcagc ctgacgcaga tgggcacgat tcgtctcggc aagcgcacag
     8941 agaatcgctc gcccgcgatc aaggatttcg tgccgctcgc aggcttgaac gatctcgtct
     9001 tcggcgcatg ggatatcttc cccgattccg cctacgacgc agcgatgcac gcgggcgtgc
     9061 tcgaaaaaga actgctcgtc aaactcaaga cgcctttgca aaagatcaag ccgatgaagg
     9121 ccgtctttga tcaaaactac gtgaaacgtc tgaacggcga gaacgtcaag acgggcgaga
     9181 acaaactcga actggccgaa cagttgatcg cggagatcga agagttcaaa cgcaagaaca
     9241 agtgcgagcg gctcgtcatg atctgggcgg cctcgaccga aatcttcctc aagcagcacg
     9301 ccgtctacaa gactctcaag tccttcgagc aggcgatgcg cgacaatcac aaggccatcg
     9361 cgccttcgat gatctacgcc tacgccgcgc tcaaactcgg catccccttc gccaacggcg
     9421 cgccgaatct cacggtggac atccccgcca tgatggaact ggcagaccag aacaacgtgc
     9481 ccctctgcgg caaggatttc aagaccggcc agacgctgat gaagaccatc cttgcgcccg
     9541 gcctgaaatc gcgcatgctc ggcctgcacg gctggtactc gaccaacatc ctcggcaacc
     9601 gcgacggcga agtcctagac gatcccgaat ccttcaagac gaaagaggaa tcgaaactct
     9661 ccgtcctcga atacatcctg caaccggacg tctaccccga actctacaaa gacttctcgc
     9721 acgtcgtgcg catcaacgac tatccgccgc gcggcgacac taaagagggt tgggacaaca
     9781 ta
//
LOCUS       DASWNS010000421        10372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_198988, whole genome shotgun sequence.
ACCESSION   DASWNS010000421 DASWNS010000000
VERSION     DASWNS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198988"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /gene="mutS"
                     /locus_tag="VGB76_14595"
     CDS             <1..1545
                     /gene="mutS"
                     /locus_tag="VGB76_14595"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY0100175.1"
                     /translation="TPQEMLALGQSLETLPRVRAVLQQDQKRFAALLAALPACAAAAR
                     VIREAIADELPKRGEGRGIIRAGYSDELDKLRELLRNGKTYLAEMEQRERARTGIKSL
                     RVGYNKVFGHYIEVTRPNLHLVPESYTRKQTLAQAERFVTLELKEHESLVVNAQERIG
                     ELETNIFRQICAEIGRSRDEILAAASVVAHLDVVASFAEVAAEHKYVRPVVSETPVLR
                     LTAGRHPVIERMFAEGEFVANDLKLGGENAPEIALITGPNMSGKSTYLRQAALIVLMA
                     QAGSFVPATYATIGLCDRIFTRIGLYDRLGSGESTFMTEMVETAQILHHASERSLILL
                     DELGRGTSTYDGLAVARAVLEYIHNHPQLRAKTLFATHYHELTALASVLPRLENLHVE
                     IAEEGSELVFLYKISPGSAEKSYGVYAAKLAGLPRPVVRRAEELLREYESQGGERHFP
                     GESAAKQTAQLPARERESKIIQALMSLDPDALAPVEALMKLFELRRMAEECVDETGKV
                     RAIRSA"
     gene            complement(1643..2551)
                     /locus_tag="VGB76_14600"
     CDS             complement(1643..2551)
                     /locus_tag="VGB76_14600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0100176.1"
                     /translation="MSVPTARETHARVRARESALKNSTARLSILAAAFLILLKTGTGW
                     LTGSIAVWASLLDSTMDILASTINYFAVRAASRPPDEDHAYGHGKAESLAGLFQAAVI
                     TLSGLFLIFEAIRRVIHPQPTEQESIGVATMLIAIVVSLALVARLRRAAELTDSPALS
                     ADAVHFASDVWTNGGVLAGLVLIYFTGWNIVDPLISIAISIYILASALSVGRESVDAL
                     MDRRLPPEVDKKVAEIVGRFRGEGVLGFHDLRTRRSGSQKFIDLHLEVERDKTLEEAH
                     AASVIVLRTIEAEIPRAKVQIHTDPA"
     gene            complement(2624..3586)
                     /locus_tag="VGB76_14605"
     CDS             complement(2624..3586)
                     /locus_tag="VGB76_14605"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0100177.1"
                     /translation="MNATTVTTTAPVQSKSFKPHGALIAVQLIFGTWPIVGKLVLRSL
                     SSTGLVTFRIVGATLAFLVIRRATNHVSIKGRADFARLLLYSLLGVVLNQFLYVKGLE
                     LSTVINSALIGSTIPIFTLLVSVFLGYESGSWRVAVGLFLAACGVVYLINPAEASFSR
                     ETNIGNFILVLNSMAYGAYIAISQDMIKRYGALTVITWIFIFGSFITLPIGGYHLSKF
                     SLSSVPVLAWCAMLYIILVPTVAAYYLNAWALGRVAPSIVAIYIYLQPLIAFAVAPLI
                     LGEQLNSRTWLAAALIFAGLATVTLRSRSRVLEEVSERPEALGH"
     gene            complement(3639..5111)
                     /locus_tag="VGB76_14610"
     CDS             complement(3639..5111)
                     /locus_tag="VGB76_14610"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEY0100178.1"
                     /translation="MATQVVMPKLSPTMEEGQLARWLKNEGDKVSVGEPIAEIDTDKA
                     TMEAQALTTGVLRKILVEAGATVPLGQVIAIIGEADEDISALVSQAESGEAAAANKKS
                     IEESVEAGQDGAQRELAGVVVGGARTQATIAGEPIKGEAGGAPSTGNAPEPPPAQTST
                     PEAASPGGDNASGNGQRAGGRIVVSPIAARMAAEANINLKTLTGSGPSGRIIKRDIEA
                     AINAGQQQATADNAQGRTQAEPTELRAAEARAPQQPAQLPGVEGASPYRDEPVSTMRS
                     TIARRLASSIGPVPHFFLTTEIEMDRTAELRASLNALDPQNKVSINDIIIKVAAVALM
                     QHPQVNASFQDKTIRYYEHADIGVAVAIDEGLITPIVRGAERKSIGQISREVRELAER
                     ARTRKLQPEEYMGATFSVSNLGMFGIEEFTAIINPPEAAILAVGAMSQKPVVRDGEMA
                     VRQMMRVTMSCDHRIVDGATGAKFLQTFKKILENPLMLML"
     gene            complement(5199..6182)
                     /locus_tag="VGB76_14615"
     CDS             complement(5199..6182)
                     /locus_tag="VGB76_14615"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEY0100179.1"
                     /translation="MAVITIREALNQALSEEMHRDENVFLLGEEVAAYQGAYKVSKGL
                     LEEFGEKRVIDSPITELGFAGLGVGAAMVGLRPVIEFMTFNFSILATDQIINSAAKML
                     YMSGGQFKIPIVFRGPNGSAYQVSSQHSQALESWYAHFPGLKVVMPSTAADAKGLLKS
                     AIRDDDPVIFMEQERMYGLKGEVPDDPDFTIPLGVADIKREGKDATIVARSLTVPLAL
                     KAAEELEKDGVSVEVVDPRTIRPLDIETIINSVKKTNRVVIVEESHPFCGVGAEISAQ
                     IMERAFDYLDAPVKRLSSADAPMPYAKNLEQLALPDVARIVAAVREVAYID"
     gene            complement(6280..7425)
                     /gene="pdhA"
                     /locus_tag="VGB76_14620"
     CDS             complement(6280..7425)
                     /gene="pdhA"
                     /locus_tag="VGB76_14620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY0100180.1"
                     /translation="MNELKVTPLKPRDQSTGQAAVAGIADLDSTDTINPAELREEHEQ
                     RQQHQHQQIEEIDRETLRNMLYQMVLIRRFEEKAAEAYTLGKIGGFCHLYIGQEAVAV
                     GALSAIRPDDYVMTSYRDHGHALARGTSADEVMAELYGKAGGCSAGKGGSMHLFDSDA
                     YFLGGHGIVGGQIPLATGVAFAAKYKGTGQVSLVFFGEAAVNQGALHESFNMAQLWKL
                     PCIYFCENNQYGMGTSLARAMSLQDVSQKAHAYGMASEFVDGMDVLAVREATERAVER
                     ARKESLPTLIEVRTYRFMGHSMSDPGNYRTRAEIEKHQERDPVKLFSDSLRHKGVIDD
                     AALKELEDRVRAEVEHSVKFAEESPLPAPAELFTHIYANPIKVTSEG"
     gene            complement(7667..10174)
                     /locus_tag="VGB76_14625"
     CDS             complement(7667..10174)
                     /locus_tag="VGB76_14625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0100181.1"
                     /translation="MPNANWKSLALLCATAALAAALLVFSLDGLLSLTTNGYLWCGAL
                     LALTFAASLLNVPLVNADGKSHGSKSVADALIFLAAMMFGTAPAAILAGADGFMSSRK
                     TGDRAGLVFRTAAAVISTCIAVMVYNLLTRLTIGGATSAEAHNAPLERLIFPLFVLAI
                     VQFGLNTLLSAFYVAYEAGTKKLEFSRESLVWTSMTQLAGAAAAALFYSSFIGSGMSF
                     LFVGVLIVSLVYLLFRFDEKRVSETRRGEEAKLRHIQEIADLHMNTIESLAIAIDAKD
                     QTTHGHVRRTQIYAMELGRILGVGEQELHALRAGALLHDVGKLAVPEYILNKPGKLTA
                     AEFDKMKIHTVVGGDIIKRVNFPYPVEDIVRYHHEKWDGSGYPKGLRAEQIPLVARII
                     SVVDFYDATRCDRPYRVGMKREDSLALLRRMASTSFDPRVVDMFVSNIDYFDNLIAEH
                     DIQEQVASEDDAKLNGAPDAGLASDILGSASDGDSGFRSIAEAQREVFALHEIAQTIG
                     SSLNLQDTVTLVSSKLRSIVPFDTCIIYLVDDRSGKAVAAHASGEHAEAFARRRITIG
                     EGISGWVIANARSMCNTSPELDLVGLPEEVSSNIRGVLVSPLIREDGAFGAITVYSKT
                     RATYTTEHVRLLESVSQHASIALNNALTFEKTKESALTDTLTDLPNARSFHLALEQRI
                     AECQRLNREPLSVLSMDLDNFKQINDTHGHAIGDRLLASVAAIVKKQFRQMDILARYA
                     GDEFVAIMPMASGDVAEMVADRIRAAVESHEFTIRTGRTTRIGISIGIACFPTDGETS
                     ESLLSQAGRNMQQDKHGRKLNPPSDPQNMVLSLDSFR"
BASE COUNT         2115 a         2998 c         3202 g         2057 t
ORIGIN      
        1 acgccgcagg agatgctcgc gctcggtcag agtcttgaga cgctcccgcg cgtgcgcgcc
       61 gtcttgcagc aggatcagaa gcggttcgcc gcgttgctcg ccgcgctccc ggcgtgcgcg
      121 gcggcggcgc gtgtcattcg cgaagccatc gcggacgagt tgccgaagcg cggcgaaggt
      181 agagggataa ttcgcgcggg ttattccgac gaactcgata aactgcgcga gttgttgcgc
      241 aacggcaaga cttatctggc cgagatggaa cagcgtgaac gcgcacgcac ggggatcaag
      301 agtctccgcg tcggttacaa caaggtcttc ggtcactaca tcgaagtcac gcgcccgaat
      361 cttcatctcg tccccgaaag ctacacgcgc aagcagacgc tcgcgcaggc cgaacgcttc
      421 gtcacgctcg aactcaaaga gcacgaatcg ctcgtcgtca atgcacagga gcgcattggc
      481 gaacttgaga cgaacatctt ccggcagatc tgcgcggaga tcgggcgcag ccgcgatgag
      541 attctcgccg ccgcttccgt cgtcgcacat ctcgacgtgg tcgcttcgtt tgccgaagtg
      601 gccgccgagc acaaatatgt tcgtcccgtc gtctccgaga cgcccgtgct gcgcctcacg
      661 gcgggacgcc atccggtgat cgagcgaatg tttgccgagg gcgaattcgt cgccaacgat
      721 ctgaaactcg gcggcgaaaa cgcgccggag atagctttga tcacagggcc gaacatgtcc
      781 ggcaaatcca cttacctgcg gcaggccgcg ctcatcgtcc tgatggcgca ggccgggtct
      841 ttcgtccccg cgacgtatgc caccatcgga ctctgcgaca gaatcttcac gcgcatcggc
      901 ctctatgatc gtctgggtag cggcgaatca acttttatga cagagatggt ggagacggcg
      961 cagatactcc accatgcctc cgagcgttcg ctcattctgc tcgacgaact agggcgcggc
     1021 acttccacct atgacggcct cgccgtcgcg cgcgccgtgc tcgaatacat acacaaccat
     1081 ccgcaactac gcgcgaagac actcttcgcc acgcactatc acgaactaac cgcgctcgcc
     1141 tccgtcctcc cgcgcctcga aaatctacac gtcgagatcg cggaggaggg aagcgaactt
     1201 gtcttcctct acaagatcag ccccggcagc gcggagaaaa gttacggcgt ctatgcggcc
     1261 aaactcgcag ggctgccgcg ccctgtggta cgacgcgccg aagagttgct gcgcgaatac
     1321 gaatcacagg gcggcgagag acattttccc ggcgagagcg cggccaaaca gacggcgcaa
     1381 cttccggcgc gagaacgcga gtccaaaatt attcaggcgt tgatgtcact cgatcccgac
     1441 gcgctcgctc cggttgaagc cttgatgaaa cttttcgagt tgcgccgcat ggcggaagag
     1501 tgtgtggatg agacggggaa agtaagggcg attcgcagcg cgtaaccgga caggctcgtt
     1561 cggaaggggc agagaatgtc agaagtccca agtctcaagt cataaaggcg cgtttgactt
     1621 gaggcctggg acttctcact tattaagccg gatcggtgtg aatctgaact ttggcgcgcg
     1681 ggatttcggc ttcgatggtg cgcagcacta tgacggaagc ggcgtgcgct tcttcgagag
     1741 tcttgtcgcg ttctacttcc agatgcaggt cgataaattt ctgcgacccg gagcggcgcg
     1801 tgcgcagatc gtggaaaccg agcactccct cgccgcgaaa gcgacccacg atctccgcca
     1861 cctttttatc cacttccggc ggcagacgcc gatccatcaa cgcgtccacc gactcgcgcc
     1921 ccacgctcaa ggccgaagcc agaatgtaga tcgagatggc gatggagatg agcgggtcaa
     1981 cgatgttcca gccggtgaaa tagattaaga cgagtcctgc gagcacgccg ccgttcgtcc
     2041 acacgtcgga agcaaaatgg actgcatctg cactcaaagc gggcgagtct gtgagttccg
     2101 ccgcacgtcg caggcgcgcc acgagcgcga ggctcacgac tatcgcgatc agcatcgtcg
     2161 ccacgccgat tgattcctgt tcggtcggtt gcggatggat cactcgcctg atcgcttcga
     2221 agattaagaa cagcccggag agcgtgatca cagccgcctg aaaaagtccg gccagcgact
     2281 cggccttgcc atgcccgtaa gcatgatctt cgtcgggcgg gcgagaggcg gcgcgcacgg
     2341 cgaaatagtt gatggtggag gcgagaatgt ccatcgtcga atcgagcagc gaagcccaga
     2401 cggctatcga gcctgtcaac cagccggttc cggtcttgag caggatcaaa aaggctgcgg
     2461 cgaggatgga gagccgcgcc gtagagttct tgagggctga ctctctggca cggacgcgcg
     2521 cgtgcgtttc tctcgcagtt ggaactgaca tcgaagtcga acgtgaaacc ggctgcgccg
     2581 agcatctcac agagacatta gcgcaacggg catcttaaca aattcaatga ccgagcgctt
     2641 ccgggcgttc ggagacttct tcaagcaccc gactccgcga gcgcagcgtg acggttgcca
     2701 gtcccgcgaa gatcaaggcg gcggcaagcc acgtccgcga gttcaactgc tctccgagaa
     2761 tcagcggcgc gacggcgaac gcgatgagcg gttgcaggta aatgtagatg gcgacgatac
     2821 tcggcgcgac gcgtcccaaa gcccacgcgt tcaggtaata tgccgcgacc gtagggacga
     2881 ggatgatgta gagcatcgcg caccatgcga gcaccgggac gctcgacaaa gaaaattttg
     2941 ataagtggta gccacctatt ggcagcgtga tgaaacttcc gaaaatgaag atccaggtga
     3001 tgaccgtcag cgcgccgtag cgtttgatca tgtcctgtga gatggcgatg tatgcgccat
     3061 aagccatcga gttgagcacg aggataaaat tccctatgtt cgtctcgcgg gaaaagctgg
     3121 cttccgcagg attgatgaga tagacgacgc cgcacgcggc cagaaagagt ccgacggcga
     3181 cgcgccacga gccgctttca tagccgagga aaacgctcac gaggagtgtg aaaataggga
     3241 tcgtcgagcc gattaaagcg gagttgataa cggtggagag ttcgagtcct ttgacgtaga
     3301 ggaattgatt gagaaccact ccgagcaggc tgtacagcag gagccgcgca aagtcggctc
     3361 tccccttgat gctcacatga ttggtggcgc ggcggatcac cagaaaggcg agcgtcgcgc
     3421 cgacgatgcg aaaagtgacg agtccggtgc tggacagcga acgcagtacg agtttcccga
     3481 cgataggcca cgtgccgaaa atgagctgca cggcgatgag cgcgccgtgc ggtttgaagc
     3541 tctttgactg aaccggcgca gtcgtagtaa cggtggtggc gttcatcaaa agagcgttcg
     3601 accccttgct cgtcaacaga cacgacaggc gcggaaactc acaacatgag catcaacggg
     3661 ttttccagaa tctttttgaa agtttgcaga aacttcgcgc ccgtcgcgcc gtcaacgatg
     3721 cggtgatcgc aggacatcgt gacgcgcatc atctggcgca cggccatctc gccgtcgcgc
     3781 acgaccggct tctggctcat cgcgccgacc gccagaatcg cggcctccgg cggattgatg
     3841 atcgccgtaa actcttcgat gccgaacatg ccgaggttgc tgaccgaaaa agtcgcgccc
     3901 atgtattctt cgggctggag tttacgcgtg cgtgcgcgtt cggccagttc acgcacttca
     3961 cggctgatct gcccgataga cttgcgctcc gctccgcgca cgatgggcgt gatcaatcct
     4021 tcatcaatgg cgacggccac gccgatgtcc gcgtgctcgt aataacggat cgttttatcc
     4081 tgaaaggaag cgttgacctg cgggtgctgc atgagcgcga cggcagccac tttgatgatg
     4141 atgtcgttga tgctgacctt gttctgaggg tcgagcgcgt tgagcgaggc gcgtagctcg
     4201 gcggtgcgat ccatctcgat ctcggtggtc aggaagaagt gcggcacggg accgatggac
     4261 gaggcgagac gccgcgcaat cgtgctgcgc atggtcgaga cgggttcgtc tcgataaggc
     4321 gatgcccctt cgaccccagg tagctgcgcg ggttgttgcg gcgcgcgcgc ttctgcggcg
     4381 cgaagttcgg tcggctcggc ttgcgtgcgt ccctgcgcgt tgtcagccgt ggcttgctgc
     4441 tgtccggcat tgatggcggc ttcgatgtcg cgcttgatga tgcgaccact gggtccgctc
     4501 cctgtgagcg tcttgaggtt gatgttggct tcggcagcca tgcgcgcggc gatgggcgag
     4561 acgacgatgc gcccgcccgc ccgttgaccg ttgccggaag cgttgtcgcc gccgggagac
     4621 gcggcctccg gtgtcgaagt ttgagcgggc ggaggctccg gcgcgttgcc ggttgaaggc
     4681 gcgccgcctg cttcaccctt gatcggttcg cccgcaatgg tggcctgcgt gcgcgctccc
     4741 ccgacgacaa ctccggccaa ctctctctgc gcgccgtcct gcccggcttc gacgctctcc
     4801 tcgatgctct tcttattggc agcggcggcc tcgcccgact cggcctgcga gacgagcgcg
     4861 gagatgtctt cgtccgcttc gccgatgatg gcgatgacct gtccgagcgg cacggtcgct
     4921 ccggcctcga cgagaatttt gcgcagcacg cccgtcgtca acgcttgcgc ttccatcgtc
     4981 gccttgtcgg tatcaatctc ggcgatcggt tcgccgacgc tcaccttgtc gccttcattc
     5041 ttcaaccagc gcgccagttg tccctcttcc atcgtcggcg agagcttcgg catgactacc
     5101 tgcgttgcca taaatttttc ctcgtgaata gtaaatcgta gatcgtaaat agaaagaaga
     5161 cagtctttat ctattttcga tctacgattc acgatttact aatcaatgta agcgacttcg
     5221 cgcacggcgg cgacgatgcg tgcgacatcc ggcagagcga gttgctcaag attcttggcg
     5281 tagggcatcg gggcatccgc gcttgagagc cgcttgacgg gggcgtccag ataatcgaaa
     5341 gcgcgctcca tgatctgcgc gctgatctcc gcgccgacgc cgcagaaagg atgcgattct
     5401 tcgacgatca cgacgcgatt cgttttcttc accgagttga tgatcgtctc gatgtcgagg
     5461 gggcgaatcg tgcgcgggtc aaccacttcg acgctcacgc cgtctttctc caactcctcc
     5521 gccgccttca acgcgagcgg aactgtcagc gagcgtgcga cgatagtggc gtccttgccc
     5581 tcgcgcttga tgtctgcgac gccgagcggg atcgtgaaat cgggatcgtc cggcacctcg
     5641 cccttcaagc catacatgcg ctcctgctcc atgaagatga ccggatcgtc gtcgcggatc
     5701 gcgctcttca agagtccctt cgcatcagcc gccgtcgaag gcatgaccac cttgaggccg
     5761 gggaagtgcg cgtaccacga ttcgagcgcc tgtgagtgtt gcgaggagac ttgataagcc
     5821 gagccattcg gaccacgaaa gacgatgggg attttgaatt gcccgcccga catgtagagc
     5881 atcttggcgg cggagttgat aatctgatct gtggcgagga tcgagaaatt gaaagtcata
     5941 aactcgatca cgggacgcag gccgaccatc gccgcgccca cgccaagccc cgcgaaaccg
     6001 agttccgtaa ttggggaatc gatcacgcgc ttctcgccga actcttccaa cagtcccttc
     6061 gaaactttat acgcgccctg atacgcggcg acttcttcgc cgagcaaaaa cacgttctcg
     6121 tcgcgatgca tctcttcgct aagcgcctga ttcaaagcct ctcgaatcgt aattacggcc
     6181 attcaacaaa cctccaaaga cagtgacagg tgacaggtga cgggtgacaa gtgacgagca
     6241 agaaataaga caagttctgt tgttttaact cgtcacttgt cacccctcac tcgtcacttt
     6301 tatcgggttg gcatagatat gcgtgaagag ttctgcgggc gcggggagcg ggctttcttc
     6361 cgcaaacttg acggagtgtt ccacctccgc gcgcacgcgg tcttccagtt ctttgagcgc
     6421 ggcatcgtct atgacgccct tgtgtctgag actgtcggag aaaagtttga cggggtcgcg
     6481 ttcctgatgc ttttcgatct cggcgcgcgt gcggtagttg ccggggtcgg acatggagtg
     6541 acccatgaag cgataggtgc gcacctcgat gagcgtcggg agcgactctt tgcgtgcgcg
     6601 ttcgacagcg cgctctgtgg cctcgcggac ggcgagcacg tccatgccgt caacaaactc
     6661 cgaggccatg ccgtaagcgt gcgctttctg cgaaacgtct tgcagagaca tcgcgcgggc
     6721 gagcgaagtg cccatgccgt actggttgtt ttcgcagaag tagatgcagg gaagtttcca
     6781 gagctgcgcc atgttgaaag attcgtgcag cgcgccctga ttgacggcgg cctccccgaa
     6841 gaagacgagc gaaacctgac ccgtgccctt gtacttcgca gcgaaggcga cgccggtggc
     6901 gagcggaatc tggccgccga cgatgccgtg gccgccgaga aaataggcgt ccgagtcgaa
     6961 gaggtgcatc gagccgcctt tgcccgccga gcagccgccc gctttgccgt agagttcggc
     7021 catcacttcg tcagccgaag tgccgcgcgc cagcgcgtgg ccgtgatcgc gatagctcgt
     7081 catcacgtaa tcgtccgggc ggatcgccga gagcgcgccg acggcgactg cttcctgtcc
     7141 gatgtagaga tgacagaagc cgccgatctt gccgagcgtg taggcttccg ccgccttctc
     7201 ttcgaaacgc cggatgagca ccatctggta gagcatgttt cgcagcgtct cccgatcgat
     7261 ctcttcgatc tgctggtgct ggtgctgctg gcgttgctca tgctcctcgc gcagttctgc
     7321 ggggttgatc gtgtccgtgc tgtcgaggtc ggcgatgcct gcgacggctg cctgacccgt
     7381 ggattgatcg cgtggtttca acggtgtcac tttaagttcg ttcatgaaag tattctctca
     7441 gacctggcat ttgcgggtcg tgtgcgacac gaagacagcg cgcctgaagc ggctgcttcg
     7501 ctccccgacc caaaccactg ctctatgcgt ttgatagcaa tcagggatta tacgcgagat
     7561 gccagccgct gcgcaaaagc aacaaaaaga gccggaaaag acgcaaaacg gcgcatcctg
     7621 aactaaattc aggatgcgcc gtgaagttgt gaaagatgcg cgcctgttat ctgaaagagt
     7681 cgagcgagag caccatgttc tgcgggtcgg acggaggatt gagcttgcgc ccgtgtttgt
     7741 cttgctgcat gttgcgcccg gcctgtgaga ggagcgattc ggaggtttcg ccgtcggtcg
     7801 ggaaacaggc gatgccgatg ctgatgccga tgcgtgtggt gcgcccggtg cgaatcgtga
     7861 actcgtgcga ctcgaccgca gcacggatac ggtcggccac catctcggcc acgtcgcccg
     7921 aagccatcgg cataattgcg acgaactcgt cgcccgcgta acgcgcgagg atgtccatct
     7981 gccggaactg ttttttcacg atggcggcca cgcttgccag caggcggtcg ccaatcgcat
     8041 gtccgtgcgt gtcgttgatt tgtttgaaat tgtcgaggtc catgctcaag acggagagcg
     8101 gttcgcggtt gagccgctgg cactctgcga tacgctgttc cagcgcgaga tggaaggagc
     8161 gcgcgttggg aaggtctgtc agcgtgtcgg tgagcgcgct ctctttggtc ttctcaaacg
     8221 tcaacgcatt gttgagcgcg atggaagcgt gctgcgaaac cgattcgagc aggcggacgt
     8281 gctcggtcgt gtaagtagca cgcgtctttg aatagacagt gattgcgccg aacgctccgt
     8341 cttcacgaat cagaggggag acgagcacgc cgcgaatgtt gctggaaact tcttcgggca
     8401 ggccgacgag atcgagttcg ggcgacgtgt tgcacatcga acgagcgttg gcgataaccc
     8461 acccggagat gccttcgcca atcgtgatgc ggcgacgcgc gaaagcctcc gcgtgctcgc
     8521 ccgaagcgtg cgcggcgacg gctttgcccg agcggtcgtc aacgagataa atgatgcagg
     8581 tgtcgaacgg aacaatcgag cgcagtttgc tgctgacgag cgtaaccgtg tcctgcaagt
     8641 tgagcgacga gccgatagtt tgagcgatct cgtggagggc gaacacctcg cgctgtgctt
     8701 cggcgatgga gcggaaaccc gaatcgccat ccgaggcgga gccgagaatg tccgacgcca
     8761 gacccgcatc gggcgcgccg ttcaacttgg catcgtcttc ggaggccacc tgctcctgaa
     8821 tgtcgtgctc ggcgatgaga ttgtcgaagt agtctatgtt ggagacaaac atgtccacga
     8881 cgcgcgggtc gaaggaagtg ctcgccatgc gacgcagcag cgcgagcgaa tcttcgcgtt
     8941 tcatgccgac gcgatagggg cggtcgcagc gcgtggcgtc gtagaaatcg accaccgaga
     9001 tgatgcgcgc gacgagcgga atctgttcgg cgcgcaagcc cttcggataa cctgaaccgt
     9061 cccacttctc atgatggtag cggacgatgt cttccacggg gtaagggaag ttgacgcgct
     9121 tgatgatgtc gccgccgacc accgtgtgaa tcttcatctt gtcgaattcg gcggcggtca
     9181 gtttgccggg cttgttcaag atgtattccg gcacggccag tttgcccacg tcgtgcagga
     9241 gcgcgcccgc gcgcagggcg tgcaactcct gttcgccgac gccgaggatg cgtccgagtt
     9301 ccatcgcgta aatctgcgtg cggcggacgt gcccgtgagt cgtctgatcc ttcgcatcaa
     9361 tggcgatggc gagcgactcg atggtgttca tgtggaggtc tgcgatctcc tgtatgtgcc
     9421 ggagtttcgc ttcttcgccc ctccgcgtct cgctcacgcg cttctcgtcg aaacggaaca
     9481 gcaggtagac cagcgaaacg atcagcacgc cgacaaacag gaacgacatg ccggagccga
     9541 tgaaactcga atagaacaga gccgccgccg ctgcgcccgc caactgcgtc atcgaagtcc
     9601 agacgaggct ctcacgcgaa aactccaact tctttgtccc tgcttcgtag gccacgtaga
     9661 aagccgacag cagagtgttt agtccgaatt gcacgatggc gaggacgaag agcgggaaga
     9721 tcaacctttc cagcggcgcg ttatgcgctt ctgcggaggt tgcgccgccg atggtcagac
     9781 gcgtgagaag gttgtagacc atgacggcga tgcaggtaga aatgacggca gccgcggtgc
     9841 gaaagaccag accggcgcgg tcgcccgttt ttctcgaaga catgaagccg tcagccccgg
     9901 ccagaatcgc agccggagcc gtgccgaaca tcatcgccgc caaaaagatc agcgcgtcgg
     9961 ccacagactt gctgccgtgg ctcttgccgt cggcgttgac gaggggaacg ttgagaagac
    10021 tggcggcgaa ggttaaggcc aacaacgcgc cgcaccagag atagccgtta gtcgtgagcg
    10081 acagcagacc gtcgagagag aagacgagga gggctgccgc tagcgctgcc gtagcgcata
    10141 aaagcgcgag gctcttccag tttgcgttgg gcataattag ttggagttag cgagaacgtt
    10201 ggaaaaactg ttagttttgg agtttgcagg aagcctgcga catgtctgta catgaagcgg
    10261 gaagaacgcg gggtaaaaac ttgccaaaca acgttggcac atacaaaata accgctttta
    10321 agcgattcgt caaggatttt tcacagaaaa tggaggataa acaggcggac gc
//
LOCUS       DASWNS010000422        24304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_170599, whole genome shotgun sequence.
ACCESSION   DASWNS010000422 DASWNS010000000
VERSION     DASWNS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 24304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24304
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170599"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VGB76_14630"
     CDS             <1..442
                     /locus_tag="VGB76_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100182.1"
                     /translation="ESLIKQSAFVNQVVVVGAGRKTPAALIVPDWDALQSALAENGEG
                     SSEEGAKGKKKPGKSTHAALSRNPAAVRLVQREVAALTASLHDYERVRRVLLLPEEFS
                     IDGGEMTPTLKVKRRVIDEKFGALIEELYEGAGGGAATAASKDE"
     gene            complement(585..908)
                     /locus_tag="VGB76_14635"
     CDS             complement(585..908)
                     /locus_tag="VGB76_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100183.1"
                     /translation="MGSISTANFCKHKACPSAEMLLGYTQATLGRELRQQMSAHLEAC
                     DFCDAEMYLLSKFPPVGVAVYPAVNIPRSLYRLAKDLLSVSTTAAGRAVEILYERERL
                     SLTDA"
     gene            1876..3084
                     /locus_tag="VGB76_14640"
     CDS             1876..3084
                     /locus_tag="VGB76_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100184.1"
                     /translation="MTSSTEEELHAPMHDDAELTAPAVATAVAERPSRGSNAQWVESL
                     RRSDVTRLWSELHRIVCSHPLVRASHSAGLLVEGAERGNAYVDLTQELFVTLLSKARF
                     QHYLETAMTDAEIECEISQIELTNLLTAELRKRHPESYRLARRISTIIQSSANFRRFD
                     GGSGSVEDEPHRRLADRVYGLSEWPAAKLRRNSQELEQRIQMIPVHQRDTRMVGCTGD
                     SQIIISNSDLEDLIISILEAADAPVDVRSLRSLVMSRLPVMDIYLVPLGGGDDEENGR
                     IFEPVDGRENPEQGLLRRESEQEAVGYVDRFLRGLHTSVRGKTKQYDRMLGVLWHCYL
                     SPDHVTQLEAASRLGVSDSLVSDYRRRIEQELRALAFGEIEQARRFETSLRERVRALV
                     LDSEEESVAV"
     gene            3309..4922
                     /locus_tag="VGB76_14645"
     CDS             3309..4922
                     /locus_tag="VGB76_14645"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0100185.1"
                     /translation="MQKKHPFTDYEAVKPSTRRTRAAGGEEAGDLWTVSDNSGEARRS
                     SVENPGSVERLERVATLENNDEQARTPHGEAAPHSEEARPAGANASPFTLKRGHLFSY
                     LGLFLFTVVLYFRPYELHPSLAGLTSLAYWLALATLAVFLPTQLALEGNLTTRPREVN
                     LVLLLCVAALLSIPFAIDPGEAWGTFNYIFIKAVIMFIVIVNVVRTPLRLKGMIFLGL
                     AIGCVLSVGAINDYRLGRFVVGDERVAGVVGGMFGNPNDLALHLVTMIPLAVGLLLST
                     RNLLAKPFYALCALLMVGGVIASFSRGAFIGLLGASMVLAWKLGRRNRFLMIILIGVF
                     LVALLALAPGEYTERISSIGSVSRDASSSSRRDLLVRSIIVAIANPVFGVGMGNFHIV
                     SIRELVSHNAYTQVAAEMGLAAMCIYIMMLVVPLKRLRLIEHATYETRRGSRIYYLSV
                     ALQASLVAYMMSSFFGSVAYQFYVYYLIGYAVALRRIYEAQTGMEVVPLSKKEKRAAR
                     AAAAVSPQNSDTDFPEPESPHSLALRTGM"
     gene            4966..6096
                     /locus_tag="VGB76_14650"
     CDS             4966..6096
                     /locus_tag="VGB76_14650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0100186.1"
                     /translation="MLEDQPTTNLHSARRSAVARAGGVAPPQPALHAPAELPFVSIVI
                     PCYNEERFIYKVLENLAGQYASARSEIIVVDGRSTDRTRERVAEFKRAYPSLRVRLVE
                     NPARNIPAGVNLGIEEARGDIIVRMDAHSIPSSNYVRCCVEQLEGGAKGEEAASVVGM
                     PWRIQPGAETREARAIALAVAHPFGIGDAKYRMPERAATEFVDTVPFGVFRKSLWREV
                     GGFNEALLANEDYDFHYRIRQRGGRILLDTSGHSLYFARPTIRELARQYFRYGTWKAQ
                     MVKLHPRSLRLRHLVAPAFVAGVALTSLAGFWWRPAWWLLLLAVVPYALLALVCAFGL
                     ARRGRDLTLLPLIAVIFPVLHVAWGSSFWLGLVRAPRGARTK"
     gene            6121..7086
                     /locus_tag="VGB76_14655"
     CDS             6121..7086
                     /locus_tag="VGB76_14655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0100187.1"
                     /translation="MNWQRKAQIQNLIASLPMADAFYYAMQRSIGSLRKGRANPVEYF
                     GAARRVAEWIRTTGRDVEGMRFLEVGTGRNVNLPTALWLCGAAQVTTVDLNSYLSSAI
                     VFESNEYARQHRAEVLQTFGAEAERPGFQARLAGLLDYKGDLAGLLKMMNVEYLPRAD
                     ASRLPVASESFDAHVSFAVFEHIPAEVLAAILAEARRLLRPDGLLVHIIDPSDHFSHD
                     DASITAINFLQFDDAEWAALAGNKFMYHNRLRAFEYLELFARAGVRVLEQQQTLDEPS
                     LRSLKNGFPLHPQFRQIAAEELAVRSLNVLGTFAAMEAGEAAEAH"
     gene            7136..8683
                     /locus_tag="VGB76_14660"
     CDS             7136..8683
                     /locus_tag="VGB76_14660"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEY0100188.1"
                     /translation="MVEVERRKESSLTMRAAWIMSARTVAFVLSFALPLLLVRRLDQT
                     AFGQYKQVFLVVGTAVNVLPLGFHMSAFYFLPREPERRGEVVFNILLFHLLVAAAACL
                     AFVLRPALLASLFPGSGELVAYAPALGLVLLLWVVSSFLEAIAMANQETKTATIFIVS
                     MQLARTLLLLVAGVVFASVESLIYAAIIQGALQTIVLFVYLFLRFENFGRRFDWRLLR
                     TQLSYALPLGIAGFLYSMQMDMHNYFVSNRFDAATFAIYAIGCFNLPLIHILSDSVGS
                     VMIPRVAELEKQGARREIVVLISRMMRKLAAAYFALYAFLIVAGQEFIVVLFREQYLA
                     SWPIFAVNLAMIPLGLIASAYDPVMRAYPEHRFFLLRVRAVLLGALFVALWFGTGRVG
                     LVGVISLVVLTNLLERIVVAVRCGKALGMTWGQLALFKDVGKLACAAACAALATVLVR
                     AALVEAMPPFFVLAVCGVVFACVYLAGVWGFGILSADERVGAGRALRTLQRRTTHWRR
                     ATNDVAG"
     gene            8700..10130
                     /locus_tag="VGB76_14665"
     CDS             8700..10130
                     /locus_tag="VGB76_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100189.1"
                     /translation="MAGQTVHDLLSRKLLLPLTRAQRLARASRRPAMRAFYAGMRFRR
                     ESAAWDLEERRAWMLKRLRHVVRRAAAETLFYRELFRRERFDPQADFSFDDFARLPTL
                     EREEIRQAGRALISNAVPADQLRKDSTGGSTGEPTHVWLGPEELGWRESALEYPMQRI
                     GAPTGTPTGYLWGHHLDPVARETWRERYHDFESNVRWFDCFRLSAEVLAGYHAEFTRW
                     RPRCIIAYASALAALAEFVLSENLKPNYPTKCFVTGAEKLLPAQRAAIERAFPARSLH
                     ERYGSRDVGIMGFQIEPARTHDFEVDWQNLLVEPETTEADSPILITKLHADAMPMLRY
                     RVGDVGRFPRASAQPGHPAFVLHEVLGREADRIWLPDGRWITGLQMPHMLKDYPVREF
                     MFLQRPDYSVELQVIPHNNFGEDSRRSILETVEANLPGLRVSLALVQSIPRTKANKLR
                     PVVSEVKRTTGNDVSEAREVSEGNGR"
     gene            10127..11605
                     /locus_tag="VGB76_14670"
     CDS             10127..11605
                     /locus_tag="VGB76_14670"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEY0100190.1"
                     /translation="MTELLRRKMSCENARVGVAKTGAPRYPSGERAATSGANEISSAL
                     ARLARLLGWGDEESGAFSKLIPRGARVLVKPNFVMHANQCADGIEPLVTHESLVRAVV
                     EALLQTEAAEVSIGDAPVQGCDFAALLRETKLGEWSDELRRRESRFRGIRDFRRTIAV
                     FRDGVRVASENLRDESEFTLFDLGRESLLEPITDESGAFRVTCYDPRLMQKTHAPGRH
                     QYLVARAVLDADVVINLPKLKTHKKAGITCALKNLIGINGNKEFLPHHRIGGARAGGD
                     CYPGANPLKRSLEFVLDRQNSTARPALGKVWQGLGLQLERAARLAGDDLGVEGSWSGN
                     DTVWRTALDLNRVMLYGQADASFSDEPARLVVHIADAVVAGQGDGPLAPEALPLGLLL
                     GASNAAAMDWVGAYLLGYEPQRVSIAREAFARFRWPLTTFTHEEVSVCGDLGEGAADE
                     LLGRRPEPLFRVKHPAGWRDAAAAATVASNGAPAPARSQTRS"
     gene            11698..13728
                     /gene="asnB"
                     /locus_tag="VGB76_14675"
     CDS             11698..13728
                     /gene="asnB"
                     /locus_tag="VGB76_14675"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY0100191.1"
                     /translation="MCGIAGLVIAQDSRAAAGDDAVARMIRSLARRGPDGEGVERWPH
                     GSALGHRRLAIFDLSEAGRQPMLSKDGTVGLVFNGAIYNFRELRAELEARGCRFVSQT
                     DTEVLVEGYREWGIDALVGRLHGMFAFGLWDERAQKLFLVRDRLGVKPLVYASAPGGR
                     LAFASNVRALRAAGMTGEIDAEAMVEYLEFGFVTDGRSIYQGATKVPAATIVEWSAPE
                     ATTQARTYWSPPVALEPDASHAPKFEEVVEEVERLFLRAVEMRLFADVPVGALLSGGV
                     DSSLVCWAIAELGGDVTAYTIGTPGDPLDETADARATAATLGISHRVLDLSANDAPDV
                     SELAAAYAEPFACSSALGMLRVSRAVAPSATVLLTGDGGDDVFLGYPEHRYLWLASRL
                     ARHTPGAAASLWSNTLRTRFPRRGLARRAASFLDYTTGGLGAVTNARDGLPVYEQHAL
                     LGERLAGARLAQRNIPRSRESGRNVLAEFLEYDRRTRFTGEYLPKVDGATMHYALEAR
                     SPFLDQKLWEYAAALPLDLRLKGGTLKAVLRELARRRLGERVARGRKRGFGIPVGRWL
                     AGRWRERVEETLRDSSLGREGWINAGNALRQFGFEVERGEASLQTWYLFVLETWMRHE
                     RSANVFEPPAGDVERLTMATLKAREVSGVEVGVQGIPAEAATTTVSEDSHAA"
     gene            13856..15079
                     /locus_tag="VGB76_14680"
     CDS             13856..15079
                     /locus_tag="VGB76_14680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0100192.1"
                     /translation="MKPNVLLFIDSFAQGGTERQVVQLARLLSESGRYQIHLACLSGE
                     GVLRAEAERLGFTDIPEFPLNSFYDRNMVVQLRRCAAFLRERAIDIVETHDFYTNIFG
                     MTAAALARTPRARIASRRETDGTKTRKQKWVQQRAFTLADAIVANAEAVRQELIRDGV
                     PPAKIQTIYNGMDTARVAPRPDLRRDDALALLGLPREEDDGRRRRFVSIVANMRHAKK
                     DQATFLRAARRVREAVPEAAFVLAGEGELREPLIALASELGLADSAFFTGRCAHVPEL
                     LAVSEACVLSSSGTEGFSNSIIEYMAAARPVVATDIGGAREAIIEGETGYVVAPGDDA
                     ALASRLISLLTDADAARRMGERGLQVVKEKFSCAAQVEHVENLYERLLAKKRAAPQPS
                     PRTNADAGARHESAG"
     gene            15140..16330
                     /locus_tag="VGB76_14685"
     CDS             15140..16330
                     /locus_tag="VGB76_14685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0100193.1"
                     /translation="MERAHEKIDVTNTKATRQGRRAVRVLIVAPSLGILGGQAVQAAR
                     LLARFQTEPSLEVSFLPINPRLPGLLGKLQGVKYVRTVLTSVAYIASLLARVRGFDVI
                     HIFSASYSSFVIAPTPAILVAKLYGKKIVLNYRSGEAEDHLTRWRRTAIPTIRLVDEV
                     AVPSGYLVDVFARFNLHARSIYNFVETDRFRFRRRKPLRPVFLSNRNLEPLYNVGCIL
                     RAFAIVQRRFPAAALTVAGDGSQRAELEQLARELGLRNTEFIGRVEQARMHELCERAD
                     IYLNSSNIDNMPGSIIEAYAAGLPVVTTDAGGIPYILTHEETGLMVGRGDQEALAASA
                     IRLLEDAELAERLASNALAACRRYSWAAVRDEWLKLYFELAHEDAAVSPSNVPTQVGS
                     NTNA"
     gene            16380..19004
                     /locus_tag="VGB76_14690"
     CDS             16380..19004
                     /locus_tag="VGB76_14690"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEY0100194.1"
                     /translation="MIKVLRKLKGRSLTELRVRGVQAAACLAERAGVSAATRLPAGAS
                     FFALLDASRVGSDAGDARLTSESLHAHFRTRPAQAFFAAFDAPDATRAALATRFGGEA
                     RAALVERAERIVAGRFDLLGLRELDFGDPVDWHLEPVAGKRTPASAHWSRIDYLDASV
                     AGDKKITWELNRQQYFMVLGRAYWHTNDERYAETFARHLAGWMDANPPKRGINWASSL
                     EVAFRAISWLWSLQFFKHSPHLDAALYTRALKFLHAHARHLETNLSTYFSPNTHLTGE
                     ALALVYLGTLLPEFRAASRWRETGARILHAELHRQVRPDGVYFEQTSYYQRYTADFYL
                     HFRALLGTGHNATTHAAPLEEKLQALLDHLMYITAPDGTTPLFGDDDGGRLAPFDERA
                     PNDFRATLATGATLFRRPDYKFVAREATEETLWLLGAQGLQDFDALHAEQPARQSRAF
                     TDGGYYVMRDGWTSDNSYLLLDCGTHGTLGGAHAHADALAILGCTARGFNALVDSGTY
                     TYTGSPALRDEFRATAAHNTLNIDGESSSVPESAFRWRRMADAACHKWLSHARFDLFE
                     GEHDGYMRLDAPAKHLRSVLFIKGDYWIMRDRVETGGAHRYELNFHFNPRARVSIETA
                     NDAGAAAAANDAGAAETANDGGAETAADDSRAERAASVVRARESSSRASSALDLFAFA
                     RGGAWRAQDKLISAAYGAATPAPACTFAFSGDAAEAVTLLVPRAASGQPYPANAEPYL
                     AGAEPYLTGAARYSAREIEAVGGRAFELRNEGAGARARDFVLMRAGGAHAVETARVVS
                     DFEWMWLRFDGDEAEPRELIAIGGRTLRLDGREVLQTRERVNYLVARQREGDAPLVET
                     DASEDLFLALFNKVTSDR"
     gene            19098..21086
                     /gene="asnB"
                     /locus_tag="VGB76_14695"
     CDS             19098..21086
                     /gene="asnB"
                     /locus_tag="VGB76_14695"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY0100195.1"
                     /translation="MCGINGIAFSERAREGRVEASDLVRMRDVIEHRGPDDSGMMLDG
                     AVGLGHRRLSIVDVSAGHQPMTNEDDTLYIVYNGEIYNHAEHRPALEARGHRYRTHCD
                     TETILHLYEEHGAACVERLRGMFTFAIWDARRRELFIARDRLGVKPLYYVHRADGSLF
                     FASEIKSLLAARAVRAEINHASLPDYLANHGTSGEETLFAGVKRLLPGHTMLWRADGT
                     LKIEKYWDLHFAEDAADDGAEISARGRRSDADYIAEWSELFRKSVELRLMADVPLGMF
                     LSGGIDSSAIAAVMSGMVSDPIKTFSVAFKEREANELYYARLVAEKFRTEHHETIVSP
                     EDFFGALPRLVWHEDEPLAHPSSVALYFVSRLAAQHVKVVLTGEGSDEMLGGYERYYK
                     TIYNLALAPGYQRLTGASVRRAVKRRIESLPLDSKVRHKLRRTFLCLPPDIESLYFDN
                     FAVFTRAMQNDLLTPEARERAGGIEPYRDMRRYFETNRSRSLLNRMLYADTKTYLHEL
                     LMKQDQMSMAASIESRVPFLDHKLVEFTARLPQHLKLRRGWTTKYILRQSMKDVLPAP
                     ILSRKKMGFPVPVGAWFRGAYRGIVEEYVLGERAAARNLFDRAYVRELVARHNAGENH
                     TERLWSLVNVEIWLRQFMDGEAASLPGETKNEVVSAVA"
     gene            21132..22391
                     /locus_tag="VGB76_14700"
     CDS             21132..22391
                     /locus_tag="VGB76_14700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0100196.1"
                     /translation="MRILWLKTELLHPVDKGGKIRTYQMLKELKREHHVTYLTLDDGS
                     GAPDAAKRASEYCHELVRVPHRTRAKFSAGFYGELATNLVSPLPYFMKKYQSAEMRRE
                     IERLVATDEFDVLVCDFLQPSVNVPPRLPIASVLFQHNVEAMIWKRHYEVQKNLLKKS
                     YLYGQWLKADAYERAACRRFDTVVAVSVEDRETMRRAYNLQSVSDVPTGVDTEFFRPR
                     GEEPREPHNLVFTGSMDWLPNEDAIQFFTKEIMPRIKERVPGATLTVVGRNPYASLIE
                     LGKRDPSIIVTGRVEDVRPYMERAAAYIVPIRVGGGTRLKIYEAMAMEKPIISTTIGA
                     EGLPVREGQELLLADTAEAFASAVVRVLTDEELARSLGERAAVAVREKFSWDKVSRAF
                     AEACQLAVAARAAKQPVSTFKQVNQTP"
     gene            22442..23959
                     /locus_tag="VGB76_14705"
     CDS             22442..23959
                     /locus_tag="VGB76_14705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0100197.1"
                     /translation="MSKKPYHLLLLLVTLVYLALAVGSARTKSPWSDEAWFAQAGLNL
                     ATRGEMTTPVLETTGTNFKGLDRHTYWIMPLHLVTQAGWYKVFGFSLLSMRVLSVVFG
                     VLALFSWYFIVGNLSGNQKIALLAFVLLAFDYIFVMAASFGRGDMMSAALGAAGLAAY
                     LSLRERNFNWAILASQSLIAACGLTHPNGGVAFFFGLLFLTLYFDRTRLNWRHLGLAA
                     IPYLLGALGWGAYILQAPSDFMAQFTANASTGGRMSGLTSPLSAIKNEITLRYLTAFG
                     LGAHTSGSSGPIWLKSLVLLAYIVALAGCLSVRSIRAHKGYRALLILTGIFFLVLTFF
                     DGQKLSFYLVHIVPLYTALLAVWIYWCYSKRFVPRWLLALCVCGLLAIQLGGLLYRMK
                     LNVYQKSYLAATSFIAAHAKEDSMVMGSAVLGFELGYERIVDDVRFGFRSGKRPDFIV
                     INDVYEETIRYYRAGGEGAELAAHVNNLLNEEYELVYDRNFYQIYARRNFMTRKS"
     gene            23991..>24304
                     /locus_tag="VGB76_14710"
     CDS             23991..>24304
                     /locus_tag="VGB76_14710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0100198.1"
                     /translation="MAAIKDYAKATTAAHTRASQPVIAVLIPCYNEELTVAEVVADFR
                     AQLPEADIYVFDNNSTDATVERAREAGAQVFYERRQGKGYVVQSMFRQVDADIYIMVD
                     GDG"
BASE COUNT         4393 a         7574 c         7702 g         4635 t
ORIGIN      
        1 cgagagtttg atcaagcaga gcgcgttcgt caatcaagtc gtcgtcgtcg gcgcgggacg
       61 caaaactccc gctgcgctca tcgtccccga ttgggatgca ctgcaaagcg cgctcgcgga
      121 aaacggcgaa ggctcgtcgg aagagggcgc gaagggaaag aagaagccgg gcaagagcac
      181 acacgccgcc ctcagccgca acccggctgc cgtgcgcctc gtccaacgcg aggtggccgc
      241 cctgaccgct tctttgcacg actacgaacg cgtgcgtcgc gtcctgttgc tgcccgaaga
      301 attttcgatt gacggcggag agatgacgcc gactctgaaa gtcaaacggc gcgtgattga
      361 tgaaaagttc ggcgcgttga ttgaagaact ttacgagggc gcaggcggcg gagccgcgac
      421 agcagcctca aaagatgagt aggggcaaga gagactgctg tttgaaattg tggggagagc
      481 ggggagaagc gagagcttga ggggcgaaga gagccgggaa gcgaagttgg caaaaagcgt
      541 gctgccggtt tgaacgcgtg cggacaggtt ccgcgtgtgc ggcttcaggc gtcggtcaac
      601 gagagcctct ctctttcata gagaatctcc acggcgcgtc cggcggcagt ggtggagacg
      661 gagagtaaat ctttggcgag ccggtagagc gagcgcggaa tattgaccgc cggatacacg
      721 gccacgccga cgggcggaaa tttcgagagc aggtacattt ccgcgtcgca gaagtcgcaa
      781 gcctctaggt gcgccgacat ctgctggcgc agttcgcgcc cgagcgtcgc ctgcgtgtag
      841 ccgagcagca tctcggcgga cgggcaggct ttgtgtttgc aaaaattggc tgtgctgata
      901 gaacccatgg ttccgctcct tctacatcgt cgaccggagc ctccggttcg gacttcacag
      961 tccgactctt ttgacgtagg ctcttagtcg aagcgcgccg cacgtttcaa aaggcacagc
     1021 atgaacaggc cgagggcaaa tagctgacga ggtgagaggc acagccgtag tacccttgtc
     1081 aattgggcgc ttcgcttagt ctctacagag aaatcgctaa tggcggcaaa gttccataac
     1141 tgaggtgggg tgaaagtgcg atttcttcct ttttttcaac gtcttggggg gagtgtaaaa
     1201 agatgttgct caaacagcgg ctaactgtta gtattatttt gtcgtcggga aacgcgatag
     1261 tttccctgac tccccttcgt ggctgaccgc gcctgttccc cttagcggtc atctggcacg
     1321 gtctatccag attcagcact acccaaccga gcatgcgggt tgctactctt ttcggtctct
     1381 ccccgaaaaa ttggtaagca cgagccttcg attgcgcgcg caatctttcg ggcgcgcgtg
     1441 gcatttaaga cggaagcctt caaagtaagt gtccggttcg ttctccaagc ctgagaggaa
     1501 agttgcttat tgcgtttcgc gcccctgcaa tagataatga gcgacaattc gattcagggt
     1561 gaacggagaa cggaagcagg tggagctttg agactttggc ttttgcccgc ccggcaggct
     1621 cttggttggt agggacggcg actcggggag aggacacgga ctgcgaaagt ttgacatgcc
     1681 aggtgttcgt tgagccttca acgcgcacgc agtcaaccag agaggaacga gtttaacgac
     1741 atccagcctc ttgtggaaaa cttgcctaaa gcagacaagc ctcaatagta cccgcccggg
     1801 aaattagcct gatagacact acttaataac atggctgcaa catctgccga tcataatcac
     1861 ccattcgata atcccatgac cagctccacc gaagaagagt tgcatgcgcc gatgcatgac
     1921 gacgctgagt taacggctcc ggctgttgcc actgccgtcg ccgagcgccc ctcgcgcgga
     1981 agcaacgccc aatgggttga atccctgcgc cgctccgatg tgacccgcct ctggtcggag
     2041 ttgcaccgca tcgtctgtag ccacccgctc gtgcgcgcct cgcacagcgc aggtctgctc
     2101 gtcgaaggcg cggagcgcgg gaacgcctac gtcgatttga cgcaggaact tttcgtcacg
     2161 ctgctcagca aggcgcgctt ccagcactac ctggagacgg cgatgaccga cgccgagatc
     2221 gaatgtgaaa tcagccagat cgagttgacg aatctgttga ctgccgaact gcgcaaacgt
     2281 catcccgaat cctatcgcct cgcacgccgc atctccacca tcatccaatc gagcgcaaac
     2341 ttccgtcgct tcgacggcgg ctccggcagc gtcgaagacg aaccgcaccg caggctcgcg
     2401 gatcgcgtct acggtctgag cgaatggcct gccgccaagc tgcgccgcaa ctcgcaggag
     2461 ttggagcaac gcattcagat gatccccgtc caccagcgcg acacgcgcat ggtcggctgc
     2521 acgggcgact cgcagatcat catcagcaat tccgacctcg aagacctgat tatctcgatc
     2581 ctcgaagcgg cggacgcgcc cgttgacgtg cgttccttgc gcagcctcgt catgtcgcgc
     2641 ctgcccgtga tggacattta cctcgtgccg ctcggcggcg gcgacgatga agagaacggc
     2701 agaattttcg agccggtgga cgggcgcgag aatcccgaac agggcttgct caggcgcgag
     2761 tcggagcaag aggcggtcgg gtatgtagac cgtttcctgc gcggcctgca cacctctgtg
     2821 cgcggcaaga cgaaacagta tgaccggatg ctcggcgtgc tctggcattg ttatctcagc
     2881 cccgaccatg tgacgcagct tgaagccgcc tcgcggctcg gcgtctccga ttcgctcgtc
     2941 tccgattacc ggcggcgcat cgagcaggag ttgcgtgcgc tggccttcgg cgagatcgaa
     3001 caggcgcgac gcttcgagac ttctctgcgc gaacgcgtgc gcgcgctggt gctcgatagc
     3061 gaggaagaga gcgtggcagt ttaagttgcg ggatggtcgg gtcgggtttg aacctctcac
     3121 ccggcgagtg taaaagcggt gtcacgtgtt ttatcgtgcg ccgcttcgtt ttcatagtag
     3181 ctcggaagtt ttaacgcgca gagggcaggg cagccgaacg aacgcgaacg ccgacggact
     3241 aatattgaag ttgttcaagt taatctgagg tcttctgaag cgcgcggctt cggtcgttaa
     3301 attgtaagat gcaaaagaag catcccttca ccgattatga agcggtcaaa ccgtccacgc
     3361 gccgcacgcg cgccgcagga ggcgaggagg cgggtgacct ctggacggtg agcgacaact
     3421 ccggcgaagc gcgccggtcg agcgtcgaga atccgggaag cgtcgagaga ctcgaaagag
     3481 tggcgacgct tgaaaacaac gacgagcagg caagaacgcc gcacggcgaa gcagcgccac
     3541 acagcgaaga ggcgcgcccc gccggggcga acgcctcgcc tttcacactc aagcgcgggc
     3601 acctgttctc ttatctcggt ctttttcttt tcacggtcgt cctctatttt cgcccttacg
     3661 aactccaccc gtcgctcgcc ggtttaactt cgctggccta ctggctcgcg ctggcgacgc
     3721 tcgcagtttt cctccccacg caactcgcgc tcgaaggcaa tctcacgacg cgcccgcgcg
     3781 aagtcaatct cgtgctgctg ctgtgcgtcg ccgcgctgct ctcgattccc ttcgccatcg
     3841 atccgggcga agcctggggc accttcaact acatcttcat caaggccgtc atcatgttca
     3901 tcgtgatcgt gaacgtggtg cggacgcccc tgcgtctgaa gggcatgatc tttctcggcc
     3961 tcgccatcgg ctgcgtcctg agcgtcggcg cgatcaacga ttaccggctc ggtcgtttcg
     4021 tcgtcggtga cgagcgcgtg gcgggcgtcg tcggcggcat gttcggcaat ccgaacgacc
     4081 tcgcgcttca tctcgtgacg atgatcccgc tcgccgtcgg cctgctcctg agcacgcgca
     4141 acctgctcgc caagcccttc tacgccctgt gcgccctctt gatggtcggc ggcgtgatcg
     4201 cctcgttttc acgcggcgca ttcatcggac tcttgggcgc aagcatggtg ctcgcgtgga
     4261 aactcggacg ccgcaaccgc ttcctgatga tcatcctgat cggggtgttt ctcgttgcgc
     4321 tcctcgcgct cgcgccgggc gagtacaccg aacgcatcag cagcatcggc tcggttagcc
     4381 gcgacgcgtc gagttcgtcg cgccgcgatc tgctggtgcg ctcgatcatc gtcgccatcg
     4441 ccaacccagt gttcggcgtc gggatgggca acttccacat cgtctcgatc cgcgaactcg
     4501 tcagccacaa cgcttatacg caggtcgccg ccgagatggg gctggcggca atgtgcatct
     4561 acatcatgat gctcgtcgtt cccctcaagc ggctgcgcct gatcgaacac gcaacttacg
     4621 aaacgcgccg tggctcgcgc atctattacc tgtccgtcgc gttgcaggcg agtctggtcg
     4681 cttacatgat gagcagcttc tttggctctg tggcctatca attttatgtc tattacctca
     4741 tcggctacgc cgtcgctctg cgccgcatct acgaagcgca gacgggcatg gaagtagttc
     4801 cactctctaa aaaagagaag cgcgccgctc gcgccgccgc cgcagtatcg ccgcaaaact
     4861 ccgacacaga cttccccgaa cccgaaagcc ctcactcgct cgccttgaga acggggatgt
     4921 gagacgggct acgcccgcgc gcggagcgtt tcacgctgaa aatcaatgct cgaagatcaa
     4981 ccgacaacga atctgcacag cgcgcgccgc tccgccgtcg cacgcgccgg aggcgtcgcg
     5041 ccgccacagc ctgcgctgca cgcgcccgcc gaattgcctt tcgtctccat cgtcattccc
     5101 tgttacaacg aagaacgctt catctacaaa gttctcgaaa acctcgccgg tcaatacgcc
     5161 tccgcgcgct ctgaaataat cgtcgttgac gggcgctcga ccgaccggac gcgcgagcgc
     5221 gtggccgagt tcaagcgtgc ctatccgagc ctgcgcgtgc ggctcgtgga gaacccggcg
     5281 cgcaacattc cggcgggcgt caatctgggc atcgaggaag cgcgcggcga catcatcgtg
     5341 cgcatggacg cacactcgat cccctcctca aactacgtgc gctgctgcgt cgaacagttg
     5401 gagggcggtg cgaagggaga ggaggcggca tcggtcgtcg ggatgccgtg gcgtattcag
     5461 ccgggggcgg agacgcgcga ggcgcgggct atcgctctcg ccgtcgcgca cccgttcggc
     5521 atcggcgacg cgaaatatcg catgcctgaa cgcgccgcga cggagtttgt agacactgtg
     5581 ccgttcggag ttttccgcaa gagcctgtgg cgcgaagtcg gcggcttcaa cgaagcgttg
     5641 ctggcaaacg aggactacga ttttcactat cgcatacgcc aacgcggcgg gcgcatcctg
     5701 ctcgacactt ccggccactc gctctatttc gcgcgcccga ccatacgcga actcgcgcgc
     5761 cagtatttcc gctacggaac gtggaaagcg cagatggtaa agctccaccc gcgttctctg
     5821 cggctgcgac atctggttgc gcccgccttc gtcgcgggcg tcgccttaac gtctctcgcc
     5881 ggtttctggt ggcgtccggc gtggtggctg ctcttgctcg ccgtcgtgcc ttacgcgttg
     5941 ctcgcgctcg tctgcgcgtt cgggctggcg cgtcgcgggc gcgacctgac tttgctcccg
     6001 ctcatcgccg tcatcttccc cgtgcttcat gtcgcgtggg ggagcagttt ctggctgggc
     6061 ttggtgcgtg cgccgcgcgg cgcgaggacg aagtagcagg ggatgtaaca gggaacggcg
     6121 atgaactggc aacgcaaagc acaaatccaa aacttgatcg cttcgctgcc gatggcagac
     6181 gcgttctact acgcgatgca gagaagcatc gggagcttac gcaaagggcg cgccaacccc
     6241 gtggaatatt tcggcgcggc gcggcgtgtg gccgaatgga tcagaacgac cgggcgcgac
     6301 gttgaaggca tgcgctttct cgaagtcggc acggggcgca acgtgaacct gccgaccgcc
     6361 ctctggctct gcggcgcagc gcaggtgacg accgttgatt tgaattcgta tctatcgagc
     6421 gcaatcgttt ttgagtcgaa cgaatacgcg cggcagcatc gagcggaagt cttgcagacg
     6481 ttcggcgcgg aggcggagcg tccggggttt caggcacggc tcgccgggtt gctcgactac
     6541 aagggcgacc tcgccggact cttgaagatg atgaacgtag agtatttgcc gcgcgccgac
     6601 gcgtcgcgtc tgccggtcgc ttccgaaagt ttcgacgcac acgtttcgtt cgcggtcttc
     6661 gagcacatcc cggcagaggt gctcgcggcg attctcgcgg aggcgcgccg cctcctgcgc
     6721 cccgatggcc tgctcgtcca catcatcgat ccttcggatc atttctcgca cgacgacgct
     6781 tcgatcacgg cgatcaactt tttgcagttc gacgatgcgg agtgggcggc tctggcgggc
     6841 aacaaattta tgtatcacaa ccgcctgcgc gctttcgagt atctggaact gttcgcgcgc
     6901 gcgggcgtgc gcgtgctcga acagcagcag acgctcgacg agccttcgct gcgctcgctc
     6961 aaaaacggct ttccgctgca tcctcagttc agacagatcg cggcggagga gttggccgtc
     7021 agaagcctca acgtgctcgg gacgtttgcg gcaatggagg cgggagaggc cgcagaggca
     7081 cattgaaagc tgtcaggctg ttatcatgta aacgggaagg gaagcggtaa aagcgttggt
     7141 tgaagttgaa cgccggaaag agagttcgtt gacgatgcgt gcggcatgga ttatgtcggc
     7201 gcgcacggtg gcgttcgttc tgagcttcgc gctgccgctg ctgctcgtgc gccgtctcga
     7261 ccagacggct ttcgggcagt acaaacaggt cttcctcgtc gtcggcacgg cggtcaacgt
     7321 gctgccgctc ggctttcaca tgagcgcgtt ctactttctg ccgcgcgaac ccgaacggcg
     7381 cggcgaagtg gtcttcaaca tactgctctt tcatctgctc gtcgccgccg ccgcgtgtct
     7441 cgccttcgtc ctgcgcccgg cgctgctcgc gagcctcttc cccggttcgg gcgaactcgt
     7501 cgcctacgcg cccgcgctcg gcctcgtgct gttgctgtgg gtcgtctctt cgtttctgga
     7561 agccatcgcg atggcgaatc aggagacaaa gacggcgacc atctttatcg tctcgatgca
     7621 actggcgcga accctgctcc tgctcgtggc aggcgtcgtg ttcgcttcgg tcgagtcgct
     7681 gatctacgca gcgatcatac agggcgcgct gcaaaccatc gtgctcttcg tctatctctt
     7741 cctgcgcttc gagaatttcg ggcgacgctt cgattggcgt ctgctccgca cgcagttgag
     7801 ctacgcgctg ccgctcggta tcgccggatt tctctattcg atgcagatgg acatgcacaa
     7861 ctatttcgtg tccaaccgct tcgacgccgc caccttcgcc atctacgcca tcggctgctt
     7921 caaccttccg ctcatacaca tcctcagcga ctcggtcggc tccgtgatga tcccgcgcgt
     7981 ggccgaactg gagaagcagg gcgcgcgacg cgagatcgtc gtgctcatct cgcggatgat
     8041 gcgcaaactc gccgccgctt atttcgccct ctacgccttt ttgatcgtcg cggggcagga
     8101 gttcatcgtc gtcctgtttc gtgaacaata tctggcgagc tggcctatct tcgccgtcaa
     8161 tctggcgatg atcccgctcg gcctgatcgc ctcggcctac gatcctgtga tgcgcgctta
     8221 cccggagcat cgcttcttcc tgctgcgcgt gcgcgcagtg ttgctcggcg cgttgttcgt
     8281 cgcgctctgg ttcggcacgg ggcgcgtggg tttggtcggc gtcatctcgc tcgtcgtgtt
     8341 gacgaacctg ttggaacgca tcgtggtcgc ggttcgctgc ggcaaagctc tggggatgac
     8401 ttgggggcag ctcgctttgt tcaaagacgt ggggaaactg gcctgcgccg ccgcgtgcgc
     8461 tgcgctggcg accgtgctcg tgcgcgctgc gctggttgaa gcgatgccgc cgtttttcgt
     8521 gctcgccgtc tgcggcgtgg tcttcgcgtg cgtctatctg gcgggcgtgt gggggttcgg
     8581 gattctgtct gcggatgaac gcgtgggcgc aggtcgcgcg ctgcgcacgc tgcaacgccg
     8641 cacgacacac tggcgacgcg cgacaaacga tgtcgccgga tgaaaggaac ttgagcgttt
     8701 tggccggaca gaccgtacac gatctgttat cgagaaagtt gctgctgccg ctgacgcggg
     8761 cgcagaggct ggcgcgtgcg tcgcgtcgtc cggcgatgcg cgccttctac gcggggatgc
     8821 gctttcgtcg cgagtcagcc gcgtgggact tggaggaacg gcgcgcatgg atgctcaagc
     8881 gtctgcgtca tgtcgtgcgc cgcgccgccg ccgagacgct cttttatcga gagcttttcc
     8941 gacgtgagcg attcgacccg caagccgatt tcagcttcga cgacttcgcc cgtctcccca
     9001 cgctcgaacg cgaagagata cgacaggccg gacgcgcgct catctcgaac gccgtccccg
     9061 cagatcaact gcgcaaagat tcgacgggcg gctcgaccgg cgagccgacg cacgtgtggc
     9121 ttggacccga agagttgggc tggcgcgaga gcgcgctgga atacccgatg caaaggatcg
     9181 gtgcgccgac cggcacgccg acgggttatc tctgggggca tcatctcgac cccgtggcgc
     9241 gcgaaacgtg gcgcgaacgc tatcacgatt tcgagtcgaa cgtccgctgg ttcgattgct
     9301 tccgtctgtc tgccgaggtg ctcgcgggct atcacgcgga gttcacgcgc tggcgtccgc
     9361 gctgcatcat cgcttacgcg agcgcgctcg ccgcgctggc cgagttcgtg ctgtcggaaa
     9421 acctcaaacc aaactacccg acgaaatgtt tcgtcacggg cgcggagaaa cttctccccg
     9481 cgcagcgcgc cgctatcgaa cgcgccttcc ccgcgcgctc cctgcacgaa cgctacggca
     9541 gccgcgatgt cggcatcatg ggctttcaga tcgagcctgc gcgcacgcac gattttgaag
     9601 ttgactggca gaacctgctc gtcgaaccgg agacgacaga ggcagactct cccattctga
     9661 tcacgaaact gcatgccgat gcgatgccga tgcttcgtta tcgcgtgggc gacgtggggc
     9721 gtttcccgcg cgcgagcgcg cagccggggc atccggcgtt tgtcctgcac gaagttttgg
     9781 ggcgcgaagc ggatcgcatc tggctgccgg acgggcgttg gatcacgggg ctgcaaatgc
     9841 cgcacatgct caaagattac cccgtgcgcg agttcatgtt tctgcaacgc cccgactact
     9901 cggtcgagct tcaagtgatc ccgcacaaca atttcggtga agactcgcgg cgaagtattc
     9961 tggagacggt tgaggcgaac ctgcccggcc tgcgcgtcag cctcgcgctg gtgcagagca
    10021 tcccgcgcac gaaggccaac aaactcagac ccgtggtgag cgaggtcaaa cgcaccacag
    10081 gaaacgacgt aagcgaagca agggaagtaa gcgaagggaa cggacgatga cggagttgct
    10141 gcgccggaag atgtcttgtg agaatgcgcg cgtcggcgtg gcgaagacgg gtgcgccgcg
    10201 ctacccgtcc ggcgaacgcg cggccacttc cggcgcgaat gaaatctcct ccgcgctcgc
    10261 acgcctcgcg cgcctgctcg gctggggcga cgaagagagc ggcgcgttct ccaaactcat
    10321 tccgcgcggc gcgcgcgtgc tcgtcaagcc gaacttcgtg atgcacgcga atcagtgcgc
    10381 ggacggcatc gagcctctgg tcacgcatga gtcgctcgta cgcgccgtcg tcgaggcgtt
    10441 gttgcagacg gaggcggcgg aagtgtcaat cggcgacgcg cccgtgcagg gctgcgactt
    10501 cgcggcgttg ctgcgcgaga cgaaactcgg agaatggtcg gatgaattga ggaggcgcga
    10561 aagtcgcttt cgcgggatac gcgatttccg ccggacgatt gccgtctttc gagacggcgt
    10621 gcgcgtcgcg tcggaaaatc tgcgcgacga gtcggagttc acgctcttcg atctcggtcg
    10681 cgagagcttg ctcgaaccga tcacggacga gagcggcgcg tttcgcgtga cgtgctacga
    10741 cccgcgtttg atgcagaaga cgcacgctcc cggcaggcat caatacctcg tcgcgcgcgc
    10801 cgtgctcgat gcggatgtgg ttatcaacct gccgaagttg aagacgcaca agaaggccgg
    10861 gatcacctgc gcgctcaaga atctgatcgg catcaacggc aacaaggagt ttctgccgca
    10921 tcatcgcatc ggcggcgcgc gtgcgggcgg cgattgctac cccggcgcaa accctctgaa
    10981 gcgttcgctc gaattcgtgc tcgaccgcca gaactctacg gcgcgtcccg cgctcggcaa
    11041 agtctggcag ggcttgggtc tgcaactcga acgcgcggcg cggctcgcgg gcgacgatct
    11101 gggcgtggag ggttcgtggt cgggcaacga cacggtctgg cgcaccgcgc tcgatctcaa
    11161 ccgcgtgatg ttgtacgggc aggcggacgc gagcttctcg gacgaaccgg cgcggctcgt
    11221 cgtgcatatc gcggacgcgg tggtggcggg gcagggcgac gggccgctcg cgcccgaagc
    11281 gttgccgctc ggtctgctct tgggtgcatc gaacgctgcg gcgatggatt gggtcggcgc
    11341 gtatctgctc ggctacgagc cgcagcgcgt gtcgattgcg cgcgaggcgt tcgcgcgttt
    11401 tcgttggccg ctcacgacct tcacgcacga agaagtgagc gtgtgcggag acttgggcga
    11461 aggcgcggcg gacgaactgc tcggcagacg cccggagccg ctctttcgcg tcaagcatcc
    11521 ggcgggctgg cgcgacgctg ccgctgccgc gacagtcgct tcaaacggcg cacctgcgcc
    11581 ggcgcgaagt cagacccgaa gttgattctc gcgcacagtt aattctcaca gcagttaatt
    11641 cctgcgcgct tgcagttaat tcttgaagga gactctgctc cgaattttgt taagaccatg
    11701 tgcggaattg ccggattagt gatagctcaa gacagtcgcg cggcggcggg cgacgatgcg
    11761 gtcgcgcgga tgattcgctc gctcgcgcgg cgtggtcctg acggcgaagg ggtcgagcgt
    11821 tggccgcatg gcagcgcgct cggccacagg cggctcgcca tcttcgacct gagcgaagcc
    11881 gggcgacagc cgatgctctc gaaggacggc acggtcggcc tcgtcttcaa cggcgcgatc
    11941 tacaacttcc gcgagttgcg cgcggaactc gaagcgcgcg gttgccgctt tgtcagccag
    12001 acggatacgg aagtgctcgt cgaaggctat agggaatggg gcattgatgc gctcgtcggg
    12061 cgtctgcacg gcatgttcgc cttcgggctg tgggacgaac gcgcgcagaa actttttctc
    12121 gtgcgcgacc gcctcggcgt caagccgctc gtctacgcga gcgcgcccgg tggccgcctc
    12181 gccttcgcct cgaacgtgcg cgcgcttcgt gccgcaggga tgacgggcga gattgacgcg
    12241 gaggcgatgg tcgaatatct cgaattcggt tttgtcaccg acgggcgttc gatctatcag
    12301 ggcgcgacga aagttccggc ggccaccatc gtcgaatggt ctgcgccgga agcgacgacg
    12361 caggcgcgca cttactggtc gccgcccgtc gcgctcgaac cggatgcgtc gcacgcgccg
    12421 aagttcgagg aggttgtcga agaggttgag cgtctgtttt tgcgcgcggt cgagatgcgg
    12481 ctctttgcgg acgtgccggt cggcgcgctc ctgtcgggcg gcgtggattc gagcctcgtc
    12541 tgctgggcga ttgccgaact cggcggcgac gtgacggcct acaccatcgg cacacccggc
    12601 gacccgctcg acgagacggc ggacgcacgc gccaccgccg caacgctcgg catctctcat
    12661 cgcgtcctcg atctttcggc gaacgacgcg cccgatgtct cggagttggc ggcggcctac
    12721 gccgagccgt tcgcgtgttc gtccgcgctc ggcatgctcc gcgtgtcgcg cgccgtcgcg
    12781 ccttcggcca cggtcttgct cacgggcgac ggcggcgacg acgtgtttct aggctacccc
    12841 gaacatcgct acctctggct cgcctcgcgc ctcgcgcgcc acacgcccgg cgcggcggcg
    12901 agcctctggt cgaacacctt gcgcacgcgc tttccgcgtc gcggcctcgc gcgtcgcgcc
    12961 gcctcttttc tcgactacac gaccggcgga ctcggcgcgg tgacgaacgc acgcgacggc
    13021 ctgcccgtct acgaacagca cgcgctactc ggcgaacggc tcgcgggcgc aaggctcgcg
    13081 caacgcaaca tcccgcgctc gcgcgaatcc gggcgcaacg tgctcgcgga gtttttggag
    13141 tacgaccggc gcacgcgctt cacgggcgaa tacctgccga aggttgacgg cgcgacgatg
    13201 cattacgcgc tcgaagcgcg ctcgcctttc ctcgatcaga agttgtggga atacgccgcc
    13261 gcgctcccgc tcgatctgcg tctcaaaggc ggcactctga aagcggtctt gcgggaactg
    13321 gcgcggcgtc ggctcggcga gcgcgtggcg cgcgggcgca agcgcggctt cggcatcccg
    13381 gtcggacgct ggctggcggg gcgttggcgc gagcgcgtgg aagagacgct gcgcgattcc
    13441 agcttggggc gcgaaggatg gatcaacgcc ggaaacgcgc tccggcaatt cggctttgag
    13501 gtcgagcggg gcgaagcctc tttgcagacg tggtatctct tcgtgctcga aacgtggatg
    13561 aggcacgagc ggagcgcgaa cgtcttcgag ccgccagcgg gcgacgtgga gcgtttgacg
    13621 atggcgactc tcaaggcgcg cgaagtgagc ggcgtggagg tcggcgtgca gggaattccg
    13681 gcggaagccg cgacgacgac ggtatcggaa gatagtcatg ccgcatgaga aggtggaggc
    13741 ttgaaggcaa gcgtaactga ggaagtaact gaggaagtag ctgaggaagt gtcgccgccg
    13801 ggcgcaactt ttagcgtcga gcgattgacg agtttacgaa agcgttttta atgtgatgaa
    13861 accgaacgtc cttctcttca tagacagctt cgcgcagggc ggaaccgagc ggcaggtggt
    13921 gcaactcgca cgcctgctct cggagagcgg gcgttatcaa attcatctcg cctgcctgag
    13981 cggcgagggc gtgctgcgcg ccgaagccga gcgtctgggc ttcacagaca ttcccgaatt
    14041 tccgctcaac tccttttatg atcgcaacat ggtcgtgcaa ctgcgtcgct gcgccgcgtt
    14101 ccttcgtgaa cgcgccatcg acatcgtcga gacgcacgac ttctacacca acatcttcgg
    14161 catgaccgcc gccgcgctcg cgcgcacgcc ccgcgcacgc atcgcctcgc gccgcgaaac
    14221 cgacggcact aagacgcgca agcagaagtg ggtgcagcaa cgcgccttca cgctcgccga
    14281 tgcgattgtc gccaacgctg aagcggtcag gcaggaactg atcagagacg gcgtgccgcc
    14341 cgcgaaaatt cagaccatct acaacggcat ggataccgcg cgcgtcgcgc cgcgtcccga
    14401 cctgcggcgc gacgatgcgc tcgctctcct tggcctgcca cgcgaagaag atgacggacg
    14461 ccgccgtcgt ttcgtgagca tcgtcgccaa catgcgacac gccaaaaaag atcaggcgac
    14521 gtttttgcgc gcggcgcgac gcgtgcgcga ggccgtgccg gaagccgcct tcgtgctcgc
    14581 gggcgagggc gagttgcggg agcctctcat cgcgctggcc tctgaactcg gacttgcgga
    14641 ttcggctttc ttcacggggc gatgcgcgca cgtgccggag ttgctcgccg tctccgaggc
    14701 gtgcgttctc agttcgagcg ggacggaagg tttttctaat tcgatcatcg agtacatggc
    14761 cgccgcgcgc ccggtggtgg cgacggacat cggcggcgcg cgcgaagcca tcatcgaagg
    14821 agagacgggc tacgtcgtcg cgccgggcga cgacgccgcg ctcgcgtcgc gtctcatctc
    14881 gctgctcacc gatgcggacg ccgcgcggcg gatgggcgag cgcggcttgc aggtggtgaa
    14941 agaaaagttc tcctgcgcgg cgcaggtcga gcacgtcgag aatctatacg agcgcctgct
    15001 cgcaaagaaa cgcgccgcgc cgcagccgag tccgcgcacg aatgcggacg cgggcgcgcg
    15061 acacgaaagc gcaggataga caatagacaa aggcgaaccg taacaggcag gccgaggcat
    15121 ggaacgagcg cacagaggca tggaacgagc acacgaaaaa atcgacgtga cgaacacgaa
    15181 ggcgacgcgg caaggacggc gcgcggtgcg tgttttgatc gtcgcgccgt cgctcggcat
    15241 actcggcggt caggccgtgc aggccgcgcg gctgctcgcg cgttttcaaa cggaaccgtc
    15301 gctcgaagtt tcgttcctgc cgatcaaccc gcgcctgccc ggcctgctcg gcaagttgca
    15361 gggcgtcaag tacgtgcgca ccgtgctgac ttcggtggcc tacatcgcat cgctccttgc
    15421 gcgcgtgcgc ggcttcgacg tgatacacat cttctccgct tcctattcgt cattcgttat
    15481 cgcgccgacg cccgctatcc tcgtagccaa actctacggc aaaaagatcg tcctcaatta
    15541 ccggagcggc gaagcggaag atcatctcac gcgttggcga cgcacggcga ttcccacgat
    15601 tcgtctggtg gacgaagtgg ccgtgccttc cggttatctc gtagacgtgt ttgcgcgctt
    15661 caacctgcac gcgcgttcga tctacaactt cgtcgagacg gatcgttttc gttttcgtcg
    15721 ccgcaagccc ttgcgccccg tcttcctctc gaaccgcaat ctcgaaccgc tctacaacgt
    15781 cggctgcatc ctgcgcgcgt tcgccatcgt ccagcggcgt ttccccgcag cggctttaac
    15841 cgtcgcgggc gacggcagcc agcgcgccga actcgaacaa ctcgcgcgcg aactcggact
    15901 gcgcaacacg gagttcatcg ggcgcgttga gcaggcgcgg atgcatgagc tttgcgagcg
    15961 cgcggacatc tatctgaaca gttccaacat agacaacatg cccggctcga tcatcgaggc
    16021 ttacgcggcg ggcttgcccg tcgtgacgac ggacgcgggc ggcatccctt acatcctgac
    16081 acacgaagag acgggcttga tggtcgggcg cggcgaccag gaggcgttgg cggcctccgc
    16141 gatccgcctg ttggaagacg cagagttggc cgagaggctc gcgtcgaacg cgctcgccgc
    16201 gtgccgccgg tacagttggg ctgcggtgcg cgacgaatgg ttgaaactct atttcgagtt
    16261 ggcgcatgaa gacgctgctg tcagcccgtc aaacgtgcct acgcaggtcg ggagcaacac
    16321 gaacgcttga gaggtttgga gagacgagta gagagagagt cgctgcgacg tgaagttgaa
    16381 tgattaaagt tctgcgaaaa ctgaaaggcc ggagtttgac ggagttgcgc gtgcgcggcg
    16441 tgcaggctgc cgcgtgcctc gcagagcgcg cgggagtttc ggcggcgacg cgcctgcccg
    16501 ccggtgcctc gttcttcgcg ctgctcgacg cctcgcgcgt cggcagcgac gcgggcgacg
    16561 cacggctcac ttccgaatcg ctgcacgcac attttcgcac gcgccccgct caagcgttct
    16621 tcgctgcctt cgacgcgccg gacgcgacgc gcgccgcgct cgccacgcgc ttcggcggcg
    16681 aggcgcgtgc ggctctcgtc gagcgcgccg aacgcatcgt cgccggtcgc ttcgacctgc
    16741 tcggcctgcg cgaactcgac ttcggcgatc cggttgactg gcatctcgaa cccgtcgcgg
    16801 gcaagcgcac gcccgcgagc gcgcactgga gtcgcataga ttatctcgac gcgtcggtgg
    16861 cgggcgataa gaagatcacc tgggagttga accgtcaaca gtatttcatg gtgctcggtc
    16921 gggcttactg gcacacgaac gacgaacgct acgccgagac tttcgcgcgc cacttagcgg
    16981 gctggatgga cgccaacccg ccgaagcgcg gcatcaattg ggcgagcagt ctggaagtcg
    17041 ctttccgcgc gatctcgtgg ctgtggtcgc tacagttctt caaacattcg ccgcatctcg
    17101 acgccgcgct ctacacgcgc gccctgaaat ttctacacgc ccacgcgcgc catctcgaaa
    17161 caaatctctc aacctacttc agccccaaca cgcacctgac gggcgaggcg ctcgcccttg
    17221 tctatctcgg cacgctgctg ccggagtttc gcgcggcctc gcgctggcgt gagacgggcg
    17281 cgcgcattct ccacgccgaa ctccacaggc aagttcgccc cgacggcgtc tacttcgaac
    17341 agacgagcta ctatcaacgc tacaccgcag acttctatct acacttccgc gcgctgctcg
    17401 gaaccggcca caacgccacg acgcacgccg cgcccctcga agagaagcta caggctttgc
    17461 tcgatcacct catgtacatc actgcgcccg acgggacgac gcctctcttc ggcgacgacg
    17521 acggcggacg cctcgccccc ttcgacgaac gcgccccgaa cgactttcgc gcaacgctcg
    17581 ccacgggcgc gaccctcttt cgccgccctg actacaagtt cgtcgcacgc gaggcgaccg
    17641 aagagacgct ctggctgctc ggcgcgcagg gcttacaaga cttcgacgcg ctccacgcgg
    17701 aacagcccgc ccgacagtcg cgcgctttca cagatggcgg ttactacgtg atgcgcgacg
    17761 gttggacttc cgataacagt tatctcttgc tcgactgcgg cacgcacggc acgctcggcg
    17821 gcgcgcacgc acacgccgac gcgctcgcca tcctcggctg caccgcgcga ggcttcaacg
    17881 cgctcgtgga ttccggcact tacacctaca ccggctcgcc cgcgctccgc gacgaattcc
    17941 gcgcgacggc tgcgcacaac acgctcaaca tcgacggcga atcctcgtcc gtccccgaaa
    18001 gcgcgtttcg ctggcggcgg atggccgacg ccgcgtgcca taaatggcta agccacgcgc
    18061 gcttcgatct cttcgaaggc gagcacgacg gctacatgcg tctcgacgca cccgcgaagc
    18121 acctgcgcag cgtcctcttc atcaaaggcg actactggat catgcgcgac cgcgtggaaa
    18181 ccggcggcgc gcaccgttat gaactgaact ttcatttcaa cccgcgcgcc cgtgtctcaa
    18241 tcgaaacggc aaacgacgcc ggagcagcag cggcggcgaa cgacgccgga gccgcagaga
    18301 cggcgaacga tggcggagcg gagacggcag cggacgacag cagggcagag agggcggcga
    18361 gtgtcgtgcg cgcacgcgaa tcttcttcgc gtgcgtcgtc cgcgctcgat ctgttcgcct
    18421 tcgcgcgcgg cggcgcgtgg cgtgcgcagg acaaattgat ctcggcagct tacggcgcgg
    18481 ccacgcctgc gcccgcctgc acgttcgcct tttccggcga cgcggcagag gccgtcacgc
    18541 tcctcgtccc ccgcgcggca agcgggcagc catacccggc aaatgcggag ccatacctgg
    18601 caggcgcgga accatacctg acgggcgcgg cgcgatattc cgcgcgcgag attgaagccg
    18661 tcggcgggcg cgcattcgag ttgagaaacg agggggcggg cgcgcgggcg cgtgattttg
    18721 tgttgatgcg tgcgggcggc gcacatgcgg tcgaaaccgc gcgcgtcgtc tccgatttcg
    18781 agtggatgtg gctgcgcttc gacggcgacg aggcggagcc gcgagagttg atcgccatag
    18841 gcgggcgcac cctgcgtctc gacgggcggg aagttttaca gacgcgcgag cgcgtcaatt
    18901 atctcgtcgc gcgccaacgc gaaggcgacg cgccgctggt tgaaaccgat gcgagcgaag
    18961 atctcttcct cgcgctcttt aataaagtga cgagtgacag gtgacgagtg acaagttgaa
    19021 acccaactac ttgtctttca actcttcact tgtacccaac ctgtcacttg tcacctgtca
    19081 cctgtcactg ttctcttatg tgtggaatta acggaatcgc attttcggag cgggcgcgcg
    19141 aagggcgcgt ggaggcgtct gacctcgtgc ggatgcgcga tgtgatcgag catcgtggcc
    19201 ccgacgatag cgggatgatg ctcgacgggg cggtcggcct cggccatcgt cgcctgtcaa
    19261 tcgtggatgt cagcgcgggt catcagccga tgacgaatga agacgacacg ctctacatcg
    19321 tctacaacgg cgaaatctac aatcacgccg agcatcgccc cgcgctcgaa gcgcgcggcc
    19381 atcgctatcg cacacactgc gacacggaga cgatcctgca tctctacgaa gagcacgggg
    19441 cggcctgcgt cgagcgtctg cgcggcatgt tcaccttcgc catctgggac gcgcggcggc
    19501 gcgaactgtt catcgcgcgc gaccgcctcg gcgtcaagcc gctctactac gtgcatcgcg
    19561 ccgacggctc gctcttcttc gcctccgaga tcaaatcgct gctggcggcg cgcgccgtcc
    19621 gcgcggagat caaccacgcg tcgctgccgg actacctggc gaatcacggc acgtccggcg
    19681 aagagacgct gtttgccgga gtcaaacgcc tgctccccgg ccacaccatg ctgtggcgcg
    19741 cggacggcac cctcaagatc gaaaaatatt gggacctcca cttcgcggaa gatgcagccg
    19801 acgacggtgc ggaaatctcc gcgcgcgggc gtcggagcga tgccgactat atcgccgaat
    19861 ggtcggagtt gtttcgcaag tcggtcgagt tgcgcttgat ggccgatgtg ccgctcggca
    19921 tgtttctctc cggcggcatt gattcgtcgg ccatcgccgc cgtgatgagc ggaatggtga
    19981 gtgatccgat caagaccttc tccgtcgcct tcaaagagcg tgaagctaac gaactctatt
    20041 acgcgcggct cgtcgcggaa aaattcagga cggagcatca cgaaacaatc gtcagcccgg
    20101 aagatttttt cggcgcgctg ccgcgtctcg tctggcacga agacgaaccg ctcgcgcatc
    20161 cttcgagcgt tgcgctctat ttcgtttcgc gcctcgccgc gcagcacgtc aaggtggtct
    20221 tgacgggcga gggcagcgac gagatgctcg gcggttacga gcgttactac aagacgatct
    20281 ataaccttgc gctcgcgccc ggttatcaaa ggctgacggg cgcgagtgtg cgtcgtgcgg
    20341 tcaaacgccg catcgaaagc ctgccgctcg attcgaaagt gcggcacaaa ctcaggcgca
    20401 cgtttctctg tctgccgccc gacatcgaga gtctctactt cgacaacttc gccgtcttca
    20461 cgcgcgccat gcagaacgac ctgctgacgc cggaagcgcg cgaacgcgcc gggggcatcg
    20521 agccttatcg cgacatgcga cgctacttcg agacgaaccg ttcgcgctcg cttctcaacc
    20581 gcatgctcta cgccgatacg aaaacttacc tgcacgagct tttgatgaaa caggatcaga
    20641 tgagcatggc cgcttcgatt gagagccgcg tgccctttct cgatcacaaa ctcgtggagt
    20701 ttacggcgcg cttgccgcaa cacctgaaac tgcggcgcgg ctggacgacg aagtacatcc
    20761 tgcggcagag catgaaagac gtgctgcccg cgcccatcct ttcgcgcaag aagatgggct
    20821 tccccgtgcc cgtcggcgca tggtttcgcg gggcgtatcg cggcatcgtg gaagagtacg
    20881 tgctgggcga acgcgccgcc gcgcgcaatc tcttcgaccg cgcttacgtg cgcgaactcg
    20941 tggcgcgtca caatgcgggc gagaatcaca cggagcgtct gtggtcgctc gtcaacgtcg
    21001 aaatctggct gcggcagttt atggacggcg aggccgccag cctgccgggt gagacgaaga
    21061 acgaagtcgt ctccgccgtc gcctgaaact ccaaagaccg aaactcgcaa accgaaagtt
    21121 gaaagctaat cttgcgtatc ctctggctca aaaccgaatt gctgcacccc gtcgataagg
    21181 gcgggaagat tcgcacgtat cagatgctca aggagttgaa gcgcgagcat cacgtgacgt
    21241 atctgacgct cgacgacgga agcggcgcac ctgacgcggc aaagcgcgcg agcgagtatt
    21301 gccacgaact cgtccgcgtg ccgcaccgta cgcgcgcgaa gttctccgca ggcttttacg
    21361 gcgaactcgc cacgaacctc gtctcgccgc tgccctattt tatgaagaag taccaatcgg
    21421 cggagatgcg cagggagatc gagcggctcg tggcgacgga tgagttcgat gtgctcgtct
    21481 gcgattttct ccagccgagc gtgaacgtgc cgccgcgcct gcccatcgct tcggtgctct
    21541 ttcagcacaa cgtcgaggcg atgatctgga agcgacatta tgaagtgcag aagaacctcc
    21601 tcaagaaaag ttatctctac ggccaatggt tgaaagcaga cgcttatgaa cgcgcggcct
    21661 gtcgccgctt cgacacggtg gtcgccgtct ccgtggaaga ccgcgagacg atgcggcgcg
    21721 cttacaattt gcagtccgtg agcgacgtgc cgaccggagt ggacacggaa ttttttcgcc
    21781 cgcgcggcga ggagccacgc gagccgcaca atctggtctt caccggctcg atggattggc
    21841 tgccgaacga agacgcgatc cagttcttca cgaaagagat catgccgcgc atcaaggaac
    21901 gcgtccccgg tgcgacgctc acggtcgtcg gtcgcaaccc ttacgcgagc ctcatcgagt
    21961 tgggcaagcg cgatccctcg atcatcgtga cggggcgcgt cgaagacgtg cgcccgtaca
    22021 tggagcgcgc cgccgcttac atcgtgccga ttcgcgtggg cggcggcacg cgcctgaaaa
    22081 tttacgaagc gatggcgatg gagaagccga tcatctccac gaccatcggc gcggaaggct
    22141 tgcccgtgcg cgaagggcag gagttgctgc tggccgacac ggcggaagcg ttcgcgtcgg
    22201 cggtcgtccg cgtgttgacg gacgaagagt tggcgcgaag tttgggcgag cgcgcggcgg
    22261 tggcggtgcg cgagaaattc agttgggaca aggtgagccg tgcctttgcg gaggcgtgcc
    22321 aactcgccgt cgcggcgcgt gcggccaagc agcctgtctc aactttcaag caggtcaatc
    22381 agacaccctg agcgagggcg tgcgttaaga gatgcgcccg ccgtccccgg acattatatt
    22441 cgtgagcaaa aagccttatc acctgttgct gttactcgtc acgctggtct atctcgcgct
    22501 ggccgtcggg agcgcgcgga cgaaaagccc ctggagcgac gaggcgtggt tcgcgcaggc
    22561 gggcttgaat ctcgccacgc gcggcgagat gacgacgccc gtactggaaa caaccggcac
    22621 taacttcaaa gggctagacc ggcacactta ctggatcatg cccctgcatc tcgtgacgca
    22681 ggcgggctgg tataaagttt tcggattcag cctgctctcg atgcgcgtgc tgtcggtcgt
    22741 cttcggagtg ctggcgttgt tctcatggta cttcatcgtc ggaaatctct ccggcaatca
    22801 gaagatcgcg ctgctcgcct tcgtgctgct cgccttcgat tacatcttcg tgatggcggc
    22861 ctccttcggg cgcggcgaca tgatgagcgc cgcgctcggt gccgccggac tcgccgctta
    22921 cctctccctg cgcgaacgca atttcaattg ggcgatcctt gccagccaga gtttgatcgc
    22981 cgcctgcggg ctgacgcatc cgaacggcgg cgtggcgttt ttcttcggcc tcctgtttct
    23041 cactctctac tttgatcgca cgcgcctcaa ctggcgacat ctggggctgg ccgcgattcc
    23101 ttaccttctc ggcgcgctcg gctggggcgc gtacatcctg caagcgcctt ccgatttcat
    23161 ggcgcagttc acggcaaacg cttcgacggg cgggcgtatg agcggcctga cctcgccctt
    23221 gagcgcgata aaaaatgaga tcactctgcg gtatctgacg gcgttcggcc tgggcgcgca
    23281 cacttcgggt tcgtcgggac ccatctggct gaagagcctc gtcctgctgg cttacatcgt
    23341 ggctctggcc ggttgcctga gcgtgcgcag tattcgcgcg cacaagggtt atcgcgcgtt
    23401 gctgatattg acgggcatct tctttctcgt cctgacattc ttcgacgggc agaagctctc
    23461 gttttatctg gtgcacatcg ttccgctcta caccgcgctg ctcgccgtct ggatttactg
    23521 gtgctactcg aagcgtttcg tccctcgttg gctgctcgcg ctctgtgtct gcggcctgct
    23581 cgccatccaa ctcggcgggt tgctctatcg catgaagctc aacgtctacc agaagagtta
    23641 tctggccgcg accagtttta tcgccgcgca cgcgaaggaa gattcgatgg tgatggggag
    23701 cgcggtgctc gggttcgagt tgggttacga acgcatcgtg gacgacgtgc ggttcggttt
    23761 caggagcggc aagcggcctg acttcatcgt catcaacgac gtgtacgaag agacgatccg
    23821 gtattaccgc gcgggcggcg agggcgcgga actcgccgcg cacgtcaaca atttgctgaa
    23881 cgaggaatac gagttggttt acgaccggaa cttctatcag atttatgcgc gcaggaattt
    23941 tatgacgcgt aagtcttgag ggcgaaagaa aggtttacag catgcgagac atggcggcga
    24001 taaaagatta tgcgaaagct acgacggcgg cgcacacgcg cgcctcacag cctgtgattg
    24061 ccgtgctcat tccttgttac aacgaggagt tgacggtggc ggaagtcgtc gcagactttc
    24121 gcgcgcagtt gccggaggcg gacatctacg tcttcgacaa caactcgacg gacgctacgg
    24181 tggagcgtgc gcgcgaggct ggcgcgcagg tcttctacga gcggcggcag ggcaagggct
    24241 acgtcgtgca gtcgatgttc cggcaggtgg atgcagacat ctacatcatg gtggacggcg
    24301 acgg
//
LOCUS       DASWNS010000423        12331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_456412, whole genome shotgun sequence.
ACCESSION   DASWNS010000423 DASWNS010000000
VERSION     DASWNS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12331
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456412"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGB76_14715"
     CDS             <1..192
                     /locus_tag="VGB76_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100199.1"
                     /translation="FFSEQLEAIQDAIASKHGFQSTHHTLRMFGSCAECQQASRGTEK
                     KEESAAEPRVRFVSAPSTS"
     gene            304..879
                     /locus_tag="VGB76_14720"
     CDS             304..879
                     /locus_tag="VGB76_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0100200.1"
                     /translation="MNVEFVPEGRHGIVAEGTYLWDAAKRLGLRLPAECEGRGECDTC
                     AVIVREGATLLSGLTNAERTRLSPERLAAGERLACQTKVERGGDLVLEPVPVTEREET
                     TEEVKQDLRAEFREMPLQQKLTTLVELEAIAASQTFKAIADVPLSYFEKGLDYLVNFG
                     RTRARRERQARRPAEHVAGAKENPKSGGSAS"
     gene            965..2002
                     /locus_tag="VGB76_14725"
     CDS             965..2002
                     /locus_tag="VGB76_14725"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY0100201.1"
                     /translation="MLPHFGAARRAHTFEKLNLTLALLLLPFLYTACTNNAPTNNAQG
                     SATPAASAAPSSAPTRPAFDGERAFEHVRRMVEIGPRPAGSAELARTREYITGELEAT
                     GLKVTQDKFTSQTPVGAREFVNVTAELAGESTDVIIVSSHYDTKPYKEFRFVGANDGG
                     SSTGALIEIARSLAASGRPKPRFTYWFVFFDGEEAFCQNWSQCGKPGAPDNTYGSRRY
                     VQQLAARGETKRVRAMILLDMIGYTDLAIPRETLSTPWLVDIIWETADELGYGAQFPD
                     TEESVEDDHIPFLQAEIPVVDLIQLGGYMRYWHTPEDTLDKISPRSLQIVGETVIASL
                     PRIEQRLAGRS"
     gene            2082..3152
                     /locus_tag="VGB76_14730"
     CDS             2082..3152
                     /locus_tag="VGB76_14730"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF002898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY0100202.1"
                     /translation="MKRIAVVAGDGIGREVTDEALGVLEQVRESHGVTLEFQHFDWGA
                     DKFLREGVSLPTGALDMLTRDFDAILAGAFGDPRVKTNQHAEDILLGMRRGLDLYINL
                     RPVRLLDARLTPLRGRRIEDVDFVVFRENTEGAYIGAGGFLKKGTPHEIATQEELNTR
                     QGVERIVVAAFEYARRHGRKKVTMADKSNVQRYGGDLWQRTFREVAAGYADIEANHQY
                     VDAMAMFMVLDPAQYDVIVTNNLFGDILTDLGAALQGGLGMAASGNIHPGRVSLFEPV
                     HGSAPPLANKNVANPVGSILTAAMMLEYLGFDEASEAVEAAVGASIIAGDTTRDLGGT
                     LSTTEAGAAIRRRIGASSSANS"
     gene            3208..3981
                     /locus_tag="VGB76_14735"
     CDS             3208..3981
                     /locus_tag="VGB76_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100203.1"
                     /translation="MGSRGRDAGQKLVAIIVSLAMLALMGIPPTVIIFFATVVYFVWR
                     AVDRSEQHETRRIFDFYLAANEVLREDERRWFGFEITEVIGQGEAVVHSMADAPPLAH
                     FALGALYHRAGDHEAATEHLAYVSEAEAGDEQHCISPSPELRRYVRILRRLERDPSEG
                     PQTLAAVRNLERTRRAQLAALLEESRKLSVSSPALDSRTAARDSYAGIGHAAPREDAP
                     PRLPLAANTNAPHPLSPPPKPAPISEVLRDLYEEEKKTA"
     gene            4115..5914
                     /locus_tag="VGB76_14740"
     CDS             4115..5914
                     /locus_tag="VGB76_14740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0100204.1"
                     /translation="MATETIIGSYRIEERIGRGGMGVVYRGQHTKLPREVAIKSINPR
                     AGDHDLRHMRHRFEREAFIQSQLDHPGIVKIYDYIVAEQTYYIVMEYVEGRSLAQLIK
                     QQEGRPLVCERALGLFDQILTAVTYAHTFTYQDQDGAAHRGIIHRDLKPPNILVTPDD
                     KIKITDFGIVKVVGSETTDTFGGGYGSPRYVSPEQAEGLEVDQRSDIYSLGIILYEML
                     TGAPPFGNEREPMTRTEILRAHVELPPPLPTEINPLIPLEVESIILCALEKDPDGRYS
                     TATDFLRAIRVARERDVSDVPEEAEAQPKTYRPVGTHELHASVDNTVRQDYITQPIRS
                     GSCAVCGTEAEADEEYCLACGAELAASPATTRLAHRQAKTPEAVRQVGALRRSQRGKW
                     VVVGLIALALLSGVLIYMRFSREAERRARAAAVAPTVAPQVAAPPSMQTPPAPSSLIE
                     LAASRVAVDSSYDGYTAKPLNDGVVDVKRIAGMRYNEGNWASAETQQAHWIEFQLRQP
                     ARISAVYIYWGFDRDRFVASRRVELQTPDERGEWRTISTIEPGSNYDRAAFEFAPVET
                     ERVRILQPSQQGPRGRPFVMWVREVKVFAVAED"
     gene            complement(5989..7677)
                     /locus_tag="VGB76_14745"
     CDS             complement(5989..7677)
                     /locus_tag="VGB76_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100205.1"
                     /translation="MRIISQPEGKAKALASCALVVLVWGFFLVRAISVFAPDSVYVQP
                     FNSDSALPVLMANDERIDAFRTYIYGQDQVGAWPSLICQLIKRATGHRWTPLQIHIMQ
                     ATWLFLSFFLIVALTRSYFYLTGALFLLTLCLHPTVSHYFFVLNQRFAWQTTALFLGW
                     WSLRRSCEYQFGVISWSRGKAVIRHLLSFGFTFLAAWTSPSSVPILCVFFALECVRAR
                     ILSLRRGKASPRLAFANFAGALPLLAGFAAQRLLQANYHRFALRHFGQDYRTPVEFDW
                     GYLRINLRHQLDNLFDAPWYALTLLGVCAAPFVAFQLLSYLRRRAPEEESLSGDEGVA
                     ANPRLDLEVLLLGSCAIAVINFVTSFIFSWMRLNAYGARYLALTYMFGTFAGLLALVW
                     LVRIPKKFDAARRYAFPLLTLACFVLLALKFPPVQIEPTYRQMSEVAADLSRNAPPGT
                     PLLGGYWDTYALASLQADATLVPVPAEDQLVRTPWTPLKMREAGQVLVVHHVFSSPGG
                     AERVSAYTTFGDGQNPPAVIRQHGATLQLHTPRWYERHGYVFSLYKNQHAMGGH"
     gene            7764..10331
                     /locus_tag="VGB76_14750"
     CDS             7764..10331
                     /locus_tag="VGB76_14750"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEY0100206.1"
                     /translation="MRGLRWQSWWHARRGDVGALSIISLFFTLFFGRTILAGRFLVAA
                     DAFYYSYPLRTVAWRSLREGALPLWTPHVMAGYPLLAMSQLGLGYPLTWGYLFLSGHL
                     AEEVYILAPFLLAPAFTYAYARELGRTRLAALLAGLSFTYGGMMLSKLGVIGMPTNSA
                     MWLPLVLLAVERARTRRLIPCLLLSTFAFAMSVLNGHGQSFLYTGIVALGYGAFIYAT
                     AASARMRRVEGASLTARGQAWRPLAVAVGGLSLSLGIAAFQILETMRAVRRSVRSTLA
                     YEVFSAGAFTPLETLKSFIAPVYHYVEVSTYMPPLAFALAVYGSVCALRPEGRDARLF
                     FWVLVVVSSYVLMLGGSTPLNWLLYHVPIYNRFRYPSRHAFEWTFALSILSAYGWDAI
                     KLRRARLHGARLNDANLHDAGLQAARRMPRPGARLLALIALASSLIAGACWWRAIGQP
                     PIALAAPDTGYGTGLAEDIYIFWKLGFTLLVCVAVWQSWRVAATRWRAGLLIGAVMAS
                     CFVEPFIMASTWWFPDAKAASRFENASPAIRMLRAYAPEENRVYTRVNLYLVPGYPPT
                     PPPLELPNLPALDGIHNVAGYEQLVLERFSRALGNVGADAVNLRYDVQGAPDNSIFEE
                     RSHVLDLLNTSFVVTFANLSPAPAREGQPATLAAAPPNLSPARWETMLDREGVLVLRN
                     RRAQPRAWLVAEASAVDGEEALRRIRGESGNRSSGDDVPEFDPRRTALLEVSAEELPA
                     LPGGALAPESTIRLVRYEPTRLSLETDAPTATVAVISELFYPGWTATVDGSAQPIHLT
                     NFLLRGVAVPAGRHRIEMQYHAPAARNGALISLCTLLLIILLSVFARRARPGRVVVG"
     gene            10346..11872
                     /locus_tag="VGB76_14755"
     CDS             10346..11872
                     /locus_tag="VGB76_14755"
                     /inference="COORDINATES: protein motif:HMM:NF005545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0100207.1"
                     /translation="MTDSESLQKSVAIIGAGPAGLTAAYTLLKKSEGRIKVTVFEADE
                     QYVGGISRTVNYKGFHFDIGGHRFFSKSKEVEDLWTELLPEDMLERPRLSRIFYRGKF
                     FSYPLRALEALFKLGFIESVLCMASYVRARLFPIPDPKSFEDWVSNKFGRRLFKIFFK
                     TYTEKVWGMNCRDISADWAAQRIKDLSLISAIVNSILPPKASRPNSQQIKTLVNTFRY
                     PRKGPGMLWAACAEKVAAMGGEILMGRKVTRCEWNKADGLWTIEARDGAGTPHRIQAQ
                     HVISSAPLRQLAASLSPTLPERALGAARSLKYRDFLTVSLIVKDRGIFLDNWIYIHDP
                     GVRVGRIQNFKSWSPEMVPTPNLNCYGLEYFCFEGDGLWSSSDDALIKLATAELVQIG
                     LAEAGDVLDGCVVRQAKAYPVYDDAYAEHVALLRSELEEHFPTLHLVGRNGMHKYNNQ
                     DHAMMTAMLCAENILADARVYDVWCVNQDAEYHEQGGRDTGSSVSGMRLVPSRLSADK
                     "
     gene            11954..>12331
                     /locus_tag="VGB76_14760"
     CDS             11954..>12331
                     /locus_tag="VGB76_14760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEY0100208.1"
                     /translation="MLTAEIIAIGSELLTPDRADTNSLWLTEKLNAIGIEVKLKTIVG
                     DDDARLEETIKDALRRSRVVITTGGLGPTEDDITRKIAARAMGRRLVLNEAVLEGIRE
                     KFMRWGRKMPEINTRQAMIIEGAE"
BASE COUNT         2419 a         3789 c         3878 g         2245 t
ORIGIN      
        1 ttcttctccg aacaactcga agctattcag gacgcgattg cttccaagca cggcttccaa
       61 tccacgcatc acaccctgcg gatgttcggc tcatgtgccg agtgccagca ggccagccgg
      121 ggcacagaga agaaagaaga gtcagccgcc gaaccgcgcg tgagatttgt gagcgcgccg
      181 agcacgagct agccagtcac ctttttacat ttgcccttcg ttagtgtaat ttccagatcg
      241 tctcccgtga aaaaaataac ctgatgattt gagaacctgt ggtgggggtg gtgtgcaatg
      301 agtgtgaacg ttgaattcgt gccggaggga cggcacggca tagtggcgga agggacatac
      361 ttgtgggacg ccgccaagcg tctcggtctg cgtctgcctg cggagtgcga ggggcgcggc
      421 gagtgcgaca cgtgcgccgt aatcgtcaga gaaggcgcga cgctgttgtc cggtctgacg
      481 aatgcggagc gcacgcggct cagccccgaa cggctcgcgg cgggcgaacg cctcgcctgc
      541 cagacgaagg ttgagcgcgg cggcgacctc gtcctcgaac ctgtccccgt cacggagcgc
      601 gaggagacga cggaggaagt gaagcaagac ctccgcgcgg agtttcgtga gatgcctttg
      661 cagcaaaaac tgaccacgct cgtcgaactg gaagccatcg ccgcctcgca aaccttcaag
      721 gccatcgccg acgtgcccct ctcatatttt gaaaaggggc tggattacct cgtcaacttc
      781 ggacgcacgc gtgcgcgacg ggaacgacag gcgcgtcgcc ccgcggagca cgtcgcgggc
      841 gcgaaggaga atccgaagtc cggcgggtct gcttcctgaa ctcttttccg aaggctggcg
      901 tgcgcgccgc cacacgacgc gccttccgtc gcgccgcgct ctttgcgcct catccatgaa
      961 gaacatgctc ccacacttcg gcgcggcgcg acgcgcacac actttcgaga agttgaattt
     1021 aacgctcgct ctcctcctgc tcccgtttct ctacaccgcc tgtacgaata atgcgccgac
     1081 gaacaacgcg cagggcagcg cgactcccgc cgcctccgcc gctccttcgt ccgcgccgac
     1141 gcgccccgct ttcgacggcg agcgtgcttt cgagcatgtg cgcaggatgg tcgagatcgg
     1201 gccgcgcccc gcaggctccg ccgaactcgc gcgcacgcgc gaatacatca cgggcgaact
     1261 cgaagccacc gggttgaaag tgacgcaaga taagttcacg tcgcaaacgc ccgtcggcgc
     1321 acgcgagttt gtgaacgtca cggcggaact cgcgggcgaa tcgacggacg tgatcatcgt
     1381 ctccagccat tacgacacga agccttacaa ggagtttcgc ttcgtcggcg caaacgacgg
     1441 cggctcttcc acgggcgcgc tcatagagat cgcacgctcg ctcgccgcga gcggacgccc
     1501 gaagccgcgc ttcacttatt ggttcgtctt cttcgacggc gaagaggctt tctgccagaa
     1561 ctggagccag tgcggtaagc ccggcgcgcc cgacaacact tacggcagcc gtcgctacgt
     1621 gcagcaactc gccgcgcgcg gtgagacgaa acgcgtccgc gccatgatcc ttctggacat
     1681 gatcggttac accgatctgg cgatcccgcg cgaaacgctg agcacgcctt ggctcgtaga
     1741 catcatctgg gagacggcgg acgaactcgg ctacggcgcg cagtttcccg acacggaaga
     1801 gagcgtggaa gacgaccaca tcccgtttct gcaagccgag attccggtcg tcgatctgat
     1861 ccaactcggc ggctatatgc gttactggca cacgcccgaa gacacgctcg ataaaatctc
     1921 accgcgcagt ttgcagatcg tcggcgagac ggtcatcgcc agcttgccgc gcatcgaaca
     1981 acgcctcgcc ggacgatctt aaacgggcat gtagagacgg cggaagtaat gatagaagag
     2041 acgcggaagc gacggcagag aagatgatga cgacaaggaa cgtgaagaga atcgcggtcg
     2101 tcgcaggcga cggcatcggg cgagaggtga cggacgaagc tctcggcgtg ctcgaacagg
     2161 tgcgcgagtc gcacggcgtc acgcttgagt tccagcattt cgactggggc gcggataagt
     2221 ttttgcgcga gggcgtgagc ctccccaccg gcgcgctcga catgctgacg cgcgatttcg
     2281 acgcgatcct cgcgggcgcg ttcggcgatc cccgcgtcaa gacgaaccag cacgcggaag
     2341 atattctgct gggcatgcgg cgcggcctcg acctgtacat caacctgcgc cccgtgcgcc
     2401 tgctcgacgc gcgcctgacg ccgcttcgcg gacgccgcat tgaagacgtt gacttcgtag
     2461 tctttcgcga gaacacggag ggcgcgtaca tcggcgcggg cgggttcttg aagaagggga
     2521 cgccgcacga gatcgccacg caggaggaac taaacacgag acagggcgtg gagcgcatcg
     2581 tcgtcgcggc cttcgagtac gcgcgcaggc acggcagaaa aaaagtgacg atggccgaca
     2641 agtcgaacgt gcagcgatac ggcggcgacc tgtggcagcg cacctttcgc gaagtggcgg
     2701 cgggctatgc cgacatcgaa gcgaatcatc aatacgttga cgcgatggcg atgttcatgg
     2761 tgctcgatcc ggcgcagtat gatgtgatcg tgacgaacaa tctgttcggc gacatcctga
     2821 ccgatctggg cgcggcgttg cagggcggat tgggaatggc cgcgtcgggc aacattcatc
     2881 cggggcgcgt ctctctgttt gagccggtgc acggcagcgc gccgccgctg gcaaacaaga
     2941 atgtggcgaa tccggtaggg tctattctga ccgcagcgat gatgttagaa tacctgggat
     3001 tcgacgaagc gagcgaagcg gtcgaagccg ccgtcggcgc aagcattatc gcaggcgaca
     3061 cgacgcgcga tctcggcgga acgctctcca cgacggaagc cggagccgcg attcgtcgtc
     3121 gcatcggggc gagttcgtcg gcgaactctt aaaatgagca tggcacatgg gggttagcgg
     3181 gtcgcaaggc ggcgcgcgag aagtcttatg ggtagtaggg gcagagacgc aggacagaag
     3241 ttagtcgcga tcatcgtgag cctcgcgatg ctcgcgttaa tgggcatccc gccgaccgtc
     3301 atcatctttt tcgccaccgt cgtctatttc gtctggcgcg cggtcgatcg ctccgagcaa
     3361 cacgagacgc gccgcatctt cgatttctac ctcgccgcca acgaggtcct gcgcgaagac
     3421 gaacgccgct ggttcggctt tgagatcacg gaagtgatcg ggcagggcga ggccgtcgtt
     3481 cattcgatgg ccgacgcgcc gccgctggca cacttcgcgc tcggcgcgct ctatcatcgc
     3541 gcgggcgacc acgaagcggc gaccgaacac ctcgcttacg tctccgaagc ggaagcgggc
     3601 gacgaacagc attgcatttc gccttcgccc gaactgcgcc gttacgtgcg catactgcga
     3661 cggctcgaac gcgacccttc ggagggtccg cagacgctcg ccgccgtgcg caatctcgaa
     3721 cgcacgcgtc gcgctcaact cgccgcgctg ctcgaagaga gtcgcaaact cagcgtctca
     3781 tctccggcgc tcgatagcag gacggcggcg cgcgactcat acgcgggcat agggcatgcc
     3841 gccccgcgcg aagatgctcc gccccgtctg cccctcgccg caaacaccaa cgcgccgcac
     3901 ccgctctcgc ctccgcccaa gcccgcgccg atctccgaag tcctgcgcga cttgtatgag
     3961 gaagagaaga agaccgcttg aaaaaacgtc agttgtccgt ggtcagttgc ccgttgtttg
     4021 tgctgaccgg agaaatggct aaagattgaa atggacaaac tgcccgaagc acaaaaataa
     4081 ccgacacccg accacggaca actgacggct tttaatggcg actgagacga tcatcggcag
     4141 ttatcggatc gaggagcgca tcgggcgcgg ggggatgggc gtcgtctatc gcggccagca
     4201 tacgaagctc ccgcgcgaag tcgccatcaa atccatcaac ccgcgcgcag gcgatcacga
     4261 cctgcgccac atgcgccatc gcttcgagcg cgaagccttc atccagtcgc aactcgatca
     4321 cccgggcatc gtcaaaatct acgactacat cgtcgccgag cagacctact acatcgtgat
     4381 ggagtatgtc gaagggcgct cgctggcgca gttgatcaag cagcaggaag gccgcccgct
     4441 cgtttgcgaa cgcgcgctcg gcctcttcga tcaaattctc accgccgtca cctacgcgca
     4501 caccttcacc tatcaggatc aggacggcgc ggcgcatcgc ggcatcattc accgcgactt
     4561 gaagccgccg aacatcctcg tcacgcccga cgataagatc aagatcacgg acttcggcat
     4621 cgtcaaagtc gtcggctcgg agacgaccga cacgttcggt gggggctacg gctcgccgcg
     4681 ctacgtctcg cccgaacagg cggaaggatt ggaagttgac caacgttcgg acatctacag
     4741 tctggggatc atcctctacg agatgctgac gggcgcgccg cccttcggca acgagcggga
     4801 accgatgacg cgcacggaga ttttgcgcgc acacgtcgaa ctgccgccgc cgctgcccac
     4861 cgagatcaat cccttgatcc cgctcgaagt cgagtccatc atcttgtgcg cgctcgaaaa
     4921 agacccggac ggtcgctact cgacggcgac agattttcta cgcgcgatca gggtcgcgcg
     4981 agaacgcgat gtgagcgatg ttcccgaaga ggccgaggcg cagccgaaaa cttatcgccc
     5041 cgtcggcacg cacgagttgc acgcgagcgt ggacaacacc gtgcggcagg attacatcac
     5101 gcaaccgatc cgcagcggtt cgtgcgccgt gtgcggcacg gaggcggagg cggacgaaga
     5161 gtattgtctg gcgtgcggcg cggaactcgc ggcgtcgccc gccaccacca gactcgcaca
     5221 ccggcaggcg aagacgcccg aggccgtcag gcaagtcggc gcgctgcgaa ggtcgcaacg
     5281 cggcaagtgg gtggtcgtcg gactcatcgc gctcgcgctc ttgagcggcg tgctcatcta
     5341 catgcgcttc tcgcgcgaag ccgagcggcg cgcgcgtgcg gctgccgtcg cgccaaccgt
     5401 cgcgccgcaa gttgccgcgc cgccttcaat gcagacgccg cccgcgcctt cgtccctcat
     5461 cgaactcgcc gcgtcgcgcg tcgcggttga ttcaagttat gacgggtaca cggcgaagcc
     5521 tctgaacgac ggcgtggttg atgtgaagcg gatcgcgggg atgcgttaca acgaagggaa
     5581 ctgggcttcg gctgagacgc agcaagccca ctggatcgag tttcaactac gacaacccgc
     5641 gcgcatctcc gccgtctaca tttactgggg cttcgaccgc gaccgcttcg tcgcctcgcg
     5701 ccgcgtggaa ctacagacgc ccgacgagcg cggcgaatgg cgcacgatct cgactatcga
     5761 acccggcagc aattacgacc gcgcggcctt cgagtttgcg cccgtcgaga cggagcgcgt
     5821 gcgcatcctt caaccgtcgc agcagggacc gaggggtcgg cctttcgtga tgtgggtgcg
     5881 tgaggttaaa gtcttcgctg tggccgaaga ctaaatcgat agactaattc aatccgctcg
     5941 ccacgctcga tctaaaaatc tctaaatcgc atcccgccgc gccgcccgtc aatgcccgcc
     6001 cattgcgtgc tgattcttgt agagtgagaa gacgtaaccg tgcctttcat accagcgcgg
     6061 cgtgtgcagt tgaagggtcg cgccgtgctg gcggataact gcgggcgggt tctgcccgtc
     6121 gccaaacgtc gtgtacgcgc tcacgcgttc agccccgccg ggagaagaga agacgtgatg
     6181 cacgacaagc acctgccccg cctctcgcat cttgagcggt gtccacggag tcctgacgag
     6241 ttgatcttct gcgggaacgg gaacgagcgt cgcgtctgct tgcagcgaag ccagcgcgta
     6301 tgtgtcccaa taaccgccga gcaggggcgt gccgggcggc gcattccgtg ataagtctgc
     6361 cgccacctcg gacatttgcc tgtaggtcgg ctcaatttga accggaggaa attttaaggc
     6421 gagcaggacg aagcaggcga gggtgagcag cgggaaggcg tagcgacgcg cggcgtcgaa
     6481 ttttttcggg atgcggacga gccacaccag cgcgagcagt ccggcaaacg tcccgaacat
     6541 gtatgtcagg gcaagatagc gcgccccgta agcgttgagg cgcatccacg aaaatataaa
     6601 cgaggtgacg aagttgatga cggcaatggc acaactgcca agcagcagca cttcgagatc
     6661 gagtcgcgga ttcgccgcga cgccttcgtc gccgcttaga ctctcttctt ccggcgcgcg
     6721 gcgtcggaga taactgagga gttgaaacgc gacgaacgga gcggcgcaca cgccgagcag
     6781 cgtgagagcg taccacggcg cgtcgaagag attgtccaac tggtggcgga gattgatccg
     6841 caggtagccc caatcgaatt cgaccggcgt gcgataatcc tgcccaaagt gtctgagcgc
     6901 gaaacgatgg tagttagctt gcagcagacg ttgggcggcg aagcccgcga ggagcggcag
     6961 cgcgcccgcg aaattggcga acgcgagacg cggcgaagcc ttcccgcgac gaagcgacag
     7021 gatgcgcgca cgcacacatt ccagcgcgaa gaagacgcag aggatcggga cgctcgaagg
     7081 cgaagtccac gcggcgagga aggtgaaacc aaaagagagc aggtgccgga tgacggcttt
     7141 gccgcgactc cacgaaatca ccccgaattg atattcgcag cttcggcgca gactccacca
     7201 accgagaaac agcgcggtcg tctgccaggc gaagcgttgg ttgagcacga agaagtagtg
     7261 gctgacggtc gggtgcaggc agagcgtcaa cagaaacagc gcgcccgtta gatagaagta
     7321 tgatctcgtc agggcgacga ttaagaagaa gctgaggaag agccacgtcg cctgcatgat
     7381 gtggatttgc agtggcgtcc agcggtggcc ggtggcgcgc tttatcagct ggcaaatgag
     7441 agacggccac gcgccgacct gatcctgacc gtagatgtac gtgcggaagg cgtcgatccg
     7501 ttcgtcgttc gccatcagca cggggagcgc gctatcggag ttgaagggct gcacgtagac
     7561 gctgtcgggc gcgaagacgg agatcgcgcg gacgaggaag aaaccccaca ccagcacgac
     7621 aagggcgcag gacgccaatg ctttggcttt tccctccggt tgcgagataa tccgcatttc
     7681 caccccaccc agacaaagat gaccaagagt aagggaagcg catcaataat caaagccatg
     7741 ttgcagttga tgacggtcgc gtgatgagag ggttgcgctg gcagagttgg tggcacgcgc
     7801 ggcgcggcga cgtgggcgcg ctctcgatca tctctctctt cttcactttg ttcttcgggc
     7861 gcacgatcct tgccgggcga ttcctcgtcg ccgccgacgc cttctattat tcatacccgc
     7921 tgcgcacggt ggcgtggcgg tcgctccgcg aaggcgcgtt gccgttgtgg acgccgcacg
     7981 tcatggccgg ttatccgttg ctggcgatgt cgcagttggg gctgggctac cctttgacgt
     8041 ggggctatct tttcctgtcc ggccatctcg ccgaagaggt ctatatcctt gcgccattcc
     8101 tgctcgcccc cgccttcacc tacgcctacg cgcgcgaact ggggcgcact cgcctcgccg
     8161 cgctcctcgc cggactctct ttcacttacg gcggcatgat gttgagcaaa ctcggagtga
     8221 tcggcatgcc gacgaactct gcgatgtggt tgccgctcgt cctgctcgcc gtcgaacgcg
     8281 cacgcacgcg ccgcctgatt ccctgcctgt tgctctcgac gtttgccttt gcgatgtcgg
     8341 ttctcaacgg gcacgggcag agctttttgt acaccggcat cgtcgcgctc ggttacggag
     8401 cgttcatcta cgcgaccgct gccagcgcaa ggatgcgacg cgtggaaggc gcaagcctca
     8461 cggcgcgcgg gcaggcgtgg cgaccgctgg cggtagcagt tggcgggctg tcgttatcgc
     8521 tcggcatcgc cgcttttcag attcttgaga cgatgcgcgc cgtgcgccgc agcgtccgca
     8581 gcacgctcgc ttacgaagtg ttcagcgcgg gagcgttcac gccgctcgaa actctgaaat
     8641 cgtttatcgc gcccgtctat cattacgtcg aagtatcaac ttacatgccg ccgctcgcgt
     8701 tcgcgctggc cgtgtatggg agcgtctgcg ccctgcgtcc ggaggggcga gacgcgcgtc
     8761 tgtttttctg ggtgctcgtg gtcgtgtcga gctacgtgtt gatgctcggc gggagcacgc
     8821 cgctcaactg gctgctctac catgtgccga tctataaccg cttccgctat ccctcgcgcc
     8881 acgccttcga gtggacgttc gcgctcagca ttctgtcggc ttacgggtgg gacgcgatca
     8941 aactgaggcg agcgcgcctg cacggcgcac gcttgaatga tgccaacctg cacgatgcgg
     9001 gtttgcaggc tgcgcgccgg atgccgcgac cgggcgcgcg gctcctggcc ttgatcgcgc
     9061 tggcgtcgag tttgatcgcg ggcgcgtgct ggtggcgggc aatcggtcaa cccccaatcg
     9121 cgctggctgc accggatacg gggtacggga cggggctggc cgaagacatc tatattttct
     9181 ggaaactcgg ctttacgctg ctcgtctgcg tcgccgtgtg gcagagttgg cgtgtggcgg
     9241 cgacgcgctg gcgcgcggga ctgttgatcg gcgcagtcat ggcaagctgt ttcgtagagc
     9301 ctttcatcat ggcctcgacg tggtggtttc cggacgctaa agccgcgagc cgtttcgaga
     9361 acgcgtcgcc cgccatacgc atgctgcgag cctacgcgcc ggaagagaat cgcgtctata
     9421 cgcgcgtcaa tctctatctc gtgccgggtt atccgccaac gccgccgccg ctcgaactcc
     9481 ccaacctgcc cgcgctcgac ggcatccaca acgtcgccgg ttatgaacaa ctcgtgctcg
     9541 aacgtttcag ccgtgcgctc ggcaacgtcg gcgcggatgc ggtcaaccta cgctacgacg
     9601 tgcagggtgc gcccgacaac agcatctttg aagagcgttc gcacgtcctc gatctgctca
     9661 acacttcgtt cgtcgtcacc ttcgccaacc tctcgcccgc acccgcacga gaaggacagc
     9721 ccgcgacgct tgccgccgcg ccgccgaatt tgtctccggc gcgttgggag actatgctcg
     9781 accgcgaagg cgtgctggtg ttgcgcaatc gcagggcgca gccgcgcgcg tggctggtgg
     9841 cggaagcgtc ggcggttgac ggcgaggaag cgttgcgccg catacgtggc gagagcggca
     9901 accgtagcag cggcgacgac gtgccggagt tcgacccgcg ccgaaccgcc ctgctcgaag
     9961 tgagcgcgga agagttgcca gcccttcccg gcggcgcgct cgcgcctgag agcacgattc
    10021 gtctcgttcg atacgaaccg actcgcctgt cgctggaaac agatgcgccg acggcgaccg
    10081 tcgcggttat cagcgaactt ttctatccgg gatggacggc gacggtggac ggctccgcgc
    10141 agccgattca tctgacaaac tttctgctgc gcggcgtggc ggttccggca gggcggcatc
    10201 gtatcgagat gcaataccat gcgcccgccg cgcgtaacgg cgcgttgatt tctctctgca
    10261 cgttgttatt gattatcctg ctgtcggtct tcgcgcggcg tgcgcgtccg gggcgcgttg
    10321 tagtgggttg aggagattat taattttgac cgacagtgag agcttgcaaa aatcggtggc
    10381 gatcatcggg gccggtcccg caggtctgac ggcggcttat actctgttga agaagtccga
    10441 aggacgaatc aaagtcactg tcttcgaggc cgatgagcaa tacgtcgggg gaatttcgcg
    10501 caccgtcaac tacaagggat ttcatttcga catcggcggg catcgcttct tctccaagtc
    10561 gaaagaggtg gaagacctct ggacggaact cctgcccgaa gacatgctgg agcgtccgcg
    10621 cctctcgcgc atcttctatc gcggcaagtt cttctcctac cctctgcgcg cgctcgaagc
    10681 tctgttcaaa ctcggcttca tcgaatccgt cctctgcatg gcttcatacg tgcgcgcacg
    10741 cctgttcccc attcccgacc cgaagagttt tgaagattgg gtcagcaata aatttggccg
    10801 cagactgttc aagattttct tcaagacgta cacggagaaa gtttggggca tgaactgtcg
    10861 ggacatctcc gccgattggg ctgcgcagcg catcaaggat ttgtcgctca tctcggccat
    10921 cgtcaacagt atcctgccgc cgaaagcctc gcgcccgaac agccagcaga tcaaaacgct
    10981 cgtcaacact tttcgctacc cgcgcaaagg tcccggcatg ttgtgggcgg cctgcgcgga
    11041 gaaagtcgct gcgatgggcg gcgagattct catggggcgg aaggtgacgc gctgcgaatg
    11101 gaacaaggcc gacggcctct ggaccattga agcgcgcgac ggcgcgggca cgccgcacag
    11161 gattcaggcg cagcacgtca tctcgtccgc gccgctgcgc caattggccg cgtcgctttc
    11221 gccgacgttg cccgaacgcg cgctcggcgc ggcgcgctcg ctcaagtata gagactttct
    11281 gaccgtctct ctgatcgtga aagaccgggg catctttctg gacaactgga tttacataca
    11341 cgatccgggc gtgcgcgtgg gcaggattca gaacttcaaa tcgtggtcgc cggagatggt
    11401 tccgacgccc aacctcaatt gctacggcct ggagtatttc tgcttcgaag gcgacgggct
    11461 gtggtcttcg tcagacgacg cgctgatcaa actcgccacc gcagagcttg tccaaatcgg
    11521 gctagcggag gctggcgatg ttctggacgg ctgcgtcgtc aggcaggcga aagcgtatcc
    11581 ggtctatgac gacgcttacg ccgaacacgt cgcgctgctg cgctccgagt tggaggagca
    11641 tttccccacg ctccacctcg tcgggagaaa cgggatgcat aaatataaca atcaagacca
    11701 cgcgatgatg acggccatgc tctgcgccga gaacattctc gcggacgcgc gcgtctacga
    11761 cgtgtggtgc gtcaatcagg acgccgaata tcacgaacag ggaggcaggg acacagggag
    11821 ttctgtgagc gggatgcggc tcgtcccttc acgcctcagc gcggacaagt agccgtgcga
    11881 gcgttcgtgc gtcaccttga aatttgtatg atccgtattg acagcgccgc ctttctatct
    11941 aatcattgaa acaatgctca ccgctgaaat catcgccatc ggctcggagt tgctgacgcc
    12001 ggatcgtgcg gacacgaaca gtctctggtt gacggagaaa ctgaacgcca tcggcatcga
    12061 agttaaactc aaaactatcg tcggcgacga cgacgcgcgc ctcgaagaga cgatcaagga
    12121 tgcgctgcgc cgctcacgcg tcgtcatcac gacgggtggt ttggggccga ccgaagacga
    12181 catcacgcgc aagatcgcgg cgcgtgcgat ggggcgtcgc ctcgtgttga atgaagcggt
    12241 gctcgaaggc atccgcgaga agtttatgcg ttggggacgc aagatgcctg agatcaacac
    12301 gcggcaggcc atgatcatcg aaggcgcgga g
//
LOCUS       DASWNS010000424         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_405358, whole genome shotgun sequence.
ACCESSION   DASWNS010000424 DASWNS010000000
VERSION     DASWNS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405358"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VGB76_14765"
     CDS             complement(<1..241)
                     /locus_tag="VGB76_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100209.1"
                     /translation="MKRRFNNIASRSRLLTRLLPLLLLLCAVGSTASAQTYDLGGGGL
                     PTITGALNGSVSGTSSTTQDLVVTVNFGEVSPLNAN"
     gene            complement(382..1104)
                     /locus_tag="VGB76_14770"
     CDS             complement(382..1104)
                     /locus_tag="VGB76_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100210.1"
                     /translation="MFNSLKQLSQVTFFAVLLVLTATVAQAQVNTGGTVTISGTVSKF
                     VELNSGGAVTLAGNSGGGISTDGVADNALNVVVNLGELGPSNTSSFVTATVPLKMRSN
                     TNYILSMSASIVSSGSSTSKIGAADIGFGLGTVNRPNSGPSSIGVAAGADVDNTSGDP
                     TNVANGAINAASGRFEYTAARSHLGAFSASTTVLSGPRILNAVPRTNGNGLTVPAIFS
                     VKPQFYENGTMTATASFTIAAP"
BASE COUNT          302 a          410 c          504 g          310 t
ORIGIN      
        1 tgttcgcgtt gagcggggag acttcgccga agttgaccgt gacgacgagg tcttgcgttg
       61 tgctcgaagt gccgctgacg gagccgttga gcgcgcccgt gatggttggc aggccgccgc
      121 cgccgaggtc gtacgtctgc gcggaggccg tcgaaccaac cgcgcacaac aacagcagca
      181 gcggcaggag ccgcgtgagc aggcgggaac ggctggcaat gttgttgaag cgtcgcttca
      241 taatctttcg ggcggcaagg ccggaggtgc gcgtccgtct gcgtctatag agttcaagcg
      301 ccgtcacctg cgagagaggt gacgacgcct gaaacgaccg gctgatgcac ggcgcgcgag
      361 tttgaatgct gcctccaact attagggagc agcgatggtg aagctggccg tggccgtcat
      421 cgttccgttc tcatagaact gcggtttgac ggaaaagatc gccggaacgg tcagaccgtt
      481 gccgttggtg cggggcactg cgttcaggat acgcgggccg ctgagaaccg tggtcgaagc
      541 ggaaaacgcg ccgaggtgcg aacgggctgc cgtgtattca aacctgccgc tcgccgcatt
      601 gatcgcgccg ttggcgacgt tggtcgggtc gcccgaagta ttgtctacgt ctgcgcccgc
      661 cgccacgcct atcgaactcg ggccgctgtt gggacgattg actgtgccca gaccgaagcc
      721 gatgtccgcc gcgccgatct tgctggtgga cgaaccggaa ctcacgatgg aggcgctcat
      781 cgagaggata tagttagtat tgctgcgcat cttgagcggc acggtggccg tcacgaagct
      841 gctggtattg gaagggccga gttcgccgag gttgaccacc acgttgagcg cgttatcagc
      901 gacgccgtca gtggagatgc cgccgccgga gttgcccgcg agcgtcaccg cgccgccgga
      961 attgagttcg acgaatttcg aaaccgtgcc gctgatcgtc accgtgccgc cggtgttgac
     1021 ctgcgcctga gcaaccgttg cagtcaggac gagcaggaca gcaaaaaaag ttacctgtga
     1081 gagctgtttg agtgaattga acataacttc tagcatttct ccttctaaag ggggatcacg
     1141 atcaggcatt tcaggggtgt ccgctcgtgg gtgtctaagg taggcggtag ttcgggttcc
     1201 cgtaaggaag tcccaccgcg atcttgactt acaatccgcg tgccatgcgg cggaggagag
     1261 cgcgggcgag gggcaattga tgaaacgata cggtttgacg ctttgggcga aacgcccgtg
     1321 acattaattg cgatgcaggc gtgacagtaa ccgctgactt tttgtcagag cttatgactt
     1381 tgtgtcagcc ttcatacgca ataacgtcga gttctttccc gcttctgtcg ctcaggctcc
     1441 ggcggtctga aagatgggac ttaaaaacct tcgccaacgc aggcgcagag aaagggggcg
     1501 cagcgaaaga gttgaatctt tcgctg
//
LOCUS       DASWNS010000425         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_326344, whole genome shotgun sequence.
ACCESSION   DASWNS010000425 DASWNS010000000
VERSION     DASWNS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326344"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VGB76_14775"
     CDS             complement(<1..1551)
                     /locus_tag="VGB76_14775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY0100211.1"
                     /translation="MSKVIELIPLGGVGEFGMNCMAVRYGDEMIVVDAGMGFPEESAY
                     GVDVCVPDFDFLDEYREHITAIVLTHGHEDHLGALPYILKKFNVPVYASHYTVGLAES
                     KLEEHNLLGDVLIHRVEPREVVEIGVFKVEFIRASHSLIDCFSLAITTPVGTVIHTGD
                     YKVDETPVIGEPIDLRTLRRYGQEGVLALLSDSTNATVPGRTPSERAVIPAFEEIFAE
                     AEGRIIVAAFASSIHRLQIVFDVAQQFDRKVCVLGRSMLKNVEIAERLGYLDVHENEM
                     VSFNQAKQMPDDEIVYLITGSQGEPRAVLSQLASQSYKGLSIEAGDTVVLSARIIPGN
                     ERAISRLIGDIYKRGGNIIDEKRRLIHVSGHASQEDIRIMTEAVRPKFVVPIHGEYRM
                     LYRHKEFVKNHLGYADENVIMIENGDVLELTADRATVIEKRDVGRTFIDDSGFEEIGR
                     ETVRERKQMAYDGVLTPVLTLNEETGELEFPPEIMARGVVGADGSNGFIKNLQRIITE
                     AVAKAPATE"
     gene            complement(1702..2520)
                     /gene="rsmA"
                     /locus_tag="VGB76_14780"
     CDS             complement(1702..2520)
                     /gene="rsmA"
                     /locus_tag="VGB76_14780"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY0100212.1"
                     /translation="MGAKKSLGQNFLVDEGVAARIVREVAPRADETVIEIGAGRGALT
                     ARLAGQVGRLVAIEFDRELVPVLEKRFGGRENFTLIEGDALAVDYCAAIAPASQARVV
                     ANLPYNISTAILQRLFEQRRCLTEMVLMLQREVVERITAAPGTGERGYLSVLTEAYCE
                     AHPLFDVAPEAFRPVPKVWSTIVRLRVRASVAASVPDEALLWRIVSAGFAQRRKTIFN
                     NLRHAPPPLLPQVEAAGGASALLDAAGIEAHRRAETLTLAEWVSLARTLESHTR"
BASE COUNT          492 a          800 c          770 g          523 t
ORIGIN      
        1 ctccgtcgca ggagctttcg ccacggcctc cgtgatgatg cgctgcaagt tcttgatgaa
       61 gccgttcgag ccgtctgcgc cgaccacgcc gcgcgccatg atctccggcg ggaattcgag
      121 ttcgcccgtc tcttcgttca gagtcagcac gggagtgagt acgccgtcgt aagccatctg
      181 cttgcgctcg cgcacggttt cgcgcccgat ctcttcaaag ccggaatcgt ctatgaacgt
      241 gcgcccgaca tcgcgtttct cgatgacggt ggcgcggtcg gcggtgagtt cgagcacgtc
      301 gccgttctcg atcatgatga cgttttcatc ggcatagccc agatggtttt tgacgaactc
      361 tttgtgccga tagagcatgc gatactcgcc gtgaatcggc acgacaaact tcggcctgac
      421 ggcctccgtc ataatccgta tgtcttcctg cgaggcatgg ccgctgacgt ggatgagccg
      481 acgcttctcg tcaatgatgt tgccgccgcg cttgtagatg tcgccgatga gccgcgagat
      541 ggcgcgctcg ttgccgggga tgatgcgcgc cgagagcacc accgtgtcgc ccgcttcgat
      601 gctcagccct ttgtaggatt gcgaagcgag ttgcgagagc actgcgcgcg gttcgccttg
      661 cgagcctgtg atgagataga cgatctcgtc gtcgggcatc tgcttcgcct gattgaacga
      721 aaccatctcg ttttcgtgca cgtcgagata gccgagccgc tccgcgatct ccacgttctt
      781 caacatcgaa cgaccgagca cgcagacctt ccggtcgaac tgctgcgcca cgtcgaagac
      841 gatctgcaaa cgatgtatgg aagaggcgaa ggcggccacg atgatgcgcc cctccgcttc
      901 cgcgaaaatc tcttcgaagg cgggaatcac ggcgcgttcc gaaggcgtcc gaccgggcac
      961 ggtagcgttg gtcgaatctg acaggagcgc gagcacgccc tcctgcccgt aacgccggag
     1021 cgtccgcagg tcaatcggtt cgccgatgac gggcgtttcg tcaaccttgt agtcgccggt
     1081 gtggatgacg gtgcctacgg gcgtcgtgat ggcgagcgag aagcaatcga tcagggaatg
     1141 cgaggcgcgt atgaactcga ctttgaagac gccgatttcg accacttcgc gcggctcgac
     1201 gcgatgaatc agcacgtcgc ccagcaggtt gtgctcttcc agtttgctct cggccagccc
     1261 caccgtgtag tgcgaagcgt agaccggaac gttaaatttt ttgaggatgt agggcagcgc
     1321 gccgaggtgg tcttcgtgcc cgtgcgtgag gacgatggcc gtgatatgtt cgcggtactc
     1381 gtccagaaaa tcgaaatcgg gcacgcacac gtcaacgccg taggcgctct cttcggggaa
     1441 gcccattccg gcatcgacga cgatcatctc gtcgccgtag cgcaccgcca tgcagttcat
     1501 cccgaactcg ccgactccgc cgagtgggat cagttcaatc actttgctca ctattatttc
     1561 ttactctcca gactaaccga tgaacattca tcgcaggata taatcttcgg gggaaggtta
     1621 actcgacagt ataacacaag gcgtcgcatt tcccttgagc cggatgagga ttcgcctgtt
     1681 gacagggctt ttgagtgggg ttcaacgcgt gtgcgattcg agcgtgcgcg cgagcgacac
     1741 ccattcggcg agcgtcaaag tctccgcgcg cctgtgcgct tcgatgcccg ccgcgtcgag
     1801 cagtgcgctt gcgccgcccg ccgcttccac ctgtggcaat aagggtgggg gcgcgtggcg
     1861 caggttgttg aagatggtct tgcgccgctg cgcgaagccc gcgctcacga tgcgccataa
     1921 caacgcttca tccggcaccg acgccgccac gctcgcgcgg acgcggaggc gcacgatggt
     1981 gctccacacc ttcggcacag gacgaaacgc ttccggcgca acgtcgaata ggggatgcgc
     2041 ctcgcagtag gcttcggtca acacggagag atagccgcgc tcgcccgtgc ctggcgcggc
     2101 agtgatgcgt tccaccactt cgcgttgcag catcaagacc atctccgtca ggcaacgccg
     2161 ctgctcaaag agtcgttgca ggatggccgt cgaaatgttg taaggaagat tggcgacgac
     2221 gcgcgcctga gacgcaggcg cgatggccgc gcaatagtca accgcgagcg catcgccctc
     2281 gatgagcgtg aaattctcgc gcccgccgaa cctcttttcc aacacgggca caagttcgcg
     2341 gtcgaactcg atggcgacca gacgcccgac ctgccccgcg agccgcgccg tcagagcacc
     2401 acgccctgca ccaatctcga tcacagtctc atccgcacgc ggcgcaacct cacgcacgat
     2461 gcgcgccgcc acgccttcgt ccacgagaaa attctgaccg agactttttt tcgcgcccac
     2521 agtttaaaaa cagtgacgag tgacaattga cgggtgacga ttcacgattg acgttctttt
     2581 ctcgt
//
LOCUS       DASWNS010000426         8474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_432150, whole genome shotgun sequence.
ACCESSION   DASWNS010000426 DASWNS010000000
VERSION     DASWNS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8474
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432150"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VGB76_14785"
     CDS             complement(<1..336)
                     /locus_tag="VGB76_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100213.1"
                     /translation="MENVKHRMEGLRVWLRRVMPRAAILSAALFALWLMIHNSRFYRE
                     GIVGKLFTLALVLIYFTAAYYGFQGLRWLKSRLMWRVRRRLVITYLFVGLTPIVLLIT
                     LAAMVDFFGL"
     gene            complement(469..1413)
                     /locus_tag="VGB76_14790"
     CDS             complement(469..1413)
                     /locus_tag="VGB76_14790"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY0100214.1"
                     /translation="MKTAIIHHPVYEEHDTGDNHPESPARYVAVMEALRGDDGLWSQL
                     LELEAPLAPRGDVQAAHTTQHFKRVERAVSEGRGYLDADTIISMRSLDAALRAAGGAC
                     RAVDAVMAGEARNAFVPARPPGHHATSENAMGFCLFNAVAVAARYAQSRYREIERVAV
                     VDWDVHHGNGTQGIFYDDPTVFFFSAHQYPWYPGTGTRGENGQGRGRGYTLNVPLKAA
                     TPAREHRRTFDAALEEIAAAFTPDLILISAGFDAHAADPLGQLLLEEEDFAEMTRTVK
                     QWAASACAGRIVSCLEGGYNLDALGASARAHVRALSEA"
     gene            1764..2168
                     /locus_tag="VGB76_14795"
     CDS             1764..2168
                     /locus_tag="VGB76_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100215.1"
                     /translation="MKRLIATLACLVLLATMIIVPDTSAQERRRRSRFGRKARTAAII
                     GGGAVAGGLIGGKKGAAIGAGGAGLYAFNRKAARRRFKGRTRTIGTVASGTALGAGVG
                     GAVGGKKAAIGGALAGAAGGALYRRSQRRRRN"
     gene            2319..2954
                     /locus_tag="VGB76_14800"
     CDS             2319..2954
                     /locus_tag="VGB76_14800"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY0100216.1"
                     /translation="MSHDEEQQRRSRVVVETPTARREEVYTRTTRTPEPDRGGHSTGT
                     VAIVALVAIAATALILFFWMNSGTDATNTNVNIATAPPTPIPTAPTPIFVQQPTPMQQ
                     QQPIIVQQPSTTQPAPVIIEQPAPATSTAPVVVPPANTGTDDATLESRLNKSFTDDAE
                     LVGITVTVVNGKATLIGSVKTQALKDRAARLANSVRGVRGVDNKLMVETAP"
     gene            complement(3046..3273)
                     /locus_tag="VGB76_14805"
     CDS             complement(3046..3273)
                     /locus_tag="VGB76_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100217.1"
                     /translation="MFSMQTSNAGDRATQVSFDDAGNAESVGDDGGDATLEDVAEREH
                     EYLRTRLREELQREPTEEELDEWLRKHTEGH"
     gene            complement(3296..3742)
                     /locus_tag="VGB76_14810"
     CDS             complement(3296..3742)
                     /locus_tag="VGB76_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100218.1"
                     /translation="MTVEWEPEATGTHQDHVIAHVVGASVLGYFRADDALHLSLDIGF
                     IWTIFPDAEMGLLPESLALRELNVSAEERASLLDEVQQLHDAEADHATLARVKPAPAG
                     CLIEEVRLLAKDELRRLEIQGEESALVVYASTVTGEMSVEELQMLE"
     gene            complement(3869..6463)
                     /locus_tag="VGB76_14815"
     CDS             complement(3869..6463)
                     /locus_tag="VGB76_14815"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY0100219.1"
                     /translation="MSTVIEPNAPQGNPIPAEARSRAAKQSYELGINARKVISKRYSL
                     KDAKGNPIEEWPDVVRRVVGHVSLAETDSEKRDEFYSRMSEIMLRREFVPNTPCLVNA
                     GKPNGQLAACFVLDVPDSIAGIMKTATDAAIIHQTGGGTGFTFEKLRPVGAMVRSTHG
                     VASGPVSFMNIFNTTTDTVKQGGVRRGANMGIMRVDHPDLLRFIHAKNDQTSLTNFNI
                     SVTVTDKFFEAVERKEWYQLSFDGEAWTEPVFDPLADGDYAIYRRPDGSTVTFRDKAA
                     FASADLSNTTREEPPRPGMVYAPDIWNRIVASAHRYAEPGVIFIDEVNRHNHMIKSMG
                     PIYATNPCAEQALHFNNSCNLGSVDVAKFYDPVERVDWESLGEAIRWSTRFLDNVIDV
                     CAWPLPEIDDVVKRTRPVGLGVMGFADLCLQLEVSYGSPESIDFMEELMSFVRREAWL
                     ESCRLGAERGVFPEYHPNKEAYDKFLRDDIGIPADMLTPRNYETTTIAPTGTISLVAE
                     TSSGIEPNFSWAYVRQDTLGKRTYVHTLAARALGLEVDQTDQASIDYASEYVVEHERE
                     LPDYFISAMSISAEQHIHVLAAAQRHVDNGVSKTCNGAADDTLESVDRLYRLGRELGC
                     KAVSYYRDGSRDSQVLSSMKGAEPKGEVACDPIIVNEAATAEMNLDETTAAENVVATE
                     TTRVAQPANAAATVQPVQIERPRELQGATWRIPFDGQNLYVTVNHDGRMILEVFATGP
                     ISGGVGLLASKMLRGAFDVREVARSLNKVIGTHSVWFNERLLTSPEQAVAECLLLTSR
                     RLQNQPDSARALAYAPPPAANAEAPAPQAKTNNNYSLISECPECRGQLEHASGCDFCR
                     DCGYSKCK"
     gene            6964..7914
                     /gene="hslO"
                     /locus_tag="VGB76_14820"
     CDS             6964..7914
                     /gene="hslO"
                     /locus_tag="VGB76_14820"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEY0100220.1"
                     /translation="MNEAQNMNNDSLVRATAADGGVRCMAAVTTNLVAEAVTRHQTSP
                     TVAAALGRTLTGTLLLGSGLKELDRLTVQIVCDGPVGGITAEANARGQVRGYARNPEA
                     NPDLNGKGKFDVSSVVGGGMFYVTHESGYEIGLYTDPYRGSVPIVSGELGEDFAYYLA
                     KSEQIPSAVTLGVYVRARETGETYVAAAGGLMIQVLPGADAKIIEAVEDSIRRGPHTT
                     TLIREGARPIDMLRAALGDLPFEVLEEKAVSFACTCSHERAVSLISSIDPTELEAILR
                     EDKGAAMTCHFCNETYRLDEAALENILASRREQQTSSGKR"
     gene            complement(8005..>8474)
                     /locus_tag="VGB76_14825"
     CDS             complement(8005..>8474)
                     /locus_tag="VGB76_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100221.1"
                     /translation="ANIQSLEQRWEDFVDLLDVYRRRKNHLRSINQQAVQNQLRQAER
                     AVENAPDERAKRIQQANVEILKRRLAAFGDLERSVKLVEGQLQSIENFFGLVNDQVVT
                     LPTPERVSALDFEQLSDSIEMTKQMLEETSDVLGTLDDHNRQAGNYDMLVSKQ"
BASE COUNT         1608 a         2581 c         2578 g         1707 t
ORIGIN      
        1 caagccgaag aagtcaacca ttgcggcgag cgtgatgagc aacacgatgg gcgtcagccc
       61 gacgaacagg taggtgatga cgagacgccg cctgacgcgc cacatcaagc gcgacttcaa
      121 ccagcgcaag ccctgaaagc cgtagtacgc agctgtgaaa tagatgagga cgagcgcgag
      181 cgtgaacagt tttccgacga tgccttcgcg gtagaagcgg ctgttgtgga tcatcaacca
      241 caaggcaaac agagccgccg acaggatcgc ggcgcgcggc atcacgcggc gcaaccacac
      301 gcgcagtcct tccatcctgt gcttcacatt ttccatcaat acttacaact cgcgacggtt
      361 ggcaattctc gaccttgtac gtctcgcaag ccggaggtgt tccagcaggt taagacgtgt
      421 atggaggctc atacgtttct acaaaattga gtgaagcact ccccttcttc acgcttcgct
      481 caacgcgcgc acgtgcgcac gcgccgacgc gccgagcgca tccagattgt agccgccttc
      541 gagacaggag acgatacgac ccgcgcacgc gctcgccgcc cactgtttga ccgtgcgcgt
      601 catctccgcg aagtcttcct cttccaataa caactggccg agcgggtcgg ctgcgtgcgc
      661 gtcgaagcct gcggagatca gtatgagatc gggcgtgaaa gcggcggcga tctcttcgag
      721 cgcggcgtcg aacgtgcgcc ggtgttcgcg cgccggagtc gcggccttga gcgggacgtt
      781 caaggtgtag ccgcgcccgc gcccctgccc gttctcgccg cgcgtgcccg tgcccggata
      841 ccaggggtat tgatgcgcgg agaagaagaa gacggtcggg tcgtcgtaga agatgccctg
      901 cgtgccgttg ccgtgatgca cgtcccaatc gacgacggcg acgcgctcga tctccctgta
      961 gcgcgactgc gcgtaacgcg cggcgacggc caccgcgttg aagagacaga agcccatcgc
     1021 attttcgctc gtcgcatgat gtccgggcgg tcgcgccgga acaaaagcgt tgcgcgcttc
     1081 gcccgccatg accgcatcca ctgcgcgaca cgccccgccc gccgcgcgca gagccgcatc
     1141 gagcgaacgc atcgaaatga tcgtgtcggc gtccagatag ccgcgccctt cgctcacggc
     1201 gcgttcgacg cgtttgaagt gctgcgtcgt gtgcgcggcc tgcacgtcgc cgcgcggcgc
     1261 aagcggggct tcgagttcca acagttgcga ccacaacccg tcgtcgccgc gcagagcctc
     1321 catcacggcg acataacgcg cggggctttc cgggtggttg tcgcccgtgt cgtgctcttc
     1381 gtaaaccgga tgatgaatga tggccgtctt catagacgcg gattatgcga gagcgcgcgt
     1441 gcgcggtcaa actggcaacc ggagtttaac ttataacggt ctgccgtaga gtgcttacgc
     1501 gcccccaggc acttcactcc ccatctctgc gctgaacttt tatgccactc tcatgcgccg
     1561 tatctgtatg gctatcatgc gcggctgtag tgaaaatctt ccggcgcaaa gttgtgttat
     1621 ggttaaagcg tcagtaattt tacgttttcg cgcggcgcac gcctcgccgc ttcgcggaat
     1681 aagatttgca cttgggagcg gcgtgatacg tctctcccat aaccaaattt cagcgaagaa
     1741 ttaattatca ggagacattt gacatgaaaa gattgattgc aactctggcg tgcctcgtcc
     1801 ttttggcgac gatgatcatc gtcccggaca catcggcgca ggaacggaga cgacgttcac
     1861 gcttcggacg taaagctcgc acggcggcca tcatcggcgg cggcgcggtc gcgggcggac
     1921 tcatcggcgg caaaaaaggc gcagccatcg gcgcgggcgg cgcgggtctt tacgctttca
     1981 accgtaaggc cgcgcggcgt cgtttcaaag gacgcacgcg caccatcggc acggtcgcca
     2041 gcggcacggc tctcggcgcg ggcgtcggcg gcgcggtcgg cggcaagaag gccgctatcg
     2101 gcggcgcgct cgcgggtgcg gcgggcggcg cactctacag acgcagccag cgtcgtcgtc
     2161 gcaactaacg aaattcacgt ttgggagagt ggccgctttt gacatccggc cactctcttt
     2221 tgcgtgcctg tgggcttcgt ggagcagcaa cgtcgaactt tttttccaac ctcgcgccat
     2281 ctttccgacc cgatcaatta tttaggagac gacccgttat gtcacacgat gaagagcagc
     2341 aacgcaggag ccgcgtagtg gtggagacgc cgacggcgcg gcgcgaggaa gtttacacgc
     2401 gcaccacgcg cacgcccgaa ccggatcgcg gcggacattc gacgggcacg gtcgccatcg
     2461 tcgcgctcgt cgccatcgcg gcgacggcct tgatcctatt cttctggatg aacagcggaa
     2521 ccgatgcgac caacaccaac gtcaacatcg ccacagctcc gccgacgccc atcccgaccg
     2581 cgccgacgcc gatcttcgtc cagcagccca cgcccatgca gcaacaacag cccatcatcg
     2641 tgcaacagcc ctcgacgacg cagcccgcgc ccgtcatcat cgagcagccc gcaccggcca
     2701 cctccacggc tccggtcgtc gtgcctccgg ccaacaccgg cacagacgac gcgacgctcg
     2761 aatccagact caacaagagt ttcacagacg acgcagaact cgtcggcatc accgtcaccg
     2821 tcgtcaacgg caaagcgacg ctcatcggct cagtcaagac tcaagccctc aaagatcgcg
     2881 ccgcacgcct cgccaactcc gtcagaggcg tgcgcggtgt ggacaacaaa ttgatggtcg
     2941 aaaccgcgcc ctaaaacgca aacggtgcgg taggaacgat gaacgcggaa tgaaagacag
     3001 gttgctttca ttccgcgttc atcgttccta ccgcatcgtt tcttctcagt gtccttcggt
     3061 gtgcttgcgc agccattcgt cgagttcctc ttcggtcggc tcgcgctgca actcttcgcg
     3121 gaggcgtgta cgcaaatatt cgtgttcacg ctctgcgaca tcctcaagcg tggcgtcgcc
     3181 gccatcgtcg cccacgctct ccgcatttcc cgcgtcgtca aaactcacct gtgtcgccct
     3241 gtctccggcg ttgctcgttt gcatggaaaa catttatcac ccctctctcc tgagttcact
     3301 ctaacatctg caactcttcg acgctcatct cgcccgtgac cgtcgaagca tagacgacga
     3361 gcgcggactc ctcgccctga atctcaagac gacgcagttc atccttcgcc agcaggcgca
     3421 cctcttcgat caggcacccg gcgggcgcag gcttgacgcg tgcgagagtt gcgtggtcgg
     3481 cctctgcgtc gtgcagttgt tgcacttcat cgagaaggct cgcgcgttcc tccgcgctga
     3541 cgttcaactc cctcaacgcc agcgactccg gcaaaagtcc catctcggcg tcgggaaaga
     3601 tcgtccagat gaagccgatg tcgagcgaca ggtgcagcgc atcgtccgcc ctgaaatagc
     3661 cgagcacgct tgccccgacc acgtgcgcga tgacgtggtc ctgatgcgtg cccgtcgcct
     3721 ccggctccca ctccacggtc ataagatctg tccgccaata tacacagcag tataaccagc
     3781 agcgcgctcc cgcccaaaga agaagggcgg ccaaaccatc gttcgccgcc ccttcttcat
     3841 caatctcaac cttctgtccg caatcgcctt atttgcactt cgagtagccg cagtcgcggc
     3901 agaagtcgca gccggaggcg tgttcgagtt ggccgcgaca ctccgggcat tcgctgatca
     3961 gtgaatagtt gttgttggtc ttcgcctgcg gcgcgggggc ttccgcgttg gcggcgggag
     4021 gtggggcgta ggcgagtgcg cgcgccgaat cgggttgatt ttgcaggcga cggctcgtga
     4081 gcaggagaca ctcggccacc gcctgttcgg ggctggtcaa gagtcgctcg ttgaaccaga
     4141 cggagtgcgt gccgatcact ttgttgaggc tgcgcgcaac ttcgcgcacg tcgaacgcgc
     4201 cgcgcaacat cttcgaggcg agcagaccga cgccgcccga gataggtccg gtggcgaaga
     4261 cttcgaggat catgcgcccg tcgtgattga ccgtgacgta gagattttgc ccgtcgaacg
     4321 ggatgcgcca cgttgcgccc tgcaactcgc gtgggcgttc gatctgaacc ggctgcaccg
     4381 tcgccgcagc gttagcaggc tgcgccacgc gcgtcgtctc agttgcgacc acattttccg
     4441 cagcagtcgt ctcatccaaa ttcatctcag ccgtcgcggc ctcgttgacg atgatcgggt
     4501 cgcaggcgac ctcgcccttc ggttccgcgc ccttcatgct ggagagcacc tgcgaatcgc
     4561 gcgagccgtc gcggtaatag ctgacggcct tgcagccgag ttcgcgcccc agacgataga
     4621 gacgatcaac cgattcgagc gtgtcgtcgg ctgcgccgtt gcaagtcttc gagacgccgt
     4681 tatcgacgtg acgctgcgcg gcggcgagca cgtggatgtg ctgctccgca ctgatggaca
     4741 tggccgagat gaaataatcc ggcagttcgc gttcgtgctc gacgacgtat tcggaggcgt
     4801 aatcaatcga agcctgatcg gtctggtcaa cttccagtcc gagcgcgcgt gcggcgagcg
     4861 tgtgcacgta ggtgcgcttg ccgagcgtgt cctgccggac gtaagcccag gagaaattcg
     4921 gctcgatgcc ggaggaggtt tcggcgacga gcgagatcgt gccggtcggc gcaatcgtcg
     4981 tcgtctcgta gttgcgcggc gtcagcatgt ctgcggggat gccgatgtcg tcgcgcagga
     5041 acttgtcgta ggcttccttg ttcggatgat attcgggaaa cactccgcgc tccgcgccca
     5101 gacgacacga ctccagccat gcctcgcgcc tcacgaaact catcaactct tccatgaaat
     5161 caatcgactc cggcgagccg taagaaactt cgagttggag gcagaggtct gcgaagccca
     5221 tcacgccgag gccgacgggg cgcgtgcgct tcaccacatc atcgatctcc ggcagcggcc
     5281 aagcgcacac gtcgatcacg ttatcgagga aacgcgtgct ccaacgaatc gcttcgccga
     5341 gcgattccca atccacacgc tcgaccggat catagaattt cgccacatca accgagccga
     5401 ggttgcagga gttgttgaag tgtagagcct gctccgcgca agggttcgtc gcgtagatcg
     5461 gacccatgct tttgatcatg tgattgtggc ggttcacttc gtcaataaag atcacgcccg
     5521 gctccgcgta gcgatgcgcc gaggcgacga tgcgattcca gatgtccggc gcatagacca
     5581 tgccggggcg cggcggctct tcgcgcgtcg tgttcgagag gtctgccgag gcaaacgcag
     5641 ccttgtcgcg gaaggtgacg gtcgagccgt cagggcgacg atagatggca tagtcgccgt
     5701 ccgccaacgg atcgaagacc ggctcagtcc aggcctcgcc gtcgaaactc agttgatacc
     5761 attccttgcg ttccaccgct tcgaagaact tgtccgtgac ggtaacggag atgttgaaat
     5821 tggtcagact cgtctgatcg ttcttcgcgt ggatgaagcg gagcaggtct ggatggtcga
     5881 cgcgcatgat gcccatgttc gcgccgcgcc gcacgccgcc ctgcttcacc gtgtcggtcg
     5941 tcgtgttgaa gatgttcatg aacgagacgg gaccggaagc gacgccgtgc gtcgagcgca
     6001 ccatcgcgcc gacggggcgg agtttctcga aggtaaagcc ggtgccgccg cccgtctggt
     6061 gaatgatggc ggcgtcggtg gcggtcttca taatcccggc gatggaatcg ggcacgtcaa
     6121 ggacgaagca ggcggcaagt tgaccgttcg gctttccggc gttgacgagg cacggagtgt
     6181 ttgggacgaa ctcgcgccgc aacatgatct ccgacatgcg gctgtaaaat tcgtcgcgct
     6241 tctccgagtc ggtttccgca aggctcacat gaccgacgac gcggcgcacc acgtccggcc
     6301 attcttcaat cggattcccc ttcgcgtctt tgagcgaata gcgtttgctg atcactttgc
     6361 gggcgttgat gccaagttcg tagctctgct ttgcggcgcg gctcctggct tcagcgggaa
     6421 tcggatttcc ttgtggagcg ttcggctcga tcactgtact catcttcaat ttcgcctttc
     6481 ccgcgctgca ttttttggcg cgcgagtcct catgtgtgca ttgcaatccg gcgcgggctg
     6541 cacagggcgc gccgttgatc aagtgtgtta gcggaaactg cggctggctt gaacgttaag
     6601 cgaaagatat ccttgccgaa cgtgccccgg gagtcgcggc gtttcggcct tctgacgttc
     6661 tcgtcatcaa gcggagcgca gtatacatag ctgatatcgg gctgtcaata cccagcccca
     6721 atatattgta tcaacttttc ttttcagcgg catatggtgg gcactttaag agtttacctt
     6781 aacccaactt tatgcagatt tgaggcatca aaacagttct ttttgccccg ctttgacgcc
     6841 cggcgcggga agattccgag acggtaaaaa aattttcggg gctggcggaa ggcgcgcggt
     6901 tgagcgaacc ttttttgtcc tgaaaggcgg gttggcgata gaatattttt aaggggaata
     6961 attttgaacg aagcacaaaa catgaacaat gattccctcg tgcgggcgac ggctgccgat
     7021 ggcggcgtgc gctgcatggc ggcggtgacg acgaaccttg tggccgaagc ggtgacgcgt
     7081 catcaaacct cgccgacggt ggcggcggcg ctcggtcgca cgttgacggg cacgttgctg
     7141 ctcggctcag gactcaagga actcgaccgc ctgaccgtac agatcgtgtg cgacgggccg
     7201 gttggaggca tcacggcgga agcgaacgcg cgcgggcagg tgcgcggcta cgcgcgcaac
     7261 cccgaagcga atcccgactt gaacggcaag ggaaagttcg acgtgagttc tgtcgtcggc
     7321 ggcgggatgt tttacgtcac gcacgagtcg ggctatgaga tcgggttgta cacagacccc
     7381 tatcgcggct ctgtgcccat cgtgtcgggc gagttgggcg aggacttcgc ttattacctc
     7441 gccaagtcgg aacagattcc ctctgcggtg acgctcggcg tctatgtgcg cgcgcgcgaa
     7501 acgggcgaga cttacgtggc ggcagcaggc ggcttgatga tccaggtctt gccgggggcg
     7561 gacgcaaaga tcatcgaggc ggtcgaagat tcgatccggc gcggcccgca caccaccacg
     7621 ttgatccgcg aaggcgcgcg tcccattgac atgctccgcg ccgcgctcgg cgatcttccg
     7681 ttcgaggtgc tggaggagaa agcggtcagc tttgcctgta cctgctcgca tgaacgcgcc
     7741 gtctctttga tctcttcgat tgatcccacg gagttggagg cgattttacg cgaagacaaa
     7801 ggcgcggcca tgacttgcca cttctgcaac gagacataca ggctggacga agcggcgttg
     7861 gagaatattc tggcatcgcg acgtgaacaa caaacatcga gcggcaagcg gtaaacggcg
     7921 cgggggttga tcaggaaaag cgaaaggcag tcgaggtgag tttccttcga ctgcctttcg
     7981 ctttttgccg gtcgtttgcg cggcttactg tttcgagacg agcatgtcgt agttgcccgc
     8041 ctgccggttg tgatcgtcga gcgtgccgag tacgtcggat gtctcttcga gcatctgctt
     8101 ggtcatctcg atggaatcgc tgagttgctc gaagtcgagc gcggagacgc gttcgggcgt
     8161 cggcagcgtg acgacttgat cgttgacgag gccgaaaaag ttttcgatgg actgcaattg
     8221 tccttcgacc agtttcacgg agcgttcgag gtcgccgaag gccgcgagcc gtcgcttcag
     8281 aatctccacg ttggcctgct ggatgcgttt ggcgcgttca tcgggggcgt tctccaccgc
     8341 ccgttcggcc tgccttaatt gattctgcac ggcctgctgg ttgatcgagc gcaggtgatt
     8401 tttgcggcgg cgatagacat cgagcaggtc aacgaaatct tcccagcgtt gttcgaggct
     8461 ttgaatgttc gcgg
//
LOCUS       DASWNS010000427        13424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_484863, whole genome shotgun sequence.
ACCESSION   DASWNS010000427 DASWNS010000000
VERSION     DASWNS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13424
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484863"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2173
                     /locus_tag="VGB76_14830"
     CDS             <1..2173
                     /locus_tag="VGB76_14830"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0100222.1"
                     /translation="LVSLFVRSSSPLSLQPTPITSIETLSLERKIKMHEAREAIINRL
                     APTIHTKIDLDKFLQAVVSELGQMMNVDRCDVIQLASEGELRISHEWRASEDIPSSLG
                     MSVPIDFRQLAGRFNLAQPIRIDDTAAPDLDPKVSLLPASLNTRSLLVVPVVLAGDVL
                     GLVGMHTVRAPRKWLDEEVSFLQSIARQIAVGYQYARLYTDQQRESHRTRALLEIANT
                     LNARSDFNEVTSLVLERALALVGADYCALGVMNPTGKAVSLAAFKAAPHAATEGVRGL
                     IKEHGMSIDITDYPAIQELFKEGKTLQVFDTTLPPPVRFVFNATLGGHAALVAPVHIG
                     GQTFGLLGLVWSAARESFDEHETALVEGIADQLGTALERDQLSAEVMRLRTVLHQRRA
                     EDRIIGQAPAIRRAIELALNVADTQTSVLIQGESGTGKELLANLIHYNSHSKERTYIK
                     LNCGAIPETLLESELFGHERGAFTDARTQRRGKFEEADGGTLFLDEIGEMSLSAQVRL
                     LRVLQDGEFTRVGGNEVIKADVRVIAASNVDLERAVEAGTFRRDLFYRLSVFPVRLPP
                     LRERAEDVHPLVIHFLEHYKQKTGRYISGISKDALEALTTYDWPGNVRELENAIERAV
                     IIASGRQIEVDDLPEAIGRPLLEARARTRTERTRASAEGRHIKLELDIPSTLEEIERR
                     AIEATLDYTGGDKTRTARTLGIGRKTLYRKLLGYQSDDSKI"
     gene            2340..2690
                     /locus_tag="VGB76_14835"
     CDS             2340..2690
                     /locus_tag="VGB76_14835"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HEY0100223.1"
                     /translation="MKPEEKQWWKDKQTSDYNKARDSWHQQQMSRDKPAKGGCFVATA
                     AFGDYNAPEVVYLSAFRDESLSRSVLGRGFIRAYYAVSPRFASVIEKSSFLRLAVRKL
                     FLQPIIFLLRLIRY"
     gene            2733..3167
                     /locus_tag="VGB76_14840"
     CDS             2733..3167
                     /locus_tag="VGB76_14840"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY0100224.1"
                     /translation="MENDISYEQYLDSLPAGRRVEIERVWGLVREHIPEGYTEEVGEK
                     FLSFKAGADWYVALANQKNYISLYLIPLYMFPEMKAKFDAAASKKLKCGKSCINFQRA
                     EELPLEVVGEIVGSYDAQSYAGQVQQVRGAERPPRKKGKGTV"
     gene            3238..3804
                     /locus_tag="VGB76_14845"
     CDS             3238..3804
                     /locus_tag="VGB76_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100225.1"
                     /translation="MPMTGSPRAGAKYAYGFATEALTGGREIRGHSGYRVLRRKRPDL
                     DATQRAHAVDWLITLSILAVGVLLLVLGASGRITKNLAVVYSLGAGSALYAVYDLYRF
                     ARPLAFPFSENLWLYEHLVKMLGGYFGAVVAFSGSVLMLLPEPWRQLWATSFGQTLAV
                     VLVIYYRRKLRARPSRNQPAAHRLAANQ"
     gene            4002..4451
                     /locus_tag="VGB76_14850"
     CDS             4002..4451
                     /locus_tag="VGB76_14850"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEY0100226.1"
                     /translation="MSKQGVQTYRQRRGGVGGDESGANLSTQVTCFLLGGAVGALAAL
                     LFAPKSGRELRGDIADVTRQGVDRARDKANEYYEVSRERATELYSTASSKAGEVAGAA
                     RQAATRRGENLSAAIDAGKRAYAEEKRRTENTNILNPAPTYYEPDKS"
     gene            4559..5128
                     /locus_tag="VGB76_14855"
     CDS             4559..5128
                     /locus_tag="VGB76_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100227.1"
                     /translation="MCLAFQMSVNDPAFWFMLIVAVAFVAISVAIVMMALAVRRVVVM
                     VGNLERRAEPLLERVASLSEQVKEIAAQGKEVAEQVTLMSTHLATASVHFSESSALIK
                     EEVRELKQLVGYTAVTARDKVEQVSRTVDQAQRQFTATTFFIHTKLVEPARELAAIMA
                     GVRRGLEVLVAPAPKQIDQTYGEDEMFIG"
     gene            5247..8225
                     /gene="ileS"
                     /locus_tag="VGB76_14860"
     CDS             5247..8225
                     /gene="ileS"
                     /locus_tag="VGB76_14860"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY0100228.1"
                     /translation="MTTETPLELKKTVNLPKTNFAQKANLAQAEPARLKKWSELNLYQ
                     LIREARRGREKFILHDGPPYANSDIHLGTALNKIVKDFVVKSRTMFGYDSPYVPGYDC
                     HGLPIELYVDKKLGAKKANMPALSVRRACRQHATDALKRQTKDFQRLGILGLWENPYL
                     TMSNQYEAETARLFGRFVERGYVYKGARPVYWCVYDQTALAEAEVEYREHTSPSVYVK
                     FPLASDPSELDPALAGRRVFFLIWTTTPWTLPANLAIAVNPNFEYAAIENGEEVYVVA
                     SELAGAVAEKCGLDAPRVIARFAGAKLDRLECRHAWLERRSLVILGDHVTLGGEADAE
                     TELDVKEARDKSATGKAGTGCVHTAPGHGHDDFVIGKQYGLEIYCPVDHAGRFTAEVE
                     HFAGERVFEANAKIVQFLAERGALLFTEEYRHRYPHCWRCKNPVIFRATPQWFISLDA
                     HESEPPVEHDEDGRPLSNYTENETDPPVSLRTGALREVAHNVRWIPAWGEDRMRNMLK
                     GRPDWCVSRQRVWGVPIPVFYCDACDAPIADRSVIKHVADIFERESSDAWYAREANEL
                     LPENFACPNCNSSAFKKETDILDVWFDSGSSSVAVLEHYEELRYPADVYLEGGDQYRG
                     WFNSSLMVGLAAHDRAPYRAVLTHGWVVDGQGKAMHKSGGNAVSPNDVIKESGAEIIR
                     LWCASSDYHEDMRCSSEILQRVSDGYRKMRNTARFALGNLDGFDPSRDAVAYEEMEEI
                     DRWALAELDAVIERVRAAYEAYEFHTVYHTLYNFCTVTLSARYFDIIKDRLYTAAPRS
                     HARRSAQTALHAIADALARLLAPILVFTADEIWENIPRAGGESDPAASLRSVHLAEFP
                     APLNLRDEELAARWSQLFAVREEVLAALEKERVAKVIGSSLEARVTLAAAGQTYELLK
                     GYEDQLRYLFIVSQVDVVESDVPPADLSFATGVSRAGGEKCERCWNYSTRVGESARYP
                     TVCERCVEALAEIEREGGAA"
     gene            8222..8740
                     /gene="lspA"
                     /locus_tag="VGB76_14865"
     CDS             8222..8740
                     /gene="lspA"
                     /locus_tag="VGB76_14865"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY0100229.1"
                     /translation="MKARELSWRIWYLLAAFGIYMADQMSKAWAAQRLRLGQDKTIID
                     GFLDLAYAENTGVAFGQLQDGGAWGRYALAALAGAAAVAVLTYFFRTPRSDDRTLGAC
                     ALLLAGIAGNLTDRLRLGYVIDFIHVYAGNYHWPTFNVADASICVGAALFALDLILEG
                     RKQKQHSTVNSQ"
     gene            8890..9726
                     /gene="lgt"
                     /locus_tag="VGB76_14870"
     CDS             8890..9726
                     /gene="lgt"
                     /locus_tag="VGB76_14870"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY0100230.1"
                     /translation="MYPEIFRIGDFPINTYGVLLALAFLAALLVAARLGARDGLPRER
                     LFDLGLWLLLAAIVGSKLLLLVVEPEYRAAPLRLLSLDFLRSGGVFYGGFLAAVLTAY
                     FLVRRYRLPWWRTADAFAPGIALGQAIGRQGCFAAGCCWGKPTTLPWGVRFSELGHQV
                     TGVPIDAHLHPTQLYESFAAFLFFALLIWLHRRKSFDGQVLLVYGVLYGATRFIIEFF
                     RDDPRGDIFGLTSLTHLSTSQLISLLVGLTSLVILVIRWRKASNDPLPEGRKVVGTTA
                     RA"
     gene            9730..10752
                     /locus_tag="VGB76_14875"
     CDS             9730..10752
                     /locus_tag="VGB76_14875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0100231.1"
                     /translation="MNDPLQDKPDDGDDAETTFPAGVPEQEAATLAFTVEEGEAGARL
                     DAFLAARVEGVSRSRLKQSIEDGDVLVAGRTALKGSYKLRAGEQVELEMPAPPACDFM
                     PEEIPLKIVYEDAWLVVLDKPAGLVVHPAAGVSTGTLANALAFHFRQLSAHAGATRPG
                     IVHRLDRDTSGLMVVAKTERAHEHLSDQFRAREVFKSYVALVHGRVREESGRIEEPLA
                     RDPRTRTRMAVRSGGRPALSLYRIRRAYERFTLLDVQIKTGRTHQIRVHLAHLKHPVV
                     GDKVYNGGRDTTVADVRVRAAIRAMGRQFLHAEQLGFRHPHTGEDLRFDAPLPTELSQ
                     MLAALG"
     gene            10988..12028
                     /locus_tag="VGB76_14880"
     CDS             10988..12028
                     /locus_tag="VGB76_14880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0100232.1"
                     /translation="MRTAFYSLLFALALVLSGALALDASVSAQSSNQQPRPAAPSPRP
                     TPPPQDQDDVQGGTVFTREVQLPITVMDKKDQMVSGLKAGDFIIYEDKQPQQIASFRD
                     ESAGTPIYVAVLMDISSSAAAKLRFEKESAKDFLYTVVRVRKDKAAFATFDEDVNLLQ
                     DFTDKLDKLDTAVDSVKKPGTTTALYDAVWRMCDEKMRGVTGRRVIVVITDGEDTSSR
                     ARLEEAIEMAQRTETIIFAISTKAGFSSVVPGVEAGTVKDDDDKRLDKLADATGGKAF
                     FIGDRLDLERAFTKVAKELRGQYIVTYRPTNKNYDGNYRRIEVKLAAERDGLRVRTRR
                     GYTAQPIRANNE"
     gene            12134..13261
                     /locus_tag="VGB76_14885"
     CDS             12134..13261
                     /locus_tag="VGB76_14885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0100233.1"
                     /translation="MFDRSQFMRVLSFILAFSVTFGGALAGARAQQQQQQPAQQPAPA
                     RTPPAQRDDNTTLDDDEIERVDTSLTNVIFTAVDRDKRFLSNLKQEDIRVLEDGVPQQ
                     IFTFQRSIDLPLTLAIVIDTSISQERTLPEEKRAARAFVDAVMRASKDEVAVLSFTGD
                     STLEQGLTGNPSRVRSAIERVEFVPPSGYVGRGVLVRGGTPPISGDNQSTAGSTAIWD
                     ALYATCAEVMTESSDKTRRAIILLSDGEDTSSTLKLDEAVQEALKTETMIFAIGIGDS
                     FNYSGVDEGSLRKITERTGGRAYFPRSEEDLRNAFEQIQRELREQFLVAYSPTNKRRD
                     GSYRKLQIEVINPELRNRNIKLSYRQGYFAKSDAAPAKRRR"
BASE COUNT         2672 a         4251 c         3998 g         2503 t
ORIGIN      
        1 tctcgtgagt ttgtttgtaa ggagcagcag ccctttgtct ttgcagccta cgcccatcac
       61 gagcatcgag acgctctcgc tcgaacgcaa gatcaagatg cacgaagcgc gcgaagcgat
      121 catcaatcgc ctcgcgccca ccattcacac caagatcgat ctcgacaaat tcctgcaagc
      181 cgtcgtaagt gaactcggac agatgatgaa cgtggatcgc tgcgacgtga tccaactcgc
      241 ttccgaaggc gagctacgca tcagccacga atggcgcgcg agcgaagaca tcccttcgag
      301 cctcggcatg tccgtgccca tagactttcg ccaactcgcc ggacgcttca atctcgcgca
      361 gcccatccgc attgacgaca ccgccgcgcc cgatctcgat ccgaaggtta gtctcctgcc
      421 cgcgagcctc aacacgcgct cgctcttagt cgtgcccgtc gtcctcgcgg gcgacgtgct
      481 cggactcgtc ggcatgcata cggtgcgcgc gccgcgcaag tggctcgacg aagaagtctc
      541 ctttctacag tccatcgcgc gccagatcgc ggtcggctat caatacgcgc gtctctacac
      601 ggatcagcag cgcgagtcgc atcgcacgcg cgcgctgctg gagatcgcaa acactctgaa
      661 cgcgcgctcg gacttcaacg aagtcacttc actcgtcctc gaacgcgcgc tcgccctcgt
      721 cggcgcagac tattgcgcgc tcggcgtgat gaacccgacg ggcaaagccg tgagcctcgc
      781 cgctttcaag gccgcgccgc acgccgccac cgaaggcgtg cgcggcctca tcaaagagca
      841 cggcatgtca atcgacatca cggactatcc cgcgattcag gagttgttca aagaaggcaa
      901 gacgctgcaa gtcttcgaca cgacgctccc gccgcccgtg cgcttcgtct tcaacgcgac
      961 gctcggcggc cacgccgcgc tcgtcgcgcc cgtccacatc ggcgggcaga ccttcggact
     1021 cctcggcctc gtctggagcg cggcgcgcga aagtttcgac gaacacgaga ctgcgctcgt
     1081 cgaaggcatc gcggaccaac tcggcacggc tctggagcgc gaccaactct ccgccgaagt
     1141 gatgcgcctg cggacagtcc tccaccaacg ccgcgccgaa gaccgcatca tcgggcaagc
     1201 ccccgccatc cgccgcgcca tcgaactcgc cttaaacgtc gcagacacgc aaacctccgt
     1261 gctcattcaa ggcgaatccg gcacgggcaa agaactctta gccaacctca ttcactacaa
     1321 ctcgcacagc aaggaacgca cctacatcaa actcaactgc ggcgcgattc ccgagacgct
     1381 cctcgaatcc gaactcttcg gccacgaacg cggcgcgttc acggacgcgc gcacgcagcg
     1441 gcgcggcaag tttgaagagg ccgacggcgg cacgctcttc ctcgatgaga tcggcgagat
     1501 gagtttgtct gcgcaggtgc gtctgctgcg cgtgttgcag gacggcgagt tcacgcgcgt
     1561 cggcggcaac gaagtcatca aggctgacgt gcgcgtcatc gccgcgtcga atgtcgatct
     1621 cgaacgcgcg gtcgaagcgg gcacgttccg gcgcgatctc ttttatcgtc tctcggtctt
     1681 ccccgtgcgc ctgccgcctc tgcgcgagcg cgccgaagac gtgcacccgc tcgtcataca
     1741 ttttctcgaa cactacaagc agaagaccgg acgctacatc tccggcatct ccaaagacgc
     1801 gctcgaagcc ctcaccactt acgactggcc gggcaacgtg cgcgaactcg aaaacgccat
     1861 cgagcgcgcc gtcatcatcg cgtcgggtcg ccagatcgaa gttgacgatc tgcccgaagc
     1921 catcggtcgc ccgctcctcg aagcgcgcgc acgcacgcgc accgagcgca cgcgcgcctc
     1981 cgccgaaggc cgccacatca aactcgaact cgacatcccc tccacgctcg aagagatcga
     2041 acgccgcgcc atcgaagcca ccctcgacta caccggcggc gacaagacac gcaccgcacg
     2101 caccctcggc atcggacgca aaaccctcta ccgcaaactg ctcggctatc aaagcgacga
     2161 ttcgaagatc tagtcgctta tcacgtaatt aaagagtgag ccgcagtcta agcagtagtt
     2221 atgcttttat atcttgctaa cgatttgcgc ttttggcagt cacgaacggg gactcaatct
     2281 ggcctctgat ttaaatggcg cattaacata ctgaccccaa attttggagg aggaagatta
     2341 tgaaacctga agaaaagcag tggtggaaag acaaacaaac gagtgactac aataaggcaa
     2401 gggatagttg gcatcagcag cagatgtcac gcgacaaacc cgctaagggt ggatgctttg
     2461 tcgcaactgc cgctttcgga gactataacg cgccggaagt agtttatctt tcagcatttc
     2521 gtgacgaatc attgagccgt agcgtcttgg gacgtggatt tattcgtgcc tactacgcag
     2581 tctcgcctcg attcgcatcg gttattgaga agtcgagctt tttgcggctg gcggttcgca
     2641 agttattctt acaaccgatc atatttttgt tgcgactaat tcgttattga agttttcggt
     2701 acgctcgaaa agcattcagg aaggacacgt cgatggagaa cgatatttca tacgagcagt
     2761 atcttgatag cttgcccgca gggcggcggg tggagattga gcgcgtgtgg gggttggtgc
     2821 gggaacatat tcccgaaggt tacacggagg aagtggggga gaagttcttg agtttcaagg
     2881 caggcgcgga ttggtacgtc gcgctggcga atcagaagaa ctacatcagt ctctacttga
     2941 tcccgctcta catgtttccg gagatgaaag cgaagttcga cgctgccgct tcgaagaaac
     3001 tcaagtgcgg gaaaagctgc atcaattttc agcgcgccga agagttgccg cttgaggtgg
     3061 ttggagagat cgttggaagt tacgacgctc aatcctacgc ggggcaggtg cagcaggtgc
     3121 ggggggcgga gaggccgccg cgaaaaaaag gcaaaggcac agtctgagcg catgctgcgt
     3181 gattgtcgtt gagccgctga gagatgattc cgttggcgac tcgtcacgag cgcgaaggtg
     3241 ccgatgacgg gttcgccgcg agcgggtgca aaatatgctt acggatttgc gaccgaggcg
     3301 ttgacgggcg ggcgtgagat acgcgggcac agcggctatc gcgtcctgcg tcgcaaacgt
     3361 cccgacctcg acgccactca aagagcgcac gccgttgact ggctcatcac gctctcgata
     3421 ctcgccgtcg gagtgttgct tctggtgctc ggcgcgtcgg ggcgcatcac gaagaacctg
     3481 gccgtagtct attcgctcgg cgcggggagc gcgctgtatg ccgtctatga tctatatcgt
     3541 ttcgcgcgcc cgctcgcctt tcctttctcg gaaaacctct ggctctacga gcatctcgtc
     3601 aagatgctcg gcggttattt cggcgcggtc gtggcctttt cgggcagcgt cctcatgctc
     3661 ctgcccgaac cgtggcgtca actctgggcg acctcgttcg gccagacgct cgccgtcgta
     3721 ctcgtgatct actaccggcg gaaactgcgc gcgcgcccct ctcgcaatca acccgccgct
     3781 catcgtctcg ccgccaatca ataagaagcc gcacgtcgaa ccgtcgaggc acgtcgccgc
     3841 aagaagtttt tccgccttat taacctcttg ttaagaagcg tttcttgcgc cgcttcgcgc
     3901 ctctttgtta tagtttttgc gcgggacgat agacattaag tttttcacac actaaacgtc
     3961 gaagcgacgg cgtttcgatt cgcagaaaag gggtcacgtt gatgtccaag caaggtgtac
     4021 agacttatcg ccagcggcgc ggcggcgttg gcggcgacga aagcggggcg aacctctcca
     4081 cgcaggtcac ctgcttcctg ctcggcggcg cggtcggcgc gctcgcggct ctcttgtttg
     4141 cgccgaagtc cgggcgggaa ctgcgcggcg acatcgccga cgtgacgcgg cagggcgtag
     4201 accgtgcgcg cgataaagcc aacgagtatt acgaggtttc gcgcgagcgc gcgaccgaac
     4261 tttattccac ggcttcgagc aaagcgggcg aagtggcggg ggcggcgcgc caggctgcca
     4321 cacggcgcgg cgaaaacctg tcagccgcca tcgacgcagg caaacgcgcc tacgccgaag
     4381 agaaacgccg caccgaaaac accaacattc tcaatcccgc gccgacttac tacgagccgg
     4441 ataagtctta aagaacgcgt cgctcggatc gccggaggcg ggttgagaag cagagacgat
     4501 taactatcaa ctgcttgttt tcccatccgc atttcgtagt ccgaagtccg aaacctctat
     4561 gtgcctagct ttccaaatga gcgtaaatga tccggcgttc tggttcatgt tgatcgtcgc
     4621 cgtggccttc gtcgctatct ccgtcgccat cgtgatgatg gcgctcgcgg tgcggcgcgt
     4681 cgtggtgatg gtcggcaatc tcgaacggcg cgcggagcct ctgctggagc gcgtggcgtc
     4741 gctgagcgag caggtcaaag agatcgcggc gcagggcaaa gaggtggccg agcaggtgac
     4801 gctgatgtct acgcatctgg cgacggcctc cgtacacttt tccgaatcct ccgcgctcat
     4861 caaagaagag gtgcgcgaac tcaaacaact cgtcggctat acggctgtga cggcgcgcga
     4921 caaggtcgaa caggtgagcc gcacggttga tcaggcgcag agacagttta ccgccactac
     4981 ctttttcatc cacacgaagc tggtcgaacc ggcgcgcgag ttggccgcga ttatggcggg
     5041 cgtccggcgc ggtctggaag tgctggtggc gcccgccccg aaacagattg accagaccta
     5101 cggagaggac gagatgttca tcggctgaag agcgcgcgcg tccgactcca catttccgaa
     5161 gtcccaagtt caaggtcaga ctttggactt gggactttcc ggttttatgt tgcaagattt
     5221 gcattgaaaa attttagaaa accgttatga ctaccgaaac accgctcgaa ctcaagaaga
     5281 cagtcaattt gccgaagacc aacttcgcgc agaaggccaa cctcgcgcag gccgaacccg
     5341 cgcgcctcaa aaagtggtcg gagctaaacc tttatcaact gatccgcgaa gcgcggcgcg
     5401 ggcgcgagaa gttcatcctc catgatggac ctccctacgc caactccgac atccacctcg
     5461 gcaccgcgct caacaagatc gtcaaagact tcgtcgtcaa atcgcgcacg atgttcggct
     5521 acgattcgcc ctacgtcccc ggctacgact gtcacgggct gcccatcgaa ctctacgtgg
     5581 acaaaaaact gggggcgaag aaggcgaaca tgcccgccct gagcgtgcgc cgcgcgtgcc
     5641 gccagcacgc gaccgacgcc ctcaaacgac agacgaaaga tttccagcgg ctcggcatac
     5701 tgggtttgtg ggagaaccct tatctcacga tgtcgaatca atacgaggcg gagacggcgc
     5761 ggctcttcgg tcgcttcgtc gaacgcggtt acgtctacaa gggcgcgcgt ccggtctact
     5821 ggtgcgtcta cgatcagacc gcgctggcgg aggcggaggt cgaatacagg gagcacacga
     5881 gtccttccgt ctacgtgaag tttccgctgg cgagcgatcc gtctgaactc gaccccgcgc
     5941 tcgccgggcg cagagtgttc ttcctcatct ggacgacgac gccctggacg cttcccgcga
     6001 acctcgccat cgccgtcaac ccgaactttg aatacgcggc gattgaaaac ggcgaagagg
     6061 tttacgtggt cgccagcgaa ctggccggag ccgtggcgga gaagtgcggg cttgatgcgc
     6121 cgcgcgtcat cgcgcgcttc gcgggcgcaa aactcgaccg cctcgaatgt cgccacgcgt
     6181 ggcttgaacg caggtcgctc gtcatcctcg gcgaccacgt gacgctcggc ggcgaggccg
     6241 acgcggagac ggaactcgac gtgaaagagg ctcgtgataa gtctgcgacg ggcaaggccg
     6301 ggacgggctg cgtgcacacc gcgcccggac acgggcacga cgattttgtg atcggcaagc
     6361 aatacggtct ggaaatctac tgccccgtag accacgccgg acgcttcacc gccgaagtcg
     6421 aacacttcgc gggcgagcgc gtgtttgaag cgaacgcgaa gatcgttcag tttctcgcag
     6481 agcgcggcgc gctcctgttc acagaagaat acagacaccg ctacccgcat tgctggcgtt
     6541 gcaagaatcc ggtcatcttc cgcgccacgc cgcagtggtt catctcgctc gacgcgcacg
     6601 aaagcgagcc gcccgtcgaa catgacgagg acggtcgccc gctctcgaac tacacggaga
     6661 acgagacaga cccgcccgtc tctctgcgca cgggcgcgct gcgcgaagtg gcgcacaacg
     6721 tccggtggat tccggcgtgg ggcgaagacc gtatgaggaa catgctgaaa gggcgtcccg
     6781 actggtgcgt gtcgcgccag cgcgtgtggg gcgtgccgat cccggtcttc tactgcgacg
     6841 cgtgcgatgc acccatcgca gaccggtctg tgatcaaaca cgtcgcggat attttcgagc
     6901 gcgaatcatc ggacgcgtgg tatgcacgcg aggcgaacga gttgctgccg gaaaacttcg
     6961 cctgcccgaa ctgcaactct tccgcgttca agaaagagac ggacattctc gatgtctggt
     7021 tcgacagcgg ctcgtcgtcg gtcgccgtgc tcgaacacta cgaagagtta cgctatcccg
     7081 cagacgtgta tctcgaaggc ggcgatcaat atcgcggctg gttcaactcg tctctgatgg
     7141 tggggctcgc cgcgcacgac cgcgctcctt atcgcgccgt gctcacgcac ggttgggtgg
     7201 ttgacggtca gggcaaggcg atgcacaagt caggcggcaa cgccgtctcg ccaaacgacg
     7261 tgatcaagga gtcgggcgcg gagatcatac gcctctggtg cgcctcatcc gactatcacg
     7321 aagacatgcg ctgctcgtcc gaaatcctgc aacgcgtctc ggacggctac cggaagatgc
     7381 gcaacaccgc gcgcttcgcc ctcggcaacc tcgacggctt cgacccgtcg cgcgacgcgg
     7441 tcgcttatga agagatggaa gagatcgacc gttgggcact cgcggaactc gacgccgtta
     7501 tcgaacgggt gcgcgcggct tacgaggctt acgagtttca caccgtctat cacacgctct
     7561 acaacttctg caccgtgacg ctctcggcgc gctacttcga catcatcaag gacaggctct
     7621 acaccgccgc gccccgctcg cacgcgcgac gctcggcgca gaccgccctg cacgccatcg
     7681 ccgacgccct cgcgcgcctg ctcgcgccga tccttgtttt caccgcagac gaaatctggg
     7741 agaacatccc gcgcgcaggt ggcgagagcg accccgccgc ctcgctccgg tcggttcatc
     7801 tcgcggagtt tcccgcgccg ctcaatttac gcgatgagga gttggccgcg cgctggtcgc
     7861 agttgtttgc ggtgcgcgaa gaagttctgg ctgcgcttga gaaggagcgc gtggcgaagg
     7921 tgatcggcag ttcgctcgaa gcgcgcgtga cgctcgccgc cgcagggcag acttacgaac
     7981 tgctcaaggg ttacgaagat caattgcgct atctgttcat cgtctctcaa gtggacgtgg
     8041 tcgagtcgga tgtgccgccg gcagacttat ctttcgcgac cggcgtttca cgcgccgggg
     8101 gcgagaagtg cgagcgttgc tggaactact ccacgcgcgt cggcgaatcg gctcgctacc
     8161 cgacggtgtg cgaacgctgc gtcgaagctc tggccgagat cgaacgggaa ggaggcgcgg
     8221 cgtgaaggcg cgagagttaa gttggcgcat ctggtatctg ctcgcggctt tcggcatcta
     8281 catggcggat cagatgtcga aggcctgggc ggcgcagagg cttcgtctcg gacaggataa
     8341 gacgatcatc gacggctttc tcgatctcgc ttacgcggag aacacgggcg tcgccttcgg
     8401 gcagttgcag gacggcggcg cgtggggtcg ctatgcgctg gccgcgctcg ccggggctgc
     8461 ggctgtcgcc gtgctcacct atttcttccg cacgccgcgc agcgacgacc gcaccctcgg
     8521 cgcgtgtgcg ctcctgctcg cgggcatcgc gggcaactta acggatcgcc tgcggctcgg
     8581 ctacgtcata gacttcatac acgtctacgc gggcaactat cactggccga ccttcaacgt
     8641 cgccgacgcc agcatctgcg tcggcgcagc cctcttcgcg ctcgacctga ttctggaagg
     8701 caggaagcag aagcagcact cgaccgttaa cagccagtag cgttctattc atggtttaag
     8761 gagaagacat gcggcacagg agaaagcaga tagcctgtcg gatgtcgcaa tcgaattctc
     8821 attcgctcag gcgaggcagt aatccgggcg cgttcgcatc taatagttga cggctggcaa
     8881 atatctcaga tgtatccaga aatttttcgc atcggcgatt ttccgattaa cacttacggc
     8941 gtgctgctgg cattggcctt tctcgccgcg ctgttggtgg cggcgcgtct gggcgcgcgc
     9001 gacgggttgc cgcgcgagcg tctgtttgat ctggggctgt ggttgttgct ggcggcgatt
     9061 gtcgggtcga agctcttgtt gctggtggtc gaaccggagt atcgcgctgc gcctctgcgc
     9121 ctgttgtcgc tcgatttcct gcgttcgggc ggagtctttt acggcggctt cctcgcggcg
     9181 gtcttgacgg cttactttct ggtgcgacgt tacaggttgc cctggtggcg tacggcggac
     9241 gcgtttgcgc ccggcatcgc tctcggtcag gccatcgggc gacagggctg tttcgccgcc
     9301 ggatgttgtt ggggcaagcc gaccacgctg ccgtggggcg tgcgcttctc ggaactcggc
     9361 catcaggtga cgggcgtgcc catcgacgcg cacctccacc ccacgcagct ttacgaatcc
     9421 ttcgccgcct ttctcttctt cgcgcttctc atctggctgc accgccgcaa gtctttcgac
     9481 ggtcaggtgc tgctcgtcta cggcgtgctc tacggggcga cgcgcttcat catcgagttc
     9541 ttccgcgacg atccgcgcgg cgacattttc ggtctaacgt cgctcacgca tctctccacg
     9601 tcgcagttga tcagcctgct cgtcggcctc acgagtctcg tcatactcgt catccgctgg
     9661 cgcaaagcat cgaacgaccc gctgccggaa ggccgaaagg tggtcggcac gacagcccgc
     9721 gcctgagaga tgaacgaccc tcttcaagac aagcctgacg acggcgacga cgcggaaaca
     9781 acttttccgg cgggcgtgcc cgaacaggaa gcggcaacgc tcgccttcac ggttgaggaa
     9841 ggggaagcgg gcgcgcgcct cgatgccttc ctcgccgcgc gtgtcgaagg agtcagccgc
     9901 tcgcggctca agcaatcaat cgaagacggc gacgtgctcg tcgcggggcg cacggccttg
     9961 aagggttctt acaaactgcg cgcgggcgag caggtcgaac tcgaaatgcc tgcgccgccc
    10021 gcttgcgatt tcatgcccga agagattccg ctcaaaatcg tctacgaaga cgcgtggctc
    10081 gtcgtcctcg ataagcccgc cggactcgtc gtacacccgg cagccggagt ctctacgggc
    10141 acgctcgcca atgcgctcgc cttccacttc cggcaactct ccgcgcacgc aggcgcgacg
    10201 cgaccgggca tcgtgcatcg tctcgaccgc gacacttccg gcctgatggt cgtcgccaaa
    10261 acggagcgcg cgcacgagca tctctccgat caatttcgcg cgcgggaagt cttcaagtct
    10321 tacgtcgcgc tcgtacacgg gcgcgtgcgg gaggagagcg ggcgcatcga agaaccgctg
    10381 gcgcgcgacc cgcgcacacg cacgcgcatg gccgtccgca gcggcgggcg tcccgcgctc
    10441 tcgctctatc gcatccggcg cgcttacgaa cgcttcacgc tgctcgacgt gcagatcaag
    10501 accgggcgca cccaccagat cagggtgcat ctcgcacacc tcaaacaccc ggtcgtcggc
    10561 gacaaggttt ataacggcgg gcgcgatacg acggtcgcag acgtgcgcgt gcgcgccgcc
    10621 atccgtgcga tggggcggca gtttctacac gccgaacaac tcggctttcg ccacccgcac
    10681 acgggcgaag acctgcgctt cgacgcgccc ctgcctacgg aactttcgca aatgctcgcc
    10741 gcgctcggtt gagccgcacg caactccccc tatcgccgcc gcgccgttcc accccacgct
    10801 cgcggcggcg cgtcagacgc ctacgcgccc ggcatgcgtt cgggcgggcg ggttgctata
    10861 ctgtggcgtt ctaaccaacg ccgcgccaat gggcaactga cgcttggccg cgcggcatcg
    10921 aaccctcgac gcttggacgt atcggcggct acagccttct gcccttccgt cagcccgatt
    10981 caaaaccatg agaactgcct tttattccct gctgttcgct ctcgcgctcg tgctctccgg
    11041 cgcgctcgcg ctcgacgctt ccgtctcggc tcagagttct aaccaacagc cgcgccccgc
    11101 cgcgccgtcg ccgcgcccga cgccccctcc gcaggatcag gacgacgtgc agggcggcac
    11161 agttttcacg cgcgaagtgc aactgccgat caccgtgatg gacaaaaagg atcagatggt
    11221 gtcgggactc aaggcgggcg acttcatcat ctacgaggac aagcagccgc agcagatcgc
    11281 gagtttcagg gacgagagcg caggcacgcc gatctacgtg gcggtcttga tggatatttc
    11341 atcctccgcc gccgccaagc tgcgtttcga gaaagagtcg gccaaggatt ttctctacac
    11401 ggtcgtgcgc gtgcgcaaag acaaggcggc cttcgccacc ttcgacgaag atgtcaacct
    11461 gctacaggat ttcacggaca agctcgacaa gctcgacacg gcggttgaca gcgtgaagaa
    11521 accgggcacg accaccgcgc tctacgatgc cgtctggcgc atgtgcgatg agaagatgcg
    11581 cggcgtcacg ggtcggcgcg tgatagtcgt catcaccgac ggcgaggaca cttcgagtcg
    11641 cgcgcggctc gaagaggcga ttgagatggc gcaacgcacc gagacgatca tcttcgccat
    11701 ttcgacaaag gcgggatttt cgagcgtcgt ccccggcgtc gaagcgggca cggtcaaaga
    11761 cgacgacgac aagcggctcg acaagctggc ggacgcaacc ggcggcaaag ccttcttcat
    11821 cggcgaccgc ctcgacctcg aacgcgcctt cacgaaagtg gcgaaggaac tgcgcgggca
    11881 gtacatcgtc acctacaggc cgacgaataa aaactacgac ggcaactatc gccgcatcga
    11941 agttaaactc gccgccgagc gcgacggcct cagagttcgc acgcgacgcg gctacacggc
    12001 acagccgatc agggcaaata atgaataatg aatgctgaag gcgaaacgat gaatgatgag
    12061 tgaaagataa aatcgcttct ctcttaattc atcgttccgc ctccggcatt cctgcaagaa
    12121 gggtttgaaa gtaatgtttg atcgcagcca gtttatgcgg gtgctcagtt ttattctcgc
    12181 gttctcggtc accttcggcg gcgcgcttgc gggcgcacgg gcgcaacagc agcagcagca
    12241 accggcgcaa caaccggctc cggcgcgcac gccgcccgcg cagcgagacg acaacacgac
    12301 gctcgacgac gacgagatcg agcgcgtcga taccagtttg acgaacgtga tcttcacggc
    12361 ggttgatcga gacaagcgtt ttctgtcgaa cctcaagcag gaagacatac gcgtgctcga
    12421 agacggcgtg ccgcaacaga ttttcacctt ccagcgaagc attgacctgc cgctcacgct
    12481 cgccatcgtc atcgacacga gcatctcgca ggagcgcacg ctgcccgaag agaaacgcgc
    12541 cgcgcgtgcc ttcgtggatg ccgtgatgcg cgccagcaag gacgaagtgg cggtgctctc
    12601 ttttaccggc gactcgacgc tcgaacaggg tttgaccggc aatccttcgc gcgtgcgcag
    12661 cgcgattgag cgcgtcgagt ttgtgccgcc ttcgggctac gtcgggcgcg gcgtcctcgt
    12721 ccgtggcggg acgcctccca tctccgggga taaccagtca accgccggtt cgaccgccat
    12781 ctgggatgct ctctacgcga cctgcgccga agtaatgacc gagtcttccg acaagacacg
    12841 ccgcgccatc atcctcctca gcgacggcga ggacacgagc agcactctga aactcgacga
    12901 ggccgtgcag gaggcgctca aaaccgagac gatgatcttc gccatcggca tcggcgacag
    12961 tttcaactat tcgggcgtgg acgaaggctc gctccgcaaa atcacggaac gcacgggcgg
    13021 tcgcgcttac ttcccgcgca gcgaagaaga cctccgcaac gccttcgagc agattcaacg
    13081 cgaactgcgc gagcaattcc tcgtcgccta ttccccgacc aacaagcggc gcgacggctc
    13141 ttatcgcaaa ctccagatcg aagttatcaa ccccgaactc cgcaaccgga acatcaagct
    13201 cagctaccgt cagggctact tcgccaagag cgacgccgct ccggcgaaaa ggcgcaggta
    13261 gagagacgaa gcgcgaaagt taagtcgaga taaaaccgaa agggaagacc ggcggaacat
    13321 tgcccgccgg tcttcccttt cggtttttca actttcgcgc ttcgcccgtc agccttctct
    13381 cttacctctc gccgcgctcg ttctccaccg tcgtttgcag ttcg
//
LOCUS       DASWNS010000428         7864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_145798, whole genome shotgun sequence.
ACCESSION   DASWNS010000428 DASWNS010000000
VERSION     DASWNS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145798"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VGB76_14890"
     CDS             complement(<1..243)
                     /locus_tag="VGB76_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100234.1"
                     /translation="MRWKLLIITSLSAALVGAGGDLALAYWRQGRSNPLSAPPALVAG
                     IVLIPLISVTLAGIFVYRHTARRRKLQATLTVLLSLI"
     gene            457..1377
                     /locus_tag="VGB76_14895"
     CDS             457..1377
                     /locus_tag="VGB76_14895"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY0100235.1"
                     /translation="MVTRNALLYLARQEGLKDFATRFRPFKKMTTRFVAGEDINEAVA
                     AIREINKLGASASFDHLNESVASRAETEEEVREYQRVLASIDETGIRSNVSIKLTQFG
                     LEIDPELAYRNAREVVADAARRGNFVRVDMEQSSVTQVTIDIFKRLRAEFDLNTVGIV
                     LQSYLRRTESDVADILKIPARIRICKGAYNEPPEVAFPDKKDVDANYIRVMRTLLSSG
                     VYHGIATHDPAMIEATINHAAREGIGKEAFEFQMLYGVRRDLQVQLARDGYNMRVYVP
                     YGKHWYPYFMRRLAERPANVWFVLKNMLKG"
     gene            1390..1896
                     /locus_tag="VGB76_14900"
     CDS             1390..1896
                     /locus_tag="VGB76_14900"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY0100236.1"
                     /translation="MSDFAWILWVILGVILIIAEIFTPGFVLLWFGIGAIAAALGAIV
                     GLGYPFQFLLFFFVSIALTAASRTIFTKYLMRETEQGGGYKSGAESLPGQVGTVVASS
                     QGALSEGAVKVYGSTWTAYPAEGEEPLEAGARVVVESVRGASIYVRRAPSEPDWRPSA
                     LPEGREES"
     gene            complement(1991..2929)
                     /locus_tag="VGB76_14905"
     CDS             complement(1991..2929)
                     /locus_tag="VGB76_14905"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY0100237.1"
                     /translation="MFVELYAQTDIGRVRQGNEDNFLVLDLASGGSWSGSDAATEPPE
                     KFRRFEVGREGLVLIVSDGMGGALAGDVASRMAVESVREMLTEEDAEHTTNDSGAKVP
                     LVDCLRNATGYANYAIHRKSLEDPRCSGMGATLTAAAIAGQAMDFVQVGDSRAYVIRG
                     DEIKLATKDQSLVQQLVDVGQITEEDAETHMFRNVILQALGAQSDLTPVLSRVRLQRG
                     DIMLLCSDGLSGKMRAEEMLQIVATSRGDLAAACDALIKEANKRGGEDNITVVLARFD
                     GEDLPAHASDPINVELAALEDDKTLPGTEDDTSPIP"
     gene            complement(3006..4148)
                     /locus_tag="VGB76_14910"
     CDS             complement(3006..4148)
                     /locus_tag="VGB76_14910"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEY0100238.1"
                     /translation="MGFQTTAIHAGQEPDAATGAVSVPIYQTSTYAQEGLGKHKGFEY
                     ARTQNPTRLALEQNIAALEGARFGYAFASGMAAIDTTLRLVKAGEHVVVSDNTYGGTY
                     RLFSRVLERYGIEFTYVDTSDASNVEAAMRDNTRMVFVETPTNPVMMVTDLQAVSDIA
                     HARGARVVCDNTFLSPYLQRPLEFGVDIVLHSTTKYLNGHSDSVGGVVVLNDEQDAEW
                     IKFIQNSVGAILSPFDSWLIMRGTKTLALRMEQHDRNGRAVAAFLEEHPKVEKLYYPG
                     SLSHRQHDLAKRQQRGFGGMVSFDVGSLASARTVLEGVRLCTLAESLGAVETLISHPA
                     TMTHASVLPETRARLGITDGLVRISVGLEDVDDIIGDIDQALGKIQ"
     gene            4470..5294
                     /gene="kdsB"
                     /locus_tag="VGB76_14915"
     CDS             4470..5294
                     /gene="kdsB"
                     /locus_tag="VGB76_14915"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF009905.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY0100239.1"
                     /translation="MKHTDSQPQISHAIAIIPARYHSTRLPGKPLLDICGRPMIVRVC
                     ERALAAKNVSRVLVATDDERIREAVLAAGYEAMLTSAEHASGSDRLAEAAAALDESFD
                     LIVNVQGDEPQISPRTIERAVAALQADADAACATTCEAVGDVSDVLSPDVVKVVSDER
                     GYAVYFSRSPIPYPRDAARRFGSLAAALEVEPALLTGFRKHTGLYVYRRAFLLEYARW
                     PQSALERAESLEQLRMLEHGARIRVVETTDASIGVDTPADLERVRAIFAAGKGDKT"
     gene            5291..5695
                     /locus_tag="VGB76_14920"
     CDS             5291..5695
                     /locus_tag="VGB76_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100240.1"
                     /translation="MTNDEIDRALDFLVKSQAAFDARQAAFEERQAAFEERQVAFEER
                     QSAFDVRLAQTNQQIAELGTQLRAYAETQSQFIQIVTESMTRLAEAQARTDQKIAESN
                     AWLTGQIADTNARLDRLAATVERNLKGDNGAS"
     gene            5753..6064
                     /locus_tag="VGB76_14925"
     CDS             5753..6064
                     /locus_tag="VGB76_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100241.1"
                     /translation="MSDWIGLAVILLIIAGGLFAASRLGAPPEKISPEEFDRRVREGA
                     ATRGAMFALQQWLHPKGAQAIEVQQDLRHGYYNKKKIPGDGDEAEVNAIRSSSSEHQG
                     E"
     gene            6064..7779
                     /locus_tag="VGB76_14930"
     CDS             6064..7779
                     /locus_tag="VGB76_14930"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY0100242.1"
                     /translation="MPKYIFVTGGVVSSLGKGLAASSIGCLLEARGLSVTLMKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTHARLTRANNWTTGRIYLSVIEKE
                     RRGDYLGKTIQVIPHITDEIKKAVRTVAEQDGADVVIVEIGGTVGDIESLPFLEAIRQ
                     MGNEESKDGQRGAIFVHLTLVPYIAAAGELKTKPTQHSVRELREIGIAADVLLCRSDR
                     NLSRDLRSKIALFCNVRERAVITAEDVDTIYDVPLAFHEQGLDELIVDSLGLQGRAAN
                     TDLAHWRALVATIREPAAGEVTIGIVGKYVELEDSYKSLREALTHGGVANNVRVNVRW
                     VESEKLMEENFEERLRDFDAILVPGGFGPRGVSGMIRAISYARRSRTPFFGICLGMQT
                     AVIEYARATCGLSNADSTEFDPATPYPVIFKLRDLVGVEELGGTMRLGSWQCRLAAES
                     LAREVYGGAPEIGERHRHRYEFNPEFRETLERAGLVFSGESPDGRFIEMVELPREAHP
                     WFLGCQFHPEYKSKPLDAHPLFTAFVRAALENRLRNETTVDESHEAEITATALAQRVG
                     VAGDD"
BASE COUNT         1558 a         2336 c         2471 g         1499 t
ORIGIN      
        1 gatgagcgag aggaggacgg tcagcgtagc ttgcagtttg cgccgccgcg ccgtatgcct
       61 gtagacgaag attcccgcga gcgtgacgga gatgagcggg atgaggacga ttccggcgac
      121 gagcgcgggc ggcgcgctca agggattcga acgtccctgt cgccagtaag cgagcgcgag
      181 gtcgccgccc gctccgacga gggcggctga cagtgaagtg atgatgagca acttccagcg
      241 caccgggcga aaatatcctt cgtcacgttc aaacggagag actgttaacg aggttgaagc
      301 agagagcagc ggcggagcaa cgtcaagcat atcataggag aagcaagtga caagttgaag
      361 agcgtaaatc gtgaatcgtc aatcgtaaat agaagagaac tgcttttaac tattcacgat
      421 tgacgattca cggctttctc tcgtcacttc aaaaaaatgg taactagaaa cgcactcttg
      481 tatctggcac ggcaggaagg cttgaaggac tttgccacgc gcttccgccc gtttaaaaag
      541 atgaccacgc gctttgtggc gggcgaagac atcaatgaag ccgtggccgc gattcgagag
      601 atcaacaagc tcggcgcgag tgcgtcgttc gatcatttga acgaatcggt cgccagccgc
      661 gccgagacgg aggaggaggt gcgcgagtat caacgcgtgc tcgcctcgat tgacgagacg
      721 ggcatccgct cgaacgtctc gatcaagctg acgcagttcg gcctcgaaat cgatccggag
      781 ttggcttacc ggaacgcgcg cgaagtggtg gcggatgcgg cgcggcgcgg caatttcgtc
      841 cgcgtggaca tggaacagtc gagcgtgacg caggtgacga tagatatttt caaacgcctc
      901 cgcgccgagt ttgacctgaa caccgtcggc atcgtcctgc aatcctacct gcgccgcacc
      961 gagagcgacg tggcagacat cttgaagatc cccgcgcgca tacgcatctg caaaggcgcg
     1021 tacaacgaac cgcccgaagt ggctttcccc gacaagaagg atgttgacgc caactacatt
     1081 cgcgtgatgc ggacgctcct gtcgagcggc gtctatcacg gcatcgcgac gcacgatccg
     1141 gccatgatcg aggcgaccat caatcacgcc gcgcgcgagg gaatcgggaa agaggctttc
     1201 gagtttcaga tgctctacgg cgtcaggcgc gacctgcaag tgcaacttgc gcgcgacggg
     1261 tataacatgc gcgtctatgt gccttacggc aagcactggt atccttactt catgcggcgg
     1321 ctggcggagc gtccggccaa cgtctggttt gtcctgaaga atatgttgaa gggttgaagc
     1381 gggagagaga tgagcgactt cgcttggata ctgtgggtga tcctcggcgt gatcctgatc
     1441 atcgcggaaa ttttcacgcc gggcttcgtc ctgctctggt tcggcatcgg cgcgattgcc
     1501 gccgcactcg gcgcgattgt cggcttgggc tacccgtttc aattcctgct cttcttcttc
     1561 gtctcgatag ctttgaccgc cgcttcgcgg acgatcttca ccaagtatct gatgcgcgaa
     1621 acggagcagg gcgggggcta taagtcgggc gcggaatcgc tgccgggaca ggtgggcacg
     1681 gtcgtcgcat cgagtcaggg cgcgctctcg gaaggcgcgg tgaaagttta cggctcgacg
     1741 tggaccgcct acccggcgga aggcgaagag cctctggaag cgggcgcacg cgtcgtcgtc
     1801 gagagcgtgc gcggcgcatc gatctacgtc cgccgcgccc cctccgaacc cgactggcga
     1861 ccgagcgcgc tgcccgaagg aagggaagag agctaaaggg aaaaggggaa ggggtaaggg
     1921 tgaaagcaag cggcgcacga tagacgcgcc tactcgtttt tacctttacc ccttcccctt
     1981 ttcctctgtc ttaaggtatg ggcgaagtgt cgtcttcggt gccggggagt gtcttgtcgt
     2041 cttcgagtgc ggccaactct acgttgatcg ggtcggaggc gtgggcgggc aggtcttcgc
     2101 cgtcgaagcg tgcgaggacg actgtgatgt tgtcttcgcc gccgcgcttg ttggcttcct
     2161 tgatgagcgc gtcgcaggcg gcggcgaggt cgccacggct cgttgcgacg atctggagca
     2221 tctcttcggc gcgcatcttg ccggagaggc catcgctaca gagcagcatg atgtcgccgc
     2281 gttgcaggcg gacgcgtgag agcacgggcg tcaggtcgct ctgcgcgccg agagcttgga
     2341 ggatgacgtt gcgaaacatg tgcgtctcgg cgtcttcttc cgtgatctgc ccgacatcca
     2401 cgagttgctg tacgagcgac tgatctttgg tagcgagttt gatctcatcg ccgcgaatga
     2461 cgtaggcgcg gctgtcgcca acctgtacga agtccatcgc ctgcccggcg atggcggcgg
     2521 ctgtcagcgt tgcgcccatg ccgctgcaac gcgggtcttc gaggctcttg cgatgaatgg
     2581 cgtagttggc gtagccggtg gcgttgcgca ggcaatccac gagcggcact ttcgctcccg
     2641 aatcgttcgt cgtgtgctcg gcgtcctctt cggtgagcat ctcgcgcacg ctctcgacgg
     2701 ccatccggct cgccacgtcg cccgcgagcg cgccgcccat gccgtccgag acgatgagca
     2761 ccagcccctc tctgcccact tcaaagcggc gaaatttttc gggcggctcc gtggcggcgt
     2821 ccgagccgga ccaactcccg cccgatgcga gatcgagcac gaggaagtta tcttcgttgc
     2881 cctgccggac gcggccaatg tcggtttgtg cgtatagctc gacgaacata ggttaagtga
     2941 tgagtgacag gtgacgaact ggaagagcga cggattttaa cttgtcactc atcacttgtc
     3001 actggtcact gtattttacc gagtgcttga tcaatatccc cgatgatgtc gtccacgtct
     3061 tcgaggccga cgctgatgcg cacgaggccg tcggtgatgc cgaggcgtgc gcgcgtctcc
     3121 gggagcacag aggcgtgcgt catcgtggcc ggatgcgaga tcagagtttc caccgcgccg
     3181 aggctttcgg caagcgtgca gaggcgaacg ccttcgagca ccgtgcgcgc gcttgcgagc
     3241 gagccgacat cgaaggaaac catgccgccg aaaccgcgct gctgccgttt cgccagatcg
     3301 tgctggcgat gcgagagcga gccgggataa tagagcttct caaccttcgg gtgctcttcg
     3361 agaaaggcgg cgacggcgcg cccgttgcgg tcgtgctgtt ccatcctgag cgcgagcgtc
     3421 ttggtgccgc gcatgatgag ccacgaatcg aagggcgaca ggatcgcgcc cacgctgttt
     3481 tgaataaact tgatccactc ggcgtcctgt tcgtcgttca agaccacgac gccgccgacg
     3541 ctgtccgaat ggccgttcag atatttcgtc gtcgagtgca ggacgatgtc cacgccaaat
     3601 tccagcgggc gttgcaggta gggcgagaga aaagtgttgt cgcacaccac acgcgcgccg
     3661 cgtgcgtgcg cgatgtccga gacggcctgc aagtccgtca ccatcatcac ggggttggtc
     3721 ggcgtctcga cgaagaccat gcgcgtgttg tcgcgcatcg cggcttcgac gttcgaagcg
     3781 tccgacgtat cgacataagt gaactcgatg ccgtatcgct caagcacgcg cgagaagagg
     3841 cggtacgtgc cgccgtaggt gttgtcggag acgacgacgt gctcgcccgc tttgacgagg
     3901 cgcagcgtcg tatcaatggc cgccatgccc gacgcgaaag cgtagccgaa acgcgcgcct
     3961 tcgagcgcgg cgatgttctg ttcgagggcg aggcgcgtgg ggttctgcgt gcgcgcatac
     4021 tcgaagccct tgtgcttgcc gagtccttcc tgcgcgtaag tcgaagtttg atagatgggc
     4081 acggagactg cgcccgtcgc cgcgtccggt tcctgtcccg cgtggatggc tgtcgtttga
     4141 aatcccatag cttagtagat cgtgaatggc aaagggtaaa actttgataa ccgtcgaaac
     4201 tcaggcgaac attcgcgcga ggtcactttt cttccgaagt cagagctgaa aaactttgcg
     4261 catcgtagcc cgccccaagt gcggtgtcaa acggctcaaa gacgggagga ttggggagag
     4321 gcagaagggg aagcgggaaa gggattcgtg cgaatacgcg gcgactgcga acctttgacg
     4381 cctttcccct ttggccgttc ccccttgccg ctgcttttca tttgcttttc attgacactc
     4441 gcgtgcgcga caggctatct tgcaatcccg tgaagcacac ggactcccaa cctcaaatct
     4501 cccacgccat cgccatcatc cccgcacgct accactcgac cagacttccg ggtaaacctc
     4561 tgctcgacat ctgtggacga ccgatgatcg tgcgcgtctg cgaacgcgcg ctcgcggcga
     4621 agaacgtttc gcgcgtgctg gttgcgaccg acgacgagcg catccgcgag gccgtgctcg
     4681 ccgcaggcta cgaggcgatg ttgacgagcg cggagcacgc gagcggaagc gaccgtctgg
     4741 ccgaagccgc cgccgcgctc gatgaaagtt tcgacctcat cgtcaacgtg cagggcgacg
     4801 agccgcagat ttcgccgcgc accatcgaac gcgccgtcgc cgccttgcag gcggacgccg
     4861 acgccgcctg tgcgacgacg tgcgaggccg tcggggatgt gagcgacgtg ttaagtcctg
     4921 atgtggtcaa agtcgtgtcg gacgagcgcg gttatgccgt ctatttttcg cgttcgccga
     4981 ttccataccc gcgcgatgcc gcacgccgtt tcggttcgct cgccgccgcg cttgaagtcg
     5041 agcctgcgct gctcacgggg tttcgcaagc acacggggct ttatgtttac cgtcgcgcct
     5101 ttttgttaga atacgcccgt tggcctcagt ccgcgctcga acgcgccgaa tcgctcgaac
     5161 aactgcgcat gctcgaacac ggcgcacgga ttcgcgtggt cgagacgacg gacgcgtcaa
     5221 tcggcgtgga cacgcccgca gacctcgaac gcgtgcgcgc aatatttgcc gccgggaaag
     5281 gtgataagac atgacaaacg atgagataga ccgtgcgctt gatttcctcg tgaaaagtca
     5341 ggccgctttc gacgcacggc aagccgcctt tgaagagagg caagccgcct ttgaagagag
     5401 gcaagtcgcc ttcgaagaga ggcaatctgc tttcgatgtg cggctggcgc aaactaatca
     5461 acagatcgca gaactcggca cacagttaag agcctacgcg gagacgcaaa gccagtttat
     5521 tcagatcgtg acggaaagca tgacgcggct ggcggaggct caggcgcgca ccgaccagaa
     5581 aatcgcggag tcgaacgcct ggttaaccgg acagattgcg gatacgaacg cgcgtctgga
     5641 tcgtctggcg gcgaccgtcg aacgcaacct caagggcgac aacggggctt cctgatctgt
     5701 tttcgttttt acttgaaatt gtcggtcatc ggctcactcg cctctgtatc ttatgagcga
     5761 ctggatcggg ttagcggtta ttctcttgat catcgcgggc ggcctcttcg cggcgtcgag
     5821 gctcggtgcg ccgcccgaaa agatttcgcc ggaagagttc gaccggcgcg tcagggaagg
     5881 cgcggcgacg cgcggggcga tgttcgcctt gcagcaatgg ctgcacccga aaggggcgca
     5941 ggccatcgag gtgcagcagg atttgcgaca cggctactac aataagaaaa agattccggg
     6001 cgacggcgac gaggccgaag tcaatgcaat aagatcaagt tcgtccgaac atcaaggaga
     6061 gtgatgccta aatatatttt cgtcacgggc ggcgtggttt cgagtctggg caaggggctg
     6121 gcggcatcgt ccatcgggtg cctgctcgaa gcgcgcggcc tgtccgtcac gctgatgaaa
     6181 ctcgacccgt acatcaacgt cgacccgggc acaatgtcgc ctttccagca cggcgaagtc
     6241 ttcgtcacag acgacggcgc ggagacagac ctcgacctcg gccactacga acgcttcacg
     6301 cacgcacgcc tgacgcgcgc caacaactgg acaaccgggc gcatctatct ctccgttatc
     6361 gaaaaagagc ggcgcggcga ttatctgggc aagaccatac aggtcatccc gcacatcacg
     6421 gacgagatca aaaaagccgt gcgcacggtc gccgaacagg acggcgcgga tgtcgtcatc
     6481 gtcgagatcg gcggcacggt gggcgacatc gaatcgctcc cgtttctcga agccatccgc
     6541 cagatgggca acgaagagtc gaaggacggc cagcgcggcg cgatcttcgt acacctcacg
     6601 ctcgtcccct acatcgccgc cgcaggcgaa ctcaaaacca agcccacgca acactccgtc
     6661 cgcgaactgc gcgagatcgg catcgccgca gacgttttgc tctgccgctc cgaccgcaat
     6721 ctctcgcgcg acctgcgctc caagatcgcg cttttttgta acgtcaggga gcgcgccgtc
     6781 atcaccgccg aagacgtgga cacgatctac gacgtgccgc tcgccttcca cgaacaggga
     6841 ctcgacgagt tgatcgtaga ttcgctcggt ttgcaggggc gcgccgccaa cacagacctc
     6901 gcgcactggc gcgcgctcgt cgccacgatc cgcgagcctg cggcgggcga agttaccatc
     6961 ggcatcgtcg gcaagtatgt cgagttggag gattcctaca agtctctgcg cgaggctctg
     7021 acgcacggcg gcgtcgccaa caacgtgcgc gtgaacgtgc gctgggtgga gtcggagaag
     7081 ttgatggagg agaacttcga ggagcgtctg cgcgacttcg acgccatcct cgtgcccggc
     7141 ggcttcggac cacgcggcgt ctccggcatg atccgcgcca tctcttacgc gcgccgcagc
     7201 cgcacgccct ttttcggcat ctgcctcggc atgcaaaccg ccgtcatcga atacgcgcgt
     7261 gcgacgtgcg ggctgtccaa cgccgattcg accgagtttg atccggcgac gccctatccg
     7321 gtcatcttca aattgcgcga cctcgtcggc gtagaggagt tgggcggaac gatgcggctc
     7381 ggttcgtggc aatgccgcct cgccgctgaa agtctcgcgc gcgaagttta tggcggcgcg
     7441 ccggagatcg gtgagcggca ccggcaccgc tacgaattca atcccgaatt ccgtgagact
     7501 ttggagcgcg ccggactcgt cttcagcggc gaatcgcccg acgggcgttt catcgagatg
     7561 gttgaactcc cgcgcgaggc tcacccgtgg tttctcggct gccagtttca ccccgaatac
     7621 aagtcgaagc cgctcgatgc acacccgctc ttcacagcct tcgtccgcgc cgcgctggaa
     7681 aatcgcctgc gcaacgaaac gaccgtggac gaatctcacg aagcagagat aaccgccacc
     7741 gcgctcgcgc aacgcgtcgg cgtcgccgga gacgactgaa agacagtgac gagtgacagg
     7801 tgacaagtga caagttgaaa aaccgtcaat cgtcaatcgt gaatagagga agagcggtct
     7861 tcct
//
LOCUS       DASWNS010000429         8717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_484999, whole genome shotgun sequence.
ACCESSION   DASWNS010000429 DASWNS010000000
VERSION     DASWNS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484999"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VGB76_14935"
     CDS             complement(<1..436)
                     /locus_tag="VGB76_14935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0100243.1"
                     /translation="MTEPKPTQEGLRTSEIRYRRLFETARDGILILSAVTLKITDVNP
                     FMTELLGYSSDEFLGKELWEIGFFSDKEASQAAFRELQETGYLRYEDLPLQTLLGESR
                     EVEFISNVYEEDGRQVIQCNIRDIGERKRSQAECTRLLECAMA"
     gene            complement(707..1414)
                     /locus_tag="VGB76_14940"
     CDS             complement(707..1414)
                     /locus_tag="VGB76_14940"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0100244.1"
                     /translation="MSQTAFEPTGNRLLARLPGEEMERLLPHLEHVSFALGEVIYESG
                     GRQSYIYFPTTAIISLLYMMENGSSAEMGVAGNDGLVGIALFMGGDTVPNRAVVQSAG
                     DALRMKTKVLQEQFAHGGMFQRLLLRYTQALMTQMSQTAVCNRLHSVEQQLCRWLLLS
                     RDRLDSDELVMTQELIANMLGVRREGVTQAAGRLQETGLISYVRGRIQILDRSGLEAT
                     VCECYRVVKDEYHRLLG"
     gene            1618..2346
                     /locus_tag="VGB76_14945"
     CDS             1618..2346
                     /locus_tag="VGB76_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0100245.1"
                     /translation="MSNNSRLPPIQNQLLAALPAKDYKRLLSHLESVPLPFMEVLYES
                     GDAIEHVYFPNDGLISLLVVMKDQTLREIGVIGSEGMLGVAVAMGMKATANRALIQMP
                     GSAVRMKADTLRDELKLCGALQNVLHAYAHSLFTQVSQSAACTSAHALDKRLSRWLVM
                     THDHAPGDVFEMKHEFLAMMLGVTRSVVTRAAGLLQDEKMIRYTRGQMTILDRSRLEA
                     TACECYAVVKAEYARNNAHNTRGG"
     gene            2689..3309
                     /locus_tag="VGB76_14950"
     CDS             2689..3309
                     /locus_tag="VGB76_14950"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0100246.1"
                     /translation="MGATGFVGRAILEELLADGSVAAVHALGRRDPGIIHSKLTSHVV
                     DFRALPQLPPLDEVYLALGTTIKAAGSQAAFHALDFDANLAVARAALAAGARRAGLVS
                     AMEADTKSRIFYNRVKGELEEALARLSFDGLVLARPSLLTGDREALGQPKRQGERVAT
                     VVSKLLGPLIPANYRPIAATDVARALLARVPAAQGRVVLLSGEMQG"
     gene            complement(3651..5762)
                     /locus_tag="VGB76_14955"
     CDS             complement(3651..5762)
                     /locus_tag="VGB76_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0100247.1"
                     /translation="MKRTTCLLLLTALLLLVNAEGGSPSAAQRRRGRPDRAQEGSSIT
                     VKTKHPNRKNPQDYYSCPNCDLRGVDLSGKYMESANLSGADLTGAKLRGTNLDGASLV
                     GTILDGADLEGAVLNPSVKGVADLARASLRGASLKGAQMLGADLQFAHMEGADFTGAN
                     LSRAAFGPRIRAGVFNGRKTTFRRARLRRDFKTDEASMDIADVQWEPSPGAGAGEALV
                     CGWADLSGLTSRIYVSPGGSDSETCGTSNEQSCKTIAKGIERCSGQGCGVLVEWAEYN
                     IAATLRLRDGVNLYGGCLPASAARPELFSVIYAAPGGQPVVSADAINTNATILQGFQL
                     SGSASGGTNGESSVALLVTNSSKLSVLNSEIVANTGSKGADGQGNSSGMSGNNASKRT
                     RGANSFCSNTDGGDGAGEYAVYVINYAFSFQCVGSCPNGCTGSTGLSGSTGYFARGGR
                     GPGNPNCHECPASKGLTGGAGEGGRNAGCGVKGNVSTDTAGSFRGNAWTGSVSGSGTR
                     GGDGGGGGGGGSGGYKAGSCFYVKTEFYGNSGGGGGAGGCGALGGGGGRQGGASFAAV
                     LVNSTVTIKDSRLIGGLSGAGGMGGSASSGGKGGKGADGDTNQGGGYGGKGGEGGVGG
                     AGAGGAGGNGGPAVCVALVGNSALPGAPAHYYVGASGATGAPGRGGLAGVCNGPDGDQ
                     GSQGLVAEAHQFSAGARPRTR"
     gene            complement(5804..>8717)
                     /locus_tag="VGB76_14960"
     CDS             complement(5804..>8717)
                     /locus_tag="VGB76_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100248.1"
                     /translation="HAAMDYLSDAKTLIFHHPDLINNDPEVTHIINEYMDEQKIDAMF
                     QAVAMQMRQMGQPTENSGWARLEPFTPEPNPETHIPDETFYFAQPTLTIIEAAGSVAT
                     AMMKATKNDERLEGKKWEQQTGQSVENENGPDQLTLAARIEAESGKDWRVSLTNTREI
                     NGFKVEIEQYPGARALKLTLTNFYIRYLGAYIRFYDANGNAMSLPDWKSDNFVANVMT
                     EVIDIQYDDLRFIGHIMPINNIMAIPIRLDPGKLVVNIMFPPNAVSASIYGAGLGTGE
                     NLWPKTTIVGGVLTGLINLGVPAFMLAFAVAAQSYWPLYKIIDDLSKNDKFVGAVIGG
                     GMSLFGLKFAHTGVARKEMDWQTFSTVAKLLFDVSAAKALTWVTAQTVAGQVTGQIPF
                     AGWILVALNVAIGIAQLAQTIVAVATSPWNIQNQLATSITTNVTLYPDPRHHAFPQPP
                     AGSQASYSVKMIYKDQKRATISQTHDVPSGSTALTLPAAFPNNTLGGQVKFEVSFFID
                     EWLAAKATTGWMYNDEWHVSQVNLYLIQYPVPLTDKSVYVHTALLAYQNGAYVWHQTP
                     TAPTATIANRNTSSVGNAISEWMGLTLSQRYGMIGFAWKAAGMGINSCVSGQGTGQLF
                     AMQNINIPGMEMSALKFPNCGFDGPTQIVYDPYPPKFLMQNDQWVIVNGKPVVDPNST
                     LLGEYYIDPRKADVSLSEGGGYHLRRVTLDNSTPFDTRADQLSWGCFSYFPNSIALHP
                     SSNVIGVNTQYKKIQVAKLVSEGLPDKDMPVAKVYAGEALKIDRKGLLFRPLGVTCAY
                     DGTIFILEDTKSAGGLLALNFIVARIQAFDLYGNPVNRFFDSEGQPSPFLYLSEAPDF
                     NYLDIVAVGDEKMTYLYVLFYTGNGGSPSDYHMSIYQYGTKAPVNNPLVTTDNVAAAR
                     IAVDMWHTMYALNYDMTTNGQGNNPAGPHNSTTGPAGRTVPSVSEWLPPVLKDDEA"
BASE COUNT         1683 a         2605 c         2627 g         1802 t
ORIGIN      
        1 ccgccatcgc acactccaac agccgcgtgc attccgcctg tgaacgcttg cgttcgccga
       61 tgtcgcggat gttgcattga atgacttgcc ggccgtcttc ctcataaaca ttgctgataa
      121 actcaacctc acggctctct cccagcaggg tttgcagcgg caaatcttcg taacggagat
      181 agcctgtctc ctgcaactct cggaaggcgg cctggctcgc ttccttatcg ctgaagaaac
      241 cgatttccca aagctccttg cccagaaatt cgtcgcttga gtagcccaac aattccgtca
      301 taaacggatt cacgtcggtg atcttcagtg tgacggcgct cagaataagt atcccgtccc
      361 gcgcggtctc aaagagccgc cggtagcgga tttctgaagt ccgcaaccct tcctgcgtcg
      421 gctttggttc agtcatgttg ttggcctcat cggaaacaga gacgagtggc tggttttcaa
      481 ccagcctgac tcgctttatg gatagcaggt ggaactgcct cgtttggtca ggtcggaaaa
      541 gacgccagac ggcatttgtg aggggaaaga gattggctcg ctccggttgt cgtgaactga
      601 agtcccggcg agtattaacc ctattatgag taacacgaga ggggcgaggt cgtccgtgcg
      661 ctaacacact tataaaggga cgtgaggaaa ggataagaag agactttcag ccgagcagac
      721 ggtggtactc gtctttgacc acccggtaac actcgcagac cgtcgcttcc aagccgctcc
      781 ggtctagtat ctggatgcga ccgcgcacgt aactgattag tccggtttcc tgcaaacgac
      841 ccgccgcttg tgtcacgcct tctctgcgca cccccagcat gttggcgatt agttcctgcg
      901 tcatcaccaa ctcatccgag tctaagcggt cgcggctcag cagcaaccag cgacagagtt
      961 gctgctctac cgagtggaga cgattgcaga cggccgtctg tgacatctgt gtcatcaacg
     1021 cctgcgtgta gcgcaggagc aggcgttgaa acatgccccc atgcgcgaac tgctcttgca
     1081 gcactttcgt cttcatcctg agagcatccc cggcgctctg cacgacggcg cgattaggca
     1141 cggtatcgcc ccccatgaaa agggcaatac cgacaagtcc gtcattgccc gctacaccca
     1201 tttcggctga cgagccgttc tccatcatgt agagcagaga gatgatggcg gtggtcggga
     1261 aatagatgta actttgccgc cctcccgatt catagatgac ctcgccgagc gcaaaggaga
     1321 cgtgctctaa gtgtggcagg agacgctcca tttcctctcc gggaaggcgg gcgagcagcc
     1381 gattgcctgt cggttcaaat gcagtctgag acatggtgtg tttcctttca cggacagctt
     1441 tggcgattgt acattctata tgtgtgctag agcacggacg ggttgaatca gatcgagtat
     1501 atttgcgata cctgttcttt taggattcat ctctcgacat caggacttga ctagcgaagc
     1561 gtctccttgt gccgtctgct ggtttcacca cgggctacaa tctaaggagc gctccaagtg
     1621 tcaaataact ctcgactacc gccaatccag aaccaactgc tggccgccct gcccgctaaa
     1681 gattacaagc gtctgctttc ccaccttgaa tccgtccctc tgccgttcat ggaagtcctc
     1741 tatgagagcg gagacgcaat cgaacatgtc tacttcccca acgacggact catctcgttg
     1801 ttagtcgtca tgaaagatca gacgctcaga gaaatcggcg tcatcggtag cgaagggatg
     1861 ctcggcgtcg cggtggcgat ggggatgaag gcgaccgcga accgcgcgct cattcagatg
     1921 ccgggcagcg ccgtgcggat gaaggctgac acgctgcggg acgaactcaa actctgcgga
     1981 gcattgcaga acgtgctgca cgcttacgct cactcgctct ttacacaggt ctctcaatcg
     2041 gcggcctgca cgagcgcgca cgcgttggat aaacgcctct cacgctggct ggtgatgacg
     2101 catgatcatg cgccgggcga tgtgtttgag atgaagcacg aatttctggc gatgatgctc
     2161 ggtgtgaccc gttccgtggt aacgcgggct gccgggctac ttcaagacga aaagatgatc
     2221 cgctatacgc gtggacaaat gaccatcctc gaccggagca ggctggaggc gacggcctgt
     2281 gagtgctacg cggtggtcaa agcagaatac gcgcgtaaca acgcgcacaa cacacggggg
     2341 ggttgagatg gaaaagatgc gtgccaccgt tttccatggc gtgaacgata ttcgcgttgg
     2401 cagggagtaa ataaaagggc ggtgacttat ggacggcagc gggcgtaaaa acgtaaggcc
     2461 gggactcacg gtcgatatcg tcatgaagaa ggatcaatga accgagaggc tgacgcgcgg
     2521 cgtcgtcaga gacgtgctga caaaatcccc gttccacccg cacggtatca aggtcaggtt
     2581 agagaccggt gaagtgggca gagtcaaaga ggtcgttaac gagggaatcg ggtgacggag
     2641 atgcctcctc tcagtccgaa cgggtcacac cctagggtcg tagcgttggt gggggctacg
     2701 ggctttgtcg ggcgggccat cctggaagag ttgcttgccg atggctccgt cgccgccgtc
     2761 cacgcactag gtagacgcga tcccggcatc attcactcca agctcacttc acacgtggtg
     2821 gacttccggg cgctaccgca gctcccaccg ctcgacgagg tctatctggc gctcggcaca
     2881 accatcaagg cggctggcag ccaggcagcc ttccacgcct tggacttcga cgccaatcta
     2941 gccgtcgccc gcgcggcact cgccgcgggc gcccggagag ctggccttgt tagtgcgatg
     3001 gaggcagaca cgaagtcacg catcttttac aaccgcgtga agggtgaact ggaggaggcg
     3061 ctggcgcggc tctctttcga cgggctggtc ctcgcccgcc cgtctttgct gacaggagac
     3121 cgtgaggcgc ttggccagcc aaagcgccaa ggcgagaggg tggcgacggt agtgagcaaa
     3181 ctcctcggac cgctcattcc ggcgaactac cggcctatcg ccgcaaccga tgtcgcccgg
     3241 gcgctgctgg cgcgcgtgcc tgcggctcaa gggcgcgtgg tgctgctctc cggcgagatg
     3301 cagggctgag cgacagccga gagaacagtc caccgttctt tacgaccgag taactggatg
     3361 gcagaagaac tgagcgcaag agaccttaga cgatagaatc agtatgcgcc tgtgctcgtg
     3421 tctctacgcg atgtgcaaga ccgtatatcg aaggcaagga ttatcaaagg gcgttcgatt
     3481 cagctctacg acaagcagga aagtcaattc attaaaattg tgcgttatcc ctacagtgca
     3541 gtaaagcctt atagccacga ctccgagata agggctaaat agccttccgt atatgaatag
     3601 ctacttctcc tccaaataaa gtctttacat tatgccacta aaggacatct tcatcgcgtg
     3661 cgtggacggg ctcccgcgct gaactggtgc gcctctgcga cgagcccctg actgccctgg
     3721 tcgccgtccg gcccgttgca gacgcctgct aggccgcctc ttccgggcgc gcctgtcgct
     3781 ccggacgctc cgacgtagta atgcgcgggc gcgcccggca aagcggagtt gccgacgaga
     3841 gcgacgcaga cggcaggccc gccgttgccg cccgcgcctc cggctccggc tccgcccacg
     3901 ccgccttcac cgcccttgcc gccataacct ccgccctggt tcgtgtcgcc gtcggcaccc
     3961 ttgccgccct tgccgcccga agaagcactg ccgcccatcc cgcccgcccc gctcagcccg
     4021 ccgatcaggc gagagtcttt gatcgtcacc gtcgagttga cgaggacggc ggcgaatgac
     4081 gcccctccct gacgacctcc gccgccgccc aacgcgccgc agccgccagc gccgcctccg
     4141 ccgccgctgt tgccgtagaa ctccgtcttg acatagaagc aggaccctgc cttgtagccg
     4201 ccagagccgc ctccgccgcc tccgccgccg tcgccgcctc tcgtccctga accgctgacg
     4261 cttcccgtcc aggcgtttcc gcgaaagctg cccgccgtat cggtcgagac gttccccttc
     4321 acgccgcatc ccgcattgcg gccaccctca ccagcgccgc cagtgagtcc tttactggcg
     4381 gggcactcat gacagttggg gttgccgggg ccgcggccgc ctcgggcgaa gtagccggtc
     4441 gagcctgaaa gacccgtgga gccggtgcag ccattcgggc acgagccgac gcattggaaa
     4501 ctaaaagcgt aattgatcac gtagaccgca tactcgcctg caccgtcgcc tccatcggtg
     4561 tttgagcaga atgaattggc cccgcgcgtg cgcttgctgg cattgttgcc gctcatgccg
     4621 ctgctgttgc cctgcccgtc agcgcccttg ctgcctgtgt tcgccacaat ctccgagttg
     4681 agcacggaga gtttggaact gttcgtcacc agcagcgcga cgctggactc gccgttcgtc
     4741 ccgcccgacg cgctgccgct cagttggaac ccttgcagga tggtcgcgtt ggtgtttatc
     4801 gcgtcggcgc tgacgactgg ctgtcctccg ggcgctgcgt aaatgacgga aaataattcc
     4861 ggcctggccg cgctcgccgg aaggcatccg ccgtagaggt tgacgccgtc gcgcaggcgc
     4921 aatgtcgccg cgatgttgta ctctgcccac tcgacgagca cgccgcagcc ctggccggag
     4981 cagcgctcga ttcccttcgc gatggtctta caggactgct cgttgcttgt cccacatgtt
     5041 tcgctgtctg agccgccggg cgagacgtag attctcgaag tcagcccgga gaggtctgcc
     5101 cagccgcata cgagggcttc gcccgcgccc gcgccggggg aaggctccca ctgcacgtcc
     5161 gcgatgtcca tcgaagcctc gtccgttttg aagtcgcggc gcaggcgggc gcggcggaag
     5221 gtggtcttgc ggccattgaa gacgcccgcc ctgatgcgcg ggccgaaagc cgcccggctc
     5281 aggttcgcgc cggtgaagtc cgcgccctcc atgtgtgcga actgcaagtc cgcgccgagc
     5341 atctgcgcgc ccttgagcga agcgcccctg aggctcgccc gcgcgaggtc tgcgaccccc
     5401 ttcacggaag gattaagcac cgcgccttcc aagtccgccc cgtccagaat cgtcccgacg
     5461 agcgaggccc cgtcgaggtt tgtcccgcga agcttggccc cggtcaggtc ggccccggac
     5521 aggttcgcgc tctccatgta cttgcccgag aggtcaaccc ccctcaaatc gcagttcggg
     5581 cacgagtaat agtcctgcgg gttcttgcgg ttcgggtgct tggtcttgac ggtgatgcta
     5641 gacccctcct gcgccctgtc gggccgacct cttcttctct gcgccgcgga gggcgacccg
     5701 ccctccgcgt tgacgagaag caggagggca gtgaggagca gcagacaagt tgttcgtttc
     5761 atgagtttct cccggaaggc ttaagcctca tctctcttga ccgctaagcc tcgtcgtctt
     5821 tgagcacagg gggcagccac tcgctgacgg aagggacggt gcggcccgcc gggcccgtcg
     5881 tagagttatg tgggccagcg ggattattac cctgcccgtt cgtcgtcatg tcgtaattga
     5941 gcgcatacat cgtgtgccac atgtctacgg cgatgcgcgc cgccgctacg ttgtccgtcg
     6001 tcacgagcgg attattgacg ggagccttgg tgccgtactg gtagatggac atgtgatagt
     6061 cagagggcga accaccattg cccgtgtaga agaggacgta aagataagtc atcttctcat
     6121 cacccacggc cacgatgtcc aggtagttga agtccggcgc ttccgaaaga tagagaaacg
     6181 gcgaaggctg gccttcactg tcgaagaagc gattgacagg gttgccgtag aggtcgaacg
     6241 cctgtatgcg cgcgacgatg aaattcaagg ccaggagtcc acccgccgac ttggtatctt
     6301 cgaggatgaa gatagtgccg tcgtaggcac acgtcacgcc cagaggacga aagagcagcc
     6361 ctttgcgatc tatcttgagc gcctcaccgg catacacttt cgcgaccggc atatccttat
     6421 cgggaagccc ctcgctgacg agtttggcga cctgaatctt tttgtactgc gtgttgacgc
     6481 cgatgacgtt gcttgaagga tgcaacgcaa tcgagttcgg gaaataggag aagcatcccc
     6541 aactcaattg atccgcgcgt gtgtcgaagg gagtgctgtt atcgagcgtg acacggcgca
     6601 ggtgatagcc gccgccctcg ctgaggctca catcggcttt acgcgggtct atgtaatatt
     6661 caccgagcag agtgctatta gggtcaacga ctggcttgcc gttgacgatc acccactggt
     6721 cgttctgcat gaggaacttc ggcgggtacg ggtcgtagac gatctgcgtg ggcccatcga
     6781 aaccgcagtt cggaaacttg agcgcggaca tctccattcc cggaatgttg atgttctgca
     6841 tggcgaagag ttggcccgtg ccctggccgc taacacacga gttgatgccc atcccggcgg
     6901 ccttccaggc aaagccgatc atgccgtagc gctggctgag cgtgagcccc atccattccg
     6961 aaatggcgtt gccgacgctg ctcgtgttgc ggttggctat cgtcgccgtc ggcgcggtcg
     7021 gagtctggtg ccagacgtaa gcgccgttct ggtaggcgag cagcgccgtg tgcacgtaga
     7081 cggatttgtc cgtcaaaggc actggatact ggataagata aagattgacc tgcgaaacgt
     7141 gccactcatc gttgtacatc cagccggtgg tggccttcgc ggcgagccat tcgtctatga
     7201 agaagctgac ctcgaacttg acctgcccgc cgagcgtgtt gttcgggaag gcggccggaa
     7261 gcgtcagagc cgtcgagccg gagggcacat catgcgtctg cgagatggtg gcccgctttt
     7321 ggtctttgta aatcatcttc acgctgtagg aagcctgcga cccggccgga ggctgcggga
     7381 aggcatggtg gcgtgggtcc gggtagagcg tgacgttggt ggtgatggag gtcgcgagct
     7441 ggttttgaat gttccagggc gaggtcgcca cggcgacgat ggtctgggca agctgagcga
     7501 tgccaatcgc gacgttgagg gcgacaagaa tccatccggc gaagggaatc tggccggtga
     7561 cttgcccagc cacggtttgc gccgtgaccc aggtcagtgc tttggctgcg gagacgtcga
     7621 agagcagctt cgctaccgtc gagaaggttt gccagtccat ctccttgcgg gccaccccgg
     7681 tatgggcgaa cttcaagccg aacaaggaca tgccgccgcc gatgaccgcg ccgacgaact
     7741 tgtcattttt agaaaggtcg tcaatgatct tgtagagcgg ccagtaggat tgagcggcca
     7801 cggcgaaggc cagcatgaag gcgggcacgc ccagattgat gaggcccgtc aagactccgc
     7861 cgacgatggt cgtcttgggc cagaggttct cgcccgtgcc gaggccagcg ccgtagatgc
     7921 tggcgctgac ggcgttcggc ggaaacatga tgttgactac cagcttgccg gggtctaatc
     7981 gaatgggaat cgccatgatg ttgttgatgg gcatgatgtg cccgatgaat cgaaggtcgt
     8041 cgtactggat gtcgataact tcggtcatca cgttggcgac gaagttgtcg gacttccaat
     8101 cgggcaggct catggcgttg ccgttggcat cgtagaatct gatgtaagca ccgagatagc
     8161 gtatgtaaaa gttcgtcagc gtgagcttga gcgcccgcgc gccggggtat tgctcaatct
     8221 caaccttgaa gccgttaatc tcgcgcgtgt tggtcagaga gacgcgccag tcctttccag
     8281 actcggcctc aattcgcgcc gccagcgtca actggtctgg cccgttctca ttttcgacgc
     8341 tctggcccgt ctgctgttcc cacttcttgc cctccaatcg ctcgtcgttc ttcgtggcct
     8401 tcatcatggc cgtggcgaca gagcccgccg cttcgataat cgtgagcgtc ggctgggcga
     8461 aatagaaggt ttcgtccggg atgtgcgtct ccggatttgg ctcaggcgtg aagggttcca
     8521 atcgggccca gccggaattc tcagtgggtt ggcccatctg acgcatctgc atggcgaccg
     8581 cctgaaacat cgcatcaatc ttctgctcgt ccatgtactc gttaatgatg tgcgttactt
     8641 ccgggtcatt gttgatgagg tccggatgat gaaagatgag cgttttggcg tccgagaggt
     8701 aatccatcgc cgcatgg
//
LOCUS       DASWNS010000430         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_379694, whole genome shotgun sequence.
ACCESSION   DASWNS010000430 DASWNS010000000
VERSION     DASWNS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379694"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..1966
                     /locus_tag="VGB76_14965"
     CDS             386..1966
                     /locus_tag="VGB76_14965"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY0100249.1"
                     /translation="MDFDVIVIGSGFGGAISACRLAEAGYKVCILERGRRWNRDTYPR
                     KPDDPWLWNYDNPERQNGWLDLRVFPNMSVVQGAGVGGGSLLYANVSVEPPPDTFKDG
                     WPPEITYAELKPHYERVAKFMNVQKVPDRQWNPRMQLMKEGAEKIGRGDRFKLLPLAI
                     SFDPELTYDADHPLPVEASKRFVNAQGVEQGTCIHLGTCDIGCKVDARNTLDLNYIPW
                     AEKHGAEVRPLHLVNNIEQVAGGFRVSFDRLENRGRVAGSLTGRIVVVAAGSLGSTEL
                     LLRCRNVTKSLSQLSAFVGRNWSSNGDFLTPAIYAGREIHPTRGPTISTAIDFLDKSE
                     EGQSFWIEDGGFPDLLGYNMRRQAERPPQGRQAEMLVASIRDALRRDPFTNVMPWFAQ
                     AVDKADGTLSLKRKFLFWGAHRLNLSWNIAQSEPAINAVVNMHKRLSAATGGKPLVPP
                     GWALSRDLITPHPLGGCNMGSTPANGVVSHRGEAFNYKNLYVADAAIIPEALGVNPSR
                     TIGALAERIAKLIIEEGR"
     gene            2100..2510
                     /locus_tag="VGB76_14970"
     CDS             2100..2510
                     /locus_tag="VGB76_14970"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY0100250.1"
                     /translation="MAEGLEVKKSKIYGRGCFALKRFPARKKIAFYAGELIKGRRRIL
                     ARLNQQDAIKIISLNDDTAIDGAVGGDATSYINHSCAPNAFMRNMPGDRVAFFALRDI
                     EPGEEITMDYRDPDHPAVCRCGAPNCRSNKKSER"
     gene            complement(2593..>3326)
                     /locus_tag="VGB76_14975"
     CDS             complement(2593..>3326)
                     /locus_tag="VGB76_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100251.1"
                     /translation="ALPPAGQWVRLEVPASQVGLEGRTLNGMAFTLNGGRASWDRVGK
                     SASTSPTPTPTPTPTPTPTPTPTPTPTATPTPTPSPTGDVVWVEDAVPAGAATGGDSE
                     GWNWLGSNPPPFSGATAHQSNLVAGLHQHYFYNAASPLMINAGDKLVTYVYLDPANVP
                     SEIMLQWNDGTWEHRAYWGANVIPWGVDNTDSRRYMGALPPAGQWVRLEVPAAQLGLT
                     NRAINGIAFTLYGGRATWDRAGKSQ"
BASE COUNT          683 a         1019 c         1035 g          589 t
ORIGIN      
        1 gcaacatcaa aaaagggctg gtcagcaaat gagcgtgcgc gaatccgtcg gctgttgaag
       61 ggcggccaac ggattcgcac acacgacccg aagccacgcc gataggccgt ccaacgtcgc
      121 aactgaagtt gtctcattgt ccactgcgcg ccctgtaaat tgaagccctc ggaatgaagt
      181 attgacaatt taaccgcaca ggaatatttt gcctgcgttt ctcctgacgt gcgccccgct
      241 tcctctgtcg ccacacgtcc caacacctca gctttcccgt caggagaaag ccactcagca
      301 gttccaacag cggaaaggga gtccaacctt cggcgcagct aacggacggg cgtaaaactt
      361 ccactctcga agtgaggaca ccggcatgga cttcgatgtg attgtcatcg gcagcggttt
      421 cgggggagcg atcagcgcgt gtcggctggc ggaggccgga tacaaggtct gcatccttga
      481 gcgcgggcgg cgttggaaca gagacactta cccgcgcaag cccgacgacc cgtggctctg
      541 gaattacgac aatcccgaaa gacagaacgg ctggctcgac ctgcgcgtct tccccaacat
      601 gtccgtcgtg cagggcgcgg gcgtcggcgg cggctcgctc ctctacgcca acgtgtcggt
      661 cgaacctccg ccggacactt tcaaagacgg ctggccgccc gagatcactt atgccgaact
      721 gaagccgcac tacgaacgcg tggcgaagtt catgaacgtg cagaaggttc cggacagaca
      781 gtggaacccg cgcatgcagt tgatgaaaga aggcgcggag aagatcgggc gcggcgaccg
      841 ctttaaactg ctcccgctcg ccatctcctt cgatccggag ttgacctacg acgcggatca
      901 cccgctgccc gtcgaagcct caaaacgttt cgtcaacgcg cagggcgtcg agcaaggcac
      961 ctgcattcat ctcggcacct gcgacatcgg ctgcaaagtt gacgcgcgca acacgctcga
     1021 cctgaactat atcccctggg cggaaaagca cggcgcagag gtgcgaccgc tgcacctcgt
     1081 caacaacatc gaacaggtgg cgggcggctt ccgcgtctct ttcgaccgtc tggagaacag
     1141 ggggcgcgtc gcaggcagtc tcaccgggcg catcgtggtt gtggccgcag gctcgctcgg
     1201 ctccaccgag ttgttattgc gctgccgcaa cgtgacgaag agtttgtctc aactgagcgc
     1261 gttcgtgggg cgcaactgga gcagcaacgg cgactttctc acgcccgcca tctacgccgg
     1321 gcgcgagata cacccgacgc gaggcccgac catctccacc gccatagact ttctcgacaa
     1381 gagcgaagaa ggacaatcct tctggatcga ggacgggggc ttccccgatc tcttaggcta
     1441 caacatgcga cggcaggcag agcgtccgcc gcagggcagg caggccgaaa tgctcgtcgc
     1501 ctccatccgc gacgcactgc ggcgcgaccc gttcacgaac gtcatgccgt ggttcgcgca
     1561 ggcggttgac aaggccgacg gcacgctcag tctcaaacgc aagttcttat tctggggcgc
     1621 gcaccgtctg aacctgtctt ggaacatcgc ccaatcggaa cctgcgatca atgcggtcgt
     1681 caacatgcac aagcggctct ccgcggcgac tggcggcaag ccgctcgtgc ctcccggctg
     1741 ggctttgtcg cgcgacctga tcacgccaca cccgctcggc ggatgcaaca tgggaagcac
     1801 gcccgctaac ggcgtggtga gccacagggg agaagcgttc aactataaga acctgtacgt
     1861 ggcagacgcc gcgatcattc cggaggcgct cggcgttaat ccttcgcgca ccatcggcgc
     1921 gctcgcggaa cgaatcgcca agctcatcat cgaagaggga cgatagacgg cagcgtctga
     1981 acgcagcgac aggcgcgtcg gcaggcagac gatccgcccg gcctgttgcc gtgacgggcg
     2041 tactcatttt tgcagtatca tttttgcagt attgctcagg tcagggagag gagagttgaa
     2101 tggcagaggg gttagaagtc aaaaagagca aaatctacgg gcgcggttgc ttcgcgctca
     2161 aacgcttccc ggcgcgcaag aagatcgcgt tctacgcggg cgagttaatc aaaggccgcc
     2221 gtcgcatcct cgcgcgtctg aatcaacagg acgccattaa gatcatcagt ctcaacgatg
     2281 atacggcgat agacggcgcg gtcggcggcg atgccacgtc gtacatcaac cactcgtgcg
     2341 cgcccaacgc cttcatgcgt aacatgccgg gcgaccgcgt ggccttcttc gccctgcgcg
     2401 acatagagcc gggcgaagag atcacgatgg attacaggga tccggatcac cctgctgtgt
     2461 gcaggtgcgg cgcgcccaac tgccgctcca acaaaaagag cgaacgttag cttccgcctc
     2521 atagacaacg gcctgatgaa ctcaagagaa tatcgcttga gttcatcagg ccgttgacct
     2581 tgaagcgcag ggttattgag acttcccggc gcgatcccac gtggcgcgac cgccatagag
     2641 ggtgaaggct atgccgttga tggcgcgatt cgtcaggccg agttgcgcgg cagggacttc
     2701 aagcctgacc cactgaccgg cgggaggcag cgcgcccatg tagcggcgcg agtctgtgtt
     2761 gtctacgccc catgggatca cgttcgcgcc ccagtaggca cggtgctccc acgtcccgtc
     2821 attccattgc aacatgatct ccgacggcac gtttgccggg tctaagtaaa cataagtgac
     2881 cagtttatct ccggcgttga tcatgagggg gctggcggca ttgtagaagt agtgctggtg
     2941 caagcccgcg acgaggttgg actggtgcgc agttgcgccg gagaaaggcg gcgggttaga
     3001 gccgagccag ttccacccct cggagtcgcc gccggtggct gcgcctgccg gtacggcgtc
     3061 ttcgacccag accacgtcac ccgtaggcga aggcgtcggc gtgggagtcg cagtaggagt
     3121 cggcgtcggg gtcggcgtag gagtcggtgt tggcgtcggg gtcggggtcg gcgtgggtga
     3181 tgttgacgca gacttgccga cacgatccca actggcgcga ccgccgttga gcgtgaaggc
     3241 catgccgttg agcgtgcgac cttcgagtcc gacctgactg gcagggactt caagcctgac
     3301 ccactgaccg gcgggaggca gcgcgc
//
LOCUS       DASWNS010000431         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_456700, whole genome shotgun sequence.
ACCESSION   DASWNS010000431 DASWNS010000000
VERSION     DASWNS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456700"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2259)
                     /locus_tag="VGB76_14980"
     CDS             complement(<1..2259)
                     /locus_tag="VGB76_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0100252.1"
                     /translation="MFRESIYKFTFGVALAFAFCASVMAQSHIGTVQGTVRDPSGALV
                     PGASVRIADPTSGYQQTAQTDEQGEYKFFNVPFHIYTLRVEANGFQTAEKSFDVETAI
                     PVNIDITLSVAAASEIVTVTENASLIEADKTSSDTNISQTLLERPLGATPSRGIEAIV
                     ASAPGFAPDDNGRLHPRGSESQVQYVVDGVPITDNLSAIFATSLDARTLRTVEVLTGG
                     ISAEFGDKLGGVINVNTRSGREMPTQGSVTLSGGSFSTGEIGVNFSTRTDRLGLLTNL
                     SASTSQRFLDPPTIENFHNFGRTGKGFLRLDYQFTPNDTLRATFLAGGTNFQVANRLA
                     QEIAGQDQRQRLRDHSEFITYERIFSPNTFGQFSFFNRYGTARLTSNPFSTPVAAFQD
                     RSLQNTGGIAAISFTRGRHNLKVGGQFTITPIAEKFSFYPTAPFEDFTNEDGETVPNP
                     INTFTPANPFRFEGNRRGRLLSAYIQDRFSPFRNFTIDAGVRYDNYKLIIQEDAFSPR
                     IGVAYFIPRTQTTLRASYNRIFQTPPAENLLLASSAEAAALSPLAVVQGQEGVRPILP
                     DKQHVYEVGVQQAASRYMRLNLTVYQKRITNFGDKDQFFDTGIIFPITISSGRVTGAE
                     MRLETADLRGFRAFASYANSRAYGVTPINGGLFLGEAVESLETPGLKFANDHDQRNSA
                     QFQLSYNHARSGLYAIFGGRYDSGYPTDVEPGTTPQDFAAAGFDPRLYNEIDFARGRT
                     KPRTLLNFSIGAD"
     gene            complement(2407..>2614)
                     /locus_tag="VGB76_14985"
     CDS             complement(2407..>2614)
                     /locus_tag="VGB76_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100253.1"
                     /translation="NNFRTVFFRQLPSRCNRPQTASGCSRSPLTTTLLRTIRRADALH
                     PSLLFSKFIFHFERFAILTKFSKR"
BASE COUNT          503 a          681 c          906 g          524 t
ORIGIN      
        1 gtccgcgccg atggagaaat tgagcagggt gcgcggcttg gtgcgcccgc gcgcgaaatc
       61 aatttcgttg tagaggcgcg ggtcgaagcc tgcggcggcg aaatcttggg gcgtcgtgcc
      121 gggttcaacg tcggtcgggt agccggaatc gtagcgaccg ccgaagatcg catacagacc
      181 ggaacgcgcg tgattgtaac ttaactggaa ctgcgcggaa ttgcgctggt cgtggtcgtt
      241 ggcaaacttc agaccgggcg tttcgagact ctcaaccgct tcgccgagga atagcccgcc
      301 gttaatcggc gtcacgccgt aggcgcgcga gttggcgtag gaggcgaaag cgcggaagcc
      361 gcgcaggtcg gcagtctcaa ggcgcatctc cgcgcccgtg acccgaccgg atgagatcgt
      421 gatgggaaag atgatgcctg tgtcgaagaa ctgatccttg tcgccgaagt tggtgatccg
      481 tttctgatag acggtcaggt tcagccgcat gtagcgcgag gcggcttgtt gcacgccgac
      541 ctcgtagacg tgctgcttgt ctggcaggat cgggcggacg ccctcctgac cctgcaccac
      601 ggcgagcggc gacaaggcgg cggcctctgc ggaactggcg agcagcagat tctctgcggg
      661 cggcgtctgg aagatgcgat tgtaggaggc gcgcagggtc gtctgtgtgc gcgggatgaa
      721 gtaggccacg ccgatacgcg ggctgaaagc gtcttcctga atgatcagtt tgtaattgtc
      781 gtagcgcacg cccgcgtcaa tcgtgaaatt gcgaaacggc gagaagcggt cttggatgta
      841 cgcgctcaac aggcgacccc tccggttgcc ctcgaagcga aaagggttgg cgggggtgaa
      901 agtgttgatc gggttcggca ctgtctcgcc gtcttcattc gtgaaatctt caaagggcgc
      961 ggtcgggtag aaactgaact tctcggcgat gggcgtgatc gtgaactggc cgccgacctt
     1021 caaattgtgt cttccgcgcg tgaacgaaat ggccgcgatg ccgcccgtgt tttgcagtga
     1081 gcgatcctga aacgccgcga ccggcgtgga gaaaggattg ctcgtcaggc gcgccgtgcc
     1141 gtagcggttg aagaacgaaa attgaccgaa ggtgttgggc gagaagatgc gttcgtaggt
     1201 gatgaactcc gaatgatcgc gtagacgctg ccgctgatcc tgacctgcga tctcctgtgc
     1261 gagccggttc gccacctgaa aatttgtgcc gcccgcgagg aacgtggcgc gcagcgtgtc
     1321 gttcggcgtg aattgatagt cgagccggag gaaacctttg ccggtgcgcc cgaagttgtg
     1381 aaagttttcg atggtgggcg gatcgagaaa acgctgagag gtggaggccg ataaattcgt
     1441 caacagtccg agcctgtctg tgcgggtgga aaagttgacg ccgatttcgc ccgtggaaaa
     1501 actgccccct gataaggtga cgctgccttg cgtgggcatc tcgcgcccgg aacgcgtgtt
     1561 gacgttgatg acgccgccga gtttatcgcc gaactctgcg gagatgccgc ccgtcaggac
     1621 ttcaacggtg cggagcgtgc gcgcgtcgag gctcgtggcg aagatggccg agaggttgtc
     1681 cgtgatgggc acgccgtcca cgacgtattg cacttgcgat tcggagccgc gcgggtgaag
     1741 ccgcccgtta tcgtcgggcg caaaaccggg cgcgctcgcc acgatagctt cgatgccgcg
     1801 cgagggagtt gcgccgagcg ggcgttcgag taacgtctgg gagatgttcg tgtccgaaga
     1861 ggtcttatcg gcttcgataa gcgaagcgtt ctccgtgacg gtcacgattt cgctcgcggc
     1921 ggcgaccgag agcgtgatgt cgatgtttac ggggatcgcc gtctcaacgt cgaaggattt
     1981 ttcggcggtc tggaagccgt tcgcttcgac gcgcagggtg tagatgtgaa acgggacgtt
     2041 gaagaatttg tactcgccct gttcatctgt ctgcgccgtc tgttggtaac cgctcgtcgg
     2101 gtcggcgatg cgtaccgacg cgccgggcac gagcgcgccg ctcgggtcgc gcactgtccc
     2161 ctgcaccgtg ccgatgtgtg actgcgccat gacggaagcg cagaaggcga aagccagcgc
     2221 tacgccgaag gtgaatttat aaatgctttc tcgaaacata agagcctctc ctcgttgccg
     2281 tgcggcctgc gcctccggcg ttgattgacg gcatgtgccg cctgacgcct gaccggaaat
     2341 cgtgccggcc tccggtttat gaaaacggac gagggcagcg acgataggct tgagcgtctt
     2401 aaatctttaa cgcttcgaga acttcgtcag gatggcgaag cgttcgaagt ggaaaatgaa
     2461 tttagagaag agaagcgagg ggtggagcgc gtccgcgcga cgaatcgtgc ggagcagagt
     2521 agttgtcaga ggagagcgcg agcagccgga tgctgtttgc gggcggttgc agcgcgaagg
     2581 gagttgcctg aaaaagaccg tgcgaaagtt gttg
//
LOCUS       DASWNS010000432        11767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_68092, whole genome shotgun sequence.
ACCESSION   DASWNS010000432 DASWNS010000000
VERSION     DASWNS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68092"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2185)
                     /locus_tag="VGB76_14990"
     CDS             complement(<1..2185)
                     /locus_tag="VGB76_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100254.1"
                     /translation="MPEANTREHSGDAGESISVKRALLHGLFVVAFYTLLFVLFFAPV
                     LFSAYLLAPGDGILYFLPNFYARRVWWDASVWGGFPAVGDSQLMLWYPPAILFALVKS
                     WNAFLLSAYVLAASFTYAYVYALTRSRLAATFAGTCYGMSGFFIAHLGHAALVHAAAW
                     LPMFILSLEMLAGRRDAGGLRGGELRGVVEPESSDESKNGNESGSVSESRVGESRVGF
                     SSFWFTVGVLTVACCALAGHPQIFVYTLSLGGAYVLVRGWQVAAGRWLYYFLCALLVL
                     TGTGLACVQLVPTAELAALSLRASLGFAEFVSYALPPGQTPMLLFPYLYGGSPGTLYD
                     LPYFGAWGSEAGGWGAGELSGYVGLLPLLLAALGFFTTRRRRHSLAWFWLAVCAVAFL
                     LALGDATPLARLTYRLPVINKFRVPARHFMELALGVSVLAGYGAHAVLEKAASSRLLN
                     RTLAVAAALMLACLLAVIALTDELNASAAATLKRPLSFLPWANPATGVPLLVLLAAAA
                     TLLYWHRRPHSTLRGALLVAVLALDLASFNWFYEWHYGAPYKVFQSPPLAALRFRDEL
                     DATHQRLLPVRGGTGRVSEIPPDLSKLWNFPSASGYGPFILSRVSRLLTMPPHGSVND
                     AWRDPSNLSLDMMAVRYLFVPRAGIEPPAITDEQGIKWTPNEFNVLLGSGCAAPNPAI
                     FKYTLPQTARADSVAIATALACSVALPDQAEVANVMVRDTRGQTYT"
     gene            complement(2221..3156)
                     /locus_tag="VGB76_14995"
     CDS             complement(2221..3156)
                     /locus_tag="VGB76_14995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0100255.1"
                     /translation="MSSTQTKPEPPAPAQSAPADDPAASGNVAATNNHCLVCDTNEAA
                     PLYPGILKCRNCEYVFADMRLTDEELFNLYNEEFFTGAEFSDYASDAKFFRKNFRLRF
                     RELKKFLSPSRHKKLLEIGSAYGFFLDVVRDEFQSVQGIDITDAGVRFAREELQLDVV
                     QADFLAHDYGAQKFDVVCMWDTIEHLREPQRYVEKIAAHTAPGALFALTTADIDSLNA
                     KLRKDKWRMIHPPTHLHYFSTKTIARLLDAHGFDVVYNRYCGFYRSIGNMAYNILVLR
                     QQKPALYRPLERLGVGGLGFYLNLYDIMYVIARRR"
     gene            complement(3153..4208)
                     /locus_tag="VGB76_15000"
     CDS             complement(3153..4208)
                     /locus_tag="VGB76_15000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0100256.1"
                     /translation="MSSTTSETKATKTKPATRAVVESRLKTTTTAASANAAESSPVLA
                     IVVPCLNEELVIRDTVRRLLEVLQKLTVAGKVDAESFVYIVDDGSSDATWEAIEELHA
                     NDARVKGLKLVRNVGTQNALLAGLLSVKEHADCVVTIDADLQQDEAVIDTFLEKYRAG
                     AHIVYGVRKGRADSYLKKATSSFFYNLMKLMGAKIIKHHSEYRLVSRKAIEAISEYRE
                     YNLFLRGIFVDIGYKSEIVPFTVRRRGAGTSKHNFRKLSSLALDGITSFSIVPLRLVT
                     VAGMLVFLFSVLMTLFYLYHKIKGHTPPGWAGTIIPIYFLGGVQIMFLGIVGEYIGKI
                     YQEAKSRPRYIKDTELF"
     gene            complement(4212..6665)
                     /locus_tag="VGB76_15005"
     CDS             complement(4212..6665)
                     /locus_tag="VGB76_15005"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0100257.1"
                     /translation="MKPLAIVIPWFGAELKGGAEQQAFQIATRLAARGASVEVITTCC
                     RSFQDDWATNHLPPGVTEEHGLTVRRFPVGTRDREAFEQVNAKLLALTPLELRAGVSP
                     LDARDAEIFAAQNINSPALLEHLRARGDDYRAFIFIPYMFAPTTAGLPLVAERAFLQP
                     CLHDEPAAYLPVVENLFQRARLVLFNSDGERELAARLYGGASVFPQSMVVGEGIERAA
                     FTEQQLADALPTELRAGAPFVLYLGRRDEVKNTGLLVRAFRRFKERAQTNNETSYEPN
                     NERRLKLVLAGAGAHSYDERERDILDLGLVSEETKAALLFACRALFQPSRNESFSRVL
                     MEAWSYGGRPVAAHRECLATSIAVERAGGGWLAATEEEWTHTFAHVVSLPETELARMG
                     ARGRRYADEHADWDKVIARYEELLNDELLNEELSNSSAQAGDTPAPETLHGSFAARNA
                     GQSFAAVHQLLPDIVYGDAISGQAIQIRDYLRSRGHASEIFVKRRDERLEQEARLFDP
                     ALVKPTDALIYHHSIGSELTAFAVAHAGAKCLVYHNITPAEYYAPYRPGFAWMLETGR
                     AHLPRLAPHFDCSVGDSSFNAAELAACGFPHPGVLPIICDPAKWKLRADPCVMERLQD
                     GRVNLLFVGRIAPNKKQDELVRAFAHYRELEADARLVIAGEGRASDPFYTRLLADIAA
                     RGLSGHVIVTGQVTDAELLAYYRTAHLYWSLSEHEGFGVPLVEAMWFDVPVLAYRSTA
                     TPETLKDAGVTFESKDDLRAIASLARRLTRDDRDLRRRVIAAGRVRREAFTPARVHSI
                     LDELLAQMESLTARREEVA"
     gene            complement(6682..7815)
                     /locus_tag="VGB76_15010"
     CDS             complement(6682..7815)
                     /locus_tag="VGB76_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100258.1"
                     /translation="MSEELMIEVDDTKRGASDPATTQERDGAATSAGTSTPPGDATGE
                     TTSEAIGDVAGDANGDVTRPPVEDARDIEAHVLPLEVEARGKVHYRVEELLQYHDVHF
                     IENAYVALLERAPSDAERARELDELRSGRASKVEIVERLASRPEATGRGVRVEGLPSP
                     VMRRLSHVPVVGYVLRLARALVRLPLLMEHQQQFEIYALAQQQILTDRVEQLSALPAQ
                     GNVVAVGAEAQGVQNSGSPVQADIIATVEMFSDALLDLSNGHAELQAQVQTQAEQVQS
                     ALTNLTQAVTTQQQITETLRREQHAAADAQQEFLIQEQRVIVETQKVVLEELREELRA
                     LSQRQQEARAQLEAELRRLQTLPDATATTAAATTTPREDFSGR"
     gene            complement(7812..9218)
                     /locus_tag="VGB76_15015"
     CDS             complement(7812..9218)
                     /locus_tag="VGB76_15015"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0100259.1"
                     /translation="MSTALRVEGVAKQYRIYDHPSDRLKETLTRGWLKRHREFWALRE
                     ISFEIERGTTVGIVGANGSGKSTLLQIITGTLEPTHGDVQVEGRVAALLELGAGFNLE
                     FTGLENIYMNTALMNFSRAQTDARLAGIERFAEIGDFLHQPVKTYSSGMYVRLAFAIA
                     VNTDPEILVVDEALSVGDTIFQHRCVRRIKEMQEAGTTILFVSHEPTLVRALCSRAIL
                     LNAGRMIADGTPMEVLNRYQRVIMAREEAYAGATGGEAAVENDAGENESAQGSVAGVA
                     HVAGGGEAAVVRQPLQYSYRHGNGRAEVIGAELFDVATNRSVELIETGDAVAVRLRIL
                     FKEDVAEPVCGFMIRNRHGINVYGTNTEQRGRPLGAARRGEIIEAVFTFNCWLGQEHY
                     FVSVAAHTPDGEAFDWLDGVIFFRVSCAVEMEGIANLNAEVTARRVQAEAGGGVDAQT
                     LQTDENASGREETRAVKA"
     gene            9366..10190
                     /locus_tag="VGB76_15020"
     CDS             9366..10190
                     /locus_tag="VGB76_15020"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0100260.1"
                     /translation="MISAQDSRRLVWRPLWELPGRAELIRALVRRELAARYRGSALGV
                     LWAMLTPAVMISIYTFIFAGIFGARFGERGTAWDYALYLFCALLPWTAFQDSLQVSSN
                     VIVAHANLVKRVVFPLEALPVAQVMAALTTQMLGTIVLLLATLIIQRELHLTLLWLPV
                     VVLPQLVLMLGAAWLVASLGVFLRDTGQVVSLLVVAWMFLTPIIYPEQIVPVRYQGFI
                     NANPFTPLVRNYRRIILEGVAPDWRGLAYFTLFALVVFILGYWWFAKTRKNFADVI"
     gene            complement(10298..>11767)
                     /locus_tag="VGB76_15025"
     CDS             complement(10298..>11767)
                     /locus_tag="VGB76_15025"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0100261.1"
                     /translation="LRLAEATAFGGLLLYAVFAPHSIAGAWIALSVSISGWLARTLFT
                     RRTNLRRTALDLPLWLFYAWSILSALFSAEPRISLLKLISVSTFLILYFVQSLITRRM
                     AAVCAALMIASGVAGALWSAFEVARGRGLVIDEIAADSPLRSPSLGVAAGDTLWRVNE
                     RRVNTVAEIDQAIRDAPVGEPLRLSLFSQGEQAERVGTERVSGDLKARPSPSGLRGTR
                     RSHRFRASGWTRHYQTFAETLQILAQLSLGFALAHWQRRRTCPLPRLRFALFAAAFLC
                     LAAGIALTAMRTALVAFAIGACVVAWRATSRVRTRFVITVIVAFAVALGAFAVWRTRA
                     EGALRLQDESSRLRLAVARVALTRTPEHPLFGHGMDAVKRHWTEWGFPGEVQIHTHST
                     PLQLAFERGLPALLLWLWLLAVFWLTLTRGERAWRASADASTHGLLLGATGAFAGFCA
                     SSLVNYNFGDAEVALVLWWLMGTVVKISGDTQKASGSRP"
BASE COUNT         2114 a         3633 c         3873 g         2147 t
ORIGIN      
        1 gcgtgtaggt ttgaccgcgc gtgtcgcgga ccatgacgtt tgcgacttcg gcttgatccg
       61 ggagggcgac ggagcaggcg agcgcggtgg caatggcgac ggaatcggcg cgcgccgttt
      121 gcgggagcgt gtatttgaaa attgccggat tcggcgcggc gcaaccggaa ccgagcagca
      181 cgttgaattc gttgggcgtc cacttgatgc cctgctcgtc cgtgatggcg ggcggttcga
      241 tgcctgcgcg cggcacgaag aggtagcgca cggccatcat gtcgagactg aggttggagg
      301 ggtcgcgcca tgcgtcgttg acgcttccgt gcggcggcat cgtcagcagg cgactgacgc
      361 gcgagaggat gaatggacca tagccgctcg cgctcggaaa attccagagc ttcgagaggt
      421 cgggcggaat ctcgctgacg cgccctgtgc cgccgcgcac ggggagcagc cgttgatgcg
      481 tggcgtcgag ttcgtcgcgg aaacgcagag ccgcaagcgg cggcgactgg aagactttat
      541 agggtgcccc gtagtgccat tcgtaaaacc agttgaagct ggcgaggtcg agcgcgagca
      601 cggcgacgag gagcgcgccg cgcagggtgg agtgtggccg cctgtgccag tagaggagcg
      661 tggccgccgc cgcgaggagg acgaggagcg gcacgcccgt cgccggattc gcccacggga
      721 gaaaactgag cggacgcttc aacgtcgcgg cagccgacgc gttcagttcg tcggtcaaag
      781 cgataactgc gagcaggcag gcgagcatca gggcggcggc cacggcgagc gtgcgattca
      841 gcagccgact cgaagcggct ttctccagaa cggcgtgcgc gccgtagccc gcgagcacgc
      901 tcacgccgag cgcgagttcc atgaagtggc gcgcgggcac gcgaaacttg ttgatgacgg
      961 gcaggcgata ggtgagacgt gcgagcggcg tggcgtcgcc gagtgcgagc aggaaggcaa
     1021 cggcgcacac ggcgagccag aaccatgcga ggctgtgtcg tcgtcgccgc gtcgtgaaga
     1081 agccgagggc ggcgagcagg agcggcagca gccccacata gccgctgagt tcgcccgcgc
     1141 cccatccgcc cgcctccgaa ccccacgcgc cgaagtaggg caggtcgtag agtgtgccgg
     1201 gcgagccgcc atagagatag ggaaagagca gcatcggcgt ctgccccggc ggcagagcgt
     1261 aagagacaaa ctccgcgaag ccgagcgacg cgcgcaaact caaggcggcg agttccgccg
     1321 tcggcactaa ctgcacgcag gcgagtcccg ttcccgtcaa gaccagaagc gcgcagagga
     1381 agtagtagag ccaacgcccc gccgcgacct gccagccgcg cacgagcacg tatgcgccgc
     1441 cgagcgagag cgtatagacg aagatttgcg gatgccccgc gagcgcgcag cacgccacgg
     1501 tgagaacccc gaccgtgaac cagaaactac tgaaccccac gcgcgactca cccacgcgtg
     1561 actcactgac gcttcccgac tcgttgccgt ttttcgactc gtcgctgctc tccggctcga
     1621 cgaccccgcg caactcgccg ccgcgcaatc caccggcgtc tctccttccg gcgagcatct
     1681 ccagcgacag gataaacatc ggcagccacg cggcggcgtg gacgagcgcg gcgtgtccga
     1741 gatgtgcgat gaagaagccg ctcatgccgt agcaggtgcc cgcgaaggtc gcggcgaggc
     1801 gcgaacgggt cagggcgtag acataagcgt aagtgaaact ggcggcgaga acgtaagccg
     1861 agagcagaaa ggcgttccac gatttgacga gcgcaaacag gatcgcgggc ggataccaca
     1921 acatcaactg cgaatcgccc accgccggaa agccgcccca cacggacgcg tcccaccaca
     1981 cgcgccgcgc gtaaaagttt ggcaggaaat agaggatgcc gtcgcccggc gcgagcaggt
     2041 aagcggagaa gagaacgggc gcgaagaaga gcacaaagag cagcgtgtag aacgcgacaa
     2101 cgaacagtcc atgcagcaac gcacgcttca cggatattga ctctccggcg tcgccggaat
     2161 gttcgcgcgt gttcgcttca ggcattcagt cttaaagtac gcggagctta tttgccatct
     2221 tcaacgacgc cgcgcgatga cgtacatgat gtcgtagagg ttgagataga agccaaggcc
     2281 gccgacgccg aggcgttcga gcgggcggta gagcgcgggc ttttgctggc gcaggacgag
     2341 gatgttgtag gccatgttgc cgatgctgcg atagaagccg caatagcgat tgtagaccac
     2401 gtcgaagccg tgtgcgtcga gcaggcgggc gatagtctta gtcgaaaaat aatggaggtg
     2461 cgtgggcggg tggatcatgc gccacttatc ttttctgagt tttgcgttga gactgtcgat
     2521 gtcggcggtg gtgagcgcga agagcgcgcc cggtgcggtg tgcgcggcga tcttctccac
     2581 atacctttgc ggctcgcgca ggtgttcgat ggtgtcccac atgcagacca cgtcgaactt
     2641 ctgcgcgccg taatcgtgcg cgagaaaatc ggcctgcacc acgtcgagct gcaactcttc
     2701 gcgcgcaaag cgcacgcccg catccgtgat gtctatgccc tgcacgctct ggaattcgtc
     2761 gcgcaccacg tcgaggaaga agccgtacgc gctcccgatc tcaagcagtt ttttatggcg
     2821 cgacggcgag aggaattttt ttagttcgcg gaagcgcaaa cggaaattct tgcgaaagaa
     2881 cttcgcgtcc gaagcgtagt cgctgaactc cgctcccgtg aagaactctt cgttgtagag
     2941 gttgaaaagt tcctcgtccg tcaggcgcat gtcggcgaag acgtattcgc agttgcgaca
     3001 tttgaggatg ccgggataga gcggcgcggc ctcgttcgtg tcgcagacga ggcagtgatt
     3061 gttggtcgcc gcaacgttgc ctgacgccgc cgggtcgtcg gcgggcgcgg attgggcagg
     3121 ggcgggcggc tccggtttcg tctgtgtgga actcaaaaca gttcggtgtc cttgatgtag
     3181 cgcgggcgcg atttggcctc ttgataaatc ttgccgatgt attcgccgac gatgccgaga
     3241 aacatgatct gcacgccgcc gagaaagtag atcgggatga tcgtgcccgc ccagccgggc
     3301 ggcgtgtgcc ctttgatctt gtgatagaga taaaagagcg tcatcagcac gctaaacaga
     3361 aagacgagca tcccggccac cgtcacgagc cgcaggggaa cgatgctgaa cgaagtgatg
     3421 ccgtcgagcg cgagcgacga gagcttgcgg aagttgtgtt tcgaagtccc cgcgccgcgc
     3481 ctgcgcactg tgaacggaac gatctcggac ttgtagccga tgtccacgaa gatgccgcgc
     3541 aggaaaagat tgtattcacg atattccgag atggcctcga tggccttgcg ggaaacgagc
     3601 ctgtattccg agtggtgctt gatgatcttc gcgcccatca gtttcatcag gttgtagaag
     3661 aaactcgaag tcgctttctt gaggtaagag tcggcgcgac ctttgcgcac gccgtagacg
     3721 atgtgcgcgc ccgccctgta cttctccaaa aacgtgtcga tgaccgcttc gtcctgctgc
     3781 aaatcggcgt caatcgtgac gacgcaatcg gcatgttctt tgacgctcag gagtcccgcg
     3841 agcagggcgt tctgcgtgcc gacgttgcgc acaagcttga gtcctttgac gcgcgcatca
     3901 ttcgcgtgga gttcttcaat cgcctcccag gtcgcatcgc tgctcccgtc gtccacgatg
     3961 tagacgaaac tctccgcgtc aactttcccg gcgacggtca gtttttgtaa aacttccagc
     4021 agccgccgta cggtgtctct gatgacgagt tcttcgttga gacagggcac gacgatggcg
     4081 agcacggggc tggactccgc agcgttcgcg gacgcagccg tcgtcgtcgt cttcaggcgc
     4141 gactcgacga cggcgcgcgt cgcgggcttc gtctttgtcg ctttagtctc gctcgtcgtc
     4201 gaactcacgc cttatgctac ctcttctcgc cgcgccgtta aactctccat ctgcgccagc
     4261 aactcgtcca gaatggaatg cacgcgcgcg ggcgtgaaag cctcgcgccg cacgcgcccg
     4321 gcggcgatga cgcgacggcg caaatctctg tcgtcgcgcg tgagccgtct ggcgagagag
     4381 gctatggcgc gcaggtcgtc tttgctctca aacgtcaccc cggcatcctt gagcgtctcc
     4441 ggcgtcgccg tgctccggta ggcgaggacg ggcacgtcga accacatcgc ttcgacgagc
     4501 ggcacgccga agccttcatg ttcgctcaaa gaccagtata ggtgcgccgt gcggtagtag
     4561 gccagcagtt cggcgtccgt gacctgccct gtgacgatga cgtgcccgga gaggccgcgt
     4621 gcggcgatgt ctgccaggag gcgcgtgtag aaggggtcgg aagcgcgccc ttccccggcg
     4681 atgacgaggc gcgcgtcagc ctccaactcg cggtaatgcg cgaaggcgcg gacgagttcg
     4741 tcctgcttct tgttcggcgc gatgcgcccg acgaagagca ggttgactcg cccgtcctgc
     4801 aagcgttcca tcacgcacgg gtctgcgcgc aacttccact tggcggggtc gcagatgatc
     4861 ggcagcacgc cggggtgcgg aaagccgcag gccgcgagtt ccgccgcgtt gaaagatgag
     4921 tcgccgaccg agcagtcgaa gtggggcgcg aggcgtggca ggtgcgcgcg tcccgtctcc
     4981 agcatccagg cgaagccggg gcgatagggc gcgtaatatt cagccggagt gatgttgtga
     5041 tagacgaggc atttcgcgcc cgcgtgtgcg acggcaaagg cggtgagttc cgagccgatg
     5101 gaatgatgat agatgagcgc gtccgtgggt ttgacgagcg cgggatcgaa cagccgcgcc
     5161 tcttgctcca aacgttcgtc gcgccgcttg acgaaaatct ccgaggcgtg gccgcgactt
     5221 cgcaggtagt cgcgaatctg tatcgcctga ccggagatcg cgtcgccgta gacgatgtca
     5281 ggcagtagct ggtgaaccgc cgcgaaactt tggcctgcat ttcgagccgc gaaacttccg
     5341 tgcaaagttt ccggtgcggg ggtgtcgccc gcctgtgcgc ttgagtttga tagttcctcg
     5401 tttaacagtt cgtcattcaa cagttcttcg taacgcgcga tgaccttgtc ccaatccgcg
     5461 tgctcgtctg cgtagcggcg accgcgcgcg cccatgcgtg ccagttccgt ctccggcagc
     5521 gacacgacgt gtgcgaacgt gtgcgtccat tcttcttccg tcgccgcgag ccagccgccg
     5581 cccgcgcgct cgacggcgat tgaggtggcg aggcattcgc ggtgcgcggc cacgggacga
     5641 ccgccgtaac tccaagcctc catcaacacg cgggaaaaac tttcgttgcg gctcggttga
     5701 aagagcgcgc ggcaggcaaa gagcagcgcg gctttcgtct cttcgctgac gaggccgaga
     5761 tcgagtatgt cccgctcgcg ttcgtcgtag gaatgtgcgc ccgcgcccgc gagcaccagt
     5821 tttagacgcc tctcattatt cggctcataa ctcgtctcgt tattcgtctg agcgcgctcc
     5881 ttgaagcgtc ggaaggcgcg gacgagcagt cctgtatttt tcacttcgtc gcggcggcct
     5941 agatagagca cgaagggcgc gcccgcgcga agctccgtgg ggagcgcgtc cgcgagttgc
     6001 tgttcggtga aggcggcgcg ctcgatgcct tctccgacca ccatgctctg cggaaagacg
     6061 gacgcgccgc cgtagagacg cgcggccagt tctcgctcgc cgtccgagtt gaagagaacg
     6121 aggcgcgcgc gttggaacag gttttcgacg acggggagat aagctgcggg ttcgtcgtgc
     6181 aggcagggct gcaaaaaggc tcgttcggcc acgagcggca gcccggcggt ggtcggcgcg
     6241 aacatgtaag ggatgaagat gaacgcacgg taatcgtcgc cgcgtgcgcg caggtgttcg
     6301 agcagcgcgg gcgagttgat gttctgcgcg gcgaagattt cggcgtcgcg cgcgtcaagg
     6361 ggagagacgc ccgcgcgcag ttcgaggggc gtgagcgcga gcagttttgc gttgacctgt
     6421 tcgaaagcct cccggtcgcg cgtgccgacg gggaagcgac ggacggtgag tccgtgctct
     6481 tcggtgacgc ccggcgggag atgattcgtc gcccagtcgt cttgaaagga gcggcagcag
     6541 gtggtgatga cttcgacgct tgcgccgcgc gccgcgagcc gggttgcgat ctgaaaggct
     6601 tgttgctcgg ctccgccttt caactccgcc ccgaaccagg ggatgacgat ggcgagcggc
     6661 ttcatctttt catccccttt atcatctccc cgaaaaatct tcgcgcggag ttgtcgttgc
     6721 cgctgcggtt gttgccgtgg catccggcag cgtctgcaag cgtcgcagtt ccgcttcgag
     6781 ttgcgcgcgt gcctcctgtt gcctctgcga gagcgcgcgc aattcctcgc gcaactcttc
     6841 gagcaccacc ttctgcgtct cgacgatcac gcgctgttcc tgaatcagaa attcctgctg
     6901 cgcgtcggct gccgcgtgtt gctcgcgccg gagcgtttcc gtgatctgct gttgcgtcgt
     6961 gacggcctgc gtcaagtttg tgagcgcaga ctgcacctgc tcggcctgcg tctgcacctg
     7021 cgcctgcaac tcggcgtgac cgttcgacag atcgaggagc gcatcggaaa acatctccac
     7081 cgtggcgata atatcggcct gcaccggcga gccggagttt tgcacgccct gcgcctcggc
     7141 tcctacggcg acgacgtttc cctgcgcggg cagcgcggaa agttgctcga cgcgatctgt
     7201 gagaatctgt tgctgcgcga gcgcgtagat ttcaaactgc tgctggtgct ccatcaacaa
     7261 gggcagacgc acaagcgcgc gtgcgagccg cagcacgtaa ccgacgacgg gcacgtgact
     7321 caaacgccgc atcacagggg acggcaagcc ttcaaccctc acgccccgcc cggtcgcttc
     7381 cggtctggag gccagacgct cgacgatctc caccttactc gcgcgcccgc tccgcaactc
     7441 gtccaactcc cgcgcacgtt ccgcgtcgga aggcgcgcgt tcgagcagcg cgacgtaagc
     7501 gttctcgatg aaatgcacgt cgtgatactg caacagttct tcgacgcgat aatgaacttt
     7561 gccgcgcgcc tcgacctcaa gcggcaggac gtgagcttcg atgtcccgcg catcttcgac
     7621 aggcgggcgc gtcacgtcgc cgttcgcatc gccagccaca tcgcctatcg cctcgctcgt
     7681 cgtctcgccc gtagcgtcgc cggggggcgt gctcgttccg gcagaagtcg ccgcgccgtc
     7741 gcgctcctgc gtcgtcgccg gatcgctcgc gccgcgtttc gtgtcgtcca cttcgatcat
     7801 caattcttcg ctcaagcctt cacggcgcgc gtctcctcgc gcccgctcgc attctcgtcc
     7861 gtttgaagtg tttgcgcgtc aacaccgccg cccgcttccg cctgcacgcg tcgcgccgtc
     7921 acctccgcgt tgagattcgc gatgccctcc atctccaccg cgcacgagac gcgaaagaag
     7981 atcacgccgt cgagccaatc gaaggcttcg ccgtccggcg tgtgcgcggc gaccgagacg
     8041 aaataatgct cctgtccgag ccagcaattg aacgtaaaga cggcttcgat gatctcgccg
     8101 cgacgcgccg cgccgagcgg tcgcccgcgc tgctccgtgt tcgtcccata gacgttgata
     8161 ccgtggcgat tgcgaatcat aaacccgcac acgggttcgg ccacgtcctc tttgaacagg
     8221 atgcgcaggc gaactgccac cgcgtcgccc gtctcgatca actcgacgga acggttcgtc
     8281 gccacgtcga acaactccgc gccgataacc tccgcgcgcc cgttcccatg acgatacgaa
     8341 tactgcaaag gctgacgcac gaccgctgcc tcgccgccgc ccgcgacgtg cgcaacgccc
     8401 gcgacgctgc cctgcgcact ctcattttcg ccagcatcat tctcaaccgc cgcctcgccg
     8461 cccgtcgcgc ccgcataagc ctcttcgcgc gccatgatca cgcgctggta acgattgagc
     8521 acttccattg gcgtgccgtc ggcgatcatg cgcccggcgt tgaggaggat cgcgcgggaa
     8581 cagagcgcgc gcacgagcgt cggttcatgc gagacgaaga ggatggtcgt gcccgcctcc
     8641 tgcatctcct tgatgcgccg cacgcagcga tgctgaaaga tcgtgtcgcc gaccgacaga
     8701 gcttcgtcca cgacgagaat ctccgggtct gtgttgacgg cgatggcgaa ggcgagccgc
     8761 acgtacatgc cggaggaata ggtcttgacg ggctggtgta gaaagtcgcc gatctctgcg
     8821 aagcgttcga tcccggcgag gcgtgcgtcc gtctgagcgc gcgaaaaatt catcagcgcg
     8881 gtgttcatgt agatgttttc gaggccggtg aattcgagat tgaagcccgc gccgagttcc
     8941 agcagcgcgg cgacgcgccc ctcgacctgc acgtcgccat gcgtcggttc gagcgtgccg
     9001 gtgatgatct gcaacagcgt ggacttgccg ctcccgttcg ccccgacgat ccctacggtc
     9061 gtgccgcgct cgatctcgaa actgatctct ctgagcgccc agaactcgcg gtgtcgcttc
     9121 agccacccgc gcgtcagcgt ctctttcaag cggtcgctcg ggtgatcgta gatgcgatac
     9181 tgcttcgcca cgccctcgac gcgcaatgcc gtgctcataa ctgcaaaccc taatgcaaac
     9241 agtggcgagt gacaagtgtg aaaagcagtg atgagcgaca ggtgacaagt gacgagataa
     9301 agccgtgaat cgtgaatcgt cacttgtcac tcgtctctgt acttcccatt gtttacaatg
     9361 cggccatgat ttcggcgcag gattcgcggc ggttggtgtg gcggcctctg tgggagttgc
     9421 cggggcgtgc ggagttgatt cgcgcgctgg tgcggcgcga gttggcggcg cgttatcgcg
     9481 gctctgcgct cggcgtgctg tgggcgatgc tgacgcccgc cgtgatgatc tcgatctata
     9541 cctttatctt cgcgggaatt ttcggcgcgc gtttcggcga gcgcgggacg gcgtgggatt
     9601 acgcgctcta cctcttttgc gcgctcctgc cctggacggc gtttcaggat tccctacagg
     9661 tttcttccaa cgtcatcgtc gcgcacgcga atctcgtcaa gcgcgtggtc ttcccgctcg
     9721 aagccctgcc ggtggcgcag gtgatggccg cgctcaccac gcagatgctc ggcacgatag
     9781 tgttgctcct ggcgacgctc atcatccagc gcgaactgca tctcacactg ctttggctgc
     9841 ccgtcgtcgt cctgccgcaa ctcgtcctga tgctcggcgc ggcatggctc gtcgcttcgc
     9901 tcggagtctt cctgcgcgac acggggcagg tcgtctcgct gctcgtcgtc gcgtggatgt
     9961 ttctcacgcc gatcatctat cccgaacaga tcgtcccggt gcgctatcaa gggttcatca
    10021 acgccaaccc gttcacgccg ctcgtcagaa actatcgccg catcatcctc gaaggcgtcg
    10081 cgcccgactg gcgcggactc gcctacttca cactcttcgc tctcgtcgtc ttcatactcg
    10141 gctactggtg gttcgccaag acgcgcaaga atttcgcaga cgtgatctga aagagccgtc
    10201 agccgtcatc tgttcatctg tcatctgtca tctgtgagcg ggaagcggcg caaggttggg
    10261 ggagcggctt tgttgcctgt cgccgcccgc gaggctttta gggtctgctg ccggatgctt
    10321 tttgcgtgtc gccagaaatc ttgacgactg ttcccatgag ccaccagagg acgagcgcga
    10381 cttccgcgtc gccgaagttg tagttgacga gggaactggc gcagaagccc gcgaacgcgc
    10441 cggttgcgcc gagcagcagt ccgtgtgtgg aggcgtcggc ggatgcgcgc catgcgcgtt
    10501 cgccgcgtgt cagggtgagc cagaagacgg cgagcagcca gagccagagg aggagcgcgg
    10561 gcagtccgcg ctcgaaggcg agttggagcg gcgtcgagtg cgtgtggatc tgcacctcgc
    10621 cgggaaagcc ccattcagtc cagtgtcgtt tcactgcgtc catgccgtgc ccgaagaggg
    10681 gatgctccgg cgtgcgcgtg agggcgacgc gcgcgaccgc cagacgcagg cgcgaactct
    10741 catcttgcaa cctgagcgcg ccttccgcac gcgtccgcca cacggcgaac gcgccgagcg
    10801 cgaccgcgaa cgcgacgata accgtgatga cgaaacgtgt gcgcacgcga gatgtcgcgc
    10861 gccatgcgac gacgcacgcg ccgatggcga aggcgacgag cgcggtgcgc atcgccgtga
    10921 gcgcgatgcc tgccgcgagg cagagaaagg ccgctgcgaa gagtgcgaaa cgaaggcgcg
    10981 gcagcgggca agtgcggcgg cgttgccagt gggcgagcgc gaagccgagc gagagttggg
    11041 cgaggatttg cagcgtctcg gcgaaggtct ggtaatggcg cgtccagccg gaagcgcgga
    11101 agcggtgcga gcgtcgtgtg ccgcgcaggc cggaaggcga cgggcgcgct ttcaaatcgc
    11161 cggacacgcg ctccgtcccg acgcgctccg cctgttcgcc ctgcgaaaat agactcagac
    11221 ggagcggctc gcccacgggc gcgtccctga tcgcctgatc gatctcggcg accgtgttga
    11281 cgcgccgttc gttcacgcgc cagagcgtgt cgcccgccgc gacgccgagc gagggcgagc
    11341 gcagggggct gtccgcagct atttcgtcta tgacgagtcc ccgcccgcgc gccacttcga
    11401 acgcgctcca gagcgcgccc gccacgcccg atgcgatcat cagcgcggcg cagacggcgg
    11461 ccatgcggcg cgtgatgagc gactgtacga aatagaggat gagaaaggtg ctgaccgaga
    11521 tgagcttcag gaggctgatg cgcggttcgg cggagaagag cgcggagagg atgctccacg
    11581 cgtagaagag ccagagcggc agatcgagag cggtgcggcg gaggtttgtg cggcgcgtga
    11641 agagtgtgcg cgcgagccag ccggagatgg agacggagag cgcgatccac gctccggcga
    11701 ttgaatgcgg ggcgaagacg gcgtagagca ggaggccgcc gaacgccgtg gcttcggcga
    11761 gtcggag
//
LOCUS       DASWNS010000433         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_456830, whole genome shotgun sequence.
ACCESSION   DASWNS010000433 DASWNS010000000
VERSION     DASWNS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456830"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VGB76_15030"
     CDS             <1..638
                     /locus_tag="VGB76_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100262.1"
                     /translation="HPKLTPNLVKMLLMYTAQPLSGFNMFEQGAGQLNIEGAVRLAKL
                     VRMDLSPLTFTGSPLLTTNTPPAPRDEIAGQSINWSQGIILNRTYATGLDLINRYQKV
                     YGTGTLLGDGVIEELNAQSVDTTKLSGAVALGENILTSGSGGAWLEDGMGFFSTGMLL
                     GDGIIIGDGIVIGDGIIIGDGIVIGDGIVIGDSRLQAHSVRTNGDDTEAMR"
     gene            887..>2353
                     /locus_tag="VGB76_15035"
     CDS             887..>2353
                     /locus_tag="VGB76_15035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY0100263.1"
                     /translation="MKYSQRFPFIKQFIFSLLLCALLTSELPVTHAAPAHASLRDEQD
                     VERRGARGRSEEKISSELLDASRRGSRKHQNERVKVILQLNGKANPAFELFLQAYGAR
                     TTRNFAKLNARAVELPARAIKALAARGELRFASSDQEMIPAGHVSETTGADAVRNSSS
                     AGALNLPGGTSTYLDGTGIGIAVLDSGIDLGHRAFLNHSNRSRVVLSRDFTGENRIDD
                     PYGHGTHVASIAAGNGRISNAEYIGIAPNANLVNLRVLNSKGQGTTSGLLAALDWVLA
                     NRTLYNIRVANLSLGAPAIESYRNDPVCRAVRRLVDAGIVVVAAAGNNGKNSSGQKIY
                     GQIHSPGNDPSALTVGAANTFGSHPRHDDGVASYSSRGPTRSFWTDALGTKRYDSLIK
                     PDIVAPGNKLVYAQADDNLLVRQNPELDAGISPVDNRKMMYMNGSSMATPVAAGAAAL
                     LLQTHPKLTPNLVKMLLMYTAQPLSGFNMFEQGAGQLNI"
BASE COUNT          487 a          763 c          673 g          430 t
ORIGIN      
        1 ctcatccgaa attaacgccc aacctcgtca agatgctcct gatgtacacg gcgcagccgc
       61 tctccggctt caacatgttc gagcagggcg caggccaact caacattgaa ggcgccgtca
      121 ggctcgccaa actcgtgcgc atggacttgt cgccgctcac cttcaccggc tccccgctcc
      181 tgacgacgaa cacgccgccc gcgccgcgcg acgagatcgc gggtcagagc atcaactggt
      241 cgcagggcat cattctcaat cgcacttacg cgacggggct tgatctcatc aacaggtatc
      301 aaaaagttta tggcacgggc acgctcctcg gcgacggcgt gatcgaggaa ttaaacgcgc
      361 agtcggttga tacgacgaag ctctcgggcg cggtcgcgct cggcgaaaac attttgacga
      421 gcggcagcgg cggcgcatgg ctggaagacg gaatgggctt tttcagcaca ggaatgctgc
      481 tcggagacgg gatcatcatc ggcgacggga tcgtgatcgg cgatgggatc atcatcggcg
      541 acggcatcgt gatcggcgac gggatcgtga tcggcgactc gcgcctgcaa gcacattcgg
      601 ttaggacgaa cggcgacgac acagaggcga tgaggtagaa atctccgtct gcggttgacg
      661 atggctcgtc aacttgaaat actttcgcgt cgtcgctttt ctcccaacct ttgtcaattc
      721 cacctcgcaa gtcgctcagg cgcaactctc tttacgacgt tgcgcgcgga gctatctctg
      781 accatgctcg atgggttcga gttcgatcca gattgagtac ggcacaggcg gcgtctcccg
      841 aagacagttc gatgccgcct cgtgtttagc tcaaaggagg agtccggtga aatactcgca
      901 acgttttccc ttcatcaagc aattcatatt ctcgctgctg ctctgtgcgc tgttaaccag
      961 tgaattgccc gttacgcacg ctgcccccgc gcatgcgagt ctgcgcgatg agcaggatgt
     1021 cgagcgacgc ggtgcgcgcg ggcgcagcga ggagaaaatc tcttccgaat tgcttgacgc
     1081 gtcgcgtcgc ggctcgcgca aacatcaaaa cgagcgcgtg aaagtgatcc tgcaactgaa
     1141 cggcaaggcc aatcccgcct tcgaactctt cctgcaagct tacggcgcac gcacgacgcg
     1201 caactttgcg aaactcaatg cccgcgcggt tgaactgccc gcacgcgcca tcaaggctct
     1261 cgccgcacgc ggcgaacttc gtttcgcctc ttccgatcag gagatgatcc cggcaggcca
     1321 cgtctcggaa acgacgggcg cggacgccgt gcgcaactcg tcgagcgcag gcgcgcttaa
     1381 tttgccgggc ggaacctcca cctatctgga cggaacgggc atcggcatcg ccgtgctcga
     1441 ttcgggcatc gacctcggcc accgcgcctt cctgaatcac agcaacaggt cgcgcgtcgt
     1501 cttaagccgc gacttcacgg gcgagaaccg cattgacgat ccttacggtc acggcacgca
     1561 cgttgcctcc atcgccgccg gaaacgggcg catctcgaac gccgaatata tcggcatcgc
     1621 accgaacgcc aacctcgtca acctgcgcgt gctcaactcg aaaggacagg gcacgacctc
     1681 cggcctgctc gccgcgctcg actgggtgct ggcaaatcgc acgctctaca acatacgcgt
     1741 cgccaacctg agcctcggcg cgcccgccat cgaatcctat cgcaacgatc ccgtgtgtcg
     1801 cgccgtgcgt cgtctggttg acgccggaat agtggtggtc gcggcggcgg gcaacaacgg
     1861 gaagaacagt tcggggcaaa aaatttacgg gcagattcat tcgccgggca acgacccttc
     1921 cgcgctcacg gtcggcgcag ccaacacttt cggctcgcac ccgcgccacg acgacggcgt
     1981 cgccagctac agttcgcgtg gcccgacgcg aagcttctgg actgacgcgc tcggcacgaa
     2041 acgctacgac agtttgatca agccggacat cgtcgcgccc ggcaacaagc tggtctacgc
     2101 acaggccgac gacaatcttc tcgtgcggca gaacccggaa ctcgatgcgg gcatcagccc
     2161 cgtcgataac cgcaaaatga tgtacatgaa tggctcttcg atggcgacgc ctgtggccgc
     2221 cggagccgcc gccctgctgc tgcaaactca tccgaaatta acgcccaacc tcgtcaagat
     2281 gctcctgatg tacacggcgc agccgctctc cggcttcaac atgttcgagc agggcgcagg
     2341 ccaactcaac att
//
LOCUS       DASWNS010000434         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_171030, whole genome shotgun sequence.
ACCESSION   DASWNS010000434 DASWNS010000000
VERSION     DASWNS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171030"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1149
                     /locus_tag="VGB76_15040"
     CDS             25..1149
                     /locus_tag="VGB76_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY0100264.1"
                     /translation="MARSAEIGEKVGRVERMLAAEGLGGVLLGAQHNFSWLTAGGSNG
                     IDLSRDAGAGALLVRADGKRFVLASRIEMPRLLAEELAGEEFEPVEFAWEEEKASPTF
                     LADRARSLLEGTSALGSDLPVSPEVRVIEGALAGCRYRLTPPEIERFRQLGQDAGRAS
                     SDLVRSLEPGETEQEVARRAQDALAAGGMRAVVCLVAADERIRQFRHPVPTARRWEKV
                     LMLVVCARRGGLIASLTRIICAGAALEDLQRRTEAVARVNASLLALTRPGATGAELYR
                     TAARAYDAAGFTGEARLHHQGGAAGYRTRDWVAHPASAEVVQDGQAFAWNPSITGTKA
                     EETIIAREDGVEVVTRTSDWPQVAVEVDGSEYLSPGVLLV"
     gene            1200..2591
                     /locus_tag="VGB76_15045"
     CDS             1200..2591
                     /locus_tag="VGB76_15045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0100265.1"
                     /translation="MSDTSKVVQATALPKSKSEVAPPQAAGASVVGAAIAASEGVGGR
                     IGRYRWVICALLFFATTINYIDRQVLGILATDENFKRTIGWNEAEYGYVNTAFQAAYA
                     IGLLVVGNLMDRFGTRKGFSFAIVWWSIAAMGHALARSPVGFGVARFALGLGEAGNFP
                     AAIKTVAEWFPKKERALATGIFNSGSNVGAIIAPLSVPWIAVTYGWQWAFIITGAIGF
                     IWLAFWLAAYRRPEEHPKLSAAELAHIQSDPAEPTTKIPWARLFPHRQTWAFAFGKFL
                     TDPIWWVYLFWLPKFLNTNYGLNITQIGLPLVVIYVAADFGSIGGGWLSSSLIKRGWT
                     VNKARKTAMLLCAVSVVPIMFAANASNLWVAVGLISLAAAAHQGWSANMFTTASDMFP
                     RRAVGSVVGIGGMAGSIGGMLIATTVGLILQYTGSYLPIFIIAGSAYLVALLVIHLLA
                     PRLEPANVEAPAR"
BASE COUNT          445 a          821 c          936 g          493 t
ORIGIN      
        1 ctcgtcactc gtcactgttt tattatggct aggagcgcgg aaatcgggga gaaggtaggc
       61 cgggtggagc gtatgctggc ggctgagggg ttgggcggcg tgctgttggg cgcgcagcac
      121 aatttctcct ggcttacggc gggcggttca aacggcatcg acctgagccg tgatgcgggc
      181 gcgggtgcgc tgctcgtgcg cgcggacggg aagcgtttcg tgctcgccag ccggatcgag
      241 atgccgcgac tgctggcgga agagttggcg ggggaagagt ttgaacccgt ggagttcgcg
      301 tgggaagagg agaaagcctc cccgacgttt ttggccgacc gcgcgcgttc gctgttggaa
      361 ggcacctccg ctctcggctc cgacctgccc gtgagtcctg aagtccgcgt catcgaaggc
      421 gcgctcgccg gttgtcgcta caggctcacg cccccggaga tagagagatt caggcaactg
      481 gggcaggacg cggggcgcgc gtcgagcgat ctggtcagga gcctcgaacc gggcgagacg
      541 gagcaggaag tggcgcgacg cgctcaggat gctctcgccg caggggggat gcgcgccgtc
      601 gtgtgtctgg tcgccgcaga cgagcggata cggcagtttc gccatccggt gccgacggca
      661 cgccgttggg agaaggtttt gatgttggtg gtctgcgcac gtcgcggcgg actaatcgcg
      721 agcctgacgc gcatcatctg cgcgggcgct gcccttgaag atttacaaag acgaaccgag
      781 gcggtcgcgc gcgtcaacgc gagcctgctc gcgttgacga ggccgggcgc gacgggcgcg
      841 gagttgtacc ggacggctgc gcgcgcctac gacgccgcag ggttcacagg cgaggcgcgg
      901 ctgcatcatc agggcggcgc agccggttac cgcacacgcg attgggtggc acatccggcg
      961 tcggcggaag tggttcagga cgggcaggcg ttcgcctgga atccatccat cacgggcacg
     1021 aaggccgagg aaaccatcat cgcccgcgag gacggggtgg aagtcgtcac gaggacatcg
     1081 gactggccgc aggtcgccgt cgaagttgac ggcagtgagt acctttctcc gggcgtgctt
     1141 ctcgtctgac gagcgcgccc gtaccgttca aacccatttc aaaaagacgg ggtaataaga
     1201 tgagcgatac gagtaaggtg gtgcaggcga ctgcgctccc gaaatcaaag tcggaggttg
     1261 cgcctccgca ggcggcgggc gcgagtgtgg taggcgcagc catcgcggcg agcgaagggg
     1321 tcggcgggcg tattggccgc taccgttggg tcatctgcgc gctgctgttc ttcgcaacga
     1381 cgatcaacta catcgaccgg caggtgctcg gcatcctcgc caccgacgag aatttcaaac
     1441 gcaccatcgg ttggaacgag gccgagtacg gctacgtcaa cacggcgttt caagccgcct
     1501 acgccatcgg gctgctggtg gtcggcaacc tgatggacag gttcgggacg cgcaagggct
     1561 tctcgttcgc catcgtctgg tggtcgattg cggcgatggg gcacgctctg gcgcgttcgc
     1621 ccgtcggctt cggcgtggcg cgcttcgcgc tcgggttggg cgaggcgggc aacttccccg
     1681 cggccatcaa gacggtggcc gagtggtttc cgaagaagga gcgcgcgctc gccacgggca
     1741 tcttcaactc cggctcgaac gtgggtgcca tcatcgcgcc gctctccgtt ccgtggattg
     1801 cggtcacgta tggctggcag tgggcgttta tcatcacggg cgcgataggc ttcatctggc
     1861 tggccttctg gctcgccgcc taccgccgcc ccgaagagca cccgaagctc tccgcagccg
     1921 aactggcgca catccagtca gacccggcgg agccgacgac gaagattccc tgggcgcgac
     1981 tcttcccgca ccggcagacc tgggctttcg ccttcggcaa gtttctcaca gacccgatct
     2041 ggtgggtcta cctcttctgg ctgccgaagt ttctcaacac caactacggc ctgaacatca
     2101 cgcagatcgg cctcccgctc gtcgtcatct acgtcgccgc agacttcggc tcaatcggcg
     2161 gcggctggct ctcctcgtcg ctcatcaagc gcgggtggac tgtgaacaag gcgcgcaaga
     2221 cggcgatgct gctctgcgcc gtgtcggtcg tgcccatcat gtttgcggcg aacgcctcga
     2281 acctgtgggt cgcggtcggg ctgatcagtc tggcggcggc ggcacatcag ggctggagcg
     2341 cgaacatgtt cacgacggcc tcggacatgt ttccgcgccg cgccgtcggc tcggtcgtcg
     2401 gcatcggcgg catggccggt tccatcggcg gcatgttaat cgccaccacg gtcggcctta
     2461 ttcttcaata cacaggctcg tacctgccca tcttcattat cgccgggtcg gcctatctcg
     2521 tcgcgctgct ggtcatacac ctgctcgcgc cgcgcctcga accggctaac gtggaggctc
     2581 ccgcgagata ggtggagact atgagcaagc tactcgacgt gaaaccgaaa gagggcagcc
     2641 gttgggattt ggtgagcctc ggcgaagtga tgttgaggct cgaccccggc gacgc
//
LOCUS       DASWNS010000435         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_94809, whole genome shotgun sequence.
ACCESSION   DASWNS010000435 DASWNS010000000
VERSION     DASWNS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94809"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /gene="murD"
                     /locus_tag="VGB76_15050"
     CDS             complement(<1..949)
                     /gene="murD"
                     /locus_tag="VGB76_15050"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY0100266.1"
                     /translation="MQVEGKKVLIIGAARSGIAAARFLAARGATVALNDRKAIEEWTD
                     EARALKAEGVGLLAGDVPMWLMDQIELVVVSPGVPSKNIPVRYMERAGAEVIGEVELA
                     ARFLRGRIVAITGTNGKTTTTALVTELLRGADLPVQVGGNIGTPLVSLVESSREDGWT
                     VAEVSSYQLETIADFHPTVAVVLNLMPDHMDRYETLTDYGAAKHRIFRNQAAGDVAIL
                     NADDDIVASWASGLRAHVVLFSVERELEEGLFLRDGRELVSRTREGERVLLARDEMQL
                     RGLHNVQNVLAALACGLACGASPDAMRETVRNFRPVEHRL"
     gene            complement(976..>1606)
                     /gene="mraY"
                     /locus_tag="VGB76_15055"
     CDS             complement(976..>1606)
                     /gene="mraY"
                     /locus_tag="VGB76_15055"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY0100267.1"
                     /translation="PFFKQTDITRITNIGPWLYLPFILVVLLGSSNAVNLTDGLDGLA
                     ISVTFIAMVALTAFTYVSGDKRWADILFIEHRREVGELTVFCGAMAGASLGFLWYNAP
                     PADVFMGDVGSLAIGGAIGTIGILTKQEFILVLVGGVFVLEAISVMLQVSFFKMTKRH
                     TGTGRRLFKMTPLHHHFEMQGWKESKVVFRFLILAILFALLSLSTLKLR"
BASE COUNT          328 a          545 c          448 g          285 t
ORIGIN      
        1 ccagacgatg ctcgaccggg cgaaagttcc tcaccgtctc gcgcatcgca tcgggcgacg
       61 cgccgcacgc cagcccgcac gcgagcgcgg ccaaaacgtt ctgcacgtta tgcaggccgc
      121 gcaactgcat ctcatcgcgc gcgagcagca cccgctcgcc ttcgcgcgta cgcgaaacga
      181 gttcgcgccc gtcgcgcaaa aacagtccct cttccagttc cctctcgacg ctgaacagaa
      241 cgacgtgcgc gcgcaagccc gacgcccacg aagccacaat gtcgtcgtcg gcgttcagga
      301 tcgccacgtc gcccgccgcc tgattgcgaa agatgcgatg cttggccgcg ccgtaatcgg
      361 tcagagtctc gtaacgatcc atgtggtcgg gcatcaggtt caacacgacg gccacggtcg
      421 ggtgaaagtc tgcaatcgtc tcaagctggt aactcgaaac ttccgccacc gtccaaccgt
      481 cttcgcgcga cgattcgact aaagagacga gcggtgtgcc gatgttgccg ccgacctgca
      541 cgggcaagtc cgcgccgcgc aacaactccg tgacgagcgc ggtcgtcgtc gtctttccgt
      601 tcgtgcccgt gatggcgacg atgcgcccgc gcaggaagcg cgccgcgagt tccacttcgc
      661 cgatcacctc cgcgcccgcg cgctccatgt aacgcaccgg gatgtttttc gaaggcacgc
      721 ccggactcac caccacgagt tcgatctgat ccatcaacca catcggcacg tcgcccgcca
      781 gcaaccccac gccttccgcc ttcaacgccc gcgcctcatc cgtccactct tctatcgctt
      841 tgcgatcatt gagagccacg gttgccccgc gcgccgccaa gaaccgcgcc gccgcaatgc
      901 cgctgcgcgc cgcaccgatg atcagaactt ttttcccttc aacctgcaaa tttttcagct
      961 ttcaattatc aacatctaac gcagtttcaa agtcgagaga ctaaggagcg cgaacaggat
     1021 cgcgaggatc aaaaatcgaa agacgacttt cgactccttc cagccctgca tctcgaagtg
     1081 gtgatggagc ggcgtcatct tgaagagtcg ccgcccggtg cccgtgtgcc gcttcgtcat
     1141 cttgaagaaa gagacttgca gcatcaccga gatcgcttcg aggacgaaca cgccgccgac
     1201 gagtacgagg atgaactcct gtttcgtcaa aatcccgatg gtgccgattg cgccgccgat
     1261 ggcgaggctg cccacgtcgc ccatgaacac gtctgcgggc ggcgcgttgt accacaaaaa
     1321 gccgaggctc gcgcccgcca tcgcgccgca aaagaccgtt agttcgccca cttcgcggcg
     1381 atgctcgatg aacagaatat ccgcccaacg cttgtcgccg ctgacatagg tgaaggccgt
     1441 cagcgcaacc atcgcgatga aggtgacgct gatggcgagt ccgtcgaggc cgtccgtcag
     1501 gttcaccgcg ttcgacgagc cgagcagcac gacgaggatg aagggcaggt aaagccaggg
     1561 tccgatgttc gtgatgcgcg tgatgtccgt ctgcttgaag aacggg
//
LOCUS       DASWNS010000436         5096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_16974, whole genome shotgun sequence.
ACCESSION   DASWNS010000436 DASWNS010000000
VERSION     DASWNS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16974"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2609)
                     /locus_tag="VGB76_15060"
     CDS             complement(<1..2609)
                     /locus_tag="VGB76_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEY0100268.1"
                     /translation="MSNTSDRVSQLSPVKRALLAVEQMQSQLAAAERARTEPIAIVGM
                     ACRFPGGANSPAAFWRLLRDGVDAVTQVPAERWDIEEYFDADPAAPGKMYTRWGAFIE
                     DVYKFDASFFGISPREALSIDPQHRLLLEVSWEALEYAGRSQDVTGSKTGVFVGLTNA
                     EYFQQVKLGGTAGIDAYYGTGNVLSSAAGRLSYAFGLQGPCMTVDTACSSSLVTTHLA
                     CQSLRNRECDMALAGGANLILLPDVNIYMSKARTMAADGRCKTFDASADGYVRGEGCG
                     IVVLKRLSDAVADGDNILALIRGSAVKHDGRSGGYTVPNGGMQQELIREALANAGVEP
                     SAISYIEAHGTGTSLGDPIEMRALGAVLKDEGRPQKLAVGSVKTNIGHLESAAGIAGL
                     IKTVLALQQCEIPPHLHLKEVNPQISLEELGFTIPTTSLPWPLDPARKRFASVSSFGL
                     TGTIAHVVLEEASGNVESVAAARAQDADAGAEAQLLTLSARDPHALEALAELYRAYLD
                     GEGAQESLADICYTAGTRRAHHEHRLALTGRTHAELIERLEAFGRGELFPGVEARRKQ
                     ATQSPKLVFVFPGQGSQWAGMGRQLLRTEESFRASMERCDALMREHQGWSLLAELTAD
                     AEHSRLDEIDVVQPALFAMQVSLAALWRSWGIEPAAVIGQSMGEIAAAHVAGALSLED
                     AVKIICRRSQLLKSRSGMGSMAAVELTLEHTQEALKGYADRLSVAVSNSPTSTVIAGE
                     TAALEELLESLRGRFVSCRQVKVNIASHSPQMEPLREPLLRSLAEVRPQSLALPFYST
                     VTGEFDRNLNLDAAYWWRNLREPVLFSGTVQRLLEGGYVSFLEVSPHPIVLTSIQQGM
                     YHLDQEGTVLPSLR"
     gene            complement(2614..>5096)
                     /locus_tag="VGB76_15065"
     CDS             complement(2614..>5096)
                     /locus_tag="VGB76_15065"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0034081 - polyketide synthase complex
                     [Evidence IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEY0100269.1"
                     /translation="FRGVQQVWQRDGEAIARIALDGAVSEDTAPAGGVHPLLLDNCLQ
                     VIACALPLQAGQAEGVTYLPVGVGRVRVGRRHIPAGVAWSYATLQTPLEDLPPVLTAD
                     AMLLTEAGEVVLELRGVRLQRVRSSVRPEAARGARAADESRRAAGEDTGAWLYEIEWR
                     REELPSTQTPASTQVEAQGKWLIFADDSGVGEAVRSRLEVEEADACVLVRVGSSYRRV
                     APNEFEVDPASARDFDQLLAEASDFGLPGWRGVLHLWGLEEATAQAQLTANDLAQAQT
                     VGCRSILHLLQSLDRAGLARSVRLWLATTGAQGPAGARLPSGIAHAPVWGLGRVIARE
                     FHSLRCTMIDVNNAAPEAKAAALFQELRSFSGENQVAWSETARYVARLQRWKGNSVAP
                     ETDGTRKGVQPSPESPFSLDAEGTYLITGGLGGLGLKLAGWMVARGARHLALVGRSGA
                     TAPAREVVAQLEAAGAQVRVIAADVAAREQVEQALAEIAATMPPLKGVVHAAGVLEDA
                     LLGQQDQERFARVLAPKLQGAWNLHSLTVGAKLDFFVLFSSISSVLGSMGQSGYAAGN
                     SFMDALAHHRRALGLHGLSINWGPWAEVGMAASHAKDFARQGIDSIRPEAGFRVLERL
                     LVEGATQVGVLPVRWTEFIASLPSPQREPFLSDLFDTASASPEQSVERPARSGSTQAT
                     PPLVVQLTEALPSERLAILTARIQAEACGVLGLPSSAQLDVAQGFFDLGMDSLMIAEL
                     ARNLEAAVGRPFPLSMMFEHANVDALAHYLSEHVLDLKLNNETPRPAAVKEETAEAEL
                     EQLEHLEHLEQLSDEEALALLTNKLSLE"
BASE COUNT          917 a         1722 c         1559 g          898 t
ORIGIN      
        1 cgcagcgacg gcagcacagt cccctcctga tcgaggtgat acatgccctg ctgaatcgag
       61 gtcaggacga ttggatgcgg gctgacttcg aggaagctca cataaccgcc ctccagcaga
      121 cgctgcacgg tgccggaaaa gagaaccggc tcccgcagat ttcgccacca gtaagcggca
      181 tccagattga ggttgcggtc aaactctccg gtcacggtgg agtagaacgg caacgctaaa
      241 gactgcgggc gcacctccgc tagacttcgc agcagcggct cgcgcagagg ctccatctgc
      301 ggactgtgcg aggcgatgtt gaccttcacc tgacgacagg agacgaagcg cccgcgcagg
      361 ctctccagca actcttcgag cgcggctgtc tctccggcga tgacggttga ggtcgggctg
      421 ttgctgacgg cgaccgatag cctgtcggca tagcctttca acgcctcctg cgtgtgttcg
      481 agagtcaact cgaccgccgc catgctgccc atcccgctgc gacttttcag caattggctg
      541 cggcggcaga taatcttcac ggcgtcttcc agactcaagg ctcccgccac gtgcgccgcc
      601 gcgatctcgc ccatgctctg cccgatgaca gccgccggtt cgatgcccca cgaacgccag
      661 agcgcggcga gcgagacttg catggcgaac agcgcgggct gcaccacgtc tatctcgtcg
      721 agccttgagt gctccgcgtc ggcggtgagt tctgccagca aagaccagcc ctgatgctcg
      781 cgcatcaacg cgtcgcaacg ctccatactc gcgcggaaag attcttccgt gcgcagcagt
      841 tgacgcccca tcccagccca ttgcgacccc tgaccgggga agacgaagac cagcttcggc
      901 gattgggtgg cctgcttccg ccgcgcttcg acgccgggga acaactcgcc gcgtccaaat
      961 gcttcgaggc gttcgatcag ttcggcgtgt gtgcgtcccg tcaaagccag acggtgctcg
     1021 tgatgcgcgc gacgtgtgcc agccgtgtag caaatgtccg cgagagattc ctgcgcgcct
     1081 tcgccgtcga gataggcgcg gtaaagctcc gccagagcct caagcgcgtg cgggtcgcgg
     1141 gcggagagcg taagcaactg tgcctctgcc ccggcgtctg catcctgcgc gcgggcggcg
     1201 gcgactgatt cgacgttgcc cgacgcctct tccagtacaa cgtgtgcgat tgtgccggtg
     1261 aggccgaacg aactcacgct ggcgaaacgc ttccgcgccg ggtcgagcgg ccagggaagg
     1321 ctcgtggtcg ggatggtaaa gcccaactct tcgagcgaaa tctgcgggtt gacctctttc
     1381 aggtggaggt gcggcggaat ctcgcactgt tgcagggcga gcaccgtttt gatgagtccg
     1441 gcgatgcccg ccgccgattc gaggtgtccg atgttggtct tgacggagcc gacggcgagt
     1501 ttctgcggtc gcccttcgtc cttcaagacc gcgccgagcg cgcgcatctc gatggggtcg
     1561 cccagagagg tgccggtacc gtgagcctcg atatagctga tcgcagacgg ctcgacgccc
     1621 gcgttagcca aagcctcgcg gatcaattcc tgctgcatgc ctccgttggg gacggtatag
     1681 ccgccgctcc gcccgtcatg cttgaccgcc gagccgcgaa tcagggcgag gatgttatcg
     1741 ccgtcggcga ccgcatcgga gagtcgcttg agcacgacga tgccgcagcc ttcgccgcgc
     1801 acgtagccat cggctgaagc gtcgaaggtc ttgcagcgac cgtcggcggc catcgtccgg
     1861 gctttgctca tgtagatgtt gacatcgggc aggagaatca gattcgcgcc gcccgccagc
     1921 gccatgtcac actcgcggtt gcgcaggctt tgacaggcga ggtgggttgt caccagcgac
     1981 gacgagcagg ccgtgtccac ggtcatgcac ggcccttgca gcccgaacgc gtaagagagg
     2041 cgacccgcag ccgaactcaa cacgttgccc gttccgtagt aggcgtcgat gcccgcagta
     2101 cctccgagct taacctgctg gaagtattcg gcgttcgtca gcccgacgaa aacgcccgtc
     2161 ttgctccccg tcacatcctg actgcggcct gcgtactcca gagcctccca ggagacttcg
     2221 agcaacaggc ggtgttgcgg atcgatgctg agcgcctcgc gcggggagat gccgaagaag
     2281 ctggcgtcga acttgtacac gtcttcgatg aaggctcccc aacgcgtgta catcttgccg
     2341 ggtgctgcgg ggtctgcgtc gaagtattcc tcgatgtccc aacgttcggc gggaacttgc
     2401 gtaacggcgt ctactccgtc gcgcagcaac cgccagaagg ccgcagggct gtttgcgccg
     2461 ccggggaacc ggcaggccat gccgacgatg gcaatcggct ccgtccgcgc gcgctcggcg
     2521 gcggcgagtt gtgactgcat ctgctcgacg gcgagcaggg cgcgtttgac gggtgaaagc
     2581 tgagagacgc ggtctgatgt gttgctcatg ttgctattcc agtgaaagtt tatttgtcag
     2641 taaggccagc gcttcttcat ccgagagctg ttcgaggtgt tcgaggtgtt cgagctgttc
     2701 gagttccgcc tccgccgtct cctccttcac ggctgcgggt cggggcgttt cgttgttgag
     2761 cttcaaatcc agaacgtgtt cggacaggta gtgtgccagc gcatcgacgt tcgcgtgctc
     2821 gaacatcatg ctcagaggga acggccttcc cacggcggct tccagattgc gcgccagttc
     2881 cgcaatcatc agcgagtcca tgccaagatc gaagaagcct tgcgcgacat cgagttgcgc
     2941 ggaggagggc agccccaaca ccccgcaagc ctcggcctga atgcgtgcgg tgagaatggc
     3001 aaggcgctcg gacggcaacg cctccgtcaa ctgtacgacg agcggcggcg tcgcctgagt
     3061 cgagcctgac cgggcgggac gctcgacgga ctgctccggc gatgcggaag ccgtgtcaaa
     3121 gagatcgctg aggaacggtt cgcgctgcgg cgacggcaag gaggcgatga actccgtcca
     3181 ccggacaggc aacacgccca cctgtgtagc cccttcgacc agcaagcgtt cgagcacgcg
     3241 aaagcccgct tcaggccgga tgctgtcgat gccttgccgc gcaaaatcct tagcgtgcga
     3301 ggcggccatg cccacctcag cccacggccc ccagttgata ctcaagccat gcagaccgag
     3361 cgcgcgccgg tggtgcgcca gtgcatccat aaacgagttg cccgccgcgt aaccggactg
     3421 acccatactg ccgagcacgg acgaaatgga agagaacagc acgaagaaat cgagctttgc
     3481 accaaccgtc aagctgtgta gattccaggc tccctgaagc ttcggagcca gcacgcgcgc
     3541 aaaacgctcc tgatcctgtt gacccaggag cgcatcctcc agcaccccgg cggcatgcac
     3601 cacgcctttg agcgggggca ttgtcgccgc aatctcagcc aacgcctgct cgacctgttc
     3661 ccgcgccgcc acgtcggcgg cgatcacgcg cacctgcgcg cccgccgctt ccagttgtgc
     3721 aacgacttca cgcgccggag ccgtcgcgcc gctgcgcccg accaacgcca gatgccttgc
     3781 cccgcgcgcc accatccagc ccgcgagttt cagtcctaaa cctccaagcc cgcccgtaat
     3841 caggtacgtg ccctccgcat cgagcgaaaa cgggctttcc ggcgaaggct gaactccctt
     3901 gcgcgtgcca tctgtttccg gcgcaacaga gtttcccttc cacctctgca atctggcgac
     3961 gtaacgcgcc gtctcgctcc acgccacttg attctccccg gagaacgagc gcaactcttg
     4021 aaagagtgcg gcggccttcg cttccggcgc agcattgttg acatcaatca tcgtgcagcg
     4081 cagcgaatgg aactcacgcg cgatcacacg accgagtccc cacacgggcg cgtgtgcgat
     4141 gccggacggc aggcgcgcgc ccgccggtcc ctgcgcacct gttgtcgcta accagaggcg
     4201 aacgctccgc gcgagtccgg cgcggtcgag cgattgcaaa agatgcagga tgctccggca
     4261 gccgacggtc tgcgcctgcg cgagatcatt cgccgtcaac tgcgcctgcg ccgtcgcctc
     4321 ttccagaccc cagaggtgca gcacgccgcg ccagcccggc agtccgaagt ccgaagcctc
     4381 cgccagcaat tgatcgaagt ctctggccga cgccggatca acctcaaact cattcggcgc
     4441 gacgcgtctg tatgaactac cgacccgcac caacacgcag gcgtccgcct cttcaacctc
     4501 cagacgcgac ctgacagctt cgccgactcc gctatcatcc gcgaagatca gccatttgcc
     4561 ctgcgcttca acttgcgtgg aggcaggcgt ctgagttgac ggaagctcct ctcgacgcca
     4621 ctcgatctcg tagagccacg cgccggtatc ctctcccgcc gcgcgccgcg attcatccgc
     4681 cgctcgtgct cctcgcgccg cttcgggtcg aacgctactg cgcacgcgct ggagacgtac
     4741 gccgcgtagt tcgaggacta cttcgcccgc ctccgtgagc agcatcgcgt ctgccgtcag
     4801 caccggcggg aggtcttcca gaggcgtttg caaggtggcg taactccacg ccacgcccgc
     4861 ggggatgtgt cgtcgcccga ccctgactcg tcccacgccg accggcaagt aagtgacgcc
     4921 ttccgcctgc cccgcctgta acggcagagc gcaggcgatc acctgcaagc agttgtcgag
     4981 caatagcgga tgcacgccgc ccgcaggcgc ggtatcctcc gaaaccgccc cgtccagagc
     5041 gatgcgcgcg atggcctcgc cgtcgcgctg ccacacttgc tgtacgcccc gaaagc
//
LOCUS       DASWNS010000437         7323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_432631, whole genome shotgun sequence.
ACCESSION   DASWNS010000437 DASWNS010000000
VERSION     DASWNS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432631"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="VGB76_15070"
     CDS             <1..1731
                     /locus_tag="VGB76_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0100270.1"
                     /translation="NFPALAAAPPDTDVVRAMFEGLTEYDPKTLTPVSGVAIRWESEA
                     EDRVWIFHLRHDARWSNGEPVTAHDFVRSWQRTLELGERAPHAKLLANLEGVPVKASN
                     PAGAPSPAPSPSNDEMNAGGASRVTEPGAGASPAKRELGVEAVNDYTLKVRLQRPDKN
                     FPALVAHPVFRPVYRAESAKESEREAAVEPSGEDGAKEATPESGAAKAAPVISNGAFN
                     LSAQAADGVVLERAESYWNAKIVSLERVRFVAAPDSEAALSAYRAGEVDAVTNTNIEP
                     LALKLLTPYEDFRRATFGALTFYDVNITRAPFSDLRVRQALSLAIDRERLSADTLGGA
                     TEAAAKFLPSTAAKSSENNAGIESSVTFDAERARRLFAEAGFAGGENFPRLRLLVNRN
                     KQHRTVAAAVAAMWRNVLGVETEIMVRDWDEYEAALRAGDYDVARRSMVMQTVDEETN
                     MLAMFAPDRLSAAANADTNGGASTEAQAAPSPRAEPPGGTAAAGGEESQGGASLAVAA
                     QPILSEQQALKELPALPIYFASSYALVKPYVAGFDSNLLDAPSLQHVRIETNWQPPKR
                     DTPIRIVSNR"
     gene            1792..2799
                     /locus_tag="VGB76_15075"
     CDS             1792..2799
                     /locus_tag="VGB76_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0100271.1"
                     /translation="MSHPAPLLSVRNLCTYFGSADGVVKAVDGVSFDLRRGETLGIVG
                     ESGSGKSVTSLSLLRLIPEPPGKILRGEVVFDGQDILSLPLRAVRQIRGKRIGMIFQD
                     PMASLNPFMRVSTQLMELTRLHLRHTKREAHEHAVRMLDTVGIPDARVRAESYPHEFS
                     GGMRQRVMIAMALSCQPELLIADEPTTALDMTIQAQILELVKSLKSKTNLSVILITHD
                     LGVVAGMTDRVIVMYAGKVFEEAPTRELFARPANPYTKALLRSVPDPESAQGALYQIP
                     GHPPDVSRLPAGCPFAPRCERAEEVCRREFPPFVQLTREHSSLCHFAAEVYAQTNERQ
                     A"
     gene            2796..3998
                     /locus_tag="VGB76_15080"
     CDS             2796..3998
                     /locus_tag="VGB76_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0100272.1"
                     /translation="MNAHDDTQTHFADASHTLPADVAAAGGEPLVVVRDLKVHFAQGG
                     GGSLRDKFAGGTLARRGGDRSMSQGESQQEGRGVLKAVDGVSFEIKRGETLGLVGESG
                     CGKSTLGRALLRLAPVTSGRLFFRGQELTNLSSRELRRSRRYMQMIFQDPYASLNPLM
                     TVGQIVSEPIETFRLASGRERAERVRELLETVGLSARHAGRYPREFSGGQRQRIGIAR
                     ALAASPEFIVADEPISALDVSIQAQIMNLLERLRSERKLTYLFISHDLRAIRHVSDRI
                     AVMYLGKLVELGAAGSVYDDPLMPYTKALISAVPFADPVREAARRRIVLAGDVPSPLN
                     PPRGCRFHTRCPYAIQACREVAPALAEIKPAHFAACIRISPEEPSIERVADEAAPGLK
                     NPAQFAGT"
     gene            4279..6375
                     /locus_tag="VGB76_15085"
     CDS             4279..6375
                     /locus_tag="VGB76_15085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0100273.1"
                     /translation="MEKSAATERAKFRNEELPILQEWERWLASVTAETDLAILLVEGH
                     QPPQLAISNNNSICHAFQSSTTHAHLCDPYCGVAFQRAHEAGEATHYRCHAGLHCFAM
                     PVGMDASRPLAVIGGRAFLSSADYRELAERFRHGDLRELLSTELFRNVIFASRHHLDE
                     LAAQLAEASVTADARRATGSSPTNKGAHTETPREQAAVEAKGGELSRPRGVNFPPGLK
                     LLDACQIALEKLRVRFKLKSVVLMLRTGDDFTPACGMGKFKNRSPQLSAKGKSDEHLK
                     LVGGTDASGVAAELFALSDDETEVFPLVVGDELKGALLVADGKLPEEKRRGLSEFCLE
                     VAMPLEVLRLREELERSLRASYHLQAFTQQINEVEPAEAYAAILRHSTELLRSERGSL
                     LLFDEAANELEVKAAVGPRADVTQDERVRVNESVSGSVLLDGRPRIVRDLRTTELHRA
                     PAERKYKSDSFISYPIITGGRKVGVINMTDKAGGEPYNDFDLHLLETMVPQMALALDR
                     AGWHQKATQFQLLSITDPLTGLLNRRYLEERLGEEFERSKRHRFPMSFLMVDIDNFKE
                     YNDRNGHQAGDLALEITAQCLKSALRSADVASRYGGEEFSILLPQTNMAEALVIAERI
                     RSRVEKSSYPHGDSQPHGAVTVSIGVSAFGPKLETPASIIGAADHALYVAKSRGKNCV
                     VSYVLPPNTSPARDVN"
     gene            6365..>7323
                     /locus_tag="VGB76_15090"
     CDS             6365..>7323
                     /locus_tag="VGB76_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100274.1"
                     /translation="MSTENLFKAAPAKRILGRVAPENFIGRSEHLREISALATQLSER
                     RGLLIHAAPAAGASELLRQAYDKLFQQRGGASPIYFAFTRQEQSIEAAARRFLHTFLT
                     QLVAHRRHDSSFVNAPPALRDLVDLADPADYEWIERLIVTYERAREGTDELALVRLCF
                     SAPVLAAARGARTVILIDEIQNIEKLRGEVELGNEIAQVSMHGDVPFVLCGLRRGLLD
                     ELSGGNAPHRFDGFGTIHLEGLKEADARTLVERLAYTERVALNDETRDLIVQLFNGSP
                     FYITSFMYAAHREGVSLTSFLECQKIYVDEVLGGRINRRFNAIL"
BASE COUNT         1406 a         2351 c         2243 g         1323 t
ORIGIN      
        1 aatttccccg cgctcgcagc cgctccgccg gatacagacg tggtgcgcgc catgtttgaa
       61 ggcttgaccg agtatgatcc gaaaactctg acgccagttt cgggcgtggc gattcgctgg
      121 gagtcggagg cggaagatcg cgtgtggatt tttcacctgc gccacgatgc gcgctggtcg
      181 aacggcgagc cggtgacggc gcacgacttt gtgcgctcct ggcaacgcac gctcgaactc
      241 ggcgaacgcg cgccgcacgc gaaactttta gccaacctcg aaggcgtgcc cgtgaaggcg
      301 tcgaatccgg cgggtgcgcc gtcgcccgcg ccttccccgt cgaatgatga gatgaatgct
      361 ggcggggcgt cgagagttac ggaaccgggc gcgggtgcgt cgcccgcgaa gcgtgaactc
      421 ggagtcgagg cggtcaacga ttacactctg aaagtccgcc tgcaaaggcc ggataaaaat
      481 ttccccgcgc tcgtcgcgca tccagtcttt cgtcccgtct accgtgcgga gagcgcgaag
      541 gagagcgagc gggaagcggc ggtggagccg tccggggaag atggcgcgaa ggaggcgacg
      601 cctgagtccg gtgcggcgaa ggctgcgccc gtcatctcaa acggcgcgtt caatttgagc
      661 gcgcaggcgg cggacggcgt agtgctggaa cgcgcggaga gttattggaa cgcgaagatc
      721 gtgtcgctcg aacgcgtccg tttcgtcgcc gcgcctgatt cggaagccgc gctctcggcc
      781 tatcgtgcgg gcgaagtgga cgccgtgacc aacaccaaca tcgaaccgct cgctctgaaa
      841 ctgctcacgc cttacgaaga ttttcgccgc gccactttcg gcgcactgac cttttacgat
      901 gtcaatatca cacgcgcgcc ctttagcgac ctgcgcgtcc gtcaagccct atcgcttgcc
      961 atcgaccgcg agcgtttgag cgcggacacg ctcggcggcg caaccgaagc tgccgcaaag
     1021 tttctacctt cgaccgcagc gaagagcagt gaaaacaatg cggggataga gagcagcgtg
     1081 acattcgatg ccgaacgcgc gcgccgtctg ttcgccgagg ccggtttcgc gggcggcgaa
     1141 aacttcccgc gcctgcgcct gctggtgaat cgcaacaagc agcacagaac ggtggctgcc
     1201 gccgtcgctg cgatgtggcg gaacgttctg ggagtcgaga cggagatcat ggtgcgcgat
     1261 tgggacgagt acgaagcggc tctgcgcgca ggcgattacg acgtagcgcg gcgcagcatg
     1321 gtgatgcaga cagttgacga ggagacaaac atgctcgcca tgttcgcgcc tgacaggctg
     1381 agcgcggcag ctaatgcgga tacgaacggc ggcgcgagca cagaggcgca ggcggctccc
     1441 tcgccgcgcg cggaacctcc cggcgggact gcggcggctg gcggggaaga gtcgcagggc
     1501 ggggcgtcgt tagccgttgc cgcgcaaccg attctgagcg aacagcaggc gctcaaagaa
     1561 ctgcccgcgc tcccgatcta tttcgcctcc tcttacgcgc tcgtcaaacc ttacgtcgcg
     1621 ggcttcgact ccaacctgct cgacgcgcct tcactgcaac acgtacgcat cgagacgaac
     1681 tggcaaccgc cgaaacgcga cacgcccatc cgcatcgtca gcaaccgcta gcgcgtctta
     1741 ctgtaaaagt tttctgttca cgcccttgac ccattgccat agatacactc gatgagccac
     1801 cccgcacccc tcctgtcagt cagaaactta tgcacctatt tcgggagcgc ggacggcgtg
     1861 gtgaaagccg tggacggcgt gagcttcgac ttgaggcgcg gcgagacgtt ggggatcgtc
     1921 ggcgaatcgg gttcgggtaa gtctgtcacc agcctctcgc tgctccggct gatccctgag
     1981 ccgccgggaa aaattctccg tggcgaggtc gtattcgacg ggcaggatat tctctcgctg
     2041 cccttgagag ccgtccggca gattcggggc aagcgcatcg gcatgatctt tcaagacccg
     2101 atggcctcgc tcaatccctt catgcgcgtc tccacgcagt tgatggaact cacccgacta
     2161 cacctgcgcc acacgaagag agaggcgcac gagcatgccg tgcggatgct cgacaccgtg
     2221 ggcatccccg acgcacgcgt tcgggcagag agctacccgc acgagttctc cggcgggatg
     2281 cgtcagcgcg tgatgatcgc gatggcgctc tcttgccagc ccgaactgtt aatcgcggac
     2341 gaaccgacca ccgcgctcga catgacgatt caggcgcaga ttctcgaact cgtcaaaagt
     2401 ttgaaaagta agacgaattt gagcgtcatt ctcatcacgc atgatctggg cgtggtggcg
     2461 ggcatgacgg atcgcgtgat cgtgatgtac gcgggaaaag ttttcgaaga ggctccgacg
     2521 cgcgaactgt ttgcgcgccc ggccaatcct tacacgaaag cgctcctgcg ctccgtgccc
     2581 gacccggaat cggcgcaggg cgcgctctac cagattccgg ggcatccgcc ggacgtttcg
     2641 cgcctgcccg ccggatgccc cttcgccccg cgctgtgaac gcgccgaaga ggtttgccgc
     2701 agagagtttc cgcccttcgt gcaacttacg cgcgaacact cttcactctg tcacttcgcc
     2761 gcagaagtct acgcgcagac gaacgagcgc caagcatgaa cgcacacgac gacacacaga
     2821 cccattttgc tgacgcttcg cacacgctcc ccgcagatgt cgcggcggca ggcggggagc
     2881 cgctcgtcgt ggtgcgcgat ctcaaagtcc atttcgcgca gggcgggggc ggttcgctcc
     2941 gggacaaatt tgcgggcggc actttggcga gacggggcgg tgaccggagc atgagccagg
     3001 gcgagagcca gcaagagggt cggggcgtgt tgaaggctgt ggacggcgtt tcgttcgaga
     3061 tcaagcgcgg agagactctg gggctggtcg gcgaatcggg ctgcggcaag tcaacgctcg
     3121 gtcgcgctct gctgcggctc gcgccggtaa cgagcgggcg actctttttt cgcgggcagg
     3181 aactcacgaa tttgtcgtcg cgcgagttgc gccgttcgcg gcgttacatg cagatgatct
     3241 ttcaagaccc ttacgcctcg ctcaacccgc tgatgaccgt cgggcagatc gtctcggaac
     3301 caatcgagac tttccggctc gcaagcgggc gggagcgcgc cgaacgcgtg cgcgagttgt
     3361 tggagacggt cggcctgagc gcacgccacg ccgggcgtta cccgcgcgaa ttttccggcg
     3421 gtcaacgcca gcgcatcggc atcgcacgcg ccctcgccgc cagcccggag ttcatcgtcg
     3481 cggacgaacc gatttccgct ctcgacgttt ccattcaggc gcagattatg aatttgctgg
     3541 aacgcctgcg gagcgagcgc aagctgacat atcttttcat ctcgcacgac ctgcgcgcca
     3601 tccgccacgt ctccgaccgc atcgccgtca tgtatctcgg caaactggtc gaactcggcg
     3661 cggcggggag cgtctacgac gatccgttga tgccttacac gaaagcgctg atctcggcgg
     3721 tgccgtttgc cgaccccgta cgcgaagccg cgcggcgtcg catcgtgctc gcgggcgacg
     3781 tgccctctcc cctcaacccg ccgcgcggct gccgttttca cacgcgctgc ccctacgcca
     3841 ttcaggcgtg ccgcgaagtc gcgcccgcgc tcgccgaaat caaaccggcg cacttcgccg
     3901 cgtgtatccg catcagcccg gaagaaccgt ccatcgagcg cgtcgcagac gaggccgcgc
     3961 ccggtctgaa aaatcccgct caatttgccg gaacgtgaaa aattgtgtcg attcgtcgtt
     4021 tacacgaaga gtcctgggac acttcctttt gctttgggcg atgatttaac cggccgtggt
     4081 taaatctttc tatggaatat acttgtctct tggcaacggg gcacttatac tgctgaaagg
     4141 ggttgtcccc atcagcctga cccccgtatt gtccggttaa gatgtggcgc acgattgctc
     4201 ctcatcgccg gactgtacac tcaagactcg ccccttcaac cacgcaaggg aacaccccga
     4261 aacgcaaccg gattcagcat ggaaaagtct gccgctacag agcgtgccaa attccggaac
     4321 gaggaactgc caattctcca ggagtgggag cgttggctgg cttcggtcac cgctgaaacc
     4381 gatctcgcga tcctgttggt cgaaggtcat cagccgccgc aactcgccat ctccaacaac
     4441 aactccatct gccacgcttt tcaaagttcg acgacgcacg cgcacctctg cgacccctac
     4501 tgcggcgtcg ctttccagcg cgcgcacgag gcgggagaag cgactcatta tcgctgccac
     4561 gccggactcc actgtttcgc catgcccgtc gggatggacg cgtcgcgccc gctggccgtc
     4621 atcggcgggc gggcttttct ttcgagcgcg gattatcgcg aactcgcaga gcgttttcgc
     4681 cacggcgacc tgcgtgaact gctctccacc gaactcttcc gcaacgttat cttcgcctcg
     4741 cgccatcatc tcgatgaact ggccgcccaa ctggccgaag cctccgtgac ggcagacgca
     4801 cgccgcgcaa ccgggagcag cccgaccaat aaaggcgcgc acacggaaac gccgcgcgag
     4861 caggcagccg tggaagcgaa gggaggggaa ctatcgcgcc cgcgcggcgt caatttcccg
     4921 cccggcctga agttgctcga cgcttgccag atcgcgctcg aaaagttgcg cgtgagattc
     4981 aagttgaagt cggtcgtcct gatgctgcgc acgggcgacg atttcacgcc cgcgtgcggg
     5041 atgggcaagt tcaagaacag atcgccgcaa ctatccgcga agggcaagag cgacgagcac
     5101 ctgaaactgg tgggcggcac ggatgcgagc ggcgtggcgg cggagttgtt cgcactgagc
     5161 gacgatgaga cggaggtttt cccgctcgtc gtcggcgacg agttgaaggg cgcgctcctt
     5221 gtcgccgacg gcaaattgcc ggaagagaaa cggcgtggct tgagcgagtt ctgccttgag
     5281 gtcgccatgc cgctcgaagt cttgcggctg cgcgaggagt tggaacgcag cctgcgcgcc
     5341 agctatcacc tgcaagcctt cacgcagcag atcaacgaag tcgagcctgc cgaagcctac
     5401 gccgccatcc tgcgccattc gaccgaactg ttgcgctccg aacgcggctc gctcctcttg
     5461 tttgacgaag cggcgaatga actggaagtg aaagcggcgg tcggacccag agccgacgtg
     5521 acgcaggacg agcgcgtgcg cgtcaacgag agcgtctccg gctcggtgct gctcgacgga
     5581 cgcccgcgca tcgtgcgcga tctgcgtacg acggaactgc atcgcgctcc cgccgagaga
     5641 aagtataaga gcgattcttt catcagctac ccgatcatca cgggcgggcg caaagtcggc
     5701 gtcatcaaca tgacggacaa agcaggcggc gagccttaca acgatttcga cctgcacctg
     5761 ctcgaaacga tggtgccgca gatggcgctc gcgctcgacc gcgccggttg gcatcagaaa
     5821 gcgacgcagt ttcaactgct ctccatcacc gacccgctga ccggcctgct caaccggcgt
     5881 tatctcgaag agcgtctggg cgaggagttc gagcgttcca agcgtcatcg tttcccgatg
     5941 agctttctga tggtggatat agataatttc aaggagtaca acgaccggaa cgggcatcag
     6001 gcgggcgacc tcgccttaga gatcacggcg caatgtttga agtccgcgct gcgttcggcg
     6061 gacgtggcct cgcgctacgg cggcgaagag ttcagcatcc tcctgcccca gacgaatatg
     6121 gcggaagccc tcgtgatcgc cgaacgtatt cgcagccgcg tggagaaatc gtcctacccg
     6181 cacggcgaca gccagccgca cggcgcggtt acggtcagca tcggcgtctc ggccttcggt
     6241 cccaaactcg aaacccctgc cagcatcatc ggcgcagccg accacgcgct ttacgtcgct
     6301 aagagccggg gcaaaaactg cgtcgtctct tacgtccttc cccccaacac atcccccgcc
     6361 cgcgatgtca actgaaaacc tcttcaaggc cgccccggcc aaacgcatac tgggtcgcgt
     6421 cgccccggaa aactttatcg ggcgaagcga gcacttgcgc gaaatcagcg cgctcgccac
     6481 gcagttgtcc gagcgtcgcg gactgctcat ccacgccgcg cccgccgccg gagcgtcgga
     6541 acttctccgg caggcttacg ataaactttt ccagcaacgc ggcggcgcgt ctccgatcta
     6601 tttcgccttc acgcggcagg agcagagcat tgaggctgcc gcgcgccgct tcctccacac
     6661 gtttctcacg cagctcgtcg cgcaccgtcg ccacgactcc tccttcgtca atgcgccgcc
     6721 cgccctgcgc gatctagtag acctcgcaga ccccgcggat tacgaatgga tcgagcgtct
     6781 catcgtgact tatgaacgtg cgcgcgaagg cacggacgaa ctcgcgctcg tgcgcctctg
     6841 tttcagcgcg cccgtgctcg cggcggcgcg cggcgcacgc acggtcatcc tgatagacga
     6901 gatacagaac atcgaaaagt tgagaggcga agtcgaactc ggcaacgaga tcgcgcaagt
     6961 ctcgatgcac ggcgacgtgc cattcgtgtt gtgcggactc cggcgcgggc tgctcgacga
     7021 attgagcggc ggcaacgccc cgcaccgttt cgacggcttc ggcaccatcc atctggaagg
     7081 cttgaaggaa gcggacgcgc gcacgctcgt cgagcgtctg gcctacacgg aacgcgtcgc
     7141 gctcaacgac gagacacgcg atctcatcgt ccaactcttc aacggcagcc ctttttacat
     7201 cacgtcgttt atgtacgcgg cgcaccgcga aggagtctcg ctcaccagtt ttcttgagtg
     7261 tcagaaaatt tacgtggacg aagttttggg cgggcgcatc aatcgccgtt tcaatgccat
     7321 cct
//
LOCUS       DASWNS010000438         7221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_68187, whole genome shotgun sequence.
ACCESSION   DASWNS010000438 DASWNS010000000
VERSION     DASWNS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68187"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VGB76_15095"
     CDS             <1..864
                     /locus_tag="VGB76_15095"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0100275.1"
                     /translation="PGIGMRINGQQCWIRMPVVGQFQPSEFVKIPTVLMLAYYFGRQR
                     QGALGWFQIAVGVGILALPVVLILLEPDAGQVITYVPLLAVVLFLSSVRMWLVLAALA
                     LSVVLIPAAYLVGVKTGKIKNYQQQRIQAILDPEKADRRGYGYQTYQSILSVGKGGVA
                     GTGGGSTEYSQSRLKFLPEPHSDFIFAVTAERTGFVGSILLLVAYAVLLSRLLMGARR
                     APDRAGMLVIMAIVGGLAFQIFINIGMALGILPVIGVPLPMMSAGLSSVLATFIAIGF
                     AVSVQLRRFVN"
     gene            complement(974..1591)
                     /locus_tag="VGB76_15100"
     CDS             complement(974..1591)
                     /locus_tag="VGB76_15100"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY0100276.1"
                     /translation="MKRIFGLLVFVAVFSALAGVVQADPLVFTATLSGANEVPQTSSP
                     GTGFAVVTVDGNIMTVTANFSGLVTTTANGSPSGTTAAHIHCCAPATGTAGVATPVPT
                     FPGFPSGVTSGTYTNSFDLTLASSYNPAFIAARGGTVEAARLAFLNGLQNGQTYFNIH
                     SNAFPGGEIRGQLQPVPEPTTLVLLGTGLAGAVLRSRRKRKGVAA"
     gene            complement(1785..2585)
                     /locus_tag="VGB76_15105"
     CDS             complement(1785..2585)
                     /locus_tag="VGB76_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100277.1"
                     /translation="MKPSYGRLAAPLLVAALALISPASLNAPASAQPAAPAFAQQCPT
                     VTVSCPDTARENLEDALTFTANVSGGDPNVTPTFNWTVSAGSISSGQGTSSIKVDTNG
                     IGGQTVTATVDVGGFARECRTSNSCTSSIEKKSTPAVKFGEYVTRDLSANKAQLDKFV
                     LALQQDPTAQGYLIAYGGRTSRPEDAQKAADNATDYTINTRKMDGARTLSGVGGYREQ
                     PTIELWIAPPGATPPLATPTIRPEDVKPAPANPEKKPATTNTPKGKKS"
     gene            3574..6792
                     /locus_tag="VGB76_15110"
     CDS             3574..6792
                     /locus_tag="VGB76_15110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY0100278.1"
                     /translation="MSKELIVSVNGREKKIAIIENGKVTEFYIERGEDGQGIVGNIYK
                     GRVMRVLPGMQSAFVDIGLERDAFLYVSDFFDEEEEFERIVVNKTKTEGADATTAARA
                     AAEKIERSRLERERQMETAQERAQPILTDGEEEEEDDEATELEEPRAAMQTESATPPL
                     SASSVNADEEDEGATETRGGRGRRDRKRRGGGGREDAQREVGAAASAAGGGVSGSNIA
                     PPNTSFTEDVQARLAAREEIDTPFVAADNSFERIIDDEAAATNGEMFKDARLQERLTD
                     QIHAIEFDMETTRDAEVGSLLSSENISAGSSAFQRIADDSDDDISAPRSVEAVQPAVA
                     ATVSAFIEETDDSALTGVQAQSFERVSDDDKPAEGVPTEAGTEEATTSARSKASKSRR
                     AASKGKKAASDETADETAAATEETGAKKGGRSRASKEAASKKSATKGKSTAATKGGST
                     RRGSKAKAGSEAGSEAAADATTEGNAPDDAAAATQTSPDAEAGVKSRRSRDEFARRGG
                     RRRNRRPGGGAPGENGGEEDRSNGEAEEIIAPVELEAEAVEEVQEARPEPSRAETRPE
                     PRPESRPESRPDPRGRQQARGGRNERGDRGDRGGRSDARSPRPERGGGDGGGAERGER
                     TDAPRAGGEASGGRGDGGGRPDGGGRSGGGGGGRDRRERHTPLITDLLREGQEILVQI
                     AKEPIAAKGARITSHIALPGRFLVYMPTVEHVGVSRKIESDSERQRLRKLIKDIRETE
                     DVPSGGFIVRTAGIGISEQDLRDDARYLVRTWLDIRRTAEKQKAPTLTHKDLDLVQRI
                     LRDQLSDDFAAIRVDSEEEYQNIVEFINRIQPRLVKRVKLYTREEPILEAFGVQAEID
                     KAIKPRVWLKSGGYLVINQTEALVAIDVNTGKFVGRGGTRLEDTITRTNMEAVEEIAR
                     QIRLRDLGGIIVLDLIDMEDRRNRNRVMGALQDALRDDKSPTKVLSFNDFGLVIMTRK
                     RVKQSLERTLCSPCPYCQGAGLVKSAQTVCYEILDEARRLSRGMNGDGIKQTTLRINP
                     EVSRALRSTERDVLNEIEDYLGAVDITSDERVHQEQFDFAFI"
     gene            6875..>7221
                     /locus_tag="VGB76_15115"
     CDS             6875..>7221
                     /locus_tag="VGB76_15115"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0100279.1"
                     /translation="MSVKQKFWQGLFLAGGAGVAALAAVNASIARGVATETEDDALGG
                     EAREFAFEHGRVFYRVNGISEGREPLLFLHDIGAGASSFIWRKNFDALAADFQTYAPD
                     LPGFGFSDKPADAP"
BASE COUNT         1403 a         2231 c         2349 g         1238 t
ORIGIN      
        1 cccggcatcg gcatgcgcat caacgggcag caatgctgga tcaggatgcc ggtcgtaggg
       61 cagtttcagc cctccgagtt cgtcaagatt cccaccgtgc tgatgctcgc ttactacttc
      121 ggcagacagc ggcagggggc actaggctgg tttcagatcg ccgtcggggt cggcatcctt
      181 gcgctccccg tagtgttgat cctgcttgag ccggacgcgg gacaggtcat cacttacgtg
      241 ccattgctcg ccgtggtgct ctttctgtcg tcggtgcgca tgtggctggt gctcgccgcg
      301 ctcgcactga gcgtggtgct cattccggcg gcctacctcg tcggcgtgaa gaccggcaag
      361 atcaagaact accagcagca gcgcatccag gccatcctcg atcccgaaaa agccgaccgc
      421 aggggctacg gctaccagac ttatcaatca attctctctg tcggcaaagg cggcgtggcg
      481 ggcacgggcg gcggctccac cgaatactcg cagagccgct tgaagttcct ccccgaaccg
      541 cacagcgatt tcatcttcgc cgtgacggcg gagcgcacgg gcttcgtcgg ctcgatcctc
      601 ttgctcgtcg cctacgcggt gctgctctcg cgtctgttga tgggcgcgcg ccgtgcgccc
      661 gaccgcgcgg gaatgctcgt catcatggcg attgtcggcg gcctcgcctt tcaaatcttc
      721 atcaacatcg gcatggcgct cgggatactg cccgtcatcg gcgtcccgct gccgatgatg
      781 agcgcgggac tctcctccgt cctcgccacc ttcatcgcca tcggcttcgc cgtcagcgtc
      841 caactgcgcc gcttcgtcaa ctgacaggtt agacaaaacg atagcggagt gcagcggcac
      901 agagagttct ctgccgttgc actccgctat caagctacag gatgaaaatg tgcgcgacgc
      961 ctgaaagcgc aagctacgcg gcgacgccct tgcgcttccg gcgactgcgc aagaccgcgc
     1021 ccgccagccc cgtgccgagc aggacgagcg tggttggttc ggggacgggt tgaagttgcc
     1081 cgcgaatctc gccgccgggg aaggcgttgc tgtgaatgtt gaagtaggtc tgcccgttct
     1141 gtaagccgtt gaggaaggcg aggcgcgccg cttctaccgt cccgccgcgc gcggcgatga
     1201 aggcggggtt gtaactggaa gcgagcgtca ggtcgaaact gtttgtgtag gtgccggagg
     1261 tgacgccgga gggaaagccc ggaaacgtcg gaaccggcgt ggcgaccccg gctgtgcccg
     1321 tcgccggagc gcaacagtga atgtgcgcgg cggtcgtgcc gcttggcgag ccgttggccg
     1381 tggtggtgac cagcccgctg aagttggcgg tgacggtcat gatgttgccg tccactgtga
     1441 cgacggcgaa gcccgtgccg ggagaactcg tctgtggcac ttcgttcgcg ccggagagcg
     1501 tcgcggtgaa gaccagaggg tctgcctgca ccacgcccgc caacgccgag aagaccgcca
     1561 caaagacaag caggccgaaa atccttttca tcgcatacct ctttctctgt ttaactttta
     1621 gccgcacgat taggccgcaa aaactactct ctttccgttt tcgagtcaac attttttcgc
     1681 aaggcggcga ccggctttca acactccttt aataaattta aggggcagcg tgcagatctg
     1741 cacgctgccc cttggatgga gttcgtcttg aagcgttcgc ggctttacga ttttttaccc
     1801 ttcggggtgt tcgtcgtcgc aggcttcttc tcggggttcg cgggggctgg ctttacgtcc
     1861 tccgggcgaa ttgtgggcgt cgccagcggg ggcgtcgcgc cggggggcgc gatccagagt
     1921 tcgattgtcg gctgctcgcg gtagccgccg acgccggaca gcgtgcgtgc gccgtccatc
     1981 ttccgcgtgt tgatcgtgta gtcggtcgcg ttgtcggcgg ccttctgcgc gtcctctggt
     2041 cggctcgtgc gtccgccgta ggcgatgaga tagccctgcg cggtagggtc ttgctggagc
     2101 gcgaggacga acttgtcgag ctgcgccttg ttcgcgctca ggtcgcgcgt gacgtactcg
     2161 ccgaacttga ccgccggggt ggacttcttc tcaatcgagc tggtacacga gttggaagtg
     2221 cggcattcgc gcgcgaagcc gccgacatct acggtcgccg tcaccgtctg cccgccgatc
     2281 ccgttcgtgt ccaccttgat cgaagaagtt ccctgccccg aactgatgga gcctgcggag
     2341 acagtccagt tgaaggtcgg cgtcacgttc gggtcgccgc cgctcacgtt ggccgtgaag
     2401 gtcaaagcgt cttcaaggtt ctctctggcc gtgtccgggc aagagacagt gacggtgggg
     2461 cactgttggg cgaaagccgg ggcggcgggc tgcgccgaag cgggcgcgtt taggctggcg
     2521 ggggagatca acgccagcgc agccaccaac aagggcgcgg ccagacgacc gtaacttggt
     2581 ttcattctaa tgttctcctg aaagttgaaa tttatgagaa cggttgatcg cgtgcgaaag
     2641 aagcggaaga acttaagacg gcgcgaaact tacgtgcccg ccgggctgac ttcgcaagac
     2701 attatcttcg aaatctcgca gaggtcaacg acgggctgcg tgcctgagga actgtgcgtg
     2761 cgtgccgcct gcgtcgtctc cgccgcctca gcgctatctt tactcgcgtt tcacgaggct
     2821 ctcggttata cttcacttgc ctcgtcctac cggaatttgt tggcgcggtg tagaaggacg
     2881 cgcttccagt cgcgagaaga ttcaatcttg cactccgcac agacgagaca catttttttt
     2941 gcagcgtcgc gggcaatggt tccgcgattg ttgatgagat atttgaagcc gttgatgggt
     3001 cgtcgggcgt gcgcgttcct gcgttgcgcg acgttaagac catctgctgt tgctggcaag
     3061 agtcgagaaa cgacaggccg gggagcatgc gtgtctgcgt aagcgagttt gacgctcgcg
     3121 cgccaccgcc gccattgctc acgcgccgag acgcatcttc ggacgctcgc acaacgcgca
     3181 ctagaggacg gctcgacgga gtttcaggtg aacagtccaa atcgcttttc cgctccagcg
     3241 gaagcggcgc ctccaagtgc cgggcacaca cagcccgagc cgcgtgaagc gcgcttcccg
     3301 ttccataccg cagaatggtt gtttgtgttg cgcacgcgtc ccgccgtcgg ccttccacgc
     3361 ccgaaattca tctcatctct catctgcgca cgccgacccg tcggcgtcgc ttccgacttg
     3421 cgaggtactt aaacgcctgc gctccacacc gaacggaatg agagcgcacc cttattgcgg
     3481 cgacaacttg cccgctccat ttccatatca agaccgccgc cagccgcatg aaacgtgcgc
     3541 cgccggttcg cgtcaggagt caaaaatttt ctcatgtcga aagaattaat cgtcagcgtc
     3601 aacgggcgcg aaaagaagat cgccattatt gagaatggca aagtgacgga gttttatatc
     3661 gaacgcggcg aggacggaca gggcatcgtc ggcaacatct acaaggggcg cgtgatgcgc
     3721 gtgttgcccg gcatgcagtc ggcctttgtg gacatcggcc ttgagcgcga cgccttcctc
     3781 tacgtctcgg acttcttcga cgaggaagaa gagtttgagc gcatcgtcgt caataagacg
     3841 aagacggagg gcgcggacgc tacgaccgcc gcacgcgccg ccgcagagaa gatcgaacgc
     3901 tcgcgcctcg aacgcgaacg ccagatggag acggcgcagg aacgcgcgca accgattctc
     3961 accgatggcg aagaggaaga agaggacgac gaggcgaccg aactcgaaga gccgcgcgcg
     4021 gccatgcaga ccgagagcgc gacgccgccg ctgtcggctt cgtcagtcaa cgccgacgaa
     4081 gaggatgaag gcgcgacgga gacacgcgga gggcgcggca ggcgcgaccg caaacgtcgc
     4141 ggcggcggag ggcgcgaaga tgcgcagcgc gaagtcgggg cagcggcgtc ggcagccgga
     4201 ggcggcgtca gcggcagcaa catcgcgccg ccgaacacga gtttcacaga ggacgtgcag
     4261 gcgcgtctcg ccgcacgcga agagatcgac acgcctttcg tcgcagcgga caactccttc
     4321 gaacgcatca tcgacgacga ggcggcagca accaacggcg agatgttcaa agacgctcgc
     4381 ttgcaggaac gcctcacaga ccagattcac gccatcgagt tcgacatgga gacgacgcgc
     4441 gacgccgaag tcggctcgct tctctcctcc gaaaacatct ccgcaggcag cagtgccttc
     4501 caacgcatcg ccgacgattc cgacgacgat ataagtgcgc cgcgctcggt cgaagccgtg
     4561 caaccggcgg tggccgcaac cgtctccgcg ttcatagagg agacggacga ttccgctctg
     4621 accggcgtgc aggcgcaatc gttcgagcgc gtgagcgacg acgacaagcc cgcagagggc
     4681 gtgcctacag aagcgggcac agaagaagcg acgacttccg cgcgcagcaa ggcgagcaaa
     4741 tcccgacgcg cggcgagcaa gggcaagaag gccgcgagcg atgagacggc ggacgagacg
     4801 gcggcagcga ccgaagagac gggcgcgaag aagggtggcc gttcgcgtgc gtcgaaagag
     4861 gcggcgagca agaagtctgc gaccaagggc aagtcaacgg cggcgacgaa gggcggctcg
     4921 acgcgtcgcg gcagcaaggc caaagcgggc agcgaggcgg gcagcgaggc ggcggctgat
     4981 gcgaccacgg aaggcaatgc tcctgacgat gcggcagccg cgactcaaac gtcgcccgac
     5041 gcggaagcgg gcgtgaagtc cagacgctcg cgcgacgaat tcgcacgacg cggcgggcgg
     5101 cggcgcaatc gtcgccccgg cggcggtgcg cccggagaga acggcggcga ggaggatcgc
     5161 tccaacggcg aagccgaaga gatcatcgcg ccagtcgagt tggaagcgga ggcggttgaa
     5221 gaagttcagg aagcgcgccc cgaaccgtca cgcgcggaga cgcgtcccga accacgcccc
     5281 gaatcgcgtc ccgaatcgcg tcctgatccc agaggacggc agcaggcgcg cggcgggcgc
     5341 aacgagcgtg gagatcgcgg agatcgcgga ggacgcagcg acgcgcgcag cccacgcccc
     5401 gaacgcggag gcggcgacgg cggcggagcg gaacgcggcg agcgcacgga cgccccacgc
     5461 gcaggcggcg aggcttcggg cggtcgcggc gacggcggag gtcgccctga cggcggaggg
     5521 cgtagcggcg gaggcggcgg aggccgcgac cggcgcgagc gacatacgcc gctcatcaca
     5581 gacctcctgc gcgaaggtca ggagatactc gtccagatcg cgaaagagcc gattgcggcg
     5641 aagggcgcgc gtatcacgtc gcacatcgct ctgcccggtc gttttctcgt ctacatgccg
     5701 acggtggagc atgtcggcgt ctcgcgcaag atcgagtcgg acagcgagcg acaacgcctg
     5761 cgcaagttga tcaaggacat ccgcgagacg gaagacgtgc cttcgggcgg cttcatcgtc
     5821 cgcaccgcag gcatcggcat cagcgagcaa gacctcagag acgatgcgcg ctatctcgtc
     5881 cgcacgtggc tcgacatacg ccgcaccgcc gagaagcaga aagcgcccac gctcacgcac
     5941 aaagacctcg acctcgtcca acgcatcctg cgcgatcaac tctcggacga cttcgcggcc
     6001 atccgcgtgg attccgaaga ggaatatcaa aacatcgtcg agttcatcaa ccggattcag
     6061 ccgcgactcg tcaagcgcgt caaactctac acgcgcgagg aaccgatcct cgaagccttc
     6121 ggcgtgcagg ccgagatcga taaagcgatc aagccgcgcg tgtggttgaa gtcgggcggt
     6181 tatctcgtca tcaatcagac ggaagcattg gttgcgattg acgtgaacac gggcaagttc
     6241 gtcgggcgcg gcggcacgcg gctcgaagac acgatcacgc gcacgaacat ggaagcggtc
     6301 gaagagatcg cgcgccagat acgcctgcgc gacctcggcg gcatcatcgt gctcgacctc
     6361 atcgacatgg aagaccgccg caaccgcaac cgcgtgatgg gcgcgctgca agacgccctg
     6421 cgcgatgaca agtcgccgac gaaggtgctc tcgttcaacg acttcggcct cgtcatcatg
     6481 acccgcaagc gcgtcaagca gtcgctcgaa cgcacgctct gctcgccgtg cccgtattgt
     6541 cagggcgcgg ggctggtcaa gtctgcgcag accgtctgct acgaaatcct cgacgaagcg
     6601 cgccgcctct cgcgcgggat gaacggcgac ggcatcaaac agacgacgct gcgcatcaac
     6661 cccgaagtct cgcgcgccct gcgctcgacc gaacgcgacg tgctcaacga gatcgaagac
     6721 tatctcggcg cggtggacat cacgagcgac gagcgcgtcc atcaggagca gttcgacttc
     6781 gcgtttattt aaatgcagaa gtcagaggtt agaggttaga agagatcagt tattgcgttt
     6841 actgatctct tctaacctct gactacctgt ttttatgagt gtgaagcaaa aattctggca
     6901 gggacttttt ctcgcgggcg gcgcgggcgt ggccgcgctg gcggcggtca atgcctcaat
     6961 cgcgcgcggc gtcgcgaccg aaacggaaga cgacgcactc ggcggcgagg cgcgcgagtt
     7021 cgccttcgag cacgggcgcg tcttttatcg cgtgaacggg attagcgaag ggcgtgagcc
     7081 gctcctcttt ttgcacgaca tcggcgcggg cgcatcgtcc ttcatctggc gcaagaactt
     7141 cgacgcgctc gccgcagact ttcaaactta cgcgcccgac ctccccggct tcggcttctc
     7201 cgacaagccc gccgacgcgc c
//
LOCUS       DASWNS010000439         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_326761, whole genome shotgun sequence.
ACCESSION   DASWNS010000439 DASWNS010000000
VERSION     DASWNS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326761"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VGB76_15120"
     CDS             <1..699
                     /locus_tag="VGB76_15120"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0100280.1"
                     /translation="DKAEAAGLPEIDIGVKRDVDVPLSECGEEQSEALGHWFKKMSPE
                     ERPTVVLTSPYIRARQTSEHVLEAAGISKDEITFIADERLREKEFGILDRLTKFGIEA
                     KYPDQLAFRSAIGKFYHRPPGGESWCDVILRLRNVIDTLTREYRRERVLIVAHSVVVL
                     CFRYLLERMTEEQILSIDRATDVANCSVTSYVYDAGIGKNGKLILQQYNFVAPLEREG
                     APVTTDKDVPVAPK"
     gene            728..1675
                     /locus_tag="VGB76_15125"
     CDS             728..1675
                     /locus_tag="VGB76_15125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY0100281.1"
                     /translation="MTEKSVSGSRRAGETRVRPQVITPKLLGAWPVPQPEEDDDKEAR
                     GRVLVIGGSTEIAGAVVLAATAALRAGAGKMQIATCRSVAAHVSMAVPEARVFALPET
                     RAGGIAAGGFKQIEEQLAGAQSILVGPGMIDPAATARLLKNILPLIERAALVLDAGAL
                     SFLAEQPQSLARLHGRVVLTPHAGEMASLLGVEKEAVEADSLETVRHAVTKLGAVVAL
                     KGRETLIAAPASRNVYCNRAGNVGLATSGSGDTLSGVIAGLAARGSTPFQAAVWGVYL
                     HARAGDELARRMGSLGFLARELLAEIPPLMAKLSPARKR"
     gene            complement(1770..2945)
                     /locus_tag="VGB76_15130"
     CDS             complement(1770..2945)
                     /locus_tag="VGB76_15130"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0100282.1"
                     /translation="MLRHMLLTTALLCSGLAAFFTVWIVVPAPSYSLFQVAVGASEWS
                     LWFGALGLAGACLGLFALAAGSRRTGWAALSLGAAAVALAMIPTLSARRVARENNIEL
                     SLRQYLFGRPEAPPASSERTSETFASVEGQTLRLDVYLPMPKAARKRAAIVVVHGGSW
                     NAGERGDYPQWNEWLAGQGYVVFDIDYRLAPQPNWRTATGDVKCAILWVRANASRYGV
                     DEARIALLGRSAGGHLALLAAYSTGEASLAPSCAVSASGSPVRAVVSFYAPTNLEWGF
                     AHPANERVLDGRGRIRDFVGGTPESAAEAFKLASATSHVRAQTPPTLLFHGGRDQLVR
                     DENMALLAARLAQENIPHRAVLIPYAQHGFDYNFNGWGAQIARQLMSDFLRAHLGEN"
BASE COUNT          593 a          982 c          971 g          508 t
ORIGIN      
        1 gacaaagcgg aggcggcggg tctgcccgaa attgatattg gcgtcaagcg cgacgttgac
       61 gtgcctctgt ccgagtgcgg cgaggaacag tcggaggctc tcggccactg gttcaaaaag
      121 atgtcgcccg aagagaggcc gacggtggtg ctcacctcgc cctacatccg cgcccgccaa
      181 acctcggaac acgtcctgga agccgcggga atctcgaagg acgagatcac cttcatcgcc
      241 gacgagcgtt tgcgcgaaaa agagtttggc atcctcgacc gcctgacgaa gttcggcatc
      301 gaggcgaagt atcctgacca gttggctttt cgcagcgcga ttggaaagtt ttatcaccgc
      361 ccgcccggcg gcgaaagctg gtgcgacgtg atcctccgcc tgcgcaacgt gatcgacacg
      421 ctgacgcgcg agtacaggcg cgagcgcgtg ctcatcgtcg cgcattcggt cgtcgtgctc
      481 tgctttcgct acctgctcga acggatgacc gaagagcaaa tcctgtccat agaccgcgcc
      541 actgatgtcg ccaactgctc cgtcacgtct tacgtctacg atgccgggat agggaagaac
      601 ggcaagctca tcctccaaca atataatttc gtcgcgccgc tcgaacgcga aggcgcgccc
      661 gtcaccactg acaaggacgt tccggtcgcc ccgaaataga ctcgccgccg acgtgaaaag
      721 agagacgatg acagaaaaaa gcgtttcggg ttcgagacgc gccggagaga cgcgcgtgcg
      781 gcctcaggtc atcacgccga aactgctcgg cgcgtggcct gtgccgcagc ccgaagagga
      841 cgacgataaa gaggcgcgcg gtcgcgtgct cgtcatcggc ggttcgacgg agattgcggg
      901 cgcggtcgtg ctggccgcga cggcggcttt gcgtgcgggc gcgggcaaga tgcagatcgc
      961 cacctgtcgc agcgtcgcag cgcacgtctc gatggccgtg cccgaagcgc gcgtgtttgc
     1021 tttaccggag acgcgcgcgg gcgggatcgc ggcgggcggc ttcaagcaga tcgaagaaca
     1081 gttggcgggc gcacagtcaa ttctggtcgg acccggcatg atcgatcctg cggccaccgc
     1141 gcgcttgttg aaaaatatcc tgcctctcat cgaacgcgcc gcgctcgtgc tcgacgcggg
     1201 cgcgctctcc tttctcgcag agcagccgca gagccttgcg cgcctgcacg gcagagtggt
     1261 gctgacgccg cacgcgggag agatggcgag cctgttggga gttgagaagg aggccgtcga
     1321 agccgattct ttggagacgg tgcgccatgc cgtgacgaaa ctcggcgcgg tggtcgcttt
     1381 gaaagggcgg gagactttga tcgccgcgcc cgcgagccgt aacgtctatt gcaaccgcgc
     1441 ggggaacgtg gggctggcga catcgggttc gggcgacacg ctctcgggcg tgatcgcggg
     1501 gctcgcggcg cgcggcagca cgccttttca agccgccgtg tggggcgtct acctgcatgc
     1561 gcgtgcgggc gacgaactcg cgcggcgcat gggttcgctc ggtttcctcg cgcgcgaact
     1621 gctcgcggaa atcccgcccc tcatggcgaa actttctccg gcgcgcaaga gatgaacgac
     1681 gccggatgat gaacgaggcc agccgccctc aaccgaactc aagcaaactc aaccacactc
     1741 aagcgattgt ttgaacgaac tggtaagtct taattctcgc ccagatgagc gcgcaggaaa
     1801 tccgacatca actgccgggc aatctgcgct ccccagccgt tgaagttgta gtcgaaaccg
     1861 tgttgcgcgt aagggatgag aacggcgcga tgcggaatgt tctcttgcgc caggcgcgct
     1921 gcgagcaagg ccatgttttc gtcgcggacg agttgatcgc gcccgccatg aaagagcagg
     1981 gtcggcggcg tctgcgctct aacgtgagag gtcgcagacg cgagtttgaa cgcttccgcc
     2041 gccgactcgg gagtgccgcc gacgaaatct cgaatcctgc ctctcccgtc gaggacacgt
     2101 tcgttagccg gatgcgcgaa gccccattcc aagtttgtcg gcgcgtagaa ggagacgacc
     2161 gcgcgcacgg gcgagccgga cgcagaaacc gcgcaactcg gcgcgagcga tgcctcgccc
     2221 gtcgagtaag cggccagcaa ggcgagatgc ccgcccgccg aacgaccgag cagggcgatg
     2281 cgcgcctcgt caaccccgta tctcgacgcg ttcgctctga cccacaagat cgcgcacttc
     2341 acgtcgcccg tcgccgtccg ccagttgggt tgcggcgcga gtcggtagtc aatatcgaag
     2401 accacataac cctgcccggc cagccactcg ttccattgcg ggtaatcgcc gcgctcgccc
     2461 gcgttccagg agccgccgtg aacgacgacg atggccgcac gcttcctcgc cgctttgggc
     2521 attggcaaat aaacgtcgag cctgagcgtc tgcccttcga cgctggcaaa agtttcgctc
     2581 gtccgttcgc tgctcgcggg cggcgcttcc ggtctgccga aaagatattg acggagggat
     2641 aattcgatat tgttctcgcg cgcaacgcgc cgtgcgctaa gcgttggaat catcgccagc
     2701 gcgaccgccg ccgctcccag cgacaacgcc gcccatcccg tccggcgact ccccgccgcg
     2761 agcgcaaaca aacccaggca cgcgcccgcc aaccccagcg cgccaaacca caagctccac
     2821 tcgctcgcgc ccacagccac ctgaaagaga ctgtaagacg gcgcgggcac gacgatccaa
     2881 acggtgaaga acgcagccag accgctacac agaagagccg tcgtgaggag catgtgccgg
     2941 agcatatcgt gcgaacggag acaggctctc gctagtccgc cgtcatcgtc tccggctcgc
     3001 gcgcggtgag ttcgcgccac gtaagcgggc ggctagcatc aatcaattct tgct
//
LOCUS       DASWNS010000440         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_199535, whole genome shotgun sequence.
ACCESSION   DASWNS010000440 DASWNS010000000
VERSION     DASWNS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199535"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1721)
                     /locus_tag="VGB76_15135"
     CDS             complement(3..1721)
                     /locus_tag="VGB76_15135"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HEY0100283.1"
                     /translation="MFRRSLTLLSVCTLALLFSAAPFAVRAQAAQGGDVIIVLPFENT
                     TSQREYHWIGTSFADSLTDLLQMYGLRTISTDERELIYQRLRLPLTATPSRATAINVA
                     MKAKATLAVIGTYDVVTPADGKTPTEVRVSARVVRVNEGQIWSPYIFGDALVNLQKMQ
                     GILAYQILHQRDSTLAYSRNEIVERATKIPPRAFESYVKGVMTEDREKRSAYLQNALK
                     EYAKTNAGASYPQAAFELGTLYFKQEDWKRAAENFTRLQKGDPHFAEASFYAALSYWR
                     MNDLKTALGALLPVASDMPLTGIYNNAGALSIQAALAEKSTEERERLLKQAQTFLERA
                     KESDPEDASVLYNYAYTLFLMNRHADAAEQLRSVIMLNPRDGEARFLYAKSLEKSGAT
                     AEMATAADNEARRYLPTYAKLQTAWQKSATITDVPVRLNQQFDIAEAVKAQAPLPVVD
                     TASVTTQDLLVKARADYAAGRDDDVLSELNRVLMIEPMNAEAHLLIGRIYQRRGDLVR
                     AISSLKTAIFWEAKLIDAHILLGRIFLERNDLAQAKTHARTAIQLDPNNQEAIALYRQ
                     LETGAR"
     gene            complement(1988..3382)
                     /locus_tag="VGB76_15140"
     CDS             complement(1988..3382)
                     /locus_tag="VGB76_15140"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEY0100284.1"
                     /translation="MKLRGYFRCALATTLNIILYALTFETFLWLEGRVRRGFFRNWAR
                     NFRYRPGRFRRPTSEQEIIDIVKNESGIRFFGAGHSFNDGVVADETLVSLDNYSGMVW
                     KDANLKRMCVKGGTRMRDVIKLLLDEGWAFKALPSHDAQSIAGILSTDVHGTGKDWGF
                     VSESVVSLRIIDGAGNVQVCHPADDLFRAAIGGVGAVGIISEVVVQAVERFNVEQNFR
                     MSTRTLVFDPVNFQNLLQENEHFSLYLFPFTDKCQINTWNRTNKPKSPLGTLRELISI
                     SADALLSAWFGNFMAYTGLLPRWSNLSYAFKRGTDLVLESSEAYTRTIYHLHQELEFT
                     VPFEDTLAMCNHFIQLYEQTYTSNTPYALFEIRFTPKDHTWSLIGAGQQGPRTWIDLV
                     INDSYGFENFYGAAEASAKSLGARPHLGKFCTLFDKNDLMNLYQNNFVRFQQLRQQQD
                     PQGKFANVFTRRIF"
BASE COUNT          742 a         1028 c         1227 g          805 t
ORIGIN      
        1 ccttatctcg cgcccgtctc caactggcga tagagcgcga tggcttcctg attgttgggg
       61 tcgagttgga tggcggtgcg cgcgtgggtc ttggcctgcg cgagatcgtt gcgttcgagg
      121 aagatgcgcc ccagaaggat gtgcgcgtcg atcaacttgg cttcccagaa gatcgcggtc
      181 ttcagggaac tgatggcgcg gacgaggtcg ccgcgacgct ggtagatgcg cccgatgagc
      241 aggtgcgctt cggcgttcat cggctcgatc atgagaacgc ggttgagttc cgagagcacg
      301 tcgtcgtctc gacccgccgc gtagtcggcg cgcgccttga ccagcaagtc ctgcgtggtg
      361 acgctcgccg tgtccacgac gggcagcggc gcttgcgcct tgacggcttc ggcgatgtcg
      421 aattgttgat tcagacgcac gggcacgtcc gtaatcgtcg cagacttctg ccaggcggtt
      481 tgcagtttcg cgtaggtcgg cagataacgg cgcgcttcgt tatcggcggc ggtcgccatc
      541 tcggcggtcg cgcccgattt ctccagagac ttcgcataga ggaaacgcgc ttcgccgtcg
      601 cgcgggttca acatgatgac ggagcggagt tgctctgcgg cgtctgcgtg gcggttcatg
      661 agaaagagcg tgtaagcgta gttgtagagc acggaagcgt cttccgggtc agactctttc
      721 gcgcgttcga ggaaggtctg cgcttgcttg agcaggcgtt cgcgttcctc cgtggacttc
      781 tctgcgagcg cggcctgaat ggagagcgcg cctgcgttgt tgtagatgcc cgtgagcggc
      841 atgtcggagg cgacgggcag cagcgcgccg agcgcggtct tgagatcgtt catgcgccag
      901 tagctcaagg cggcgtagaa ggaggcttcc gcgaagtgcg ggtcgccttt ttgcaggcgc
      961 gtgaagtttt cggcggcgcg cttccagtct tcctgtttga agtagagcgt gccgagttcg
     1021 aaagcggcct gcgggtagct cgcgcccgcg ttcgtcttcg catactcctt gagcgcgttc
     1081 tgcaagtagg ccgaacgctt ctcgcggtct tcggtcatca cgcccttgac gtagctctcg
     1141 aaagcgcggg gcggaatctt cgtcgcgcgc tcgacgatct cgttgcgcga ataggcgagc
     1201 gtggaatcgc gttgatggag aatttgatag gcgaggatgc cctgcatctt ctgtaggttg
     1261 acgagcgcgt cgccgaaaat gtagggcgac cagatctgtc cttcgttgac gcgcacgaca
     1321 cgcgcgctga cgcgcacttc cgtcggcgtc ttgccgtctg ccggagtcac cacgtcgtaa
     1381 gtgccgatga cggcgagcgt cgctttcgct ttcatggcga cgttgatggc ggtggcgcgc
     1441 gagggcgtcg cggtcagagg caggcgcagg cgttgataga tcaactcgcg ctcgtccgtg
     1501 gagatggtgc gcagcccgta catctgcaag aggtcggtca gcgagtcggc aaaactcgtc
     1561 ccgatccagt gatactcgcg ctggctcgtc gtattctcaa acggcaggac gatgatcacg
     1621 tcgccgccct gcgccgcctg cgcgcgcacg gcaaacggcg cggctgaaaa gagcagcgcg
     1681 agcgtgcaaa ccgagagcaa agtcaaactt cttctaaaca tagttttatt cctgaatttg
     1741 taatatgcgg ggacaactat aacagactcg gggggcaaca tttaaacatc aaaagacgca
     1801 ccaaagataa acaagacgcc gaaaacttga gcaaaaccgc cgtgagcgca cgcgccgaag
     1861 cgtccgacga gagttagacg cgggagcgcg cggaacggtt ggcaggcgtg agggaacagg
     1921 aggctgctcg aaatgatact tcagggagcc tcttttattt tggccgcgca cgcaaattct
     1981 cacgccgcta aaagatgcgg cgcgtgaaga cgttggcgaa ttttccctgc gggtcttgct
     2041 gttgcctcag ttgttggaag cggacaaagt tgttctgata caagttcatc agatcatttt
     2101 tgtcgaagag ggtgcagaac tttccgaggt gcgggcgtgc gcccagactc ttcgcagagg
     2161 cttcggctgc cccgtagaag ttttcgaacc cgtaggaatc gttaatcacg aggtcgatcc
     2221 aggtgcgggg gccttgctgt cccgccccga ttaacgacca cgtatggtct ttcggggtga
     2281 agcgaatttc gaagagcgcg tagggcgtgt tcgaggtgta ggtctgttcg taaagctgga
     2341 tgaagtggtt gcacatcgcc agcgtgtctt cgaagggcac ggtgaattcc agctcttgat
     2401 ggaggtggta gatggtgcgc gtgtaggcct cgctgctttc cagcacgagg tctgtgccgc
     2461 gcttgaaggc gtaggagagg tttgaccagc gcggcaaaag tcccgtgtag gccatgaagt
     2521 ttccgaacca tgccgagagc agcgcgtccg ccgagatgct gatgagttca cgcagcgtgc
     2581 cgagcggcga cttgggcttg ttcgtgcgat tccaggtgtt gatctggcac ttgtctgtaa
     2641 agggaaagag gtagaggctg aagtgctcgt tctcctgtag gagattttgg aagttgaccg
     2701 ggtcgaagac gagcgttctg gttgacatgc gaaaattctg ctcgacgttg aagcgttcga
     2761 cggcctgcac gaccacctcc gagatgatgc cgaccgcccc cacgccgccg atggccgccc
     2821 ggaaaagatc gtccgccgga tggcacacct gcacatttcc cgcgccgtcg atgatgcgca
     2881 ggctgaccac cgattcgctg acgaagcccc aatccttgcc cgtgccatgc acgtccgttg
     2941 agaggattcc ggcgatgctc tgcgcgtcgt gagaaggcag agccttgaaa gcccagcctt
     3001 cgtccagcaa caacttgatg acatcgcgca tgcgcgtccc gcctttgacg cacatgcgtt
     3061 tcaagttcgc atccttccag accatcccgc tgtagttgtc gagcgagacg agcgtttcat
     3121 ctgcgaccac gccgtcgttg aaggaatgtc ccgcgccgaa gaacctgatg ccgctctcgt
     3181 tcttcacgat gtcgatgatc tcttgttcgc tcgttggtcg ccggaaacgc cccggacggt
     3241 agcgaaagtt tcgcgcccag ttgcggaaga agcctctgcg cacgcgccct tcgagccaga
     3301 ggaaggtttc aaacgtcagc gcgtagagga tgatgttgag ggtcgtcgcc agcgcgcatc
     3361 taaaatagcc tctaagtttc atggctcttt cctttcaaat gagaggtgcg aattgttgag
     3421 ataagctaga actcgaatcg tgagtctgcc tgtcaagaag cgttcgtgag gctggcgcga
     3481 gatggtgtgc ccgtcagccg gaaagtaagg agtaaacgcg ggcaagatat gcctgaatgc
     3541 aggcgttgtc aatagtgagc cgggtcgcac cccaaccgcc cggccccggc ccgaaggatg
     3601 ataaactgtg tcactgcgag gatgaattcg tctatgaaaa ggccgtcagc acggggagcg
     3661 gcaggctctt gaatttcgcg gagtagaggc gtgtccgaaa acagcaacag ccgacggaag
     3721 catcggctgt caaacatgaa gtaggcggtt cttcttcgtg aagcaaaaag tggcgtcccg
     3781 tacgggagtc gaacccgtgt cg
//
LOCUS       DASWNS010000441         7920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_485413, whole genome shotgun sequence.
ACCESSION   DASWNS010000441 DASWNS010000000
VERSION     DASWNS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485413"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..752
                     /gene="groES"
                     /locus_tag="VGB76_15145"
     CDS             426..752
                     /gene="groES"
                     /locus_tag="VGB76_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY0100285.1"
                     /translation="MNIRPLQDRVIVKRIEEGEQVRGGIIIPDSAKEKPQEGEVIAVG
                     EGKYKDDGTRQTLDVQVGDRVLFGKYSGSEIKLDGDEFLIMREDDILGIIARAGAQAG
                     GKKGSK"
     gene            870..2525
                     /gene="groL"
                     /locus_tag="VGB76_15150"
     CDS             870..2525
                     /gene="groL"
                     /locus_tag="VGB76_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0100286.1"
                     /translation="MAKQVIHGEESRAAILRGVNQLADAVKITLGPKGRNVVLDKKFG
                     SPTITKDGVTVAKEIELKDSLENMGAQMVREVASKTSDIAGDGTTTATVLAQAIFREG
                     VKTVAAGANPMALKRGIDKAVKRATEEIVRLAKPVKGDMIAQVGTISANGDRTIGELI
                     AEAMNKVGKDGVITVEESRTMETALEVVEGMQFDRGYLSPYFVTDPERMEAVLDNPFI
                     LINEKKISSMKDLLPLLEQVAKMGKPLLIIAEDVEGEALATLVVNKLRGTLNVAAVKA
                     PGFGDRRKAMLEDIAILTGGKVISEDLGIKLENVQVSDLGRAKKVTIDKDNTTVVEGA
                     GKDSDITGRVKTLKAQIEDSTSDYDREKMQERLAKLVGGVAVIRVGAATETELKEKKA
                     RVEDAMHATRAAVEEGIVPGGGVALVRAAKALEKFRANEEGEGDADEQIGVTIVKRAL
                     EEPLRQIVQNAGKEGAVIVEKVRESKKDSYGFNAATETFEDLVESGVIDPAKVTRSAL
                     QNAASIAGLMLTTEALVSELPDDDKAPAMGGGGMGGMGGGMGM"
     gene            complement(2643..2984)
                     /locus_tag="VGB76_15155"
     CDS             complement(2643..2984)
                     /locus_tag="VGB76_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100287.1"
                     /translation="MRKDNSTMRAPARPRREPDFDEPIWRWSTITITEERACELVDAF
                     TRYDDGDMIDALIQLICGLAPPPTIDEQQDLDERRLAGTAALARAFTYTRRAHNELGA
                     YVVGLKCAEWE"
     gene            3176..3403
                     /locus_tag="VGB76_15160"
     CDS             3176..3403
                     /locus_tag="VGB76_15160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0100288.1"
                     /translation="MGLISTSEAAEKLGVHITRVQVLIREGRLPAQKIGRTYVIDEDN
                     LKLVKDRKAGRPPKAKAEIDSKASKKRGGKK"
     gene            3438..4337
                     /locus_tag="VGB76_15165"
     CDS             3438..4337
                     /locus_tag="VGB76_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100289.1"
                     /translation="MREGAFALIDCLGFKGIWRRTDPQLLLKKLLEVEEAVRQQVAER
                     EELKFFKFSPVEARIRLLSDTVAISLQYKHKEDLIPSESDKNWLVMIMCLLVVKILDI
                     FITDEPPLVLRGCISYGEHISEGNFIVGQAVDDVAEHMNIAEGAFVWLLPKAATRYES
                     HRERMNELLASISPEAVKPAIEYLGVRNYHPPSVNEWGSEAHREEIVARIRYSQSLPI
                     VISDYEMPLKSGARLRCAVLNPLSFYTSSEKRKEIIEQYSKVISGNQIDIWLKHQHTM
                     DLLEVADQICSSFRDTHVEPETN"
     gene            complement(4487..6004)
                     /locus_tag="VGB76_15170"
     CDS             complement(4487..6004)
                     /locus_tag="VGB76_15170"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0100290.1"
                     /translation="MKNSNVIMRLLLSLVLSLSTSTGSLAVAQQQRAAAPVERAQAGG
                     SSATKTTAAAPAPPQVLERNVRAHLEFLASDAMQGRGSGTQFELLAGHYIASQLRQYG
                     IEPAGDADASAAGQKSFIQTVKLTRQAFADAPALSFSAAGGAERRWVHGREMLVGRVA
                     AAQVRGALQKLRAGEKPAPGAVVLLTQGAGDARQLSSEAAAMGQGGAAAVIVAENAML
                     RRNWAAMGARLPELPLMTGAGRSASTILVLSTDAFKELQAVADGTIINFGGTLAPAQT
                     NYTWNVVGVLPGSDAKLSNEAVLLSAHMDHIGVGTGQTGDQIYNGADDDASGVVAVLE
                     LARALAAGGAGTRPKRTTYFVLFGSEERGGYGAQYFLAYPPVVLTQMVANLEFEMIGR
                     PDAKVAADTLWLTGYERSNLGAELARQGARLVADPHPEQNFFQRSDNYALARRGVVAH
                     TVSSFGLHPEYHQPSDDITHIDFPHMTRAINSMVAPVLWLLNSNFTPTWVEGKKP"
     gene            complement(6213..6716)
                     /locus_tag="VGB76_15175"
     CDS             complement(6213..6716)
                     /locus_tag="VGB76_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100291.1"
                     /translation="MSDLLTAISILIATITWLLTYFQDRSARRVAHTADIIAGISTSD
                     RLAESSFQVTKLINSGTKVSLSEIDPKTESHVVDILDYYEFLCDLYASHVLSRRTIVD
                     LRGRLMKRTWDICETYIKETRKLQNRMVYLGFEKFVKELPPEVSTPLIAISDSTSDST
                     RKKVTTS"
     gene            complement(7016..7546)
                     /locus_tag="VGB76_15180"
     CDS             complement(7016..7546)
                     /locus_tag="VGB76_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100292.1"
                     /translation="MKNLSGCATLLALIFFVLPFKLHAQSGAPRPSESPSTISADQEI
                     IETRPIDIKGCIRDSRAGQSVIRDAESFQQAIRADASREWCRKNLESIDFTKHSLLGI
                     TLMTDYCDRPRGLAHQLIKDTSTKKYLFDISYHTPLAVCRRMGYWDVWVVVPRIPDDY
                     EIVFKVRKIPLNETEQ"
     gene            complement(7727..7801)
                     /locus_tag="VGB76_15185"
     tRNA            complement(7727..7801)
                     /locus_tag="VGB76_15185"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7767..7769),aa:Gly,seq:ccc)
BASE COUNT         1889 a         2092 c         2256 g         1683 t
ORIGIN      
        1 acctctcacg caccgcagcc acgctcggca tccaccgcaa cacgctctcc aaacgcgtcg
       61 ccgcctaccg cacacaggaa cgcgccagca gcaaaaccgc cgcacgccgc tctcgctagg
      121 aaatcgaaga gaccgaaaaa cccggctttt tcgcgcgtcc cgccaaaatc tgagtggtct
      181 acactcacat ttttatccac ccatattgac aagtgccgtc cgctcgctat attcttagca
      241 ctcgcttcca gagagtgcta gaaaatttgc ccggatgaac tgttaattgt tttgttttga
      301 ggcgcaagcg agtcggcgtt ctgaatatgg agaggcgaga aaaaagttct accagtctgt
      361 tttttgcaat caaacacgct gaaaaattca acgttgaaat ctgaaaggag taattcttca
      421 tggcgataaa catcagacct ctacaagacc gcgtcattgt gaagcggatc gaggaaggcg
      481 agcaggtacg cggcgggatc atcatccccg acagcgcgaa agagaaaccg caggaaggcg
      541 aagtgatcgc cgtcggcgaa ggcaagtata aggacgacgg gacgcgccag actctcgacg
      601 tgcaggtggg cgaccgggtt ctgttcggca aatacagcgg ctcggagatc aagctcgacg
      661 gcgatgagtt tctgatcatg cgcgaggacg acattctcgg catcatcgca cgcgcgggcg
      721 cacaggctgg cggcaaaaaa ggcagcaagt agttctcgac gactgagaac gctgctgcgc
      781 ttttgcataa cgcttacgct ttgtgcataa cgctcacgct tttgtgtgtt gactgaaaga
      841 ttgagaatca atttgaggag aataggttta tggcgaaaca agtaattcat ggcgaggagt
      901 cgcgcgcggc gattttgcgc ggcgtcaacc agcttgccga cgcggtcaag atcacactcg
      961 gccccaaagg gcgcaacgtc gtgctcgaca aaaaattcgg ctcgcccacg atcaccaaag
     1021 acggtgtgac ggtggcgaaa gagatcgagt tgaaagactc tctggaaaac atgggcgcgc
     1081 agatggtgcg cgaagtggcc tcgaagacct ccgacatcgc gggcgacggc acgacgacgg
     1141 cgaccgttct ggcgcaggcg atcttccgcg aaggcgtgaa gacggtggcg gcgggcgcga
     1201 acccgatggc cttgaagcgc ggcatcgaca aggccgtgaa gcgcgcgacg gaagagatcg
     1261 tgcgtctggc gaagccggtg aagggcgaca tgatcgcgca ggtcgggacg atctctgcga
     1321 acggcgaccg caccatcggc gagttgatcg cggaagcgat gaacaaggtc ggcaaagacg
     1381 gcgtcatcac ggttgaggaa tcgcgcacga tggagacggc tttggaagtc gtcgaaggca
     1441 tgcagtttga tcgcggctac ttgtcgccct acttcgtgac cgaccctgag cgcatggaag
     1501 cggtgctcga caacccgttc atcctgatca acgagaagaa gatcagttcg atgaaagatt
     1561 tgctgcccct gctcgaacag gtggcgaaga tgggcaagcc gctcctcatc atcgcggaag
     1621 acgtggaagg cgaagctctc gccacgctcg tcgtcaacaa gctgcgcggc acgctgaacg
     1681 tcgcagcggt caaagcgccg ggcttcggtg atcgtcgcaa ggcgatgctc gaagacatcg
     1741 cgattttgac gggcggcaaa gtgatctcgg aagacctcgg catcaagttg gagaacgtgc
     1801 aggtcagcga tctcggacgc gccaagaagg tgacgattga caaggacaac accacggtcg
     1861 tcgaaggcgc gggcaaagac tctgacatca cgggtcgcgt gaagaccttg aaggcgcaga
     1921 tcgaagacag cacttcggac tacgaccgcg agaagatgca ggaacgtctg gcgaaactcg
     1981 tgggcggcgt cgcggtgatt cgcgtcggcg cggcgaccga gacggaactc aaagagaaga
     2041 aggctcgcgt ggaagatgcg atgcacgcca cacgcgcagc ggttgaagaa ggcatcgtgc
     2101 cgggcggcgg cgtcgcgctc gtgcgcgcgg cgaaggcgct tgagaagttc cgcgccaacg
     2161 aagagggtga aggcgacgca gacgaacaga tcggcgtcac catcgtcaaa cgcgcactcg
     2221 aagagccgct gcgccagatc gtgcagaacg ctggcaaaga gggcgcggtc atcgtggaga
     2281 aggtgcgcga gtcgaagaag gactcctacg gcttcaacgc tgcgaccgag acgtttgaag
     2341 acctcgtcga gtcgggcgtc atcgaccccg cgaaggtgac acgcagcgcg ctgcaaaacg
     2401 cggcctccat cgccggattg atgctcacga ccgaagctct cgtctccgag ttgccggacg
     2461 acgacaaagc ccccgcgatg ggtggcggcg gcatgggcgg catgggcggc ggcatgggca
     2521 tgtaatcaaa gcccgtccca accggacaat gcaaagcggc ttcgttccac gcgaacgagg
     2581 ccgctttcgt ttgcagcggg cgactgcgga gactcactag cgcacgcgct agtgagtctc
     2641 ccctattccc actcggcgca cttcaacccg acgacatacg cgccgagttc gttgtgcgcc
     2701 cgccgtgtgt aagtgaaggc tcgcgcgagc gcagccgttc ccgccaaacg ccgctcatct
     2761 aagtcctgct gctcgtcaat cgtgggcggt ggcgcaagtc cacagatcaa ttgaattagc
     2821 gcatcaatca tgtcgccatc gtcatatcgt gtgaacgcat caaccaactc acacgcccgc
     2881 tcctcggtga tagtgatggt tgaccaacgc cagatgggtt catcaaaatc cggctctcgt
     2941 cgcgggcgag cgggtgctct catcgtgcta ttatcttttc gcatcagtat ctccttcaaa
     3001 agtcatactg gtgaagaggg cttacctaag attcatggtc ttggtaagtc ctcgcctatt
     3061 tgtgctctca tcagtctatc gagtgcgcaa tagtatctgc ccaagcagat agttttgtca
     3121 atagaaatcc ctctttattg acagatattt ttatggattg tataaggcta aacttatggg
     3181 gctgatttct acatctgagg cggcagaaaa attaggcgta catataacgc gcgtgcaagt
     3241 gcttatcaga gaaggtcgct tgccagcgca aaaaataggg cgcacttatg tgattgatga
     3301 agacaacttg aaattggtta aagatcgtaa agcaggcaga ccaccaaagg ctaaagctga
     3361 aatagattca aaggcgagta agaagagagg cggtaagaag taagtgaggg tttcagcaca
     3421 acaaaatagg aggttaaatg cgagaaggag catttgcatt aattgactgt cttgggttta
     3481 aaggtatttg gaggagaacc gatccgcaac ttttacttaa aaagctgtta gaggtagaag
     3541 aggccgtcag acagcaagtt gctgaaagag aagaactcaa attctttaaa tttagtcctg
     3601 tggaagcgcg tattcgttta ctgtcggata ctgtggccat cagcttgcag tataagcata
     3661 aggaagattt gatccctagt gaatcagata aaaactggtt agtaatgata atgtgtctgt
     3721 tagtagtaaa aatccttgat atctttataa cggacgagcc tcctcttgta cttagaggct
     3781 gtatttcata tggtgaacac ataagtgaag gtaatttcat tgttggtcaa gcagtggatg
     3841 atgttgcgga gcacatgaat atagcagaag gtgcatttgt ctggctgtta cccaaagcag
     3901 ccacaagata tgaatcgcac cgcgagagaa tgaatgagct acttgcaagc atatcacctg
     3961 aggcagtaaa accagccatt gagtatttag gcgtgcgcaa ctaccaccca ccatcagtta
     4021 atgaatgggg ttctgaggca catagagaag aaattgtagc tagaatccgc tattcacaat
     4081 ctcttcccat tgttattagt gactatgaaa tgccgcttaa gtcaggagcg cgtttacggt
     4141 gtgcggttct taatccgctg tcgttttata catcttcaga aaagcgtaaa gaaatcatcg
     4201 aacaatattc aaaagtaatt tcagggaatc aaatagatat ttggttgaag catcaacata
     4261 caatggattt gcttgaagta gcggatcaaa tatgtagttc tttcagagac actcatgtcg
     4321 aaccggaaac taactaacaa gtagattatc ggctctggca attttaaaca aaacgtacgc
     4381 aggtctatcc acaataccaa cctttataag catcataaaa tgcagtgcat tggtataagt
     4441 agctatagtt taactcactt ctcgtctcta taacgggctt tcaacatcat ggcttcttgc
     4501 cttccaccca cgtgggggtg aagtttgagt tgaggagcca gaggacgggg gcgaccatgg
     4561 agttgatggc gcgggtcatg tgtgggaagt ctatgtgggt gatgtcgtcg gagggttggt
     4621 ggtattcggg gtggaggccg aagctcgaaa cggtgtgggc gacgacgccg cgacgcgcga
     4681 gggcgtagtt gtccgagcgt tggaagaagt tctgttcggg gtgggggtcg gcgacgagtc
     4741 gtgcgccttg ccgtgcgagt tccgcgccga ggttggaacg ctcgtagcct gtgagccaga
     4801 gcgtgtcggc ggccactttc gcgtcgggtc gcccgatcat ctcgaactct aagttggcaa
     4861 ccatctgcgt gagcacgacg ggcgggtatg cgaggaaata ttgcgcgccg tagccgccgc
     4921 gctcttcgct gccgaagagt acgaaatagg tcgtgcgctt cggccttgtg cccgcgccgc
     4981 ctgcggcgag cgcacgcgcc agttccagca cggcgacgac gcccgaagca tcgtcgtccg
     5041 cgccgttata aatctggtcg cccgtctgcc ccgtgcccac gccgatgtgg tccatgtgcg
     5101 cgctcaagag cacggcttcg ttcgacagtt tcgcgtcgct tccgggcagc acgccgacta
     5161 cgttccacgt ataattcgtc tgtgcgggcg caagcgtccc tccgaagttg atgatcgtgc
     5221 catcggcgac ggcttgcagt tccttgaagg cgtccgtgct caggacgagg atcgtgcttg
     5281 cgctgcgtcc cgcccctgtc atcaacggca actcgggcag cctcgctccc atcgcagccc
     5341 agtttcgcct gagcatggcg ttctccgcga cgatgaccgc cgccgcgcct ccctgcccca
     5401 tcgccgccgc ctccgaagac aactgccgcg cgtcgcccgc tccctgcgtc aacagcacca
     5461 cagcccccgg ggcgggcttc tcccccgcgc gcaacttctg caaagcccct ctcacctgcg
     5521 ccgctgcaac gcgcccgacg agcatttccc gcccgtgcac ccaacgccgc tccgcgccgc
     5581 ccgccgcgct gaaactgagc gcgggcgcat ctgcaaaagc ctgccgcgtt aacttgacgg
     5641 tctgaataaa actcttctgc cccgccgccg aagcatccgc atcgcccgca ggctcaatgc
     5701 cgtactgtcg caactgcgaa gcgatgtagt gtcccgccaa caactcgaac tgcgtcccgc
     5761 tcccccgccc ctgcatcgca tcgctcgcca gaaattccag atgcgcgcgc acgtttcgct
     5821 ccaacacctg cggcggcgca ggagcagcag cagtcgtctt cgtcgcactg cttccgccag
     5881 cctgcgcgcg ttcaaccggc gccgctgcgc gctgttgttg cgcgacggcc aaactgcccg
     5941 tcgaagtcga gagagagagc acgagcgaca atagcagccg catgatcaca tttgaatttt
     6001 tcattccgcg atgcgccccg tgggattttg gtcaattatg aatttacgtg tcgaacgagt
     6061 cctgttgtat cacaggctct cgtcgatacg taaagagatc acgcgatact tatcaaacat
     6121 atgcgcgcag cgcaaaatga aagagacgac gcccgctcaa agcgtcgtct cttttctatc
     6181 cggaggcgaa agccgtagag agcgacggga gtttaggatg tcgtcacttt tttacgggtt
     6241 gaatcacttg ttgaatcact aatcgcaatt aagggagtgc tgacctcggg cggtagttcc
     6301 tttacgaatt tttcaaaacc cagatagacc atgcggtttt ggagctttcg cgtctccttg
     6361 atgtatgtct cgcagatgtc ccatgtgcgc ttcatgagcc gcccgcgcaa gtccactatc
     6421 gtccgcctac tcaggacatg gcttgcgtac aggtcacaaa ggaattcgta gtaatccaga
     6481 atgtctacga catgcgactc ggttttaggg tcgatctcac tgagcgaaac tttggtcccg
     6541 gagttaatca gtttggtgac ctgaaagctc gactccgcta accggtcgct ggttgagatg
     6601 cccgcgataa tgtctgcggt gtgtgccacc cgccgggctg agcggtcttg gaagtaagtc
     6661 aggagccagg tgatggtggc aatgagaatt gagattgcag tgagcagatc gctcatattc
     6721 cagacctcca tattgaacag tcgcaggcgt ccggcgatct acttaatatc gcgccggtga
     6781 gacgcttatt tgcagcctgc cgcctgccat cagccgccgc ccatttcaac atgtctttca
     6841 agtggacgtg agattaacgg aggcttatca gaggttaagc ggagggaaga tataagggcg
     6901 cggggtgagt gtcaatagtc agaggtattg aatatgtgtc ctcagagtgg ctacgcattc
     6961 atcccccggc agagatgcag tggtcgtctc cgcgctgcca aatgattctc ttaatctatt
     7021 gctcagtttc attcaaaggt atttttctga ctttgaagac gatctcataa tcgtctggaa
     7081 tcctggggac aaccacccac acatcccaat aacccatccg gcggcagacg gccagtggcg
     7141 tgtgataact gatgtcgaaa agatatttct tagtagacgt atcctttatg agttgatgag
     7201 caagcccccg cgggcggtcg caataatccg tcatgagtgt gatgccaagc agggagtgtt
     7261 ttgtgaaatc aatgctttcg agatttttgc gacaccattc ccggctggca tctgcgcgga
     7321 ttgcttgctg gaaagattca gcgtccctga taacgctctg tcctgcgcga gagtctctga
     7381 tacagccttt aatatctatt gggcgagttt cgataatttc ctgatcggct gatattgttg
     7441 atggggattc ggacggtcgg ggcgcgccgc tttgagcgtg cagcttgaag ggcaagacga
     7501 agaagattaa agccagcagg gtggcgcagc cagagagatt tttcatgcgc cgcagagtag
     7561 caaaaactct taagcaggga aagttcagcc ccccggatag accccgccta aatttagact
     7621 tctctgtaga ttattaccaa tagaagagcc tattacgtaa gagggttgcg gatacgggta
     7681 taaacaaaac ggacggcatg atgccgcccg tagctcgtcg gtattatgga gcgggcgacg
     7741 agactcgaac tcgcaaccct cagcttggga agctgatgcg ctaccattgc gccacacccg
     7801 ctcaaagaaa atcgtcgatg ccttccgcgc gtcaagcatc cggggtgaga tggaagatac
     7861 tcaaacgaat gcgtggctgt caaacccggt ctgacgcgga gactgtaacg cgcagactct
//
LOCUS       DASWNS010000442        18850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_226637, whole genome shotgun sequence.
ACCESSION   DASWNS010000442 DASWNS010000000
VERSION     DASWNS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18850
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226637"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="folK"
                     /locus_tag="VGB76_15190"
     CDS             <1..399
                     /gene="folK"
                     /locus_tag="VGB76_15190"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY0100293.1"
                     /translation="GVSVAQPLFLNMVAELSFADTDHAQSVAAGAPPPSPEQLLARLL
                     RIEYALGRRRAQPLAPRTIDLDLLLYGDACSDTEYLVLPHPRLHLRRFVLVPLSELTP
                     EAAHPKFGQTFGQLLAALRDDSSVRVWRPK"
     gene            complement(486..1502)
                     /locus_tag="VGB76_15195"
     CDS             complement(486..1502)
                     /locus_tag="VGB76_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100294.1"
                     /translation="MPGEPEAHDRIIYGQYPTLPGEIATPHPDQPPIPPGETVDMVLK
                     DYEGIRDFLNNTHYPASINRLEISVGDVVFDDGTKWSGGGIFRRDPDKPGGWIRIKEQ
                     VPTSQDRNDVSYGLLTSDSKKHAFARSNAEVVPQFLKADEDSPLTEPGSTPQMCCDYS
                     PIIIDVAGDGFRFTDAAGGVDFDFNGDGVRHRMSWTAANSDDAWLALDRNANGTIDHS
                     AELFGNLTPQPSVARPQGFLALAEFDRPQHGGNADGLIDTRDAVFTRLRLWQDANHNG
                     LSESNELRPLPALDVTALGLDYHESKRTDEHGNPFRYRAKVTDTKGAKVNRWAWDVYL
                     VAAP"
     gene            complement(1986..3149)
                     /locus_tag="VGB76_15200"
     CDS             complement(1986..3149)
                     /locus_tag="VGB76_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100295.1"
                     /translation="MPAIALLAFFLVLGGIGNVSSTAQSSPEARELEDKIPKHLPIKV
                     KIKNLDKENWVREVEVEVKNTGDKPIYYLRFSLRMPEVLSETGSVVAVSFSYGRPAIG
                     DFRNRPTAEDVPIQPGETYVMKVPENEMRGWESARKHYKWSEPKKFLIKFRKLHYGDG
                     TGYGTSGGLPVPNPNATSSSCGGSRRQTAPAQSASSGGGLKPPLAPSFQFAPSLLPVR
                     FLPAKLFVERTVEPIPNFASPQAGVCCPGIYGCFSGREVEEGHTCVTCGPAAWVESAT
                     CGSPGSYCGKIEDETKFCDDADGNRVGCTYYSLDPCPPPPPDTTECGTGFAVPCPTDE
                     ESPFTEPGSTPQMCCDYSPIIIDVAGDGFRFTDAAGGVDFDFNGEDGVRHRLS"
     gene            complement(3275..3961)
                     /locus_tag="VGB76_15205"
     CDS             complement(3275..3961)
                     /locus_tag="VGB76_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100296.1"
                     /translation="MHTHTILSRLRNLKLTMLAAILALCAVGVVPGTAQSSPEARELE
                     DKIPKHLPIKVKIKNLDKENWVREVEVEVKNTGDKPIYYLGFNLQMPEVITETGSVVA
                     VTFRYGRPELGRFESRPTAEDVPIQPGETYVMKVPENDMAGWEAARDNRKWSEPKKFR
                     LKFYLLHHGDGTGFMTSGGLPVPNPNATSSSCGGRGRQTAPAQNFDFNGDGVRHRMSW
                     RAMSAGKDVS"
     gene            4202..5104
                     /gene="panB"
                     /locus_tag="VGB76_15210"
     CDS             4202..5104
                     /gene="panB"
                     /locus_tag="VGB76_15210"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY0100297.1"
                     /translation="MAYLKQQRPEKVTVPSLRASKERKERLVCLTAYDYPTARIVDEA
                     GTDIILVGDSVGNVVLGYDTTVPVTLKEMVRHTRAVRRGVERALLVADMPYGSYHTGE
                     DDAVRAALRLIKEGGAEAVKLEGGRAREGVVRRLVAEEIPVMGHIGLTPQSVNKLGGF
                     RLQGKTAEAARALVEDARALEAAGAFALVLEVVPREIARVVTASVRIPTIGIGAGEHC
                     DAQVLVIHDLLGLSFSPTRPRFVRQYADLRAQMTEAISRFAEDVRTGAYPAENESYPL
                     PREAAAELEATPISQSGDAETERP"
     gene            5208..6089
                     /gene="panC"
                     /locus_tag="VGB76_15215"
     CDS             5208..6089
                     /gene="panC"
                     /locus_tag="VGB76_15215"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY0100298.1"
                     /translation="MEIINRRQRMNSVSRKVRRELDKTIGLVPTMGALHEGHLSLVRE
                     ARRMCDVVVVSVFVNPKQFGPQEDFSRYPRDLTKDTMALADYNVDYIFAPPPEEIYPK
                     GFSTYVEVERLSEPLEGAARPGHFRGVATVLTVLFNIIRPDFAYFGQKDAQQTLVVKR
                     LVRDLAFDTEIVILPTVREDSGLALSSRNAYLEPEDKRAAAIIYRALAHAEAAYGEGE
                     RSGKKLADIVRADIEAEPRATLEYVSVNDADTLERLDRLDDRPVLIAVAARLGKTRLI
                     DNIVLNKMSKTGQLVMP"
     gene            6226..6756
                     /locus_tag="VGB76_15220"
     CDS             6226..6756
                     /locus_tag="VGB76_15220"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0100299.1"
                     /translation="MPERTKHKAHIEFTLNGEAVEVAFAPHKTLLEVLREDLNLMGTK
                     HGCELGECGTCAVLVNGRSVLSCLVLGLDAEGRSVETVEGMASPAGLHPLQETFADTG
                     AAQCGYCTPGFLLVAKELLAVNPKPTREEIKEALSGNLCRCTGYIKIYEAVELAAARM
                     RGEELELPREAVYGLE"
     gene            6833..10000
                     /locus_tag="VGB76_15225"
     CDS             6833..10000
                     /locus_tag="VGB76_15225"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEY0100300.1"
                     /translation="MPNKISQHIDNFRQKVEVANKEATKKEAFKDLLNRLYADDEETR
                     KVVDAISGGAERAVVNIPRKGKRHRGSADTLYNRTIIEFENDLRKSLAHGKEQLAGYL
                     LGEINSGETYNHTLIASDFINWKVYAPDVSQLAMLETVNEDELKLKEVESTSFTLTEH
                     NAEEFYYWIDRYLFREKKQRATLKRIAESFGYQSPVFIQCFRDLTKSFRAVKASGEVQ
                     VSFEQWEKFLSIAYGSFVASEQNFLIHTYLSVFAKLLAYAVVSNNDIDEEKLREAIEG
                     SLFDQFNITNFVENDFYHWVASAEIFPALKESFRGITQEFATYDFQDVDEDVLKGVYQ
                     EMIDLDTRHALGEYYTPDWLCERVVAEFKFQPTDKILDPSCGSGSFLRAVIGRIRKLH
                     PRVTVEDLNEQIYGIDIHPLSVQIAKTTLLLAYGRAVVNAKKPVHLNIILANTLLLPD
                     EVSDLFGKHFTVKIDRKDYELDVGVLDDLRVFDEALNLSDDLAEQTRGKGAQALSDFR
                     NALEQQAGAGELSEEVAESFHRIYQGFKRAKEDGRDSIWKFIVQNLYKPHFLAAKIDY
                     IVGNPPWFTYSDINNEDYQVLLNKLAVKHDVKPERRANFPHLEIAAIFLAYCSGFFLR
                     AGGKIAFVLPRSFFSADQHDNTRSGKAKGFALTGAWDLKDVSPLFRVPTCVLIGEQRA
                     EAATAVTVSIEGRTFEGKLPAHNCNLKAAAPELTEASVRWFYAKQGRSTAFSMRQPDA
                     RNTTNPYKKNFKQGATIVPRAFYFVDINQELPPDFEERLINVRTAASIQPEAKKPWTE
                     ITLKGRIESRFLFRTALSKSILPFALFAPNLVALPITVKQNTSGSKQLEMLSAEQLRK
                     DGFLHAAKWFTEVERLWEERKTDKSRNMTNLGRVNYQGGLTGQNLDAPYLVLYNSSAQ
                     DANATVVKRTDFDLEFIVESVAYVFYTYALDEAYYLAAIFNSSVPNEKMKDFQARGLF
                     GARHVHKKILDVYFPRFVAGDDKHARLARLSETAHKKANAFLLANPPAGALTPRRLGR
                     LRVAVKTELAAELSAIDESVKEIIA"
     gene            10060..12489
                     /locus_tag="VGB76_15230"
     CDS             10060..12489
                     /locus_tag="VGB76_15230"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0100301.1"
                     /translation="MSQNKDSKDFKVVGRPFRKVDARAKCAGQTKFADDIVLPRMLFA
                     KLLRTHEPHARIVSIDISKAVALPGVHAVITGKDLPTTYGILPVSQDEHALCVDRVRY
                     IGDPVAAVAAVDEDTAFAAMNLIEVEYEKLQTITSIEEAIMIDEPRVQDYGESGNVHK
                     KVHLEFGDMDEGFAEADHIREDTFFYEGNTHLPMEQHAAVAHFDPDNKLTLWSSTQTP
                     HYVHRALTKVLGLPAAHIRVIATPNGGGFGGKSDPFNHEVAVCKLSMITGRPVKVTLT
                     REEVFYCHRGRHPVLMNVKTGFKRDGSITAMDFQTFLDGGAYGSYGVASTFYTGALQT
                     VTYEVPRYRFRGLRAFTNKPPCGPKRGHGTPQPRFGLEVQLDKIADDLKLDPAEMRLK
                     HLVKPYSLTANYLRIGSMGLGKCIEKIVEGSDWKNKFRKLPYGKGVGLACSSYICGAG
                     LPIYWNNMPHSGVQLGLDRQGGVRVQCGSTDIGQGSDSILAYIVAEVLGIDPFDIRVL
                     TADTDLTPVDLGSYSSRVTLMTGNAALQAAERARELLSRAVAEKLSLPVENLSFADHR
                     VFDVENPEVGVTFAEAVVLAESKYGTIGTVGSYTPPRSPGKFKGAGVGPSPAYSYSAA
                     IAEVDVDAETGFITVERIWIGHDIGQSINPMLVMGQVEGGVYMGLGEALMEEMIYRAN
                     RNVVHKFPSLLEYKSPTTHEMCDVKTYLIEDADPNGPFGAKEVGQGPLLPVMPAVANA
                     VYDAVGVRVDEVPITPEKVLAALRAKAQGKPARFGPKGVPVVEWPEPLKVATPAEGGD
                     GREMPRVAIHS"
     gene            12661..13197
                     /locus_tag="VGB76_15235"
     CDS             12661..13197
                     /locus_tag="VGB76_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100302.1"
                     /translation="MTLSAWPQTSSAQDKITTQLGDSAEGAAIFKTPKGYMQIKLEGF
                     RGVLILDPKKPAGMFISYPDDKETTESLRRRVRTRVTEMFIHGDKTPELDWSAKPLPP
                     HKGDGEGKASLETATSGETEIQLATYERTTGVRPFVYGYFAMRNKSGKGEKVHFIDEQ
                     GLGVKKFDELWQSMPGSK"
     gene            13312..14298
                     /locus_tag="VGB76_15240"
     CDS             13312..14298
                     /locus_tag="VGB76_15240"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY0100303.1"
                     /translation="MMRLPKFEYRVPREVRDAVKMMADAGAQGQFVAGGTDLYPNMKR
                     LQQTPKIVISVGRIAELNKIEGDGKSGVRVGAGVSLTDLCEHPVINRDYPMVAAAART
                     ISTPVLRNMGTIGGNLLLDTRCNYYNQNYEWRKGVNFCMKKDGDVCWVAPGSSKCWAV
                     QSADLVPLMVALGARVRFVSTLGEREVTAAGLYNDDGIDYLHKRPDELLADIQLPPLN
                     GWRAVYKKLRRRGAFDFPVLGVAARLDMEADGTVKDAKLILGGVAPAPLSVTEAQNAL
                     IGAPLDAERIRAAAEACYVKARPLDNTDFIMNWRKQMTRIYVVRALESLQGE"
     gene            14398..14844
                     /locus_tag="VGB76_15245"
     CDS             14398..14844
                     /locus_tag="VGB76_15245"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0100304.1"
                     /translation="MKKLILIIAALVSLSSAAFAQQGAEAEREAVKQAVLDYVEGIYT
                     VDASRIERSVHPELAKRGFFVKRGETAYSSDVMTFQQLVELAKTYNKNGRVPKEAPKE
                     VVIYDVSDQTASAKLTAVWGIDYIHLAKYDGKWKFVNVLWQTPPKK"
     gene            14957..15619
                     /locus_tag="VGB76_15250"
     CDS             14957..15619
                     /locus_tag="VGB76_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0100305.1"
                     /translation="MPVAALLLAAGRSRRMGAFKPLLPFGAQTVIETCINQLLAAGCE
                     EIVVVVGHRAEEVRARVSHLPVRFACNDEAGSEMGVSIARGVEELTAGDRAAAATTTT
                     PGAVLIALADHPAVTAQTIEEVIAAHRRTGARLVVPEWQGRGGHPVLVSMGLRASLLN
                     LDSVGGLRSLFAAHAGEVLRVPVASGYIARDMDTWEDYRTLHEEVFGVPPPVTLLSGQ
                     QT"
     gene            15784..16338
                     /locus_tag="VGB76_15255"
     CDS             15784..16338
                     /locus_tag="VGB76_15255"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0100306.1"
                     /translation="MFIKSPRFILSLVLSSMLVFAQALASTPAPVKTQEGLLDFSLRS
                     VDGGSITSEQLRGDVVILAFGASWLPLSRTQVEGVQRLADEYRERDLRVYWVSTDSES
                     QKSKNFATDDQLRSFARKNGLKIGVLRDPDGALFKRLGVDRNQLPALVIFDKEGQISG
                     APIGGLDPGGNLVQRLSARINALL"
     gene            complement(16432..17058)
                     /locus_tag="VGB76_15260"
     CDS             complement(16432..17058)
                     /locus_tag="VGB76_15260"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY0100307.1"
                     /translation="MENRGERQVEASAGGAGGFALEQLVLASGSPRRAEILRRTNWPF
                     EVEAADIDESAREGEGAVEYVERLACEKAEAVAAGRTKGLVLGADTVVVVAGQVLGKP
                     KDEADARRMLKLLDGRWHDVLSGVALVRAETNEVLVAHELTRVRFASMSDAEIDWYVS
                     TGEPADKAGAYAIQGHAALFIEEIEGDYWNIVGLPIRLVYKLAAESRS"
     gene            17188..17658
                     /locus_tag="VGB76_15265"
     CDS             17188..17658
                     /locus_tag="VGB76_15265"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEY0100308.1"
                     /translation="MDDLIRSLPNLLRAAGDAEEVAEAAAMVAWRRVAGEALRGQAVP
                     FRLHRKLLVVAVPDTAWQKQLESISGQLIFRLNSMLGQAVVTYIEFRVDPKTVRAERA
                     RLGQTEFDRAAHERRSLQNARQLTDAADAIQDEDLRRRFLLAAGSYIGAQQKNK"
     gene            17749..18045
                     /gene="gatC"
                     /locus_tag="VGB76_15270"
     CDS             17749..18045
                     /gene="gatC"
                     /locus_tag="VGB76_15270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY0100309.1"
                     /translation="MPITESDIEKIAALAQLEITDEERRALTPQIASIVTYVEQLNEL
                     DTSAAEPALGGLTPEGARTDTAREDAPLPSLGQSLALDQAPDAAAGHFRVPKVL"
     gene            18120..>18850
                     /locus_tag="VGB76_15275"
     CDS             18120..>18850
                     /locus_tag="VGB76_15275"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY0100310.1"
                     /translation="MSIENIRRRLARGEQDARAASETALNAAEELNETLHAFLQIDRA
                     GALARVDALDARRDESLNGALGAVPVAIKDNICVRGLQTSCGSAILGDYQPPYNATVI
                     ERLLDAGAIIVGKTNCDEFAMGSSNENSAFGAVRNPWDTKRVPGGSSGGSAAAVAARI
                     VPVALGSDTGGSVRQPASLCGIVGVKPTYGRVSRYGLVAFGSSLDQVGVFATNTHDAA
                     LTLSVIAGRDARDATTADMVVPDYVS"
BASE COUNT         3952 a         5826 c         5431 g         3641 t
ORIGIN      
        1 ggcgtctccg tcgcacaacc cctgttcctg aacatggtgg cggaactctc tttcgccgac
       61 acagaccacg cccagagcgt cgctgccggt gccccgcctc cttcgcccga acaactcctc
      121 gcgcgcctgc ttcgcatcga atacgcactc ggacgccgcc gcgcacaacc tctcgccccg
      181 cgcaccatcg atctcgacct cctgctctac ggcgacgcct gctccgacac ggaatatctc
      241 gtgctcccgc acccgcgcct gcacctgcgc cgcttcgtgc tcgtgccgct ctccgaactc
      301 acgcccgaag cagcgcaccc gaaattcgga cagacgttcg gacaacttct cgccgccctg
      361 cgtgacgact caagcgttcg agtctggcga ccgaaatgat ccgttgcgcc ttgagagagc
      421 aaaccgcccg caggttgtcg caaacctgcg ggcggctctc gttctctact gcttttctcc
      481 ggcgctcaag gcgcggcgac gaggtacacg tcccacgccc agcgattaac cttcgcgcct
      541 ttcgtgtccg tcaccttcgc gcgataccgg aaagggttgc cgtgttcgtc cgtccttttc
      601 gactcgtgat agtcgaggcc gagggcggtc acgtcgagcg cgggcagggg gcgcagttcg
      661 ttcgactcgg agaggccgtt gtgattcgcg tcttgccaga ggcgcaggcg tgtgaagact
      721 gcatcgcgcg tgtcgatcag gccatcggcg ttgcccccgt gttgaggccg gtcaaactcg
      781 gcgagggcga ggaagccttg cgggcgtgcg acggacggct gcggggtgag attgccgaac
      841 agttccgcgc tgtggtcaat cgtgccgttc gcgttacggt cgagggcgag ccaggcgtcg
      901 tcggagttgg cggcagtcca cgacatgcgg tgacgcacgc cgtcgccgtt gaagtcgaag
      961 tccacgccgc ccgccgcgtc ggtgaagcgg aagccgtcgc ccgccacatc aataatgatg
     1021 gggctgtagt cgcagcacat ctgcggcgtg ctgcccggct ccgtcaaagg gctgtcctcg
     1081 tccgccttaa ggaactgagg cactacttcc gcgttgcttc tggcaaaggc atgcttctta
     1141 ctatcactcg ttaaaagacc gtaactaaca tcgttacggt cttgagaggt gggcacctgt
     1201 tcttttattc ttatccagcc gccgggttta tcaggatcgc ggcgaaagat tcccccgccg
     1261 ctccacttcg tgccatcatc aaaaaccaca tcgccgacac ttatctcaag acgattgatg
     1321 ctggcggggt agtgagtatt attgagaaaa tcccggatgc cctcgtaatc cttcaagacc
     1381 atatccaccg tctcacccgg agggatagga ggctggtcag gatgtggggt cgcaatctca
     1441 cccggcaagg tcgggtattg accataaatt atgcggtcgt gcgcctcagg ctcccctggc
     1501 acacctttat cacgcggaaa actaagctct atttccaaat acgtaatggt tctctccgaa
     1561 gtgtttttta tggtgaaggt tagtcccttc atccaatcat catccgcgcg gaacttttct
     1621 cctacggcta tagctgctcc tttagctttc aatttcccta ttttgacagg ctctttaggc
     1681 cagcttaact tcttaatctc tcgctccttc acttgccctg ccaacccgtg acaggtgaat
     1741 acaaacatac ttacaactaa cagcagagac attacaaaga ctatacctcg acgtgagatt
     1801 gagaacatga ggtgctcctg taagaatatt gttcaaggtc aagctagtca ggatcgaggg
     1861 gttgcgccaa caaagaggcg acgtgagaac cgcgacagcc aacttataaa cccacctcat
     1921 ttacctgtca acatctactc aacggagtga taatccggtg agcaaggtga gtctgcgccc
     1981 gatgtctacg aaagacggtg acgcacgccg tcctcgccgt tgaagtcgaa gtccacgccg
     2041 cccgccgcgt cggtgaagcg gaagccgtcg cccgccacat caataatgat ggggctgtag
     2101 tcgcagcaca tctgcggcgt gctgcccggc tccgtgaagg ggctttcttc atccgtcggg
     2161 catggcacgg caaaccccgt cccgcattcc gtcgtatcgg gagggggcgg cgggcaaggg
     2221 tctaaagaat agtaagtgca tcccactcga ttgccgtcgg catcatcgca aaacttagtt
     2281 tcgtcctcta ttttgccgca ataagacccg ggagagccgc aggtagcgga ttcgacccaa
     2341 gcagcggggc cgcaggtgac acaggtatga ccttcctcca cttccctccc ggagaagcac
     2401 ccgtaaatgc cggggcagca gacacccgct tgaggtgaag caaagtttgg aatcggctca
     2461 actgttctct ccacaaataa ctttgccggt aagaacctta ccggcaagag tgagggggcg
     2521 aattgaaaag aaggggcgag cggaggtttt aatcccccgc cagacgatgc gctctgcgcg
     2581 ggtgctgtct gcctcctgct gccgccgcat gaggaacttg tcgcgttcgg gttgggcacg
     2641 ggcagcccgc cggaagtccc ataacccgtc ccgtcgccgt aatgaagctt gcggaattta
     2701 atgagaaact ttttcggctc cgaccacttg tagtgttttc tggccgactc ccaacccctc
     2761 atctcattct cagggacttt catgacgtaa gtttcgccgg gctggatcgg cacgtcttcc
     2821 gccgtcgggc ggtttctaaa atctccgatg gcaggtcggc cataagaaaa gctcacggca
     2881 acgacactac cggtttcact caacacctcc ggcatacgca gagagaagcg gagataatag
     2941 atcggcttgt cgcccgtatt cttcacctcg acttctacct cgcgcaccca attttctttg
     3001 tcgagattct taatcttcac cttgatcggc aggtgcttcg gaatcttatc ctccaactcc
     3061 cgcgcctccg gcgacgactg cgccgtgctg gagacgttgc cgatgccacc taagacaaga
     3121 aaaaaggcca gcaacgcgat tgctggcaca gttaatttga agcgacgcgg gtgagaaagt
     3181 ctgaagtgag tgttcacgac tgtggctcct gtccggtggg tgagagttgg taaaacgtgt
     3241 gcagcatatt cctgtaggtt agtggagtca atagttatga aacatccttt cccgcactca
     3301 tcgcccgcca cgacatacgg tggcgcacgc cgtcgccgtt gaagtcgaag ttctgcgcgg
     3361 gtgctgtctg ccttcctctc ccgccgcatg aggaactcgt cgcgttcggg ttgggcacgg
     3421 gcagcccgcc ggaagtcata aagcctgttc cgtccccgtg atggagcaag taaaatttga
     3481 ggcggaactt cttcggctct gaccacttac gattgtctct agccgcctcc cagcccgcca
     3541 tatcattctc agggactttc atgacgtaag tttcgccggg ctggatcggt acgtcttccg
     3601 ccgtcgggcg actttcaaag cggcctaatt caggtcggcc atatctaaag gtcacggcaa
     3661 cgacactacc ggtttcagtt atcacttccg gcatctgtag attaaagccc aaataataga
     3721 tcggcttgtc gcccgtattc ttcacctcga cttctacctc gcgcacccag ttctctttgt
     3781 cgagattctt aatcttcact ttaatcggca ggtgcttcgg aatcttatcc tccaactccc
     3841 gcgcctccgg cgacgactgt gccgtacccg gcacgacgcc gaccgcgcac aaggcaagga
     3901 tggcggcgag catcgttagt ttgagattcc gtaagcgaga gaggatagtg tgagtgtgca
     3961 tggcggttgc tcctttacgt gaccgcgttg aggtgagagg cgcgtactga ccagacgggt
     4021 gcagcttagt tgcgccctca gagcgtgtca agagttatgc ggacgcgcgg gcaagccatc
     4081 agtcgcgtag gcgcggagcc gtttgtcgct cctgcaacca atccttgtct cagtcgcttc
     4141 cctcgcctat aatccggctt cctcggatca tagacgactc gaaatgagcg accgcaacta
     4201 aatggcatac ctcaaacagc aacgccccga aaaagttact gtcccttccc tgcgcgccag
     4261 caaagaacgc aaggagcggc tcgtctgcct gacggcttac gactacccga ccgcgcgcat
     4321 cgttgacgaa gcgggcaccg acatcattct cgtcggcgat tcggtcggca acgtcgtcct
     4381 cggctacgac acgaccgtcc ccgtcacgct caaagagatg gtcaggcaca cgcgcgccgt
     4441 gcgtcgcggc gtggagcgcg ccctcttagt cgccgatatg ccctacggct cttatcacac
     4501 gggcgaggac gatgccgtgc gcgccgccct gcggctcatc aaagagggcg gcgcggaggc
     4561 tgtgaagctc gaaggcgggc gggcgcgcga gggcgtcgtg cgtcgcctcg tcgccgaaga
     4621 gattcccgtg atgggacaca tcggtttgac gccgcagtcg gtcaacaagc tcggcggctt
     4681 tcgcctgcaa ggcaagaccg ccgaagccgc acgcgcgctc gtggaggacg cacgcgcgct
     4741 cgaagcggcg ggcgcgttcg cgctcgtgct cgaagtcgtg ccgcgcgaga tcgccagagt
     4801 cgtcacggcg agcgtccgca tcccgaccat cggcatcggc gcaggcgaac actgcgacgc
     4861 gcaggttctc gtcatacacg atctgctcgg cctctccttc agcccgacgc gcccgcgctt
     4921 cgtacgccag tatgcagacc tgcgcgcgca gatgactgag gccatcagcc gcttcgccga
     4981 agacgtgcgc acgggcgcgt acccggcgga gaacgaatcc tacccactgc cacgcgaagc
     5041 cgccgccgaa ctcgaagcga cgcccatcag ccagagcggc gacgcggaga ctgagcgacc
     5101 ttaaaaacag gggtcggagg ctggaggccg ggggtcagtg aaaaaactga ttccggctaa
     5161 gctctggcct ccgacctcta acctctcttc ctataacctc tcttcctatg gaaatcatca
     5221 atcgtcgtca acgcatgaat tcggtgtcgc gcaaggtgcg acgcgagttg gataaaacta
     5281 tcgggctggt gccgacgatg ggcgcgctgc atgaaggcca cctgagcctc gtgcgcgagg
     5341 cgcggcgcat gtgcgatgtc gtggtcgtct cggtcttcgt taaccccaag cagttcgggc
     5401 cacaggaaga cttctcgcgc tacccgcgcg atctgacgaa ggacacgatg gcgctcgccg
     5461 attacaacgt ggactacatc ttcgccccgc cgcccgaaga gatctacccg aaagggtttt
     5521 ccacttacgt cgaagtcgag aggctttccg aaccactcga aggcgcggcg cgtcccggcc
     5581 actttcgcgg cgtcgccacc gtgctcacag tcctcttcaa catcatccgc cccgacttcg
     5641 cctacttcgg ccagaaggac gcgcagcaaa cgctcgtcgt caaacgtctc gtgcgcgacc
     5701 tcgccttcga caccgagatc gtgattctcc cgaccgtgcg cgaagactcc ggcctcgcgc
     5761 tctcctcgcg caacgcctat ctcgaacccg aagacaaacg cgccgccgcc atcatctatc
     5821 gcgcgctcgc acacgccgaa gccgcctacg gcgaaggcga gcgttcgggc aagaaactgg
     5881 cggacatcgt gcgcgcagac atcgaagccg aaccgcgcgc cacgctcgaa tacgtcagcg
     5941 tcaacgacgc cgacacgctg gagcggctcg accgcctcga cgaccgcccc gtcctcatcg
     6001 ccgtcgccgc ccgcctcggc aagacccgcc tcatagacaa catcgtcctc aacaagatga
     6061 gcaagaccgg ccagctcgtc atgccctaaa gccgtaaatc gttaagcgta aatcgtaaat
     6121 agaaggaagc aggttttctc tatttacgat ttacgcttaa cgatttacgg ccttcttgcc
     6181 gtctccgttt cagtcccgcc cccctcttca tataaaatca gtgggatgcc tgaacgaaca
     6241 aaacataaag ctcatattga atttactttg aacggcgaag ccgtcgaagt ggcgttcgcg
     6301 ccgcacaaga cgctgctcga agtcctgcgc gaagatttga atctgatggg cacgaagcac
     6361 ggctgcgaac tcggcgagtg cggcacgtgc gccgtcctcg tcaacggtcg ctcggtgctc
     6421 tcgtgcctcg tgctcggtct ggacgccgaa ggccgcagcg tggaaaccgt cgaaggcatg
     6481 gcaagcccgg cgggacttca cccgttgcag gaaaccttcg cggacacggg cgcggcgcag
     6541 tgcggctact gcacgcccgg cttcctgctc gtcgccaaag aactgctcgc ggtcaatccg
     6601 aaaccgacgc gcgaagagat caaagaagcc ctctccggca atctctgccg ctgcacgggc
     6661 tacatcaaaa tttacgaagc ggtggaactc gcggcggcgc gcatgcgcgg cgaagagttg
     6721 gaactgccgc gcgaagccgt ctacggcctc gaatagttct caccgatgga aattcgacgc
     6781 ctgaaggtag ctcggcctca aagtttttcg gaccgtaata tcctcaaatt atatgccgaa
     6841 caaaatctca cagcacattg acaactttcg ccagaaagta gaagtggcga ataaggaagc
     6901 gacaaaaaaa gaggcattca aagatttact caatcgcctc tacgcggacg acgaagagac
     6961 gcgtaaggtg gttgatgcga ttagcggcgg ggcggagcgc gccgtcgtca acattccgcg
     7021 caaaggtaag cggcatcgag gcagcgccga cacgctctac aatagaacca tcatcgagtt
     7081 tgagaatgat ttgcggaagt ctctcgcgca cggcaaagag caactcgcgg gctatctact
     7141 tggagaaatc aactccgggg agacttacaa tcacacgctg atcgcctccg acttcatcaa
     7201 ctggaaagtt tacgcgcccg atgtgtctca actcgcgatg ttggaaacgg tcaacgaaga
     7261 cgaacttaaa ctcaaagagg ttgaaagcac ctctttcacg ctgacggagc ataacgcgga
     7321 ggagttttac tactggattg atcgctatct gttcagagag aagaagcagc gggcgacgct
     7381 caagcgcatt gcggagtcgt tcggctatca aagccccgtc ttcattcagt gcttccgcga
     7441 cttgacgaaa agttttcgcg ccgtcaaagc gagcggtgag gtgcaggttt cctttgagca
     7501 gtgggaaaag tttctgagta tcgcttacgg ttctttcgtc gccagcgaac agaattttct
     7561 gattcacact tacctgagcg ttttcgccaa actcctcgct tatgcggtcg tctcgaataa
     7621 cgacattgat gaagagaagt tgcgtgaggc aatcgaaggc tcgctcttcg atcaattcaa
     7681 tattaccaac tttgtcgaga acgatttcta tcattgggtc gcaagcgcag agattttccc
     7741 cgcgctgaag gagtcatttc gcggcatcac gcaggagttt gccacctatg attttcagga
     7801 cgtggacgag gacgtgttga agggggtcta tcaggagatg atcgatctcg acacgcgcca
     7861 cgcgctcggc gagtactaca cgcccgactg gctatgcgag cgcgtcgtgg cagagttcaa
     7921 gttccaaccg acggacaaga ttctcgatcc ttcgtgcggc agcggctctt tcctgcgcgc
     7981 cgtgatcggg cgcattcgga aactccaccc gcgcgtgacg gtcgaagatt tgaacgagca
     8041 gatttacggc atagacattc acccgctctc cgtgcagatc gccaaaacga cgctgctcct
     8101 ggcttacggg cgcgcggttg tgaatgctaa gaagcccgtc cacctcaata tcattttggc
     8161 gaacacgctc ctcttgccgg atgaagttag cgatctcttc ggtaagcatt tcacagtgaa
     8221 gattgaccgg aaagactatg agcttgacgt gggcgtgctc gacgatttgc gcgtgttcga
     8281 tgaagcattg aatttaagcg atgatctggc ggagcagacg cggggcaaag gcgcgcaagc
     8341 tttgtccgat ttcaggaacg cgctcgaaca gcaagcgggg gcgggcgaat tgagcgaaga
     8401 ggtagcggag agtttccacc ggatttatca gggcttcaag cgggcgaagg aagatggacg
     8461 cgacagcatc tggaagttta tcgtgcaaaa tctctacaag cctcactttc tcgccgcaaa
     8521 aattgattac atcgtcggca accctccatg gttcacttac agtgacatca ataacgaaga
     8581 ttatcaggtc ttgctgaaca aactcgccgt aaagcatgac gtgaaacctg aacgcagagc
     8641 caacttcccg catctcgaaa tcgcggcgat ctttctcgcc tattgcagcg gcttcttttt
     8701 gcgcgcgggg ggaaagatcg cgttcgtgtt gccgcgtagt ttcttcagcg cggatcagca
     8761 cgacaacacg cgcagcggca aggccaaagg cttcgcgctc accggagcgt gggatttgaa
     8821 agacgtgtcg ccgttattcc gcgtgccgac ttgcgtgctg attggagagc agcgggcgga
     8881 agcggcaact gccgtgaccg tgagcatcga gggcaggacg tttgaaggaa agctaccggc
     8941 gcacaactgc aacctcaaag cagccgcgcc ggaattgact gaggcaagcg ttagatggtt
     9001 ttacgcgaag cagggtcggt caaccgcctt ttcgatgcgt cagcctgatg ccagaaacac
     9061 gaccaaccct tacaagaaaa atttcaagca gggagcgacg attgtgccgc gcgccttcta
     9121 ctttgtggac atcaatcagg agctgccgcc cgacttcgag gagagactca tcaacgtccg
     9181 gacggcggcg tcgattcagc cggaggcgaa aaagccctgg acagagatta cgctcaaggg
     9241 gcgcatcgag agtcgctttc tgtttcgcac cgccctgtcg aaaagtattc tgccgttcgc
     9301 gctctttgcg ccgaacctgg tagcgttgcc tatcactgtc aagcagaaca ccagcgggag
     9361 caaacagctt gaaatgctat cggctgagca attgcgcaag gacggttttc tccacgccgc
     9421 gaaatggttc acggaggtag aaagactatg ggaagaacgt aagaccgaca aatccaggaa
     9481 catgaccaat ctaggtcgcg tcaactacca gggcggctta acggggcaaa atctcgacgc
     9541 gccttatctc gtcctttaca actcttcggc gcaggatgcc aacgcgacgg tagttaagcg
     9601 cacggacttc gatttggagt tcatcgtcga aagcgtggcc tacgtgtttt atacctacgc
     9661 gctcgatgaa gcatactatc tcgcggcaat tttcaattca tctgtgccaa acgagaagat
     9721 gaaagacttc caggccagag gtttattcgg ggcgcgccat gttcataaaa aaatcctcga
     9781 tgtttacttt cccagattcg tcgcgggcga tgacaagcat gcgcgcctcg cccgcctgag
     9841 cgagacggcg cacaaaaaag ccaacgcgtt tttgctcgcc aacccgcccg ccggcgcact
     9901 cacgccccga cgcctcggca ggctacgcgt cgccgtcaag acggaactcg cggcggagtt
     9961 gagcgcgatt gatgaatccg tcaaagagat catcgcctag tcgtctccga atggcggaag
    10021 gcgacacacg gtagagacag gtctgaatta aaaacaatta tgagccagaa caaggacagc
    10081 aaagatttca aagtcgtggg tcggcctttt cgcaaagttg atgcgcgggc gaagtgtgcg
    10141 gggcagacga agtttgccga cgacatcgtc ttgccgcgca tgctcttcgc caaacttttg
    10201 cgcacgcacg aaccgcacgc gcgcatcgtg agcatagaca tctcgaaagc cgtggccttg
    10261 cccggcgtgc acgccgtcat cacgggcaaa gatttgccga ccacctacgg catcctgccc
    10321 gtcagtcagg acgagcacgc cctgtgcgtg gatcgcgtgc gctatatcgg cgacccggtg
    10381 gccgccgtcg ccgccgtgga tgaagacacg gcgtttgcgg cgatgaacct gatcgaagtt
    10441 gagtatgaaa agctgcaaac gatcacgagc atcgaagagg ccataatgat cgacgagccg
    10501 cgtgtgcagg actacggcga gagcggcaac gtccacaaaa aagtccacct cgaattcggc
    10561 gacatggacg aaggcttcgc cgaggccgac cacatccgcg aagacacctt cttctacgaa
    10621 ggcaacacgc acctgccgat ggagcagcac gcggcggtcg cccacttcga cccggacaac
    10681 aagctgacgc tgtggagttc cacgcagacg ccgcactacg tccaccgcgc gctcacgaaa
    10741 gttttaggct tgcccgccgc gcacattcgc gtcatcgcca cgccgaacgg cggcggcttc
    10801 ggcggcaaga gcgacccgtt caaccacgaa gtcgccgtct gcaagctctc gatgatcacg
    10861 ggtcgcccgg tcaaagtaac tctaacgcgc gaagaagttt tctactgtca tcgcggtcgc
    10921 caccccgtcc tgatgaacgt caagacgggc ttcaaacgcg acggctcgat cacggcgatg
    10981 gacttccaga cgtttctcga cggcggcgca tacggctctt acggcgtcgc ctccaccttc
    11041 tacacgggcg cgctgcaaac cgtcacctat gaagtgccgc gctatcgctt tcgcggcctg
    11101 cgcgccttca ccaacaaacc tccctgcggt cccaaacgcg ggcacggcac gccgcaaccg
    11161 cgcttcggcc tcgaagttca actcgacaag atcgcagacg atctcaaact cgacccggcg
    11221 gagatgcgcc tcaagcatct ggtcaaaccc tactcgctca cggccaacta cctgcgcatc
    11281 ggcagcatgg gactcggcaa gtgcatcgag aagatcgtgg aaggctcgga ttggaagaac
    11341 aagtttcgga agctgcctta cggcaagggc gtggggctgg catgcagcag ctacatctgc
    11401 ggcgcggggc tgccgatcta ctggaacaac atgccgcact ccggcgtcca actcggcctc
    11461 gacaggcagg gaggcgtgcg cgtgcagtgc ggctcgaccg acatcgggca aggctctgat
    11521 tccatcctcg cctacatcgt cgccgaagtc ttaggcatag accccttcga catccgcgtc
    11581 ctgaccgccg acacagacct cacgcccgtc gatctcggca gctattcgag ccgcgtcacc
    11641 ctgatgaccg gcaacgccgc gctgcaagcc gccgaacgcg cgcgcgaact actgagccgc
    11701 gccgtcgccg aaaagctctc gctgccggtc gaaaatctct cgttcgcaga ccatcgcgtc
    11761 ttcgacgtgg agaacccgga ggtgggcgtc accttcgccg aggcggtcgt gctcgccgaa
    11821 tcgaaatacg gcacgattgg cacggtcggc tcatatacgc cgccgcgctc gcccggcaag
    11881 ttcaaaggcg cgggcgtcgg accctcgccc gcctacagtt actctgcggc cattgccgaa
    11941 gttgacgttg acgcggagac gggcttcatc acggtcgagc gcatctggat cggccacgac
    12001 atcggtcagt cgatcaaccc gatgctcgtc atgggacagg tcgaaggcgg cgtctacatg
    12061 ggcttaggcg aagcgttgat ggaggagatg atctatcgcg ccaaccgcaa cgtcgtgcac
    12121 aaattccctt cgctcctcga atacaagagt ccgacgacgc acgagatgtg cgatgtcaaa
    12181 acttatctca tcgaagacgc cgacccgaat ggtcccttcg gcgcgaagga agtgggacag
    12241 gggccgctgc tgcccgtcat gccggcggtg gctaacgccg tctacgatgc cgtgggcgtg
    12301 cgcgtggacg aagtgccgat cacgcccgaa aaagttctgg ccgcgttgcg cgccaaagcg
    12361 cagggcaaac ccgcgcgctt cggacccaaa ggcgtgcccg tcgtcgaatg gccggaaccg
    12421 ctcaaagtcg ccacccctgc ggagggcggc gacgggcgcg agatgccgcg cgtggcgatt
    12481 cattcgtaag cacgcgtctc cgttttatct cttttgctac ggcatgttct ttatcgaagg
    12541 tcgctcgcgc gtttactatc accactgaat ccgacaagga gtttgaagat gatcgccgca
    12601 cctcgtaagt ttttttcgct ccgtgcagca tgcgcaggga tttgcgcgct ggcgcttgga
    12661 ttgacacttt ccgcttggcc gcaaacgtcg tcggcgcagg acaagatcac aacgcaactc
    12721 ggcgactcgg ctgaaggagc agccatcttc aagacgccga agggctacat gcagattaag
    12781 ctcgaagggt tcagaggcgt cttgatcctc gacccgaaga agcccgcagg gatgtttatc
    12841 tcttatcctg acgacaagga gacgaccgaa tccttacgcc gacgcgttcg cacgagggtc
    12901 acggaaatgt tcattcacgg cgacaagacg ccggaactgg actggagcgc caaaccgctc
    12961 ccgccgcaca agggcgacgg cgaagggaaa gcgtcgctgg agacggccac gtcgggcgag
    13021 acggagattc aacttgcgac ttacgagcgc acaacgggcg ttcgcccttt cgtctatggt
    13081 tacttcgcca tgcgcaacaa gtcgggtaaa ggcgaaaaag tgcacttcat tgacgagcag
    13141 ggtctagggg tcaagaagtt cgacgaactc tggcaatcaa tgcccggctc gaaatagaga
    13201 aggcggcaac agcgggagca tcgtgcggcg cgtacgtcgc cggtgacgct cctctgtcgc
    13261 tgttatcatt cgcggttgtc atttgaaaaa catgagggtt attgaatcgt tatgatgaga
    13321 cttccgaagt ttgagtatcg cgtgccgaga gaagtgaggg acgcggtgaa gatgatggcg
    13381 gacgcgggcg cgcaggggca gttcgtcgcg ggcgggacgg acttgtatcc gaatatgaag
    13441 cggctgcaac agacgccgaa gatcgtgatc tccgtggggc gcatcgcaga gttgaataag
    13501 atcgagggcg acggcaagag cggcgtgcgc gtgggcgcgg gcgtgtcgct cacggatttg
    13561 tgcgagcacc ccgtgatcaa tcgcgattac ccgatggtgg cggcggcggc gcgcacgatc
    13621 tcgacgcccg tgctgcgcaa catgggaacg attggcggca atcttctgct cgacacgcgc
    13681 tgcaattact acaaccagaa ttacgagtgg cgcaagggcg tcaatttctg catgaagaag
    13741 gacggcgacg tgtgctgggt tgcgcccggc tcttcgaagt gctgggcggt gcagtcagct
    13801 gatctcgtgc cgctgatggt cgcgctcggc gcgcgcgtgc gtttcgtctc gacgctcggc
    13861 gagcgcgagg tgaccgccgc cgggttgtat aacgacgacg gcatagacta cctgcataag
    13921 cgtcccgatg aactgctggc cgacattcaa ctgccgcctc tgaacggctg gcgcgcagtc
    13981 tataaaaaac tgagacggcg cggcgcgttc gatttcccgg tgctcggcgt cgccgcgcga
    14041 ctcgacatgg aagcggacgg gacggtgaag gatgcgaagt tgattctggg cggcgtcgcg
    14101 cccgcgcccc tctccgtgac ggaagcgcaa aatgctttga tcggcgcgcc gctcgacgcc
    14161 gaacgcatac gcgcggcagc cgaagcctgc tacgtcaaag cccgaccgct cgacaacacg
    14221 gatttcatca tgaactggcg caagcagatg acgcgcatct acgtcgtgcg tgcgctcgaa
    14281 tctttgcaag gcgagtagcg ggtcattttt cggaaacagt tgccgcgccc ggcgcgttta
    14341 aggtggagac atagcagtcc gcccaatctg aaatttccga aattctggag acgttaaatg
    14401 aaaaagttga tcttaattat cgcggctctc gtgtcgctgt cctccgccgc cttcgcgcaa
    14461 cagggtgcgg aggccgagcg tgaggcggtg aagcaggccg tgctggatta cgtcgaaggc
    14521 atctacacgg ttgacgcttc gcgcatcgaa cgcagcgtcc accccgaact ggcgaagcgc
    14581 ggcttcttcg tgaagcgggg cgaaaccgcc tacagttccg acgtgatgac ctttcagcaa
    14641 ctcgtcgaac tcgccaagac ctacaacaag aacgggcgcg tgccgaagga ggccccgaag
    14701 gaagtggtca tctacgacgt gtccgaccag accgcgagcg cgaaactcac agccgtctgg
    14761 ggcatagact atatccatct cgccaagtac gacggcaagt ggaagttcgt caacgtgctc
    14821 tggcagactc cgcccaagaa ataaggatga ggaatgaggg atgagggatg aaataagaaa
    14881 ggcattcaag aggttgagtg ctttcgttca tccctcatcc ctcatccctc atccctcatc
    14941 cctcatccct catcccttgc ctgtcgccgc cctcctcctc gccgccggac gctcgcggcg
    15001 catgggcgcg ttcaagcctc tcctgccctt cggcgcgcag accgtcatcg aaacctgcat
    15061 caaccaactg ctcgcggctg gctgcgaaga gatcgtcgtc gtcgtcggcc atcgcgcgga
    15121 agaagtgcgc gcgcgcgtgt cgcaccttcc cgtccgtttc gcctgcaacg atgaagcggg
    15181 gagcgagatg ggcgtctcga ttgcgcgggg cgtggaagaa ttgacggcgg gcgaccgcgc
    15241 cgcagcagcg acgacgacga cgccgggcgc agtcttgatc gcgctcgcag accacccggc
    15301 ggtgacggct caaacaatcg aagaggtcat cgcagcgcac cgtcgcacgg gcgcgcggct
    15361 cgtcgtgccg gaatggcagg ggcgcggcgg gcatccggtg ttagtcagca tggggcttcg
    15421 cgcgtcgctc ctgaatcttg actccgtggg cgggctgcgc tcgctctttg cggcgcacgc
    15481 gggcgaggtt ttgcgcgtgc ccgtcgcgtc cggctacatt gcgcgcgata tggatacgtg
    15541 ggaagattac cgcacgctgc atgaggaagt tttcggcgtc ccgccgcccg tcacactttt
    15601 gtccgggcag caaacttgaa gaggcttctg tcccgcttca atccaactgt tctttctgac
    15661 cgtacatcta actactcggt acgggagctt ttcacgcaca ggccgggtgc gacgcgggcg
    15721 acggacaagt cagccccgaa ctgcaactcg atagcgtgag acgcccacta gaaggagaaa
    15781 agaatgttta tcaagtcccc ccgtttcatc ctcagtctgg tgctctcctc aatgctcgtc
    15841 ttcgcgcagg cgctcgcttc cacgcccgcg ccagtcaaga cacaggaggg cttgttggac
    15901 ttttcgctgc gctcggttga cggcggcagc atcacctccg agcagcttcg cggcgacgta
    15961 gtgatcctcg ctttcggcgc atcgtggctg ccgctctcgc gcacgcaggt tgaaggcgtg
    16021 cagcggctcg cggacgaata ccgcgaacgc gatctcaggg tctactgggt gagcacggat
    16081 tccgaatcgc agaaatcgaa gaacttcgcg acggacgatc aactgcgttc gttcgcgcgc
    16141 aagaacgggc tgaagatcgg cgtcctgcgc gaccccgacg gcgcgctctt caaacgcctc
    16201 ggcgttgatc gcaaccaact gcccgcgctc gtcatcttcg acaaagaagg acagatttcc
    16261 ggcgcgccca tcggcggact cgaccccggc ggcaacctcg tccaacgcct ctccgcgcgc
    16321 atcaacgcgc tgctctgaac agcaagtagg aagcgggaag gatgaacgac gaagggaagc
    16381 actcaggaga ctgaatgctt cccttcgtcg ttcatccttc ccgcttccta cttaacttcg
    16441 gctctcagcc gccaacttgt agacgagccg gatcggcaac ccgacgatgt tccagtagtc
    16501 gccctcgatc tcttcgatga agagcgcggc gtgcccctgt atggcatacg cgcccgcctt
    16561 gtcggcaggc tcgcccgtcg atacgtacca gtcaatctcc gcatccgaca tcgaagcgaa
    16621 acgcacgcgc gtcagttcgt gcgccaccag cacctcgttc gtctcggcgc gcacgagcgc
    16681 aactccgctc agcacgtcat gccacctgcc gtcgagcaac ttgagcatcc gccgcgcgtc
    16741 cgcctcgtcc ttcggcttgc cgagcacctg ccccgccacc acgacgaccg tgtccgcgcc
    16801 gagcaccagc ccttttgttc tccccgccgc cactgcttcc gccttctcgc acgccagccg
    16861 ctccacgtat tcgaccgcgc cttcgccctc ccgcgcagac tcgtcgatgt ccgccgcctc
    16921 cacttcaaaa ggccaattcg tccggcgcaa aatctccgcg cgtcgcgggc tgcccgaagc
    16981 cagcaccagt tgttcgagcg cgaacccgcc cgcgccgccc gccgaagcct ctacttgtcg
    17041 ctctccacga ttttccatta cactccctaa cgattgagga tttcagatta cggaatgcgg
    17101 attctaaatt gattaagccg ggattgccaa tccgcgctcc gcacctactg atcggtcttt
    17161 cgcaatccgg agtccgcaat tgaaaagatg gacgatctaa ttcgatcact gcccaacctg
    17221 ctgcgtgcgg cgggcgacgc ggaagaggtg gccgaagcgg cggcgatggt ggcctggcgt
    17281 cgcgtggcgg gcgaagcctt gcgcgggcag gccgtgccgt ttcgacttca tcgcaaactg
    17341 ctggtcgtcg ccgtgcccga taccgcctgg cagaaacaac tcgaatccat cagcggccag
    17401 ctcatctttc gtctcaactc gatgctcggt caggccgtcg tcacctacat agagtttcgc
    17461 gtcgatccga agaccgtccg cgcagaacgc gcacggctcg gccagaccga atttgaccgc
    17521 gccgcacatg aacgacgctc gctgcaaaac gcgcgtcaac tcacggacgc cgccgacgcc
    17581 attcaggacg aagacctgcg ccgccgcttc ctgctcgccg ccggaagcta catcggcgcg
    17641 caacagaaga acaagtgagc agtgagcggc aggcagtaag cagttaaaaa cagtcttcct
    17701 actgctcgcc gcctgctgct caacgcttac cgcttactct taacttatat gcccatcaca
    17761 gaatccgaca tcgaaaagat cgccgcgctt gcgcaactgg agatcacgga cgaagagcgt
    17821 cgcgcgttga cgccgcagat cgcttcgatt gtgacctacg tggagcagtt gaacgaactg
    17881 gacacgagtg cggcggaacc cgctctcggc gggctgacgc cggaaggcgc gcgcaccgac
    17941 accgcgcgcg aagatgcacc gctcccctcc ctcggccagt cgctcgcgct cgatcaagcg
    18001 cccgacgcag ccgccggaca cttccgcgtc cctaaagttt tgtaaatagc cgtcagccgt
    18061 gcgccgttag cttcaatgct tcggcccacc gccgacagct aatagctgac aaccatttta
    18121 tgagcatcga aaacatcagg agacgcctcg cgagaggcga acaggacgca cgcgccgcct
    18181 cggagactgc gctcaacgct gcggaagagt tgaacgagac gctgcacgcg tttttgcaga
    18241 tcgaccgcgc gggcgcgctc gcacgcgtgg acgcgctcga cgcgcgacgc gacgagtcgt
    18301 tgaacggcgc gctcggcgca gtgcccgttg ccatcaaaga caacatctgc gtgcgcggcc
    18361 tgcaaacttc gtgcggctct gccatcctcg gcgattatca accgccctac aacgccaccg
    18421 tcatcgaacg cctgctggac gccggcgcaa tcatcgtcgg caagaccaac tgcgacgagt
    18481 ttgcgatggg atcgtcaaat gagaattcgg ccttcggtgc ggtgcgcaac ccttgggaca
    18541 caaagcgcgt gcccggcgga tcgtcgggcg gttcggcggc ggcggtcgcg gcgcgcatcg
    18601 tgcccgtcgc tctggggtcg gacacgggcg gttcggtgcg ccaaccggcg tcgctctgcg
    18661 gcatcgtcgg cgtcaaaccc acttacgggc gcgtctcgcg ttacggcctt gtcgccttcg
    18721 gctcgtcgct cgatcaagtc ggcgtcttcg ccacgaacac gcacgacgcg gcattgaccc
    18781 tctccgtcat cgccggacgc gatgcgcgcg acgcgacgac cgccgacatg gtcgtgcccg
    18841 attacgtctc
//
LOCUS       DASWNS010000443         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_250796, whole genome shotgun sequence.
ACCESSION   DASWNS010000443 DASWNS010000000
VERSION     DASWNS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250796"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="VGB76_15280"
     CDS             complement(<1..1662)
                     /locus_tag="VGB76_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0100311.1"
                     /translation="MRSLSHAKQRARALSALIGAGSEGLFEGIKDYLFKEHRIRVRAV
                     EAHEINDNEAELSVFERELCYVKRLNADPVQRLLVVAHELGHLDLHKRLTDQLSGTTQ
                     LLPSIYLGRGAPALARYNSRAREEIEADAFAKEFVCSSGEIFEQWLRDQSATSKSLAA
                     ERGLPEDFVRVQLVEGLSDMALEHSPEVKDEPRRALLPDETQVAAATHVGTPALVNAG
                     PGTGKTSTLVMRIEYLRREKGAQPRQMLVLTFSNEAADKLRERVAEKFDAETASEMEI
                     TTFHSFGYSFLLTHGHRIGLDPEITVLDEARQEELINGLLGRVDCDAIIDLKQPEETA
                     RQALRQICRLKERRVSPDKLEEAIDQWGVEEPEQKPVQERSRALLSIFREYERVKTGL
                     NAVDFADLIVMPLDLLERDKELAASLRNHHKWVMIDEYQDVSRAVAMLLGELCSEDNP
                     PWVVGDLRQAIYLFCGASRQNVSHFPEDFRGAKIFELETNYRSCDEVINVANQLAELM
                     ECPGKADDSYSERWRRGTDTKSLEADGGAVVVAQADSDDAEYEGIA"
     gene            complement(1668..2672)
                     /locus_tag="VGB76_15285"
     CDS             complement(1668..2672)
                     /locus_tag="VGB76_15285"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEY0100312.1"
                     /translation="METKKKFDEEYRQAKQAGERANAVEPRALSARYDPDSRQVIIRL
                     RGGEDLAFSPELFPNLRGAAPGDLKEVEVTPSGEGLHWESLDEDLSVPALLQEVYGPR
                     AAGDGSPRFGVELPESLCAQLEMAWVTGRDDMAVDRLAAEHPEHAEALYDFFGLLVES
                     ELAAPPQSDAGKSAYRTLSWLREEGFALANRIAREESGQTPEASPDAPAGVAVEPRPT
                     AQVQAVPLAYLGLLQERTGRDVDEITGSMDVEASVIKFVQRQPADKFKRVRGEIVRRG
                     AEIWGIGEEEGAEALALQLPMAALRKSSAESLTYEQVINRSGMSSEKKRLWLELASED
                     "
     gene            complement(2650..2934)
                     /locus_tag="VGB76_15290"
     CDS             complement(2650..2934)
                     /locus_tag="VGB76_15290"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEY0100313.1"
                     /translation="MARFIYTDMPTVLRQNGFKVRIYTNDHLPSHVHVFKAEGELIFA
                     LGSESEAPRMVKELSPMNPSDARQARVIVKEQQQYLLREWRKIHGNQKEV"
BASE COUNT          531 a          946 c          848 g          684 t
ORIGIN      
        1 agcgatgccc tcgtattcgg cgtcgtcaga atcagcctgc gctaccacca cggcaccccc
       61 atctgcttcg agcgacttgg tgtcagtccc acgcctccaa cgctcggagt atgagtcatc
      121 agccttccca gggcattcca tcagttcggc taactggttg gcgacgttaa tcacctcgtc
      181 acaggagcgg taattcgtct ctagctcgaa tatcttcgcg ccgcgaaaat cttcagggaa
      241 atggctgacg ttctggcgcg acgcaccgca aaagagataa atcgcctgcc gcaggtcacc
      301 gaccacccac ggcgggttgt cctcgctgca aagttcaccc agcaacatgg cgactgctcg
      361 gctgacgtcc tgatactcgt caatcatcac ccacttgtgg tggttgcgta ggctcgcggc
      421 tagctccttg tcccgttcga gaagatctag tggcatgaca atcagatcgg cgaagtccac
      481 cgcgttgagg ccggttttaa cccgctcgta ctctcggaat attgagagca aggcgcgcga
      541 tctctcttgg acgggctttt gttccggctc ttcgacgccc cactggtcga tagcctcctc
      601 caacttgtcg gggctgacgc gccgctcttt gaggcggcag atctgccgca acgcctggcg
      661 cgccgtctct tcgggctgct tcaagtcgat gatcgcgtcg caatcgaccc tccccagcag
      721 cccgtttatg agttcctctt ggcgtgcctc gtccagaacg gttatctccg gatcgagtcc
      781 gattcggtga ccgtgtgtga gcaggaacga gtagccgaag ctgtggaagg tcgtgatctc
      841 catctctgac gccgtctcgg cgtcgaactt ctccgccacc cgctcgcgca gtttgtcagc
      901 agcctcgttg ctgaaggtga gcactagcat ttgcctcggc tgggcgccct tctccctccg
      961 caggtactcg atccgcatta cgagcgtcga tgttttaccc gtccccggtc ccgcgttgac
     1021 gagtgctggg gtgcccacgt gggtcgccgc cgcgacctgt gtctcgtcgg gcaacaaggc
     1081 ccgacgcggc tcgtccttaa cttcgggcga gtgttccagt gccatatcag acagaccttc
     1141 gacgagttgc accctaacga aatcttcggg taacccgcgt tcggcagcga gtgactttga
     1201 cgtcgccgat tggtcgcgta gccactgttc aaagatttcg ccgctcgaac aaacaaactc
     1261 tttagcgaag gcgtcggctt cgatctcttc gcgcgctcgg ctgttgtaac gcgcgagggc
     1321 gggggcgccc ctgccgaggt agatggaagg gagcagttgg gtggttcctg agagctgatc
     1381 ggtcaggcgc ttgtgaaggt caaggtgccc cagttcgtgc gcgacgacta gcagtctttg
     1441 gaccgggtct gcgttgaggc gtttgacgta acaaagttcc ctttcgaaga cgctcagttc
     1501 tgcctcgttg tcgttaatct cgtgcgcctc cacggcgcgc acccggatgc ggtgctcttt
     1561 gaagaggtag tccttgatgc cttcgaacag accttcgctt cccgccccaa ttagggcact
     1621 gagagcgcgg gctcgttgtt tcgcgtgact cagcgacctc atgcacctca atcctcacta
     1681 gccagttcca gccagagcct tttcttctcg gagctcatcc ctgaccggtt aatgacctgc
     1741 tcgtatgtca gcgactctgc cgacgacttg cgcagcgccg ccatgggcag ctgtagggca
     1801 agcgcctcag ccccttcctc ctcacctatg ccccagatct cggcaccccg gcggacgatc
     1861 tcgcctcgca cgcgcttaaa tttgtctgcg ggctgacgtt gaacgaactt gatgacggaa
     1921 gcctcgacat ccatgcttcc tgtgatttca tcaacgtcgc ggcccgtacg ttcctgtaat
     1981 agacctaggt aagcgagagg caccgcttga acctgcgccg tcggtctcgg ttcgaccgcg
     2041 acgccagcgg gcgcgtctgg cgaggcttcc ggtgtctgtc ccgactcctc ccttgcgatc
     2101 ctgttagcca aggcgaaccc ttcctcccgt agccaagaca gtgtgcggta agccgatttt
     2161 ccggcgtccg actgcggtgg cgcagccagt tcgctctcga ccagcagccc aaaaaagtca
     2221 taaagagctt cggcgtgctc ggggtgttca gcagccagcc gatcaaccgc catgtcatca
     2281 cgcccggtaa cccatgccat ttctaactgc gcgcacaagc tttcgggcag ctctacgccg
     2341 aaacgagggg atccgtctcc cgccgccctc ggtccgtaga cttcttgcag caaagccggg
     2401 acgctcaaat cctcgtccag actctcccag tggagacctt cgccggaggg tgtcacttcg
     2461 acctccttca ggtccccggg ggcagcgcca cgaaggtttg ggaatagctc cggggagaag
     2521 gcgagatctt cccctcccct caggcggata atcacttggc gtgaatcagg gtcgtagcgg
     2581 gcggagaggg cgcggggctc gaccgcgttg gcgcgctcgc ctgcctgttt cgcctgcctg
     2641 tattcttcgt caaacttctt tttggtttcc atgaatcttt ctccattctc ggagcaggta
     2701 ctgctgttgc tctttcacga tgacgcgtgc ttggcgcgca tccgatgggt tcatgggaga
     2761 caactcttta accatgcgcg gggcttcgga ttcgcttcca agggcgaata tgagttcgcc
     2821 ctccgcctta aacacgtgca cgtgggaagg taggtggtcg ttagtataaa tgcgcacctt
     2881 aaaaccattt tgtcttagta ccgtcggcat gtcggtgtag atgaaccgcg ccatctccga
     2941 tcaactactg caggaagcgc cgtagccact cggcgtcaat ttcgtgttgg cgctgtgcaa
     3001 gcagcgccg
//
LOCUS       DASWNS010000444         6480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_485560, whole genome shotgun sequence.
ACCESSION   DASWNS010000444 DASWNS010000000
VERSION     DASWNS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485560"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /gene="nrdR"
                     /locus_tag="VGB76_15295"
     CDS             <1..504
                     /gene="nrdR"
                     /locus_tag="VGB76_15295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY0100314.1"
                     /translation="GVSMKCPFCGYLEDKVVDSRESREGDAIRRRRECLKCERRFTSY
                     ERIDEIPYMVIKKDGRREPFDRTKIMAGLLRACEKRPVPSSKLDGIVNQIEKYVQESP
                     ERERPTSKIGEMIMRRLKELDKVAYVRFASVYLEFEDVTEFMHELKTLVRSRQSGSAG
                     SAATKRQ"
     gene            597..1067
                     /locus_tag="VGB76_15300"
     CDS             597..1067
                     /locus_tag="VGB76_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0100315.1"
                     /translation="MRNVGRVVGLERIEIERLRNRVGRLLAALQEVADDGSLAVPGAW
                     SPPVDLCESEDAVCVSVELPGVPPDSIEVSLTSSYLRISGKKKRRVLRGSISHLCSER
                     SFGNFNRTIPLRWPVRVRGATAELKNGLLRVRLPKLADRRGAEFKVQIKERDEG"
     gene            1162..3612
                     /gene="lon"
                     /locus_tag="VGB76_15305"
     CDS             1162..3612
                     /gene="lon"
                     /locus_tag="VGB76_15305"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0100316.1"
                     /translation="MPSITDIAEGLEAATEGGEQQLQIPETLPVLPLRDIVIYPFMIV
                     PLFVSREKSIRAVDEALGENRMILLASQKDLDKEEPTGDDLYRVGTVAVIMRMLKLPD
                     GRTRILVQGLTRAHLESIEEGGEYLRARVNVMQEPPTPERSLEVEALVRNVRASMEKA
                     ANLGKNISPEVMAIVANLDEAGRLADLAASNLELKVEDAQSVLDIADTTARLRRVNEL
                     LNKEIEVLTVQQEINTQARADIDRSQREFYLRQQLKAIQGELGEGNELAEEIAQFREK
                     IEAAHMPKAAEEESLRQLKKLERMHPDAAETATLRNWMEIMTDLPWSKASTDNLDLKK
                     AEEVLNEDHYGLEKIKERIIEALAVRKLKEKPKGSILCLVGPPGVGKTSLGRSIARAL
                     DRKFVRLSLGGLHDEAEIRGHRRTYVGAMPGRIVQALQQAATNNPLIMLDEIDKVGAD
                     FRGDPSSALLEVLDPEQNNSFRDNYLGVTFDLSNVMFMTTANVLDTIQPALRDRMEVI
                     RLAGYTEEEKLEIARRHLIPKQLDENGIASRHLRISKGALAALILQYTQEAGLRQLER
                     EIGAICRKVARRIAEGSNDTVHVTPKNLHEFLGVPKVMPDEVLKKDQIGVATGLAWTA
                     VGGEVLFIEALRMKGKGNLVLTGQLGDVMKESAQAAYSYAKSRARELGIPEDDFNIYD
                     VHVHIPEGAIPKDGPSAGITLATAIVSVLAQRPIRKDVAMTGEITLRGNVLPVGGVKE
                     KVLAARRARVTKILLPHLNQRDMDEVPKELFGEIQFIFVENVQQVFREALKDKVTPPA
                     SGSARPKPLTLPVPASSH"
     gene            4046..5740
                     /locus_tag="VGB76_15310"
     CDS             4046..5740
                     /locus_tag="VGB76_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0100317.1"
                     /translation="MLVSPLATAYAQGLPSSLRTTVNDVERDNPRVLQIMEKAEQHYR
                     LGELNLKDKNMDAARTEFDKAVDSVLESGMDVRSNPKLQTFYLQLVERVYRMEVAMPA
                     PTQNSGQPAQFVAASTNATNDVAAVAAQQPGFLREQKFEPSPLDELSRLELTTEEKQV
                     TNEDVVKLEEAKNAVDFAFNSNNLIQGFINYYQGRGRATMETGLRRSGRYISMARRIF
                     REEGVPEDIVWLGQVESAWRPTAYSWAAASGLWQFIPSTGAEYGLRQNAWIDERNSFE
                     KATRASARYLKHLNRYYNGNWELALGAYNTGPGNIDRGIRRGGAASFWAAYPYIAQET
                     RNYVPNILATILIAKNPAKYGFHNIRPDAPLAYDVVNVPSATSLQLIASATDTSVDYI
                     RSLNPELRRDTTPRGESYNLRVPPGRGKSFVALLKRIPGDRRESAKVISVAPGEDLQS
                     VAARTGTSVAQLQMWNSGVDLKGGGKVVVPAGSVRSVAMVRPRGNVGSSLISVRARAG
                     ETLAQLAARQGVGADEVARLNAISPDAPLSANQEIKVPSKSPASTSGGTAPVRRRR"
     gene            5937..>6480
                     /locus_tag="VGB76_15315"
     CDS             5937..>6480
                     /locus_tag="VGB76_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEY0100318.1"
                     /translation="MLFRTQSAAVYGIDADLVDIEVDLTPARNEADTPASVTIVGLPD
                     AAVRESRERIRAAINNSAFFFPFHKTTINLAPADVRKEGASFDLPIALGILGANGDLG
                     ERDHLTDALSVGELSLDGRVRAIKGALSIAIRARDAGIRFLLLPEENATEAAVVAGVD
                     VYPVTDLRAAVELVAALRKSD"
BASE COUNT         1381 a         1966 c         1945 g         1188 t
ORIGIN      
        1 ggagtctcta tgaaatgtcc tttctgcgga tacctcgaag ataaagtggt tgactcgcgt
       61 gaatcgcgcg agggcgacgc gattcgtcgt cgccgcgagt gcctcaagtg cgagcgacgc
      121 ttcacgtctt acgagcgcat cgacgaaatc ccttacatgg ttatcaaaaa ggacgggcgg
      181 cgcgagccgt tcgaccgcac gaagatcatg gccgggctgt tgcgcgcctg cgaaaaacga
      241 cccgtgcctt cgtcgaagct cgacggcatc gtcaaccaga tcgaaaagta cgtgcaggag
      301 tcgccggagc gcgaacgccc gacgagcaag ataggcgaga tgatcatgcg ccgcctcaaa
      361 gaactcgaca aggtggccta cgtgcgcttc gcctccgtct atctcgaatt tgaagacgta
      421 accgaattta tgcacgaact gaaaacgctc gtgcgctcgc gccagagcgg cagcgcaggc
      481 agcgccgcga ctaaaaggca gtgatgagtg acaggtgacg agtgacaagt tgaaaccaaa
      541 cgcttgtctg gattctcgct cgtcacctgt tagtcgtcac tcatcactta aaaaagatga
      601 gaaacgtagg gcgagttgtc gggttggagc ggattgagat tgagcggctg cgcaatcgcg
      661 tggggcgtct gctggcggct ttgcaggagg tggcggacga cggttcgctg gccgtgccgg
      721 gcgcatggtc gccgccggtt gatctgtgtg agagtgagga tgcggtgtgc gtctcggtgg
      781 aactgccggg cgtgccgccc gactctatcg aggtcagtct gacgagcagt tacctgcgga
      841 tcagcggcaa aaagaagcga cgcgtacttc gcggctcgat ctcgcatcta tgttcagagc
      901 gaagtttcgg aaacttcaat cgcaccattc cgctgcgctg gcctgtgcgt gtgcgcggcg
      961 caacagcgga actgaaaaac ggattgctac gcgttcgtct gcccaaactc gcagaccggc
     1021 gcggcgcgga atttaaagta cagattaagg aaagggatga aggatgaggg cggagggatg
     1081 aataaaacag aagctagtta cccggcttga atgttttctg attcatccct ccgccctcat
     1141 ccttcatcct taaaaatata tatgccatct atcacagaca tcgcagaagg attggaagcg
     1201 gcgaccgagg gcggcgagca gcagttgcag attcctgaga cgctccccgt gttgccgttg
     1261 cgcgacattg ttatctaccc gttcatgatc gtgccgctgt ttgtctcgcg cgaaaaatcg
     1321 atccgcgccg tggacgaagc gctcggcgag aaccgcatga tcctgctcgc ctcgcagaaa
     1381 gacctcgaca aggaagagcc gacgggcgac gacctctacc gcgtcggcac ggtcgccgtc
     1441 atcatgcgca tgttgaaact gcccgacggg cgcacgcgca ttctcgtgca gggcttgacg
     1501 cgcgcgcatc tcgaatcaat cgaagaaggc ggcgagtatc tgcgcgcacg cgtgaatgtg
     1561 atgcaggagc cgcccacgcc ggagcgttcg ctggaagtcg aagcgttagt cagaaacgtg
     1621 cgcgcctcga tggagaaggc cgcgaacctc ggcaagaaca tctcgcccga agtgatggcg
     1681 attgtcgcca acctggatga agcgggacgc ctcgcagacc tcgccgcgtc gaatctcgaa
     1741 ctcaaggtcg aagacgcgca gagcgtcctc gacatcgcgg acacgaccgc tcgcctgcgt
     1801 cgcgtcaacg aactcctgaa caaagagatc gaagtcctga ccgtccagca ggagatcaac
     1861 acgcaggcgc gcgccgacat tgatcgctct cagcgcgagt tctacctgcg ccagcaactc
     1921 aaggcgattc agggcgaact cggcgaaggc aacgaactcg ccgaagagat cgcgcagttt
     1981 cgtgaaaaga tcgaagcggc gcacatgccg aaggctgccg aggaagagtc tctgcgccaa
     2041 ctcaagaaac tcgaacgcat gcacccggac gccgccgaga cggcgacttt gcgtaactgg
     2101 atggagatca tgaccgacct cccctggtcg aaggcttcga cggacaatct cgacttgaag
     2161 aaggccgagg aggtgttgaa cgaagaccac tacggcctcg aaaagatcaa agagcgcatt
     2221 atcgaagctc tggccgtgcg caagttgaag gagaagccga agggttcgat cctgtgcctc
     2281 gtaggcccgc cgggcgtggg caaaaccagc ttaggacgtt ccattgcgcg tgcgcttgat
     2341 cgcaagttcg tgcgtctcag tttaggcggt ctgcacgacg aagcggagat acgcggccac
     2401 cgtcgcacct acgtcggcgc gatgcccggt cgcatcgtgc aggctctgca acaggcggcg
     2461 acaaacaacc cgctcatcat gctcgacgag atcgacaaag tcggcgcgga ttttcgcggc
     2521 gacccgtcat ccgcgctcct cgaagtgctc gaccccgaac agaacaacag ttttcgcgac
     2581 aactatctcg gcgtcacctt cgacctgtcc aacgtgatgt tcatgacgac cgccaacgtg
     2641 ctcgacacga tccagccagc cctgcgcgac cgcatggaag tgatccggct cgcgggctac
     2701 acggaggagg agaagttgga gatcgctcgc cgccatctca tcccgaagca gttggacgaa
     2761 aacggcatcg cctcgcgcca cctgcgcatc tcgaagggcg cgctcgccgc gctcatcctg
     2821 caatacacgc aggaggcggg cttgcgccaa ctcgaacgag agatcggcgc gatctgtcgc
     2881 aaggtggcac gccgcatcgc ggaaggctcg aacgacaccg tgcacgtcac gccgaaaaac
     2941 ctgcacgagt tcttaggcgt gccgaaagtg atgcccgacg aggtgctcaa aaaagatcag
     3001 atcggcgtgg cgaccgggtt ggcctggacg gctgtgggcg gcgaagtgct cttcatagag
     3061 gctctgagga tgaaagggaa gggcaatctc gtcctgaccg gccaactcgg cgacgtgatg
     3121 aaggagtcgg cgcaggccgc ctactcctac gccaagtcgc gcgcacgcga actcggcatt
     3181 cccgaagacg acttcaacat ctacgacgtg cacgtacaca tccccgaagg cgcaatcccg
     3241 aaggacggcc cctccgcagg catcactctg gcgacggcca ttgtctccgt gctcgcgcag
     3301 cgtcccatac gcaaagacgt ggcgatgacg ggcgagatca ccctgcgcgg aaacgtcctg
     3361 cccgtgggcg gcgtgaagga gaaggtgctg gcagcgcgtc gcgcgcgggt gacgaagatt
     3421 ctcctgccgc atttaaacca gcgcgatatg gacgaagtgc cgaaggagtt gttcggcgaa
     3481 atccagttca tcttcgtgga gaacgtccag caggtctttc gcgaggcttt gaaggacaaa
     3541 gtgacgccgc ccgcctccgg ttcggcgcgc ccgaaaccgc tgacgctgcc cgtcccggcc
     3601 tcttcgcatt gaaattatgc gtaaattcgg cacgcgggaa acgctgtgtg gagggtttca
     3661 aaacgctcaa attggttgat tagaaaacgc aacccgagca caacaaaaag cgtcttaata
     3721 gagggaaaag cgaagcggtt ttgatggaaa tctcgaccgc ttcgcctttt ttatcaacta
     3781 gataaggttg actccctaaa aacggtgtga taagttccaa gactgccgcg cacggctttc
     3841 ttacgacagt taagccacag tgcgaaacag ccttttccat tgaattcata accctcgcgt
     3901 ttttttgtag tggatgccgc tcgtgtggtc tgccaagtct cctgaccgcc tcgtggattc
     3961 gctttcagcc tgaagtttaa ggagaatgac gattttgatg agaacgagct tcccccgatt
     4021 tctttccctt ctggcaattt tctcaatgct ggtttcgccg ctggcgaccg cctacgctca
     4081 agggcttcct tcctccctgc gcacgaccgt gaacgacgtt gaacgcgaca acccgcgcgt
     4141 gttgcagatc atggagaagg cggagcagca ttacagactc ggcgaactca atctcaaaga
     4201 caagaacatg gacgcggcgc gcaccgagtt cgacaaggcg gttgattccg tcctcgaatc
     4261 cggcatggac gtgcgctcca atcccaaact ccagaccttc tacctgcaac tcgtcgagcg
     4321 cgtctaccgc atggaagtcg ccatgcctgc gccgacgcag aacagcggcc agcccgcgca
     4381 gtttgtcgcc gcttcgacga atgcgacgaa cgatgttgct gccgtcgccg cgcagcagcc
     4441 cggcttcctg cgcgagcaga agtttgaacc gtcgccgctc gacgagttga gccgtctcga
     4501 actgaccacc gaagagaagc aggtcacgaa cgaggacgtg gtcaaactcg aagaggccaa
     4561 gaacgccgtc gacttcgctt tcaattccaa caatttgatt cagggcttca tcaactacta
     4621 tcagggtcgc ggtcgcgcga cgatggagac gggcttgcgc cgttcgggtc gttacatttc
     4681 gatggcgcgc agaattttcc gtgaggaagg cgtgcccgaa gacatcgtct ggctcggcca
     4741 ggtggagagc gcgtggcgtc cgaccgctta ttcgtgggcg gcggcctccg gcctctggca
     4801 gttcattccg agcactggcg cggagtatgg cctgcgccag aacgcctgga ttgacgaacg
     4861 caacagcttt gagaaggcga cgcgcgcatc cgcccgttat ttgaagcatc tgaaccgtta
     4921 ctacaacggc aactgggaac tcgccctcgg cgcgtacaac acgggtccgg gcaacatcga
     4981 ccgtggcatc cggcgcggcg gcgcggccag tttctgggcg gcttacccct acatcgcgca
     5041 ggagacgcgc aactacgtgc cgaacatcct cgcgacgatc ctgatcgcga agaaccctgc
     5101 gaagtacggc ttccacaaca ttcgccccga tgcgccgctc gcttacgacg tggtcaacgt
     5161 gccttcggcg acgagcctgc aactgatcgc cagcgcgacg gacacgagcg ttgactatat
     5221 ccgcagcctc aatcccgaac tccggcgcga caccacgccg cgcggcgaaa gctacaacct
     5281 gcgcgtgcct cccggtcgcg gcaaatcctt cgtcgcgctc ttgaagcgca ttccgggcga
     5341 ccggcgcgaa tcggcgaaag ttatttcggt tgcgcccggc gaagacctcc aatcggtcgc
     5401 tgcccgcacg ggaacgagcg tggcgcagtt gcagatgtgg aacagtggcg ttgacctgaa
     5461 aggcggcggc aaggtcgtcg tcccggcagg ctcggtgcgc agcgtcgcga tggtgcgacc
     5521 gcgcggtaac gtcggcagca gtctgatctc ggtgcgcgct cgcgccggtg agacgctggc
     5581 gcaactcgcc gcacgtcagg gcgtcggcgc agacgaagtg gcacgcctca acgccatctc
     5641 gcccgatgcg ccgctctcgg ctaatcagga gatcaaggtt ccttcgaagt cgcccgcttc
     5701 cacgagcggc ggcacggctc ctgtgcggcg tcgcaggtaa gtcgaacgaa ggcggttcaa
     5761 gtgcaaaagg atgaaggatg aagttttgaa ttcatccttc atccttttgt tttattctcg
     5821 cccgcgcccc gtcagtctta ttttcgcccg cccctccgcc tcttaagttt cgcattgctc
     5881 gacacgtgcg cccgcaagtt taaaggcgcg cgggttcgcg cgtcctcact taagccatgc
     5941 tctttcgtac acagtcagcc gccgtctatg gcattgacgc agacctcgtt gacatcgaag
     6001 ttgacctgac gcccgcacgc aacgaggccg acacgcctgc gtccgtcacc atcgtcgggc
     6061 tacccgacgc cgccgtgcgc gagtcgcgcg agcgcatccg cgccgccatc aacaatagcg
     6121 cgttcttctt tccctttcac aagacaacga tcaatctcgc gcccgcagac gtgcgcaagg
     6181 aaggcgcgag ttttgatctg cccattgcgc tcggcatcct cggcgcaaac ggcgacctcg
     6241 gcgaacggga tcatctgacc gatgcgctct ccgtcggcga actctcgctc gacgggcgcg
     6301 tgcgcgcgat caagggcgcg ctctccatcg ccatccgcgc gcgcgacgcg ggcatcaggt
     6361 ttctgctgct gcccgaagag aacgcgacgg aggcggcggt cgtcgcaggc gtggatgtgt
     6421 accccgtgac ggacttgcgc gcggcggtcg aactcgtcgc cgcgctccgc aaaagcgacg
//
LOCUS       DASWNS010000445         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_406093, whole genome shotgun sequence.
ACCESSION   DASWNS010000445 DASWNS010000000
VERSION     DASWNS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406093"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VGB76_15320"
     CDS             complement(<1..252)
                     /locus_tag="VGB76_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100319.1"
                     /translation="MDGLECWTSLDDAVLRWRAAREGDNEMAGGWDGDALAGQMEDAL
                     ERQQAAAEARLRKNSSSPEELAREAQAESLRLSRARILDQ"
     gene            complement(271..621)
                     /locus_tag="VGB76_15325"
     CDS             complement(271..621)
                     /locus_tag="VGB76_15325"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEY0100320.1"
                     /translation="MKIILPESDEDLLRECEVDTFRASGPGGQHVNKTESAVRLTHLP
                     TGVVVTCREERSQHRNKAACLQKLRERVERLNHRPARRVPTRTTRAAKERTLEAKARR
                     SRLKRERARSGTDE"
     gene            complement(722..1885)
                     /locus_tag="VGB76_15330"
     CDS             complement(722..1885)
                     /locus_tag="VGB76_15330"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0100321.1"
                     /translation="MKAVAVFPEAREVKIVEQEEPRINAPTEVKLRVLEVGVCGTDKE
                     ICAFEYGTPPLGSPFLVLGHESLSEVVETGDAVSRLKVGDLVVASVRRPCPHDTCAAC
                     RMGRQDFCFTGDFTERGIKEQHGYMTEFVVDDERYMNVLPAHLREVGVLIEPLTIAEK
                     ALTQVWQVQQRLPWEVPMPGSGQKPAAPGVAAQTGAGGTQEWLHHRALVLGAGPVGLL
                     GAMALSVAGFDTHVYSREGATSDKARLVEAFGAKYLSAETHTVEGLAEAVGGLDLVYE
                     AVGASSLAFEVMKFLDTNGVFVFTGVPGRKAPVAVDTDLLMRNLVLRNQIAFGTVNAG
                     RDAFEAAIRDLDIFNTRWPQVVKSLITARVPIEQHRDLLLGKAGGIKNVVKLA"
     gene            complement(1895..>2999)
                     /locus_tag="VGB76_15335"
     CDS             complement(1895..>2999)
                     /locus_tag="VGB76_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100322.1"
                     /translation="SHALLNEAADAVQKDDEQFARYLRNRSRDLLSDDYESGDAAWIT
                     GRFKNLNAQIGSYEVYDDELYGVKAFYAFSLLATRQKETEDLRRAMKGIQALENSLPY
                     NRHKNVREDIPVGVYDVIADFGQSRGGNTATILPNENYLARRYGRTILLRANIMRHPE
                     IFKGSGNAWGAAVAPAHAGELTFDGSFYRTLWHEVGHYLGVDQTKDGRDLDTALQENS
                     SLLEEMKADLVSLYVGEALRKQGYYTDAGLRSLYASGIQRVLQNNRPRRTQPYNTMQL
                     MQWNFFLERGVLRFDPQTKRLSINYGKYHDAVGELLKRVLEVQYEGDRAVADRFVEQY
                     TNWDENLHGAVAQNIRQQQQYRFRIFKYAALGE"
BASE COUNT          506 a          992 c          887 g          614 t
ORIGIN      
        1 ttgatcgagg attcgcgctc tggagagccg tagagattcc gcctgcgcct cgcgcgcaag
       61 ctcttccggt gaagaactat ttttccgcag acgcgcttcc gccgccgcct gctgccgttc
      121 cagagcatct tccatctgcc ccgccagcgc gtcgccgtcc catccaccag ccatttcgtt
      181 atctccttcc cgcgccgccc gccagcgcag cacggcatca tccaaactcg tccagcattc
      241 aagcccgtcc atccgaacgt atcaacccgt tcactcgtcc gtcccgctcc tggcgcgctc
      301 acgcttcaga cgcgagcgac gcgccttcgc ttccagcgtg cgctccttcg cggctcgcgt
      361 cgtgcgcgtc ggcacgcgcc gcgccgggcg atgattcaga cgttcgacgc gctcgcgtag
      421 tttttgcaga caggcggcct tgttacggtg ctggctgcgc tcctctcgac aggtgacgac
      481 cacgcccgta ggcaggtgcg taagcctgac cgcgctctcc gtcttgttga cgtgctggcc
      541 gccggggccg ctcgcgcgga aggtgtcaac ctcgcactcg cgcaacaagt cttcgtccga
      601 ttccggcagt atgatcttca cgtcagaggc tcttcacatt aactcgaaca ttaatttgaa
      661 tggactgcta tctaagcgtc tgccctgtgc gacgctcgcg ccgacaatgc cccgtcgcgg
      721 ctcaggcgag tttgacgacg ttcttgatcc ctccggcctt gcccagcaaa agatcgcggt
      781 gctgctcgat gggcacgcgc gccgtaatca gcgacttaac aacctgcggc cagcgtgtgt
      841 tgaagatgtc cagatcgcgt atggcggcct cgaaagcgtc gcgcccggcg ttgaccgtgc
      901 cgaaggcgat ctggttgcgc agtaccagat tgcgcatcag caggtcggta tctacggcga
      961 ccggggcttt tcttccgggc acgccggtga agacgaacac gccgttcgtg tcgagaaact
     1021 tcatcacctc gaaggcgagg ctcgacgcgc cgaccgcctc atagacgaga tcgagtccgc
     1081 ccaccgcttc ggccagcccc tcgaccgtgt gcgtctccgc cgacaggtac ttcgcgccga
     1141 acgcttcgac cagccgggct ttatcgctcg tcgccccttc tctcgaatag acgtgcgtat
     1201 cgaaccccgc aaccgagaga gccatcgcgc cgagcaggcc gactggaccc gcgccgagta
     1261 ccagcgcgcg atgatgcagc cactcctgtg tgccgcctgc gcccgtttgc gccgcaacgc
     1321 cgggcgcggc gggtttctgc ccgcttcccg gcatcggcac ttcccagggc aggcgttgtt
     1381 gcacttgcca cacctgcgtc agagccttct ccgcaatcgt caacggttcg atcagcacgc
     1441 cgacctcgcg caggtgcgcg gggagaacgt tcatgtatcg ctcgtcgtcc acgacgaact
     1501 cggtcatgta gccgtgctgt tccttgatgc cgcgctctgt gaagtcgccc gtgaagcaaa
     1561 aatcctgcct gcccatgcgg cacgcggcgc aggtgtcgtg cgggcaagga cgacgcacgg
     1621 aagcgacgac gagatcgccc accttcaaac gactcaccgc atctcccgtc tcgacgacct
     1681 cggacagaga ctcgtggccg agcacgagaa aaggagaacc caaaggcggc gtgccgtact
     1741 cgaaggcgca aatctccttg tccgttccgc acacgccgac ttccagcacg cgcagtttaa
     1801 cctccgtcgg cgcgttgatg cgcggctcct cctgctcgac gatcttgact tccctcgctt
     1861 cgggaaagac ggcaactgct ttcataattt tcactcactc gccgagcgcg gcatatttga
     1921 agatgcggaa tctgtattgc tgctgctggc ggatgttctg cgccaccgcg ccgtgcaggt
     1981 tctcgtccca gttcgtgtac tgttcgacga aacgatccgc gactgcgcgg tcgccctcgt
     2041 actgcacttc gagcacgcgc ttcaaaagtt cgccgaccgc atcgtgatac ttgccgtagt
     2101 tgatggagag ccgcttcgtc tgcgggtcga agcgcagcac gccgcgttcg aggaagaaat
     2161 tccactgcat gagttgcatg gtgttgtaag gctgtgtgcg gcgcgggcgg ttgttctgca
     2221 acacgcgctg gatgccgctc gcgtagagcg agcgcaaccc cgcgtccgtg taataaccct
     2281 gcttgcgcaa cgcctcgccg acgtagagcg aaacgaggtc ggccttcatc tcttccagca
     2341 ggctcgaatt ctcctgcaag gccgtgtcga ggtcgcgccc gtccttcgtt tgatcgacgc
     2401 cgaggtagtg gccgacttcg tgccagagcg tgcggtagaa actgccgtcg aaggtgagtt
     2461 ctcccgcgtg cgcgggcgcg accgccgctc cccaggcgtt gccggaaccc ttaaaaattt
     2521 ccgggtggcg catgatgttc gcgcgcaaga ggatggtgcg cccgtagcgg cgcgcgagat
     2581 aattttcgtt cggcagaatc gtggcggtgt ttccgccgcg cgattgcccg aagtccgcga
     2641 tcacgtcata gaccccgacc gggatgtctt cgcgcacgtt cttgtgacga ttgtagggca
     2701 gcgaattttc gagtgcctgt atccccttca tcgcgcggcg caaatcttcc gtctccttct
     2761 gccgcgtggc gagcaggctg aaagcgtaga aggctttgac gccgtagagt tcgtcgtcat
     2821 agacctcgta agagccgatc tgcgcgttca gatttttgaa gcgtccagtg atccaggcgg
     2881 cgtcgcccga ctcgtaatcg tccgagagga gatcgcggct gcggttgcgc aggtaacggg
     2941 cgaactgttc gtcgtccttc tgcacggcgt ctgcggcttc gttcaggagc gcgtgggag
//
LOCUS       DASWNS010000446         7971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_146480, whole genome shotgun sequence.
ACCESSION   DASWNS010000446 DASWNS010000000
VERSION     DASWNS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146480"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2453)
                     /gene="uvrA"
                     /locus_tag="VGB76_15340"
     CDS             complement(<1..2453)
                     /gene="uvrA"
                     /locus_tag="VGB76_15340"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0100323.1"
                     /translation="MAIDRITVRGARQHNLKDINVEIPRDRLTVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLDQLERPDVDSVDGLSPAISIEQKTVSRSPRSTVGT
                     VTEVYDYLRLLFSSIGLPHCHICGAAITRQSVEQIVLSVLALPEGERVMILAPIVRGR
                     KGEFKKELEKLAKDGFLRARVDGELLSLDEEIKLDKRRNHTIEAVVDRLLIKPGINER
                     LTESVRTALKLTNGAVLVSVVDGEEKMYSERMACVNCGINIPPLEPRSFSFNSAYGAC
                     KRCHGLGTVLEVDPVKLVPEPTEQADKLIFLGAADKQGSAYLRSAVVAVAAHFGASAS
                     VPFSQLPKQAREAFFHGLEGQITFRQGLYTYQSDWKGAIRHLRERMNDAPSEKVREAL
                     EELVSPVECPACQGQRLQPESLAVRVGGLGITQYTALPIEEAVAVFDKLTLNERERQI
                     AGLVLREIRNRMRFLESVGLGYITLDRPSATLSGGEGQRIRLATQIGSQLRGVLYVLD
                     EPSIGLHPRDNQRLIETLCALRDLGNTVLVVEHDEETIRRADYVVDIGPAAGVHGGEI
                     VAVGTPEEVACNPNSVTGLYICGAQKIDLPEHRRAPNGHSVTVRGAREHNLKDIDVTF
                     PLGLLTVVTGVSGSGKSTLIDDILYRALARHLYGSLNEPGAYESIEGLDNVDKVIEID
                     QSPIGRTPRSNPATYTGLFTPLRELYAMLPESRERGYKPGRFSFNVKGGRCEACEGDG
                     LKRIEMNFLPDVYVLCDVCRGARYNRETLSVKYKGKSIAELLDTTVEEALPLLENIPQ
                     IKQKIQTLLDVGLGYIKLG"
     gene            2674..4614
                     /locus_tag="VGB76_15345"
     CDS             2674..4614
                     /locus_tag="VGB76_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100324.1"
                     /translation="MELSALRAVSVALEIPADKFRSKPVNPSSPRRLSTLALCLLFAA
                     QTTFTQTTGKTPKRNAANAAAPACDRQRALALVQQQAGEAKSFERPVGQITVMTRAAE
                     LLWPYNQEAARAVFADAFQLATTHFLAKGDEAKHEGRAAMIILPDQRVVVMRAIARRD
                     AEWARKLALEAAEDTRRAAETKAPQVLGVQSPGYKLLELASSLLTINQPVALELIRKS
                     FAYPANVTQARLFYELAPGNRTLADQLAREAITTYAQNGTTEDLSFISVYAFGLPGAI
                     SPLTMMMHYKVPQGFEPNAALQELALKAYVARAETILKTPEQYAENPSKFWPETMQML
                     VALTTLEPLAAQRFPAYAERISALKSSMHTANNEKEQQRAAEFLQGRRDHERESKFDS
                     LIEEIEGEATPEKKERLIANAAQVAETITQLEQVETLAQKIGESEARRQLSNWLNFRR
                     TQLLVKEGQLDEAKRVADRVDELDSRAILYFEIAREGIKKLEDKRRAHELLDEVATAA
                     GKAPNTPVKARTLLGITHLYAAFDALRAAEVMADAVKTINTLDDPDLSMGFILRRIEG
                     KNFSVYSGYNVPGFNLENSFHELGPRDFDGALSLARNLTDRAQRAIAILGLSAQCLKQ
                     TPKPGAPVKKEAKPGNSEKPVG"
     gene            4741..5499
                     /gene="pgsA"
                     /locus_tag="VGB76_15350"
     CDS             4741..5499
                     /gene="pgsA"
                     /locus_tag="VGB76_15350"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY0100325.1"
                     /translation="MNLPNALTLSRIFVVPLLVAVLLTPFSEDWLGVPRHLLAAGLFL
                     AAAFTDYLDGHIARRRQQVSRLGQLLDPIADKLLISAALISLVENRLAPAWAVVIIIG
                     RELAVSGLRSIAAADGLVIAASRMGKFKMGAQVLAIALLILSSVHGAPPVANFGQAFP
                     AIQVWSVPELREAVRHLLDGGATTATDWQVLLYTLGRATLWVVVLSACLSMYQYFRAF
                     YQSAVERAALEREALRAAKTTPPVGVEARTQTGG"
     gene            complement(5522..5989)
                     /locus_tag="VGB76_15355"
     CDS             complement(5522..5989)
                     /locus_tag="VGB76_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100326.1"
                     /translation="MPRCILTTILLLTLFVLVGVVASAALPNLTAGLAAKESSAVYHL
                     RTLAEAELKFKATTGKGRFGTMLELKEERLIDAELAGGLKDGYGFNVTTTSETFAVTA
                     APAKYPADGRRSFYFSLEDGIIRAADKQGLPADVNDPPLPESERIAASPGESR"
     gene            complement(5996..7015)
                     /locus_tag="VGB76_15360"
     CDS             complement(5996..7015)
                     /locus_tag="VGB76_15360"
                     /inference="COORDINATES: protein motif:HMM:NF027852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgT C-terminal domain-containing protein"
                     /protein_id="HEY0100327.1"
                     /translation="MHTPKHFSRLSGMAALLFVFCFALAPVEAQTPADSANETKTGAS
                     AVNAPAQMPAAVETELICGGYIEYAPNYNGFEIVGGEQEQEKPAYSVNDIVFINAGAQ
                     QGVKVGQEFQVVRPRGQFTTKLTHKSGWLGVYTQEIGRVRVTVVKENVSVAIITSECN
                     DLILLGDLLRGVPYRVSPPMGEIGDLDRFADPSGKQNGRIVLARDGREMLSRNQIVYI
                     DLGKEDNVKEGDRLTVYRPTGTGDISRFREEEVTPTANYGFESERFRGGKFSIKAQRV
                     EHPNRTGIHGPMVTTHKTERRRPAMPRKIVGEIVVLNVQARTATAVITRVAQEIHTGD
                     FVEVK"
     gene            7331..7406
                     /locus_tag="VGB76_15365"
     tRNA            7331..7406
                     /locus_tag="VGB76_15365"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7364..7366,aa:Thr,seq:tgt)
     gene            7428..7515
                     /locus_tag="VGB76_15370"
     tRNA            7428..7515
                     /locus_tag="VGB76_15370"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7462..7464,aa:Tyr,seq:gta)
     gene            7601..7676
                     /locus_tag="VGB76_15375"
     tRNA            7601..7676
                     /locus_tag="VGB76_15375"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7634..7636,aa:Gly,seq:tcc)
     gene            7722..7796
                     /locus_tag="VGB76_15380"
     tRNA            7722..7796
                     /locus_tag="VGB76_15380"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7754..7756,aa:Thr,seq:ggt)
     gene            7847..>7971
                     /locus_tag="VGB76_15385"
     CDS             7847..>7971
                     /locus_tag="VGB76_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0100328.1"
                     /translation="MSKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVLAKHNP"
BASE COUNT         1566 a         2461 c         2413 g         1531 t
ORIGIN      
        1 tgtccgagct tgatgtagcc gaggccaaca tcgagaagcg tctgtatctt ctgtttgatc
       61 tgcggaatgt tttcgaggag cggcagagcc tcttcgaccg tcgtgtccag cagctccgcg
      121 attgatttgc ccttgtactt caccgagagc gtctcgcggt tataacgcgc gccgcgacac
      181 acatcacaga gcacatacac gtcgggcagg aagttcattt cgatgcgctt caagccgtcg
      241 ccttcgcagg cttcgcaacg cccgcccttg acgttgaagg agaagcgccc gggtttgtag
      301 ccgcgctcgc gcgattcggg gagcatcgcg tagagttcgc gcaggggggt gaacagaccg
      361 gtgtaggtcg ccgggttgga acgcggcgtg cggccaatcg gcgactggtc gatctcgatc
      421 accttatcca cgttgtccaa gccttcgatt gattcatacg cgccgggttc gttgagcgag
      481 ccgtagagat gtcgtgcgag ggcgcgatag agtatgtcgt caatgagcgt tgacttgccc
      541 gaaccggaga cgcccgtgac gaccgtgagc aggccgagcg gaaacgtcac atcaatatct
      601 ttcaggttgt gttcacgcgc gccgcgcacg gtgacgctgt ggccgttcgg agcgcggcgg
      661 tgctccggca aatcgatctt ttgcgcgccg cagatgtaca gacccgtaac ggagttcgga
      721 ttgcaggcca cctcttccgg cgttccgacc gcgacgatct cgccgccgtg cacgccagcc
      781 gcggggccta tgtctacgac ataatcggcg cggcgaatcg tctcctcgtc gtgttcgacg
      841 acgagcacgg tgttgccgag gtcgcgcagg gcgcagagcg tctcgatgag acgttgattg
      901 tcgcgcgggt gcaggccgat ggaaggttcg tcgagaacgt agagcacgcc gcgcagttgc
      961 gagccgatct gcgtggcgag gcgtatgcgc tggccttcgc cgccggagag cgtggcggag
     1021 gggcggtcga gcgtgatgta gccgaggccg accgattcga gaaagcgcat gcggttgcgt
     1081 atctcgcgca ggacgagtcc ggcgatctgt cgctcgcgtt cgttgagcgt cagtttgtcg
     1141 aagacggcga cggcctcttc aatcgggagc gcggtgtatt gcgtgatgcc taagccgccc
     1201 acgcgcacgg cgagcgattc gggttgcagc cgttgaccct gacacgccgg acactcgacg
     1261 ggcgacacca actcctccag agcctcgcgc accttctccg acggcgcatc gttcatacgc
     1321 tcgcgcagat gcctgatcgc gcccttccaa tcgctctgat aagtgtagag tccctgccgg
     1381 aaagtgatct gtccttcgag gccgtgaaag aaagcctcgc gcgcctgttt aggcaactgc
     1441 gagaagggga ctgacgcgct cgcgccgaag tgtgcggcga cggcgacgac cgcgcttctc
     1501 aaataagccg agccttgctt gtcagccgcg ccgaggaaga tcagcttgtc ggcctgttcg
     1561 gtcggttcgg ggacgagttt cacggggtca acttcgagca ccgtgccgag tccgtgacaa
     1621 cgcttgcacg cgccgtaggc cgagttgaaa gagaaagagc gcggttcgag cggcgggatg
     1681 ttgatgccgc aattcacgca ggccatgcgc tccgaataca tcttctcttc gccgtcaacg
     1741 actgagacga gcaccgcgcc gttcgtcaac ttcaaagccg tgcggacgga ttcggtgaga
     1801 cgttcgttga tgcccggttt gatgagcaag cgatcaacga ccgcttcgat ggtgtgattg
     1861 cgccgcttgt cgagcttgat ctcttcgtcg agcgagagca gttcgccgtc cacgcgtgcg
     1921 cgcaggaaac cgtccttcgc cagtttctcc agttccttct taaactcgcc tttgcgcccg
     1981 cgaactatcg gcgcgaggat catcacgcgc tcgccttcgg gcagcgcgag cacgctcagg
     2041 acgatctgtt cgacgctctg gcgcgtgatg gccgcgccgc agatgtggca atgcggcagg
     2101 ccgatggagg agaagagaag tcgcaggtag tcgtaaacct cagtcaccgt gccgaccgtg
     2161 ctgcgtgggc tgcgcgagac ggtcttttgt tcgatggaga tggcgggcga gagtccgtca
     2221 accgaatcca cgtccgggcg ttcgagttga tctagaaact ggcgcgcgta ggccgacaag
     2281 gattcgacgt aacggcgttg gccttcggca tagatcgtat cgaaggcgag cgaagatttg
     2341 ccggaaccgg agagtccggt aatgacagtc aggcggtcgc gcgggatttc gacgttgatg
     2401 tctttgaggt tgtgctggcg cgccccgcgc accgtgatgc ggtcaattgc cattgcttaa
     2461 atctttgcca gtagagctac agacgtaaaa tttcgggagc gcagaaacag gtattttacg
     2521 cgagccgaca cagcagcgca agcaagcccg ccttgatgga ttttttctgc aacaaaagcg
     2581 aatctttgcc cgtcataccg tgcagtccgc cagtccgttc cgggagagtc gtcaaataat
     2641 ggaacctatc tgttccgaaa gagcaatcaa ctaatggagt tgagcgcgct gcgcgccgtg
     2701 agcgtggcct tagaaattcc cgctgataaa tttaggagta aacctgtgaa tccctcgtcg
     2761 cctcgtcgtc tctctacgct cgcgctctgt cttctctttg cagcgcaaac aactttcacg
     2821 caaaccaccg gcaagacccc gaagagaaac gccgccaacg ccgccgcgcc cgcgtgcgac
     2881 cggcaacgcg cactcgcgct cgtacagcaa caggccggag aggcgaagtc gttcgagcgt
     2941 cccgtcgggc agatcacagt gatgacgcgc gccgccgaac tgctctggcc ttacaatcag
     3001 gaagccgccc gcgccgtctt cgcggacgcg ttccaactcg ccaccacaca ttttctcgcc
     3061 aagggcgacg aggcgaagca cgaggggcgc gcggcgatga tcattctccc ggatcaacgt
     3121 gtggtcgtga tgcgagccat cgcccggcgc gatgcagagt gggcgcgcaa gctggcgcta
     3181 gaggcggcgg aagatacgcg gcgcgcggcg gagacaaaag ctccgcaagt gctcggcgtg
     3241 caaagccccg gctataagtt gctcgaactc gcctcgtcgc tcctcacaat caaccagccg
     3301 gtcgcgctcg aactcatccg caagagtttc gcctaccccg ccaacgtgac gcaggcgcga
     3361 ctcttctacg aactcgcccc cgggaatcga acgctcgccg accaactcgc gcgcgaagcc
     3421 atcacgacct acgcgcagaa cggcacgacc gaagacctct ccttcatctc cgtctacgcc
     3481 ttcggcctgc cgggcgcgat ctcgccgctc accatgatga tgcattacaa agtgccgcag
     3541 ggtttcgagc cgaacgccgc attgcaggag ttagccctca aagcctacgt ggcgcgcgcc
     3601 gagacgatcc tgaaaactcc cgaacagtat gccgagaacc cgagcaagtt ctggcctgag
     3661 acgatgcaga tgctcgtcgc gctgacgacg ctcgaaccgc tcgcggcgca acgcttcccg
     3721 gcctacgccg agcgcatctc agccctcaag tcatccatgc acacggccaa caacgagaag
     3781 gagcagcagc gcgcggcgga gtttctccaa ggccggcgcg accacgagcg ggagagcaag
     3841 ttcgactcgc tcatcgaaga gatcgaaggc gaggcgacgc ctgagaagaa agagcgtctg
     3901 atcgcaaacg ccgcgcaggt cgccgaaacg atcacgcaac tcgaacaggt cgagacgctc
     3961 gcgcagaaga tcggcgagtc ggaggcgcgc cgccagttgt cgaactggct caacttcaga
     4021 cgcacgcaac tcttagtcaa ggaaggtcaa ctcgacgagg cgaaacgcgt ggccgaccgc
     4081 gttgacgaac tcgactcgcg cgccattctc tatttcgaga tcgccagaga aggcatcaag
     4141 aaactggaag acaaacgccg cgcccacgaa ctgctggacg aagtggcgac ggcagccggt
     4201 aaagctccga acacgcccgt caaggcgcgc acgctgctcg gcatcactca cctctacgcc
     4261 gcgttcgacg ccctgcgcgc cgccgaagtg atggccgacg ccgtgaagac catcaacacg
     4321 ctcgacgatc cagacctcag catgggcttc atcctgcgtc gcatcgaagg caagaacttt
     4381 tccgtctata gcggctacaa cgttcccggc ttcaatctgg agaacagttt ccacgaactc
     4441 ggtccccgcg atttcgacgg cgcgctctcg ctcgcgcgca atctcacaga ccgcgcccaa
     4501 cgcgccatcg ccatcctcgg cctctccgcg caatgcctca aacagacgcc gaagccgggc
     4561 gcgcccgtga agaaagaggc gaagccgggg aactccgaga agcccgtcgg gtaagaccct
     4621 tgacaccttt tacgcgcggc tcctacactc tcgcggcaag aagctcatgt cgttcggagt
     4681 cgaggttgta ggccgtctcg caacccatgc gagacaaaag cgaaaagggg gttgaagagg
     4741 gtgaatcttc caaacgcgtt gacgctgtcg cgcatcttcg tcgtgccgct gcttgtcgcg
     4801 gtgctcttga cgccgttctc ggaagattgg ctcggcgtgc cgcgccacct cctcgccgcc
     4861 ggactttttc tcgccgccgc tttcacagac tatctcgacg gccacatcgc acgacgccgc
     4921 cagcaagtct cgcgtctcgg ccaactgctc gaccccatcg cagacaaact actcatctcc
     4981 gccgcgctca tctcgctcgt cgaaaaccgg ctcgcgccgg cgtgggcggt cgtcattatc
     5041 atcgggcgcg agttggccgt ctcgggtctg cgttcgattg cggcggctga cggcttggtg
     5101 atcgccgcct cgcggatggg caagttcaag atgggcgcgc aggtgttggc gattgcactc
     5161 ctgatcctgt cgagcgtgca cggcgcgccg cccgtggcaa acttcgggca ggcgtttccg
     5221 gcgattcagg tctggtctgt gccggaacta cgcgaggccg tgcgtcacct gctggacgga
     5281 ggcgcgacga cggccaccga ctggcaagtc ctgctctaca cgctcggacg cgcgacgctc
     5341 tgggtcgtcg tgctctcggc ctgtctctca atgtatcaat acttccgcgc cttttatcaa
     5401 tccgccgtgg aacgcgccgc gctcgaacgc gaagccctgc gcgccgcaaa gacaactccg
     5461 cccgtcggag tcgaagcgcg aactcagacg ggcggttgaa tgtctgtcaa ttgaatcggc
     5521 tttagcgtga ttcgccgggc gacgctgcga tgcgctccga ctcgggcagg ggcgggtcgt
     5581 tcacgtcagc cggaagacct tgtttgtccg ccgcgcgtat gatcccgtct tccagagaga
     5641 agtagaacga acgcctcccg tccgcaggat acttcgcggg cgcagccgtt acagcaaacg
     5701 tctcgctcgt cgtcgtcacg ttaaagccgt agccgtcctt gaggccgccc gccagttcgg
     5761 cgtcgatcag tctctcctcc ttcaactcca gcatcgtgcc gaagcgcccc ttgcccgtcg
     5821 tcgccttgaa tttaagttca gcctccgcga gcgtgcgcag gtgatagacg gcggaactct
     5881 ccttcgccgc aagccccgcc gtcaggttcg ggagcgcagc cgacgccacc acgccaacca
     5941 gcacgaagag cgtcagcaat aagatggtcg tcagaatgca gcgaggcata gcgttttact
     6001 tcacttccac gaagtcgccc gtgtgaatct cctgcgcgac gcgcgtgatt acggcggttg
     6061 ccgtgcgcgc ctgcacgttc aacaccacga tctcgccgac gatcttgcgc ggcatcgcag
     6121 gccgacgccg ttcggtcttg tgcgtcgtga ccatcgggcc gtgaatgccg gtgcggttcg
     6181 ggtgctcgac gcgctgcgcc ttgatggaga acttgccgcc gcgaaaacgc tcgctctcaa
     6241 aaccgtagtt ggccgtcggc gtcacctctt cctcgcggaa acgcgagatg tcgcccgttc
     6301 ccgtagggcg atagacggtg aggcggtcgc cttctttgac attatcttcc ttgccgaggt
     6361 ctatatagac gatctggttg cgcgacagca tttcgcgccc gtcgcgcgcc agcacgatgc
     6421 gcccgttctg tttaccgctc gggtctgcaa aacggtcgag gtcgccgatt tcgcccatcg
     6481 gcggcgagac gcgataaggc acgccgcgca gcaagtcgcc gagcaggatc aggtcgttgc
     6541 actcgctcgt gatgatcgcg acggacacgt tctccttcac caccgtcacg cgcacccgac
     6601 cgatctcctg cgtgtagacg ccgagccacc cggatttatg tgtgagcttc gtggtgaact
     6661 ggccgcgcgg gcgcacgacc tgaaactcct gcccgacctt gacgccctgt tgcgcacccg
     6721 cgttgatgaa aactatatcg ttgacgctgt aggccggctt ctcctgctcc tgttcgccgc
     6781 cgacgatctc aaatccgtta tagttcggcg cgtactcgat gtaaccgccg caaatcagtt
     6841 cggtttcgac agccgcgggc atctgtgccg gcgcgttgac ggcggacgcg ccggttttcg
     6901 tctcgttcgc ggaatcggca ggggtctgcg cctctaccgg agcgagcgcg aagcagaaaa
     6961 cgaaaagtag tgcggccatg ccgctcaggc gcgaaaagtg tttaggggtg tgcacggaac
     7021 ttctcctgaa tggttaaagg aaagtgaacg cttcggacaa agttggacaa ggctcaaaac
     7081 tgagccgcag aggctttgag tagcatctac acgggcgttg ggatgaggtg agagccgtgc
     7141 gggcgtctgt ggccgcacga cacaggacag ttcaatccta cttggtggcg gcgcagagcg
     7201 tcaacaaatt tcttcaaact gaccgccccg ccgttcgaaa tcagcgcaga ccctcgctgc
     7261 ggcttgcgtt ttacgggagc tattgttact attcgcgttt tgtcggaagt gctcggagcg
     7321 atccgtgtgt gccggcgtag ctcagttggt agagcatctg atttgtaatc agagggtcgg
     7381 gggttcgagt cccttcgccg gctccaacag atatttggac aaacagtgca ggggtggtcg
     7441 agtggttaat ggcaccgggc tgtaaacccg gcaggctaac gccttacgta ggttcgaatc
     7501 ctaccccctg caccagaaga tgaagaggct ggaggtcaga ggttagaggt cagttattgc
     7561 aactaacctc tgacctttag cctctgacct ctacgtttta gcgggagtag ctcagttggt
     7621 agagcatcag ccttccaagc tgagggtcgc gagttcgagc ctcgtctccc gctccacact
     7681 ttacgtgtgg ttaagggcgc aagcgcagca gttagagacg cgcccgcgta gctcaggggt
     7741 agagcgcctc cttggtaagg aggaggtcat gagttcaaat ctcatcgcgg gctccataca
     7801 aactgacggt ttacatcaca caacttttta aagaggctat ctcacaatga gcaaggagaa
     7861 attcgaccgg tcgaagccgc acgtcaacat cggcaccatc gggcacgtcg atcacgggaa
     7921 gaccacctta acggctgcga ttacgaaagt cttagccaaa cataacccca a
//
LOCUS       DASWNS010000447         3347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_406234, whole genome shotgun sequence.
ACCESSION   DASWNS010000447 DASWNS010000000
VERSION     DASWNS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406234"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..>3347
                     /locus_tag="VGB76_15390"
     CDS             455..>3347
                     /locus_tag="VGB76_15390"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100329.1"
                     /translation="MTLTPGKPVAASTARGTVQSFQVSLGSGQFLRASTSQEKSNLHV
                     TLYDPGGRRLSEFVCLRGETSHINFVAEVAGTYRFDVAPLGAEPDGRGYEFAVREIRA
                     GEERDQFRVAAAASQSEGARLLLEWREESLRRAVESYESGLRLWRQAGERGGEFYTLR
                     KLGEIRRSLGELKEALDFYQQALKLSREVRDAHGEAATLNDNGHVLLSLGRNEAAQDI
                     FQKSLRLSRAINDRREEARALNNIGEVFYALGKLDKSIEYYRQAQAICVETGDERGLA
                     LSLLNLGYSYSDLSETARALEFYEQAQRLWRQIGDRRNEAHTLTALGSLHAKSGEKQS
                     ALSLYEQALQLFLSMGEKVGRAFVLNGIGYVHFQLGETERSLEDYQQALTLLREAGYR
                     EGEANSLRSLGDISYTLGDMKAALAHYQQALDATRAMADQRLEPYVLKGIAAVYERLG
                     KQAEARGYYERALSLQRKGGDKREVAYTLNSLAHFDLDTGRGRAARARLEEALRLNRA
                     AADPSGESLTLYNFARFERVRGNLAEARAHAEASLGLVESLRTKVASMDLRASYFASI
                     HQRYEFYIDLLMSMHSAQPGAGFDALAFEASERARARSLLETLSEVRAGVRQGIDPSL
                     LERERQLQQTLNDKAERQMFLHGQQNREAAAALAKEIGQITTEYDSVRAQIRVANPRY
                     AALTQPRPLSLNQIQQQLLDDDTLLLEYSLGEKRSYVWAVTRAGLASYELPGRAEIEG
                     AARNLYELLVAHQPIAGETTEQRRARVTAAGEQYWNRARALAVMVLDPVAGHLSSRRL
                     LVVPDGALQYVPFEALPDPVAHAAGTQADVSADQHPFAPLILRHEIVNQPSASALALL
                     RGDTARRQQATRKAVAVLADPVFEHNDPRLKLASRGRPTSDDEPARPGETYVVLRDAG
                     MLSADGQLPRLLASRQEADSIVAAAASLESLKAVGFNASREMAMSPELSR"
BASE COUNT          673 a         1051 c         1035 g          588 t
ORIGIN      
        1 caacagcaac aacagcaaca acagcagcaa caacagcagg aaaacttaat gcctgaatcg
       61 ggtgcccggt gatatagtgt gcgccgcacg ggcatcccgt gcgcgggaga aacgagagac
      121 gacggacaga gacggctaca agccaagaag tatcccgctc cttgcaccag atgaagcgtc
      181 tgcgccgctt taagtcgtca tcacacgaca cgcggcagtc gccttcggtc ggcgcacacc
      241 gcaggctcag ttctttacgg tctgttaacc aacaggggag atgtgttcca tgaggatgag
      301 gggagaacga cgccgccccg cgtccggcgg gcgatgcagg gcggtgccgt cgcgcctgtt
      361 ggtattagct gtctcccttt attgccttat cccgcaactc tctttcctcc ctctcgaatc
      421 acacgctctc tccgcgactt cacacactga gtttgtgacg ctcacgcccg gcaagcccgt
      481 tgcggcttcg acggcgcgcg ggacggttca gtcgttccaa gtctcactcg gctccggcca
      541 attcctcagg gcatcaactt cacaggagaa gagcaatcta cacgtcacgc tctacgaccc
      601 cggcgggcgg agactcagcg aattcgtctg cctccgggga gagacgagcc acatcaactt
      661 cgtcgccgaa gtcgcaggca cctaccgctt cgacgtggct ccgctcggtg cggaacctga
      721 cggcaggggc tatgagttcg ccgtccgtga gattcgtgct ggcgaagagc gggaccagtt
      781 cagggttgcc gccgcagcca gccagtcgga aggcgcacgg ctgctgctcg aatggcgtga
      841 ggagtcgctc aggcgtgcag tcgaaagcta cgagagcggg ttgcgtctct ggcgtcaggc
      901 cggggagcgc ggcggcgagt tttatacgct gcggaaactc ggcgaaattc gacgttctct
      961 gggcgagttg aaagaggctc tagactttta ccagcaggca ttgaaactca gccgcgaggt
     1021 tagagacgcg cacggcgagg ctgcgaccct caacgataac ggtcacgttc tgctctcgct
     1081 cggacgcaac gaagcggcgc aggatatttt ccagaagtcg ctccggctaa gccgcgcaat
     1141 caatgaccgg cgggaggagg cgcgcgcgct caacaacatc ggcgaggtct tctacgcttt
     1201 aggcaaactg gataagtcaa tcgaatacta ccgtcaggca caggccatct gcgtcgaaac
     1261 cggagacgag cgcggcctcg ccctgtcgct cctgaattta ggctacagct attccgactt
     1321 aagtgagacg gcacgcgctc tggaattcta cgaacaggca cagagactct ggcggcagat
     1381 cggcgaccgc cgtaatgagg cgcacactct aactgccctc ggcagcctcc acgccaagag
     1441 cggcgagaaa cagtcggcgt tgagcctcta tgagcaggct ctccaactct tcctgtcgat
     1501 gggcgaaaag gtcggtcgcg ctttcgtcct gaacggcatc ggttacgtcc acttccaact
     1561 cggtgagacg gagagatcgc tcgaagacta tcaacaggct ctaacgctct tgagggaggc
     1621 aggctaccgg gagggcgaag ccaattcgct caggagcctc ggcgatattt cctacacgct
     1681 cggcgacatg aaggctgcgc tcgcgcatta ccaacaggct ctcgatgcca cccgcgcaat
     1741 ggccgaccag cgtttggagc cttacgtgtt gaagggcatc gccgccgtct acgaacggct
     1801 cggcaagcag gcggaggcac gcggctacta cgagcgcgcg ctctctctcc agcggaaggg
     1861 cggcgacaag cgggaggtgg cttatacgct taacagcctt gcccacttcg acctcgacac
     1921 gggacgcggg cgggcggcgc gcgcgaggct tgaggaagcc ctacgactta accgcgccgc
     1981 cgcagacccc tctggcgagt cgctgacgct ctacaacttc gcacgcttcg agcgcgtgcg
     2041 cggcaatctc gcggaggcgc gcgcgcatgc ggaagcatct ctcggtctgg tcgagtcgct
     2101 gcgcacgaag gtcgccagca tggacctgcg cgcctcctac ttcgcctcca ttcaccagcg
     2161 ctacgagttc tacatcgacc tgttgatgtc gatgcacagc gcgcagcccg gcgcaggttt
     2221 cgacgcgctg gcgtttgaag ccagcgagcg cgcccgcgcc cgttcgctgc tcgaaaccct
     2281 ctctgaagtg cgggccggcg tccggcaggg catcgatccg tcgttactcg aacgggagag
     2341 acaactacaa cagacactca acgataaggc cgaacgacag atgttcctgc acggccagca
     2401 gaacagagag gcggctgccg cgctggcgaa agagataggg cagattacga ccgagtacga
     2461 ttcggtacgg gcgcagattc gcgtcgccaa tccccgttac gctgcgctga cgcagccgcg
     2521 ccccctctct ctgaatcaga tacaacagca gttgctcgac gatgacacac tgctcctcga
     2581 atacagtctg ggcgaaaagc ggagttacgt ctgggctgtc acgcgcgccg ggctggcgag
     2641 ctacgaactg ccggggcgcg cggagatcga gggcgcggca cgtaaccttt acgaactgct
     2701 ggttgcgcac cagcccatcg cgggcgaaac cacggagcag cgtcgtgcgc gcgtgacggc
     2761 tgcgggagaa caatactgga atagagccag agcgctcgcc gtcatggtgc tcgatccggt
     2821 ggccggtcat ctcagtagca gacggctgtt ggtggtaccc gatggtgcgc tgcaatatgt
     2881 tcctttcgag gctttgcctg acccggtagc acacgccgca ggcacacagg ccgacgtatc
     2941 cgccgaccag catcccttcg ctcccttgat cctccggcac gagatcgtta accagccgtc
     3001 ggcctccgca ctcgcgttgc tgcgaggcga caccgcgcgc cgccagcagg cgacccgaaa
     3061 ggcagtcgcc gtgctcgctg atcccgtctt tgaacataac gacccacggc tgaaactcgc
     3121 cagccgggga aggccgactt cggacgacga gcctgctcga ccgggcgaga cgtatgtggt
     3181 tttgagagac gccgggatgt tgtcggctga tgggcagttg ccgcgcctgc tggcctcgcg
     3241 tcaggaggcc gattcgattg tcgccgccgc cgcctcgttg gaatcgctta aagcggtggg
     3301 cttcaatgcc agccgcgaga tggcgatgag tcctgaactc agccgtt
//
LOCUS       DASWNS010000448         5248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_68605, whole genome shotgun sequence.
ACCESSION   DASWNS010000448 DASWNS010000000
VERSION     DASWNS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68605"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VGB76_15395"
     CDS             complement(<1..196)
                     /locus_tag="VGB76_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100330.1"
                     /translation="MKSTGSRRKSLTVLIAMLFLVALALGGCRKESSSGGGGAASGGS
                     GQKGGKKVIGFSQMEMDGPWR"
     gene            complement(248..1921)
                     /locus_tag="VGB76_15400"
     CDS             complement(248..1921)
                     /locus_tag="VGB76_15400"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY0100331.1"
                     /translation="MHITELKSSLPSQTVRSVLRRTTFIVALFAACSAGVAAQQQSRT
                     NYTNPALAGDYPDPSVIRVGQDYWATATSSEWAPEFPILHSRDLVNWRVVGSVFKKRP
                     AWSVGNYWAPEISEDRGRFYIYYTAKKKDGPLCVAVATARRPQGPYTDHGALVCQEVG
                     SIDGFPIRDENGQRYLLWKEDGNSKSVPTPIWAQRLSPDGTRLVGERQELIRNDQPWE
                     KHASLPYGDLVEGPAVVRRGGWFYMFYSGNFCCARECNYQIGVARARKLLGPWEKNPA
                     NPILAGNDAFKCPGHGTIVSDARGRDYMMYHAMDAKDFVYVGRQALLDEVRWGANGWP
                     TINNGRGASTRAPSPHGFAERNAEHYFFDDFTAPVLQPGWQWPQANEPVMRTERVRGG
                     RLILSPAIEQTTSPAGAVVARTTTLGDYEATTLIDTRGMRRGALAGLSAYGDAENALG
                     AVVDGRNIILWRREKNEQKMVATINDAITSPLVHIRMTANNGRRYRFAISADGNRWRN
                     VGEEIDGEYLPPWDRGVRVALTAGGAAGAAGKFEWLRIAPTRKTNARGE"
     gene            complement(1972..3042)
                     /locus_tag="VGB76_15405"
     CDS             complement(1972..3042)
                     /locus_tag="VGB76_15405"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0100332.1"
                     /translation="MRIKDVAREAGVSTATVSHVINKTRFVSDETRERVLTAIERCGY
                     YPNAHARQLASGRSNTLGLIISDISNPFFPELVKSIETAAFERGYDLILSNTNYEAER
                     TSSYMQRLIERKVAGVALMTSELDTALVEKLARRRVCVVFLDLGSAGQCMNNIVVDYG
                     TGIEEAIVHLVALGHREVAFVGGPRHLRSAEKRLDAFRASLARHLPEASPQIYDGDFK
                     LEGGRRAARALMERGDEMPTAVVVANDMMALGVMQEFRECGVEVPRDVSIVGFDDIAF
                     AQLAAPPLTTVSLPREELGRRAVEVLIASIEHSEQQGTDIHISTRLVARASTARAPVR
                     PATDGDLNVETKPRDGANVIRS"
     gene            complement(3169..4356)
                     /locus_tag="VGB76_15410"
     CDS             complement(3169..4356)
                     /locus_tag="VGB76_15410"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY0100333.1"
                     /translation="MIDRAALRRAFQELYGTSQGEAVRLFRAPGRVNLIGEHTDYNDG
                     FVLPMAIDRETCVAARPRHDRRVRLYSLNVDERVEFDLDAPGERERGIWLDYIEGIAQ
                     ALERRGVRLRGADLALSSDVPVGAGLSSSAALEVSSGLALASVAGIEVDRIELALAGQ
                     EAEHTYVGAKVGIMDQFIAALGQAGHALLIDCRTLETKAIPVDTTETVIAICDTRVKH
                     ELSASEYNTRRAECEQGVELLRGAGLEGIRALRDVSAADLQKYESALPEVVGRRCRHV
                     VTENARTLAAADALAGGRLAEMGQLMLASHASLSRDYEVSCRELDLLVDIAMSLGGGA
                     TLGARMTGGGFGGCTVNLVRRAALAEFTGTITREYTEAIGQPPGIYVSEAGAGAREVT
                     SDE"
     gene            complement(4353..>5248)
                     /gene="galT"
                     /locus_tag="VGB76_15415"
     CDS             complement(4353..>5248)
                     /gene="galT"
                     /locus_tag="VGB76_15415"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEY0100334.1"
                     /translation="PAVEASELYPVRPGQGICEVLVYTPNHNSTLAQEPVEQIYKLIQ
                     VWTDRFRELSAREFAKYVFIFENKGEAIGVTLHHPHGQIYAYPFIPPRLERELALARG
                     HMDETRRCLLCDILHEERSDGRRIVAENEAFAAYIPFFARYPYEVHVTSTRHLQALTD
                     LDADEQRALASILKTVLVAYDKLFNLSFPYMMVLHQRPTDGANYEHYHFHIEFYPPLR
                     TATKLKYLAGSETGAGLFINDTLAEEKAAELRGHVEPVVWQTRRADDSGDGSSSTNNS
                     GDKAGDKPLTTPPQPREETSL"
BASE COUNT          899 a         1675 c         1665 g         1009 t
ORIGIN      
        1 tgcgccacgg cccgtccatc tccatctgcg aaaagccgat gaccttcttg ccgcccttct
       61 gcccgctgcc tccgctcgcc gcgcccccgc cgccgctact gctctcttta cgacaaccgc
      121 ccaaagctag cgcgaccaga aacagcatcg ctatcaagac cgtcaaagac tttcgcctgc
      181 tgcctgttga tttcatgttt caccttctct cgtgatttaa gtttccgcgc gatatagaaa
      241 ttttcgtcta ttcgccccgc gcgttcgtct tgcgcgtggg cgcgatgcgc agccattcaa
      301 actttcctgc cgcgcccgcc gcgccccccg ccgttagcgc gacgcgcacg cccctgtccc
      361 acggcggcag atactcgccg tcaatctcct cgcccacgtt gcgccaacgg ttcccgtccg
      421 cgctgatggc gaaacgatac ctgcgcccat tgttcgcagt catgcgtatg tgaacgagcg
      481 gcgacgtgat ggcatcattg atggtcgcca ccatcttctg ttcgttcttc tcgcgccgcc
      541 acaggatgat gttgcgcccg tctacaactg cgccgagcgc gttctccgcg tcgccgtagg
      601 cggagaggcc ggcgagcgcg ccgcgacgca tcccgcgcgt atcgatcaga gtggtcgctt
      661 cataatcgcc gagcgtcgtc gtgcgcgcga cgaccgctcc ggcggggctg gtcgtctgct
      721 caatggcggg cgagagaatg agacgaccgc cgcgcacgcg ctccgtgcgc atgacgggtt
      781 cgttcgcctg cggccactgc cagccaggct gtaagacggg cgcggtgaag tcgtcgaaga
      841 aatagtgttc ggcgtttcgt tcggcgaagc cgtgcggcga gggcgcgcgc gtcgaagcgc
      901 ctctgccgtt gttgatcgtc ggccagccgt tcgcgcccca acgcacttcg tcgagcaacg
      961 cctgccgccc gacgtagacg aaatccttcg cgtccatcgc gtgatacatc atgtagtcgc
     1021 gcccgcgcgc gtctgaaaca atcgtgccgt gaccggggca cttgaacgcg tcgttgcccg
     1081 cgaggatcgg gttcgcgggg ttcttctccc agggtcccag gagtttgcgc gcacgcgcga
     1141 cgccgatctg gtagttgcac tcacgcgcac aacagaagtt gcccgaatag aacatgtaga
     1201 accacccgcc gcgccgcacg accgccggac cttcgaccag atcgccatag ggcagcgagg
     1261 catgtttctc ccacggctga tcgttgcgaa tcaactcctg ccgctcgccg acgaggcgtg
     1321 tgccgtccgg cgagagccgc tgcgcccaga tgggcgtcgg cacgctcttg ctgttgccgt
     1381 cctctttcca caagagataa cgctgcccgt tttcgtctct gatcgggaag ccgtcaatcg
     1441 agccgacttc ctgacagacg agcgcaccgt gatcagtgta tggaccctgc ggtcttcgcg
     1501 ccgtggcgac ggcgacgcag agcggcccgt ccttcttctt cgccgtgtag tagatgtaga
     1561 agcgaccgcg atcttcggag atctccggcg cccagtaatt gccgaccgac cacgcgggac
     1621 gcttcttgaa gaccgagccg acgacgcgcc agttgacgag atcgcgcgag tgcaggatgg
     1681 gaaactccgg cgcccactcg gaagaggtgg ccgtcgccca gtagtcctgc ccgacgcgga
     1741 tcacggacgg gtcggggtag tcgcccgcga gcgcgggatt cgtgtaattc gtgcgcgact
     1801 gctgttgtgc cgcgacgcct gccgagcacg ccgcgaagag cgcgacgatg aaggttgtcc
     1861 ggcgcagcac gcttctgacc gtttgtgacg gcagcgaaga tttgagttct gtgatgtgca
     1921 tttttagatt gaccctcgtt ccatacaaaa atggctccgg acgcttttcg attaactccg
     1981 aatcacgttc gcgccgtcgc gcggctttgt ctcgacgttc aagtcgccgt ccgtcgccgg
     2041 gcgcacgggc gcgcgggcgg ttgaagcgcg tgcgacgagg cgcgtcgaga tgtgtatatc
     2101 cgttccctgc tgctccgaat gctcgatgct ggcgatgagc acttcgacgg cgcggcgtcc
     2161 gagttcttcg cgcggcaggc tcacggtcgt caagggcggc gcggcaagct gtgcgaaggc
     2221 gatgtcgtcg aagccgacga tggacacgtc gcgcggcact tccaccccgc actcgcgaaa
     2281 ctcctgcatc acgccgagcg ccatcatgtc gttggcgacg accacggcgg tcggcatctc
     2341 gtcgccgcgt tccatcaagg cgcgcgcggc gcgtcgcccg ccttcgagtt tgaagtcgcc
     2401 gtcgtagatt tgcggcgagg cttcgggcag gtggcgcgcg agcgaagcgc gaaaagcatc
     2461 gagccttttc tccgccgaac gcaggtggcg aggcccgccg acgaaagcga cctcgcgatg
     2521 accgagcgcg acgagatgca cgatggcctc ctcgatcccc gtgccgtaat ccacgacgat
     2581 gttgttcatg cactgccccg cgctgccgag atcgagaaag acgacgcaga cccgacgccg
     2641 cgccagtttt tcgacgagcg cggtgtcgag ttccgaagtc atcaaggcga ctccggccac
     2701 cttgcgctcg atcaaacgct gcatgtagct cgaagtgcgt tcggcctcat agttcgtgtt
     2761 cgacaggatc aggtcgtagc cgcgttcgaa ggccgccgtc tcgattgatt tgacgagttc
     2821 gggaaagaac gggttcgaga tgtcggagat gatcaggccg agcgtgttgc tgcgccccga
     2881 agcgagttgg cgcgcgtgcg cgttcgggta gtagccgcac cgttcgatag ccgtgagcac
     2941 acgctcgcgc gtctcgtccg acacgaacct cgtcttgttg atgacgtgtg agacggtcgc
     3001 cgtcgaaacg cccgcctccc gcgccacatc tttaattcgc atgatcaaac agtgacaagt
     3061 gacaagtgac gggtgacaag ttaagacagt gacgagcgac gggtgatgag tgacgagtta
     3121 aaacagaaga aatcttgtct ttaagtttgt ctttcatctt gtcactcgtc actcgtcact
     3181 ggtcacttcc cgtgctcctg cgcccgcctc cgacacgtag atgccgggtg gttggccgat
     3241 ggcctctgtg tattcgcgcg tgatcgtccc ggtaaactcc gcgagcgcgg cgcggcggac
     3301 gaggttgacg gtgcagccgc cgaagccgcc gcccgtcatg cgtgcgccga gcgtcgcgcc
     3361 gccgccgagc gacatggcga tgtcaaccag cagatcgagt tcgcggcaac tgacttcgta
     3421 gtcgcggctg agcgaagcgt ggctggcgag catcaattgc cccatctctg cgagccgccc
     3481 gcccgccagc gcatcggcgg cggcgagcgt gcgcgcgttc tccgtcacca cgtggcggca
     3541 gcgtcgcccg accacttcgg gcagcgcgct ttcgtacttc tgcaaatccg ccgcgctcac
     3601 atcgcgcagc gcgcggatgc cctctaagcc cgcgccacga agcaactcca cgccctgttc
     3661 acactccgcg cggcgcgtgt tgtactcgga ggcagacagt tcgtgcttca cgcgcgtatc
     3721 gcagatcgca atgaccgtct cggtcgtgtc aaccgggatc gctttcgtct ccagcgtgcg
     3781 acaatcgatc aggagcgcgt gacccgcctg tccgagcgcg gcgatgaatt gatccatgat
     3841 gccgaccttc gcgccgacat acgtatgctc cgcttcctgt ccggcgagtg cgagttcgat
     3901 ccggtcaact tcgatgcccg cgacggaggc gagcgcgagt cccgaagaga cttcgagcgc
     3961 ggcggaagag gacagccccg cgccgacggg aacgtccgaa gagagagcga gatcagcccc
     4021 gcgcaggcgc acgccgcgtc gttctaaagc ctgcgcgatg ccctcgatat agtcgagcca
     4081 gatgccgcgc tcgcgttccc ccggcgcgtc gaggtcgaac tccacgcgtt cgtccacgtt
     4141 cagagagtag aggcgcacgc gtcgatcatg gcgcgggcgg gcggccacgc acgtctccct
     4201 gtcaatcgcc atcggcagca cgaagccgtc attatagtcc gtgtgctcgc caatcaagtt
     4261 gacgcgaccg ggggcgcgaa acaggcgcac ggcctcgccc tgcgacgtgc cgtacagttc
     4321 ttgaaaggcg cgccggagcg cagcgcggtc aatcaaaggc ttgtctcctc ccgcggttgc
     4381 ggcggcgtcg ttaacggttt gtcacccgcc ttgtcgccgc tgttgttagt gctgctactg
     4441 ccgtcgccgc tgtcgtcagc gcgtcttgtc tgccagacaa ccggctcgac gtgcccgcgc
     4501 agttcggcgg ccttctcttc ggcgagcgtg tcgttgatga acaagcctgc gcccgtctcg
     4561 ctgcccgcca gatatttgag tttcgtcgcc gtgcgcagcg gcgggtagaa ctcgatgtga
     4621 aaatggtagt gctcgtagtt tgcgccgtcc gtgggtcgct ggtgcaagac catcatgtag
     4681 gggaacgaga ggttgaagag tttatcgtaa gcgacaagga cggtcttgag tatggaggcg
     4741 agcgcgcgtt gctcgtccgc atcgagatcg gtcaaggcct ggaggtggcg cgtggaagtg
     4801 acgtgcacct cgtaagggta gcgcgcgaag aaagggatgt aggcggcgaa agcctcgttc
     4861 tccgcaacga tgcgccgccc gtcgctccgc tcttcgtgga gaatgtcgca gagcaggcaa
     4921 cggcgcgtct cgtccatgtg accgcgcgcg agcgcgagtt cgcgttcgag gcgcgggggg
     4981 atgaaggggt aagcgtaaat ctggccgtgc gggtgatgaa gcgtaacgcc gatggcctcg
     5041 cctttgttct cgaagatgaa gacgtacttg gcaaactcgc gcgcgctgag ttcgcggaag
     5101 cggtcggtcc agacttgaat gagtttatag atctgttcga cgggttcctg cgcgagcgtc
     5161 gaattatggt tcggcgtata gacgagaact tcgcagatgc cctgaccggg gcgcaccggg
     5221 taaagttccg aggcttcgac ggcgggcg
//
LOCUS       DASWNS010000449        19915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_432955, whole genome shotgun sequence.
ACCESSION   DASWNS010000449 DASWNS010000000
VERSION     DASWNS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19915
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432955"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VGB76_15420"
     CDS             <1..822
                     /locus_tag="VGB76_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100335.1"
                     /translation="QAQVQMEAVWGGQPRGKLPATALSKSNCGIRVYLEQFLGIRFPA
                     TIESLYPRFFVTVRLALETIVRRAQGENRRVTSDEAEQIFARAFPDDEPLGHAHYKIY
                     RRKGAAYAARFARAYEPQPHALEFFDTEDLINRAEDELLPLRLDLVTYYRGEAGQAHA
                     ILFRPESFEKKSKNGTELAWSSVEGTTRKRMSFVLLRKLDANLRPWIYSGEDGKIYEY
                     KWNSRNPTAMDDDAAWAKGRLKAFSRGRFETVVDEFICDTRCRCRVPCPFWMKAI"
     gene            936..1259
                     /locus_tag="VGB76_15425"
     CDS             936..1259
                     /locus_tag="VGB76_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100336.1"
                     /translation="MSEERTQGQDKDKDKGKQPRVTLQVQTPRGLWSRTAPADAAKRP
                     VYEISTKVAQVLTDARAVFKFTESDNKYVLLQGSAALDPERPLASYHFADDTLLVLSV
                     QGGNA"
     gene            1259..1717
                     /locus_tag="VGB76_15430"
     CDS             1259..1717
                     /locus_tag="VGB76_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100337.1"
                     /translation="MSVEPAVSLDAITTELADAVPYAASAGLALDVSALSEGDLRFYV
                     TFYNRAGVKFYAEFDCRDYPLYPPTIEFTDETRTARGMPSLYPGGFHPTPCVCMRYNR
                     KAYTERGGPHGDWRLVDWHLATPGGGPIETLAMIISDMHTKILDSPGRMG"
     gene            1717..2319
                     /locus_tag="VGB76_15435"
     CDS             1717..2319
                     /locus_tag="VGB76_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100338.1"
                     /translation="MKNLMGRLKSMMSRKKVKTREELLQPVVYITDTLLAETGRLIAS
                     FAEGSESEGVVYWFGFELGDKAVVTTLIVPNANTSHGCISTSPGANGEALGVIVGTPL
                     VLLGQAHSHPGERVRHSPVDDRETFARFDGGLSVVVPHFARRGVDLSRCGVHRHVDGA
                     FKFIRPERVGEHVVVVPGQADLRRREQEGRIRKEKANVVG"
     gene            2306..3784
                     /locus_tag="VGB76_15440"
     CDS             2306..3784
                     /locus_tag="VGB76_15440"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY0100339.1"
                     /translation="MSLVELSEINRRWATPELFYAERDNRTMLEVQDLARYAARPVEI
                     RVDPREAHELAVQRMTLLACNLTARWAREIRVVLPLGVRLASCLRRDGWDTLDLRVIS
                     EMQAADPFGRFMVLSPGEVRRSDLIERPLRLRIGSWREPLPVGEEISADDYVITASGW
                     SAIGRRGRGLGDEDRGHSSAVSAAAGLAASIGVADIFKRAIGHGREHWVTDFAWCMWS
                     HTLTQDVPARIKDSHVSEELDLGQTLLAGVGAIGSALVYLMDMMDLRGGLTLLDRDRV
                     ELSNLNRSPLFFVAHVLNDELKTATAANYLRRHELQVRVVDGTWHEHAREVAREVYDV
                     WVSFTNEDGAWAEVPFQLPPIVLHGTTTSGWGFGAGRHIPRRDDCTLCRMPRPEAEFR
                     GPCAQGEIESELEGEPARASLPFLSAASASLVLAELSKLSSPEVLKLPNDIAADLRYG
                     LPAVMALTRLANQACRGCRAAASNLWERRGGRGRYRPYSEAA"
     gene            4006..4563
                     /locus_tag="VGB76_15445"
     CDS             4006..4563
                     /locus_tag="VGB76_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100340.1"
                     /translation="MEAFETLVAALLERKGYWTRVSVKVELTKEEKRKIGRPSAPRWE
                     LDVIAYKAATNELYVVECKSYLDSTGVKLSSFDGSNEKGAARYKLFNDLTLRRVVFNR
                     LSLQLVEAGFCPSKPKITLCLAAGNTYQDASLLRQHFERNDWVLWDGDWVRRELAELS
                     KSGYDNTVAAIVSKLLLRPPKTTIK"
     gene            4757..5517
                     /locus_tag="VGB76_15450"
                     /pseudo
     CDS             4757..5517
                     /locus_tag="VGB76_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(5893..6399)
                     /locus_tag="VGB76_15455"
     CDS             complement(5893..6399)
                     /locus_tag="VGB76_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100341.1"
                     /translation="MTDSPLDLKLPYEEFYDYIPYPKSLVCWGFDIEELTKSNKYQFT
                     SSVYKAMLKGFGELAKSKNKRARERHIPVLCGYIIWNFWIGVPEGVPDAAFNILNRHL
                     TQVTGYKSLNVGEAESFGLSFRFAYDRGRVIQYLDESVAGEKRRNAYGSGVTVNYRLL
                     LDWGVAPG"
     gene            complement(6879..7316)
                     /locus_tag="VGB76_15460"
     CDS             complement(6879..7316)
                     /locus_tag="VGB76_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100342.1"
                     /translation="MVRKSRGSEIIGAIRKYLIDAAKQPEPITDERLMEVADCARATF
                     YKYVTEGSEIERDIKTARAEQQRYMELKDTSEDEIRLEPSLRERLERAEAGTRELLAL
                     FARMTDNLLRLGIPVEIIQAAQREAMTHPKRNFSHAGKGRRGK"
     gene            complement(7496..10402)
                     /locus_tag="VGB76_15465"
     CDS             complement(7496..10402)
                     /locus_tag="VGB76_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0100343.1"
                     /translation="MKVEMDSMTNEPPFLPVLPELPTFLTLQDGKQYDVSGDVWSFTQ
                     GGGIKLSVKFNWTLLANIVVAGTSTRVMSERAVTLVKLYVLERLDTGGDSIKPLTGKR
                     CEYAMVAFARFLAAYPMWMPAGRSFDWSDLSAEILDAWLTLENQKKQKREYAGLVRQF
                     YLWGADPAAGRPDFSTDLASELSAIRIKRHARGELVESRDIKRGPFNREELELMFSAC
                     ATGKGEAQDRAIVLTLLETAMRPKQLYLLTNQDLEVSKGVSEVDDADGTLTEVSYRLR
                     VRKIKLRRNVIKYHVLPLSACCGKLLNDLRKPGSNADDPLLWWINSSFWDSITTRLKA
                     FSEDADLRSPRIPIEYPEDEGPTHERLHLTPRRFRYAVATDRIAYGDSPTDVADMLGH
                     KSKKDVGVYIETSPWIADYFQKATDYAIRPLVAMMEGRAKPSEMNLLADIAPPVAPQL
                     RIYTDAPLFMGDSGSHSHSNNQRMNMPSAMPVRPATKPGKSEAKIKDLVARARRKFPL
                     IYPGQDFDAQLWKVVHLRERQNVNQVVSFGFTTRDSTVSTRLSTRPEDALPPYFAAVV
                     KSWMVISNEVTVATNADRLYAARNFWHFLATRQGGNAASFRWGDLCESDMRAFEQFLM
                     SSESNRKQSLGPGSVLKIIQKMQRLVDFLASHGICRRIDYIPQTQAPRLAATRQLEAK
                     RLAAEMKLPAPGVLDTLASIYHRLTTAPEGEVSDWVLIFISGVAILMLTGLRIGELVT
                     LPYDCEVEEKRPKADPGELDSYWYGIKYWVEKRGRKTMRIKWISPTAEPIVRASIVRV
                     KRLTAAARERARVLEADPMRVPLPPELESRSTLSGPELRAIIGQKDKKRVHDDPQGLL
                     PQHGSCRWTHYYVKDLGPYLLSRRVTELYTIRHDDGTVQKLSESLFIIFAKQSRKTRG
                     GTLSSSGRAGEVASVLDVFVITSHYLQGLWKRRVAEGVICKPAQFPPLADAYCL"
     gene            complement(10402..11682)
                     /locus_tag="VGB76_15470"
     CDS             complement(10402..11682)
                     /locus_tag="VGB76_15470"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0100344.1"
                     /translation="MWVVERLDVDGEELPILRHAEMWLPAPIALRYALWARFRVGPSS
                     LTNDFRAIAILYNWAEATEGIGYFEDFLTSGRVLTRDQLLAFLPYLQSRRYIDADELI
                     TLSPDYITLPPIVGNQTYNARLVAVREFIGWTVEPVNHSGDMLFDEDERELKLAKMAR
                     LCNEHTLTVGESLRPDPITKEEIRLIRKAIMPDELGNYPPNGFRKATRFRNWIMFEVA
                     LNLGLRKGELLTLKVSHLPALIDLLQFFFVPRQQDAPEDPRKRRRLRGKTNERRVPLM
                     EPDLLPSILGYRDAAPPIGRNDPRITSPYFFVTSEGQPIACSTADHIIKQIGKYAARL
                     VDEDMTLDEHVRARLKESLLALSWHRLRHTWAEQAAQKLYLKYGVGAWAILKEWGGWN
                     SEESMERYIEYAKRAISEQAAREYLSSFSKKGKH"
     gene            complement(12915..13511)
                     /locus_tag="VGB76_15475"
     CDS             complement(12915..13511)
                     /locus_tag="VGB76_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100345.1"
                     /translation="MTDWRDVKEARMRLALGLMLEEIGKYECAADVLRATIACDPQLV
                     EAHVWLGIIYEGMARYEETVGEFVEAIRIDERAARMAVSTEPEEITRIRQILNAWPVT
                     PSPIPEEETWYPPDLPPQIREASELSEAASKHIGAGQDAEAVEALERAVRLDPVSRPY
                     AALLAFAYVLNRQQRVVWASRSVLWEVSPGLARVVFKH"
     gene            complement(13554..15302)
                     /locus_tag="VGB76_15480"
     CDS             complement(13554..15302)
                     /locus_tag="VGB76_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0100346.1"
                     /translation="MARKVLPSVVYIEMEGVGGKPGCYGSGFFVTQTQILTNKHVVTC
                     SEARRGTVKLAGGQRSYPTKWIVAWPDLDVALVETEGLTAQPLQLDTVRQLSVGDDIY
                     VVGNPEGLEGTFTRGILSGVRSQGGILQIDAPVSPGSSGGPVVDAYGRVVGVTVSSIR
                     EGQNLNFAIPARLLAFPLKQMQGMLARVRKEGAPPAAADNSARTPAAPHAAPPGKAAP
                     PSPARRTWEANHDWADFVSKTLGDSVVKDELKALLDSGLDVNATDRGGRTALHLAATL
                     GQAELARYLLSRGADINARNIPGRTPLMLAVGPYDFKLPGGGYAPLGDFWSAPPCGEV
                     GDAKPAMYETSWPTWYSVAEDRRPLILALLNAGADLTAADRQGLTAFDHAAAGGLTGF
                     ERLLHEPGKTGLPPACDLRVKDAPALRGLRLGMSVSEVSARLGGLRPTPGRCGLSTLY
                     LDGRQLSAAARGFEGVALVRLIFLDDRVVYLNVGYGREFPFKSFDEYLSTLSSSLGLP
                     GMWRRVAGAPDFEQAHAMTCEGFVVAAGRIGSLYVELHDTEAVRTLVRRDIEAGQRLK
                     QEAEREAERRRRSFKP"
     gene            complement(15479..15997)
                     /locus_tag="VGB76_15485"
     CDS             complement(15479..15997)
                     /locus_tag="VGB76_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100347.1"
                     /translation="MSRGEPDTVIVEKHYRMTLDFRVLVGDVRKDGVERAGDDEEGAD
                     EHLERQRRLLRALLRDERALDEFMTYLIIDRVCDHPDSELGMVFGVRTEEEILEPVFS
                     ALGEDDAQFFREVRRDGILWDNTEQFEFCFAVDWTGATLIEIAAKKEGDTSAAEAGQA
                     YLRRFTHRGDKS"
     gene            16562..16879
                     /locus_tag="VGB76_15490"
     CDS             16562..16879
                     /locus_tag="VGB76_15490"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0100348.1"
                     /translation="MGSAARRKPARLAEKLLAIRSSLGLSQNGMIRRIGFVDELTQAE
                     ISMFERGIRVPSLPVLLEYARAANLYLDVLVDDSLDLPLRIPAPAKHEGIKRKSNYSK
                     RAK"
     gene            17096..18217
                     /locus_tag="VGB76_15495"
     CDS             17096..18217
                     /locus_tag="VGB76_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100349.1"
                     /translation="MESVMGDERRSEMTQGELLSEMHRLGYDDVTERQLADWRRKDLL
                     PPFDVTGGGRGQRRGRERSSWTDGESVLNQALWVRELMQIYRSTESIRLPLWVLGYPV
                     PLKRVREALGGPLNEITNEIAEAIENEARAGGEIEDMIEEVTYHHFEEMRREGAEALL
                     LPQHSLEAFFNVFFNQDYDLTDGAFELGIEELREYETAMRQRQDAALAAEGFSDTEPA
                     REDDSLLRLVDRAPFIKQYFSLHQLKQAVDECSDSDLRAVQRDLSYLRGMTQLLRKII
                     TILTREIPPEYHPSRADSLRPILSVGGLIILADLSLRRHGFAREIDYFLPEALRRFRQ
                     QFTEEMERELVEASKQIPEIIETCIPIIVNTFLQESKVG"
     gene            complement(18477..19319)
                     /locus_tag="VGB76_15500"
     CDS             complement(18477..19319)
                     /locus_tag="VGB76_15500"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0100350.1"
                     /translation="MSKVWFITGAGRGMGVDITKAALAAGYKVVATGRNTDKVTQAVG
                     ESADLLVVKLDVTNPADAEAAVKAAVDRFGRIDVLVNNAGNFYAGFFEEISPEDFRAQ
                     IETNLFGPVNVTRAALPVMRAQRSGLVVMISSIAGIIGQEFVSAYCASKFGVEGWMES
                     LTPEVAPFGIRTMLVEPGFFRTELLTEESTKYPQPSIDDYAEKTRQTVTAWKGMNGLQ
                     GGDPAKLAKALIQLASQDEPPLRWAAGADAVQAVEQKAKDLLAQADAYRELSLSLAHD
                     DTSA"
     gene            complement(19496..>19915)
                     /locus_tag="VGB76_15505"
     CDS             complement(19496..>19915)
                     /locus_tag="VGB76_15505"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY0100351.1"
                     /translation="RVLLKDFPTAKFVTMIYAILDPNKGTMTFASAGHLSPLLIDSKG
                     ATFLETDAGLPLGMFECEFSEHEIQMPAGSRLFLYSDGITEANNSSFDEYGAVRLHDH
                     VSNPSASVKSLLDDVNTFATGFPATDDITVVLVKARE"
BASE COUNT         4278 a         5679 c         5582 g         4376 t
ORIGIN      
        1 caggcgcagg ttcagatgga agctgtgtgg ggcggtcagc cgaggggcaa acttccggcg
       61 acggctctca gcaaaagcaa ctgcggcatt cgtgtctacc ttgagcagtt tctcgggatc
      121 aggttcccgg caacgattga aagcctctac ccccgattct tcgtgacggt gcggctcgcg
      181 ctggaaacaa tcgtgcggcg ggctcagggt gagaaccggc gggtgacgtc ggacgaagcc
      241 gagcaaatct tcgcgcgcgc cttccccgac gacgagcctc tcggtcacgc ccactacaag
      301 atttaccggc gcaagggcgc ggcgtacgcc gcgcgcttcg cacgggcata tgaaccgcaa
      361 ccgcacgcgc tcgaattttt cgacacggaa gatttaatca acagagccga agatgagtta
      421 ttgcccctga ggctcgatct ggtgacttat tacaggggcg aagcaggaca agcgcacgcg
      481 atcctattcc gccccgagtc gttcgagaag aaatccaaga atgggacgga actcgcgtgg
      541 agcagcgtcg aaggcaccac gcgtaagagg atgtctttcg tgctgctcag gaaactcgac
      601 gcgaatttgc gaccttggat ctactccggt gaggacggga aaatttacga gtacaagtgg
      661 aatagccgta accctacggc gatggacgac gacgcagcgt gggctaaggg gcgtctcaag
      721 gcattctcgc gcgggcgttt cgaaacagtc gtcgatgagt tcatatgcga cactcggtgc
      781 cgctgccgcg tcccctgccc gttctggatg aaggcgatat gaaaaactgg taaaagcaca
      841 aaatgtagtg cacatgggac aacgactgtc cgtatatagg tgtaggggcg gaataagccg
      901 tctctacact ttttactttt tgggagacaa aagacatgtc agaagagaga actcaaggtc
      961 aggataagga caaggacaag ggcaagcagc cccgcgtcac gctgcaggtg cagacgccac
     1021 gcgggctgtg gagcaggacc gccccggcgg acgccgcgaa gcgccccgtc tacgagattt
     1081 cgacgaaggt agcgcaggtc ctcaccgacg cgcgcgcggt cttcaaattc acggagtcgg
     1141 acaacaagta cgtgctcctc cagggctcgg cggctctcga cccggagcgc ccgcttgcca
     1201 gttaccactt cgccgacgac acgctgctcg tcctgtcggt gcaggggggg aacgcctgat
     1261 gagcgtcgag ccggcggtgt cgctcgacgc cattaccacc gagctggcgg acgcggtccc
     1321 gtacgccgcg tccgccggac tcgccctcga cgtctcggcg ctcagcgaag gcgacctgcg
     1381 cttttacgtc accttctaca accgggcggg cgtcaagttt tacgccgagt tcgactgccg
     1441 ggactacccg ctttacccgc cgacgattga gttcacggac gagacgcgga cggcgagggg
     1501 catgccctca ctctacccgg gcggcttcca cccgaccccg tgcgtctgca tgcgctacaa
     1561 ccgtaaagct tatacggagc gcggcggtcc gcatggggac tggcggcttg tcgactggca
     1621 cctcgccacg cccggcggcg gtccgattga gacgctggcg atgattatca gcgacatgca
     1681 cacgaagata ctcgactcgc cagggaggat gggctgatga aaaatttgat gggaagactt
     1741 aaatcgatga tgagtcgcaa gaaggtaaag acgagggagg aacttctgca gcccgtggtc
     1801 tacatcaccg acacgctgct ggcggagacg ggaaggctca tcgctagttt cgccgaaggc
     1861 agtgagtcgg aaggcgtggt ctactggttc ggtttcgagt tgggcgataa agccgtcgta
     1921 acgacactga tcgttcccaa cgccaacacc tcacacggat gcatttcgac ctcgccgggg
     1981 gcgaacggtg aagctcttgg cgtcatcgtc ggaaccccgc tggtgctgct cggtcaggcg
     2041 cattctcacc cgggtgaacg ggtgcgccac tcgcccgtcg atgatcggga gactttcgct
     2101 cgtttcgacg gcgggttgtc tgtggtcgtc ccacatttcg ctcggcgcgg cgtcgacctc
     2161 agtcgctgcg gcgttcaccg gcacgtcgac ggcgcgttca agttcattag accggagcgt
     2221 gtgggcgaac atgtcgtggt agtcccgggt caggctgatt tgcggcggcg cgaacaggag
     2281 gggcggattc ggaaggagaa ggccaatgtc gttggttgag ctgagtgaaa ttaacaggcg
     2341 gtgggcgacg cccgaactct tctacgctga gcgcgacaac cgcacgatgc tcgaagttca
     2401 agacttggct cggtacgccg cccgacccgt cgagattcgc gtcgacccgc gtgaggcgca
     2461 cgagctagcg gtccagcgga tgaccctgct ggcgtgcaac ctgacggcgc ggtgggctag
     2521 ggaaatacgc gtggtactgc cgctgggcgt gaggctcgcg tcgtgcctga ggcgtgacgg
     2581 atgggacaca cttgatttga gagtcatcag cgagatgcag gcggcagacc cgttcgggcg
     2641 ttttatggtg ctttcgcccg gcgaagtccg ccggagcgat cttatcgaga gacccctgcg
     2701 cttacgcata gggtcttggc gcgaaccgct gcctgtcggc gaggaaattt cagccgatga
     2761 ctatgtcatc accgcgtcgg gctggtcagc aatcgggagg cgcgggcgcg gtctcgggga
     2821 cgaagaccga gggcactcga gcgccgtctc cgcagccgcc gggctcgcag catcaatcgg
     2881 cgttgctgac attttcaagc gcgccatcgg gcacggccgg gagcactggg tgacagactt
     2941 tgcgtggtgt atgtggtcac acacgctgac tcaggacgtt cccgccagaa tcaaagattc
     3001 acatgtgtcg gaggaactgg accttggcca aacgctactt gctggagtgg gtgccattgg
     3061 ctccgctcta gtgtacttga tggatatgat ggatctgcgg ggtgggctaa ccctgctcga
     3121 ccgtgaccgc gtagagttgt cgaacctcaa ccgctcgccg ctgttcttcg tggcacatgt
     3181 cctcaatgac gaattaaaga ccgcaaccgc tgccaattac cttcggcggc acgaactcca
     3241 ggtgcgcgtt gtagacggca cgtggcacga gcacgctcgc gaagtggcaa gagaggttta
     3301 tgatgtctgg gtcagcttca ctaacgaaga tggcgcgtgg gcggaagttc ctttccaact
     3361 gccgcccatc gtccttcacg ggacaacgac cagcggatgg gggttcgggg cggggcggca
     3421 catcccgcgt cgcgacgatt gcacgttgtg taggatgccg cgccctgaag cagaatttcg
     3481 aggtccgtgc gcccaagggg aaatagagtc agaactcgaa ggggaaccag cacgcgcctc
     3541 actacctttt ctgtcagccg cgtccgcttc actagttctg gctgaactct caaagctgag
     3601 ctcgcccgaa gttctgaagc tgccgaacga tatcgctgcc gatctgcgct acgggctccc
     3661 tgcagtaatg gctcttacgc gattggcgaa ccaagcctgt cgcggctgca gggcggcggc
     3721 gtcaaatctg tgggagaggc gcggcggccg cgggcgttac cgcccctatt cggaagcggc
     3781 gtgagattca gttattgaga tggcgaaatg aaagtgatgg tgaacaagcc gaactgtcgg
     3841 gtgaaggctc accaccactt tcgtacgcgt cttcttcgcg ctgcgtcaca tcaagccgcg
     3901 aaggcccctg accgaagatc ggttcttcga cgaagaagac ctcaggcaga cgtatttgat
     3961 agaagatatg ttttaggcgt agcagaccgc cgagagagga aatgcatgga agctttcgag
     4021 acgttggtag ccgcgctgct tgagcgcaaa ggctactgga ctagagtgag cgtcaaggtg
     4081 gagttaacca aagaggagaa gcgcaagatc gggcgccctt cggctccgcg atgggaattg
     4141 gacgtcattg cctacaaagc ggcgaccaac gaattgtatg tggtcgagtg caaatcatac
     4201 ctagactcca ccggcgtcaa gttgagttcg ttcgacggga gtaacgagaa gggcgccgca
     4261 cgctacaagc ttttcaacga cctgacctta cgccgcgtcg ttttcaaccg gctgtcgctt
     4321 cagctcgtgg aagcaggatt ctgcccatcc aaaccgaaaa taacgctatg cctagctgca
     4381 ggcaatacat accaagacgc gagcttgttg cggcaacatt ttgagcgaaa cgactgggtg
     4441 ctctgggacg gcgactgggt gcgccgagag ctcgccgaac tctctaaatc aggctatgac
     4501 aacactgtgg cagctatcgt cagcaagcta ttgttgcgtc ccccgaagac gacaattaaa
     4561 taggtcttga cttaaagcta acgcctatta cacgtcttaa ttacggctat gcttccgatt
     4621 gttctccgca ttgatgtatg gagggttgct ataatgaaga taggttcgac tccaagtgag
     4681 caatccgttc gtttgcagcg tccaatgctg ccttcagctt gctaggttct gttgacattt
     4741 cgattaagct gttcagatga gaacagtaaa acaaacgaac gagcaatggg aaaagatact
     4801 gccgttttta cgttcatccg aacaggtcta tgtcggcgac gaagccgagt gtcgatgttt
     4861 ccttgaagct gtgctgtgga tcactcgctc cggcgcacag tggcgattgt tgccagatgt
     4921 ttacggtcac tggaacacca tttacaagcg ctttgccaga tggagcgaac gcggcatctt
     4981 cgagcaactg catcagcact tcattgatga accggatatg caacacctga ttctcgactc
     5041 gaccattgtg cgtgctcatg cggccgccgc cggagcgcaa aaaaaagcgg cggggaggaa
     5101 gcgcaagcct taggccggag tcgtggcggc ttttccacca agattcactt gagcgttgat
     5161 gcgctcggcc tgccactgag atttcgcttg acgggtggtg agcgtcacga tattacgcaa
     5221 gcggcggcac ttgtcgaagg tgacgacttc gagatgctga ttgccgatac gagttacgat
     5281 tcggcagact tcatcaaaag tatcaaagaa caaggcgcgg aagcggtcat tccgtcacgt
     5341 cgcaaccgca cagaacagcg tgagattgat cgtcacacat acaaagaacg ccatctggtc
     5401 aagtgcttca tcggcaaggt caagcattac cggcgcatct tctcacgctt cgacaaactg
     5461 gctaacagat atctgagctt cttgagtttt gttgctgcac tcatttggct tcgctaacaa
     5521 tgtcaacaga acctagcttc ccgtaatgcg gacatctcac gcttagacga cccctacttg
     5581 ttattcgact cttcgccgct ctcattcccg ctctctgatt ccagcgcgct taactgtttc
     5641 aggccgaggt tgtctaggat tacatctcgt gaagcgtcct ccacggcatg ccggtcacgg
     5701 taggcccgtt ccatgaccat taccgtgtct ccgaaatatt tactggcaga ctgggatgaa
     5761 ccgccgtggt cgagcacgaa agtgtccatc gaagcgccgc gtagatagtg tggatggaga
     5821 gcaggatgac ctgctttgaa agttcagttc cttttgtgca ggctccggag cactggcatg
     5881 ccgctttcca cctcaccccg gggctacgcc ccagtcaagt aacagccggt agttgactgt
     5941 cacccctgag ccatacgcat ttcggcgctt ctcgccggct accgactcgt ctaagtactg
     6001 gatgacacgc ccccgatcgt aggcgaatct aaaacttaat ccgaacgatt cggcttcccc
     6061 tacattcaac gatttgtagc ccgtcacctg cgttaagtga cgattcagta tgttaaacgc
     6121 ggcgtcgggc acgccttccg gtactcctat ccagaaattc catattatgt aaccgcacaa
     6181 taccgggatg tgacgttcgc gcgcgcgttt gttcttggac tttgccagtt ccccaaaacc
     6241 ttttagcatg gccttgtaaa cgctcgacgt gaattgatac ttgttggatt tcgtcagctc
     6301 ctcgatgtcg aagccccaac agactaatga cttagggtaa gggatgtaat cgtaaaattc
     6361 ctcataaggc agtttgaggt ccaagggtga gtcagtcatg ttcctttaat ctcctttcaa
     6421 gattcattct tccggcctca catctttgag catgaacatt cgtgagagtt gctcgataag
     6481 ttcctggtca gacatccgat agcggctagc cgtgtgccgt ctacgatctt ggcggcgaga
     6541 cctttgagtt ctccagtttt tagtcagact acatatgagc cgcaagttca tatcaatcta
     6601 tgtagatttg ttaataaggt gagaaggtaa taactctaat tgattgatag tgcttgaaat
     6661 ttgcatatgt gtagaaaatg aaagtgaaca ctttggaatt tataagtgaa cagtgtaaaa
     6721 tgccacctga gcgataagcc gtctgttgtc acagtttaca aagcgaacat ttgatatcat
     6781 agtgcaaaac tctgcaaaag cactatattc caaaatgtgt tcggattaac catgtttagc
     6841 actaacgggt tcgtcataat tgagccttcc tgtcacagtt acttgccccg gcgacccttg
     6901 cctgcatgac taaaatttct tttcggatgc gtcatcgcct ctcgttgcgc cgcctgaatg
     6961 atctctactg ggataccaag cctaagtaag ttatccgtca ttcgtgcgaa gagtgccagc
     7021 aactcccgtg tacccgcctc cgcccgttcg agtctttcgc gtaagctcgg ctcgagccta
     7081 atctcgtctt cactcgtgtc cttcaattcc atataacgct gctgttcagc gcgagctgtc
     7141 tttatgtcac gctcgatctc cgagccttcg gttacgtact tatagaacgt agcccgcgcg
     7201 cagtcagcca cctccatcag ccgttcatca gtaatgggct caggttgttt agcggcgtcg
     7261 atcaggtact tacgaatagc acctataatc tcactaccgc ggcttttccg aaccaatgtg
     7321 tttctcctgc acccgccctt cagagacact aggcgggacg taaaaacgct caaccctcaa
     7381 ctcctcggga gcgtaagcgt cggtttcgtt acttgaaaaa tgaaggtaat ctagcgcgct
     7441 ggaaatatag agcttagcga agtagcgtag aagtagatgt atgtccatcc ccccttcata
     7501 agcaatatgc atcagccagt ggcggaaact gtgcgggttt gcagataact ccttctgcca
     7561 ctcttcgttt ccatagtccc tgaagataat ggctggtaat gacaaatacg tcgagaacgc
     7621 tcgcgacttc accggctcta ccagaagaag acagtgtccc cccccgtgtc tttctcgact
     7681 gtttggcgaa gatgatgaag agtgactcgg aaagcttttg tacagtgccg tcgtcgtggc
     7741 ggatagtgta aagctccgtg accctcctcg acagtaggta tggtcctaag tcttttacgt
     7801 agtaatgagt ccaacggcaa ctgccgtgct gggggagtag accttgcgga tcatcatgga
     7861 cccgcttctt atctttttgg cctatgattg cgcgcagttc cggcccggat agagtgctcc
     7921 gactctcaag ctcaggtggc agaggcaccc tcattggatc ggcctcaagg actctagccc
     7981 gctcccgtgc agccgcggtc agtcgcttga cccgcacgat tgacgcgcga acaatgggtt
     8041 cggccgtcgg cgaaatccac ttaatgcgca tcgtcttcct tcccctcttc tcaacccaat
     8101 atttgattcc gtaccagtac gaatccagct cgccggggtc ggctttcggg cgtttctcct
     8161 ccacctcaca gtcgtatggc aacgtaacca actcacctat gcgcaacccc gttaacatta
     8221 aaatcgccac cccggaaatg aagatgagca cccaatcact tacttcccct tcgggtgcag
     8281 tcgtaaggcg gtggtaaatg ctcgcgagtg tgtcgagaac gccgggagca ggtagtttca
     8341 tttcagccgc gagcctcttt gcttcaagct gacgcgttgc tgccaagcgc ggtgcttggg
     8401 tctgcgggat gtaatcaata cggcgacaga tgccgtggct ggcaagaaaa tctactagcc
     8461 tttgcatctt ctggatgatc tttagtactg agcctgggcc gagtgattgc ttccgattcg
     8521 actcgcttga cataagaaat tgctcaaagg cgcgcatgtc actttcgcac agatcacccc
     8581 acctaaagga cgcagcgttt ccgccctgcc gcgtagctag gaaatgccag aaattccgcg
     8641 cggcatagag tcggtctgcg ttggtcgcta ccgtgacttc attcgagatt accatccagc
     8701 ttttaacaac tgctgcgaag tatggcggga gagcatcttc cgggcgagtt gaaagtcggg
     8761 tacttacagt cgagtctcgc gtggtgaacc cgaaactcac cacctggtta acgttttgac
     8821 gctctctgag atggacaact ttccataatt gcgcgtcgaa atcttggccg gggtagatta
     8881 gagggaactt gcgacgcgcg cgggcaacca gatctttaat cttggcctca gacttacccg
     8941 gttttgttgc ggggcgcact ggcatagccg agggcatgtt catcctctga ttgttactgt
     9001 ggctatgact ccccgaatcc cccatgaata gcggtgcgtc agtgtaaata cgtaactgtg
     9061 gagctacagg cggggcaata tccgcaagca aattcatctc tgacggcttg gcacgtccct
     9121 ccatcattgc caccaatggc ctaatggcgt agtccgtcgc cttctgaaaa tagtcagcga
     9181 tccacggcga agtttcaatg taaacaccga catctttttt acttttatgc ccgagcatat
     9241 cggccacatc cgtgggacta tccccatacg cgatgcggtc tgttgctacg gcgtagcgga
     9301 agcgacgtgg cgttaagtgc aagcgttcgt gagtagggcc ttcgtcttcg ggatactcga
     9361 taggaatgcg gggggagcgc aggtcagcat cttcagagaa ggccttcagt ctagtagtta
     9421 tgctatccca gaacgagctg tttatccacc ataaaagcgg gtcatcggca ttactcccgg
     9481 gtttgcgtaa atcatttaac aacttcccac agcacgctga caggggcaga acatgatact
     9541 taatgacgtt acgacgcagc ttaatctttc gcactctcaa tcggtaacta acctcggtca
     9601 gtgtcccatc tgcatcatcc acttcactca cacccttgct gacttccaaa tcttggttgg
     9661 tcaacaggta gagttgtttc gggcgcatgg cagtctcaag taatgtcaac acgatagccc
     9721 ggtcctgcgc ctctcccttg ccggtcgcac aggcgctaaa cattaattcc agttcttcgc
     9781 ggttaaacgg accacgcttt atatcgcggc tttcaaccag ttcgcctctg gcatgacgtt
     9841 taatgcgtat ggcgctaagc tccgacgcca aatcggtgga aaaatcaggg cgtcccgcag
     9901 ccgggtcagc cccccataag tagaattgtc gcaccagtcc agcgtattct cgcttctgtt
     9961 tcttctgatt ctctaaggtt aaccatgcgt ctaaaatctc ggcggacaaa tcactccagt
    10021 cgaagctacg acctgcaggc atccacatag gatatgcagc caaaaagcgg gcgaaggcta
    10081 ccatggcata ctcacagcgc tttccggtca gtggtttgat cgagtctcca ccggtgtcca
    10141 atctctctaa gacgtaaagc ttgaccagcg ttacggcccg ttcagacatc acgcgcgttg
    10201 aggtgcccgc aactactatg ttggcgagaa gcgtccaatt aaatttcacg gataacttga
    10261 tgcccccgcc ttgagtaaaa gaccatacat cgccagagac gtcgtactgc tttccgtctt
    10321 gtagtgttaa aaacgttggc agttccggaa ggactggcaa gaagggtggc tcattggtca
    10381 tgctatccat ctcgactttc atcaatgttt gcctttctta ctgaaggacg acaaatactc
    10441 acgcgccgct tgctcgctaa ttgcgcgctt ggcatactcg atgtatcgct ccattgactc
    10501 ctcactgttc caaccgcccc attccttgag gatggcccac gcgccgacgc catacttgag
    10561 ataaagcttt tgcgctgcct gctccgccca ggtgtgacga aggcggtgcc acgagagcgc
    10621 gagcagactc tccttcagcc gtgcccgcac gtgctcatca agcgtcatgt cctcatccac
    10681 gaggcgtgct gcatacttac cgatctgctt gataatgtgg tcggcggtac tacatgcgat
    10741 cggctggcct tcgcttgtca cgaagaagta gggtgaagtg atccttgggt catttctacc
    10801 gatgggcgga gcagcatccc tgtagccgag tatcgaaggc aagaggtcag gctccatgag
    10861 cgggacacga cgctcattcg tcttcccgcg gagccgccgc ctcttccgcg ggtcctccgg
    10921 cgcatcttgc tgtctgggca cgaaaaaaaa ttgtagtaaa tcgatgagcg ccggcaggtg
    10981 gctgaccttt agggtcagca gttccccctt tcttaagccg aggttgagag ccacctcgaa
    11041 catgatccag ttcctgaagc gcgtggcctt gcggaagcca ttcggtgggt agttgccaag
    11101 ctcgtcaggc ataatggcct tacgaatgag ccgtatctcc tctttagtga ttgggtctgg
    11161 tcgaagggac tctcccaccg tcagcgtatg ctcattgcat agacgcgcca tctttgccaa
    11221 cttcagctca cgttcgtcct cgtcgaagag catgtcaccg ctgtggttca caggctcgac
    11281 agtccaccct atgaactcac ggacggcgac gagcctagcg ttgtaagttt gattgccgac
    11341 gataggtggt agcgttatgt aatccggcga gagggttatc agttcatcgg catcaatgta
    11401 acgccgactc tgaagatatg ggaggaatgc cagcagctga tccctggtta agacccgtcc
    11461 cgatgtgagg aagtcttcga agtagccgat tccttcggta gcctctgccc agttgtacaa
    11521 gatggcgatc gcccgaaagt cgttcgtgag ggaagagggt cccacccgaa agcgagccca
    11581 tagtgcatat cgtaaggcga tcggtgccgg tagccacatt tccgcgtggc ggaggatcgg
    11641 caactcttcg ccgtcgacgt cgaggcgctc tacgacccac atcgtcggca cctcttgagg
    11701 gaaattcgtt gtcctaaaca aagcccgccg cccccgcaac ttggaataca tgcctgaaga
    11761 ggggagctca atcgctccca aacttcagcc gtagccgcgg tctgctcgtc gggccattcc
    11821 tgacgcaatg ccggatggtc catgttgctc cttggaaagc aacatctacc cgtcggagag
    11881 gagtcccctc aaggcagctc cgccgctact gggcattcga attgcttcga gataagggcc
    11941 aacgtggaaa ctctgtcgga cctcgatgtg cgggtgcgcc ctgcgttggt ggatgcagag
    12001 cggtgctttg gacggtgggc tctactctgt cggtagatgc cgtaggctta atttgttatg
    12061 ccgcactatt agcattgcgg cgcatcttat ctttcttgat aagaaatggc aagtggtaat
    12121 tagataaatt gtcagagccg cagttcattg gatgaaatgt gttgtgagtc acgcatgcgc
    12181 cccgtttcag ggacagcctc cggagccggc tagagtgcgc cggagttcta caacacgacc
    12241 tctttagtcc gacactgcta aggatggtct ttggcccaag cagcaatcac ccaatcgctc
    12301 tctgtcgata tccctcctct cttttgctga ttcgtgtatc aggcgacatg caaaactctc
    12361 ctgagattta cagatacgac ttcaatcttc ggatgcgaaa ccatgaacct acgtgaactc
    12421 aaacgcaccg ttagctccct ggctccgaac caactcgtca ggctcgacgc ctggctccac
    12481 gagttaatca atgctacgca gtccgggaag cactaccttc gtgccgcggc caaacgtgaa
    12541 gggctgatag ggcgtcaaga cgatcacaag acgtaccacc tcaaaggcgt gcgctgcggg
    12601 aaagagaact gccgatgcac ggtcggtgaa ctccacggcc cttattggta cgcctactgg
    12661 tctgaaggcg gcaagaccag atcacagtac gtcggtaagg aactccctga cgcgaccaag
    12721 acgaagcaga ggtctagccc gcgcagagtc cgataattct tatctaagac ttcgagtgtg
    12781 ttagataaga ttatcagacg taacgaatct ctccccactc ccttcctctg agtgtgagat
    12841 tgccagtgtg ctctcccact tcctgtagat tagttccgcc aaccacacct cccaccgcac
    12901 atcttcgtag ggactcagtg cttgaagacg acgcgcgcca accccggcga gacttcccat
    12961 aacaccgacc tgctggccca caccactcgc tgttgcctgt tcagcacgta ggcgaacgcc
    13021 agcagtgccg cgtaaggccg cgacaccgga tcaagtctca cggctctctc caacgcctcc
    13081 accgcttccg cgtcctgccc cgcaccgatg tgcttactcg ccgcctccga cagctccgac
    13141 gcttcccgga tctgcggcgg caagtccggc ggataccacg tctcctcctc agggataggc
    13201 gagggcgtca ccggccacgc gtttaagatc tggcggattc tcgtgatctc ttccggctcg
    13261 gtactgacgg ccatacgtgc tgcccgctcg tctatccgga tggcttccac gaactccccg
    13321 actgtctcct cgtagcgcgc catcccttcg tagatgatgc ccagccacac gtgcgcctcc
    13381 acgagctgcg ggtcgcaggc aatggtcgcc cgcagcacgt cggcggcgca ctcgtatttc
    13441 ccgatctctt cgagcatcag cccgagtgcg agcctcatgc gcgcttcctt cacatcccgc
    13501 cagtcggtca ttctcgtctc gttctcctaa tgttatggca aatgacggct cgctcatggc
    13561 ttgaatgacc gcctgcgccg ctccgcctct cgctccgctt cctgcttcag tctctgcccg
    13621 gcctcaatgt ctctgcgcac gagcgtccgc acggcctccg tgtcatgcaa ttccacgtac
    13681 aggctgccga tgcgcccggc agccacaacg aacccctcgc acgtcatcgc gtgcgcctgt
    13741 tcgaagtcgg gggcgcccgc cacccgtcgc cacatgcccg gcagcccaag tgaggacgac
    13801 agcgtggaga ggtattcgtc gaagctcttg aaggggaact cccgcccgta gcccacgttg
    13861 aggtagacga ccctgtcgtc gaggaagatc agtcgcacca gggcaacgcc ctcgaagccc
    13921 cgcgccgccg cagacagttg cctgccgtcg aggtagagcg tcgagaggcc gcaacgcccg
    13981 ggcgtcggtc gcagccctcc gagacgggca gagacttcgg agacgctcat cccgaggcgc
    14041 agcccgcgca gggccggcgc gtccttcact ctcaggtcgc aggcgggcgg gaggccggtc
    14101 ttgcccggct cgtgcagcag gcgctcgaag ccggtcagcc cgcccgcggc ggcgtggtcg
    14161 aaggccgtga gcccctgtct atcggcggcc gtgaggtcag ctcccgcatt gagcagggca
    14221 agtatgagcg gtcggcgatc ttcggcgacg gaataccacg tgggccaaga ggtctcgtac
    14281 atggcgggct tggcgtcacc cacctcgccg cagggcgggg cgctccagaa gtccccgagc
    14341 ggtgcatagc cgccaccggg cagcttgaaa tcataggggc cgacggcaag catcagaggc
    14401 gtgcgacctg gaatgttacg ggcgttgatg tccgcgccgc gcgagagcag gtagcgggcg
    14461 agttccgcct gcccgagagt ggcggccagg tgcagggcgg tgcgcccgcc ccggtccgtg
    14521 gcgttgacgt ccagaccgga gtcgagcagc gctttgagtt cgtccttgac gactgagtcg
    14581 ccgagggtct ttgagacgaa atccgcccag tcgtggttag cttcccaggt gcggcgagcg
    14641 ggactgggcg gcgcggcctt acccggcggc gcggcgtgcg gggcggcggg cgtccgcgca
    14701 ctgttgtcag cagcagcagg gggcgcgccc tctttcctga cccgcgccag catcccctgc
    14761 atctgcttca atgggaaggc gagcaggcgg gcggggatgg cgaagttgag gttctgccct
    14821 tcacggatgg aagagacggt gacgccgacc accctcccgt aggcgtccac aaccggcccg
    14881 ccggaactgc cgggcgagac cggagcgtcg atctggagga tgcctccctg cgaccgcacc
    14941 ccgctgagta ttcctctggt aaacgtgccc tccagcccct cggggttccc gacgacgtat
    15001 atgtcgtcgc cgacggagag ctgtcgcacg gtgtcgagtt ggagcggttg ggccgtcagc
    15061 ccttcggtct cgacgagcgc gacgtcgagg tcgggccagg cgacgatcca cttggtcgga
    15121 taggagcgtt ggccgccggc gagcttgacc gtgccgcgcc tggcctcgga acacgtcacg
    15181 acgtgcttat tggtgagtat ttgggtctgg gtgacgaaga agccgctgcc gtagcagccg
    15241 ggcttgccgc ccacaccctc catctcgata tagacgactg agggcagcac cttgcgtgcc
    15301 acctggccgg cgctgagagg ccgtcgcgta ggggtctgtg ccggccgccg acccggtgaa
    15361 ggcgtgtcgc catagccggg ctgaagagcg ctcagtgaga tgaagagtgc gaggcatatg
    15421 ttcgggagtg tagtcatagt ctcaggtcaa tcgcgggtgg cggaggacac ctctgctgct
    15481 agctcttgtc gccgcgatgg gtgaacctac gcaggtacgc ttgcccagcc tccgccgccg
    15541 atgtatctcc ttccttcttc gccgcaatct cgatgagagt cgcccccgtc cagtccaccg
    15601 cgaagcaaaa ttcaaactgc tcagtgttgt cccagaggat gccatcccga cgcacctccc
    15661 ggaagaactg agcgtcgtcc tcccccagcg cggagaagac cggctccagt atctcctctt
    15721 ctgtcctgac gccgaacacc atccctaact cgctgtccgg gtgatcgcac acccggtcta
    15781 tgatcaggta agtcatgaac tcatcgagcg cccgctcgtc acgtagcagc gcgcgcagca
    15841 atcgtctctg ccgctccaga tgctcatctg ccccctcctc gtcatctccg gctctttcaa
    15901 ccccgtcctt cctgacatct ccgaccagca cgcggaagtc cagcgtcatc cggtaatgct
    15961 tctctactat gaccgtatca ggctcgccgc gactcatcac cttctcctac ttttagcaac
    16021 gggatatgct cctgtccggc tgcgttcgtg gtcagcccct gcttattaga taagagtttg
    16081 ataagacgcc ctattggaca gcaggcgtcg tacgggttca gtttttagct gccctcgacc
    16141 agaccgacgc cgctgcacct ttgattactt ccatacttat cagtgaggcg tccccatatg
    16201 gactgcgttt cacctattcc actgttgaag gaacatgact aactccgggt ctgtttattt
    16261 ccccgggctt agatattgac gcccttgcga actatgaaag cggtgacggt tgtccctgtt
    16321 tctgctatct tctgtcgtcg gtatgtttga caactcggtg aaacggccac gcatgaagcg
    16381 gcttgttagt cctgaagatg ggttctgtta gggagaggtt tgaatatcaa ctcccggcag
    16441 ccgaagactt cacgacacgc ctaatagcaa agtgtattag cggtaataag ctaatacact
    16501 ttgctattag gcgcaagcgg gatttgggga ttagcgtcct gtcaggaggt actgcctact
    16561 gatgggaagc gcggcacgcc gtaagccagc ccgtctggcc gagaaactac tagccatcag
    16621 gagcagcctc ggcctttcac agaatgggat gatcaggcgg ataggatttg ttgatgagtt
    16681 aacccaagcc gagatcagca tgttcgagcg tggaatccgt gtgccgagcc tgcccgtctt
    16741 actcgaatat gcccgcgccg ctaatcttta cctggatgta ttagtcgacg attcattgga
    16801 cttgccacta agaattccag caccagccaa gcatgaaggc atcaagcgta aatccaatta
    16861 tagtaaaagg gctaagtaaa agtatcccgt catagacgca cttattaccg tactcccctt
    16921 tatcctttac cttctattct gatgcgtatg cggagtcgat ctgagggcaa agaagctaca
    16981 tgttcacggt cgcctgctta caacgtacat tgggaattcg taatccgtaa gacgacattt
    17041 gctattttgg tgccgtccct cagcgcccgt acgattgagg ggagattacg aatgaatgga
    17101 gagtgttatg ggagacgaaa gaagaagtga gatgacgcag ggcgaactcc tcagcgagat
    17161 gcaccggctt ggctacgacg atgtgaccga aagacagctt gccgactggc ggaggaaaga
    17221 cctgctcccg ccctttgacg taacgggtgg cggccggggg caacgacgcg ggagggagcg
    17281 cagctcatgg actgacggcg agtccgtcct caatcaagcg ctgtgggttc gcgaactcat
    17341 gcaaatctac aggagcacgg agagtatccg cctccccctc tgggtgctcg gctacccggt
    17401 tccactcaag cgcgtgcgcg aagcactcgg tgggccgcta aacgagatta ctaatgagat
    17461 tgctgaggcc attgagaatg aggcccgcgc gggcggcgag atcgaggaca tgattgagga
    17521 ggttacttac caccactttg aagagatgag acgcgaaggg gcagaggcgc tgctgctgcc
    17581 tcagcactcg ctggaggcgt tcttcaacgt cttctttaat caggactacg atttgaccga
    17641 cggggccttc gaactcggga tcgaggagtt gagggagtat gagactgcga tgcggcagag
    17701 gcaagacgcc gccctggcag ccgagggctt cagcgacact gagccggcgc gcgaggatga
    17761 cagcctgctc agattagtcg accgcgctcc gttcattaag cagtacttct cactccacca
    17821 gctcaagcaa gcggtcgatg aatgttcaga cagcgatctg cgggcggttc aacgtgacct
    17881 ttcctacttg cgcgggatga cccaactgct ccgcaagata atcacaattc tcacacgtga
    17941 gatacccccc gaatatcacc cgtccagggc agacagcttg cggcccatct tgagtgtagg
    18001 cggattaata atcttggcgg atttatccct gagacgtcat ggtttcgccc gggagattga
    18061 ctacttcctg ccggaagccc tgcggcgatt ccgacagcag tttactgaag agatggagcg
    18121 tgaactggtt gaggcgagta aacagatccc cgagatcatc gagacctgca ttcccatcat
    18181 agtgaacacc ttcttacagg aatcgaaggt agggtagcgg gaataagtga gagccgtgat
    18241 taacgactaa aaaggcatct ctatatttga actgtgaacg aagctccgag tcgtgacctc
    18301 atcttactgt tctttgacac ggcgatagcg tgatgcttca taaagaacat tatgatacac
    18361 gaattcccta tgcacgatct caggattatt gcagagcgcc tcgtgaagga gacgcgctag
    18421 aagaagaggc tgaggtttct taattccaca gaagagtagt tttgtggcat tagagcctag
    18481 gcgctcgtgt cgtcgtgcgc gagcgataag gacagttcgc ggtaggcgtc ggcctgggct
    18541 aacaggtctt tggccttctg ttcgactgcc tggacggcgt cggcgccggc ggcccagcgc
    18601 agcggaggct cgtcctgact cgcaagctgg atgagcgcct tggcgagctt ggcggggtca
    18661 ccgccttgca agccgttcat gcccttccag gcggtgacgg tctgcctggt tttctcggcg
    18721 tagtcgtcta tcgagggctg ggggtatttc gttgactcct ccgtgagcag ctcagtgcgg
    18781 aagaagcccg gctcgaccag catcgtgcgg atgccaaacg gcgcgacctc gggggtcagc
    18841 gactccatcc acccctcgac gccgaacttc gacgcgcagt aagcggaaac gaactcttgt
    18901 ccgatgatgc cggcaatcga agagatcatc acgacgagcc ccgagcgctg tgcgcgcatg
    18961 acaggcagcg cggcgcgggt gacgttcacc gggccgaata ggttggtctc gatctgtgcc
    19021 cggaagtctt cgggactgat ctcctcgaag aacccggcgt agaagttgcc ggcgttgttg
    19081 accagcacat ctatgcggcc aaaccgatca acagcagcct taaccgcggc ctcagcgtcg
    19141 gcagggttcg tcacgtccag cttcacaacc aacaggtccg cggattctcc gacggcctgg
    19201 gtcaccttgt cggtgttgcg acccgtagcc actaccttgt agccagcagc cagcgcagcc
    19261 ttagtaatat caacacccat accgcgcccc gcgcctgtaa taaaccaaac tttgctcata
    19321 tttgtacccc tttccttgca accttatcag atttcaactc aacaagcgct accggcaaca
    19381 aatcccaact gtaaagtcca atgtgttatt ccacaaaaag gttctttcgt agaattcgcc
    19441 ttaaagcaga ttcaacagaa gggttatttc gtggaatagc taaaagcaag ttggattact
    19501 cacgcgcctt taccagaaca accgttatgt catccgtggc gggaaaaccg gtcgcaaatg
    19561 tattgacatc atctaaaaga ctcttaacgg aagccgaagg gtttgaaacg tgatcatgca
    19621 agcgcaccgc tccgtattca tcgaacgatg aattgttggc ttcggtaatg ccatctgaat
    19681 ataggaaaag acgacttccc gccggcatct gtatttcgtg ctcggaaaat tcacactcaa
    19741 acattcccaa tggtaaaccg gcatcagttt ccaaaaaggt cgcgcctttc gaatcaatca
    19801 gtagagggct gaggtgtccc gcgctggcaa aggtcatggt acctttgttc ggatcaagaa
    19861 tggcgtaaat cattgttaca aacttagccg tcggaaaatc tttcagcagc acgcg
//
LOCUS       DASWNS010000450         7388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_251145, whole genome shotgun sequence.
ACCESSION   DASWNS010000450 DASWNS010000000
VERSION     DASWNS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251145"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..570)
                     /locus_tag="VGB76_15510"
     CDS             complement(88..570)
                     /locus_tag="VGB76_15510"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEY0100352.1"
                     /translation="MTDTSIIMREEQYERIVSVLHKLRAESFSRVVFLVDKNGQPIAV
                     QGDVSNIDTTSLASLAAGNVAATGGMAQLIGEKEFPTLSHEGERESIHICIIGRMLLV
                     VVFDDRSSLGLVKLRVKQASRELALIFEDVERDSLAKRQQDDALFAGITDDDIDSLFN
                     "
     gene            926..1909
                     /locus_tag="VGB76_15515"
     CDS             926..1909
                     /locus_tag="VGB76_15515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100353.1"
                     /translation="MSESVSKSAKRPASRPVTRSVSGGAPSKNRKLGAKTTARPKPLA
                     KRSGAEKAPVLKATKNKTASTSSAKSAKKSSRATASPAKPKAVSAKPQAANKNKTANT
                     TAGKTAASKTPARPAKSLAAAGKQSTHSAPATSGVAAKKQAARPKPLSAAARPHAPAP
                     PRQPTLDEAAALRAFERAHKEFARGRFAEARTQFRTLIEQHSGVAEVTARARTYLAIA
                     EARMRSESAIPRNAEELYDRGVIELNRGDFVAAQEMFERALKRDPDAAHIYYGLAATR
                     ARLGALEPALQSLGRALEIQPNLRVRAQHDPDLAPLRNEPEYERLIFASRP"
     gene            2072..3121
                     /gene="yvcK"
                     /locus_tag="VGB76_15520"
     CDS             2072..3121
                     /gene="yvcK"
                     /locus_tag="VGB76_15520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEY0100354.1"
                     /translation="MTGTERGLKLVAIGGGTGLSTVLAGLKRLVDRREGGGQWLDALA
                     AIVTVSDDGGSSGRLRDELQILPPGDIRNCMVALSEDSTLMGRLFRYRFRGEGHLGGH
                     SFGNLFLAALTDVTGDFTEAVRLSSEVLASKGRIYPATLNDVRLVAELEDGREVFGET
                     NITASHGTIRHLRLEPEQCLPLPEALTALRAADIITVGPGSLYTSILPNLLVSRVAET
                     IGETDAVKIFICNLMTQPGETDGYTARRHLETVKRYAPAINFDYVIVNNRLISEEQAT
                     RYRAEGAQQIGLSDHLLEEEFSHEAEIVRADLLDEGEKVRHSPEKLSRVIIACYEQAR
                     AATNAASARSVHSLA"
     gene            3137..4681
                     /gene="glmU"
                     /locus_tag="VGB76_15525"
     CDS             3137..4681
                     /gene="glmU"
                     /locus_tag="VGB76_15525"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY0100355.1"
                     /translation="MDTQQPPQTPQGTQTPATTAGASTNVASSPPNAPLDVLILAAGL
                     GTRMHSRTAKVLHRLGGRPLISHVCHTAAALVKEGRPLHIVVGHQAEEVKTAVAAELG
                     ASGANFIMQTEQRGTGHAVMTARDAFADANSTLLVLSGDVPLINHETLGALIHQHRTH
                     RGRGAACTLLTVKLEDPTGYGRIVRDSDGRFERIVEQKDATPEERQIKEINAGIYCFE
                     TRGLFGALARVQPANAQGEYYLTDAPGILRADGADVSIFTHTDAREVSGINTRVELAE
                     FERLLRLRTLRRLMLDSGVTIIDPAHTYVSPEAQIGHDTIIYPGVHIEGRTHIGAACE
                     IRSGTRISNSRIGSGVHIKDHTVIVDSDVADNCSVGPFAHLRMGAQLEEQSAVGNFVE
                     LKKSRLGRRAKAMHLTYLGDATIGDRTNIGAGTVTCNYDGVNKHPTVIEQDVKIGSDT
                     MLVAPVTVGARSVTGAGSVVTRDVPPDTLVAGVPATVKKDLRAAVPPADTPEQTEEQN
                     AVTSNK"
     gene            4771..6630
                     /gene="glmS"
                     /locus_tag="VGB76_15530"
     CDS             4771..6630
                     /gene="glmS"
                     /locus_tag="VGB76_15530"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY0100356.1"
                     /translation="MCGIVGYVGNKQVVPVIIEGLRKLEYRGYDSAGIAVVDEAHTLD
                     IRRAEGKLRNLEEAIRLKPLDGTYGIGHTRWATHGRPTEENAHPHRDCTGRVVVVHNG
                     IIENYLQLKERLRKTDHNFVTETDTEVIAHLIEEYLRQEERLEAAVQRAVRDLRGIFA
                     LSIISVDEPDLIIAVRQGPPVVVGLGDGEYFVASDIPPILQHTRDVFYLGEREIAVLT
                     RDSVRVTDFEGDLREPQQQRITWDPIMAEKGGFKHFMLKEIYEQPRAVRDTVQGRISL
                     DTGRVYLDEMNITEAGFKSFDRVMIVACGTSWHAGLAGKYMLEQFARIPVEVDYASEF
                     RYRDPVINERTLLVVISQSGETADTIAALREAKERGAKILSICNVQGSMITRESDGTI
                     LTHAGPEIGVASTKAFTAQMIALYLFGLYLGQVRGALSADEAKHHAQELAELPVKIEH
                     LLSESDSLEELARELHRSTDFLYLGRGINFPVALEGALKLKEISYIHAEGYPAGEMKH
                     GPNALIDERLPVVVINTYEEGNRASELRYEKTHSNIVEVKAREGRVVSVLTEGDEMSA
                     DASDHVIEIPASSDLLSPILAVIPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
     gene            6659..7015
                     /locus_tag="VGB76_15535"
     CDS             6659..7015
                     /locus_tag="VGB76_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100357.1"
                     /translation="MTNEEMERAIEFLLKNQADFEARMTRLEAIQAATSQQIRESAAE
                     TNQQISETNQQIAETNQQIRELSVRQDRTQVQLDHLSSVVATLAEATQRNSADIDVLV
                     KLVGSVIEGRNGKTGS"
     gene            complement(7197..>7388)
                     /locus_tag="VGB76_15540"
     CDS             complement(7197..>7388)
                     /locus_tag="VGB76_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100358.1"
                     /translation="LTWTQAQLDSIREVIRNYRIHLYPSTLLIDPEGKIVSLNQTRKG
                     QPSLRGQELIRSLDRLLPP"
BASE COUNT         1557 a         2318 c         2161 g         1352 t
ORIGIN      
        1 catagctttt aagtgacagg tgacgggtga cgggtgacag gagagaacaa ggattttatc
       61 ttgtcacccg tcaccagtca cctgtcacta attgaataag ctgtcaatgt cgtcgtccgt
      121 gataccggcg aagagcgcat cgtcctgttg ccgctttgcc agcgaatcgc gttcgacatc
      181 ctcaaaaatt aaggccagtt cgcgcgatgc ctgcttcaca cgcagcttga ccagaccgag
      241 actggaacgg tcgtcgaaga cgacgacgag gagcatccgc ccgatgatgc agatatgaat
      301 gctttcccgc tcgccttcat gcgaaagcgt gggaaactct ttctcgccaa taagttgcgc
      361 catgccgccc gtcgccgcga cgtttccggc ggcgagcgat gcaaggctcg tcgtgtcaat
      421 attgctcacg tctccctgca cggctatcgg ctggccgttt ttgtccacaa ggaagactac
      481 gcgagagaag gattcagcac gcagtttgtg taaaacagac acaatccgct catactgttc
      541 ttcgcgcata atgatggaag tgtctgtcat tagtacaggc gctttctcta ttggggactg
      601 actttggtgg gatggtggga ggccgctatc gagagccgcg tctaaaactt aaaccaagca
      661 tttataagga gttcgagcgt cggccagaaa caattgcccc atccctgaag ggtcgcacga
      721 ggggcgcaac tccttttgga ctctaacata acgacttcga taacatcaac tgtttttctt
      781 tgtttgtcgc tgctcgccag cccgctcaaa acccgcccag tacccgcaac ttacactccc
      841 acgttgattt atgcggcgcg ctatgttaaa ctccgcgtgt ttactggctc cgattaggga
      901 gtcattttga taatgggagc ttcgcatgag cgaatctgtc agcaagagcg cgaaaagacc
      961 tgcctctcgt ccggtcacgc gtagcgtctc tggtggcgca ccgtcgaaaa atcgcaaatt
     1021 gggcgcgaag accacggcgc gaccgaaacc gctcgccaaa cgctctggcg cggagaaagc
     1081 ccctgtcctc aaagcgacca agaataagac ggcctctacc tcctccgcca agtccgccaa
     1141 aaagagttcc cgcgcgaccg cttcgcctgc gaagcccaaa gccgtgagcg cgaagccgca
     1201 ggcggccaac aagaacaaaa ctgcgaatac taccgctggt aaaaccgctg ccagtaagac
     1261 ccctgcgcgt ccggcgaagt ctttagctgc cgccgggaag caatccacac acagcgcgcc
     1321 cgcgacgagc ggcgtcgctg ctaagaagca ggcggcgcga ccgaagcctc tgtctgccgc
     1381 cgcacgcccg cacgcgcctg cgccgccgcg ccagccgacg ctggacgagg ccgccgcgct
     1441 gcgtgccttc gagcgtgcgc ataaagagtt tgcgcgtggt cgttttgcgg aagcacgtac
     1501 gcagtttcgc acgctcatcg agcagcattc gggcgtggcc gaagtgacgg cgcgtgcgcg
     1561 cacttatctc gccatcgccg aggcgcgcat gcgttcggag agcgccatac cgcgcaacgc
     1621 cgaggaactt tatgatcgcg gcgtcatcga actcaatcga ggcgattttg tcgcagcgca
     1681 ggagatgttc gaacgcgcgc tcaagcgcga ccccgacgcg gcgcacatct actacggctt
     1741 ggcggccacg cgtgcgcgtc tcggtgcgct cgaaccagcg ctgcaatctc tggggcgcgc
     1801 ccttgagatt cagcccaatc tgcgcgtccg tgcgcagcac gacccagacc tcgcccccct
     1861 gcgcaacgag ccggaatacg aacggctcat cttcgcatcc cgtccctgat tctctttgtc
     1921 aactctcctc actattcgtc tgcacgattg acactaaagt tccattcttg acgcgccgct
     1981 ttgaaagcct ccggcttttg agtatgattt cagagcgcgc ggtcaatttg ccgtcgcgcg
     2041 agcgtctggg gagagcgaga agaatttgca tgtgacagga acagagcgcg gtctgaaact
     2101 ggttgccatc ggcggcggca cggggctttc caccgtgctg gccggactca agcgactcgt
     2161 ggacaggcgc gaaggcggcg ggcaatggct cgacgcgctc gcggccattg tcaccgtctc
     2221 ggacgacggt ggcagttcgg ggcgtctgcg cgacgaactc caaattctcc cgccgggcga
     2281 catacgcaac tgcatggtcg cgctctcgga agattcgacg ctcatgggtc ggctcttccg
     2341 ttatcgtttt cgcggcgaag gccatctcgg cggccacagc ttcggcaatc tctttctcgc
     2401 cgcgctgacg gacgtgaccg gagatttcac ggaagccgtc cgcctctctt ccgaagtgct
     2461 cgccagcaag gggcgaattt atcccgcgac gctgaacgac gtgcgcctcg tcgccgaact
     2521 cgaagacggc agggaagttt tcggtgaaac caacatcacc gcctcgcacg gcacgattcg
     2581 acatctgcgc ctcgaaccgg aacagtgtct ccctctgccc gaagcgctca ccgccctgcg
     2641 cgccgcagac atcatcaccg tcggtcccgg ttcgctctac accagcatcc ttccgaatct
     2701 gctcgtctcg cgcgttgccg aaaccatcgg cgagactgac gccgtcaaaa ttttcatctg
     2761 caacctgatg acacagccgg gcgagactga cggctacact gcgcgtcgtc atttagagac
     2821 ggtgaagcgt tacgcgcccg ccatcaattt cgattacgtc atcgtcaaca atcgtctcat
     2881 cagcgaggaa caggccacac gttaccgtgc ggaaggcgcg cagcagatcg gcctgtccga
     2941 tcatctcctg gaagaagagt tcagccatga ggcagagatc gtgcgcgccg acctgctcga
     3001 tgaaggagag aaggtgcggc atagtcccga aaagttgtcg cgtgtcatca tcgcttgcta
     3061 cgagcaggca cgcgcggcca caaacgccgc ctctgcgcgt tcggtgcaca gtctcgcctg
     3121 aacgcttgag aacataatgg acacacagca gcctccacag accccgcagg gaacacagac
     3181 gcccgcgacg accgcaggcg caagcacgaa tgtcgcgtcg tcgccgccga acgcgccgct
     3241 cgacgtgctc atcctcgccg ccggactcgg cacgcgtatg cactcgcgca ccgccaaagt
     3301 cctgcaccgg ctcggaggcc gcccgctcat ctcgcacgtc tgccacaccg ccgccgcact
     3361 cgtcaaagaa ggccgcccgc tccacatcgt cgtcgggcat caggcggaag aagtgaaaac
     3421 ggcggtcgcg gcggaactcg gcgcgagcgg cgcaaacttc atcatgcaga ccgagcagcg
     3481 cggcacgggc cacgccgtga tgaccgcgcg cgatgcattc gccgacgcaa attccacgct
     3541 cctcgtgctc tccggcgacg tgccgctcat caatcacgaa acgctcggcg cgctcatcca
     3601 ccagcatcgc acgcatcgcg ggcgcggcgc ggcctgcacg ctcctgaccg tcaagctcga
     3661 agacccgacc ggctacggtc gcatcgtgcg cgactcggac ggtcgcttcg agcgcatcgt
     3721 cgaacagaaa gacgccacgc ccgaagagcg gcagatcaaa gagatcaacg ccggaatcta
     3781 ttgcttcgaa acgcgcggcc tcttcggcgc gctcgcacgc gtgcagcccg ccaacgcgca
     3841 gggcgaatac tacctgacgg acgcgcccgg catcctgcgc gccgacggcg cagacgtaag
     3901 catcttcacg cacacggacg cgcgcgaagt ctccggcatc aacacgcgcg tcgaactcgc
     3961 agagtttgaa cgtctgctca gattgcgcac gctgcgccgt ctcatgctcg acagcggcgt
     4021 cacgatcatc gatccggcgc atacctacgt ctcgcccgaa gcgcaaatcg gccacgacac
     4081 gatcatctat cccggcgtcc atatcgaagg gcgcacgcac ataggcgcgg cctgcgagat
     4141 acgttccggc acgcgcatct caaactcgcg catcggcagc ggcgtgcata ttaaagacca
     4201 cacggtcatc gttgattccg acgtggccga caactgctcc gtcggaccct tcgcgcacct
     4261 gcgcatgggt gcgcaactcg aagagcaatc ggcggtcggc aacttcgtcg agctgaaaaa
     4321 atcgcggctc ggacggcgcg ccaaggcgat gcacctcact tatctcggcg acgcgacgat
     4381 tggagatcgc accaacatcg gcgcgggcac cgtcacctgc aactacgacg gcgtgaacaa
     4441 acatccgacc gtcatcgaac aggatgtcaa gatcggctcg gacacgatgc tcgtcgcgcc
     4501 cgtcacggtc ggcgcacgct ccgtcaccgg cgcaggctcc gtcgtcacgc gcgatgttcc
     4561 gcccgacacg ctcgtcgcgg gcgtcccggc tacggtcaaa aaagacctcc gcgccgccgt
     4621 gcctccggcg gatacacccg aacagacaga agagcaaaat gcagtgacaa gtaacaagtg
     4681 acaggtgacg agttaagaca aaattcgtgt ctttaacttg tcacctgtca cctgtcgctc
     4741 gtcgctgctt ttctgaaagg aaagcaagat atgtgtggga tcgttggata cgtcggtaac
     4801 aaacaggtcg tgcccgtcat catcgagggt ttgcgcaaac tcgaatatcg cggatacgac
     4861 tccgcaggca tcgccgtcgt tgacgaagcg cacacgctcg acattcgccg cgccgaaggg
     4921 aaactacgca acctcgaaga agcgatccgc ctcaaaccgc tcgacggcac ctacggcatc
     4981 ggccacacgc gctgggcgac gcacggcagg ccgaccgaag agaacgcgca cccgcatcgt
     5041 gattgcaccg ggcgcgtcgt cgtcgtgcat aacggcatca tcgaaaatta tctccaactc
     5101 aaagaacgcc tgcgcaagac agaccataac ttcgtcacgg aaaccgacac ggaagtcatc
     5161 gctcatctga tcgaggaata cctgcgccag gaagagcgtc tggaagcggc ggtgcagcgc
     5221 gccgtgcgcg acctgcgcgg catcttcgcg ctctcgatca tctctgtgga cgagccggat
     5281 ttgatcatcg ccgtgcgtca aggccctccc gtcgtcgtcg gactcggcga cggcgagtat
     5341 ttcgtcgcct cggacatccc gcccatcctg caacacacgc gcgacgtgtt ctatctgggc
     5401 gagcgcgaga tcgcagtcct cacgcgcgac tcggtgcgcg tcacggactt cgagggcgac
     5461 ctgcgcgagc cgcagcagca acgcatcacc tgggacccga tcatggcgga gaagggcggc
     5521 ttcaagcact tcatgctcaa ggagatttac gagcagccgc gcgccgtccg cgacaccgtg
     5581 caggggcgca tctctctgga cacggggcgc gtctatctcg acgagatgaa catcacggag
     5641 gccgggttta aaagtttcga ccgtgtgatg atcgtcgcct gcggcacctc ctggcacgcg
     5701 gggctggcgg gcaagtacat gctcgaacag ttcgcgcgca tccctgtgga ggttgattac
     5761 gcttcggagt ttcgttatcg cgatccggtc atcaacgaac ggacgctctt agtcgtcatc
     5821 tcgcaatcgg gcgagacggc agacaccatc gccgccctgc gtgaagcgaa agagcgcggc
     5881 gcgaagattc tctcgatctg caacgtgcag ggttccatga tcacgcgcga atccgacggc
     5941 acgatcctga cacacgccgg gccggagatc ggcgtcgctt cgacgaaggc tttcaccgcg
     6001 cagatgatcg cgctctatct cttcgggttg tatctggggc aggtgcgcgg cgcgctctct
     6061 gcggacgaag ccaaacatca cgcgcaggag ttggccgaac tcccggtcaa gatcgaacac
     6121 ctcctcagcg aatccgactc gctcgaagaa ctcgcacgcg agttgcaccg ctcgaccgat
     6181 tttctgtatc tcggtcgcgg catcaatttc ccggtcgcgc tcgaaggcgc gctcaagctc
     6241 aaagagatct cttacattca cgccgaaggc tacccggcgg gcgagatgaa acacgggccg
     6301 aacgcgctga tagacgaacg cctcccggtc gtcgtcatca acacgtatga agagggcaac
     6361 cgcgcctccg agttgcgtta tgagaagacg cattcgaaca tcgtcgaagt caaagcgcgc
     6421 gaggggcggg tcgtctcggt gctgacggaa ggcgatgaga tgtctgcgga cgcgtccgac
     6481 cacgttatcg aaattcccgc gtcgagcgat ctgctctcgc ctatcctcgc cgtcatccca
     6541 ctccaacttc tggcctatca catcgccgtc cgcagaggat gcgacgttga ccagccgcgc
     6601 aatctcgcca agtccgtgac ggtagagtga aatgcgcgac cgttaaggag aactcagtat
     6661 gactaacgaa gagatggaaa gggcgattga gtttttgttg aagaaccaag ctgattttga
     6721 ggcgcgcatg acgcgtctgg aagcgattca agcggcgaca agccagcaaa ttcgggaatc
     6781 ggcggcagag acaaatcagc agatctcgga gacaaaccag cagatcgcag agacaaatca
     6841 gcagatccgt gaattgtctg tgcggcagga tcggacgcag gtgcaactcg atcatttgag
     6901 cagcgtcgtg gcgaccctcg ccgaagccac acagcgcaac tctgcggaca tagatgtgct
     6961 ggtcaaactc gtcgggagtg tgattgaagg gcggaacggg aagacgggaa gttaaagtct
     7021 cgcgcgtcgt tctgtccggg ctgatagtgc gctgactata gagaggcgac ggagtgggca
     7081 tctccgtcgc ctctctgttt tcatgttcga cgtgagcaca ggttggtgaa actgtgtgtg
     7141 ggtttctgtg cgacctccgc gtcggtcttt gtcgtgccgc ttgagggtgg tttgatttaa
     7201 ggtgggagca ggcggtcgag cgagcggatt aactcctgcc cgcgtagcga gggttgcccc
     7261 tttcgagttt gattgaggga gacgatcttg ccttcggggt cgatgagcag ggtcgaaggg
     7321 tagaggtgga tgcggtagtt gcggatcact tcgcggatgc tgtcgagttg cgcctgcgtc
     7381 caggtcag
//
LOCUS       DASWNS010000451        13722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_354639, whole genome shotgun sequence.
ACCESSION   DASWNS010000451 DASWNS010000000
VERSION     DASWNS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13722
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354639"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..654)
                     /gene="recR"
                     /locus_tag="VGB76_15545"
     CDS             complement(40..654)
                     /gene="recR"
                     /locus_tag="VGB76_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY0100359.1"
                     /translation="MLDYAEPVTKLIDEFKRLPGVGQKSAQRLAFHILRMSEPDVARF
                     VAAMEEVKRKIVFCTVCNNLTDVDPCRYCSSTARDRSIICVVEEPYNLVAVEKTRSYH
                     GLYHVLHGSLSPIRGMSPDDLRLQNLLPRLRPENNDGVEVREVILATNPNTEGEATAS
                     YISRLLKPLGVRVTRIAMGMPVGSDLEYVDEVTMDKAMTNRHEM"
     gene            complement(758..1063)
                     /locus_tag="VGB76_15550"
     CDS             complement(758..1063)
                     /locus_tag="VGB76_15550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY0100360.1"
                     /translation="MKFPGGMNMQQMMKQAQQMQEKMQREMEALKVDATAGGGVVTVQ
                     MSGSKEVTSIKIDPEAVKEGDVEMLQDLIVAAVNEAGRKVDEAMQGKLGGMLPPGLL"
     gene            complement(1285..3174)
                     /locus_tag="VGB76_15555"
     CDS             complement(1285..3174)
                     /locus_tag="VGB76_15555"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY0100361.1"
                     /translation="MKHTPRRRLPVVTFICALALVFTYASNAQAQSCAPPPNGQVHWY
                     PFDGNTLDLAENQSGILRNGATFAPGKVGQALSLDGVDDYFEVNGFFLSMSFSALSME
                     TWINPTQVATGRIFDKAPPGGTNGFLLELSGGRLRAVFGANSATSAASIPAGAYTHVA
                     ATYDGTNIRLYINGALDSTTAATTPGAIASNSLNAFIGAGREAGTAFGGQIDEFAIYR
                     RTLTAGEIQAIYAAGGAGKCKAGRALISEFRFIGPTLSNDEFIEIYNNSDSTLVVQAT
                     DASGGWGVYDQGVGGGPRLLFVIPNGTQIPARGHYLAVNNGGAYSLANYGGTNAAAPD
                     ITYAVNLDSFDAITLASSANGDVTPDTRLDRVGFSGSSQFYLEGTGIPPGPAALNATS
                     QYSYIRRLASGVPQDTNDNAADFLLVSTEPATTGGVLGAPGPENLASPVQRNATIKPS
                     LVDPGCSGFGAPTSACARVRTAEGANPTNAAFGQLRLRRKFTNTTGAGVTRLRFRITD
                     ITTTPEAGVADLRVVGGSGNFTATLSGGGTTVIERLTLEEPPAQPNGGGFNSTVAAGT
                     IALGSPLLPNNSINVEFILGVQQEGSFRFFVNVEALPAPANPAPPAQSGAKASGMKAT
                     GRKQK"
     gene            complement(3350..3688)
                     /locus_tag="VGB76_15560"
     CDS             complement(3350..3688)
                     /locus_tag="VGB76_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100362.1"
                     /translation="MLKLKWPRRTKTGETEAGAPGDATAAAGVDEATITGGATRALDE
                     VRLRNSLEVIKTAVVWLVVINILTVLSPLLWLSLKWTLVVGVLLTAGSLSVGCVAYLR
                     ALRTQRQLPE"
     gene            3802..5886
                     /locus_tag="VGB76_15565"
     CDS             3802..5886
                     /locus_tag="VGB76_15565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY0100363.1"
                     /translation="MKRYVIKRPAGAAVPERLARYREELNEEQFAVATAPAGATLVIA
                     GAGSGKTRAITYRVAYLIEQGVTPSRIMLATFTNRAAREMLRRVEELTGGNNNAWRRV
                     WGGTFHRIANMVLRRHAAALDYTENYTILDAEDARDFLNVCVDEAGIDTKARRFPKAE
                     VLQDIISFANNTDRPIPDVVARNYPHFEPLTQAIMRVDSIFMERKRARNSMDYDDLLL
                     NWKRLLLEKPEIARLYAEQFEHILVDEYQDTNKLQAEIVDLLAVQHKNVMVVGDDAQS
                     IFAWRGAEWSNLYEFPARYPQARQFRLENNYRSTPEILAVANVSIASNRKQFPKTLRA
                     IRPSLGLSPALVPCHDADQQAAFVAARILELRDEGVPLKELAVLYRSHYHSLELQLEL
                     TRRGIPYQIRSGVRFFEQAHIKDVISYLRLVANPRDELAWKRVLRLIPNVGAATANRI
                     WEQIGYAPDPLALVLRGGADAAASRRGNSWAEFRALLDALVMNETRTNPAKQIELILA
                     RGYEAHLAANYENAETRLEDLRQLAHYSTRYNSTEEFLAELALLATERYGAPQPVSGE
                     EVVMGADEDELIALTSVHQAKGLEWRAVFIIWAADGKFPSPRSLRDAEGEEEERRLWY
                     VALTRARDQLYITYPLMITDYNRQTIVQRPSRFVTETPPELYEVWSLEEESAPLDALP
                     EIKESEPGGYLN"
     gene            5967..6701
                     /locus_tag="VGB76_15570"
     CDS             5967..6701
                     /locus_tag="VGB76_15570"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0100364.1"
                     /translation="MTNHNFNLPVERTLDARVKLYYDLHVPPQTPAPLLLALHGYGSN
                     KRWMMREARELAPEGFAVAAPQGFHQHLKVPKGADGKESKERGGALGFGFGWLTNFHP
                     EESVALHHRALLDILERLLEEGVADSSRIFLLGFSQTCALNYRFAFTHADLLRGVVGI
                     CGGLPGDWETSEQYRHTEAAVLHLAGTRDEFYPPARTADYAARLSLRARDVEFKSYDA
                     AHELDAAMRADVRAWLAAHTAGGSGE"
     gene            6756..7616
                     /locus_tag="VGB76_15575"
     CDS             6756..7616
                     /locus_tag="VGB76_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100365.1"
                     /translation="MSKANRGSKAKTGSRANGRQRAIELVRRRIVSGGRPRLLVSFLL
                     ALTGASGFLVSTALLHLGVTMMTVRYPLAVLSAYSVFLLLLRLWLAFQRKTHRLDDWV
                     PDLGDAAAQFLDVAARTPVNTADAATFGGGANFGGGGAGGAWTGGGGASSSSFDSGGS
                     SSIMDSVPDVGLDLDLDLGEGCFWIVIPIIAIAGVLVVMFYVVYVAPIFLAEILVDAL
                     LVAGLYKRLKGIEEPRHWLRSVVRRTLLPVMLATALFTAAGYLMGRIAPAAQSIGGFW
                     REINTPKDRL"
     gene            7696..8274
                     /locus_tag="VGB76_15580"
     CDS             7696..8274
                     /locus_tag="VGB76_15580"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0100366.1"
                     /translation="MDVERADFCEYSSRRTPIPSAKVKLPEMKFISRFAKARVRLASL
                     VLLVVAFVAAQTACGRSGESEQQAAAHASEIRAVLDAQAAAWNRGDVGGYMDGYERAE
                     TTTFISGDTVARGWQTVHDRYKKSYDTPAKMGTLAFSELEIKPLSEFYANVTGRWQLT
                     REKDTPHGRFALIFRRTGAGWRIVQDTTTSAS"
     gene            8375..10366
                     /locus_tag="VGB76_15585"
     CDS             8375..10366
                     /locus_tag="VGB76_15585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0100367.1"
                     /translation="MPRQLKFTHITNAVVFTFFFFCLALLPVAHAQTMSGLPDARTQA
                     SSSLRAPGAFPIERYLNIRSASSPTLDVRGERIAFLTNVTGTAQVWSIPSAGGWPEQL
                     TFYTDRVDFVRWSPDGTGLLFGKSRGGDENSQLFWMSPDGAQVRQLTDAPRVRYNFGG
                     WSADGRRISYASNKRNPNFFDIYTMEVATGVESLVYQQDGSNAVSAWSPDGRFIVVSR
                     ASDTLSLDNDLYLVEVASKGVTHLTPHDGATQYGDVHFSRDNRSLLLSTNARGEWLSL
                     ARMDLATRRIEIIDDTPWDIDATAISDDGQLFAYTLNRDGFSELYVRRLNAAGVPAKR
                     DAAIALPGKGVVGGLEFSGDARRLALVFNGARYNPDVWLYDLKTRKLRQVTRSSRASI
                     PQSSFVEPQLVHYKSFDGRDIPAWYYRPLSAGNTGTGGGRSQLAPLPVIVSVHGGPEG
                     QERPGFNPTYQYFLSRGYAVLAPNVRGSTGYGKTYTHLDDVKLREDSVKDLAAAVDWL
                     KTGGGADPRRIAVMGGSYGGYMTLAAITLYPDLWAAAIEMYGISNFETNLKNTSGYRR
                     KQREREYGTLAGDLEFLRSVSPIYKVNRIKAPLLVFQGKNDPRVPYTESEQIVKALRD
                     RNAPVEYTLFDDEGHGFVKLTNRLVVYPKIVDFLERHMK"
     gene            10484..11749
                     /gene="hutI"
                     /locus_tag="VGB76_15590"
     CDS             10484..11749
                     /gene="hutI"
                     /locus_tag="VGB76_15590"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY0100368.1"
                     /translation="MKPPMLAVLNCSQLVTLRGAPRPRVGAEMRELSIIEDGAMLVGV
                     DGRIARVGARGEVEPHITVECEVIDAGGRIVMPGFVDAHAHPVFAGTRAHEFEQRAEG
                     ATYQEIAAAGGGIRASVRATRAATEDELVAASKRYTGWFLRGGTTTVEAKSGYGLSAE
                     DELKMLRAVRRLNEETPLQYVPTFLGAHEVPDEYRSRRAAYVELIIREMLPRVEEEKL
                     AEFCDVFCEERVFTVDESRRILLAARSHQLGLRLHADQLSLSGGALLAAELGAATADH
                     LEHTDAAGIAALRAARVQPVLLPGSVYALGSRNYPAAREMIEAGLAVVLATDFNPGSS
                     PTPSISLVLSLASTQMRMTPAEGIVAATINAAYSLGRGRETGSLEAGKRADFCLHDCA
                     DYRELAYFFGLEHAYTVYVGGRLAFRRGA"
     gene            12173..12916
                     /locus_tag="VGB76_15595"
     CDS             12173..12916
                     /locus_tag="VGB76_15595"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0100369.1"
                     /translation="MIAESCIGLTENKLLALLPDEERTRLLPFLEFAVLAHGKVIFEP
                     DEPIQQVCFPLSGIISIVAPSESGASVEVVMVGAEGVTGIPALWGDGLSPNSRAVVQV
                     DGTGMLIRADALRREFYRGGTLQELLLRYTQTYLTQITQSVLCQCFHHIEARLARWLL
                     ECRLRTNSNELRLTQEYIAQMIGVRRAGVSGAVAQLKERGLIEHTRGTVWIINQAGLE
                     AASCECFQVIRAEVARLFGDAGQLVTNNA"
     gene            complement(13095..>13722)
                     /locus_tag="VGB76_15600"
     CDS             complement(13095..>13722)
                     /locus_tag="VGB76_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100370.1"
                     /translation="HTVFLNARAAEMSLPPAPLEIFYSTRGENYMGWFDQVSGLLQQY
                     QGGNTQQQQQSNNVDNDFDQFAQAAPQSALADGLAAAFRSNQTPPFGSMLGQLFGQSN
                     GTQRASILNTLISTLGPTVISQILVNRGASGLAGMLGGGQREVTPEMAEQVSPEVVQE
                     IATQAEQKDPSIIDMASNFYAEHPTLVKGLGAAALTIALAKVAANQQR"
BASE COUNT         2538 a         4308 c         4268 g         2608 t
ORIGIN      
        1 aaaaaccgtc actcgtcgct tgtcacccgt cactgctttc tacatttcgt ggcggttcgt
       61 catcgctttg tccatcgtca cctcgtcaac gtattcgagg tcgctgccga cgggcatgcc
      121 catggcgatg cgcgtgacgc ggacgccgag cggttttaag agacggctga tataactggc
      181 ggtggcttcg ccttcggtgt tcgggttggt ggcgaggatg acttcgcgca cttccacgcc
      241 gtcgttgttt tcggggcgca ggcgcggcag gagattttgc agacgcagat cgtcgggact
      301 catgccgcga atcggcgaga ggctgccgtg caggacgtga tagaggccgt gatagctgcg
      361 cgtcttctcc acggcgacga ggttgtaggg ttcttccacg acacagatga tcgagcggtc
      421 gcgcgcggtg gacgagcagt agcggcacgg gtcaacgtcg gtcagattgt tgcacacggt
      481 acagaagacg atcttccgct ttacctcttc catcgccgcc acgaagcgcg ccacgtccgg
      541 ctccgacatc cgcaaaatat gaaacgccag acgctgcgca gacttctgcc ccacacccgg
      601 caggcgtttg aattcgtcga tcaacttcgt aactggctcg gcgtaatcaa gcataaaaat
      661 agtgacgggt gacaagtgac gagtgacgag caggaaacaa gacaagttta aagacaggaa
      721 tgctgtttta acttgtcacc tgtcacttgt cactctctta gaggagtccg ggcgggagca
      781 tgccgccgag tttgccctgc atggcttcgt caactttgcg tccggcttcg ttgacggcgg
      841 cgacgatcaa gtcctgtagc atctcgacat cgccttcctt gacggcctcc gggtcgatct
      901 tgatggacgt gacttctttc gagccgctca tctgcaccgt taccacgccg ccgcccgccg
      961 tcgcgtcaac cttgagcgcc tccatctcgc gctgcatctt ctcctgcatc tgttgtgcct
     1021 gcttcatcat ctgctgcatg ttcatcccgc cgggaaactt catcgccgta cacctctctt
     1081 cgcgttaaaa ttagaaccag cctgcacgca ctcatcctaa catctccgct cgcctaatgt
     1141 ggaatggcga aagagccgga ggcgaaccta aaatggcgga ggccgccctg agccttcgct
     1201 ctcaagcggc ctccgctgtt tcattcgcag cgacggttcc cggcgtcgcc gtccgcgtga
     1261 gcgaactctt taattcgccc gtttctactt ctgcttccgg cctgtcgctt tcatgccgct
     1321 cgccttcgcg ccggactgag cgggcggagc cgggttggcg ggcgcaggca gggcttcgac
     1381 gttaacgaag aagcggaagc tcccctcctg ctgaacgccg aggatgaact cgacgttaat
     1441 cgagttgttc ggtagtaagg gcgaaccgag cgcgatggtg cccgccgcca cggtcgagtt
     1501 gaagccgccg ccgttcggct gcgcgggcgg ttcctccagc gtcaggcgtt cgatgacagt
     1561 cgtaccgccc ccgctcagag tggccgtgaa atttccgctg ccgccgacga cgcgcaggtc
     1621 tgccacgccc gcctccggtg tcgtcgtgat gtcggtgatg cggaagcgca ggcgtgtgac
     1681 gcccgccccc gtcgtgttgg tgaacttgcg tcgcaggcgc aactgaccga aagcggcgtt
     1741 cgtcggatta gccccctcgg cggtcctcac gcgcgcgcac gcgctggtgg gcgcgccgaa
     1801 gccggagcag ccgggatcga cgagtgaagg cttgatcgtg gcgttgcgct gaacgggcga
     1861 ggcgagattc tccggtccgg gcgcgccgag cacgccgccg gtcgtggccg gttcggtgga
     1921 gacgagcagg aagtcggctg cgttgtcgtt ggtatcctgc ggcacgccgc tcgccagtct
     1981 gcgaatgtag ctgtactggc tggtcgcgtt gagggcggcg gggccggggg gaatccccgt
     2041 gccttcgagg tagaactgcg aactgccgga gaagcccacg cggtcgaggc gggtgtcggg
     2101 cgtaacgtcg ccgttcgccg acgaggcgag ggtgatggcg tcgaaagaat cgaggttgac
     2161 cgcataagtt atatcgggcg cagcggcgtt tgtgccgcca tagttggcga gcgagtaggc
     2221 accgccgttg ttgacagcga gatagtggcc gcgcgccgga atctgcgtgc cgttcgggat
     2281 cacaaagagg agtcgcggcc ctccgccgac gccctgatca tagacgcccc aaccgccgct
     2341 cgcgtccgtc gcctgcacga cgagggtgga gtcggagttg ttgtaaatct ctatgaactc
     2401 gtcgttggag agggtcgggc cgatgaagcg gaactcgctg atgagcgcgc gcccggcctt
     2461 gcatttgccc gcgccgcccg ccgcgtagat ggcctgaatt tcgcccgccg tcagcgtgcg
     2521 ccggtagatc gcaaactcgt caatctggcc gccgaaggcc gtcccggcct cccgacccgc
     2581 gccgatgaag gcgttgagtg agttcgacgc aatcgcgccg ggcgtggtgg cggctgtcgt
     2641 gctgtcgagc gcgccgttga tgtagaggcg gatgtttgtg ccgtcataag tcgcggcgac
     2701 gtgcgtgtaa gcgcccgcag ggatcgaggc ggcgctggtg gcggagttgg ctccgaagac
     2761 ggcgcgcagg cgaccgcctg acagttcgag caggaacccg ttggttcctc ccggcggggc
     2821 tttatcgaag atacgtccgg ttgcaacctg cgtgggattg atccacgtct ccatgctcag
     2881 cgcgctgaaa ctcatggaga ggaagaaacc gttaacttcg aagtagtcgt ccacgccgtc
     2941 gaggctgagc gcctgcccga ccttaccggg agcgaacgtc gcgccgttcc tcaatatgcc
     3001 gctctggttc tccgcgaggt cgagggtgtt gccgtcgaac ggataccagt gtacctgacc
     3061 gttgggcggc ggcgcacaac tctgggcttg ggcgtttgac gcgtacgtaa agacgagcgc
     3121 gagcgcgcag ataaacgtaa cgacaggcag gcgacgacga ggcgtgtgtt tcatcggaaa
     3181 gtccttatct ttttggaagc ggcctaaagt taaccttgta cctgacagtg acgcggcggc
     3241 tcgcgtcctc gggaggagat gcggggcgga ttgtaacacg cggcggcaag cacctgacag
     3301 gcaaaagccc ctgcccggcg acctgcgccg caagcccccg cgctccgact tattcgggga
     3361 gctgccgctg cgtgcgtagc gcccgaaggt aagccacaca accgacggag aggctgcccg
     3421 ccgtcagcaa gacgccaacc accagcgtcc acttcagcga cagccagagc agcggcgaca
     3481 agacggtaag gatattgatg acgacgagcc agacaaccgc cgtcttgatc acttccagag
     3541 agtttctgag gcgaacttcg tccagcgccc tcgtcgcgcc gcccgttatc gtcgcctcat
     3601 cgacgcccgc cgccgccgtc gcgtcgcccg gcgcgcccgc ctccgtttcg cccgtcttcg
     3661 tcctgcgcgg ccatttcagt ttaagcatgt ctcattttat ataactctcg ccgtcgagcg
     3721 atatacaaaa atatcgcggg ctgtctgcgc ggcgttgatt cacagcccgc gtgctgtcgt
     3781 gctataaggc gagcctgact tatgaagcgt tatgtcatca aacgaccggc gggcgcggct
     3841 gtcccggaac gactcgcgcg ctaccgcgag gaactgaacg aggagcagtt tgcggtggcg
     3901 accgctccgg cgggtgcgac gctcgtgatc gcgggcgcgg gatcgggcaa gacgcgcgcc
     3961 atcacttatc gcgtcgccta tctcatcgag cagggcgtga cgccttcgcg catcatgctc
     4021 gccacgttca cgaatcgcgc ggcgcgcgag atgctcaggc gcgtcgaaga gttgacgggc
     4081 ggaaacaaca acgcttggcg gcgcgtctgg ggcggcacgt ttcatcgcat cgccaacatg
     4141 gtcttgcgtc gccacgccgc cgcgctcgac tatacggaga actacacgat cctcgacgcc
     4201 gaagacgcgc gcgattttct gaacgtgtgc gtggacgaag cgggcatcga cacgaaagcg
     4261 cgccgcttcc ccaaggccga agtcctgcaa gacatcatca gtttcgccaa caacacggat
     4321 cgtcccatcc cggacgtggt ggcgcgcaac tacccgcatt tcgaaccact cacgcaggcc
     4381 atcatgcgcg tggattcgat cttcatggag cgcaagcgcg cgcgcaactc gatggactac
     4441 gacgacctcc tgctcaactg gaagcgtctc ctgttggaga agccggagat cgcgcggctc
     4501 tacgccgaac agttcgagca tatcctcgtg gacgaatatc aggacaccaa taagttgcag
     4561 gcggagatcg tcgatctctt agccgtccaa cataagaacg tgatggtcgt cggcgacgac
     4621 gcgcagagca tcttcgcgtg gcgcggcgcg gagtggtcga acctctacga gtttcctgca
     4681 cgctacccgc aggcgcgcca gtttcgcctt gagaacaact atcgctcgac gcccgaaatc
     4741 ctcgccgtcg ccaacgtctc catcgcttcg aaccgcaaac aatttccgaa gacgctgcgc
     4801 gccatacgcc cctcgctcgg actctcgccc gcgctcgtcc cctgccacga cgcggatcaa
     4861 caggccgcct tcgtcgccgc gcgcatcctc gaactgcgcg acgagggcgt gccgctgaaa
     4921 gagttggccg tcctctatcg ttcgcactat cactcgctcg aactgcaact cgaactgaca
     4981 cggcgcggca tcccctatca aatccgcagc ggcgtgcgct tcttcgaaca ggcgcacatc
     5041 aaagacgtga tctcttacct gcggctcgtc gccaacccgc gcgacgaact agcctggaaa
     5101 cgcgtcttgc gtctcattcc gaacgtcggc gcggcgacgg ccaaccgcat ctgggagcag
     5161 atcggttacg cgcccgatcc gctcgcgctc gtgttgcgcg ggggcgcgga tgccgccgcg
     5221 tcgcgccggg gaaactcctg ggcggagttt cgcgcgctgc tcgacgcgct ggtgatgaat
     5281 gagacgcgca cgaacccggc caagcagatc gaactcatcc tcgcgcgcgg ctacgaagcg
     5341 cacctcgccg ccaactacga aaacgcagag acgcgactcg aagacttgcg ccaactcgca
     5401 cactacagca cgcgctacaa ctcgaccgaa gagtttctcg ccgaactcgc cctgctcgcc
     5461 accgaacgct acggcgcgcc gcaacccgtc agcggagaag aggtcgtgat gggcgcggac
     5521 gaagacgaac tgatcgcgct cacctccgtc catcaggcga agggcttgga gtggcgcgcg
     5581 gtcttcatca tctgggcggc tgacggcaag tttccctcgc cacgttcttt gcgcgatgcg
     5641 gaaggcgaag aggaagaacg acgcctctgg tatgtcgccc tcacgcgcgc acgcgatcag
     5701 ctctacatca cttacccgct gatgatcacg gactacaacc ggcagaccat cgtgcagcgt
     5761 ccctcccgct tcgtcaccga gacgccgccg gaactttacg aagtgtggag tctggaagag
     5821 gagagcgcgc cgctcgatgc gctgccggaa attaaggaga gcgagccggg cggttatttg
     5881 aattagcgcg cggggcgtcg ctgtctgacg ctcgaatcgt ggtagccggg cgcgctggtg
     5941 gcggaggcga aaaaagtttt tcagctatga ctaatcacaa cttcaacctg cccgtcgagc
     6001 gcacgctcga tgcacgcgtc aaactttatt acgacctgca cgtcccgccg cagacgcccg
     6061 cgcccctgct cctcgcgctg cacggctacg gcagcaacaa acgttggatg atgcgcgagg
     6121 cgcgcgaact tgcgcccgaa ggattcgccg tcgccgcgcc gcaagggttt catcaacacc
     6181 tgaaagtgcc gaagggggcg gacggcaaag agtctaagga gcggggcggc gcgctcggct
     6241 tcggcttcgg ctggctcacc aactttcacc cggaagagtc cgtcgcgctg catcatcgcg
     6301 cgctgctcga cattctggag cgtctgctcg aagaaggcgt ggccgacagc tcgcgcatct
     6361 tcctgctcgg cttttcgcag acgtgcgcgc tcaattatcg cttcgccttc acgcacgctg
     6421 atcttttgcg cggcgtcgtg ggcatctgcg gcgggctgcc gggcgattgg gaaacgtcgg
     6481 agcaataccg gcacacggag gccgccgtgc tgcatctggc gggcacgcgc gacgagtttt
     6541 acccgcccgc gcgcaccgca gactacgcgg cacgcctgtc tctgcgcgca cgcgatgtcg
     6601 agttcaaaag ttatgacgcg gcgcacgaac tcgacgccgc gatgcgcgca gacgtgcgcg
     6661 cgtggttggc cgcacacacg gcgggcggtt ccggtgaata ggaacttgaa atgagtagga
     6721 acttgaaatg agtagcggct ggcgggaaac ttctgatgag caaagcgaat cgcggaagta
     6781 aggcgaaaac aggatcgaga gcgaacgggc gtcagcgcgc gattgaactc gtgcggcggc
     6841 gaatcgtgag cggcggcagg ccgcgtctgc tcgtctcatt cctgctcgcc ctaacgggcg
     6901 cgtccgggtt tctcgtctcg accgcgttgc tgcatctcgg cgtcacgatg atgacggtgc
     6961 gctacccgct cgcagtgctc tcggcctact cggtgttcct ccttctgctg cgcctctggc
     7021 tcgccttcca gcgcaagacg caccgcctcg acgactgggt tccagacctc ggcgacgccg
     7081 ccgcgcagtt tttagatgtg gccgcgagaa ctccggttaa cactgccgac gcggccactt
     7141 tcggcggcgg ggcaaacttc ggcggcggcg gcgcgggcgg cgcttggacg ggcgggggcg
     7201 gcgcgtcgag cagcagtttc gacagcggcg gtagttcgtc catcatggac tccgtgccgg
     7261 atgtcgggtt agacctcgac ctcgatctgg gcgaggggtg cttctggatc gtgatcccga
     7321 tcatcgctat cgccggggtg ctcgtggtga tgttctacgt ggtctatgtc gcgcccatct
     7381 tcctcgcgga gattctggtt gacgcgctcc tcgtcgcggg cttgtacaaa cgcctgaaag
     7441 gcatcgagga gccgcgccac tggctgcgct cggtcgtgcg ccggacgctc ctgcccgtca
     7501 tgctggcgac cgcgctcttc accgccgccg gatacctgat ggggcgcatc gcccccgccg
     7561 cgcaatccat cggcggcttc tggcgcgaga tcaacacacc gaaagataga ctctgaaaca
     7621 gacggagcga ctcgcagccg gacaaacttc gagatcgact cgccgcaagg cgcgtccgaa
     7681 tccgctcgcc gcgagatgga cgttgaacga gccgacttct gcgagtatag ttcacggcgc
     7741 acgcccatcc cttccgcgaa agtgaaattg ccggagatga aattcatatc acgctttgca
     7801 aaggctcgcg ttcggctggc atcgcttgtt ctgctcgtcg tcgccttcgt cgcagcgcaa
     7861 acggcttgcg gtcggtcggg cgaatcggag cagcaggcgg cggcgcacgc gtcggagatt
     7921 cgcgccgtgc tcgacgcgca ggcggcggcc tggaatcgcg gcgatgtcgg cggctacatg
     7981 gacggctacg agcgcgccga gacgacgacc ttcatctccg gcgacaccgt ggcgcgcggc
     8041 tggcagaccg tccacgaccg ctacaaaaag agctacgaca cgcccgccaa gatgggcacg
     8101 ctcgccttca gcgaacttga gatcaaaccg ctcagcgagt tttacgccaa cgtcacgggg
     8161 cgctggcagt tgacgcgcga gaaggacacg ccgcacggtc gcttcgcgct catcttccgc
     8221 cgcaccggcg cgggctggcg catcgtgcag gacacgacga cctccgccag ttgagctttc
     8281 aggtcaatcg ttcgcgtcga gctttttagt cccctgccag ttgataactt tcaactctta
     8341 aggatggttc ataacaataa ggatggttca caacatgcca cgccagctca agtttacgca
     8401 cattaccaac gccgtcgtct ttaccttctt cttcttctgc ctcgcgctgc tgcccgtagc
     8461 gcacgcgcag acgatgagcg ggcttcccga cgcgcgcacg caggcgtcga gttctctgcg
     8521 cgcgcccgga gccttcccca tcgagcgtta tttgaacatc cgctcggcct cttcgcccac
     8581 gctcgacgtg cgcggcgagc gcatcgcgtt tttgacgaac gtgacgggca cggcgcaggt
     8641 ctggagcatc ccttcggcgg gcggctggcc ggaacagttg accttctaca cggatcgcgt
     8701 ggacttcgtg cgctggtcgc ccgacggcac gggtctgctc ttcggcaagt cacgcggcgg
     8761 cgacgagaac tcgcagctct tctggatgtc gcccgacggc gcgcaggtgc gccaactcac
     8821 agacgcgccc cgcgtgcgtt acaacttcgg cgggtggtcg gctgatgggc ggcgcatcag
     8881 ctacgcctcg aacaaacgta acccgaactt tttcgacatc tacacgatgg aagtggcgac
     8941 gggcgtggag tcgctcgtct atcaacagga cggctcgaac gccgtcagcg cgtggtcgcc
     9001 cgacgggcgt ttcatcgtcg tctcgcgtgc gagcgacacg ctcagtctcg ataacgacct
     9061 ctatctcgtc gaagtggcgt cgaagggcgt cacgcatctc acgccgcacg acggggcgac
     9121 gcaatacggc gatgtgcact tctcgcgcga caatcgctcg ctcctcctga gcacgaacgc
     9181 gcgcggcgaa tggctctcgc tcgcgcgcat ggatttggcg acgcgccgca tcgagatcat
     9241 agacgacacg ccctgggaca tagacgccac cgccatctcc gacgacggcc aactcttcgc
     9301 ctacacgctc aaccgcgacg gcttcagcga actctacgtg cgacgcctga acgctgcggg
     9361 cgtccccgcg aaaagagacg cggccatcgc gctgccgggc aaaggcgtgg tcggcgggct
     9421 ggagttttcc ggcgacgcaa gacggctcgc gctcgtcttc aacggcgcgc gttacaaccc
     9481 ggacgtgtgg ctctacgatc tcaagacgcg caaactccga caggtcacgc gctcctcgcg
     9541 cgccagtatt ccgcaaagct cctttgtcga accgcagctc gtccattaca agagtttcga
     9601 cgggcgcgac attcccgcct ggtattaccg gccactgtct gcgggcaaca ccggaacagg
     9661 tggcggcagg tcgcaactcg cgccgctgcc cgtgatcgtc agcgtgcacg gtggtcccga
     9721 aggacaggag cgtcccggtt tcaacccgac ttatcaatac ttcctctcgc gcggctacgc
     9781 cgtgctcgcg ccgaacgtgc gcggctcgac cggctacggc aagacctaca cgcacctcga
     9841 tgacgtgaaa ctgcgcgaag actccgtgaa ggacttggcc gccgccgtcg attggttgaa
     9901 gacgggtggc ggggcagacc cgcgccgcat cgccgtcatg ggcggctctt acggcggcta
     9961 catgacgctc gccgccatca cgctctatcc agacctctgg gcggcggcca tcgagatgta
    10021 tggcatctcg aacttcgaga cgaacctcaa gaacacctcc ggctatcgcc gcaagcaacg
    10081 cgaacgcgaa tacggcacgc tcgcgggcga tctggaattc ctgcgttccg tctcgcccat
    10141 ctacaaagtg aatcgcatca aagcgccgct cctcgtcttt cagggcaaga acgatccgcg
    10201 cgtcccttac acggagtcgg agcagatcgt gaaagccctg cgcgaccgca acgcgcccgt
    10261 cgaatacacg ctctttgacg acgaagggca cggcttcgtc aaactcacca accgcctcgt
    10321 cgtctatccc aagatcgtgg actttctgga acgacacatg aagtaagtta cgaatttcag
    10381 gactcagagt gcgtcgccaa actatcttct gacttccttt gcgcctttgc gagagatcaa
    10441 actcacgcaa aggcgcaaag ccgcaaaagg agagcttaac aacttgaaac ctccgatgct
    10501 cgccgtcctc aactgctcgc aactcgtcac gctccggggc gcgccgcgcc cgcgcgtggg
    10561 ggcggagatg cgcgagcttt cgatcattga agacggggcg atgctcgtcg gcgttgacgg
    10621 gcggatcgcg cgtgtgggcg cgcgcggcga ggtcgagccg catatcaccg tcgagtgcga
    10681 ggtgatagac gcgggtggcc gcatcgtgat gcccggcttc gtagacgcgc acgcgcatcc
    10741 ggtctttgcg ggaacgcgcg cgcatgagtt cgagcagcgt gcggagggcg cgacgtatca
    10801 ggagatcgca gcggcgggcg gcggcatccg tgccagcgtg cgcgcgacgc gggcggcgac
    10861 ggaagacgaa ttggtggcgg cgtcgaagcg ttacaccggg tggtttctgc gcggggggac
    10921 gacgaccgtg gaggccaagt cgggttatgg attgtctgcg gaagacgagt tgaagatgct
    10981 gcgcgccgtg cgccgtttga acgaggagac gccgctgcaa tacgtgccga cattcttggg
    11041 cgcgcacgaa gtgccggatg aatatcgctc ccggcgcgca gcctatgtcg agttgatcat
    11101 cagagagatg ttgccgcgcg tggaggaaga gaagctggcg gagttctgcg acgtgttttg
    11161 cgaagagcgc gtgttcacgg tggacgaatc gcgtcgcatc ctcctcgccg cgcgctcgca
    11221 tcaactcggt ctgcgcctgc acgcagatca actttcgctc tccggcggcg cgttgctcgc
    11281 cgccgaactc ggcgcggcga ccgccgacca tctcgaacac acggatgccg cgggcatcgc
    11341 cgcgctccgc gccgcgcgcg ttcagcccgt gctgctgccc ggttcggttt atgcgctcgg
    11401 ctcgcgcaac tatcctgcgg cgcgagagat gatcgaagcg ggactcgccg tggtcctggc
    11461 gaccgacttc aaccccggct cttcgcccac gccctccatc tctctggtac tctcgctcgc
    11521 ctccacgcag atgcgcatga cgcccgccga agggattgtc gcggcgacga tcaacgccgc
    11581 ttacagtctc gggcgcgggc gcgagacggg gtctttagag gcgggcaagc gcgccgattt
    11641 ctgtctccac gactgcgcgg attatcgtga actcgcatac ttcttcggcc ttgaacatgc
    11701 gtacacggtc tacgtcgggg ggcgtctcgc gttccggcgc ggggcttgaa gttttgccgc
    11761 aagcgagcga ccaaaaataa ttgttgacgc aaaacttatt gattctgtat atttgcttca
    11821 acactggaaa ggaggtgatc aaaaaacgta tatgagttct tatcacagtg aggtggctgc
    11881 ggcctgacct ccggattgaa aacttgaatt ggtagggttt tcgtccgtcc gtcgctgaaa
    11941 caaaagcggc acacaggcca atcccaacag ttcaccggaa aaccgcctac gttaaatcgt
    12001 aggcggtttt tctcttttga ctcccggcaa acaaacccgc ctccgctcct cgcgccggag
    12061 gtgttgaccg aagccgcgat tcgttataaa aagtttagaa ttgcgggaaa aaattgcttg
    12121 aaggttaagt cagtttgctc cctgtatttt aaagatttga gaggtcttga atgtgatcgc
    12181 ggagagctgc atcgggctga ctgaaaataa actactcgct ttattgccag acgaagaacg
    12241 cacgcgctta ctgccatttc ttgaattcgc cgtgctcgcc cacggcaaag ttatcttcga
    12301 gccggacgaa cccatccaac aggtctgttt ccctttgagc ggcatcatct cgattgtcgc
    12361 cccgtcggag agcggagcga gcgtcgaggt ggtgatggtc ggagcggaag gcgtgacggg
    12421 catcccggct ctctggggag acggcctttc gccgaacagc cgcgccgtgg tgcaagttga
    12481 cggcacaggc atgttgattc gcgccgacgc gctccggcga gagttttaca gaggcgggac
    12541 gctccaggag ttgctgctgc gctatacgca gacttacctc acgcagatca cgcaatccgt
    12601 cctctgccag tgtttccatc atatcgaggc gaggctcgca cgctggctgc tggaatgccg
    12661 tctgcgcacg aactcgaacg aacttcgtct cacacaggaa tacatcgcgc agatgatcgg
    12721 cgtgcggcgc gcgggcgtga gcggcgcggt agcgcagtta aaagagcgcg gcttgatcga
    12781 gcacacgcgc ggcacggtct ggatcatcaa tcaggcgggg ctggaggcgg cgtcgtgcga
    12841 atgctttcaa gttatccgcg ccgaggttgc gcgcctcttc ggcgatgccg gccaactcgt
    12901 gacgaataac gcctgagcgt gagactaaag gccggctgca atcaagcgcg cctgaaaacc
    12961 ttcatcgcca cttaaagaca cgtaagaaca cgaagggggc gcggagagtg aatcaactct
    13021 ccgcgccccc ttcgtgttct tacgtgttga gtcttaatgc ttaaggctca agtagaaatt
    13081 tccgtccttc cttttcacct ctgctgattg gcggcgactt tggcgagggc gatggtgagc
    13141 gcagctgcgc ccagaccttt cacgagcgtc ggatgctctg cgtagaagtt gcttgccatg
    13201 tctatgatcg aagggtcttt ctgttccgcc tgcgtggcga tctcctgcac gacttcgggc
    13261 gacacctgct cggccatctc cggcgtcact tcgcgctgcc cgccgcccaa cattccggcc
    13321 agaccggacg cgccgcgatt caccagaatt tgcgagatca ccgtgggacc taaagttgag
    13381 atgagcgtgt tgaggatgct ggcgcgctgc gtgccgttcg attggccgaa gagttggccg
    13441 agcatgctgc cgaagggcgg cgtctggttt gaacgaaacg cggcggccaa gccgtcggcc
    13501 aacgccgatt gcggcgcggc ctgcgcgaat tgatcgaaat cgttgtccac gttattcgac
    13561 tgctgctgtt gctgcgtgtt gccgccttga tactgttgta agagtccgct gacctgatcg
    13621 aaccaaccca tataattttc acctctcgtc gagtaaaaga tttcaagcgg cgcgggcggc
    13681 agagacatct ctgccgcccg cgcgtttaga aaaaccgtgt gt
//
LOCUS       DASWNS010000452         5343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_17533, whole genome shotgun sequence.
ACCESSION   DASWNS010000452 DASWNS010000000
VERSION     DASWNS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17533"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VGB76_15605"
     CDS             <1..416
                     /locus_tag="VGB76_15605"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100371.1"
                     /translation="FRIGLAYEQLGKYEEAVNAFQSAIALSIDNSAAKATATFKGGSD
                     ARASMAHTYALMGKHDAARSVLAELEEHARHAYVPPHDMAMIYSGLRENDKAFEWLKK
                     AYQERYSLLVFLELDPRFDNLRVDPRFNDLLSQAT"
     gene            413..844
                     /locus_tag="VGB76_15610"
     CDS             413..844
                     /locus_tag="VGB76_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0100372.1"
                     /translation="MTSTQVYPVIYVLDGDFLFGMAAGLTRFTNWFRGVPEPIIVGIS
                     YGTESSDQWPQLRERDFKIPEVRDALGDSAANLFLDALTQEMIPFIEANYRTVPSDRC
                     LYGYSSSGFFVLYALFHQPDAFQRYISGSGDLYIAYPFGRP"
     gene            1043..1174
                     /locus_tag="VGB76_15615"
     CDS             1043..1174
                     /locus_tag="VGB76_15615"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0100373.1"
                     /translation="MEMSLVQQAIEQSGGNQTKAAELLGISRDQLRYRLKKIEEAAS"
     gene            1423..1590
                     /locus_tag="VGB76_15620"
     CDS             1423..1590
                     /locus_tag="VGB76_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100374.1"
                     /translation="MQLARDQAALSNAHRRNVSVEFDPPGEPFILPLDAAQVEDAVLN
                     LVINVVEAVEA"
     gene            complement(1692..2465)
                     /locus_tag="VGB76_15625"
     CDS             complement(1692..2465)
                     /locus_tag="VGB76_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100375.1"
                     /translation="MQTTARITIYLQALGGKFLGPNAYKTDSIKLSLKLPGNTLPITY
                     QLGDNVNDGAIGPNFLSSLPSIKGISSFLPILTPLPATGSDPAVNYLTPNNLTICGTV
                     AVTIPQPSVLGTVSVSIPRPQGDDLVLTQSIALNQMQSDYRVIVVVPGLLLEKPPASA
                     IPDMSGSIFVYVKMMCGCPITTGIPKSYWARDDFLVSAEVIYKNGGGDRQSLTFMSDA
                     TPSLFRAAVNEIANIKLVNFTARQKSTGNIGYLSVDYPV"
     gene            2980..3114
                     /locus_tag="VGB76_15630"
     CDS             2980..3114
                     /locus_tag="VGB76_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100376.1"
                     /translation="MSDTRSRHLPMMSSRREPETLMRYDHDRENLSQNLFNFPGYEEV
                     "
     gene            3314..4606
                     /locus_tag="VGB76_15635"
     CDS             3314..4606
                     /locus_tag="VGB76_15635"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0100377.1"
                     /translation="MSIDSSVIAAIEAAVKAEPENAELRLHLALLLLKAGRAQDALDH
                     CQKILAAQPDHAGALHCAANASESLGERQRGEGYRRIAAALGGTPDAKAAQGDESKPL
                     AEALEEIFGPDTGRRDDKGEGKAVPRRVVDGDTTAGHWEAEPSDIKLSDVAGMEEVKR
                     RLHLSLLAPLRNPEMMKLYGKSLRGGLLFYGPPGCGKTFIARAVAGEMGARFIAIGLA
                     DVLDMYIGQSERNLHEIFETARRTAPCVLFIDEIDALGRKRSLQRHSGGRDVVNQLLS
                     ELDSIGNDNRGVFVLAATNHPWDVDTALRRPGRFDRTLLVLPPDQTARAAILRYHLGE
                     RPVEGVDLRWVAEKTDGLSGADLAYVVESATELALEESISTGKPRPIRQNDFKRALKE
                     IKPSIRPWFEVARNYAMFSNEGGMYDDLLDYLRAKRML"
     gene            4756..>5343
                     /locus_tag="VGB76_15640"
     CDS             4756..>5343
                     /locus_tag="VGB76_15640"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100378.1"
                     /translation="MNEESFINHAGGHVERARILIEQDRYALAESELRHHILDEPEDA
                     TAHALLALTLSQQNKHREAVESARHGIHLAPMFPYVHYILAHVYNQQDQLDGAEKAIN
                     EAIRLDPEEADYFALLSNVKLRRRRWQEALEAAETGLYFNSEHVGCINLRAMALNQMG
                     MSDEAAVAIEGALSVEPENALTHANRGWQELHRGNY"
BASE COUNT         1141 a         1506 c         1587 g         1109 t
ORIGIN      
        1 actttcggat cgggttagcc tacgagcagc tgggaaagta tgaagaggcc gtcaatgcct
       61 tccagagcgc gattgctctt tcgatagaca attcggcagc gaaagcgacg gcgacgttca
      121 aaggcggttc ggatgcgcgt gcctcgatgg cgcatactta tgcactgatg ggaaaacacg
      181 acgcggcgcg aagcgtgctt gcggaactgg aagaacacgc cagacacgcc tacgtcccgc
      241 cccatgatat ggctatgatc tattcgggtc tgagagaaaa tgacaaggcc ttcgagtggc
      301 tcaagaaagc ttatcaggag cgttatagcc tgctcgtctt tctcgaactc gatcctagat
      361 tcgacaacct gcgcgtcgat ccgcgcttca acgatttatt gagccaggct acatgacatc
      421 cacgcaggtg tatcccgtga tttatgtttt agacggtgat ttcctctttg ggatggcagc
      481 cgggttgact cgcttcacca attggttcag gggagtccca gagcccatca ttgtgggcat
      541 tagctacggc acggaaagct ctgatcaatg gcctcagttg cgagaacgcg actttaagat
      601 tcccgaggtg cgcgacgcgc ttggcgatag tgcggcgaac ctctttttag atgccctgac
      661 gcaggagatg atccctttta ttgaagcgaa ctatcgtacc gtcccgtctg accgttgcct
      721 gtatggctat tcgtcgagcg ggttctttgt gctctacgcg ctgttccatc agccggatgc
      781 ctttcagcgg tatatctccg gcagcggcga tctgtatatc gcgtacccgt tcggtcggcc
      841 ataaaaggcc gtctggatga atcaaggtag ccgcgtgggt atcactcgcg cggctacctt
      901 gattggctct ggtgcaaata gcaatccagg atacgctgaa gcaacgcaag ctttaacttc
      961 aggcgattca cactaactcc cagggcaggc gggaaacaat cacaacgtcc gtcttccgcc
     1021 cggcgggctg tcgctcgata aagtggaaat gtcactggtg caacaggcca tcgagcaaag
     1081 cggcggcaac cagacgaagg ccgccgaact gctgggcatc tcccgcgacc agcttcgcta
     1141 tcgtctcaag aagatcgaag aggccgcgag ttgatcgtga gcattgattg gtgccgggca
     1201 tgagcggaag agcagacgag actttgccgg ggagcacgga gcacgggcag agcgcggagg
     1261 atgcgctcgc cgagtcgctc aaggaactgg cggatattaa gtttgcgctg gacgagtcct
     1321 cgatagtggc gattcacaga ccagcgtggc atcatcaatt acgtcaacga caagttctgc
     1381 gaagtcgccc ccgcctcgct tgcggaggtc gtccatcacg cagtgcaact cgcgcgcgat
     1441 caggctgcgc tctccaacgc ccaccgccgg aatgtcagcg tcgagtttga tccgcccggc
     1501 gaacctttca tccttccgct ggatgccgcg caggtcgagg acgcggtgtt gaatcttgtg
     1561 atcaacgtcg tcgaagccgt ggaagcgtaa tttacgcagt cgtcgctctt cgaggctgaa
     1621 atatggccgt caaccgcgcg gcatactcct tgcacttccg cactcacgta aaggagaagt
     1681 gcccgccact gtcaaacggg gtaatcgact gagagataac cgatattgcc tgtgctcttt
     1741 tgtcttgcag tgaaattcac cagcttgatg tttgcgatct catttacggc tgcacgaaaa
     1801 agcgatggcg ttgcatcgga cataaaggta aggctctgcc tgtcgccccc tccattttta
     1861 taaatgacct ctgcggacac gagaaagtca tctcgcgccc agtaggactt gggtatccct
     1921 gtcgtgatgg gacatccgca catcatctta acgtatacaa agatgctgcc ggacatgtcg
     1981 ggtatggcgg acgccggggg cttttccagc agaagccccg gcaccaccac gatcactctg
     2041 tagtctgatt gcatctgatt aagcgcaata ctttgtgtga ggacaaggtc atctccttgc
     2101 ggcctcggga tactcacgct cacggtaccc agtacgcttg gttggggaat ggtgacggcg
     2161 acagttccgc aaatcgtgag gttgtttggc gtcaggtaat tcactgccgg atcgctcccg
     2221 gtagcaggca gcggagtgag gatgggcaag aaagaagata tccccttaat cgaggggagg
     2281 ctgctcagga agttgggacc tatcgcccca tcgttaacgt tatcacctaa ctggtatgta
     2341 ataggaaggg tgttgcccgg cagttttaag gagagtttga tgctgtcggt tttgtaagca
     2401 ttaggtccca agaatttacc tcccaatgcc tggagataga tggtgattct ggcagtcgtt
     2461 tgcatggatt tggtttcctc ttccgggtag atttgccacc gaaattattt agatgccttt
     2521 gtagattaac ccgggatcga atgctagacc ccggagtgtc cgtgactatg tctttggctt
     2581 gagtatcttg tcaagactta tccgcgcttg atttcagccc gcatttcgcg aggacgtgga
     2641 caccgccttg tggcttaagg gagacgaaga cctcaagaac ctcgtcgcct gcggtcaagc
     2701 gactacgtgc ttcaatctcc tgcgctacct gcttgagaaa aggtctgcac aactcagcac
     2761 cgccggatca cgagagagcg gcctttatga ggatgccggt aagctgtggt cgcagctaaa
     2821 ggatgatcct taccgactct tacgttaata acccttcgcc tccatgcgag cctgcgaata
     2881 actttttttg cttcgggcgg cagcaaactt ttatcccttg atgagcggaa gccccttcgt
     2941 ctcctttagc gaagagtctg ctattgaagg tgacaagact tgtctgacac acgctcccgg
     3001 catttgccga tgatgtcgtc gcgccgcgag ccggaaacgt tgatgagata cgaccatgac
     3061 agggagaatc tgtcgcagaa cctcttcaac ttccccggtt atgaagaggt gtgaagcctc
     3121 tggtatcctc aggagcaggg aatggcgtgg ctcgtggaat acggagagtc cgttaattcg
     3181 ggcatcaaag tttcctgaga gattattttc gcaacacagc cttttttcgt catatagaac
     3241 ggtgcggagg aaggcgtaga gactttgaga gagagcgcgc gcagggattg agcgtgcgcg
     3301 tgataccgaa accatgagta tagattcgag cgttatcgcg gccatcgagg ccgccgtcaa
     3361 ggccgaaccg gagaacgcgg aactgcggct gcacctcgcc ctgctgctcc tgaaggcagg
     3421 gcgcgcacag gacgcgctcg accactgcca gaagattctc gccgcgcaac ccgaccacgc
     3481 gggggctttg cactgcgcgg cgaatgcgtc ggagtcgctc ggcgaacggc aacggggcga
     3541 gggctatcgc agaatcgccg ccgcgctcgg cgggacgccg gacgcgaagg ccgcgcaggg
     3601 agatgagtcg aagccgctgg cagaggcgtt ggaagagatc ttcggccccg acacaggccg
     3661 ccgcgacgac aagggggaag gcaaagccgt gccgcgccgc gtggttgacg gagacactac
     3721 cgcagggcac tgggaggccg aaccgtctga catcaagctc tcggacgtgg cgggaatgga
     3781 ggaggtgaag cgtcgccttc atctctcgct gctcgcgccg ctgcgcaacc ctgagatgat
     3841 gaagctctac ggcaagtcgc tgcggggcgg gctgctgttc tatggtccgc cgggctgcgg
     3901 caagaccttt atcgcgcgtg cggtcgcggg cgagatgggg gcgcgcttca tcgccatcgg
     3961 gctggccgac gtgctcgaca tgtacatcgg ccagagcgag cggaacctgc acgaaatctt
     4021 cgagacggcg cgacgcaccg cgccgtgtgt gctcttcatt gacgagatag acgctctcgg
     4081 tcgcaagcgc agcctgcaac gccactccgg cggcagggac gttgttaacc aactgctctc
     4141 cgaactggac agcatcggca acgacaatcg cggcgtcttc gtcctcgccg cgaccaatca
     4201 cccttgggat gtggacaccg cgctgcgccg tcccggcagg ttcgaccgca cgcttctcgt
     4261 gctcccgccc gaccagaccg cgcgtgccgc catcctccgt tatcacctcg gcgaacgccc
     4321 cgtcgaaggc gtcgacctgc gatgggtggc cgaaaagacc gacggactct ccggcgcaga
     4381 cctcgcgtac gtcgtcgagt cggcaaccga attggcgttg gaagagtcca tctcaaccgg
     4441 caagccccgc cccatccgcc agaacgactt caaacgcgcg ctcaaggaga ttaaaccgag
     4501 catccgccca tggtttgaag tggcgcgcaa ttacgccatg ttctccaacg agggcggcat
     4561 gtatgacgac ctgctcgatt acctgcgggc gaagcgaatg ctctagcagc gcgtgcggcg
     4621 aactccgctg gtaagcgata tacaggattc tgcgtcgagt gaaattttca catgagtagt
     4681 gacaactcct gctcctacgt tcctcattcg cgttgaggct ttcagacaag cgcacactct
     4741 tcaccctttg aatttatgaa cgaagaatcc ttcatcaacc acgcgggcgg gcatgtcgag
     4801 cgtgcgcgca tcctgattga gcaggaccgc tatgcgttgg ccgagtccga actgcgccat
     4861 cacattctcg acgagcctga ggacgcaacg gcgcacgcgt tgctcgcgct tacgctgtcg
     4921 cagcagaata agcacaggga ggcggtggag tcggcgcgcc acgggataca cctcgcgccg
     4981 atgttccctt acgtgcacta cattctggca cacgtctaca accagcagga tcaactcgac
     5041 ggggcggaga aggcgatcaa cgaggctatc cggctcgacc cggaagaggc cgattatttc
     5101 gcgttgctga gtaacgtcaa gttgcggcga cgccgctggc aggaggcgct cgaagctgcg
     5161 gaaacgggac tctatttcaa ctcggagcac gtcggctgca tcaacctgcg ggcgatggct
     5221 ctcaaccaga tggggatgtc cgacgaggcg gcggtcgcca tcgagggcgc gctcagcgtc
     5281 gagccggaga acgcgctcac gcacgccaat cgcggctggc aggagttgca tcgcggcaac
     5341 tac
//
LOCUS       DASWNS010000453         4443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_251163, whole genome shotgun sequence.
ACCESSION   DASWNS010000453 DASWNS010000000
VERSION     DASWNS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251163"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2046)
                     /locus_tag="VGB76_15645"
     CDS             complement(<1..2046)
                     /locus_tag="VGB76_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0100379.1"
                     /translation="MSEVSLQGFAISPQQQRLWRLHQDGSNYTSGCAIVLEGHVEPDV
                     LKDALRRIVLRHEVLRTGFYHPPALTFPLQVIADEATFEWRALDLQNLERAQQAERID
                     ALLQEQLRLPFDLERAQPLRAILATLSVERYVLLLTLPALYADSATLKNLFNELGKSY
                     QVNPPDGDSPDEVMQYTQFSEWQNSLLEEVDETSIQHWRQHDLSNQSPLPLELAPAEL
                     PKYKSEMLSVEIEPPVFDRLQAVARRHNTETSTFLLACTHVLLRRLTGQPQVIVGVTL
                     DGRKFEPLLESFGLFAKTVPVQAQFERNSHFSEILVQLDESLQQAHSFQEYFDWVQDA
                     EKPGEAATSLLSVGFEFEAEPAAYTAGGITFSLHRSYAETDRFKVKLRCVSAGDGLRT
                     EFHYDPAFHTTENIERIARSFKQLVNSAAADPSLPINNLEILSDADRHQLISEWNDTH
                     EDYSQQQRLHELFEEQVERTPDAVAVVYEDTQLSYRELNERANQLAHHLQTHGVAPEM
                     LVGVLMERSLEMVVALLGVLKAGGAYVALNPEYPKQRLSFMIKDARLPVVLSQTWLIE
                     KLPRRQAQIISLDAAQETLDSQSRANPATPVEAEHPAYVIYTSGSTGQPKGVVVTHRA
                     ITNHMRWMQERFPLTHTDRVLQKTPFSFDASVWEFYAPLLCGGQLVMARPGGHRDGAY
                     "
     gene            complement(2061..>4443)
                     /locus_tag="VGB76_15650"
     CDS             complement(2061..>4443)
                     /locus_tag="VGB76_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEY0100380.1"
                     /translation="VSFAQQSLWFLHQLEPDDPGYNVPAAVRLSGPLDVVALERSLNE
                     IIRRHEVLRTTFATLEGETVQVIHTEMQLRIVVQSLQDLPAAEREQEAGKLSLEEARR
                     AFDLMGGAMLRVKLLRTADEEHILLLTMHHIVADGWSMGILIRELATLYEAFSAGQPS
                     PLAELPLQYADYAAWQREWLKGESCAAQLAYWKRQLAGAPAVLEFPTDHAKQPGQSPA
                     GAVKSLVLNKELTDALRAFSQREGVTLFMTLLAAYKVLMYRYSEQQDIIVGTPIANRN
                     RAQIEGLIGFFVNVLALRTKVSGGLRFRELLTQVREVSLQAYAHQDIPFDKLVEVLQP
                     KRKPHQTPIFQTTFVLQNIPLSQTLRLPRLTLAPYEIPLVQMQYDFSITVWEEPEQLT
                     AYLKYLTALFEPETITPIIENYEAILRRIVVEPDVRLNSIEMMGKQERSTPVMESKER
                     ANASLKKLLAVKPKAINIQKANLVRTSYLASGESLPLLIEPVGEMINLAVWAKGNLEF
                     IETELLKHGALLFRGFQADTLNRFEQFVTTISPQLFEYRERSSPRTSIGKQIYTSTDH
                     PADQWIQMHSEHTYSHQWPLKIWFGCLQPAEQGGETPMASNRQVLRSLDPKIIERFEE
                     RKVMYVRNYGDGLGVSWQTAFQTSDRAEVEAYCREAGIRLEWTDANHLRTWQVREAVR
                     RHPKTGEPTWFNHLNIYNVATLDADVQKSLLTMFREEELPFNTYYGDGSKIEPSVLAE
                     IREAYTRAIITFPWELGDVLMLDNMFVAHGRKPYQGSRKIVVAMGDLFGSRDS"
BASE COUNT          864 a         1267 c         1323 g          989 t
ORIGIN      
        1 atacgctccg tcacgatgcc cgcccggacg cgccatgacg agttgcccgc cgcacaacaa
       61 cggcgcgtag aactcccaga ccgaggcgtc gaagctgaaa ggtgtcttct gtaagacgcg
      121 gtcggtgtgg gtgagaggaa agcgttcctg catccagcgc atgtggttgg tgatggcgcg
      181 atgggtgacg acgacgcctt tgggctgtcc ggtcgagcct gacgtgtaga tgacgtaggc
      241 ggggtgctcc gcttcgaccg gcgttgccgg gttggcgcgg ctctggctgt cgagtgtctc
      301 ctgtgcggca tcgagactga tgatctgcgc ctgccgtctg gggagtttct ctatcagcca
      361 cgtctggctc aagaccaccg gaaggcgagc atccttgatc atgaacgaca gacgctgttt
      421 cggatattca ggattcagtg ccacgtatgc gccgcctgct ttgagcactc ccagcagcgc
      481 aacaaccatt tcgagcgaac gctccatcaa cacgccgact aacatctccg gagccacgcc
      541 atgcgtttgc agatgatgtg ccagttgatt ggcacgctcg ttgagttcgc gataagacaa
      601 ctgcgtgtct tcatacacaa cagcgacggc gtcgggcgtg cgctcgactt gttcctcgaa
      661 caactcgtgg agtctctgtt gctgcgaata atcctcgtgt gtgtcgttcc attcggagat
      721 aagctggtgc cggtctgcgt cgcttaatat ttccagatta ttgatgggca gcgatgggtc
      781 tgcggcggca ctgttgacga gttgtttgaa ggaacgcgcg atccgttcga tgttctcggt
      841 cgtatggaaa gccgggtcgt agtgaaattc cgtgcgcagg ccgtcgcccg ccgacacgca
      901 acggagcttg accttgaaac ggtctgtctc ggcatagctc cggtgcagag agaaagtgat
      961 cccgccagcc gtataggcgg caggctccgc ttcaaactca aagcctacgg agagcaacga
     1021 ggtcgcagct tcgccgggct tctccgcatc ctgtacccaa tcgaagtatt cctgaaagct
     1081 gtgggcttgc tgtagcgact cgtcgagttg gacgaggatt tcgctgaaat gcgagttgcg
     1141 ttcaaactga gcctgcaccg gcaccgtctt cgcaaacaag ccgaacgatt cgagtagcgg
     1201 ctcgaacttt cttccgtcga gcgtcacgcc gacgataacc tgtggttgcc ctgtgagacg
     1261 ccggaggagc acgtgcgtgc aggccagcag gaaggtggag gtttccgtat tgtgcctgcg
     1321 ggctacggct tgaagtcggt cgaacacggg aggctcaatc tcgacggaga gcatctccga
     1381 cttatatttc ggaagttcgg caggcgcgag ttccagaggc aagggcgact gattcgagag
     1441 gtcgtgctga cgccagtgct ggatgcttgt ttcgtcaact tcttcgagca ggctattctg
     1501 ccactcggag aactgcgtgt attgcatcac ctcgtccggc gagtcgccgt ccgggggatt
     1561 gacctgatat gactttccga gttcattgaa caggtttttg agagtcgcgg agtctgcgta
     1621 gagcgcgggc agggtcagga gcaggacgta tcgctcgacg gagagggtcg ccagaatggc
     1681 gcgcaggggt tgcgcccgct ccagatcgaa gggtagccgc aactgctctt gaagcagagc
     1741 atctattcgc tccgcctgtt gcgctctctc cagattctgc aagtcgagcg cgcgccactc
     1801 gaaggtggcc tcgtcggcga tcacctgcaa aggaaatgtc aacgcaggcg ggtgataaaa
     1861 ccccgtgcgc aagacctcat gccggaggac gattctccgc agagcgtctt tgagcacgtc
     1921 tggctcgacg tgcccttcaa gcacaatagc gcagcctgac gtatagttcg agccatcttg
     1981 atgtaaccgc cacagacgct gctgttgtgg cgaaatggcg aatccctgga ggctcacctc
     2041 ggacatgctc tctctccctt ttatgagtcg cggctgccga acagatcgcc catggcgacc
     2101 acgatctttc ggctgccctg gtaaggttta cgcccgtgag cgacgaacat gttgtccagc
     2161 atcaggacat cgcccagttc ccacgggaag gttatgattg ctctcgtata tgcttctcga
     2221 atctccgcca gcaccgaagg ctcgatcttc gagccgtcgc cgtaataggt attgaagggc
     2281 agttcctctt ccctgaacat cgtcaacaga gacttctgca cgtctgcgtc gagcgtggcg
     2341 acgttgtaga tgttgaggtg attgaaccag gtcggctcgc ccgttttcgg atgtcgcctg
     2401 acggcttctc gcacctgcca cgtccgcagg tggttcgcat ccgtccattc cagcctgatg
     2461 cccgcctcgc ggcaataggc ttctacttcg gcgcggtcgg aagtttgaaa agcggtctgc
     2521 caggagacgc ccaaaccgtc gccgtaattc ctgacgtaca tcacttttcg ctcctcaaac
     2581 cgctcgatga tcttcgggtc gagtgagcgg agcacctgcc ggttactcgc catcggagtc
     2641 tccccgccct gctcggcggg ctggagacaa ccgaaccaga ttttcagcgg ccactgatgc
     2701 gaataggtgt gttcgctgtg catctgtatc cactggtcag ccgggtggtc tgtcgaggtg
     2761 tagatttgct tgccgatgga ggtgcgcggg gaagagcgtt cgcggtattc aaagagttgg
     2821 ggcgagatcg tcgtcacaaa ctgttcgaaa cggttgaggg tgtcggcctg aaaaccccgg
     2881 aacaggagcg cgccgtgctt taacaactcc gtctcaatga actccagatt ccccttcgcc
     2941 cataccgcga gattgatcat ctctccgacc ggctctatca acagcggcag gctctcgcct
     3001 gacgccagat aactggtcct gacaagatta gccttttgaa tgttgatcgc cttcggctta
     3061 accgccagca gctttttgag actcgcattc gcacgctctt tgctttccat aactggtgtg
     3121 cttctctcct gcttgcccat catctcgatt gagttcagcc gcacgtccgg ctctacgacg
     3181 atccggcgca ggatggcttc gtaattttcg atgatgggcg taatggtttc gggttcgaac
     3241 agggctgtca ggtatttcag atacgccgtc aactgctccg gctcttccca gaccgtgatg
     3301 gagaaatcgt actgcatttg cacgaggggg atttcgtagg gcgcgagcgt gaggcgcggg
     3361 agcctgagcg tttgggagag cggaatattt tgtaagacga aagtggtttg gaagattggc
     3421 gtctgatgcg gtttacgctt gggctgcaac acctctacga gtttgtcgaa ggggatgtcc
     3481 tgatgcgcgt aggcttgcag cgaaacctca cgcacctgcg tcaacagttc gcggaaacgc
     3541 aggccgccgg aaaccttcgt gcgcaacgcc agcacattta caaagaagcc gatcaagcct
     3601 tcaatttgcg cccggttgcg gttggcgatg ggcgtgccga cgatgatgtc ctgctgttcg
     3661 ctgtagcgat acatcaaaac tttgtaggcg gcgagcagcg tcataaacaa cgttacgcct
     3721 tctcgctggc tgaaagcgcg aagcgcatcc gtcagctctt tgttcagtac cagagacttc
     3781 accgcgcccg caggactctg acccggttgc ttcgcatgat ccgtcgggaa ttccagcacc
     3841 gcgggcgcgc ctgccagttg tctcttccaa tatgcaagct gcgccgcgca ggactcgccc
     3901 ttgagccatt cgcgctgcca cgccgcatag tccgcatact ggagcggcaa ttccgccagc
     3961 ggcgaaggct gccctgccga aaaggcttcg tagagcgtcg ccagttccct gatgagaatg
     4021 cccatagacc aaccatcggc gacgatatgg tgcatcgtca gcaggaggat atgttcctcg
     4081 tccgccgtgc gcagcagctt tacacggagc atcgcgccgc ccatgaggtc gaaagcgcga
     4141 cgcgcctctt cgagcgaaag ttttccggcc tcctgttcgc gttcggcggc gggtaaatcc
     4201 tgtaggctct gcacgactat gcgtaactgc atctccgtat ggatgacctg caccgtctcg
     4261 ccttcgaggg tggcaaacgt tgtccgcagc acttcgtgcc gccgaatgat ttcgttgaga
     4321 ctccgctcca gcgcgacgac atcgaggggt ccgctcaggc gcacggcggc gggcacgttg
     4381 tagccgggat cgtccggctc taattgatga agaaaccaga ggctctgctg tgcaaacgag
     4441 acg
//
LOCUS       DASWNS010000454        16688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_146601, whole genome shotgun sequence.
ACCESSION   DASWNS010000454 DASWNS010000000
VERSION     DASWNS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16688
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146601"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2157
                     /locus_tag="VGB76_15655"
     CDS             <1..2157
                     /locus_tag="VGB76_15655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0100381.1"
                     /translation="DPTSLRAVEVLRGPNTAQYGSDGLGGTVQLVSRQPEFGFDRPEW
                     HGEFNNFFTSADLSYGSNALVTYGTKRFGLLTNVMARRVNTLRPGGGIDSHSSITRFL
                     GLPSNITGENRLTDTAFTQYGGTIQMNYAPTNNQQFIVRYQRGQQDGGKRYDQTLGGD
                     GNLIADLRNLMLDFGYVRYLRQGFGFFDNLSATVSYNSQREERVNQGGQGNPLAAITH
                     DKERTTTTGFSFYLDKQLGKRNNFVFGGDLYRDHVNAPSFTFQPVTLANPEIGRSTLT
                     RPRVPNGARYILAGIYVQDAHELIERRLRLSGALRYNVGSYRSRASNSPLVDGQPLFP
                     DDSLRVADFSGRIGAVATLTDGLNLAFNLSRGFRSPNITNLGALGLVGVGFQVSTADI
                     LNLGATVGTTADASAVSSGVQVAPLRSETSNNYELGLRFRRGRFDTELVGFIVDYANS
                     IVRQSLILPAGAEGRTLGSQRIIEQRPNGVVFVEAAPTTPVLVQANFANTRLRGFEYE
                     IDFRLTNEWLFGGNYSYVHAADRESGLSPNLGGGGLPPQLGFLRLRYQPVQKRYWVEA
                     YTMLAGRQDRLSTLDISDRRTGAVRSRAQIQNFFRRGACVRGLTTPGTNGQCLSGGGT
                     LIATGESLRQVQDRVLPIGATINGVFVADDQTPVPLYTAIPGYGLINLRGGYRFNESQ
                     EITLDFENIGDKSHRAPGWGIDGPGRSLTARYQFRF"
     gene            2464..3186
                     /locus_tag="VGB76_15660"
     CDS             2464..3186
                     /locus_tag="VGB76_15660"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0100382.1"
                     /translation="MFRFNKTLTACAIFILLGCAAASNANAAPVVHVTRAAFDAAAPN
                     TNTITFGTTTIDYANSITNFPPVTFAGAANHQVGVHTGSNFGLASGNYALVSNSNTPG
                     QFNVDNIVITLPGGTHAVGFDLKCGNATQVPGVCAGEYAIYVNGALATTVSSNQFNSF
                     SFIGFTAAEEISTIAIRALSGGQPIVDNFSTDAAATVPEPTTMVLFGTGLAGVASFAR
                     KRRRGNGLDDGASDEAEESARS"
     gene            3216..3338
                     /locus_tag="VGB76_15665"
     CDS             3216..3338
                     /locus_tag="VGB76_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100383.1"
                     /translation="METKQVCARLSALTGARAKVFSGKSCAIFSVGFFHASPTI"
     gene            3382..3681
                     /locus_tag="VGB76_15670"
     CDS             3382..3681
                     /locus_tag="VGB76_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100384.1"
                     /translation="MSSFAKQPFCPSSATISAYNAGTLSFLLRRSVAEHLAVCEFCHV
                     VSTLWRSFPADAAAPEMPDAHLVASTAAVPPLPFALRALAENLLGETHTAARDAA"
     gene            3971..9502
                     /locus_tag="VGB76_15675"
     CDS             3971..9502
                     /locus_tag="VGB76_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100385.1"
                     /translation="MKRQLPQRFIAANYFRFALVLFAATCLVAVLMTHKGQAATPASG
                     KLTTANTAANPLRYTAGPFNVPNVTGFAGALQCNAAMPCDDYALDVDVPDGLLDSHQV
                     RIAMSWPNATADFDYRVYLRNADGTAGAEALSIGTSADPEVGVLPAVKRNYLVRVVPF
                     APLGQSVAMTITIESKAGRFNQGAGAAPRYANYPATNGLGTDSGEPTLGANWKTDKVM
                     FIAGLETLRVSFNDAMNPATATWEDKSFLLTSLATLDPILYTDNRTGRTIISQLAGTN
                     SLSALTDDDGETWIPNEGGPLTSGVDHQTIGGGPYHAPLTGGTAVYPNAVYYCSQDLV
                     TAFCARSDDGGLTYGPTVPMYALTCGGIHGHVQVGPDGTVYMPNRQCNSNQGVIVSED
                     NGITWSVRAVADTDTTTSTYDSIPGRWDPAVGVGGGGSVYFGYDNGDGTPHVAVSRDR
                     GQTWVNDQNVGLPYSIRNTAFPTVVAGDDDRAAFAFLGTTEPGSSNNAVWHLYISTTY
                     DGGKTWHTVNATPNNPVQLGTICSDGINCGADRNLLDFMDIQVDRQGRVLVGYADGCL
                     NCTSPAGSRSKHATIARQTCGRRLFKQFDPATDDCGGTGTPTPTPVATPTPLDGNGCV
                     YPGVKVISDATGDQTGAPNANQQQDIESVSVSEDYSGATPSLVFTMKVNQLDPALLTP
                     NASWTTLFNATHADGTSTTYFVSADHNSFDNPAGVAYNYGFVDVSNQNLNTSVGAADS
                     GAMDAAKKTITIKVALNKIKKPVAGTAGATLTGATVDLSAGKRIVAVESRTSLLVGAL
                     GTGLIQTVDQTTTAEYAINGQTACTGGSTPTPTPTPTATPTPTATPTPTATPTPSNDA
                     RCTVPGLPVIGDAAGDQTGAPSANASQDIKSVSIAEQYGAGGIGQLVFTIKVGDLTST
                     LTPNASWRVAFNATHADNTFTTYFVTVANNSNSNPAGVSYNYGFVDTSRNNLNSTVGT
                     ADGGSLDATNDTLTVVLNLNKLKKPVAAATGATLTGATVDLSAGRTLGTVNSATSILV
                     GGLGTGLNVNVDSTGNGNYTMAGAATCPPATSATPTPTPVVGGDAATAARFHNFAAPA
                     GVAEDAGEPSIGVNWKTERTFGNSSGLIPNGGTVNYYGGFLPYMLRVTFDDSVSPAAI
                     KWDQANLVVANAPRVFGDPILFTDSKTGRTFVSQELGLTPLGSTMEFTDDDGRTFTPS
                     TGSGAPSGIDHQTVGGGPFAQPVPAGVNPAYPHGVWYCSQSIADAVCALSLDGGVTFG
                     PSVPMYTIADCAGLHGHIKIGPDGTAYIPNKACGGSLPYHNTGGRQSLILSEDNGLTW
                     KIRPIPQSTTNADRDPSVAIASDSTIYFAYQAADGRSRVVVSRNKGVTWTDDTDIGAP
                     LNVKNSLFHAAVAGDPNRAAVAFYGTETGGSDFDTPGFNGVWYLYISTTYDGGKTWNT
                     VNATPNDPVQRGGICGDGQCRNLLDFFDATIDKRGRVLVGYDDGCVGACVQGGANSFT
                     AKAVIARQSGGRRMFAAYDPPSASAPGAPAVSGSRNGSTVNLSWSTPDNGGATIANYK
                     VYRRNGANVVPLGTATGTTFTDTSYTAGQVYRVTAINNTGEGAYKEFNPSSVTSASAC
                     DGAGVLVVSDVNNDGADLDTQQNTPPDTRVNIKSLHVAEPFLGAGQNKLVFTLKVGGS
                     TVGNTAPPSSQWFIVWNSRQPDANFDRRYVAMKSDAQGNVTYEYGKFGAATNTDTATL
                     PPQNSPYTNQPTRLGDAQGSYDPVNGTITITLPTSNAENVGAGHSLNGLNVRTFFARP
                     DAGIRNQAIASDITGDGSYTLAGNASCAGGTASADNLTLGEQFDSVAWQIAARVINFT
                     L"
     gene            9715..9993
                     /locus_tag="VGB76_15680"
     CDS             9715..9993
                     /locus_tag="VGB76_15680"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0100386.1"
                     /translation="MSGGQKSWERAGDARSAFISYARTLGLDAERFARDMNGESVDSR
                     IVADHERAKSLGVDSTPVIYVNGRKLPPAAQTARDVRAAVESALGSKC"
     gene            10064..10795
                     /locus_tag="VGB76_15685"
     CDS             10064..10795
                     /locus_tag="VGB76_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100387.1"
                     /translation="MRLQSYFTLSLCLLMAASLGCSADSGKAQPPAAPAQTVAGQTTP
                     NTTTASQAASPQTNGSSNVAGVPAQATTTGATSHATAAASPSNGADACALLTSDEIKS
                     VQGEAVLEAKPNRRTDSPFAVTQCFYMTASFTKSVSIEVTRQGASSQTVHDFWKANFE
                     RAEDKREKKNERRKLKGRPLAGEPAKPVKGIGDEAYWISTNANSTLYAFKKDTLIRIS
                     IGGTDNEEARMKKIKTLAEKALARL"
     gene            11206..12642
                     /locus_tag="VGB76_15690"
     CDS             11206..12642
                     /locus_tag="VGB76_15690"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0100388.1"
                     /translation="MRNRVRRSFAVALGLMLSLAAITAQAQKRAAGRNNAATPAKNSF
                     NVPVTYYKLPNGLKVVLSPDTSSPTVAVAVYYQIGFRIEPKERTGFAHLFEHMMFQGS
                     QNLGKMEFIRLVQQNGGILNGSTRFDFTNYFEIMPAHKLETALWAEADRMRGLHITEE
                     NLKNQQGVVGNEVKVGVLNAPYGGFPWLDMPQYANTNWYNAHNFYGDLADIEAAKIEE
                     VHQFFKTYYAPNNAALVVVGDFNEAEARQMVEKYFAAIPSSKLPALPDLSEPRQETER
                     HAVKQDSLAQRPALSFAYHMPPRNTPEFFAMGLLDQILLQGEDSLLNQELVKRRAYTD
                     TVSGGTNLLGNMYNYQGPMLWLGYLFHDAKTKPEEIMSAVDTVMRGVQEKPLDQQMLD
                     RALTKVRSNFYDILGQLNGFGRADLLASFALFDDNPARINTLEAEFRKVTPQIVQRTA
                     REYLRTGNRTVLTIVPKPAAASEAKPAS"
     gene            12682..14094
                     /locus_tag="VGB76_15695"
     CDS             12682..14094
                     /locus_tag="VGB76_15695"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0100389.1"
                     /translation="MNSKKFFALVNAVALGATLFAGALPAPAQQQQKQQPPAGSAPKP
                     FTLPRKETFTLKNGTQVTLVPYGAIPKVTVSAVVLAGNINETAEQVWLADVTGELMKE
                     GTKTRSAGAVAEEAARMGGRLGISVTPDQTSISADVLSESAPQLVALIADVLQRPALP
                     ASELERIKTNFIRNLTIQKSQPGSIAQERFSKLLYADHPYGRIFPTEEMIRNFTIADV
                     EKFYRENFGAARTRIYVAGRFDSAAMRRSITQAFGSWARGSNFVENIPKPVSARKIHL
                     IDRPGAPQSTLYLGLPVVDPSHKDFVALSVTNSLLGGSFASRITSNIREQKGYTYSPN
                     SSLSTHYRDAYWVQVADVTTAVTGPSLKEIFYEIERLQKEPPTEAELQGIKNYRAGVF
                     TIQNSSRQGIIGQLAFLDLHKLPETYLTNYVQNVLAVTPQDVQRIAREYLRSERMAIV
                     VVGDKAQVASQLSDYGEVTY"
     gene            complement(14214..14287)
                     /locus_tag="VGB76_15700"
     tRNA            complement(14214..14287)
                     /locus_tag="VGB76_15700"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14251..14253),aa:Glu,seq:ctc)
     gene            14471..>16688
                     /locus_tag="VGB76_15705"
     CDS             14471..>16688
                     /locus_tag="VGB76_15705"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0100390.1"
                     /translation="MQDTTLESSTKGATDARTQAHADFKLGLVDFNYGDLYQPERLRQ
                     LTAIFYAELRRADAVLHDELITYTKARGANLRGTRAESELLIAVAPHLSRFVARLFNV
                     ETERAAHAESITAQDAVFKFKTFITRRALKRVPPEQALTFDVDDIHGALETLRRAAFP
                     DTLDTDAELGVARMTTRLLAWEERLALKPEAAGADSGGDGGDRESLGEIREARARVAK
                     IEGEVWSLKKFEIDAGEGEAAGGDASFVKAALRLVETWASVHATQGAAKSRVRGWVSF
                     RAPHPLNYEHLVQLERHDADLPERMRGLDANLRRRDGFGLTDPRASEREVLDEVNYCL
                     YCHERDKDSCSKGLHERDGSLKRNPLGIALEGCPLDEKISEMHVLQRDGDSIGALALV
                     MIDNPMCPGTGHRICNDCMKSCIFQKQEPVNIPQAETGVLTDVLKLPYGFEIYSLLTR
                     WNPLHAKRPHALPYNGRNILVVGLGPAGYTLAHYLLNEGFGVVGIDGLKIEPLNPELT
                     GEGGRTVPRAVRDIGEIESPLDKRVLAGFGGVSEYGITVRWDKNFLTMIHLTLARRAN
                     FRFYGGVRFGGTLEIEDAWGLGFDHIAIATGAGRPTIVEMRNNLMRGIRKASDFLMAL
                     QLTGAFKADALANLQVRLPAIVIGGGLTAIDTATELFAYYPVQVEKILARHEELSGEF
                     GEDEIFARFDAEERGVYEEFLAHGRAVRAERERASRAGELPDFVPLVRAWGGVSIVY"
BASE COUNT         3313 a         5728 c         4781 g         2866 t
ORIGIN      
        1 gacccgacct ccctgcgcgc cgtcgaagtc ctgcgtggac ccaacacggc acagtacggc
       61 tcggacggcc tcggcggcac cgtccaactc gtctcgcgcc agcccgagtt cggcttcgac
      121 cgcccggaat ggcacggcga gttcaacaac ttcttcacca gcgcagacct ctcctacggc
      181 agcaacgcgc tcgtcactta cggcacaaag cgtttcggcc tcctgacgaa cgtgatggcg
      241 cgtcgcgtca acacgctcag acccggcggc ggcatcgaca gccactcgtc catcacgcgc
      301 tttctcggtc tgccctcgaa catcaccgga gagaaccgtc ttaccgatac ggccttcacg
      361 cagtatggcg gcacgattca gatgaactat gcgccgacga ataaccagca gttcatcgtg
      421 cgctaccagc gcggccagca ggacggcggc aagcgttacg accagacgct cggcggcgac
      481 ggcaatctca tcgccgatct acgcaacctg atgctcgact tcggctacgt gcgctatctc
      541 aggcagggct tcggcttctt cgataattta tccgccaccg tctcctacaa cagccagcgc
      601 gaagagcgcg tcaatcaggg cggtcagggc aacccgctcg ccgccatcac gcacgacaaa
      661 gagcgcacca ccaccaccgg cttcagcttc tacctcgaca agcaactcgg caaacgcaac
      721 aacttcgtct tcggcggcga cctctaccgc gaccacgtga atgcgccttc cttcactttc
      781 caacccgtca cgctcgccaa cccggagatc gggcggtcaa ccctcacgcg cccgcgcgtg
      841 ccgaacggcg cacgctacat cctcgccgga atttacgtgc aggacgcgca cgaactcatc
      901 gagcggcgtc tgcgcctgag cggcgcgctg cgctacaacg tcggctcgta tcgctcgcgc
      961 gcctcgaaca gcccgctcgt tgacgggcag cctttgtttc ccgacgacag cctgcgcgtg
     1021 gccgattttt ccgggcgcat cggcgcggtg gcgactttaa ccgacggcct caatctcgcc
     1081 ttcaatctca gtcgcggctt tcgttcgccg aacatcacga acctcggcgc gctcggcctc
     1141 gtcggcgtcg gctttcaagt ttcgacggca gacatcctga atctgggcgc gaccgtcggc
     1201 acgaccgccg atgcctccgc cgtctcctcc ggcgtccaag tcgcgccgct tcgttccgag
     1261 acgagcaaca actacgaact cggcctccgc ttccgtcgcg gtcgcttcga caccgaactc
     1321 gtcggcttca tcgtcgatta cgccaacagc atcgtccgcc agtcgctcat cctgcctgcg
     1381 ggcgcggaag ggcgcacgct cggcagccag cgcatcatcg agcaacgacc gaacggcgtc
     1441 gtcttcgtcg aggccgcgcc gaccacgccc gtactcgtgc aggccaactt cgccaacacg
     1501 cgcctcaggg gtttcgagta tgagatcgat tttcgcctga cgaatgaatg gctcttcggc
     1561 ggcaactaca gttatgtgca cgcggcggat cgagagtcgg ggttgtcgcc gaatctcggc
     1621 ggcggcggac tcccgccgca gttgggcttc ctgcgcctgc gttatcagcc ggtgcagaag
     1681 aggtactggg tcgaagccta cacgatgctc gccggacgac aggacaggct ctcgacgctc
     1741 gacatctccg accgtcgcac gggcgcggtg cgctcgcgcg cacagattca aaacttcttc
     1801 cgtcgcggcg catgcgtgcg cggcctgacg actcccggca cgaacggcca atgcctctcc
     1861 ggcggcggca cgctcatcgc caccggcgag tcgctccggc aggtgcagga tcgcgtcctg
     1921 cccatcggcg cgaccatcaa cggcgtcttc gtcgccgacg atcaaacgcc cgtcccgctc
     1981 tacacggcca tccccggcta cggcctcatc aacctgcgcg gcggctatcg cttcaacgag
     2041 tcgcaggaga tcacactcga cttcgagaac atcggcgaca agagccaccg cgcgccgggt
     2101 tggggcatag acgggccggg gcgcagcctc accgcgcgtt atcaattccg tttctaacag
     2161 gcaaacgacg aaggcggaac tatggacgcg gaatgaaggc aactcgcttt cattccgcgt
     2221 tcatcgttcc gccttcatcg tttactttat ctgccttcca cgcgtctcaa gtattcctca
     2281 agtctctccc ggaaaaagcc tcaagtattt ctcaagccgc ccctctacac tgcccccgtt
     2341 cgactcgacg ctcgactgaa atcaaggttt ggcctcgccg cccggagcag ccgcctcgtc
     2401 gcccggcgtt cgacccctta aacggagcgc acccatgccg atcaattcaa ggagagaaag
     2461 agaatgtttc gtttcaacaa gaccctgacg gcctgcgcca tcttcatcct gctcggctgc
     2521 gcggcggctt caaacgcgaa cgccgcgccc gtcgttcacg tgacgcgcgc cgccttcgat
     2581 gcggctgcgc cgaacaccaa caccatcact ttcggcacga cgaccattga ctacgcgaat
     2641 tcgataacca atttcccgcc cgtcactttc gcgggcgcgg ccaatcatca ggtcggcgtc
     2701 cacacgggtt cgaatttcgg actcgccagc ggcaactacg cgctcgtctc caacagcaat
     2761 acgcccggcc agttcaacgt cgataacatc gtcatcacgt tgccgggcgg cacgcacgcg
     2821 gtcggcttcg atttgaagtg cggcaacgcg acgcaggttc ccggagtgtg cgccggggag
     2881 tacgcgatct atgtcaacgg cgcgctcgcc acgaccgttt cgtcgaacca gttcaacagc
     2941 ttcagcttca tcggcttcac ggctgccgaa gagatcagca ccatcgccat ccgcgccctg
     3001 agcggcggcc agccgatagt tgacaacttt tctaccgacg ccgccgccac cgtgcccgaa
     3061 ccgaccacga tggtcttgtt tggaacaggt ctggcgggcg tggcttcgtt cgcgcgcaag
     3121 cgtcgtcgcg gtaacggcct cgacgacggc gcaagcgacg aagcggaaga gagcgcgcga
     3181 agttaaacga ggcgaacgag gatcaactgc gcttgatgga aacgaaacag gtgtgcgccc
     3241 gtttgtctgc cctgacgggc gcacgcgcaa aagttttctc cggcaagtcc tgcgcgatct
     3301 tctctgtcgg gttttttcac gcgtcgccca cgatttagtg ccttgaattt gtcacgtccg
     3361 aacctgaaag gagtaagtgc gatgtccagt tttgccaagc agcccttctg cccctcgtcc
     3421 gccacgatct cggcctacaa cgccggaacg ctctccttcc ttctccgccg gagcgtcgca
     3481 gagcatctcg ccgtgtgcga attttgccac gtggtatcga ccttatggcg ctcgttcccc
     3541 gccgatgccg ccgcgccgga gatgcccgat gcgcatctcg tcgcctcgac agccgccgtg
     3601 ccgccgctgc ctttcgctct gcgcgcgttg gccgaaaacc tgctcggcga gacgcacacc
     3661 gccgcgcgcg acgcggcctg aaactcacgc cgcgtaaacc cgcagggtga aagagataaa
     3721 aaacagagcg cgttgattag cgcgaaagtc tctgttggtt ttgaaataaa agcccgtttt
     3781 cgcggctttt ttcggcgtgc gcctgcctca agtattcctc aagaatttct caagtcacat
     3841 ctctatcctg tacccgttca caagtagctt tgtatggctc gcctcgcagt gtagacgcac
     3901 tacgcataac gcgaaccttg cagggtcgaa attgaaaacc acgttgccgc ccacagaagg
     3961 agatcggaaa atgaaacgtc aactgcccca gcgcttcatc gccgccaact attttcgctt
     4021 cgcgctcgtc ctcttcgccg cgacctgtct ggtcgccgtg ctcatgactc acaagggaca
     4081 ggcggcgact cctgcgagcg gcaagttgac gacggccaac acggctgcga atccgctccg
     4141 ctacaccgcc ggacccttca acgtgccgaa cgtcacagga tttgccggtg cgctccagtg
     4201 caacgcggcg atgccctgcg acgactacgc gctcgacgtt gacgtgcccg acgggctgct
     4261 cgattcgcat caggtgcgca tcgccatgtc gtggccgaac gccaccgccg atttcgacta
     4321 tcgcgtctat ctcagaaacg ccgacggcac ggcgggcgcg gaagccctct ccatcggcac
     4381 ttccgccgac ccggaagtcg gcgtgctgcc cgccgtcaag cgcaactacc tcgtgcgcgt
     4441 ggtgccgttc gctccgctcg ggcagagcgt ggcgatgacg atcaccatcg agtcgaaggc
     4501 gggtcgcttc aatcagggcg cgggcgcggc tccgcgttac gcgaattatc cggcgacgaa
     4561 cggcctcggc accgattcgg gcgagccgac gctcggcgca aactggaaga cggacaaagt
     4621 gatgttcatc gccggtctgg aaactctgcg cgtctcgttc aacgacgcta tgaaccctgc
     4681 gaccgccact tgggaagaca agagttttct tctgacctcg ctcgccacgc tcgacccgat
     4741 cctctacacc gacaaccgca ccgggcgcac catcatctcc caactcgccg gaacgaacag
     4801 cctctcggct ctcaccgacg acgacggcga gacgtggatt ccgaacgaag gcggcccgct
     4861 cacttccggc gttgaccacc agaccatcgg cggcggcccc tatcacgcgc cgctcaccgg
     4921 gggcacggcg gtctacccga acgccgtcta ttattgctcg caggatttgg tcacggcctt
     4981 ctgcgcgcgc tccgacgacg gcggcctgac ctacggcccc accgtcccga tgtacgcgct
     5041 cacctgcggc ggcattcacg gccacgtgca ggtcggcccc gacggcacgg tctacatgcc
     5101 gaatcgccag tgcaactcga atcagggcgt gatcgtctcc gaagacaacg gcatcacctg
     5161 gtcggtgcgc gccgtggccg ataccgacac gaccacttcc acctacgaca gcatccccgg
     5221 cagatgggac ccggcggtcg gcgtcggtgg cggcggctcc gtctatttcg gttacgacaa
     5281 cggcgacggc acgccgcacg tcgccgtctc gcgtgatcgc ggccagacgt gggttaacga
     5341 tcaaaacgtc ggtcttccct acagcatacg caacacggcc ttcccgaccg tcgtggccgg
     5401 agacgacgac cgcgccgcct tcgccttcct cggcacgacc gaacccggct caagcaacaa
     5461 cgccgtctgg catctctaca tctcgaccac ctacgatggc ggcaaaacgt ggcacacagt
     5521 caacgccacg ccgaacaatc ccgtccaact cggcaccatc tgctccgacg gcatcaactg
     5581 cggcgcagac cgcaacctgc tcgacttcat ggacattcag gtggacaggc aggggcgcgt
     5641 ccttgtcggc tacgccgacg gttgcctcaa ttgcaccagc cccgccggtt cgcgctcgaa
     5701 gcacgccacc atcgcccgcc agacctgcgg gcgacgcctc ttcaaacagt tcgacccggc
     5761 gaccgacgat tgcggcggca ccggcacgcc caccccgacc ccggttgcca ctcccacgcc
     5821 gctcgacggc aacggctgcg tctaccccgg cgtgaaggtc atctcggacg cgacgggcga
     5881 ccagaccggc gcgccgaacg ccaaccagca gcaggacatc gaatccgtct ccgtctcgga
     5941 agactattcg ggcgcaacgc cctcgctcgt cttcacgatg aaggtcaacc aactcgaccc
     6001 tgcgctctta acgccgaacg cttcttggac gacgctcttc aacgccacac atgccgacgg
     6061 aacttcgacc acctacttcg tcagcgcaga ccacaactcg ttcgacaacc ctgcgggcgt
     6121 cgcctacaac tacggcttcg tcgatgtgtc gaaccagaat ttgaacacct cggtcggcgc
     6181 ggcggactcc ggcgcgatgg acgcagcgaa gaagacgatc acgatcaagg tcgcgctcaa
     6241 taagatcaag aagcccgtcg ccggaaccgc cggtgcgacg ctcacgggcg cgacagtcga
     6301 tctctccgca ggcaagcgca tcgtcgccgt cgagagtcgc acgagccttc tcgtcggcgc
     6361 gctcggcacc ggcctcatcc agacggttga ccagacgacc acagccgagt acgccatcaa
     6421 cggccagacg gcctgcaccg gcggcagcac gccgaccccg acaccgacgc caaccgcgac
     6481 tccgaccccg accgcgactc cgacaccgac tgctacgccg acgccttcaa acgatgcgcg
     6541 ctgcaccgtg cccggcctcc ctgtgatcgg cgatgcggcg ggcgaccaga cgggcgcgcc
     6601 cagtgcgaac gcgtcgcagg acatcaaatc cgtctccatc gcggagcagt acggggcggg
     6661 cggcatcggc caactcgtct tcaccatcaa ggtcggcgac ctgacttcca cgctgacgcc
     6721 gaacgcttcg tggcgcgtcg ccttcaacgc aacgcacgcc gacaacacgt tcaccaccta
     6781 cttcgtgacc gtcgccaaca actcgaactc caacccggcg ggcgtctcct acaactatgg
     6841 cttcgtagac acgtcgcgca acaatctcaa ctccacggtc ggcacagccg acggcggctc
     6901 gctcgatgcg acgaacgaca cgctgaccgt cgtgctcaat ctcaacaaac tgaagaagcc
     6961 cgtcgccgcc gccaccggcg cgacgctcac gggcgcgaca gtcgatctct ctgcgggcag
     7021 gacgctcggc acggtcaaca gtgcgaccag catcctcgtg ggcggactcg gcaccggcct
     7081 caacgtcaac gtcgattcca ccggcaacgg taactacacg atggccggtg cggccacctg
     7141 cccgcccgcc acgagcgcga cgcccacgcc gacacccgtt gtaggcggcg acgcggccac
     7201 agccgcgcgc ttccataact tcgccgcgcc tgcgggcgtc gccgaagatg cgggcgagcc
     7261 ttccatcggc gtcaactgga agacggagag aactttcggc aacagcagcg gcctcatccc
     7321 gaacggcggc acggtcaact actacggcgg cttcctgccc tacatgctgc gcgtcacctt
     7381 cgacgattcg gtgtcgcccg ccgcgatcaa gtgggatcag gccaacctcg tcgtcgccaa
     7441 cgccccgcgc gtcttcggcg acccgatcct gttcaccgat tccaagaccg ggcgcacctt
     7501 cgtctcacag gagttaggtc tgacgccgct cggcagcacg atggaattca cggacgacga
     7561 cgggcgcacg tttacgcctt ccacgggttc gggcgcgccc tccggcatcg accaccagac
     7621 ggtcggcggc ggccccttcg cgcagcccgt cccggcgggc gtcaatcccg cctacccgca
     7681 cggcgtctgg tactgctcgc agtccatcgc cgatgctgtg tgcgcgctct cgctcgatgg
     7741 cggcgtcacc tttggtccca gcgtgccgat gtacaccatc gccgactgcg ctggcctgca
     7801 cggccacatc aagatcggtc ccgacggcac ggcctacatt ccgaacaagg cgtgcggcgg
     7861 cagcctcccc tatcacaaca cgggcggcag gcagtcgctc atcctctcgg aagacaacgg
     7921 cctgacgtgg aagattcgac cgatcccgca gtccacgaca aacgccgacc gcgatccttc
     7981 cgtcgccatc gcctccgata gcaccatcta cttcgcctat caggcggctg acggtcgctc
     8041 acgcgtcgtc gtctcgcgca acaagggcgt gacgtggacg gacgacacgg acatcggcgc
     8101 gcccctcaat gtgaagaaca gcctcttcca cgcggcggtc gcaggcgacc cgaaccgcgc
     8161 cgccgtcgcc ttctacggca cggagacggg cggctccgat ttcgacacgc ccggtttcaa
     8221 cggcgtctgg tatctctaca tctcgaccac ctatgacggc ggcaagacgt ggaacaccgt
     8281 caacgccacg ccgaacgatc ccgtccagcg cggcggcatc tgcggcgacg gtcaatgccg
     8341 caacctgctc gacttcttcg acgccacgat tgacaagcgt ggtcgcgtgc tcgtcggcta
     8401 cgacgacggc tgcgtcggcg cgtgcgtgca gggcggcgca aactccttca cggctaaagc
     8461 cgtcatcgcg cgccagtccg gaggccgccg catgttcgca gcctacgatc cgccgtccgc
     8521 ttccgctccg ggcgcgcccg cagtctccgg cagccgcaac ggttcgaccg tcaacctctc
     8581 atggagcacg cccgacaacg gcggcgcgac gattgcgaac tacaaggtct accgccgcaa
     8641 cggcgcgaac gtcgtgccgc tcggcacggc cacgggcaca acctttacag acacttctta
     8701 cacggcgggt caggtctatc gcgtcaccgc catcaacaac acgggcgagg gagcttacaa
     8761 ggagttcaac ccgtcgagcg tgacgagcgc gagcgcgtgc gacggcgcgg gcgtcctcgt
     8821 cgtcagcgac gtgaacaacg acggcgcaga cctcgacacg cagcagaaca cgccgcccga
     8881 cacgcgcgtc aacatcaagt cgcttcacgt cgcagaacct ttcctcggcg cgggtcagaa
     8941 taaactcgtc ttcacgttga aggtcggcgg ctcgactgtg ggtaacaccg cgccgccttc
     9001 gagccagtgg ttcatcgtct ggaacagccg ccagccggac gccaacttcg accgccgcta
     9061 cgtcgccatg aagtccgacg cgcagggcaa cgtcacctac gagtacggca agttcggcgc
     9121 ggccaccaac acggacacgg cgacgcttcc gccgcagaac agcccgtaca ccaaccagcc
     9181 gacgcgactc ggcgacgcgc agggcagcta cgatcctgtg aacggcacga tcaccatcac
     9241 gctcccgacc tcgaacgccg agaacgtcgg cgcgggacat tccctgaacg gcctgaacgt
     9301 gcgcaccttc tttgcgcgtc cagacgcagg catacgcaat caggccatcg cctccgacat
     9361 cacgggcgac ggctcctaca cgctcgcggg caacgcctcc tgcgcgggcg gcacggcctc
     9421 ggcggacaac ctgacgctcg gagagcaatt cgattccgtc gcgtggcaga tcgcggcgcg
     9481 tgtcatcaac ttcacgctct gagcgtcggg gagcgaatgt tcacgcgtga acgccgctcc
     9541 ggtgcatcag gcaatctcaa ggctcgcgtc gctccggctg aacgacgcga gccatcattt
     9601 aatataagga tgaaggatga atataagaga accgcttgag cggttgaatg ctttcattca
     9661 tcgttcatcc ttctgctttc atcgttctct tcagttgatg gtaggatacg cgccttgagt
     9721 ggcgggcaga aatcgtggga gagagcgggc gacgcacgct cggcgtttat cagttacgca
     9781 cgcacgctcg gcctcgacgc cgaacgcttc gcacgcgaca tgaacggcga gagcgtggac
     9841 tcgcgcatcg tcgccgatca cgaacgcgcc aaatcgctcg gcgtggacag cacgcccgtc
     9901 atctacgtca acgggcgcaa actcccgccc gccgcgcaga cggccagaga cgtgcgcgct
     9961 gccgtcgaga gcgcgctcgg cagcaaatgc tgaacgcgcc gttgcgtgtt gttgacatta
    10021 ccgtttgaca gttatccacg aatcatcgga ggaagtttcc ggcatgcgcc ttcaaagcta
    10081 tttcacactt tcgctctgcc tgctcatggc cgcgtcgctc ggttgtagcg ccgattccgg
    10141 caaggcgcag ccgcccgccg cgcccgcaca gaccgtagcc gggcaaacga cgccgaacac
    10201 gacaactgcg tcgcaggccg cttcgccgca aacgaacggc agttccaacg tcgcgggcgt
    10261 gcccgctcag gcgacgacga caggcgcaac ttcgcacgcg acggcggcag cgtctccctc
    10321 taacggcgca gacgcctgcg cgctgctcac ttcggacgag atcaagtctg tgcagggcga
    10381 agccgtcctt gaggcgaagc cgaatcgccg caccgattcg cctttcgccg tcacgcaatg
    10441 cttctatatg acggcgagct ttaccaagtc cgtcagcatt gaagtgacgc ggcagggcgc
    10501 gagcagccag accgtgcacg acttctggaa ggccaacttc gagcgcgccg aagacaagcg
    10561 cgagaagaag aacgagcggc gaaaactgaa aggcagaccg cttgcgggcg aaccggccaa
    10621 acccgtcaaa ggcatcggcg acgaagccta ctggatcagc accaacgcca acagcacgct
    10681 ctacgctttc aaaaaagata ccctcatccg catcagcatc ggcggcacgg acaacgaaga
    10741 ggcgcgcatg aaaaagatca aaacgctcgc cgaaaaagcc ctcgcccgcc tctgacttta
    10801 aagaccgccc gcgccagccg cgacgaatga aagccgcgcc agcgactttc aaatggctgc
    10861 cgctagcgcg gctttcactc gtcgcagctc atgccgctcc tgatcgtcgc cgcccgcgcg
    10921 gcagttcaca gtcgcgcttg cgcttcgagg ccgcgacgtt gaaactttaa gcgcacgctt
    10981 tccgttagca cacttgcttt gtaaaatagg cgcgcaaact ttaattcaac cgcccggcgt
    11041 tgcatcctga aaatgagacg cgcccatccc tctgcgcgca tccggcatgc cggtggttga
    11101 tgccttaacg ctcaaaagta aacggtggtc ttagaacggc gcgcttgccg gtcgagcgtg
    11161 tccgttcaag ttataaatcc ggtcaatatc aagtttggag gttctatgag aaatcgcgtt
    11221 cgccgctcct tcgccgtggc tctcggcctg atgctctcgc tcgccgccat cacggcgcag
    11281 gcgcaaaaaa gggctgctgg ccgcaacaac gccgccacgc ccgcgaagaa cagcttcaac
    11341 gtgcccgtca cttattacaa actgccgaac ggcttgaagg tcgtgctctc gcccgacacg
    11401 agttcgccca cggtggccgt cgccgtctat taccagatcg gttttcgcat cgagccgaag
    11461 gagcgcacgg gcttcgcgca cctcttcgag cacatgatgt ttcaaggctc gcagaacttg
    11521 ggcaagatgg agtttatccg cctcgtccaa cagaacggcg gcatcctgaa cggctcgacc
    11581 cgtttcgact tcaccaacta ttttgagatc atgcccgcgc acaaactgga aaccgctctc
    11641 tgggcggaag ccgaccgcat gcgcggactc cacatcacgg aagagaattt gaagaaccag
    11701 cagggcgtcg tcggcaatga ggtcaaggtc ggcgtcttaa acgcgcctta cggcggcttc
    11761 ccctggctcg acatgccgca gtacgccaac accaactggt acaacgcgca caacttctac
    11821 ggcgacctcg cagacatcga agccgccaag atcgaagagg tgcaccagtt cttcaagacc
    11881 tactacgcgc cgaataacgc cgcgctcgtc gtcgtcggcg acttcaacga agcggaagcg
    11941 cgtcagatgg ttgaaaagta tttcgccgcg atcccttctt cgaagctgcc cgcgctgccc
    12001 gacctaagcg agccgcgaca ggagacggaa cgccacgccg tcaaacagga ctcgctcgcg
    12061 caacgccccg cgctctcttt cgcctatcac atgccgccgc gcaacacgcc cgaatttttc
    12121 gccatgggct tgctcgacca gatcctcttg cagggtgaag acagcctgct caatcaggaa
    12181 ctcgtcaagc gtcgcgccta cacggataca gtctccggcg gcaccaacct gctcggcaac
    12241 atgtacaact atcaaggccc catgctctgg ctcggctacc tctttcacga tgcgaagacg
    12301 aagccggaag agatcatgtc ggcggtggac acggtcatgc gcggcgtgca ggagaagcct
    12361 ctcgaccagc agatgctcga ccgcgccctg accaaagtgc gctccaactt ctacgacatc
    12421 ctcggccaac tcaacggctt cggacgcgcc gacctgctcg ccagtttcgc gctcttcgac
    12481 gacaaccccg cgcgcatcaa cacgctcgaa gcggagtttc gcaaagtgac gccgcagatc
    12541 gtgcaacgca cggcgcgcga atatttgcgc acgggcaatc gcacggtgct caccatcgtc
    12601 ccgaagcccg ccgccgcgag cgaagccaag cccgccagct aaaggccgac agtcaaagca
    12661 gactcgaatg gagattttca catgaactcg aaaaagtttt tcgccctcgt caacgcagtc
    12721 gctttaggcg ccaccctctt cgccggtgcg ctgcccgcgc ccgcgcaaca gcaacagaag
    12781 cagcaaccgc ccgcgggcag cgcgccgaag cctttcaccc tgccgcgcaa agagactttc
    12841 actttgaaga acggcacgca ggtcacgctc gtcccttacg gcgcgatccc gaaagtcacg
    12901 gtcagcgcgg tcgtgctggc aggcaacatc aacgagacgg ctgaacaggt ctggctcgcc
    12961 gacgtgaccg gcgaactgat gaaggagggc acgaagacgc gttctgccgg agccgtcgcc
    13021 gaagaagcgg cgcgcatggg cggtcgcctc ggcatctccg tgacgccgga tcaaacttca
    13081 atcagcgcag acgtgctctc cgagtcagca ccgcaactcg tcgcgctcat cgcagacgtg
    13141 ttgcaacgtc cagccctgcc cgcttcggaa ctcgaacgca tcaagaccaa cttcatccgc
    13201 aacctgacca tccagaaatc gcagcccggc tccatcgcgc aggaacgctt cagcaaactg
    13261 ctctacgcag accaccctta cggtcgcatc ttcccgaccg aagagatgat cagaaatttc
    13321 accatcgccg acgtggagaa gttttataga gagaacttcg gcgcggcgcg cacgcgcatc
    13381 tacgtcgccg gacgcttcga ctcggcagcc atgcgtcgca gcatcacgca agccttcggc
    13441 tcctgggcgc gcggctcgaa cttcgtggaa aacatcccga agcccgtctc cgcacgcaag
    13501 attcatttga tcgaccgtcc gggcgcgccg cagtcaacgc tctacctcgg cctccccgtc
    13561 gtcgatccga gccacaagga tttcgtcgct ctctcggtca cgaactccct gctcggcggc
    13621 tcgttcgctt cgcgcatcac ctcgaacatc cgcgagcaga agggttacac ctattcgccc
    13681 aacagttcgc tctcgacgca ctaccgcgac gcctactggg tgcaggtcgc agacgtgacc
    13741 actgccgtca ccggcccctc gctcaaagag atcttctacg agatcgagcg gctgcaaaaa
    13801 gaaccgccca cggaggccga gttgcagggc atcaagaatt accgcgcggg cgtcttcacc
    13861 atccagaact cctcgcgtca gggaatcatc ggccaactcg ccttcctcga cctgcacaaa
    13921 cttccggaga cttacctgac caactacgtg cagaacgtcc tcgccgtcac gccgcaggac
    13981 gtgcaacgca tcgcacgcga atacctgcgc agcgaacgca tggctatcgt cgtcgtcggc
    14041 gacaaagcgc aagtcgccag ccagctttcc gactacggcg aagtcactta ctaaaccgcg
    14101 acggcgcgcg ccgccatgag aggttggcaa aaacgtcagc cgcagcccgc cagacgcgaa
    14161 aaaggttaag gccaccgaga gcatcggcgg ccttaacctt tttcagatca aaatagcgct
    14221 aacggggatc gaacccgtac tctctgcctt gagagggcag cgtgttaacc aattacacca
    14281 tagcgccgca aggacgctgg aattataggt ttgccttcgc gcagttggca agcgcggctg
    14341 cgatgctata ctttcgtata aaggcgcgta gcaggcagtc cgtaaaaatt gccgggttag
    14401 tgggcggcgt gtgccgtgcc ttgaaagtct ctccttttga tattttatcg ctcatttcac
    14461 aattgaactt atgcaggaca ctaccctcga aagcagcacg aagggcgcga ccgatgcgcg
    14521 aacgcaggcg cacgcggatt tcaaactcgg cctcgtcgat ttcaactacg gcgacctcta
    14581 ccagcccgaa cgcctgcgcc aactgaccgc aattttctac gcggaactgc gccgcgccga
    14641 cgccgtgctc catgacgaat tgataactta cacgaaggcg cgtggggcaa acttgcgtgg
    14701 gacgcgtgcc gagtcggaac tgctcatcgc cgtcgcgccg cacctgtcgc gtttcgtcgc
    14761 acgcctgttc aatgtcgaga cggaacgcgc ggcgcacgcg gaaagcatca cggcgcagga
    14821 cgcggtcttc aagttcaaga cgttcatcac gcggcgcgct ttgaaacgcg tcccgcccga
    14881 acaggcttta acgtttgacg tggacgacat tcacggcgcg ctcgaaactc tacgccgtgc
    14941 ggccttcccc gacacgctcg acacggacgc ggaactcggc gtcgcgcgca tgaccacgcg
    15001 tctgctcgca tgggaagagc gtctcgcctt gaagccggaa gcagcgggcg cggacagcgg
    15061 cggcgacggc ggcgaccggg aaagtctcgg cgagatcagg gaagcgcgcg cgcgcgtggc
    15121 gaaaatcgag ggggaagtct ggtcgctgaa gaagtttgag attgatgctg gcgaagggga
    15181 ggcggcgggt ggggatgcgt cgtttgtgaa ggcggcgttg aggctcgtcg agacgtgggc
    15241 gtcggtgcat gcgacgcagg gagcggcgaa gtcgcgcgtg cgcgggtggg tcagttttcg
    15301 cgcgccgcat cctttgaact atgaacatct cgtgcagctt gagcgtcatg atgcagacct
    15361 gccggagcgt atgcgcggcc tcgatgcgaa cttgcgtcgg cgcgacggct tcgggttgac
    15421 cgacccgcgc gcgagcgagc gcgaagtgct ggatgaagtc aactactgtc tctattgcca
    15481 cgagcgcgac aaggattcct gctcgaaggg cttgcacgaa cgcgacggtt cgttgaagcg
    15541 taacccgctc ggcatcgcgc tcgaaggctg cccgctcgac gagaagatct cggagatgca
    15601 cgtcctgcaa agagacggcg attctatcgg cgcgctcgcg ctcgtgatga tcgacaaccc
    15661 gatgtgtccg ggcacggggc atcgcatctg caacgactgc atgaagtcgt gcatcttcca
    15721 gaaacaggag ccggtcaaca ttccgcaggc cgagacgggc gtattgacgg acgtgctcaa
    15781 actgccctac ggctttgaaa tctattcgct tctcacgcgc tggaatccgc tccacgcgaa
    15841 acgcccgcac gcgctccctt acaacggaag aaatattctc gtcgtcggcc tcggtccggc
    15901 aggctacacg ctcgcgcact atctcttgaa cgaaggtttc ggcgtcgtcg gcatcgacgg
    15961 tttgaagatc gaacctttga accccgaact gacgggcgaa ggcgggcgca cggtgccgcg
    16021 cgccgtgcgc gacataggcg agatcgagtc gcctttagat aagcgcgtgc tcgcgggctt
    16081 cggcggcgtc tccgagtacg gcatcacggt gcgttgggac aagaattttc tcacgatgat
    16141 tcatctcacg ctcgcgcgcc gcgcgaattt tcgcttctat ggcggcgtgc gtttcggcgg
    16201 gacgcttgag atcgaagacg cttggggact cggtttcgac cacatcgcga ttgcgacggg
    16261 cgcgggtcgc ccgaccatcg tggagatgcg aaacaatctc atgcgcggca ttcgcaaggc
    16321 ttcggatttt ctgatggcgc tgcaactgac gggcgcgttc aaagccgacg cgctcgccaa
    16381 tctgcaagtg cgcctgcccg caatcgtgat cggcggcggc ctcacggcga ttgatacggc
    16441 gacggagttg ttcgcttatt acccggtgca ggtcgagaag attctggcgc gacacgaaga
    16501 gttgagcggg gagtttggcg aggatgagat tttcgcgcgg ttcgatgcgg aagagcgcgg
    16561 cgtctatgaa gagtttctcg cgcacgggcg tgctgtcaga gcggagcggg agcgcgcttc
    16621 gagggcggga gagttgccgg atttcgtgcc gctcgtgcgc gcgtggggcg gcgtctcaat
    16681 cgtgtatc
//
LOCUS       DASWNS010000455         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_302431, whole genome shotgun sequence.
ACCESSION   DASWNS010000455 DASWNS010000000
VERSION     DASWNS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302431"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VGB76_15710"
     CDS             <1..782
                     /locus_tag="VGB76_15710"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0100391.1"
                     /translation="TGTKLRAHVGSGYRAPSLYERFGSFLSPIVRGTSPGDFTPQFSA
                     LGDPRLSPERSIAFDAGLDQTFARRRAQLSATYFYTRLQEVIAFDFTGLVVQPDPFGR
                     LFGGYVNTGGGLARGVELSFEAAPARSLDVRTSYTYTNADERTPRVGGVIRSFTIPDH
                     QFNLTATQRIGRRLLFNFDFTASSNYLAPVFNPATFGSLVYRFDGIRKADVGANYTLP
                     LGESRSLRFFGYVENLSGREYFENGFRTPGRTGKAGAQFNF"
     gene            801..995
                     /locus_tag="VGB76_15715"
     CDS             801..995
                     /locus_tag="VGB76_15715"
                     /inference="COORDINATES: protein motif:HMM:NF037605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HEY0100392.1"
                     /translation="MNVEPDRRALVEGEDFYREGAVMVFTARYLARRGYCCENGCRHC
                     PYDFRKEKHGDEVSKEQKAG"
     gene            964..1914
                     /locus_tag="VGB76_15720"
     CDS             964..1914
                     /locus_tag="VGB76_15720"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0100393.1"
                     /translation="MKFPKNRRRADRFCALLLLLLALACAGCRDAASVETGGGAQGAS
                     TSTPPSAPQRIISLSPSTTEVLYGVGAFERVVAVSDYDKFPPEVARLPRIGGWSNTNL
                     EQVATLKPDLVIMTSSQAPLIKDKLDALGVRTVTVPSFTVEDALAGIEQIGAVTGREE
                     AARKLLAETRAKLEDVGARTRELPRLRVLCIVDRVPGTLRGLYTATRGSFFSELIEIA
                     GGESIAPPASNGFGQISKEAIVTLNPDIIFDMLQTGEGRMAEDVQEVWRELSNVRAVR
                     DGRVYNLRDESMLHPSQFIGDTARKFAELIHPQAFRAPGQ"
     gene            2095..2892
                     /locus_tag="VGB76_15725"
     CDS             2095..2892
                     /locus_tag="VGB76_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0100394.1"
                     /translation="MSNERGADVLLEARELHFAYAAGLPEVVCGASLSLRRGMLAALI
                     GANGSGKSSLIRLLGGLVRPARGEVFFGGVPLPSLDGRARARRIAYVPQAAASVFPLT
                     ALEVVLMGRSPYTARFRFENERDLAVAHDALAAVDAAHLAARSVTELSGGERQLVALA
                     RALAQEPECLLLDEPSAALDLKHRAGLVRHLSRLRDERGITSLIVTHDLQLLDPAFDQ
                     VFAMRCGTIEAAGTPAEVLRDEVLASIYADEHVRARQAFGRTFVWSE"
BASE COUNT          498 a          895 c          983 g          555 t
ORIGIN      
        1 ccaccggcac gaagctgcgc gcacacgtcg gcagcggtta tcgcgcgcct tcactctacg
       61 aacgcttcgg cagttttcta tcgcccatcg tgcgcggcac ctcgcccggc gacttcacgc
      121 cgcagttctc cgccctcggc gacccgcgcc tcagtcccga acgctccatc gccttcgacg
      181 ccggactcga tcagactttc gcacgcaggc gcgcacaact gtcggccacc tacttttaca
      241 cgcgcttgca ggaagtgatc gcgttcgact tcacaggtct ggtcgtgcag cccgatccgt
      301 ttggccgtct ctttggcggc tacgtcaata cgggcggcgg gctggcgcgc ggcgtcgagt
      361 tgagtttcga agctgcgccc gcgcgcagtc tcgacgtgcg aacctcttac acctacacga
      421 acgcggatga acgcacgccg cgcgtcgggg gcgtgattcg ttccttcacg atcccggacc
      481 accaattcaa cttgacggcg acgcagcgca ttggccgacg cctgttgttc aatttcgact
      541 tcacggcgtc gagcaactac ctcgcgccgg tattcaatcc ggcgaccttc ggcagcctcg
      601 tctatcgctt cgacggcatc aggaaggccg acgtgggcgc gaactacacc ctgccgctcg
      661 gcgagtcacg ctccctgcgc ttcttcggct acgtggagaa tctctccggg cgcgagtatt
      721 tcgagaacgg cttccgcaca ccgggacgga cgggcaaggc gggtgcgcag ttcaattttt
      781 agagcatgat gcgagagagg atgaatgtcg aaccggacag gcgcgcgctc gtggaaggcg
      841 aggattttta ccgggagggc gcggtgatgg tcttcacggc gcgctacctc gcgcgtcgag
      901 gctattgttg cgagaacggg tgccgtcatt gcccctatga tttcaggaag gagaagcacg
      961 gggatgaagt ttccaaagaa cagaaggcgg gctgatcgtt tttgcgcgtt gctgctcttg
     1021 ctgctcgcgc tggcctgcgc cgggtgcagg gatgccgcct ccgtggaaac cggcggaggc
     1081 gcgcaagggg cttcaacttc gacgccgccg agcgcgccgc agcgcatcat ctcgcttagt
     1141 ccgagcacga ccgaggtgct ctacggcgtg ggcgcgttcg agcgcgtggt ggccgtctct
     1201 gattacgata agtttccgcc ggaggtggcg cgtctgccgc gcatcggcgg gtggagcaac
     1261 acgaatctgg aacaggttgc cacgctcaag ccagacctcg tgatcatgac ctcctcgcaa
     1321 gcgccgctca tcaaagataa actcgacgcg ctcggcgtgc gtaccgtcac cgtgccgagc
     1381 ttcacggtcg aagacgcgct ggcaggcatc gagcagatcg gagcggtgac ggggcgcgag
     1441 gaagccgcgc gcaaactgct cgccgagacg cgcgccaaac tcgaagacgt gggcgcgcgc
     1501 acacgcgaac tcccacgcct gcgcgtgctg tgcatcgtgg atcgcgtgcc gggcactctg
     1561 cgcgggctgt acacggcgac gcgcggaagt ttcttctcgg agttgatcga gatcgcgggc
     1621 ggcgagtcca tcgcgccgcc cgcctcgaac ggcttcgggc agatcagcaa agaggccatc
     1681 gtcacgctca acccggacat catcttcgac atgctgcaaa ccggcgaagg caggatggcc
     1741 gaggatgtgc aggaagtctg gcgggagttg tcgaacgtgc gcgccgtgcg cgacgggcgc
     1801 gtctataatt tgcgtgatga gtccatgctc catccttcgc aattcatcgg cgacacggcg
     1861 cgcaagttcg cggagttgat tcacccgcaa gcgttccgcg cgccggggca gtgaggtttc
     1921 cggggcgaac cggaagcgcg cgtccgagtt acactccgcc tcttgcctct cagctttgcg
     1981 cctccggcga gagctaactg tttttcgcgc ggcaagcgca aggtagagag agcgttgggc
     2041 gcagagggtt gaagggtaga cgttgcggaa gcattgatgg ataacgttgc acagttgagt
     2101 aatgaacggg gcgcggatgt tctgctcgaa gcgcgcgagt tgcatttcgc ttacgcggcg
     2161 gggttgccgg aggtggtgtg cggcgcgtcg ctcagcttgc gtcgcgggat gctcgcggct
     2221 ctaatcggcg caaacggttc ggggaagtct tcgctcatcc gcctgctcgg tggactcgtg
     2281 cgtccggcgc gcggcgaagt gttcttcggc ggcgtaccgc ttccgtcgct cgacgggcgc
     2341 gcacgcgcgc ggcgcatcgc ttacgtgccg caggcagcgg cgagcgtctt tcctttgact
     2401 gcgctcgaag tggtcttgat ggggcgcagc ccttacacgg cgcgttttcg ttttgagaac
     2461 gagcgcgatc tggccgtggc gcatgacgcg ctcgcggcgg tggacgcggc gcatctggcc
     2521 gcgcgcagcg tgacggaact ttcgggcggc gagcggcaac tcgtcgcgct cgcacgcgcg
     2581 cttgcgcagg agccggagtg cctgttgttg gatgagccgt cggcggcgtt ggatttgaaa
     2641 caccgtgcgg gactcgtccg ccacctgagt cgcctgcgcg acgaacgcgg catcacttcg
     2701 ctcatcgtga cgcacgatct acaactgctc gacccggcct tcgatcaagt gttcgccatg
     2761 cgctgcggca caatcgaagc ggcgggcaca cccgcagaag tgttgcgcga tgaagtgctc
     2821 gcgtcgatct atgccgacga gcatgtgcgc gcacgtcagg cttttgggcg gacgttcgtg
     2881 tggtcggaat gagattaaag gatgaaggat gagggatgag ggatgaataa g
//
LOCUS       DASWNS010000456         9325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_354739, whole genome shotgun sequence.
ACCESSION   DASWNS010000456 DASWNS010000000
VERSION     DASWNS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354739"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            837..4421
                     /locus_tag="VGB76_15730"
     CDS             837..4421
                     /locus_tag="VGB76_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0100395.1"
                     /translation="MERQRGKEDGRGNSDGNDATEASGSRAGETQPGAAATTNASQPD
                     ASQHASTVAAAQHEGGSERVGGSARKGASPAGGAPSVAELSLAPLVLEMRTTLSELRE
                     TNQTLEAIIHASPLGIIALDSEGIVRMWNNAAERIYGWSEAEVLGQPLPTVPLDKQEE
                     MRRNHQRALSGMDFKAFETERLRKDGTQINVSISTAPLSDAQGQLTGVVALVEDITER
                     KRAEREKSRLAAEVENQRRRLDAIIASVPGVVWEAWGEPDEANQRINFVSDYVETMLG
                     YTVAEWLSTPNFWLNIVHPEDREQAARTAATTFASGEVGTNQFRWIRKDGRAVWVEAH
                     SVALKDAEGRAVGMRGVTMDITERKEAGEALRRSEERYRAFVEQSNEPIWRFELEAPV
                     PVNLPTDEQIERIYAHGYLAECNDVMARMYGYANANDLIGTPLEDLLPRTEANLEYLR
                     DAVKSGYRLLERESQELDRTGQTKYFLNNLVGIVEGGELRRVWGTQRDITERKQAEAE
                     LRRWEQIFQHAGWGVAMAEPESGSLQVVNPAFARMHGQTVKEMLGKPIVETFADEERE
                     KLIDHVRQVHEKGHHVYESIHVRKDGTRFPVLADVTAFKDEHGKVLYRAANIQDITER
                     KRAEEVARFLGDATTMFASSLDYELTLASASRLVVPYMADYCLVHMLEDDGQTIRRMI
                     VAHQNPEREAAWRDMQNRFPLDLNTPHTIAKVLRTGRPEMFSHVTDELMQAVIRNPQE
                     IRMLKEFDIRASMIVPLIARGRTIGTLTFISSESGRHYGADDLALATELARRAALAID
                     NARLYRRAQEANRAKDEFLATLSHELRTPLTPIIGWVHMMSTGQLNADDIAHGLAVIG
                     KNSQSLSRLINDLLDMSAILSGKMRIDSVPVSLESVIKEAIETVRPQASKQRIVIELT
                     PCERMAELVVSGDRTRLVQVCWNLLTNAVKFSPDGSRIRVLCEITNDAAHVHVEDEGE
                     GITPEFIPHVFERFRQQDMSTTKMHGGLGIGLALVKSFVEAHGGTVAVASAGEGRGSR
                     FTLTLPLVPRAQLFTHANDGEAASSCEVDNACHALLIEDAPDTLEMLRVAFEVRGFRT
                     SACATPEEALSIAANEHFDIIISDIGLPNIDGYELLQLLRSQTPYLHDVPALALTGYA
                     AQKDVDAALSAGFSAHLAKPFDPATLAATVDALLKRQQSAPTTGAGER"
     gene            4588..5034
                     /locus_tag="VGB76_15735"
     CDS             4588..5034
                     /locus_tag="VGB76_15735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0100396.1"
                     /translation="MSHLASGGDAYTPNDPSPFSDKPQETKNRPRALVVDDAPDVTEM
                     LAILLQYAGYEVVMVYSGPQALSAARAEQFDVVVSDIGMPGMNGYELASAVRALPAYQ
                     STPMIAVTGFTMYEDRERALSCGFNAFLTKPINPRDLLSLVERLRS"
     gene            5074..6963
                     /locus_tag="VGB76_15740"
     CDS             5074..6963
                     /locus_tag="VGB76_15740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0100397.1"
                     /translation="MPSEESEASILIVNDSPDALALTSVVLQQAGYHVLTATDGLEGL
                     ALARRELPTLIISDVSMPHMDGIELCRRVREDTRMSLTPILLVSAQRKDTESALEGLK
                     AGADDFIEAPFDALRLIAQVARLLERARIEAHYRDIVEQASDIIYTHDLEGLITSINA
                     AGLLFSRRQTEELLGQHLAAAFKLDVAQAEIDELIKRLRSEGVRRVEAQGVNANGERR
                     WLEFNMSLICARDGLPLGVRGVARDITRRKGVEEQLRRSEERYRSLVDNANDIIYTAD
                     MEGRYTSLNKSGERVSGYTLEEVLGMTWKEIVAPDYWPLVSEMLMRKLATQDLVTFYE
                     LEIIAKDGRRIPLEVNSQLIYEDRQPVGMQGIARDITERKHNETLLRARFEQEAESEK
                     MRSLGQLSAGVAHNFNNALAAVLGRTQLLLRSVTDERQRRSLEVIETATLDAAEIVRR
                     IQTFARRAPSAQFAQVSLARLITDAVQLTRTSWEDDAHVHGRHYAVEFTSEVEGVDLI
                     EANATEVREVFVNLLFNALDAMPAGGRIELRETRDDAGFVVTVSDTGEGIPPEFHDRI
                     FEPFFTTKGSQGSGLGLAVSYGIIKRHGGTIEVTSIPAAPSGGADSRGTTFTLKFPHP
                     RQSGQ"
     gene            7016..8899
                     /locus_tag="VGB76_15745"
     CDS             7016..8899
                     /locus_tag="VGB76_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100398.1"
                     /translation="MLFCGLDGRARHALNHSDQGKGRQIFMENAGSHEAAASAAAAAD
                     ALDAVAASDHVAGDRRAGRKVLASASVRRAAYFVTGALAIGFVFWWLQFSTDAICCGD
                     FDGYYHIGWSQQLWQGFRAGRLPQFIWLPLTTLNPRDYVDHHFLFHVLQIPFTFFSDV
                     RTGAKVSAWLFASLAVLACYSMIIRYRIKYPLLWLVALLGCSAPFLFRMSMTKAMSVS
                     IVLLIVGIHLLFQRKYLWLLPLAFFFTLTYDMFVLLGLVAAIWTAVLAWSERRFEWRP
                     LLWVIIGVVAGTVINPYFPHNVQLLYAHIVMKFTSKGFSTAVGQEWYPYESWDFARNC
                     AVAFIAMVAGYIAFNPQERKRTAHALFFLIFSTILLIANARWRRFAEYWPPFAVLFAA
                     FALQLLFERARSVYGSLPEDVLDELEPYLDSPASVPAQRTATATNAWSEAHKTFAAAI
                     ITVLLMVPMVAMIVAETREIETTAPHEKYAKGIEWMRTHIPAGQVIFNTDWDDFPRLF
                     YYDPAHAYVSGLDPTYLYDKDQSLSRLYEEITLGRTKDPAPAIRDRFGARYVFTDNED
                     VHNDFYYAAMESGWFDEVYTDEDCTVLYIRDQKGAPATPEDNPNPDDDNDAADETDEE
                     DAT"
     gene            8922..9302
                     /locus_tag="VGB76_15750"
     CDS             8922..9302
                     /locus_tag="VGB76_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100399.1"
                     /translation="MKVKISKRVSIITLAAFVSVGALGGCDRSGSGDNSAAQNKPSAF
                     EKDLDSVRRNSFQKVYVISRPDGGTLNADDKAYLKTNMPIETTMRILTDEDRRLIVGT
                     NFDFKPEHFDALNKRFRIEDYTGR"
BASE COUNT         1938 a         2988 c         2763 g         1636 t
ORIGIN      
        1 ataaaaggtt cagggttctg agccgaactt caaagcgttg aagcttcgag tcaagcatgg
       61 tcgctcgtgg caggaatatc gcgcagaggt caaactgcgt gttagacagt acgagcagag
      121 agttgaatga gacgcgagcg cgtcccggat tcaggaacca accaggctca agccgttttg
      181 ggtcacgaac taattgaaca cggatggaat aactgctaca ggaattttat ggctgattaa
      241 atttaggtct tattaatgca ggtcgactta tcaagggaaa cggaacagca tcgcggtatg
      301 ccgccgtgtg cgagggggct aagtgcatag gatataacta ccttttcaaa ccttgtgcga
      361 tttagggaag cctcggcttc cgaccggcaa cgaatcgagc aagttaactg acgatgtaaa
      421 cagactattg ggacgggcag gactaaaaac cgataaacct atctaagcgc aagcaaatta
      481 aagaaatcct gcaaagatgt caaggcgtca gcaggctaga acaggatgcc gcgcctcaat
      541 ttgtcgccgt tgtcgctcct ccgaagccgc cgcgaatttc cggcgcggcg gcgcacgctc
      601 ttcgtcacac agcgtgcgcc gtggatgaaa cgcgcgccac gcgcttttaa atcgacggtc
      661 gcggcgcgtg cctttcacgg tgagatttct cacctttgcg cacaacaatc ttgacttgcc
      721 tccgcccgac agcgtagctt tcccttgcgc cggacgcgtt tcctgttatg cgtaattctc
      781 gacggccaga tcaaagaact ctataaactt caattcggaa cagaggcaaa ctgaacgtgg
      841 agcgacagcg cggcaaagag gacgggcgcg gcaacagcga cggtaacgac gcgacggagg
      901 cgtcgggcag ccgcgcgggc gaaacgcaac cgggggcagc ggcgacgacg aatgcgtctc
      961 agcccgacgc ttcgcagcac gcatctacgg tcgccgccgc gcagcatgag ggcgggtctg
     1021 aacgcgttgg cgggtcggcg cgcaagggcg cgtcgccagc gggcggtgcg ccgtctgtcg
     1081 cagagttgtc gctggcgccg ctcgtacttg agatgcgcac cacgctctcc gaattgcgcg
     1141 agacgaatca aacgctcgaa gccatcatcc atgcctcgcc gctcggcatc atcgcgctcg
     1201 actccgaagg catcgtcagg atgtggaaca acgcggcgga acgtatctac ggttggagcg
     1261 aagcggaagt cttaggccag ccgttgccca ccgtcccgct cgataagcag gaagagatgc
     1321 gccgcaacca ccagcgcgcg ctctccggca tggacttcaa agccttcgaa accgagcggc
     1381 tccgaaaaga cggcacgcag atcaacgtca gcatctcgac cgcgccgctc agcgatgcgc
     1441 agggacaact cacaggcgtc gtcgcactcg tcgaagacat caccgaacgc aagcgcgccg
     1501 aacgcgagaa gtctcgactg gcggcggaag tcgaaaacca acgacggcgg ctcgacgcga
     1561 tcatcgcgag cgtgcccggc gtggtctggg aagcgtgggg cgaaccggac gaagccaacc
     1621 agcgcatcaa cttcgtcagc gactacgtgg agacgatgct cggctacacg gtcgccgaat
     1681 ggctctcgac gccgaacttc tggctcaaca tcgtgcaccc ggaggacagg gagcaggcgg
     1741 cgcgcacggc tgccacgact ttcgcgagcg gcgaagtggg caccaaccag tttcgctgga
     1801 tcaggaagga tggccgcgcg gtgtgggtcg aagcgcattc ggtcgcgctc aaggatgcgg
     1861 aggggcgtgc ggtgggcatg cgcggcgtga cgatggacat cacggagcgt aaggaggccg
     1921 gtgaggctct cagacgaagc gaggagcgtt atcgcgcctt cgtcgaacag agcaacgagc
     1981 cgatctggcg tttcgagttg gaagcgcccg tccccgtcaa tcttcccacc gacgaacaga
     2041 tcgaacgcat ctacgcccac ggttatctcg ccgagtgcaa cgatgtgatg gcgcgcatgt
     2101 atggctacgc caacgccaac gatctcatcg gcacaccact cgaagacctc ttgccgcgca
     2161 cggaagcgaa cctcgaatat ttacgcgacg cggttaaatc cggctaccgc ttgctcgaac
     2221 gggaatcgca ggagttggat cgcacggggc agacgaaata ctttctgaac aatctggtgg
     2281 gcatcgtcga aggcggcgag ctgcggcgcg tgtggggcac gcagcgcgac atcacggagc
     2341 gcaagcaggc ggaagcggag ttgcgccgtt gggagcagat tttccaacat gcaggctggg
     2401 gcgtggcgat ggccgaaccg gagagcggca gtttgcaggt cgtcaatccg gccttcgcgc
     2461 gcatgcacgg ccagacggtc aaggagatgc tcggcaaacc catcgtcgag actttcgccg
     2521 acgaagagcg cgagaagctc atagatcacg tgcgtcaggt tcacgaaaaa gggcatcacg
     2581 tctacgagtc gattcatgtg cgcaaagacg gcacgcgctt tcccgtgctg gccgacgtaa
     2641 cggccttcaa agacgaacac ggcaaggtgc tctatcgcgc ggccaacatt caggacatca
     2701 ccgagcgcaa acgcgccgaa gaggttgcac gttttctcgg cgacgcgacc acgatgttcg
     2761 cctcttcgct cgattatgaa ttgacgctgg cgagcgcctc gcgcctcgtc gtgccctaca
     2821 tggccgacta ctgcctcgtg cacatgctcg aagacgacgg ccagaccatt cgccgcatga
     2881 tcgtcgcgca tcaaaaccct gaacgcgaag cggcctggcg cgacatgcag aatcgcttcc
     2941 cgctcgatct caacacgccg cacaccatcg ccaaagtcct gcgcacggga cgaccggaaa
     3001 tgttctccca cgtcacggat gagttgatgc aggccgtgat ccggaatccg caggagatca
     3061 ggatgctcaa ggagtttgat attagagcgt cgatgatcgt gccgctcatc gcgcgcgggc
     3121 gcaccatcgg cacgctgacc ttcatcagtt cggaatcggg gcgacattac ggcgcggacg
     3181 atctcgcgct cgccaccgaa ctcgcgcgtc gcgccgcgct cgccatcgat aacgcgcgcc
     3241 tctatcgccg cgcgcaggaa gccaatcgcg ccaaagacga atttctcgcc accctctcgc
     3301 acgaactgcg cacgcccctg acgcccatca tcggctgggt acacatgatg agcaccggcc
     3361 aactcaacgc ggacgacatc gcgcacggcc tcgccgtcat cggcaaaaac tcgcaatcgc
     3421 tctcccgcct gatcaacgat ctgctcgaca tgtcggccat cctgagcggc aagatgcgta
     3481 ttgactctgt tcccgtgtcg ctcgaatcgg tgatcaagga ggccatcgag acggtgcgcc
     3541 cgcaggcgtc gaagcagaga atcgtgatcg aacttacgcc ctgcgagcgg atggcagagt
     3601 tggtcgtctc cggcgaccgc acgcggctcg tgcaggtctg ctggaacctg ttgacgaacg
     3661 ccgtcaagtt ctcgccggac ggcagccgca tccgtgtcct ctgcgaaatc acgaacgacg
     3721 cggcgcacgt ccacgtcgaa gacgaaggag aaggcatcac gccggagttt atcccgcacg
     3781 tcttcgagcg tttccgccag caggacatgt caacgacgaa gatgcacggc gggctgggca
     3841 tcgggctggc actcgtcaag agtttcgtcg aagcgcacgg cggcacggtc gccgtggcga
     3901 gcgcgggcga agggcgcggc agtcgcttta ctctgacgct gccgctcgtg ccgcgcgcgc
     3961 agttattcac gcacgcaaac gacggggagg ccgcgtcctc ctgcgaagtt gataacgcgt
     4021 gtcacgccct gctcatcgag gacgcgcccg acacgctgga gatgctgcgc gtggcttttg
     4081 aagtgcgcgg ctttcgcacg agcgcgtgcg ccacgcccga agaggctctc agcattgcag
     4141 caaatgagca ctttgatata atcatctccg atattggtct tcccaacatt gacggttatg
     4201 agcttttgca gctactgcga agccagactc cttatctgca tgacgtgcct gcgctcgcct
     4261 tgacgggcta cgccgcgcag aaagatgtgg acgccgccct ctccgcggga ttcagcgcgc
     4321 acctcgccaa gccgtttgac ccggctactc tggccgctac ggttgacgcg ttgctcaaac
     4381 gtcaacagtc cgcgccgacc acaggtgcgg gtgaacgtta gaacaagccc gggcttgtaa
     4441 gacggaagcg caggaaattt tgcctccgct tcgacgcagt actttccagt tttgccgagg
     4501 ccgcgcaagc ggaaagggag agataggaac atgctggttg cccctgaaac tgtcgcgcgc
     4561 gcgaccgccg cgtgcgcagt atttaccatg agccacctcg cttccggcgg agacgcctac
     4621 acgcccaacg atccttcgcc cttcagcgac aagccgcagg agacaaaaaa cagaccgcgc
     4681 gccctcgtcg tagacgatgc gccggacgtg acggagatgc tggcgattct cctccagtac
     4741 gcgggttatg aagtggtgat ggtctactcc gggccgcagg ctctcagcgc ggcgcgcgca
     4801 gagcagttcg acgtggtcgt ctcggacatc ggcatgcccg gcatgaacgg ttacgagttg
     4861 gcttccgccg tgcgcgctct gcccgcttac cagtccacgc cgatgatcgc cgtcacgggc
     4921 ttcaccatgt acgaagaccg cgagcgcgca ctcagttgcg gcttcaacgc attcttgacg
     4981 aaacccatta acccgcgcga cctcctcagc ctcgtcgagc gtctgcgaag ttgaagaggt
     5041 tgagaagcgg tttcgcttta atacggacga acgatgccca gcgaagagtc ggaagcgtca
     5101 attctcatcg tcaatgactc gcccgacgca ctcgcgctca cgagcgtcgt gttgcaacag
     5161 gccggttatc atgtgctcac ggcgacggac ggcctcgaag gtctggcgct cgcgcgccgc
     5221 gaacttccca cgctcatcat cagcgatgtc tcgatgccgc acatggacgg catcgaactg
     5281 tgtcgtcgtg tgcgcgaaga cacgcgcatg tcgctcacgc cgatcctgct cgtcagcgcg
     5341 caacgcaaag acacggagag cgcgctcgaa ggactcaagg ccggagcgga cgactttatc
     5401 gaagctcctt tcgatgcgct ccgcctcatc gcgcaggtcg cgcgcctgct cgaacgcgcc
     5461 cgcatcgaag cccactatcg agacatcgtc gaacaggcca gcgacatcat ctacacgcac
     5521 gacctcgaag gcttgatcac gagcatcaac gccgccggtc tgctcttcag ccgtcgccag
     5581 acggaggaac tgctcggcca gcatctcgca gcggcgttca aattagatgt ggcgcaggcc
     5641 gagattgacg agttgatcaa gaggctgcgg agcgagggcg tgcggcgcgt ggaagcgcag
     5701 ggcgtgaacg cgaacggcga gcgacgctgg ctggaattca acatgtcgct catctgcgcc
     5761 cgcgacggtt tgccgctcgg cgtgcgcggc gtggcgcgcg acatcacgcg gcgcaagggc
     5821 gtcgaagagc aactgcgacg cagcgaggaa cgctatcgca gcctcgtcga taacgcgaac
     5881 gacatcatct acaccgccga catggaaggc cgctacactt cgctcaacaa gtccggcgag
     5941 cgcgtgagcg gctacacgct cgaagaagtt ctcggcatga cgtggaagga gatcgtcgcg
     6001 ccggactact ggccgctcgt cagcgagatg ctcatgcgca agctggcgac gcaggacttg
     6061 gtcaccttct acgaactcga aatcatcgcc aaagacgggc ggcgcatccc gctcgaagtc
     6121 aacagccaac tcatctacga agaccgccaa cccgtcggca tgcagggcat cgcccgcgac
     6181 atcaccgaac gcaaacataa cgagacgctg ctgcgcgcgc gcttcgaaca ggaggccgag
     6241 agcgaaaaga tgcgcagcct cggccaactt tcggcgggcg ttgcgcacaa cttcaacaac
     6301 gcgctcgccg ccgtgcttgg ccggacgcaa ctgctcctgc gctccgtgac ggacgagcgg
     6361 cagcggcgca gcctcgaagt gatcgagacg gccacgctgg acgccgcgga gatcgtgcgc
     6421 cgcattcaaa ccttcgcgcg ccgcgcgccc tccgcacagt tcgcgcaagt ctcgctcgca
     6481 aggctcatca ctgacgccgt gcaactcacg cgcacgagtt gggaagacga cgcccacgtc
     6541 cacggtcgcc attacgcagt ggagttcacg agcgaagtgg agggcgtcga tctcatcgaa
     6601 gccaacgcga ccgaagtgcg cgaggtcttc gtcaacctgc tcttcaacgc gctcgacgcg
     6661 atgcctgcgg gcgggcgcat cgagttgcgc gaaacgcgag acgacgccgg gtttgtcgtc
     6721 accgtcagcg acacgggcga aggcattccc ccggagtttc acgaccgcat cttcgaaccg
     6781 ttcttcacca ccaaaggttc gcagggctcc ggcctcggcc tcgccgtctc ctacggcatc
     6841 atcaaacgcc acggcggcac catcgaagtg acgagcatcc ccgccgcccc ctccggcggc
     6901 gcggacagtc gcggcacgac cttcacgctc aaattccccc accctcgcca gagcggacag
     6961 taaagcgacg ggcgcgcggt tcgcagccgc ccgcttttcc tcccctcacg cttccatgtt
     7021 attttgtggc ctcgacgggc gagcgcgcca tgctctcaac cattcggatc aggggaaggg
     7081 acgacaaatc tttatggaga acgccggaag ccatgaggcc gccgccagtg ctgccgccgc
     7141 cgcagacgct ctcgacgcgg tcgcagcgag cgaccacgtg gccggtgaca ggcgcgcagg
     7201 ccgcaaagtg ctcgcgtctg caagcgtcag gcgcgcggcc tattttgtga cgggcgcgct
     7261 cgccatcggc ttcgtcttct ggtggctgca attttccacc gacgccatct gctgcggcga
     7321 cttcgacggc tactatcaca tcggttggag ccaacaactc tggcagggct ttcgcgccgg
     7381 gcgtctcccg cagttcatct ggctgccgtt gacgacgctc aacccgcgcg actacgtaga
     7441 tcaccatttc ctcttccacg tcctgcaaat tcccttcacc ttcttttccg acgtgcggac
     7501 gggcgcgaaa gtttcggcgt ggctctttgc gagcctcgcg gtgctcgcct gctactcgat
     7561 gatcattcgc tatcgcatca agtatccgct gctgtggctc gtcgcgctgc tcggctgttc
     7621 cgcgccgttt ctgtttcgca tgagcatgac gaaggccatg agcgtctcca tcgtgctgct
     7681 catcgtcggc atccacctgc tctttcaacg aaaatatttg tggctgctgc cgctcgcctt
     7741 cttcttcacg ctgacttatg acatgttcgt cctgctcggc ctcgtggccg ccatctggac
     7801 ggcggtgctc gcgtggagcg agcggcgttt tgaatggagg ccgctcctgt gggtcatcat
     7861 cggcgtcgtc gcgggcacgg tcatcaatcc ctacttcccg cacaacgtcc aactgctcta
     7921 cgcgcatatc gtgatgaagt tcacgtcgaa gggcttctcg acggcggtag ggcaggagtg
     7981 gtatccgtac gagagttggg acttcgcgcg caactgcgcc gtcgcgttca tcgcgatggt
     8041 cgcgggctac atcgccttca acccgcagga gcgcaagcgc acggcgcacg ccttgttctt
     8101 tctcatcttc tccaccatcc ttctcatcgc caacgcgcgc tggcgacgct tcgcggaata
     8161 ctggccgccc ttcgccgtcc tcttcgcagc cttcgcgctg caactccttt ttgagcgtgc
     8221 gcgctccgtc tatggcagcc tgcccgaaga cgtgttggac gaactcgaac cttatctcga
     8281 tagccccgcg agcgtccccg cgcagcggac tgcgacggcg acaaacgctt ggagcgaggc
     8341 gcacaagacc ttcgcggcgg cgatcatcac cgtgctgctc atggtgccga tggtcgccat
     8401 gatcgtcgcc gagacgcgcg agatcgagac gaccgcgcca cacgaaaaat acgcgaaggg
     8461 catcgagtgg atgcgcacgc acatccccgc cgggcaggtc atcttcaaca cggactggga
     8521 cgacttcccc cggctctttt actacgaccc ggcgcacgct tacgtctccg gcctcgaccc
     8581 gacttatctc tacgacaaag atcagtcgct ctcgcgtctc tacgaagaga tcacgctcgg
     8641 cagaaccaaa gaccccgccc ccgccatccg cgaccgcttc ggcgcgcgct acgtcttcac
     8701 cgacaacgaa gacgtgcaca acgatttcta ctatgccgcg atggagagcg gctggttcga
     8761 cgaagtttat acggacgagg actgcaccgt gctctacata cgcgaccaga aaggcgcgcc
     8821 cgccaccccc gaagacaacc ccaacccgga cgacgataac gacgccgcag atgagacgga
     8881 tgaagaagac gcaacttaat gcgaatttta tgtggaacga gatgaaagtt aagatttcaa
     8941 aacgcgtgtc gatcatcacg ctcgcggcgt tcgtctccgt cggcgcgctc ggcggttgcg
     9001 accgctccgg ctccggcgac aacagcgcgg cgcaaaacaa gccctccgca ttcgagaaag
     9061 acctggactc ggtcaggcgc aacagtttcc agaaagtcta cgtcatctcg cgccccgacg
     9121 gcggcacgct caacgccgac gacaaagcct atctcaaaac gaacatgccc atcgaaacca
     9181 ccatgcgcat cctcacggac gaagaccgcc gcctcatcgt cggcacgaac ttcgacttca
     9241 agcccgaaca cttcgacgcg ctcaacaaac gtttccgcat cgaagactat accgggagat
     9301 gaacgcgaga cacgtctcaa ttcgt
//
LOCUS       DASWNS010000457         3664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_227040, whole genome shotgun sequence.
ACCESSION   DASWNS010000457 DASWNS010000000
VERSION     DASWNS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227040"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VGB76_15755"
     CDS             complement(<1..739)
                     /locus_tag="VGB76_15755"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100400.1"
                     /translation="MNACMKLRAGQRAALLFALLALFGSTTGAQNFAPNSKSNSAQTS
                     ALNSTQSRDEQYKINYHLSMPNPASHLFEVRITVETPLVVEAVDFQMPRWSPGRYAVF
                     DFAKNVQEARATTFCAPPAFCDAATLPLTRLDTQTWRVFPGKAQGVALSYKVFANDLS
                     GTFSQLDARHANFNGGSLFMYVVGHKPDPVRLSIDAPAGWRIINSATERADQREWQFP
                     NYDILIDTPTEIAPDFTIDEFKVDGKTY"
     gene            1231..2478
                     /locus_tag="VGB76_15760"
     CDS             1231..2478
                     /locus_tag="VGB76_15760"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEY0100401.1"
                     /translation="MTERLYYHDSHLTQFDARVLGVFDHGAGRFAVTLDRTAFYPTGG
                     GQPCDTGTLGEARVVECVEEEETGIVRHVVEGAAPRVGARVEGRVDWSRRLDHLQQHT
                     GQHILSQAFVRLFDAQTRAFRMLERASEIDLDLREPSSERIEQAVELANRIIWEDRAV
                     SIRRVSSAEAAALPLRKDSAREGDLRIIEIENFDLSPCGGTHAARTGEVGILAVRSWE
                     RAKGLTRIEFLAGGRVLADYRRANHTARSVAALFSAGRDDAQELAARVLEENKLLSRR
                     LRTLEEIAARVEAEELIETATPVVGDDRGTRIVTRAFADRDAESLKRLALSIISRPGL
                     IALLGSRDADGRAARLVFARAPEAHGDMNALMREACALIEGRGGGRPDLAQGGGTNVE
                     KLTTTLETATTRLLAPQQPALPD"
     gene            2633..2708
                     /locus_tag="VGB76_15765"
     tRNA            2633..2708
                     /locus_tag="VGB76_15765"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2666..2668,aa:Ala,seq:cgc)
     gene            complement(2990..>3664)
                     /locus_tag="VGB76_15770"
     CDS             complement(2990..>3664)
                     /locus_tag="VGB76_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100402.1"
                     /translation="MRYQADSDLAMILPLPTPPSPSPDAVRFLDLSGYPQFFDDMNKG
                     FITYLTRSSATRARPADMPTLKVHEVGSFEASFVPHLRDFARLDSRFRLPDQVWEQMP
                     QYSDYAFAVFKLRAGAKNVHPMAFEFPRRNPQELFFPTVHVHHGIVNAYARFDHALYY
                     QTPQERISGMFSSVGLSSDLSDGPARQFMNIAKTQGIVDADALIQIRRLHGRRVNRDI
                     VVKEGI"
BASE COUNT          753 a         1118 c         1084 g          709 t
ORIGIN      
        1 ggtaggtctt gccgtcaact ttgaattcgt caatggtgaa gtcgggcgcg atctcggtgg
       61 gcgtgtcgat caggatgtcg tagttcggaa actgccactc gcgttgatcc gcgcgctccg
      121 tggcggagtt gatgatacgc caaccggcgg gcgcgtcgat ggagagacgc acggggtctg
      181 gcttgtggcc gacgacgtac atgaagaggg agccgccgtt gaagttcgcg tggcgcgcgt
      241 cgagttgcga aaacgtgccg gagagatcgt tggcgaagac tttataactg agcgcgacgc
      301 cctgcgcttt gccgggaaag acgcgccacg tctgcgtgtc gaggcgcgtg aggggaagcg
      361 tcgcggcatc gcagaaagca ggcggcgcgc aaaaagttgt cgcgcgcgcc tcctgcacat
      421 tcttcgcaaa atcgaagacg gcgtagcgtc ccggcgacca gcgcggcatc tgaaaatcga
      481 cggcctccac gaccagcggc gtctcaaccg tgatgcgcac ttcaaacagg tgcgaggccg
      541 ggttcggcat cgagagatgg taattgatct tgtattgttc gtcgcgcgac tgcgtagagt
      601 ttaacgcaga agtttgcgcg gaattcgact tggagtttgg cgcgaagttt tgcgcgcccg
      661 tcgtcgagcc aaacagagcg agcagggcga agaggagggc ggcgcgttgc cccgcgcgga
      721 gtttcatgca agcgttcata agtgctcccg aaaaagatgc gtcccgattt gaatggagtt
      781 ttgcagacga acgattatcc gcgctccggc gaacgcatgt caaaagaggg cggctggtgt
      841 gactacggag actccgggag tattagcgat attgtgaatc caccagcccg gtgatagcct
      901 ctgcggcatg aaacgccgct ggttccatct tatcgttgcg cttctgagcc tgcttgccat
      961 gttgctctta tcacgaatat taaatccgcc ctgcattccc ccgccgcctt gtaatcgagc
     1021 ggatgcggag gcgggtcgtt gcacaccgca accgttcctc tgcacagaag tataaaattt
     1081 ttcggacaca cagatttcaa actgaaccac tccctcaaag aggcacgcgc cgtgcgctcg
     1141 ccttgacgct ccgggcgcgg ctccgctagt ttcagccgcg cgagctttta tgtctacgac
     1201 ttcaactttc agccacccgc agagatgagc atgaccgaac gcctctatta ccacgactcg
     1261 catctcacgc agttcgacgc acgcgtcctc ggcgtcttcg accatggcgc gggacgcttc
     1321 gccgtcacgc tcgaccgcac cgcgttctac ccgacgggcg gcggccaacc gtgcgacacg
     1381 gggacgctcg gcgaggctcg cgtcgtcgaa tgtgtggagg aggaagagac gggtatcgtg
     1441 cggcacgtcg tagagggggc ggctccgcgt gtgggcgcgc gagtcgaagg acgggttgat
     1501 tggtcgcgcc gcctcgacca cttgcagcag cacacggggc agcacattct ctcgcaagcc
     1561 ttcgtccgac tcttcgacgc gcagacgcgc gccttccgca tgttggaacg cgcctccgag
     1621 attgacctcg atctccgcga gccttcaagc gaacgcatcg agcaggccgt cgaactcgcc
     1681 aaccgcatta tctgggaaga ccgcgccgtg agcattcgtc gagtctcttc ggcagaggcc
     1741 gccgcgctcc ccctgcgcaa agattccgcg cgcgagggcg acctgcgcat catcgagatt
     1801 gagaattttg atctgagtcc ctgcggcggc acgcacgccg cacgcacggg cgaagtcggc
     1861 atcctcgccg tgcgttcctg ggagcgtgcg aagggtctga cgcgcatcga gtttctcgcc
     1921 ggaggccgcg tgctcgcaga ctaccggcgc gccaaccaca cggcgcgctc cgtcgccgcc
     1981 ctgttcagcg cggggcgcga cgatgcgcag gagttagccg cacgcgtgtt ggaagagaac
     2041 aaacttctct cgcgccgtct ccgcacgctc gaagagatcg ctgcgcgcgt cgaagcggag
     2101 gaactgatcg aaacggccac acccgtcgtc ggcgacgaca gaggcacacg catcgtcaca
     2161 cgcgcctttg ccgaccgcga cgcggaatcc ctcaaacggc tcgcgctctc catcatctcg
     2221 cgccccggcc tcatcgccct gctcggctcg cgcgacgcgg acggtcgcgc cgcacgcctc
     2281 gtcttcgcac gcgcccccga agcgcacggc gacatgaacg cgctcatgcg cgaagcctgc
     2341 gccctcatcg aagggcgcgg cggcggacgc cccgacctcg cacagggcgg cggcacaaac
     2401 gtcgaaaaac taaccaccac actcgaaacc gccaccaccc gtctcctcgc ccctcaacaa
     2461 cccgcgctgc ccgactgaaa aacacggagg ctcccctcca cactgtcaaa agacgctgcc
     2521 ctccgcactg tcaaaagaca ccccgtgccc cgctcacgaa cgccgctcgc acgcccccgt
     2581 aacggacgct tgacaccgcc cccgccgctc tctaatattt ccctcgactc gtggggcagt
     2641 agctcagttg gcagagcgcc gcgttcgcaa tgcggaggcc aggagttcga ccctcctctg
     2701 ctccaccaaa agaaatcaac aagatacagt cgcctgcggg cggctgtttt tgtttcggga
     2761 cgctttcacg ggacgctttg cttcaataat tcacgccaaa gcattaggtt agaagtttga
     2821 gccgtctccg caccaagcat taacggcctg cctattcaag attgtatctg ctcatcgtta
     2881 tgcctgatac tctcaaccaa caaccagtaa ttcatcagac atttccatag attgagtagc
     2941 aatagttctt cacgaaaaaa aattccgcaa tgtccttgtc aggtcgtgct caaattcctt
     3001 ctttgacaac aatatctctg ttgacacgcc taccatggag gcgtcgtatt tgaattaggg
     3061 catcggcatc gacaataccc tgtgtcttgg caatgttcat gaattgcctg gccggtccgt
     3121 cgctaaggtc agatgatagg ccgactgaag agaacatccc actaattcgc tcttgcggtg
     3181 tctggtagta gagtgcgtgg tcaaaacggg cgtaagcgtt cacgatgcca tggtggacgt
     3241 gtacggtcgg gaagaataat tcctgcggat ttcttctggg aaactcaaac gccatggggt
     3301 gcacgttttt cgctccagcc cgcagtttga agacggcaaa agcgtagtcg ctatattgtg
     3361 gcatctgctc ccagacttga tcgggcagac ggaagcggct atcgagccga gcgaagtctc
     3421 gcagatgcgg gacaaacgac gcctcaaagc tgccaacctc gtgaactttg agcgttggca
     3481 tgtcagcagg ccgggcacgt gtggcggaac ttctcgtaag atacgtgatg aaacctttgt
     3541 tcatatcgtc gaagaactgc ggataaccgg agaggtctag gaatcggaca gcgtccgggg
     3601 atggggaggg cggggtcggc agagggagga tcatcgccaa gtctgagtcg gcttgatacc
     3661 gcat
//
LOCUS       DASWNS010000458         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_302470, whole genome shotgun sequence.
ACCESSION   DASWNS010000458 DASWNS010000000
VERSION     DASWNS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302470"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1755
                     /locus_tag="VGB76_15775"
     CDS             61..1755
                     /locus_tag="VGB76_15775"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY0100403.1"
                     /translation="MTENTNTPFIEDNDQTRARREHLARIQALTGNAYPNKFRRSNVS
                     NNQTEGEDTITSVVGCEAITRHVPQLDAGARPTPEQLTAANEALKAFGDVVRVSGRLS
                     VPPRVMGKAAFVHLSDGKERLQIYVRRDDVQTMNYAGRTLDGWELFSLLDHGDFIGVE
                     GFLFVTKTGELSIHVRSFEFLAKALLPMPDKLHGISDPEIRQRQRYADLIAGSLKIDR
                     DDAPTEGSVPRELTTREVFERRSKIIREMRTFLDERGYIEVETPMLTPIASGAAARPF
                     KTHHNALDIDLYARIAPELYLKRLLVGGFERVYELNRNFRNEGISTRHNPEFTMLEFY
                     CAYMDVNGMMDFCEELLRRSVLAATGGTTVRFEDHEFDFGRPFERVSMRDVVKRFAPD
                     VELTDGNLVDMFETHAEPHLMQPTFIIDFPKAISPLSKTSPDNPAIAERFELFINAME
                     CANGFSELNDPLEQYERFREQGQARERGDEEAMQMDTDYVRALAYGMPPAAGIGIGID
                     RLVMLLTNRRSIRDVILFPHMRPAAPKTEETDAPPALPIEQAGEQTKAAKEGADGQ"
     gene            complement(1882..>3362)
                     /locus_tag="VGB76_15780"
     CDS             complement(1882..>3362)
                     /locus_tag="VGB76_15780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100404.1"
                     /translation="ARRAVAPSAGAPPVAPAAAKPSVAPSSAVAAPSVAPPVVVAAPV
                     EKSPAATASASKSEAAASHSKGADGELERLRAEIKDTKAGAERTRLQRALVERLVELK
                     REPEALDALRLMIHEDRYDPPFFFNTGNALARLGDASAAEDAYRKAILQRRGNYSRAL
                     NNLGVILIRQGHWDEAQETFAAALLQENHAYPEASYNLGRLYLLRGEADLAIREWTRT
                     LRLEPAHAEAAAALARAYAEDGDTKRGLAVLDNFVARSTRGGTSGVPREISEARRDLV
                     AAAAAHANEDDATNAKTETSEGAGGNRAGRASSNANFRALEVDRASYDLLRAARTQRE
                     RGSYEESVKVYRDVLARHPAGYFPPANLELGAALINLRRDTEAITALVPLTQKGGARF
                     PVAYYHLGRLYERQGELARAVQHFARAAELYGDANPQVLIDLSRTREKTNDPAGALAA
                     MDAYAASIARQGTVPAWVAERQAKLKQKLSTTQATQTSTAKP"
BASE COUNT          612 a         1056 c         1071 g          623 t
ORIGIN      
        1 cggctactga caacccgcca cttcttgcta tactccgctt ttctcttgga gcaggaatag
       61 atgacagaga acaccaacac gcccttcatc gaggacaacg atcagacgcg cgcacgccgc
      121 gagcacctcg cacgcattca ggctctcaca ggcaacgcct acccgaacaa gtttcgccgc
      181 agcaatgtct ccaacaatca gaccgagggc gaagacacga ttacgtccgt cgtcgggtgc
      241 gaagcgatca cgcgtcacgt cccgcagttg gacgcgggcg cgcgcccgac gccggaacaa
      301 ctcacggcag ccaacgaggc gttgaaggcg ttcggcgacg ttgtgcgcgt gagcggacgc
      361 ctcagcgtgc cgccgcgcgt gatgggcaag gccgccttcg tgcatctctc cgacggcaaa
      421 gaacgcttgc agatttacgt gcgccgcgac gacgtgcaga cgatgaacta cgccgggcgc
      481 acgctcgacg gatgggaact cttctcgctg ctcgatcacg gcgacttcat cggcgtcgaa
      541 ggtttcctct tcgtcacgaa gacgggcgaa ctctccatcc acgtccgctc gttcgagttt
      601 ctcgccaaag ccctgctccc aatgcccgac aagctgcacg gcatcagcga ccccgaaatc
      661 cgccaacgcc aacgctacgc agacctcatc gcgggcagcc tcaagatcga cagggacgac
      721 gcgccgacgg aaggctccgt gccgcgcgaa ctcaccaccc gcgaagtctt cgaacgccgc
      781 tcgaagatca tccgcgagat gcgcaccttt ctggacgagc gcggctacat cgaagtcgag
      841 acgccgatgc tcacgcccat cgcctccggc gcggcggcgc gtcccttcaa gacgcaccac
      901 aacgccctcg acatagacct ctacgcgcgc atcgcccccg aactctacct caagcggctc
      961 ctcgtcggcg gcttcgaacg cgtctacgaa ctcaaccgca acttccgcaa cgaaggcatc
     1021 tccacccgcc acaaccccga attcaccatg cttgaattct actgcgccta catggacgta
     1081 aacggcatga tggacttctg cgaagagttg ctgcgccgga gcgtgctcgc agcaacgggc
     1141 gggacaacag ttcgctttga agatcacgag ttcgatttcg gtcgcccttt cgagcgtgtg
     1201 tctatgcgcg acgtggtgaa gcgtttcgct cctgacgttg agttaaccga tggaaatttg
     1261 gtcgatatgt tcgagactca cgcagagccg catctgatgc agccgacttt cattattgat
     1321 tttcccaaag cgatttcgcc cctctcgaaa acgtcgccgg ataatccggc aatcgcggag
     1381 cggttcgagt tgttcattaa cgcgatggag tgtgcgaacg ggttttcgga gttgaatgat
     1441 ccgttggagc agtatgagcg gtttcgtgag caggggcagg cgcgggagcg tggggatgag
     1501 gaggcgatgc agatggatac ggattatgtg cgcgcgctcg cctacgggat gcctccggca
     1561 gcagggatcg gcatcggcat tgaccgcctc gtgatgctgt tgaccaaccg gcgttccatc
     1621 cgcgacgtga tcctcttccc gcacatgcgc cccgccgcgc cgaaaacgga agagacggac
     1681 gcgccgcccg cattacctat cgaacaggcg ggcgagcaaa cgaaagcagc taaagaaggg
     1741 gcggacgggc agtagttttt attgccgccg cgcgagtagc gaaagataaa agcccacggt
     1801 ttgcgccgtg ggcttttatc tttcaacgct cgcgcggttg cgagttgtga cacggggagt
     1861 tcgcgtctgc cggtcgtggc tttacggttt cgccgtcgaa gtttgcgtcg cttgggtcgt
     1921 ggagagcttc tgcttgagct tcgcctgtcg ctccgcgacc cacgcgggga ctgtgccctg
     1981 tcttgcgatt gaggcggcgt aggcgtccat ggcggcgagc gcgcctgccg ggtcgttcgt
     2041 cttctcgcgg gtgcggctga ggtcaatgag cacttgcggg ttagcgtcgc cgtagagttc
     2101 ggcagcgcgt gcgaagtgct gcacggcgcg tgcgagttcg ccctgacgtt cgtagagacg
     2161 accgagatga taataggcga cgggaaaacg cgcgccccct ttttgcgtga gcgggacgag
     2221 cgcggtgatg gcctccgtat cgcgcctcag attgatgagc gcggctccga gttcgagatt
     2281 ggcgggcggg aaatatcctg ccgggtggcg tgccagaaca tcccgataaa ccttcacaga
     2341 ctcttcataa cttccccgtt cgcgttgcgt ccgcgccgct cgcagcagat cgtaagaggc
     2401 gcggtcaact tcgagggcgc gaaagttggc gtttgaagaa gcacgcccgg cacggttgcc
     2461 gcccgcgcct tcgctcgtct cagtcttcgc gttcgtcgcg tcgtcttcgt tcgcgtgagc
     2521 cgcagcggcg gcgacgagat cgcggcgcgc ttcactgatc tcacgcggga cgcccgacgt
     2581 gccgccgcgc gtcgagcgtg caacgaaatt atcgagcacc gcgagtccgc gcttcgtgtc
     2641 gccgtcttcc gcgtaggcgc gcgcgagggc ggcggcggct tccgcgtgcg cgggttcgag
     2701 gcgcagcgtg cgcgtccatt cgcggatggc gagatcggct tcgccgcgca gcaggtagag
     2761 ccgaccgagg ttgtagctcg cttccgggta agcgtgattt tcctgcaaga gcgcggcggc
     2821 gaaagtctcc tgcgcttcgt cccaatggcc ttgccggatg agtatgacgc cgaggttgtt
     2881 gagggcgcgc gagtagttgc cgcgacgctg gaggatggct ttgcgataag catcttccgc
     2941 cgcgctcgcg tcgccgagac gcgccagcgc gtttccggtg ttgaagaaga agggcggatc
     3001 ataacggtct tcgtgaatca tcaggcgcag ggcatcgagg gcttccggct cgcgcttgag
     3061 ttcgacgagt cgttcgacga gcgcgcgttg cagccgcgtg cgctccgcac ccgccttcgt
     3121 atctttgatc tctgcgcgca ggcgttcgag ttccccgtct gctcctttcg agtgagacgc
     3181 cgccgcctcg ctcttcgacg cggaagctgt agcagcgggc gacttctcga ccggcgcagc
     3241 gacgacaaca ggcggcgcga cggacggcgc agcgacagcc gacgacggcg caacggacgg
     3301 cttagcagca gcgggcgcga cgggcggagc acccgccgaa ggcgcgacgg cgcgacgggc
     3361 gc
//
LOCUS       DASWNS010000459         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_510348, whole genome shotgun sequence.
ACCESSION   DASWNS010000459 DASWNS010000000
VERSION     DASWNS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510348"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..2421
                     /gene="feoB"
                     /locus_tag="VGB76_15785"
     CDS             109..2421
                     /gene="feoB"
                     /locus_tag="VGB76_15785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEY0100405.1"
                     /translation="MSTTLQSPAPVQEPVATLEADDVRPLTIALAGNPNAGKTSLFNV
                     LTGLRQKVANYPGVTVERKEGLWQLSPDLPTAQLIDLPGLYSLDANSIDEQIARDVIT
                     GRLTSVPLPDCIIVVVDATNLERNLYLATQLLETERPLVVALTMIDLAARRKLEIDTA
                     RLSGALGVPVVPVVATQRQGLDDLAAAVVEAAKSQPPAARWQLSTEAERELSALAAAF
                     DGNGAHAGVDEENSARRYSALVELYAEELPEGATEERQAAITSARERLAAGNPRWWQE
                     PLLARYEWIERVARSTVRDHKPGRTSRTDRIDRFVTHRFFGPLILLFVMLVVFQTIFS
                     WAQLPMDLIDGSFGKLGELVRASMPPGLLTDLLVDGVIAGVGGVLVFLPQILLLFFFV
                     SLLEDSGYMARAAFLMDRLMRGVGLHGKAFMPLLSSFACAIPGIMATRTIENPKDRLA
                     TILIAPFMSCSARLPVYTLMIAAFFTGQRKIFGVLSVGVLIIMAMYLLGIAVAVVVAW
                     ILKHTILKSPPPPLVLELPPYRTPSFFNVLQTIYARSLMFVRRAGTVILAISILLWAL
                     VTFPRMEDGGNVPNADATTAATTAASPNRAPDERATTAQREETVQPAAGASNVAEAVD
                     GDEGEQIRQSYAGRLGHFIEPAIAPLGFDWKMGIGLIASFAARETLVSTLSIVYNVGA
                     DATEESDTLIGAVRNARRADGTPAWTPLVAVSMMVFFVLACQCMSTIAIVRRETNSWR
                     WPLFMVSYMLVLAYVASFITYQGGQLLGFS"
     gene            2426..2683
                     /locus_tag="VGB76_15790"
     CDS             2426..2683
                     /locus_tag="VGB76_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEY0100406.1"
                     /translation="MFSDWQTIAVWLIIFAAFAYVGRRGWGRLRSFGASGRAQGASCA
                     TGCGSCGDEQTTKAHSATAKPQNVLVQITNAGRETRRQHKP"
     gene            complement(2835..3683)
                     /locus_tag="VGB76_15795"
     CDS             complement(2835..3683)
                     /locus_tag="VGB76_15795"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HEY0100407.1"
                     /translation="MTRSPLDQPYRGDGSDRPVGVPFPPERLPLLRARQLRKRWQYVS
                     FWSGELSFCAARVRVGTLLQEYWGIWDRAAGQFRERTHLFTSRVEFAPNRVRMRDGDA
                     EIDVAYEPHSSFEVYRPANRAYIWSHKDYCRAARGVVRYGGTTREAVGVVFVDFNAGY
                     HERRTHWRWAAGAGLDQHGRIVGFNAINGLFDTPAQSERTIWIDGAAHETGPVTFSED
                     LSTVSFAEGGTLRFHPEALIENHDNFLLVRSDYYHWFGVYTGTLPGGIELREAYGVRE
                     RQDALW"
     gene            complement(3900..>4317)
                     /locus_tag="VGB76_15800"
     CDS             complement(3900..>4317)
                     /locus_tag="VGB76_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100408.1"
                     /translation="VMNADGSNKLPLTERHSNTNSMQADRVPAWSPDNSRIIFARSLS
                     IFFANESGAEDANALYSMNPDGSGLALFDDADPSTNTEPAWSPDAAKVVYASGYDPWS
                     ESPNRDIYVSNARGAKQTVQLTNHSADDTKPAWQSR"
BASE COUNT          750 a         1373 c         1357 g          837 t
ORIGIN      
        1 cgtgaatcgt caatagaaaa acggattgaa tctattcacg attgacgatt cacgatttac
       61 ttgccttcaa ctcgtcacct gtcacttgtc acccgtcact gttttcttat gagtacgacg
      121 ctgcaatccc ccgcccctgt gcaagagccg gtcgcgacgc ttgaggctga cgacgtgagg
      181 ccgctgacga ttgcgctcgc gggcaatccg aacgcgggca agacgagcct cttcaacgtg
      241 ctcacgggac tccggcagaa ggtcgccaac tatcccggcg tcacggtgga acgcaaagag
      301 ggactctggc agctctcgcc cgatctgccg acggcgcaac tcatagacct ccccggcctc
      361 tacagtctcg acgccaactc cattgacgaa cagatcgcgc gcgacgtgat caccggacgc
      421 ctcacgagcg tgcccctccc ggactgcatc atcgtcgtgg ttgacgccac gaacctcgaa
      481 cgcaatctct atctcgccac gcaactctta gagactgaac gcccgctcgt cgtcgccctg
      541 acgatgatcg atctcgccgc gcggcgcaaa cttgagatcg acacggcgcg attgtccggt
      601 gcgctcggcg ttccggtcgt gcccgtcgtc gccacgcagc ggcagggact cgacgatctc
      661 gccgcagccg tcgtggaggc cgccaagtct cagccgcccg ccgcccgctg gcaactctcc
      721 acggaggccg aacgcgaact ctccgcgctc gccgccgctt tcgatggcaa cggcgcacac
      781 gccggcgtag atgaagagaa cagcgcgcga cgttacagcg cgctcgtcga actctacgcg
      841 gaagaattac cggagggggc gaccgaggag cgtcaggcgg cgatcacgtc ggcgcgcgag
      901 cggctggccg caggtaatcc gcgctggtgg caagagcctc tgctcgcgcg ttacgagtgg
      961 atcgaacgcg tcgcgcgcag cacggtgcgc gatcacaagc cggggcgcac ctcccgcacg
     1021 gatcgcattg atcgtttcgt cacacacaga tttttcggtc ccctgatcct gctcttcgtg
     1081 atgctcgtcg tcttccagac gatcttttcg tgggcgcaac tgccgatgga tttgatcgac
     1141 ggcagcttcg gcaaactcgg cgaactcgtg cgcgcttcga tgccgcccgg cttgctgacg
     1201 gacttgctgg ttgacggcgt gatcgcgggc gtcggcggcg tgctcgtctt tctgccgcag
     1261 attctgttgc tcttcttctt cgtctcactg ctggaagaca gcggctacat ggcgcgcgcg
     1321 gcctttctga tggacaggtt gatgcgcggc gtcgggcttc acggcaaggc tttcatgccg
     1381 ctgcttagca gcttcgcgtg cgccattccg ggcatcatgg cgacgcggac gattgagaac
     1441 ccgaaggatc gtctggcgac gatcctgatc gcgccgttta tgagttgctc ggcgcgactg
     1501 ccggtttaca ctctgatgat cgcggccttc ttcacggggc aacgaaagat tttcggcgtg
     1561 ctctccgtcg gcgtgctgat catcatggcg atgtacctgt tggggatcgc ggtggcggtc
     1621 gtggtcgcat ggattttgaa gcacacgatc ttgaaatcgc cgccgccccc gctcgtgctg
     1681 gaactaccgc cctaccgcac gccgagtttc ttcaacgtcc tgcaaacgat ctacgcacgc
     1741 tccctgatgt tcgtgcggcg tgcgggcacg gtcatcctcg ccatctcgat cctgctctgg
     1801 gcgctcgtca ccttcccgcg catggaggat ggcggcaatg tcccgaacgc cgatgcgacg
     1861 acggctgcga cgacggcagc gtcgccgaac cgcgcgccgg atgagcgtgc gacgacggcg
     1921 caacgcgagg agacggtgca accagccgcc ggagcatcga atgtggccga agcggtggac
     1981 ggcgacgagg gcgagcagat tcgacagagt tacgcggggc ggctcggcca cttcatcgaa
     2041 ccggccatcg cgccgctcgg cttcgactgg aagatgggca tcggcctcat cgcctcgttc
     2101 gccgcgcggg aaacgctcgt ctcgactttg agcatcgtct ataacgtcgg cgcggatgcg
     2161 acggaagagt cagacacttt gatcggcgcg gtgagaaacg cgcggcgcgc ggacggcact
     2221 ccggcatgga cgccgctcgt cgccgtctcg atgatggtct ttttcgtgct ggcctgccag
     2281 tgcatgtcaa ccatcgccat cgtgcggcgc gagacgaatt cgtggcgttg gcctctgttc
     2341 atggtgagct acatgctcgt gctcgcttac gtcgcatcgt tcatcacgta tcagggcggg
     2401 cagcttttgg gattcagtta agcctatgtt ttcggattgg cagaccatcg ccgtgtggct
     2461 catcatcttc gccgccttcg cctacgtcgg gcggcgcggg tggggacgcc tgcgttcgtt
     2521 cggcgcgagc gggcgtgcgc agggtgcgtc gtgcgcaacc ggctgcggca gttgcggcga
     2581 tgaacagaca acaaaggcgc actccgcgac ggcaaagccc caaaatgtac tcgtccagat
     2641 caccaacgcg gggcgcgaga cgcgacgaca gcataaaccg taggcgtcgg gaatgtgtga
     2701 cgtgaatgaa aatgccggcg cagtctcagt gagattgcgc cggctttctc gttgcgcggt
     2761 cgcttcgccc gcgctgcgcg ggagtcagct aatgaaatga tcgccccggc gtgcgggcgc
     2821 gctcgtccgc gtcgttacca gagcgcgtct tgacgttcgc gtacgccgta ggcttcgcgc
     2881 agttcgatgc cgccgggcag cgtgcccgtg taaacgccga accaatggta atagtccgaa
     2941 cgcacgagca gaaagttatc atggttctcg atcaacgcct ccggatggaa acgcagcgtg
     3001 ccgccttcgg cgaaagagac ggtcgagagg tcttccgaga aggtgacagg ccctgtctcg
     3061 tgcgccgcgc cgtcaatcca gatcgtgcgc tcgctctgcg cgggcgtgtc gaacaggccg
     3121 ttgatggcgt tgaagccgac gatgcgcccg tgctggtcca gccccgcacc ggctgcccaa
     3181 cgccagtgcg tgcgccgctc gtgataaccc gcgttgaagt ccacgaagac gacgccgacg
     3241 gcctcgcgcg tcgtgccgcc gtagcgcacg acgccccgcg ccgcgcggca gtagtccttg
     3301 tgcgaccaga tgtaagcccg gttggccggg cggtagactt cgaagctgct gtgcggctcg
     3361 taagccacgt cgatctcggc gtcgccgtcg cgcatgcgga cgcggttcgg cgcaaattcg
     3421 acgcggctcg tgaagaggtg cgtgcgctcc cgaaactgtc ccgccgcgcg atcccagatg
     3481 ccccagtact cctgtagcaa cgtccccacc ctgacacgcg ccgcgcagaa agataactcc
     3541 ccgctccaaa aggagacgta ctgccagcgt ttgcgcaact gccgcgcgcg cagcaacggc
     3601 agacgctcgg gcgggaacgg cacgccgacg ggacggtcgc tcccgtcgcc gcgataaggt
     3661 tgatcgagag gtgatcttgt catccggcga ttatactcga ttccgctagg gggcgagttt
     3721 tcccgacatg cacgcacgca gacgccccgc tcgcctcgcc cgcgacaaag gcagagcggc
     3781 ctgacaagaa gttttttttg aactctcgtc aggccgcttc gcgttaaaag tggaagcgac
     3841 cgggtctgtt ctgggtcgct tccgccgtga gtttacgacc tgtgtaatta acgcgacggc
     3901 taacgagatt gccacgcggg cttcgtgtca tccgcgctgt gattcgtcag ttgcacggtc
     3961 tgttttgcgc cgcgcgcgtt cgagacgtag atgtcgcggt tggggctttc gctccaagga
     4021 tcgtagccgc ttgcgtacac aaccttcgcg gcgtcgggcg accacgcggg ttccgtgttg
     4081 gtcgaaggat cagcgtcgtc aaacaacgcc aagccgctgc catccgggtt catgctgtag
     4141 agcgcgttgg catcctcggc tccgctctcg ttggcaaaaa agatcgacag gctgcgcgca
     4201 aagatgattc tggaattgtc gggcgaccac gccgggacac ggtcggcctg catcgagttc
     4261 gtattactgt gcctctctgt cagaggcagt ttgttgctgc cgtcggcatt catcacg
//
LOCUS       DASWNS010000460         4094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_302483, whole genome shotgun sequence.
ACCESSION   DASWNS010000460 DASWNS010000000
VERSION     DASWNS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302483"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..2437
                     /gene="recG"
                     /locus_tag="VGB76_15805"
     CDS             29..2437
                     /gene="recG"
                     /locus_tag="VGB76_15805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY0100409.1"
                     /translation="MSLTLDTPILSLTGHGIPRFGQPTARRLGLALASLYGKREAGEA
                     TVEDLLNYLPMRYEDRSNLTRIEELRDGVEASLELYVRVAGGFQVGKNRGAKAPPLYI
                     FEITASDPERTAKPVVVFWFVSGRQATRIVAYHRQRFTRGARFVAYGKWEWDARRNTF
                     ALRLNKPDELEMLPGIWTPPEHALLRLAEENGDHAGEGSHTQRANGGEATQPSDESES
                     DPENEDSGGDLDTLAETDEAEVSDPRLAAIHVGRRVPVYRKLGEFPAKRMREIMHAVL
                     ARIDDAEITETLPADLIGRQKLISRAEALRRIHFPTEDAPLADYERARSPAHIRLIFE
                     EFFWVAIAIAIRKSERVKEPKGAVIEIDERVRTHLSEILPFTLTSAQQRSITRILDDM
                     QSDVPMNRLLQGDVGSGKTIVALLAMLAAMENGYQAALMAPTEILADQHARNIKRMLA
                     RTPHRAELLIGSLRAAEKRRLHADIAAGEVNACIGTHALIQEAVAFRKLGIVIIDEQH
                     RFGVLQRAALRERGFNPDVLVMTATPIPRSLAMTVYGDLDVSVIDELPPGRTPIKTVV
                     VGEDQRAGVYKGVEREIRAGRQAYIVYPLVEESEKMDLKDATRMFEHLRDRVFPTFQV
                     GLLHGKMKSAEKEETMHRFTRGEIQILVATTVVEVGVDVPNASVMVIEHAERFGLSQL
                     HQLRGRVGRGAEQSFCVLLASDKQTAVAKERLGIMEETNDGFRIAEKDLEIRGPGEVM
                     GTRQSGVPTFRVGNLVRDVRILEEARNEADYYLSNARRRTRETSRLIERVRADVRFGL
                     ATVG"
     gene            2678..3247
                     /gene="rsmD"
                     /locus_tag="VGB76_15810"
     CDS             2678..3247
                     /gene="rsmD"
                     /locus_tag="VGB76_15810"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY0100410.1"
                     /translation="MRIIAGQYRGRVLKSPPSMQIRPTSDRLRETLFNVLAPRIEGAR
                     FLDLCAGSGAVGIEAISRGASFVTFVDRSRKMCGLVEANLDLCGVPEESTAVVLSEVA
                     DYLRRAETWGGDPYDIAFLDPPYAIDYAPLLDLFGTRTPALLTAGGILIVEHHHKTNL
                     KDAVGNIRRWRILKQGDSALSFYEVEGAE"
     gene            3309..>4094
                     /gene="tsaD"
                     /locus_tag="VGB76_15815"
     CDS             3309..>4094
                     /gene="tsaD"
                     /locus_tag="VGB76_15815"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY0100411.1"
                     /translation="MLVLGIESSCDETAAAIVRDGREMISSVISSQIETHRRFGGVVP
                     ELASREHLDKIVPIVEEAFARARVEKREIDGIAVTQGPGLVGSLLVGLSYAKAMAYAL
                     EKPLVGVNHIEGHIYSVVFENPPILYPAFALIVSGGHTNLFLVPAPGQYKVLARTRDD
                     AAGEAFDKVAKMLGLGYPGGPIIEGLAREGDPQAVRFSVPRMGDGLPDFSFSGLKTAV
                     SKYVRETGLQPVAEGEAASQAVKDLAASFQSVVVRSLVGTTERL"
BASE COUNT          804 a         1340 c         1206 g          744 t
ORIGIN      
        1 tcgccacttg tcacttgtcg ctgcttttat gtctcttacg ctcgacactc cgattctctc
       61 actcacaggg cacggcatcc cgcgtttcgg ccagccgacg gcgcgtcgtc tcggcctcgc
      121 gctcgcgtct ctctacggca aacgcgaggc gggcgaagcg acggttgaag acctcttaaa
      181 ctacctgccg atgcgctacg aagatcgctc gaatctaacg cgcatcgaag aactccgcga
      241 tggcgtcgaa gcgtcgctcg aactctacgt gcgcgtggcg ggcggctttc aggtcggcaa
      301 aaatcgtggg gcgaaagcgc cgccgctcta catcttcgag atcactgcga gcgacccgga
      361 gcgcacggcg aaacccgttg tcgtcttctg gttcgtgtca ggccgccagg ccacgcgcat
      421 tgtggcttat catcgccaac gcttcacgcg cggcgcacgc ttcgtcgcct acggcaagtg
      481 ggaatgggac gcgcgccgca acaccttcgc cctgcgtctg aataagcccg acgaactcga
      541 aatgctcccc ggcatctgga cgccgccgga acacgccctg ctccggctcg ccgaagagaa
      601 cggcgatcat gcgggcgaag gctcacacac tcaacgcgcc aacggaggcg aagcgactca
      661 acccagcgac gaaagcgaga gcgacccgga gaacgaagat tcaggcggcg acctggatac
      721 gctggcggag acggacgagg cagaggtcag cgatccgcgc ctggcggcga ttcacgtcgg
      781 gcgacgcgtg ccggtctatc gcaagctcgg cgagtttccc gcgaaacgca tgcgcgagat
      841 catgcacgcc gtcctcgcac gcatcgacga tgcggagatc acagagacgc tgcccgccga
      901 tctcatcgga cgacaaaagt tgatctcccg cgccgaagcc ctgcgtcgca tccactttcc
      961 gacagaagac gccccgctcg ccgattacga acgcgcccgc tcgcccgcac acatccgtct
     1021 catcttcgaa gagttcttct gggtcgccat cgccatcgcc atacgcaaaa gcgagcgcgt
     1081 caaagaaccg aaaggggcgg tcatcgaaat tgacgaacgc gtgcgcacgc acctctcgga
     1141 gatacttccc ttcacgctga cgagcgcgca gcaacgttcc atcacgcgca tcctcgacga
     1201 catgcaatcc gacgtgccga tgaaccgcct gttgcaaggc gacgtgggca gcggcaaaac
     1261 catcgtcgcc ttgctcgcca tgctcgccgc gatggagaac ggttatcagg ccgcgctcat
     1321 ggctccgacc gaaatcctcg cagaccagca cgcgcgcaac atcaagcgca tgctcgcgcg
     1381 cacgccgcac cgcgccgaac tcctcatcgg ctcgctccgc gccgccgaga aaaggcgtct
     1441 gcacgcagac atcgccgccg gagaagtcaa cgcctgcatc ggcacccacg cgctcattca
     1501 agaggcggtc gccttccgca aactcggcat cgtcatcatc gacgagcagc atcgcttcgg
     1561 cgtccttcaa cgcgcggcct tacgcgaacg cggcttcaac ccggacgtgc tcgtgatgac
     1621 ggcgaccccc atcccgcgct cgctcgccat gaccgtctac ggcgacctcg atgtctccgt
     1681 catagacgaa ctcccgcccg gacgcacgcc gatcaaaacg gtcgtcgtcg gcgaagacca
     1741 gcgcgccggg gtttataaag gagtcgaacg ggagatccgc gccgggcgtc aggcttacat
     1801 cgtctacccg ctcgtcgaag aatcggagaa gatggacttg aaagacgcga cgcgcatgtt
     1861 cgagcatttg cgcgaccgcg tgttccccac cttccaggtc ggcctgctgc acggcaagat
     1921 gaagtctgcg gagaaagagg agacgatgca ccgcttcacg cgcggggaga ttcaaattct
     1981 cgtcgccacc accgtcgtcg aagtcggcgt tgacgtgccg aacgcgtccg tcatggtcat
     2041 cgaacatgcg gagcgtttcg gcctctcgca gttacaccag ttgcgcgggc gcgtggggcg
     2101 cggcgcggag cagagtttct gtgtgctcct cgcctccgac aaacaaacag ccgtcgccaa
     2161 agaacgcctc ggcatcatgg aagagacgaa cgacggtttc cgcatcgccg aaaaagatct
     2221 ggagatacgc ggacccggcg aagtgatggg cacgcgccaa tccggcgtcc cgaccttccg
     2281 cgtcggcaac ctcgtccgcg acgtgcgcat acttgaagaa gcgcgcaacg aagccgacta
     2341 ctatctctcc aacgcgcgcc gccgcacgcg cgaaacctcc cgcctcatcg aacgagtccg
     2401 cgccgacgtg cgcttcggtc tggcaaccgt cggctaaaca gtgatacgct agccctcccc
     2461 gctcacatcc acacaccaaa cagccccgtc ggaacctgta tcgccgtcga gccgcgccat
     2521 cgttttcctt gacccctgac gctggaggtt tatgagaata aagaatcagg tgtttgaggt
     2581 cgcagggctt cctgttaatc ctgctcgctt ctgacttctg aattctgact cctgagttct
     2641 gaattctcat ccgcctctca tgtgctaaca ttccggcatg aggatcatcg cgggacaata
     2701 tcgcgggcgt gtgcttaaga gtccgccgtc gatgcagatt cgcccgacct ccgaccgcct
     2761 gcgcgagacg ctcttcaacg tcctcgcgcc gcgcatcgag ggcgcacgct ttctcgattt
     2821 gtgcgcgggt tcgggcgcag tcggcatcga agcgatctca cgcggcgcgt ctttcgtcac
     2881 cttcgttgac cgttcgcgta agatgtgcgg actcgtcgaa gcgaatctcg atctgtgcgg
     2941 cgtgcccgaa gagtcgacgg cggttgtcct gagtgaagtt gccgactatc tccgccgcgc
     3001 ggaaacatgg ggcggcgacc cctacgacat cgcttttctc gatccgccct acgccataga
     3061 ctatgcgccc ctgctcgatc tcttcggcac acgcaccccg gcgctgctca ccgcaggcgg
     3121 cattctcatc gtggagcatc atcataagac caaccttaaa gatgcggtcg gaaacatacg
     3181 ccgctggcgc atcctgaagc agggtgactc cgcgctcagt ttctacgaag tggaaggcgc
     3241 ggaataactc agaactcagt agtgatcccg ctccgtgggt gagactgctc ttcaactaat
     3301 tcttcaatat gcttgtacta ggaatcgaaa gttcatgcga tgagacggcg gcggccatcg
     3361 tgcgcgacgg gcgcgaaatg atctcgtccg tcatttcatc gcaaatcgaa acgcacagac
     3421 gcttcggcgg cgtcgtcccc gaactcgctt cgcgcgagca tctcgataag atcgtgccca
     3481 tcgtggaaga ggctttcgcg cgtgcgcgcg tcgagaagcg ggagattgac ggcatcgccg
     3541 ttacgcaggg gccgggtctg gtcgggtcgc tgcttgtcgg cctctcctac gcgaaggcga
     3601 tggcttacgc gctcgaaaag ccgctcgtcg gggttaacca catcgagggg cacatctact
     3661 cggttgtctt cgagaacccg ccgattctat atccggcctt cgcgctcatc gtgtcgggcg
     3721 ggcacacgaa tctttttctg gtgcccgcgc cgggacagta caaggtgctt gcgcgcacgc
     3781 gcgacgatgc ggcgggcgaa gccttcgaca aggtggcgaa gatgctcggc ctcggctatc
     3841 cgggcggtcc tatcatcgaa gggttggcgc gcgaaggcga tccgcaggcc gtccgtttca
     3901 gcgtgccgcg catgggcgac ggattgcctg attttagttt cagcggactc aaaaccgccg
     3961 tttcgaagta tgtgcgcgag acggggctgc aacccgtcgc ggaaggtgag gccgcttcgc
     4021 aggcggtgaa ggatttggcc gcgagctttc aaagcgtagt ggtgcgctcg ctcgtgggca
     4081 cgacggagag actg
//
LOCUS       DASWNS010000461         6352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_327331, whole genome shotgun sequence.
ACCESSION   DASWNS010000461 DASWNS010000000
VERSION     DASWNS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327331"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3219)
                     /locus_tag="VGB76_15820"
     CDS             complement(<1..3219)
                     /locus_tag="VGB76_15820"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEY0100412.1"
                     /translation="MQPAPSRKYLYTRARRTSFIAFTFGLLALFMLPDRFLKAFEPEV
                     QAVQAATTFNVNSTGDSPDSDTTDGVCNDGAGNCTLRAAIMQANATTGRDTINFAISS
                     GLQTIMPTAQLPVITDPLVIDATTQPGFAGQPVIELNCSGFSANPNSAALQITAGNSV
                     VRGLVINGFSDGGINLQTAGGNLVEGNYIGTNAAGTSSTNSRGNGISVSSSDNLIGGT
                     TPAARNVISATDSHGISIFNATTNNTVQGNYIGTNAAGTAELSNTHSGVFISAPNNTI
                     GGTVAGARNVISGNNVGITIQDASSNGNAIQGNYIGTDAAGSRDLGNLTGIAVFNASG
                     TVIGGTVAGARNVVSGNNFGIQMQGTSDSAVQGNYIGTTAAGSAPLGNAVFGLSISGN
                     VNALIGGTTAGAGNVVASTTGGAANTGNAIQILSSTNTKVQGNYIGTNAAGAGLGNSM
                     HGVSILGDSTSVNSIVGGSEPGAANVIAFNAGTGVLVNTTGTGHTVRANSIFSNGQLG
                     IDLGAAGVTPNDAGDPDTGSNNLQNFPLVRSVSTGGGSTNAQGTLNSAPNASYIIEVF
                     ANNSCDASGQGEGQFLLGLTTVNTDASGNAAFNATFPVQLLPGQSVTATATDASGNTS
                     EFSVCHEVDQPGHVQFSASSYTTAETSGSVVITVVRTFGAAETASVNYVTSNGSATAN
                     ADYTPASGTLFFAPGESMKSFTIQILDDSLGETTETVNLFLSNATGSTLGNPIYAVLN
                     ITDDDPLPEVSINNVTINEETANSGANAIFTIRLSEPSSNTVTVRYFTDDDTAQANAD
                     YTPLNLQITFTPGQTTKAIAVPIIADTIDEQNERFVVILTNAVNATISNPVGSGTIID
                     NDPDPGTTGELLITEFRLSAPGGANDEYIEIYNNTNSNIDLRNYSFVFLATSAGSPTT
                     LSFATLQNGFLLTPRSHRLIVNSTGYSLSAYAAGNLFTTGADFFSDNQGIGLRRNGVN
                     AHVDAVGFADDPAEFREGAGLPVISAFPALRPQYSYVRKSASGTPQDTGDNAQDFVLV
                     TTTGEMIAGVPSVLGAPGPQGQTSPVQRNAAIKPSLVDPVAAP"
     gene            complement(3443..4111)
                     /locus_tag="VGB76_15825"
     CDS             complement(3443..4111)
                     /locus_tag="VGB76_15825"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY0100413.1"
                     /translation="MKRKSKTIEREKDGFLYDADEAVRHLSSVDATLGRLIKRAGAFN
                     MQLRQLHDPFEALARNIIYQQLHGNAAAAIHGRVLSLFNKDRLRPQDILDAVEESLRG
                     AGLSGAKLAALKDLAAKTLDGTVPTLTRLRRMSDEEIIGHLTQVRGIGRWTVEMLLMF
                     RLGRPDVLPVGDFAVRKGFQLTYKLEEMPKPKALAEYGERWRPFRSVASWYMWQAIEL
                     DKQK"
     gene            4372..5553
                     /locus_tag="VGB76_15830"
     CDS             4372..5553
                     /locus_tag="VGB76_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100414.1"
                     /translation="MGLDIFKERGVPLEKQQFTWRDMVQSPISKLDDDAFTRVRIILM
                     NGLETEANRFSHACARMNRELQLPLARVRRVEQHQQTLVNWLLPADLSPIETTLAYEQ
                     VAIEVTASVALREPDEYMAQVYRFGLLEDFDHLYRFSALYDRVVGGDANNITQNYSDI
                     MPGRPTAVEHRDPSDDLREHYDKRMAAPLTKLHALTIMASENQTHDYYMNIGPLFADP
                     VARQLYAEIASIEEQHTTQYESIIDASETWMEKWLMHEAMEVYNYMGCSQYETNSRIK
                     AIWDRFLDYELGQLHFVMELFKQVENRDPAEVLPATLPEPIEYRSHREFVRETLAREV
                     DLRASGTQFVSKEDEPERSLAYRAQLNSEGSPTETVAAGYRWRPGTELNAGEAALGKG
                     A"
     gene            5557..6306
                     /locus_tag="VGB76_15835"
     CDS             5557..6306
                     /locus_tag="VGB76_15835"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0100415.1"
                     /translation="MDNRGTSSMEKTGWNRTGMVTAPVMGPEMVEGAAAAAPSSVGGT
                     EALLMRRGEYIREGFPLGSLPPPPVSLEGVVEGAAQVVGMTTGVATLMDKLSERLAFE
                     RTGTRLYDALIGKCAALGETSPGPTIAQLQEIREDELRHFMLVNRAITTLGGDPTVMS
                     PCADTVMVSSLGLIQVLTDPRTNVAQCLQAILTAELTDNDGWQLLINLAEELGHADLA
                     TEFRAALANEERHLLSVRGWLQEQVRAQAQA"
BASE COUNT         1132 a         1841 c         2113 g         1266 t
ORIGIN      
        1 tggcgcggca acgggatcga cgagcgacgg tttgatcgcc gcgttgcgct gcacggggct
       61 ggtctgaccc tgtggtcccg gcgcgccgag cacggagggg acacccgcga tcatttcgcc
      121 cgtcgtcgtg accagcacga agtcctgtgc gttgtcgccc gtgtcctgcg gcgtgccgct
      181 cgccgacttg cgcacgtagc tgtactgagg ccggagcgcg ggaaacgccg agatgaccgg
      241 cagccccgcg ccctcgcgga actccgccgg gtcgtccgcg aagccgacgg catcgacgtg
      301 cgcgttcact ccgttgcgcc gtagaccaat gccctgattg tcggagaaga agtccgcgcc
      361 cgtcgtgaag agattgccag cagcatacgc gctcaaacta tagcctgtgg agttcactat
      421 cagcctgtgg ctgcgcggcg tgagcaggaa cccgttctgc aatgtggcga acgagagggt
      481 cgtggggctt ccggcgctcg tagccaagaa gacgaagctg tagttgcgca ggtcaatgtt
      541 cgagttggtg ttgttgtaaa tctctatgta ctcgtcgttc gcgccgccgg gtgctgacaa
      601 tctgaattcg gtaataagca gttcgcccgt cgtgccggga tcaggatcgt tgtcgatgat
      661 cgtgccggag ccgaccggat tagagatggt cgcgttgacg gcgttcgtga ggatgacgac
      721 gaatctttcg ttctgttcgt caatcgtgtc ggcgatgatc ggcacggcga tcgctttggt
      781 ggtctggccg ggcgtgaacg ttatctgcaa gttgagcggc gtgtaatcgg cgttggcctg
      841 cgccgtatcg tcgtcggtaa agtaccggac ggtcacagtg ttgctgctcg gctcggagag
      901 acggatggtg aagatggcgt ttgccccgct gttggcagtc tcttcgttga tggtgacgtt
      961 gttgatggag acttcgggga gcggatcgtc gtccgtgatg ttgagcacgg cgtagatcgg
     1021 gttgccgagc gtgctgcccg tggcattgct caggaacagg ttgacggttt ccgtagtctc
     1081 gccgagcgaa tcgtcgagaa tctggatggt gaaactcttc atggattcgc cgggggcgaa
     1141 aaagagcgtt ccggaggcgg gcgtgtagtc agcgttggcg gtcgccgagc cgttgctcgt
     1201 cacgtaattg acgctcgccg tctctgccgc gccgaatgtg cgcacgacgg taatgacgac
     1261 gcttccgctc gtctccgcag tcgtgtaact gctcgcagag aactgcacgt gtccgggctg
     1321 atctacttcg tggcagacgg agaattcgga cgtgttgccg ctcgcatcgg tcgccgtcgc
     1381 cgtgacgctc tgccccggca ggagttggac ggggaaggtc gcattgaaag cagcgttacc
     1441 gctcgcgtcg gtgttcaccg tcgtcaggcc gagaaggaac tgcccctcgc cctgtcccga
     1501 agcatcacaa ctgttgttgg cgaacacctc gatgatgtag ctcgcgttcg gcgcgctgtt
     1561 gagcgtgccc tgcgcgtttg tgctgccgcc gcccgtcgag acggaacgca cgaggggaaa
     1621 gttctgtaga ttgttggagc cggtgtcggg gtcgcccgcg tcgttgggtg tgacgcccgc
     1681 cgcgccgagg tcgatgccga gttggccgtt ggagaagatc gagttggcgc ggacggtgtg
     1741 gcctgtcccc gtagtgttga ctaagacgcc ggtgcccgcg ttgaacgcga tgacgttggc
     1801 tgcgcccggc tcgctgccgc cgacgatgct gttaacgctg gtgctgtctc cgagaatcga
     1861 cacgccgtgc atgctgttgc cgagtccggc tccggcggcg ttcgtgccga tgtagttgcc
     1921 ctgcaccttc gtattggtgg agctgaggat ttggatggcg ttgccggtgt tggcggcgcc
     1981 gcccgtcgta ctcgcgacga cgttgcccgc acctgcggtt gtgcccccga tgagcgcgtt
     2041 cacgttgccg gagatgctca aaccgaagac ggcattgccc agcggcgcgc tgcccgcagc
     2101 ggtggtgccg atgtagttgc cctgtaccgc actgtcgctt gtgccctgca tctggatgcc
     2161 gaaattgttc cccgagacga cgttgcgcgc cccggccaca gtgccgccga tgacggtgcc
     2221 ggacgcgttg aaaacggcga tgccggtgag gttgcccaga tcgcgcgagc cggcggcgtc
     2281 cgtgccgatg tagttgcctt gaatcgcgtt gccgttcgag cttgcgtcct gaatggtgat
     2341 gccgacgttg ttgcccgaga tgacgttgcg cgccccggcc acagtgccgc cgatggtgtt
     2401 gttgggcgcg gagatgaaga cgccgctgtg ggtgttgctg agttcggcgg tgccggcggc
     2461 gttggtgccg atgtagttgc cctgcaccgt gttgttcgtt gtagcgttga agatcgagat
     2521 gccgtgcgag tcagtggcag agatgacgtt gcgtgcagcg ggcgtcgtcc cgccgatgag
     2581 gttgtctgag gaactgacgc tgatgccgtt gccgcgactg ttggtgctgc tcgtaccggc
     2641 agcgttcgtg ccgatgtagt taccctcgac caggttgccg ccagcggtct gcaagttgat
     2701 gccgccatca gagaagccat tgatgacgag gccgcgcacg acgctattgc cagccgtgat
     2761 ctggagcgcc gcgctgttcg ggttggcgga gaagccgcta cagttcaatt cgataaccgg
     2821 ctgccctgca aagcccggct gcgtcgtggc gtcaataacc agcgggtcgg tgatcacagg
     2881 cagttgcgcc gtcggcatga tggtctgtag ccctgagctg atggcgaagt tgatcgtatc
     2941 cctgcccgtc gtggcattgg cctgcatgat cgccgcgcgc agcgtgcagt tgcccgcgcc
     3001 gtcgttgcag acgccgtcgg tcgtgtcgct gtcggggcta tcgcctgtgg agttgacgtt
     3061 gaaagtcgtc gccgcttgaa ccgcctgaac ctccggctca aacgctttca gaaagcgatc
     3121 cggcaacatg aacagagcga gcagtccgaa ggtgaaggct ataaaagagg tgcgccgggc
     3181 acgggtatag agatacttac gcgagggggc gggttgcatg ggctttctcc tgagtaatac
     3241 cttgcggtaa atctgtcggt gcgttggaat taatgcggcg ttacgaactt atgcggccat
     3301 aagtttttgg tggccgtgat ggctgcgcag gagactgtac attatcgtca gcgcaggatc
     3361 aattcgagcc gcacgccttc cgggcgagcg caaaagggtt tcggagtctg aaagtttgga
     3421 cttcgacgcc gtgttgataa gctcattttt gcttatccag ttcgatggct tgccacatgt
     3481 accaactcgc cacggagcga aagggtcgcc agcgttcgcc gtactctgcg agagccttcg
     3541 gcttcggcat ctcctcaagt ttgtaagtta gctggaagcc ttttcgcacg gcgaagtcgc
     3601 cgacgggcag aacgtccggt cgtcctaagc gaaacatcag gagcatctcc accgtccagc
     3661 gtccgatgcc gcgtacctgt gtgaggtgcc ctatgatctc ttcgtcgctc atccgccgca
     3721 gacgcgttaa ggtgggcacg gtgccgtcca gtgttttagc ggcgaggtct ttcagcgcag
     3781 cgagctttgc cccggataga cctgccccgc gtaagctctc ctccacagca tcgagaatgt
     3841 cctgcgggcg cagtctatct ttattgaaga gcgacaggac gcgcccgtgg atcgccgccg
     3901 cggcattacc gtgaagctgc tggtagatga tgtttcgcgc cagcgcttcg aaaggatcgt
     3961 gcaactggcg gagctgcata ttgaacgccc ccgcacgctt gatcagcctg ccgagagtgg
     4021 catcaaccga cgagagatgt cgcacggctt catctgcgtc gtagagaaag ccgtccttct
     4081 ccctttcaat agttttactc tttcgtttca tgtcgaaacc cgttaagagc agttgacaga
     4141 gcgtgagggt gagataatac ccgaatagct atttttgcaa catgccgcca caggcgcact
     4201 gtgcataaac gtacagacgt tcgcgcgtgc gggacatata gtttggcctt tccacttcgc
     4261 gttatttaat aaatgaaggt gcaagttagc cgccggactg cgtcgggctg cgcccgcatt
     4321 cgtgcgccgc gatgaccagc tttgcggaga gcttgacgga aggagcgagc gatgggtttg
     4381 gacatcttta aagagcgggg cgtcccgctc gaaaaacaac agttcacgtg gcgcgacatg
     4441 gtgcagtcgc ccatcagcaa actcgacgac gacgcgttca cgcgcgtgcg tatcatcctc
     4501 atgaacgggc tggagacgga ggccaaccgc ttcagccacg cctgcgcgcg catgaaccgc
     4561 gaactgcaac tgccgctcgc gcgcgtgcgc cgcgtggaac agcaccagca gacgctggtt
     4621 aactggctgc tgcccgccga cctctcgccc atcgagacga cgctcgccta cgagcaggtc
     4681 gccatcgagg tcacggcgag cgtcgccctg cgcgagccgg acgagtacat ggcgcaggtc
     4741 taccgcttcg gcctgctcga agacttcgac cacctctatc gcttctccgc cctctacgac
     4801 cgcgtcgtgg gcggcgatgc caacaacatc acgcagaact actcggacat catgccgggc
     4861 agacccaccg cggtcgagca ccgcgaccct tcggacgacc tgcgagagca ctacgacaag
     4921 cggatggccg cgccgctcac caagctccac gccctgacca tcatggcgag cgagaaccag
     4981 acgcacgact actacatgaa catcggccca ctcttcgccg accctgtcgc gcgccagctc
     5041 tacgccgaga tcgcctccat cgaggagcag cacacgacac agtacgagtc catcatagat
     5101 gcctcggaga cgtggatgga gaagtggctg atgcacgagg ccatggaggt ctacaactac
     5161 atgggctgct cgcagtacga gactaactcg cgcatcaagg cgatctggga tcgcttcctc
     5221 gactacgagt tggggcaact ccacttcgtc atggaactct tcaagcaggt ggagaaccgc
     5281 gacccggcgg aggtgctgcc cgccacgctg ccagagccga tagagtatcg cagccaccgc
     5341 gaattcgtcc gcgagacgct cgcacgcgag gttgacctgc gcgcttccgg cacgcagttc
     5401 gtcagcaagg aagatgagcc ggagcgttcg ctcgcgtatc gcgcgcagtt gaactccgag
     5461 gggtcgccga ccgagacggt ggccgcgggc tacaggtgga ggccggggac agagttgaac
     5521 gcgggcgagg ccgcgttggg gaaaggagcc tgagcaatgg ataacagggg tacgagttcg
     5581 atggagaaga cgggttggaa ccggacgggg atggtgaccg cgcccgtgat gggacccgag
     5641 atggtcgagg gcgcagcggc ggctgctccg agttccgtgg gcgggacgga ggcgctcttg
     5701 atgcggcgcg gcgagtacat acgcgagggc ttccccctcg gctccttgcc gccgccacca
     5761 gtctcgctcg aaggcgtggt cgagggtgcg gcgcaagtcg tcgggatgac gaccggcgtg
     5821 gcgacgctga tggacaagct cagtgaacgg ctggctttcg agcgcacggg cacgcgtctc
     5881 tacgacgcgc tcatcgggaa gtgtgctgcg ctcggcgaga cttcgccggg gccgaccatc
     5941 gcgcagctac aggagatacg cgaagacgag ttgcgccact tcatgctggt caatcgcgcc
     6001 atcacgacgc tcgggggcga cccgacggtg atgagtccgt gcgcggacac ggtgatggtt
     6061 tcgtcgctgg gactcataca agtcttaacc gacccgcgca ccaacgtagc gcagtgcctt
     6121 caggccatcc tgacggcgga gttgaccgac aacgacggct ggcaattgct catcaacctc
     6181 gcggaggagt tggggcacgc cgacttggcg acggagttcc gcgcggcact cgcaaacgag
     6241 gagaggcacc tcttgagcgt gcgcggctgg ttgcaggagc aggtgcgggc gcaggcgcag
     6301 gcttgagccg ggtaagagtt aatcacggca gacacggagg cgcggcttgt ag
//
LOCUS       DASWNS010000462        11723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_485943, whole genome shotgun sequence.
ACCESSION   DASWNS010000462 DASWNS010000000
VERSION     DASWNS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11723
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485943"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1396
                     /locus_tag="VGB76_15840"
     CDS             149..1396
                     /locus_tag="VGB76_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100416.1"
                     /translation="MRRPLLLSAILLLSACGACREVAHAKQKPPPVPPATPVTMAELN
                     TRRTSGVSVVEVSDYVYGGYSGALQSPPHADLNPRKAFVIFWKDFNYRFVFSHEASYC
                     PWFEMPEGAAVSYQMFEGNEGWAELFNEQGRRERNSFVDIIERGHRRVWVRWTYFGVN
                     MTTGEAAFRAVEDFWAYPNGLILRRQRYHTLMPGDPRGYAREPIELIVLSPVGKLWHD
                     VLAAEPSTGESRAFVGVDAFSHARVDIFWKRRDDPKKIFAGTSRRTGSPWKEIDDARG
                     FAGIIPLGVGSPFFVIGDASGFPREGTRLKEHSDRKGTGGWGWGTLTWDHWPIGWLNS
                     QAHDLDAETFKRYPSHFAPFGLDLWSMPNEATEGRDFHSLLGVGGRDVEAIRALAKSW
                     LSGNASDPERAATHRPLPDAPRR"
     gene            complement(1546..2535)
                     /locus_tag="VGB76_15845"
     CDS             complement(1546..2535)
                     /locus_tag="VGB76_15845"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY0100417.1"
                     /translation="MSRRRATFATGALVVQLLLTLAGCGGSGGRPPAQLSAGSTEAAP
                     LAGSHGATRGDAGAHVPAPAGMVLIEGGTFRMGSDAEMPDESPAHQVSVKSFWIDRDE
                     VTVGEFARFVDATGYTTEAERFGWSGVFDIASGEWQRRDGADWRHPEGLEAAPAAMDE
                     PVTQVSFADASAYARWAGKRLPTEAEWEYAARGGRENSRYAWGDELRPGGKPAANWWQ
                     GKFPERNTVEDGFAARAPVGSFAPNGYGLRDVGGNVWEWCADWYEAGYYAKGDATENP
                     QGATTGTERVIRGGSWMCSENYCTNYRVAARGHTAPDSGLNNLGFRCARDLAE"
     gene            2724..3713
                     /locus_tag="VGB76_15850"
     CDS             2724..3713
                     /locus_tag="VGB76_15850"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0100418.1"
                     /translation="MTSDAIAAIDIGGTKIALGLAPLDGQPLPFRRFPTRIKNAPEQI
                     LEEACSELERMAGEAGVHLVAVGIGCGGPLDRRRGLILSPPNLPRWDEFPIVRQVEER
                     FGIPVLLDNDANAAALGEHEYGAGRGLRNLVYITISTGIGGGLIIDNRLVHGIGDGAG
                     EVGHMVVAPGGRPCGCGARGCLEAICSGTSIARRALERLALDEQPSTLRAGGEQITAH
                     DVAEAARDGDELARAVWDETLDYLALGLSNIIATLAPEAIILGGGVSTSGDQLLVPLR
                     ERVCASVKIMPVEQVRILQASLGGDSGVYGALIIGRRALAARRANDEAMTVHP"
     gene            3796..5613
                     /locus_tag="VGB76_15855"
     CDS             3796..5613
                     /locus_tag="VGB76_15855"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0100419.1"
                     /translation="MKQNLRTFNCSFNLISLLLLLTAFVLCSALAAPAATPSQQSQRA
                     GRSANARRTVRGAQPEWWKRAVFYEIYPRSFKDSDGDGIGDLNGITSKLDHLAALGVD
                     AIWITPFYPSPQVDFGYDVSDYENVDPQFGTLRDFDRLVAAAHKRKIKVVIDFVLNHT
                     SDQHPFFIESRSSRTSAKRDWYIWHDPKPGGGRPNNWSSSFGPVAWTLDERTGQYYYH
                     YFYTQQPELNWRNPEVERRMLETVRFWLRRGADGFRLDAVNYLYEDPQLRDNPVLKEL
                     RFGSTTEYEQEKKYNRDLPEVQDAMVRLRAFNDSVNPESVLIGEAYVPKWEELMRYYG
                     PSNNGVHLPFNFFLVMEPARTQLKASVFRDVIAASERALAGRWTTYVLSNHDIPRAYD
                     RLGDGQHNDQIAKLLATMLLTLRGSPFLYYGEEIGMLTTEPKTVEEVRDPVGRRYWPL
                     RKGRDGERTPMQWDATAHAGFTTGVPWLPVPPSARTRNVVAQTADGSSLLNFYKQLIR
                     LRRRSPALLDGDYRSIGADPHVYAYLRRAPGQTVLVALNMSADNRTFQLGDAAPAKAS
                     ALRVRLSNAARTGERISGRELRLAPFEAVVLELGADEKF"
     gene            5683..8400
                     /locus_tag="VGB76_15860"
     CDS             5683..8400
                     /locus_tag="VGB76_15860"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY0100420.1"
                     /translation="MTFSRTKCFATVNRQISRAWTALLQFALILSLCLPATAQAQANA
                     AAGGAPPDYLNPQLPIERRVADLVGRMTLEEKVAQMMNKSPAIARLGVPDYDWWNEAL
                     HGVAYAGNATVFPQSIGLGATWNPALMQRVATAISDEARAKYHEAVRRDFRKRFYGLT
                     FWSPNINIFRDPRWGRGQETYGEDPFLTSRLGVAFVRGMQGDDPRYLKVIATPKHYAV
                     HSGPEPERHVFDAAVNERDFRETYMPAFQAAIVEGKAGSVMCAYNRVNGEPACASKRL
                     MTDILRTEWGFDGYVVSDCDAVLDIYKNHKFAKTEEEGVALAVKAGTELTCGYEYRAL
                     IPAIKAGHISEAEIDTAVRRLFTARFRLGLFDPPEMVAYSRIPFEVNDSAAHRELARD
                     AARESLVLLKNENRQLPLRRDLKTIAVIGPNADSLEVLLGNYHGIPSKWTTPLEGIRR
                     KVSPATRVLYALGTTLTGEALLPVPADALSQPDGKPGLRAEYFANKDLQGAPVATRVD
                     SQINFDWFTNAPVPELPLDNFSARWTGQLTAPTSGTYELGVRADDAARLYLDDQLFLD
                     GWRDGSAKTVRKPLAMEAGRAYKIRVEFYDRYAAATAKLLWGPPKLAESLYEDAIRKA
                     KEADVVVMTLGLAPSLEGEEMDVKLEGFRGGDRTELNLPKVQEELLKAVHATGKPVVL
                     LLFSGGGISVNWADANVPAIVQAWYPGEEGGAAIADMLFGDYNPSGRLPVTFYQSASQ
                     LPPFADYSMQGKTYRYFKGEPLYPFGHGLSYTKFEYDKLRVKRSVRAGEPVEVSAEVR
                     NSGALAGDEVVQLYVTDVAASAPVPLRSLRGVQRINLRPGERRRVSFTLTPRDLTLID
                     DGGKRILEPGEFTVTLGGKQPGFKGRADTATTGVVSGSFVVKGKKLELP"
     gene            8448..10841
                     /locus_tag="VGB76_15865"
     CDS             8448..10841
                     /locus_tag="VGB76_15865"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEY0100421.1"
                     /translation="MNRKHQTTFASLVISVLLSLNASVIPVRASAFAAASTADPAQYV
                     NPFVGTGNSPLPDYLGGNASGNTFPGATLPFGMVQWSPDTERGFGKDERGSYLYHDPT
                     IRGFSLTHLSGPGCPVFGDVPIMPVSGGLTVSPAIEPNAYHAKFSHERESASPGFYEV
                     ALDTGVRVSLSATERTGFGVFTYPDSQAATLLFDVGRNASGVTEGTWQRDGDRRVSGS
                     VTSGGFCGTRNKYKLYFAAEFDRPFVTTGTWEGERISHGGRSVKGAKVGGFVMFDPAS
                     ERAVRVKVGVSYVSAENARLNLRAENAGWNINAVRARARARWNDALSRIEIGGGTLPQ
                     RQVFYTALYHTLLHPTIFSDANGEYLGFDDKVHASRTHTQYTNFSGWDIYRSQIPLVA
                     FLFPEEASDMMQSLVNAAQEGGGLPIWPVANDESGAMYGDPATASIASAYAFGARNFD
                     TRAALAAMIKNATDPETKSRTYPARPALADYLKYGYIPMEQKGLRGSPSVALEYQTAD
                     FALAQFARAHGDTATHREMMRRAQLWTRLFDPVNKYIRGRWRDGSWLPGFDFKEMLYK
                     PELPWRPESHKSYVEGNAVQYTWMIPHNMRTLFDRIGGNDVVVRKLDDFFKEFNAGPD
                     RPYFFIGNEPVFPVPWAYNYAGQPWKTQQITRRVLTELFTNTPGGLPGNDDLGATSSW
                     IVFAAIGMYPVTPGVGGFSLNSPLFPEIKIRMGGRGGKSLRIVGDGASTGTPYVQELR
                     LDGKPHESTWLPFERISRGATLRFKLGATPNTQWATAPAAAPPSFPEGMESASAPGQ"
     gene            complement(11023..>11723)
                     /locus_tag="VGB76_15870"
     CDS             complement(11023..>11723)
                     /locus_tag="VGB76_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100422.1"
                     /translation="LWSPRETRDVEDMYRRLEAVAVQLEEFGLTHRTYAGKMLRRLAG
                     SERVEPLETLTGTLAPKFFAREHVRPITQMTPLTRLVDAVAPDSRAAREFASAVGALL
                     EDAPRFESRRERVRGFLDGWRGVYAPVRVMADRSPLMRDAEPLAGNLSEMSEAGLEAV
                     QYLSAGIAPPAGWREEKLVLLERIAKMPREVEFTIIPSLRRLVFAAAELPQLRTLTAA
                     DWKASVLKLADEKR"
BASE COUNT         2151 a         4025 c         3573 g         1974 t
ORIGIN      
        1 tttacgatta aggaggtcaa tttatttttt gcgcgccgcg aaattcagcc gccccgtcgc
       61 ggcttccgcg cgatgtggta tgtttgggcg gaaggctttt tcccgcttcg cccgtcctga
      121 tcccaacgct taaggagaac ctcttcggat gcgtcgcccg ctactgctca gcgcaatact
      181 tctgctctcc gcctgcggcg cgtgccgcga ggttgcacac gctaaacaaa agccgccgcc
      241 cgtcccgccc gccacgcccg tcacgatggc ggagttgaac acacgccgga cgagcggggt
      301 ttccgtcgtt gaagtgtcgg actatgtcta cggcggctac agcggcgcac tccaatcgcc
      361 gccgcacgcc gacctgaatc cgcgcaaggc tttcgtcatc ttctggaaag atttcaatta
      421 ccgcttcgtc ttttcgcacg aggccagcta ttgcccgtgg ttcgagatgc cggagggcgc
      481 agccgtctcc taccagatgt ttgaaggcaa cgagggctgg gctgaactct tcaacgagca
      541 ggggcgacgc gagcgtaaca gtttcgtgga catcatcgag cgcggacatc gccgtgtgtg
      601 ggtgcgctgg acctacttcg gcgtcaacat gacgacgggc gaggcggcct ttcgcgccgt
      661 cgaagatttc tgggcttacc cgaacggcct catcctgcgt cgccagcgtt accacacgct
      721 catgcccggc gacccgcgcg gctacgcgcg cgaacccatc gaattgatcg tgttgagtcc
      781 ggtcggcaaa ctctggcacg acgtgctggc cgccgaaccc tcgaccggcg agagccgcgc
      841 cttcgtcggc gtggacgcct tctcgcacgc gcgcgtggac atcttctgga agcgcaggga
      901 cgatccaaaa aagatcttcg caggaacttc gcgccgcacg ggttcgccct ggaaagagat
      961 cgacgacgcg cgcgggttcg caggcatcat tccgctcggc gtcggctcgc cgttcttcgt
     1021 catcggcgac gcttccggct tcccgcgcga aggcacgcgg ctcaaagaac actccgaccg
     1081 caaaggcacc ggcggatggg gctggggcac gctcacgtgg gaccactggc ctatcggctg
     1141 gcttaacagt caggcgcacg atctcgacgc cgaaaccttc aaacgctacc ccagccactt
     1201 cgcgcccttc ggcctcgacc tgtggagcat gccgaacgaa gccaccgagg ggagagactt
     1261 ccattcgctg ctcggcgtcg gcgggcggga tgtcgaagcg atccgcgcgc tggcgaaaag
     1321 ctggctcagt ggaaacgcga gcgatcccga acgcgccgcg acgcaccgcc cgctccccga
     1381 tgccccgcgt cgttagagat gattactgac aaagctctga gccgccgtcg ttcaacgaac
     1441 tgaaagcagc gcggccaccg gcctgaaacg atgtgtcgga agaacgtctg gaaagatgta
     1501 tgggaagaac ggctggcgaa tcgtcaacga atcgtcaagg catctctact ctgcgaggtc
     1561 gcgggcgcag cggaagccca gattgttcaa gccgctgtcg ggcgcggtgt gcccgcgcgc
     1621 ggcgacgcgg tagttcgtgc agtagttttc gctgcacatc cacgaaccgc ccctgatcac
     1681 gcgctccgtg cctgtggtcg ccccctgcgg gttctctgtg gcgtcgccct tcgcgtagta
     1741 gcccgcctcg taccaatcgg cgcaccactc ccagacattc cctcccacat cgcgcaggcc
     1801 gtaaccgttc ggcgcgaaac tgccgacggg cgcgcgtgcc gcgaagccgt cctcgactgt
     1861 gttgcgctcc ggaaacttcc cctgccacca gttcgcggcg ggcttaccac cgggacgcaa
     1921 ctcgtcgccc catgcgtacc tcgaattctc ccgcccgccg cgcgccgcgt actcccattc
     1981 cgcttccgtg ggcagacgct tgcccgccca gcgcgcgtag gcggaagcgt ctgcaaagga
     2041 gacttgcgtg acgggttcat ccattgctgc gggcgcggcc tcaagccctt ccgggtgtcg
     2101 ccagtcagcc ccgtcgcgcc gctgccactc acccgaagca atgtcgaaga cgccagacca
     2161 gccgaagcgt tcggcctctg tcgtgtagcc ggtcgcgtct acgaaacgcg cgaactcgcc
     2221 gacggtgact tcgtcgcggt cgatccagaa ggacttgacc gacacctgat gtgcgggcga
     2281 ctcgtccggc atctccgcgt cagaacccat gcggaacgtc ccgccctcga tcaagaccat
     2341 tcctgccgga gcaggcacgt gcgcgcccgc gtcgcctctc gtcgcgccat gacttccggc
     2401 taacggcgcg gcctctgtgc tgcctgccga aagttgcgcg ggcggccttc cgccactgcc
     2461 gccgcagccc gcgagcgtca ataaaagctg caccaccagc gcgcccgtcg caaatgtcgc
     2521 acgtctacga ctcatacgga cgggagatta tgttccgccc acgcatcagt caagaaggaa
     2581 atcgaggcgg atgcggaacg cgcgcccgga tcaattttcc tctcgaccgg cgggacgatt
     2641 ccgccgccct aatttgacaa aggcgaggcg aatcgcatac aagcgaatcg ctaccccacg
     2701 ttgattctac cgactaatcc gaaatgacca gtgacgctat cgcagccatc gacatcggcg
     2761 ggacgaaaat tgcgctcgga ctcgccccac tcgacggcca acctttgccc ttccgccgct
     2821 ttcccacgcg gatcaaaaac gcccccgaac agattttgga agaggcttgc agcgaactcg
     2881 aacgaatggc cggagaggcg ggcgtgcatc tcgtcgccgt cggcatcggc tgcggggggc
     2941 ctctggaccg gcgacgcggg ctgatccttt cgccgccgaa ccttccccgc tgggacgagt
     3001 ttcccatcgt ccgtcaggtc gaagagaggt tcggcattcc agtccttctg gacaacgacg
     3061 ccaacgccgc cgcgctcggc gagcacgagt atggggcggg acgcggcctg cgcaatctgg
     3121 tctacatcac gatctcgacc ggcatcggcg gcggcctcat catcgacaac cgactcgtgc
     3181 atggcatagg cgacggcgcg ggcgaggtcg ggcacatggt ggttgccccc ggaggccgtc
     3241 cgtgcggctg cggtgcgcgc ggctgcctcg aagcgatctg ctccggaacc agcatcgccc
     3301 gccgcgctct ggaacgtctc gcactggacg agcagccttc gacgctgcgc gcgggcggcg
     3361 agcagatcac cgcgcacgat gtcgccgagg cggcgcgcga cggcgacgaa ctcgcacgcg
     3421 cggtgtggga cgaaacgctc gactatctcg cgctcggtct gagcaacatc atcgcgacgc
     3481 tcgcgcccga agccattatc ttaggcgggg gcgtctccac ctccggcgat caactgctgg
     3541 ttcctttgcg cgagcgcgtg tgcgcgtcgg tcaagatcat gcccgtcgaa caggtgcgga
     3601 tattgcaagc gtcgctcggc ggagacagcg gcgtctacgg cgcgctgatc atcgggcggc
     3661 gcgcgctcgc cgcacggcgc gcaaacgacg aggccatgac cgttcatccc tgaacagatc
     3721 gcgcgcgtgg cgttcgcctg cccggcgcat tcgtctccgg ctccaaacca actcgatgtc
     3781 gaggttcatt cgccaatgaa gcagaacttg cggacgttca actgttcgtt caatctcatc
     3841 tctctcttac tgctcctgac ggccttcgtc ctctgctcgg cgctcgcggc tccggcggcc
     3901 acgccgtcgc agcagtcgca gcgggcaggg cgcagcgcaa acgcgcgccg gacggtgcgc
     3961 ggcgcgcagc cggagtggtg gaagcgcgcg gtcttttacg aaatctaccc gcgcagtttc
     4021 aaagactccg acggcgacgg catcggcgat ttaaacggca tcacctcgaa gctcgatcat
     4081 ctggccgcgc tcggcgtaga cgccatctgg atcacgccct tctacccttc gccgcaggtg
     4141 gacttcggct acgacgtgag cgattacgag aacgtcgatc cgcagtttgg cacgcttcga
     4201 gacttcgacc gcctcgtcgc cgccgcacat aaacgtaaga tcaaagtcgt catcgacttc
     4261 gttctcaacc acacgtccga ccagcacccc ttcttcatcg agtcgcgctc gtcgcgcacg
     4321 agtgcgaagc gcgactggta catctggcat gacccgaagc ccggcggcgg gcgaccgaac
     4381 aactggtctt cgtccttcgg acccgtcgcg tggacgctcg acgagcgcac agggcagtac
     4441 tactaccact acttttacac gcagcagccc gaattgaact ggcgcaaccc ggaggtcgaa
     4501 cggcggatgc tggagacggt gcgcttctgg ctgcggcgcg gcgcggacgg cttccggctc
     4561 gatgccgtca actacctcta cgaagacccg caactgcgcg acaaccctgt gctcaaggag
     4621 ttgcgcttcg gcagcacgac ggaatacgag caggagaaga agtacaaccg cgacctcccc
     4681 gaagtgcagg acgcgatggt gcgcctgcgc gccttcaacg acagcgtcaa ccccgagagc
     4741 gtgctcatcg gcgaagccta cgtgccgaag tgggaagagt tgatgcgcta ctatggtcct
     4801 tcgaacaacg gcgtccacct gcccttcaac ttctttctcg tgatggaacc ggcgcgcacc
     4861 caactcaagg cgtccgtctt ccgcgatgtg atcgccgcga gcgagcgcgc actggccgga
     4921 cgctggacta cttacgtgct ctccaaccac gacatcccgc gcgcctacga ccgcctcggc
     4981 gacgggcaac acaacgacca gatcgccaaa ctgctcgcca ccatgctgct caccctgcgc
     5041 ggctcgccct tcctctatta cggcgaagag atcggcatgt tgacgaccga accgaagacc
     5101 gtcgaagagg tgcgcgaccc cgtcggtcgc cgctactggc cgttgcgcaa ggggcgcgac
     5161 ggcgagcgca cgccgatgca gtgggacgcg acggcgcacg cgggctttac gaccggggtg
     5221 ccgtggctgc ccgtcccgcc ctcggcgcgc acgcgcaacg tggtcgcgca gacggcggac
     5281 ggttcatctc tactcaactt ctacaaacag ttgatccgcc tgcgccgccg cagccccgcg
     5341 ctgctcgacg gcgactaccg gagcatcggc gcagacccgc acgtctacgc ctacctgcgc
     5401 cgcgcccccg gccagaccgt gctcgtcgcg ctcaacatgt ccgccgataa ccgcacgttt
     5461 caactcggcg atgcggctcc cgccaaagct tccgcgctgc gtgtgcgcct gagcaatgcg
     5521 gcacgcaccg gcgagaggat cagcgggcgc gagttgcggc tcgcgccatt tgaagcggtc
     5581 gtgttggagt tgggagcgga tgagaagttc tgaacgcgta tagccttccg ggcttcgggt
     5641 aaagagtcgg gtaaagagat caagtttagc aggagagaac ggatgacttt ttcgcgcact
     5701 aaatgtttcg ccacggtcaa cagacagatt agccgcgcgt ggactgcgct gcttcagttc
     5761 gcacttatct tatctttgtg tctcccggcg acggcgcagg cgcaggccaa cgcagcggcg
     5821 ggcggcgcgc cccccgatta tctcaacccg caacttccga ttgagcgcag ggtggccgat
     5881 ctcgtcgggc gcatgacgct cgaagagaag gtggcgcaga tgatgaacaa gtctccggcc
     5941 atcgcgcgcc tcggcgtccc cgattacgac tggtggaacg aggcgttgca cggcgtggcc
     6001 tacgccggga acgccacggt ctttccgcag tccatcggcc tcggcgcgac gtggaacccc
     6061 gcgctcatgc agcgcgtcgc cacggccatc tcggacgagg cgcgcgccaa gtatcacgaa
     6121 gccgtcaggc gcgacttcag gaaacgcttt tacggtctga ccttctggtc gccgaacatc
     6181 aacatcttcc gcgacccgcg ctgggggcgc gggcaggaga cttacggcga agaccctttt
     6241 ctcacctcgc gtctcggcgt cgccttcgtg cgcgggatgc agggcgacga cccgcgctac
     6301 ctgaaagtga tcgccacgcc caagcactac gcagtgcata gtggacccga acccgaacgc
     6361 cacgtcttcg acgccgccgt taacgaaaga gactttcgcg agacgtacat gcctgcgttt
     6421 caggcggcca tcgtcgaggg caaggccggg tctgtgatgt gcgcttacaa ccgcgtgaac
     6481 ggcgaaccgg cctgcgcgag caagcgtctg atgaccgaca tcctgcgcac cgaatggggc
     6541 ttcgacggct acgtcgtctc cgactgcgac gccgttctcg acatctacaa gaatcacaag
     6601 ttcgcaaaga ccgaggagga gggcgtcgcg ctcgccgtca aagcggggac ggagttgacc
     6661 tgcggctacg agtaccgcgc gctcattccg gccatcaagg cggggcatat cagcgaagcg
     6721 gagattgata cggcggtcag acgcctcttc accgcgcgct tccgcctcgg cctgttcgac
     6781 ccgcccgaga tggtcgccta ctcgcgcatc cctttcgaag tgaacgactc cgccgcgcac
     6841 cgcgaactgg cgcgcgacgc ggcgcgcgag tcgctcgtcc tcttgaagaa cgagaaccgc
     6901 cagcttccgc tccggcgcga cctcaagacc atcgccgtca tcggaccaaa cgccgactcg
     6961 ctcgaagtgc tgctggggaa ctatcacggc atcccttcaa aatggactac gccgctcgaa
     7021 ggcatccggc gcaaagtgtc gcccgcgacg cgtgtgctct acgcgctcgg cacgacgctc
     7081 actggcgaag cgctccttcc cgtccccgcc gacgcgctct cgcagccgga cggcaagccc
     7141 ggcctccgcg ccgaatactt cgccaacaaa gatttgcagg gcgcgcccgt cgcaacccgc
     7201 gtagactctc agatcaactt cgattggttc accaacgcgc ccgtgcccga actgccgctc
     7261 gataatttca gcgcgcgctg gacgggacaa ctcaccgcgc ccacttccgg cacgtacgaa
     7321 ctcggcgtgc gcgccgacga tgccgcgcgc ctctacctcg acgatcaact cttcctcgac
     7381 ggctggcgcg acggcagcgc gaagaccgtc aggaagcctc tggcgatgga agcgggacgc
     7441 gcttataaaa ttcgcgtcga gttctacgac cgctacgccg ccgcgaccgc caagctcctc
     7501 tgggggccgc ccaaacttgc cgagtcgctc tacgaagacg ccatccggaa ggctaaggaa
     7561 gcggacgtgg tggtgatgac gctcggcctc gcgccgtcgc tcgaaggcga agagatggac
     7621 gtgaagctgg aaggattcag aggcggcgac cgcaccgagt tgaatttgcc gaaggtgcag
     7681 gaagagttgc tcaaagccgt ccacgccacg ggcaagccgg tcgtgctcct gctcttcagc
     7741 ggcggcggca tcagcgtcaa ctgggctgac gcaaacgtgc ccgccatcgt gcaggcgtgg
     7801 tatccgggcg aagagggcgg cgcggccatc gccgacatgc tcttcggtga ctacaaccct
     7861 tcgggcaggc tgcccgtcac gttctaccag tccgcgtcgc agttgccccc gttcgcagac
     7921 tacagcatgc agggcaagac ctatcgctac ttcaaaggcg agccgctcta tcccttcggc
     7981 cacggcctga gctacacgaa attcgagtac gacaagttgc gtgtgaagcg gagcgtgcgc
     8041 gcgggcgagc cggtcgaggt gtcggcggag gtacgaaaca gcggcgcgct cgcgggcgac
     8101 gaggtggtgc agttatacgt aacggacgtg gccgccagcg cgcccgtgcc cttgaggtcg
     8161 ctgcgcggcg tccagaggat caacctgcga ccgggcgagc ggcggcgcgt cagcttcact
     8221 cttacgccgc gcgacctgac gctcatagac gacggcggca agcgcatcct cgaacccggc
     8281 gaattcaccg tcacgttagg cggcaagcag ccgggcttca agggtcgcgc cgacacggcg
     8341 accacgggcg tcgtctccgg ctctttcgtc gtgaagggga agaagctcga acttccctag
     8401 cgcgcttttg agaactgtca ccaacttata cttttccacg aagcaagatg aacagaaaac
     8461 atcaaaccac gttcgcatcg ctcgtcatct cggttctcct gtcgctcaat gcgtcagtca
     8521 ttccggttcg cgccagcgcg ttcgccgccg cgtctacggc tgaccccgcg cagtacgtca
     8581 accccttcgt cgggacgggc aactcgccgc tgccggacta tctcggcggg aacgcgagcg
     8641 gcaacacctt ccccggcgcg acgctgcctt tcggcatggt gcaatggagt ccggacacgg
     8701 agcggggctt cggcaaggac gagcgcggca gctacctcta tcacgacccg actatccgtg
     8761 gcttcagcct cacgcacctg agcggccccg gctgccccgt cttcggcgac gtgccgatca
     8821 tgcccgtctc cggcgggctg acggtttcgc ccgccatcga accgaatgct tatcacgcga
     8881 agttctcgca cgaacgcgag agcgcgtcgc ccggcttcta cgaggtcgcg ctcgacacgg
     8941 gcgtgcgtgt gagcctctcg gccaccgaac gcacaggctt cggcgtcttc acctacccgg
     9001 attcgcaggc cgccacgctc ctgttcgatg tcgggcgcaa tgcctccggc gtgacggaag
     9061 gcacatggca gcgagacggc gaccggcgcg tgagcggttc ggtcacgagc ggcggcttct
     9121 gcggcacgcg caacaagtac aagctctact tcgcggcgga gttcgaccgc ccgtttgtga
     9181 cgaccgggac gtgggaggga gaacgaatct cacacggcgg tcgctccgtg aagggcgcga
     9241 aagtcggggg cttcgtcatg ttcgatccgg cgagcgagcg cgccgtgcgt gtgaaggtgg
     9301 gcgtctctta cgtgagcgcg gagaacgcgc ggctgaacct gcgcgcagag aacgccgggt
     9361 ggaacatcaa cgccgtccgc gcacgcgcac gcgcacgctg gaacgacgcg ctcagccgca
     9421 tcgagatcgg cggcgggacg ctcccccaac gtcaggtctt ctatacggcg ctttaccaca
     9481 cgctgctcca cccgaccatc ttcagcgacg ccaacggcga gtatctcggc ttcgacgata
     9541 aagttcacgc ttcgcgcacg cacacgcaat acacgaactt ctccggctgg gacatctacc
     9601 gctcgcagat tccgctcgtc gcctttctct tccccgaaga ggcgagcgac atgatgcagt
     9661 cgctcgtcaa cgcggcgcag gagggcggcg gacttcccat ctggccggtc gccaacgacg
     9721 agagcggcgc gatgtacggc gacccggcga ccgcgagcat cgcgagcgcg tacgccttcg
     9781 gcgcgcgcaa cttcgacacg cgcgccgcgc tcgccgcgat gatcaagaac gcgaccgacc
     9841 ccgaaacgaa gtccagaact tacccggcgc gtcccgccct cgcagactat ctcaagtacg
     9901 gctacatccc gatggaacag aaggggctgc gcggctcgcc ctccgtcgcg ctcgaatacc
     9961 agaccgccga cttcgcgctc gcgcagttcg cacgcgcgca cggcgacacg gccacccacc
    10021 gcgagatgat gcgccgcgcg caactctgga cgcgcctgtt cgaccccgtc aacaagtaca
    10081 tacgcggtcg ctggcgcgac ggctcatggc tgccgggctt cgacttcaag gagatgctct
    10141 ataaaccgga actgccgtgg cgaccggaaa gccacaagtc ttacgtcgag ggcaacgccg
    10201 tgcagtacac ctggatgatc ccgcacaaca tgcgcacgct cttcgaccgc atcggcggaa
    10261 acgacgtggt cgtgcgcaag ctcgacgatt tcttcaaaga gttcaacgcc ggaccagacc
    10321 gcccctactt cttcatcggc aacgagccgg tcttccccgt gccctgggct tacaactacg
    10381 cggggcagcc ctggaagacg caacagatca cgcgccgcgt gctgaccgaa ctcttcacca
    10441 acacgcccgg cggccttccc ggcaacgacg acctcggcgc aacctcttcg tggatcgtct
    10501 tcgccgcaat cggcatgtac ccggtgacgc ccggcgtcgg cggcttctct ctcaacagcc
    10561 cgctcttccc ggagatcaag atcaggatgg gaggcagggg cggcaagtcg ctcaggatcg
    10621 tcggcgacgg agcctccacc ggcacgcctt acgtgcagga actacgtctc gacggcaagc
    10681 cccacgagag cacatggctc cccttcgagc gcatctcaag gggcgcgacg cttcgcttca
    10741 aactcggcgc gacgccgaat acgcagtggg cgactgcgcc cgccgccgcc cctccctcct
    10801 tccccgaagg catggagagc gcgagcgcgc cggggcaatg agcaggatgc ggctcgcccg
    10861 cacgactcga taaactgtga aggcgggaca gcactactgt ctaaactgtg aaggcgggac
    10921 ggtgaggccg tcccgccttt cgtgtaccgg cgaccgcttc ttcaccgccg gacgtgaaag
    10981 tcatcgcttt aaagtcatcg cttcaaagct atcgcctcaa gttcatctct tctcgtcggc
    11041 tagtttcaag acgctcgcct tccagtcggc agccgtgagc gtcctgagtt gcggcaactc
    11101 ggcggcggcg aagacgagcc ttctgagcga cgggatgata gtgaactcaa cttcgcgcgg
    11161 catcttggca atcctctcaa gcagcaccag tttctcttcg cgccagcccg ccgggggcgc
    11221 gatgcccgcc gataaatact gcaccgcctc caacccggcc tcgctcatct ccgataaatt
    11281 tccggcgagc ggttcggcat cgcgcatcag cggcgagcgg tcggccatca cccgcaccgg
    11341 agcatagacg ccgcgccacc cgtccagaaa cccgcgcacg cgttcacgcc gcgactcgaa
    11401 gcgcggcgca tcttctaaga gcgcgcccac ggctgaggcg aactcgcgcg ccgcgcggct
    11461 gtcgggcgca acggcgtcaa cgagacgcgt cagcggcgtc atctgcgtga tggggcgcac
    11521 gtgttcgcgc gcgaagaact tcggagccag cgtgcctgtc aatgtctcca gcggttccac
    11581 acgctcgctc ccggccagtc gccgcagcat cttcccggca taggttcgat gcgtcagacc
    11641 aaactcttcg agttggacgg cgacagcttc gagccttcga tacatgtcct ccacgtctct
    11701 cgtctcgcgc ggcgaccaga gac
//
LOCUS       DASWNS010000463        26540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_122089, whole genome shotgun sequence.
ACCESSION   DASWNS010000463 DASWNS010000000
VERSION     DASWNS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 26540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122089"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VGB76_15875"
     CDS             <1..412
                     /locus_tag="VGB76_15875"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0100423.1"
                     /translation="PDGRTLLVGESNHNRILSYDIHAPGKAGKRRVFANLPTKAGEQI
                     ANEPDGICLDVAGNLYVAHYGMRQVQVLSPNGRLLRRYAAGNLTTSNVAFGGARMDQL
                     YVTGALADEKTSRGGLFRLDLKGVRGLKTVTSDK"
     gene            588..1958
                     /gene="glmM"
                     /locus_tag="VGB76_15880"
     CDS             588..1958
                     /gene="glmM"
                     /locus_tag="VGB76_15880"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY0100424.1"
                     /translation="MRAIPTLKISISGVRGVVGESLTPTLLVRFAQAFGTYTGPGTIV
                     IGRDTRTSGEMVRQAVVAGLLSSGCRVVDLDICPVPTVQLLVREQRARGGIAITASHN
                     PAEWNALKFINSAGLFLADAQARQLLDIYHQGEYRKVAGAEMRAVEPSAGAIDLHIKA
                     ILDALGALPARPTGARKLRVAVDSCNGAGSIVAPRLLDALGVEVVPLNVTPDGLFPRG
                     AEPIEENLDALAAAVREHDCDVGFAQDMDADRLAIVSERGDVIGEEYTLVLAARYVLS
                     RERGAVVANLSTTSALDAVAARFDCPVYRSKIGEANVAEEMLRRGAVVGGEGNGGVIY
                     PRINFARDSLVGMALILHLLAETRRTISELVAELPRSYMVKEKLACRSDAARVALRMV
                     RDVYAQYAMDLRDGVKVTTPDGWFLVRGSNTEPIIRIVAEAADRERAWRMIEDVRTRV
                     LARLAP"
     gene            2048..3190
                     /locus_tag="VGB76_15885"
     CDS             2048..3190
                     /locus_tag="VGB76_15885"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEY0100425.1"
                     /translation="MDTQAEEPGGREAAAGRLLSLDVFRGLTIAGMILVNNPGSWEAI
                     HPPLEHAAWHGWTPTDLVFPFFLFIVGVSIALALARRAEAGGTKRDLYLKIVRRTLII
                     FALGLVLAGFPRYELSTLRIPGVLQRIAVCYLVASVIFLNTKWRAQALIAASLLLLYW
                     ALMLSIPVPGYGAGDLSMEGNLAAYLDRALLGRHTWKPLYDPEGILSTIPAIATALCG
                     VLTGHWLRTRRSDLDKVAGMFVAGASGVLAGWAWGGWFPVNKALWTSSYVLLTAGLAL
                     QFLAVCYWLVDIKGVRRPAKPFLIFGSNALIIYFLAELFSRIISLITLPRADGGRVDL
                     KTYIYENAFASWASPENASLMVAVCIVLLWLGVATLLYRRRIFIKV"
     gene            3354..3911
                     /locus_tag="VGB76_15890"
     CDS             3354..3911
                     /locus_tag="VGB76_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100426.1"
                     /translation="MGAFGKWLMHDDQKGLWEILFACVLNIVLLVLVGLLLWPLGKAM
                     LAFRLAQGYGILWLVIDVTGLLVILLQKFFGVNLYDHADAYVNSNLAVSCFLQTGWSA
                     FAALTLQHFVAGASIWIASSLYLVGVLSCLVAFYAVSSFYQGHIYRLISLPLALISFV
                     VFSVWPAGGRLLYGWFFNLFGAYAP"
     gene            3946..4392
                     /locus_tag="VGB76_15895"
     CDS             3946..4392
                     /locus_tag="VGB76_15895"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0100427.1"
                     /translation="MTNDNSKATDEAQIRAFIEERIKAVNDREIERLMSQHAPDVLMF
                     DVLDPLRYIGAEKVRERAAQWFSRYRGSIGYEVRDLNIETGETAAFCHYLYHVSGTMT
                     NGREVGMWVRSTVCFRKVDDEWIVAHEHTSVPFDAESGKASVDLKP"
     gene            4408..4596
                     /locus_tag="VGB76_15900"
     CDS             4408..4596
                     /locus_tag="VGB76_15900"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0100428.1"
                     /translation="MKTVRWAGAWLIRAPRNEVYALMTDFERWPETLPGIVEQARVIS
                     RTDTAVVLEGDFNLQIDK"
     gene            4680..5420
                     /locus_tag="VGB76_15905"
     CDS             4680..5420
                     /locus_tag="VGB76_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0100429.1"
                     /translation="MMSTEVEQMNGGRGKAAQARKARGERTRQTILEVAVDVASAEGL
                     EGLTIGRLAAELSMSKSGLFAHFGSKEELQLATVEAAREIFIREVIRPSFASSPEGLP
                     RLWKLCDVWLAYVKGEVFRGGCFFAAAAAEFDGRPGLVRDRVAGIMKEWLATLRGAIA
                     EAREAGQLKEEADSAQLAFELNALEMGANWAFQLYGDRLAFTRARDAILERLRRYSTA
                     SGARLLPPAGKGGGVRRAPRAKRGESRT"
     gene            5417..6322
                     /locus_tag="VGB76_15910"
     CDS             5417..6322
                     /locus_tag="VGB76_15910"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0100430.1"
                     /translation="MNAPEPHHSTPLIFERQASWEGIRLAHYRFHAGVVPEHSHRQHL
                     VLVALTRDCRSELRTPGGFNARPPVSGGVCVIPSGQPFAATLEGEAEFLAVYLDPSLV
                     MRAAEDARNRAGGGVEVIVKTSINDPLVIGIGQALLAELETDAPGGRLYAESLANVLA
                     VHLLRHYTAAGGGDMRRFIGGLSGQRLRRVLAFVADNYERDLSLDDLAGEAGMSTFHF
                     AREFKRATGTTPHQHLIKFRVEHAKALLAEGEMPLAEVGLRSGFSHQSHFTRLFRKLT
                     GTTPQSYRLMIQPTTRRVISDLPNG"
     gene            6428..7162
                     /locus_tag="VGB76_15915"
     CDS             6428..7162
                     /locus_tag="VGB76_15915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100431.1"
                     /translation="MWTKRLLLAFVLLSLPVVTATGQESRSEARERIRRGNEMYARAE
                     YEAAIREYGRVAREEGEAYAQSLYNIGVCQYELWRTGEAIEMYRSAVAASGGRYPKAS
                     YALGVALEDSRQWAEAREAYRQTLAASGGNYREAELAVAHYRLGLLAMREGDYESASA
                     LFREAIARSRRRFPASHNNLGVSLALAGRLELAEREFEAALRLSRGAFSEASHNLRLC
                     RAARASGTITEFASLKMVEAIAATIE"
     gene            7522..9348
                     /locus_tag="VGB76_15920"
     CDS             7522..9348
                     /locus_tag="VGB76_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100432.1"
                     /translation="MNETESLPFNELTTERPASPRDEYTRRRTAHLAEASRQMRRFRT
                     VGILRLAMLVAGLAFGWLVYTGLAAWWLLLPPVVGFLILADMQQRITHGRRRCERAAA
                     LYEQGLARLDDRWAGTGATGERFIFATHPYAEDLDLFGHGSLFELLSRARTRVGEETL
                     ANWLLAPAAPHILRARQAAIVELRPRLDLREDLALLGEGVRSGKDAQTLAEWAAAPAW
                     PISRLTRLTALALALLALVTLILWIAGFGVLPFLVALVLGRVYAFRLGNTVERVIEAV
                     EEPGRDLTLLSEVLSRLEREQFESPRLVEVRAALDVEGLSPSQQIARLNRLIDLLDAR
                     RNMIFAPLAFVLLWPLQFAIAIERWRQSSGVAVARWLAAVGEFEALGSLAGYSYEHPD
                     DPFPELLDGAARFEGEGLGHPLIPEARSVRTDMRLSDDLRVLIVSGSNMSGKSTLLRT
                     LGTNTVLALAGAPVRARALRLTPLQLGASIRVQDSLQAGASRFYAEITRLRQIVQLTE
                     GTLPVFFLLDEILHGTNSHDRRIGAEGVVRGLIERRAIGLITTHDLALARIAEDLAPR
                     AANVHFQDHLEEGKMTFDYRLRPGTVQRSNALELMRSVGLDV"
     gene            9681..10925
                     /locus_tag="VGB76_15925"
     CDS             9681..10925
                     /locus_tag="VGB76_15925"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY0100433.1"
                     /translation="MVKRPGIFPTFFLSGFECSTFLWKDKQRRNLIEETQHHKHVRED
                     YNILHDLGIAVAREGIPWPLIDRGGQYDFSPIDPMIEAMNQAQVIPIWDLCHYGYPDD
                     LDPFSEEFTKRFADYCRAAAQYVVPRLRAPYFFTPINEITFFSYCGGEWGWVAPYRTS
                     REDRYKLRLALCRAAIAGVKAIREVEPTARMVHIDPLVQVVAPRDRPDQVEAANQETY
                     VDTFLAWDIIGGKAHPELGGSPEILDIVGANNYSFGQMEYREQGPHAALDPGDERIKP
                     LCDLLSTVWERYRRPMIIGETSGLGHGRAAWLKDVMEESLAAVNLGMDLQGICLFPAV
                     DMPDWHTGKWLNNGICDLLDEQGDLRRVPHDPYVQELRRWQRELNRVSVLDADPFSDP
                     VELQDVVEAAARLQKKPDQDWS"
     gene            11164..11685
                     /locus_tag="VGB76_15930"
     CDS             11164..11685
                     /locus_tag="VGB76_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100434.1"
                     /translation="MRRILSVLLCCWLLQFAGGTIASGQQIPVAPLAEDASLAETLKW
                     LESQLVRHGKMRYYDPFGPFEHKYYDTRFEYLRADGCVVRYRRSTMRRGDEYSVNLSD
                     LDPAQVKAEVPKNWKGGRVIFSSATSSPAVTYHAKGGKARSHPTGEFASNDKAALEGI
                     AGALRRAIASCRR"
     gene            11730..12428
                     /locus_tag="VGB76_15935"
     CDS             11730..12428
                     /locus_tag="VGB76_15935"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0100435.1"
                     /translation="MSRSGQTLESLQSDFDRIALLSDESWNHNSHYHAYLIAHIPEQC
                     RQILEIGCGTGEFSRLLAPRAEKVLAIDLSPQMIRLAQERAKLYPNIDFVAGDVMACQ
                     FAENQFDCIATVTTLHHLPLETILRKIRAALKPGGVFLCLDLYQRSNPTDLLFDVMAY
                     PANLLLRLIKTGTPRASGAVGEAYAEHGKTDVYLTLPQIEQICAATLPGASVRRHLFW
                     RYSIIWKKEVAGQS"
     gene            complement(12534..13769)
                     /locus_tag="VGB76_15940"
     CDS             complement(12534..13769)
                     /locus_tag="VGB76_15940"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0100436.1"
                     /translation="MEVKPLRRMIFALVFFALLAPPCAPGQSSPGTSGDFARAYQEFR
                     TNYLSELRKERFVGSSFYLVRDNRVVAREFYGLADAGRSVPVDEQTIYHWASITKTFT
                     GVAIMQLRDRGLLKLDDPVVKYIPELRAAHNPFGDMAEITIRHLMTHSAGFRNPTWTW
                     RDDGKDWQPFEPPGWTQLAAMMPYTEVLFRPGSRFRYSNPGVIYLGRIIELLTREDYE
                     VYIDKNIFKPLEMYHSYFDSTPPHLLKHRSHSYYVRDGARKEARFDADTGITVSNGGL
                     NAPLPDMVRYVNFLLGDPSKQAAYDGVLKRASLEEMWRPQLVASDDFTQGWMRAETAV
                     GLSFFVDEIGGRRYAGHNGDQNGFKVYMSLCPSTRTASLLAFNTETRPTMTGTGNLLA
                     PESKVARSVLNLFQSIPAR"
     gene            complement(14002..15018)
                     /locus_tag="VGB76_15945"
     CDS             complement(14002..15018)
                     /locus_tag="VGB76_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY0100437.1"
                     /translation="MIELFPLADYWWFYLGFTGFVLLLLALDLGVFHREAHEVSFREA
                     ATWSMVWVALALLFNFLLYQYALWKFPQDPRLLAMPGFDPAGAAWRVSLEFLTGYIVE
                     KSLSVDNVFVFVLVFGYFAIPAKYQHRVLFFGIIGALIFRAIFIALGSALMQYHWVVV
                     VFGAFLIFTGIKMMFAPEKGVEPEKNLLIRLFRRFVPTTSELHGQRFFVRHKGVLYAT
                     PLFVALLFLEATDVIFAVDSVPAIFALTSEPLIVFTSNIFAILGLRALYFMLAGAIDK
                     FHMLKYGLALVLIFVGLKMVWLNDMFGGKFPISLSLGIIAAVIVSSVVLSLMFPKAHP
                     LPEV"
     gene            complement(15371..16141)
                     /locus_tag="VGB76_15950"
     CDS             complement(15371..16141)
                     /locus_tag="VGB76_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100438.1"
                     /translation="MMRLVRQHLPVALFVALFPASLLIVNACAAPQQGGANVQQASVN
                     QRAMNHNETPDASTNAASPDTTHGETPAPDANTLPAGADDLSFETTIRATAKDQLLIS
                     YTVRNRGRQPMLLMNRPPARGGASGSQPNPNFVYVETQSDGVVEISKRVKEPPRETTV
                     FVPDVPGATLLAPGQSFSEEVRVDLAARRRKSAQPVRDVPDIPDPVKRVRFCLGVAPS
                     EGITTRAFGQGKNRTIYPDTGAVLLKQRVLCGETTELQ"
     gene            complement(16228..18057)
                     /locus_tag="VGB76_15955"
     CDS             complement(16228..18057)
                     /locus_tag="VGB76_15955"
                     /inference="COORDINATES: protein motif:HMM:NF026241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M91 family zinc metallopeptidase"
                     /protein_id="HEY0100439.1"
                     /translation="MGLAEIGQQLANQVSEAVQAGIERAREAVARIAQPGVSTEPQDE
                     NAMQLVSNRAVTSSTNSQTPASTLPGQQTELDARVQLARTNAQVNAAGGGGSSAASSA
                     TSETVIVSTKEVTIKRTSTPAADPTEPPIERIVVDAGGGDDQIRVTRDARTGATVINV
                     NGERHTISTSTYTDPADGREKAQVNVDGKPQQLGRNDPYLVTIRAGEGNDTLTVSQNV
                     RLNFTLEGGAGNDRIQGGAGNDRIEAGDGDDTVNGGGGRDYINGSRGNDKLYGSAGHD
                     TVYGGDGNDYIQGNAGEDYLEGGRGQDHVLGGAGHDVLSGGLDNDTLEGGHGNDKLYA
                     GEGTDRLYGNTAANRNPERATGRDTIYSQRGDTVGRDTGTTVVNVELAGQPGSRSVRI
                     EGSPEFVERVEQDIEFLRSSPTGRQMLTEFDKAYDSSRDPKSSWWLVGRFFNDGNSVT
                     IRELSEEDNGFAAPTDYTKTSLDPKTGQPGDGSDVIISYNTQLNQFPFRDPKNPADAW
                     RDLDPVVVLYHEMAHAYDDVYGVMQPYAQYQAKNPADNDPRDIGMQNRERQAVGLEND
                     GIPYDFDGDPKTPKSTANPHALTENGLRDELGRPRRPRYNIAP"
     gene            complement(18241..20085)
                     /locus_tag="VGB76_15960"
     CDS             complement(18241..20085)
                     /locus_tag="VGB76_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100440.1"
                     /translation="MTYKVDGGGGVQEIAYRQPEIVQQPEEKPEPIVEFATGKIWDAF
                     FGASALKERVQTNAQQQNPNVPQTFVNNGSNIPRGTEPKPPGADATPAEAAAYIDFYR
                     TGANYPGAEGFMFARQLLEHQNDPQWIQGYFKALGNDRTAELLNQSLSYETFQNWTDR
                     GDINEFVGVVRNSFAALDKAGLLNQADMDNLVGRWAASGKFNPQVAIELFGKLDAKHE
                     NLKSIFFQAAARQAANPQTKDGVGKDLAAAATHVLGQTSSDNQVSQLNLLRRAGGLEQ
                     FVKRATAGERTVSTLESMAAYYDRPGTTGANNQVEYGRIESLLYSLAYNDVRDGWDHR
                     PVSVTGDELHKLRTDFFNAAAQSLTDKKTQDNFRSNVMFKDGMSAVFMAEYDNIIKSG
                     LGTNGAGFDTLKFQPGLEKFFQQALFTPPLGYSSGSLANFLSNKVSEIGAALMDDSPG
                     AEQRWRDTHGGQSRMDASAVAGGLLGMLTNGLKSSLDQMKKDAEAQAQTVKLFVDIAF
                     GFIPGIGSKLGSSAAETFLKNVIGPIDGKIRDEIKNGAVEKAKELVMNQFKNGLRDLK
                     PDEMVLALFNSLNQTIPNGDNRGEQNFRAEFTSSYSTIINSPNRIVSQ"
     gene            complement(20225..21532)
                     /gene="sctN"
                     /locus_tag="VGB76_15965"
     CDS             complement(20225..21532)
                     /gene="sctN"
                     /locus_tag="VGB76_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HEY0100441.1"
                     /translation="MSVNLAEYIEAVDWLSPVEVRGRVTELVGLLIRASVPGARVGEL
                     CLIRSPHRARELRAEVVGFRGSEVILMPLGDIQDVAMGAEVVSTGASLKVRVGDGLLG
                     RVLDGLGEPADGKGAIEGGQERPVTARPPDALRRQRVTKMLATGVRSLDGVLTIGEGQ
                     RVGIYAAAGVGKSTLLGMLARNTEADVNVIALVGERGREVRDFIEQDLGEEGMRRSVV
                     IAATSNEPSLVRMKAAYVATAVAEHFRDQGKRVLLMMDSVTRFARALREVGLAAGEPP
                     ARAGFPPSVFSELPKLLERTGNSAHGSITAFYTVLVEGDDMTEPIADETRSILDGHIV
                     LSRALAAAGHYPAIDINQSVSRVMSAVARPPHVEAARRLREVLATYEAQKDLILIGAY
                     KKGSDPRTDYALGKLDSVNAFLRQGTAENAPPGVAISQLQRMF"
     gene            complement(21690..22331)
                     /gene="sctL"
                     /locus_tag="VGB76_15970"
     CDS             complement(21690..22331)
                     /gene="sctL"
                     /locus_tag="VGB76_15970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system stator protein SctL"
                     /protein_id="HEY0100442.1"
                     /translation="MSAGKVIKGAATGAQRDFKARGVVKRPVVEAQAEARLIIAAAER
                     EAAALRASAEAAALELRDAAYREGLEAALAELNEHLLDARDRRDNALAEAERDLLRLA
                     VKLAEKIIGREIAQDDATLAEIVAAALRNARQHEMLTVRVNPADLPAVQAHRARLDPT
                     GRARFLDLVADPRVTHGGCVIESESGTIDAQLDVQLGVLERALLARADGDNRP"
     gene            complement(22328..23365)
                     /locus_tag="VGB76_15975"
     CDS             complement(22328..23365)
                     /locus_tag="VGB76_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100443.1"
                     /translation="MAQPVEPERIDTLLALSIAFGQSAFESDEHAHDAGDANATTAYG
                     LFDALNEAARQSLERRAQWYAKLAHDKQAHWLTRTLARANHSRTQLDEHVHPSHILEA
                     LRDEPPRVRALILRYLPPSFADACTGALQAEAWDGAEPPAQLVGLVRKVFLSNFVAPG
                     ELRNPSPLETLTGVELARLVRLLGVHETAVACRGIGRAEAVGSFLRRFSAENARAILA
                     HMATLLKLESARVTLAEGLVHEALEQQQEPEAMLDWLGMHVLAASLAARESSAERAAY
                     VAQKLPFEAARALRRMVADCSVRYEHEVLRVIGREAESLALSLRHVKTAGGATKEAVV
                     NMQEEAFEVRT"
     gene            complement(23441..24400)
                     /locus_tag="VGB76_15980"
     CDS             complement(23441..24400)
                     /locus_tag="VGB76_15980"
                     /inference="COORDINATES: protein motif:HMM:NF013664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100444.1"
                     /translation="MRQIHRLADFRALSQRVIFGILLALVAVLTSACGEKSVARVASE
                     SEAIEIIDRLREYQIGANKEEVGEGEAAQWSINIDEGWFGGDESARATQVLRYYGLPR
                     PDELKLAKDDGGVFPSPAVENAQRLRVREIEMERKLRLLPGVARANVTIVLPEDDTIK
                     LDPYPATASVLLVHKEPQPTFTGEQVQNQVAGGVPGLKPEKVTVSMLFETPPPLRPQE
                     NVRRRNSLLLTIGAGLLTIFCFLLVILLLQTRRQRAEIAVLREGEEATIAVQEASDEQ
                     PAMLPAGASPSLRETTTTARQLPDATLTANRAGAIKEDPTSVK"
     gene            24659..25387
                     /locus_tag="VGB76_15985"
     CDS             24659..25387
                     /locus_tag="VGB76_15985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0100445.1"
                     /translation="MHDFAATTSLADRDQLIEQHRSYVRALAIKTMQAMAARVDLQEL
                     IAYGDVGLIEAAERFDPRRGVAFATFAHYRIKGAIYDGLREMGVFTRQHSSRSHFAAN
                     ANDLLQSGADDEQAALAEGGARAIESEIATTQSLIDALIPAYLLSLDSDAMPEIMDQQ
                     ALSMEQIEQRELIGFVLHLVRELPEDEQQLLDALYFKHRSMTELAAEMGISKSWISRL
                     HSRAIKHLRDRMQERGMLEADSPS"
     gene            25566..>26540
                     /locus_tag="VGB76_15990"
     CDS             25566..>26540
                     /locus_tag="VGB76_15990"
                     /inference="COORDINATES: protein motif:HMM:NF019123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1521 domain-containing protein"
                     /protein_id="HEY0100446.1"
                     /translation="MSNVSLLQSQSINFNFNFNGLFGAGANSELLALLEATSDLADAL
                     GNFNDKFAALAEKFGSTSALCQCAPVAPPVLEDSCHPAGSLRAEGDTITTPGGYKIEM
                     LSQYEWKITGPDGKETRVWGDPHVDEGDGGKWDFKRDSTFVLGDGTRINVNTVPYGDM
                     TVTGSLEIISGNERVLVSDIDQGKGKIGTVTQDGYQHANSFGDNDVFVMGRETDDWSF
                     RGREITGSENGGESFKTGGELHPLTTQVNRHGNPRDWANSIFDSLLDSWRGDLRTNDL
                     GYNAYCDRDNSGRVENDERDDRRQHAEALRDAFQALADMFDALSRWLTL"
BASE COUNT         4990 a         8188 c         8158 g         5204 t
ORIGIN      
        1 ccccgacggg cgcacgctcc ttgtaggcga gagcaatcac aatcgcattt tgagctacga
       61 catacacgcg ccgggcaagg cggggaagcg gcgcgtcttc gcaaacctcc ccacgaaagc
      121 gggcgaacag atcgccaacg aaccggacgg catctgcctc gacgtggccg gaaatctcta
      181 cgtcgcgcat tacggcatgc gacaggtgca agtgctgagt cccaacgggc gactcctgcg
      241 ccgttacgcg gcgggaaatc tcaccacgag caacgtcgcc ttcggcggcg cgcgcatgga
      301 ccaactctac gtgacgggcg cactcgccga cgagaagacc agtcgcggcg gcctcttccg
      361 cctcgacctc aaaggcgtac ggggcttaaa aacagtgacg agtgacaagt gacgagtgac
      421 gagcgagaac cagataaaag tgctgccgtc gttgtgactt tcatgtgcga caagtgatga
      481 gaaggaagac aagtttaaag acagtgtcgc tgttttaact tctcacctat cactcaacac
      541 tgtcttaact cgtcgcttgt gacctggcac tcgtcactgc tttcattatg cgagctatac
      601 cgactcttaa aatcagcatt tcgggcgtgc gcggcgtcgt cggcgagtct ctgacgccga
      661 cgctgctcgt gcgctttgcg caggcgttcg ggacgtatac gggacccgga acgattgtca
      721 tcgggcgcga cacgcgtacg tcgggcgaga tggtgcggca ggcggttgtg gcgggtctgt
      781 tgtcgagcgg ttgccgcgtc gtcgatctgg acatctgccc tgtgccgacc gtgcaactgc
      841 tcgtgcgcga gcaacgcgcg cgcggcggca tcgccatcac ggccagccac aacccggctg
      901 agtggaacgc gttgaagttc atcaactccg ccggactctt cctcgccgat gcgcaggcgc
      961 gccaacttct agacatctat catcagggcg aataccgcaa ggtggcgggc gcggagatgc
     1021 gcgccgtaga gccttcggcg ggcgcgattg atcttcacat caaagccatc ctcgacgcgc
     1081 tcggcgcgct ccccgcgagg ccgacgggcg cgcgcaaact gcgggtcgcc gtggactcct
     1141 gcaacggggc ggggtcaatc gtcgcgccgc gcctgctcga tgcgctcggc gtcgaagtcg
     1201 tgccgctgaa cgtgacgccg gacggcctgt ttccgcgcgg cgcagagcct atcgaagaga
     1261 acctcgacgc gctcgcggcg gcggtgcgcg aacatgactg cgacgtgggc ttcgcgcagg
     1321 acatggacgc agaccgcctc gccatcgtct ccgagcgcgg cgatgtgatc ggcgaggagt
     1381 acacgctcgt actcgcggcg cgctacgtcc tcagtcgtga acgcggggcg gtcgtggcaa
     1441 acctctcgac gacgagtgcg ctcgatgctg tggccgcgcg cttcgattgt cccgtctatc
     1501 gctcgaagat cggcgaggcc aacgtggcgg aggagatgtt gcggcgcggc gcggtcgtcg
     1561 gaggcgaggg caacggcggt gtcatctacc cgcgtatcaa cttcgcgcgc gacagcctcg
     1621 tcggcatggc gctcatcctg cacctgctcg ccgagactag acgcaccatc tctgaactcg
     1681 tggccgaact cccgcgttcc tacatggtca aggagaaact cgcctgccgc tcggacgccg
     1741 cgcgtgtcgc gctcagaatg gtgagagacg tatacgcgca gtatgcgatg gacctgcgcg
     1801 acggcgtgaa ggtgacgacg cccgacggct ggtttctcgt gcgcggctca aacaccgaac
     1861 cgatcatccg tatcgtcgcc gaagccgcag accgcgagcg ggcatggcgc atgatcgaag
     1921 acgtgagaac acgcgtgctc gcgcgcctcg ctccatagag tttgaaattt tcaaactcca
     1981 actcatcttt tcctttgcgc ttttgcgcct gaacgcagtt taagcgaagc cgtttggaga
     2041 gcaatgtatg gacacgcagg cggaagagcc gggagggcga gaggcggcgg cggggcgtct
     2101 gctttcgctc gacgtgtttc gtggcctcac catcgcaggg atgatcctcg tcaacaatcc
     2161 cggcagttgg gaagcgattc atccgccgct cgaacacgcc gcatggcacg gttggacgcc
     2221 gaccgatctg gtcttcccgt tcttcctgtt tatcgtcggc gtctccatcg cgctcgcgct
     2281 cgcgcgccgc gccgaagcgg gcgggacgaa gcgcgacctg tatctcaaga tcgtccggcg
     2341 caccctgatc atcttcgcgc taggcctcgt gctcgcgggc tttccccgct acgaactctc
     2401 cacgctgcgc atccccggcg tcctccaacg cattgccgtc tgctacctcg tcgcctccgt
     2461 catcttcctc aatacgaagt ggcgtgcgca ggcgttgatc gcggcgtcgc tcctgctgct
     2521 ctactgggcg ttgatgctct cgatccctgt gcccggctac ggcgcgggcg atttgagcat
     2581 ggaaggaaac ctcgcggcct acctcgaccg cgcgctgctc gggcgtcata catggaagcc
     2641 gctctacgac ccggaaggca tcctcagcac gatccccgcg attgccaccg cgctctgcgg
     2701 cgtgctcaca ggccactggc tgcgcacgcg ccggagcgat ctcgataaag tggctggcat
     2761 gttcgtcgcg ggcgcgtcgg gcgtgttggc gggctgggcg tggggcggct ggtttcccgt
     2821 caacaaggct ctctggacga gttcatacgt tctactaacc gccggactcg cgctgcaatt
     2881 cctcgccgtc tgttactggc tcgtggacat caagggcgtg cgccgccccg caaagccctt
     2941 cctcatcttc ggctccaacg cgctcatcat ctactttctc gcggaacttt tctcgcgcat
     3001 catctcgctc atcacgctcc cgcgcgccga cgggggcagg gtggatttaa agacgtacat
     3061 ctacgaaaac gctttcgctt cgtgggcgtc gcccgaaaac gcctcgctca tggttgccgt
     3121 ctgcatcgtg ctgctctggc tcggcgtggc aacgctcctc tatcgcaggc gaattttcat
     3181 caaggtctaa aaaagctccg cgccggaagt tcgttactaa ataagagaga caatctcaac
     3241 caacgagtca cgccctcgcc agcgaacttc ttcaaccctt gaccgcttgg cagcacgtct
     3301 gtcaacgtcg cgtcatagat tctttaaaga gctagtccgt caggcacata tttatgggag
     3361 cgttcggcaa atggctcatg catgacgatc agaaaggact ctgggaaatt ctgttcgctt
     3421 gcgttctgaa catagttctc ctcgttctcg tcggactgct gctgtggcct ctgggcaaag
     3481 cgatgctggc gtttcgtctg gcgcagggct atggcatctt atggctcgtt attgatgtga
     3541 ccggcctgct ggttattctt ctgcaaaagt ttttcggcgt gaacctgtat gaccatgcgg
     3601 acgcttacgt aaactcgaat ctcgccgtca gttgcttttt gcagaccggt tggtcggcct
     3661 tcgccgcgct cacgttgcag cacttcgtcg cgggcgcgtc aatctggatc gcgtcgagcc
     3721 tgtatctcgt cggagtgctc tcgtgtctcg tcgccttcta tgccgtatct tctttctacc
     3781 aggggcacat ctacaggttg atcagcctgc ctctcgcctt gatcagcttc gtcgtgttca
     3841 gcgtgtggcc tgccggtggc cgcctgttat atggatggtt ctttaacctt ttcggcgcat
     3901 acgcgccctg accatgcggc gtgcgcttaa acaaacgaga gtgatatgac aaacgacaac
     3961 agcaaagcga ctgatgaggc acaaattcgc gcgttcatcg aggaacggat caaggccgtg
     4021 aacgacaggg agatcgaaag gctgatgtct caacacgcgc cggatgtctt gatgttcgac
     4081 gttctcgacc cgctgcgata catcggagca gagaaggttc gagaacgcgc cgcgcagtgg
     4141 ttctcccggt atcgagggtc aatcggctac gaggtgcgcg acctcaacat cgagacgggc
     4201 gagacggcgg ccttctgcca ttacctctat catgtcagcg ggacgatgac gaacgggcga
     4261 gaagtcggca tgtgggtgcg ctcgaccgtc tgcttccgca aggttgacga cgagtggata
     4321 gtcgcgcacg agcatacttc cgtgcccttc gacgcggaga gcggaaaagc gtcggttgat
     4381 ctcaagccgt agttaagggg ctgagagatg aagactgtgc gctgggcggg agcgtggctc
     4441 atccgcgccc cgcgaaatga agtttacgcc ttgatgacag actttgagcg ttggccggag
     4501 acgctgcccg gcatcgtgga acaggcgcgc gtcattagcc ggacggacac cgccgttgtc
     4561 ttggagggtg atttcaacct gcaaatcgac aaatgatcag gctcaggagc gtcgctcgta
     4621 aaccgtcgcg cggaaagtgt gtgcgaaaac gaacgttcgt gctatattga gcgcgagtta
     4681 tgatgagcac cgaagttgaa cagatgaacg gggggcgggg gaaagccgct caggcacgca
     4741 aggcgcgcgg cgagcgcacg cgtcagacga ttctcgaagt ggccgtggat gtggcgtcgg
     4801 cggaggggct tgaggggctg accatcgggc gtctggccgc cgaactctcg atgagcaaga
     4861 gcggcctgtt cgcacacttc ggctcgaaag aggagttgca actggcgacc gtcgaggcgg
     4921 cgcgcgagat ttttatacgc gaggtgatcc gcccgtcctt cgcgtcgtcg ccagagggac
     4981 tcccgcgtct ctggaagctg tgcgacgtgt ggctcgctta tgtgaagggc gaagtctttc
     5041 gcggaggctg ttttttcgcc gccgccgcag cggagtttga cggtcgcccc ggactcgtcc
     5101 gcgaccgcgt ggcagggatc atgaaagagt ggctcgccac gctccggggg gctatcgccg
     5161 aagcgcgtga agcggggcag ctaaaagagg aggcagactc cgcccaactc gccttcgaac
     5221 tgaacgcgct tgagatggga gccaactggg cttttcaact ttacggagac aggctggctt
     5281 tcacgcgtgc gcgcgatgcg atccttgaac gcctgcgccg ctactccacc gcgagcggcg
     5341 cgcgcctgtt gccgccagcc ggaaagggcg gaggcgttcg tcgtgcgccg cgtgcgaagc
     5401 gaggtgagag tcgaacatga acgcgccgga accgcatcat tcaactcctc tgatcttcga
     5461 gaggcaggcc agttgggagg ggatcagact cgcgcattat cgttttcacg cgggcgtcgt
     5521 gcccgaacac agccaccgtc aacatctcgt tctcgtcgcg ctcaccagag attgcagaag
     5581 cgaactgcgc acgccgggcg gattcaacgc gcgcccgccc gtgagcggcg gcgtgtgtgt
     5641 gattccttcg gggcagcctt ttgcggccac gctcgaaggc gaagccgagt ttctcgcggt
     5701 ctatctcgac ccgtcgctcg tcatgcgcgc cgccgaggat gcgcgcaaca gggcaggggg
     5761 cggggtagaa gtgatagtca agacctctat caacgaccct ctggtgatcg gcatcggtca
     5821 ggcgttgctc gccgagttgg agacggacgc tcccggtggg cgactctatg cggagtcgct
     5881 ggcgaacgtc ctcgccgttc acctcctgag gcattacacg gctgcgggag gcggcgacat
     5941 gcgacgcttc atcggcggcc tctccgggca gagactccgg cgtgtgctcg ccttcgtcgc
     6001 agacaactac gagcgcgacc tgagccttga cgatctcgcg ggcgaggccg gaatgagcac
     6061 cttccatttt gcgcgcgagt tcaagcgtgc gacgggcacg acgcctcatc agcacttgat
     6121 caagtttcgc gtggagcacg caaaggcgtt gctggccgaa ggcgaaatgc cactcgcgga
     6181 agtgggtctg cggtcgggat tcagccatca gagccacttc acgaggctct ttcgcaagtt
     6241 gacgggcacg acgccgcaat cctaccgcct catgatccaa cctaccaccc gacgcgtgat
     6301 ctccgacctg cctaacggat agcaagatta atcaaccgcc cgcaataaca ggaaagaccg
     6361 ggggcgcgcg ccgtccttag actcacttcc gtctttaatt ccaaacgaga cgaaggagtg
     6421 attcaagatg tggacgaaga gattattgct ggcgttcgtg ctgttgtctc tgccggttgt
     6481 tacggcgacg ggacaggaga gccgttcgga ggcgcgcgaa cgcatccggc gcggcaatga
     6541 aatgtacgcg agagccgagt atgaagcggc gatacgcgag tacgggcgcg tcgcgcgcga
     6601 ggaaggcgag gcgtacgcgc agtctctcta caacatagga gtctgtcagt acgaactgtg
     6661 gcgcacaggg gaagccatag agatgtaccg gagcgcggtg gcggcgagcg gggggcgtta
     6721 tccgaaggcg tcttacgcgc tcggcgtcgc gcttgaagat tcgcggcagt gggcggaggc
     6781 gcgagaggct taccgccaga cgctcgccgc atcgggcggc aattaccggg aagccgaact
     6841 cgcagtggca cactatcgtc tcggactgct cgccatgcgc gagggcgact acgagagtgc
     6901 gtccgctcta ttcagagaag ccatcgcgcg ctcccgccgc cgctttccgg cgagccacaa
     6961 caatctcggc gtgtcgctgg cattggcggg gcgtttggag ttggccgaaa gagagtttga
     7021 agctgcgctc cggctctcgc gcggagcttt tagcgaagcc tcacacaatc tcaggctttg
     7081 tcgcgcggcg cgtgcaagcg gaacgataac ggaattcgcg tcactgaaaa tggtggaagc
     7141 catcgccgcg acaatcgaat agttcaacac gacaatcgag cacacggctt cgcaggcggt
     7201 caggtgaagg cgcgcgtcaa cggcaggcgt tccgtgtcgg gcgcgttgga tgcacgcgtc
     7261 cggcgtgtgc gtttgtcgag ctaaaggatg cggcgttcgt cgtcggttac gacaacttgc
     7321 cgcgccggaa tccatgaaat atttattctt caataactat tgtacggggc gttccggttt
     7381 gagcaacggc atcatgtcca tcgaacccgg cgtttgctcc agaccgcccg gatgcgtgaa
     7441 tggcctgaga gctttatccg cgagcatcga gatagccggt cggctacgct aaacccggag
     7501 caaatggttt aagctgtagc catgaatgag acggagagtt tgcccttcaa cgaattgacg
     7561 acagagcgac ccgcgagtcc gcgtgatgaa tatacgcggc gacgcacggc gcacctcgcg
     7621 gaagcgtcgc ggcagatgcg acgttttcgc acggtcggaa tcttgcgcct cgccatgctc
     7681 gtcgccggac tggcgttcgg ctggctcgtc tacacagggc tggccgcatg gtggttgctg
     7741 cttccgcccg tcgtcgggtt cctcatactg gccgacatgc agcagcgcat cacacacggg
     7801 cgtcgtcgct gcgaacgtgc cgccgccctc tacgagcagg gacttgcgcg gctcgatgac
     7861 cgttgggctg ggacgggtgc gacgggcgag cgtttcatct ttgccacgca cccttacgcg
     7921 gaagatttag acctgttcgg tcatggctca ctcttcgaac tcctatcgag agcgcgtacc
     7981 cgcgtgggcg aagagacact ggcaaactgg ctgctcgccc ctgctgcgcc ccacatcttg
     8041 cgcgccagac aggcagcgat tgtcgaactt cgcccacgcc tcgacctgcg tgaagacctg
     8101 gcattgctcg gcgaaggcgt ccgttcaggc aaagacgcgc agaccctcgc ggagtgggct
     8161 gccgctcccg cgtggcctat ctctcgcctc acgcgcctga ccgcgctggc attggcattg
     8221 ctcgcgctcg tcacactcat actctggatc gcagggttcg gggtgctccc gtttctcgtc
     8281 gcgctcgtgc tggggagagt ctacgctttc cgtctgggga atacggttga acgtgtcatc
     8341 gaggccgtcg aagaaccggg acgcgatctg actttgctgt cggaggtgct cagccgcctt
     8401 gagcgcgagc aattcgagag tccgcgtctg gtcgaggtgc gcgccgcgct ggacgtggaa
     8461 ggtctgtctc cgtcgcagca gatcgcacgt ctcaatcgcc tgatcgatct gctcgacgca
     8521 cgccgcaaca tgatcttcgc gccgctggcc ttcgtcctgc tctggccgct ccagtttgct
     8581 attgccatcg aaagatggcg tcaaagctcc ggcgtggcgg tggctcgctg gctggccgcc
     8641 gtcggcgagt ttgaggcgct cggttcgctg gctggttact cgtatgagca cccggacgat
     8701 cctttccccg aattgctgga cggcgcggcg cgcttcgagg gcgaaggact cggccatcca
     8761 ttgatccccg aagcccgcag cgtacgcaca gacatgcgac tgtcggatga tctgcgcgtg
     8821 ctgatcgtga gcggttcgaa catgtccggc aagagcacgc tgttgcggac gctcggaacc
     8881 aacaccgtgc tcgcgctcgc cggtgcgccg gtgcgtgcgc gcgctttgcg cctcacgccg
     8941 ctacagttgg gagcgtccat tcgcgtgcag gactcgctac aggccggggc ttcccgtttc
     9001 tacgcggaga tcacgcgcct gcgccagatc gtgcaactga ccgaagggac gctccccgtc
     9061 ttcttcctgc tcgacgagat tctccacggc acaaactcac acgaccgccg catcggagcc
     9121 gaaggcgtag tgcgcggcct catcgaacgc cgcgcaatag gtttaatcac gacgcacgat
     9181 ctggcactcg cgcgcatcgc cgaagacctc gccccacgcg cggcaaacgt ccacttccaa
     9241 gaccatctcg aagagggaaa gatgaccttc gactatcgcc tgcgccccgg cacagtccag
     9301 agaagcaacg ccttggagtt gatgcgctca gtgggactcg acgtttagcc gacgccggga
     9361 cacgcggcct tgatgcccac tcaagaactc cgagttccgc gtcgagcgac acaacctcga
     9421 caaccaaaac ttaggagtcc gataaccaaa tttttccttt atgggcatct ccaataactc
     9481 atcttccaga tcaccccgtt gggcaaagtg cagaggtgtg acataaagct tatcggacgc
     9541 tgtctcggta aacaattcaa gttaacaaac tcatagcaac gcgacggctc gatagcgtgg
     9601 ccttccgacg taaggcctga cagttagccc ccacctgatt ctcgaacctg tttcgttaaa
     9661 tgttctgtta acatagggtc atggttaaac gtcccggcat tttcccgaca ttctttcttt
     9721 caggctttga gtgctcaact tttctctgga aagataaaca gcggcgcaat ctgatcgaag
     9781 agacacaaca tcacaagcac gtccgagagg attacaacat cctgcacgat ctcggtatcg
     9841 ccgtcgcacg cgaaggcata ccctggcctc tgatagaccg tggcgggcag tacgacttct
     9901 cgccgattga ccccatgatc gaggccatga atcaggcgca ggtgatcccc atctgggacc
     9961 tttgccatta cggttacccg gacgacctcg acccgttctc ggaagagttc acgaagcggt
    10021 tcgccgatta ttgccgcgcc gccgcgcagt atgtcgtgcc gcgcctgcgt gcgccatatt
    10081 tcttcacgcc gatcaatgag attacattct tctcctactg cggcggagag tgggggtggg
    10141 tcgctccgta caggacaagc agggaggatc gctacaagtt gcgtctggcg ttgtgcaggg
    10201 cggccatcgc cggagtgaaa gccatccgtg aggtagaacc gacggcgcgt atggttcaca
    10261 tagacccttt agtacaggtt gtagccccca gagacagacc ggatcaggtc gaggcggcta
    10321 atcaggagac ttacgttgac acctttctgg cctgggacat catcggcggg aaagcgcacc
    10381 ccgaactcgg cggctcgccc gagattctgg atattgtcgg ggctaacaat tactctttcg
    10441 gtcagatgga gtacagggag cagggaccgc acgcggcctt agaccccggc gacgagcgca
    10501 tcaagcctct ctgcgatcta ctaagcacgg tctgggaacg ataccgcagg ccgatgatca
    10561 taggggagac gagcgggctg ggtcatgggc gggcggcgtg gctcaaagat gtcatggaag
    10621 agtctctcgc ggctgtgaac ctggggatgg accttcaagg tatatgcctg ttccctgccg
    10681 tcgatatgcc cgactggcat accggcaagt ggttgaataa cgggatatgt gacctgttgg
    10741 acgagcaggg cgacttgcgg cgcgttccgc atgatccata cgttcaggag ttacgccgct
    10801 ggcagaggga gcttaatcgc gtgagcgtac tcgacgccga ccccttcagc gacccggtag
    10861 aattacaaga cgtagttgag gccgccgctc gccttcagaa aaagccagac caggattgga
    10921 gttagatacc cggactgcaa ctcgcggctg agtgctgcgg gaatcccgga tgatgcgctg
    10981 ctcgtcatca ttgaattgcc gggatacagg aacggtgtgc cgagagacaa ggtatctgtt
    11041 aatttctcag gggcgaccat agcggagggc aagccggttg aagatgagat acatacgggc
    11101 gaagaggttg tttgttgtgt cgccctgcgt gcgtgacatc cacccgtgga aaaggagttt
    11161 gagatgagac gtattcttag cgtgctgctc tgctgctggt tgctgcaatt tgccggaggc
    11221 actatcgcgt ccggtcagca gatacccgtt gctccactgg ccgaagacgc ctctctggca
    11281 gagacactca aatggcttga gtcccaactc gtgcgtcacg gcaagatgcg ctactacgat
    11341 ccatttggtc cgttcgagca taaatattac gacacgcgtt ttgagtacct gcgcgccgac
    11401 gggtgtgtcg ttcgttatcg ccggagcaca atgagacgcg gcgacgaata ttccgtcaac
    11461 ctgtcagact tagacccggc acaggtgaaa gcagaagtcc cgaaaaattg gaaaggcggc
    11521 agggtcatct tctcttccgc taccagcagt ccggcggtca cttaccatgc taagggaggc
    11581 aaggcgcgga gtcatcctac gggcgagttt gcgtctaacg acaaggcggc actggagggc
    11641 attgccgggg cgttacggcg ggcaatagcg tcgtgcagga gataacgcgt taggccgcaa
    11701 ggggctggat gctccggaac aaaaatactg tgagccgatc aggacagaca ctagaatcac
    11761 tccaatccga ttttgaccgg atcgccctgc tctccgacga aagctggaat cataactctc
    11821 attatcacgc atacttgata gctcatatcc ccgaacaatg ccgacaaatt cttgagatcg
    11881 gttgcggcac aggggaattc tctcgcctgc tggctccgcg cgcggaaaag gttctcgcca
    11941 tagacttatc tccgcagatg attcgtctcg ctcaggagcg cgcaaaactc tatccgaaca
    12001 ttgatttcgt agcgggcgat gtgatggcct gccagtttgc ggagaaccag tttgattgca
    12061 tcgcgaccgt tacgacactc catcatttac cgctcgaaac catcctgaga aaaataagag
    12121 ccgccttgaa gcccggaggc gtattcctct gcctcgacct atatcaacgc tctaatccga
    12181 ccgatctgct gtttgatgta atggcttatc ccgcgaattt acttctcagg ctgataaaaa
    12241 ctggaacgcc gagagcgtcc ggcgcagtag gcgaggcata cgccgagcat ggaaagacag
    12301 atgtatatct gacgttgccg caaatcgagc agatttgtgc cgctaccctg ccgggtgcgt
    12361 cggtgcgcag acacctgttc tggcgatact caatcatctg gaaaaaagag gtcgccggac
    12421 agtcgtaaac aaatcctctg gcaaatgact agcccgcaag gcgcaattga aggttccgtg
    12481 gcaagaagcg ctgcgtgggg gcgggaactg ttcgtcgagg ccgtgatgcc ccgtcacctc
    12541 gcggggatcg actggaagag gttcaacacg ctgcgggcta ccttcgactc cggcgcgagc
    12601 aggttgccgg tgcccgtcat ggtcgggcgc gtctcggtgt tgaaggccag cagactcgcc
    12661 gtgcgcgtcg aggggcacag gctcatgtag accttgaacc cattctggtc gccgttgtgc
    12721 ccggcgtagc gacgcccacc gatctcgtcc acgaagaagg agagtcccac cgccgtctct
    12781 gcgcgcatcc acccctgcgt gaaatcgtct gacgctacga gttgcggtcg ccacatctct
    12841 tcgagcgacg cacgcttgag cacgccgtca taggccgcct gcttcgatgg gtcgcccaac
    12901 aggaagttga cgtacctgac catgtcaggg agcggagcgt tcaggccacc gttcgagact
    12961 gtgatgcccg tgtcggcgtc gaagcgcgcc tccttcctcg ccccgtcgcg cacgtagtaa
    13021 ctgtgcgagc gatgcttgag caggtgaggg ggcgtcgagt cgaagtagct gtggtacatc
    13081 tccagcggct tgaagatgtt cttgtcgatg tagacttcgt agtcctcccg cgtcagcaac
    13141 tcgatgatac ggcctagata gatcacgccg gggttcgagt agcggaagcg gctgccgggt
    13201 ctgaacagca cttctgtgta gggcatcatt gccgcgagct gagtccatcc gggcggctcg
    13261 aacggctgcc agtccttgcc gtcgtcgcgc cacgtccaag tggggttgcg gaatccggcg
    13321 ctgtgggtca tcaggtgccg gatggtgatc tccgccatgt cgccgaaagg gttgtgcgcg
    13381 gctctcagtt cgggtatgta cttgacgaca gggtcgtcga gtttaagcag cccacggtcg
    13441 cggagttgca tgatggcgac gccggtgaag gtcttggtga ttgaagccca gtggtagatg
    13501 gtctgctcgt ccacgggcac gctgcgccct gcgtcggcga ggccgtagaa ctcacgcgcg
    13561 acgacccggt tgtcacggac gaggtagaag ctcgacccga cgaacctttc cttgcgcagt
    13621 tcgctcaggt agttagttct gaactcctga tacgcacgcg cgaagtcgcc gcttgtgccg
    13681 ggagaggact gcccgggggc gcacggcggg gcgagaaggg cgaagaaaac aagggcgaag
    13741 atcatacgac gaagtggctt gacttccatt agactctcac ctcaatgtct gttccgtgct
    13801 gtcgcataga ctcaagacgg ctcagtgcgc cgtcgcaaag aaatgttctt aaggcaacag
    13861 ggcgcgtcgt cgttcgagaa ctgcttactc ttcgacgttg ccgcgacgat tataccgacg
    13921 cggcagacgc cgccaagaat tttatgaccc gccccgtcat cattgggcgg ccttcaacgg
    13981 aatgtgccag cttcacttgc ctcagacttc cggcagcgga tgtgctttcg ggaacatgag
    14041 agaaagcacg accgacgaga cgatgaccgc cgcgataata cccagcgaca ggctgatcgg
    14101 gaacttgccc ccaaacatgt cgttaagcca gaccatcttt aacccgacga agatcagcac
    14161 caacgccagc ccgtacttca gcatgtgaaa cttatcaatc gctccggcaa gcatgaaata
    14221 gagcgcgcgc aagccgagaa tggcgaagat gttcgaagtg aacacgatga gcggttcgct
    14281 cgtcagagca aagatcgccg gaacggaatc caccgcgaag ataacatccg tggcctccag
    14341 aaagagcagc gcgacgaaca aaggcgtagc atagagcacg cccttgtgac gaacgaagaa
    14401 gcgctgcccg tgcaactcgg aggtggtcgg cacgaagcgt cggaacagtc ggatcagcag
    14461 gttcttctcc ggttcgacgc ccttctccgg cgcaaacatc attttaatac cggtgaagat
    14521 gagaaacgcg ccgaagacga ccactaccca gtgatactgc atcaacgcgc tcccgagcgc
    14581 gataaagatc gcgcggaaga tgagcgcgcc gatgatgccg aaaaaaagta cgcgatgctg
    14641 atacttcgcg ggaatcgcaa agtagccgaa gaccagcacg aagacgaaga cgttatcgac
    14701 ggagagcgac ttctcgacga tgtagccggt gaggaactcc agcgagacgc gccacgccgc
    14761 gcccgccggg tcgaagccgg gcatcgccag caagcgcggg tcttgcggaa acttccacag
    14821 cgcatactgg tagagcagaa agttaaagag tagcgcgagc gcaacccaga ccatgctcca
    14881 cgtcgccgct tcgcggaagg agacttcgtg cgcctccctg tgaaacactc cgaggtcgag
    14941 agccagcagg aggagcacga agccggtaaa gcccaaatag aaccaccaat aatcagccaa
    15001 cgggaacagt tcgatcatca ctcaatcctt tcacgccgaa catttatgac gagcgcgcaa
    15061 aagaaaagac ctcttgcgcg ctacatgagc cgcaaaaggt cttggtcatt gagcttcgat
    15121 caactcttcc cggtgcgccg gatgctccac agcgagcatc gtattggcgg catcaccaga
    15181 cccttgatag agcgggcgac ctactcccct ttcgagagcg acatactacg gagatggcat
    15241 aaaactgtca actcccaggc atgttaaatt tctgttacgc cccagcccga cgcgcggaat
    15301 cacctcgtga catgaagaga tggacatgcc gagtcttcac ccgtcgaaaa tatctttgca
    15361 aagtaattgt ctattgtagc tccgtcgtct caccacagag cacgcgttgt ttcaagagga
    15421 ctgcgcccgt gtccgggtag atggttctgt ttttaccctg cccgaaggcg cgcgtcgtga
    15481 tgccttcgga gggggcgacg ccgagacaga aacggacgcg cttaaccgga tcggggatgt
    15541 cgggcacgtc gcgcaccggc tgcgcgcttt tgcgcctccg cgcagcgaga tccacgcgca
    15601 cctcttcgct aaaactctgg ccgggcgcga gcagcgttgc gccgggcacg tccggcacga
    15661 acacggtcgt ctcgcgcggc ggctccttga ctcgcttact gatctcgaca acgccgtcgc
    15721 tctgcgtttc gacgtaaaca aaattagggt ttggctgcga cccgctcgct ccccctcgtg
    15781 cgggcgggcg attcatcaac agcatgggtt ggcgaccacg attcctgacc gtgtagctga
    15841 tgagcaactg gtctttcgcg gtcgcgcgga tggttgtctc aaaacttaga tcgtccgcgc
    15901 cggcggggag agtgttcgcg tccggcgcgg gcgtctcgcc atgcgtcgtg tcgggcgacg
    15961 cggcgttcgt tgaagcatca ggcgtttcgt tgtggttcat ggctctctgg ttgacgctgg
    16021 cctgctgcac gttcgcgccg ccttgttgag gcgcggcgca ggcgttgacg atcagcaggc
    16081 tcgccgggaa cagagcgacg aacagggcga cgggaagatg ctggcggact aagcgcatca
    16141 tatctcctta caagcctgcg ccgcgcgacc cgcttcctga gcgtgccggg cggcgggctg
    16201 acaagtgcga aactcaaggg agggagccta cggcgcgata ttatatcttg gccgacgggg
    16261 gcgtcccaac tcgtcgcgca agccgttctc ggtgagcgcg tgcgggttgg ccgtgctctt
    16321 cggcgtcttc gggtcgccgt cgaagtcgta ggggatgccg tcgttctcca acccgacggc
    16381 ctgccgctcg cggttctgca tccctatgtc gcgcgggtcg ttgtcagccg ggttcttcgc
    16441 ctgatactgc gcgtaaggct gcatcacgcc gtacacgtcg tcgtaggcgt gcgccatctc
    16501 gtgatagaga actacgaccg gatcgagatc gcgccacgcg tccgcagggt tcttcggatc
    16561 gcggaacgga aactggttga gttgcgtgtt gtaagagatg atcacgtcgg agccgtcgcc
    16621 gggctgtccg gtcttcgggt cgagcgaggt cttcgtgtag tcggtcgggg cggcgaagcc
    16681 gttgtcctct tcgctgagtt cgcggatggt gacggagttg ccgtcgttga agaagcgacc
    16741 gacgagccac caactactct tcggatcgcg gctcgaatcg taggctttat caaactccgt
    16801 aagcatctgc ctgcccgtcg gcgatgaacg cagaaactct atgtcctgct ccacgcgttc
    16861 gacaaactcc ggcgagcctt cgatgcgcac gctgcgactg ccgggctggc ctgcgagttc
    16921 gacgttgacg accgtggtgc ccgtatcgcg tccaaccgtg tcgccacgct gcgagtagat
    16981 ggtgtctctg cccgtcgcgc gttcgggatt acggttagcg gcagtgttgc cgtagagtct
    17041 gtccgtgcct tcaccggcgt agagtttatc gttgccgtgg ccgccttcga gtgtgtcgtt
    17101 atcgaggccg ccggagagca cgtcgtggcc tgcgccgccg aggacgtgat cttgcccgcg
    17161 cccgccttcg agataatctt cgcccgcgtt gccctgtatg taatcgttcc cgtcgccgcc
    17221 gtagacggtg tcgtgtcccg cgctgccgta gagtttgtcg ttgccgcgcg agccgttgat
    17281 gtagtcgcgc ccgccgccgc cgttcactgt gtcgtcgccg tccccggctt cgatgcggtc
    17341 gttgcccgct ccgccctgta tgcggtcgtt gcccgcgccg ccttcgagcg tgaaattaag
    17401 cctcacattc tgactaaccg tgagcgtatc gttgccctcg cccgcgcgga tcgtgacgag
    17461 gtaaggatcg ttgcgtccga gttgctgcgg cttgccgtcc acgttaacct gcgccttctc
    17521 gcgcccgtcc gccggatcgg tgtaggtcga agtggagatg gtgtgccgct cgccgttgac
    17581 gttgatgacg gttgcgcccg tgcgggcatc gcgcgtgacg cgtatctgat cgtcgccgcc
    17641 gcctgcgtca acgacgatgc gttcgatggg cggctcggtc gggtcggcgg cgggcgtgct
    17701 ggtgcgcttg atggtcactt ccttcgtcga gacgatgacc gtctcactcg tggccgacga
    17761 agcagccgac gaaccgccgc cacccgccgc gttgacttgc gcgttggtgc gcgctaactg
    17821 cacgcgcgcg tccaactcgg tctgctggcc gggaagggtc gaggcgggcg tctgactgtt
    17881 ggtgctcgaa gtgacggcgc ggttgctgac gagttgcatc gcattctcat cctgcggctc
    17941 ggttgagacg cccggctgcg cgatgcgtgc gacggcttca cgcgcccgct cgattcccgc
    18001 ctgcaccgcc tcgctgacct gattcgcaag ctgctggccg atctctgcaa gacccatcac
    18061 accctcctca acttaatccc ggaaattttg gttttgacgc cgacagaata gttatcggca
    18121 cggcgacgaa aatgtttccc cgccgcccac gcgtgcattg aaaagggcat gaagcgtctg
    18181 acccgtcggt gagggcttta acacgcaccg gcaggtcagt tgtcgaaacg cgccgcgcgt
    18241 ctactgtgag acgatgcggt tcggcgagtt gatgatcgtg ctgtaggaac tcgtaaactc
    18301 ggcgcggaag ttctgttcgc cgcgattgtc gccgttcggg atcgtctggt tcaggctgtt
    18361 gaagagcgcg agcaccatct cgtccggctt caggtcgcgc agcccgtttt tgaactggtt
    18421 catgaccagt tctttcgcct tctcgaccgc gccgttcttg atctcgtccc tgatcttgcc
    18481 gtcaatgggt ccgatgacgt tcttgaggaa tgtctcggcg gcggaacttc cgagtttgct
    18541 gccgatgccg gggatgaaac cgaaggcgat gtcgacgaac aatttgacgg tttgcgcctg
    18601 cgcctctgcg tccttcttca tctggtcgag cgaggacttg aggccgttgg tgagcatgcc
    18661 gaggaggccg cccgctaccg ccgacgcgtc catgcgcgac tgcccgccgt gcgtgtcgcg
    18721 ccaacgctgc tccgcgccgg gcgagtcgtc catgagcgcc gcgccgatct cggagacttt
    18781 attggagagg aagtttgcca gcgacccgct gctgtagccg agcggcggcg tgaagagtgc
    18841 ctgctggaag aacttctcca agcccggctg aaacttgagc gtgtcgaagc ccgcgccgtt
    18901 cgtcccgagg ccggacttga tgatgttgtc atactcggcc atgaagacgg cgctcatgcc
    18961 gtctttgaac atgacgttgc tacggaagtt gtcctgcgtc ttcttgtcgg tgagagactg
    19021 cgcggcggcg ttgaagaagt cggtgcggag tttgtgcagt tcatcgccgg tcacggagac
    19081 ggggcggtgg tcccagccgt cgcgcacgtc gttgtaggcg agactgtaga gcaggctctc
    19141 gatgcggcca tattcgacct gattgttcgc gccggtcgtg cccggcctgt cgtaataagc
    19201 cgccattgat tcgagcgtcg agacggtgcg ctcgcccgcc gtcgcgcgct taacgaactg
    19261 ttcgaggccg cccgcccgac ggagcaggtt gagttgcgac acctgattgt cggaagaggt
    19321 ctggccgagg acgtgcgtgg ccgccgccgc caggtctttc ccgacgccgt ccttcgtctg
    19381 cgggttggcc gcctgccgcg ccgccgcctg aaagaagatg ctcttgagat tttcgtgctt
    19441 cgcgtcgagc ttgccgaaaa gctctatggc gacctgcgga ttaaacttgc cggaggccgc
    19501 ccagcggcca accagattat ccatgtcggc ctgattgagc agaccggcct tgtcgagcgc
    19561 ggcgaaagag ttgcgcacca cgcccacgaa ttcgttgatg tcgccgcgat ccgtccaatt
    19621 ctggaaagtc tcataggaga gcgactggtt caaaagttcc gccgtgcggt cgttgccgag
    19681 cgccttgaaa tagccctgaa tccactgcgg gtcgttctgg tgttccaaca actggcgcgc
    19741 gaacatgaag ccttccgcgc ccggatagtt tgcgcccgtg cggtagaaat ctatgtaggc
    19801 ggcggcttcg gcgggtgtgg cgtctgcgcc gggtggtttc ggctcggtgc ctctcgggat
    19861 gttcgagccg ttgttgacga acgtttgcgg gacgttggga ttctgctgct gcgcgttggt
    19921 ctgcacacgc tctttgagcg cggacgcgcc gaagaaggcg tcccatattt tacccgtcgc
    19981 aaactcgacg ataggctccg gtttctcctc cggctgctgc acaatttcgg gctgccggta
    20041 cgctatctct tgaacgcccc cgccaccgtc caccttataa gtcatgtccg tcctccaacg
    20101 tgaaaatact tttacggctt cacgagagtt atcggcacac cgccggaaat gttgcttgcg
    20161 gggcgggcag agttcggcgg cgactcaggg agttgtcaag agccggacgg gcacggaggc
    20221 gagactagaa cattctctgg agttgtgaga tggcgacgcc gggcggggcg ttctccgccg
    20281 tgccctgacg caggaaagcg ttgacgctgt cgagtttgcc gagcgcgtaa tcagtgcgcg
    20341 ggtcgctgcc cttcttgtac gcgccgatca ggattaaatc cttctgcgcc tcgtaagtgg
    20401 cgagcacttc gcgcaggcgt cgcgccgctt cgacgtgcgg cgggcgggcg accgcagaca
    20461 tgacgcgcga caccgattgg ttaatgtcaa tcgcaggata gtgtccggcg gcggcgagtg
    20521 cgcgcgagag gacgatgtgg ccgtcgagta tggaacgcgt ctcgtcggca atgggttccg
    20581 tcatgtcgtc gccttcgacg agcaccgtgt agaaggccgt gatcgagccg tgcgcgctgt
    20641 tgcccgtgcg ctccaacaat ttcggcaact cgctgaagac cgaaggcgga aagcccgcgc
    20701 gtgcgggcgg ctctcccgcc gcgagtccca cctcgcgcaa cgcgcgcgca aagcgcgtca
    20761 ccgaatccat catcaacaac acacgcttgc cctgatcgcg gaagtgctcc gcgaccgccg
    20821 ttgccacgta agccgctttc atgcgcacca gcgacggctc gttcgacgtg gccgcgatca
    20881 cgacggaacg gcgcattccc tcttcgccca agtcctgttc gataaagtcg cgcacctcgc
    20941 gcccgcgttc gccgacgagc gcgatcacgt tcacgtcggc ctccgtgttg cgcgccagca
    21001 tgccgagcag cgttgacttg ccgacgcccg ccgccgcgta gataccgacg cgctgcccct
    21061 cgccgatagt cagaacgccg tcgagcgaac gcacgcccgt cgccagcatc ttcgtcacgc
    21121 gctggcgacg caaagcatcg ggtggccgcg cagtcacggg tcgctcctgc ccgccctcaa
    21181 tcgcgccttt gccgtccgcc ggttcgccca gcccgtcgag cacgcgaccg agcaggccat
    21241 cgccgacgcg caccttcaaa gacgcgcccg tcgagacgac ttccgcgccc atcgcgacat
    21301 cctgaatatc gccgagcggc atcaggatca cctcgctgcc gcgaaagccc acgacctccg
    21361 cgcgcaactc ccgcgccctg tgcggcgagc gaatcaggca caactcgccg acgcgcgccc
    21421 ccggcacgct cgcgcgaatc agcaggccga ctagttccgt gacgcgcccg cgcacctcca
    21481 ccggcgaaag ccagtcaacc gcctcgatgt actccgcaag gtttacagac ataacttatg
    21541 gacgctcctc acgcgcccgc agcctaacgc gcactctaaa cttctcgtcc gttcagagtg
    21601 aggcgtgaaa gctaaaccgc tcacccgttc gagatgaaat tccgagtctc aaccaaaccg
    21661 cgagatcagc ttctcacgat ctggaattct catggcctgt tgtcgccgtc ggcgcgtgcg
    21721 agcagtgcgc gttcaagcac gcccagttgt acgtcgagtt gcgcgtctat cgtgcccgat
    21781 tcgctttcga tgacgcagcc gccgtgcgtg acgcgcgggt cggccacgag atcgagaaac
    21841 cgtgcgcgcc ccgtggggtc gagtcgggcg cggtgcgcct gcacggcggg caggtctgcc
    21901 gggttgacgc gcacggtgag catctcgtgc tgtcgcgcgt tgcgcaaggc ggcggcgacg
    21961 atctcggcca gcgtcgcgtc gtcctgcgcg atctcgcgcc cgatgatctt ttccgccagt
    22021 ttgacggcga gacgcaggag gtcgcgttcg gcctcggcga gtgcgttgtc gcgccgatca
    22081 cgcgcgtcga gcaggtgttc gttgagttcg gcgagcgcgg cttcgagtcc ttcgcggtag
    22141 gccgcatcgc gcaactctaa agccgccgct tccgcgctcg cgcgcaaggc tgccgcctcg
    22201 cgctcggcgg ctgcgatgat gagtcgcgcc tcggcctgtg cttccaccac cggacgcttg
    22261 acgaccccgc gcgctttgaa gtctctctgc gcgcccgttg cagcaccctt gataaccttg
    22321 cccgcgctca tgttctaacc tcgaaggctt cttcctgcat gttgacgact gcctccttcg
    22381 tcgcgccgcc cgccgtctta acgtgacgca ggctcagcgc gagcgactcc gcttcacggc
    22441 ctatgacgcg caagacctca tgctcataac ggacgctgca atctgcgacc atgcgccgca
    22501 gagcgcgcgc cgcttcgaag gggagtttct gcgccacgta ggccgcgcgc tccgcagacg
    22561 actcgcgcgc cgccagcgag gccgccaaca catgcatccc caaccaatcc agcatcgctt
    22621 ccggctcctg ctgttgctcc aacgcttcat gcaccaaccc ttcggccagc gtgacgcgcg
    22681 ccgattcgag tttgaggagc gtcgccatgt gcgcgaggat ggcgcgcgcg ttctccgccg
    22741 agaagcggcg caggaacgac cccaccgcct ccgcgcgacc aatcccccga cacgccaccg
    22801 ccgtctcgtg cacgccgagc agccgcacga gccgcgccaa ctcgacaccc gtgagcgttt
    22861 cgagcggcga agggtttctc agttcgccgg gggcgacgaa gttcgacaga aaaactttgc
    22921 gcactaatcc gacgagttgc gccggaggct ctgcgccgtc ccacgcctcc gcctgaagcg
    22981 cgccggtgca ggcgtctgcg aacgaaggcg gcaggtaacg caaaatgagc gcgcggacgc
    23041 gcggcggctc gtcgcgcaag gcttcgagga tgtgcgaagg gtgaacgtgt tcgtcgagtt
    23101 gcgtgcgact gtggttggcg cgcgccagcg tccgcgtcag ccagtgcgcc tgcttatcgt
    23161 gcgcgagttt ggcgtaccac tgcgcgcgcc gttcgagcga ctggcgcgcg gcttcattca
    23221 gcgcgtcgaa gaggccgtat gcagtcgtcg cgttcgcatc ccccgcgtcg tgcgcgtgct
    23281 cgtcgctttc aaacgcactc tgcccgaaag cgatgctcag ggcgagcagc gtgtcaatgc
    23341 gttcgggttc cacaggttga gccattactt aagtccgtcc ggcgcgaagt ctctgtgcgc
    23401 cgcagcaaat cctgaacgtg tcgcaagccg cttccgcgct tcacttcact gaggtggggt
    23461 cttccttgat cgcgcccgcg cggtttgccg tcaaggtcgc gtccggcaac tgccgcgcgg
    23521 ttgtagtcgt ctcgcgcaga gaaggcgacg ccccggcggg gagcatcgcg ggctgttcgt
    23581 ccgacgcctc ctgtactgcg atggtcgctt cctcgccttc tcgtagcacg gcgatctcgg
    23641 cgcgttgtcg tcgcgtctgg agcagaagta tgacgagcag gaagcagaag atcgtcaata
    23701 gccctgcacc gattgtcagc agcagactat tgcgacggcg cacgttctct tggggtcgga
    23761 gcggcggcgg cgtctcgaac aacatcgaga cggtgacttt ctccggcttc aaacccggca
    23821 cgccgcccgc cacctgattc tgcacctgct cgcccgtgaa ggtgggctgc ggctctttat
    23881 gcacgagcag gacgctggcc gtcgccgggt aaggatcgag tttgatcgtg tcgtcttcgg
    23941 ggagaacgat ggttacgttg gcgcgcgcca cgccgggcag caggcgcagt ttgcgctcca
    24001 tttcgatctc gcgcacgcgc aggcgttgcg cgttctcgac cgccggtgag gggaagacgc
    24061 cgccgtcgtc cttcgccagt ttcagttcat cggggcgcgg cagtccgtag tagcgcaaca
    24121 cctgcgtggc gcgcgcgctt tcgtcgccgc cgaaccagcc ctcgtcaatg ttgatgctcc
    24181 actgcgccgc ctcgccctcg ccgacctctt ccttattcgc cccgatctgg tactccctga
    24241 gacggtcaat gatctcgatg gcctcgctct ccgaagcgac tctcgccacg gatttttcgc
    24301 cgcacgcgct cgtcaaaacg gcgacgaggg caagcagtat tccgaatatg acgcgttggc
    24361 taagggcgcg aaaatcggcc agcctatgaa tttgtcgcat gttggttctc cgcgcaagac
    24421 ggcgtcgaaa tcgtccacga cgattgactt gagggaatat gatgtccgca tcttaccgca
    24481 aacacagggg caaaagccat cgggttttcc ccgcgcctta cgccacgaac gcgccagcgg
    24541 tcttgccgcg cgtcgcaaca atttaattct tccgtcgccc caaatcggct ataatccgcg
    24601 tcgccgaaac gcatccccgc ttaactctca agcacgcttc ccccgcgtct ttcacgttat
    24661 gcacgacttt gcggccacaa catcactcgc cgaccgcgac caactcatcg agcagcatcg
    24721 ctcgtatgtc cgcgcgctcg ccatcaagac catgcaggcg atggcggcgc gcgtcgatct
    24781 gcaagagctg atcgcttacg gcgacgtggg cttgatcgaa gcagccgagc gtttcgaccc
    24841 acggcgcggc gtcgccttcg ccaccttcgc gcactatcgc atcaaaggcg cgatctacga
    24901 cggcctgcgc gagatgggcg tcttcacgcg ccaacactcc tcccgctcac acttcgccgc
    24961 caacgccaac gacctgctgc aatccggcgc ggacgacgag caggccgccc tcgccgaagg
    25021 cggcgcacgc gccatcgaga gcgagatcgc aaccacgcag tctctcatcg acgcgctcat
    25081 ccccgcctat ctcctctcgc tcgacagcga cgcgatgccg gagatcatgg atcaacaggc
    25141 gctctcgatg gaacagatcg agcagcgcga gttgatcggc tttgtcctac atctcgtcag
    25201 agaactgccc gaagacgagc aacagttgct cgacgcgctc tacttcaagc atcgctcgat
    25261 gacggagctt gccgccgaaa tgggcatctc caagtcttgg atttcacgtc tccactcgcg
    25321 cgccatcaag cacctgcgcg accgcatgca ggagcgcggc atgctcgaag ccgacagccc
    25381 ctcataaaac tctctccatc acccgcacac gcataaaacg cgtttgttcg cccctcactc
    25441 aatttgacac gaaacacttt tcgccgcgcg ccgataattg agccagaaag atcataccgg
    25501 cgggcgagag gaagtgtccg cgtcgcaatt attgcccgcg tagtttcaga agaggagcga
    25561 aaacaatgtc gaacgtatct ctactccaga gccagtcaat caacttcaac tttaatttca
    25621 acgggttgtt cggcgcaggc gcgaactccg agttgctcgc gctgctcgaa gcgacgagcg
    25681 atctggcaga cgcgctcggc aacttcaacg acaaattcgc cgcgctcgca gaaaagttcg
    25741 gctccacttc cgcgctctgc cagtgcgcgc cggtcgcgcc gcccgtgctc gaagattcgt
    25801 gccacccggc gggcagcctg cgcgcagagg gcgacacgat cacaacgccg ggcggctaca
    25861 agatcgagat gctcagccag tacgagtgga agatcacggg gccggacggc aaagagacgc
    25921 gcgtgtgggg cgacccgcac gtggacgaag gcgacggcgg caagtgggat ttcaaacgcg
    25981 actccacctt cgtgctcggc gacggcacgc gcatcaacgt caacacagtc ccctacggcg
    26041 acatgaccgt gaccggctcg ctcgaaatca tcagcggcaa cgaacgcgtc ctcgtctccg
    26101 acatcgacca gggcaaaggc aagatcggca ccgtgacaca ggacggctac cagcacgcca
    26161 acagcttcgg cgacaacgac gtgtttgtga tggggcgcga aaccgacgac tggtcgttca
    26221 ggggcagaga gatcacgggc agcgagaacg gcggcgagtc gttcaagacg ggcggcgagt
    26281 tgcacccgct caccacacag gtcaatcgcc acggcaatcc gcgcgactgg gctaactcga
    26341 tcttcgacag cctgctcgac tcttggcgcg gcgacttgcg cacgaacgat ctcggctaca
    26401 acgcctactg cgatagagac aactccgggc gcgtcgaaaa cgacgaacgc gatgaccggc
    26461 gccaacatgc agaggctttg cgcgacgcct tccaagccct cgcagacatg ttcgacgccc
    26521 tctcgcgctg gctcacgctt
//
LOCUS       DASWNS010000464         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_17781, whole genome shotgun sequence.
ACCESSION   DASWNS010000464 DASWNS010000000
VERSION     DASWNS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17781"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2470)
                     /locus_tag="VGB76_15995"
     CDS             complement(<1..2470)
                     /locus_tag="VGB76_15995"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEY0100447.1"
                     /translation="MSNPLVEIMKLGQSIWYDNIRRAMLVSGDLRKKIEEDDLRGVTS
                     NPTIFEKAITGSNDYDDQLRALVQAGKSVPEIYEDLVVEDIGKAADILKPVYDKTDGV
                     DGYISLEVNPGLAYKTQETIEEADRLFQRLGRKNVMIKIPAAQEGLPAIEESIYRGVN
                     VNVTMIFSIENYEQVAEAFIRGLERRAAEGKTVDHIASVASFFVSRVDTAIDTDLEYK
                     ARHASTPEEKARLEALLGRAAIANAKLAYARYKEIFHGERFSALKAKGAQVQRQLWAS
                     TGTKNPKYSEVLYVDNLIGPETVNTVPPATYTAIREKGKVALTLEEGLDECRTLVREL
                     GEIGIDLKAVTEKLQKDGLSSFVNSFDTLAESIESKRDAILSGINERLTASLGKYSEA
                     VAGAIKEADKGDVMRRIWRKDAALWKSDEAHQKIIKNALGWLTVPDMMIGVEDDLVQF
                     ADRIRNVREFKHAMVCGMGGSSLCPEVLRQTFGKQEGYPELLVLDSTDPDAFSGIADK
                     IDITHCLFIISSKSGTTTEPLVFYKYWYDQVSQRKENPGECFVAVTDPGTLMEQMATD
                     DKFKRIFLNPADIGGRYSALSYFGMVPAALLGLDVRKLLDRAERVVHACASVVPAAEN
                     PGARLGAILGECAKAGRDKLTISTDPKISSFGLWVEQLIAESTGKEGKGIVPVAGETL
                     GAPSVYADDRLFVHVSVEKIDSETETKLKALEAAGHPVVYRTLTDLYDLGEEFFLWEI
                     ATAFAGWRLEINPFDQPNVQESKDATKELLEAFTRDGKLPEQDVLATDGVLTIYADER
                     TRAELPGTSVSEAIKAHLARASTGD"
BASE COUNT          442 a          778 c          772 g          580 t
ORIGIN      
        1 agtcgcccgt ggacgcgcgt gcgagatgcg ccttgatcgc ttcgctgacc gacgtgccgg
       61 gtagttcggc gcgcgtccgt tcgtctgcgt agatggtcag cacgccgtcg gtggcgagca
      121 cgtcctgttc gggaagcttc ccgtcgcgcg tgaaggcttc gagcagttcc ttcgtcgcgt
      181 ctttcgattc ctgcacgttc ggctgatcga aaggattgat ctccagtcgc caaccggcaa
      241 aggccgtcgc gatctcccac aggaaaaact cttcgccgag atcgtagagg tctgtcagtg
      301 tgcgatagac gacgggatga ccggcggctt ccagagcctt caacttcgtc tccgtctcgc
      361 tgtcaatctt ctcgaccgag acgtgtacga acaggcggtc gtccgcgtag acggaaggcg
      421 cgccgagagt ttcacccgca acgggcacga tgccctttcc ttctttgccc gtgctctccg
      481 cgatcagttg ttcgacccag aggccgaagg aggagatttt cggatcggtg ctgatcgtca
      541 acttgtcgcg cccggccttc gcacactcgc cgaggattgc gccgagacgc gcgcccggat
      601 tctccgccgc aggcacgacc gacgcacacg catgcacgac gcgttcggcg cggtcgagca
      661 atttccgcac gtcgagtccc aacagggcgg cgggcaccat gccgaaataa gagagcgcgg
      721 aatatcgccc gccgatgtcc gccgggttga gaaagatgcg cttgaacttg tcgtcggtcg
      781 ccatctgctc catgagcgtg cccgggtctg tgacggcgac gaagcactcg ccgggattct
      841 ctttgcgctg gctgacctga tcgtaccagt acttgtagaa gacgagcggc tcggtcgtcg
      901 tgccggactt cgaggagatg atgaagagac agtgtgtgat gtcgattttg tctgcgatgc
      961 cggagaaggc gtcggggtct gtggaatcga gcacgagcag ttcggggtag ccctcctgct
     1021 tgccgaaggt ctggcgcagg acttccgggc agagcgacga accgcccatg ccgcagacca
     1081 tcgcgtgctt gaactcgcgc acgttcctga tgcggtcggc gaactgcacg aggtcgtctt
     1141 cgacgccgat catcatgtcc ggcacggtca accagccgag cgcgttcttg atgatcttct
     1201 ggtgcgcttc gtccgacttc cagagcgcgg cgtccttgcg ccagatgcgg cgcatcacgt
     1261 cgcccttgtc cgcttccttg atcgcccccg cgaccgcctc actatacttt cccagagaag
     1321 cagtaagcct ttcattgatg cccgacaaaa tggcatcgcg cttggactcg atggactcgg
     1381 ccaacgtgtc gaacgagttg acgaaggaac tcagcccgtc cttttgcagt ttctcggtga
     1441 cggctttcag atcgatgccg atctcgccaa gttcacgaac caacgtgcgg cactcatcca
     1501 gaccctcttc gagcgtgagc gcgaccttgc ccttctcgcg gatggccgtg taggtggcgg
     1561 gcggcacggt gttcaccgtc tcaggaccga tcaggttgtc gacgtataag acttccgaat
     1621 acttcggatt cttcgtgccc gtcgaagccc agagttgtcg ctgcacctgc gcgcctttgg
     1681 ccttgagcgc gctgaagcgt tcgccgtgaa atatctcttt atacctggcg taagcgagtt
     1741 tcgcgtttgc tatcgccgcg cgtccgagca acgcttcgag gcgcgctttc tcttcgggcg
     1801 tcgaggcgtg gcgcgctttg tattcgaggt ctgtgtcaat cgccgtgtcc acgcgcgaga
     1861 cgaagaagct cgccaccgac gcgatatgat ctaccgtctt gccttcggcg gcgcggcgtt
     1921 cgagtccgcg tatgaaagcc tccgccacct gctcgtagtt ttcaatcgag aagatcatcg
     1981 tgacgttgac gttgacgccg cgatagatgg actcttcgat ggcgggcaag ccctcctgcg
     2041 ccgccggaat cttgatcatc acgttcttgc gcccgagcct ctgaaagagg cggtcggcct
     2101 cttcgatagt ctcctgcgtc ttgtaggcga ggccgggatt gacttcgaga ctgatgtaac
     2161 cgtcaacgcc gtcggtcttg tcatagacgg gtttcaggat gtcggctgcc ttgccgatgt
     2221 cttcgacgac gaggtcttcg tagatttcgg gcacgctctt gcccgcctgc accagcgcgc
     2281 gcagttggtc gtcgtaatcg ttcgagccgg tgatcgcttt ctcgaagatg gtcgggttgg
     2341 aagtaacgcc gcgcaggtcg tcctcttcga tcttcttccg gaggtcgccg ctcacgagca
     2401 tcgcccgccg gatgttgtcg taccagatgg actggccgag cttcataatt tcaactaagg
     2461 gattactcat ctttcgctcc ttcgtggctt aagaacagag gtcagagggc ggaggttagt
     2521 cgagagcagt tttcgccagc ctctgcccga ctatttatac tccagccctt ta
//
LOCUS       DASWNS010000465        11991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_327455, whole genome shotgun sequence.
ACCESSION   DASWNS010000465 DASWNS010000000
VERSION     DASWNS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11991
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327455"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VGB76_16000"
     CDS             <1..301
                     /locus_tag="VGB76_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100448.1"
                     /translation="SYYEYEQGGTPFEDFAARLDRNQKVECRAIIDLLMKWGDSLEGE
                     QCLTHACSQGELKEFQGDLVRVFYLCQSEALAIIIIDCLLRDENGVLFEAIRRKV"
     gene            307..672
                     /locus_tag="VGB76_16005"
     CDS             307..672
                     /locus_tag="VGB76_16005"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0100449.1"
                     /translation="MPKAKPKPLKVTHKPLADLLNDGSKEFEELTKKRLAQLDIAQDI
                     IKRRKALGLSQTELARRAGVNQPLISRIESGNFKNFEIQTLIRIATALEARYDFNLVV
                     DPQEPDAFSPPAATRGASA"
     gene            1171..3093
                     /locus_tag="VGB76_16010"
     CDS             1171..3093
                     /locus_tag="VGB76_16010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0100450.1"
                     /translation="MTSKKGGAEMTLKAANDLTTFAWNVLEKLYTLFTKLWPPVQLFL
                     ALVGLALAMFFGKELLHSLLSSQSGISRALEAKLEPSRNADQGITAFLLSNVKDEWTN
                     PDLLNVKELDSILREVDTKVQDVSKANIVTEGELSDRSPLVSEMIDKLGLKVVNSGTT
                     APWQSAITSMKSERFMMVPATSLRRKTLNEPPLKPDDKPQLEVTLTHNPEILFDLHLA
                     SEIEPIMRKMDGASGKLLTIVQTYFITESGVFLIRASGVKDHGRYYGTEFQTYTQYMD
                     RPYFWEAVAGKQRKVTPFDYGSKPYIDLGGNGFVVTFSKKFNLPNQRAGVLCVDAKLP
                     DYVTDDIEQYMKSLGAKVSDFYWSEDRSIEPGKDGPLPSSFSWFAVQLNKSREARSQV
                     LGNIATEPVKATPSNPQGQANSTVRFTVPVTSTEAGPGMKRTKLLWVEFDSNSILTTL
                     TWNLVFFTVGIVIVIAVTGSLFRNYTVLKREMSNVLEKMSKVMRDASTPFVWLDEKNG
                     FVDVNNRMLKLLGYDNIEELRMHSATFRGLITAQTQQTYDDILTTSGAGRETGEYEIN
                     VVTKSGEVLHVRAHGERIPYPTFWRSGLPHRFGIFVEVTELTSATRPNQVTEKRDLES
                     VPNPTLKEVPTDERAA"
     gene            4207..7311
                     /locus_tag="VGB76_16015"
     CDS             4207..7311
                     /locus_tag="VGB76_16015"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0100451.1"
                     /translation="MGFHVRVQAAGHFIPLPQPFATGVYGFRTTAHSLFKFDESAATP
                     NTGVAIRASFVSQGSTVRPGDERDIRSTVEAFFSFFSKKDLNGVLALWSEKSADLNQF
                     REQTKQRFAAIDTTFANLAFSRVTAEGERARVRVAADRTANDTESRRSRTLHVLYDLT
                     LVKEGGRWLILGVEDAIEKLVAAVIAAQTEDERDALLASDPELVSITFVDTLNRSGQR
                     LFYYGNVQGAITAFTTGHRVGVELHRKNPTPTTRRLVAVMLSSLGVMNSVRGDYAKAL
                     EYLEASLAEAKDDDLVASLKQNMGILRALRGNYEAALELFRESLERAKALNDRRQMGQ
                     ARDGIANVFSLQNKFDDALREYEKNKEMREEALREKTNSNALPADIQKYASDLADTLN
                     NIGIVYHQRWHAMRGRNRDKADLLEANKYYAASMKHVEGRDAIREATTRLNQAELQVL
                     LGDYKAALVSASGAADLGERLGFAEVVWRSWLAKGYALQGQQQQEQARQAFGRAIELV
                     EDLRRQSPGDEVERQKFFEDKVAPFHAMVGLLAAEKNKGEALFYSERAKGRVLLDVLQ
                     GGRLLVNKAMTAEEQRQEQEFQRQLDLQDRAVSEAWGGRREGVEFKLLLDRRSKLERD
                     YESFLQRLYEAHPELKVRRGEAQAVRADEVVRLLPDASAVIEFTVTADKTYVFVLTRS
                     RGAASTQRAGDAANLTLYTVDITKEELRDRVARFREMIGRVDPGYKKPAAELFQLLLG
                     RASPQLKDVKTLVVVPDSYLWELPFQALQRPGGHHLLQDFVLLYAPSLTVLREMMNQR
                     PGGDEAMRPTLLALGNPSLDGDVLQRVEAARMDAELEPLPDAEKQVNELRDVVYGHSL
                     SKVYTGPDAREDTFKEEAGRYRILHLATHGILNNAQPMSSYLVLAPPRAPRAQGDDGL
                     LEASELARLNLRADLVVLAACETARGQVGEGEGMVGIAWSLFVAGCPTTVASQWKVRS
                     DSTRLLMLEFHRNVVGDLGTRPSHIRAARALREAALRVFNEKELDYKHPFHWAGFIAI
                     GDGL"
     gene            complement(7343..9703)
                     /locus_tag="VGB76_16020"
     CDS             complement(7343..9703)
                     /locus_tag="VGB76_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100452.1"
                     /translation="MFGRVPESAQLERVFQLCYAVLGDEDAAFAATVNCALALDVEVK
                     EQRARKRYRIDASGFRQKLIMRRELALQFIAFRLCHKEELAQEARWREGLGPMPHESY
                     FVTRYIKHILYMTVPHRSFQVAVALGRLLYDYLKREVYLVHDSLAQDPRLRKNDQMYK
                     DWKLALMNELHEARFKGLLTVKGGKQGAKSFETHGQQEQLVGLVENVLKLLSPWGTAC
                     ALEAGSRSASPFRNFRFTGHPDKESPFEEKRMRAIIDPDCLTAVTRELKVPPPVERLA
                     VPLFNLPDQPDQIPPVDFNKLPQFTVDRLRKLFDEVTGGRRRRRKARRTELSVEVDGV
                     ERTSFDPAEQSEVTFTLERGDDLIQVVASDEDGPLLLGSLLLSSSGALDGEEDWKGVI
                     RLEGGQRVNVTVTPLPASAGAARGANVVVAYSEGDLFGWLRGAAWAVGDRIKSWTAGA
                     QPGAWGLALRYVAVFGVAAVLSVAAWVFYTGKVELAFWRPKTVEQPTAVETPGERKEI
                     VAQVLPSPEDKGHNRPGQSGVVGTNRSPSPTPLPGKDAAPVLPADLGLVVMDGVNTFV
                     LDEQGNVSGINKTLPKSYRAALELTLSKQQISVAPLPSELTEVAGQVMGSPSADVEFT
                     LLSPAGEVVPTTTPVLTWNPLDKAKGYKVEVFDEQFNPVAESPELHTIKWQVPKGKLR
                     HGHAYSWKVTAFKEGGEVSSHRPDDAGGRARRLSQTKFKVMESSAAEMLRSAKGKYNS
                     RLLAAIVYARSSMPEQAEGELQALLRDNPGSAVLQKLLGELRSQRR"
     gene            10085..10942
                     /locus_tag="VGB76_16025"
     CDS             10085..10942
                     /locus_tag="VGB76_16025"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEY0100453.1"
                     /translation="MSVLNNRPSINSVKAKAISFTCTVALLLFFSVVNTVNVHATVPV
                     PSGFTAISSGTGVTVYRKNYTGGQPDFVTVVDLRYATLQSYTGWAQSDGTVERRSLTT
                     HWQNAVAQNTSTRIAKVAINGTFFDPNMSPYTGIAFGLKANWWIMSRGYAAGTEYPGL
                     IRTLTYDATYNSSSIQAYNSTTFDQGISDVVGGLDPTANKDRYSNIPRTFVGVRDDNG
                     DGHCETVIFFSSKYATQAWATNILTGFGTVARMMLDGGGSTGLVVDGTQHIPTSRTLP
                     HVFIVSAGK"
     gene            11050..11910
                     /locus_tag="VGB76_16030"
     CDS             11050..11910
                     /locus_tag="VGB76_16030"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY0100454.1"
                     /translation="MRFSYRKRHLAGVLSLYLALLGGILAPQSGWTQAFAASPVAPQV
                     YGPYYVQSPVYGGWSGDEKWYNVSGSSMHKAFATFDDSNAYDINLPSNRENMLPVYSS
                     GYGKVTSLGTTFPGTTDGGTYGAVLIDHQNSTYTGYMHMRNIQVRAGNSVTPNTIIGY
                     VSDKGVPGVVHLHFAFYLKIKKNGKDYLSSASTYIAPRDFGLSLPRSISLRRGASFQV
                     SASVPFPSITNSRVQDQLWYNNTYWTSSNSRVATVSGSGLVKALAAGSSTITLKFSGK
                     TFNIPVNVTN"
BASE COUNT         2635 a         3528 c         3419 g         2409 t
ORIGIN      
        1 ttcatattac gagtacgaac aggggggcac tccgttcgag gacttcgccg cacgtcttga
       61 ccggaatcaa aaggttgaat gccgtgcgat catcgatcta ctaatgaaat ggggagactc
      121 tcttgagggc gaacaatgcc tcacgcacgc ctgcagccag ggagagctta aagaatttca
      181 aggcgactta gtgcgcgttt tttacctctg ccagtctgaa gccctcgcca tcatcataat
      241 cgactgcctc cttcgtgatg agaatggggt gctcttcgaa gctatacgca gaaaggttta
      301 gtaggtatgc caaaagcaaa gcctaagccc ttgaaagtca cgcacaagcc gctcgcagat
      361 cttctcaacg acggcagtaa ggagttcgag gagttaacca aaaagcgact ggctcagtta
      421 gacatcgcgc aggacatcat caaacgcagg aaggctctcg gcttatcaca gaccgaactc
      481 gcgcggcggg cgggcgtcaa tcagccgctt atttcgcgga tcgaatccgg caattttaaa
      541 aacttcgaga ttcaaaccct catccgtatt gctactgccc ttgaagcacg ctacgacttc
      601 aatcttgttg tagaccctca ggagcccgac gcgttcagcc cacctgctgc cacccgcggg
      661 gcttccgctt aagccgcatc ccgcggctga accagctcaa cagaactctc cctccaagcc
      721 acctgcggac aactcagccg aggctcaccc ctaccctgaa tctcccgcct atttactcga
      781 tgaataggcc cgtcacccgg aaaaggtagg ttccacctct ccgcccagac cacaacctct
      841 tcccaacatc caaaaaaatg ggttgatctt atattcttta agatataaac tgcctatatg
      901 tttaaagcta taaagtcaac tgaaggctca cccatagtga tatatagtta atttctaaaa
      961 catttaagta gcgggaactg atctcaacag gtttgaagag tttatcggtg ggtagagaac
     1021 aaaacttact ctgtcgattg catgacttga acccctctca aacgcatttg tgagggggat
     1081 gcgacaggga tgtcggtcac cgtcgcccag cgagtgcagt cgcggcgccc catttacctt
     1141 cacacgcgaa atggtaaagg gtgaacaggt atgacttcca agaaaggtgg tgcggagatg
     1201 actttgaagg ctgccaatga tttgacgact ttcgcatgga atgtgctcga gaaactttac
     1261 actctattca ccaagctatg gcctcctgtc cagctcttct tggcgctggt cggcttagcc
     1321 ctcgccatgt tcttcggaaa ggaattactg cacagcctac ttagcagtca gagtggtatt
     1381 tcgagggcgc ttgaggcgaa gctagagcca tcgaggaacg cagaccaagg catcacggct
     1441 ttcctgctct caaacgtgaa agatgagtgg actaaccctg acctcctcaa cgtcaaagaa
     1501 cttgattcaa tcctgcgaga ggtcgatacg aaagtgcagg atgtgtctaa ggcgaatatc
     1561 gttacggaag gggagctaag tgacaggtcg cccctcgtgt ccgagatgat agataagctt
     1621 ggcctaaagg tcgtaaattc cgggacgact gcaccatggc aatcggcgat taccagcatg
     1681 aaaagtgaac gtttcatgat ggttcccgcc acatcactcc ggcggaaaac gctgaatgaa
     1741 ccgcccctta agccagacga caagccccaa ctggaggtga ccttgacgca taaccctgag
     1801 attctgttcg atcttcacct cgcgtctgaa attgagccga tcatgagaaa gatggatgga
     1861 gccagcggta agttgctcac gatcgtgcag acgtatttca tcaccgagtc tggggtgttt
     1921 cttatacggg cgtctggggt caaagaccac gggaggtatt acggaaccga gtttcagacc
     1981 tacacccagt acatggatcg cccatatttc tgggaggccg tcgcgggcaa gcagagaaaa
     2041 gtcacgcctt tcgactacgg gtcgaagcct tatatcgact tgggcggcaa cggcttcgtg
     2101 gtcactttct ctaagaagtt taacctgccg aaccagcgtg ccggcgtcct ttgcgtcgat
     2161 gcgaaactgc ccgactacgt cactgacgac atagagcaat acatgaaaag tctgggggca
     2221 aaagtctctg acttctactg gagtgaagac cggagcatag agccaggcaa agacggccct
     2281 ctgccttcga gcttttcgtg gtttgctgtc caactcaaca agagccggga agcccgttcg
     2341 caggtgttag ggaacatcgc gaccgagccg gttaaagcga cgccgtcgaa tcctcaaggt
     2401 caagccaaca gtactgtgag gtttaccgta ccggtcacca gcaccgaggc cggcccgggg
     2461 atgaagcgta ccaaattgct ctgggttgag ttcgactcaa acagcattct aacaacgttg
     2521 acctggaacc tggtgttttt caccgtcggg atcgtcatcg tgatagctgt gaccgggagc
     2581 ctcttccgca attacaccgt gctgaaacga gagatgagca acgtccttga gaaaatgtcg
     2641 aaggtgatgc gggacgcttc caccccattc gtctggttag acgaaaagaa cgggttcgtt
     2701 gatgtaaaca accgcatgtt gaagttgctc ggctacgata acattgaaga actgaggatg
     2761 cattcggcca catttagagg cttgataaca gcccagaccc agcagacgta tgatgatatt
     2821 ttaacgacta gcggggctgg ccgcgagaca ggtgagtacg agatcaatgt cgtcacaaaa
     2881 tcgggcgaag tgttacacgt ccgagcacac ggcgagcgca tcccttaccc gacattttgg
     2941 aggagcggcc ttccccacag gttcgggata ttcgttgaag tgaccgagtt gacgtctgcc
     3001 accagaccga accaagtgac agagaaacgt gacctagagt ctgttcccaa ccccacactg
     3061 aaggaagttc caacagatga gcgtgcagcc taaatttcgg aacgcttgat gtatgtcgtt
     3121 gcgcctctcg tatctcatct tgacgtggtg ttggcggtta aggcacacgt cagaaacaaa
     3181 tttgccaacg atagcctcct cagtcgggaa gtgtccacgt ccggctctct gtgaagcagc
     3241 ggttggtgtc aagagttttt ttatctcacg catcgacttg ccacgagcac accaataccg
     3301 ttcgtacgcg agttcctttc acctctctgt tcgcgctctc attagagcga ttacttggac
     3361 gcccagcgca agtttggatt tgggggcggg aggcgctact cctttgacgg tgctctcagg
     3421 gtcaagccga ttgccgcgct cgcccgtgct ctaaactcac tgaagccaat gtctcaatct
     3481 caacgcctaa gccgcccatt acagagccgc gggcaacata ggcgcgcctc ccggccatag
     3541 caactgcggg tcgaactcta tcccggcgcg ccagactgcg aacatgatgc cagccagttt
     3601 ccgcgccagg gcgactacag ccgtcgccct ccctcggtgt tgcgcgatcc gtgccgtcca
     3661 ctcatgcagc gccttaattc tctcagtctt ccatcgtagt aatgccatgc cgcctcgacg
     3721 agtagtgacc cagctcgcac gctccccgcc ttgccgaccc gtccgcgtcg ctgcttctcg
     3781 cccgaatcgt acccgcgcga taccagcccc aagtaggagc ggacgtgctt gcctccgggg
     3841 aagcgtgacg aatcatcggc gtggcggtaa aagagggcga ccgcgtaagt gccgacccca
     3901 aaatagatgc ttatcaagct gtccggagag cagaggtgag tggcgtaaat tgacgtatcg
     3961 taacctgaac tagaacttat tctgcttcaa gagtatgatt tccagaaagc gttggacgaa
     4021 ctggagtgtg gattacagct cacgtgtgat tattgcagca cagtcgtgaa actgtgtccc
     4081 gtccgaagca ttctcaaaca acccgtccaa aactctacga aaggggttca cctcactatg
     4141 ccgccgcgag gccatgctcc ctcatctctg aaaacactct cgattcttct gacgtttgta
     4201 ttggctatgg gctttcacgt gagggtgcag gccgcggggc atttcatccc actgccgcag
     4261 ccgtttgcta caggcgttta cggcttcagg acgaccgctc acagcctatt taagttcgat
     4321 gagagcgcgg cgaccccgaa taccggagtc gcgattcgcg catctttcgt atcgcaggga
     4381 tcaaccgtcc gtcccgggga tgagcgcgac atccgctcga cggtcgaggc gttcttctct
     4441 tttttttcta agaaggactt gaatggcgtt ctcgcgctct ggagcgagaa gtcggccgac
     4501 cttaatcagt tcagggagca gactaagcaa aggttcgctg ccatcgacac gacctttgcc
     4561 aacttggctt tctcgcgcgt tacggccgag ggggagagag cgcgcgtacg cgtggcagcc
     4621 gaccgcactg ctaacgacac ggagagtaga agatcacgca cgctacacgt gctgtacgac
     4681 ctgacgctcg tgaaggaagg cgggcggtgg ctcatcctag gcgtggaaga cgcgatcgaa
     4741 aaactcgtcg cggctgtcat cgccgcgcaa acggaagacg agcgcgacgc gttgttagcg
     4801 agcgaccctg agcttgtttc catcaccttc gtggacacgc ttaaccgttc ggggcaaagg
     4861 ctcttttatt atggaaacgt gcagggcgcg atcacggcct tcacgacggg gcatcgcgtc
     4921 ggtgtcgagt tgcaccgcaa gaatccgacc ccgaccacgc ggaggctcgt ggccgtgatg
     4981 ctgagtagtt tgggcgtcat gaacagcgtg cggggcgact acgccaaggc gctggagtat
     5041 ttagaggcga gcttagcaga agcgaaggac gacgacttag tcgcgagcct caagcagaac
     5101 atgggcatcc tccgtgccct gcgtggtaat tacgaggctg ccctcgaact cttccgcgag
     5161 agccttgagc gtgctaaggc tttaaacgac aggcggcaga tggggcaggc acgggatggg
     5221 atcgcaaacg tcttctcgct tcagaacaag ttcgacgacg cgctgcgcga gtacgaaaag
     5281 aacaaagaga tgcgcgagga ggcgttgcgc gagaagacga acagcaacgc cttacctgcg
     5341 gacattcaga aatacgcctc cgacttggca gacacgctaa acaacatcgg catcgtgtac
     5401 caccaaaggt ggcatgcgat gaggggcagg aaccgcgaca aagccgatct attggaggcg
     5461 aataaatatt acgcagcaag catgaagcac gtcgaaggta gggacgccat cagggaagcg
     5521 actacccggc tcaaccaagc cgagttacag gtgttgctgg gggattacaa ggccgccttg
     5581 gtgagcgcgt ccggcgcggc cgacctcggc gagaggttgg gcttcgcgga agtcgtctgg
     5641 cgcagctggc tcgccaaggg ttacgccctt cagggacaac agcagcagga gcaggcgcgg
     5701 caagctttcg gccgcgccat cgaactggtt gaggacttgc gcaggcagtc acccggcgac
     5761 gaggtggaaa ggcagaagtt ctttgaggac aaggtcgcgc ccttccacgc tatggtcggc
     5821 cttctggctg cggagaagaa caagggtgaa gcactcttct actcggagcg cgcgaagggg
     5881 cgtgtcctac tggacgtgtt acaaggcggc aggttacttg tcaacaaggc catgactgcc
     5941 gaggagcagc gtcaagagca ggagtttcaa cgccagttag acctacagga tagagccgtc
     6001 tccgaagcgt ggggaggccg gagagaggga gtagaattta agctactgct cgacaggcgc
     6061 agtaaactgg agcgcgatta tgagagcttc ctgcaaagac tgtatgaggc acaccccgag
     6121 ctgaaggtga ggcgcggcga ggcgcaggcc gtgcgtgccg atgaggtcgt tcgtcttctg
     6181 ccggacgcca gcgctgtgat tgagttcacg gtgacggcag acaagactta tgtcttcgtg
     6241 ctgacgcgct cgcgcggcgc ggcgagcacg caacgggcag gagacgccgc gaacctgacg
     6301 ctgtacacgg tcgatatcac aaaggaggag ttgagggatc gcgtcgcgcg tttccgcgag
     6361 atgataggga gggtagaccc aggttataag aagccggcgg ccgaactctt ccaactcctc
     6421 ctcggccgag cgagcccgca gttgaaggac gttaagacgc tggtggtcgt gccggactct
     6481 tacctctggg aactaccgtt ccaggcgctc cagcgccccg gcgggcacca cctgcttcag
     6541 gacttcgttc tgttatacgc cccctcactg acggtgctcc gtgagatgat gaaccagcgc
     6601 ccgggtgggg acgaggcgat gcgacccacg ctgctcgcgc tagggaatcc aagcctagac
     6661 ggggacgtgc tccagagggt tgaggccgcg agaatggatg cggaactcga accgctgccg
     6721 gacgctgaaa agcaggtcaa cgaattacgc gacgttgtgt acgggcactc acttagcaag
     6781 gtgtatacgg ggccggacgc gcgtgaggac accttcaagg aggaggccgg gcgctatcgg
     6841 attctgcacc tcgccaccca cggtatactt aacaacgccc agcctatgtc gtcttactta
     6901 gttcttgcgc ctccgcgcgc gccgcgcgcg cagggcgacg atgggctgct agaggctagc
     6961 gagctcgcga gactcaacct gcgggcagac ctcgttgtgc tcgccgcgtg cgagacggcg
     7021 cgcgggcagg tgggggaagg cgagggcatg gtcggcatcg cttggtcgtt gttcgtcgcg
     7081 ggatgcccca ctaccgtcgc cagtcagtgg aaggtcaggt cggacagcac acggctgctc
     7141 atgctggaat ttcatcggaa cgtcgtcggc gacttaggca ctcggccgtc gcacatacgc
     7201 gcggcgcggg cgctgcggga agcggcgctg cgggtgttca acgaaaaaga gttagattac
     7261 aaacacccat tccactgggc cgggttcatc gcgatcggcg acggcctatg acccgtccgg
     7321 gcaccggcaa ggtcagacac cgtcaacgcc tctgagacct gagttcgccc agtaacttct
     7381 gcaagacggc agaacccggg ttgtcgcgga gcagtgcctg caattccccc tcagcctgct
     7441 ccggcatgct cgagcgcgcg tagacgatcg ccgccagcag ccgcgagttg tatttacctt
     7501 tggcactccg cagcatctcg gcggcggagc tttccatgac tttaaacttc gtctgtgata
     7561 gccgcctcgc ccgcccgccg gcgtcgtccg gcctgtgcga cgaaacttcg ccaccttctt
     7621 tgaaggccgt caccttccag gagtaggcgt ggccgtgccg tagcttgccc tttggcacct
     7681 gccatttgat cgtgtgcagt tcggggcttt ctgccactgg gttgaattgc tcgtcgaaca
     7741 cttcaacttt gtagcctttg gccttgtcaa ggggattcca cgttagaaca ggggtagtcg
     7801 tggggacgac ctcgccagcg ggactcagga gtgtgaattc cacgtcggcc gaagggctgc
     7861 ccatcacctg cccggcgact tcggtcaact ctgacgggag cggggcgaca cttatttgct
     7921 gcttcgagag ggtcagttcc agcgctgccc tatatgattt cgggagcgtt ttgttaatcc
     7981 ccgacacatt cccctgctcg tctaagacaa aagtattcac gccgtccatg acaacgagcc
     8041 cgaggtcggc cgggagtact ggggcggcat ctttgccggg gagtggtgtc gggctcggac
     8101 ttcgattcgt ccctaccacc ccactctgcc ccggccggtt gtgccccttg tcttcgggcg
     8161 atggaaggac ttgagcgacg atctccttgc gctcccctgg ggtttcgacc gctgtgggtt
     8221 gttcgacggt ctttggtcgc cagaaggcca attcaacttt gcccgtgtag aagacccagg
     8281 ccgccacaga caacaccgcc gcgacgccga agaccgcgac gtaccgcagt gcgagccccc
     8341 acgcccccgg ctgcgctcct gccgtccaag acttgattcg gtcgccgacc gcccaggccg
     8401 cgccccgcag ccagccaaac aaatctcctt ccgagtacgc cacaacgacg ttagcacctc
     8461 gcgccgcgcc cgcgcttgcg ggcaacgggg tcacggtcac gttaacccgc tgcccccctt
     8521 cgagcctgat aacgcctttc cagtcctctt cgccgtccag cgcgccggaa ctactgagca
     8581 gcagcgaccc gagcagcagc ggcccgtcct cgtccgacgc aacaacttga ataaggtcgt
     8641 cgcccctttc gagcgtgaaa gtgacttcac tctgctcggc agggtcgaac gacgttcgct
     8701 ccactccatc gacttcgacc gacaattctg ttcggcgcgc tttcctcctg cggcgtctac
     8761 cgccggtcac ctcatcgaat aacttcctaa gcctatcgac ggtgaactgc ggcagcttgt
     8821 taaagtccac cggcgggatt tgatccggtt ggtcggggag gttgaataac ggcacggcca
     8881 gccgttccac ggggggcggg actttcagtt cccttgtcac cgctgtgaga cagtcagggt
     8941 cgatgatggc gcgcatacgc ttctcctcga acgggctctc cttgtcgggg tggcctgtga
     9001 acctgaagtt cctaaacggg ctcgcactcc tgctgcccgc ctccagagcg cacgccgttc
     9061 cccacggaga caatagcttt aggacgtttt cgaccagccc gacgagttgc tcttgctggc
     9121 cgtgagtctc gaaagacttc gcgccctgct tcccgccttt gaccgtcagc agtcctttga
     9181 aacgggcttc gtgaagctcg ttcatcagtg ccaacttcca gtctttgtac atctggtcgt
     9241 tcttcctgag gcgggggtct tgcgcgaggc tgtcgtggac gaggtagacc tctcttttca
     9301 ggtaatcgta gaggagtctg cccagcgcga cggccacttg gaaggagcgg tggggaacgg
     9361 tcatgtacag gatgtgtttg atgtagcgcg tgacgaaata actttcgtga ggcatcggcc
     9421 cgagtccttc gcgccaccgc gcctcctgcg ccaattcctc tttgtggcag agacgaaagg
     9481 cgatgaactg tagggccagc tcccgcctca taatcagttt ttgccggaag ccggaagcgt
     9541 caatccggta gcgtttgcgg gctcgctgct ccttcacctc gacatccagt gcgagcgcgc
     9601 agttcacggt tgccgcgaac gccgcgtcct cgtccccgag caccgcgtag cagagttgga
     9661 acaccctctc aagttgcgcc gactccggca ctctaccgaa catgtaccac ccatctggcc
     9721 tcgcggcggg aatcctacta ccgcacagct atcagacaca gcacgcaggc cgcaggaagt
     9781 atagcaacgc gaaaaggcaa agaccgtagt tctttaccgt taattatccg cgaattgtta
     9841 cccctctttt ttcgaggcgg ggcggaaaac gacgacggac taaagggctt gggagctgag
     9901 atacttgcct ccgcttcaac agccagcggg gctctaccgc ggcgcaaaac acgaatccgc
     9961 tggtgtgcaa tgcgcgagcg ggcagacaca cgacaaagag cagcaataga gctaacccct
    10021 tctctatgac cttcgcatct gcacggagga tacacggggc aaaaagcaat tggaggtaag
    10081 aaagatgtca gttttgaaca accgtccgag catcaattca gtaaaagcga aggccatctc
    10141 attcacgtgc accgtggctt tattgctttt cttctctgtg gtcaacaccg ttaacgtaca
    10201 tgcgacagta cccgtcccct ccggcttcac ggccatctcg tcgggcacgg gcgtgacggt
    10261 gtaccggaaa aactacacgg gtgggcagcc cgacttcgtc accgtcgtag acctaaggta
    10321 tgcaacgctt cagagctaca cgggctgggc gcaatccgat gggacggtcg agcgccgatc
    10381 gctcaccacc cactggcaga acgcggtcgc ccagaacacc tcgacgcgaa tagcgaaggt
    10441 cgcgatcaac ggcacattct tcgacccgaa catgtcgccc tacacgggga tagcgttcgg
    10501 cctcaaggcc aactggtgga taatgagccg ggggtacgcg gctggtacgg agtaccctgg
    10561 actcatccga accctcacct acgatgcgac ctacaacagc tccagcattc aggcgtacaa
    10621 cagcaccacg ttcgaccagg gaatttccga tgtcgttggc gggctcgacc cgaccgctaa
    10681 taaagacagg tacagcaaca ttccgcgaac cttcgtcggc gtgcgcgacg acaacgggga
    10741 cggccactgc gaaaccgtca tcttcttttc gagcaagtac gccacccaag cctgggcgac
    10801 caatatcctc accggcttcg gcacggtcgc gaggatgatg ctcgacggcg gcgggagcac
    10861 cgggctggtc gttgacggta cgcaacatat ccccacgtcc cgtaccctgc cccacgtatt
    10921 catcgtcagc gccgggaagt agcaaacaca gtaaccagga catccgccgc cgacgctcgc
    10981 ctctgaggat gggctcgctc atcagtcacc cgtcggcccg ggttaccaac tatacaaagg
    11041 agtaacatca tgagatttag ctacaggaaa cgccacttgg ccggggtact ttcgctgtac
    11101 ctagccctgc tgggcggcat cctcgcgccg cagtcgggct ggacacaagc cttcgcggcc
    11161 tcgcccgtcg ccccgcaggt ctatggccct tattacgtgc agagcccggt ctatggcggc
    11221 tggagcggcg acgaaaagtg gtacaacgtg tcgggttcgt ccatgcacaa ggctttcgcg
    11281 acgttcgacg acagcaacgc ctacgacatc aatctgccat cgaaccggga gaacatgttg
    11341 ccggtctact cctccggcta cggcaaggtg acatctctcg gcacgacatt ccctggcacg
    11401 accgacggcg gtacatacgg ggcggtgctg atagaccatc agaacagcac ctacaccggc
    11461 tacatgcaca tgcgcaacat tcaagtgagg gcgggcaaca gcgtcacccc caacacgatc
    11521 atcggctatg tcagcgacaa gggcgtgccg ggggtggttc acctgcactt cgccttttac
    11581 ctgaagatta agaagaacgg caaggactat ctcagctcgg cgagcaccta catcgcaccg
    11641 cgcgacttcg gcctgagcct cccgcggtcg atttcgctca ggcgcggggc atccttccag
    11701 gtttcggcct ccgttccctt ccccagcatc accaacagcc gcgtccaaga ccagctctgg
    11761 tacaacaaca cgtactggac gagcagcaat agccgcgtcg ccaccgtgtc cggcagcggg
    11821 ttggtgaagg cgttggcggc cgggtcgtca acgatcacac tgaagttctc cggcaagacc
    11881 ttcaacattc ctgtcaacgt gaccaactag caccagcatc gacacgggtt gtggggtcgg
    11941 ccgaccccac aacccgcatc gcgcaaattc ctacacgtaa aggcgtttgt a
//
LOCUS       DASWNS010000466         4254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_68882, whole genome shotgun sequence.
ACCESSION   DASWNS010000466 DASWNS010000000
VERSION     DASWNS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68882"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VGB76_16035"
     CDS             complement(<1..617)
                     /locus_tag="VGB76_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100455.1"
                     /translation="MNTKLGVTPATHGAAETPPTETRPATRTEERSSPFRFDQRFIAP
                     AFITLILVVGQFSFGILESYTKTALAIVTSILVELILARLFTGKWPHLASAYISGISV
                     GILVRSPAVWPYALCSAISITSKYVLRVKGRHIWNPSNFGICALLFLAHETVASLSIQ
                     WGNNIWPMLAIWTLGSVIIYRLRHFHITFTYVTSFILFAFLRSWIT"
     gene            complement(614..2416)
                     /locus_tag="VGB76_16040"
     CDS             complement(614..2416)
                     /locus_tag="VGB76_16040"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEY0100456.1"
                     /translation="MKSSFAKVLLVIFFLCLLATPLAIKRYNAWREHSVKTVTPDKSS
                     ALARYGFRLEEVGKASGIDFTHRAPRLDAKLDHIMPQVASMGASVSVVDFDRDGWQDL
                     YVTNSGEDSRNALYRNNRDGTFTDVAPALGVADVNRPGTGVSMGAVWGDYDNDGYEDL
                     FLNKWGRPELFHNDAGKSFTSATTQAKLPQWANANTAVWFDYDRDGLLDLFIGGYYPE
                     SIDLWHLTTTLMMPESFEYAQNGGRKYLFHNLGGGAFEEVSAALGIDSRRWALAAAAA
                     DLRDTGYSDLVVANDYGVTEIFANEGGRRFSNIGEKTGVGFSPKSGMNASFGDILNQG
                     KFAIYVSNISEEGVLIQGNNLWVPKEGTRGNALQYENMARDMGVELGGWSFGAQFGDL
                     NNDGRLDLYLTNGYVSADKGTNYWYDFSKVAGGNSRIISDAANWPPMNGRSLSGFQQK
                     RVWLNDGAGRFNEVAQVVGATDTYDGRSVALVDLWNRGVLDVVVANQRGPLLVYKNTV
                     APDNKWIGFQLEGTTSNRSAIGARVMLYWNGQQQVQEVSGGSGFCAQNMRPLHFGLGQ
                     TAQVEKVEIRWPSGKMQMLAQPGIGQVHQLKEPV"
     gene            complement(2535..>4254)
                     /locus_tag="VGB76_16045"
     CDS             complement(2535..>4254)
                     /locus_tag="VGB76_16045"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HEY0100457.1"
                     /translation="GRLDLVGVKDSGHAARLVNRGAKNYHWQVVRPRAKQASGDQRIN
                     SFGVGGEMEIRSGLLVQKQLITSPSVHFGLGEQTATDVVRIVWPNGSVRAEFALKADQ
                     IIVAEQRLKGSCPFLFAFDGREMKFVKDGSPWGSAIGLRIETLGTAAIQATEEWFKIR
                     GDQLAPRGGFYDLRVTAELWETYYYDHLSLMVVDHPAGTEVFVDERFSVPPPALAIHT
                     TETPQALKRATDDLGQDVTETVRHEDGKYLDTFGRGDYQGITRDHYVEIELPALAVNG
                     NAGDQSSQASQALSATGSAGGSGKAVDEKLWLVAHGWVHPTDSSINEAIGQGQRVRAE
                     GLRIEVPDGRGGWETAEDNLGIPAGRLKTLLFDISNIFRPGTPRRLRLRTNLEIFWDK
                     IEVARGLPRAQPKITRLDASTAELGYRGFSVINQPDASSPELPDYKRLAGTTQRWRDL
                     VGYYTRFGDVRELMAKVDDRYVLMNAGDELRFLFPAQAAPQQGWVRDYILIGDGWIKD
                     GDYNSEFSKTVLPLPSHDRREYTSVAPRLEDDPVYRRFPEDWQRYHTRYVTPDNFRRA
                     LQSTQK"
BASE COUNT          795 a         1264 c         1310 g          885 t
ORIGIN      
        1 gtgatccaac tgcgtaggaa ggcgaagagg atgaacgagg tgacgtaagt gaaggtgatg
       61 tggaaatggc gcaggcgata gatgatgacg gagccgagcg tccagatggc gagcatcggc
      121 cagatgttgt tgccccattg aatgctgagg ctggcgaccg tctcgtgcgc gagaaagagc
      181 agcgcgcaga tgccgaaatt ggaagggttc cagatgtggc gacccttaac tctgagcacg
      241 tatttcgagg tgatggaaat cgcgctgcac agagcgtaag gccagacggc gggcgagcgc
      301 acgaggatgc cgacgctgat gccggagatg taggcgctcg ccagatgcgg ccacttcccc
      361 gtgaagaggc gagccagaat cagttcgacc agtattgagg tgacgatggc gagcgccgtc
      421 ttcgtgtagc tttcgagaat gccgaacgag aactgaccca cgacgaggat cagcgtgatg
      481 aaggcgggcg cgatgaagcg ttgatcgaag cgaaagggag aactacgttc ctccgttcgc
      541 gtcgcaggtc gcgtctctgt gggcggcgtc tctgccgcgc cgtgcgtcgc gggcgtgacg
      601 ccgagttttg tgttcatacg ggttccttca attggtgaac ctgaccgatg cccggttgcg
      661 caagcatctg cattttaccg gaaggccagc gtatctcaac cttctcaacc tgcgccgtct
      721 ggccgaggcc gaaatggagc gggcgcatgt tctgcgcgca gaagccgctg ccgcccgaaa
      781 cctcctgaac ctgttgttgg ccgttccaat aaagcatcac gcgtgccccg atggcgctgc
      841 gattgctcgt cgtgccttcc aactgaaagc cgatccactt gttgtcaggc gcgaccgtgt
      901 ttttgtagac gagcagaggt ccacgctggt tggcgacgac gacatcgagg acacccctgt
      961 tccataaatc gacgagcgcg accgagcgac cgtcgtatgt gtcggttgcg ccgacgacct
     1021 gcgccacttc gttgaatcgt cccgcgccgt cgttgagcca gactcgcttc tgctggaagc
     1081 cggaaaggct gcgcccgttc atgggcggcc agttggcggc gtcggagatg atgcggctgt
     1141 tgccgcccgc gactttcgag aaatcgtacc agtagtttgt gcccttgtct gccgatacgt
     1201 agccgttcgt caggtagaga tcgagccgcc cgtcgttgtt caagtcgccg aactgcgcgc
     1261 cgaaactcca gccgccgagt tcgacgccca tgtcgcgcgc catgttctca tattgcagcg
     1321 cgttccctcg cgtcccttcc ttcggaaccc agagattgtt gccctgaatc aacacgccct
     1381 cttccgaaat gttgctgacg tagatggcaa acttgccttg attcagaatg tcgccgaagg
     1441 aagcgttcat accgctcttg ggagagaagc cgacgccggt tttttcgccg atgttcgaga
     1501 agcggcggcc tccttcgttg gcgaaaatct ccgtcacgcc gtaatcgttg gcgacgacca
     1561 gatcggagta gcccgtgtcg cgcaggtcgg cagcagccgc cgccaaagcc caacgccgac
     1621 tgtcgatgcc gagcgcagca ctcacttcct cgaacgcgcc gccgcccaga ttgtgaaaga
     1681 gatacttgcg cccgccgttc tgcgcgtact cgaaactttc gggcatcatc agcgtcgtcg
     1741 tcagatgcca gagatcaatt gattccgggt agtagccgcc gatgaagaga tcgagcagcc
     1801 cgtcccggtc gtagtcgaac cagacggcgg tgttggcgtt agcccactgc ggtagtttgg
     1861 cctgcgtggt cgcgctcgtg aaggatttgc ccgcgtcgtt gtgaaatagc tcgggacgcc
     1921 cccacttgtt taggaacagg tcttcgtaac cgtcgttatc gtaatcgccc cacaccgcac
     1981 ccatcgaaac gcccgtgccc ggtctgttta cgtcggcgac gccgagcgcc ggtgccacgt
     2041 ccgtgaacgt gccgtcgcgg ttgttgcggt agagcgcgtt ccggctgtcc tcgccgctgt
     2101 tggtgacgta gaggtcttgc cagccgtcgc ggtcgaagtc aacgacggag acgctcgcgc
     2161 ccatcgaggc gacctgcggc atgatgtggt cgagtttcgc gtcgagccgg ggggcgcggt
     2221 gtgtgaagtc gatgccgctc gctttgccca cttcttcgag cctgaatccg taacgcgcga
     2281 gcgcggaaga tttgtcgggc gtcacggttt ttacggaatg ttcgcgccat gcgttgtagc
     2341 gtttaatggc aagcggcgtg gcgagcaggc agaggaaaaa tataacgagt aaaactttag
     2401 cgaaactgct tttcattgcc agctacttcg agcacaactg cccctgatgc gaaaggtttc
     2461 aacccgtgtt gtttaagcgc actttgaacg ggcgtttgct cggtctgtcc tctagaggga
     2521 tgtttacctc acatctattt ttgagtggat tgaagcgcgc gccgaaaatt gtcgggcgta
     2581 acatagcgcg tgtggtatcg ctgccaatct tccgggaacc gacgatagac tggatcgtct
     2641 tcgagacgcg gtgcgacgct agtgtattcg cggcggtcgt gtgatggcaa ggggagcacc
     2701 gtcttggaaa attccgaatt gtaatctccg tccttgatcc acccgtcgcc gatcaaaatg
     2761 taatcgcgca cccacccttg ctgcggcgcg gcctgcgccg gaaacagaaa gcgcaactcg
     2821 tcgcccgcgt tcatcaacac gtagcggtcg tcaactttcg ccatcagttc gcgcacgtcg
     2881 ccgaagcgcg tgtagtaacc gacgaggtcg cgccagcgtt gcgtcgtccc ggcgagacgt
     2941 ttgtagtcgg ggagttccgg cgaagacgcg tcaggctgat tgatgaccga gaaacctctg
     3001 tagccaagct ctgcggtgga cgcatcgagc cgcgtgatct tcggttgcgc gcggggcaag
     3061 cctctggcga cttctatctt gtcccagaat atttcgaggt tggtgcgcag gcgcaggcga
     3121 cgcggcgtgc cggggcgaaa gatgtttgag atgtcgaaca gcagcgtctt caaacgtccc
     3181 gcggggatgc ctagattatc ctcagccgtc tcccacccgc cgcgcccgtc cggcacttca
     3241 atgcgcagac cttcggcgcg aactcgctgc ccctgaccga tggcctcatt gatcgaagaa
     3301 tccgtcgggt gcacccagcc gtgcgcgacg agccacaact tttcgtcaac ggccttcccg
     3361 gaaccgcccg cactccccgt cgcgctcaac gcctgcgacg cctgcgacga ctgatctccg
     3421 gcgttcccat tgacagcgag ggcgggcagt tcgatctcga cgtaatggtc gcgcgtgatc
     3481 ccctgatagt ctccgcgccc aaaggtgtcg agatacttcc cgtcctcgtg cctgacggtt
     3541 tccgtcacgt cctgtccgag atcgtccgtc gcgcgtttga gggcttgcgg ggtttcggtc
     3601 gtgtggatgg cgagcgcggg cggcgggacg gaaaatcttt cgtcgacgaa gacttccgtt
     3661 cccgccggat gatctacgac catgagtgag agatgatcgt agtaataggt ttcccagagt
     3721 tcagccgtca cgcgcaggtc gtagaagcct ccgcgcggcg cgagttggtc gccgcgaatc
     3781 ttgaaccatt cttcggtcgc ctgtatcgcc gccgtgccga gcgtctcgat gcgcaagccg
     3841 atggccgaac cccacggcga gccgtctttg acaaacttca tctcccgccc gtcgaacgca
     3901 aacaggaacg gacaggagcc tttcagacgc tgttcggcga cgataatttg atctgccttg
     3961 agcgcaaact cggcgcgcac cgaaccgttc ggccagacga tacgcaccac gtcggtcgcg
     4021 gtctgttcgc cgagtccgaa gtggacggat ggacttgtga tcaattgctt ttgcaccagc
     4081 aggccggagc gtatctccat ctcgccgccg acgccgaacg agttgatgcg ttgatcgccc
     4141 gacgcctgct tcgcgcgcgg cctcaccacc tgccagtggt aattctttgc gccgcgattg
     4201 acgagccgcg ccgcgtgccc tgagtctttg acgccgacga gatcgagccg tccg
//
LOCUS       DASWNS010000467         4697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_146839, whole genome shotgun sequence.
ACCESSION   DASWNS010000467 DASWNS010000000
VERSION     DASWNS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146839"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /gene="guaB"
                     /locus_tag="VGB76_16050"
     CDS             <1..1427
                     /gene="guaB"
                     /locus_tag="VGB76_16050"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY0100458.1"
                     /translation="LLVPGRSDVLPNETDTSTQFTRGISLRIPLCSAAMDTVTEAALA
                     IAIAQQGGIGVVHKNLPVERQAEEVDKVKRSESGMIVDPVTIRYDRPVSEALAVMERY
                     KISGVPVVDEDGHLVGIITNRDLRFETRFDIPVSEVMTKQPNLVTVPVGTTLEQAKSV
                     LQKHRIEKLLVIDDDKRLKGLITVKDIQKAIKYPFAAKDDLGRLRVAAAIGATGDYKE
                     RADALVAARVDCLIVDTAHGHSTRVIEAVREMRRRHPDTQLIAGNVATGEATRELIEA
                     GVDAVKVGIGPGSICTTRVVTGAGVPQITAIVNCVQAARGSGVPVIADGGVKFSGDVA
                     KAIAAGADVVMIGSLFAGTEESPGELILFQGRSFKAYRGMGSIGAMREGSRDRYAQES
                     VAMESKLVPEGIEGRVPYKGTLSDMVTQLIGGLRAGMGYTGCRTIREFQEKTRFIRIS
                     AAGLRESHVHDVVITKEAPNYRLE"
     gene            complement(1517..2416)
                     /locus_tag="VGB76_16055"
     CDS             complement(1517..2416)
                     /locus_tag="VGB76_16055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0100459.1"
                     /translation="MRRRLFIYALLIVCFTASHARAQQDFSKVQMKATKVAGNIYMLE
                     GAGGNIGVSAGADGILIIDDQFAPLADKIRAALKEINPGQLRFVLNTHYHGDHTGSNP
                     IFGREATIIAHDNVRARLATEQTVRGQKIPASPREALPVITFNQSASVHFNGEEIRLV
                     HFPNGHTDGDSIMFFTASNVVHMGDHFFNGRFPFVDIEHGGSVEGLTRNVEAVIARLP
                     ADVKIIPGHGALSTLDDLKLYRRMLTETTALVRRGMTQGKTLEQLKAAGLPEEWKIWG
                     TGFVNTGFWIETIYNSLSKDGKK"
     gene            2776..3723
                     /locus_tag="VGB76_16060"
     CDS             2776..3723
                     /locus_tag="VGB76_16060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0100460.1"
                     /translation="MTKRRTVRLISNPNAGRGGRRREAQVARFCELLRAREIEVEVKT
                     TSAAGDATRLAAEAAQAGINEVIVSGGDGTINEALQGLIGTRVRLGIWPAGTANVLAR
                     ELQLPFDAAGAAEVIARGATRRIYVGSATDEGSGARRYFFLMAGVGLDASVVERVRPR
                     LKRRVGEGAFWVSGLAHLVRWRPVPFEVEVEGQTYPATFAAFGRAAHYGGDLSVTPRA
                     RLDEPEFEICIVNSRSRLRFLYLLTHALGANGVRREMTFVRYVRATRARATGEARMQV
                     DGELTGRLPTTFEIVPTPVEVIAPALQERDGRRGYQRLR"
     gene            complement(3840..>4697)
                     /locus_tag="VGB76_16065"
     CDS             complement(3840..>4697)
                     /locus_tag="VGB76_16065"
                     /inference="COORDINATES: protein motif:HMM:NF017142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100461.1"
                     /translation="DPTAPRGDGMEHLDSTQIITTRALGDRDVLEDAVDTAAHEFFHV
                     WNVKRLRPVELGPWDFTRPVNTRSLWIAEGLTNYYGGLLQRRAGIWSDERFLRALAGA
                     IGFVESRPGNRLTSAEEASLIASFIDRTPHAQRTNLAETTVSYYSKGEVIGIVLDLLI
                     RGRTRGRASLDDVMRRMYEEFYVRSPATTYYLRGRGYTGADFERVVTQVTGADMSEFF
                     ARHVRGVETLPYEEAFAYVGLRLVKSPASAAQTPNNAQSQARENYRLEELPNAPAEAR
                     ALRAAWLKG"
BASE COUNT          855 a         1436 c         1541 g          865 t
ORIGIN      
        1 ttctcttagt tcctggccgc tccgacgtgc tgccgaacga aaccgacacc tcgacgcagt
       61 tcacgcgcgg catctccctg cgcatcccgc tctgctcggc ggcgatggac accgtgacgg
      121 aagccgcgct cgccatcgcc atcgcgcaac agggcggcat cggcgttgtg cacaaaaacc
      181 ttcccgtcga gcggcaggcc gaagaggttg acaaggtgaa acgctccgag tccggcatga
      241 tcgtcgaccc ggtgacgatt cgctacgacc gccccgtctc cgaggcgctc gccgtgatgg
      301 aacgctacaa gatttccggc gtgccggtcg ttgacgaaga cggacatctc gtcggcatca
      361 tcaccaaccg cgacctgcgc ttcgagacgc gcttcgacat ccccgtctcc gaagtgatga
      421 cgaagcagcc caacctcgtc accgtgcccg tcggcacgac gttggagcag gccaagtcgg
      481 tgctgcaaaa gcatcgcatc gagaagctcc tcgtcatcga cgacgacaaa cggctgaaag
      541 gtctgatcac cgtcaaggat attcagaagg cgatcaaata cccgttcgcc gcgaaagatg
      601 atctcggacg cctgagagtg gccgccgcca tcggcgcgac gggcgattac aaggagcgcg
      661 ccgacgcgct cgtcgcggcg cgcgtcgatt gcctgatcgt ggacaccgca cacggccatt
      721 cgacgcgtgt gatcgaagcc gtccgcgaga tgcgccgcag acaccccgac acgcaactca
      781 tcgccggaaa cgtggcgacg ggcgaagcga cgcgcgaact gatcgaagcg ggcgtggatg
      841 ccgtcaaggt cggcattggt cccggctcga tctgtacgac gcgcgtggtg acgggcgcgg
      901 gcgtgccgca gatcactgcg attgtgaatt gcgtgcaggc ggcgcgcggg tcgggcgtgc
      961 cggtcatcgc cgacggcggc gtgaaatttt ccggcgacgt ggcgaaagcc atcgcggcgg
     1021 gcgcggacgt ggtgatgatc ggttcgctgt tcgcgggcac ggaagagtcg ccgggcgagt
     1081 tgatcctgtt tcagggtcgc tcgttcaaag cctatcgcgg catgggttca atcggcgcga
     1141 tgcgcgaagg ctcgcgcgac cgctacgcgc aggaatcggt ggcgatggaa tcgaagctcg
     1201 tccccgaagg catcgaaggg cgcgtcccct acaagggcac gctctccgac atggtaacgc
     1261 aactcatcgg cggcctgcgc gccgggatgg gctacacggg ctgccgcacc atccgcgagt
     1321 ttcaagagaa gacgcgcttc atacgcatca gcgcggcggg cttgcgcgag tcgcacgtcc
     1381 acgacgtagt gatcacgaag gaagcgccga attatcgtct ggagtaagcg gaagcgacaa
     1441 gactttggct caacaacaaa cgcacggcgt ccgcatcaac cccggacgcc gtgcgctttt
     1501 tgtttgtcga agcggactac tttttgccgt ctttcgacag gctgttgtag atcgtttcga
     1561 tccagaatcc ggtgttgacg aagcccgtgc cccagatctt ccattcttcg ggcagccctg
     1621 ccgccttgag ttgttcgagg gtcttcccct gcgtcatccc tctgcggacg agggcggtcg
     1681 tctccgtcaa catgcgccgg tagagtttga ggtcgtcgag cgtggagagc gcgccgtggc
     1741 cggggatgat cttcacgtct gcgggaaggc gcgcgatgac ggcttcgacg ttacgcgtca
     1801 aaccttcgac gctgccgccg tgttcgatgt caacgaacgg gaagcgaccg ttgaagaaat
     1861 ggtcgcccat gtgcacgacg ttcgaggcgg tgaagaacat gatgctgtcg ccgtccgtgt
     1921 ggccgttcgg gaaatggacg aggcgaatct cctcgccgtt gaaatggacg gaggccgatt
     1981 ggttgaaggt gatgacgggc agagcctctc tgggcgaggc ggggattttc tggccgcgca
     2041 ccgtctgctc cgtggcgagc cgggcgcgga cgttatcgtg cgcgatgatg gtggcctctc
     2101 tgccgaagat aggattgctg cccgtgtggt cgccgtgata gtgcgtgttc aggacgaagc
     2161 gcagttggcc ggggttgatc tctttgagcg cggcgcgaat cttatctgcg agcggcgcga
     2221 actgatcgtc gatgatgagg atgccgtccg cgcccgcgga gacgccgatg ttgccgcccg
     2281 cgccttcgag catgtagatg ttgcccgcca ctttcgtcgc cttcatctgc acctttgaaa
     2341 agtcctgttg cgcgcgggcg tgagacgcgg tgaagcaaac gatcagcaag gcatagatga
     2401 acaagcgacg acgcataaaa tttccctccg caaatttcta actggttgat gagaacacgc
     2461 cgcgcaaacc cgcgctttcg agcctttgct catcaggcga atttgccaca cggcgatgcc
     2521 gcacagtaac cttttcacgc atcaagcgtc aacatgtctc aatcgggtag cgcgagtgcg
     2581 tgcagggact tgtagtcgct gccgtgcgag gcgttatttc gtgcctgaat catccgacac
     2641 gtgtgcggcg aagcatgcgt cgccgtgact tgcgcgccgc gcgttccgtc taccggcact
     2701 cgatcaagtt ggcattaacg cgtgagactc cggcatcgaa gcgggcggca ttgtgtttgc
     2761 aatagcagag ctgtgatgac gaagagacga accgtaagat tgatctccaa cccgaacgca
     2821 gggcgcggcg ggcgcaggcg tgaggcgcag gttgcgcgct tctgcgagtt gctgcgtgcg
     2881 cgcgaaattg aagttgaagt aaagacgacg agcgcggcgg gcgatgccac gcggctcgcg
     2941 gcggaggcgg cgcaagcggg cattaacgag gtgatcgtct ccggcggcga tgggacgatc
     3001 aatgaagcgt tgcagggtct gatcggcacg cgcgtgcggc tcggcatctg gcctgcgggc
     3061 acggccaacg tgctcgcgcg cgaactccaa ctgcctttcg acgcggcggg cgcggcggaa
     3121 gtgatcgcac gcggcgcgac gcgacgcatc tacgtgggca gcgcgacgga cgaaggcagc
     3181 ggcgcgcgcc gttacttttt tctgatggcc ggggtggggc ttgacgcttc ggtggtcgag
     3241 cgcgtgcgcc cgcgtctcaa gcggcgcgtg ggcgaaggcg cgttctgggt ttcaggactc
     3301 gcgcatctcg tacgctggcg acctgtgccc ttcgaggtag aggtcgaagg gcagacgtat
     3361 cccgccacct tcgccgcctt tggccgcgcc gcgcattacg gcggcgacct cagcgtcacg
     3421 ccgcgcgcac gcctcgacga accggaattc gaaatctgca tcgtcaactc acgcagccgc
     3481 ctgcgcttcc tgtacctgct cacgcacgcg ctcggcgcaa acggcgtgcg gcgcgagatg
     3541 acgttcgtgc gttacgtgcg tgcgacgcgc gcacgcgcga cgggcgaggc gcgcatgcag
     3601 gttgacggcg aactcacagg ccgactgccg acgactttcg agatcgtccc cacgcccgtc
     3661 gaagtcatcg cgcccgcgct tcaggagcga gacgggcggc gcggctacca acgcctacgc
     3721 tgaactttca acttgcacta aaaaattgaa ccacgaaaac acacgaagca acacgaaaag
     3781 tggtcagtgt tgcttcatgc gttttcgcgg tcactgctta ttgttagatg ctctgagttt
     3841 cagcccttca accatgccgc gcgcagggcg cgtgcttccg ccggagcatt gggcagctct
     3901 tcgagtcggt aattttcgcg cgcctgcgat tgcgcgttgt tcggcgtctg cgccgcgctc
     3961 gcgggactct tgacgagccg caggccgacg taagcgaacg cttcttcgta aggcaacgtt
     4021 tcgactccgc gcacgtggcg cgcgaaaaac tcagacatgt ctgcgcccgt cacctgcgtc
     4081 acgacgcgtt cgaaatccgc gcccgtgtag ccgcgcccgc gcaggtaata ggtcgtcgcc
     4141 ggactccgca cgtaaaactc ttcatacatg cgccgcatca cgtcgtcgag cgacgcgcgc
     4201 ccgcgcgtgc gcccgcgaat caacaggtcg agcacgatgc cgatcacctc gcctttgctg
     4261 taatagctca cggtagtttc ggcgagattg gtgcgttgag cgtgcggcgt gcgatctatg
     4321 aaggaagcga tgagcgaagc ctcttccgcg ctcgtcaggc ggttgccggg tctgctctcg
     4381 acgaagccta tcgccccggc cagtgcgcgc aggaaacgct cgtcggacca gataccggcg
     4441 cgtcgctgca acaggccgcc gtagtagttc gtcaaccctt ctgcgatcca caggctgcgc
     4501 gtgttcacgg ggcgcgtgaa atcccagggg ccgagttcga cggggcgcag gcgtttgacg
     4561 ttccagacat ggaagaactc gtgcgcggcg gtgtccacgg catcttcgag cacgtcgcgg
     4621 tcgccgagag cgcgcgtcgt gatgatctgc gtggagtcga gatgctccat gccgtcgccg
     4681 cgcggcgcgg tcgggtc
//
LOCUS       DASWNS010000468        18625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_17830, whole genome shotgun sequence.
ACCESSION   DASWNS010000468 DASWNS010000000
VERSION     DASWNS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18625
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17830"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="VGB76_16070"
     CDS             <1..1469
                     /locus_tag="VGB76_16070"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0100462.1"
                     /translation="RGEVTKMSKQGRKSVAFVLALVLMSGQLSCRNPFSSRGEGGATG
                     TPSPHFEPGFNLFSPEQDIELGRRSAQQIAQQMPLLNDEPIVNYVRGLGAKLAANASG
                     HKFPYQFNVVATKELNAFALPGGFIFINAGAIAAAKNEGELAGVIAHEITHVALRHGT
                     NQASKAYIAQKGLGVIGAITGDDSDLGQVLNSIGGAGANMLFLRFGRTAEKQADLEGA
                     RIMAESGYDPRDMANFFKTLQSAGGQRTPEFLSDHPDPGNRVAAISKALPNLRVSNQP
                     IRDTEAFNGVKARLEGRRAVGSLASTAEPARTGPSDPTSNRSGAARPAPPAPSFQPFR
                     ASDDSFAFQYPANWDALSSGDDGDAQMIFAPKGAYGKLGDALVVTHGIFIGAVAPASS
                     DLARANEIYVQQQLDINSDFRVQREPQRISIGGREGYATMVAGPSVVTGVTEVDIIYT
                     TATADGRLFYLITMAPEDEFESYQPAFQQIINSLQLAR"
     gene            complement(1704..1910)
                     /locus_tag="VGB76_16075"
     CDS             complement(1704..1910)
                     /locus_tag="VGB76_16075"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEY0100463.1"
                     /translation="MKSVSGKEFARLLERHGWSLLRVQGSHHVYGKAGSNIRLSVPIH
                     GNQTLKIGLLKHLMKLAGLSETDL"
     gene            complement(1907..2110)
                     /locus_tag="VGB76_16080"
     CDS             complement(1907..2110)
                     /locus_tag="VGB76_16080"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY0100464.1"
                     /translation="MKIKVIVHEAEEGGYWAEVPSIPGCATQGETFEELLQNLYEAVE
                     GCLSVDVSPTEIQERDRVLEIAV"
     gene            complement(2143..4218)
                     /locus_tag="VGB76_16085"
     CDS             complement(2143..4218)
                     /locus_tag="VGB76_16085"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEY0100465.1"
                     /translation="MKRAFAFLLSLSFLLSPQPVRADEGMWTFDNPPLKQWKERYNFE
                     PTPAWLEHVRLASVRLQDGGSGSFVSGNGLVMTNQHVASGQLAKLSTKERDLAKEGFY
                     ARTSAEELKCPDLEINVLVSYEDVTKRVQGAVKANASDKEANEQRKAEMAAIEKESTE
                     QTKFKSDVVTLYSGGEYWLYRYKKYTDVRLVFAPEEQIAFFGGDYDNFTYPRYNLDVT
                     FLRVYENNQPAKTEHFFKWSAKGPSDGEFVVLSGNPGSTNRLLTNAQLQYQRDLGNPL
                     QMQVWSSRRAALNRYSALGAEQARRAGSTRRSLENSIKRLESQQAGLLNARLMGKKEA
                     EEKALRAAIAANPEWQKAYAPAWDQVATAYAALPKYAKRIAFSNLSTSRLGSLASTLV
                     RYAEEIRKPNATRFDEFRDARLEALKVNLFSPAPIYPDMEEAALAAWLEEGQKTLGND
                     DPFIRAVLNGSTPAALAKQLITGTKLADVNVRKSLFEGGADAIAKSDDPLIVVARRIE
                     PIYRELRAWNEENILSVDASAGQKIAAARFAVYGKNIHPDATFTLRLSYGRALGYSED
                     TTLVPFKTTFYGLYDRAEGFGEKPPYDLPARFKEGRSKFDLSTPFNFVYTADTIGGNS
                     GSPVINRNAEVVGLNFDSNIQKLPNRYVYVDEEEGGRAVAVHSMAIIEALRKLYDAEK
                     LAEEITGGR"
     gene            complement(4380..4784)
                     /locus_tag="VGB76_16090"
     CDS             complement(4380..4784)
                     /locus_tag="VGB76_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0100466.1"
                     /translation="MDEANRVQPIILLVEEDDETRPILRRKLQKDGYRLLLAVDEADA
                     LDRLDGGQARPDLILINLIGKTPGEVLQIGRRIREHGKYDGQTPLVVMAEKYGADLEG
                     QDINVGSNDWITYPEDAEQLHRLLARLTTHAR"
     gene            4935..5645
                     /locus_tag="VGB76_16095"
     CDS             4935..5645
                     /locus_tag="VGB76_16095"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0100467.1"
                     /translation="MPLQEVDPKMNRLLACLPENELQSLLPHFESVSLAHGDHVIVPG
                     VPIQYMYFPLNALLSLVTTMEDGSSVESGVVGREGVTGIPVLLNAEQTTMPTFTQVPG
                     VALRVKSAVMKEAYDRRGALHTLLNRYMHTVVVMGSYSTACNALHKIEPRMAKWLLMS
                     SNGVGLNAFNLTHEFLAVMLGVRRASVSEIASTLRRDGLIDYTRGHIKILDRRGLEAA
                     SCECYGKYKDEYERLFSG"
     gene            5669..5866
                     /locus_tag="VGB76_16100"
     CDS             5669..5866
                     /locus_tag="VGB76_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100468.1"
                     /translation="MASKERAQEELKATARKVAREKIMGELREPLSKLGEKRAELKRL
                     RAKLDELKKVTGKLSAAPSKE"
     gene            complement(6030..6428)
                     /locus_tag="VGB76_16105"
     CDS             complement(6030..6428)
                     /locus_tag="VGB76_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100469.1"
                     /translation="MALPATYESASVRNTERGSIEGSTAILPFGLFELDPAGTVIRYS
                     PAAENTQTIEARDVWGRNFFTEIAPVEQVVGFRLRFHSFMASGQAVERFTTTFAHGEQ
                     NIKVQIMLAHITEKSDDGRKRLALVRIMPD"
     gene            complement(7142..7546)
                     /locus_tag="VGB76_16110"
     CDS             complement(7142..7546)
                     /locus_tag="VGB76_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100470.1"
                     /translation="MPEQQNRRTIFLIEEDDDTRPLLKHNLQVDGYRVLLALDEEDAL
                     ARMDGHSAHADLVLINLVGKPLEAVLATGRKIREHAKYDGQTPLVVMAEKYGADLEGQ
                     DINVGNNDWITYLEDHEQLKNLLARLLPEPAV"
     gene            7805..8536
                     /locus_tag="VGB76_16115"
     CDS             7805..8536
                     /locus_tag="VGB76_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0100471.1"
                     /translation="MTTPDGSSLPTTNRILAELPTEEYERLSPHLEPVTLAVGDMLYY
                     PQEPLTHVYFPNRGTVSVIATLADGGGVEVGVVGNEGLFGLNVVLGSMSTPHEAMVQL
                     PGDAFRIPAHVIMGEFKKGGQLQDLILRYVQAFIIQIAQTAACNRSHPMGGRLARWLL
                     MTHDRAMTDELLLTHEFIAVMLGTRRAGVSEAAGRLQNEGLIKYRRGHIQIIDRKGLE
                     STSCECYPLVKREFDRLLGGNGGIH"
     gene            8804..9550
                     /locus_tag="VGB76_16120"
     CDS             8804..9550
                     /locus_tag="VGB76_16120"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0100472.1"
                     /translation="MSELQNARPPTENRILRALPDEEYERLAPHLEFIETTHGQILYQ
                     TQQTIDYVYFPNKAMISLVSQMADGGSIEVGIVGFEGMAGLSFVLGVDRSPYETMVQI
                     HNGAARMPARLLKEEFKRGGTLQDVLLRYTQSLLLVTSQVAGCNRAHSLEERFARWLL
                     MSHDRCLCDNLPLTHEFLSMMLGVRRAGVTTAALALQAEGFIHYTRGHIRIISREGLE
                     RIACECYATLKADFDIVTSGTKNAGGGKPE"
     gene            complement(9657..13472)
                     /locus_tag="VGB76_16125"
     CDS             complement(9657..13472)
                     /locus_tag="VGB76_16125"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0100473.1"
                     /translation="MTQLIIAGLVLLCLLSVGVSAYLARALARVRREQEARQELPVAH
                     AATLAPALPVDPSVEQRVVPLEGASQELETSQPTDAASLAEAFPAEEASADSVEQATA
                     DVVAASSTIDETDVYALASAMDAHYQQSSRPAELRHHEYFHKGVELLKDEKYSTHDLI
                     ELARGTNVVVACIALEALGQRRDASDVVTPLLAHINEFYYWPGYYALRALDARAGERP
                     VIVPLLLHIKGAWGEYTPLQSLREFLTRRIACGETGATFNDALYTLSPDQIELLTTVF
                     SDLRDVLPQSLLREFDEWKEARVDLEFLGSFGRVWKPEVEGAADGKASGANGDAPEGV
                     LMLEAVRERVLEMETALRKEPPRSVLLVGDSGTGKTALVRVLARRLQQERWIILEASA
                     ADVLAGQQYIGQLEGRVQTLVREIGGKRVLWVVPNFHELVWAGRHQYNPSGLLDLLLP
                     HIESGAVKLVGETPSAAYEKLMQLRPRLRTVLETCRVNPLGDAETLEVARRWAMQQHA
                     GGANGDTAARQGFDATETHAAAVTNAAAPRISEQTLQEAFQLAKQYLADKAAPGNLLL
                     LLDSTHRRLLIERDNATVAVATKEPGAEHVRGTANEVTLDDLLVTLSQLTGLPVSILD
                     DRAGLDLKELRGFFEGRVLGQPEAVECLVERVAMIKAGLTDPTRPQGVFLFVGPTGTG
                     KTEIAKTLAEFLFGSPERMIRLDMSEFQTPETLDRILGEPRDTTAESTALVNSIRKQP
                     FSVVLLDEFEKAHPQVWDLFLQVFDDGRLTDRRGNTADFRHCVVIMTSNLGATLPHGA
                     SIGFSQEQAQFASGAVERAVVRAFRREFVNRIDRVVVFRPLGRSIMRDLLRKELNDVL
                     RRRGLRMRQWAVEWDESAIDFLLEKGFTADLGARPLKRAIERYLLSPLALTIVNHQFP
                     EGDQFLFVRAASGGARLDVEFIDPDALEFSGDEPRATTRALAEEDAPDEETSTDEREL
                     RLEAIVSDARGTLAEVQFLQDKFDALADQVEGEEWEGRKTSALLETSAPGFWNSPARF
                     GVLGLAEYMDRIETGLDTAGSLLKRLTGAHTRGRQHFPADLVARLAHQLYLIDAAARG
                     LAAGHPRDAFLSVRASCDAGADASVCNEFARRIAGMYRLWAEARRMRFEILEETKDDA
                     APADATATSAADAPYQMLAAVSGFAAYSLLHGESGWHVLETPQDEKSFNRARVHVRVV
                     AQPDEPAGREGVRGQAARALADGANNAQTQIVRRYREEPSPLVRDSVRGWRTGKLARV
                     LEGGFDLMF"
     gene            13890..14306
                     /locus_tag="VGB76_16130"
     CDS             13890..14306
                     /locus_tag="VGB76_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100474.1"
                     /translation="MTLLSCVSCALLLLACGGASNSNNAPATNAPAANTNAGKASATN
                     TAAPASTAPASNAGSSGDTIGVPECDDYLAKYETCVSSKVPEAARAQYQSTLAQTRKS
                     WRDLAANPQTKASLAQACKTATESARQTMKSIGCEF"
     gene            14626..16626
                     /locus_tag="VGB76_16135"
     CDS             14626..16626
                     /locus_tag="VGB76_16135"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY0100475.1"
                     /translation="MTAEKIARRPAQIKFDGRILFLTDDTTLIRRQLEAGEDLPFDPE
                     RPLMNNISTDEITPGWVCFYYDETLGQYVYVGMREAAVQKDEVKNGGFKVVVSGLSKG
                     CGSSRETAPYAERAAGVELVIARTIEKIYGQNSQNIGLLTSTDFGLIERIRRGEEIPL
                     AEFTRGLDPISQAIVEYGGLFNYNKARLAGETVPPPIETKKRPMNIVEKIIARHAFVR
                     AGEVGVEAVRPGDALFAVADVRFSHEYVTPMAESLFKGALGQDAQVTEAESVYAFRDH
                     LTFLGRVMSDDKKKMGLLERADGLAVTQESFTREQGIKLYSGTEATGSEAICHNAVVE
                     DIALPGQLVIGTDSHTCMAGVLGCFAFGVGSTDMANAWYTKDIRIKVPETVRFVLRGQ
                     KRADVSAKDVMLKILSTDYMKEGRGIGQVLEFAGEGLAEMSIDERATLTNMAVEAGGT
                     TGIIEPDEVTLQYLVNSRGLDAEEIRQGFLKSDPDAEYAATFEINLDEIRPMVATPGD
                     PRNGININELTEEVKIDAAYGGSCTGGKMADMDMYAAVLQRAVEQGRRVAPGVRLYLQ
                     FGSQKIKQYARERGYIEIFERAGAELIDPSCGACINAGPGASKTAETVTVSAQNRNFP
                     GRSGPGKVYLASPYVVAASAIAGRIVEPEEFLQEQAEELVGA"
     gene            16623..17204
                     /locus_tag="VGB76_16140"
     CDS             16623..17204
                     /locus_tag="VGB76_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100476.1"
                     /translation="MKGIGKGHLSADHSPKYERLERERRYLLRELPPGLKVNDQHSQI
                     TDNYITGTRLRLRKVRWPLTNEWTLKLTQKHAPAPPDFSRTLITSIYLNEYEYEVFSV
                     FEANELRKNRYPYEHEGRKYSIDVFLGALRGLILAETDFDTDAEMDTFPPPPFAALDV
                     THDELFTGGRLVYLTIDELRAEFKARVEKWEGR"
     gene            17204..17425
                     /locus_tag="VGB76_16145"
     CDS             17204..17425
                     /locus_tag="VGB76_16145"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEY0100477.1"
                     /translation="MSGRSSGLFVVVVGFVIVGLGLLMWAGALSWFGRLPGDLRFGGE
                     RTRVFIPITSMLIVSLLLSLIMWVVRRFF"
     gene            17437..17931
                     /locus_tag="VGB76_16150"
     CDS             17437..17931
                     /locus_tag="VGB76_16150"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY0100478.1"
                     /translation="MPDNSSPVIYTVGHGRHAWADFLALLREHEIALVVDVRSAARSR
                     WPQFNGRVLEANLREARIGYEWLPECGGKVVAPPPELARGLERIMELASEVRVALMCS
                     ESQPLTRHTQPRANCHRVGLLSTPLRARGARLIHILPDGDTLEIDEAALPSIWKEETL
                     FGDS"
     gene            complement(18044..>18625)
                     /locus_tag="VGB76_16155"
     CDS             complement(18044..>18625)
                     /locus_tag="VGB76_16155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0100479.1"
                     /translation="SFALFTMYRYGYDASHNGYLFMYVGIIGAIIQGGLIGRLVKMFG
                     EMFLVIVGALLFTLSLFAIPLLGQGAGLPLLLLVGGTFALGNSLATPSLQTLASRSVG
                     RGEQGGVLGVVQSVASLARAVGPMIAALLINSAVATYGADGRLHEMSDRSLLVTFWTA
                     AAIMLLAFLLATYFARAHADAYAHTEFPTPAEG"
BASE COUNT         3559 a         5744 c         5713 g         3609 t
ORIGIN      
        1 tgaggggaga ggtgacgaag atgtcgaagc aggggcgaaa gagcgtggcc ttcgtgctgg
       61 cgttggtgtt gatgtcgggt cagttgagtt gccgcaaccc gttctcaagt cgcggcgaag
      121 gcggcgcgac gggaacgccc tcaccgcatt tcgaacccgg cttcaacctc ttcagccccg
      181 aacaggatat cgaactcggc agacgctcgg cgcagcagat cgcgcagcag atgccgctct
      241 tgaacgacga gccaatcgtc aactacgtgc gcgggctggg cgcgaaactg gccgcaaacg
      301 cctccggcca caagttccct tatcagttca acgtcgtcgc gacgaaagag ttgaacgcct
      361 ttgcgctgcc gggcggtttc atcttcatca acgcgggcgc gattgcggcg gcgaagaacg
      421 aaggcgaact cgcgggcgtc atcgcgcatg agatcacgca cgtcgctttg cggcacggaa
      481 cgaatcaggc gtcgaaagcc tacatcgcgc agaagggctt gggcgtcatc ggcgcgatca
      541 cgggcgacga ttccgacctc gggcaagtgc tcaactccat cggcggcgcg ggcgcaaaca
      601 tgctcttcct gcgcttcgga cgcaccgccg agaagcaggc cgacctcgaa ggcgcgcgca
      661 tcatggccga gtccggctac gacccgcgcg acatggcaaa cttcttcaag acgcttcaat
      721 ccgctggcgg ccagcgcacg cccgaatttt tgagcgacca tccagacccc ggcaaccgcg
      781 tcgccgcgat ctcgaaggcg ctgccgaact tacgcgtgag caaccagccg atccgcgaca
      841 cggaggcttt caacggagtc aaagcgcgtc ttgaaggacg ccgcgccgta ggctcgctcg
      901 cctcgaccgc cgaacccgcg cgcaccggac caagcgaccc aacgagcaac cgttccggcg
      961 cggcgcgtcc cgccccgccc gccccgtcct ttcagccttt ccgcgccagc gacgactctt
     1021 tcgctttcca gtaccctgcg aactgggacg cgctctcctc cggcgacgac ggcgacgcgc
     1081 agatgatctt cgcgcccaaa ggcgcatacg gcaaactcgg cgacgcgctc gtcgtcaccc
     1141 acggcatctt catcggcgcg gtcgcgcccg cctcaagcga cctcgcacgc gccaacgaaa
     1201 tctacgtgca acaacaactc gacatcaact cggacttccg cgtccaacgc gagccgcaaa
     1261 gaatcagcat cggcgggcgc gaaggctacg ccacgatggt cgcggggccg tcggtcgtca
     1321 cgggcgtcac cgaagtcgat atcatctaca cgacggcgac cgccgacggg cggctcttct
     1381 acctcatcac gatggctccc gaagacgaat tcgaatccta ccaacccgcc ttccagcaga
     1441 tcatcaacag cctccaactg gcgcgctaaa aatttagcgg cggatgacat gtcgaagtca
     1501 tccgccgctt ttgttgaagg ttgatgagaa gagaagggcg ctcaatgtga gcgtcccgtt
     1561 tgacgaattg ttagagacta acggcgacgc tctttgtgcg cctccgcgta atcccttcgt
     1621 cgcttatcgc gtcaacgcgc gtccagttgc tctctgacag tttcttcaac tttgtatcgt
     1681 aatccgtagc gttgagcaaa gacttacagg tcagtctcgg aaaggccagc tagcttcatc
     1741 aagtgtttta ataaacctat cttgagagtt tggttgccat gaataggaac ggaaagccgg
     1801 atgttgcttc cggctttccc gtaaacatga tggcttcctt gcacgcggag caaagaccag
     1861 ccgtgacgtt caagcaacct ggcgaactct ttaccggaaa ccgacttcat acggcgattt
     1921 ccaatacgcg atcacgctcc tgtatttctg tcggcgacac gtcaaccgat aagcagcctt
     1981 cgaccgcttc gtagagattt tgcagcaact cctcgaacgt ctcgccctgc gtagcgcagc
     2041 ccgggatgga cgggacttcc gcccagtagc ccccttcctc cgcttcatgc acgataactt
     2101 tgatcttcat gttttcgctt ccttgctgcg ccgcaaatta ctttaacgcc cgcccgtgat
     2161 ctcttcggcc agtttctccg cgtcgtagag tttgcggagg gcttcgatga tcgccatgct
     2221 gtggacggcg acggcgcggc ctccctcttc ttcgtcaacg tagacgtagc ggttggggag
     2281 tttctggatg ttgctgtcga aattcagccc gacgacttcg gcgttgcggt tgatgacggg
     2341 cgagccggag ttgccgccga tggtgtccgc cgtgtagacg aagttgaagg gcgtggagag
     2401 atcgaatttg ctgcgcccct ctttgaagcg cgcgggcaga tcgtagggcg gcttttcgcc
     2461 gaagccttcg gcgcggtcgt agaggccgta gaaggtcgtc ttgaagggga cgagcgtggt
     2521 gtcttcgctg tagccgagcg cgcgcccgta ggagaggcgc agggtgaagg tggcgtccgg
     2581 gtggatgttc ttgccgtaga cggcgaaacg cgccgcagcg atcttctgtc ccgcgctcgc
     2641 atccacgctc aggatgttct cttcgttcca tgcgcgcagt tcgcgataga tgggttcgat
     2701 ccgtcgtgcg acgacgatta acggatcgtc tgatttggcg atggcgtctg cgccgccttc
     2761 aaagaggctc ttgcgcacgt tgacatcggc gagcttcgtg cccgtgatga gttgtttggc
     2821 gagcgcggcg ggcgtcgagc cgttcagcac ggcgcggatg aaggggtcgt cgttgccgag
     2881 agtcttctgt ccctcttcga gccacgcggc gagcgcggcc tcttccatgt cgggatagat
     2941 gggcgcgggc gagaagagat ttactttgag cgcttcgagg cgcgcatcac gaaattcgtc
     3001 gaagcgtgtg gcgttcggtt tgcgtatctc ttccgcgtag cgcacgaggg tcgaggcgag
     3061 cgagccgagg cgcgaagtcg agaggttgga gaaggcgatg cgcttggcat acttcgggag
     3121 cgcggcgtag gccgtcgcca cctgatccca ggcaggcgcg taagccttct gccattcggg
     3181 gttggccgca atcgcggcgc gcagggcttt ctcttccgcc tccttcttgc ccatcaaacg
     3241 cgcgttcagg agtccggcct gctggctctc cagacgcttg atcgagtttt cgagcgagcg
     3301 gcgcgtcgaa ccggcgcggc gtgcctgttc cgcgccgagc gcgctgtagc gattgagggc
     3361 ggcgcgacgc gaagaccaga cctgcatctg caaggggttg ccgaggtcgc gttgatattg
     3421 aagctgcgcg ttggtgagca ggcgattggt cgagccgggg ttgccggaga gcacgacgaa
     3481 ctcgccgtcc gaggggcctt tggctgacca cttgaagaag tgttcggtct tcgccggttg
     3541 gttgttttca tagacgcgca ggaacgtcac gtcgaggttg tagcgcgggt aggtgaagtt
     3601 gtcgtagtcg ccgccgaaga aagcgatctg ttcttcgggc gcgaagacga ggcgcacgtc
     3661 cgtgtatttc ttgtagcgat agagccagta ttcgccgccg ctgtagagcg tgacgacatc
     3721 gctcttgaac ttggtctgct cggttgactc tttttcgatg gcggccatct cggccttgcg
     3781 ctgctcgttc gcctccttgt cggaagcatt cgccttgacc gcaccctgca cgcgtttcgt
     3841 cacgtcctcg taggagacga gcacgttgat ctcaaggtct ggacatttca actcttcggc
     3901 ggaggtgcgc gcgtagaaac cctctttggc gaggtcgcgt tccttggtcg agagtttggc
     3961 gagttggccg gaggcgacgt gctggttcgt catcacgagg ccgttgccgg agacgaacga
     4021 accgctcccg ccgtcctgca aacgcacgga ggcgaggcgg acgtgttcga gccaggcggg
     4081 cgtcggctcg aagttgtaac gctccttcca ttgtttgagc ggcgggttgt cgaaagtcca
     4141 catgccttcg tcggcgcgca cgggctgagg cgacagcagg aaggacaggg acagcaaaaa
     4201 ggcgaaggct ctcttcatgg ttctactccg ttcgggtttc agtaagattt cagagggaga
     4261 tataaaactg gattcgtgaa cggaggatat tataaccgac aacctcccgc caacgagaac
     4321 gacgcgggcg cggttcggcc tcgccggaag gtgtgtcttg aagtgccggg gagccggtgc
     4381 tatcttgcgt gggtggtgag gcgcgcgagc agccggtgca gttgttcggc gtcttcgggg
     4441 taggtgatcc agtcgttgct gccgacgttg atgtcctgac cttcgaggtc tgcgccgtac
     4501 ttctccgcca tcacgacgag cggcgtctgc ccgtcgtatt tcccgtgttc gcggatgcgc
     4561 cgtccgatct gcaaaacttc gccgggcgtc ttgccaatca gattaatgag aattagatcg
     4621 gggcgcgctt gtccgccgtc cagacggtcg agggcgtccg cttcgtccac ggccagcagc
     4681 aggcgatagc cgtctttctg tagctttctg cggaggatgg ggcgtgtttc atcgtcttct
     4741 tcaaccagaa ggatgatcgg ctgcactctg ttcgcttcgt ccatgtgtgt tgtgcctgat
     4801 ttgcgctgga cattattgcg cagagaggcc atttaagacg gtacgacaac gaacagaagg
     4861 actggctcag cttgtcttaa actggtattt ctggatttaa aataatgccg tgagcggaaa
     4921 gaaagatacc tctcatgccc ctgcaagaag ttgatccgaa gatgaacagg ctgctcgcct
     4981 gtctgccgga gaacgaactt caatctctgc tcccacactt cgaatcggtg tcgctcgcgc
     5041 acggcgacca cgttatcgtt cccggcgtcc ccatccagta catgtatttc ccgctcaacg
     5101 cgttgctctc gctggtgacg acgatggaag acggttcctc ggtcgagtcg ggcgtcgtcg
     5161 ggcgcgaagg ggtgacgggc atccccgtgc tgctcaacgc ggagcagacg acgatgccga
     5221 ctttcacgca ggttcccggc gtggctctga gggtcaagtc ggcagtgatg aaagaggctt
     5281 acgacaggcg cggcgcgctg cacacgctcc tgaatcgtta catgcacacg gtcgtagtca
     5341 tgggttcgta ctcgaccgcc tgcaacgccc tgcacaagat cgaaccgcgt atggcgaagt
     5401 ggctcctgat gagcagcaac ggcgtcggct taaatgcgtt taacctgacg cacgagtttc
     5461 tggccgtgat gctcggcgtg aggcgagcca gcgtgtcgga gatcgccagc acgctgcgcc
     5521 gcgacggttt gatcgactac acgcgcggcc atatcaagat tctcgaccgg cgcggactcg
     5581 aagcggcttc gtgcgaatgt tacggcaagt ataaggatga atatgaacgc ctgttcagcg
     5641 gctgaaaaac gggcggggga gagagcaaat ggcgagcaag gagcgggcgc aggaagagtt
     5701 gaaagcgacg gcgcgcaagg tggcgcgcga aaagatcatg ggcgaactcc gggagccttt
     5761 gtcgaagttg ggggagaaac gcgcggagct taagcgtctg cgcgcgaagc tggacgaact
     5821 gaagaaagtg acgggcaaac tctccgccgc tccctcgaag gagtgaggcc gaaagttgcc
     5881 gcccgtcgcg tttgagcgtc tccgtatccg gccacgctca agcgaaaccg gccttagtcg
     5941 ttagccgtga gacacgagag gcggcgcgtg cctgtgcagc acacgccgcc tctgctcttt
     6001 aactcgcggc acgatccttc gtctcaagct caatcgggca tgatgcgcac gagcgcgaga
     6061 cgcttgcgcc cgtcgtcgga cttctccgtg atgtgcgcca gcatgatctg cactttgatg
     6121 ttttgctctc cgtgcgcgaa ggtggtggta aagcgttcta ccgcctgccc gctcgccatg
     6181 aaagagtgga agcggaggcg aaacccgacg acctgttcga cgggcgcgat ctccgtgaag
     6241 aagttgcgtc cccagacatc tctggcctcg atggtctgcg tgttctcggc agcggggctg
     6301 tagcgaatga ccgtgcccgc cgggtcgagt tcgaagaggc cgaagggaag gatcgcggtc
     6361 gagccttcga tgctgccccg ctctgtgttg cgcacagacg cgctctcata agttgctggt
     6421 aatgccatca gaatttacct ccctgcaacg aagtgcgttt ccgtttcgcc cgatgttttc
     6481 gcgagtatgg agagggctaa gcggcggcgt gctctcgcct cgtcgcgcag ttccattgct
     6541 tcgattgtct ctccgcttct acgcttggcg gaagcttccg ccaaaagcag ttcgcagact
     6601 ttcaattgct ctaaggcatg cgcgtgattg aggtgcgccg tgtcgtcgga gcgtcgcata
     6661 tttctggctc ctgttctgtg cggggtgggc gtggtgtcgg gtcgcccggc tagcgttcgc
     6721 tgcttaaagc gttctgagcc gtctggtgtg gacggatgtg acggttcagg agacgcgtaa
     6781 aacatttcgc gcacatgccg cgagaggggc gggcggcggt gcgtcgaata atcgtcccgc
     6841 acgacgtaca tacgaccgcg caacgctgct cgctctgtcc tgattccttg ctcatagcca
     6901 ttaccgatat agcggggacg ccccacctct ggcgggtggt ttgcgtcccc gtttccccct
     6961 tgtcattacc tttcggtatt gttgacgata gtcgataacg ttgaggctgt ctgtacgtag
     7021 ccgcacttag tttcgaccgc gcacagccct cttttcacac gaatttccgg gcgtaaaaag
     7081 ctctgccggg ggatttaaga atagccggac gggcaaggga atttccgtgg cggggagagt
     7141 cttaaactgc cggttcgggc aggagccgcg ccagcaggtt ctttaactgc tcgtgatctt
     7201 cgaggtaggt gatccagtcg ttgttgccga cgttgatgtc ctgaccttcg aggtctgcgc
     7261 cgtacttctc cgccatcacg acgagcggcg tctgcccgtc gtatttcgcg tgctcgcgta
     7321 tcttgcgccc ggtggcgagc acagcttcga gcggtttgcc gacaaggttg atgagcacca
     7381 gatccgcatg cgcgctgtgg ccgtccatcc ttgccagcgc atcctcttca tcgagcgcca
     7441 gcagcacgcg gtagccgtcg acttgaaggt tgtgcttcaa gagcgggcgg gtgtcgtcgt
     7501 cttcttcgat gagaaagata gtgcgccggt tctgctgctc aggcatgctt catttaccct
     7561 ttaattgtat tgaccgatta tgcctttcga ccggaacatt ttcggtacgg taatagacat
     7621 agaagcagat tcccttgatg cgtgccccgg cgtacagagt gcgggagcgt actgtgctgc
     7681 ctgcgtacac ctgctatagt ccgaaccagt taagtcagtc agcctagaac tgcgacataa
     7741 acttagccct gcgatctatc cttatgccga cagcgagtct ctccaaagca tgaggatata
     7801 acttatgacg acgcccgacg gaagcagtct gccaaccact aatcgcattc tcgccgaact
     7861 gccgacagaa gagtacgagc gtctctcgcc tcaccttgaa cccgttacgc tcgcggtcgg
     7921 cgacatgctc tactaccctc aagagcctct cacgcacgtt tatttcccca atcgcgggac
     7981 ggtttccgtg attgcgactc ttgccgacgg cgggggcgtc gaagtgggcg tcgtgggcaa
     8041 tgaaggcttg ttcggtttga acgtcgtcct cggaagtatg agcacgccgc acgaagccat
     8101 ggtgcaactg ccgggcgatg cgtttcggat accggcgcac gtaattatgg gagaatttaa
     8161 gaagggcggg cagcttcagg atttaatcct gcgctacgtg caagccttca tcatccagat
     8221 cgcgcagacg gcggcctgca atcgctcgca tcccatgggc ggcaggctcg cgcgctggct
     8281 cctgatgacc cacgaccgcg ccatgacgga cgagttgctg ctcacgcacg agtttatcgc
     8341 agtgatgctc ggcacgcgcc gggcgggcgt cagcgaagcg gcgggcaggt tgcaaaacga
     8401 agggcttatt aagtacaggc gcgggcatat ccagatcatt gaccgcaaag ggctggaatc
     8461 aacctcctgc gaatgttacc cgctcgtgaa gcgggaattc gaccgcctgc tcggcggcaa
     8521 cggcggcata cactgaaatc agaaccccgc cgcaacctgg acgcacgcgg caactaccgc
     8581 actgtaagga cggcttcgcc ctctttcctt caaattcaaa ctaacgaata aggcgcaaat
     8641 tttccccgtg taccaaagcg tacataaagc gtgttaagct attcgcgtat tacgcgacgg
     8701 ttcttttaag aatccaatat ccgtcatctg gttgagccat gcgcctcctt atgccgtctg
     8761 ctgtcttcct acacacaacc ggactttatt agaagccaaa atcatgtcag aacttcaaaa
     8821 cgcgcgaccg ccgaccgaga accggattct cagagccctc cccgacgaag aatacgaacg
     8881 cctcgcgccc catctggaat tcatcgagac aactcacggc cagattctct accagaccca
     8941 acagacgatt gattacgtct atttcccgaa caaggcaatg atctcgctcg tctcgcaaat
     9001 ggcggacggc ggaagcatag aagtgggcat tgtgggcttt gaagggatgg ccggtctgtc
     9061 cttcgtgctc ggcgttgaca ggtcgccgta cgagacgatg gtgcagattc ataacggcgc
     9121 ggcgcgcatg cccgcccgcc tgctcaagga agagttcaaa cgcggcggca ctttgcagga
     9181 cgtgttgctg cgctacacgc agtcgctcct gctggtgacc tcgcaggtgg ccggttgcaa
     9241 ccgcgcccac tcgctggaag aacgattcgc acgctggctc ctgatgagcc acgaccgctg
     9301 cctctgcgac aacctgccgc tgacgcacga gtttctctcc atgatgctcg gcgtgcgccg
     9361 cgccggagtg acgaccgccg ccctcgccct gcaagccgaa ggattcatcc actacacgcg
     9421 cgggcacata cggatcatca gccgggaagg gctggagcgc atcgcctgcg agtgctacgc
     9481 gacgctcaaa gcggatttcg acatcgtaac ctctggaacg aaaaatgcgg gggggggcaa
     9541 accggaataa atagacacga acacgccaca cacggagagg aaaattccgg cacgctctga
     9601 actccccgca ccgctccgcc cggctctcac acctttcacc ttccgccgcc gccaaactaa
     9661 aacatcagat cgaatccgcc ttcgagcacg cgcgcgagct tgcccgtgcg ccagccgcgc
     9721 acgctgtcgc gcacgagcgg cgaaggttct tcgcggtagc ggcgcacgat ctgcgtctgc
     9781 gcattgttcg ccccgtcggc cagcgcgcgc gcggcctgcc cgcgcacccc ttcacgcccg
     9841 gcaggttcgt ccggttgcgc cacgacgcga acgtgtacgc gcgcacgatt gaaagacttc
     9901 tcatcctgcg gcgtctccag cacgtgccac cccgactcgc cgtgcagcag cgagtaggcc
     9961 gcgaagccgg agactgccgc cagcatttga taaggcgcat ccgccgccga agtagcggtc
    10021 gcatccgccg gagcggcgtc gtccttcgtc tcttctaaaa tctcgaagcg catccgccgc
    10081 gcttcagccc acaggcgata catccctgcg atgcggcgcg caaactcgtt gcagacggat
    10141 gcatccgcgc ccgcgtcgca actggcgcgc acggagagaa aggcatcgcg cgggtgtccg
    10201 gcggcgaggc cgcgcgcggc ggcgtctatg agatagagtt gatgcgcgag gcgcgcgacg
    10261 aggtctgcgg ggaagtgctg gcgtccgcgc gtgtgcgcgc ccgtcaggcg tttgaggagc
    10321 gagcctgccg tgtcgagtcc cgtctcgatg cggtccatgt actccgcgag gccgagcacc
    10381 ccaaagcgcg cgggcgaatt ccagaagccg ggcgcggaag tttccagcag cgcgctcgtc
    10441 ttgcgcccct cccattcttc accttcaacc tgatccgcga gcgcgtcgaa cttgtcttgc
    10501 agaaactgca cctccgcgag cgtgccgcgc gcatccgaga cgatggcttc gagccgcagt
    10561 tcgcgttcgt ccgtgctcgt ctcttcgtcc ggcgcgtcct cttccgcgag cgcacgcgtc
    10621 gtcgccctcg gctcgtcgcc ggaaaattcg agcgcgtccg ggtctataaa ctccacgtcg
    10681 agccgcgcgc cgccgctcgc cgcgcgcacg aagagaaatt gatcgccttc gggaaactgg
    10741 tgattgacaa tcgtgagcgc gagcggcgac aaaaggtaac gctcgatggc gcgtttcaaa
    10801 ggacgcgcgc cgaggtctgc tgtgaaaccc ttctccaaca gaaaatctat cgccgactcg
    10861 tcccactcca ccgcccactg gcgcatccgc aagccgcgac ggcgcagcac gtcgttcaac
    10921 tccttgcgca acagatcgcg catgatgctg cgaccgagcg ggcgaaacac tacgacgcga
    10981 tcaatgcgat tgacgaactc gcgccggaac gcacgcacca ccgcgcgctc gaccgcgccc
    11041 gacgcaaact gcgcctgctc ctgcgaaaat ccgatgctcg cgccgtgcgg cagagtcgcg
    11101 ccgaggttcg aggtcatgat cacgacgcag tggcgaaagt ccgcagtgtt gccgcgcctg
    11161 tccgtgagtc gcccgtcgtc gaagacttgc aggaacaagt cccacacctg cgggtgcgcc
    11221 ttctcgaatt catcgagcag gacgacggag aagggctgtt tgcggatcga gttgacgagc
    11281 gcggtgctct ccgctgtcgt gtcgcgcggc tcgcctaaaa ttcggtctag cgtttcggga
    11341 gtctggaact cgctcatgtc gagccggatc atgcgttcgg gcgaaccgaa gagaaactcc
    11401 gcgagcgtct tggcgatctc ggtcttgccc gtgcctgtgg gaccgacgaa gagaaagacg
    11461 ccttgcgggc gcgtcgggtc ggtcaagccc gccttgatca tcgccacgcg ttccaccagg
    11521 cattcgaccg cctccggctg ccccagcacg cgcccctcga agaagccgcg caactctttc
    11581 aggtcaagcc ccgccctatc gtcgagaatg ctcacgggca gacccgtcaa ctgcgagagc
    11641 gtgacgagca gatcgtcgag cgtcacttcg tttgcagtgc ccctcacgtg ttccgcgccc
    11701 ggctctttgg tcgccactgc cacggttgcg ttgtcgcgtt cgatcaagag gcggcggtgc
    11761 gtggaatcga gcagcaagag cagattgccg ggcgcggcct tgtctgcgag atattgcttg
    11821 gcgagttgga aagcctcctg caaagtctgt tcggagatac gcggcgcggc ggcgttcgtg
    11881 acggcggcgg cgtgcgtctc tgttgcgtcg aagccctgac gcgccgccgt gtcgccgttc
    11941 gcgccgcctg cgtgttgctg catcgcccaa cgacgcgcga cttcgagcgt ctccgcgtcg
    12001 ccgagcgggt tgacgcggca cgtctccaac accgtgcgca ggcgcgggcg caactgcatc
    12061 aacttctcat aagccgccga gggcgtctct ccgacgagct ttaccgcgcc gctctcgatg
    12121 tgcggcagca acaggtcgag caagcccgaa gggttgtatt gatgccgacc cgcccagacg
    12181 agttcgtgaa agttaggcac gacccacagc acgcgcttgc cgccgatctc gcgcacgagc
    12241 gtctgcacgc gcccttcgag ttggccgatg tattgctgcc cggccagcac gtccgccgca
    12301 gaggcttcga gaatgatcca gcgctcctgt tgcaggcgac gcgcgagcac gcgcacgagc
    12361 gcggtcttcc ccgtgcccga atcgccgacg agcaacacag agcgcggcgg ctctttcctg
    12421 agcgcagtct ccatctccag cacgcgctcg cgcacagcct ccagcatcaa cacgccttcc
    12481 ggcgcgtcgc cgttcgcgcc ggacgctttt ccgtcagccg cgccttcaac ctccggcttc
    12541 cacacgcgac cgaacgaccc taggaattcg agatcgacgc gtgcctcttt ccactcgtcg
    12601 aactcgcgca gcaaactctg cggcaacacg tcgcgcaggt cgctgaagac ggtcgtgagt
    12661 agttcgatct gatcgggtga aagcgtgtag agcgcatcgt tgaaggttgc gcccgtctcg
    12721 ccgcaggcga tgcggcgggt gagaaattcg cggagcgatt gcagcggcgt gtattcgccc
    12781 cacgcgcctt tgatgtgcag caggagcggt acgatgacgg ggcgttcgcc cgcgcgtgca
    12841 tcgagcgcgc gcagggcgta gtaacccggc cagtagtaaa actcgttgat gtgcgcgagg
    12901 agcggcgtca cgacatctga cgcgtcgcgc ctttggccga gggcttcgag cgcgatgcag
    12961 gcgacgacga cgtttgtgcc gcgcgccagt tcgatcaggt cgtgcgtgga atatttttca
    13021 tccttcaaca actcgacgcc cttgtgaaaa tattcgtgat gccgcaactc tgcggggcgc
    13081 gaactctgct ggtaatgcgc gtccatcgcc gacgccagcg cgtacacgtc cgtctcgtca
    13141 atcgtgctcg acgcggcgac gacatcggcg gtcgcttgct cgacggaatc cgcgctcgcc
    13201 tcttccgccg ggaaggcttc ggcgagcgaa gccgcgtcgg tcggctggct cgtctcaagc
    13261 tcctgcgacg cgccttcgag cggcacgacg cgttgctcta cagacgggtc tacaggcagg
    13321 gctggcgcaa gcgtcgcagc gtgcgccact ggcaactcct gacgcgcttc ctgctcgcgc
    13381 cgcacgcgtg ccagcgcacg cgcgagatac gcgctgacgc ccacggagag caggcacaac
    13441 aggacgagtc cggcaatgat gagttgcgtc atgggaaatg cgataccact tcaaggacga
    13501 agaattaaag ccgcaaaagt ttaacacccg ttactttgaa agcaacggaa aatactgaaa
    13561 ccatcacccg gcctgcgccg gaagcgtttg acgcctccaa cccgtgccag cggtatttcc
    13621 cacgcgcctt tcagcgaagc gttgaagctg gccttatccc cgctcccgtc gcacgggcgg
    13681 cgctcgttcg tcagcctgcg cggcgtgatc tttcgtcgaa tttttcgaga gtccgccgcg
    13741 cgttaaatta tttctaaatt attcaaattt tcccccgccc gccccttctt cgtgtatgat
    13801 gcacgcgctt tcccatccct tatgacacgt ttagccgacc ccgccggtct gaacccctta
    13861 ggacaaatca gatgctaaga agacacttca tgacactgtt gagctgcgtc tcctgcgcgc
    13921 tcctgttgct cgcttgcggc ggcgcatcca actcgaacaa cgcgcccgcc accaacgcac
    13981 ccgccgccaa caccaacgcc ggtaaggctt ccgcgacgaa caccgccgct ccggcctcta
    14041 ccgctccggc ttccaacgcg ggcagcagcg gcgacacgat tggcgttccc gaatgcgatg
    14101 attacctcgc caagtatgaa acctgcgtct ccagcaaggt gccggaggcg gcgcgtgcgc
    14161 agtatcaatc gacgctcgcg cagacgcgca agtcctggcg cgacctcgcc gccaacccgc
    14221 aaaccaaagc gagcctcgcg caagcctgca agaccgcaac cgaatccgca cgccagacca
    14281 tgaaatccat cggctgcgaa ttttaattct ccgccgcgtc gcgcgaagtc tcccttctca
    14341 ccacaaggac gccggacgct tttcaagcat cgcgcaggta gaagaccctc cgcttccacc
    14401 tgcgcgatgc tttgttcaac ttctcgacac actaaatctt ttctttgcca ctcgcgcctc
    14461 ccccttgagt ttaagtctca ggccggaggc gcaaagtagt tgcgctgtcg cgcgcaaagt
    14521 agttgcgctg tcgcgctcta agagttttcc cgtccatcgg ttataatctc ctttaacttt
    14581 aacgaaacgt ctcatcacat tcgcaataat ttccgaggag caagcatgac cgctgagaag
    14641 atcgccaggc gacccgcgca gatcaagttt gacgggcgca tcctgttttt gaccgacgac
    14701 accacgctca ttcgacgcca gctcgaagcg ggcgaggatt tgcccttcga ccccgaacgc
    14761 ccgctgatga acaacatctc gaccgacgag atcacgcccg gctgggtctg tttctactac
    14821 gacgaaacgc tcgggcagta tgtctacgtc gggatgcgcg aggcggcggt gcagaaggac
    14881 gaagttaaaa acgggggctt caaggtggtc gtctccggcc tctcgaaagg ctgcggctcg
    14941 tcgcgcgaaa ccgcgcccta cgccgagcgc gccgcaggcg tcgaactcgt catcgccaga
    15001 acgattgaga aaatctacgg gcagaactcg cagaacatcg gccttttgac ttcgaccgac
    15061 ttcggcctca tcgaacgcat ccggcgcggc gaagagattc cgctcgcgga gtttacgcgc
    15121 ggcctcgatc cgatctcgca ggcgattgtc gaatacggcg gcctcttcaa ttacaacaag
    15181 gcgcggctcg cgggcgaaac cgtgccaccg cctatcgaaa ccaaaaagcg tccgatgaac
    15241 atcgtcgaga agatcatcgc gcgccacgca tttgtcaggg cgggcgaagt cggcgtcgag
    15301 gccgtgcgtc ccggcgatgc gctgttcgcc gtcgccgacg tgcgcttctc gcacgagtac
    15361 gtgacgccga tggccgagtc tctgttcaaa ggcgcgctcg gacaggacgc gcaggtgacg
    15421 gaagccgaat ccgtctatgc gttccgcgat cacctgacgt ttctggggcg cgtcatgtcg
    15481 gacgacaaga agaagatggg acttctggaa cgcgccgacg ggctggccgt cacgcaggag
    15541 agtttcacgc gcgagcaggg catcaaactt tacagcggca cggaagctac cgggtccgag
    15601 gccatctgcc acaacgccgt cgtcgaagac atcgcgctgc ccggtcaact cgtcatcggc
    15661 acggactcgc acacctgcat ggcgggcgtg ctcggctgct tcgccttcgg cgtcggctcg
    15721 accgacatgg cgaacgcctg gtacacgaaa gacattcgca tcaaagtgcc tgagacggtg
    15781 cgcttcgtgt tgcgcgggca gaagcgcgca gacgtgtcgg ccaaagacgt gatgctcaaa
    15841 attctctcga ccgattacat gaaagaaggg cgtgggatag gtcaggtctt ggagttcgcg
    15901 ggcgaaggac tcgcggagat gtccattgat gagcgcgcga ctttgactaa catggccgtc
    15961 gaagccggag gcacgaccgg catcatcgaa cccgacgaag tgacgctcca atatctcgtc
    16021 aactcgcgcg gactcgacgc cgaggagatt cgccaaggtt tcctcaagag cgacccggac
    16081 gcagagtatg ccgcgacgtt cgagatcaat ctggacgaga tacgcccgat ggtggcgacg
    16141 ccgggcgacc cgcgcaacgg catcaacatc aacgaactca cggaagaagt gaagattgac
    16201 gcggcctacg gcggatcgtg tacgggcggc aagatggccg acatggacat gtacgcggcg
    16261 gtcttgcagc gcgcggtcga gcaggggcgg cgggtcgcgc cgggcgtgcg tctctacctg
    16321 caattcggct cgcagaagat caaacagtat gcgcgcgaac gcggctacat cgaaattttc
    16381 gagcgcgcgg gcgcggagtt gatcgaccct tcgtgcggcg cgtgcatcaa cgctggaccg
    16441 ggcgcgtcga agacggcgga gacggtcacg gtcagcgcgc agaatcgcaa ctttccgggg
    16501 cggagtggtc ccggcaaagt ttatctcgcg tcgccgtatg tggtcgcggc gtcggccatc
    16561 gcggggcgca tcgtcgagcc ggaagagttc ttgcaggagc aagcagagga gttggtcggc
    16621 gcgtgaaagg gatcggcaag ggacacctga gcgcagacca ctcgccgaag tatgagcggc
    16681 tggagcgcga gcggcgatac ctcttgcgcg agttgccgcc cggcctgaaa gtcaacgacc
    16741 agcactcgca gatcacggac aactacatca cgggcacgcg cctgcggctg cgcaaagtgc
    16801 gctggcctct aaccaacgag tggactctga aactgacgca gaagcacgcg cccgcgccgc
    16861 ccgacttctc gcgcacgctc atcacgagca tctacctgaa cgagtacgaa tacgaagtct
    16921 tctccgtctt cgaagcgaac gagctgcgca agaaccgtta tccttacgag cacgaaggac
    16981 gcaaatattc aatcgacgtt ttcctcggcg cgctgcgcgg actcatcctc gcggagacgg
    17041 atttcgacac ggacgcggag atggacacgt ttccgccgcc gcccttcgcc gcgctcgacg
    17101 tgacgcacga cgaactgttc accggcgggc ggctggtcta tctgacgatt gacgaactgc
    17161 gcgcggaatt caaagcgcgc gtcgagaaat gggaggggcg ttgatgagcg gacgttcgag
    17221 cgggttgttc gtcgtagtgg tcggcttcgt gatcgtcggc ttggggttgt tgatgtgggc
    17281 gggcgcgctc tcctggttcg gacgcctgcc gggcgacctg cgcttcggcg gcgaacgcac
    17341 gcgcgtcttc atcccgatca cctcgatgct catcgtctcg ctcctcctga gtctcatcat
    17401 gtgggtggtg cgccgcttct tctgattaac tccgacatgc ccgacaactc ctcgcccgtc
    17461 atctacaccg tcggacacgg acgccacgcg tgggctgact ttctcgcgct gctccgcgag
    17521 cacgagatcg cgctcgtcgt ggacgtgagg agcgcggcgc gttcgcgttg gccgcagttc
    17581 aacgggcgcg tgttggaggc gaatttgcgg gaggcgcgga tcggttacga atggttgccg
    17641 gagtgcgggg ggaaggtggt cgcgccgccg ccggaactcg cgcgggggct ggaacgaatc
    17701 atggagctgg cgtcggaagt gcgcgtcgct ttgatgtgtt cggaatcgca gccgctcacg
    17761 cgccacacgc agccgcgcgc caactgtcac cgcgtcggcc tgctctcgac gccgcttcgc
    17821 gcacgcggcg cacgcctcat acacatcctg cccgacggcg acacgctcga aatcgacgag
    17881 gccgccctgc cctccatctg gaaggaagag acgctgttcg gcgacagtta aattaatccg
    17941 attacagatt ggcatccggc agccttgcgc cgcttttaaa agtgactggt gggaagcaag
    18001 ccgcctgagc taccggacgg ctctttattc gtctctgttc tcgtcaacct tcggcgggtg
    18061 tcggaaattc cgtgtgggcg taggcgtcgg cgtgggcgcg tgcgaagtag gtggcgagca
    18121 ggaaggcgag gagcatgatg gcggcggcag tccagaaggt gacgagcagc gagcggtcgc
    18181 tcatctcgtg gagccgcccg tctgcgccgt aggtggcgac cgcgctgttg atgaggagcg
    18241 cggcgatcat cgggccgacg gcgcgcgcca gactcgccac cgattgcacc acgccgagca
    18301 cgccgccctg ttcgccccgc ccaacgcttc tggaggcgag cgtttgcagc gaaggcgtcg
    18361 ccagcgaatt gccgagcgca aacgttccgc cgaccagcag cagcagaggc agccccgcgc
    18421 cctgaccgag cagcggaatc gcaaacagac tcagtgtaaa gagcagcgcg ccgacgatga
    18481 ccagaaacat ctcgccgaac attttgacga gacgcccgat gagtcctccc tgaatgatcg
    18541 cgccgatgat gccgacgtac atgaagagat aaccgttatg tgaagcgtcg tagccgtagc
    18601 gatacatcgt gaagagggca aacga
//
LOCUS       DASWNS010000469         7689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_406731, whole genome shotgun sequence.
ACCESSION   DASWNS010000469 DASWNS010000000
VERSION     DASWNS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406731"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VGB76_16160"
     CDS             <1..755
                     /locus_tag="VGB76_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY0100480.1"
                     /translation="NLSGLTGASFDVRDDNTEGIRVSNGQLETTTRGGVKTTVQGNEY
                     IALNQQGSIKSRESLLAMPLLVSPRDLEKISATDKGATSVTLRWVRPPTGAPRHYRVE
                     VATSPFFVQAGKVTERDQLEATQFSVSDLRAGIYFWRVRAVAASGQISEWTEPQKFTI
                     VAGQGTGDHISISGVAFEFIGGSIYVVRGRTQPGNTIRISGRETLAAADGSFQLQISV
                     PRAAREIIVEAEDSQGTRKDTRLAFTPNAGQS"
     gene            837..2339
                     /locus_tag="VGB76_16165"
     CDS             837..2339
                     /locus_tag="VGB76_16165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY0100481.1"
                     /translation="MQVILLKVEHERPGRALLEALREAGVCATEMAAVGSSRMGEGVV
                     EVSPLALICEIAEDVSIAEVHAAITEAAAAWPEASVVACRRFEAGEPQPELYRLDTHA
                     LLRLGFHAVADDPAQLPALLRDLEEAGKNLPESATHSEVLDTTATPASMLLPDNLSVA
                     RLRAAFEAVASLHFVSDQKSAAQTALAGLAPLVTADRWTIYLAELERGQQVARFEPLA
                     VRGLTESERALPEGDWRRALHTDSLMLLTGTESQAARNAFATNETLRETEGARRVLAV
                     PLVNGERVVGILEAVREGEAAPGFASSEAALISALALPLSAALANSVRIAEAEKLSQT
                     DDLTKLHNARYLRQFLIAEIKRARRYGSTVTALFFDLDDFKEVNDRYGHLVGSHVLME
                     MATVILTGVRDTDVVARYGGDEFVVILPETNLDMASRVAERMREKIAEHVFNGGRSLR
                     LRMTASFGVAAFPEHSQSPQQLIADADAAMYEAKAAHKNCVRFASKPEPR"
     gene            2620..3666
                     /locus_tag="VGB76_16170"
     CDS             2620..3666
                     /locus_tag="VGB76_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY0100482.1"
                     /translation="MAFKSLFSLFSSDLAIDLGTANTLVYAKGRGIVVSEPSIVAINK
                     STNQPLAVGKEAKEMLGRTPGNIVAIRPMKDGVIANFEVTEKMLQHFIRKAHNGKSWV
                     SPRVVIGIPSEITQVERRAVEDSAYRAKASEVYLVEEAMAAAIGAGLPITEPHGNMIV
                     DIGGGTTDIAVISLSGIVYSRAVRVAGNEMDEAITGFIKRKYNLLIGERTAEMIKIQL
                     GSAFPLDEQLSMDVRGRNLIEGIPKTITITDDEVREALADSISTIVNAVRVALERTPP
                     ELSADIVERGIVLTGGGALLKNLDRRLSIETGLPVSVAEDPLSSVVLGAGKMLSDFNL
                     LKRVKWDNNLTSGT"
     gene            3752..4642
                     /gene="mreC"
                     /locus_tag="VGB76_16175"
     CDS             3752..4642
                     /gene="mreC"
                     /locus_tag="VGB76_16175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY0100483.1"
                     /translation="MAVTRTQKEIRQRAPLWLLALLAVNLVLMSVSARDESGHKRLIQ
                     VWAQGLISPVQSLFSGATGAGTGFFQHLGSLNRAATENEELRRRLATTETELREARMA
                     RDENERLKGLLELKERGEYQTVTARVIARDPSEWFDSVMINRGSSSGIERDMPVVTHE
                     GIVGRIVGVSPYTAQVLLVTDERSAAGAVVGQLGASQAMGSVRGYGKNGLLDMRYVSG
                     LEAVKQGDMVVTTGQDSIYPPGLSVGEVVEFKQGSATSPHEIKVKPTARLNSLGEVAV
                     LLYRAPPRTPPADAIKGSAK"
     gene            4743..5273
                     /gene="mreD"
                     /locus_tag="VGB76_16180"
     CDS             4743..5273
                     /gene="mreD"
                     /locus_tag="VGB76_16180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY0100484.1"
                     /translation="MKLKIALCVALAVLLQTNLRLIWPPLVYFDLPLIVVTYFALRRD
                     VVQALFVGFVAGLGTDVFSRGLLGANGFSKTLIAYLIAALVTRIMLDNPLARIPVLAG
                     AAAFDAVVVVFLHRLLGQPSLVPFAETTAYKVIATTIAGTFIFYMFDIFFSDRARQRR
                     HFAFRRRAARRSLVRR"
     gene            5437..7401
                     /gene="mrdA"
                     /locus_tag="VGB76_16185"
     CDS             5437..7401
                     /gene="mrdA"
                     /locus_tag="VGB76_16185"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY0100485.1"
                     /translation="MKFHDESQNLRARLRIIQVLVFVLLAILGVRLYFLQVVNGAYYA
                     ERAENQRVRLLPIPAQRGTIFDRNGRILVDSRPIYNVILSREELHGKDLNSLVAPLSA
                     ALALDPELLRERFAELKSQPAFESIIIKQNASMADIQWVETRQAFDHPELRIEQQPQR
                     RYPENGILAHVLGYVAEVNPKQLEREEYKVKGLKPGDIIGQEGIEATYDDYLRGRDGY
                     RKVIVDSRGRIQDEIEKVPPQPGQDIVTTIDLEMQEMAEEQLRNSPSGRGVIISVDPN
                     NGEILVMASAPTFDPNLFSQRISTKGGRAEYAALIKDPRRPLYNRAIRGRYPPGSTWK
                     IPMAVAGLEQGQITTEHSNLVCGGGITVGNKFTRCMGHHGSPELRTAIKHSCDGYFYR
                     LGLKMKLEGIMQMVESFDLNKRTGVDLPHELISWTPSREFKKKFQPKAAEWGDIDTVF
                     ASFGQVYENMTPLSMLRTIAGISVGGKLFVPHLLKEVRAIPAMGEPGRFDYRPAREAR
                     TFVPLDTSRPNPKVVPISEEHHRMVVEGMWQVVNQPGGTGGAMQMAGFDVAGKTGTAQ
                     VVSLGKDVGENKDHSWFVAYAPAHKPEIASIALIENVGFGGKFAAPASKAVYEVYYRK
                     TRPDAAPPPQVAQQNRGQKPEVRGQANPID"
     gene            7441..>7689
                     /locus_tag="VGB76_16190"
     CDS             7441..>7689
                     /locus_tag="VGB76_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100486.1"
                     /translation="MVAILEKGRFRDFDWLLTLLATGIVFFGTWQIHNAQPLENYWKK
                     QLIGLGISLVALFAISFTDYRKLVHFAPAFYAFGLFLLI"
BASE COUNT         1529 a         2443 c         2268 g         1449 t
ORIGIN      
        1 acaacctctc agggctgacc ggcgcgagtt tcgacgtgcg cgacgacaac acggaaggca
       61 tccgcgtcag caacggccaa ctcgaaacca cgacgcgcgg cggcgtgaaa accacagtgc
      121 agggcaacga gtacatcgcg ctcaatcaac agggcagcat caagagccgc gagagcctgc
      181 tggcgatgcc cctgctcgtc tccccgcgcg acctcgaaaa aatatcggcc acagacaagg
      241 gcgcaaccag cgtcacgctg cgctgggtgc ggccaccgac gggcgcgccg cgacattacc
      301 gcgtcgaagt cgccacgtcg ccgttcttcg tacaggccgg gaaggtaacc gagcgcgacc
      361 aactggaggc gacgcagttc agcgtcagcg acctgcgcgc gggcatctat ttctggcgcg
      421 tgcgcgccgt ggccgcttcc ggccagatca gcgaatggac tgagccgcag aagttcacca
      481 tcgtcgcggg gcaaggcacg ggagatcaca tctcgatttc gggcgtcgcg ttcgaattta
      541 tcggcggcag catctacgtg gtgcgcgggc gcacgcaacc gggcaacact atccgcatca
      601 gcgggcgcga aacgctggcc gccgccgatg gctctttcca actccaaatc tccgtgccca
      661 gagccgcacg cgaaatcatc gtcgaagccg aagactcgca aggcacacgc aaagacacgc
      721 gccttgcctt cacccccaat gcgggacaga gctaaaagtg atgagtgatg agtgatgagt
      781 gatgagttaa acctcttctc actcatcact catcactcat cactcatcac ttagttatgc
      841 aggtcatcct gttaaaagtt gagcatgagc gtccggggcg cgctttgttg gaggcgttgc
      901 gcgaggcggg cgtgtgcgcg acggagatgg cggctgtcgg gtcgtcgcgg atgggcgagg
      961 gcgttgtgga agtgtcgccg ctcgcgttga tctgcgagat cgcggaggac gttagcatcg
     1021 ccgaggtgca tgcggcgatc acggaggccg ccgccgcgtg gcctgaagcc tctgtcgtcg
     1081 cctgccgccg ttttgaagcg ggcgagccgc aacccgaact ctaccgcctc gacacgcatg
     1141 cgctgctccg tctcggcttc cacgccgtcg cagacgatcc ggcgcagttg ccagccctgc
     1201 tgcgcgacct cgaagaggcg ggcaagaatc tgccggagtc ggccacgcac tccgaagtcc
     1261 tggacacgac ggcgactccg gccagcatgt tgctgccgga caatttgagc gtcgcgcgcc
     1321 tgcgtgcggc ctttgaagcg gtcgcctcgc tccatttcgt gagcgatcaa aagagcgcgg
     1381 cgcagaccgc cctcgccgga ctcgccccgc tcgtcaccgc agaccgttgg acgatctatc
     1441 tcgcggagct tgagcgcggc caacaggtcg cgcgcttcga accgctcgcc gtgcgcgggc
     1501 tgaccgagag cgaacgcgcc ctgcccgaag gcgactggcg acgcgcgctc cacaccgact
     1561 cgctgatgct cttgaccggc acggaatcgc aggccgcgcg caacgcgttt gccaccaacg
     1621 agacgttgag agagacggaa ggcgcgcggc gcgtcttggc cgttccgctc gtcaacggcg
     1681 agcgcgtcgt gggaatactg gaagcggtgc gcgagggcga ggcggcaccc ggtttcgcgt
     1741 cgagcgaagc ggcgctcatc tccgcgctcg cgctgccgct ctcagccgcg ctcgccaact
     1801 ccgtccgcat cgccgaagcg gagaaacttt cgcagaccga cgatctcacc aagctgcaca
     1861 acgcgcgata cctccgccaa ttcctgatcg ccgagatcaa acgtgcccgg cgctacggct
     1921 caaccgtgac cgcactcttc ttcgacctcg acgacttcaa agaggtcaac gaccgctacg
     1981 gccatctcgt cggctcgcac gtcttgatgg agatggcgac cgtgatcctg acgggcgtgc
     2041 gcgacacgga cgtggtggcg cgctacggcg gcgacgaatt cgtcgtcatc ttgccggaga
     2101 cgaatctgga catggcctcg cgcgtcgccg aacgcatgcg cgagaagatc gccgaacacg
     2161 tcttcaacgg cgggcgcagt ctgcgcctgc gcatgacggc cagtttcggc gtcgccgcct
     2221 tccccgaaca ttcacagtcc ccgcagcagt tgatcgcaga cgccgacgcg gccatgtacg
     2281 aagccaaagc cgcgcacaaa aactgcgtcc gcttcgcctc caagcccgag ccgagataac
     2341 tcaagcccga cttctctccc cggcgtttag cgacgcgccc cgcccgaatc cgaatttccg
     2401 cattaaaata gccgacttga gcctgttggc tttagtattt gggctttttc gttgtatctt
     2461 gttgagttgg tagggcgtcg cgtggcgcga gcctcgcagg gtgctgtagg aagtcggact
     2521 tacgtgctgt tatttcatgt aatcctctaa cttcgctcgc cccgcgctct gtaaccttca
     2581 tttctgacgc aacacattca tgcttggata gaaaaattaa tggcctttaa atcactcttt
     2641 agtctatttt ccagcgatct ggcgatagac ctcggaactg ccaacacgct cgtctatgcc
     2701 aaaggacgcg gcatcgtcgt gtcggagccg tctatcgtcg ccatcaacaa gtccaccaac
     2761 cagccgctcg ccgtcggcaa ggaagccaaa gagatgctgg ggcgcacgcc cggcaacatc
     2821 gtcgccatcc gcccgatgaa ggacggcgtc atcgccaact ttgaggtgac ggagaagatg
     2881 ctccaacact tcatacgcaa ggcgcacaac ggcaaatcgt gggtcagccc gcgcgtcgtc
     2941 atcggcatcc cttccgagat cacgcaggtc gaacgccgcg ccgtcgaaga ctcggcctat
     3001 cgcgcgaagg cttcggaagt ctaccttgtc gaagaagcga tggcggcggc catcggcgcg
     3061 gggctgccca tcaccgaacc gcatggcaac atgatcgtgg acatcggcgg cggcaccacc
     3121 gacatcgccg tcatctcgct ctcagggatc gtctattcgc gcgccgtgcg tgtcgccgga
     3181 aacgagatgg acgaagcgat cacgggtttc atcaaacgca agtacaacct gctcatcggc
     3241 gaacgcaccg ccgagatgat caagattcaa ctcggctcgg ccttcccgct cgacgaacaa
     3301 ctctcgatgg acgtgcgcgg gcgcaacctc atcgaaggca tccccaaaac catcaccatc
     3361 accgacgacg aagtgcgcga agctctggcc gattcgatct cgaccatcgt taacgccgtg
     3421 cgcgtcgcgc tcgaacgcac gcccccggaa ctctccgcag acatcgtgga gcgcggcatt
     3481 gtcttgacgg gcggcggcgc gctcttgaag aaccttgatc gtcgcctctc catcgagacg
     3541 ggcttgcccg tctccgtcgc cgaagacccg ctctcgtccg tcgtgctcgg cgcgggcaag
     3601 atgctctcgg acttcaacct tctcaagcgc gtgaagtggg acaacaacct gacgagcgga
     3661 acttaaaagc cgtgaatcgt aaatcgtaga tcgtgaatag agaaacccgg cctgagtcta
     3721 tttacgattt acgattcact atttactaac tatggctgtt acgcgaacgc agaaagagat
     3781 caggcagcgc gcgccgctgt ggttgctcgc gctgctggct gtgaatctgg tgttgatgtc
     3841 cgtctcggcg cgcgatgaat cgggacacaa gcgtctgatt caagtctggg cgcaagggtt
     3901 gatctcgccc gtgcagagcc tcttctccgg cgcgaccgga gccgggacgg gcttcttcca
     3961 acacctcggc agcctcaacc gcgccgccac cgaaaacgaa gaactcagac gcaggctcgc
     4021 caccaccgaa accgaactgc gcgaagcgcg gatggcgcgc gacgagaacg aacgcctcaa
     4081 gggactcctc gaactcaaag agcgtggcga ataccagacc gtcaccgcac gcgtcatcgc
     4141 ccgcgacccg tccgaatggt tcgactcggt gatgatcaat cgcggcagtt catcaggcat
     4201 cgaacgcgac atgccggtcg tgacgcatga ggggatcgtc ggtcgcatcg tcggcgtcag
     4261 cccgtacacg gcgcaggtgt tgctggtcac ggacgaacgc tctgcggcgg gcgcggtcgt
     4321 cggccaactc ggcgcgtcgc aagcgatggg ttcggtgcgc ggctacggca aaaacgggct
     4381 gctcgacatg cgctacgttt cagggctgga ggcggtcaag cagggcgaca tggtcgtgac
     4441 gacggggcag gacagcatct acccgccggg cttgagcgtc ggcgaagtgg tggaattcaa
     4501 gcaggggtcg gccacctctc cgcacgagat caaagtgaaa ccgaccgcgc gtttgaactc
     4561 gctcggagag gtcgccgtgc tgctctatcg cgcgccgccg cgcacgccgc ccgccgacgc
     4621 tattaaaggc agtgccaagt gacaaggtga cgagtgacaa ggtaaatcag aaacctcgtc
     4681 tttaaacttg tcttttcatc tcgtcacttg tcacctgtca ctcgtcactg ttttttgttt
     4741 ttatgaagct gaagatcgcc ctgtgtgtcg cgctggccgt gttgttgcag acgaatttgc
     4801 gtctcatctg gccgccgctc gtctatttcg atttaccgct catcgtcgtc acctatttcg
     4861 cgctgcggcg cgacgtggtg caggctctct tcgttggctt cgtggccgga ctcggcacag
     4921 acgttttcag ccgtggactt ctcggcgcaa acggtttctc caaaacgctc atcgcttatc
     4981 tgatcgccgc gctcgtcacg cgcatcatgc tcgacaaccc gctggcgcga attcccgtct
     5041 tagcaggtgc ggcagccttc gacgcagttg tcgtcgtctt tctccatcgc ctgctcggac
     5101 agccctcgct cgtgcccttc gccgagacga cggcctacaa ggtgatcgca acgaccatcg
     5161 cgggcacgtt catcttttac atgttcgata tttttttcag cgaccgcgcg cgccaacgcc
     5221 gccatttcgc ttttcgtcgc cgcgcggcgc gtcggagtct ggtcagaagg tgaaagaaca
     5281 gtgacgagtg acaggtgacg agtgacgagc aacgagcgat gagttagaat ccatgttgag
     5341 ggagtcgccg gctcgtctct taaacttatc ccccttcact tgtcacccgt caacttgtcg
     5401 accgttcttt aacttgtcac acgtcactgt atttttatga aatttcacga cgaatcgcaa
     5461 aatctgcgcg cgcgtttgcg gatcattcag gtgctcgtct tcgtgctgct ggcgatcctt
     5521 ggcgtgaggc tctacttttt gcaggtggtc aacggcgcgt actacgccga gcgcgcggag
     5581 aatcagcgcg tgcgactgct gcccataccg gcgcagcgcg gcaccatctt cgaccgcaac
     5641 ggtcgcatcc tcgttgactc gcgcccgatc tacaacgtca tcctctcgcg cgaggagttg
     5701 cacggcaaag acttaaactc gctcgtcgcc ccgctctcag ccgcgctcgc gcttgacccc
     5761 gaacttttgc gcgaacgttt cgccgaactc aaatcgcagc ccgccttcga gtccatcatc
     5821 atcaagcaga acgcttcgat ggccgacatc cagtgggttg agacgcggca ggctttcgac
     5881 catcccgaac tgcgcatcga gcaacaaccg cagcgtcgct atccggaaaa cggcatactc
     5941 gcgcacgtcc tcggctacgt cgcggaagtc aatccgaaac agttggagcg cgaagagtac
     6001 aaggtgaagg gactcaagcc cggcgacatc atcgggcagg aaggcataga agccacctac
     6061 gacgattatt tgcgcgggcg cgacggctac cgcaaagtga tcgtggatag tcgcgggcgg
     6121 attcaggacg agatcgagaa agtgccgccg caacccggtc aggacatcgt caccacgatt
     6181 gatcttgaga tgcaggagat ggccgaggaa caactgcgca actcgccttc cgggcgcggc
     6241 gtgatcatct cggtcgaccc gaacaacggc gagattctgg tgatggcctc cgcgcccacc
     6301 ttcgacccca acctcttctc gcagcgcatc tccaccaaag gcgggcgcgc cgaatatgcc
     6361 gcgctcatca aagaccccag gcgaccgctc tacaatcgcg ccatacgcgg gcgttacccg
     6421 cccggttcga cgtggaagat tccgatggcc gtggcgggct tggagcaggg acagatcacg
     6481 accgagcact cgaatctcgt gtgcggcggc ggcatcacag tcggcaacaa gttcacgcgc
     6541 tgcatggggc atcacggctc gcccgagttg cgcacggcga tcaagcattc ctgcgacggc
     6601 tatttttacc gtctcggttt gaagatgaaa ctcgaaggca tcatgcagat ggtggagagt
     6661 tttgatctca acaaacgcac gggcgtcgat ctgccgcacg aactcatcag ttggacgcct
     6721 tcgcgcgagt tcaagaagaa gtttcagccg aaggccgccg aatggggcga catcgacacg
     6781 gtcttcgcct ccttcgggca ggtttacgaa aacatgacgc cgctctcgat gctgcgcacg
     6841 attgcgggca tcagcgtcgg cggcaaactc ttcgtgccgc acctgctcaa agaggtgcgt
     6901 gcgattccgg cgatgggcga accgggcagg ttcgattacc gtccggcgcg cgaggcgcga
     6961 acctttgtgc cgctcgatac gagccgtccg aacccgaaag tcgtgcccat ctcggaggag
     7021 catcaccgga tggtcgtcga aggcatgtgg caggtggtga atcaaccggg cggcacgggc
     7081 ggtgcgatgc agatggccgg gttcgacgtg gcgggcaaga ccggcacggc gcaggtcgtc
     7141 tcactcggca aggacgtggg cgagaacaaa gaccactcct ggttcgtcgc ctatgcgccc
     7201 gcacacaagc ctgagatcgc ctccatcgcg cttatcgaaa acgtcggctt cggcggcaag
     7261 ttcgccgccc cggcctcgaa ggccgtctac gaggtttact atcgcaagac gcgccccgat
     7321 gccgcgccgc cgccgcaagt cgcgcaacag aacagaggtc agaagccaga ggtcagaggt
     7381 caggcaaatc cgatagacta acttgtcgcc tgtcactctt cacttgtcac tgttttaatt
     7441 atggtcgcaa ttttagaaaa aggccgcttc cgtgatttcg actggctgct gacgctgctg
     7501 gcgaccggga tcgtattctt cggcacgtgg cagattcaca acgcgcagcc gcttgagaac
     7561 tactggaaga agcagttgat cgggttgggc atttcgctcg tcgcgctctt cgccatctcc
     7621 tttacggact atcgcaagct cgtccatttc gcgcccgcct tctacgcttt cggcctcttc
     7681 ctgctcatc
//
LOCUS       DASWNS010000470         7011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_458088, whole genome shotgun sequence.
ACCESSION   DASWNS010000470 DASWNS010000000
VERSION     DASWNS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458088"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VGB76_16195"
     CDS             <1..204
                     /locus_tag="VGB76_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEY0100487.1"
                     /translation="RYKGLGEMNPEQLWETTMNPEKRTLLQVKIDDAVETDSIFTVLM
                     GDAVEPRRKFIEDNALDVKNLDI"
     gene            complement(567..779)
                     /locus_tag="VGB76_16200"
     CDS             complement(567..779)
                     /locus_tag="VGB76_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100488.1"
                     /translation="MADNDKTKTPSGEAAAPDMTSDTTTEDRRTLDIGESGQFAPGGY
                     YNQQGATKTDRLDLDEQFSDATDDKS"
     gene            complement(819..1433)
                     /gene="amrA"
                     /locus_tag="VGB76_16205"
     CDS             complement(819..1433)
                     /gene="amrA"
                     /locus_tag="VGB76_16205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEY0100489.1"
                     /translation="MKTETDMGSRQRENSNPTEAESSPHADPAPHDEGRLPELARRTV
                     ETFVREGRVPETPPVADDSLLGLRAACFVSIKTDEGDLRGCIGTVEPTKATLAEELIH
                     NAISAATRDPRFLPVSAPELPYLRYSVDVLSPPEPTRFEELNPKTYGLIVEDGRGRRG
                     LLLPDLEGVETPEQQLQITARKAGIASGAGDVKLYRFRVRRYGE"
     gene            complement(1441..2253)
                     /locus_tag="VGB76_16210"
     CDS             complement(1441..2253)
                     /locus_tag="VGB76_16210"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HEY0100490.1"
                     /translation="MAGSIVFVGIAPHPPIMVPEVGREAIAEVRGSIEAMRELTERII
                     ASGAETIIIISPHAPLEARAFVAYSETELRGDFANFRAPQATINAPLDLELLEAITSS
                     AATDGYKIVPLEGYELDHGTAVPLYFFLRNGWRGRVVALGYSFLSNEDHVRFGACIRR
                     AVEASGRAAVAFVASGDLSHRLTPDAPAGFNPQAHLFDEQVLASINAQTPDRIAGIDQ
                     NLRRLAGECGYRSMLVAIGTEDESARDCEVLHYEAPFGVGYLVAQLARAAAR"
     gene            2517..3749
                     /locus_tag="VGB76_16215"
     CDS             2517..3749
                     /locus_tag="VGB76_16215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0100491.1"
                     /translation="MIESGEFDLLIVGAGPAGSFAAERLARGGARVALFDGRPVGEAK
                     ACGGGVTSKALKAWPHLLEALGRTVEEVEMYAPAGTRVRLKLDAPFAIYSRAAFDSYL
                     RDRAIQAGAHVISERVSVAMDNEKKELWTARGRSGARWQGRVLVAADGANSPIARRLA
                     GALPNAEMEVAFGYRAPLPKNGEAPTVIAFLPNWVGYAWAFPRLDHISFGIATSQDAF
                     KHRALDALLWEFMLGYYRMRDDASAPLWSSQAKPTPEDELRDREIERKLRASVNRYAA
                     RIPGLAPETWNTRRVGSQTWALLGDAAGFADPVTGEGIYYALRSAEIFAEAFLEGDTA
                     AYEERWRADFGRELRRASAMRRRFYGEFLGAPFTDRMIDFARLHPGIRRTLRELVAGD
                     QGYVNLKRALARSALWPL"
     gene            complement(3890..4348)
                     /locus_tag="VGB76_16220"
     CDS             complement(3890..4348)
                     /locus_tag="VGB76_16220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0100492.1"
                     /translation="MSQQKSETEAWKFRIEERVRWGDVDAARIIFYGAYIRFFEIAET
                     ELFRSVGLPYGRMFDELDVWLPRAHLECDFRQAAQLDDLLEVSVYVGRVGTKSLRLNF
                     EVRRNGDPSIVAEAHFVLVAVRRNTFDSVPVPDELKRRLAPYTQPVPGEP"
     gene            complement(4416..5219)
                     /locus_tag="VGB76_16225"
     CDS             complement(4416..5219)
                     /locus_tag="VGB76_16225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0100493.1"
                     /translation="MSVVHVRGIELAYEASGAGLPVVLLHGFPFNRTLWREQVEALRE
                     RYTVVTVDLRGHGETHATHDDSPATMEVMAEDVAALLDELRLARVVLGGLSMGGYVAL
                     AFYRLFPERVRALVLADTRPQADTDEARLGREETAARALSQGMQSIADAMLPKLFAPA
                     TQTERPEIVARVRDMILQTDPRGAAAALHGMALRADQRELLREIDCPVLVVVGRLDAV
                     TPLADSASMSREIPGARLEVIEEAGHVSNLERPAGFNRALVDFLDALEP"
     gene            5504..5866
                     /locus_tag="VGB76_16230"
     CDS             5504..5866
                     /locus_tag="VGB76_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100494.1"
                     /translation="MSSKKQTTKAQIFSAAEQRAARSERIEVREAEPYGWIAFSESGT
                     EHNYHLFRDPETKRLACTCADFIYRGDAEPRYECKHVSATLKYIARQYLERDYDPQQQ
                     YARTSAALESHHSTRRAA"
     gene            complement(5879..>7011)
                     /locus_tag="VGB76_16235"
     CDS             complement(5879..>7011)
                     /locus_tag="VGB76_16235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0100495.1"
                     /translation="LIVKILQLSSARTFGGGERHFADLSNALSERGQEVYAALSPASP
                     LREKLTRIPGANISTLPLRNALDLASASQLARFIHDHEIELVHAHLARDYPLAAYAAR
                     RAGERVPFVLTRHVPFALNRLHRFTLANVARVIAVSEGVARGLRQQRIFPGEKIRVIP
                     NGISVAGLDTALKDFDRARFRQTLQPASAALIVGTIGELSPVKGQEEFVRAAASIIRR
                     ENFSRAQFLIIGEDRSPRAPTRTLLEKLIAEHELQGHVRLLGYVSELTPLINSLDLYV
                     SASRAEAFGLATLEALMCGIATVATGTDGARELIEDGVTGKLVPIGDADALARAMLEL
                     LRNDDERTRISERARESARARFSLEQMVEATEKVYREVLAAG"
BASE COUNT         1338 a         2056 c         2229 g         1388 t
ORIGIN      
        1 cgctacaagg gtctgggcga gatgaacccc gaacagcttt gggaaactac gatgaaccct
       61 gagaagcgca cgctcttgca ggtcaaaatt gacgatgcgg tggaaaccga ttcgatcttc
      121 acggtcttga tgggcgacgc cgtcgaaccg cgccgcaagt tcatcgaaga taacgctctg
      181 gatgtgaaaa atctggatat ttgatcattt agacagagcg gcgggcaggc acgtcgagtt
      241 gtaaatttga cacttctcac ccgctcgctt aaaatgccat cctttcctcc tgtggatcat
      301 tgcccgctgc atgcaactcg cgcatgcagc ggcttttttg tgtgcggtag tttttcctga
      361 taccgcgaaa aaaaggcggt taacagtgga ttgcgattga gtggggcgaa tgtgagggaa
      421 gtccgtgctg ctgcaacgcg gaagggcgca gagacagttg atcgttcaat ctctctctgc
      481 gcccgttgcg gttttactct gcgccgccct ccctgcgttg aagttttaaa acgactggcc
      541 gcgtaaaagg taaaatccgt gcaatcttag ctcttatcgt cagttgcgtc gctgaactgc
      601 tcgtccaaat cgaggcggtc ggttttggtc gcgccttgct ggttgtagta accgccgggc
      661 gcgaattgtc ctgattcgcc gatgtcgagg gtacggcggt cttcggtggt ggtatccgat
      721 gtcatgtccg gggcagcggc ctcgcccgaa ggtgtcttgg ttttatcatt atcagccatg
      781 tcaatttctc ctcgtgtttg aattttagaa aagactctct attcaccgta gcggcgaact
      841 cggaagcgat acaatttcac gtctcctgcg ccggacgcga tcccggcttt gcgggcggtg
      901 atctgcaact gttgttcggg cgtctcgacg ccttcgagat cgggcaggag cagtccgcgc
      961 cgtccgcgtc cgtcttcgac gatgaggccg taagttttgg gattcagttc ttcgaaacgg
     1021 gtcggctccg gcggcgaaag cacgtcgacc gaataacgca ggtacggcaa ttcgggcgcg
     1081 gagactggta aaaaacgcgg gtcgcgcgtc gccgcactga tcgcgttgtg gattagttct
     1141 tcggcaagcg tggctttggt gggttcgacc gtgccgatgc aaccgcgcag gtcgccctcg
     1201 tcggttttga ttgagacgaa acaggccgcg cgcaagccga gcaacgaatc atccgcgacg
     1261 ggcggagttt ccggcacgcg cccctcgcgc acaaaagtct ctacggtgcg gcgcgcgagt
     1321 tcgggcaggc gtccctcgtc gtgcggcgcg gggtctgcgt gcggcgacga ctcggcctct
     1381 gtcgggtttg agttttcccg ctggcggctg cccatgtcag tctcggtctt cataaactgt
     1441 tcaacgcgcg gctgcccgcg cgagttgagc taccagatag ccgacgccga acggcgcttc
     1501 gtaatgcaaa acttcgcaat cgcgcgccga ctcgtcctcc gtcccgatgg cgacgagcat
     1561 cgagcgatag ccacattcac ctgcgaggcg tcgcagattt tgatcaatgc cagcgatccg
     1621 gtcaggcgtc tgcgcgttga tggatgcgag cacctgttca tcgaagagat gcgcttgagg
     1681 gttgaagccc gcgggcgcgt cgggcgtcag gcgatggctc aaatcgccgc tcgccacaaa
     1741 cgcgaccgcc gctcgcccgc tcgcttcaac cgcgcgccgg atacacgcgc cgaaacgcac
     1801 atgatcttcg tttgacagaa aactataacc gagcgcgacg acgcgaccgc gccatccgtt
     1861 gcgtaaaaag aaatagagcg gcacggcggt tccgtgatcg agttcgtagc cttcgagcgg
     1921 gacgatcttg tagccgtccg tggcggcgga tgaagtgatc gcttcgagca gttcgaggtc
     1981 gagcggggca ttgatggtcg cttgcggagc gcgaaagttg gcaaagtcgc cgcgcagttc
     2041 cgtctcactg taggccacga aagcgcgcgc ttcaagcggc gcatgcggcg agatgatgat
     2101 gatcgtttcc gcgccggacg cgatgatgcg ttcggtcagt tcacgcatcg cctcgattga
     2161 gccgcgcact tcggcaatag cttcccgccc gacttcagga accatgatcg gcgggtgcgg
     2221 tgcgatgccg acgaaaacaa tggagccagc catattcaga gccttaacgc gtgtgtcatg
     2281 tgcagccgta ttgcaacgcc tgctcgtctg atccaactgc ctgcgggggc aaagagaaac
     2341 aactcgggcg accagatcgg ggactgaccg gcaaaacatt ttgcgggatg gcgaaattct
     2401 atcacaaccc gagcgcgcca tgacaagaag agcgtgcgcg cccgtgataa catctcccgc
     2461 ctcgacgtta ggatgggcgg cgcgatttct ttgcgagcgg gaaagcgaaa gagcgaatga
     2521 ttgagagcgg ggagtttgat ctactgatcg tcggcgcagg accggcgggg tcgtttgcgg
     2581 cggagcgtct ggcgcgtggc ggtgcgcgtg tcgcgttgtt tgacgggcgg cctgtcggcg
     2641 aggcgaaggc ttgcgggggc ggcgtcactt cgaaggcgtt gaaggcgtgg ccgcatctgc
     2701 tcgaagcgtt ggggcgcacg gtggaagagg ttgaaatgta cgcgcccgcc ggtacgcgcg
     2761 tcaggctcaa actcgacgcg cctttcgcca tctactcgcg tgcagctttt gattcctacc
     2821 tgcgcgaccg cgccattcaa gccggagcgc acgttatctc cgaacgcgtc tccgtcgcta
     2881 tggacaacga gaaaaaagag ctttggacgg cgcgcgggcg aagcggtgcg cgttggcagg
     2941 gacgcgtgct cgtcgcggct gacggcgcga acagtccgat tgcgcgtcgt ctggcgggcg
     3001 cgttgccgaa cgcggagatg gaagtcgcgt tcggttatcg cgcgccgctg ccgaagaatg
     3061 gcgaggcgcc gacggtcatc gcgttcctgc cgaattgggt gggctacgcg tgggcgttcc
     3121 cgcgcctcga tcatatctcg ttcggcatcg ccacctcgca agacgccttc aaacatcgcg
     3181 cgctcgacgc gctgctctgg gagttcatgc tcggctacta tcgcatgcgc gacgacgcga
     3241 gtgcgccgct ctggtcttcg caggcgaaac cgacgcctga ggacgaacta cgcgatagag
     3301 agatcgaacg taaactgcgc gcttccgtga accgctacgc cgcgcgtatt cccggtcttg
     3361 cgcctgagac gtggaacacg cgccgcgtcg gttcgcagac gtgggcactc ctcggcgacg
     3421 cagcgggctt cgccgatccg gtcacgggcg aaggcattta ctatgcgttg cgttcggctg
     3481 aaattttcgc ggaggctttt ctggaaggag acacggcggc ttacgaagaa cgctggcgcg
     3541 cggatttcgg gcgcgaactg cgccgcgcct ccgccatgcg ccgacgcttc tacggtgaat
     3601 ttttaggtgc gcccttcacc gaccggatga tcgactttgc ccgtctccat cccggcatcc
     3661 ggcgcacctt acgtgaactc gtcgcgggcg atcaaggtta cgtcaacctc aagcgtgcgc
     3721 tcgcacgcag cgcgctctgg ccgctttgaa ctgtctgaaa attttttaac gcagagggcg
     3781 caaagttaaa gacgcaaagt taaagacgca gcgcgacgcc gggaatctcc ttcgcgttac
     3841 cttgcgcccg cgcttttgcg cgctgtgcgt taaaagtttt ctcaactgtt tacggctcgc
     3901 ccggcacagg ctgagtgtaa ggcgcgaggc ggcgtttgag ttcgtcgggg acgggcacgg
     3961 agtcgaaggt gttgcggcgg acggcgacga gaacgaagtg cgcttcggcg acgattgacg
     4021 ggtcgccgtt gcgccgcact tcaaagttca gacgcagcga tttcgtgccg acgcgcccga
     4081 catagacgga gacttcaagc aggtcgtcca actgcgcggc ctgccggaag tcgcattcga
     4141 gatgtgcgcg cggcagccac acgtcgagtt cgtcgaacat gcgaccgtag ggcaggccga
     4201 ccgagcgaaa aagttccgtc tcggcgatct cgaaaaaacg tatgtacgcg ccgtaaaaga
     4261 tgatgcgcgc cgcgtccacg tcgccccacc tgacgcgttc ctcgatgcga aacttccacg
     4321 cttccgtttc tgacttctgt tggctcatcg ctccattcaa tcaacaactc gtcgcgaaag
     4381 tgatgcgtga gttatcaggc tcgataacaa tgctttcaag gttcaagggc atcgagaaaa
     4441 tcaaccagcg cgcggttgaa cccggcgggt cgttcgagat tcgagacgtg ccccgcttcc
     4501 tctatgactt cgagacgtgc gccgggtatc tcgcgcgaca tcgacgcgga atcagccaaa
     4561 ggcgtgaccg catcgagtcg tccgacgacg acgagcacgg gacaatctat ctcgcgcaac
     4621 agttctctct gatctgcgcg gagcgccatg ccgtgcagag ccgctgccgc gcctcgcggg
     4681 tcggtctgca agatcatgtc gcggacgcgg gcgacgatct cagggcgttc cgtctgagtc
     4741 gcaggcgcga acagcttcgg cagcatggcg tcggcgatag actgcatgcc ttgactgagg
     4801 gcgcgggcgg cagtctcttc gcgtccgagc cgcgcttcat ccgtgtctgc ctgcgggcgc
     4861 gtgtcggcga gcaccagcgc gcgcacgcgt tcgggaaaga gccggtagaa ggcgagcgcg
     4921 acgtagccgc ccatcgaaag cccgccgagt acgactcgcg caaggcgcaa ctcgtcgagc
     4981 agcgcggcga catcctccgc catcacctcc atcgtcgccg gagaatcatc atgcgtggcg
     5041 tgcgtctcgc cgtgcccgcg caggtcaacg gtgacgacgg tgtaacgctc ccgcaacgct
     5101 tcgacctgtt cgcgccagag cgtgcggttg aacggaaagc cgtgcagcaa cacgaccggc
     5161 agccccgcgc ccgacgcctc gtaagccaac tcgattccgc gcacatgtac aacgctcatc
     5221 cggttttcgt ctccctcaaa tctctttccg gccttgcctc tcccgttctt tacttctttg
     5281 cgagaagaac gtctcgccgg gttaagacca aaggcgcaaa agctcgaatg cttgttcgcg
     5341 gctttcagtc tctataaaat tgagccggat tgaaaggcgt agataacatg cgtgtcgcgt
     5401 gagcgtcaaa cgcgcggcgt cgtgcgtgca tctaaactac ggacaacgtt agtgtgattt
     5461 cccggtcacg ccgaaaagtt aaaactcaca gaaggaagca aacatgagca gcaagaaaca
     5521 gacaacgaag gcgcagattt tttcggcggc agagcagcgc gcggcgcgtt ccgagcggat
     5581 cgaggtgagg gaagcggagc cttacggctg gatcgctttt tccgagtcgg gcacggagca
     5641 taactaccac ctgttccgcg accccgaaac gaaacgactc gcctgcacct gcgctgactt
     5701 catctatcgc ggcgacgccg aaccgcgtta cgagtgcaag cacgtctcgg cgacgctcaa
     5761 gtacatcgcg cggcaatatc tcgaaagaga ttacgatccg caacagcagt acgcacgcac
     5821 ctctgccgcg ttggaatcac accacagcac gcggcgcgcg gcctgacttt cccgcgcctc
     5881 aacctgccgc gagaacctcg cggtaaactt tttccgtcgc ttcaaccatt tgttccagac
     5941 tgaaacgagc acgcgcagat tcgcgtgctc gttcgcttat gcgcgtgcgt tcgtcgtcgt
     6001 ttcgtagcag ctcaagcatg gcgcgggcga gcgcgtccgc gtcgccgatg ggcacgagtt
     6061 ttcctgtcac gccgtcttcg ataagttcgc gtgcgccgtc ggttccggtg gcgacggtgg
     6121 cgatgccgca catcaacgct tcgagcgtcg ccagcccgaa agcctctgcg cgcgaggcgg
     6181 agacgtagag gtccagagag ttgatgagcg gcgtgagttc ggagacgtag ccgaggagcc
     6241 ggacgtgtcc ttgtagttcg tgttcggcga tcaacttctc aaggagcgtg cgcgtcggag
     6301 cgcgcggcga cctgtcttcg ccgatgatga gaaactgcgc ccgcgaaaaa ttctcgcgcc
     6361 ggatgatcga cgcagccgcg cgcacaaact cttcctgccc tttaaccgga ctcaactccc
     6421 cgattgtacc cacgatcaac gccgcgctcg caggctgtag tgtctgccgg aatcgtgcgc
     6481 ggtcgaaatc tttcagtgcg gtgtcgagtc ccgcgacgga gatgccgttc gggatgacgc
     6541 ggatcttttc gccggggaaa attcgttgct ggcgtaagcc gcgcgcgacg ccttcggaga
     6601 cggcgatgac gcgtgcgacg ttggcgagtg tgaacctgtg caggcggttg agcgcaaaag
     6661 ggacgtggcg cgtgaggacg aacggcacgc gctctccggc gcggcgcgcg gcgtaggcgg
     6721 cgagcgggta gtcgcgcgcc agatgcgcgt ggacgagttc gatctcgtgg tcgtgtatga
     6781 agcgcgcgag ttgcgacgcg ctcgcgaggt cgagagcgtt gcgaagcggc agcgtggaga
     6841 tgttcgcgcc ggggatgcgc gtgagttttt cgcgcagggg agaagcggga gagagcgcgg
     6901 cgtagacctc ttggccgcgt tcggagagtg cgttcgagag gtctgcgaag tggcgttctc
     6961 cgccgccaaa cgtgcgcgcc gaactgagtt gcagaatctt cactatgaga g
//
LOCUS       DASWNS010000471         4748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_433826, whole genome shotgun sequence.
ACCESSION   DASWNS010000471 DASWNS010000000
VERSION     DASWNS010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433826"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..>4748)
                     /locus_tag="VGB76_16240"
     CDS             complement(251..>4748)
                     /locus_tag="VGB76_16240"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEY0100496.1"
                     /translation="SLNFGAATSPRTARINLTIDAGTTLALSTAAGGDIKIGGNWTRA
                     GTFTPNNRAVFFNGSGAQTITVSPAGSETFNYLVIDKLAGSMMLNSSPATSVLVNATA
                     GDVFQLLSASNFDLNGQSLTMQNNGGNLLVSGGIHVINGSGNFDFTGSKTVTQTGGGT
                     LVFDGATNVTALAAVNFGAGLTTINGTLTLGSGGSVNTNPPTYGSSSTLIYDCTCVYG
                     RSAEWSATSGPGYPNNVTVKTNTDVNIGNTTPATPRQIAGTLNAKSGGNFLMDHPSSP
                     MTAAVTVLKNVLIESGGGLHLSTSSGGDLHVQGDFTNDGTFTHNNRAVFFEGGATGNV
                     NAASGTLTMPYVRINKSGGTVLLGNTNLTTLGDAGGNSLDITSGTLTLNSKMLTLGST
                     VNFAAGTGLIGDTNATLSLQDGGTPGAMGTLIFASGGQSLGNLNINRTGAAGSATVGS
                     DLTLQSALTLTAGDILTGAFTLTHHGASSGTTDVVGNVRRTDLGGTVRQFGNPNNQIS
                     FQTGSAPGEITVNLVKSVPSGSMGFPVAVKRTYTITPTGGSGYTATLRLHYNDSDLNG
                     NLEAALDFWRFNGTTWNRIPKLTADPLAGNWIESPAVTQFSPWTLSAMPLAPTAVKLA
                     SFEAESFADGVEVRWRSGFEVDNLGYNLYRELGGRRTLVNESLIAGSALRVGEGTVLA
                     AGGAYEWFDREGSTSARYWLEALDLNGELQTFGPIVPLIGKEDGKQSVRRQSMLLSEI
                     NNRLTGGDVAQRGWPVSSGGDAAASRNVSVLSKKSVKLAQDSAASVERQWQIANGTAV
                     KIQVNRAGWYRVTQEQLIGAGLDPNSNLEYLQLYADGVEQSMRVSGGSGQGARLLGEG
                     GSIEFYGTVAQTATTGVRTYWLVSGTRPGKRITLASRKGRIASQDKSAMQPVAANGLP
                     EEATDGSSLLHDGRGFDYTVERKERRIYFSSLLNGEKENFYGRVVSTQPVTQALTVRN
                     LVQGSGDGQPQSARLEVALQGVSAGQHQVRVFFNDVELGKLTFADSQSQTAKFNLPAG
                     LVKEGDNLVKFAIVGGTGPDVSLTDYVRLTYAHKFTADTDVLRLRTNTKGTLRIDGFT
                     SPLVRVFDVSDPANVFEIPASPDPVQGASHAVLIESRFGRELLATTEERAEAVSSLAP
                     NRVSAWHTAEHEADLLILTHGTLLGESVEALKAKREEQGMRVSVVDVEDVFDEFSYGA
                     HSPAAIKDFLRWTTTHWKSSPAYLLLLGDASLDPKNYMGRGQFDLVPTKLIDTINMET
                     ASDDWLADFDDDGTAEMFVGRLPVRTAAEANLVVSKIVGFTSGATSNTAVLVSDNPRD
                     GYDFAAASQQAQGLLPAGMEVTSIKRADGTAESVRNRIVQSINLGPQVVSFFGHGSTD
                     VWTGDGLLRGTDAANLTNGNRLPLFVMMTCLNGYYQDPALDSLSESLLKAREGGAIAV
                     WASSGMTVPNEQEKVNRELYRQLFGAQGLTLGEAIGRAKQATEDNNVRRTWILFGDPT
                     LKLR"
BASE COUNT          823 a         1482 c         1467 g          976 t
ORIGIN      
        1 catttcaaat cagtagacac cccgccccct cgatttcctt cgtagccgct ccttagccct
       61 tctcccgcag ggagcgcaat cctcattaac ccgacttcgg catcccttcc cgccacatac
      121 ggcggcgaca gttcgagcat ctatatcttt aatctcaact aaaaatttcc ctccggaaca
      181 aaaaggtgcg gcgggaaaac cgtttctccc gccgcacctt ttactgccgg gtaacacaat
      241 ccactccaac tcagcgcagt ttaagcgtcg ggtcgccgaa gaggatccac gtgcgccgca
      301 cattgttgtc ctccgtcgct tgcttagccc tcccgatagc ttcgccgagc gtcaacccct
      361 gcgcgccgaa gagttgccgg tacaactctc gattgacctt ctcctgctcg ttcggcacgg
      421 tcatgcctga agaagcccag accgcgattg ccccgccctc tctggccttg aggagcgact
      481 ccgacaggct gtcgagtgcc gggtcttgat agtagccgtt caaacaggtc atcatcacga
      541 agagtggcag gcggttgccg ttcgttaagt tggcagcgtc cgtgccccgg agcagtccgt
      601 cgcccgtcca cacgtccgtc gagccgtgtc cgaagaaact caccacctgc ggtcccaagt
      661 tgatgctctg cacgatccgg ttcctgacag attctgccgt cccgtccgcg cgcttgatcg
      721 aggtgacctc catgcctgcg ggcaggagcc cttgcgcctg ctggctcgcc gccgcgaaat
      781 catacccgtc gcgcgggttg tccgaaacca agaccgccgt gttgctcgtc gcgccgctcg
      841 tgaacccgac gatcttcgag acgacgagat tagcttccgc agcggttcgc acaggtagac
      901 gcccgacgaa catctcggcg gtgccgtcat cgtcgaagtc ggccagccag tcgtcggagg
      961 ccgtctccat gtttatggtg tcaatgagtt tggtcgggac gagatcgaac tggcctctac
     1021 ccatgtagtt cttcgggtcg aggctggcgt cgccgaggag caacaggtag gccggagagg
     1081 atttccagtg cgtcgtcgtc catctcagga aatctttgat cgcggcgggc gagtgcgcgc
     1141 cgtaggagaa ttcgtcgaag acatcttcga catctaccac ggacacgcgc atgccctgct
     1201 cttcgcgctt cgccttgagg gcttccacgc tctcgccgag cagcgtcccg tgcgtcagga
     1261 tgagcaggtc cgcctcgtgc tccgcagtgt gccacgcgga cacccgattg ggcgcgagac
     1321 ttgaaaccgc ctccgcccgc tcctccgttg tggcgagcaa ctcccggcca aagcgagact
     1381 cgatcaggac ggcgtggctc gcgccctgta cggggtcggg cgaggccggg atttcgaaga
     1441 cgttcgccgg gtctgacacg tcaaagaccc gcaccaacgg cgaagtgaag ccgtctatgc
     1501 ggagcgttcc cttggtgttg gttctcaggc ggagcacatc cgtgtccgcc gtgaatttgt
     1561 gcgcgtaggt caggcgcacg taatcggtca aactcacatc cggccccgtc ccgccgacga
     1621 tggcgaattt cacgaggtta tcgccctctt tgaccagccc cgccggaagg ttgaatttag
     1681 ccgtctgcga ttgactatca gcgaacgtta gtttgccgag ttcgacatcg ttgaagaaga
     1741 cgcgaacctg atgctggcct gcggagacgc cctggagtgc cacttcaagc cgggcgctct
     1801 gcggctgacc atcgccgctg ccctgcacta gattgcgcac ggtgagcgcc tgcgtgacgg
     1861 gctgcgtgct gacgacccgc ccgtagaagt tctctttctc tccatttagt aaggacgaga
     1921 agtatatgcg tctctccttc cgctccaccg tgtagtcgaa gccccgcccg tcatgaagga
     1981 gcgacgaccc gtccgtcgcc tcttccggca gaccgtttgc ggctacgggc tgcatggcgg
     2041 atttgtcttg agacgcgatg cgccccttcc gtgaggcgag cgtgatgcgc tttccagggc
     2101 gcgtcccgct cacgagccaa taggtgcgca cgcccgtcgt cgccgtctgc gctacggtgc
     2161 cgtagaactc gatggaaccg ccctcaccga ggagccgcgc accttgcccg ctgccgccgc
     2221 taacgcgcat cgactgctcc acgccgtccg cgtagagttg taggtattcg aggttcgagt
     2281 tcgggtcaag tcccgcgccg atgagttgct cctgcgtgac gcgataccag cccgcgcggt
     2341 tgacctgaat cttcacggct gtcccgttcg cgatctgcca ttgcctttcg acgctggcgg
     2401 cggaatcctg agccagtttc acgctcttct tcgagaggac ggaaacatta cggctcgccg
     2461 ccgcgtcgcc gccggaactg accggccacc cgcgctgcgc aacgtcgcct cctgtcaagc
     2521 gattattaat ctcgctcagc agcattgact ggcggcgcac ggactgcttg ccgtcttctt
     2581 tcccgatgag gggaacgatg ggaccaaagg tctggagttc gccgttcaag tcgagggctt
     2641 cgagccagta gcgtgcgctg gtcgagcctt cacggtcgaa ccactcgtaa gcgcctccgg
     2701 ctgcgagcac tgtgccttca cccacacgca gcgccgaccc ggcgatgagc gattcgttga
     2761 cgagcgtgcg ccgcccgccg agttcgcggt acagattgta gccgaggttg tccacctcga
     2821 aaccggaacg ccagcgcacc tcgacgccgt ccgcaaaact ctcggcctcg aacgaagcca
     2881 acttaaccgc tgtcggtgcg agcggcatcg ccgaaagagt ccagggcgag aactgtgtga
     2941 cggcgggcga ctcgatccag ttgccagcta aggggtcggc ggtgagtttg gggattctgt
     3001 tccacgtagt gccgttgaac cgccagaaat ctagtgccgc ttcaagattg ccgttcaaat
     3061 cggaatcgtt gtagtgaagg cgcaacgtcg cggtgtagcc actgcccccg gtcggcgtga
     3121 tggtgtaagt ccgtttgacg gcgacgggga aacccatcga gccggacggg actgacttga
     3181 cgagattaac cgtgatctcg ccgggcgcac tgccggtctg aaaactgatc tgattattcg
     3241 gatttccgaa ctggcgcacc gtcccgccga ggtctgtgcg gcggacgttc ccgaccacgt
     3301 cggtcgtgcc gctcgaagcg ccgtggtgtg tgagcgtgaa ggctccggtc aggatgtcgc
     3361 ccgccgtgag cgtgagcgcg ctttgaagtg tcaggtcaga gccgaccgtg gctgaacctg
     3421 ccgcgccggt gcggttgatg tttaggttgc cgagagattg cccgccgcta gcgaagatga
     3481 gcgtgcccat cgcgccgggc gtgccgccat cctgcaagga gagggtggcg ttcgtgtcgc
     3541 cgatgagtcc cgttccggcg gcgaagttga cggtcgagcc gagcgtcagc atcttgctgt
     3601 tgagcgtgag cgtgcccgaa gtgatgtcga gcgagttgcc gccagcgtcg ccgagcgtcg
     3661 tcaggttcgt gttccccaac agcacagtgc cgccgctctt gttgatgcgg acgtagggca
     3721 tcgtcagcgt gccgctcgcg gcgttgacgt tcccggttgc gccaccctcg aagaagacag
     3781 cgcggttgtt gtgcgtgaac gtcccgtcgt tggtaaagtc gccctgtacg tggaggtcgc
     3841 cgccgctcga agtcgagagg tgcaggccgc cgccgctctc gatgagaaca ttcttaagca
     3901 cggtgactgc tgccgtcatc gggctggacg ggtgatccat caagaaattg ccgccactct
     3961 tggcatttag ggttccggca atctggcgcg gcgtggcggg cgtggtattg ccgatattga
     4021 catcggtgtt ggttttgacc gtaacgttgt tcgggtagcc ggggccgctc gtcgcgctcc
     4081 attcggcgct gcgaccatag acgcaagtgc aatcatagat gagcgtcgag ctgctgccgt
     4141 aggtcggcgg gttggtgttg acggagccgc cgctaccgag ggtgagcgtg ccattgatgg
     4201 tggtcagtcc cgcgccgaag ttcaccgccg cgagggcagt aacgttggtt gcaccatcga
     4261 agacgagcgt gccgccgccc gtctgtgtga ctgtcttcga gcctgtgaaa tcaaagttgc
     4321 cgctgccatt tataacgtgg atgcccccgc ttacgaggag attgccgccg ttgttctgca
     4381 tcgtcagcga ctgcccgttc aaatcaaaat tggacgcgct cagaagctgg aacacgtcgc
     4441 ccgccgtcgc gttgacgagc acgctggtgg cggggctgct gttgagcatc atgctcccgg
     4501 ccagcttatc aatgaccaga tagttgaacg tctcgctccc ggcagggcta acggtgatgg
     4561 tctgcgcgcc agagccgtta aagaagacgg cgcggttatt cggcgtgaac gtgcctgcgc
     4621 gcgtccagtt gccgccgatc ttgatatcgc cgcccgcagc ggtcgagagc gcgagtgtcg
     4681 tgcctgcatc aatcgtcaga ttgatgcgtg cggtgcgcgg cgaggtagct gcgccgaaat
     4741 taaggctt
//
LOCUS       DASWNS010000472         7155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_95680, whole genome shotgun sequence.
ACCESSION   DASWNS010000472 DASWNS010000000
VERSION     DASWNS010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95680"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2047
                     /locus_tag="VGB76_16245"
     CDS             <1..2047
                     /locus_tag="VGB76_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100497.1"
                     /translation="YPDLDALPIDKKRGEITDLDIPSDRVVSFIMDHPLLVITGGDQS
                     GKTSMAKMLYQDMHDRGLVPIILNGEDIRNIDNDSLLKLADREVGKQYDTSLTERYAQ
                     LAPQHKLLIIDDFDHTTIKSRQGHNSIINAIRKHYKRIVIFAGDIFQLSEVAQGSDDE
                     SAFNAFTHVEIKEFGLQLRNRLITKWVMFDRDFTVEDIEIEHQVQEYANNVSTLLLRK
                     TVPSYPVIILSMLQMFDLNHEIAADKGEFGYFYEAYIIQKMAKSKTPSIPMSTIDGYT
                     TELAYKLFMAKTRTISVTQLAAFTEEYRKTYSMNVPQDTMMGVLERGEILKRDGDGSY
                     RFKHKFIYYYFVAKHIKSNIYRESEKARLRGQVSDMAQRIYVEDFYFILMFLVYLTND
                     EEVIEQLLGNGKAFYADLDPCDLDKHVQHINNLDVTLPPLMLNSGDTRKHRDEYEQAR
                     DQAESLRIRSNSDEIGDLEEERDLDEIMRLNVAFRTLQIMGQVLRNFPGVLKGDIKRR
                     LAQESYLLGLRILKFMLVVIEQDTEYFREVAVELVKEFSDVRDMEQLEKKSNQLLFLL
                     QAGLGHSVIKKISQAVGSKHLKETYREVLTANNQISTQLIDTAIKLDHFIDFPKKEVE
                     GLYEKVDRNRFALFILRHMVRDRLYLFSENRKLRQSICDLLGIPINDPKIIEGKNKR"
     gene            complement(2085..2279)
                     /locus_tag="VGB76_16250"
     CDS             complement(2085..2279)
                     /locus_tag="VGB76_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100498.1"
                     /translation="MAIKQTKTELAAVMAFAYSSLAGLSKNTRVLSGSQDSERAARVA
                     RIVAKAENRRRARNAKKAKL"
     gene            2754..3947
                     /locus_tag="VGB76_16255"
     CDS             2754..3947
                     /locus_tag="VGB76_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0100499.1"
                     /translation="MLIKKVKSKRARGDVWMFDFQYRGKRYRQSNFGSRKLCDEVAHA
                     LHSKLQRQHFGLEAAEGSRVTLAELITERLKRPGDPTQQQKRERYFQTFAAMLEPKIR
                     VQEITTQHYGAFVEARCQVVKLQTAFRELTEIVSMINSARDYFPSLENWTPPRRPRLR
                     IPSGQRERIITSDEAARILAELRCPREEGERERTYGLRLDAADLLQIALQTAARRMEI
                     MMLRWSDVNFEWHTLRVVGKKTEHKGDHVRVIPLADSLLELFKRRHRETMGSDLVFPI
                     MLSHSMFNSQTNLIFEVASKRAGIPYGRDTAGGWVLHDARHTAITAMLHAGNSLESVM
                     SISGHHAKTMVMRYSHSTEKTRRAAISALDGFGGDISSGKVANSENGAGSVGTARRKI
                     ARKSA"
     gene            complement(4207..5166)
                     /locus_tag="VGB76_16260"
     CDS             complement(4207..5166)
                     /locus_tag="VGB76_16260"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0100500.1"
                     /translation="MSFIGKSLMTLAAGVILCGAVQTRAQGAASPYRSNESVRSDKFT
                     GGTIERAALVAGEGEIEITVDVPAFRLTLWQNGKEVKTYAIGVGKKDFPISIGEKQAS
                     EIIWNPDWIPPNSEWVTGRKGVKAGEVIRASDPRNPLGKVKIPLGYGYLIHQAAAVTD
                     LGNLVSHGCIRMLRTDLYDLAEKIVAARGVPLPAKKIANAKRTKNTVVAKLEEPLPVD
                     INYDTQVVEGGALHLYPDVYERGTNTVEKLRAELVESGMDASALDDETLKRMLARAKG
                     KQKFVVSLDSIAAGRALTDGRMLPIVGSAATTKKKVVTKRRAA"
     gene            complement(5322..6869)
                     /locus_tag="VGB76_16265"
     CDS             complement(5322..6869)
                     /locus_tag="VGB76_16265"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HEY0100501.1"
                     /translation="MHGYIAAWLAIRMLFRPRQPVKLFGLTVWPQGMIPRHRERLAET
                     IGNAVGNELVSHDTVTDALFETDFFRRKVADLVGTYTHDLLDTPRGSFIEALPAGVRA
                     PVLDAISALELRVADYVTGIMRSEETAQAVNSFIDRRVDELLARRLSDAVDDETYEQI
                     IRFVEDRFHNIVNERGFEGKVRDFVSARVEELAASRATLAEVFTPEAVAIIKERIDAQ
                     LAPIAEHLAEIASNKNTRTQIGALIKREVDEYYAQLGFFKKIFVSRERIHGEVDEMVN
                     KTLPRRVQEFLRGEAFAQEAETFLNSMIDNLLARPINELVGTITPEKLELIKDQIASR
                     ILVIARGAELSKTVSAYTSDALARVRPHTIRALLEHASPDSAARLKNFLSRALLGVLA
                     REDTARTINAILSAQIERLLLAPIGRISDHMPEQSITRATSALTERITSVARERLPIA
                     IKEFDIGGIVRTKVSNYPVEKLEALVLSVAQQHLRKIELFGAIIGLFLGLSQAIYFGL
                     KYYRAGP"
BASE COUNT         1698 a         1840 c         1977 g         1640 t
ORIGIN      
        1 ctatccagat cttgacgctc ttcctataga caaaaaacgt ggtgaaatta ccgatctaga
       61 tatcccttcc gatcgtgttg tttcattcat aatggaccat cccctgcttg tcataactgg
      121 gggcgatcaa tcgggtaaga cttcaatggc aaagatgctt taccaagaca tgcatgatcg
      181 tggcttggtg cccattattc tcaatggaga agatataaga aatatagaca atgatagctt
      241 actaaaactc gccgaccgcg aagtcggaaa acagtacgac acctccttaa cagaacgata
      301 cgcacagttg gccccgcagc ataaattgct aatcattgac gatttcgatc ataccaccat
      361 taaaagccgt caagggcaca actccattat taacgcgata cgcaaacact ataaacggat
      421 cgtcattttt gctggagaca tttttcaact gtctgaggtg gctcagggtt ccgacgatga
      481 gagtgccttc aacgcattta cccatgtaga gataaaagag tttgggttgc agcttcgtaa
      541 tagactgatt acaaagtggg tcatgtttga ccgtgatttc acagtggagg acatagagat
      601 agaacatcaa gttcaggaat acgctaacaa tgtttctact cttctattgc gtaaaacagt
      661 cccgtcctat cctgtaatta ttttgagtat gctgcaaatg ttcgacctta atcatgagat
      721 agcagcagat aagggggagt ttggatattt ttatgaagct tacattatcc aaaagatggc
      781 aaagagcaaa acgccatcaa tcccaatgag tacaatagac gggtacacaa cagaacttgc
      841 ctacaaattg ttcatggcaa aaacccgcac aattagtgtt acccaactag cggcttttac
      901 ggaagaatac cgtaagacat acagcatgaa tgtccctcaa gacactatga tgggagtgct
      961 agaacgtggc gaaatattga agcgggatgg cgatggttcc tatagattca aacataagtt
     1021 tatttactac tactttgtag caaagcacat aaaaagcaac atttacagag agtcggagaa
     1081 agcaaggtta agaggtcaag tatccgacat ggcacaaaga atctacgttg aggatttcta
     1141 cttcatttta atgttcttag tttatctcac caatgatgaa gaagtaattg agcaattatt
     1201 gggtaatgga aaagcctttt atgctgacct agacccttgc gatttggata aacatgttca
     1261 gcatataaac aatcttgacg taaccttacc tccacttatg ctgaacagcg gtgatacgcg
     1321 aaaacatagg gacgagtatg aacaggcacg ggatcaagcg gaaagtctaa ggataaggag
     1381 taattcagat gagattggag acttggaaga agagcgagat ctggatgaaa taatgcgctt
     1441 aaacgtggct tttagaactc ttcaaattat gggtcaagtg ctgcgcaatt tccccggtgt
     1501 attgaaggga gatattaagc ggcggttagc acaagaaagc tatctattag gattgcgtat
     1561 acttaaattt atgcttgttg tgattgagca agatacggag tacttcagag aagtagcagt
     1621 tgaattggtg aaggagtttt ccgatgtcag agatatggag caactggaaa agaaatccaa
     1681 ccaattatta ttcttgttgc aagccggcct cggacattct gttattaaaa agatttccca
     1741 agcggttggt tccaagcatt tgaaagaaac ctatcgtgag gtcttaacag caaacaacca
     1801 gatatcaaca cagttaattg acactgccat caagttggac cacttcattg attttcccaa
     1861 gaaggaagtt gaaggattat acgaaaaagt tgacagaaat cgcttcgcac tttttatttt
     1921 gcgacatatg gttagagatc ggctctatct tttctcagaa aaccggaaac tgcgccaaag
     1981 tatttgtgac ctactaggta tccctattaa tgatcccaaa ataattgaag gtaagaacaa
     2041 gaggtaggat ttactagatg gatactaagg gcttaacgct ccatttaaag ctttgccttt
     2101 ttagcatttc ttgctcgtct tctgttttct gcctttgcaa ctatgcgagc aactcgcgct
     2161 gccctttccg aatcctgaga gccagataaa actctagtgt tttttgaaag accagccaaa
     2221 gatgagtagg cgaaggccat cactgcagcc agttctgttt tagtctgttt gatagccata
     2281 actcaaatcc agtttagttg ggaacttgta attttacttc aacagggaaa ttcttacaag
     2341 tctgaaacaa tcgtgattgt gaagtcattt tcactcttga actaatagag ataagtcatt
     2401 actgagaact agaatcctta tctttgtatg ctcattatcg aaaaaaggtt tcagtgtagc
     2461 taacaaagga gggcattgtt agccaaagag cgaggcatcg tgcccgtaca agcggcaata
     2521 tccatgccaa cacatctggt ctcttttagg cgaagaatag actttagtca caggtagatt
     2581 aaataagatg tctataagat gaggtcggat ttgagcagca gatgagaact tttgttgaca
     2641 cttacttttt gtctaatgct gaaaatgatc ttctaccatt atcgtagata ttaactgcct
     2701 tcgtattcag agataccacc gcaactcccg gggcggctca catcaggtaa accatgctca
     2761 tcaagaaggt caaaagtaaa cgcgcacggg gtgacgtctg gatgttcgac ttccagtacc
     2821 ggggtaagcg ttaccgccag tctaacttcg gctcccgcaa actctgcgac gaggtggcgc
     2881 acgccctgca ctccaaactc cagcgacagc acttcggact cgaagccgcc gagggctcgc
     2941 gcgtcacgct cgccgagttg atcaccgagc ggctcaagcg ccccggcgac cccacgcagc
     3001 agcagaagcg ggaacggtat ttccagacct tcgccgccat gctggagccg aagattcgcg
     3061 tccaggagat cacgacccag cactacggcg ccttcgtcga ggcgcggtgc caggtggtca
     3121 agctccagac cgccttcagg gaactcaccg agatcgtctc gatgatcaac tcggctcgcg
     3181 actacttccc gtcgctcgag aactggacgc ctccgcgacg gccccgcctc cgcatcccct
     3241 ccggccagcg cgagcgcatc attaccagtg acgaggccgc gcgcatcttg gcagaactca
     3301 ggtgcccacg tgaggagggc gagcgcgagc gcacttacgg cctccggctc gacgccgccg
     3361 acctcctgca gatcgcgctc cagaccgccg cccgccgcat ggagatcatg atgcttcgtt
     3421 ggtcggatgt gaacttcgag tggcacacgc tccgggtcgt gggcaaaaag acggagcaca
     3481 agggcgacca tgtgcgcgtg attccgctcg ccgacagtct cctcgaactg ttcaaacgaa
     3541 ggcaccgcga gacgatggga agcgatctcg tcttccccat catgctctct catagcatgt
     3601 tcaactcgca gacgaatctg atcttcgagg tcgcctcgaa gcgcgcgggc atcccttacg
     3661 ggcgcgacac ggcgggcgga tgggtcttgc acgacgcgcg ccacacagcc atcacggcga
     3721 tgctgcacgc gggcaacagc ctcgagagcg tgatgagcat cagcggccat cacgccaaga
     3781 cgatggtcat gcgctactcg cacagcacgg agaagacgag gcgcgcggcc atctcggcac
     3841 tcgacggatt tggtggcgac atttcgtccg gaaaagttgc gaacagtgag aatggtgcgg
     3901 gtagtgtggg aacagcccgg cgaaaaatcg ccaggaaatc tgcgtaaaac acgataaccc
     3961 gtgacaatgc aatagctagc taggcagcgt gcgaggcatg gcactgggaa tattgcgcgc
     4021 cataactccg gcacgggcgg aatcttcaac acgtagaact caaagcagaa gacacgaagg
     4081 acgcgtagaa gattcgttct ccgcgtcctt cgtgtctctg cgtgttttgc gtgtctctgc
     4141 gtgttctgct tccgcgtgcg ctacgttaaa agacttcttc gccgccgcgt cgcgttcaat
     4201 gaagcgttaa gccgcgcgcc gcttcgtcac gactttcttc ttcgtcgtcg ctgccgagcc
     4261 gacaatcggg agcatgcgcc cgtcggtcaa agcgcgtccc gcggcgatgc tgtccagact
     4321 gacgacgaac ttctgcttgc ccttcgcgcg cgcgagcatc cgcttgagcg tctcgtcgtc
     4381 gagcgcggac gcgtccatgc cgctctccac caactccgcg cgcaacttct cgaccgtgtt
     4441 cgtgccgcgc tcgtaaacgt ccgggtagag atgaagcgcg ccgccttcaa ccacctgcgt
     4501 atcgtaattg atgtcaaccg gcagcggctc ttcgagtttg gcgacgacgg tgttcttcgt
     4561 ccgcttcgca ttcgcgatct tcttcgccgg gaggggcacg ccgcgcgcgg ccacgatctt
     4621 ttcggccaga tcgtagaggt ctgtgcggag catgcgaatg cagccgtgcg agacgagatt
     4681 gccgaggtct gtcacggcgg cggcctgatg aatcaggtag ccatagccga gcgggatctt
     4741 cactttgccg agcgggtttc tagggtcgct cgcgcgtatg acttcgcccg ccttcacccc
     4801 tttgcgtccc gtcacccact cgctgttcgg cggaatccaa tctggattcc agatgatctc
     4861 cgacgcctgc ttctcgccaa tcgagatcgg aaaatccttc ttgccgacgc cgatggcgta
     4921 agtcttcacc tctttgccgt tctgccacag cgtcaggcga aacgcgggca cgtcaaccgt
     4981 aatctcgatc tctccttcgc ccgcaaccag cgcggcacgc tcaatcgtcc cgcccgtaaa
     5041 cttatccgag cgcaccgatt cattggaacg atacggcgaa gccgctccct gcgcccgcgt
     5101 ctgaaccgcg ccgcacaaga tgacccccgc cgccaacgtc atcaaacttt taccgataaa
     5161 actcatcttc tgctctcttc tcctcaggtc ttccgctgac tccgcgcaca cagttgtcgc
     5221 cgaggtcagg atcatcgcgc gatttctatg tggggagtga gtcgttagtt tgtgtgctga
     5281 cgcgcccgaa tggttgcctg ttaaagcata acaactccgc ctcaaggccc cgcgcggtaa
     5341 tatttgaggc cgaaatagat ggcctgtgag aggccgagga agaggccgat gatcgcgccg
     5401 aagagttcga tcttgcgcag gtgttgctgt gcgacggaga gcacgagcgc ttcgagtttt
     5461 tcgaccgggt aattcgagac tttggtacgc acgatgccgc cgatgtcgaa ctctttgatg
     5521 gcgatgggca ggcgttcgcg cgcgacggaa gtgatgcgtt cggtgagcgc gctggtggcg
     5581 cgcgtgatcg attgttcggg catgtgatcg ctgatgcgcc cgatgggggc gaggaggagg
     5641 cgttcgatct gcgcgctcag gatcgcgttg atggtgcgcg ccgtgtcttc gcgtgcgagc
     5701 acgccgagca aggcacggga gagaaagttt ttgagccgcg cggcggaatc ggggctggcg
     5761 tgttcgagga gcgcgcggat ggtgtgcggg cggacgcgcg cgagcgcgtc ggacgtgtag
     5821 gccgagacgg tcttcgagag ttccgcgcca cgtgcgatta ccagaatgcg cgacgcgatc
     5881 tgatctttga tgagttccag cttttcgggg gtgatggtgc cgacgagttc gttgatcggg
     5941 cgcgcgagca ggttgtcgat catcgaattg agaaacgttt cggcctcctg tgcgaaggct
     6001 tcgccgcgca aaaattcctg cacgcggcgc gggagcgtct tgttgaccat ctcgtcaact
     6061 tcgccgtgga tgcgttcgcg cgagacgaag atctttttga agaagccgag ttgagcgtaa
     6121 tactcgtcaa cttcgcgttt gatgagcgcg ccgatctgcg tgcgcgtgtt tttgttggaa
     6181 gcgatctcgg cgaggtgttc ggcgatgggg gcgagttgcg cgtcgatgcg ctccttgatg
     6241 atggcgacgg cttcgggcgt aaagacttcg gcgagcgtgg cgcggctggc ggcgagttct
     6301 tccacgcgtg cgctgacgaa atcgcgcacc ttcccctcga agccgcgctc gttgacgatg
     6361 ttgtgaaaac ggtcttcgac gaagcgtatg atctgttcgt aagtctcgtc gtccacggcg
     6421 tcgctcaggc ggcgcgcgag cagttcgtca acgcgccggt cgatgaagga gttgaccgcc
     6481 tgtgcagtct cttcgctgcg catgattccg gtgacgtaat cggcgacgcg caattcgagc
     6541 gcgctgatgg catcgaggac gggcgcgcgc acgcctgcgg gcagggcttc gatgaaagaa
     6601 ccgcgtggcg tgtcgagcag gtcgtgcgtg taggtgccga ccaggtcggc cactttgcgg
     6661 cggaagaaat ccgtctcgaa gagagcgtcg gtcacggtgt cgtgcgagac gagttcgttg
     6721 ccgacggcgt tgccgatagt ctccgcgagg cgttcgcggt ggcgcgggat catgccctgc
     6781 ggccagacgg tgaggccgaa gagtttcacg ggctggcgcg ggcgaaacag catgcggatg
     6841 gcgagccacg ccgcgatata cccgtgcacg gtggcgaaga cgaccagacc tagcagttct
     6901 aaaagttgcc agcgattgat gagtgtttat cctctaggct tgcgcgcggc gaaaatcgga
     6961 tagtgtcaga gtggatgcta aacggataga gagaacggcg tcaattggag aagcaaggcg
     7021 aacgatgaag gatgaaggat gaaggatgaa tggaaacgcc cggcgtgcag aggttatcat
     7081 tcatcgttca tccttcatcc ttcatcgttt cctgctcatt ttcccacgtc gaagagtttg
     7141 ccgccgcgct cggag
//
LOCUS       DASWNS010000473         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_458281, whole genome shotgun sequence.
ACCESSION   DASWNS010000473 DASWNS010000000
VERSION     DASWNS010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458281"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VGB76_16270"
     CDS             complement(<1..301)
                     /locus_tag="VGB76_16270"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY0100502.1"
                     /translation="MAVVQRRLSKSFKRFFDSEKSSGILLIICTAVSLLLANSIIGAN
                     YLSIWHTYIGGLSVEHWINDALMAVFFLFIGLELERELYNGELSNFRNALLPIFAA"
     gene            complement(536..1351)
                     /locus_tag="VGB76_16275"
     CDS             complement(536..1351)
                     /locus_tag="VGB76_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY0100503.1"
                     /translation="MRQTSEPTPERPLLDAERRELLVRLEDWLETPMLVLGFLWLALL
                     ILELTRGLSPALEVAGTIIWIVFIVDFILKFALAPDKSDYLKANWLMALALAVPALRV
                     FRVVRVVRVLRAARAARGLRLFRVVSSLNRGMRALGATMRRRGFAYVIALTLIVALSG
                     AAGMYAFENELPDGQGFESYGEALWWTAMVMTTMGSDYFPRTTEGRVLCFALAVYAFA
                     VFGYVTATLATFFVGRDAADAQAELAGTEQVERLQAEVAALREELRAFIRREE"
     gene            1376..1999
                     /locus_tag="VGB76_16280"
     CDS             1376..1999
                     /locus_tag="VGB76_16280"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0100504.1"
                     /translation="MSAISSRITPPPSTDFIGFDLGKVSSQVCIITEDGELIERRIKT
                     DCEHIRELLGSRPLARILIESGTGSEWIARHLEALGHEVIVADPNFASMYATRSRKVN
                     TDRRDARTLAEACRLGAYRPAHRTSDKQRHVRAQLAVREAMVRTRSRYISFVSTLLRR
                     DRLRMHNSGYTPTFLKRLAQLELSELLQAEITPLVFLGSACNLLAQA"
     gene            complement(1955..>2173)
                     /locus_tag="VGB76_16285"
     CDS             complement(1955..>2173)
                     /locus_tag="VGB76_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100505.1"
                     /translation="TKMGGQGAPDDLQKGYETQVWLAISNDEKAKVSVRYFYHRKESR
                     HHPEAVGALLQERFLGLCQEITGASQKY"
BASE COUNT          459 a          700 c          614 g          400 t
ORIGIN      
        1 ccgccgcgaa gatggggagt agcgcatttc tgaaattcga gagttcgccg ttatacaact
       61 cgcgttcgag ttcgaggccg atgaacagaa agaagacggc catcagggca tcgttaatcc
      121 agtgctcgac gctcagccca ccaatatacg tgtgccagat gctcaggtag tttgcgccga
      181 tgattgaatt ggcgagcagg agcgaaacag ccgtgcagat aatcagcagg atgccgctcg
      241 atttctcgga atcgaagaac cgcttgaagg acttcgataa tcttctttgg acaacggcca
      301 taatagtctt ccagccaaag taacaaaaac atttgaattc cgcacgcgac acactcatgc
      361 ccccggcggc gcggacgggc ttgcaccggc gggacttaag cctttagctg ctccgcatga
      421 tcaatagctt aaccctcgcc gctcgtgcgg cggtgcgccg tggagtagcg gcagttcgac
      481 cgtgaaagtc gaacctaaga cttccctctt cgcgtcccgg cttaagtacg ggcatttact
      541 cttcacgccg aatgaaggct cgtagttcct cgcgcagagc ggcgacctcc gcctgtagtc
      601 gctcgacctg ttcggtgcct gccagctccg cttgcgcgtc cgccgcgtca cgcccgacga
      661 agaaggtggc gagcgtcgcc gtcacgtagc cgaagacggc gaaggcgtag acggctagag
      721 cgaagcagag cacgcgccct tccgttgtcc tcgggaagta gtccgatccc atcgtggtca
      781 tcaccatcgc cgtccaccac agcgcctccc cgtaagactc gaagccctgc ccgtcaggca
      841 gttcattctc gaaggcgtac atgccggctg cgcctgacag cgcgacgata agagtcaacg
      901 cgatgacgta tgcaaaccca cgacggcgca tcgtcgcgcc gagcgcgcgc atgccgcgat
      961 taagagagct gacgacgcgg aacaggcgca acccacgcgc ggcgcgagcc gctcgcagga
     1021 cgcgcacgac gcgcacgacg cggaagaccc gcagtgccgg gacggcgagc gccagcgcca
     1081 tgagccagtt cgctttcagg tagtcggatt tgtcaggggc aagggcgaac ttcaggatga
     1141 agtccacgat gaagacgatc cagataatcg tgccggcaac ttcgagcgcg ggcgagaggc
     1201 cgcgcgttag ctccaggata agcagcgcga gccagaggaa gccaagcacg agcatcggcg
     1261 tctccagcca gtcttcgagc cgcacgagca gttcgcgccg ctcggcgtcg agcagcgggc
     1321 gttctggcgt cggttcgcta gtctgcctca ccggtgcctc gaagtgctcc ttaatgtgtc
     1381 agccatctcg tcacgcataa ctccgcctcc ttccacggac tttatcggtt tcgacctggg
     1441 taaagtttcc agtcaggtct gcatcatcac cgaagacggc gaactcatcg aacgacgcat
     1501 caagactgac tgcgagcaca tccgcgaact actcggcagc cgcccgcttg cgcgcatcct
     1561 gattgagtcg ggcacgggga gcgagtggat cgcacgccac ctcgaagctc tcggccacga
     1621 ggtcatcgtc gccgacccta acttcgcctc gatgtacgcc acccgcagcc gcaaagtcaa
     1681 caccgaccgg agagacgccc gcacgctcgc cgaggcttgc cgactcggag cctaccgacc
     1741 ggcgcaccgc acttcggata agcagcggca tgtgcgcgca caactggcag tgcgcgaggc
     1801 catggtgcgc acccgctcgc gttacatctc gttcgtctcc acgctgctgc gccgcgaccg
     1861 actgcgcatg cacaacagcg gttacacgcc cactttcctc aagcgcctgg cacaactcga
     1921 actgtccgag ttgctacagg cggagataac cccgttagta tttctgggaa gcgcctgtaa
     1981 tctcctggca caggcctaga aatctctcct gcaacaacgc accaacagct tcggggtgat
     2041 ggcggctttc ttttcgatga taaaaatatc ggacgctaac ctttgccttc tcatcgttgc
     2101 ttatagcaag ccacacctgc gtctcgtatc ctttttgcag atcatcaggc gcaccttgcc
     2161 cgcccatctt cgt
//
LOCUS       DASWNS010000474         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_122421, whole genome shotgun sequence.
ACCESSION   DASWNS010000474 DASWNS010000000
VERSION     DASWNS010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122421"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VGB76_16290"
     CDS             <1..360
                     /locus_tag="VGB76_16290"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0100506.1"
                     /translation="SEHCITVFTESEAQRIIGAEQVEAIEILRRGAIKPFQMAVGGVL
                     VRVGVEPNAELFSGQLEQDERGYIKVTSEQETSSENVFAIGDISNPRSPTISSATGAG
                     ATAAKVLAARLGDKSRR"
     gene            501..824
                     /locus_tag="VGB76_16295"
     CDS             501..824
                     /locus_tag="VGB76_16295"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0100507.1"
                     /translation="MNYQTISPTEFAERIARGERVRLIDVREPEEFELARVEGAELLP
                     LSRFQEWAGALEAEEEIVVMCHHGIRSAHVCAHLAAQGFTKMVNLAGGIERWSVEVDR
                     SVPRY"
     gene            887..1321
                     /locus_tag="VGB76_16300"
     CDS             887..1321
                     /locus_tag="VGB76_16300"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEY0100508.1"
                     /translation="MAETSDVAYTAEHAAAYPDDYLAGIDLYNAGEFHAAHDAWEARW
                     VDEAGPREKLFLQAMIQSAVAFHHLEIGRRGAARQMYLMAKEKFARLKTPRFMSLDLV
                     DYQAQLDRALAWLVSVPDPRELAMPEIELPSIKLLPEVMEYD"
     gene            1321..1893
                     /locus_tag="VGB76_16305"
     CDS             1321..1893
                     /locus_tag="VGB76_16305"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEY0100509.1"
                     /translation="MEAKLDAQATKAGSLSRARAGDAATTARAGHALGDVRLLLLGLW
                     LGAAMFFSFAVAPGAFAVLPARELAGAIVTRTISVVNVCGFVIGLLLLATAFAGRGAA
                     AARRAWLSEVFALALLVLATGTGHWVVSARMHALRLSMGRPIDELAQSDPLRLAFNDL
                     HGYSVAAMTAAMLAGLFAFILIARRNRNLK"
     gene            1974..>2735
                     /locus_tag="VGB76_16310"
     CDS             1974..>2735
                     /locus_tag="VGB76_16310"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HEY0100510.1"
                     /translation="METIEQIDEWRESYQRWKRINEMRGDDIGEHYPWVENARAPFKP
                     MGRALPMLNLALITSAGAYVDGSEPFDVTLPGGDASFREIPTLIEAEDLRWAARGYDP
                     AAVEQDMNAQVPLERLFEFQGNGIIGQLNPVFWSFCGFIPSAARFADNALPQLVERVK
                     RYSAQAALLIPASRLCHQSMGLAARALEAAGITTIVLAVDKEAIERVRPPRAVYYTGE
                     FGRVAGLPNFPEHQRRILDETLRLLEPWDQVDVRKL"
BASE COUNT          494 a          812 c          919 g          510 t
ORIGIN      
        1 tcggagcatt gcatcacggt cttcaccgag tcggaagcgc agcgcatcat cggcgcggag
       61 caggttgagg cgattgagat tttgcggcgc ggcgcgatca agccctttca gatggccgtc
      121 ggtggcgtgc tcgtgcgcgt cggcgtcgag cctaacgcgg aacttttcag cgggcagcta
      181 gaacaggacg agcgcggtta catcaaggtg acgagcgaac aggagacgag cagcgaaaac
      241 gtgttcgcta tcggcgacat ctccaacccg cgcagcccga cgatcagcag cgcgacgggc
      301 gcgggtgcca ccgccgcgaa agttctggcc gcgcggctgg gcgataaaag ccgccggtag
      361 ccggttgccg cttgttcgtc gccggtggca ggtgtcggtg cccggttgtt ctttcccggt
      421 cgttaagtta aatctttagc cactgacgcg gcggccacag gcgacggaaa actgatcgaa
      481 ggggaactga caatctttct atgaactacc agacgatctc gccaaccgag tttgccgaac
      541 gcatagcgcg cggcgaacgc gtgcgcctga ttgacgtgcg cgagccggaa gagtttgagt
      601 tggcgcgggt ggagggcgcg gagcttctgc cgctcagccg ttttcaggaa tgggcgggcg
      661 cgctcgaagc ggaggaggag attgtggtga tgtgtcatca cggcatccgc agcgcgcacg
      721 tctgcgcgca tctggcggcg cagggtttca ccaagatggt gaatctcgcg ggcggcatcg
      781 agcgctggtc tgtcgaagtt gaccgcagcg tgccgagata ttgaaggaag ctatcagcct
      841 gaacggctac tgacgttttt cgagtttgct tcgtataatg tctgaaatgg cagagacgag
      901 cgatgtggct tacacggcgg agcatgccgc cgcgtacccg gacgattatc tggcgggcat
      961 tgatctctac aacgcgggcg agtttcacgc ggcgcacgat gcctgggagg cgcgctgggt
     1021 ggatgaagcg ggaccccgcg agaaactttt tttgcaggcg atgattcagt cggcggtggc
     1081 ctttcatcat ctcgaaatcg ggcggcgcgg cgcggcgcga cagatgtacc tgatggcgaa
     1141 ggaaaagttt gcgcgcttga agacgccgcg cttcatgtcg ctcgacctcg tagattatca
     1201 agcgcaactc gaccgcgcgc tggcatggct cgtgagcgtg cccgacccgc gcgagttggc
     1261 gatgccggag atcgagttgc cctcgatcaa gttgctgccc gaagtgatgg agtacgactg
     1321 atggaggcga aactggacgc acaggcgacg aaggccggaa gcctctcccg cgcgcgtgcg
     1381 ggcgacgcgg cgacaaccgc gcgcgccgga cacgcgcttg gcgacgtgcg cctgttgctc
     1441 ctcggcctgt ggctcggagc agccatgttc tttagttttg cggtagcgcc gggcgcgttc
     1501 gccgtgctgc ccgcgcgtga gttggcgggc gcaatcgtca cgcgcacgat cagcgtcgtg
     1561 aacgtctgcg gcttcgtcat cggcctgttg ctgctggcga cggcgttcgc cgggagaggc
     1621 gcggcagcgg cgcggcgtgc gtggctgtcg gaagtgttcg cgctcgcgct gctcgtgctg
     1681 gcgacgggca ccgggcactg ggtcgtcagc gcgcgcatgc acgcgcttcg tctctcgatg
     1741 ggacgaccga ttgacgaact ggcgcagagc gatcccttgc gcctcgcctt caacgacctg
     1801 cacggctatt cggtcgcggc gatgacggcg gcgatgctcg ccgggctgtt tgcgtttatc
     1861 ttgatcgcgc ggcgcaaccg caatcttaag tgacgagtga caagtcttaa aataaggtct
     1921 tgacttgtca ctcgtcactt gtcacttgaa agttattccg aaggagatgc ttggtggaaa
     1981 cgattgagca aattgacgag tggcgcgaaa gctaccagcg ttggaagcga atcaacgaga
     2041 tgcgcggcga cgacatcggg gagcattacc cctgggtgga gaacgcacgc gcgcctttca
     2101 aaccgatggg acgcgcgctg ccgatgctca acctcgcgct catcacttcg gcgggcgcat
     2161 acgttgacgg ctccgaacca ttcgacgtga cgctccccgg cggcgacgcc agtttccgcg
     2221 aaatccccac gctcatcgaa gcggaggatt tgcgctgggc ggcgcgcggt tacgacccgg
     2281 cggcggtgga acaggacatg aacgcgcaag tgccgctcga acgactcttc gagtttcaag
     2341 gcaacgggat catcggccaa ctcaaccccg tcttctggag cttctgcggc ttcatcccga
     2401 gcgcggcgcg cttcgccgac aacgccttgc cccaactcgt cgagcgcgtc aagcgttatt
     2461 cggcgcaggc cgcgctgctc atcccggcct cgcgtctctg ccaccagtca atgggactcg
     2521 ccgcgcgcgc cctcgaagcc gcaggcatca cgaccatcgt gctcgccgtg gataaagagg
     2581 cgattgagcg cgtgcgcccg ccgcgcgccg tctattacac gggcgagttc gggcgcgtgg
     2641 cggggctgcc gaacttcccc gaacatcaac gccgcatcct cgatgaaacc ctgcgcctgc
     2701 tcgaaccgtg ggatcaagtt gacgtgcgca aactg
//
LOCUS       DASWNS010000475        16566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_147176, whole genome shotgun sequence.
ACCESSION   DASWNS010000475 DASWNS010000000
VERSION     DASWNS010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16566
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147176"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VGB76_16315"
     CDS             <1..884
                     /locus_tag="VGB76_16315"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY0100511.1"
                     /translation="DSPLAEAKFSQDHLTVLTTLASVAAIRVENARLMEEDLERERFE
                     RELNLAREIQQRFQPSGAPNVNGYEMQGISFPCYEIGGDYYDFIQRPDGRMVIALGDV
                     SGKGTSAALLMSSLHAAMHAQIPAHQTLGETISAVNRYLAETIPLNRFITLFCAELDP
                     ATGTLSFLNAGHNPPLIAHAGGSMEQLAAGGIPLGIVADAVYREGRTQLQPGDALVIY
                     SDGVSETQNAAGEEFGPVRLYNTVARYLDSTAAGIRDKIEADLTKFAQGTPAGDDITL
                     VIVKRQAEGAMVASSRS"
     gene            934..2214
                     /locus_tag="VGB76_16320"
     CDS             934..2214
                     /locus_tag="VGB76_16320"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY0100512.1"
                     /translation="MTSTAQLRRRFLTRFKCSFIAAFVCLSLAPAAAAQQKARARDLG
                     IPFEGRPGTHNAITDVAGVEVGHTTLISGSGRLVRGQGPVRTGVTAVLPRGKKSFDPV
                     FAAWFPLNGNGEMTGTTWIRESGFLEGPILITNTHSVGVVRDATIDWEIRHGRKFLFT
                     YPIVAETYDGVLNDINGFHVKPEHVFSALDGSSGGRVAEGNVGGGTGMLCNGFKGGIG
                     TSSRIVAAEVGGYTVGVLVQCNYGSRRGLRIAGVPVGQEFTDPSICLEGMDAPSQEFL
                     RGMPRCGKSAASLERPHPELGSIIIVVATDAPLLPHQLDRLVKRAALGLGREGSIASN
                     SSGDIFVAFSTANAGAAAERGPITLKMMPNEQLDPLFAATIQATEEAVTNAMLAAETI
                     TGADNVRAEAIPHERLREIMRKYNRLPASPSNAK"
     gene            2266..2649
                     /locus_tag="VGB76_16325"
     CDS             2266..2649
                     /locus_tag="VGB76_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100513.1"
                     /translation="MNKERRSIIKTLSRALRLKCPACGLTSIVQSPFHIKHQCSACDV
                     LFKREAGFFVGAIMANVVTTEFVILLLYVAALPVIDTRFDLVLTCLSVVALIFPVAFY
                     HHSWSLWLGFDHLIETLPKAPKAQS"
     gene            2724..3215
                     /locus_tag="VGB76_16330"
     CDS             2724..3215
                     /locus_tag="VGB76_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100514.1"
                     /translation="MKLSRILFLLLLGCILSEIETGAQATKPVRPPGRGGMWKSRLGC
                     APRKLFRGDSLRLTMSVPHGGDLAIVAPGDGYFLLSFWQPDKTDARQSLFDWEAFKSR
                     RQLTLDTARLKAKPWVAGRDEPEPVFSRTGWYRVRLSENLETDDGTPVSECRVYYVNA
                     KRR"
     gene            complement(3350..4021)
                     /locus_tag="VGB76_16335"
     CDS             complement(3350..4021)
                     /locus_tag="VGB76_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100515.1"
                     /translation="MNGIASNRPSAKQFNNPLPLRHTLLETRMNYPLDISFKLIALAQ
                     QLSVRDAQGNLIFYVKRKAFKLKEALTVFADEAQQRPLYHINADRVMDFSARYRFTDN
                     AGNSLGSIKRHGMRSLWKSHYEIDDDAPGGAVSVTEENAWIKVFDSVIGQLPIIGALS
                     GYLFHPAYLLSRADGTVLLRMEKQPAFFEGKFRIEKRAELDQAEETRALLGLLMLIML
                     ERSRG"
     gene            complement(4018..4464)
                     /locus_tag="VGB76_16340"
     CDS             complement(4018..4464)
                     /locus_tag="VGB76_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100516.1"
                     /translation="MNTTPESLADLFRQLAFVGALIGGFAFAFLGVLLTAPSRSRIVE
                     WTAGTAMATAAGLIVCVIGWTLMASVIVMSAPANGGAEASLQAAAKFNLMHSRLSLLF
                     IICMFLFLASLGLSGWVRSRALGIVSTVLALLAAVGLLLVMSPFLR"
     gene            complement(4597..5055)
                     /locus_tag="VGB76_16345"
     CDS             complement(4597..5055)
                     /locus_tag="VGB76_16345"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY0100517.1"
                     /translation="MENGEIARRFERLALIMEIRGDDRYRVRSYRNASEIIETWPTPL
                     AQIAREEGAKGLQAIPSVGRAISGKIVELCERGTFEAYEKLIAETPETVLDLLDVSGI
                     GIKTAAALHQQFKISSLDDLRKFVAGGGLEMVDGIGEKTAERIAASLKVK"
     gene            5258..7345
                     /locus_tag="VGB76_16350"
     CDS             5258..7345
                     /locus_tag="VGB76_16350"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEY0100518.1"
                     /translation="MNIRKLARTLLAALITFSSFAGFTTARADEGMWPFNQVPRAEIK
                     RKYGFEVTDEFLKKLQLASVRFPNGSGSFVSPDGLVLTNYHIIEDAVGELGSAEKDYA
                     KEGFVARTRAEELKIPGYELNVLQSIEDVTDRVNASVTPAMSAGDAFNARRTAIAAIE
                     KESTEKTGLRSDVVTLYQGGKYNLYRYKKYTDVRLVFAPEFQAAFFGGDPDNFEYPRF
                     NIDMGLVRAYENNKPARVENYVRFSKDGSKEGDLVFVTGHPGSTQRLNTVAHVEALRD
                     TSIPLVIRMLERREAVLKRYMAMGEEQTRRAQNELNSTQNALKVYRGQLGGLKDPSLI
                     AYKQKSEATLRKAVMSNPQRQKEYGDAWDAIAKGRRDLAAYERDRRFLDLGAGFNTVL
                     FNYARTLVRLADESAKPNAERLPEFTDARRASLENVLFAQTNMYDDYEKMKLADSLDF
                     LREAYGADHELVKRILAGKTPEARAAELIDGSKLKDAAYRKQLAAGGKKAIDESTDPL
                     IQLARSIDKEARDLSRRYNNEVLGVERSAYAKIARALFETEGDKVYPDATFTLRLSYG
                     AVKGYTDEGKQITPYTTLGGLYERANQFGNKFPYVLPPRWMAKKSAVDPKTPFNVIST
                     NDIIGGNSGSPAINKKGELIGLVFDGNRQSIVGNFYYDEAVNRTISVDSRGMLEILRK
                     IFDATEIADELTK"
     gene            complement(7546..9858)
                     /locus_tag="VGB76_16355"
     CDS             complement(7546..9858)
                     /locus_tag="VGB76_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100519.1"
                     /translation="MRFKLFVVVLLALSLNVQAQTRTSRRERRSISNAAALVADAPAA
                     RVPEARDNSTRASVPGQTLPIRRVILYSNGVAYIERRGMVSGHAEVNLSFKQSQVDDV
                     LKSMIVLDLGQGRIGAVSYNSSAPPSARIADIPFSVASSTQSSGLAGVLGQLQGARVV
                     VTTANRTATGSILTVEERRSQIDADKPALVTHALVIASESGELSSFELADVRSVKLID
                     EGTRRDIAEFASATAAARRRDAKTIVVTSDGAGQREMLVSYTVAAPIWKTTYRVVLDA
                     AGKPFFQGWAIVDNVSEEDWNGVQLSLISGTPVSFIQPIQQPFYRYRPVIPMPGNLSL
                     NPQVHDPLEIDGMSANGMAAMVPPTAAADYAAPPPPPSPQDKLKASVRGRGARASAEQ
                     RQDLLLRQQNQVNNLDLLAEGTSLATGQGSLSAAITGEGSGIAAAAEGSEVGDLFEYQ
                     IAQPVTVKRDRSALIPILQTRMEGARVSIFNEAVRRERPMSGMLLKNTSTLTLEDGAM
                     TVIDGNAYAGEALMERLKPAEQRLISFALDLGTLVNVRMKEDRAPAYLVRILNGVFQA
                     HYHRQDKKTYSLANQTDHTRILYVEHPVRPGWELNDKETPKPEGRSARYYRFRVELKP
                     HERTELTVTERQPLVDTFALSNVTTDQLGLFVSRSYIDDKTRLALQNIVEIKSRIAAI
                     DARLQAIDTEITEIGEDQTRLRENIETLAQTAEAKQLIARYVAKADQQETRIEQLTKE
                     RQAANEERGRLQAQLDAAIRALSFDRNLTE"
     gene            complement(10167..12674)
                     /locus_tag="VGB76_16360"
     CDS             complement(10167..12674)
                     /locus_tag="VGB76_16360"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEY0100520.1"
                     /translation="MKFPRSVRSSGASLLVAVSLLLSSIVSAQRPARNPPAPPAPRTP
                     SDTTAAANEKTPAATFDTLLSADAYGVYAELRMVGRQAGSQELAQLLTPLTLSGVGGA
                     PDELLNFYEFLKTHAEPLMTARLMFAAMPVRAGLPESLVAVEMPSVEEAQKFLPEVKQ
                     FIAVNFAPQPRVADTAVVADASTRTEDTTVTNNRRGRRRARERGAKGNARAEAAKPPA
                     APPVQIKRSGSIIAMSGESFTFKNLRGGRDQALLVNEPGFQAARTRFGTDSLFIYFNT
                     TRMSSSSKLRMEAYEKEHQRQVELARVESEKQVAASNLNMSRGSMGDGNMSGNTNNAN
                     TNAPPSIEDEPPAPLETPEPTPSPKSEKELEEERQREQSRQFAQTVGSLVFGDGPRGN
                     STSAWPESIGVGVSFEADALVARALFISLTDDQPLRPVPFLPVVLSGPSIATEAPGVL
                     PAETDIFLTMSLDLPQMYDYLASMMKILDLAATASGEGKGGLAEQLTSFEKTNNFRIK
                     EELLAALGNEIAIGLPSQFLGVRSGKTKSADDASDAPALSGPIAVIALNDKKALQELL
                     PRVLASVGFVGATEQSIIEKHGEVEVLNFSGGTLAFIDRFLVSAPDAAAMHRIIDAYN
                     SGETLANNQRFRDSTGWQARQAIGQAYVSNTLLKSMFEDVSKSVADIDEEAMRAYLLQ
                     LDPDPGSITLSATRESDGLMHELHLPKNFLSLLSASSVIGQKLSTLRSNESTAQWKLR
                     MIHGAQGEYKVGKGRYGTLEELKAAGQLNEEHQTLEMEGYEIKLSVSGDGFEATATPT
                     GYPKQGRRSFYIDAAGELRGGDKGGKLASVSDPIIDF"
     gene            complement(12937..15306)
                     /locus_tag="VGB76_16365"
     CDS             complement(12937..15306)
                     /locus_tag="VGB76_16365"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0100521.1"
                     /translation="MDFLSGLNEQQRIAVTTTEGPLLILAGAGSGKTRVIVHRIAYLI
                     AERGIAPYHILAVTFTNKAAGEMRERVERLLSEQRLQSAPLISTFHSLCVRILRRDIE
                     KLEEGHTRSFTIYDQDDSTRIVKAAIKDLGLDSDRMGARQVQSAISHAKNSGTDAEAY
                     AARVEYGDEKRAAIARVYRLYEERLVNNNALDFDDLLIKAVRLLRKNREVRDQYNDKF
                     RYILVDEYQDTNSLQFALIRFLTEKQQNVCVVGDEDQSIYKWRGADISNILNFEQHYP
                     EAKAIRLEQNYRSTQNILDTAGAVVKLNTERKGKTLWTSNPAGERIRYYQAMDAEGEA
                     RFVVAKVEEHRRVDPSLRAAVLYRTNAQSRVFEEAMRRANIAYNIVGGFSFYERAEVR
                     DIISYLKLAMNPHDSIALMRVINTPPRGLGKQTLDELDRRAKDYGVSLWETLAIVTDP
                     QQTTSGFAARAASSLKNFQRIITNLVSVAQREPASEVVKAAILDSGYADALKSENTEE
                     AEGRLENLQELVNAAVDYDREEGGGLRDFIDAAALVSDTDQYKRDAPVTLMTVHSAKG
                     LEFPLVFMVGLEDGLFPHSRSATDRAELEEERRLCYVAITRAERFLYVTHAMKRRVYG
                     EELASEPSQFLNEMPFDLMEDLSRGASWLSFARSSERLENAHAARALRGETRPTEKPY
                     AGKTYNSADSIAEFFRQRGQQVNPQSAPPQPAIKRRTEVGGKSSSANASSSVEAGGFA
                     PGTHVKHAKYGRGLVLRREGTGDAAKLTVSFPGFGQKKLIEKYAGLEKA"
     gene            complement(15398..15955)
                     /locus_tag="VGB76_16370"
     CDS             complement(15398..15955)
                     /locus_tag="VGB76_16370"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEY0100522.1"
                     /translation="MRHENTLDAAQTALVIIDMQEAFRTSISDFAETSARIALMAHAA
                     QLLNVPIVVTEQYPKGLGRTAGEIRAVLPDALEPIEKTAFSSCGADEFVGALERTGAR
                     QVLICGIEAHVCVNQTTHDLLARGYKVHLLTECITARSAHNRQTGLLKMRQSGALPSS
                     TEMSLFELMRDAAHEQFKAVQKLIK"
     gene            16289..>16566
                     /locus_tag="VGB76_16375"
     CDS             16289..>16566
                     /locus_tag="VGB76_16375"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100523.1"
                     /translation="MRKRILMLALMAAISLGGMEIMAQTTAQPPVAKRATKKQAFHGE
                     SLDDDYFWLREKTDKEVVAYLEAENAYTEAMMKGSEAFQEKLYQEMLGR"
BASE COUNT         3152 a         4911 c         5201 g         3302 t
ORIGIN      
        1 ccgactcgcc gctcgccgaa gccaagttct cgcaagacca cctgaccgtc ctgacgacgc
       61 ttgcctcggt ggccgccatc cgcgtcgaaa acgcgcgcct gatggaagag gatttggagc
      121 gcgaacgctt cgagcgcgaa ctcaatctgg cgcgcgagat tcagcaacgc ttccagccga
      181 gcggcgcgcc gaacgtcaac ggctacgaga tgcagggcat cagtttcccc tgctacgaga
      241 tcggcggcga ctattacgat ttcatccagc gacccgacgg gcgcatggtc atcgcgctcg
      301 gcgacgtgtc gggcaaaggc acgtccgccg cgctcctcat gtcgagcctg cacgcggcga
      361 tgcacgcgca gattcccgcg caccagacgc tcggcgaaac catctccgcc gtcaaccgtt
      421 atctcgcgga gacgatcccg ctcaaccgtt tcatcacgct cttctgcgcc gaactcgacc
      481 ccgcgaccgg cacgctctcc ttcctgaacg cagggcacaa cccgccgctc atcgcgcacg
      541 ccggaggctc gatggagcaa ctcgctgcgg gcggcatccc tctcggcatc gtggccgacg
      601 ccgtctaccg cgaaggccgg acgcaactcc aacccggcga cgcgctcgtc atctattccg
      661 acggcgtctc cgagacgcag aacgcggcgg gcgaagagtt tggtcccgtg cgtctctaca
      721 acacggtcgc gcgctatctg gactccaccg ccgcgggcat ccgcgacaag atcgaagccg
      781 acctcaccaa gtttgcgcag ggcacgcccg ccggagacga catcacgctc gtcatcgtca
      841 aacggcaggc cgaaggcgcg atggtcgcat cgagccggag ctaaacgcat tcgagtctca
      901 tccggttgac gggtgaaaaa ggacgtgagc agcatgactt cgacagccca actccggcgg
      961 cgtttcctaa cccggttcaa gtgctcattc atcgcggcgt tcgtgtgcct ttcgcttgcg
     1021 cctgccgctg ccgcgcagca aaaggcgcgt gcgcgcgatc tgggcatccc gttcgaaggc
     1081 aggccgggga cgcacaacgc gatcacggac gtggcgggcg tcgaagtcgg gcacacgact
     1141 ttgatctccg ggagcggtcg gctggtgaga gggcaggggc ctgtacgcac gggcgtcacg
     1201 gcggtcttgc cgcgcgggaa aaaatctttc gaccccgtct ttgccgcatg gtttcccttg
     1261 aacggcaacg gcgagatgac cggcacgacg tggattcggg agtcggggtt tctcgaagga
     1321 ccaatcctca tcacgaacac gcacagcgtc ggcgtcgtgc gcgatgcgac gattgactgg
     1381 gagatcaggc acgggcgcaa gtttctcttc acctacccga ttgtagcgga gacttatgac
     1441 ggcgtgttga acgacatcaa cggctttcac gtcaaacccg aacacgtgtt ctccgcgctc
     1501 gacgggtcga gcggcgggcg cgtggccgaa ggcaacgtcg gcggcgggac ggggatgctc
     1561 tgcaacgggt tcaagggcgg catcggcacc tcttcgcgca tcgtcgcggc ggaagtcggc
     1621 ggctacacgg ttggcgtgtt ggtgcagtgc aattacggca gccgtcgcgg cctgcgcatc
     1681 gcgggcgtgc cggtggggca ggagttcacc gatccgtcga tctgcttgga agggatggat
     1741 gcaccttcgc aggagttcct gcgcggtatg ccgcgctgcg gaaagtctgc ggcctccctt
     1801 gagcgtccgc accccgaact cggttcgatc atcatcgtcg tcgccacgga cgccccgctg
     1861 cttccgcacc aactcgaccg cctcgtcaag cgcgccgcgc tcggactcgg acgcgaaggc
     1921 tccatcgcca gcaattcctc cggcgacatc ttcgtggcct tctccacggc caacgccgga
     1981 gccgccgccg aacggggacc catcacgctc aagatgatgc cgaacgaaca actcgacccg
     2041 ctcttcgccg cgacgattca ggcgaccgaa gaggctgtca caaacgccat gctcgcggcg
     2101 gagacgatca cgggagccga caacgtgcgc gccgaagcca tcccgcacga acgcctgcgc
     2161 gagatcatgc ggaagtacaa ccgcctgccc gcgtcgcctt cgaatgcgaa gtaaattacg
     2221 tggagcgcga acttcttgga gtgtgcagga gaagcgtgag aggttgtgaa caaagagcga
     2281 cgctcgatca ttaaaacgct gtcgcgcgcc ctgcggctca agtgtccggc ctgcggcctg
     2341 acttcaatcg tccaaagccc cttccacatc aagcatcaat gctctgcgtg cgacgtgttg
     2401 ttcaaacgcg aggccggatt tttcgtcggc gcgatcatgg caaacgtggt cacgacggag
     2461 ttcgtgattc tcctgctcta cgtcgccgcg ctgcccgtca tcgacacgcg cttcgacctg
     2521 gttctgacct gcctctccgt cgtcgcgctc atcttccccg tcgctttcta ccatcacagt
     2581 tggagcctct ggctcggctt cgaccacctc atcgaaactt tgccgaaggc tcctaaagct
     2641 caaagctgag agggcgggtc ttgaagattg acgggcgaga cgatgagatg agatttgcct
     2701 tctttcacag aacggagtcg agcatgaaat taagccgcat acttttcctg ttgcttctgg
     2761 gctgcatcct ttcggaaata gagacgggcg cgcaggcgac gaaacccgtg cgcccacccg
     2821 gcagaggggg tatgtggaag agccgtcttg gttgcgcgcc ccgaaaactc tttcgcggcg
     2881 attctttgcg actcacaatg tccgtgccac acggcggcga tttagccatc gtcgctcccg
     2941 gcgacggata cttcttactc agcttctggc agcccgataa aacagacgcg cgccaatctc
     3001 tcttcgattg ggaggcgttc aaaagcaggc gacaactgac gcttgatacc gcgcgactta
     3061 aagccaagcc ctgggtcgcg ggcagagatg aacctgagcc ggtctttagc cggacgggct
     3121 ggtatcgagt gcgcctgagt gagaatttgg agactgacga cgggacgccg gtttccgaat
     3181 gtcgagtcta ttatgtcaac gcaaagaggc gttgacggca gccgtcgcaa gtcggataag
     3241 tgcgtggcgt gatctctaac ccgcgtcgta attcttccgg ctcaaactgt cggccaacgt
     3301 tagactccac acctccggct gcctctctct gtttcccgcc gccccgcctt cacccgcgac
     3361 tacgttccag catgatcagc atcaggaggc cgagcagggc gcgcgtctct tcggcttgat
     3421 cgagttcggc gcgcttctcg atgcggaact tgccctcgaa gaaggcgggt tgtttctcca
     3481 tgcggagcag caccgtgccg tcggcgcgcg agagtagata ggccgggtgg aagagataac
     3541 cggagagtgc gccgatgatc ggcaactggc cgatcacgct gtcgaacact ttgatccacg
     3601 cgttctcttc cgtgaccgac accgcgccgc cgggggcgtc gtcgtcaatt tcgtaatgcg
     3661 acttccagag cgagcgcatc ccgtgtcgtt tgatcgagcc gagggaattg cccgcgttgt
     3721 ctgtaaagcg gtagcgtgcg gagaagtcca tcacgcggtc ggcgttgatg tgatagaggg
     3781 gacgttgctg cgcctcgtcg gcgaagactg tgagcgcctc tttcaacttg aacgcttttc
     3841 gtttgacgta aaagatcaag ttgccttgcg cgtccctgac ggagagttgt tgcgcgaggg
     3901 cgatgagttt gaaagagatg tcgagcgggt agttcatgcg tgtctccaga agcgtgtgcc
     3961 ggagcggcaa cggattgttg aattgtttgg cggacggacg attcgacgcg atcccgttca
     4021 cctcaggaac ggactcatga caagcaataa tcccacggcg gcgagcaacg cgaggacggt
     4081 tgaaacgatg ccgagcgcgc gggagcgcac ccagccgctc agccccaaac tcgccagaaa
     4141 gaggaacatg catatgatga acaggagcga taagcggctg tgcatcaggt taaactttgc
     4201 tgctgcctgg agcgacgcct ccgcgccgcc gttcgcgggc gcagacatta ctatcactga
     4261 agccatcagc gtccacccga tgacacagac gatcaacccg gctgccgtcg ccatcgcagt
     4321 cccggctgtc cattcgacga tacggctccg cgaaggagcc gtcaggagca cgccgaggaa
     4381 ggcgaaggca aagcccccga tgagcgcgcc gacgaaggcc agttgacgaa acagatcggc
     4441 cagagattcg ggtgtggtat tcatggagat tttcagctta aggtgaacag cggctcaacg
     4501 cctgacaggc ttccgggtgc cggattacta cgcgattgag tagatcatta acgctgtcgc
     4561 cggactggcc cggtttagat tcccctctgc ctgctgctac ttcactttca aactcgcggc
     4621 gatgcgctca gccgtcttct cgccgatgcc gtcaaccatc tccagcccgc cgcccgcgac
     4681 gaacttccgt agatcgtcga gcgaagagat tttaaactgc tggtgcaggg cggcggcggt
     4741 cttgatgcca atgccggaaa cgtcgagcag gtcgagcacc gtctcgggcg tctcggcgat
     4801 gagcttctcg taagcctcga acgtgccgcg ttcgcacagt tcgacaatct tgccgctgat
     4861 ggcgcgacct acggagggga tcgcctgcaa gcctttcgcg ccctcctcgc gggcaatttg
     4921 cgcaaggggc gtcggccacg tctcgatgat ctcggaggcg ttgcgatagg agcgcacgcg
     4981 ataacggtcg tcgccgcgta tctccatgat gagcgcgagc cgctcgaagc ggcgggcgat
     5041 ctctccgttc tccataaaac agctccgggg gaagggggaa gggttaaagg ggaaggcggg
     5101 atacgcgtcg gggcgcgacg gtttacatgg cttttaccct tcccctctgc cccttccccc
     5161 tttcccaatg tcttctactc ggaggcatcc cggcgcggct tatgatactc taaatgagtt
     5221 cactcccatc aactttaacc tttttccgag gagctatatg aatattagaa aactcgcgcg
     5281 cactttgctc gccgcgctca tcaccttctc gtcgtttgcc ggtttcacga cggcgcgcgc
     5341 cgacgagggc atgtggcctt tcaatcaagt gccgagagcg gagattaaac gcaaatacgg
     5401 atttgaagtt accgatgaat tcctgaagaa actgcaactc gcctcggtgc gcttccccaa
     5461 cgggtcgggt tctttcgtct cgcccgacgg tctggtctta accaattatc acatcatcga
     5521 agatgccgtg ggcgaactcg gttcggcgga aaaagattac gccaaggaag gcttcgtcgc
     5581 acgcacgcgc gccgaggaac tcaagattcc cggctacgaa ctgaacgtcc tccagagcat
     5641 cgaagacgtg acggatcgcg ttaacgcttc ggtcacgcct gcgatgtccg ccggagacgc
     5701 tttcaacgcg cgccgcacgg ccattgccgc catcgaaaaa gagtcgaccg agaagaccgg
     5761 actgcgcagc gacgtggtca cgctctatca gggcggcaag tacaatctct atcgctacaa
     5821 aaaatatacg gacgtgcgcc tcgtcttcgc gcccgaattt caggccgcct tcttcggcgg
     5881 cgacccggac aacttcgagt acccgcgctt caacatcgac atgggactcg tccgcgctta
     5941 cgaaaacaac aagcccgcgc gcgtcgagaa ctatgtgcgc ttctcgaagg acggaagcaa
     6001 ggaaggcgat ctcgtcttcg tcaccggcca tcccggttcg acgcaacgcc tgaacacagt
     6061 ggcgcacgtc gaagccctgc gcgacacgag catcccgctc gtcatccgca tgctcgaacg
     6121 ccgtgaagcg gtcttgaagc gatacatggc gatgggcgaa gagcagacgc gccgcgcgca
     6181 gaacgaactc aacagcacgc agaacgcgct caaggtttat cgcgggcaac tcggcggctt
     6241 gaaagacccc tcgctcatcg cgtacaagca gaaatccgaa gcgactctgc gcaaggccgt
     6301 gatgtcgaac ccgcagcgac agaaagagta tggcgatgcg tgggacgcga ttgccaaagg
     6361 tcgccgcgat ctggccgcat acgaacgcga ccgtcgcttc ctcgatctcg gcgcgggctt
     6421 caacacagtc ctcttcaact acgcgcgcac gctcgtgcgt ctcgcggacg agagcgcgaa
     6481 gccgaacgcc gaacgcctgc ccgaatttac ggatgcgcgc cgcgcctcgc tcgaaaacgt
     6541 gctcttcgcg cagacgaaca tgtacgacga ttatgaaaag atgaaactcg ccgattcgct
     6601 cgatttcctg cgcgaggctt acggcgcgga tcacgaactc gtcaagcgaa ttctcgcggg
     6661 caagacgccg gaagcgcgcg ccgccgaact catcgacggc tcgaagttga aggacgcggc
     6721 ttatcgcaag caactcgcgg cgggcggcaa aaaggcgatt gacgaatcga ccgacccgtt
     6781 gatccaactc gcacgcagca tagataaaga agcgcgcgac ctgagccgtc gttacaacaa
     6841 cgaagtgctc ggcgtcgaac gctcggccta cgccaagatc gcgcgtgcgc tcttcgaaac
     6901 cgagggcgat aaggtctacc cggatgccac tttcaccctg cgtctctctt acggcgcggt
     6961 gaaaggctac acggacgagg gcaaacagat cacgccttac acgacgctcg gcggactcta
     7021 cgaacgcgcc aaccagttcg gcaacaagtt cccgtatgtc ctgccgccgc gctggatggc
     7081 gaagaagtct gcggttgatc cgaagacgcc cttcaacgtc atctcgacga acgacatcat
     7141 cggcggcaac tccggctcgc ccgccatcaa caagaagggc gaactgatcg gcctcgtctt
     7201 cgacggcaac cgacagtcca tcgtcggcaa cttctactac gacgaagcgg tcaaccgcac
     7261 gatctccgtc gattcgcgcg gcatgctcga aatcctccgc aagattttcg acgccacaga
     7321 gatcgcggat gaattgacga agtaaaccgg gccagcttcg agccttgatt aacaaagagg
     7381 acggtcggag ggtaatccct cagccgtcct ctttttttgg tgcggttaaa ccttcgagtg
     7441 ctcaccacag aggcatcttc tctgtgccct ctttatgtcc tctgtgcctc tgtggtgaag
     7501 gcttccttct tattcatccc tcatccctca tccttcatcc ctcgattatt ccgtcaggtt
     7561 gcggtcgaag gagagggcgc ggatggctgc gtcgagttgg gcttggagac gcccgcgttc
     7621 ctcgttggcg gcttgtcgct cctttgtgag ttgttcgatg cgcgtctcct gctggtcggc
     7681 tttggcgacg taacgcgcga tgagttgttt ggcttcggcg gtctgtgcga gcgtttcgat
     7741 gttttcgcgc aggcgggtct ggtcttcgcc gatctccgtg atctcggtgt ctatcgcctg
     7801 gaggcgtgcg tcgatggcgg cgatgcggct cttgatctcg acgatgtttt ggagcgcgag
     7861 gcgcgtcttg tcgtctatgt agctgcgcga gacgaagagg ccgagttggt cggtcgtgac
     7921 gtttgagagc gcgaaagtgt ccactagcgg ctgtcgttcg gtgacggtca actcggtgcg
     7981 ctcgtgcggc ttgagttcaa cgcggaaacg atagtagcgc gcgctgcgcc cttcgggctt
     8041 cggcgtctcc ttgtcgttca actcccaacc cggcctgacc ggatgctcga catagaggat
     8101 gcgcgtgtgg tcggtctggt tggcgagcga gtaggtcttc ttgtcctgcc ggtgatagtg
     8161 ggcttgaaag acgccgttca ggatgcgtac gaggtaggcg ggcgcgcgat cctctttcat
     8221 gcggacgttg acgagcgtgc cgagatcgag cgcgaaggag ataagccgct gctctgcggg
     8281 cttgagtcgt tccatgagag cttcgcccgc gtaggcgttg ccgtcgatca cggtcatcgc
     8341 gccgtcttcg agcgtgagcg tggaagtgtt tttgaggagc atgccgctca tggggcgttc
     8401 gcggcgcacg gcctcgttga agatggagac gcgcgcgcct tccatgcgcg tctgcaagat
     8461 cgggatgagc gcgctgcggt cgcgcttgac ggtgacgggc tgcgcgatct ggtactcgaa
     8521 gaggtcgccc acttcgctgc cctcggctgc cgccgcgatg ccggagcctt cgcccgtgat
     8581 ggccgcgctc agagagcctt gcccggtggc gagggaagtg ccttcggcga ggaggtcgag
     8641 gttgttcacc tgattctgct ggcggagcag caagtcctgt ctctgttcgg cactggcgcg
     8701 cgcgccacgc ccgcgcacgg acgccttcaa tttatcctgc ggcgaggggg gcgggggagg
     8761 cgcggcataa tccgccgccg ccgttggggg aaccatcgcc gccatgccgt tcgcgctcat
     8821 gccgtcgatc tccaacgggt cgtggacttg cggattaaga ctcaggttgc cgggcatggg
     8881 gatgaccgga cggtaacggt agaagggctg ctggatcggc tggatgaagg agacgggcgt
     8941 gccggagatg agcgagagtt ggacgccgtt ccaatcctct tcgctcacgt tgtcaacaat
     9001 cgcccagccc tgaaagaagg gcttgcccgc cgcatccagc acgacgcggt aggtcgtctt
     9061 ccagatgggc gcggcgaccg tgtagctgac gagcatctcg cgctgtcccg ctccgtccga
     9121 ggtgaccacg atggtcttcg catcgcggcg acgcgcggcg gcggtagcag acgcaaactc
     9181 cgcgatgtcg cgccgcgtgc cttcgtcaat caacttcacc gaacgcacgt ccgcgagttc
     9241 gaagctcgat agctcgccgc tctccgaggc gatgacgagc gcgtgcgtga cgagcgcggg
     9301 tttgtccgcg tcaatctgcg aacggcgttc ttcgaccgtg aggatcgagc cggtggcggt
     9361 gcggttcgcc gtggtgacga cgacgcgcgc gccttgcagt tggccgagca ccccggccag
     9421 cccgctgctc tgcgtcgagg aggcgacgga gaagggaatg tcggcgatgc gcgccgaagg
     9481 cggagccgac gagttgtagc taaccgcgcc gatgcgcccc tgtccgagat cgagcacgat
     9541 catggacttc aacacgtcgt ccacctgcga ctgtttgaaa gagagattga cttcggcgtg
     9601 cccggacacc atgccgcgcc gctcgatgta ggcgacgccg ttgctataga gaataacgcg
     9661 gcgaatgggc agggtttgcc ccggcacgct cgcgcgcgtt gaattgtcgc gcgcctccgg
     9721 tacgcgcgcg gcgggcgcgt cggcgacgag tgccgccgcg ttcgagatgc tgcggcgttc
     9781 gcggcgcgag gtgcgggttt gcgcctgcac gttgagagag agggcgagca ggacgacgac
     9841 gaaaagtttg aatctcatat cagcctccga gataaattgt ggctttgcgg ccagtgctaa
     9901 aggaaagaac gggcgaagta ggtttgcttg cgtctcagag agacgaatta agagaagaat
     9961 cgcagcggaa aattactggc gtgcgtgtct gacgtgcgtg tttgtgcgcg agacgaagga
    10021 tgaaacagcc cgagccggaa gagatgacgc agaatacagg acacagaata caggagtcag
    10081 aagagaagca agcgtctttc cttctgactt ctgtattctg actcctgtgt tctgtgttct
    10141 acggattcat tcttcattcc ggtcttttag aaatcaataa tcgggtctga gacggaagcg
    10201 agtttgccgc ccttgtcgcc gccgcgtaat tcgcccgcag cgtctatgta gaaggagcgg
    10261 cgtccctgtt tggggtagcc tgtgggcgta gccgtcgctt cgaacccgtc gccggagacg
    10321 gacaacttga tctcgtagcc ctccatttcc aaagtctggt gctcttcgtt gagctggcct
    10381 gcggctttga gttcttcgag cgtgccgtag cgtcctttgc ccactttgta ctcgccctgt
    10441 gcgccatgga tcatgcgcag tttccactgc gccgtcgatt cgttgctgcg cagcgtcgag
    10501 agtttctggc cgatgacgct gctggccgac aggagcgaga gaaagttttt cggcaggtga
    10561 agctcgtgca tgaggccgtc ggactcgcgc gtggcggaga gcgtgatcga gccggggtcg
    10621 gggtcgagtt ggaggagata ggcgcgcatg gcctcctcgt ctatgtcggc gacggatttg
    10681 gagacatcct caaacatgct cttcaggagc gtgttcgaga cataggcttg accgatggcc
    10741 tgtctggctt gccagccggt cgagtcgcgg aaacgctggt tgttggcgag cgtctcgccg
    10801 ctgttgtagg cgtcgatgat gcggtgcatg gcggctgcgt cgggcgcgct gacgaggaag
    10861 cggtcgatga aggcaagcgt gccgccggag aagttgagca cttccacttc gccgtgcttc
    10921 tcgatgatgc tctgctcggt cgcgcccacg aagccgaccg atgcgaggac gcgcggcaga
    10981 agttcctgca aagctttttt gtcgttcaga gcgatgactg cgatggggcc ggagagcgcg
    11041 ggcgcgtcgg aagcgtcatc cgcgctcttc gttttgccgg aacgcacgcc gaggaattgg
    11101 gaaggcaggc cgatggcgat ctcgttgccg agcgcggcga gcagttcttc cttgatgcga
    11161 aagttgttcg tcttttcaaa ggaggtgagt tgctccgcca acccgccttt accttcgcca
    11221 ctcgcggtgg cggcgaggtc gagaattttc atcatcgagg cgagatagtc gtacatctgc
    11281 ggcaggtcga gcgacatggt gaggaagatg tccgtctcgg cgggcaacac gcccggcgct
    11341 tcggttgcaa tggaaggacc agagagcacc accggcaaaa aggggacggg gcgcaagggc
    11401 tgatcatccg tcaggctgat gaagagggcg cgggcgacga gtgcgtcggc ttcaaacgac
    11461 acgccgacgc cgatggactc cggccatgcc gaagtgctgt tccccctcgg cccgtcgccg
    11521 aaaacgagcg acccgacggt ctgcgcgaac tggcgcgact gctcgcgctg gcgttcttcc
    11581 tccaactcct tttcgctctt cggcgaaggc gtcggctccg gcgtctcaag cggcgcgggc
    11641 ggctcatctt caatcgaagg cggggcgttt gtgttcgcgt tgttcgtgtt gccgctcatg
    11701 tttccgtcgc ccatactgcc gcggctcatg ttcaggtttg acgccgcgac ctgcttctcc
    11761 gattccaccc gcgccagttc cacctgtcgc tggtgctcct tctcgtacgc ttccatgcgc
    11821 aacttcgaac tgctcgacat gcgcgtggtg ttgaaataga tgaagagcga atcggtgccg
    11881 aagcgcgtgc gcgcggcctg aaagcccggt tcgttgacga gcaacgcctg atctcgcccg
    11941 ccgcgcaaat tcttgaacgt gaacgactcg ccgctcatgg cgatgatgct gcccgaacgt
    12001 ttaatctgga cgggcggcgc ggcgggcggc ttcgcggctt ccgcgcgcgc gttccccttt
    12061 gcgcctcgtt cccgcgcgcg ccgtctgcct ctgcggttgt tagtaaccgt cgtgtcttcc
    12121 gtccgcgtgc ttgcgtctgc gaccactgct gtatcggcga cgcgcggctg cggcgcgaag
    12181 ttgacggcga tgaactgttt cacctccggc agaaattttt gcgcttcctc aaccgacggc
    12241 atctcgacgg cgacgagact ttcgggcagc cccgcgcgca cgggcatcgc ggcgaacatc
    12301 aggcgcgccg tcatcagcgg ctcggcgtgc gtcttgagga actcgtaaaa attcagcaac
    12361 tcatccggcg caccaccaac gccgctgagc gtgagcggcg tgagcaactg tgcgagttcc
    12421 tgtgaaccgg cctgacgccc gaccatccgc agttccgcat agacgccgta agcgtccgcc
    12481 gagagcaaag tgtcgaaggt cgccgccgga gttttctcgt tcgcggcggc agtagtgtcg
    12541 gacggagtgc gcggagccgg gggggcgggc ggattccggg cgggacgctg cgcgctcacg
    12601 atggacgaca gcaggagtga gacggcgacc aggagagacg cgccgctgga acgcacagac
    12661 cgggggaact tcatgtctcg aatgtcctta ctctaaaaag gatagctcac ggatttcgcg
    12721 gagattggga gatcgccgga acgcagaaga ttatgacagg ggagagggaa aagataaatg
    12781 atgaaggcgg aacgatgaac gatgaaggag aagcagggtc ggaattcaga attcaggagt
    12841 cagaattcag gagtcagaat aaagcctgcg cgagtcttca tgctgactcc tgaattctgt
    12901 gttctccctc ctgcttcatc gttcatcatt ctcttctcac gctttctcaa gccccgcgta
    12961 tttttcgatc aacttctttt ggccgaagcc ggggaagctg actgtgagtt tggctgcgtc
    13021 gcccgtgcct tcgcggcgca ggacgaggcc gcgcccgtat ttcgcatgtt tgacgtgcgt
    13081 gcccggcgcg aagccgcccg cctcgacgga agacgacgcg ttggcggacg acgacttccc
    13141 gcccacctcc gtgcgacgtt tgatcgcagg ctgcggcggc gcgctctgcg ggttcacctg
    13201 ttgcccgcgc tggcggaaga actctgcgat agagtccgcg ctgttgtagg tcttgcccgc
    13261 gtagggtttc tcggtcgggc gcgtctcccc gcgcagcgcg cgtgcggcgt gcgcgttctc
    13321 cagccgttcc gaactgcgcg cgaacgagag ccacgatgcg ccgcgcgata agtcctccat
    13381 caagtcgaac ggcatctcgt tcaaaaactg cgatggttcg gacgccaact cttcgccgta
    13441 gacgcgccgc ttcatcgcat gcgtcacgta gagaaaccgc tccgcgcgcg tgatggcgac
    13501 gtagcacaga cgcctttctt cctccaactc cgcgcggtcg gtggccgaac gcgagtgtgg
    13561 aaagagtccg tcttcgaggc cgaccatgaa gacgagcgga aactccaagc ccttcgccga
    13621 gtggacggtc atcaacgtga cgggcgcatc gcgcttgtat tggtctgtgt cggagacgag
    13681 cgcggcggcg tctatgaagt cgcgcaggcc gccgccctct tcgcggtcgt aatccactgc
    13741 ggcgttgacg agttcctgca agttttccag acgcccctcg gcctcttccg tgttctccga
    13801 cttcaaagca tccgcgtaac ccgaatcgag aatcgccgcc ttgaccactt ccgaagctgg
    13861 ttcacgctgc gccaccgaga cgaggttcgt gatgatgcgt tgaaaattct tgagcgacga
    13921 agcggcacgc gcggcgaagc ccgatgtggt ctgctgcggg tcggtcacga tggcgagcgt
    13981 ctcccagagc gagacgccgt agtctttggc gcggcggtcg agttcgtcga gcgtctgctt
    14041 gcccagtccg cgcggcggcg tgttgatcac gcgcatgaga gcgatggagt cgtgcgggtt
    14101 catcgcgagc ttgagataag agatgatgtc gcgcacctct gcgcgctcat agaacgagaa
    14161 gccgccgacg atgttgtaag cgatgttggc gcgccgcatc gcttcttcaa agacgcgcga
    14221 ctgcgcgttc gtccggtaca gcaccgccgc gcgcagagag ggatcgacgc ggcgatgctc
    14281 ctcaacttta gcgacgacga agcgcgcctc gccctccgca tccatcgcct ggtaatagcg
    14341 tatgcgctcg cccgcggggt tcgaggtcca cagggtcttg cccttgcgct ccgtgttcaa
    14401 cttcacgacc gcgcccgccg tatcgaggat gttctgcgtc gaacgataat tctgttccag
    14461 ccggatcgct ttcgcctccg ggtagtgctg ctcaaagttc aggatgttcg aaatatccgc
    14521 gccgcgccat ttgtagattg attgatcttc gtcgcccacc acgcagacgt tctgttgttt
    14581 ctcggtgaga aagcgtatga gcgcgaattg gagactgttg gtgtcctgat attcatcgac
    14641 gaggatgtag cgaaacttat cgttgtattg atcgcgcacc tctctgtttt tgcgcaggag
    14701 gcggacggct ttgatcagga gatcgtcgaa gtcgagcgcg ttgttgttga cgagacgttc
    14761 ttcgtagagg cgatagacgc gtgcgatggc ggcgcgtttt tcgtcgccgt attcgacgcg
    14821 cgcggcgtag gcttcggcgt ccgtgccgct gttcttggcg tggctgatgg cggactgcac
    14881 ctgtcgcgcg cccatgcgat ccgagtcgag tcctaaatcc ttgatggcgg ctttgacgat
    14941 gcgcgtggag tcgtcctgat cgtagatggt gaaggagcgc gtgtgtccct cttcgagttt
    15001 ctcgatgtcg cggcgcagga tgcgcacgca cagggagtgg aaggtggaga tgagcggcgc
    15061 gctctgcaaa cgctgttcgc tcaggagccg ttcgacgcgc tcgcgcatct cgcccgccgc
    15121 cttgttcgtg aaggtgacgg cgagaatgtg atagggcgcg atgccgcgct cggcgatgag
    15181 ataggcgatg cggtgcacga tcacgcgcgt cttgcccgag cctgcgcccg cgagaatgag
    15241 cagaggcccc tcggtggtcg tgacggcgat acgttgttgt tcgttgagtc ctgaaagaaa
    15301 gtccatatca ataagtgacg agtgacaagt gacgagtgac gagcaagaat taaaacaagt
    15361 ctattgtttt aactcgtcac tcgtcactcg tcacttgtca cttaataagt ttctgcaccg
    15421 ccttgaattg ttcgtgggcg gcgtcgcgca tgagttcgaa gagcgacatt tcggtggaag
    15481 aggggagcgc gccggattgc ctcatcttga ggagtccggt ctgccggttg tgcgcggagc
    15541 gcgcggtgat acattcggtc aggagatgca ccttgtagcc gcgcgccagc agatcgtgcg
    15601 tcgtctggtt gacgcagacg tgcgcttcga tgccgcagat gagcacctgc cgcgcgcccg
    15661 tgcgttcgag cgcgccgaca aactcatccg cgccgcagga actaaaggcc gtcttctcaa
    15721 tcggctcaag cgcgtcgggg aggacggcgc gaatctctcc ggcggtgcgc cccaagccct
    15781 tcggatattg ttccgtgacg acgatgggaa cgttcagaag ttgggcggcg tgcgccatga
    15841 gggcgatgcg cgcggaggtt tcggcgaagt cggagatgga ggtgcgaaaa gcctcctgca
    15901 tgtcgatgat tacgagggcg gtctgcgccg cgtcgagcgt gttttcgtgt ctcatgtaga
    15961 aaatcttccc ggcaaccatt taagcacacg cggcggagaa agagaaaaag gggcaaggac
    16021 agtgacgagt gacgagaaag aaaagtgatg agtgatgagt gatgagtgat gagtgaaaga
    16081 cgaattttgg ttttaactcg tcacccgtca gttgtcactc gtgactcgtc gctcgtcgct
    16141 gttcttgccg tcttgggact tgagacttta taatcggggc acatccggcc tgatgcggtg
    16201 aagcccatat tcttcggaaa gttcaaacga gcaagccgaa cacttgcgcg ccccaactca
    16261 atacaacagc acagattgga gaagttcgat gagaaaacga atcttgatgc tcgcgctgat
    16321 ggcggcaatc tctctaggtg gaatggaaat tatggcacaa actacggcgc aacccccggt
    16381 cgcaaaaagg gctacgaaga agcaggcctt tcacggcgag agcctcgacg acgactattt
    16441 ctggctgcgc gaaaagaccg acaaggaagt ggtcgcttat ctggaagccg aaaacgctta
    16501 caccgaggcg atgatgaaag gctcggaagc ctttcaggag aagctctatc aggagatgct
    16561 cggacg
//
LOCUS       DASWNS010000476         7085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_407026, whole genome shotgun sequence.
ACCESSION   DASWNS010000476 DASWNS010000000
VERSION     DASWNS010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407026"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VGB76_16380"
     CDS             complement(<1..1357)
                     /locus_tag="VGB76_16380"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0100524.1"
                     /translation="MIIRCRQLKLTLVVLACLALLCLARGERVGSAQGGAGQPRELTA
                     QEKRGKQLYLKGESFEGAGDIRAFLGSDKLELLANTFACSNCHGLRGEGAREGGLQPP
                     PINWETLTAPHTSALTRRERPPYTEATLARAIKLGLDPTNAPLHPGMPQYDLKPEQMA
                     DLIAYLKKLGKESDADPGLDDKRIKVGAALPLTGALAQVGEDIKATLTAYFAALNAQG
                     GIYGRQLELVVVDSRGEPAATLEATQRLVEREGVFALVASFESSGSDATNDYLKRSNV
                     ALVGPVTLSPRLTVPPNPLVFYLLPTFGDQARALVDFAAAKFTERRARMAVISTDSEF
                     DADALAGVKAQAKLYPMEIVAEETSTRGSFPVATLVGMIAQKRPDGIFFFGDAEQFTQ
                     LTRAMEAAKLNNALFSSVVMIGHGAFNAPASIAPQIFLAYPAALPGQDDFTEFVNVMQ
                     KG"
     gene            complement(1488..2906)
                     /locus_tag="VGB76_16385"
     CDS             complement(1488..2906)
                     /locus_tag="VGB76_16385"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0100525.1"
                     /translation="MQLKFTRALFASIALVAFVALPEGSAVAQRKPAPKKAAVKKAVV
                     YSCPMDPEVKSKKPGRCPKCGMALRPLKEETPAEHEAHEKEGASTTSGTPSTSGATAN
                     GAATPIAPNSLKIPDTVVYNQHGQRLNFYTDLVKGKTVAINFIFTTCTTICPPLTATF
                     RKVQQELGERVGRDIELISVSVDPTTDVPERLKEFSSKFKVAPGWTFVTGSKPEVDGL
                     LKSLGAAVADKNDHTPMILIGNDAAGYWTRTYGLAPASTLVKVVNEAAAKSSATAAAA
                     AAAGSTSGDVQIPLPATKSATGAAVASPAGGTPRSAGGAAYFPNHILLTQDNQPVRFY
                     EDLLKNKIALINFMFTTCKGVCSPMTANLAKVQQQLGDRLGKEVVMLSFTVDHGTDTP
                     AVLKKYADSFKARPGWYFLTGKKENLDWVLYKLGGYVEDKNAHNLKIIVGNEATGEWM
                     KMHAMSNPNEITAAVLKMLPAK"
     gene            complement(2939..4903)
                     /locus_tag="VGB76_16390"
     CDS             complement(2939..4903)
                     /locus_tag="VGB76_16390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY0100526.1"
                     /translation="MWTSLMLLIVLVSAGNAQTAGPVSAPKKSDAAAPKNPATITQRF
                     EQQGIAVDFSLAAKPVEEGKPAALVAGADAIATFRVTDARTGQPVSGLHPNAWFSSRL
                     AAAAPTTETQCKDKIRTFMGGLLSARADLDLNSYLLVSLNHDRTLTFTNPQISFSKTK
                     LESIIPLPANGADFVLSKTKDRLYVTLPEKSAVLVIDTITRKIIETVNIEGQRKPTRI
                     ALEPDGSHVWVGLDDSPLVAVIDTTTNKLARTIGVGAGLHNIAFTADSRFAYVSNSAG
                     DTVSAIDTATLAKVADIAVGKTPVPVAFSTASGLVYVASLNGGSVTAIDPAKQSVAAT
                     IPLARGIVALGFEPRGRFAMVVNQLDSTVTVIDAATNKVIGSTSVVKSPDQVTFTNRY
                     AYLRGTASEKFSLVELDKLGREKVTAVDVQAGQRAPSTMPEELGVASMIVPTPEGNSV
                     MIAHAPDQMIYFYVEGMMAPMGTLSNYKRRPRGILLLDRSLTEIAPGTYATPIKLTRA
                     GSYDVPVLINENRIAHCFQADVGKSPNAPATEEKSSIVVAASFAGQEFKSGAGVPLKL
                     KLTDSITGQPIGGLGDVQVLVFEPPGVWQQRQWAKEVSAGEYEITQVFPHAGIFNVMV
                     RVGSRGVSFADLPFTPVIVKDGDVAGQKPE"
     gene            complement(5086..>7085)
                     /locus_tag="VGB76_16395"
     CDS             complement(5086..>7085)
                     /locus_tag="VGB76_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100527.1"
                     /translation="NLALADFALVYTRDLKPVNPPGRKVVGLPDIVAPPDIPQPESIS
                     VDDPGTSLINYRNEPIPLRVRDPNTNAQYADARGNMANVFRSKAENGTVIHGDPYTPL
                     LEVYEGDKIQVRIIQGAQEEQHVFNVHGSKWLHEPNVADSGYYNGQAIGISEHFEFEL
                     PPIPAVGNVKGANPDVADFMYSSAGTDNLWDGMWGIMREYKGLRPGLLALPGNSRGEV
                     STSDPGLRTDFCPSVSPKKTFYVEARLATDLQPGGITYNKEYGLVDPAGIVFIESGNV
                     AAVKAGTKKLEPLILRANAGDCINLQLTNLLPATLPDYPAWNFMPMLVEKFNLNNLKP
                     SNEVSLHPQLLEYDVRTSDGANIGLNDRQTVAPGEKITYRWYAGRVDIDPIDGSRHAT
                     PIEYGSVNLTDYGDIMKHGSHGAIGMLIIEPQGAKWSTAANSNAEALVYDPARSAFFK
                     EFAVVYQDDVIMKAPNGNPVRNYLAEEDSEDSGKKAFNYKTEPIWARLGLLNEMSKID
                     HNTFDHVEDLLNDMNQVNALSSLTHGDPETPIFTAAAGQQVRFRVVQPTGHPRQHAFT
                     VHGHAWYHEAWTDNSTRQWSPGVTPEPQSMTIGSQGGHTARRHWNIILPSAGGSFQQA
                     GDYLIRTQESYQFTDGMWGIFRVKPTCDTACMQQQQLLLSQ"
BASE COUNT         1194 a         2094 c         2256 g         1541 t
ORIGIN      
        1 cgcccttctg cataacgttg acgaactccg tgaagtcgtc ctgtccgggc aaggccgccg
       61 ggtaggcgag aaaaatctgc ggcgcgattg aagcgggcgc gttgaaagcg ccgtgcccga
      121 tcatcaccac cgaactgaag agcgcgttgt tgagtttcgc cgcttccatc gcgcgcgtca
      181 gttgcgtgaa ctgctctgcg tcgccgaaga agaagatgcc gtccggcctc ttttgcgcga
      241 tcatcccgac caatgtcgcc acgggaaaac tgccgcgcgt ggaagtctct tccgccacga
      301 tctccatcgg gtacagcttc gcctgcgcct tcacgcctgc gagcgcgtcc gcatcaaact
      361 cgctatcggt actgatgacg gccatgcgcg cccgacgctc cgtgaatttc gcggcggcga
      421 aatcgacgag cgcgcgcgcc tgatcgccga acgtcgggag caggtagaag accagagggt
      481 tcggcggaac ggtgaggcgc ggcgagagcg tgacgggacc gacgagcgca acgttgctgc
      541 gcttgagata atcgttcgta gcatcgctcc cgctcgactc gaaactggcg acgagcgcga
      601 acacgccttc gcgttccacc agacgttggg tggcttccag cgtcgccgca ggctcgccgc
      661 gcgaatccac gacgaccagt tcgagttgcc ttccgtagat gccgccctgc gcgttgagag
      721 cggcgaaata tgccgtgagc gtggctttaa tatcctcgcc cacctgtgcg agcgcgccgg
      781 tcagaggcaa cgccgcgcca actttgatcc gcttgtcgtc gaggccgggg tcggcgtccg
      841 attccttgcc gagttttttg agataggcga tgaggtcggc catctgttcc ggcttgagat
      901 cgtattgcgg catgccgggg tgaagcggcg cgttcgtggg gtcgaggccg agtttgatgg
      961 cgcgtgcgag ggtcgcttcg gtgtagggag gccgctcgcg tctcgtcagg gcggaggtgt
     1021 gcggggcggt gagcgtctcc cagttgatcg gcggaggctg gaggccgcct tcgcgcgcgc
     1081 cttcgccgcg caagccgtga cagttgctgc aagcgaaagt gtttgccagc agttccagtt
     1141 tgtcgctgcc gagaaacgcc ctgatgtcgc ccgcgccctc aaagctttcg cccttgagat
     1201 agagctgctt gccgcgtttc tcctgcgccg tcagttctcg cggctgaccc gcgcctccct
     1261 gcgcgctgcc gactctttcg cccctcgcca aacagaggag cgcgaggcag gcgagcacta
     1321 cgagagtcag tttcaactgg cgacaacgta tgatcatgtg cgctctttgt tctcgacttt
     1381 tgagggactg tggaggcgag gaacctgtca cccaaccgca ctccttccca gtcggcacgg
     1441 gcaggaacaa ggctcgcctc cacagttttt tttcgcggcc tgaactctta ttttgccggg
     1501 agcatcttca ggacggcggc ggtgatctcg ttcgggttcg acatcgcgtg catcttcatc
     1561 cattcgcccg tcgcttcgtt gccgacgata attttcagat tgtgcgcgtt tttatcttcc
     1621 acgtaaccgc ctagtttgta gagcacccaa tcgaggttct ccttcttgcc cgtgaggaaa
     1681 taccaacccg gacgggcttt gaaactgtcc gcatactttt tcaggacggc gggcgtgtcc
     1741 gtcccgtgat cgaccgtaaa cgagagcatc accacttctt tgccgagacg gtcgccgagt
     1801 tgttgttgca ccttggcgag attggcggtc atcggcgagc agacgccctt gcaggtggtg
     1861 aacatgaaat tgatcagggc gatcttgttc ttcaacaggt cttcgtaaaa acggacgggc
     1921 tgattatcct gcgtcaggag gatgtggttc gggaaatagg ccgccccgcc cgccgatctg
     1981 ggtgtgccgc ccgcaggcga cgcgacagcg gctcccgtcg cgctcttcgt cgcagggaga
     2041 ggaatctgca cgtcgccgct cgtgctgcct gctgcggctg cggcggcggc ggtcgcgctg
     2101 ctcttggcgg cggcttcgtt gaccactttg acgagcgtgg aagcgggcgc gaggccgtag
     2161 gtgcgcgtcc agtatccggc ggcgtcgttg ccgatcagga tcatcggcgt gtggtcgttt
     2221 ttgtcggcca ctgctgcgcc gagcgacttg agcagcccgt caacttccgg cttgctgccc
     2281 gtcacaaacg tccaacccgg cgcgacctta aacttcgaac tgaactcctt caacctttcg
     2341 ggcacgtccg tcgtcgggtc aacgctcacc gagatcaatt cgatgtcgcg cccgacgcgc
     2401 tcgccgagtt cctgctgaac cttgcggaag gtggccgtga gcggcgggca gatcgtcgtg
     2461 caggtggtga agatgaagtt gatggcgacg gtcttgccct tcaccagatc ggtgtagaaa
     2521 ttgagtctct gcccgtgctg gttatagacc accgtgtctg gaatcttgag cgagttcggg
     2581 gcgatgggag tcgccgcacc gttcgccgtc gcgccgctcg tcgaaggggt gccgctcgtc
     2641 gtggacgcgc cctccttctc gtgtgcttcg tgttcggcgg gcgtctcttc cttcaaaggt
     2701 ctgagcgcca tgccgcactt cgggcaacga cccggcttct tcgatttcac ttccgggtcc
     2761 atcgggcagg aataaacgac cgctttttta accgccgcct ttttcggggc gggcttgcgc
     2821 tgcgcgactg ccgagccttc gggcagcgcg acgaacgcga cgagggcgat ggacgcaaag
     2881 agcgcgcggg tgaattttaa ttgcatgttg atgaatcctt tcctcgcggc ggtagacctt
     2941 attcgggttt ctgcccggcc acgtctccat ctttgacaat gacgggggtg aacggtaaat
     3001 cggcgaagct cacgccgcgc gaaccgacgc gcaccatcac gttgaagatc cctgcatgcg
     3061 ggaagacctg cgtgatctca tactcgcccg cgcttacttc cttcgcccac tgtcgctgct
     3121 gccagacgcc gggcggctcg aagaccaaga cctgcacgtc gcccaacccg ccgatgggct
     3181 gacccgtgat cgaatcggtg agcttcaact tgagcggcac gcccgcgccc gatttgaact
     3241 cctgccccgc gaacgaagcc gccacgacga ttgaactctt ctcttccgtt gcgggcgcgt
     3301 tcggcgactt gcctacgtcg gcctgaaagc aatgcgcgat gcggttctcg ttgatgagga
     3361 cgggcacgtc gtagcttccg gcgcgcgtaa gtttgatggg cgtcgcgtag gtgccgggcg
     3421 cgatctcggt caggctgcgg tcgaggagga ggatgccgcg cgggcgtcgt ttgtaattgc
     3481 tgagcgtgcc catcggagcc atcatgcctt cgacgtagaa atagatcatc tggtcgggcg
     3541 cgtgcgcgat catcacggag ttgccttccg gcgtcggcac gatcatgctg gcgacgccga
     3601 gttcctcagg catcgtgctc ggcgcgcgtt gccccgcctg cacgtccacc gccgtcacct
     3661 tctctctgcc gagcttgtcg agttcgacca gcgaaaactt ctccgaggcc gtgccgcgca
     3721 ggtatgcgta tcggttggtg aaagtgactt gatccggact cttcacgacg ctcgttgagc
     3781 cgatgacttt gttggttgca gcatctatga cggtgaccgt gctgtcgagt tggttgacga
     3841 ccatagcgaa gcgaccgcgc ggttcgaagc cgagcgcgac gatgccacgc gcgagcggta
     3901 tcgtcgccgc cacgctctgt ttcgcggggt caatcgcggt cacgctcccg ccgttcagcg
     3961 aagcgacgta gaccagaccg ctggccgtgc tgaaagcgac gggcacggga gttttgccga
     4021 ccgcgatgtc agccactttg gcgagcgtcg ccgtgtcgat ggccgagacg gtatcacccg
     4081 cgctgttgga gacgtaggcg aagcggctgt cggcggtgaa ggctatgttg tggagtcctg
     4141 cgccgacgcc gatggtgcgt gccagtttgt tcgtcgtcgt gtcgatgacg gcgacgagcg
     4201 gcgagtcgtc gaggccgacc cagacgtggc tgccgtccgg ttcgagcgcg atgcgcgtcg
     4261 gcttgcgctg gccttcgatg ttcaccgtct cgatgatctt tctcgtgatc gtatcaatga
     4321 cgaggacggc ggatttttcc ggcagcgtaa cgtagagacg atccttcgtt ttgctcaaca
     4381 cgaagtccgc gccgttggcg ggaagcggaa tgatgctctc caacttcgtc ttgctgaatg
     4441 agatttgcgg gttggtgaag gtcagcgtgc ggtcgtggtt gagcgacacg aggagataac
     4501 tgttgaggtc gagatcggcg cgcgcgctga gcaggccgcc catgaaggtg cgaatcttat
     4561 ctttgcactg cgtctctgtg gtcggcgcgg cggcggcgag acgcgaactg aaccaggcgt
     4621 tcgggtgcag gccggagacg ggttgacccg tgcgcgcgtc ggtcacgcga aaggtggcaa
     4681 tcgcatcggc tccggcgacc agagcggcgg gcttgccctc ttcgacgggc ttcgccgcga
     4741 gcgaaaagtc cacggcgatg ccttgctgtt cgaatctctg tgtgatggtg gccggatttt
     4801 tgggggctgc cgcatcgctc ttcttcggcg cgctgacagg cccggcggtc tgcgcgttcc
     4861 ctgcggaaac caacacgatc aacagcatca agctcgtcca cattgatcgt cccgccggtc
     4921 tgcgaaatcc ttctctctta atttgataaa cggctggcat gataaagact tcctcttttg
     4981 ataagactta acttttatgg ttcgccatga ttatacggcg cgatatgcaa agattgaagg
     5041 gcaacctaac cgaagctagg ctgcccccca atgtgacgtg ggttactact gcgacaacag
     5101 cagttgctgt tgctgcatgc aggcggtatc gcacgtcggt ttgacgcgga agatacccca
     5161 cattccgtct gtgaactggt aactttcctg agttcgaatc aggtagtcgc cagcctgttg
     5221 gaaactgccg cccgcgctcg gcagaatgat gttccagtgg cggcgcgcgg tgtggccgcc
     5281 ctgcgagcca atggtcattg actgcggttc gggtgtgacg cccggcgacc attgccgggt
     5341 cgagttgtcc gtccatgctt cgtggtacca ggcatggccg tggacggtga aggcgtgctg
     5401 gcgcggatgc cccgtcggtt gaacgacgcg gaagcgcacc tgctgcccgg cagccgcagt
     5461 gaagatcggg gtttccggat caccgtgcgt cagcgaactg agcgcgttca cctgattcat
     5521 gtcgttgagc aaatcttcca cgtgatcgaa agtgttgtgg tcgatcttgc tcatctcgtt
     5581 gagaagcccg agccgcgccc agatgggttc ggtcttgtag ttgaacgcct tcttgccaga
     5641 gtcttccgaa tcctcctccg ccaggtaatt gcggacggga ttgccgttcg gagccttcat
     5701 gatcacatcg tcctgataga cgactgcgaa ctctttgaag aacgcgcttc tggccggatc
     5761 ataaaccaac gcttcggcgt tcgagttggc agccgttgac cacttcgcgc cctgaggctc
     5821 gatgatcagc atgccgattg cgccgtgcga accatgcttc atgatgtcgc cgtagtccgt
     5881 gagattgacc gagccgtact caatcggcgt ggcgtgacgt gagccgtcaa tcgggtcgat
     5941 gtcaaccctt ccggcgtacc agcgataggt gatcttctca cccggcgcga ccgtctgccg
     6001 gtcgttcaag ccgatgttcg cgccatcgct ggtgcgcaca tcgtactcca acaactgcgg
     6061 gtgcaggctg acctcgtttg aaggtttcag gttgttgagg ttgaattttt ccacgagcat
     6121 cggcatgaag ttccaggccg gataatcggg cagagtcgca ggcaacaggt tcgtgagttg
     6181 caagttgatg caatctccgg cgttagcccg caggatgagc ggctccagtt tcttcgtccc
     6241 ggccttcacc gccgcgacgt tgccgctttc gatgaacacg atgcccgccg gatcgacgag
     6301 gccgtactct ttgttgtagg tgataccacc gggctgcaag tcggtggcga gacgagcctc
     6361 cacgtagaag gtcttcttgg gcgagacgga cgggcagaag tccgtgcgca agcccggatc
     6421 gctggtgctc acttcgccgc gtgagttgcc gggcagagcg agaaggccgg ggcgcagccc
     6481 cttgtactcg cgcatgatgc cccacatgcc gtcccagagg ttgtccgttc cggcgctgct
     6541 gtacatgaaa tcagccacat cagggtttgc gcccttgacg ttgccgacag ccgggatcgg
     6601 cggcagttcg aactcgaagt gttcggagat gccgatggcc tgaccgttgt aatagccgga
     6661 atccgcgaca ttcggttcgt gcagccactt gctgccgtgt acgttgaaga cgtgctgttc
     6721 ttcctgcgcg ccctgaatga tgcgcacctg aatcttgtcg ccttcgtaga cttcgagcag
     6781 cggcgtatac gggtcgccgt gaatgaccgt gccgttctcg gctttcgatc tgaagacgtt
     6841 cgccatgttg ccgcgagcgt ctgcatattg cgcgttggtg ttcgggtcgc gcacgcgcag
     6901 agggatcggc tcgttgcgat agttgatgag gcttgtgccc gggtcgtcaa ccgagatgga
     6961 ctccggttgc gggatgtccg gcggtgcgac gatgtcgggc agcccgacaa ccttgcgtcc
     7021 cggcgggttc acaggcttca agtctctcgt gtagacgagc gcaaagtcgg ccagcgcgag
     7081 gttga
//
LOCUS       DASWNS010000477         6257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_277428, whole genome shotgun sequence.
ACCESSION   DASWNS010000477 DASWNS010000000
VERSION     DASWNS010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277428"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="VGB76_16400"
     CDS             complement(<1..110)
                     /locus_tag="VGB76_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY0100528.1"
                     /translation="MPRITGKVKWFNNAKGYGFIEQPGSSDIFVHYSAIQG"
     gene            complement(469..1380)
                     /locus_tag="VGB76_16405"
     CDS             complement(469..1380)
                     /locus_tag="VGB76_16405"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0100529.1"
                     /translation="MTRDDRLYAFIVARTSRSRSKIRRICIHKRWLKAASALACLVLC
                     AALYGLYGMFRDAQHINIERENTRLRVENEKQRQQLEKLENRVDAIEDTSRRIAEISG
                     TTTAPNEVESKQQGTSERGAGGPLVEMDAAAINAVETRAVQLEQELRAHESALRERAR
                     IPSIWPVAGGETSDSFGGRRDPFGGGSSEFHSGQDISAERGTPVYAAGIATVKFAGTQ
                     SGYGQIVILDHGDGITTRYGHLSKIEVEAGQQLARGTIIGRVGSTGRSTGPHLHYEVR
                     INEEPVNPRAYLPAPATVSGGRTCAQP"
     gene            complement(1747..1962)
                     /locus_tag="VGB76_16410"
     CDS             complement(1747..1962)
                     /locus_tag="VGB76_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100530.1"
                     /translation="MTALLATFGRWGGILTLIALLIVLVKQLIALVGFLMFALKIAIV
                     IAFVGVLLLIVLTMLRARGQRRREAGE"
     gene            complement(2114..2893)
                     /locus_tag="VGB76_16415"
     CDS             complement(2114..2893)
                     /locus_tag="VGB76_16415"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEY0100531.1"
                     /translation="MNCSYHNMNASVVQCSRCARPLCPACDHRIRGFPYCQDCIVAGV
                     ELLQQQTALLHAPQQLRRRTSKWVATLLSLLVPGLGAAYNGQTSKALVHFALFASCWQ
                     MAAVTNAVSFFVLGALGVWLFAAVDSYRTAQLISAGLTPNAESDPIARRLYGHPLAWA
                     IVLIALGSIFLLNTLFGVQFPVRQLLPVALVVLGAYMLFDFLRSRRERDMFDATGAPP
                     RSFMSSSSAPLDMTRFSTGELNSQLTGARRTDAPWPFDPRA"
     gene            complement(3006..3860)
                     /locus_tag="VGB76_16420"
     CDS             complement(3006..3860)
                     /locus_tag="VGB76_16420"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0100532.1"
                     /translation="MLCAEFEDRLTDYLEGSLDTTTQIAFNGHALRCPVCHDLLSEVK
                     NTLRACRTATVPAPSTSLDARILLHTAPESAMTCDEFEDYLTDYLDGFLPASLYHRWE
                     RHAALCDSCTNLPGEVVRSIGACYTYINEERPVPAALHARILQATLGTTEAEQLRAPL
                     SARLAELVRGWLDAIVSPQLATVATMLLVAVLVGTSTLSDDGSIGGMYRASLRFAART
                     YERGANLPVRRAVMSGDLKKVAGSLESLLGSPAQNEKETEQGQQQNAQPAANGSDASE
                     QQTGAQKR"
     gene            complement(3987..4592)
                     /locus_tag="VGB76_16425"
     CDS             complement(3987..4592)
                     /locus_tag="VGB76_16425"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0100533.1"
                     /translation="MLGAAKALTVNDGAELVRRCRAGDGAAWEEIVSLYSRRIYNLAY
                     RFTSRTDAAEDLTQEVFVRVYRSLEQYDPKQGDLQNWLMRLARNLVIDDYRKRQRAPQ
                     DSQGEDLEGHTYHLRAANSSPHVDMERRELGEQVQAGIDKLAPDLRTCVILRDIEELS
                     YQEIVDLLQIPEGTVKSRINRGRIELAKILRRMRVVAMSDV"
     gene            5048..6187
                     /locus_tag="VGB76_16430"
     CDS             5048..6187
                     /locus_tag="VGB76_16430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY0100534.1"
                     /translation="MRRYLKFIILTLVAAAVLWWFGRGLDWASVRAAIGKSDWRLIAL
                     AVLIVWGTYFVRALRWRALLAPLTEASLRELFAATTVGFSAVFLIGRAGEVVRPTFLP
                     LRDRRVRPAAAFVTIGVERIYDMVAVVVLFAVNLIWFRAPGGGDAAAYARVRQAGMLL
                     LVAAAVGIFALVVFKRYSRQVIDWCEAKFARAPPLLKRAGKLLTHLLEQVAGALSVLV
                     SARELAVTVGLTALLWGIIAVANWLVLRAFGLPFGATETLFVLGWALVGSLVPTPGGA
                     AGAFHAATAAGLIFLGIAREEAAAISIVLHLVVFAPAVVFGLYYFLRSDVSFERLRSL
                     TATEPHEDEEEERGDERDSTQAGRAAKDKAALSELSRDRSAGAET"
BASE COUNT         1220 a         1848 c         1909 g         1280 t
ORIGIN      
        1 ccctgaatgg cgctgtagtg aacgaagatg tctgaagagc cgggctgttc gatgaaaccg
       61 tagcccttcg cattgttgaa ccacttgacc ttaccggtaa ttctaggcat agctaacgaa
      121 tcctcctaac gattcggtga atcggtacaa aaacagtttt ctttgttctc gtgccggtgg
      181 gttaatactc tcatgagccg gaagcgtttg agcgaacgcc cccgtcagaa ctacatgaca
      241 acaaaaaagc ccgcacatgg cgaaccggcg tcaaagcgcg ttcttcacca catacggacc
      301 aagcgggaga ctaacaaact catttcggtc ttacttctag cactaaccgg atgaacgagt
      361 ttggctgtca actcgtcctt cgtaaaacga cggcgcatac taagcgaacc cgtcgagaac
      421 tgtcaagaaa aaaaataagc cggacacgcg cccttgatgc ctgcaacctt aaggctgcgc
      481 gcacgttctc ccgccgctca ccgtcgcagg cgcgggcagg taagcgcgcg ggttaacagg
      541 ttcctcgttg atgcgcactt cgtaatggag gtgcggtccg gtagagcgtc cggtggaccc
      601 gacgcgcccg atgatcgtgc cgcgcgcgag ttgctgcccg gcctcaactt cgatcttcga
      661 gagatgcccg tagcgcgtgg tgatgccgtc gccatgatcg aggatgacga tctgcccgta
      721 gccgctctgc gtgcccgcga atttcaccgt ggcgatgcct gcggcataga cgggcgtgcc
      781 gcgctcggct gagatgtcct gccccgaatg aaactccgat gagccgccgc cgaaaggatc
      841 gcgacgcccg ccgaaactat cactcgtctc gccgcctgcc accggccaga tcgaagggat
      901 gcgtgcgcgt tcgcgcaggg ctgattcgtg agcgcgaagt tcctgttcga gttggacggc
      961 gcgcgtctcg accgcattga tggccgccgc atccatttcg acgagcggac cacccgcgcc
     1021 gcgctcgctc gtgccttgtt gcttgctctc gacttcgttc ggcgcggtcg tcgtgccgga
     1081 aatttccgcg atgcgacgcg aagtatcttc gatagcgtca acgcggttct ctaacttttc
     1141 gagttgttgc cgttgctttt cgttctccac gcgcaggcgc gtgttctcgc gttcgatgtt
     1201 gatatgttgc gcgtcgcgaa acatgccgta gaggccgtag agcgcggcgc agaggacgag
     1261 acacgcgagc gcgctcgccg ccttgagcca gcgtttatga atgcagatgc gacgaatttt
     1321 agaacgagag cgcgaggtgc gagcgacgat aaaagcgtat aaacggtcgt ctctggtcat
     1381 cgggagaatg tgctccttta tcactaatct gcgcgtaaaa cacacaggga acagcgcaga
     1441 tgagcgcgaa aggcactgtc atccgaagcg ggcgacggtg cgcatgaaac cacaattggg
     1501 ggcttcttta ttaggtttga cctgcctgat tatggcagtt cggcagcttg atcagtatta
     1561 actcatccac gaggcgagcc gcttgatctg cttaaggggc agcaccctat cacaccccgc
     1621 agagtgtttg caaccgccga aacctgagaa aattctttaa ctaataacaa aaacccggtg
     1681 caaagttgcg tcctacaact ttgcaccggg tctgttttca aactcaaaaa acgaagcggc
     1741 tacgcgttac tctccggcct cacgacggcg ttgcccgcgt gcgcgcagca tggtcaggac
     1801 gatcagcaac aacacgccga cgaaagcgat gacgatggcg atcttgagcg cgaacatcag
     1861 gaagcccacg agggcgatga gttgtttgac cagtacgatc aggagcgcga tgagcgtcaa
     1921 gatgccgccc cagcgaccga atgttgcgag tagtgctgtc atgtaaaaat ctccctcgtg
     1981 ctaattattc ccgcgcctgc ttccggtaaa ctcacttgct cacttgtccg ccttgctcaa
     2041 gcgcgccttc atcataacaa agaacttcat cgcatggcga gacgcgcacg ccgcgtatcc
     2101 aaattcaaac tcttcaggca cgcggatcaa acggccaggg cgcatccgtg cggcgcgcac
     2161 ccgtcaattg cgaattgagt tcgccggtgc tgaaccgtgt catgtcgagc ggcgcgctgc
     2221 tgctgctcat aaaagatcgt ggcggcgcac ccgtcgcgtc gaacatgtca cgttcgcggc
     2281 gcgagcgcag gaaatcgaag agcatgtagg cgccgagcac gacgagcgcg accgggagca
     2341 gctggcgcac cggaaactgc acgccgaaga gcgtgttcag cagaaagatc gagccgagcg
     2401 cgatgagcac gatagcccac gcgagcggat ggccgtagag acgacgtgcg attggatcgg
     2461 attcggcgtt aggcgtcagt ccggcactga tgagttgcgc cgtgcggtag gaatcaaccg
     2521 ccgcgaacaa ccagacgccg agcgcgccga gcacaaagaa cgagacggcg ttggtcaccg
     2581 cagccatctg ccagcaactt gcgaagagcg cgaaatgaac gagcgccttc gatgtctgcc
     2641 cgttgtaggc tgcgcccaga ccggggacga ggagcgatag cagcgtcgcc acccacttcg
     2701 aggtgcggcg tcgcaactgc tgcggcgcgt ggagcagcgc cgtctgttgc tgcaagagtt
     2761 cgacgcccgc cacgatgcag tcctggcaat acggaaaccc gcgtatgcgg tgatcgcacg
     2821 cagggcacag aggccgcgcg caccgactgc actgcacgac cgacgcattc atgttgtgat
     2881 aagaacagtt catcaacttc tctgtgttac tcgctgctgc cgtaactaaa cttgcttcca
     2941 cgattcgttc atacttcgag caaacaattt catcaatgcg tctcgaaccc gcgcccggtt
     3001 tggtcctaac gcttttgcgc cccggtttgt tgctccgatg cgtccgagcc gttcgctgcg
     3061 ggctgcgcgt tctgttgctg cccctgctcc gtctccttct cgttctgcgc gggcgagccg
     3121 agcagacttt cgaggctgcc cgccaccttc ttcaaatcgc ccgacattac tgcgcgacgc
     3181 acgggcaaat tcgcgccgcg ctcgtaagtt ctcgccgcga agcgcagact cgcgcggtac
     3241 atgccgccga ttgaaccgtc gtcagagagc gtgctcgttc cgacgagcac tgcgacgagt
     3301 agcattgtgg cgacggtggc gagttgcggc gagacgattg cgtcgagcca gccgcgcacg
     3361 agttcggcga ggcgagccga gagcggcgcg cgaagttgtt ccgcctcggt cgtgccgagc
     3421 gtggcttgca ggatgcgcgc gtgcagcgcg gcgggcacag ggcgttcctc gttgatgtag
     3481 gtgtagcacg cgccgatgga gcgcacgact tcgccgggca ggttcgtgca actgtcgcag
     3541 agcgcggcat gacgctccca gcgatggtag agcgacgcgg gcaaaaatcc gtcgaggtaa
     3601 tcggttaggt aatcttcaaa ttcgtcgcac gtcatcgccg actcgggcgc ggtgtgcaac
     3661 aggatgcgcg cgtcgagact ggtcgaaggc gcgggcacgg tcgccgtacg gcaggcgcgc
     3721 agcgtgtttt tgacttcgct taaaagatcg tgacagacgg gacaccggag ggcgtgcccg
     3781 ttaaacgcga tctgcgtcgt cgtgtcgagc gagccttcga ggtaatccgt cagacgatct
     3841 tcaaattcag cgcacagcat ttttttctcg tttcaccctt ctgattaaat gatgaacgcg
     3901 gaacgatgaa cgatgaatga aagactcaaa gtgataatgg ccgatttaaa cttcatcgtt
     3961 cctccttcac gtcgttccgc gtttaactaa acgtcgctca tggcgacgac gcgcatgcgt
     4021 cttaaaattt ttgccagttc aattcgcccg cgattgatgc gcgacttcac agtgccttcc
     4081 ggtatctgca agagatcgac gatctcctga tagctcaact cttcgatgtc gcgcaggatg
     4141 acgcaggtgc gcaaatcggg agcgagtttg tcgattccgg cctgcacctg ctcgccgagt
     4201 tcgcgccgtt ccatgtcaac gtgcggcgaa gagttggcgg cgcgcaggtg atacgtgtgt
     4261 ccttccaaat cctcgccctg cgaatcctgc ggggcgcgtt gacgcttgcg gtaatcgtca
     4321 atgacgagat tgcgcgcgag gcgcatcaac cagttttgca ggtcgccctg cttcgggtcg
     4381 tactgttcga gcgaacgata gacgcggacg aaaacttcct gcgtcaaatc ttccgccgca
     4441 tccgtgcgcg aggtgaagcg ataggcgaga ttgtagatgc ggcgggaata gagagacacg
     4501 atctcctccc acgccgcgcc atcgcccgcg cgacatctcc tgaccaattc agcgccatca
     4561 ttgaccgtca aagcctttgc agctcccaac actttctgac gccctttccg cctcctgccg
     4621 cgagccggaa attgaaggcg cggcggcgaa gaacttgatt gccgtacccg tttacgacgg
     4681 gcggaaaggg aaagttcccc gtaaatccag cggggaaaag caataagccg gaggcgtccg
     4741 gcggcgaccc ggagcggttc gcgcgagcgt ttttgaagcc tctgaaaatt cgccgcgaca
     4801 gctttttccg cttttttccg cccggcggac ggggtggcat tccactccga caagcggcgt
     4861 acgcctcccg tctgccgggt tgcgtccccg ttctatgctc cggtctgcct tcttgcgact
     4921 tgatcttgaa tactgtagcc ttacaaggcc acccaatata ttgtggtcgg taagttgtga
     4981 gaggtcaaga gacgcgcgac gatagcgcag aagtttcgtc gaaaaattac ggagaggacg
     5041 cccgattttg cgtcgttatc tcaaatttat aattttgact ctcgtggccg ccgccgtcct
     5101 ctggtggttc gggcgcgggc tggactgggc gagcgtgcgc gcggccatcg gcaagtcgga
     5161 ttggcgtctg atcgcgctcg ccgtcctcat cgtctggggc acctacttcg tgcgcgcact
     5221 gcgctggcgc gcgctccttg cgccgctcac cgaagcgagt ctgcgcgaac tctttgccgc
     5281 gacaaccgta ggattcagcg cggtcttttt aatcgggcgc gccggggaag tggtgcgccc
     5341 gacgtttttg cctctgcgcg accggcgcgt gcgtccggcg gcggcttttg tgaccatcgg
     5401 cgtcgagcgg atttacgaca tggtggcggt cgtcgtgctc ttcgcggtca atttgatctg
     5461 gtttcgcgca ccgggcggcg gcgacgcggc ggcttatgcg cgtgtgcgtc aggcgggaat
     5521 gcttttgctc gtcgcggcgg cggtcggcat ctttgcgctc gtcgtcttca agcgatactc
     5581 gcggcaagtg atcgattggt gcgaggcgaa atttgcgcgc gctccgccac tcttgaaacg
     5641 cgcgggcaaa ttgttgacgc acttgctcga acaggtcgcg ggtgccttga gcgtgctcgt
     5701 ctcggcgcgt gaactcgctg taacggtcgg gctgaccgcg ctcctgtggg gcatcatcgc
     5761 agtggcgaac tggctcgtac tgcgcgcgtt cgggttgccg ttcggcgcga cggagactct
     5821 gttcgtgctg ggctgggcgc tcgtcgggtc gctcgtgccg acgccgggcg gcgcagccgg
     5881 agcgttccac gccgccaccg ccgccggtct gatctttctc ggcatcgcgc gcgaagaggc
     5941 ggcggccatc tcaatcgtgc tgcacctcgt cgtcttcgcg cccgcagtgg tcttcggcct
     6001 ctactatttc ctgcgcagcg acgtgagctt tgagcgtctg cgaagtttga cggcaaccga
     6061 accgcacgaa gacgaagagg aagagcgcgg cgacgaacgc gactcgactc aggcgggacg
     6121 ggcggcaaaa gacaaggcgg cactctccga gctttcgcgc gacaggagcg cgggagcgga
     6181 gacttagagc gagcaagggc aaaccggcaa gcgaggtcag gcgcgtcgtt tttgggcgag
     6241 ccttttggcc tcaactt
//
LOCUS       DASWNS010000478         4154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_407209, whole genome shotgun sequence.
ACCESSION   DASWNS010000478 DASWNS010000000
VERSION     DASWNS010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407209"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1119
                     /locus_tag="VGB76_16435"
     CDS             16..1119
                     /locus_tag="VGB76_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY0100535.1"
                     /translation="MSESLKVKSKENCRWDMVSLGEVMLRLDPGDARVHTARSFQVWE
                     GGGEYNVARGLRRCFRLDTAVVTALADNPVGRLVEDLIFQGGVDQSHVLWRKFDGVGR
                     EVRNGLNFTERGYGVRAALGCSDRGHTAVSQLRPGDIDWDAIFGGEGARWFHTGGIFA
                     ALSETTPAVAREAMEAARRHGTIISYDLNYRDSLWKGIGGKARAQEVNRELARFVDVM
                     IGNEEDFTACLGLEVEGLDEHHSKLDVGNFRKMIERAVSDFPNFKAVATTLRNAKTAS
                     INDWGAVCFYEGELYQSQTRENLEIYDRVGGGDSFASGLIYGFLAGKGAQWAVECGAA
                     HGALAMTTPGDTTMATLSEVERVMKGGGARVAR"
     gene            1177..1836
                     /locus_tag="VGB76_16440"
     CDS             1177..1836
                     /locus_tag="VGB76_16440"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005119.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEY0100536.1"
                     /translation="MNKNEVLRRIEEVGVVPVVRASSAEEAMQVIEAIRAGGVSVLEI
                     TMTVPRAVRVIEQVADRYGAEVVVGAGTVLDAETARACILAGATFVVSPSLNTGTIEL
                     CRRYSVAVMPGALTPTEVVTAWQAGADVVKVFPCGALGGAKYLKSLKAPLPQIEMVPT
                     GGVSLQTAAEFIEAGALALGVGADLVDTKAIRAGQPEKVTDAARAYVAAVRAARNQTA
                     T"
     gene            complement(1960..2775)
                     /locus_tag="VGB76_16445"
     CDS             complement(1960..2775)
                     /locus_tag="VGB76_16445"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY0100537.1"
                     /translation="MTDLNNDIFKTIDAERRGTTSEEVVSQLREMIHRGELRPGDRLP
                     PERDLAKMLGVSRPTLRAGIRSLAAVGVLQSRQGAGTFVVKSDGPPSLDSSPLRLMAS
                     LHGFTSAEMFEARQSLEMAVAGLAAERATDDQMATMAEETTGMFASLDDPAQFLVHDM
                     HFHQTVAAASGNRILTSLMNMVATILFDVRSKTVKRARDLKESAEMHRHIYRAIRERN
                     PEAARKAMREHLMLAQKAQEQEAADDAAGRNGANGANGANSTNGANGANGDVH"
     gene            3128..>4154
                     /locus_tag="VGB76_16450"
     CDS             3128..>4154
                     /locus_tag="VGB76_16450"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEY0100538.1"
                     /translation="MKIILRVILYAILSSVLAASVLGQTPRLSERMAATAMNSLYRDA
                     GKKETGKPSKWTYDLGVVLKGIEGVWYATGDGRYFKHIQENLDHWVQPDGTIRTYKVE
                     EYNIDHILLGRNLLTLYKVTGQEKYRKAAGLLREQLRTHPRTSEGGFWHKKIYPHQMW
                     LDGLYMGEPFYAEYAATFNEPEAFEDIAKQFVLMERHSRDAKTGLLYHGWDESRQQRW
                     ADKTTGRSPNFWGRAMGWYAMALVDTLEHFPAEHPRRAELIAILNRLAAAVTKYQEPK
                     SGLWYQVVDKGGHKGNYLEASASAMFVYSLAKGVRQGYLPASYLKVAEKAYRGMVKEF
                     VKPVSEAQ"
BASE COUNT          777 a         1252 c         1362 g          763 t
ORIGIN      
        1 agataggtgg agactatgag cgagtcactt aaggtgaaat cgaaagagaa ttgccgttgg
       61 gacatggtga gcctcggcga agtgatgctg aggctcgacc ccggcgacgc gcgcgtccac
      121 accgcacgct cctttcaagt ctgggagggc gggggcgaat acaacgtggc gcgcggcctg
      181 cgccgctgct tccggctcga caccgccgtc gtcaccgcgc tcgcagacaa cccggtcgga
      241 cgcctcgttg aagacctcat ctttcagggc ggcgtagatc agtcgcacgt cctgtggcgc
      301 aagttcgacg gcgtggggcg cgaggtgcgc aacggcctca acttcacgga gcgcggctac
      361 ggcgtgcgcg ccgcgctcgg ctgctccgac cgaggccaca ccgccgtctc ccaactacgc
      421 cccggagaca tcgactggga cgccatcttc ggcggcgagg gcgcgcgctg gtttcacacc
      481 ggcggcatct tcgccgcgct ctcggagacg acgccagccg tcgcacgcga agcgatggaa
      541 gccgcacgcc gccacggcac gatcatctcc tacgatctga actaccgcga ttcgctctgg
      601 aagggcatcg gcggcaaggc gcgcgcgcag gaggtcaacc gcgaactcgc gcgctttgtt
      661 gatgtgatga tcggcaacga ggaagacttc acggcctgcc tcggcctcga agtcgaaggt
      721 ctggacgagc accattcgaa actcgacgtg ggcaatttcc gcaagatgat cgagcgcgcc
      781 gtcagcgact tcccgaactt caaggcggtc gccaccacgc tgcgcaacgc gaagacggcc
      841 agcatcaacg actggggcgc ggtctgtttt tacgagggcg aactttatca atcgcagacg
      901 cgcgagaacc tcgaaatcta cgaccgcgtc ggtggcggcg actctttcgc ctccggcctc
      961 atctacggct ttctggcggg caaaggcgcg caatgggctg tcgagtgcgg cgcggcgcac
     1021 ggcgcgctgg cgatgaccac gccgggcgac acgacgatgg cgacgctctc ggaagtcgag
     1081 cgcgtcatga agggcggcgg cgcgcgcgtg gcgcgttgat ctcaggctcg cagtcatgtg
     1141 cggtaatttg cgcgcgatga ttcaagtggg gaagcgatga ataagaatga agtcttgcgg
     1201 cggatcgaag aggtgggggt cgtgcccgtc gtgcgtgcgt cttctgcgga agaggcgatg
     1261 caggtcatcg aggcgatcag ggcgggcggc gtcagcgtac tcgaaattac gatgaccgtg
     1321 ccgcgcgccg tgcgcgtcat cgaacaggtg gccgaccgtt acggtgcgga ggtcgtcgtc
     1381 ggcgcgggca ctgtgctcga tgcggagacg gcgcgcgcct gcatcctcgc gggagccacg
     1441 tttgtcgtca gtccttcgct caacaccgga accattgaat tgtgccgccg ctactccgtc
     1501 gccgtcatgc ccggcgcgtt gacgccgacc gaggtcgtga cggcgtggca ggcgggcgcg
     1561 gacgtggtca aggtctttcc ctgcggtgcg ctcggcggcg cgaagtatct gaagtctttg
     1621 aaagcccctc tgccgcagat cgagatggtc ccgaccggtg gcgtctcgct ccagaccgcg
     1681 gcggagttca tagaggcggg cgcgctcgcg ctcggcgtcg gcgcagacct cgtggacacg
     1741 aaggccatcc gcgccggaca gccggagaag gtgaccgatg cggcacgcgc ctacgtcgcc
     1801 gccgtgcgcg ccgcacgcaa ccagaccgcg acttagatgt gagttgaatt cagagcggga
     1861 agatgtttga caacttgcag ccgggataga gcgttagtaa ggagcgcgcc tccgactcgt
     1921 catttcggaa accggaacgc gaaacttaat gcgcgtcact taatgcacgt cgccgttcgc
     1981 tccgttcgct ccgttcgtgc tattcgctcc gttcgccccg tttgcgccat tacgtccggc
     2041 ggcatcgtca gccgcctcct gctcctgcgc cttctgcgcg agcatgaggt gttcgcgcat
     2101 ggccttgcgc gcggcctccg ggttgcgctc gcggatcgcg cgatagatgt gtcggtgcat
     2161 ctctgccgac tctttcaggt cgcgcgcgcg cttgacggtc ttgctgcgca cgtcgaagag
     2221 gatggtcgcc accatgttca tcagcgacgt gaggatgcgg ttgccggagg ctgccgccac
     2281 cgtctggtgg aagtgcatgt cgtgtacgag aaactgcgcc gggtcgtcga gcgatgcgaa
     2341 catccccgtc gtctcttcgg ccatcgtcgc catctggtcg tcggtggcgc gctcggcggc
     2401 aagccccgcg acggccatct caagcgactg ccgcgcctcg aacatctccg ccgaggtgaa
     2461 gccgtgcaga gaggccatca gtcgtagagg gctgctgtcg agcgacggcg gcccgtcgga
     2521 tttgacgacg aaagtgcccg cgccctgccg cgattggagc acgccgacgg cggcgagcga
     2581 acgaatcccc gcgcgcagcg tgggacggct gacgccgagc atcttggcga ggtcgcgctc
     2641 gggcgggagc cggtcgccgg ggcgtagttc gccgcgatgg atcatttcac ggagttgcga
     2701 gacgacctcc tccgaagtcg ttccgcgtcg ctcggcgtct atagttttga agatgtcatt
     2761 gttgagatcg gtcataccat tagctctgta gagatatttt tcagaccaca tgcggcagga
     2821 tgattgcacc ggcctccgag gcgaccaatg agcgtttcat gagcggacgg attttaactc
     2881 ttccgctgcg agttgtcgaa caatttatca aacgcattca tcttaaaggt ttgaccattt
     2941 cggaaatttc aaaaacgact ctgaaattgt tcatcgtccc ctctcggaaa ttggtcatcc
     3001 gacccggcga cggagcccgc ttccagtgcg taaatttcgt gctcgcaagc gggcgctgtg
     3061 aacttcgata aagggacttt cgaacaaggg catagtctgt gtgagcgaac tgccggaggg
     3121 aatatttatg aagattattt tgagagtcat cctctacgcc attctgtcga gcgtgttggc
     3181 cgcgtcggtc ttgggacaga ccccgagact ctccgagcgg atggcggcca ccgccatgaa
     3241 cagcctctat cgcgacgcgg gaaaaaagga gacgggcaag ccttcgaagt ggacttacga
     3301 tctcggcgtc gtgctcaagg gcatcgaggg cgtctggtac gcgacgggcg acggacgcta
     3361 cttcaaacac atacaggaga acctcgatca ctgggtgcag cccgacggga cgatccgcac
     3421 ctacaaggtg gaggagtaca acattgatca catactgctc gggcggaacc ttttgacgct
     3481 ctacaaggtg acggggcagg agaagtatcg taaggccgcc gggctgctgc gggagcaact
     3541 gaggacgcac ccgcgcacct ccgagggcgg cttctggcac aagaaaattt acccgcacca
     3601 gatgtggctc gacggcctct acatgggcga gcctttctac gccgagtacg ccgccacctt
     3661 caacgagccg gaggcgttcg aagacatcgc caaacagttc gtcctcatgg agcggcattc
     3721 gcgcgacgcg aagaccggcc tgctctatca cggctgggac gaatcgcgcc agcagcgttg
     3781 ggctgacaag acgacgggac gctcgcccaa cttctgggga cgcgcgatgg gctggtacgc
     3841 gatggcactc gtggacacgc tcgaacattt cccggcagag cacccgcgtc gtgccgagtt
     3901 gatcgcgatc ctgaaccgtc tggcggcggc agttacgaag tatcaggagc cgaagtccgg
     3961 cctctggtat caggtggttg ataagggcgg ccacaagggc aactatctcg aagcgtcggc
     4021 ctccgccatg ttcgtctact cgctggcgaa aggcgtgcgc caaggctatc tcccggcctc
     4081 atacctgaag gtcgcggaga aggcttacag agggatggtc aaagagttcg tcaagccggt
     4141 ttcggaggcg cagg
//
LOCUS       DASWNS010000479        19304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_328279, whole genome shotgun sequence.
ACCESSION   DASWNS010000479 DASWNS010000000
VERSION     DASWNS010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 19304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19304
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328279"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VGB76_16455"
     CDS             complement(<1..692)
                     /locus_tag="VGB76_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100539.1"
                     /translation="MKKSSGSLFCRGLIVVLLAGLVCVVNCGGVAGDPVANQTAGDGS
                     ARTSANVSPSASDARPSPNPERVEPAQGAERGSGVLIDYRKGQPDHRTPPLDAQLRRR
                     IVQSAYGAKAAPDDYSINSNSSGSFTAQGARETVYLVQRGGAVAADPNGAQDLALVVF
                     DASEQLVAKFKTSDFNFIAATSDTDGDGVNELLLEGSFYNMGTLGSSARLVELKSKQL
                     RLIETFEGIYENS"
     gene            complement(737..1267)
                     /locus_tag="VGB76_16460"
     CDS             complement(737..1267)
                     /locus_tag="VGB76_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100540.1"
                     /translation="MNWLQLTTRALCLFMLLGVVFTLRAATTPHAPPRASASHNARAP
                     QDQLRCTPRRLRRGDTLTLTMPARHGGYLAIVNPRGKYFFLTSDDSGARTFEKNAGVN
                     PSLSATEFGGMQQLQLSTTDTKAIDYEGSQEKRRAETVFRQTGWYKVLISDTSLERDE
                     PSPQGQCRVYYVNKSR"
     gene            complement(1382..3286)
                     /locus_tag="VGB76_16465"
     CDS             complement(1382..3286)
                     /locus_tag="VGB76_16465"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY0100541.1"
                     /translation="MSRIEFLRYEFFESLFSPQADDATETSSDVAAPTLAVGTADGLE
                     TFLAQTGLQSGSRKMRLQTFGANNEVAPAQTPASDFDLDTIQGVAGNRNITPEFRREV
                     AAMARRLGTRPEYIMAVMSFETGGTFSPAKENRSTHATGLIQFMPATAQGMLAEEGIN
                     ITRSRAREIFAAMTPVEQLRYVERYFAQSKFNNRLGTLEGTYTAVLSGRARANGDDVV
                     FGPGDAYNANAPLDWNRDGQITASEATSPVAFRLFGGVTKVQERLRDLGLYDRTPDGS
                     YGARTRAALEQFQREHQLAPTGLMNEATGRALGLAGEETNPTERTPGDLARGATGAEV
                     ERLQDQLVAFGHLTAADKATGPGIFGGRTENALEAFQRANRLTDSGVYDEATRNAVAR
                     LNDGVRRGSDQRNIVSGLQDRLVALGYLTSAEVATGPGIFGQKTENALAAFQRRNGLD
                     PSGVLDAATYRRLHSSNAVRNERQAAEGGHFTVDNGVRLSDAARERVGRIADEYFRRT
                     GKNLRVTDGTRTAHDQARQIYNKIQLNDTGIYRNQTALAEIRRAYDAGVRAGKSRAEI
                     INDMSAVIQGQVDRGVYISRHLRAGAVDISVRGMTAADEREFRASVAASSAGTSVLRE
                     TRPPHFHLQF"
     gene            complement(3430..4341)
                     /locus_tag="VGB76_16470"
     CDS             complement(3430..4341)
                     /locus_tag="VGB76_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100542.1"
                     /translation="MTFDGQSAARSSDYDLRSLQHERRMLAEYRRGAAHDGALLQPLA
                     NACWRAAAFEWQLYANAETVRQLWGESARALAQGFTRRRPGFDPSPDQLILAFHFSIA
                     AREREAFTALALANPDTRGRALREARAFRGSRAHFHLAEGYAQVARSLVERQAAPARA
                     AAGSLEAALAESDHGWWHRQFPTALDAAWRISEHEAFCVLLGAIARRLISSFAGETEE
                     SPGQTDEDAAAEFARVVDETLLRLEQFIEYDPNHHPKLYLWLPGLALCALAASAGLPV
                     AWLHERHEENAGGYTRLPLALLRNPTA"
     gene            complement(4483..6138)
                     /locus_tag="VGB76_16475"
     CDS             complement(4483..6138)
                     /locus_tag="VGB76_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100543.1"
                     /translation="MGGVNFKPGVHGTGQHPAGGAQGDGDGVHGNSGKTNPHDEHGRE
                     SQGQSRRNGRVAARVGELAAQTQHVADKIKRGNGHQPKSDSTTHPDSNSRSNRSHGNH
                     RNGDQHGRVERGNQTHAHDSHGRDVHGRDVHGRGHDSSLTPAAVKYLRGESGNGALPV
                     NVRGALDTLKTALGQKPLEELLSERGHKVEKLIDHFLRQSERASERVGEHGRRTVHQA
                     ADELFSAVQLEKHFARLERTGGDAARRAEEAVFRLLFNAHGELHNDHQRAQQRDRQVT
                     ELWRDLRSGAFLPAQDSYSQFQLTGRARVVNEMIELLHTLDALERGASQQFPGGAKGA
                     GQQGALAETLAGRLANLTEAELAELLNLLPTLPGRAGRNEMVRLLAALSGPLMDADGK
                     MLLAKDGTPLKPDQLLWLSTVGGLPGSGFKGELYGTHLSPLIVFGFDALYSLIGFDGR
                     TLVPPHFAAVQAQINNSELEWVCGQPPLTEGWMHALIERLKDSAEADQNLLGEMLEEA
                     LVDGRFHAVLVQGSVAEGEAVSGSFSVTRLLPDPSAAQSFATS"
     gene            complement(6268..7464)
                     /locus_tag="VGB76_16480"
     CDS             complement(6268..7464)
                     /locus_tag="VGB76_16480"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0100544.1"
                     /translation="MAGIDGINRSGRPASDDGGDKLVRRETPFVRNRRTNFEQGFERG
                     LAADAQAVTPKHNHAAGERDGGNGASSYLSDEAWTRQRTLEELKTLVQAIQSGVTDPW
                     QLTDLIFYARHPDMIGQPLTKDQQPLLDEWNAISALLVHPAINEVGDFLGANVIGGDV
                     RGARGMETAFERATHSPINEGRLANGSTRAYDDVIARAVEWCPGLSPAILKGLLAQES
                     NFNPTVINQYGYAGIAQFGREAAREVGLRVGIAGSAADERLNPAKAIPGAARLLNIKA
                     QRLGQIAFARYGQPNGVEFWKFVLAAYNGGEGTVALAMGHAYRAGLASARTRGLVGAE
                     AVSFARSYASKWENLKAGGADSPLAQASARYFPTLAAAKFKEIGNYPTAIVARALGSH
                     LSGQKA"
     gene            complement(7514..8062)
                     /locus_tag="VGB76_16485"
     CDS             complement(7514..8062)
                     /locus_tag="VGB76_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100545.1"
                     /translation="MSDPVFGIMSQAISQQVGGAAQQPAQQKEGARSFESVLQEGGGQ
                     GGDLHNPAGQPTQVSGADLERMRVDLQQRIERLPADTSNVKALLPEFIDSRTRLSLLR
                     EAIGSAGNTPKGTDLRGQFAKVEGEWHQIESIMKSDKELSQGELLGLQARLYQVSQHV
                     EVMSKVVDQVTGGVKTILNTNV"
     gene            complement(8222..8695)
                     /locus_tag="VGB76_16490"
     CDS             complement(8222..8695)
                     /locus_tag="VGB76_16490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100546.1"
                     /translation="MSERLLNDWLQGRASLAAASEWTTEEMRLVADLGYALAEQGREQ
                     EALTIFEGLSALAPATAYFQAALGALRLRLGEPQLAIGHLDAALASDPQNISALVNRG
                     EACLQLGEYAAATRDLEAALRLGETFPSEDETLRLARARALLFRLRTLAGGTAGA"
     gene            8910..9350
                     /locus_tag="VGB76_16495"
     CDS             8910..9350
                     /locus_tag="VGB76_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100547.1"
                     /translation="MFGLGKLVGGALNAIGLGKIAPFISMGISFFTGDFASLATDVMG
                     LMSNIKGLDFLNKVAQFAPLGGFDQMAGGCFGDLGLKQLLKADGLRDIADRFFKIADK
                     LEDFEASSQKVNDLFDLLLDTAKNRESLENVRSSVTYSSYISAV"
     gene            9388..9864
                     /locus_tag="VGB76_16500"
     CDS             9388..9864
                     /locus_tag="VGB76_16500"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100548.1"
                     /translation="MTRQQSNGKQTPGGDAALPTLRERLGISEEQFREMGQVGAMYYK
                     QGNLEKARTIFEGLVEVDPQSVDAHSALGALYTRTQRDAEALHHLNRALELDDRQIAA
                     HVNRAEIYLRRQQVEEAVADLKKAIELDPHEQDAGANRARAMVLGIHEALEAKGVM"
     gene            9912..10136
                     /locus_tag="VGB76_16505"
     CDS             9912..10136
                     /locus_tag="VGB76_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100549.1"
                     /translation="MSNSIQGFNIPAKPNPQDFDFTDPQQAAAYQEKSQNYHFAVDMM
                     MNAIKQEGEAKSNLLKTINDGLDAHIRNLG"
     gene            10382..10630
                     /locus_tag="VGB76_16510"
     CDS             10382..10630
                     /locus_tag="VGB76_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100550.1"
                     /translation="MTKLMRAITISCALVVLLAGSALAQYGGRYRGGGGTTYRRPSTA
                     TSSLILRRSIQRAQVRRQKARALKRRQANAANRRHTQH"
     gene            10657..11481
                     /locus_tag="VGB76_16515"
     CDS             10657..11481
                     /locus_tag="VGB76_16515"
                     /inference="COORDINATES: protein motif:HMM:NF042732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6683 family protein"
                     /protein_id="HEY0100551.1"
                     /translation="MRFYEQRLARRALLPFLLLCLAASVASAQTYSRGTYSGANSTRW
                     NNPMSASLNRMLNRNILNQSIRRRHSMGSARATSGNNAPRTTNTRTTPNGTVKPVNST
                     PANNSATAFRPVASSLYPQQLAREAATDEAERKEMEAVFAMCLRNYEANMRRQGQPLK
                     DVARAVSYFIGVNYNVLHGGGTVTPAQGVALRAGIQAALGEDESFRRLSDREKQQIYE
                     TMAVLAEFVALNISVADERGNKQLAEATRSMAADNLEKLLGASAASIRITDRGIEF"
     gene            complement(11603..12454)
                     /gene="fliR"
                     /locus_tag="VGB76_16520"
     CDS             complement(11603..12454)
                     /gene="fliR"
                     /locus_tag="VGB76_16520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEY0100552.1"
                     /translation="MDEFLKLLETILRTFEVGQSPQSFLVLIGLAFARMLSFLTVVPF
                     FGGAAVPARVKVATATAFVIILYPSLEAGLPKDGAPLGFGPVGFIGLLVKEAFVGFTL
                     GFVASLVFEAIQVAGRVIDFQRGSTLAEMFAPQLQTRVSELGQFKLQLAIVLFFMIGA
                     HHAFITSLLRSFEIIPAMSFPHLQAGWSPAAAFITKLTGGVLAVGIQLAAPAMIALLL
                     TDLFFGLVNRVAPQINVFFLSLPVKMAVGLVVVLAALPLYRERYIYYFKEAYRAFEAV
                     LKTIGGG"
     gene            complement(12599..12868)
                     /gene="fliQ"
                     /locus_tag="VGB76_16525"
     CDS             complement(12599..12868)
                     /gene="fliQ"
                     /locus_tag="VGB76_16525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEY0100553.1"
                     /translation="MGTYVSLAAEALWLILLLSLPPLIASLVVGLVISLLQALTQVQE
                     QTLTFVPKIIATLLVLMISANWMLDRLLIFGHKVFAMMPYVGKGM"
     gene            complement(12896..13693)
                     /gene="sctR"
                     /locus_tag="VGB76_16530"
     CDS             complement(12896..13693)
                     /gene="sctR"
                     /locus_tag="VGB76_16530"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HEY0100554.1"
                     /translation="MNTKPAVWKKLAGITLIGFVAMCALAAFTTVDAHAQAQVPARDG
                     GTANPVVLVIVLGALALAPFALIMLTSFVKISVVLSILRNALGTQQVPPNQVITGISL
                     ILTIFIMSPVVEKMYAAAGNIGDSQAIFSEVSVRTLFEAAQRGREPLRAFLERHAHER
                     DRKMFFDLARRMAVKNGNDPNEINVSDFRTVIPAFVTSQLSEAFQIGFLLFIPFLIID
                     MVVANILQAMGMFMLSPVMISLPFKLLLFVLIDGWVLLIQNLVLSYT"
     gene            complement(13697..14170)
                     /locus_tag="VGB76_16535"
     CDS             complement(13697..14170)
                     /locus_tag="VGB76_16535"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY0100555.1"
                     /translation="MDAPGLLHGVCASVLAALQAPTPFDPAQYNDSGASFFAMLAQTI
                     VALALVCGLAYVLFRWVLPRLQQVGGASGSMVRIVDRTGLDTRKSLYVIEVAGRWLLI
                     ASSEAGVQLISELDATSAEEAAHEVERLRPNFKQSTATARDAVAERFSRLLGKRK"
     gene            complement(14170..15600)
                     /gene="sctQ"
                     /locus_tag="VGB76_16540"
     CDS             complement(14170..15600)
                     /gene="sctQ"
                     /locus_tag="VGB76_16540"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system cytoplasmic ring
                     protein SctQ"
                     /protein_id="HEY0100556.1"
                     /translation="MKADATDKAGTEAPTVREGTEVGDPFAVDLFARGRHSAGAPAHV
                     AAPDAMADLFADTDARAAWSIRLPRVSRAELRLSSALGASLPTLSAGVRATLVRVLSQ
                     YLREPEDEIVVSQADVREREFSSNSTSTGGTVDAWTTLALAPANASLFASLQTDFASA
                     LVDRMLGGEGHAPETLRLLSRTELAAFEFLWLCLIRELHRQVDAPLWRLEGVSTEPPA
                     SLKGGPGVQSNTRAPGNATKEQTGARAIVATFALRVGALSGLVSFYFTRDALAALEGL
                     RHSPLFAGRERDARRELFKRVAPDVRLRPVLGETDVRAIDLADLESGDVVVVARPLGR
                     FEAGRLSGRLEMCLGDGAGAILTGTLTMPDDDAAQLLIEQIKRGGELHGTERWDMEQE
                     QTSDEDSNESAIALEEILLTVRVELAERRLSLDELARLRVGQLLPLGCQATDPVDLVA
                     DGRRVARGELVEIEGRLGVRVTQVLR"
     gene            complement(15680..16483)
                     /locus_tag="VGB76_16545"
     CDS             complement(15680..16483)
                     /locus_tag="VGB76_16545"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY0100557.1"
                     /translation="MKVTPPKNTPEPPPTTNNITSRRVSSQTNTVPHEDDGALASVHT
                     TRDFASVLEDVTRTAERDDNESSEGERQETKSSAHAERERESKRREDRHDGGTGGGGF
                     EQRGQVRDAGHISEAAPARAILHIADLERIVAAVRTQLISGGRQEVTLELHRSVLEGL
                     RVRLTTDGQGRVNAEFITASEKLRAQLDARAGDLSDLLRGRGVELASLRTTVGANGDQ
                     TTSGDGGRHAPDNPPRVLSGRDGAHASAAAEDASQDERAADESTSSYRA"
     gene            complement(16540..17883)
                     /locus_tag="VGB76_16550"
     CDS             complement(16540..17883)
                     /locus_tag="VGB76_16550"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100558.1"
                     /translation="MRAAICAGLLLVLLGSQHVAVSQKKPSIADDAASTEKKNGGASK
                     ENANKQTDATRKGNRRRQRPAPAAALLEVVFTTDMPDSDIFLNSPGGTPQKLGKTDAD
                     GKLSYKLPRGSHSVTVSHIGQRTERQQIDVRPGNTNFSFNVALPTPKASPEEAKTDAT
                     PAPADDTAAKEAESAANAVEEIIKRHLDAQQTANVSASDWQIVETYNTSASEKDPANK
                     QLRARALFAQGQLAYLRGDYPGALVAFNKAVLAEPELVAAHYSLGNAYLATNQPAQAV
                     RAYQRAAELNQELALVYKGLGDAFTKQGKNKEANSYYARAKRLGPMPTSSSLDSTRLL
                     MKRKQWSAALRELLEISKTQPSADIYIYIGDCYDGMKQPLSASQAYQKATELDPKSAL
                     AAYRYGEKMFELREFAATMEALERALALDQTGVTINRKRAREMANQAAEQLRKMK"
     gene            complement(17993..>19304)
                     /locus_tag="VGB76_16555"
     CDS             complement(17993..>19304)
                     /locus_tag="VGB76_16555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100559.1"
                     /translation="GVQQFKILVSALRVAWCAALLTSLLCLTNALAAPQDRPSIAGDV
                     KNSGKKPKADATNGGKRAASTRPTPRRNPSPAVPRPSALLDVTFWTGVAESDIFVHAP
                     GGKLQSLGKTGADGKLRTRMPRGVYNVTASRAGYNAQRQQISVQPGSANFILNLSGQP
                     LPGTLPTAAEVLKRFSDPKQTDRVTVAEWEAAQQQTALAFAANPGDAQVRAQSLFAQG
                     QLAYLRRDYAGALVAFNNSALALPTSALAYYGLGNAYFATNQLPEALRAYQRAIELDG
                     QLAMAHRGVGDVLTRQGKSKDALTYYERAKTLGYFSASASLSTGRNLMRRGRWAEAIR
                     ELSDLSKVQPSAEVFIALGDCYMQLKQPLSAAPAYRRAIELDSGSAQAHARYGEMMYG
                     SREYAGAAEALERALALDQAGTTINRARTRKMADDAASKARRVN"
BASE COUNT         3452 a         6058 c         6158 g         3636 t
ORIGIN      
        1 gaattttcgt agatgccctc gaaagtctcg atcaagcgca actgtttgga cttcaactcg
       61 accagccgcg ccgaactgcc gagcgtgccc atgttgtaga agctgccttc gagcaacagt
      121 tcgttcacgc cgtcgccgtc cgtgtcgctc gtcgcggcga tgaaattgaa atcactggtc
      181 ttgaatttgg cgacgagttg ctcggacgcg tcgaagacga cgagcgcgag gtcttgcgcg
      241 ccgttggggt cagcggcgac agccccgccg cgctgcacca gatagactgt ttcgcgcgcg
      301 ccctgcgccg taaaagagcc gcttgagttg ctgttgatgc tgtaatcgtc gggcgcggcc
      361 ttcgcgccgt aagcggactg gacgatgcgg cgtcggagtt gtgcatcgag tggaggcgtt
      421 cgatggtctg gctgtccttt gcggtaatcg atcaacacac cgctcccacg ctccgcgccc
      481 tgcgcaggtt ccacgcgctc cgggttcggc gacggacgcg cgtcgctggc ggacggcgag
      541 acgttcgcgg aggttcgggc gctgccgtcg cccgccgtct gatttgctac cgggtcacct
      601 gcgacgccgc cgcagttaac gacgcagacc aaccctgcca agagcacaac tatcaggccg
      661 cgacaaaaga gcgagccgga agatttcttc atcaatcgca tagttgttcg ccgggaagtg
      721 tctctgctct cgtctgtcaa cgactcttgt tgacgtaata aactctgcac tggccttgcg
      781 gggaaggttc atcgcgctca aggctcgtgt cgctgatgag cactttgtac cagccggtct
      841 gccggaagac cgtttcggcg cgtcgcttct cctgcgagcc ttcatagtca atcgccttcg
      901 tgtctgtcgt ggagagttgc aactgctgca tcccgccgaa ctctgttgcg ctgagtgaag
      961 gattgacgcc cgcgttcttt tcaaatgtgc gcgcgcctga atcgtcggac gtgagaaaga
     1021 aatatttgcc gcgcgggttg acgattgcca gatagccgcc gtgacgcgcg ggcatcgtga
     1081 gcgtcagggt gtcgccgcga cgaagcctgc gcggcgtgca acggagctgg tcttgcggcg
     1141 cgcgtgcatt gtgcgacgcg gacgcacgcg gaggcgcgtg tggtgtggtc gcagcgcgca
     1201 gcgtgaacac gacgccgagc agcatgaaca gacaaagggc gcgcgtcgtt agttgaagcc
     1261 aattcatctt cgttcccttc cgtctggcgg tgaatgacga cgcagaaaag tttggggcat
     1321 gaatccttga attgcaagag ttcatgcccc gtccggtgcg ctcccggcgg cgcctgcgcg
     1381 tttagaattg caggtggaaa tggggcggcc tcgtctcgcg cagcacgctt gtaccggcgg
     1441 aggaggcggc gactgacgcg cgaaactcgc gctcgtccgc agccgtcatg ccgcgcacgc
     1501 tgatgtcaac cgcacccgcg cgcaggtggc gtgagatata gacgccgcga tcaacctgtc
     1561 cctgaatcac tgcggacatg tcgttgatga tctctgcgcg cgacttgccc gcgcgcacgc
     1621 ccgcatcgta agcgcgcctg atctctgcca gcgcggtctg gttgcggtag atgcccgtgt
     1681 cgttcaactg tatcttgttg taaatctggc gcgcctgatc gtgcgcggtg cgtgtgccgt
     1741 ccgtgacgcg caaattcttt ccggtgcggc ggaaatactc gtcggcaatg cgccccacgc
     1801 gctcgcgcgc agcgtcggac agcctgacgc cgttatccac cgtgaagtgg ccgccctcgg
     1861 cagcctgccg ctcgttgcgc accgcgttcg atgaatggag tctgcgataa gtcgcggcgt
     1921 cgagcacgcc ggacgggtcg aggccgttgc gacgctggaa ggctgctaaa gcgttctcgg
     1981 tcttctggcc gaagatgccg ggtccagtgg cgacctccgc gctcgtcaag tagccgagcg
     2041 cgacgagacg atcttgcagg ccgctgacga tgttgcgctg gtcgctcccg cgccgcacgc
     2101 cgtcgttcag acgcgcgacg gcgttccgcg tggcttcgtc atagacgccg ctgtccgtca
     2161 gacgattcgc gcgctggaat gcttcgagcg cgttctcggt gcgcccgccg aagattccgg
     2221 gtccggtggc cttgtctgcc gccgtgagat gaccgaacgc cacgagttga tcctgcaaac
     2281 gttcaacttc cgcacccgtc gcgccgcgcg ccagatcgcc cggcgtacgt tcggtcggat
     2341 tcgtctcttc accggcgagg ccgagcgcgc gtccggtcgc ttcgttcatc aagccggtcg
     2401 gggcgagttg atgttcgcgt tgaaactgtt cgagcgcggc gcgcgtgcgc gcaccataac
     2461 tgccgtcggg cgtccggtcg taaaggccga ggtcgcgcag acgctcctgc acttttgtga
     2521 cgccgccgaa gaggcgaaag gcgacgggac tggtggcctc cgaggccgtg atctgtccgt
     2581 ctctgttcca gtcgagcggc gcgttggcgt tgtaagcgtc gccgggtcca aacactacgt
     2641 cgtcgccgtt tgcgcgtgcg cgaccggaga ggacggcggt gtaagtgcct tcgagcgtgc
     2701 cgagacggtt gttgaattta ctctgcgcaa aatatctttc gacgtacctg agttgctcaa
     2761 ccggagtcat cgcggcgaag atttcgcgcg cccgcgagcg tgtgatgttg atgccttctt
     2821 cggcgagcat tccctgcgcg gtggcgggca taaactggat cagtccggtg gcgtgcgtcg
     2881 agcggttctc tttggcggga ctgaacgtgc cgcccgtctc gaaactcata acggccatga
     2941 tgtattcggg gcgcgtgccg agacgccgcg ccatcgccgc aacttcacgt ctgaactccg
     3001 gcgtgatgtt gcgattacct gcgacaccct gaatcgtgtc gaggtcaaag tcgctggcgg
     3061 gagtttgcgc cggtgcgact tcgttgtttg cgccgaatgt ttgcaggcgc attttccggc
     3121 tcccgctctg caacccggtc tgtgcgagaa acgtttccag cccgtcggct gtgcccacag
     3181 cgagcgtcgg ggcggcaacg tcggatgaag tctcagtcgc gtcgtccgcc tgcggggaga
     3241 acagcgactc gaaaaactcg tatctcaaaa actcgatcct gctcatcgtt ggtcctctca
     3301 atgcttttcg ctcacgagga acttgccgca cgtttcgctc tacattggag ttatcggcag
     3361 gcgcgggcaa aagttacgtc cggcgtcgaa attatctcgc gcgtgtgtgc gtggaaagtc
     3421 gggcgcgaat caggcagtgg ggttgcgcag gagtgcgagg gggagacgcg tgtagccgcc
     3481 cgcgttctct tcgtgtcgtt cgtggagcca ggccacgggc agacccgcac tcgcggcgag
     3541 cgcgcacagg gctaagcccg gcagccagag gtaaagtttc gggtggtggt tcgggtcgta
     3601 ctcgatgaac tgttcgaggc gcagcagcgt ctcgtccacc acgcgcgcga actccgccgc
     3661 cgcgtcttcg tccgtttgac ccggcgactc ttccgtctct cctgcaaacg aactgatgag
     3721 gcgacgcgcg attgcgccga ggagcacaca gaacgcctcg tgctcgctga tgcgccaggc
     3781 ggcgtcgagc gcggtcggaa actgcctgtg ccaccagccg tgatcgctct cggccagagc
     3841 cgcttcaagc gacccggcgg cggcgcgcgc gggcgcagcc tgacgttcga cgagcgagcg
     3901 cgcgacctgc gcgtagcctt ccgcgagatg aaagtgggcg cgcgagccgc gaaaggcgcg
     3961 cgcctcacgc aaggctctgc cgcgtgtgtc agggttggcg agcgcgagcg cagtgaacgc
     4021 ctcgcgttcg cgcgcggcaa tcgagaaatg gaaggcgagg atcaactggt cgggactggg
     4081 atcgaagccc ggacggcgac gcgtaaagcc ttgcgccaaa gcgcgcgcgc tctcgcccca
     4141 aagttggcgg acggtttcgg cgtttgcgta gagttgccac tcgaaggcgg cggcgcgcca
     4201 gcaggcgttg gcgagaggtt ggagcaacgc gccgtcgtgc gccgcgccgc gccggtactc
     4261 ggcgagcatg cgtcgctcgt gctgcaacga acgcaggtca tagtcgctgc tccgcgccgc
     4321 gctctgtccg tcaaacgtca taggaggcga gccacccgca catcttatct caccgcgcgc
     4381 gaggcacgag gcgcgtctca agtttatccc acgcctgaac gcgctgccag tggctttcgt
     4441 cgaaggaatc gcaagatcgc agcagagatt cccggagagg ctttaggacg tagcgaaact
     4501 ctgcgcggcg gaaggatcgg gcagcaggcg tgtgaccgag aaggagccgc tcacggcttc
     4561 gccctctgcg accgagcctt gcaccagcac ggcgtgaaaa cgcccatcca cgagcgcctc
     4621 ttcgagcatc tcgccgagca ggttctggtc tgcctcggcg gaatctttca gccgctcgat
     4681 gagcgcgtgc atccagcctt cggtcagagg cggctggccg caaacccatt ccaactccga
     4741 attgttgatc tgcgcctgca cggcggcgaa gtgcggcggg acgagtgtgc gaccgtcgaa
     4801 gccgatgagt gaatagagcg cgtcgaagcc gaagacgatg agcggcgaga ggtgcgtgcc
     4861 gtagagttcg cctttgaagc cggagccggg gaggccgccg acggtgctga gccagagcaa
     4921 ttgatcgggc ttgagcggcg tgccgtcttt ggcaaggagc atcttgccgt cggcatccat
     4981 gagcggcccc gagagcgcgg cgagcagtcg caccatctcg ttgcgcccgg cgcgtcccgg
     5041 cagagtcggc agcagattca acaactcggc caactcggcc tccgtcaaat tagcgagacg
     5101 acccgcgagc gtctccgcca gcgcgccctg ctgacccgcg cctttcgccc cgcccggaaa
     5161 ttgctgcgac gcgccgcgtt ccagtgcgtc gagtgtgtgc aggagttcga tcatttcgtt
     5221 gacgacgcgc gcgcgcccgg tcaactgaaa ctggctgtaa gagtcctgcg ccgggagaaa
     5281 cgcgccggag cgcagatcgc gccagagttc ggtcacctgt cggtcgcgct gttgagcgcg
     5341 ttggtggtcg ttatggagtt ctccatgcgc gttgaaaagc aggcggaaga cggcctcttc
     5401 cgcgcggcgc gcggcgtcgc cgccggtgcg ttcgagcctt gcgaaatgct tttcgagttg
     5461 gactgcgctg aagagttcgt ccgccgcctg atgaaccgtg cgccgcccgt gttcacccac
     5521 acgctccgaa gcgcgttcgg attgacgcag gaaatgatcg atcagctttt caactttatg
     5581 cccacgctcg ctcaacagtt cttcgagcgg tttttgtccg agcgcagtct ttaaagtgtc
     5641 gagcgcgccg cgcacattca caggcagcgc gccgttcccc gactcgccgc gcaggtactt
     5701 aaccgccgcc ggagttagag acgaatcatg gccgcgccca tgcacatcgc gcccatgcac
     5761 atcgcgcccg tgtgaatcgt gcgcgtgcgt ctggtttccc cgctcgacgc gtccgtgttg
     5821 atctccgttc cggtggtttc cgtgtgaacg attggagcgg gaattcgaat cgggatgtgt
     5881 ggttgaatcg gacttgggtt ggtggccgtt gccgcgcttg atcttgtctg cgacgtgctg
     5941 cgtctgcgct gccaactcgc cgacgcgtgc ggcgacgcgg ccattgcgtc gagattgccc
     6001 ttgcgactct ctgccgtgct cgtcgtgcgg attcgtcttg ccggagttgc cgtggacgcc
     6061 atcgccgtcg ccctgcgcgc cgcccgccgg atgctgcccc gtgccgtgta cacccggttt
     6121 gaaattgact ccgcccatca gagacacctc agaacccgcc cgtcaatttg tgcgcgacgg
     6181 tggaaggacg cgaagcatga caccatgtta gagagacgac gcccacaccg gatttcgctc
     6241 gccggataaa aacatcggac ggcgcgttta agccttctgg ccgctcaggt gtgagccgag
     6301 tgcgcgcgcg acgatggcgg tcgggtagtt tccgatctct ttaaacttcg ccgccgccag
     6361 tgtcgggaag taacgcgcgg aggcttgcgc cagcggcgaa tccgcgcccc ccgctttcag
     6421 gttctcccac ttcgaagcgt agctgcgcgc gaaactcact gcctctgcgc cgaccagccc
     6481 acgcgtccgc gccgacgcca gacccgcgcg gtaagcgtga cccatcgcca gcgcaaccgt
     6541 gccctcgccc ccgttgtaag ccgcaaggac aaacttccag aactccaccc cgttcggttg
     6601 gccgtaacgc gcgaacgcga tctggccgag ccgttgcgct ttgatgttca agagccgcgc
     6661 cgcaccgggg atagccttcg caggattcag gcgttcgtca gccgcgctgc ccgcgatccc
     6721 tacgcgcaag cccacctcgc gcgccgcctc gcgtccgaac tgcgctatgc cagcgtagcc
     6781 gtactggttg atgaccgtcg ggttgaaatt cgactcctgc gccagcaagc ccttcaagat
     6841 cgcgggcgac agacccggac accattcgac ggcgcgtgcg atcacgtcgt cgtaagcgcg
     6901 cgtcgaaccg ttcgcgagcc gcccttcgtt gattggcgaa tgggtggcgc gctcaaaggc
     6961 cgtctccatc ccgcgtgcgc cgcgcacatc gccgccgata acgttagctc ccagaaaatc
     7021 gccgacctcg ttgatcgcag ggtgaaccag aagcgcgctg atcgcgttcc actcgtcgag
     7081 caagggctgc tgatctttcg tcagcggttg accgatcatg tccgggtggc gcgcgtagaa
     7141 gatcaagtcg gtcaactgcc aggggtctgt cacgcccgac tgaatggcct gcaccagcgt
     7201 cttcaattct tcgagcgtgc gctgccgcgt ccacgcttca tcagacagat aactcgacgc
     7261 accgtttccg ccgtcgcgct cgcccgccgc gtggttatgt ttcggcgtga cggcctgagc
     7321 gtcagccgcc aacccccgtt caaagccctg ttcaaagttc gtgcgccggt tgcgaacaaa
     7381 gggcgtctcg cggcggacga gtttatcgcc tccatcgtcg ctcgccgggc gaccgctcct
     7441 gttgattccg tcaatgcccg ccatcgcttc cgctccccgc gcgcgcctga acttcgtcgc
     7501 cgcgcggact aaatcagacg ttcgtattga ggatggtttt gacgccgccc gtgacttgat
     7561 ccaccacctt actcatcact tcgacgtgct gcgaaacctg atagagccgc gcctgcaagc
     7621 cgagcaactc gccctgcgac agttccttgt cggacttcat gatcgattca atctggtgcc
     7681 actcgccctc gaccttggca aactggccgc gcaggtctgt gcccttcggc gtgttgcccg
     7741 cgcttccgat ggcttcacgc aacaggctga gccgcgtgcg cgagtcaatg aattcgggga
     7801 gcaacgcctt gacgttggaa gtatcggcgg gcagacgttc gatgcgctgt tgcagatcga
     7861 cgcgcatgcg ttcgaggtcg gcgcctgaaa cctgcgtggg ctgccctgcc ggattatgca
     7921 gatcgccgcc ctgcccgcct ccctcttgca gaacgctctc gaagctacgc gcgccctctt
     7981 tctgctgcgc gggttgttgc gccgcgccgc cgacctgctg cgaaatcgcc tgactcataa
     8041 tgccgaaaac tggatcgctc atcgttctct ctccttcgga actgttcgcg cggcggcggt
     8101 cgcttaatga aggatcgccg cgcgcgtgca attcgacctg atattccctc tcaatcaggt
     8161 tatcggcgat ttgcgaaata tgtttccgct gcggaggaga ttttttgaga tttttttcgg
     8221 gttacgcgcc cgcagtgccg cccgcgagtg tgcggagcct gaacagtagc gcgcgggcgc
     8281 gtgcgaggcg gagcgtctcg tcttcggatg ggaaggtttc gccgagccgc agggcggcct
     8341 ccaagtctct ggtggccgct gcgtactcgc cgagttggag gcaggcttcg ccgcgattga
     8401 cgagagcgga gatgttttgc gggtcggagg cgagcgcggc gtccaaatgg cctatggcga
     8461 gttgcggctc gccgaggcgc aggcgcagcg caccgagcgc ggcctgaaaa taggcggtcg
     8521 caggggctaa ggccgacagt ccctcgaaga tggtcagagc ttcctgctcg cgcccctgtt
     8581 cggccagcgc gtagccgagg tctgcgacca gacgcatctc ttcggtcgtc cactcgctcg
     8641 ccgccgcgag tgaagcgcgc ccttgcagcc aatcgttgag taagcgttcc gacatcgttt
     8701 cgcgcgtccc gttttttgct gagacgcaaa aataacacga cgcgcacgcc ccgcagcaca
     8761 ataaatcgcg cgccgcgcag gtcgcaccga aaaatttcaa aatttctcgt gccgccgagg
     8821 aaacaatttt ccgaaacggc tgataattat ggcgaaaggt caaaagcgac gggcggctct
     8881 ggaaagcccg acgacgggag gggcaaacga tgttcggatt gggcaaattg gtcggcggag
     8941 cgttaaacgc catcggactc ggcaagatcg cgccgttcat ctcgatgggc atcagcttct
     9001 tcacgggcga cttcgccagt ctggcgacgg acgtgatggg tctgatgtcc aacatcaaag
     9061 ggttggactt cctgaacaaa gtggcgcagt tcgccccgct cggcggcttc gaccagatgg
     9121 caggcggttg cttcggcgac ctcggcctga aacaactcct gaaggccgac ggcctgcgcg
     9181 acatcgccga caggttcttc aagatcgccg acaagctcga agacttcgaa gcctcctcgc
     9241 agaaggtgaa cgacctcttc gacctgctgc tcgataccgc gaagaaccgt gagtcgctgg
     9301 agaacgtgcg cagcagcgtc acgtacagca gttacatctc cgccgtctga ggtggaacgc
     9361 gagatgcttg agcggagtga atttttgatg acacgccagc aatcgaatgg taaacagaca
     9421 cccggcggcg acgccgcact cccgacgctg cgcgagcgtc tcggcatcag cgaggaacag
     9481 tttcgggaga tgggacaggt cggggcgatg tattacaagc agggcaatct ggagaaggcg
     9541 cgcacgattt ttgaaggctt ggtcgaagtt gacccgcaga gcgtggacgc acactccgca
     9601 ctcggcgcgc tctacacgcg cacgcagcgc gacgcggaag cgttgcatca cctgaatcgc
     9661 gcgctcgaac tcgacgaccg gcagatcgcc gcgcacgtca atcgcgccga aatttacctg
     9721 cgccgccaac aggttgaaga ggcggtcgcc gacttgaaga aggcgattga acttgatccg
     9781 catgaacagg acgcgggagc caaccgcgca cgggcgatgg tcttaggcat ccacgaggct
     9841 ctggaagcca aaggtgtgat gtagggcacg aaacaagcga ttactaaaaa cggaacagag
     9901 gagaacagat catgagtaac tcgattcaag gcttcaacat tcccgcaaaa ccgaatccgc
     9961 aggacttcga cttcacagac ccgcagcagg ccgccgcgta tcaggagaaa tcgcaaaact
    10021 atcacttcgc ggttgacatg atgatgaacg ccatcaagca ggaaggcgag gcgaagtcga
    10081 acctgctcaa gacgatcaac gacgggctgg acgcgcatat cagaaatctt ggctaatcgc
    10141 gcgcccgtcc ccttgcggtt ggcataattt atacggaaac ggtgcacgcc ccacgggcgg
    10201 cgcatcgttt ccgtatgtta gtttgctgct ccggcaaacg cccgtcgtgc aactcccggc
    10261 ggcgcgtcgc tcatcattaa gtcgggcgct ggttaacttt ccccttccgg tttatcatca
    10321 acctctacga acgcgagttc acttcccccg cgagttgact acaggcatcc gaggtaacga
    10381 catgacgaaa cttatgcgag ccatcacgat cagttgcgcg ctcgtcgtgc tgctggcggg
    10441 cagtgcgttg gcgcaatacg gcggcaggta tcgcggcggc ggcggcacga cttaccgcag
    10501 gccgtccacc gccacgtcga gccttattct gcgccgcagc atccagcgcg cgcaggtgcg
    10561 acgccagaaa gcacgcgcgc tcaaacgtcg ccaggccaac gcggcgaacc ggcgtcacac
    10621 gcagcactag aggccgcgac cggaggaggt ggcgagatga gattctacga gcaacgcctc
    10681 gcgcgacgcg cgctgttacc ttttctcctg ctctgtctgg ccgcctccgt cgcgtctgca
    10741 cagacctaca gccggggcac ttacagcggc gcgaattcca cacgctggaa caacccgatg
    10801 tccgcgagcc tcaatcgcat gctcaaccgg aacattctga accagagcat tcgacggcga
    10861 cattcaatgg gcagcgcacg cgccacgagc ggcaataacg ctccccgcac gaccaacacc
    10921 cgcacaactc caaacggcac ggtcaagccc gtcaacagca cgcccgcgaa caatagcgcg
    10981 actgcgtttc gtcccgtcgc cagttcgctc tacccgcaac agttggcgcg tgaagcggca
    11041 acggacgagg ccgagcgcaa ggagatggaa gccgtctttg cgatgtgcct gcggaattac
    11101 gaagcgaaca tgcgtcgtca gggtcagccg ttgaaagacg ttgcgcgcgc cgtcagttac
    11161 ttcatcggcg tcaattacaa cgtgctgcac ggcggcggca cggtgacgcc cgcgcaaggc
    11221 gtcgccctcc gcgcaggtat ccaggccgcg ctcggcgaag atgaaagttt ccggcggctc
    11281 tcggatcgag agaagcagca gatttacgag acgatggccg tgctcgccga gttcgtcgcg
    11341 ctcaacatca gcgtggccga cgagcgtggc aacaaacaac tggccgaagc gacgcgcagc
    11401 atggcagccg acaatctcga aaaactgctc ggcgcatcag ccgcgagcat ccgcatcacg
    11461 gatcgcggga tagagtttta gcgcgagtca caacgtaacg acggtacaac ggaatgatgg
    11521 aagggcggcc aacgcgaact ggccgccctt ttcatttgtg ccgttcgcgc cgtttcaaac
    11581 gctcgcgagt tgacgacgcg ccctatccgc cgccgatagt cttgagcacg gcctcgaacg
    11641 ccctgtaggc ttccttgaaa tagtagatat atcgttcgcg gtagagcggg agcgcggcga
    11701 ggacgacgac gaggccgacg gccatcttca ccggcaaact caggaagaag acgttgatct
    11761 gcggcgcgac gcgattgacg aggccgaaga agaggtctgt cagtaagagc gcgatcatgg
    11821 caggggcggc gagttgaatg ccgacggcga gcacgccgcc ggtcagtttc gtaatgaagg
    11881 cggcggcggg cgaccagccc gcttgcaggt gcgggaaact catcgccggg atgatctcga
    11941 agctgcgcag gagcgatgtg atgaaggcgt ggtgcgcgcc gatcatgaag aagaggacga
    12001 tggcgagttg cagtttgaac tggccgagtt ctgaaacgcg cgtctgtagt tgcggcgcga
    12061 acatctcggc gagcgtcgag ccgcgctgga agtctatgac gcgccccgcc acctgaatcg
    12121 cctcgaagac gagcgaggcc acgaagccca gcgtgaagcc gacaaacgcc tccttcacga
    12181 gcagccctat gaagccgacg gggccgaagc cgagcggcgc accgtccttc ggcagtcccg
    12241 cttcgagcga cggatagagg atgatgacga aggccgtcgc cgtcgctacc ttgacgcgcg
    12301 cgggcacagc cgcgccgccg aagaacggca cgaccgtgag gaacgacagc atccgcgcga
    12361 acgccagccc gatcagtacc agaaaagact gcggactctg cccgacctcg aacgtgcgca
    12421 ggatcgtctc taagagtttg agaaactcgt ccataaagaa acgatgaagt aggaaggatg
    12481 aacgatgaac gggagacaga attcagaagt cagaatacgg gagtcagaat aaaacgcgcg
    12541 ccgctcttcc ttctgactcc tgtattctga cttctgaatt ctccttcatc gttacttgct
    12601 acatgccctt gcccacgtag ggcatcatgg cgaaaacttt gtggccgaag atgagcaggc
    12661 ggtcgagcat ccagttcgcg ctgatcatca ggacgagcag cgtggcgatg atcttcggca
    12721 cgaaagtcag ggtctgttcc tgcacctgcg tcaatgcctg tagcaggctg atgacgaggc
    12781 cgacgacgag gctcgcgatg agcggcggca gagagagcaa caggatcagc cacagtgctt
    12841 ctgcggcaag cgagacgtaa gttcccatcg aagttcacct ccggcagacg tgcgttcagg
    12901 tatagctgag cacgaggttc tgaatcagca gcacccagcc gtcaattaag acgaacagca
    12961 gcagcttgaa cggcagcgag atcatgacgg gcgagagcat gaacatgccc atcgcttgca
    13021 ggatattcgc caccaccata tcgatgatga gaaacgggat gaacagcagg aagccgatct
    13081 ggaacgcctc gctcaactgg ctggtgacaa acgccgggat gaccgtgcgg aagtcgctga
    13141 cgttgatctc gttcgggtcg ttgccgttct tgacggccat ccgtcgcgcc agatcgaaga
    13201 acattttgcg gtcgcgttcg tgcgcgtgac gttcgaggaa ggcgcgcagc ggctcgcgcc
    13261 cgcgttgcgc cgcctcgaag agcgtgcgca cggagacttc cgagaagatc gcctgtgagt
    13321 cgccgatgtt ccccgccgcc gcgtacatct tctcgaccac gggcgacatg atgaagatcg
    13381 tcaggatcag agagatgccc gtgataactt gattgggcgg cacctgctgc gtgccgagcg
    13441 cgttgcgcag gatggagagc accaccgaaa ttttcacgaa cgacgtgagc atgatcagcg
    13501 cgaacggcgc gagcgcgagc gcaccgagca cgatgacgag caccaccgga ttcgccgtcc
    13561 cgccgtctct ggcaggcacc tgcgcctgcg cgtgcgcgtc cacggttgta aacgcggcga
    13621 gcgcacacat tgcgacgaag ccaatcaaag tgattccggc cagtttcttc cagacggcag
    13681 gcttggtgtt catggctcac ttcctcttgc cgagcagacg tgagaagcgt tcggccaccg
    13741 cgtcgcgcgc cgtcgcggtg gactgtttga agttcgggcg caggcgttcg acttcgtgcg
    13801 cggcctcttc cgcactcgtc gcgtccaact cactgatcaa ctgcacgccc gcctccgacg
    13861 aagcgatgag cagccaacgc cccgccacct ctatgacgta gagactcttg cgcgtgtcca
    13921 gccccgtgcg gtctacgatt cgcaccatgc tgcccgacgc gccacccacc tgttgcaagc
    13981 gcggcagcac ccagcgaaag agcacgtagg ccagtccgca gacgagcgcg agcgcgacaa
    14041 tcgtctgcgc gagcatggcg aagaaactcg cgccgctgtc gttgtactgc gccgggtcga
    14101 acggcgtcgg ggcttgcagc gcggcaagca cagacgcgca aacgccgtgc agcaggccgg
    14161 gcgcgtccat caacgcaaca cctgcgtgac gcgcacgccg aggcggcctt ctatttcgac
    14221 caactcgccg cgcgcgacgc gacggccatc ggccaccaga tcaaccgggt cggttgcctg
    14281 acagccgagc ggcaacaact ggccgacgcg caggcgcgcc aactcgtcga ggctcaaacg
    14341 tcgttcggcc aattcgacgc gcaccgtcag caaaatctct tcgagcgcaa tcgcgctctc
    14401 gttcgagtct tcgtcgctgg tctgctcttg ttccatgtcc catcgctccg tgccgtgaag
    14461 ttccccgccc cgcttgatct gctcgattaa cagttgcgcc gcatcatcgt caggcatcgt
    14521 caacgtgccg gtcaatatcg cgcccgcgcc gtcgccgaga cacatctcca aacgacccga
    14581 cagacgcccc gcttcgaacc gccccagagg tcgcgcgacg acgacgacat cgccggactc
    14641 caaatcggcc agatcaatcg ccctcacgtc cgtctcgccc aacacaggcc ggagtcgaac
    14701 gtcgggcgcg acgcgtttga acagttccct gcgcgcgtcg cgttcgcgcc cggcgaagag
    14761 cggtgaatgg cgcaagcctt cgagcgcagc gagcgcgtcg cgcgtgaagt agaagctcac
    14821 cagcccgctc agcgcgccga cccgcaaagc gaacgtcgcc acgatggcgc gcgcgcccgt
    14881 ctgttccttc gtcgcgttgc ccggcgcgcg cgtgttggac tgcacgccgg gtcctccctt
    14941 gagcgacgcg ggcggctccg tcgaaacgcc ttcgagacgc cagagcggcg cgtccacctg
    15001 ccgatgcaat tcacggatca ggcagagcca gagaaactca aacgcggcca gttccgtacg
    15061 cgaaagcagg cgcaaggttt cgggcgcgtg cccttcgccg cccaacatgc ggtctaccag
    15121 cgcagaggca aagtcggtct gcaaggaggc gaagaggctg gcgtttgcgg gcgcgagtgc
    15181 gagcgtcgtc cacgcgtcca cagtcccgcc tgttgacgta gagttggagg agaactcccg
    15241 ctctcgcacg tctgcctgcg agacgacgat ctcatcctcc ggctcgcgca ggtattgcga
    15301 caggacgcgc acgagcgtcg cccgcactcc ggcggaaagg gtcggcagag acgcgccgag
    15361 cgcactcgat agacgcaact ctgcgcgcga gacgcgtggc aggcgaatcg accacgccgc
    15421 gcgcgcatcc gtgtcggcaa acaggtcggc catggcgtcc ggcgcggcga cgtgcgcggg
    15481 tgcgcccgcg ctatgcctcc cgcgcgcgaa cagatcgacg gcaaaaggat cgccgacctc
    15541 cgtgccttca cgcacggtcg gtgcctcggt tccggccttg tccgtcgcat cagctttcat
    15601 ggcagaacgt ctgaacagtg cagggcgtgt aaaagagaga tcgctggtac tattttattc
    15661 gccgtccgtc tacgaatttt caagcgcgat aactagaagt gctttcgtct gcggcgcgct
    15721 cgtcctgcga cgcatcttcc gctgccgcag acgcgtgcgc gccatctctg ccggagagga
    15781 cgcgcggcgg gttgtcaggc gcgtgacgcc cgccgtcgcc gctcgtcgtc tgatctccgt
    15841 tcgcgccgac ggtcgtgcgc agcgacgcga gttcgacgcc gcgcccgcgc aggagatcgg
    15901 agaggtcgcc cgcgcgcgcg tcgagttggg cgcgcaattt ttcgctcgcg gtgatgaact
    15961 ccgcattgac acgcccctgc ccgtcagtcg tcaggcgcac gcgcaagcct tcgagcacag
    16021 agcggtggag ttcgagcgtc acttcctgtc gcccgccgga gatcaactgt gtgcggacgg
    16081 cggcgacgat gcgttcgaga tcggcgatgt gcaggatggc gcgcgcgggc gcggcctcgg
    16141 agatgtgtcc ggcatcgcgc acctgcccgc gctgctcgaa gccgccccca ccggtgccgc
    16201 cgtcgtgccg gtcttcgcgc cgcttcgact cgcgctcgcg ttcggcgtgc gcggagcttt
    16261 ttgtctcctg ccgctcgcct tcactgcttt cgttgtcgtc gcgctcggca gtgcgcgtta
    16321 catcttcgag cacgctggcg aagtcgcgtg tggtgtggac ggaggcgagc gcgccatcgt
    16381 cctcatgcgg cacggtgttc gtctgcgagg agacgcgtcg agacgtgatg ttgttcgtcg
    16441 tggggggcgg ctccggcgtg ttttttggtg gcgtgacttt cattgactcg ctcctttcaa
    16501 gactacagcc gttgcggcat cctgcactga cccgcgccct tacttcatct tgcgcaactg
    16561 ctctgcggcc tggttcgcca tttcgcgtgc gcgtttgcgg ttgatggtga cgccggtttg
    16621 atctaacgcg agggcgcgct ccagtgcctc catagtcgcc gcgaactcgc gcagttcaaa
    16681 catcttctcg ccgtagcggt aggcggcaag tgcggacttc ggatcgagtt ccgtcgcctt
    16741 ctgataagcc tgcgacgcgc tgagcggctg cttcatcccg tcgtaacagt cgccgatgta
    16801 gatgtaaata tcggctgacg gttgcgtctt ggagatttcc agcaactccc tgagcgcggc
    16861 agaccattgc ttgcgcttca tcaacaggcg cgtcgaatcg aggctggatg aagtgggcat
    16921 gggtccgagc cttttcgcgc gcgcgtaata agagttcgcc tccttgttct tgccctgctt
    16981 ggtgaaggcg tcgccgagtc ctttgtaaac gagcgcgagt tcctgattca actcggcggc
    17041 gcgctggtag gctctgacgg cctgcgcggg ttgattggtg gcgagatagg cgttgccgag
    17101 actgtagtgg gcggcgacca actccggctc cgccagcact gccttgttga aggcgacgag
    17161 cgcgcccgga tagtcgccgc gcaggtatgc gagttgcccc tgcgcgaaga gagcgcgcgc
    17221 cctgagttgc ttgttcgcgg ggtctttctc cgacgcggaa gtgttgtagg tttcgacgat
    17281 ttgccagtcg ctcgcggaga cgttggcggt ctgctgcgcg tcgaggtgac gcttgatgat
    17341 ctcctcgaca gcgttggcgg cggattcggc ctctttcgct gcggtgtcat cggcgggcgc
    17401 gggcgtcgcg tcggtcttcg cctcttcggg actggccttc ggagtgggca gtgcgacgtt
    17461 gaagctgaaa ttggtgttgc cggggcgcac gtcaatctgt tgccgctcgg tgcgctggcc
    17521 gatgtgtgag acggtgacgc tgtgcgaacc gcgcggaagt ttgtaggaga gtttgccgtc
    17581 ggcgtcggtc ttgccgagct tctgcggcgt gccgccgggt gagttgagaa agatgtccga
    17641 gtccggcatg tcggttgtga agacgacttc gagcagggcg gcggcgggcg cgggacgctg
    17701 gcggcgacgg ttgcccttgc gtgtcgcgtc cgtctgcttg ttggcgttct ccttgctcgc
    17761 gccgccgttc tttttctccg tgctcgcggc gtcgtcggcg atgctcggtt tcttttgcga
    17821 cacggccacg tgttgagaac cgagcaagac taacagcaac cctgcgcata tcgctgcgcg
    17881 cattatgaga gacgtgctgg tcgaaccttc gatagaattt ttccggtaca tgtttgtcat
    17941 ctattcaaat gctccgtcaa cttaacattc aactcactaa gcctgtcccg cgtcagttca
    18001 cccgccgcgc tttcgacgcc gcatcgtcag ccatcttgcg cgtgcgtgcg cggttgatgg
    18061 tcgttcccgc ctgatcgagc gcgagcgcgc gctccaacgc ctccgccgct cccgcgtact
    18121 cgcgcgaccc gtacatcatc tcgccgtagc gtgcgtgcgc ctgcgccgaa ccggaatcga
    18181 gttcgatagc gcggcgatag gcgggtgcgg cgctgagcgg ctgtttcagc tgcatgtaac
    18241 agtcgccgag cgcgatgaag acttccgcag agggctgcac cttcgacaag tcggaaagtt
    18301 cacgaatggc ctccgcccaa cgcccgcgcc gcatcaggtt gcgccccgtg ctcagactcg
    18361 cgctggcgga aaagtagccg agcgtttttg cgcgctcgta ataggtgagc gcgtccttgc
    18421 tcttgccctg ccgcgtgagc acgtcgccga cgccccggtg agccatcgcc agttggccgt
    18481 ccaactcaat cgcacgctgg taagcacgca gggcttcggg caattggttg gtggcgaagt
    18541 aggcattgcc caagccgtag taagcgagcg cggaggtggg cagcgcgagc gcggagttgt
    18601 taaaggcgac gagcgcgccc gcgtagtcgc gccgcaggta agccaactgc ccctgcgcga
    18661 agagcgactg cgcgcggact tgcgcatcgc ccggattcgc tgcgaaggcg agggcggtct
    18721 gttgctgcgc cgcctcccat tcagcgaccg tcacgcggtc ggtctgtttc gggtcggaga
    18781 aacgcttgag cacttcggcg gcggtcggga gcgtgccggg cagcggttgc ccggacaggt
    18841 tgaggatgaa gtttgcgctg cccggctgca cgctgatctg ttgtcgttgc gcgttgtaac
    18901 cggcgcggct ggccgtgacg ttgtagacgc cgcgcggcat gcgtgtgcga agtttgccgt
    18961 ccgctcccgt cttgccgagg ctctgaagtt tgccgcccgg cgcatgcacg aagatgtccg
    19021 actcggccac gcccgtccag aaggtcacat cgagcagcgc ggacgggcgc ggcacggcgg
    19081 gcgaagggtt gcgtcgcggc gtcggacgcg ttgaggcggc gcgcttgccg ccgttcgtgg
    19141 cgtcggcctt cggcttcttg ccggagtttt tcacgtcgcc cgcgatgctc ggcctgtctt
    19201 gtggcgcggc cagcgcgttc gtcaagcaca gaagagacgt tagcaacgcc gcgcaccaag
    19261 cgacgcgtag cgcacttact agaattttga actgctgcac accc
//
LOCUS       DASWNS010000480         6400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_147663, whole genome shotgun sequence.
ACCESSION   DASWNS010000480 DASWNS010000000
VERSION     DASWNS010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147663"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2099
                     /locus_tag="VGB76_16560"
     CDS             <1..2099
                     /locus_tag="VGB76_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0100560.1"
                     /translation="FVALFLSPFISRAADFRREVVYQIITDRFFDGNSANNNPAQSAG
                     LYDATKANWRAYWGGDLQGIQQKMSYLAGMGVTAIWISPPVDNLNANIPDGNGNPTAS
                     YHGYQGRDFKRIEEHSGSASNAWTDFDNLVSAAHANGIKVIVDFAPNHSTQDNAGEFG
                     ALYDNGTFLGNYTNDTNGLFHHNGNISNWDDRYQVQYYTLFNLADLNQEHATVDAYMK
                     ATAQLFQQHGVDGFRIDAIKHITWGWQYSLANAIYTNGDSFLFGEWYQTSTSDPLYRD
                     SYKYTNKSGISMLDFPLNTAIRNVFGSNANFSEIDAVLTAEAANFTWDEDLVTFVDNH
                     DMTRFLSLLNNNNRLHQSLAFILTQRGIPCIYYGTEQYLHNDTSGGGDPYNRPMMNAF
                     NTSTTAYTLINRLSTLRRNNPAIGYGTTQQRWMNNDVYIYERKFYGNTVLVAINKNET
                     SSYQITGLNTSLPVGTYSDHLTGLLGGSNISVAAGTGGNNPVANFTLPAHTVAVWSFT
                     AAPAAPQIGVIGPTVAQPGVKVTIGGRNFGTTTGTVKFGTTTATVNSWTSTKIVATVP
                     VVTNGNYNVTVTNSSAQVSNGIQFTALAAKLIPVTFTVFNASPTQTGDYIFLTGNTIE
                     LSNWATTWDAAVGPMLTPNYPNWFLNVSVPAGKTIQFKFIKIAANGAVTWEAGANHSY
                     TVPSSGVGFVNVNWQY"
     gene            2227..2922
                     /locus_tag="VGB76_16565"
     CDS             2227..2922
                     /locus_tag="VGB76_16565"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100561.1"
                     /translation="MKKFFILAGLLVTPVLHMQGATGARQQAQTSPAVDVNVKAKPGA
                     RPKIDPAAWKKYAGRYELGAGVIPISTLDVTFENNELWVKPSLVKKRRLVARSKRVFL
                     DEIEGTRYTFDFDEEGRVVSLTFRFEGSAYTARKVALPPPSVKGNTTFKLQGYADAQV
                     VVLAGSFNNWNESQLLFAREGDAWVCRVDLAPDRYTYKFIVDGNWMLDPANAETEEDE
                     YGNLNSVLVIKQK"
     gene            complement(3322..3579)
                     /locus_tag="VGB76_16570"
     CDS             complement(3322..3579)
                     /locus_tag="VGB76_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100562.1"
                     /translation="MNTSRKLTISLILSQVITSGCAGTKLPQLVEAKPKRFAELVPAS
                     PLPPDQIEGEPDTLTLVVNIDMEYRLTLNLESMGTASNTRQ"
     gene            complement(3746..4378)
                     /locus_tag="VGB76_16575"
     CDS             complement(3746..4378)
                     /locus_tag="VGB76_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100563.1"
                     /translation="MASETATHPSESDRHIFDTKATPRQVELIPEHGMSRRAQTALYV
                     ATVLVFVLAAAAVVMMWHSMQALRSEVARQSDEIKVELEKFKLEVIEKREAEHKATLD
                     AMNKERARREEEYQKATSAAINKAIAKDREEYQKVNSEEIVEAARKAASEEVAKAFPN
                     NRNGVRNRDGGRATETASSGRKPVGKAWQEQARANSRRSRDRRRADGHGR"
     gene            4995..6332
                     /locus_tag="VGB76_16580"
     CDS             4995..6332
                     /locus_tag="VGB76_16580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY0100564.1"
                     /translation="MIKDELMELIGARRAHVGVIGLGYVGLPLSVEFAHKGFQATGFE
                     VDAEKAAQINRGESYIGDVRSTLVAELVAAKRLRATTDFAELSKCDCVIICVPTPLRK
                     TKEPDISYILAAAGEIQKHQRRGQLIILESTTYPGTTDEVLLPMLEEHGMKLDEDFLL
                     AFSPERVDPGNPQFQTHNIPKVVGGSTDDSTEVAAALYGQIVENVHPVTSARVAEAAK
                     LLENTFRAVNIGMANEMARLCYALGIDTWEVIRAAATKPFGFMPFYPGPGIGGHCIPL
                     DPHYLSWKARQHGFDSRFIGLAEEVNSRMPDHVVTLVADALNDERKALKGSRILLLGV
                     AYKKDVDDVRESPALSIIDRLRAKGAEVSYHDEYVSEIRFDDAHTESAGEPLQSIALT
                     DDELKAADCVLIVTDHSAVDYARVCERSTLIVDTRNALNGDLRRKSCARIIRL"
BASE COUNT         1314 a         2035 c         1752 g         1299 t
ORIGIN      
        1 tcttcgtcgc actcttcctc tcgcccttca tttcgcgcgc cgcagatttc cggcgcgaag
       61 tcgtctatca gatcatcacg gatcgcttct tcgacggcaa ctccgccaac aacaacccgg
      121 cacagagcgc gggactctac gacgcaacga aagcgaactg gcgcgcctac tggggcggcg
      181 acctgcaagg catccagcag aagatgtcct atctggcggg gatgggcgtc accgccatct
      241 ggatttcgcc gcccgtcgac aacttgaacg ccaacatccc ggacggcaac ggcaacccga
      301 ccgcctcata tcacggctat caggggcgcg acttcaaacg catcgaggag cattccggca
      361 gcgcgtcgaa cgcctggacg gacttcgata atctcgtctc tgccgcacac gccaacggca
      421 tcaaggtcat cgtggatttc gcgcccaacc attcgacgca ggacaacgcg ggcgagttcg
      481 gcgcgctcta cgacaacggc accttcctcg gcaactacac caacgacacc aacggcctct
      541 ttcatcacaa cggcaacatc tccaactggg acgaccgcta tcaggtgcag tactacacgc
      601 tcttcaacct cgccgacctc aatcaggagc acgcgaccgt cgatgcttac atgaaagcca
      661 ccgctcagtt gttccagcag cacggcgtcg acggcttccg catcgacgcg atcaagcaca
      721 tcacctgggg ctggcagtac agcctcgcta acgccatcta cacgaacggc gacagcttcc
      781 tcttcggcga gtggtaccag accagcacta gtgatccgct ctaccgcgac tcctacaagt
      841 acaccaacaa gagcggcatc tccatgctcg acttcccgct caacacggcg atccgcaacg
      901 tcttcggctc aaacgccaac ttctccgaaa tagacgccgt gctcacggct gaagccgcga
      961 attttacgtg ggacgaagac ctcgtcacgt tcgtagacaa ccacgacatg acgcgcttcc
     1021 tctcgctcct gaacaacaac aaccgcctcc accaatcgct cgccttcatc ctcacgcagc
     1081 gcggcatccc ctgtatctac tacggcacgg agcaatacct gcacaacgac acctccggcg
     1141 gcggcgaccc ctacaaccgc ccgatgatga acgctttcaa cacctcgacc accgcctaca
     1201 cgctcatcaa ccgtctctcc accctgcgcc gcaacaaccc ggccatcggc tatggcacga
     1261 cgcagcagcg ttggatgaac aacgatgtct atatctacga gcgcaagttc tacggcaaca
     1321 ccgtcctcgt cgccatcaac aaaaacgaga cgagttcata ccagatcacc ggactcaaca
     1381 cctcgctccc cgtcggcacc tattccgatc acctgacggg cttgctcggc ggctccaaca
     1441 tcagcgtcgc cgcagggacg ggcggcaata atcccgtcgc caatttcacg cttccggcgc
     1501 acacggtcgc cgtctggagt ttcaccgccg cgcccgccgc gccgcagatc ggagtgattg
     1561 gtcccacggt cgcgcagccc ggcgtgaaag tcaccatcgg cggcagaaac ttcggcacga
     1621 ccacgggcac agtcaagttc ggcacgacta ctgcgaccgt caactcctgg acttcgacga
     1681 agatcgtcgc caccgtcccc gtcgtcacaa acggcaacta caacgtgacc gtcaccaact
     1741 cgtcggcgca agtctcgaac ggcatccagt tcaccgcgct cgcggcgaaa ttgatccccg
     1801 tcacgttcac ggtcttcaac gcctcgccga cgcagacggg cgattacatc ttcctgacgg
     1861 gcaacacgat tgaactgagc aactgggcga cgacgtggga cgccgccgtg gggccgatgc
     1921 tcacgccgaa ttatccgaac tggttcctga acgtgagcgt gcccgccgga aagaccatcc
     1981 agttcaagtt catcaagatc gccgccaacg gcgcggtcac ttgggaagcg ggcgcgaatc
     2041 atagttacac cgtgccgtcg agcggcgtcg ggttcgtcaa cgtcaactgg cagtattagt
     2101 ggtaagggag aagggaaggg ggcgagggga gagggcaagg gccagacaat gatctacgcc
     2161 gaccgctcta tcttttaaac cctctccctg tatcccttcg cctttccttc tttcgaggtc
     2221 ggcgcgatga agaagttttt cattctcgcg gggttgctgg tgacgccggt tcttcacatg
     2281 cagggcgcga cgggcgcgag gcaacaggcg caaacgtctc cggcagttga cgtgaacgtt
     2341 aaggcgaagc ccggcgcgcg gccaaagatt gacccggcgg cctggaaaaa gtatgccggg
     2401 agatatgaac tcggggcggg cgtcattccc atctccacgc tcgacgtgac gtttgagaac
     2461 aacgaactct gggtcaagcc gtcgctcgtc aaaaaacgca ggctcgtcgc gcgctcgaaa
     2521 agggtcttcc tcgacgagat cgaaggcacg cgttacacgt tcgattttga cgaagagggg
     2581 cgggtggtaa gtctcacctt tcgcttcgag ggcagcgcct acacggcgcg gaaggttgcg
     2641 ctgccgccgc cgagtgtgaa aggaaatacg accttcaagt tgcagggcta cgctgatgca
     2701 caggtcgtcg tgctggcggg ttcgttcaac aattggaacg aatcgcagtt gctctttgcg
     2761 cgcgaaggcg acgcatgggt ttgtcgcgtc gatctcgcgc ccgaccgtta cacttacaaa
     2821 ttcatcgtcg acggcaactg gatgctcgac ccggccaacg ccgagacgga agaagacgag
     2881 tacggcaatc tcaactcggt gctcgtgatc aaacagaagt agaacccgac cccgaaaatt
     2941 ttcaccgctc cgagcagttg cgtgtagagg ttgccgtaag tgatgcgcgc ggtgcccgca
     3001 tccaacgtct cgttgactgt tcgcggcagc ggcaggtgcg acacctgacg cctaaacctg
     3061 tcgagctagc ttatcaactc tcgcggatca aggcgtccgg gcgtcgtcgc gtcgttaacg
     3121 ctaattttcc gtgaggtcgt ctatttgtag gccgatggaa ttgctgcccg cagacttgat
     3181 agcctcgatc actttaacca cctcaacgta gcgcgcagtg cgaggagctt taacaaacac
     3241 ggctttcgca atcttgtccg aaccgtgttc atagacgcga ttacgttcgc gctccgcaaa
     3301 aatcttcttc agacgtttgc gtcactgtct ggtattgctg gctgtgccca tagattccag
     3361 attgagggtt agtctgtatt ccatgtcaat atttaccacg agcgtcagcg tgtccggctc
     3421 gccctctatt tgatccggcg gcagaggact ggccggtact aattccgcga accgcttcgg
     3481 tttcgcctcg acgagttgcg gcagctttgt cccggcacac ccggacgtga tcacctgcga
     3541 aaggatgagt gagattgtca gtttgcgtga ggtgttcatg ggttttcctt gaagacagtt
     3601 gaggccgtca gacctgaaag aatttcggag cggaatcgcc cgctcgctgc atttctaaac
     3661 tcaatgcagc gagcgggcga ttccgctccg ttagcctgtg agcgcggcaa gctttccgct
     3721 ctattttgtc ccgcgaccgt agaggttatc gtccgtgtcc atcggcacgc ctgcgatccc
     3781 gcgatcgccg tgagttggcg cgagcctgtt cctgccacgc cttgccgaca ggcttcctgc
     3841 cactgctggc cgtctccgta gcccttccgc catcgcggtt gcgcactcca tttctattgt
     3901 tgggaaacgc cttggcgacc tcttccgagg cggccttgcg cgctgcctcc acgatctctt
     3961 cactgttgac tttctgatac tcctctctat cctttgcaat cgccttattg atcgctgccg
     4021 aggtggcttt ttgatattcc tcctctcgcc tggctctctc cttattcatc gcgtcaagag
     4081 tggccttgtg ctcggcctcc ctcttttcga taacctccag cttaaacttt tccagttcga
     4141 ccttaatctc gtcagactgt ctggcgacct ccgaccgcag tgcctgcatg ctgtgccaca
     4201 tcatgacgac ggctgccgct gccagaacaa acaccagcac ggtcgcaacg tacaacgcgg
     4261 tctgcgcccg ccgactcatg ccgtgctccg ggatgagttc aacctgtctc ggggtggcct
     4321 tggtgtcgaa tatatgtctg tcagactccg acgggtgggt ggccgtttcg ctcgccactc
     4381 cctgatcggc aactggcgcg taattctctt gcgcgccgtt aatggaattg ccgcactttc
     4441 ggcaaaagag cgcgtcatcc tcgtgtgtcg agtcgcaggc agtgcatcgc ttgatagact
     4501 tcccgcacct tcgacagaag agcgcgtccg gtttgttttc aacgtcgcag tttgaacatc
     4561 tcatgggggc tacatccttc cggttgaggt cttttacagg gataggccat tcagtagacg
     4621 gcgcggcgcg aggtgccgtc cggggagagg tttaatggcg aactgaactt acgccgggct
     4681 ctgatcacag ccttttgtgg cctgagtata gggagatagt tcgaggccgt caatctttat
     4741 gtttgccggg gaatggctaa atcggcgcag gctgcaagtg aaacgcgatt cttcgcgcgt
     4801 cctgcggctg gcagcagata tgcctgcggt cgaaactgga aatcttaaac acacgcgggg
     4861 atttagtcgc cccgcgaatt tcgatgcgct tgacagcctg cgtgcggctc acctagtctt
     4921 gatcgttagt aattcataaa cttcattatc tcctgccgag tgatttagac acgggtacgc
     4981 gaaaaaaatt tcctatgatc aaagatgaac tgatggagtt aatcggtgcg cggcgcgcgc
     5041 atgtcggcgt gatcggattg gggtatgtgg gcttgccgct ctcggtcgaa tttgcgcaca
     5101 aagggtttca ggcgacgggc ttcgaggttg acgcggagaa ggccgcgcag atcaatcgcg
     5161 gcgagtctta catcggcgac gtgcgctcca cgctcgtcgc ggaactcgtc gcggccaaac
     5221 gcctgcgcgc gacgacggac ttcgccgagt tgtcgaagtg cgactgcgtg atcatctgcg
     5281 tgccgacgcc tctgcgcaag accaaagagc cggacatctc ctacatcctc gcggcggcag
     5341 gcgagataca gaagcaccag cggcgcggcc aactcatcat cctcgaatca accacctatc
     5401 ccggcacgac cgacgaggtg ctgctcccga tgctcgaaga gcacggcatg aaactcgacg
     5461 aggatttcct gctcgccttc tcgcccgaac gcgtcgatcc cggcaacccg cagtttcaga
     5521 cgcacaacat cccgaaagtc gtcggcggct ccacggacga ttcgacggaa gttgccgccg
     5581 ccctctacgg ccagatcgtg gagaacgtcc atccggttac ctctgcgcgc gtggccgagg
     5641 cggccaaact tttggagaac acgtttcgcg ccgtcaacat cggcatggcg aacgagatgg
     5701 cgcgcctctg ctacgcgctc ggcatagaca cctgggaggt gatccgcgcg gcggcgacga
     5761 agcctttcgg cttcatgccc ttctaccccg gtcccggcat cggcggacat tgcatcccgc
     5821 tcgacccgca ttatctctcc tggaaagcgc gccagcacgg tttcgattcg cgcttcatcg
     5881 ggctggccga agaggtcaac tcgcggatgc ccgaccatgt cgtcacgctc gtcgccgacg
     5941 cgctgaacga cgaacgcaag gcgctcaaag gttcgcgcat actgctgctc ggcgtggcct
     6001 acaagaagga cgttgacgac gtgcgcgaaa gccccgcgct ctccatcatc gaccgcctgc
     6061 gggcgaaggg cgcagaggtc agttatcacg acgaatacgt ctccgagatt cgcttcgacg
     6121 acgcgcacac agagagcgcg ggcgaaccgc tccaatccat cgcgctcaca gacgacgaac
     6181 tcaaagccgc cgactgcgtg ctcatcgtca cagaccacag cgcagttgac tacgcacgcg
     6241 tctgcgaacg ctccacgctc atcgtcgata cgcgcaacgc cctcaacggc gacctgcgcc
     6301 ggaagagttg cgcgcgcatc attcgtttat aaaccgtcgc aagttacaag tcccaagtcc
     6361 caagtctgaa cacctgctga cttgcggttt gggacttgtg
//
LOCUS       DASWNS010000481         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_69631, whole genome shotgun sequence.
ACCESSION   DASWNS010000481 DASWNS010000000
VERSION     DASWNS010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69631"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..3013)
                     /gene="infB"
                     /locus_tag="VGB76_16585"
     CDS             complement(80..3013)
                     /gene="infB"
                     /locus_tag="VGB76_16585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY0100565.1"
                     /translation="MTVQSKVRIYDLAKELKLESKRLIEEVRREGVDVSVPSNTVSKE
                     LAEKIRNKYFPKKETVAPRTIRVVKKAHAPATAEPAPGEAEDIHVSVAPPAAAISPEP
                     APPTAPTALSQNGADASTATVDQVATAKPIIGRLVKKPLAPATRVEQPEQTPQQQSAP
                     ATATTEFEAPEPEQSITGAPVSETPALDAVGPVQLGANEAQVAAPAVEQLGEAPLTPV
                     ARGASVLPPPPPVAVTTPPPSKQVKVLRLNASALNEGIKPGERVTAPPAPVVQPTPRE
                     RDRRGREAERGRNTQRATGTPGESANPQTVYTPPADARRPRSRAGGARGRGGPVGGAD
                     ARGGFKGRGGRFDRDFVQPPKALTLEERIAGSLNVGTGGGGAVGTLKTARLVEGSTVK
                     EFAEKLGIKPKDIVTLLLQRGVFATINQPLDNKVAVDLGQRFGYEVTFVPFEEMQVEE
                     EFEELIETGADDLEVTRAPVVTVMGHVDHGKTSLLDGIRATDVAAGEAGGITQHIGAY
                     SVQVPNPDNPAEMRRVVFLDTPGHEAFTMMRARGAKATDVVVLVVAADDGVMPQTIEA
                     IEHSRAAGVPIIVAINKIDRPDANPQRVRQELAGHGVQAVEWGGDIEMVEVSAKQRKN
                     LDTLLETILLTTDILNLRASPTRRASGVVLEAKLDRGRGSVATVLVQQGTLRVHDPFI
                     AGQVFGKVRAMFDDKGASITEAGPATPVEVLGLQGVPQAGESFQVVDDISRAQSISEH
                     RQMVTRQNTLLQTTKRGIEALGEKEVKELRVVLKADVQGSVEVLKSTLTKLSTEAVKV
                     FVVRSGVGAITESDVLLASATQAGASNSAVVIIGFNVRPEVRAAELAKLEHVDIRLHS
                     IIYKVEEEIRAAMIGMLEAIESERILGKAEVRQVFKVPRAGTVAGCMVIDGTIRRNAR
                     ARVIRDGVVAWEGGIDSLRRVKDDVSEVREGFECGISLENFNDIKVGDQIEAYIVEKV
                     AATEL"
BASE COUNT          482 a         1061 c         1061 g          691 t
ORIGIN      
        1 gccggaaaca agacaagttt ttaactttta gcttttgctt tttaacctgt cacccgtcac
       61 ttgtcactcg tcactgcttt tacagttcgg tggctgccac cttctcgacg atgtacgcct
      121 cgatctggtc gccgactttg atatcgttga agttttcgag cgagataccg cactcgaagc
      181 cttcgcggac ttccgatacg tcgtctttca cgcggcgcag gctgtcgatg ccgccttccc
      241 acgcgacgac gccgtcgcgg atgacacgcg cgcgcgcatt gcggcggatc gtgccgtcga
      301 tgaccatgca accggccacc gtgcccgcgc gcggcacttt gaagacctgc cgcacttcgg
      361 ccttgccgag gatgcgctcc gactcgatag cttcgagcat gccgatcatg gccgcgcgaa
      421 tctcttcttc caccttgtag atgatcgagt ggaggcggat gtctacgtgt tcgagtttcg
      481 ccagttccgc cgcgcgcact tcggggcgca cgttgaaacc gatgatgacg accgcagagt
      541 tagatgcgcc tgcctgcgtg gccgatgcga gcaacacgtc cgactcggtg atcgcgccga
      601 cgcccgaacg cacgacgaag actttcaccg cttcggtcga gagcttcgtc agcgtgctct
      661 tcaacacttc gaccgacccc tgcacgtcgg ccttcaggac gacgcgcagt tccttgactt
      721 ccttctcgcc gagcgcttcg ataccgcgct tcgtcgtttg cagaagcgtg ttctggcgcg
      781 tcaccatctg gcgatgctcc gagatgctct gcgcgcgcga gatgtcgtca accacctgga
      841 acgactcgcc cgcctgcggt acgccttgca gcccgagcac ctcgacgggc gtggccggtc
      901 cggcttccgt gatgctcgcg cccttgtcgt caaacatcgc gcgcaccttg ccgaagacct
      961 gccccgcgat gaacggatca tgcacccgga gcgtgccctg ctggacgagg acggtggcga
     1021 ccgaaccgcg accgcgatcg agtttcgctt cgagcacgac gccggaggcg cgtctggtcg
     1081 ggctggcgcg aaggttgaga atgtctgtgg tgagcaggat ggtttcgagc agcgtgtcga
     1141 ggttcttgcg ctgcttcgcg gagacttcga ccatctcgat gtcgccgccc cactcgacgg
     1201 cctgcacacc atgacccgcg agttcctgcc gcacgcgctg cgggttggcg tccggcctgt
     1261 caatcttgtt gatggcgacg atgatcggca cgcccgccgc gcgcgagtgc tcgatggctt
     1321 cgatggtctg cggcatcacg ccgtcgtctg ccgcaacgac gagcaccacc acgtcggtcg
     1381 ctttcgcgcc gcgcgcacgc atcatcgtga aggcttcgtg gccgggcgtg tctaagaaga
     1441 cgacgcgccg catctccgca gggttgtcgg ggttcggcac ctgcacgctg tacgcaccga
     1501 tgtgctgcgt gatgccgccc gcttcgcccg ccgccacgtc ggtcgcgcgg atgccgtcga
     1561 gcagagaggt cttgccgtga tcgacgtgtc ccatgaccgt gactacggga gcgcgcgtaa
     1621 cttccaggtc gtccgcgccc gtctcgatca actcttcgaa ctcttcttcg acctgcatct
     1681 cttcgaacgg gacgaaggtg acttcgtagc cgaagcgttg gccgagatca accgccactt
     1741 tgttgtcgag cggttggttg atggtggcga agacgccgcg ctgcaacagg agcgtcacga
     1801 tgtccttcgg cttgatgccg agcttttcgg caaactcttt aacggtcgag ccttcgacga
     1861 gacgcgcggt tttgagcgtc ccgaccgcgc ccccgccgcc cgtgccgacg ttgagcgatc
     1921 cggcaatgcg ttcttcgagc gtaagcgcct tcggcggttg cacgaagtcc cggtcgaagc
     1981 gtccgccacg ccctttgaat ccgccgcgtg cgtccgcgcc gcccacaggg ccgccgcgtc
     2041 cgcgtgcgcc gcccgcgcgt gaacgcggcc tgcgcgcatc tgcgggcggt gtgtagacgg
     2101 tctgcgggtt cgcgctctcg ccgggcgtgc ccgtggcgcg ctgcgtattg cgcccgcgtt
     2161 cggcctcgcg cccgcgacgg tcgcgctcgc gcggagtcgg ttgcacgacg ggagcgggcg
     2221 gtgcggtcac gcgctcgccg ggtttgattc cttcgttaag cgcggaggcg ttcaggcgca
     2281 gaactttgac ctgcttcgag ggcggcggtg tggtaacggc gacgggcggt ggcggcggca
     2341 agacgctcgc tccccttgcg acgggcgtga gcggcgcttc gccgagttgt tccacggcgg
     2401 gcgcggccac ttgcgcttcg ttcgcaccga gttgcacggg accaaccgca tcgagcgcgg
     2461 gcgtctcgga gacaggtgcg ccggtgatgg attgttcggg ctcgggagcc tcgaattcag
     2521 tggttgcggt cgcgggtgcg ctctgctgct gcggcgtctg ctccggttgc tccacgcggg
     2581 ttgcgggcgc aagcggtttc ttgacgagcc gcccgatgat gggcttcgcc gtggcaacct
     2641 gatcgaccgt tgcagtgctc gcgtccgcac cgttctggct caaagccgtg ggcgcggtcg
     2701 gcggtgccgg ttcgggtgag attgcagcgg cgggcggcgc gacggagacg tgtatgtctt
     2761 ccgcttcacc gggcgcgggt tcggcggtgg ccggagcgtg cgcttttttg acgacgcgaa
     2821 tggtgcgcgg cgcaaccgtc tctttcttgg ggaaatactt gttgcgaatc ttctccgcca
     2881 gctctttcga aaccgtgttg gagggcacgc tcacgtcgac gccctcgcgg cgcacctctt
     2941 cgataagacg cttcgattcg agcttgagtt ctttggcgag atcgtagata cgaactttgg
     3001 actgaacggt catgcacttc cttctcgacg ctttggtaaa acggcgtcgc gcgctttaaa
     3061 ggttgaaaga ctctcgcctc tttcgtgacc tctgacttaa cagcgcgcga tgctttaaaa
     3121 aaaagcgtcg ggacacgtgc cgccgagagg ccgtctaagg cgagttacta caagccgcgc
     3181 cgctcatccg agcgacgcgc ttggtgcggt tattctgcgc tgcccgaagc atcttgcttc
     3241 tcgtctgcgg cagcgtctcg ttcatcgttc gtctcgaccc ggtactcggg tgcgg
//
LOCUS       DASWNS010000482         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_407341, whole genome shotgun sequence.
ACCESSION   DASWNS010000482 DASWNS010000000
VERSION     DASWNS010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407341"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VGB76_16590"
     CDS             complement(<1..726)
                     /locus_tag="VGB76_16590"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY0100566.1"
                     /translation="MSAHARAELKTLDAPVPASSKSARSVTGLLQRKCACGGSAGMNG
                     TCGGCQRKTLNRSRSASQAASSFSHETTTAQAAAAPLIEEVLGSAGQPLDAATRSLME
                     TRLGHDFSHVRVHTGARAAQSAQAVGALAYTVGRQVVFDADQYQPSTGAGRRLLAHEL
                     THVVQQGGGSRFQGKLEIGSPSGADEREADEVAANFEQGAEAGKPAPRPKRSGAPPVL
                     RRKTGGAGSWFTGLFSSLVLGTVG"
     gene            complement(723..983)
                     /locus_tag="VGB76_16595"
     CDS             complement(723..983)
                     /locus_tag="VGB76_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100567.1"
                     /translation="MRASEIELHIEELVLDGFAHADRYRIGDTFERELSRLLSEQGVP
                     SALVADGLQTHLDAGACRIAAGSTAEHIGAQLAQAVYGGLKG"
     gene            complement(980..3199)
                     /locus_tag="VGB76_16600"
     CDS             complement(980..3199)
                     /locus_tag="VGB76_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0100568.1"
                     /translation="MSASAGNNWHEANQRYLMARLAFVRDALKRHAARHSIAGGAGGE
                     GWTRAQASETNRAPDEATWKVASPADAPQEVVLREDASQEVAASEGAEDETERRLREA
                     AEALPAPAALDSLCEAFGLSPFERDVLLMCAGMELDATFAPLCAAAQGNANRVHPTFG
                     LALAALPEAHWNAITPDAALRRWRLVEVWQNEGTLAQSGLRIDERVLHYLTGISYLDE
                     RLRGLVEPVPLEHDLPPSQFALVEGITHFWRQSRGGSLLPFVFLSGNEDAAGKRLVAA
                     HSCATLGLRLHVLRAADIPLPVAEREALSVLWQREAVLQASALLIEDDDAEKTPPHAV
                     LSFIESMHGMLFVAGSEMLRMRQRQSLRLNVDKPDAAEQRVLWREALGHVSDELNVQL
                     DNVISQFSLTGDKIRAASAQALLELARAEGAERAASSGTGTAGTGAAGAEEVSAQEQG
                     AERTPAEILWDTCRIQARTRLDDLAQRIEPIARWSDLVLPEHQTRSLLEIAAHVRQRS
                     RVYETWGFAAAGARGLGISALFAGASGTGKTMAAEVIARELRLDLYKIDLSQVVSKYI
                     GETEKNLRRVFDAAEEGGAVLLFDEADALFGKRSDVKDSHDRYANIEISYLLQRMESY
                     RGLAILTTNMKTSLDTAFMRRLRFVVQFPFPDAAQRGEIWRRVFPPQTPTENLEIRKL
                     ARLNVAGGNIRNIALHAAFHAADTGEAVTMSHILRAARSEYDKLEKTLTESESGGWL"
BASE COUNT          535 a         1135 c         1084 g          617 t
ORIGIN      
        1 gccgacggtg ccgagaacga gactggagaa cagacccgtg aaccaacttc ccgcgccgcc
       61 cgtcttgcgc cgcagaacgg gcggcgcgcc tgaacgcttc gggcgcggcg cgggctttcc
      121 ggcctccgct ccctgctcga agtttgccgc cacctcgtcg gcctcgcgct cgtccgcgcc
      181 ggagggcgaa ccgatctcaa gtttgccctg aaagcggctg cctcctccct gctgtaccac
      241 gtgcgtcaac tcgtgcgcga gcagacgcct gcccgcaccc gtcgaaggct ggtactggtc
      301 tgcgtcgaag accacctgcc tgccgaccgt gtaggccagc gcgcccacgg cctgcgcaga
      361 ctgcgccgca cgcgcgcccg tatgcacgcg gacgtggctg aagtcgtggc cgagacgcgt
      421 ctccatgagc gagcgcgtgg ccgcatcgag cggctgaccc gcagagccga gcacctcttc
      481 gatgagcggc gcggctgcgg cctgcgcagt agtagtctcg tgcgagaatg aagatgcggc
      541 ctgactcgca gaacgcgagc ggttgagagt tttgcgttga cagccgccgc acgttccgtt
      601 catgcccgcc gagccgccgc aggcgcactt gcgttgcaag agtccggtca cgctccgcgc
      661 gctcttcgag gaggcgggaa cgggcgcgtc caaagttttc aactccgctc tggcgtgcgc
      721 gctcatccct tcaacccccc gtagactgcc tgcgccaact gtgcgccgat gtgctccgcc
      781 gtagagcctg cggcgatccg gcatgcgccc gcgtcgaggt gcgtctgtag gccgtcggcg
      841 acgagcgcag agggcacgcc ctgctcggac aggagacgcg acaactcgcg ctcgaacgtg
      901 tcgccgatgc ggtagcggtc ggcgtgtgcg aagccgtcga gcacgagttc ctcgatgtgc
      961 agttctatct cagacgctct cacagccagc ctccgctttc gctctcagtc agagtcttct
     1021 ccaacttgtc gtactcgctg cgggcggcgc gcaggatgtg cgacatcgtg acggcctcgc
     1081 ccgtgtctgc ggcgtggaag gccgcatgga gcgcgatgtt gcgaatgttg ccgcccgcca
     1141 cgttgagccg tgcgagtttc cgtatctcaa ggttctcggt gggcgtttgc ggaggaaaga
     1201 cgcgccgcca gatttcgccg cgctgcgctg cgtcgggaaa cggaaactgc acgacgaaac
     1261 gcagccgccg catgaaggcc gtgtcgagcg aggtcttcat gttggtcgtg agaatcgcca
     1321 agcccctgta ggattccatg cgttgcagga gataactgat ctcgatgttg gcgtagcggt
     1381 cgtggctgtc tttgacatcg ctgcgtttgc cgaagagcgc atcggcctca tcgaagagga
     1441 gcaccgcgcc gccctcttcc gccgcatcga agacgcgccg gaggttcttc tccgtctcgc
     1501 cgatatactt gctgaccacc tgcgagaggt cgatcttgta gaggtcgagc cgcaactcgc
     1561 gcgcgataac ttccgccgcc atcgtcttgc ctgtcccgct cgcccccgcg aacaacgcgc
     1621 tgatgccgag gccgcgcgca cctgcggcgg cgaagcccca cgtttcgtag acgcggctgc
     1681 gctggcggac gtgtgccgcg atttcgagca ggctgcgcgt ctggtgctcc ggcaaaacga
     1741 ggtcagacca gcgtgcgatt ggctcgatgc gttgcgccag atcatcgaga cgtgtgcgcg
     1801 cctgaatccg gcacgtatcc cagagaattt ctgcgggcgt cctctccgcg ccttgctcct
     1861 gcgctgatac ctcttctgcg cccgccgctc ctgtgcccgc cgtccctgtg cccgacgagg
     1921 ctgcgcgctc tgctccttcc gcgcgtgcca gttcaaggag agcctgcgca ctggccgcac
     1981 gaatcttgtc gccggtcagg ctgaactgcg aaatgacgtt gtcgagttgg acgttcaact
     2041 cgtcggaaac atggccgagc gcttcccgcc agaggactct ctgttcggct gcgtcaggct
     2101 tgtccacgtt caggcggagt gattggcgtt ggcgcatgcg cagcatctcg ctccccgcga
     2161 cgaagagcat tccgtgcatg ctctcgatga acgacaggac ggcgtgcggc ggtgtcttct
     2221 ccgcatcgtc atcttcgatg agcagcgcgc tggcctgcaa gacggcctcg cgttgccaga
     2281 gcacggagag cgcctctcgc tccgcgacgg ggaggggaat gtctgcggcg cgcagcacgt
     2341 gcaggcgcag tccgagcgtc gcgcagctat gagccgccac cagacgtttc cccgcggcgt
     2401 cttcgttgcc ggagagaaag acgaacggca ggagcgagcc gcccctcgat tgtctccaga
     2461 aatgcgtgat gccttcgacc agcgcgaatt gagacggcgg cagatcgtgc tcaagcggca
     2521 cgggttcgac aagcccgcgc aggcgttcat ccagatagga gattccggtc aggtagtgca
     2581 gcactcgctc gtcaatgcgc aacccgctct gcgccagcgt cccttcgttc tgccagactt
     2641 cgaccagccg ccaacgacgc agtgccgcgt ccggcgtgat agcgttccag tgagcttcgg
     2701 gcagagcggc gagcgcgagg ccgaaggtcg ggtggacgcg gttggcgttg ccttgagccg
     2761 ccgcgcagag cggggcgaaa gtggcgtcga gttccatccc ggcgcacatc agcagcacgt
     2821 cgcgttcgaa gggagacaaa ccgaaagctt cgcacaggct gtcgagggct gccggtgcgg
     2881 gtagtgcttc agccgcctct cgcagacgcc gctccgtctc atcctccgcc ccttcggacg
     2941 cggcaacttc ctgcgacgcg tcttcacgca gcacaacctc ctgcggcgca tcagcaggcg
     3001 aggcgacttt ccacgtcgct tcgtccggcg cgcggttcgt ctcgctggcc tgcgcgcgcg
     3061 tccatccttc gccgcccgct ccgcccgcta tggagtggcg cgcggcgtga cgcttgagtg
     3121 cgtcgcgcac gaaggcgagc cgcgccatca ggtaacgctg gttggcctcg tgccagttgt
     3181 tgcccgccga cgcgctcata gaatctctac ctgcggcgtg ctgaacctct tcgtcaaagg
     3241 gtctgtcttg agcacgctct cggctccgtc cacctgcaac cgcacgagat acttcccctt
     3301 tctcacgttc acaaacggaa tcgagacttc ggtgacggtt tcaggcggaa cgagcggcgg
     3361 cggaaaaatc t
//
LOCUS       DASWNS010000483         3093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_147931, whole genome shotgun sequence.
ACCESSION   DASWNS010000483 DASWNS010000000
VERSION     DASWNS010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147931"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..>3093
                     /locus_tag="VGB76_16605"
     CDS             43..>3093
                     /locus_tag="VGB76_16605"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0100569.1"
                     /translation="MLKNPLFGLNRMLSLCASLALMASFAFTQSQATSGNIEGRVLDP
                     NGAAVPGVSITATNQQTGFEKTAISDEEGHYRLIMLPPGTYKLVTASAQGFQPVTLEN
                     AVVTVGGQTNLDINLAVGHNDLSIDVSAEAPVVETTRTSVATTVNERDIENLPVSGRN
                     YLDFATLTPGVVRDPSRQGDLSVGGQKGTLNSLQVDGADNNNTFFGQAFGRTGVRPPY
                     QFSEESVKEFQVNQNGFSAEFGRAGGAVINVVTKSGTNSFHGGVFEYFRDESLNANAP
                     NIKGLQGADFDLGRRLDRNKRPPQQINQFGFRLGGPIVKNRAFFFGTYDGQRQDLPNI
                     VEPSNLGAQSAAIQDLLRPRLAPYQISRNQDVYLLKTDIAINGSNQLSLRFNRQNFTG
                     VNNEFTGALAAEEHSGDSLARTTTFSGTLASTITSSIVNELRFQFARDAEPGTANSDL
                     PETQIQTGNGFLLLGRNNFSPRETTIKRAQFINNLSYVRGQHNYKVGVDLNFDRILNF
                     FPGFFSGQYTFSPITVDGVTLNGYQAFQRGLPSGFQQRFALPGTSGATSNPNSTDYAL
                     FLQDDWRATPKLTLNLGLRYDYQKLAAPPLRNPDPALLAAGFDTSRQPDDGNNLAPRV
                     GFAYDVRGDGKSVVRGGYGIFYGRTTAIMLGTAHTGNGIQTTGVNFANNAAIVAAGLT
                     YPNIINTAPTAATGTPDIFLFSEDYAQPYVQQARLGFEHELMQNMSFSVTYLFFRGVH
                     LSRTRDINLFAPELVTATGAGQSFTVERFPAAGANSNARFTATAPARPFTRFGRINLF
                     ESTANSRYDGLAFQLQRRFSRGFQFLASYTYSKAKDDKPDQTAVVPGGGDDAKIVQNQ
                     LNIRDDYAAADSDLRHRFVFSPVYEFGKFTRSDNSVLRALLSDYTLSSIVQLQSGFAF
                     SQTVGADLNRDGNARNDRVPGTRRNEFYTPATYQFDARLTRSIFFGESMRLRLILEGF
                     NIFNRANVATVNTNLFAGRTAAINSSGAQVLTLSSPAASVGYGLP"
BASE COUNT          607 a         1164 c          800 g          522 t
ORIGIN      
        1 tcaatctgtc acccgcgatc cgtttccagt tgaaaggaga tcatgctcaa aaatccactt
       61 ttcggtctga accggatgct ctcgctgtgt gcgtcgctcg cgctcatggc gagtttcgct
      121 ttcactcaat cgcaggccac ctccggcaac atcgaagggc gcgtgctcga cccgaacgga
      181 gccgccgtgc ccggcgtcag catcacggcg accaaccagc agaccggatt cgagaagacc
      241 gccatctccg acgaagaagg ccactatcgc ctgatcatgc tcccgcccgg cacgtacaaa
      301 ctcgtcaccg cgagcgcgca gggctttcag ccggtaacgt tggagaacgc cgtcgtcacg
      361 gtcggcgggc agacgaacct cgacatcaat ctcgccgtcg gccataacga cctgtccatc
      421 gacgtgtccg ccgaagcgcc cgtcgtcgaa acgacgcgca cgtccgtcgc caccaccgtc
      481 aacgaacgcg acatcgaaaa cctgccggtg agcgggcgca actatctgga cttcgccacg
      541 ctcacgcccg gcgtcgtgcg cgacccctcg cggcagggcg atctctccgt cggcggccag
      601 aagggaactc tgaacagctt gcaggttgac ggcgcagaca acaacaacac cttcttcggt
      661 caggcgttcg ggcgcacggg cgtgcgtccg ccttatcaat tctcggaaga atcggtcaaa
      721 gagtttcagg ttaaccagaa cggtttctct gcggagttcg ggcgcgcggg cggcgcggtc
      781 atcaacgtcg tcacgaaatc cggcacgaac agttttcacg gcggcgtctt cgaatatttc
      841 cgcgacgaat cgctgaacgc caacgcgccg aacatcaaag ggctacaggg cgcggacttc
      901 gacctcggac gccgcctcga ccgcaacaag cgaccgccgc aacagatcaa ccagttcggc
      961 tttcgtctcg gcggccccat cgtcaagaat cgcgccttct tcttcggcac ctacgacggc
     1021 cagcggcaag acctcccgaa catcgtcgaa ccctcgaacc tcggcgcgca gtcggcggcc
     1081 atacaggatt tgctgcgccc gcgcctcgcc ccttaccaga tcagccgcaa tcaggacgtg
     1141 tacctgctca agaccgacat cgccatcaac gggagcaacc aactctccct gcgcttcaac
     1201 cggcagaact tcacgggcgt caacaacgag ttcaccgggg cgctcgccgc cgaagaacat
     1261 tccggcgaca gtctcgcgcg caccaccact ttctccggca cgctcgcctc caccatcacc
     1321 tcctccatcg tcaacgaact tcgtttccag tttgcgcgcg acgccgaacc gggcacagcc
     1381 aacagcgatc tcccggagac gcagattcaa accggcaacg gcttcctcct gctcggtcgc
     1441 aacaacttca gcccgcgcga gacgaccatc aaacgcgcgc agttcatcaa caacctctct
     1501 tacgtgcgcg gccagcacaa ctacaaagtc ggcgtggatt tgaacttcga ccgcatcctg
     1561 aatttcttcc ccggcttctt ctccggccag tacaccttct ccccgatcac ggttgacggc
     1621 gtgaccttaa acggctatca ggcgttccag cgcggcctcc cctccggctt ccagcaacgc
     1681 ttcgcgctgc ccggcacatc cggcgcgacc agcaatccca acagcaccga ttacgccctc
     1741 ttcctacagg acgactggcg cgccacgccc aaactcacgc tcaatctcgg cctgcgctac
     1801 gactatcaga aactcgctgc gccgccgctc cgtaatccag accccgcgct gctcgccgcc
     1861 ggattcgaca cgagccgcca gcccgacgac ggcaacaacc tcgcgccccg cgtcgggttc
     1921 gcctacgacg tgcgcggcga cggcaagtcg gtcgtgcgcg gcggctacgg catcttctac
     1981 gggcgcacca ccgccatcat gctcggcacg gcgcacaccg gcaacggcat ccagaccacc
     2041 ggcgtcaact tcgccaacaa cgccgccatc gtcgccgcag gtctgaccta cccgaacatc
     2101 atcaacaccg cgccgaccgc cgccaccggc acgcccgaca tcttcctctt ctccgaagac
     2161 tacgcgcagc cctacgtgca acaggcgcgt ctcggcttcg agcatgagtt gatgcagaac
     2221 atgagtttct ccgtcaccta tctcttcttc cgaggcgtcc acctctcgcg cacgcgcgac
     2281 atcaaccttt tcgcccccga actcgtgacg gcgacgggcg cggggcagag cttcaccgtc
     2341 gaacgcttcc ccgccgccgg ggcaaactca aacgcgcgct tcacggccac cgccccggct
     2401 cgccccttca cacgtttcgg tcgcatcaac ctcttcgaga gcaccgccaa ttcgcgctac
     2461 gacggcctcg ccttccagtt gcagcgtcgc ttctcgcgcg gcttccagtt cctcgcctcc
     2521 tacacctatt cgaaggcgaa ggacgacaag cccgaccaga cggcggttgt gcccggcggc
     2581 ggcgacgacg ccaagatcgt gcagaaccaa ctcaacatcc gcgacgacta cgcggcagcc
     2641 gattcagacc tccgccatcg cttcgtcttt agtcccgtct acgaattcgg caagttcacg
     2701 cgcagcgaca actccgtgct gcgcgcgctt ctcagcgact acactctgtc gagcatcgtg
     2761 caactacaat ccggcttcgc cttctcgcag accgtcggcg cggatttgaa ccgcgacggc
     2821 aacgcacgca acgaccgcgt ccccggcaca cgtcgcaatg agttctacac gcccgcgacc
     2881 taccagttcg acgcgcggct cacgcgttcg atcttcttcg gcgagagcat gcgcctgcgc
     2941 ctcatcctcg aaggcttcaa catcttcaac cgcgccaacg tggcgacggt caacaccaat
     3001 ctcttcgccg ggcgcaccgc cgcgatcaac agcagcggcg cgcaggtctt gacgctctcg
     3061 tcgcccgccg cgtcggtggg ctacggactc ccg
//
LOCUS       DASWNS010000484        10952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_381107, whole genome shotgun sequence.
ACCESSION   DASWNS010000484 DASWNS010000000
VERSION     DASWNS010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381107"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..860)
                     /locus_tag="VGB76_16610"
     CDS             complement(267..860)
                     /locus_tag="VGB76_16610"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEY0100570.1"
                     /translation="MRRPRRKANVRDINETDAKPKRLIDPEKSRTRTLQRAVKLLAAK
                     PRSTAELRERLLEKEWTTPEAADYAIAKLSEYGYLNDEQFALGYASARVRQKPVGRGR
                     LARDLQMKKIDRELAEQTLEKVFDETSESELIDRAIERRTRLRGRPTTRQETKSLFDH
                     LLRRGFSYDLVINKVRALASGKLEEDEEAGSDANDSL"
     gene            1048..2148
                     /locus_tag="VGB76_16615"
     CDS             1048..2148
                     /locus_tag="VGB76_16615"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY0100571.1"
                     /translation="MDGVLIIDKPVGWTSHDVVARVRRILRERRVGHTGTLDPFATGV
                     LVVLVGRATRLAQFLAGAEKEYEARVRFGYATETGDLTGARLDELPQTEDSKEDARQG
                     EATQEDVSKGEATKREATKGEATKAGGLPCAEWSVEEIEAAMASLRGEIEQVPPMYSA
                     KKVQGKKLYELARRGVRIERAAVRVTIHELEALKATDGGAWLRRENSDGTCELDLRVV
                     CSAGTYIRVLAEDLGACLGAGAHLAALRRTRAGAFGIEKALTLESLAERVEAGALVEL
                     LITPDAALPQMPFVHLTGEALRRARHGAAVRVGADEPGLPSAARASLKLRDGELVKMR
                     DGEAGKLIAVGVYDAGRASLQPRVMLDASEEK"
     gene            2508..4448
                     /locus_tag="VGB76_16620"
     CDS             2508..4448
                     /locus_tag="VGB76_16620"
                     /inference="COORDINATES: protein motif:HMM:NF019902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100572.1"
                     /translation="MHEEQDGKYKFNISQRLEDLRREHESLQWEGSFRDYFELVSQNP
                     RLAQLSHARVNDMIHAAGIEKLNEGTRDEVARYRFFADELYGIEEPLARIVEYFKSAA
                     QRLEVRKRILLLMGPVGGGKSTIVTMLKRGLEEYTRTEAGAAYAIKDCPMHEEPLHLI
                     PHELRAEIEKHYGIYIEGDLCPQCRYALEHTYGGRHEDVRVQRILFSEKERVGIGTFA
                     PSDPKSQDITELTGSIDLSTIGEVGVESDPRAYRFDGELNIANRGLMEFIEMLKVDEK
                     FLYSLLTLSQEQNIKTGRFAMLYADEVILSHTNENEYISFAGNRKSEALQDRIILVRV
                     PYNLRVSQEERIYGKLLNQSEVLRNVHIAPNTLRVAAMFAVMTRLEEPKRANVDIVKK
                     MKLYDGEDVEGYKSKDVRELRDETVREGMDGISPRYIINRLSGALVRDGVTCINPIDA
                     LRAIKDGFEQHTGINPEQRERYLNLISASRKEYDELAKIEVQRAFVYSFEEMARTICN
                     NYLDNVEAYCNKERMKDPITEEEMEPDEQLMRSIEEQIGVSDNAKNTFRQEILIRISS
                     FSRKGKQFEYTSHERLKEAIEKKIFADLKDVVKITTSSRTPDPDQLRRINEVVDRLVK
                     DGGYCPVCANELLTYVGTLLSR"
     gene            4558..5541
                     /locus_tag="VGB76_16625"
     CDS             4558..5541
                     /locus_tag="VGB76_16625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0100573.1"
                     /translation="MKTRYWIGGTTALVGGALALKLLTRPQDVEWDEHRERLLHAEKS
                     RFVEIEGVRVHYQEAGASDAPPVLLIHGFCASNFVWSETLVPLAEAGFRVIAPDLIGF
                     GFSGKPEGGAYTIETQARMICGLMDALGIERAVLVGSSYGGAVAAVCALDHASRVERL
                     VLVGAVSNDDVPRRVWLRLAASPVVGEVISPVLLDARRLVKRRLRRTYAAQNNLLFDE
                     ERITAHQRPLLAANTHRAILRTLRSWSAERIELEAARITQPTLLVWGAHDSEVPLSSG
                     KRLHELIEGSRLLVFPNCGHLPQEERPREFASLVAEFCRETRGTDEASGGA"
     gene            5559..6719
                     /gene="yhbH"
                     /locus_tag="VGB76_16630"
     CDS             5559..6719
                     /gene="yhbH"
                     /locus_tag="VGB76_16630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HEY0100574.1"
                     /translation="MSVQRNDWSLQRKGIIDQERHKERVKDAIRKNLGSIVSNEAIIL
                     SDGKRSVKVPIRSLDEYKFRFDHRKRKHVGQGDGKTRVGDVLAREQGQGQGSGSGPAG
                     QETGADYYEAEVNIDELAALIFEDLHLPFLEEKAKKAVPAKTTRFNEIRRTGVMANLD
                     KRRLILENIKRNAREAKGAKFGNVKKEDLRFKSWEEEHRYESNAVVLALMDVSGSMGE
                     FKKYIARSFFFWMVRFLRTKYDNVEIVFISHHTEAKEVSEEQFFTQGESGGTVVSSAY
                     ALALDIIAARYNPRDWNVYPFHFSDGDNYYSDNEEAVRLADELIQTCNLFGYGEIGEE
                     GTSGYRRSSGALLSIFKDRLQRKERFVGVRIDEREDVYPALKQFFGQRGVEA"
     gene            7206..10457
                     /locus_tag="VGB76_16635"
     CDS             7206..10457
                     /locus_tag="VGB76_16635"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0100575.1"
                     /translation="MNNRFKKWLATFLSLSMIMLSLSVPAGAQEPTGAIEGTVTDPQK
                     AIVLNATVTARNTDTNLSRTVTTGDDGNFSIRQLPPGNYEVTITSANFKKSVYKDVTV
                     AVGQNASLDAELQVGGATEEVTIVGTSEAQIDRVDHNVSGVVGTVQIQNLPLNGRNFL
                     DLAQLQPGVEKVEGGSFDPTKANYTGISIAGQAGRSTQIAVDGGSVVDNVVGTTVQNF
                     SQEIVQEFQLGISNYDLSTGASSTGSVNVISKSGSNDFHGNAYIYARDDKWSAFPSLG
                     RLDAANNIPATAQATRIPFDRQQYGFTLGGPINRDRLFFFFNYEGNNQDGSALHNPTE
                     APSFAGFTANPFDEKLLTGKIDWIVSNKTNISTRYSFNDNFQDVPFAPGSGIVPRASA
                     SRIFESNNQLVSNRSHNVVIGTTHAFSPSVTNNFVVNISDFSNEIDPAVEGVPEIRIL
                     GSQLFRTGTNAIAPQSTYQERHQLRDDLTMIRGNHTFRIGGNYERTSINGTFAFAKPA
                     RIRLFNPADLEHPLPPYQTEAEFLNAPVRDISMGIGSDVLPFNSDRSDTLNHRFQFYG
                     NDTWKLANRFTMNFGLSYRIDSNLWNHDQPRPAIVSSLFGKGTAASPRDKNNIAPRVG
                     FAWDVAGNGKTVVRGGIGIYYDNTIDNLRLFERADLGRPGAELFLVGVDLRSPLFPGG
                     DGRFSEGQLTLAQALALVGPARADIESRQFNCPAGQTSLECAEAISGPLFSTEFQVPY
                     SIQYAVGVQRELPWKMLFQADFNYRKGLHEVIVYDINKIDDDITGPRSAFPNTVPYAD
                     SSGFSTYMAGLFRLDRRFSNGFQMTASYSLSRFKAFGGDALGLGATVTDLNNFRPEFG
                     PAGLDRTHRFVVSAIYELPFFRTSSNAFKRNVLGGWQVSMISTAFSGIPQSVFLPHFA
                     DLSRTGTFQTYLPGTRNGSIGRDIRNVADLNSLITAYNASIPTLGVDCGLENSPTGRC
                     DSTHPSYFDPLTRLALLPADTQLGGDSIISQDVRLTKTFRFGERSSLDLIGEVFNLFN
                     VANLTYPAAIALSDEGTSASDVRNVTHGASARTTSVFGTGGPRAFQFAAKFRF"
     gene            10485..>10952
                     /locus_tag="VGB76_16640"
     CDS             10485..>10952
                     /locus_tag="VGB76_16640"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0100576.1"
                     /translation="MEPETWLHHFFIVASEVAAETGDPATRSTFELSSLERIPAMRQR
                     SALLALTFTLCCYFFFCAAATSAHAPQGHTIRGKVRSSAGFNVPRVTVNLESGGGALI
                     DQTVTNNEGDFFFGGLDNTSYQITVSAPDYNPASERVEFVRNVGASEPGETRTV"
BASE COUNT         2203 a         3420 c         3312 g         2017 t
ORIGIN      
        1 cgttggcggg caaaagcggg ctggaacgca cgcgcgtatg ggcgttctgc tcaaagtagt
       61 ttaagaatat ttcgcgtatt tcgtttccgg tcttcttcat aaaatataat caactgacag
      121 cgttcaaccg ccgtttttcc atactcaagt ttgacttatg agtctatcac gcgaggcgcg
      181 ggcgggtgaa acgtgagggg cggcagagcg tctggccgga gtgtgaaagg agtgcgcgcg
      241 tgacgcgacg gcgaatcgcg gcgtcgctat agggaatcgt tcgcgtcgct gccagcctct
      301 tcgtcctctt caagtttgcc ggaggcgagc gcgcggactt tgttgatgac gagatcgtag
      361 ctgaagccgc gacgcagtag atgatcgaag agactcttgg tttcctgccg ggtggtgggg
      421 cgaccgcgca ggcgcgtgcg gcgttcgatg gcgcggtcta tgagttcgga ttcggaggtt
      481 tcgtcgaaaa ctttttcgag ggtctgttcg gcgagttcgc ggtcgatctt cttcatttgc
      541 agatcgcgtg cgagccgccc gcgcccgacg ggcttctgcc ggacgcgcgc cgaagcgtag
      601 ccgagcgcga actgttcgtc gttcaggtag ccatactcgc tgagtttagc gatggcgtaa
      661 tcggcggcct ccggcgtcgt ccattctttt tcgaggaggc gttcgcggag ttcggcggtg
      721 gagcgcggct tggcggcgag cagtttaacg gcgcgttgca gtgtgcgcgt gcgcgacttc
      781 tccgggtcga tcagacgttt cggcttcgcg tccgtctcgt tgatgtcccg cacgttggct
      841 tttcgtctcg gcctgcgcat ggatggatta aaacataggg gaaagggaaa agggtaaggg
      901 tgaagggaag cggcgcaggg cgaggggtag aggggcaggg ggaagggaga gaaaactgct
      961 ggccgccgtg acttgagcgt tgcttacgcg aagcgagaaa tgcttccgcg cctttcgcct
     1021 ttcccctttt ccccttaccc tgtcgtcatg gacggagtgc tgatcataga caagcccgtg
     1081 gggtggactt cgcatgacgt ggtggcgcgc gtgcgtcgca tcttgcgtga gcgacgcgtg
     1141 gggcacacgg ggacgctcga tccttttgcg acgggtgtgc tcgtcgtgct cgtcgggcgc
     1201 gcaacgcggc tcgcgcagtt tctcgcgggc gcggagaaag agtacgaggc gcgcgtgcgt
     1261 ttcggctacg cgacggagac gggcgacttg acgggcgcac gcctcgacga gttgccgcag
     1321 acggaagact cgaaggaaga cgcgcggcag ggagaggcga cgcaggaaga cgtgtcgaag
     1381 ggagaagcga cgaagcgaga ggcgacgaag ggagaagcga cgaaagcagg agggctgccg
     1441 tgcgcggagt ggagcgtgga agagatcgaa gcggcgatgg cgagtttgcg cggcgagatt
     1501 gagcaggtgc cgccgatgta ctcggcgaag aaggtgcagg ggaaaaagtt gtacgagttg
     1561 gcacggcgcg gcgtgcggat agagcgcgca gccgtgcgcg tgacgattca cgaattggaa
     1621 gcgttgaagg cgacggacgg cggcgcgtgg ctccggcgcg aaaactcaga cgggacgtgc
     1681 gaactcgacc tgcgtgtcgt ctgttcggcg gggacttata tccgcgtgct ggccgaagac
     1741 ctcggcgcgt gtctcggcgc gggcgcgcac ctcgccgcgc ttcgtcgcac gcgtgcgggc
     1801 gcgttcggca tcgagaaagc gttaacgctg gagagtctgg cggaaagggt cgaggcgggc
     1861 gcgctcgtcg aactgctcat cacgccggat gcggcgctcc cgcagatgcc tttcgtgcat
     1921 ctaacgggcg aagcgttacg ccgtgcgcgt cacggcgcgg cggtgcgagt gggggcagac
     1981 gaacccggcc tgccgtccgc cgcccgcgca tctttgaagc tgcgcgacgg cgaactcgtg
     2041 aagatgcgcg acggcgaagc gggaaagctg atcgccgtcg gcgtgtacga tgcggggcgc
     2101 gcgagtttgc agccgcgcgt gatgctggat gcgtccgagg aaaaataagc gttgattttc
     2161 tgtcccgcgt gctgtgttat aatcgcggca agcggtgaag gtcagatgtc gcgaaagcga
     2221 aacgcccgcg cttcctcccg tccgccgctc ccgtttgaca actttgacaa caacgacccg
     2281 gcaggatttc cgaaagactc gacggcgagg ggcttggaac gcatccgcgc acgtcccgcc
     2341 caacgcatcg aggcttgtcc taaatactta tcacggctct ccggttcaag cgtcacatcg
     2401 agacgaaact caaaccggag cagcccgtga gtcgtcatac aaccccagga agggcgtgac
     2461 ttccccacgc cgtccacgcg cgaagccgtt ttagaggtat caaggctatg cacgaagagc
     2521 aggatggcaa atacaaattc aacatcagcc aacggctcga agatttacgc cgcgaacacg
     2581 aatccctcca atgggaaggc tctttccgcg actacttcga gttagtctca caaaacccgc
     2641 gtctcgccca actctcccac gcgcgcgtca acgacatgat tcacgccgcc gggatcgaga
     2701 agctcaacga aggcacgcgc gatgaggtgg cgcgctacag gttcttcgca gacgaactct
     2761 acggcatcga agaaccgctc gcgcgcatcg tcgaatattt caagtcggcg gcgcagcgtc
     2821 tggaagtccg caagcgcatc ctcttgttga tggggcctgt cggcggcggc aagtcaacca
     2881 tcgtcacgat gctcaagcgc ggcctcgaag agtacacgcg cacggaggcg ggcgcggcct
     2941 acgcgatcaa agattgcccg atgcacgaag agccgctgca tctcatcccg cacgaactcc
     3001 gcgccgagat cgagaaacat tacggcatct acatcgaagg cgacctctgc ccgcagtgcc
     3061 gttacgccct cgaacacact tacggcggca ggcatgaaga cgtgcgcgtg cagcgcatcc
     3121 tcttctccga aaaagagcgc gtcggcatcg gcacgttcgc tccctccgat ccgaaatcgc
     3181 aggacatcac ggaactcacc ggctccattg atctctcgac catcggcgaa gtcggagtcg
     3241 agtcagaccc gcgcgcctat cgcttcgacg gcgaactcaa tatcgccaat cgcggcctga
     3301 tggagttcat cgagatgctc aaagttgacg agaagtttct ctactcgctc ctcacgctct
     3361 cgcaggaaca gaacatcaag accggacgct tcgccatgct ctacgcagac gaagtgatcc
     3421 tctcgcacac gaacgaaaac gagtacatct ctttcgcggg caatcgtaaa tcggaagcct
     3481 tgcaggatcg catcatcctc gtgcgcgtgc cttacaacct gcgcgtctcg caagaggaac
     3541 gcatctacgg gaaacttctg aaccagtcgg aagtcctgcg caacgtccac atcgcgccga
     3601 acactctgcg cgtcgcggcg atgttcgccg tcatgacgcg cctcgaagag ccgaagcgcg
     3661 ccaacgtgga catcgtcaag aagatgaaac tctacgacgg cgaggatgtc gaaggttaca
     3721 agtcgaagga cgtgcgcgaa ctgcgcgacg agactgtgcg cgaaggcatg gacggcattt
     3781 cgccgcgcta catcatcaac cgcctgtcgg gcgcgctcgt gcgcgacggc gtcacctgca
     3841 tcaacccgat tgacgcgctg cgcgccatca aggacggctt cgagcagcac accggcatca
     3901 accccgaaca gcgcgaacgc tacctcaatt tgatctccgc ctcgcgcaag gaatacgacg
     3961 aactggcgaa gatcgaagtg cagcgcgcct tcgtctattc gttcgaggag atggcacgca
     4021 ccatctgcaa caactatctc gacaacgtcg aagcctactg caacaaggaa cgcatgaaag
     4081 acccgatcac cgaagaggag atggagccgg acgaacaact catgcgctcc atcgaagaac
     4141 agatcggcgt ctcggacaac gcgaagaaca ctttccggca ggagattctg attcgcatct
     4201 cctctttcag ccgcaaaggc aagcagttcg aatacacctc gcacgagcgt ctcaaagagg
     4261 ccatcgagaa gaagattttc gcagacctca aggacgtggt gaagatcact acctcttcgc
     4321 gcacgcccga tccggatcaa ctgcggcgca tcaacgaagt ggtggatcgt ctcgtcaaag
     4381 acggcggcta ctgccccgtc tgcgccaatg aactcttaac ctacgtcggc acgctgctct
     4441 cccgctgagc ggggcggcgc gtgttcgtca acagagcttg cgcacgtctg attttgagtt
     4501 ccatgcccgc gcctttcgtc tctgaaaagc gcgggcgttt ggcttaaatg taaacgcatg
     4561 aaaacacgtt actggatcgg cgggacgacg gctctggtgg gcggcgcgct cgccttgaaa
     4621 ttgttgacgc gcccgcagga cgtggagtgg gacgagcatc gtgagcgcct gcttcacgcg
     4681 gagaaatcgc gcttcgtcga gatcgaaggc gtgcgcgtgc attatcagga agcgggcgcg
     4741 agcgatgcgc cgcccgtgct gttgattcac ggtttctgcg cgtcgaactt cgtctggagc
     4801 gagacgctcg tgccgctggc ggaagcaggc tttcgggtga tcgcgcccga tctcatcggc
     4861 ttcggatttt cgggaaagcc ggagggcggc gcgtacacga ttgagacgca ggcgcgcatg
     4921 atctgcgggt tgatggacgc gctcggcatc gagcgtgcgg tgctggtggg cagttcctac
     4981 ggcggcgcgg tggctgccgt gtgcgcgctg gatcatgcgt cgcgggtgga gcgcctcgtg
     5041 ctggtcgggg cggtgtcgaa tgatgacgta ccgcgtcggg tgtggctgcg gctggccgcg
     5101 tcgcccgtcg tcggggaagt gatctcgccc gtcctgctcg acgcgcggcg tctggtgaaa
     5161 cgtcgcctgc gtcgaactta cgcggcgcag aacaatctcc tgttcgacga ggaacgcatc
     5221 acggcgcacc aacgcccgct gctcgcggcc aacacgcacc gggcgatcct gcgcacgctt
     5281 cgtagctgga gcgcggagcg catcgaactc gaagccgcgc gcatcacaca gccgacgctg
     5341 ctcgtgtggg gcgcgcacga cagtgaagtg ccgctcagtt cgggcaaacg cctgcacgaa
     5401 ttgatcgaag gttcgcgctt gctcgtcttt cccaattgcg gccacctgcc gcaggaggaa
     5461 cgcccacggg aattcgcttc gctcgtcgcg gagttttgcc gggagacgcg cgggacggac
     5521 gaggcttccg ggggcgcatg agaggagttt gagaaatcat gtctgttcag cgtaacgatt
     5581 ggtctttgca acgcaagggc atcatcgatc aggagcggca caaggagcgc gtcaaggatg
     5641 cgatccgcaa aaacctgggt tccatcgtct cgaacgaagc gatcatcttg tccgacggca
     5701 agcgcagcgt gaaagtcccc atccgctcgc tcgacgagta caagtttcgt ttcgatcatc
     5761 gcaagcgcaa gcacgtcggg cagggcgacg gcaagacgcg cgtgggcgac gtgctggcgc
     5821 gcgaacaggg acaggggcag ggcagcggca gcggacccgc cgggcaggag acaggcgcgg
     5881 actactacga agccgaagtc aacatcgacg aactggccgc gctcatcttc gaagacctcc
     5941 atttaccgtt cctcgaagag aaggcgaaga aggccgtccc cgcgaagacg acgcgcttca
     6001 acgagattcg acgcacgggc gtgatggcga atctcgataa acgacggctc atccttgaga
     6061 acatcaaacg caacgcgcgc gaggcgaagg gcgcgaaatt cggcaacgtc aagaaagagg
     6121 atttgcgttt caaaagttgg gaagaagagc atcgctacga gtcgaacgcc gtcgtgctcg
     6181 cgctgatgga cgtgtcgggt tcgatgggcg agtttaaaaa gtatatcgcg cgctcattct
     6241 tcttctggat ggtgcgtttc ctgcgcacga agtacgacaa cgtggagatc gtcttcatct
     6301 cgcatcatac ggaggcgaag gaggtgtcgg aggagcagtt tttcacgcag ggcgaatcgg
     6361 gcggcacggt cgtctcgtcg gcctatgccc ttgcgctcga catcatcgcc gcgcgctaca
     6421 acccgcgcga ctggaacgtc tacccgtttc acttctccga cggcgacaac tattactcgg
     6481 acaacgaaga ggccgtgcgc ctcgcagacg agttgatcca gacctgcaac ctcttcggct
     6541 acggcgagat cggcgaagag ggcacttccg gttatcgtcg ctcgtcgggc gcgctgctct
     6601 cgatcttcaa agaccgcctg caacgcaagg aacgcttcgt cggcgttcgc atagacgagc
     6661 gcgaagatgt ctatcccgcg ctcaaacagt tcttcgggca gcgcggcgta gaagcctgac
     6721 gccttttgcg ccgttacgac ccgaacgctt gcgcccggcg cgcgctgcaa cctctctctg
     6781 cgccgcccct cgcccgactt tccctgcgcc cttcgctgct cgacgcagcg acgcttgaag
     6841 tttacgaaaa aataggcttg agcgtcgggc gcgaatttca taaatctaaa gcgttatcca
     6901 aatcccgcaa gattaactcc gcccgcagtc ccgcacggcc acagcctatc tccttatttt
     6961 ataaatagat caattttaaa tttatcaacg gcacaacgct tgcgagtccg tctcgacaag
     7021 aagcaagacg ctgcgagtcg ttcattcgca aacctgtaac ctccagttta tttggacggc
     7081 aggagtcttc cccgagatga gcgcaacgct tcacagggtc ttgcaggccg cgtcgcgggg
     7141 aatcagccgc gcccggcgct aacggttgga cgcacaatcc aacggtaaag gagtcttaaa
     7201 atcccatgaa caacagattc aagaaatggc tggcgacttt tctctcgctg tctatgatca
     7261 tgttgagttt gtccgtgccc gccggggcgc aggaaccgac gggcgctatc gaaggcaccg
     7321 tgaccgaccc gcagaaggcc atcgtcttaa acgcgaccgt cacggcgcgc aacaccgata
     7381 ccaatttgag ccgcactgtg accacgggcg acgacggtaa tttctccatc cgccagttgc
     7441 ctccgggcaa ctacgaggtc acgatcacga gcgcaaactt taagaagtct gtctataaag
     7501 acgtgacggt tgcggtcggc cagaacgcct cgctcgatgc ggagttgcag gtcggcgggg
     7561 cgaccgaaga ggtcaccatc gtcggcacca gcgaagcgca gattgatcgc gtagaccaca
     7621 acgtctccgg cgtcgtcggc accgtgcaga ttcaaaatct gcctctgaac ggtcgcaact
     7681 tcctcgatct cgcgcaactc caacccggcg tcgagaaggt cgagggcggc agcttcgacc
     7741 cgaccaaagc caactacacg ggcatctcca tcgccggtca ggccggacgc tccacgcaga
     7801 tcgccgtcga tggcggctcg gtcgtggata acgtcgtcgg cacgaccgtg cagaacttct
     7861 cgcaggagat cgtgcaggag tttcaactcg gcatctccaa ctacgatctt tcgaccggcg
     7921 catcctcgac cggctccgtg aacgtgatct cgaaatcagg ctcgaacgat tttcacggca
     7981 acgcctacat ctatgcgcgc gacgataagt ggtcggcctt cccttcgctc ggcaggctcg
     8041 acgccgcgaa caatattccg gcgacagctc aggccacccg cattcccttc gaccggcagc
     8101 agtacggctt cacgctcggc ggccccatca atcgagacag gctgttcttc ttcttcaact
     8161 acgaagggaa caatcaggat ggctccgcgc tgcacaaccc gaccgaagcc ccttcgttcg
     8221 ccggtttcac cgcgaacccg ttcgatgaaa agctgttgac cggaaagatc gactggatcg
     8281 tcagcaacaa gacgaacatc tccacgcgct actcgttcaa cgacaacttt caggatgtgc
     8341 cgttcgcgcc cggctccggc atcgtgccgc gcgcctcggc ctcgcgcatc ttcgagtcga
     8401 acaaccaact cgtgtcgaac cgctcgcaca atgtcgtcat cggcacgacg cacgcgttca
     8461 gcccgtccgt gaccaacaac ttcgtcgtca acatcagtga tttctcgaac gagatcgatc
     8521 cggcggtcga gggcgtaccc gaaatccgca tcctcggctc gcagttgttc cgcaccggca
     8581 ccaacgccat cgcgccgcag agcacctatc aggagcgcca ccagttgcgc gacgacctga
     8641 cgatgattcg cggcaatcac acgttccgca tcggcggcaa ctacgagcgc acttccatca
     8701 acgggacgtt cgccttcgcc aagcccgcgc gcatacgtct cttcaacccg gcagacctcg
     8761 aacacccgct gccgccctac cagacggagg ccgaattcct gaacgcgccc gtgcgcgaca
     8821 tctcgatggg cattggcagc gacgtgctgc ccttcaactc cgaccgcagc gacacgctca
     8881 accaccgctt ccagttctac ggcaacgaca cgtggaaact cgccaaccgg ttcacgatga
     8941 acttcggcct gtcgtatcgc atcgactcga acctgtggaa ccacgaccag ccgcgcccgg
     9001 ccatcgtctc ctcgctcttc ggcaagggca cggcggccag tccgcgcgac aagaacaaca
     9061 tcgccccgcg cgtcggcttc gcgtgggacg tagccggtaa cggcaagacg gtcgtgcgcg
     9121 gcggcatcgg catctactac gacaacacca tcgacaacct gcgcctcttc gagcgcgccg
     9181 atctcggtcg ccccggagcg gagttgttcc tcgtcggcgt tgacctccgc agcccgctct
     9241 tccccggcgg cgatggccgc ttcagcgagg gtcagttgac gctcgctcag gcgctcgccc
     9301 tcgtgggtcc ggcgcgcgcg gatatcgagt cgcgccagtt caactgcccg gcaggccaga
     9361 cctcgctcga atgcgccgaa gcgatctccg gaccactctt ctcgaccgag ttccaggtgc
     9421 cttactcgat ccagtacgcc gtcggcgtcc agcgcgagtt gccgtggaaa atgctcttcc
     9481 aggccgactt caactatcgc aaggggctgc acgaagtcat cgtctatgac atcaacaaga
     9541 ttgacgacga catcaccggc ccccgctcgg ccttcccgaa caccgtgcct tacgccgact
     9601 cgtccggctt ctcaacctac atggccggtc tgttccgcct cgaccgtcgc ttctcaaacg
     9661 gcttccagat gacggcgagc tattcgctct cgcgcttcaa agccttcggc ggcgacgccc
     9721 tcggcctcgg cgcgaccgtc accgacctca acaacttcag gccggagttt ggtcccgccg
     9781 gtctggaccg cacgcaccgc ttcgtcgtga gcgcgatcta cgaactgcct ttcttccgca
     9841 cgagcagcaa cgccttcaag cgcaacgtgc tcggcggctg gcaggtttcc atgatctcga
     9901 cggcgttcag cggcatcccg cagagcgtct tcctgccgca cttcgcagac ctctcgcgca
     9961 cgggcacgtt ccagacctac ctgcccggca cgcgcaacgg ctccatcggg cgcgacatca
    10021 ggaacgtggc cgacctcaac tcgctcatca cggcttacaa cgcgagcatc cccacgctcg
    10081 gcgtggattg cggccttgag aacagcccga ccgggcgttg cgacagcacc cacccgtcct
    10141 acttcgaccc gctcacgcgc ctcgccctcc tgcccgccga tacgcaactc ggcggcgact
    10201 cgatcatctc gcaggatgtg cgtctgacca agacgttcag attcggcgag cgttcgagcc
    10261 tcgacttgat cggagaggtc ttcaacctct tcaacgtcgc caacctgacc tacccggcgg
    10321 ccatcgccct ctccgacgag ggcacgtccg cgagcgacgt gaggaacgtc acgcacgggg
    10381 cgagcgcgcg taccacgagc gtcttcggca cgggcggacc acgcgccttc cagttcgccg
    10441 cgaagttccg cttctaagcg aacgcctccg tttgtttgcc tctggtggag cctgaaacat
    10501 ggctccacca ttttttcatc gtcgcctcgg aagtcgccgc agaaacgggc gacccggcga
    10561 cgcgttctac cttcgagtta tcttccttag agaggattcc cgccatgcgt caacgctcag
    10621 ccctgctcgc gctcactttc actctctgct gttacttctt cttctgcgct gccgccacgt
    10681 cggcgcacgc tccgcaaggc cacaccattc gcggcaaagt gcgcagttcg gcagggttca
    10741 acgtgccgcg cgtgacggtc aatttggaga gcggcggcgg cgcgttgatc gatcaaacgg
    10801 ttactaacaa cgaaggcgat ttcttcttcg gcgggctgga caacacgagt taccagatta
    10861 ctgtctcggc tcccgactac aatccggcgt ccgagcgtgt ggagttcgtg cgcaacgtcg
    10921 gcgcgagcga acccggcgag acgcgcacgg tc
//
LOCUS       DASWNS010000485         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_407458, whole genome shotgun sequence.
ACCESSION   DASWNS010000485 DASWNS010000000
VERSION     DASWNS010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407458"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VGB76_16645"
     CDS             complement(<1..598)
                     /locus_tag="VGB76_16645"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0100577.1"
                     /translation="MTSSKYLIAGIVLLIIVGVVGTMTAYANIDYAQEGTVKVVTYFG
                     RIERVYTPSDGWFTTLAPGRDSHEVNIRSFTESAPVRVTSKDNAALNVNIDVTAFTDA
                     TKVEEYVRKYSFDEAERHKRRNEILKGLLQTEARNAFSEFNAYEIYANQEQIQKRIVD
                     TLKPQLASQLQLVMESVQIGNPDFLDDRIEAAASEVVAN"
     gene            912..>1770
                     /locus_tag="VGB76_16650"
     CDS             912..>1770
                     /locus_tag="VGB76_16650"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0100578.1"
                     /translation="MKVYPTKQIRNLAVIGHGDAGKTQLISSLLYVTGATPRWGKVAE
                     GTTVTDYEEDSIARQITLNTALAHLEFREHKINFIDTPGYAAFVAHARPALRVADCAL
                     VVVDGVNGVEVQTEKTWAYANEFIVPRMMVINKLDKERADFGAALDSARHTFSRAIVP
                     FTLPIGKEADFKGVVDIVHMKAYEFDEQGKAKERAIPEVGRDVIDKERERLIEVIAES
                     DDALMEKYFENGTLEEADIIPNISKAIAASKLCPVFACSSLTLVGLQNLLEGIIDYAP
                     APDTHEAEHG"
BASE COUNT          365 a          573 c          454 g          378 t
ORIGIN      
        1 cgttcgccac cacttcggaa gccgccgctt cgatgcggtc gtcgagaaaa tccgggttgc
       61 cgatctgcac gctctccatc acgagttgca gttggctggc gagttgcggc ttgagcgtgt
      121 ccacgatgcg cttctgaatc tgttcctgat tcgcgtagat ctcgtaggcg ttaaactccg
      181 agaaggcgtt gcgcgcttcg gtctgcaaca agcctttcaa aatctcgttg cgccgcttgt
      241 gccgctccgc ctcgtcgaaa ctgtacttgc gcacgtactc ctcaaccttc gtggcatccg
      301 taaaagccgt cacgtcgatg ttgacgttca aggccgcgtt gtctttgctc gtcacgcgca
      361 cgggcgcgct ctccgtgaac gacctgatgt tcacttcatg gctgtccctg cccggcgcga
      421 gcgtcgtaaa ccaaccgtcg gacggcgtat aaactcgctc gatgcggcca aagtaggtta
      481 ccaccttcac cgtcccttcc tgcgcatagt caatgttggc gtatgccgtc atcgtcccga
      541 ccacacccac gataatcagt agcacaatcc ccgcgatgag gtatttgctg ctcgtcatag
      601 agtctccttt tttaatgtct acaatttccg gccaaatgtt cttgtctttt ttcatctcac
      661 ttcataatac gatccaaatc tgtggaagtt tgttaatttc agattaacgc gcgattcaag
      721 tgcgcgctgg tggcttccgg ctcgccctca cacggcgcac gtccgcttgc tttcccccca
      781 acgctcggat aaatttgctg cacacccgga gcgcgatgcc gcaactcttt gaggaaacct
      841 gctcggccac cagcggctcg ctttttctcg gcgcaatcaa atccctctga aaggatgctt
      901 ttcttccatc tatgaaagtt tatccgacca aacagattcg caacctggcc gtcatcggcc
      961 acggcgacgc gggcaaaacc caacttatca gttccctgct ctacgtcacg ggcgcaaccc
     1021 cgcgctgggg caaagtcgcc gaaggcacga ccgtcaccga ctacgaagag gactccatcg
     1081 cgcgccagat cactctgaac accgcgctcg cccatctcga attccgcgaa cacaagatca
     1141 acttcataga cacgcccggt tacgccgcct tcgtcgcaca cgcgcgcccc gccctgcgcg
     1201 tcgccgattg cgcgctcgtc gtcgttgacg gcgtgaacgg cgtcgaagtg cagacggaga
     1261 agacctgggc ttacgccaac gagttcatcg tgccgcgcat gatggtcatc aacaagctcg
     1321 acaaggagcg cgcggatttc ggcgcggcct tagacagcgc acgccacact ttcagccgcg
     1381 ccatcgtccc cttcacgctc cccatcggca aagaggccga cttcaaaggc gtcgtggaca
     1441 tcgtccacat gaaggcttac gagtttgacg aacagggcaa ggccaaagaa cgcgcgattc
     1501 ccgaagttgg ccgcgacgtg attgataagg agcgcgaacg cttgatcgaa gtcatcgccg
     1561 aatccgacga tgccctgatg gagaagtatt tcgagaacgg cacgctcgaa gaagcggaca
     1621 tcatcccgaa catctcgaag gccatcgccg cgagcaagct gtgtccggtc tttgcctgtt
     1681 cgagcctcac gctggtcggc ctgcaaaacc tgctcgaagg catcatcgac tacgcgcccg
     1741 ctcccgacac gcacgaagcc gagcacgggc
//
LOCUS       DASWNS010000486         9997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_122852, whole genome shotgun sequence.
ACCESSION   DASWNS010000486 DASWNS010000000
VERSION     DASWNS010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9997
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122852"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1043)
                     /locus_tag="VGB76_16655"
     CDS             complement(9..1043)
                     /locus_tag="VGB76_16655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0100579.1"
                     /translation="MPDSLLTEDASRPDGYERSSGDYKIWQVIAASAVGTMIEWYDFY
                     IFGSLAAVISPLFYPPGNDTFAYIAYLATFAVGFIVRPFGALFFGRIGDIVGRKYAFL
                     VTLLIMGGATAVIGFLPTYSQIGIAAPVILLFIRVLQGLALGGEYGGAAVYVAEHVPD
                     RRRGFYTSFIQITATLGLFVSLAVILIVQNMMSADAFAGRTGSFAGWRIPFIISIFLV
                     GVSLYIRLRMKESPIFQHVKSSGMTSAKPLTEAFTKWSNLKLVLISLFGATAGQGVVW
                     YTGQFYALFYLQSILNVDATSANYIVAIALLLGMPFFVVFGMLSDRVGRKWIIMAGCL
                     LAVITYIPIY"
     gene            1510..2400
                     /locus_tag="VGB76_16660"
     CDS             1510..2400
                     /locus_tag="VGB76_16660"
                     /inference="COORDINATES: protein motif:HMM:NF017437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0100580.1"
                     /translation="MKAYSFYGLSENCFAPSPNPRYLHLTDQTNACLFKCRFVIDERQ
                     GLSLIVGKVGYGKTSVLRELVSGYVDDDTNKIVMLPNGNFPTEMQLAKAIAAELGLPA
                     RRSLDAQKMEIRQFAMRTYGEGGNVLLIIDEAQNLFGPLFDFLRELLNYETDESKVIQ
                     IIIAGQPEIETKLATKQALVSRIILTNYLDTFTYDDMLQALEHRVRVAGGGKKLSAVI
                     DEAGCKAVYLASKGVPREIMKIVNASMLLGALNQVKPITEDLVKVAVENVRLSEKDHG
                     REENNELRTGAQNQAGVAAT"
     gene            2333..3451
                     /locus_tag="VGB76_16665"
     CDS             2333..3451
                     /locus_tag="VGB76_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100581.1"
                     /translation="MAEKKIMNFAQALKIRPGSLRPKPEVQPVEEDNSTSLVPLPPNP
                     ESASDNTATTSTPTPTTTSTPTTTSTPKTEAEHPIAPTRDFTRVANSIVRKAVASGVF
                     SGKSKQVYDYLYSQTRGAITPRRSFRIPMDKLRVNSGLGSDKSLRSILERLAASGLVT
                     IRQITGTQGGNEFTVYLPEEIGTPTTTSTPTPTTTGGRNDHLYPSYKVPGVEVVETTS
                     GSRGLSIVESTTSDDAKTSFKTNANTSDDDAGAQRLTQKLLQTERELTGKVSTSTERW
                     DELAELLATELRIAAARTTVSNVPAFLTEHLRRRLWKVDEQRASEIAADSGEGTHTPA
                     LSEEERRKCPDCAGTGFWYPEGTEKGVAKCLHQKLKSK"
     gene            3807..4679
                     /locus_tag="VGB76_16670"
     CDS             3807..4679
                     /locus_tag="VGB76_16670"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0100582.1"
                     /translation="MSEATRRAYSRAVADFFRFARGKHPAEVVPADVQLWRDHLRSQR
                     KRPATVSFKLSVIRSFFEYLKAAGVILLNPASTKLVAPPELPTEPSGRALTAKEVRYL
                     LSGPDREKAEGARDYALMLVMLRLSLRVSEVCSLRASSVKWSHGRWTLRCKVKGGREE
                     TWPLPKDVKEAVDHYLRIDRKRREVSHSDGEDAPLFQPHVNYRTLEFDKGISTRMVQK
                     IVKRWADYSNLGDLSPHDLRRTAITKALDSGLTYRQVQMMSKHKDPKTVMRYDHGREN
                     LDQNAVNFLKYDEE"
     gene            4986..5681
                     /locus_tag="VGB76_16675"
     CDS             4986..5681
                     /locus_tag="VGB76_16675"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0100583.1"
                     /translation="MTFSYKYERPGLTVDCVIFGLDLEEERLKVMLIERNLEPFAGMW
                     AIPGGFVHQGETLEVAATRELREETGITDVFLEQLYTFGDLDRDPRGWVVSVAYYALV
                     SPEKHTIHAASDARQARWFPVNSLPRLAFDHAEILRTALERVRGKLTYAPIGFELLPR
                     KFTIKQLQKLYEIVLGTKLDNRNFRKKIFGMDVLQELDEMQQGVPHRAARLYKFDERK
                     YQQLIKRGLSFGL"
     gene            5698..6255
                     /locus_tag="VGB76_16680"
     CDS             5698..6255
                     /locus_tag="VGB76_16680"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002014.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HEY0100584.1"
                     /translation="MKDRNTRASKFLSLVLRHQPEKIGLTLDREGWVAVSRLLNALAA
                     HGLDLTPYELQEIVRTNDKQRFSLSPDGLFIRANQGHSVEVELGYESAAPPPVLYHGT
                     AERFLSSIKEQGLLRGRRHHVHLSEQQETATAVGRRYGKPVVLRIACDEMHAEGHLFF
                     RSANGVWLTEHVPPRYITLREDDRS"
     gene            6324..7127
                     /locus_tag="VGB76_16685"
     CDS             6324..7127
                     /locus_tag="VGB76_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100585.1"
                     /translation="MSKRIELLIIDPQVDFCDPQRGALYVPGAEHDMGRLASMIRRLK
                     HKLDDIHVTLDSHHFIHIAHPIFWKDSHGAHPPAFTRISRADVEGGVWTPTVPGMYKR
                     ALEYVRRLEQNGRYELTVWPPHCLIGSPGHAVFHGLFAALTEWESRFAFVDYVTKGSN
                     ILTEHYSAVQADVPDSSDASTQINTRLIQTLENADVIAIAGEARTHCLANTVRDIANN
                     FGDDSFVSKLVLLTDASSDIPGFESHARDFMSEMTRRGMQLSTTTEFLV"
     gene            7146..7889
                     /locus_tag="VGB76_16690"
     CDS             7146..7889
                     /locus_tag="VGB76_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100586.1"
                     /translation="MPILNDTTLDQITLPDSHYGYSATRLDELGATEYTVVTIACDVS
                     GSTTAFIFDMESAITRIVQACKSSPRADNLLLRLVAFDDTLSELHGFKLLESCHLADY
                     GGILRAGGSTALYDAAENAVSSTINYGHQLLSGDFSANAILFVITDGMDNASRLPAKK
                     VKEALARAITSEALESIVSVLIGVNVQDRQAGKFLRQFQVEAGFTQYVELDKADAKTL
                     ARLAEFVSRSISAQSQALGTGGASQSLVF"
     gene            7983..8861
                     /locus_tag="VGB76_16695"
     CDS             7983..8861
                     /locus_tag="VGB76_16695"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY0100587.1"
                     /translation="MNADSAFQIGATHAVCQDYSLAGGVLPAALGEGFAPPQPSRRPY
                     VILSDGCSSSPDTDIGARLLVKAAEQMLPGRGSATTLALAEIHREAARLALEWAELLG
                     LPPQAVDATLLSAHLDGEELVVGCSGDGVICLQTWAGAVEVYSISYASGYPLYPAYTH
                     QPARLLALKEAGHSGKEVTRFRAASIEECLQPSGTSGGDSLTEVFTVSAANYKYVALF
                     SDGIHSFFNAGQNGSSGSRGEAVPLDEPLRSLVSFKSTRGAFVGRRMKRFLKDCRNRG
                     WQHLDDLAFGALHLGG"
     gene            8864..>9997
                     /locus_tag="VGB76_16700"
     CDS             8864..>9997
                     /locus_tag="VGB76_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100588.1"
                     /translation="MSFKSVFPGLSTFLKGDDATMDRYFVQGRGEVRLSKSDFKAQGG
                     EGSIYLKGSNAYKIYTDPARAIPPAKILELSALTLPNIIRPLDVVLDAKNRPVGYSMR
                     SVGRSYALCQLFPKAFRQRNNLTPELSLGLVRKLQAGVSHVHARAILIVDLNEMNFLV
                     AEDFAELFFIDVDSYQTPSFPATVLMESVRDRHAQRLTTESDWFSFAVVSFQLFVGIH
                     PYKGNYPPFQGLPDKEKKLDARMRANVSVLRPEVSVPASCLPFSVIPPAYLDWYRAVF
                     EEGKRLPPPDNTQPALTLAAPQPPRAVGSAHFEFTEIREFDTGVNWHDGLITITQKSI
                     YVQGEQFPKPQADVLVTTTPRLRHLVAAYMNGAAPCIRDLTESR"
BASE COUNT         2283 a         2917 c         2780 g         2017 t
ORIGIN      
        1 tgaatacctt aatagatggg tatgtaagtg atgacggcca gcaggcaacc cgccatgatg
       61 atccacttcc gccccactcg atccgagagc atcccgaaga cgacgaagaa cggcatgccc
      121 aagagcagcg cgatggcgac aatatagttg gccgaagtgg cgtccacgtt gagaattgat
      181 tgcaggtaaa aaagcgcgta aaactgcccc gtgtaccaga ccacgccctg ccctgcggtt
      241 gcgccgaaca gagagatcag cacgagtttc aaattactcc atttggtaaa ggcttctgtg
      301 agcggtttcg ccgaagtcat gccggagctt ttgacgtgct ggaaaatggg cgactctttc
      361 attcgcagtc gaatatagag cgagacgccg accagaaaga tcgagatgat gaaagggatg
      421 cgccagccgg cgaacgatcc ggtacgcccg gcgaaagcgt cggcgctcat catgttctgc
      481 acgatgagga tgacggccag tgagacgaag agtccgagcg tcgccgtgat ctggatgaaa
      541 ctcgtataga aaccgcgcct gcggtcaggc acgtgctcgg cgacatagac ggccgcgccc
      601 ccatactcgc cgccgagtgc cagaccttgc aggacgcgta tgaacaggag gatgacgggg
      661 gcagcgatac cgatttgcga gtaggtcggc aaaaagccga taacggcggt tgccccaccc
      721 atgatcagca gcgtgacgag aaaagcgtac ttccgtccca cgatgtcgcc gatgcgcccg
      781 aagaagagcg cgccgaaggg gcggacgata aagccgacgg cgaacgtagc gagataggcg
      841 atgtaagcga aggtgtcatt gcccggcgga taaaagagcg gagagataac cgcggcgagg
      901 ctgccgaaaa tatagaagtc gtaccattcg atcatcgtgc cgacagcgga ggcggcgatg
      961 acctgccaga ttttatagtc gccggaactc ctctcatagc cgtcggggcg gctcgcatct
     1021 tctgttaata aactatcagg catcaagtcc ggtgctcctt aatcaatatg gttccggtgc
     1081 agaccgagat gtgagcagat aatcttcacg tcgctccact cgctcccaac gcagccttac
     1141 tatattgaag gtcgccactt taatcattaa taatcgtccg cagtatcaga tagtacatca
     1201 cttacaaact cggctactgc gactttggtg ggaatgatgg ccgaacgaga gattgctctc
     1261 tctcacgcga tcatccagct attggttcag cgtcggaatc gcattatgtg gcgacagttg
     1321 agcggtgtgg tcgtgcgtgg cttggtcaga acgttgtctg cgattaagct gagcagtcgt
     1381 ttcctccgca acaatcaaca aatccttgta gctgcacacc cttatgtcct aagctggctt
     1441 caattcccgc ccccctctac cgaaattatc attgacaaga ccagtcccct agccttataa
     1501 cgctccagta tgaaagcata cagcttctat gggctgagcg aaaactgttt cgcacctagt
     1561 cctaacccgc gctatctaca cctgacggat cagaccaacg cctgcctgtt caaatgcaga
     1621 ttcgtcattg atgaacggca ggggttgtcg ctcatcgtcg gcaaggtcgg gtacgggaaa
     1681 accagcgtgc tccgcgagtt ggtgagcggc tacgtcgatg acgacaccaa caaaatcgtc
     1741 atgctgccca acggcaactt ccctaccgag atgcagttgg ctaaagcaat agcggctgag
     1801 cttggcttac ccgcccgccg ttcgttggac gcacagaaga tggaaataag gcagttcgca
     1861 atgcggactt acggcgaggg cggtaacgtc cttttgatca tcgacgaagc gcagaacctc
     1921 ttcgggccgc tattcgattt ccttcgcgaa ctccttaatt acgaaacgga tgaatccaag
     1981 gtgatccaga tcattatcgc cggtcagccg gagattgaaa ccaagctagc gacaaagcag
     2041 gcactcgtca gccgcatcat cctgaccaac tatctcgaca ctttcaccta tgacgacatg
     2101 ctccaggcac ttgaacatcg agtgagagtt gcgggcgggg ggaaaaaact gagtgccgtt
     2161 atcgatgagg cgggctgcaa agccgtctat ttggcctcca agggagtgcc acgggagatc
     2221 atgaagatcg ttaacgcatc aatgctcctc ggtgccctga atcaggtgaa gcctattacg
     2281 gaagacctcg tcaaggtcgc agtcgaaaac gtcagattgt cggagaagga tcatggcaga
     2341 gaagaaaata atgaacttcg cacaggcgct caaaatcagg ccggggtcgc tgcgacctaa
     2401 gccggaagtc caacctgtcg aagaagataa cagcactagc ctggttcccc ttccgccaaa
     2461 tccggaatcg gcgagcgaca acaccgctac cacctctacc cctaccccta ctaccacctc
     2521 tacccctact accacctcta cccccaaaac ggaggccgaa catcctattg cgccaacgcg
     2581 tgacttcacg cgggttgcca attcgattgt gagaaaggct gtcgcttccg gtgtcttctc
     2641 gggaaaaagc aagcaggttt atgattacct ctatagtcag acaagagggg cgatcacgcc
     2701 ccgtcgttcc ttcaggattc cgatggataa acttcgggtc aattccgggc tagggtctga
     2761 caaatcccta cgctcaatct tggagcgttt agctgcgtca ggcttggtca ctatcagaca
     2821 gattactggc acccagggcg gcaacgagtt tacggtatac ctgccggaag agattggcac
     2881 ccccactacc acctctaccc ctacccctac taccacaggt ggtagaaacg accacctcta
     2941 cccctcctac aaagtaccag gggtagaggt ggtagaaaca accagtggta gtaggggttt
     3001 atctatagta gaatcaacaa cttccgacga tgctaagact tcttttaaga ctaatgcaaa
     3061 tacatctgat gatgacgctg gcgcgcagcg tttgactcaa aagctgctcc agaccgagag
     3121 ggaattgacg ggtaaagtct cgacgagtac cgagcgctgg gacgaactgg ctgaactcct
     3181 cgccaccgaa ctcaggatcg cggcggcgcg cactaccgtc tcgaatgtgc ctgcgttttt
     3241 aaccgagcac ctaaggcgaa ggctttggaa agtcgatgag cagcgggcgt ccgagatcgc
     3301 cgctgattct ggagagggaa cccacacgcc tgccctcagc gaggaagaga gacggaaatg
     3361 ccctgactgc gcgggcacag gcttttggta cccggaggga acggagaagg gcgtcgccaa
     3421 gtgcttacac cagaagctaa aatccaaata aggagacttc tcaaaagaaa gtttgtctcg
     3481 cattactgac ccaatgacca ctgaaaaggc accttctaat tccgtttcac gtgatgttac
     3541 acgaccacta atttagctgt atctccaatt aaaaatcgtt gtttgaggtg gcgagagcta
     3601 attccgataa ttaggctttt acgaaagaag tgcttgacta cgtttgcttc ttaagtcatc
     3661 ataaagttga ttttgcccct taaattggcg ctgaagggtg tttctacttg ccgactgaaa
     3721 cattaagtcc ggaaatcttt agcgggcggg agatcgctcc gagaaagttt gacccgagaa
     3781 gtgccgctcc cttcgtcaat aagtccgtgt cggaagcgac ccgtcgtgcc tacagtcggg
     3841 cggtagccga cttcttccgc ttcgcacggg gcaagcaccc ggcggaggtt gttcctgctg
     3901 atgtgcaact ctggcgcgat catttgcgct cgcagcggaa acgcccggcc actgtctcct
     3961 ttaaactctc cgtcattcgc tccttcttcg aatatctgaa agccgcaggc gtcattctgc
     4021 tcaatcccgc ttcgacgaaa ctggttgcgc cgccggaact gcccacagaa ccttcagggc
     4081 gtgcgctcac cgcaaaagag gtgcgttacc tgctgtcggg gccggatcgg gagaaggcgg
     4141 aaggagcgag ggattatgcg ctgatgctcg tcatgctccg cctctccttg cgtgtctcgg
     4201 aggtttgcag cttacgcgct tcgtccgtga agtggagtca cgggcggtgg acgctgcggt
     4261 gcaaagtcaa aggggggagg gaagagactt ggccgctgcc caaagacgtg aaggaagccg
     4321 ttgatcatta tctccgtatc gaccgcaagc gcagggaagt ctcacattcg gacggggaag
     4381 atgcgccgct cttccagccg cacgtcaact accgcacgct ggagtttgat aaaggcatct
     4441 cgacgaggat ggtgcagaag atcgtcaaac gatgggctga ttactccaat ctcggcgacc
     4501 tctcaccaca tgacctgaga cggacggcga ttacgaaggc gttagactcc gggctgactt
     4561 acagacaggt gcagatgatg agtaaacata aagacccgaa gaccgtgatg cgttatgacc
     4621 acgggagaga gaatctagat cagaacgccg tcaacttcct taagtatgac gaggaataga
     4681 atagtctgcg gaaaatgacc gtttgtcatg gcggattgcg atagggacgc cctcgccggg
     4741 tgcttagact aggcgctcgg gtggccgtac agactaggac tcctgcatta tctccacaac
     4801 ctcacgataa aaaagttaac cgctccagac attttcttct tgacattgag aaggctttgc
     4861 gtattatgga cgctaaggat agcgttgtat gaacgttatc gcatgtgagg tttttggtaa
     4921 tcgtagaccg ctttccggct cgggcgataa gtccctgcca caggtcagaa tgcggagtga
     4981 ggacgatgac ttttagctac aagtatgaaa ggccgggtct gacggtcgac tgcgtgatct
     5041 tcggacttga tcttgaggaa gagaggctga aagtcatgct cattgaaaga aaccttgagc
     5101 cttttgcggg gatgtgggcg atcccgggtg gcttcgtcca tcagggtgaa acgctcgaag
     5161 tggcggcgac gcgcgaactc cgtgaggaaa ccggtatcac ggatgtcttt ctcgaacaac
     5221 tctacacgtt tggcgatctc gaccgtgacc cgcgtggatg ggtcgtctcc gtggcctact
     5281 acgcactggt ttctccggaa aaacatacga ttcacgcggc cagcgacgcg cgccaagcac
     5341 gctggttccc cgtcaactct ctcccacggc tcgcctttga ccacgccgag atactcagga
     5401 ctgcgcttga gagagtccgt ggaaagctaa cctatgcgcc catcggcttc gaactgctcc
     5461 cccgaaagtt cacgatcaaa cagctacaga aactctacga gatcgtgctc ggcacgaagt
     5521 tagacaaccg caatttcagg aagaagattt tcggcatgga tgtactacag gaactggacg
     5581 agatgcagca gggcgtgccg caccgcgccg cgcgtctcta caaatttgac gagcgcaagt
     5641 atcagcaatt gatcaagcgc ggcttgagtt tcgggcttta gtcggaaaga gcgaacgatg
     5701 aaagatcgca ataccagagc cagtaaattt ttgagcctcg tgctcaggca ccagccggag
     5761 aagatcggcc tgacgcttga ccgcgaaggt tgggtcgcag tctcacgtct gctcaatgct
     5821 ttagcggcgc acggcctaga cctgacgccg tatgagttgc aagagatcgt ccgcaccaac
     5881 gacaagcagc gtttctcgct cagtccagac ggcctgttca tcagagccaa tcaggggcac
     5941 tctgtggagg tagaactggg ctacgaatcg gctgcgccgc ccccggtgct atatcacggg
     6001 acggcagagc ggtttctgtc atcaatcaaa gagcaggggc tgttgagagg ccgtagacat
     6061 cacgtacatc tctcggagca gcaggagacg gcgacggctg tcggcaggcg ttacggcaag
     6121 cctgtcgtcc tgagaattgc gtgcgacgaa atgcacgcgg aaggccatct gttcttccgc
     6181 tcagctaacg gggtctggct cacggagcac gtccccccgc gttacatcac actgcgcgag
     6241 gacgaccgga gttgatccat tttttttgcc ctcaagtctt agcgtcagtt ttacgctaag
     6301 ccgacacgaa ggagatctgt tcaatgagca agagaatcga actactaatc atcgacccgc
     6361 aggtggactt ctgcgacccg cagcgcggcg cgctctacgt gccgggcgcg gagcacgaca
     6421 tgggacggct ggcctcaatg atccggcgtc tcaagcataa gctcgacgac atccacgtca
     6481 cgctcgattc gcaccacttc atacacatcg cccacccgat cttctggaaa gactcgcacg
     6541 gggcgcaccc gcccgccttc acacgcatca gccgcgccga tgtcgaggga ggcgtctgga
     6601 ctccgaccgt gcccggcatg tacaagcgcg cgctcgaata cgtccgcagg cttgagcaga
     6661 acggacgcta cgagttgacc gtctggccgc cgcactgcct catcggcagc cccggccatg
     6721 cggtctttca cggcctcttc gccgcgctca cggaatggga gtcgcgcttc gccttcgtgg
     6781 attacgtcac caagggcagc aacatcctga ccgaacacta ctccgccgtg caggccgacg
     6841 tgcccgactc ttcggacgcc tccacgcaga tcaacacgcg cctcattcag acgttggaga
     6901 acgccgacgt gatcgccatc gcgggcgagg cgcgcacgca ttgtctcgca aacaccgtgc
     6961 gcgacatcgc caacaacttc ggcgacgact cgttcgtgtc aaagctcgtg ctgctcacgg
     7021 acgcttcgag cgacatcccc ggctttgaat cccacgcacg cgacttcatg agcgagatga
     7081 cccgtcgcgg catgcaactc tcaaccacga cggaatttct ggtctaaaga taaggagccg
     7141 agacgatgcc tatactcaac gacaccaccc tcgatcaaat tacactgccc gacagtcatt
     7201 acggctactc cgccacacgt ctggacgagt tgggcgcgac cgaatacacg gtcgtcacga
     7261 tagcctgcga cgtgagcggg agcaccaccg ccttcatctt cgacatggaa tcagccatca
     7321 cacgcatcgt gcaggcgtgc aagtccagcc cgcgcgcaga caacctgttg ctccgcctcg
     7381 tggctttcga cgacaccctc tccgaactcc acggcttcaa acttctggag agctgccatc
     7441 tcgccgatta cggcggcatc cttcgcgcgg gcggctcaac cgccctgtac gacgcggcgg
     7501 aaaacgccgt ctcctcgacc atcaactacg ggcaccagct cctatcaggg gatttctccg
     7561 ccaacgccat tctgttcgtc atcacggacg ggatggacaa cgcctcaagg ctgcccgcca
     7621 aaaaagttaa agaggctctg gcgcgagcca tcacgagcga ggctttggag tcaatcgtct
     7681 ccgtcctcat cggcgtcaac gtgcaagacc ggcaggcagg taagttccta cgacagtttc
     7741 aggtcgaggc gggcttcaca cagtacgtcg aactcgacaa ggccgacgcc aagacgctcg
     7801 cgcggctcgc ggaattcgtc tcccggagca tcagcgcaca gtcgcaggcg ctcggcacgg
     7861 gcggggcgtc acagtccctc gtcttctaaa caaagggctt cggcgggaga gcattaactc
     7921 gtgcgggctt gcttcgggtt aatgctcttc cgcccggctc tattcatcgg aggctctttc
     7981 atatgaatgc cgacagtgct tttcaaattg gcgcgacgca cgcggtctgt caggattatt
     8041 cgctcgccgg aggagtcctg ccagccgccc ttggagaagg gttcgccccg ccgcaaccgt
     8101 cgagacgccc ttacgtcatc ctgtctgacg gctgctcctc ttcgcccgac acggacatcg
     8161 gcgcgcggct tctggttaaa gccgccgaac agatgcttcc gggacgcggc agtgcgacga
     8221 cccttgcgtt ggcagagata cacagagagg cggcgcgtct cgccttggag tgggcggaac
     8281 tgctcggcct gcccccgcaa gctgtggacg cgaccctgct cagcgcgcat ctcgacggcg
     8341 aagagttggt cgtcggctgt tcgggagacg gagtgatctg cttgcagacg tgggcgggcg
     8401 cggtcgaagt ctacagcatc tcctacgcct ccggttaccc gctctacccg gcctacacgc
     8461 accagcccgc acggctgctc gcgctgaaag aggctggaca ctcaggcaaa gaggtgacac
     8521 gctttcgcgc cgcttcaatc gaagaatgct tgcaaccgtc gggcacgtca ggcggcgact
     8581 cgctaacgga ggtctttacc gtcagcgcag cgaactacaa atacgtcgcg ctcttctccg
     8641 acggcattca ttccttcttt aacgccgggc aaaacgggag cagtggcagc agaggcgaag
     8701 ctgtccctct ggacgagcct ctgcggagcc tcgtctcctt caaaagcaca cgcggcgcgt
     8761 tcgtcgggcg acgcatgaag aggttcctga aagactgccg gaatagaggg tggcaacacc
     8821 tggacgatct ggcgttcggc gcgctgcatc tcggaggttg agtatgtctt tcaaatcggt
     8881 ctttccgggt ctgagcacat ttctgaaagg ggatgatgcc acgatggaca ggtatttcgt
     8941 acagggtcgg ggcgaggttc ggctcagcaa gtcggacttc aaagcgcagg gcggcgaggg
     9001 ttcgatctac ctgaaaggct cgaacgctta taagatttat accgacccgg cgcgcgccat
     9061 cccacccgcg aagattctcg aactctccgc tcttacgctg cccaacatca tccgccctct
     9121 ggacgtagtg ctcgacgcga agaacaggcc ggtcggctat tccatgcgct ccgtcggcag
     9181 gtcttacgcg ctctgccagc ttttcccgaa agccttccgc cagcgcaata acctgacgcc
     9241 ggagttgtcg ctcggcctcg tgcgcaaact gcaagcgggc gtcagtcatg tccatgccag
     9301 agccattttg atcgtggact tgaacgagat gaacttttta gtcgcggagg atttcgcaga
     9361 gctcttcttc atagacgttg acagctacca gacgccttcg ttccctgcga cggtcttgat
     9421 ggagagcgtg cgagacaggc acgcgcaaag actgaccacc gagtccgact ggttctcctt
     9481 cgccgtcgtc tccttccagc tgttcgtcgg catccaccct tacaagggca actatcctcc
     9541 gtttcaggga ctacccgaca aagagaagaa actcgacgcg cggatgcgcg ccaacgtgtc
     9601 cgtgctcagg ccggaagttt ccgtcccggc ctcatgtctc cctttctccg tcatcccgcc
     9661 cgcctacctc gattggtaca gagccgtctt cgaagagggc aagcgactgc cgccgccgga
     9721 caatacacag cctgccctca cgctcgccgc gccgcagccg ccgcgcgccg tcgggagtgc
     9781 tcattttgag tttacggaga tcagagaatt cgataccggg gtcaactggc acgacggcct
     9841 catcacgatt actcaaaaga gcatctatgt tcagggcgag caatttccga agccgcaggc
     9901 cgatgtgctg gtaacgacga cgccgcgcct gcgccacctc gtcgccgctt atatgaacgg
     9961 ggctgcaccc tgcatccgcg acctgaccga gtcacgc
//
LOCUS       DASWNS010000487         4810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_96232, whole genome shotgun sequence.
ACCESSION   DASWNS010000487 DASWNS010000000
VERSION     DASWNS010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96232"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..86)
                     /locus_tag="VGB76_16705"
     tRNA            complement(10..86)
                     /locus_tag="VGB76_16705"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Pro,seq:cgg)
     gene            complement(131..808)
                     /locus_tag="VGB76_16710"
     CDS             complement(131..808)
                     /locus_tag="VGB76_16710"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY0100589.1"
                     /translation="MESLTELIHLVKQYLDPRTIAAAGYLVLIFVVFAETGLAAGFFL
                     PGDSLLVVAGLFAAKGDLNVFILLSTLFVAAVVGDAVGYYSGAKLGPRLFKRQKSLVF
                     RPSHLEKAQAFYEKYGGKTIIIARFVPIVRTFAPIVAGAAQMPYRKFVFFNVIGGFLW
                     VFSMILSGYFLGTLVERAFGIKLDEHIEKVVIVVVALSLLPPLFEYLKSRREKARLKS
                     VGDAPQV"
     gene            complement(878..1561)
                     /locus_tag="VGB76_16715"
     CDS             complement(878..1561)
                     /locus_tag="VGB76_16715"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY0100590.1"
                     /translation="MSSASQIPAAQASAFRRQRDQMVEHLRKHHGIGNERVLEAMREV
                     PRHYFVPEALQSNAYGDHALPIAANQTISQPYIVARMSELLELDNQSSVLEIGAGSGY
                     QTAVLARLAGRVLAIERISELARVAQARIRALNIYNATVKCFDGTLGWSAHAPYDAIL
                     AAAGGPDVPEPLMAQLKVGGRLVMPVGERRDAQRLVRVIRKEDKFIREDHGACAFVPL
                     IGRYGWSEQ"
     gene            complement(1601..2398)
                     /gene="surE"
                     /locus_tag="VGB76_16720"
     CDS             complement(1601..2398)
                     /gene="surE"
                     /locus_tag="VGB76_16720"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY0100591.1"
                     /translation="MIRILLTNDDGIHSDGLIKLEEALRAVGDVYTVAPASEMSGASH
                     SLTLSRPLRIRQIDERHWTVDGTPTDCVTLALHKILPREEWPHICVSGINHGGNLGDD
                     ASYSGTVAGALEATILGVTGLAFSLVARENYDFTYAAQFAIKIVKKVLAEGLPQGTLL
                     NINMPPGELRGVRITRQGLKNARPIITEKIDPRGKAYYWIGEEVFPEDTVEGTDYYAI
                     KQGMASITPMRSDMTDHRSLVELQNWNSPELEENVPCEEAGEPVEHT"
     gene            complement(2741..4783)
                     /locus_tag="VGB76_16725"
     CDS             complement(2741..4783)
                     /locus_tag="VGB76_16725"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0100592.1"
                     /translation="MRPIKHVERGLTALAAGAFNTIQFFNKYRPSESFIPKWSDKPLL
                     KSWQKSKPPLGFPRTTDSLCPKCVIEARAKILSAEVIDPAILINEKVGEVKAQIVERN
                     GEIWMIKDCPTHGHIEDIMAMDSTFLKHIEAQFPGRDIQAHNDEKLHNHGSSTIKYGR
                     GSVLTIDLTNRCNMMCDPCFMDANQVGFVHELSFDDVKDILDKAISIKPRRQMSVQFS
                     GGEPTISPHFIESIRYSRKVGYNSVQCATNGIEFAKSKEFCREAAEAGLRYAYLQFDG
                     IGNDANSHRQVGNLFDVKLRAIDNLHEAGVEIVLVTTLVNNINNDQVGSVIRFALDNP
                     KKVAFISFQPVSFTGRDEEITDERRMAQRYTLSHLAHDVKKQVGITEPTRDWFPLSLM
                     GAFADFADLVHGPEAEWGQVSCGCHPNCGVGTAVMINKNTKEMKPVPEFLNIPGLVKD
                     MTGITDAGRGKWFSNIMMGLALLKNYQPYGAPQSLTLVDIFKKFDKSFGLSGKSYGKV
                     EGDRTFEDIEKRRSDPWNFLFIAGMWFQDLFNYDFRRTEMCIIPYGTQEGEISFCAYN
                     TGIGWRNIIEQMHQNATIAKWYKEHGRHEIFAKGKNVELADAGFTGILNEVDLQRPNR
                     PEMAGPKTAAEEAAMMRKLYQEMVLKQKLGAGDVEKNTPVQIKGLGSKKEGKTISA"
BASE COUNT          958 a         1420 c         1392 g         1040 t
ORIGIN      
        1 gaagaaagat ggtcggggca agaggacttg aacctccgac ctcacggtcc cgaaccgtgc
       61 gctctaccag gctgagccac gccccgacaa acaaactgtg caggcattct aggtgcgacg
      121 tgccgacatg tcaaacttgc ggggcgtcgc cgacgctttt caggcgcgcc ttctcgcgtc
      181 tcgacttgag atattcgaag agcggcggca gaagcgacag cgcgacgacg acgatgacga
      241 ccttctcgat gtgttcgtcg agtttaatgc cgaaggcgcg ttcgacgaga gttccgagga
      301 aatatccgga gagaatcatg ctgaacaccc agaggaagcc gccgatgaca ttgaagaaca
      361 cgaacttccg gtagggcatt tgcgccgcgc ccgcgacgat gggcgcaaag gtgcgtacga
      421 ttggcacgaa ccgcgcgatg atgatcgtct tcccgccgta cttctcgtaa aacgcctgcg
      481 ccttctccag atggctcggg cgaaacacaa gagacttctg ccgcttgaac aggcggggtc
      541 ccagttttgc ccccgaataa taaccgaccg catcgcccac caccgccgcg acgaacagcg
      601 tgctgagtag aataaagacg ttcaagtcgc ctttagccgc gaacaatccc gccacgacca
      661 gcagcgaatc gcccggcaga aaaaagcccg cggccagtcc cgtctccgca aagacgacga
      721 agatcaagac cagataaccg gcggcagcaa tcgtgcgcgg gtcgagatat tgctttacca
      781 gatggataag ttctgtcagc gattccataa attaagtctc tagtcgcgcg tttcaagtct
      841 tgagtctcaa ttgtctccgg cttgaaatcc gggactacta ctgttccgac cagccgtagc
      901 gaccgatgag cgggacgaag gcgcacgcgc cgtgatcttc gcgtataaat ttgtcttctt
      961 tgcgaatgac gcgcacgagc ctctgcgcgt cgcgccgctc gccgacgggc atcacgagcc
     1021 gcccgcccac cttcaactgc gccatcaacg gttcgggcac atccggaccg cccgccgccg
     1081 cgaggatggc gtcgtagggt gcgtgcgcgc tccagccgag cgtgccgtcg aagcatttca
     1141 cggtggcgtt gtagatgttg agagcgcgga tgcgcgcctg cgccacgcgt gccagttcgc
     1201 tgatgcgttc gatggctaag actcgaccgg cgagccgcgc cagcaccgcc gtttgataac
     1261 cggagcctgc gccgatctcc agcaccgaac tttgattgtc gagttcgagc aactcggaca
     1321 tgcgcgcgac gatgtagggc tgcgagatcg tctggttcgc ggcgatgggg agcgcgtggt
     1381 cgccgtaggc gttgctctgc aaggcttcgg gcacgaagta gtggcgcggc acttcgcgca
     1441 tcgcttcgag cacgcgctcg ttgcctatac catgatgctt gcgcaggtgt tcgaccatct
     1501 gatcgcgctg gcgtcggaag gcggacgctt gcgcggcggg gatttgtgat gcggaactca
     1561 acaggttcaa ggctccgtag ttttgaaaat tatttcagtc tcaggtgtgt tcgaccggct
     1621 cgcccgcctc ttcgcacggg acgttctcct ccagttcggg cgagttccaa ttctgcaact
     1681 cgacgagcga acgatggtcc gtcatgtcgc tgcgcattgg cgtgatggag gccatgccct
     1741 gcttgatggc gtaatagtct gtgccctcga ccgtgtcttc gggaaagacc tcttcgccga
     1801 tccagtaata ggctttgccg cgcgggtcga tcttctccgt gatgatcggg cgcgcgtttt
     1861 tgagtccctg tcgcgttata cgcacgcccc gcaactcgcc gggcggcatg ttgatgttca
     1921 agagcgtccc ctgcggcaag ccttcggcta aaactttttt gacgatcttg atagcgaact
     1981 gcgcggcgta agtgaagtca taattttcgc gcgctacgag actgaaagcc agccccgtga
     2041 cgccgaggat cgtggcttcg agcgcgcccg cgaccgtgcc ggagtaggaa gcgtcgtcgc
     2101 cgaggttgcc gccgtgattg atgccggaga cgcagatgtg cggccattcc tcgcggggca
     2161 gaatcttatg cagggcgagc gttacgcagt cggtcggcgt gccgtcaaca gtccagtggc
     2221 gttcgtcaat ctggcgtatg cgcagggggc gcgagagcgt caggctgtgc gacgcgccgc
     2281 tcatctcgga ggccggagcc accgtgtaaa cgtcgcccac cgcgcgtaga gcttcttcga
     2341 gtttgatgag gccgtcggaa tgaataccgt cgtcgttagt taaaagaata cgaatcataa
     2401 aaaatcaacc acacttcatt acaacccaaa gcaacgaatt attcaagcag gcactctcga
     2461 tagaaattct ctgctcacag cccgacagcg gattgcaaac ggatagaact ctctctgcac
     2521 gtcttccgtc aatcgctcgt catcttccct ctatcgcccg ccgtcttatt tgacaggacg
     2581 gcgcgcttca tttgcatgcg cgcccgccga gcaaggaaag cgacgcatct taccggacaa
     2641 gctacgccac cggaaggagt cgcggcatag acttacggca aaagccccgc gcacgtgtaa
     2701 acgcgtcgcg gggctttcaa cacatgagct ttgaggctgc ttacgcagag atcgtcttgc
     2761 cctctttttt gctgcccaga cccttgatct ggacgggggt gttcttctcc acatcgcccg
     2821 cgccgagttt ctgcttgagc accatctcct ggtagagctt gcgcatcatc gccgcttcct
     2881 cagccgccgt cttcggtccg gccatctcag gacgattcgg acgctgcaag tcaacttcat
     2941 tcaggatgcc ggtgaaaccc gcgtcggcca actcgacgtt tttgcccttc gcaaagattt
     3001 catgtcgccc gtgctctttg taccacttgg caatcgtcgc gttctggtgc atctgctcga
     3061 tgatgttgcg ccagccgatg cccgtgttgt aagcgcagaa cgaaatctcg ccctcctgcg
     3121 tgccgtaagg gatgatgcac atctccgtgc ggcggaagtc gtagttgaag aggtcttgga
     3181 accacatgcc cgcgatgaag aggaaattcc aggggtcgct acgacgcttc tcgatgtcct
     3241 caaacgtgcg gtcgccttca accttgccgt aagacttgcc ggagagtccg aacgacttgt
     3301 cgaacttctt gaagatgtcc acgagcgtca aactctgcgg cgcgccgtaa ggctggtagt
     3361 tcttcaacag cgccaagccc atcatgatgt tcgagaacca cttgccgcgc cccgcgtccg
     3421 taatgcccgt catgtctttg acgagtcccg gaatgttcag gaactcaggc acgggcttca
     3481 tctctttcgt gttcttgttg atcatcaccg ccgtgccgac gccgcagttc gggtggcaac
     3541 cgcaggaaac ctgaccccat tcggcttcgg gaccgtgcac gaggtctgcg aagtccgcga
     3601 acgcgcccat cagcgagagc gggaaccagt cgcgcgtcgg ctctgtaatg ccgacctgct
     3661 tcttcacgtc gtgcgcgagg tgcgacagcg tgtaacgctg agccatgcga cgctcatccg
     3721 tgatctcttc gtcgcgcccg gtgaaagaga cgggctgaaa cgaaataaag gcgaccttct
     3781 tcggattgtc caaggcgaag cggatgacgg agccgacctg atcgttgttg atattgttga
     3841 cgagcgtggt gacgagcacg atctcgacgc ctgcttcatg cagattatcg atcgcgcgca
     3901 acttcacgtc gaagaggttg ccgacctgcc ggtgcgagtt cgcatcattg ccgatgccgt
     3961 cgaattgcag gtaagcatag cgcaagcccg cctctgcggc ttcgcggcag aactctttgc
     4021 tcttcgcaaa ctcaatgccg ttcgtcgcgc actgcacgga gttgtagccg actttgcgcg
     4081 agtagcgtat ggattcgatg aagtggggcg agatcgtcgg ctcgccgccc gaaaactgca
     4141 cagacatctg acggcgcggc ttgatcgaga tcgctttgtc gagaatgtct ttcacgtcat
     4201 cgaagctgag ttcgtgcacg aagccgacct gattggcgtc catgaagcac gggtcgcaca
     4261 tcatgttgca gcggttcgtc aggtcaatcg tcaagactga accgcgaccg tatttgatcg
     4321 tggaagaacc gtgattgtgc aacttctcat cgttgtgcgc ctgtatgtcg cgaccgggaa
     4381 actgcgcctc gatgtgcttg aggaaggtcg agtccatcgc catgatgtct tcgatgtggc
     4441 cgtgcgtggg gcaatctttg atcatccaaa tctcgccgtt gcgctccacg atctgcgcct
     4501 tcacctcgcc caccttctcg ttgatcagga tggccgggtc gatcacctcc gcgctcaaaa
     4561 tcttggcgcg ggcttcgatc acgcacttcg gacagaggct atcggtcgtg cgcggaaagc
     4621 cgagaggcgg cttcgatttc tgccatgatt tcaacagcgg cttgtccgac cacttcggga
     4681 tgaacgattc gctcgggcga tatttattaa aaaactgaat ggtgttgaac gcgcccgccg
     4741 caagggcggt cagtcctctt tcaacgtgtt tgatgggtcg cattttctct tccttctcta
     4801 ccggataaat
//
LOCUS       DASWNS010000488        10070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_173294, whole genome shotgun sequence.
ACCESSION   DASWNS010000488 DASWNS010000000
VERSION     DASWNS010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173294"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..416)
                     /locus_tag="VGB76_16730"
     CDS             complement(60..416)
                     /locus_tag="VGB76_16730"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0100593.1"
                     /translation="MDRRVQIIISSLQEDLRREMILDELAHSLNLSRSRLQHLFKSET
                     GLPPAQYLRRLRLERAKDLMRNSVLSVKQVMTSVGISDKSHFERAFKKAYGLTPRQYR
                     MSHLALSLGKGTGNGR"
     gene            1251..2396
                     /locus_tag="VGB76_16735"
     CDS             1251..2396
                     /locus_tag="VGB76_16735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0100594.1"
                     /translation="MTDAEPRVKAFDLRAVDEIIAGTVAPAAVEVDRDGAFPRAALDA
                     FGQAGLLGLVSSREVGGLGESHRAATQVVERVASTCASTAMVLCMHYAGSAVIEAHAP
                     REVREAVARGEHVTTLAFSEQGSRSHFWAPVGTATPDGGGVRLDAQKSWVTSAGEADS
                     YVWSSRPLEAEGQSTVWLVPAASAGISIPAPFDGLGLRGNSSSPVTAEGVRIPRGNML
                     GADGGGFDVMMGVVLPYFQLMSAGFSTGTMEAATLKAAAHVASTRLEHLGSSLADLPT
                     VRAYLSRMRIKTDMCRALLGDALTAIETGREDAMLRVLEVKAAAGESATEVTDLAMRV
                     CGGAAFRKEVGVERHFRDARAATVMAPTTDVLYDFIGKAVCGLPLFG"
     gene            2417..3280
                     /locus_tag="VGB76_16740"
     CDS             2417..3280
                     /locus_tag="VGB76_16740"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY0100595.1"
                     /translation="MSDSDTLILGAVAYDPKVVTIWEGFKVYFSRHGLDFDYVLFSNY
                     ERQVESHLAGHFHVAWNSPLAWVRTRRLAAARGIKVEAVVMRDTDRDLTSVVVVRNDS
                     GVGSVEELRGKRVAVGAIDSPQATLLPLSHLRAQRLVPGADFEVVYHDLLGGKHGDHI
                     GGEREAARALLSGDCEAACMIDGNHLLFTGEGTLPNGATRVLTQTEPFDHCNFTVAPA
                     APAGLVSRFRALLLGMSYDDPEVRPLLDLEGLKAWREGRLEGYEALERAVDEAGFYDA
                     DGNITIDGYRY"
     gene            3237..4499
                     /locus_tag="VGB76_16745"
     CDS             3237..4499
                     /locus_tag="VGB76_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100596.1"
                     /translation="MTRTATSPSTVIDIKGLGFDRGAHLLLKRALADLPTGGKLGVRG
                     SAPDLAVHLRGWCRAQGHEFVRSEAEGEGSNEAWADGDSPLVAWVARGAAFGVRWRGA
                     EAAGGADPRESGAVVERPPARWGLAARGALVEAGAPEYNFRLDSKAEVWAENVPRLYQ
                     QAAAAQWNPATAVEWDASFELPPEVESAVVQVMTYLVENETAALLVPAKFLAQVHPHF
                     REVMQLLAIQVADEARHIEVFTRRATLRRKDALGLSTAGGQQSLKTLFDEHDFTLASF
                     LLSVLGEGSFLNLLWFLERHAPDPVTRQVARLAAQDEARHVAFGMGHLQYAIALDPTL
                     KGRLASAAVRRHDALAQTAGLNEEVFDALVILAAGGWEPDAIAAGFEKVQTLKREMTA
                     DRTSRLIKLGFAPDEASALSNLHTRNFM"
     gene            complement(5940..6299)
                     /locus_tag="VGB76_16750"
     CDS             complement(5940..6299)
                     /locus_tag="VGB76_16750"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0100597.1"
                     /translation="MHQGNSKDHLILSALWLLVFSASSQIMIVSPMLPQLGRELNVPE
                     AVQGTLVSACALMVGLFALIIGPVSDKVGRRRVLLLGSGAMCLTLALHSAAYGCRVER
                     KDDRSRRSGSLLNLAAL"
     gene            complement(6442..6996)
                     /locus_tag="VGB76_16755"
     CDS             complement(6442..6996)
                     /locus_tag="VGB76_16755"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0100598.1"
                     /translation="MNTETNRPTDDADILSQPVSERDHARGAADAPVTLLMYGAYECP
                     HCVEANKIVKEIQSRLGERLRFIFRHFPRTNVHPHAEAAAEVAEAAGAQGKFWEMHDT
                     LFAHFNHLDGEHLVLYARELELDMERFEQAINHRSFAARVQEDFKSGEQSGVRATPTF
                     FINGAQHKGAWDVDALLKAVDDGD"
     gene            complement(7031..7681)
                     /locus_tag="VGB76_16760"
     CDS             complement(7031..7681)
                     /locus_tag="VGB76_16760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0100599.1"
                     /translation="MLDKTGRDALGDVLARIAAQLSTAKLIGHIKTSVGRISELVGAI
                     KEYSYMDQASVQEVDLHKGLDNTVLILRYKLRKKNIDVVRDYADALPRVTAYGSELNQ
                     VWTNLIDNAVDAMGDGGRLKIRTKLEPTGVLVEIRDNGPGITVDVQMHIFEPFYTTKP
                     VGEGTGLGLDAVSRIVRKHRGNILFQSKPGDTCFQVRLPLAQNRAPVEEPKPVTAH"
     gene            complement(7736..8548)
                     /locus_tag="VGB76_16765"
     CDS             complement(7736..8548)
                     /locus_tag="VGB76_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100600.1"
                     /translation="MQNLKLEGGEGGEDDDAAQTATPSVSPEDKVEALRRVQVFAGLP
                     EEQLRWFVENAVERWVEPGEVIFRRGHPADQMTVYLEGEAHSRQDETNLDGNVYIAQA
                     GDAQTEVSGKLPYSRMKEFPATSRAVTRARLLLFHERLFPEMLQRMPVLAERLVGLMS
                     DRVRESTKMDQQRDKLIVLGKLSAGLAHELNNPAAKRGANEMLGALEELRAADLRLCR
                     HNLTPEQRQFIAEFESEAIAHTSAATPLRALEQSDCEDELAAWLDEQGVAES"
     gene            complement(8551..8754)
                     /locus_tag="VGB76_16770"
     CDS             complement(8551..8754)
                     /locus_tag="VGB76_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100601.1"
                     /translation="MIPSPFKWQHYAPHIILLCALVLPLPNVPGVFVVGDVRKGSVKR
                     VASGVSEGSIAISFVHQYLSKVV"
     gene            complement(8903..9733)
                     /locus_tag="VGB76_16775"
     CDS             complement(8903..9733)
                     /locus_tag="VGB76_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100602.1"
                     /translation="MRASFESAPTGSGFDKLTLLGWSRGGMLAYAYANEETRLPEQFH
                     HVKALIPVDIAYKFNAEHEEQRAAACQRYHLNKQLLDAGQYHSPQGVGGQTFGLFAAS
                     DPSGQSPILGFPGLTNAQAALLVGSATHLLSAPFPPVPGYHFNAGEFDAAGLPAGLQF
                     TREAYFYDYLQTAAPFQSFTEQVEGEAIQCGDAEVPYDDRLAEITVPVYYVGAEGGFG
                     AFGADTLSRLGSAGISSHVVKLQTDGARAIDFGHADLFLADNARELVWSPIYNWVVSH
                     "
BASE COUNT         1885 a         2981 c         3150 g         2054 t
ORIGIN      
        1 aaaaggaaag ctgtttttgc tattaggaag ccgtttttgc tattagaaag ccgtttttgc
       61 tatctgccat ttcctgtccc tttgcccaag ctgagcgcga ggtgggacat tcggtactgt
      121 cgcggcgtga ggccataagc cttcttgaag gctcgctcaa agtgactttt gtcactgatg
      181 ccaacgcttg tcatgacttg cttgacactc aacacagaat tcctcatcag atcttttgcc
      241 cgctccagcc tgagcctccg caggtattgt gcgggaggca gaccggtttc ggacttgaag
      301 aggtgctgaa ggcgcgagcg agagagattg agcgagtgcg ccaactcatc aagaatcatc
      361 tctcggcgca agtcttcctg aagcgaggag ataataatct gcacacgtcg atccatatca
      421 tgaatctccg ataggagaaa tcgagcttcc gagctattgg caaaagcaga taacttctta
      481 aggaacggtc agtctctggt ggggcaaagc tccgagagga gctcatttca tcatctgaaa
      541 tcgggggtgc gaatgttcga tattaaagct gaccctgctg aggacgcaga atgtatgtta
      601 aaggccttgt gcctgtcaat cgaaattcta ttgacccaac ccatccaaca ccaggccgct
      661 cgcgctaaag agtgacaggc atatccggct cagcatcagg tatcttttct cagagggagg
      721 ctaaggagaa acactcatta cggttctgtt gcaagaatgg actctcataa ttggcagtgt
      781 gcaggtgtgc gcgtgacttt atgagacagg aggcatacgt cggaaggcta cggggtcacg
      841 tctatttaac gcgaccccgt agctttacag ccagcaagac tgaggatcat acacacgttc
      901 ggtcagagcg ttctccgtct atattgcctt gtcacttttc ggtcgtgtat gtaacatctg
      961 ggcacgcgtg taatttgacc tgacggtgct gccgttagat atgtctatga atggactggt
     1021 gacgtaatgc agaagaattg aaccagcgcc ttgcagtggg cgcaagtttt gcgacacgac
     1081 acttcccgcc cgcggattgc cgcgtagccc ctccttcaaa gcggggccaa cccggcgtcc
     1141 cgcggacaca atcaacttgc tcacgagatc ggaagcgcat gtgtgcgttt tccgcgcctc
     1201 aggtgcctga ccgcgcaccg gggattggga gctttgtgaa ggagacgagg atgactgatg
     1261 cggaaccacg cgtgaaggcg ttcgaccttc gtgccgttga cgagattatc gccgggacgg
     1321 tggcacccgc cgccgtcgag gtggacaggg acggagcctt tccccgcgcg gcactcgatg
     1381 ccttcgggca ggctggactt ctggggctgg tcagctcacg cgaggtgggc gggcttggag
     1441 agtcgcatcg ggctgcgacg caggtggtcg agcgtgttgc ctcgacctgt gcctcgaccg
     1501 cgatggtgct ctgtatgcac tacgcgggat ccgccgtcat cgaggcgcac gcgccgcgcg
     1561 aagtccgcga agcggtcgcg cgcggagaac acgtcacgac gctcgccttc tcggagcagg
     1621 ggtcgcgcag ccatttctgg gcgcccgtcg gcacggcgac gccggacggg ggcggcgtcc
     1681 gcctcgacgc gcagaagagc tgggtcacgt cagccgggga ggccgactcc tacgtctggt
     1741 cgagccgtcc gctggaggcc gagggacaga gcacggtctg gctcgtgcct gccgcgtcgg
     1801 caggaatctc catcccggcg ccgttcgacg ggctggggct gcgcgggaac agttcgtcac
     1861 ccgtgacggc ggaaggcgtc cgtatcccac gcgggaacat gctcggcgcc gacggcggcg
     1921 gcttcgacgt gatgatgggg gtcgtgctcc cctacttcca gttgatgagc gcgggcttct
     1981 ctacggggac gatggaggcg gcgacgttga aggccgccgc gcacgtcgcc agcacaaggc
     2041 tggaacacct cgggagttcg ctggccgacc tgccgaccgt ccgcgcctac ctctcgcgga
     2101 tgcgcatcaa gacggatatg tgccgcgcgc tgctcggcga cgctctgacg gccatcgaga
     2161 cggggaggga ggacgcgatg ctgcgcgtcc tggaggtcaa ggcggcggcg ggcgagtcgg
     2221 cgaccgaggt gacggatttg gcgatgcgcg tctgcggcgg ggcggcgttc cggaaagagg
     2281 tcggggtgga gcggcacttc cgcgacgccc gcgccgcgac cgtgatggcg ccgaccaccg
     2341 atgtcctcta cgacttcatc ggcaaggccg tctgcggcct gcccctcttc ggttaaagaa
     2401 aggcgcaaag acggcaatga gcgattccga cactctcatc ctgggcgcgg tcgcctacga
     2461 cccgaaggtc gtgacgatct gggaaggctt caaagtttac ttctcgcgcc acggcctcga
     2521 cttcgactac gtcctcttct ccaactacga gcggcaggtc gagtcacatc tggcggggca
     2581 cttccacgtc gcgtggaatt ctccgctcgc gtgggtgcgg acacgcaggc tcgccgccgc
     2641 gcgcgggata aaggttgaag ccgtcgtcat gcgcgacaca gaccgcgatc tcacctcggt
     2701 agtggtcgtg cggaacgact ctggcgtcgg gtcggtcgaa gaactgcgcg gcaagcgcgt
     2761 ggccgtcggc gcgatagact ctccgcaggc gacgctcctc ccgctctcac acctcagggc
     2821 gcagagactc gtccccggcg cagacttcga ggtcgtctac cacgacctgc tcggcggcaa
     2881 gcacggcgac cacatcggcg gtgagcgcga ggcggcgcgc gcactgcttt ccggcgactg
     2941 cgaagccgcc tgcatgatcg acggcaacca cctcctcttc acgggcgagg ggacgctccc
     3001 gaacggggct acgcgcgtgc tgacgcagac agagcctttc gaccactgca acttcaccgt
     3061 cgccccggca gctcccgcgg ggcttgtgtc gcgcttccgc gcgctgctac tgggcatgtc
     3121 ttatgacgac ccggaggtgc gcccgctcct cgacctcgaa ggactcaagg cgtggcgcga
     3181 gggtcggctc gaaggttacg aggcgctcga acgtgcggtg gacgaggcgg gattttatga
     3241 cgcggacggc aacatcacca tcgacggtta tcgatattaa agggctgggg ttcgaccgag
     3301 gggcgcatct cctgctcaag cgggcattgg ccgacctgcc gaccggcggg aaactcggcg
     3361 tcagggggag cgcccccgac ttggctgtcc acctgcgcgg ctggtgccgc gctcaggggc
     3421 acgagttcgt ccggtcggag gctgaaggag aggggtcgaa cgaagcgtgg gcggacgggg
     3481 actcgcctct ggtggcgtgg gtcgcacgcg gcgcggcgtt cggcgttcgt tggcgcggcg
     3541 cggaagcggc gggcggggca gacccgcgtg agtccggggc ggtggtcgaa cgccccccgg
     3601 cgcgttgggg cttggccgcg cgcggcgcgt tggtcgaggc tggcgcgccc gaatataact
     3661 tccggctcga ctcgaaggcg gaggtctggg cggagaacgt cccgcgcctc taccagcagg
     3721 cggcggccgc gcagtggaac cccgccacgg cggtcgaatg ggacgcgtcc ttcgaactgc
     3781 cgccggaggt cgagtcggca gtcgtgcagg tgatgaccta cctcgtcgag aacgagacgg
     3841 cggcgctgct cgtgcccgcg aagtttctgg cgcaggtgca cccgcacttc cgcgaggtca
     3901 tgcaactact cgccatccag gtcgccgacg aggcgaggca catcgaggtc ttcacacgcc
     3961 gcgccacgct gcggcggaag gatgcgttgg ggctttcgac cgcgggcggg cagcagtcgt
     4021 taaagacgct ctttgacgag cacgatttca cgctcgcctc cttcctgctc tcggttctcg
     4081 gcgaaggctc cttcctgaat ctgctctggt tcctcgaacg tcacgcgccc gaccccgtca
     4141 cgcggcaggt cgcgcggctc gccgcgcagg acgaggcgcg ccacgtggcc ttcggcatgg
     4201 gacatctgca atacgccatc gccctcgacc cgacattgaa aggacggctc gcgtcggcgg
     4261 ctgtcaggcg tcatgacgcc ttagcgcaga cggcggggtt gaacgaggag gtgttcgacg
     4321 ccctcgtcat tcttgcagcg ggcggatggg agccggacgc cattgcggcg ggattcgaga
     4381 aggtgcagac cctcaaacga gagatgaccg ctgaccggac gagccgcctc atcaaactgg
     4441 gattcgctcc tgacgaagcc agcgccctct ccaaccttca cacgcggaac tttatgtaag
     4501 tatcgccccg ggcggggcgc gcaaccgagg gagcagatag cttgaaggca accgttacag
     4561 cgctccttct ctatgagcgg agtctagagt gccactcggt ttcaagcgag gtcgccgatc
     4621 tcgaacacga atttgaccct cccaaacctc tccggtttcc agcctgacct tgatgtcgtg
     4681 cgcgtggtaa ggggatgacc tgaataggct gcatagagca tctcgcctgc ctgacacctt
     4741 cgctgccagg gaagcgcgat ctgtgtgtcg ccgaggaaat cctgatccgg gagcggcggg
     4801 tcggcgccct ggatgctacc tatcgtgctc ggagtgtaag ctagacgcgt tttggacatg
     4861 gcctcgtccc cggtctgcga tgcaattccc gcccctttcg gacgccacac tcacccaacc
     4921 cttcagagcg gcacagaccc tctctgcgaa atcccggagc gcatgcacga gaaatgggtt
     4981 aagagccggg tttgttcacg cctggatttg ggtctgtact tttacagcat tgcctttaga
     5041 agactccagt gttcacgggg cgactagacc acgaaggtga aggtgaatct attagacgaa
     5101 agtatcgacc cgcgcgacgg tgagtacgtt gagtgccacg agttgagccg ccccaaagaa
     5161 gtgaggggca gaaaccacta tgcgaggccg ggggtagttt gcagaaaata ttttagagag
     5221 cagaagatca caagcgactc ttcatcaggc ggccatctgg aacagcgaag cgatgaactt
     5281 cgcctgatat ggttggtgaa gtgagttgct tctcaggccg catccggggt gtcatcttcg
     5341 ctatttagtc tctctcttgc acccggttga cgagggttca catctctcag gatgctcttt
     5401 atttccgggt cgatggaagg ctgtgcctgc tccttacggg atggcgtgac aacatatagt
     5461 gtgtgacctt cgtggcgcga atcgctctcg cccccgtgcc ccgtttcgga gagtatttta
     5521 tcgacgaggg cttcacactc aggggtcggg atttgtgact tcggagttcg cttgttgttc
     5581 aggggtcgct catcaatccc attctcgatt aagcgttgga taaatgagtg gttgccggga
     5641 gtgctcttcg ggccgtagtt tccataaatg aaccagaggc gctccagacg ggtacgctgt
     5701 tcggtcgtga gtgtgacggg ggctgctttc cttcttatcg tcatgcgtga ttttactact
     5761 gcatttcgtg ttggggaaga tagaagtgcg tatcgttcat caactgctaa aaacaataac
     5821 ttttttaagc gggtagagtt gcaaatttaa gcgggtagag ttgcaaacat ttccacgcag
     5881 cagaatattt tgtgatagat gaaccacctc atactatgca gacagcggct aaaatatttt
     5941 cacaacgctg ccaaatttag caggctgccg ctgcgtctcg accgatcatc ttttcgttcg
     6001 acgcggcaac cgtacgccgc gctgtgcagg gcgagcgtca agcacatcgc accggagcct
     6061 aacagcagca cgcgccgccg ccccactttg tccgagacgg gaccgatgat gagcgcgaag
     6121 aggccgacca tcagagcgca ggcgctcacc agcgtgccct gcaccgcctc cggcacgttc
     6181 aactccctgc cgagctgcgg cagcatcggc gagacgatca tgatctggct gctggcggag
     6241 aagacgagca gccagagcgc agacaggatc aggtgatctt tggagttccc ttggtgcatt
     6301 aaatgatccg gcagagccgt tgcgttcaac gcaggcgact ccgcgacttt caggacaggc
     6361 gactccgcta ctttcaggaa aaggataatg ggtggacgtt agaaactgcc tgacctctta
     6421 atccccatcg tcaaccgcct cttaatcccc gtcgtcaacc gccttcagta aggcatctac
     6481 gtcccatgct cctttgtgtt gcgcaccgtt gatgaaaaaa gtcggcgtcg cccgcacgcc
     6541 gctctgctct ccgctcttga aatcttcctg cacccgcgcg gcgaacgagc gatgattgat
     6601 cgcctgctcg aacctctcca tatcgagttc taattctctg gcataaagca caaggtgctc
     6661 gccgtcgaga tgattgaagt gcgcgaagag cgtgtcgtgc atctcccaga acttgccctg
     6721 cgctccggca gcttccgcca cttcggcggc ggcttccgcg tgcgggtgaa cgttcgttct
     6781 cggaaaatgg cggaaaatga agcgcagtcg ctcgcccaac cggctttgga tctccttaac
     6841 gattttattt gcctccacgc agtgcgggca ttcgtaagcg ccgtacatca gcaacgtcac
     6901 cggggcgtcc gccgcgccgc gcgcgtggtc gcgttcgctc accggctggc tcaagatgtc
     6961 agcgtcgtct gtcggtctgt tagtttcggt gttcattaag atgactcctt tttccgcttg
     7021 tcttctcttg tcagtgagcc gtcacgggtt tcggctcctc gacgggtgcg cggttctgcg
     7081 ccagcggcag gcgaacttga aaacaggtgt cgcccggctt cgactgaaag agtatgttgc
     7141 cccggtgctt gcgcacgata cggctgacgg cgtcgaggcc gaggccggtt ccctcgccga
     7201 cgggcttcgt ggtgtagaac ggctcgaaga tgtgcatctg cacgtcaacg gtgatgcccg
     7261 gcccgttgtc gcggatttcc acgagcacgc ccgtcggctc aagtttcgtg cgtatcttca
     7321 atcggccacc gtcgcccatc gcgtccaccg cgttgtcgat caggttcgtc cagacctgat
     7381 tgagttcgct gccgtaggcc gtgacacgcg gcagagcgtc ggcgtaatcg cgcaccacgt
     7441 cgatgttctt cttcctcagt ttatacctga gaatgagcac ggtgttgtcc agccccttgt
     7501 ggagatcgac ctcctgcact gatgcctgat ccatgtagga gtattctttg atcgcgccga
     7561 cgagttccga gatgcgtccc acgctcgtct tgatgtggcc gatcagtttc gcggtcgaga
     7621 gttgcgcggc gatacgcgcc agaacatcgc cgagggcatc cctcccggtt ttgtccagca
     7681 cgcgctccag tgcttccgtg ttgacgcctg cctctgaaag tgtgggcgcg agtttctaac
     7741 tctccgcgac gccttgctcg tcgagccatg cggcgagttc gtcttcgcag tcgctctgct
     7801 ccaacgctcg caggggcgtg gccgcagacg tgtgcgcgat ggcttcgctt tcaaactccg
     7861 cgatgaactg ccgctgctcc ggcgtgaggt tgtggcggca cagacgcaaa tcagccgccc
     7921 gcaactcttc caacgcgccg agcatttcgt tcgccccgcg cttggccgcc gggttgttga
     7981 gttcgtgcgc gagtccggcg gagagtttgc cgagcactat cagcttgtcc cgctgctggt
     8041 ccatcttcgt agattcgcgc acgcggtcgc tcatcagacc gacgagacgc tctgcgagca
     8101 ccggcatccg ctgcaacatc tccgggaaca atcgttcgtg gaataagagc agcctcgcac
     8161 gcgtgacggc gcgactcgtg gcgggaaatt ccttcatccg cgagtaagga agttttccgc
     8221 tcacttccgt ctgcgcatcg cctgcctgcg cgatgtagac gtttccgtcc agattggttt
     8281 cgtcctgtcg agagtgggct tcgccttcca gatagacggt catttgatcc gccgggtgcc
     8341 ccctgcgaaa gatgacctcg cccggctcta cccacctctc gacggcgttc tcgacgaacc
     8401 acctcagttg ttcttcggga agccccgcga agacctgaac gcggcgcaga gcttcaacct
     8461 tgtcctccgg cgaaacggac ggcgtggcgg tctgcgccgc gtcgtcatcc tctccaccct
     8521 cgccgccctc taatttcaag ttctgcatac tcacactacc ttgctgagat attgatgaac
     8581 gaatgagatc gcgattgagc cttcactgac gcccgaagcg acgcgcttga ccgaaccttt
     8641 ccgcacgtcg ccgacgacga agacgccggg cacgttcggt agcggaagta ccagcgcaca
     8701 caacaggatg atgtggggtg cgtagtgctg ccacttgaaa ggcgagggga tcacgtctga
     8761 tgtgtagacc tgaggaaagt gacaggcaga gcgtgagggg acgttgatac gtgaaaagtt
     8821 atttttgaaa cacgaccgaa cttagagagc cgcccaccgg tctacagtga ggccggtggg
     8881 cggcaggcaa tccagtctgg tcttagtggc tgaccaccca gttatagatg gggctccaga
     8941 caagttctct cgcgttgtcg gcgaggaaga ggtcggcgtg tccgaaatct attgctcgcg
     9001 ccccgtccgt ttgaagtttg acgacatgcg aactgatgcc cgcgctccca agtctggaga
     9061 gcgtgtcggc tccgaacgcg ccgaacccgc cttctgcgcc gacgtagtag acggggactg
     9121 taatctcggc caaccggtcg tcgtaaggca cttcggcatc gccgcactgg atggcttcac
     9181 cctcaacctg ttcggtgaaa ctctggaagg gcgctgccgt ctggagatag tcgtagaagt
     9241 aggcttcgcg cgtgaactgt aacccggcag gaagccccgc agcgtcgaac tccccggcgt
     9301 tgaagtggta gccgggcacg ggcgggaagg gggcggacag gaggtgtgtc gccgagccga
     9361 cgagcagtgc cgcctgcgcg ttcgtcaatc cggggaagcc gaggatcgga gattgacccg
     9421 acgggtctga agcggcaaag aggccgaagg tctggccccc cacgccttgc ggcgaatggt
     9481 actgcccggc gtcgaggagt tgcttgttga ggtggtaacg ctggcaggcg gcggcgcgtt
     9541 gctcttcgtg ctcggcgttg aacttgtagg cgatgtccac cgggatgagc gccttgacgt
     9601 ggtggaactg ctcgggcagg cgcgtctcct cgttggcgta ggcgtaggcc agcatgccgc
     9661 cgcggctcca accgagcagt gtcagtttgt cgaacccgct gccggttggc gcagattcaa
     9721 acgacgcccg caaagatata cgcgtaagtc agaaggaaaa ggtatcaacg cgcccgctcc
     9781 gaccttcgtg aactttgccc aaatccgaca gcgcgaccac gactaatgcc ggatggaaag
     9841 cccgtcccgg ggctgcgtac atgactagta aggaggcgcg cggagtcaac gaagaagacg
     9901 agtgattctc cgctgactcc acgcgccttc cctccccgtt ggtactgctg aagtcattca
     9961 ataagggttt attcttctac ttggaggatc acgtcgaacc tgcctgatcg ctatgagaaa
    10021 taaaccggac gcgataactt tttcttgacg gttgagaata gcgtggggta
//
LOCUS       DASWNS010000489         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_44417, whole genome shotgun sequence.
ACCESSION   DASWNS010000489 DASWNS010000000
VERSION     DASWNS010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44417"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VGB76_16780"
     CDS             complement(<1..219)
                     /locus_tag="VGB76_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100603.1"
                     /translation="MKRLFYTSLSLLLLVNLAAAQDNWPQFRGANAAGVATEAAKTPA
                     SWNAAKAENILWKRAIPGLSHASPVVWGD"
     gene            complement(238..1767)
                     /locus_tag="VGB76_16785"
     CDS             complement(238..1767)
                     /locus_tag="VGB76_16785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0100604.1"
                     /translation="MNFRTTFRTLFFALSFLAVMLLSGSTSRQAAASDPGGGDWPMWG
                     GTPDRNMVSNMKGIPVSWDVAKKTNVKWMTALGSQTYGNPVVAGGQVYVGTNNEAPRD
                     PKVKGDKGILMAFRESDGEFLWQAVTDKLAAGRVNDWPYQGICSSPLVEGKILYYVTN
                     RGEVIALDTEGFRDKENDGDYKDEKLNGEKDADIIWRFDMMEEVGAFPHNMSNSSPVA
                     FGDLIYVSTSNGQDESHVNVPSPKAPAIIALNKKTGKLVWEDNSVFDKILHGQWSSPT
                     VAKLGGVDQVVFAQGDGWIRGYEALTGKKLWEFDTNPKDSVWPKTRNEVISTPVAYED
                     KIYISNGQDPEHGEGIGHLYCIDPTKRGDITKTGMVWHYDKIRRSVSTPAIRDGIIYQ
                     ADFSGFLHALDAKTGQVYWTHDVFAAVWGSPISIDGKIYLGDEDGDIVILQEGKTKKV
                     IAEINMASSVYSTPVPANGTLFIANRNQLYALSEQKAATPTAPATPATPAKTPEAPKK
                     P"
     gene            complement(1806..2840)
                     /locus_tag="VGB76_16790"
     CDS             complement(1806..2840)
                     /locus_tag="VGB76_16790"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0100605.1"
                     /translation="MNETIHDAATLAPPPTPERIFQTLTAYQQTAALKAAIELDIFTA
                     IAEGHDTAAKLAARCEAAERGVRILCDYLTVAQFLGKHADGRYSLTPEASLFLSRNSP
                     AYVGGIALFLSGEHIGDAFQRFTESVRKGGTVMAEQGSITPEHPMWEDFARGMASIMR
                     PAAIDIARVVGVKDLGACKILDVAAGHGIFGITLAEQNPQAEVYAVDWQNVLPIAQQN
                     AEAAGLGARYHLIPGSAFEVDFGAGYDLVLLTNFFHHFDPQTCETLMRKVHAALRDGG
                     RAVTLEFVPNEDRVTPPVEASFALVMLGTTPAGDAYTFPEFERMFQHAGFARSESHQT
                     MPGHIIVSYK"
     gene            complement(2843..>4236)
                     /locus_tag="VGB76_16795"
     CDS             complement(2843..>4236)
                     /locus_tag="VGB76_16795"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0100606.1"
                     /translation="LNTGAVVYKNLAIVTHSEENLESNEMGMIAALDATAKGELKKEQ
                     IKWSKVGWQGGYSSPVIDGERLYQVDNSANIAAFDLNSGRQLWLQNLGTIQKGSPVLA
                     DGKLYVGTENGKFFILRPSATGCEILDSDQLGTEQQPEAIIASPAVSNGRVFFATDSN
                     LYAIGRKSNATANHAAPVEGLPNPNRAAAWVQVVPTELIIKPGETVNFRVRLFDERGN
                     FIREERGATWSLDNLKGTLANGQLVAANDGGAQAGLVKATVGALTGAARVRIVPALPW
                     SVNFDAAELNSVPPTWVSAISKFAVREIDGNRALLKTTEGSSLLTRTRAYLGASNLSD
                     YTVEADVRGTEKRRQRGDAGVIAQRYALVLYGNSQSLHLEPWQPEVVRTVKQDYAWKP
                     DAWYRMKLSVENLPDGKVRARGKVWATGEPEPAAWMIERVDPLPNRQGAPGIFGNALA
                     EVYFDNIKVTANK"
BASE COUNT          698 a         1279 c         1364 g          895 t
ORIGIN      
        1 gtcgccccac acaaccgggc tggcgtgcga cagaccgggg atggctctct tccagagaat
       61 gttctcggct ttcgccgcgt tccaactcgc gggcgtcttc gccgcctccg tcgccacgcc
      121 cgccgcgttc gcgccgcgaa actgcggcca gttatcctgc gccgccgcca aattcactaa
      181 aagcaggaga gagagagaag tgtaaaagag acgcttcata gcttctttac gtttgcctta
      241 aggcttcttc ggtgcttcgg gtgtctttgc cggagttgcc ggagttgccg gagccgtggg
      301 agttgccgcc ttctgctcgg agagggcgta gagttgattg cggttggcga tgaagagcgt
      361 gccgttggcg ggcacgggcg tcgaatagac gctgcttgcc atgttgatct cggcgatgac
      421 cttcttcgtc ttgccttcct gcaagatgac gatgtcgccg tcttcgtcgc cgagataaat
      481 cttgccgtca atgctgatgg gcgaacccca gacggcggcg aacacgtcgt gcgtccagta
      541 gacctgcccg gtcttggcgt cgagcgcgtg gagaaagccg ctgaagtcgg cttgataaat
      601 gatgccgtcg cggatggcgg gcgtggagac ggagcggcgt atcttgtcgt agtgccagac
      661 catgcccgtc ttcgtgatgt cgccgcgctt cgtggggtca atgcaataga gatgaccgat
      721 gccttcgccg tgttcggggt cttggccgtt cgagatgtag attttgtctt cgtaggcgac
      781 gggcgtggag atcacttcgt tgcgcgtctt cggccagacg gaatccttcg ggttcgtgtc
      841 gaactcccag agcttcttgc ccgtcaacgc ttcgtagccg cgaatccagc cgtcgccctg
      901 cgcgaagacg acctgatcca cgccgccgag tttcgccacc gtcggcgaag accattggcc
      961 gtgcagaatc ttgtcgaaga cggagttgtc ttcccagacg agtttgcccg tcttcttgtt
     1021 gagggcgatg atggccgggg ctttcggcga agggacgttg acgtggcttt cgtcctgccc
     1081 gttggaagtg ctgacgtaga tgagatcgcc gaaggcgacg ggcgaagagt tagacatgtt
     1141 gtgcggaaac gcgccgacct cttccatcat gtcgaaacgc cagatgatgt cggcgtcctt
     1201 ctcgccgttc aacttctcat ccttgtagtc gccgtcgttc tctttgtcgc ggaagccttc
     1261 cgtgtcgagc gcgatcacct cgccgcgatt cgtgacgtaa taaagaatct tgccttcgac
     1321 taagggcgac gagcagatgc cctgatacgg ccaatcgttg acgcgacccg ccgcgagttt
     1381 gtccgtcacg gcctgccaca aaaattcgcc gtccgattcg cggaaggcca tcaggatgcc
     1441 cttgtcgcct ttcactttcg ggtcgcgcgg cgcttcgttg ttcgtgccga cgtaaacctg
     1501 cccgcccgcc acgaccgggt tgccgtaagt ttgcgagccg agcgcggtca tccacttgac
     1561 gttcgttttc ttcgccacgt cccaactcac ggggatgccc ttcatgtttg aaaccatgtt
     1621 gcggtcgggc gtgccgcccc acatcggcca atcgccgccg ccggggtcgg aagccgcggc
     1681 ctgccgcgag gtggagccgg agagaagcat cacggcgaga aacgagagcg cgaaaaacag
     1741 tgtgcggaaa gtggtgcgaa agttcatatc ggttccttga agattgatcc tgagcaatag
     1801 attctctact tgtatgaaac gatgatgtgg ccgggcatcg tctggtggga ttcgctccgc
     1861 gcgaagcccg catgctgaaa catgcgttca aactccggga aagtataagc gtcgccagcc
     1921 ggagtcgtgc cgagcatgac gagggcgaaa gaggcttcga cgggcggcgt cacgcggtct
     1981 tcgttcggca cgaattcgag cgtcacggcg cgccctccgt ctctgagcgc ggcgtgaact
     2041 ttgcgcatca gcgtctcgca ggtctgcggg tcgaagtggt gaaagaagtt cgtcaggagg
     2101 acgaggtcgt agcccgcgcc gaagtcaact tcaaacgcgc tgccgggaat cagatgataa
     2161 cgcgcgccca agcccgccgc ttccgcgttc tgttgcgcga tgggtagcac gttctgccag
     2221 tcaaccgcat agacttccgc ctgcgggttc tgctccgcga gcgtgatgcc gaagatgccg
     2281 tgccctgcgg ctacgtcgag gattttgcac gcgcccaaat ccttcacgcc gacgacgcgt
     2341 gcgatgtcta tggcggcggg gcgcatgatc gatgccatgc cgcgcgcgaa gtcttcccac
     2401 atcgggtgct ccggcgtgat cgaaccctgc tccgccatca ccgtgccgcc tttgcgcacg
     2461 ctctccgtga accgctggaa ggcgtcgccg atgtgctcgc cggagaggaa cagtgcgatg
     2521 ccgccgacgt aggcgggcga gttgcggctg aggaaaagcg aggcttcggg cgtcaggctg
     2581 taacgcccgt ccgcgtgctt gccgagaaac tgcgcaacgg tcaggtagtc gcaaaggatg
     2641 cgcacgccgc gttcggcggc ctcgcaacgc gcggcgagtt tcgcggcggt gtcgtggcct
     2701 tcggcgatgg ccgtgaagat gtccagttcg atagcggctt tcagggctgc ggtctgttga
     2761 taggccgtga gcgtctgaaa gattcgttcg ggcgtgggcg gcggggcgag cgtggccgcg
     2821 tcatggatcg tctcgttcat tgttatttgt tcgccgtgac tttgatgttg tcgaaataga
     2881 cttcggccag agcgttgccg aagatgccgg gcgcgccctg ccggttcggg agcggatcga
     2941 cgcgctcgat catccatgca gcgggttccg gctcgcccgt cgcccacact ttgccgcgcg
     3001 cgcgcacttt cccgtcgggc agattctcga ccgacaactt catgcgatac caggcatcgg
     3061 gcttccatgc gtagtcctgc ttgacggtgc gcaccacttc cggctgccac ggttcgagat
     3121 gaagcgactg gctgttgccg tagagcacga gcgcgtaacg ctgcgcgatc actccggcgt
     3181 cgccgcgctg gcggcgtttc tccgtgccgc gcacgtcggc ctcgaccgtg tagtcggaca
     3241 aattcgacgc ccccagataa gcgcgcgtgc gcgtcaaaag cgaagagcct tccgtcgtct
     3301 tgaggagcgc gcggtttccg tctatctcgc gcacggcgaa tttcgagatc gcgctcaccc
     3361 acgtcggcgg cacgctgttc aattccgccg cgtcgaagtt cacgctccac ggcagcgcgg
     3421 ggacgatgcg cacgcgcgcc gcgcccgtca acgcgccgac cgtcgctttg acgagtccgg
     3481 cctgcgcgcc gccgtcgttc gccgcgacga gttgaccgtt cgccagcgtt cccttcaagt
     3541 tatcgagcga ccacgttgcg ccccgttcct cgcggatgaa gttgccgcgt tcgtcgaaca
     3601 agcgcacgcg gaagttgacc gtctcgcccg gtttgatgat caactcggtg gggacgacct
     3661 gcacccacgc ggcggcgcgg ttcgggttgg gcagcccttc gaccggggcg gcatggttgg
     3721 ccgtcgcgtt ggacttcctg ccgatggcgt agaggttcga gtcggtggcg aagaagacgc
     3781 gcccgttcga gacggcgggc gaagcgatga tcgcctccgg ttgctgctcc gtgccgagtt
     3841 gatccgagtc gagaatttcg cagcccgtgg cagacgggcg gaggatgaag aattttccgt
     3901 tctccgtgcc gacgtagagt ttgccgtcgg cgagcacggg cgaccccttc tggatcgtgc
     3961 cgaggttttg cagccagagt tgcctgccgc tgttcaggtc gaaggcggcg atgttcgcgc
     4021 tgttgtcaac ctgataaagc cgctcgccgt caatgacggg cgaagagtag ccgccctgcc
     4081 agccgacttt gctccacttg atctgttcct tcttgagttc gcccttcgcc gtcgcgtcga
     4141 gcgcggcgat catgcccatc tcgttcgatt cgagattctc ttcgctgtgc gtcacgatgg
     4201 cgagattttt gtagacgacc gcgcccgtgt tgaggc
//
LOCUS       DASWNS010000490         3759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_123083, whole genome shotgun sequence.
ACCESSION   DASWNS010000490 DASWNS010000000
VERSION     DASWNS010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123083"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VGB76_16800"
     CDS             complement(<1..923)
                     /locus_tag="VGB76_16800"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEY0100607.1"
                     /translation="MWLDSVSIYRLGLLLLLLITSTQSARAQAIFEMPEGVESRWASG
                     ENPKGEKGKGGQANAGRKGAPTVAIKAGESRVLAEAVGTSGTVRRIWMTFPDRSPRML
                     RSLRIDMYWDNARTPAVSAPLGDFFGIGLGQTTVFQSALFSNPEGRSFNCFIPMPFKT
                     GMKIVMTNESSDNLEELFYDVDYTLGDKHNAQMLYFHAHYRRENPTRLQQDFELLPLV
                     SGRGRYLGTNIGVIVNRKTYYNTWWGEGEVKIYLDGDREFPTLAGTGTEDYVGTAWGQ
                     GAFAHDYQGSPIADEKNLRWCFYRYHIPDPI"
     gene            1290..1976
                     /locus_tag="VGB76_16805"
     CDS             1290..1976
                     /locus_tag="VGB76_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100608.1"
                     /translation="MMHIVNVKYLLAVFMLAFLVAPLNQTASLDDWKESLAASKSGKG
                     CESIPYSTLRSQCISNSARVGASCKTEAWSCDELGTKGLKATMTALSGHIERLKEQKK
                     QLESQKTRATSATEKTDLGKKIADVDKQISEKSRTFDGFETKLASDLVKIKDRLAKGS
                     QCLDARNDVQSVFKSAGDAARRESARDVKVIADSLTDYWEKKRDEHQKAFENVKLGID
                     KCNKSRDGLL"
     gene            complement(2015..3430)
                     /locus_tag="VGB76_16810"
     CDS             complement(2015..3430)
                     /locus_tag="VGB76_16810"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0100609.1"
                     /translation="MAKGRVLVIDDEPLVLDTLREVLTAGGFEAAAEGDARRALERVA
                     SFHPSVVISDVKMPNLNGFELLRELRTRHPEIAVILLTGEGSVEMALRAIQDEGAYHY
                     FEKPIDNEKLCAVIERAIAEGKAARDGVALRHELKGSGAFGKLIGNSEPMQRVYKLVE
                     QVSPSSASVLITGESGTGKELVARTIHELSPRRAAEFVAINCSAIPETLMESELFGHE
                     RGAFTGASSRRQGCFELAHRGTLLLDEIAEMPALLQAKLLRVLEERSVRRLGGSQEIP
                     IDVRVLAATNREPHEAVRTGAFREDLLYRLNVITIELPPLRQRKEDLPLLVQHLITQL
                     SERHERPARHLSAAALEVLRSHHWPGNVRELRNVIERAVIICSGETIERHHIAPYPLD
                     QRARARVEDTITLPVGTPIEEVERQMILRTLQKTDNNKTRAAELLQISLKTLHNKLRL
                     YRERNQLTDLDPARPNPTPSV"
BASE COUNT          782 a         1032 c         1142 g          803 t
ORIGIN      
        1 tagatggggt cggggatgtg gtagcggtag aaacaccagc gcaggttctt ctcgtcggcg
       61 atagggctgc cttgataatc gtgcgcgaac gcgccctgcc cccacgccgt gccgacgtaa
      121 tcttccgttc ccgttccggc gagcgtggga aattcacgat ccccgtcgag atagatttta
      181 acttcgccct cgccccacca tgtattgtag taggtcttcc ggttgacgat gacgccgatg
      241 ttcgtgccga ggtagcggcc acgaccggag actagcggga gcaactcgaa gtcctgctgg
      301 agccgcgtcg ggttttcgcg gcgatagtgc gcgtggaagt agagcatctg cgcgttgtgc
      361 ttgtcgccga gcgtgtagtc cacgtcgtag aaaagctctt cgagattgtc gctgctctcg
      421 ttcgtcatca cgatcttcat gccggtcttg aaaggcatcg ggatgaagca gttgaaactg
      481 cgcccttccg ggttggagaa gagcgccgac tgaaagacgg tcgtttgtcc gagcccgatg
      541 ccgaagaagt ctccgagcgg cgcgctcacg gcgggagtcc tggcattgtc ccagtacatg
      601 tctatgcgca aactgcgcag catgcgcggg ctgcggtcgg gaaacgtcat ccagattcta
      661 cgcaccgtgc cgctcgtgcc cacggcttcg gccagcacac gactctcgcc cgccttgatc
      721 gccaccgtcg gcgcaccttt gcgcccggcg ttcgcctgcc cgcctttgcc tttttcgccc
      781 ttcggattct ctccgctcgc ccagcgcgac tcgactccct ccggcatctc gaagatcgcc
      841 tgcgcacgcg ccgactgagt tgaagttatt agaagcaaca acaacaatcc gagacgataa
      901 atagaaacgc tgtcaagcca catattttat gctcctctct attcgattct ttccctgacg
      961 tttgaattaa ccccgtgagc aatttgataa gggttaatgc tgagacgagt cgaaagcagt
     1021 agcggatagc aggcaagccg cgccggctgt ctccaaaacc gctcaaaccc gaccatattg
     1081 tgtaatgcgt agaagagaca ctataatcaa aatctcaggg taatgttaga cttttccctc
     1141 atagtagaaa ttagactcat tgctctaccc gctgttgtaa atgtcgtctc tcggctaatt
     1201 gccaaatcca cctcggcgtt tcgtaatccc actaactatg ttccgcttcg gcagtttcac
     1261 agaagcgcaa ttgaagagga gataagcaga tgatgcacat cgtaaacgtt aaatacctgc
     1321 tggccgtttt catgctggcc ttccttgtcg cgcctttgaa tcaaacagcg agcctcgacg
     1381 attggaagga atcgctggcg gcctcgaaga gcggcaaggg atgcgaatct attccatatt
     1441 ccactcttcg ctcccagtgc attagcaatt cggcgagagt cggagcgtcg tgcaagacgg
     1501 aagcgtggag ttgcgatgaa cttggaacta agggtcttaa ggctaccatg acggcactat
     1561 ccggtcacat cgagcggctc aaggagcaga aaaagcaact ggaaagccag aagacgcgtg
     1621 cgacaagcgc gacggaaaag accgacctcg gcaaaaagat agctgacgtg gacaagcaaa
     1681 tttcagaaaa atcgagaacc ttcgacggct ttgagacgaa acttgcgagc gacctcgtta
     1741 agattaagga tagacttgcc aaaggcagcc agtgcctcga tgctcgaaac gatgtccaga
     1801 gtgtatttaa gagtgcagga gatgcggcac gacgcgagag cgcgcgtgat gttaaggtca
     1861 tcgctgactc gttgacagac tactgggaga aaaaaaggga cgagcatcag aaggcgttcg
     1921 agaatgtgaa gctcggcatt gataaatgta acaagagcag ggatggactt ttgtgatgcc
     1981 cggcgggatt ctattttgct ctactttgta acattcacac gctaggggtc gggttggggc
     2041 gagcggggtc gaggtctgtg agttggttgc gttcgcggta gaggcggagt ttgttgtgga
     2101 gggtcttgag gctgatctgt agaagttcgg cggcgcgggt tttgttgttg tcggtctttt
     2161 gcagcgtgcg caggatcatc tggcgttcga cctcttcgat gggcgtgccg acgggcagcg
     2221 tgatggtgtc ttcgacgcgt gcacgggcgc gctggtcgag cgggtagggc gcaatgtgat
     2281 gccgctcgat ggtttcgccg gagcagatga tgacggcgcg ctcgatgacg ttgcggagtt
     2341 cgcgcacgtt gcccggccag tggtgcgagc ggagcacttc gagcgcggct gcgctcaggt
     2401 ggcgcgcggg tcgctcgtgc cgttcggaga gttgcgtgat gagatgctgc acgagcagcg
     2461 gcaagtcttc cttgcgctgg cgtagcggcg ggagttcgat ggtgatgacg ttcagacgat
     2521 agagcaaatc ttcgcgaaac gctcccgtgc gcacggcttc gtgcggctcg cggttggtcg
     2581 ccgccagcac gcgcacgtca atcggaatct cctgcgagcc gccgagccgc cgcaccgaac
     2641 gctcttccag aacgcgcagc agcttggctt gcaggagcgc gggcatctcc gcgatctcgt
     2701 ctaagagaag cgtgccgcga tgcgcgagtt cgaaacaacc ctgacggcgc gaacttgcgc
     2761 ccgtgaatgc gccgcgctcg tggccgaaca gttccgactc catcaacgtc tcagggatcg
     2821 ccgaacagtt gatcgccaca aactcggctg cgcggcgcgg gctgagttcg tggatggtgc
     2881 gcgcgacgag ttctttgccc gtgccggact cgcccgtgat caagaccgaa gcggaagagg
     2941 gcgagacttg ttcgacgagt ttatacacgc gctgcatcgg ctcggagttg ccgatcaact
     3001 tcccgaacgc gccgctcccc ttgagttcgt gccggagggc gacgccgtcg cgcgcggcct
     3061 tgccctcggc aatcgcgcgc tcgatgacgg cgcaaagttt ctcgttatca atcggcttct
     3121 cgaagtagtg gtaggcgccc tcgtcctgta tcgcgcgcag agccatctcg accgaaccct
     3181 cgccggtcaa aagtatgacc gcgatctccg ggtggcgcgt gcgcaactcg cggagcaact
     3241 cgaacccgtt gagattcggc atcttgacat cggagatgac gaccgacgga tgaaacgaag
     3301 cgacgcgttc gagggcgcgg cgtgcatcgc cttcagccgc cgcttcgaac ccgcccgccg
     3361 tcaacacctc acgcaaggta tccaacacga gcggctcatc atcaatcact aagacgcgtc
     3421 ccttcgccat aaacgcagtt tagcatacgg cgtttgggga agaagcaggg aaacgataaa
     3481 ggagaatcca gaagtcagaa gtcagaagtc aggagtcaga agaaaacctg cgtgagtctt
     3541 aacttctgac tcctgtattc tgttttctgt cttctcctcc atccttcatc gttcattttt
     3601 accgtgtgaa gtgttcggcg atagcgcgtg cgagtgcgcc ggtcgtggac tctgcgggct
     3661 ggatgtcggt cgagagattg tagtcggcgg cggtgcgggc ggtgatgtcg ccgatgcagg
     3721 cgacgcttac gtctttcagg aggccgctca ggtcgcgcg
//
LOCUS       DASWNS010000491         4246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_44511, whole genome shotgun sequence.
ACCESSION   DASWNS010000491 DASWNS010000000
VERSION     DASWNS010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44511"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..607)
                     /locus_tag="VGB76_16815"
     CDS             complement(101..607)
                     /locus_tag="VGB76_16815"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEY0100610.1"
                     /translation="MAVEIRAVVITLSDACARGERVDESGAALVELLRGMQAEIVATE
                     VLPDDLEPLADKLRDYAGRDEVNLILTTGGTGFSPRDNTPEATRAVIERDAPGLAEAM
                     RAETLRQTPTAMISRGVCGIRAGTLIINLPGSPKAVRECFAVVRPVLGHAVTLLAGHS
                     HEEAKQAT"
     gene            complement(672..1088)
                     /locus_tag="VGB76_16820"
     CDS             complement(672..1088)
                     /locus_tag="VGB76_16820"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY0100611.1"
                     /translation="MEINSSEAIKRILDECKTITVVGLSSNTWRASHSVSAYMQAKGY
                     RIIPVNPNETQVLDEQVYASLADVPEKPELVNVFRRSEEAGAVVDEAIRLGAKAVWLQ
                     EGVIDRAAARRALDAGLLVVMDRCILKEHARLSRSM"
     gene            complement(1178..>4246)
                     /gene="carB"
                     /locus_tag="VGB76_16825"
     CDS             complement(1178..>4246)
                     /gene="carB"
                     /locus_tag="VGB76_16825"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY0100612.1"
                     /translation="PATIMTDPDTADHTYVEPLTLASVTEIIRKERPDALLPTVGGQT
                     ALNLAVGLADAGVLDEFGVEMIGAKREAVKIAEDRQLFKAAMDEAGLPMPRGGFARTW
                     GEAEQLVETTGYPAIIRPSFTLGGWGGGVAFNPEEYEEIVRHGLAASPISEVLIEESI
                     LGWKEYELEVMRDLHDNVVIICSIENFDPMGVHTGDSITVAPAQTLTDVEYQALRDMS
                     ITVIRKVGVETGGSNIQFAVNPENGDVRVIEMNPRVSRSSALASKATGFPIAKIAAKL
                     AVGYTLDEIPNDITKKTPASFEPTIDYVVAKIPKWNFEKFREAEDVLGTQMKSVGEVM
                     AIGRTFKEALFKGLRSLEATKPLRLADVPADELQRKLARPNSQRFSYITYALQHGVSI
                     EEIHRLSRIDPWFLEQLQDVMLIQERIEGRTLEEISDAALREAKEVGLSDRRIAYLTK
                     RTEDEVRAHRKRLGIVPVYKRVDTCGAEFESFTPYLYSTYEEEDESAPTDRRKIMILG
                     SGPNRIGQGIEFDYCCCHASFALSDAGFETIMVNCNPETVSTDYDTSDRLYFEPLTFE
                     DVMNIIDVEQPEGVIVQFGGQTPLNLAMRLDAAGAPIIGTSPDSIDLAEDRKRFGALL
                     RTLGIPQPENGTATSPAEAIIEAERIGYPVLVRPSYVLGGRAMAIVYDEKSLDEYMRT
                     AVDASPQKPILIDKFLERAAEFDVDALADADTCVVAGIQEHIEEAGIHSGDSSCVLPP
                     VRIAPEHVETMRHYTRMLAEALKVRGLLNLQFAIKDDRVYVLEVNPRASRTVPFVSKA
                     TGVPLARIAALVMAGRPLASFNLPPDLSVHRFFIKSPVFPFVKFAGVDPILGPEMHST
                     GEVMGIGDTFGEAYAKAMMGAGLELPSSPGTAFISVNDADKGQAVVVARRLGRLGFNL
                     MATFGTAARLSEVGLEVESVFKVNEGRPNIVDHIKQGEIALVINTPLGRASHFDEQAI
                     RRAALQHNVPCVTTMTGAQALVEAITAFGDGKTTKPISLQELHAVASAAK"
BASE COUNT          763 a         1360 c         1262 g          861 t
ORIGIN      
        1 cggagatcat cacacatgat ctccgcccct tctactttaa ccgcgcgtcg tcatcaagcg
       61 aattcgttct cttccctttg ctcgcctgtg cgctcgtaat tcacgtcgcc tgttttgctt
      121 cttcgtgcga atgtccggcc agaagcgtga cggcatggcc gagcaccgga cgcaccacgg
      181 cgaaacattc gcgcacggct ttgggcgagc cggggagatt gataatgagc gtgcccgcgc
      241 ggatgccgca gacgccacgc gagatcatcg cggtcggcgt ctgtctcaaa gtctccgcac
      301 gcatggcttc ggcgagtccc ggcgcgtcgc gttcgatgac ggcgcgcgtc gcctccggcg
      361 tattgtcgcg tgggctgaag cccgtcccgc ccgtcgtcaa aatgagattc acttcgtcgc
      421 gtccggcgta gtcgcgcagt ttatccgcga gcggttcgag gtcgtcaggc aacacttccg
      481 tcgcgacaat ctcagcctgc atgcctcgca gcagttccac caacgccgcg cccgactcgt
      541 ccacacgctc cccacgcgcg cacgcgtcgc tcaaggtgat gaccaccgct cgaatctcaa
      601 ctgccatgaa tttgatattg agagttgctt aaaaagagta ctggcaggtt cgctcaactg
      661 ccctttgtcg tctacatcga acgcgagagg cgcgcgtgct ctttcaggat gcagcgatcc
      721 ataacgacga ggagcccggc gtcaagcgcg cgtctcgctg ccgccctgtc aatcacaccc
      781 tcttgcagcc agaccgcttt tgcgccgagg cggatcgctt cgtcaaccac cgcgcccgcc
      841 tcttcggaac gacgaaacac gttgacgagt tcgggcttct ccggcacgtc cgcgagcgac
      901 gcgtaaacct gctcgtcgag cacctgcgtc tcgttcggat tgacggggat gatgcgatac
      961 cccttggcct gcatgtaagc cgacacgcta tgcgacgcgc gccacgtgtt cgaagacaac
     1021 ccgaccaccg tgatcgtctt acattcatca agaatccgct tgatggcctc cgaggaattg
     1081 atctccacta ttcacctccg gttctatgtc tcaagtctcc acgctcaagt cccggatcaa
     1141 agagatcgtt ttcagactca ggactcggag cgcggaatca tttcgctgcc gacgcgaccg
     1201 cgtgtagttc ctgcaacgag atcggctttg tggtcttgcc gtcgccaaac gcggtgatgg
     1261 cttcgacgag agcctgcgcg ccggtcatcg tcgtaacgca cggcacgttg tgctggagcg
     1321 cggcgcggcg gatggcctgc tcgtcgaagt gagatgcgcg ccccaaaggc gtgttgatga
     1381 cgagtgcgat ctcgccctgc ttgatgtgat ccacgatgtt cgggcggcct tcgttgacct
     1441 tgaagaccga ctccacctcc aagcccactt cgctcagacg cgccgccgtg ccgaacgtcg
     1501 ccatgagatt gaaaccgagc cgccccagcc gacgcgcgac aaccactgcc tgtcccttgt
     1561 ccgcatcgtt gacgctgata aaagccgtgc ccggactcga cgggagttcc agccccgcgc
     1621 ccatcatcgc cttcgcgtaa gcctctccga aggtgtcgcc gatgcccatc acctcgcccg
     1681 tcgaatgcat ctcaggaccg aggatcggat cgacgcccgc aaacttgacg aacgggaaga
     1741 ccggcgactt gatgaagaaa cgatgcacgc tcaaatccgg cggcaggttg aaggaggcga
     1801 gcggacgccc cgccatcacg agcgcggcga tgcgcgccag cggtacgccc gtcgctttcg
     1861 atacgaaggg caccgtgcgc gatgcgcgcg ggttcacttc gagcacatag acgcggtcgt
     1921 ccttgatcgc aaactggagg ttgagcaggc cgcgcacttt cagagcctcc gccagcatcc
     1981 gcgtgtaatg tcgcatcgtc tcgacgtgtt cgggcgcgat gcgcacgggc ggcaggacgc
     2041 acgaagagtc gcccgaatga attcccgcct cctcgatgtg ctcctgaatt cccgcgacga
     2101 cgcaggtgtc cgcatccgcc agcgcgtcca cgtcgaactc cgccgcgcgt tcgagaaact
     2161 tatcgatcag gatcggtttt tgcggcgacg catcaaccgc cgtcctcatg tattcgtcca
     2221 gactcttctc gtcgtagaca atcgccatcg cccgcccgcc gagcacgtac gacgggcgca
     2281 cgagcaccgg gtagccgatg cgttccgcct ctatgatcgc ttccgcaggc gacgtggccg
     2341 tgccgttctc cggctgcggg atgccgagcg tgcgcaggag cgcgccgaag cgtttgcgat
     2401 cttccgcgag atcaatcgaa tcgggcgagg tgccgatgat gggtgcgccc gccgcatcga
     2461 gtcgcatcgc gagattgagc ggcgtctgcc cgccgaactg cacgatgacg ccttcgggtt
     2521 gctccacgtc gatgatgttc atcacatctt caaacgtgag cggctcgaag tagagacggt
     2581 cggaagtgtc gtagtcggtc gagacggttt cgggattgca gttcaccatg atcgtctcga
     2641 agcccgcgtc gctcaaggcg aacgacgcat gacagcagca gtaatcaaac tcgattccct
     2701 gcccgatgcg gttcggaccg gagccgagga tcatgatctt gcgcctgtct gtaggcgcgg
     2761 attcgtcttc ctcttcgtaa gtcgaataga gatagggcgt gaacgattca aactccgcgc
     2821 cgcaggtgtc cacgcgcttg tacacgggca cgatgccgag ccgcttgcgg tgcgcgcgca
     2881 cctcgtcctc cgtgcgcttg gtgagatagg cgatgcggcg atccgacagg ccgacctctt
     2941 tcgcttcgcg cagggctgcg tcggaaatct cttcgagcgt gcgcccttcg atccgctctt
     3001 gaataagcat cacgtcttgc agttgttcga gaaaccaggg gtcgatgcgc gagaggcgat
     3061 gaatctcttc gatggagacg ccgtgctgca aagcgtaagt gatgtaagag aagcgttggg
     3121 aattggggcg cgcgagcttg cgttgcaact cgtctgcggg cacgtccgcg agacgcaggg
     3181 gcttcgtcgc ttcgagcgag cgcaagcctt tgaacagagc ctctttgaac gtgcgtccaa
     3241 tcgccatcac ttcgccgacc gacttcatct gcgtgccgag tacgtcttcg gcttcgcgaa
     3301 acttctcgaa gttccacttc ggaatcttcg ccaccacgta gtcaatcgtc ggttcgaaag
     3361 aggcgggcgt cttcttggtg atgtcgttcg gtatctcgtc gagcgtgtac ccgacggcga
     3421 gtttcgccgc gatcttggcg atgggaaagc ccgtggcctt cgaggcgagc gcggaggaac
     3481 gcgagacgcg cgggttcatc tcgatgacgc gcacgtcgcc gttttccggg ttgacggcga
     3541 actgtatatt cgagccgccc gtctcgacgc cgactttgcg aatcaccgtg atggacatat
     3601 cgcgcaaggc ttgatattcc acgtcggtta aagtctgcgc gggcgcgacc gtgatcgagt
     3661 cgcccgtgtg cacgcccatc ggatcgaagt tttcaatcga gcagatgatc accacgttgt
     3721 catgcaggtc gcgcataact tcgagttcat actctttcca accgagaatt gattcttcga
     3781 tcaacacttc gctgatcggc gacgcggcga gtccgtgcct gacgatctct tcgtactctt
     3841 ccgggttgaa agccacgccg ccgccccagc cgccgagcgt gaaactcgga cgaatgatcg
     3901 ccggatagcc ggtcgtctcc acgagttgtt cggcctcgcc ccaggtgcgc gcgaagccgc
     3961 cgcgcggcat cggcaagccc gcttcgtcca tcgccgcttt gaacagttgg cggtcttccg
     4021 cgatcttcac ggcctcgcgc ttcgccccga tcatctcgac cccgaattcg tccagcacgc
     4081 ccgcatccgc cagcccgacg gcgaggttca gggcggtctg cccgccgacg gtcggcagca
     4141 gcgcgtccgg tcgctctttg cgaatgatct ctgtgacgga ggcgagggtg agcggctcga
     4201 cgtacgtgtg atctgccgtg tcggggtcgg tcatgatcgt cgccgg
//
LOCUS       DASWNS010000492         9970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_201051, whole genome shotgun sequence.
ACCESSION   DASWNS010000492 DASWNS010000000
VERSION     DASWNS010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201051"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGB76_16830"
     CDS             <1..444
                     /locus_tag="VGB76_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100613.1"
                     /translation="QILERLLQSVDNTVGTVGGVANTALQPGGVVDRTVGTVGQTVNT
                     LAQPGGLLSSTVNTLGQTLQRTVDRTGNIVERTLDTAGTVVNQRTVGNVSSLSVLRET
                     TNAAGQVLRQVRDTSGAVIELTLDAAGRITNSRVVSQATGTNPRQ"
     gene            704..1369
                     /locus_tag="VGB76_16835"
     CDS             704..1369
                     /locus_tag="VGB76_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100614.1"
                     /translation="MIENLNLASNPFRNRTLPWTVAAIIACVSVVALIFTISSFRQTR
                     DRASSIERDVRGLRTEEASLRAKADAVTQALTPDQLRTLEAAHTLIDQRNFSWSRLFA
                     DLEAVLPQGVRVARIGVRDVAHSGGAELDLAIVARTPADITGMLGTMAQGRTFSADLL
                     TENKSDKGIESTLRVRYTPGASRPATPDVVESASGSSSGASENMIAGASNNSVASAPG
                     ETR"
     gene            1366..2160
                     /locus_tag="VGB76_16840"
     CDS             1366..2160
                     /locus_tag="VGB76_16840"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HEY0100615.1"
                     /translation="MKETRPDAGAEDKRRTISPAQIRARLAGRRTTRPTGAIRLPEII
                     ALSVAILLLLGAACAYFFLLVPERARLVALENERAQLQAKLRASTEGVERNESTQASV
                     GKIVSSLNEFETSVLVAREPQTSTAVIAELNDKTRRHNLSRAQFSFSHLDEFVPGQTN
                     RQQSNRRSGAGAGTESKRESVFPTIDISLTIEGPYGNLRRFIRDIEQSRRFIVINGIQ
                     LEGVSDTGSAARAALVSLRLDMSAHFRRSGAAVASGATSVPAATSQ"
     gene            2183..3142
                     /locus_tag="VGB76_16845"
     CDS             2183..3142
                     /locus_tag="VGB76_16845"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY0100616.1"
                     /translation="MALFEGKTPAERNKMIAAIAFGALALLLLGRMFFGSDDKPPPRA
                     AGKAKARPTSGTTPGGDGANGAQPAPADELIQMPRELNIERVSYNVPDAGRNIFAFYV
                     PPVKPTPNPAEVAATPPPTPEPTPPLMLSAISPSSVMARTGDFALQITGDKFTPQSRV
                     YVDGQELPTTFASAQQLSTNVPGVLINAPGSRTIVVRTPDNSLYSNQTMLNVAAPPTP
                     QYTYVGILGSPRYSDKAILKAQNNNELITVQRGDTVAGRFKVTSISTRAVEFTDAQLQ
                     IKHTLPYVENRATGGGSPGSPSPRFNPPPKPADDDGDDDEPQD"
     gene            3126..3836
                     /locus_tag="VGB76_16850"
     CDS             3126..3836
                     /locus_tag="VGB76_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100617.1"
                     /translation="MNLKIKDQRQTPPPAVAVATQPGTGARAGERGYTLVALLALMTI
                     LALALTAAAPRLRQQAQRNLEREAIARGEEVAEAIALFQLAKGTPPKSMDDLLEGVEP
                     FAGAFKKIQILRKEAARDPLNNDGEWKLIRADDRTFLDFKQSVLKYNGGIPPLTSARY
                     KFFEPAGLVQGGAILGLDNDKSDSSSKEDANCDLDTSPNETATPFIGVASRSRCPSVI
                     TYYGIERHDLWVFTPVYR"
     gene            4120..4311
                     /locus_tag="VGB76_16855"
     CDS             4120..4311
                     /locus_tag="VGB76_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100618.1"
                     /translation="MATKKAGSKKGGGSSKGGGSSKGGGSSKGGGGGSKKGGGGGSKK
                     GGGGGSKKGGSSGGGSKKR"
     gene            4530..4985
                     /locus_tag="VGB76_16860"
     CDS             4530..4985
                     /locus_tag="VGB76_16860"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY0100619.1"
                     /translation="MINVALVEPEIPPNTGNIARLCAATRTPLHIVGVAGFRLDDRAV
                     RRAGLDYWPEVQLSRHRDLDALRDALPAARFVYLTTKARRSYTDWRYTTGDCLIFGRE
                     TRGLPEEVLRANASNCITIPMLNPNIRSLNLATSVAIVLYEALRQTRAF"
     gene            5179..7011
                     /gene="typA"
                     /locus_tag="VGB76_16865"
     CDS             5179..7011
                     /gene="typA"
                     /locus_tag="VGB76_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY0100620.1"
                     /translation="MTEERKDIRNIAIIAHVDHGKTTLVDAMLKQAGTYRENEHIIER
                     VMDSMDLERERGITIMAKNTAVRYGDVKINILDTPGHADFGGEVERVLKMVDGVMLLV
                     DAAEGCLPQTRFVLRKALELRLPVIAVVNKIDRQDARPAEVVDEIYELFLDLDATDAQ
                     IEFPILYAVSRDGIAKKNLTDESNDLRPLFDQIIETIPAPRALRQDSLQLLVANLDYN
                     EYVGRLAIGRIFSGEIKQNETVAIIKRDSSIQKMRVSKLYAFEGLKREAVERAGVGDI
                     VALAGMEGIEIGETISSADNPRALPVIAVDEPTISMIFSVNNSPFAGREGKYVTSRQL
                     RERLDRELLSNVAIRVEETETPDQFKVSGRGELQLAILIEMMRRENYELQVSKPQVIT
                     QQRDGKTFEPIELVVVDCPEEFIGVVTEAMGRRKGQMTKMVNHGTGRVRLEFETPSRG
                     MIGFRNEFLTETKGTGLLNTMFLRWGEWQGAMRGRATGSLVSDRMGETTTFALFNLQE
                     RGTMFARPGQKVYEGMIVGENARAVDLDVNVIKEKKLTNMRAASADEAMRLVPVKELS
                     LEQALEFIADDELVEVTPETIRLRKRVLPANLRPKKKESEAKQA"
     gene            complement(7086..7550)
                     /locus_tag="VGB76_16870"
     CDS             complement(7086..7550)
                     /locus_tag="VGB76_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100621.1"
                     /translation="MSSRARCLSLLLLAGLYTFTLVTPSRAQQIAHQTDAVRSSPLDV
                     LRSAQTIFIRTKTAYFKPAALEQSLLDRTEVQSWELVFTREEANADLIIEVDRKLFTN
                     KFVYSVIDPRTNTVLISGKIGSLGGTVEDQIANGFVKRLQRFRTQAAPARFR"
     gene            complement(7782..7940)
                     /locus_tag="VGB76_16875"
     CDS             complement(7782..7940)
                     /locus_tag="VGB76_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100622.1"
                     /translation="MSERQDDTTEPRERDAPTERESRPQNYYYDDGTGYEVYDPAQEE
                     EDEEEAAG"
     gene            7966..8835
                     /locus_tag="VGB76_16880"
     CDS             7966..8835
                     /locus_tag="VGB76_16880"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEY0100623.1"
                     /translation="MCRFLAYAGAPLLLADLLYRPVNSLIMQSYKARERAEPLNGDGF
                     GVGWYVPEVDPTPCVQRTVMPAWSNRNLQSLSVKVRASCLFAHVRAASPGMAVIESNV
                     HPFVYDRFMWMHNGAVANFERIKRRLRESLKDEFYNMVMGTTDSEHAFALFLNCLHVP
                     FGEAQSDNLRAAVVETIALLNEWTLEAGVVEPSHYNFAVTDGQSIVVSRYSSAEGIAG
                     NSLHISRGRRFECLPDGLCDMHTVKAEEKAAAVVVASERLTDDRDDWLDVPDNHTVTV
                     TPDLQVTVEPINL"
     gene            8854..>9970
                     /locus_tag="VGB76_16885"
     CDS             8854..>9970
                     /locus_tag="VGB76_16885"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEY0100624.1"
                     /translation="MNISINRRALAALSVALLLLLPQTNNAQQGTPQPSKRFELTVDS
                     IMRGAALVGYEPTSVRWSQDGGRVYFRWKAASEPRLKELDTYVVNRDGTNLRKLSEEE
                     AKNAPPASGELSKDKRWTVFSDEGDIYLYDHARGARRQVTRTVETETNPHFTRDQTRI
                     YFTRQNNLYLLSLEGGLLEQLTDIRTGGGGGGQRQGGASESQQQRGTESQEVLKREER
                     ALLEAVRERAARREELEARRRARERRKPFNIAVGQVVSNLTLSPDGAYVIATVAEQPP
                     ATAKTTIVPNYVTESAYTEDIQSRTKVGDTQGRTRMFVISVETGDARNVEHGQRMEAA
                     PVVRTRTEQNATGQAQRERGAQAQGTPEQQQTQGQAQQ"
BASE COUNT         2074 a         3056 c         3047 g         1793 t
ORIGIN      
        1 cagatactcg aacggctctt gcagtcggtc gataacacgg tcggcacggt gggcggcgtc
       61 gccaacaccg cgctgcaacc gggcggcgtc gtggatcgca cggtcggcac ggtcggtcag
      121 accgtgaaca ccctcgcaca gcccggcggg ctgctcagtt cgaccgttaa cactctgggg
      181 cagactttgc agcgcaccgt agaccgcacg ggcaacatcg tcgagcgcac gctcgacacg
      241 gcgggcacgg tcgtcaacca gcgcacggtc ggcaacgtgt cgagtctgag cgtgctgcgg
      301 gaaacgacga acgccgccgg gcaggtgctc cggcaggtgc gcgacacgtc gggcgcggtc
      361 atcgaactca cgctcgacgc ggcgggcaga atcaccaact cacgcgtcgt cagccaggcg
      421 acgggcacga acccgagaca gtaaaaggct gcgaatttaa gaggaacggg cggatgggca
      481 ggcgcgaaac tgcccgtccg cccgtctgtt ctgtgggttc tgcaattttt atagtttctg
      541 caacttgtac gcgccttgcg cgtttaactc gtgcgtcgcc gcgtcgggct tccgcgacgt
      601 tgcgcgcccg cgctgcccgc gttatgcttt ccgtatctgg ttcaagtgaa gctgcccgac
      661 cctcgcttga cgattttcgt ttgtttaaga gacgttaaaa gtagtgattg aaaatctcaa
      721 cctcgcttcc aacccctttc gcaatcggac gctgccgtgg acggtggcgg ccatcatcgc
      781 ctgcgtctcg gtggtcgcct tgatctttac catcagcagt ttccggcaga cgcgcgaccg
      841 cgcaagctcc atcgaacggg acgtgcgcgg cctgcgcacg gaagaggctt cgctgcgcgc
      901 caaggccgac gccgtgacac aggcgctcac gcccgaccaa ctgcgcacgc tcgaagccgc
      961 gcacacgctc atcgaccagc gcaacttctc ctggtcgcgc ctgttcgcag acctcgaagc
     1021 ggttctgccg cagggcgtga gagttgcgcg catcggtgtg cgcgatgtgg cgcatagcgg
     1081 cggcgcggag ttggacttgg caatcgtcgc gcgcacgccc gcagacatca cggggatgct
     1141 cggcacgatg gcgcaaggcc gcaccttctc cgccgacctg ctcaccgaaa acaagagcga
     1201 caagggcatc gaatcaactc tgcgtgtgcg ctacacgccg ggcgcgtcgc gtcccgccac
     1261 gcccgatgtt gtcgagagcg cgtcaggcag cagtagcggc gcgagtgaga acatgatcgc
     1321 cggagcgtcg aataattccg tcgcttccgc accgggggag acgcgatgaa agagacacgc
     1381 ccagacgccg gagcagaaga caaacgccgg acgatttcgc ccgcacagat tcgcgcacgc
     1441 ctcgccgggc ggcgcacgac gcgtcccacg ggcgcgatcc gcctcccgga gatcatcgcc
     1501 ctctccgtcg ccatcctgtt gctgctcggc gccgcgtgcg cctatttctt tctgctcgta
     1561 ccggagcgcg cgcgccttgt tgctttagaa aatgagcgcg cgcagttgca ggcgaagttg
     1621 cgtgcttcta cggaaggcgt ggagcgcaac gagagcacgc aggcgagcgt gggaaagatc
     1681 gtctccagcc tgaacgagtt cgagacgagc gtgctggtcg cacgcgagcc gcaaaccagc
     1741 acggctgtca tcgcagagtt gaacgacaag acgcgtcgcc acaatctctc gcgcgcgcag
     1801 ttctccttca gccacctcga cgaatttgtg ccgggacaga ccaaccggca gcaatcgaac
     1861 cgtcggagcg gagccggagc gggcaccgaa tcgaagcgcg agagcgtctt cccaaccatt
     1921 gacatcagcc tgaccatcga aggaccgtac ggcaatctgc gacgcttcat acgcgacatc
     1981 gagcagagcc gccgcttcat cgttatcaac ggcatccaac tcgaaggcgt ttcggacacg
     2041 ggcagcgcgg cgcgtgccgc gctcgtcagt ctgcgtctcg acatgtcggc gcacttccgt
     2101 cgcagcggtg cggcggtagc gagcggcgca acgtccgtgc ccgccgcgac atctcagtga
     2161 cgttcatcgc ccggaaattt ttatggcact ctttgaaggt aaaacacctg cggaacgtaa
     2221 caagatgatc gcggccatcg cgttcggcgc gctcgcgctc ctcttgctcg ggcgcatgtt
     2281 cttcggctcg gacgataagc cgccgccccg cgccgccggt aaagctaaag cgcgccccac
     2341 gagcggcacc acgccgggcg gcgatggcgc aaacggcgcg caacctgctc cggctgatga
     2401 actcatacag atgccgcgcg aactgaacat cgagcgcgtg agttataacg tgcccgatgc
     2461 ggggagaaat atcttcgctt tctacgttcc gcccgtgaag ccgacgccga atcccgcaga
     2521 ggtggcggcg actccgccgc ccacgcccga accgacgccg ccgctgatgc tcagcgcgat
     2581 ctctccttcg agcgtcatgg cgcgcacggg cgactttgcc ctgcaaatca ccggcgataa
     2641 gttcacaccg caatcgcgcg tctacgtgga cgggcaggaa ctccccacta cttttgcgag
     2701 cgcgcaacaa ctctcgacca acgtgcccgg cgtgctcatc aatgcgcccg gctcgcgcac
     2761 catcgtcgtc cgcacgcccg acaactcact ctattccaat cagacgatgc tcaacgtcgc
     2821 cgcgccgccc acgccgcagt acacctacgt cggcatcctc ggcagtccgc gttacagcga
     2881 caaggcgatc ttgaaggcgc agaacaacaa cgaactcatc accgtgcagc gtggcgacac
     2941 ggttgccgga cgcttcaaag taacaagcat ttccacgcgt gccgtcgagt ttacggacgc
     3001 gcagttgcag atcaagcaca ctctgcctta cgtcgagaac cgcgccaccg gcggcggctc
     3061 tcccggtagt ccctcgcccc gcttcaatcc tccacctaaa cccgcagacg acgacggcga
     3121 cgacgatgaa cctcaagatt aaagatcaac gtcagacacc gccgcctgct gttgctgttg
     3181 cgacgcagcc cggcacgggc gcacgcgcgg gcgagcgcgg ctacacgctc gtcgctctgc
     3241 tcgccttgat gacgatcctc gccctcgcgc tcaccgctgc cgcgccgcgc ctgcgccagc
     3301 aagcgcaacg caatctcgaa cgcgaagcca tcgcgcgcgg cgaagaggtt gccgaagcca
     3361 tcgccctctt ccaactcgcg aagggcacgc cgccgaagag catggatgat ctgctcgaag
     3421 gcgtcgaacc gtttgcgggg gcgttcaaga agattcaaat tctgcgcaaa gaggcggcgc
     3481 gcgatccgct caacaacgac ggcgaatgga aactcatccg cgccgatgat cgcaccttcc
     3541 tcgatttcaa acagagcgtg ttgaagtaca acggcggcat cccgcccctg acgagcgcgc
     3601 gttataaatt tttcgaaccg gcggggctgg tgcagggcgg cgcgatcctc ggcctcgaca
     3661 acgacaagag cgacagcagt tcgaaggaag acgcgaactg tgatctcgat acttcaccca
     3721 acgagacggc cacgcccttc atcggcgtcg ccagccgcag ccgttgtcct tcggtcatca
     3781 cctactacgg catcgaacga cacgacctct gggtcttcac tccggtctat cgctaaccac
     3841 gcttccgtca taattcaact cgcaggagag tgcgcgttaa tgatcgttaa cgcgcactct
     3901 tcgcatctgt gatcctctct tcttcatctc atactcactc gctcattcat catgcgcgca
     3961 aaaaaagtta acgattgata attaagttat tgacaactga tcgctttatg tttataagtc
     4021 caacatgttg tgcgctgttt accctaggcc acaagatgaa aaacgacaca ctatcttgtg
     4081 gtcttaatct acaaagagaa aaaggagcaa cgaagactta tggcaactaa gaaagctggt
     4141 tcgaaaaagg gtggcggctc atcaaagggc ggcggctcat ccaaaggcgg cggctcatcc
     4201 aaaggcggcg gcggcggctc gaagaagggc ggcggcggcg gttcgaagaa gggtggcggc
     4261 ggcggctcga agaagggcgg cagcagcggc ggcggctcga agaagcggta gtactagtaa
     4321 gtccaaacgg agtaactcaa caaccttcac tagcgcacac aaaacattcg gtcttcgaca
     4381 aagaccattc aaaaggacgg cggttgctac cacagcacgc cgtcctcatc ttttacaata
     4441 tcagccggag gctagagaat ggagtttggt taaaaacagg aaccgtcttc tactgatctc
     4501 tgacctctga cttccaatct ctacttctta tgatcaacgt tgcgctcgtc gagccggaga
     4561 taccaccgaa cacaggcaac atcgcgcggc tctgtgcggc gacgcgcacg cccttgcaca
     4621 ttgtgggtgt ggcggggttt cgtctcgatg atcgcgcggt gcgtcgcgcg gggctggact
     4681 actggcctga agtgcagctc tcgcggcatc gcgacctcga tgcgctgcgc gacgcgttgc
     4741 ccgccgcgcg cttcgtctat ctcacgacga aagcccgccg tagctacacc gattggcgtt
     4801 acacaacggg cgattgtttg atttttggcc gggaaacgcg tggtttgccc gaagaggtgc
     4861 tccgcgccaa cgcctcgaat tgcatcacga tcccgatgct caaccccaac atccggagcc
     4921 tcaatctcgc tacctcagtc gccatcgtgc tctatgaagc gttgcgccag acgcgtgcgt
     4981 tttaaccctc aaccgtcagc acgcgcccac gcaaaagcga gccgcaaagt aacttcaaag
     5041 taaattcaat tttaaccgat ctttcaggcg agaagcttga gccacgctga gcgcaaggat
     5101 aattcggcgg cgcgtggtat catctacggt ttgatgtggc gcgcataatc gcgctgtttt
     5161 gatctatgta gaggaactat gactgaagaa agaaaagata tacgcaacat cgccattatc
     5221 gcgcacgtcg atcatggcaa gaccacgctc gttgacgcga tgctcaagca ggccgggacg
     5281 tatcgtgaga acgaacacat catcgaacgc gtgatggatt cgatggactt ggaacgcgaa
     5341 cgcggcatca cgatcatggc gaagaacacg gcggtgcgct acggcgacgt gaagatcaac
     5401 attctcgaca cacccggcca cgcggacttc ggcggcgagg ttgaacgcgt gttgaagatg
     5461 gtagacggcg tgatgcttct tgtcgatgcg gcggaaggtt gtctgccgca gacgcgcttc
     5521 gtgctgcgca aggctctgga gttgcgcctg cccgtgatcg cggtcgttaa taagattgat
     5581 cggcaggacg cgcgaccggc ggaagtggtg gatgagattt acgaattgtt tcttgaccta
     5641 gatgcgaccg acgcgcagat cgaattcccg atcctctacg cggtgtcgcg tgacggcatc
     5701 gccaagaaga atctaacaga cgagtcgaac gatctgcgtc cgctctttga tcagatcatc
     5761 gagacgatcc ccgcgccgcg cgcgctgcgt caagactctc tgcaactcct cgtggcgaat
     5821 ctcgattaca acgaatacgt cgggcggctc gccatcgggc gcatcttctc cggcgagatc
     5881 aagcagaacg agacggtcgc catcatcaag cgcgacagtt cgattcagaa gatgcgcgtc
     5941 tccaagctct acgctttcga gggcttgaag cgcgaagcgg tggagagggc gggcgtgggc
     6001 gacatcgtcg cgctcgcagg gatggagggc atcgagatcg gcgagacgat ttcgagcgcg
     6061 gacaacccgc gcgccttgcc cgtgatcgcg gttgatgaac cgacgatctc catgatcttc
     6121 agcgtgaaca attcgccgtt cgcaggtcgt gaaggaaaat acgtcacgtc cagacaactg
     6181 cgcgagcggc ttgatcggga actcctgagc aacgtggcga tccgcgtgga agagacggag
     6241 acgccggatc aatttaaagt ctccggtcgc ggcgaactgc aactcgccat cctgatcgag
     6301 atgatgcggc gcgagaacta cgagttgcag gtgtcgaagc cgcaggtcat cacgcagcag
     6361 cgcgacggca agacgttcga gccgatcgaa ctcgtcgtgg tggattgccc ggaggagttc
     6421 atcggcgtcg tgacggaagc gatggggcgg cgcaaagggc agatgacgaa gatggtcaac
     6481 cacggcacgg ggcgcgtgcg tctggaattt gagacgcctt cgcgcgggat gatcggtttt
     6541 cgcaacgagt ttctgacgga gacgaagggc acgggcttgc tcaacacgat gtttctgcgc
     6601 tggggtgagt ggcagggcgc aatgcgcggt cgcgccaccg ggtcgctcgt ctcagaccgc
     6661 atgggcgaga cgacgacctt cgcgctcttc aacttgcagg agcgcggcac gatgttcgcg
     6721 cgccccggcc agaaggttta cgaaggcatg atcgtcggcg agaacgcacg cgccgtcgat
     6781 ctcgacgtga acgtgatcaa ggagaagaag ttgacgaaca tgcgcgcggc ttcagccgac
     6841 gaagcgatgc gcctcgtgcc cgtcaaagaa ctctcgctcg aacaggcgtt ggagtttatc
     6901 gccgacgacg aactggtcga agtgacgccg gagacgattc gcctgcgcaa gcgcgtgctg
     6961 cccgcgaacc tcaggccgaa gaagaaagag tcggaagcaa agcaggcata gacgcgcgcg
     7021 gcacgcgttt gcagggaaag aacgggagcc gggtcagagt cttgagattt cagtaacttt
     7081 gaatctcatc tgaatcgtgc gggagcagcc tgcgtgcgga atcgttgcag acgcttcacg
     7141 aagccgttgg caatctgatc ttcgaccgtg ccgccgagcg agccgatttt tccactgatg
     7201 aggacagtgt tcgtgcgcgg gtcgatcacg ctgtagacga atttgttggt gaagagtttg
     7261 cggtcaacct ctatgatcag gtcggcgttt gcctcttcgc gcgtgaagac cagttcccag
     7321 ctttgaactt cggttcgatc aagcagcgac tgttcgagag cggcaggctt gaagtaggcg
     7381 gtcttcgtgc ggatgaagat cgtctgcgcc gagcgcagca cgtcgagggg acttgaacgc
     7441 acggcgtccg tttgatgtgc aatttgctgc gctctcgacg gcgtgacgag cgtaaacgtg
     7501 taaagacctg cgagaagcag caggctcaag cagcgggcgc gagatgacat gaagtgttgt
     7561 ctcctgaaac gtcggcgata ttgggaggga gatgttaacg ccgcgtcgcg tgccctcgcc
     7621 ccgcctgcta tgacacttcg caaattgtca taccagactc gacggttcga gcgcgctgcg
     7681 cgcgggttga agcgtctgcc gcattcgcac gcgccccggc gcaggttcgc acgcgcgcaa
     7741 tgtaaaggct cgcttctgcc caaacgccaa ttcgcacgca ctcaaccggc ggcctcctct
     7801 tcgtcctcct cttcctgcgc cggatcgtaa acctcatagc ccgtcccgtc gtcgtagtaa
     7861 taattttgcg gcctgctttc gcgttcggtg ggcgcatcgc gttcgcgtgg ttctgttgta
     7921 tcatcttgcc gttcgctcat ttattctccc tgagagcatc aaattatgtg ccgctttctc
     7981 gcttacgcgg gcgcgccgct cttgctggcg gacctattat accgcccggt gaactcgctg
     8041 atcatgcaga gctacaaggc gcgcgaacgc gcggagcctt tgaacggcga cggcttcggc
     8101 gtcggttggt acgtgccaga agtagacccg acgccgtgcg tgcagcggac ggtgatgccc
     8161 gcctggtcga atcgcaactt gcagagcctc tctgtgaagg tgcgcgcgtc gtgcctgttc
     8221 gcgcacgtgc gcgcggcgtc gccgggcatg gccgtgattg aatcgaacgt gcacccgttc
     8281 gtctatgatc gcttcatgtg gatgcacaac ggggcggtcg caaatttcga acgcatcaag
     8341 cggcgtctgc gcgagagttt gaaagacgag ttttacaaca tggtgatggg cacgaccgac
     8401 tcggagcacg ccttcgcgct ctttctcaac tgcctgcatg tgccgttcgg cgaagcgcag
     8461 agcgataatt tgcgcgccgc cgtcgtcgaa accatcgcgc tcttgaacga gtggacgctt
     8521 gaggcgggcg tcgtagagcc ttcgcattac aacttcgccg tgaccgacgg ccaatcaatc
     8581 gtcgtctcgc gttacagttc agccgaaggc atcgccggta actcgctgca catttcgcgt
     8641 ggcaggcgtt tcgaatgtct gcccgatgga ctgtgcgaca tgcacacggt caaggccgaa
     8701 gagaaggcgg cggcggtcgt cgtcgccagc gagcgtctga ccgacgacag agacgactgg
     8761 ctcgacgtgc cggacaacca caccgtcacg gtcacgccgg atttacaggt aacggttgaa
     8821 ccgatcaatc tttaagtagt acgaaaacgg gatatgaaca ttagcattaa ccgccgcgcg
     8881 ctcgccgcgc tttcagtcgc cctcctcctg cttctccctc aaacaaacaa cgcgcaacag
     8941 ggaacgccgc aaccgtcgaa gcgtttcgag ttgacggttg acagcatcat gcgcggggct
     9001 gcgctggtgg gctacgagcc gacgagcgtg cgttggtcgc aggacggcgg gcgcgtctat
     9061 tttcgctgga aggcggcgag cgagccacgc ctgaaagagt tggacaccta cgtcgtgaac
     9121 cgcgacggca cgaacctgcg caagctctcg gaggaggagg cgaagaacgc gccgcccgcg
     9181 agcggcgagt tgtcgaagga taagagatgg acggtcttct cggacgaggg cgacatctat
     9241 ctctacgacc acgcgcgggg cgcgcggcga caggtgacgc gcacggtcga gacggagacg
     9301 aacccgcact tcacgcgcga ccagacgcgc atctatttca cgcgccagaa caatctctac
     9361 ctgctctcgc tcgaaggcgg cctgctcgaa cagttgacgg acatacgcac gggcggcggc
     9421 ggtggcggtc agcgtcaggg cggcgcgtcc gagtcgcagc agcagcgcgg cacggagagt
     9481 caggaagttt tgaaacggga ggagcgcgcg ttgctcgaag ccgtccgcga gcgtgcggcg
     9541 cggcgcgagg agttggaggc gagacgccgc gcgcgcgagc gacgcaagcc cttcaacatc
     9601 gcggtcgggc aggtcgtctc aaacctcacg ctctcgcccg acggcgcata cgtaattgcc
     9661 accgtcgcgg agcagccacc ggcgaccgcg aagacgacca tcgtgccgaa ctacgtcacg
     9721 gaatcggcct acacggagga catccagagt cgcacgaaag taggcgacac gcaggggcgc
     9781 acgcgcatgt tcgtcatctc ggtcgagacg ggcgacgcgc gcaacgtcga gcacgggcaa
     9841 cgcatggaag ccgcgcccgt cgtccgcacg cgcaccgaac agaacgccac cgggcaagcc
     9901 caacgcgagc gcggcgcaca ggcgcagggg acgccggaac agcagcagac tcaaggacag
     9961 gcacagcagc
//
LOCUS       DASWNS010000493        27830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_227982, whole genome shotgun sequence.
ACCESSION   DASWNS010000493 DASWNS010000000
VERSION     DASWNS010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 27830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27830
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227982"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..809
                     /locus_tag="VGB76_16890"
     CDS             300..809
                     /locus_tag="VGB76_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100625.1"
                     /translation="MAANLAIIVVALTLTTVVFKRYLLPDTQKNVPASQSQISIGDKL
                     SIANVDWRTSNNTLVLVLQKGCRYCDESAAFYRRLIKGLDGRSDVRLLAVLPQDAGES
                     KEYLNDLGVTVGEVVQSPPSAFGVRGTPTLMLVNHNGEVQDIWQGKLTGEQESKVSER
                     LHLTLAAAF"
     gene            1129..1809
                     /locus_tag="VGB76_16895"
     CDS             1129..1809
                     /locus_tag="VGB76_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100626.1"
                     /translation="MKPRINGSLLIFVALLFCASFLVVRNVLTQGAANSTQATASLRE
                     RAKRNGNVIVKVFPHSLKRYDSLNDLTKESSAVILGTVISQASSLKLPAENSVTTNYL
                     VRVNNVLKGDLSKESLVSVNSPGGKVALEGGAYAEMAMPDYWKTPHVEKTYVLFLTPG
                     DGNDYGILGGPQGLFEATASGIKPQGLLTDELFRSHHGMSLKKFTHRVRSAIENHERA
                     ALRGQSPN"
     gene            1872..2903
                     /locus_tag="VGB76_16900"
     CDS             1872..2903
                     /locus_tag="VGB76_16900"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0100627.1"
                     /translation="MSRIRLHSFSLIGLIITAASILVGGYVSAYKLPETDEKGLQAVS
                     EKLIECVRGGDIACVQRELSGGVSPDARDQKDKELSTVLMVAVVADHADILDLLISKG
                     ADVNAASTKGRTALMWAAWKGMTRGAQALIKKGANVNAKDSSGNTALTIAAWKGHADI
                     VAELLDGGADVNARGRNNDTALLTAIEEKHDHLAAVMIEKGADPNLADNFQRTPLIFA
                     AEKGSLNNVRALLKAGAEVNHRDLRDQTPMLLAIKQSNLKMVSEMLEANADVNLFTRL
                     GDTPLMVAAELGDINIIRMLLEKGADIEARDAQGRSALVRAKLVGQTRSVNLLASEGA
                     RTSVKNWPY"
     gene            3029..3565
                     /locus_tag="VGB76_16905"
     CDS             3029..3565
                     /locus_tag="VGB76_16905"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEY0100628.1"
                     /translation="MLSATQHQLLALFATTFVALIFIASGGAKLLRPRLFVDAVRDFK
                     IVPDSASKPVAYLLPYLETCVGLFLLLDQYRPIFLTVAVIMLAAFTLAITVNLARGRT
                     LISCGCFGSHEKTELTWLLVARNLLFVAVAIVGLIHGGSFTNVALAERVVVILSTGAL
                     ITSYYLSALIVRFRQLEF"
     gene            3611..4231
                     /locus_tag="VGB76_16910"
     CDS             3611..4231
                     /locus_tag="VGB76_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEY0100629.1"
                     /translation="MNSIWLVSYFVLWLVVILLTLAVVLLARQVGLLYRRLGPAPARM
                     ENEGPPIGSLPSQVEALTLDGRQVAVGGAQGKPQLLVFVSPTCVTCNGLAPALRAAWK
                     SERHSIDFLTVCMTDDETLVRSFVNRNKLQEVSCIMSLELSRQYNIFNPPHAVLLDRG
                     GAVIAKGVVNNLDHIESLLNAAETGHPSVESYIHHLESANHAVAVE"
     gene            5091..5720
                     /locus_tag="VGB76_16915"
     CDS             5091..5720
                     /locus_tag="VGB76_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100630.1"
                     /translation="MVGTIIPIGYGDTHRTQYIRKPAVVLAHLAGTTVGATAFGVAVG
                     TLGMLLKGGLALAGYEQLMILVTGTLALLYGGHELKIFRLPAPQSSWQVPEKWRAEMS
                     PGWASFFYGAGLGPGLFTAIPFTTFYLILAWVLVSASPTLGALCFAVYGVGRATPVFL
                     MFYLSEGSKQVFQLSREADLWQPVVHLFSGLALCVAGTCLIVGAFTAAG"
     gene            5849..7078
                     /locus_tag="VGB76_16920"
     CDS             5849..7078
                     /locus_tag="VGB76_16920"
                     /inference="COORDINATES: protein motif:HMM:NF018172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HEY0100631.1"
                     /translation="MRSIFNIAKSKKLVVGLKSVGIRFSGRVAPIFCILLAVFCAPCD
                     VSAGVQSGLVYVLDSGGGNGKSQVLVVDPEQKRIVRRFGTEHNPDMAVAPDGGRLYVA
                     YSVNENGKLDIYDTVSGNLLRTVENKDRWLPTSHLYISHMALSPDGKWLYIFKYEIQT
                     DLYYIATFDTAQERFLPEIAPLPECLNAIMSPSAEGRQLAVMCTSSDDLRFVKVMDDG
                     KGRITRLPLRLRGRTTRFGLPIGLSNVLPTQSSYKIVAGDGSFFEVSGGTKSIKRLGV
                     LDAKGRKITYSESPSLIADKEDWIGGKWLPIQYPAIAPKSGKVYLGMGQLADLRMGRW
                     TYNTIGVFDIDTLNRVSLLETKHRLFSISVNPTGSRLYGVDPLGKRLVVFDTGSGSEI
                     GLVEDLGESPMLVIAAP"
     gene            7169..7612
                     /locus_tag="VGB76_16925"
     CDS             7169..7612
                     /locus_tag="VGB76_16925"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0100632.1"
                     /translation="MRQVTLCALLLLFISAWSIGQMSNASAVASAVQSRVSGVVKKIT
                     ARQLEGFISSGSPVTIIDVRLPKHYESSQTKIKGAIRITPSDLAWRLNEIPRHKQVVL
                     YCACPDEATSLRLGERLKEGGYKDVSVLKGGWDAWVQNGGQQTQK"
     gene            7790..9244
                     /locus_tag="VGB76_16930"
     CDS             7790..9244
                     /locus_tag="VGB76_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100633.1"
                     /translation="MMLAIIFTALVHDAYLLFRYPVAVGVDGYYYVLQVENLHDRGTL
                     FFPNSTPLVIYLLVALKFFVSSTVLAIKVGSFLFHLALAAGVFALLKTLTRSRMHAMC
                     GTLIIGASSVHLFMLSEYIAHSGGLALLVWGAYFTVRAVRTGNTWWKGGAVIALVLAS
                     LSHRSCIFMIAVLAVMLFIYHLIMTPVRVNRRRTLTAGAVGLAFFISPLVFAGQTFVA
                     LSPEVRDESLASPGLSFGRVAILEKCLLLLCSLLFLVISFKHSRRRQTFVYKVLGTIS
                     VLSLLMTLNPFLDRSGGWLSASERISALAYIQLAILLPGLFWLLSPSVGRLARPLCVA
                     SVICFSAFSLVISLPYGLRDDYLHDRIRLIKSLTEQRQALTHLRRVIAPHGEQFVITY
                     SLGVPAQQRVTPGEDLSTTHWLLQHFDRRNLNSVEGVIVLDEQQSTFIVPDKHLDAIL
                     RAMPLWEGLELTKANPHLIDRILSGTNTYRLPVR"
     gene            9846..10646
                     /locus_tag="VGB76_16935"
     CDS             9846..10646
                     /locus_tag="VGB76_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100634.1"
                     /translation="MNSPEKTYVQEIAKGLGYLGTWLPTVTLQLGDYGVLFDKYKFIR
                     IGNLSDMNVPFGIRQGRSRASMQYESNNAVTMSAKVAADLNLPNAPITTKVEFSFSRE
                     NAVVFKANKAQSVSIQDQAALGSAVMGLLNNGKWNKKHRIITELVSAASTTIIVASAK
                     SSSLELTAQGDVSSSLDIANASLGFMATRKAAMAYSDIAVQGLTPMFRTSGVRTTGMW
                     WWKEDDFQPFGLKGVNALDVLTDEEVADHPEALYFGLDGFEELLSDAV"
     gene            10750..12138
                     /locus_tag="VGB76_16940"
     CDS             10750..12138
                     /locus_tag="VGB76_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100635.1"
                     /translation="MVLLDSRISSVTRRAASLAAEIEQWLCYAEQGNVFEKNSSQLEA
                     LDIFMARVTAQLKNDSSAISGSMRPEPSNAADILHKIEAIERELFEGYFIWAYFRSKL
                     EQRFVPQFADALLTTDLVSYNCYRTTLDNANRLGIEHKLGLRDYPLTYFLEDYPSPMT
                     WPRDVRQRGLAGRTLAVPVIGIPWDHIATPWEWLSLNHEVAHAIDYDLGIPSKELSGH
                     LQGKSDRASVWQLWMSEIFADALAILLAGPAFVSYLANSLTVPGDKATSLAKVGLHPP
                     PYLRVVLNVQLAQDLIPGRMVQNYVSNLLNQWEQIYGKQQSGLSDYLNDLDDIMRVLK
                     TNLNTLKDGQGVAHSLMELIGFGESDFENQLAGRDQFLAGGEVVNHMSLRHVLGAAYM
                     ATEEHINSHGSLDSRFADGLAIRIKAAIKVKAPAGQLPLRTEASKRYLRDLADAFLAD
                     SIAGGINDQQIS"
     gene            12119..14644
                     /locus_tag="VGB76_16945"
     CDS             12119..14644
                     /locus_tag="VGB76_16945"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0100636.1"
                     /translation="MISKYLDFELKVYVEGGQIEIDIDGRPFGYSLPERLKIPFCLAP
                     VSYYSLTQNPANIADAGHSLFESVFCEEVKTAFASYLNSAKEFEGVRVCIQTPSNVLS
                     ESIWEILCDKINPSVNFLALDPKTPIIRTPRTGWLGYDRDLQAPLRMLIVLASPRLLQ
                     KIKPDEEKASLESALKDAVEQGLIDIAYLGFDEPTEANFDTLQEHLANQMRPYDVVHI
                     IGHGLLEAGMEGKVALVQEDDGKMQSVNASSLANLFRSRRVILVVLQSCQSGAVDSSA
                     TSFSGVAQLLVASGVPAVLAMQGIIDQDVAQDFIGKLYKQWISSGSRFEDALTQARQS
                     VFQKYQARVAPWAIPVLYIVPGFQLVLKPPASPHEPRPQDALSVGTTERQVDAALPKQ
                     VRLGRETTLLVLIRTPTAAGLREKLGAEPRDYEAAPEDVLVSPTFDVTFGEDEKTGRL
                     RPGIVKIVIETKDFDIERTEENVQIRPQGDSVLCIFPLTPRKEGAARLLLSVLDNSEQ
                     KLTLTQLQLKSEVRSGEIFEIEYKVSDAAQVSDERATQLAYSNSLHEMKVNSELLRHF
                     SQRTLAQREQLKEYTISLHQQLRQDFLSSENQNAFRNHIIRIATFLSEQPVESETQGT
                     TQVVVALVQENLERLMNKQQYLNSTWNSRYSGERIDEAIESLVGSYRDMIGYLWHTLG
                     RDTWKALLTATVAEGEGISPCPQCESTKIRYMLFDAYMRPYFGLRFACAACGYPQRHW
                     GNRGTDYEENLRNLYQISRRYRDECAEQGEQAARIMSRLEVIPDKHKTTEAFYRISRE
                     LEGLQRQFRFAEYFYHEVRAYIDDPHYFRLLELESILNQDDEY"
     gene            complement(15286..15492)
                     /locus_tag="VGB76_16950"
     CDS             complement(15286..15492)
                     /locus_tag="VGB76_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100637.1"
                     /translation="MPTPEAIVEKMLQMAKVGRDDVLYDLGCGDGRVVSHNYGMGDWE
                     PQEVAEVEVGTSTHYIYSWVVPDR"
     gene            complement(15657..17048)
                     /locus_tag="VGB76_16955"
     CDS             complement(15657..17048)
                     /locus_tag="VGB76_16955"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0100638.1"
                     /translation="MIGRISVKAGSGGSPVPTLSLKDAVAITIGIVVGAGIFRTPSLV
                     AANAGSESAVLLIWLVGGAVTLIGALCYAELATAYPHPGGDYHYLTRAYGKPLAFLYA
                     WSRITVVQTGSVALLAFVFGDYASQILSLGEHSASIYAVLIVLALTGLNVAGVRQGSR
                     TQNWLTSVEVLSLLLVIIAGLALTATPNAPAAATQPTGGQAPGAALGLALVFVLLTYG
                     GWNEAVYVSAEVKGPRRNVARALLLSIGVITGLYMLVNWAYLRGLGLEGLAGSEAAAA
                     DLMRRAAGEGGARFVSLLVAISALTSANATIITGARTNYALGRDFRLFDFLGRWHESA
                     NTPTNALLAQGGIALMLVFAGTLAREGFATMVEYSAPVFWLFFFLAGLSLIILRVREP
                     EVERPFRVPLYPLTPLLFCAACLYMLQSSLAYTGAGALAGVLVLLTGVPVLLLARQRQ
                     QHLAPVKYMEKRA"
     gene            complement(17161..17757)
                     /locus_tag="VGB76_16960"
     CDS             complement(17161..17757)
                     /locus_tag="VGB76_16960"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0100639.1"
                     /translation="MRKATFNRLHLAGVVVCAAFTVACAYAQQKDVKKDVPYVPTPQT
                     VVDEMLKLANVSKGDVVYDLGCGDGRLVITAVKNFGARRGVGVDIDPQRITESNENAK
                     VAGVTDRVRFAVQNLFETDFKEATVVTLYLLPEVNLKLRPKLLRELKPGTRIVSHAFS
                     MGDWEPEKTVTVEPGGQRLYLWTVPAKAAPATTTGNAP"
     gene            18105..18236
                     /locus_tag="VGB76_16965"
     CDS             18105..18236
                     /locus_tag="VGB76_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100640.1"
                     /translation="MTLRLVAVEAQQTPYPVFRRHHFLLAFERRADGTANQVVILDR"
     gene            complement(18251..18955)
                     /locus_tag="VGB76_16970"
     CDS             complement(18251..18955)
                     /locus_tag="VGB76_16970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0100641.1"
                     /translation="MSELVAMLDEPCGALLLAADENRATPAPEDEKYRASDYALARQA
                     ASGDLDAFEEIYRRHAQRMYGVCLRMTGNAAEAEDLTQDVFVHIFRKLGAFRGESALT
                     TWLHRVAVNQVLMHFRKRRSRPESITADGDMPPEVERGTVDPNRMPVIDRIALDEAVA
                     QLPPGYRTVFVLHDVEGYEHREIAQMLGMAVGTSKSQLHKARMKLRSLIRRAQPERQA
                     VSDERRTNAEGTVEAV"
     gene            complement(19192..19971)
                     /locus_tag="VGB76_16975"
     CDS             complement(19192..19971)
                     /locus_tag="VGB76_16975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0100642.1"
                     /translation="MIVDDEPVARARVRRLLAEEPDIEVVGESRNGFEALTHIRELAP
                     DLLFLDVQMPGKDGFQVLEDLKAGETPIVIFLTAYDKYAVRAFEAAALDYLLKPFDEE
                     RFARAVARARAQLAQPSQLRKVGDASTQMDEGRAETADALVGADARHLERIIIRTGGR
                     VLFRRADEIDWIEAYGNYVRLHIEKAAYLLRETISGLEERLDPQKFARIHRSTLVQMD
                     RIREVRPHVNGQWKVILRDGTQLTMSRRYRRRLPATLAEAL"
     gene            complement(20062..20598)
                     /locus_tag="VGB76_16980"
     CDS             complement(20062..20598)
                     /locus_tag="VGB76_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100643.1"
                     /translation="MRYKLSLRQIFSLVLLSATFSALVVTGINFIDRPPYVASAAAES
                     APTPGIADPSSATDEQNNIDVYRALSPGVVFITSSSAGGRNLWDYGGRSGTGSGSIID
                     KQGHILTNEHVVARATRISVSLGGERSAPGCPQAGGRTALGKASACATRAAGSNSSTT
                     GSTVSNCATFRAAGRKSL"
     gene            21151..21435
                     /locus_tag="VGB76_16985"
     CDS             21151..21435
                     /locus_tag="VGB76_16985"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0100644.1"
                     /translation="MTVPETTSAEAFWRYAGGTCLFESRGEAWRDVKAWLTARYHIDL
                     RMNEARRLLRETKMGVVDVALEVGYANPSHFARLFRRETGLSPSNYRRQR"
     gene            21959..22108
                     /locus_tag="VGB76_16990"
     CDS             21959..22108
                     /locus_tag="VGB76_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100645.1"
                     /translation="MYTAGDVRVEEREDPKIVDPTDAVIWLCSDASSFVVGHALVIDG
                     GQTVA"
     gene            22385..23539
                     /locus_tag="VGB76_16995"
     CDS             22385..23539
                     /locus_tag="VGB76_16995"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0100646.1"
                     /translation="MKHKAILLVVTALVLSAAFASRAQDTQAGKPSQTILRAGSQPSG
                     KGPAENFTGNVTVAPLFPADASASYSGAYVTFEAGARSAWHMHPAGQRLVVTAGAGRT
                     AEWGGRVQEIKAGDVILCPPGVKHWHGASPNTAMTHLALTNSVNNKNVEWMEKVTDAQ
                     YNAGVRPMNTTHALSAKQRSIIPIAAFTATGDQAKLRTALNEGLEAGLTVNEIKEILV
                     QMYAYAGFPRSLSGIQTFMAVMDERQAKGIKDQVGRDAAPLPTGMNRDEYGAKVRAKL
                     AGQEVIPPPAGYQLFTPVIDTFLKEHLFADIFARDILDHRSRELATVAALSSMTGTRS
                     QLQFHLGAAMNVGITEAQLRDFISVLAAKVGERESESAGEVLSAVLTTLK"
     gene            23665..24489
                     /locus_tag="VGB76_17000"
     CDS             23665..24489
                     /locus_tag="VGB76_17000"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0100647.1"
                     /translation="MKDDLDETSGTELTADEEALLKEMPTLTDVGQGRRTFMGQTLAG
                     GLGTFALMLLEKERALAALSTSPDAMFAPVPALENPVRVVLRINGATRTLELDSRVVL
                     LDALRERLELTGTKKGCDQGQCGACTVLVDGRRVLSCLTLAAQVEGREVTTVEGLAKG
                     DELHPVQAAFIKYDGFQCGYCTPGQICSAVGMLTEVRHGDVSHVTEDVTVTTTRLTDD
                     EIRERMSGNICRCGAYPGIVAAIREVHSGRESARTWRFVEEEELARIRKEEANEAV"
     gene            24476..25462
                     /locus_tag="VGB76_17005"
     CDS             24476..25462
                     /locus_tag="VGB76_17005"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY0100648.1"
                     /translation="MRPFKYTRISDPNAATRALAANPRAKFLAGGTNILDLMKEDVER
                     PDELVDLTRLKLAEIKAAGGGVSIGALAKNSDTANHPLIRRGYPLLTQAIVAGASGQL
                     RNMATNGGNLMQRTRCQYFYDTTMPCNKREPGTGCGALEGLNRIHAIFGWSDKCVATY
                     PGDMANALYALDAVVRVRGARGRERTIPVGEFHRLPGDTPEKDTNLAHGELIVAIELP
                     RSNFAKNSYYLKVRDRASYAFALVAVAAALELDGRRVRQARVVLGSVAHKPWRSMEAE
                     AALAGMPASEESFRRAAEAALAGAKPLAHNAYKVELGKRAVVRALMRAAKLA"
     gene            25479..27710
                     /locus_tag="VGB76_17010"
     CDS             25479..27710
                     /locus_tag="VGB76_17010"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY0100649.1"
                     /translation="MPRYIGKEMSRVDGVAKVTGRAKYAAEFRAPNLAYGFIVLGSIA
                     KGRITNIDTREAERAPGVVRVFTHLNAPKLGPKTASEDSPPSSREPTPREERDKSFRA
                     LQSDRIYFNMQPVALVVAETYEQARYAARLVRVSYNAEKHTTDTESVRDRGRFPPQGP
                     PPKPRGNPEAAMKSAPVKIEAEYRIPIEHHNPMEPHAAVAVWQGDKLTIFDKTQEVYG
                     VRAHLAAAFGVPEENVQVISPYVGGAFGSSLRPNYYPSLTAMAARELKRPVKVVYTRT
                     QMYTGHGYRPYTIQKIALGAGRDGKLSAMIHEAVHNTSTFEEFSDATTAFTRQVYACP
                     NLSAPLRITATDLNTPTWMRAPGAVSGMFALECAMDELAYALKIDPLELRLINYAERD
                     PESGKPWSSKALRECYRLGAEKFGWKNRKMEPRSMRDGRLLVGWGTATGVWGAGQMPA
                     TARITLKADGTAHVASATSDIGPGTYTVITMIAAEYLGLKPEQVQFELGDTKFPRAPA
                     QGGSWTTASVGSAVYGAALAIAARLLTLANRDADSPLKAAAGSDVEMLDGRLRLKSDP
                     SRFINVSEVMRRNNLAEIAETFASRPSKEREKYGLLAHGAQFIEVKVDPDLGQVRVTR
                     AIEVTACGKIINPKASHSQEIGGVVWGIGMALQEATEIDHRYGRIMNPNLQHYHVPVN
                     ADVHEVETMFVEEEDKVVNPLGVKGMGELGMVGIPAAIANAVFHATGKRIRELPITPD
                     KLL"
BASE COUNT         5818 a         8023 c         8311 g         5678 t
ORIGIN      
        1 aaacagcttt cctttttcta ttgtatttgg aaaaaggtcg ccttatcatc cacgctgcct
       61 tatccaataa ctagtacgtg aattcaaaat ccagtctgac gcattactgt ttccgcgacc
      121 gttgatacag gagatcagcc ggaacaggag cggtagtgca cacatctctt cggcctctta
      181 acttcgggca gcttagggta tggctgagcc tggcgcacat gtccaatatt caactgcttc
      241 cgcgcactgc gctttcatga gatacagcga caatgggcac tacgtataaa aaagtagagt
      301 tggcagccaa tctggctatc attgtcgtcg cgctgacgct gaccactgtt gtcttcaaac
      361 gttacctgct gccggatacg cagaagaacg tcccggccag ccagtctcag attagtatcg
      421 gtgacaaact ctctatcgct aacgttgact ggcggacaag caacaacacg ttagtgctcg
      481 tgctgcaaaa ggggtgtcgt tattgcgatg agagtgccgc cttctaccgg cggttgatca
      541 aaggcttaga tggcaggtca gacgttcgcc tgctggcagt gctgccacaa gatgcgggcg
      601 agtccaaaga gtatttgaat gatctggggg taactgtcgg cgaggttgtg cagtctccac
      661 cttcggcctt cggggtgcgg ggcacaccca ccctcatgtt agttaaccat aacggtgagg
      721 tgcaggacat ctggcagggg aaattgaccg gtgagcagga atccaaggtt tccgagcgcc
      781 tgcatttgac cctcgccgct gcattttgag gtagcaacac tgtcctgacg aaggagaact
      841 tatgcgaccc gtgtcagaca tcacgcacca cttctccacg tatttcaacg gcctcgcgtc
      901 acgaatcttg ctgctggccg ccatcagcgc tgtcttgctg gccgtgcaat tgacctcgcc
      961 cccgccagca agcgcagact gcggcgattg caccccgggg acgtgttgcg attacgagtg
     1021 ctcacttgag tgttgctact gggataggta cgagaattat tgtgtgtccc agtgtttcat
     1081 ctgcttcaat atctgtagct cgtgttgcat ctgaggaggt cccgtctcat gaaacccaga
     1141 atcaacggct cgctactcat cttcgtggca ttactcttct gtgcgtcctt tctggttgtc
     1201 agaaacgtgt tgacacaggg cgcggcgaat tcgactcagg ccacggcctc tttgcgggag
     1261 cgggcaaagc gtaacggtaa tgtcatcgtc aaagtcttcc ctcattcgct gaaaaggtac
     1321 gacagcctca atgatctgac aaaagagagt tccgccgtca ttctggggac tgtgatatcg
     1381 caggccagca gtttgaaact gcccgccgag aactccgtga ccacaaatta cctcgtcagg
     1441 gtaaacaacg tgctcaaggg ggacttgtcg aaagagagtc tcgtcagcgt taactcgccc
     1501 gggggcaagg tggccttaga gggtggtgcc tatgctgaaa tggcgatgcc cgattattgg
     1561 aagacaccac acgtcgaaaa gacttacgtg ttattcctta cccccggtga cgggaacgac
     1621 tacggaatat tgggcggccc gcaaggcctg ttcgaggcca cggcgtcagg catcaagccg
     1681 cagggactat tgacagacga actgttccgc tctcatcacg gcatgagtct gaaaaaattc
     1741 acccatcgcg tacgctcggc cattgaaaac catgagcgcg ccgctctccg aggtcagtct
     1801 cccaactgac ccgttgaagg ggtggctcgc atcatagaca tgacccacaa cttcggaaag
     1861 gagccgtcgt tatgagcaga attcggctgc actcgttcag cttgattggc ctgattatca
     1921 ccgcggcgtc catcctcgtc gggggctacg tgtctgcgta caaactgcct gagacggacg
     1981 agaaagggct tcaggcggtc agcgaaaagc tcatcgagtg tgtcaggggc ggcgatatcg
     2041 cttgcgtgca gcgcgagttg agtggcgggg ttagtcccga cgcaagagat caaaaagaca
     2101 aggagctttc aaccgtcctg atggtggccg tcgtggcgga tcacgctgac attcttgatc
     2161 tactcatcag taagggagcg gacgttaatg ccgcgtccac caaaggcaga accgcattga
     2221 tgtgggcggc ttggaagggg atgaccagag gcgcacaggc gttgataaag aaaggcgcca
     2281 acgtgaacgc gaaggacagt agcgggaaca cggcactcac gattgccgct tggaagggac
     2341 acgccgacat cgtggcagag ttgttggatg gcggcgctga tgtcaacgcg cgtggccgga
     2401 acaacgatac tgcgctgctg acagccatcg aggagaagca cgatcacctc gccgccgtca
     2461 tgattgagaa gggtgcagac cctaatctcg ccgataactt tcagcgcacg ccgcttatct
     2521 tcgccgctga aaaagggagc ctgaacaatg tcagggcact gctcaaggcc ggtgccgaag
     2581 tgaaccatag agatctgcgt gaccagacgc cgatgctcct agcgattaag caaagtaact
     2641 tgaagatggt ttcggagatg ctggaggcca atgccgacgt gaatctgttt acccgtctag
     2701 gcgacactcc tctgatggtc gcggcggagc taggcgacat taacatcatc aggatgctgc
     2761 ttgagaaagg cgcagacata gaggcgcgcg acgcacaggg gagatcggca ctcgtgaggg
     2821 cgaagctcgt aggccagacc cgcagcgtga acctgctcgc ttcagaggga gcgagaacat
     2881 ccgtcaagaa ttggccttat tgaccttatt gaatggagcg cggcattcag ccgtcgtacg
     2941 tttcccgatc acttgtaacg cccaaccgaa atcgtcagca actttaccag tcagattcag
     3001 agactgaatg attagcggct gaatccctat gttgtccgca acccaacatc agcttctggc
     3061 actcttcgct acgacgtttg tcgccttgat attcatagcg tccggtggtg cgaagctttt
     3121 aagaccgcgt ttgttcgtcg atgctgtccg cgacttcaag atagttcccg acagtgcatc
     3181 taaacccgtc gcgtacctgc tcccgtacct tgaaacctgc gtcgggctgt ttctcctgct
     3241 cgaccagtac aggccgatct tcctcacggt cgccgtcatt atgctggcgg cttttacgct
     3301 cgccatcacg gtcaacctgg ctcggggacg gacacttatt tcgtgcggct gcttcggatc
     3361 gcatgagaag actgaactca cttggctgtt ggtcgcccgc aacctcttgt tcgtcgccgt
     3421 ggcgatagtt gggttaatcc atggcgggag ctttactaac gtagcgctag cggaaagagt
     3481 ggtggtgatt ctatcgaccg gcgcgctcat cacttcttac tacctgtcag ccctgattgt
     3541 cagatttcgg cagctcgaat tctgacctgc gcactggtcc agacatgccc gcccaatacc
     3601 ggataagtat atgaatagca tctggctcgt atcctacttc gtcctctggt tggtcgtcat
     3661 tctgctgacg cttgcggtcg tcctcctcgc acgtcaagtc ggtttgctct acaggcgtct
     3721 cggccccgcg cccgcacgga tggagaatga aggcccgccg attgggagcc tgccgtcgca
     3781 ggtggaagca ttgacccttg acgggcgaca agtggccgtc ggcggcgcgc agggcaaacc
     3841 gcagctcctt gtgttcgtct ccccgacttg tgtcacctgc aacggacttg cccctgcgct
     3901 tcgcgccgcc tggaagagtg agaggcacag cattgacttc ctaaccgtgt gtatgaccga
     3961 tgacgaaacg ctcgtgcggt cgttcgttaa taggaacaag ttgcaggagg tatcttgcat
     4021 catgtccctc gaactgtctc ggcagtacaa catcttcaac ccaccccacg cggttttgct
     4081 agaccgggga ggggctgtca tcgcaaaagg cgtcgtcaac aacctagatc acatcgaaag
     4141 tttgctgaat gccgctgaga cagggcaccc gagcgtggag tcttacatcc atcatctaga
     4201 gagcgccaac catgccgtgg cggttgaata agtcgcgctc cgaaacctct gaagagggag
     4261 acaagagcgg agacgttatg gaacatgata atcagacacc agccctgctc gataccttcg
     4321 tggaaaaatt cctgagggag cgtggccgaa ccatctcgcg gcggggaatc ttagcccggc
     4381 tgggtaaatt ctcgctagcc attctaggag tttcattgtt gcccaacctg ccactagatc
     4441 gcacctttgt cgtggatgca caaggggatt gctgtcactg ggcattgtgc ggcatcaatg
     4501 gctacctatg cactcccggc ggggcgacgg caggctcctg tcctagcggg acgacggtgg
     4561 ggccgacttc ctggtcaaaa tgttgcacaa gcggggagtt gtgcggcgag ggaggtacga
     4621 cagtcgagta ttgggactgt tgcgcgagca ccagtactgc tgcgacttct gtgcgggggg
     4681 cacagtgtgc gcataacccg agcgcaggcg gcgcatggtg cccttcggga agcccttttt
     4741 acggctgcac ctacgccctg caacgcggcc cttgcagcag tccggcgtat aacccttgct
     4801 agtctacagc gcaaccgctg cacgcgcatg gcgaccggac agcgcaagag cagcgggcgc
     4861 tttgacacag ggcgaataac ttaatggaag ctttaaggat tggcaaaatc aggatcaccg
     4921 gcctagctca actccggcgt ctggtcttgt tgctcgccgt gtgcatggct agtgctgccg
     4981 tggcttactg gttcccgcag gtcatgagtt ggaatcacgc tgcgctggtg gtcttcgccg
     5041 taagctttct ggcaggcttc accgctttat ggtcgccttg aggtttctcg atggtgggca
     5101 ccatcatccc tataggttat ggggacacgc atcgaacgca gtacataagg aaacccgcag
     5161 tcgtcctggc gcacctggcg ggcacgaccg tcggggcaac cgctttcggc gtcgccgtcg
     5221 ggacgttagg aatgctgctc aaaggagggc tggccctggc gggatatgaa caactgatga
     5281 ttctcgtcac gggcactctg gctttacttt acggagggca cgagttgaag attttccgcc
     5341 tacctgcgcc gcaatcgagt tggcaggttc cggaaaaatg gcgtgccgaa atgtcgccgg
     5401 gatgggcctc gtttttctac ggtgccggac tgggacccgg attgttcaca gccattccgt
     5461 tcacgacctt ctatctcata ctggcgtggg tgttggttag cgcgagtcct accctcggtg
     5521 ccttgtgttt tgccgtctac ggagtgggga gggcgacccc ggtattcctg atgttctatc
     5581 tgtccgaagg aagtaagcag gtgttccagc tctcgcggga agcagacctt tggcaacctg
     5641 tggttcacct cttcagcggc ctcgcattgt gtgtcgcagg aacgtgtctc atcgtggggg
     5701 catttaccgc agccgggtaa gtcgcacgca ggtgtgcgcg ggttgagtca caccggggca
     5761 gcgcaccggg gcggggtcaa aggctgatgg aaaggcatag acgaggggcg tcccgcgtgt
     5821 cctcgcttgg aatgaaatgg agggttttat gcgcagtata ttcaacatcg ccaagtcgaa
     5881 gaaactcgtg gttggtctga agtcggtcgg cattcgtttc tccgggcgcg tcgctccaat
     5941 tttctgcatt ctgctggcag tcttttgcgc gccctgcgat gtctcggcgg gcgttcagtc
     6001 aggtctggtt tacgtgcttg attcgggtgg ggggaatggg aagtctcagg ttctcgtggt
     6061 ggaccccgaa caaaagcgaa ttgtgagaag attcggcacc gagcataacc ccgacatggc
     6121 ggttgcgccc gatggtgggc gcttatacgt ggcttactcc gtgaatgaaa acggcaagct
     6181 ggatatctat gataccgttt ccggcaactt gctgcggacg gttgaaaaca aggaccgctg
     6241 gcttcccaca tcgcacttgt acatttcgca catggccttg tcgcccgacg gaaagtggtt
     6301 gtacatcttc aagtatgaaa tacaaaccga cctctattac atcgccacct tcgacacggc
     6361 gcaggagcgc ttcctgccgg agattgcacc cctgccggaa tgtttgaacg caatcatgtc
     6421 gccgtcggca gagggcaggc aactagcggt gatgtgtact tcgtcggacg atttgcggtt
     6481 cgtcaaggtc atggacgatg gcaaggggcg catcacccgc ctgccgctgc gattgcgcgg
     6541 taggacgacg cgcttcgggt tgcccatcgg gctgagcaac gtcttgccca cacagtcaag
     6601 ttacaagatc gtggccggcg acggttcgtt cttcgaagtt agcggcggga cgaagagcat
     6661 caaaaggctc ggcgtgctag atgctaaggg caggaagatt acttacagcg aatccccaag
     6721 tctcatcgcc gacaaggaag actggatagg cggcaaatgg ctgcccattc agtatcccgc
     6781 catcgcaccc aagagcggca aggtgtatct cgggatgggg caactcgctg acctgcgcat
     6841 gggcagatgg acttacaaca ccattggtgt tttcgacatc gacaccctca accgtgtgag
     6901 cctgctggaa acgaagcatc gcctcttttc catctcggta aacccgacgg gcagtcggct
     6961 ctatggggtt gatccgctcg gtaaacgctt ggttgtgttc gacacaggca gcggcagcga
     7021 aatcgggttg gtggaggacc tcggggagag tccgatgctg gtcatagccg cgccctgata
     7081 ttgggacacc actcgcgccc cgcttccgac ggaagcggtc gaagcgcgcc acttttcgtt
     7141 ttaacgcccg tgcgcttgag gcgaacacat gagacaagtc acgctatgtg cactactgtt
     7201 gctcttcatc tcggcatggt cgattggtca gatgagcaat gcctcagccg tcgccagcgc
     7261 ggtgcagtcc cgcgtgagtg gggtcgttaa gaaaatcacc gccagacagc tcgagggttt
     7321 catctctagc ggaagccccg tcaccattat cgatgttcgc ttacccaagc actacgagtc
     7381 cagccaaact aaaattaaag gggcgattcg tattacgcca agcgatttag cgtggcggct
     7441 taacgagatc ccacgtcaca agcaggttgt cctctactgc gcttgtcctg atgaggctac
     7501 cagcctgcgc cttggagaga ggcttaaaga gggtggctac aaggacgtga gtgtgctcaa
     7561 gggtggatgg gacgcgtggg tgcaaaacgg cgggcaacag acacagaaat agcacatgcc
     7621 ggtcgcgcct tgcgccacgt ccgcatttct tactccgaat ttcttactcc gataagtccc
     7681 tgtatccgga aaccacgacg cacatcctta cacggggtca caaaccgcta gcgtgaaatt
     7741 caagcgcccg catcaacatc tatcagcccg cacacgcatt tggacgttaa tgatgctcgc
     7801 catcatcttc acggccttgg tgcacgacgc atatttgttg tttcgttacc cggtcgcggt
     7861 aggcgtggac ggttattact acgtactgca agtcgaaaac ctacatgacc ggggcaccct
     7921 ttttttcccc aacagcactc cacttgtcat ctatcttctg gtggcattaa agttttttgt
     7981 ttccagcacg gtgttggcaa tcaaggtggg aagcttctta ttccacctcg cacttgctgc
     8041 tggagtgttt gcgctcctca agaccttgac caggtcaagg atgcatgcga tgtgtgggac
     8101 gctcatcatc ggcgcttcga gcgttcatct cttcatgctg tcggaataca tcgcccattc
     8161 cggaggcctc gctctattgg tttggggggc gtacttcacc gtccgcgctg tccgaacggg
     8221 taacacgtgg tggaagggcg gtgcggtgat tgcgctagtg ctcgccagtc tctctcatcg
     8281 gtcgtgcatt ttcatgattg cggtgctggc ggtcatgctc tttatctacc acttaataat
     8341 gactcctgtt agggtgaacc gccgccggac gctgacggcg ggggcggtcg ggctggcatt
     8401 tttcatctcg cccttagtct ttgccgggca gacattcgtc gctttatccc cggaggtaag
     8461 agatgaatca ctggcatcac ccggcctttc gttcggacgc gtcgccatcc tcgaaaaatg
     8521 cttactcctg ctgtgctctc tgctgtttct ggtcatcagt ttcaagcact cccgacggag
     8581 acagacgttc gtctacaaag ttctcggcac catctcagtt ttgagcctgt tgatgacgtt
     8641 aaatcctttc ctagaccgga gcggcgggtg gttgagtgca tcagagcgca tcagcgcatt
     8701 agcctacatt cagcttgcca tcctcttgcc cggcctgttc tggctgctgt cgccctcagt
     8761 gggacggctc gccagacctc tttgcgttgc gtctgtaatt tgtttctcgg cgttcagcct
     8821 cgtgataagc ctgccatacg gactacgcga tgactatttg catgaccgca taaggttaat
     8881 caaatcactg acggaacaac gtcaagcctt aacccacctg cgaagggtaa tcgctccgca
     8941 cggcgagcag tttgtcatca cttattcgct cggggtgcct gcgcaacagc gagtgacgcc
     9001 gggtgaagac ctctcaacca cacactggct gctccaacac tttgatcgta ggaacctaaa
     9061 ctccgtcgaa ggggtcatag ttttggatga gcagcaatct actttcattg tgccggataa
     9121 acatctggac gctattttgc gagccatgcc gctctgggag ggacttgagc tgaccaaagc
     9181 caacccacac cttatcgacc gaatcctgtc gggcactaat acctatcgcc tgcctgtcag
     9241 gtgaggcttc gcctccaacc acgataactc gcggcggaag ccgacagtga aagcaagccc
     9301 tcctttgggt acacgacgga gccgcttgtg aacccttacc gacgtgggtg caagtttaat
     9361 ttacagccca tttcgaccct cccggcaaga ctctttacat tataagcatc gggcatagcc
     9421 tgctcagcgg ttagtaatcg cagcaacata gaggtagtgc tttcgactct ttacaaaata
     9481 gttgttttgt aaagagttga cctctactga agcgtacttg catctttcga gggagtccaa
     9541 actgcctgac tgtaggtgca cttctgttta taaatattga cattcgtatt cacgcaggca
     9601 tatctttccc acgtttttgc cgctcggttt tgctagcgcc cgctttcctt cgttgacctc
     9661 acttccgtat agatcaacgt caaggagggt ggaatgaaat ttttaacatc aactcgaatg
     9721 gacaacggat taatctgttg agctgctctg attaagacgc aaacatcagt ttcttcaagg
     9781 gagacgggcc gctcggctga aatcttccgg aaacttcatt gtccaagctt cagtaggagg
     9841 ttcgtatgaa tagcccggag aaaacctatg ttcaagagat tgcgaaagga ctcgggtatc
     9901 ttggcacctg gctccctaca gtaacgctcc agttagggga ttacggtgtc ttatttgata
     9961 agtataagtt tattcgaata ggtaatctca gtgacatgaa tgtcccgttc ggcatccgac
    10021 aggggcgatc acgagcgtcg atgcagtatg agtcgaataa tgccgtaacc atgagcgcca
    10081 aagtggcggc tgaccttaat cttccgaacg ctcccatcac cactaaggtc gagttctcgt
    10141 tcagcaggga aaatgccgtc gtctttaaag ctaataaagc tcagtccgtg agcattcaag
    10201 accaagccgc cctcgggagc gcggtgatgg gcctcctgaa caacggcaag tggaacaaaa
    10261 aacataggat cattacagaa ctcgtcagtg ctgcctcgac cacgatcatt gtcgcgagcg
    10321 ccaaatcaag cagtctagaa ttgacggcac agggcgacgt ttcatcctca ctggatatcg
    10381 ccaatgctag tctgggtttt atggctacgc gcaaagccgc gatggcttac tcagacatag
    10441 ccgtccaggg gttgacgccc atgttcagga cttccggggt tcggacgacc gggatgtggt
    10501 ggtggaagga agacgatttt cagccgttcg ggctaaaagg ggtaaatgct ctcgatgtgc
    10561 tgaccgatga agaggtcgct gaccaccccg aggcgctcta ttttgggttg gatggcttcg
    10621 aagaactcct cagcgatgcc gtttgagtcg gcgtgctgag agccgttttt ggcttcctta
    10681 atattcccca accccttcgc ttcgtcgcgg tccgctgcga agcctccact cttagggtgg
    10741 caggacacga tggttctact agactcaaga atcagctcgg tcacgaggcg tgcggccagt
    10801 ctggcggcgg agatcgagca atggctgtgc tacgcagaac agggaaacgt gtttgaaaaa
    10861 aacagttctc aactggaagc actcgacatc ttcatggcaa gggtgacggc ccaactgaag
    10921 aacgacagct ccgctatatc cggctcgatg cggcccgaac cttcgaacgc cgcggacatt
    10981 ttacacaaga ttgaggcgat tgagagagag ttgttcgagg gttatttcat ctgggcatac
    11041 ttccgcagca agttagaaca gcgattcgtg ccgcaattcg cggacgcact actgactaca
    11101 gacctcgtat catacaactg ctacaggacg acgttagata acgcgaacag gttagggatc
    11161 gagcataaac tggggctacg cgattaccct ttaacctact tcttggagga ttacccgtct
    11221 ccaatgacgt ggcccagaga cgttcgccag cgggggctgg cgggtcggac cctagcggtg
    11281 cccgtcatcg gcatcccctg ggaccatatc gctaccccgt gggaatggct gtctctcaat
    11341 cacgaggtgg ctcatgccat agactatgac ttgggcatac cctcgaaaga gctttcaggc
    11401 catctccaag ggaaatccga tagggcgtct gtctggcaac tctggatgag cgagatattt
    11461 gccgatgcac tggcgatact gctcgccggg ccggcattcg tctcctattt agccaactct
    11521 ttgacagtgc cgggggacaa ggctacttca ctcgcaaagg ttggccttca cccaccaccg
    11581 tatttgcgag tcgtgttgaa cgtacagctt gcgcaggact taatcccagg caggatggtg
    11641 cagaactacg tttccaatct cctgaaccag tgggaacaga tatacggaaa gcagcagtca
    11701 ggtctgagcg attatctcaa tgatttagac gacatcatga gggtcctgaa gacgaacctc
    11761 aataccttaa aagatgggca gggggtagca cacagcctga tggaactcat cgggttcggc
    11821 gagagtgatt tcgagaatca actggcgggg cgagaccaat ttctggctgg tggggaggtc
    11881 gtcaatcaca tgtcattacg gcatgtcctc ggcgcggcct acatggctac ggaagaacac
    11941 atcaattcgc atggaagtct cgacagccgt ttcgctgacg gtctagcgat ccggatcaag
    12001 gccgcgataa aggtgaaagc tccagccggg caattaccgc tccgcaccga agcgtctaaa
    12061 cgttacctgc gagatttggc agacgctttc ctcgccgatt caatcgccgg aggtataaat
    12121 gatcagcaaa tatcttgact tcgaattgaa ggtatacgtg gaaggcggac agatcgagat
    12181 agacatcgac ggcaggccgt tcggttatag cctgccggag cgtttgaaga taccgttctg
    12241 cttggcccca gtcagttatt acagcttaac ccaaaatccc gctaacatcg ccgacgccgg
    12301 ccactccttg ttcgagtcgg tcttttgtga agaagtgaag accgcgttcg catcttactt
    12361 aaacagcgcg aaggaattcg agggcgtcag ggtttgcatc cagaccccgt ctaacgtact
    12421 gagcgagtca atctgggaga ttttgtgcga caagataaac ccgagcgtca acttcctggc
    12481 cctcgaccct aagactccga tcatcaggac accgcggact ggttggctag gatacgaccg
    12541 cgatttacag gcccccctgc gaatgctgat cgtcttggct tcgccgcggc tcctacagaa
    12601 aataaaaccg gacgaagaaa aagcatctct ggagtcagcc ctgaaagatg ccgtagagca
    12661 gggactgatt gacatcgctt acttaggttt tgatgagccg acggaagcca atttcgatac
    12721 gctgcaagaa catctcgcta atcagatgcg tccgtacgac gttgtgcaca tcatcgggca
    12781 cggcctcctg gaagcaggca tggaaggtaa ggtagccctc gttcaggagg acgacggtaa
    12841 aatgcagagt gtaaacgctt ccagtctggc taacctgttc cgctcgcgca gggtcatatt
    12901 ggtcgtgctg caatcttgcc agagcggcgc agttgattcg tcggcaactt ccttttcggg
    12961 tgttgcgcaa ctgttggtgg cttctggagt gcccgccgtg ctcgccatgc aggggatcat
    13021 agatcaggac gtggctcagg attttatcgg gaaattgtat aagcagtgga ttagcagcgg
    13081 ttcgcgcttc gaggatgctc taacccaggc aagacaaagt gtgttccaga aatatcaagc
    13141 gcgcgttgcc ccatgggcca ttcccgtact ctatatagtg cccggtttcc agttggtttt
    13201 gaagcccccc gcctcccccc acgaacctcg accacaggac gccctgtcgg tggggacgac
    13261 ggagcggcaa gttgacgccg ccctgcccaa gcaggtccgg ttgggcaggg agactacatt
    13321 attggtgctg atccgcacgc ctactgcggc gggattaaga gagaaattgg gcgcggagcc
    13381 gcgcgattac gaagccgcgc ccgaggacgt gctcgtgtca ccgaccttcg atgtaacctt
    13441 cggggaggat gagaaaacgg ggaggctccg gcccggcatc gtgaagattg tgattgagac
    13501 aaaggacttc gacatcgaac ggacagaaga aaatgttcaa atccgacctc aaggggatag
    13561 cgtgttgtgc atcttcccct taaccccgcg gaaggaaggg gccgcaaggc tgcttctcag
    13621 cgtactcgat aattcggagc agaagctaac cctaacccag ttacagctta aatctgaagt
    13681 gcggagcggc gagatcttcg agatagagta caaagtatca gatgcagccc aagtgtcaga
    13741 tgagcgcgca acgcaactgg cttattctaa ctcccttcat gaaatgaaag tcaattccga
    13801 gcttcttcgc cacttctctc agcgaacgct cgcacagagg gaacagttga aggagtacac
    13861 aatatctctt caccaacaac taaggcagga ttttttgtct tccgaaaacc agaatgcttt
    13921 tcggaatcac atcattcgca ttgccacatt cttgagtgaa cagccggttg agagtgagac
    13981 tcaaggaact acccaggttg ttgttgcact tgtgcaagag aacttagaac gacttatgaa
    14041 caagcaacag tatctaaaca gcacctggaa ttctcgttat tcgggtgagc gaattgacga
    14101 agctattgaa tcgctggttg gtagctatcg agatatgatc ggttatctat ggcatacttt
    14161 ggggagggac acgtggaagg ctctcctgac tgcgactgtt gcggaaggcg aagggatatc
    14221 gccatgcccc caatgtgagt ctaccaagat acggtacatg ctctttgacg catatatgcg
    14281 gccatatttc gggctgcggt tcgcttgtgc tgcttgcggg tatccccaaa ggcactgggg
    14341 aaatcgcggg actgattatg aagagaatct tcggaattta tatcaaatca gccgacggta
    14401 tcgggatgaa tgcgcagaac aaggcgaaca ggcggcgcgc attatgagtc gcttggaagt
    14461 catccccgat aaacacaaga ccacagaagc attttacagg attagtcgcg agttagaggg
    14521 actccagcgc caattccgat ttgcagaata tttctaccat gaggtaagag cttatattga
    14581 cgaccctcat tattttagac tccttgaact ggaatccata ttaaatcagg atgacgagta
    14641 ttagatgtgc tattcggact gatcgtgtcc gttccatgac ctatacctca tgtcataatt
    14701 cagtcacgta ctctccggtc agactcttta caaagtggtc attttgtaaa gagttgactt
    14761 ctggtatccc ttcgcccggc tcatccaaac cgaataatct ttcatttgca ttggtatacg
    14821 aagaggccga acatgaatcc aatgcttatt cttcttcttt gcgtccggaa acgctgttgc
    14881 cgatgacctg aaggatgtct accaagcgat ggctatggaa gatgtggagc ggcagcatac
    14941 gcagtttgag gagccatgtt tcgccagtta tctcatgatt gctcagccgt ggcggactac
    15001 ctcaaaacga ccagccgggc ttaccgcgaa gcgttcgtcc gagggtacta cgaactctac
    15061 cggagccaga gaggcgacag cgccgcacgt taaggtgaag ggtaattcaa cggtcgcgga
    15121 acgggcgggg acgaacggcg tctccgcccg tttctcattg ccggtagccg cgacgtgcgc
    15181 gcatccagtc gagagcctgc gcggcgtgca tcagtcaacg gttgtaagag ccgcggcgcg
    15241 ggttgccgat gacgaggaag gcgaagggct gagagctggc agtcttcaac ggtcggggac
    15301 gacccacgag tagatatagt gcgtgctggt gccgacctcg acctcggcca cctcctgcgg
    15361 ctcccagtcg cccataccgt agttgtgcga gacgacgcgc ccgtcgccgc aacctagatc
    15421 gtagagcacg tcgtccctgc ctaccttcgc catctgaagc atcttttcga cgattgcttc
    15481 cggcgtcggc acgtaaggta cgtcgagctt agatcggtct accggcgttg cggcgacact
    15541 cggggcgggc gtcgcccgcg cttgggtgtt ggccgactga gttcagctgg tgccgccaca
    15601 cgataacgcg acgagtctgg cagcgagcat ccacgacttt gacagataat tgatcctcat
    15661 gccctcttct ccatgtactt gacgggggcg aggtgctgct ggcgctgcct cgccaacaag
    15721 agaaccggca cgccggtcag tagtaccaag accccggcca gtgccccggc gcccgtgtag
    15781 gcgaggctcg actgcaacat gtagagacag gccgcgcaga agagaagcgg ggtgagcggg
    15841 tacagcggca cgcggaacgg gcgctcgacc tccggctccc tgacgcgcag aattatgagc
    15901 gagaggccgg ccaggaaaaa gaaaagccag aagacgggcg cgctgtattc caccatcgtc
    15961 gcgaaaccct cgcgcgccag agtgcccgcg aagaccagca tgagcgcgat gcctccctgc
    16021 gcgagcagcg cgttggtggg cgtgttggcg ctctcgtgcc agcgcccgag gaagtcgaag
    16081 agcctgaaat cgcgccccag cgcgtagttg gtgcgtgcgc cggtgatgat cgtggcgttg
    16141 gccgaggtca gggcggagat ggcgacgagg aggctgacga accgcgcacc gccctcgccc
    16201 gccgcgcggc gcatcaggtc agccgccgcg gcctccgaac ctgccagtcc ctccagtccc
    16261 agcccgcgca ggtaagccca gttgaccagc atgtacaagc ccgtgatgac gccgatgctc
    16321 agcagcagcg cccgcgcgac gttcctgcgc ggccccttca cctccgccga gacgtagacg
    16381 gcctcgttcc agccgccata agtcagcagg acgaagacga gcgccagccc gagtgccgcg
    16441 cctggtgctt gcccccccgt cggttgcgtg gcggcagccg gggcgttcgg agtggccgtg
    16501 agggccagcc cggcgatgat gaccagcagc aggctgagga cttcgacaga tgtcagccag
    16561 ttctgcgtgc gcgatccctg tcggacgccc gccacgttca ggccggtcaa cgccaagacg
    16621 attagaacgg cgtaaatgga ggccgaatgc tcgccgaggc tgagtatttg tgaggcgtag
    16681 tcgccgaaga cgaacgcgag cagcgcgacc gagccggtct ggacgaccgt gatccgcgac
    16741 catgcgtaga ggaaagcgag cggcttgcca taggcgcggg tcaggtagtg gtaatccccg
    16801 cccgggtgtg gataggccgt cgcaagttcc gcgtaacaca acgcgccgat taaagtcacc
    16861 gccccgccca ccagccagat cagcagcacg gcgctttcgc tgcccgcgtt ggccgccacc
    16921 agcgacggcg tacggaagat gcccgcgccg acgacgatgc caatcgtgat cgccaccgcg
    16981 tccttgagcg agagcgtcgg cacgggactg cctccgctgc cagccttcac actgattctg
    17041 ccaatcattc aaccccccgg cacgaaagcg atacgacaac gccacatcga acaggccgaa
    17101 gcgggttgcg gccatcctgt gtccgcttaa attgcttacg aggccgtcgc ccggacgagg
    17161 ctatggcgcg ttcccggtcg tggtggccgg agcggccttc gccgggactg tccagaggta
    17221 gagcctctgc ccgcccggct cgacggtgac ggtcttctcc ggctcccagt cgcccatgct
    17281 gaaggcgtgc gagacgatgc gcgtgcccgg cttcaactcg cgcagtagct tggggcgcag
    17341 cttgaggttc acctcaggca gcaggtagag cgtgacgacg gtggcctcct tgaagtctgt
    17401 ctcgaagagg ttctgcacgg cgaaccggac gcggtcggtg acgccggcga ccttggcgtt
    17461 ctcgttgctc tctgtgatgc gctgcgggtc tatgtccacg ccgactccgc gcctggcgcc
    17521 gaagttcttg acggcggtga taaccagccg tccgtcgccg cacccgaggt cgtagaccac
    17581 atcgcccttc gagacgttcg ccagtttgag catctcgtcc acgaccgtct gcggcgtggg
    17641 cacataaggg acatccttct tgacatcctt ctgctgggcg taggcgcatg ccaccgtgaa
    17701 cgccgcgcac accacgacgc ccgcgaggtg taaccgattg aatgttgctt tccgcatcat
    17761 catcccccct cataaaagac taaagttggc agtgcgagcc atgatcttgg tgaaaattga
    17821 aaccggggac cggctgacga gtagggtctg tcgggtgatc ttagccccaa cggactgtcg
    17881 catcctcaat catcaacagg aacgcaggcg gctcggaaaa atactcgtca gcccttcgac
    17941 ggaaggctgg agccacttcc ttcgacatcc gtttcttttg aggtctattt tctgaatacc
    18001 ttcatttccg ggaaggggaa tttatatact gcttaaccac cgggcgtatg tccgttaggg
    18061 tacgcaatct tgtcagcttc tccgaaacat caggtgatga tgagatgact ctgaggttgg
    18121 tcgcggtcga agcgcagcag accccgtacc ccgtctttcg ccgtcaccac ttcttactcg
    18181 ccttcgagcg tcgcgcggat ggaactgcga atcaggtcgt catcctcgac cggtaagacc
    18241 gtctttttcg tcataccgcc tcgacagtcc cctcggcgtt cgttcgtcgt tcatcgctga
    18301 ccgcctgccg ctccggctgc gcccgcctga ttagacttcg cagtttcatc cgcgccttat
    18361 gcagttgcga cttcgacgtt ccgaccgcca tcccgagcat ctgtgcgatc tcccggtgct
    18421 cgtacccctc tacgtcgtgc agtacgaaga ccgtgcgata gccgggcggc aactgcgcca
    18481 ccgcttcgtc gagggcgatg cggtcgatga ctggcatccg gttcgggtca acagtcccgc
    18541 gctcgacttc gggtggcatg tcgccgtccg ccgtgatgga ttcgggtcgt gagcggcgtt
    18601 tcctgaagtg catcagcacc tgattgacgg cgacccggtg gagccaagtc gtcagcgcag
    18661 actcgccgcg aaacgcgccc aacttgcgga agatgtgaac aaagacatcc tgcgtcaggt
    18721 cttccgcctc cgccgcattg cccgtcatgc gcaggcagac gccgtacatg cgttgagcgt
    18781 ggcggcggta tatctcctcg aaagcgtcca gatcaccgga ggccgcctgt cgcgccaacg
    18841 cgtaatcgct ggcgcgatat ttttcgtcct cgggcgcagg cgtcgcccta ttctcgtccg
    18901 ccgcgagaag taaggcacca cacggttcgt ctaacatagc gaccagttca ctcatgactt
    18961 ctgacacggt tcgcgccccc gcacacgtgt gctaccgcca caggtgtcga gacgccaatt
    19021 aagaactacg gacgccgatt gagagcgtgg cgtcgccaag tttaggtgtt tattgatgag
    19081 ccgggcgctg cttgcccgtc ggcggtttgt gacgtgtggt cggcggtagg tgatgaacgg
    19141 tcggcgcgaa gagagttaat gaagtttccg tgggtcgagg cgaaggagta ttcagagagc
    19201 ctcggcgagt gtcgcaggca ggcggcggcg gtagcggcgg ctcatggtca actgcgtgcc
    19261 gtcgcgcagg atgactttcc attgcccgtt gacgtggggg cgcacctcac ggatgcggtc
    19321 catctggacg agcgtggagc ggtggatgcg cgcgaacttt tgcgggtcga ggcgctcctc
    19381 aaggccactg attgtctccc ggagaaggta cgcggctttc tctatgtgca ggcgcacgta
    19441 gttgccgtaa gcctcgatcc agtctatctc gtcggcgcgg cggaagagga cgcgcccgcc
    19501 cgtgcggatg atgatccgtt cgaggtgccg cgcgtccgcg ccaacgagcg cgtccgccgt
    19561 ctccgcacgc ccctcgtcca tctgcgtgct cgcgtcgccc actttcctga gttgtgacgg
    19621 ttgagcgagt tgcgcccgtg cgcgcgcgac ggctcgcgcg aaacgctctt catcgaaagg
    19681 cttcagcagg taatccagcg cggcggcttc gaaggcgcgc acggcgtact tgtcgtaagc
    19741 cgtcaggaag atgacgatgg gggtttcccc ggccttgagg tcttcgagaa cctgaaagcc
    19801 gtctttgccc ggcatctgca cgtcgaggaa taagaggtcg ggggcgagtt cgcggatgtg
    19861 tgtgagcgcc tcgaagccgt tcctgctttc gccgaccact tcgatgtccg gctcttcggc
    19921 gagcaggcgg cgcacgcgtg cacgtgcgac gggttcgtcg tcgacaatca atgcgctggt
    19981 tttcctgagc acgctttccg ccaccgcccg cgtccctcga ccgcctttgt tagttcgccg
    20041 cttccgagcg ataaggaatt actaaagtga cttccgaccc gccgccctga acgtcgcgca
    20101 gttcgagacg gtgcttcccg tcgtagagct gttcgagcct gcggcgcgtg ttgcgcaggc
    20161 cgacgccttc cctaaggccg ttcgtccgcc agcctgcggg cagcccggtg ccgagcgctc
    20221 gccgcccaaa gagacgctga tgcgtgtggc tcgcgcgacg acgtgctcat tggtcaggat
    20281 gtgcccctgc ttgtcgatga tcgagccgct gcccgtgccg ctgcgcccgc cgtaatccca
    20341 caggttgcgc ccgcccgccg aagaggatgt gatgaagacg acccccggcg agagggcgcg
    20401 gtacacatcg atgttgttct gctcgtcggt cgccgaactc ggatcggcga tgcccggcgt
    20461 tggcgcactt tcggctgcgg cgctggcgac gtaaggcggc ctgtcgatga agttgatgcc
    20521 cgttaccacc agcgccgaga acgtcgcgga gagcagcacg agcgagaaaa tttgtcggag
    20581 cgaaagtttg tatctcataa ttgcgacccc ttgatccgtg tgttgaaaac ccgatccgct
    20641 tcctcaagcc ctgtcgcact gtagcggtgg cgcgagcgtc aggagccgtc aaagcgaaaa
    20701 atccgtacct catcggcgcg cgcctcggga cgataacttt ttgtcaaagt gggggcgcgt
    20761 ccggaggttg ccccccaacc tcgccggtcg cgcccccatc ccggacgccc cgggtgttgc
    20821 tgtccctttt gtcaatggcg tcgctgattt ggacgatgca ccggagggag gagatgcacg
    20881 aggctcggac ttatgtgacg ggtggtaggg cggcagggac gaacggtcgg cacgccagaa
    20941 acgacactgc cccggttcgt tattcgatgg tgttgcaagg ctcccctgct cccgacatgc
    21001 ttgcagcctt gatgctccta ccactttcga ctccctttgc cgcgctcggg tcacggatct
    21061 cacctcaact tgcgttagaa tgccggggct tgtgaggcgc ggcgcggtga gtgaacgtgt
    21121 cctcgcgcct tttatgtctg atgtcgttaa atgacagttc ccgagacaac atccgccgaa
    21181 gccttctggc gctacgccgg agggacgtgc ctgttcgaga gtcgggggga ggcgtggcgg
    21241 gatgtcaagg cgtggctcac cgcccgctac cacatcgact tgcggatgaa tgaagcccga
    21301 cggttgctgc gcgagacgaa gatgggtgta gtggatgtcg cactcgaagt cggctacgcc
    21361 aaccccagcc atttcgcgcg cctcttccgc agagaaaccg gcctctcccc gagcaactac
    21421 cgacggcagc gctaaccccc gccggggatt tcggggcgaa cattccaccc ctccggcaca
    21481 aaaattccac cttacaagta cggaaccgta cgtgtttccg cgccgattcg cggcaggatt
    21541 gttccctgcg ccccgtatca gatttcgagc aatatcggca caggtgaagc aagagtgcgt
    21601 gcgacggcaa acctcactcc gactacactc ctttcagcat caatcaggcg ggttaggaga
    21661 caggactatc ggaggtaggt cattatgcga gcgacagtca tgcacggcgc cggccacgtc
    21721 gatctgtacg accgggtgca gttgctcccc ttcgacaagt tggaggcttt cttcaagacg
    21781 aatttgaaat gacgcagacg ctcgacatgg cagcgaactc gcgccgagag cgtctctcgc
    21841 gagagttgaa ttggcgttcg ccgccggtgc cgggcgttag gcggccatga cacgatgagg
    21901 caaacaacaa aagcagaacg ggcgatttga gaggaggaga cgaatgcgtg gagtagtgat
    21961 gtacaccgct ggcgacgtgc gtgtggagga gcgcgaagac ccgaagattg tcgatcccac
    22021 cgacgcggtc atctggctgt gttcagatgc gtcttccttc gtcgtcggac acgccctggt
    22081 gattgatggc ggacagacgg tggcataact gccgaactaa aatggggaaa caatgatctg
    22141 gtcgagtggc tggtcaacga ccggatcgat gacgcgtatc gggagaaata cgagggccgc
    22201 ccgtacctag tcttgatgat cggcgcgcgc acgctcggcg accgtcaaga ttacggacaa
    22261 gatcgaatcg cggttcgtct cgcccgccga tttttcgcct ttgaagtaat gcgcgtagcc
    22321 ggaatgataa atattgaagc agccctgcca ttgatttatc tgagtattaa aaggaatgaa
    22381 agagatgaag cacaaagcaa ttctgttggt ggtcacggct ctggtcttgt ccgctgcctt
    22441 cgcgagccgg gcacaggaca cacaggcagg caaaccatcc cagaccatac ttcgtgccgg
    22501 ctcgcagcct tcgggcaaag gccccgccga gaatttcacc ggcaacgtca ccgtcgcccc
    22561 tctgtttccg gctgacgctt ccgcgtccta ctccggcgcg tatgtcacct tcgaggcggg
    22621 ggccaggtcg gcatggcaca tgcaccccgc agggcagcgt ctcgttgtga ccgccggggc
    22681 aggtcgcacc gcggagtggg gaggcagggt tcaggagatc aaggcgggcg acgttatctt
    22741 gtgcccgccc ggggtcaagc actggcacgg ggcatctccc aacaccgcca tgacgcacct
    22801 cgccctcacg aatagcgtca acaacaaaaa cgtcgagtgg atggagaaag tcaccgacgc
    22861 gcaatacaac gccggggtac gacctatgaa caccacacat gctctgagcg cgaagcagcg
    22921 aagcatcatc cccatcgccg ctttcaccgc caccggggat caggcgaagc tgagaacggc
    22981 tttgaacgaa ggactggagg cgggtctgac cgtgaacgag atcaaggaaa tcctggtcca
    23041 gatgtatgcg tatgccgggt tcccgcgcag cctgagcggc atccagacct tcatggccgt
    23101 gatggacgag cggcaggcca aaggcatcaa ggatcaggtg ggcagagacg ccgcccccct
    23161 gcctaccggc atgaacaggg atgagtacgg cgcgaaagtg cgggcaaagc ttgccgggca
    23221 ggaagtgatc ccgcctcccg ccggttatca actgttcacg cccgtcatcg acacgttcct
    23281 gaaagagcac ctgttcgcgg acatcttcgc ccgcgacatc ctcgatcacc ggagccggga
    23341 actcgcgacc gtcgcggcgc tctccagcat gacgggcacg cgcagtcaac ttcagttcca
    23401 cctcggcgcc gcgatgaacg tcgggatcac cgaagctcaa ttgcgggact tcatctccgt
    23461 gctcgcggcc aaggtcggcg aaagggaatc cgaaagcgcc ggagaggtgc tgagcgccgt
    23521 cctgacgacc ttaaagtgag ggggaggggc agctctaacg acaggtagtt gttcgcgcag
    23581 gtcctgcttg gcacagtttt gggtaggcgg cctttgcggg cgggagaatg gattagacat
    23641 aggcacaaac agggagagcc aaacatgaaa gacgacttgg acgaaacctc gggcactgag
    23701 ttaacggctg acgaggaggc gttgttaaag gagatgccga cgttgaccga tgtgggtcag
    23761 ggcaggcgca ccttcatggg tcagacgctc gcgggcggcc tcggcacctt cgcgctgatg
    23821 ctgctggaga aggagcgggc gctcgccgcg ctctctactt cgcccgacgc catgttcgca
    23881 cccgtaccgg cgttggagaa cccggtcagg gtcgtgctca ggatcaacgg cgcgacgaga
    23941 accctcgaac tcgattcgcg cgtggtactg ctcgacgcgt tgcgcgagcg gctcgaacta
    24001 acggggacga agaaggggtg cgatcagggg cagtgcgggg cgtgcacggt gctcgtcgat
    24061 ggccgccgcg tgctgtcgtg cctgacgctc gccgcgcagg tggaggggcg cgaggtcacg
    24121 accgtcgagg ggcttgccaa aggtgacgaa ctgcatcccg tgcaggcggc cttcatcaag
    24181 tacgacggct tccagtgcgg ctactgcaca ccgggtcaaa tctgctcggc tgtcgggatg
    24241 ctcacggagg tgcgccacgg ggacgtgagc cacgtcacgg aggacgtgac cgtgacgacg
    24301 acccggctca cggacgacga gatacgtgag cggatgagcg gcaacatctg ccgctgcggc
    24361 gcgtacccgg ggatagtcgc ggccatcagg gaggttcatt caggccgtga gtcggcgcgg
    24421 acgtggcgct tcgtcgaaga agaagagctg gctcgcatca ggaaggagga agccaatgag
    24481 gccgtttaag tacacacgca ttagcgaccc gaacgcggcc acacgcgcgt tggcggcaaa
    24541 cccgcgtgcg aagtttctgg cgggcggaac caacatcctc gacctcatga aagaggacgt
    24601 ggaacgcccc gacgaactcg tagacctgac ccgtctaaag ctcgccgaga tcaaggcggc
    24661 gggcggcggc gtctccatcg gcgcgctcgc gaagaactcc gacaccgcca accacccgct
    24721 catccgcagg ggctacccgc tactcacgca ggccatcgtc gcgggcgcgt ccggccaact
    24781 ccgcaacatg gcaaccaacg gcgggaacct gatgcagcgc acgcgctgcc agtacttcta
    24841 cgacacgacc atgccctgca acaagcgcga gccgggcacg ggctgcggcg cgctcgaagg
    24901 cttgaaccgc atccacgcca tcttcggctg gagcgacaag tgcgtggcga cttatccggg
    24961 cgacatggca aacgcgctct acgcgctcga cgccgtggtt cgggtcaggg gcgcgcgcgg
    25021 tcgtgaacgc acgatcccgg tcggcgagtt tcaccgcctg ccgggcgaca cgccggagaa
    25081 ggataccaac ctcgcgcacg gcgaactcat cgtggcaatc gaactgccgc gttcgaactt
    25141 cgctaaaaac tcctactacc tgaaagtgcg cgaccgcgcc tcctacgcct tcgcgctcgt
    25201 cgccgtggcc gccgcgctcg aactcgatgg caggcgcgtc aggcaggcgc gcgtcgtgct
    25261 cggctcagtg gcgcataagc cctggcggag tatggaggcg gaggccgcgc tcgcgggcat
    25321 gcccgcgtcg gaggagagct ttcggcgggc cgccgaggcg gcgctcgccg gcgcgaaacc
    25381 gctcgcgcac aacgcctata aggtggaact ggggaagcgc gccgtcgtac gtgcgctgat
    25441 gcgcgcggcc aagctggctt gaaagggagg ggatgacaat gccgaggtac atcgggaaag
    25501 agatgagccg cgtggacggc gtggcgaagg tgacgggcag ggcgaagtac gcggcggaat
    25561 tccgcgcgcc gaacctcgcc tacggcttta tcgtgctggg ctccatagcc aagggtcgga
    25621 tcacgaacat cgacacgcgc gaggcggagc gcgcgccggg cgtcgtgcgc gtcttcacgc
    25681 acctgaacgc gcccaagctc gggccgaaga ccgccagcga ggactctccg ccttcgtcca
    25741 gagagccgac gccacgggag gaacgagaca agtctttccg cgcgctccag tcagacagaa
    25801 tctacttcaa catgcagccc gtcgcactcg tcgtcgccga gacgtacgag caggcgcgct
    25861 acgccgcgcg cttagtccgt gtctcctaca acgccgagaa acacacgacc gacacggaat
    25921 cggtgcgcga ccgcgggcgc ttcccgccgc agggaccgcc gcccaaaccc cgcggcaacc
    25981 ccgaggcggc catgaagagc gcgccggtga agatcgaggc cgaataccgc atccctatag
    26041 agcaccacaa ccctatggag ccgcacgctg ccgtcgccgt ctggcagggc gacaagctga
    26101 ccatcttcga taagacgcag gaggtctacg gcgtgcgcgc gcacctcgcg gcggccttcg
    26161 gcgtcccgga ggagaacgtg caggtcatct ccccttatgt gggcggcgcg ttcggctcgt
    26221 ctttaaggcc gaactactac ccgtcgctca ccgcgatggc ggcgcgcgaa ctcaagcgtc
    26281 cggtcaaggt cgtctacacg cgcacgcaga tgtacacggg gcacggctac cgcccctaca
    26341 cgatccagaa gatcgcgctc ggcgcagggc gcgacggcaa gctctcggcg atgattcacg
    26401 aggccgtgca caacacctcc accttcgagg agttttcgga cgccaccacc gcgttcacgc
    26461 ggcaggtcta cgcctgcccg aacctctctg cgccgctcag gatcaccgcg accgacctca
    26521 acacgccgac ctggatgcgc gcccccgggg ctgtcagcgg catgttcgcg ttggagtgcg
    26581 cgatggacga gctggcttat gcgctgaaga tagacccgct cgaactccgg ctcatcaact
    26641 acgccgagcg tgaccctgag agcggaaagc cgtggtcgag caaagcgcta cgcgagtgct
    26701 acagactcgg ggcggagaag ttcggctgga agaatcgcaa gatggagccg cgctcgatgc
    26761 gcgacgggcg gctcctcgtc ggctggggca cggccaccgg cgtctggggc gcaggccaga
    26821 tgcccgccac cgccagaatt actctcaagg cggacggcac ggcgcacgtc gccagcgcga
    26881 cgagcgacat cggccccggc acctacacgg tcatcaccat gatcgccgcc gagtacttag
    26941 gcctcaagcc cgagcaggtc cagttcgaac ttggcgacac gaagttcccg cgcgccccgg
    27001 cgcagggcgg ctcatggacg acggccagcg tcggctcggc cgtctacggc gccgccctcg
    27061 cgatcgccgc gaggctgctt accctcgcga accgggacgc tgattcgccg ctgaaagcag
    27121 cagccggatc cgacgtggaa atgctcgacg gacggttaag attgaaaagc gatccgtcga
    27181 ggttcatcaa cgtctccgag gtcatgcggc gcaacaatct cgcggagatc gcggagacgt
    27241 tcgcgtcgcg cccctcgaag gagcgcgaga agtacgggct acttgcgcac ggcgcacagt
    27301 tcatcgaggt caaggtcgat cccgatctgg gtcaggtgcg cgtcacgcgc gccatcgagg
    27361 tgacggcctg cggcaagatc atcaacccta aggcgtcgca cagccaggag atcggcggag
    27421 tggtctgggg tatcggcatg gccttgcagg aggcgaccga gatcgaccac cgctacgggc
    27481 gcatcatgaa cccgaacctc cagcactacc acgtccccgt taacgccgac gtgcacgagg
    27541 tggagacgat gttcgtcgaa gaggaggaca aggtggtcaa cccgctcggc gtcaagggca
    27601 tgggcgaact cgggatggtc ggcatcccgg cggccatcgc caacgccgtc ttccatgcca
    27661 caggcaaacg catcagagaa ctgcccatca cgccggacaa gttgctgtag agagaggtga
    27721 actgatgaac gaagtgcgag cgttagtcga agcgttcgac ggggcgaacg agaggggcga
    27781 gcggtgcgcg ctggcgacgg tcgtgagcgt cgaaggctct tcgtaccggc
//
LOCUS       DASWNS010000494        15955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_148847, whole genome shotgun sequence.
ACCESSION   DASWNS010000494 DASWNS010000000
VERSION     DASWNS010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148847"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..658
                     /locus_tag="VGB76_17015"
     CDS             278..658
                     /locus_tag="VGB76_17015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0100650.1"
                     /translation="MPNILIVDDSDIWRRLAKDVLKRNDYRLLEADSGEQALEIARET
                     PLDLVVMDYRMNRLNGLDTSRHLREISGYERVPVILITSENFPGDCEQTPAPYVNGYI
                     DKKHLIHELGDCVKLHLERDLSVA"
     gene            1019..1864
                     /locus_tag="VGB76_17020"
     CDS             1019..1864
                     /locus_tag="VGB76_17020"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100651.1"
                     /translation="MNRYSSISVCLLLLLLFPAAACKKSKTETAANLSAPTASPVSNV
                     EAQLAEQVRLDQEAVRQSPNESANYYNLANTYFTSGKYTEAIEQYQHAIRLDPKDASA
                     RYELGQAYLRLKRYDEAFAAFNESLKINPKNAAVYHEMGNAYVQQNRTNDALAAYQKA
                     VSLDPKHAEAHFNLGNLYNQLEKMPEAVKAFQQVTRLNPKDSDAFFNLGNSYSRLKRF
                     DEAIDAYKETVKLKPDDAQAHLRLAVLYLEKGNREAATAEYNTLKGMNPELASLLNER
                     MNQQK"
     gene            2396..3223
                     /locus_tag="VGB76_17025"
     CDS             2396..3223
                     /locus_tag="VGB76_17025"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100652.1"
                     /translation="MKNPFIASLLFFLALGGSGCSKQQKATANANTNVAPEATQAPVV
                     ANTNAEGESASGVQSPQPATPDAQGYYALGIELYKQNRDEQAVEAFRQATTLDPDFAE
                     GFMKLGLAYRALGKKEEANKAYEQAVKGFEKLLDREPKNAEAQISLGEAYSRLGDYQK
                     SIEAYRRGVRLKEPEDSATYYDIGLAYNKLARYTEAVGAFKKSIELDPDDYRAQEALE
                     RAQADASRQRARIEEEKKKLERAGKTSSNSKSNANNANNSNAKSPASSNKNQSNRNQ"
     gene            complement(3271..4044)
                     /locus_tag="VGB76_17030"
     CDS             complement(3271..4044)
                     /locus_tag="VGB76_17030"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0100653.1"
                     /translation="MDVELKERVALVTGASSGIGRATALKFAEAGARVSLAGRDEEAL
                     AWVAGEIESRGGAAYVVVADVTREADAERAVAETIERFESLDILVNAAGVLSSGTIEN
                     TTLGAWDAMLDVNLRAVFHLMQLAIPHLERRQGNIVNVSSVTGLRSFPGVLAYCVSKA
                     GVDQLTRCAALELAAKKIRVNAVNPGVVRTQVHRRGGMNEEAYAAFLEHSRGTHPLGR
                     VGEAEEVAELILFLASERAAWITGATYSIDGGRAQTCAR"
     gene            complement(4136..4885)
                     /locus_tag="VGB76_17035"
     CDS             complement(4136..4885)
                     /locus_tag="VGB76_17035"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0100654.1"
                     /translation="MKRSERRVIKAGCCGFRLSQSEYVKRFPVVEVQQTFYQPPQVKT
                     LERWRAEAPPDFEFTLKAWQLITHEARSPTYRRLKRDLTGAERAEAGSFRPTAIVQTA
                     WEVTLACAEALQASRLLFQCPSSFTPTREHVRDLRAFFERIERPRNLSCLWEPRGEWP
                     EHLVVELCRELDLTHVVDPFAARTLTPERCYYRLHGRTGWRYQYEDDELSELITMLPE
                     GGVSYVLFNNVRMLDDAARFQTLAREREAEE"
     gene            complement(4914..6599)
                     /gene="ettA"
                     /locus_tag="VGB76_17040"
     CDS             complement(4914..6599)
                     /gene="ettA"
                     /locus_tag="VGB76_17040"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY0100655.1"
                     /translation="MSSVPNKVIYSMIGVSKFYDKKPVLKDIYLSYFYGAKIGVIGLN
                     GSGKSSLLRILAGTDKEFIGETVISPGHTVGFLEQEPRLDETKTVREIVEQGVQATVD
                     LLKEYEEINERFGEEMSDDEMNKLLERQGAVQEKLDALDAWDLDSRLELAMDALRCPP
                     PETPVKILSGGEKRRVALCRLLLQQPDILLLDEPTNHLDAESVSWLEQHLQKYPGTVI
                     AVTHDRYFLDNVAGWILELDRGQGIPWQGNYSSWLEQKGQRLAQEEKQESERQKTLQR
                     ELEWIRMSPKGRQAKSKARINSYEALASQETQTRASQLEIYIPPGPRLGDIVIQAEHV
                     SKAFGDRLLIDDLTFSLPPGGIVGIIGPNGAGKTTLFRMITKQEEPDNGSFRTGETVK
                     LAYVDQSRDALAPDKTIWEEIADGLDIVMLGTRQVNSRAYVARFNFSGGDQQKKVGAL
                     SGGERNRVHLAKMLKQEANVLLLDEPTNDLDVNTMRALEEALENFAGCAVVISHDRWF
                     LDRIATHILAFEGESKVYWFEGNYSEYEADRRARFGAAADQPHRIKYRQLTRG"
     gene            complement(6688..7410)
                     /locus_tag="VGB76_17045"
     CDS             complement(6688..7410)
                     /locus_tag="VGB76_17045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0100656.1"
                     /translation="MKKLLNSSLALCAILILAAQSPAQKRTPRPAQKPAATSTPAPTP
                     SASPSPTPQPTPAAEPFDAATVEQMAAQCVRLETEAGTIEMKMLPEVAPETVRNFLNL
                     VATGAYDTTTFSRVVKNFVIQGGNLSTRENLTPALAVRSRRTIPDEPGQVKHVRGVVS
                     MARPDRPNAATTHFFILVSEAASLNGTFAAFGRVTKGMDVVDAINKVAVEGETPLTPV
                     RVGSAVVFACPASPASPASPAQ"
     gene            7516..8031
                     /locus_tag="VGB76_17050"
     CDS             7516..8031
                     /locus_tag="VGB76_17050"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0100657.1"
                     /translation="MKRTGRARRTRNVEHRLMEELSLQERYAPQSACFGCGPANEQGL
                     RIRSFPHGDEVVCEWQPQAQHEAYPGMLNGGIIGSLIDCHANWTAAWHLMRRAGTDSP
                     PCTVTAEYSVKLLRPTPTDSFVTINARLIEATDNRAIVEAELVAGGKVCATGRGTFVA
                     VKPGHPAYHRW"
     gene            complement(8087..8893)
                     /locus_tag="VGB76_17055"
     CDS             complement(8087..8893)
                     /locus_tag="VGB76_17055"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HEY0100658.1"
                     /translation="MTEKSPRRAKAALLIFILSGTLCFASCSAPSESAPAAPPTSNTK
                     PSAELLQQADQSYADREDLARVREGLELLRRARAVDYSNFDVTWRTARLNYTLGDKTT
                     DATEREKAFQQGIEAGETAVRAEPERVEGHFWLGANQGGYAQARGPLYGLAAAEGLRK
                     EMETVLKLDEGFQGGSAFMVLGRLDLELPSMLGGDPKRAIETLERGMKYGEQNALYRL
                     RLAEAYVRNKRMNEARQQINFILNMTPHPAFIPEHKEAAAKARELLEKRF"
     gene            complement(9001..11637)
                     /locus_tag="VGB76_17060"
     CDS             complement(9001..11637)
                     /locus_tag="VGB76_17060"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0100659.1"
                     /translation="MSEFINYGIDLGTTNSCIVRLDAGEFKIIQNADQMNVTPSVVRV
                     LRSGSVMVGRRAYNAIAEDPDNVAAEFKRLMGQKFQQEFPASGRSMSPEELSAEVLKA
                     MLGDARRRQTNAHDLSNAAVITVPAAFTALQCEATARAAALAGLAEYPLLQEPIAAAI
                     AYGARPESQDQRWLVFDLGGGTLDIAVVSTREGRLSVLEHRGDNHLGGKDIDRLIVQN
                     IFLPYLATQFALPGAQADGRLLRKLSLIAEYAKIELSTLDETIVSLIDLGRDAQGAFI
                     EAELPLRREQIETEIEPLVARCLQLAADALAGARVKGSDLDRVLLVGGPTQMPFVRRL
                     INERLGAQVDSSLDPMTVVAYGAAIYGSTIERAASSAQVAVATEKLRLRLAFDPVSAS
                     TEAAVAGKLDGARANEAREVKIDTDNNFWTSGWHAISDNYFEMPLVLQEGKNNNFQIS
                     IRDGQGRALEVEPAEFSVRHGLMISAPPLPHTISVELVQADGTIELSPIFPRRTLLPA
                     EKTVSYRAAKTLRPSDAETSLAIKLWEGEALSEPQANNWVGNMHIWNDHVRRPIPEGA
                     EIQLHIRIDASRLITVEVFVPHLNANFSERVYIPQDEEPDYLQLLESVPLELESYAFR
                     LTALEDQLLMGDTTTGEESFDFPEVLYEEGETPASPGFVSEGETESPVESSDNSPQGE
                     IDRMRREIEDLDIEIIIRRSTGASLDADAAKGIVERAREIRVRLANLEKRFGGEQKSS
                     RVLEAESLTSETKKYVDDYGNESQKARFSALAREMERATARRDQRGIRKVNNALKSLR
                     GQILYAQEWFWREWFDYFQKPGRSFVNPEEADKWLAQGADALAQDDRAKLEEAVRRLW
                     RLEPVEETAANKERSMRPGLKL"
     gene            complement(11671..12042)
                     /locus_tag="VGB76_17065"
     CDS             complement(11671..12042)
                     /locus_tag="VGB76_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100660.1"
                     /translation="MASQELPDQLPRKLDVPLDSLVELAIESWRLEQWLAGLPPEKGA
                     SKLRHIARRLQKFLTERELAALDVTGQKYEPGMAVEVLEVLTGENLRAEEKLIVEMVS
                     PIILWRGRVVRHGQIVVGQGI"
     gene            complement(12103..13899)
                     /locus_tag="VGB76_17070"
     CDS             complement(12103..13899)
                     /locus_tag="VGB76_17070"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0100661.1"
                     /translation="MQTTTSDLLRTTQSASPGERKLMPLAANAFRLLGLSCHATQKEV
                     YAAAASIRRACKLGVTQTPAPHVAWLGSLERTENSVRDALSRLTDPAQRIHERFYWFF
                     DPQHIPAEPGLPALQESIARLRAAPQPSTGHDIALASLAVMLRLDPELCLTGEWQRTY
                     ALWKELIEAKEFWSLLVAADLKGDFEQVTTFAEVRNLRGRAWRLVTAPVAEIAQDGIL
                     RTDHSLTRRALAILRRSELPQTLADEYENQILAPVEDEFDALFAEAFRVYRYYVKSNQ
                     SVAERRATCQRTLARFEEEVKPALKKVFELAGAQSVVARRVFEVAAEGLDELADGFES
                     AFEPASRLKMLRSAWQYAPPESATLLLIEEHLTAAGDLREREPKTDSDYAHQVRRALL
                     SPPVKQPELFINYIQKEKAAKTFEGRGARVLKVALLLLLALFVGRCFYKLPGSRRTGA
                     LPPLNFNAGVLRSSPTPPPELQPDNHVFHTPPSMVEVGMILKTAVVVDVRPKNAYDAE
                     HIEGAISIPAGELAARAGRLPKDKHIIFYGGDMESARRAALELQALGFKDASVLEGGY
                     RAWLDARLPVKRQKASAGKGDRPVAGGNRREP"
     gene            complement(14146..15249)
                     /gene="ada"
                     /locus_tag="VGB76_17075"
     CDS             complement(14146..15249)
                     /gene="ada"
                     /locus_tag="VGB76_17075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEY0100662.1"
                     /translation="MGQEETLFDELRTIEEETRWRAVETRDASFNGIFFYGVRSTGVY
                     CKPSCPSRRPRRAQVVFYASADEAEAEKFRACRRCRPREVGARDARLDMVEQACRLIE
                     AATPGEGWPSLNELGQRLGVSSYHLQREFKKIVGVSPRQYGVAHRIKQFKSRIKKGES
                     VIDAMNEAGYGSSSRLYEHTNAELGMTPASYARGGAGAHITFTIAPCELGRILVAATP
                     RGLCAVRLGDTEAELEATLRAEFSQAELSRDAHALGEYVEAILRHLAGQQTSLELPLD
                     VRATAFQRRVWEELRRIPFGSTRSYREVAESLGQPTAVRAVAGACAANPVALVTPCHR
                     VVREDGSLSGYRWGTERKAKLLAQEQTLASKRT"
BASE COUNT         3102 a         4995 c         4569 g         3289 t
ORIGIN      
        1 atgcggctga tctggctgat cttgagcggc gtgcgttgca actggtgctg cgtgccttcg
       61 ccgcaatcga acagccacac ttcgccgcgc tgcggcaggc gcaaggccac gctcgaaaca
      121 ttgcgcgctc tcgtcggcac gcccgaactc gtccctaaaa aagtgagttg caacgccggt
      181 tcacgactcc cgtttatagt atttgactta aaactgaata tggcttaaga caattattga
      241 ttttgcctga ccccgagcgc aaaaggattt tcccccaatg ccaaacattt tgattgtaga
      301 tgactcggac atctggcgtc gcctcgccaa agatgttctc aagcgcaacg actaccggct
      361 gctcgaagca gactccggcg agcaagctct ggagatcgca cgcgagactc cgctcgacct
      421 cgttgtcatg gattatcgta tgaaccgtct gaacggtctg gacacctcgc gccacctgcg
      481 cgagatctcc ggctacgagc gcgtccccgt tattctcatc acgtccgaga attttcccgg
      541 cgactgtgaa cagacgcccg cgccctacgt gaacggctac atcgacaaga aacatttaat
      601 ccacgagttg ggcgattgcg tcaaactgca tctcgaacgc gacctgagcg tggcttaaat
      661 tcgagccgtg ggttaaatgt tcgcgtttcg ttcgattttt gcgcgcaact acttgtcatg
      721 cgcgcgtttt ggtgttagag tccgcagcca tatcgcatct ttcccccaaa aaaggaacgg
      781 tgtgagagac aaaaggtcgt ctgcgcagcg agggcgcaga cgaccttttg ccatgtcaga
      841 cgctcgatca aatctccgtc gtctcccttt ctctcgcacc gcattttaaa gtttcaacgc
      901 cgcgccgttt tccgtctcga ctcggacgct caagtttgcg cgcgaccgct tatcgttgtt
      961 aatcttatca gccgtgccat ttgcagcatc aactcttaat tcagcgcgag cgaataaaat
     1021 gaatagatac tcgtccatct ccgtctgctt acttttactg cttctcttcc ccgcggcggc
     1081 ctgcaaaaaa tcaaagactg aaaccgccgc caatctctcc gcgccgaccg cttcgcccgt
     1141 ctcaaatgtc gaagcgcagt tagccgaaca ggtcaggctc gatcaggaag ccgtgcgtca
     1201 aagtccgaac gagtcggcaa attattacaa tctcgctaat acttatttca cctccgggaa
     1261 atacacggag gccatcgagc aataccagca cgccattcgc ctcgacccga aagacgcctc
     1321 cgctcgttac gaactggggc aggcttacct gcgtctgaaa cgctacgacg aagcgtttgc
     1381 cgctttcaac gagtctctta aaatcaatcc taaaaacgca gccgtctatc acgagatggg
     1441 caatgcctac gtccaacaga atcggactaa tgacgcgctc gccgcttatc aaaaggcagt
     1501 cagtctcgac cccaagcacg ccgaggcgca tttcaatctc ggcaatctct acaaccagtt
     1561 ggagaaaatg ccggaggccg tcaaagcatt ccagcaggtg acccggctca acccgaaaga
     1621 ctctgacgct tttttcaatc tcggcaactc atacagtcgc ttgaagcgtt tcgacgaagc
     1681 aattgacgcc tacaaggaga ctgttaagct caagccggac gacgcacagg cgcacctgcg
     1741 tctcgccgtt ttgtatctgg aaaaaggtaa tcgcgaggcg gccaccgccg aatacaacac
     1801 tttgaaaggc atgaacccgg aactcgcctc gctcttaaac gagcgtatga accagcaaaa
     1861 gtgagcgcgc gaaaataaat ttcggtttat tcgttatgta atccaaccgc aatcactttg
     1921 caacctgtgg gcggcggccc tcccgtatag tccgctcatc cttttacggc aactggacgg
     1981 acaaaatgga gcggcgggct tgggggagtc agccgctcca ttctcctttt atacgaaccc
     2041 atcccgcgtt ctccgctttc gcccacacac tattcgagta cgattgcgta cagacgctcc
     2101 catttttgag agactagaat ccacgccctt cgcacgtcga aaatctgtca taggaagtag
     2161 tctcgaaact gctgacctat gaagcaaagc gcacggctga agtaacattc aaacaaaact
     2221 tggggcaggg tcgctcttcc taaagaacgc gctctctgcc catttcttct gcggcggcaa
     2281 acctgcgccg ggcgtcatcc tctcattaaa tttccctgac gatgcggttg actcgtgctg
     2341 cgatttagct tacataattc tagctccccg gcatcaaaca gcgcaggttt cggttatgaa
     2401 aaatcccttc atcgcgtcgc tcctcttttt tcttgccctc ggcgggtcgg gttgctctaa
     2461 acaacaaaag gccacggcga acgcaaacac aaacgtcgct cccgaagcta ctcaagctcc
     2521 cgtcgtcgcc aacaccaacg cggagggcga gtcagcttcc ggcgtccaaa gcccgcagcc
     2581 cgccacccct gacgcgcaag gctattacgc gctcggcatc gaactctaca agcagaatcg
     2641 tgacgaacag gcggtggaag ccttccggca agcgactacg ctcgaccccg atttcgccga
     2701 aggcttcatg aaactcgggc tggcttacag ggcgctcggc aagaaggagg aggccaacaa
     2761 agcatacgag caggccgtca aaggctttga aaaattgctc gaccgcgagc cgaagaacgc
     2821 ggaggcgcaa atcagtctgg gcgaggctta cagtcgtctc ggcgactacc agaaatccat
     2881 agaggcttat cgtcgcggcg tgcgcctcaa ggaacccgag gacagtgcga cctattacga
     2941 catcgggctg gcctacaata aactcgcgcg ctacacggaa gcggtcggcg cgtttaaaaa
     3001 gtctatcgaa ctcgacccgg acgactaccg cgcgcaggaa gctctggagc gcgcgcaggc
     3061 cgacgccagc cgccagcggg cacgcatcga agaggaaaag aagaagctgg aacgcgcggg
     3121 caagacaagt tccaactcca agagcaacgc caacaacgcc aacaactcga acgccaagtc
     3181 cccggcgtcg agcaataaga accaatcaaa cagaaaccag taagagcgcg tcgcagaact
     3241 gctctgtttt gcgcgtgcgt aatcttcact ttaacgcgcg caggtctgcg cccgcccgcc
     3301 gtcaatcgaa taggttgcgc ccgtgatcca cgcggcgcgc tccgaggcca aaaacaggat
     3361 caactccgcg acctcctccg cttccccgac gcgccccagc gggtgcgtcc cgcgcgaatg
     3421 ttcgaggaac gccgcataag cctcctcgtt catgccgccg cgccggtgca cctgcgtccg
     3481 cacgacgccc gggttcaccg cgttgaccct gatctttttg gccgccaact ccagcgcggc
     3541 gcaacgcgtc aactgatcaa ctccggcctt cgagacgcaa taggcgagga cgccgggaaa
     3601 cgagcgcaag cccgtcacgc tcgaaacgtt cacgatgttt ccctgtcgcc gctcaagatg
     3661 cggaatcgcc agttgcatca aatgaaagac ggcgcgcagg ttcacatcca gcatcgcatc
     3721 ccacgctccc agcgtcgtgt tctcaatcgt tccgctcgac aacacgcccg ccgcgttgac
     3781 gagtatatcc agactctcga aacgctcgat tgtctcggcc acggcgcgct ccgcatccgc
     3841 ctcccgcgtg acatccgcaa cgacgacgta tgctgcgcct ccgcgactct caatctcgcc
     3901 cgccacccat gccagagctt cctcatcgcg cccggcgagc gacacgcgcg cgcccgcttc
     3961 ggcaaacttc aaagcggtcg cccgcccgat gcccgaactc gcacccgtca cgagcgcgac
     4021 cctctctttc aattcaacat ccatcttcca gtctcaaccc ttcatccttc gattgtttcc
     4081 agacacttcg tcccgcgcac attcctaaac ttccgcccga ctgattctca cgcctctact
     4141 cctccgcctc ccgctcgcgc gccagcgttt gaaatcgcgc cgcatcgtcc agcatccgca
     4201 cgttgttgaa gagcacatac gagacgccgc cctccggcag catcgtgatg agttccgaga
     4261 gttcgtcgtc ttcgtactgg taacgccagc cggtgcgacc gtgcaggcga taataacaac
     4321 gctccggcgt cagcgtgcgc gcggcaaagg gatcgacgac gtgtgtgagg tcgagttcgc
     4381 ggcatagctc tacgaccaga tgttccggcc actcaccgcg cggctcccag agacaggaga
     4441 gatttcgcgg tcgttcgatg cgctcgaaaa acgcgcgcaa gtcgcgcaca tgctcgcgcg
     4501 tcggcgtgaa gctcgaagga cattgaaaca ggaggcgcga ggcttgcagg gcttcggcgc
     4561 aggcgagcgt cacctcccag gctgtctgca caatcgccgt cggtcgaaac gaacctgcct
     4621 ccgccctttc cgcgccggtc aaatctcttt tcagacgccg gtaggtcggg ctgcgcgcct
     4681 cgtgcgtgat gagttgccac gccttcaacg tgaattcaaa atcgggcgga gcctccgcgc
     4741 gccagcgttc cagcgtcttc acctgcggcg gctggtagaa cgtctgctgc acttctacaa
     4801 ccgggaagcg tttcacatac tcggactgcg acagccgaaa cccgcagcag cccgctttga
     4861 tcacacgcct ctcgcttctc ttcacctgat ttgacgctta actttccccg actctagccg
     4921 cgcgtcaact gccgatactt gatgcggtgc ggttgatcgg cagccgcgcc gaaacgcgcg
     4981 cgacggtcgg cttcgtattc cgaatagttg ccctcgaacc agtagacttt gctctcgccc
     5041 tcgaaggcga ggatgtgcgt ggcgatgcgg tcgagaaacc agcggtcgtg gctgatgacg
     5101 acggcgcaac cggcgaagtt ttccaaagcc tcttccagag cgcgcatcgt gttcacgtcg
     5161 agatcgttcg tcggctcgtc gagcaggagc acgttcgcct cctgcttcaa catcttcgcc
     5221 agatgcacgc gattgcgttc gccgccagag agcgcgccca ccttcttctg ctggtcgccg
     5281 ccggagaaat tgaagcgcgc cacgtaagcg cgcgagttca cctgccgcgt gccgagcatc
     5341 acgatgtcca gaccgtccgc gatctcttcc cagatcgtct tgtcgggcgc gagcgcgtcg
     5401 cggctctggt ctacgtaggc gagtttgacc gtctcgcccg tgcgaaaact cccgttgtcc
     5461 ggttcctcct gtttcgtgat catgcgaaag agcgtggtct tccccgcgcc gttcggtccg
     5521 atgatgccga cgatgccgcc cggcggcagc gaaaacgtca ggtcgtctat caacagacga
     5581 tccccgaagg ctttcgagac gtgttcggcc tgaatcacga tgtcgcccag acgtggaccc
     5641 ggcgggatgt aaatctccaa ctgcgaagcg cgcgtctgcg tctcctgact cgccagagcc
     5701 tcgtaagagt tgatgcgcgc tttcgatttc gcctgacggc ctttgggcga catcctgatc
     5761 cattccaact cgcgctgcaa agtcttctgc cgttccgact cctgcttctc ttcctgcgcg
     5821 agccgctgcc ccttctgttc gagccacgac gaataattgc cctgccacgg aatgccctgt
     5881 ccacggtcga gttcgagaat ccaaccggcg acgttgtcca gaaagtagcg gtcgtgcgtg
     5941 acggcgatga ccgtgccggg atacttctgc aagtgttgtt cgagccaact gacggattct
     6001 gcgtcgagat ggttggtcgg ttcgtcgagg agcagaatgt cgggttgctg caagagcagg
     6061 cggcagagcg cgacgcggcg tttctctccg cccgaaagaa ttttcacggg cgtctccggc
     6121 ggcgggcagc gcagcgcgtc catcgcgagt tcgaggcgcg agtccaaatc ccatgcgtcc
     6181 aacgcgtcga gtttctcctg cactgcgccc tgtcgttcga gcagtttgtt catctcgtcg
     6241 tccgacatct cttcgccgaa ccgctcgttg atctcttcgt actctttcaa cagatcaacc
     6301 gtcgcctgca cgccctgttc gacgatctcg cgcacggtct tcgtctcatc gaggcgcggc
     6361 tcctgttcga ggaagccgac ggtgtggccg ggcgagatca ccgtctcgcc gataaactcc
     6421 ttgtctgtgc ccgcgagtat ccgcagcaaa gaactcttgc ccgaaccatt gaggccgatg
     6481 acgccgatct tcgcgccgta aaaatacgaa agataaatgt ctttgaggac gggcttctta
     6541 tcgtaaaact tgctcacgcc gatcatcgaa tagatcactt tattgggtac gctgctcata
     6601 actctctaca ccttctcctt aagtaacttt gcgaaaacag cattgcgaaa tcatgcacaa
     6661 atttttgacg ctcacgcttc tataacttta ctgcgccgga ctcgctggac tcgctggact
     6721 tgctggacag gcgaacacga ccgcgctccc gacgcgaacg ggcgtaagcg gcgtttcgcc
     6781 ttcaaccgca actttattga tggcgtccac cacgtccatg cctttcgtga cgcgcccgaa
     6841 ggccgcaaac gtgccgttca gagaggccgc ctccgagacg agaatgaaga agtgcgtcgt
     6901 cgccgcgttg ggacggtcgg ggcgcgccat cgagacgacg ccgcgcacgt gtttgacctg
     6961 tcccggctcg tccgggatcg tgcgacgcga acgcacggcg agcgcgggcg tcaggttttc
     7021 gcgcgtcgag aggttgccgc cctgtatgac gaaatttttg acgacgcggc tgaaggtcgt
     7081 cgtgtcatac gcgcctgtgg cgacgaggtt cagaaagttg cggacggttt cgggcgcaac
     7141 ctccggcagc atcttcatct caatcgtgcc cgcctccgtc tcaagcctta cgcactgcgc
     7201 ggccatctgc tcaaccgtcg cggcgtcaaa cggctctgcg gcgggtgtcg gttgtggcgt
     7261 gggcgacgga ctcgcagagg gcgtcggcgc aggcgtgctc gtcgcggcgg gtttctgtgc
     7321 gggacgcggc gtgcgttttt gtgcgggcga ttgagcagcc agaataagta tggcgcagag
     7381 cgcgaggctt gagttaagaa gttttttcat agtctcttta tgtgaatttg tgaattactg
     7441 ctatatcatt agcacaaagc gcgtcgctca tgcacgagcc ggaaagtgtg aagcaggcga
     7501 aaatcggttc cctcgatgaa gcgaacgggg cgtgcgcgcc ggacgagaaa cgtggagcac
     7561 aggttgatgg aagaattgag cttacaggag agatacgcgc cgcagtcggc ctgcttcggt
     7621 tgtggtccgg ccaacgaaca gggtttgcgc atacgaagtt ttccgcacgg cgacgaagtg
     7681 gtttgcgaat ggcaaccgca ggcgcaacac gaggcatatc ccggcatgct gaacggcggc
     7741 atcatcggct cgctcattga ttgccacgcc aactggacag ccgcgtggca tctcatgcgc
     7801 cgcgccggaa ccgactcgcc gccctgcacc gtgacggctg aatactccgt taaactcctg
     7861 cgccctacgc cgaccgattc tttcgtgacc atcaacgcac gcctcatcga agcgacggat
     7921 aatcgcgcta tcgtcgaagc cgaactcgtg gcgggcggaa aagtgtgcgc cacggggcgc
     7981 ggaacattcg tcgctgtcaa gcccggccat cccgcctatc atcgctggta aataagccgg
     8041 cctatgtagt cgctccgtac tctatgcggc gagtgccttc attcgtttag aagcgtttct
     8101 caagtagctc gcgtgctttc gcggcggctt ctttgtgttc gggaatgaaa gcggggtgcg
     8161 gcgtcatgtt gaggatgaag ttgatctgct ggcgcgcttc attcatgcgc ttgtttctca
     8221 cgtaagcctc ggccagacgg agacggtaga gcgcgttttg ctctccgtat ttcatgccgc
     8281 gctctaaagt ctcgatggcg cgcttgggat cgcccccgag catggagggg agttcgaggt
     8341 cgaggcgtcc gagcaccatg aacgcgctcc cgccttgaaa accttcgtcg agtttgagga
     8401 cggtttccat ctccttgcgc aaaccttccg ccgccgcgag gccatagaga ggtccacgcg
     8461 cctgcgcgta gccgccctga tttgcgccga gccagaagtg gccttcgacg cgctccggct
     8521 cagccctcac cgccgtctct ccggcttcga tgccttgttg aaaagccttt tcgcgctcgg
     8581 tcgcgtcggt cgttttatcg ccgagcgtgt agttgagccg cgcggtacgc cacgtcacgt
     8641 cgaaattgct gtaatcgacg gcgcgtgcgc gtcgcagcag ttccaacccc tcgcgcacgc
     8701 gcgccaggtc ttcacgatca gcataacttt ggtcggcctg ctgcaagagt tccgcgctcg
     8761 gcttggtgtt cgaggtggga ggcgcggcgg gcgcgctctc ggagggcgcg gagcaggacg
     8821 cgaaacagag cgtgcctgac aggataaaga tcaacagggc agcttttgcg cggcgaggac
     8881 tcttctctgt catcaaattt aaaatgttcc tttctttcgg ttcggcttgt acgcacgcgc
     8941 atgtgtatgc gcccggtgat gtaatccgca agcgacttcg ctccgcatgc cctcgacgcc
     9001 ttacaacttc aggccggggc gcatcgagcg ttccttattc gccgccgtct cttcgacggg
     9061 ttcgagtcgc cagagtcgcc tcacggcctc ttcgagtttc gcccgatcat cctgtgcgag
     9121 cgcatctgcc ccttgcgcga gccacttgtc ggcctcttcc gggttgacga agctgcgccc
     9181 cggcttttgg aagtagtcga accactcacg ccagaaccac tcctgcgcgt agagaatctg
     9241 cccgcgcaga ctcttcagag cgttgttgac cttcctgatt ccgcgctggt cgcggcgtgc
     9301 cgtcgcgcgt tccatctcgc gcgcgagcgc actgaaacgc gccttctgcg actcgttccc
     9361 gtaatcgtca acatacttct tcgtctccga agtgagcgac tcggcttcca gcacgcggga
     9421 actcttttgc tcgccgccga agcgtttttc gagattcgcc agccgcacgc gtatctcccg
     9481 cgcacgctcg acgatgccct tcgccgcgtc agcatcgagc gacgcgcccg tgcttctacg
     9541 gatgatgatc tcgatgtcca aatcttcgat ctcgcgtcgc atgcgatcaa tttcgccctg
     9601 cgggctgttg tcgctagact cgaccggaga ctctgtctcc ccctcggaga cgaagccggg
     9661 cgacgcgggc gtctcgcctt cttcgtagag cacttccgga aaatcgaagc tctcttcgcc
     9721 cgtcgtcgta tcgcccatga gcaattgatc ttcgagcgcg gtgaggcgaa acgcgtaaga
     9781 ctcaagttcg agcgggacgg attcgagcag ttgcagataa tcgggttctt cgtcctgcgg
     9841 gatgtagacg cgctcgctga aattcgcgtt gagatgcggg acgaaaactt ccacggtgat
     9901 caggcgcgag gcatcaatcc tgatatgcag ttgaatctcc gcgccttcgg gaatcggacg
     9961 acggacatga tcgttccaga tgtgcatgtt gccgacccag ttgttcgcct gcggctcgct
    10021 cagggcttcg ccctcccaga gtttgattgc gagagacgtt tccgcgtcgc tcggtctgag
    10081 cgtcttcgcg gcgcgatagg agacggtctt ttcggcgggc agcagcgtgc ggcgcgggaa
    10141 gatcgggctg agttcaatcg tgccgtcggc ctgtaccaac tcgacggaga tcgtgtgcgg
    10201 cagaggcggc gcgctgatca tcaggccgtg acggacggag aactccgccg gttcaacttc
    10261 gagagcgcgc ccctgtccgt cgcggatgct gatctgaaaa ttgttgttct tgccttcctg
    10321 caagacgagc ggcatctcga aatagttgtc gctgatggcg tgccaaccgc tcgtccaaaa
    10381 attgttgtcg gtatcaatct tcacttcgcg cgcctcattc gcgcgcgcac cgtcgagttt
    10441 ccccgcgacc gccgcttccg tggatgcgct caccggatcg aaggcgagac gcaggcgcag
    10501 cttttccgtc gcgacggcga cctgcgcgga agatgcggcg cgttcgatgg tcgaaccgta
    10561 gatggccgcg ccgtaggcca cgaccgtcat cgggtcgagc gaactatcaa cctgcgcgcc
    10621 gagcctctcg ttgatcagac ggcgcacgaa aggcatctgc gtgggaccgc cgacgagcag
    10681 gacgcggtcg agatcgctgc ctttgacgcg tgcgcccgcg agcgcgtccg ccgccagttg
    10741 caggcagcgc gcgacgagcg gttcgatctc cgtctcgatc tgctcgcggc gcaggggaag
    10801 ctcggcttcg atgaatgcgc cttgcgcgtc tcgcccaagg tctatcaggc tgacgatagt
    10861 ttcgtcgagc gtcgaaagtt cgatcttggc atactccgcg atgagactta atttgcgcag
    10921 gaggcgcccg tcggcttgcg cgccgggcag cgcgaactgc gtggcgagat aggggaggaa
    10981 gatgttctgc acgatgaggc ggtcgatatc tttgccgccg agatgattgt cgcccctgtg
    11041 ttcgagcacg ctcagacgcc cttctcgcgt ggagacgacg gcgatgtcga gcgtcccgcc
    11101 gccgaggtcg aagacgagcc agcgctggtc ttgactctcc gggcgcgcgc cgtaggcgat
    11161 ggcggcggca atcggctctt gcagcagagg gtattcggcc agtccggcga gcgcggcggc
    11221 acgcgccgtc gcttcgcatt gcagcgcggt gaaggcggcg ggcacggtga tgacggcggc
    11281 attcgagaga tcgtgcgcgt tcgtctgcct gcggcgcgca tcgccgagca tggccttcaa
    11341 gacttccgca gacagttcct ccggcgacat cgaacgaccg gaggccggga actcctgttg
    11401 aaacttctgc cccatgagac gcttgaattc cgccgccacg ttgtcggggt cttccgcgat
    11461 agcgttgtag gcgcgtcgcc ccaccatcac gctgcccgac ctcaacactc tcaccaccga
    11521 aggcgtgacg ttcatctggt ctgcgttctg aatgatcttg aactcgcccg catcgagccg
    11581 cacgatgcag gagttcgtcg tgccgaggtc aatgccgtag ttgataaact cgctcataag
    11641 ttacataagt tacatgcgat ccagacgcgg ttaaattccc tgcccgacaa cgatctgccc
    11701 gtgccgcacg acacgaccgc gccacaggat gatcggcgac accatctcga cgatcaactt
    11761 ttcctccgca cgcaaattct cgcccgttaa aacttcgagc acttcgaccg ccatccccgg
    11821 ctcatacttt tgccccgtca cgtcgagcgc ggccagttcg cgttcggtca gaaatttttg
    11881 caggcgacgc gcgatgtgcc gcaacttcga cgcacccttt tccggcggca atccggcgag
    11941 ccactgttcg agccgccagc tttcaatcgc cagttcgacg agcgagtcga gcgggacatc
    12001 gagtttgcgt ggaagttggt ctgggagttc ctgagaagcc atttcgatgc ctgcccgcgt
    12061 cctcgcacga gccacgaacg ctcgaacgag ttagtttaac tattaaggct cacgacgatt
    12121 gccaccggct accgggcggt cgcctttgcc tgcgcttgct ttttgacgtt tgacgggcag
    12181 ccgcgcatcg agccacgccc ggtagcctcc ttcgagcacg gacgcgtctt taaatccgag
    12241 agcttgaagt tcgagcgcgg cgcgtctggc gctctccata tcgccgccgt agaagataat
    12301 gtgtttatcc ttcggcaggc gtccggcgcg ggcggcgagt tcgcccgccg ggatcgagat
    12361 cgcgccctct atgtgctcgg catcataagc atttttcggg cggacatcca ccaccaccgc
    12421 cgtcttcaat atcattccga cctccaccat gctcggcgga gtgtgaaaga cgtggttatc
    12481 gggttgaagt tcaggcggag gcgtgggcga agatcgcagc acaccggcgt tgaagttcaa
    12541 gggcgggagc gcgcccgtgc ggcgcgagcc ggggagctta taaaagcacc tgccgacgaa
    12601 gagcgcgagg aggagtagga gcgcaacttt taagacccgc gcgccgcgcc cctcgaaggt
    12661 tttcgcagcc ttctctttct ggatgtagtt tatgaacaac tccggctgct tgacgggtgg
    12721 gctgagtagc gcgcggcgca cttgatgcgc gtagtcgctg tcggtcttcg gctctcgctc
    12781 gcgcaggtcg cccgccgccg tcaggtgctc ttcaatcaag aggagcgtcg cgctttcggg
    12841 cggcgcgtat tgccatgcgc tgcgcagcat cttcaatctc gacgccggtt caaacgcgct
    12901 ctcgaacccg tccgccaact cgtccagacc ttccgccgcg acctcgaaca cgcgccgcgc
    12961 gacgacgctt tgcgctcccg ccaactcgaa gacctttttg agcgcgggct tgacctcttc
    13021 ttcaaaacgg gcgagcgtgc gctggcacgt cgctcgacgc tctgccacgc tttgattgct
    13081 tttgacgtag taacgataga cgcgaaacgc ttcggcgaac agcgcgtcga actcgtcttc
    13141 gacgggcgcg agtatctgat tttcgtattc gtcggcgagg gtctgcggaa gctcggagcg
    13201 gcgcaggatg gcaagcgcgc gtcgcgtgag cgagtggtcg gtgcgcagga tgccgtcctg
    13261 cgcaatttcg gcgacgggcg cggtgacgag ccgccacgca cgcccgcgca aattcctgac
    13321 ctctgcaaag gtcgtcacct gctcaaagtc gcctttgaga tcggcggcta cgagcagcga
    13381 ccagaattct ttcgcttcga tcaactcttt ccagagtgca taagtgcgct gccactcgcc
    13441 cgttaagcac agttcgggat cgagccggag catgacggcg agcgaggcga gcgcgatgtc
    13501 gtggccggtc gaaggctgcg gcgcggcgcg caggcgcgcg atggactctt gcagcgcagg
    13561 gaggccgggt tcagccggta tatgctgcgg gtcgaagaac cagtagaagc gttcgtggat
    13621 tcgctgcgcc gggtctgtga gacggctcaa agcatcgcgc acgctgttct ccgtgcgttc
    13681 gagcgaaccg agccaggcga cgtgaggcgc gggcgtttgc gtgacgccca acttgcacgc
    13741 gcgccggatg ctggcagcgg cggcatagac ttccttctgc gtggcatgac aggagaggcc
    13801 gaggaggcga aaagcgttag ccgcaagcgg catcaatttt cgctcgccgg gactcgcgct
    13861 ttgagtcgtg cgcaggagat cggaagtcgt ggtctgcatt taaagactgg gcaagaactt
    13921 caaagtggat tgagaagacg acaactgccg gatacttctt ccgccggaga tgaaagaccg
    13981 cgataaaaga gcgcgcgaaa ttttaccacc ttcgcgctgc tacaggctcg aaggattcgc
    14041 tgtttgcgat cagagaaagt cccgtgtcac acacagccat gtgtggcacg ggactttgga
    14101 ctctctgcac acccatcagc ggaaagctag cgggcaggcg atcttttaag ttctcttcga
    14161 ggcgagcgtc tgctcctgcg ccagcaattt cgctttgcgc tctgtgcccc aacgatagcc
    14221 gctcaagctc ccgtcttccc tgacgactcg atgacagggc gtgacgagcg cgacggggtt
    14281 ggccgcgcat gctccggcga ccgcacgcac cgctgtcggc tggccgaggc tttcggccac
    14341 ttcccggtat gagcgcgtgc tgccgaaagg gatgcgtcga agttcctccc agacgcgccg
    14401 ctggaaggcc gtcgcgcgca cgtcgagcgg gagttccaga gaagtttgct gcccggcgag
    14461 atggcgcagg atcgcttcaa cgtattcgcc gagcgcgtgc gcgtcgcggc tcaactccgc
    14521 ttgggaaaac tccgcgcgca gcgtcgcttc aagttccgcc tccgtgtcgc cgagcctgac
    14581 ggcgcataag ccgcgcggcg tcgccgccac cagtatgcgc cccagttcgc acggcgcgat
    14641 ggtgaaagtg atgtgtgcgc ccgcgccgcc gcgcgcataa gaagcgggcg tcatgccgag
    14701 ttcggcgttc gtgtgttcgt agaggcggct gctcgaaccg tagcctgcct cgttcattgc
    14761 gtctatcaca gattctcctt ttttgattct cgacttgaac tgtttgatcc ggtgcgccac
    14821 gccgtactgg cgcgggctga cgccgacgat cttcttaaac tcgcgctgca aatgataact
    14881 gctcacaccg agcctctgcc cgagttcgtt gagcgacggc cagccttctc ccggcgtggc
    14941 ggcctcgatc agtctgcaag cctgttcgac catgtcgagc cgcgcatccc gcgcgcccac
    15001 ctcgcgcgga cggcagcggc gacacgcgcg aaacttttcc gcttcggcct cgtccgccga
    15061 agcgtagaag acgacctgcg cgcgtcgcgg tcggcgcgaa gggcaggacg gcttgcaata
    15121 gacgcccgtg gagcgcacgc cgtaaaagaa tatcccgttg aaactcgcgt cgcgcgtctc
    15181 cactgcacgc cagcgcgtct cttcctctat cgtcctcaat tcgtcaaaca gagtctcttc
    15241 ctgtcccatc gcgctcacct ccatgcacgc atcttaacgc tcccggcctg cggcatctat
    15301 ccgcttcttg cggtcaaact tttccgggtt gggaacttga acgctcacgc cgccgtcctt
    15361 ctcgcctcaa gtcgccggta aaaaaaactt tcgcaccggt gatcctcatt gactcgattt
    15421 tccgcgttag catatgtagg cgaaaattgg ccgcaaaacc tcacgcacaa accggctcaa
    15481 ctttctccgg ggcgaagaga cgcgcgcccg gcattacgaa tatttgacga aacttaattc
    15541 cgtcgtgtgg aaactttagt tgccactatc tctgcgagat gataaaagga cgcgcggcaa
    15601 tcagcggcag cgatattgcc ggacaagttg accgacatag cgacgccgac gcggcgcgcc
    15661 cttcccgtct caggtttttt ccggaggtgt gttcccacca caatgccccg gtagtgttag
    15721 aatacgaccg gccaccctca caggcgggtg gccgctgcat ttttaccgtc ggaaaattta
    15781 acgaattaac gatcctgctc gttcttgaag ggagaatgaa gagatgcccg aattgcgtgg
    15841 gacgctcgcg acgccggaag tgaaggcgga atgggagcgt gcctatgagc tataccgaga
    15901 agcccccggt gcgccttacg ataagaaact tgaccgcacc gaacgcatca gctac
//
LOCUS       DASWNS010000495        13333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_173472, whole genome shotgun sequence.
ACCESSION   DASWNS010000495 DASWNS010000000
VERSION     DASWNS010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173472"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1813
                     /locus_tag="VGB76_17080"
     CDS             131..1813
                     /locus_tag="VGB76_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100663.1"
                     /translation="MNSSLRESRVAPLNSPRAFGFARRLHVLTLLLAILLGASHGAWA
                     QKSEAQKSRARKLPSPDKIVSDYLKAVGGRKRQGTILDATYEWNVQQGADAAARGTAR
                     TYTKFPASTRTDIAIDGDESKAAANARSAWLRPAGSELRTLTDREANVAKLLSALDAS
                     RLVDYKKQDVLARTIELSEGGNNIERAYVIEFSRRNGAWLRYWFGVDSKLPLQVTDDS
                     RELNIRYKDYRATQNGVLEPHRIEIRRRDAPPLAFTLNGVRYNTGLADALFEPPSDLS
                     LNIAELLRAVASNQAQLDRRVSDYTYTRKETQREFDGKGLVKTEKVVVHEVYPVAGGG
                     SVLKLISEDGVPLSPTQLEREEKRVVEELAKLERENARRREKRERERAERERKRKDAG
                     ASGEGATTEENVGVAVFLRACEFVSPRRERFQERDAIVFDFRPRPNFKPSTRSEAMVA
                     KLAGVAWIDPVDRQVMRLEARLAESYKVGGGLFVSVRPGSAVVIEQTRLADGVWLPRY
                     SQINAAAKLFLFAGFHLDATHEYSNYKRFSTVVGDAQVDAPPEPAATPPEKP"
     gene            1906..3282
                     /gene="trmFO"
                     /locus_tag="VGB76_17085"
     CDS             1906..3282
                     /gene="trmFO"
                     /locus_tag="VGB76_17085"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEY0100664.1"
                     /translation="MDEVIVIGGGFAGDEAAWQAAQNGARVRLYEMRPVRQTPAHRTE
                     QLAEIVCSNSLKSDEPGTAPYLLKEELRRGGSLVLEVAAETRVPAGAALAVDRHRFAA
                     LITQRIEAHPNITLIREEVTRIPDDAAVTIIAAGPLCSDALAEEIVRLTGDGQLYFYD
                     AIAPIVAADSIDYTIAFRAARYDKGGDDYVNCPFDEEQYRVFYDALVEARSVPLQRFE
                     ETRWFEACLPVEELARRGYDTLRYGPLKPRGLRDPRTGREPFAVVQLRQENLMADAYN
                     LVGFQNHLRYGEQARVIRLIPGLERAEFLQYGQIHRNTYIRAPRVLAATMRMRERPEV
                     YFAGQISGVEGYVESVATGWLAGINAARQLTGKPLVEAPPKSAIGALARYVSAAETDN
                     YQPVNITFALLEPLPEADRKRVRRKRERHQLQVERALTEWNNWLHHLHTDDSGTGTPI
                     TPPLAAVV"
     gene            3198..5339
                     /locus_tag="VGB76_17090"
     CDS             3198..5339
                     /locus_tag="VGB76_17090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0100665.1"
                     /translation="MEQLAAPSSHGRLRHRNSYHAAAGSGRLRARRQPRACSAAQSKL
                     ASPSGSRRKASLFVLAAILFAFAPLWPLKAQSGRRRPEPSATPAPNTQRPRRATPARP
                     AENVPGQPATPDAPSANTITPTPTPAPDNNITRTPQPTGAATPQTPQSDESVEVDDGD
                     IVRVSSNLVPVSATVTDTRGKAITDLTVDDFELRVDGQPKPIGGLSHAETPVRMVMLF
                     DNSDSIRSSREFEKQAAIRFFKSVLRPVDQAAIYSVGTVFDLVQPLTNDVQRLVRTIE
                     RFDKPEGATKLMDAMAHAAEYLRLVPGRKVLVIVSDGSDTISDLTYDETLRRVLAADC
                     QVYAVQTGVFESANLYDLMAVRRLETFSDRTGGAVYIPKSTAELDSAFAQISADLAQQ
                     YVLSYYPTDEGRDGRFRVINLRVKTRPNLRVRARRGYYPRLSAQQLSALPEHFTSTSD
                     MQQEAASGSSQSTQADPAFTISRELAIRQARSTITLGSPAHGSKNMDAETSGDPPTRN
                     ELDVRVSPAAKPLEPKAAPKAEPKAEVKTEPPPAPQSEESPPESRLAVPDAKPSPPVT
                     APSRQASTDPNPTPTPAPTATQRTQTAAPEKGSGTTSPAATTQPSQQSTDGDATKTPL
                     NVGLLNSKALSLPKPLYPEAARRMRLEGAVRVLVSVDESGKVVSARAESGHMILRGAA
                     IEAARQARFAPVLVSGQPVPVSGFITYTFGL"
     gene            5357..6280
                     /gene="xerC"
                     /locus_tag="VGB76_17095"
     CDS             5357..6280
                     /gene="xerC"
                     /locus_tag="VGB76_17095"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY0100666.1"
                     /translation="MIEQLFEQFFEHLRYERNVSEHTLRNYRIDLGQFYDHLAPADAT
                     TGARREFDLKQIDHITIREWLSTLHAANKKKTSIARKLASLRTFFQFLLREGVVEMNP
                     AKLVSTPRLEKKLPNHLSIEEAVRFIETPDTETDLGKRDRAILEFLYGTGMRVSELVK
                     LDLRDIDFKNKMVKVTGKRRKQRIVPFGDPALHALMQYLTVRGAFLNNAPLAERDEQV
                     VFLNYQGTRITTRSVGRMVDKYITICAGIHNISPHSLRHSFATHLLDGGADLRHIQEL
                     LGHARLSTTQIYTHVSMEKLIEVYDKSHPKA"
     gene            complement(6438..6815)
                     /locus_tag="VGB76_17100"
     CDS             complement(6438..6815)
                     /locus_tag="VGB76_17100"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY0100667.1"
                     /translation="MDADRLEHFRGILTKQLRQHTEHVRDNQQAALDMIADDGVKDSV
                     DMSLQDVNQELQLRLGERESQAVADIDQALLRIEEGSYGICQSCSKPIDERRLEAVPT
                     ARYDAACQSKLEAQQGLDDISTL"
     gene            7248..7574
                     /locus_tag="VGB76_17105"
     CDS             7248..7574
                     /locus_tag="VGB76_17105"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0100668.1"
                     /translation="MRTKLTLHDMARAVNLTTFHFCHLFKAETGRSPAKYLKALRLER
                     ARELLETTFLSVKEIRALVGVNDESHFARDFRNAYGLTAQQYREQFLGLDAQKKDEAE
                     QTQSGS"
     gene            complement(7767..10205)
                     /locus_tag="VGB76_17110"
     CDS             complement(7767..10205)
                     /locus_tag="VGB76_17110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0100669.1"
                     /translation="MWTACVGSAASRRLLCGVFALVVMCVCSWSAAAQSSGVSLSGHV
                     LDQFGAAVEGANVTLRQEHRNDESTTNTDAAGAFTFQGLSTGSYRLRATRRGFALADQ
                     VIRIKAGAESNIELTLQAASLAESVVVTSSRIAGVPEVVERIPGSVEVLEREMLEISR
                     VFTSSEALRKFSGVNVRDEEGFGLRPNIGLRGLNPTRSTKVLLLEDGIPLTYAPYGDN
                     ASYYHPPIDRFESVEVLKGSGQIVYGPQTVGGVVNYITRTPPEKTSGSLTLIGGNRDY
                     FNGHLNYGGTYGNTGLLFDFTRKQGEGARDQHRSGLSDFNFKSVTAIGTRQALTTKFN
                     FYRERSQVSYSGLTEAEYAANPRGNPFFNDHFFGDRFGASATHAFVFNSNTTLSTNFY
                     GSYFRRHWWRQSSNSAQRPNRLNVDPDCRSFADLNTTCGNEGRLRKYYFFGVEPRLNV
                     RHNLFGVRSETDFGVRAHYETQERLQKNGDLPNSRDGVIAENNERRNQAYSAFVQNRL
                     LFEKFTITPGVRIEHIGFERTNRLANGGLGVSGRTSLTQVVPGIGVSFQPNAGLTVFA
                     GVHRGFAPPRTEDIINNTTGGSIELDPELSWNYELGLRAAPHRGLRFEATLFRMNYEN
                     QIVPATLAGGIGSALTNGGSTLHQGAEFTGRVDSGALLGNRHNFYLRVAYTFLPTAKY
                     TGTRFSNICGFTGVSITGNRLPYAPENLLNANIGYSHPVGIDALLEGVYVGRQFGDDL
                     NLRTSPRADGQLGPVPGYAVWNAAVNYKVEQMRSTFFVTAKNLFDRTYIVDRSRGILP
                     SSPRLVQAGLKFNF"
     gene            complement(10658..11644)
                     /locus_tag="VGB76_17115"
     CDS             complement(10658..11644)
                     /locus_tag="VGB76_17115"
                     /inference="COORDINATES: protein motif:HMM:NF044507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100670.1"
                     /translation="MKKERLAAVSIYDEDLRPLDLEKVSTYPLASRPSKVATADFAKA
                     PAVDSTLRDFLSTLPNILAVRSLREVAAQVRRARELQKPIIWGIGGHVIKTGLAPVII
                     DLMKRGYVSAIAANGSVLVHDAEIALAGSTSEDVDATLGEGAFGAAEETGRLLNDAAQ
                     AGARDRLGLGEALGRALTELNPPHAGLSLLCAAYESRLPFTAHVAIGADIAHFHPTAH
                     GASLGETSHTDFRLLAELVRRMHGGGVYLNIGSAVVLPEIFLKAVTLVRNLKHPLADF
                     TTANFDFIQSYRPLTNVVRRPTSDGAGRGYSITGHHELMIPLLAAQILCGQD"
     gene            complement(11973..12902)
                     /locus_tag="VGB76_17120"
     CDS             complement(11973..12902)
                     /locus_tag="VGB76_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100671.1"
                     /translation="MTNLRIRAFLPVIFLQLIFALTNTPHAFSQATQTAPPPAQAVAT
                     TGEKATAGDEACEQSSVVNGDAAKRPGVELLDARKRTSFTSQHASLISSPRELKTGAA
                     RSFAFDANARSSSDSFANQTNPGGQAATATYTPLTSNQKMRRAFRSAFLTPQAYALPF
                     ASAVLTEWGEEDLPHKDTDDRVADGLSRFAIKFGRRSTTVLLSNGVYASLFKQDPRYE
                     RADRTRGVAARALHAASRVFITRGDNGRHQPNYSRFAGQLSGSALSNLWEQSTPGRDR
                     IGTDATFRRFGMSFVTGALSNVIREFLPGILGR"
     gene            complement(13011..>13333)
                     /locus_tag="VGB76_17125"
     CDS             complement(13011..>13333)
                     /locus_tag="VGB76_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100672.1"
                     /translation="TGLHFADVHKLLDVLQRLVSLGNTVLVIEHNLDVIKSADFVIDL
                     GPEGGAGGGRVVACGTPEEVARVKKSHTGQALKPLLDGHANNEQKSPRNGKTVTARAT
                     KKIA"
BASE COUNT         2588 a         4051 c         4198 g         2496 t
ORIGIN      
        1 attggggcga gcggcgatct ggatcgccgc tcgcccttct tcttgcctgt gccggaagct
       61 gcaaagtaag ttaagatgag cctgtcgccg gatgtccatg cccgctcgaa tttcaattcc
      121 cagatacacg atgaacagca gtctcagaga aagccgcgtc gctccgctaa acagtccgcg
      181 cgccttcggg ttcgcgcgcc gtctccacgt gctcacgttg ctgctcgcga tcctgctcgg
      241 cgcgtcgcat ggcgcgtggg cgcagaagag cgaggcgcag aagtcgcgtg cgcgaaagtt
      301 gccgtcgccg gataagatcg tgagcgacta tctgaaagcc gtcgggggac gaaaacggca
      361 gggcacgatc cttgatgcga cttacgaatg gaacgtgcag caaggcgcag acgccgccgc
      421 gcgcggcacg gcgcgcacgt acacgaaatt tcccgcctcg acgcgcaccg acatcgccat
      481 tgacggcgac gagtcgaagg cggcggccaa cgcgcgctcc gcctggttgc gccccgccgg
      541 aagcgaattg cggacgctga ccgaccggga agccaacgtc gccaagttgc tctccgcgct
      601 cgatgcgagc cgcctcgtgg attacaaaaa acaggacgtg ctcgcgcgca ccatcgaact
      661 ctcggagggc ggcaacaaca tcgaacgcgc ctacgtcatc gaattttcga ggcgcaacgg
      721 cgcgtggctg cgttactggt tcggcgtcga tagtaagttg cccctgcaag tcacggacga
      781 ctcgcgcgaa ctcaacatcc gttacaaaga ttatcgcgcc acgcagaacg gcgtgctcga
      841 accgcaccgc atcgagatca ggcggcgcga cgccccgccg ctggccttca ccttgaacgg
      901 cgtgcgctac aacacgggac tggccgacgc gctcttcgag ccgccgagcg acctctctct
      961 gaatatcgcg gagttgctcc gcgccgtcgc aagcaatcag gcgcaactcg accgtcgcgt
     1021 gagcgattac acttacacac gcaaagagac gcagcgcgag ttcgacggca agggtctggt
     1081 caagacggag aaggtcgtcg ttcacgaagt ctacccggtg gcgggcggcg ggagcgtgct
     1141 caagttgatc agtgaagacg gcgtgccgct ctcgcccacg cagttggagc gggaagagaa
     1201 gcgcgtggtg gaagagttgg cgaagctcga acgcgagaac gccaggcgca gagagaagcg
     1261 ggaacgcgaa cgcgccgagc gtgagcgcaa gcgcaaagat gcgggcgcga gcggcgaggg
     1321 cgcgacgacg gaggagaacg tgggcgtcgc ggtctttttg cgtgcgtgcg aattcgtcag
     1381 cccgcgccgc gagcgttttc aagaacgcga cgccatcgtc ttcgacttcc gcccgcgtcc
     1441 caacttcaaa ccctccacgc gcagcgaagc gatggtcgcc aagctggcgg gcgtcgcgtg
     1501 gattgatccg gtggacagac aggtgatgcg tctggaagcg cggctcgccg aaagttacaa
     1561 agtcggcggc gggctgttcg tctcggtgcg ccccggctcg gcggtcgtca tcgaacagac
     1621 gcgccttgcc gacggcgtct ggctcccgcg ctactcgcag atcaacgccg ccgccaaact
     1681 ctttctcttc gcaggctttc acctcgacgc cacgcacgaa tacagcaact acaaacgttt
     1741 cagcacagtc gtcggcgacg cgcaggttga tgcgccgccc gaacctgctg cgacgccgcc
     1801 tgagaagcct tgagtggtct ttgcgggttc gttagaagtt tagaatgagc ggaagatttg
     1861 gagcgcgtcg gggaagtctg aataaggcgg gcggtattga aaataatgga tgaagtaata
     1921 gtcatcggcg gcgggttcgc gggagatgag gcggcgtggc aggcggcgca gaatggtgcg
     1981 cgcgtgcggc tgtacgagat gcgtcccgtg cggcagacgc ccgcgcaccg gacggaacag
     2041 ttggccgaga tcgtctgttc caactcgctg aaatccgacg agccgggcac tgcgccctac
     2101 ctgttgaagg aagagttgag gcgcggcggt tcgctcgtgc tggaagtcgc ggcggagacg
     2161 cgcgtgcctg cgggcgcggc gctggcggtt gacaggcacc gcttcgcagc gttgatcacg
     2221 cagcggatcg aggcgcatcc gaacatcacc ctcattcgtg aagaggtgac gcgcattcca
     2281 gacgacgctg ccgtcacgat catcgcggcg gggccgctct gttcagacgc gctcgcagaa
     2341 gagatcgtgc gcctgacggg cgacgggcaa ctctacttct acgatgccat cgcgcccatc
     2401 gtcgccgccg attcgataga ttacaccatc gcgtttcgcg ccgcgcgcta cgacaagggc
     2461 ggtgatgatt acgtcaactg tccctttgat gaagaacagt atcgagtctt ttacgacgcg
     2521 ctcgtcgaag ccaggagcgt gccgctgcaa cgcttcgagg agacgcgctg gttcgaggcg
     2581 tgcctgcccg tcgaggaact cgcgcggcgc ggctacgaca cgctgcgcta cggcccgttg
     2641 aagccgcgcg gcctgcgcga cccgcgcacc ggaagagagc ctttcgccgt cgtccagctc
     2701 cggcaggaaa acttgatggc cgacgcctac aacctcgtgg gcttccagaa tcatctacgt
     2761 tacggcgaac aggcgcgcgt aattcgttta attccggggc tggaacgtgc agagttcttg
     2821 cagtacgggc agattcatcg taatacttat atacgcgcgc cgcgcgtgct cgccgccacg
     2881 atgcggatgc gcgagcgtcc ggaagtatat ttcgccggac agatcagcgg cgtcgaaggt
     2941 tacgtggaat cggtggcgac gggctggctc gcgggcatca atgcggcgcg gcaactgacg
     3001 ggaaagccgc tcgtcgaagc gccgccgaag tcggccatcg gagctttggc gcgttatgtc
     3061 tccgccgccg agacggacaa ttatcaaccc gtcaacatta ccttcgcgct cctcgaaccg
     3121 ctgcccgaag cggatcgcaa acgcgtccgg cgcaagcgcg agcggcatca acttcaggtc
     3181 gaacgagcat taacagagtg gaacaactgg ctgcaccatc ttcacacgga cgactccggc
     3241 accggaactc ctatcacgcc gccgctggca gcggtcgtct gagggcgcga cgacaaccgc
     3301 gtgcgtgcag cgcggcgcag agcaagcttg cgtcgccgtc cggtagccgc cgcaaggcgt
     3361 ctctcttcgt cctcgccgcg atcctcttcg cgttcgcgcc gctgtggccg ctcaaggcgc
     3421 aatcgggtcg ccgccgcccg gagccttccg ccacgcccgc gccgaacacg cagcgaccgc
     3481 gccgcgccac gcccgcacgc cccgcagaga acgtccccgg ccaacccgcg acgcccgatg
     3541 cgccgagcgc caacactatt acgccgacgc cgacgcccgc gccggacaac aacataacgc
     3601 gcacgccgca gccgaccggt gccgccacgc cgcaaactcc gcagtctgac gagtcggtcg
     3661 aagtggacga cggcgacatc gtgcgcgtga gttcgaatct cgtgcccgtc tcggcgaccg
     3721 ttacggacac gcgcggcaaa gcgatcaccg atctcacggt tgacgatttc gagttgcgtg
     3781 ttgacgggca gccgaagccc atcggcggct tgagccacgc cgagacgccc gtgcggatgg
     3841 tgatgctgtt tgataacagc gacagtattc gttcgtcgcg tgagtttgaa aagcaggccg
     3901 cgatccgttt cttcaagagc gtgctgcgtc cggtcgatca agcggcgatc tattcggtcg
     3961 gcaccgtctt cgacctcgtg cagcctttga cgaacgacgt gcagagactc gtccgcacca
     4021 tcgaacgctt cgacaagccc gaaggcgcga ccaaactcat ggacgcaatg gcgcacgccg
     4081 ccgaatacct gcgcctcgtg cccggacgca aggtgctcgt catcgtctcg gacggctccg
     4141 acaccatcag cgatctcacc tacgacgaga cgctgcgccg cgtcctcgcc gccgactgcc
     4201 aggtctatgc cgtgcagacg ggagttttcg agagcgcgaa cctgtacgat ttgatggccg
     4261 tgcggcggct cgaaaccttt tccgaccgga cgggcggcgc ggtctatatc ccgaagagca
     4321 ccgccgaact cgattccgcc ttcgcccaaa tttcggcaga cctcgcgcaa cagtacgtcc
     4381 tgagttacta cccgaccgac gaggggcgcg acgggcgttt ccgcgtcatc aacctgcgcg
     4441 tcaagacgcg acctaacctg cgcgtgcgtg cgcggcgcgg ctactacccg cgtctcagcg
     4501 cacagcagtt gtccgcgctg cccgaacact tcacgagcac gagcgacatg cagcaggagg
     4561 ctgcgagtgg tagttcgcag tcaacgcagg ccgaccccgc cttcacgatt tcgcgcgagt
     4621 tggcgatccg tcaagcccgc tccaccatca cgctcggcag tcccgcccac ggcagcaaga
     4681 acatggacgc cgagacgagc ggcgacccac ccacgcgcaa cgagttggat gtccgcgtca
     4741 gccctgcggc gaagcctctg gagccgaaag cagcgccgaa agcagagccg aaggcggaag
     4801 tgaagacgga accgccgccc gctccgcaaa gcgaagagtc gccgcctgag tcgagactcg
     4861 ccgtgcctga cgcgaagccg tcgccgcccg tcaccgcgcc gtctcgccaa gcctcaaccg
     4921 acccgaatcc gacacccaca cccgctccga cagcaacgca gagaacacag acagccgctc
     4981 ccgaaaaggg ttcgggcacc acctcgcccg cagctacgac gcagccttcg cagcagtcaa
     5041 cggacgggga cgcgaccaag accccgctca acgtgggtct actcaactcg aaagccctga
     5101 gtctcccgaa accgctctac ccggaggcgg cgcggcgcat gcggctcgaa ggcgcggtgc
     5161 gtgtgctggt gtcggttgac gagagtggta aagttgtttc cgcacgcgcc gagagcgggc
     5221 acatgatatt gcgcggggcg gcaattgagg ccgcccggca ggcgagattc gcgcccgtgc
     5281 tggtttccgg ccagcccgtg cccgtctccg gtttcatcac ttacaccttc ggactctaac
     5341 gctagacgcc atcgctatga tcgaacaact cttcgaacag ttcttcgaac acctgcgtta
     5401 cgaacgcaac gtgagcgagc acacgctgcg caactatcgc atcgacctcg gccagttcta
     5461 cgatcatctc gcgcccgccg atgcgacgac cggcgcgcgc cgcgagttcg acctcaagca
     5521 gatcgatcac atcacgatcc gcgaatggct ctcgacgctg cacgccgcca acaaaaagaa
     5581 gacttccatc gcgcgcaaac tcgcctcctt gcgcaccttc ttccagtttc ttttgcgcga
     5641 gggcgtcgtc gagatgaacc ccgccaaact cgtctccacg ccgcgtctgg agaagaaact
     5701 tccgaaccat ctctccatcg aagaggccgt ccgcttcatc gaaacgcccg acacggaaac
     5761 cgatctcggc aagcgcgacc gcgccatcct cgaatttctc tacggcacag ggatgcgcgt
     5821 ctccgaactc gtcaagctgg atttgcgcga catcgatttc aaaaacaaga tggtgaaagt
     5881 tacgggcaag cggcgcaaac agcgcatcgt gcccttcggc gatccggcct tgcacgccct
     5941 gatgcaatac ctgaccgtgc gcggcgcgtt cctcaacaac gcgccccttg ccgaacgcga
     6001 cgagcaggta gtcttcctca attatcaggg cacgcgcatc acgacgcgct ccgtcgggcg
     6061 catggtggac aagtacatca cgatctgcgc gggcatccac aacatcagcc cgcactcgct
     6121 gcgccactcc ttcgccacgc acctcctcga cggcggcgca gacctccgcc acattcagga
     6181 gctactcggc cacgcgcgcc tctccaccac ccaaatctac acgcacgtct ccatggaaaa
     6241 attgatcgag gtctacgaca agtcgcaccc caaagcctga gagcagtgac aagtgacggg
     6301 tgacaagtga cgagaaaagg gcaagtgaaa agcagtaaat cgtgaatcgt caatcgtgaa
     6361 tagagagaag ctgtcctctt ctattcacga tttacgattc acgatttact gcttttgttt
     6421 taactcgtca cttgtcacta caaagtcgag atgtcgtcga gtccctgttg ggcttcgagt
     6481 ttggactggc aggcggcgtc gtagcgcgcg gtgggaacgg cttcgaggcg gcgttcgtca
     6541 atgggtttgc tgcaactttg gcagatgccg taggagcctt cttcgatgcg gagcaaggct
     6601 tgatcaatat cggccacggc ttgcgactcg cgttcgccga ggcggagttg gagttcctga
     6661 ttaacgtctt gcagcgacat atcgacgcta tctttcacgc cgtcgtccgc gatcatgtcg
     6721 agcgcggcct gctggttgtc tcgcacatgt tccgtgtgct ggcgtagctg cttcgtcagg
     6781 atgccgcgaa aatgttctaa acgatctgcg tccattaaag tattgccccc cgaggtagaa
     6841 ggaagttcgt tcgcttccga tgtattgtgg attcgagcag gcggattata acacagacgg
     6901 ctgcgccctc ggcgtcgctg cgcgcgcagg cgcgacggtg gcaatttttc tgtgcgcgga
     6961 agcgagaaaa tagtcggcgt cgaatctccc gccccgtcaa ttttaggttt actttatgct
     7021 ccggctacag tactttgtca ctcacctcac tacgctgcac ggctggcctt gaagaacttt
     7081 gcaagcggcg tgacccctgc tcgaagtata ctcccgaact tgaaaaagaa ctgtctcctg
     7141 ttaagcataa ccttcgcgcg gcgaattgtt tcctctcaat tctgccggaa aggagtctca
     7201 ccttggatgt gcgagtacaa aacgttgtgg ttctcatgcg acgggatttg cgcactaaac
     7261 tcacgctgca cgacatggcg cgcgccgtca atttaaccac ttttcatttc tgtcacctgt
     7321 tcaaagccga gacaggccgc agccccgcca aatatctcaa agccctgcgg ctcgaacgcg
     7381 cacgcgaatt gttggaaaca actttcctga gcgtgaaaga gatcagggcg ctcgtcggcg
     7441 tcaacgacga gagccacttc gcgcgcgatt ttcgcaacgc ttacggcctc acggcgcagc
     7501 aataccgtga gcagtttctc ggactcgacg cgcagaaaaa agatgaggcc gaacagaccc
     7561 agagcggctc ttaagatagc aattgcgaac ctgacctgca cggcttgctt gcggcgacgg
     7621 cgacgttctc aactctgctg ctaaacgcgc ggcgcacgcc aaacgtcgaa tgcgtggagc
     7681 gtgcgcgacg atctcatctg agacggaaga agggcggcga acatctcaag ttcgccgccc
     7741 ttcgtttcaa ccatccgcca ctcgctctaa aagttaaact tcaatccggc ctgcacgaga
     7801 cgtgggctgc ttggcaggat gccgcgcgag cggtcaacga tgtaggtgcg gtcgaagaga
     7861 tttttggcgg tgacgaaaaa agtcgaacgc atctgctcaa ccttgtagtt gacggctgcg
     7921 ttccagacgg cgtagccggg cacggggcca agttgcccgt cggcgcgcgg cgaggtgcgc
     7981 aaattcagat cgtcgccgaa ctgcctgccg acatagactc cttcgagcag tgcgtcgatg
     8041 ccgaccggat gcgagtagcc gatgttggcg ttcagcaaat tctccggcgc gtagggcagg
     8101 cgattgcctg tgatcgagac gcccgtgaag ccacagatgt tgctgaatcg cgtgccggtg
     8161 tacttcgcgg tggggaggaa ggtgtaggcc acgcgcaggt agaaattgtg acggttgccg
     8221 aggagcgcgc cggagtccac gcgccccgtg aattccgcgc cttgatgaag cgtcgagccg
     8281 ccgttggtca gcgcagaccc gatgccgccc gcgagagtcg ccggaacaat ctgattctcg
     8341 tagttcatgc gaaacagcgt ggcttcaaaa cggaggccgc gatgcggcgc ggcgcgcagt
     8401 ccgagttcgt agttccaact gagttcgggg tcgagttcga tgctcccgcc cgtggtgttg
     8461 ttgatgatgt cttcggtgcg cggcggcgcg aagcctcgat gcacgcccgc gaagacggtc
     8521 aaccccgcgt tcggctggaa ggagacgccg atgccgggca cgacctgcgt gagagaagtc
     8581 cggcctgaca cgccaagccc gccgttggcg agacgattcg tgcgctcgaa tccgatgtgc
     8641 tcgatgcgga ctcccggcgt gatggtgaat ttctcgaaga gcaaacggtt ttgcacaaag
     8701 gcggaatagg cttgattgcg ccgctcgtta ttttcagcga tcacgccgtc gcgcgaattc
     8761 ggcagatcgc cgttcttctg tagacgttcc tgcgtctcgt aatgcgcacg cacgccgaag
     8821 tccgtctcgc tcctgacgcc gaagagattg tgccggacgt tcaagcgcgg ctcgacgccg
     8881 aagaaatagt atttgcgcag acgcccttcg ttgccgcagg tcgtgttgag atcggcgaaa
     8941 ctgcggcaat caggatcgac gttcagacgg ttgggacgct gcgcggagtt gctcgactgt
     9001 cgccaccagt ggcggcgaaa atatgagccg taaaagttgg tcgaaagcgt cgtgttggag
     9061 ttgaagacga aggcgtgcgt ggccgacgcg ccaaagcggt cgccgaagaa gtggtcgttg
     9121 aagaacggat tgccgcgcgg gtttgcggcg tactcggcct cggtcagccc ggaatatgag
     9181 acttgcgaac gctccctgta aaagttgaac ttggtggtga gcgcctgccg cgtgccgatg
     9241 gccgtgacgg acttgaagtt gaagtcggag aggccggagc ggtgctggtc gcgtgcgccc
     9301 tcgccctgtt tgcgcgtgaa gtcgaagagc agtccggtgt tgccgtacgt gccgccgtag
     9361 ttgaggtggc cgttgaagta gtcgcggttg ccgccgatga gcgtgaggga gccggaagtt
     9421 ttttcgggag gcgtgcgcgt gatgtagttg acgacgccgc ccacggtctg cggcccgtac
     9481 acgatctgcc cggagccttt gagcacctcg acgctctcga aacgatcgat gggcgggtga
     9541 tagtaggaag cgttgtcgcc gtagggcgcg taggtgagcg ggatgccgtc ttcgaggagc
     9601 aggactttgg tggagcgcgt cgggttcaaa ccgcgcaggc cgatgttcgg gcgcaacccg
     9661 aaaccctctt cgtcgcgcac gttcacgccc gagaacttgc gcaaggcttc gctcgaagtg
     9721 aagacgcggc tgatttcgag catctcccgt tcgagcactt caaccgaacc gggaatgcgc
     9781 tcgaccactt cgggaacgcc cgcgatgcgg ctcgacgtga cgacgaccga ctcggcgagg
     9841 gaagcggcct gcaaagtgag ttcgatgttg ctctccgctc cggctttgat tcttatcacc
     9901 tggtctgcga gcgcgaagcc gcgcctcgtc gcacgcagac ggtaagagcc tgtgctcagt
     9961 ccttgaaaag tgaaagcccc tgcggcgtcc gtgttcgtcg tgctctcgtc gttcctgtgc
    10021 tcctgtctga gcgtcacgtt tgcgccttca accgccgccc cgaattgatc cagcacatgc
    10081 ccggacaacg agacgccact cgactgagcc gccgcgctcc aactacacac gcacatcacc
    10141 acgagcgcga acacaccaca cagcaatcta cgcgaggccg cactccccac acacgccgtc
    10201 cacataattt cttcccctgt caggattttg aagatgttta gaacttcggg ttgtctgaaa
    10261 ttgaaaacga ctttcaattt cgagcacggt ggaagttaaa ccgtgaagcg ttttctgtca
    10321 agcaaaacta ttgaacgagc gtttgactta agggaatggc tgcttctgta gccgtggcgg
    10381 gggcgacatg tctatagccc acgcggcgcg aggcggagca gtaagtgtct ctaatagatc
    10441 aagcccggag tggcaaaaga ttcttagact cgaatctctt tcgcgcctcc ggggttgctc
    10501 aaatttattc tcacatctct cccgccgctt aagctgtggg ctacaaatat aacgtccctg
    10561 ccgggctaag agagcggggc ggttttgtgt ctgcgcttga tgttgcccgg tggcgcgatg
    10621 tgtcgagagg cttcgtgcca gtgtgtgcgg gcgagggtta atcttggccg cagagaatct
    10681 gtgcggcgag cagggggatc atcaattcgt gatggcctgt gatggaatag ccgcgccctg
    10741 cgccgtcgct ggtggggcgg cggacgacgt ttgtgagcgg gcgataggat tggatgaagt
    10801 cgaagttggc ggtggtgaaa tcggcgagcg ggtgcttgag attgcgcacg agcgtgacgg
    10861 ccttcaaaaa tatttccggc aagacgaccg ccgagccgat gttcagatag acgccgccgc
    10921 cgtgcatgcg ccggaccagt tctgccagca ggcgaaaatc tgtgtgtgat gtttcgccga
    10981 gactcgcgcc gtgcgccgtc gggtgaaagt gcgcgatgtc tgcgccgatg gcgacgtgcg
    11041 ccgtgaaagg caggcgcgac tcgtaggctg cgcagagcag cgacagccct gcgtgcggcg
    11101 gattgagttc ggtgagggcg cgtcccaacg cttcgccgag tccgagacgg tcacgcgcgc
    11161 ccgcttgcgc cgcatcattc aaaagtctgc ccgtctcttc cgccgcgccg aacgcgcctt
    11221 ctccgagcgt ggcatcgaca tcttcggacg ttgaaccggc gagcgcgatc tccgcatcgt
    11281 ggacgagcac cgaaccgttc gcggcgatgg ccgagacgta gccgcgcttc atgagatcga
    11341 tgataacggg cgcgagtccg gtcttgatga cgtggccgcc gatgccccag atgatgggtt
    11401 tctgcaactc tcgcgcgcgg cgcacctgcg cggcgacctc tctgagagaa cgcacggcga
    11461 ggatgtttgg cagcgtgctt agaaaatcac gaagggtgga atcaactgcg ggggctttgg
    11521 caaagtccgc cgttgccact ttgctcgggc gcgaggcgag cgggtaggtg ctgaccttct
    11581 caaggtcgag cgggcgcaaa tcttcgtcgt aaattgacac ggctgcgagt cgttccttct
    11641 tcaatccccg gcttgcccgg agagcatccc ttccgcgagt tcgcacagga tgcggccaaa
    11701 caggttgtgc gtctcccgtg tgcgctacgg aaagatgcgg agatgagcgc ggcggcgcgt
    11761 tctacttcgc acgcgtgctc cgcgaagtga cgttgttcga ctttgacctt cgcggcgacc
    11821 aacaccggga tgtggttcga gcatactttg tcccgagacg agagagggca aggacggaga
    11881 cggggcggct cgttgcgcgg tcaactcgcc gccccgtttt taaaacttca cttctcgaaa
    11941 cttcacttct cgaaacttca cttgctgaaa cttcacctac cgagaatgcc cggcaggaat
    12001 tctctgatca cgttggagag cgcgccggtg acgaaggaca tgccgaagcg gcggaaggtg
    12061 gcgtcggtgc cgatgcggtc gcgtccgggc gtgctctgct cccagaggtt cgagagcgcg
    12121 ctgccggaaa gttgtccggc gaagcgcgag tagttgggct ggtgtctgcc gttgtcgcca
    12181 cgcgtgatga agacgcggct ggcggcgtgg agcgcgcgcg cggcgacgcc cctggtgcgg
    12241 tctgcacgct cgtagcgcgg gtcttgtttg aacagcgaag catagacgcc gttcgagagc
    12301 aggacggtcg tgctgcgcct gccgaacttg atggcgaagc gcgagaggcc gtcggccacg
    12361 cggtcgtccg tgtccttgtg cggcaggtct tcttcgcccc actccgtgag caccgcgctg
    12421 gcgaacggca gggcataagc ctgcggggtg agaaaggccg acctgaacgc gcgccgcatt
    12481 ttctggtttg aagtcagcgg cgtgtaggtt gcagttgccg cctggccgcc ggggttcgtc
    12541 tggttggcga atgagtctga cgacgaccga gcgtttgcgt cgaaggcgaa agaacgcgcc
    12601 gcgcccgttt taagttcgcg cggggaagag ataagacttg cgtgctgaga cgtgaaagaa
    12661 gtgcgcttgc gcgcgtcgag cagttcgacg cccggacgtt tcgccgcgtc accgttcacg
    12721 acggacgact gttcacacgc ctcgtctccg gcggtcgctt tttctccggt ggttgcgacc
    12781 gcttgtgccg ggggcggcgc agtttgagtc gcttgagaga aggcgtgtgg cgtgtttgtc
    12841 agggcgaaga ttaactgtag gaaaattacg ggcaagaatg cccggatacg aagatttgtc
    12901 accgagtttc tccttgttat cgggtgccgc tggcgtcgct tcgcaggggg agcgacaggc
    12961 tttagcacgt tgcggactgg caacatgcta cccgccggag gccatacatt tcaagcaatc
    13021 ttttttgttg cccgcgccgt cacggttttt ccgttgcgcg gcgacttctg ttcgttgttc
    13081 gcgtggccgt cgaggagggg cttgagcgcc tgcccggtgt gggatttttt gacgcgggca
    13141 acctcttcgg gcgtgccgca ggcgacgacg cgaccgccgc ccgcgccgcc ttcggggccg
    13201 aggtcgatca cgaagtcggc tgacttgatc acgtcgaggt tgtgttcgat gacgaggacg
    13261 gtgttgccga gcgagacgag gcgttggagc acgtcgagca gtttgtgtac gtctgcgaag
    13321 tggaggccgg tgg
//
LOCUS       DASWNS010000496         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_18920, whole genome shotgun sequence.
ACCESSION   DASWNS010000496 DASWNS010000000
VERSION     DASWNS010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18920"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="VGB76_17130"
     CDS             complement(<1..1794)
                     /locus_tag="VGB76_17130"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0100673.1"
                     /translation="MFPTPTYPGTLARLCLIVSLLSIFPAPALAQQHGRIKGTVRRND
                     ATAGTPLGGATVIAINQVTSNRSMTRTANDGSFSFKLRAGAYRILVDAPGAARFDKEN
                     IIVEGGRESAVEVELKALKEPQKKAEPFVDPTANERPAGYAGGGNIDSRPPTAPDRAE
                     VRDRWRINFPEYDRYGDKGARGRDIPFKRGRWYDPYNQNLLKGDFPIFGQNVFMILSG
                     VDTTTIELNRTPKPTDVSSARPGSAEFFGRPETLVANHVIQFSFEMFGGSTTFKPRNW
                     AIKISPTISLPNYVNARERGVINIDPRRGTNRTDTHVSLEEAFAEVKLEDVNANFDFV
                     SVRAGIQPFVSDFRGFIYSDNNLGARLFGGFANNQYQFNAAYFSQLEKDTNSGLNRFD
                     TRHQNVYIANLYRQDFIRKGYTAQVSFLFNDDRPSRKFDRNGFQVRPALVGDVRPHGV
                     KVGYLGFSGDGHLGGGTLGRFNLSHSYYLALGRDTHNPIAGRPVNIRSQMAALEISYD
                     RDFFRLKTSGFFAQGDKNPTDSQASGFDSIFDDPNFIGGQFSFWNRSAIRLTQTGVSL
                     VEPNSILPSLRSSKTQGQANFVNPGILIFNAG"
BASE COUNT          352 a          497 c          641 g          382 t
ORIGIN      
        1 gcccgcgttg aagatcagga tgccgggatt gacgaagttg gcctgtccct gcgtcttgct
       61 gctgcgcaaa ctcggcagga tgctgttcgg ctcgacgagc gacacgcctg tctgcgtcag
      121 gcggatggcc gaacggttcc agaaagagaa ttgaccgccg atgaaattcg gatcgtcgaa
      181 gatggaatcg aagccgctcg cttgcgagtc ggtcgggttc ttgtcgccct gcgcgaagaa
      241 gccggaagtc ttcaggcgaa agaaatcgcg gtcgtaactg atttcgagcg cggccatctg
      301 gctgcggatg ttgacggggc gacccgcaat cggattgtgc gtgtcgcgtc cgagggcgag
      361 atagtaggag tgcgagagat tgaagcgacc gagcgtgccg ccgccgagat ggccgtcgcc
      421 ggagaagcca aggtatccga ccttgacgcc gtgtggcctc acgtcgccga cgagcgcggg
      481 gcgcacctga aagccgttac ggtcgaactt gcgcgagggt cggtcgtcgt tgaagagaaa
      541 actgacctgc gccgtgtagc ctttgcgtat gaagtcctgc cggtagaggt tggcgatgta
      601 gacgttttga tggcgcgtgt cgaaacggtt gaggccgctg ttggtgtcct tttcgagttg
      661 ggaaaaatag gctgcgttga actggtattg attgttggcg aagccgccga agagacgcgc
      721 accgagattg ttgtcggaat agatgaagcc gcgaaaatcg gagacgaacg gctgaatccc
      781 cgcgcgcacg gagacgaaat cgaagttggc attcacgtct tcgagtttca cttcggcgaa
      841 ggcttcttcg agcgagacgt gcgtatcggt gcgattcgtg ccgcgacgcg gatcgatgtt
      901 gatgacgccg cgctcgcgcg cgttgacgta gttcggcagg ctgatcgtgg gcgagatttt
      961 gatcgcccag ttgcgcggct tgaaggtggt ggagccgccg aacatctcga aagagaactg
     1021 gatgacatgg ttggcgacga gcgtttccgg tcgcccgaaa aactccgcgc tgcccggtcg
     1081 cgcgctggac acgtccgtcg gcttcggcgt gcggttgagt tcgatggtcg tcgtatcgac
     1141 gcccgacagg atcatgaaga cgttctggcc gaagatgggg aagtcgccct taagcagatt
     1201 ctggttgtaa ggatcgtacc aacgcccgcg cttgaagggg atgtcgcgac cgcgtgctcc
     1261 tttgtcgccg tagcgatcat attcggggaa gttgatgcgc cagcggtcgc gcacctctgc
     1321 gcggtcgggc gcggtgggcg ggcgcgaatc aatgttgccg ccgcccgcgt agcccgccgg
     1381 acgctcgttc gccgtcgggt caacgaacgg ctccgccttc ttctgcggct ctttcaaagc
     1441 cttcaactcg acctcgaccg cactctccct gcccccttcg acgatgatgt tctctttatc
     1501 gaaacgcgcc gcgccgggcg catccacgag gatgcgatac gcgcccgcgc gaagtttgaa
     1561 agagaaactg ccgtcgttcg ccgtgcgcgt catcgagcgg ttgctcgtca cttgattgat
     1621 ggcgatgacg gtcgcgccgc cgagcggagt cccggcggtg gcgtcgttgc gacgcacggt
     1681 gcccttgatc cgtccgtgtt gttgtgcgag agcgggcgcg ggaaagatgg aaaggagcga
     1741 gactattaag cagagtcgcg cgagcgtgcc gggataagtt ggggtaggga acatgaagcc
     1801 gttataaaat gacgggtcgc tcgcaagggg cgatctcgtg cgtgtggtgc caaatgaaat
     1861 tcgtgaatta ca
//
LOCUS       DASWNS010000497         7488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_407940, whole genome shotgun sequence.
ACCESSION   DASWNS010000497 DASWNS010000000
VERSION     DASWNS010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407940"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VGB76_17135"
     CDS             complement(<1..1250)
                     /locus_tag="VGB76_17135"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0100674.1"
                     /translation="MEGIDTTRLGDIYVRREADIVKVRERVRRLAREMRFDTTTQIKI
                     TTAVSELTRNIYEYAQSGAITLAVAFRTGGSQAAGLQITARDNGPGINEADLRAIMRG
                     QYNSTSGLGVGLTGTRRLMDEFEIESAAGAGTRVTIVKWLPPAEAAGLKARIDALRAD
                     FNADDTESAVEELARQNRDFVRILAELEEKREALELLNQQLSESNTELNEANARLREI
                     AELREEFLALTTHDLRSPLTVISGVISFFTSGRLGELSPEQKNMVGMMERNTQNLIEL
                     VNDLLDAAKLESGSLQLELASTDLPALIHEICEPLEPLASEKGIALTQELPAELPPVR
                     ADRAKLRRVIVNLLSNALKFTPKGGRVAVRAEETGAGIRISVADTGVGIAAQDIPLLF
                     DKYEQARHRATRGEKGTGLGLYITR"
     gene            complement(1333..2379)
                     /locus_tag="VGB76_17140"
     CDS             complement(1333..2379)
                     /locus_tag="VGB76_17140"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0100675.1"
                     /translation="MRVIQSIEVRDEGQVGAVRRAVHAFAGGLGFSERELAEIDIVVQ
                     EVGTNAARYAKDGGQIHFTTTLGAEPGLEIFYTDQGPGIYDLERVVRDGVSTGGSLGA
                     GLGAVRRLMDEFEVYSTIQGTGRLASLARRTTHGTAILLRKWVAATRLSDKTHAATAR
                     RIGVWSRPHPEEDRNGDAYFLRRRAGQTLLAVVDGLGHGHGAHAASRVALELLAEWQG
                     ESLEDVFRNAHAALRPTRGAVMGACILDTTRGIFSYAGVGNIEVRVFNAPEPARPISN
                     NGTLGARMDKLRVWSYPWAEGSVVVLASDGVSATWDLGDYPGLLQKSPQLLAGILMRD
                     YGRDTDDATVLVAR"
     gene            complement(2554..2952)
                     /locus_tag="VGB76_17145"
     CDS             complement(2554..2952)
                     /locus_tag="VGB76_17145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEY0100676.1"
                     /translation="MESRAISLESEHDIVVARGEVRAIAVALGFRLLDQTRLATVTSE
                     LARNIVKYAGRGQVIAQPTRAPDGREGLRLIFEDSGAGIPDIPAAMRDGFSTGRGLGK
                     GLPGSQRLVDEFKIESMPGSGTRVTVVRWI"
     gene            complement(2927..3373)
                     /locus_tag="VGB76_17150"
     CDS             complement(2927..3373)
                     /locus_tag="VGB76_17150"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0100677.1"
                     /translation="MNGAQRIPILKVGRVLVVPVQVDMDDKTVIGLQERILTELERTG
                     ARGVLIDISLLEMVDSFIGRMLSDIAAMARIMDARTVVVGIQPAVAITLVELGLELRG
                     VDTVLNVDEGIKLLRDEFGADERADEDEEMDFRGELNSFGEPRHLA"
     gene            complement(3497..4450)
                     /locus_tag="VGB76_17155"
     CDS             complement(3497..4450)
                     /locus_tag="VGB76_17155"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0100678.1"
                     /translation="MPGTTKTQISGRVVELLSMRRDEILNIWIKERLESEDFRDELIS
                     KHELRQQSQQIVEMLARALQESDGADFDHPAFDELRTFLNEISRQRAVKGYTPSENAT
                     YILSLRNVVLPILAEDFEGNTVTLVREMDHFTRLLDKMGLVMVENFIRSREEIIRQQR
                     ADMLELSTPVIKVWDKILTLPIIGTLDSRRAQMMTEALLQRIVDTGSTVAILDITGVR
                     TMDTLVANHLIKTVTAARLMGARCILTGVSPAIAQTMVQLGIDLTQITTRAQMSDGIR
                     LALEMVGRKVVSAAALANYLRSANGTGSPAPKTDQTVTSDK"
     gene            complement(4741..5265)
                     /locus_tag="VGB76_17160"
     CDS             complement(4741..5265)
                     /locus_tag="VGB76_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100679.1"
                     /translation="MFQDTNLRSSLNSFRLPLLLAAFILCLAVVDAAAQTQASNANPA
                     SPAPPAAADEVPAFQEYKGVRIGMTADEARKKLGSPTDKGDAQDFYLVSPKENVQVMY
                     DASKKVSAIALIYMNAGANAPTAKAVMGTDLEAKPDGSLYKLVRYPKAGYWVSYSRTA
                     GNSPIVSVTMQKIN"
     gene            complement(5478..7193)
                     /locus_tag="VGB76_17165"
     CDS             complement(5478..7193)
                     /locus_tag="VGB76_17165"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0100680.1"
                     /translation="MSSHDQILDARMGTVKGPGERPHEFLFITADNRRARIGEFVYYL
                     ARDGANERQILGNITSRRLVRNLPDAFLSDPETPPAVISALIGLDGDGCELYEITVET
                     IGYFSRQMGDFVNPRIPPNAGDAVFLASSETLAGVLSSRRVGEHGSAHLGSLLTRNAG
                     EVPVVLSVKDVVSTHLAILASTGAGKSYTAGVLVEELMMPHNRAAVLIVDPHGEYDTL
                     RSIEGDRRFRAEADGYQPEVKLFTHDRVRVRFSSLTEADIKYLLPEGASDKMLHYLSQ
                     AYRKLTAGERGERGLWGYHDLRDAVTAEKYGEEDARGGAGAGGNVGSIDALLWRLDSR
                     FDRPGSIFSDNEHIELKELFRPGRCTVLQLSDIEQHEQQVVVATLLRRVNKARMATVR
                     GEADGSSESYLPYPVFTLLEEAHRFAPAGQTVVSTNILKQILSEGRKFGVGIGLITQR
                     PGKLDQDVLSQCMTQVIMRIVNPIDQDTVAKSVEGAGRGLLNELPALTKGQAVLSGVG
                     VNTPVMCRVRERITQHGGETFDAPEEWARWHSESERLHREESEAIVVKPTSAKKVEKI
                     RGIQI"
BASE COUNT         1376 a         2258 c         2343 g         1511 t
ORIGIN      
        1 cgcgtgatgt agaggccgag gccggtgcct ttctcgccgc gcgtggcgcg atggcgcgcc
       61 tgttcatact tgtcgaagag cagagggatg tcctgcgccg cgatgccgac gcccgtatcc
      121 gccaccgaga tgcgtatgcc cgcgcccgtc tcttcggcgc gcaccgcgac gcgcccgccc
      181 ttcggcgtga acttcaaagc gttcgagagc aggttgacga tgacgcggcg aagtttggcg
      241 cggtcggcgc gcacgggcgg caactctgcg gggagttcct gcgtgagcgc gatccctttt
      301 tcgctggcga gcggttcgag cggttcgcag atttcgtgta tgagggcggg caggtcggtc
      361 gaagcgagtt cgagttggag cgagccggat tcgagcttgg cggcgtcgag cagatcgttg
      421 acgagttcga tcaggttctg cgtgttgcgt tccatcatcc cgaccatgtt cttctgttcg
      481 ggcgagagtt cgccgagccg accggaagtg aagaagctga tgacgccgct gatgacggtc
      541 aggggcgaac gcagatcgtg cgtggtgagg gcgagaaact cttcgcgcag ttcggcgatc
      601 tcgcgcaggc gcgcgttggc ttcgttgagt tccgtgttcg actcggagag ttgttgattg
      661 aggagttcga gcgcttcgcg cttctcttcg agttcggcga ggatgcgcac gaagtctcga
      721 ttctggcgcg cgagttcttc gacggcggat tccgtgtcgt cggcgttgaa gtctgcgcgc
      781 agcgcgtcga tgcgtgcttt gagtccggcg gcctctgcgg gcggcaacca tttaactatc
      841 gtgacgcgcg tgcccgcgcc tgccgccgat tcgatctcga actcgtccat caggcgacgc
      901 gtgcccgtca gcccgacgcc gaggccggaa gtcgaattgt actggccgcg catgatggcg
      961 cgcaggtctg cttcgttgat gccggggccg ttgtcgcgcg cggtgatctg caaacccgcc
     1021 gcctgagagc cgccggtgcg aaaggcgacg gcgagcgtga tcgcgccgct ctgcgcatac
     1081 tcgtagatgt tgcgcgtgag ttcggagacg gcggtggtga tcttgatctg cgtggtcgtg
     1141 tcgaaacgca tctcgcgcgc cagacgccgc acgcgttcgc gcaccttgac gatgtcggcc
     1201 tcgcgccgca cgtagatgtc tccgagccga gtagtgtcga tgccttccat agaagtctca
     1261 agtctcaagt ccgaagtcct gagtcgcgaa gccgcgtgtg gcctcgcgac tcaagacctg
     1321 agactttgag acttatctgg cgacgagtac ggtcgcgtcg tcggtgtcgc gcccgtaatc
     1381 gcgcatcagg atgcctgcga gcagttgggg gcttttctgc aacagtccgg ggtagtcgcc
     1441 caagtcccat gtcgcgctca cgccgtcaga cgccagcacg acgaccgagc cttccgccca
     1501 agggtacgac cagacacgca acttatccat gcgtgcgccg agcgtgccgt tgttcgagat
     1561 cgggcgcgcg ggttcgggcg cattgaagac acgcacttcg atgttgccca cgcccgcgta
     1621 agaaaagatt ccgcgcgtcg tgtcgaggat gcacgcgccc atcaccgcgc cgcgcgtcgg
     1681 tctgagtgcc gcgtgcgcgt tgcggaaaac gtcttcgagc gattcgccct gccattccgc
     1741 gagcagttcg agcgcgacgc gtgaagccgc gtgcgcgccg tggccgtggc cgagtccgtc
     1801 gacgactgcc agcagcgtct gccccgcgcg acggcgtagg aaataagcgt cgccgttgcg
     1861 gtcttcttcc gggtgcgggc gcgaccagac gccgatgcgc cgcgcggtcg ccgcgtgcgt
     1921 cttatcggag aggcgcgtgg cggccaccca cttgcgcaag aggatggcgg tgccgtgcgt
     1981 cgtccggcgc gcgagcgagg cgaggcggcc tgtgccctga atcgtggaat agacttcaaa
     2041 ctcgtccatc agccgacgta ccgcgccgag tcctgcgccg agcgagccgc ccgtcgaaac
     2101 gccgtcgcgc acgacgcgtt cgagatcata aatgccggga ccctgatccg tatagaaaat
     2161 ttccagcccc ggctccgcgc cgagcgtcgt cgtgaaatga atctgcccgc cgtccttcgc
     2221 atagcgcgcg gcgttcgtcc ccacctcctg caccacgata tcgatctcgg ccaactcgcg
     2281 ttcgctgaaa ccgaggccgc ccgcaaacgc gtgcacggcg cgccgcaccg cccccacctg
     2341 cccctcgtcc ctcacctcta ttgattgaat aactctcata aataaagtga tgagtaatga
     2401 gtgatgagtg atgagtgata aatgaaaaag ccttgaatct tgaagcggca atcatgaata
     2461 gacgaagaac tgatctcctc tattcacagt tgccgattca agattcactt gcttgtaact
     2521 catcactcat tactcatcac tcatcacttc ttttcaaatc caacgcacga ccgtcacgcg
     2581 cgtgccgctt ccgggcatgg actcgatctt gaactcgtct acgagacgct gcgagccggg
     2641 gagtcctttg ccgaggccgc gacccgtcga gaagccatcg cgcatggcgg cggggatgtc
     2701 tgggatgcct gcgcccgaat cttcgaagat gaggcgtaag ccttcgcgcc cgtccggcgc
     2761 gcgcgtcggc tgcgcgatca cctgtccgcg ccccgcgtat ttgacgatgt tgcgtgcgag
     2821 ttcggaggtg acggtggcaa gacgcgtctg gtcgaggagg cgaaagccga gtgcgacggc
     2881 aatcgcgcgc acctcgccgc gcgcgacgac gatgtcgtgc tccgactcaa gcgagatggc
     2941 gcggctctcc aaacgaattt agctccccac ggaaatccat ctcctcatct tcgtccgcgc
     3001 gttcgtccgc gccgaattcg tcgcgcagca gcttgatgcc ttcgtccacg ttcaggacgg
     3061 tgtccacgcc gcgcagttcc aagccgagtt cgacgagcgt gatggcgacc gccggttgga
     3121 tgccgacgac gacggtgcgc gcgtccatga tgcgcgccat cgccgcgatg tccgagagca
     3181 tgcgcccgat gaaggaatca accatctcca gcagcgagat gtctatgagc acgccgcgcg
     3241 cgcccgtgcg ttcgagttcc gtcagtattc gttcctgcaa tccgatgacg gtcttgtcgt
     3301 ccatgtccac ctgcaccggc acgacgagga cgcggccaac tttcagaatc ggaatacgct
     3361 gcgctccatt cataagcatt actccgtaat gcagtgacag gtgacgggtg acaggtgaca
     3421 ggctaagaaa acagggacgc gggacaagtg gcaagttaaa gccgcaatct tgtctttcat
     3481 ctcgtctttc atcccgtcac ttgtcacttg tcactgtttg atcggtcttt ggcgcgggcg
     3541 agccggtgcc gtttgcgctg cgcaggtaat tggcgagcgc ggcggcggag acgaccttgc
     3601 gcccgaccat ttcgagcgcg agacggatgc catcggacat ctgcgcgcgg gtggtgatct
     3661 gtgtcaggtc gatgccgagt tgcaccatcg tctgcgcgat ggccggggag acgcccgtga
     3721 ggatgcagcg cgcgcccatc agacgcgccg ccgtcacggt cttgatcaaa tgattggcga
     3781 cgagcgtgtc catcgtgcgt acgcccgtga tgtcgaggat ggcgacggtt gagccggtat
     3841 cgacgatgcg ttgcaggagc gcttcggtca tcatctgcgc gcggcgcgaa tcgagcgtgc
     3901 cgatgatggg gagggtcaaa atcttgtccc acactttgat gacgggcgtg gagagttcca
     3961 acatgtcggc gcgttgctgg cggatgatct cttcgcgcga gcggatgaag ttttcaacca
     4021 tcaccagccc catcttatcg agcaggcgcg tgaagtgatc catctcgcgc acgagcgtca
     4081 cggtgttgcc ctcgaagtcc tcggcgagga tggggagcac gacgttgcgg agcgaaagga
     4141 tgtaggtcgc gttctccgaa ggcgtgtagc ctttgacggc gcgctggcgc gaaatttcgt
     4201 tgagaaaggt gcgcagttcg tcgaacgcgg gatggtcgaa gtccgcgccg tcagattcct
     4261 gtagggcgcg cgcgagcatc tcgacgatct gttgcgactg ctggcgcagt tcgtgtttcg
     4321 agatcaactc gtcgcgaaag tcttccgatt cgagacgctc tttgatccag atattgagaa
     4381 tttcatcgcg gcgcatggag agcagttcca cgacgcggcc tgagatttga gtcttcgttg
     4441 taccgggcat ccgtccacct ctgatgtgtc gcgctcgcca gaagatagcg cgcagtcgtt
     4501 acaaaaactt cgcgtgagaa tgaaaaatta ccgccgtgcg tagggagaga acggcatcgg
     4561 gcgcaacatt gttggggtca agcaggatta tcttagcatc gccggggcgg gtaaaagcaa
     4621 atgcggggac gaattgaggg atgagggatg agggatgagg gatgaatcag aaaaagcatt
     4681 caacctcttg agtgcttttt ctgattcatc cctcatcctt catccctcat ccctttcttc
     4741 ttagttgatc ttctgcatcg tgacggagac gatgggcgaa ttgcctgcgg tgcggctgta
     4801 ggaaacccag taaccggcct tcgggtagcg caccaatttg tagagcgagc cgtcgggttt
     4861 cgcttcgagg tcggttccca tgacggcttt ggcggtcggc gcgttcgcgc ccgcattcat
     4921 gtagatgagc gcaatggcgg agactttctt cgaggcgtca tacatcacct gcacgttctc
     4981 cttcggagag acgaggtaaa aatcctgcgc gtcgcctttg tcggtcggcg agccgagttt
     5041 cttgcgtgct tcatccgccg tcatgccgat gcgcacacct ttgtactcct gaaaggcagg
     5101 gacttcgtct gcggcggcgg gtggggcggg ggacgcaggg ttcgcgttgc ttgcctgcgt
     5161 ctgcgccgca gcgtctacga cggcgaggca gaggataaac gcggcgagca ggagcgggag
     5221 gcgaaacgaa ttgagagaag aacgtaaatt cgtgtcttgg aacatggctg gagactcctt
     5281 ttaggtggca acaaacttta gcggtcaggg caaccgcgct gtgaaatctt gtagcgaccg
     5341 tgcagtagaa atgcggggca ggtgttagac cagagtgcaa cttccttgta cggccacttc
     5401 cgtgtcaaac cttcggtggc gagaagatat tactgccgga gggcgcaagt caatagtgaa
     5461 gttaagacgg gcggcggcta aatctgaatc ccgcgaatct tctccacctt cttcgccgag
     5521 gtcggcttga cgacgatggc ctcgctctct tcgcggtgga ggcgttcgct ctcggagtgc
     5581 cagcgcgccc actcttccgg agcgtcgaag gtttcgccgc cgtgctgcgt gatgcgctcg
     5641 cgcacgcggc acatgaccgg cgtgttgacg ccgacgccgc tcaagaccgc ctgccctttc
     5701 gtcaacgccg gaagctcgtt caacaacccg cgccccgcgc cttccacgga cttcgccacc
     5761 gtgtcctgat caatcgggtt gacgatgcgc atgatcacct gcgtcatgca ctgcgagagc
     5821 acgtcttgat cgagtttgcc cggacgctgc gtgatgagtc cgatgccgac gccgaacttg
     5881 cgcccctccg aaagtatctg cttgaggatg ttcgtcgaaa caacggtctg cccggcgggc
     5941 gcgaagcgat gcgcttcttc gagcagcgtg aagaccggat agggcaggta gctctcgctc
     6001 gacccgtcgg cctccccgcg cacggtcgcc atgcgcgcct tgttgacgcg gcgcagcagg
     6061 gttgcgacga ccacctgctg ttcgtgctgt tcgatgtcag agagttgcag cacggtgcaa
     6121 cgaccggggc ggaagagttc tttgagttcg atatgctcgt tgtcagagaa gatcgagccg
     6181 ggacgatcaa agcgcgaatc gagccgccag agcagcgcgt caatcgagcc gacgttgccg
     6241 cccgcgcccg cgccgccgcg cgcgtcctct tcgccgtatt tctccgccgt caccgcatcg
     6301 cgcagatcgt gatagcccca caatccgcgc tcgccgcgtt cgcccgccgt cagcttgcgg
     6361 taagcctgcg agagatagtg caacatcttg tcgctcgcgc cttcgggcag aaggtacttg
     6421 atgtcggcct cggtgaggga agagaaacgc acgcgcacgc ggtcgtgcgt gaagagctta
     6481 acttcgggtt gatagccgtc cgcctctgcg cgaaaacgcc ggtcgccttc aatcgaacgc
     6541 agcgtgtcgt attcgccgtg cgggtctacg atgagcaccg ccgcgcggtt gtgcggcatc
     6601 atcaactctt cgacgagcac gcccgccgta taactttttc ccgcgcccgt cgaagcgagt
     6661 atggcgagat gcgtagagac gacatctttg accgagagca cgacgggcac ctcgcccgcg
     6721 ttgcgcgtca acaggctgcc gagatgcgcc gaaccgtgct cgcctacgcg ccgcgaactg
     6781 agcacgcccg caagcgtctc ggaagaagcg aggaagaccg catcgccagc gttcggcggg
     6841 atacgcgggt tcacgaaatc gcccatctga cggctgaaat aaccgatagt ctccaccgtg
     6901 atctcataaa gctcgcaacc atcgccgtcg agtccgatga gcgcggagat gacggcgggc
     6961 ggcgtctccg ggtcggacaa aaacgcatcg ggcaggttgc gcacgagtcg tctcgaagtg
     7021 atgttcccca gaatctgccg ctcgttcgcg ccgtcgcgcg cgagataata gacaaactcg
     7081 ccgatgcgcg cgcgccggtt gtccgccgtg atgaaaagaa actcgtgcgg tcgctcgccc
     7141 ggccccttca cagtgcccat gcgcgcgtcc aatatttgat cgtgagaact catctgtgac
     7201 ttcgattatt ataacttgtg atgctgcggc gacattcggt agcgagcgtt tacagggaaa
     7261 ctaacgcggg caattatatc acgccagaca aaagccataa tgattcgtag ttctgaaata
     7321 aactccagac ggcatcaaac gccgcgcgcg gagacactct ggccgcactc cttctgcgcc
     7381 tccttcatgt ccgcctccac catctccttg atcatctccc caaacgtata ctccggctcc
     7441 caccctagct cccgcttcgc cttccccgca tccccgatca gcaaatct
//
LOCUS       DASWNS010000498         7637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_252519, whole genome shotgun sequence.
ACCESSION   DASWNS010000498 DASWNS010000000
VERSION     DASWNS010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252519"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..370)
                     /locus_tag="VGB76_17170"
     tRNA            complement(289..370)
                     /locus_tag="VGB76_17170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Leu,seq:gag)
     gene            complement(444..881)
                     /gene="secG"
                     /locus_tag="VGB76_17175"
     CDS             complement(444..881)
                     /gene="secG"
                     /locus_tag="VGB76_17175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY0100681.1"
                     /translation="MTYFLYGLFILSCIVLVVAVLLQPGKADAGALFTSSVSSTAFGP
                     RGTQTILAKITIVAAALFMLVALTLSLPLVTGSRSVVSEEAETTPAQPAASPAASPAQ
                     ENAAPVVTTETPGGSTPAANTNAANSNTGGSGATPQASPQQNK"
     gene            complement(910..1668)
                     /gene="tpiA"
                     /locus_tag="VGB76_17180"
     CDS             complement(910..1668)
                     /gene="tpiA"
                     /locus_tag="VGB76_17180"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY0100682.1"
                     /translation="MRKPVIAGNWKMYKLVAESVATALSLRPLVANVNHCEVIIAPPF
                     TSLKTVADRLEGSNIRIAGQDCSTEIKHSAHTGEIAADMLKDVGATHVIVGHSERRQH
                     YFETDTFVNLKTHAALSSDLTAIVCVGETLAQRESGNAESVVRGQVEGGLRGLTSSDL
                     ARIIIAYEPVWAIGTGRTATPEQAQEMHAFIRRVFAERHSQELADALRILYGGSVKPD
                     NIAGLMKQPDVDGALVGGASLEAEAFAQIVHYNA"
     gene            complement(1747..2961)
                     /locus_tag="VGB76_17185"
     CDS             complement(1747..2961)
                     /locus_tag="VGB76_17185"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY0100683.1"
                     /translation="MKLSIKDLDLKGQRVFVRVDFNVPLKEGVVEDDTRVRGALPTIQ
                     YATEQGARVILASHLGRPKGERVEKYSLRPVAAHLSKLLGSEVAFAEDCTGEAAQAKV
                     DALKDGDVLLLENLRFHAEEEKNDDAFAKELASLCDGLYVNDAFGAAHRAHASTAGIT
                     KHVQQAAAGLLMQKELDYLGRVIDAPEHPFVAILGGAKVSDKIAVIEALIERRVDKLL
                     IGGAMAYTFFKAEGFTVGKSLVEDDKLETAREIRERARAAGVELLLPTDHQVVDSYEP
                     LKSKKTIPIEFTNAGLVGLDIGVETVAHFTQALADAKTIIWNGPMGVFEEKELGFDQG
                     TIGIAHAVADAADRGATVIVGGGDSVAAITQVGVADRITHISTGGGATLEFLAGDELP
                     GVAALNNRKDEG"
     gene            complement(3061..4068)
                     /gene="gap"
                     /locus_tag="VGB76_17190"
     CDS             complement(3061..4068)
                     /gene="gap"
                     /locus_tag="VGB76_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY0100684.1"
                     /translation="MAIKVGINGFGRIGRNILRTALGDKEIDFVAVNDITDTKTLAHL
                     LKYDSVLGNLEQEISSTDNSITVDGDEVRVFSERDPALIDWESVGAEIVIESTGRFTN
                     AEDARKHLRGTVKKVIISAPAKNEDITIVLGVNGDKYDAAAHHIISNASCTTNCLAPV
                     AKVIHENFRIKTAQMTTIHSYTNDQQLLDLPHKDMRRARAAALSMIPTSTGAAKAVAL
                     VLPELKGKFDGISVRVPTPNVSLVDVVMEVETETTTEAVNTALKNAAGEELQGILAFS
                     EEPLVSIDFRGNPYSSIVDAEYTKVIGGKLVKVLSWYDNEWGYSCRVRDLVKFIAQKG
                     L"
     gene            4578..6566
                     /locus_tag="VGB76_17195"
     CDS             4578..6566
                     /locus_tag="VGB76_17195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0100685.1"
                     /translation="MFSYRMRSTLQTVMLWLALCAVVSAQTPVPTPQTPQSPAQRDAM
                     RPPGSEQRQTLPDQTRPTDPITPPGTQRISPQVPPGTNSPVQSPVTPSQQQPGVLAPG
                     TTPTPAQPSDAVQTPGASVPDPLFPQAQRRPVPPLPSLVRLGVESSNTLALTLDEAIR
                     RALANNNDIEVARNDVRFAETTLLQFQGIYDPVLTFTPELNNSVRPVPNIFGGAGQSG
                     TVSVTDIINNVSLTKQFAKGGGNYTYFFNNTREKTSATNSTLTPFYSSTQGVTFTQPL
                     LRNRSIDINRRQIRIQRKRLEQSDADFRRRTIEVISQVQSAYWDLVFALRDEQNQIAN
                     VNLARENFRRTEASVAAGASAPLERAEVQTELSNRESSLLLASQQVSFAENVLKQLII
                     KDPLAAEWSAAITPTDQPNFDETPVNLNTALTEARANRQELRRLKLDDEISQIDIQYF
                     KNQTKPRIDLTGTLSTNGLAGTPGLVRDPSTGLTGQIPACTGNEQTGQACLPPSFIGG
                     YGQNLKNVFSLNTRSIVVGATLQIPFGNRAAKAALTGAQIQKERLVALTRAQEQSIEV
                     DVRNAAQAVETARRRVLSARQARENAELQLSGEQRLYQVGRSTTFILFQRENQLVNAR
                     NQELRAETDYNKALADLQRATSTTLRANNVIVETPTLP"
     gene            6754..7533
                     /locus_tag="VGB76_17200"
     CDS             6754..7533
                     /locus_tag="VGB76_17200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0100686.1"
                     /translation="MKTTATIITFNEADYIRAACESVNWADEILVVDSESTDATREIA
                     EACGARVICRSWPGFAAQKQFAAEAAQHEWIFSLDADERVSSELRASIEDLLYTTDEQ
                     LADGYRVARRSFYMGRWIRGGGWYPDHQLRFYRRSHGAWRGPHVHESVQMQAGARVET
                     LPGDLLHYTGRDAAYHHRMIGERYAPLAARQMFEAGRRTSAFDVALASPAAFARSYLL
                     KGGFRDGFAGFCIARFAAHHAFLKKLLLWEMQKKQKPEGRD"
BASE COUNT         1516 a         2392 c         2196 g         1533 t
ORIGIN      
        1 ccgagcggcc tgaacgcaca ctaccaaccc aaagatttag cacgcctgtt tcgtgaacta
       61 cgcctcgcag tggagacagc ggcaggctaa caacctctcg cttgaatagg tcgaaataag
      121 gggagtcagg ataatttatc ctaactcccc cttcttatct cacacatatt catgttgagg
      181 atgaatacgg ccaccgtgct ctctcccgca tagcacggtc ttctcaagga gaaaataaaa
      241 acacccggct ctagatgttg tagcaaaaca tctagagccg ggtgtcgatg ccgaaggggg
      301 gactcgaacc cccacgagtt acctcacacg cccctcaaac gtgcgcgtct accaattccg
      361 ccacttcggc tgattaaatt gtcaaagtcg gacgctgcgg cgagtcgaag cctgtcgccc
      421 gcgcgacaga ggaatcgcgc ctcttacttg ttctgttgcg gcgatgcctg tggcgtcgcg
      481 ccgctgccgc cagtgttgct gttagcggcg ttagtattcg cggctggcgt gctgccaccc
      541 ggcgtctcag tcgtcacgac gggcgcggcg ttttcctgcg cgggcgacgc ggcgggcgaa
      601 gctgccggtt gcgccggagt cgtttcggct tcttcggaga caaccgaacg cgaacccgtg
      661 acgaggggca acgagagcgt taaagcgacg agcatgaaga gcgcggcggc gacaatcgta
      721 atcttcgcca gaatggtctg cgtgccgcgc ggcccgaagg ccgtgctcga caccgaactc
      781 gtgaagagcg cgcccgcgtc ggccttaccc ggctgcaaga gcactgccac gaccagcacg
      841 atgcaggaca gtataaaaag cccgtataag aaataggtca acaaatctcg atcctcgatt
      901 cacattaact tatgcgttgt aatgtacgat ctgcgcaaag gcttcggcct ccaaactcgc
      961 accgccgacg agcgcgccgt caacgtccgg ctgcttcatg agtccggcga tgttgtcggg
     1021 cttgaccgaa ccgccataga ggatgcggag cgcatcggcc aactcctgcg aatgtctctc
     1081 cgcgaaaacg cgccggataa acgcatgcat ttcctgcgct tgttccggcg tcgcggtgcg
     1141 tccggtgccg attgcccaga ctggttcgta cgcgatgatg atacgggcga ggtcggagga
     1201 tgtcaaaccg cgcaagccgc cctctacctg cccgcgcacg accgattccg cgttcccgga
     1261 ttcgcgttgc gccagcgtct cgcccacgca aactatcgcc gttaaatcgg aactgagcgc
     1321 ggcgtgcgtc ttgagattga cgaacgtgtc agtctcgaaa taatgctggc gacgctccga
     1381 gtgtccgacg atcacgtgag ttgcgcccac gtctttcaac atgtctgcgg cgatctcgcc
     1441 ggtatgcgcg ctgtgcttga tctcggtcga gcaatcctgt ccggcgatgc gtatgtttga
     1501 gccttccagc cgatccgcga cggtcttgag agaggtgaac ggcggcgcga tgatcacctc
     1561 gcaatggttc acgtttgcca cgagcgggcg caaactgagc gcggtcgcca ccgattcggc
     1621 gaccaattta tacatcttcc agtttccggc gatgacaggt ttacgcaata taaaattagg
     1681 gataagggac gagggataag gtatgaaaca aagacggctc tgcttctcct attcatccct
     1741 catctctcat ccctcatcct ttctattgtt gagtgcggcg acgccgggca gttcgtctcc
     1801 cgcgagaaat tcgagggtcg ctccgccgcc cgtggagatg tgcgtgatgc ggtcggcgac
     1861 gccgacctgt gtgatggcgg ccaccgaatc gccgccgccg acaatgactg tcgcgccgcg
     1921 atccgccgcg tcggctaccg cgtgcgcgat tccgatggtg ccttgatcga agccgagttc
     1981 tttctcttcg aagacgccca tcgggccgtt ccagatgatc gtcttcgcat ccgccagagc
     2041 ctgcgtgaaa tgtgcgaccg tctcgacgcc gatgtcgagg ccgacgagtc cggcgttggt
     2101 gaattcaatc gggatcgtct tcttactttt caaaggctcg tagctgtcaa cgacctgatg
     2161 atccgtcggc agcaacagtt cgactccggc tgcccgcgca cgttccctga tctcgcgcgc
     2221 cgtctccaac ttgtcgtctt cgacgagcga tttgcctacc gtgaagcctt cggccttgaa
     2281 gaacgtgtag gccatcgcgc cgccgatgag cagtttatcc acgcgccgtt cgatgagcgc
     2341 ctcgatgacc gcgatcttgt cggacacttt cgcgccgccg agaatggcga cgaaaggatg
     2401 ttcgggagcg tcgatcacgc gaccgagata atcgagttcc ttctgcatca acagacctgc
     2461 ggcggcttgc tggacgtgct tcgtgatccc tgcggtcgaa gcgtgcgcgc ggtgcgccgc
     2521 gccgaacgca tcattgacat aaaggccgtc gcacaaactc gccaactctt tcgcaaacgc
     2581 gtcgtcgttc ttctcttcct ccgcatgaaa gcgcagattt tcgagcagca acacgtcgcc
     2641 gtctttgagc gcgtcaactt tcgcctgcgc cgcctcgccc gtgcaatctt cggcaaaggc
     2701 cacttcactg cccagcaact ttgagagatg cgcggcaacg gggcgcaggc tgtacttctc
     2761 cacgcgctcg cccttcggcc tgcccagatg cgaagcgaga atcacacgcg cgccctgctc
     2821 cgtcgcatac tgaatcgtcg gcagcgcgcc gcgcacgcgc gtgtcgtctt ccaccacgcc
     2881 ctctttcagc ggcacgttga aatcgacgcg aacgaaaacg cgctggccct tcaagtcgag
     2941 gtctttgatt gataacttca taaaaacagt gacaagtgac gagtgacaaa tgacgagttc
     3001 aaagacaagt ttgaagacag gaatgctgct ttaacttgtc acctgtcact cgtcactgct
     3061 ctacagtccc ttttgggcga tgaacttgac gaggtcgcgg acgcggcagg agtagcccca
     3121 ttcgttgtcg taccacgaga ggactttgac gagtttgccg ccgatgactt tggtgtattc
     3181 ggcgtcaaca atggacgaat acgggttgcc gcgaaaatct atcgagacga gcggttcttc
     3241 cgagaaggcg aggatgcctt gcagttcttc gcccgccgcg ttcttgagcg cggtgttgac
     3301 cgcttcggtc gtcgtctccg tctccacttc catcaccacg tcaacgagcg acacgttcgg
     3361 cgtcggcacg cgcacgctga tgccgtcgaa tttgcctttg agttcaggca ggacgagcgc
     3421 gacggctttg gccgcgccgg tcgaagttgg gatcatggag agcgcggcgg cgcgtgcgcg
     3481 gcgcatgtct ttgtggggca agtcgagcaa ctgctggtcg ttcgtgtagg agtggatggt
     3541 cgtcatctgc gcggtcttga tgcgaaaatt ttcgtgtatg actttggcga cgggtgcgag
     3601 gcaattggtc gtgcaggaag cgttcgagat gatgtggtgc gccgccgcgt catacttgtc
     3661 gccgttgacg cctaagacga tggtgatgtc ctcgttcttg gcgggcgcgg agatgatcac
     3721 tttcttgacc gtgccgcgca ggtgtttgcg cgcatcttcg gcgttggtga agcgacccgt
     3781 ggactcgatc acgatctcgg ctccgaccga ctcccaatca atcaacgccg gatcgcgttc
     3841 ggagaagacg cgcacttcgt cgccgtccac tgtgatgctg ttgtcggtgg aagagatctc
     3901 ctgttcgaga ttgccgagga cggaatcgta tttcaagagg tgtgcgagcg tcttggtgtc
     3961 tgtgatgtcg ttgacggcga cgaagtcaat ctctttatct ccgagcgcgg tgcgcaggat
     4021 attgcgcccg atgcgaccga aaccgttgat cccaactttt attgccattg ttaatccagc
     4081 ctcctcacca acgttatgat ttaagagcga cgcgcgtgat gcggtcgaaa tccggcggcg
     4141 catcggcaca cggcgcgccg cgcccggacg ggatgcttgt gctcttaaag gcaaaaacta
     4201 tgttgcaagt taagatttgc aagataatgc gcgcgtgcgg cgactgtcaa ggaaggccgc
     4261 tgcacgccgg agcacgcgca ttgatacaat ttgtgccgga ataagatcaa tagccatttc
     4321 gaaattggga tgctccattc acgttttcgc gaatcgcgcc cggcggcacg ccggagaaag
     4381 ccgcgcgctg ccccttaaaa gctctcttcc agcgtggcct ctaacttgct ctttagcaat
     4441 atgtattttg aaactccgcg ctgatacgtg tcgtagtagt caactgaccg gctacgctct
     4501 gatttgaagg gcttgcagaa gttatcaacg gcgtggtgag tctttacctt acgtttccct
     4561 gaggagtttt cggtttgatg ttcagctatc gtatgcgttc aaccttgcag accgtcatgt
     4621 tgtggctggc gctctgcgcc gtcgtctcgg cacaaacccc tgtgcccact ccgcagaccc
     4681 cgcagtcgcc cgcgcagcgt gatgccatgc gcccgcccgg ctcggagcag cggcagacgc
     4741 tgcccgacca gacgcgcccc acagacccga tcaccccgcc gggcacgcag cgtatctcgc
     4801 cgcaagtccc gccgggcacg aatagccctg tgcagtctcc ggttacgcca tcgcagcagc
     4861 aaccgggcgt gctcgcaccc ggcacgacgc cgactcccgc gcagccttcc gacgccgtgc
     4921 aaactcccgg tgcgtctgtg cccgatcctt tgttcccgca ggcgcagcga cgccccgttc
     4981 cgccgctgcc gtcgctcgtg cgcctcggcg tggagagcag caacacgctc gcgctcacgc
     5041 tcgacgaagc gattcgccgc gcgctcgcca acaataacga catcgaagtg gcgcgcaacg
     5101 atgtgcgctt cgccgagacg accttgctcc agtttcaggg catctacgat ccggtgctca
     5161 cttttacgcc cgagttgaat aactccgtgc gtcccgtccc gaacatcttc ggcggcgcgg
     5221 ggcagtccgg caccgtctcc gtcacggaca tcatcaacaa cgtctcgctc accaaacaat
     5281 tcgccaaagg cggcggcaac tatacctatt tctttaacaa cacgcgcgag aagacttccg
     5341 ccaccaactc gacgctgacg cccttctact cttcgacgca gggcgtcacc ttcacgcagc
     5401 cgcttctgcg aaaccgctcg attgacatca accgtcgcca gattcgcatc cagcgcaagc
     5461 ggctcgaaca atcggacgcg gattttcgtc gccgcaccat cgaagtgatc tcgcaggtgc
     5521 agagcgccta ctgggatttg gtctttgccc tccgcgacga gcagaaccag atcgccaacg
     5581 tgaatctggc gcgcgaaaac ttccggcgca cggaggcgag cgtggccgcc ggtgcgtctg
     5641 cgccactcga acgcgccgaa gtgcagacgg aactctcaaa ccgcgagtcg tctctcctgc
     5701 tcgcctcgca acaagtctcc ttcgccgaaa atgtgctcaa gcagttgatc atcaaagacc
     5761 cgctcgcggc tgaatggtct gcggccatca cgccgaccga tcaaccgaac ttcgacgaga
     5821 cgcccgttaa tctcaacacc gcgctcacgg aggcgcgcgc gaaccggcag gaactgcgtc
     5881 gcctgaaact cgacgacgag atcagccaga tagacattca gtacttcaag aaccagacga
     5941 aaccgcgcat tgatctcacc ggcacactct cgacgaacgg actcgcgggc acccccggtt
     6001 tagtcaggga cccctcgacc ggactgacgg gtcaaatccc ggcatgtacg ggcaacgagc
     6061 agacgggaca ggcgtgtcta ccgccgagtt tcatcggcgg ttacggtcag aacttgaaga
     6121 acgtctttag tctcaatacg cgcagcatcg tcgtcggcgc gacgctccag attcccttcg
     6181 gcaaccgcgc cgccaaagcc gcgctcacgg gcgcgcagat tcagaaggaa cgcctcgtcg
     6241 ccctgacgcg tgcgcaggaa cagagcatcg aagttgatgt gcgcaacgcg gcgcaggccg
     6301 tcgagacggc gcgacggcgc gttctctccg cgcgtcaggc gcgtgagaac gccgaacttc
     6361 aattgagcgg cgagcaacgc ctctatcagg tgggacgttc caccaccttt atcctgttcc
     6421 aacgcgagaa ccaactcgtc aacgcgcgca atcaggaatt acgcgccgag acggactaca
     6481 acaaagctct cgccgatctc cagcgcgcga cttcgaccac gcttcgcgcc aataacgtga
     6541 tcgtcgaaac gccgacgctg ccataagccg gaacgcatcc ggcacgaccg actgccgacg
     6601 gcaatggccg gacgacgctt gaacagcaat gctctcaagc gtcgtccggc cattaacttt
     6661 ccggacgatt gacgcctccc cgccccgctt cgtatcctcc ctacgcgcca gaaaaacccg
     6721 aatcgctaca aacccgcgca gacgttcatc aacttgaaga ctaccgccac gatcatcacc
     6781 ttcaacgaag cggactacat tcgcgcggcc tgcgagtccg tcaattgggc ggatgagatt
     6841 ctggtcgtcg attcggagtc tacggacgcg acgcgcgaga tcgccgaagc gtgcggagcg
     6901 cgcgtcatct gtcgctcgtg gccgggcttt gccgcgcaga aacagtttgc cgccgaagcc
     6961 gcacaacacg aatggatttt cagtctggac gcagacgagc gcgtctcgtc cgagttgcgc
     7021 gcgtctatcg aagacctcct ctacacgacg gacgagcaac tcgcggacgg ctaccgcgtc
     7081 gcacgccgct ccttctacat gggacgctgg attcgcggcg gcggctggta tcccgatcac
     7141 cagttgcgct tctatcgccg ctcgcacgga gcgtggcggg ggccgcatgt ccacgagtcg
     7201 gttcagatgc aggccggggc gcgcgtggag acgctcccgg gagatttgct gcactacaca
     7261 gggcgcgacg cggcctatca ccatcgcatg atcggcgaac gctacgcccc gctcgccgcc
     7321 cgccagatgt ttgaagcggg caggcgcact tccgccttcg acgttgcgct cgcctcgccc
     7381 gccgccttcg cgcgtagtta cctgctcaaa gggggcttcc gcgacggctt cgcgggcttc
     7441 tgcatcgcac gcttcgccgc gcaccacgcc ttcctcaaga aattattgct atgggaaatg
     7501 cagaaaaagc agaagccgga gggtagagat taaaggtcgg aaggtgaagg tcagcggttg
     7561 acttaggagt tcaggcggag ttcaggcgga gttcaggcag cgcggctcgc cgttttggtc
     7621 gaagtggttg aagttcg
//
LOCUS       DASWNS010000499         5240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_96581, whole genome shotgun sequence.
ACCESSION   DASWNS010000499 DASWNS010000000
VERSION     DASWNS010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96581"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VGB76_17205"
     CDS             <1..814
                     /locus_tag="VGB76_17205"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0100687.1"
                     /translation="LGAIAALFMFSWTALFVTLALWWISGSLGIGMGYHRLLTHRGFK
                     TPKWVEYVLTTCAVLTLEGGPIQWIGTHRIHHAFTEKPGDPHSPREGTWWAHMGWILT
                     GTAQQHDEVTLRRYAPDLMKDPFHVWMSKWFFVPVVALGVLLLVFGGWSVFFWGMFFR
                     TTFGLHATWLVNSATHLWGTKRYKTEDDSTNNWIIALLTFGEGWHNNHHAHPRAARHG
                     YHWYEIDMNWYGIRALQMLGLAHDIKLLPREVKAQSDEDNDAGSPDALSQAA"
     gene            1003..1929
                     /locus_tag="VGB76_17210"
     CDS             1003..1929
                     /locus_tag="VGB76_17210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0100688.1"
                     /translation="MRFSGRRRKSIAFFITLGSCLVALAVALNVGWILLNWREVALLV
                     LGIIFFSVIIAGLVLNTTFLVREIRRNEQHDAFINAVTHELKTPITSIRLYLETLQTR
                     DVDEQKRREFYAVMLADSDRLLHTVEQVLRAGQSGGDTARRKRASEPVIDLAAIVRDS
                     LELARTRYPQLAGGAFSYAEKFRNGERALVRGDAEELRAVVSNLLDNAVKYSPDEVRV
                     SVQVMAIDARRLAVRVRDQGIGIPQAQLKRIFRRFYRVPGRLMARFKGTGLGLFIVQS
                     LVKKHGGRVYAESEGVGRGSTFTILLPRAERG"
     gene            1926..2753
                     /locus_tag="VGB76_17215"
     CDS             1926..2753
                     /locus_tag="VGB76_17215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0100689.1"
                     /translation="MKANVLIVEDERHLADGLRFNLEAEGYAVRTIADGETALALLTN
                     PAEARFDVVVLDVMLPGMNGFEVAAELRRANQFVPVLMLTARGRPEDVLRGFEAGADD
                     YLPKPFELSILLARLRGLLRRRAWLHQDQEQTAAAATPDNHALTNGATKTAVDETTKE
                     AAQRAAEESFEFDGKTIDFDALELRVRERTVRLTLMEADLLRHLIRHAGRAVSRKSML
                     EEVWNLRDDTDTRAIDNFIVRLRKYIEEEPAKPRHLLTVRGVGYRFIPNPKAATSDK"
     gene            complement(2842..3174)
                     /locus_tag="VGB76_17220"
     CDS             complement(2842..3174)
                     /locus_tag="VGB76_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEY0100690.1"
                     /translation="MQALGMVECMGLVAMIEAADAMVKAANVELVGYEKIDAGLVTAI
                     VRGEVGAVRAAVDAGAAAASRVGKVVATHVIPRPHAEVDDGLPVLHTGETTRKKISGS
                     VRAENSDK"
     gene            complement(3220..4587)
                     /locus_tag="VGB76_17225"
     CDS             complement(3220..4587)
                     /locus_tag="VGB76_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEY0100691.1"
                     /translation="MEYSQAEILQTVRMTEMEHLDVRTVTLGLSLRDCAGDSIERTAE
                     RVRAKIERTAERLVSTVDEISDELGISVANKRISITPASHIAGGARTVADCLTLARAI
                     DAAATHVGVDFLGGYSALVQKGWTDVEDIFLDSIPEALATTKRLCSSVNVATTRAGIN
                     MDAVARVGHLIKEAAELTRDTGGLACAKFVCFANAVEDNPFMAGAFHGVGEAEAVINV
                     GVSGPGVVNHAVRHAPRDLPLHDLADLIKKLSFKLARAGELVGREAARRLGLRFGIID
                     LSLAPTPLRGDSVAEIIEAFGFERAGAPGTTAALALLTDAVKKGGAMASSSVGGMSGA
                     FIPVSEDAAMIDAARVGALTLEKLEAWTSVCSVGLDMIAVPGSTSAETIAAIIADEMA
                     IGVMNSKTTAVRIIPAPGRSVGDMVEFGGLLGTAPVMPVNSFNCADFIRRGGHIPAPI
                     QSLRN"
     gene            complement(4604..>5240)
                     /locus_tag="VGB76_17230"
     CDS             complement(4604..>5240)
                     /locus_tag="VGB76_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100692.1"
                     /translation="RCLSCGASVRGRASFCPQCGSRMEGAAETRAGVEPPAQGVGSQT
                     RATAATQSNLVDEAARVAATLNDKLPTLSQEPPRTSGKGRGAPEEAHQPQAHDAGKEA
                     FDAQVLPTPLEPETRAAAEASAERGGGEAVAAGASAAQADTRGGRMRQRAASVGASMG
                     ENLRPRVEKLREASTVVFDEASDDPGLRFVIIAALLFLLFLFILLFSYILG"
BASE COUNT          940 a         1754 c         1554 g          992 t
ORIGIN      
        1 cctcggcgcc atcgccgcgc tcttcatgtt ttcatggact gcgctcttcg tgacgcttgc
       61 gctgtggtgg atttccggca gcctcggcat cggcatgggc tatcaccgcc tgctcacgca
      121 tcgcggcttc aagacgccga aatgggttga gtacgttctg acgacctgcg ccgtgctcac
      181 gctcgaaggc ggccccatcc agtggatcgg cacacaccgc atccatcacg cattcacgga
      241 aaaacccggc gacccgcact ccccgcgcga agggacatgg tgggcgcaca tgggctggat
      301 tcttacgggc acggcgcagc aacacgacga agtaacgctc cgacgttacg cgcccgacct
      361 gatgaaagac cccttccacg tctggatgtc gaaatggttt ttcgtcccgg tcgtcgcgct
      421 cggcgtgctg ctcttggtct tcggcggctg gtcggtcttc ttctggggca tgttctttcg
      481 caccactttc ggcctgcacg cgacctggct cgtcaactct gcgacccatc tctggggcac
      541 gaagcgttac aagaccgagg acgattcgac caacaactgg atcatcgccc tgttgacctt
      601 cggcgaaggc tggcacaaca accatcacgc gcacccgcgc gccgcgcgcc acggctatca
      661 ctggtatgag atcgacatga actggtacgg catccgcgcg ctccagatgc tcggcctcgc
      721 acacgacatc aaactcctcc cgcgcgaagt taaagcgcaa tccgacgagg acaatgacgc
      781 cggaagcccc gacgcgctct cgcaggcagc ttaagcagca ccgggaaaaa gataaaggtg
      841 aagggggtga gggacaaggc aatcgccttc cgttcccttg ccccttcgct tttccctccg
      901 caccctcctc ccttcgcttt gcttcttcac ctttcccctt ttcccctttt cccctttctt
      961 ctacgcttcc cctccggcct cgcgcttcct tacaatgtgc gcatgcgttt ttccggtcgc
     1021 cgacgtaaat ccatcgcctt cttcatcacg ctcggctcgt gtctggtcgc gctggccgtc
     1081 gccctgaacg tgggctggat actgctcaac tggcgcgaag tggcgttgct cgtcctcggc
     1141 atcatcttct tcagcgtcat catcgccgga ctcgtcctca acaccacttt cctcgtgcgc
     1201 gagattcgcc gcaacgaaca acacgacgct ttcatcaacg ccgttacgca cgaactcaaa
     1261 acgcccatca cctcgattcg tctctatctc gaaaccttgc agacgcgcga cgtggacgaa
     1321 caaaagcggc gcgagtttta cgccgtgatg cttgccgaca gcgaccgcct gctgcacacc
     1381 gtcgaacaag tgctgcgcgc cgggcagagc ggcggcgaca cggcgcggcg caaacgcgcg
     1441 agcgaacccg tcatcgatct cgccgcaatc gtgcgcgact cgctcgaact cgcacgcacg
     1501 cgctaccccc aactcgcggg cggcgcgttc agctacgcgg agaaattcag aaacggcgag
     1561 cgcgcactcg tgcgtggcga cgcggaggaa ctgcgcgccg tcgtctccaa cctgctcgac
     1621 aacgccgtca agtattcgcc cgacgaggtg cgcgtctcgg tgcaggtgat ggcgattgac
     1681 gcgcggcgtc tggccgtgcg cgtcagagat cagggcatcg gcattccgca ggctcaactc
     1741 aagcgcatct tcagacgttt ctatcgcgtg ccgggtcgct tgatggcgcg cttcaaaggc
     1801 actgggctgg gtctgttcat cgtgcaatcg ctcgtcaaga aacacggcgg gcgcgtctat
     1861 gccgagagcg aaggcgtggg gcgcggcagc accttcacga tcctgctgcc gcgcgcggag
     1921 agaggatgaa agcgaacgtc ctcatcgtcg aagacgaacg gcatctcgcc gacggcctgc
     1981 gcttcaacct cgaagccgag ggctacgccg tccggacaat cgccgacggc gaaaccgcgc
     2041 tcgcgctctt aacgaaccca gcggaggcgc gtttcgatgt ggtggtgctc gacgtgatgt
     2101 tgcccggaat gaacggcttc gaagtcgccg ccgaactcag gcgcgcgaat caattcgtcc
     2161 ccgtcctcat gctcacggcg cgcggtcgcc ccgaagacgt gctgcgcggc tttgaagcgg
     2221 gcgctgacga ttacctcccc aaacctttcg aactctcgat cctgctcgcg cgcctgcgcg
     2281 gcctgcttcg tcgccgcgca tggctgcatc aggaccaaga gcagaccgca gcggcggcga
     2341 cgccggacaa tcacgcgctc acaaatggcg cgacgaaaac cgctgtggat gagacgacga
     2401 aggaagcggc acaacgtgcg gcggaagagt cgttcgagtt tgacggaaag acgattgact
     2461 tcgatgcgtt ggagttacgc gtgcgggagc gcacagtgcg cctgactttg atggaggcgg
     2521 atttgctgcg tcatctcata cgccatgcgg ggcgagccgt ctcgcgcaaa tcaatgctcg
     2581 aagaggtctg gaatctgcgc gacgacacgg acacgcgcgc catcgacaac ttcatcgtgc
     2641 gcctgcgcaa gtacatcgaa gaagaacccg ccaagccacg ccatctcctg accgtgcgcg
     2701 gcgtcggcta tcgcttcatc cccaatccga aagcagcgac gagtgacaag taagaagtga
     2761 tgagtgatga gtgatgagtg atgagtaaaa acagaagtct tgtctttcac tcattactca
     2821 ttactcatca ctcatcactt cttacttgtc actgttttca gccctcaccg agccgctgat
     2881 cttcttgcgc gtcgtttcgc ccgtgtgcag cacggggagg ccgtcgtcca cctcggcgtg
     2941 cgggcgcggg atgacgtgcg tggcgacgac tttgccgacg cgcgaggctg cggctgcgcc
     3001 cgcgtcaacc gccgcgcgca ccgcccccac ctcgccgcgc acaatcgccg tcacgagtcc
     3061 ggcgtcaatc ttctcgtagc cgacgagttc cacattcgcc gctttgacca tcgcgtccgc
     3121 cgcctcgatc atcgccacga gtcccataca ttccaccatg ccgagtgctt gcatatactc
     3181 tccttagaat aacgtcatga gattctattc catgcgtctt cagtttctca aagattgaat
     3241 cggcgcgggg atgtggccgc cacgacgtat gaagtcggcg cagttgaaac tgttgacggg
     3301 catgacgggt gcggtgccga gcaggccgcc gaactccacc atgtcgccga cactccgccc
     3361 cggcgcgggg atgatgcgca cggcggtcgt cttcgagttc atcacgccaa tcgccatctc
     3421 gtcggcgatg atggcggcga tagtctcggc tgaggttgag ccgggcacag cgatcatgtc
     3481 gaggccgacg gaacacacgc tcgtccacgc ttccaacttc tccagcgtca acgcgccgac
     3541 gcgcgccgcg tcaatcatcg ccgcatcttc cgagacgggg atgaacgcgc cgctcatgcc
     3601 gcccaccgaa ctcgaagcca tcgcgccgcc cttcttcacc gcgtcggtta aaagcgcgag
     3661 tgcggcggtg gtgccgggtg cgcccgcgcg ctcgaaaccg aaggcttcga tgatctcggc
     3721 caccgaatcg cccctgagcg gagtgggcgc gagcgagaga tcgatgatgc cgaagcgcaa
     3781 gcccagacgg cgcgcggctt cgcgcccgac gagttctccg gcgcgtgcga gtttgaagga
     3841 gagttttttg atgaggtctg cgaggtcgtg cagcggcaga tcgcgcggcg catgacgcac
     3901 cgcatgattg acgacgccgg gacccgagac gccgacgttg atgacggcct cggcctcgcc
     3961 cactccatga aacgcgcccg ccatgaacgg gttgtcctcg acggcgttgg cgaaacagac
     4021 gaacttcgcg cacgccagcc cgcccgtgtc gcgcgtgagt tccgccgctt ctttgatcag
     4081 gtggccgacg cgtgcgacgg catccatgtt gattccggcg cgcgtcgtcg ccacgttgac
     4141 cgaggaacag agccgctttg tcgtcgcgag agcttccggg atcgaatcta aaaagatgtc
     4201 ttcaacgtcc gtccagcctt tttgcacgag cgcggaatag ccgccgagga agtccacgcc
     4261 gacgtgtgtg gccgccgcgt caatggcacg cgcgagcgtc agacaatccg cgaccgtgcg
     4321 cgcgcccccg gcgatgtgcg aggcgggcgt gatcgagatt cttttgttgg cgacgctgat
     4381 gccgagttcg tcgctgatct cgtccacggt ggagacgagg cgttccgccg tgcgctcgat
     4441 cttggcgcgc actcgttccg ccgtgcgttc gattgaatcg cccgcgcagt cgcgcaggga
     4501 gaggccgagc gtcacggtgc gcacgtcgag gtgctccatc tcggtcatcc gcacggtctg
     4561 caagatttcc gcttgtgaat attccataga aattgtcgcg gcctcagccg aggatataac
     4621 tgaagagcag tatgaagaga aagagcagga acagcagggc ggcgatgatc acgaaacgca
     4681 agcccggatc atccgacgct tcatcgaaca cgaccgtcga agcctcgcgc aacttctcca
     4741 cgcgcggtcg caggttctcg cccatgctcg cgccgacgct cgccgcgcgt tgtcgcatcc
     4801 gtccgccgcg cgtgtccgcc tgcgcggcgg acgctccggc ggctacggcc tcgcctcccc
     4861 cacgctccgc gctcgcttct gctgctgcgc gcgtttcggg ttcgagcggc gtgggcaaaa
     4921 cctgtgcgtc gaacgcttct ttgcccgcat cgtgcgcctg cggctggtgc gcctcttccg
     4981 gcgcgcctct ccctttgccg gaggtgcgcg gcggctcctg agagagcgtg ggcaacttat
     5041 cgttgagcgt agccgctacg cgcgccgctt cgtcaacgag attgctctgc gttgccgccg
     5101 tcgcacgcgt ttgactgccc actccctgcg ccggtggttc gacgcctgca cgagtctctg
     5161 ccgcgccctc catccggctg ccgcactgcg ggcagaaact tgccctcccg cgcacagatg
     5221 cgccgcaact caaacatcga
//
LOCUS       DASWNS010000500        10915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_228126, whole genome shotgun sequence.
ACCESSION   DASWNS010000500 DASWNS010000000
VERSION     DASWNS010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228126"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VGB76_17235"
     CDS             complement(<1..596)
                     /locus_tag="VGB76_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100693.1"
                     /translation="MSSLTITSVLAFGFVLGLKHAVEADHLAAVSTIVSERKSLLSSS
                     LVGGLWGVGHTISLLVVGLAVILFQFEISARTAQALEFCVGLMLVALGANALRKLKRG
                     GHLHLHMHQHGTRQHVHPHIHDGAPEADSHTHHGLRLSARPLLVGMVHGLAGSASLML
                     LVLSTISSPPVELLFILIFGVGSIGGMMLMSALVGLPV"
     gene            complement(1104..1673)
                     /locus_tag="VGB76_17240"
     CDS             complement(1104..1673)
                     /locus_tag="VGB76_17240"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY0100694.1"
                     /translation="MSMNEQGVVRYRIDAGRSRFFVQGTAEGMFSMFGHDPLIAIRGF
                     GGDVRCVPSTLEEASLLLLVQAGSLAVANEVSEKDRREMERGMYEDVLEIARYPEVVF
                     TSTKIAANRVGEGQYRVRIDGNLSLHGVTRVQRIDAQLAIDESGLRARGEFPLRQTDY
                     NIKPVSAVGGTLKVKDELRLAFDIVAGRA"
     gene            complement(1756..2358)
                     /locus_tag="VGB76_17245"
     CDS             complement(1756..2358)
                     /locus_tag="VGB76_17245"
                     /inference="COORDINATES: protein motif:HMM:NF018925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HEY0100695.1"
                     /translation="MRREQKRKNERGKRASEGDVLKGLAAGIVGGLVAAAVMNQFQAL
                     WGRLAEGVEGSHGAQSLQQGTPQRGVGRELQAEGKDEQTDDATERLANAVSVRVSGRE
                     LTEREKDVAGVAFHYAMGTTSGALYGAIAEFVPGVKAGAGLPFGVAVWLTADEGILPA
                     VGLSKSPTEYPLSIHVYSLSSHLIYGLTTELVRRTVRNAL"
     gene            2976..3392
                     /locus_tag="VGB76_17250"
     CDS             2976..3392
                     /locus_tag="VGB76_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY0100696.1"
                     /translation="MSMKLYVGNLSFQTSSDDLQQLFAQAGTVESASVVEDRETGRSR
                     GFGFVEMSNAEEGAKAIEQFNGKEFNGRNLTVNEARPREDRGGRGGGGGGGRGGYGGG
                     GGGGNRGGGGGYGGNRGGGGGYGGGSGGGGNREPRW"
     gene            3533..4753
                     /locus_tag="VGB76_17255"
     CDS             3533..4753
                     /locus_tag="VGB76_17255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0100697.1"
                     /translation="MTFSQLGLNDALTRACESLGYTEPTPIQEQAIPIVLSGADLIGC
                     AETGTGKTAAFLLPVIQRMSERARPGIRLLVLAPTRELATQIEASYRSLVPKKNARIV
                     TLIGGAGMARQSDALRRGVNVVVATPGRLLDHMERGAIDFSFVETLVLDEADRMLDMG
                     FWPAIRRVLAALPTERQTLLFSATMSSAIEKIARSNMRQPKLVEVSQRGAAAVTVEQT
                     AYPVATESKTALLLDLLERERFERVLVFTRTRRGAERLSHILEARSHKVNRIHADRTQ
                     PQREAALRGFKDGQTRVLVATDIAARGIDVDSISHVINYDVPAAPEDYVHRIGRTGRA
                     GNRGRAITLVTPVEELNMRAIERLTGQAIERILLQGFGGVKPAATNTIKPFAGVKRAG
                     SSVGRRSFRPRRSR"
     gene            4908..5294
                     /locus_tag="VGB76_17260"
     CDS             4908..5294
                     /locus_tag="VGB76_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100698.1"
                     /translation="MIENSETVATIEKIDKRTFEVGERVEKLCTKCEVERGHVVASVT
                     KRGQVSRVTCPICQTRSTFKSGVKTSGSRSSQAGAPYDPSRQYRAGQTLMHPAFGLGE
                     VTALIEPQKIDVLFADRMRRMIHGRA"
     gene            complement(5440..6828)
                     /locus_tag="VGB76_17265"
     CDS             complement(5440..6828)
                     /locus_tag="VGB76_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100699.1"
                     /translation="MKKLLQTISLALLLLCLGSAEAARADVKIKTKNTSAGHTSEGVT
                     YIKGKRQRNEQGETSVTITQCDLRRNVQLGVPTKTYVLMPFDQKPDANGATGSAAKPS
                     PAATAVRRGGIVTSTFTSTDTGERKKMFGYTARRIKTSIQTESSPDACQVNRSRMETD
                     GWYIDAQFALDCDYGTSAGYVPPTPAGGCRDEYRMKQTGVAKLGYPVSVTTTLFDDKG
                     QPSFTFSQEVVEITNAVLDSALFEVPADYRQVNDMSEMYSAAAMTAASGNSSAADDDE
                     NASNNSGAAKMNSTVGAKRAGVVRVGVAMTETGVMGENVDAAVLAQSVRRALMSQLGN
                     ANVEVVALDASGARALESEATQKECDFVLQSTVAHKKGGGGGFGGFLKKSAAIASGTV
                     APISNTGIAVAANSLKAKDELTLEYSLQRGAEPVVANTLKAKAKSDGDDLVSQLAGQA
                     AVAVRAAVATKK"
     gene            7141..7710
                     /locus_tag="VGB76_17270"
     CDS             7141..7710
                     /locus_tag="VGB76_17270"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0100700.1"
                     /translation="MTTPSPQDVTRLLIQLTDGNRTVLDELLPLVYGELRSLAANYLR
                     RERSSHTLQPTALVHEAYLRLVDQNQVRWQNRAHFFGVAAQMMRRILVDHARGHNADK
                     RGGEIHKVSLDENIDVTDERATDLIALDDALTALAEIDPDKARLVELRFFGGLSVEET
                     AEVLGVSAPTVKRQWRMAKAWLYGQVQKQ"
     gene            7801..>10915
                     /locus_tag="VGB76_17275"
     CDS             7801..>10915
                     /locus_tag="VGB76_17275"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0100701.1"
                     /translation="MTPERWKQVEEVFQAALDLPRDERPKFIGEASAHDDALRKQVEA
                     LVAQYEEAGDFIEEPAFAVEGFNPGSAPFRTTQPTDLENDPMAGARVGAYKIVREIGR
                     GGMGAVYLAVRADSEYQKRVAIKLVKRGMDTDFILRRFRNERQILASLDHTNIARLLD
                     GGTTETGLPYFVMEYIEGQPLYRYCDSEKLTIAERLRLFVQICHAVAYAHQNLVVHRD
                     IKPSNIIICSDGTPKLLDFGIAKLLNPELSPDTITPTATAMRLMTPEYASPEQVRGEQ
                     ATPASDIYSMGVLLYELLTAHRPYHFPSRSPHEIARVICEEEPEHPSRVVSRNEGLIP
                     LDVASSGEATLEFICKMRGGTLDSVRRELAGDLDNITLKALRKEPGRRYTSVERLRED
                     IVRHLEGRPVSAPFVFPSAAPKLSRLATGEAVTGEKTIAVLPFKLLNIQRTEETGENY
                     LGIGLADALITRLSNVRRFSVRPTSSVIRYGDGQTDPFASGHELGVSYVIDGRIQRAR
                     DRIRITVQLLNVYEGSTMWAGQFDEKFTDVLSLEDAISAQVAQALVPQLTGDEREQLA
                     KRGTDNAEAFEVYLRGRYHWNTFTEEGFAKAITCYYRAIALDPDYAAAYTGIADYYNW
                     IGIYGVMPPAECSAAAKEAGSRALELDESLPDAHTAFGFALHTHDLNWESPIAHYERA
                     LELNPHHASSYLWHGTQLMMEGNFDAGLPLVRRSIELDPLTLFNQHMLGFCLFYARRL
                     DESLSVYRELVETVPTHGLAYFGYSWTLSQLGRHDEAIDAAVKAIEISGPSPFITATL
                     AYAYAAAGRKQEAHHVLGELEELSAKRYVSPYYRAIAHCALGDNKRAFTLLEQSFADR
                     EAWVVWLGIQPQFDPLRSDPRYEELLRRTKNPGIARLRGESSPASGRASGALSGQGSS
                     AELARQQSTDARHLTDNEEARQLYVAGRYFSTRRTADGLRQAIKRFERAVELDPKFAM
                     AYAELADCYALLNWYVEPPPAGAFEQAKQAALKAVEADPNLAEAHASLGYIKLHYEHD
                     WTGAEQEF"
BASE COUNT         2108 a         3544 c         3195 g         2068 t
ORIGIN      
        1 tagacgggca ggccgaccag ggcgctcatc aacatcatgc cgccgattga gccgacaccg
       61 aaaataagga taaagagaag ttcgacgggc ggcgaggaga tcgtggagag caccagcagc
      121 atcaacgacg cgctcccggc caacccgtga accattccga cgagcaaggg tcgtgcgctg
      181 agccgcaggc cgtggtgcgt atgcgaatct gcttcgggtg cgccgtcgtg gatgtgcggg
      241 tggacgtgct ggcgtgtgcc gtgctggtgc atatgtaagt gtagatggcc gccccgtttc
      301 agtttccgca gggcattggc tccgagtgcg accaacatga ggccgacgca gaactcaagc
      361 gcttgcgccg tccgcgcgct gatctcgaac tggaagagaa tgacggcgag gccgacgacg
      421 agcagcgaga tggtatggcc gacgccccag aggccgccga cgagcgacga actcaagagg
      481 ctcttgcgct cgctgacaat cgtggagacg gctgccaggt ggtcggcttc gaccgcatgt
      541 ttcaagccca gcacgaagcc gaaagcaagc actgaagtga tagtgagcga actcatccga
      601 agtaatcgag aatcttgaaa tggcgtcccg gtctagtttg cgcccgcgtg atttatggcc
      661 gtcacgcagg cgcgagccga aaacttttgc gctgaactct agataactat agcactaaca
      721 gattcccggc aactgttcgc caacgagcat atccacgatg cgcgtcccgc cgaagcccgt
      781 ccgcatcgcc acgatgccct gtggctcagg cttagcctca cagatgatgc atggacagga
      841 acttcctcga tgaccagttg cgaaccttcc agcgcagttc cctgacacgc cacttcataa
      901 gagaacagga gggcatcttt gaacacgctc gacagttgcc ccggtttgag atgaatcgca
      961 aaaaccctga cgcctccgca acgcgcaagc ctcttcctcc gccatctgca ccatactcat
     1021 agctatcgaa agctcttgca tagctcgtga atcgtaaatc gtgaatagaa gaagatggat
     1081 tgaacctatt tacgatttac gcttcacgct ctaccggcaa cgatgtcgaa cgcgagtcgc
     1141 agttcatcct tgaccttgag cgtgccgccg acggcggaga cgggcttgat gttgtaatcg
     1201 gtctgtcgca aaggaaactc gccacgcgcc cgcaggccgc tttcatcgat agccaactgt
     1261 gcgtcgatcc tctgaacgcg cgtgacgccg tgcagcgaca agtttccgtc gatcctgaca
     1321 cggtactgcc cttcacccac ccggttcgca gcgatcttcg tgctcgtgaa gacgacctcc
     1381 gggtagcggg cgatttcgag cacatcttca tacatcccgc gctccatctc tcgcctgtcc
     1441 ttctcgctta cttcgttggc gacggcgagc gaacccgcct gcaccagcag cagcagcgac
     1501 gcctcttcga gcgtgctggg gacgcatcgc acgtcgccgc cgaagccgcg tatcgcgatc
     1561 aacggatcgt ggccgaacat cgagaacatg ccctccgccg tcccctgcac gaagaagcgg
     1621 ctccgcccgg catctatgcg atagcgaacc acgccctgtt cattcatgct cataagttcc
     1681 gtttgagaca ggcgcacgtc cctccgctcc gtgcccggcg aaacgtttcc ggcgtccgat
     1741 gcctgtctcc gctcgtcaca gtgcgttgcg gacggtgcgc cgcacgagtt ccgtcgtcag
     1801 cccgtagatc aagtgcgacg agagcgaata gacgtggatc gagagcggat attccgtggg
     1861 cgactttgac aggccgaccg caggcaggat gccctcgtcc gccgtgagcc acacggccac
     1921 gccgaagggc aagcccgcgc ccgccttcac tcccggcaca aactccgcga ttgcgccata
     1981 aagcgcgccc gatgtcgtcc ccatcgcata atgaaaggcg acgcccgcga catctttctc
     2041 gcgctccgtc aactcgcgcc cggagacgcg gacagagacg gcgttggcga gtctctcggt
     2101 cgcgtcatcc gtctgttcat cttttccctc ggcttgcagt tcgcgcccca cgcctctctg
     2161 cggcgtgccc tgctgcaagg attgcgcccc gtgcgatccc tccacgcctt cggccagcct
     2221 gccccagagg gcttgaaatt ggttcatcac cgccgccgca accagcccgc cgacgatgcc
     2281 tgccgccaat cccttcaaga catcgccttc gctggcgcgc ttcccgcgtt cgttttttcg
     2341 tttctgttct cttctcatca catcctactg atcttgatat agcctttaat gtcgggcaga
     2401 ctcacgacga ctgtggcggc gagtgccaac gccgccactc ccacgattga ccacaagtta
     2461 attccggtcg gcctccggct ccttactctc taatgattga ttatatttca tgacaactca
     2521 acttcctttc tcctctaacc acatgagttt aacctgccgg atactttttg cctcgttctc
     2581 atccgtgtca atagtttcag gcttccgggg aatgttctaa atgtgtggca tcgcacaagg
     2641 ccgcccggag ggttgatgaa acggctcgga gtcagcagcc ggaatcagga cgttggcagg
     2701 cggtcgaagt cgcgcagagc ttgcaactta agggcttatt agtgcatcat agtggttggc
     2761 gaattccttc tctctggcat caacggtgat gcgccccgcg ctagataagc ttttacagcg
     2821 gcgtttcgga tgattcgaga gtgggttgat ttcaacttta gaagcgctcc cttgagcacc
     2881 cgaacgcaag ccttcgctgc ctaccgttac atcgaagcca ctttgattcg acagcttctc
     2941 tcagatactc ctacccatag gagaggtaca tctacatgtc tatgaaactt tacgtcggca
     3001 acttgtcgtt ccaaacttcg agcgacgact tgcaacaatt gttcgcgcag gcgggcacag
     3061 tcgaatcagc ttcagtggtt gaagatcgcg aaacgggacg ctcacgcggt ttcggcttcg
     3121 tcgagatgtc gaacgcggaa gaaggggcga aagccatcga gcagttcaat ggcaaggaat
     3181 tcaacggtcg caacctgacc gtcaacgaag cgcgcccgcg cgaagatcgc ggcggacgtg
     3241 gcggtggcgg cggcggtggt cgcggcggct acggtggtgg cggcggcggt ggtaatcgcg
     3301 gcggcggtgg cggctacggc ggcaaccgtg gcggcggcgg tggctacggc ggcggcagcg
     3361 gtggcggtgg caaccgcgaa ccgcgctggt aagctcccgg cacactcgac cggtgactct
     3421 ttaagtaaag cgcctccgac gatgaactaa cgccgccgga ggcgctttca atttttcagc
     3481 taacaagagg tgcgctccca tatgaatggt cgcagccgcg ccaaaggatt ttatgacttt
     3541 ttctcaactg ggactcaacg atgcgctcac gcgcgcctgt gagtctctcg gatacaccga
     3601 accgacacca attcaagagc aggccatccc catcgtcctc tccggtgccg atttaatcgg
     3661 ctgcgcggag acaggaacgg gcaagaccgc cgcgtttctg ctgcccgtaa tccaacgcat
     3721 gtcggagcgc gcacgccccg gcatacgcct gctcgtcctt gcgccgacgc gcgaactggc
     3781 gacgcagatc gaagccagct atcgctcgct cgtgccgaag aagaacgcgc gcatcgtcac
     3841 cttgattggc ggcgcaggca tggcgcgcca gagcgacgcg ctgcgtcgcg gcgtaaacgt
     3901 cgttgtcgcc acgcccggac gcctgctcga ccacatggag cgtggcgcaa ttgatttctc
     3961 ttttgtcgaa acgctcgtgc tcgacgaagc cgaccggatg ctcgacatgg gcttctggcc
     4021 tgcgattcgt cgcgtgctgg ccgcgctgcc caccgaacgg cagacgcttc tcttctccgc
     4081 cacgatgtct tcggccatcg agaagatcgc gcgctcgaac atgcgccaac cgaaactcgt
     4141 cgaagtcagc cagcgcggcg cagccgccgt cacggttgaa cagaccgctt acccggtggc
     4201 gaccgaatcg aagaccgcgc tcctgctcga tcttctggag cgcgaacgct ttgagcgcgt
     4261 gctcgtgttc acgcgcacgc gacgcggcgc ggaacgcctc tcgcacatcc tcgaagcgcg
     4321 ttcgcacaaa gtgaaccgca tccacgcaga ccgcacccaa ccgcagcgcg aagcagccct
     4381 gcgcggcttc aaagacggcc agacgcgtgt gctcgtggca accgacatcg ccgcgcgcgg
     4441 cattgacgtt gactcgatct cgcacgtcat caattacgac gtgcccgccg cgccggaaga
     4501 ctacgtgcac cgcatcggtc gcacgggtcg cgccggaaat cgcggacgcg ccatcacgct
     4561 cgttacgccg gttgaagaat taaacatgcg cgccatcgag cgtctgaccg ggcaggccat
     4621 cgagcgcatc ctgttgcagg gtttcggcgg cgtgaagcct gctgcgacga atacgatcaa
     4681 gccgtttgcc ggagtcaaac gcgcgggcag cagcgtcggt cgtcgctctt ttcgcccgcg
     4741 tcgctccaga taactacggc gcgccggatg aactccggtt tcgccggata acacggcagg
     4801 caggcgaacg tcgcggcaaa agcgttcgtc gaggcactgc tgagaatagc cagtaaccgc
     4861 gcttctgccc gcccgcgatg cagaggcagc tttgaaaggt aacgcgcatg attgagaact
     4921 ctgagacggt tgcgacaatt gagaagatcg acaagagaac gtttgaggtc ggcgagcggg
     4981 tggagaagct ctgcaccaaa tgtgaagtcg agcgcggcca cgtcgtcgcg tccgtcacga
     5041 agcgcggtca ggtgtcgcgc gtaacgtgcc cgatctgcca gacgcgcagc acgttcaaga
     5101 gcggcgtcaa gacttccggc tcgcgctcgt cgcaagcggg cgcgccttac gatccgtcgc
     5161 gccagtatcg cgcgggacag acgttgatgc acccggcctt cggcctcggc gaagtcaccg
     5221 cgctcatcga accgcagaag atagacgtgc tctttgccga ccgcatgcgg cggatgattc
     5281 acggtcgcgc ctgaaaaaaa gataaaagag acgtagaggt gtttgacctc cacgtctctt
     5341 tccggctctc cctgcccaaa ggaagaggtc tgttgcgcga cgaccgacgc ccgtgcggct
     5401 gtggtcttcg gcgcgtttac ttcgtcatct ctgccgcgct cacttcttcg tcgctacggc
     5461 ggcgcgcacc gcaaccgcag cctgccccgc gagttgcgac accaaatcat cgccgtcgga
     5521 cttcgccttg gccttcaaag tgttggcgac gaccggctcc gccccgcgct gcaagctgta
     5581 ttcaagcgtc aactcgtctt tggctttcag agaatttgcc gccacggcta tgccggtatt
     5641 gctgatggga gctaccgtgc cgctggcgat ggccgcagac ttcttgagga aaccgccgaa
     5701 gccgcctccg ccgcctttct tgtgcgcgac cgtgctctgg agcacgaagt cgcactcctt
     5761 ctgcgtcgcc tccgactcca aagcccgcgc cccgctcgcg tcgagcgcga cgacctcgac
     5821 gttcgcattg cccaactggc tcatcaacgc gcggcgcacc gattgagcga gcaccgccgc
     5881 atccacgttt tcgcccatca ctcccgtctc cgtcatcgcc acgccgacgc gcaccacgcc
     5941 tgctctcttc gcgccgaccg tcgagttcat cttcgccgcg ccgctgttgt tcgaagcatt
     6001 ctcgtcatcg tcggcagcgc tcgaattgcc ggatgccgcc gtcatggccg ccgcgctgta
     6061 catctcgctc atgtcgttca cctgacgata atcggcgggc acttcgaaga gcgcgctgtc
     6121 gagcacggcg ttcgtgatct ccaccacctc ctgcgagaag gtgaaggacg gctgcccctt
     6181 gtcgtcgaag agggtcgtcg tgaccgagac gggatagccg agttttgcca cgcccgtctg
     6241 tttcatgcgg tattcgtcgc ggcagccgcc cgccggagtc ggtggcacgt agcccgcgct
     6301 cgtcccgtag tcgcagtcga gcgcaaactg cgcgtctatg taccagccgt ccgtctccat
     6361 gcggctgcgg ttgacctggc aggcatccgg cgacgactcg gtctggatgg aagtcttgat
     6421 gcggcgtgcc gtgtagccga acattttctt gcgctcgccc gtgtccgtgg aggtgaaggt
     6481 cgaggtcacg atgccgccgc gtcgcaccgc agttgcggcg ggcgacggtt tcgccgcgct
     6541 gcccgtcgcg ccgttcgcat ccggtttctg gtcgaagggc atgagcacgt aggtcttcgt
     6601 cgggacgccg agttgaacgt ttcttctcag gtcgcactgg gtaatcgtta cggaagtttc
     6661 gccctgctcg ttgcgctggc gtttgccctt gatgtaggtc acgccttcgg aggtgtgccc
     6721 ggcggaggtg ttcttggttt taattttaac gtcggcgcgt gcggcctccg cgctgccgag
     6781 acagagcagg aggagcgcga gactgattgt ttgcagtagt tttttcatgt tagcgattgt
     6841 ccctctatgg ctgaattaac gtgaagccgg acggaagacg cgcgcgcctc tgagcgtgcg
     6901 agaaattgtt cggctcttac tatattacgc gtaacgaaat ccttaaagag atcatcaaag
     6961 aggtttgcgg attgcggcgg cgggtcgagg ctcgggcggg cgggtcgggt tatttaaatc
     7021 agacttgagg cacaaacagg gcgcgcgaat tgataagcct ccggcagtcg gtttatactt
     7081 gcgttgctgc atgtcggtaa taatggaaag cgtatagcta cggatgccgg gaattcccct
     7141 gtgacaacac cctcgcctca agacgtaacc cgtttgctca tccagttgac ggatggcaac
     7201 cggactgtgc tggacgaact gctgccgctc gtctatggcg agttgcgcag tttggccgcc
     7261 aactacctgc gccgcgaacg ctcctcgcac acgctgcaac ccacggcttt agtgcacgaa
     7321 gcctacctgc ggctcgtcga tcaaaatcag gtcaggtggc aaaaccgcgc gcacttcttc
     7381 ggcgtcgccg cgcagatgat gcgccgcatc ctcgtggatc acgcgcgcgg acataacgcc
     7441 gacaagcgcg gcggcgaaat tcacaaagtc tcactcgacg agaacatcga cgtgacggac
     7501 gaacgcgcga ccgacctcat tgcgctcgac gatgcgctca cggctctcgc cgagatcgac
     7561 ccggacaaag cccgcctcgt cgaactgcgt ttcttcggcg gcctctccgt ggaagagacg
     7621 gcggaagtgt tgggcgtcag cgcgccgacg gtcaaacgcc agtggcgcat ggcgaaggca
     7681 tggctctacg gacaagtaca aaagcagtga cgagtgagag gtgacgagtg acaagttaaa
     7741 acccgaaact tgtcctgatt cctgctcgtc acctgccact cgtcactcat cactgttttt
     7801 atgacccccg aacgttggaa gcaagtagaa gaggtttttc aagccgcgct cgatctcccc
     7861 cgcgatgaac gccccaagtt catcggggag gccagcgcgc acgacgacgc tttgcgcaaa
     7921 caggtcgaag ctctcgtcgc gcagtatgaa gaggccgggg atttcatcga ggaaccggcc
     7981 ttcgccgtcg agggcttcaa tcccggtagc gcgcccttcc gcaccaccca acccaccgat
     8041 cttgagaacg acccgatggc gggcgcacgc gtcggcgcgt acaagatcgt gcgcgagatc
     8101 gggcgcggcg gcatgggcgc ggtctatctg gccgtgcgcg ccgacagcga ataccagaaa
     8161 cgcgtcgcca tcaaactcgt caagcgcggc atggacaccg atttcatcct gcgccgcttc
     8221 cgcaacgaac gacagatttt agcctcgctc gatcacacca acatcgcgcg tctcctcgac
     8281 ggcggcacga ccgaaaccgg cctgccctac ttcgtcatgg agtacatcga agggcagccg
     8341 ctctatcgct actgcgacag cgagaagctg acgattgcgg aacgcctccg cctcttcgtg
     8401 cagatctgcc acgccgtcgc ctacgcgcat caaaatctcg tcgtccatcg cgacatcaaa
     8461 ccgtcgaaca tcatcatctg ttccgacggc acgccgaagc tgttggactt cggcatcgcc
     8521 aagctcttaa accccgaact ctcgcccgac accatcacgc cgacggcgac cgccatgcgc
     8581 ctgatgacgc ccgaatacgc cagccccgaa caggtgcgcg gcgaacaggc caccccggcc
     8641 agcgacatct acagcatggg cgtgctgctc tacgaacttt taaccgcgca tcgcccttat
     8701 cacttcccgt ctcgttcgcc gcacgagatc gcgcgcgtca tctgcgagga agaaccggag
     8761 caccccagcc gcgtcgtctc gcgcaacgaa gggctgatcc cgctcgacgt ggcgagcagc
     8821 ggcgaggcga cgcttgagtt tatctgcaag atgcgcggcg gcactctgga cagcgtgcgc
     8881 cgcgagttgg cgggcgacct cgacaacatc accttgaagg ctctgcgcaa agagccgggc
     8941 aggcgttaca cgtcggtcga gcggctgcgc gaagacatcg tgcgccatct ggaagggcgt
     9001 cccgtctccg cgccgttcgt cttcccttcc gcggcgccga aactttcgcg gctggcgacg
     9061 ggcgaggccg tcacgggcga aaagacgatt gccgtgctgc ccttcaaact cctcaacatc
     9121 caacggacgg aggagacggg cgaaaactat ctcggcatcg gcttggccga cgcgctcatc
     9181 acgcgcctgt cgaacgtgcg ccgcttcagc gtgcgcccga cgagttccgt catacgctac
     9241 ggcgacgggc agaccgatcc cttcgcctcc ggccacgaac tcggcgtcag ctacgtcatc
     9301 gacgggcgca tccagcgcgc gcgcgaccgc atacgcatca ccgtccagtt gctcaacgtc
     9361 tacgaaggct cgacgatgtg ggcgggtcag ttcgatgaaa agttcactga cgttttaagt
     9421 ctggaagacg ccatctcggc gcaggtcgcg caggcgctcg tgccgcaact gacgggcgac
     9481 gagcgcgagc agttggcgaa gcgcggcacg gacaacgcgg aggcgtttga agtctacctg
     9541 cgcggtcgtt atcactggaa cactttcacc gaagagggct tcgccaaagc catcacctgc
     9601 tactaccgcg ccatcgcgct cgaccccgac tacgccgccg cctacacggg catcgccgat
     9661 tactacaact ggatcggcat ctacggcgtg atgccgcccg cagagtgctc cgccgccgcg
     9721 aaggaagcag gctcgcgcgc gcttgaattg gacgagtcgc tccccgacgc gcacacggcc
     9781 ttcggcttcg ccctccacac gcacgatctc aactgggagt cgcccatcgc gcactacgag
     9841 cgcgcgctcg aactgaaccc gcaccacgcc tcctcctatc tctggcacgg cactcaactc
     9901 atgatggagg gcaacttcga cgcggggctg ccgctcgtgc gtcgttcgat agaactcgac
     9961 ccgctcacgc tcttcaatca gcacatgctg gggttctgcc tgttctacgc gcgccgcctt
    10021 gatgaaagtc tctccgtcta tcgcgagctg gttgaaaccg tgccgacgca cggcctcgcc
    10081 tatttcggtt atagctggac gctcagccaa ctcggcaggc acgacgaagc gattgacgcg
    10141 gcggtgaaag ccatcgaaat ctccggaccc agccctttca tcacggccac gctcgcttac
    10201 gcttacgccg ccgccgggcg caagcaggaa gcgcaccacg tcctcggcga gttggaagaa
    10261 ctctccgcga agcgttacgt ctcgccctat tatcgcgcga tagcccactg cgcgcttggc
    10321 gacaacaagc gcgccttcac cctcctcgaa cagagtttcg ccgaccgtga agcgtgggtc
    10381 gtgtggctcg gaatccaacc gcagttcgat ccgctgcgtt ccgatccgcg ttacgaagag
    10441 cttttacgcc ggacgaagaa tccgggcatc gcgcgtctgc gcggcgagtc ctcgcctgcg
    10501 tcggggcgtg cgtcgggcgc gctctccggt cagggcagca gcgcggaact cgcccgccag
    10561 cagagcacgg acgcgcgcca tctcaccgac aacgaagaag cgcgccaact ctacgtcgcc
    10621 ggtcgctact tctccacgcg ccgcaccgcc gacgggttgc gacaggccat caagcgtttc
    10681 gaacgcgccg tcgaactcga ccccaagttt gcgatggctt atgccgaact cgcggattgt
    10741 tatgccctct tgaactggta cgtcgagccg ccgcccgcgg gcgcgttcga gcaggcgaag
    10801 caggccgcgc tcaaggccgt ggaggccgac ccgaatctgg ccgaagcgca cgcctcgctc
    10861 ggctatatca agctccacta cgaacacgat tggacgggcg cggaacagga gtttc
//
LOCUS       DASWNS010000501         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_70602, whole genome shotgun sequence.
ACCESSION   DASWNS010000501 DASWNS010000000
VERSION     DASWNS010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70602"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VGB76_17280"
     CDS             <1..197
                     /locus_tag="VGB76_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100702.1"
                     /translation="GSSRVVVMRDGQRVGELRGDEISQSNIISAMAEGTGGEATAAST
                     ATAHVTDTNPPAAEPPSKGA"
     gene            203..1204
                     /locus_tag="VGB76_17285"
     CDS             203..1204
                     /locus_tag="VGB76_17285"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0100703.1"
                     /translation="MQNATPANSSSSSGGTQARSAGASRTLLADYGPTYGALAALVLL
                     VLANALFTPNFADVNNFRNILLQVAPTVLVAVGMTLVIATGGIDLSVGSVMAIAAALA
                     STNLERGLFPAVALGLLAALAVGLFNGALISGFRIQPIIVTLAMLITGRGVAQVVSRG
                     GQIILIEDQNFMSLGKGFVGPVPMSVVVMLLVVAVAIFVMRATSFGRYVAAVGGNEAA
                     ARLAGIKVHRTKITVYALSGLLAGLAGLIEAARSEASDATKVGLYIELDAIAAVVVGG
                     TPLTGGRVTVVGTLIGALIMQVITTSFNMLGIGFAWSLVLKAAIILIAVYIQRPKVA"
     gene            1215..2750
                     /locus_tag="VGB76_17290"
     CDS             1215..2750
                     /locus_tag="VGB76_17290"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0100704.1"
                     /translation="MKKQLCAFVVLVVLVQAMPAPPSVRAQTTKRTVESGKRYARLVV
                     RNATVIDGNGTPAAGPFDIVVEGNTITEIVGLDPVAVKSGTARRPAGDVEIDATGKYV
                     LPGLINAHGHVQEERGGVAQPLDYELKLWLACGITTVRDVGSNVPKTLALKRASAAGE
                     VAAPRLFVYAVYAAGGAPKTAEAARQRVRDLKAQGVDGLKMFAMDRDLMAAAWDEARK
                     NGLRIAHHAAIAETNAWDDIKFGTTSIEHWYGIPDAAIEAGVQNFPPAYNYNNEVDRF
                     RHAGRLWREANWDKLMKVLDAMAEARVAWDTTLVIYEASRDLQRAQTQPWFADYLHPT
                     LEDYFRPDPANHGSYFIGWTSTDEAFWKENYRIWMAALREFERRGGVIGVGEDAGFIY
                     QMYGFGLLRELELHEEAGFHPLKVIQHATGNNAKILGQEDRLGRVRVGYAADLLVVNG
                     NPLENLKVLYPVGVETIQDGRAVRTGGIEWTIKDGIPYHAPTLFAGVKEIVARARAER
                     RKK"
     gene            2810..>3451
                     /locus_tag="VGB76_17295"
     CDS             2810..>3451
                     /locus_tag="VGB76_17295"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0100705.1"
                     /translation="MSQAATTIDEEGSAHGRLMRARFATFVQRQGALIALVAVCVFAA
                     IRYERFLTEENLVNVLRQNSMLGLVALGMTFVILTGGIDLSVGSLMAVAGVAAATLAG
                     RGVLVAVVAAVAITTALGLVNGLVIAKARIQPFIVTLAMMIAARGLVLAYTGERSIGI
                     GKSSESLTSLGRDFIGPVPVPVVFLIVAFALGWLVQNHTRFGRHVYAIGDNDEA"
BASE COUNT          616 a         1047 c         1181 g          607 t
ORIGIN      
        1 agggcagttc gcgcgtcgtg gtgatgcgcg acgggcagcg cgtgggcgaa ctgcgcggcg
       61 acgaaatctc acaatccaac atcatcagtg cgatggccga aggcacgggc ggcgaggcga
      121 ccgccgcttc aaccgcgacc gcacatgtga cggacaccaa cccacccgcc gccgagccgc
      181 cgtcaaaagg agcctagaga agatgcagaa cgccacaccg gccaacagca gcagcagcag
      241 cggcggcacg caagccagaa gcgcgggcgc aagccgcacg ctcctcgcag actacggccc
      301 gacttacggc gcgctcgccg cgctcgtcct gctcgttctg gcgaacgctc tgttcacgcc
      361 gaacttcgca gacgttaaca acttccgcaa catcctcctg caagtcgcgc cgaccgtgct
      421 ggtcgccgtc ggcatgacgc tcgtaatcgc gacgggcggc atcgatctct ccgtcggttc
      481 ggtgatggcg attgccgccg cgctcgcctc cacgaatctc gaacgcggcc tcttcccggc
      541 ggtcgcgctc ggacttctgg ccgcgctcgc cgtcggactt tttaacggcg cgctcatctc
      601 cggttttcgc atccagccga tcatcgtcac gctcgccatg ttgatcacgg ggcgcggcgt
      661 ggcacaggtc gtcagtcgcg gcgggcagat catcttgatc gaggatcaaa atttcatgtc
      721 gctcggcaaa ggcttcgtgg ggcctgtgcc gatgtcggtc gtcgtgatgc tcttggtggt
      781 cgccgtcgcg atcttcgtga tgcgcgccac gagtttcgga cgttacgtgg cggcggtggg
      841 cggcaacgaa gcggcggcgc ggctcgccgg gatcaaagtc caccggacga agatcacggt
      901 ctacgctttg agcggcctgc tcgcgggact cgcgggactt atcgaagcgg cgagatcgga
      961 agcgagcgac gccacgaagg tcggactcta catcgaactc gacgcgattg cggcggtcgt
     1021 cgtcggcggc acgccgctga cgggcgggcg cgtgacggtc gtgggcacgc tcatcggcgc
     1081 gctcatcatg caggtcatca cgacgagttt caatatgctc ggcatcggct tcgcgtggtc
     1141 gctcgtcctg aaggcggcca tcattctcat cgcggtctac attcaacgcc cgaaagtggc
     1201 ctgacctctc ccgaatgaaa aaacaactct gtgcctttgt cgtccttgtc gtgctcgtcc
     1261 aagcgatgcc tgccccgccc tccgttcgtg cgcaaacaac gaaacggacg gttgagagcg
     1321 gcaagcgtta cgcgcgtctg gtggttcgca atgcgacggt gattgacggg aacggaacgc
     1381 ccgctgcggg accgttcgat attgtcgtcg agggcaacac gatcacggag atcgtcgggc
     1441 ttgatccggt ggcggtaaag agcggcacgg cgcggcgacc cgcaggggat gtggagattg
     1501 atgcgacggg caagtatgtg ttgcccggtt tgatcaacgc gcacgggcat gtgcaggagg
     1561 agcgcggggg cgtcgcgcag ccgctcgact acgagttgaa actgtggctc gcctgcggca
     1621 tcacgacggt gcgcgacgtg gggagcaacg tgccgaagac gctcgccttg aagcgtgcga
     1681 gcgcggcggg cgaagtggcc gcgccgcgcc tcttcgtcta tgcggtctac gcggcgggcg
     1741 gcgcaccgaa gactgcggaa gcggcgcgcc agcgcgtgcg ggatttgaag gcgcagggcg
     1801 tggacggcct caagatgttt gcgatggatc gcgacctgat ggcggcggcg tgggacgagg
     1861 cgcggaaaaa tggtctgcgc atcgcgcatc acgcggccat cgccgagacg aacgcctggg
     1921 acgacatcaa gttcggcacg acctcgattg aacactggta cggcatccct gacgcggcga
     1981 ttgaggcggg cgtgcagaat tttccgcccg cttacaacta caacaacgaa gtggatcgct
     2041 ttcgccacgc ggggcggctg tggcgcgagg cgaactggga caagttgatg aaggtgctcg
     2101 acgcgatggc cgaagcgcgc gtcgcgtggg acaccacgct cgtcatctac gaggcgagcc
     2161 gcgatttgca gcgcgcgcag acgcagccct ggttcgctga ctacctgcac ccgacgctcg
     2221 aagactactt tcgtcccgac cctgcgaatc acggctcata cttcatcggc tggacttcga
     2281 ccgacgaggc tttctggaaa gagaactacc gcatctggat ggctgccctg cgcgagtttg
     2341 agcgacgcgg cggcgtgatc ggcgtgggcg aagacgcggg cttcatctac cagatgtacg
     2401 gcttcggtct gctgcgcgag ttggagttgc acgaggaggc gggctttcac ccgctcaaag
     2461 ttatacagca tgcgacgggc aacaacgcga agattctcgg acaggaagat cgtctggggc
     2521 gcgtgcgcgt cggctacgcg gcagacttgc tggtggtgaa cggtaacccg ctcgaaaatt
     2581 tgaaagtgct ctatcccgtg ggcgtggaga cgattcagga cgggcgcgcg gtgcgcacgg
     2641 gcggcattga gtggacgatc aaagacggca tcccatatca cgcgcccacg ctgtttgccg
     2701 gagtcaaaga gatcgtggcg cgcgcgcgtg cggagcggcg aaagaagtag ctgcgtcgag
     2761 ataaacgccg gggcgacatt caacacgaag tttaaagacg gagaagatta tgagccaggc
     2821 agcgacgaca attgacgaag aaggttcggc gcacgggcgt ctgatgcggg cgcggttcgc
     2881 cacgttcgtg cagcgacagg gcgcgctcat cgcgctcgtg gcggtctgcg tgttcgccgc
     2941 gattcgttac gagcgtttcc tgacggaaga gaatctggtg aacgtgctgc gccagaacag
     3001 catgctcgga ctcgtcgcgc tcgggatgac cttcgtgatc ctgacgggcg gcatagacct
     3061 ctcggtcggg tcgctgatgg ctgtggcggg cgtggcggcg gcgacgctcg cggggcgcgg
     3121 cgtgctcgtc gccgtcgtcg cggcggtcgc catcacgacc gcgctcggcc tcgtcaacgg
     3181 cctcgtcatc gcgaaggcgc gcatccagcc gttcatcgtg acactggcga tgatgattgc
     3241 ggcgcggggg ctggtgctgg cctacacggg ggagcggtct atcggcatcg gtaagagttc
     3301 cgagtcgctc acgtctctgg ggcgcgactt catcggaccc gtgcccgtgc ccgtcgtctt
     3361 cctcatcgtc gccttcgcgc tcggctggct cgtccagaat cacacgcgct tcgggcggca
     3421 cgtctatgcg attggcgaca acgacgaagc g
//
LOCUS       DASWNS010000502         7199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_408034, whole genome shotgun sequence.
ACCESSION   DASWNS010000502 DASWNS010000000
VERSION     DASWNS010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408034"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..1361
                     /locus_tag="VGB76_17300"
     CDS             348..1361
                     /locus_tag="VGB76_17300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0100706.1"
                     /translation="MLDEGKESARGELQAEASAPRVSVVIVNWNTRELTAQCISTLKE
                     TLEDLPAQIIVVDNASADGSADYIAEHHPDVCLVRSTENLGFARGNNLGFKYARGEYL
                     VLLNSDTIVLKGAVQLLVEYLDAHRDVAAVGGQHLNGEGEFVPTGLRFPTLWSDLSVA
                     AGLNKFGPWVLKKNSQLARLWYVVETQDVDWLSNSFVAVRREVLDQVGPLPDEFFLYG
                     EDIEWYWRMREAGLRVCYVHGAPIVHLENKSSNQLYKNNDKHYRYLDAFYVFASRHRN
                     PLSWRLGWVANALHWSALGARWYLRGRLTKDEDALRRALWLRSFARYHIDQVTGKVAP
                     IAR"
     gene            1470..3764
                     /locus_tag="VGB76_17305"
     CDS             1470..3764
                     /locus_tag="VGB76_17305"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0100707.1"
                     /translation="MTDIQNREIRFALVGCGAIAHSHAQAIAEVAGARCTVLFDLDAA
                     RAETLRQTLCPDASVFTELDKVFAHADAAIVAVPNVHHAALSVQLLRAGLHVLCEKPL
                     ALGVAEASEMIEASEQAGRLLACGFVRRFFDSTALAADALRRGLVGAPVSFEVRESVW
                     NWPLNRATFDPKSAGGGVLIDLGPHVFDQLAAWLGTVEVEGYEDDNGGRVEAFARASV
                     SCRTETGRVPGVVQLSRAYRSVNRARILCTGGYIDIDPHAREEIKIVFGRGEESEGNF
                     QTTAHADPAAAPKGNPFVRQLENFAGAIRGEAALVAPARAALPTVAAIEACYGMRRSA
                     PERWNRQGAGRSAQEGAGNSGVVKRTERKRSYQKILVTGAAGSVGSRLVEMWAEEGRL
                     GELRCMVRSYRTAARIMRYPLEVFEADLTDEAAVRRAAEGCDAIVHLGVGDKASQETE
                     PLLAAARALGIRRFVHMSSAAVYGRALPGRIEAAQEAAPVSKTGEPYADEKAKAEAAV
                     LRECARGLEGIVLRPHMVYGPYLRWSAELMELLARGQVCVIEDGGWCNLIHVDDLVES
                     VRCGLETERGFGQPLFVTDGAPLKWSEYIDAHASLLGLDAPRRMSAEALPVELDWRGW
                     MRASVRPLGAVVKSNEFRSFVFESPAARATLFRAYLALREKKSLRPYLEKLKSGAAGA
                     GGGSRASDNFDETWTLLQLSESRLSPARAETEINFRPRVGFAEGLRRTAEWFAAYGLV
                     PASSSAGTETETEERAEDDLVAAS"
     gene            3826..4479
                     /locus_tag="VGB76_17310"
     CDS             3826..4479
                     /locus_tag="VGB76_17310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0100708.1"
                     /translation="MLKLITLCFKTVRRALVLLFGVPVSFLIDVLERYHLAHWPFVSE
                     ALGIVPFTFGWQFRQEVYRRLGVCRGTGIILNHGTTIGDRRTRIGTDVWMSRDVYVEY
                     AFIEDHVLIGPGAVLLAGRHHHRADDVDVPIKLQGNNPLAPISVGEGAWIGANATVMA
                     DVGAHAIVGAGAVVLKPVAAYAIVGGNPARFIRDRREPASPAVEASRVASAGISEGR"
     gene            4582..5679
                     /locus_tag="VGB76_17315"
     CDS             4582..5679
                     /locus_tag="VGB76_17315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0100709.1"
                     /translation="MRAKNIEATGEGSAGDAAGRQLDVSLVVPVRNEEKSLPALLDSI
                     RRQTHRPAEVIIVDGGSTDETVKTAREIAADSGHNFPELRVIEATEATPGRGRNIGIA
                     AARSEWVALTDAGIRLEPEWLERLVEVVQSDDGMQVVYGNYEASVGSFFERCAALVYL
                     PPKVERAGGRARGPSIASTLLRREVWEAAGGFPDLRAAEDLFFMEQIKRLNFKTGYAP
                     RATVWWQLQPTLGKTFRKFVLYSRHNVWAGRQWDWHYGLARQYALNLIFFALALAHSL
                     WWLALPVAWTLARAAKLIYVRREGRGLLWLSNPVQFAGVVFVMLAIDLATFVGWAQAA
                     WRRGGAASSGSSGATAAQLNVPESSSEHKAR"
     gene            5684..7015
                     /locus_tag="VGB76_17320"
     CDS             5684..7015
                     /locus_tag="VGB76_17320"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0100710.1"
                     /translation="MNEGKTLKTLYLCYFGLREPLVQTQVLPYLRQLSASGEVEVFLL
                     TFEPDKRRAWSASEETQWRAALARDGIRWFSLPYHKSPSALVTPYDIVRGAWEAARLA
                     RRYRIDVLHARGHLPMAMALLARRRANVRLLFDIRGLLAEEYEEAGVWTKNSPQFRAV
                     RAVERAGLRRAAQLVVLTRRLRDRLVEHKLACAERIEVIPCCVDFARFEATHTENGDA
                     RRADESETRANGKNFAAESKAETLAATKAGRFEVVSAGSVTGLYLLEEMGRFFLALKR
                     HRPDAFLRILTMSSAQAATATLEGVGLRAEDFWIGAVKPAEVPAYLRRAGLGLSLRKA
                     TFSQIAASPTKIPEYLAAGLPTVCNAGIGDTDEILTRERVGVIIRNFEAETLDEAARA
                     ALALTDEAEVSARCVEVAHRYFDLASVGGVGYRNAYRRLAAEGARAASETP"
     gene            7012..>7199
                     /locus_tag="VGB76_17325"
     CDS             7012..>7199
                     /locus_tag="VGB76_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100711.1"
                     /translation="MNSAGESLNETISTGTTQATDAPETRNGASESPRALMNLWWERH
                     AATNLVRYDSRLLVLHAV"
BASE COUNT         1261 a         2015 c         2586 g         1337 t
ORIGIN      
        1 agatttgctg atcggggatg cggggaaggc gaagcgggag ctagggtggg agccggagta
       61 tacgtttggg gagatgatca aggagatggt ggaggcggac atgaaggagg cgcagaagga
      121 gtgcggccag agtgtctccg cacgcggcgt gtagagtgga cgagaactga aaagtctgtg
      181 gcggctcagt ttgaaattaa gagatggtct gttggatgct ccggcgtgaa attgaaccgc
      241 cgggtgagag cctgctttgc cggagaactt ttagcttgag ctttggacgg tcgcttccgt
      301 gtggggttga tcgaatttga agaattgtga tgtctaataa agaggttatg cttgacgagg
      361 ggaaggagag cgcgcggggc gagttgcagg cggaggcgtc tgcgccgcgc gtgtcggtcg
      421 tcatcgtcaa ttggaatacg cgcgaactga cggcgcagtg tatctcgacg ctgaaagaga
      481 cgcttgagga tttgcccgca cagattatcg tggtggacaa cgcttcggca gacggcagcg
      541 cggactatat cgccgaacat catccggatg tgtgtctggt gcgaagcacg gagaatctgg
      601 gatttgcgcg ggggaacaat ctcggattca agtatgcgcg gggcgagtat ctggtgttgc
      661 tgaatagcga cacgattgtg ctcaaggggg cggtgcagct tttggtcgaa tatttggacg
      721 cgcaccggga cgtggccgcc gtcggcgggc aacacctgaa cggcgaaggc gaatttgtgc
      781 cgacgggttt gcgtttcccg acgctgtgga gcgacctgtc ggtggccgcc gggttgaata
      841 agtttggacc ctgggtgctg aaaaagaatt cgcagttagc gcgtctctgg tatgtcgtcg
      901 agacgcagga cgtggattgg ttgagcaact ctttcgtcgc ggtgcggcgg gaggtactcg
      961 atcaggtggg gccgctgccg gacgagtttt ttctctacgg cgaagacatc gagtggtact
     1021 ggcgtatgcg cgaggcgggc ttgcgcgtct gttatgtgca cggcgcgccc atcgtccatc
     1081 tggagaacaa gagcagcaat cagctctaca agaacaacga caagcactac cgctatctag
     1141 acgctttcta cgtgttcgct tcgcgccacc gcaacccgct ctcgtggcgt ctggggtggg
     1201 tggcaaacgc tctgcactgg agtgcgctcg gcgcgcgctg gtacttgcgc gggcgtctga
     1261 cgaaagatga agatgcgttg cgtcgtgcgc tctggctgcg ttcgttcgcg cgttatcaca
     1321 tagatcaggt gacggggaag gttgcgccca tcgcgcgctg aacgagagtg tgagcagcgt
     1381 ccgcgcgggc agggcttacg aatttgtgac gggcgagagt gataatttta agatcgtttg
     1441 attgagattc aggatcgttt gattaagaca tgacggatat tcaaaacaga gagattcggt
     1501 tcgcgctcgt cggctgcgga gccatcgcac attcgcacgc gcaggccatc gcagaggttg
     1561 cgggcgcgcg ttgcacagtc ctgtttgatc tcgacgcggc gcgcgccgaa actttgcggc
     1621 agacgctctg cccggatgcg agcgtgttca ccgaactcga taaagttttc gcgcacgcgg
     1681 acgcggcgat tgtcgccgtc ccgaacgtgc atcacgccgc gctctcggtt caacttctgc
     1741 gcgcggggct gcacgtgttg tgcgagaagc cgctcgcgct cggcgtggcg gaagcctccg
     1801 agatgattga agcgagcgag caggcgggac gactgctggc gtgcggtttt gtgcgtcgct
     1861 tcttcgattc gaccgcgctg gcggcagacg ctctgcggcg cgggttggtg ggcgcgcccg
     1921 tcagcttcga agtgcgcgag tcggtctgga actggcctct gaatcgcgcg acgttcgatc
     1981 cgaagtctgc gggcggcggc gtgttgattg atctggggcc gcacgtcttc gatcaactcg
     2041 cagcatggct cggcacggtc gaggtcgagg ggtatgaaga cgacaacggg gggcgtgtag
     2101 aggcgtttgc gcgggcgagc gtcagttgcc ggacggagac ggggcgcgtt cccggcgtgg
     2161 tgcaactcag ccgcgcttat cgcagcgtca atcgcgcgcg catcctctgc acgggcggtt
     2221 acattgatat tgacccgcac gcgcgcgagg agatcaagat cgtcttcggg cgcggggaag
     2281 agtcggaggg gaatttccag acgacggcgc acgcagaccc cgccgctgcg ccgaagggca
     2341 acccgttcgt gcggcagttg gaaaactttg ccggggcgat caggggcgag gcggctctgg
     2401 tcgccccggc gcgggctgct ctgccgacgg tggccgcgat tgaagcctgt tacgggatgc
     2461 gccggagcgc gccggagcgt tggaaccgtc agggcgcggg gcggagcgcg caagagggcg
     2521 cgggcaatag cggcgtcgta aagagaacgg agcggaagcg gagttatcaa aagattctgg
     2581 tgacgggcgc ggcggggtcg gtcggttcgc gtctcgtgga gatgtgggcg gaagagggaa
     2641 ggctcggcga gttgcgctgc atggtgcgga gttatcgcac ggcggcgcgc atcatgcgtt
     2701 atccgctcga agtgtttgaa gcagacttga cggacgaggc ggcggtgcgg cgcgcggcgg
     2761 aaggctgcga cgccatcgtg catctcggcg tgggcgacaa ggcgagtcag gagacggaac
     2821 cgctcttagc cgccgcacgc gcgctcggca tacgtcgctt cgtacacatg agcagcgcgg
     2881 cggtttacgg gcgtgcgttg ccggggcgca tcgaagcggc acaggaggct gcgcccgtgt
     2941 cgaagacggg cgagccgtat gcggacgaaa aggcaaaggc cgaagcggct gtgcttcgcg
     3001 agtgcgcgcg cgggctggag ggcatcgtcc tgcgcccgca catggtctat ggaccatacc
     3061 tgcgttggag cgcggagttg atggagttgc tggcgcgcgg gcaagtgtgc gtgatcgaag
     3121 acggcggctg gtgcaatctg atccacgtgg acgatctggt ggaatcggtg cgctgcgggt
     3181 tggagacgga gcggggcttc ggtcaacctc tgtttgtgac ggacggcgcg ccgctcaaat
     3241 ggagcgagta catagatgcg cacgcgtcgc tgctcggcct cgacgcgccg cgccggatga
     3301 gcgcggaggc tctgccggtc gaactcgatt ggcgcggctg gatgcgcgcg tctgtaaggc
     3361 cgctcggcgc ggtggtcaag agcaacgaat ttcgctcgtt cgtattcgag agtccggcgg
     3421 cgcgtgcgac gctgtttcgc gcttacctcg cgctgcgtga gaaaaaatct ctgcgccctt
     3481 atcttgagaa attaaagtcg ggcgctgcgg gcgcgggcgg cggctcgcgt gcgagcgaca
     3541 atttcgacga gacgtggacg ctgctgcaac tctcagaatc gaggctcagc cccgcgcgcg
     3601 cagaaaccga gatcaacttt cgcccacggg tcgggttcgc ggaaggactc aggcgcacgg
     3661 cggagtggtt cgccgcttac gggctggtcc ccgcgtcttc ctccgccggc accgaaaccg
     3721 agacggagga gcgcgccgaa gacgatctcg tcgccgcgag ttgaattgcc gcgcgccgcc
     3781 tgagacgcca tcagaggctt ggcgcagacg acgacgtgcc acaccatgtt gaagttaatc
     3841 acactctgct tcaagaccgt gcgccgcgct ctggtgctgc tctttggcgt gcccgtcagt
     3901 ttcctgattg atgtcctgga gcgttatcat ctcgcgcact ggccgttcgt ctcggaagcg
     3961 ttgggcatcg tcccgtttac cttcggctgg cagttcaggc aggaagttta tcgtcgtctg
     4021 ggcgtgtgtc gcgggacggg gatcatcctc aatcacggga cgaccatcgg cgaccggcgc
     4081 acgcgcatcg gcacggacgt atggatgagc cgtgacgtgt acgtggaata tgcgttcatt
     4141 gaagaccacg tgctcatcgg acccggcgca gtgctactgg cggggcggca tcaccatcgc
     4201 gcagacgacg tggacgtgcc gatcaagttg cagggcaaca acccgctcgc gccgatcagc
     4261 gtgggagaag gagcctggat cggcgcgaac gcgacggtga tggcagacgt gggcgcgcat
     4321 gcgattgtcg gcgcgggcgc ggtcgtcctg aagccggtgg cggcttacgc gattgtcggc
     4381 ggcaatcccg cgcgcttcat acgcgaccgg cgcgaacccg cgtcgcccgc cgttgaagcg
     4441 tcgcgggtgg cgagcgcggg aatttccgaa ggtcgctaga gtctactcct cagtctctcc
     4501 acgcattctc cccaccgaaa atattcgagg aagtcagcgc ggcgaaattt tagttaagcg
     4561 gagtcgtccg acgtgaagag catgcgagcg aagaacattg aagcgaccgg cgaaggttct
     4621 gccggagacg ctgccgggcg acaactcgac gtgtcgctcg tcgttcccgt gcgcaatgaa
     4681 gagaagtctc tgcccgcgct gctcgattcc atcaggcggc agacgcaccg ccctgcggag
     4741 gtgatcatcg ttgacggcgg ctcgacggat gagacggtta aaacggcgcg ggagatagcg
     4801 gcagactccg gccacaactt cccggagttg cgcgtgatcg aagcgacgga ggcgacgccc
     4861 ggacgcgggc gaaatatcgg catcgcggca gcgcgctccg agtgggtcgc gctcacggac
     4921 gcaggcatcc gccttgagcc ggagtggttg gagcgtctgg tggaagtggt gcagagcgac
     4981 gacgggatgc aggttgttta cggcaactac gaagcctcgg tcggcagttt tttcgaacgc
     5041 tgcgccgcgc tcgtctattt gccgccgaag gtggagcgcg cgggcggacg ggcgcgtggt
     5101 ccatccatcg cctccacgct tttgcggcgc gaggtgtggg aagcagccgg aggctttccc
     5161 gatctgcgcg cggcggaaga cctcttcttt atggagcaga tcaagcgtct gaacttcaag
     5221 acgggctatg cgccgcgcgc caccgtctgg tggcaactgc aaccgacgct cggaaagact
     5281 tttcgcaagt tcgtgctcta ctcgcgtcac aacgtctggg cggggcgaca atgggactgg
     5341 cattacgggc tggcgcggca atacgcgctc aacctgatct tcttcgcgct cgcgctcgca
     5401 cacagcctgt ggtggctggc cttgcccgtc gcgtggacgc tcgcgcgcgc cgccaagttg
     5461 atctacgtgc ggcgcgaagg gcgcgggctg ctgtggctct cgaaccctgt gcagtttgcg
     5521 ggcgtcgtct tcgtcatgct ggcgattgat ctcgccacgt tcgtcggctg ggcgcaggcc
     5581 gcgtggcgac gcggcggcgc ggccagcagt ggcagcagcg gcgcgacggc ggcgcagttg
     5641 aacgtgccgg agtcgagttc ggagcacaag gcgcgataac gagatgaacg aggggaagac
     5701 tttgaagacg ctctacctgt gctacttcgg tctgcgcgag ccgctcgtgc agacgcaggt
     5761 gctgccctat ctcagacaac tcagcgcgag cggagaggtg gaagtcttcc tgctcacttt
     5821 tgaaccggac aagcggcgcg cgtggtctgc gtcggaagag acgcagtggc gcgctgcgct
     5881 tgcgcgcgac gggatccgct ggttctctct gccgtatcac aaaagccctt ccgcgctcgt
     5941 tacgccttac gacatcgtgc gcggggcatg ggaggcggcg cggctcgcgc gtcgttatcg
     6001 aatagacgtg ctgcacgcgc gcgggcatct gccgatggcg atggccttgc tggcgaggcg
     6061 tcgggcaaac gtgcggctgc tgttcgacat tcgcgggctg ctcgcggagg agtacgaaga
     6121 ggcgggcgta tggaccaaaa actcgccgca gtttcgcgcg gtgcgcgccg tcgagcgcgc
     6181 ggggctgcga cgggcggcgc aactcgtcgt cttaacgcgg cgtctgcgcg accgtctcgt
     6241 cgagcacaaa ctcgcgtgcg ccgaacggat cgaagtcatc ccctgctgcg ttgacttcgc
     6301 gcgcttcgag gcgacgcaca cggagaacgg cgacgcgcgg cgcgcggatg agagcgagac
     6361 gcgcgcgaac gggaagaact tcgcggcgga gtcgaaggcg gagacgttgg cagcgacgaa
     6421 ggcggggcgt ttcgaagtcg tctcggcggg gtctgtcacg gggttgtatc tgctcgaaga
     6481 gatggggcgt ttctttctcg ccctgaaacg gcacaggccg gacgctttcc ttcggatact
     6541 cacgatgtcg tcggctcagg ccgcgacggc gacgctcgaa ggcgtgggct tgcgcgcgga
     6601 agatttctgg atcggcgcgg tcaagcctgc ggaagtgccc gcctatttgc gccgcgcggg
     6661 actcggcctt tcgcttcgca aggccacctt ctcgcagatc gccgcctcgc ccacgaagat
     6721 tcccgaatac ctcgccgccg gtctgcccac cgtctgcaac gcaggcatcg gcgacacgga
     6781 cgagattctg acgcgcgaac gcgtcggcgt catcatacgc aacttcgaag ctgagacgct
     6841 cgacgaagcg gcgcgcgccg ccctcgctct cacggacgaa gcggaggtga gcgcgcgttg
     6901 cgtcgaagtg gcgcatcgtt attttgatct cgcgagcgtc ggcggcgtcg gctatcgcaa
     6961 cgcgtatcgc cgtctggctg cggagggcgc gcgcgccgcg tccgagactc catgaattct
     7021 gcgggtgaaa gtttgaacga aacgatctca accgggacga cacaagcgac ggacgcgccg
     7081 gagactcgaa acggcgcgtc cgagtctcca cgcgcgctga tgaatctctg gtgggagcgg
     7141 cacgcggcga cgaatctggt gcgttacgat tcgcgcctgc tcgtcttgca cgcggtcga
//
LOCUS       DASWNS010000503         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_408057, whole genome shotgun sequence.
ACCESSION   DASWNS010000503 DASWNS010000000
VERSION     DASWNS010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408057"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VGB76_17330"
     CDS             <1..462
                     /locus_tag="VGB76_17330"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100712.1"
                     /translation="MSYPGTVYFEGTNLTEGRGTDRPFEQIGAPWLDAREVARVMNER
                     LLPGVRFEAITMSVVPTAAKHKGQTIPAIRFHVTDRRTYRPVRTSLLLIDEIRKRHPR
                     DFAWTATIDRLTGSDKVRLAIEGGRLHPLLEEWDRQAAEFRESRKPFLLYP"
     gene            complement(803..1810)
                     /locus_tag="VGB76_17335"
     CDS             complement(803..1810)
                     /locus_tag="VGB76_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0100713.1"
                     /translation="MSDAALQLSAPDERQDSVALRNAIRVWADGTTNKLAHNPEALLR
                     DKRTAVESFFRMAGKSPEGVDVTDIETWRRRMEEQGLKPNTVYTRVSLLASFFQWLMR
                     DPVLGQHLRQNPVRPAMPKRPQSYQTEATKAYTDEEMNALLATVRTEADGGSVKAMRD
                     YALLLIYFLSGLRRREVISLRGRDVELKGEGMIIHYRRKGGRYQGRELLESSAIGALL
                     SYLEASGRGDVLGSERPVWTRHDRAGNAGAPLCSHAFAKNLKAYAKSAGVKNARIHRT
                     RHTFGRLVAEQTGSLVETQDALDHANLSTTRVYVERIVVKKDKFSKGIAGRLQGKGVP
                     S"
     gene            complement(1807..2244)
                     /locus_tag="VGB76_17340"
     CDS             complement(1807..2244)
                     /locus_tag="VGB76_17340"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0100714.1"
                     /translation="MSYLVDTNILLRSAQSTHPMHADATRAVGELLARSEILSIIPQN
                     IIEYWSVATRPNEANGLGMSVDETAAEVAGLQSIFQLLPDNAEIFSEWKRLVSKHEVK
                     GKEVHDARIVAAMLAHRVTHLLTFNTADFKRYSQITAVNPRDV"
     gene            complement(2241..2516)
                     /locus_tag="VGB76_17345"
     CDS             complement(2241..2516)
                     /locus_tag="VGB76_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100715.1"
                     /translation="MTLTIDLPPALEERLQQESAREGLAAEEYARKVLEERLLPVAAQ
                     GDAGGNEERLRSYREWVGGHAGHSAPPLSDEAISRESIYREREDRQL"
     gene            2789..3481
                     /locus_tag="VGB76_17350"
     CDS             2789..3481
                     /locus_tag="VGB76_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100716.1"
                     /translation="MPDIIPASEKQLSRFLFAVRHVERRPATDTKRGRPSRWPREKLL
                     EAAALLRSILERETSGRVSLNSFLGQYLPILKFPSDVTDALSKGSINLHEAAQLSRLA
                     PERLDCTSATAKTSREEVLRAHVLAHGSQNSLRARVKEMLGEVVTVSSEQMTSVVQKV
                     DELLEINPSDKRHFFYEEMKRLFYAMREIRPEDIDDESLDQFMAAADQLSNAIHAIEL
                     RSRKKKTSKLSI"
     gene            complement(3968..>4354)
                     /locus_tag="VGB76_17355"
     CDS             complement(3968..>4354)
                     /locus_tag="VGB76_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100717.1"
                     /translation="AAPLGELFRELEREVIGKNSASLQQWQDLRELLVTELKIAAART
                     TVSNVPAFLTEHLRRRLWRVDKKRATEIAAAEQGNQAQAINEKEKRKCPDCAGTGFWY
                     PEGLDKGVARCTHLKLSSVPPTGETD"
BASE COUNT          910 a         1228 c         1246 g          970 t
ORIGIN      
        1 atgagctatc ccgggacggt ctactttgag ggcactaacc tgaccgaggg taggggcacc
       61 gaccggcctt tcgagcagat cggcgcccct tggctggacg cgcgtgaggt cgcccgtgta
      121 atgaacgagc ggctgctgcc gggcgtccgc tttgaagcga ttacgatgtc ggtggtgcca
      181 acggccgcca agcacaaggg gcagactatc cctgcgatcc gcttccacgt caccgatcgc
      241 cggacgtaca ggccggtgcg cacgtcgctc ctgctgatcg atgagatacg caaacggcat
      301 ccgcgcgatt tcgcttggac ggcgacgatt gatcgcctca cgggatcgga caaggtcagg
      361 ctggccatcg aaggcggtcg gctccatccc ctcttggagg agtgggacag acaggccgcg
      421 gagttccgtg agagtcgcaa acctttcctg ctctaccctt gaactattcg acacagctgt
      481 cgcgcgcgag catatagtct caagtagaga ggctctggcg aaggccatca agctagtaac
      541 gaaagccgac atccggggct ggtgcaaaca ttgcggttac tcactctcac gcaagtgaga
      601 accggtatag gtatgtcttg ggaggaaatt tcaacgtacg gtggcatacg gccaccgcat
      661 ttccgcgcga ccacaagagt tttgtgcgat gagatcacaa agtccgaaga cgtgttcaga
      721 acattggaga ggatgaagaa agccgaatct aagacagact ggtgattgca catgttgccg
      781 tcgtggaatg cggcaagagg gctcaggaag gaacaccctt accctggagc cgtccggcta
      841 tccctttgct gaatttatcc ttcttcacga cgatccgctc gacgtagact cgtgtcgtcg
      901 agaggtttgc gtggtcgagc gcgtcctgcg tctcaacgag gcttcccgtc tgttcggcca
      961 ccagcctgcc aaacgtatgt ctggtgcggt ggatgcgagc gttcttcacg ccggctgact
     1021 tcgcgtaggc cttcagattt ttggcgaagg catgggaaca gagcggcgca ccggcattgc
     1081 ccgcccggtc gtgcctggtc cataccggcc gttccgagcc gaggacgtcg cccctacctg
     1141 aagcctccag atatgagagc aaagcaccga tggccgagga ctccagtaac tccctgccct
     1201 gatagcgccc gcccttgcgc cggtaatgga tgatcatccc ctcgcccttg agctccacgt
     1261 ccctgccgcg tagcgaaatc acttcgcgcc gtcgcagccc actcaggaag tagataagca
     1321 ggagcgcgta gtcgcgcatg gccttcaccg acccgccatc agcctccgtc ctgacggtgg
     1381 cgagcaaggc gttcatctcc tcgtccgtgt aagccttcgt cgcctcggtc tggtaagact
     1441 gaggtcgctt cggcatcgcg ggtcgcaccg ggttctggcg gaggtgttgg ccgaggacgg
     1501 ggtcacgcat caaccactgg aagaaggaag cgaggagcga gacgcgcgtg tagacggtgt
     1561 tcggcttgag tccctgctcc tccatgcggc ggcgccacgt ctcgatgtcc gtgacatcca
     1621 ccccctccgg cgacttcccg gccatccgga agaacgattc gacggcagta cgcttgtcac
     1681 ggagcagggc ttccgggtta tgcgccagtt tattggtcgt gccgtcggcc cacacgcgaa
     1741 tcgcgttgcg cagcgcgacc gagtcctggc gttcgtcggg ggcagatagt tggagtgccg
     1801 cgtcgctcat acgtctctgg gattgacggc ggtgatctgc gagtatcgct tgaagtcggc
     1861 cgtattgaag gtcagcaggt gcgtaacgcg gtgagccaac atggccgcga cgatccgcgc
     1921 atcgtgcacc tctttgccct tcacttcgtg tttgctaacc aaccgcttcc actccgagaa
     1981 gatctctgcg ttgtccggaa ggagctggaa gatactttgc agtccggcga cctctgccgc
     2041 ggtttcgtcc acgctcattc ccagaccgtt agcctcgttg gggcgggtgg ccacggacca
     2101 gtactcaatg atgttctgcg ggatgataga aagtatctcg cttcgggcta gaagttcgcc
     2161 gactgcgcgg gtggcgtccg cgtgcatagg gtgtgtggac tgagccgagc gcaggaggat
     2221 gttggtgtcg acgaggtagc tcatagctgc cggtcttcgc gctcccggta aatgctctcc
     2281 cggctgatcg cctcgtcgga gaggggcggc gcggagtgcc cggcgtggcc gccaacccat
     2341 tcacggtaag accgaagcct ttcctcattc cccccggcgt ccccctgcgc ggcaaccggc
     2401 agaagccgct cttcgaggac tttgcgggcg tactcttcag cggcgaggcc ctcgcgggcc
     2461 gactcctgct ggagccgctc ttcaagcgcc ggcggcagat cgatggttaa tgtcacttcc
     2521 ctgctccttt ccgaccggag tatagcatca ttttgattct ccgttaaggt tacactaatc
     2581 gtagtttagt gtaaccttgt attcacaaat taactattat tgctctttag attggtacaa
     2641 tcagtgtatg atgttcaagc tgtgaaacca ttccgaatta ggaggccttg agagacgaat
     2701 gcgtgcctca cgtcggcgac gacgtcagtt agatacgctc gcatcgcccg aagccgttcg
     2761 cctgatctgt gggcaactac gcgagcgaat gcccgacatt atcccagctt ctgaaaagca
     2821 gttatcgcgc tttctatttg cggtgcggca tgtcgagcgg cgccccgcga ccgacacgaa
     2881 gagaggcagg ccctcacgat ggccgcgcga aaagctcctc gaagctgcag ctttgctcag
     2941 atccatactc gaacgtgaaa catcgggtag agtttctctc aatagctttc tcggccagta
     3001 tcttcccatc ctcaaattcc cttccgacgt gacggacgca ctctctaaag gctcgataaa
     3061 tctccatgag gccgcgcaac tctcccggct ggcgcccgaa cgcttggact gtacatcggc
     3121 cacggcaaaa acctcccgcg aggaggtgct ccgggcgcat gtcctcgctc acggatctca
     3181 gaacagtctg cgggcacgcg ttaaagagat gctaggcgaa gtcgtgacag tctcgtccga
     3241 gcagatgacg agcgttgtac agaaagtcga cgaattgctt gagatcaacc cctccgacaa
     3301 gcgccacttc ttttacgaag agatgaagag gctattttac gccatgaggg aaatccgtcc
     3361 ggaagacatc gacgacgaat ccttagacca attcatggcg gcggctgatc aactctcgaa
     3421 cgccatccac gccatcgaac tacgaagcag gaagaagaag acttctaaac tcagtatctg
     3481 atagagaagt atcttgatat ttcaccctga aaaataaatt acagggtgaa gaaaatattt
     3541 cagggtgaaa ttttcccaac agcagaaaaa tcaggctaaa caaaggtgtt gggtattgcg
     3601 aggttttatg aggcgggtaa gctaatgcgg aaatggatgc cgcggcggtc tgccgggtcg
     3661 taatccttga gccggatcac aaagccccgg ctcgcgaatt cctttatcgg aattagcctg
     3721 tgccaatgca aacaacgatt cttaattggt gtaaaaagat taattcagtg aacgtgaaac
     3781 cttgtgtgta acaaattttc ggggtgcatt ttcatgaagt tatgggaata taaattcaaa
     3841 agccaagttg cgctctgaga tataccattg agcttaaata tcttggccgg tgtgttactt
     3901 gcttatactt gcgctttttt atctgtaatt agcttaatgt gtaaaggggt gaaattatgt
     3961 gaccggatca gtcagtttct ccggttggcg ggacggatga cagtttgagg tgcgtacacc
     4021 gggcgacacc tttgtccagc ccctccggat accagaagcc tgttcccgcg cagtccgggc
     4081 acttccgctt ctctttttca ttgattgcct gcgcctgatt tccctgctcg gcagcagcaa
     4141 tctcagtggc gcgtttctta tccactctcc atagtctgcg ccgtaggtgc tcggtgagaa
     4201 acgccgggac gttcgagact gtggtacgcg cggcggcgat cttcaattcc gttactaata
     4261 gttcgcgtaa gtcttgccat tgctggagag atgcggagtt tttgccgatc acttccctct
     4321 ctagttcgcg gaaaagctcc cccagcggcg cagc
//
LOCUS       DASWNS010000504        10579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_149255, whole genome shotgun sequence.
ACCESSION   DASWNS010000504 DASWNS010000000
VERSION     DASWNS010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149255"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..628)
                     /locus_tag="VGB76_17360"
     CDS             complement(128..628)
                     /locus_tag="VGB76_17360"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY0100718.1"
                     /translation="MSYISPKAAVRESRIHGRGLFAVEAFARDEIVAVKGGHIFDRRT
                     LREVEGVLGAAEIQIGEDLFIGALTQNEREGCMIFSNHSCEPNIGVRGQIVFVAMRDI
                     EAGEELTHDWATTDDDTYRLECRCGAQSCRKIITGQDWRREDLQAKYGRYISLYLLEK
                     INGATL"
     gene            complement(659..877)
                     /locus_tag="VGB76_17365"
     CDS             complement(659..877)
                     /locus_tag="VGB76_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100719.1"
                     /translation="MASLLNERFVCLASDCDRPEPEIRAIGAQHMAYARALPFCLYLN
                     SDGAFVHGTQGGLSLYSFREDLERVSKS"
     gene            complement(923..1063)
                     /locus_tag="VGB76_17370"
     CDS             complement(923..1063)
                     /locus_tag="VGB76_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100720.1"
                     /translation="MSDTRKSSHPHFDDRGAVRWHTRFADALEEARREGKFIFVEYGR
                     YA"
     gene            complement(1056..1616)
                     /locus_tag="VGB76_17375"
     CDS             complement(1056..1616)
                     /locus_tag="VGB76_17375"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEY0100721.1"
                     /translation="MGTEIEKKYRIGSEEGGRLRSRLRAVGAEARGEEFEENTLYAGP
                     GLESGNRVLRLRRVGERAIFTFKESMANSSGIKRRREDETAVADADALADILDALGYK
                     PAVVYEKRRETWLVAGVEVVLDELPFGLYVEIEGEEEPILEAERLLELSDAAEEPRSY
                     PQLTLLHGKSRDTVVEARFTGGTGDV"
     gene            complement(1733..3088)
                     /locus_tag="VGB76_17380"
     CDS             complement(1733..3088)
                     /locus_tag="VGB76_17380"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0100722.1"
                     /translation="MKKLWIIIKREYWQRIRTKGFIITSLLGPLVMVGFTVGSGLVIT
                     MKTGGATKVAVVDLTGRMYEGVRESITTRRGNEGEGEQKKQASPVEAAPDSEAQLRQA
                     AKALGGHYEVEQVTPGGRPLEEIKNELNARLRKNELGVYIVLPEKILEEGRVEYYGRN
                     VSDLITTAQLRHSLNRAVVEQRLREANIDQNRVRQLSKEIKMEMTKVSEAGVERDSGG
                     GFGLALFMGLFIYMAILMYGQAILASVVEEKTTRVSEVLFSSVRPFHLMAGKLAGVSL
                     VAITQYAIWAAVFGLFVLYGVGMLAASGMEVPLPRISPSLVIYTLLFFIVGFLMYATI
                     YVLVAAMVTTEKEAGQMALPVIFLSILSIYMAFPVIRNPNSSFAFWVSIAPFFSPITM
                     LVRIVTETPPFWQIALSLAVGLATVVLFVWLAARIYRVGMLMYGKSASIPEVVRWLRQ
                     S"
     gene            complement(3085..3999)
                     /locus_tag="VGB76_17385"
     CDS             complement(3085..3999)
                     /locus_tag="VGB76_17385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0100723.1"
                     /translation="MSQDVVTLALERVSKKFDDFVAVDELSLQVRAGRIFGLIGPNGA
                     GKTTTIRMIVNILVPDSGRIELFGEQMSERLQDRIGYLPEERGLYKKMRVGEQLRYFG
                     ELKGLTAREAETQLDRWLARLKLSEWKNHKTDELSKGMQQKIQFITTVMHAPDLLILD
                     EVFSGLDPVNAELLKDVLLELKSERRTIIFSTHMMSQAEELCDDICLINRSRKVLQGS
                     LREVKSSFGRRAVALRAEGGDGTLTDPQLVAGVKPYGDYVEVLLAEGADAQELLHRLV
                     DGGARITRFEQVEPSLHDIFLQQVGESA"
     gene            complement(4292..4681)
                     /locus_tag="VGB76_17390"
     CDS             complement(4292..4681)
                     /locus_tag="VGB76_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100724.1"
                     /translation="MSMIPGRAMRALLKAGVAPRHITAVGASKGSNIAMHTSSLLRNR
                     DVNFVLMAGCGAQTLKKQSLNLYGRVLSIYDASDEGVGTGETLFARSEGLSSRNEIKI
                     NLGLGHGFLYRPHKEWIEPAIEWAKQS"
     gene            complement(4697..6295)
                     /gene="serA"
                     /locus_tag="VGB76_17395"
     CDS             complement(4697..6295)
                     /gene="serA"
                     /locus_tag="VGB76_17395"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY0100725.1"
                     /translation="MDNIKILVSDDVSESGLEPLRAAGFAVEKKTGLKTDELREIVRD
                     YDGLVVRSETKVTAALMDEAVRLRAIGRAGVGVDNIDVAAATTRGIIVMNAPDGNTMT
                     TAEHTLALLLALARRVPQAHASLQGGKWERKKFVGVELRGKTLGVVGLGRIGRVVASR
                     ARGFEMKVLAFDPFVAPEQARDMELELAPLEEVCARADFLTVHTPLTAETRGIIGARE
                     FARMKPEVRIINCARGGLIDERALYEALKAGRVAGAALDVFEEEPPSQSNPLLQFAEV
                     ITTPHLGASTREAQEGVAVTVAEQMRDYFQTGALRGAVNAPALGAQELHTLSPYLVLA
                     ERLGRFQAQLLDEAVREVRIEYAGELAELEAAPVTRAFLAGLLRDMSARVNAINASLI
                     AEERGITVTNSYTRGAGNDPAHAVTTRVLTTGGEQTVSGALFGSNGDGRITEINGFRI
                     EAIPSGHMVVTRNHDVPGVIGRVGTILGAHGVNIGRFHLGRRERGGEAMAVIETDAPL
                     DDATLDELQAFEQIVSARRIALES"
     gene            complement(6415..6825)
                     /locus_tag="VGB76_17400"
     CDS             complement(6415..6825)
                     /locus_tag="VGB76_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0100726.1"
                     /translation="MANDSFFSTWTPRILSVLRIVAALLMMQHGAQKLFGFPAGAQGS
                     QTPPLLSMMGFVGILEFFGGLLLLLGLFTRPVAFLLSGLMAVAYFMAHAPQGLWPVLN
                     RGELAALYSFVFLYLAVAGGGVWSVDYCWRRRES"
     gene            complement(6957..7211)
                     /locus_tag="VGB76_17405"
     CDS             complement(6957..7211)
                     /locus_tag="VGB76_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100727.1"
                     /translation="MEDAERTKPGAGNGDDKSAQTAARQDEATSSDTLNDLQQTQTDS
                     AASQGETSGASDSSSAPAPDGTPDTTRGGSADGRDSGGPM"
     gene            complement(7224..7838)
                     /locus_tag="VGB76_17410"
     CDS             complement(7224..7838)
                     /locus_tag="VGB76_17410"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY0100728.1"
                     /translation="MKVVKESSDELNPSVVVTTAFIGRARRERTLRDYVALGVATCGV
                     GLIPLAPGTWGSLVGVGLYLAARAASVQAFLYAEARGWSTPPLESLRTTVVLLMLIVM
                     TIAGIWAATRCESLLARKDPGAVVIDEVIGQILTFVFLPVGIGAWAIVVGFLAFRLFD
                     IWKPYPIRRLESLEAGLGIMADDVLAGVYAATLMSLLSSLYLLF"
     gene            complement(8021..9376)
                     /gene="rimO"
                     /locus_tag="VGB76_17415"
     CDS             complement(8021..9376)
                     /gene="rimO"
                     /locus_tag="VGB76_17415"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY0100729.1"
                     /translation="MKKIGFISLGCPKNLVDSEVMMGQLAAAGYEITTDEAEAETLVV
                     NTCGFIDSAKKESVDAILEAARMKTAGKAKRLVVAGCLVERYRDELRAEIPEVDAFIG
                     TNQINDITKVCDDKVNTRSLPVITLGNQSATYLYDESTPRVLATPGYSAFIKIAEGCD
                     RPCAFCFIPQMRGHFRSRRFGSVVSEAQALAARGVKELILVAQDSSRYGEDLEQPDGL
                     AHLLRELSRLDGIEWVRVMYTYPTHISDLFLDVMASEPKAVKYLDMPLQHASQNVLKL
                     MRRGGNRASLERLIERVRRRVPGIGVRTTFITGFPGEREEDFNELLAFVRNVEFDRVG
                     VFTYSDEEGTPAYDLPDKVDPKVAAKRRDRLMKEQRRISLKRNRARVGSTARVLFEGA
                     AKETDLLWQGRLESQAPDIDGVVLINDAPEGFAPAPGDFVNVLITDAHEYDLVGSIIE
                     P"
     gene            complement(9525..>10579)
                     /locus_tag="VGB76_17420"
     CDS             complement(9525..>10579)
                     /locus_tag="VGB76_17420"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEY0100730.1"
                     /translation="LAPYPRTFDRYQKVPDDLDPRIRQLTRGLIVGTGSDNRYDAARV
                     IEAYFQQNFRYTLEQKSGGASDPLADFMFRVREGHCEYFSTAMAVMLRSEGIAARVVN
                     GFQLGDYNDAADVYTVRQSDAHSWVEVYFPETNAWVTFDPTPVAGRPGNNDETGFWRG
                     GLGKYAEALELFWIQYVVAYDKQEQRSLATSVRGQFGVYRQTVGQTFAELRAELSGWW
                     RQVTNGGDAQGLIRATNLPVVVALVLLAGLLLMVVARVRRWDFRRGLNFWRREGKNYS
                     SVVFYERMTKALESRGLLRAPDETPLEFAAATGMPEATLLTRSYNRVRFGAHELTTAE
                     AARIEEWLRRIEKERE"
BASE COUNT         2065 a         3371 c         3029 g         2114 t
ORIGIN      
        1 gcagatactc tgcgctggaa cgtgcgcccg tccaatattg acgatcatcc ggagcgcgtg
       61 tgggattgga gctatccgca gtttatagcg gtggagcctg ccgagccgga gaaacgttaa
      121 agacgcttca cagtgtcgct ccgttgatct tctccagcag gtacaaggat atatatcgcc
      181 cgtacttcgc ctgtaaatct tctcgtcgcc aatcctgccc cgtgatgatc ttccggcacg
      241 actgcgcgcc gcagcggcac tccagacggt aagtatcgtc gtcggtcgtc gcccaatcgt
      301 gcgtcaactc ttctccggcc tcgatgtcgc gcatggcgac gaacacgatc tgcccgcgca
      361 cgccgatgtt cggctcgcag gaatgattgc tgaagatcat gcacccctct cgttcgttct
      421 gtgtgagcgc gccgatgaag aggtcttcgc cgatctgtat ttccgcagca cccagcacgc
      481 cttcgacttc gcgcaaagtg cgccggtcga agatgtgccc gcctttaacc gcgacgatct
      541 catctcgcgc gaacgcttca accgcgaaga ggccgcgccc gtgaatccgg ctctccctca
      601 ccgcagcctt cggggatatg taagacaaat tttgcatagg attgacggtg ggcttcgttc
      661 aacttttgct cacgcgctcc aaatcttcgc gaaacgaata gagcgagagg ccgccctgtg
      721 tgccgtggac aaacgctccg tcgctgttga ggtagagaca gaacggcagc gcgcgtgcat
      781 aggccatgtg ctgcgcgccg atagctctga tctccggttc gggccggtcg caatcggaag
      841 cgagacagac aaaacgctcg ttgagcagag aggccacgga cgcttgcggg atgatgccct
      901 cgacgagagc acggcagttg cctcaagcgt atcgcccgta ctcgacgaag atgaacttcc
      961 cttcgcgcct cgcctcttcg agcgcgtcgg cgaagcgcgt gtgccagcgc accgcgccgc
     1021 gatcatcaaa gtgcgggtgc gaacttttgc gcgtgtcaga catcgcccgt tcctcctgtg
     1081 aatcgtgctt cgaccactgt gtcgcggctc tttccgtgta agagcgtcaa ctgcgggtac
     1141 gacctcggct cctccgccgc gtcggagagt tcgagcaggc gttcggcttc gagaatcggc
     1201 tcttcctcgc cttcaatctc gacgtagagg ccgaacggca gttcgtctaa caccacttcg
     1261 acgcccgcca cgagccacgt ctccctgcgc ttctcataca cgacggctgg cttgtagccg
     1321 agcgcatcga gtatgtctgc gagcgcgtcc gcgtctgcta ccgccgtctc atcttcgcgg
     1381 cgtcgtttga tgccggacga attcgccatg ctctctttga aagtgaagat cgccctctcc
     1441 ccgacgcgtc gcaggcgcaa cactcgattg ccgctctcaa gccccggccc cgcgtacagc
     1501 gtgttctctt caaactcctc gccgcgcgcc tctgccccga ccgcacgcag acgacttcgt
     1561 agccgcccgc cctcctcgct cccaatccgg tacttcttct cgatctctgt gcccataaaa
     1621 aaatgcccgt ggtcagtcgt tcgtctcaat acgtcgcatt gcgccgctaa ctttaccaac
     1681 taactcgcgc agcacaaaca acggacaact gatcacggac aacagaccgc ctctaactct
     1741 gcctgagcca gcgcaccact tctgggatgc tcgcgctttt gccgtacatc aacataccga
     1801 cgcgatagat gcgcgcggcg agccagacga agagcacgac cgtcgccagc ccgacggcga
     1861 gcgagagcgc gatctgccag aagggcggcg tctccgtgac gatgcgcacg agcatcgtga
     1921 tgggcgagaa gaacggcgcg atggacaccc agaaggcgaa cgaagagttg ggattccgta
     1981 tgaccgggaa ggccatgtag atgctgagga tggagaggaa aatcaccggc agagccatct
     2041 gtccggcctc tttctccgtc gtcaccatcg cggcgaccag cacgtagatc gtcgcgtaca
     2101 tcaaaaaccc gacgatgaaa aagagcagcg tgtagatcac taaagacggc gagatgcgcg
     2161 gcaaaggcac ctccatcccg ctcgccgcca gcatcccgac gccgtagagc acgaacaggc
     2221 cgaagaccgc cgcccagatc gcatactgcg tgatcgcgac gagcgagacg cccgccagtt
     2281 tccccgccat caaatgaaac gggcgcaccg acgaaaagag cacttccgaa acgcgcgtcg
     2341 tcttctcttc gaccaccgac gcgagaatcg cctgcccgta catcagaatc gccatgtaga
     2401 tgaaaagacc catgaacagc gccagcccga aaccgccgcc gctgtcgcgc tcaactccgg
     2461 cctcgctcac cttcgtcatc tccatcttga tctctttcga caactggcgc acgcggttct
     2521 gatctatgtt cgcctcgcgc aaccgttgtt cgacgacggc gcgattcaaa ctgtgacgaa
     2581 gttgcgccgt cgtgatcagg tcgctcacgt tgcgcccgta gtattccaca cgcccctcct
     2641 ccaaaatctt ttccggcagg acgatgtaaa ctcccaactc gttcttccgc aagcgcgcat
     2701 tcaactcgtt cttgatctct tcgagcggac gaccgcccgg cgtcacctgc tcaacttcgt
     2761 aatgcccgcc gagcgccttt gccgcctggc gtagctgcgc ctcactatcc ggcgcagcct
     2821 ccacgggtga cgcctgtttc ttttgctccc cttcgccttc attgccgcgc ctcgtcgtga
     2881 tcgactcacg cacgccctca tacattcttc ccgtgagatc gacgacggcg accttcgttg
     2941 cgccccccgt cttcatcgtg atgaccagac cggagccgac cgtgaaaccg accatgacga
     3001 gggggccgag cagggacgtg atgatgaaac ccttcgtccg aatgcgctgc cagtattcgc
     3061 gcttgatgat gatccatagt ttcttcatgc gctctctccc acctgctgca aaaagatgtc
     3121 gtgcaggctc ggttcgacct gttcgaagcg cgtgatgcgc gccccgccgt ccacgagacg
     3181 atgcaacaac tcctgcgcgt ccgccccttc ggccagcaac acttcgacgt aatcgccgta
     3241 aggtttgacg ccagccacga gttgcggatc ggttaacgtt ccgtcgccgc cctccgcgcg
     3301 caaggccacc gcgcgccgac cgaaactgct cttcacttcg cgcagacttc cctgcaagac
     3361 tttgcgcgaa cgattgatga ggcagatgtc gtcgcagagt tcttcggcct gcgacatcat
     3421 gtgtgtggag aagatgatcg tgcgccgctc cgacttcagt tcgagcagca catctttcaa
     3481 gagttcggcg ttgacgggat cgaggccgga gaaaacttcg tcgaggatga gcaggtcggg
     3541 cgcgtgcatc acagtggtga taaattgaat cttctgctgc atgccctttg agagttcgtc
     3601 ggtcttatgg ttcttccatt cgctgagttt caggcgcgcc agccagcggt cgagttgcgt
     3661 ctcggcctcg cgcgccgtga gtcccttcaa ctctccgaag tagcgcaact gctcgcccac
     3721 gcgcatcttt ttataaaggc cgcgctcttc cggcagatag ccgatgcggt cctgcaaacg
     3781 ctcggacatc tgctcgccga agagttcgat gcgcccggaa tcgggcacaa ggatgttgac
     3841 gatcatgcgg atggtggtgg ttttgcccgc gccgtttggt ccgatcaggc cgaaaattct
     3901 tccggcgcga acttgcaggc tgagttcgtc aacggccacg aagtcgtcga attttttcga
     3961 gacgcgttcg agcgcgaggg tcacaacgtc ttgactcaaa agttttcttc acctccggcg
     4021 gacgcctcga agctaccggc gtgtgggcgt cgggacaaat tcatgtgcaa gatttatacg
     4081 ggcggcaggt gttgaaagtt tttcgcgcgc ggcaaccagc aaggtgagaa aagcgcgcgc
     4141 gtgcggggct tagagcagct tgagattgag agcaacaaaa accgggactc gacgcaagcg
     4201 gcgcggagat gtgcgcagaa gaggcgcgtg gagagttgat cgatcaactc tctacgcgcc
     4261 tccgcgtcga aggtttaaag gccagggatt gttaagattg tttcgcccat tcgatggcgg
     4321 gttcgatcca ttctttgtgc gggcgataga gaaagccgtg tccaagtccg aggttgatct
     4381 tgatttcatt gcggctgctc agtccttcgg aacgcgcgaa gagagtttca cccgtcccga
     4441 cgccttcgtc gctcgcatcg tagatcgaca gcacgcggcc atacaggttc aacgactgtt
     4501 tcttcaacgt ctgcgcgccg caaccggcca tgagcacgaa attaacgtcc ctgttcctga
     4561 gcagcgacga agtgtgcatc gcaatgttcg accctttcga cgcgccgacg gctgtgatgt
     4621 ggcgcggcgc gaccccggcc ttgagcagcg cgcgcattgc ccgtcccgga atcatgctca
     4681 tcaggctgcc ctctcttcaa gactcaagcg cgatgcgtcg cgcagagacg atctgttcga
     4741 acgcttggag ttcatcgagt gtggcatcgt cgagcggcgc gtcggtttcg atcacggcca
     4801 tcgcttcgcc gccacgctcc cggcgtccca gatgaaagcg accgatgttg acgccgtgcg
     4861 cgccgaggat cgtgccgacg cgaccgatga cgccgggcac gtcgtggttg cgcgtgacga
     4921 ccatgtgccc cgacgggatg gcttcgatgc gaaagccgtt gatctccgtg atgcgcccgt
     4981 cgccgttgga accgaagagt gcgccggaga cggtctgctc gccgcccgtc gttaagacgc
     5041 gcgtggtgac ggcgtgcgcc gggtcgttcc ccgcgccgcg cgtgtacgag ttggtcacag
     5101 tgatgccacg ttcttccgcg atgagagacg cgttgatggc gttgacgcgc gcgctcatgt
     5161 cgcgcagcag ccctgcgagg aaagcgcgcg tcacgggcgc ggcctccagt tcggccaact
     5221 cgcccgcata ctcgatgcgc acttcgcgca cggcctcatc caacaactgc gcctgaaatc
     5281 ttcccagacg ttcggcgagg acgaggtaag gcgagagcgt gtgcaactcc tgcgcgccga
     5341 gagccggagc gttgaccgcg ccgcgcagcg cgcccgtctg aaagtagtcg cgcatctgct
     5401 cggccaccgt gacggccaca ccctcctgcg cctcgcgcgt cgaagcgccg agatgcggcg
     5461 tcgtgatcac ctccgcgaat tgcagcagag gattgctctg cgaaggaggc tcttcctcga
     5521 aaacgtcgag agccgcgccc gccacgcgac ccgccttcag ggcttcgtag agagcgcgct
     5581 cgtctatcaa tccgccgcgt gcgcaattga tgattcgcac ctccggcttc atgcgcgcaa
     5641 actcgcgtgc gccgatgatg ccgcgcgtct ccgccgtgag cggcgtgtgc accgtgagaa
     5701 agtcggcacg cgcgcagacc tcttcgagcg gcgcgagttc caactccatg tcgcgcgcct
     5761 gttcgggtgc gacaaacgga tcgaaggcca agaccttcat ttcaaagccg cgcgcacgac
     5821 tcgccactac gcgaccgata cgaccgaggc cgacgacgcc cagagtctta ccgcgcaact
     5881 ccacgccgac gaacttcttg cgctcccact tgccgccctg caaactcgcg tgcgcctgcg
     5941 gcacgcgacg ggcgagcgcg agcagcagcg cgagcgtgtg ctcggcggtc gtcatcgtgt
     6001 tgccgtcggg cgcgttcatc acgatgatgc cgcgcgtcgt cgccgccgcc acgtctatgt
     6061 tgtccacgcc gaccccggcg cgcccgatag cgcgcaggcg cacggcctcg tccatcaaag
     6121 ccgccgtcac cttcgtttcg ctgcgcacga cgagtccgtc gtagtcgcgc acgatctcgc
     6181 gcagttcgtc ggttttgagt ccggtcttct tttccaccgc gaagccggcg gcgcgcaaag
     6241 gttcaagccc gctctcgctc acgtcgtccg ataccagaat ttttatattg tccatgtagg
     6301 aggaactatg attcagcgtt caactcggaa agtgtcgcgg cgaaggcgca ggcatggaaa
     6361 gacacgctgc cacgcgtccg tctcgcaaca cttgttcggg gcgtcggaaa agttttaact
     6421 ttcgcgccgt cgccaacagt aatcgacgct ccacacgccg cccccggcca cggcgagata
     6481 gaggaacacg aagctgtaga gcgcggccag ttcgcctcga ttcaagaccg gccagagccc
     6541 ttgcggagca tgcgccatga agtaggccac cgccatcaag cccgacagga ggaacgccac
     6601 cgggcgcgtg aacaacccca gcagcaagag cagcccgccg aagaactcca gtattccgac
     6661 gaagcccatc atcgacagga gaggtggcgt ctgtgaaccc tgcgcccccg ccggaaagcc
     6721 gaacagtttt tgtgcgccgt gctgcatcat cagcaacgcc gcgacgatgc gcagcacgct
     6781 caagatacgc ggcgtccacg tcgagaaaaa cgaatcgtta gccatacaat tttgctcctg
     6841 tcattaagac ttttcgctgt cgccggagaa taacgcgcga cactctttca cgttggggcg
     6901 tagactttaa gcccgccgga cttttatctc tcgttccgta tccgcgaact ccacttctac
     6961 atcgggccgc cggagtctct cccatcggcg gagccgccgc gcgtcgtgtc gggcgtgccg
     7021 tcaggcgcgg gcgcggaaga cgaatcggat gcgccggaag tttcgccctg cgaggcggcg
     7081 gagtcagtct gagtttgttg caggtcgttc agggtgtcgc tgctcgtcgc ctcgtcttgc
     7141 cgcgctgcgg tctgtgcgct cttgtcgtcg ccgtttcccg cgccgggttt cgtccgttca
     7201 gcatcttcca ttttcttctc cttctaaaac agcaaatata gcgaactcag cagcgacatc
     7261 agcgtggcgg catagacgcc agcgagcacg tcgtcggcca tgatcccgag gccggcttcg
     7321 agcgattcga gacggcgaat cggatacggc ttccagatgt cgaacaggcg aaaggccaga
     7381 aagcccacga cgattgccca cgccccgatg ccgacgggca aaaacacgaa cgtcagaatc
     7441 tgcccgatca cttcatcaat gacaactgcg ccgggatctt tgcgcgccag cagcgactcg
     7501 caacgcgtgg ccgcccagat gcccgcaatc gtcatcacga tgagcatcag gagcacgacc
     7561 gtcgtacgca aagattcgag cggcggcgtg ctccatccgc gcgcctcggc gtacagaaac
     7621 gcctgcacac tcgccgcgcg cgccgcgaga tacaacccca cgccgaccaa cgatccccag
     7681 gttcccggcg cgagcggaat cagcccgacg ccgcacgtcg ccactccgag cgcgacataa
     7741 tcgcgcaaag ttcgctcacg ccgcgcacgc ccgatgaagg ccgtcgtcac caccaccgac
     7801 gggttcagtt catcgctcga ttctttaaca actttcataa ggcagtgaca agtgacaagt
     7861 tgaagccaag tgaaaactcg tcactcgtga atcatgaata gaagaaaaca gtctttctct
     7921 atttacgatt aacagttcac gttttacggc tctcttctat tcacgattca cgagtgacga
     7981 gtcacgtctc ttcaactcgt cacttgtcac tcgtcacttg tcacggctct atgatgcttc
     8041 cgacgagatc gtactcgtgt gcatctgtga tcaggacgtt gacgaagtcg ccgggcgcgg
     8101 gcgcgaagcc ttcgggcgcg tcgttgatca gaaccacgcc gtcaatgtcg ggcgcttgag
     8161 attcgagtcg cccctgccag agcaagtccg tctctttcgc cgcgccttca aagagaacgc
     8221 gcgcagttga accgacgcgc gcgcgattgc gcttgagcga gatgcggcgt tgctccttca
     8281 tcagacggtc gcgcctttta gcggcgactt tcggatcaac tttgtccggc agatcgtaag
     8341 cgggcgtccc ttcttcgtcc gaataggtga agacgccgac gcgatcaaac tctacgttcc
     8401 gcacgaaggc tagaagttcg ttgaaatctt cttcccgttc gccgggaaag cccgtgatga
     8461 acgtcgtgcg cacgccgatg cccggcacgc gcctgcgcac tcgttcgatc aggcgttcga
     8521 ggctcgcacg gtttcccccg cgacgcatca acttcaagac gttctgtgac gcatgctgca
     8581 aaggcatatc cagatacttg acggccttcg gctccgaggc catcacgtcg agaaagagat
     8641 cgctgatatg ggtcgggtag gtgtacatca cgcgcaccca ctcgatgccg tcgagtcttg
     8701 agagttcgcg gagcaggtgc gccagcccgt ccggctgttc gaggtcttcg ccgtagcggg
     8761 aggaatcctg cgcgacgagg atcaactcct tcacgccgcg cgcggcgagc gcctgcgcct
     8821 cgctcaccac ggagccgaag cgtcggctgc gaaaatggcc gcgcatctgc gggatgaaac
     8881 agaaagcgca cgggcggtcg cacccttcgg cgatcttgat gaacgcgctg tagccgggcg
     8941 tggcaagcac gcgcggcgtc gattcgtcgt agagataagt tgcgctttga ttgccgagcg
     9001 tgatgacggg cagcgagcgc gtgttaactt tgtcgtcgca aaccttcgtg atgtcgttga
     9061 tctggttcgt tccgatgaag gcgtcaactt cgggaatctc cgcgcgcaac tcgtcgcgat
     9121 aacgctcgac gagacaaccc gcgacgacga gccgcttagc cttccccgcc gtcttcatgc
     9181 gtgccgcttc gaggatcgca tcgacagact ctttcttagc cgaatcgatg aagccgcagg
     9241 tgttcaccac gagcgtttcg gcctccgcct cgtccgtcgt gatctcataa ccggcggcgg
     9301 cgagttgacc catcatcact tcgctatcaa ccagattctt cgggcagcct aaactgataa
     9361 agccgatctt cttcattcaa gtgacaggca acaggtgact ggtgacaagc aagaagtgat
     9421 gagtgatgag tgatgagtga tgagtcaaag atataacttt tgtttttaac tcattactca
     9481 ttactcatca ctcatcactt ttctttctcg tcattcgtca cctgttactc cctttccttc
     9541 tctatgcgcc gcagccattc ttcgatgcgg gcggcttccg ctgtcgttag ttcgtgcgcg
     9601 ccgaagcgta cgcggttata ggagcgcgtc agcagcgtcg cttcgggcat gcccgtcgcc
     9661 gcagcgaatt cgagcggcgt ctcgtcggga gcgcgcaaga ggccgcgcga ttcgagcgct
     9721 tttgtcatgc gttcgtaaaa gacaacggat gaataatttt tgccttcgcg ccgccagaag
     9781 ttgaggccac gcctgaagtc ccaacggcgg acgcgcgcga cgaccattaa gagcagtccg
     9841 gcgagaagga cgagcgcgac gacgacagga agattcgtcg cccggatcaa gccctgtgcg
     9901 tcgccgccgt tcgttacctg tcgccaccag cctgacagtt cggcgcgcag ttcggcgaaa
     9961 gtttgcccca ccgtctgccg gtagacgccg aattggccgc gcacagatgt cgctagtgaa
    10021 cgctgttcct gcttatcata agcgacgaca tattgaatcc aaaacagttc aagggcttcc
    10081 gcatacttgc cgagtccgcc gcgccagaaa cccgtctcat cattattgcc gggtcgcccg
    10141 gccacaggag tcgggtcgaa agtgacccag gcgtttgtct ccgggaaata gacctcgacc
    10201 cacgagtgcg cgtccgattg cctgacggtg tagacatccg ccgcgtcgtt gtagtcgccc
    10261 aactgaaaac cgttgacgac gcgcgcggcg atgccctcgc tgcgcagcat gacggccata
    10321 gcggtcgaga aatattcgca atggccttcg cggacacgaa acatgaaatc ggccagcggg
    10381 tcgcttgcgc cgcccgattt ctgctccaac gtataacgaa agttctgttg aaaatatgct
    10441 tcgatgacgc gcgcggcgtc gtaacgattg tctgagccgg tgccgactat caggccgcgc
    10501 gtgagttggc ggatgcgcgg gtcgaggtcg tccggcactt tctggtagcg atcaaaggtg
    10561 cgcgggtagg gcgcgaggt
//
LOCUS       DASWNS010000505        12274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_252651, whole genome shotgun sequence.
ACCESSION   DASWNS010000505 DASWNS010000000
VERSION     DASWNS010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12274
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252651"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="VGB76_17425"
     CDS             complement(<1..1560)
                     /locus_tag="VGB76_17425"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0100731.1"
                     /translation="MIRIRNFCLAFVVFALASGNSIAALAQTTNRTATGAAAPMVAPR
                     LDFEKYKLKNGLEVILLKDDRLPLVATNLWYHVGPANERPGLTGFAHLFEHMMFQGSK
                     HVGDDAHFKLLEGAGANDINGTTDFDRTNYFETLPSNQLELALWLESDRMGFLLDTLD
                     AARLANQRDVVRNERRQGENSPYGLVEEAVYQNLFPKSHPYYAWVIGSHADIESARLK
                     DVREFFKTYYAPNNASLAIVGNIDKAQTKTLVEKYFGSIPAGPPVPKINVTTPPITSE
                     RRVVVTDQVELPRIYMAWITDPIYKPGDAEADLLAMILGGGKSSRLYKRLVYERQIAQ
                     DVSVSQNSLILGSVFQITATAKPGVKPEDLERAINEELDAVRKEGVTADQLSRARNVV
                     QTRIVQGLETLGGFGGVADRLNQYNHYLGDPGFLAKDLERYDRATAAGLQKVAQEKLT
                     NNSRVVVYGVPGKKVVDDVPKTTEAEATAEASATPASGNTAAQQSDDSWRNRQPQAGA
                     ASKLALPVPKSF"
     gene            1858..2793
                     /locus_tag="VGB76_17430"
     CDS             1858..2793
                     /locus_tag="VGB76_17430"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0100732.1"
                     /translation="MAASQMPVKNRVARLICLVLCAFAPGAEVASARVAPERLSIAVL
                     DLGETETGRRVADELSAALAKTSNLDPVNRAQARAAARGTGYTGSLNLTLTEARDLGD
                     AIGCDFYFTGEAQTLRRTSSARADYFESYAALFLVSATTGRLLLWEHFNAESATHAEA
                     EKSLLAELRARAARYGETIHAARESERRERQLARERAEAAAFEDVPDEGSPAALNFRA
                     PQPYRSLRPSYPETAARVAAEATVDALVKVDAEGEVKDIEIVRWAGYGLNEAVIATVR
                     QLHFRPATRDGRAIPIRVLLRYNFRRPVEENKGKR"
     gene            2812..4164
                     /locus_tag="VGB76_17435"
     CDS             2812..4164
                     /locus_tag="VGB76_17435"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0100733.1"
                     /translation="MRQFPSRALRAPGYRALPCVLFLLLLLSSFPFSSSSSARERVTT
                     ATREGRLIVFDETWRMIRERYYDPALHGVDWDELRARLRPLAAEARGETEFYTVLRRL
                     TSRLRDAHTRVFAPDERFDWQRPTYISVGVSLREIAGEIVVTGVERESEAEQGGVRAG
                     DELLEIEGEAVESVFARSLAEEVGASTDVTARQHAAARLLRGARDTFVTALFETNNGR
                     ATKKIRLRRVLRNRPPALRVQKAESFPVVNFNIFVPEIASELMRAFRRGELRTARGLI
                     IDLRDNGGGQAETMTDIASAFLPQGTSLGAFTDREGRIASTPQTRAAMLFAADAVAEF
                     RGAVVVLTSTRTASAAEILAAALQESKRAKIIGERTCGCVLAIRRRHTLPDGGLLDIS
                     EMDFRTARRTRLEGTGITPDEFVSPTRRDLREGRDPALERASKLLREAMRGETSVGQR
                     "
     gene            4435..5670
                     /locus_tag="VGB76_17440"
     CDS             4435..5670
                     /locus_tag="VGB76_17440"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0100734.1"
                     /translation="MLYLSQVLRRPVRDVEGRPVASIKDVIVHLGEDHPPVTGLVARH
                     ARRLIYLPRTRIARFGEAGAQLNFDLLDLRDFKRRDGEVLLARDVLDKQLIDVDGKRV
                     VRVNDVQLIEVAGEWRVTGADVSLQGLWRRLAPAGFFGSRRPVEVIDWADVGYLATDT
                     TEVRLKSSRDKLSRLHPVEIARLAEALSYRQGAEIVAALDDETAAETLEEMHDENQVR
                     IIGDMDKERAADILEWMSPDEAADVLGDLPEATAEDLLSRMEDEEKTDVAELLHYEDD
                     TAGGLMTSEFITLPQGLTVGEAIARLREMAETPNMIYYLYVVESEGSWKLSGVIALRS
                     LILADPAAPLEHVMRDEFQHAHPDDSAKEVAHKIAEYNLLALPVVDDLGEIAGIVTVD
                     DAMELLLPKGWRQRLPRIF"
     gene            5708..6070
                     /locus_tag="VGB76_17445"
     CDS             5708..6070
                     /locus_tag="VGB76_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100735.1"
                     /translation="MTRTEWLIWVLRYGDPKYRYKGIREAIKFELYSLWWRVKGEKLT
                     HWLNEKPKPAGKYILTKSLPRIGFKIFGQTFCFSEEMPVEYVPYTPELARKLQAQRGK
                     PVKVVEINYVLGGRADGF"
     gene            6128..7417
                     /locus_tag="VGB76_17450"
     CDS             6128..7417
                     /locus_tag="VGB76_17450"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY0100736.1"
                     /translation="MSRRLWRRGTLRVRRTMERRTGFIAYLAVIGPGMVAANAGNDAG
                     GIATYSTAGAEFGYTLLWTFVPILVSLGLVQEMCARMGVVTGKGLADLIRERFGVRLT
                     MVFMAALLVANAGVTVSEFVGIAAATELFGVPRQISVPLAAFSVWWLVVKGSYRRVER
                     VFLVMSLVFLGYIVSAFMARPDWGEVARQMAHPAFHYDSHYLFTLVALIGTTISPYMQ
                     VFVQSSVVEKGVRKEDYKLVRADVWVGTIFSMSIAFFIVISTAATLNASGFTGGIETA
                     ADAATALVPLAGQYAGLLFAIGLFGASMLAAGVLPLATAYSISEALGFEKGVSRSFRE
                     APIFIGIFTFLIVVGALIAILPGLPLMRVLVVTQFINGLLLPIMLIAVLRLAGDRTLM
                     GDNVNGAVYNVLAWLMVLVVSGLSLLLVGKTLFGGAP"
     gene            7512..8978
                     /locus_tag="VGB76_17455"
     CDS             7512..8978
                     /locus_tag="VGB76_17455"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY0100737.1"
                     /translation="MSSSRLKKQLLWPLLATALCALLLFLAVPAFAPPALSDEGLRAL
                     SESQRAEHSRAATVYYWMSTLAKIALLLSLMFVAVRLINAFLFDLVFRLRKGREAPSL
                     IRDLFSIVAYVLIVAFILKHYFALSLGALLSGSALLGVILGLALQDTLGNLFSGISLH
                     ADKPFEVGDFITVGKYAGVIESVTWRAAKIRTLNNNVVLLSNSQIAKEAIEVYPRDNP
                     NARLVFFNALYSHSPVKVIEVVRNAIRETENVRRSPAPVIRIRNLGDFAVDYEIKYWA
                     EDYVLQNDTDALIRQRIWYAFKRAGLSFAYPTQTLYIERPTPAASRAADEETLTGRLS
                     ATGIFAPLNPDELSQLACASRSHTYAPGETIIRAGDRGDSMFVVHHGHVAVQVTDNGQ
                     PRTVATLGEGDFFGEMALLTGEPRSAHVVAAEETEVLEIGHEAMKHLFDTNPRLAESL
                     GETIAHRRAGLAASAANEGVAEVSAGVISSIKRFFRLD"
     gene            9189..9734
                     /locus_tag="VGB76_17460"
     CDS             9189..9734
                     /locus_tag="VGB76_17460"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0100738.1"
                     /translation="MSITRYDPFRDLRMLQDEVNRLFSSNLSRSFSDEGIARGAWAPT
                     VDIYENKDHIVLEAELPGMNREDFELSIENNVLTLRGERRFEKKDESDNYHRVERSYG
                     SFTRSFTLPQTVSPENVTAEYKNGVLRVVLQKREEVKARRIEIAGEGAGGNAQPKTIE
                     AKADEPGASEGKGKTATAGGR"
     gene            9840..>12274
                     /gene="clpB"
                     /locus_tag="VGB76_17465"
     CDS             9840..>12274
                     /gene="clpB"
                     /locus_tag="VGB76_17465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY0100739.1"
                     /translation="MRLERFTLRGQEAIQTAIEQAERNQNQQVEPEHLLVAMLEQPEG
                     IVRPLLGKLGANLQVVLNDANAAIGRLPKVQGGQQYFSPRLTQIFTAAQKQAETMQDE
                     YISTEHLLLAIVNEKDGAAGRILRQHGVNRDDLTKVVSEMRGDSRITDQNAESNYQAL
                     SKYAKDLTDLARQGKLDPVIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIISGDVPETLRNKRLVALDLGSMLAGAKYRGEFEDRLKAVLKEIENAKGQIVLFI
                     DELHTLVGAGAAEGAIDASNMLKPALARGELRCVGATTLNEYKKHIEKDAALERRFQQ
                     VYVGEPTVEDTIAILRGLKERYEVHHGVRIKDSAIVAAATLSNRYITDRFLPDKAIDL
                     IDEAASRLRIEIDSLPVEIDELERETTGLEIEKQALQRETDERSQARLREIEVKIADV
                     RERASGMKAQWRSEKDIIERIRKAKEELEQIKVQAEQYERAGDYGKVAELRYGTAVEL
                     QKELDADHAKLAEIQTAGVYLKEEVDEEDIALVVAKWTGVPVAKMLEGEMQKLVTMEE
                     RLRLRVIGQDEALTAVANAVRRARAGLQDPNRPVGSFIFLGPTGVGKTETARALAEFL
                     FDDERAMIRLDMSEYMEKHAVARMIGAPPGYVGYEEGGQLTEAVRRRPYSVVLFDEIE
                     KAHPDVFNVLLQILDDGRLTDSKGRTVDFKNTVLIMTSNLGSREIQAAGDDEKLVREA
                     VLEELRGHFKPEFLNRIDDVVIFHQLSRAEIVQIIEVQLERLRHMLQERDITLALDDS
                     ARELLAREGYDPM"
BASE COUNT         2329 a         3751 c         3909 g         2285 t
ORIGIN      
        1 gaaggatttg ggaacgggga gcgcgagttt ggatgcggct ccggcctgcg gctggcggtt
       61 gcgccaagag tcgtccgact gttgcgccgc agtgttgccg ctcgcaggcg tcgccgaagc
      121 ctccgccgtc gcctcggctt cggtcgtctt cggcacgtcg tccacgacct tctttccggg
      181 cacgccatag acgacgacgc gcgagttgtt cgtcagtttc tcctgcgcca ccttctgcaa
      241 accggcggcg gtcgcgcggt cgtaacgctc taaatctttc gcgaggaaac cggggtcgcc
      301 gaggtaatgg ttgtactggt tgaggcggtc ggccacgccg ccgaagccgc cgagcgtctc
      361 caagccctgc acgatgcgcg tctggacgac gttgcgtgcg cgagagagtt gatcggcggt
      421 cacgccctct ttgcgcacgg cgtcgagttc ttcgttgatg gcgcgttcga ggtcttcggg
      481 tttgacgccg ggcttcgccg tcgccgtaat ctgaaagacc gagccgagga tgagcgagtt
      541 ctggctgacg ctgacatcct gcgcgatctg ccgttcatag acgagccgtt tgtagaggcg
      601 gctcgatttg ccgccgccga ggatcatcgc cagcagatcg gcttcggcgt cgccaggctt
      661 gtagatcgga tcggtgatcc aggccatgta gatgcgcggg agttcgacct gatccgtgac
      721 gacgacgcgc cgctcggaag tgatgggcgg cgtggtcacg ttgatcttcg gcacgggcgg
      781 tccggcgggg atggaaccga aatacttttc gaccagcgtt ttcgtctgcg ccttgtctat
      841 gttgccgacg atggcgaggc tcgcgttgtt gggcgcgtag taggtcttga aaaactcgcg
      901 cacgtccttg agtcgcgccg attcgatgtc ggcgtgcgag ccgatgaccc atgcgtaata
      961 aggatgcgac ttcgggaaga ggttttgata gaccgcttct tcgacgaggc cgtaggggga
     1021 gttttcgccc tgacggcgtt cgttgcgcac cacgtcgcgc tggttggcga ggcgtgcggc
     1081 gtcgagcgtg tcgagcagaa atcccatgcg gtcgctctcc agccagagcg cgagttcgag
     1141 ttggttggag gggagcgtct cgaaatagtt ggtgcggtcg aagtcggtcg tgccgttgat
     1201 gtcgtttgcg cccgcgcctt cgagcaattt gaagtgggcg tcgtcgccga cgtgcttcga
     1261 gccttgaaac atcatgtgct cgaagaggtg tgcgaaacct gtgaggccgg gacgctcgtt
     1321 ggcaggtccc acgtgatacc acaaattggt ggcgacgagc ggcaggcgat catctttgag
     1381 caggatcact tcgaggccgt tcttcagttt atacttctcg aaatcgaggc gcggggcgac
     1441 catcggagcg gcggcgcccg tcgccgtgcg attggtggtt tgcgcgagcg cggcgattga
     1501 attgccggag gcgagcgcga agacgacaaa cgcgaggcaa aagtttctga tgcgaatcat
     1561 aaagcgttac tccctgacgc gggcttcgat tgcagcccgc gccgctcaag cgtagttgga
     1621 attcgtgttg ccgcgctctg gcagaagaag cgcggaagag tctgctggtt gtagagcctg
     1681 cggcgcggtt ggacgcggaa gaccggcttc tcgcgaggcc acgcgccgga atgatgctaa
     1741 acttttattg agtcgaatcg aatttggagg cgggcgtccg cgtgcgctga aacgcccctg
     1801 ccgttcgtcc tgacaaaccc ttgtttcatt cggaatcaat ccgcttgtac cgcaaagatg
     1861 gcagctagtc aaatgcccgt taagaatcgc gtcgcgcgat tgatctgcct cgtgctgtgc
     1921 gccttcgcgc cgggcgcgga ggtggcgtcg gcgcgcgtcg cgccggaacg cctgagcatc
     1981 gccgtgctcg atctcggcga gacggagacg ggacgacgcg tggctgacga actctccgcc
     2041 gcactcgcca aaacgtcgaa cctcgatccg gtcaatcgcg cacaggctcg cgccgccgca
     2101 cgcggcacgg gctacacggg ttcgctcaac ctgacgctca cggaggcgcg cgatctcggc
     2161 gacgccatcg gctgcgattt ttacttcacg ggcgaggcgc aaaccctgcg tcgcacgtcg
     2221 tccgcgcgcg cggactattt cgagtcctac gccgcgctct ttctcgtcag cgcgacgacg
     2281 gggcggctcc tcctgtggga acacttcaac gcggaatcgg ccacgcacgc cgaagccgaa
     2341 aagagcctgc tggccgaact gcgcgcacgc gccgcgcgct acggcgagac gatccacgcg
     2401 gcgcgagagt ctgaaagacg cgagcgacaa cttgcgcgcg aacgtgcgga agccgccgcg
     2461 tttgaagatg tgccggacga gggcagcccc gccgccctga actttcgcgc gccgcaaccc
     2521 tacaggagcc tgcgcccgtc ctatcctgag acggcggcgc gcgtggcggc ggaagccacc
     2581 gtggatgcgc tcgtcaaggt tgacgcggag ggcgaagtca aagatattga gatcgtgcgc
     2641 tgggctggtt acggtctgaa cgaagccgtg atcgcgacgg tgcgccaact acactttcgc
     2701 ccggccacgc gcgacgggcg agccattccc atacgcgtgc tgctgcgtta caacttccgg
     2761 cgaccggtgg aagagaacaa gggtaaaagg taaaagggaa gggaaaagac tatgcgacag
     2821 tttccatctc gcgctctacg cgctcccggc tatcgtgcgc ttccctgcgt gctgttcctt
     2881 ctccttctcc tttcctcttt ccccttttct tcttcttctt ccgcacgcga gcgcgtgacg
     2941 acggcgacgc gcgagggacg cctcatcgtc ttcgacgaga cgtggcggat gatccgcgag
     3001 cgttattacg acccggctct gcacggcgtt gactgggatg agttgcgcgc acgcctgcgc
     3061 ccgctcgccg cagaggcacg cggggagacg gagttctata cggtgctgcg ccgcctgacg
     3121 agccgcctgc gcgatgcaca cacgcgcgtc tttgcccccg acgaacgctt cgattggcaa
     3181 cgcccgacct acatcagcgt cggcgtctcc ctgcgcgaga tcgcgggtga gatcgttgtc
     3241 acgggagtcg aacgcgagag cgaggccgag cagggcggcg tgcgcgcggg cgacgagttg
     3301 ttggagatag agggcgaagc ggtcgagtcc gtcttcgcgc gcagtctggc ggaagaggtc
     3361 ggggcatcga ccgacgtgac ggcgaggcaa catgccgccg cgcgcctgtt aagaggtgcg
     3421 cgcgacacgt ttgtcacagc cctcttcgag acgaacaacg ggcgcgcgac aaagaagatt
     3481 cgcctgcgtc gcgtgctcag aaaccgcccg ccagcgttgc gcgtgcaaaa ggcggagagt
     3541 tttccggtcg tcaatttcaa catcttcgtg ccggagatcg ccagcgagtt gatgcgcgcg
     3601 ttccgtcgcg gcgaactgcg cacggcgcgc ggactcatca tcgacctgcg cgataacggc
     3661 ggcggtcagg ctgagacgat gaccgacatc gcctccgctt ttctcccgca ggggacgagc
     3721 ctcggcgcgt tcacagaccg cgaagggcgc atcgcgtcca cgccgcagac gcgcgccgcg
     3781 atgctctttg ccgcagacgc cgtggcggag tttcgcggcg cggtcgtcgt cctgaccagc
     3841 acgcgcacgg cgtctgcggc ggaaattctc gctgccgctt tgcaggagtc gaagcgcgcg
     3901 aagatcatcg gcgagaggac gtgcggctgc gtgctcgcca tccgccgacg ccacactttg
     3961 cccgacggcg gcctgctcga catcagcgag atggacttcc gcaccgcccg ccgcacgcgg
     4021 ctcgaaggca caggcatcac gcccgacgaa ttcgtcagcc ccacccgccg cgacctgcgc
     4081 gaagggcgcg accccgcgct cgaacgcgcc agcaaacttt tgcgcgaagc gatgcgcggc
     4141 gaaacaagcg tcggccagcg atagacacag cggcgataga cagagccgcg atagacgagg
     4201 cgacgataga cgaggcgacg acaggcagtg cggcgacagg gagtgcggcg atagatagtg
     4261 cgacgagagc taggccgcag cgttcctgtc cgggcgttgg cgtttgtcgc cttcgcctgc
     4321 gtccagagct ttttacctct gcgtcgccgc aatcttttag cttttcgtcc cgcgccgttt
     4381 gcgcccgttt ctttttcgct tccccttttc cgcttcccct tttaatatcc gtccatgctc
     4441 tatttatcac aggtgcttcg tcgccccgtg cgcgacgtgg aaggccgccc tgtggcctcg
     4501 atcaaggatg tcatcgtgca tctgggcgaa gatcatccgc ccgtcaccgg actcgtggcg
     4561 cgccacgcac ggcgtctgat ctacctgccg cgcacgcgca tcgccagatt cggcgaagcg
     4621 ggcgcgcaac tcaacttcga cctgttagat ctccgcgact tcaaacgacg cgacggcgaa
     4681 gtgctgctcg cgcgcgacgt gctcgacaag cagttgatcg acgtggacgg caaacgcgtc
     4741 gtgcgcgtca acgacgtgca gttgatcgaa gtggcgggcg agtggcgcgt gacgggcgcg
     4801 gacgtgagct tgcagggact ctggcgcagg ctcgctcccg ctggcttctt cggcagccgt
     4861 cgccccgtcg aagtgatcga ttgggcggac gtgggttacc tggcgaccga tacgacggag
     4921 gtgcgcctga agtcttcgcg cgataagttg tcgcgcctgc acccggtgga gatcgcgcgg
     4981 ctcgccgagg ctctctctta ccggcagggc gcggagatcg tcgccgcgct cgacgacgaa
     5041 accgccgccg agactctgga agagatgcac gacgaaaatc aggtgcgcat catcggcgac
     5101 atggacaagg aacgcgccgc agacatcctc gaatggatgt cgcccgacga agcggcggac
     5161 gtgctcggcg acctgcccga agccaccgcc gaagatttac tgagccgcat ggaggacgag
     5221 gagaagacgg acgtggccga acttctccac tacgaagacg acacggcggg cggcctgatg
     5281 acgagcgaat tcatcacgct cccgcagggc ttgacggtcg gcgaagccat cgcacgcctg
     5341 cgcgagatgg ccgaaacccc gaacatgatc tattacctct acgtcgtcga aagcgaaggc
     5401 tcctggaaac tgtccggcgt gatcgccctg cgcagcctca tcctcgcaga ccccgccgcg
     5461 ccgctcgaac acgtcatgcg cgacgagttc cagcacgcgc accccgacga ttccgcgaaa
     5521 gaagtggcgc acaagatcgc cgaatacaac ctgctcgcgc tgcccgtcgt tgacgacttg
     5581 ggagagattg ccgggatcgt tacggttgac gacgcgatgg agttgttgct gccgaaaggc
     5641 tggcgacaac gcctgccaag aattttctga aatttaactt cggcctttgt ccgtagcggg
     5701 tgtggttatg acgagaacgg aatggctcat ctgggtattg cgttacggtg atcctaaata
     5761 tcgctacaag ggcatcaggg aagccatcaa gtttgaattg tatagtctgt ggtggcgtgt
     5821 gaaaggtgaa aaactgacgc actggttgaa tgagaagccg aaacctgccg gaaagtatat
     5881 cctgacgaag tcgctgccgc gcatcggttt caagattttc ggtcagacgt tttgctttag
     5941 tgaagaaatg ccggtcgaat atgttccata cacgccggaa cttgcccgta agttgcaagc
     6001 gcagagaggc aagcctgtga aggttgtaga gatcaactat gtgctcggcg gtcgcgccga
     6061 tggattttaa tgcagatgcc tgacggagaa cctccaagtg gccgtgtcgc gtgcagatgt
     6121 gcgggagatg agtcgtcgcc tgtggcggcg cggcactctg cgcgtgcggc gcacgatgga
     6181 gcggcggacc gggttcatcg cgtacctcgc agtcatcggg ccggggatgg tcgcggcgaa
     6241 cgcgggcaac gacgcgggcg gcatcgccac ttattcgacc gccggggcgg agttcggcta
     6301 cacgctgctc tggacgttcg tgcccatcct cgtcagcctc ggactcgtgc aggagatgtg
     6361 cgcgcgcatg ggcgtggtga cgggcaaggg cttggccgac ttgatccgcg agcgtttcgg
     6421 cgtgcgctta acgatggtct tcatggccgc gctgctcgtg gcgaacgcgg gcgtcaccgt
     6481 ctcggagttt gtcggcatcg cggcggcgac ggagttgttc ggcgtgccgc gccaaatctc
     6541 cgtgccgctc gccgcgttct ccgtctggtg gctcgtcgtg aagggttcgt atcgcagggt
     6601 cgagcgcgtc tttctcgtca tgtcgctcgt ctttttgggc tacatcgtct cggcgtttat
     6661 ggcgcgcccg gactggggcg aagtggcgcg gcagatggcg caccccgcct tccattacga
     6721 tagccattac ctgttcacgc tcgtcgctct gatcggcacg acgatctcgc cctacatgca
     6781 ggtcttcgtg cagtcgtccg tggtcgagaa gggcgtgcgc aaagaagact acaaactcgt
     6841 gcgcgcggac gtgtgggtcg ggacgatctt ctccatgtcc atcgccttct tcatcgtcat
     6901 ctcgacggcg gcgacgctca acgcgagcgg ctttacgggc ggcatcgaga cggcagccga
     6961 cgccgcgacc gcgctcgtcc cgctcgcagg gcaatacgcc ggactcctct tcgccatcgg
     7021 actcttcggc gcgtcaatgc tcgccgcggg cgtgttgccg ctcgccacgg cctattcgat
     7081 cagcgaagcg ttggggttcg agaaaggcgt ctcgcgcagt tttcgcgaag cgccgatctt
     7141 cataggcatc ttcaccttcc tgatcgtagt cggcgcgctc atcgcgatcc ttccggggtt
     7201 gccgctgatg cgcgtgctcg tcgtcacaca gtttatcaac ggcctgctgc tgccgatcat
     7261 gctcatcgcg gtgttgcgcc tggcgggcga ccgcaccttg atgggtgata acgtgaacgg
     7321 ggcggtctat aatgtgctgg cgtggttgat ggtgctcgtc gtctccggcc tgtcgctcct
     7381 gctggtcggg aaaaccctgt tcggcggcgc accctgaaca gtcgcccgta gcagtccggg
     7441 ttatagtcaa gagcctttcg cctgatcctt caattcgcgc tttcccggcg actaacgtgt
     7501 aacgcttcaa gatgagtagc tcccgcttaa aaaaacaact tctctggccg ctactggcaa
     7561 ccgcactctg cgccctgttg ctatttctag ccgtcccggc cttcgcgccg cctgcgctga
     7621 gcgatgaagg attgcgcgcg ctctccgagt cgcagcgcgc cgaacacagc cgcgcggcca
     7681 ccgtctatta ctggatgagc acgctggcga agatcgctct gctgctgagc ctgatgttcg
     7741 tggccgtgcg tctcattaac gcattcctat tcgatctcgt ctttcgcctg cgcaaaggca
     7801 gggaagcgcc gagcctcatc cgcgacctct tctccatcgt cgcctatgtg ctcatcgtcg
     7861 ccttcatcct caaacactac ttcgcactct cgctcggcgc gctgctctcc ggctcggcct
     7921 tgctcggcgt catcctcggc ctcgccttgc aggacacgct cggcaacctc ttctccggca
     7981 tctcgctcca cgcagataag cccttcgagg tgggcgattt catcacggtc ggaaaatatg
     8041 cgggcgtcat cgaaagcgtg acgtggcgcg cggcgaagat cagaacgctc aataacaacg
     8101 tcgtgctcct cagcaacagc cagatcgcga aagaggccat tgaggtctac ccgcgcgaca
     8161 atccgaatgc gcgcctcgtc ttcttcaacg cgctctactc acactcgccc gtcaaagtga
     8221 tagaggtcgt caggaacgcc atacgcgaga cggaaaacgt gcgccgcagt cccgcccccg
     8281 tcatacgcat acgcaatctg ggcgacttcg ctgtggacta cgagatcaag tattgggcgg
     8341 aagactacgt gctgcaaaac gacaccgacg cgctcatccg ccagcgcatc tggtatgcct
     8401 tcaaacgcgc cgggctgagc ttcgcctacc cgacgcagac gctctacatc gagcgtccga
     8461 cgcccgccgc gagccgggcg gcagacgaag agacgctcac agggcgtctc tccgccacgg
     8521 gcatcttcgc cccgctcaac ccggacgagc tttcgcaact cgcctgcgcc tcccgcagcc
     8581 acacctacgc gcccggcgaa accatcatcc gcgcgggcga ccgtggcgat tcgatgttcg
     8641 tcgtgcatca cggccatgtc gccgtgcagg ttacggataa cggccagccg cgcaccgtcg
     8701 ccacgctcgg cgagggagat ttcttcggcg agatggctct gctcacgggc gaaccgcgct
     8761 cggcgcacgt cgtcgccgcc gaagagacgg aggtgctcga aatcggccac gaagccatga
     8821 agcacctctt cgacaccaac ccgcgcctgg ccgaatcgct cggagaaacc atcgcgcatc
     8881 gccgcgcagg gctggccgcg tcagccgcaa acgaaggcgt ggcagaggtt tcggcaggcg
     8941 tcatctcttc cattaaacgc ttcttcagat tggattaagc ggattttcgc gcccctcttc
     9001 ccgccgccgt atccggcatg cgaaaaaact cgaaaatttt tctcgacggt acttgacaag
     9061 aagagactca gatattatta ttctcgcacg ctcaaaggtc ggggtcgcca gttgaaggtt
     9121 ttaaatccgg cgggagcctc gcgggagcgt ccagcaaata acgtttttgt tgaggaggaa
     9181 attacgtcat gtctatcacc cgttacgatc cgttccgcga tttgcggatg ctgcaagacg
     9241 aagttaatcg cctgttctcc agcaacttgt cccgctcctt cagcgacgag ggaatcgcac
     9301 gcggcgcgtg ggcgccgacc gtggacatct acgagaacaa agaccacatc gtgctcgaag
     9361 cagaacttcc gggcatgaac cgtgaagact ttgaactctc aatcgagaac aacgtgctca
     9421 cgcttcgcgg cgagcgtcgc ttcgagaaga aggacgaatc ggacaattat catcgcgtcg
     9481 aacgctctta cggttcgttt acgcgctcgt tcacgctgcc gcagaccgtc tcgccggaga
     9541 acgtgaccgc cgagtacaaa aacggcgtgc tccgggtcgt gctgcaaaag cgtgaagagg
     9601 tgaaagcgcg tcgcatcgag attgcgggcg agggcgcggg cggcaacgcg cagccgaaga
     9661 ccatcgaagc gaaggccgat gagccgggcg cttcggaggg caaaggcaag actgcgacag
     9721 caggcggcag gtaatcggtt taacaattcg gtaacagcca ggcgcgcgga acgatgagcg
     9781 gtgaactcat tcatcgttcc gcgcttcgca ccttatagac gattcaggat ttcaggaata
     9841 tgcgtttaga acgttttact ctgcgcgggc aagaggccat ccagacggcc atcgaacagg
     9901 ccgagcgcaa ccaaaatcaa caggtcgagc cggaacacct gctcgtcgcc atgctcgaac
     9961 agccggaagg tatcgtgcgc ccgctgctcg gcaaactcgg cgcgaacttg caggtggttc
    10021 taaacgatgc aaacgccgct atcggtcggc tgccgaaagt gcagggcggg cagcagtatt
    10081 tcagcccgcg cctgacgcag attttcaccg ccgcgcagaa gcaggccgaa acgatgcagg
    10141 acgagtacat ctcgaccgag cacctgttgc tggcaatcgt gaacgaaaag gacggcgcgg
    10201 cgggtcgcat cctgcgccag cacggcgtca accgcgacga cctgacgaaa gttgtcagcg
    10261 agatgcgcgg cgattcgcgc atcacagacc agaacgcaga gtcgaactat caggctctct
    10321 cgaagtacgc gaaagacctg acagacctcg cgcggcaggg caaactcgat cccgtcatcg
    10381 ggcgcgacga cgaaatccgc cgcacgattc aggttctctc gcgacgcacg aaaaataatc
    10441 ccgtgctcat cggcgaaccg ggcgtcggca agacggccat cgtcgaggga ctcgcgcagc
    10501 ggatcatctc cggcgacgtg ccggagactt tgcgcaacaa gcgtctcgtc gcgctcgacc
    10561 tcggctcgat gctcgcgggc gcgaagtatc gcggcgagtt tgaagatcgc ctgaaggctg
    10621 tcttgaagga gatcgagaat gcgaaggggc agatcgtcct tttcatagac gaactgcaca
    10681 cgctggtcgg cgcaggcgcg gctgaaggcg cgattgacgc gtcgaacatg ttgaagcccg
    10741 cgctcgcacg cggcgagttg cgctgcgtcg gcgcgacgac tttgaacgag tacaagaagc
    10801 acatcgaaaa agatgccgcg ctcgaacgcc gcttccagca ggtttacgtg ggcgagccga
    10861 ccgtggaaga cacgattgcg atcctgcgcg gtttgaagga acgctacgaa gtgcatcacg
    10921 gcgtacgcat caaggactcg gccatcgtcg cggcggccac gctctccaac cgttacatca
    10981 cggatcgttt cctgcccgac aaagcgattg acttgattga cgaagcagcc tcgcgccttc
    11041 gcatcgagat cgattcgttg cccgtcgaga tcgacgagtt ggagcgcgag acgaccgggc
    11101 ttgagatcga gaagcaggct ttgcagcgcg agacggacga gcgttcgcag gcgcgcctgc
    11161 gtgagatcga ggtcaagatc gcggacgtgc gcgaacgcgc ttcgggcatg aaggcgcagt
    11221 ggcgttcgga gaaggacatc atcgagcgca tccgcaaggc caaagaggaa ctcgaacaga
    11281 tcaaggtgca ggccgagcag tacgagcgcg cgggcgacta cggcaaggtc gccgaactgc
    11341 gctacggcac ggcggtcgaa ctgcaaaagg aactcgacgc ggatcacgcg aagctcgcgg
    11401 agatacagac ggcgggcgtc tatctcaagg aagaggttga cgaagaggac atcgctctgg
    11461 tcgtcgccaa atggacgggc gttcccgtcg ccaagatgct cgaaggcgag atgcagaaac
    11521 tcgtcacgat ggaagaacgc ctgcgcctca gagtcatcgg gcaggacgag gctctaacgg
    11581 cggtcgccaa cgccgtgcgg cgtgcgcgtg cgggcttgca agacccgaac cgtcccgtcg
    11641 gctcgttcat cttcctgggt ccgacgggcg tggggaagac cgagacggcg cgtgcgctcg
    11701 cggagtttct cttcgacgac gaacgcgcga tgatccggct cgatatgtcc gagtacatgg
    11761 agaagcacgc cgtggcgcgc atgatcggcg cgccgcccgg ctacgtcggc tacgaagagg
    11821 gcgggcagtt gaccgaggcc gtccggcgca ggccgtacag cgtcgtcctc ttcgacgaga
    11881 tcgagaaggc gcacccggac gtgttcaacg tgctgttgca aattttggac gacgggcgtt
    11941 tgaccgactc gaaggggcgc acggttgatt tcaagaacac ggtcttgatc atgacctcga
    12001 acctcggctc acgcgagatt caggcggcgg gcgacgacga gaaactcgtg cgcgaagcag
    12061 tgctggagga actgcgcggc cacttcaaac cggagtttct caaccgtata gacgacgtgg
    12121 tgatcttcca ccaactctcg cgcgcagaga tcgtgcagat catcgaagtg caactcgaac
    12181 gcctgcgcca catgctgcaa gagcgcgaca tcacgctcgc gctcgatgat tcggcgcgcg
    12241 aactgctcgc acgcgagggc tacgatccga tgta
//
LOCUS       DASWNS010000506         6699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_44863, whole genome shotgun sequence.
ACCESSION   DASWNS010000506 DASWNS010000000
VERSION     DASWNS010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44863"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1248)
                     /locus_tag="VGB76_17470"
     CDS             complement(190..1248)
                     /locus_tag="VGB76_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY0100740.1"
                     /translation="MLEEKNQVTSNKPELNKNESVNAKTGAIGLDIGTSRIVMADSSS
                     AKDSRSQLNAFVAVPSSEMAENVLKHKNMVYERNCKNLYVYGNDSNFFASFLNVDARR
                     PMRDGLLNAQEEMSQHIMQLIIQRIVPRGRGKEMLYFSVPGKGEGVNGKLVYHEAVLR
                     SFLQTLGYNARSINEGQAVVFSELQEENFTGIGISFGGGMCNVSVSFMSMPMITFSIP
                     KGGDYIDRNVTEVLGEGNLTKVRLEKEENLDLSRQPKDELSRALHIYYEDVMQSLIDR
                     LRAEFRASSQLPKIDRPMSIVLAGGTAKPAGFLQKFEGMLRAGGEFPIEVADVRMAKD
                     PLTATAHGCYIAALSETK"
     gene            complement(1377..2954)
                     /locus_tag="VGB76_17475"
     CDS             complement(1377..2954)
                     /locus_tag="VGB76_17475"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEY0100741.1"
                     /translation="MFAETKPQTSSVSGLTTAEATLFYSKTVERYARFFDRPEQRLRF
                     LNNTIAHQLACKEKLDHALESHSFLKKFGLYDRIKNKGFYDRLLDLWLHRHICRELKD
                     LLPSASSERRQLLRHSKASFTTRLLFRCYEMRRLLYGAAVATCVVAILGVGYTAVWSA
                     RHATSFLASRRQVERKLANVAPSDQNAVVAGSSVKHLPDYRPEKVWLVEQRETYERYS
                     NGARIQTDYETENHTRGYYVLPHGQGALVKANAGSSVGEVRRAPVGIVYHTSESDMLP
                     FTSDNNASIEVRTRGLLEYVRKNKSYNYLIDRFGQVYRVVRDTDAAHHAGNSVWSDAE
                     GVYVGLNESFLGVCFETNSKSESLDEQLTEAQLVAGRLLTQVLRSRYNIDDADCVTHG
                     LVSVNPSNMLICYHHDWARNFPFEAFGLSDKYKVAPASVSEFGFTYDEEITEKLGGDV
                     WAGVKLAEEEFRRRAEGLNKTPEEMRRQMRERYQAEMNLTLRLRTTHPPTEAEASRQT
                     DTPATTPNASRPNARQT"
     gene            complement(3020..4192)
                     /locus_tag="VGB76_17480"
     CDS             complement(3020..4192)
                     /locus_tag="VGB76_17480"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY0100742.1"
                     /translation="MRIVCPKCELKGQVDVATASAKMRIACVRCATTFDAISADGQIQ
                     VLLPQASHVEMALERVGSDVPAIAPETASPVETQEESQLDFASQTESTPAPTDDSFAS
                     STDVSITDVSIEETLETSESAWAQETAKEPVWQSVGTQSQETPVVEQAVSFVEPPVKS
                     VGVNKAGQPSDAYGLGVRLMRVSPMCLLLAGLSFISFIVLCNWLIKPGEQTGDTARML
                     VATDNHATNQSPSRVVVPDSTTQSPVNSQPAQPATQSGVSFIPTEATEATASKATAQG
                     AETIVKPAAEEKPAVEEKKASPSSSTNAASEAQAGKLTIQIGSYNEAAQADERVANLK
                     SAGYEARSVAVEIPKRGTWYRVQSGRFVNRDEAERYGKQLREKGVVSSYITTDVQE"
     gene            4832..>6699
                     /locus_tag="VGB76_17485"
     CDS             4832..>6699
                     /locus_tag="VGB76_17485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0100743.1"
                     /translation="MIAANTQNLVRPLKWFGIALGGGALIISALRLSVGQIDVPFLLL
                     ALVAINIGTRITIKIPHVRGQVSLSDTFVFLSMLLFAGEATVILAAVAALCASLRFSK
                     QSSTLLFNSAIAAASTFLILSLLRYKFGAVNALPHGHYSSTLILAISLIALVQSLIHS
                     GIVALSEGEVNSATLWHRWGKVFVWTSLTYFTVAASAGVIAKLVGIAGFLPSIAATTL
                     LAIGHFVYGIYLKDIYTSVTQARRMERQVATLQESEELFRSAFDFAAIGMALVSTKGR
                     WLQVNRSLCEILGYAEEELLAINFQKVMHPDDLGTALSQIKQLLKGKIPSYQMEKRYL
                     HKQGHAVWVLWSVSRVRESHTGLVHLIFQIQDITDRKRAEERLLHDAFHDALTGLPNR
                     TLFIDHLKLAIARMQRNPEQIFAVLFLDLDRFKIINDSLGHMIGDQLLVGIARRLETC
                     LRPGDTVARVGGDEFTILLEDMQDETEAISIAERIQKEISVPFALSGREVFTTVSIGI
                     APSSIEYTQSDDILRDADTAMYRAKTLGKARHEVFDKAMHAVAMNLLQMETDLRRALE
                     RQEFFIQYQPIVALDNFNLRGFEALVRWQHPERGLISPMDFIPVAEDTGQILPIGQ"
BASE COUNT         1318 a         1919 c         1923 g         1539 t
ORIGIN      
        1 catcggttca ttgattcaat gaagaaatga atcaatgaat caattcctta aaaagttacg
       61 gccggcatgt gcgcgcacgc cggccgtaac gtgagcaggc catatggtaa ggggaaagga
      121 gaacacccct ttaccccatg cctgcaagct catggttgtg tctagtttat aaacccgttc
      181 gtgagtcgct tacttcgttt cggagagcgc ggcgatgtag cagccgtgcg ccgtcgcggt
      241 gagcgggtct ttcgccatgc gcacgtcggc gacctcgatg gggaactcgc cgcccgcgcg
      301 cagcatcccc tcgaacttct gtaggaagcc cgccggtttc gccgtgccgc ccgcgagaac
      361 gatagacatc gggcggtcaa tcttcggcag ttggctcgac gcacgaaact cggcgcgcag
      421 acggtcgatc aaactctgca tcacgtcttc gtagtagatg tgcagcgcgc ggctcagttc
      481 gtctttgggc tggcgcgaga ggtcgaggtt ctcttccttc tccagacgca ctttggtcag
      541 gttgccttcg cccaaaactt ccgtcacgtt gcggtcgatg tagtcgccgc ccttcgggat
      601 gctgaacgtg atcatcggca tggacatgaa cgagacggag acgttgcaca tcccgccgcc
      661 gaaactgatg ccgatgcccg tgaaattttc ttcctgcaac tcggagaaga cgactgcctg
      721 accttcgttg atcgagcgcg cgttgtagcc gagcgtttgc aggaaactgc gcagcaccgc
      781 ttcgtgatag acgagtttac cgttgacgcc ttcgcccttg cccggcacgc tgaaatagag
      841 catctctttg ccccgcccgc gcggcacgat gcgctggatg atgagttgca tgatgtgctg
      901 gctcatctct tcctgcgcgt tcaagaggcc gtcgcgcatc gggcggcgcg catccacgtt
      961 caggaagctg gcgaagaaat tcgagtcgtt gccatagacg tagaggttct tgcagttgcg
     1021 ctcgtagacc atgttcttgt gcttgagcac gttctcggcc atctccgaac tcggaacggc
     1081 gacgaaggcg ttcagttgcg agcgcgaatc cttcgcggag gaactatcgg ccatcacgat
     1141 gcggctcgtg ccgatgtcca gcccgattgc gcccgtcttg gcgttgacgg attcgttctt
     1201 gttaagttca ggcttattcg acgtaacttg attcttctct tccaacattt ccgtttctcc
     1261 ttttggtgat cgggacgcga aagaacgtcc tttgtggttt ccgttgtgaa cgagtcacaa
     1321 gcagtcgggc gcagatttaa aacagagcgt gtgtgattga gtgaccctgt ggtcgtttaa
     1381 gtttgccgcg cgttcgggcg cgacgcgttt ggcgtggtgg ccggggtgtc cgtctgacgg
     1441 ctggcctctg cctccgtcgg cgggtgcgtg gtgcggagcc tcaaggtgag gttcatctcc
     1501 gcctgatagc gttcgcgcat ctggcgacgc atctcttcgg gcgtcttgtt caagccttcg
     1561 gcgcggcgtc tgaactcttc ttcggcgagc ttcacgccag cccagacatc gccgccgagc
     1621 ttctccgtga tctcttcgtc gtaggtgaaa ccgaactcgc tgacgctcgc cggagcgacc
     1681 ttgtacttgt cagataaacc gaaggcttcg aaagggaagt tgcgcgccca atcgtggtga
     1741 tagcagatca gcatgttcga gggattgacg gagacgaggc cgtgcgtcac gcagtctgca
     1801 tcgtcgatgt tgtagcggct gcgcaagacc tgcgtgagca aacgacccgc gacgagttgc
     1861 gcttcggtca actgttcgtc gagcgactcg gatttgctgt tcgtctcaaa acagacgccg
     1921 agaaaacttt cgttgaggcc gacatagacg ccttccgcat ccgaccagac ggagttgcct
     1981 gcgtgatgcg ccgcgtccgt gtcgcgcacg acgcgataaa cctgcccgaa gcggtctatg
     2041 agatagttgt aggatttgtt cttcctgacg tattcgagca gtccgcgcgt gcgcacttcg
     2101 atggaggcgt tgttgtcgga cgtaaacggc agcatgtcgc tctcggaagt gtgatagacg
     2161 atgccgacgg gcgcgcggcg gacttcgcct acgctgctgc cggcattcgc cttgacgagt
     2221 gcgccctgac cgtgtggcag aacgtaatag ccgcgcgtgt ggttctccgt ctcgtagtcg
     2281 gtctggatac gcgcgccgtt gctgtagcgt tcgtaagtct cccgttgctc gaccagccaa
     2341 actttctccg gacggtaatc cggcagatgt ttgacgctcg acccggcgac gaccgcattt
     2401 tggtcgctcg gcgcaacgtt ggcgagcttg cgttcaacct gacgccgcga ggcgaggaaa
     2461 gaggtcgcgt ggcgcgcaga ccagacggcg gtatagccga cgccgaggat tgcgacgaca
     2521 cacgttgcga ccgccgcgcc gtagagcagg cggcgcatct cgtagcaacg aaaaagcagc
     2581 cgcgtggtga aactggcttt gctgtgccgc aagagttggc gtcgctcaga cgaagcggac
     2641 ggcaacagat ctttcaactc gcggcagatg tggcgatgaa gccagaggtc taagagacga
     2701 tcataaaaac ccttgttttt gatccggtca tagaggccga actttttgag aaagctatgg
     2761 ctttcgaggg cgtggtcgag cttctctttg caggcgagtt ggtgtgcgat ggtgttgttg
     2821 aggaaacgca ggcgttgttc aggacggtcg aaaaaacggg cgtaacgctc aaccgtcttc
     2881 gaatagaaga gcgtcgcttc cgctgtcgtc aatccactga ccgaagatgt ttggggctta
     2941 gtttcggcga acatcgctat agtttgctcg ttgaagccgg cgcggaaaat agttgctctc
     3001 gaaagtcgag cgtcattaac tactcttgaa cgtcggtcgt gatgtaactc gacacgacgc
     3061 ccttttcgcg tagttgcttg ccgtatcgct ccgcttcgtc acgattgacg aaacgcccgc
     3121 tctgcacccg ataccaggtg ccgcgcttcg gaatctccac cgccacgctg cgcgcctcgt
     3181 atccggcaga cttcaagtta gcgacgcgtt catcggcctg cgccgcttcg ttgtatgaac
     3241 cgatctggat cgtcaacttg cccgcctgcg cttcgctcgc cgcgtttgtc gaagacgaag
     3301 ggcttgcctt cttctcttca accgcaggtt tctcttcagc cgcaggcttc acgattgttt
     3361 ccgcgccctg cgccgtcgct ttggaagccg tcgcctctgt ggcttcggtc gggataaaag
     3421 agacgccgga ttgagtcgca ggttgtgcgg gttgactgtt gaccggagat tgagttgtcg
     3481 agtcaggcac gacgacgcgt gaaggcgact ggttggtggc gtgattgtcg gtcgcaacga
     3541 gcatgcgcgc cgtgtcgcct gtctgctcgc ccggcttgat cagccagttg cagagaacaa
     3601 taaacgagat gaaagaaagc cccgcaagca acagacacat cggcgagacg cgcatcagac
     3661 gcacgcccag accataagcg tccgaaggct gcccggcctt gttcacgccc acagatttga
     3721 ccggcggttc aacaaagctg accgcctgct cgacaacggg cgtctcctgc gattgtgtcc
     3781 ccacgctctg ccacacaggc tcttttgcag tctcctgcgc ccatgcggac tcagacgttt
     3841 ccaacgtctc ttctatcgac acatcagtta tcgacacatc cgtcgaggac gcgaaagagt
     3901 cgtctgtcgg ggcaggcgtg ctttcagttt gtgaggcgaa gtcgagttgt gattcttctt
     3961 gtgtctcgac gggcgatgct gtctccggcg cgatagcggg cacgtcgctg ccgactcttt
     4021 cgagagccat ctcaacgtgg ctagcctgcg gcagcaggac ttgaatctga ccatcggctg
     4081 aaatggcatc aaaggtcgtg gcgcaacgga cgcaggcaat gcgcatcttc gcgctggcgg
     4141 tcgccacatc cacctgtccc ttcaactcgc acttcggaca aacaatcctc atagactcac
     4201 tcccaattca gccatctcaa atttcgagca acgcctgcgc cttgcgcttc ctgtcggtca
     4261 ccccaagttt tatcggaaat ttatctggct cacgctgctg gcaactcaaa ctcgaaaggg
     4321 tcaaaggttc gcaggcggtt tgtaagcaaa aatcttgttg acaagagcaa cggcggcgtc
     4381 cgtcgcatcg ggcgggcggt ttctaaacgc gcggaaaagc cttcaatccg cacaaaaaga
     4441 ttctaaatca tcaacaatgt taaagcaagt aaaaactaaa attgcacctt ttgtcgttaa
     4501 ggttggttaa attattgata tttatgggtt tgcggggcag gcggactccg ccgaaggaca
     4561 agctgcgcta tttaatttgt agattttagc tgttcgaagg gagaaattcg gtctaagtga
     4621 cgactgacca tgccagcttg accgtgcagt ttgaccggag agggagggtt tttacggttg
     4681 gtgaggggga ttttctagag aaacggggga cttttattga gaggggaagt tggcataagg
     4741 tttgtacgcg agttggctgc cttccgtctg gtaggacagg cgagcgcgtt tccaaatcca
     4801 cttttagtag caccctcgat ttcctgtcat catgatcgct gcaaacacac agaatcttgt
     4861 ccgtccactc aagtggttcg gcatcgcttt gggtggcggt gctttaatca tctcggcgct
     4921 ccggctctcc gtgggacaaa ttgatgtccc atttttgctg ttggctctcg tcgcgatcaa
     4981 catcgggacg cgcatcacga tcaaaatccc acacgtacgc gggcaggttt cactctcgga
     5041 cacgttcgtc tttctgtcga tgttgctctt cgcaggtgaa gcgacggtga ttctcgccgc
     5101 cgtcgcggca ctgtgcgcgt ctctacgctt tagcaaacaa agctcgactc tcctcttcaa
     5161 ctccgccatc gccgccgctt ccaccttcct catcctctca ctcttgcgtt acaagttcgg
     5221 agccgtcaac gctctgcctc acggtcatta ctcctcaacg ctcatcctcg ccatcagcct
     5281 catcgcgctc gtccaatctc tcattcactc cggcatcgtc gccttgagcg agggggaagt
     5341 taactctgct acgctctggc acaggtgggg gaaagttttt gtctggacaa gcctgaccta
     5401 cttcaccgtc gcggcctcgg cgggcgtcat cgccaaactc gtcggcatcg cgggcttcct
     5461 tccttcaatc gccgcgacca cgctcctcgc catcggccac ttcgtctatg gcatctattt
     5521 gaaagacatc tacacgtcgg tcacgcaggc gcgacggatg gagcggcaag ttgcgacctt
     5581 gcaggagagc gaagaactct tccgcagcgc gtttgacttc gcggccatcg gcatggcgct
     5641 cgtctctacg aagggacgtt ggctgcaagt gaaccgctcg ctctgtgaaa tcctcggcta
     5701 cgcggaggaa gaactgctgg cgatcaactt ccagaaagtc atgcacccgg acgatctggg
     5761 cacagccttg agccagatca agcagttgct caaaggtaaa attccttcct accagatgga
     5821 gaagcgttac ctgcataagc aggggcatgc cgtctgggta ttgtggagcg tctcacgcgt
     5881 ccgcgagtcg cacacgggtc tggtgcatct catcttccag attcaggaca tcaccgaccg
     5941 caagcgcgcc gaggaacgct tgctccacga cgccttccat gacgcgctga ccgggctacc
     6001 caaccgcacg ctcttcatcg atcacctcaa actcgctatc gcgcgcatgc agcgcaaccc
     6061 cgaacaaatt ttcgcggtgc tctttttaga cctcgaccgt ttcaagatca tcaacgactc
     6121 actcgggcac atgatcggcg atcaactcct cgtcggcatc gcgcgcaggt tggagacttg
     6181 cctgcgtccg ggcgatacgg tcgcgcgtgt gggcggcgac gagttcacga ttctgttgga
     6241 agatatgcag gacgagacgg aagctatctc gatcgccgag cgtattcaaa aagagatttc
     6301 cgtgcccttc gccctgagcg ggcgcgaagt gtttaccacc gtcagcatcg gcatcgcgcc
     6361 gagttcgatt gaatacacgc agtcggatga tattctgcgt gatgcggaca cggcgatgta
     6421 tcgcgcgaag acgctcggca aggcgcgcca tgaagttttc gataaggcga tgcacgccgt
     6481 ggcgatgaac ctcttgcaga tggagacgga cttgcgccgc gcacttgaac gtcaggagtt
     6541 cttcattcaa tatcaaccca tcgtcgcgct cgacaatttc aacttgcgcg gctttgaagc
     6601 gctcgtgcgc tggcagcacc cggagcgcgg cctgatctcg ccgatggatt ttattcccgt
     6661 cgcggaggac acggggcaga ttcttcccat cgggcagtg
//
LOCUS       DASWNS010000507         7601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_149360, whole genome shotgun sequence.
ACCESSION   DASWNS010000507 DASWNS010000000
VERSION     DASWNS010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149360"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VGB76_17490"
     CDS             <1..377
                     /locus_tag="VGB76_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100744.1"
                     /translation="YFQIPQQAFGERIFHFTQATFEPYLQSDFRVTVGPYKVVNLTLV
                     KVEDLRPRPRKGMARTEGECYTLLFKAPDKLSDLQQTYVLQHEALGKFSLFLVEAGEK
                     ESDVYYLATINRMRISDTPTKL"
     gene            531..1058
                     /locus_tag="VGB76_17495"
     CDS             531..1058
                     /locus_tag="VGB76_17495"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0100745.1"
                     /translation="MERPLDESGQVVLRAVQPEDEDFLLQVYAASRADEMALVDWDEA
                     QKMAFLRSQFAAQAAQYHARFPDAEYSIILYKERPVGRFWIGRTPEQIRLLDIAILPE
                     FQNRGVGAVLLKSLLAESEATAKPLRHMVFKMNTAALRFYERFGFSPIEDVGAYIHME
                     RRPAGVRPAPATARD"
     gene            complement(1042..1341)
                     /locus_tag="VGB76_17500"
     CDS             complement(1042..1341)
                     /locus_tag="VGB76_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100746.1"
                     /translation="MLEQLTVASLSEQLNTKFRLSVEPETFVELELVKVETHDDVPGQ
                     TERFSALFRGSLNQFLPQSTYAFEHEALGSFELFIVPLRQDGEGFYYEAVFNRVQ"
     gene            complement(1382..1454)
                     /locus_tag="VGB76_17505"
     tRNA            complement(1382..1454)
                     /locus_tag="VGB76_17505"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Val,seq:cac)
     gene            complement(1602..2090)
                     /locus_tag="VGB76_17510"
     CDS             complement(1602..2090)
                     /locus_tag="VGB76_17510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0100747.1"
                     /translation="MHKDLKRRKNERGFSLIELMIVIAIIGILVGVGIPAWQSSVRSA
                     NQAAAIRTLQAIAVEQRIYYNSHNRSSYGTFDQLIADGQLDKRYAGEEPLVEGYIFRL
                     KTTPKTGNQPPMYTVNADPQKPDGVTATGKEFYYIDGNASTIHVNGTQPAGPNDPPMG
                     GQ"
     gene            2422..3414
                     /locus_tag="VGB76_17515"
     CDS             2422..3414
                     /locus_tag="VGB76_17515"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0100748.1"
                     /translation="MRRLQTAHESEDGVDATVAAAGSVAQLHAAIESVIKGKSEAVRL
                     TLVGLLAGGHLLVEDVPGVGKTTLAQALARALDCEFQRIQFTSDLLPSDVIGLSVYNE
                     RAGAFEFKPGPIFANVVLADEINRTTPKTQSALLEAMGEGHVTVEGTTYKLPAPFIVV
                     ATQNPIEHHGTYPLPESQLDRFMLRLRIGYPDAEMEKVILRDRETRDPLAEMRAVLSA
                     ADIAALQRHVSGVKMDDALVDYLMRIVSATRNSEMLDLGVSPRGTLALFRAAQALALT
                     EDRAYCVPDDIKRLVLPVFAHRLVVNSRFSSSLRRSEEAEAVLREIMKTISVPL"
     gene            3452..4858
                     /locus_tag="VGB76_17520"
     CDS             3452..4858
                     /locus_tag="VGB76_17520"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0100749.1"
                     /translation="MRLSGLKHLRAWRNAILGTLLVAGGVVVAAVTSWARRSGDWELA
                     RIGAITSLVFVALITIFVVPPLLRSARNEVGRLDPPFQVTTGGVIFIGIFLSVAFAAY
                     NTGNNLLFLIFSVLASALFVAWSAARASLRDLVVSARFPDHIFAGEPAPVIVSLQNTK
                     RLLPSFSILIESRNQIFAEATEARGQRRKTLRRRGRRERPRYLRRALAYFIYVPHRAR
                     AEQRVEQLFPKRGHVLVTGFELSTRFPFGFFRLRRRLRSRNVELVIYPKLEAASDELH
                     LLPMNAGRMLSARRGAGHDLHSLRDYQPQDDMRHIDWKATARASRLIVREFASEDERR
                     VHLVFDTRAHETSASVGDAARRDFEARFERGVTVAASLVAHFLEERAEVRLTLGEEAG
                     HYGAGREHLYASLRRLALIRPQETRQDATHDNGHDLWSSIAATSHNADSHYVILLTTA
                     PPGSIPANVWRMSHVIYL"
     gene            complement(4973..6568)
                     /gene="hemG"
                     /locus_tag="VGB76_17525"
     CDS             complement(4973..6568)
                     /gene="hemG"
                     /locus_tag="VGB76_17525"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEY0100750.1"
                     /translation="MSRSFAASSKPRRRIVIVGGGISGLAAAHKLSQQARSDDPPLEV
                     LLLEASPRLGGTIRTYEREGFLLEGGPDAFISEKPEALELSKQLDLESHLLETGAAHR
                     RSFIVRGGRLRPVPEGFQLLAPSRFWPFVTTDVFSWAGKARMALELLLPRRALVAPEV
                     DDESLAEFVRRRFGREALERMAQPMVGGIYTADPEKLSLRATMPRFLEMERRHRSVIR
                     AMWRARRNSTTDTETRSTSGARYSLFLSFDGGMQMLPDRLAAALPQGTTRLGARVEAI
                     EFDAQAGSWKLRLSANRTTRGTETLSRAEAKSLNEPASENETTRAGETNRAGGLSAAG
                     ELIEADGVCLALPAYASAQLLGAVDTSLAAELAAIPYASTATVNLAFRRADIPHPLDG
                     FGFVVPYIERRRVIACTFSSVKFAGRAPAGHALLRAFVGGALQPEMYALDEEQMIAAV
                     CADLRALLGIERAPLFAHVEKWPRSMAQYHLGHLARVARIREHLRRFPTLQLAGNAYG
                     GAGIPDCIRSGHAAADALMNAER"
     gene            complement(6681..7289)
                     /locus_tag="VGB76_17530"
     CDS             complement(6681..7289)
                     /locus_tag="VGB76_17530"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY0100751.1"
                     /translation="MRDLQHALARIPYERRRNAHVAFTAHSIPLSMATNCDYQAQLLE
                     TCRLVAQGAGHARWQLVFQSRSGSPMQPWLEPDICDHLRELRRAGATDVVVAPIGFIS
                     DHMEVLYDLDTEAQQVAHEIELNLIRAVTVGTHPAFIKMIRELILERMTSDPVRRTLG
                     ARGAKEDVCPADCCLSGAGRPGAVHPVNSEHAAPAHERPPAN"
     gene            7272..>7601
                     /locus_tag="VGB76_17535"
     CDS             7272..>7601
                     /locus_tag="VGB76_17535"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY0100752.1"
                     /translation="MLQVAHHYELFGGVSPINGQNRALIAALERELRESDLKLPVYWG
                     NRNWHPLLADTLRQMRQDGRRRALAFVTSAYSSYSGCRQYREEIERAREAAGEGAPQV
                     DKLRVFYN"
BASE COUNT         1406 a         2408 c         2323 g         1464 t
ORIGIN      
        1 gctatttcca aattccccag caagccttcg gcgaacgcat tttccacttc acacaggcga
       61 cctttgaacc gtatttgcag agcgacttcc gcgtcacagt cggaccctac aaggttgtga
      121 atctgacgct ggtgaaagtc gaagacctca ggccgcgccc gcgcaaaggc atggcacgca
      181 ccgagggcga atgctacacg ctcctcttca aagccccgga caaattatcc gacctccagc
      241 agacctacgt gctccagcac gaagccctcg gaaagttttc tctgtttttg gtggaagcgg
      301 gcgagaaaga gagtgacgtt tattacctcg ccaccatcaa ccgcatgcga atatccgaca
      361 ctcctacgaa actgtaggcg cgaaccgccg acgccaacct tcgtgtcgca ctcttaagca
      421 gcctgtgcgc gcgcatctcc gacgctcacg cgcgcctgtt tccgcaggac gcccggcgtg
      481 tctataataa atttcctgaa cgggatattt tgagggagga gagaaagagc ttggagcgtc
      541 cacttgatga aagcggtcag gtcgtcttgc gtgcagtgca accggaggac gaagattttc
      601 tgctacaggt ctatgccgct tcgcgcgcag atgagatggc gctcgtggat tgggacgagg
      661 cgcagaaaat ggcctttctc cgctcgcaat ttgcagcgca agccgcgcag tatcacgcgc
      721 gctttcccga tgcggagtac agcatcattc tctacaagga acgtccggtg gggaggttct
      781 ggatcggcag gacgccggag cagattcgcc tgctcgacat cgcgattttg ccggagtttc
      841 agaatcgagg cgtcggcgcg gtcttgttga aaagtttact ggccgaatcc gaagcgactg
      901 cgaagcccct gcggcacatg gtctttaaga tgaatacggc ggcgctgcgt ttctacgagc
      961 ggttcggctt ctcgccgatt gaagacgtgg gcgcgtacat tcacatggaa cggcgacctg
     1021 ccggtgtccg gcctgcgccc gctactgcac gcgattgaag accgcctcgt aataaaagcc
     1081 ttctccgtcc tgccgcaacg gaacgatgaa caactcgaaa ctccccaatg cttcatgttc
     1141 gaacgcgtag gtgctttgcg gaaggaattg gttgagagaa ccgcgaaaga gcgccgagaa
     1201 tcgttccgtc tgccccggca catcgtcgtg cgtctctact ttgaccaact ccaactcgac
     1261 aaacgtttcc ggctccacgc tgagccggaa cttcgtgttc aactgctccg agagcgaagc
     1321 taccgtcagc tgctcaagca tcgtttctgt cctttatcat tgaatgggga aggaaatttg
     1381 atgctcgctg caggactcga acctgcgacc ccacgcgtgt gaagcgtgtg ctctaccaac
     1441 tgagctaagc gagcacgaga gaatgtctga aagaggcggc atgctaacaa agcgagcggg
     1501 gaagcgtcaa gcgaggtgcg cctgctgctt ccccggagtc gcgcggcggc gacgggaaat
     1561 ttctcgccgc cccgaacaat aactactgcc agtaactact actattgccc gcccatcggc
     1621 ggatcgttcg gaccggcggg ttgcgtgccg ttgacgtgga tggtgctcgc gttgccgtca
     1681 atgtagtaga actctttgcc ggtggcggtc acgccgtcgg gcttttgcgg gtcggcgttg
     1741 acggtgtaca tcggcggctg gttgccggtc ttgggcgtcg tcttcaggcg aaaaatatag
     1801 ccctcgacga gcggttcttc gcccgcgtaa cgtttatcca actgcccgtc ggcgatcaat
     1861 tgatcgaacg ttccgtaaga actgcgattg tgcgagttgt aataaattcg ctgctcgacg
     1921 gcgatggctt gcagcgtgcg gatggccgcc gcctggttgg ccgagcgcac gctcgactgc
     1981 cacgccggaa tcccgacgcc gacgagaatc ccgataatag cgatcacgat catcaactcg
     2041 atgagcgaaa atccgcgttc attcttgcga cgttttaaat ctttgtgcat tttcacttcc
     2101 tctaatatgg tgggaattta actccgccgt tctttacacg aaagagccgg gcgcaaagtt
     2161 cctttattcg cgcgctcgcg ggggcgaacc tccttcgact cgacggcgac gccgatttta
     2221 ctacaccgcc gcgcggcaag tcacatgcat agagcgactg gagttagatg caggatgagg
     2281 gcgttgagag gctagtgaaa cggaagtcga aagtcgaaaa acggggcgag cgaaactctt
     2341 taaccaacct ctgacctcta acctcccgtc tctctttcat gtagaatgct tgcacgcagc
     2401 caacacacag gggagtgatt gatgagacgt ttacagacgg cgcacgagag cgaggacggg
     2461 gtggacgcga cggtggccgc cgccgggagc gtcgcgcagc ttcatgcggc catcgagagc
     2521 gtcatcaagg gcaagagcga agccgtgcgg ctcacgctcg tcgggctgct ggcgggcggg
     2581 cacctgctcg tcgaagacgt gcccggcgtc ggcaagacca cgctcgcgca ggcgctcgca
     2641 cgcgcgctcg attgcgagtt ccaacgcatc cagttcactt ccgacctgct gccctccgac
     2701 gtgatcggcc tctcggttta taacgagcgg gcgggcgcgt ttgagttcaa accgggaccc
     2761 atcttcgcca acgtcgtgct ggcagacgag atcaaccgca ccacgccgaa gacgcaatcc
     2821 gccctgctcg aagcgatggg cgaaggccac gtcaccgtcg agggcacaac gtataaactc
     2881 cctgcgccct tcatcgtcgt cgccacgcag aacccgattg agcatcacgg cacctacccg
     2941 ctgcccgaat cgcaactcga ccgcttcatg ctgcgcctgc gcatcggcta ccccgacgcc
     3001 gagatggaaa aagttatcct gcgtgaccgc gagacgcgcg acccgctcgc ggagatgcgc
     3061 gccgtgctct ctgccgccga catcgccgcc ctgcaacgcc acgtctccgg cgtcaagatg
     3121 gacgacgcgc tggtggatta cctgatgcgc atcgtctcgg ccacgcgcaa ctccgagatg
     3181 ctcgacctcg gcgtctcccc gcgcggcacg ctcgcgctct ttcgcgccgc gcaggctctc
     3241 gccctcacgg aagaccgcgc ctactgtgtg cccgacgaca tcaaacggct cgtcctgccc
     3301 gtcttcgcgc accgcctcgt cgtcaactcg cgtttctcgt cctccctgcg ccgcagcgaa
     3361 gaggccgagg ccgtgctccg cgagatcatg aagacgatca gcgtgccgct ctgagactca
     3421 aactcgcgag gcgttaccgt cattgaattc gatgcgactt tccgggttga aacatttgcg
     3481 tgcgtggcgc aacgcgatac tggggacgct gctcgttgcg ggcggagtcg tcgtcgcggc
     3541 ggttacgtcc tgggcgcgac gcagcggcga ttgggagttg gcgcgcatcg gtgcgatcac
     3601 ctcgctcgtg ttcgtcgctt tgatcacgat cttcgtcgtg ccgccgctgt tgcgttcggc
     3661 gcgcaacgaa gtgggacgac tcgatccacc ctttcaagtg acgacgggcg gcgtcatctt
     3721 catcggcatc ttcctctcgg tcgccttcgc cgcctacaac acgggcaaca atctgctctt
     3781 cctgatcttc tcggtgctgg cctcggcgct cttcgtcgcg tggtcggcgg cgcgcgcctc
     3841 cctgcgcgat ctcgtcgtct cggcgcgttt cccggaccat atcttcgcgg gcgaacccgc
     3901 gcccgtcatc gtcagtctac agaacacgaa gcgtctgctg ccctccttct cgatcctgat
     3961 cgaatcgcgc aaccagattt tcgcggaagc tacggaagcg cgcggccagc gcaggaagac
     4021 gctgcgacga cgcggacgac gcgagcgtcc gcgctacctg cggcgcgcgc tcgcctattt
     4081 catctacgtg ccgcaccgcg cgcgtgcaga gcagcgcgtc gaacagttgt tcccgaagcg
     4141 cggccacgtg ctcgtcacag gcttcgaact ctccacgcgt ttccccttcg gcttctttcg
     4201 cctgcgccga cgcctgcgct cgcgcaacgt cgaactcgtc atctacccga aactcgaagc
     4261 cgcgagcgac gagttgcacc tgctgccgat gaacgccggg cgcatgctct cggcgcggcg
     4321 cggcgcgggg cacgacctgc acagcctgcg cgactatcaa ccgcaggacg acatgcgcca
     4381 catcgattgg aaagcgacgg cgcgtgcgag tcggctcatc gtgcgcgagt tcgcgtcgga
     4441 ggacgagcgg cgggtgcatc tcgtcttcga cacgcgcgcg catgaaacga gcgcgtccgt
     4501 gggcgacgcg gcgagaagag atttcgaggc gcgtttcgaa cggggcgtga cggtcgcggc
     4561 ttcgctcgtc gcacactttc tcgaagagcg cgccgaagtc cgtctcacgc tcggcgaaga
     4621 agcggggcac tacggcgcgg gacgcgaaca tctctacgcc tccctgcgcc gcctcgcact
     4681 catccgcccg caggagacga ggcaagacgc gacgcacgac aacggccacg atctctggtc
     4741 gtccatcgcc gccacctccc acaacgccga ctctcactac gtcatcctgc tcaccaccgc
     4801 cccgcccggt tccatccccg ccaacgtctg gcgcatgtcg cacgtcatct atctctgaaa
     4861 ccatgaacca gaactcagaa gtcagaaaac agaattcagg agtcagaagc ataagacagc
     4921 gcatgttttc ttctgacttc tgtattctga ctcctgaatt ctgttttcta gttcagcgtt
     4981 ccgcgttcat caatgcgtcc gccgccgcgt ggccgctgcg gatgcagtca gggatgcctg
     5041 cgcctccgta ggcgtttccg gcgagttgga gagtggggaa gcgtcgcaag tgttcgcgga
     5101 tgcgcgcgac gcgtgcgagg tggccgagat gatattgagc catcgagcgc ggccattttt
     5161 cgacgtgcgc gaacaggggc gcgcgttcga ttccgagaag ggcgcgcagg tctgcgcaga
     5221 ccgctgcgat catctgttct tcgtcgagcg cgtacatctc cggctggagc gcgccgccga
     5281 cgaaagcgcg cagcaacgca tggcctgcgg gcgcgcgccc ggcaaacttc acgctgctga
     5341 aggtgcaggc gataactctg cgccgttcga tgtagggaac gacgaagccg aagccgtcga
     5401 gcgggtgcgg aatgtcggcg cggcggaagg cgagattgac ggtggcggtc gaagcataag
     5461 gaatcgccgc gagttcggcg gcgaggctcg tgtccactgc gccgagcagt tgcgccgacg
     5521 cgtaagcggg caacgcgagg cacacgccgt ccgcctcgat caattcaccc gccgcgctca
     5581 agccgccagc acgattcgtt tcgcccgcgc gggtcgtttc gttttcgctc gccggttcgt
     5641 taaggctctt tgcctcggcg cggctcaatg tctccgtccc gcgagttgtc ctgttcgcgc
     5701 tgaggcgcaa cttccaactc cctgcctgcg cgtcgaactc aatcgcttcg actctcgcgc
     5761 cgagtcgcgt tgtgccctgc ggaagcgcgg cagcgaggcg gtcgggcagc atttgcatgc
     5821 cgccgtcgaa ggagaggaag agactgtagc gtgcgccgct cgtcgagcgc gtctccgtgt
     5881 ccgtcgtgct gttgcggcgc gctcgccaca tggcgcggat gacgctgcga tgcctgcgtt
     5941 ccatttcgag aaagcgcggc atcgtcgcgc gcaggctcaa tttctccggg tctgccgtgt
     6001 aaatgccgcc gaccattggt tgcgccatgc gttccagtgc ttcgcgcccg aaacgccgcc
     6061 gcacaaattc cgcgaggctt tcgtcgtcaa cttccggcgc gacgagggcg cgacgcggca
     6121 gcaacagttc gagcgccatg cgcgctttgc ccgcccaact gaaaacgtcc gtcgtgacaa
     6181 aaggccagaa gcgcgagggc gcgagcagtt gaaaaccttc gggcacggga cgcagtcgtc
     6241 cgccgcgcac gatgaaggag cgacgatgcg ccgcgcctgt ctcaagtaga tgagattcga
     6301 gatcgagttg cttggagagt tcgagggctt cgggcttttc ggagatgaaa gcgtccggac
     6361 cgccttcgag cagaaagcct tcgcgttcgt aggtgcgaat cgtgccgccg agtcgtggcg
     6421 aggcttcgag caggagcact tcgagcggcg ggtcgtctga gcgcgcctgt tgtgacagct
     6481 tgtgcgccgc cgccagcccg ctgatgccgc cgccgacgat gacgatgcga cggcgcggct
     6541 tagagcttgc tgcaaaacta cgactcatca cttaaccctt tgcgccttcc gttttaactc
     6601 tgcgcccttg cgggaaaatt attctcacgc agaggcgcaa agacgcaaag ggagacaggc
     6661 agtggttttg tcgcgcacgc ttagttcgcc ggggggcgtt catgcgccgg ggcggcgtgt
     6721 tcggagttaa cggggtggac tgcgccgggg cgtcccgctc ccgacaggca acagtctgcg
     6781 gggcagacat cttcctttgc gccgcgcgcg ccgagggtgc gccgcacggg gtcgctcgtc
     6841 atgcgttcga ggatcaattc acggatcatc ttgatgaacg cgggatgcgt gccgacggtg
     6901 accgcgcgga tcaggttgag ttcgatctcg tgtgcgacct gctgcgcctc cgtgtcgagg
     6961 tcgtataaca cttccatgtg atcggagatg aagccgatgg gggcgacgac cacatcggtc
     7021 gcgcccgcgc gtctcaactc gcgcaggtga tcgcagatgt ccggctcaag ccacggctgc
     7081 atgggcgaac cgctccggct ttgaaagacg agttgccagc gcgcgtgacc cgcgccctgc
     7141 gcaacgagac gacaggtttc cagcagttgc gcttgatagt cgcagttcgt cgccatcgag
     7201 agcgggatgc tgtgcgcggt gaaggcgacg tgcgcgttgc gccgccgctc gtaaggtatc
     7261 cgcgcgagcg catgttgcag gtcgcgcatc actacgaact gttcggcggc gtctcgccca
     7321 tcaacggtca gaaccgcgcc ctcatcgccg ccctcgaacg agaactccgc gagagcgatc
     7381 tcaaacttcc cgtctactgg ggcaaccgca actggcatcc gctcctcgcc gacaccctcc
     7441 gccagatgag acaggacggc agacgccgcg cgctcgcctt cgtcacctcc gcttacagtt
     7501 cctattccgg ctgccgacag tatcgagaag agatcgagcg tgcgcgcgag gctgcgggcg
     7561 agggcgcgcc gcaggttgat aagctgcgcg tcttctacaa c
//
LOCUS       DASWNS010000508         4998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_96786, whole genome shotgun sequence.
ACCESSION   DASWNS010000508 DASWNS010000000
VERSION     DASWNS010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96786"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VGB76_17540"
     CDS             <1..394
                     /locus_tag="VGB76_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100753.1"
                     /translation="AMLNYIEETPEHEGIIQAIRTHLRDTTRCATTTGYGPRFLHSTG
                     QLHKGGSASGVFLQLTAPDVKDLPIPGQPYTFSTLKEAQALGDFRSLSTRGRRAIRID
                     LGADVPAGLERLQTLIGEALPRSGATGA"
     gene            515..1966
                     /gene="gndA"
                     /locus_tag="VGB76_17545"
     CDS             515..1966
                     /gene="gndA"
                     /locus_tag="VGB76_17545"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEY0100754.1"
                     /translation="MSTPEESSKTAKIGMIGLGVMGENLARNIERNGYSIAVWNREPK
                     KVDEFVGRLEGHQVIGAHTPEEFVKSLERPRKAILLVKAGDPVDWTVNQIKPFLEEGD
                     IVIDGGNSYFMDTERREQELKAEGFNFIGSGVSGGEKGALLGPSLMPGGDRAAYEQIR
                     PIWEAIAAKVDDGPCVTYIGPGGAGHFVKMVHNGIEYGDMQLIAEAYDMMRSILGMEA
                     TEMADVFDEWNRGDLESFLIEFTAQILRVKDPETGKPLVDLVLDKAGQKGTGKWTSDI
                     ALDLGIVTPTIDAAVDTRNLSSRKEERVEASKQIKGPERAAFTGDKREMIQAIHDALY
                     ASKICSYAQGMNLIRAGSETYKWDIDLGECARIWKGGCIIRAQFLGKIKQAYQRRADL
                     PNLLLDPDFNAFVEQAQPNWRRAVATAIQTGVAVPGMSASIGYFDTYRTANLPLNLTQ
                     AQRDFFGSHTYERTDKPGQPAVHTEWEEMLKKG"
     gene            2074..3618
                     /gene="zwf"
                     /locus_tag="VGB76_17550"
     CDS             2074..3618
                     /gene="zwf"
                     /locus_tag="VGB76_17550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY0100755.1"
                     /translation="MSATLLENPLRAGVRLERTAEPCIVVIFGATGDLTKRKLVPALY
                     RLVQERLVPAEFAIVGAARTEMTSEEFRANMREAVVQFSEAKRVDEEVWESFAQGLHY
                     LRVDIGASADYVTLGAKLDEIDAARGTQGNRVFYLSTAPSLYAEAVRQLGQSGLAKPK
                     EKGWVRVIIEKPFGTDLASAQALNRDIHQHLDESQIYRIDHYLGKETVQNLLVFRFAN
                     GIYEPLWNRQYIDHVQLTNAETVGVEGRGGYYEKAGVVRDMLQNHVFQVLSLVAMEPP
                     ASLNSESVRDEKIKAMTSVRPFTPERIRAECVRGQYGAGSINGKPVPGYREEEGVAPD
                     SATETFAMLTMYFDNWRWSGVPFYIRSGKRLAKRVTEIAIQFKDAPHKLFGSEMMEQM
                     TPNQLIIRVQPDEGITMRVAAKVPGQVTRIRDVNMDFRYGSSFGVQLAEAYERLLLDC
                     ILGDPTLYARTDMTERGWDIVMPILDEWAATKADAPFPNYEAGTWGPQATHELLEKEG
                     RRWRRP"
     gene            3805..4962
                     /locus_tag="VGB76_17555"
     CDS             3805..4962
                     /locus_tag="VGB76_17555"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEY0100756.1"
                     /translation="MSREIPQSTAAGDVAQGIDVVRLEKELAASWQNEAGAGESGVTR
                     VCVLNLIVYATPADDRAKIDAVLNEVTAHTPSRALVLIADRVSTEARLEASVSALCQQ
                     ESRGGAKRVCGEQVVIEASGAQIETTASAIEPLVVPDVPVFLWWKDIPHEEDKLFNRL
                     VELSDRVVIDSLVFDEPHEDLKRLAQLIRERRQYLLLSDVNWGRLTSWRNLIAGFWDV
                     ADYRAHLDALDHVLIEFDPPDNSSGEIAAQALLVLGWLASRLKWQATGEFKREGQSAV
                     WMLTAGERKLRVELRANDDRHGCDSLIAGVTLKSATSGAEFFVGVNEEWTKLETSAKI
                     GDAHTVGRVVTYEAKSEGERLSRELGMLARDVIYEQAVASVGQLLEGLQKS"
BASE COUNT         1079 a         1479 c         1507 g          933 t
ORIGIN      
        1 cgccatgctc aactatatcg aagagacgcc cgaacacgag ggcatcattc aagcgattcg
       61 cacgcacctc cgcgatacga cgcgctgcgc cacgacgacc ggctatggac cccgcttcct
      121 gcattcgacg ggacagttgc acaaaggcgg ctcggcgtcc ggcgtcttcc tgcaactcac
      181 cgcgccggac gttaaagatt taccgattcc ggggcagcca tacactttca gcaccttgaa
      241 agaggcgcag gcgctcggcg attttcgctc gctctcgacg cgcggacgcc gcgccattcg
      301 catcgatctc ggcgcagacg tgcccgccgg gctggagcgt ctacagacgt tgatcggcga
      361 agcccttcca cgctccggcg cgacgggagc ctgatagaaa ggatgaggga tgaggggatg
      421 tgggatgaat cagagaagca cacctgctct tgactcattc tgattcatcc cacatcccac
      481 atcccgcatc cctttaatat agtgaggaaa tcttatgtca acacctgaag agagtagtaa
      541 gaccgcgaag atcggcatga tcggtctggg cgtgatgggc gagaatctgg cgcgcaacat
      601 cgagcgcaac ggttattcca tcgcggtgtg gaatcgcgag ccgaagaaag ttgacgagtt
      661 tgtcgggcga ctggaaggcc atcaagtgat cggcgcgcat accccggaag aatttgttaa
      721 atcgctcgaa cgcccacgca aagcgatcct gctcgtcaaa gcgggcgacc ccgttgactg
      781 gaccgtcaac cagatcaagc cgtttctgga agagggcgac atcgtcattg atggcggcaa
      841 ctcttacttc atggacaccg aacgccgcga gcaggagttg aaagccgaag gcttcaactt
      901 catcggctcc ggcgtctccg gcggcgagaa aggcgcgctg ctcggcccct cgctcatgcc
      961 cggcggcgac cgcgcagcct acgagcagat tcgcccgatc tgggaagcga ttgcggcgaa
     1021 agtggatgat ggtccctgcg tcacttacat cggacccggc ggcgcgggtc acttcgtcaa
     1081 gatggtgcac aacggcatcg agtatggcga catgcaactg atcgccgaag cctacgacat
     1141 gatgcgcagc atcctcggca tggaggccac ggagatggcc gacgtgttcg acgagtggaa
     1201 ccggggcgac ctcgaatctt tcctcatcga gttcaccgcg caaattctgc gcgtcaaaga
     1261 cccggagacg ggcaagccgc tcgttgatct cgtgctcgac aaggccgggc agaaggggac
     1321 gggcaagtgg acttccgaca tcgcgctcga cctcggcatc gtcaccccga cgattgatgc
     1381 cgccgtagac acgcgcaatc tgtcttcgcg caaagaggag cgcgtggaag ccagcaagca
     1441 gatcaaagga ccagagcgcg ccgcgttcac gggcgataag cgcgagatga ttcaggccat
     1501 tcacgacgcg ctctacgcct cgaagatctg ctcctacgcg cagggcatga acctcatccg
     1561 cgccggttcc gagacttaca agtgggacat agacctcggc gagtgtgcac gcatctggaa
     1621 aggcggctgc atcatccgcg cgcagttcct cggcaagatc aagcaagcct atcaacggcg
     1681 cgcagacctc ccgaaccttc tgctcgaccc tgacttcaac gcgtttgtcg agcaggcgca
     1741 gcccaactgg cgtcgcgccg tggcgactgc gattcaaacc ggcgtcgccg tgcccggcat
     1801 gtcagccagc atcggctact tcgacaccta tcgcaccgcc aatctgcctc tgaacttaac
     1861 gcaggcgcag cgcgacttct tcggctcgca cacctacgag cgcacagaca aacccggcca
     1921 acccgccgtc cacaccgaat gggaagagat gcttaagaaa ggatgacggg aaaggatgag
     1981 gaatgagggg tgagggatga atcagaaaac cgttcacacg gttaattcat tcctgttcat
     2041 ccctcatccc tcatccctca tcccttgatt tttatgtctg ccacactact cgaaaatcct
     2101 ctgcgcgcgg gcgtgcgcct cgaacggacg gcggagccgt gcatcgtcgt catcttcggc
     2161 gcgaccggcg atttgacgaa gcgcaaactc gtgcccgccc tctatcgtct cgtgcaggag
     2221 cgactcgtgc ccgccgagtt tgccatcgtc ggcgcggcgc gcaccgagat gacgagcgaa
     2281 gaatttcgcg ccaacatgcg tgaagccgtc gtccagtttt cggaagccaa gcgcgttgac
     2341 gaagaggtgt gggaatcctt cgcgcagggc ttgcactatt tgagagttga catcggcgca
     2401 agcgcagact atgtgacgct cggcgcgaaa ctcgacgaga ttgacgcggc gcgcggcacg
     2461 cagggcaacc gcgttttcta tctctccacc gcgccttcgc tctacgcgga agccgtccgg
     2521 caactcggcc agtcgggctt ggcgaaaccg aaagagaagg gctgggtgcg cgtcatcatc
     2581 gagaagcctt tcggcacaga cctcgccagc gcgcaggctc taaacagaga cattcaccag
     2641 cacctcgacg agtcgcagat ttatcgcatc gatcattatc tcggcaaaga gacggtgcaa
     2701 aacctgctcg tctttcgttt cgccaacggc atctacgagc cgctctggaa ccgccagtac
     2761 atagaccacg tgcaactcac gaacgccgag acggtcggcg tcgaaggtcg cggcggctac
     2821 tatgaaaagg ctggcgtggt gcgcgacatg ttgcagaacc acgtctttca agtgctctcg
     2881 ctcgtggcga tggagccacc cgcgagtctc aattccgaat cggtgcgcga cgagaagatc
     2941 aaggcgatga cctccgtgcg cccgtttacg cccgaacgca tccgcgccga atgcgtgcgc
     3001 ggtcaatacg gagccggttc gatcaacggc aaacccgttc ccggttatcg tgaagaagag
     3061 ggcgtcgcgc cggattccgc caccgaaact tttgcgatgc tcacgatgta tttcgacaac
     3121 tggcgttggt cgggcgtgcc cttctacata cgttcgggca agcgtctggc aaagcgcgtg
     3181 accgagatcg cgatccagtt taaagacgcg ccgcacaaac ttttcggctc cgagatgatg
     3241 gagcagatga cgccgaacca actaatcatc cgcgtccagc cggacgaagg catcaccatg
     3301 cgcgtcgctg cgaaagtgcc cggacaggta acgcgcatcc gcgacgtgaa catggatttt
     3361 cgctacggct catccttcgg cgtccaactc gccgaagcct atgagcgact cctgctcgat
     3421 tgtattctcg gcgacccgac tctctacgcg cgcaccgaca tgaccgagcg cggctgggac
     3481 atcgtcatgc cgattctcga cgaatgggcg gccacgaaag cggacgcgcc cttcccgaac
     3541 tacgaagccg gaacgtgggg accgcaagcc acacacgagc ttctggaaaa ggaaggccgc
     3601 cgctggcgca gaccctagaa gggcagtgac aggtgacgag tgacgagata aaaccgtgaa
     3661 tcggcacctg tcactcacca cccggcacct gtcacttgtc gctcgtcata cgtgaatagc
     3721 caaaaccagt tttaatctat ctgcgattca cgatttactt ttttcatctg tcacttgtca
     3781 cctgtcactt gtcactgtct tcttatgagt agagagattc cacagagcac agccgccggg
     3841 gatgtagcgc agggcattga tgtcgtgcgt ttggagaaag agttggcggc gagttggcaa
     3901 aatgaggcgg gcgcgggtga gtccggcgtg acgcgcgtgt gcgtgttgaa tttgatcgtc
     3961 tatgccacgc ctgccgatga ccgcgcgaag atcgatgccg tgctcaacga ggtgacggcg
     4021 cacacgccga gccgcgcgct cgtgctgatt gctgatcgtg tctcgacgga agcgaggttg
     4081 gaagccagcg tctccgcgct ctgccagcag gagagcaggg gcggcgcaaa gcgcgtgtgc
     4141 ggcgaacagg tggtgatcga ggcgagcggg gcgcagattg agacgactgc gagcgcgatt
     4201 gagccgctgg tcgtgccgga tgtgccggtc tttctctggt ggaaagatat tccgcacgag
     4261 gaagataaac tgttcaatcg cctcgtcgag ttgtcagacc gcgtcgtgat cgattcgctt
     4321 gtcttcgacg agccgcacga agacctgaag cgtttggcgc aactcatacg cgagcggcgg
     4381 cagtatcttc tgttgagcga cgtgaactgg ggacggctca cctcctggcg caacttgatc
     4441 gcgggtttct gggacgtggc agactatcgc gcgcacctcg acgcgctcga ccacgttttg
     4501 attgaattcg atccgcccga taattcgtcc ggcgagatcg cggcgcaagc cctgctcgtt
     4561 ctcggctggc tggcgtctcg tctgaagtgg caggcgacgg gtgagttcaa gcgagagggg
     4621 cagagcgcgg tctggatgtt gacggcgggc gagcgcaaac tcagagtcga attgcgcgca
     4681 aacgacgaca ggcacggttg cgatagtctc atcgcgggcg tcacgctcaa gtctgcgacg
     4741 agtggcgcgg agtttttcgt cggggtgaat gaagagtgga caaagctcga aacctcggcc
     4801 aagattggcg acgcacacac cgtcgggcgc gtcgtcactt acgaagcgaa gtccgaaggc
     4861 gagcggctca gtcgtgaact cgggatgctc gcgcgcgatg tgatctatga acaagcggtt
     4921 gcgtcggtcg ggcaattgct cgaaggcttg cagaaaagct agtcgaatct gaaagaatat
     4981 ttttcaacct catgatat
//
LOCUS       DASWNS010000509         9937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_487523, whole genome shotgun sequence.
ACCESSION   DASWNS010000509 DASWNS010000000
VERSION     DASWNS010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487523"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VGB76_17560"
     CDS             <1..427
                     /locus_tag="VGB76_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100757.1"
                     /translation="EYDPKTGEPKTVKKYERDRRGNIIREYRQIVISEEKRIDSWFLC
                     IAPLDYPQLAMAVIVEGGGYGSRAAAPIAAALVLKAKELGLLGNIQGAPDGQQQPQQQ
                     PQAQPNGGRQPSSPARPGQQPQSRPTQPKPTPRAVAVRQ"
     gene            506..2224
                     /locus_tag="VGB76_17565"
     CDS             506..2224
                     /locus_tag="VGB76_17565"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0100758.1"
                     /translation="MKNRASTHLFILLLIVGLQIAGYVAVTRAAILRGYQPSTVSAVR
                     DLLLYVPIFFLVLWLARQMKFRGNWALYTTAILLFSLGMLVQYRLYSDPEYGSRNKAE
                     ARAEKTQVQRLRFINKFYDDDKKRLLGFSTNREQVESQLIDQAEEQSNVTVGRVLASG
                     FTWIPILAFVAFGVAYYFCMRDEFLNWVQRHSFIIVLITLVPLVAAVVTSSAGKALGN
                     MTPWEPSKIPFLLGFAGILTAYYRDLAKTYWGMPRAQAIIPLIVMALIPFIPFFALKD
                     FGQMLVFSGAYATLYLVAVRRWPQLLVFVGSVVLVIGVLVAGSLPPNVQEKVPLLPTL
                     SRPVQAALPARIKQRFHLWLDGFNPPSPNESWWKKDYDDALANDGRMRELAESSEAMK
                     ETVNVDVWFDRIAFQPAQATFGIASGGLTGRGLGLGFAEVIPVADSDYIYAAIGEELG
                     LAGGALVILALIVFVNAGVRTAIDARDMFTKLCSAGLTAFIGFQALVNIGGITRALPM
                     TGITLPFVSHGGFSLITSFAMLGMLMAFSNRNARDAAATPASNPRPSSPASSTAREPF
                     IPEAVE"
     gene            2221..3876
                     /locus_tag="VGB76_17570"
     CDS             2221..3876
                     /locus_tag="VGB76_17570"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY0100759.1"
                     /translation="MSEQSAGVRVEVASVTDRGLSQKRPLNEDSMLVDAERGIFAVAD
                     GVGGAQAGEVASQTAVEVLGEAFRHHKDGDDIEDLMEIAIQRANASIYQMSHEQRQLS
                     MMATTIVALHLDGRQATIGHVGDSRLYRLTPEGRLERETEDHSVVEEEVRAGRMSPEQ
                     AAHHPSRNVISRALGAEQAVEVDLKTMEVEDGTIFLLCSDGITRHIADEELSALLRGG
                     GASLDAACAEMKRLCFERGAEDNLTAVVVRVGSASHASRETLTTDEDERTLLTERSTA
                     RLSAGANKTDHVAAASTTTMFQTPLKGAFAQDLPDTHATPRAATNSKLRIDTEELSTA
                     AAPTATPQPARAQTVRSPTGRASSWFGVLLLLLIASAAAFYGGMRYQQNRGDAASVAA
                     GTGGNANPPAAPVTTQATPAPASELNINDTYTRLKAAVDSSPTAEAQRMAGENGGKPL
                     DSTDGVFLALYGRALFLSGKYPEAVEALQRANNKIDAQFAPGRDPVRLDTRMTLAAAA
                     LRAGNQEAIRVASDSFGGVIEEPGTGASTVPTVDAAPAPQQPR"
     gene            3930..5540
                     /locus_tag="VGB76_17575"
     CDS             3930..5540
                     /locus_tag="VGB76_17575"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0100760.1"
                     /translation="MSELKLQQCRLDGRYDILECLGRGSYAEVYLARDAAASGGSPQM
                     VVIKALNLFLQGAIDLELEHTLIENFRNEAVALDRVRHPNIINRLGHGTAIDMAGTTF
                     HYLVLEYLPGGDMAALCRSHPLTLDRALYYLDQICDGLAHAHEHDVIHRDIKPQNLLL
                     TADRQVVKIADFGVAKIEATEGLITRVGTDVYAAPEHHPLMQAGVLGTGSLTSPVTQL
                     TPAADVYSLAKTAYMLLTGEAPRRFSQRPITELPAQMAAEPWASFVLRVLRRGTETNP
                     AKRYQSVREFWNELSDALMPKTQLLTQPNGEETRPLSQVGGAQRVVPLPPEPSPAQFE
                     RQVPQPQQGGGAGKAHPRIVVPVALAQPPTPQTNIAEAAAALAQTPTGHKPQTSPQAD
                     APARPKGRGTMMPRKTWFTEHARPWIVALLLLAGFALLLLGIQYQVRSRQRSTTPANA
                     STGQGAPAPNSGTLQTGREMTTTTNVNLRSGPSRTYNRIGEVESGSRVRILQVSGNWC
                     EVLILQRGFPRRGEESAEQGWIDGTRLR"
     gene            5615..6460
                     /locus_tag="VGB76_17580"
     CDS             5615..6460
                     /locus_tag="VGB76_17580"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0100761.1"
                     /translation="MSILESIRKWIDGEGEEDPLASVDEQAKPRRVWEEFLVKVAREI
                     EIVMQREMFTPPGGQTYIPPEYIVYLSGEDDKDWQGEKRRGLEQGLFYVLSERARELS
                     GSTQLATKSFAVELRVDGTLEKGQFRVQPVWDATESGHTMVTARAPEVGATTTPDAAL
                     QEAEPNESEVTRVSARVVELFAVELWRGGVRQQVVPITKPEISIGRGSKSVAVDLPIK
                     GDPEVSRVHAMLSRDPEGKFWLTAKGRNATLVSGVEAPRDESVPVAPDDKIEICSYTL
                     RIQPR"
     gene            6536..6832
                     /locus_tag="VGB76_17585"
     CDS             6536..6832
                     /locus_tag="VGB76_17585"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEY0100762.1"
                     /translation="MTYIVTEACVDCKYTDCAAVCPVEAFHELPDRLYINPETCIDCD
                     ACVPECPVEAIFSDAALPVEFQEWVELNAEADQHPIISGKIDALLGPGCKPAEQ"
     gene            complement(6940..8214)
                     /locus_tag="VGB76_17590"
     CDS             complement(6940..8214)
                     /locus_tag="VGB76_17590"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0100763.1"
                     /translation="MSDQTTKLIHAFSALADLGEELADTRDFEEMVRSTLHVVLGSLG
                     VRRGAVAEYVQTDGLLRFVAAWGLGEGTPEKFPLDFGNAELFCTADPRALSLADQEQL
                     ALVEARLGAQLTDALRRLHVSLVVPLVVHKKFVGVILLGSKATEESFTADEREVVRTM
                     ARHIGVGIYNHRLLREVEQRAEENRGLYEDLRAIYRDTVRAFAAAIDCKDKYTQGHSE
                     RVGKYSEIIAREMGWSEECVEGIAVAGYLHDIGKLVVERDIINAPYKIDAKQSSELNR
                     HPAAGYEILAPINHPFADIPLMAKYHHERIDGRGYPEGLTGDQIPPGAKIVSVADSFD
                     AMTTDRTYKRRKVFTEVVEDFRKNAGKQFAPEVLAALCRALLKEVNGETKERHMTKLL
                     GKGYLEAEVCAPLLVELIAELESGVTLAASSA"
     gene            complement(8219..8623)
                     /locus_tag="VGB76_17595"
     CDS             complement(8219..8623)
                     /locus_tag="VGB76_17595"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0100764.1"
                     /translation="MVNSHDRSPVRSGRSGDIAIIYASDYLNKLSGERIERECRAHLG
                     AGCRALIVDFKNTGLVNSIGVSIMLGVINAAERAGAPLVFSNVNAQAAQLFEILGLTR
                     HAALAHDETDALALLSEVNFPLQPSAPVGRHS"
     gene            complement(8640..>9937)
                     /locus_tag="VGB76_17600"
     CDS             complement(8640..>9937)
                     /locus_tag="VGB76_17600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0100765.1"
                     /translation="SLTIRRALIRLLHDAATNAGGKSPVEAWRRRLELEPDELYPLMF
                     ALHVRELASFNATFMEIGTESLWRDYLRASYRLQVAAEPRALVVADTLVETLKRAPQT
                     MARHYRRAAALDLRELLARFDEQRVASSLLHYDRFSRIYRGIETEEIELGLDAETDLI
                     RLPQIVHAASCASFHPPMLHVCDEERSAVAHGFDAMPYTDANEVVWITAEIDAKLEAG
                     RAATEIWCDRLTQVGHACGFKRFKLWLVAPEGFSAEASELLNERNAFSSSRKQLELLT
                     ARLSPETTDARAAAEADAPDEFEMVIPMGSDTELIAAHTVEQIARRLNFQPEAINQIK
                     TALIEACINAAEHSLSPDRKIYQRFRLESDKLVVTVSSRGIIVPSPTNAGTNGSEARE
                     ENNGRRGWGLKLIRTLMDEVEFERVDDGTRLRMVKYLHR"
BASE COUNT         1877 a         3132 c         3115 g         1813 t
ORIGIN      
        1 ggagtatgac ccgaagacgg gcgagccgaa gaccgtcaag aagtatgagc gcgaccggcg
       61 gggcaacatc atacgcgagt acaggcagat cgtgatctcg gaagagaaac gtattgacag
      121 ttggtttctc tgcatcgcgc cgctcgatta cccgcaactc gcgatggccg tgatcgtcga
      181 aggcggaggc tacggctcgc gcgccgccgc gcccatcgcc gccgcgctcg tcctgaaagc
      241 gaaagaactc ggactgctcg gaaacattca gggcgcgccg gacgggcagc aacaaccgca
      301 gcaacaaccg caggcgcaac ccaacggcgg gcgtcagccg tcttcgcccg cgcgccccgg
      361 acagcaaccg cagtcgcgcc cgacgcagcc gaagccgacg ccgagggctg tcgccgtcag
      421 gcaatagaag gagttcatca gccgcgaacc gctgttcgtt cgcgtggaac attgttgagt
      481 ggaatatttg tagtcccgac cagttatgaa aaatcgtgcc tcgacacatc tctttatcct
      541 gctcctgatc gtaggcttgc agatcgccgg ttatgtggcc gtgacgcgcg ccgcgatcct
      601 gcgcggctat caaccttcga ccgtgagcgc ggtgcgcgat ctgttgctct acgtcccgat
      661 cttcttcctc gtgctctggc tcgcgcggca gatgaagttt cgcggcaact gggctttgta
      721 cacgacggcg atcctgctct tctcgctcgg catgctcgtg cagtacaggc tctacagcga
      781 tcccgaatac ggctcgcgca acaaggccga agcacgcgcc gagaagactc aggtgcaacg
      841 cctccgcttc atcaataaat tttacgacga cgacaagaag agactgctgg gcttctccac
      901 gaaccgcgag caggtcgaat cacaactcat cgatcaggcc gaggagcagt cgaacgtgac
      961 ggtcggacgc gtgctcgctt ccggctttac atggattccg attctcgcct tcgtcgcttt
     1021 cggcgtcgct tactatttct gtatgcgcga tgagtttctc aactgggtgc agcgccactc
     1081 cttcatcatc gtgctcatta cgctcgtacc gctcgtggcg gcggtcgtca cttcaagcgc
     1141 gggcaaggcg ctcggcaaca tgacgccttg ggaaccatcg aagattccct tcctgctggg
     1201 cttcgccgga atcctcacgg cctactaccg cgacctcgcc aaaacttact ggggcatgcc
     1261 gcgcgcacag gcgatcatcc cgctcatcgt gatggccttg atccccttca tcccgttttt
     1321 cgccctgaaa gacttcgggc agatgctcgt cttcagcggc gcatacgcga cgctctacct
     1381 cgtcgccgtt cgccgctggc cgcaactctt agtcttcgtc ggctcggtcg tcctcgtcat
     1441 cggcgtgctc gtcgccgggt cgctcccgcc gaacgtgcag gagaaggtgc ctttgctgcc
     1501 gacgctctcg cgcccggtgc aggccgcgct gcccgcgcgc atcaagcagc gtttccatct
     1561 ctggctcgac ggcttcaacc cgccctcgcc caacgaatca tggtggaaga aagattacga
     1621 cgacgcgctc gccaatgacg ggcggatgcg cgaactcgcc gaatcgagcg aggcgatgaa
     1681 agagacggtc aacgtagatg tctggttcga ccgcatcgcg tttcagcccg cgcaggccac
     1741 gttcggcatc gcgtcgggcg gactcacggg tcgcggcctc ggactcggtt tcgcagaagt
     1801 gatcccggtc gccgactccg actatattta cgcggccatc ggcgaagaat tagggctggc
     1861 gggcggcgcg ctcgtcatcc tcgccttgat cgtctttgtc aacgcgggcg tccgcacggc
     1921 gattgacgcg cgcgacatgt ttaccaagct ctgctcggcg ggcttgacgg ctttcatcgg
     1981 ctttcaggct ctcgtcaaca tcggcggcat cacgcgcgcc ctgccgatga cgggcatcac
     2041 gctcccgttt gtgagccacg gaggattcag tttgatcacg agttttgcca tgctcggcat
     2101 gttgatggct ttttccaatc gcaacgcgcg cgatgccgcc gcgactcccg cgtcgaaccc
     2161 gcgcccgtcg tcgcccgcgt caagcaccgc cagagaacct ttcatcccgg aggctgttga
     2221 atgagtgagc aatcagccgg agtaagagtc gaagtcgcgt ccgtcaccga ccgtggactc
     2281 agccagaagc gaccgctcaa cgaagactcg atgctcgttg acgcggagcg cggcatcttc
     2341 gccgtcgccg atggcgtggg cggcgcgcag gcgggcgaag tcgcatcgca aaccgccgtc
     2401 gaagtgctcg gcgaagcctt ccggcatcac aaggacggcg acgacatcga agacttgatg
     2461 gagatcgcca tccagcgcgc caacgcttcg atctaccaga tgtcgcacga gcaacggcaa
     2521 ctctccatga tggcgactac catcgtcgcg ctgcacctcg acggcagaca ggcgaccatc
     2581 gggcacgtcg gcgattcgcg cctctatcgc ctcacgcccg aagggcgttt ggagcgcgag
     2641 acggaagatc attcggtggt ggaggaagag gtgcgcgccg ggagaatgtc gcccgaacag
     2701 gcggcgcatc atccgagccg caacgtcatc agtcgcgcgc tcggcgcgga gcaggccgta
     2761 gaagttgacc tcaagacgat ggaagtggaa gacggcacga tcttcctgct ctgctccgac
     2821 ggcatcacgc gccacatcgc ggacgaagag ttgagcgcgc tgctgcgcgg cggcggcgcg
     2881 tcgctcgatg cggcctgcgc ggagatgaag cggctctgct tcgagcgcgg cgcggaggat
     2941 aatctaaccg ccgtcgtcgt gcgcgtcggt tccgcgtcac acgcctcgcg cgagactttg
     3001 acgacggacg aagacgaacg cacgctcctg accgaacgct cgacggcgcg actctctgcg
     3061 ggcgcgaaca aaacagacca cgtggcggcg gcgtcaacca cgacgatgtt ccagacgccg
     3121 ctcaaaggcg cgttcgcgca agacctcccc gacacgcacg ccacgccgcg cgccgccacc
     3181 aacagcaagc tgcgcataga cacggaagaa ctctcgaccg ctgccgcgcc gacagcgacg
     3241 ccgcaacccg cgcgcgcgca gaccgtgcgt agcccgacgg gtcgcgcgag cagttggttc
     3301 ggcgttctgt tgttactctt aatcgcatcg gcggctgcgt tctacggggg catgcgttac
     3361 cagcagaacc gtggcgacgc agcatcggtc gccgcaggca ccgggggcaa cgcgaatccg
     3421 cccgccgcgc ccgtcacgac ccaggcgacc ccggcaccgg ccagcgaact gaacatcaac
     3481 gacacctaca cgagattgaa ggcggcggtt gactcgtcgc ccacggcgga agcgcagcgg
     3541 atggcgggcg agaacggcgg caaaccgctg gactcgaccg acggcgtctt tctcgctctc
     3601 tacggtcgcg cgctcttttt gtcgggcaag tatccggagg cggtcgaagc cttgcagcgc
     3661 gcgaataaca agatcgacgc tcaattcgcg cccggacgcg atcccgtacg cctcgacacg
     3721 cgcatgacgc tcgcggcagc cgccctgcgc gcgggcaatc aggaagccat tcgcgtcgct
     3781 tccgattcgt tcggaggcgt catcgaagaa ccgggaacgg gcgcgtcaac cgtgcctaca
     3841 gttgacgcgg ctcccgcgcc gcagcagcca cgttaagcgt ccgggttcgt tttgaagatg
     3901 caggattaag taaagattgc gaacaacgca tgagtgaatt gaaacttcaa cagtgcaggc
     3961 tcgacgggcg ctacgacatt ctcgaatgtc tggggcgcgg cagctatgcc gaagtttatc
     4021 tcgcgcgtga tgcggcggcc tccggcggct cgccgcagat ggtcgtgatc aaagctctca
     4081 acctcttcct gcaaggcgca atcgatctcg aactcgaaca cacgctcatc gagaattttc
     4141 gcaacgaagc cgtcgcgctc gaccgcgtgc gccacccgaa catcatcaac cgtctcggcc
     4201 acggcacggc gatagacatg gcgggcacga cgtttcacta tctcgtcctc gaatatttgc
     4261 ccggcggcga catggccgcg ctctgccgca gccacccgct cacgctcgac cgcgccctct
     4321 attatctaga ccaaatctgc gacggcctcg cacacgccca cgagcacgac gttattcacc
     4381 gcgacatcaa accgcagaac ctgttgctca cggctgacag gcaggtcgtc aagatcgcgg
     4441 acttcggcgt cgccaagatc gaagccacgg agggcttgat cacgcgcgtc ggcacggacg
     4501 tttacgccgc gccggaacat cacccgctca tgcaggccgg ggtgctcggc acaggctcgc
     4561 tcaccagtcc ggtgacccaa ctcacgcccg ccgcagacgt gtactcgctc gccaagaccg
     4621 cttacatgct tctgaccggc gaagccccca gacgcttctc gcagcgaccg atcacggaac
     4681 tgcccgcgca gatggcggct gagccgtggg cttccttcgt cctgcgcgtt ctgcgtcgcg
     4741 ggacggagac gaatccggcg aagcgttatc aaagcgtgcg cgagttctgg aacgaactga
     4801 gcgatgccct gatgccgaag acgcaattgc tgacgcagcc gaacggcgaa gagacgcgcc
     4861 cgctctcgca ggtcggcggc gcgcagcgcg tcgtgcccct gccgcccgaa ccgtcgcccg
     4921 cgcagttcga acggcaggta ccacaaccgc agcaaggcgg cggcgcggga aaagcgcacc
     4981 cgcgcatcgt cgtgcccgtc gcgctggcgc agccgcctac cccgcagacg aacatagcgg
     5041 aggcagcggc ggctttggcg cagacgccga ccgggcacaa gccgcaaacg tcgccgcaag
     5101 cggacgcgcc cgcaaggccg aaggggcgcg gcacgatgat gccgcgtaag acctggttta
     5161 cggagcacgc gcgcccgtgg atcgtcgcgc tgctcctgct ggcggggttc gcgctgctgt
     5221 tgctggggat tcaatatcaa gtacgcagcc gtcaacgcag cacgaccccg gcaaacgctt
     5281 caaccgggca gggcgcgccc gcccccaact ccggcacttt gcagacgggg cgcgagatga
     5341 cgacgaccac aaacgtcaac ttgcgatcag gaccgagcag aacttataac aggatcggcg
     5401 aggtggagag cggttcgcgc gtgcgcatcc tgcaagtgag cggcaactgg tgtgaagtgt
     5461 tgatactcca gcgtggcttt ccgcgacgcg gtgaagagtc cgccgagcag ggctggatcg
     5521 acggcacaag gttaagatga gttctcattt aagatcagtt ttcataaacg ccggagcgag
     5581 ttgttggcgc aaatgatcga gaggtggatg aaacgtgagc atactggaat ccatacgcaa
     5641 gtggattgat ggcgagggcg aggaagaccc gctcgcctcc gttgacgaac aggcgaaacc
     5701 gcgccgcgtc tgggaagagt ttctggtcaa ggtggcgcgc gagatcgaga tcgtgatgca
     5761 gcgcgagatg ttcacgccgc ccggcgggca gacctatatc ccgcccgagt acatcgtcta
     5821 tttgagcggc gaagacgaca aggactggca gggcgagaaa aggcgcggac tcgaacaggg
     5881 actcttctat gtgctctccg agcgcgcccg cgaactgagc ggttccaccc aactcgccac
     5941 caaatcattc gccgtcgaac tgcgcgtgga cggaactctg gagaagggtc agtttcgcgt
     6001 gcagcccgtg tgggacgcga ccgagagcgg ccacacgatg gtcacggcgc gcgccccgga
     6061 agtcggggcg acgacgacac cggatgcggc gttgcaggag gccgagccga acgaaagcga
     6121 ggtcacgcgc gtcagcgcgc gggtcgtcga gttgtttgcg gtcgaactgt ggcggggcgg
     6181 cgtgcgtcaa caagtcgtac cgatcacgaa gccggagatc agcatcgggc gcggctcgaa
     6241 gtccgtcgcg gttgacttgc cgatcaaggg cgacccggaa gtgagccgcg tgcacgcgat
     6301 gctctcgcgc gaccccgaag gcaaattctg gctgaccgcc aaagggcgca acgccacact
     6361 cgtctccggc gtggaagccc cgcgcgacga gagcgtgccc gtcgcacccg acgacaagat
     6421 cgagatttgc agctacaccc tgcgcatcca gccgcgctga tggaggcgcg gcgcgcccgc
     6481 tcacgaaccc gtgcgcgaaa gattttctgc gaattgtttg aggagataat tgacgatgac
     6541 gtacatcgtg accgaggctt gtgtcgattg caaatacacg gactgcgcgg cggtctgccc
     6601 cgtcgaagcc ttccacgaat tgcccgaccg cctctacatc aacccggaga cttgtatcga
     6661 ttgcgacgcg tgcgtgccgg agtgtcccgt cgaggccatc ttctcggatg cggcgttgcc
     6721 cgtagagttt caggagtggg tggaattgaa cgccgaggcg gatcagcatc ccattatcag
     6781 cgggaagata gacgcgctgt tgggaccggg gtgcaaaccg gcggaacaat aaatcagcgg
     6841 aacaataaat caaacgatga agtaggaacg cggaacggtg aatgagaagc agttagcttt
     6901 cagttcatcg ttccgcgttc atatttcatc ctctcttttt tacgcgctgc tcgcggcgag
     6961 cgtcacgccg gattcgagtt cggcgatgag ttcgacgagg agcggcgcgc agacttcggc
     7021 ttcgaggtag cctttgccga gaagtttcgt catgtgccgc tctttcgtct cgccgttcac
     7081 ttccttcaac agcgcacggc acagcgcggc cagaacttcg ggcgcaaact gcttgcccgc
     7141 gttcttgcga aaatcttcga cgacctcggt gaaaactttt cgccttttgt aagtccggtc
     7201 ggtcgtcatc gcgtcgaacg aatcggcgac cgagacgatc tttgcgccgg gcggaatctg
     7261 atctccggtc aaaccttccg gatagccgcg cccgtcgatt cgctcgtggt gatacttcgc
     7321 catcagcgga atgtctgcga aaggatgatt gatgggagcg agtatttcat aaccggcggc
     7381 ggggtggcgg ttgagttcgg acgactgctt ggcgtcgatt ttgtacgggg cgttgatgat
     7441 gtcgcgttcg acgacgagtt tgccgatgtc gtggaggtag cccgcgacgg cgatgccttc
     7501 cacgcactct tcgctccaac ccatctcgcg cgcgatgatc tcgctgtact tcccgacgcg
     7561 ttccgaatgt ccctgcgtgt atttgtcttt gcagtcaatc gccgccgcaa acgcacgcac
     7621 cgtgtctcga tagatcgcgc gcaaatcttc gtagaggccg cgattctctt ccgcgcgctg
     7681 ttccacctcg cgcagcaacc tgtgattgta aatcccgacg ccgatgtggc gcgccatcgt
     7741 gcgcaccact tccctctcgt ccgccgtgaa actctcttcc gtcgccttgc tgccgagcag
     7801 gatcacgccg acgaattttt tgtgcaccac gagcggcacg acgagactga catgaagccg
     7861 tcggagcgcg tccgtgagtt gcgcgccgag gcgtgcttcg acgagcgcga gttgttcctg
     7921 atcggcgagc gagagggcgc gcgggtctgc cgtgcagaaa agctcggcgt ttccgaaatc
     7981 gagcggaaac ttctccggcg ttccttcgcc gagtccccac gcggcgacga agcgcagcag
     8041 cccgtccgtc tgcacgtatt cggccaccgc gccgcgccgg acgccgagcg aaccgagcac
     8101 gacatggagc gtggaacgca ccatctcctc gaagtcgcgc gtgtcggcga gttcttcgcc
     8161 gaggtctgcg agcgcggaga aagcgtgaat cagtttagtc gtctggtctg acatcgcctc
     8221 aggaatgcct gccgaccggg gcggaaggct ggagcgggaa gttcacttct gagagcaggg
     8281 caagcgcatc cgtctcatcg tgcgcgaggg cggcgtggcg cgtgaggccg agtatctcga
     8341 agagttgcgc ggcctgcgcg ttgacgttgg agaagacgag cggcgcgccc gcgcgctccg
     8401 ccgcgttgat cacgccgagc atgatcgaga cgccgatgct gttgaccagc cctgtgtttt
     8461 tgaaatcgac gatgagcgcg cgacaaccgg cgccgagatg cgcgcgacat tcgcgctcga
     8521 tccgctcgcc gctcagtttg ttgagatagt cgctggcata aatgattgcg atgtccccgc
     8581 tgcgcccgga gcgcaccgga gaacggtcgt gcgagttaac catttttttg cggccccttc
     8641 taacggtgta ggtacttcac catccgcagg cgtgtgccgt cgtcaacgcg ttcgaactcg
     8701 acttcatcca tcagagtgcg aatcagtttt agaccccagc ctcggcgtcc gttattctct
     8761 tctcgtgctt cgctgccgtt ggtgccggcg ttcgtggggg agggcacgat aatacccctg
     8821 cttgatacgg tgactactaa tttatcactt tcgagacgaa aacgttgata tatttttcgg
     8881 tcgggactca ggctgtgctc cgccgcgttg atacaggctt cgatgagggc ggtttttatc
     8941 tggtttatgg cctcgggctg gaagttcaga cgccgcgcga tctgctcgac ggtgtgcgcg
     9001 gcgatcagct ccgtatcgct ccccatcggg atgaccatct cgaattcgtc cggcgcatcc
     9061 gcttccgcag ccgcgcgtgc gtccgtcgtt tcggggctga ggcgcgccgt caggagttcg
     9121 agttgcttgc ggctcgaact gaaggcgttt cgctcgttca acagttcgga agcctccgcc
     9181 gaaaatcctt cgggggcaac cagccacagc ttgaagcgtt tgaagccgca ggcgtggccg
     9241 acctgcgtga gacggtcgca ccagatttcg gtggcggcac gcccggcttc tagtttggca
     9301 tcgatctcgg cagtgatcca gacaacttcg ttggcgtccg tgtagggcat cgcgtcgaag
     9361 ccgtgcgcga cggcggagcg ttcttcgtcg cagacgtgca gcatcggcgg gtgaaacgag
     9421 gcgcaactcg ccgcgtggac gatctgcggc aggcgtatca gatcggtttc ggcatcgagt
     9481 ccgagttcga tctcttccgt ctcgatgccg cgatagatgc ggctgaaacg gtcgtagtgc
     9541 aggaggcttg aggcgacgcg ctgctcgtcg aagcgtgcga gcagttcgcg caggtcgagc
     9601 gcggcggcgc gacgataatg gcgcgccatc gtctgcggcg cgcgtttcaa agtctcgacc
     9661 agcgtgtcgg ccaccaccag ggcgcgcggt tcggcggcga cttgcaggcg ataactcgcg
     9721 cgcaggtaat cgcgccagag gctctccgtg ccgatctcca tgaaggtggc gttgaagctg
     9781 gcgagttcgc gcacatgcag tgcgaacatc aaagggtaga gttcgtccgg ctcaagttcc
     9841 agacgcctgc gccacgcctc gacgggcgac ttgccgccgg cgttcgtcgc cgcgtcgtgc
     9901 agcaaacgga tcagcgcgcg gcggatggtg agcgagg
//
LOCUS       DASWNS010000510         6599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_97006, whole genome shotgun sequence.
ACCESSION   DASWNS010000510 DASWNS010000000
VERSION     DASWNS010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97006"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..2150)
                     /locus_tag="VGB76_17605"
     CDS             complement(210..2150)
                     /locus_tag="VGB76_17605"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0100766.1"
                     /translation="MNLTGRSNKFSQTSKLAAFLLLATLCVWFFPSAPASANSVREKK
                     DKVAGDLREKIGKARNGEQIKVIIQPSGAWSQSLDSDITYHNGNTTRKYSHFNARALT
                     LPAPAVHALAERADVEYISLDREVKQLGHVTLTSGADAARSMGGTGTTLDGSNTGIVI
                     IDSGIDPNHTAFLDAASKSRIVYQQDFTGENRTDDPYGHGTHVAALAAGNGLVSEGAY
                     RGIAYNSRIINLRVLNAQGRGTTSSLLGALDWVYANRANAAYKIKVVNLSLGTAAVDS
                     YKYDPLCRAARRLVDAGIVVVAAAGNEGTDSAGRKIYGQIHSPGNEPSVFTIGAANSL
                     GTNSRADDLVTTYSSRGPTRSFWTDSKRIRHYDNLLKPDLVAPGNKLIAAQSAGNYLV
                     AGNPALDANVSFIATREQMYLSGTSMAAPVVAGGAALLLQANPSLTPNLAKTILMYTA
                     QPLAGFNHFEQGAGELNIEGAIRLAKAMRTDVSNAALPLGAPLLCYNCALPTPQTTIS
                     GRTFQWSQGLILGHTYASGSNLVTRYQKIYDQGTLLGAGLSESSGVLIGDGVLIGDGV
                     LIGDTILTSYGATIGEGSFLFGTGVLIGDGVLIGDGVLIGDGVLIGDGVLIGDGVLIG
                     DFRAQAKLALTNGDNTSCMTVK"
     gene            2462..3298
                     /locus_tag="VGB76_17610"
     CDS             2462..3298
                     /locus_tag="VGB76_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100767.1"
                     /translation="MKDFEVLPEEVEIYRDRMWQREPESRVENALEAEKLIERAGFCA
                     AMTDARRPGASLYVSVCGRRDAHMPRNVQKDPESSQTWLLKDEIMRRGRVYYAKLAKA
                     RALFVAPRLVPHFNALWGVARREESARLSAEARAVLKILRREWEMATCDLRTTAKLGE
                     RAQLTRALDELQRTLKVIPCDVLYEPWFTYIWTLAEGRFAAELKVKVKREVALREVAR
                     AFLGGAGRTVRGELAKVTGLSRPDAGLGNRALVAEGYAVRLSEGVYQLADFEQRFREM
                     NN"
     gene            complement(3333..4139)
                     /locus_tag="VGB76_17615"
     CDS             complement(3333..4139)
                     /locus_tag="VGB76_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100768.1"
                     /translation="MSDKRNSTGNRLTAKERATHFLLQTFPWLAFLIIALPVPLYFLF
                     RYFNAASGAEAGEFMLLAMTTLAAGSIIGLVAVVLLFIYRRQWEKRLRNRLAADGVTT
                     DELSWFTAELTKAERRTLREIETRNLLLADAYRETLATRITAARVAANARREAVSVER
                     RIQNTARLGVAERTTLEEELKGDRERLGRIEAEATERRAQAEARLLMIEAAAGRNASD
                     EETRIALQRLELAAAQVPHSLETARLEREAREQVEQELRADRQLPGGGTN"
     gene            4222..5211
                     /locus_tag="VGB76_17620"
     CDS             4222..5211
                     /locus_tag="VGB76_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100769.1"
                     /translation="MKKGLPLRLLLLFVLACAAVADAETYRDADGQKGDGNGARRAET
                     PRSVRAVTIPVTVKLRDERPASEDLRVLDLFVTEDGERQEILSTRSAADHAPLALTLL
                     VQDDLASPIGLEIGGLANFIRNLPQGSHVQVGYLRSGSLQLRQKFTTDLERAAKSLRI
                     PIGSASAAPYNPYVQIYDSLKRYGGVPLGTRRAVLVISDGLDLSRGFDSSSPTHSIDL
                     DRAISEAQRRSVAIYGIYAPTVGQGGSTALVSNAQGSLLRLTQETGGKAFFQGTGAAV
                     SFAPFLRELSDRLARQFALTYLSTHPDKGFHRIKIDSPGTEAELAYPVGYTRK"
     gene            5353..>6599
                     /locus_tag="VGB76_17625"
     CDS             5353..>6599
                     /locus_tag="VGB76_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0100770.1"
                     /translation="MYFKQFYLGCLAHASYLIGSEGEAAVVDPQRDIEQYIAESAAQN
                     LRIKYVVETHLHADFVSGHCELAARTGAEIVFSRHARAAFPHRAVQDGDEIELGRVKL
                     RFWETPGHTPESLCVLVIDTDVSDEPQKVLTGDTLFIGDVGRPDLSGARGHTADTMAA
                     MLYESLHGKLLTLDDAVEVFPAHGAGSMCGRNISKETSSTIGQQRKFNYALAPMPEAD
                     FVRMMTEGMPPAPNYFSRDAEINRTGASALEALPRPQALTPEAVWEQVQRGALVLDVR
                     AAADYGAAHIPGSLNIGLGGQFASWAGSLISPESALIIVASDETKAEEAVLRLARVGL
                     ENVRGFLQGGMDAWLQAGQDAAAIPQITVAELHRRLEAEPDLQLLDVRRSGEYAEAHV
                     PTARLAPLAELQANTLDWLDPQRP"
BASE COUNT         1245 a         1929 c         2156 g         1269 t
ORIGIN      
        1 tatcgcacag agtccacaga cgctcagcag cgtcatgtat ggtttggcga aacgctgttt
       61 acctgcctgt tgcatcgtca tgcgggtttt gcgcgggaag ttgttttaga cgtgggtgcg
      121 aagttgagaa agcgaatgac gggcgcgctc taacctttca agcgcgcccg tcattttaat
      181 cggcagacgc gtcgtgcggc gcgcaaactt tacttcacgg tcatgcagga agtgttatcg
      241 ccattcgtca acgccagttt ggcctgcgcc cggaagtcac cgatgagaac gccgtcgccg
      301 atgagtacac cgtcgccaat caacacgccg tcaccgatga gcacgccatc gccgatcaag
      361 actccgtcgc cgatgaggac gcccgttccg aacaggaagg agccttcgcc aatcgtcgcg
      421 ccgtagctcg taagaatagt atcgccgatc aagacgccgt cgccgatgag cacgccgtcg
      481 ccgatgagga cgccgctcga ctccgataga cctgcgccga gcaatgtgcc ttgatcataa
      541 atcttctggt atcgcgtaac gagatttgag ccgctggcgt aagtgtggcc gaggatgagt
      601 ccttgcgacc actggaaggt tctgccggag atggtggttt gcggcgtggg gagcgcgcag
      661 ttgtagcaga gcagcggcgc gccgagcgga agcgcggcgt tggaaacgtc ggtgcgcatc
      721 gccttcgcca gtcgtatcgc gccttcgatg ttgagttcgc ccgcgccctg ctcgaaatga
      781 ttgaatccgg cgagcggctg cgcggtatac atcaagatcg ttttggcgag attgggcgtg
      841 agcgaagggt tggcctgaag caacagggct gcgccaccgg ccacgacggg agccgccatc
      901 gaagtgcccg aaagatacat ctgctccctt gtggcgatga aactgacgtt ggcgtcgagc
      961 gcaggattac cggcgacgag ataattgcct gccgactgtg cggcgatgag cttgttgccg
     1021 ggagcgacga ggtcgggctt caacaagttg tcgtagtgcc ggatgcgttt cgaatctgtc
     1081 cagaagctac gcgtcggacc acgcgaactg taggtggtca caaggtcgtc ggcgcgagag
     1141 tttgtgccga ggctgttggc cgcgccaatc gtgaagacgg atggttcgtt gccgggcgag
     1201 tgaatctggc cgtagatttt ccgtcccgcg ctatccgtcc cttcgttgcc cgccgccgcg
     1261 acgacgacga ttccggcatc caccagacga cgcgccgcgc ggcagagcgg atcatatttg
     1321 taagaatcta ccgccgccgt gcccaggctc aagttgacga ctttgatctt gtaagcggca
     1381 ttggcgcgat tggcatagac ccaatcgagc gcgccgagca ggctcgaagt cgtgccccgg
     1441 ccttgagcat tgagcacgcg tagattgatg atccgggaat tgtaagcgat gccgcgatat
     1501 gcgccttccg acacgaggcc gtttccggcg gccagcgcgg cgacgtgcgt gccgtggccg
     1561 taaggatcgt cggtgcggtt ttcgcccgtg aagtcctgtt gatagacgat gcgtgatttg
     1621 cttgcggcgt cgaggaaggc cgtgtggttc gggtcgatgc cggaatcgat gatgacaatg
     1681 ccggtattcg aaccgtcgag cgtcgtcccg gtgccgccca tgctgcgcgc ggcatccgcg
     1741 ccggacgtga gggtcacatg gccgagttgt ttgacttcac ggtcgaggga gatgtattcg
     1801 acatcggcgc gctcggccag tgcgtgcacg gcgggagcgg ggagagtcag cgcacgcgcg
     1861 ttgaagtggg agtacttgcg cgtcgtgttg ccgttatgat aggtgatgtc cgaatcgagc
     1921 gattgactcc acgcgccgct cggttgaatg atgactttga tctgctcgcc gtttctggcc
     1981 ttgccgatct tttcgcgtaa gtctccggcg actttatctt tcttttcccg cacggagttt
     2041 gcactggccg gggcggaagg gaagaaccag acgcacaggg tcgccagcag caggaaagcc
     2101 gcaagcttcg aggtctggga aaacttgttg gagcgtccgg tcaaattcat tggatgcgaa
     2161 cgagtagggc gagacaaagt ctcaggggtg gtaactgccg ggcatcttgc gacttcggcg
     2221 cataatgccg ctcgacgcat cgttgggcaa tgattgtgcc agagacggct atgcggacgg
     2281 ctcgccgaaa gtgggcggaa acgggcgttt tgagaaactg gaagcgaagg cgggaaagcc
     2341 tgtccggtca acctgagaga gttcggtcag tcattttggc agcagggcgg gagcgaggga
     2401 gagcgtgcgc gcgtgaaaga tgcgcgggcg agtagttcca ttttggccgg ggggagggct
     2461 tttgaaagac tttgaggtgt tgcccgaaga ggtggaaatt tatcgcgacc ggatgtggca
     2521 gcgcgaaccg gagagccgtg tggagaacgc gctcgaagcg gaaaagctga tcgagcgggc
     2581 ggggttttgc gcggccatga cggacgcgcg ccgaccgggc gcgtcgctct acgtgagcgt
     2641 gtgcgggcgc agagacgcgc acatgccgcg caacgtgcag aaagacccgg agagcagcca
     2701 gacgtggttg ctgaaagatg agatcatgcg gcgcggtcgt gtctactatg cgaagctggc
     2761 gaaggcgcgt gcgctgttcg ttgcgcccag actcgtgccg cacttcaacg ctctgtgggg
     2821 cgtggcgcgc agggaagaga gcgcgcggct gtctgcggag gcgcgggcgg tgttgaagat
     2881 tctgcgacgc gagtgggaga tggcgacgtg cgatctcagg acgacggcga agttgggcga
     2941 gcgtgcgcaa ctgacgcgcg ctctggatga gttgcagcgt acgctcaaag tgattccgtg
     3001 cgacgtgtta tacgagccgt ggttcaccta catctggacg ctggcggaag ggcgttttgc
     3061 ggcggagttg aaggtgaagg tgaagcgcga agtggctctg cgcgaagtgg cgcgagcctt
     3121 tctcggaggg gctggacgga cagtgcgcgg cgaactggcg aaggtcacag gactctcaag
     3181 acccgacgcg ggactcggca accgtgcgct cgtcgccgaa ggctacgccg tgcgtctctc
     3241 ggaaggcgtc tatcaactcg cggatttcga gcagcgtttc cgggagatga acaactaact
     3301 gccgcctgct ctcctgttct tctctctaga ctttagttgg tgccgccgcc ggggagttgc
     3361 ctgtcggcgc gtagttcctg ctccacctgt tcgcgcgctt cgcgttcgag gcgggcggtt
     3421 tcgagcgagt ggggaacttg cgctgccgcg agttcgaggc gttggagtgc gatgcgcgtc
     3481 tcttcgtcgc tggcgttgcg gcctgccgcc gcttcgatca tcaacagtcg cgcttccgcc
     3541 tgcgcacgcc gctcggtggc ctcggcttcg atgcgaccga ggcgttcgcg gtcgcccttc
     3601 aactcttctt ccagcgtcgt gcgttcggcg actcccagac gcgccgtgtt ttgaatccgg
     3661 cgctcgacgg agacggcttc gcgccgggca tttgcggcga cgcgcgcagc cgtgatgcgt
     3721 gtggcgagcg tttcgcggta agcgtcggcc agcagcaggt tgcgcgtctc gatctcgcgc
     3781 agcgtgcgtc gctcggcttt cgtcaactcg gctgtgaacc aagacaattc gtcggtcgtc
     3841 acgccgtcgg cggccagcct gttacgcaga cgcttctccc attgccggcg atagatgaag
     3901 agcaggacga ccgcgacgag gccgatgatc gaacccgcag ccagcgtcgt catggcgagc
     3961 agcatgaact cgcccgcttc cgcgccggac gcggcgttga aataacggaa caggaaatag
     4021 aggggcacgg gcagcgcgat gatcaggaag gcgagccagg gaaatgtttg caggaggaaa
     4081 tgcgtcgcgc gttctttcgc tgtcagacgg ttgccggtgc tgttgcgttt atcgctcatg
     4141 taaagtgttc ttcgcttcga gacaatcaag ctatataatg ccttgtcaaa gaggaaagcg
     4201 acaaggtaca caggagagaa gatgaaaaaa ggattgccgc tcagactgct cttgctgttc
     4261 gtgctggcct gcgccgccgt cgccgacgcc gagacgtacc gcgacgcgga cgggcagaag
     4321 ggcgacggca acggtgcgcg tcgcgcggaa actcctcgca gcgtgcgcgc ggtgacgatt
     4381 ccggtcacag tcaaattgcg tgatgagcga ccggcgagcg aggatttgcg tgtgctcgac
     4441 ctcttcgtca cagaagacgg cgaacgacaa gagattctct cgactcgcag cgcagccgac
     4501 cacgcgccgc tcgcgctgac cctgctcgtg caggacgacc tcgcctctcc catcggcttg
     4561 gagatcggcg gccttgcgaa tttcattcgc aacttgccgc aaggctcgca cgtgcaggtc
     4621 ggttatctgc gctccggctc gctccagtta agacaaaaat tcacgaccga tctcgaacgc
     4681 gccgcgaaat ctctgcgcat ccccatcggc tcggccagcg ccgcgcccta caatccttac
     4741 gtgcagattt acgattcgct caaacgctac ggcggcgtgc cgctcggaac gcgccgcgcc
     4801 gtgctcgtta tctccgacgg cctcgacctg tcgcgcggct ttgacagttc ttcgccgaca
     4861 cacagcattg acctcgaccg cgccatcagc gaagcgcagc gccgcagcgt ggcgatttat
     4921 ggaatttacg cgccgacggt ggggcagggc ggcagcacgg ctctcgtctc taacgcgcaa
     4981 ggctcgctct tgcgtctcac gcaggaaacc ggcggcaaag cattttttca aggcacgggc
     5041 gcggctgtca gctttgcacc ctttctgcgc gaactctccg accgcctcgc gcgccaattt
     5101 gctttaacct atctctcgac gcacccggac aaaggttttc atcgcatcaa gattgattcg
     5161 cccggcaccg aagccgaact cgcctatccg gtcggctaca cgcgaaaatg agtccggctc
     5221 gcgcgtcgtt cgcggcctca aacgctttcc cccgagttgt gaacagttcg ctcgcggcaa
     5281 tatgcgcgcc cgtatcaagg ccgcatctgc gccgcgtcaa aatcaagtca acgtaagttg
     5341 tcggaggagc cggtgtactt caaacaattc tatctcggtt gtctcgcaca tgcttcatac
     5401 ctgatcggct cggaaggcga agcggcggtc gttgatccgc agcgcgacat cgagcaatac
     5461 atcgcggaga gcgcggcgca aaacttacgg atcaagtacg tcgtcgagac gcacctgcat
     5521 gcagactttg tgagcgggca ctgcgaactc gccgcgcgca ccggagccga gatcgtcttc
     5581 agccgccacg cgcgcgccgc tttcccgcat cgcgccgtgc aggacggcga cgagatcgaa
     5641 ctcggtcgcg tcaaactgcg cttctgggag acacccggcc acacacccga aagcctctgt
     5701 gtgctcgtga tcgacacgga tgtctccgac gaaccgcaga aagtcttgac cggcgatacg
     5761 ctctttatcg gcgacgtagg ccgccccgat ctctccggcg cacgcggtca taccgccgac
     5821 acgatggccg ccatgctcta cgaaagtctg cacggcaaac tgctcacact cgacgatgcg
     5881 gtcgaagtct ttcccgcgca cggcgcaggc tcgatgtgcg gtcgcaacat ctcgaaggag
     5941 acttcgtcca ccatcggcca gcagcgtaag ttcaactacg cactggcacc gatgcccgaa
     6001 gccgactttg tacggatgat gaccgagggc atgccgcccg ctcccaacta tttttcacgc
     6061 gacgcagaga tcaatcgcac gggcgcaagc gcgctcgaag ccttgccgcg cccgcaagcg
     6121 ttgacgccgg aagccgtgtg ggaacaggtg caaaggggcg cgctcgtgtt ggacgtgcgc
     6181 gcggcggcgg attatggagc cgcgcatatt cccggctcgc tcaacatcgg actcggcggc
     6241 cagttcgcct cgtgggcagg gagtctgatc tcgccggagt ctgcgctgat catcgtcgcg
     6301 tctgatgaga cgaaagctga agaagcggtg ctcaggctcg caagggtcgg gctggaaaac
     6361 gtcagaggct tcctgcaggg cgggatggac gcatggctac aggccggaca ggacgccgcc
     6421 gcaattccgc agatcacggt tgccgaactg cacaggagac tcgaagccga gccggacttg
     6481 caacttctcg acgtgcgacg ctccggcgaa tatgcagagg cgcacgtccc tacggcgcgc
     6541 cttgcgccgc tcgcggagct acaggcgaac acgcttgatt ggctcgaccc gcaaagacc
//
LOCUS       DASWNS010000511         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_71062, whole genome shotgun sequence.
ACCESSION   DASWNS010000511 DASWNS010000000
VERSION     DASWNS010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71062"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..685)
                     /locus_tag="VGB76_17630"
     CDS             complement(104..685)
                     /locus_tag="VGB76_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100771.1"
                     /translation="MDLISISTAIVLLAILIAWYALLEYEERRQRRDLKRPEYAPEMG
                     GRRAHPERAQDARAYFTERLRRSDGSRLPEGMSLPTRSGQRLVFQGLLPRYGLAAASV
                     LLAYLIVTLGNLLFSFPPLIFFGAAVAFTFTVVGRAPGLFAFVLATLLSDFFFVKPTF
                     VFSLDWQVFRLSVFYLLGGLLSVFISKRWLWCK"
     gene            complement(805..1161)
                     /locus_tag="VGB76_17635"
     CDS             complement(805..1161)
                     /locus_tag="VGB76_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100772.1"
                     /translation="MVLLIGVLASLCFSVGEGLRLLPLPHLTHQPCAGADTRPQMTTR
                     NVSTLNQFQSGSITQPPQAQKNLKRKQSHWSSTSHCDLPLTNSLRLLSGAEWRAAFYL
                     TERASRQAGRAPPRLA"
     gene            complement(1310..>2385)
                     /locus_tag="VGB76_17640"
     CDS             complement(1310..>2385)
                     /locus_tag="VGB76_17640"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY0100773.1"
                     /translation="RVLSGSGIPYLITTLSPAGANEAEAEGLIVLRGDASHQRTLQLA
                     GVERAKMVVIADDDPATARRIAAVARTTNPTLRILVRTRYIAEIESFTEAGVDRVIAE
                     ELESIVGLFADVLRDYRIAAEEIDAHEEALRRGGYVALLRDPAEGESSVVECDLPEDC
                     LDTRTVTIRAGAPVTSEPLAAHALESKYGITLRSLRRDGDVVDDPPADFTLQAGDELV
                     LHATADSFARSAPLFRPQTGNLIDVTSAAAMLSNIDNVDGIDTDQTMELKPDPAASPA
                     CAHLGMARAVRPRARGCEECLQMGDTWVHLRVCMTCGHVGCCDSSKNRHASKHYPATD
                     HPIIKSLERGEDWAWCFADETYL"
BASE COUNT          478 a          711 c          739 g          457 t
ORIGIN      
        1 gaggacagta gccacacact agaacaggcg cggcggctcc caatgctgtt gcgcctgttc
       61 gcctttttgc gtgtctgacc tcaagcggtt ctgcccgcag atgctatttg caccagagcc
      121 atcgttttga gataaagacg ctgagcagcc ctcccagcag atagaagact gaaagcctga
      181 acacttgcca gtccagcgag aaaacgaaag tcggcttgac gaaaaagaag tcgctcagga
      241 gcgtggcgag gacaaaggca aagaggccgg gggcgcgccc cacgacagta aaagtgaagg
      301 ccaccgctgc gccgaagaat attaagggcg gaaacgagaa cagaagattg ccgagcgtca
      361 cgatcaagta cgcgagcagc acactggccg ccgcgaggcc gtaccggggg agcagccctt
      421 gaaacacgag ccgctgcccg ctgcgcgtag gcaacgacat cccctccggc aaccggctcc
      481 cgtcagaccg ccggagtcgt tctgtaaagt aggctcgcgc gtcctgcgct cgttcagggt
      541 gcgctctgcg cccgcccatc tccggcgcgt actccggtcg cttcaagtct ctgcgctgac
      601 gccgttcttc atactcaagc aaggcatacc aggcgattag aattgcgagc agaacaattg
      661 cggtcgagat ggaaatgaga tccatgatat ttcacccgtg cctgcatgca atacgggcgc
      721 aactctattg ccacgcacgt atgagcagcg gatatgacag acctgtttga atagaaaggg
      781 cggttgagat ttccggtcgg gtggttaggc taggcgcggg ggagcgcggc ctgcctgccg
      841 cgaggctcgc tcagtaaggt agaaggccgc acgccattct gcgccggata ggagtcgcag
      901 actattcgtg agtgggaggt cgcaatgtga agttgaagac cagtgcgact gtttacgctt
      961 gaggtttttc tgcgcctgcg gcggttgggt gatcgagcct gactgaaatt gattgagcgt
     1021 ggagacgttc cgcgtcgtca tctgcgggcg ggtgtcagca cccgcacacg gctggtgcgt
     1081 cagatgcggc aacggcagca ggcgcaaccc ttcacctacc gaaaagcaca ggctcgccaa
     1141 cacgccgatc aataaaacca tgcgtacact acgtgccttt actgccggag actctctacg
     1201 catctgattt ttaatcgtat caagctgctt cgctcgcggc aacctttgtc tctaagcctt
     1261 gcgttagaac cgcgacgcgt tggctccgtc acaatcgttt cggcgttccc tacaagtaag
     1321 tttcatcggc gaagcaccac gcccaatctt cgccccgttc gagcgatttg ataatcgggt
     1381 ggtcggtcgc cgggtagtgc tttgaggcgt gcctgttctt cgaagagtcg caacagccga
     1441 catgcccgca cgtcatgcag acgcgcaggt gaacccacgt gtcgcccatc tgtaagcact
     1501 cttcgcagcc gcgcgcgcgc ggcctgacgg cgcgagccat ccccagatgc gcgcaggcgg
     1561 gagaggccgc cgggtctggt ttcaactcca tagtttgatc ggtgtcgatg ccgtccacgt
     1621 tatctatgtt actgagcatt gcggcagccg aagtgacatc aataagattt cccgtctgag
     1681 ggcgaaacaa aggcgcactg cgtgcgaagg agtcggcagt ggcgtgcagc accagttcgt
     1741 cgcctgcttg cagggtgaag tcagcgggcg gatcgtcaac gacatcgcca tcacggcgca
     1801 agctgcggag tgtgatgccg tacttacttt caagcgcgtg cgccgcgagc ggctccgacg
     1861 taacgggtgc gcccgcgcga atcgtcaccg tgcgcgtgtc gaggcagtct tcgggtagat
     1921 cgcactcgac caccgacgac tcgccctctg ccgggtcgcg caggagcgcg acataaccgc
     1981 cgcgccgcaa cgcttcttcg tgcgcgtcga tctcttccgc cgcgatgcgg taatcgcgca
     2041 gcacgtctgc gaagagtccg acgattgact caagctcttc ggcgatcacg cggtcaactc
     2101 cggcctccgt gaatgattcg atttcggcaa tgtagcgtgt gcgcacgagg atgcgcagcg
     2161 tcgggttcgt ggtgcgcgcg acggcggcga tgcgccgcgc cgtcgccgga tcgtcgtcgg
     2221 cgatgaccac catcttggcg cgctcgacgc ctgcgagttg gagcgtgcgc tggtggctcg
     2281 cgtcgccgcg caggacaatc aagccttcgg cttccgcttc gttcgcgccc gccggactca
     2341 gcgtggtgat gagataagga atgcctgagc cgctgaggac gcgca
//
LOCUS       DASWNS010000512        21290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_19419, whole genome shotgun sequence.
ACCESSION   DASWNS010000512 DASWNS010000000
VERSION     DASWNS010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 21290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21290
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19419"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VGB76_17645"
     CDS             <1..397
                     /locus_tag="VGB76_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100774.1"
                     /translation="ISADAPSDETPEQKRERLMADIKAKAAARMAAKNNPGGAKTGGE
                     TSIATPPLSAQPPSPTSGTDSSASADAHRPAAEAQAATGQASAAPLSPAAATASPAAG
                     AETPADLAPDASTELTPGAPRDERAADEG"
     gene            complement(550..1557)
                     /locus_tag="VGB76_17650"
     CDS             complement(550..1557)
                     /locus_tag="VGB76_17650"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100775.1"
                     /translation="MIIEATAPTRVDLAGGTIDIWPLYLFHPKAATVNFAISLYAHCR
                     IETRDDRRIILESRDRGLSFETDLDGVAALVHEERLELISKLVHFFKPETGFHLIAES
                     QAPAGAGLGGSSALTIACIGALNRLVGNRYEEHKFIMIAANVETTVIRVPAGFQDYYG
                     AFYGGANCIHFRADGIEREELQIDAAALESRIVICYTGEPRLSGINNWEMFKSHIEGD
                     KVIFQLFEGIRDAAQAMRTALSANDWQAVSAIMSAAYPNRKRLAPNITTPHMERLVEK
                     AGRHGAEAAKVCGAGGGGCIAFLCTEGKRETVARALSEEPDVQVLDWRFAPEGLTIKE
                     S"
     gene            complement(1646..2395)
                     /locus_tag="VGB76_17655"
     CDS             complement(1646..2395)
                     /locus_tag="VGB76_17655"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY0100776.1"
                     /translation="MEQYFYELVGQYGLYAIFFLAMIEGDITLLLAGVLAHGAFFGEY
                     SFVSVLAAGTAGGVAGDQLAYAGGRGFRSSIRDRRFYRAARPRIERLTDTFGLLSIFL
                     SKYVYGLRTASCVFYGVAKMPYARFLPLSVASCFVWVLLLSGAGYFFHGIVTNFIGDF
                     KHLGKYLLVIVVAGVVAFYLAERYWLSPKVEEADPERVHEFEENVQEKLQELGEEIKE
                     HLPPPLARRSKEKETVKKAHAASSSGDAEGD"
     gene            complement(2520..2981)
                     /locus_tag="VGB76_17660"
     CDS             complement(2520..2981)
                     /locus_tag="VGB76_17660"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0100777.1"
                     /translation="MNIRSILLPTDFSRCAESALPYAADLARQMKARLLCLHVIETTV
                     PPVGYPAALAEPLPVADMGGQLEESATRELPKLGARQECAGLDVEELLVRGDAAGEIV
                     RVARERDIDLIVISSHGRTGWGRMLFGSTAESVVRHAHCPVLVVKPKESDK"
     gene            3197..4156
                     /locus_tag="VGB76_17665"
     CDS             3197..4156
                     /locus_tag="VGB76_17665"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0100778.1"
                     /translation="MSTLTSDSNYIGINVSRASLAAALVSHEGQVLARRDSALENGDL
                     PAQLTRLVAELRDTAPAPVGALGLGVPGLVNPETGRVVVSSDLPSVVRADLQTELTRT
                     TNLPITLVNDANAAAYGEYSAGAGRGSRNMFYVTIGTGIGGALILDGKLWYGDSGFAG
                     EFGHITIDREGIECTCGNTGCLETVASAPNIVRRTHERLIRDSTSSLTRLGLNKNFTA
                     ADIAHAANEGDDFAMLMIERTGRYIGTAVAAVINLLNIQRIVLGGGVMDAGKLILDPI
                     IREAGRRSFQPCFEATQIVAATLGADAVPTGAAMLARNAATTK"
     gene            4293..6092
                     /gene="lepA"
                     /locus_tag="VGB76_17670"
     CDS             4293..6092
                     /gene="lepA"
                     /locus_tag="VGB76_17670"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY0100779.1"
                     /translation="MNPERIRNFSIIAHIDHGKSTLADRLLELTGAVAGRDMEAQVLD
                     NMDLERERGITIKAHAVRLDYRADDGTDYVLNLIDTPGHVDFSYEVSRSLSACEGALL
                     VVDASQGVEAQTLANTYLAIENNLEFVPVINKIDLPGAEPDKAIEQIEQVIGLDTSNA
                     VLTSAKTGVGVHEVLEAIVRDVPYPKGDPDAPLKALIFDSWYDAYRGVIVLFRVIDGR
                     IKPGMKIRFFNTGRDYLVEAIGVNRPRPTPISELGVGEVGFLTASIKTVADVQIGDTI
                     TEAARPTTVPFPGFQEIKPMVFAGLYPVEANQYEELRDALDKLRLNDASFFYEPESST
                     ALGFGFRCGFLGLLHMEIVQERLEREFNLDLITTAPGVRYRVTTTDNEVLEIDSPAKM
                     PDPARIQQIEEPVILATILTSDEYLGGILPLLEEKRGVQRKFEYIAAKRVMLTYELPL
                     NEIVLDFYDRLKSVSRGYASLDYHFADYWTSDLVKLDILVAGEPVDALSLIVHRTNSE
                     SRGRALAEKMRQLIPRQLFEVPIQAAIGNKVIARETVKAMGKNVIAKCYGGDISRKRK
                     LLEKQKEGKKRMKRVGRVEIPQEAFLAVLKVNE"
     gene            6121..7764
                     /locus_tag="VGB76_17675"
     CDS             6121..7764
                     /locus_tag="VGB76_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100780.1"
                     /translation="MKRLLFVSLFCLASTISYAQHHATAPAGTGAVVLLPGLGEHHHP
                     TSTTNAEAQRFFDQGLILVYGFNHDEAVRSFRRAAELDPQMAMAHWGIALAVAPNYNS
                     TTMDEARRKTADEAIKKALALAAKAPEHERAYIEAMRRRLPGDAPADQKKLSLDYKEA
                     MAALVQRYPDDLEAATLYADSLMILNAWKLWSPDGRPATGTEEIVAVLESVLRRNPNH
                     IGANHLYIHAVEASPLPERALPSADRLGGLSPAAGHLVHMPAHIYQRLGDYEASARVN
                     DLAARADRAYIEANGAQGIYPAAYYSHNLHFLAAAYSMQGRFRDALAAAEGLEANVRP
                     RLSEMSFLEDFLPTKTQLLVRFRRWDDILKSPEPPASQTYTNALWHWARALAYAATGK
                     KESASAEQKIFAAAFKALPAGARFGANAGRDVLQIAEHVLAARLASARGERKLAVELL
                     RQAVAAEDALHYSEPPDWYYPPTRESLGGALFAGGEYTEAERVFRAELERNRRNGRAL
                     FGLSESLRAQGKTYAAALVRQEFERAWKNADTQLKIEDL"
     gene            8200..9438
                     /locus_tag="VGB76_17680"
     CDS             8200..9438
                     /locus_tag="VGB76_17680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0100781.1"
                     /translation="MSNHVRANSAVPPARTASARALRRVTLTFLAIEFLDELVDGSRQ
                     AAWPLIRRDLSLSYTEIGLLLTIPNLVASIVEPPLDLLGDTRHRRAIILGGGLSFALA
                     MLFVSLSTGFLFLLLAFVLFYPASGAFVSLSQAALMDSAPARREQNMERWAFAGSLGI
                     VAGSLAINATVSAGASWRVWFAAMSALSLLLVLAARRSIFDAPAASVAVAGSQDEHAG
                     IRQGARDALGALRRGEVRRWLVLLQFSDLMLDGFHGFLALYFVDVVGLTDARAGLAIA
                     VWTVVGLGGDFLLIPLLERVRGLSYLRWSAAAMLFLLPAFLLTPNVPAKLFLLGLIGI
                     TNAGWYSILKAQLYSSLPERSGAVLALDNVAGFIGGSVPFALGLFAEHFGLSRMMWLL
                     LAAPLALLFAIPRREKSFQT"
     gene            9590..11794
                     /locus_tag="VGB76_17685"
     CDS             9590..11794
                     /locus_tag="VGB76_17685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0100782.1"
                     /translation="MKNLTSYRFFTKTLVPRLLLALACLLATGQAAFAQETRPTPQTP
                     PQQNPADDVLRVSTTLVQTDVSIVDKQGQFVEGLQREQFELKVDGRPQPILFLERVTA
                     GGASEAARIAAARGETAAAVANSEAADYGRSVIFFVDDLHLTPSGIVRARQLLLNFID
                     REMTPGTQVLIASASGQLGFLQQFTSEREALRAAAGKLSYFPQAALDNERPIMSPYLA
                     MAIDRGDREATAYMMRQMLSTENAVRLRASRIRRQAALLSSATFTSLESVVRLRSPLP
                     GRQTLFYISEGFAFDSQEADINYKLQRVTDAAARTGTVIYTLDARGLSVGMESLDASS
                     GGSPDPELNGYNPMGELSASQEILRAVAADTGGRALLNTNALEAAVARTLAETANYYV
                     LAWRPEGALASTEGAPKFRKLNITVKGRPELTVRLRRGFLNAPTVSAPVAANAPVAGR
                     TVAATAGTAKAALNATLNAPVARRELLVDTYPLFANDEQAGSVVLAPVQLANDRLQFT
                     TVGDKQQASVDLACIVLDDKGKVVFSEGKTMTVTGGAANAANSATGGERGKFFTSFSV
                     PISAPGLYQFRVAAHDNLSGLVGSTYRWVEVPDLKAKRLSLSSLFLSEARRANAGGAA
                     DASQETLKADRRFARTSGLLLQFQIYNAARRADAGAPDAEVEVKVLDGNNRVVLNAPP
                     RKASVAGDPARIPYAAEIPLGKLTTGAYSLQVTVTDAIARTNATRRVDFTVR"
     gene            11863..14916
                     /locus_tag="VGB76_17690"
     CDS             11863..14916
                     /locus_tag="VGB76_17690"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEY0100783.1"
                     /translation="MHVTRVELDNIKSYERADFSFEPGTTAIVGPNGAGKTTILEAIA
                     WTLFDTLDYSKDDFLRRGAKKGSVRVTFESDVDGRRYTVYRDTANGYYIYDPELKLKL
                     EEKKANVAPMLGKLLGIEAGTDLPALFKSAIGVPQGLLTADFLKTSSQRKAAFDRLLK
                     VEEYREGAERLRDTVNLIRERVSETRERIGRAEGTLARYEEMTAEHKTTEARARELAD
                     ALAALQREVVERKGRVAAFEEGERRVNESLTRAERAEVERKAAERSRAEVQSKLDEAL
                     RARAQQQASAADYQAHLDALAELSRLEAERAERERLLGEMNRVANLLAAAESDVRRLT
                     LALEAAGRARESLAEIEREVSAQEELERERERLRELRSHALAAREQLARLDAELNNLR
                     AQHAQTREQLRQAERAQAAPARVAQLESEQLGIHNALSRAEKAATDRKHLTNQRRDLT
                     REIKRLQETVGALERKMRDLESRAAGAERAAELEASGRELAEQSAHLRAEIARDEKMR
                     AEVKGGLCPILSERCLNIGEGQTLEGYFKDHLTANRTQLQTVQAEATRVEKQVRTARE
                     AGVALVQLERERAQLAHERQLLGARESLLSTLDRELAALPSDDRALDELRSKLIGVDA
                     DLINAREALLRYAELEPLGARLKEIEEEGKRKKEARAELAANASAIESLERDIAETDR
                     RLRALNDPRARLNLKRAEAEQETTLQAEVAGARDATGALERERAGLEEQSQRFQNFAT
                     EWTATLARRDATAPAYRLYLESASLAATLGVREAEVSRATEESARAAREAETAQAEHA
                     EAVAAYDRAAHSSERDALALALASEANTTARLEHARETAERLAAEIARLEEVRARWQE
                     EIRAQDRLKRLDETTEFMRDVLKKAGPEVTRSYVAHISVEANQFFREITGDAGRTLRW
                     SSDYEIMLEENGHERSFVNLSGGEQMVAALSVRLALLKQLSDIRIAFFDEPTVNMDAE
                     RRENLARQIGQVRHFNQLFVISHDDTFEEAVDHVLVLTGEHEAAA"
     gene            14916..15392
                     /locus_tag="VGB76_17695"
     CDS             14916..15392
                     /locus_tag="VGB76_17695"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HEY0100784.1"
                     /translation="MSAPRLKEVTIYCDGSSLGNGQAETRAAAVALLNFRDRWRAFGC
                     YLGSATNQQAEIVAAAIGLEQLREPCRVRVLTDSRYVVETMAGRFRRKSNHEWWERLD
                     KAREKHRIEWEWIKGHAGHNAQEAVDKAARRIAALGRVDESVLREAAQTLNPSHGG"
     gene            15483..17468
                     /locus_tag="VGB76_17700"
     CDS             15483..17468
                     /locus_tag="VGB76_17700"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY0100785.1"
                     /translation="MSSSVHIVSPLRARVVLMLALCGVLAAGAATAQTRGWLWQNPLP
                     QGNAIHALRFAADKQHGWATGADGAILHTDDGGYEWEAQGAPVNTTLYGLFVKDKQTA
                     VAVGARGVVLTTENGGERWLVRPTNVKDHLYNITFAGDDARHGWACGTYGRIIATTDG
                     GRTWREQTSGVAAHLFSISFGDKQTGVAVGANGTMLTTTDGGANWKSQPQAKEFPLTG
                     VAFAGATRRAVAVGYGGLVLKTDDGGATWARVEVYQQADLLTVYFIDEQHGWAAGNDG
                     VVLTTANGGETWLPVSVKTQPRLTTIHFADTTTGWAAGGDGLMLRTDDGGLEWRRLSE
                     GGREDLADIFFLDGSRGWAVGSRGRILYTASGGKRWTVRPSGVTVRLTHVFFVNETHG
                     WAVGDAGTILRSTNGGITWAKSDVLITHELSGVHFADEKRGIAVGARGTILSTEDGGE
                     TWRQRRSDTITWLEDVSYAGERVAVAVGAQGMILRTEDGGETWRYVETNSKEWFYSVA
                     FADGRRGFIVGEGGLILRTDDAGLKWKQQESKVKLSLFAISVATPLDALIVGEQGRVM
                     LTKDGGENWDALASATSVPLFSVAYRGGSAAWVAGRGGAILRRTDAVATINIPRPKLP
                     PALRGKKPKLETDDEAPVNERDIPPALPRDKKRPPSS"
     gene            17628..18221
                     /locus_tag="VGB76_17705"
     CDS             17628..18221
                     /locus_tag="VGB76_17705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0100786.1"
                     /translation="MRFCVKCGWQLIQTAAGGNETVGLFPAKASSALGDDIHATAAPP
                     TSQLQQGEDAGTQEFTPQDFLVLVVDDTVDNLVIISLHLQQSGYRVVTATNGEEAIKV
                     AALTNPDLILMDLSMPGLDGLGATRKIREHPTLRPVPVVAVTAFNTEGFKRAAHDAGF
                     DGYITKPIDFERLNDLIRSLLPVKKPDDLDTLGGGDE"
     gene            complement(18297..18506)
                     /locus_tag="VGB76_17710"
     CDS             complement(18297..18506)
                     /locus_tag="VGB76_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100787.1"
                     /translation="MRDPFLAGLLSFVIPGLGQLYNGRILAGILWLILTPGFWLGTGG
                     MLGWVCHVVAAYTAYSYAKEHRVRI"
     gene            complement(18692..20797)
                     /gene="dnaX"
                     /locus_tag="VGB76_17715"
     CDS             complement(18692..20797)
                     /gene="dnaX"
                     /locus_tag="VGB76_17715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY0100788.1"
                     /translation="MAYQVIARKWRPQTFEEVTGQEPITRTLCGALEHERLHHAYLFS
                     GARGVGKTTTARLLAKALNCHKFQRPTATPCKTTDPDQCASCREVAEGRSIDVLEIDA
                     ASNTGVDNVRESIINTVGISPARDRYKVFIIDEVHMLSGAAFNALLKTLEEPPPRVVF
                     IMATTESHKIPDTILSRSQQFEFRTIATARIAERLRLIADAEKINVADEALREIARAG
                     EGSMRDAQSAFDQVISFSDAEINVADVEAALGIAGAELRTRIMRSIAESTPKDALAVV
                     DDLVMRGHDLRNFCRDLLSHVRDLLVLKVAGEAAEAVDASDAERRELARMAGEFSESD
                     LVRFFHSLTETERTLREGAAQPRYQLEIGLVKLIEMRRLAPLGVILERLNALEEALRT
                     GKAPHASSGAPAPPASGGSGSAPRGGSGGMGGSSSSSGGRGAPPASRASAPPASSYEA
                     KPQDASSPVPQAATPRANGAENFNPAAPKQRETPVSPNAPPVMPGATTTTASAASSAA
                     DTPPWEESPWDDPYSTTPPPLKLVPPPSPDANNRFAPATTTAARTTPTSQYTPGASSQ
                     ATGDAGGIIEQIKTGLEDRRKPFLAIALEGARKVSFEGEELYVEFAPESKHLRESLAK
                     PESVKLLREVCESVCGREMSVSIVVKDKSESAQLPTARDAEAEEQRALRELAENHPSV
                     QQVLRAFHAEIVDVRRVDK"
     gene            complement(20840..>21290)
                     /locus_tag="VGB76_17720"
     CDS             complement(20840..>21290)
                     /locus_tag="VGB76_17720"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0100789.1"
                     /translation="QNAHDARGNSFSFCEARMKIKIKRLNHVQLCVPPGAEERAREFY
                     GRLLGLREIEKPAPLRANGGLWFEIADIQLHIGVEGGQSKSKRHPAFEVEDIEGVRTY
                     LEANNVRTKDEIALAGIERFSFFDPFDNRIELLEKTNDRQEASTTAN"
BASE COUNT         4049 a         7008 c         6577 g         3656 t
ORIGIN      
        1 tatctccgcc gacgcgcctt ccgatgaaac gcccgaacag aaacgcgagc gtctgatggc
       61 cgatatcaaa gcgaaggccg ctgcgcgcat ggctgccaag aacaatccgg gcggcgcgaa
      121 aacgggcggc gagacatcca tcgccacgcc gcccctgtcc gcgcaaccgc cctcgcccac
      181 ctccggcacg gactcctccg cttccgcaga cgcgcaccgt cccgccgccg aagcgcaagc
      241 cgccacgggt caggcgtcag ccgcgccctt gagtccggct gccgccacgg cctcgcccgc
      301 cgccggagcc gagacgcccg ccgacctcgc gcccgacgct tcgaccgaac tcacccccgg
      361 cgcacccaga gacgagcgcg ccgccgacga aggataaacg accggacaac aatcttcgga
      421 cgaagcgacg accggataaa cagccatctc ttcaagcgac aaagagacag caagcgacaa
      481 ggaaagagcg cgcgaaagtg gcttcccaac tttcgcgcgc tctttcttta ccgtgtgaac
      541 cgtagttcct tagctctcct tgatcgtcaa accttccggt gcgaaccgcc agtcgagcac
      601 ctgcacgtcg ggttcttccg agagcgcgcg tgcgactgtc tcgcgctttc cttcggtgca
      661 gagaaacgcg atgcaaccgc cgccgcccgc gccgcagacc ttcgccgctt ccgcgccgtg
      721 gcgtcccgct ttttcgacga gccgttccat gtgcggcgtc gtgatgttgg gcgcgagccg
      781 cttgcggttc ggataggccg cgctcatgat cgcgctcacc gcttgccagt cgttggcaga
      841 gagtgcggtg cgcatggcct gcgcggcatc gcgtatgccc tcgaagagtt ggaagatcac
      901 tttatcgcct tcgatgtgcg acttgaacat ctcccagttg ttgattcctg agaggcgcgg
      961 ctcgcccgtg tagcagatga cgatgcggct ttcgagcgcg gcggcgtcga tctgtaactc
     1021 ttcgcgctcg atgccgtcgg cgcgaaagtg tatgcagttt gcgccgccgt agaacgcgcc
     1081 gtagtagtcc tgaaagcccg ccgggacgcg gatcacggtc gtctccacgt tggccgcaat
     1141 catgatgaac ttgtgttcct cgtaacgatt gcccacgagc cggttgagcg cgccgatgca
     1201 cgcaatcgtg agcgcgctcg acccgccgag tcccgcgccc gccggagcct gcgactccgc
     1261 gatgagatga aaccccgtct ccggtttgaa gaagtggacg agtttcgaga tgagttcgag
     1321 ccgttcctcg tggacgagcg cggcaacgcc gtcgaggtcg gtttcaaaag acaacccacg
     1381 gtcgcgcgat tcgagaatga tgcggcggtc gtcgcgcgtc tcgatgcggc aatgcgcgta
     1441 gagcgagatg gcgaaattga ccgtcgccgc cttcgggtga aacaggtaga gcggccaaat
     1501 atcaatcgtg cctccggcca gatcgacacg cgtgggagca gttgcttcga taatcataag
     1561 ttaagtgaca ggtgacaggt ggcaggtgac aggcaaagac aagttttaat cttgtcgcct
     1621 gttacccgtc acctgtcact atgtttcagt ctccttcggc gtcgccgctg ctgctggcgg
     1681 cgtgggcttt cttgacagtc tctttctctt tgctgcggcg cgcgagcggc ggcgggaggt
     1741 gttccttgat ctcctcgccc aactcctgga gcttctcctg cacgttctct tcaaactcgt
     1801 gcacgcgttc ggggtcggcc tcttccacct tgggcgagag ccagtaacgc tctgcgaggt
     1861 agaaggcgac cactccggcg acgacgatca cgagcaggta cttgccgagg tgcttgaagt
     1921 cgccgatgaa attcgtcacg atgccgtgga agaaatagcc cgcgccgctc aatagcagca
     1981 cccacacgaa acaactcgcc accgacaacg gcagaaagcg cgcgtaaggc atcttcgcca
     2041 cgccgtagaa gacgcaggat gccgtgcgca ggccgtagac gtatttcgag agaaagatcg
     2101 agagcagccc gaaggtgtcc gtcagccgtt cgatgcgcgg gcgcgcggcg cgataaaagc
     2161 gtcggtcgcg gatgctcgaa cgaaacccgc gcccccctgc ataggcgagt tggtcgcccg
     2221 ccacgccgcc cgccgtgccc gccgccagca ccgacacaaa actatattcg ccaaagaacg
     2281 ccccgtgcgc caacacgccc gccagcagca gcgtaatatc cccctcgatc atcgcgagga
     2341 agaagatcgc gtacaaccca tactgcccca ccagttcgta gaagtattgc tccataagag
     2401 aacagtgacg agtgacaagt gacgagttaa agacaagcag ggcgtcggtc gcaacctccc
     2461 gaacaacgag agaatattct atttacgatt catgccttca actcgtcact tgtcactcgt
     2521 cacttgtcac tttcctttgg tttgacgacg agcacggggc agtgtgcgtg gcgtacgacc
     2581 gactcggcgg tcgagccgaa gagcatgcgc ccccagccgg tgcgcccgtg cgatgaaatg
     2641 acgatcaaat ctatgtcgcg ctcgcgcgcc acgcgcacga tctcgcccgc cgcgtcgccg
     2701 cgcacgagga gttcttccac gtccagcccc gcacactcct gacgcgcgcc cagtttcggc
     2761 aactcgcgcg tcgcggactc ctccaactgc ccgcccatgt ctgcgaccgg cagcggctcg
     2821 gccaacgccg ccggataacc gaccggcggc accgtcgtct cgatcacgtg caggcacagc
     2881 agccgcgcct tcatctgccg cgccagatca gccgcgtacg gcaaagccga ctcggcgcaa
     2941 cggctgaaat cggtcggcag cagaatggaa cgaatgttca taaagactaa agtgatgagt
     3001 gacgagtgat gagtgatgag taataagtca aaggctttca ctcattactc atcactcatc
     3061 actcatcact tttccggctc gtcattctac acgcagagag gcttgtttga cagcccccta
     3121 cccctttgtt accattttcg ccgtttccta cctgaacgac tttttcgcca gttactcccg
     3181 tccggagatc aaagacatgt caactttaac atccgacagc aactatatcg gcattaatgt
     3241 ttcccgcgcg tcgctcgccg ccgcgctcgt ctcgcacgaa ggacaggtgc tcgcgcgccg
     3301 cgacagtgcg cttgagaacg gcgacctccc cgcccaactc acgcgcctcg tcgccgaact
     3361 gcgcgacact gcgcccgcac ctgtaggcgc gctcggactg ggcgtgcccg gcctcgtcaa
     3421 cccggagacg ggtcgcgtcg tcgtctcctc cgatctcccc tcggtcgtcc gcgccgattt
     3481 gcagaccgaa ctcacgcgca cgacgaatct ccccatcacg ctcgtcaacg atgcgaacgc
     3541 cgccgcctac ggcgagtaca gcgcgggcgc gggacgcggc agccgcaaca tgttctacgt
     3601 caccatcggc acaggcatcg gcggcgcgct catcctcgac ggcaaactct ggtatggcga
     3661 ttcgggcttc gcgggcgagt tcggccacat caccatcgac cgcgaaggca tcgagtgcac
     3721 ctgcggcaac acgggctgcc tcgaaaccgt cgcctccgcg cccaacatcg tccgccgcac
     3781 gcacgaacgg ctcatccgcg acagcacttc atccttgacg cgtctcggcc tcaacaaaaa
     3841 cttcaccgcc gcagacatcg cccacgccgc caacgaaggc gacgacttcg ccatgctcat
     3901 gatcgagcgc acgggtcgct acatcggcac cgccgtcgcc gccgtcatca acctgctgaa
     3961 catccagcgc atcgtcctcg gcggcggcgt catggacgcg ggcaagttga tcctcgaccc
     4021 catcatccgc gaagccggac gccgctcctt ccaaccctgc ttcgaagcca cacagatcgt
     4081 cgccgccacc ctcggcgcgg acgccgtccc caccggcgcg gccatgctcg cacgcaacgc
     4141 ggcgaccact aagtagctgt ttcaggcaac cggctttcca gacgggcgcg cgtgcggttt
     4201 cgcgcccgtt ttgttatgtt aagccgtcag ccattagctt tcagcttttg tttccgctaa
     4261 tcgctgacgg ctaattgcta acggcttaaa taatgaatcc agaacgcatc agaaattttt
     4321 cgatcatcgc gcatatcgat catggcaagt ccactctggc cgaccgcctg ttggagttga
     4381 cgggcgcggt cgcggggcgc gacatggaag cgcaggtgct cgacaacatg gacctggagc
     4441 gcgagcgcgg catcaccatc aaagcccacg ccgtcaggct cgattatcgc gccgacgacg
     4501 gcacggatta cgtcttgaac ttgatcgaca cgccggggca cgtggatttc agttacgaag
     4561 tgtcgcggag tttgtctgcg tgcgagggcg cgttgctggt ggttgacgcg tcgcagggcg
     4621 tcgaagcgca gacgctggcg aacacgtatc tggcgattga gaacaatctg gagttcgtgc
     4681 cggtcatcaa caagatcgac ctgccgggcg cggagcctga caaggccatc gagcagatcg
     4741 agcaggtgat cgggttggac acgtcgaacg ccgtcttgac gagcgcgaag acgggcgtgg
     4801 gcgtccacga agttctggaa gccatcgtgc gcgatgtgcc ctatcccaaa ggcgaccccg
     4861 acgcgccgct caaagcgctc atcttcgatt cgtggtacga cgcttatcgc ggcgtgatcg
     4921 tgctcttccg cgtcatcgac gggcgcatca agccgggcat gaagatacgt ttcttcaaca
     4981 cggggcgcga ctatctggtc gaagccattg gcgtgaaccg cccgcgaccg acgccgatta
     5041 gcgaactcgg cgtgggcgaa gtcggtttcc tgaccgcttc gatcaagacg gtggccgacg
     5101 tgcagatcgg cgacacgatc acggaagcag cgcgaccgac gaccgtgccc ttccccggtt
     5161 tccaggagat caagccgatg gtcttcgcgg gtctttatcc cgtcgaagcc aaccaatacg
     5221 aagaactgcg cgacgcgctc gacaagttgc gtttgaacga tgcctcgttc ttctacgagc
     5281 cggaatcttc caccgcgctc ggcttcggtt ttcgctgcgg cttcctcgga ctgctccaca
     5341 tggagatcgt gcaggagcgt ctggaacgcg agttcaacct cgatctcatc accaccgcgc
     5401 ccggcgtgcg ttatcgcgtg acgacgaccg acaacgaggt cttggagatc gactcgcccg
     5461 cgaagatgcc cgaccccgcg cgcatccagc agatcgaaga gccggtcatt ctcgccacga
     5521 tcctcacctc cgacgaatat ctcggcggca tccttccgct cctcgaagag aagcgcggcg
     5581 tgcagcgcaa gttcgagtac atcgcggcga agcgcgtgat gctgacgtat gagttgccgc
     5641 tgaacgagat cgtgctcgac ttctacgacc gcctcaagtc cgtctcacgc ggctacgctt
     5701 cactcgacta tcatttcgcg gattactgga cgagcgatct cgtcaaactc gacatcctcg
     5761 tcgcgggcga acccgtggac gccctctctc tcatcgtgca ccgcaccaac tccgagtcgc
     5821 gcggtcgcgc cctcgcggag aagatgcggc agttgatccc gcgccaactt ttcgaagtgc
     5881 cgattcaagc cgccatcggc aacaaagtga tcgcgcgcga aaccgtcaaa gcgatgggca
     5941 agaacgtgat cgccaagtgc tacggcggcg acatctcgcg caagcgcaaa ctgctggaga
     6001 aacagaaaga aggcaagaaa cgcatgaaac gcgtcgggcg cgtcgaaatc ccgcaggaag
     6061 ccttcctcgc cgtgttgaaa gtgaacgaat agaaccttcc tcgcaaaagg agtagttgag
     6121 atgaagcgtc tgctgtttgt ctcgctcttt tgtctcgcct ctacaatctc atacgcgcag
     6181 catcacgcga cggctccggc gggcacgggc gcggtcgtgc tcctgcccgg actcggcgag
     6241 caccatcacc cgacttcaac cacgaacgcg gaagcgcagc gcttcttcga tcagggactc
     6301 atcctcgtct atggcttcaa ccatgacgag gcggtgcgtt ctttccgtcg cgccgccgaa
     6361 ctcgatccgc aaatggcgat ggcgcattgg ggcatcgcgc tcgccgtcgc gcccaactac
     6421 aactcgacga cgatggacga ggcgcgtcgc aagacggctg acgaggcgat taaaaaagct
     6481 ctcgcgctcg cggcgaaagc gccggagcac gaacgcgcct atatcgaagc gatgcgcagg
     6541 cgtctccccg gcgacgcccc ggcagaccag aagaaactct cgctcgacta caaagaagcg
     6601 atggccgcgc tcgtccagcg ttacccggac gacctcgaag ccgccacgct ctacgcagac
     6661 agcctgatga tcttgaacgc gtggaaactc tggtcgccgg acggacgacc cgcgacgggg
     6721 acggaagaga tcgtcgccgt gctggaatcg gtcttgcggc gcaacccgaa tcacatcggg
     6781 gcaaaccatc tctacatcca cgccgtcgag gcgtcgccgc tgccggagcg tgcgctgccg
     6841 agcgcggaca ggctcggcgg gttgtcgccc gcggcgggtc atctcgtaca catgcccgcg
     6901 cacatctatc aacggctcgg cgactacgaa gcgtctgcgc gcgtcaacga tctggccgcg
     6961 cgtgccgacc gcgcatacat cgaagccaac ggcgcgcagg gcatctatcc ggcggcttac
     7021 tacagccaca acctgcattt cctcgccgcc gcctacagca tgcagggacg tttccgcgac
     7081 gcgctcgcag ccgcagaggg gcttgaagcg aacgtccgcc cgaggctttc ggagatgtcc
     7141 tttctggaag acttcctgcc cacgaagacg cagttgctcg tgcgcttccg tcgttgggac
     7201 gacatcctca aatcgccgga gccgcccgcc tctcaaactt acacgaacgc cctctggcat
     7261 tgggcgcgtg cgctcgcata cgccgcgacg ggcaagaagg agagcgcgtc ggcggaacaa
     7321 aaaatctttg ccgccgcctt caaagctctg cctgcgggtg cgcgcttcgg cgcaaacgcc
     7381 gggcgcgacg tgttgcagat cgccgaacac gtcctcgccg cgaggctggc gagcgcgcgc
     7441 ggcgaacgca aactcgccgt cgaactcttg cgtcaggccg tcgccgccga agacgcgctg
     7501 cactatagcg agccgcccga ttggtactac ccgccgacgc gcgagtcgct cggcggcgcg
     7561 ctcttcgcgg gcggcgaata cacggaggcg gagagggtct ttcgcgccga actcgaacgc
     7621 aatcgccgca acggtcgcgc gctcttcggg ctgtccgaaa gtttgcgtgc gcaggggaaa
     7681 acttacgccg ccgcgctcgt gcgtcaggaa ttcgagcgcg catggaagaa cgccgacacg
     7741 caactcaaga tcgaagacct ctgaaccgtt aacgaggaat gaaaagcagg agtcagaaaa
     7801 tagaatccag aagtcagaat acaggagtca gaataaaacg tgcgcgtctc tttatttctg
     7861 actcctgtat tctgttttct gtcttctcct tcatcgtttt tctctacgtc tctgcgttga
     7921 agtttttggt tccgtttgaa ttgcaatcag ccagcaaata agtagaatgg agactcgatc
     7981 aatagccgag tcggaagcga gttgaatggt atgaacatca cgctgacgtt ccctccctgg
     8041 taactttcta atcccgcaat cctaccttct cacgcgccgc cgcccgcgcg acggctcagg
     8101 tatgctcttg cctgcacgtg gattcctccg cccggaagaa cgcttttttt cgctcactac
     8161 cccgaagcgt tcacacttcc aaacgagagg aatccgtaaa tgtcgaatca tgtccgggcg
     8221 aactccgccg tgcctccggc acgcacggcc tcggcgcgtg cgttgcgtcg cgtcacttta
     8281 acttttctcg caatagaatt tctggacgaa ctcgttgacg gctcgcgtca ggccgcgtgg
     8341 ccgctcatcc gtcgcgacct ctcgctctcc tacacggaga tcgggctgct gctcacgatt
     8401 ccgaacctcg tggcgagcat cgtcgaacct ccgctcgatc tgctcggcga tacccgacac
     8461 cgccgcgcga tcatcctcgg cggcggcctc tctttcgcgc tcgccatgct cttcgtttcg
     8521 ctgagcacgg gtttcctctt tttgctgctg gctttcgtgc tcttctaccc ggcgtcgggc
     8581 gcgttcgtca gtttgtcgca ggccgcactg atggactccg cacccgcgcg gcgcgagcag
     8641 aacatggagc gttgggcttt cgccggatcg ctcggcatcg tcgccgggtc gctcgcgata
     8701 aacgccaccg tctctgcggg cgcgagttgg cgcgtctggt tcgcagcgat gtccgcgctc
     8761 tctctgttgc tcgtgctggc cgcgcgccgt tctattttcg acgcgcccgc cgcctccgtc
     8821 gccgtcgctg gctcacaaga tgaacatgcg ggcatccggc agggcgcacg cgacgcgctc
     8881 ggtgcgctgc gtcgcgggga ggtgcggcgt tggctcgtgc tgctgcaatt ctccgacctc
     8941 atgctcgacg gctttcacgg ttttctcgcg ctctacttcg tagacgtggt cggcctgacg
     9001 gacgcacgcg cagggctggc gattgccgtc tggaccgtag ttggactcgg cggcgacttc
     9061 ctgctcatac cgctgctgga acgcgtgcgc ggcctcagtt acctgcgttg gagcgcggcg
     9121 gcgatgctct ttctcctccc ggccttcctg ctcacgccga acgttccggc caaactcttc
     9181 ctgctcggat tgataggcat cacgaacgcc ggttggtatt cgatcctcaa ggcgcaactc
     9241 tattcgtcgc tgcccgaacg aagcggcgca gtgctcgcgc tcgacaacgt cgcgggcttc
     9301 atcggcggct ccgtcccgtt cgcgctcgga ctctttgcag agcattttgg cctgagccgg
     9361 atgatgtggc ttttgctcgc cgcgccgctc gctctgctct tcgccattcc gcgaagggaa
     9421 aaaagttttc agacatgata ggctaatgtt tcgacgcctg ccgccaattc gcgtccgaga
     9481 gatttcgggc gctttcggga gatccggcgc gacccggcga ccgcagccgt cgaattaaca
     9541 attaagggta gtgcaaccgg cgacgcaaat tgtcgcttcc aagtaagcca tgaaaaattt
     9601 aacctcgtac cgctttttca cgaaaacgct cgtcccgcgc ctcctgctcg cgctcgcctg
     9661 cctgctggcg acggggcagg ccgcgttcgc gcaggagacg aggccgacac cgcaaacgcc
     9721 gccgcagcag aatccggcgg acgatgtcct gcgcgtcagc acgacgctcg tgcagacgga
     9781 cgtgtccata gttgataagc aggggcagtt cgtagagggc ttgcagcgcg agcagttcga
     9841 gttgaaggtt gacggcaggc cgcagccgat tctctttctg gaacgcgtta cggcgggcgg
     9901 cgcgtccgag gcggcgcgca tcgcggcggc gcgcggcgag acggcggcgg cggtcgccaa
     9961 ctccgaggct gccgactacg ggcgcagcgt aatcttcttc gtggacgatc tgcacctgac
    10021 gccttcaggc atcgtgcgcg cacgccagtt gttgctcaac ttcatcgacc gcgagatgac
    10081 gccgggcacg caggtgttga tcgcgtcggc gagcgggcag ctcggatttt tgcaacagtt
    10141 cacgagcgag cgggaggcgt tgcgcgccgc cgccggaaaa ctttcatact tcccgcaggc
    10201 cgcgctcgac aacgagcggc caatcatgtc gccctatctg gcgatggcga ttgatcgcgg
    10261 cgaccgcgag gcgaccgcct acatgatgcg acagatgttg agcacggaaa acgcggtcag
    10321 gctgcgcgcg agccgcatcc ggcggcaggc cgcactgctc agcagcgcga cgttcacctc
    10381 tctggaaagc gtcgtccggc tgcgcagccc tctgcccgga cgacagacgc tcttctacat
    10441 ctccgagggc tttgccttcg acagccagga agccgacatc aactacaagc tccaacgcgt
    10501 gacggacgcc gccgcgcgca ccggcacggt tatctacacg ctcgacgcgc gcggcctgag
    10561 cgtcgggatg gagtcgctcg acgcgagcag cggcggcagc cccgacccgg agttgaacgg
    10621 ctacaatccg atgggagagt tgagcgcgtc gcaggaaatc ttgcgcgcgg tggccgccga
    10681 cacgggcggt cgcgccctgc tcaacacgaa cgcactggaa gcggcggtcg caaggacgct
    10741 ggccgagacg gcgaactact acgtgctcgc gtggcgaccc gaaggggcgt tggcctcaac
    10801 ggaaggcgcg ccgaaattcc gcaagctcaa tatcaccgtc aaaggccgac cggagttgac
    10861 cgtgcgcttg cggcgcggct tcctgaacgc gcccaccgtg agcgcgcccg tcgccgcaaa
    10921 cgcgcccgtc gccggtcgca cggtagcggc gacggcaggc acggcgaagg ccgccctcaa
    10981 cgcgacgctc aacgcgcccg tcgcgcgccg cgagcttctc gtagatactt acccgctctt
    11041 cgccaacgac gagcaggcgg gttcggtcgt gctcgctccc gtacaactcg ccaacgaccg
    11101 cctacagttc accaccgtag gcgacaagca gcaagcctca gtcgatctgg cctgcatcgt
    11161 gctcgacgac aaaggcaaag tcgtcttcag cgagggcaag acgatgaccg tgacgggggg
    11221 cgcggcgaat gctgcgaact ccgcgacggg cggcgagcgc ggtaaattct tcaccagctt
    11281 ttcagttccg atcagcgcgc cgggcttgta ccagtttcgc gtcgccgcgc acgacaacct
    11341 gagcgggctt gtcggcagca cgtaccgttg ggtcgaagtt ccagacctga aagcgaagcg
    11401 tctctcgctc agcagcctgt tcctgagcga ggcgcggaga gcgaacgcag gaggggcggc
    11461 agacgcatcg caggagacgc tcaaagccga ccggcgtttc gcgcgcacgt ccggcctgtt
    11521 gctgcaattc cagatctaca acgccgcacg cagggcggat gccggtgcgc ccgacgcaga
    11581 ggtggaagtc aaggtgctcg acggcaacaa ccgcgtcgtg ctgaacgcgc cgccgcgcaa
    11641 agcgtcggtc gcgggcgacc cggcgcgcat tccctacgcg gcggagatcc cgctcggcaa
    11701 gttgaccacc ggcgcatatt cgctccaggt cacggtcacg gacgcaatcg cgcgcacgaa
    11761 cgcgacgcgg cgcgttgatt tcacagtcag gtgaagccgc ttcgcgcccg gcgtccatcc
    11821 ggcgcgaatt ggcagaggca cgacgttaca ccgcaccaga tcatgcacgt cacacgagtc
    11881 gaactagaca acattaaatc ttacgagcgc gccgacttct ctttcgagcc gggcacgacc
    11941 gccatcgtcg gccccaacgg cgcgggcaag accacgatcc tcgaagccat cgcgtggact
    12001 ctgttcgaca cgctcgacta cagcaaggac gatttcctgc ggcgcggcgc gaagaagggt
    12061 tcggtgcgcg tcaccttcga gagcgacgtt gacggcaggc gttacaccgt ctaccgcgac
    12121 acggccaacg gctattacat ctacgacccc gaactcaaac tcaagctcga agagaagaag
    12181 gccaacgtcg cgccgatgct cggaaaactt ctcggcatcg aagccgggac ggacttgccc
    12241 gcgctcttca agtcggccat cggcgtgccg caaggcttgc tcacagccga ctttctcaaa
    12301 acgtcgagcc agcgcaaggc cgctttcgac cgcctgctca aagttgaaga gtaccgggag
    12361 ggcgcggaac gcctgcgcga cacggtgaac ctgatccgcg aacgcgtctc cgaaacgcgc
    12421 gagcgcatcg gtcgcgccga aggcacgctc gcgcgctacg aagagatgac ggcagaacac
    12481 aagacgaccg aggcgcgcgc gcgcgaactg gcggacgcgc tcgccgcctt gcagcgcgaa
    12541 gtcgtggagc gcaaagggcg cgtcgcggcg tttgaagagg gcgagcggcg cgtgaacgag
    12601 tcgctgacgc gcgcggaacg cgccgaggtc gaacgcaagg ccgccgaacg ctcgcgcgcc
    12661 gaagtccaga gcaaactcga cgaagccctc cgcgcacgcg cgcagcagca ggcttccgcc
    12721 gcagactatc aggcgcacct cgatgcgctc gcggaattgt cgcggctcga agcggaacgc
    12781 gcggagcgcg aacgcctgct cggcgagatg aaccgcgtgg cgaatctgct ggccgccgcc
    12841 gaatcggacg tgcgccgtct caccctcgcg ctcgaagcgg cggggcgtgc gcgcgaatcg
    12901 ctcgcggaga tcgagcgcga agtgagcgcg caggaagagt tggagcgcga gcgcgaacgc
    12961 ctgcgcgagt tgcgttcgca cgcgctcgcc gcacgcgagc aacttgcgcg tctcgacgcg
    13021 gaactcaaca acctgcgcgc gcagcacgcg cagacccgcg aacaactcag gcaggccgag
    13081 cgcgcgcagg ccgctcccgc gcgcgtcgcg caactggaga gcgaacaact gggaatccac
    13141 aacgcgctca gccgcgccga gaaggccgcg accgaccgca agcatctcac caaccagcgc
    13201 agggatttga cgcgcgagat caaacggctg caagagacgg tcggcgcgct cgaacgcaaa
    13261 atgcgcgacc ttgagagccg cgccgccgga gccgagcgcg ccgccgaact cgaagcgagc
    13321 gggcgcgaac tcgccgaaca gtcggcgcac ctgcgcgcag agatcgcgcg cgacgaaaag
    13381 atgcgagccg aagtgaaagg cggcctctgc ccgatcctct ccgagcgctg cctgaacatc
    13441 ggcgaaggcc agacgctcga aggctatttc aaagatcacc tgacggcgaa ccgcacgcag
    13501 ttgcagaccg tgcaggccga ggccacgcgc gtcgaaaagc aggtacgcac ggcgcgcgag
    13561 gcgggcgtcg cgctcgtcca gttggagcgg gaacgcgcgc aactcgcgca cgaacgccag
    13621 ttgctcggcg cgcgcgagtc gctgctctcg acgctcgacc gcgagttggc cgcgcttccc
    13681 tcagacgacc gcgcgcttga cgagttgcga agtaaattga tcggcgtcga tgcagacctc
    13741 atcaacgcgc gcgaggctct gctgcgctac gccgagcttg agccgcttgg cgcacggctc
    13801 aaagagatcg aagaggaggg caagcgcaag aaggaagccc gcgccgaact cgccgccaac
    13861 gccagcgcca tcgaatcgct cgaacgcgac atcgcggaga cggacagacg cctgcgcgcc
    13921 ttgaacgacc cgcgcgcacg cctcaacctg aaacgcgccg aagccgaaca ggagacgacg
    13981 ctgcaagcgg aagtcgcggg agcgcgcgac gcgacgggcg cgctcgaacg cgaacgcgcc
    14041 ggactcgaag agcagtcgca gcgttttcaa aacttcgcca ccgagtggac ggcgacgctc
    14101 gcgcgccgcg acgcaaccgc tccggcctat cgcctctatc tcgaaagcgc atcgctcgcg
    14161 gcgacgcttg gcgtgcgcga agcggaagtc agccgcgcca ccgaggagag cgcacgcgcg
    14221 gcgcgcgaag cggaaaccgc gcaggcagaa cacgctgagg ccgtcgccgc ttacgaccgc
    14281 gccgcgcatt cgtccgaacg cgacgcgctc gccctcgcgc tcgcctccga agcgaacacc
    14341 acggccagac tcgaacacgc gcgcgagacg gcggagagac tggccgcaga gatcgcgcgg
    14401 ctcgaagagg tgcgcgcccg ctggcaggag gagattcgcg cgcaggatcg cttgaagcgt
    14461 ctggacgaaa cgaccgagtt catgcgcgac gttctcaaaa aagccggacc cgaagtgacg
    14521 cgcagctacg tggcgcacat ctcggtcgag gcgaatcaat tcttccgcga gatcacgggc
    14581 gacgcaggca ggacgctccg ctggtcgagc gattacgaga tcatgctcga agagaacggc
    14641 cacgagcgtt cgttcgtcaa tctctccggc ggcgagcaga tggtcgccgc tctctccgtg
    14701 cgcctcgcgc tcctcaagca actctcggac atacgcatcg ccttcttcga cgagccgacg
    14761 gtgaacatgg acgccgagcg gcgcgaaaac ctcgcgcgcc agatcgggca ggtgcgccac
    14821 ttcaaccaac tcttcgtcat ctcccacgac gacacgtttg aggaagcggt tgatcatgtg
    14881 ctggtcctca cgggcgagca tgaggcggcg gcttgatgag cgcgccgcgt ttgaaggaag
    14941 tgacgatcta ctgcgacggg tcgagtctgg gcaacgggca agcggagaca cgcgcggcgg
    15001 cggtcgcgct cctgaacttt cgcgaccgct ggcgcgcctt cggttgctat ctcggttcgg
    15061 cgacgaacca gcaggccgag atcgtggccg ccgccatcgg actcgaacaa ctgcgcgagc
    15121 cgtgccgcgt gcgcgtgctc acggattcgc gttacgtggt cgagacgatg gccggacgct
    15181 ttcggcgcaa gtccaaccat gaatggtggg agcgtctgga caaggcgcgc gagaaacatc
    15241 gcatcgagtg ggaatggatc aaaggccacg ccggacacaa cgcgcaggag gctgtagaca
    15301 aagccgcgcg ccgcatcgcc gccctcggtc gggttgacga aagcgtgctg cgcgaggccg
    15361 cgcagacgct caacccatct catggcggct aaactggaaa cgcgcccgcg agaatgttaa
    15421 gtttgagccg gaatatttgt cgggtttgtg tctcaggatc attattctcc ccgcgttgat
    15481 tcatgtcgag ttccgtccac atcgtttcac ccctgcgcgc gcgcgtcgtt ctgatgctcg
    15541 cgctttgcgg cgtgctcgcg gcgggtgcgg cgacggcgca gacgcgcggt tggctgtggc
    15601 agaatccgct gccgcagggg aacgcgattc acgcgctgcg gttcgcggcg gataagcagc
    15661 acggctgggc gaccggcgca gacggcgcga tcctacacac ggacgacggc ggctacgagt
    15721 gggaggcgca aggcgcgccc gttaacacga cgctctacgg tctgttcgtt aaggataagc
    15781 agacggcggt ggccgtcggc gcacgcggcg tcgtgctcac gacggagaac ggcggcgagc
    15841 gttggctcgt gcgtcccacg aacgtcaaag atcatctcta caacataacc ttcgccggag
    15901 acgacgcgcg gcacggttgg gcttgcggaa cctacgggcg catcatcgcc acgaccgacg
    15961 gcgggcgcac ctggcgcgag caaacgtccg gcgtggcggc gcatctcttc tcgatctctt
    16021 tcggcgacaa acagacgggc gtggccgtcg gcgcgaacgg cacgatgctc acgacgacgg
    16081 acggcggcgc aaactggaaa agccagccgc aggcgaaaga gtttccgttg acgggcgtag
    16141 ccttcgcggg tgcgacgcgg cgcgcggtgg cggtcggcta cggcggtctg gtcttgaaga
    16201 cggacgacgg cggcgcgacg tgggcgcgcg tcgaagtcta tcaacaggcc gatctcttaa
    16261 ccgtctattt tatagacgag cagcacggtt gggcggcggg caacgacggc gtcgtgctca
    16321 cgacggcaaa cggcggcgag acgtggctgc ccgtcagcgt gaagacgcag ccgcgcctga
    16381 ccacgatcca cttcgccgat acgacgacag gatgggcggc gggcggcgac ggcctgatgc
    16441 tgcgcacgga cgacggcggc ctcgaatggc gcaggctctc ggaaggtggg cgcgaagacc
    16501 tcgcggacat cttcttcctc gacggttcgc gcggctgggc ggtcggttcg cgcgggcgga
    16561 ttctctacac ggcttcgggc ggcaagcgtt ggacggtgcg cccttccggc gtcacggtgc
    16621 gtctcacgca cgtcttcttc gtgaacgaga cgcacggctg ggcggttggc gacgcgggca
    16681 cgatcctgcg ttcgaccaac ggcggcatca cctgggcgaa gtccgacgtg ctcatcacac
    16741 acgaactgtc gggcgtccac ttcgccgacg aaaagcgcgg catcgcggtc ggcgcacgcg
    16801 gcacgatcct ctccacggaa gacggcggcg agacgtggcg gcagaggcgt tcggacacga
    16861 tcacttggct cgaagatgtc tcgtatgcgg gcgagcgcgt ggccgtggcc gtcggcgcgc
    16921 agggcatgat cctgcgcaca gaagacggcg gcgagacgtg gcgttacgtg gagacgaaca
    16981 gcaaggaatg gttctacagc gtcgccttcg cagatgggcg gcgcggcttc atcgtcggcg
    17041 agggcggttt gatcctgcgg acggacgatg ccgggttgaa gtggaagcaa caggagagca
    17101 aggtcaagct cagcctcttc gccatctcgg tcgccacgcc gctcgacgcg ctcatcgtgg
    17161 gcgaacaggg gcgcgtgatg ctgaccaaag acggcggcga gaattgggac gcgctcgcca
    17221 gcgcgacgag cgtgccgctc ttttcggtcg cgtatcgcgg cggctccgcc gcttgggtgg
    17281 cggggcgcgg cggcgcgatc ctgcgtcgca cggacgcggt cgccaccatc aacattcccc
    17341 gcccgaaact gccgcccgcg ctgcgcggca aaaaaccgaa gttggagaca gacgacgaag
    17401 cccccgtcaa cgaacgcgat atcccccccg ctctcccgcg cgacaaaaaa cgcccgccct
    17461 caagctgacg ttacgaggcg gaacgaagcg cggacgccgg actgtctcgc cattggcaaa
    17521 aacagcccca attctgcgat actgaaggta gacgaaaaac cgccggagag tggccgaaaa
    17581 aaacgatgat gtcctgtcca gtttgcgctc atgaaaatcc agacggcgtg cggttctgcg
    17641 tcaagtgcgg ctggcagttg atccagacgg cggctggcgg caacgaaacc gtcggactgt
    17701 ttccggcgaa agcgtccagc gctttagggg acgacataca cgcgaccgcc gcgccgccca
    17761 ccagccaact gcaacagggc gaggacgcgg gcacgcagga gttcaccccg caggactttc
    17821 tcgtcctcgt cgttgacgac acggttgaca acctcgtcat catcagcctg cacctgcaac
    17881 aatccggcta tcgcgtcgtc acggcgacga acggcgaaga ggcgatcaaa gtcgccgcgc
    17941 tcaccaaccc cgacctgatc ctgatggact tgagcatgcc cggcctcgac ggcctcggcg
    18001 cgacgcgaaa gatacgcgag cacccgacgc tgcgccccgt gcccgtcgtc gccgtcaccg
    18061 ccttcaacac cgaaggcttc aagcgcgccg cccacgacgc aggcttcgac ggctacatca
    18121 ccaaaccgat cgacttcgaa cgcctcaacg atctcatacg cagcctcctg cccgtgaaga
    18181 aacccgacga cctcgacacg ctcggcggcg gcgacgaatg aaaaagcgca ggccgcgcat
    18241 gtagaacgcg gcctgcgcca acttccattt tccccgacac ctttcggacg cacccgctag
    18301 atgcgcacgc gatgctcttt cgcgtagctg taagcggtgt acgcggcgac gacgtggcac
    18361 acccagccga gcatgccgcc cgtccccagc cagaagccgg gcgtcaggat caaccataaa
    18421 atgcccgcca gaatccgacc gttatacaac tgccccagtc cgggtatcac aaaactcaac
    18481 agacctgcca gaaaaggatc acgcatcgct atcaacctcc gtgcagacta tacgtgtgct
    18541 cccgcgcctt tgttcgcggg aaaaaagttg gctgccagtt gtcagccgca tgcgatcaac
    18601 tgtcagccat caaaccatct gccctcttca aacaccacag gccgcgcctc ttttgccggt
    18661 agctgaaggc tgacagctaa tcgctcacgg cttacttgtc cacgcggcgc acgtcaacga
    18721 tctcggcgtg aaaggcgcgc aggacttgtt gcaccgaagg atgattctcg gccaactcgc
    18781 gcagggcgcg ctgctcttcc gcttccgcat cgcgcgccgt cggcaactgc gcgctctcgc
    18841 tcttatcttt aacgacgatg ctcacgctca tctcgcgtcc gcacacgctc tcacacactt
    18901 cgcgcaaaag tttcacgctc tccggcttgg cgaggctctc gcgcaggtgc ttcgactccg
    18961 gcgcaaactc cacatacaac tcttcgccct cgaacgacac tttgcgcgca ccctcaagcg
    19021 cgatggcgag aaaaggcttg cgcctgtcct caagccccgt cttgatctgc tcgatgatgc
    19081 cgcccgcatc gcctgtcgcc tgtgaagatg cgcccggcgt gtattgactc gtgggcgtcg
    19141 tgcgtgcggc tgtcgtcgtc gctggtgcga acctgttgtt ggcgtccggc gacggcggag
    19201 gcacgagttt gagcggcgga ggcgtggtcg aatacggatc gtcccacggc gactcttccc
    19261 acggcggcgt gtccgccgca gaagatgctg ccgacgctgt tgttgtcgtc gctcccggca
    19321 tcaccggcgg ggcgttcggc gaaacgggag tctcacgttg tttcggcgcg gcggggttga
    19381 agttctccgc accgttcgcc cgtggcgtcg ccgcttgagg cacaggcgac gacgcatcct
    19441 gcggcttcgc ttcgtacgac gacgcgggcg gggcggaagc gcgcgaggcg ggaggcgcgc
    19501 ccctgccgcc gctactactg ctactgccgc ccatgccccc gcttcccccg cgcggcgcgc
    19561 tgccgctgcc gccgctcgcg ggcggtgcag gcgcacccga acttgcgtgg ggagctttgc
    19621 ccgtgcgcag agcctcttcg agcgcgttca agcgttcgag aatcacgccg agcggcgcga
    19681 gacggcgcat ctcgatcaac ttgaccagac cgatttcgag ttgataacgc ggttgcgccg
    19741 cgccctcccg gagcgtgcgt tccgtctccg tcaaactgtg aaagaagcgc acgagatcag
    19801 actccgagaa ttcgcccgcc atgcgcgcca gttccctgcg ttccgcgtcg ctcgcgtcca
    19861 cggcttccgc cgcttcgccc gccactttca agaccagcag atcgcgcaca tgcgataaca
    19921 ggtcgcggca aaagtttctc aagtcgtggc cgcgcatcac gagatcgtca accactgcga
    19981 gcgcgtcttt cggcgtactt tcggcaatcg agcgcatgat gcgcgtgcgc aattccgccc
    20041 cggcgatgcc gagcgcggct tccacgtccg caacgttgat ctccgcgtcg ctgaaactga
    20101 tgacctgatc gaaagccgac tgcgcatcgc gcatcgagcc ttcgcccgca cgcgcgatct
    20161 cgcgcagggc ttcgtccgcg acgttgatct tctcggcgtc ggcgatgagc cgcagccgtt
    20221 cggcgatgcg cgccgtggcg atggtgcgaa actcgaactg ctggctgcgc gagaggatcg
    20281 tgtcggggat tttgtgcgac tcggtggttg ccatgatgaa gacgacgcgc ggcggcggtt
    20341 cttccagcgt cttcaggagc gcgttgaagg ccgcgcccga aagcatgtga acttcgtcga
    20401 tgatgaagac tttataacgg tcgcgcgcag ggctgatgcc gaccgtattg atgatcgatt
    20461 cgcgcacgtt atcgacgccc gtgttcgagg ccgcgtcgat ttcgagcacg tcaatcgaac
    20521 gcccttcggc cacctcgcgg cacgaagcgc actgatccgg gtcggtcgtc ttgcaagggg
    20581 tcgccgtcgg gcgttggaac ttatggcagt tgagggcttt ggcgagcagg cgtgccgtcg
    20641 tcgtcttccc cacgccgcgt gcgccggaga agagataagc gtgatggagg cgttcgtgtt
    20701 cgagcgcgcc gcagagcgtc cgcgtgatcg gctcctgccc ggtcacttcc tcgaaagttt
    20761 gcggacgcca tttgcgtgcg atgacttgat aagccataaa gctgtgggtc gttcctggtc
    20821 agtaatccgt tgtcagtggt caattcgcgg tagtagacgc ttcttggcgg tcgttcgttt
    20881 tttctaaaag ctcgatgcgg ttgtcgaagg ggtcgaagaa ggagaagcgt tcgatccctg
    20941 cgagcgcgat ttcgtctttc gtccgcacgt tgtttgcttc cagataagtt ctgacgccct
    21001 cgatgtcttc gacttcaaag gcgggatggc gtttcgactt gctctggccg ccttcgacgc
    21061 cgatgtgcaa ttgaatgtct gcgatctcga accagaggcc gccgtttgcg cgcaggggcg
    21121 cgggcttctc gatctcgcgc aggccgagca gcctgccgta gaactcgcgc gcccgctctt
    21181 ccgcaccggg cggcacgcaa agctgcacgt ggttgagtcg cttgatcttg atcttcatgc
    21241 gggcttcaca aaatgaaaaa gagttgccgc gcgcgtcgtg tgcgttctgg
//
LOCUS       DASWNS010000513         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_329965, whole genome shotgun sequence.
ACCESSION   DASWNS010000513 DASWNS010000000
VERSION     DASWNS010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329965"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VGB76_17725"
     CDS             <1..320
                     /locus_tag="VGB76_17725"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100790.1"
                     /translation="ESVRIYPLAEWEGIEQRLALLPSMDPARRKFLDRTNYYGQQTAI
                     DGQGRVLIHPLLRKSAGVMGDVAVLGYLNYLEVWELERFQQRLLSDPYTEEDEAAIAR
                     LGI"
     gene            457..1431
                     /gene="rsmH"
                     /locus_tag="VGB76_17730"
     CDS             457..1431
                     /gene="rsmH"
                     /locus_tag="VGB76_17730"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY0100791.1"
                     /translation="MFASGGGPDAPHRPVLLAESIRHLAPERGGLFIDATLGLGGHSE
                     ALLESSPGVRVIGIDRDTEALRFARARLARFDQRFRAVHANFEEIAGIVGEAGGEELS
                     GVLADLGVSSLQFDSSERGFSFRHDAPLDMRMNAADDEEETAAELLARLPEEEIARII
                     FEYGEERKSRRIARWIVERREAGRAIRTTTELAELVARAVGYKRTDRIHPATRTFQAL
                     RIAVNRELEGLEAFLEASIDLLQSSGRLVVISFHSLEDRIVKRALRRLSGQCECDPRL
                     PVCACGARRTVEILTRRPITPTEAELEENPRARSAKLRACRKLEAVAS"
BASE COUNT          341 a          483 c          522 g          322 t
ORIGIN      
        1 gtgagagcgt gcgcatctac cctctggccg aatgggaagg catcgaacag cggctcgcgc
       61 tgctgccctc gatggacccg gcgcggcgca agtttctgga tcgcaccaac tattacggcc
      121 agcagacggc catcgacgga caggggcgcg tgctcattca tccactcctc agaaaaagcg
      181 cgggcgtgat gggcgacgtg gcggtgctcg gttatttaaa ttatctggaa gtctgggaat
      241 tggaacgatt tcaacaacgc ctgctctcag acccctacac ggaagaggac gaagccgcca
      301 ttgcgcgact cggaatttga tggaaagtga cgggtgacaa gtgacaagtg acaagttcaa
      361 aaccaaaact tgcccggcca actctctctc gtcacctgtc aatcgttact tgtcactgtt
      421 tttcaggtct ttgaacgact tcacggggcg gtggtgatgt ttgcatcagg tggggggcct
      481 gatgcgcccc atcgccccgt gttgctagca gagtcgatcc ggcatctcgc gcccgagcgc
      541 ggcgggttat tcatcgacgc gacgctcgga ctgggaggtc atagcgaagc gttgctggaa
      601 tcctcgcctg gcgtgcgcgt catcggaatt gacagggaca ctgaagcctt gcggttcgcc
      661 cgcgcgcgcc ttgcgcgttt cgaccaacgc ttccgcgccg tgcatgcgaa cttcgaggag
      721 atcgcgggca tcgtcgggga ggcgggcggg gaggagttga gcggggtgct ggcagacctc
      781 ggcgtctctt cgctccagtt cgattcgagc gagcgggggt tcagttttcg tcacgacgcg
      841 ccgctcgaca tgcgcatgaa cgctgccgac gacgaagagg agacggcggc tgaactgctg
      901 gcgcggctgc cggaagaaga gatcgcgcgc atcatctttg aatacggcga ggagcgtaaa
      961 tcgcggcgca tcgctcgctg gatcgtcgaa cggcgcgaag cggggcgtgc gattcggacg
     1021 acgacggaac tggcggaact cgtcgcgcgc gccgtcggct acaagcgcac ggatcgcatc
     1081 catccggcga cgcgcacgtt tcaggcgttg cgcatcgccg tcaatcgcga actcgaaggg
     1141 ctggaagcat tcctcgaagc ctcaattgat cttttacaat cgtctggccg tctggtcgtc
     1201 atcagttttc actcgctcga agatcgaatt gtgaagcgcg ctctgcgccg tctgtcaggg
     1261 caatgcgaat gtgatccgcg ccttccggtt tgcgcctgcg gggcgcgccg caccgtcgag
     1321 atattaacgc gacgacccat cacccccacc gaagcggaac ttgaagaaaa cccgcgcgca
     1381 cgcagcgcaa aactccgcgc ctgccggaaa ctcgaagcgg tcgcaagtta agaagttgcg
     1441 acttgaaaaa ttaaatgact gacgcgctcg cgcttcagtt ccacgggtct gcaacagtca
     1501 gtacctattt tgttcggaga cggaaacagc gatgagaaga ttcccccaac aaaatttaaa
     1561 caacggcgtg cgacgcgaac tcgatgcccg cgcgctcaag cggcaactga caatgctcgt
     1621 ctgtggtctg gtgatggccg gaggttttgt cgccgccgcc gggcagca
//
LOCUS       DASWNS010000514         3321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_149999, whole genome shotgun sequence.
ACCESSION   DASWNS010000514 DASWNS010000000
VERSION     DASWNS010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149999"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VGB76_17735"
     CDS             complement(<1..1122)
                     /locus_tag="VGB76_17735"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY0100792.1"
                     /translation="MKAKSYLSLILSFICAINAAAQTVGTPAAVAAVPQHNLMPVPAS
                     VRFNAGRLPVSTTFNVAARGQTDARLLSGIDRFLRRIEGRTVLELPRGLSTDANAATL
                     VVEAGAPGKPVPSVEENESYTLEVAERQAVLKAPTTIGVLRGLETLLQLLDSDRDGYF
                     IPAVRIDDRPRFPWRGLLLDVARHFQPIEVLKRNIDAMAAVKLNVLHWHLTEDQGFRI
                     ESKKFPRLHTLGSDGQFYTQEQARDIVAYARERGIRVVPEFDLPGHATAWLVGHPELG
                     SAPGPYTIERRAGIFEPALDPTREEVYKFLDTFFGEMAALFPDAYMHIGGDENEGKQW
                     DRNPQIQAFMKEKGIKDNHALQTYFNQRVFTILKKHGKMM"
     gene            1298..2644
                     /gene="glmM"
                     /locus_tag="VGB76_17740"
     CDS             1298..2644
                     /gene="glmM"
                     /locus_tag="VGB76_17740"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY0100793.1"
                     /translation="MNSLKIGISGVRGVVGETFTPELAVAFAQAFGTYLDSGRILVCR
                     DTRTSGAMVHAAVLSGLLAAGCEVVDLDVCPTPSLQLAVSWLEAEGGISITAGHNPEP
                     WNALKFIRADGLYLNATQADELLDIYHQAEFAKATWRDIRTRVEREDALAHHLDTLTR
                     AFDVEMIRARRLTVAVDCCNSSCSLLSPRWLAALGCDVLAVNDDPTAPFPHAPEPGRQ
                     TMAQLRAVTLAGRADVGFAHDADGERLGLVMETGEMLSEEATLCLAAEIQLRREVGAV
                     VTNISTSACVDRVAARHGASVVRTPVGQAYVSEAMIETGAVVGGEGSGGVVVPRVQWT
                     HDSAAAVGLILEHLAATKEPLSALAAGLPRFQMLKQNMAVEPDRIHSFLQRLRDELER
                     EKIAYDETDGIKVSWPDGWAHVRVSNTESMIRTIAEAETAARARELLDWARDRLAR"
     gene            2783..>3321
                     /locus_tag="VGB76_17745"
     CDS             2783..>3321
                     /locus_tag="VGB76_17745"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0100794.1"
                     /translation="MIKTLLFALILVPVAFVSVRLPAAREVKTVKVVEVPGYTEGVVI
                     DRDGAIYISDVYNGTIYRIGADGTARSWAQTGAPNGHKILPDGTHLVCDGKERAVLRL
                     DSAGKIMGKASSESEGKPLRAPNDLTLDPRGGFYFTDPGGSNVENPIGTVHYVDTRGQ
                     THLVAGGLAFPYGIALRPDG"
BASE COUNT          599 a         1012 c         1085 g          625 t
ORIGIN      
        1 catcatcttt ccgtgctttt taaggatcgt gaacacgcgt tgattgaagt atgtctggag
       61 cgcgtggttg tccttgatgc ctttctcttt catgaaggct tgaatctgcg ggttgcgatc
      121 ccattgcttg ccttcgttct cgtcgccgcc gatgtgcatg taggcatcgg ggaagagcgc
      181 ggccatctcg ccgaagaagg tgtcgaggaa cttgtagacc tcctcgcgcg tcgggtcgag
      241 cgcaggctcg aagattccag ccctgcgttc aatcgtgtat ggacccggcg cgctgccgag
      301 ttcgggatga ccgacgagcc atgcggtggc gtgaccggga agatcgaact ccgggacgac
      361 gcggatgcca cgctcgcgcg cataagcgac gatgtcgcgc gcctgctcct gcgtgtaaaa
      421 ctgcccgtcg gagccgagcg tgtggaggcg tggaaatttc ttgctctcga tgcggaagcc
      481 ctgatcttcc gtcaggtgcc agtggaggac gtttaacttg acggcggcca tcgcgtcgat
      541 gttgcgcttc aggacttcga tgggttggaa gtggcgcgcg acatcgagca acaacccgcg
      601 ccaggggaag cgcgggcggt cgtcgatgcg aactgcgggg atgaagtagc cgtcgcggtc
      661 gctgtcgagc agttgcagga gcgtctccag gccgcgcagg acgccaatcg tcgtcggggc
      721 tttgagcacg gcctgccgtt ctgcgacttc gagcgtgtat gattcgttct cctcgaccga
      781 aggcacgggt ttgccgggcg cgcccgcttc gacgacgagc gtggccgcgt tagcgtccgt
      841 gcttaatccg cgcgggagtt ccaacacggt gcgcccttca atgcgtctga ggaaacggtc
      901 tatgccggac aggaggcgcg catccgtctg ccctctcgcg gcgacgttga aagtcgtgct
      961 gacgggcaga cgccccgcgt tgaatcgcac ggaggcgggc acgggcatca agttgtgttg
     1021 cgggacggcg gcgacggcag caggcgtgcc gaccgtttgt gcggcggcat tgatggcgca
     1081 gatgaaggag agaataagcg agagatacga tttagctttc atgtgttcct cgataaacag
     1141 gataagcgtg tctgtggcct aaaatagcat tggcggagtt atgtagtttc tcccatgaat
     1201 ctgccctgac ggcgtaagtt acggtgtcat acttgaggtt acggaattaa gacgggtaaa
     1261 agctactgta catgatgttt tgtgtgaaga gacaattgtg aactcgctca agatcggcat
     1321 cagcggagtg cgcggcgtgg tgggggaaac cttcacgccg gaactggcgg tagcgttcgc
     1381 gcaagccttc ggcacttatc tcgactccgg gcgcatactc gtctgtcgcg acacgcgcac
     1441 ctccggggcg atggttcacg cggcggtctt gtcggggctg ctcgcggcgg ggtgtgaagt
     1501 ggtcgatctc gacgtgtgcc cgacgccgag cctgcaactc gccgtctcgt ggctcgaagc
     1561 agagggcggc atctcgatca ctgcgggaca taaccctgag ccttggaacg cgctcaagtt
     1621 catccgcgcg gacggcctct acctgaacgc cacgcaagcc gacgaacttc tggacatcta
     1681 ccatcaggcc gagtttgcca aagcgacgtg gcgcgacatc cggacgcgcg tggagcgcga
     1741 agatgcactc gcacatcacc tcgacacgct cacacgcgcc ttcgacgtgg agatgatccg
     1801 cgcgcggcgg ctgacggtcg ccgtcgattg ctgcaacagt tcatgctctc tgctctcgcc
     1861 gcgctggctc gccgcgctcg gctgcgacgt gctcgccgtc aacgacgatc cgaccgcgcc
     1921 ctttccgcac gcgcccgaac cgggacggca gacgatggca caactccgcg ccgtcacgct
     1981 cgcagggcgt gccgacgtag gcttcgcgca cgatgccgac ggcgaacggc tcggcctcgt
     2041 catggagacg ggcgagatgt tgtcggagga agcgaccctg tgcctcgcgg cggagattca
     2101 actcaggcgg gaggttggcg cagtcgtgac gaacatctct acgagcgcgt gcgtggatcg
     2161 cgtcgccgcg cggcacggcg cgagcgtcgt gcgcacgccc gtcgggcagg cttacgtctc
     2221 cgaggcgatg atcgaaacgg gcgcagtcgt cggcggcgaa gggagcggcg gcgtggtcgt
     2281 gccgcgcgtg cagtggacgc acgacagcgc ggcggcggtc ggcctcatcc tcgaacatct
     2341 ggcggcgaca aaagaaccgc tctccgcttt ggccgccggg ttgccgcgct tccagatgct
     2401 caagcagaac atggcggtcg aacccgaccg catccactcg ttcctgcaac gtctccgcga
     2461 cgaactggag cgcgagaaga tcgcttatga cgaaacggac ggcatcaagg tttcgtggcc
     2521 tgacgggtgg gcgcacgttc gcgtctcgaa caccgaatcc atgatccgca ccatcgccga
     2581 ggccgaaacc gccgcacgcg cacgcgaact gctcgattgg gcgcgcgacc gccttgcccg
     2641 gtaatgctta actcgctgtg cacgcttcat gtcctccgtg tccgcgctgg tgaatgcccg
     2701 atcaattaag attcactgcc gggatacaga gggcacggag gggcacagcg aaaaccgccg
     2761 gactattgga gtaacccata ctgtgattaa aactcttctt tttgcgctca tccttgttcc
     2821 cgtcgcgttt gtgagcgtgc gtctccctgc ggcgcgtgag gtcaagacgg tcaaggttgt
     2881 ggaagtgccc ggctacacgg agggcgtcgt catagaccgc gacggcgcga tctacatctc
     2941 cgacgtttac aacggaacca tctaccgcat cggcgcggac ggcacagcgc gctcttgggc
     3001 gcagaccggc gcgcccaacg gccacaagat tctgcccgac ggcacgcatc tcgtctgcga
     3061 cggcaaggaa cgcgccgtgc tgcgtctcga ttcggcgggc aagatcatgg gcaaggcatc
     3121 ctccgaatcc gagggcaaac ccttgcgcgc gccgaacgat ctcacgctcg acccgcgcgg
     3181 cggtttctat ttcacagacc ccggcggctc gaacgtcgag aaccccatcg gcaccgtcca
     3241 ctacgtagac acgcgcggcc agacacatct cgtcgccggg gggctggcat ttccgtacgg
     3301 catcgcgctg cgccccgacg g
//
LOCUS       DASWNS010000515         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_459967, whole genome shotgun sequence.
ACCESSION   DASWNS010000515 DASWNS010000000
VERSION     DASWNS010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459967"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VGB76_17750"
     CDS             complement(<1..589)
                     /locus_tag="VGB76_17750"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0100795.1"
                     /translation="MYDVIIIGAGAAGLSAALWCEELGLDALVLEAGSEIGGQLLSVY
                     NPVNNYMGVRAGNGRELRDLFAAQVEDCEFDLWTETEIESVDLRARRVQLRSGEALQA
                     ISLMIATGVRRRRLGIPGEAELAGRGVVESATRDRELLAGRDVCVVGGGDAAAENALL
                     LAEVCPTVTLVHRGRRLSARAEFVERLRSEHCITVF"
     gene            complement(681..1745)
                     /locus_tag="VGB76_17755"
     CDS             complement(681..1745)
                     /locus_tag="VGB76_17755"
                     /inference="COORDINATES: protein motif:HMM:NF042562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6687 family protein"
                     /protein_id="HEY0100796.1"
                     /translation="MKGFARMRFEYYHAGLDGVPKLSVDGLVPESVHFSHWQGNRTPA
                     QVKADSSTEIALNVVASPDRHELTRGIELVTNNHFDTDGVLSVWTMLTGERALDLRDA
                     LIAAAEAGDFSANTGAQAIRASLVLQGGDGFAPDAGVASPLARHLAGDASVDEERAYA
                     LVLPEVERVITRTNYYEHLWHDEWAKIEASLESFARGASRVEEDPETGLSVVTLSLEL
                     YGAQGFKPTRHAAPYTAITEHARGRIYLIAIPLERAWGYRVDYPYYSWAETTVRPAVS
                     RYDFAPLLARLGELEQNAEGRWQVDTEGLSSAFNFAGADNLLRASSLAPEQVADELRA
                     ALREQAEQRSVAVDRLSVVS"
     gene            complement(1875..2213)
                     /locus_tag="VGB76_17760"
     CDS             complement(1875..2213)
                     /locus_tag="VGB76_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100797.1"
                     /translation="MIDSDLFEPYERLITIEILGRRVEVPENNRLLRCFQFLSMRSIS
                     YGDFCWNGDCTNCQFWYRDEGEPARDDKTALSCRFKVREGLSITRLNEHVKLVGINKA
                     KDEAGRMDAE"
     gene            2503..2724
                     /locus_tag="VGB76_17765"
     CDS             2503..2724
                     /locus_tag="VGB76_17765"
                     /inference="COORDINATES: protein motif:HMM:NF041212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HEY0100798.1"
                     /translation="MGIVIYVKPGCPYCQQARDHYNSQGIAFTEYDAQNDRQRRKEMF
                     AYSDGDPTVPCIVEDGVYVGSGWGTPPRG"
     gene            2915..>3231
                     /locus_tag="VGB76_17770"
     CDS             2915..>3231
                     /locus_tag="VGB76_17770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0100799.1"
                     /translation="MLGSTLLNYRITEKLGSGGQGTVYKAVDTRLGRTLVVKVLSPEL
                     TSRESNLKRFEREAQLASSLDHPNICIIYGLHEEGGLHFITMQYIEGKNVRQLVKGRP
                     LELR"
BASE COUNT          647 a         1001 c          954 g          629 t
ORIGIN      
        1 tgaagaccgt gatgcaatgc tccgagcgca ggcgttcgac aaactccgcg cgcgcagaga
       61 ggcgtctgcc gcgatggacc agcgtgacgg tcgggcaaac ttccgccagc agcagcgcat
      121 tctccgccgc cgcgtcgccg ccgccgacga cgcacacgtc tcgcccggcc agcaactcgc
      181 ggtcgcgcgt cgcggattcg acgacgccgc gccccgcgag ttccgcctcg cccggaatcc
      241 ccagacgcct gcgccgcacg cccgtcgcga tcatgagact gatcgcctgc aacgcttcgc
      301 cgctgcgtag ctgaacgcgc cgtgcgcgca ggtcaacgct ctcaatctcg gtttcagtcc
      361 acaggtcaaa ctcgcaatct tcaacctgcg cggcgaagag atcgcgcaac tcgcgcccgt
      421 tcccggcgcg cacgcccatg taattattga ccgggttata gacgctcagc agttggccgc
      481 cgatctccga cccggcttcg agcacgagcg cgtccagacc taactcttcg caccataacg
      541 ccgccgacaa gcccgccgcc cccgcgccga tgatgatcac gtcgtacata aaaaagctgc
      601 cgttagttcg tcgccgctcg ccggttgtcc gcagtccttt gcctgtctct aactcgaatc
      661 tttagaggct ggcgcatgag ttacgaaaca actgatagcc ggtcaacggc aactgaccgc
      721 tgttcggcct gctcgcgcag ggcggcgcgc agttcgtctg cgacctgttc gggcgcgagg
      781 cttgaggcgc gaagcaggtt gtctgccccg gcgaagttga aagccgaact caagccctcc
      841 gtatcaacct gccagcgtcc ctctgcgttc tgttccaact cgccgagacg cgcgagcagc
      901 ggcgcgaaat cataacggct gaccgccggg cgcaccgtcg tctccgccca tgaataatag
      961 ggatagtcca cgcgatagcc ccacgcgcgt tcgagcggga tggcgatgag atagatgcgc
     1021 ccgcgcgcgt gctcggtgat ggccgtgtag ggcgcggcgt ggcgcgtcgg cttgaagccc
     1081 tgcgcgccgt agagttcgag gctcaaggtg acgacggaca gacccgtctc cgggtcttcc
     1141 tccacgcgcg acgcaccgcg cgcaaaactc tccagagagg cttcgatctt cgcccactcg
     1201 tcgtgccaga gatgttcgta ataattagtg cgcgtgatga cgcgttccac ttcgggcagc
     1261 acgagcgcgt aggcgcgctc ttcatccacg gacgcgtctc cggcgaggtg gcgcgcgagc
     1321 ggcgaggcga cgcccgcatc cggcgcaaac ccgtcgccgc cctgcaagac gaggctggcg
     1381 cggatcgcct gcgcgcccgt gttcgcggag aagtcgcccg cttcggcggc ggcgatgagc
     1441 gcgtcgcgca ggtcgagcgc acgttcgccc gtgagcatcg tccagacgga gagcacgccg
     1501 tcggtatcga aatgattgtt ggtgacgagt tcgatcccgc gcgtcaactc gtggcggtcg
     1561 ggcgaggcga cgacgttcaa ggcgatctcg gtggaactgt cggctttaac ttgcgcgggc
     1621 gtgcggtttc cctgccagtg tgagaagtgg acgctctcgg ggacgagtcc gtcaaccgaa
     1681 agtttcggca cgccgtccag tcccgcgtga taatactcga agcgcatacg tgcgaatcct
     1741 ttcatgcggc gcgtgtggaa atggtctttc agaacttatc aaacggaagt gtagcgcagg
     1801 cggagaaaag gatgaagcag gaacgatgaa cgatgaacga tgaataagaa aaccgcttga
     1861 tagagtgaat gcttttattc cgcatccatc cttcctgctt catccttcgc tttgttgatg
     1921 cctacgagtt tgacgtgctc gttgaggcgg gtgatgctca agccctcgcg cactttgaag
     1981 cggcaggaga gggcggtctt atcgtcgcgt gcgggttccc cttcgtctcg ataccagaac
     2041 tggcaattgg tgcagtcgcc gttccagcag aaatctccgt aggagatgct ccgcatcgag
     2101 agaaactgga agcagcgcag caggcggttg ttctccggca cttccacgcg acgaccgaga
     2161 atctcaatcg taatcaatcg ctcgtatggt tcaaacagat cgctgtcaat caagcgtgtt
     2221 cgactctgcc tttcccggcg gcctccggca aaactttgcg cgggagtata cctgtaaagc
     2281 agtgacaagt gccaagagca gtgacgagtg acgagtgacg agccggacga acggggtatt
     2341 gtgaatagtc ggcttagaca gacacctgtg ctccgtttgc gactaaagat tcacttgttt
     2401 ttacctcgtc acctgtcacc tgtcactcgt cactgctctt agcactcgtc actcttcact
     2461 gttcttgtta aactaagtgt gcgaagggag gtcgaacata aaatgggaat cgtgatatat
     2521 gtgaagccgg gctgccctta ttgccagcag gcgcgcgatc attacaattc gcaggggatc
     2581 gcgtttacgg aatacgacgc gcagaacgac cgtcaacggc gcaaggagat gtttgcttac
     2641 tccgacggcg acccgaccgt gccgtgcatc gtcgaagacg gcgtctacgt cggttcgggc
     2701 tggggcacgc cgccgcgcgg ttgaacggtg cacgactaag gctggccgtg cgccagtgaa
     2761 tgaagccggg gcaggggtca agggggagag gggacaggtg aagagattca gccgagccag
     2821 aggttttctt ccctttgccc cttgccccct accctttccc ctatttttat ctttgccatc
     2881 ccccccacct tcggagttag aatagtaatc atcaatgcta ggttcaactc tgctcaacta
     2941 ccggatcaca gaaaaactcg gctcaggcgg gcaaggcaca gtttataaag ccgtcgatac
     3001 caggctcggg cgcacgctcg tcgtcaaagt gctctccccc gaattgactt cgcgcgagtc
     3061 gaatctcaaa cgcttcgagc gcgaagccca actcgcatct tcgctcgatc acccgaacat
     3121 ctgcatcatc tacggcctgc acgaagaggg cggcctgcac ttcatcacga tgcagtacat
     3181 cgaaggcaag aacgtgcgcc aactcgtcaa aggccgcccg ctcgaacttc g
//
LOCUS       DASWNS010000516        12675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_201905, whole genome shotgun sequence.
ACCESSION   DASWNS010000516 DASWNS010000000
VERSION     DASWNS010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201905"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..716)
                     /gene="lexA"
                     /locus_tag="VGB76_17775"
     CDS             complement(90..716)
                     /gene="lexA"
                     /locus_tag="VGB76_17775"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY0100800.1"
                     /translation="MQPRTQRQKEILDYIQRFIERHGYEPSYAQIARHFGIASKATIA
                     KHIEALEKRGLLARSHANGSFGLNVHVEEVAGDALCEVRLLGRVAAGAPLETVDDNGE
                     TIGVPRFLLGRVRPERVYALRVSGDSMIDEHICDGDIALIENRVDARDGEIVVALVEG
                     TRATLKRLYRFGREVELRPANSQLAPIRLPAAQVQVQGIFRGLLRPAA"
     gene            complement(867..2306)
                     /locus_tag="VGB76_17780"
     CDS             complement(867..2306)
                     /locus_tag="VGB76_17780"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEY0100801.1"
                     /translation="MATTAAIGHEDRHPKGLYVLFATEMWERFSFYSMLAMFTLYLRD
                     PNEGFGWSAAEATSLYANYLMFVYLSPLVGGFIADRFTGYRRAVMIGGLFFIAGHLLL
                     SFSSLAAVYAALTCLVIGNGFFKPNVSTMVGNLYPEGSHLKDRAYNIFYMGINIGAFL
                     APLIAGWVRAKWGFHPAFAVAAGGMVISVWILFQFKKHIHEPGRNLTAPATATPTAAT
                     RSGDTAADTAATATDAPPHGVSHQDEAATRQGVMDAVPEWKRIVALCVIFAVVIVFWM
                     VFHQNASTLTYFADANTDWTTIFSPETAAESGGIISNSINPFWVITLTFPLVWFWGFL
                     DKRGLEPSTPTKMAFGMTLTALSFFILYFAAKSGEATALGGDPYAFRVSPMWLVLSYF
                     VVTLGELMLSPMGLSLVSKVAPIRLRGLMMGGWFVATAMGNKLTQIGVFWDRWLHSTF
                     FLVLGAMALVMAVVLFLLLKPLKKAMPGV"
     gene            complement(2441..3193)
                     /locus_tag="VGB76_17785"
     CDS             complement(2441..3193)
                     /locus_tag="VGB76_17785"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEY0100802.1"
                     /translation="MSLRDVAACALSVLLLFGLSLAHASAQEQEPQQRQRRVNAAPTK
                     KEEEAERKAESAAKVFDQIMNARDNAIPKELVDKAEAVAVFPGVLKAGFVVGGRGGSG
                     LISRRLKGGWSAPAFFTMRGGSFGAQIGAAKTDFVLLFMNESAVQGLLEDKFEMGGEA
                     SAAAGPVGRTASATTNLTLDAGILSYSRSKGLFAGLELKGAVVNPDNKLNEAVYGMKA
                     DRLLSPTDQLRVERAPEGVRIFPLTLARYSRR"
     gene            complement(3249..3974)
                     /locus_tag="VGB76_17790"
     CDS             complement(3249..3974)
                     /locus_tag="VGB76_17790"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEY0100803.1"
                     /translation="MKSFRRKLICAILFGALALGGTTFEAAAQSNKKEIEAREQANKA
                     AKVFRQIMSTPERAIPRELIDKAEAVAVFPDVIKAGFLLVGGRGGRGVISRRIPGGWS
                     APAFFNLGGGSIGPQLGASSTDFVLLFMNKDAVNGLLEDKFEIGGEGSIAAGPVGRAA
                     SASTDLQLRAGILSYSRSKGLFAGLELKGVVINPDNKDNEAVYGMKAKELLTGEGLKR
                     GGKPWIPPSVRAFPLQLSRYSSK"
     gene            complement(4221..4748)
                     /locus_tag="VGB76_17795"
     CDS             complement(4221..4748)
                     /locus_tag="VGB76_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100804.1"
                     /translation="MSGAGNDNKDRWVYGMRVLSKQIPRAAVRTTLTYKFHPRLTAGV
                     EYNPGADEVAPIANLLVLTETRRRPALMLGTSSDRIGTPSGQSFYMTLSKSLRRETGL
                     PIAPYVGMAYGTFQDRLLPVGGISIQLNKKFSALITHNGINLHPILNYAYNRHAFSLI
                     MVKSRYPGVSYSVSF"
     gene            complement(4776..5729)
                     /locus_tag="VGB76_17800"
     CDS             complement(4776..5729)
                     /locus_tag="VGB76_17800"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0100805.1"
                     /translation="MKRLSMSVVLLSLAFAAGAVRAQEVAAAGNAPVKLEGQIVCSVC
                     WFEAEDRSKTPYGTAADIKCAEDCAAKNVDAALAVREGETNKFALYLLEDGRFDKRAK
                     DWLAFMGKRVEVAGTTRASKDKQYLKVDALKVVEGGGESAQASVLQNAKVLGTQVELA
                     LPDLFGAEQKLSAYRGRVVVLNFWATYCEPCRREMPDLAEVQNSYAALGVQVIGASAD
                     VAEAKPAVLKFIKETKLNFPVWLGASVADMERFGLAPALPGTVIVGRDGKVVWHKSGV
                     IKAAEVKQEIDKLLAQAAKEEKSRKRELAAAHEEHASSVPS"
     gene            complement(5785..6405)
                     /locus_tag="VGB76_17805"
     CDS             complement(5785..6405)
                     /locus_tag="VGB76_17805"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HEY0100806.1"
                     /translation="MKPIHLTTKKRLHLTLACVLLFAASILLQACGGASSEQTASQSQ
                     RTTSASRSTAATTTTPQPQTPSAVAPQAAHDTGGSTAAATQADGHEGHSHGAPGRVPR
                     YAKSGADLKNLPPVLPASQFTGMTRMAYAAVKEIPETIAQLPCYCHCDEGFGHKSLQT
                     CFVDDHAAHCAVCVEEALMAYKLQKEERLNPAQIRERIIAKYGSAN"
     gene            complement(6445..8442)
                     /locus_tag="VGB76_17810"
     CDS             complement(6445..8442)
                     /locus_tag="VGB76_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100807.1"
                     /translation="MHTSKFSHDDVSRLLLLRVFAVAVLLVAASAGVRAAQQHDQHGG
                     HEGHTASKKSAPKKSTSKKAAPRRRVAARRKPTPRRRAQATPARQPSVKDGATHEGHD
                     MHGTHGAAQPAATPAPPAPETPIPASNLTNETVPVATPSPAHTHLHTTQPSAPTPAAQ
                     QSAPNAPAQPAKPSEVDHSTHAPAPTGTGAPDKTVAPAAVDHSMHRGHDAAASKEGDA
                     EKVKTVSEAEVSELMVMSGETMGIRNGGSRDNFTPMGQIGSGTAWQPASTPMYMLHKS
                     SGDWLFMFHYEAKIGVNAQGGERGITKFESQNWFMPMAYRRVGAGTLQLRAMVSIEPL
                     TFSGAGSPQLFQTGEVYQGRPIVDAQHPHDLFMELSAQYTVPIGERATWFTYFGFPGE
                     PALGPVAFMHRASASENPTAPLTHHLQDSTHIAFGVLTTGFSYRWFKFEGSLFNGREP
                     DERRYNFEAHPFNSRSARLTFAPNKNWAFQVSHGFLRNPEQLHEGDVRRTTASVQYNR
                     AFARGNLAAAFIWGRNHESHDGELFNLNGYTFETTANFLDKNYLYTRLELVDKSALLS
                     HEEEDRLGFAHDSHPQFRIGAYTFGYVRDVWNTDKLSVGVGGDLTFYSKPAILDSIYG
                     NNPKSYKFFIRLRPARMKMSDHSGHGATHNSDEHGAAHDKH"
     gene            complement(8599..9168)
                     /locus_tag="VGB76_17815"
     CDS             complement(8599..9168)
                     /locus_tag="VGB76_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100808.1"
                     /translation="MRRTALIFGLLLTLTAGVWSSALAAVVYACCADETSAASTVAAL
                     PAPDEHDCCRARFAEPGAPPAEATGHAHEAATHEDELSPAPQVESSHAGMDCANSKEP
                     ESDARVTAFGERLCSECCVGRGNQVPTTATFSAPEQNRAKRDAASVYVNARELFAPGT
                     QGVAHLSPSQHAPPASRERRHILISVFLI"
     gene            complement(9300..9680)
                     /locus_tag="VGB76_17820"
     CDS             complement(9300..9680)
                     /locus_tag="VGB76_17820"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY0100809.1"
                     /translation="MDTQEIFVLAAGIGLIALVLWYFFAGEGVRAAASVTATGVQRLK
                     VLVKGGYTPDVIVVKRGAPVELDFYRDETNSCTEQVIFGDLQISRMLPAFKTTRIEFT
                     PARAGTFTFNCGMNMVRGKLIVED"
     gene            complement(9738..12122)
                     /locus_tag="VGB76_17825"
     CDS             complement(9738..12122)
                     /locus_tag="VGB76_17825"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY0100810.1"
                     /translation="MNAITSTEETTKKSATNNVAATPDAHAEVRHLPEAQGGSAERVD
                     LSLAGMTCNACARRIERKLSNAQGVRSANVNFATARATVEYDPHATGAAQLIETVREI
                     GYGASDAGEEGAAEREQDAERAEQQTLRRRFLVAAVFSAPVLLIAMSHGSITLFNVSW
                     INWAQLALTLPVIFYSGWPFYQGAWTSLKHRAADMNTLIATGTGAAFIYSLVVTIAPH
                     LIARTHDASMGGMHEGGTMPAPVYYEAASVIIALLLLGRLLEARARGKTSEAIKRLLG
                     LQPRTARVVREDGSEVDLPVEAVGVGDTIVVRPGERIPVDGIVIEGASAVNEAMLTGE
                     SMPVEKSAGAEVIGATINGTGSFRFRATKVGRDTVLQQIVRMVEQAQGGRAPIARLAD
                     IISGYFTPIVICIAILAFVVWFIAAPVESRLSYALVSFVSVLIIACPCALGLATPTAV
                     MVGTGRGAEHGILVKGGEALETAHRLTTIVLDKTGTITRGQPALTDVLTVGDFDEDEL
                     LRLAAAAERGSEHPLGEAIVRRAAERGLAQANVARFNALAGHGVEAEVEGRRVLIGNL
                     KLMSERGVAALDDLRERAAILTGAGKTAVYVAVEGAAAGVLAVADETKAESKEAVAAL
                     ERLGLEVVMITGDNPQTAEAVAREVGIKRVLAEVLPEGKAAEVQKLQAEGKRVAMVGD
                     GINDAPALAQADIGIAIGTGTDVAIEASDITLIRGDLRGVVTAIALSRQTIKVIKQNL
                     FWAFVYNTLGIPLAAGAFYPFMGWLLSPVVASAAMALSSVSVVANSLRLKRFRL"
     gene            complement(12119..>12675)
                     /locus_tag="VGB76_17830"
     CDS             complement(12119..>12675)
                     /locus_tag="VGB76_17830"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HEY0100811.1"
                     /translation="LDSVVGYRVYNVSMLKTARQFGGQVEGRQTLRIGEVAAATGLSV
                     EALRFYERRGLLGRPARTASGYRAYDASVVERLAFIKRAQAIGFSLDEIAEILEMRGR
                     GEAPCQEVREAARRKLSELDERLRELRRYRNELARTLDDWDERGIEEGQFCGLIEHSH
                     VHAPQAAKATNAPAVKRTNKAERR"
BASE COUNT         2219 a         4083 c         3913 g         2460 t
ORIGIN      
        1 ggggcgcaga gagttgattg aactctctgc gccccttctg catgatcgct acgcgtctac
       61 ggcggtgact gtctgctgcg gtgactattt caggccgccg gtcgcaacaa gccgcgaaag
      121 atgccctgca cctgaacctg cgcggcgggg aggcggatcg gcgcgagttg tgagttggcc
      181 ggacggagtt cgacttcgcg cccgaagcga tagagccgtt tgagcgtggc gcgcgtgcct
      241 tcgacgagcg ccacgacgat ctcgccgtcg cgtgcgtcca cgcgattttc gatgagcgcg
      301 atgtcgccgt cgcagatgtg ctcgtcaatc atcgagtcgc cggagacgcg caaagcatag
      361 acgcgctccg gtcgcacccg acccagcaga aaacgcggga cgccaatcgt ctcgccattg
      421 tcgtcaaccg tctccagcgg cgcacccgcc gcgacgcgcc cgagcaggcg cacttcacac
      481 agcgcgtcgc ccgccacctc ttccacgtgg acgttcaagc cgaaagaacc gttggcatgg
      541 ctgcgcgcga gcaggccgcg cttctccaac gcttcgatat gcttggcgat ggtcgccttc
      601 gaagcgatgc cgaagtggcg cgcgatctgc gcgtacgaag gttcatagcc atgccgctcg
      661 atgaagcgtt ggatataatc caagatttcc ttctggcgtt gggtgcgggg ttgcataagt
      721 cttgccgatc ctttcagacg cgagcgggcg cgattcccgc gtctcctaaa agccttgcga
      781 cgctgttgcg gcgagggcgg gcgcgctcct cagacggatt ctaaagtcgc cttcgccgca
      841 gcgtcaaggt ttaccactcg tgccgtctaa acacccggca tggccttctt cagcggcttg
      901 agtaacagga acaacacgac ggccatgacg agcgccatcg cgccgagcac gaggaagaag
      961 gtagagtgca gccagcgatc ccagaagacg ccgatctgcg tcagcttgtt gcccatcgcc
     1021 gtcgccacga accacccgcc catcatcaac ccgcgcaggc gtatcggcgc gaccttcgag
     1081 acgagcgaca gccccatcgg tgacaacatc aactcgccga gcgtgacgac gaagtaagag
     1141 aggacgagcc acatcggcga cacgcggaac gcataagggt cgccgccgag cgcggtcgcc
     1201 tcgccggact tcgcggcgaa gtagagaatg aagaaggaga gcgcggttaa agtcatgccg
     1261 aaggccatct tggtcggcgt cgaaggctca agcccgcgct tgtcgaggaa gccccagaac
     1321 cagacgagcg ggaacgtcag cgtgatcacc cagaacggat tgatcgagtt tgagatgatg
     1381 ccgcccgatt ctgccgccgt ctccggcgag aagatggtcg tccagtcggt gttggcgtcg
     1441 gcaaagtagg tcagcgtcga ggcgttctgg tggaagacca tccagaagac gatgacgacg
     1501 gcgaagatga cgcagagcgc gacgatgcgc ttccattcgg ggacggcgtc catcacgccc
     1561 tgtcgcgtgg cggcctcgtc ctgatggctg acgccgtgtg gcggcgcgtc ggtggcggtg
     1621 gccgcagtgt cggcggcggt gtcgcccgaa cgggtcgcgg cggtcggcgt ggcggtcgca
     1681 ggcgcagtca ggttgcgtcc cggctcatgg atatgcttct tgaactggaa gagaatccag
     1741 accgagatga ccatgccgcc cgccgccact gcgaaggccg ggtggaaacc ccacttggcg
     1801 cgcacccaac cggcaatgag cggagcgagg aacgcaccga tgttgatgcc catgtaaaag
     1861 atgttgtagg cgcggtcttt gaggtggctg ccctccgggt agaggttgcc caccatcgtc
     1921 gagacgttcg gcttgaagaa gccgttgccg atcacgaggc aggtgagcgc ggcatagacg
     1981 gcggccagcg aactaaagga gagcagcagg tgtccggcga tgaagaagag gccgccgatc
     2041 atcaccgcgc ggcgatagcc cgtgaagcgg tcggcgatga agccgccgac gagcggcgag
     2101 agatagacga acatcaggta gttagcgtac aggctggtcg cctcagccgc cgaccagccg
     2161 aagccctcat tcgggtcgcg caggtagagc gtgaacatcg cgagcatcga gtagaagctg
     2221 aaacgctccc acatctcggt ggcgaagagc acatacaaac ccttcggatg tctgtcttcg
     2281 tgtcctattg ctgctgttgt agccattttg tcccaagctc ctcttcaaaa tttgtgatcc
     2341 caaatcttca acatatctgg ccgccgtctc ttgaccaaac cgcgttaaca gcagcgccct
     2401 ccagcgcaag aatccttccc gcaacgcgtg cggcgcgccg tcagcgtctc gaataacgcg
     2461 caagcgtaag ggggaagatg cgcacgcctt cgggcgcgcg ttcgactctg agttgatccg
     2521 tcgggctgag cagtctgtct gctttcatgc cgtagacggc ctcgttcagt ttgttgtccg
     2581 ggttgacgac cgcgcctttg agttcgagtc cggcgaagag tcctttgctg cgggagtagg
     2641 aaagaatgcc tgcgtcgagc gtgaggttgg tggtcgcgct cgcggtgcgc cccacggggc
     2701 ctgccgccgc gctcgcctcg ccgcccatct cgaatttgtc ttcgagcaga ccctgcacgg
     2761 cggactcgtt cataaagagc agcacaaagt cggtcttggc cgcgccgatc tgcgcgccga
     2821 aactcccgcc ccgcatcgtg aagaaggcgg gcgcgctcca accgcctttc aagcggcgac
     2881 tgatgaggcc acttccgccc cgtccgccca cgacgaagcc cgccttgagc acgccgggaa
     2941 agaccgcgac cgcctcggcc ttgtcaacca actctttcgg aatcgcgtta tcacgcgcgt
     3001 tcataatctg gtcgaagacc ttcgccgcgc tctcggcctt gcgttcggcc tcctcttcct
     3061 tcttcgtcgg cgcagcgttc acacgccgct gccgctgctg cggctcctgc tcctgtgccg
     3121 acgcgtgcgc aaggctcagg ccaaacaaca acagcacgct cagcgcgcac gccgccacgt
     3181 ctcttaacga caactgcttc attccttcgg gcctttcaga tttcactttt aaactttagt
     3241 cgcaaaccct acttgcttga ataccgtgac agttgcagcg ggaaagcgcg cacgcttggc
     3301 ggaatccacg gtttgccgcc gcgctttaag ccttcgcccg ttaacagctc tttcgctttc
     3361 atcccgtaga cggcttcgtt gtccttgttg tccgggttga tgacgacgcc cttgagttca
     3421 agcccggcga agagtccttt gctgcgggag taggaaagaa tgcccgcgcg aagttggagg
     3481 tcggtggatg cgctcgccgc gcgcccgacg gggcctgccg cgatgctgcc ctcgccgccg
     3541 atctcaaact tgtcttccag taaaccgttc accgcgtctt tattcataaa gagcagcacg
     3601 aagtcggtgg aagacgcgcc gagttggggg ccgatgctgc cgccgccgag gttgaagaag
     3661 gcgggcgcgc tccaaccgcc cggaatgcgc cggctgatga cgccgcgacc gccgcgtccg
     3721 ccgacgagca ggaagcccgc cttgatcaca tcgggaaaga ccgcgacggc ctccgccttg
     3781 tcgatcaact ctctgggaat cgcacgctcc ggcgtggaca tgatctggcg gaagactttc
     3841 gcggccttgt tggcctgctc gcgcgcctct atctcttttt tattgctctg cgccgccgct
     3901 tcgaaggtcg tcccgccgag ggcgagcgca ccgaagagga tcgcgcagat cagttttctc
     3961 cgaaatgatt tcatgcttct tctccttagc aaactaaaac agttcggcga cgacaccggg
     4021 ggagaacaag cgtgttcgct ttccagcggg gagggatcaa cagattgtgt cgcgccttag
     4081 tttatgacgc gcacatctta caggcagaag ggggaaaggg aaaaggggaa aggtgaaggg
     4141 ggaggataaa aactgaagcc gagcactatt tctcacgcgc tcaacttcta tcactttccc
     4201 cttccccttt tcccgcgcgc ctaaaacgag acgctgtagc tcacgcccgg atacctgctc
     4261 ttgaccatga tgagcgagaa ggcgtggcgg ttgtacgcat agttcaggat cgggtgcagg
     4321 ttgatgccgt tgtgcgtgat gagggcggag aattttttgt tgagttgaat gcttatcccg
     4381 ccgacgggca gcagcctgtc ttgaaacgtg ccgtaagcca tgccgacgta aggcgcaatc
     4441 ggcaaccccg tctcgcgccg caggctcttg ctcagggtca tgtaaaagga ttgaccggaa
     4501 ggcgtgccga tgcgatccga actcgtgccg agcatcagag cgggacgccg cctcgtctcc
     4561 gtcaagacga gcaggttggc gatgggcgca acttcatccg cgcccggatt gtattccacc
     4621 ccggcggtca gtcgcgggtg aaacttgtag gttaaagtcg tgcgcacggc ggcgcgcgga
     4681 atctgcttcg agagcacgcg catcccgtac acccagcggt ctttgttgtc gtttcctgcc
     4741 ccgctcacgg cgcggccttc tcccggtcaa gacggtcagg aaggaacgct cgaagcgtgc
     4801 tcctcgtgcg cggcggccaa ctcgcgcttg cgcgacttct cttccttcgc cgcctgcgcc
     4861 agcagtttgt cgatctcctg cttcacttcc gccgccttga taacgccgct cttgtgccag
     4921 acgaccttgc cgtcgcgccc gacgatgacc gtgccgggaa gcgcgggcgc gagtccgaaa
     4981 cgctccatgt cggccacgct tgcgccgagc cagacgggaa agttcaactt cgtctccttg
     5041 atgaacttca acaccgccgg tttggcctcc gccacgtcag ccgacgcgcc gatcacctgc
     5101 acgccgagcg cggcgtaact gttctgcact tccgcgaggt cgggcatctc cctgcgacac
     5161 ggctcgcaat aggtcgccca gaaattgagc accacgacgc gcccgcgata cgcgctcaac
     5221 ttctgctccg cgccgaagag gtctggcagc gcgagttcaa cctgcgtgcc gagcactttc
     5281 gcgttctgca agacggaagc ctgcgcgctc tcgccgcccc cctccacgac cttcaacgcg
     5341 tcaaccttca aatactgttt atctttcgag gcgcgcgtcg tgcccgcgac ttccacacgc
     5401 ttgcccatga aagcgagcca gtccttcgcg cgcttgtcga agcgtccgtc ttcgagcagg
     5461 tagagcgcga atttattcgt ctcgccctcg cgcacggcga gcgcggcgtc aacgttcttc
     5521 gcggcgcaat cttccgcgca tttaatatcc gccgccgtgc cgtaaggcgt ctttgaacgg
     5581 tcttccgcct cgaaccagca gaccgagcag acaatttgac cttcgagttt caccggcgcg
     5641 tttcccgctg cggctacttc ctgcgcccgc accgcgcccg ccgcaaaggc cagcgacaac
     5701 agcacgacgc tcatcgacaa tcttttcatc acaacatttt ccccctcaga cccgactgcc
     5761 cggcgcaatc agagatggtc aagttcaatt cgcgctgccg tatttcgcaa tgatgcgttc
     5821 gcgtatctgc gcgggattca ggcgttcctc tttttgcaac ttgtaggcca tcagagcctc
     5881 ttcgacgcag acggcgcagt gtgccgcgtg gtcgtccacg aaacaggttt gcaggctctt
     5941 gtggccgaag ccttcgtcgc aatggcagta gcacggaagc tgcgcgatgg tttcgggaat
     6001 ctctttaacc gccgcgtagg ccatccgcgt cattcctgtg aactgcgacg cggggagcac
     6061 gggcggcaga ttcttcaagt ccgcgccgct cttcgcgtaa cgtggcacgc gtcccggcgc
     6121 gccgtgcgaa tgtccctcgt gcccgtccgc ctgcgtcgcc gccgcagtgc tcccgcccgt
     6181 gtcatgcgcg gcctgcgggg cgacggcgga gggagtttgt ggttgcggcg tcgtcgtcgt
     6241 cgccgctgtg cttcgggacg cggaggtcgt gcgctgcgac tgcgaggcgg tctgttcgga
     6301 cgacgcgccg ccacacgcct gcaataagat tgacgccgcg aataacagga cgcacgcgag
     6361 cgtgaggtgg agtctcttct ttgtcgtcag gtgaattggc ttcatgtgaa attgtctctc
     6421 cttaaagtgt gtctggaatt gagatcaatg tttgtcgtgc gccgcgccgt gttcgtcgct
     6481 gttgtgtgtc gcgccgtggc cgctgtggtc gctcatcttc atgcgcgcgg ggcgcaggcg
     6541 tatgaagaac ttgtacgact tcgggttgtt gccgtagatc gaatcgagga tcgctggctt
     6601 ggaatagaaa gttaaatcgc cgccgactcc gacagacagc ttatccgtgt tccacacgtc
     6661 gcgcacgtag ccgaaggtgt acgcgccgat gcgaaactgc gggtgcgagt cgtgcgcgaa
     6721 gcccagacgg tcttcttcct catgactaag aagtgcgctt ttgtccacga gttcgagccg
     6781 cgtgtagaga taattcttgt cgagaaagtt cgcggtcgtc tcgaaggtgt agccgttcag
     6841 gttgaagagt tcgccgtcgt ggctttcgtg attgcgcccc cagatgaagg ccgccgccag
     6901 gttgccgcgc gcaaacgcac ggttgtattg cacggaagcg gtcgtgcgcc gcacgtcgcc
     6961 ctcgtgcagt tgttcggggt tgcgcaggaa accgtgactc acctgaaaag cccagttctt
     7021 gttcggcgcg aaagtgagac gcgccgagcg ggagttgaac gggtgcgcct cgaagttgta
     7081 gcgacgctca tcgggttcgc gcccgttgaa gagcgagcct tcgaatttga accagcgata
     7141 gctgaagcct gtggtgagca ccccgaaggc gatgtgcgtg gagtcctgca agtggtgcgt
     7201 gagcggcgcg gtcgggttct cggaagctga cgcgcggtgc atgaaagcga cgggaccgag
     7261 cgcaggctcg ccgggaaaac cgaaatacgt gaaccacgtc gcgcgctccc cgatgggcac
     7321 cgtgtactgc gcggagagtt ccatgaaaag gtcgtgcggg tgctgcgcat cgacgatggg
     7381 gcggccttga taaacttcgc cggtctgaaa aagctgcggc gagcctgcgc cggaaaaggt
     7441 caacggttcg atactgacca tcgcacgcag ttgcagcgtc cccgccccga cgcgcctgta
     7501 ggccatcggc ataaaccagt tctgcgattc aaacttcgtg atgccgcgct cgccgccctg
     7561 cgcgttgacg ccgatctttg cctcgtagtg aaacatgaag agccaatcgc cggacgactt
     7621 gtgcagcatg tacatcggcg tggaggcggg ctgccatgcc gtgcccgatc cgatctgccc
     7681 catcggcgtg aagttgtcgc ggctcccgcc gttgcgaatg cccatcgtct cgccggacat
     7741 caccatcaac tcgctgactt ccgcttccga gacggttttg accttctccg cgtctccctc
     7801 cttcgacgcc gccgcgtcgt gccccctgtg catgctgtga tcgactgcgg cgggcgcaac
     7861 cgtcttatcc ggcgcgcccg tccctgtggg cgcgggtgcg tgcgtgctgt gatcgacttc
     7921 cgagggtttc gcgggctgtg cgggcgcgtt tggcgcagac tgctgcgcgg caggcgtcgg
     7981 ggcggacggc tgcgtcgtgt gtagatgcgt gtgcgcgggc gaaggcgtcg caaccggcac
     8041 agtctcgttc gtcaggttcg acgccgggat gggcgtttcg ggtgcgggcg gcgcgggcgt
     8101 cgccgccggt tgcgctgcgc cgtgagtgcc gtgcatgtca tggccttcgt gcgtcgcgcc
     8161 atctttgaca gagggctgcc gcgcgggcgt ggcttgcgct ctcctgcgcg gcgtgggttt
     8221 gcgtctggct gccactctgc gtctcggcgc agccttcttc gacgtagatt tcttcggcgc
     8281 agacttcttc gacgcggtgt gtccttcgtg cccgccatgt tgatcgtgct gctgtgctgc
     8341 ccgcacgcct gcgctcgcag cgacgagcag gacggcgacg gcgaacacgc gcagcagcag
     8401 aagcctgctc acgtcgtcgt gcgaaaattt cgatgtgtgc atgattacct ctcgaacccc
     8461 ttcggaatcg tgacgggaag caaaatcttc cgcgtcgcgc gtccgttcaa tatttttgct
     8521 ttgacgcttg aatagggcgt gtgcctccgg ccatccattc gcgcgggcgc gcgaagccgg
     8581 agacgtttcg agagagggtt agatgagaaa gacgctgatc aagatatgtc tacgctcacg
     8641 cgacgcaggc ggtgcgtgct gggagggcga aagatgcgcg acgccttgtg tgccgggcgc
     8701 gaacagttca cgcgcgttca cgtagacgct ggcggcgtcg cgcttcgccc tgttctgttc
     8761 gggcgcgctg aacgtggcgg tggtcggcac ctgattgccg cgacccacgc agcattcaga
     8821 gcacaaacgc tcgccgaaag ccgtaacccg cgcgtcgctc tccggctctt tcgagttcgc
     8881 gcaatccatc cctgcgtgcg aactttcaac ttgaggtgcg ggcgaaagct cgtcttcgtg
     8941 cgtcgctgcc tcgtgcgcgt gtccggttgc ttccgcaggc ggcgcgcccg gctctgcgaa
     9001 gcgggcgcgg caacagtcgt gctcgtcggg cgcaggaagc gcggcgacgg tgctggccgc
     9061 gctcgtttcg tctgcgcagc aagcgtagac gacggcggcg agcgcactgc tccacacgcc
     9121 cgccgtgagc gtgagaagca agccgaagat gagagccgtt cgtcgcataa ctgttggaac
     9181 ttttcgctgc cgcgcctgag tttgacggtg gccgtcaatt taacatggat actctccggc
     9241 gcgctaattt gcagcgactc gaagagtaga tcgtcgccgt cgaagcttaa agatgcgcgt
     9301 cagtcctcga cgatcaattt gccgcgcacc atgttcatgc cgcagttgaa ggtaaacgtg
     9361 cccgcacgcg cgggcgtaaa ttcgatgcgg gtcgttttga aggcgggcaa catgcggctg
     9421 atctgcaagt cgccgaagat cacctgctcg gtgcaggagt tcgtctcgtc gcggtagaaa
     9481 tcgagttcga cgggcgcgcc gcgcttcacc acgatcacgt ccggcgtata gccgcccttg
     9541 accagcacct tcaaacgctg cacgcccgtc gccgtcacac tcgccgccgc gcgcacgcct
     9601 tcgcccgcga agaaatacca cagcaccagt gcgatcaaac cgatgcccgc cgccagcaca
     9661 aaaatttcct gtgtgtccat aatcttctcc tcaactcccg aaagttaagc gcaaatgacc
     9721 aaaccgcata ccaggcatca caggcgaaag cgtttcagcc gcaggctgtt ggcgacgacc
     9781 gagacggacg acagagccat cgccgcgctc gccacgacgg gcgagagcag ccaacccatg
     9841 aacgggtaga acgcccccgc cgcgagcgga atgccgagcg tgttgtagac gaacgcccag
     9901 aagaggttct gcttgatcac tttgatcgtc tgccgcgaga gcgcgatggc cgtgacgacg
     9961 ccgcgcaggt cgccgcgaat cagcgtgatg tccgacgctt caatcgctac gtcagtgccc
    10021 gtcccgatgg cgatgccgat gtctgcctgc gccagcgcgg gcgcatcgtt gatgccgtcg
    10081 cccaccatcg ccacgcgctt cccctcggcc tgcaacttct gcacctccgc cgccttcccc
    10141 tccggcaaca cttccgccag cacccgcttg atgcccacct cgcgcgccac cgcttccgcc
    10201 gtctgcgggt tgtcgcccgt gatcatcacg acttccagcc ccagacgttc gagcgcggcg
    10261 acggcttctt tcgactccgc tttcgtctcg tcggcgacgg ccagaacgcc agccgccgcg
    10321 ccctcaaccg cgacgtaaac ggcggtcttc cccgccccgg tcaaaatcgc cgcgcgttcc
    10381 cgcagatcgt ccaatgccgc gacgccgcgt tcggacatca acttgagatt cccgatcagc
    10441 acgcgccgcc cttcaacttc cgcctcgacg ccgtgacccg ccagcgcatt gaagcgcgcg
    10501 acgttcgctt gtgcgagtcc acgctcggcg gcgcgtcgca caatcgcttc gcccagaggg
    10561 tgctcgcttc cgcgttcggc ggcggcggcg agtcgcagca gttcgtcttc gtcaaagtct
    10621 ccgacggtca acacgtctgt cagggcaggt tgcccgcgcg tgatcgtgcc ggtcttatcg
    10681 agcacgatgg tcgtcaggcg gtgcgccgtc tccagagcct cgccgccttt gacgaggatg
    10741 ccgtgctccg cgccgcgccc cgtcccgacc atcacggcgg tcggcgtcgc caagcccaaa
    10801 gcgcacgggc acgcgatgat caagaccgac acgaaactga cgagcgcgta actcaggcgc
    10861 gactcgaccg gcgcagcgat gaaccagacg acgaacgcga ggatcgcgat gcagatcacg
    10921 atgggcgtga agtagccgct gataatatcg gcgagccgcg cgataggcgc acgccctccc
    10981 tgcgcctgtt ccaccatccg cacgatctgt tgcagcaccg tatcgcgccc gaccttcgtg
    11041 gcgcggaaac gaaaactccc cgtgccgttg atcgtcgcgc cgatcacttc cgcgcccgca
    11101 ctcttttcga cgggcatgct ctcgcccgtc aacatcgcct cgttgaccgc cgacgcgcct
    11161 tctatcacga tgccgtcaac cggaatccgc tcgcccggcc tgacgacgat ggtgtcgccc
    11221 acgccgaccg cttcgacggg caggtcaact tcgctgccgt cctcgcgtac gacgcgcgcc
    11281 gtgcgcggtt ggagtccgag caggcgtttg atggcttcgg aagtcttgcc gcgcgcacgc
    11341 gcttcgagca gacgaccgag cagaaggagc gcgatgatca cgctcgccgc ttcatagtag
    11401 acgggcgcag gcatcgtgcc gccttcgtgc atgcccccca tcgacgcatc gtgcgtccgt
    11461 gcgatgaggt gcggcgcaat cgtcacgacg agcgaataga tgaacgccgc gcccgtgccc
    11521 gttgcgatga gcgtgttcat gtcggcggcg cgatgtttga gcgaagtcca cgcgccctga
    11581 tagaaaggcc aaccgctgta gaagatgacc ggaagcgtga gagcgagttg cgcccagttg
    11641 atccatgaga cgttgaagag cgtgatactc ccgtgcgaca tcgcgatgag gagcacgggc
    11701 gcggagaaca ccgccgcgac gagaaaacgc cgccgcagcg tctgttgctc cgcgcgctcc
    11761 gcatcctgct ccctctcagc cgcgccttcc tctccggcgt cgctcgcgcc gtagccgatc
    11821 tctctgaccg tctcgatcaa ctgcgccgct ccggtcgcgt gcgggtcgta ttcgacggtc
    11881 gcacgcgccg tcgcaaagtt gacgtttgcg ctccgcacgc cctgcgcgtt cgagagtttg
    11941 cgctcgatgc gccgcgcaca ggcgttgcac gtcatcccgg cgagcgatag atcgacgcgc
    12001 tccgcgctcc cgccctgcgc ttcgggcagg tggcggactt ctgcgtgagc gtccggtgtg
    12061 gcggcgacgt tgtttgtcgc gctcttcttc gtcgtctctt ctgtgctggt gatagcgttc
    12121 atcgtcgttc agccttgttc gtccttttaa ctgcgggcgc attcgtcgcc ttcgccgcct
    12181 gcggggcgtg cacgtggctg tgttcgatga gtccgcaaaa ctgtccttct tcgatgccgc
    12241 gctcgtccca atcgtcgagc gtgcgcgcca gttcgttacg ataacggcgc agttcgcgca
    12301 ggcgttcgtc gagttccgag agtttgcgtc gcgccgcttc gcgcacctcc tggcagggcg
    12361 cttcgccccg cccgcgcatc tccagaatct ccgcgatctc gtcgagcgaa aagcctatgg
    12421 cctgcgcgcg tttgatgaag gcgaggcgtt caaccaccga agcgtcgtag gcgcgatagc
    12481 cggaagcggt gcgcgccggg cgaccgagca ggccgcgccg ctcgtaaaaa cgcagagctt
    12541 cgacgctcaa ccccgtcgcc gccgccacct cgccgattcg cagcgtttgc cgcccctcaa
    12601 cctgtccgcc gaattgtcgt gctgttttca acatggagac attgtaaacc ctgtagccca
    12661 ctacagagtc aagcc
//
LOCUS       DASWNS010000517         4706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_201981, whole genome shotgun sequence.
ACCESSION   DASWNS010000517 DASWNS010000000
VERSION     DASWNS010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201981"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..295)
                     /locus_tag="VGB76_17835"
     CDS             complement(35..295)
                     /locus_tag="VGB76_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100812.1"
                     /translation="MSTLPELHPSTSNRILNALTRAEHERLAPDLEPVILSAGEVIYH
                     PDQPITHVYCHRHASAKLCTFGARLSVATHFYRMIEVVPDAQ"
     gene            complement(749..1366)
                     /locus_tag="VGB76_17840"
     CDS             complement(749..1366)
                     /locus_tag="VGB76_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100813.1"
                     /translation="MEKRRVSGWLAAACCVLGLMLAQPAPVAFGQRRGSRAVEGGRQH
                     ITGTVVGVGGRFAGRTTSFDLIINRYTSDEEVRQLNAALQSGGQEELLRVLSRMDAGR
                     ITVGNGVGVDANAIISVPHENGTKLIVLYERNINIYEARAGARSTDYKFGYAEIFMRR
                     RNSEGTLIPAAKVRLRDGNTWEVEDFGVFPARLMGLQSRGGLGAR"
     gene            complement(1683..2210)
                     /locus_tag="VGB76_17845"
     CDS             complement(1683..2210)
                     /locus_tag="VGB76_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100814.1"
                     /translation="MYQLTARCKRLSLAFVLGLFAVAACPAQTPAAGNPANKTEKAAP
                     AALGKDKPATATATASTTPEEELAKTPAGKTVAKFFAAFNSGDLKQMQAFHESTSGNP
                     ENADKDFEVYERTGGLKLHSLSPSETKDKIALLVQTKKDGRWLAFEFTVGTEEPYPIT
                     GINARPAQPPAQASH"
     gene            complement(2463..>4706)
                     /locus_tag="VGB76_17850"
     CDS             complement(2463..>4706)
                     /locus_tag="VGB76_17850"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0100815.1"
                     /translation="SGERFTHALLANNGVVVAASLLERLRLSVGDEVKIGNSTFQIRG
                     AIGQEPGATGGFRIGPRVFIAREAIAQTGLTGFGSRARNRVLLKVPPAELNALVAELR
                     AGLKENLVSVRSYRDSQEGLNEQYTRSENYLSLTGLVILVLGGIGVSNVTRVFIEQKK
                     RTIAVLKCVGASSRVITGVYLAQVLALGLAGSLFGVVLARAALLFVSRRFAETLPPNM
                     SYELTWGAIAQGLMLGLLISLLFSALPLLRVRRIKPNMLLREDAAGGDNNGSDDEAQP
                     EAGWRGWWLRLRRFLSPRHALQSLRRIGAARSLVAFVVVGGLVLLAAWQAGSIRVGAF
                     FMAGLAATAFVLNLTAMLVIFLVRRARHIRSFPLRHAINSLYRPGNQTRVVVLAVGLG
                     AFLVIATQSLQRNLVREFDPAARAQLPNMFLIDVQTDQKNGVEELITQATGERPTLVP
                     TVRTRIAAINGQSIDVDDRGRRAQRGLLAREYVVTYRPQLEANETIVAGKFWDAAPSS
                     EPEISIEEGMRGLAGLELGGTITFDIAGRRLTARVTSIRRVDWRNSRTGFLILFRPGA
                     LENAPQMLIAPINGPTDEVARGRFQRALLDKYPNVSVIDVADILRAVTRILNNVTLAV
                     SFIGGFVLLSGVLILVGSIAMTKWQRIYEAAVLKTLGAKRKVLLAIMLAEYGLLGLTA
                     GTVGTLAAVALSYALSRYVFELPWSFSPWLYLAGLGATVALVATVGALSSLDALTRKP
                     LAVLRAP"
BASE COUNT          897 a         1409 c         1480 g          920 t
ORIGIN      
        1 cgtcagccgt gtaatcgtgt gcccgccatg tcggttattg cgcatcgggc acgacttcta
       61 tcatgcgata gaagtgtgta gcaactgaaa gtcttgcacc gaaggtgcat agttttgcac
      121 tagcgtgtct gtgacaataa acgtgtgtga tgggttggtc ggggtgatag atgacttctc
      181 ccgccgacag gatgacgggt tccagatcgg gtgcgaggcg ttcgtgttcg gcgcgggtta
      241 aggcgttgag gatgcggttc gaggttgaag gatgtaattc cggaagcgtg gacattggta
      301 gcctttctgt gtacggtgcg taaaagacgg cacgcggcag aaagtgagcg taggggctaa
      361 cttaatgtcg aatggagctt gtcaaaaggg taattcctag tgcgggagaa gtatctcaca
      421 ctttgtacga aatggacagt cgctatgtcc gataccgcac atagccggat ggcggaggtc
      481 tttgaagttg agttctgcga cgattgaggg gcagacttac gccctgttcg ccctgtccgc
      541 cctgcgcgcc gaacggctaa tacttcttca caatcgaggg aagccgctgg cgactgctgc
      601 ctgaggtcag gccgcacgac ttttgaagca cgaacgggcg cggtctatct aagaccgcgc
      661 ccgttcaaag ccgcgctaac ttttgtaacg ccctcttttt gtaaccgccg ttcttttcag
      721 acaggcaacg tgccccgtct ctctatcctt aacgcgcgcc gaggccgccg cgcgactgca
      781 agcccatcag gcgcgcgggg aacacgccga agtcttcgac ctcccaggtg ttgccgtctc
      841 tgagcctgac tttggcggcg gggatcaacg tcccttcgct gttgcggcgg cgcatgaaga
      901 tttccgcgta gccgaacttg taatcggtcg agcgcgcccc ggcgcgcgcc tcgtagatgt
      961 tgatgttgcg ctcgtagaga acgatcagtt tcgtcccgtt ctcatgcggc acgctgatga
     1021 tcgcgttggc atccacgccg acgccgttcc cgacagtgat tcttccggcg tccatccgcg
     1081 agagcacgcg cagcagttcc tcttgccccc ccgattgcag cgcggcgttc aattgccgca
     1141 cctcttcatc cgacgtgtag cggttgatga tcaggtcgaa cgaagtcgtg cgcccggcga
     1201 accgtccgcc gacgccgacg accgttccgg tgatgtgctg ccgcccacct tcgaccgccc
     1261 ggctgcccct gcgctgcccg aacgcgaccg gcgcaggctg cgccagcatc aatcccaaca
     1321 cgcaacacgc cgctgccaac caacccgaca ctcttctttt ctccataact tccccctggt
     1381 gaaatccgaa tttacttttt acccgctaac actaaaccgc agaagtgtac agagtgtctc
     1441 aaatttctct caagttgtgc cgcatcaagg atattcgccc aaagtgcgtg tgattcccgt
     1501 cccgcacgtc gaagcgtggg cgtcgtcagg ggcaactctc gaacgcgcgg caagccgaaa
     1561 cgataagcgg cgcaaaagag acggagagag agtcgagttc gacgttgaac tcgactctct
     1621 ctccatccgt gcttccggct ccggcggact cttccttcgc gcccgcaggg agactaccgg
     1681 cgtcaatgag aagcttgagc gggcggctgg gcggggcggg cgttgatgcc tgtgatcggg
     1741 tagggttctt ccgtgccgac ggtgaactcg aaggcgagcc agcggccatc ttttttcgtc
     1801 tgcacgagca aggcgatctt gtctttcgtc tcgctcggcg aaaggctgtg gagtttgagg
     1861 ccgcccgtcc gctcgtagac ttcgaagtcc ttgtcggcgt tctccgggtt gccgctcgtg
     1921 ctttcgtgga aggcttgcat ctgtttgaga tcgccgctgt tgaaggcggc gaagaatttg
     1981 gcgacggtct ttccggcggg cgttttggca agctcttctt cgggcgtcgt cgaggcggtc
     2041 gccgttgccg tagcgggctt atctttgccg agcgcggcgg gggcggcttt ctccgtcttg
     2101 ttcgcggggt tgcctgcggc gggggtttgc gcggggcagg cggcgacggc gaagaggccg
     2161 aggacaaacg cgagcgagag gcgtttgcag cgtgctgtca gttgatacat gattctttct
     2221 ccaggttgat gactcgttaa actcacgcac agtatcgctg aaacgccgac gcggttggtt
     2281 aagaggatga aaaaagattg ttaaggatgt aaaaaagcag tggcaagaga caggtgacga
     2341 gggacaagtt aaaaagacgt aaatcgtgaa tcgtgaatag agaaaacctg tcttcgttga
     2401 ttcacgattc acgatttact tgtcttttct cgtcgcttgt cactcgtcac tcgtcactgt
     2461 aattaaggtg cgcggaggac ggcgaggggc ttgcgcgtga gcgcgtcgag acttgagagc
     2521 gcgccgaccg tggcgacgag cgcgacggtt gcgcccaagc ccgcgaggta gagccacggc
     2581 gagaaactcc agggcagttc gaagacgtag cgcgagagcg cgtaggagag cgcgacggcg
     2641 gcgagcgtgc cgaccgtgcc cgccgtcagc ccgagcaggc cgtactccgc gagcatgatg
     2701 gcgagcagca ctttgcgttt cgcgccgagc gtctttaaga ccgccgcctc gtagatgcgc
     2761 tgccatttcg tcatcgcaat cgagccgacg aggatgagca cgccgctcaa gagcacaaag
     2821 ccgccgatga aggagacggc gagcgtcacg ttgttcaaaa tgcgcgtgac ggcgcgcaga
     2881 atgtcggcca cgtcgatcac ggagacgttc ggatatttgt cgaggagcgc gcgttggaaa
     2941 cgcccgcgcg caacttcatc cgtcgggccg ttgatcggcg cgatcaacat ctgcggcgcg
     3001 ttctccaagg cgcccggacg gaagaggatg agaaagcccg tgcgcgagtt gcgccagtca
     3061 acgcggcgga tgcttgtgac gcgcgccgtc agacgccgac ccgcgatgtc gaaagtgatc
     3121 gtcccgccca gttccaaccc cgcgaggccg cgcatgccct cttcgatgga tatttccggc
     3181 tcgctcgaag gagcggcgtc ccagaatttc ccggccacaa tcgtctcgtt cgcttcgagt
     3241 tgcggcctgt aggtgacgac gtattcgcgc gcgaggaggc cgcgttgcgc gcgtcgccct
     3301 cggtcgtcca catcgatgct ctgcccgttg atggctgcga tgcgcgtgcg caccgtcggg
     3361 acgagcgtgg ggcgttcgcc cgtcgcctgc gtgatgagtt cttcgacacc gtttttctgg
     3421 tctgtctgca cgtcgatcag gaacatgttc ggcaactgtg cgcgcgccgc cggatcaaac
     3481 tcacgcacga gattcctttg cagcgattgc gtggcgatga cgaggaacgc gccgaggccg
     3541 acggccagca ccaccacgcg cgtctgattg cccggacgat acaggctatt gatcgcgtgc
     3601 cgcagcggga acgagcgtat gtgccgcgca cgccgtacga ggaagatcac gagcatcgcc
     3661 gtcaaattga gcacgaacgc cgtcgcggcc aagcccgcca tgaagaacgc gcccacgcga
     3721 atcgatccgg cctgccacgc ggcgagcaac accaggccgc ccaccacgac gaacgccacg
     3781 agcgagcgag ccgccccgat gcgcctgaga gactgtagcg cgtgacgggg agacaagaag
     3841 cggcggagtc tcagccacca accacgccag cccgcttcgg gttgcgcctc gtcgtcgcta
     3901 ccgttgttgt cgccgcccgc agcgtcttcg cgcagcagca tgttcggttt gatgcgacgc
     3961 acgcgcagga gcggcagcgc ggagaagagc agcgagatga gcaggccgag catcaagcct
     4021 tgcgcgattg cgccccacgt caactcgtag ctcatgttgg ggggcagcgt ctctgcgaat
     4081 cgccgcgaga cgaagagcag agccgcacgc gcgagcacga cgccgaagag acttccggcc
     4141 agaccgagcg cgagcacctg cgccagatag acgcccgtga tcacgcggct cgacgcgccc
     4201 acgcatttga gcacggcgat ggtgcgcttc ttctgttcga taaaaacgcg cgttacgttt
     4261 gagacgccga tgccgccgag cacgaggatg acgaggccgg tgagcgaaag ataattttcc
     4321 gagcgcgtgt attgctcgtt caagccctcc tgcgaatccc tgtaggagcg cacgctgacg
     4381 aggttctcct tcaagcccgc gcgcaactcc gcgacgagcg cgttcaactc agcgggcgga
     4441 actttcagga gaacccggtt gcgggcgcgg ctgccgaatc cggttaagcc cgtctgtgca
     4501 atcgcttcgc gcgcgatgaa gacgcgtgga ccaatgcgaa agccgcccgt cgcgcccggc
     4561 tcctgtccta tcgccccgcg tatctgaaag gtcgaattcc cgatcttgac ttcatccccg
     4621 acactgaggc gcagacgctc cagaagcgac gcggcgacca ccacaccgtt gttcgcaagg
     4681 agcgcgtgtg tgaacctctc gccgct
//
LOCUS       DASWNS010000518         6704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_253266, whole genome shotgun sequence.
ACCESSION   DASWNS010000518 DASWNS010000000
VERSION     DASWNS010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253266"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..835)
                     /gene="rimI"
                     /locus_tag="VGB76_17855"
     CDS             complement(326..835)
                     /gene="rimI"
                     /locus_tag="VGB76_17855"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY0100816.1"
                     /translation="MSQVELVTERIQSDFQIERMTAHDLLEVVEIEEACRLSLWGWDS
                     YHAELLRPEAIMLVARRVRADDLNGHSLHGFLSARVSASELHINNIGVHETARRRGVG
                     NALMSVAFDAGRRQGALTSILEVRAGNAAAQSLYRRFGFAVVGRRRQYYRDPVEDALL
                     MNASLKPPT"
     gene            complement(823..1653)
                     /gene="tsaB"
                     /locus_tag="VGB76_17860"
     CDS             complement(823..1653)
                     /gene="tsaB"
                     /locus_tag="VGB76_17860"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY0100817.1"
                     /translation="MRSNSKTSLRREVSAGDSLKDAQAAPLILSLDTATATRSVAVVQ
                     GERVLARLGGRVQKENSSSVLQDIDEALATSGAKLKEIELFAVTTGPGSFTGLRSGIA
                     TIKAFAATLDRPVSGVPTLHAIASAGATARRVLASLPAGRGEVFAQLLSVDGEGLVKE
                     LSEPFHLSPAALLEQAVGWGGGLTWAGAGAHAHAAAIREFAGEQKFVWRDEPEAEDES
                     GREQREVWTLAPLTEFYAERVAALGLINYRQGSTIDAENLHALYVRLSDAELNERCRR
                     "
     gene            1753..2790
                     /locus_tag="VGB76_17865"
     CDS             1753..2790
                     /locus_tag="VGB76_17865"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY0100818.1"
                     /translation="MSVRIGILTAGGDCPGLNAVIRAVVRRALVEDAPVFGIKNGWRG
                     LIEDDIIPLTRSSVSGILPRGGTILGTSRTNPFDDAKSLEQIRHNWEKHKLEGLIVVG
                     GDGTLRTARDMWREHNYPIVGIPKTIDNDVGGTDFTFGFDTAVSIVTDAIDRLHSTAE
                     SHHRVMVVEVMGRHTGWIAAYGGIAGGADVVLVPEHPFRLSRVCDFLRQRAAQGSAFS
                     IIVVAEDARPVPEENFLTEEVRQQIYRQGRLGGIGIALAQEIERVSGIETRFTQLGYV
                     QRGGSPTAFDRVLATRFGIKAFDMVAAGEWGQMAALHGKRVVSFPLDEALREVKKLSD
                     EIYHVAELFFG"
     gene            2870..4864
                     /locus_tag="VGB76_17870"
     CDS             2870..4864
                     /locus_tag="VGB76_17870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0100819.1"
                     /translation="MKLCRTCRRCYEDTDAVCEIAEHGGLSHARPGSRLIAEKYRLDS
                     LLGRGGMGAVYSGSHVELERPVAVKMLLPDSVSDPQALERFRREARAAARLNHPNVAN
                     TYDYGALADGEAYLIMELLDGQTLREYLNAEGSLPLKWAVEIARQVGDGIGAAHHSEI
                     VHRDLKPSNIILTRDHRGELQAKVLDFGIARILEQTTTSGDPLTAAGALIGTPRYMSP
                     EQCAGHDSDARSDVYSLAVILFEMLAGRPPFDAPSATAIALKHVREMPPDILQLRPGV
                     PPALARLISESLAKDPSARPQTATEFASRLHEIGEALAPDDHSISIFDVAADGKLSTT
                     SEIQGAAATSAVPQEFQTVAVEDAAPHTDLHPDERPTDRVGEPTFGDLISDDPASSEE
                     EVEAEVSPQTAPQSVSQTWQPAQVTPPPDSLHASAATRASNAPRDETETVFSSSPPGS
                     LEGESAGRRFPLALVIGSFALAALLVALLYAWRGRTTDQVAVTNPDAAPSPVATPSAS
                     TEATPESSPSAAPSSSPPQPAVAVDAPVAAEKERAALRSTVDSWLAAHNTRNLSQLTN
                     FYMRNVSAFYRTQNTSRAAVRAEKARLFKESALSIKRTSDPQITLAPDGRTATMLFNK
                     SYSRGAAGNADSGEVLQEIVWQKTNRGWKIISERDLRVIR"
     gene            4976..>6704
                     /gene="mutS"
                     /locus_tag="VGB76_17875"
     CDS             4976..>6704
                     /gene="mutS"
                     /locus_tag="VGB76_17875"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY0100820.1"
                     /translation="MAEPTLTPMLRQYHELKRQHPGTILFFRLGDFYELFFDDAVTGA
                     RELEITLTARHKERGGAGIPMCGVPHHSLSGYISKLIRKGYRVAICEQTEDPTKTKKL
                     VRREVVRVITPGTAIDPQLVESRDCVYLAAVCGAGDSYGAAFLDLSTGEFRTTEARGA
                     DAWPRIRADIESYSPRELLFPASLTPLIKNAYAAGGASQTEPLPPASDRDTNVVPFAR
                     PAHAETANFGGACMSLSPLDDWLWEPISCASLLLEQFGVRTLEGYGLDGKIDAVAAAG
                     ACLRYACETQRDAAAHVNDITYFESQDHLVLDAVSVRNLELVEAQNNAGRSHTLFGVV
                     DSTVTGMGARLLRAWLLRPSVRRGEIEARLAAVEDFHRSHMKRDRMQALLKDVADLER
                     LIARISMNTATPRDLAALRRSLEQVPTLRQTLSDSASSLLQVLRENADELPDVRELIA
                     QAISDDPPLNLSDGGAIRANYSAELDELRDISRNAKQTIAALEARERTRSGINSLRVR
                     FNNVFGYFIEVSKGQAARVPSDYERRQTLANAERYTTPELKEWEQKVMGAEGRIGELE
                     ATIFAEVRAR"
BASE COUNT         1292 a         2174 c         1956 g         1282 t
ORIGIN      
        1 tgcatctgat ctttcacggc cagcttcttt ttcttcagag tgatctcttc caactgctcc
       61 tcgtcgttcg ggtaagcgag ggaagttagt tcgctgagtc gcgcttcgta gcgtccgtgc
      121 tcgcgcgcca gttcgcgaaa ctcgggatcg ttcataatta attcttctct caagctgtcc
      181 gtgttggtaa gttccatctc agggaagcct cctttgtaga tgcgctttaa gtggtgtgca
      241 tgattcgcgt tcgtggcaag tcgtggtcgc ccttcccgcg tctattattt cgatgggaaa
      301 atggtagcta aaaacctcgc ctgaatcaag tcggaggttt cagcgaggca ttcatcagca
      361 gcgcgtcctc aaccggatcg cgatagtact gccgacgcct gcccaccact gcaaacccga
      421 agcgacggta gagagactgc gccgccgcgt tccccgcgcg cacttcgaga atagacgtga
      481 gcgcgccttg ccgtctgccc gcgtcgaagg ctacggacat caaagcgttt cccacgccgc
      541 gccgccgtgc ggtttcgtgg acgccgatgt tgttgatgtg cagttcgctt gcagagacac
      601 gcgcgctcag aaagccatgc agagaatgtc cgtttaagtc gtcggcgcgg acgcgtcgag
      661 ccacgagcat gatcgcttcc ggacgcaata gttcggcatg atatgaatcc caaccccaga
      721 ggctcaggcg acacgcttct tcgatctcga ccacttcgag cagatcgtgc gccgtcattc
      781 gctcaatttg aaaatcgctc tgtatccgtt cggtgacaag ttctacctgc gacatctctc
      841 attcaactcc gcatctgaca aacgcacata gagcgcatgt aaattttctg cgtctatcgt
      901 acttccctgc cggtaattta ttaaaccaag cgcggcgacc cgttccgcat aaaactctgt
      961 gagtggcgcg agcgtccaaa cttctctctg ctctctcccc gactcgtctt ctgcctccgg
     1021 ttcatctcgc cagacaaact tctgctcccc tgcaaactcc cgtatcgccg ccgcgtgtgc
     1081 gtgcgctccg gctccagccc acgtcaggcc gccgccccac cccacagcct gctccaacaa
     1141 cgccgccgga ctgagatgaa acggttcgct caactccttg accaagcctt ccccgtcaac
     1201 cgatagcaac tgcgcgaaga cctctccgcg cccggcgggc aacgaagcca gaacgcgccg
     1261 cgctgtcgcg cccgcggaag cgatggcatg gagcgtcggc acgccggaga caggccggtc
     1321 tagagtcgcg gcaaaggctt tgatggtcgc gatgcccgat ctcaatccgg taaaacttcc
     1381 gggtcccgtt gtcaccgcga agagttcgat ctcttttaac ttcgcgcccg atgtagccag
     1441 agcctcgtct atatcttgca gcacggacga ggaattttct ttctgcacgc gcccgccgag
     1501 gcgagccagg acacgttcgc cctgcacgac ggcgacgctt ctcgtggcgg tcgccgtgtc
     1561 gaggctgaga atgagcggag cagcctgtgc gtccttcaaa gaatcacccg ccgacacctc
     1621 tcgccgcaac gaagtcttac tattgcttct catgtttgac atgcactggt atgccgcgcg
     1681 attataggcg cggctttcaa ttcaagcaaa taagggtggc tcacggttga gctgtttcac
     1741 tcggttgtac tgatgagtgt ccgcataggg attctgacgg cgggtggcga ttgccccggt
     1801 cttaacgctg tgatccgcgc tgtcgtgcgt cgcgcactgg ttgaagacgc gccggtcttc
     1861 ggcatcaaaa acggctggcg cggcctgatc gaagacgaca tcatccctct cacgcgcagt
     1921 tccgtctcag gcattctccc gcgcggcggc acgattctcg gcacatctcg caccaacccg
     1981 ttcgacgatg cgaaatcgct cgaacaaatt cgccacaact gggagaagca caaactcgaa
     2041 gggctgatcg tcgtcggcgg cgacgggacg ctgcgcacgg cgcgcgacat gtggcgcgag
     2101 cacaactatc cgattgtcgg cattcccaaa actatcgata acgatgtcgg cgggacggat
     2161 tttactttcg ggttcgacac ggcggtcagc atcgtcacgg acgcgattga ccggcttcat
     2221 tcgacggcgg aatcgcatca tcgagtgatg gtcgtcgaag tgatgggacg ccataccggg
     2281 tggatcgccg cctacggagg catcgcgggc ggcgcagacg tggtgctcgt ccccgaacat
     2341 cctttccgcc tctcgcgcgt ctgtgatttt ctgcgccaac gggcggcgca gggcagcgcg
     2401 ttttcgatca tcgtcgtggc ggaagatgcg cgccccgtgc cggaagagaa ttttttaacg
     2461 gaagaggtgc gccagcagat ttaccgtcag gggcggcttg gcggcatcgg catcgcgctg
     2521 gcacaagaga tcgagcgcgt ctccggcatc gaaacacgct tcacgcaact cggctacgtc
     2581 cagcgcggcg gctcgcccac ggctttcgac cgcgtcctcg ccacgcgttt cggcatcaag
     2641 gctttcgata tggtggccgc gggcgaatgg ggacagatgg ctgcgctgca cggcaaacgc
     2701 gtcgttagtt tcccgctcga cgaagccctg cgggaagtca aaaaactgag cgatgagatt
     2761 taccacgtcg cagaactatt cttcggctag ttcaggccgg acgcggattt gagttaatac
     2821 gccgcgccgg gcttgaacct ttgtcaaaac gtttggagcg tttggtcgga tgaaactctg
     2881 ccgcacatgc aggcgttgct acgaagacac ggacgcggtc tgtgaaatcg cggagcacgg
     2941 gggactgtcg cacgcgcgcc ccggctcacg cctgatcgcg gaaaaatatc gactcgacag
     3001 tctcctcggt cgcggcggga tgggcgcggt ctattccggc tcacacgtcg aactcgaacg
     3061 tcccgtggcc gtcaaaatgc tcctgcccga ttccgtctcc gacccgcagg cgctcgaacg
     3121 ctttcgccgc gaagcacgcg ccgccgcacg tctcaatcat ccgaacgtgg cgaacactta
     3181 cgactatggc gcgctcgcgg atggtgaggc ttacctcatc atggagttgc tcgacgggca
     3241 gacgctccgc gaatatttga atgcggaggg cagccttccg ctcaagtggg cggtggagat
     3301 cgcgcggcag gtgggcgacg gcatcggcgc ggcgcatcat tcggagatcg tccatcgcga
     3361 cctgaagcct tcgaacatta tcctgacgcg cgatcatcga ggcgaactac aagccaaggt
     3421 tttagacttc ggcatcgcgc gcatccttga gcagacgacg acgagcggcg accctttgac
     3481 ggctgccggt gcgctcatcg gcacgccgcg ttacatgtcg cccgaacagt gtgccgggca
     3541 tgattcggat gcgcgctcgg atgtctatag tctggccgtc atcctcttcg agatgctggc
     3601 cggtcgcccg cctttcgacg cgccctcggc taccgcaatt gccctcaagc atgtgcgcga
     3661 gatgccgccg gatattctcc aacttcgtcc tggcgtgccg cccgcgctcg cccggctcat
     3721 ctccgaatcg cttgccaaag acccgtctgc gcgcccgcaa accgccaccg aatttgcttc
     3781 gcgattgcac gagatcggcg aagccttagc gcctgacgac cactcgatct ccatatttga
     3841 tgttgcggcg gacggaaaac tttccaccac ttccgagata cagggggcgg cggccacgag
     3901 cgccgtgccg caggagtttc agacagtcgc ggttgaagac gcggctccgc acacagacct
     3961 ccatccggac gaaaggccga cggatcgcgt gggagaaccg accttcggcg atctcatctc
     4021 cgacgatccg gcgtcaagcg aagaagaagt cgaagcagaa gtttctccgc aaactgcgcc
     4081 gcagagcgtc tcgcagacgt ggcagcccgc gcaggtcacg cctcccccgg acagtctgca
     4141 cgcgtcagcc gcgacgcggg cgtccaatgc gccgcgagat gaaacggaga cggtctttag
     4201 ttcatcgcct ccgggttcgc ttgaaggaga gagcgcggga cgccgtttcc cgctcgcgct
     4261 cgtgatcggc tcttttgcgc tcgccgcgct cctcgtcgcg ctgctctacg cgtggcgtgg
     4321 gaggacgacg gatcaagtcg cggtcactaa tccagacgcc gcgccgtcgc ccgttgcgac
     4381 tccgagcgcg agcacagaag cgacgcccga gtcgtcgcct tcggctgcgc cgagttcgtc
     4441 gccaccgcaa ccggcagtcg ccgtagacgc gccggtcgcg gcggagaaag agcgcgccgc
     4501 gttgcgttcg acggtcgata gctggctggc cgcgcacaac acgcgcaacc tctcgcagtt
     4561 gaccaacttt tatatgcgca acgtctcggc cttctatcgc acgcaaaaca cttcacgcgc
     4621 ggcggtgcgc gccgagaagg cgcggctgtt taaagaatcg gccttgtcca tcaagcgcac
     4681 gagcgacccg caaatcacac tcgccccgga tggccgcacc gccacgatgc tctttaacaa
     4741 gtcctacagt cgcggcgcgg cgggcaatgc cgacagcggc gaagtcttac aggagatcgt
     4801 ctggcagaag acgaatcgcg gttggaagat catcagcgag cgcgacctgc gcgtgattcg
     4861 ctgaaagaaa agcagaattc cgcctcttat tttcgcgctc cccggtagcg acttgcccac
     4921 cgtcaattga tccaatacat tatcgcttac gaagcgtttc ctctctggat gcaacatggc
     4981 tgaaccgaca ctgacaccaa tgctgcggca gtatcacgaa ctgaagcggc agcaccccgg
     5041 cacgatcctc ttttttcgtc tcggcgattt ctacgaactc ttcttcgacg atgccgtgac
     5101 cggcgcgcgc gaactcgaaa tcacgctcac ggcgcgccat aaagagcgcg gcggcgcggg
     5161 catcccgatg tgcggcgtgc cgcatcattc cctctccggc tacatctcca agttgattcg
     5221 caaaggttat cgcgtcgcca tctgcgaaca gaccgaagac cctacgaaga cgaagaaact
     5281 cgtccgccgc gaagtggtgc gcgtcatcac gccggggacg gcgattgatc cgcagttggt
     5341 cgaatcgcgc gattgcgtct atctggcagc cgtgtgcggt gcgggcgact cctacggcgc
     5401 ggcttttctc gatctctcga ccggagaatt tcgcaccacc gaagcgcgcg gcgcagatgc
     5461 ctggccgcgc atccgcgccg acatcgaatc ctacagcccg cgcgaactcc tcttccccgc
     5521 ttcgctcaca ccgctcatca aaaatgctta cgccgctggc ggcgcgtcgc agacagagcc
     5581 tctgccaccc gccagcgaca gggacacgaa cgtcgtcccc ttcgcacgcc ccgcgcacgc
     5641 cgagacggca aacttcggcg gggcttgcat gtctctcagc ccgctcgacg attggctctg
     5701 ggaaccgatc tcctgcgcct ccctgctgct cgaacagttt ggcgtgcgca cgctcgaagg
     5761 ctacgggttg gacgggaaga ttgacgcggt agcggcggcg ggcgcgtgcc tgcgctatgc
     5821 atgcgagacg cagagagacg ccgccgcaca cgtcaacgac atcacctatt tcgagtcgca
     5881 ggaccatctc gtgctcgacg ccgtgagcgt gcgcaatctc gaactcgtcg aagcgcagaa
     5941 caacgccgga cgctcgcaca ctctgttcgg cgtggttgat tctaccgtca cgggcatggg
     6001 cgcgcgcctg ctccgcgcgt ggctcttgcg cccttcggtg cgtcgcggcg agatcgaagc
     6061 gcggctggcg gcggtcgaag attttcaccg ctcgcacatg aagcgagatc ggatgcaagc
     6121 gctcctgaag gacgtggccg acctcgaacg cctcatcgca cgcatcagca tgaacacggc
     6181 cacgccacgc gatctagccg ccctgcgccg ttcgctcgaa caggtgccga cgctgcgaca
     6241 gacgttatcg gactccgcat cgtcgctcct gcaagtcctg cgtgaaaatg cggacgagtt
     6301 gccggacgtg cgcgagctaa tcgcgcaggc catcagcgac gacccgccgc tcaacctctc
     6361 ggacggcggc gcgattcgcg cgaactattc ggcggaactc gacgaactac gagacatcag
     6421 ccgcaacgcc aagcagacca tcgccgcact cgaagcgcgc gagcgcacgc gctccggcat
     6481 caattcgctg cgcgtgcgtt tcaacaacgt cttcggctac ttcatcgaag tctccaaagg
     6541 acaggccgcg cgcgtgccct ccgattacga acggcggcag acgctcgcca acgccgaacg
     6601 ctacaccacg cccgaactca aagagtggga acagaaagtg atgggcgcgg aagggcgcat
     6661 cggcgaactc gaagccacca tctttgcgga ggtgcgcgca cgcg
//
LOCUS       DASWNS010000519        11577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_460142, whole genome shotgun sequence.
ACCESSION   DASWNS010000519 DASWNS010000000
VERSION     DASWNS010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11577
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460142"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..564)
                     /locus_tag="VGB76_17880"
     CDS             complement(163..564)
                     /locus_tag="VGB76_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100821.1"
                     /translation="MDRTRQLLFILLLLVGWSFSGALTGKLLSVPQTRAEETEQSNAG
                     MKWEYCALSKAAYAGSARGGIYWISYFQETGVQVVEVQASVTEGNGAAMAKAISKLGS
                     EGWEMVAPAPLEVNQVSTTALYFKRPKAAAR"
     gene            1192..4128
                     /locus_tag="VGB76_17885"
     CDS             1192..4128
                     /locus_tag="VGB76_17885"
                     /inference="COORDINATES: protein motif:HMM:NF036537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LodA/GoxA family CTQ-dependent oxidase"
                     /protein_id="HEY0100822.1"
                     /translation="MATKKKQGNESESVGWNCADDPAGKLKQMFVDIVQTRRIEQGQS
                     PARRPVFLKLHGVAHGRFEMRADLPAELRVGVFALKSLPAWVRFSSDTHPTNPDLKTT
                     CGIGIKLFEVPGEKLLGDGRTQDFVMQNHDVFFVDTAQDMCEFTKAGVVDGDYTPYLE
                     AHPVTKKILDEMEKVELSVLTTTYWSVLPYSFGKGRFVKYKLEPEQVPDGEPPTDDPN
                     YMASDLARRLRSGDARFKFMVQLRTKPKTMPLDEATVRWSEEESAPIHVATLILPKQD
                     VSALGQAAYGENLAFNPWHSLPEHKPQGSISAARRVVYATSADERRNVNGVTTREPDE
                     AKPVSVLPQPVDRTIVKAAIHPSIGVARVGNSMDEFFIGPEVTDPLPETPGFYRDKTG
                     ALKRQAARFRVYGLNAEGQVVAELNAQNATVEWTAHLANKKSAWYQFQLALDIPEAAD
                     APPSLLRNAGVADRAQLVIDPGQRHAKGVNQQGGKRHLFDTGEFMGTPVYLGELRTDE
                     AGRLIVLGGRGVSASFDGSKAVTFANNEGWHDDVSDGPVTAKVTFEGQKLKVDPAWVV
                     VAPPNYAPMQKSVRTMWDLIRDTAIQAGMLPQPARPSFEQDIRPLFERMSRLQWVNAG
                     FAAAFGWNSPSDFSRPELLAQLADPSPNAKELRQTVANHFRNFERDSWSPTPWPWLYG
                     DAMNVPPAETPRQHVALTDTQQGFLQQWVRGDFEADYDPQRQPPHRIEEVPVAEQAAM
                     LDKAALDDCLADAFHPGCELTWPMRHSTLYMSAFRIEHAPRNWKEPSYGGGLIQDTLS
                     LPEGPLAAQVPGGLTRWMALPWQTDSASCRSGYLKTYDPYLPTFWPARVPNQIMSREN
                     YEIVMDESRPLGERLAAFANRAAWINTLGSVSYTEQINNMIKHFGEMGVVEVREGPKD
                     DEHFPPVMEVAQHPTRRQRGLLKATEAEAAERPRLLDQHKFLGDADVELNGIEKVRRF
                     PYGLRR"
     gene            4142..5227
                     /locus_tag="VGB76_17890"
     CDS             4142..5227
                     /locus_tag="VGB76_17890"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY0100823.1"
                     /translation="MLEADALIVGAGPAGCAAAINLAPLRQVLLVERRAEVTPRVGES
                     LVPAARRLLTDMGLWESFIAEGHEPWYGNRAVWGAPEPLEVDFMRDPDGHGWHLDRAR
                     FEQWLRAHATKRGAGFIAPASVDHLAHDGERWQVQLNTPASQCTANVKLIIEAGGRAA
                     PVARKQGARVQASDRLICAWVSGRDARESNRGLTYVEAVEDGWWYTAPVPGRQRILAF
                     YTDADLPNARVINKRDGLLERAASAEELSALLTAAGFVPEAQSHVTAAHSAMLDPCAE
                     ESWLAVGDAAISFDPLSSQGLLNALFTGLAAANAADRYLEGDAHSLSVYRQVVRGIGD
                     AYRQHLASFYATEQRWPEAQFWRRRRA"
     gene            5504..6346
                     /locus_tag="VGB76_17895"
     CDS             5504..6346
                     /locus_tag="VGB76_17895"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0100824.1"
                     /translation="MSKVWFITGAGRGMGIDIAKAALAAGYKVVATGRNTDMVTQAVG
                     ESAGLLAVKLDITNPADAEAAVKAAVDRFGRIDVLVNNAGNFYAGFFEEITPEDFRAQ
                     IETNLFGPLNVTRAVLPVMRQQRSGLVVTISSIAGIVGQEFVSAYCASKFGIEGWMES
                     LTPEVAPFGIRTMLVEPGFFRTELLTEESTKYPEPSIDDYAEKTGQTVTAWKGMNGLQ
                     GGDPAKLAKALIQLASQDEPPLRWAAGADAVQAVEQKAKDLLAQADAYRELSSSLAHD
                     NTSA"
     gene            complement(6487..6735)
                     /locus_tag="VGB76_17900"
     CDS             complement(6487..6735)
                     /locus_tag="VGB76_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100825.1"
                     /translation="MTDEERQRQMDFILDSLARLTATTSQLAESHARDHARIARLEES
                     FVILTDLAGSYGERLGGVEEAVAELRRLSGSEDGGLQP"
     gene            complement(6985..9690)
                     /locus_tag="VGB76_17905"
     CDS             complement(6985..9690)
                     /locus_tag="VGB76_17905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0100826.1"
                     /translation="MPDWKDEIRRLLSELKLAPTREAEIVEELAQHLDDRFEQLLQGG
                     ATREEAYRAALLELNESNLLAHELRRVERPVQQEPVVLGARGRTNIMTDLWQDLRYGL
                     RMMLKNPGFTVVAVLALALGIGANSAIFSVVNTVLLRPLPYKDPERLVMVWEDDTKGG
                     YPRDTPAAANYVDWRDRNGVFEGMAAIAEQNFNLTGTGEPEKIEGRRVSANLFSLLGV
                     EPQVGRAFLPEEDQPGRNRVVVISYGLWQRRFGGDAKIIDQSLTLNGESYTVVGVMPP
                     HFQFPRREDELWVPIAFTPQEAANRGRHYLEVVARIKPGVTLEQAQAEMSTIAAQLQQ
                     QYPEQNTDLGVAVTPLHEQVVGDIKPALLVLLGAVGFVLLIACANVANLLLARAAVRQ
                     KEISIRVALGASRLRLVRQFLTESVLLAVLGGVVGLLLSLWGVSLLKAFIPENISQVE
                     AITVDLRVLGFTLLVSLLTGLIFGLAPAMQASSLNLNETLKEGGRDSAAGSRGNRIRN
                     LLVITEVAVSLIMLIGAGLLINSFLRLRNVDPGFRAENLLTMQVELPQLKYPQHAQRT
                     AFYNELLSRIEALPGVKSAAVTTNLPLYMQGNSIGISIEGIPDPAPGQGKRPVVATRV
                     VSPHYFQTMSIALLQGREFSEQDKFDSPAVAVINETMARRYWPEQDPTGRRITSGAPT
                     STDPDDWITVVGVVKDVRQFELVTDPKPQMYLSYAQAGFFAPNDLVVRTEVEPLSLAA
                     TVRRKVWEVDKDQPVSKIRTMEEIVSESVARQRFSMLLLGIFAALALVLAAVGIYGVM
                     SYSVAQRTREFGIRMALGAQRRDVLKLAVGQGLKLVIAGIVLGLGASFLLTRVMSSLL
                     FNVSATDPFTFITISLVLIGVAVLASFIPALRATKIDPMDALRYE"
     gene            complement(9683..10036)
                     /locus_tag="VGB76_17910"
     CDS             complement(9683..10036)
                     /locus_tag="VGB76_17910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0100827.1"
                     /translation="MSYIGSNMLDRELKKGSAELLILSLVEDQPRHGYDIGNLIEQRS
                     RGLLRFNVASLYPLLYRLENRGWIQGRWVEKAGQRRRRYYRLTPAGKKVLAAQLVSWR
                     EFVEAINRIVGIEHA"
     gene            complement(10133..>11577)
                     /locus_tag="VGB76_17915"
     CDS             complement(10133..>11577)
                     /locus_tag="VGB76_17915"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEY0100828.1"
                     /translation="AALLHLFIRRSLLPLLERRFSPPTYDDRRILLDLSEAARAATNT
                     DQLYKYICEMIRDALRSENVSLFVREGASGEFVCRMSAEQHAPKPHAGAEAQSPAHEE
                     RAATPALRSDAFVVNRLRNLAIPLHIEPQDLETWVRALANAPPTLQAERAAECATLRS
                     LRSSLMLQIKMKDQLVGILSIGRKQSAQEFSPKDEQLLMSITGQLALVIENSRLIERM
                     VEEERLRRELMLAAEVQQRLFPAHPPTSDTLDLSGLCQPARGVGGDYYDFLTLDNGQT
                     GITVADVAGKGISAALLMSTVQASLRSQALANCASVSVAGSLANLVSAMNRLLCRSTG
                     IASYVTFFYAQFDAREGKLTYVNAGHNPPFLIRRREGAETCEPPVNDYLKLATGGAVI
                     GVFEHFGYEEETLDMQSGDLLFAYTDGVTEALNLAGEEYGEERLLVELAATTHATADE
                     IRDRVASRLREWCAGARQHDDLTLLVLKVK"
BASE COUNT         2308 a         3550 c         3490 g         2229 t
ORIGIN      
        1 gcggcgtgcg cagctctcct aaagaccatg cgcgcattct atcaacatca gctatcttgt
       61 gcgcgggcat cgaacccgcc cccaagatgg ttcattcatc cccaccgcaa gcggtggggc
      121 attaagaacc tttttcgtaa agttcaattc gcacccgcgc catcagcgcg ccgccgcttt
      181 cgggcgtttg aaatagagag ccgtggtcga gacttgatta acctccagcg gtgctggcgc
      241 aaccatctcc caaccctcgg aaccgagttt actgatcgcc ttagccatgg ccgcgccgtt
      301 gccctccgtg accgatgcct gtacttcgac gacctgcacg cccgtctcct ggaagtagct
      361 gatccagtag atgccgcctc tggccgaacc ggcgtaagcc gccttcgaca acgcgcagta
      421 ctcccacttc atcccggcat tcgactgctc tgtctcctcc gcacgtgtct gaggaacgga
      481 gaggagtttg cccgtgagcg cgccgctaaa tgaccacccg acgaggagca gtaagataaa
      541 gagaagttgt cgtgtcctgt ccataagacc tccgttcagt cgcgaggatt gtaggtgcgt
      601 tctaacgccg ttcatttaag acgctgcacc gtttcaacgc tcatgtggcg aaatgctgcg
      661 acacattcgc cgcttacaat gccgggcgcc tctgtgcagg gcgcgcgagg ccgtcatcta
      721 ctaacgcgaa atccgttaac taaagggatc atcccgaccg ccacctcctc tcagttcgcg
      781 ccggatagtt cggaacaggt taccgcccgg aactacccgg caccgggcaa gtcatcggcc
      841 tctaacgctg gtgtatgttt cgcccgcgca cctctatctt ctaaagtaag gtagatattg
      901 acacgggtgc ttaatggtca tatcgtgttc ctgtctccag gggtttatcg tttctcgcta
      961 acgagccgct ctaccttcgt tagtctcact tcaacaaacg gaactaaatc aaaattgccg
     1021 acgacgcagg ctcacgtgtg ttacgcacgc gcaggccacg ttgcggaggc tcttatcttt
     1081 ctcgcttcgc gattactgtc tcttcgtcta tgccagtgca ggctggcgcg ccagagcact
     1141 cgccgcgcgc ggggctgtct ggctgcgcac aatgcgaaag gaaactgact gatggctact
     1201 aagaaaaaac agggcaacga gagtgagagc gtgggctgga actgcgctga tgacccggcg
     1261 ggtaaattga aacagatgtt tgtggacatc gtgcagaccc gcagaatcga gcagggacag
     1321 tcgcccgcca gaaggccggt cttcctgaaa ctgcacggcg tagcccacgg tagattcgag
     1381 atgcgcgcgg acttgcccgc agagttgcgc gtcggcgtct tcgcgctcaa gagcctcccc
     1441 gcgtgggtgc gattttccag cgacacccac cccaccaacc ctgacttgaa gacgacctgc
     1501 ggcatcggca tcaagctgtt cgaggtgccg ggcgagaagt tgctcggcga cggacggacg
     1561 caggacttcg tcatgcaaaa tcatgacgtg ttctttgtgg acacggcgca ggacatgtgc
     1621 gagttcacga aggccggagt ggttgacggc gattacacac cgtatctcga agcccacccg
     1681 gtcacgaaga aaattctgga cgagatggag aaggtcgaac tcagcgtgct gaccacgacc
     1741 tactggagcg tgctccctta ttccttcggc aaagggcgtt tcgtcaaata caaacttgag
     1801 ccggaacagg tgcccgacgg ggaaccgccc acggacgatc ccaactacat ggcgagcgat
     1861 ctggcgcgca ggctgcgcag cggcgacgct cgcttcaagt tcatggtgca gttgcgcacg
     1921 aagccgaaga cgatgccgct cgacgaagcg accgtccgct ggagcgagga ggagagcgcg
     1981 cccattcacg tcgccactct gattcttccg aagcaggacg tgtctgcgct cggacaggcc
     2041 gcctacggtg aaaatctagc cttcaatccg tggcactcgc tgcccgagca taaaccgcag
     2101 ggcagcatct ccgccgcgcg cagagtcgtc tatgcgacca gcgcggacga acggcgaaac
     2161 gttaacggcg tgacgacgcg cgagcctgac gaggccaagc ccgtctccgt gttgccgcaa
     2221 cctgtggacc gcactatcgt caaggcggcg atccatccgt caatcggagt tgcgcgcgtc
     2281 ggcaacagca tggacgaatt ctttatcggt ccggaagtga cagaccctct gccggagact
     2341 cctggttttt accgcgacaa gacgggcgcg ctcaagcgtc aggcggcgcg cttccgcgtc
     2401 tacggtctga acgccgaagg gcaggttgtg gcggagttga acgcgcaaaa tgcgacagtc
     2461 gaatggacgg cgcatctggc taacaaaaaa tctgcgtggt atcaattcca gctcgcactc
     2521 gacattccgg aggccgccga cgcgcccccc tcgctgctgc gcaatgcggg cgtcgcagac
     2581 cgtgcgcagt tagtgattga tccgggtcaa cgacatgcca aaggcgtaaa ccagcagggc
     2641 ggcaagcggc atctcttcga cacgggcgag ttcatgggta cacccgtgta tctgggtgag
     2701 ttgaggactg acgaagcggg tcgtctgatc gtcttaggcg ggcggggcgt ctccgcctct
     2761 ttcgacggaa gcaaagccgt caccttcgcc aacaacgaag gttggcacga cgacgtttcg
     2821 gatggtccgg tcacggcgaa agtcactttt gaaggccaga aattgaaggt tgacccggcg
     2881 tgggtcgtgg tggctccgcc gaactacgct ccgatgcaaa agtctgtgcg tacgatgtgg
     2941 gacttgatac gcgacacggc gattcaggcc gggatgctcc cgcagcccgc gcgcccttca
     3001 ttcgaacagg acatccgccc gctcttcgag cgcatgtcgc ggctgcaatg ggtgaacgcc
     3061 ggtttcgccg ccgcgttcgg ctggaactcg ccgagcgatt tctcgcgccc ggagttgttg
     3121 gcacaactcg cagacccttc gccgaacgcc aaagaactgc ggcagaccgt cgccaaccac
     3181 tttcgcaatt tcgaacgcga ttcatggtcg cccacgccct ggccgtggct ctacggcgac
     3241 gccatgaatg tgcccccggc ggaaacgccg cgccagcacg tcgcgctgac agacacgcag
     3301 cagggatttc tgcaacagtg ggtcagaggc gatttcgagg ccgactacga tccgcagcgt
     3361 cagccgccac acaggattga agaggtgccc gtcgccgagc aggcggcgat gctggacaaa
     3421 gccgcgctgg acgactgcct cgcagatgcc ttccatccgg gctgcgaact gacatggccg
     3481 atgcgtcatt ccacgctcta catgtctgcg ttccgcatcg agcacgcccc gcgaaactgg
     3541 aaggagcctt catacggcgg cggactcatt caggatacgc tctctctacc ggagggtcca
     3601 ttggcggcac aggtgcccgg cggcctcaca cgctggatgg ctctgccctg gcagaccgac
     3661 tcggccagtt gccgttcggg ctacctgaaa acttacgatc cttatctccc caccttctgg
     3721 cctgcccgcg tgcccaatca gatcatgtcg cgcgaaaact atgaaatagt gatggacgag
     3781 tcgcgcccgc tcggcgaacg cctcgccgcc ttcgctaacc gcgccgcatg gatcaacacg
     3841 ctcggctccg tgagttacac agaacagatc aacaatatga tcaaacattt cggcgagatg
     3901 ggagtcgtcg aagtgcgcga gggaccaaag gacgacgaac acttcccgcc cgtgatggag
     3961 gtggcgcagc accccacgcg tcgccagcgc ggcctgttga aagccacaga ggccgaagcg
     4021 gctgagaggc cgcgcctgct cgaccagcac aagttcctgg gagatgccga cgtggaactt
     4081 aacggcatcg agaaggtacg ccgtttcccc tacggactga ggcgttaact gcttgaagcg
     4141 agtgcttgag gcagatgcgt tgatcgtggg ggcggggcca gccggttgcg cggcggcaat
     4201 aaacctcgcc cccttgcgtc aggtcttgtt ggtcgaacgc agggcggagg tcacgccgcg
     4261 cgtcggggaa tcgctcgttc cggcagcgcg gcgtttgttg acggacatgg ggctgtggga
     4321 gagtttcatc gcagagggac acgaaccgtg gtatggaaat cgcgccgtct ggggcgcgcc
     4381 ggagccactc gaagttgact tcatgcgcga ccccgacggg cacggttggc atctcgaccg
     4441 cgcccgtttc gaacaatggc ttcgtgctca cgcaaccaag agaggagccg ggtttatcgc
     4501 ccccgcctcg gtcgatcatc tggcgcacga cggcgagcgt tggcaagtac aactgaacac
     4561 tcctgccagt caatgcacgg cgaacgtgaa gttgatcatc gaggcgggag gacgcgccgc
     4621 acccgtcgcg cgtaagcagg gcgcacgagt tcaagcaagc gacaggttga tctgcgcgtg
     4681 ggtgagcggg cgcgacgcgc gtgagtccaa tcgcgggcta acctatgtcg aggcggttga
     4741 agacggctgg tggtacaccg ctccggttcc gggtcggcaa cgcatccttg ctttttatac
     4801 tgatgccgac ctgccgaacg cgcgagtcat caacaagcga gacggactgt tggaacgcgc
     4861 cgcaagcgca gaggaacttt ccgcgctgtt aactgctgcg ggattcgtgc cggaagctca
     4921 gagccacgtc accgccgcgc atagcgcgat gctcgaccct tgcgcggagg agagttggct
     4981 cgcggtcggg gatgccgcga taagtttcga cccgctctcg tctcaagggc tgttgaacgc
     5041 gctgttcaca ggtttggcgg cggccaacgc ggcggatcgt tatcttgaag gcgatgctca
     5101 ctcattatcc gtctaccggc aggtcgtgcg gggcatcgga gatgcctacc gtcaacatct
     5161 ggcctccttt tatgccaccg agcagcgttg gccggaagcg cagttttggc ggcgtcggcg
     5221 agcctgaatc ggattgaacg agcgacgggt tgtagagatt actttgggca ttaatatagt
     5281 tgacgcacct acaatatcgt tactacctga taaggtaaaa cagacgggta actgttgtta
     5341 tgaaaaaata attattctac aaaagaccat ttttgtggaa ttacatattg gactttacat
     5401 ttgggatttg caggtagagc ttgttgaggt gaaatctgaa agtttagaat gatcttgaat
     5461 aatagttact gcggaatcag ttaagattac aaggggtaca aatatgagca aagtttggtt
     5521 tattacaggt gccggtcgcg gcatgggcat tgacatcgcc aaggctgcgc tggctgctgg
     5581 ctacaaggtt gtcgctactg gccgcaatac cgacatggtg actcaggccg ttggagaatc
     5641 cgccggcttg ttggctgtga agctggatat tacgaaccct gccgacgctg aggcagcagt
     5701 taaggctgct gttgatcgat ttggccgcat agacgtgcta gtcaacaatg ccggtaactt
     5761 ctacgccggg ttcttcgagg agatcacccc cgaggacttc cgggcacaga tcgaaaccaa
     5821 cctgttcggc cccttgaacg tcacccgcgc cgtgctgcct gtcatgcgcc agcaacgctc
     5881 aggcctcgtc gtgacgatct cctcgatagc cgggattgtc gggcaagagt tcgtttccgc
     5941 ttactgcgcg tcgaagttcg gcattgaggg ctggatggag tcgctgaccc ccgaggttgc
     6001 gccgttcggc atccgcacga tgctggtcga gccgggcttc ttccgcaccg aactgctcac
     6061 ggaggagtct acgaaatacc ccgagccctc gatagacgac tacgccgaga agaccgggca
     6121 gaccgtcacc gcctggaagg gcatgaacgg cttgcaaggc ggtgaccccg ccaagctcgc
     6181 caaggcgctc attcagcttg cgagtcagga cgagcctccg ctgcgctggg ccgccggtgc
     6241 tgatgcagtc caggcagtcg aacagaaggc caaagacctg ttagcccagg ccgacgccta
     6301 ccgcgaactg tcctcgtcgc ttgcgcacga caatacgagt gcctgagtcg ctattccaca
     6361 aatgacaacc ttacaagtgc gtcaactata ttaatgcctt aatttgcggc gcgagggttg
     6421 gttcttcggg atcgcttgcg gcggaaggca gagaggcgaa ggaggctgtg ggcgcaattc
     6481 agacgcttac ggctggagtc cgccgtcctc cgaaccggag agccggcgca actccgcgac
     6541 ggcctcttcg acgccgccga gccgttcgcc gtagctaccg gccaggtcag tgagaatgac
     6601 aaacgactct tcgagccgtg cgatgcgtgc atgatcgcgc gcgtgcgatt cggccaactg
     6661 gctcgtcgtc gccgtcaggc gcgcgagtga gtccagtatg aaatccatct gtcgctggcg
     6721 ttcctcgtcc gtcatctcac cacacctctg ccgtcgaaag cgatctaccc gcagcacttt
     6781 tcacggcggc gactttatca aacaactctc gcccatcgca aactttttcc cggcagggac
     6841 acggaagcgc gcggcgtgat tcaacgcctc tctcgtgcgc aacgaatcga aggggcggga
     6901 gttgaacctt aactcccgcc ccttcgattc gtctttagag tgtgacggat gcttcagcta
     6961 tgcgtcgagg caaagctgcg ccgtctattc atagcggagc gcgtccatcg gatcgatctt
     7021 ggtggcgcgg agcgcgggga tgaagctcgc cagcacggct acgccgatga gcaccagcga
     7081 gatcgtgatg aaggtgaaag gatcggtcgc gctgacgttg aacagcaggc tcgacatcac
     7141 gcgggttaac aggaacgacg cgccgaggcc gagcacgata ccggcaatga cgagtttcaa
     7201 accctgcccg accgcgagct tcaacacgtc gcggcgttgc gcgcccagag ccatgcggat
     7261 gccgaactcg cgcgtgcgct gcgccaccga gtagctcatc acgccgtaga tgccgaccgc
     7321 cgccagcacc agcgcgagcg cggcgaagat gccgagcagc agcatgctga aacgttgccg
     7381 cgcgaccgac tccgagacga tctcttccat cgtcctgatc ttggagacgg gctggtcttt
     7441 atccacttcc cagaccttcc tgcgcaccgt cgccgcgagg ctcagcggtt cgacctctgt
     7501 gcggacgacc agatcgttcg gcgcgaagaa tccggcctgc gcgtatgaca ggtacatctg
     7561 cggcttcggg tcggtgacga gttcaaattg ccgtacatcc ttcaccacgc cgaccaccgt
     7621 aatccagtcg tccgggtcgg ttgaagtcgg cgcgcccgaa gtgatgcgcc tgcccgtcgg
     7681 gtcttgctca ggccagtaac ggcgtgccat cgtttcgttg ataacggcca ccgccgggga
     7741 atcaaacttg tcctgctcag agaattcgcg tccctgcaac agggcgatgc tcatcgtctg
     7801 aaagtagtgt gggctgacga ctctcgttgc caccaccgga cgcttgccct gccccggcgc
     7861 gggatcggga atcccctcga tgctgatacc gatggaattg ccctgcatgt acaacggaag
     7921 gttggtggtg acagccgcag acttcacgcc cggcagagcc tctatgcggc tgagcagttc
     7981 gttataaaag gccgtgcgct gcgcgtgctg cggatacttc aactgcggaa gctcgacctg
     8041 catcgtcaac aggttctctg cacgaaaacc cggatcgacg ttgcgcagcc gcaggaagct
     8101 gttgatcagt agtcccgcac caatcagcat gatgagcgaa accgcgactt ccgtgatgac
     8161 cagcaggttg cgtatgcgat tgccgcgact gcccgccgcc gagtctctgc cgccttcttt
     8221 caacgtctcg ttgagattca ggctcgacgc ctgcatggcc ggagctaaac cgaagatcag
     8281 cccggtgagc aacgagacga gcaacgtgaa gcccagcacc cggagatcaa ccgtgatggc
     8341 ctcgacctgc gaaatattct ccgggatgaa ggctttcagc aagctcacgc cccacagaga
     8401 gagcaacagc ccgaccactc cgccgagcac cgccagcaac acgctctccg tcagaaattg
     8461 ccggacgagg cgcaggcggc tcgctccgag tgcgacgcgt atggagatct ctttctgtcg
     8521 caccgccgcg cgcgcgagca gcaggttggc gacgttcgcg caagcgatca acaggacgaa
     8581 cccgaccgcg ccgagcagca cgagcaacgc cggtttgatg tcgcccacga cctgttcgtg
     8641 cagaggcgtg accgccacgc cgaggtctgt gttctgttcg gggtactgtt gttgcagctg
     8701 cgccgcgatg gtgctcatct cggcctgcgc ctgttcgagc gtgacgccgg gcttgatgcg
     8761 cgcgacaact tccagataat gcctgccgcg attggcggcc tcctgcggcg tgaaggcgat
     8821 gggaacccac agttcatctt cgcggcgggg aaactggaag tgcggcggca tgacgccgac
     8881 caccgtgtag ctctcgccgt tcagggtgag cgactgatcg attattttcg cgtccccacc
     8941 gaagcgtctc tgccacaggc cgtagctgat gacgacgacg cgattccgcc ccggctggtc
     9001 ttcttcaggc agaaaggcgc gccccacctg cggctcgacg cccagcagcg aaaagaggtt
     9061 ggccgagacg cggcggcctt cgatcttttc cggctcgcca gtccccgtca ggttgaagtt
     9121 ctgttcggcg atggccgcca tcccttcaaa gaccccgttt cgatcacgcc agtctacgta
     9181 gttggcagcg gcgggcgtgt cgcgcgggta gccgcccttg gtgtcgtcct cccacaccat
     9241 cacgagccgc tccgggtctt tgtaaggcag agggcgcagc aagaccgtgt tgaccacact
     9301 gaagatcgcg ctgttcgcgc cgatccccaa agccagcgca agcaccgcga ccaccgtgaa
     9361 gccggggttc ttcaacatca ttcgcaggcc gtaacgcagg tcctgccaga ggtcagtcat
     9421 gatattcgtt ctcccacgcg cgccgagcac caccggctcc tgctgcaccg gacgttccac
     9481 ccgtcgcagt tcatgagcca gaagattgct ctcgtttaat tcgagcaacg ccgcgcggta
     9541 ggcttcctca cgcgtcgcgc cgccctgtaa caactgctcg aaacgatcgt cgagatgctg
     9601 cgccaactcc tcgacgatct ccgcctcgcg cgtcggcgcg agtttcaact ccgacaacag
     9661 ccgtctgatc tcgtctttcc aatcaggcat gttcgattcc cacgatgcga ttgatcgctt
     9721 cgacaaactc tcgccagctg accagttgcg ccgccagaac ctttttgccc gcaggggtca
     9781 ggcggtaata acgacgccgc cgctgcccgg ccttctccac ccagcggcct tgaatccagc
     9841 cgcgattctc caggcggtag agcagcgggt agagcgaggc gacgttgaaa cgcaacagcc
     9901 cgcgcgaacg ctgctcgatc aaattgccga tgtcgtagcc gtgccggggc tgatcttcca
     9961 ccaacgacag aatcaacaat tccgcgctgc ccttcttcaa ctcgcggtcg agcatgtttg
    10021 aaccgatata tgacatagcc atatattatt aagaaacata ggagccgtca acaggaatct
    10081 tcgcacataa atttggatcg tcgaggctta aaaatttgga tggtcggggg ctttatttaa
    10141 ctttgaggac gaggagcgtg aggtcgtcgt gctggcgtgc tccggcgcac cattcgcgga
    10201 ggcgagaggc gacgcggtcg cggatttcgt cagccgtcgc gtgtgtcgtc gctgcgagtt
    10261 cgaccagtaa tctctcttcg ccgtactctt cgcccgcgag attgagcgcc tccgtaacac
    10321 cgtctgtgta ggcgaagagc aggtcgccgc tctgcatgtc gagcgtctct tcttcgtagc
    10381 cgaagtgctc gaagacgccg atcaccgcgc cgcccgtcgc cagtttcaga tagtcgttca
    10441 ccggaggctc gcacgtctcc gcgccctccc gcctgcggat caggaaaggc gggttgtgcc
    10501 cggcattaac gtaagtcaac ttgccttcgc gcgcgtcgaa ctgcgcgtag aagaaggtca
    10561 cgtaactcgc aatgccggtt gagcgacaga gcaggcggtt catggccgag acgagattag
    10621 ccagcgaacc ggccacggac acgctggcgc agttggctaa cgcctggctg cgcagagacg
    10681 cctgcacggt tgacatgagc agcgcggcag aaatcccttt ccccgctaca tccgcgacgg
    10741 tgattccggt ctgcccgttg tcgagtgtga gaaagtcgta atagtcgccg ccgacgccgc
    10801 gcgcgggctg gcacagaccc gacaagtcca gcgtgtcgga ggtcggcggg tgcgcgggaa
    10861 agagtctctg ctgcacctcg gcggccagca tgagttcgcg ccggagacgc tcctcctcca
    10921 ccatccgctc gatgagtctc gaattctcga tgacgagcgc caactgcccc gtgatggaca
    10981 tcaacagctg ctcgtccttc ggcgaaaact cctgcgcaga ctgtttccga ccgattgaga
    11041 ggatgccgac cagctgatcc ttcatcttga tttgcagcat caggctcgac cgcagactcc
    11101 gcaacgtcgc gcactctgcg gcgcgttccg cttgcagcgt gggcggggcg ttcgccagtg
    11161 cgcgcaccca cgtctccaaa tcctgcggct cgatgtgcag cgggatcgcc agattgcgca
    11221 ggcggttgac gacgaacgcg tcgctccgaa gcgcgggcgt cgcggctcgc tcttcgtgtg
    11281 cgggcgactg cgcttccgcc ccggcgtgcg gcttcggcgc gtgctgttcc gccgacatac
    11341 gacagacgaa ctctccgctc gcgccctcgc gcacgaagag cgagacgttt tcggagcgca
    11401 acgcgtcgcg aatcatctcg cagatgtact tgtagagttg gtctgtgttg gtcgcggcgc
    11461 gtgcggcttc gctaaggtcc aggaggatgc gtcggtcgtc gtaagtcgga ggcgagaagc
    11521 ggcgttcgag cagcggcagg aggctccggc ggatgaagag atgcaggagc gcggcgg
//
LOCUS       DASWNS010000520         3924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_356969, whole genome shotgun sequence.
ACCESSION   DASWNS010000520 DASWNS010000000
VERSION     DASWNS010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356969"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VGB76_17920"
     CDS             <1..228
                     /locus_tag="VGB76_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100829.1"
                     /translation="QMKFVNYRELTAGRMRWHGSNFKSAMDCFCSIVAADWQRNSSPL
                     SRRVNFRLRFERMPGAPRQIHLDGTKFNDKL"
     gene            314..2497
                     /locus_tag="VGB76_17925"
     CDS             314..2497
                     /locus_tag="VGB76_17925"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0100830.1"
                     /translation="MSKYHSTFARRLLVTAALCLSASALLWNQNVSRAQATRPILISE
                     AASTRAVAVESVTRLHEPFSRDARHAFSPDRRTRIQLFVMNLERGVEASALTAEAEDG
                     AHRVYPLTVESVNLVPGQEWMHSLTLRLNDEMGDVGDVLVRVRYKNAASNRVRVGIGR
                     IGGGLPDDAGATPTAAPPSAAPTPNTNPVTAGTLTTSDVQNVIAQAVSAAVALNRAVT
                     VAVTDREGNVLGVFRMTGAPDQTQFRGNSGFNQQPNSPAGLVASGLEGTNLIPALKPV
                     PRAAITKAGTAAFFSTQGNAFTPRTAGFIIQEHIPPGVDFRPGGPLYGVQFSQLPCSD
                     IKTPGLPFGLSADPGGVPLYKNGVAAGGVGIEGDGAYTVDRIPADFDKPFEEIIAVAA
                     GRGYEPPPLIRGDNILVDGVRLPYVNVTDADAPRPATLPFASLPGTIDPDFPLRSAQP
                     SAFVPLTLGTVNGAIDTRFFPFTGSMSGSPNALNASDVTTIISQAAIQADITRAAIRQ
                     PLGSPARVSISVVDSEGRVLGIFRTTDAPVFGFDVSAQKARTAALFSRINAGALLRGA
                     GVGSYVDRAVADGIRLDGSIAFSDRAIGFLHRPFYPDGINGEPPGPFSTGIGQWSIFN
                     VGLQLDLVKTNLEATLAGTPNLPCTSIPNVPNGLQIFAGAVPLYKNGELVGAIGISGD
                     GIDQDDLIAAAGSRGYVAPAEIRADQLIIRGARLPYVKFPRSPNL"
     gene            complement(2591..3352)
                     /locus_tag="VGB76_17930"
     CDS             complement(2591..3352)
                     /locus_tag="VGB76_17930"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100831.1"
                     /translation="MNKNILLCVVGLVLGFGVGFVIANKLTGGAIPAAAPASAPRAAS
                     DSAPPLNPDQAGAPLPPGHPDVNSAAAAAGNSNPNGVAATNAEAQAAMEAADRKPQDF
                     DLQMNAATTFYKLKAFDKATLYLERAIKLNPKDTDALIAMGNTKYDAGDFIAAASFYE
                     RALAVEPQNTDAQTDLGNTYFQRQPPDFKRAIEEYRKTVKINPRHEKAWQNIAAAALQ
                     MGDKSTAREAIEQLSSVNPANQYLERLRANLESTP"
     gene            3702..>3924
                     /locus_tag="VGB76_17935"
     CDS             3702..>3924
                     /locus_tag="VGB76_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100832.1"
                     /translation="MSAIEETKNEIRVQRVAPAKATPSVITRLLNLLSSVRFGVIMLI
                     LLAVASMVGMLVMQSNVDGFDKYFAGLTPS"
BASE COUNT          714 a         1276 c         1161 g          773 t
ORIGIN      
        1 caaatgaaat tcgtgaatta cagagaacta accgctggtc gtatgcgctg gcacggaagc
       61 aatttcaaaa gcgcgatgga ctgtttttgt agcatagtcg ccgccgactg gcaacgaaat
      121 tcttccccgt tgtcgcgccg cgttaacttc cgtctgcggt tcgagcgcat gcccggcgcg
      181 ccgcgacaaa tacatcttga cggaacaaag tttaatgata aattgtagcc gctccggtta
      241 atatcccaac gccgcaagcg cgtcgccggt ttctgcactg tatcgccaga acgcaattca
      301 cacattgctt cctatgtcta aatatcattc aacctttgcc cgtcgcctgc tcgtcacggc
      361 tgcgctctgt ctctcggcca gcgcgctcct ctggaatcag aacgtctcac gggcgcaggc
      421 gacgcgcccc attctcatca gcgaagccgc ttcgacgcgc gccgtcgccg tcgaatccgt
      481 cacgcgcctg cacgaacctt tctcccgcga cgcgcgccac gcgttcagcc ccgaccggcg
      541 cacgcgcatt caactcttcg tcatgaatct cgaacgcggc gtcgaagcct ctgccctcac
      601 ggcggaggcg gaagacggcg cgcatcgcgt ctatcctttg acggtggaga gcgtcaatct
      661 cgtgcccggt caggagtgga tgcactctct gacgcttcgc ttgaatgatg agatgggcga
      721 tgtcggcgac gtgctggtgc gcgtgcgcta taagaacgcg gcgagcaatc gcgtccgcgt
      781 cggcatcggg cgcatcggcg gcggcttgcc ggacgacgcg ggcgcaactc cgaccgccgc
      841 cccgccctcc gccgcgccca cgccgaacac caatcccgtc accgccggaa cgctcacgac
      901 gagcgacgtg cagaacgtca tcgcgcaggc cgtctccgct gcggtcgcac tgaatcgcgc
      961 cgtgacggtc gccgtcacgg acagggaagg caacgtgctc ggcgtcttca ggatgacggg
     1021 cgcgcccgac cagacgcagt ttcgcggcaa ctccggtttc aaccagcaac cgaactcgcc
     1081 cgcgggcttg gtcgcatccg gcctcgaagg cacaaacctg atccccgctt tgaaacccgt
     1141 gccgcgcgcc gcgatcacga aggcgggcac ggctgccttt ttcagcacgc agggcaacgc
     1201 cttcacgccg cgcaccgccg ggttcatcat tcaggaacac attccgcccg gcgtggattt
     1261 tcgtcccggt ggtcctctct acggcgtgca gttctcccaa ctcccctgct ccgacatcaa
     1321 gacgccgggc ttgcccttcg gtctttcggc agaccccggc ggcgtgccgc tctacaagaa
     1381 cggcgtggcc gcaggcgggg tcggcatcga aggcgacggc gcgtacacgg ttgaccgcat
     1441 ccccgctgat ttcgataaac cgttcgaaga gatcatcgcc gtcgcggcgg gacgcggcta
     1501 tgagcctccg cccctgattc gcggcgacaa catcctcgta gacggcgtgc gcctgcccta
     1561 cgtcaacgtg acggacgcag acgcgccgcg ccctgcgacg cttccgttcg cgagtctgcc
     1621 cggcacgatt gatccggatt tcccgctccg ttcggcgcag ccttccgcct tcgtgccgct
     1681 cacgctcggc acagtcaacg gcgcgattga tacgcgcttc ttcccgttca cgggcagcat
     1741 gtccggttcg ccgaacgctc tgaacgcgtc ggacgtaacg accatcattt cgcaagcggc
     1801 gatacaggcc gacatcacgc gcgcagcgat ccggcaaccg ctcggcagcc ccgcgcgcgt
     1861 ctccatcagc gtcgtcgatt cggaagggcg cgtactcggc atcttccgca cgacggacgc
     1921 gcccgtcttc ggcttcgacg tgtccgcgca gaaagcgcgc accgccgcgc tcttctcacg
     1981 catcaacgcc ggagccttgc ttcgcggcgc aggcgtcggc agctacgttg accgcgccgt
     2041 cgccgacggc atacgcctcg acggctcaat cgccttcagc gaccgcgcca tagggtttct
     2101 ccatcgcccg ttctacccgg acggcatcaa cggcgagccg ccgggtcctt tcagcaccgg
     2161 catcgggcaa tggagcatct tcaacgtcgg actccaactc gatctcgtca aaacgaattt
     2221 ggaggcgacg ctggccggaa ctccgaacct gccctgcact tcgattccca acgttccgaa
     2281 cggcctacag attttcgcgg gcgcagtgcc gctctacaaa aacggcgaac tcgtaggggc
     2341 tatcggcatc agcggcgacg gcattgatca agacgatctc atcgccgccg ccggaagtcg
     2401 cggctacgtt gcgcccgcag agatacgcgc cgaccaattg atcatacgcg gcgcgcgtct
     2461 cccttacgtt aaattcccgc gcagccccaa tctctgagcc gcccgcgacg ccaaaagcga
     2521 aacgcccgca gcgacacata gcgggcgttt cgtttcctgt ctcagacgtt tgaacttcga
     2581 cgtgcgaaac ttaaggtgtc gattcgagat tcgcgcgcag acgttcgaga tactggttgg
     2641 ccgggttgac cgaggagagt tgctcgatgg cttcgcgcgc ggttgatttg tcgcccattt
     2701 ggagggctgc ggcagcgatg ttctgccatg ccttctcgtg gcgcgggttg atctttacag
     2761 tcttgcgata ctcttcgatg gcgcgtttga agtcgggcgg ttgacgctgg aagtaagtat
     2821 tgccgaggtc ggtctgcgcg tctgtgttct gcggttcgac ggcgagcgcg cgttcgtaaa
     2881 agcttgcggc ggcgataaag tcgcccgcgt cgtatttcgt gttgcccatc gcgatgaggg
     2941 cgtccgtgtc cttcgggttc aacttgatgg cgcgttcgag gtagagcgtg gctttgtcga
     3001 acgctttgag tttgtaaaag gtggtggcgg cgttcatttg caggtcgaaa tcctgcggct
     3061 tgcggtcggc ggcctccatc gcggcctgcg cctcggcgtt cgtcgcggcc acgccgttcg
     3121 ggttcgagtt accggctgcg gctgccgcgc tgttcacgtc cgggtggccg ggcgggagtg
     3181 gcgcgcccgc ttgatccgga ttgagcggtg gagccgaatc ggacgcggcg cgcggcgcgc
     3241 tcgccggagc cgccgccgga atcgcgccgc cggtgagttt attggcgatg acaaaaccca
     3301 cgccgaagcc gagcacgagg ccgacgacac acaggagaat gttcttgttc atagacgttt
     3361 gaaacgtagc gacctttcgg aaaagttagc ttgcggcatc gtgaaaacag cctgttgcct
     3421 caggctgaga gaaacagaga acgacgactc gacgcctctt cctctccggg aaattcgcat
     3481 gatacacgat gcgtccgcgc agctaaaacg gagcgcgcgc ggcgcatgcg gagcgtacac
     3541 gacgttactt cgggctgcgg aaaaacgttt gtgcgataag ttgatgatga agcagtgatc
     3601 ggctatgatg accaccacac gcagtaacgc gcgattcgac ctcgcttgcg ttaaatttca
     3661 aagatgtttc ctcaagtgta gttgctgaac ttcataaata aatgtctgcc atagaagaaa
     3721 ccaaaaacga gatacgtgta cagcgtgtag cccccgcgaa agcaaccccc tcggttatca
     3781 cgcgcctgct taacctgctc agttccgtgc gcttcggcgt catcatgctg atcctgctcg
     3841 cggtcgccag catggtcggc atgctcgtca tgcagtcgaa cgttgacggc ttcgacaagt
     3901 acttcgccgg tttgaccccg tcgc
//
LOCUS       DASWNS010000521         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_97576, whole genome shotgun sequence.
ACCESSION   DASWNS010000521 DASWNS010000000
VERSION     DASWNS010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97576"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VGB76_17940"
     CDS             <1..528
                     /locus_tag="VGB76_17940"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY0100833.1"
                     /translation="GADVILIPEIPFSLKVVAEKIEERERCDSQFSVVVVAEGAREAG
                     GMEIYQDAGDREHAPRLGGIGHHVARQLERLTGKETRCVVLGHLQRGGTPNAFDRMLA
                     TNYGSCAVRALMRGERGKMVALHAADIVTIPLSKAIANIKTVPLESQLIRTARDTGVS
                     FGAIDEAEHHGKAGC"
     gene            667..1167
                     /gene="greA"
                     /locus_tag="VGB76_17945"
     CDS             667..1167
                     /gene="greA"
                     /locus_tag="VGB76_17945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY0100834.1"
                     /translation="MTQNVKDRLQAELDELDTELRVHLPKQIKIAKEWGDLRENAEYH
                     AALARQQFVQARIGQLRQRLREISSIDVTKVPHDKAAYGSTLLLYDAERDEEVTYRLV
                     TPEESDPQAGLISTTSPVGKSLMGREEGDEVTVRTPNGARNFEVRRLTTLHDELKAKE
                     SDAASD"
     gene            1506..2246
                     /locus_tag="VGB76_17950"
     CDS             1506..2246
                     /locus_tag="VGB76_17950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0100835.1"
                     /translation="MFGASIGRGAQRIISAMVRWLAYGHINPNLLTVIGVAINVLSGL
                     LFGFGSFFWAGIVLIIANLFDMLDGQVARLSGRVTRFGGFLDSSLDRLSDMVVFVGLM
                     VFYARDTPFHSTLNVFLAGTAMMGSVMVSYASARAESLIPKCDVGFLRRPERVVLLII
                     GGLSTHPGSANYFANRMPAVLWILAIGSYWTFAHRMYHTWREVWRADEAIAAAQAAKD
                     ASKTGRAGTGGSDAKENSSTSKIAPAPH"
BASE COUNT          494 a          765 c          728 g          491 t
ORIGIN      
        1 ggcgcggacg tgatcctcat ccctgaaatt cccttttcgc tcaaagtggt ggccgagaag
       61 atcgaggagc gcgagcgttg cgattcgcag ttcagcgtcg tcgttgtggc cgaaggcgcg
      121 cgcgaggcgg gcggcatgga aatctatcag gacgcgggcg accgcgagca cgcgccacgc
      181 ctcggcggca tcggccatca cgtcgcgcgt caactcgaac gactcacagg caaagagacg
      241 cgctgcgtcg tactcggtca tctccagcgc ggcggcacgc cgaacgcttt cgaccgcatg
      301 ctcgccacca actacggctc gtgcgccgtg cgcgcgttga tgcgcggcga gcgcggcaag
      361 atggtggcct tgcacgcggc ggacatcgtt accatcccgc tctcgaaggc catcgccaac
      421 atcaagaccg tcccgctcga aagccagttg atccgcacgg cgcgcgacac gggcgtctcc
      481 ttcggcgcga ttgacgaagc ggaacatcac ggcaaagccg gttgctgata gagacaaggg
      541 caaggctaga ggtaagagca gcaaaagacg ggcaacgcta gcgacccggt tgcgatccct
      601 ccctttccct ttaccccttc cccttacttt gtttatattc tgaaaaacgg agtggactaa
      661 aagacagtga cacagaacgt aaaagaccgt ctgcaagcgg aactcgacga actcgacacg
      721 gagttgcgcg ttcatcttcc gaaacagatc aagatcgcga aggaatgggg cgacctgcgc
      781 gagaacgccg aatatcacgc cgcgctcgcg cgccagcagt tcgtccaggc gcgcatcggc
      841 caactacgcc agcgtctacg cgaaatctca tccatcgacg tgaccaaagt gccgcacgac
      901 aaagccgcct acggctcgac gctgctcctc tacgacgccg aacgcgatga ggaagtcacc
      961 tatcgcctcg tcacgccgga agagagcgac ccgcaggccg ggcttatctc caccacttcg
     1021 cccgtcggca aaagtttgat gggacgcgaa gagggcgacg aagtgactgt acgcaccccg
     1081 aacggcgcgc gcaacttcga ggttcgacgc ctcaccaccc tgcacgacga gttgaaggcc
     1141 aaagaatctg acgcggcgag cgactaaaac ttgcgcgcga tgctcgactc ccgccccgtt
     1201 cgacttgtct cgtcatctca gaaatagaat cttcgacgta gttttgcggc gtcccggtga
     1261 gcgttaagct cgccgtctgt gctttgaagt tgtgagagat ggagcatgga taaaagcgtc
     1321 gcaattcagt cgctcttcac gaccaccgca cttatcgtta cctatctcgc tgctattcag
     1381 ggcgcggggc gagacacttt tcttgacaag cacttgacca catccctatc atgcactgtt
     1441 tatttcggag acgcgggcgg taaatcgtct ccgggcgggg ttttagaaga aaggctgtaa
     1501 gagagttgtt cggggcgagc atcgggcgcg gggcgcagcg aatcatcagc gcgatggtgc
     1561 gctggctggc ctatggtcac atcaacccga acctcctgac cgtgatcggc gtcgccatca
     1621 acgtgctctc cggtctgctc ttcggcttcg gaagtttttt ctgggcgggc atcgtgctca
     1681 tcatcgccaa cctgtttgac atgctcgacg ggcaggtagc acgcctgtcg gggcgcgtga
     1741 cgcgcttcgg cggcttcctc gattcctcgc tcgaccgcct ctccgatatg gtggtcttcg
     1801 tcgggttgat ggtcttctat gcgcgcgaca cgccatttca ttcgacgctc aacgtgtttc
     1861 tcgccgggac ggccatgatg ggatcggtga tggtttccta cgctagtgcg cgcgccgaga
     1921 gcctcattcc gaagtgcgac gtagggtttc tgcgccgacc ggaacgcgtg gtactgttga
     1981 tcattggcgg cctctcgacg catcccggct ccgccaacta cttcgccaac cggatgcccg
     2041 ccgtgctctg gattctcgcc atcggttcct actggacgtt tgcgcatcgc atgtatcaca
     2101 cctggcgcga agtttggcgc gctgacgagg caattgcagc agcgcaggcg gcgaaagatg
     2161 catctaagac gggtcgcgcc ggaacgggcg gcagcgatgc caaagaaaat tcgtccacat
     2221 cgaagatcgc ccccgcgccg cactgaaggg ctggccggaa cagtttcggt cgtttgcgcg
     2281 ttattcacac aggttgtcgg ttgagttcgt gcgtcatctt aaaatttgag gtctcccaca
     2341 ttgaatattt gtccatgctg cggttcgaag acgaacggcg atctgctgac ggaaccctgt
     2401 caagcctgcg gcgcacacgc cgtcggcccc ccgctcgcgc gtcctgaacg cgaactcccc
     2461 tcctacttga gcgcgttt
//
LOCUS       DASWNS010000522        12941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_202048, whole genome shotgun sequence.
ACCESSION   DASWNS010000522 DASWNS010000000
VERSION     DASWNS010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12941
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202048"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            772..2814
                     /locus_tag="VGB76_17955"
     CDS             772..2814
                     /locus_tag="VGB76_17955"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0100836.1"
                     /translation="MKRVLALLFTVALCAVVFTPHFTQGQGRERSKLRKARQAIPNQY
                     IVVLKNTLPGSNVASVANELARQHAGNLRFVYEHALRGFSVQLPEAAAIALSQNPLVE
                     YVEENSEGSVVDTQFNPPWGLDRIDQRDLPLNNTYTYNANGAGVNAYIIDTGILASHV
                     DFGGRASLVADYVNEGCGDCYGHGTHVAGTVGGSTYGVAKAVTIRAVKVCNGGGGCAL
                     DATIAGVNYVTQQKQNNPGVPAVANMSLRFFPTTSLDNAVRTSINAGVTYAIAAGNDS
                     DFAANYSPARVTEALTVGATDSSDNKAWFSNYGSVVDIFAPGVGVTSAWIGSNTATLA
                     LDGTSMASPHVAGVAALYLQSNPGASPATVNQAIINNATPNRIPDPGLGSPNRLLYSL
                     FTPLANRDNRADFDGDQRTDISIFHRGTGQWLSRNSSNGVDVSYNPPFGQSGDIPTPG
                     DYNGDGKTDRAIFRPGNGTWYIATDTSGAFYTVQFGQTADIPVARDYDGDGRTDQAVF
                     RPGIGTWYILNSSNGTWTVVQFGQSGDRVVPGYYDTDNKADIAVFRPSNATWYIRHAD
                     GSFHGVQFGVVSDWPVQADYDGDGLTDIAIFRPGGGVWWILRSSNNTAYSVPWGTSGD
                     RPVPGDYDGDAKADIAVQRPDSNGTWYILKSTTGTYDAVNFFSEYPVPGGYLTPLY"
     gene            complement(2962..5301)
                     /locus_tag="VGB76_17960"
     CDS             complement(2962..5301)
                     /locus_tag="VGB76_17960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0100837.1"
                     /translation="MTKETSLESHELFRLIVENIRDYAVFVMNLEGQAISWNPGVESL
                     LGYGEGEWVGEFASIIFTPEDHEQGAHLLELETALRDGRAEDKRWHVRRDGSRFWAEG
                     VLMLLRDGQDSPRGFAKILRDNTEAKLTEERLRESEERFQRLVELSPDGIAVHAGGVV
                     LFMNTAGARLLHAESPEQIVGRSIASLIHPDYHGLVRERIERVSRGETPPPSEIKFIR
                     LDGTEIYGELASAPLLYQGRPAVQAVVRDLTRRKQAEEALRESEKRFRLIFNQQFQFM
                     AILSPEGFLLDINELPLRAAGVTREGVLGRLFWETPWWDGLTEMQAGWPARLASAARS
                     DTPVFSVDSYQAADGTRVADASITAVKAVDGSVEFFIVQASDITERKRVEEERERLLR
                     EAQEANRLKDEFLATVSHELRTPLTAILGWAQLLRGGQLEGEAAVRALDTIERNARSQ
                     AQLINDLLDVSRIVTGNLRLDVVPVSPHSFIDPAVEAVRPAAEAKGVRLQKVIDTGVL
                     TVMGDPSRLQQVVWNLLTNAVKFTPRDGRVQVRLERVNSQIEIAVSDTGAGIEPEFLP
                     HVFERFRQADQRTTRQHGGLGLGLAIVRHLMELHGGTVRANSAGEGTGATFTVTLPIA
                     AVHRREETEERTPEAARDMSPAHDCPERLDGLKALVVDDEPDARELLSVGLGQCGAQV
                     TMASSARDALAALAAAKFDVLISDIGMPGEDGYELIRRVRELPPGAGGRTPAVALTAY
                     ARTEDRLRAMRAGFEMHVSKPVELAELIAIVANLVGRAH"
     gene            5641..6489
                     /locus_tag="VGB76_17965"
     CDS             5641..6489
                     /locus_tag="VGB76_17965"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEY0100838.1"
                     /translation="MKRQAHERVEATDVPSSGRRARLHEIIFEADTRAGRVFDFALIW
                     LILLSVATVILESVRYVRVQYGELLYALEWLFTLLFTIEYILRLLSVRRPLRYATSFF
                     GVVDLLAIIPTYLSIFVPGSHYLLVIRILRLLRIFRLLKLAEYITEAATLRRALRASQ
                     RKISVFLSVVVLLVIIIGALMYVVEGEEHGFTSIPVSIYWAVVTLTTVGYGDLSPRTP
                     FGQMIASIVMIIGYGIIAVPTGIVSVELAQAAREQNVSRQSCPACGAEGHDPDAFCCK
                     YCGAKL"
     gene            complement(6573..7343)
                     /locus_tag="VGB76_17970"
     CDS             complement(6573..7343)
                     /locus_tag="VGB76_17970"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY0100839.1"
                     /translation="MEWMADPQTWIAFLTLTVLELVLGIDNIVFISILAGKLPPEQQA
                     RARYIGLGLALMMRVILLFSLTWVIGLTAPLFAVFGQEISGRDLVLLLGGLFLLAKST
                     HEIHGSLEGEEGHKSAKIYPSFVGVLIQIALLDIVFSLDSVITAVGMVDNLWIMIAAV
                     VVSIIFMMAFAGSIGAFVQKHPTIKMLALSFLILIGVTLIAEGLDQHIPKGYIYFAMA
                     FSVLVEILNLRLRKAKTAPVQLHNAYDSTDEAKALQME"
     gene            8099..9001
                     /locus_tag="VGB76_17975"
     CDS             8099..9001
                     /locus_tag="VGB76_17975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0100840.1"
                     /translation="MSLIIPTHSRPQLLVRAVESAKLAGRDVELLVVDDASTDGTAEV
                     CRALEGIRYIRLEHNLGVAGARNAGILASSAEYIAFLDDDDLRLPASLDLQLAALARQ
                     PEAGFVCGSMLLADQEGRLTGEKSAPVHPGGDLFWELMELAFPVMPMSVLFRRECFFR
                     AGLFRNRLAGIDDWDIFVRIAELYPVVVISEAVGIYRQPTPSSGQGSSQLARHLTRAV
                     SHQRELLRLPRAMAASAERRRQARRAAINRVANTLLRNAAEGLPTGSYGFVCANILSA
                     LRLDPLYVARPGVYKKLFRRLLAT"
     gene            9086..10054
                     /locus_tag="VGB76_17980"
     CDS             9086..10054
                     /locus_tag="VGB76_17980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEY0100841.1"
                     /translation="MPEISLIITTHNRPQMLARAVESAQKATRSEVEVVVVDDASTDE
                     TARLCRSIPHITYVRAERNQRVAGARNLGILASRGEFLSFLDDDDLRLPGSLDPQLAA
                     LASDPRAGFIYGQAILSGREGAAEEDRYPACCPQGDIFFRLLVQNFIPCGSVVFRRAC
                     LSRVGLLDDALAGIDDWDLWLRIAALYPVIAVEQPVVRWRKSTPVSRQGSSRALEMVS
                     LSTGQFHKSWLALSRVAETPASQRRECSRRFSENMAGHLLWETWRAFATGEVTAACRN
                     MLGAMRLHPVAMIRVAARRTSLRFFLTHAPGEWRDLKARTGQLPNS"
     gene            10069..11364
                     /locus_tag="VGB76_17985"
     CDS             10069..11364
                     /locus_tag="VGB76_17985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0100842.1"
                     /translation="MKIILGKGQFMGPVSGADETLVAYATQLRLAGHSVSVLLMYPHM
                     AGDQYFQRLSKADVPVFSIAPASVRAYLDAGRNLLRGLLQVFPMPRRLLRSRAQRLAS
                     SVADQYFDQCCDYLRRLNADLVHVITPDPSAMIMIRAASAVGLPVFYQELGIPYHPPE
                     FESYYKEFTSVLPLCSEVAALSPQLALHCREKLPHANSLSVLPIMASAPFHQVTTSAK
                     APPTQPTAGVTFGFAARFEYLKAPHVLLEAFARVYRERDDVRLRLVGVGSLRRKIMAR
                     AAALGVAHACDFPPVYTQLEEKGAFMRSLNVFVLPSLTEGTPNSIMEAMAHGLPVIST
                     TVGGIPDVVTAETGILVPPEDTEALAGAMLRLAGDPALRARMSEAAKFRYEELFSPDS
                     VLPVVMNRYRAVISGRNNHQPDDKPESNGLSHPWANTYF"
     gene            11429..>12941
                     /locus_tag="VGB76_17990"
     CDS             11429..>12941
                     /locus_tag="VGB76_17990"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0100843.1"
                     /translation="MPHLLGEIKYEHLHRYALAQEFVKGKEVLDIACGEGYGAALMSA
                     AARSVVGVDIDAAVIEHARRKYAAHPNLVFRTGSCDSIPLPDRSVEVVTSFETIEHHD
                     QHQEMMREVRRVLRPGGVLIISSPNRPVYSELYGSSNQFHVKELDYQEFTDLLKEHFH
                     HIAVYGQKLEAGSFVFSVDDTPGRDFTAYSGKGEQLRGALPPLSAPSYFIAICSDAEL
                     GIAPGLESIYLDHGDSLQEHALEELRRLRADHQQAVELYLRHALELEEIKSHISFKLI
                     SRLLWPMTGKLLPQSIRSRTGKNLSRMKRTPAALALARKAEKLRFEINENISQPFVIG
                     KGNLYYLSGWCYHPRHSLRRLYVLVDDEPHEVFNHSLAETEACRDHSSDHELSGNCLT
                     SRFWTALPFSQLDAPREVKLSLRAILSDESVSEAKLGTLRLLPSPPPESRAEWNESAD
                     VASSWPVAGDGARVVICMATHQPPLDLFALQIKSLIAQSHRNWLCLISDDCSEP"
BASE COUNT         2601 a         4023 c         3685 g         2632 t
ORIGIN      
        1 ctacttcgcg cgcgcgacga cgcccggcct gttcgtcgtg ccccctgcga aagcggaaga
       61 gatgtactcg cccgaaacct tcgggcgcgg ggcgagtgat cgcgtgcgcg tggaatagcg
      121 tgccctgtag caggagatga attcacgtct ctagtcgcgc gactcttcca tctctgacgc
      181 tttcagagct tgagacattc ctgtctactt tttcgctccg accgacacgt cgcagcggag
      241 actacgcgca accgggcacg tcgcctaaca acggcgtgcc cccgaaaaga actccgttaa
      301 atcagagcac taaagccccc tcctgtccgg caggattttt agcaactccc gcacgcttcg
      361 gccaggctcc gagcacgtca gacgactcga caaaagacag agactttggc gaaaagtgcg
      421 acacgaagcc gacgttagaa agcctgcttg atggcctgag cgggtaacgc taaatacacg
      481 ttgacacctc ttcacagggt tgtgtaaggt gtgcttgctc ccaagctcaa ttcgtcggcg
      541 agatacccct ctcgttggcc tcatataaac aacagataag gaatcccgtg cagttacctc
      601 gcgctgtacc actctctctg tcggaaagct gctgcgcagt tctctccgtc agaagtgtat
      661 cgaccagaag cgtatcggca ctgcgccggg aaatttcttg tcagtcgcct cacccggatt
      721 gcgtcatggc gaccccgtta actcgaagcc aaactcacga aaggaaccaa catgaagaga
      781 gtcttagcat tgctctttac agtcgcgctg tgcgctgttg tcttcactcc tcactttact
      841 caaggacagg gtagagagcg gagtaagcta cgcaaagcca ggcaagcgat tcccaaccag
      901 tacatcgttg tccttaaaaa cacccttccc ggatcaaacg tcgcctctgt cgccaatgaa
      961 ctcgcccgcc agcatgccgg aaatctccgc ttcgtctatg aacatgcgct cagaggcttt
     1021 tcggttcagt tgccggaagc tgccgctatt gctctgagcc agaatccgct ggtggagtac
     1081 gtggaagaga atagcgaggg ttcagttgtt gacacacaat tcaacccgcc ctgggggctt
     1141 gatcgcatcg atcagagaga cttgccgctc aacaatactt acacctataa cgccaacggc
     1201 gcgggcgtta atgcctacat catcgacacg ggcattctcg ccagccacgt ggatttcggc
     1261 gggcgcgcct ctctcgttgc ggattacgtc aacgagggtt gcggcgactg ctatggtcac
     1321 ggcacgcatg tcgcgggcac tgtcggcggg agcacgtatg gcgtcgccaa ggctgtgacg
     1381 attcgcgccg tcaaagtttg taacggcgga ggcggttgcg ccctggacgc gaccatcgca
     1441 ggcgtcaact atgtgacgca gcaaaagcag aacaatccgg gcgtcccggc agtagccaac
     1501 atgagcctgc gcttcttccc cacaacttct ctcgataatg ccgtgcgaac ttccatcaat
     1561 gccggagtga cctacgccat cgccgcggga aatgactcag acttcgccgc caactactcc
     1621 ccggcgcgcg tcaccgaagc gttgacggtt ggcgcaacgg acagttcaga caacaaagct
     1681 tggttttcta actatggttc ggttgtggat atatttgcgc cgggcgtggg tgtcacctcg
     1741 gcatggatcg gcagcaacac cgcgaccctc gctctcgacg gaacctctat ggcgtctccg
     1801 catgtggcgg gcgtcgccgc gttgtatctg caaagcaatc cgggagcttc acccgccacg
     1861 gtcaatcagg cgatcatcaa caacgcgacg cccaaccgga tacccgaccc cggcctcggt
     1921 tcgcccaacc gtttgctcta ttcgctcttt acgcccctcg ccaaccgcga caaccgcgca
     1981 gacttcgacg gcgaccagag gacggatatt tccatcttcc acaggggcac gggtcaatgg
     2041 ctctctcgca acagttccaa cggggttgat gtctcttata atcccccgtt cggacaaagc
     2101 ggcgacatcc cgacgcccgg cgattataac ggcgacggta agaccgaccg cgccatcttc
     2161 cgtccgggaa acggaacctg gtacatcgct acggacacga gcggagcgtt ctatacggtg
     2221 cagttcggcc agaccgcaga catccccgtg gcacgcgatt acgacggcga cggcagaacc
     2281 gatcaggctg tcttccgccc gggtataggc acctggtaca ttctcaacag cagtaacggc
     2341 acctggacgg ttgtgcagtt cgggcagagc ggcgaccgcg tcgtgccggg ttactacgac
     2401 actgacaaca aagccgacat cgccgtcttc cgtccctcca acgctacctg gtacatccgc
     2461 cacgcggacg gctcgtttca tggcgtgcag ttcggcgtcg ttagcgattg gccggtgcag
     2521 gccgattacg acggcgacgg tctgaccgac atcgccatct ttcgtcccgg cggcggggta
     2581 tggtggattc tgaggagttc taacaacacg gcttactccg tcccgtgggg cacaagcggc
     2641 gaccgtccgg tgccgggcga ctatgacggc gacgctaaag cggatatcgc cgtgcaacgc
     2701 cctgactcga acggcacctg gtacatcttg aaaagcacta ccggcactta cgacgccgtc
     2761 aacttcttct cggaataccc ggttcccgga ggatacctga cgccgctcta ctgatagccc
     2821 ttctcacaat gaagcgatag gcgggcgagg gagtatcttc cttagatgaa gatgctccct
     2881 cgcctggcta tcgcgtcgtt ggttccgctc aaagcagatg ccaagcgggc tgcccaaagc
     2941 ggcaagactt taacttgcca cttaatgggc gcgtccaacc agattcgcca caatcgctat
     3001 caactccgcc agttccaccg gcttcgagac gtgcatctcg aagcctgcac gcatagcgcg
     3061 caggcggtct tcagtccggg cgtaggccgt cagcgcgaca gccggagttc ttccgccagc
     3121 ccccggcggc aactccctca cccgccggat caactcgtag ccgtcctcgc ccggcatccc
     3181 gatgtcgctg atcagcacgt cgaacttcgc ggcggccaac gctgcaagcg cgtctcgcgc
     3241 tgacgaggcc atagtcacct gagcaccaca ctgccccagc ccgacggaaa gcaactcgcg
     3301 cgcgtccggt tcgtcatcga caaccaacgc cttcagaccg tccagccgct ccgggcaatc
     3361 gtgtgcgggc gacatatcgc gtgccgcctc aggcgtgcgc tcttccgtct cctctctgcg
     3421 gtggacggcg gcgattggca gcgtcaccgt aaacgttgcg cccgtccctt cacccgcgct
     3481 gttcgcacgg actgtgccgc cgtgcaactc catcaggtgg cgtacgatgg ctagccccag
     3541 acccagcccg ccgtgctggc gcgtcgtccg ctggtcggct tgccggaatc gctcgaagac
     3601 gtgcggcaga aactcgggtt cgatgcctgc gcccgtatcg ctgaccgcta tctcgatctg
     3661 cgaatttaca cgctccagtc tcacctgcac ccgcccgtcg cgcggcgtga acttgacggc
     3721 gttcgtcaag aggttccaga cgacctgctg gagccgggac gggtcgccca tgacggtcaa
     3781 tacccctgtg tcgatcacct tctgcaaacg cacccccttg gcctcggcag ccggacgcac
     3841 agcctcaacc gccgggtcga tgaacgagtg ggggctgacc ggcacaacat cgaggcgaag
     3901 attgcccgtg acgatgcgcg aaacgtccag aaggtcgttg atgagttgcg cctgcgagcg
     3961 tgcgttgcgt tcgatggtgt ccagtgccct gaccgcagcc tcgccttcga gttgcccgcc
     4021 gcgcagcagt tgcgcccaac cgaggatggc ggtcagcggc gtgcgcagtt cgtgcgagac
     4081 ggtggcgaga aactcgtcct tcaagcggtt ggcctcctgc gcctcccgca ggagtcgctc
     4141 gcgctcctct tctacacgct tgcgctctgt gatgtccgac gcctgaacga tgaagaactc
     4201 gacgctcccg tccacagcct tgaccgccgt gatcgaagcg tctgcgacgc gtgtgccgtc
     4261 agcggcctga tatgagtcca cgctgaagac gggcgtatcc gagcgcgcgg cagaggccag
     4321 gcgtgcaggc cagcccgcct gcatttccgt caggccatcc caccagggcg tctcccagaa
     4381 aagtcgcccc agcaccccct cgcgcgtgac accagcggcg cgcagcggca gttcgttgat
     4441 gtcgagcagg aagccttccg gcgagaggat cgccatgaac tgaaactgct ggttgaagat
     4501 gaggcggaaa cgcttttcgc tttcgcgcaa cgcttcttcg gcctgcttgc ggcgcgtgag
     4561 atcgcgtacg acggcttgca ccgcaggtcg gccttgatag agcagagggg cggaggccag
     4621 ttccccgtag atttccgtcc cgtcgagccg gatgaacttg atctccgaag ggggcggcgt
     4681 ctccccccgg ctcaccctct cgattcgctc gcggactaac ccgtgatagt ccgggtggat
     4741 caaactcgca attgatctgc cgacgatctg ttcgggactt tcggcgtgca ggagtctcgc
     4801 cccggcggtg ttcatgaaga gcaccacccc tccggcgtgg acggcgatgc cgtccggcga
     4861 gagttccacg agtcgctgga agcgctcctc ggattcgcgc agccgctcct ccgtcagctt
     4921 agcctcggta ttgtcgcgca gtatcttggc aaacccgcgt gggctgtcct gcccgtcgcg
     4981 gagcagcatc agcacgccct cagcccagaa gcgagagcca tcacgccgca cgtgccagcg
     5041 tttgtcctct gcgcgtccat ccctgagcgc cgtctccaac tccaacaggt gcgcgccctg
     5101 ctcatggtct tccggcgtga agatgatcga cgcgaactcg ccgacccatt ccccttcgcc
     5161 gtacccgagc aggctctcga cgcccggatt ccaactgatc gcctgcccct ccagattcat
     5221 cacgaagacg gcatagtcgc ggatgttctc cacgatcagg cggaagagtt catggctctc
     5281 aagagaagtt tctttcgtca tccgtatcgc ctggacaaga gccgttcaat gtcgcctcac
     5341 acggcgcagc gggggtgaat cgcttcgagg aacccgaact cgccgggctg tctgaaacac
     5401 tccaacgctc cggcggcgcg cgcctcgtcc ctccggtttc cctcgcagga cagtcctacc
     5461 gggcacactt cccctccgcg aaatcccgaa atggaggact ggctggttgg gggtttacga
     5521 actccagact aaagcctttc ggcgtcgcag tctaggccag aagagtgtct gcgccaccac
     5581 ccacgtctgt ctcctatcat ctactcgaca cgcgggcgga agataagcta tgaatgagga
     5641 atgaagcggc aggcacacga aagagtcgaa gcgacggatg ttccttcctc cgggcggcgt
     5701 gcgcgtttgc acgagatcat tttcgaggcg gatacgcgtg ccggtcgcgt cttcgacttc
     5761 gcgctcatct ggctgattct gctgagcgtc gccacggtta tactggaaag cgtccgctac
     5821 gtcagagtgc aatacgggga gctactctac gcactcgaat ggctcttcac actgctcttc
     5881 actatcgaat acatattgcg tctgctgagc gtgcgccgtc ccctgcgcta cgcgaccagt
     5941 ttcttcggcg tcgttgatct gctcgccatt atcccgacct atctcagcat cttcgtgccg
     6001 ggtagccatt acctgctggt catacgcata ctgcgcctgc tacgcatctt ccgcctgctg
     6061 aaactcgccg agtacatcac ggaagccgcc acgctcaggc gcgcgctgcg tgcgagtcaa
     6121 cgcaagatca gcgtctttct ctccgtcgtg gtgctgctcg tcatcatcat cggcgcgctg
     6181 atgtacgtcg tcgaaggcga agagcacggt ttcacgagca tccccgtcag catctattgg
     6241 gcggtggtga ctctcacgac cgtcggctac ggcgatctct cgccgcgcac gccgttcggt
     6301 cagatgatcg cttcaatcgt gatgattatc ggctacggca ttattgccgt cccgaccggc
     6361 atcgtctcgg ttgaactcgc gcaagccgcg cgagaacaga acgtctcaag gcagtcgtgc
     6421 cccgcgtgcg gagccgaggg gcacgacccg gacgccttct gctgcaagta ttgcggcgcg
     6481 aagttatgaa cggataattg ccgcgccgca cgaagcctta ctcaagcccg ctttattcta
     6541 aatctacccg ctgaaatcta cccgctgacg aattattcca tctgcaaggc tttggcttcg
     6601 tctgtgctgt cataagcgtt atgcagttgc accggcgcag ttttcgcttt gcgcaggcgc
     6661 agattcaaaa tttcgacgag gacggaaaac gccatcgcaa aatagatgta acctttcggg
     6721 atgtgctggt ctaagccttc ggcgatcagc gtgacgccga tcaggatcag gaacgacagg
     6781 gcgagcatct tgatcgtcgg gtgtttttgg acaaacgccc cgatgctgcc cgcgaaagcc
     6841 atcataaaaa tgatggacac gacgaccgcc gcgatcatga tccagagatt atcaaccatc
     6901 cccaccgccg tgatcaccga atcgagcgag aagacgatgt cgagcagggc aatctggatc
     6961 agaacgccga caaaagaggg ataaattttc gcggacttat gaccttcttc gccttcgagc
     7021 gagccgtgaa tttcatgcgt gcttttcgcc agcagaaaca gaccgccgag gagcaagacc
     7081 agatcgcgtc ccgatatttc ctgcccgaag actgcaaata aaggcgcggt taaaccgatc
     7141 acccacgtca gagaaaagag caggatcacg cgcatcatga gcgccagccc taatccgatg
     7201 taacgcgctc tcgcctgttg ttcgggcggc aatttgcctg ccagaatcga gataaaaacg
     7261 atattgtcaa taccgaggac gagttccaaa accgtcagag ttaaaaacgc gatccaggtt
     7321 tgcgggtcag ccatccattc catatcttct aaactctcct ttactcaatt gatgagaaac
     7381 tctctccaaa tcatatctcc gctatcatcc catagaaaca tctcacatcc atgtgcctga
     7441 tacttgtcaa cactcgtctc tccattaaaa ttttgcgtga gtactaaacg gaggaagacg
     7501 ggcggcgagg agtctgcgac tgccaccgga ggctgaatgg tctggagcaa acactggact
     7561 gaacatttac cctgcctcaa acttctctga taggtgcggg acttgccgtg gcacgctaca
     7621 ggattggtca ggtaacaagt caggcgaaat ccttctcgaa cctctttcag atcgcggcat
     7681 ataaggattc gaccatacga tctttcgggt agtgttgcaa aagtttcctt gcagcaaaaa
     7741 atattttgaa agctgaactg cctcgcgcgc gggcgagaga tgcctctcgc ctttcctgcc
     7801 tctccctccc gttgtcggac aggtaacgcg gaaccggctc cttgcaagac ccgaaccggc
     7861 tctggttttt gcccgtcaaa aggatgatac gggtgggtga tctcgatgga tgtgggagac
     7921 gccactgtag cgccggtcag gcgttgacgt agaatacata cataactgga cgagcaggta
     7981 cgggttgaat atgttgatct tcaacaccag agaattctaa ctgttcggcg cgctgctccg
     8041 tcgtttcaat tcctgattca caggccaacg gtcagcaagg agcgtctacg tgccgcgtgt
     8101 gagtttaata ataccgacgc attctcgccc gcagctactc gtgcgggcgg tggaaagcgc
     8161 gaagctcgca gggcgcgacg tggaactgct ggttgtggac gacgcttcga cggacggcac
     8221 ggcggaagtt tgccgcgcgc tcgaaggcat cagatacatc cggctcgaac acaatctggg
     8281 cgtagcaggg gcgcgcaacg cgggcatact ggcgagttcc gccgaatata tagcctttct
     8341 cgacgacgac gacctgcgcc tgcctgcctc gctcgacttg caactcgccg cgcttgcccg
     8401 ccagccggag gccggtttcg tatgcggttc gatgctgctg gcggatcagg aggggcgtct
     8461 gaccggggag aagtctgccc cggtccaccc cggcggagat ctcttctggg agcttatgga
     8521 actggccttt cccgtcatgc cgatgtcagt gttatttcgc agagagtgct tcttccgcgc
     8581 agggctgttc cgtaacaggc tggcaggcat tgacgactgg gatattttcg tccgcatcgc
     8641 cgaattgtat cccgtagttg ttatcagtga agcggtgggc atctaccggc agcccacgcc
     8701 ctcctccggt cagggttcgt cccaactggc gcgccacctg acgagagcgg tcagccacca
     8761 gagggaactg ctgcgcctgc cgcgcgcgat ggcggcttca gccgaacggc gcaggcaggc
     8821 tcggagggct gctataaatc gcgtcgccaa cacgctgctg cggaatgccg ccgaggggtt
     8881 gcccacaggc tcgtacgggt ttgtctgtgc taacatcctg agcgccctgc gactcgatcc
     8941 tttatacgtg gcacgccccg gagtttataa aaagctcttc aggcgattgc ttgcaacgta
     9001 ggcgtatgac gaggtcaggc gcctttctga cggacgcccg gaagcaggcc aactcgtaac
     9061 agtccttgca gtgcgcggag aattgatgcc ggaaatcagc ctgatcatca ccacacataa
     9121 ccgcccgcag atgctcgcac gcgccgtcga gagcgcgcag aaggccacgc ggagcgaggt
     9181 tgaagtggtg gtggttgatg acgcttcgac agatgaaaca gcccggctct gccgcagcat
     9241 cccgcatatc acctacgtgc gtgcggaacg caaccagaga gtcgcgggcg cgcgcaattt
     9301 aggcatcctc gccagccggg gcgagtttct cagttttctc gacgacgacg acctgaggct
     9361 gcccggctcg ctcgacccgc agttggccgc gctggcttcc gacccgcgag cgggcttcat
     9421 ttacgggcag gcgatcctga gcgggcgtga gggcgcagca gaggaagacc gctacccggc
     9481 gtgctgtccg cagggagata ttttcttccg gctgctggta caaaacttca tcccctgcgg
     9541 ctcggtagtt tttcgacgcg cctgcttgtc tcgcgtcggc ctgctcgatg atgcattggc
     9601 cgggattgac gattgggatt tatggctgcg catcgccgcg ctctacccgg tgatagctgt
     9661 ggagcaaccg gtcgtgcgct ggcgcaaatc cacgccggtt tccaggcagg gcagttcgcg
     9721 tgcgttggag atggttagtc ttagcacggg gcaattccat aaaagctggc tggcgttatc
     9781 gcgtgtcgct gagactccgg cctcgcagcg gcgagagtgc tcacgccgct tctcggaaaa
     9841 tatggccggt catctgctct gggaaacatg gcgggcgttt gccacgggag aggtgacggc
     9901 ggcctgccgc aatatgctcg gcgcgatgcg tcttcatccg gtggcaatga tacgtgtggc
     9961 agcgcgccgc acgagtttac gcttcttttt gactcatgcg cccggcgagt ggcgtgacct
    10021 taaagcccgc acggggcaat taccaaactc ttagaacaat gctatctgat gaagataatt
    10081 ctcggcaaag gccaattcat ggggccggtg agcggtgcgg acgaaacgct cgttgcctac
    10141 gccactcagc tacgtttagc cgggcactcc gtctcagtcc tactgatgta cccgcacatg
    10201 gccggggatc aatacttcca acgcctctcg aaggcggacg tgcccgtctt ctccatcgcc
    10261 cccgccagcg tccgcgcata tctggatgcg ggacgtaatc tgttgcgggg tctattgcag
    10321 gtttttccca tgccgaggcg gctcctgcga agcagggcac aacggcttgc cagtagcgtg
    10381 gcggatcaat acttcgatca gtgctgtgat tacttaagga gactgaacgc agatctggta
    10441 cacgtcatca cgcccgatcc gagcgcgatg atcatgattc gcgcggcaag cgcagtcgga
    10501 ctcccggtct tctatcaaga gctggggata ccctatcacc cgccggagtt cgagtcgtat
    10561 tacaaagagt tcacttccgt cttgcccctg tgctcggaag tggccgcact ctcaccgcaa
    10621 ctcgcgctcc attgccgcga gaaactgcca cacgccaact ctctctccgt gcttcccatc
    10681 atggcgagcg caccttttca tcaggtgacg acttccgcga aagccccgcc cacacagccg
    10741 actgcgggcg tcacgtttgg cttcgccgcc cggttcgaat atttaaaagc cccacacgtt
    10801 ctgctggagg ctttcgccag agtctaccgg gagcgcgacg atgtgaggtt gaggctcgtg
    10861 ggggtcggct cgctcagacg taagatcatg gcgcgcgccg ccgctctcgg cgtggcgcac
    10921 gcgtgcgact ttcctccggt atatacgcaa cttgaagaga agggcgcttt catgcggagt
    10981 ctgaacgtct tcgtgttgcc atccctcacg gagggcacgc ccaacagcat tatggaagcg
    11041 atggcacacg gcctgcccgt catctcgacg acggtgggcg ggattcccga tgtcgtcaca
    11101 gccgaaacgg gcatcctcgt cccgcccgaa gacacggagg ctctcgcagg ggcgatgctc
    11161 cgactggcgg gcgacccggc cttgcgtgcc cgcatgagtg aggcggccaa attcaggtat
    11221 gaagaactct tctccccgga ctctgtcttg cctgtggtga tgaacaggta tcgagcggtt
    11281 atctccggca ggaacaatca tcaaccggac gataagccgg aaagcaacgg actctcgcac
    11341 ccctgggcaa acacatattt ctaaaggtgg cgggcgaagg ttgaataaag aggcagggcg
    11401 ttatatggaa tatacgggtg agagatttat gcctcacctc ttgggcgaga tcaaatacga
    11461 acacctgcac cggtatgcgc ttgcgcaaga attcgtgaag ggcaaggagg tgttggacat
    11521 cgcctgcggg gagggctacg gcgcggcttt gatgtcggct gcggcgcgca gcgtcgtcgg
    11581 cgtggacatt gatgcggcgg tcatcgaaca tgcccgccgg aagtatgccg cccacccgaa
    11641 tctggtattc cgcaccggct catgcgactc gatcccgctg cccgaccgga gcgtcgaggt
    11701 cgtcacctct ttcgaaacca tcgagcatca cgatcagcat caggaaatga tgcgggaagt
    11761 cagaagggta ctgcgccccg gaggggttct catcatctcc tctcccaacc gcccggtcta
    11821 ctccgaactg tacggcagct cgaatcagtt tcacgtcaag gaactcgatt atcaggagtt
    11881 tacggattta ctgaaagagc atttccatca catcgcggtc tacgggcaaa agctcgaagc
    11941 cggttcattc gtcttttctg tagatgacac gccggggcgt gacttcacgg cttactccgg
    12001 caagggcgag caactgcgcg gcgcactccc gcccctcagt gcgccatcct atttcatcgc
    12061 catctgctct gacgcggagt tgggaatcgc gccgggtttg gagtcgatct acctcgacca
    12121 tggagactcc ctacaggagc acgcgctgga ggagttacgc cgtctgagag ccgaccacca
    12181 gcaggccgtc gaactttacc tccggcacgc actcgaactc gaagagatca agtctcatat
    12241 cagctttaag ctcatctcgc gtcttctctg gcctatgacc gggaaactct tgccgcaaag
    12301 tattcgctct cgtaccggaa aaaacctgtc gcggatgaag cgcacgcccg ccgcgctcgc
    12361 cctcgcccgg aaagccgaaa aactgcgctt tgagattaac gaaaatattt ctcagccatt
    12421 cgtcatcggc aaagggaacc tctactacct gagcggctgg tgctaccacc cgcgccacag
    12481 ccttcggagg ttatacgtgc tggttgacga cgagccgcac gaagtcttca accattcgct
    12541 ggcggaaacg gaagcatgcc gcgaccattc atccgaccac gaattgtctg gcaactgtct
    12601 gacgagtcgc ttctggactg cccttccatt ctcccaactc gacgcgccgc gcgaagttaa
    12661 actttctctg cgcgccatcc tgagcgacga aagtgtaagc gaggccaaac tcggaactct
    12721 ccgtttgctg ccgtcgcctc cgccggagtc gcgtgcggag tggaacgagt cggcagatgt
    12781 cgcttcgtcg tggcctgtgg cgggagacgg ggcgcgcgtc gtcatctgca tggcgacaca
    12841 tcaaccgccg cttgatctct tcgcactcca gataaagagc ctgatcgcgc agtctcaccg
    12901 gaactggctc tgcctcatca gtgacgattg ctccgagcct g
//
LOCUS       DASWNS010000523         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_409052, whole genome shotgun sequence.
ACCESSION   DASWNS010000523 DASWNS010000000
VERSION     DASWNS010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409052"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VGB76_17995"
     CDS             <1..1488
                     /locus_tag="VGB76_17995"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEY0100844.1"
                     /translation="FEQEATSGNVVPVVRRVLADLQTPVSAFLRLANEAKHAFLLESV
                     EGGERVARYSFLGVEPEMIVRGRGRETFIERPDGATEHLRGVRATDFLRDYFRRHKLA
                     RRDQLSPFSGGAVGYLAYDAVRWFEPVLDAGGAEQESDEAVLMFFRTTLAFDRVRQQI
                     EISSIVFTGEANDSREGLRRLYAQAVRRTETIENALQENAPAQKAARRAETGAEEESQ
                     PQFVSNWKREDFERGVETIKEHIAAGDCYQAVLSQRFSTQVKASPLEIYRALRATNPS
                     PYMYFLRLGEESIVGASPEMLVRCRGAELEYRPIAGTRPRGANEAEDARLGVELRADA
                     KEVAEHLMLVDLGRNDLGRVAAFGSVEVKELMSVERYSHVQHLVTSLTARLNDSADCF
                     DALAACFPAGTVTGAPKVRAMQIIDALEPDRRGLYAGAVLYLDYAGNLDSCIAIRTVR
                     LRGGRAEVQAGAGVVADSVPAREYEETVNKARAMLRAIEMAERGL"
     gene            1594..2364
                     /locus_tag="VGB76_18000"
     CDS             1594..2364
                     /locus_tag="VGB76_18000"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0100845.1"
                     /translation="MLAASANCVRQPSQRASPETARRELAMRGIDFKEEEFVRHAKDG
                     DAWAVRLFLAAGMSPDVRDARGSRPLTAALNGSQKGIAKILLERNADPNVLDRDGKTP
                     LIVAARAADTELVRLLLDRRADVNMRALENYTALSYSMANADPEIAEILIERGADVNA
                     TDDSGGTPLSWAAFYGRASLVEKLLAKGADPNAVNKIGGTPLMIAAAKGNAEIVRLLI
                     ASGANPKAKDKRGRTARQFALDSYHTEVAELLAQAEKK"
     gene            2452..>2712
                     /locus_tag="VGB76_18005"
     CDS             2452..>2712
                     /locus_tag="VGB76_18005"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HEY0100846.1"
                     /translation="MIFVIDNYDSFTYNLVQYLGELGAEMSVRRNDEVTVEEIERDYR
                     PDKILISPGPGEPKDAGISCAAIEHFAGRIPVFGVCLGHQAIG"
BASE COUNT          523 a          752 c          940 g          497 t
ORIGIN      
        1 ttcgaacagg aggcgacgag tggaaacgtc gtgcccgtgg tgcgccgcgt gctggcggat
       61 ttgcagacgc ctgtgagcgc gtttttgcgt ctggcgaatg aggcgaagca tgcgtttctg
      121 ctcgaatcgg ttgagggcgg cgagcgcgtg gcgcgctatt cgtttctcgg cgtcgagccg
      181 gagatgatcg tgcgcgggcg cgggcgggaa acttttatcg aacgcccgga cggcgcgacc
      241 gagcacctgc gcggcgtgcg cgcgacggat ttcctgcgcg actatttccg gcggcacaaa
      301 ttggcgcggc gagatcagtt gtcgcccttt tccggcgggg cggtcggcta tctcgcttat
      361 gatgcggtgc gctggttcga gcctgtgctc gacgcgggcg gcgcagagca ggagagcgat
      421 gaagccgtcc tgatgttttt tcgcacgacg ctcgcttttg atcgcgtgcg acaacagatt
      481 gaaatcagtt cgatagtttt caccggagag gcaaacgaca gccgcgaagg gttgcgcaga
      541 ctttacgcgc aggccgtgcg ccgcactgag acaatcgaga acgctttgca ggagaacgcg
      601 cctgcgcaaa aggcggcgcg gcgggcggag acgggggcgg aagaagagag tcagccgcag
      661 tttgtttcaa actggaagcg agaggatttc gagcgcgggg tggagacgat caaagagcac
      721 atcgcggcgg gcgactgtta tcaggccgtg ttgtcgcagc gtttcagcac gcaggtcaag
      781 gcgtcgccgc tggaaattta tcgcgcgctg cgtgcgacga acccttcgcc ctacatgtat
      841 tttttgcggc ttggcgagga atcaatagtc ggcgcgtcgc ccgaaatgct ggtgcgttgt
      901 cgcggggcgg aacttgagta tcgcccgatt gcagggacgc gcccgcgcgg cgcgaacgaa
      961 gcggaagacg cgcggctcgg cgtggagttg cgcgcggacg cgaaggaagt ggcggagcat
     1021 ctcatgctcg tcgatctcgg tcgcaacgat ctggggcgcg tcgccgcgtt cggttcggtg
     1081 gaagtcaaag agctgatgtc ggtcgaacgc tattcgcatg tgcaacatct ggtgacgagt
     1141 ctgacggcgc gtctgaacga ctcggcggac tgtttcgacg cgctggccgc ctgttttccg
     1201 gcgggcacgg tgacgggtgc gccgaaggtg cgcgccatgc agatcataga cgcgctcgaa
     1261 cccgaccggc gcggcctcta cgcaggcgcg gtgctctatc tcgactacgc gggcaatctc
     1321 gattcgtgta tcgctatacg caccgttcgc ctgcgcggcg ggcgcgcgga ggtgcaggcg
     1381 ggcgcgggcg tcgtggccga ttccgtgccc gcgcgcgaat acgaagagac ggtcaacaag
     1441 gcgcgagcca tgttgcgtgc gattgagatg gccgagcgcg gcctgtgaac ggatcatcgc
     1501 tttgaagttg aaataaagcg ggcagggaat aaagagttga gcaacgtggg gagatattta
     1561 tcgcgagcga cgcctctttt agtcgcagtc tcggtgttgg ccgcgagtgc gaactgtgtg
     1621 cgccagccga gccagcgtgc gagtcctgag acggcgcggc gcgaactggc tatgcgcggg
     1681 atcgatttca aagaggaaga gtttgtgcgc cacgcgaagg acggcgacgc gtgggcggtc
     1741 aggttgtttc tcgcggcggg catgagtccg gacgtgcgag acgcgcgtgg aagcaggccg
     1801 ctcacggcgg ctctgaacgg ttcgcagaag ggcatcgcca aaattctgct cgaacgaaac
     1861 gccgacccga acgtgctcga ccgcgatggc aagacgcctc tcatcgtcgc cgcgcgagcc
     1921 gcagatacgg aactcgtgcg cctcctgctc gaccgtcgcg ccgacgtgaa catgcgcgcg
     1981 ctggaaaact acactgcgct ctcctattcg atggcgaacg ccgatccgga gatcgcggaa
     2041 atcctgatcg aacgcggcgc agacgtgaac gcgacggacg attcgggcgg cacgcccctc
     2101 tcgtgggcag ccttctacgg gcgtgcgtcc ctcgtagaga aattgctggc gaagggcgca
     2161 gacccgaacg ccgtcaacaa aatcggcggc acgccgttga tgatcgccgc cgcgaagggc
     2221 aacgcggaga tcgttcgcct gctcatcgcg agcggcgcaa atcccaaagc gaaagacaaa
     2281 cgcggaagga cggccagaca gtttgccctc gacagttatc atacggaagt ggccgagttg
     2341 ctggcgcagg cggagaagaa gtgacgagtt gttgactgtc aacttgcagc acaaccactc
     2401 tccgcgccta tctcaaactg tcagccagct actaacagct agcagccgat tatgatcttc
     2461 gtgatagata actacgattc tttcacgtac aacctcgtgc aatatctggg cgagttgggt
     2521 gcggagatgt cggtgcgtcg taatgacgag gtgacggtgg aagagatcga gcgtgattat
     2581 cgcccggaca aaatcttgat ctcgccgggg ccgggcgagc cgaaagacgc gggcatttcg
     2641 tgtgcggcca tagagcattt cgcgggacgg attcccgtct tcggcgtctg cctcggccat
     2701 caggccatcg gc
//
LOCUS       DASWNS010000524         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_382802, whole genome shotgun sequence.
ACCESSION   DASWNS010000524 DASWNS010000000
VERSION     DASWNS010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382802"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VGB76_18010"
     CDS             <1..1372
                     /locus_tag="VGB76_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100847.1"
                     /translation="ASVFRVGVDGALPLPTLAAASVPFIPAQPFGETLSFQIDPDFDA
                     GENYSFDVTVQRELPRGLVLELGWIARLGRKLPASVNFNSSPYFQLDARSGQTFAQAF
                     DAVAGLLRAGTPAANIPAQAWFQNNMPGGTARVITGNEANFINGNVSNIFQTIDLARL
                     ANNLTPFNNLQSQTAFVRTSIGRSNYHALALILRKRLARDLVFDFNYTFSKSLDQTSV
                     TQNSTNAMQNAFAPDFDYGPSDFDRTHNFNGYFLYELPAGRGRRFDAGRVLDKFIGGW
                     SLAGIFRAASGLPLIVSQGTSALGGGSTLVAPSGAIPLSAPGSLRTGVNAGVNGSNNV
                     GTNGAPASGGSGINLFNDPEAAFNSFRRILLSQDGRSGRANPLRGFSMWNLDLSLAKR
                     TPLTERVAARFSLDFFNLFNHPLFSNPALDLNNSRSFGVVTQQNVPTRRESSSRWIQF
                     GLRLEF"
     gene            complement(1498..>2922)
                     /locus_tag="VGB76_18015"
     CDS             complement(1498..>2922)
                     /locus_tag="VGB76_18015"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0100848.1"
                     /translation="EASLEVPASTMTCANCHGFDGQGKPEGGITPSNITWEALTKSYG
                     VTHASGRKHPPYTERAVELALTRGLDPAGNRLLNVMPRYGMSREDMNDLVAYLKRLGT
                     DRDPGLTDREIRIGMLVAAKGALAEMGQAIKAVVAAYFAEVNSQGGIYNRRIELKFAE
                     TGDTPAATLASVERLVKDEQVFALSSAMIAGADREIATLAHNAEVPLVAPFTLYPPLA
                     APVNRQVFYLLSGIDDQARALVLFAAQKFPDKNATRAAIVYPESEVNAGVAVAIDEQS
                     RLAGWSAPGRVKYARGSFDAATTAKELSAKGVNAVFLLGGSDDARALWTAAEKLNWSP
                     TLYLPGAMTGRDILDAPQAFREKIFLSFPSVPADIKPPALENFRALAAKYKLPQGHIA
                     AQVSAYVSAQLLVEGLKRAGRDLSREKLIGALEGLYEYETGLTPRLTYSPNRRVGAAG
                     AYIVAVDMEKKQFLPASGWIGIKP"
BASE COUNT          497 a          992 c          859 g          574 t
ORIGIN      
        1 cgcctccgtc tttcgcgtgg gcgttgacgg cgcgctgccc ctgcccacgc tggcggcggc
       61 gagcgtcccc ttcatccctg cgcagccgtt cggcgaaacc ctctcctttc aaatcgaccc
      121 cgacttcgac gcgggcgaaa actacagctt cgacgtgacc gtccaacgcg aactcccgcg
      181 cggcctcgtg ctcgaactcg gttggatcgc tcgtctggga cgcaagctcc cggccagcgt
      241 caacttcaac tcgtcgccct atttccaact cgacgcgcgt tccggtcaga ccttcgcgca
      301 ggctttcgac gccgtcgcag gactgttgcg cgccggaacg cccgccgcga atattccggc
      361 gcaggcgtgg tttcaaaaca acatgcccgg cggcacggcg cgcgtcatca cgggcaacga
      421 agcgaacttc atcaacggca acgtctcaaa catcttccag acgatagacc tcgcgcggct
      481 ggcaaacaat ttgaccccct tcaacaacct gcaaagccag acggccttcg tccgcaccag
      541 catcggacgc tccaactatc acgcgctcgc gctcatcctg cgcaagcgtc tcgcgcgcga
      601 tctcgtgttc gacttcaact acaccttctc gaagtcgctc gatcaaacca gcgtcacgca
      661 gaactccacc aacgccatgc agaacgcttt cgctccagac ttcgactacg gcccttccga
      721 cttcgaccgc acgcacaact tcaacggcta ttttctctac gaactccccg ccggtcgtgg
      781 ccgccgtttc gacgccgggc gcgtgctcga taagttcatc ggcggctggt cgctcgcggg
      841 catcttccgc gccgcttcgg ggctgccgct catcgtctcg caaggcacga gcgcgctcgg
      901 cggcggctcc acactcgtcg ctccgagcgg cgcgattccg ctctccgctc ccggcagttt
      961 gaggacggga gtaaacgccg gggtcaacgg ttcgaataac gtcggcacga acggcgcgcc
     1021 cgcgagcggc ggttcgggca tcaacctctt caacgacccg gaagcggcct tcaacagttt
     1081 cagaagaatt ttattgagtc aggacgggcg ttccggcagg gcgaaccccc tgcgcggctt
     1141 ttcgatgtgg aacttagacc tctcgcttgc caagaggaca ccgcttacag aacgggtcgc
     1201 ggcgcgcttc tcgctcgact tcttcaacct cttcaaccat ccgctcttct ccaaccccgc
     1261 gctcgacctg aacaattcgc gcagcttcgg agtcgtcacg caacagaacg tcccgacccg
     1321 ccgcgaatct tcaagccgct ggattcagtt cggcctgcgc cttgaatttt agaaagggag
     1381 ggatgaaggg atgaagggat gagggatgag ggatgaggga tgaataagaa aagcattcaa
     1441 ctctcttgag tgctgtctct tattcatccc tcatccctca tccctcatcc cttttttcta
     1501 cggcttgatg ccgatccagc cgctcgccgg gagaaactgt ttcttctcca tgtcaacggc
     1561 gacgatgtac gcgcccgccg cgccgacgcg acggttcggg ctgtaggtga gacgcggcgt
     1621 gagtccggtt tcgtattcgt agagtccttc gagcgcgccg atcaacttct cgcggctcag
     1681 gtcgcgcccc gcgcgtttca atccttcgac cagcagttgc gccgagacgt aggccgaaac
     1741 ctgcgcggcg atgtggcctt gcggcaactt gtacttggcg gcgagcgcgc ggaagttctc
     1801 tagcgcgggc ggcttgatgt cggcgggcac gctcggaaac gagagaaaga tcttctctct
     1861 gaacgcttgc ggcgcgtcga gaatatccct gcccgtcatc gcgccgggca ggtagagcgt
     1921 gggcgaccag ttcaactttt ccgccgccgt ccacagcgcg cgcgcgtcgt cgctcccgcc
     1981 gagcaggaag accgcattca cgcctttcgc actcaactct ttagccgtag tcgccgcgtc
     2041 gaaacttccc cgcgcatatt tcacgcgccc cggcgcgctc catcccgcga gcctgctctg
     2101 ttcgtcaatc gccacggcca cgcccgcgtt cacttcgctc tccggataga cgatggccgc
     2161 gcgcgtcgcg ttcttgtcgg ggaacttctg cgcggcgaag aggacgagcg cgcgagcctg
     2221 atcgtctatg ccggagagca ggtagaagac ctgacggttg acgggcgcgg cgagcggcgg
     2281 gtagagcgtg aagggggcga cgagcggcac ttccgcgttg tgcgcgagcg tcgcgatctc
     2341 cctgtcggct ccggcgatca tcgcgctcga cagcgcgaag acctgttcgt ctttgacgag
     2401 acgctcgacg ctcgccagcg tcgccgccgg agtgtcgccc gtctccgcga acttcaactc
     2461 gatcctgcga ttgtaaattc cgccctgact gttgacctcc gcgaagtagg ccgcgaccac
     2521 ggccttgatc gcctgtccca tctccgcgag cgcgcccttc gccgcgacga gcatgccgat
     2581 gcgtatctcc ctgtcggtca aacccggatc gcggtctgtg ccgagtcgct tcaggtaggc
     2641 gacgagatcg ttcatgtctt cgcgcgacat cccgtagcgc ggcatgacgt tcaagaggcg
     2701 attgcccgcc gggtcgaggc cgcgcgtgag ggcgagttcg acggcgcgtt cggtgtaagg
     2761 cgggtgcttg cgcccgctcg catgcgtgac gccgtaggat ttcgtgaggg cttcccaggt
     2821 gatgttcgag ggcgtgatgc cgccttcggg tttgccctgc ccgtcgaagc cgtgacagtt
     2881 ggcgcacgtc atcgtgctgg caggcacttc gagcgacgct tc
//
LOCUS       DASWNS010000525         5185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_253615, whole genome shotgun sequence.
ACCESSION   DASWNS010000525 DASWNS010000000
VERSION     DASWNS010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253615"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..600)
                     /locus_tag="VGB76_18020"
     CDS             complement(4..600)
                     /locus_tag="VGB76_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100849.1"
                     /translation="MKGAEGTQMITTRQGRAAQLGSTSRTVLVRCTCGRRNYFDRQAW
                     QWLEVAFCVRCWQAILCFDLRVVSRWEGERMLREHVVYAGELKALRTIEGEMRRFLML
                     FRAEPLWTWPPQVVRMAQAVAANLQLVEAARARQGGVAGVPLEEEPAEMGVFLELPGE
                     EAYELTPEQTRLLSFFSRLSAERRAVMLDFIAQEGSEL"
     gene            complement(597..968)
                     /locus_tag="VGB76_18025"
     CDS             complement(597..968)
                     /locus_tag="VGB76_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100850.1"
                     /translation="MIAPSLYAYLRMRGVRLSLATGSRLQVHAPVGAITVYMQSFINQ
                     HAAELAQFIFELEECAAILEFEQGHTRAEAERLARLCVWGGMAAPNGQLWLKAYASTH
                     PLVQQVLSRFRAEVIEVKLVA"
     gene            complement(965..2785)
                     /locus_tag="VGB76_18030"
     CDS             complement(965..2785)
                     /locus_tag="VGB76_18030"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEY0100851.1"
                     /translation="MSEQLPPPPAYVPTAEDEYAFRRRRTHAHIEAMSGLILQAIAYP
                     TRRHRDFICALQSANSGNDAKVPYTPFRRAHLTLADYMKYDGSPETRRKAVYREICCL
                     RRFQRTTGIVIFHVTPGNEEEATEYIDYLTPLADAAMQRALSSPIWKTDKAEAKRQAV
                     QWAVSMLPRIPIEEDEEAESKELSLHDYERTQEERFYQSIERTADEIEKRGGDHRWIE
                     RVAHELLRIASSRKKTAGARRSDAKLRAAAESAAAVEGGTADESGDLYNVGQLHPVTE
                     SDTSMRGEDRSVLPPPSAATVADQDEETGDGQGGRTDLSSPPAPDHLEAALTYARRGW
                     HVFPLHNPVEGGACSCSQSKACRSMGKHPRTRQGLKDATTNEQQVRAWWHRWPQANVG
                     LAMGKISGLVAIDVDPRSGGDASLAELFDAHGTFPATLEALTGGGGFHFLFAHPGVKF
                     KNSSSVLGEGLDIKTDGGYIVAAPSLHASGQRYEWRGTAEPSPLPSWLLTLLTKERAK
                     PESKAATAGATPRSGVTTNASGALIPAGERNKKLFRIACALRGDGSTHQQIVEELAAI
                     NSTRCAPPLDEGELRRIASSAARYAPEARRRVARAEGVTP"
     gene            complement(2785..3009)
                     /locus_tag="VGB76_18035"
     CDS             complement(2785..3009)
                     /locus_tag="VGB76_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100852.1"
                     /translation="MMNVESIEAIEPLPVAIRELGRRYAAGEITRERRDARLVELNKR
                     RPDARRQRARSLSLTAPAPVTFYSLDEAAA"
     gene            complement(3115..3564)
                     /locus_tag="VGB76_18040"
     CDS             complement(3115..3564)
                     /locus_tag="VGB76_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100853.1"
                     /translation="MDSWQINRIAVGNCAKAVCEALCKLGKRCSVRWVNRQCEPPQDV
                     TRSDYYGAFRRWFEAVFIANRDGAEFLFEDFCAFVQSMRDFEDLATADLQQQFARCEG
                     EHSDIIRVALLSGDTGQLQREIAEDIAEKRLLLAMIRAREIGQARAA"
     gene            complement(3674..3898)
                     /locus_tag="VGB76_18045"
     CDS             complement(3674..3898)
                     /locus_tag="VGB76_18045"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0100854.1"
                     /translation="MYRDDKIRAEMAIQQLHDDDMAAKTGLTRQTIALLRKGEAKDVK
                     LSTLDAVARALGKETPWLVEPRREPEVARA"
     gene            4019..4393
                     /locus_tag="VGB76_18050"
     CDS             4019..4393
                     /locus_tag="VGB76_18050"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0100855.1"
                     /translation="MSNEQETLGDFVRRVANEKGLSQREVARRSGGLISHVTVGDIIN
                     KNRGDVKTETLRGLAKGLGVTEEEVFAVARGKKPEDDPDFLKSRLGLLSLKFDKIPID
                     KRVNVEALIELLDREVERLTKK"
     gene            4390..4824
                     /locus_tag="VGB76_18055"
     CDS             4390..4824
                     /locus_tag="VGB76_18055"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEY0100856.1"
                     /translation="MNLALVALKAFPHWNRRIITERDIQRVCKKKGVYIVEGKVGVPG
                     LYIPYAGFAFIVIDPDLPPGMRLWVLLHELAHHLLHVPGLQLFDKRYKTKADFEANFI
                     AAIGAVTLEMVMTKLPGEIQEEYGYPKELTDLRKWIYDNYRI"
BASE COUNT          991 a         1569 c         1570 g         1055 t
ORIGIN      
        1 cgctcatagt tcactgccct cctgcgcgat gaagtccagc atgacggcac gccgctcagc
       61 agagagccgt gagaagaagg agagcaggcg cgtctgctcc ggcgtgagtt cgtacgcctc
      121 ttcacccggc aactccagaa agacgcccat ctccgccggc tcctcctcga gcgggacgcc
      181 agccactccg ccctgacgag cgcgcgccgc ttcgacgagt tgcagattag cggcgacggc
      241 ctgcgccatg cggacgacct gcggcggcca cgtccagagt ggctcggcgc ggaacagcat
      301 gaggaagcgg cgcatctccc cttcgatggt gcgcaaggct ttcaactctc cggcataaac
      361 tacatgctct ctgagcatcc gttctccttc ccagcgactg acgactcgta aatcgaaaca
      421 gaggatggct tgccagcagc ggacacagaa ggcgacctcg agccactgcc acgcctggcg
      481 gtcgaagtag ttgcgacggc cacaggtgca gcgcacgagc acggtgcgcg acgtgctgcc
      541 aagttgagct gcccgcccct gtctggtcgt gatcatctgt gtcccttcgg ctcccttcat
      601 gcgactaact tcacctcgat gacctctgct ctgaagcgcg agagcacttg ctgcacgagc
      661 ggatgagtgg aggcgtatgc cttcagccac agttgaccat tgggtgccgc cattccgccc
      721 cacacgcaca gcctggccag gcgttccgcc tctgctctgg tgtgcccctg ctcgaactca
      781 aggatggccg cgcactcctc gagttcgaag atgaactgcg ccaactccgc cgcatgctga
      841 ttgatgaagc tctgcatata gacggtgatg gcacccactg gcgcatgcac ctgcagccgg
      901 ctcccggtgg ccagcgagag cctgacgccg cgcattcgga gataagcgta gagcgatggc
      961 gcgatcatgg tgtgacaccc tccgctcgcg ctacacgtcg cctggcctcc ggtgcatagc
     1021 gcgccgcgct tgaggcgatg cggcgcaact caccttcgtc gagcggaggt gcacacctcg
     1081 tcgagttgat cgcagctaac tcttcgacga tctgttgatg agtcgagccg tcaccgcgaa
     1141 gcgcacaggc gatgcggaag agtttcttgt tccgctcgcc cgcggggatg agtgcgccgc
     1201 tggcgttcgt cgttacacca ctgcgcggcg tagcacctgc agtggcagct ttgctctccg
     1261 gcttcgcacg ctccttagtg agcagtgtga gtaaccagga aggcagcggc gacggctccg
     1321 cggtgcccct ccactcgtaa cgctggccac tggcatggag tgacggagcc gcgacgatgt
     1381 agccaccatc ggtcttgatg tcgagtcctt cgccgagcac tgaagatgag ttcttgaact
     1441 tcacgcccgg gtgcgcgaac aggaagtgga agcctccgcc tccagtgagc gcttcgagcg
     1501 tcgcggggaa cgtgccgtgc gcgtcgaaca actccgccag gctcgcgtct ccgccgctgc
     1561 gcggatcaac atctatggcg acgagtccgg agatcttccc catcgccaag cccacattgg
     1621 cctgtggcca ccggtgccac cacgctctca cctgctgctc gttcgtggtc gcgtctttca
     1681 gcccctgacg cgtcctgggg tgcttgccca tgctgcggca agccttgctc tggctgcagg
     1741 agcacgcgcc gccctcgacc ggattgtgca gcgggaagac gtgccagccg cggcgcgcat
     1801 aagtgagcgc agcctcaagg tggtcagggg caggggggga ggacagatct gtcctccccc
     1861 cctgcccgtc gccagtctct tcgtcctggt cggccacggt cgcagcagac ggagggggga
     1921 ggacagatct gtcctccccc ctcatcgaag tgtcactttc cgtaacagga tgaagttgtc
     1981 ccacgttgta tagatcacca ctctcgtctg ccgtcccccc ctcaactgcc gccgccgact
     2041 ccgccgccgc gcgcaacttt gcgtcagagc ggcgcgcgcc cgccgtcttc ttcctcgagg
     2101 atgcgatgcg caggagttcg tgcgccacgc gctcgatcca gcgatggtcg ccgccgcgct
     2161 tctcgatctc gtctgccgtg cgctcaatgg attgatagaa gcgctcctcc tgtgtgcgct
     2221 cataatcgtg tagggagagt tccttgctct cggcctcctc atcctcctcg atggggatgc
     2281 gcggtagcat cgagacggcc cactggacgg cctgacgctt ggcctcagcc ttatccgtct
     2341 tccagatcgg ggaggaaagg gcgcgctgca tggcggcatc ggcgagaggt gtgaggtaat
     2401 caatgtactc ggtagcttcc tcttcattgc ccggggtgac gtgaaagatg acgatgccgg
     2461 tggtgcgctg gaagcggcgc agacagcaga tctcacggta gacggccttg cggcgtgtct
     2521 ccggagagcc gtcatacttc atgtaatcgg cgagcgtgag gtgcgcgcgg cggaaggggg
     2581 tataaggaac tttggcatcg ttgccggagt tggcgctctg gagcgcgcag atgaagtcac
     2641 gatggcgacg ggtagggtaa gcgatggcct ggagtatcaa gccgctcata gcttcgatgt
     2701 gggcgtgcgt gcgccgccgg cggaaggcat actcgtcctc tgcggtcgga acatacgccg
     2761 ggggcggtgg tagctgctcg ctcatcaggc agccgcctcg tcgagtgaat agaaggtgac
     2821 gggagcggga gcggtgagac tgagtgatcg ggcacgctgg cggcgcgcgt ccggtcgccg
     2881 cttgttcaat tccacgaggc gagcatcgcg gcgctctctg gtgatctcgc ctgcagcgta
     2941 gcgtcgccca agttcgcgga tagcgacggg tagtggttcg atggcttcga tagattcgac
     3001 gttcatcatc gtatggttct ccggagcggg cgcgcccgag tgatcagtaa ggcgcgcccg
     3061 ccccttcaat tgcccttaca ggttgcagac cccgcgaagg caaactcatc aatttcaagc
     3121 ggcgcgcgcc tgtcctatct cgcgcgcccg aatcatcgcc agcaggagcc gcttctcggc
     3181 aatgtcctcg gcgatctcgc gctggagttg gccggtgtcg ccggagagga gcgctacgcg
     3241 gatgatgtcc gagtgctcac cttcgcagcg agcgaactgc tgctggaggt ctgccgttgc
     3301 caggtcttcg aagtcgcgca tggattggac gaaggcgcag aagtcttcga agaggaattc
     3361 agccccgtcc cggttagcga tgaagacggc ctcgaaccag cggcggaacg ctccgtagta
     3421 atccgagcgg gtgacatcct gcggcggctc gcactggcga ttgacccagc ggacggagca
     3481 gcgtttgccc agcttgcaca acgcttcgca gaccgctttg gcacagttgc cgacggcgat
     3541 gcggttgatc tgccatgagt ccatgtgtga ttcatcagcc gcgcccctct cgcagttggt
     3601 tcctagatag ttgaatgaag aactagcagc gcgccctccg catgccgcac aattgccgca
     3661 tggagcgcgc gcctcatgct cttgctacct caggctctct cctcggctcg acgagccacg
     3721 gcgtttcttt gccgagcgcc cgggcgacgg catccagagt ggagagtttc acgtctttag
     3781 cctcaccctt ccgaagtaat gcaatggtct gccgggtgag gccagtcttt gcggccatgt
     3841 cgtcatcgtg aagctgttga atggccatct ctgcacgaat tttgtcgtct cgatacatgc
     3901 tctttcctcc taacacactg gaagatacat ttaccgaccg gtcgagtcaa gcaaaatgtt
     3961 aattaaacat tccactgctt taaatgcatc taccgttaag tgtgctaaac agagtggtgt
     4021 gagcaatgaa caggaaaccc tcggcgattt tgttcgccgg gtggctaatg aaaagggatt
     4081 gagtcaacgt gaggtcgcac gcagaagtgg tgggctcatc agtcatgtca ccgtgggcga
     4141 catcatcaat aaaaatcgag gtgacgttaa gactgaaaca ttaagagggc ttgcaaaggg
     4201 tttaggtgtg acggaagaag aagtcttcgc cgtggcgcgt ggcaagaaac cggaggacga
     4261 tcccgacttc ctcaaatctc gacttggctt gttgagcctt aagttcgaca agataccgat
     4321 agataagagg gtgaacgtcg aagccttaat cgagttgtta gatcgagagg tcgagcgtct
     4381 gacgaagaaa tgaacctcgc acttgttgcg ttgaaagcgt tcccacattg gaaccgacgc
     4441 atcattaccg agcgggacat ccagcgggtc tgcaaaaaga agggcgtgta catcgtcgag
     4501 ggcaaggtgg gagtgcccgg cctctacatt ccttacgcgg gcttcgcttt catcgtcatt
     4561 gaccccgacc tgccgccggg gatgcgtctc tgggtcttac tacacgaact ggcgcaccac
     4621 ctgctccacg tccccggcct gcagctcttc gacaagcggt acaagacgaa ggctgacttt
     4681 gaagcgaact tcatcgcggc cattggagcc gtaaccctcg agatggtcat gacgaagctc
     4741 cctggagaga ttcaagagga gtatggctac ccgaaggaac taaccgatct gcggaagtgg
     4801 atttacgata actacaggat ttgaattgat ctcgcgctga cttccgacgg cactgcttca
     4861 tcagactcaa acgctcgccc tgtttggctg ggcactaact aaaaccctct ctcacgctgg
     4921 cagagtatct ttatgagcga cgacctaagc atcttttcac gacatggcaa tgcagataca
     4981 agcagtcaac tttccagcct tccaaatgta gcacctgcgc tggagaggct gccgacgaat
     5041 cccaatctct tcccctgccc ggactgtact cacccctgct cgcggaatgc cgagacgtgc
     5101 ccgcagtgtg gccgattctt ccagcgtttt tataccggaa ggtttttgtc gaccggtctg
     5161 gctggtcaat gacgattgcg tgggg
//
LOCUS       DASWNS010000526         9573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_357257, whole genome shotgun sequence.
ACCESSION   DASWNS010000526 DASWNS010000000
VERSION     DASWNS010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357257"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..741
                     /gene="purN"
                     /locus_tag="VGB76_18060"
     CDS             139..741
                     /gene="purN"
                     /locus_tag="VGB76_18060"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY0100857.1"
                     /translation="MKLAILISGRGSNMLALAAAVARGRIHGARIALVLSDHADAAGL
                     RSAAELGLQTIAVERAGRTRAEHEREIISALQKHEVDLVCLAGFMRLLSPQFVEAYRG
                     RILNIHPSLLPAFPGLDAQRQAIEHGVKVSGCTVHFVDETLDGGAIVTQRVVPVYEDD
                     TPETLAARILPEEHLAYAEAVALVVSGNYEIDGRRVVRKI"
     gene            complement(796..1608)
                     /locus_tag="VGB76_18065"
     CDS             complement(796..1608)
                     /locus_tag="VGB76_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100858.1"
                     /translation="MSRRSERSVDLSETPASRRAKEKIVRLVICFAVALAFNVQPNFA
                     QQNPQNRVDAQTSETRQLPPVAVEAQPGSPLELSVRRMRWATPTPEVLDMYLAVKNIG
                     ALPVRAYATRTGKPEQGGCFIYNVTAPGKVIQPEQSDSRSRFVGVSSASAPEVIRVAV
                     DFVEFTDGTTWGEDRCETAELLAGERAGGRAAIELLREIFRQEGAEAVLQKIKNSALE
                     PEPPGDHSERWKSAFRGAIKTVVERVRRAYDEEGLKEIETMLNKPYDAAGAK"
     gene            complement(1605..2543)
                     /gene="proC"
                     /locus_tag="VGB76_18070"
     CDS             complement(1605..2543)
                     /gene="proC"
                     /locus_tag="VGB76_18070"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY0100859.1"
                     /translation="MTNSAATNKFNESANENSSGGRENPLGGVRLVFVGVGVMAESMI
                     AGLLKRRLVAPAQLSGSHPRAARRAELEAKYGIRVVEDNREAVARDPGASGEAEAGAQ
                     EACGAIIILAVKPQKLGGVLHELKDALHADDLVLSIVAGARIETMAGVLLHPAIVRAM
                     PNTPAQVGEGMTVWTATAQVDETRERQVAAMLGALGREMRVEEERQIDMATALSATGP
                     TYIFLVMESLVDAGVHMGFSREVARELVLQTMLGSVRFAMESHKHPAELRNMVTSPGG
                     TSAEAIYQMEKGGLRTVLSKAVYAAFQRAVALGQKK"
     gene            2655..3881
                     /gene="tyrS"
                     /locus_tag="VGB76_18075"
     CDS             2655..3881
                     /gene="tyrS"
                     /locus_tag="VGB76_18075"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY0100860.1"
                     /translation="MTIDEQLDYLRKGTVEIIGEDDLRKKLSLAGIRSAEGNKPLPPL
                     RIKLGADPTAPDIHLGHTVVLRKLRAFQELGHTVIFLIGDFTGLIGDPSGKNATRPQL
                     TREEINANAETYKTQIFKLLDPDKTEIRFNSEWMDALGSDGFIRLASHVTVKQILERD
                     DFEKRLSENRPLALHEVLYPLVMAYDSVALKADVELGGTDQKFNILMGRNLQREYGQD
                     PQVAIIMPLLEGTDGVQKMSKSLGNYIGINEPPQEMFGKVMSISDQLMWRYYELLTDL
                     RVEEINELRAAAHAGARNPRDLKVELAKRIITDFHSATDAQAAEDEFNRIFKRKEIPD
                     EIEERALTTGVWKLPRLLVETALAPSMGEARRLIEQGGVRVDGERRTEDEINLDAGQT
                     ILIQVGKRRFLRVRGV"
     gene            3968..4786
                     /locus_tag="VGB76_18080"
     CDS             3968..4786
                     /locus_tag="VGB76_18080"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0100861.1"
                     /translation="MSIAANEETLVIETPERVPLHFALASIGNRFLACAVDHGLQVFA
                     ILLVALVFLWTGYLTRAGDRLSEAPKWMLAAVIILVFVLWSGYFALFEWLWNGQTPGK
                     SLLKLRVIREDGRPVTLWEAAARNLLRLFDMMPFPFYSIGLVSVFASSRDQRVGDFVA
                     GTVVVREREAQAPTFAEVFNAPTSDVALRRSFKPVLFKGDTNALGEREIEVVETFLRR
                     RWELADATRQWMAWRVATPILYKLKPEFDRETFTYEGFLEELLHRYRAAHRFIN"
     gene            complement(4909..6246)
                     /locus_tag="VGB76_18085"
     CDS             complement(4909..6246)
                     /locus_tag="VGB76_18085"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0100862.1"
                     /translation="MFLSAFTRKALFVAAFALLTLSLAAPQTLHAQSLDRIERERALL
                     MLGNVKDQLKKHYYDPTIRGMDVEARFKTAEEKIKQATSLGQAFGIIAQVLIELDDSH
                     TKFYPPSRPERVEYGWEMQMIGDECYVVAVKPGSDAAAQGLKEGDLILAVERFKPSRK
                     EFWKMQYYYKTLSPRAGLNVVAQSPGGQPREMSLKAKVTPGKRVLNLNSDLEVNAILR
                     EMEDNAKLGRHRYYDDIGGVFVWKMPAFDLTDASIDEIMRKAQGRRAIVLDLRGNGGG
                     AESTLLRLLGHFFDHDVKMADIKTRKESKPVVAKTRGKNNLFEGKVVVLVDSESGSAS
                     EVFARVMQLEKRGTVVGDRSAGAVMRSNFREYEMGTERIVLWGANITNADVIMADGKS
                     LERVGVIPDELILPKATDMAARRDLVLARALELAGTKVDPARAGGLFPVEWKK"
     gene            6612..7793
                     /locus_tag="VGB76_18090"
     CDS             6612..7793
                     /locus_tag="VGB76_18090"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY0100863.1"
                     /translation="MAQDIFILGGKRTPMTQYVGALKDVKAIDLAATAARGALASTGV
                     AAEEIDHTIIGNALQTSGDAIYGARHVALKAGVPVDRPALTINRLCGSGIQSIVSGAH
                     MILLGEAQTALVGGMESMSQAPHVIYGARSGFPLGQGKLEDSLMVALLDTYCNTTMAG
                     TAENLARRYEISREDQDKYALRSQQEAKRAQDAGVFAEEITPVEVKTRKGVKVVDTDD
                     HLRPETTLEGLAKLPTAFSKDGFVTAGNASGIVDGAAALVIAGEEFVKARGVQPLGRV
                     VAWAYAGVEPDVMGIGPVPAVQKVLEKAGLKLADIDLVEVNEAFAGQYLAVEKELGLD
                     RARTNVNGGAIALGHPLGASGTRLVLTSLLELRRRGGRYALSTACIGGGQGIAIIVER
                     Q"
     gene            complement(8375..8542)
                     /locus_tag="VGB76_18095"
     CDS             complement(8375..8542)
                     /locus_tag="VGB76_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100864.1"
                     /translation="MAASLNFPLSPATPALAELLRAGQINSSSHPLNRRFRQTFAPNS
                     MACNLKFTPHL"
     gene            8755..>9573
                     /locus_tag="VGB76_18100"
     CDS             8755..>9573
                     /locus_tag="VGB76_18100"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0100865.1"
                     /translation="MSKLPLHRSACALMLLVLCLAFGVNPAAAQSTTDGAIGGLIKDP
                     NGAVVANAAITVRNEGTNKESTATSDDEGRFRVVQLQPGNYTVTIGVSGFAPFSQQHV
                     VVEVGRVTSLDIPLSISGSQEQVEVTAEAPVINTTQQDFSNNINQTSINELPINGRRA
                     SNFVLLTPGVVAEGGFGLNSFRGVSGLLNNSTLDGGDNNNAFYGEERGRTRISYVVSQ
                     AAIREFQVNTSNYSAEYGRAAGAVINTVTKSGTNDFHGDLFYYNRNNRFGATNPF"
BASE COUNT         1890 a         2951 c         2797 g         1935 t
ORIGIN      
        1 cgcacgcgcc gtggaattcg tctagcgagt ctcaagtctc aagtctgaaa acttaaagat
       61 cggacttgag actcaagagc ggcaaacttg cgacctgcaa acttgagacg cgggactttg
      121 agaactggaa actgagactt gaaactggcg attcttattt ctggtcgcgg ctcgaacatg
      181 ctcgcgctcg ccgccgccgt cgcgcgcggg cgcatacacg gagcacgcat cgcgctcgtc
      241 ctgagcgacc acgccgacgc cgccgggttg cggagcgcgg cggaactcgg actccagaca
      301 atcgccgtcg aacgcgccgg gcgcacgcgc gccgaacatg aacgcgagat catctcggcc
      361 ttacaaaaac atgaagttga tctcgtctgc ctcgcaggtt tcatgcgcct gctctcgccg
      421 cagttcgtgg aggcttatcg cgggcgcatc ctcaacatcc acccgagcct gctcccggcc
      481 ttccccggcc tcgacgcgca gcggcaggcc atcgaacacg gcgtcaaagt cagcggctgc
      541 accgtccact tcgttgacga gacactggac ggcggcgcga ttgtcacgca gcgcgtcgtc
      601 cccgtctatg aagacgacac gccggagacg ctcgccgcgc gcatcctccc cgaagaacat
      661 ctggcctacg cggaagccgt cgcgctcgtg gtgagcggaa actatgagat cgacggtcgc
      721 agagttgtga gaaaaattta gcgggtttcg cgtcgcgttt gcagagttgt cgggttcatc
      781 cggcgggcgg aattttcact tcgccccggc ggcgtcgtaa ggtttattga gcatcgtctc
      841 gatctccttt aacccttctt cgtcgtaagc gcgacggacg cgctcgacga ccgttttgat
      901 agccccgcga aatgccgact tccaacgctc cgaatgatct ccgggcggct ctggttcgag
      961 cgcggaattt ttgatctttt gtaagacggc ctccgcgcct tcctgtctga aaatttctcg
     1021 cagcagttcg atggcggcgc gtccgcctgc gcgttccccg gccagcaact cggcagtctc
     1081 gcaacgatcc tcgccccacg tcgtcccgtc cgtgaactcc acgaaatcga ccgcgacgcg
     1141 aatgacttcg ggcgcggagg cggaactgac gcccacaaac ctgctcctgc tatccgactg
     1201 ttcgggttga atgactttcc ccggcgcagt cacgttatag ataaaacagc cgccctgctc
     1261 aggcttcccg gtgcgtgtcg cataagcgcg aacggggagc gcgccgatat ttttaactgc
     1321 aagatacata tccagcacct cgggcgtcgg ggtcgcccaa cgcatccggc gcacgctcag
     1381 ttcgaggggc gagccgggct gcgcctcgac cgcgaccgga ggcagttgac gagtctcgga
     1441 cgtttgcgcg tcaactctgt tttgaggatt ttgctgcgcg aagtttggct gcacgttgaa
     1501 ggccagcgcg actgcgaagc agatgacgag tctcacaatt ttctccttcg cgcgccgcga
     1561 cgctggcgtc tcgctcaggt caacgctccg ctcacttctt ctgctcactt cttctgcccc
     1621 aatgcgacgg cgcgttggaa ggccgcatag acggcttttg agagcactgt gcgcaagccg
     1681 cctttttcca tctggtagat cgcttcggcg gaagtgccgc cgggcgaggt gaccatgttg
     1741 cggagttccg ccgggtgctt gtgggattcc atcgcaaagc gcaccgagcc gagcatcgtc
     1801 tgcaagacga gttcgcgcgc cacttcgcgc gagaagccca tgtggacgcc cgcgtccacg
     1861 agcgattcca tgacgaggaa aatgtatgtg ggaccggttg cgctcaaggc cgtcgccatg
     1921 tctatctgtc gttcttcttc gacgcgcatc tcacgcccga gcgcgccgag catcgccgcc
     1981 acctgtcgct cgcgcgtctc gtcaacctgc gcggtcgccg tccacacggt catcccttcg
     2041 cccacctgcg cgggcgtgtt cggcatcgcg cgcacgatgg ccgggtgcag caacacgccc
     2101 gccatcgtct cgatgcgcgc tccggcgacg atggagagca ctagatcatc cgcgtgcagg
     2161 gcgtctttca gttcgtgcag cacgccgccg agcttctgcg gtttgacggc gagaataatg
     2221 atcgcgccgc acgcttcctg cgcgcccgcc tccgcctcgc cgctcgcgcc cggatcgcgc
     2281 gccaccgcct cacgattatc ttcgacgacg cggatgccgt acttcgcttc gagttcggca
     2341 cgacgcgcgg cgcgcgggtg gctgccggag agttgcgcgg gcgcgacgag ccgccgtttc
     2401 aacagacccg cgatcatcga ctcggccatc acgcccacgc cgacgaacac gaggcgcaca
     2461 ccgccgagcg gattctcccg cccgccggat gaattctcat tagcactttc gttaaatttg
     2521 ttagtcgcgg ctgaattggt cataactaga aaagtagctc aaacacctga cagattcaaa
     2581 gctcttcaag cattcaacgc gcaaagcggc cttcgtctat ttacgattta cgattcacgt
     2641 tttacgattg agccatgacg attgacgaac aactcgatta cctgcgcaaa ggcacggtcg
     2701 agatcatcgg cgaagacgac ctgcgcaaga aattatcatt ggccgggatt cggagcgcag
     2761 aaggtaacaa gccccttccc ccgttacgta tcaagctcgg cgctgatcca accgcgccgg
     2821 acatccatct cggtcacacc gtcgttctcc gaaaattgcg cgcctttcag gaactcggcc
     2881 acaccgtcat ctttctcatc ggcgacttca ccggactcat cggcgaccct tcgggcaaga
     2941 acgccacgcg cccgcaactc acgcgcgaag agatcaacgc gaacgcagaa acctacaaga
     3001 cgcaaatctt caagctgctc gacccggaca aaaccgagat tcgcttcaat tccgagtgga
     3061 tggatgcgct tggctccgac ggcttcatcc gcctcgcctc gcacgtcacg gtcaagcaga
     3121 ttttggagcg tgacgatttc gagaagcgac tcagtgaaaa ccgcccgctc gctctgcacg
     3181 aagttctcta cccgctcgtg atggcctacg attcggtcgc gctcaaagcg gacgtggaac
     3241 tcggcggcac ggatcagaag ttcaacattt tgatggggcg caatctgcaa cgcgaatacg
     3301 ggcaggaccc tcaggtcgcc atcatcatgc cgctcttaga aggcacggac ggcgtgcaga
     3361 agatgtcgaa gtcgctcggc aactacatcg gcatcaacga accgccgcag gagatgttcg
     3421 gcaaagtcat gtccatctcc gaccaactca tgtggcgtta ctacgaactc ctgaccgacc
     3481 ttcgcgtcga ggagatcaac gaactccgcg ccgccgccca cgcgggcgcg cgcaacccgc
     3541 gcgatctgaa agtcgaactc gccaaacgca tcatcacaga ctttcattcc gcaacggacg
     3601 cgcaagccgc cgaagacgag ttcaaccgca tcttcaagcg caaggagatt cccgacgaga
     3661 tcgaagagcg cgcgctcacg accggcgtgt ggaaacttcc gcgcctgtta gtggaaaccg
     3721 cgcttgcgcc ttcgatgggc gaagcgcgca ggttgatcga gcagggcggc gtccgcgtgg
     3781 acggcgaacg acgcaccgaa gacgagatca atttggacgc gggacaaact atcctgattc
     3841 aagtcggcaa gcgtcgcttc ctgcgcgtgc gcggagtata aaaagcgaaa ctgcgcacag
     3901 ttgtaagtta gaataacagc gtctctaact tgtcacttgt cacccgtcac ctgtcactgt
     3961 tttaaccatg tctatcgccg ccaacgaaga gactttagtg atcgagacgc cggagcgcgt
     4021 gcctctgcat ttcgcgctgg cttcgattgg gaaccgtttt ctggcgtgcg cggtcgatca
     4081 cgggttgcag gtctttgcga tcttgctggt cgcgctcgtg tttttgtgga cgggttattt
     4141 gacgcgggcg ggagatcgtc tgtcggaagc gccgaagtgg atgctggcag ccgtgatcat
     4201 tttggtgttc gtgttgtggt cgggttattt cgcgctcttc gagtggctgt ggaatgggca
     4261 gacgccgggc aagagcttgt tgaaattgcg cgtcatacgc gaagacgggc gacccgtgac
     4321 gctctgggaa gcggcggcgc gaaacctcct gcgcctcttc gatatgatgc cgtttccgtt
     4381 ctactcaatc ggtctggtgt cggtctttgc gagcagccgg gatcagcgcg tgggcgactt
     4441 cgtggcgggc acggtcgtcg tgcgcgagcg cgaggcgcag gctccgacgt ttgccgaagt
     4501 cttcaacgcg ccgacgagcg acgtggcctt gcgtcgttcg ttcaagcccg tcctgttcaa
     4561 aggcgacacg aatgcgctcg gcgagcgcga gatcgaagtg gtcgaaacat ttctcaggcg
     4621 gcgttgggaa ctcgccgacg cgacgcgcca atggatggcc tggcgcgtgg cgacgccgat
     4681 tctctacaaa ctcaaacccg agttcgaccg cgagactttc acttacgaag gcttcctcga
     4741 agaacttctc caccgctatc gcgccgcgca ccgcttcata aattgagagc cgcgaaagga
     4801 cgacagcagc agtaacccct tcgcggctcc ggttggttat gcgccgcacg gccaggcgtg
     4861 tgcggcgttg cgcgcgctct cagataggct ggatacttcg cgcttctctt acttcttcca
     4921 ctcgacgggg aaaagccctc cggctctcgc ggggtcaact ttcgttccgg cgagttcgag
     4981 agcgcgcgcc agcacgaggt cgcgtctggc cgccatgtcc gtcgccttcg gtaagatcaa
     5041 ctcgtcggga atgacgccca cgcgctccag actcttgccg tcggccatga tgacatcggc
     5101 attggtgatg ttcgcccccc acagcacaat tcgctctgtg cccatctcgt actcccgaaa
     5161 gtttgaacgc atgaccgcgc ccgccgaacg gtcgccgacc accgtgcccc gcttctcaag
     5221 ctgcatcacg cgtgcgaaca cttccgacgc cgaccccgat tcgctgtcca ccagaaccac
     5281 gaccttgcct tcgaacaggt tgttcttgcc gcgcgtcttg gcgacgacgg gcttcgattc
     5341 tttgcgggtc ttgatgtcgg ccatcttcac gtcgtggtcg aagaaatggc cgagcaggcg
     5401 cagcagcgtg gactcggctc cgccgccgtt gccgcgcagg tcgagcacaa tggcgcggcg
     5461 accctgcgcc ttgcgcatga tctcgtcgat gctcgcatcc gtcaggtcga aggcgggcat
     5521 cttccagacg aacactccgc cgatgtcgtc gtagtaacga tgacgaccga gcttggcatt
     5581 gtcttccatc tcacgaagaa tcgcgttgac ctcaaggtcg ctgttgaggt tgagcacgcg
     5641 cttgcccggc gtcactttcg ccttgaggct catctcgcgc ggctggccgc cggggctttg
     5701 cgccaccaca ttgagtccgg cgcgcgggct gagggtcttg tagtaatact gcatcttcca
     5761 gaactctttg cgcgacggct tgaaacgctc gacggcgagg atgaggtcgc cctctttcaa
     5821 tccttgcgcc gccgcgtcgc tccccggttt gacggccacc acgtaacact cgtcgccgat
     5881 catctgcatc tcccagccgt actcgaccct ttcaggacgc gaaggcgggt agaacttcgt
     5941 gtgcgagtcg tcgagttcga tcaagacctg cgcgatgatg ccgaaggcct ggccgagaga
     6001 ggtggcctgc ttgatcttct cctctgcggt cttgaagcgc gcctccacgt ccatgccgcg
     6061 aatagtcggg tcgtaataat gttttttaag ttgatcttta acgttgccga gcatcaggag
     6121 agcgcgctca cgttcgatgc ggtctaagga ctgcgcgtgg agggtttggg gggcggcgag
     6181 cgagagggtc agcagggcaa aggccgcgac gaagagagcc ttgcgtgtga acgctgacag
     6241 aaacaacgcg ctctttttac tgcatgtcaa actattgtag gtcacgaggg tttactcctt
     6301 acggggaact tgatagaggc ttatttcgcg ggtaaaacgt ctgaggacgg tgactgcgag
     6361 gcggtcatct tgccacagac acactttgtt tggcaaacgt tttgcgggca cgccgggcga
     6421 cgattttgca cgcgcacgca ggcggcattt gcgccgtgcg ggggcacctt atggcctctc
     6481 tttttcatct taatagtaac gttcgagggc agtggatttc gcaagtttga cgccggtttg
     6541 cgcgtctgtt actttcaact gttttaccgg tcaactgaat tttcaaccat caagaggaag
     6601 tggaaaacat aatggctcaa gacattttta ttttaggcgg caagcgcacg ccgatgactc
     6661 agtacgttgg cgcgttgaag gatgtcaaag cgattgacct cgcggcgacg gcggcgcgcg
     6721 gtgcgctcgc ctcgaccggg gtggccgccg aagagatcga tcacaccatc atcggcaacg
     6781 ccttgcagac ttcgggcgat gccatctacg gcgcgcgcca cgtcgctttg aaggcgggcg
     6841 tgccggttga ccgtcccgcg ctcaccatca accgcctgtg cggcagcggc attcaatcaa
     6901 tcgtctccgg cgcgcacatg atcctgctcg gcgaagcgca aaccgcgctc gtcggcggca
     6961 tggaatccat gtcgcaagcc ccgcacgtca tctacggcgc gcgttccggc ttcccgctcg
     7021 ggcagggcaa actcgaagac agcctgatgg tcgcgctgct cgacacttac tgcaacacga
     7081 cgatggcggg cacggcggaa aatctcgcac gccgctacga aatctcacgc gaggatcaag
     7141 acaagtacgc gctgcgctcg cagcaggaag cgaaacgcgc gcaggatgcg ggcgtctttg
     7201 ccgaagagat cacgccggtc gaagtgaaaa cacgcaaggg cgtgaaggtg gttgacacgg
     7261 acgatcacct gcggcctgag acgacgctcg aagggttggc gaagttgccg acagcgttca
     7321 gcaaagacgg cttcgtgact gccggaaacg cttccggcat cgttgacggc gcggcggctc
     7381 tcgtcatcgc gggtgaagaa tttgtgaagg cgcgcggggt gcagccgctc ggtcgcgtcg
     7441 tcgcttgggc ttacgcgggc gtcgaaccgg acgtgatggg cattggtccc gtgcccgccg
     7501 tgcagaaggt gttggagaag gccgggctga aactcgcgga catcgatctg gtcgaagtca
     7561 acgaagcgtt cgcaggccag tatctcgccg tcgaaaaaga actcggactc gaccgcgccc
     7621 gcacgaacgt caacggcggc gcgattgcgc tcggccaccc gctcggcgca tcgggcacgc
     7681 gcctcgtgct cacgtcgctg cttgagttgc gccgacgcgg cggtcgttat gcgctctcca
     7741 ccgcctgcat cggcggcggt cagggcatcg ccatcatcgt cgaaagacag taagacagtg
     7801 acaagtgaca agtgacgagt tgaagacaag tttaaagaca ggttttcttc tgccttaact
     7861 tgtcactcgt cacttgtcac tgctttattg acttgtcact gcttctccat acgaacgccc
     7921 cggacaagct ctctcctcaa aaagcctgcc ggggcgtccg acgtttgcgc caacagtttt
     7981 cccccctctg gcgcaaattc caattccaca aactggcgga gttgacgaat ttcgaccgca
     8041 ctcgccactt cctgccgcgt cttattttca atccccgtgc cacacgcatt cgcccgtcag
     8101 cgcaagccgc aaagcgacgc taactgcggg agtttcaacg cgatggagcg ttcgccgcac
     8161 accgcgccct ttagacccgc gcgcgtgata cttgctacgc actttttcgc ccgccagcgg
     8221 aggatcgtga attaacggca gcaaacatgc acacttcaag acaagtgcga acgaagtagc
     8281 cggtgtgaac gaagtagcgg cgtgaaaaaa ttttgagagt tgccgggagc ggccttcgaa
     8341 gtagagcgcg gcgcgtagag cgggtcggag gcgtctagag atgcggcgtg aatttgagat
     8401 tacaggccat cgaatttggc gcgaaggtct gccggaatcg tctgttcagc gggtgcgacg
     8461 aactattgat ctgtccggcg cgtaataatt ctgcgagcgc gggggtggcg ggtgagagcg
     8521 ggaagtttag gcttgccgcc acgcccgatt tgtagtattt aacatcccgt ttatttggcc
     8581 gtaaggctcg cgtaaacagc ctgtcttatg atataaactt gctttcaagc aacgcagtct
     8641 tatcactacc tacatcaagg tctatcgcta acatttttgg acacggaagt tgccctctga
     8701 aggtttgccc tgtgtccgaa ctagccaaca atcgaaatta gaaaaggacg aaatatgtcg
     8761 aaactacccc tgcaccgctc agcctgcgcg ctgatgcttc tggtgctttg tctggccttc
     8821 ggagtcaacc cggcggcagc tcagtccacg acagacggag ctatcggcgg cttgatcaaa
     8881 gatccaaacg gcgcggtcgt agcgaacgca gccattacgg tgcgtaacga aggcactaac
     8941 aaggaaagca cggcgacttc ggacgacgaa ggccgtttcc gcgtcgtgca gttgcagccc
     9001 ggcaactaca cggtcactat cggtgtgtca ggcttcgctc ctttctcgca gcagcacgtc
     9061 gtcgtggaag tcgggcgcgt cacctcgctc gacatcccgc tctccatcag cggctcgcag
     9121 gaacaggtcg aagttacggc tgaagctccg gtcatcaaca ccacgcagca ggacttctcc
     9181 aacaatatca accagacctc gatcaacgag cttccgatca acggtcgccg cgcgtcgaac
     9241 ttcgtgttgt tgacgccggg cgtcgttgcc gagggcggtt tcggtctgaa cagttttcgc
     9301 ggcgtctccg gactcctgaa caacagcacg ctcgacggcg gcgacaacaa caacgccttc
     9361 tacggcgaag agcgcggacg cacgcgcatc agctacgttg tcagccaggc cgccatccgc
     9421 gagtttcagg tcaacacgtc gaactactcc gccgagtacg ggcgcgctgc cggtgccgtc
     9481 atcaacaccg tcaccaagag cggcacgaac gactttcacg gcgacctctt ctactacaat
     9541 cgcaacaacc gtttcggcgc gaccaacccg ttc
//
LOCUS       DASWNS010000527        16294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_279755, whole genome shotgun sequence.
ACCESSION   DASWNS010000527 DASWNS010000000
VERSION     DASWNS010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16294
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279755"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            529..2469
                     /locus_tag="VGB76_18105"
     CDS             529..2469
                     /locus_tag="VGB76_18105"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0100866.1"
                     /translation="MRKLLTIALALTLTLSLTFAIQPPSAAQEDEKLSATEQRLLEVA
                     ARNQGVAAGELQLLDSTTVNLPLTNRTVNTAKIVSTRDENILATSIDERGEEVDLALL
                     KQEEERAYVAKYGKLDPALYNRVQGLRSAERVKVAFWMNPVEDLDREEFRDGRTDVSR
                     EEVKAVLAKRSEQVRMATARATERFSATLRGAGFSIEERGGLSPVVYATLPASQLSAY
                     AARADVQRVYLAENRNEDYMNIAAPSIQANWVWFAGFTGADTGIAIIEDSRVDFDNNC
                     LVNNLGTRVPGDANVDQHATATAGMAASNNSTHRGIAYGAGIYSANGTDYADANMSAA
                     LDAAANNADILNNSWGPGCGGADGSMNVHARHADYIVRYVWDTVTAAAGNNGTCTDGE
                     FVGGIANGYNVIAVGNYDDKGTTSSTDNTMNSSSSYRNPTSTHGDREKPEVAAPGTNI
                     TSLLMTDSSDCPTGDVGSGTSYSAPMVAGVASLLMDSVPSLKYYPETVKAVIMAGAIN
                     NVEGSAALSDRDGVGGVNALSSTAILANKNYTWRYVTPSSFDANGYITIDMGWVEAYH
                     RVKVALVWDSNPSSDYTTDPLNADLDLNVVGPGVSVWSSRWDNSYETVDFTAPFAGNY
                     SIKIKNFRFNGANEYVAVAWNK"
     gene            complement(2814..4112)
                     /locus_tag="VGB76_18110"
     CDS             complement(2814..4112)
                     /locus_tag="VGB76_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100867.1"
                     /translation="MGPQYTRCTEAKDFSELNYVYVGLLLAVTVAGIQFALITAGLGG
                     WVAGAAFLEALRYILNWLLNGKLICLHRQSNPDCLCGGPAGVTVCAIGEIVDTENVGQ
                     DKNPIEDIDDDYSINLALFPFNIKDFSAKDFVDNKKIWRGTKRTYSDEFRAYLQSLSA
                     IATQPAQVQGDLLTRSQSVHGEVAEFGYLRTMVMLNNGNYLPWTEVVGRDPGKLLSGP
                     DEEDRWTDFMVKNVWLDPKKFSMPVMHCEFEGSRVHDMLKALEGFPFGGSFCKKNWFT
                     KLVCKIVAAVLAPLALLAVLLAWARNTSGSTDPAVVGGGTIGPKDRVIVRGSWVYDAG
                     HQGWNEIHAVRIVQRVDHVPSDPTAFKDFLHRWCDRLAETPTSDGFPQGPLGAYKPQD
                     VPAAELTLVSQRQPENQWEFHPLVDGCQPDDQPPSPPPIH"
     gene            complement(4126..5208)
                     /locus_tag="VGB76_18115"
     CDS             complement(4126..5208)
                     /locus_tag="VGB76_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100868.1"
                     /translation="MDCVLCRLAVRPAVLIALGIVLLMALWLGRAWLLAVLIAYLGLI
                     VLLGLIALALVGRRVLVKLCWKWQADPKGAGSVGHPKSHPVHIPASIYKRPDPLLYSQ
                     PWLVTRGLAVTWDNPDITVFEVQGSGLPPKMVPPHALLPNKDHLIRARIWNGAVDAPA
                     VNLLVRFHYLTFGIGAHRETIGEVLVPDLPVKGAAGLPRVAEMAWKTPALSGHYCVQV
                     ELVWTDDADQGNNVGQTNLDVKKLNSPNASFTFPLRNDGVLPARLRLTTDAYRLPPLD
                     SCDEDATGRRLRRIGRHLPEGNRLPSGWQVAISGVPDGAMEAGEERPITVKVTAVDGF
                     AGELDINVNAFDGQRLVGGVTLRVHS"
     gene            complement(5996..7528)
                     /locus_tag="VGB76_18120"
     CDS             complement(5996..7528)
                     /locus_tag="VGB76_18120"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0100869.1"
                     /translation="MFIHRRFFIACALACGAVFIVFGGSSQEKGMGRTSTMQAVSISL
                     EPCEVPGTREGVKEKARCGTYEVFENRVTKSGRRIKLKIVVFPATGRDKVADPLFYIT
                     GGPGSSATEDAPYIAGGFAKIRERRDLVFVDQRGTGGSNPLNCDFFNPSDPQSYFGVY
                     FPLADVRKCREQLEPKADLKLYTTDIAMDDLDEVRAALGYEQINISGASYGTRATQDY
                     LKRHAKHVRTVVLQGVSPTGQLMPRDFPQHTERALNGVLDECAADAACRAAFPNLRAE
                     VKAVLERLLRSPVAVEVKHPQTGAATRVALSRDLAGEAIRYMLYQSGAASRIPLFLHL
                     AAQGDFTPLAESAIFYRQRIVATGSNGMYLSVTCAEDLPWIKPGEGERNAAQTFLGDY
                     RLRQQRAACALWPRADIPKGYAEPTRADVPALILTGQWDPVTPPLYGDMAARYLPNSL
                     HLVVPHGGHGFGGLDGLDCIQNLTADFVNRGTTRGLDTSCVKNIRRRGFLLKLAEPKK
                     SP"
     gene            complement(7641..8066)
                     /locus_tag="VGB76_18125"
     CDS             complement(7641..8066)
                     /locus_tag="VGB76_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100870.1"
                     /translation="MSVWRRVALEKIPKLRRLIEAAPNIMALWIELQLKLADDALYKT
                     PSQEKTIAGIFDYASWCLNKSHNRDTQTAVVCAFYEHLPLMDEARKDLANRLSMEDFL
                     RLRERFKYLLTDEEHEEFTREFLERKAKPDSGMQRTRTR"
     gene            complement(8180..8716)
                     /locus_tag="VGB76_18130"
     CDS             complement(8180..8716)
                     /locus_tag="VGB76_18130"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY0100871.1"
                     /translation="MCSILTHPVVPLALSVFLPRDALSPALLLTGAVCSIIPDLDVIG
                     FRFGIRYSDMLGHRGLTHSILFAAALGALLSFTLFRVGQGDNWAVFLFLFLSTLSHTL
                     LDMLTNGGLGVALLAPFSNERYFFPWRPIEVSPIGIGSFFSEWGVRVICSELRWVWLP
                     SAIVSALGYVARRYWWSA"
     gene            complement(8878..9453)
                     /locus_tag="VGB76_18135"
     CDS             complement(8878..9453)
                     /locus_tag="VGB76_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100872.1"
                     /translation="MKISKIIVLTLTLFISSPAFSQQPGSSIHSVDFANFTYPWTTGL
                     SIRGGTKTFSLRDGKRPEVRDTSGHVEEIGVYLEQVSYGDVTGDGIEEAIIFMSILTG
                     VSAMPGQVYIYTLRGNHPTLLWHFSTGDRAEDGFRKVYADKGDLVVELNGPKRRGEGL
                     CCPERFTRTRYQWRGGRFRQKGRKEITPILE"
     gene            10084..13722
                     /locus_tag="VGB76_18140"
     CDS             10084..13722
                     /locus_tag="VGB76_18140"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY0100873.1"
                     /translation="MITLSAKADRRPATHPRPAAFRLLFTAIIAALLCAATLLTRSAT
                     AHDVTWVNGANGAIAGNSVEKKDGKFAAFDSNLNALRGRNNLSVAPFEPAPSAPPVPT
                     PAGEVVWVEDAVPTGSTVAGENEGWNWGSSNPAPFSGLSTHQSNIYSGRHQHYFYGAT
                     ATLALNAGDMLFTYVYLDPNNLPDEVMLQWHTDTFEHRAYWGANNIPWGTDGTNSRRY
                     MGALPPAGQWVRLEVPASSVGLEGATLNGMAFTLYGGRATWDYAGKSDGSPTPTPTPT
                     PEVVWVEDAVPAGGIAVADGGDSWNWISSNPSPFSGGLAHQSNLFSGEHQHFFYGATN
                     TLAVNAGEELFAYIYLDPANVPSEVMLQWHTGTFEHRAYWGANNIPWGTDGTNSRRYM
                     GVLPPAGQWVRLEVPASQVGLEGATLNGMAFTLYGGRATWDRAGKSSPTTPPTVGVSV
                     NANAVSTGATLNLYTDETILFDASASTSAYTHLWDFGDGSSGLKATTPKVAYGYRSTG
                     TRNVTYKACNNVGECSQLTFTITATARTFNSPGTTYTSLAALQTAIDADTGGSDFVLA
                     PGNYEGTLKLSKRTGLMTRIRCASGYGLPQVRTNDSAGMCVVTANENGQPALDTLPTT
                     GNSAPINNYLIEGFAFKFMAGANSSGSKVPVVRLGFNDSETFAQLPSNITLRHCHISI
                     PDDANAGQGIRPNARHVAVLDSTIKNIHYAHNGVGQDAQAIWGWHGMYGLWMDNCFLN
                     ADTEVAGAGSEGSTLTQRPEAITYRRTHFYKPETWTNSNSWWNKNIFELKAGRSVNVD
                     ACILETTDGDNNQGYPITLTAYRHTYPNIDTIEDVSFTRLKIKNTPGFASVCSDCNAL
                     QGNNGGEVRRVRFEDIVLDNQENTTRTGQGRFLLLQTSDTRALPIDRLSFNRITAPLN
                     YSGNVAAFIIFSIPTERRMTNFSFTNVVGNAVDAEIHSGVGKGTVALAANTSGGYTWA
                     GNVIAGITQNPPNDSSNEYPTSRNDIGFVNLNGGVGGDYRLSSSSLYQTAGTNNSRPG
                     ADIDAIDAATQGVISGVWQQSVSSANVNWADVANCTLTGTTIGQDGGSSIGHAESMQS
                     LGSGDGSFDFTVMQAGNYWGAGLNSGAKATSYAALEYAIVLRGTTNAEILESGTYRGE
                     VSVQANDVLRVAVESGKVKYYRIRGGSSLLVYMNETPSITYPLKANAIFLSTNSSIKD
                     GVVTGFSN"
     gene            14175..15125
                     /locus_tag="VGB76_18145"
     CDS             14175..15125
                     /locus_tag="VGB76_18145"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY0100874.1"
                     /translation="MSKDSKGFLKREDDAVQVAGTRIRTNYVVVAYLCLVHLIAASAL
                     FLPLRPGYLALAVFNYFFIGFSTTIGLHRLLSHRSFRCPKWAEYVLVTGAMLTGQGSP
                     LLWVANHRIHHQHSDEPGDVHSPRRGFWYSHILWIIDNNSTDSEAYRKYCKDLSADRY
                     YHWLVRYRLVPQLGAVLLCGFTLGWAAVPCVFFLPVVCWMHSTYSVNSFCHHDWFGSR
                     MFETREGSRNVWWVGLIALGEGWHNNHHACPRSVKHGMRWNQIDLSYLVIRLLASLGL
                     AWDLQQPAVTTQQQTKARPALPQQIRSTVSTNVGLTEPSS"
     gene            complement(15200..>16294)
                     /locus_tag="VGB76_18150"
     CDS             complement(15200..>16294)
                     /locus_tag="VGB76_18150"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0100875.1"
                     /translation="HTAFIFLWIALSEDIQARGELKMNYRRLGRTGLKVSELCLGTMT
                     FGSNFYNIGAVDQANANAIVARALEAGVNFFDTADVYSYGESETILGRAIKEAGVERR
                     SIVVATKVRGAMSEAAGSGTGDVNNVGLSRQHIISACEASLRRLGTDYIDLYQVHGWD
                     HLTPVDETLRALDDLVRQGKVLYLGCSNWSVRHLMKSLLVSELRGWERFVSLQAYYSL
                     VGRDLEHELLPLCLEEGLGVLPWSPLSGGFLSGKYRRDDPAPAGARRTGFTFPPIDEE
                     RGFDAVEALELLAKERDATPPQVALAWLLAQPGVASVIIGANKLSQLEDNLKSAGLNL
                     STEEAARLSATTAPRPLYPQWMIEVQSAGR"
BASE COUNT         3600 a         4759 c         4602 g         3333 t
ORIGIN      
        1 cacagcctcc tctccgcctc tcaccattct cctcacgccg cccgcgcctc ttccaaactc
       61 cttccccatc tttgcccgct cgtagcccga cgcctctgtc gctcgaaggc ttccaatgcg
      121 ctcgatattt atgccgggtg agcgggcgat aaatttctgc gtgggttgat cccttcgtct
      181 ctatctttcc gcgttagtta atgagcgtca ctcggaatag acggaacgga cgataaggtc
      241 tacgtctgac atctgaggat gccggtcatg gcaagcgtcg ggcaacgcat tgaaagtggc
      301 cgggcggacg ccaactgcga agccccgcgc gtctatatgc gcgacgcgcg gggcgcaagg
      361 attgacgcgt gcagacgccg ccgtcagttg ttcttttcag cctcattatc aggtgtcgga
      421 atttgcctcc ggcgacgcgt ctcgactgtt taccacgcgt cgcacggggg aactcgccaa
      481 agcggatgcc tgcctttatt aacctttact tgacaggaga aaaacctcat gagaaagctt
      541 ctcacgattg ctttggctct caccttaaca ctctcactaa ccttcgcaat ccagcctccc
      601 agcgcagcgc aggaggacga aaagctctca gccaccgaac agcgattgtt ggaagtggcc
      661 gcgagaaatc agggcgtcgc cgccggcgaa ttgcaattgc tcgacagcac gaccgtcaac
      721 ctcccgctca ccaatcgaac cgtcaacacg gccaagatcg tcagcacgag ggatgaaaac
      781 atcttagcga cctcgattga cgagcgcggt gaagaggttg atctcgcgct gctgaaacag
      841 gaagaggaac gcgcttacgt agccaagtat ggcaagctgg accccgcgct ctataaccgc
      901 gtacagggat tgcggagcgc ggagcgggtc aaagtggctt tctggatgaa ccccgtcgaa
      961 gacctcgacc gcgaggagtt tagagacgga cggacggatg tgagccggga ggaagttaag
     1021 gccgtgctcg ccaaacgcag cgagcaggta cgcatggcga ccgcccgcgc gaccgagaga
     1081 ttttccgcga cgctgcgcgg cgcaggcttc tccatcgaag agcgcggcgg cctcagcccg
     1141 gtcgtctacg cgacgcttcc cgccagccag ttgtcagcct acgccgcgcg cgcagacgtg
     1201 cagcgcgtct atctcgcgga gaaccgcaac gaggactaca tgaacatcgc cgcgccctcg
     1261 attcaggcaa actgggtctg gttcgcaggc ttcaccggag ccgacacggg catcgccatc
     1321 atcgaagaca gccgcgtcga tttcgataac aactgtctcg tcaataatct cggcacgcgc
     1381 gttcccggcg atgccaacgt agaccagcac gccaccgcca cggcgggcat ggccgccagc
     1441 aacaacagca cacatcgcgg catcgcctac ggcgcaggca tctacagcgc caacggaacc
     1501 gattacgcag acgccaacat gtccgccgcg ctcgatgccg ccgccaacaa cgcagacatt
     1561 ctcaacaaca gttggggtcc cggttgcggc ggcgccgacg ggagcatgaa cgtccacgcg
     1621 cgccacgccg actacatcgt gcgttatgtc tgggatacgg tgactgcggc ggcgggcaac
     1681 aacggcacct gcaccgacgg cgagtttgtc ggcggaatcg ccaacggcta caacgtcatc
     1741 gccgtcggca actacgacga caaaggcacc acctcgtcga ccgacaacac gatgaactca
     1801 tcttcgagct accgcaatcc gacctccact cacggcgacc gcgagaagcc ggaagtggcc
     1861 gcccccggaa ccaacatcac cagcctcctg atgaccgatt cgtctgactg cccgaccggc
     1921 gatgtcggtt ccggcaccag ttattccgcg ccgatggtgg caggcgtcgc gtcgctgctg
     1981 atggattccg tgccctccct caagtactac ccggagacgg tcaaggcagt catcatggcc
     2041 ggtgcgatca acaacgtcga aggcagcgcg gccttgagcg accgcgacgg cgtaggcggc
     2101 gtcaacgccc tctcctcaac cgccatcctc gccaacaaaa actacacctg gcgttacgtg
     2161 acgccctcat ccttcgatgc taacggctac atcacgattg acatgggatg ggtggaggct
     2221 taccaccgcg tcaaggtcgc gctcgtgtgg gattccaacc cgtcgagcga ctacacgacc
     2281 gacccgctca acgccgacct cgacctcaac gtcgtcggcc ccggcgtctc cgtatggtcg
     2341 tccagatggg acaacagcta tgaaaccgta gacttcaccg ctccgttcgc cggtaactac
     2401 agcatcaaga tcaagaactt cagatttaac ggcgcgaacg aatacgtcgc cgtcgcctgg
     2461 aataagtagt tgccgacgcc tacgcgtcac ctgaaaaata cccgctgcga aactaactaa
     2521 tcgcagcggg tatttgcatt agatacggtt gtaaaaatat ttcagccgcc gttaccagtg
     2581 cagggatgaa taattccagt tatcccactc tgtgagtaag aggctctgtg gcaaaagtcc
     2641 cgaaatcaaa agacatttgc ctcaactgaa aattctgatg caaatgtcta tgtcggggcg
     2701 tggtgctcgt cgcgcagccc tcgcgcccgt tctttgactg tctgagtgcg cgacataaag
     2761 cgttgcgtca ggcatttaat ttactcaggc tccaattatc tccgggggtg cgcttaatgg
     2821 atgggcggcg ggctgggtgg ttgatcgtcg ggctgacagc catcgactaa tggatgaaat
     2881 tcccactgat tttccggctg cctttgggac accagcgtca gttctgcggc gggaacatct
     2941 tggggcttgt aggctcctag tggtccctgg ggaaacccgt cgctggtcgg cgtttcggca
     3001 agacgatcac accaacggtg aaggaaatct ttgaacgccg tcggatcgct tggcacatgg
     3061 tctacacgtt gcacgatgcg caccgcgtgg atttcgttcc acccctgatg ccccgcgtca
     3121 tacacccacg agccgcggac gatcacgcga tccttgggac cgatggtccc tccgccaacc
     3181 actgccgggt ctgtcgagcc ggaggtgttt cgcgcccacg caagcagaac agccagcaag
     3241 gcgagcggag cgagcacggc agcgactatt ttgcagacga gcttggtgaa ccagttcttc
     3301 ttgcagaacg aaccgccaaa cggaaaacct tccaacgcct tgagcatgtc atgcactcgc
     3361 gaaccctcaa attcacaatg catgaccggc atcgagaact ttttcggatc cagccacaca
     3421 tttttgacca tgaaatccgt ccaccggtct tcctcgtcag gccccgatag gagcttccct
     3481 gggtcgcgcc cgactacctc ggtccagggc aggtaattgc cattgttcag catcaccatc
     3541 gtccgcaggt atccgaactc cgctacctct ccgtgtacac tctggcttcg cgtcagtaga
     3601 tcaccctgta cctgtgcagg ctgggttgca attgctgata gactttgcag gtatgctcgg
     3661 aattcgtcgg aataggtacg cttggtgcct ctccatattt ttttgttatc tacgaagtcc
     3721 ttggcggaaa aatcttttat gttgaaagga aaaagcgcga ggttgatgga gtaatcgtca
     3781 tctatgtcct cgataggatt tttatcctgt ccgacatttt cggtgtccac gatctcgccg
     3841 atggcgcaca ccgtgactcc cgccggcccg ccgcaaaggc aatccggatt cgactgacga
     3901 tgaaggcaga tcagtttgcc gttcaacagc cagttcaaga tgtagcgaag cgcctcaagg
     3961 aatgcggcac cggcaaccca accccccaat ccagccgtaa tcagggcaaa ctgtatccct
     4021 gcaaccgtaa cggcgagcaa taagcccaca tacacgtagt tcagttcgct gaaatccttg
     4081 gcttcggtac acctggtgta ttgcggaccc atcggccaac ctcctttagc tgtggacgcg
     4141 aagcgtgacg ccgccgacca gtcgctggcc gtcgaatgcg ttgacgttga tatcgagttc
     4201 gccggcgaag ccatcgaccg ccgtgacctt gacggtgatc ggtcgctcct cgcccgcctc
     4261 cattgctccg tcgggcaccc cgctgatcgc cacttgccag ccggacggta acctgttccc
     4321 ctctggcagg tggcgtccta ttcgcctcag acgccgaccg gtcgcatctt cgtcgcagga
     4381 atcgagcggg ggcaggcggt aggcgtcggt ggtcaggcgt aagcgcgcgg gcagaacgcc
     4441 atcgttgcgc agcgggaagg taaagctggc attgggggaa ttcagcttct tcacgtcgag
     4501 gtttgtctgg ccgacgttgt tgccctgatc ggcatcgtcg gtccatacca actccacctg
     4561 cacgcagtag tggcctgaca acgcgggggt cttccatgcc atttctgcca cacgcggcag
     4621 gccagcggcg cctttcaccg gcaagtcggg caccagcacc tcgccaatcg tctcccgatg
     4681 cgcaccgatt ccgaaggtaa gatagtgaaa gcggacgagg aggttgaccg ccggcgcatc
     4741 gaccgccccg ttccagatac gagcgcgtat cagatgatct ttattcggca aaagcgcatg
     4801 cggcggcacc atctttggcg gcagacccga cccctgcacc tcaaacacgg tgatgtctgg
     4861 gttatcccaa gtgaccgcca gtccgcgtgt gaccagccag ggttgactgt agaggagtgg
     4921 atcgggtcgc ttgtagatag acgctggaat gtgaactggg tggctcttcg gatgtccaac
     4981 ggagccggca cccttgggat cggcctgcca cttccagcac agctttacca gcacacgccg
     5041 accgaccagc gccagagcga taaggccgag taaaacaatc aacccgagat aggcgataag
     5101 caccgccagg agccaagctc tgccgagcca gagagccatc agcagcacta tccccagcgc
     5161 gatcagcacg gctgggcgaa cagcaagacg gcagagaacg cagtccatta tattctcctt
     5221 accttacttg gcgagtgatt aacaggcgca ctttcatctg gacaagtgct tcattaacga
     5281 accccgtcgc cggaaatgca gcacgcccca cactgaaatt atgagattta taaaacagtt
     5341 cggccagaat ccacgctcgc cctgacgctg atgcttgctc ataaaccgtt ctacgcgagg
     5401 aatctgttac aaggtagaca ttcaattctt cttaaaagaa tcggaataag tcatctctat
     5461 cgcctaaaac ccggcagcac ccgccccctg atggccgcag atgaaacggt gagcgaatta
     5521 actatcaggt tcggtaggat gagcgagtta gcagcagccg ggattacgcg atggcaggca
     5581 cggcggcggc ggtgacgagc tgacatgaga tgttcccgga gaacgggtga caaaccctga
     5641 cgggtgtgag ccgtctagac agtgaagcac gggcagacta tccgtgaagc ggggtatagt
     5701 caagaaaatt cttcagacaa ttcttcgggt tggttgatgg tcgagttgat aaacgtcagg
     5761 gcaagtctca ttgaacgccg aaacgatggg gttgaatgaa ggtcgccgga gagatgaatc
     5821 tttgaacgtg gcagaatcac tcttagggaa tatttcacct ggcttggcgc ttaacggaat
     5881 agtcaatccc tactgccatg cgatatttga tacatcactg agtcgggtgc tgcgctatgc
     5941 cctgagatca ataaacggat gcagccgtgc ctgaggatga gcggcgccgc gcgtgtcagg
     6001 gcgatttttt cggctccgcg agcttcagga gaaaacctct gcgacggatg tttttcacgc
     6061 aagaggtatc cagcccgcgc gtcgttccgc gattgacgaa gtcagcggtg agattctgga
     6121 tgcagtcgag gccgtcaagg ccgccgaagc cgtgcccgcc atgcgggacg acgagatgca
     6181 ggctgttggg aagatatctg gccgccatgt caccatagag gggcggcgtc accgggtccc
     6241 actggcctgt gagaatcagc gcgggcacgt cggcacgcgt cggctcggcg tagcctttgg
     6301 gaatatcggc gcgcggccag agtgcgcagg cagcgcgttg ctggcggagg cggtagtcgc
     6361 ctaagaatgt ctgtgccgcg ttgcgctcac cttcgccggg tttgatccac ggcaggtctt
     6421 cggcgcaggt cacggaaaga tacatgccgt tactgcctgt ggcgacgatc cgctggcggt
     6481 aaaaaattgc ggactcggcg agcggggtga agtctccttg cgcggccaag tgtaagaaga
     6541 ggggaatgcg gctcgcggcg cccgactggt agagcatata gcgaatagct tcccctgcga
     6601 gatcgcgcga gagtgcgacg cgcgtagcgg cgccggtctg cggatgcttc acctcaactg
     6661 cgacagggct gcgcagtaat ctctcaagca ccgccttcac ctcggcgcgc aggttgggga
     6721 aggccgcgcg gcaggccgcg tccgcggcac attcatccaa gaccccgttg agggcgcgct
     6781 cggtatgctg cgggaaatca cgcggcatca actggccggt cggagagacg ccctgcaaga
     6841 caactgtgcg gacgtgctta gcatggcgct tgaggtagtc ctgcgtggca cgcgtgccgt
     6901 aggaagcgcc gctgatattg atctgctcgt acccgagcgc ggcgcgcact tcgtccagat
     6961 cgtccatcgc aatatcagtc gtgtagagct tgaggtcggc tttcggctct aactgttcgc
     7021 ggcacttgcg cacgtccgcg agcgggaagt agacgccgaa gtagctttgc gggtctgacg
     7081 ggttgaagaa atcgcagttg agcggattag agcctcccgt gccgcgctgg tctacgaaga
     7141 caaggtcgcg gcgctcgcga atcttcgcaa atcctccggc gatgtagggg gcatcctccg
     7201 tggcagatga accggggccg cccgttatgt agaataaggg gtcggccact ttatcccggc
     7261 cagtagcagg gaagacgacg atcttgagct taatcctgcg cccgctcttc gtcacacggt
     7321 tctcaaagac ttcatacgtc ccgcagcgcg ccttctcctt cacaccctcc ctcgtgccgg
     7381 gcacttcgca aggctcaagc gagatggaaa cagcctgcat agtgctcgtg cgccccatgc
     7441 ccttctcttg actcgacccg ccgaacacaa tgaagaccgc gccacatgcg agcgcgcaag
     7501 cgatgaaaaa ccggcgatgt ataaacataa tttcctcagg agtggaaagc cgctaaccta
     7561 tcagagcgac ggctctcgct gctcgcagtc aactgacgca cggcaacagc ggcgtgcaca
     7621 gaacgtcaaa gataaaagac ctaccgggtg cgcgtacgct gcataccgct gtcaggcttc
     7681 gcttttcgtt cgagaaattc cctggtgaat tcttcgtgct cttcatccgt gagtaagtat
     7741 ttgaatcgct ctctcagcct taagaaatct tccatgctta aacggttggc taaatctttt
     7801 cgcgcctcat ccattagtgg gagatgctca tagaaggcgc acacgactgc cgtttgggtg
     7861 tcccggttat gcgacttatt caagcaccat gaagcgtagt cgaaaatacc tgcaatcgtc
     7921 ttttcctgag agggagtttt atacaacgca tcatctgcaa gttttagttg caactcaatc
     7981 cacagcgcca tgatgttagg ggcggcttca atcagccgcc tgagtttcgg gattttttcg
     8041 agtgcaactc ttctccaaac gctcatgcca ttcacctatc cgcgcgccat aagatagaag
     8101 cctaaccccc ggcatcaacc gcgcccacag gtaacgcgcg agagggcttc cgtcacatgg
     8161 catcgggtgc atgcggctgt caggccgacc accagtaccg ccgggcgaca tatcccagtg
     8221 cagacactat tgctgatggc agccagaccc acctcaactc gctacagatt acccttaccc
     8281 cccactcgga gaaaaagcta cctatgccaa tcggcgatac ctctatcggc ctccacggga
     8341 agaaatacct ctcgttactg aagggtgcta aaagggctac acccagccct ccgttcgtca
     8401 gcatatcaag gagcgtatgc gacagcgtcg ataagaataa gaagaggaac actgcccaat
     8461 tatcgccctg ccccacgcga aatagagtga agctcagcaa tgcgcccagc gcagcggcaa
     8521 acaggatcga gtgagtgagt ccccggtgcc cgagcatatc gctgtaccgg atgccgaacc
     8581 tgaaaccgat cacatccaag tccgggatga ttgagcagac ggcgccggtt aacagcaggg
     8641 cgggtgacaa ggcatcgcgt ggcaggaaga cagacagcgc gagcgggaca accgggtgag
     8701 ttaagattga acacataaga tcactacctg agcactaccg ggtcgggctg cctatcgccc
     8761 caattaagcc gcgccgaacg caggcatgca aacggcaacg cggggaagca tcttgagaag
     8821 catgttgtcg aggcgtcggg ttggatgagt tgttggattg cgttttacga ttccacttca
     8881 ctccagtatg ggcgttatct ccttccggcc tttctggcgg aatcttccac ctcgccattg
     8941 ataacgtgtt cgcgtaaacc gctccggaca acataagcct tcgcctctac gcttcgggcc
     9001 attcaactct acaacaagat cgcctttatc cgcatagacc ttgcggaatc catcctcggc
     9061 tcgatctccg gtagagaaat gccatagtaa cgtcgggtga tttccgcgta aggtatagat
     9121 atacacttgc cccggcatcg ccgagacgcc ggttaaaata ctcataaata tgatggcctc
     9181 ttcgatgccg tcgcctgtca catctccgta agatacttgc tccagataga cacctatttc
     9241 ctcaacatgc ccgctcgtat cacgaacctc cggacgcttg ccatctctga ggctaaaggt
     9301 cttggtgccg ccgcgaatgc tcagaccggt agtccacgga taggtgaaat ttgcgaagtc
     9361 aactgaatga atagatgaac caggctgctg agaaaaagca ggcgaagaga taaagagcgt
     9421 aagtgtcaag actatgattt tcgagatttt caaatgtacc ccgcagaaag cgttctaaag
     9481 cccgaatttg tatgttgact acagatggtt gatgcgcctc ttagcgcgca accgaatgct
     9541 cagggacgct tgagcacgag ggcaccggtg aagtttataa gcgccggagg cgaaagagcc
     9601 tgtgcgttgg cttagctccc ggtgtccatc ttgtgacaca taggccggac ggtatccgca
     9661 gcctctcttt gcagagaaga gcttgcgtcc acaaggtcgg ccaaacgcaa gtatacaact
     9721 accggggagt tctttcgacg actcttccga gcctcggcaa cgacatctcg aaactcaagt
     9781 ccgcgctcac ggatcatctc ttcgacagct cgataggaga gtcggtagcg gcagtaaccg
     9841 gcgtaggcag agcaggctga cagccggggc gtagtgccgc cgtttaaagg gcgtggagtt
     9901 acgtttcagg catagactcg gcgagtaaag ttaagagagc tagaggtggc tcttgtgcaa
     9961 acattctatt gggtgcaaga tacataaaac ggaagagata tttcctgtac tgtatgggag
    10021 ccgttttcaa ctacacgtcc aatttgcacc agagcctcac acatcaggca aggagttagc
    10081 tagatgatta cgttatccgc gaaagccgac cgccggccag ccactcaccc acgtcccgcc
    10141 gctttcagac tcctcttcac ggctattatc gcagccctcc tctgtgcggc gacccttttg
    10201 acgcggagtg ctaccgctca cgatgtgaca tgggtgaacg gtgcgaacgg cgctatcgcg
    10261 ggcaactctg tggagaagaa ggacggcaaa ttcgccgcct tcgacagcaa cttgaacgca
    10321 ctgcgcggca gaaataacct ctccgtcgcg ccgttcgagc ctgcgccgtc tgcgcctccg
    10381 gttcccacgc cggcgggcga ggtagtctgg gtggaggatg cggttcccac gggcagcacc
    10441 gtcgcaggcg agaatgaggg gtggaattgg ggaagcagca atccggctcc cttctccggc
    10501 ctctccacac accagtccaa catctacagc gggcggcatc agcactactt ctacggcgcg
    10561 acggccactt tagcccttaa cgccggggat atgctcttta cctatgtcta cttagacccg
    10621 aacaacttac ctgacgaagt gatgctgcaa tggcatacgg acacttttga gcatcgcgct
    10681 tactggggcg cgaacaacat tccatggggc actgacggca cgaacagtcg tcgctacatg
    10741 ggtgcgctgc ctccggctgg tcaatgggtc aggctggaag ttccggcgtc gtcggtcggg
    10801 ctggaaggcg cgacgctcaa cggcatggcc ttcaccctgt atggcggtcg cgccacttgg
    10861 gactacgcgg gcaaatcaga cggctcgccc acgccgacgc cgacgcccac tcccgaagtg
    10921 gtctgggttg aggatgccgt tcccgcaggc ggcattgctg tcgcagatgg gggcgatagc
    10981 tggaattgga tcagcagcaa cccgtcgcct ttttcgggcg ggctggcgca tcaatccaac
    11041 ctcttctccg gtgagcacca gcacttcttc tacggcgcga cgaacactct cgcggtcaac
    11101 gccggggagg agttattcgc ttatatctac ctcgacccgg cgaacgtgcc gagcgaggtg
    11161 atgctgcaat ggcacacggg cacgtttgag caccgcgctt actggggtgc gaacaacatt
    11221 ccgtggggca ctgacggcac gaacagtcgt cgctacatgg gcgtgctgcc tccggctggt
    11281 cagtgggtca ggctggaagt gcctgccagt caagtaggtc tggaaggcgc gacactcaac
    11341 ggtatggcct tcacgcttta tggtggtcgt gccacctggg atagagccgg taagtcgtct
    11401 cccactacgc ctccgaccgt cggtgtctct gtcaacgcta acgctgtgtc aacgggcgcg
    11461 actctcaacc tctacacgga tgagacaatc ctcttcgacg cttctgcttc cacatctgcg
    11521 tacacgcatc tctgggattt cggcgatggt tcgtcagggc tgaaagcaac gacgccgaaa
    11581 gtcgcttacg gctaccgctc gaccggcaca agaaacgtga cctacaaggc atgcaataat
    11641 gttggcgagt gcagccagtt gaccttcacg atcacagcca ccgctcgcac ctttaattcg
    11701 ccgggcacta cctacaccag tctcgcggca ttacaaaccg ctattgatgc cgacaccggc
    11761 ggcagcgact tcgtgctggc accgggcaac tatgaaggca cacttaaact ctccaaacgc
    11821 acagggctta tgacgcgcat tcgttgcgct tccggttacg gcctcccgca ggtacgtacc
    11881 aacgacagcg cagggatgtg cgttgtcacc gcaaacgaga acggccaacc cgcgctcgac
    11941 actttgccca caaccggaaa ctctgccccg atcaacaact atttaatcga aggcttcgcc
    12001 ttcaaattta tggcgggcgc gaacagcagc ggctccaaag tgccggttgt gcgactcggc
    12061 ttcaatgaca gcgagacttt tgcccaactg ccgtcaaata ttacgctccg ccattgccac
    12121 atttcgatcc ccgacgacgc caacgcgggg cagggcatca ggccgaacgc acgtcatgtg
    12181 gcggtgctgg attcgacgat taaaaacatt cattatgcgc ataatggggt tggacaggac
    12241 gcacaggcga tctggggctg gcacggcatg tacggcctct ggatggataa ctgtttctta
    12301 aatgcggaca cggaagtggc cggtgcgggc agcgaaggtt caaccctgac gcaacgcccg
    12361 gaggccatca cttaccggcg cacgcacttt tataaacccg aaacctggac aaacagcaac
    12421 tcctggtgga ataagaatat ctttgaactg aaggccgggc gttcggtcaa cgtcgatgcc
    12481 tgcatcttgg agacgacgga cggcgataat aatcaaggct acccgattac gctgacggct
    12541 taccgtcata cctacccgaa cattgacacg attgaagacg tgtcgttcac gaggctcaag
    12601 attaaaaaca cgcctggctt cgcatcggtg tgcagtgact gcaatgcttt acagggaaac
    12661 aacgggggcg aagtgcggcg ggtgaggttt gaagatattg tgctcgacaa tcaggagaat
    12721 acgacacgca ccgggcaggg gcgtttcctt ttactccaga catcggacac gagagctttg
    12781 ccaattgatc gcctctcgtt taatcgcatc acggcacccc tcaattacag tggcaacgtg
    12841 gccgcgttca taatattcag catccctacg gagcggagga tgacaaattt cagctttacc
    12901 aatgtggtag ggaatgcggt tgatgctgag attcattcgg gcgtcgggaa gggaactgtc
    12961 gcgctggcag caaatacatc cggcgggtat acgtgggctg gcaacgtgat cgcggggatc
    13021 acgcaaaacc cacccaacga tagcagcaat gaatacccga cgagtcgcaa cgatattgga
    13081 tttgtgaact tgaatggcgg ggtcggcgga gattatcgtt taagctcgtc gagcttgtat
    13141 cagactgcgg gcaccaacaa cagccgtccc ggcgcggata tcgacgctat tgatgcggcg
    13201 acacagggag tcataagcgg tgtgtggcag cagagcgtca gctcggctaa cgtcaactgg
    13261 gcggatgtgg cgaactgtac tctgaccggc acaactatcg gacaggatgg aggctcgtca
    13321 atcgggcatg cagagtccat gcagtcgctt ggttccggcg acggttcctt cgacttcacc
    13381 gtgatgcagg caggtaatta ttggggagcg ggtcttaatt caggcgcaaa ggcgaccagt
    13441 tacgcggcgc ttgagtatgc catcgtattg aggggaacga ccaatgcgga aatactggaa
    13501 tcagggacct atcgcgggga ggtgtcggtt caggctaatg acgttctaag ggtcgccgtc
    13561 gaatcaggca aggtaaagta ctatcgcatt cgaggcggct cttcattact cgtttatatg
    13621 aatgagacgc cgtcgattac ctatccgctg aaagccaatg cgatcttttt aagcaccaat
    13681 tccagtatta aggatggagt agtcacgggt tttagcaact gatgttccca cacaaacgtt
    13741 cgcgtccctg acgacgcagg ctccctgtat tgctgcctga gaaagaactc tcagtgacat
    13801 tccgcacggg gagataatct tcgccacacc gcacggcgag actgtagcgg ccttcgaaga
    13861 ttatctcccc tttttgtaac tactcctgcc agcttgagat catctgcctc tcgggatcaa
    13921 cctcgaagtg aatgtgactc gtacgcccgc catatctata cgtaagagac attctgtaaa
    13981 aacttaacgg aagcatcgcg ggcgtatcgc tctcgccggg ctgcgtgtgt gcccgcaatg
    14041 atcctgccgg acgttcaaat acgcgttagc tgatgaaggg ttaatgcgga agctatctct
    14101 gccggactcg atttatagag aagcagattt tggaatgtct ggtttgattc aaagagaagg
    14161 ttgattaaca agcaatgtcg aaagattcaa aagggttctt gaaaagggaa gacgacgcgg
    14221 ttcaagttgc gggaacgcga atacggacga actacgtggt tgtcgcctat ctctgtctcg
    14281 tccacttaat tgccgcctcg gcgctctttt tgcccctgcg tccgggctat ctcgcgctgg
    14341 cggttttcaa ctattttttc atcggattca gtaccaccat aggcttgcac aggctgctga
    14401 gtcacaggtc gttccgctgt ccgaaatggg cggagtacgt gctcgtgacg ggcgcgatgc
    14461 tgacggggca gggaagcccc ctgctgtggg tagccaatca ccgcatacac catcaacaca
    14521 gcgacgagcc gggagacgtg cattctccgc gcagggggtt ctggtacagc cacatcctct
    14581 ggataatcga taacaactcg acggactccg aagcctatcg caaatattgt aaggacttaa
    14641 gcgccgaccg ttactatcac tggctcgtgc gctacaggct cgtgccgcag ttgggggcgg
    14701 ttctgctgtg cgggtttacg ttggggtggg ctgccgtgcc gtgcgtcttc tttctgcccg
    14761 tcgtctgctg gatgcacagc acctattcgg tcaactcatt ctgccaccat gattggttcg
    14821 gctcacgcat gttcgagacg agagagggca gccgcaatgt gtggtgggtg ggtcttatcg
    14881 cgctcggcga aggctggcac aacaatcacc atgcctgccc gcgctccgta aagcacggaa
    14941 tgaggtggaa ccagatagac ctctcctact tggtgattcg actgcttgcg tcgctgggtc
    15001 tggcttggga cttacagcag cccgccgtaa cgacgcagca gcagacaaaa gctcgaccgg
    15061 cgttgccaca acagatacgc agcaccgtca gcaccaacgt cggacttaca gaaccctctt
    15121 cgtaaatgct tcgagtcttg agccgttgag cgggtcatgc gttaaggcgt tcatgcccgc
    15181 tcatgggagc gcgcgcaaat taacgcccgg cgctctgcac ctcgatcatc cattgcgggt
    15241 agagcggacg cggcgcggtc gtggccgata gtcgcgcggc ctcttccgtg gagaggttca
    15301 atccggcgga cttgagattg tcctcaagct gggagagttt gttcgcaccg atgatgaccg
    15361 acgcgacgcc gggttgtgcg agcagccacg cgagggcgac ctgcggcggc gtcgcgtcgc
    15421 gctctttcgc caacagttcc agtgcttcga cggcatcgaa tccgcgctct tcgtcaatcg
    15481 gcgggaaggt gaagcccgtg cgtcgtgcgc ctgcgggcgc gggatcgtcg cggcgatatt
    15541 ttcccgacag gaaaccgccg gagagcggcg accacgggag cacgccaagc ccttcttcaa
    15601 ggcagagcgg aagcagttcg tgttcgaggt cgcggccaac gagcgagtag taagcctgca
    15661 aagagacgaa ccgctcccac ccgcgcagtt ccgacacgag caggcttttc atcagatggc
    15721 gcacagacca attcgagcag ccgagataga gcaccttgcc ctgtcgcacg aggtcgtcga
    15781 gcgcacgcag cgtctcgtca acgggcgtca ggtgatccca accgtgtacc tgatacaggt
    15841 ctatgtagtc cgttcccagt cgtcgcaaac tcgcttcgca ggccgagatg atgtgctggc
    15901 gggaaagacc gacgttgtta acgtcgcccg tgccgctgcc tgcggcctcg ctcatcgcgc
    15961 cccgcacttt cgtcgccact acgattgagc ggcgttcgac tcccgcttct ttgatggcgc
    16021 gaccgagtat cgtttccgat tcgccgtaag agtaaacgtc tgccgtgtcg aagaagttga
    16081 cgcctgcctc caacgcacgc gcgactatcg cgttggcgtt cgcctgatca accgctccaa
    16141 tgttgtagaa gttcgagccg aaggtcatcg ttcccagaca caactctgaa accttcaatc
    16201 ccgttctccc aagccgccgg taattcatct tgagttctcc ccgcgcttga atgtcttcac
    16261 tcaacgctat ccataaaaat ataaaagccg tgtg
//
LOCUS       DASWNS010000528         4941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_97922, whole genome shotgun sequence.
ACCESSION   DASWNS010000528 DASWNS010000000
VERSION     DASWNS010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97922"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..909)
                     /locus_tag="VGB76_18155"
     CDS             complement(43..909)
                     /locus_tag="VGB76_18155"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0100876.1"
                     /translation="MNVSANHPVLVEGNCDSPVPGTTIVTPGTCGDYDGDGRIGTAED
                     TDGADRIFGTLKAALGPGTGAAAGTGANNNGRITIVRSGRFAESLLIGMTVPAFPDQG
                     TATPGNVTIEAAPGVEANLDAVLQGDPAGGNTARQAGVGILIFNGSNTTPTTVVTLRN
                     LVIRNFQEGIVTAGSTRINIDNCRIENNRDYGIHLTDFGQVVISNSQIQSNGFRIPVS
                     GPSSPGAGIRLEDAVQLRVVSSVISNNFGPGIINATGSQNTLVLFKVVSAFNNPDILG
                     FFTVAPDHNYSN"
     gene            1544..3028
                     /locus_tag="VGB76_18160"
     CDS             1544..3028
                     /locus_tag="VGB76_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0100877.1"
                     /translation="MRAFISAILTCVMLAATGGATTHTTFAQGRTRTTTNGAPASGGL
                     NSSPAQSVPGCKVAGLAMPGADLGAKINACDLALGAGGGKAEIHVDTGGTISTYVQLS
                     RGRTLWFHRGTYTLHQAYTGNPAQDIQTELGMIRYRDDVTIKGDGWETVIVEPPITNR
                     PTVFAPYISSVLTNGGYFHGVTRNVTIRDLQIRGQQRAASNGTYSTIEIGNSHNVLVE
                     NVFLNQTTGNGITAGGSSSVSSLNGCGGGRVETECASGVVMRGNRLFQVQNQGLNVVN
                     GRDVSILNNVIKDHNRISGSNGAGIDIETNTVTDTAKSIRIEGNMLDFSNSPQPFAGN
                     GINVQSSGQADYGPVEVRNNTLIGNALTPGSVGHMVFGIFVAAGTKRVTVAGNKIQRT
                     ANCGIIVYGAGHTIQDNELVSVSGGGNIALSLMGTNNSVIRRNKVRIDPSAQGGSDVI
                     AEVAGTGANNNVLEGNIADTPPKVVGAASKIASHRSWALSTLRP"
     gene            3236..4174
                     /locus_tag="VGB76_18165"
     CDS             3236..4174
                     /locus_tag="VGB76_18165"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0100878.1"
                     /translation="MSTRESTVGFEKAEVHTLGAATPDAPAAAPTPHLSIPSLPDEPL
                     VKIRPRSSWVALNLADLWDYRELLYFLTWRDIKVRYKQTALGAAWAILQPLFLMLIFT
                     LFFGRVAGLAPGGVPYPLFALTGLVPWTYFANAVTNSGNSLVGNANLITKVYFPRLLV
                     PMAAVAAGLVDFVLSFVLLVVLMFYYDVRLTWHVAMLPALVLLTTLAATGAGLLLSAL
                     NVKYRDIRFALPFMIQLWLFASSVIVPSSVLPERGRWLLSLNPMSGIIEGYRAALLGH
                     PFDWTALSISTAITLVLLAFAVLAFRRMEKQFADII"
     gene            4182..>4941
                     /locus_tag="VGB76_18170"
     CDS             4182..>4941
                     /locus_tag="VGB76_18170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0100879.1"
                     /translation="MATIIRVENLGKQYRLGGPQAAYSTLRETLVGAARAPLRWAQKG
                     KRPAAMVWAVRDVSFEVQPGEIVGIIGRNGAGKSTLLKMLSRITEPTTGAIDLYGRVG
                     SLLEVGTGFHPELSGRENIYLNGAILGMLRTEIARKFDEIVAFAEVEKFIDTPVKHYS
                     SGMYLRLAFAVAAHLEPEILIVDEVLAVGDARFQRKCLNKMQDVGQQGRTVLFISHSM
                     AAITRLCQRAILLNEGQVIADGPSDRVVSTYLHSG"
BASE COUNT          955 a         1444 c         1547 g          995 t
ORIGIN      
        1 ctttagtctt gcaagcgcaa gtgcttttcg gaactatgtc ggctagttcg aatagttgtg
       61 atccggcgcg acggtgaaga acccgagaat gtcggggttg ttaaaggccg agacgacctt
      121 gaagagcacg agggtgtttt gcgaccccgt agcgttgatg attccgggtc cgaagttgtt
      181 tgagataacg ctgctgacca cgcgcagttg cacggcgtct tcgagacgaa tgcccgcgcc
      241 ggggctagac ggtccggaga cgggaatgcg gaagccgttg ctctggatct gcgagttgga
      301 gatgacgacc tggccgaagt cggtcaggtg gataccgtag tcgcggttgt tctcaatgcg
      361 gcagttatcg atgttgatgc gcgtgctgcc cgccgtcacg atgccttcct gaaagttgcg
      421 gatgacgaga ttgcgcaagg tgacaacggt ggtcggggtc gtgttcgaac cgttgaagat
      481 caggatgccg acgcctgcct gacgcgcggt gttgccgccc gccgggtcgc cctggagcac
      541 cgcgtcgagg ttggcttcca cgccgggcgc ggcttcaatc gtcacgttgc cgggggtcgc
      601 cgtgccttga tcggggaagg ccggaacggt catgccgatg agcagagatt cggcgaagcg
      661 tccggaacgg acgatggtga tgcgaccgtt gttgttcgcg ccggttccgg cggcggcacc
      721 cgtgccgggg ccgagggcgg ctttgagcgt gccgaagatg cggtctgcgc cgtctgtgtc
      781 ttccgccgtg ccgatgcgtc cgtcgccgtc gtagtcgccg caggtgccgg gcgtgacgat
      841 ggtggtgccg gggacggggc tgtcgcagtt gccttcgacc agcaccggat gatttgccga
      901 tacgttcacg ctagtccaat tggtagttcc gaccaataac gcggcgaaca acagggaaca
      961 gagagtcatt ctgagaacag ggaagttaaa agccttagga gattctttca tcgttttcct
     1021 ctttccttca atggattcag ggcaagatac gcacgccgga cacccggccc actcgcaggc
     1081 gtccgcgtaa atttgtcggt actccgggca aaattgctgt gttcatgtca actacgttta
     1141 agtgagcatg atttcaatga acgaagcgtc ctgaatgttt gggtgcggca aaccgttgga
     1201 acgcagacct caatgctagg gcacgaaaat ctgaggatag aactcgaagc aggttgtgct
     1261 ctgatatacg cttacaaatg tagagtggat ttaacacaat cggtcgtgta ggtcaaaaga
     1321 aaaatttccc ccgtcttcgc cggaggcttc cggcaaagac gaaccggaca cgcccgcgcg
     1381 gcgaataatt cagagcgcgc gagcaaaccc tctgcgcgcg tcgaactctc taaacagtgc
     1441 cgccgcgact ttggcaagtg ggcgggcgag cgttagagtt gcgcgttaga aaaactaacg
     1501 gaagaaaaac ttcgtctgtg tcgcgcgtct ccgggagaaa tttatgagag cttttatatc
     1561 tgcgatctta acctgcgtaa tgctggccgc gaccggcggc gcgaccacgc acacaacctt
     1621 cgcgcagggg cgcacgagga cgactactaa cggcgcacct gccagcggcg ggcttaatag
     1681 ttcacccgct cagtctgtgc cgggctgcaa ggtcgcgggg cttgcgatgc cgggcgcgga
     1741 cttgggcgca aagatcaacg cgtgcgacct cgcgctcggc gcaggcggag gcaaagctga
     1801 gattcacgtg gatacgggcg ggacgatctc tacttacgtc caactcagtc gaggccggac
     1861 gctctggttt cacagaggca cttacacgct gcatcaagcc tacacgggca atcccgcgca
     1921 ggacatccag accgaactcg gcatgattcg ctatcgagac gacgtgacga tcaagggtga
     1981 cgggtgggag acggtgatcg tcgagccgcc catcacgaac cgccccacgg tcttcgcgcc
     2041 ttacatctct tccgtcttaa cgaacggcgg ctactttcac ggcgtcacgc gcaacgtcac
     2101 catccgcgac ttgcagatca gaggccaaca gagagccgct tccaacggca cctattcgac
     2161 catcgagatc ggcaactcgc acaacgtcct tgtcgagaac gtctttctca accagacgac
     2221 gggtaacggc atcacggcgg gcggttcgtc gagcgtctcc tctctgaacg gctgcggcgg
     2281 cgggcgcgtg gagacggaat gcgcgagcgg cgtggtgatg cgcggcaacc ggctgttcca
     2341 agtccagaat cagggcttga acgtcgtcaa cgggcgcgat gtgtcgatcc tgaacaacgt
     2401 catcaaagac cacaaccgga tcagcgggtc gaacggcgcg ggcatcgaca tcgaaacgaa
     2461 cacggtgacg gacacggcga agtccatcag gatcgaaggc aacatgctcg acttctccaa
     2521 ctcgccgcag cccttcgcag gcaacggcat caacgtgcaa tcttcagggc aggccgatta
     2581 tggccccgtc gaagttcgca acaatacgct catcggcaac gctttgacgc ccggctcggt
     2641 cgggcacatg gtcttcggca tcttcgtcgc ggcgggaacg aagcgggtca ccgtggcggg
     2701 caacaagatt cagcgcacgg ccaactgcgg catcattgtc tacggcgcgg ggcacacgat
     2761 tcaggataac gagttggtga gcgtcagcgg cggcggcaac atcgccctca gcctgatggg
     2821 cacgaacaat tcggtcatcc gccgcaacaa ggtgcggatc gatccgtcgg cgcaaggtgg
     2881 cagcgacgtg atcgcggagg tggccgggac gggcgcgaac aacaacgtgc tggagggaaa
     2941 catcgccgac acgccgccca aagttgtcgg cgcggcatcg aagatcgctt cgcatagatc
     3001 gtgggcgctc tcgacgctca ggccgtgagt cgttcgtttc gggggcgcgt ttcgtgggcg
     3061 gcaggcgagg gttgagtcgc ttgacgcgcg ggaagggcac ggttacactt gcccgtctta
     3121 ttaggctctg atcaaaactc cccgatgcaa ctatgccgca cgcgtgcgct tgtcgccgac
     3181 ttttttcggg cagctttgcc gcacgactta ctcttgaaga ttggatttga aggttttgag
     3241 cactcgcgaa agcacggtcg ggtttgagaa ggcagaagtt catacgctcg gcgcagcaac
     3301 acccgacgcg cccgccgcag ccccgacgcc ccacctgtca attccttccc tgccggatga
     3361 gccgctggtc aagatcagac cccgctcgtc gtgggttgcg ctcaacctcg cagacctctg
     3421 ggactaccgc gaactgctct actttctgac gtggcgcgac atcaaggtgc gctacaagca
     3481 gactgcgctc ggcgcggcgt gggcgatctt gcagccgctc tttttgatgc tcatcttcac
     3541 gctctttttc gggcgggtgg cgggactcgc ccccggcggc gtgccctatc cgctctttgc
     3601 tctcaccggg ctggttccgt ggacctactt cgccaacgcc gtgaccaata gcggcaacag
     3661 cctcgtcggc aacgccaatc tcatcaccaa agtctatttc ccgcgcctgc tggtgccgat
     3721 ggcggcggtg gcggcggggc tggtcgattt cgttctctcc ttcgtgctgc tcgtcgtgct
     3781 gatgttttac tacgacgtgc gtttgacgtg gcacgtcgcc atgctgcccg cgctcgttct
     3841 cttaacgacg ctcgcggcga cgggagccgg actgttgctc tcggctctga atgtcaagta
     3901 tcgagacatt cgtttcgcgc tgcctttcat gattcaactg tggctgttcg cttcctccgt
     3961 catcgtgcct tcgagcgtgc tgccggagcg cgggcgttgg ctgctctcgc tcaatccgat
     4021 gtcgggcatc atcgaaggct atcgcgccgc gctgctcggg catcccttcg attggactgc
     4081 gctttccatc tcaactgcaa tcacgctggt cttgctcgcc tttgcagtcc ttgccttccg
     4141 gcgaatggag aaacagttcg ccgacatcat ttagacccgc gatggcaaca atcatcagag
     4201 ttgagaatct gggtaagcaa tatcgcctcg gcggtcctca ggcagcttat tcgactttac
     4261 gcgaaacgct tgtcggcgcg gcgcgtgcgc ccctgcgttg ggcgcaaaag gggaagcgtc
     4321 cggcggcgat ggtctgggct gtgcgcgacg tgagtttcga agtgcagccc ggagagatcg
     4381 tcggcatcat cggcagaaac ggcgcgggca agtccacgct cttgaagatg ctctcgcgca
     4441 tcaccgaacc gacgacgggc gcaatcgacc tctacgggcg cgtcggctcc ctgctcgaag
     4501 tcgggacggg gtttcacccg gaactgagcg ggcgcgagaa catctacctg aacggcgcga
     4561 tcctcggcat gctgcgcacg gagatcgcgc gcaagttcga cgagatcgtg gccttcgccg
     4621 aagtggaaaa gtttatcgac acgcccgtca agcattattc gagcgggatg tacctgcggc
     4681 tcgctttcgc ggtggcggcg catctggaac cggagattct gatcgtcgat gaagtgctgg
     4741 ccgtgggcga cgcgcgcttc caacgcaagt gcctgaacaa gatgcaggac gtgggacaac
     4801 aggggcgcac cgtcctcttc atctcgcata gcatggcggc catcacgcgc ctgtgccagc
     4861 gcgccattct gctcaacgaa ggacaggtca tcgccgatgg tccatcggat cgagtggtga
     4921 gcacttacct gcattcgggt t
//
LOCUS       DASWNS010000529         3537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_330689, whole genome shotgun sequence.
ACCESSION   DASWNS010000529 DASWNS010000000
VERSION     DASWNS010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330689"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VGB76_18175"
     CDS             <1..1124
                     /locus_tag="VGB76_18175"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEY0100880.1"
                     /translation="LYDSLHTYTRWKLSEKYGKQLVPENQPIPAHLLGNMWSQTWSNI
                     YPLLKPEKGDAGFDLTKILVERKTDAKQMVRYGEGFFTSLGLEPLPQTFWERSLFVKP
                     QDREVVCHASAWDLDAQKDVRLKMCIKITAEDFSVVHHELGHNYYQMAYAPQPTLYQN
                     SANDGFHEAIGDAVALSVTPEYLKKINLISKVPDESADIGLLLRLALDKVAFLPFGYL
                     VDQWRWKVFSGEVTPENYNQAWWELRTKYQGIAPPAPRSEADFDAGAKYHVPANTPYA
                     RYFLAAILQFQFHRALCREAGFTGPLNRCSIYGNKQAGAKLKQMLAMGQSRPWPEALK
                     ALTGEEKMDATAIIDYFAPLKRWLDDQNARHARQTASVR"
     gene            complement(1254..2171)
                     /locus_tag="VGB76_18180"
     CDS             complement(1254..2171)
                     /locus_tag="VGB76_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100881.1"
                     /translation="MSRIVFVLLLMMAGQSVCLSQASGNIGYSQSGGNARAEQNERNK
                     RVLTKEDAPPSATSMFVEASVLMNVKANEHVAVFGLAQECVTVAECNQKMDATVSQFS
                     GDLKRLGIASNALFVDFAAQNKIYGFQLEGNVAKEKLVGFELKKNISVHYEDKLLLDK
                     LLVAAAQAKIFDLIKVDYIVRDTGSIHNRLADEAARIIKQKMARHEKLLAIKLQPPAQ
                     IFAERYSAYFPTEMYDSYTAYESEDVSRRSYDEDKYIIQRARKSRTFFFNPLNADGFD
                     AVINPIVIEPVVQFTLYLRVKYEVEQNNR"
     gene            complement(2224..2952)
                     /locus_tag="VGB76_18185"
     CDS             complement(2224..2952)
                     /locus_tag="VGB76_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100882.1"
                     /translation="MSTRKQGAAQETSRPAAHRGETFAGAAGCRRKFLKYFPQGFNDA
                     TYLSWERDYKWESHERWEEALGQAEFRRLLREGAFAEIAARAVRVEQRSRHSMIFSFE
                     KMALRDAIKPEEGARAFAEGLYEFLHGAGGAKRKFERWVETVDALPRRQTRVLTWPLV
                     TVFSFIAQPDRHIFLKPNVTRTAARAYGFDFPYKSRPNWDTYADYLTFAETLRRDLSD
                     LKPRDMIDVQSFIWVQGSDEYPDR"
     gene            complement(2995..>3537)
                     /gene="fusA"
                     /locus_tag="VGB76_18190"
     CDS             complement(2995..>3537)
                     /gene="fusA"
                     /locus_tag="VGB76_18190"
                     /inference="COORDINATES: protein motif:HMM:NF012882.4"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0100883.1"
                     /translation="QFVDKIVGGAIPQNFRPAIEKGIVEAAQSGSIAGYPLVDFRVTL
                     VDGSYHNVDSDEHSFRAAGRKAFRAAIDKVKPSLLEPIMDVEVVAPVEASGDIMGDLN
                     SRRGRVQGMEMRGKNQVIKAQVPMAEMLDYQSKLNSITAARGSYHMEFSHYDPVPGQL
                     THKIVEQARAEGRIRGEEED"
BASE COUNT          707 a         1094 c          999 g          737 t
ORIGIN      
        1 cgctctacga ttcgctccac acctacacgc gctggaagct ctcggaaaaa tacggcaagc
       61 aactcgtccc cgaaaaccaa ccgatccccg cgcacctcct gggcaacatg tggagccaga
      121 cgtggagcaa catctacccg ctcctcaagc ctgagaaggg cgacgccggt ttcgatctca
      181 cgaagatact cgtcgagcgg aagacggacg cgaagcagat ggtgcgctac ggcgaaggct
      241 tcttcacctc gctcggactc gaaccgctgc cgcagacctt ctgggagcgt tcgctcttcg
      301 tcaagccgca ggacagagag gtggtctgcc acgcgagcgc gtgggacctt gacgcgcaga
      361 aggacgtgcg cctcaagatg tgcatcaaga tcactgcgga agatttctcc gtcgtccatc
      421 acgaactcgg ccacaactat taccagatgg cctacgcgcc gcagccgact ctgtatcaga
      481 acagcgcgaa cgacggcttt cacgaagcca tcggcgacgc ggtggctctc tccgtcacgc
      541 cggaatattt gaagaagatc aacttgatca gcaaagtgcc ggacgagagc gcggacatcg
      601 gtttgctgct ccggctcgcg ctcgacaagg tggcgttttt gccgttcggt tatttagttg
      661 accagtggcg ttggaaagtg ttttcgggcg aggtcacgcc ggagaactac aatcaggcat
      721 ggtgggagtt gcgcacgaag tatcagggca tcgcgccgcc cgcaccgcgc agcgaagccg
      781 acttcgacgc gggcgcgaaa tatcacgtcc ccgcaaacac gccctacgcg cgctactttc
      841 tcgccgccat tctacagttt caattccatc gcgccctctg ccgcgaagcg ggcttcacgg
      901 ggccgctcaa ccgctgctcg atctacggca acaagcaggc gggcgcgaag ttgaagcaga
      961 tgcttgcgat gggacagagc cgcccctggc cggaagcgct caaagctctg acgggcgagg
     1021 agaagatgga cgccaccgcc atcatcgact acttcgcccc gctcaaacgc tggctcgacg
     1081 accagaacgc acgccacgcg cgccagacgg cctccgtcag gtgagagcgt tagttcaaac
     1141 ttaaagagca accgctcgac gcttatctct aacctgtgcg ccttcgcgtg aaaattaaat
     1201 tcacgcgaag gcgcacagtc acaaagagac gcgagatgtt ttgtcgcgcg tcgctacctg
     1261 ttgttctgct ccacctcata cttgactctc agatagagcg tgaactgcac caccggctcg
     1321 atcactatcg ggttgatcac cgcgtcgaag ccgtcggcat ttaaggggtt gaagaagaac
     1381 gtcctgctct tgcgcgcccg ctgaatgatg tatttgtctt cgtcataact cctccggctg
     1441 acatcctccg actcataggc cgtgtaggag tcgtacatct ctgtggggaa gtaggcgctg
     1501 tatcgctcgg cgaagatttg tgcggggggc tgaagtttga tggcgagcag tttttcatgc
     1561 cgcgccatct tctgcttgat gatccgcgcg gcttcgtccg ccagacggtt gtggatgctg
     1621 cccgtgtcac gcacgatgta gtcaactttg atgaggtcga agattttcgc ctgcgccgcc
     1681 gcgacgagca acttatcgag caggagttta tcctcataat gcacggagat attcttcttt
     1741 aactcaaagc cgaccaactt ctccttcgcc acgttgcctt ccaactgaaa gccgtaaatc
     1801 ttattctgcg ccgcgaagtc aacgaagagc gcattgctcg caatgcccag acgcttcaag
     1861 tcgccggaga actggctgac cgtggcatcc atcttctgat tgcactcggc gacggtgacg
     1921 cactcctgtg cgagtccgaa gactgcgacg tgctcattgg ccttgacgtt catcagcacg
     1981 ctcgcctcga cgaacatact cgtagcggag gggggcgcat cctctttcgt cagcacacgc
     2041 ttgtttcgtt cgttctgttc ggctctggcg ttaccaccgg attgcgagta gccgatattg
     2101 ccgctggcct gcgagaggca gacgctctgc ccggccatca ttaacagcaa cacgaataca
     2161 attctgctca tccttatctc cttcaggctt gaggtgaaac gcgcgactgc acccgtcgta
     2221 aattcatctg tccgggtact cgtccgagcc ttgcacccag atgaatgatt gaacgtcgat
     2281 catgtcacga ggtttcagat cgctcaggtc gcgccggagc gtctcggcga atgtgaggta
     2341 gtcggcgtag gtgtcccagt tggggcgcga cttgtacggg aagtcgaaac cgtaagcgcg
     2401 tgcggctgtg cgtgtgacat tcggtttgag gaagatgtgc cggtcgggct gcgcgatgaa
     2461 actgaagacc gtgacgagcg gccacgtcag cacgcgcgtc tgcctgcgcg gcaacgcatc
     2521 caccgtctcc acccaacgct caaacttacg tttcgcgccg cccgcgccgt gcagaaattc
     2581 gtaaagccct tcggcaaagg cacgcgcgcc ctcctccggc ttgatcgcat cacgcagagc
     2641 catcttctcg aacgagaaga tcatgctgtg gcggctgcgc tgctcgacgc gcaccgcccg
     2701 cgccgcgatc tccgcaaacg cgccctcgcg cagcagacgg cgaaactccg cctgccccaa
     2761 agcctcctcc caacgctcgt gcgactccca cttgtagtcg cgctcccaac tcaagtaggt
     2821 cgcgtcgtta aaaccctgcg ggaaatattt gaggaactta cgacggcatc cggcggcgcc
     2881 cgcgaaagtc tctcccctat gtgccgcagg gcgcgacgtt tcctgcgccg cgccctgttt
     2941 acgcgtggac attctgcttc aaactcctcg ctcgctggtt ggtgacaacc ccgcttaatc
     3001 ttcctcttcg cctctgatcc tgccttcggc gcgggcttgt tccacgatct tgtgtgtgag
     3061 ttggccgggg acgggatcgt agtgggagaa ctccatgtgg taggagccgc gtgcggcggt
     3121 gatggagttg agcttggatt gatagtcgag catctcggcc atcgggactt gcgctttgat
     3181 gacctgattc ttgccgcgca tctccatgcc ttgcacgcgc ccgcgccgcg agttgaggtc
     3241 gcccatgatg tcgccgctcg cttccacggg cgcgacgact tccacgtcca tgatcggctc
     3301 aagcagagag ggcttcactt tatcaatcgc cgcgcgaaac gccttgcgcc cggcggcgcg
     3361 gaaactgtgc tcgtccgagt ctacgttgtg ataagaaccg tcaacgagcg tgactctgaa
     3421 atcgacgagc gggtatccgg caatcgagcc ggactgcgcg gcttcgacaa ttcctttttc
     3481 aatcgccggg cgaaagttct gcggaatcgc gccgccgaca atcttatcga caaattg
//
LOCUS       DASWNS010000530         3965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_436441, whole genome shotgun sequence.
ACCESSION   DASWNS010000530 DASWNS010000000
VERSION     DASWNS010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436441"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VGB76_18195"
     CDS             <1..932
                     /locus_tag="VGB76_18195"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY0100884.1"
                     /translation="MRIFAGDKVKALMQRLGMEKGVAIESGMVSKRIEAAQKSVEARN
                     FESRKHLLQYDDVMNKQRETIYGLRRQLMEVPDQREYLLGDTGVAHDLLLDMSQQYIP
                     PTAAPDEWDLENYRIQLLTVFDFDAEAEGIDFESSTAEEIQATIWEKLKAHYAAKEAQ
                     IGSEAMRTYERIIMLNIIDAQWKDHLLSLDHLKQGIGLVGYGQKDPLVEYKKQSFDLF
                     QAMLDRIDTSTIRSLFNLQVVAEQAPEQLQQQRRRRTTPSSLTFTGPNQTAAAAGTED
                     GKIRTVTRDQPKVGRNDACPCGSGKKYKKCCGA"
     gene            999..2117
                     /locus_tag="VGB76_18200"
     CDS             999..2117
                     /locus_tag="VGB76_18200"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0100885.1"
                     /translation="MRFTRINLAPSFVSLLNACVCLLLFGAGGTPHAQLHAQATPPTA
                     SSSGVNLPTDTARGIELYKAANYREAIEVLRGAVKERREDGEAWLHLGIALSRADENK
                     EARKAFEKAIKLKPDAPQAHIGMAYLLLESGKPKEAERAATHALTLNARNAEAHYALG
                     LIHLRQNAPAKALAEADATLQLNPTFAGALLLKVEAAKEAYAAAYSEHFEKLKKLKTV
                     PEVSTEERAAVNNLLKEASAALDRYLALFPGAPNATRLREEAETLRFYSQPLNKSGEP
                     FAYPVNALTTKAQITSKPEPLYTEQARHGGISGTVRLRMVLAFDGKVRHILVVQGLGG
                     GLSEMAVEAARKIKFTPATRDGRPVSQFVTIEYNFHIR"
     gene            2209..3420
                     /locus_tag="VGB76_18205"
     CDS             2209..3420
                     /locus_tag="VGB76_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100886.1"
                     /translation="MSANISSTNPVVQAIAAGTAPQTARLMAARGMLPLAAEELLEVL
                     LVLRSDENQEIVAAANETLDEQPAETLLSVAQAAETSPVVLSYLATRDGAPREVSEAV
                     TLNTATPDEAIARMASATSDGSLLELISVNQQRLIRAPEIIEALLTNPARTNDAERRA
                     RETKREFFEKERGAQQIADEMRARGMSAAAEFVEAAESIGTGSGLTYDDAWLLAQHIE
                     VSDADIDDSWLPLERMEEFFTESEEQRAANLERIIAETTAEAGEVAPERISLIRRVML
                     MSVKDRVKLAMKGDREARGILIRDSNKVVATGVINNPRITDKEVEGISAMRTVSDEVL
                     RLIAQNRAWARNYPVIHNLARNPRTPIATAINVLLRLHTKDLQHISQNRNVSDAVRRQ
                     AQRLAQTRTGH"
     gene            complement(3576..>3965)
                     /locus_tag="VGB76_18210"
     CDS             complement(3576..>3965)
                     /locus_tag="VGB76_18210"
                     /inference="COORDINATES: protein motif:HMM:NF021503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HEY0100887.1"
                     /translation="PDLDIQSRQYTRWGVFRFIWWPYKVMFRHRSRWSHGIIFGTLIR
                     VVYFALMLSLIAVSAIYLRALLVAAAPPDFGELVRAWQAIEAGARIEGTGRHVALAVL
                     AGLWWGAASHTLTDVAWSVLRKGSEIF"
BASE COUNT          891 a         1255 c         1161 g          658 t
ORIGIN      
        1 tgatgcgcat cttcgcgggc gataaagtta aagccctgat gcagcgtctg gggatggaga
       61 agggcgtcgc gattgaatcc gggatggtgt cgaagcgcat cgaggcggcg cagaagtctg
      121 tcgaggcgcg caacttcgag agcagaaagc acctcctgca atacgacgac gtgatgaaca
      181 agcagcgcga gacgatctac ggcttgcgcc gccagttgat ggaagtgcca gaccagcgcg
      241 agtatctctt aggcgacacg ggcgtcgcgc acgatctgct gctcgacatg tcgcagcagt
      301 acatcccgcc gaccgccgcg cccgacgagt gggacttgga gaactatcgc atccagttgc
      361 tcaccgtctt cgacttcgac gccgaggccg aagggatcga tttcgaatcc tccaccgccg
      421 aagagattca ggcgaccatc tgggagaaac tcaaagccca ctacgccgcc aaagaagcgc
      481 agatcggcag cgaagcgatg cgcacttacg agcgcatcat catgctcaac atcatcgacg
      541 cgcagtggaa agatcacctg ctctcgctcg accatctgaa gcagggcatc ggactcgtcg
      601 gctacgggca gaaagacccg ctcgtcgaat acaagaagca gagcttcgat ctctttcagg
      661 cgatgctcga ccgcatcgac acgagcacga tccgctcgct cttcaacttg caggtcgtcg
      721 ccgaacaagc tcccgaacag ttgcagcagc aacgacgacg gcgcaccacc ccgtcttcgc
      781 tcacgttcac ggggcctaac cagacggcgg cagcggcggg aaccgaagac ggcaagatca
      841 gaaccgtcac gcgcgatcag ccgaaagtgg gacgcaacga cgcctgcccc tgcggctccg
      901 gcaagaagta caagaagtgc tgcggcgcgt gatgcgcgtc gcagcagcgt cgggggaagt
      961 tctcgcgtct cccggtgaag acataattga taactgaaat gcgttttact cgaatcaacc
     1021 tcgcaccgtc cttcgtctct ctactcaacg cgtgcgtgtg cctgctgctc ttcggcgcag
     1081 gaggcacacc gcacgcgcag cttcatgcgc aggccacgcc gccgacggcg tcctcctccg
     1141 gcgtcaacct tccgaccgat acggcgcgcg gcatcgaact ctacaaagcg gccaactacc
     1201 gcgaggcaat cgaagtgttg cgcggggcag tgaaggagcg tagagaggac ggcgaggcgt
     1261 ggctgcatct cggcatcgca ctgagccgcg cggacgagaa caaagaggcg cgcaaggctt
     1321 tcgagaaagc gatcaagttg aaacccgacg ccccgcaggc gcacatcggc atggcctatc
     1381 tcttgctcga atcgggcaag ccgaaggagg ccgaacgcgc cgccacacac gccctcacgc
     1441 tcaatgcgcg caacgccgaa gcccactacg cgctcggcct catacacctt cgccagaacg
     1501 ctcccgcgaa agcgttggcc gaggccgatg cgacgctaca actcaaccct actttcgccg
     1561 gagccttact gttgaaggtg gaggcggcaa aagaagccta cgcggcggca tattccgagc
     1621 attttgagaa gttaaaaaaa ctaaagactg tgcccgaagt ctcgacggag gagcgtgcgg
     1681 cggtcaacaa cttgctgaaa gaggcgtcag cggctctgga ccgatacctc gcgctctttc
     1741 ccggcgcgcc caacgcgacg cgactgcgcg aagaagccga gacgctgcgc ttttattcgc
     1801 agccgcttaa caagagcggc gagccgttcg cttacccggt gaacgcgctg acgacgaagg
     1861 cgcagattac ttcgaagccc gaaccgctct acacggagca ggcgcggcac ggtgggattt
     1921 caggaacggt gcgactgagg atggtactcg ccttcgacgg gaaggtgcgg cacatccttg
     1981 tcgtgcaggg gttgggaggc ggcttgtcgg aaatggccgt cgaagccgcg cgcaaaatta
     2041 agttcactcc ggccaccaga gatggtcgtc ccgtctctca gttcgtgacg attgaatata
     2101 acttccacat ccgttgagtc ggcgttatcg agtcgcagct tatttcacag tcgtttaaaa
     2161 ctcctccgac gccgtacact ttaactctcc cgtcaggccg aacatttcat gagcgcaaac
     2221 atttcttcga caaacccggt cgtgcaggcc atcgcggcgg gcacggctcc acagacggct
     2281 cgtttgatgg cggcgcgcgg gatgctgccg cttgcggcgg aagagttgct cgaagtgctg
     2341 ttggtgttgc gctccgacga aaatcaggag atcgtcgccg ccgccaacga gacgctcgac
     2401 gaacaacctg cggagacgct gctttcagtc gcacaggcgg cggagacttc gcccgtcgtg
     2461 ctctcttatc tggcgactcg cgacggcgcg ccgagagagg tcagcgaagc cgtcacctta
     2521 aacaccgcga cgcccgacga agccatcgcc cgcatggctt ccgccacctc cgacggctcg
     2581 ctcctcgaac tcatctccgt caatcaacag agactcatcc gcgcgcccga gatcatcgaa
     2641 gcgttgctca ccaatcccgc gcgcactaac gacgccgaac ggcgtgcgcg cgaaacgaag
     2701 cgcgagtttt tcgagaagga gcgcggcgcg cagcagatcg cagacgagat gcgcgcgcgc
     2761 ggcatgtcgg ctgccgccga gttcgtcgaa gcggcggaat ccatcggcac cggctccggc
     2821 ttaacttacg acgatgcgtg gctgctcgcg caacacatcg aagtctccga cgccgacata
     2881 gacgattcgt ggctgccgct cgaaaggatg gaggagtttt tcacggagag cgaggaacag
     2941 cgcgccgcca atctcgaacg catcatcgcc gagacgaccg ccgaagccgg agaagtcgcg
     3001 cccgaacgca tctcgctcat ccgccgcgtg atgctgatga gcgtcaagga tcgcgtcaag
     3061 ctcgccatga aaggcgaccg cgaggcgcgc ggcatcctca tccgtgattc aaacaaggtc
     3121 gtcgccacgg gcgtcatcaa caacccgcgc atcacggaca aggaggttga aggcatctcc
     3181 gccatgcgca ccgtctccga cgaagtgctg cgcctcatcg cacagaatcg cgcctgggcg
     3241 cgcaactatc cggtcatcca caatcttgcg cgcaacccgc gcacgcccat cgccaccgcc
     3301 atcaacgtcc tgctccggct ccacaccaaa gacctccaac acatctcgca gaaccgcaac
     3361 gtctccgacg ccgtgcgccg ccaagcccaa cgcctcgccc agacgcgcac cggacactaa
     3421 gacagtgacg agtgacaggt gacgagtgac aagttaaaga taagtaaatc gtgaatcgtg
     3481 aatcgtgaat agttaaaagc agttctctct ctatttacga ttcacgattc acgatttacg
     3541 ttttttcccg tcactcgtca cctgtcactc gtcactcaaa agatttccga gcctttgcgc
     3601 aggacagacc atgcgacatc tgtgagggtg tggctggctg cgccccacca gagtcctgcg
     3661 agcacggcga gcgcgacgtg gcggcctgtg ccttctatcc tcgcgcctgc ttcgatggcc
     3721 tgccaggcgc gcacgagttc gccgaagtcg ggcggggcag cggcaacgag cagcgcacgc
     3781 aggtagatgg cagagacggc gatgagcgat agcatcaacg cgaaataaac gacgcggatc
     3841 agcgtgccga agatgatgcc gtgcgaccag cgcgaacgat ggcgaaacat cactttgtaa
     3901 ggccaccaga tgaaacgaaa cacgccccag cgcgtgtatt gccgcgactg tatatcgagg
     3961 tcagg
//
LOCUS       DASWNS010000531        15041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_460753, whole genome shotgun sequence.
ACCESSION   DASWNS010000531 DASWNS010000000
VERSION     DASWNS010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15041
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460753"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..613
                     /locus_tag="VGB76_18215"
     CDS             176..613
                     /locus_tag="VGB76_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100888.1"
                     /translation="MRHATPQLRELARRLFAHEAKKSRSPAALAGVMEESCRRLHARL
                     DPLIGAGGFRALLARALHLAAKEIPWLAAVGVEEHPACALEGLRNAVKGQDAAAVREA
                     FALVLANIIWLLVTFIGEDIAFGLVKEVWPEAEKVSTSEEGNR"
     gene            614..2314
                     /locus_tag="VGB76_18220"
     CDS             614..2314
                     /locus_tag="VGB76_18220"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY0100889.1"
                     /translation="MSKNGKVTIERLPTGVPRLDDVLGGGVPEFSFNLIAGDPGSGKT
                     TLTHQIIFANATEERPAIYFTILGEPALKMLRYQQQFTFFDMAKVNKAIHFINLSQEA
                     MEKDLSKVLETIVGHVERINPAIVVVDSFRTLVRVTNVSEPGQMHLSEFVQRLAMHLT
                     SWQVTSFLIGEYEEQEMRENPVFTMADGIVVLTQSVDRNSMVRKLRVPKMRGQSPQPG
                     LHSARINDDGVQIYPRMIKPVVDSQQENLPSRMISTGISGLDEMLGGGTLTGNAILIA
                     GPAGAGKTTTAIQFINEGVTQGEPGVMALFEETTPKYLDQAKSFGFDLEKMCEQGLLE
                     MVYIRPLDLSVDEMLYAIQEAVDRVGAKRVVMDSISALQAALAPTFEQDFRESLYRLL
                     GAMTGVGITVMMTVELTENYNELRFTPHIVSFLTHDIVLQRYIEMEGQLRRMITVVKT
                     RGRKHSSDLRSYEVTGKGIVVGEVLKQYRGLITSVPELREAVEPLARRGLTDQEAMVF
                     QTLRESGDALFTDLSKGTKLKRAELTRSLERLVSLGFAKKVKRKGETVYRQTDEQKND
                     "
     gene            complement(2365..4392)
                     /locus_tag="VGB76_18225"
     CDS             complement(2365..4392)
                     /locus_tag="VGB76_18225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0100890.1"
                     /translation="MSTTISPRLSLGNNLLAALPEDGYERLLPHLEYVELRLGEVLHE
                     PDETIRYAYFPVNSVVCLMALMESGGTVAVGIVGREGIIGLPLFLGGNTTSNQAVVQV
                     GGGAVRIKAEVFRRECDESGALRDLLHLYTQALLTQTSQAAACSRLHKLKHGLARWLL
                     AVHDRVDGDEFKCTQELLAGIMGVRRAGVTEAALELQDEGVIGYTRGLIHVADREGLE
                     LAACECYELVKGEFDRLLTTELGGKRVSAKPYAWNGVKRRHGELNGKKFETLREINSR
                     LLMAAVREQEARDEAEEANQAKDEFLATLSHELRTPLTAMLGWSRMLRTTKLDEAVSA
                     HALEIIERNAEAQVRLIEDMLDVSRIIAGKLRLEVQPFEPESVIKTAVDAVRPAALAK
                     GIQIKCALDSKPTRFSGDQKRLHQVVSNLLTNAVKFTPEGGSVEVRLESTDALVWLSV
                     SDTGQGISPEFLPHVFDRFRQADSGTTRTSGGLGLGLAIVRHLVELHGGTVRAESRGE
                     GQGATFTVELPLTLGQSETGDAQRSAEKTKDVKGAFECSGSLEGLHVLVVDDEDDTRE
                     LLAFVLEQCGAKVTAVASARQALDALARSKPHVLLSDIGMPGEDGYELIRQVRASVRE
                     LGRKIPAAALTAYTTEGDRERVLSAGFQVHISKPVEPSNLVAIVAHLAGRI"
     gene            5257..5631
                     /locus_tag="VGB76_18230"
     CDS             5257..5631
                     /locus_tag="VGB76_18230"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0100891.1"
                     /translation="MEPFDVTRIREDFPVLKREVQGKPLVYLDNAATSQKPQAMISRL
                     SELYSHEYARVEEGHTLSREATEIFEGTRAKVAKLINAAEAREIVFCRGATEALNLVS
                     RCFEQDSLQAGDEVPHLVRRRR"
     gene            5597..6016
                     /locus_tag="VGB76_18235"
     CDS             5597..6016
                     /locus_tag="VGB76_18235"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0100892.1"
                     /translation="MRCLISFVVEGKKPKEVEKALDAEGIAVCAGKLAAEPLLKALGA
                     DEAVRASFMFYNTRQADAQILSRHIVCHSTGQVSERHFHQFYRDPLARAAVVSEIVAA
                     ALRNQLEGAYPSPAKAFGTELSRLLPEPPVVVGSVEV"
     gene            6517..7341
                     /locus_tag="VGB76_18240"
     CDS             6517..7341
                     /locus_tag="VGB76_18240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0100893.1"
                     /translation="MAVAASTQQGKIESRWITVGGLRMHARVSVNLPPASACDVPVVL
                     VHGLVVSSLYMVPTAERLAPFFRVFAPDLPGFGKSDKPARALNVPELAGALVAWMDAA
                     GLVQAVLVGNSLGCQVIAHVAAHSPQRVAGLVLAGPTVDPAGRTLSEQFKRLLLDAPH
                     ERFSILFAELRGLSQAGIKRSWQTARLELEDHIEEQLPHIRAPALVVRGTRDPVVPQE
                     WAEQAVRLLPRGRLAVVAGGPHGLNYTTPDELARLIREFIERDVSPACDAAGIQGE"
     gene            7430..8005
                     /locus_tag="VGB76_18245"
     CDS             7430..8005
                     /locus_tag="VGB76_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100894.1"
                     /translation="MRHPFESIPRGVRGRLLLPLLAATLLILVIWVVMVSPMSNERAP
                     LSVSSFGMAGSLERAREMMASWDERARLSGVFGIGFDFLQLVVYSTTVAMACAWVAQS
                     FRENGHRGLSSIGVLLAWGQWLAVICGTVQNAVMMSLLLGSGSEAWLGISYWSTLLKL
                     LLLILGPAYALAGWLRLRWTGFAIPRLPEVG"
     gene            complement(8012..8410)
                     /locus_tag="VGB76_18250"
     CDS             complement(8012..8410)
                     /locus_tag="VGB76_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100895.1"
                     /translation="MTEELKNQPIIFLLEADDDTRPILKSNLQRYGYRIIIALDEEDA
                     LDRVSGGLRADLILVDTVGVSLEEALGTGRRIRTHAERNGHTPLVVMAEKYGADVEGT
                     NVNVGGNDWVTYLEDPDQLKNLLARLTAPA"
     gene            complement(8492..9199)
                     /locus_tag="VGB76_18255"
     CDS             complement(8492..9199)
                     /locus_tag="VGB76_18255"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0100896.1"
                     /translation="MSEALRPQNQNHILAALPAEDYQRLSAHLEPIELRHSQVLYDAG
                     GAMDYVYFPLGSMVSLISQLSDGSSVEIGITGYEGMVGVSAMLGVDRSPHEAMVQIPD
                     GALRVKTGVLKEEFKRGGALHDSLLRYLQMLLLQTGQIAACNRLHSIGERLARWLLMS
                     HDRCHCEELPFTQEFLALMLGIRRAGVTEAALILQAEEYISYRRGQITILDRAGLEEF
                     TCECYQIVKDEFDRLVG"
     gene            complement(10140..10505)
                     /locus_tag="VGB76_18260"
     CDS             complement(10140..10505)
                     /locus_tag="VGB76_18260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0100897.1"
                     /translation="MPPTKKRILCVDDHSDNCELISIILKDYEVTSAYSMADALKRAT
                     AERFDLYLLDYHLPDGTGLELCLMLRAFDRDTPILFATASSSLTESQVITVGAQGLVK
                     TGLGLADDLTARVSRLLQA"
     gene            complement(10748..11536)
                     /locus_tag="VGB76_18265"
     CDS             complement(10748..11536)
                     /locus_tag="VGB76_18265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEY0100898.1"
                     /translation="MALVCGGAGWALPYTHFYTDDSRTVAMSWRRRDIPVYLSTSILT
                     PPANIKAGSDVEGAVRRSLRRWEQASGVRFVTNLTPEQSASARRNGDRINLITVADTP
                     ENSSFFSGALIGYTKLFFDRRTGEISEADIIINARQKFSTDGSPGTYDLESTLTHEVG
                     HLLGLDHSAVHGAIMREGQAINDGTGRSHFPGRELGEDDRAGVRSIYGESADALSLDP
                     GTVMSQKIFWPQLVELKRHLPPRYLVSSESSCNPTSRLFGLRPA"
     gene            12349..12903
                     /locus_tag="VGB76_18270"
     CDS             12349..12903
                     /locus_tag="VGB76_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100899.1"
                     /translation="MKQKLGLVLALMLVAGASAMLVAQSQTGTQTGTQTGTQAGAQGN
                     RSGTQTNANTGASGSQNMTGSTQSNGATSTGQGQTGTTGAGATGTGTMGTQGTTGSGA
                     GASGSRMGQGGQTGSQNNGMGTSGAGGGNLGTTGGGQTGTGGTTGGGMTGSGMDAMGT
                     TGAGAQGTDDLGGGTTGRNRMFKH"
     gene            complement(13132..14154)
                     /locus_tag="VGB76_18275"
     CDS             complement(13132..14154)
                     /locus_tag="VGB76_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100900.1"
                     /translation="MAAIRKQRGLLYGATLVSALILCGVFALVRQVGHFGRVPPPAAL
                     AERSTAVSVETHAATATPEVRPRSVRFERLAEIGRGIGVIFSPDFSAPANRRFYERLG
                     FAYFEGADWRDLLAQVRAYNRAHPGRRIETLILEVHGTNGHGLKLQASSAPRVPRSYI
                     SVGGLQQQLADAGLRLCVIAACNSGRLFRPEIYYTLNTRTGDPLFLPATLGVVNAAPD
                     FRATERDVRVVYPVTSRLEAANEGHLSEFAPLTVALLNGDPKARATDSTKVAADARFV
                     VSDTLLALLLNDPGLNLTADAYVTQMSAATSSPAESESLVQRFIALVDEAAAREYSAA
                     HNGGGG"
     gene            14448..>15041
                     /locus_tag="VGB76_18280"
     CDS             14448..>15041
                     /locus_tag="VGB76_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100901.1"
                     /translation="MKKSLKTSLIVIALALLACLVWLGLRPDAPDAPVGASVPETARG
                     LSFEVHVEKPRLNRFLFGILPTKLEDKLFGSGELRFDHASPGAGIGSIGHNRLELRAD
                     GWHLLMETDGEGKVAPGTSLVFPILLAERQRTLRCRPADRATGYLDAATRAGSDVLDG
                     RFLVELATCENAETGKVIEWPQAPLTVRGSFAGLPHGH"
BASE COUNT         3103 a         4475 c         4550 g         2913 t
ORIGIN      
        1 catgtcgagg aaatgatcac ctgaaagatt aagcgccaat cttactaaac accgggaagc
       61 aggactgtac gtttcagcac ccacaataca tgatcccgaa aattcatctt ctccgccgga
      121 aatctgctat gctggccgca cttgtgaaga ctcccgtaga gaagactgcg agcgaatgag
      181 acacgccacc ccccaattac gtgaactggc acggcgtctg tttgcgcacg aggcgaagaa
      241 gtcacgaagc ccggctgctc tggccggggt gatggaagaa tcctgccgtc gtttgcacgc
      301 gcgactcgac ccgctcatcg gggccggggg ctttcgtgcg cttctcgccc gcgccctgca
      361 tctcgccgca aaggagatcc cgtggctcgc cgccgtaggg gttgaggaac atcccgcctg
      421 cgcgctggaa ggcttgcgca acgcggtgaa gggacaggat gcggcggcgg tgcgcgaagc
      481 ttttgcgctg gtgctggcga atatcatctg gctgctggtg acgtttatcg gggaagacat
      541 tgcgttcgga ttagtgaagg aagtgtggcc ggaggccgaa aaagtttcca cctcagaaga
      601 gggaaataga tagatgagta agaacgggaa agtaactatc gagagactgc cgacgggtgt
      661 gccccggctc gacgacgtgc tcggcggggg cgtgccggag ttttccttca acctgatcgc
      721 gggcgacccc ggatcgggca aaactacgct cacgcaccag atcatcttcg ccaacgccac
      781 cgaagaaaga ccggccatct acttcaccat cctcggcgag cctgcgctca agatgctccg
      841 ctaccagcaa cagttcacct tcttcgacat ggcgaaggtg aataaggcca tccatttcat
      901 caacctcagc caggaggcga tggagaagga tttgagcaag gtgttggaaa ccatcgtcgg
      961 ccacgtcgag cgcatcaacc cggccatcgt ggtggtggat tccttccgca cgctcgtgcg
     1021 ggtgaccaac gtctcggagc cggggcagat gcacctctcc gagttcgtgc agcgtctcgc
     1081 catgcacctg acgagttggc aggtgacatc gtttttgatc ggcgagtacg aggaacagga
     1141 gatgcgcgag aaccccgtct tcacgatggc cgacggcatc gtagtgctca cgcagagcgt
     1201 ggatcgtaac tcgatggtgc gtaagctgag ggtgccgaag atgcgcggcc aatcgccgca
     1261 gcccggcttg cactccgcgc gcatcaacga cgacggagtg caaatctacc cgcgcatgat
     1321 caagccggtc gtagactcgc agcaggagaa ccttccttcc agaatgatct cgaccggcat
     1381 ctccggctta gacgagatgc tcggcggcgg cacgctgacc gggaacgcca tcctcatcgc
     1441 aggcccggcc ggggcgggta agacgacgac ggccatccag ttcatcaatg agggggtgac
     1501 gcagggcgaa cccggcgtga tggcgctctt cgaggagacg acgccgaagt atctggatca
     1561 ggccaagagt ttcggtttcg atctggagaa gatgtgcgag cagggcttgc tggagatggt
     1621 ctatatccgc ccgctcgacc tctcggtgga cgaaatgctc tacgcgattc aggaggcggt
     1681 ggatcgggtg ggagccaagc gcgtcgtgat ggattcgatt tcggcacttc aggccgcgct
     1741 cgcgcccacg ttcgagcagg acttccgcga gtcgctctac cggctcttag gcgcgatgac
     1801 cggcgttggc atcaccgtca tgatgaccgt cgagttgacg gagaactaca acgaactgcg
     1861 cttcaccccg cacatcgtct ccttcctgac gcacgacatc gtgctgcaac gctacatcga
     1921 gatggaaggc cagttgcggc ggatgatcac ggtggtgaag acgcgcggca ggaagcacag
     1981 cagcgaccta cgttcttacg aggtgacggg gaaaggcatc gtcgtgggtg aggtcttgaa
     2041 acagtatcgc ggactcatca cctcagtccc cgaactacgg gaagcggtcg agcctctggc
     2101 gcggcgcggg ttgaccgatc aggaggcgat ggtgtttcag actttgcgtg agtcgggcga
     2161 cgccctcttc acagacctgt cgaaggggac gaagctcaaa cgcgcggaac tgacgcgctc
     2221 gctcgaacgt ctggtgtcgc tcggcttcgc caaaaaagtg aagcggaagg gcgagacggt
     2281 ttacaggcag actgacgaac agaagaacga ttagcttcct ctctcactaa gattcacaga
     2341 cgccttttcc gcactctcgc ttcctcagat tcgcccggcg agatgggcga caatcgcaac
     2401 cagatttgac ggctccacgg gtttcgagat gtgcacctgg aaccctgccg agagcacgcg
     2461 ttcgcggtcg ccttccgtgg tataagccgt cagggcagcc gccggaatct tgcgcccgag
     2521 ttcacgcacg gaggcgcgca cctgacggat caattcgtaa ccatcctcgc ccggcatccc
     2581 gatatcgctc aacaggacgt ggggcttaga tcgcgcgagg gcgtcgagcg cctgacgcgc
     2641 agaggccacc gcagtaactt tcgctccaca ttgttcgagc acaaaggcga gcagttcgcg
     2701 cgtgtcgtct tcgtcgtcca caaccagcac atgtaagcct tcgagcgacc ccgagcactc
     2761 gaatgcgccc ttcacgtctt tagtcttttc ggcagaccgc tgcgcatcac ccgtttccga
     2821 ctggccgagt gtgagcggaa gctcgacggt gaaggtcgcc ccctgccctt cgcccctgct
     2881 ctccgcacgc accgtgccgc cgtgaagttc cacgagatga cgcacgatgg caaggccgag
     2941 gccgaggccg ccggacgttc tcgtcgtccc actgtcggcc tgacggaagc ggtcgaagac
     3001 gtgcggcagg aattccgggc tgatgccctg cccggtgtcg ctcacgctga gccagacgag
     3061 tgcatcagtg ctttcaaggc ggacttcgac cgacccgccc tcgggcgtga acttaacggc
     3121 gttggtcagc aaattcgaca ccacctgatg caggcgtttc tgatcgccgc tgaaccgagt
     3181 cggcttagaa tcgagcgcac acttaatctg aatgcctttg gcgagagccg ccgggcgcac
     3241 ggcatctacg gcggtcttga taaccgactc gggttcgaac ggctgaacct caagccggag
     3301 tttgcccgcg atgatgcgcg aaacgtccag catgtcttcg ataagtcgca cctgcgcctc
     3361 ggcgttgcgc tcgatgattt cgagcgcgtg ggcggagacg gcctcgtcca gcttcgtcgt
     3421 gcgcagcatc cgcgaccaac cgagcatcgc cgtcagcggc gtgcgcaatt cgtgcgagag
     3481 cgtcgccaga aactcatcct tggcctgatt agcttcttcc gcttcgtcgc gcgcctcctg
     3541 ctcacgtacc gcggccatga ggaggcgact gttgatctct cgcagcgtct cgaatttctt
     3601 accgtttagc tcgccgtgcc tgcgcttgac gccattccac gcgtagggtt tcgcgctcac
     3661 tcgctttcct cccagttcgg tagtgagcag ccggtcgaac tctcccttga ccaactcgta
     3721 acattcgcaa gccgccagtt cgagtccctc gcggtcggcc acatgaatga ggccacgggt
     3781 gtagccgatc acgccttcat cctgtagttc gagcgcggcc tcggtgacac cggcgcgccg
     3841 gacgcccatg attccggcga gcagttcctg cgtgcattta aactcgtccc cgtccacgcg
     3901 atcatggact gccagcagcc aacgcgccag cccgtgcttc agtttgtgca ggcggctaca
     3961 ggcggcggct tgtgaagtct gtgtcaggag cgcctgcgtg tagagatgca ggagatcgcg
     4021 gagcgcgccg ctttcgtcgc actcccgtct aaacacttcc gccttgatcc tcaccgcgcc
     4081 gcccccgacc tgcacgacgg cttgattgga ggttgtgttg cctccgagga acaagggcaa
     4141 gccgattatt ccttcccggc ctacgatccc gaccgcgacc gtgccgccgc tctccatcag
     4201 cgccatcagg cagacgaccg agttgacggg aaaataagcg tacctgatcg tctcgtcggg
     4261 ttcgtgcagg acttcgccga gccggagttc gacatattcc agatgtggca gcaggcgttc
     4321 atacccatct tcgggaagag cagccagcag attgttgccg agcgacaagc ggggcgagat
     4381 ggtcgttgac ataatgaacc tctgatcttt gtcgggttgg ggtgaagtaa gtggtgaaga
     4441 gagagcttgt ggggaagctc aatccgtgtc cagcgaatgg taaaacttct taaccccggg
     4501 tctagtaggg acgcactata tcacaggagg ctgcggttca gcccatccgg ctcgaagtga
     4561 cgctcttgga aagacgagga gttgcgtttc atggatagac ctgtcgagta gaattgagag
     4621 agcacaacgg atgaacaata cccggatggc tactctcgcc acactcgcct gatctctaaa
     4681 tcgccgctcg cgccccgcgc gcttcccgtt cgtaaacgta cagacggctc tcgtgagggc
     4741 tacgtatagt caatttcccc tacttataaa ctttaatgaa gacgctggtc agacgatgac
     4801 gaccagcgcc gcgcggtgtc ttgaagtacg tgcgggtcaa tgcgtccggc cttttacgaa
     4861 ctcgaccacg gaagcatgcc cgcgagttaa ggcgtacaag gatggacgcc gcaccgaact
     4921 ggcagggagg aaagttttgc gatggaaacg cacacaaccg aacgaatgac ggatagagat
     4981 ttcaggcggc tcctggagtc ggggcggcat acgaagacga cctgcccggg gtgcgggcaa
     5041 tcatggctca tcttaggcgt aggcagacat gacacgcaca cttgcaaggc gtgcggccac
     5101 ggatttcgcc tcacagcctc ttcgcgtcag ggacacgctc cgcgtcaggg cgattaaagc
     5161 aaagcgacaa acggaagccc caactaccat cccggcgggt atacaacagg gacggctcga
     5221 tgcagagaag gttagaaaca ggaggagtga taagacatgg agccatttga cgtaacgcgt
     5281 atccgcgaag actttcccgt tctgaagcga gaggtgcagg gcaagccgct cgtctatctc
     5341 gacaacgccg cgaccagcca gaaaccgcag gcgatgatca gccgtctgag cgaactgtat
     5401 tcgcacgagt atgcgcgcgt cgaagaggga cacacgctca gccgggaggc gacggaaatc
     5461 ttcgagggca cgcgcgcgaa ggtcgccaag ctcatcaacg ccgccgaagc gcgcgagatc
     5521 gtcttctgtc gcggtgcgac cgaagctctc aacctcgtct cgcgctgctt cgagcaggac
     5581 tctttgcagg cgggcgatga ggtgcctcat ctcgttcgtc gtcgaaggta agaaaccgaa
     5641 agaggtcgag aaggcactcg atgctgaggg catcgcagtc tgcgcgggca aactcgctgc
     5701 cgaaccgctg ctcaaggcgc tcggcgctga cgaggctgtg cgagcgtcct tcatgttcta
     5761 caacacgcga caggctgacg cgcagatttt gtctcggcac atcgtatgtc acagcactgg
     5821 tcaggtatcc gagcgccact ttcatcagtt ctatcgtgac ccactcgccc gcgcagcggt
     5881 ggtgtctgag atagtcgccg ccgccctgcg gaatcaactc gaaggggctt acccctcgcc
     5941 cgcgaaagcg ttcgggacgg aactctccag gctgctccca gagccgcccg tcgtggtcgg
     6001 ttccgtagag gtctaacatc accgacctgc ctttcgggaa gcggtatcct ctccactcga
     6061 actcccgtct gacgatggcg gcgatgaagg ggaagaaagg gtaaaagcgg cgaacttcct
     6121 gcacgaacaa ctcgtcgtaa ccctcttcgc ccgacaagag ttgctctcgg ctctccgggt
     6181 gttcatgcag cgcgagagcc aggaaggtga taaaccgcgc gacagcatga gccgggtttc
     6241 aaagatgtcg gagcgatagc gcctgaacct tttcgccgta aagttgtacc cttcaagaaa
     6301 cagcgccagc gagctgtcag gacttttgtc gcgcggaatc tgcgccattg aaccctccca
     6361 ctaccttcca taacataaag gtatggacag accgatttca tctgtaagga agaagtttcg
     6421 ctcagttgtt gtgataagtt tacgaacaat ccgatcaata acaccatgac gcacggctga
     6481 tgaagccgca atagaaacct gtcgatgcga ccggagatgg cagtggcagc gagcacacag
     6541 caggggaaga tcgagagccg atggattacg gtcggcggct tgaggatgca cgcgcgagtc
     6601 tccgtcaacc tgcctccagc gtcggcgtgc gatgtgcccg tcgtgctcgt gcacgggctg
     6661 gtcgtctcca gcctctacat ggtgcccacg gcagaacgac tcgcgccttt cttccgcgtg
     6721 ttcgcccctg acctgcccgg cttcggcaag agcgacaagc ccgctcgcgc gctgaacgtg
     6781 ccggaactgg ccggtgcgct tgtcgcgtgg atggacgcgg cgggactcgt tcaagccgtt
     6841 ctcgtcggca actcgctcgg gtgtcaggtt atcgcccacg tggcggcaca cagtccgcag
     6901 agggtagcgg gcttggtgct ggccgggccg acggttgacc ccgcagggcg caccctcagc
     6961 gagcagttca agcgcctgtt gcttgacgcc ccccatgagc gattctcgat cctgtttgca
     7021 gagttgcgcg gcctctctca agcggggatc aagagatctt ggcagacagc ccgactcgaa
     7081 ctcgaagatc acatcgagga gcagctaccg cacatccgtg cgcccgcgct cgtggttcgc
     7141 ggcacgaggg acccggtagt gccgcaggag tgggctgaac aggctgtcag gttgctgcca
     7201 cgcgggcgtc tggcggttgt tgcgggaggc cctcatggcc tcaactacac gacgcccgac
     7261 gagttagccc ggctgatacg cgagttcatc gagagggatg tttctccggc ctgcgatgca
     7321 gcgggcatac agggagaatg aagatgatac tttcaccctc gcggttcaaa gtcacccgac
     7381 gaagggctgc gtgcgttaca agtttcgaca accgacaagg agggtattga tgagacatcc
     7441 attcgaaagt atcccgcgcg gcgtgcgtgg gcggctgttg cttccgctgc tcgccgccac
     7501 gctactcatt ctggtgatct gggtcgtcat ggtcagcccg atgtcgaacg agcgtgcgcc
     7561 gctgagcgtc tcgtccttcg gcatggcggg cagccttgag cgggcgcgcg agatgatggc
     7621 ttcgtgggat gaaagagcgc gcctgagtgg tgtcttcggc atcgggttcg acttccttca
     7681 actggtagtt tattcgacga ccgttgcgat ggcctgtgcg tgggtcgcgc agtcatttcg
     7741 ggagaatggt cacagggggt tgagttctat cggggtgctg ctcgcgtggg ggcagtggtt
     7801 agcggtcatc tgcggaaccg tgcagaacgc cgtcatgatg tcattgctgc tgggatcggg
     7861 tagcgaggca tggctcggaa tctcctactg gagtacgctc ctgaagctgt tgctgctgat
     7921 ccttggtccc gcttatgcgc tggctggttg gttaagatta cgttggacgg gatttgcaat
     7981 tccgcgcctt cccgaagtcg gttagaactc tttatgccgg ggcagtcagc cgggcgagta
     8041 gattcttcaa ctggtctggg tcttccaggt aagtgaccca gtcgttgccg ccgacgttga
     8101 cgttcgtgcc ttccacgtct gccccgtact tctccgccat caccaccagc ggcgtgtggc
     8161 cgttacgctc cgcatgggtg cggatgcgcc gccccgtgcc gagcgcctct tccagcgaga
     8221 cgccgaccgt gtccaccaga atcaagtccg cgcgcaggcc gccgctcact ctgtccaagg
     8281 cgtcttcctc gtccagcgcg atgatgatgc ggtagccgta cctctgcaaa ttgctcttga
     8341 gtatggggcg tgtgtcgtcg tccgcttcga ggagaaagat gattggttga ttttttaatt
     8401 cttcggtcat actgccctgc ccctgacttc aaaagagact ctgacacgca actcctgggg
     8461 agagctactc aatccatgtc gtcacgtcgg cttaaccgac taagcggtcg aactcgtcct
     8521 tcacgatctg gtagcactcg caggtgaact cttccagccc cgcccggtcc agtatcgtaa
     8581 tctgacctcg ccggtaactg atgtactctt cggcttgcag gatgagtgcg gcttcggtca
     8641 cgcccgcacg ccggatgccg agcatcaagg cgaggaactc ctgcgtgaac ggcaactctt
     8701 cgcaatggca cctgtcatgg ctcatcaaca accagcgcgc gagtcgctcc ccgattgagt
     8761 ggagccggtt acaggcggcg atttgacccg tctgcaacag caacatttgc aggtagcgca
     8821 acaatgaatc gtgcaacgcg ccgccgcgct tgaactcttc tttcaagacg ccggttttca
     8881 cccgcagcgc accgtcggga atctgcacca tcgcttcgtg cggcgagcgg tcgacgccta
     8941 acatggcaga gacgccgacc atcccctcgt acccggtgat gccgatctcg acgctcgacc
     9001 cgtcggagag ttgcgagatt agagaaacca tcgagcctaa agggaaatag acgtaatcca
     9061 tcgctccccc ggcgtcgtag agaacctggc tgtgacggag ttcgatgggc tctaaatgtg
     9121 cgctcaggcg ttgataatcc tctgcgggca gggcggcgag gatgtggttt tggttttgcg
     9181 ggcgtagtgc ttctgacatc aggactttct ctttcatgcg acggtcgcgt gtgaaaaggc
     9241 agcagacggc acgaggagaa ggacataaat ctcttccgga tgtcgaatgt tgagttggga
     9301 aataaccggg agtagctaac gtgagcagta taacatcaac tgcgcgcttg ccacccgata
     9361 ccgtacggaa agcaccggag gagcacgaat ctccccggcg acattcaccc gccgattaac
     9421 tttccgtagg caatggaacc atatattttt atgattccgc gctatgattg ttgaaaaaaa
     9481 tgattgagtc agagcgcgga gcagtcagcg tctccgactg cacggagttg gaagcgcaag
     9541 ccagcgagcg tcagaccgtc aaccggaaag ggtttgacac cttcgtctct ctaacccaca
     9601 accgacgcga ctcgacagct agctcatctc gtaccaagtc gtacagacaa tcaacgccgc
     9661 tcgccgctat aaatcatctt gcttaacgag caagggaaag cagtgcaggg ctgccgggta
     9721 ggtcgcacat agttgtcgcc cccgatgggc ctactgcatc acacgcgccg tacccgacac
     9781 aggctgccga cagacgactc cctccatgat gcagaactct aaaattcgcg tcctcttcgc
     9841 tgacgatcac gaagacatcc gcctgttggt gacgaccctg ctcggcgcgt ccggctatca
     9901 ggtcgtcacc gcgagcgact gcgcgagttc gaccgcgaca cgccggttgt cttcttctcc
     9961 ggcgaagcct tcgaatccga caggcagggg gcgctcagtg tgggcgcgca gggctacgtc
    10021 gagaagccca acgtctcggc cttgccgcga gctatcgcaa gcgtcctgcg taccgcgtcg
    10081 caagcacact aaactcgaag cataatttta agtttgaatg ttgtaagact gttcggactt
    10141 caagcctgta gcaggcggga tactctcgcc gttaaatcgt cggccagacc cagtcccgtt
    10201 ttcaccaatc cctgcgcgcc gaccgtgatg acttgcgact cggtgagcga actggatgcg
    10261 gtggcaaaga ggatcggggt gtcgcggtcg aaggctctga gcatcaggca tagttcgagt
    10321 cctgtcccgt ccggcaggtg gtaatccaaa aggtagaggt cgaaccgctc ggcggtggcg
    10381 cgtttcaggg cgtcggccat gctgtaagcg gacgttactt cgtagtcctt caggatgatc
    10441 gagatgagtt cacaattgtc ggagtggtcg tcaacacaga ggatccgttt tttagttggg
    10501 ggcaaacatt agtccttcct cataacagac ggcgttacga ggcggcgcaa tgttatgtgc
    10561 gcccatattg cgcctcattt gtgaagaata caagggacgg gttttggatg ccctacagat
    10621 cgagccggga gggacgttaa agtcagcatg aatcacaacg agcagttact acggaaggca
    10681 gcgatttacg gggctatact tttgacgatc atcttcacca ctttactgtg gaaggggtaa
    10741 tcagcactta tgcaggtcga agcccgaaca gacgcgaggt agggttgcaa gaactttcag
    10801 acgacacaag atatcgaggc ggcagatgac gcttcaactc aaccaattgc ggccaaaata
    10861 ttttttgtga cattaccgtt ccgggatcta gagaaagagc atcggctgat tcaccataga
    10921 tggagcgcac cccggcgcgg tcgtcttcac ccaactcgcg tccgggaaaa tggcttctcc
    10981 ccgtgccgtc gttaatcgcc tgtccctccc gcatgatggc gccgtgcacg gctgaatgat
    11041 ccagcccgag caggtgtccc acttcatgcg tgagggtgga ttcaaggtcg tatgtgccgg
    11101 ggctgccatc ggtcgaaaac ttctgccgtg cgttgatgat gatgtcggcc tcgctgatct
    11161 ctcccgtgcg gcggtcgaag aaaagcttcg tgtagccgat gagcgcgccc gagaagaacg
    11221 aactattctc cggcgtgtcc gcgacggtga tgagattgat gcggtcgccg ttgcgccgtg
    11281 cgctcgccga ctgctcaggc gtcaagttcg tgacgaaacg gacgcccgaa gcctgctccc
    11341 aacgtcggag cgagcggcgt acggcgccct ccacgtcgct ccccgctttg atgttcgcgg
    11401 gcggcgtcaa gatagacgtg gacagataaa cggggatgtc tcgacgccgc caactcattg
    11461 ccaccgtcct gctatcgtct gtgtagaagt gtgtgtaggg caaagcccat ccagcgccgc
    11521 cgcacaccag tgccacggcg cacataaatg tgaaccacag tttgcgcatc tgattgtcta
    11581 aagccgagtt gaggagactt gcgggagggt cagttttgac tatgcagctt tctttcccgc
    11641 ccctgaaatt ctttcatccg tcgcagaaga gccggggcgc tgaccgttgg cctcacccca
    11701 cggcctgccc tcgcagacgc aggctgacat gcgcttgaac gcgcccgctg tcgcaccagc
    11761 ctcgggtgcg taaacactcg ccccgcactc tttacaaata ccgatcttcg tataaccggg
    11821 gacacccccc tgttctttac tcacgaccca ctccttattc attaccctgc aatcgggggc
    11881 acacttccgg cgacctttac tctctcttaa cctgtgcgcc ccgacgccct tgaaatcaaa
    11941 gggaaccgga ttcacccgat ccggcacccc gcacaaattc actccgagat cactcaaaga
    12001 aggatttatc taacattgct ttagatgtcc ggtgagacgc gtctgttgta cggttgcgta
    12061 caagtcgctg ggtgaatagg ggacgacggg cagttggctt caccggaagc gcgggaaact
    12121 tttacgtaga gagacaggtg gaagagagac ggttgatgtc tgctaattca atcaaattag
    12181 taacaaaacc gttacccgcc gtgttgtgtg cgcatccgaa caaacagctc gccgcattgc
    12241 atgtattcta tgtttaccga atccaatcta ggcacggcac cctgtccggc aggggcgtga
    12301 atgaaatttc agtcacaggt agtagggatt tacaaggagg ggaaattcat gaagcagaaa
    12361 ctagggctgg tgctggcatt gatgctggtg gcaggcgcga gcgcgatgtt ggtggcgcag
    12421 agccagaccg gcacgcagac aggtacacag acaggtacac aggcgggtgc tcaaggcaac
    12481 aggagtggca cacagactaa cgcgaacaca ggcgcgagcg ggtcgcagaa catgaccggt
    12541 tcgacgcaga gcaacggtgc tacgtcaacc ggccagggac agaccggcac caccggagcg
    12601 ggagcgacgg ggacgggcac gatgggcacg cagggaacga ccggcagcgg cgctggcgca
    12661 tccggctcgc ggatgggtca aggcggacag accggcagcc agaacaacgg catgggaacg
    12721 tccggcgcgg gcggcggcaa tttgggcacc accggcggcg ggcagaccgg cacgggcggc
    12781 acgaccggcg gcggtatgac cggcagcggc atggacgcaa tgggcacgac tggcgctggc
    12841 gcgcagggca ccgatgatct gggtggcggc acgacgggtc ggaatagaat gttcaaacac
    12901 taattcgcgc cgcatggcag cgatgccagc ggtgccagcg ttgatagagc ggggcggcat
    12961 ccgagccaca ctttagcgcc ctgccacaga cctacatcaa cgcgcaggct caggctaaca
    13021 atgcgcgaag ggcagccgaa tcaaacatcg gctgcccttc gcgcattgcg gcttttttcg
    13081 tctcaagcgg tcgccgtgga agctttcgcc gcccacaggc cgacgacatt atcatccacc
    13141 gcccccgttg tgagccgcac tgtactcacg cgccgccgct tcgtcaacga gggcgatgaa
    13201 acgctgcacc agactttcgc tctccgccgg agaggaagtg gcggctgaca tctgggtcac
    13261 gtaagcgtcc gccgtcagat tcagccccgg gtcgttcaag agcaacgcga gcagtgtatc
    13321 ggacacgacg aaacgcgcgt ccgcagcgac cttcgtactg tcagtcgccc gcgctttcgg
    13381 gtcgccgttg agcagggcta cggtgagcgg cgcgaattcg ctcaggtgcc cctcgttggc
    13441 cgcttcgagc cggctcgtca ccggatagac gacgcgcacg tcgcgctcgg tcgcgcggaa
    13501 atcgggcgcg gcattaacga cgccgagcgt ggccggcagg aagagcggat cgcccgtgcg
    13561 cgtattgagc gtgtagtaga tttctggccg gaacaaacga cctgagttac aggcggcgat
    13621 gacgcagagg cgaagccccg cgtccgcaag ttgctgctgt agcccgccga cggagatgta
    13681 cgagcgcggc acacgcggcg cgctgctcgc ctgaagcttc aggccgtgcc cgttcgtccc
    13741 atgcacttcg agaatcagcg tctcgattcg tcgcccggga tgcgcgcggt tatacgcacg
    13801 cacctgcgcc agcaggtcgc gccagtccgc gccttcgaag taggcgaagc cgagacgctc
    13861 atagaagcgc cggttggcgg gggcgctgaa atccgggcta aagatgacgc ctatgccgcg
    13921 cccgatctca gccagccgtt cgaaccgcac actgcgcggc ctgacttccg gtgtggcggt
    13981 cgccgcatgc gtctcgacgg aaaccgccgt tgaccgctcc gcaagcgcag cgggcggcgg
    14041 cacacgaccg aaatgtccca cctgacgcac gagcgcaaac acgccgcaca ggatcaacgc
    14101 cgacacaagc gtcgccccgt agagaagtcc tctctgtttt cgtatcgccg ccatgaaatg
    14161 agtcatatcc gccaactccc aataagggaa tagcaagtct agcccgccaa cctgccttcg
    14221 tctgtacatg cgcggacaca cgcaggaaat ggcttgaacg gggcgacaac ggcacaagtg
    14281 acgattatct agcgacagct ttgcccgaca attaaggttt aaccacaagc tgctggttgt
    14341 acactgatca tcgcccgcaa tatagaatgt cgattttgaa ttgtcgggca aagttgtgcc
    14401 cctccgtcgc ttgaccaccg acacgatctg tggcgatcat cgttggcatg aagaaatccc
    14461 tgaagacctc gctgatcgtc atcgcgctgg cgttgctcgc ctgcctcgtc tggctcggat
    14521 tgcgaccgga cgcgccggac gcgccggttg gggcgagcgt cccggaaacg gcacgagggc
    14581 tgtcgttcga ggtgcatgtc gagaagccac gcctcaaccg gttcctcttc ggaatcctcc
    14641 cgaccaaact ggaggataag ctattcggga gcggggaact gcgtttcgac cacgcaagcc
    14701 ccggcgcagg gatcggcagc atcggacaca accgcctcga actccgcgcc gacggctggc
    14761 acctcctcat ggaaaccgac ggcgaaggga aggtcgcacc ggggacgagc ctcgtcttcc
    14821 cgatcctgct cgcagagagg cagcggacgt tgcgctgccg gcccgcagat cgtgccaccg
    14881 ggtatctcga cgccgccacg cgtgcaggct ccgacgtgct cgacggtcgc ttcctcgtcg
    14941 aactcgccac ctgtgagaat gccgagacgg gaaaggtcat cgagtggccg caggcaccgc
    15001 tcaccgtacg cgggagtttt gctggcctgc cacacggcca t
//
LOCUS       DASWNS010000532        12532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_150727, whole genome shotgun sequence.
ACCESSION   DASWNS010000532 DASWNS010000000
VERSION     DASWNS010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12532
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150727"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VGB76_18285"
     CDS             complement(<1..1464)
                     /locus_tag="VGB76_18285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY0100902.1"
                     /translation="MARETNAEVKTEKVSPDLRERARRNPDQRVMVVLQSAGAWSTSH
                     DGLVRAFGANVKRNYQNFDMRAIEVPARVAELLAMQNEFAYVSLDREVRSLGHISLTT
                     GADAARKMTGTSNEYNGTDIGIAVIDSGIYQSHVSFMQASGGGIRIPASVDFTDSGTT
                     ADPYGHGTHVASIAAANPMVAQGAYLGIAPNANILNLRVLDGMGKGSTSALLAALDWV
                     YTNRTAHNIRVVNLSLGTAAVDSYRNDAICKAARKLVDAGIVVVAAAGNEGRDANGTK
                     LYGQIHSPGNEPSVITVGASNSFGTDARTDDVVTTYSSRGPTRSYYTDLAGVRHYDNL
                     VKPDLVAPGNKIIEAQSASNYLVTNNPQLDANVSGSVIKEQMYLSGTSMATPVVSGAA
                     ALLLQANPGLTPNLVKAILQYTAQPLAGYNNLEQGAGQVNLEGAMRLARLVRTDLSAN
                     TLLGAPLLTTAVPTQTSTVARTTFNWGGSVTMKQTFVT"
     gene            complement(1948..3618)
                     /locus_tag="VGB76_18290"
     CDS             complement(1948..3618)
                     /locus_tag="VGB76_18290"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0100903.1"
                     /translation="MFRLVLFVVTVLLVGTPAYAQDKTAEIDKIFSWATTATPGCAVA
                     VSQHGKLIVNRAYGSAELEHDVAFSPGTISEPGSIAKQFTAAAVLLLVEEGRLSLSED
                     IRKYIPELPDYGHKITVDHLLTHTSGIRDWGPLVAQAGAPRRRTVYGNRDALYVILRQ
                     RGLNFAPGEEFSYSNSGYALLVEIIARTSGMSFVEYTRKRLFEPLGMKKTDWRTDYRD
                     IVKNRALAYEKEGDNWKLAMPLENYVGGGGLLSTTGDLLIWNDALTSGRLGAFVTEKL
                     QEPARLNNGRQIKYARGLIVDSYRGVKEVWHSGNAAGYHSWLSRYPAQALSIAVLCNL
                     GSVTATSLAHSVIDQFLPATGAGEAKTPATPAGDTGAAGLDVSGRAGLFVSERNGEPL
                     LLTANNGKLQIVGGDPLVAVTKDRFRNPRGDLFFMSQDEFELNFLSPDEFELKSMEGQ
                     TTRYRRARPYAPTPVELQAFAGRYESDEIGSDYQLTVKENGLVMSLKHSPLGGAEFKP
                     VYRDTFQRGRQTVRFLRDKAGKVLGFDLSNPVIRNLRFTRLRDPNSQH"
     gene            complement(3800..5860)
                     /locus_tag="VGB76_18295"
     CDS             complement(3800..5860)
                     /locus_tag="VGB76_18295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0100904.1"
                     /translation="MIGKPLTFLTPGFLLCALSCVFLFSATALAQNGLPPLIDRELMF
                     GNPEISGAQISPDGKFIAFRKPHKGTVNIWVKRADEPFANARLLTNETRRPPRQFLWS
                     RDGKFILFTKDKEGDENYNLYAVDPGAQPPGGAEVPAARNLTNSEKARTILYNVPRAE
                     PDAVYVGLNDRDPAWHDLYKIKISTGERILVRQNTERLTDWLFDNKDQLRLATRSTKS
                     GDKEVLRVDADGKFTKVYSCSVFETCGLSRFNKDNRRIYMVSNKGEGVDLARLILFDP
                     ETGQEEVVESDPLRRVDLDGASFSTVTGELVFTGYTDDRQRLYFRDKTFEKDYKFLQK
                     KLPNREIWFDSTTNDEMTWLITAASDTEPGEKYLFDRKSKKLELQYRIREKLPREALA
                     ELKSIRYKSSDGLEIPAYLALPRGLAPKNLPTVIIPHGGPWARERWGYDSITQFLANR
                     GYAVLLPNFRGSTGYGKKFLDAGNGQWGERMQDDVTWGVKYLISQGITDPKRVGIMGA
                     SYGGYAALVGVTSTPELYAAAVAINAQNDLISLLASMPATMESERIRVYKRVGDPTTG
                     EGKAQLIRQSPLTHADKIKTPLLVVQGANDVRVKKSQADQIVISLRDRGFPVEYIVAP
                     DEGHGFARRVNIMATMVMAEKFLAKYLGGRYQESLAPEVAKRLQEITVDPKTVKLATN
                     PTGK"
     gene            6169..6945
                     /locus_tag="VGB76_18300"
     CDS             6169..6945
                     /locus_tag="VGB76_18300"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0100905.1"
                     /translation="MRIFAISDLHTDFRENRLLLERLSQEEYTQDVLLVAGDIADRLQ
                     VIGETLTLLRSRFARVFYTPGNHELWTRDEGGDSVEKYFRVMELCRQVGVSTSPGEEG
                     GVRIVPLLSWYETRRGANGRLEGPEEWADFYFCKWPEWIGSPSAYFLKANEPHLKSYD
                     EPVVSLSHFLPRRDLLPPVERLRFKRLPEVAICEPLEEQIRRLRSVVHVFGHSHINCD
                     LRLDGVRYVQHALRYPQERQRQVELVKLVWGEGSGAAARV"
     gene            complement(7151..8389)
                     /locus_tag="VGB76_18305"
     CDS             complement(7151..8389)
                     /locus_tag="VGB76_18305"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY0100906.1"
                     /translation="MTVKNHQFYLNGSPYYYVGANYWYGSLLGLEKDDRKGVGRLRKE
                     LDFLKANGVTNLRLLAGAEGAGMINGVIRVGPPLQPRQGEFDVQVLDGLDLALSEMAK
                     RDMKAVIFLSNNWEWSGGFQQYLDWNGLVPEGMRTRKLTWEEQREIVSKFYGCEPCKA
                     SYNRQASLILGRTNRYNKRKYTEDPTIMAWELANEPRPMRPFAVEAYRRWVADVAALI
                     KARDKNHLVTIGHEGYMGTEDLKLYEEIHADKNVDYLTIHIWPKNWEWFKGHALREGF
                     ANVLEKTLSYIGQHLPVAERLGKPLVIEEFGLPRDGHSFDPAAPTSLRDAYYAKILSF
                     VEQHAAAKGHIAGLNFWAFGGTARPSKGQTFWKAGDSYTGDPPMEEQGLNTVFDSDES
                     TWKVIRATGKSLSGGRAANN"
     gene            complement(8690..9457)
                     /locus_tag="VGB76_18310"
     CDS             complement(8690..9457)
                     /locus_tag="VGB76_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100907.1"
                     /translation="MYVLLLKTLLLTHIIFGGLQGDDKPEAKLVHRFEREIDQYSISQ
                     AVYASLKEYREDSNNIIVLRVCSQDRFPLSLYSSAVIPVVVRGYIQAGWLDPFPQERI
                     PFIRSEACRIEGSTVAPVEVWVVPKGASLPPHVESVQFCQLRFSEFAWNKLKGRPHLS
                     TRREYQMALRKLVTKLRRNPQAVALFWGQYLRKPGSMLEKNLRDLPAFMEQQGFRRDR
                     YLTKFNHFGGDYLEDTPEPQYPDITTVEVSARCRRSR"
     gene            complement(9666..10226)
                     /locus_tag="VGB76_18315"
     CDS             complement(9666..10226)
                     /locus_tag="VGB76_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100908.1"
                     /translation="MAKVQTTVGRWALLAAVGAAWWIEKMVTVEWSYPYCGVRDDGTA
                     AAVYGFPLPYWQPSIVTSLTDFFIPWLYVVNLAVIAGGILLLRPLASRLGASDPRSRK
                     FIFVPAAALLLVTAVAFEVLTLSIGAWRPTSSSSLFSGYTHYSKLRPVAVRVLGRPRD
                     CTASPYWFPDQDARSTGITPSGSARY"
     gene            11247..>12532
                     /locus_tag="VGB76_18320"
     CDS             11247..>12532
                     /locus_tag="VGB76_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100909.1"
                     /translation="MKRILLFAAPLNATFLVLFFCHALLVSAQEEHPHQHEHTEKLGR
                     VSFPVSCSTQAQRQFNRAVAWLHSFEYEEAEKVFNEVTVTDPRCGMGYWGIAMSNYHP
                     IWAPPNAAELKKGWEAVEKARSLGARTARERDYIAAIEFFYKDSGKLDHRTRTLAYSE
                     AMEQLYSRYPTDREAGVFYALTLIARGMMSSDKSYINEKKAAQILNRVLVREPQHPGV
                     AHYLIHSYDYPALAHLALPAARGYARIAPASAHALHMPSHIFTRMGLWQEAIRSNLDA
                     RAAAKAYAVRNRMPGAWDEQLHAMDYLAYAYLQGAQDRQALGVLDELHKIRKVDPPNF
                     KVAYAFTAIPARYALERRQWAEAAKLPLHPGTMETFPWSRFPWAEAHIHFARAIGAAR
                     TGDTAAARQEVERLATIRQSLAEVRGDYDWAKQVEIG"
BASE COUNT         2543 a         3500 c         3707 g         2782 t
ORIGIN      
        1 tgtcacgaat gtctgcttca tggtgacgga gccgccccaa ttgaaggtag ttctggcgac
       61 ggtcgaggtt tgagtaggaa ctgccgtcgt cagcagcggc gcgccgagga gagtgttcgc
      121 ggagaggtct gtgcggacga gtctggcgag gcgcatcgcc ccttcgaggt taacctgacc
      181 cgcgccctgt tcgaggttgt tgtaacccgc gagcggttgg gcggtgtact ggagaatcgc
      241 cttgacgagg ttgggcgtca ggccggggtt agcttgcagc aggagtgccg ccgcgccgga
      301 aaccaccggg gtcgccattg acgtgcccga caggtacatc tgctctttga tcacactccc
      361 gctgacgttg gcgtcgagtt ggggattatt cgtgacgagg tagttgctcg ccgactgtgc
      421 ttcgataatc ttgttgccgg gcgcaacgag gtcgggcttg acgagattgt cgtagtggcg
      481 cacgcccgcg aggtctgtgt agtagctgcg ggtcgggccg cgcgaactgt acgtcgtcac
      541 cacgtcgtcc gtgcgggcat cggtgccgaa gctgttcgac gcgccgaccg tgatgaccga
      601 aggttcgttg ccggggctgt gaatctgtcc gtacagctta gtcccgtttg cgtctctacc
      661 ctcgttaccc gccgctgcga cgaccacgat ccctgcgtcc acgagtttac gcgcggcctt
      721 gcagatggcg tcgttgcggt aggagtctac ggcagcagtg ccgaggctca ggttcacaac
      781 gcggatgttg tgagccgtgc ggttcgtata aacccaatcc agagcggcga gcagcgcaga
      841 ggtgctgccc ttgcccatgc cgtcgagcac acgtagattc aggatgttgg cgtttggcgc
      901 gatgccgagg taagcaccct gtgccaccat cgggttggcg gcggctatag acgcgacgtg
      961 tgtgccatgc ccgtaggggt ctgcggtcgt gccggagtcg gtgaagtcca cgctcgcggg
     1021 gatgcgtatg ccgccgcccg acgcttgcat aaatgagacg tggctctgat agatacctga
     1081 gtcgataacg gcgatgccga tgtctgtgcc gttatattcg tttgaggtgc ctgtcatctt
     1141 gcgtgcggcg tcagcgccgg tggtgagcga gatgtggccg agggatcgaa cctcacgatc
     1201 cagagagacg taggcgaact cattctgcat cgccagcaat tccgcgacgc gggcgggcac
     1261 ttcgatggcg cgcatgtcga agttctgata gttgcgcttg acgttcgccc cgaacgcacg
     1321 cacaaggccg tcgtgtgagg tgctccacgc tccggcagat tgtaagacga ccatgacgcg
     1381 ctgatcggga tttcgcctgg cgcgctccct taggtcggga gagactttct ccgttttaac
     1441 ttcggcgttc gtttccctag ccatgctgct ggcgagaggg ctaaggctca ttagccccgt
     1501 gcacgcggcc aaaaacaagg ccagtgccgc cgcacgcata attagtatac gtcgtttgtt
     1561 tttattcatt ttgtccttcg ggaactccca ctgccgtgct cctgtcgagg agtttaggct
     1621 ggtaagtgcg tgcccgctcc cgttttcaca gagaggaggc tcttgtcggt gacttgatag
     1681 ttaaagagcg tgcccgataa aggtcaggtc ggcaaagtta gagcgagcgg cgtgccagaa
     1741 gccgggaatg tggaatgcgc gaaaacatta gcttttcggc atacacggtg ttagctggag
     1801 gaaaggtggc tgagcagact gactgattcg gcgaatctca atgattgctc aagtcattat
     1861 gaatctgtcg gagagtcatt ttgtgattcg aatggcttcg caggttaatt cgacataagt
     1921 gttagtgagg attaggcaac gcaaatttta atgttgactg ttggggtcgc gcagccgcgt
     1981 gaatctgaga ttgcggatca ccggattgct gaggtcgaag cccaagacct ttccggcctt
     2041 gtcacgcagg aagcgcaccg tctgcctgcc tcgttgaaag gtgtcgcggt agaccggttt
     2101 aaattccgca cccccgagcg gcgaatgttt cagactcatc accagtccgt tttctttgac
     2161 cgtcaactgg tagtcagacc cgatctcgtc gctctcgtat cggcctgcaa aggcttgaag
     2221 ttcgaccggg gtgggcgcat aaggccgggc gcgacgatac cgcgtcgttt gtccctccat
     2281 agacttcaac tcgaactcgt ccggtgacag gaagttcaat tcgaactcat cctgtgacat
     2341 gaagaacagg tcgccgcgcg gattgcggaa acggtctttg gttaccgcca cgagggggtc
     2401 gccgccgacg atttgcagct tgccgttgtt tgcggtcagg agcagcggct cgccgttgcg
     2461 ctcgctaacg aacagccccg ccctgccgct cacgtcaagt cccgccgcgc cggtatcgcc
     2521 agcgggcgtc gcgggcgtct tggcctcccc ggctcctgtg gcgggtagaa actgatcgat
     2581 gacgctgtgc gcgagcgacg ttgccgtcac ggagcctaaa ttgcacagga cggcgatgga
     2641 aagagcctgt gccggataac ggctcagcca tgaatggtag ccagccgcgt tacctgaatg
     2701 ccacacttcc ttgacgccac ggtaggaatc cacgatcagg ccacgtgcat atttgatctg
     2761 cctgccgttg ttcagccttg ccggttcctg aagcttttcg gtgacgaacg cgccgaggcg
     2821 tccgctcgtc agagcatcgt tccagatgag gaggtcgccc gtcgtgctga gcagcccgcc
     2881 gcctccgaca tagttctcaa gcggcatcgc cagcttccag ttgtcgccct ccttctcgta
     2941 agcgagggcg cggttcttga cgatgtcgcg gtaatcggtg cgccagtcgg tctttttcat
     3001 tccgagcggc tcgaacagac gcttgcgcgt gtactcgacg aacgacatcc cgctcgtgcg
     3061 cgcgatgatc tcaaccagca gtgcatagcc gctgttcgaa taggagaatt cttcgccggg
     3121 cgcgaaattg aggccgcgct ggcgcaggat gacgtacaac gcgtctctat tcccgtagac
     3181 ggttctcctg cgcggcgcgc ctgcctgcgc aacgagcggt ccccagtcgc gaatgccgct
     3241 ggtatgtgtg agcagatgat ccacagtgat cttgtggccg tagtcgggca gttcgggaat
     3301 gtacttgcgt atgtcttcgg aaagggaaag gcgaccctct tcgacgagaa ggagaactgc
     3361 cgcagcggta aactgcttcg caatcgatcc gggttcggag atcgtgcccg gactgaacgc
     3421 gacgtcgtgt tcgagttcag ctgaaccgta agcgcgattc acgatcagct ttccatgttg
     3481 cgagacggcg accgcgcatc cgggcgtagc cgtcgtagcc caactgaata tcttatcgat
     3541 ctcggcggtc ttgtcctgcg cgtaagcagg cgtgccgaca agcaagactg tgaccacgaa
     3601 caatactagt ctgaacattc aatcctcttt agttgaagcg cggggaaatt tcaggccggc
     3661 tgtgggggat ttaccggaga cggttgactg tagggtagct cgatacggtt cctcgcttcg
     3721 agcgcatgtc gcggctggct ccgcgcttat cgcggagcca gccagagcta ccgactgctt
     3781 cggtgcgggg gggaggccgc tatttgccgg tcgggttggt ggctaatttg acggtcttgg
     3841 ggtcaaccgt tatctcctgc aaacgtttgg ctacttcggg cgcgagcgat tcctggtagc
     3901 gtccgccgag gtatttagcc aggaacttct cggccattac catcgtcgcc atgatgttga
     3961 cgcggcgcgc gaagccgtgg ccttcgtcgg gtgcgacgat gtactcgacc gggaagcccc
     4021 tgtcgcgcag ggagatcacg atctggtcgg cctggctttt cttcacacga acgtcgtttg
     4081 ctccctgcac gacgagcagc ggggtcttaa tcttgtcagc gtgcgtcagc ggcgattgtc
     4141 gtatgagctg cgcttttcct tccccggtgg tggggtcgcc cactcgtttg tataccctga
     4201 tgcgctctga ctccatggtc gccggcatgg atgcgaggag cgaaatcaga tcgttctggg
     4261 cattaatcgc caccgccgcc gcgtagagtt ccggcgtgga ggtgacaccc accagcgcgg
     4321 catagccgcc gtaagaagcc cccataatgc cgacgcgctt cggatcggtg atgccctggc
     4381 taatcagata cttcacgccc caggtcacgt cgtcctgcat ccgctcgccc cactgcccgt
     4441 tgcctgcatc cagaaacttc ttgccgtagc ccgtcgagcc gcgaaagttc ggcagcagaa
     4501 cggcgtagcc acggttagcc aggaactgtg ttatggagtc gtagccccaa cgttcgcgcg
     4561 cccatggtcc tccgtgtgga atgataacgg tcggaaggtt cttcggcgca agacctctcg
     4621 gcagggctaa gtaagcggga atctccagcc cgtcggacga tttgtagcga atcgatttta
     4681 gttcggcgag agcttcgcgc gggagctttt cgcggatacg gtattgcaac tccaacttct
     4741 tcgacttgcg gtcgaagaga tacttctcgc cgggttccgt gtcactggca gccgtgatga
     4801 gccaggtcat ctcgtcattg gtggtcgagt caaaccagat ctccctgttc ggcaacttct
     4861 tctgtaaaaa cttgtaatct ttctcgaatg tcttgtccct gaaatagagc cgctggcggt
     4921 cgtccgtgta gcctgtgaag accaactcgc cggtcactgt cgagaaggat gcgccgtcca
     4981 ggtctacacg gcgcagggga tcggattcga cgacctcctc ttggcctgtt tcggggtcga
     5041 acaagatgag ccgcgccagg tctacgccct cgcccttgtt ggaaaccatg tagatgcggc
     5101 gattgtcttt gttgaagcgg ctcaggccgc aagtctcgaa gacgctgcac gaatagactt
     5161 tcgtaaactt gccgtcggcg tccactctga gcacttcctt gtcgccgctc ttggtcgagc
     5221 gcgtggcgag acgcaactgg tctttgttgt cgaagagcca gtccgtcagc cgttccgtgt
     5281 tctgtcgaac gagtatgcgc tcgccggtcg agattttgat cttatacagg tcgtgccagg
     5341 ccgggtcgcg gtcattcagg ccgacgtaga cggcgtccgg ctcggcgcgc ggcacgttgt
     5401 aaagtatggt cctggccttc tccgaattgg tgagatttcg cgcggcggga acttccgctc
     5461 cgcccggagg ctgcgcgccg ggatcgacgg cgtagaggtt gtaattctcg tcgccctctt
     5521 tatccttcgt gaacaggatg aactttccgt cgcgactcca taaaaattgt ctgggcgggc
     5581 gtctagtctc attggtcagc aggcgcgcgt tcgcgaacgg ctcgtccgcg cgtttgaccc
     5641 aaatgttcac cgtgcccttg tggggcttgc ggaaggcgat gaacttgccg tcgggagaga
     5701 tctgcgcccc ggagatttcc gggttgccga acatcagttc acggtcaatc aacggcggca
     5761 gaccgttctg cgccagcgcg gtcgcgctga acagaaaaac gcatgagagc gcgcagagta
     5821 aaaatccggg cgtcaaaaat gtaaggggtt ttccaatcat atctctaaaa gctcctgata
     5881 gatgtctgtc gtgagtgctg gctgctccgg cgataaacgg ctccaaaaag ataaacgtgc
     5941 gggaggacga aatgttaacg tcaggcgtta atacttttta tcaggagcga cgaatttaag
     6001 tccggttctg tgcaagcgtt gccgcccggt tataatctgc gccttgagcg gaagtgaggc
     6061 tacgggtcgc gcgaagttca tctcgaaaat ctttcaggtt gcgacatagt ggcatagact
     6121 caaccaactt tcgcgcctaa aataatctat gcgacacagc tagtccctat gcgaattttt
     6181 gccatctccg atcttcatac agactttaga gaaaaccggc tgctccttga gcgtttgtcg
     6241 caggaagagt acacgcagga cgttctgctc gtggccggag acattgcgga tcgtctccaa
     6301 gtcataggcg agacgctgac gctcctgcgc tcaagattcg cgcgcgtctt ttacacgccg
     6361 ggcaatcatg agttatggac acgggatgaa gggggcgact cggttgagaa gtacttccgt
     6421 gtgatggaac tgtgccgtca ggtcggcgtc tcaacctcgc cgggggagga agggggagtg
     6481 cggatcgtgc cgctgctctc ctggtatgag acgaggcgag gcgcgaacgg caggctggag
     6541 ggaccggaag agtgggcgga tttctatttc tgtaagtggc cggagtggat cggctcaccg
     6601 tccgcctact tcctcaaagc gaatgagccg cacctcaaaa gttacgacga gccggtcgtc
     6661 tcgctctcac attttctgcc ccggcgcgac ctcctgccac ccgtcgaaag gctccgcttt
     6721 aaacgtctgc cggaggtggc gatctgcgag ccgctcgaag agcagatcag gcggctgaga
     6781 tcagtcgtcc acgtgttcgg gcacagccac attaactgcg acctgcggct cgacggcgtt
     6841 agatacgtcc agcacgcttt gcggtatccg caagagcgtc aacggcaggt tgagcttgtg
     6901 aagttggtgt ggggggaggg cagcggcgcg gcggcgcgag tctgaaaggc gcggccagca
     6961 aaagcgcgca gatgttcgca gccgtgcgtc gtgctcaagc ggagattact cattacggct
     7021 cttaactcgt ttgcgtccga tcccggacgg caatctgcaa gggtggttga aaaatttccc
     7081 aaccagacaa aatattgcgg cggcatcctc tccagcccaa acagatgcgc cgctcccgtt
     7141 cagccgcctg ctaattatta gcagccctgc ctccactcag gcttttgccg gtagccctga
     7201 tcaccttcca tgtgctctcg tcgctgtcga agacggtgtt gagtccctgc tcttccatcg
     7261 gcgggtcgcc cgtgtagctg tcgcccgcct tccagaacgt ctgtcccttc gacggccttg
     7321 ccgtaccccc gaaagcccag aagttcagac cggcgatgtg ccccttagcc gcagcgtgtt
     7381 gttcgacgaa cgataatatc tttgcgtagt aggcgtctcg cagggaggtg ggggctgcgg
     7441 ggtcgaacga gtggccgtcc cgtgggaggc cgaactcttc gatgacgagg ggcttgccca
     7501 gcctctcggc caccggcagg tgttggccga tgtagctcag agtcttttcc agcacgttag
     7561 cgaaaccttc tcttagcgcg tgacccttga accattccca gttcttcggc cagatgtgta
     7621 tcgtcaggta gtcaacattc ttgtcggcgt gaatctcttc gtagagtttc aggtcttcgg
     7681 tgcccatgta gccttcgtgg ccgatggtaa cgaggtggtt cttgtcccgc gctttgatca
     7741 aggcggccac gtcggccacc cagcgacggt atgcctcgac cgcgaatggc cgcatggggc
     7801 gcggctcgtt cgccagttcc cacgccatga tcgtggggtc ttcagtgtac ttccttttgt
     7861 tgtacctgtt ggtcctgccc agaatcaggc tggcctgccg gttgtaactg gccttgcagg
     7921 gttcgcaacc gtagaacttt gaaacgatct ctcgctgctc ctcccaggtt aatttcctcg
     7981 tcctcatccc ctccggaacg aggccgttcc agtcgaggta ctgctggaag ccgccgctcc
     8041 actcccagtt gttgctcagg aagatgaccg ccttcatgtc gcgcttcgcc atctcggaga
     8101 gcgccagatc gagtccgtct aaaacctgca cgtcgaactc gccctgacgc ggctgaagcg
     8161 gcggccctac cctgatcacg ccgttaatca tcccggcgcc ctccgcaccg gccagcagcc
     8221 ggaggttagt gaccccgttt gccttgagaa aatccaactc cttacgcaat cgcccgacgc
     8281 ccttccggtc atccttttcg agtcccagaa ggctgccgta ccagtagtta gccccgacgt
     8341 agtaataggg gctgccgttc aggtagaact ggtggttctt cacggtcacg aatgaagacc
     8401 gcgtctgtgc cgtcgcagta aatgcctgtg ccgtgacgag cgtactcaac aaggctaacg
     8461 ccgcaaggac tatccggttc actgttgaac tcctgaacaa tttgtaaacg gtcgcatgct
     8521 atgaacgaat ccctcaacct cttcacacaa gagttctctc gcaaacatcc tcgccctccg
     8581 ctatgtaagc cacctgacgc ccggcaacac gcgcccctgt tcgactatta tgagaggcag
     8641 cgtggcggcg gcacctgctg ttgttagacc gcgcgggcag ggaacagact caacgtgatc
     8701 tcctgcacct tgctgatact tccaccgtgg ttatatcagg atactgtggc tcaggtgtgt
     8761 cttccagata atctccacca aaatgattaa acttcgtgag ataacggtcg cggcggaagc
     8821 cctgttgctc catgaaggct ggtagatcac gcagattctt ctccaacata gaacccggct
     8881 ttcgaaggta ctgcccccag aatagcgcga ctgcctgcgg attcctgcgt aacttagtga
     8941 cgagctttcg caaagccatt tgatattctc ggcgcgtcga gagatgtggg cgacctttta
     9001 gcttgttcca cgcaaattcg ctaaagcgca actggcagaa ttgcacggat tcaacatgtg
     9061 gcggcaaaga agccccctta ggaaccaccc acacctcaac cggagctact gtggaaccct
     9121 caatcctgca agcctctgaa cgtataaacg gtattcgctc ttgtggaaat ggatctaacc
     9181 acccggcttg tatgtatccc cgaacgacca cagggatcac cgcagaagaa tagagagata
     9241 aaggaaatct gtcctgcgag caaacccgca ggacgataat attatttgag tcttcgcgat
     9301 actcctttaa tgaagcatag acggcctgcg atattgagta ttgatctatc tccctttcaa
     9361 agcggtgaac gagtttagct tcaggcttat catcaccctg caagccgcca aagataatgt
     9421 gcgtgagaag taaggtttta agaagaagaa cgtacatgtt aacaaccttt gactatcatc
     9481 ccgagagcgt aggtgctaac gctcgaacca acccgcgccg agcgacagcg acaatttaca
     9541 tcgaaatctt atctggagag ctttcgtcta acgatatatt gaagcgcagc tttatgtcac
     9601 ttgcggcaac tccgcgcttt ccgacaagct ccattatgtc agagagacgc cgcacgtcga
     9661 tgagttcaat atcttgcgga accggatggt gtgatgccgg tgcttcgggc atcttgatcc
     9721 gggaaccagt agggcgaggc cgtgcagtct cgtggccgtc cgagaacacg aaccgcgaca
     9781 ggccgcagtt tgctgtagtg cgtgtatccc gagaataatg aagacgaaga ggtgggacgc
     9841 caggcgccga tggagagcgt cagcacttcg aaagcgactg ccgttacaag cagaagggca
     9901 gcggcgggaa cgaagatgaa cttgcgggag cgcgggtctg atgcgcctaa gcgagacgcc
     9961 agagggcgca gcagcaggat gccgcccgcg atcaccgcga gattgaccac gtacagccac
    10021 gggataaaga agtctgtgag agaagtgacg atgctgggct gccagtacgg gagcgggaac
    10081 ccgtagactg ccgcagccgt gccgtcgtcg cggacgccgc agtatgggta agaccactcg
    10141 accgtcacca tcttctcgat ccaccaggcc gcgcccaccg cagccagcaa tgcccaacgt
    10201 cctacggtcg tctgtacttt tgccatgatc tctcaaagcc tgacaactgg tgaggcagcg
    10261 gcgcgaccta tcaactgata ctttgattct acgaaaactt tagaattata tctggtgttt
    10321 cgggttttta ccgagcatcc gacgtaccac tgtccgttag ggttgtagag atcaatctcc
    10381 atccccggct tatttccata aaaagtggac gcacctacag aacggatacg atgaactatg
    10441 acaccactca tgtcatagcc gcccggcgag gatgtcggag cgcgacgctc ttcctgctcg
    10501 gtgtgcggca agcctgcacg cccctgctcg gcggaagatg tctctatgct ttcgcgtggg
    10561 ggtgagagcg aattgtaccg caccatgcgg cctgacttac ttaggataaa agcccgaatg
    10621 aatcgcccga gtccgataac cccatgattt tacggcactc cgcaccgtta gactgccacg
    10681 gagtgctttt gcacaacagg ggcgacttag tttggccgga gtaaattttt tcgatcagac
    10741 ttttattgca aagcctactg cgacctggtc tgacgagaca cttatgttcg gctctgattt
    10801 tacataattt ggtttatcgg actcagaagt ggcgtgatag agctattatg aaaagtgaca
    10861 gttgacgggt ggagtcagga agctgtcaac atgatgtggg gagggcgggt caaaagattg
    10921 aggggttaat gactcacgga atcaggcgac atgagcactc agaagccgat ggttatacaa
    10981 agctgcgaag tcatgggtga aagaattaat agcggagcca atttcttact ttactatctt
    11041 gataataata tgcggcggct tgggtatttt tttctttctg gtgcatataa tgggagaggg
    11101 tcgaaaggat gaagagactg ctaagcccaa ctaggccaat cttccgcgcg aagattcagc
    11161 acgagaatgg ttcctcgcct ccaagacctt cgttgaggag caccaacggt tcgtgatctt
    11221 tgaagaccca agaaggagga gaatctgtga aacgcatact tctgttcgcg gcaccgttaa
    11281 acgcaacttt cctcgtcctc tttttctgtc acgctctctt agtcagtgcg caggaagagc
    11341 acccgcatca gcacgagcat acagagaagc tcggacgggt ttccttcccc gtctcctgtt
    11401 ccacccaggc tcaaaggcag ttcaatcgcg cagtggcatg gctccattct ttcgagtacg
    11461 aagaggctga aaaggtcttc aacgaagtta ccgtcacaga cccgcggtgt ggcatgggtt
    11521 actggggcat cgcaatgagc aactaccatc ccatctgggc gccgccgaac gcggcagagt
    11581 tgaagaaggg atgggaagcc gtagaaaagg ccagatccct cggcgcgcgg actgcgcgcg
    11641 agcgagatta catcgcggcg atagagtttt tttacaaaga ctccggcaag ctcgatcacc
    11701 ggacgcgcac gctcgcctac agcgaggcga tggagcaact ttacagtcgc tatccgacag
    11761 accgtgaagc gggcgtcttc tacgcgctga cgctcattgc aagggggatg atgtcgagcg
    11821 acaagagtta tatcaacgaa aagaaggccg ctcagatttt gaaccgcgtt ctcgtgcgcg
    11881 agccgcagca tcccggtgtg gcacactacc tgattcacag ttacgactat ccggcgttgg
    11941 ctcacctggc gctgcccgcc gctcgtggtt atgcaaggat cgcgcccgcg tcggcacacg
    12001 cactgcacat gccttcacac atcttcacgc ggatgggttt gtggcaggaa gcgattcgtt
    12061 cgaacctcga cgcgagggcg gcggcgaagg cttacgccgt cagaaatcgt atgcccggcg
    12121 cgtgggatga gcagcttcac gcgatggact atctggccta tgcctacctg caaggcgcgc
    12181 aggatagaca ggctcttggc gtgctcgatg agttgcacaa gattcggaaa gttgacccgc
    12241 cgaatttcaa agtggcctat gccttcacag ccatccccgc gcggtatgcg ctcgaacgca
    12301 ggcagtgggc ggaggcggcc aaattgcctc tgcatccggg tactatggag acgtttccct
    12361 ggtcgcgctt cccctgggct gaagcccaca tccatttcgc gcgcgcaata ggggctgcgc
    12421 gtacgggcga tacggctgcg gcgcgtcagg aggtcgagag gttggcgacg atccggcagt
    12481 cgctcgccga agtcagaggc gattacgatt gggcaaaaca ggtggagatc gg
//
LOCUS       DASWNS010000533         8830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_46088, whole genome shotgun sequence.
ACCESSION   DASWNS010000533 DASWNS010000000
VERSION     DASWNS010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46088"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VGB76_18325"
     CDS             complement(<1..417)
                     /locus_tag="VGB76_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY0100910.1"
                     /translation="MSFQLRSEFQPQGDQPQAIDALVRGLDEGQKHQVLLGITGSGKT
                     FSVATVIQRTQRPTLVIAHNKTLAAQLYQEFKTFFPENAVEYFVSYYDYYQPEAYVPA
                     ADMYIEKEATINEEIDRLRLSATRALFERRDVIVVAS"
     gene            complement(558..1196)
                     /locus_tag="VGB76_18330"
     CDS             complement(558..1196)
                     /locus_tag="VGB76_18330"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEY0100911.1"
                     /translation="MSNAGAQGTYLTGIDVSHYQGAVNWAEVRGAGCAFAFAKATESN
                     AIVDPEFAANWNGMKAAGIVRGAYHFFRPEQNAVAQANHFLQTVQLEPGDLPPVIDIE
                     LNDGVTGGALIAGVGNWIDTVAQATGMTPMIYTAAYFWNEYMNDGFGKFPLWLAHYTN
                     APTPQPLPHGWTNWTFWQYSQSLPIPGVSGNADHNRFNGAQSDLEALTKQAT"
     gene            complement(1372..5022)
                     /locus_tag="VGB76_18335"
     CDS             complement(1372..5022)
                     /locus_tag="VGB76_18335"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0100912.1"
                     /translation="MRQNKQSDSLFCPRRFVLLLVAVASFLCLAVVNSERTRAQQPNA
                     NSATQKPRTKRLTPLRTSDTPDGSRATITSDGELNDYSAYRSGDRFIVVVPQAEGAGG
                     GGARGRGFEGAQVERRGKDVVYTFKLQPGATARVNQRFNRLDVQFSAPKGAGGNTANA
                     NARPTPAPTPRRPLPEEAGITNPTANANSARPTPEVRRTPDANANSSGVLTVDTQPTP
                     LNAASPNASPLSGVVPSPTLAPTVAPSPGMEQIAQVQPTIAPPVSITTNTTPANAPTS
                     VGAVVLRNWPWLIAALLAVGIGLLFVSRFSSNRDQSRGLPPATPTGTKPSAVKETKPA
                     ELKPTSAATAAATIGATNSAANAPATGVTGTNAAGTSAAGSAAIAAGTAATAANLATS
                     GKQSKKKKKKTEKAKRQLAANELKAGGADAGTGAADAATTTTDSGVGAGVIASIVVAG
                     AAGAAGGASAVEESNAAGTKAGESKAIEAVAPQTQATETPAVAFDPERIEVEIKKLLA
                     GESYDESLINTTDAGTRQLVSAELMQAVAGRNAERKAHARTAFLKHGYFEEATRALQS
                     AEAPGERASAARTLGLYGDRSATPHLTAALEDPAADVRRTTVEALAELADPAAVGALE
                     ALRWRETSRQVPRALIQRAIEASKAAEEEETAATAAEADDDTPTLVTPTPAEAATALA
                     ATETPELETKEIELKEAEAGVAATAVVVAEEPDAQTADSPVLTVSVTETPETAVAVEE
                     APVVAIEAAPTVETSDAIDEAKIDEAEVEAFETKALELASPQVEAAPLEEVTAEFETT
                     AATPAETDLAATLAAKEASLIKAREQQAEAQTADSFVAASTETSFDTSADMAAPVNPE
                     AASAETVTTKADGETSPVYHSDFAVGSGKEPAAARNVWSDADADEHEVTIQSIEPLDA
                     SAAPPAEPLGTFYAEGLHAAPEAGRGEESASLFDSLEVETFETNEITVAEPTPVTAKP
                     VDFELVEDTPTQPLTRERRPSFDEDSLRGTEVVKADKGLSLADGSDEESYSIIPKGIQ
                     VRLASEDASERAASVLALSRLNTNEAFNEICVAFDDPAEEVRNAAARALYDLNEDRAD
                     SFTRALRESPLERRRQIGAALAGSGLADEAVSNLTGESRDKTYDAFSLLFLMAKAGEV
                     VPLIRAIESHHDNEVRLAVVKLLALSGQHDILPAFRRLAVRGSLPTEVRSAVMEAIYQ
                     ISSQPQNATPAT"
     gene            complement(5107..7593)
                     /locus_tag="VGB76_18340"
     CDS             complement(5107..7593)
                     /locus_tag="VGB76_18340"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0100913.1"
                     /translation="MRHLSHKISRRARIGVSCVSLGLAVTIFPLVVPQAQRAGKESGV
                     NVMGVTTRTNGSDTVVSIAADSPLSRAQTWQDSEGFHVVVYKGRGNPGGVPRGVKVRR
                     VGESLEFVVPVKPGGSVYVQPRFNSLDLVVGGGLHTAAETARENSPAQSTRTATAREA
                     QQAASKLRANGGNGATSGVQARESVVALKNDRAKPNLTPLLAPPAALKFSTAPNTTQP
                     ATAASNGTNNGVNAVSPAAQNAPANGQAAHETQIVNPASAEPPATSTQNASPAAMQVQ
                     VAQPAGGSSMKRLLVVLSVCFSTGFAAFMFIFLRSRRKTATSNPAKGESRKTTEAAST
                     VVEFEGEAQLLEQRKGERRTSARRTEDKQALVPTGGGAAVTASQSLEKSERKSSTPAH
                     MNVPAVIFGAYQIDQEVEKLVAGEPHRIEVLASRATDDRRAIEASLLKVLNSHSLGQT
                     ERERARFALEEYGFVARHSASVLLAPDAYERSSAARALGEMRSRASLPFLLEALYDGE
                     QIVCTEAVKSLGALGLPSAIGALLDMARRHPNVPPALLGHALSACSVDCLSLEASADG
                     DSFALPEPAIDPFTGEIRGFEPVAAFEQLPEWMEDETLADALERLDSADVEARVSAAQ
                     QLAQFQVQRSVAALTSLAANDRDAAVRAAAVTSLGNIGHETVFPSVLISMADEAREVR
                     AAAARALSRLSFDRADAYVRVSETADEVTLRHLASACVGAGLANQALSRLASDDRRQA
                     YEAFSLLSLVAKGGQADLILRVVQEHKDLNVRLAASRLLGLMGDAELDTSLRRIALGA
                     NAPEKLRTAILEAIYRGEPQGVAGGVGEAN"
     gene            complement(7845..8624)
                     /locus_tag="VGB76_18345"
     CDS             complement(7845..8624)
                     /locus_tag="VGB76_18345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0100914.1"
                     /translation="MSTKPAANNPRAKDAEGEEPEWVDRKMTLLVIEDDENISTAISE
                     YFSKTGYNVRSAADGVAGVESALQDHPDAIILDLMLPKMDGLAVCRELREKAPSIPIL
                     MLTAKDDVVDKVLGLEMGADDYITKPFNLRELDARIKSVLRRVKVASRSQSGGDEAPI
                     LRGKLRIDPAKREVTVGDRPVELTPKEFELLRLFASNPGRVFPRKYLLEKIWDYSYEG
                     YDRTIDSHINRLRAKIEDNPENPQMVLTVWGIGYKFSDEQR"
BASE COUNT         1382 a         2708 c         2934 g         1806 t
ORIGIN      
        1 ggaggcgacg acgatcacgt cgcgccgttc aaacagagcg cgcgtggccg agaggcgcag
       61 gcgatcgatc tcctcgttga tggtcgcctc tttttcgatg tacatgtcgg cggcgggaac
      121 ataagcctcc ggctggtagt agtcgtaata agagacgaaa tattcgacgg cgttttcggg
      181 aaagaaggtt ttgaactcct gatacaactg cgcggcgagc gtcttgttgt gtgcgatgac
      241 gagcgtgggg cgttgcgtgc gctggatgac ggtggcgacg gagaaggtct tgccgctgcc
      301 cgtgatgccg agcaacacct gatgcttctg cccttcgtcg aggccgcgca cgagcgcgtc
      361 gatggcctgc ggttggtcgc cctgcggttg aaattcggaa cgaagctgaa aagacatgcg
      421 gatagaataa cccgacgcgg gcgcaggctc aaaaagggat gggggaatgc aggatgaggg
      481 atgaatcagc agcactcact ccattgaatg ctttttctga ttcatcccgc atccctccgc
      541 cctcatccct ttctgcccta tgtcgcctgc ttggtgagag cctcaaggtc ggattgtgcg
      601 ccgttgaagc ggttgtggtc ggcgttgccg gagacgccgg ggatggggag gctttgcgag
      661 tattgccaga aggtccagtt agtccagccg tgcgggagcg gttggggcgt gggcgcgttg
      721 gtatagtgcg cgagccagag cgggaacttg ccgaagccgt cattcatgta ctcgttccag
      781 aagtaagcgg cggtgtagat catgggcgtc attccggtcg cttgcgctac cgtgtcgatc
      841 cagttgccga cgcccgcgat gagcgcgccg cccgtgacgc cgtcgttcaa ttcgatgtct
      901 atgacgggcg ggaggtcgcc gggttcgagt tggacggttt gcagaaagtg gttggcctgc
      961 gcgacagcgt tctgctcggg gcggaagaag tggtacgcgc cgcgcacgat tcccgccgct
     1021 ttcatgccgt tccagttggc cgcaaattcc ggatcgacga ttgcgttgga ttcggtcgct
     1081 ttggcgaacg cgaaggcgca gcccgcgccc cgcacttcag cccagttgac cgcaccctga
     1141 taatgcgaaa cgtcgatgcc ggttaaatac gtgccttgtg ctccagcgtt actcataagt
     1201 ctgctttctc ctctttatcg tcgcgtcagg cggcgcgttt ttcagtgggg atgcggaaac
     1261 gcgtgtgtcg cactcgcgtc aaaagttaaa cgggcggtcg gcgcgtggtg tagcgcgtct
     1321 cccgcccgtt cttatgttcg ctcgacggaa caaaaattgt ctcaatccgg ctcaggtcgc
     1381 aggggtggcg ttctgcggtt gactgctgat ctggtagatg gcctccatga cggcggagcg
     1441 cacttcggtc gggagcgaac cgcgcacggc gaggcgacgg aaggccggaa gaatgtcgtg
     1501 ctgccctgaa agcgcgagca gtttgacgac ggcgaggcgc acctcgttat cgtggtggct
     1561 ctcgatggcg cgaatcagcg gcaccacttc tccggccttc gccatcagga agagcagcga
     1621 aaaggcgtcg taggtcttat cgcggctctc gcccgtgaga ttgctgactg cttcgtccgc
     1681 cagacccgaa ccggcgagcg ccgcgcctat ctggcgacgc cgttcgagcg gcgactcgcg
     1741 cagggcgcgc gtgaaggaat cggcgcggtc ttcgttgaga tcgtagagcg cgcgggcggc
     1801 ggcgttgcgc acttcttccg caggatcgtc gaaggcgacg cagatttcgt tgaaggcttc
     1861 gttcgtgttg aggcgcgaca gcgcgaggac gctcgcggcg cgttcgctcg catcctcgct
     1921 cgcaagccgc acctgtatgc ccttcggaat gatcgaataa ctttcttcat ccgagccgtc
     1981 ggccagactc aagcccttgt cggctttcac cacctccgtg ccacgcaagg aatcttcatc
     2041 gaaggacgga cgccgctcac gcgtgagcgg ttgggtcggc gtatcttcga cgagttcgaa
     2101 atcaaccggc ttcgcagtta caggcgtggg ttcggcgaca gtgatctcgt tagtttcaaa
     2161 cgtctcgact tccagtgagt cgaacagcga ggccgattct tccccgcgcc ccgcctccgg
     2221 agcggcgtgc aagccttcgg cgtagaaggt gccgagcggt tcggctggcg gcgcggcaga
     2281 agcgtcgaga ggctcgatgc tttgaatcgt gacctcatgc tcgtctgcgt ccgcatcgct
     2341 ccagacgttg cgggcggcgg caggttcctt gccggagccg actgcgaaat cggagtggta
     2401 gacgggcgac gtttcgccgt cggctttcgt cgtgacggtt tccgcgctcg ccgcttccgg
     2461 gttgacgggc gcggccatgt cggcggacgt gtcgaacgaa gtctccgtag acgccgcgac
     2521 gaaagagtcg gcagtttgcg cttccgcctg ctgctcgcgc gctttgatga ggctcgcttc
     2581 tttggccgcg agcgtcgccg cgagatcagt ttcggcgggg gtggccgccg tcgtttcaaa
     2641 ctctgctgtg acctcttcca ggggcgcggc ttcgacctgc ggcgaggcca actccaaagc
     2701 cttcgtctca aacgcttcaa cttccgcttc atcaattttc gcttcgtcaa tcgcgtccga
     2761 cgtttcgacg gttggcgcgg cttcgatagc gacgaccgga gcctcttcga cggcgacagc
     2821 cgtttccggc gtttccgtca cggagacggt taagacgggc gagtcggcgg tctgcgcgtc
     2881 gggttcttcg gcaacgacca cagccgtcgc ggcgactccg gcttccgcct ctttcaactc
     2941 gatctctttc gtttcgagtt ccggcgtttc cgtcgcggcc aaagctgtcg cggcttcggc
     3001 gggtgtcggc gtaacgagcg tcggcgtgtc gtcgtccgct tcggctgcgg tggcggctgt
     3061 ctcttcttcc tcagcggcct tgctcgcctc gatggcgcgc tggatgagcg cgcgcggcac
     3121 ctgtctggac gtttcgcgcc agcgcagcgc ttcgagcgcg ccgacggctg ccgggtctgc
     3181 gagttccgct aaggcttcga ccgtcgtacg gcgcacatcc gccgcagggt cttcgagcgc
     3241 ggcggtaaga tgcggggtcg cgctgcggtc gccgtagagt ccgagcgtgc gcgcggctga
     3301 ggcgcgttcg ccgggggctt cggcggactg tagtgcgcgc gtggcctctt caaaatagcc
     3361 gtgcttgagg aaagccgtgc gggcgtgcgc tttgcgctcg gcgttgcgcc cggcgacggc
     3421 ctgcatcaat tcggctgaga ctaactgacg cgtccccgca tccgtcgtgt tgatcaaact
     3481 ctcgtcgtag ctttcgcctg cgagcaactt tttgatctcg acctcgatgc gttcggggtc
     3541 gaaggcgacc gccggagtct ccgtggcctg cgtctgtggt gcgaccgctt cgatggcttt
     3601 cgactcgccc gctttcgtcc cggcagcgtt gctctcttca actgcggacg cgccgcctgc
     3661 cgcgcccgcc gcaccggcga ccacgatgga agctatgacc cctgcgccca cgccggagtc
     3721 cgtcgtggtg gttgcggcgt ctgccgcgcc cgttccggca tccgcgccgc ccgccttgag
     3781 ttcgttggcg gccagttggc gtttcgcctt ctccgtcttc ttcttctttt tcttcgactg
     3841 tttgccggag gtcgcgaggt tggcggcggt ggcggcggtt cccgctgcga tggctgccga
     3901 ccctgcggca gacgttccgg cggcgttcgt tccggtcacg ccggtcgccg gggcgttggc
     3961 ggcggagttg gtcgcgccga tagttgcagc ggcggtcgcg gcggaagtcg gtttcagttc
     4021 ggcgggcttc gtctctttaa ctgcggaagg ctttgtgccg gtgggcgtgg cgggcggaag
     4081 ccctctgctc tgatcgcggt tgctgctgaa acgtgagacg aagaggaggc cgatgccgac
     4141 cgccagaagc gcggcgatga gccaaggcca gttgcgcagg acgaccgcgc cgaccgaggt
     4201 tggagcgttg gccggagtag tgttcgtcgt gatcgagacg ggcggggcaa tcgtcggctg
     4261 cacctgcgcg atctgctcca tgccgggcga cggcgcgacg gtcggcgcga gcgtggggct
     4321 ggggacgacg ccggagagcg gcgaggcgtt cggcgacgct gcgttgagcg gcgtcggttg
     4381 cgtgtcaacc gtcaggacgc cgctgctgtt ggcgtttgcg tcgggcgtgc ggcgcacctc
     4441 cggcgtcggg cgcgcgctgt tagcgttcgc cgtaggattc gtgattccgg cctcttcggg
     4501 cagtgggcgt ctcggagtcg gcgcgggcgt cggcctcgcg ttggcgttgg cggtgtttcc
     4561 ccccgcccct ttcggcgcgc tgaactgcac gtctagacga ttgaaacgtt gattgacacg
     4621 cgcggttgcg ccgggttgga gtttgaaggt gtagacgaca tctttgccgc gccgttcgac
     4681 ctgtgcgcct tcaaaaccac gaccgcgcgc gccgccgccg cccgcgcctt ccgcctgcgg
     4741 cacgacgacg atgaagcggt cgccgctgcg ataagccgag tagtcgttca gttcgccgtc
     4801 ggaagtgatc gtggcgcgcg aaccgtcggg cgtgtcgctc gtgcgcaggg gcgtaaggcg
     4861 tttggtgcgc ggcttttgcg tcgcgctgtt ggcgttgggt tgctgcgcac gcgtgcgttc
     4921 ggagttgacg acggcgaggc agagaaaact ggcgacggcg acgagcagga gcacgaaacg
     4981 ccgggggcaa aaaagcgagt ccgattgttt gttttgacgc attgcttatg gcactcaggg
     5041 cagacaggag cgtgtcaggg ctacgtaacg cggcattatg ctataggcgg cgcgctcttg
     5101 tcatcatcaa ttcgcttcgc cgacgcctcc ggccacgccc tgcggttcgc cccgatagat
     5161 ggcttcgaga atggccgtgc gcaacttctc cggcgcgttt gcgccgagcg cgatgcggcg
     5221 caaggaggtg tcgagttcgg cgtcgcccat cagtccgagc aggcgcgagg ccgcgagcct
     5281 gacgttcaaa tccttgtgct cttgcaccac tctcaagatc agatccgcct gcccgccctt
     5341 cgccaccagc gacaacagag agaaggcttc gtaagcctga cgccggtcat cgctcgccag
     5401 acggctcaaa gcctgattgg caagccccgc gcccacacac gccgacgcca gatgcctgag
     5461 cgtcacttcg tctgcggttt ccgacacgcg cacgtaagcg tctgcgcggt cgaagctgag
     5521 acgcgagagc gcgcgcgccg ccgccgcacg aacttcgcgc gcttcgtcgg ccattgagat
     5581 caggacggac ggaaaaaccg tctcgtgccc gatgttgccg aggctcgtca cggcagccgc
     5641 acgcaccgcc gcgtctcgat catttgcggc cagagacgtg agagccgcca ccgaacgctg
     5701 cacctgaaat tgcgcgagtt gctgcgccgc agagacgcgc gcttccacgt ccgcgctgtc
     5761 gagcctttcg agcgcgtccg cgagcgtctc gtcttccatc cattcgggca gttgttcaaa
     5821 cgccgcgacg ggttcaaacc ctctgatctc gcccgtgaac ggatcaatcg caggttcggg
     5881 cagcgcaaac gaatcgccgt ctgcgctcgc ttcaaggctc aggcaatcca ccgagcacgc
     5941 gctgagcgca tgacctaaga gcgcgggcgg aacgttcgga tgacgccgcg ccatgtcgag
     6001 cagcgcgccg atggccgacg gcaaaccgag cgcgccgaga ctcttgaccg cttccgtgca
     6061 gacgatctgt tcgccgtcat agagcgcttc gagcagaaac ggcagagacg cacgcgaacg
     6121 catttcgccg agcgcgcgcg ccgccgacga acgctcgtaa gcgtcgggcg caagcagcac
     6181 cgacgcgctg tgccgcgcga cgaagccgta ttcttcgagc gcgaaacgcg cacgctcgcg
     6241 ttccgtctgg ccgagcgaat gtgaattgag caccttgagc agggaggctt cgatggcgcg
     6301 gcggtcgtcc gtcgcacgcg aggccagaac ctcgatgcgg tgcggctctc cggcgaccag
     6361 tttctccact tcctgatcga tctggtacgc cccgaagatc actgccggga cgttcatgtg
     6421 cgcgggcgtg gacgacttgc gctccgactt ctcaaggctc tgcgacgccg tgaccgccgc
     6481 ccctccgcct gtcggcacga gagcctgttt gtcttcggtg cgacgcgcgc tcgtgcgtcg
     6541 ttcgcccttg cgctgttcca gcagttgcgc ttcgccttcg aattcgacaa ccgtgctcgc
     6601 cgcttccgtc gtcttgcggc tctcgccttt cgccgggttt gaagtcgccg tcttgcgccg
     6661 cgagcgcagg aagatgaaca tgaaggcggc gaacccggtc gagaaacaga ccgacagcac
     6721 gaccagcaga cgcttcatcg aactcccgcc cgcaggctgc gcgacctgca cctgcatcgc
     6781 ggcgggcgaa gcgttttgtg tacttgtcgc gggcggctcg gctgaagccg gattcacaat
     6841 ttgcgtctca tgcgcagcct gcccgttggc gggtgcgttt tgcgcggcgg gagagactgc
     6901 gttgacgccg ttgttcgttc cattcgacgc tgccgttgcg ggttgcgtcg tgttgggtgc
     6961 cgtcgaaaat ttcagcgcgg cgggcggcgc gagcagcggc gtcaaattcg gcttcgccct
     7021 gtcgttcttc aacgcgacga cggactctct ggcctggacg cccgaagttg ccccgttgcc
     7081 gccgttcgcg cgcaacttcg aggcagcttg ttgcgcctcg cgcgccgtgg ctgtgcgcgt
     7141 gctctgcgcc ggagaatttt cgcgcgccgt ctccgctgcc gtgtgcaggc cgccgcccac
     7201 gacgagatcg agactgttga aacgtggctg gacatagacg ctgccgccgg gcttgaccgg
     7261 aacgacgaac tccaacgatt cgcccacgcg ccgcaccttc acaccgcgcg gcacgccgcc
     7321 cggatttccc cgccccttgt agacgaccac gtgaaagcct tcgctgtcct gccacgtctg
     7381 cgcgcggctg agcgggctgt cggcggcgat ggagacaacc gtgtccgagc cgttcgtgcg
     7441 cgtcgtaacg cccatcacgt tgacgccgga ctcttttccg gcgcgctgcg cctgcggcac
     7501 gacgagcggg aagatggtga cggcgaggcc gagcgaaacg caagacacgc cgatgcgtgc
     7561 gcggcgagaa attttatggc tgagatggcg catggctttt aaacctattt cggaaagtcg
     7621 gccacgctct ttcgagtttt ggcctgcatg agttctctgt gagagtcgag cgtgagacgg
     7681 acgctgtgag gcggaaagag gcggacggac gagatcaggc gcgtcggctc ggcaagcgtg
     7741 ctatgggatt ttgattggcg ttgcgctgat cggccttgct aaagtttggc ctcggcagcg
     7801 ctttgggaat gaacttcgga cgggtggcga cgcgcttcgg gagtctatcg ttgctcgtcg
     7861 ctgaatttgt aaccgatgcc ccagacggtg agaaccatct gcgggttttc gggattgtct
     7921 tcgatcttgg ctcgcaggcg attgatgtgc gagtcgatgg tgcggtcgta tccttcgtag
     7981 gagtagtccc agatcttctc cagcagatac tttcgcggaa agacgcggcc aggatttgag
     8041 gcaaagagcc gcagcagttc aaactctttc ggagtcagtt cgacggggcg gtcgccgacc
     8101 gtcacttcac gcttcgccgg atcgatacgc agtttgccac gcaggatcgg cgcttcgtct
     8161 cccccgctct ggctgcggga ggccaccttc acgcggcgga gcacgctctt gatgcgcgcg
     8221 tcgagttcgc gcagattaaa gggcttggtg atgtaatcgt ccgcgcccat ctccagaccg
     8281 agcaccttgt caaccacgtc gtccttggcc gtcaacatca ggatcgggat ggaaggagcc
     8341 ttctcccgga gttcacgaca gacggcgagg ccgtccattt tcgggagcat caaatcgagg
     8401 atgatcgcgt cagggtgatc ctgcaaggcc gattcgacgc ctgccacgcc gtccgccgcg
     8461 ctcctgacgt tgtacccggt cttcgagaaa tattcgctga tggccgtgga aatattctcg
     8521 tcgtcttcga tcaccagaag cgtcattttg cggtcaaccc attcaggctc ttcgccttct
     8581 gcgtctttgg cgcgtggatt gttggcagcg ggtttcgttg acattagggc tgtagtctta
     8641 acgaaaggac tgtgggaact ctttgtggta caaattaccg gagacgctgg tgcgccgccg
     8701 actgttgaga atataacagg acaagcccct taaactcaag caataagttg gccttcgacg
     8761 cggcgcgcgc cgcccgcgcc gtctccgccc cgcaggcgcg gcgggattcg cttgcaacag
     8821 ctttccccgg
//
LOCUS       DASWNS010000534         7378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_357494, whole genome shotgun sequence.
ACCESSION   DASWNS010000534 DASWNS010000000
VERSION     DASWNS010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357494"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1321
                     /locus_tag="VGB76_18350"
     CDS             89..1321
                     /locus_tag="VGB76_18350"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100915.1"
                     /translation="MSHQANEPAVDRPPVTADLTPAPAEAELTRQTRIGGFKIPTDAL
                     RAYTMLFALVAIWVYFNFATDGIFLDPRNFSNLMRQTAVTGVLAVGMLMVIVTGNIDL
                     SVGSLVGFIGGVAAFAQGTNSWGLVPTLAAAIIIGIAVAEAQGALVAYANIPAFIVTL
                     GGLLAWRGVVLGMTKGETIPITLPGFKAIGQQFVTRPVGLALALVAVAAIVWMNLRRH
                     RARERYALGKTSVLNLALGIVLPSLVVVLFIWRMNASEGIPIPVIILIVVALAGAFLT
                     QNTTFGRYLYAIGGNPDAARLSGINVRTHIWAVFCIMGALAGVASIIYTARVGSASPD
                     AGNLLELDAIAACVIGGTSLMGGRGTVFGACLGALFMASLDNGMSLLNVQPYSQFIIK
                     GAILVTAVGLDMLGRRQG"
     gene            1519..5058
                     /locus_tag="VGB76_18355"
     CDS             1519..5058
                     /locus_tag="VGB76_18355"
                     /EC_number="3.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004339 - glucan 1,4-alpha-glucosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009251 - glucan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan 1,4-alpha-glucosidase"
                     /protein_id="HEY0100916.1"
                     /translation="MKSKFVMNASSWCVLFVLAAGMFLSGARAQQQGKQGGNNLAPGA
                     PGMEAHWPSAGKNGVGTANTLESKVWFTLRAGVLTEIYYPTVDVANSQELQFIVVTAA
                     GGRVETESEDSTHRIEIIDPRALSFRQINTAKSGVWTITKTYMADPERHTVLIDVEFK
                     SRTPGAHALYVYYDPSLNNSGLHDSAWVQGDALVASDAGKTSALLSSTGFIETSNGYL
                     GTSDGLTQLRRTQGRLTNQYPRAADGNAVQVARVHGVRPFTLALGFGQGTEEALANAR
                     KSLQKGFARVRAEYERGWHEYLESLRRVEPKYQAQFDMAAMILRAHEDKTYRGANIAS
                     LSVPWGGGATANEPNVGGYHLVWSRDLYQVATALHALGDKAGADRALNYLFKVQQKAD
                     GSFPQNSWLDGRPFWGSLQMDEVAYPLILAYTLGRTDNETWTKHVKPAADFLVKHGPR
                     TPQERWEEKAGYSPSTIAAEIAGLVCAAEIARRNDAGASAGIYLAVADEWARNIERWT
                     ATSTGLHGEKNYYIRIAHVDDDPNDGDMMNAGNGGADYDEREYVDAGFLELVRLGIKR
                     ADDPLIAKSLAVVDRVLKVETPNGAAWYRYNHDGYGEMDDGRPWNFDGKYTGKGRLWA
                     LLSGERGQYELARGERAAARMRLDHMLGFANDGLMLPEQIWDRRESPRPEFVFGEGTG
                     SATPLAWTMAQFIRLAANLAEGRNLETPDIVAARYAAKPLPADAGGAASGDAEEEREP
                     VEAGASLNLGGSVRAEAGTRVYVWANGEAREVTGNGAAGTAEPDFRVPEGETTLVIGW
                     HKANGASALRRIRVRGMTKDELRKYETAQAAQQATPSFLERVQKAERSPLVEGERVTF
                     IYRGKARRVEVVGDFTSWSPRGMTLDDVAGTDLKYLTLKFAPDARVEYKFIADGEWTG
                     DPLSAQKLDNGVGGFNNFFTMPGYRPSAFAEAKWDLRGTIERLDAPTSLLEGGKRKLQ
                     VYLPPAYARAANERFPVLYLQDGSEYVTRARAAAIADQLIAGGRVAPFIIVFVDPVDR
                     FKEYWASDKFAAFMATELVPFIDARYRTRAVRDSRALMGASLGSVISVWTAVKYGDVF
                     ARVAGQSTAFQIDDERVVTTLAALDEMGRARPVKFYFDVGLLEPIWKVNRRVRVMLAG
                     KGYPVVYRETAAGHNWTNWRDRLADAFIGVWTD"
     gene            complement(5187..>7378)
                     /locus_tag="VGB76_18360"
     CDS             complement(5187..>7378)
                     /locus_tag="VGB76_18360"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0100917.1"
                     /translation="QLRLYQNGSDAAGATIEDKVRDVLNDDNIGPLDLLAGFTNASAI
                     TLVAAGLPAQPTAMQMLPIDINPNGVSNNNFVAAHEAAVVAAARAVPVRSDLTGANAP
                     VFVGAPVVAAAQPVANGAAQANVKRAQDAVQETSQAKTPNAQPMASAFPVNIPVLQPG
                     ESVTITFQVTVNLNIPNNVTSVSNRGRVTSSSFPNPAVDFVDTENKEGSTVTGGATVT
                     DILPRPTYTINNASVAEPASGSVNMPFTVALSHAYGSPVSVSFSTADGTATAAGGDYT
                     PTSGTLNFAAGQTVQTISVPVLANGDTSDENFTVTLSSPVNSVLGATVTATGTITEAN
                     PPGRVLISEVRTSGPGGAGDDFIELYNNQDVSQNISGWAIVKSGASCLSTPVIVAVIP
                     AATTIPARGHYLIVGTAYSLAAAAPGNITAAADIEADHNIGLFNTSNLSNLSTATRED
                     AVGFDINAGGGNNCDLLREGASLLSAGGSASEHSFARILTTGLPKETNDNATDFLLVT
                     TTPAVGVGNNLTPALGAPGPENLAAPIQRNGTIKSSLVDGTVAASAPPNRVRSGIIEP
                     GVPNAFGTLSIQRKFTNTLSVPVTRLRFRIVDLTTINNRPNGHADLRVLSSTGVVRNS
                     AGTIVRTVNGLTLEAPPQPNGGGLNSALTVVLSGGMLAPGNSIEVQFLLGVQEQGNFS
                     FYVNVEALPGPTGAAAPLGGANLKNGSTQKQGSVEETPAEPKQPQQQ"
BASE COUNT         1283 a         2133 c         2593 g         1369 t
ORIGIN      
        1 gcgacggggc atgcacaaca tgccgcgtaa gtttcgtggc cgtgtacccg cataagtttt
       61 aagtcgatac ttctcgacgg agcttcccat gagccatcaa gcaaacgaac ccgcagttga
      121 ccgtccgccc gtgacggctg atctgacgcc cgcgcccgcc gaagcggagt tgacgcggca
      181 gacgcgcatc ggcgggttca aaatcccgac cgacgcgctg cgcgcctaca cgatgctgtt
      241 cgcgctcgtc gccatctggg tctatttcaa cttcgccacc gacggaattt ttctcgaccc
      301 gcgcaacttc tccaacctga tgcgacagac ggcggtgacg ggcgtgctcg ccgtcggcat
      361 gttgatggtc atcgtgacgg gcaatataga cttgtcggtc ggctcgctcg tcgggttcat
      421 cggcggcgtg gccgccttcg cgcaaggcac gaacagttgg ggactcgtgc ccacgctcgc
      481 ggcggcgatc atcatcggca tcgccgtggc cgaagcgcag ggcgcgctcg tcgcctacgc
      541 gaacatcccc gccttcatcg tcacgcttgg cgggctgctc gcctggcgcg gcgtcgtgct
      601 cggcatgacg aagggcgaga cgatcccgat cactctgccg ggctttaaag ccatcggcca
      661 gcagtttgtg acgcgcccgg tggggcttgc gctcgcgctc gtcgcggtcg cggcgattgt
      721 gtggatgaac ctcaggcggc accgtgcgcg cgagcgttac gcgttgggga agacgagcgt
      781 gttgaatctc gcgctcggca tcgtgttgcc ttcgctcgtc gtcgtgctgt tcatctggcg
      841 catgaacgct tccgagggaa tcccgattcc ggtgatcatc ctgatcgtcg tcgcgctggc
      901 gggcgcgttc ctcacgcaga acactacgtt cggtcgttat ctctacgcca tcggcggcaa
      961 cccggacgcg gcgcggctct ccggcatcaa cgtgcggacg cacatctggg cggtcttctg
     1021 catcatgggc gcgctcgcgg gtgtggcctc gatcatctac acggcgcgcg tcgggagcgc
     1081 gtcgcccgac gcgggaaact tgctcgaact cgatgcgatt gcggcctgcg tcatcggcgg
     1141 gacgagtttg atgggcgggc gcgggacggt cttcggcgcg tgcctcggcg cgttgttcat
     1201 ggcgagtctg gacaacggca tgtcgctctt aaacgtgcag ccctattcgc agttcatcat
     1261 caagggcgcg atcctcgtca cggcggtcgg cctcgacatg ctcggacgca ggcaggggtg
     1321 aataaagcag tgacaagtga cgggtaacaa gtaaggggta acaagtgacg ggtgacaagt
     1381 gacaggtcga attcttcttt gcgccttcgc gcctccggcg tgagttggct tcttggcaat
     1441 cgtcagtctc acgcaggggc gcaggggcag gcgacgggat gtgcgttatt gacgaacaac
     1501 tgacgggaag agagaacaat gaaatctaaa tttgtgatga acgcatcttc gtggtgcgtt
     1561 ctttttgttt tggcggcggg catgtttttg agtggggcgc gagcgcagca gcaggggaag
     1621 cagggtggca acaatctcgc gccgggcgcg ccgggcatgg aagcgcactg gccgagcgcg
     1681 gggaagaacg gcgtcggcac ggcgaacacg ctcgaatcga aagtgtggtt caccttgcgc
     1741 gcgggcgtgt tgacggagat ttattacccg accgttgacg tggcgaactc gcaggagttg
     1801 cagttcattg tcgtgacggc agcgggcggg cgcgtcgaga cggagagcga agactcgacg
     1861 catcgcatcg agatcataga cccgcgcgcg ctctcctttc gccagatcaa cacggcgaag
     1921 agcggcgtgt ggacgatcac gaaaacttat atggccgacc ccgaacgcca cacggtcttg
     1981 attgacgtgg agttcaagag tcgcacgccc ggcgcgcacg cgctctacgt ctattacgat
     2041 ccttcgctga acaatagcgg cctccacgat tcggcgtggg tgcagggaga cgcgctcgtc
     2101 gcttcggatg ccggtaagac ttccgcgctt ttgtcgagca caggtttcat tgagacatcg
     2161 aacggctatc tcggcacgag cgacggcctg acgcagttgc ggcgtacaca gggacgcctc
     2221 acgaatcaat acccgcgcgc ggcggacggc aacgccgtgc aggtcgcgcg cgtacacggt
     2281 gttcggcctt tcaccctcgc gctcggtttc gggcagggca cggaagaggc tctggcgaac
     2341 gcgcgcaagt cgctgcaaaa aggtttcgcg cgcgtgcgtg cggagtatga gcgcgggtgg
     2401 cacgaatatc tggagtcgct gcggcgcgtc gagccgaagt atcaggcgca gttcgacatg
     2461 gccgcgatga tcctgcgcgc gcacgaggac aagacgtatc gcggggcgaa catcgcttcg
     2521 ctctccgtgc cgtggggcgg cggggcgacg gcgaacgagc cgaacgtcgg cggttatcat
     2581 ctggtgtggt cgcgcgatct ctaccaggtg gcgaccgcgc tgcacgcgct cggcgacaag
     2641 gccggggcag accgcgctct gaattacctg ttcaaagtgc agcagaaggc ggacggcagt
     2701 ttcccgcaga actcctggct cgatggtcgt ccgttctggg gttcgctgca aatggacgaa
     2761 gtggcctacc cgctcatcct cgcctacacg ctcggacgca ccgacaacga gacgtggacg
     2821 aagcatgtga agcccgccgc agactttctt gtgaagcatg gaccccgcac gccgcaggag
     2881 cgttgggaag agaaggcggg ctattcgcct tcgactatcg cggcggagat cgccggactc
     2941 gtctgcgcgg cagagatcgc gcggcgcaac gacgccgggg cgtcagccgg aatctatctc
     3001 gcggtggccg acgagtgggc gcgcaacatc gaacgctgga cggcgacttc caccggcctg
     3061 catggagaga agaattatta catacgcatc gcgcacgttg acgacgaccc gaacgacggc
     3121 gacatgatga acgcggggaa cggcggcgcg gactatgacg agcgcgaata cgtggacgca
     3181 ggctttctcg aactcgtgcg cctcggcatc aagcgcgcag acgatccttt gatcgcaaag
     3241 tcgctcgcgg tggtagacag agtgttgaag gtcgagacgc cgaacggcgc ggcgtggtat
     3301 cgctacaacc acgacggcta cggcgagatg gatgatgggc ggccttggaa tttcgacggc
     3361 aagtacacgg gcaagggtcg cctgtgggcg cttttgtccg gcgagcgcgg gcagtatgaa
     3421 ctggcgcggg gcgagcgtgc tgcggcgcgc atgcggctcg atcacatgct cggttttgcg
     3481 aacgacggat tgatgctgcc cgaacaaatt tgggacaggc gcgaaagccc gcgcccggag
     3541 ttcgtcttcg gtgagggcac ggggtcggcc acgccgctcg cgtggacgat ggcgcagttc
     3601 atacgtctcg ccgcgaatct cgcggaaggg cgcaaccttg agacgcccga cattgtggcg
     3661 gcgcgttatg ctgcgaagcc ccttccggca gacgcgggcg gcgcagcgag cggcgacgca
     3721 gaagaggagc gcgagcctgt ggaagcgggc gcgagcttga atttgggcgg cagcgttaga
     3781 gccgaggcgg gcacgcgcgt ctatgtgtgg gcgaacggcg aggcgcgcga agtgacaggg
     3841 aacggcgcag cgggcacggc ggagccggat tttcgcgtgc ccgaaggaga aacgacgttg
     3901 gttatcggct ggcacaaagc gaacggcgcg agcgcgcttc gacgcatcag ggtgcgtgga
     3961 atgacgaaag atgaactgcg taaatacgag acggcgcagg cggctcaaca ggcgacgccc
     4021 tcattcttgg agcgcgtgca gaaggccgaa cgctcgccgc tggtggaagg cgaaagggtg
     4081 acgttcatct atcgcggcaa ggccaggcgc gtcgaggtgg tgggcgattt cacgagttgg
     4141 agtccgcgcg ggatgacgct cgatgatgtg gcgggcacgg acttgaagta cctgacgttg
     4201 aagttcgcgc cggacgcgcg cgtcgaatac aagttcatcg ccgacggtga atggacgggt
     4261 gatcctctga gcgcgcagaa actcgataac ggcgtcggcg gcttcaataa tttcttcacg
     4321 atgcccggct atcgcccttc cgcgttcgcc gaggcgaagt gggacttgcg cggcacgatt
     4381 gagagactcg acgcgcccac ctccctgctc gaaggaggga agcgcaagct gcaagtttac
     4441 ctgccgcccg cttacgcgcg cgccgcgaat gaacgcttcc ccgtcctgta cctgcaagac
     4501 ggctcggagt acgtgacgcg cgcacgcgcc gctgctatcg cagaccaatt gatcgccggg
     4561 ggacgcgtcg cgccgttcat catcgtcttc gtcgatccgg ttgatcgctt caaagaatac
     4621 tgggcgagcg acaagttcgc ggcgttcatg gcgaccgaac tcgtgccctt tatagatgcg
     4681 cgctaccgca cgcgcgccgt gcgcgactcg cgcgcactga tgggcgcgag cctcggcagc
     4741 gtgatctctg tttggacggc tgtgaagtac ggggatgttt ttgcgcgcgt ggcgggtcag
     4801 tccacggcct tccagattga cgacgagcgc gtggtgacga cgcttgccgc gttagacgag
     4861 atggggcggg cgcggcctgt gaagttctac ttcgacgtgg ggctgctgga accgatctgg
     4921 aaagtgaatc gccgcgtgcg cgtgatgctg gcgggcaaag gctacccggt cgtctatcga
     4981 gaaaccgccg ccgggcacaa ctggacgaac tggcgcgacc ggctggcgga cgccttcatc
     5041 ggcgtctgga ctgattgagc gcgaaggtcg aaagcgggaa gtaaaagaag aagaaaaagg
     5101 gaaggccgga agcgtagatg cttccggcct tccctctgtt tgggcggcga gactcgggct
     5161 tgcgcgagag cctcgccggt tagtgtctat tgttgttgcg gctgcttcgg ttcggcgggc
     5221 gtctcttcga cgctgccctg tttctgcgtc gaaccgttct tcaggttcgc gcctccgagc
     5281 ggggctgctg ctccagtagg accgggcaag gcttccacgt tgacgtagaa gctgaagttg
     5341 ccctgttcct gaactccgag caggaactgc acttcgatgg agttgcccgg tgcgagcatg
     5401 ccacccgaca gcaccacggt cagcgcggag tttaagccgc caccgttcgg ttgcggcgga
     5461 gcttcgagcg tcagcccgtt caccgtgcga acgattgtgc ccgcagagtt tctgaccacg
     5521 cccgtcgaac tcaacacgcg caggtcggca tggccgttcg ggcggttgtt gatggtcgtt
     5581 aagtcaacaa tacggaagcg caaccgtgtg acgggtacgg agagcgtatt ggtgaacttg
     5641 cgctggatag agagcgtgcc gaaagcgttg ggcacgcccg gttcgatgat accgctgcgg
     5701 acgcggttgg gcggcgcgct ggcggccacc gtgccgtcaa cgagcgacga cttgatggtg
     5761 ccgttgcgtt gaatcggggc ggccagattc tccggccccg gtgcgcccag agcgggcgtc
     5821 aggttgttgc cgacgccgac cgccggtgtc gtcgtgacga gcaggaagtc ggtcgcgttg
     5881 tcgttcgtct ccttcggcag acccgtcgtg agtatgcgtg cgaagctgtg ctccgatgcg
     5941 ctgccgcccg cagacaacag gctcgcgccc tcgcgcagga ggtcgcagtt gttgccgcct
     6001 cccgcgttga tgtcgaagcc gacagcatcc tcgcgcgtcg ccgtcgagag attcgacagg
     6061 ttcgacgtgt tgaagaggcc gatgttgtgg tcggcttcaa tatcggcggc ggcggtgatg
     6121 ttgccgggcg cggcggcggc gaggctgtaa gccgtgccga cgatgagata gtggccgcgc
     6181 gccggaatgg tcgtggccgc cggaatgaca gcgacgatca caggcgtcga gaggcagctt
     6241 gcgccgctct tgacgattgc ccagccgctg atgttctgcg agacatcctg gttgttatac
     6301 aactcgatga agtcgtcgcc tgcgccgccg ggtccggagg tgcgaacctc agagatgagc
     6361 acgcgtccgg gcgggttagc ctccgtgatc gtgccggtcg ccgtgacggt tgcaccgagc
     6421 acgctgttga ccgggctgga gagcgtcacg gtgaagttct catccgaggt gtcgccgttg
     6481 gcgaggacgg gcacggagat ggtctgcacc gtctgtccgg cggcgaagtt gagcgtgccg
     6541 ctggtcggcg tgtagtcgcc gcccgctgcg gtcgcggttc cgtcggcggt cgagaagctg
     6601 acggagacag ggctgccgta agcgtggctg agggcgacgg tgaacggcat gttcacggaa
     6661 cccgaagccg gttcggcaac cgaagcgttg ttgatggtgt aggtcggacg cggaaggatg
     6721 tccgtgaccg tcgcgccgcc cgtgacggtg ctgccttcct tgttctccgt gtccacgaag
     6781 tccactgcgg ggttcgggaa actgctgctc gtgacccgtc cgcgattgga cacgctggtc
     6841 acgttgttcg ggatgttcaa gttgaccgtc acctggaacg tgatggtgac ggattcaccc
     6901 ggttgcagca cggggatgtt gacggggaag gcgcttgcca taggctgagc gttgggcgtc
     6961 ttcgcttgcg atgtttcttg gacggcgtcc tgagcgcgtt tcacgttggc ctgcgctgcg
     7021 ccgttggcga cgggctgcgc ggcggcaacg accggcgcac cgacgaagac gggcgcgttc
     7081 gcgccggtca ggtctgaccg gacggggacg gcgcgcgccg cggcgacaac ggctgcctcg
     7141 tgggcggcga cgaagttgtt gttgctaact ccgttggggt tgatgtcgat gggaagcatc
     7201 tgcatggccg tcggctgcgc cgggagtccg gcggcgacga gcgtgatggc cgaggcgttg
     7261 gtgaatccgg cgagcaggtc gagcggtccg atgttgtcgt cgttcagcac gtcgcgcacc
     7321 ttgtcttcga tggtcgcgcc cgccgcgtcg gagccgttct gatagagacg gagttggc
//
LOCUS       DASWNS010000535         4054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_460860, whole genome shotgun sequence.
ACCESSION   DASWNS010000535 DASWNS010000000
VERSION     DASWNS010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460860"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1218..1493
                     /locus_tag="VGB76_18365"
     CDS             1218..1493
                     /locus_tag="VGB76_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100918.1"
                     /translation="MSATKKKKKKKRRTALVVCRDRKEFWTTQAQFWQWMREGVIRKT
                     ADQPLTGAFHREHEELMCIMSNTVLNLAHPNHVREALSSRRLGLAKK"
     gene            1538..2686
                     /locus_tag="VGB76_18370"
     CDS             1538..2686
                     /locus_tag="VGB76_18370"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0100919.1"
                     /translation="MKAIKSNFTARVDRPKAVLLVSAKLVLLLCLFAPGASAQQQRQS
                     NPAPAAVTPTPASATASNVPVKETATAPAASSTTPTPSPASDGRYRIGAGDVLDIRFV
                     KNPDLSRDNVRVDNRGMIRMPMLSEDIPAACLTEGELAQSIAKLYLKYKRNPHIDVFV
                     KEYNSKPVAVMGAVVKPGRFQLQREIRLLELLTQVEGASERAGGRVQIVHTETVNACE
                     AGGAASAASRVGESEGADFSRNLAIFKLEDTLKGDPKANPIIRPGDIISISDAEQAYV
                     VGNVLRPGTITLKEPVTISRAIAMSGGLMPDTKSEQVKIIRQVPGSLEKIEVVVNLKA
                     ITKHQAPDVPLLAGDVVDVPTSGGKRLLRSLIGTIAPTVGQLPVQVIR"
     gene            2782..>4054
                     /locus_tag="VGB76_18375"
     CDS             2782..>4054
                     /locus_tag="VGB76_18375"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY0100920.1"
                     /translation="MKEDLTVISSPPESVTPGALAHHSPPSNGHYVAPHSVRGYALED
                     DGDEVQLRDYWRAVQKRLWMVLGIATLVTAFAAIYVAKKPDIYQAQARVQVDTENNQQ
                     YSASKTGAVVVNNYDPVYFSTQLQNLTGQGLLRRVVKTLDLEHNQAFLRPQTEQKRST
                     WQNVLRIVGLGRKDDGKGKVVQDNTLRTESVAPAIARDDLVEAARAAPYVGMIRADLK
                     VEPVRDTRVSSRETRLIDIRFTHPDPQVAAKIVNAIADTFVLSNLERKTETNNTTGDF
                     LQKRIAELQSNIRSSEERLINYAKNNQILSLDASQNTVVERLSGLNRQLLEAENERKS
                     AEAAYRAGLAPGAAAALASEGAKQAQESEGTLAKLKQRRAELLVENTEEWPEVKEINQ
                     QIAVLEGQVREASGKATSVVKTNLETRYRQAL"
BASE COUNT          940 a         1129 c         1175 g          810 t
ORIGIN      
        1 gcctcctgaa tcattatata gataaggggt gagcgaaaaa cgaaaacggg cgcattcatg
       61 atctcaaaga gagcgtattc cggtaaggaa tggtgcagac tctctttcgc acgcgaggga
      121 gcggaggctt ccggcgggca agagatgaaa actcgctgcc cgttttgggc gcaactcgtg
      181 tcgcccggat tattgaggct tcgatagggc gggggagaag ttatctggca ccgtagttgc
      241 tgatattttg aactcaccgc taaatcccgc tcgaacggga gctcggctgc taaaaatgtc
      301 tcggatagta gacggtttct gaagagggat gtccacaaaa gtagacgttt cttccccgcc
      361 tgaggtgttc aagtttggag tttcaccggg agccgatttt atttagccgg agtcggcagt
      421 ttcatatctg aaacgcggtg agtactatcg aattgtgagt tgacaagggt agcctgacag
      481 gttaaaaacg gcaccttttg cgagagggga agcgactcat caggccgctc ccatttatca
      541 ggggaggcgg cagtaaaatt cctctcttac tcacaacgca agcgttgttg ccgctgcctc
      601 tagccgcaaa ccacgcaaag ctcaattcct gaggccggtt ttgagcgctc ccgtggattc
      661 ttctaactat tatagtttaa ggaatccgtt aagcccggat gtgattttcc cgcccgatgc
      721 cttagagtat taccaggcca tttatatttt tggctttcat aaagggaagg ttttctgggt
      781 actatctact gattttctcc tctttcgggt taataaatta ctaaattccg tagagctata
      841 tcctttcccg aaggcgttca tgtgagctta aaagttaagc cacaaggggc tttcgttggc
      901 tcactaattg tagttgtcga tctttggcaa gacgtctacc gcagagagtc tctgggaagc
      961 acccggttgg ttgattaaag agagtccggc agcatttcgg ttcggactcg tttgataatt
     1021 cggtggtcgc ttcgctttta ggcgaggctt ccctcgcaag aaggcttttc cagcttcgtg
     1081 agggcacggc gtgacgggca tctttttcgc tcaagttttt cgagggagcg gatcaagaga
     1141 acctaaggac atgcggcggg tgaagtgtgt tcgttaaagg gcgtgcggaa tgagtgggag
     1201 cgagggggat atagcttatg agcgcgacga agaagaaaaa gaagaaaaaa cggcgcacgg
     1261 cgctcgtcgt ctgccgcgac cgcaaggagt tctggacgac gcaggcgcag ttctggcagt
     1321 ggatgcgaga gggcgtcatc cggaagaccg cagatcagcc gctgacgggc gctttccacc
     1381 gcgagcacga agaattgatg tgcatcatga gcaacaccgt cttgaacctt gcacatccga
     1441 accacgtccg cgaggcactc tcctcacgcc gtttgggatt ggcgaagaaa tagattaagt
     1501 atatcgggcg atactttaaa acgaagagat caaggttatg aaagccatta aaagcaactt
     1561 taccgcgcgt gtggatagac cgaaggcggt gctgctcgtt agtgcaaagc tagtgctctt
     1621 gctatgtctc ttcgcgccgg gcgcgagcgc gcagcaacag cggcagtcga accccgcccc
     1681 tgccgcagtc actcccacgc ctgcatccgc cacggcctcg aacgtgccgg ttaaagaaac
     1741 tgccactgct ccggcggctt ccagcacgac ccctacgccg tcccccgcct ccgacggtcg
     1801 ctatcgcatc ggtgccggtg acgtgctgga catccgcttc gttaaaaatc ccgatctttc
     1861 gcgcgataac gtgcgggtcg ataaccgcgg catgatccgc atgccgatgc tcagcgagga
     1921 catcccggcg gcgtgcctca cagaaggcga actagcgcag tcaatcgcga aactctacct
     1981 gaagtacaag cgaaacccgc acatcgacgt tttcgtcaaa gagtacaact cgaaacccgt
     2041 ggccgtgatg ggcgcggtcg tgaaaccggg ccgcttccaa ctccagcgtg agatacgcct
     2101 cctcgaactc ttaactcagg tggagggcgc gagcgagcgc gccgggggtc gcgtgcaaat
     2161 agtgcacacc gagacggtca acgcctgcga ggcggggggc gcagcctcgg cggcctcgcg
     2221 cgtgggcgag agcgagggcg cagacttcag ccggaacctc gccatcttca aacttgagga
     2281 cactttgaag ggcgacccca aagcgaaccc catcatccgc cccggcgaca tcatcagcat
     2341 ctctgacgcc gagcaggcct acgtcgtcgg gaacgtgctc cgtcccggca cgatcacact
     2401 gaaagagccg gtcaccatct cgcgcgccat cgccatgtcg ggcggcctca tgccggacac
     2461 caagagcgag caggtgaaga tcatccggca ggtgcccggg agtctggaga agatcgaggt
     2521 ggtggtgaat ttgaaagcca tcacgaagca ccaggcgccc gatgtgccgc tcttagccgg
     2581 ggacgtggtc gacgttccga cttcgggcgg aaagagactg ctgcgaagcc tcataggtac
     2641 catcgcccca acggtcgggc aactgcctgt tcaagttatc aggtgacttt agcttgacgc
     2701 ctgtattgtt gattcggtca tcggtcaacc cagagccgtg tccgccctcg cagagcatta
     2761 ccccgacgct caggtaacat catgaaagaa gatttaacgg tcatttcctc cccgccagag
     2821 tcagtgacgc cgggcgcact cgcacatcat tctccgccct cgaacggcca ttatgtcgct
     2881 cctcattctg tgcgaggtta tgctctggaa gatgacggcg acgaggtgca actgcgcgac
     2941 tactggaggg cggtgcaaaa gcgtctgtgg atggtgcttg gaattgcgac gctcgtgacg
     3001 gccttcgccg ccatctacgt agccaagaag ccggatatct accaggcgca ggcgcgtgtc
     3061 caggtagata cggagaacaa tcaacaatat agcgcgtcga agaccggcgc ggtggtcgtc
     3121 aacaattatg acccggtgta tttcagcacc caattgcaaa atctcacggg gcaggggctc
     3181 ctccggcgtg tcgtcaaaac cctcgacctt gagcataatc aggctttcct gcgcccgcag
     3241 accgagcaga agcgttcgac ctggcagaac gtgcttcgga tagtcgggct aggccggaag
     3301 gacgacggca agggaaaagt tgttcaggat aatactctgc gcaccgaatc cgtcgcgccc
     3361 gccatagcgc gcgatgacct cgtcgaggcc gcgcgtgccg ccccctacgt gggaatgatt
     3421 cgagcagacc tcaaagtaga gccggttaga gacacgcgtg tttcttctcg cgagacacgg
     3481 ctgatcgaca ttcgcttcac ccaccctgac ccgcaagtcg ccgcgaaaat cgtcaacgcc
     3541 atcgccgaca cgttcgtgct ctcgaattta gagcgcaaga ccgaaaccaa taacaccacg
     3601 ggcgatttcc tgcaaaagcg catcgccgaa ctgcaatcca acatccgcag tagcgaagag
     3661 cggctcatta actacgcaaa gaataaccag atcctctctc tcgacgcgag ccagaacacc
     3721 gtggtcgaac ggctgagcgg gctgaaccgg cagttgctcg aagctgaaaa cgagcggaaa
     3781 tcggctgaag cggcataccg cgccggcctc gctccgggcg cagccgccgc cttggctagc
     3841 gagggcgcaa agcaggcgca ggaatccgaa gggacacttg ccaagctgaa acagcggcgt
     3901 gcggaactcc tcgtagagaa caccgaagag tggccggagg tcaaagagat taaccagcag
     3961 atcgccgtcc tcgaagggca agtcagggag gcgagcggta aagccacgtc cgtcgttaag
     4021 accaaccttg agactcgtta ccggcaggct cttg
//
LOCUS       DASWNS010000536         4471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_202532, whole genome shotgun sequence.
ACCESSION   DASWNS010000536 DASWNS010000000
VERSION     DASWNS010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202532"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="VGB76_18380"
     CDS             complement(<1..1381)
                     /locus_tag="VGB76_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY0100921.1"
                     /translation="MTEPTHTPHTALSATLPQVCLDALRWHNKPDALNEKRGGEWQRL
                     SGEEVATRVRAIASGLASLGIRAGDRVALLSENRPEWSIADLAILSLGAVNVPIYTTQ
                     AVEQVRYILSDSKARLLFVSGKKVYRHARAGIEGYGKIERLIFFDADDAAGERADALT
                     LEALERAGRERAAQDPTEFARLLGAIKADDLATIIYTSGTTGEPKGVMLSHAAFVSNV
                     LAITSALPIRPDDVSLSVLPLSHIFERTVFYVFCYNGVSVYYAPSVDQLAEYLREVRP
                     TIMTAVPRLFEKVYHRIVKKGMSAEGWRRKLFLWALETGKRYAEFVDKRERVPPLLEL
                     QQDIASLLVFSKWREGVGGRLRFFVSGGAPLSPALSYAFLAAGIPILQGYGMTETCVT
                     SANRPEDNKVGSIGLPFPGIEMRLDESGEILVRGPNVMRGYYEHPQETAEVLDAKGWF
                     RTGDVGYKDE"
     gene            complement(1543..3120)
                     /locus_tag="VGB76_18385"
     CDS             complement(1543..3120)
                     /locus_tag="VGB76_18385"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0100922.1"
                     /translation="MTTSTSNASAAAAESVRTDDAPQAFDPTGAPPPPGRPPRRSAAS
                     APEPRTLASRFIILVLCLAVILSTLAFGAVQAWALGFFQLGAGLVVILWTVDAWRTRR
                     LRLSRNLLQLPLIGLFVVGLVQLLPLGGAGEAIGGLTKPPVNTLTLDPYATRFVLLQL
                     GALIIYFCAALVFTDSPARLRLLVRVITIFGFLLALFGMFQYFTNPSQLYWFRQPKFA
                     IPFGPFINRHHFAAYMELTLALPLGLLASGAIAVERRLLYIFAVLIMSVALVLTGSRG
                     GILSLAAEVLFIVAIAGLLRHRDKSETDESEAAQSRVRAALVRVGIGFALVVALLASA
                     ATLGGEESLSRLFGTVSADDPTSGRVQFWQGTWEVIKHYPVLGAGLGAFSVVYTRHDV
                     LNGATRLEQAHNDYLQILADAGIVGGMLGLLFIFALFRYGFSRFNSKDKFRRGVAAGA
                     LAGCFAVLVHSFFEFSLHMTSNALLFLVLAALATLNGRVEKPDTDKGRRSRRRRRSSS
                     SSSASSAETGEREARTA"
     gene            complement(3065..4387)
                     /locus_tag="VGB76_18390"
     CDS             complement(3065..4387)
                     /locus_tag="VGB76_18390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100923.1"
                     /translation="MKDQTKSIELRTVWARVLLLAPLALVLLGVWYGMRWCIGNTMAE
                     WLPDIGAAQAAARLAPDDPQSHFTMARLRERSFLPEELPEAARQYEEAARLSPNDFRF
                     FMELGRVRGLLGDVRGGEEALRRAVELAPTYPEPRWYLGNSLLRQGRTEEAFAELRRA
                     GDASPEKFRPQVLELSWRFYNADIPSVLGAVGNSAETRGQLMDYLINRKQLDDARQLW
                     QGFNADERKALRATGEKLMLRLLEAKRFHDMQAMHTELSAIDGTPSDAARERVLNGGF
                     ESAVGAPGTHPFEWQVVPLAGVQMGLDERVRQEGARSLRLAFNAASTINFRNISQLVA
                     VEPQTRYRLEYYTRTEELKGAATLLVEIVDAAQPDRVIVASEPLRIGTADWQASALDF
                     TTGAQTQALTLRVVSAQCPAATCPIFGKVWYDNFNLQRLSGGGGVRKN"
BASE COUNT          854 a         1448 c         1417 g          752 t
ORIGIN      
        1 gctcgtcttt gtagccgaca tcgcccgtcc gaaaccagcc cttggcatcc agcacttccg
       61 ccgtctcctg cgggtgctcg taataaccgc gcatcacgtt tggaccccgc acgagaatct
      121 cgcccgactc gtccagacgc atctcgatcc ccggaaacgg cagaccgatt gacccgacct
      181 tgttgtcttc cggacgattg gcggaagtga cgcacgtttc cgtcatcccg tagccttgca
      241 ggatggggat gccagcggcg agaaacgcat aggagagcgc gggcgagagg ggcgcgccgc
      301 ccgacacgaa aaagcgtaag cgtccgccca cgccctcgcg ccacttcgag aagacgagaa
      361 gcgaagcgat gtcttgctgc aactcaagga gcggcgggac gcgctcgcgt ttgtccacaa
      421 actccgcgta acgcttcccc gtctccaaag cccagaggaa taattttcgc cgccaccctt
      481 ccgccgacat gcccttcttc acgatgcggt gatagacctt ctcgaagagc ctcggcacgg
      541 cggtcatgat cgtcgggcgc acctcgcgca ggtattcggc gagttgatcc acggagggcg
      601 cgtagtaaac cgagacgccg ttgtaacaga agacgtagaa gaccgtgcgc tcgaagatgt
      661 gcgaaagcgg gaggacggag agcgaaacgt cgtccggcct gatcggcagc gcgctcgtga
      721 tcgcgaggac gttcgagacg aaggccgcgt gcgagagcat gacgcctttc ggctcgcccg
      781 tcgtgccgga agtgtagatg atcgtggcga ggtcgtccgc tttgatcgcg ccgaggagtc
      841 gtgcaaactc cgtggggtct tgcgcggcgc gttctcttcc cgcgcgctcc aacgcttcga
      901 gcgtcaaagc atcggcgcgc tctccggcag catcatccgc gtcgaagaag atgagccgct
      961 cgatcttgcc gtagccttcg atccccgcgc gcgcgtgacg atagaccttc ttgccggaga
     1021 cgaacagcag gcgcgctttt gagtccgaca ggatgtagcg cacctgttcg acggcctgcg
     1081 tcgtgtagat gggcacgttc accgcgccga ggctcaggat ggcgaggtcg gcgattgacc
     1141 attcgggacg attctccgac agcagcgcca cgcggtcgcc cgcgcggatt ccgagcgacg
     1201 ccagccccga cgcaatcgca cgcacgcgtg tcgccacctc ttcgcccgac aggcgctgcc
     1261 attcgccccc gcgcttctcg ttcaacgcgt ccggcttgtt gtgccagcgc aaagcgtcga
     1321 gacaaacttg cggcagcgtc gcggagagcg cggtatgcgg cgtgtgcgtt ggttcggtca
     1381 tagattaact ctggttcaag tcgggcggaa caggtttcag cttcgccgcg agcgagacgt
     1441 ttatagctca cgcgtggcaa ctcacgccgg aaattaaaag tcgtgcgcga aactaatttc
     1501 ccgaaagatg ctaccggcgt ttcttacttc ggcgtctctt acttaagccg tgcgcgcctc
     1561 tcgctcgccc gtttccgcgc tcgacgcgct gctgctgcta ctgcttctgc gcctgcgccg
     1621 cgagcgacgc cctttgtctg tgtcgggttt ttcgacgcgc ccgttcagag tggcgagcgc
     1681 ggcgagcacg agaaacagga gcgcgtttga ggtcatgtgc agagagaact cgaagaagct
     1741 gtggacgagc acggcgaaac aacccgcgag cgcgcccgcc gccacgccgc gccggaactt
     1801 gtctttggag ttgaagcgcg aaaatccgta gcgaaagagc gcgaagatga acagcaggcc
     1861 gagcatgccg ccgacgatcc ctgcgtctgc gagaatttgt aaataatcgt tgtgcgcttg
     1921 ttcgagccgc gtcgcgccgt tcaggacatc gtggcgcgta tagacgacgc tgaacgcgcc
     1981 gagtcccgcg ccgagaaccg ggtaatgctt gatcacctcc cacgtgccct gccagaactg
     2041 cacgcgccct gaggtcgggt cgtccgcgct caccgtgccg aacaggcgcg agagcgactc
     2101 ttcgccgccg agcgtcgccg cgctcgccag caacgccacg acgagcgcga agccgatgcc
     2161 gacgcgcacc agagccgcgc gcacgcgcga ctgcgccgcc tcgctctcat ccgtctcgct
     2221 cttgtccctg tgcctgagca agcccgcgat ggcgacgatg aacaacacct ccgccgcgag
     2281 gctcaagatg ccgccgcgcg atcccgtgag gacgagcgcg acggacatga tgaggacggc
     2341 gaagatgtag agcagtcgtc gttcgaccgc tatcgcgccc gaagcgagca ggccgagcgg
     2401 gagcgcgagc gtcagttcca tgtaggctgc gaagtggtgc cggttgatga agggtccgaa
     2461 ggggatggcg aatttcggtt ggcgaaacca gtaaagttgc gaggggttgg tgaagtactg
     2521 aaacatgccg aagagcgcga gcaggaagcc aaagatcgtg atgacgcgca caaggagacg
     2581 cagccgcgcg gggctatccg tgaagacgag cgcggcgcag aaatagatga tgagcgcgcc
     2641 gagttgcaac aggacgaagc gcgtcgcgta ggggtcgagc gtcagggtgt tgacgggcgg
     2701 tttggtcaac ccgccgatag cttcccccgc gccgccgagc ggcaacaact gcaccagccc
     2761 gaccacaaaa agaccgatga gcggcaactg caatagattg cgactcaggc gcagacgccg
     2821 cgtgcgccac gcatcgacgg tccagaggat gacgacaaga cccgcgccga gttgaaagaa
     2881 gccgagcgcc cacgcctgca ccgccccgaa ggcgagcgtg gagagtatca cggcgagaca
     2941 gagcacgagg atgatgaagc ggctggcgag cgtgcgcggc tctggcgcgc tggcggcgct
     3001 acggcgcggc ggacgccccg gcggaggcgg cgcgcccgtc gggtcgaacg cttgcggagc
     3061 gtcgtcagtt cttacggact ccgccgccgc cgctgaggcg ttggaggttg aagttgtcat
     3121 accagacttt tccaaaaatg gggcaggtgg cggcgggaca ctgcgcgctc acgacgcgca
     3181 gcgtcaacgc ttgcgtctgt gcgcccgtcg tgaaatcgag cgcggacgct tgccagtcgg
     3241 cggtgccgat gcgcaaaggt tcggaggcga cgatcacgcg gtcgggctgc gccgcgtcca
     3301 cgatctctac caagagcgtc gccgcgccct tcaactcttc cgtgcgcgtg tagtattcga
     3361 ggcgatagcg cgtctgcggc tcgacggcca cgagttgcga gatgtttctg aaattgatcg
     3421 tgctcgccgc gttgaacgcg agacgcagac tgcgcgcgcc ttcctgtcgc acgcgttcgt
     3481 cgagtcccat ctgcacgccc gcgagcggca ccacctgcca ctcgaacgga tgcgttcccg
     3541 gcgcgcccac cgccgactcg aaaccgccgt tcaagacacg ctcgcgcgca gcatcggacg
     3601 gcgtgccgtc aatcgcggag agttccgtgt gcatcgcctg catgtcgtga aaacgtttgg
     3661 cttcgaggag acgcagcatc agcttttcgc ccgtcgcgcg cagagccttg cgctcgtccg
     3721 cgttgaagcc ctgccacaac tggcgcgcgt cgtcgagttg tttacggttg atcaagtagt
     3781 ccatcaactg gccgcgcgtc tccgcggagt ttcccaccgc gccgagcacc gacggaatat
     3841 ccgcgttata aaaacgccac gacaattcga gcacctgcgg gcgaaacttc tccgggctgg
     3901 catcgcccgc gcgcctgagt tcggcgaacg cctcttcggt gcgtccctgt cggagcagcg
     3961 aatttccgag ataccagcgc ggctccggat atgtcggcgc gagttcgacg gcgcgacgca
     4021 aagcctcttc gcccccgcgc acatcgccga gcagaccgcg cacgcgcccc aactccatga
     4081 agaagcgaaa gtcgttcggc gacagccgcg ccgcctcttc atactgccgc gccgcctccg
     4141 gcaactcttc cggcaaaaaa ctccgctcgc gcaggcgtgc catcgtgaaa tgcgactgcg
     4201 gatcatcggg cgcgagccgc gccgccgcct gcgccgcccc gatgtccggc aaccactccg
     4261 ccatcgtgtt gccgatgcac cagcgcatcc cgtaccacac gcccaacaac acgagcgcga
     4321 gcggcgcgag taacaacacg cgcgcccaca ccgtgcgaag ttcgatgctc ttagtctggt
     4381 ctttcataag ggagcgatga gttgtcagcc gttaaccaaa ccatcaaaga gccgttagcc
     4441 cgtagccgga agttttctgt ttcggctgac a
//
LOCUS       DASWNS010000537         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_229493, whole genome shotgun sequence.
ACCESSION   DASWNS010000537 DASWNS010000000
VERSION     DASWNS010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229493"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..1585)
                     /gene="tilS"
                     /locus_tag="VGB76_18395"
     CDS             complement(239..1585)
                     /gene="tilS"
                     /locus_tag="VGB76_18395"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY0100924.1"
                     /translation="MKKPEAKPTANADAGTKRPLSRFARRLLQEWRRLGLPVRQERVV
                     VAVSGGADSTALLLACDELLRAGRVAVKLKVAHLDHELRGAAGASDARRVAELARELG
                     YECVVGRAAVARRASESRDNLEQAARRERYEFLGRAARGWEACAVAVGHTLDDQAETM
                     LLALLRGSGARGLGGMRERRSFDEAGDGLLLVRPLLDWARRVETLEYCESRGVSVRAD
                     EMNEDEQFARVRVRRRLLPLLETFNPRAVETLARTAGLLRRDADALETLGMELLREAR
                     RDARTETGAKRRGGAEAGQRVDEKNAVRDKAVRDEADAIADEREAMVNEGGSIVNEGG
                     SVADERDAIQDSPPREDSLSQCQDSLSRNDDSSALSVEVLRGASEGVRSYALRVWIAA
                     RRGHLRRIESVHLEAVGKLLEGERGGRVAELPGGCRVERRRGLLCFHTGEKKACES"
     gene            complement(1687..2760)
                     /locus_tag="VGB76_18400"
     CDS             complement(1687..2760)
                     /locus_tag="VGB76_18400"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0100925.1"
                     /translation="MQPKKRSLQRILASCLLALVILPLSASADNPHKRPFGDVQTLAP
                     FPVPPGSPEGIAVKGNKAYVSGPARFGTAGQPPSIVVVYDTRTGEKLETIQIQGEDLS
                     QEHANSCVAFDGDGRLYVVNTQLGIVRIDLGSGVQTVYASPVPNLPPFELPPLPNDLA
                     FDPAGNLYLTDSLQATIWRIPAGGGAPQVWFQDAALATPFGPNGIRLNPDRTKIYFVV
                     TAEGANPDMSFTGGRIYTLPLVDAPTAADLQVFHQYNGEGPDGIAFGNSGKLYVMLAS
                     PGFSGVSVLSPTGAEVARIGNPPAPPPFGLITPFNSPANGAFDKHGSLLVTNHTFVFP
                     GNESVLSVYVDDKESPLVKPNVP"
     gene            2983..3192
                     /locus_tag="VGB76_18405"
     CDS             2983..3192
                     /locus_tag="VGB76_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100926.1"
                     /translation="MRKLRAHGVSTSFIKELKQHGYERVPVEQLIRLKDHGVSASYIK
                     KMKSSGYGALSLDQLIDLEDRGVSR"
BASE COUNT          634 a         1091 c          943 g          592 t
ORIGIN      
        1 ataaagccgt cttcgaccac gcccagcacg gtcaactcgc gcccttcgta agcctgcgag
       61 atctccttcg ccagcgcggc cacgcgctcc gcgatctgcg ccgcgctgtg aatctccacg
      121 ataccgtctg ccatgcgttg aacctcggat aaactgtccg ttaaaagcaa agaccgctcg
      181 cgaaaactga gcggccatgt gcgacacact cgattctaaa tccccatccg accttttatc
      241 aactctcaca cgccttcttc tcccccgtgt gaaaacacaa caacccgcgc cgccgctcca
      301 cacggcaccc gcccggcaac tccgccacgc gcccgcccct ttctccctcc aacaacttcc
      361 cgaccgcttc gagatgcacg gactcgattc gacgcaagtg cccgcgccgc gccgcgatcc
      421 aaacgcgcag ggcgtaactt cgcaccccct cgctcgcccc acgcaacact tcgacgctca
      481 acgccgacga atcatcgttc cgcgacagcg aatcctgaca ctgcgacagc gaatcttccc
      541 tcggcggcga atcttggata gcatctctct catccgcgac agagccgcct tcattcacga
      601 tagagccgcc ctcattcacc atagcctctc tctcatccgc gatagcgtct gcttcatccc
      661 tgacagcttt atccctgaca gcgtttttct catctacacg ctgccccgct tccgcgcctc
      721 cacggcgttt cgcgcccgtc tccgtgcgcg cgtcgcggcg ggcttcgcgc aacaactcca
      781 tgcccagcgt ttccaacgcg tccgcgtccc gtcgcaacag tcctgccgtg cgcgcgagcg
      841 tctcgaccgc acgtgggttg aacgtctcca acaacggcaa cagacgcctg cgcacacgca
      901 cacgcgcgaa ctgctcatcc tcgttcatct cgtcggcgcg cacgctcacg ccgcgcgact
      961 cgcaatactc cagagtctcc acgcgccgcg cccagtcgag cagaggtcgc acgagcagca
     1021 gtccgtcgcc cgcttcgtcg aagctgcgtc gctcgcgcat cccgccgagt ccacgcgcgc
     1081 cgcttccgcg cagcaaagcc agcagcatcg tctccgcctg atcgtccagc gtgtgcccga
     1141 ccgcgaccgc gcacgcctcc cacccgcgcg ccgcgcgtcc cagaaactcg taacgctcgc
     1201 gtcgcgccgc ctgctccaga ttatcccgcg actcacttgc gcgccgcgcc accgccgcac
     1261 gtccgacgac gcattcataa cccaactcgc gcgccaactc cgcgacccgc cgcgcgtccg
     1321 acgcgcccgc cgcgccgcgc aactcgtgat cgagatgcgc cacctttaac ttcacagcca
     1381 cgcgtcccgc gcgcaacagt tcgtcacatg ccagcaggag cgcggtcgaa tccgccccgc
     1441 ccgataccgc gacgacgacg cgctcctgcc tgacaggcag accgagccgt cgccactcct
     1501 gcaacaaacg ccgggcgaag cggcttaaag gtcgcttcgt cccggcgtct gcgttcgcag
     1561 ttggtttggc ttccggcttc ttcatcgagc aggcggcgcg cgcctgtcaa gtgcggcgcg
     1621 cgcctgctca cagcatagaa ctctcaagac gtttcaggga agcgtcatag aactctcttc
     1681 gacgttttag ggaacgttcg gcttcacgag cggcgattcc ttgtcgtcaa catagacgct
     1741 cagcacgctc tcgtttccgg ggaacacgaa ggtgtgattc gtcacgagca gcgagccgtg
     1801 cttgtcgaag gcgccgttgg ccggggagtt gaacggcgtg atcaggccga agggcggggg
     1861 ggcgggcggg tttccgatgc gcgccacttc tgcacccgtc gggctgagga cggagacgcc
     1921 ggagaaaccg ggcgacgcca gcatcacgta gagtttgccc gaattgccga aggcgatgcc
     1981 gtccggccct tcgccgttgt actggtggaa gacttggagg tcagccgcgg tgggcgcgtc
     2041 aacgaggggc agcgtgtaga ttctgccgcc ggtgaatgac atgtcgggat tcgcgccttc
     2101 ggcggtgacg acgaagtaga ttttcgtgcg gtcgggattg aggcgaatgc cgtttggtcc
     2161 aaagggcgtg gcgagcgcgg cgtcctgaaa ccagacttgc ggtgcgccgc cgccagccgg
     2221 gatgcgccag atggtcgctt gcagcgaatc ggtcaggtaa agattgcccg ccgggtcgaa
     2281 ggccagatcg ttcgggagcg gcggcaactc gaaggggggc aggttcggca cgggcgacgc
     2341 gtagaccgtc tgcacgccgc tgccgaggtc gatacggaca atgccaagtt gcgtgttgac
     2401 gacgtagagg cgaccgtctc cgtcaaaagc gacgcaggag ttggcgtgtt cctggctgag
     2461 gtcttcgccc tgaatctgga tcgtctcaag tttctcgccc gtgcgcgtgt cgtagacgac
     2521 gacgatggag ggcggctgtc cagccgtgcc gaagcgtgcc ggaccggaga cgtaagcctt
     2581 attgcctttc acggcgatgc cttcgggcga accgggcggg acggggaacg gggcgagtgt
     2641 ctgcacgtcg ccgaaggggc gtttgtgcgg gttatcggca gacgccgaca gcggcagaat
     2701 aacgagtgcg agtaggcagg acgcaagtat cctttgtaac gatctctttt tgggctgcat
     2761 tatcttctcc tctaagtgag tattttaagg aatttcggat gcgtgactcg cgccgggtag
     2821 gaactgacgg ggcaatttgc ggcaacgcat cgtagtcaaa taaaacttcg ctcgcgggac
     2881 tccgtttacc gtcccaacta gtaaagcggg atttagaacg aaaaggtatc accccgcgca
     2941 actttatttt cgtgcgcccg cgaaaatatt tgcgtcgagc aattgcgtaa actgagagct
     3001 cacggcgtca gcacgagttt catcaaggaa ctcaaacaac acggctacga acgtgtcccc
     3061 gtagaacaac tcattcgcct gaaagatcac ggcgtctcgg ccagctacat caaaaaaatg
     3121 aagagcagcg gctacggcgc gctctcgctc gatcaactca tcgacctgga ggatcgcggc
     3181 gtgagcaggt aaggtcgagt aaaaggtcga gtaaaagata gaaaggcgag gggcgaaagt
     3241 gcagacggaa tatccgtctg
//
LOCUS       DASWNS010000538         5175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_98209, whole genome shotgun sequence.
ACCESSION   DASWNS010000538 DASWNS010000000
VERSION     DASWNS010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98209"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VGB76_18410"
     CDS             <1..472
                     /locus_tag="VGB76_18410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0100927.1"
                     /translation="FSMSSHLTRFTPGYPGTATYNGTTYTNILYQGSQLHFTVDPVVI
                     PLDATGVFTLTSTFTFEGSLIGFQMPNQAFLFSMTLSGQGIAELRFAPSFFGGRLGYG
                     LDSVTYTFQPAAAVPEPATLILLGTGLAGACANVRRRRRRHKASTHSAASADAL"
     gene            complement(569..1945)
                     /locus_tag="VGB76_18415"
     CDS             complement(569..1945)
                     /locus_tag="VGB76_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100928.1"
                     /translation="MRTHKSKRQTATAADALLSQCVLFALCVLLVHALGGRAAAPPPQ
                     VRSTGVASIKTDRAPHLPREVPKLPAAGVKFRDPVFGTEIMRVTDEATAPKDGGTSYS
                     YWATFNADNTKLIVMDPSVQHGASIYNFDPVSFTLGTRLPPVPYLPPGNIPPRLDDAC
                     WSSADPDALYVHQDLGTKIYRYKVSKQTFTLVGDVARVLPRGQNIQQMGMAADNDTFS
                     FTKRGAGPDYKPLGYAVYRASTNTIRFQHSEAINEVRIDKSGRYLYVNTNNQGVGKIE
                     GRVVDTDTGIVTDLTDDAPDHAPSHYDVGTGAVVGNGNYLVGISGRKLSTPRTFKKIL
                     DLSSEKNYGGFHLSMLADNEEWVLVSFYTPHQNSVMQGEVVQVATDGSGKVRRLFHHQ
                     SILVGYYDSPRANISRDGRFIAFTSNWGVKGGRRDMFIARIEPAPASAARPPAPPAAS
                     RPRRATTP"
     gene            complement(1966..2658)
                     /locus_tag="VGB76_18420"
     CDS             complement(1966..2658)
                     /locus_tag="VGB76_18420"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY0100929.1"
                     /translation="MNQIAADTLRARPARKKSGARRFVRVALALVLVWPLVAWAAARA
                     LIVADELPQGAEAIVVLSGASEYTERTRHAAELFRRGVAPKIILTNDDLRGGWNSEQQ
                     RNPYFVERAADELRRAGVPSDKIEALPEPVASTYDEAVRVRAYASARGVKALVFVTSA
                     YHSRRARWTMRRVLGESGVRVALAPVMPGSSSGEDGVQTPPASSWWLSARGWRSVVPE
                     YPKLVYYWLRYN"
     gene            complement(2698..4011)
                     /locus_tag="VGB76_18425"
     CDS             complement(2698..4011)
                     /locus_tag="VGB76_18425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY0100930.1"
                     /translation="MRLSVFGLGYVGCVSAACFAREGHEVVGVDVNSTKVEIINGGRS
                     PIVEAGVGELIGEMVAAGRLRATTDTSDAIRNSEVSLVCVGTPSNANGSLDLKYVRRV
                     CEEIGQELKTKTARHTVVIRSTMLPGTIETVVVPTLEEFSGKKSGSDFGVCINPEFLR
                     EGTSLKDFYAPPFTLIGADEAEVAATVGQLYAKIDAPLYTIGVRAAEMVKYTCNCFHA
                     LKVSFANEVGNICKELNIDSHEVMNVFCQDTKLNLSPYYLKPGFAFGGSCLPKDLRAI
                     TYKAKELDVDVPVLSSVLASNRRQIEHALEMVLATGRKRIGVLGFSFKAGTDDLRESP
                     MVTLIESLIGKGMQLAIYDRDVSLARLFGANKEYIEREIPHISQLMRDSIGEVLAASE
                     VLIIGNKAAEFREIETSLSDGQTVIDLVRLFEGRTSDDSYQGICW"
     gene            complement(4117..>5175)
                     /locus_tag="VGB76_18430"
     CDS             complement(4117..>5175)
                     /locus_tag="VGB76_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100931.1"
                     /translation="SLAFGLFTAALFIGVALSIGIYTVGDFKGWIVSASHQIGGNRGI
                     PQVAFGFARSFINMGNDNVDFKRYLLADPYSPVSLTDLFRLSLWKIALFYLFIAAILV
                     NLLRTPFGRRLLAWLALSGVPVFAFAFIWQGTVMERYVPWFPFLFPALAYALAERRAF
                     RFAKALALVFIAVMVWTNFGAMRKGALARHESAITERVRELQPLVSPQGIVFVGKDEL
                     ENFVRDYPFNPVNHHNDLRVYSFLQLGMPQAAAWQADVARRTLDAWRAGGEVFVSRRV
                     WAERPRREWDWVEGADPRVRWADVYAFFTQLETGRAVGGEDGFVLVARTPKNEQLLSR
                     VAAPETAADDSAARERIR"
BASE COUNT          957 a         1676 c         1568 g          974 t
ORIGIN      
        1 atttagtatg agttctcacc tcacgcgctt cacgccgggc taccccggca ccgcgacgta
       61 caacggcacg acttacacaa acatcctgta tcaaggcagc cagcttcact ttaccgtcga
      121 tcccgtcgtc attcctctcg acgccaccgg cgtgttcacc ctgacctcca ccttcacctt
      181 cgaaggcagt ctcatcggct tccagatgcc gaatcaggcg tttctcttca gcatgactct
      241 gagcgggcaa gggatcgcgg aactcaggtt cgcccccagc tttttcggcg gcaggctcgg
      301 ctacggatta gatagtgtca cctacacttt ccaacccgcc gccgccgtgc ccgaaccggc
      361 gacgctcatc cttctcggca ccggcctcgc gggcgcgtgc gcaaacgtgc gccggagaag
      421 gcgacgacat aaagcctcca cgcacagcgc ggccagcgca gacgcgttat aacgcgtagg
      481 caacacgcca cagtcttcat tcctaaatta tcagatagct cgacgcccgc atggcacctc
      541 tttgcaagcg tctaagatgt gcttcgtctt aaggcgtcgt tgcgcgtctg ggtcttgatg
      601 cggcgggcgg tgcaggaggt ctggctgccg acgccggggc aggttcgatg cgcgcgatga
      661 acatatcgcg gcgtccgccc ttcacgcccc aatttgacgt gaaggcgatg aagcgaccat
      721 cgcgcgagat gttggcgcgc gggctgtcgt agtagccgac cagaatcgac tggtgatgaa
      781 agagccgccg caccttgccc gacccgtccg tcgctacctg caccacctcg ccctgcatca
      841 cgctgttttg atgcggcgtg tagaagctga ccaagaccca ctcctcgtta tccgccagca
      901 tcgagagatg aaaaccgccg tagttcttct ctgaggagag gtctaggatt tttttgaagg
      961 tgcggggcgt cgagagtttg cgcccggaga tgccgacgag atagttgccg ttgccgacga
     1021 cagcgcccgt gcccacgtcg taatgcgaag gcgcgtgatc cggcgcgtcg tccgtcaagt
     1081 ccgtcacgat tcccgtgtcc gtgtcaacga cgcgcccctc aatcttaccg accccctggt
     1141 tgttcgtgtt gacgtaaaga taacgcccgg acttgtcgat ccgcacctcg ttgatcgcct
     1201 cggagtgctg aaagcgaatc gtgtttgtcg aagcgcgata gaccgcgtag ccgagcggct
     1261 tgtagtcggg gcctgcgccg cgcttcgtga acgagaaggt gtcgttgtcg gcggccatgc
     1321 ccatttgctg aatgttctgc ccgcgcggca gcacgcgcgc gacatcgccg acgagcgtga
     1381 aagtctgctt tgagactttg tacctgtaaa tcttcgttcc gaggtcttga tggacgtaga
     1441 gcgcgtcggg gtctgcggaa gaccagcagg catcatcgag gcgggggggg atgttgccgg
     1501 gcggcaaata ggggacgggc gggaggcgcg tgccgagcgt gaagctcacg gggtcgaagt
     1561 tgtagatgct cgcgccgtgc tgcacggacg ggtccatgac gatcagcttc gtgttgtccg
     1621 cgttgaaagt cgcccagtag ctatagctcg tgccgccgtc cttcggcgcg gtcgcctcgt
     1681 cggtcacgcg catgatctcc gtgccgaaga ccggatcgcg aaatttgact ccggcggcgg
     1741 gaagtttcgg cacttcgcgc ggcaagtggg gcgcgcggtc ggtcttgatt gacgcgacgc
     1801 ccgtggagcg tacctgcggc ggcggtgcag ccgcgcgccc gccgagcgca tgcacgagca
     1861 gcacgcagag cgcgaacaga acgcactgcg acaacaaagc gtctgccgcc gtcgcagtct
     1921 gccgtttgct cttatgtgtt cgcatttgcc ttgctccgcc cgccgtcaat tgtagcgcag
     1981 ccagtaatag acgagcttgg gatactccgg cacgacgctt cgccacccgc gcgcggacag
     2041 ccaccaactt gaggccggag gcgtctgcac gccgtcttcg ccgctgctgc tgccgggcat
     2101 cacgggtgct aaggctacac gcacgccgct ctcgccgagc acacgccgca tcgtccaacg
     2161 cgcacgccgc gaatgatagg cggaagtgac gaagacgagt gctttgactc cacgcgccga
     2221 cgcataggca cgcacccgca cggcctcatc ataagtgctc gccacaggct ccggcaacgc
     2281 ttctatttta tcggagggaa cgccagcgcg ccggagttcg tcagccgcgc gttcgacgaa
     2341 atacgggttg cgctgctgct cgctgttcca accgccccgc aggtcgtcgt tggtcaagat
     2401 gatcttcggc gcgacgcccc tgcgaaaaag ctccgccgcg tgtcgcgtgc gttccgtata
     2461 ctcgctcgcg ccgctcagga cgacgatggc ctccgcgcct tgcggcaact cgtccgcgac
     2521 gatgagcgca cgcgccgcag cccacgcgac gagaggccac acaaggacga gcgcgagtgc
     2581 gacgcgcacg aaccggcgcg cgccactctt cttcctcgca gggcgcgcgc gcagagtgtc
     2641 agccgcgatc tggttcatgc tctttgggcg ggcggcgggc gttcgccgca cgcgctgcta
     2701 ccagcagatg ccctgatacg agtcgtcgct cgtgcggcct tcgaacaggc ggacgagatc
     2761 gatcacggtc tgcccgtcgc taaggctcgt ttcgatctca cgaaactccg ccgccttgtt
     2821 gccgataatc aacacttccg aggctgccag aacttccccg atgctatcgc gcatgagttg
     2881 cgaaatgtgc ggtatctcgc gctcgatgta ctccttgttt gcgccgaaca ggcgtgcgag
     2941 cgaaacgtcg cggtcgtaga tggcgagttg cattcccttc ccgatgagcg actcgatcaa
     3001 ggtgaccatc ggcgattcgc gcaggtcgtc cgtcccggct ttgaaggaga agccgagcac
     3061 gccgatgcgc ttgcggcctg tcgccagcac catctccagc gcgtgctcga tctgtcgccg
     3121 gttgctcgcc aggacggacg acaacacagg cacgtccacg tccagctctt tcgccttgta
     3181 agtgatcgcg cgcaaatctt tcggcaggca cgaaccgccg aaggcgaagc cgggtttcag
     3241 gtagtagggc gacaagttca acttcgtgtc ctggcagaac acgttcatca cttcgtggct
     3301 gtcgatgttc agttccttgc agatgttccc gacctcgttg gcgaagctga ccttgagcgc
     3361 gtgaaaacag ttgcaggtgt atttgaccat ctcggcggcg cggacgccga tggtatagag
     3421 cggcgcgtca atcttcgcgt agagttgacc gaccgtggcc gccacttccg cttcgtccgc
     3481 gccgatgagc gtgaagggcg gcgcgtaaaa atctttgagc gaagtgccct cgcgcaagaa
     3541 ttccgggttg atgcagacgc cgaaatcgct gcccgatttt ttacccgaaa actcttcgag
     3601 cgtcggcacg acgaccgttt caatcgtgcc cggcagcatc gtcgaacgga tgacgacggt
     3661 gtgccgcgcc gtcttggtct tcaactcctg cccgatctct tcgcacacgc ggcgcacgta
     3721 cttgagatcg aggctgccgt tcgcgttgct cggcgtgccg acgcagacga gcgacacttc
     3781 cgagttgcgt atcgcatcac tcgtatcggt cgtcgcgcgc agacgcccgg cggcgaccat
     3841 ctcgccgatc aattcgccga cgcccgcttc gacaatcggg ctgcgcccgc cgttgatgat
     3901 ctcgactttg gtggagttca cgtcaacgcc caccacttcg tgcccctcgc gcgcaaagca
     3961 cgccgccgac acgcaaccca catagcccag tccgaacacg ctcaatctca tatttattcc
     4021 cgccgccttc ttttcgtcgc tcacgaatat gacgcttgcg aatgttgatg cctgcaaatg
     4081 ttgacgcgcg caacagttct acggcgtcac gcttatctac cttatccgtt cccgcgccgc
     4141 gctgtcatct gccgccgtct ccggcgcggc gacgcggctc aaaagttgtt cgttcttcgg
     4201 cgtgcgcgcg acgagcacga agccgtcttc gccgcccacc gcccgccccg tttcgagttg
     4261 cgtgaagaac gcgtaaacgt cagcccagcg cacgcgcggg tctgcgcctt ccacccagtc
     4321 ccactcgcgt cgcggacgct ccgcccacac gcggcgcgag acgaacactt cgccgcccgc
     4381 gcgccacgca tcgagcgtgc gtcgcgccac gtccgcctgc cacgccgccg cctgcggcat
     4441 gccgagttgg aggaaggaat agacgcgcag gtcgttgtga tgattgaccg ggttgaaggg
     4501 ataatcgcgc acgaagtttt cgagttcatc cttcccgacg aagacgatac cctgcgggct
     4561 gacgagcggc tgcaactcgc gcacgcgctc cgtgatcgcg ctctcgtggc gtgcgagcgc
     4621 gccctttcgc atcgccccga agttcgtcca gaccatcacc gcgataaaga cgagcgccaa
     4681 ggctttggca aagcgaaacg cgcgccgttc ggcgagcgcg taagcgagcg cgggaaagag
     4741 gaacggaaac cagggcacat aacgctccat caccgtgccc tgccagatga acgcgaaggc
     4801 gaagaccgga accccgctca aggccagcca agcgagcaga cgccgcccga agggcgtgcg
     4861 caggaggtta acgagaatcg ccgcgatgaa gagatagaag agcgcgattt tccacagact
     4921 gaggcggaag aggtctgtga gcgagacggg gctgtaaggg tctgcgagca ggtagcgttt
     4981 gaagtcaacg ttatcgttgc ccatgttgat gaaggagcgc gcgaaaccga acgcgacctg
     5041 cggtattccc cggttgcccc cgatctggtg cgaggccgac acgatccagc ccttgaaatc
     5101 gcccaccgtg taaatgccga tgctcaaggc gaccccgatg aagagcgcgg cggtgaacag
     5161 cccgaacgcg agcga
//
LOCUS       DASWNS010000539         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_513446, whole genome shotgun sequence.
ACCESSION   DASWNS010000539 DASWNS010000000
VERSION     DASWNS010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513446"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..1328)
                     /locus_tag="VGB76_18435"
     CDS             complement(285..1328)
                     /locus_tag="VGB76_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100932.1"
                     /translation="MKKRIRSEFIWKCGMGARLAVALLLLQSSWFAAPVARAATQDTT
                     QNPTTDVQRKQDGNIKAKGAFTCDFALPGDFPFSQVAPVIERDRMYMAERPGMQRKQI
                     PMRIDFSNGNLLTGGRYLFDTEQDAAKYRSWVENDFVLDGTHFFARPYFLNPDCRSWG
                     VVGAHNIKDIQQQIIMRTERWSVPHESQMHLLKQSWPLILQEAEQRGLTGVWLLYNEQ
                     EQLAQLVYFADRVVPQNPSTFDFASLAALEFGTPLGTVFDTQPEWIRTFDRTQWVLTV
                     WFPFELGDRGQPSLWPNSPPLPEPYPGDGVCEVSRGENSVNAPGDCAPTAGNGVCDAG
                     ENTQNTPGDCRLP"
     gene            1535..>2857
                     /locus_tag="VGB76_18440"
     CDS             1535..>2857
                     /locus_tag="VGB76_18440"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0100933.1"
                     /translation="MDTELNSHFYEFGPFRLDTVERLLLRRNTPVTLTPKVFDILLML
                     VRNSGRALEKEVFMREIWPDSFVEEGNLNRNISTLRKALGDGQAGQRFIETIPKRGYR
                     FVASVRETGAPTTDARSIAVLPFKALLEQEGDERYLGLGMADALIIKLSNVGKINVRP
                     TSAVLKYTAAHQDIRVIGRELKVETLVDGRVQKIGNRVRVTVQVVNTVDGATIWAEKF
                     DEKFTDIFTVQDSISEQVASALTLKLSGDERKSLSKHYTESSDAYQSYLKGRYYWNKM
                     TPEGLRRSLDCFADAIEQDPHYALAYAGMAASYVHMEIYGFMLPREAMPKARSAALKA
                     IELDETVAEAHASLALVRMFYERDWPEAEREFEQALELNPSYAAAHDWFAIYLMATGR
                     TDEALTSIRKAQALDPLSLIINTDVACALYYTRQFDASIRQCRWVLETE"
BASE COUNT          622 a          782 c          827 g          626 t
ORIGIN      
        1 aaaaatattt tggcctcaat tagtagtgct taagagtcat ctgctgcttc aatatcttgt
       61 atagcctgaa agttttcgca acacaaccca gccgactaca tctccggcgg aggctatacc
      121 tcctggtgga tcaagcgaca atagaagtgt ttgaagtcat gattcggcgc ggccacgcac
      181 ggctcagtgc cgtttgataa cgtgaccgcg ccggtcatat tgttgctact tttcctgccc
      241 gcgttcgcag ccctctcgac agatcatcca acggctaaag aagattatgg aaggcggcaa
      301 tcgccgggag tgttctgcgt gttctcgccc gcgtcgcaga cgccgttacc cgctgtcggc
      361 gcgcaatcgc ccggcgcgtt gacactgttc tcgccacgac tcacttcgca cacgccgtcg
      421 ccggggtagg gttcgggcaa aggcggcgag ttcggccaga gcgagggttg ccctcggtcg
      481 ccgagttcga aggggaacca cacggttaag acccattgcg tgcggtcgaa cgtccttatc
      541 cattccggtt gcgtgtcaaa gacggtgccg agtggcgtgc cgaactctag cgctgcgagg
      601 cttgcaaaat cgaacgtgga gggattttgc gggacgaccc ggtcggcgaa gtagacgagt
      661 tgtgcgagtt gttcctgctc gttgtagagc agccagacgc cggttagccc gcgttgttcc
      721 gcctcctgca aaatcaaagg ccaactctgt ttcaacaagt gcatctgact ttcgtgcggc
      781 acgctccaac gttccgtccg catgatgatc tgctgttgga tatctttgat attgtgcgcg
      841 ccgacgacgc cccacgaacg acaatcgggg ttgagaaagt aggggcgtgc aaagaagtgc
      901 gtgccgtcga ggacgaaatc attttcaacc catgatctat atttcgccgc gtcctgctcg
      961 gtgtcgaaca gatagcgtcc gcccgtcaga agattgccgt tggaaaaatc gattcgcatc
     1021 ggaatctgct ttcgctgcat gccggggcgt tcggccatgt acatccggtc gcgctcgatc
     1081 accggagcca cctggctgaa aggaaagtcg ccgggcaggg cgaaatcgca ggtgaacgcg
     1141 cctttcgctt taatgttgcc atcctgtttt cgttgcacgt cggtcgtcgg gttctgcgtt
     1201 gtatcctgcg tcgcggcacg ggcgacgggc gcggcgaacc aactcgactg caacagcagc
     1261 agtgcgacgg caaggcgtgc gcccatgccg cacttccaaa tgaattcgga acggattctc
     1321 tttttcatat caactccttc tatggttgaa cgtaattcgg tttttaaatt tccgacctgt
     1381 tttgctcctg ccagcggttg ggcttacaat gtgcgggaac atcgaacacg ctctttgtgc
     1441 agcccctcgc ttcggggcgg acaataagct gtagcaacct cggaagtcct tacagcacgc
     1501 cgaaaaattc ccgaaaaagt tccgaaagaa gcgcatggac actgaactca acagccattt
     1561 ctacgaattc ggtcccttcc gtctcgacac cgtcgaacga ctgctgttac gccgtaacac
     1621 tccggtcacg ctgacgccga aagtcttcga tattcttctc atgctcgtgc gaaatagcgg
     1681 gcgggcgttg gagaaggagg ttttcatgcg ggagatttgg ccggacagct ttgtcgaaga
     1741 agggaatcta aatcgcaaca tctccacact caggaaggct ctcggcgacg gtcaggccgg
     1801 acagagattc atcgagacta ttccgaaacg cggctatcgt ttcgtcgcca gcgtgagaga
     1861 aaccggcgcg ccgacgacag acgcgcgctc gattgccgta ctgcctttca aggcgttgct
     1921 cgaacaggag ggcgacgagc gatacctcgg tttaggcatg gccgatgcgc tcattatcaa
     1981 actgagtaat gtcggcaaga ttaacgtccg cccgacgagc gcggtgctca agtatacggc
     2041 ggcacatcag gacatccgtg tgattggtcg ggaattgaaa gtggagacgc tggttgatgg
     2101 tcgtgtgcag aagatcggta atcgtgtacg tgtgacggtg caggtcgtca acaccgtcga
     2161 tggcgcgacg atctgggcgg aaaagttcga cgaaaagttt acagacattt tcaccgtgca
     2221 ggactccatc tccgagcagg tggcgagcgc cttgacgctg aaattaagcg gcgacgagcg
     2281 aaagtctctc agtaagcatt acacggagag cagcgacgct taccagtcat atctcaaggg
     2341 gcgttattac tggaacaaga tgacccctga gggcttgcgt cgaagcctcg actgttttgc
     2401 cgatgctatc gagcaagacc cgcactacgc gctggcttac gcgggcatgg ccgcctcata
     2461 tgtgcacatg gaaatctatg gcttcatgct tccgcgcgag gccatgccga aggccaggtc
     2521 ggcagcgttg aaggctatcg agttggacga gacggtggcc gaagcgcatg cttcgctggc
     2581 gctcgtgaga atgttttatg aacgcgattg gccggaagct gaaagggagt tcgagcaggc
     2641 tctcgaactc aatccgagct acgccgcggc gcatgattgg ttcgccatct acctgatggc
     2701 gacggggcgg actgacgagg cgttgacctc gattagaaaa gctcaagcac tcgacccgct
     2761 ctcgctaatc atcaacacgg atgtcgcctg cgcgctctac tacacccgcc agttcgatgc
     2821 ttcgatcaga caatgccgat gggtgctcga aaccgaa
//
LOCUS       DASWNS010000540        22540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_461013, whole genome shotgun sequence.
ACCESSION   DASWNS010000540 DASWNS010000000
VERSION     DASWNS010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461013"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VGB76_18445"
     CDS             complement(<1..989)
                     /locus_tag="VGB76_18445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0100934.1"
                     /translation="MKKWLQENLPFPLKLASKQLLVATVGAFVRLAAFGYNSGRRILR
                     LAAKHGLDVDKVWNVTYEPEFAPAQLPVWSAQDFLFLTETLSAAEAHNAPRASDRQPR
                     TSIVIPVYNKAGFTFQCLRSLLREVDFAETEVIVVNNASTDETAKLLSHFQSLVTVVN
                     NEENRGFVDACNQGAAVARGRYLVFLNNDTVVLPGWLKHLLETVEGDASTGAVGSMFL
                     YEDGRVQEAGAGVWQNGAAFHYGWGGSPDDRRYNFAREVDYCSGASLLVRRDLFARLG
                     GFDTRYAPAYYEDVDICFGVRSLGYKVVYQPMSRVIHHEGVTAGRDTGVGFKR"
     gene            complement(1002..2342)
                     /locus_tag="VGB76_18450"
     CDS             complement(1002..2342)
                     /locus_tag="VGB76_18450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HEY0100935.1"
                     /translation="MKPIVKIENVSKRYRLGGLHPGYLTFRDMVGGALLTPFRRNGGG
                     DAGQVNERRRDFWALRDINLSVQPGELIGVIGRNGAGKSTLLKILSRVTKPTTGRVEM
                     YGRVGSLLEVGTGFHAELSGRENVFLSGAILGMRRAEIERKFEEIVAFSELEKFIDTP
                     VKRYSSGMYVRLAFSVAAHLEPEILIMDEVLAVGDAAFQQKCLDKMHAIRQHGRTIFF
                     VSHSMPAVTRLCRRVILLHAGQIVSEGEPQAVVNDYLSASWNITAECEWKTPADAPGN
                     DLVRLRRVRVLDEAGALATAFDIRRPVHIEITYDVLAAGHILTPHVGLVNEEGVRAFS
                     SHDTSDEWRHTPREVGRYASVIALPGNFLSEGTLRAHVSIMSHTPTTTLHAQARNIVA
                     FQMIESSQPGESARGDYIGPIPGVVRPLLDWTTTRFKPTPPGEHHATTGTLERS"
     gene            complement(2369..3316)
                     /locus_tag="VGB76_18455"
     CDS             complement(2369..3316)
                     /locus_tag="VGB76_18455"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0100936.1"
                     /translation="MDSHTSEPIASTPQPAATAPLTHASSPAVTTANAASDAVITLPD
                     RPLILIESEDAGVQFAFNDLWAYRELLYFLTWRDIKVRYKQTALGAAWAIIQPLATML
                     LFTLVFNRLAGLDTGRVPYPLFAYTGLLIWTFFSNAVTNSTNSLLNNTNLITKVYFPR
                     AFIPAAAVGAGLVDFAIAGLILVGLILYYGLAVNSGLALLPLFLLLAVMLALAVGMLI
                     SALTVKYRDLRHAVPFLIQFWMFASPVIYPSHRVPGAWRHLIALNPLTGIIEGFRAAL
                     TGGTFDRTATLLSIIVTLVALAFSFYLFRRIEETFADLI"
     gene            complement(3526..4521)
                     /locus_tag="VGB76_18460"
     CDS             complement(3526..4521)
                     /locus_tag="VGB76_18460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0100937.1"
                     /translation="MLNAPANTPGATETTGEPTVSVVVPSYNHEAFIEKTLRSIFRQT
                     LLPRELVVIDDGSTDGSVRIIERVLRDSPVAASAFVARENRGLCATLNEGFALAARGR
                     YFAYLGSDDLWFPQFLQSRVEILEARERAVLAYGNAYSIDAGDRIIDNTADWARYRDG
                     SVRLMLLETLAPLSPTVVYRRDALQAHRWNEAAGLEDYELYLRLSAEGEFAFDGRVLS
                     AWREHGSNASRNLRFMLREKLAAQQRVAARLGLSAEELAEFQTLARFRSAQEFMRRGE
                     KLEALKLASRNLRGARTRGEAFKFVAGLLTPHRLLNWRRGRARARAGARYGSLQI"
     gene            4555..5688
                     /locus_tag="VGB76_18465"
     CDS             4555..5688
                     /locus_tag="VGB76_18465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0100938.1"
                     /translation="MRVLHLLDSLNRGGAETLCLDVCRNARRNSLDLTFAATGGGELE
                     DDFRQSGADFVRLARTLPLDPKLIKQLRGLIDEREIEVVHCHQAVEALHAYLATRGGQ
                     RKGRRVKRVLSFHLCTADAKNRLALKFLAPRMDANVAVSRELLSCLGAEGGFQIGRNF
                     HVVYNGVDASRLDATGGGGDLRAELRLTKREVLFGMVGNFYADRRKDQLTVCRALPEL
                     FRQVPEAHFVFAGGYEENASGIYQECVNYCREQGIAGRVHFAGKRADVACVLDALDIF
                     VFSSRKDSFGVAVVEAMLAGVPAIVSDIGALLEVTRGGEHASIFRTGDADDLARKMVA
                     VACDPVRQRELGEQARRWARREFGIETHIGNLIKLYESLAGAE"
     gene            5728..6909
                     /locus_tag="VGB76_18470"
     CDS             5728..6909
                     /locus_tag="VGB76_18470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0100939.1"
                     /translation="MNGKRPRVRVLMGMPDRESLGGPAACEPPFVAGLRELGAEVVEE
                     TYVYGERPGGTTFRQRVLRVLATGRRLRRTLRAGEFDIVHLNSSFDTMALMRDAATLR
                     LLLPARAKIFIKFHGSDAALFETKNSALRALVRFVVAHADGIGLLSSEERGNFIRAGV
                     DGRKLFVVKNVVTGAEDAGHARTEHGAGSTPHASAAQAQSPNLRAKLGVDEETPLLLF
                     IARFIPAKGLTDVINACAILRDAGRAFELLCLGDGEARGAAEAETARLKLNERVRFFG
                     YVPETETAEFYAGSTMLVFPTYHYEGFPMVIFKSVAAGLPIITTRIRAAADYLREPDN
                     CLWVEPQNPRLLAEKITQLLERAETRALMRRHNLELASRFTARVVAPEYLEIYNQLTN
                     A"
     gene            6935..9124
                     /locus_tag="VGB76_18475"
     CDS             6935..9124
                     /locus_tag="VGB76_18475"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HEY0100940.1"
                     /translation="MKQILQNYKTGEVSVADVPPPIAQRGRVLVRTAASLISAGTERM
                     AVELGRKSLLGKARERPDLVRQVIKKAQREGLLNTFNAVQTKLNAANALGYSAAGIVT
                     DVGEDVPGFSAGDRVACAGAGFASHAEVLSVPKNLCVRLPDAVDFEAASFGTLGAIAL
                     QGVRLAELTLGESVVVIGLGLLGQLTVQLLKANGCRVFGIDLDPSKVELARTLGADDG
                     CVSGGETLQAVLDWSRGRGADAVLITAATPSDQPVELAGEISRLKGRVVAVGLVGMNV
                     PRKIYYERELTLKVSMSYGPGRYDPEYEERGHDYPFAYVRWTEGRNLEAFLDLVAAGS
                     VRIAPLITHRFPIEDGERAYNLIAGETKEPYLGIVLSYDTGRELVRSIELRGRDGKTN
                     ASSARAPGAASVHVGMIGAGSYAQKFLLPNFKANGAAFQSIATASGINARGIGEKYDF
                     ARVAASAEEVIADPDANLIVIATRHDSHAELARRALAAGRHVFVEKPLALNDEELDGV
                     LEAASASNGGRLLVGFNRRFSPHAREAAHAFAARREPLSILYRVNAGRIARTSWIQDE
                     REGGGRIIGEVCHFIDLMHFLTGAVTTRVYAEAIASRNEEIVDEDSVLITLRFSDGSN
                     GQIAYLAEGDRALAKERIEIFGGGKTFVIADFRSAVLYEGGRERRMKLRAQDKGQAEE
                     VRALCAMVREGTPAPIALQDLAATTRATFRIRESLRAGEPKTVTSDR"
     gene            9220..11196
                     /gene="asnB"
                     /locus_tag="VGB76_18480"
     CDS             9220..11196
                     /gene="asnB"
                     /locus_tag="VGB76_18480"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY0100941.1"
                     /translation="MCGIAGLVSQQPERRIGAMLKSIEHRGRDDEGVWTSASVDGEGR
                     RACLGHRRLSIIDPSEAGHQPMQYADGRYSVTFNGEIYNYRELRRELEGRNHRFRTDC
                     DTEVLLAAYAEWGAGALARLNGMFAFALWDAFEQELILARDRLGIKPLYYAFGARDEN
                     GAHANEAEASKFACGSFIFASEIKAILATGLVPRALDTEALDQYLTFLWTPDPHTVFR
                     GIRKLPPAHVLTWRAGEIEVREWWDVSFAEIEEGRSEAWWRERVLETLERVVEMEMVS
                     DVPLGAFLSGGVDSSSIVALMNRHSDGRRVSTYTVGIEQQDLRYDIIPDDARWARQVG
                     KLLDTDYHELMLQPDVCDLLPKLVYHMDEPVADPAVITSYLVSRAARETLTVLLSGVG
                     GDEVFAGYPRQLAMRIAGALDPVPLSFRRPLMRALSDVLPGGLPGRLTAPLRNAKKFA
                     RSAALDFEARYLGFGTYFTDAAKRRLYTDDLNEATRDADAYCEHRRHFARVRDAAPLN
                     RLLYVDLKTFLPCLNLAYTDKTSMAATLEVRVPFLNVELVELAARMPVNLKLRGLKRK
                     YILKRAAESLLPKEVVWRKKAGFGAPIRSWLRGALRPLVEDLLAEETVRRRGLFRPEE
                     VRRIIDANLSGREDYNLQVFQLLTLELWQRTFMD"
     gene            complement(11226..11612)
                     /locus_tag="VGB76_18485"
     CDS             complement(11226..11612)
                     /locus_tag="VGB76_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100942.1"
                     /translation="MSVADELPEDDEKLIVDQEWLAGAVESILKGDADAPETMARVFI
                     SFRDAIKRGLHGMNQLDEALLATIEIIYLQSRAHVAALKLYVLAQEGHLRVEDEPLNV
                     LGAAIARSRKGAERERVKARGRAQRA"
     gene            12772..13683
                     /locus_tag="VGB76_18490"
     CDS             12772..13683
                     /locus_tag="VGB76_18490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0100943.1"
                     /translation="MSDQGNNLKEQVRAFWQANPCGTKFTDAEPGSREFYERVAEHRY
                     ATEWHIPAAASFGRARNLRVLEIGCGLGTDGAQFARAGADYTGVDLTDAAVELARKCF
                     AVFNLKGDFRTADAEQLDFPDESFDLVYSHGVLHHTPDTARAVREVHRVLRPGGRAIV
                     MLYHRNSYNYRVNIGLLRRAGAHLLKTPAGLQLAHRLTGEPVESLGEHAARIKTDAES
                     YLAPGEFLNRNTDGAGNPLARVYSRREARALFKEFAEVETATHFLNKRWLPVVGQRLS
                     RPWEARLASRWGWHLWIYARKGASKTT"
     gene            13680..14642
                     /locus_tag="VGB76_18495"
     CDS             13680..14642
                     /locus_tag="VGB76_18495"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0100944.1"
                     /translation="MTDQRNILVTGGAGFIGSHLVERLLAERAWRVTVIDDFNDFYDP
                     TLKRRNVAPHLTRGDFQLVEADIRERAALERVFAATKFDCIVHLAARAGVRPSLSEPF
                     LYAETNITGTLNLLELARSTGVPQFVFGSSSSVYGINAKVPFSEDDPIRQTISPYAAT
                     KAAGEMLCHTYAHLHDMRCVALRFFTVYGARQRPDLAIHKFARLISTGKPIPVFGDGT
                     TRRDYTYVEDIIAGVRAAIDYRASSYEAINLGESRTVELRELISLLERELGREAVIDR
                     QPMQPGDVPQTFADISKARRLLGYNPQTQIEEGIRRFVAWFKEQ"
     gene            14735..15799
                     /locus_tag="VGB76_18500"
     CDS             14735..15799
                     /locus_tag="VGB76_18500"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0100945.1"
                     /translation="MKERLLEYLACPACEGELALASVAGRDESRGWTEILEGELACRG
                     CARRFAIRRGVPRFVEAGEIEGEKAATAENFGWQWQHFTQDDERYDEQFRGWLAPVAP
                     EFFKDKLVLEGGCGKGRHTQLAARWGAREVMGVDLSGAVETAFDATRDLPNAHILQAD
                     IYHLPLRRRTFDYAFSVGVLHHLPDPRAGFIALSTRVRAGGHLSAWVYGAENNEWITR
                     FVSPLRERVTSRMSRRMLLHLSKLPTALVYAATKLVYAPLNRSARAGALARHLFYNDY
                     LKSIADFGWREQHTIVFDHLVAPTAFYISRAEFEEWWREIEATDVRTGWHNQNSWRGF
                     GRIGQSVGRDDERQGEADGE"
     gene            15789..16580
                     /locus_tag="VGB76_18505"
     CDS             15789..16580
                     /locus_tag="VGB76_18505"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0100946.1"
                     /translation="MASEVKTNGLPDLPQEMERHTYAIMYEVEGSHWWFAGRRRILES
                     FVQRIVAELNTQGRAPRILDVGCGTGANLEMLAQFGEAEGVDVSEDALAFCRARGLTR
                     VTLGAAERLPYADDSFDLVTALDVVEHLDDDAGGLREMRRVLRPGGRALLFVPAFMWL
                     WGVQDDVSHHRRRYTLPQLTERVREAGFEIERATYANVTFFAPILAGRLLMRATGARP
                     ASENNINVSALNGVLGHIFGAERFWLRRLNFPFGVSAVCVARRVE"
     gene            16583..17623
                     /locus_tag="VGB76_18510"
     CDS             16583..17623
                     /locus_tag="VGB76_18510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0100947.1"
                     /translation="MRVYEEEETLKRRRVEVPRAAGTADDGGETPDISVFLPVFNEEP
                     NLRPLHAKMTDALDALGRTAEIIYVDDGSSDGSLEVLREIAAADARVRVIALRRNYGQ
                     TAAMSAGIDAARGRVLIPMDADLQNDPADIKRLLEKLDEGYDVVSGWRKDRQDKAITR
                     KFPSMLANRLISWIGGVPLHDYGCSLKAYRRDSLADVRLYGEMHRFIPIYASWAGARV
                     TELPVDHHARTMGVSKYGLSRTLKVIFDLVTIKFMASYQTKPIYVFGSFGMLAFAISF
                     IAGLWAVFLKLVEGVSFILTPLPVLAIVMFAVAIQFFLMGLLAEMQVRTYHESQAKTI
                     YAVRERIGFEKQ"
     gene            17708..19660
                     /gene="asnB"
                     /locus_tag="VGB76_18515"
     CDS             17708..19660
                     /gene="asnB"
                     /locus_tag="VGB76_18515"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY0100948.1"
                     /translation="MCGITGWAFMDARTPQAEGAAELLRSMCARMTHRGPDSEGLMTE
                     TGIALGMRRLAIIDLATGEQPALSEDGQVAVVLNGEIYNYRELRASLEDRGHSFRSAS
                     DTEVLPHLYEEYGTGMLAHLNGMFAFALWDARRRKLFIARDPFGEKPLYWGVFEGRLL
                     FASEPKVLLAHPSVRVRLNMDALRQYLSFDYVPAPLSIYEGIQKLPAAHALTLEDGRV
                     EVWPYWQLSYRKPERVPSEAEAAARVEELLADSVRLRLVSDVPLGVLLSGGVDSSTVA
                     ALAVRASAETVKTFSISFAEKSFDESAYARGVADFLHTDHHEERLNSNLAADLIGEIG
                     SWMDEPLSDPSLVPTYLLSRFTRQHVTVALGGDGGDEIFAGYQMYPAHRLARLYARVP
                     PAARRLFIESVVRRLPVKTKNLSLDFKARKFIEGMRYDDPVARHHVWFGSFTPEQQEL
                     LLTPAARGATSMDVYAGARALLAECDAEDTVERMQYLDTKLYLAEDILTKVDRTSMAV
                     SLEVRAPFLDRRVVEYVASLPASYKLHGSTSKYILKRAMRAHLPPFVTRRGKKGFGVP
                     VAEWLKGRLSPLARDLLSPARLSRHGLFDAAYVTRLQDEHERGAANHRKLLWTILIFE
                     LWHESFIETPRRMQPEVGERKSGVRG"
     gene            19768..20946
                     /locus_tag="VGB76_18520"
     CDS             19768..20946
                     /locus_tag="VGB76_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100949.1"
                     /translation="MIEVEQAYETLAAWCRARGYAGHDPFDGLNSRLFQSTPLRNSRA
                     ARLAWTQAFKRAPFSLRAAARVPAGRNSKGTALFALAALSRFRATRTSGAEREARELL
                     DALLSAQLAGWSGACWGYNFDWQGRAFFAPQGTPTIVPTAFAARALVEAAHSLADEKY
                     LRAAHSACEFILGDLKRSEERADEICFSYTPLDETRVFNASLLAGETLACVGALTKEE
                     ALVSAGVRAARYVVRRQQTSGAWAYGAADFQGWADNFHTAFILTSLARIMEASAEARA
                     EFAEPLRRGCEFWRENFFLADGWPKYYHDRQYPADAHAAGAAIVTLVELRDQMPCALA
                     LAERITRWTLENLRDGRRGFFYYQRRRFYTVRTPYMRWTQAWMLYALARQIEGMKDGG
                     "
     gene            21022..22050
                     /locus_tag="VGB76_18525"
     CDS             21022..22050
                     /locus_tag="VGB76_18525"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEY0100950.1"
                     /translation="MSRIWIDLANSPHVPFFVALREEFVARGHEVEVTAREFAQTVEL
                     AEQAGLRPTVMGEHGGRKLAGKAGNLLERARALRSWARGRRFELAVSHNSYSQIVAAR
                     ALRLRSVTLMDYEHQPANHLAFRLASRVIVPRVFPASALRRYGAPERKVQRYDGFKED
                     VYLADWQPAPGAFENLRDSLGVSERDVLIVVRPPARDALYHRFDNELFDALLEHLCAR
                     RDARIVLLARTDAQREEFDARHRSENVIMPENALDGATLIAASDLVVSAGGTMNREAA
                     ALGVPAATVYAGEWAAVDEALVREGRLTRIRTREDIGNLRVAKKDIATNARRAHGVRA
                     QVAEMILQ"
     gene            22236..>22540
                     /locus_tag="VGB76_18530"
     CDS             22236..>22540
                     /locus_tag="VGB76_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100951.1"
                     /translation="MRAERFDKLRIDASAVRVPGRAPKWALPSVKIALVAADALISVA
                     SFLAAFYLREGEHVITEFNAGRLNWSAEFAPYAAVLLFVVPVRVLAHAYYDLYKLRG"
BASE COUNT         4193 a         6831 c         7259 g         4257 t
ORIGIN      
        1 tagcgtttga agcccacacc cgtatcgcgt ccggcggtca cgccctcgtg atgaatgacg
       61 cgcgacatcg gttgatagac gactttgtag ccgagcgagc gcacgccgaa gcagatgtcc
      121 acgtcttcgt aataagcggg cgcgtagcgc gtgtcgaagc cgccgagccg ggcgaagaga
      181 tcgcggcgca cgaggagcga cgcgccggaa caatagtcaa cctcgcgcgc gaaattgtaa
      241 cgcctgtcgt ccggcgagcc gccccagccg taatgaaagg ccgcgccgtt ttgccagacg
      301 cccgcgccgg cttcctgcac gcgcccgtcc tcgtaaagaa acatcgaacc gaccgcgccg
      361 gttgatgcgt cgccctcgac agtctccagc aggtgcttca accagcccgg cagcaccacc
      421 gtatcgttat tcaagaagac gagataacgc ccgcgcgcca ccgccgcgcc ctgattgcac
      481 gcatcgacga agccgcgatt ctcctcgttg ttgaccactg tgacgagact ctgaaagtgc
      541 gacaacagtt tcgccgtctc atccgtcgaa gcgttgttga cgacgatcac ttccgtctcc
      601 gcgaaatcaa cttcgcgcag gagcgagcgc aggcattgga aggtgaaccc ggctttgttg
      661 tagacgggga tgacgattga cgtgcgcggc tgcctgtcgc tcgctcgcgg agcgttgtgc
      721 gcctcggcgg cggagagcgt ctcggtgaga aacagaaaat cctgcgcgct ccacacaggc
      781 aattgagcgg gcgcaaactc cggttcgtac gtgacgttcc agaccttgtc aacgtccaac
      841 ccgtgcttgg cggcaagacg caggatgcgc ctgccggagt tgtagccgaa ggctgcgaga
      901 cggacgaacg cgccgaccgt ggcgacgagc aactgcttcg acgccagttt cagagggaac
      961 ggcaagtttt cctgtagcca tttcttcatc gcgtgaaatt tttacgaacg ctcaagcgtg
     1021 cccgtcgtcg cgtggtgttc gcccggcggc gtcggcttga agcgcgtcgt cgtccagtcg
     1081 agcagcgggc gcacgacgcc ggggatagga ccaatgtagt cgccgcgcgc cgattcgccc
     1141 ggctgcgaac tctcgatcat ttgaaacgcg acgatgtttc gcgcctgcgc gtggagcgtc
     1201 gtcgtagggg tgtgcgacat gatggagacg tgcgcgcgca gcgtcccttc ggagaggaag
     1261 ttgccgggca gcgcgataac gctcgcgtag cggccaacct cgcgcggcgt gtgtcgccac
     1321 tcgtcgctcg tgtcgtgcga actgaaggcg cgcacgccct cttcgttaac cagcccgacg
     1381 tgcggcgtga ggatgtgccc ggcggcgagc acatcgtaag tgatctcgat gtggacgggg
     1441 cggcggatgt cgaaggcggt ggcgagcgcg cccgcttcgt cgagcacgcg cacgcggcgg
     1501 aggcgcacca agtcgttgcc gggcgcgtcc gccggagtct tccattcgca ctcggcggtg
     1561 atgttccaac tcgcgctcag gtaatcattg acgacggcct gcggttcgcc ctcgctcacg
     1621 atctgcccgg catggagcag gatgacgcga cggcacagac gcgtgacggc gggcatgctg
     1681 tgggagacga aaaagatggt gcgcccgtgc tggcgaatgg cgtgcatctt gtcgagacat
     1741 ttttgctgga aggccgcatc gcccacggcg agcacttcgt ccatgatcag gatttcgggt
     1801 tcgagatgcg cggcgacgga gaaggcgagg cgcacgtaca tgccggagga gtaacgcttg
     1861 acgggcgtgt cgatgaattt ttcgagttcg gagaaggcga cgatctcttc gaacttgcgt
     1921 tcgatctcgg cgcggcgcat gccgaggatc gcgccgctca aaaagacgtt ttcgcgcccc
     1981 gacaactccg cgtgaaaacc cgtcccgact tcgagcaggc tgccgacgcg cccgtacatc
     2041 tccacgcgcc ccgtcgtcgg cttcgtcacg cgcgagagga ttttcaggag cgtggatttg
     2101 cccgcgccgt ttctgccgat aacgccgatg agttcgcccg gttgcacgga gagattgatg
     2161 tcgcgtaacg cccagaagtc gcgccgccgt tcgttgacct gcccggcatc gccgccgccg
     2221 ttgcgccgga agggggtgag cagcgcgccg ccgaccatgt cgcggaaggt gagatagccg
     2281 ggatgcaggc cgccgagccg gtagcgtttg ctgacatttt cgatcttaac aatcggtttc
     2341 atcggaacag gttcaactca agccgggttc agatcaggtc tgcgaaagtc tcttcgatgc
     2401 ggcggaagag atagaaggag aacgcgagcg caacgagcgt cactatgatg gagagcagcg
     2461 tcgccgtgcg gtcgaacgtg ccgccggtga gcgcggcgcg aaagccttcg atgattccgg
     2521 tcagaggatt gagcgcgatc aggtgtcgcc atgctccggg cacgcgatgc gaaggataga
     2581 tgacgggcga ggcgaacatc cagaattgaa tgaggaaggg cacggcgtgg cgcaggtcgc
     2641 gatatttcac cgtgagcgcg gagatcaaca ttccgacggc gagcgcgagc atcacggcga
     2701 gcagcaggaa gagcggcaag agcgcgaggc cggaattgac cgcgaggccg taataaagga
     2761 tcagcccgac gaggatcaga ccggcgatgg cgaagtccac gagtcccgcg ccgaccgccg
     2821 ccgccgggat gaaggcgcgc ggaaagtaga ctttggtgat caggttcgtg ttgttgagca
     2881 ggctgttggt gctgttcgtg acggcgttgg agaagaacgt ccagatcaaa agccccgtgt
     2941 aagcgaagag cgggtagggg acgcgccccg tgtccagccc ggcgaggcga ttgaagacga
     3001 gcgtgaagag gagcatcgtg gcgagcggtt gaatgatcgc ccacgccgcg ccgagcgcgg
     3061 tctgtttgta gcgcaccttg atgtcgcgcc acgtcagaaa gtagagcagt tcgcgatacg
     3121 cccagaggtc gttgaacgca aactgcacgc ccgcgtcttc cgactcgatc aggatgagcg
     3181 ggcgatccgg aagcgtgatg acggcgtcgc tggctgcgtt ggctgtcgtg acagcgggag
     3241 acgatgcgtg tgtgagcggc gcggtggcgg cgggttgcgg tgtagacgcg attggttcgc
     3301 tggtgtgtga atccatcaaa ttcgagtcgc gtcagaaagc gttagaagca cgggtgtcgg
     3361 ctgaagcgcg cgcccggcaa tggccggagt tgaattaaga gtacgagtta gtctagacac
     3421 cggagggtga ggcgtcaaga atgtagggcg tagcgacggc gtaaagttcg cggatgaggg
     3481 tgcggagagt gtgcgtcaca cacttacaag agttgtcatg gacagctata tttgcagtga
     3541 gccgtagcgt gcgcctgcgc gtgcgcgcgc acggcctctg cgccagttga gcaagcggtg
     3601 cggcgtgagc aaaccggcga cgaacttgaa cgcttcgcct ctcgtccgcg cgccgcgcaa
     3661 atttctcgac gccagtttga gcgcttcgag tttttcgccc cgcctcataa attcctgcgc
     3721 gctgcgaaat cgtgcgagcg tctgaaactc cgcgagttcc tccgcgctca atccgagccg
     3781 ggcggccacg cgctgctgcg ccgcgagttt ctcccggagc atgaagcgca ggttacggct
     3841 cgcgttggaa ccgtgctcgc gccacgcgga gagcacgcgc ccgtcgaagg caaactcgcc
     3901 ttccgcgctc aggcgcaggt agagttcgta atcttccagg ccagccgctt cgttccaacg
     3961 gtgtgcttgc agcgcgtcgc ggcgatagac gaccgtgggg ctgagcggcg cgagagtttc
     4021 cagcagcatc agccggacgc tgccgtcgcg gtaacgcgcc cagtcagccg tgttatctat
     4081 gatgcgatct ccggcgtcaa tgctgtaagc gttcccgtag gcgaggacgg cgcgctcgcg
     4141 cgcttccaaa atctcgacgc gcgactgtag aaactgcgga aaccagagat cgtccgagcc
     4201 aagataagcg aaataacgac cgcgcgcggc gagcgcgaaa ccttcgttga gcgtcgcgca
     4261 caggccgcga ttctcgcgcg ccacgaacgc cgacgcggcg acgggcgagt cgcgcagcac
     4321 gcgctcgatg atccgcaccg agccgtctgt ggagccgtcg tctatcacga cgagttcgcg
     4381 cggcaagagc gtctgccgga agatggagcg caaggttttt tcgatgaacg cctcgtggtt
     4441 atatgacggc acgacgacgg agacggtcgg ctcgcccgtc gtctccgtcg cgccgggcgt
     4501 gttggctggc gcatttaaca ttcggtcttt atattaaaac aagtccggtc ttccatgaga
     4561 gtcttgcacc tactcgattc gctcaatcgc ggcggcgcgg aaacgctctg cctcgacgtg
     4621 tgccgcaacg cgcgccgcaa ctcgctcgat ctcaccttcg cggcgacggg cggcggcgaa
     4681 ctcgaagacg acttccggca gtccggcgca gacttcgttc gactcgcgcg cacgcttccg
     4741 ctcgatccga agttaatcaa gcaactgcgc gggttgatcg acgaacgcga gatcgaagtc
     4801 gtgcattgtc atcaggcggt cgaagccctg cacgcttacc tcgcgacgcg cggcggccag
     4861 cgcaaagggc ggcgcgtcaa gcgtgtgctg agctttcatc tctgcacggc tgacgcgaag
     4921 aatcgcctcg ccctgaagtt tctcgcgccg cgcatggacg ccaacgtcgc cgtcagccgc
     4981 gaactgctct cgtgcctcgg cgcggaaggc ggctttcaaa ttggcaggaa ctttcacgtc
     5041 gtctataacg gcgtggacgc gtcgcggctc gacgcgacgg gcggcggcgg cgacttgcgc
     5101 gcggagttgc gcctgacgaa gagagaagtg ctgttcggaa tggtcggtaa tttctacgcc
     5161 gaccgccgca aggatcagtt gaccgtgtgc cgcgcgttgc ccgaactgtt ccggcaggta
     5221 ccggaagcgc attttgtgtt tgcaggaggt tatgaagaga atgcttcggg gatttatcag
     5281 gagtgcgtga actattgccg cgaacaggga atcgcaggcc gcgtccattt tgcgggtaag
     5341 cgcgcggatg tcgcgtgcgt gctcgacgcg ctcgacatct tcgtcttctc ttcgcgcaaa
     5401 gattcgttcg gcgtcgcggt ggtcgaagcg atgctggcgg gcgtgcccgc gattgtctcg
     5461 gacatcggcg cgctgcttga agtaacgcgc ggcggcgagc atgcgtcgat ctttcgcacg
     5521 ggcgacgcgg atgatctggc gcgcaaaatg gtcgcggtcg cgtgcgaccc cgtgcgacag
     5581 cgcgaactcg gagaacaggc gcgccgttgg gcgaggcgcg agttcgggat cgagacgcac
     5641 atcgggaatt tgatcaaact atacgagtct ctcgcgggcg cggagtgatc tcgccccttt
     5701 gagttcagat tgtcattcgc ttattcgatg aacgggaaac gtccgcgcgt gcgcgtattg
     5761 atggggatgc ctgatcgtga gtctctgggt ggaccggcgg cctgcgagcc gcccttcgtc
     5821 gcggggttgc gcgaactcgg cgcggaggtc gtggaggaaa cttacgtcta cggcgagcgt
     5881 ccgggcggga cgaccttccg gcagcgcgtg ctgcgcgtgc ttgcgacggg gcggcgtctg
     5941 cgtcgcaccc tgcgcgcggg cgagttcgac atcgttcatc tgaactcatc tttcgacacg
     6001 atggctctca tgcgcgacgc cgcgacgctg cgcctcctgc tgcccgcgcg cgcgaaaatc
     6061 ttcatcaagt ttcacggctc cgacgccgcg ctcttcgaga cgaaaaactc cgccctgcgc
     6121 gcgctcgtgc gtttcgtcgt ggcgcacgcg gacggcatcg gcctgctgtc gtccgaggag
     6181 cgcgggaatt tcatccgcgc cggagttgat ggacgaaaac tcttcgtcgt gaaaaacgtg
     6241 gtgacgggcg cagaagatgc cggccatgca cgcacagagc acggcgcagg ttcgacgccg
     6301 cacgcgagcg cggcgcaggc gcagagtccg aacctgcgcg cgaagttggg cgtggacgaa
     6361 gagacgccgc tcctgctctt catcgcgcgc ttcatcccgg ccaaaggttt gacggacgtg
     6421 atcaacgctt gcgctatctt gcgcgatgca ggccgcgcgt tcgagttgtt gtgtctgggc
     6481 gacggcgagg cgcgcggggc ggcggaagcg gaaacggcgc gcctgaagtt gaatgagcgc
     6541 gtgcgcttct tcggctacgt accggaaacc gagacggcgg agttttacgc gggcagcacg
     6601 atgctcgtct ttcccaccta tcattacgaa ggtttcccga tggtcatttt caaatccgtc
     6661 gccgccgggc tgcccatcat cacgacgcgc atccgcgccg ccgcagacta tctacgcgag
     6721 ccggacaact gcctctgggt cgagccgcaa aacccgcgcc tgctcgcgga gaagatcacg
     6781 caacttctcg aacgggcgga gacgcgcgcc ctgatgcgtc gccacaatct cgaactcgcc
     6841 agccggttta ccgcacgcgt cgtagccccg gagtatctgg aaatatataa tcaactcacg
     6901 aacgcatgag acagaatcag caactcgtca acgcatgaaa cagattttgc agaactataa
     6961 gacgggcgaa gtctccgtcg ccgacgtgcc gccgcctatc gcgcagcgcg ggcgcgtgct
     7021 ggtacgcacc gccgcctcgc tcatcagcgc gggcacggag cgcatggccg tcgaactcgg
     7081 acgcaagagc ctgctcggca aagcgcgcga gcgtccagac ctcgtcaggc aagtgatcaa
     7141 gaaggcgcag cgtgaaggct tgctgaacac cttcaacgcc gtgcagacga aattgaacgc
     7201 cgcgaacgcg ctcggctaca gcgcggcagg catcgtgacg gacgtgggcg aagacgtgcc
     7261 gggattcagc gcgggcgacc gtgtggcctg cgcgggtgcg ggcttcgctt cacacgccga
     7321 agtgctctcc gtgccgaaga acttatgcgt gcgcctgccc gacgcggttg acttcgaagc
     7381 ggcctccttc ggcacgctcg gcgccatcgc gctgcaaggc gtccgtctgg ccgaactcac
     7441 gctcggcgaa tccgtcgtcg tcatcggcct cggcctcctg ggtcaactta cagtccaact
     7501 cctgaaggcg aacggctgcc gggtcttcgg catcgatctc gatccgtcga aagtggaact
     7561 cgcgcgcacg ctcggcgcgg acgacggctg cgtgtccggc ggcgagacgt tgcaggccgt
     7621 cttggattgg tcgcgcgggc gcggcgcgga tgcggtgctg atcacggcgg ccaccccgtc
     7681 cgatcaaccg gtagaactcg cgggtgagat ttcgcgtctt aaggggcgcg tcgtcgccgt
     7741 cggtctggtc ggcatgaacg tgccgcgcaa aatttattac gaacgcgaac tgacgctcaa
     7801 agtctcgatg tcgtacgggc cgggtcgcta cgatccggaa tacgaggagc gcggccacga
     7861 ttacccgttc gcctacgtgc gctggactga ggggcgcaac ctcgaagcct ttctcgacct
     7921 cgtcgcggcg ggcagcgtac ggatcgcgcc gctcatcacg cacaggtttc ccatcgaaga
     7981 cggcgagcgc gcctacaacc tgatcgcggg cgagacgaaa gagccttacc tcggcatcgt
     8041 gctcagctac gacaccgggc gcgaactcgt gcggagcatc gaactgcgcg ggcgcgacgg
     8101 caagaccaat gcgagcagcg cgcgtgcgcc gggcgcggcg tcggtgcatg tcggcatgat
     8161 cggcgcgggc agctacgcgc aaaagtttct gctgccgaac ttcaaagcaa acggcgcggc
     8221 cttccagtca attgcgacgg cttcgggcat caacgcgcgt ggcatcggcg agaagtacga
     8281 ctttgcgcgc gtcgccgcga gcgcggaaga ggttatcgca gaccccgacg caaacttgat
     8341 cgtcatcgcc acgcgccacg attcgcacgc ggaactcgcg cgccgcgcgc tcgcagcggg
     8401 gcgacacgtc ttcgtcgaaa aaccgctcgc cttgaacgac gaagagttgg acggcgtgct
     8461 tgaggctgcg tctgcatcga acggcgggcg gctgctggtc ggcttcaatc gacgcttctc
     8521 gccgcacgca cgcgaggccg cgcacgcttt cgccgcgcgc cgcgaaccgc tctccattct
     8581 ctaccgcgtc aacgccgggc gcatcgcgcg cacgagttgg attcaggatg agcgcgaggg
     8641 cggcgggcgc atcatcggcg aagtctgcca ctttatagac ttgatgcatt ttctgaccgg
     8701 agccgtcacc acgcgcgtct atgcagaagc gatagccagc cgcaacgagg agatcgtcga
     8761 cgaagattcc gtgttgatca cgctccggtt cagcgacggc tcaaacggac agatcgccta
     8821 tctcgcggaa ggcgaccgcg cgctcgccaa agagcgcata gagattttcg gcggcggcaa
     8881 aactttcgtc atcgcagact ttcgctccgc cgtgctctac gaaggcgggc gcgaacgccg
     8941 catgaaactg cgcgcgcagg acaaaggaca ggcggaagaa gtgcgcgccc tctgcgcgat
     9001 ggtcagggaa ggcacgcccg cgcccatcgc cctccaagac ctcgccgcca ccacgcgcgc
     9061 caccttccgc atacgcgaat ccctgcgcgc cggagaacca aaaacagtga cgagtgacag
     9121 gtgacaggtg acaggtgaca agttaaaacc agagacttgt atcgtgccgg atgcttgtat
     9181 tgttcttact cgtcactcgt cactcgtcac tgatctatca tgtgtggaat tgcgggacta
     9241 gttagccagc agccggagcg gcgcatcggc gcgatgttga agtctatcga gcatcgcggg
     9301 cgcgacgacg aaggtgtgtg gacttccgcc tcggtagatg gggaggggcg gcgggcgtgt
     9361 ctcggccaca ggcgtctgtc gatcatcgat ccgagcgagg ccgggcatca gccgatgcag
     9421 tatgccgacg gtcgctactc cgtcacgttc aacggcgaga tttacaacta ccgcgaactc
     9481 cgcagggaac tcgaaggacg caaccatcgc tttcgcaccg actgcgacac cgaagtgctg
     9541 ctcgcggcct acgccgaatg gggcgcgggc gcgctcgcgc gtctgaacgg catgttcgct
     9601 ttcgccctgt gggatgcttt cgagcaggag ttgatcctcg cgcgcgaccg cctcggcatc
     9661 aagccgctct attacgcgtt cggcgcgcgg gatgaaaacg gcgcgcacgc gaacgaggcg
     9721 gaggcttcga agttcgcgtg cggttcgttc atctttgcct ccgagatcaa agcgatcctt
     9781 gcgaccggac tcgtcccgcg cgcgctcgac acggaagccc tcgatcaata tctcaccttt
     9841 ctctggacgc ccgacccgca cacggtcttt cgcggcatac gcaaacttcc gcccgcgcac
     9901 gttttgacgt ggcgcgcggg cgagatagag gtgcgcgagt ggtgggacgt ttccttcgcg
     9961 gagatcgaag agggcagaag cgaagcgtgg tggcgcgagc gcgtgctgga aactctggaa
    10021 cgcgtcgtcg agatggagat ggtgtcggac gtgccgctcg gcgcgttcct gtcgggcggc
    10081 gtggattcct cttccatcgt cgcgctgatg aatcgccaca gcgacggtcg gcgcgtctcc
    10141 acttacaccg tcggcatcga gcagcaggac ttgcgctacg acatcatccc cgacgatgcg
    10201 cgctgggcgc ggcaggtcgg caaacttctg gacacggact atcacgagtt gatgctgcaa
    10261 ccggacgtgt gcgacctgtt gcccaaactc gtctatcaca tggacgaacc tgtcgccgac
    10321 ccggcggtca tcacgagtta tctcgtctcg cgtgcggcgc gcgagacgct cacggtgctg
    10381 ctctccggcg tcggcggcga cgaagtgttt gcgggctacc cgcgccaact cgccatgcgg
    10441 atcgcgggcg cgctcgaccc cgtgcccttg agttttcgcc gcccgctgat gcgcgcgctc
    10501 tcggacgtgc tgccgggcgg cctgcccggt cggctcaccg cgccgctgcg caacgccaag
    10561 aagtttgcac gcagcgccgc gctcgatttc gaggcgcgct atctaggctt cggcacctac
    10621 ttcacagacg ccgccaaacg ccgcctctac acggacgacc tgaacgaagc cacgcgcgac
    10681 gccgacgcct attgcgaaca ccggcgacac ttcgcgcgcg tgcgcgatgc cgcgccgctc
    10741 aatcgcctgc tctacgtcga tctgaaaact ttcctgccct gtctcaatct ggcctacacg
    10801 gacaagacct cgatggctgc gacgctcgaa gtgcgcgtgc cgttcctgaa cgtcgaactg
    10861 gtcgaactcg ccgcgcgcat gcccgtcaat ctgaaactgc gcgggttgaa acgaaagtat
    10921 attttgaaac gcgccgccga gagtttactg ccgaaggagg tggtctggcg caagaaggcg
    10981 ggcttcggcg cgcccatccg ctcgtggctg cgcggcgcgc tccgccctct ggtggaagat
    11041 ttattggcgg aagagactgt ccgccgtcgc ggcctcttcc gccccgaaga agttcgccgc
    11101 atcatcgacg cgaacctctc aggccgcgaa gactacaacc tacaggtctt ccaactgctc
    11161 acgctcgaac tctggcaacg cacgtttatg gactaaccgc cgcgcacgct tgaaagtctc
    11221 tccccctacg cccgctgcgc acgacctcgc gccttaaccc tctctcgctc cgcgcccttc
    11281 ctgctccttg cgatggccgc gccgagcacg ttcaacggct catcctcaac ccgcaagtgt
    11341 ccctcctgcg ccagcacgta caacttgagc gcggccacat gcgcgcgtga ttgcagatag
    11401 attatctcga ttgttgccag caaagcctcg tccaactgat tcatgccgtg caagcccctc
    11461 ttgatggcat ccctgaaact gatgaacacg cgcgccatcg tctccggcgc atccgcatcg
    11521 cctttcaaga ttgactcaac cgccccagcc aaccactcct gatcaacgat cagcttctcg
    11581 tcatcctccg gcaactcgtc tgcaacactc acggcaacct ccattccgac aatatcaaca
    11641 cggaataaaa tggctgatcc ctacgcgtgc tccgtctcat aaacgcgctt ataacatatt
    11701 cctcgcagcc ataatagacg gtactaatgc cgtatactcc acttatgcca tatacgaata
    11761 tgacatgaat aattgtagaa gtgggcacgt tgggcgggct gaaaggtggc aaggctcgcg
    11821 ctgaaaaatt atcgccggaa agaaggacag agatagctaa acgagcagcg caaattcgct
    11881 ggaataatgc ggatcgctcg ccaaagcaaa agcaaacttg agtctaagag gctcagattg
    11941 ttgatctagt tattgcgcgg attaaatcat cgtgctagtg tgtgcttgct atttgaattt
    12001 gttgggggcg ttttctatgg gtttctcctt tggcctccac aaagtttctg tccaataggg
    12061 ggcagaaatg accaaagccc tgtgttcgag cacagggctt tggagtagaa caaaggagaa
    12121 actaatgagt aagactgata accgttggac gtttcggtgg acactgagat tttgggtgtt
    12181 caccttgtca tgcgaaatca aaccgccata aggtgatttg attctaagag ggcgagctac
    12241 aactcgctct cttttctttt gcaaagatca actgtcagtt gacgatttgt aaactaccac
    12301 ttgagtcttg cgtaacgttc gttacgactt ataacatcgc atgttaagtg tatacacaat
    12361 agttgtcaag aagttcttga gtcgtaaaat catgtaaaca aacggcttaa cctataaaca
    12421 tattgtttat aacaacttgt agtttatgca atccaaatta atttggatca attaggttca
    12481 atatggtgat catatctcca ccatatgata acgatatgga ttgtatatac catgtctaga
    12541 cgttatctag acatggtata tagccggatc gtagtgcgcc gttcctgccc tgtcaaaaat
    12601 cttcttagag catacacaca cactgttacc ccactgtcgc atattccaat cgcttgtcgg
    12661 gcgcggaggc tgcgtgttaa tttcactgtt tcgcgtgggg agagtttttc cgtctgtagc
    12721 gtgcgagggg cgcgcgaggg gagataatat tcgcctgcgc tgcggctctc catgagtgat
    12781 cagggtaaca atttgaagga gcaggtgcgc gccttctggc aggcgaaccc atgcgggacg
    12841 aagtttacgg acgccgaacc ggggtcgcgt gaattctacg agcgcgtggc cgagcatcgc
    12901 tacgcgacgg aatggcacat ccctgcggcg gcgtcgttcg ggcgggcgcg gaatttgcgc
    12961 gtgctggaga tcgggtgcgg gttgggcacg gacggggcgc agtttgcccg cgcaggcgcg
    13021 gattatacgg gcgttgacct gacggacgca gcggtcgaac tggcgcgcaa gtgtttcgcc
    13081 gtcttcaatc tcaagggcga ctttcgcacg gctgacgccg agcaactcga ttttccagac
    13141 gagtctttcg atctcgtcta ttcgcacggc gtcctgcatc acacgccgga cacggcgcgg
    13201 gcggtgaggg aagtgcatcg cgtgctgcgc cccggtggcc gcgccatcgt catgctttat
    13261 catcgcaact cttacaacta ccgggtcaac atcggcctgc tgcgacgcgc cggggcgcac
    13321 ctgctcaaaa cgcctgcggg cttgcagttg gcgcatcgcc tgacgggcga gccggtcgag
    13381 tcgctcggcg agcacgcggc gcgcatcaag accgacgcgg agagttatct cgcgcccggc
    13441 gagtttttga atcgcaacac tgacggcgcg ggcaatcctc tggcgcgcgt ctattcgaga
    13501 cgagaggcgc gcgctctctt caaagagttc gcggaggtcg agacggcaac ccactttctg
    13561 aataaacgct ggctgcccgt cgtcggtcaa cgcctgtcgc gcccgtggga agcgcggctc
    13621 gcgtcgcgct ggggctggca cctgtggatt tacgcgcgca aaggagcgtc gaaaacaact
    13681 tgacggatca gcggaacatt ttagtgacgg gcggcgcggg cttcatcggc tcgcatctgg
    13741 tcgaacgcct gctcgcggaa cgcgcgtggc gcgtgacggt catagacgac ttcaacgact
    13801 tctacgaccc cacgctcaag cgcaggaacg tcgcgccgca tctcacgcgc ggagacttcc
    13861 aactcgtcga agcggacatc cgcgaacgcg ccgcgctgga gagagttttc gccgcgacga
    13921 agtttgattg catcgtgcat ctggcggcgc gtgcgggcgt gcgcccgtcg ctgagcgagc
    13981 cgttcctgta cgcggagacg aacatcacgg gcacgctcaa cctgctcgaa ctcgcgcgct
    14041 ctacgggcgt gccgcagttc gtcttcggct cctcttcctc cgtctacggc atcaacgcaa
    14101 aagtgccttt ctcggaagac gatccgattc gacagaccat cagcccctac gcggcgacga
    14161 aggcggcggg cgaaatgctc tgccacacct acgcgcacct gcacgacatg cgctgcgtgg
    14221 ccttgcgttt cttcaccgtc tacggcgcgc gccagcgtcc cgatctcgcc atacacaaat
    14281 ttgcgcggct catctccacg ggcaagccga tcccggtctt cggcgacggc acgacgcggc
    14341 gcgactacac ttacgtcgaa gacatcatcg cgggcgtgcg cgccgccatc gactaccgtg
    14401 cgagcagcta cgaagcgatc aacctcggcg aatcgcgcac ggtggagcta cgcgaactga
    14461 tctctctgct cgaacgagaa ctcggacgcg aagctgtgat agatcgccag cccatgcaac
    14521 ccggcgacgt gccgcagacc ttcgccgaca tctccaaagc gcgccgcctg ctcggctaca
    14581 acccgcagac acagatcgaa gaaggcatcc gccgcttcgt cgcctggttc aaagagcagt
    14641 gacgagtaac aggtgacgag agacgagtta aagcagcaat cttgtcttca aacttgtctt
    14701 ccgtcttgtc gcctgccccg tcagatcatc atttatgaaa gagcgtctgt tggaatatct
    14761 cgcgtgtcct gcgtgtgagg gcgagttggc gttggcctcc gtcgccgggc gggacgagtc
    14821 gcgcgggtgg acggagattt tggagggtga gttggcgtgt cgaggctgcg cgcgcaggtt
    14881 tgcgatcagg cgcggggtgc cgcgtttcgt cgaggcgggg gagattgaag gcgagaaggc
    14941 ggcgaccgcc gaaaacttcg gctggcagtg gcagcatttc acgcaggacg atgagcgtta
    15001 cgacgagcag tttcgcggct ggctcgcgcc cgtcgcgccg gagttcttta aagataaact
    15061 cgtgctcgaa ggcggttgcg ggaagggaag gcatacgcaa ctcgccgccc gctggggggc
    15121 gcgcgaggtg atgggcgttg acctgagcgg cgcggtcgag acggccttcg acgccacgcg
    15181 cgacctgccg aacgcgcaca tcctgcaagc cgacatctat catctgccgc tcaggcgtcg
    15241 cacgttcgat tacgccttct cggtcggcgt gctgcatcac ctgcccgacc cgcgcgcggg
    15301 cttcattgcg ctctcgacgc gcgtgcgtgc gggcggtcat ctttcggcgt gggtctacgg
    15361 cgcggagaac aacgaatgga tcacgcgctt cgtctctccg ctgcgcgagc gcgtcacttc
    15421 gcgcatgagc cgccgcatgc tgctgcacct ctccaagttg ccgaccgcgc tcgtctatgc
    15481 cgcgacgaaa ctcgtctatg cgcctttgaa ccggagcgca cgcgccgggg cactcgcgcg
    15541 ccatttgttt tacaatgact acctgaagtc aatcgcggat ttcggctggc gcgagcagca
    15601 caccatcgtc ttcgatcatc tggtcgcgcc gacggccttc tacatctcgc gcgcggagtt
    15661 tgaagagtgg tggcgtgaga tcgaggcgac ggacgtgcgg acgggctggc acaatcaaaa
    15721 cagttggcgc ggcttcggac gaatcggcca aagcgttggc cgcgacgacg agcgacaggg
    15781 cgaagcagat ggcgagtgaa gttaaaacga acggcctgcc cgatttgccg caggagatgg
    15841 agcggcacac ttatgcgatc atgtacgaag tcgaaggatc gcactggtgg tttgccggtc
    15901 gccgccgcat cctcgaaagt tttgtgcaaa ggatcgtcgc cgaactgaat acacagggac
    15961 gcgcgccgcg cattctcgac gtgggttgcg gcacgggcgc gaacttggag atgctggcgc
    16021 agtttggcga ggcggaaggc gtagacgtgt cggaagacgc gctcgccttc tgtcgcgcac
    16081 gcggtttgac gcgcgtgacg ctcggcgcgg cggaacgcct cccttacgcg gacgactcgt
    16141 tcgatctcgt caccgcgctc gacgtggtgg agcatctgga cgacgacgcg ggcggcctcc
    16201 gtgagatgcg gcgcgtgctg cgtcccggcg gtcgcgcgct gctgttcgtc ccggccttca
    16261 tgtggctgtg gggcgtgcag gacgacgtga gccatcaccg caggcgttac actctgccgc
    16321 aactgacgga gcgcgtgcgt gaggccgggt tcgagatcga gcgcgcgact tatgcgaacg
    16381 tcacgttctt cgcgcccatc cttgcgggac gcctgctcat gcgcgcgacg ggcgcgcgtc
    16441 ccgcgtcgga aaacaacatc aacgtgtcgg ccttgaacgg agtgctcggc cacatcttcg
    16501 gcgcggagcg tttctggctg cgccgtctga attttccctt cggcgtctcc gccgtgtgcg
    16561 tggcgcggag ggtggagtag cgatgagagt ttacgaagaa gaagagactt tgaagcggcg
    16621 gcgcgtggaa gtgccgcgcg cggcgggcac ggcggacgac ggcggcgaga cgccggacat
    16681 ctccgtcttt ctgcccgtct tcaacgaaga gccgaacctg cgcccgctcc acgcgaagat
    16741 gacggacgcg ctcgacgcgc tcgggcgcac ggcggagatc atttacgtgg acgacggttc
    16801 gagcgacggc agcctcgaag tgttgcgcga gatcgcggct gcggacgcgc gcgtgcgggt
    16861 catcgccctg cgacgcaatt acgggcagac cgccgcgatg agcgcgggga ttgatgcggc
    16921 gcgcgggcgc gtgttgattc cgatggacgc cgacctgcaa aacgatccgg cggacattaa
    16981 acgcctgctg gagaagttgg acgagggtta cgacgtggtt tcgggctggc gcaaagaccg
    17041 gcaggacaag gccatcacgc ggaagtttcc ttcgatgctg gcgaaccgcc tgatctcctg
    17101 gatcggcggc gtgcccctgc acgactacgg gtgctccctg aaagcctatc gccgcgattc
    17161 gctcgcggac gtgcggctct acggcgagat gcaccgcttc atcccgatct acgcttcgtg
    17221 ggcgggcgca cgcgtcacgg agttgcctgt agaccatcac gcgcgcacga tgggcgtctc
    17281 gaagtacggc ctgtcgcgca cgctcaaggt catcttcgat ctcgtcacga tcaagttcat
    17341 ggcgtcgtac cagacgaaac cgatctacgt cttcggctcg ttcggcatgc tggccttcgc
    17401 gatctctttc atcgcgggac tgtgggcggt ctttctcaag ctcgtcgagg gcgtctcttt
    17461 catcctgacg ccgctgcccg tgctcgccat cgtcatgttc gccgtcgcca tacagttttt
    17521 cctgatggga ctcctcgccg agatgcaggt gcgcacctat cacgaatcgc aggccaagac
    17581 catctacgcc gtgcgcgaac gaatcggatt tgaaaaacag tgacgagtga caagttaaat
    17641 caagaacttg tctttaaact tgtcgcttgt tcctaactcg tcactcgtca ctcgtcactg
    17701 ttctcttatg tgtggaatca ccggatgggc gtttatggat gcgcggacgc cgcaggcgga
    17761 gggcgcggcg gagttgctgc gttcgatgtg tgcgcggatg acgcatcgcg ggcctgactc
    17821 ggaagggttg atgaccgaga cgggcatcgc gctcggcatg cgacggctcg ccattattga
    17881 tctggcgacg ggcgagcagc ccgccctgag cgaagacggg caggtggccg tcgtcttaaa
    17941 cggcgagatc tataattatc gtgagttgcg cgccagtctg gaagatcgcg gtcattcgtt
    18001 tagaagcgcg tcggatacgg aggtgctgcc gcatctctac gaagagtacg gcacggggat
    18061 gctcgcgcac ctgaacggca tgttcgcctt cgccctgtgg gacgcgcgcc gtcgcaagtt
    18121 gttcatcgcg cgcgacccgt tcggcgagaa gccgctttat tggggcgtgt tcgaggggcg
    18181 tctgttgttc gcgtcggagc cgaaagttct gctggcgcac cccagcgtgc gcgtgcgtct
    18241 gaacatggac gcgctccggc aatatctctc tttcgattac gttcccgcgc cgctctcgat
    18301 ctacgagggc atccagaaac ttcccgccgc gcacgcgctc acgctcgaag acgggcgcgt
    18361 cgaagtctgg ccttactggc aactcagtta tcgcaagccg gaacgcgtgc cgagcgaagc
    18421 ggaggcggcg gcgcgcgtcg aagagttgtt ggccgactcg gtgcgcctgc gtctcgtgtc
    18481 cgacgtgccg ctcggagtgc tcttgagcgg cggcgtggat tcttccacgg tcgccgcgct
    18541 cgccgtgcgc gcctcggctg agacggtcaa gactttctcc atctctttcg ccgaaaaatc
    18601 tttcgacgaa tcggcctacg cgcgcggcgt cgcagacttc ctgcacacgg atcatcacga
    18661 ggaacgcctg aactcgaacc tcgccgcaga cctcatcggc gagatcggct cctggatgga
    18721 cgaacccctg agcgaccctt cgctcgtgcc cacttacctg ctctcgcgct tcacgcgcca
    18781 gcacgtgacg gtcgcgctcg gcggcgacgg cggcgacgag attttcgcgg gctaccagat
    18841 gtatcccgcg catagattag cgcggctcta tgcgcgcgtg ccgcccgctg cgcgtcgcct
    18901 gttcatcgaa tcagtcgtgc ggcgtctgcc ggtcaagacg aagaatctct cgctcgattt
    18961 caaggcgcgc aagtttatcg aaggcatgcg ctacgacgat ccggtggcgc gtcatcacgt
    19021 ctggttcgga tcgtttacgc cggagcagca ggaacttctg ctcacgcccg ccgcgcgggg
    19081 cgcaaccagc atggatgtct acgcgggcgc gcgcgctctg ctcgcagagt gcgacgcgga
    19141 ggacacggtc gagcggatgc agtatctcga cacgaagctc tatctcgccg aagatatttt
    19201 gacgaaggtt gaccggacga gcatggccgt ctcgctcgaa gtgcgcgcgc cgtttctcga
    19261 ccgccgcgtg gtggaatacg tcgcgtcgct cccggccagt tacaaactgc acggcagcac
    19321 ctcgaagtat attttgaagc gcgcgatgcg ggcgcacctg ccgccgtttg tcacgcgacg
    19381 cggcaagaaa ggcttcggcg tgcccgtcgc cgaatggctc aaggggcgtc tcagcccgct
    19441 cgcgcgcgat ctgctgtcgc ccgcgcgcct cagtcggcac ggcctgttcg acgcggccta
    19501 cgtcacgcgc ctgcaagacg aacatgaacg cggcgcggcc aaccatcgca aactgctctg
    19561 gacgatcctc atcttcgaac tctggcatga gagttttatc gagacgccgc gccggatgca
    19621 gccggaggtc ggagagcgga agtcgggggt gagaggttaa aggtgagagg ccggttgaga
    19681 ggcgcgcaga ggcgagaggc cgggagccgg ttaaatcagc ctgcgctttg aactgactcc
    19741 ggcctctcaa ctccggcctc ttctattatg attgaagtcg agcaggcgta tgagactctc
    19801 gcagcgtggt gccgcgcgcg cggctacgcc ggacacgatc ccttcgacgg tttgaacagc
    19861 cgcctgttcc agtccacgcc tttgcggaac tcgcgcgccg cgcgtctcgc ttggacgcag
    19921 gcgttcaagc gcgcgccgtt cagtctgcgt gcggcggcgc gcgtgcctgc cgggcgcaac
    19981 tcgaaaggca cggctttgtt cgcgctcgcc gcgctctcgc gttttcgcgc gacgcgaaca
    20041 tccggggcgg agagggaagc gcgcgagttg ttggacgcgc tgctctcggc gcaacttgcg
    20101 ggatggagcg gcgcgtgctg gggctacaac ttcgactggc aggggcgcgc ttttttcgcg
    20161 ccgcaaggga cgccgaccat cgtgcccacg gccttcgccg cgcgcgctct cgtggaggcg
    20221 gcgcacagtc tcgccgatga aaaatacctg cgcgcggcgc atagcgcgtg cgagtttatt
    20281 ctgggcgacc tcaaacggag cgaagagcgc gcggacgaaa tttgtttcag ctatacgccc
    20341 cttgatgaaa cgcgcgtctt caacgcgagc ttgctggcgg gggagacgct ggcctgtgtc
    20401 ggcgcgttga cgaaggaaga agcgttggtg tccgcagggg tgcgtgcggc gcgttacgtc
    20461 gtcaggcggc agcaaacgtc gggcgcgtgg gcttacggcg cggcggactt tcagggttgg
    20521 gctgacaatt ttcacacggc gttcattctc acctcgctcg cgcgcatcat ggaggcgagc
    20581 gcggaggcgc gtgcggaatt tgcggagccg ctcaggcgcg gctgcgagtt ctggcgggag
    20641 aacttctttc ttgcggacgg ttggccgaaa tactaccatg acaggcagta cccggcagac
    20701 gcacacgcgg cgggcgcagc catcgtcacc ttagtcgaac tgagagatca gatgccgtgc
    20761 gcgctcgcgc tcgccgaacg catcacgcgc tggacgctcg aaaatttacg cgacgggcgg
    20821 cgcggcttct tctactacca acggcgtcgc ttctacaccg tccgcacgcc ctacatgcgc
    20881 tggacgcaag cgtggatgtt gtacgcgctg gcgagacaga tcgaagggat gaaggatggg
    20941 ggatgaggga tgtataagaa aacaactcga cggagttgag cattcctttc atccctcatc
    21001 cctccgccct catctctttt tatgtcgcgt atctggatcg atctcgccaa ctcgccgcac
    21061 gtgccgttct tcgttgcgtt gcgggaggag tttgtggcgc gcgggcacga ggtggaagtg
    21121 acggcgcgag agtttgcgca gacggtcgag ttggccgagc aggcaggctt gaggccgacg
    21181 gttatgggcg agcatggcgg gcgcaagctg gcgggcaagg cgggcaacct gttggagcgt
    21241 gcgcgcgctt tgaggagttg ggcgcgcggg cgtcgcttcg agttggccgt cagtcataat
    21301 tcatactcgc agatcgtggc ggcgcgcgcc cttcgtttgc gaagcgtgac gctgatggat
    21361 tatgaacacc agcccgcgaa tcacctggcg tttcgactgg cctcccgcgt catcgtgccg
    21421 cgcgtgtttc ccgcctccgc gctccggcgt tacggcgcgc cggagcgcaa ggtgcagcgt
    21481 tacgacggct tcaaggagga cgtttatctg gcggactggc agcccgcgcc cggcgcgttt
    21541 gaaaacttac gtgactcgct cggcgtgagt gaacgagacg tgctgattgt ggtgcgcccg
    21601 cccgcgcgcg atgcgctcta tcatcgcttt gacaacgaac tgttcgacgc gctgctcgaa
    21661 cacttatgcg cccggcgcga cgcgcgcatc gtcctgctgg cgcgcacgga cgcgcagcgc
    21721 gaggagtttg acgcgcgcca ccggagcgag aatgtcatca tgcccgaaaa cgctctcgac
    21781 ggggcaacgc tcatcgccgc ctcagacctc gtcgtcagcg cgggcggcac gatgaaccgc
    21841 gaagccgccg cgctcggcgt gcccgccgcc acggtctatg ccggagaatg ggcggcggtg
    21901 gacgaggcgc tcgtgcgcga aggcaggctc acacgcatcc gcacgcgcga ggacataggg
    21961 aacctgcgag tcgccaaaaa ggatatcgcg acgaacgcac gccgcgccca cggcgtccgc
    22021 gcccaagtgg ccgaaatgat attgcagtga cgagggaggg gttaaaacaa gagacgtaaa
    22081 tcgtaagccc tgaaatcgta agccgtgaaa acgcaaatcg tgaaatcgta agccgtgaaa
    22141 tcgtgaatag agaaaaactg ctttcgtcta tttacgattt acgattcacg atttactgct
    22201 ttaatctcgt cactcgtcac ttgtcactta aaaagatgcg ggctgagcgt ttcgataaat
    22261 tgagaattga tgccagcgcg gtgcgtgtgc cggggcgcgc gccgaagtgg gctttgccgt
    22321 ctgtgaagat cgcgctcgtg gcggcggatg cgctcatctc ggtggcgtcg tttctggcgg
    22381 ctttctatct gcgcgagggc gagcacgtca tcacggagtt caatgcgggg cggctcaatt
    22441 ggagcgcgga gtttgcgccg tacgcggcgg tgctcctgtt cgtcgtgccc gtgcgcgtgc
    22501 tggcacacgc ttattacgat ctttacaaac tgcgcggcga
//
LOCUS       DASWNS010000541         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_461038, whole genome shotgun sequence.
ACCESSION   DASWNS010000541 DASWNS010000000
VERSION     DASWNS010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461038"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VGB76_18535"
     CDS             <1..299
                     /locus_tag="VGB76_18535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0100952.1"
                     /translation="PLVTITVPFTMTGSLALSLVPERPPALFTTTLSGQGTATLLLSS
                     YFTESHGWLHDFQGVTFNFAPAAPAAVPEPATLILFGTGLTAMAARYRRRRRKD"
     gene            complement(505..1242)
                     /locus_tag="VGB76_18540"
     CDS             complement(505..1242)
                     /locus_tag="VGB76_18540"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0100953.1"
                     /translation="MEEAGSKPADTPRRRLLRRIAASRLWRAQVRYSVVLVAFAVLAF
                     YARANAYFAWDRAVALWLQSWRTDALNLLMFAVSYIGYGIVPYALAAVTFLVFLFFGL
                     RSEAFGLLLSTAGSGILNRAFKMLIARPRPAAPDVNVFRLHESQSFPSGHVTFYVCYF
                     GFLFFVAYALLPKRTLARRLALTVNASMVALVGLSRVYLGAHWPSDTIGAYFLGGLWL
                     AFSLYMYRRWKEGSTFHAKEPDVEAGT"
     gene            1387..2415
                     /locus_tag="VGB76_18545"
     CDS             1387..2415
                     /locus_tag="VGB76_18545"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0100954.1"
                     /translation="MLRVEYKEGLYLPDLDLWLDADAPREHCVISHAHADHIAEHRSI
                     VSTPETARIFQHRRGETEVTTQVYGERRDYGRYALTFYPAGHCLGSSQILVEAGGERL
                     VYTGDIKLRPNVAAESAVIIPCDTLVMESTFGDPHYRFPPDVATFERLYAGIDRAFSD
                     ERVPVVLAYALGKSQEALELLLRRGYRVTLHGSVWNVAEIYRECGVEFSGRYEKYDRE
                     HLKGRVLIAPPGCRKQPMLLNIERRYLIMLTGWALNRSAPYMYKGVDLILPLSDHADY
                     DDLVRLARETGAARIITMHGEPKFAAHLRELGLNAEHLAHHPSAPAKTKKRAPKKAAN
                     AVNRSLFD"
     gene            complement(2584..>2795)
                     /locus_tag="VGB76_18550"
     CDS             complement(2584..>2795)
                     /locus_tag="VGB76_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100955.1"
                     /translation="KPPDLNALLNAFTRALSSPVANEQYMVVTMRLPVNVLQILTDVD
                     PNLERAITRICNDCGEQVKALTAKP"
BASE COUNT          548 a          860 c          878 g          509 t
ORIGIN      
        1 cgccgctcgt cacgatcacg gtacctttta ccatgaccgg cagcctcgcc ctgtcgctcg
       61 tccccgaacg accgccagcc ctcttcacca cgacgctcag cgggcaggga accgcgaccc
      121 tgcttttgag cagctacttc accgaatcac acggctggct ccacgatttt cagggcgtca
      181 ccttcaactt cgcgcccgcc gcgcccgccg ccgttcccga accggcgacc ctcatcctgt
      241 tcggcacggg gctgaccgcc atggccgcgc gctaccgtcg tcgccgccgc aaagattaaa
      301 ccggacgtaa gggtcgcatg agagacggga tgtgcgatga ggcgggatgt gtgatgtaga
      361 tgcgtcgtag cgcggtgaga aattttcctg acggcttcgg cgggcgaaac aaactctgtt
      421 ccaactccct gtaagcgcac cctcgcgcgt cgccgcctgt cccgacgctg aatcttaaat
      481 ccggcaaata aactgcacgc ctgtctacgt cccggcctca acgtccggct ctttcgcatg
      541 gaaggtcgag ccttctttcc agcggcgata catgtagagg gagaaggcga gccagaggcc
      601 gccgaggaag tatgcgccga tggtgtcgct cggccagtgt gcgccgagat agacgcgcga
      661 gaggccgacg agggcgacca tcgaggcgtt gacggtgagc gcaaggcggc gtgcgagcgt
      721 acgtttcggc agcagcgcat aggcgacgaa gaagaggaag ccgaaatagc agacgtagaa
      781 ggtgacgtgt cccgaaggaa agctctggct ctcgtgcagg cgaaagacgt tcacgtcagg
      841 ggcggcgggg cgcgggcgcg cgatgagcat tttgaaggcg cgattcagga tgccgctgcc
      901 cgccgtgctc aacagcagcc cgaaggcttc gctgcgcagg ccgaagaaca gaaagacgag
      961 aaaggtaacg gcggcgagcg cgtaaggcac gatgccgtag ccgatgtaag agacggcgaa
     1021 catcagcagg ttgagcgcgt ccgtgcgcca cgattgcagc caaagggcga cggctctgtc
     1081 ccatgcgaag taggcgttgg cgcgtgcgta aaaggcgagg acggcgaagg cgacgagcac
     1141 cacggagtag cgcacctgcg cgcgccacag gcgagaggcg gcgatgcgtc gcaacaggcg
     1201 acggcgcggt gtgtctgcgg gcttgcttcc ggcttcttcc atgttcgtta tttcggacga
     1261 cagcgaaagt tcggttaacg cctgctcgcg agtgttttat cataccttca cttcaaacca
     1321 cgataagatg acggcacgca tctaactttc gccagcgtcg ctgcgataaa gagaaggggg
     1381 ttcgccgtgc tcagagttga atacaaggaa gggttgtacc tgcccgatct cgacctgtgg
     1441 ctcgacgccg acgcgccgcg cgagcattgc gtcatctcgc acgcacatgc ggatcacatc
     1501 gcggagcatc gttcgattgt ctccacgccg gagacggcac gcatctttca acaccgtcgc
     1561 ggcgagacgg aagtgacgac gcaggtgtac ggcgagcgac gcgactacgg gcgttatgcg
     1621 ctgactttct acccggcggg gcattgcctc ggctcgtcgc agattctcgt cgaagccggg
     1681 ggcgagcggc tggtctatac gggcgacatc aagttgcgcc cgaacgtcgc ggcggagtcg
     1741 gcggtcatca tcccctgcga cacgctcgtc atggagtcta ctttcggcga cccgcactat
     1801 cgcttcccgc ccgacgtggc gaccttcgaa cgcctctacg ccggaattga tcgcgccttc
     1861 tcggacgaac gcgtgcccgt cgtgctcgct tacgcgttgg gcaagagtca ggaggctctg
     1921 gagttgcttt tgcggcgcgg ctatcgcgtg acgctgcacg ggtcggtgtg gaacgtggcg
     1981 gagatttaca gggagtgcgg cgtggagttt tcggggcgtt atgaaaagta cgaccgcgag
     2041 cacttgaagg ggcgcgtgct catcgcgccg ccgggttgcc gcaagcagcc gatgctcctc
     2101 aacatcgaac gccgctacct gatcatgctc acgggctggg ctttgaaccg cagcgcgccc
     2161 tacatgtata agggcgtcga tctcatcctc ccgctctccg atcatgcaga ctacgacgat
     2221 ctcgtgcgcc tcgcacgcga gacgggcgca gcgcgaatca tcaccatgca cggcgagccg
     2281 aagtttgccg cgcacctgcg cgaactcggc ttaaacgcgg agcacctcgc gcaccatccg
     2341 agcgcgcccg ccaaaacgaa aaagcgcgcg cccaaaaaag ccgccaacgc cgtcaaccgt
     2401 tccctcttcg actgaaaaag tcccgtcagg gaggtttgaa gttttcgctg ccaccttcaa
     2461 acgtccctga cgggacacgc cttttcaact tcgcacctta tcccggacga tcaacagccg
     2521 cgctcttttc aaaccttcct gacgcgactt tcagacgacc tctcaggctt cctccgcata
     2581 aatttacggc ttggcggtca acgccttcac ttgctcgccg cagtcgttgc agatgcgcgt
     2641 gatggcgcgt tcgaggttgg ggtcaacgtc tgtcagtatt tgcaggacgt tgacgggcag
     2701 gcgcatcgtg acgaccatgt attgctcgtt ggcgacggga ctggagagcg cgcgcgtgaa
     2761 agcattgagc agcgcattga ggtcgggcgg cttcg
//
LOCUS       DASWNS010000542        10966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_436931, whole genome shotgun sequence.
ACCESSION   DASWNS010000542 DASWNS010000000
VERSION     DASWNS010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10966
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436931"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VGB76_18555"
     CDS             complement(<1..972)
                     /locus_tag="VGB76_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100956.1"
                     /translation="MNGFFLRPAFPFARNTQASTGTTFVITNNNDSGAGSLRDAITAA
                     NASPGTDTIAFNLPPGAQKIEVGLAFPLPLPDITDPLIIDGTTQPGYDGKPLVVLSGA
                     KLFKADSHGLTILSGSTVVKSLVINGFVSAGIHMKEKGGNVVQNCYIGTDPGGTQHVG
                     NNAGVYLLGSSGNLIGGAAAGDGNVLAGNRGPDIHLGIGSHSNQVSGNMIGTDVSGSI
                     ALKQGSQTFSIFSGSNNVIGGNTPGARNVIIGGLTIDGDTPYGASTGNHVKGNYIGLN
                     RAGTAALNPVGSGVSIGSRSTGNLIGGTTPGERNIISGNGTPGVVISG"
     gene            1664..2173
                     /locus_tag="VGB76_18560"
     CDS             1664..2173
                     /locus_tag="VGB76_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100957.1"
                     /translation="MKRKIAAGSVALVALVALSLVVASAAAKADFSGKWSLDKTKSEG
                     LPPNIEQTMTVTQTGDKIELETKVTTPQGERVVKDSYTVDGKEVDFTPQGPQGAIGKG
                     KRTAKWSADGMGLEVSETATLDGPNGADEVSATRKWTLAPDSKTLTIELTFNGEQGTQ
                     KTKRVFVKQ"
     gene            complement(2272..6255)
                     /locus_tag="VGB76_18565"
     CDS             complement(2272..6255)
                     /locus_tag="VGB76_18565"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEY0100958.1"
                     /translation="MSQDSATTENISTERTLGAHRLAQYVSRNRCERYLRLALFPGEA
                     KTLAARYRVGFENLSPLLSESGQAFEREQVAELATRAALHNLQNTSAAAFIAALKSQA
                     AGSRSFYYQARLEGRIGGCASEGYPDLVEIVRHAGDRDDTVDVTVIDIKASRRETVGF
                     RLQVAFYARLLQAALREAGLKPATLKGAIAARGSELSPDRIERFDLSLYLDEIEQLVA
                     APDSDVARAAARSFTDASYHLGAHCDGCPYNALCFIDTAEREDLSLVPTITATEKSAL
                     ISMGMTTARELFSLMNYGHGAMLPAPDKASDFARIGGRWPLGARLPVLAQRARAALKS
                     LGSNVEAKRYMLGADWGSLPDEGQYPNLIKVYVDAQRDHLEDKVYLLSALVAGPAGAS
                     EIVEHTPTKPDTESERALLVGWIARLLPAIAEAADSTSAPIHVYLYDRRGQRSLLDAL
                     ARHFEALCAIPAFYDLLTSTPALTQGMISFLAEEVGERLNLGAVCQNLYEVAREMNFE
                     WRDGEREFLKTFRARIFDNKRLFARDAASGEFVAATAEERAPNTARVESAARFGTEIP
                     LEYAYAAWGLLRESPEMQERERTQIRGFMGTSLEDLCALAAERCRALRFIEESFQYKN
                     RQVEKQPLALERLDQTELDTNAITLGRALEDFLYLEHHAKMQELLLHLSLPPEQRAAG
                     GRTLVVRCDSYEKSERGEERAEFSFARLDGELLTGTDAAEMCALRFREGDWVLLNPAT
                     DETGKFLPARKLVRGRLAVVESLDEGRVRLRLLAMSFKNSEFRYPHWLFKPEAGAVCT
                     IDEMADDLNADKFLDACRHAASNHLYGWLADTEEGKRQRAIRPKRLRDAKEIAALASR
                     AQAPYGLTEAQARVVGDYLTERVLVLQGPPGTGKSHTLGFAVLARALALATPARPFRV
                     AVAAKTHAATSIALASIARRARQLLKAAPDDPRLAPLEGLRVAKISNDAGEELAEGVV
                     RLFADGGSELSAVEQWAMLLEEPLLVVGGTPGGIYNLVKKGASKGRAIDWSQELFDLV
                     VVDEASQMGIAEAMTAAAFLRGDGQFIAIGDHRQMPPILAHAWDRESRRDLKHARPHL
                     SIFETLRAHGFKSEALDQSFRIPAEIAAFLQRHIYAADDINFRSDRRERMMPGDGLAG
                     WLGAAFEAEHSLVVVEHDECGSQQSNEFEASLVEELAGVACEKLGCGGEGGIGVVVPH
                     RAQKALLRARLPQLANSIDTVERFQGGERELIILSATVSDREFAQLESGFLLEPRRLT
                     VAISRPKRKLIVIASRAVFDLIPADLDEYERGALWKYLRHECSRKTLWEGELRGQRVR
                     VRTLCES"
     gene            complement(6342..7052)
                     /locus_tag="VGB76_18570"
     CDS             complement(6342..7052)
                     /locus_tag="VGB76_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100959.1"
                     /translation="MEAKDNIRLDTPPEQIASQPTSPLRQLQCAECLEAVAAEVAVAS
                     RRGETLCAGCARNYYVACANCDGLIPQDEARTHNDAVYCFDCEAQALKAKAGDVPGES
                     EVETLVSEYIALHGEEKRIKERLDEIKEQLKAAASVRERVAGAVTLSGAEGAVKCSFK
                     ASLKCIPEKVSALEQQLEPDKFAALFERKVSYSPVREQLEKLLSDDDATGANLRESIL
                     AAVERTETPTLTVVRPKK"
     gene            7364..7576
                     /locus_tag="VGB76_18575"
     CDS             7364..7576
                     /locus_tag="VGB76_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100960.1"
                     /translation="MNRTQFHSIAARGQRASGRTRAEQFGEGFSVAAVLCPGSLPTYL
                     PGGEVMRKGASSTINLWNSDRRGNLI"
     gene            7869..7951
                     /locus_tag="VGB76_18580"
     tRNA            7869..7951
                     /locus_tag="VGB76_18580"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7902..7904,aa:Tyr,seq:gta)
     gene            7969..8040
                     /locus_tag="VGB76_18585"
     tRNA            7969..8040
                     /locus_tag="VGB76_18585"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8002..8004,aa:Trp,seq:cca)
     gene            8051..8137
                     /locus_tag="VGB76_18590"
     tRNA            8051..8137
                     /locus_tag="VGB76_18590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8084..8086,aa:Ser,seq:tga)
     gene            8153..8239
                     /locus_tag="VGB76_18595"
     tRNA            8153..8239
                     /locus_tag="VGB76_18595"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8187..8189,aa:Ser,seq:gct)
     gene            8343..8417
                     /locus_tag="VGB76_18600"
     tRNA            8343..8417
                     /locus_tag="VGB76_18600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8378..8380,aa:Arg,seq:gcg)
     gene            8428..8499
                     /locus_tag="VGB76_18605"
     tRNA            8428..8499
                     /locus_tag="VGB76_18605"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8461..8463,aa:Gln,seq:ttg)
     gene            8512..8584
                     /locus_tag="VGB76_18610"
     tRNA            8512..8584
                     /locus_tag="VGB76_18610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8545..8547,aa:Leu,seq:tag)
     gene            8597..8682
                     /locus_tag="VGB76_18615"
     tRNA            8597..8682
                     /locus_tag="VGB76_18615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8631..8633,aa:Leu,seq:gag)
     gene            8694..8773
                     /locus_tag="VGB76_18620"
     tRNA            8694..8773
                     /locus_tag="VGB76_18620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8728..8730,aa:Leu,seq:taa)
     gene            8784..8861
                     /locus_tag="VGB76_18625"
     tRNA            8784..8861
                     /locus_tag="VGB76_18625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8818..8820,aa:Leu,seq:caa)
     gene            8882..8956
                     /locus_tag="VGB76_18630"
     tRNA            8882..8956
                     /locus_tag="VGB76_18630"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8917..8919,aa:Glu,seq:ttc)
     gene            8969..9043
                     /locus_tag="VGB76_18635"
     tRNA            8969..9043
                     /locus_tag="VGB76_18635"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9001..9003,aa:His,seq:gtg)
     gene            9070..9145
                     /locus_tag="VGB76_18640"
     tRNA            9070..9145
                     /locus_tag="VGB76_18640"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9106..9108,aa:Glu,seq:ctc)
     gene            9157..9228
                     /locus_tag="VGB76_18645"
     tRNA            9157..9228
                     /locus_tag="VGB76_18645"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9189..9191,aa:Asp,seq:gtc)
     gene            9240..9312
                     /locus_tag="VGB76_18650"
     tRNA            9240..9312
                     /locus_tag="VGB76_18650"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9273..9275,aa:Lys,seq:ctt)
     gene            9362..9435
                     /locus_tag="VGB76_18655"
     tRNA            9362..9435
                     /locus_tag="VGB76_18655"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9394..9396,aa:Pro,seq:tgg)
     gene            9473..9549
                     /locus_tag="VGB76_18660"
     tRNA            9473..9549
                     /locus_tag="VGB76_18660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9508..9510,aa:Gly,seq:gcc)
     gene            9561..9632
                     /locus_tag="VGB76_18665"
     tRNA            9561..9632
                     /locus_tag="VGB76_18665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9593..9595,aa:Arg,seq:tcg)
     gene            9647..9722
                     /locus_tag="VGB76_18670"
     tRNA            9647..9722
                     /locus_tag="VGB76_18670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9681..9683,aa:Gly,seq:tcc)
     gene            9738..9810
                     /locus_tag="VGB76_18675"
     tRNA            9738..9810
                     /locus_tag="VGB76_18675"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9770..9772,aa:Cys,seq:gca)
     gene            9876..10469
                     /locus_tag="VGB76_18680"
     CDS             9876..10469
                     /locus_tag="VGB76_18680"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0100961.1"
                     /translation="MWNQMTINGRVLLLNQTYEPLGTVGVARAMVMLFNNSVTVEEWD
                     GDRVLHTPRDEYPVPSVVRRKTYINVRRRREASGMKRLRIYIRDKYRCQYCGERKLPA
                     TLTLDHIFPRSRGGDNSPLNIVTACLDCNNRKGNRTPDEARMPLLTTQSALRVKLERV
                     VLCHYAEARPEWRKYLFLDEADGNAAQTRREKNTAYI"
     gene            10536..10607
                     /locus_tag="VGB76_18685"
     tRNA            10536..10607
                     /locus_tag="VGB76_18685"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10569..10571,aa:Phe,seq:gaa)
     gene            10622..10697
                     /locus_tag="VGB76_18690"
     tRNA            10622..10697
                     /locus_tag="VGB76_18690"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10655..10657,aa:Asn,seq:gtt)
     gene            10708..10784
                     /locus_tag="VGB76_18695"
     tRNA            10708..10784
                     /locus_tag="VGB76_18695"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10742..10744,aa:Val,seq:tac)
BASE COUNT         2263 a         3294 c         3194 g         2215 t
ORIGIN      
        1 gccgctgatg accacgcccg gcgtgccgtt gccggagatg atgttgcgtt cccccggagt
       61 cgtgcccccg atgaggttgc cggtcgacct actgccgatg ctcacgccgc tcccgacagg
      121 gttcaaggcg gctgttccgg cgcggttcaa cccgatgtag ttacctttca catgattccc
      181 ggtagatgcg ccataaggcg tgtcgccgtc aatggtcaag cccccgatga tcacgttgcg
      241 cgccccgggc gtgttgcccc cgatgacgtt gttcgagccg ctaaagatgg aaaaggtttg
      301 agagccttgt ttgagggcga tgctgcccga aacgtcagtg ccgatcatgt tgcccgacac
      361 ctgattgcta tgtgagccga tgccgagatg aatatccggc ccacgattcc cggcgagcac
      421 attcccgtcg cctgccgccg cgccgccgat gagattgccg gacgatccca agagatacac
      481 gcccgcgttg ttgccgacgt gctgtgtccc gccgggatcg gttccgatgt agcagttctg
      541 gacgacattc ccgcccttct ccttcatgtg tatcccggct gacacgaaac cgttgatgac
      601 cagagatttc acgaccgtcg aaccggacag gatcgttaag ccgtgtgagt cggccttgaa
      661 taacttcgcg cccgacagga cgacgagcgg cttgccgtcg tagcccggct gggttgtgcc
      721 gtcgatgatg agaggatcgg tgatgtccgg cagcggcaac ggaaaagcga ggccaacttc
      781 tatcttctgc gctccgggcg ggagattgaa agcgatggtg tccgtgcccg gcgaagcgtt
      841 ggcggcagtg atcgcatccc tgagcgaacc ggcaccggaa tcattgttat tggtgataac
      901 aaacgtcgtg cccgtagagg cttgagtgtt gcgtgcgaag ggaaacgccg gacgtagaaa
      961 aaaaccgttc accaatacca gcagaacgag gatggacaga tatgtggctc gttttctcgg
     1021 acgcgcggag acagcctgag cacggagggg cacacgtatg aagagtcgca aagtattgtc
     1081 tccacttaga cggaagaagt ttgatttaga atctgaaata ctgctttgat gggaactgag
     1141 aggaaataca aagaccggca aaacgagact cggacgcgca ttcggaacag tgcaatttaa
     1201 gtgcttatca tccggcaggg tatttgatgg cagacagccc gattggttgc ttgaatgcct
     1261 ttgaatttca gccttaaaag caacttccgc gattcgccct gacctgtccg aatgcgtgtc
     1321 aaaaaccgct catagcttag cacgccacgc ggttaatgcc ccgcccttta acgacacgcc
     1381 gcgcaatgac aagccggaga ggggcttacc acagtccaaa ctcgcgtcac accggacgcc
     1441 ccgaaaccgt gtgtattcac ttttgaaggt tacgaaactt cgcacctcac ttcttttctc
     1501 ttcctctcta cctgtcgagc gccttcctgt ttttcgtatc tattttccaa tccgctggat
     1561 gggtgaattt tattgcgtga gggcgaaacg atttctgccg gttcgcgtat taccgtgtgc
     1621 agttcccgca aatttgttgt taccccgcaa aggagagttt taaatgaaac gcaagatcgc
     1681 agcaggcagc gtcgcgttag tggcgctcgt ggcgctgagt ctggtcgtcg catcggcggc
     1741 ggcaaaggca gacttcagcg gcaagtggtc gttggacaag acgaagagcg aaggcttgcc
     1801 gcccaacatc gaacagacca tgaccgttac gcagacgggc gacaagatcg agttagaaac
     1861 caaagttacc accccgcaag gtgagcgcgt cgtcaaggac agctacacgg tggacggcaa
     1921 agaagttgac ttcaccccgc aaggaccaca gggcgcgatt ggcaaaggca agcgcaccgc
     1981 caagtggtct gcggacggaa tgggcttgga agtgagcgag acggccacgc tcgacggccc
     2041 gaacggcgcg gacgaagttt cggccacgcg caaatggacg ctcgcccccg acagcaaaac
     2101 cctgaccatc gaactgacct tcaacggcga acagggcaca cagaaaacca agcgcgtctt
     2161 cgttaagcaa tagaaaatcc cgaccccctc taaccacacc aaaaagcccc gcctgcacat
     2221 acggcagcgc ggggcttttt ctacggaggt tagcgcgcga agaaagattt ttcagctctc
     2281 gcacaaagtt cgcacacgca cgcgctgccc gcgcagttcg ccctcccaca gcgtcttgcg
     2341 gctgcactcg tgccgcaaat atttccacag cgcgccgcgt tcgtattcgt caaggtcggc
     2401 ggggatgagg tcgaagacgg cgcgcgaggc gatgacgatc aacttccgct tcggtcgcga
     2461 gatggcgacg gtgagacggc gcggctcaag taagaagccg ctctcaagtt gtgcaaactc
     2521 gcggtcagag acagtggccg acaggatgat caattcgcgt tcgccgccct gaaaacgctc
     2581 taccgtgtca atcgagttag ccaactgcgg gagacgtgcg cgcaagagag ccttctgcgc
     2641 gcgatgcggc acgaccacgc cgatgccgcc ctcgccgccg caccctaatt tttcgcaggc
     2701 caccccggcc agttcttcca ccagagatgc ttcgaactcg ttcgactgtt gcgaaccgca
     2761 ctcgtcgtgt tcgacgacga cgagcgaatg ttcggcctcg aaggccgcgc cgagccaccc
     2821 tgcgaggcca tcgccgggca tcatgcgttc gcgcctgtcc gaacgaaaat tgatgtcgtc
     2881 ggcggcgtag atatgacgct gcaagaaggc cgcgatctct gcggggatgc gaaacgattg
     2941 gtcgagggct tcgctcttga agccgtgcgc gcgcaatgtc tcaaagatgg agaggtgcgg
     3001 gcgggcgtgt ttcaggtcgc ggcggctctc cctgtcccac gcgtgcgcga ggatgggagg
     3061 catctgccgg tggtcgccga tggcgataaa ctgcccgtcg ccgcgcagga aggccgccgc
     3121 cgtcatggcc tccgcgatgc ccatctgcga agcctcgtca acgacgacga gatcgaacaa
     3181 ctcctgcgac caatcaatgg cacgcccctt cgacgcgcct ttcttgacca gattgtaaat
     3241 cccgcccggc gtgccgccga ccacgagcag aggctcttcg agcaacatcg cccactgttc
     3301 cactgcgctc agttcgctgc cgccatcggc gaacaggcgc accactcctt ccgccaactc
     3361 ttcgcccgca tcattcgaaa tcttcgccac gcgcagacct tcaagcggcg cgagacgcgg
     3421 atcatcaggc gcggccttca acagttggcg tgcgcgtctg gcgatgctgg cgagcgcgat
     3481 tgacgtggcc gcgtgagtct tggccgcgac ggctacgcgg aacggcctcg cgggagtggc
     3541 gagcgcgagc gcacgcgcca gcaccgcgaa gccgagcgtg tggctcttgc ccgttcccgg
     3601 tggtccctgc aacacgagca cgcgctccgt caggtagtct ccgaccacgc gcgcctgcgc
     3661 ttccgtcaat ccgtacggcg cttgcgcacg cgaagcgagc gcggcgatct ccttcgcatc
     3721 gcgcagacgc ttcgggcgaa tggcgcgctg tcgcttcccc tcctccgtgt ccgcgagcca
     3781 cccgtagaga tgattcgacg cggcatggcg gcaggcatcc aaaaacttat cggcgttgag
     3841 atcgtctgcc atctcgtcaa tcgtacagac cgcgcccgcc tccggtttga acaaccagtg
     3901 cgggtagcga aactccgagt tcttgaaact catcgcgagc agccggagcc ggacgcgccc
     3961 ttcgtctaac gattcgacga cggccagccg cccgcgcacc agtttgcgtg cgggcagaaa
     4021 ctttcccgtc tcgtccgtcg caggattcag gagcacccag tcgccttcgc gaaaacggag
     4081 cgcgcacatc tctgcggcat cggtaccggt caacaattcg ccgtcgagtc tggcgaagct
     4141 gaactccgcg cgttcttccc cgcgctccga cttctcgtat gaatcgcaac ggacgacgag
     4201 cgtgcgcccg cccgccgcgc gttgttcggg cggaagcgag aggtgcagca acaactcctg
     4261 catcttcgcg tggtgttcga ggtagagaaa atcttcgagc gcacgaccga gcgtgatggc
     4321 gttcgtgtcc agttccgtct ggtcgagtcg ttcaagcgcg agcggttgtt tctcgacctg
     4381 ccggttcttg tattgaaaac tctcttcgat gaaacgaagc gcgcgacagc gttccgccgc
     4441 gagcgcgcaa aggtcttcca gactcgtgcc catgaagccg cgaatctgcg tgcgttcccg
     4501 ctcctgcatc tccggcgatt ccctgagcag cccccacgcc gcgtaagcgt attcgagcgg
     4561 aatctccgtg ccgaagcgcg cagccgattc aacccgcgcc gtgttcggcg cacgctcttc
     4621 cgccgtcgct gcgacgaact cgccgctcgc cgcgtcgcgc gcgaagagac gcttgttgtc
     4681 gaagatgcgc gcgcgaaaag ttttgagaaa ctcgcgctcc ccatcgcgcc actcgaaatt
     4741 catttcgcgc gccacttcgt agaggttctg acagaccgcg ccgaggttca gacgctcgcc
     4801 gacctcttcc gcgagaaaag agatcatccc ctgcgttagg gcgggcgtcg aagtcagcag
     4861 atcatagaag gccggaatcg cgcacagagc ctcgaagtgg cgcgcgagcg cgtccagcag
     4921 agagcgttgc ccgcgccggt cgtagagata gacatgaatg ggcgcgctcg ttgaatcggc
     4981 ggcttcggca atcgccggaa gcagacgcgc gatccagccg acgagcagcg cacgctcgct
     5041 ctccgtgtcg ggcttcgtcg gcgtgtgctc aacaatttcc gacgctcccg caggccccgc
     5101 gacgagcgcg gacaagaggt acactttatc ttccagatga tcgcgctgcg catcgacata
     5161 gactttgatt aaattcggat actgcccttc gtccggcagc gatccccaat ctgcgccgag
     5221 catgtaacgc ttcgcttcga cgtttgagcc gagacttttg agcgcggcgc gtgcgcgctg
     5281 cgccagcacg ggcagacgcg cgccgagcgg ccagcgtccg ccgatgcgcg caaagtcgct
     5341 cgccttatcc ggcgcgggca acatcgcgcc gtgcccgtag ttcatcaacg aaaaaagctc
     5401 acgcgctgtc gtcatgccca tactgatgag cgcgctcttt tccgtcgcgg taatggtcgg
     5461 gacgagagag aggtcttcgc gttcggcggt gtctatgaag cagagcgcgt tgtaggggca
     5521 gccgtcgcag tgtgcgccga gatgataaga ggcgtctgta aaacttcgcg cggcggcgcg
     5581 tgcgacatcc gaatcgggcg cggcgacgag ttgttcgatc tcgtccagat aaagcgagag
     5641 atcgaaacgt tcaattctgt cgggcgataa ttccgaaccg cgcgcggcaa tcgcgccttt
     5701 caacgtggcg ggtttgagtc cggcctctcg cagagccgct tgcaggaggc gcgcgtagaa
     5761 ggcgacctgc aaacgaaagc cgaccgtctc gcggcggctc gctttgatgt cgatgaccgt
     5821 cacatcaacg gtgtcgtcac ggtcgcccgc atgccgcacg atctctacga ggtcgggata
     5881 gccttccgag gcgcacccgc cgatgcgtcc ttcgaggcgt gcctgatagt agaagctgcg
     5941 gcttcccgcc gcctgtgatt tcaaggcggc gatgaaagcc gccgccgaag tgttctgcaa
     6001 attgtgcagc gcggcgcgcg ttgccaactc cgccacttgc tcgcgctcga acgcctgccc
     6061 cgattcggag agcaacggcg agagattttc gaagcccaca cgataacgcg ccgcgagtgt
     6121 tttagcttcc cccggaaaga gcgcgagccg caggtagcgt tcgcaacgat tacgcgaaac
     6181 gtattgcgcg agccggtgcg ctccgagcgt gcgctcggta gagatgtttt cagtggttgc
     6241 ggaatcctgc gacatgcgag ggagtttgga ggagacttct gactcggagt tgtttagtaa
     6301 acgcgtgggg cgcggctcgc cgaatcttta aacgggcaag actatttctt cgggcgcacc
     6361 accgtgagcg tcggcgtctc tgttcgctcg acggcagcga gaattgattc gcgcaggttc
     6421 gcgcccgtcg cgtcatcgtc agacagcaac ttttcgagtt gctctctgac cgggctgtag
     6481 ctcactttgc gttcaaagag cgcggcgaat ttatcaggct ccagttgctg ttcaagcgcg
     6541 ctcactttct ctggaataca tttcaagctc gccttgaaac tacacttcac cgcgccctcc
     6601 gcgccgctca aagttaccgc gcccgccacg cgctcgcgca cagaggccgc cgccttgagt
     6661 tgttccttga tctcatccag acgctccttg atccgcttct cctccccgtg caaagcaatg
     6721 tactccgaga cgagtgtttc cacttccgac tctcccggca catcacccgc tttcgctttc
     6781 aacgcctgcg cttcacaatc gaaacaatag accgcatcgt tatgcgtgcg agcttcatcc
     6841 tgcggaatca ggccgtcgca gttggcacag gcgacgtagt aatttcgggc gcacccggcg
     6901 cacaacgtct cgcctcgacg cgacgcgacg gcaacttcgg cggcgaccgc ctccagacat
     6961 tcagcgcact gaagctgtcg cagcggcgac gtgggctggc tcgcgatctg ttcgggcggc
     7021 gtgtccagcc gaatattgtc tttggcttcc aagttaattt tctccggggt taaaaaggag
     7081 gttgacgatg ttgaaccgga aacgtttgct cgccataacg ggcgcgctta aaaggctcgc
     7141 gagactgtag caaaagcctc ctccgattgt cgagagcaac gccgggagca tcctttgcct
     7201 taacaggcgc gctcgtccgc aagcgaacgc gtcgcctctt tttgttgatg gaaagagtat
     7261 ggacaaaaaa agtgttgaca atatcttctg tcgagtttat atatagctgg cgcgtacgga
     7321 agtgaaatgt gcgcgcgagg aagaacgaga aaattcaatg ttaatgaacc ggacacaatt
     7381 ccacagcatc gccgccagag gccagagggc ttcgggacgg acgcgtgccg aacagtttgg
     7441 cgaaggcttt agcgtcgcgg ctgttttgtg ccccggttcg ttaccgacct atctgcccgg
     7501 cggcgaagtc atgcgtaaag gcgcgagttc cacgattaat ctctggaatt ctgatcgtcg
     7561 gggcaatcta atttaaaaag cgggggcggc ctcccgctcc caaaatctca aaattaactt
     7621 taagagaatt gaaggagcgg gccagcttcc ctgtcgaagc taaaaggccc gctctttttg
     7681 ttgtttgata actgaattga tttttgtacg gacgagggca gcggatgtct tcagttcgga
     7741 tggtgcggcc tcggtgcttt gcgagagtcc agcaggccgc gccagccaca ggagaagtgt
     7801 gcgccgtagt gtgtctagcg gagcctcatc ttctgccctc gtgtttttta tgatagcaaa
     7861 attttcaggc gtgcgtggct gagcagcaaa agcacctgac tgtaaatcag gcgtcctgtg
     7921 gacttcgtag gtgcgagtcc taccgcacgc actcttgttt tcaaatttgg gagcatggtg
     7981 taactggcaa catgacagtc tccaaaaccg tcgctttcgg ttcgactccg gatgctcctg
     8041 tcgaagatgc ggagagtacg ccaaaacggt ctggcaccag tcttgaaaac tggcgagcat
     8101 catgctcata ggagttcgag tcttctactc tccgccaaat ctctctcgct tcggaggcgt
     8161 gccgaaactg gtagccggaa ctgtttgcta aacagtcggg cagaaacgtc ccgtgtgggt
     8221 tcaactccct ccgcctccgc taatgtaagg gatgaaggat gagggatgaa taagaaaagc
     8281 gttcaactca ttgagtgctt ctttttattc atcccttatc cctccgccct catcctttcc
     8341 ccgaacccgt agctcagcgg aatgagagcg caagcttgcg aagcttgagg tcgcgcgttc
     8401 gaatcgcgcc gggttctttc aattttttcc gggatcgtcc aaccggcagg acgcctgact
     8461 ttggatcagg agatgagggt tcgactcctt ctcccggagc ttctcaaata tgccgacgta
     8521 gcccaacgga agaggcgcta gtcttaggaa ctagtcagta agagttcgaa tctcttcgtc
     8581 ggcatcacaa ggacaagctg gagtggcgga attggcagac gcgcggcgtt gagttcgccg
     8641 cgagcaaaag tctcgttgtg ggttcgagtc ccacctccag tatcgagtta taagcggaag
     8701 tggcgtaaac ggcaaacgcg tcagtcttaa aaattgatcc ttcgggttgt gggttcgaat
     8761 cccaccttcc gcatccggca agcgcgcgtg tagcccaaac ggcagaggcg gcagtttcaa
     8821 aaactgcaca ggtgaaagtt cgaccctttc cacgcgcacc aaattttcca gatgagcctg
     8881 cggggatgta tgcaaactgg tctaagcaac ttgattttca atcaagcgtg tgcgggttcg
     8941 agtcccgcca tccctatcac gtttcgtgtc ggctgtagct caacggcaga gcgtcgcact
     9001 gtgaatgcga agacgacgag ttcgattctc gccagccgac ccaaaacttt cccctcaacc
     9061 tttaagtgcg gcgcgttagg ccagcccggt tcaagccgcc tgagtctcaa tcaggagatc
     9121 aacggttcaa atccgttacg cgctatcaat ttctatggag ctgtggccga gaggtgaggc
     9181 actccgttgt caacggagcc gatgagggtt cgagtccctc cagttccgct cacgcatacg
     9241 cgccgtaagc taaacgggtg aagcagtcgc ctcttaagcg acggagacgg ggttcgagtc
     9301 cccgacggcg caatcttttt ttccggcggg gcgcgaagcc tgaaagctcc gtcgctgcga
     9361 acggggtgtg gtgtaacgca tgcatccaag tcttgggaac ttgtggagac agttcgactc
     9421 tgtccacccc gaccactttt cacagtcagc ctgtgcgact cctcaatttt tcgcatcgtt
     9481 agtccagttg gccgaggacg cggcgttgcc aacgccgaga catgagttca aatctcatac
     9541 gatgctccag ccttttttgc gggcggttag cttaacggaa aagcggcacg cttcgaacgt
     9601 gtccgatacg ggttcgagtc ctgtaccgcc ttcttctttt cgccgcgcga gagtggtgta
     9661 acagggcaac actccggtct tccaaaccgg cattgcgggt tcaaagcccg tctctcgttc
     9721 caactatcaa cccgcacgca cccgtagcca agcggcaagg cggcagtctg caaaactgtt
     9781 aagacgcggg ttcgaatccc gccgggtgct tcgacgttac acagccgcgc atcgtctacg
     9841 tagtccgggg cgcaggccat tcaccaagaa aggaggtgtg gaatcaaatg accatcaacg
     9901 gcagagtgct cttgctcaat caaacttacg aaccgctcgg caccgtcggc gtggcacgcg
     9961 cgatggtgat gctcttcaac aacagcgtga cggttgagga atgggacggc gaccgtgtgc
    10021 tgcacacgcc gcgcgacgag tatccggtgc cgtcggtcgt gcggcgcaag acttacatca
    10081 acgtgcgccg tcgccgcgag gcatccggca tgaagcgtct gcgcatttac atacgcgaca
    10141 agtatcgctg ccagtattgc ggcgagcgca aactccccgc cacgctgacg ctcgaccaca
    10201 tcttcccgcg ttcgcgcggc ggcgacaact cgcccctcaa tatcgtgacg gcatgtctcg
    10261 attgcaacaa ccgcaagggc aaccgcacgc cggacgaggc gcgcatgccc ctcttgacga
    10321 cgcagagcgc gctgcgcgtg aagttggagc gcgtagtgct ctgccactac gccgaagcgc
    10381 gacccgaatg gcggaagtat ctctttctcg acgaagccga cggcaacgcg gcgcagacgc
    10441 gcagggagaa gaacactgcc tatatatgaa gggcgggcgt gacccgccct tcatgcctcg
    10501 cgcacgtcag gacatgaaga agacttaagc cacgcggtcg gatagctgag acggattagc
    10561 gtcgcgctga aaacgcggag aggttggttc gataccaact ccgaccatca aacgctttcc
    10621 ttgtggtgta actcaactgg tggagtgctt ccctgttaag gaagaagatg tgggttcgaa
    10681 tcccaccgcc acagccaatc tgcacctagc cggttagctc agacggccag agcgcctctc
    10741 ttacaaagag gatgtcgagg gttcgaatcc ttcaccggct accaaatttt tcggggcgtg
    10801 tctcatcccg ccagaacgcc gactgcgcgg gagcgtcgag aaccgaggtg cggctgcctc
    10861 acgccccggc caatttatcc gcaacaactt ttccgcgaca actctcgcgc cacgaatttc
    10921 gcgcgacaac tttcccggcg acgtagctcc aaccggcaga gcggct
//
LOCUS       DASWNS010000543         3773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_98413, whole genome shotgun sequence.
ACCESSION   DASWNS010000543 DASWNS010000000
VERSION     DASWNS010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98413"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..1467
                     /locus_tag="VGB76_18700"
     CDS             376..1467
                     /locus_tag="VGB76_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100962.1"
                     /translation="MAEIFHTHERLNQDTRTPHVLRTLGLSLVAHALFLAAVVYVPAV
                     RDTFNLASTLSGLKFVEEDYTKTAVGQRADVMLNFADNKLYYPAGYFSQGEFPSPDAP
                     QLIEEVRPPDPPPLPKPVRVARVKPAPTPEASPSPAEGTDEALAKNGGAPDASPTPDA
                     SPAVAAPQTPEEAERIAKEANVEKFPTINTKPLTDLLKQGKTMRDAKEIDLSGTLEME
                     VEANRQEDGRLTDVEVTGAAASNPKLLQLAKDFIAAISDSKLLAALKGTNHIKMHVKL
                     DGKEVAVRVLTEMRSESEATTMASGYNGLLFLGAMSKKGKDEEVLFKSVQIAAENKQI
                     VLTFNMPRAAAGDLLSRLIKKNETVPPPG"
     gene            1567..2265
                     /locus_tag="VGB76_18705"
     CDS             1567..2265
                     /locus_tag="VGB76_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100963.1"
                     /translation="MNGRVQRKHQRPTATAKRRQALLVRAVVGSLVLCAALAPPAPSQ
                     TALAQGVYQQDAAEVKEFRPVRALPFRQLLAKTQRMIEAGELNPQDVFDFTLEAERNA
                     DGTLRDIKFTEAAAANGRWRPLAREFITVLSDSGALSALTDADHLTFTLRLDERASAR
                     LQTVVKSNGQAAQLAQNYNALLELGRLSQRGRAGVEVLNNMVFSANGKQLTMQLEMSR
                     EMFGNLLRQQLSLP"
     gene            2495..>3773
                     /locus_tag="VGB76_18710"
     CDS             2495..>3773
                     /locus_tag="VGB76_18710"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100964.1"
                     /translation="MIDKFLTKVFGSSNQRYIKSLQPMVVRINALEPEVKKLSDEEMR
                     RRTEELKQFVAEAVADAKNPETRKQHEQDALNEILPEAFALVREASVRATGMRHFDVQ
                     LMGGIVLHQGKIAEMRTGEGKTLVATLPAYLNALTGRGVHVVTVNDYLASRDSDWMGR
                     IYRHLGLTVGCIQNDMDDWERKDAYAADITYGTNNEFGFDYLRDNMKFDLATCVQPGH
                     YYAIVDEVDSILIDEARTPLIISGASDEATDKYYKADAIIPHLKKGAETTDDSSGKKK
                     ITGDYLVDEKQHSAVLTEEGVDKAERLLGVGNLYDPSNMEMLHCVEQALKAHTLYRLD
                     HHYLVKDGEVIIVDEFTGRTMPGRRWSDGLHQAVEAKEGVKIERENQTLATITLQNYF
                     RLYEKLSGMTGTAETEAAEFDGTYKLEVAVVPTH"
BASE COUNT          806 a         1238 c         1099 g          630 t
ORIGIN      
        1 cccgttccgt ctcttcgacg ccagcccccg cgccacccgc gcgtcgcgcg cccgtttcgt
       61 cccgaccgcg ccgcctgacg tcgctgtcgc gcccgccgcc gccgacgcgt ttcccgcccg
      121 cattgttgcc gcgccgccgc ccgccgccgc gccctcctcc gtctctcgtt cgtggcatgg
      181 aggcgattgt accgcacgcc ctttgtgaaa tcgttacgag tggtgttaag tttttatgcc
      241 cccaaaaatt gcgttaagtt tttgcgcccc gaaaagttcg caacgttcgg ggcggttgag
      301 gcgtctaata catcgcaggc ttcgcgtgcg ttgacgatag aagaaccccg cgccgttcgg
      361 cctgcttcta aaattatggc ggaaattttt cacacccacg aacgactgaa tcaggacacg
      421 cgcacgccgc acgtcttgcg cacgctcggc ctgtcgctcg tcgcgcacgc gctgtttctc
      481 gccgccgtcg tgtacgtccc ggcggtgcgc gacacgttca acctggcgag cacgctctcc
      541 ggcctcaagt tcgtcgagga agactacacg aaaaccgccg tcggccagcg cgccgacgtg
      601 atgctcaact tcgccgacaa caaactctac tacccggcgg gctacttttc tcagggcgag
      661 tttccgtcgc ccgatgcgcc gcagttgatc gaagaggtca ggccgcccga cccgccgccg
      721 ctgccgaagc cggtcagggt tgcgcgcgtg aagccggccc ccacgcccga agcctcgcct
      781 tcgcccgcag agggcacgga cgaggctttg gcaaagaacg gaggcgcgcc ggatgcgtcg
      841 cccacgcctg acgcttcgcc cgccgtcgcc gcgccgcaga cgccggaaga ggccgagcgc
      901 atcgcgaagg aagccaacgt cgagaagttt cccacgatca acacgaagcc gctcaccgat
      961 ctcctgaagc agggcaagac catgcgcgac gcgaaagaga tcgatctctc cggcacactg
     1021 gagatggagg tggaggccaa ccggcaggaa gacggacgct tgacggacgt ggaggtgacg
     1081 ggcgcggcgg ccagcaaccc gaagctcttg cagttggcga aagatttcat cgccgcgatc
     1141 agcgacagca aactgctcgc cgctctgaaa ggcacgaacc atatcaagat gcacgtcaaa
     1201 ctcgacggca aagaggtcgc cgtgcgcgtc ctgaccgaga tgcggagcga atccgaagcg
     1261 acgacgatgg cgagcggtta caacgggctg ctcttcctcg gcgcgatgag caagaaaggg
     1321 aaagacgaag aggtgctctt caagagcgtc cagatcgccg ccgagaacaa gcagatcgtc
     1381 ctgaccttca acatgccgcg cgcagcggcg ggcgacctgc tctcaaggct gatcaagaag
     1441 aacgagacgg tgccgcctcc cggctaaagc gcgtcacatc cgggcgcacc cgctcgcagc
     1501 gcgtctgctg ccgccgggca acccggacga gccacacgtc gtcttcctgt gttaaaattc
     1561 cgaacgatga acggtagagt tcaacgcaag catcaacgtc ctacagcgac cgccaaacgg
     1621 cgtcaagcac ttcttgtgcg cgccgtcgtc ggctcgctcg tgctctgcgc tgcgctcgcg
     1681 ccgcccgctc cgtcacaaac agccttggcg caaggcgtct accagcagga cgccgccgaa
     1741 gttaaagagt ttcgtcctgt ccgtgcgctt ccgttccgcc aattgctggc gaagacgcag
     1801 cgcatgatcg aggcgggcga actcaatcct caagacgttt tcgattttac gctcgaagcc
     1861 gaacgcaacg ccgacggcac gctccgcgac atcaagttta cggaagccgc cgccgcgaac
     1921 gggcgttggc ggcctctggc gcgagagttc atcacggtct tgagcgacag cggcgcgctc
     1981 tccgccctga cggatgcaga ccacctgacg ttcacgctca ggttggacga acgcgcgtcg
     2041 gcgcggctgc aaacggtcgt caaatcgaac gggcaggccg cgcagttggc gcaaaactac
     2101 aacgcgctgc tcgaactcgg gcgtctcagc caacgcggtc gcgcgggcgt cgaagtcttg
     2161 aacaacatgg tcttctccgc gaacggcaag cagctcacca tgcagcttga gatgtcgcgc
     2221 gaaatgttcg gcaatctgct ccgccagcaa ctctcacttc cctgaaaaca cgcacctgag
     2281 cgcgcccgac cgcccgtgcc gcagaggcaa aagcggttcg cttgctccat tccacccgct
     2341 cacttacaat cgcatgttgc gttcaggacg caaatttgcg ctgtgcggcg cgagtcttct
     2401 gccgcgtgtt tctcaagaga gcagtgagcc atcggccacc ggctaaaacg aaaagccggg
     2461 agctgacagc taaccgctaa aaggttttaa aacaatgatt gataaatttc tgaccaaagt
     2521 tttcggcagc agcaaccagc gttacatcaa gagcctgcaa ccgatggtcg tgcgcatcaa
     2581 cgcgctggaa cccgaagtca aaaaactctc ggacgaagag atgcgccgcc gcacggaaga
     2641 actcaaacag ttcgtcgcgg aagcggtggc cgacgcgaag aacccggaga cgcgcaagca
     2701 gcacgaacag gatgccttga acgagatcct gcccgaagcc ttcgccctcg tgcgcgaagc
     2761 gagcgtgcgc gccacgggca tgcgccattt cgacgtgcag ctcatgggcg gcatcgtgct
     2821 ccatcaaggc aagatcgcgg agatgcgcac gggcgaaggc aaaacgctcg tcgccactct
     2881 gcccgcttac ctgaacgcgc tgacgggtcg cggcgtccac gtcgtcaccg tcaacgacta
     2941 tctcgcttcg cgcgactccg actggatggg tcgcatctat cgccatctcg gtctgaccgt
     3001 cggctgcatc cagaatgaca tggacgactg ggaacgcaag gacgcctacg ccgcagacat
     3061 cacttacggc acgaacaacg agttcggctt cgattacctg cgcgataaca tgaagttcga
     3121 ccttgccacc tgcgtccagc ccggccacta ttacgccatc gttgacgaag ttgactcgat
     3181 cctgattgac gaagcgcgca cgccgctcat catctccggc gcgtccgacg aagcgaccga
     3241 caagtattac aaggccgacg cgatcatccc gcacctcaaa aaaggcgcgg agacgaccga
     3301 cgatagttcg ggcaagaaga agatcacggg cgattatctc gtggacgaga agcagcattc
     3361 ggcggtcttg accgaagagg gcgtggacaa ggccgaacgc ctgctcggcg tcggcaacct
     3421 ctacgatcct tcgaacatgg agatgctcca ctgcgtcgaa caggctctca aagcgcacac
     3481 gctctaccgg ctcgatcatc actatctcgt caaagacggc gaagtgatca tcgtcgatga
     3541 gttcacgggg cgcacgatgc cgggtcgtcg ctggtcggac ggtttgcatc aggcggtcga
     3601 agcgaaagag ggtgtcaaga tcgagcgcga gaaccagacg ctcgccacga tcacgctgca
     3661 aaattatttc cgcctctacg aaaaactctc tggcatgacc ggcacggcgg agacggaggc
     3721 cgccgagttt gacggaacct acaagctcga agtcgccgtc gtccccacgc acc
//
LOCUS       DASWNS010000544         7961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_72443, whole genome shotgun sequence.
ACCESSION   DASWNS010000544 DASWNS010000000
VERSION     DASWNS010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7961
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72443"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGB76_18715"
     CDS             <1..240
                     /locus_tag="VGB76_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100965.1"
                     /translation="AWVGRAAFVSMALVAVLGWRWHNSPGGFLYALLLFVMLRVPHPQ
                     AVNELDALGRPRLLVALVTLAVFLLSFVLFPITIS"
     gene            414..1445
                     /locus_tag="VGB76_18720"
     CDS             414..1445
                     /locus_tag="VGB76_18720"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY0100966.1"
                     /translation="MIAALLLLLTAGVLYFQVWKKPLIVDSGMAKHQLTFTGHGERLF
                     EARFSPDGELLASGGVDGAVKLWRKTDGRIVRTLEHPSGVTALAFSPDGSYLATTSYD
                     SQVRLWRVADGALQKTFSGHADTVWAVAFSPDGKTVASGGEDKTIKLWDAVEGNLRQT
                     LAGHSLNIWTVAFSPDGGRLASGSFDEKINIWNVPDGKLIRTLDGHTEAVLCVEFSRD
                     GRFLVSGGDDSTVKLWQARDGSLIRSFGGDSEHIYSVAFSPDGKRILSGGRDRSTLGE
                     LWQNFFGASEADRGVTVRLWNSEDGRLLQTFAHHSNDVYSVAFSPDGGWLASASEDRT
                     VSVWQLKSD"
     gene            complement(1597..2589)
                     /locus_tag="VGB76_18725"
     CDS             complement(1597..2589)
                     /locus_tag="VGB76_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100967.1"
                     /translation="MADYRDMLDEWREAARRKARELDDKYKIKDRVEEGARVAQDAAK
                     RGATRVVSEAERARLEAERLGEEYDVRGHAGEVSEQARRAAQEAQRAAREASEKLRGA
                     GRTAGERAGEVYGEAKKRYERAARAYNVGSGVTRATSAASVGLFKAFDWARSNPKQAV
                     LVSLSIAGGLGAGAAFPRLDGVLIGAHPHWFTHSALPVWALRRAGTKFDNYLRERERL
                     VAEGELTEAERKRVEFERSIVRYVGAPLLGAFSCAAGLAICAQIFQPGNIVGAPISWI
                     LGGNPVLEGIWLFSNGIICFQQGYKFFMIALNDQQDVQRIVREIKGLLPAMATA"
     gene            complement(2709..3641)
                     /locus_tag="VGB76_18730"
     CDS             complement(2709..3641)
                     /locus_tag="VGB76_18730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0100968.1"
                     /translation="MLSKFTVLVVDDEPDKRQLLKVALEMAGYTVYTANDGAEGLAAV
                     EAQPFDLIVTDVMMPNLDGYEMVKRIRANPQTRFIPVIIQTAAKSEAQDARRGSQVGA
                     MGYITDPTDLDLLLGRAKTLLDFKNYLDSCEEAAFTDHLTGLANRRRFERQLEREVAR
                     TRRYGHPFCLLMLDIDHFKRVNDTYGHEGGDDALRRLGHVLQTGTRGVDTAARIGGEE
                     FAIILTETDFAHGLEVAERLRKTVSETDIPEAGRVTVSIGLAEFTEPVRDGRELYAAA
                     DAALYEAKRQGRDRVIVATTQAFDANPAPALGVS"
     gene            4075..4788
                     /locus_tag="VGB76_18735"
     CDS             4075..4788
                     /locus_tag="VGB76_18735"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0100969.1"
                     /translation="MPQRRACDLSLADRADIIRQSELASGLDETLVAKLAAASTAIDF
                     RRRRFVYRDGDSSDALYLIARGRVKLCRSEAETGREAVIDILPAGSLFGEYALYGSGA
                     RQMCAVAFEQARLLRIPVGDFQEAMSQSRTLYDYTFRLVGQRLARSEQRVADFALDAI
                     PARLEKLLQELSERYGQPGTDGVLIDLNLPHREIASIVGSTRESVTVRLNDMRRAGMI
                     DFVDRKILIKPPPTELATA"
     gene            4919..5119
                     /locus_tag="VGB76_18740"
     CDS             4919..5119
                     /locus_tag="VGB76_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY0100970.1"
                     /translation="MKKCPRCKKPVQWEDNPYRPFCSERCKLIDFGHWADEDYRVAAH
                     DERPPLEETEPGRDEGGGMRDE"
     gene            5259..5900
                     /locus_tag="VGB76_18745"
     CDS             5259..5900
                     /locus_tag="VGB76_18745"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0100971.1"
                     /translation="MRRLPAVVCGALALSFALAACGRTDYTASSNRAATPQTPQGERL
                     PQTSLPMPPVATAHESSSSTLAPNGVEVVHASTWTTLDGRRAKGADFQGKVLVLDFWA
                     TYCPPCRDEVPHLIALQRRYGEQGLHVVGLNVGGAEDRPKVPEFIEEFGIQYALGYPD
                     SQMSELFFADTSAIPQTYVYDRKGRLAKRFVGFDEKISAELERVVQAALEEKP"
     gene            6039..7478
                     /gene="pyk"
                     /locus_tag="VGB76_18750"
     CDS             6039..7478
                     /gene="pyk"
                     /locus_tag="VGB76_18750"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY0100972.1"
                     /translation="MRRAKILATLGPASREPATLEALLAAGANGVRINMSHGAQEEHA
                     ETIRRARAAADRMRRPLSVLVDLSGPKIRTGVLQGGQPVMLEENALFTLTTRAVAGDS
                     REVSTNYAGIARDVRPGARILLDDGAIALSVERTTETDVVCRVINGGLLNERKGINLP
                     GVVLPIPSLTDKDRRDLQFAVRAGADYIALSFVRRAEDCLEAKALIKAAGGDAPLVAK
                     IEKSEAIDHLDEIIAAADGVMVARGDLGVETSVELVPVYQKRIIEQANNAGKFVITAT
                     QMLQSMITEPRPTRAEASDVANAVWDGSDALMLSAETATGQYPVATVATMARIIESAE
                     TGRSTGSSLITKWAGKQSGRVSRAICEAAAFAAEEMNAKVIAVFTESGLMARRLSALR
                     PKQRIAALTHSREVMNELALIWAVEPHMYSPPATTEEMIHAGDRTLMEAGIVQQGQIV
                     VLMAGRLSGLGLSSSMTLHSVGEDISKQP"
     gene            7450..>7961
                     /locus_tag="VGB76_18755"
     CDS             7450..>7961
                     /locus_tag="VGB76_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100973.1"
                     /translation="MEKTSPSNLEPELISRWQISVGEVSEWLPPLLYAAAVLLSAWVL
                     SDARRRALPFYAVAAWTLATLSSPPIVFPLYLFARIFTQKGDAPTSPAGTKPEDTTTP
                     ASISDNDMTDATLAPISNDTDEQIAGRRWDSTRTLVRRKLALPLLYALALLSPGAIYF
                     YRDYHSFDAHL"
BASE COUNT         1525 a         2466 c         2355 g         1615 t
ORIGIN      
        1 gcgtgggtcg ggcgcgcggc cttcgtctcg atggcgctcg tcgcagtgct cggctggcgt
       61 tggcacaatt cgccgggcgg ctttctctac gcgctcctgc tgttcgtcat gctccgcgtc
      121 ccgcacccgc aagccgtcaa cgaactcgac gcgctcggtc gcccgcgcct gctcgtcgcg
      181 ctcgtcacgc tcgccgtctt cctgctctcc ttcgtgctgt ttcccatcac gatctcttga
      241 gtccgcttga gtcataaacg cgtccgccct gattcaattg ttaatggagc cggattgcgt
      301 gcgttagtta attgcgcgag tcgaagctgc agctcgcgca gattagtttt ggcgaaagat
      361 cgtttatgcg aagcgagacg aaaaagcgcg ggagagggaa aaaggcttta gccgtgatag
      421 cggctttgct gttgttgcta acggcgggcg ttctctattt tcaagtctgg aaaaagccgc
      481 tcatagttga tagcggcatg gcgaaacatc aactcacctt tacggggcat ggggagcgac
      541 tttttgaagc ccgtttcagc cccgacggtg aactgctcgc gagcggcggc gttgacggcg
      601 cggttaaact ctggcgaaaa acggatggcc ggatagttcg cacgctcgaa cacccgtccg
      661 gcgtcacggc tctggccttt agccctgacg ggagttatct cgccacgaca agttacgact
      721 cgcaggtcag gctgtggcgt gtggcggacg gcgcgctgca aaaaactttt tccggtcatg
      781 ccgacacggt ctgggcggtc gccttcagcc ccgacggcaa gacggttgcg agcggcggcg
      841 aagacaagac catcaagtta tgggatgccg tcgagggcaa cttacgacag acactcgcgg
      901 ggcattcgct gaacatctgg accgtcgcct tcagccctga cggcggcaga ctggcgagcg
      961 ggagttttga tgagaagatc aatatctgga atgtgccgga cggcaaattg atcagaacac
     1021 tcgatggtca tacggaggcg gtcttatgcg tcgagtttag ccgcgacggg cgtttcttag
     1081 tcagcggcgg cgatgattcg acggtcaaac tctggcaggc gcgcgacggt agtttaatac
     1141 gttctttcgg cggagactcc gaacatatct attccgtcgc tttcagtccc gacggcaaac
     1201 gaatattgag cggcggcaga gacaggagca cgctcggaga actctggcag aattttttcg
     1261 gcgcgtccga ggccgacaga ggcgtgaccg tccggctctg gaactcggag gacggacgtt
     1321 tactgcaaac gtttgcgcac cactccaacg atgtctactc ggtcgctttc agccccgacg
     1381 ggggatggtt ggcgagcgcg agcgaggaca gaacggtgag cgtctggcag ttgaaatcag
     1441 attgaagccg ggttgaacgc gtagagactc cgtcgggaaa agagaaagcc gctccaaagt
     1501 aattatggag cggcttatga ttttggaggt taaagacgag tctactttag ttacttttaa
     1561 ctttatgtcg cgcgccgcaa acgtccatgc agaccgttac gccgtcgcca ttgcgggcag
     1621 gagcccttta atctctcgca cgattcgctg cacgtcctgt tgatcgttca aagcgatcat
     1681 aaagaacttg tagccctgct ggaaacagat gatgccgttc gagaagagcc agatgccttc
     1741 gaggacgggg ttgccgccga gtatccaact gatgggcgcg ccgacgatgt tgcccggttg
     1801 aaaaatctgc gcgcagatgg caagccccgc cgcgcaactg aacgcgccga gcagcggtgc
     1861 gccgacgtaa cgcacaatcg agcgttcaaa ctccacgcgc ttgcgttcgg cctccgtcag
     1921 ctcgccctcg gccacgagtc tctcgcgctc gcgcaggtag ttgtcaaact tggtgcccgc
     1981 gcgacgaagc gcccagacgg gtagggcgga gtgtgtgaac cagtgcgggt gcgcgccgat
     2041 gagcacgccg tccagacgcg ggaaggccgc gcccgcgccc aagcctccgg cgattgagag
     2101 cgaaaccaac acggcctgtt tcggattgga gcgcgcccag tcgaaggctt tgaacagccc
     2161 cacgctcgcg gcggaagtag cacgcgtcac acctgaaccg acgttgtacg cgcgtgcggc
     2221 gcgttcgtaa cgcttcttcg cttcgccgta cacttcgccc gcgcgttcgc ctgcggtgcg
     2281 tcccgcgccg cgcaattttt cgctcgcctc gcgcgccgca cgctgcgctt cctgcgccgc
     2341 gcgccgcgcc tgttcgctca cctctccggc gtgcccgcgc acgtcatatt cttcgcccag
     2401 acgctcggcc tccagccgcg cgcgttcggc ctcgctcacc acacgcgtcg cgccgcgctt
     2461 ggccgcatcc tgcgccaccc gcgcgccctc ctccactcga tccttgatct tatacttatc
     2521 atccaactcg cgcgccttgc gccgcgccgc ctcgcgccac tcgtccaaca tatctcgata
     2581 gtcagccatg aaaacctcca ggaagaaggg atgaaggatg tgggatgagg gatgaattag
     2641 aggaagcact cactccgctt gaatgctttt tcttattcat ccctcatccc tcatccttca
     2701 tcctttgctt atgatacgcc gagcgcgggc gcggggttcg cgtcgaatgc ctgtgtggtg
     2761 gccacgataa cgcggtcgcg tccctgtcgt ttcgcttcgt agagcgcggc atcggcggcg
     2821 gcgtagagtt cgcgcccgtc gcgcaccggc tcggtaaact cggccagccc gatgctcacc
     2881 gtaacgcgcc cggcttcggg aatgtccgtc tcgctgaccg tcttgcgcag gcgttcggcc
     2941 acttcgaggc cgtgtgcgaa atccgtctcg gtcagaatta tggcgaactc ctcgccgccg
     3001 atgcgcgcgg ctgtgtcaac gccgcgcgtg ccggtctgca agacgtggcc gagccgacgc
     3061 agagcgtcgt cgccgccttc gtgcccgtaa gtatcgttga cgcgtttgaa atgatctatg
     3121 tcgagcatga gcaggcagaa ggggtgtccg taacgtcttg tgcgcgccac ttcgcgttcg
     3181 agttgccgct cgaagcgacg acggttcgcc agtcccgtca ggtgatcggt gaaggccgcc
     3241 tcttcgcagg agtcgagata gtttttgaag tcgagcaggg tcttggcgcg accgagcagg
     3301 agatcgagat cggtcgggtc tgtgatgtag cccatcgcgc cgacctgtga accgcgccgc
     3361 gcgtcctgcg cttcgctctt ggccgccgtc tggatgatga cggggatgaa gcgcgtctgc
     3421 gggttggcgc ggatgcgctt gaccatttcg tagccgtcga ggttgggcat catcacgtcg
     3481 gtcacgatca gatcgaacgg ctgcgcttcc accgccgcca gcccttccgc gccgtcgttt
     3541 gccgtataga cggtgtaacc cgccatctcc aaagcgactt tgagcaactg ccgcttatcc
     3601 ggctcgtcgt ctactaccag cacagtgaat ttgcttaaca tagttcccgc tcacgtctgc
     3661 ccggacagag ttgaaaagta atatcccgtc ggttgggtcg ccgaagatta tcgcacgctg
     3721 ccggtgccgc gtgacgaatc cggcaaccca ttttccggtc atcactactt gataaagagg
     3781 tcttgcatgc gcccgttctt catgttagag tgcgttttac caaggacggt tgccgttgtt
     3841 gcccacgttc aatctcgtta cttacctgaa tcctttcgca gatgcacgaa taaatcttaa
     3901 caacgactaa aagtgctcgc gtgcgtgttt acaccagcag cagacctacg gcttccttgc
     3961 ccatttgtta atcaactttt tctcagcttt caaggcgcct ctccttgcat gtcccgcttt
     4021 agaagctgaa cttgattttc cttaacaaac actactttcg ggagtctccc tattatgccg
     4081 caacgaagag cctgcgatct gagtcttgca gaccgcgcag acattatacg ccaatccgaa
     4141 ctcgcctccg gtctggatga gacgctcgtg gcgaaactgg ccgccgcctc caccgccata
     4201 gactttcgcc gccgccgttt cgtctatcgc gacggcgact cgtccgatgc gctttatttg
     4261 atcgcgcgcg ggcgcgtcaa actgtgccgc agcgaagcgg agacgggtcg ggaagccgtc
     4321 atcgatattc tgccagcggg ttcgctcttc ggcgaatacg cgctctacgg gtcaggggca
     4381 agacagatgt gcgccgtcgc ctttgagcag gcgcggctgc tgcggattcc ggtgggggat
     4441 tttcaggagg ccatgtctca aagccgcacg ctttatgatt acactttccg tctggtcggt
     4501 caacgactag cgcgttccga gcagcgcgtg gccgattttg cgctcgatgc cattccggcg
     4561 cggctcgaaa aactcctgca agaactctcc gaacgttacg gccagcccgg caccgacggc
     4621 gtgctcatcg atctcaatct gccccaccgc gaaatcgctt ccatcgtcgg ctcgacgcgc
     4681 gagagcgtca ccgtccgctt gaatgacatg cgccgcgcgg gtatgatcga tttcgttgac
     4741 cgcaaaattt taatcaagcc gccgccgacc gaactggcga cggcgtgagc aaggaaaagc
     4801 cgggcgaggg gaaatgggta aaggttaaag gaaaggcaaa tgcacgggat tccagacgct
     4861 gcttttgcct ttctcctttt tcctcgaccc gtctttctct ccccgccagc catgtcttat
     4921 gaagaagtgc ccgagatgca aaaagcccgt ccaatgggaa gataacccgt accgcccctt
     4981 ctgctccgaa cgctgcaaac tgatagactt cggccactgg gctgacgaag actatcgcgt
     5041 cgccgcacac gacgaacgcc ccccgctcga agagacggag ccaggaaggg atgagggcgg
     5101 agggatgagg gatgaataag aaaagcactc aagtgagtgg aatgctttct ctggttcatc
     5161 cctcatcctt catccttttc atggtcgtcg tgtagaatac ggctcgcctc ccacaacaca
     5221 ggagttctgg agttagtatg tcactgcgtt cttatccgat gagacgtttg cccgcagtcg
     5281 tttgcggcgc acttgcgctg tcgttcgcgc tcgccgcgtg cggcagaacc gattacacgg
     5341 cttcttcaaa cagagccgcg acgccacaga cgccgcaagg agaacgtctg ccgcaaacct
     5401 cgctcccgat gccgcccgtg gcgacggctc acgaatcatc ttcctctacg ctcgcgccga
     5461 acggcgtcga ggtggtgcac gcttcgacgt ggacgacgct cgacgggcga cgcgcgaagg
     5521 gcgcagactt ccaagggaag gtgctcgtgc tcgatttctg ggcgacctat tgcccgccgt
     5581 gccgcgacga agtgccgcat ctcatcgctt tgcagcgtcg ctacggcgag cagggcttgc
     5641 acgtcgtcgg cctgaacgta ggcggcgcgg aagaccgtcc gaaagtgccg gaattcatcg
     5701 aagagttcgg cattcaatat gccctcggct acccggactc tcaaatgtcc gaactatttt
     5761 tcgcagacac ctccgcgatc ccgcaaacct acgtctatga tcgcaagggg cgtctcgcca
     5821 aacgcttcgt cggcttcgac gaaaaaatct ccgccgaact cgaacgcgtc gtgcaggcag
     5881 ccctggagga aaaaccttga cgagtgacga gtgacgagtg acaagttaaa acaaaagcct
     5941 tgtctttaaa cttatctttt ttcttgctcg tcattcatca ctcgtcactg tattcaaaac
     6001 ttgtcacctg ttacccgtca ctggtcactg tatttaaaat gagaagagcc aaaatcctcg
     6061 ccacgctagg gcctgcatcg cgcgaaccgg cgacgctcga agcgttgctc gccgcgggcg
     6121 caaatggcgt tcgcatcaac atgtcgcacg gcgcgcagga agagcacgcc gagacgatcc
     6181 gccgcgcacg cgccgccgcc gaccgcatgc gccgcccact ctccgtcctc gtcgatctct
     6241 ccggtcccaa gatcaggacg ggcgtgttgc agggcggtca gcccgtcatg ctcgaagaga
     6301 acgcgctgtt tacgctgacg acgcgagccg tcgcgggcga ctcgcgtgaa gtctcgacca
     6361 actacgcggg catcgcgcgc gacgtgcgac ccggcgcgcg catcctgctg gacgacggcg
     6421 ccatcgcctt gagcgtcgag cgcacgacgg agacggatgt tgtctgtcgc gtcatcaacg
     6481 gcggtctttt gaacgagcgc aagggcatca atttgccggg ggtcgtcctg ccgattccct
     6541 ctctcaccga taaagaccgg cgcgacttgc agtttgccgt ccgcgcgggc gcagactata
     6601 tcgcgctctc cttcgtccgt cgcgccgaag attgcctcga agcgaaagcg ttgatcaaag
     6661 cggcgggggg cgatgcccca ctcgtcgcca agatcgaaaa gtcggaggcg atagatcatc
     6721 tggacgagat catcgccgcc gccgacgggg tgatggtggc gcgcggcgac ctcggcgtgg
     6781 agacgagcgt ggaactcgtc cccgtctatc aaaaacggat catcgagcag gcgaataacg
     6841 cgggcaagtt cgtcatcacg gcgacgcaga tgttgcaatc catgatcacc gagccgcgcc
     6901 ccacgcgcgc agaggcttcg gacgtggcga acgccgtgtg ggacggttcg gacgcgttga
     6961 tgctgtcggc tgagacggcg accggacaat atccggtggc gactgtggcg acgatggcgc
     7021 gcatcatcga atcggcggag acagggcgca gcaccggatc gagtttgatc acgaaatggg
     7081 cgggcaaaca gtcgggtcgt gtgagccgcg cgatctgcga ggcggcggcc ttcgcggcgg
     7141 aagagatgaa cgcgaaagtg atcgcggtct tcacggagtc ggggctgatg gcgcgtcgtc
     7201 tctcggctct gcgtcccaag cagcgcatcg ccgcgctcac acattcgcgc gaggtgatga
     7261 acgaactcgc gctcatctgg gcggttgagc cgcacatgta ctcgccgccc gccacgaccg
     7321 aagagatgat tcatgcgggc gaccgcacgc tcatggaagc gggcatcgtg cagcagggac
     7381 agatcgtcgt gctgatggcg ggcagacttt cagggttggg gctatcgagt tcgatgacgc
     7441 tgcactcggt tggagaagac atctccaagc aaccttgagc cggaattgat ctcgcgctgg
     7501 caaatctccg tcggggaagt atccgagtgg ctgccgcctc tgctctacgc ggcagcggtg
     7561 ctcctgtcgg cgtgggtgct ctcggacgcg cgccgtcgcg ccctgccctt ctacgccgtc
     7621 gctgcatgga cgctcgccac gctcagctcc ccgcccatcg tcttccccct ctacctcttc
     7681 gcgcgcatct tcacccaaaa gggcgacgcg ccaacttctc ccgcaggcac aaagcccgaa
     7741 gacaccacga cacccgcgtc aatctcggac aacgacatga ccgacgcaac gctcgcacca
     7801 atctcaaacg acacggacga acagatcgcc ggacgacggt gggacagcac acgcacgctc
     7861 gtccggcgca aactcgcgct gcccctgctc tacgcgcttg cgctgctctc ccccggcgcg
     7921 atctattttt accgcgacta tcacagtttc gacgcgcatc t
//
LOCUS       DASWNS010000545         6775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_489437, whole genome shotgun sequence.
ACCESSION   DASWNS010000545 DASWNS010000000
VERSION     DASWNS010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489437"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="VGB76_18760"
     CDS             <1..1587
                     /locus_tag="VGB76_18760"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0100974.1"
                     /translation="EEREVFTAPEFVQSMIGRGILGNKTKGGFYRKQKGEGEKQEIWT
                     LDHASLEYRPQQKVKLPALELAKNIEDVGERIKSLVWSKDRVGAFLWKTVSRTLRYAA
                     NRIPEIANNVVDVDRAMRWGFNWELGVFETWDAIGVEKSVARMREEGASVPSNVERML
                     EAGAKSFYKFEDGQRFYFDFDKLAYQPVNEPAGVLVLKSVKDRTGVVKKNAGASLIDI
                     GDGVACLEFHSKMNAIGGDTILMIKQSLAEVEKNFVGLVVGNQAQYFSVGANLMLILL
                     EAQEENWEDLDLMVREFQRGTMSLRYSSKPVVTAPFGLVPGGGCEVAIHGDRVRASAE
                     VYIGLVEAGVGVIPAGGGTKEMLLRALDSIPKGMDDADPFPFVKRAFETIALAKVATS
                     ADEARQLGYLREEDSVSMNQDRLIADAKQEVLALARGGYTEPQPRTDILALGNSALAT
                     LKLGIHQMKRGGFISEYDAHIGEQLARILTGGDLNHPTRVSEQYLLDLEREAFLSLCG
                     QRKTQERMAAMLKTGKPLRN"
     gene            1658..2143
                     /gene="ybaK"
                     /locus_tag="VGB76_18765"
     CDS             1658..2143
                     /gene="ybaK"
                     /locus_tag="VGB76_18765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEY0100975.1"
                     /translation="MDYPVTPAVRLLREKKVEFEPHLYHYEERGGTRHSAESLGVDEH
                     AVVKTLVMETETRKPLVVLMHGDREVSTKNLARTIGTKSVQPCDPQTAQKHTGYLVGG
                     TSPLGTRARLPVYVERTIFSLPKIYINGGKRGFLVSLSPQDLRVLLTVEEVEVAIESH
                     G"
     gene            2127..2600
                     /locus_tag="VGB76_18770"
     CDS             2127..2600
                     /locus_tag="VGB76_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100976.1"
                     /translation="MKVTAREAFACVCSLSTPAQAFARADVVFSGRISEAQKGKWKIA
                     LERVWKGEVEAEIRLRELSVGSSCRSDFTLGKRYIVFASIDATRRKTTYSPDACSWTI
                     PFKTYGQQDADKTVRLMKDFDPTKTVYWMEDWLIQNLKELGAEKAPIKKRGERKR"
     gene            2597..3778
                     /locus_tag="VGB76_18775"
     CDS             2597..3778
                     /locus_tag="VGB76_18775"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0100977.1"
                     /translation="MRDAVIVSAVRTAVGKAPKGTLRDTRPDEMGATVISEALRRAEG
                     VELNEVEDVIMGCAMPEAEQGMNVARAAAIRAGLPVETSAMTINRFCSSGLQSIAIAA
                     DRIRTGAAEVIVAGGLETMSLIPMGGHTIRPNPYLVEHYPDFYLNMGLATENVARKYE
                     VSREEQDEFSYGSHRRAAAAQDAGKFADEIVPLKIVFEELDERGRKQRREVVFEKDEG
                     VRRDTSAESLSKLRPAFHAKGTITAGNASQMSDGAAAAVVMSDERARALGLRPMARFV
                     AYATAGCPPEEMGIGPVYAIPKVLKIAGLTQEQIDVIELNEAFAAQSLAVIRTLGLDP
                     EKVNVNGGAIALGHPLGCTGAKLTATALRELERRNGRYAMVTMCVGGGMGAAGIFERL
                     S"
     gene            3794..5572
                     /locus_tag="VGB76_18780"
     CDS             3794..5572
                     /locus_tag="VGB76_18780"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0100978.1"
                     /translation="MSAVAEEKKIIVGGSFLIEERTPEEVFTPEDFTEEHRMIAETTR
                     EFMDNEVRPATDELEKHNWQLARELIRKSADLGMVSVNIPEEYGGLGLDQTSGAIVGE
                     LLGRSASFATTLGAQSGIGLTPILYFGTEAAKKKYLPKIASGELVSAYALTEAGSGSD
                     AMAAKATARLSEDGTHYILNGEKMWITNGGFADVFIVFAKIDGDKFSGFIVERQEGLT
                     NGADEHKMGIKGSSTTALILNDAKTPVENLLGEAGKGAKIAFNILNIGRFKLGAMCNG
                     GMKLMIHEAVRYANERQQFGRPISSFGAIKAKLAEMAIKTWVGEAMVYRTLGLIENAT
                     SGETDAQAKLRGIEEYAAECSIIKVALSEYCDFVADEMVQIYGGYGYSADYPAERAYR
                     DSRINRIFEGTNEINRMLIPGRMMKSALSGRLALLPAAQKLMDEVLSPSLPSFDEDET
                     VLATELKLVQNAKKVALMTLGTAAQKYMAKLSDEQEILMGVADIVMDVYAMESAILRA
                     QKLASNKGEEAAARYLDMTRVFCSDAVSRIEARAKTTLAAMSEGDELRTLLAALRRFT
                     KNTPINTVAARQRIADVLIKANKYAY"
     gene            5688..6656
                     /locus_tag="VGB76_18785"
     CDS             5688..6656
                     /locus_tag="VGB76_18785"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0100979.1"
                     /translation="MRIIPISLPTPFYIGPVNVYLIAEEPVTLIDTGPKTREALEALR
                     EGLRRARFRVHDVRRIVLTHAHEDHCGLAKSLRDEAKDAEVLVHRWETGHRKGRLEYE
                     EHRALLVRAGVPSDEVEAMRRMYEGVRQFADALEDEEHRELTDESELEFESGSLRVVH
                     TPGHTPGSCSFLREADRTIIAGDCVLKRVTPNPVLSPDPVDPSRRFPSLAEYLVSLAR
                     LRAFAPTLVHGGHGEAVEDFEELFTRYLRAIRERQAEVIRLVPKAGASAWEVAREMFP
                     GTEDVHRFLAVSEAVAHLDLAHAENRLALELSADGREVYRNRLAGD"
BASE COUNT         1422 a         1840 c         2271 g         1242 t
ORIGIN      
        1 gaggagcgcg aggtcttcac cgcgccggaa tttgtgcaga gcatgatcgg gcgcggcatc
       61 ctcggcaaca agacgaaagg cggcttctat cgcaagcaga agggcgaagg cgagaaacag
      121 gagatctgga cgctcgacca cgcctcgctc gaataccgtc cgcagcagaa ggtgaaactg
      181 cccgcgctgg agttggcgaa gaacatcgaa gacgtgggcg agcggatcaa gtctctggtg
      241 tggtcgaagg atcgcgtggg cgcgtttctg tggaagaccg tctcgcgcac gctgcgttac
      301 gccgcgaatc gcattccgga gatcgcgaac aacgtggttg acgtagaccg cgcgatgcgt
      361 tggggcttca actgggaact cggcgtcttt gagacgtggg acgcaatcgg cgtggagaag
      421 tccgtcgcgc ggatgcgtga ggaaggcgcg agcgtgccct cgaacgtcga gcggatgctg
      481 gaagccggag cgaagagttt ttataagttt gaggacggcc agcgctttta tttcgatttc
      541 gacaaactcg cttaccagcc ggtcaacgaa ccggcgggcg tactcgtgtt gaagtcggtc
      601 aaggatcgca cgggcgtggt taaaaagaac gcgggcgcgt cgctcataga catcggcgac
      661 ggcgtggcgt gtctcgaatt tcattcgaag atgaacgcca tcggcggcga caccatcttg
      721 atgatcaagc agtcgctcgc cgaagtggaa aagaatttcg tcggcctcgt ggtcggcaat
      781 caggcgcagt acttttccgt cggcgcaaac ctgatgctga tcttgctgga agcgcaggag
      841 gagaactggg aagacttaga cctcatggtg cgcgagtttc agcgtgggac gatgagcctg
      901 cgttattcgt cgaagccggt ggtgaccgcg ccgttcggac tcgttcccgg cggcggttgc
      961 gaagtggcga ttcacggcga ccgcgtgcgc gcttcagccg aagtttatat cggtctggtc
     1021 gaagcgggcg tcggcgtgat tccggcgggc ggcgggacca aagagatgct cctgcgcgcg
     1081 ctcgattcga tccctaaggg catggacgac gccgacccgt tccctttcgt caaacgcgcg
     1141 ttcgagacga ttgcgctggc gaaggtggcg acctctgcgg acgaagcgcg ccaactcgga
     1201 tacctgcgcg aagaggattc cgtctcgatg aatcaggatc ggctgatcgc agacgcgaag
     1261 caggaagtgc tcgcgttggc tcgtggaggc tacaccgaac cgcagccgcg cacagacatc
     1321 ctcgcgctcg gaaattccgc gctcgccact ctgaaactcg gcatccatca aatgaaacgc
     1381 ggcggcttca tctccgaata cgacgcacac atcggcgaac aactcgcgcg catcttaacg
     1441 ggcggcgatc tcaaccaccc gacgcgcgtg agcgaacaat atctcctcga tctcgaacgc
     1501 gaggctttcc tctcgctctg cggccagcgc aagacgcagg aacgcatggc cgccatgctc
     1561 aagaccggaa agccgctacg gaactgatct caagtcttaa gtcgcaagtc tgaaaacaat
     1621 ctgttgactt gcgacttatg actttcgact tgcgactatg gattatccgg taacgcccgc
     1681 agtcaggttg ctgcgcgaga agaaggtgga atttgagccg cacctctatc attacgagga
     1741 gcgcggcggc acgcgacact ccgccgaatc tttaggcgtt gacgagcacg cggtcgtgaa
     1801 gacgctcgtg atggagacgg agacgcgcaa acccctggtt gtgttgatgc atggcgaccg
     1861 tgaagtctcc acgaagaatc tggcgcgcac gattggcacg aagagcgtgc agccctgcga
     1921 cccgcagacg gcgcagaaac acacgggcta tctggtcggc gggacaagcc ctttggggac
     1981 gcgtgcgcgc ctgccggtct atgtcgagcg gacgatcttc tcgttgccga agatttacat
     2041 caacggcggc aagcgtggct ttttagtttc gctcagcccg caggacttgc gtgtgctgtt
     2101 gacggtcgaa gaagttgaag tcgccattga aagtcacggc tagagaagcc tttgcctgcg
     2161 tgtgctcttt atccacccct gcacaggcat tcgccagagc cgatgtcgta ttttcgggaa
     2221 ggatttcgga agcgcagaag ggcaaatgga aaattgcgtt ggagcgagtt tggaagggcg
     2281 aggtggaggc agagatcagg ttgcgtgaac tttccgtcgg gagcagttgt aggagtgatt
     2341 tcacgttggg aaagcgatac atagttttcg ccagcattga tgcaacccga cggaaaacaa
     2401 catacagccc ggacgcttgc agttggacaa ttccctttaa gacttacggc caacaggacg
     2461 cggataagac ggttcgtttg atgaaagact ttgacccgac gaagactgtc tattggatgg
     2521 aagattggct catacaaaat ctgaaagaac tcggagcgga aaaagctccc attaaaaaga
     2581 ggggcgaaag aaagagatga gagacgcagt tatcgtatca gcggtgcgca cggcggtcgg
     2641 caaagcgccg aagggaacct tgcgcgacac gcgacccgat gagatgggcg caaccgtgat
     2701 cagcgaagca ctcaggcgcg ccgaaggcgt ggagttgaat gaggtcgaag acgtgatcat
     2761 gggctgcgcg atgccggagg cggaacaggg catgaacgtg gcgcgtgcgg cggcgattcg
     2821 cgcggggctg cccgtggaaa cttcggcgat gacgatcaat cgcttctgct cgtcggggct
     2881 gcaatcaatc gcgattgcgg cggatcgcat caggacgggc gcggcggaag tgatcgtggc
     2941 gggcgggttg gagacgatgt ctctgattcc gatgggcggg cacacgattc gcccgaaccc
     3001 gtatctggtc gagcactacc cggattttta tttgaacatg ggactcgcca ccgagaacgt
     3061 cgcgcgcaag tatgaagtgt cgcgcgagga gcaggacgag ttctcctacg gctcgcaccg
     3121 gcgcgcggcg gcggcgcagg acgcaggcaa gtttgcggac gagatcgtgc cgctcaagat
     3181 cgtcttcgaa gagttggacg agcgcgggcg caagcagcgg cgcgaggtcg tatttgagaa
     3241 agatgaaggc gtgcggcgcg acacttcggc ggagagcctg tcgaagttgc gcccggcctt
     3301 tcatgcgaag ggcacgatca cagcgggcaa tgcctcgcag atgtcggacg gggcggcggc
     3361 agcggtcgtg atgtccgatg aacgcgcgcg tgcgctggga ctcaggccga tggcgcgttt
     3421 cgtcgcgtat gccacggcgg gctgcccgcc cgaagagatg ggcatcggtc ccgtgtatgc
     3481 gatcccgaag gtcttgaaga tcgcggggct gacgcaagag cagatcgatg tcatcgaact
     3541 caacgaagcc ttcgccgcgc aatcgctcgc agtgattcgc acgctcggac tcgaccccga
     3601 aaaagtgaac gtcaacggcg gcgcgattgc gctcggccac ccgctcggct gcacgggcgc
     3661 gaagttgacg gcgaccgccc tgcgcgaact cgaacggcgc aacgggcgtt acgcgatggt
     3721 gacgatgtgc gtcggcggcg gcatgggcgc agccggaatt tttgaacgac tcagttaaga
     3781 aaggatcaaa cttatgtcag ccgtagccga agagaaaaag ataatcgtgg gcgggagttt
     3841 tctgatcgag gagcgcacgc cggaggaggt gttcacgcca gaggatttca cggaagagca
     3901 ccgcatgatc gcggagacga cgcgcgagtt tatggacaac gaagtgcgcc ccgcgaccga
     3961 cgaactcgaa aagcacaact ggcaactcgc gcgtgagttg atccgcaagt cggctgatct
     4021 ggggatggtc agcgtcaaca tcccggaaga gtacggcggc ctcggactcg atcagacgag
     4081 cggcgcaatc gtcggcgaac tgctgggtcg ttccgcttcg tttgccacca cgctcggcgc
     4141 gcagtcgggc atcggtctta cgccgatcct ctatttcggc acggaagcgg cgaagaaaaa
     4201 gtatctgccg aagatcgctt cgggcgagtt ggtgtcggcc tacgcgctga ccgaggccgg
     4261 gtctggttcg gatgcgatgg cggcgaaagc cacggcgcgt ctcagcgaag acggcacgca
     4321 ctacatcctg aacggcgaga agatgtggat cacgaacggc gggttcgcgg acgtgttcat
     4381 cgtcttcgcc aaaattgacg gcgacaagtt cagcggcttc atcgtcgagc gtcaggaagg
     4441 gctgacgaac ggcgcggacg agcacaagat gggcatcaaa ggttcatcca cgaccgcgct
     4501 catcctgaac gacgcgaaga ctcccgtgga gaatctgctg ggcgaagcgg gcaagggcgc
     4561 gaagatcgct ttcaacatcc tgaacatcgg tcgcttcaaa ctcggcgcga tgtgcaacgg
     4621 cgggatgaag ttgatgattc acgaagcggt gcgttacgcg aacgagcggc aacagttcgg
     4681 gcgtccgatc tcttcgttcg gcgcgatcaa ggcgaagttg gcggagatgg cgatcaagac
     4741 gtgggtgggc gaggcgatgg tctatcgcac gctcggcctc atcgagaacg ccacgagcgg
     4801 cgagacggac gcgcaggcga aactgcgcgg catcgaagag tatgcggcgg agtgttcgat
     4861 catcaaggtg gcgctgtcgg agtattgcga tttcgtcgcc gacgagatgg tgcagattta
     4921 cggcggctac ggttattcgg ctgactatcc ggcggagcgc gcctatcgcg attcgcgcat
     4981 caaccgcatc ttcgaaggca cgaacgagat caaccgcatg ttgattcccg gtcgcatgat
     5041 gaagagcgcg ctctcaggcc ggctcgcgct cttgcccgcc gcgcagaagt tgatggacga
     5101 agtgttgtcg ccctcgcttc cgtcgttcga cgaagacgag actgtgctcg ccacagagtt
     5161 gaaactcgtg cagaacgcga agaaggtggc cttgatgacg ctcggcacgg cggcgcagaa
     5221 gtacatggcg aagctctcgg acgagcagga gattttgatg ggcgtcgcgg acatcgtgat
     5281 ggatgtctat gcgatggagt cggccatcct gcgcgcgcag aaactcgcgt cgaataaggg
     5341 cgaagaggcg gctgctcgct atctcgacat gacgcgcgtt ttttgcagcg acgccgtctc
     5401 tcgcatcgaa gcgcgcgcca agacgacgct ggccgccatg agcgaaggcg acgaactccg
     5461 cacgctcctc gcagccctgc gccgcttcac gaagaacacg ccgatcaaca ccgtcgccgc
     5521 gcgccagcgc atcgccgacg tgttgatcaa ggcgaacaag tacgcctatt agtcaaaagg
     5581 tgaaaggccg gaggggaagg ggtaagtgag agagacaagt gttcgaactt gcacgtcgct
     5641 ttccctttcc cctttaacct ttccccctga ccctttatat tcctgccatg cgcatcatcc
     5701 ccatctcctt gccgacgccg ttctatatcg gccccgttaa cgtctatttg atcgcggaag
     5761 agcctgtgac tttgatcgac acgggaccga agacgcgtga ggcgttggag gctctgcgcg
     5821 agggtttgag gcgcgcgcgt tttcgcgtcc atgatgtgcg gcgcatcgtc ctgacgcatg
     5881 cgcacgaaga ccattgcggg ttggcgaagt ctctgcgcga cgaggcgaag gatgcggagg
     5941 tgctcgtgca caggtgggag acgggacacc ggaaagggag gctcgaatac gaagagcatc
     6001 gcgcgttgct cgtgcgcgcg ggcgtgccgt cggatgaagt cgaggcgatg cggcggatgt
     6061 atgagggggt gcggcaattt gcggacgcgc tcgaagatga ggagcacagg gaactgacgg
     6121 acgagtcgga gttggagttt gagagcggga gtttgcgcgt ggtgcacacg ccgggacaca
     6181 cgccggggtc ctgctctttc ctgcgcgagg ccgaccgcac catcatcgcg ggcgactgcg
     6241 tgttgaagcg cgtgacgccg aaccccgtgc tctcgcccga cccggttgat ccttcgcgcc
     6301 gttttccttc gctcgccgaa taccttgtga gcctcgcgcg cttgcgcgcg ttcgcgccga
     6361 cgctcgtgca cggcgggcat ggcgaagcgg tcgaggactt cgaggaactg ttcacgcgct
     6421 acctgcgcgc gatccgcgaa cgacaggcgg aggtgattcg tctcgtgccg aaggcgggcg
     6481 cgagcgcgtg ggaagtggcg cgcgagatgt ttccggggac ggaggatgtg catcgcttcc
     6541 tcgccgtctc ggaggcggtg gcgcatctcg atctggcgca cgcggaaaac agactcgcgt
     6601 tggagttgag cgcggacggg cgggaggttt atcgcaacag gttggcgggc gactgagtgc
     6661 gcgtcgtgcg catggggcgt atgcacgcat gcgaagcgcg tttgagtctc gatattaatc
     6721 tgacaattaa aacagccgcc gcgcttcatg ttgaatgaag cgcggcggct gttta
//
LOCUS       DASWNS010000546         8788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_254634, whole genome shotgun sequence.
ACCESSION   DASWNS010000546 DASWNS010000000
VERSION     DASWNS010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254634"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1026
                     /locus_tag="VGB76_18790"
     CDS             184..1026
                     /locus_tag="VGB76_18790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0100980.1"
                     /translation="MRTIVHLSDLHFGRIDQAIIEPLAATVTEIKPDVVVVSGDLTQR
                     ARSEQFQEAREFLDELPHPQIIVPGNHDVPLYNIFQRFLEPLDKYRRYITNDLEPFYA
                     DEQIALLGINTARSLTIKDGRVNEGQIARIRERLCPFGDEITKFIVTHHPFDVPEGHE
                     READLVGRAQMAMEALATCGADVLLSGHLHVSHTGHTAKRYKISGYSALVVQAGTATS
                     TRVRGEVNSFNIIRVEHPYIEVKRLAWQPDRAAFSTASIENFRHTPEGWVRLADGAES
                     GIRE"
     gene            1257..2495
                     /locus_tag="VGB76_18795"
     CDS             1257..2495
                     /locus_tag="VGB76_18795"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100981.1"
                     /translation="MFDIKRRSRRQSFLLTFIIFVALIPGAPAANLFAQGVVNYEAEK
                     QRALKLLENSKATEALPILERLTQSNPEDGQAMFYFGFAILAQSQSLTELNTRKQSRI
                     RARAAMLKARELGYKSPLLDSILESIPADGGDEQKYSANAEADRAMREGEAAFVKGNL
                     DAALASYQRALELDPKIYEAALFAGDMYFKKGQPFKAAEWYERAIKIAPDRETAYRYS
                     ASPLMEAGKLEEARARYIEAVVAEPFSRLAWAGLARWAGAARVELAHPKIEVPTNLTT
                     LKDNQPTMSIDAGVLAGKEDGTIAWLGYGLTRVGWAKHKFAKEFPGEKEYRHTLREEA
                     EALRAVIESVKAQTKDNKIKTLDPSLARLVKLHDEGLLEAYILFARVDAGIARDYFEY
                     RQTNRDKLRRYLSEYVTAKK"
     gene            complement(2634..4367)
                     /locus_tag="VGB76_18800"
     CDS             complement(2634..4367)
                     /locus_tag="VGB76_18800"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0100982.1"
                     /translation="MFRRKIAVALLLACSVLSNSFPVMAQQPSATPAAATQTPAPDPN
                     DPIARIKDEGMNRSQVMQTLSYMSDVIGPRLTNSPGMKRANEWTRDQMTKWGMQNAHL
                     EPWGPFGRGWTLKRFSAEVVEPLNVPLIAYPKAWSPGTNGALNAEVVYVDATDDAGLE
                     KYRGKLKGAIVLTAPIREIKAHFEALGTRMTEKELLALADAPDPATAVRRPGQQPTPE
                     QIAQFIFSLKKANFLMEEGAALLVDPGRGDGGTIFVQSANVPQPVPQVADLTSLQAFF
                     RRGVSAYDKSAPKLVPQIVVAGEHYNRMMRMLQAGEKVRMNVNLDVQFQDQDLMGYNT
                     IAEIPGGDLKDEVVMLGGHLDSWHSGTGATDNAAGCAVVMEAARILQTLGLKPRRTIR
                     VALWSGEEQGLLGSRAYVKEHFGEIAQPSPQPTPAAGAAGQPAARPGGAPQGRLVTKP
                     AYEKFAGYFNLDNGTGKIRGVYLQGNEAVRPLFRSWLMPFRDLGAQTLSISNTGGTDH
                     LAFDAIGLPGFQFIQDEVEYETRTHHSNQDVFDRIQADDMKQAATIMAAFVYQAAMRD
                     EKLPRKPAPPR"
     gene            complement(4571..5632)
                     /locus_tag="VGB76_18805"
     CDS             complement(4571..5632)
                     /locus_tag="VGB76_18805"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY0100983.1"
                     /translation="MATSRMADVLVLGGGPGGSATAIACARAGLRVALVEREPFPREH
                     VGETLHPGVEPLLEQLGVADEVRRAGFLRHEGNWIEWAGERRFEAFGADAKTGAWQGW
                     QAWRADFDAILLARARALGVRIFQPCQAVRPLLDDARVAGVETSHNSITAVFVIDAAG
                     DRHWLARHLGLEIKKYSARLTARYGYAEGACASRDDAPQIVSDTRGWTWTARVRPRLY
                     QWTRLSVAVEEGGEGATGGVRVEACVPAEFRGLKRRGAAKGADVTWRRAARPAGAGYF
                     LVGDAAAVLDPASSHGVLKAIMSGIMSAHLIAHVFARRVSELEAAQRYCDWLAEWFEH
                     DVARLKELYAIFDAPARTL"
     gene            complement(5619..7928)
                     /locus_tag="VGB76_18810"
     CDS             complement(5619..7928)
                     /locus_tag="VGB76_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100984.1"
                     /translation="MALIDQVAAACKRLASHGWRELLLAHGLDITAADLAAELSRTLP
                     DIRRSVKGFEDFALEGRKGVTPGQPAQSLLYHAFASSNVVMRADGSRLTLFPTLAEIE
                     AVENYVFSVDAPSLQQLKNRAGAGARLAVVVFAYEYRPASQTCHKKHADLVFARTGVA
                     RVGTSPAFYDAERRGFIPADEKNQFAMRVSPARYAAFIAVAAKGSRSNFCPLRFREAG
                     AGRSQSDANRSFWQPLHKLFSGAECLRGLPQGKLDLSLTGSHLNEKLRRIHLTLSAQH
                     GSPGVPFDTGWKEPDISQSPFRFTRGIAEFSTKAEYGQGVLVPVAHQKLVEPASYKGK
                     PLSFNVPPGVGTLSSSVEIPWDAGALRAPEYIHARHRVRDNGTIENLNDIDKVRARVQ
                     AGGYKALHYLDFTGDGAIEARCPQLVGQAAISAFIPAYSLVTAPDFFPTCDQRELTEW
                     TDTIEPGFLKDVWETTPDTLSDQRLAANLQLPNSPFKPADNTVTAIVPMFGKHAAQQT
                     VPKTADTLRHSHMPDNSAGVFAPGWDVSRDRAPDGTWHLSAYGLGSPFPEDAKLCAAF
                     STFWPAAAPDATRTFTFGPLSDRYFTVSPLTDEEIGQVGNLPWDGAPGPKVVTSGGSE
                     FAEYASFDHVDYVENALQNKFSLRVTAHVDLEEYQRRVLAMALAYRILGGTKIDWIVL
                     SFRRVNPGTPELIEAQAEAQLTLPDRVYRFEVFTNPKQETIPGNQFRKRRLAIKGRQT
                     LFVDADNRRVLSKAATAGAKWKGREIGNL"
     gene            complement(8219..>8788)
                     /locus_tag="VGB76_18815"
     CDS             complement(8219..>8788)
                     /locus_tag="VGB76_18815"
                     /inference="COORDINATES: protein motif:HMM:NF013555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEY0100985.1"
                     /translation="GEEAETCELCAKPMALKRGRFGQFLGCTGYPDCRNIRKIDRKGV
                     VAAAPVPLDETCPVDGVHLVRRQGRFGEFVSCSNYPTCKFIKRETTGIACPRPGCKGE
                     IVVKKSKRGKVFYGCSEYPKCDAVYWDKPVSEPCPQCHAPFLLEKTTKKGTTRSCANE
                     ECDYKTEPLPEVPPATKPDVSGRGSRVAT"
BASE COUNT         1663 a         2763 c         2761 g         1601 t
ORIGIN      
        1 gcacgcgcct gcgcgtcgcc acggacggcg aagtcaccat catgaagacg ccgctccatt
       61 accgagtccg ccccgccgcg ctgcgggtca tagctccaaa aagcagtgga cagtgaatgg
      121 taattggtga aagataaaac cggcttttac tattcaccac ttaccattta ccattcacca
      181 atcatgagaa ccatcgtaca tctctccgat ctgcattttg gacgaatcga tcaggctatc
      241 atcgagccgc tcgcggcgac cgtgacggag atcaagccgg acgtggtggt cgtctccggc
      301 gacctgacgc agcgtgcgcg ctcggaacag tttcaggagg cgcgcgagtt tctggacgag
      361 ttgccgcacc cgcagattat cgtgcccggc aatcacgacg tgccgctcta caacatcttc
      421 cagcgtttcc tcgaaccgct cgacaagtat cgacgctaca tcacgaacga cctcgaaccg
      481 ttttacgccg atgagcagat cgcactgctc ggcatcaaca cggcgcgctc gctgacgatc
      541 aaggacgggc gcgtcaacga agggcagatc gcgcgcatcc gcgaacgtct ctgcccgttc
      601 ggcgatgaga tcacgaagtt catcgtcacg catcacccgt tcgacgtgcc ggaaggtcac
      661 gaacgcgagg cagacctcgt ggggcgcgcg cagatggcga tggaggctct ggcgacgtgc
      721 ggcgcggacg tgctgctctc cggccacctg cacgtcagcc acacggggca cacggcgaag
      781 cgttacaaga tcagcggcta ctccgcgctc gtcgtgcagg cgggcacggc aacctccacg
      841 cgcgtgcgcg gcgaagtcaa ctccttcaac atcatccgcg tcgagcatcc gtacatcgaa
      901 gtcaaacgcc tcgcatggca acccgaccgc gccgccttct cgacggcctc catcgaaaac
      961 ttcagacaca cgcccgaagg ttgggtgcgg ctcgcagacg gggcggagtc cggcatcagg
     1021 gaatagaggg aagggtgaaa gaagaaaggt aaaacggcgt cgcttcgtgc cggtgcgcgg
     1081 cgacgcccct cttgagtctc agccaacact tgctcaacct ctttcctttc tccccttcac
     1141 ccctttccct ttacccttct taattgacac tcgcgcggcc tgtccctaga ctcccgctag
     1201 tacgcctgac tgagactttt ccggattcgt ccgcatctcg caaaaaggag tcgtgcatgt
     1261 ttgacatcaa acgccgctcg cgtcgccagt cctttctcct caccttcatc atcttcgtcg
     1321 cgctgattcc gggcgcgccc gccgccaatc tcttcgcgca gggcgtcgtc aactacgaag
     1381 ccgaaaagca gcgcgcgctc aaacttctcg aaaactccaa ggcgaccgaa gccctcccga
     1441 ttctcgaacg gctcacgcag agcaacccgg aagacgggca ggcgatgttc tatttcggct
     1501 tcgccatcct cgcgcagtcg cagtcgctca cagaactgaa cacgcgcaaa cagtcgcgca
     1561 tacgcgcgcg cgccgcgatg ctcaaggcgc gtgagttggg ttacaagagt ccgctgctcg
     1621 attcgatcct tgagtcgatt ccggcggacg gcggagacga gcagaaatat tcggccaacg
     1681 cggaggcaga tcgtgcgatg cgtgaaggcg aagcggcctt cgtcaaaggc aacctcgacg
     1741 ccgcgctcgc ctcttaccag cgcgccctcg aactcgatcc gaaaatctac gaagccgcgc
     1801 tcttcgccgg cgacatgtac ttcaaaaagg ggcagccctt caaagccgca gagtggtacg
     1861 agcgcgccat caagatcgcc cccgaccgcg agacggctta tcgctattcg gccagccctc
     1921 tgatggaggc gggcaaactc gaagaggcgc gggcgcgcta catcgaagcg gtcgtggcgg
     1981 agcctttcag ccgtctcgcg tgggcggggc ttgcgcgctg ggctggggcg gcgcgcgtcg
     2041 aactcgcgca cccgaagatc gaagtgccga cgaacctgac gacgctcaaa gacaatcagc
     2101 cgacgatgag cattgatgcg ggtgtgctgg caggcaaaga agacggcacg atagcgtggc
     2161 tcggctatgg gctaacgcga gtgggttggg caaagcataa gtttgccaaa gaatttccgg
     2221 gcgagaagga gtatcgccac acgctgcgcg aagaggcgga ggcgttgcgc gcggtgatcg
     2281 aatcggtgaa agcgcagacg aaggacaaca agatcaagac gctcgacccg tcgctcgcgc
     2341 gcctcgtcaa actccacgac gagggcttgc tcgaagccta catcctcttc gcacgcgttg
     2401 acgcaggcat cgcgcgcgac tacttcgaat accgccagac caaccgcgac aaactgcgcc
     2461 gctacctgag cgagtacgtc acggcgaaga agtaagaacg cgaagaagta agaagtggta
     2521 aaggggaagg gcggaaagcc acgagaggaa gcggggcgca gcgtttgaat gctgcgcccc
     2581 gctttttgtc tttaaccttt tccctctaac ccttctcctt taccctgtcg ttcttagcgc
     2641 ggcggggcgg gtttgcgcgg gagtttctcg tcgcgcatgg cggcttgata gacgaaggcg
     2701 gccatgatgg tcgcggcctg tttcatgtcg tcggcctgta tgcggtcgaa gacatcctga
     2761 ttcgagtggt gcgtgcgcgt ctcgtactcc acttcgtcct gtatgaactg gaagccgggg
     2821 aggccgatgg cgtcgaaggc gaggtggtct gtgccgcccg tgttggagat ggagagcgtc
     2881 tgcgcgccca ggtcgcggaa gggcatgagc cacgaacgga agagcgggcg gacagcctcg
     2941 ttgccctgca aatagacgcc gcgaattttg cccgtcccgt tgtcgaggtt gaagtagccc
     3001 gcaaacttct cgtacgcggg cttcgtcacc agcctgccct gcggcgcacc gccggggcgc
     3061 gcggcgggtt ggcctgccgc tccggcggcg ggcgtgggtt gcggcgaagg ctgcgcgatc
     3121 tcgccgaagt gctctttgac gtaagcgcgc gagccgagca agccctgctc ttcgccgctc
     3181 cagagggcga cgcgaatggt gcgtcgcggt ttgagtccga gcgtctggag gatgcgcgcg
     3241 gcttccatca cgacggcgca accggcggcg ttatccgtcg cccccgtgcc gctgtgccac
     3301 gaatccaggt ggccgccgag catcaccact tcgtctttca ggtcgccgcc cggaatctct
     3361 gcgatggtgt tgtagcccat caagtcctga tcctgaaact gcacgtcgag gttgacgttc
     3421 atacgaactt tttcgcccgc ttgcagcata cgcatcatgc ggttgtagtg ctcgcccgcg
     3481 acgacgatct gcggcaccag tttcggcgcg ctcttgtcat aagccgaaac gccgcgacga
     3541 aagaacgcct gcaaggaggt caggtcggcc acctgaggga cgggctgagg cacgttggcc
     3601 gactgcacga agatcgtgcc gccgtcgccg cgacccggat cgacgagcaa ggccgcgccc
     3661 tcttccatca aaaagttggc cttcttcaag ctgaagataa actgcgcgat ctgttcgggc
     3721 gtcggctgtt gaccgggacg ccggacggca gttgccggat cgggcgcgtc ggccagcgcg
     3781 agcagttctt tctccgtcat gcgcgtgccg agcgcttcga agtgcgcttt gatctcgcgg
     3841 atgggagcgg tgagcacgat ggcgcccttg agtttgccgc gatacttctc caatccggcg
     3901 tcgtccgtcg cgtccacgta gacgacctcc gcgttgagcg cgccgttcgt gcccggcgac
     3961 caggctttcg ggtaggcgat gagcgggacg ttcagaggct ctacgacttc cgccgagaaa
     4021 cgcttcaaag tccagccgcg cccgaaaggt ccccacggtt cgaggtgcgc attctgcatg
     4081 ccccacttcg tcatctggtc gcgcgtccat tcgttggcgc gcttcatgcc gggcgagttg
     4141 gtgaggcgcg gcccgatcac gtcggacatg taacttaaag tctgcatcac ctgcgagcga
     4201 ttcatgccct cgtccttgat gcgcgcgatg gggtcgttcg gatcgggcgc gggagtctgg
     4261 gttgcggctg cgggtgtggc ggacggttgc tgcgccatga ccgggaagct gttcgacaga
     4321 accgaacagg ccagcagcaa ggcgacggcg atcttgcgtc gaaacataaa ataggctcct
     4381 aaattattga ctgaaaattt aaacctgcga acaggcgagc ggcaataccc ttcaagggaa
     4441 cttgattata ctctaacgcg ggcgggaaag tttcaacgcg cgggcgtttc ccgttgcaga
     4501 ttgaaaggag gacgagtcag gccgacgcgt cctccgaatt ttagagacag tgcgggaatt
     4561 cccgtcagga ctaaagcgta cgggcgggcg cgtcgaagat cgcatagagt tccttcaggc
     4621 gcgcgacatc gtgctcgaac cattccgcga gccagtcgca atatctctgt gcggcttcaa
     4681 gttccgacac gcggcgcgcg aagacgtgcg cgatgagatg cgccgacatg atgcccgaca
     4741 tgatggcttt caaaacgccg tgcgacgagg ccggatcgag gacggcggcg gcatcgccca
     4801 cgagaaaata gcccgcgccc gccggacgcg ccgccctgcg ccacgtaaca tccgcgccct
     4861 tcgccgcccc gcgccgcttc aacccgcgaa actccgcagg cacgcacgct tcgacacgca
     4921 cgccccccgt cgcaccttcg ccgccttctt ctacggccac agacaaccgc gtccattgat
     4981 acagacgcgg gcggacgcgc gccgtccagg tccaaccgcg cgtatccgag acgatctgcg
     5041 gagcgtcgtc gcgcgaggcg cacgcgcctt ccgcgtaccc gtagcgcgcc gtcagccgcg
     5101 ccgaatattt tttgatctcc agtccgagat ggcgcgcgag ccagtggcgg tcgcccgccg
     5161 cgtcgataac gaaaacggct gtgatggagt tatgtgaagt ttccacgccc gcgactctcg
     5221 cgtcgtcgag cagcgggcgc acggcctggc agggttggaa gatacgcacg ccgagggcgc
     5281 gtgcgcgtgc gagcaggatc gcgtcgaagt ctgcgcgcca cgcctgccag ccctgccatg
     5341 cgcccgtctt cgcgtccgcg ccgaaggctt caaagcgacg ctcgcccgcc cactcgatcc
     5401 agttgccctc atggcgcaaa aaccctgcgc ggcgcacctc gtccgccacg ccgagctgtt
     5461 cgagcagcgg ttcgacgccg ggatgcagcg tctctccgac gtgctcgcgc gggaacggtt
     5521 cgcgctcgac cagtgcaact cttagtcccg cgcgtgcgca ggcaatggcc gtcgccgaac
     5581 cgcccggccc gccgccgagc accaacacgt cggccatcct agaggttgcc aatctctctg
     5641 cccttccact tcgcgcccgc cgtcgcagcc ttcgagagca cgcgccggtt atccgcatcc
     5701 acgaagagcg tctggcgtcc cttgatcgcc agacgccgtt tgcgaaactg gttgccgggg
     5761 atggtttcct gcttcgggtt ggtgaagacc tcgaaacgat agaccctgtc gggtaaggtc
     5821 aactgcgcct ccgcctgcgc ctcgatgagt tcgggcgtgc cggggttgac gcgccggaac
     5881 gagagcacga tccagtcgat cttcgttccg cccagaatcc ggtaggccag cgccatcgca
     5941 agcacgcgcc gctggtactc ttcgaggtca acgtgcgccg tcacgcgaag cgagaactta
     6001 ttctgcaaag cgttctcgac ataatcgacg tgatcgaagc tggcatactc tgcgaattcg
     6061 ctgccgccgc tcgtgacgac ctttggaccc ggcgcgccgt cccacggaag attgccgacc
     6121 tgcccgatct cttcgtcggt cagaggactc accgtaaaat agcggtcgct caagggtcca
     6181 aaagtgaaag tgcgcgtcgc atcgggggcg gcggcaggcc agaaggtgct gaacgcggcg
     6241 cacagtttcg cgtcctctgg aaaagggcta cccaatccat aagccgagag gtgccaggtg
     6301 ccgtcgggcg cgcggtcgcg gcttacgtcc cagccgggcg cgaagactcc ggcagagtta
     6361 tcaggcatgt gtgaatggcg cagcgtgtct gccgtcttcg gcacggtctg ctgcgcggcg
     6421 tgcttgccga acatggggac aatggccgtg accgtgttgt ccgctggctt gaaaggactg
     6481 ttcggcagtt gcaggttcgc ggcgaggcgt tgatcggaga gcgtgtcggg cgtggtttcc
     6541 cagacatctt tgagaaaacc cggctctatc gtgtcagtcc attcggtgag ttcgcgttga
     6601 tcgcaggtgg ggaagaagtc gggcgcggtg acgagcgagt aggcgggaat gaaagcggag
     6661 atggcggcct gtcctaccag ttgcgggcag cgcgcttcta tcgcgccgtc gcccgtgaag
     6721 tcaagatagt gcagagcctt gtagcctccc gcctgaaccc gcgcccggac tttatctatg
     6781 tcgttgaggt tttcgatggt gccgttgtcg cgcacgcgat ggcgcgcgtg gatgtattcg
     6841 ggcgcacgca gcgcgcccgc gtcccaagga atctcgacgc ttgaagagag cgtgcccacg
     6901 cccggcggca cgttgaaaga gagcggcttg cccttgtagc tcgcaggctc gaccagtttt
     6961 tgatgtgcga ccggaaccag cacgccctga ccatactcgg ccttagtgga aaactccgcg
     7021 atgccgcgcg taaagcgaaa cggcgactgg ctgatgtctg gctccttcca acccgtgtcg
     7081 aagggcacgc ccggcgagcc gtgttgcgcg ctcaacgtga gatgaatgcg gcgcaatttc
     7141 tcgttgagat ggctgccggt gagcgagaga tcgagtttgc cctgcggtaa gccgcgcagg
     7201 cactccgcgc cggagaagag tttatgcaga ggttgccaga aagagcggtt cgcgtcgctc
     7261 tgcgacctgc ccgcgcccgc ctcgcgaaaa cggagcgggc aaaagtttga gcggctgccc
     7321 ttcgccgcca ccgcgatgaa ggcggcgtag cgcgcgggcg agacgcgcat ggcgaattga
     7381 tttttctcgt ccgccggtat aaagccgcgc cgctctgcgt cgtagaaagc gggagaagtg
     7441 ccgacgcgtg cgacgcccgt gcgcgcaaag acgaggtctg cgtgtttctt gtgacaggtc
     7501 tgcgaggccg ggcgatattc gtaggcgaag acgacgacgg cgagacgcgc gcctgcgccc
     7561 gcgcggttct tgagttgttg cagcgaaggc gcatcaaccg agaagacgta attttcgacg
     7621 gcttcaatct cggcgagcgt cgggaagagc gtgagccgcg aaccgtccgc gcgcatgacg
     7681 acgttcgaag aggcgaacgc gtgatagaga aggctctgcg cgggttgtcc cggcgtgacg
     7741 cccttgcgtc cttcgagcgc gaaatcttca aaacctttca cgctgcgcct gatgtccggc
     7801 aaggtgcggc tcaactcggc ggccagatcg gcggcggtga tgtcgagtcc gtgcgcgagc
     7861 agcagttcgc gccagccgtg cgaggcgagg cgtttgcagg ccgccgcgac ttgatcgatg
     7921 agagccataa gttgtgatcc tttctcgtga gacttgccgc aggagttgag gaaacgctta
     7981 acaggaagaa ctttcggggg gcgactaaag agttttacga ggatgaacga accggcgcaa
     8041 ctttcaagaa cgaagcgggc gcagtttaaa cagcgctgcg cccgcccgtc aaccaaattt
     8101 ttcgctcccc gatgcacccg acacaacacc tcgacggggc gcgtgcgagc gcaaaaaaga
     8161 gaggaggacg aaccgcgcgg ctcgtcctcc tccatcgggt ttcctgctca ctgctaactt
     8221 aagtggcgac gcgcgaaccg cgcccgctca cgtccggctt cgtcgcgggc ggcacttcgg
     8281 gcagcggctc tgtcttgtag tcgcactctt cgttggcgca cgagcgcgtc gtgcccttct
     8341 tcgtcgtctt ctccaacagg aacggcgcgt ggcattgcgg gcaaggctcg ctgacgggct
     8401 tgtcccagta aaccgcgtcg cacttcggat actccgagca gccgtagaag actttcccgc
     8461 gcttcgactt cttcaccacg atctcgccct tgcagccggg gcgcgggcag gcgatcccgg
     8521 tcgtctcgcg cttgatgaac ttgcacgtcg ggtagttgga gcaggacaca aactcgccga
     8581 agcgtccctg tcgcctgaca aggtgcacgc cgtccacagg gcacgtctca tcgagcggca
     8641 cgggcgcggc ggccacgacg cccttacgat caatcttgcg tatgttgcgg caatcgggat
     8701 aacccgtgca gccgaggaac tggccgaagc gtccgcgctt caaagccatc ggcttcgcgc
     8761 acaactcgca cgtctccgcc tcttcgcc
//
LOCUS       DASWNS010000547        11989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_125051, whole genome shotgun sequence.
ACCESSION   DASWNS010000547 DASWNS010000000
VERSION     DASWNS010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11989
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125051"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1701
                     /locus_tag="VGB76_18820"
     CDS             283..1701
                     /locus_tag="VGB76_18820"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0100986.1"
                     /translation="MSRNQKNYLPVDLAGENQAQRSAGSSRNSKDEIGNVISLQTRMN
                     AQRATQASAQVAASQDFEADLVCLSHLRWDFVYQRPQHLLSRCAKARRVFFVEEPLFI
                     QGPSRLDVSQREDGVYVVVPHLPEGLSSEVSVEAIQQAMIDRLFAEYHIRDYVLWYYT
                     PMALGWTRHLKPLVTVYDCMDELAAFKNAPRLLREREADLFRRADLVFTGGQSLYEAK
                     RNQHQNVYAFPSSIDRAHFAQARDSRMEDPADQANIPHPRMGFFGVVDERFDIELLDG
                     ISEARPDWQFVIIGPVVKIDENALPRRANIHYLGSKSYKELPAYLAGWDVAALLFARN
                     ESTRFISPTKTPEYLAAGKPVVSTSIRDVVRPYGKLGLVRIADDAASFIRAAAEIGMD
                     ERTDDTTWLARVDEFLAKISWDRTWGEMAELIGEVAASRRATIAASAQQSKPATVTTT
                     VKQAGVAARAATASSSSVAAAD"
     gene            1712..2845
                     /gene="glf"
                     /locus_tag="VGB76_18825"
     CDS             1712..2845
                     /gene="glf"
                     /locus_tag="VGB76_18825"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEY0100987.1"
                     /translation="MRETGMFDYLIVGAGYAGSVLAERLARVSGKRVLVCDRRPHIAG
                     NAYDHYNDAGVLVHKYGPHIFHTNSREVFEYLSQFTEWRQYEHRVLASVDGQLLPIPI
                     NLDTINQYYGLNLTSFQVEEFLNSVAEKREQIRTSEDVVVNRVGRELYEKFFRNYTRK
                     QWGLDPSELDASVTARVPIRTNRDDRYFADSYQAMPLHGYTRMFEKMLDHPNIKIMLN
                     TDYRDIERIIPYKEMIYTGPVDEYFDFRHGKLPYRSLEFKHETKDTEVYQSAPVVNYP
                     NDNLYTRITEFKYLTGQEHRKTSIVYEYPRAEGDPYYPVPRPENAELYAKYKTLADET
                     PGVHFVGRLATYKYYNMDQVVAQALTTYKKITGERDKANVPKS"
     gene            2872..5136
                     /locus_tag="VGB76_18830"
     CDS             2872..5136
                     /locus_tag="VGB76_18830"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY0100988.1"
                     /translation="MSRGASLPAPELELWAGVECTVNRVGDQFYDQLERNGHARRIED
                     LDLFATLGVRAMRYPVLWERTAPNGLARADWSWPDARLARLRELGIRPIVGLLHHGSG
                     PRQTSLVEPDFPTQLAAYARGVAERYPWVDAYTPVNEPLTTARFSGLYGHWYPHGHDD
                     KTFVRALVGQCRATALAMRAIREINPHAQLVQTEDLGKTFSTRTLRYQADFENVRRWM
                     SLDLLCGRITREHQMWDYLTRIAGIGERELEWFNAHPCPPDVLGINHYLTSERFIDER
                     VERYPDRAVGGNGRHRYVDVEAVRVCAEGTAGARVLLREAWERYGIPLAVTEAHLGCT
                     REEQLRWLGEVWDAARSLRADGVDLRAVTAWTLLGAFDWCSLLTRCTGKYEPGVFDVR
                     SGTPRPTALARMLRDLAEKGEHDHPALDSPGWWRRLERLQYPPVRRQPATKVSTNKQG
                     LDMRRGDARPLIINGATGTLGRAFARLCQQRGLAYRLLTRAEMDIADPQSVELALAEF
                     EPWAVVNTAGYVRVDDAEREPEKCFRENGEGAATLAAACARRGAALLTFSSDLVFDGT
                     KGSPYVESDEVSPLNVYGRSKAEAERRVLAESPSALVLRTSAFFGPWDEHNFITVALR
                     TLAEGRKFVAADDAFVSPTYVPDLVGASLDLLIDGERGIWHLANEGAITWAELARRAA
                     QLAGLDPQLVEARSTVFLQLSAPRPLYSVLGSERGQLLPTLEDALERYLNERETRFAS
                     VSTASAGQKQGRAG"
     gene            5388..6170
                     /locus_tag="VGB76_18835"
     CDS             5388..6170
                     /locus_tag="VGB76_18835"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0100989.1"
                     /translation="MATSETALTQGQIDRGEAPQGYARDVNVGEVERWASAIGGGALA
                     IYGLTRGTYGGIALALLGASLVYRGTTGHCNAYDALGINTAASERDNPFVSVEGGRGV
                     KVEKSVTINKSPEELYNFWLNFENLPRFMDHLEEVRLTGDGRSHWVAKGPAGTSVEWD
                     AESYNLKENELIAWRSLEGSQVANAGSVHFTPAPGGQGTEVRVVLKYDPPAGVLGAWV
                     AKLFGEAPEQQIEEDLRRFKGLMETGETATTAGQSSGRSAKA"
     gene            6237..6398
                     /locus_tag="VGB76_18840"
     CDS             6237..6398
                     /locus_tag="VGB76_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100990.1"
                     /translation="MADENRSESGAGGTQGNASSGGAEEQSGDPGRTPGTAEGDEETV
                     DESLKQKES"
     gene            6702..8885
                     /locus_tag="VGB76_18845"
     CDS             6702..8885
                     /locus_tag="VGB76_18845"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY0100991.1"
                     /translation="MIEFTPRRLHPAPWLQLACACLLIFAAGASYSAFAAPVILTEAT
                     TTRAIALDAVTKFRDPFPLTTPGYTAGADTRTRIMFFVLNLDLLAGEGANALTADAED
                     SAGRKYVFVVENVSSVPGFEALKQVTVRLSNDVGDVGDLLVRINLHGVASNRVRISVG
                     HAGGGIPDDPGSVPAPAPAAPPAPTPTPTPNPYTAAATNADTVRLLEQATWGATNAEV
                     ARVQSMGFRAYLNEQFNAPMSSYPNLPLYPTDQAQGCPTGSAVTCGRDNYSMYPLQVK
                     FFQNALATGAGSDQLRQRVAFALHQVFVVSGRDINYPAWMAYYLQILDRNAFGNFRNV
                     LQEITLNPSMGEYLNMRGNLRTNPNENYAREILQLFSIGVDELNLDGTPKLDAEGRRI
                     PSYSQDTINNFARVFTGWNLAANKQAVINGVAINVFNYQDPMIVSRETNHDTASKQLL
                     NGQILPPNQTSAVDLNAALDNIFNHPNTAPFIGKQLIQHLVTSNPSPAYVERVARVFN
                     NNCDALYPESCTNTRGDLKAVVRAVLLDPEARGDVKTDPKYGKLREPVQLITNVLRAF
                     NATSDGVLANRTTASGGDLPNQLDQPLFLPATVFSYYSPTYEVPGVNLLGPSFEILST
                     STSLRRANVINTLIYSGVPASPPNVPTGTQINLAAFEALAANPAQLISALDALLMHNT
                     LSAAARASITTAINSIPTTDANFARKRVQAAAYLVATSHQYQVQR"
     gene            8951..10372
                     /locus_tag="VGB76_18850"
     CDS             8951..10372
                     /locus_tag="VGB76_18850"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0100992.1"
                     /translation="MASTRRDFLRQTGCALVGGAAFASGIEQLGLINALAQGSSTATD
                     YKALVCIFLSGGNDGNNTVVPVDAARYAEYANVRSAAGLAIPAPGQTGGLLPINPLSG
                     GAYGLHPSMPELKTLFDNRQLAVVSNVGPLVEPLTRDTYRSGSGKRPLQLFSHSDQVA
                     QWQTSVSHDASQTGWGGRTADRTATLNGAATFPQVVSIAGTTIFATGANTRPLGISDS
                     RTALTSVLPLNNPPGAAGYTAAQRDARRAAFDQMRNLDLGATLVKASRDTTEQAIQTS
                     IALATANATLATVFPDTTLGYQLEQVARLVKLRDTLSIKRQIFFCALGGFDTHSGQTA
                     ANGQGALLTQVSQAMKAFYDATVELGIQNNVTAFTLSDFGRTLQPAGTGAGAVGSDHG
                     WGNHHFIMGGAVRGGDFYGRFPTLALGGPDDTDTRGRWIPSISVEQYAATLASWYGLA
                     ASDLPLVFPLIGRFSTANLGFMS"
     gene            10395..>11989
                     /locus_tag="VGB76_18855"
     CDS             10395..>11989
                     /locus_tag="VGB76_18855"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HEY0100993.1"
                     /translation="MRKIYQLKLCITALFFVAGAGVLLTNNFTTPSVHAFSAGPPAGY
                     TRAPGEEPESCRECHLPQEAGTGNISVNAPQTYVPGQTYQISVVHTNNDATRRRWGFQ
                     LTALDDTNQKAGTLAPLADGLTQVLNNQGPFPNRQYIEHTSNGNFDGQTGGATWTFNW
                     TAPPADVGPVIFYAAGNHANGNGNSDGDHIYFTFSTIAPAAASGDFSLNITPATQTIL
                     PGASGTYTVTVTPSNNFTGTVNLSVLGLPSGASATFDPASVQITDANARSSTLTINNG
                     STTLLGNFTLTVNGASGSLQHAASAFLVTGPTMFDASLNVRILARNLNQPIALASIGA
                     DDLFVLEKASGRVLRVTNGAAQSAVLDLAVNSASERGLLGIALHPNFPANPRVYLYWT
                     ESSTGADTTNPDETPLLGNRVDSFLWNGATLTPDRNLIKLRALQQDAGQPSRGNHNGG
                     VIRFGLDGKLYIIMGDNGRRGLMQNLPFGPAVNAQGPLTADDQFGGPEPDNAHLSGVV
                     LRLNDDGTVPADNPFFNAATNLTG"
BASE COUNT         2431 a         3894 c         3532 g         2132 t
ORIGIN      
        1 taagcgttaa attttcggat tgcagaatct cacagagagg ttcatttccc ccgtaattac
       61 aggttcttcg tgcattcgtt gacctgccac caacccccgg cgcgcaaggg cacattactt
      121 gctcttaagc tgcgaagttc agtagcaact gctcaggcta cgtttccact tcaaggtgtt
      181 cgattactcc aacggagtcg gacatgatca agctaaggtc gaagacaaaa gggtcttaat
      241 tcgtcgtgcg ccatttttag gagagaggtt acgagagaaa ctatgagtcg caatcaaaaa
      301 aattatcttc cggtcgatct agcgggggag aatcaggcgc agagaagcgc cggcagcagc
      361 aggaatagta aagatgagat cggcaacgtc atctctttac agacgcgtat gaacgcgcag
      421 cgcgcaacgc aagccagcgc gcaggtcgcg gcgtcgcagg attttgaagc tgacctggta
      481 tgcctgtcgc acctgcgctg ggacttcgtc tatcaaagac cgcagcactt gttgagccgt
      541 tgcgcgaagg cgcgcagggt cttcttcgtc gaagaaccgc tcttcattca gggtccttca
      601 cgtctcgacg tgagccagcg cgaagatggc gtgtatgtcg tcgtgccgca cctgcccgaa
      661 ggtctgtcga gcgaagtctc ggtcgaagcg attcagcaag cgatgattga tcgcctgttt
      721 gcggagtatc acatacgcga ttacgtgctc tggtattaca cgccgatggc tctcggctgg
      781 acgcgccatc tgaaaccgct cgtgaccgtc tatgattgca tggacgaact cgcggccttc
      841 aagaatgcgc cgcgcctctt gcgcgaacgc gaagccgacc tgtttcgtcg cgccgacctc
      901 gtcttcacgg gcgggcagag tctctacgaa gccaagcgca atcaacacca gaatgtctac
      961 gcgtttccgt cgagcatcga ccgcgcgcat ttcgcgcagg cgcgcgacag ccgcatggaa
     1021 gaccctgcgg atcaggcgaa catcccgcac ccgcggatgg gcttcttcgg cgtggtggac
     1081 gagcgtttcg acatcgaact tctcgacggt atctcggaag cgcgccccga ttggcagttc
     1141 gtcatcattg gtccggtggt gaagattgac gagaacgctt tgccgcgtcg cgccaatatt
     1201 cattatctcg gcagcaagtc ttacaaagaa ctgcccgcgt acctggctgg ctgggatgtg
     1261 gccgcgctgc tgtttgcgcg caacgaatcg acgcgcttca tcagcccgac gaagactccg
     1321 gaatatctgg cggcgggtaa gcccgtcgtc tccacttcga tccgcgacgt ggtgcgtcct
     1381 tacggcaaac tcggactcgt gcgcatcgcc gacgacgccg cgagctttat acgcgccgcc
     1441 gcagagatcg gcatggacga gcgaacggac gatacgacgt ggctggcgcg cgtcgatgag
     1501 ttcctcgcaa agatctcatg ggatcgcacg tggggcgaga tggccgagtt gatcggcgaa
     1561 gtcgcagcct cgcggcgcgc aaccatcgcg gcgtcggctc agcagtcgaa gcccgccacc
     1621 gtgacgacga ccgttaagca ggcgggcgta gccgcgcgtg cggcgacggc cagcagcagc
     1681 agcgtggcgg ctgccgacta aacaggaaag aatgagagag acaggaatgt tcgattattt
     1741 gatcgtcgga gcagggtatg ccgggagcgt gctggccgag cggctggcac gcgtctccgg
     1801 aaagcgtgtg ctcgtctgtg accgccgccc gcacatcgcc ggcaacgctt acgaccacta
     1861 caacgatgcg ggcgtgctcg tacacaagta cgggccgcat atctttcaca ccaactcgcg
     1921 cgaagttttc gaataccttt cgcagttcac ggaatggcga cagtatgagc atcgcgtgct
     1981 ggcgagcgtg gacgggcaac tgctcccgat cccgatcaat ctcgacacga tcaatcaata
     2041 ttacggtctg aatctgacct cgtttcaggt tgaagaattt ctcaattcag tcgccgaaaa
     2101 gcgcgagcag attcgcacga gcgaggatgt cgtcgtcaat cgcgtcgggc gtgagttgta
     2161 cgaaaagttc ttccgcaact acacgcgtaa gcagtgggga ctcgacccct cggaactgga
     2221 cgcctcggta acggcgcgcg tgccgattag aaccaaccgc gacgaccgct acttcgccga
     2281 cagctatcag gcgatgccgc tgcacggcta cacgcgcatg tttgaaaaga tgctcgatca
     2341 tccgaacatc aagatcatgc tcaacacgga ctatcgcgac atcgagcgca tcatccctta
     2401 caaggagatg atctacacgg gtccggttga cgagtatttc gatttccgtc acggcaagtt
     2461 gccgtatcgc tcgctcgaat tcaaacacga gacgaaggac acggaggttt atcaaagcgc
     2521 gccggtggtg aattatccga acgacaatct ctacacgcgc atcaccgagt ttaaatatct
     2581 gacggggcag gagcatcgca agacgagcat cgtctacgaa tacccgcgcg ccgaaggcga
     2641 cccttattat cccgttccgc gtccggagaa cgcggagctt tacgcgaaat acaaaactct
     2701 cgcggacgag acgccgggcg tccatttcgt cggtcgcctg gcgacttata agtactacaa
     2761 catggatcag gtggtcgcgc aggctctcac gacctacaag aagatcacgg gcgagcgcga
     2821 caaggccaac gtgccgaagt cttaggcact attagccaag gggaaatcga catgagccga
     2881 ggagcaagtc tccctgcccc ggagcttgag ctgtgggctg gcgtggaatg cacggtcaac
     2941 cgcgtcggcg atcagtttta cgatcagctt gagcgcaacg gccacgcccg acggatcgaa
     3001 gacctcgacc tcttcgccac gctcggcgtg cgcgcaatgc gttacccggt tctctgggaa
     3061 cgcaccgcac cgaacggcct cgcgcgcgcc gactggtctt ggcctgatgc gcgcctcgcg
     3121 cgcctgcgcg aactggggat acgccccatc gtcgggctgc ttcatcacgg cagcggccct
     3181 cgccaaacga gcctcgtcga gccggatttt cccactcaac tcgcagctta tgcgcgcggc
     3241 gtcgccgagc gttacccgtg ggtggatgct tacacgccgg tcaacgagcc tctgacgacc
     3301 gcgcgtttca gcggcctcta cgggcactgg tatccgcacg ggcacgacga caaaacgttc
     3361 gtgcgtgcgc tcgtcggcca gtgtcgcgcc accgcgcttg cgatgcgcgc catccgcgag
     3421 atcaacccgc atgcacaact cgtccagacc gaagacctcg gcaaaacttt cagcacgcgc
     3481 acgctccgtt atcaggccga ctttgagaac gtgcgccgct ggatgagcct cgatctgttg
     3541 tgcgggcgca tcacgcgcga gcatcagatg tgggattacc tcacgcgcat cgccgggatc
     3601 ggcgagcggg aactcgaatg gttcaacgcg catccttgcc cgccggatgt tctgggcatc
     3661 aatcattatt tgacgagcga gcgtttcata gatgagcgcg tcgaacgcta cccggatcgt
     3721 gcggtgggcg gcaacgggcg gcatcgttac gtggatgtgg aagcggtgcg cgtctgcgcg
     3781 gaagggacgg cgggggcgcg cgtgctgctg cgcgaggcgt gggagcgtta cggcatcccg
     3841 ctcgcggtga cggaagcgca tctcggctgc acgcgcgagg agcagttgcg ctggctcgga
     3901 gaagtgtggg acgcggcgcg cagcctgcgc gcggacggcg tggatttgcg cgccgtgacc
     3961 gcctggactt tgctcggcgc gtttgattgg tgcagcctgc tcacgcgctg cacgggcaag
     4021 tatgagccgg gcgtcttcga cgtgcgcagc ggcacgccgc gcccgaccgc gctcgcgcgc
     4081 atgctccgcg atctggccga gaagggcgaa cacgatcatc ctgcgctcga ttcgcccggc
     4141 tggtggcggc ggctcgaacg cctgcaatac ccgcccgtgc gtcgccagcc cgcgacaaaa
     4201 gtgtcgacca acaaacaagg actcgatatg agacgtggag acgcgcgacc gctcatcatc
     4261 aacggcgcga cgggcacgct cgggcgcgcg ttcgcgcgtc tgtgccagca gcgcggcctc
     4321 gcttatcgcc tcctgacgcg cgccgagatg gacattgccg atccgcaatc ggtcgaactt
     4381 gcgctcgcgg agtttgaacc gtgggcggtc gtcaacacag ccgggtacgt gcgcgttgat
     4441 gatgccgagc gcgagcctga aaaatgtttc cgggaaaatg gcgaaggcgc ggccacgctc
     4501 gccgccgcat gtgcgcgacg cggcgcggcc ttgctgacgt tttcgtccga cctcgttttc
     4561 gacgggacga aaggaagccc ttacgttgag agcgatgagg tgtcgccgct gaatgtttac
     4621 gggcgcagta aggcggaggc ggaacgtcgt gtgctggcgg agtcgccgtc cgcgctggtc
     4681 ttgcgcacga gcgcgttctt tggtccctgg gacgaacata atttcatcac ggtcgcgctc
     4741 aggacgttgg ccgaagggcg aaagttcgtc gcggcggacg acgcttttgt ctcgccgact
     4801 tacgttccag acctcgtggg ggcgagcctc gatctcttaa tcgacggcga gcgcggcatc
     4861 tggcatctgg cgaacgaggg cgcgatcacc tgggcggaac tggcgcggcg cgcggcgcaa
     4921 cttgccggac tcgatccgca gttggtcgaa gcacgctcga cggtcttcct ccaactctcc
     4981 gcgccgcgcc cgctctacag cgtgctcggc agcgagcgag ggcaactgct cccgacgctc
     5041 gaagatgcgc ttgagcgtta cctgaacgaa cgcgagacgc gctttgcgtc cgtcagcacg
     5101 gcgtcggcag gacaaaagca ggggcgcgcg ggctaaagga aagggatgaa ggatgaggga
     5161 tgagggatga atcagaaaaa gcattcaacc tctcgggcgc atattcttat tcatccctca
     5221 tccctcatcc ttcatccctt catcgccttc atcccttcat cgtatcggca cggtttttga
     5281 ttagtcatct tgaaacatag ttaagctgag ccgttttcgg acagagacgg ggcgcgcggc
     5341 gcgcgtcgtg ttcggaaact gtctggtatt caaaaggaga caagaagatg gcgacaagtg
     5401 agacagcatt gactcaggga cagattgaca ggggcgaagc accgcaaggc tacgcgcggg
     5461 atgtgaacgt gggcgaagtc gaacgctggg cgtcggccat cggcggcggc gcgctcgcga
     5521 tctatggtct gacgcgcggc acgtacggcg gcatcgcgct ggcgttgctc ggcgcaagcc
     5581 ttgtctatcg cggcacgacg gggcactgta acgcgtatga cgcgctgggg atcaacacgg
     5641 cggcgagcga gagagataat ccgttcgtga gcgtcgaggg cgggcgcggg gtgaaggtcg
     5701 aaaagagcgt gacgatcaac aagtcgcccg aagagctata caacttctgg ctcaacttcg
     5761 agaatctgcc gcgcttcatg gaccatctgg aagaggtgcg cttgacgggc gacgggcgtt
     5821 cgcactgggt ggcgaaagga cccgcgggca cgagcgtcga atgggatgcg gagagctaca
     5881 acctgaaaga gaacgagttg atcgcgtggc gttcgctcga agggtcgcaa gtggcaaacg
     5941 caggctcggt tcatttcacg cccgcgccgg gagggcaggg cacggaggtg cgcgtcgtcc
     6001 tgaaatacga tccgcccgca ggcgtcctcg gcgcgtgggt ggcgaaactc ttcggcgaag
     6061 cgcccgagca gcagatcgag gaagacctgc gccgcttcaa aggattgatg gagacgggcg
     6121 agacggcaac caccgccgga caatcctcag gccgcagcgc gaaagcgtaa agacctgtaa
     6181 ccggcaactg aaaaacgttc ctcaggaacg tgtagagagc aaaggaaaag atcaacatgg
     6241 ctgacgaaaa caggagcgag tccggcgcgg gcggcacgca gggcaacgcg tcatccggcg
     6301 gcgcagaaga gcaatcgggc gatccggggc gcacgcccgg cacagccgaa ggtgacgagg
     6361 agacagtcga cgaatcgctc aagcagaaag aatcctagac tttttcgcaa gcccctccgt
     6421 ctaacgctca acgaagagtc gtcgtttcag gcagcctcgc ccgcacgact ctccgttgag
     6481 cgtctctttt tttagttcta ccgatggagt tgaatcctac ttctgcgttt ccggagcaag
     6541 gcttttaaag ggcggcagtc aaagcgaaaa attaaggttt ggcggcggtc gccgtgtggt
     6601 atatttaatc gccctgtcgg aatagtctgc gaagttacct catccgtttg ccctaacaca
     6661 aaaattgacc gcctcacaat tgatttgttg acggtgactc tatgatcgaa ttcacccccc
     6721 gccgcctaca ccctgcgccg tggctgcaac tcgcctgcgc ctgcctcttg atcttcgctg
     6781 ccggtgcttc ttactccgcc ttcgctgcgc ccgtgatttt gaccgaagcg acgacgacgc
     6841 gcgccatcgc cctcgatgcc gtgacgaagt ttcgtgatcc ctttccgctc acgacccccg
     6901 gctacacggc gggcgcagac acgcgcacgc gcatcatgtt cttcgtcctc aatctcgacc
     6961 tcctcgcggg cgagggcgca aacgcgttga ctgccgacgc ggaagacagc gcggggcgca
     7021 agtatgtgtt cgtggtggag aacgtgtcga gcgttccggg ttttgaagcg ttgaagcagg
     7081 tgaccgtgcg cctctcgaac gacgtaggcg acgtgggcga tctcctcgtg cggatcaacc
     7141 tgcacggcgt ggcgagcaat cgcgtccgca tcagcgtcgg ccacgcgggc ggcggcatcc
     7201 cggacgaccc cggctcggtt ccagctcccg cgcccgccgc gcctcccgcg cccacgccga
     7261 ccccgactcc caacccctac accgcagccg ccaccaacgc cgacaccgtc cgtctcctcg
     7321 aacaggccac gtggggagcc acaaacgccg aagtcgcgcg cgtgcagtcg atgggctttc
     7381 gcgcttacct gaacgagcag ttcaacgcgc ccatgtcgag ttatccgaac ctgccgctct
     7441 acccgaccga tcaagcgcaa ggctgtccga cgggcagcgc cgtgacgtgt gggcgcgata
     7501 actattcgat gtacccgctg caagtgaaat tcttccagaa cgcgctggcg acgggcgcgg
     7561 ggtcagacca gttgcgccag cgcgtcgcgt tcgcgctgca tcaggttttc gtcgtctcag
     7621 gccgcgacat caactaccct gcgtggatgg cctactacct gcaaatcctc gaccgcaacg
     7681 ccttcggcaa cttccgcaac gtgctccagg agatcacgct caatccttcg atgggcgagt
     7741 acctgaacat gcgcggcaat cttcggacga accccaacga gaactacgcg cgcgagatac
     7801 tccaactctt ctccatcgga gttgatgaac tgaacctcga cggcacgccc aaactcgacg
     7861 ccgaaggcag gcgcatcccc tcttacagtc aggatacgat caacaatttc gcacgcgtct
     7921 tcacgggctg gaatctggcg gcgaacaaac aggccgtcat caacggcgtc gccatcaacg
     7981 tcttcaatta tcaagacccg atgatcgtct cgcgcgaaac caaccacgac acggcgagca
     8041 agcaactctt gaacggccag attcttccgc ccaaccaaac ttcggcggtg gatttgaacg
     8101 ccgcgctcga caacatcttc aaccatccga acaccgcccc cttcatcggc aagcaactca
     8161 tccagcatct cgtcacgagc aatcccagcc ccgcttatgt ggagcgcgtc gcgcgcgtct
     8221 tcaacaacaa ctgcgacgcc ctctatcctg agagttgcac gaacacgcgc ggcgatttga
     8281 aggcggtcgt ccgtgccgtc ctgctcgacc cggaagcgcg cggcgacgtg aagaccgatc
     8341 cgaagtacgg caaactgcgc gagcctgttc aactcatcac gaacgtcctg cgcgccttca
     8401 acgcgacgag cgacggcgtg cttgcgaaca ggacgacagc ctcaggcggc gacctgccga
     8461 accaactgga tcaaccgctc ttcttacccg cgaccgtttt cagttattac tcgccgacat
     8521 acgaagtgcc cggcgtaaac ctgctcggcc cttcgtttga gattctttcg acttccacct
     8581 cgctcaggcg cgccaacgtc atcaacacgc tcatctatag tggcgtcccg gcctcgccgc
     8641 cgaacgtccc gacgggcacg caaatcaatc tggccgcatt cgaagcgctc gcggccaatc
     8701 cggcgcaact catctctgcg ctcgatgcgt tgctcatgca caacaccctg tccgccgccg
     8761 cgcgcgcgtc catcacgacg gccatcaaca gcatcccgac cacggacgcc aacttcgcgc
     8821 gcaaacgagt tcaggcagcg gcctacctcg tcgccacttc gcatcaatat caggtgcaaa
     8881 ggtaagccgc cgtcagtcgg ccaacagccg tcagctttca cctctcgtcg cacgcaaaag
     8941 ggaatccgtc atggcatcaa cacgcagaga ttttttacgg cagacaggtt gcgcgctcgt
     9001 cggcggcgcg gcgttcgcgt ccggcatcga gcaactcggc ctcatcaacg cgctcgcgca
     9061 gggcagttcg acggcgaccg actacaaagc cctcgtctgc atctttctca gcggcggcaa
     9121 cgacggcaac aataccgtcg tgcccgtcga tgccgcgcgc tacgccgaat atgcgaacgt
     9181 gcgcagcgcc gccggactcg ccatccccgc acccggccaa accggaggct tgttgccgat
     9241 caaccctctg agcggcggcg cgtatggcct ccacccgtcc atgccggagt tgaagacgct
     9301 ctttgacaac cgccaactcg ccgtcgtcag taacgtcggt ccactggtcg aaccgctcac
     9361 gcgcgacacc taccggagcg gcagcggcaa acgtccgctc caactcttct cccactccga
     9421 tcaggtcgcg caatggcaaa cctccgtctc gcacgacgcg agccagacgg gttggggcgg
     9481 tcgcacagcc gaccgcaccg ccacgctcaa cggcgcggcc accttcccgc aggtcgtctc
     9541 catcgcgggc acaaccatct tcgccaccgg agcgaacacg cgcccgctcg gcatctccga
     9601 ctcgcgcacc gctctcacct ccgtcttgcc gctcaacaac ccgccgggcg cagccggtta
     9661 caccgccgcg cagagagacg cgcgccgcgc cgccttcgac cagatgcgca acctcgacct
     9721 cggcgcaacg ctcgtcaagg cttcgcgcga tacgaccgaa caggccatcc aaaccagcat
     9781 cgcgctcgcc acggccaacg ccacgctcgc caccgtcttc cccgatacga cgctcggcta
     9841 ccagttggaa caggtcgcac gtttggtcaa actgcgcgac acgctcagta tcaaacgtca
     9901 aatcttcttt tgcgcgctcg gcggcttcga cacgcacagc gggcagacgg cggccaacgg
     9961 gcagggcgct ttgctcacgc aggtcagcca ggcgatgaag gctttctacg atgcgacggt
    10021 cgaactcggc atacagaaca acgtgacggc cttcacgctc tccgatttcg ggcgcacgct
    10081 gcaacccgca gggacgggcg cgggcgcggt cggctcggat cacggctggg gcaatcacca
    10141 tttcatcatg ggcggcgcgg tgcgcggcgg cgacttctac ggacgcttcc ccacgctcgc
    10201 cctcggcggc cccgacgaca cggacacgcg cgggcgttgg ataccttcaa tctccgtcga
    10261 gcaatacgcc gccacgctcg cctcctggta cggcctcgcg gccagcgacc tgccgctcgt
    10321 cttcccgctc atcgggcgtt tttcgacggc gaacctcggc ttcatgtcgt gaagcgaacc
    10381 ggcgcagaaa ttccatgcga aagatctatc aactcaaact ctgcatcacc gccctcttct
    10441 tcgtcgcagg cgcgggagtt ctcttaacca acaacttcac gactccgtcg gtccacgctt
    10501 tctccgccgg tccacccgcc gggtacacgc gcgcccccgg cgaagaaccg gaatcctgcc
    10561 gcgaatgcca tctcccgcag gaggcgggca cgggcaacat cagcgtcaac gctccgcaaa
    10621 cctacgtccc cggccagact tatcaaatct ccgtcgtcca caccaacaac gatgcgaccc
    10681 gcaggcgttg gggcttccaa ctcaccgcgc tcgacgacac caaccagaaa gcaggcacgc
    10741 tcgcgccgct cgcagacggc ctgacgcagg tgctcaacaa tcaaggaccg tttccgaatc
    10801 gccagtacat cgaacacacc tcgaacggca atttcgacgg ccagaccgga ggcgcgacgt
    10861 ggacgtttaa ctggactgcg ccgcccgccg atgtcggacc cgtcatcttt tacgccgccg
    10921 gaaaccacgc caacggcaac ggcaactccg acggcgatca tatctatttc accttcagca
    10981 ccatcgcccc cgccgctgcc tcgggtgatt tcagcctcaa catcacaccc gcgacacaga
    11041 cgatcctccc cggcgcgagc ggcacttaca ccgtcaccgt cacgccttca aacaacttca
    11101 cgggcacggt caacctgagc gtcctcggtc tgccctccgg cgcgagcgcg acgttcgatc
    11161 cggcgtccgt ccagatcacg gatgccaacg cgcgcagttc gactttgacg atcaacaacg
    11221 gttcgaccac gctgctcggt aatttcacct tgaccgtgaa cggcgcgagc ggcagcttgc
    11281 agcacgcggc ctcggctttc ctcgtcacgg gaccgacgat gttcgacgcg agcctgaacg
    11341 tgcggattct cgcgcgcaac ttgaatcaac cgattgcgct cgcctcaatc ggcgcagacg
    11401 atctcttcgt gctggagaaa gcgtcgggca gagtcctgcg tgtgacgaac ggcgcggcgc
    11461 agagcgcggt gctcgatctc gccgtcaaca gcgcgtccga acgcggcctg ctcggcatcg
    11521 cgctgcaccc gaattttccc gcgaacccgc gcgtctatct ctactggacg gagagcagca
    11581 cgggggcgga cacgaccaac cccgatgaaa cgccgctcct cggcaaccgc gttgacagtt
    11641 ttctctggaa cggcgcgacg ctcacgcccg acaggaattt gatcaaactg cgcgccctgc
    11701 aacaggatgc cgggcagcct tcgcgcggca accacaacgg cggcgtcata cgcttcggac
    11761 tcgacggcaa actctacatc atcatgggcg acaacgggcg tcgcggcctg atgcagaatt
    11821 tgccctttgg tccggcggtc aacgcgcaag gacctctcac agccgacgat caattcggag
    11881 gtcctgaacc tgacaacgcg catctctcgg gcgtcgtcct gcgcctgaac gacgacggca
    11941 cagtgcccgc cgacaatccc ttcttcaacg ccgcgacgaa tctcacggg
//
LOCUS       DASWNS010000548        25508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_203085, whole genome shotgun sequence.
ACCESSION   DASWNS010000548 DASWNS010000000
VERSION     DASWNS010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 25508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25508
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203085"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..612)
                     /locus_tag="VGB76_18860"
     CDS             complement(67..612)
                     /locus_tag="VGB76_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0100994.1"
                     /translation="MSELEAEWAQRLAEAEARARTSGRSDVADYLALRALNDMARNVG
                     IEWLLSIFTAHAGEVNRTGASVIIAHADAHRFRVGNSQMVGRRLTLTAGVRVLTVEAG
                     WPRTPRDGIVRGGGLATARVSHFGSRRDDDELLLVQNAERDAATTTIAPPRWLILDAD
                     GARRAAFEEARARHHVAKLLH"
     gene            745..2625
                     /locus_tag="VGB76_18865"
     CDS             745..2625
                     /locus_tag="VGB76_18865"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0100995.1"
                     /translation="MTYRHVRTMRRGTSLTLSLLLSFVSLAGSAGARQPQPSTRPAAR
                     RADAPRTNTAGQTPARQNQTTAQAARPRLVLVIVVDQFRADYLERFGDLFVPQGGLRR
                     LMRDGAQWTNANYDHMPTYTAPGHATIMTGAWPSETGIVGNDWYEREAGCEVSNASDP
                     EDKHVRDRDRRLCRTEKSRWQLFGGDASERASSPRRLIASTLGDEMRLVTNNRSKVIG
                     ISVKDRGAMLPAGRHANAAYWFSPYTGNMVTSSYYFNELPAWVARYNEARPANKYCGM
                     EWTRLLKDAGEYERRAGADAPPWENIGSANDTNTFPHRIAGSPANASRECWSVLDYSP
                     FSNDLLVQFARTAIENENLGGDADTDVLTVSFSANDYVGHRFGPYSQEAMDITLRTDQ
                     QIGTLLDFVNAKVGLQNTLVAFTADHGVAPIPEHASALGLTGGRIGNTEIMNAARNAV
                     RVRFGRRGEEKDSTADYISESLANANIFFNTVALKRDGINREEIERVVAEAVLTVPGI
                     NRAFTRTQLETGSISPGDVIGRRVLHGFHPRRSGDVVLVQEPFKYFSEGANPIPATHG
                     SPFSYDTHVPLIIMGQGVNAGRYAQAATPADLAPTLSALLRIQAPSNAVGRVLVEGLS
                     NR"
     gene            2686..5364
                     /locus_tag="VGB76_18870"
     CDS             2686..5364
                     /locus_tag="VGB76_18870"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0100996.1"
                     /translation="MKIKKTGLSLCVLIIFASLSATTGATLAQKKTTGRGARPAASTA
                     QPPARGSGARGDEASKAELEALLKLPVAERLERLRAFLAANREPSLRARATEQLVSAH
                     AALGDQRLQTNDAAGGIELFKAAVALAPADMSDRLYYEVVSQLPANLFLRGQASAALE
                     LARAIEPSASSDAKRLLALAAFYISVEQADDAARLARAAITLLPDLAPAHQALGAAHR
                     LALRLDEAAAEYARAVELDPRSTLARHSLADLRRATGKAEEALSLYRELIAADAKNVS
                     ARTGMVLALFDAGKREEAERELEATLTDEPRNLPLLVGAAYWYAAHGETPRALALAEQ
                     AVKIEPRYTWAQIALARALVAGKLPLEAERSLRFARQYGRFPTLDYELASALAATGLY
                     NEAFEELARSFTIKDGQLETQLAGRRPARADSFIELLAPERRASIFQANAADTEANAR
                     VIKGLLALHLALDATNAAGEGSTAANTTPASDSFIAAAANDFTSGTDEMRAFRQLYAA
                     GRLLRRGVALGSVLELMDAARLNIEAAIDAPAATVATTADELRDVRANAIAQGGTPSV
                     PTLPRNAISNILRGRIEELTGMALLGQGKTTEAVTALRRATSVLPEKSLYWRTAQWRL
                     GTALASSGQERDALAAYIKSYNSQSPDPARRAVIESLYTKVNGSPAGLDAQIGSAPTR
                     AAFEATNASRTSSSARTPDAAVTTPTPEPAASATPQPSATPATEPTPEESPLVAAPTP
                     AVTPEATPEATPEATPVTTTPSPEPSPSPASTPFQSNADAEPNAPAASPVEAKRTRVS
                     RNGCALVLSASASELSNSGGSLSVVASLEGNSNLAGISAATTHWSDIIVLREPQGSAD
                     ANSVKFTITSISKTSGNFNVNFKSPCGAQEMIFTVK"
     gene            complement(5495..5962)
                     /gene="trxA"
                     /locus_tag="VGB76_18875"
     CDS             complement(5495..5962)
                     /gene="trxA"
                     /locus_tag="VGB76_18875"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0100997.1"
                     /translation="MAVVMCDNCGAKNRVDEFRAAESLQAVCGRCGAKLTSRGGAQTA
                     QADTGKPVTVTDASFARDVLGSGGRPVLLDCWAEWCGPCRMIAPALDELAAEAQGRYT
                     IAKLNVDQNPRTSAQFGVRSIPTLLIFKDGQLVERIVGAQPKQAIAARLAAHV"
     gene            complement(6202..6594)
                     /locus_tag="VGB76_18880"
     CDS             complement(6202..6594)
                     /locus_tag="VGB76_18880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0100998.1"
                     /translation="MTFINILYAEDFLPVKLIVKETLELEGWRVQDCDDGLTALRQIE
                     SVEQYDLILLDDDLPGLSGVELAARARALGHRSLTPIIMLSATERRNDASAAGVDIFL
                     KKPEGMKSLVETIRQLLSLVGSQRDGFT"
     gene            complement(6841..9120)
                     /locus_tag="VGB76_18885"
     CDS             complement(6841..9120)
                     /locus_tag="VGB76_18885"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0100999.1"
                     /translation="MTFTNLASLQLDDLALPLQDVVRLRCLTAKEFAEAGNYEAACGA
                     LAEWWKHPGERPQVDDLEADSAAEILLRAGVLTGLIGGSKQLSDAQEIAKDLICESIR
                     LFESLGEVEKVGEAQTELGRCYWIMGAYDDARVILSDALKHISPGNSAQRADTLLRSA
                     IVEACATRYSDALRFLTEAAPLFEASDNHALKGPFHMERAIVLEELSTGEHREDYADR
                     ALIDYAAASFHFEQAGHQLFCAKVENNLGFLHYKLARYTDAHEHLNRARQLFTSLNDS
                     NGAAQVDDNRARIFLGLRENAEAERIARAAVKSLENGGQRNLLAETLTTQGTALARLG
                     KRAEARAVLQRAFEVAEQAGDLEGAGVAELTMLEELGDHLTSVELRAIYELADHLLAR
                     TQHPGILERLRASARRVIAAERTHNIEFDTAGFAYGSEQTATLLRGAHAIAYSQGAVL
                     LTGETGTGKELLARLMHEWSGRPGQFVAINCAALSETLLESQLFGHVKGSFTDAVGDY
                     PGAARLAAGGTLFLDEIAELSTANQSKLLRFIEHGEIHTVGAGAPERIDVRVMAATNR
                     VLEDEIAEGRFRRDLFYRLQNFHLELPPLRMRVEDIPAVAEHFIREAMRRQGKRVRFT
                     PDAIEAMCRLPLPGNARELRSLIERTLLAASDGSSIEADAVETVALRLTGKADFADPW
                     ANFSLKEEVQRFEEHLIEKALRDAQGKVSNAARLLGFKHHESLNWRLKNRNKNLLAAR
                     TPAKQRKRSIIKNYQPKRG"
     gene            9269..9520
                     /locus_tag="VGB76_18890"
     CDS             9269..9520
                     /locus_tag="VGB76_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101000.1"
                     /translation="MKRLRQICAVTVLTILLTNVASAEEGISHPWYVPPPTPTPATSA
                     PAQAGSTPTGEANTDNGDSTLDLATEITLQIIQNMLALF"
     gene            9829..11733
                     /locus_tag="VGB76_18895"
     CDS             9829..11733
                     /locus_tag="VGB76_18895"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0101001.1"
                     /translation="MLLRRTSLLSFVWVLLLAAAQVSEAQQRNSRAPVNSSTPEISYT
                     VSMPAPHTHLLQVEMRVRSAGATANLPAQVDLVMPVWTPGSYLVREYARHVQDFAAGE
                     AGGRSLAWSKVNKNTWRVETGGAARELRATYSVYANELTVRTNELHDRHAFWNNSATL
                     MYVEGQLGAPSTVRVVPFGEWKIATGLPAVAGQRNTFRAENFDVLYDSPFLVSDFKTI
                     SFEVRGVPHRIVIDGEGNYDPERMRRDVQKIVEASVEMMGDVPYRDYTFMFMLHPSAG
                     GGLEHLNSTAIIWRRYAFRPEAAWDDFLSLVAHEYFHLWNVKRIRPDALGPFDYTGEN
                     YTRLLWVAEGVTSYYENILSRRAGMISDKEYLTTIANAIRSLQNTPGRLEQSIEEASF
                     DAWIKYYRPDENTLNSAISYYDKGAIVALLLDLEIRRRSSGAKSLDDVLRYLYNEFSK
                     KNRNYTPEDFQRAAEQMAGASLDDFFRRYVRGREELDYNTAFAAVGLQLLTTAPSPAD
                     PSKPAAPRAYFGANLRQDGDRLVVSSVPAGTPAYNQGITFGDQIVALDGGRVTLQTFN
                     ARLEEKRPGDEVRLSVFRLDELRTITIKLGSRVEENYRLVPLAQQTPEQKRLYEGWLY
                     TLGQKSGGGE"
     gene            11813..12073
                     /locus_tag="VGB76_18900"
     CDS             11813..12073
                     /locus_tag="VGB76_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101002.1"
                     /translation="MPKELQREFVSAVQYFLMVLVALVAAFFGVFVPASTGGETVSAI
                     NIGEAWKNIDPYVKSVLFIFSSLSVVRFLFVFLLYSGKKPLA"
     gene            12108..12854
                     /locus_tag="VGB76_18905"
     CDS             12108..12854
                     /locus_tag="VGB76_18905"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0101003.1"
                     /translation="MNFEGQVAIVTGAASGIGRACALEFARGGASGICIVDLAGEDLL
                     TEAARLVREAGARVASFHANVSDYGKAERIVSETRVRLGGAHILVNAAGTTEDAPLWK
                     MTETQWERVLNVNLKGTFNYIRAVAPAFRAQGAGKIVNVASIEALRGRFGISNYAASK
                     AGVIALTRAAARDLGRDGINVNAVAPGFIRTPMVARLPAEVLEEAASETVFGRIGEPE
                     EVATVVAFLCSAAARHITGEVIRVDGGQML"
     gene            13058..13834
                     /locus_tag="VGB76_18910"
     CDS             13058..13834
                     /locus_tag="VGB76_18910"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0101004.1"
                     /translation="MKSYQHIRFTIEDRVGRISLARAPLNVLNITMMREIGAALEECA
                     GVREMVAVVFEAESGARAFSAGVAIEEHVAETIYQMLESFHNIFRALEVMAKPVVALV
                     DGAALGGGCELVAACDIVVASERARFGQPEIKLGVFPPVAAILLPRVIGERRARELIL
                     TGELIDAPEALRLGLVNYVVAPGELEGKAQEILARLRDLSAPALEFARRALDISREER
                     LEEVLARVENLYLNELMKTEDANEGVNAFMEKRKPVWRNR"
     gene            13836..14840
                     /locus_tag="VGB76_18915"
     CDS             13836..14840
                     /locus_tag="VGB76_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101005.1"
                     /translation="MRLFKSRHSRFLGRVTAALSVAVACACLSASVPGARAQEASVRR
                     ETTAARRVDARQEHVERARRAMNIVCAEREQDPQGSAPIDEMQEMPSLPVRHAEVLAG
                     TARAERLLPVAKNLAAESLRALLREHDIKESAAVRAAFARLAQVRVVKPDMELRDNAS
                     VLYREPRTIRFGTIFLAGLRSDEGMISVLAHELTHVADGASGSLNALFRGVAQRASRA
                     AGLQRINLRRGEELTCDLVGARSVRAYITRTKSVETLARRTARALAHNCVERDYTDRA
                     HLSPRTTMRALLTLDPALARDITGEPAQASDVTPNATLRTRRNQRRAAHPVAPRTPRR
                     "
     gene            14889..16241
                     /locus_tag="VGB76_18920"
     CDS             14889..16241
                     /locus_tag="VGB76_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101006.1"
                     /translation="MNKQGNRLKRLSAEGRAPGATPTLLAPRSRRARRAFACVLICAL
                     VSGVGGCRQVGEKLGVSVPRPRELRDVPAARLAFRLETDRDEVVPEAFITDAPEELHA
                     AVKTQFETNRKEQALLRTVVSPAGTRVLALYDAGGGTPSENTFRIDLYGADGMFVRNI
                     LPQNLEGITLAAVAWSPDAQWVAFIGREKPNATPTPTPPDELPLAPPVPTDPNAPAQP
                     TPTVAPLIAPVPVFKTDQIYICDLNGENLRPLTSREGLIYLNLSWAPDSHALAALACR
                     QDELDARLNEGKELAGRPRIIERADGRERLLDDQLMDVQPVWSPDSAKVATAIETDVA
                     IYDALGQSPTGARLPLRDPLFNSSAAYDAAKLKRATAPAGSGKTDSSGGDGSTAATSN
                     SSATPISFNPVISLEWPQPETLLVRTGFVRYYQNSPEPTRSYLRWHLLHLSPQAAVLS
                     "
     gene            16294..16860
                     /locus_tag="VGB76_18925"
     CDS             16294..16860
                     /locus_tag="VGB76_18925"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0101007.1"
                     /translation="MIYQSISDIYAANESVRRQVTARLETLSEAQQTFRPDEGCWSVA
                     QIVEHLAIIEQNMVRLIGMLLNKAEGAAAAPSDDAATPRPFKPFSLDAQVETIRDKKL
                     TAPEQVRPGGQAALADSLASLQRTRATVESFRPRLEAADLEAATYPHPAFGELNISQW
                     LAFIGHHEARHLRQIERLLDAPEFANEN"
     gene            complement(17457..17624)
                     /locus_tag="VGB76_18930"
     CDS             complement(17457..17624)
                     /locus_tag="VGB76_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101008.1"
                     /translation="MLTATAFAAPRLTYRQTEFVVWTFSSLYASAIKSLHLPRHERRG
                     LARDYRLAFAT"
     gene            17866..18996
                     /locus_tag="VGB76_18935"
     CDS             17866..18996
                     /locus_tag="VGB76_18935"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0101009.1"
                     /translation="MSRENSIGIGVIGSGFARRTQIPGFRAATGARVVAIASARRERA
                     EAVAREFAIPFVGDNWRDVVDRADVDLVSIVTPPATHLEMTLYALSRGKAVLCEKPMA
                     LNAAETAQMCRAASAAGVLACIDHELRFLDGRRRMRELLRAGEIGRVRHASVTYRADS
                     RANAERAWDWWSDAKMGGGALGAIGSHVVDGLHWLLGAEVSRIFCQLSTHIIARPVAG
                     ERGDEMRPVTTDDAANLIINFTGGGLLAETGASANASLSFVEAGEPLHRLEIFGECGA
                     LRVEGDGELWRAEMEAGAWRRVETGSAHTVAPGMQDNGWSRGFTRFAQEIVNALLEGR
                     STVEDAATFTDGHRIQLVLDAARRSHASGSWATPDHKGFEAS"
     gene            19232..19447
                     /locus_tag="VGB76_18940"
     CDS             19232..19447
                     /locus_tag="VGB76_18940"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY0101010.1"
                     /translation="MARSIRYTANLIGARHVGLGSDFDGAVAQPFDASGLAQITNALL
                     AEGFGEEEIKMIMGGNVLRVFAEHLPR"
     gene            19498..21537
                     /locus_tag="VGB76_18945"
     CDS             19498..21537
                     /locus_tag="VGB76_18945"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY0101011.1"
                     /translation="MAKRKQKDAGRVVVRVGVDTGGTFTDFVYATRDGLAGMFKLAST
                     PADPSQAITEGLRRIASESGVAVGELEIVHGTTVGTNALLERRGARAALVTTKGFADV
                     LAIGRQARPALYDLNATRPEAIIPRALRFGVRERIAATGEVLEALEESELVELVEKLK
                     RARVESVAVCLLFSFVHPEHERRIARALSKLGVPLSVSHEILPEYREFERTSTVAINA
                     YLQPLMGAYLKRLRAPRLRVMQSSGGSISARAAASEPVRTILSGPAGGVVGALRAALA
                     AGSPDIITFDMGGTSTDVALCAGGQMRTTNEATVAALPVAVPVLDIHTVGAGGGSIAR
                     VDAGGSLRVGPESAGAEPGPAAYGRGTMPTVTDANLVLGRFGGAQLLGGDFKLDARRA
                     HDALERLARELSAASGRRTTAHEAALGVVRVVNAGMERALRVVSVERGFDSRTFALVS
                     FGGAGGLHAVALAESLRIPRVIVPRRPGALSAFGALASDVVLDASRTVMLDAAGGRLS
                     ELERAFQAMERAARTVLSREGFTGERQQRHARSISARYKGQSFELEINYRSRQTLAAA
                     FHRAHLARYGYALAENAVEIVSARLRSRGLVEKLKRERVAQNTTGSKVAPQSTASVYF
                     KTGASRVPVYAREELKAGARLPVPCIVTEYSSTTLVPESARAARIDEQGNLVIEL"
     gene            21563..23320
                     /locus_tag="VGB76_18950"
     CDS             21563..23320
                     /locus_tag="VGB76_18950"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY0101012.1"
                     /translation="MKRTTYDPVTLEIYRALYTSVAEEMGIALRRTAHSPNIKERRDY
                     SCAVFDAGGRVVAQGDHMPVHLGSMPMAVAAALRETGIAPGDVVALNDPFAGGTHLPD
                     VTLVAGVFDVNGSAKGERGERKTRSRTATDASKNSAIENNASADGAIEDDTLENGDAG
                     RSGLDPSPFALSPSPIFYVANRAHHADIGGGSPGSMGMATDVYGEGLRIPPVHLMRGG
                     RMNEDVLRLLLANVRGADERRADFAAQTGSLKTGASRLLEIVARRGVSEAASYASHLI
                     SYSARMMRARIALIPDGVYAAEDALDDDGTSDEPCRVRVRVEIKGARARIDFTGSAPQ
                     VAGPVNAVEAITVSAVAYVFRCLLGAEEVPASAGLMEPLEVVAPPGTIVNALAPAPVA
                     GGNVETSQRIVDVLFKALAQALPELVPAASQGTMNNLTIGGVDQRTGREFAYYETIAG
                     GMGARPAQAGLSAVHTHMTNSLNTPVEALEYAYPLRVREYAMRDASGGRGKHRGGCGV
                     VREIETLVPARMALLADRRRRAPYGLAGGAEGRAGHDSVIGGDGRKRRIKSKGSHELK
                     AGDRIRIETPGGGGHGKKR"
     gene            23537..25225
                     /locus_tag="VGB76_18955"
     CDS             23537..25225
                     /locus_tag="VGB76_18955"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY0101013.1"
                     /translation="MRVTIGQINTTNGDYEGNVARILHAIERARAEGSDLVVLPEVAV
                     QGYMSFDWFLDRDVVAHAMEPLDEIIAASAGLTVIVGTVRPTGQTGGRRLYNSAAVIS
                     DGKLLGFADKTLLPEYDVFDDPRYFEPAKERRLFDIEGMRVGIAVCEDMWNDKTYWRE
                     RLYANDPTDDLIALGAQLIVSINASPFNKSKMGQRCSMVSHRAKAAGVPIVFVNLVGA
                     NDGVIFDGASIVTDRKGQIILQAPPFEEFYGSVDLDCDQCDTYCLPGDDIQTVHDALV
                     LGIRDYARKNRFERAVLGLSGGIDSALVAALACEALGAANVLSVMMPSPFSSRGSVDD
                     SVALGLNLGMPTIEHPISAAYEVLVKQLKLENPGTGQEELARQNTQSRLRGNILMAIS
                     NAEGRLLLSTGNKSELALGYCTLYGDTNGGLAVLGDVLKTEVWALSRYLNREREIIPR
                     AIIEKRPSAELAPGQFDDQSLPPYDKLDPILKLYFELKATPEEIVAKGHDQQLVFDVL
                     NRVEAPANEFKRRQLPPTLIISRHAIGIGRRRPVTHRYRRQVLKAVERAAEGQG"
BASE COUNT         4816 a         8014 c         8012 g         4666 t
ORIGIN      
        1 gagagtttct aactggcggt cagagacggc gtggagagtc tggccggaca attctatttg
       61 tgaagatcaa tgcaggagct tggcgacgtg gtggcgtgcg cgcgcttctt caaacgcagc
      121 ccgccgcgcg ccgtctgcat cgaggatcaa ccagcgcggc ggcgcaatag tagtagtggc
      181 cgcgtcgcgc tcggcgtttt gcacgagcag gagttcgtcg tcgtcgcgtc ggctgccgaa
      241 gtgagagacg cgcgccgtgg cgagtccgcc gccgcgcacg atcccgtcgc gcggcgtgcg
      301 cggccaaccg gcctcgacgg ttaagacgcg cacgcccgcc gtcagcgtca ggcggcgtcc
      361 gaccatctgc gaattgccga cgcgaaaacg gtgtgcgtcc gcgtgcgcga tgatgacgct
      421 cgcgcccgtg cggttaactt cgcccgcgtg cgccgtgaag attgagagca gccattcgat
      481 gccgacgttg cgcgccatgt cgttcaacgc gcgcagggcg agatagtcgg ccacgtcgct
      541 gcgcccgctc gtgcgcgcac gcgcctcggc ctcggccaga cgctgcgccc attccgcttc
      601 aagttcgctc aactgtctac tcgcttcaaa tgattcgtcg cttgccttca acggacttgc
      661 gccgtgatgg tatcatgccc gcacgctcga aagttaatgt acgcctgagg tcaggctccc
      721 gacctgttta caaagaggca cttgatgaca taccggcacg tccgcacaat gcgtcgcggc
      781 accagcttaa ctctctcgct gctcctctcc ttcgtctccc tcgccggaag cgcgggggca
      841 cggcagccgc aaccgtccac gcgccccgcc gcgcgccgtg ccgatgcgcc gcgcacaaac
      901 accgccggac agacgcctgc gcgccagaac cagacgacgg cgcaggccgc gaggccgcgt
      961 ctcgtgctcg ttatcgtcgt cgatcaattt cgcgcagact atctcgaacg cttcggcgat
     1021 ctcttcgtcc cgcagggcgg actccgccgt ctcatgcgcg acggcgcaca gtggaccaac
     1081 gcgaactatg atcacatgcc gacttacact gcgccgggac acgccaccat catgaccggc
     1141 gcgtggcctt ccgagacggg catcgtcggc aatgactggt acgagcgcga ggcgggatgc
     1201 gaagtgtcga atgcttccga ccccgaagat aagcacgtga gagataggga cagaaggctg
     1261 tgccgtaccg aaaaatcgcg ctggcaactc ttcggcggcg acgcgtccga gagggcttcc
     1321 agcccgcgcc gcctgatcgc ttcgacgctc ggcgacgaga tgcgtctggt gaccaacaat
     1381 cgctcgaaag tgatcggcat ctccgtcaag gatcgcgggg cgatgctgcc cgccggtcgt
     1441 catgccaacg cggcctactg gttcagcccg tacacgggca atatggtcac gagcagttac
     1501 tacttcaacg aactgcccgc gtgggtggcg cgttacaacg aagcgcgtcc ggcgaataaa
     1561 tattgcggga tggaatggac gcgcctgctc aaggacgcgg gcgaatacga gcgacgtgcg
     1621 ggagcggacg cgccgccgtg ggaaaacatc ggctccgcca acgacacgaa cactttcccg
     1681 cataggatcg cgggcagccc ggcgaatgcg agccgcgaat gttggagcgt gctcgactat
     1741 tcgcctttct cgaatgacct gctcgtgcag tttgcacgga cggcgattga gaatgagaac
     1801 ctgggtggcg acgccgatac ggacgtgctc acagtgagtt tctccgccaa cgattacgtg
     1861 gggcatcgct tcggaccata cagtcaggaa gcgatggaca tcacgttgcg cacagaccag
     1921 cagatcggga cgttgctcga tttcgtcaac gcgaaagtcg gcctgcaaaa cacgctcgtg
     1981 gcgttcaccg ccgatcacgg cgtcgcgccg attcccgaac acgcttccgc gctcgggctg
     2041 acgggcgggc gcatcggtaa cacggagatc atgaacgccg cgcgcaacgc cgtccgcgtg
     2101 cgcttcgggc gcagaggcga ggagaaggat tccaccgccg actacatctc cgagtcgctg
     2161 gcaaacgcca acatcttctt caacacggtg gccttgaagc gcgacggcat caaccgcgaa
     2221 gagatcgagc gcgtggtggc ggaagccgtc ctgaccgtgc cgggcatcaa ccgcgcgttc
     2281 acgcgcacgc aactggagac gggcagcatc tctcccggcg acgtgatcgg gcggcgcgtg
     2341 ctgcacggct tccacccgcg acgcagcggc gacgtggtgc tcgtacaaga gcctttcaaa
     2401 tatttcagcg aaggcgcaaa cccgatcccc gccacgcacg gttcgccctt ctcttatgac
     2461 acgcacgttc cgctcatcat catggggcag ggcgtcaacg caggtcgtta cgcgcaggcc
     2521 gcgacgcccg ccgaccttgc gccgacgctc tccgcgctcc tgcgcataca ggctccgagc
     2581 aacgccgtcg ggcgcgtcct tgtcgaaggc ttgagcaaca ggtaaaactt tgcccggcgt
     2641 cagaagtaga agcggagtag aagtgaagta gaagtgaagt ttgttatgaa gattaaaaaa
     2701 acgggtttga gcctttgtgt gctgatcatt ttcgcgtctc tttcagcgac gaccggcgcg
     2761 acgctggcgc agaagaagac gacgggtaga ggcgcacgcc ccgccgcctc gacggcgcaa
     2821 ccgcccgcgc gcggttcggg cgcgcgtggg gatgaagcgt cgaaggccga actcgaagcc
     2881 ctgctcaaat tgcctgtggc cgaacgcctt gagcggctgc gcgcgtttct cgcggccaac
     2941 cgggagccat cgctcagggc gcgtgccacg gagcaactcg tgagcgcgca cgctgcgctc
     3001 ggcgaccagc gtttgcagac caatgatgcg gcgggcggga tcgaactctt caaagcggcg
     3061 gtcgcgcttg cgcccgcaga catgtccgac aggttgtact acgaagtcgt ctcgcagttg
     3121 cccgcgaatc ttttcctgcg cggtcaggct tcagccgcgc tcgaactcgc acgcgcgatt
     3181 gaaccgtcgg cgtcgtcgga tgcaaaacgt ctgctcgcgc tcgcggcctt ttacatcagc
     3241 gtcgaacagg cggacgacgc cgcgcggctg gcgcgtgccg ccatcacgct cctgcccgat
     3301 ctcgctccgg cgcatcaagc tctcggcgcg gcgcaccggc tcgcgctccg gctcgacgaa
     3361 gcggcggcgg aatacgcacg cgccgtcgaa ctcgacccgc gctcgacgct tgcccgccac
     3421 agtctcgcgg atttgcgacg cgccacgggc aaagccgaag aagctctgtc gctctatcgt
     3481 gagttaatcg cggcggacgc gaagaatgtt tcggcgcgca cggggatggt gctcgcgctc
     3541 ttcgatgcgg gcaagcgaga agaggccgag cgcgaactcg aagccacact cacggacgag
     3601 ccgcgcaatt tgccgctcct cgtcggcgcg gcttactggt acgcggctca cggtgagacg
     3661 ccgcgcgcac tcgcgttggc ggaacaggca gtgaagattg aaccgcgtta cacttgggcg
     3721 cagatcgcct tagcgcgcgc tctcgtcgcc ggcaaactgc cgctcgaagc cgaacgctcc
     3781 ctgcgcttcg cgcgccagta cggacgtttc ccgacgctcg attacgaact ggcgagcgcg
     3841 ctcgccgcga cgggtctgta caacgaagcg ttcgaggaac tcgcgcgcag tttcacgatc
     3901 aaagacggcc agctcgaaac tcaactcgcc gggcgcagac ccgcacgcgc cgacagtttt
     3961 atcgaactgc tcgcgcccga acgccgcgcc agcatctttc aggcaaacgc cgctgacacc
     4021 gaagcgaacg cgcgcgtgat caaaggtctg ctcgcgctcc atctcgcgct cgatgcgacg
     4081 aacgcagcgg gcgagggaag cactgcggcg aacacgactc cggcgtccga ttcattcatc
     4141 gctgccgctg ccaatgattt cacttcgggc acagacgaga tgcgcgcttt tcgtcagctc
     4201 tacgcggcgg gtcgcctgct tcgtcgcggc gtcgcgctcg gaagcgtgct tgagttgatg
     4261 gacgcggcgc gtctcaatat cgaagccgcg attgacgcgc ccgccgcgac cgtcgcaaca
     4321 accgcagacg agttgcgcga cgtgcgcgcg aacgccatcg cgcagggcgg cacgccgagc
     4381 gtccccacgc ttccgcgcaa cgccatctcg aatattctgc gtggccggat cgaagaattg
     4441 acaggcatgg ctctgctcgg acagggcaag acgacggaag ccgtgaccgc gctccgccgc
     4501 gcgacgagcg tgctgccgga aaagagtctc tactggcgca cggcgcagtg gcggctcgga
     4561 accgcgctcg ccagcagcgg gcaggaacgc gacgcgctcg ccgcctacat caagagctac
     4621 aattcgcagt cgcccgatcc ggctcgccgc gccgtgatcg aatcgctcta tacgaaagtc
     4681 aacggctcgc ccgccggact cgacgcgcaa atcggctccg cgcccacgcg cgccgccttc
     4741 gaagcgacga acgccagccg cacctcttcg tccgctcgga cgcccgacgc cgctgtcaca
     4801 acgccgacgc ccgaacctgc cgcttccgcg actccgcaac cttcggccac tccggccact
     4861 gaacccacgc cggaggaatc acccctcgtc gccgccccga cgcccgccgt gacgcccgaa
     4921 gcgacgccgg aagcaacgcc cgaagcgacg cccgttacta caacgccgtc tccagagcct
     4981 tcgccttctc ccgcctcgac gccgtttcaa tcaaacgccg acgcggaacc gaacgcgcca
     5041 gccgcgtcgc ccgtcgaagc gaagcgaacg agagtgagcc ggaacggctg cgcgctcgtg
     5101 ttgagcgcga gcgcgtcgga gttgagcaac agcggcggct cgctgagcgt cgtggcaagc
     5161 ctcgaaggca acagcaatct cgcgggcatc agcgcagcca ccacacactg gtcggacatc
     5221 atcgtgctac gcgaaccgca aggcagcgca gacgccaact ccgtcaagtt cactatcact
     5281 tccatcagca aaaccagcgg caacttcaac gtcaacttca aatcgccctg cggcgcgcaa
     5341 gagatgatct ttacggtgaa atgaaagcgg ccaaaggggc agcggtcaag ggttaagacg
     5401 ggcaaagata aaagcggagg cagcttgcga gggctgcgct ccgcttctct ttgcctgtcc
     5461 tttttttacc tcttccctct cttctccctc cctttcagac atgcgcggcg aggcgtgcgg
     5521 cgatagcctg tttgggttgc gcgccgacga tgcgttcgac gagttggccg tctttgaaga
     5581 tgagcagggt gggaatgctg cggacgccga actgcgcgga ggtgcgcggg ttttgatcga
     5641 cgttcaattt ggcgatggtg tagcggcctt gcgcttcggc ggcgagttcg tcgagcgcgg
     5701 gcgcgatcat gcggcacggc ccgcaccatt cagcccagca gtcgagtaac acagggcggc
     5761 ctccgctgcc gagcacgtcg cgcgcgaaac tggcgtccgt taccgtgacg ggcttgcccg
     5821 tgtccgcctg cgccgtctgc gctccgccgc gcgacgtcaa cttcgcgccg caccgcccgc
     5881 agacggcctg caaactctca gccgcccgaa actcgtccac acgattcttc gcgccgcagt
     5941 tatcacacat cacgactgcc atactccctc aacgcctccg ttcttcaatt tgcgtgaagg
     6001 gaaattataa cgggcgcggc gtgaaagaaa aaagcagcgc ggtgcgcgtg aagcggcgcg
     6061 cgtggataag agagattcgc agcgcgaaaa tttcgtcgcc gtcgtaagtt aactatgctt
     6121 caaagctaac cgcgctctaa ttttgtccgg gtcgtgagtt gcagctgccg ccgggtgttc
     6181 tctcagcttc gctctccgct tttatgtgaa accgtcccgt tgagagccga cgagggagag
     6241 cagttgcctg atcgtctcga cgagcgactt catgccttcg ggtttcttca ggaaaatatc
     6301 tactccggct gcgctggcat cgttcctgcg ttcggtggcc gagagcatga tgatgggcgt
     6361 gagactgcga tgtccgagtg cgcgtgcgcg cgcggcgagt tcgacgccgc tgagtcccgg
     6421 caggtcgtcg tcgagcagga tgagatcata ttgctcgacg ctctcgatct ggcgcaacgc
     6481 cgtgagtccg tcgtcgcagt cctgcactcg ccagccttcg agttcgagcg tctctttaac
     6541 gatgagtttg accggcagaa aatcttccgc gtagagaatg ttaatgaaag tcattcgggg
     6601 ctgacatcct ttagtgagaa tccggacgca cgcgcagccg ccgctgtagt aatcaccggc
     6661 ggcggtagag agtcggagtt tacaggcgcg agccttgtcg tcggaacacg agccggtaac
     6721 acgatgaggc agccgtgtta caagtttctc tatgcaggaa tcaggccaat gcgaagggag
     6781 gccgttttac ggcgcatccg gacgctgtct ggggtgggcg acgtggaaaa tttagaggcg
     6841 ttatccacgt ttcggttgat agttcttaat gatacttcgc ttgcgctgct tggcgggggt
     6901 tctggccgcg agcaggttct tgtttctgtt tttgagacgc cagtttagag attcatggtg
     6961 tttgaagccg agcagccgcg ccgcgttaga caccttgcct tgcgcgtcgc gcagagcctt
     7021 ctcgatcagg tgttcttcga aacgctgcac ctcttctttg agcgagaagt ttgcccaggg
     7081 gtctgcgaag tcagccttgc ccgtcaagcg cagcgcgact gtctccacgg cgtcggcttc
     7141 gatgctgctc ccgtcagacg ccgccagcag cgtgcgctcg atgagcgagc gcaactcgcg
     7201 cgcgttgccc ggcagaggca gacggcacat ggcctcgatg gcgtcgggcg tgaagcgcac
     7261 gcgcttgccc tgtcgcctca tcgcttcgcg gatgaagtgc tcggcgacag ccggaatatc
     7321 ttcgacgcgc atacggagcg gcggcagttc gagatggaaa ttttgcagac gatagaagag
     7381 atcgcggcgg aaacgtccct ctgcgatctc gtcttcaagg acgcgattcg tcgccgccat
     7441 tacgcgcacg tcgatgcgtt cgggtgcgcc cgcacccacc gtatgaatct cgccgtgctc
     7501 gatgaagcgc aggagcttgc tttgattggc cgtgctcagt tcggcgatct cgtccaggaa
     7561 gagcgtccca cccgccgcga gtcgtgctgc gcccggataa tccccaaccg catccgtgaa
     7621 actgcccttg acatggccga acaactgcga ctccagcaac gtctccgaga gcgccgcgca
     7681 gttgatggcg acgaactggc cggggcgacc gctccactcg tgcatcaggc gcgcgagcaa
     7741 ctccttgccc gttcccgtct cgcccgtcaa cagcaccgcg ccctgtgaat aggcgatggc
     7801 gtgcgcgcct ctaagcagag tcgccgtctg ctcggagccg taggcgaagc ccgccgtgtc
     7861 aaactcgata ttgtgcgtgc gctcggcagc gatcacacga cgcgcgctgg cgcgcagacg
     7921 ttccaaaatc cccggatgct gcgtccgcgc caacaggtga tcggccaact catagatggc
     7981 gcgcaactcc acgctcgtca agtgatcgcc caactcttcg agcatcgtca gttcggcgac
     8041 gcccgcgccc tctaggtcgc ccgcctgttc cgccacctca aacgcgcgct gcaacacggc
     8101 acgcgcctcc gcacgctttc cgagtcgcgc cagagccgtc ccctgcgtcg tcaaagtctc
     8161 ggccagaagg tttcgctgcc cgccgttctc cagagactta acagcagcgc gcgcgattct
     8221 ttcggcctcc gcgttctccc tgaggccgag gaagatgcgc gcacggttat cgtccacctg
     8281 cgccgcgccg ttgctatcgt taagactcgt aaatagttgg cgcgcgcgat taagatgctc
     8341 gtgggcgtct gtgtacctgg ctaacttgta atgtaaaaat ccgaggttgt tttcaacttt
     8401 ggcgcaaaaa agttggtgtc ccgcctgctc gaaatgaaaa ctcgccgccg cataatcgat
     8461 aagcgcacga tctgcatagt cctcccggtg ctcgcccgtg ctaagttctt cgagcacaat
     8521 ggcgcgttcc atgtggaaag gcccttttag cgcgtgatta tcgctggctt cgaacaaggg
     8581 ggcggcttcg gtcaaaaagc ggagcgcatc gctgtaacgt gtggcgcaag cctcgactat
     8641 cgcgctgcgc agcagtgtat cggctctctg cgcgctgttg ccgggcgaaa tatgcttcaa
     8701 tgcgtcgctc agtatcacgc gggcgtcgtc gtaggctccc atgatccagt agcaacgacc
     8761 gagttccgtc tgcgcttccc cgactttttc aacttctccc agtgactcaa aaaggcggat
     8821 actttcgcag atcaagtctt tggcgatctc ctgggcgtcc gaaagttgtt tggaaccacc
     8881 gattaaccct gttaacacgc ctgcgcgtaa caggatttcc gccgcgctgt cggcttctaa
     8941 atcatcaact tgcgggcgtt cgccgggatg cttccaccat tcggccagtg ccccgcaggc
     9001 cgcctcgtag ttgcccgctt ccgcaaattc tttagcagtc aggcatcgaa ggcggactac
     9061 atcctggagg ggcagggcta aatcgtccag ttggagagat gctagatttg taaaagtcat
     9121 gtaatcatta cctttgtaag tcactgctaa ttaacttgca agcagttaaa cctttgccta
     9181 tgatgcttct ggattgtttt agtctggcct ttaaatcacg gtatgttatc ggctaccgct
     9241 gatcgcaacc cttcttttag gagaaaccat gaaacgctta cgtcaaatct gcgctgttac
     9301 tgtcttaact attttgctca ctaatgttgc atcggctgaa gagggtattt ctcacccctg
     9361 gtatgtgccg ccgccgacgc cgacgcccgc cacttcggca cctgcgcaag ccggttctac
     9421 tcccactggc gaagcaaata cagataacgg ggactcgaca ctcgatcttg cgacggagat
     9481 tacgctacag attatccaga acatgctcgc attgttctaa aaaatatctc tccgtcatcg
     9541 agcctaaaaa cgctgctgtg atttatctca ggcggcgcgc tcttcgtgtg gcgtgaagac
     9601 gtggcctgcg ggtcacgaca gatgcccgga gagaggagac atccgctgca cggcgaagat
     9661 taactgttaa agcatattaa atgcaattgt tttcttcgtt ttggacggat aaaaagtttc
     9721 cgcgcgggaa cgggggaatg gtcgtcgtgt cggctttcgt agtaagatgc gagatcagaa
     9781 ggcttctccc gtacatcttc ggcctaagaa aaggaaagtt gctcaacgat gcttctacgt
     9841 cgcacgtctc tgctgtcgtt cgtgtgggtg ctgctcttgg cggcggctca agtttctgag
     9901 gcgcagcagc gaaacagccg cgctcccgtt aacagttcta cgccggagat ctcttacacc
     9961 gtctccatgc ctgcaccgca cacgcatctc ttgcaggtcg agatgcgcgt gcgcagcgca
    10021 ggcgcgacgg cgaatcttcc cgcgcaggtt gatctcgtga tgcccgtctg gacgccgggg
    10081 tcgtatctcg tgcgcgagta tgcgcggcat gtgcaggatt tcgcggcggg cgaggcgggc
    10141 ggcagatcgc tcgcgtggag caaagttaat aagaacactt ggcgcgtgga gacggggggg
    10201 gcggcgcgtg agttgcgcgc cacttacagc gtctatgcca acgaactgac ggtgcggacg
    10261 aacgagttgc acgaccgtca tgctttctgg aacaactccg cgacgctcat gtacgtggag
    10321 ggtcaactcg gcgcgccttc gacggtgcgc gtcgtgccgt tcggcgaatg gaagatcgcc
    10381 accggcctgc ccgccgtcgc agggcagcgc aacactttcc gcgcagagaa cttcgacgtg
    10441 ctctacgact cgccctttct cgtcagcgac ttcaagacga tctccttcga ggtgcggggc
    10501 gtgccgcacc gcatcgtcat tgacggcgag ggcaactacg acccggaacg catgcgacgc
    10561 gacgtgcaga agatagtgga agcgtcggtc gagatgatgg gcgacgtgcc gtatcgcgat
    10621 tacactttca tgttcatgct ccacccttcg gcgggtggcg gcctcgaaca cttgaactcg
    10681 acggcgatca tctggcggcg ttacgctttt cgtcccgaag cggcctggga cgattttctc
    10741 tcgctcgtcg cgcacgaata ttttcatctc tggaacgtta agcgcatccg acccgacgcg
    10801 ctcggccctt tcgattacac gggcgagaac tacacgcggc tactgtgggt ggccgaaggc
    10861 gtcacgagtt attacgaaaa cattctgtcg cgccgcgccg gaatgatctc ggacaaggaa
    10921 tatctgacga cgatagccaa cgcgatacgc agtttgcaga acacgcccgg acggcttgaa
    10981 caatcaatcg aagaggcgag cttcgacgcg tggatcaagt attaccgccc cgacgagaac
    11041 acgctcaact cggccatctc ctattacgac aagggagcca tcgtcgccct cctcttagac
    11101 ctcgaaatcc gtcgccgctc atcgggcgcg aagtcgctcg acgacgtgtt gcgttatctc
    11161 tacaacgagt tttctaaaaa gaaccgcaac tacacgcccg aagattttca acgcgcggcg
    11221 gaacagatgg cgggcgcgag cctcgacgat ttcttccgcc gctacgtgcg cgggcgcgaa
    11281 gaactcgact acaacacggc ctttgccgcc gtcggcctgc aactcctgac gaccgccccc
    11341 tcgcccgccg acccgtcgaa acccgccgcg ccgagagcct acttcggcgc gaacctacgg
    11401 caggacggcg accgcctcgt cgtctcaagc gtcccggcgg gcacgcccgc ttacaatcag
    11461 ggcatcactt tcggcgatca gatcgtcgcg ctcgacggcg ggcgcgtcac gctccaaacc
    11521 ttcaacgcgc ggctcgaaga gaagcgaccg ggcgacgaag tgcgtctctc tgtctttcgt
    11581 ctcgacgagt tgcgcacgat cacgatcaaa ctcggcagcc gcgtcgaaga aaactaccgc
    11641 ctcgtgccgc tcgcgcagca gacgcccgaa cagaagcgac tctatgaagg ctggctctac
    11701 acgctcgggc aaaaaagcgg cggcggcgaa tagacaatcc gccttagaga cgttgaacct
    11761 cgcggaacag cagggctgaa gcgttgtcta agccttgttg gaggtttagt ctatgccgaa
    11821 agagttgcag cgcgagttcg tgtctgctgt tcaatacttc ctgatggttc tcgtcgctct
    11881 ggtcgcggcc ttctttggag tctttgtgcc tgcttcaacg ggcggggaga cggtgtccgc
    11941 gataaatata ggggaagcgt ggaagaatat agacccgtac gtgaaatccg tactgttcat
    12001 cttctcgtcg ttgagcgtcg tgagatttct gttcgtcttt ctcctttatt caggtaaaaa
    12061 gcctctggct taaaatatcc tcagatgaac gaagcactga tgcgctgatg aacttcgagg
    12121 ggcaggtagc aatcgtcacg ggcgcggcct ccggcatcgg tcgcgcgtgc gctctggagt
    12181 ttgcgcgtgg cggggcgagc ggcatctgca tcgtcgatct cgccggagag gatttgctga
    12241 cggaggccgc gcgcctggtg cgagaagcgg gcgcgcgggt ggcgagcttt cacgctaacg
    12301 tctcggacta cggaaaagcc gaacgcatcg tctcggagac gcgcgtgcga cttggcggcg
    12361 cgcacatcct cgtcaatgcc gcagggacga ccgaagacgc gcccctctgg aagatgaccg
    12421 agacgcaatg ggaacgcgtg ctgaacgtga acctcaaagg gaccttcaac tacatacgcg
    12481 ccgttgcgcc cgcgtttcgc gcgcagggcg cggggaagat cgtcaacgtc gcttccatcg
    12541 aagcgttgcg cggcaggttc ggcatcagca actacgccgc ttcaaaagcc ggagtcatcg
    12601 cgctcacacg cgcggcggcg cgcgatctgg ggcgcgacgg gatcaacgtc aacgcggttg
    12661 cgcccggctt catacgcacg ccgatggtgg cacgcctgcc cgcagaagtt cttgaagaag
    12721 ccgcgagcga aaccgtcttc gggcgcatcg gcgaaccgga agaggtcgct acggtcgtcg
    12781 cctttctctg ctccgccgcc gcacgccaca tcacgggcga agtgatccgc gtcgatggcg
    12841 gccaaatgct ttgagaacag tgacgggtga cgggtgacgg gttaaaatct cgtcgcttgc
    12901 tgctagtcgc ttccggctcg ttgctgcttg ttcgttgcgt tttggtcggt gcttcttgtc
    12961 catcatttgt cgcgcacacg aaagtcagaa acgacggcaa cacttttaac cggttccggc
    13021 tcgtcacttg tcactcggca cttgtcactg ttctcttatg aaaagctacc agcatatccg
    13081 ttttaccatc gaagaccgcg tggggcggat cagtctggcg cgtgcgcctt tgaatgttct
    13141 gaatatcacg atgatgcgcg agatcggggc ggcgcttgag gagtgtgcgg gtgtgcgcga
    13201 gatggtggcg gtggtgttcg aggctgaaag cggcgcgcgt gcattcagcg cgggcgtggc
    13261 gattgaggag catgtggcgg agacgatcta ccagatgctc gaatcgtttc ataacatctt
    13321 ccgcgcgctg gaggtgatgg cgaagcccgt cgtcgcgctc gttgacgggg ctgcgctcgg
    13381 cggcgggtgc gagttggtgg cggcgtgcga catcgtggtc gcgtcggagc gtgcgcgttt
    13441 cgggcagccg gagatcaagt tgggggtctt tccgccggtg gcggcgatcc tgttgccgcg
    13501 cgtgatcggc gagcgacgcg cgcgcgaact catcctgacg ggcgagttga tcgatgcgcc
    13561 ggaagccttg cgcctcggcc tcgtcaacta cgtcgtcgcg cccggcgaac tcgaagggaa
    13621 ggcgcaggag atactggcgc gcctgcgtga tctctccgcg cccgcgctgg agtttgcgag
    13681 gcgtgcgctc gacatctcgc gggaagagcg tctggaagag gtgctggcgc gcgtcgagaa
    13741 tctctacctg aacgagttga tgaaaacgga agacgccaac gaaggcgtca acgcctttat
    13801 ggagaaacgc aaacccgtct ggcgcaatcg ttgatatgcg attattcaag tctcgccata
    13861 gtagattttt agggcgggtg acggctgcgt tgtcggtcgc cgtcgcctgc gcgtgcctgt
    13921 ctgcgagcgt gccgggcgcg cgtgcgcagg aggcgagcgt tcgccgggag acgacggcgg
    13981 cgcggcgcgt agatgcgagg caggagcatg tggaacgcgc gcggcgtgcg atgaatattg
    14041 tgtgcgcgga gcgcgagcaa gacccgcagg gcagcgcgcc gattgacgag atgcaggaga
    14101 tgccttcgct gcctgtgcgt catgcggaag tgctggcggg cacggcgcgc gcggagcgtc
    14161 tgctgcccgt ggcaaaaaat ctggccgccg agtcgctgcg cgcactcctg cgcgagcacg
    14221 acatcaagga gtctgccgcc gtccgtgcag ccttcgcgcg gctcgcacag gtgcgcgtgg
    14281 taaagcccga catggaactg cgcgacaatg cttcggtgct ctacagagaa ccgcgcacca
    14341 tccgtttcgg caccatcttt ctcgccgggc ttcgttcgga cgaaggcatg atcagcgtgc
    14401 tcgcgcacga actgacgcac gtggccgacg gcgcaagcgg cagcctcaac gcgctctttc
    14461 gcggcgtcgc gcagagggcg tcgcgcgccg caggtctaca gcggatcaat ctgcggcgcg
    14521 gcgaagagtt gacgtgcgat ctcgtcggcg cgagaagcgt gcgcgcttac atcacgcgca
    14581 cgaagagcgt ggagactctc gcacggcgca cggcgcgcgc cctcgcgcac aactgcgtcg
    14641 aacgcgacta cacggatcgc gcgcaccttt cgccgcgcac gacgatgcgc gctctgctca
    14701 cgctcgaccc cgcgctcgcg cgagacatta cgggcgaacc ggcgcaggcg agcgacgtga
    14761 cgccgaacgc gacgctgcgc acgcgccgca accagcgacg cgccgcgcac ccggttgcgc
    14821 cgcgcacgcc gcgtcgttga cagagtcgca cgcggcttcc tatcattttc gtattcaaga
    14881 tgaataagat gaacaagcag ggaaatcgtt tgaaacgtct gtccgcagag gggcgcgcgc
    14941 ccggcgcgac tccaacactg ctcgcgcctc gaagccgtcg tgcgcgtcgc gcgttcgcct
    15001 gcgtgctcat ctgtgcgctc gtgtcgggcg tgggcgggtg caggcaggtg ggcgagaagt
    15061 tgggcgtgag cgttccgcgc ccgcgcgagt tgcgcgacgt gcccgccgcg cggctcgcct
    15121 ttcgtctgga gactgatcgt gacgaggttg tgcccgaagc ctttatcacg gacgcgcccg
    15181 aagaactgca cgccgccgtg aagacgcagt ttgagacgaa ccgcaaagag caggcgctcc
    15241 tgcgcacggt cgtctcaccg gcgggcacgc gcgtgctcgc gctctacgac gcgggcggcg
    15301 gcaccccctc cgaaaacact tttcgcatag acctctacgg cgcagacggc atgttcgtgc
    15361 gcaacatcct gccgcaaaat ctggaaggca tcacgctggc tgcggtcgca tggtcgcccg
    15421 atgcgcagtg ggtcgcattc atcggacggg agaagccgaa cgcgaccccg acgcccacgc
    15481 cgcccgacga acttcccctc gcgccgcccg tccccacaga cccgaacgcc ccggcgcaac
    15541 cgacgccgac cgtcgcgccg ctcatcgcgc ccgtgcccgt cttcaagacc gatcagattt
    15601 acatctgcga cctcaacggc gaaaatttgc gcccgctcac ctcgcgcgaa ggtttgatct
    15661 atttgaacct ctcgtgggcg cccgacagcc acgcgctcgc cgccctcgcc tgccgtcagg
    15721 acgaactcga cgcgcgcttg aatgaaggca aagaactcgc cggtcgcccg cgcatcatcg
    15781 aacgcgccga cgggcgcgag cgtctgctcg acgatcaact gatggacgtt cagcccgtct
    15841 ggtcgcccga ctccgcgaag gtcgccaccg ccatcgaaac cgacgtggcg atctacgacg
    15901 cgctcggcca gtcgccgacg ggcgcgcgtc tccccttgcg cgatcctttg ttcaactcgt
    15961 cagccgccta cgatgcggcg aagttaaaac gtgcgactgc gcccgccggg agcggcaaaa
    16021 cagattcgtc aggcggcgac ggctcgacag cagcgacttc aaacagcagc gccacgccga
    16081 tctctttcaa tcccgtcatc agcctcgaat ggccgcagcc cgaaacactg ctcgtccgca
    16141 cggggttcgt gcgctactac cagaacagcc ctgagccgac gcgcagctat ctgcgctggc
    16201 acctcttaca cctcagcccg caggccgccg tgctcagttg aagcgcactc gcagcgtcgg
    16261 cttcacttaa actaaaggag tgaacaaacc cccttgatct accaaagcat ctcagacatc
    16321 tacgccgcca acgagtcggt gcgccgacag gtcaccgccc gcctcgaaac tttaagcgaa
    16381 gcgcaacaaa cttttcgccc ggacgaaggc tgctggtcgg tcgcacagat cgtcgaacat
    16441 cttgcgatca tcgagcagaa catggtgcga ctgatcggca tgctgctcaa caaggccgag
    16501 ggcgcagccg ccgcaccgtc agacgacgcc gccacgccgc gccccttcaa gccgttctcg
    16561 ctggacgctc aggtcgaaac gatcagggat aaaaaactga ccgcgcccga acaggtgcgc
    16621 cccggcggac aggccgcgct cgccgattcg ctcgccagcc tgcaacgcac gcgcgccacc
    16681 gtcgagagct tccgcccgcg cctcgaagcc gcagacctcg aagccgccac ctacccgcac
    16741 ccggcctttg gcgaactaaa catctcgcaa tggctggcct tcatcggcca ccacgaagcg
    16801 cgccacctac gccagatcga gcgtctgctc gatgcgccgg agtttgccaa cgaaaactga
    16861 gactgacggc gaagcgtccg cttgcggcga ctcgcacgcg tctctataat cgcccggcac
    16921 cattcgtaag tttatgccct cttagctcaa cggttagagc agcggactca gttctttgaa
    16981 aggtttaagt ggagaattcg ggattatgaa actccgcgtg gcaattcaga caaaagaagc
    17041 ctgcactttc gggcttcggc ttcgagcgat tcccacgagg tgttcgagca acttctgaga
    17101 tcgataggaa aagacttggt cgcgggatca aggtggggaa agccaacgct gcatggttac
    17161 gtccgtagcc gcatcgttcg ccacaaccat tcttctgttg aatcagccgt tgccttcttt
    17221 gccagccacg ctcttgctgg caggcgtaat tctgatgtct gttgtttgtc gaagcgttct
    17281 tacatcgtcg tgagcaaaat ctaacttgct cgcccgtcaa attcgcccgg cagataacag
    17341 aaccgttctc cataagacct ccaaggttgg gttgcaaacg tatcacactc gatgctccgg
    17401 gttaaccgga aattcgtttc aggtgcgctt gtagaatctg tcaaattcgg ggaagcctaa
    17461 gttgcgaaag cgagacggta atcccgagcc aagccgcgcc gctcatggcg cggaaggtgt
    17521 agagacttga tggcagacgc gtaaagcgaa gagaaagtcc agaccacaaa ctccgtctgc
    17581 cggtacgtca ggcggggcgc ggcgaaagcc gtagcggtaa gcataatccg ttggttgtag
    17641 gttcgaatcc tacagagggc agttttaata cgaaggcggt ttaagggcgg ctcagggggt
    17701 cgcccttaac tgtctccagc gtgcgaaggg cgactccccg gcggaaggat cattcgtgtt
    17761 gagatacgca cgttgtcgcg gcgggcgcgg gcgtctgtct ctgagtgata aacttgtcac
    17821 ctgtcatctg tcacttgtca ctgctctatt gaagggagcg aaaatttgag ccgggaaaat
    17881 tccatcggca tcggcgtgat cgggtcgggg tttgcgcggc ggacgcagat tccgggtttt
    17941 cgcgccgcaa caggcgcgcg ggttgtggcg attgcgagcg cgcggcgtga gcgtgcggaa
    18001 gcggtggcgc gcgagtttgc gattccgttc gtgggcgaca actggcgcga cgttgtagac
    18061 cgcgcggatg tcgatctcgt cagcatcgtg acgccgcccg ccacgcacct tgagatgacg
    18121 ctctacgcgc tctcacgcgg caaggccgtg ctctgcgaaa agccgatggc cttgaacgcg
    18181 gctgaaaccg cgcagatgtg ccgcgcggcg agcgcggcgg gcgtcctcgc ctgcattgac
    18241 cacgaactgc gttttctgga cgggcggcga cgcatgcgcg aactgctacg ggcgggcgag
    18301 atcgggcgcg tcagacatgc gagcgtgact tatcgcgccg attcgcgtgc gaacgcggag
    18361 cgcgcatggg attggtggtc ggatgcgaag atgggcggcg gcgcgctcgg cgcaatcggt
    18421 tcgcatgtcg tagacggcct gcactggttg ctcggcgcag aggtgtcgcg catcttctgc
    18481 caactctcga cgcacatcat tgcgcgcccc gtggccggag agcgcggcga cgagatgcgt
    18541 ccggtgacga cagacgacgc ggccaacctg atcatcaact ttacaggggg cggcctcctc
    18601 gccgagacgg gcgcgagcgc aaacgcctcg ctctctttcg tcgaagcggg agagcctctg
    18661 catcgtctgg agatcttcgg cgagtgcggc gcgctcaggg tggaaggcga cggtgagttg
    18721 tggcgcgcgg agatggaggc gggcgcgtgg cgacgcgtgg aaaccgggag cgcgcacacg
    18781 gtcgctcccg gcatgcagga caacggctgg tcgcgcggct tcacacgctt cgcgcaggag
    18841 atcgtcaacg cgctcctcga agggcgcagc acggtcgaag acgcggccac gttcacggac
    18901 ggtcaccgca tccaactcgt cctcgacgcc gcgcgccgct cgcacgcttc cggcagttgg
    18961 gcgacgccgg accataaagg tttcgaggcg tcctaaagtt ttgacaagac gagatgaaac
    19021 aagctgacgg agcacgaccg cgcaactgga aaaagagagt cgcacccggc gtgctcttcc
    19081 tgctgctgtt cgcgtcgggc ataatctttt tcgccctccc ggtcttcgtc gagaaacatt
    19141 acaaccggac gattcactca ccgccttacc ctgtatcgca atcgacatag gcttctggca
    19201 gacggccacc tgcggcacgg atgcgcgtgc gattgcgcgc agcattcgtt acacggcgaa
    19261 cctcatcggc gcgcgacacg tcggtttggg atcggatttc gacggcgcgg tcgcgcagcc
    19321 tttcgacgcg agcggcctcg cgcaaatcac gaacgcgctc ctcgccgaag gcttcggaga
    19381 agaagagatt aaaatgatca tgggcggaaa cgtgctccgc gtcttcgcgg agcatctgcc
    19441 gcgctgaact tcaaacagct tcttaatttg attcgtaacg ggtgagatga gttgagagtg
    19501 gcgaagcgta agcaaaaaga tgcggggcgc gtcgtcgtgc gcgtcggcgt ggacacgggc
    19561 gggacgttca ccgatttcgt ctatgcgacg cgcgacgggc tggcgggcat gttcaaactc
    19621 gcctccacac ccgccgaccc ttcacaagcg atcacggaag gcttgcggcg tatcgcctcg
    19681 gagtcgggtg tcgccgtcgg cgagttggag atcgtacatg gcacgaccgt cggcacgaac
    19741 gcgctgctgg agagacgcgg cgcgcgtgcc gcgcttgtca cgacgaaagg tttcgcagac
    19801 gtgctcgcca tcggcaggca ggcgcgcccc gcgctctacg atctgaacgc gacgcgaccc
    19861 gaagcgatca tcccgcgcgc cctgcgcttc ggcgtacgcg aacgcatcgc ggcgacgggc
    19921 gaggtgttgg aagcgttgga ggagagcgaa ctggtcgagt tggtcgagaa gttgaagcgt
    19981 gcgcgcgtcg agtcggtcgc cgtctgcctg ctcttttcgt tcgtgcatcc cgaacacgag
    20041 cggaggatcg cgcgtgcgct ctcgaagtta ggcgtgccgc tctccgtctc gcatgaaatt
    20101 ctgccggagt atcgagagtt cgagcgcact tcgaccgtcg ccattaacgc ttacctgcaa
    20161 cctttgatgg gcgcgtatct caagcgtctg cgcgcgccgc gcctgcgcgt gatgcaatct
    20221 tcgggcggga gcatctcggc gcgtgcggcg gcgagcgagc cagtgcgcac tatcttatcg
    20281 gggcctgcgg gcggcgttgt gggcgcgctt cgcgccgcgc tcgccgcagg ctcacccgac
    20341 atcatcacct tcgacatggg cggcacttcc acagacgttg cgctctgcgc gggcgggcag
    20401 atgcgcacga cgaacgaggc gacggtggcc gcgttgcccg tcgccgtgcc cgttttggac
    20461 atccacacgg tcggcgcggg cggcggttca atcgcacgcg tggacgcggg cgggagtttg
    20521 cgcgtgggtc cggagtcggc gggagcggag ccgggtccgg cggcttacgg gcgcggcacg
    20581 atgccgaccg tgaccgacgc caatctcgtg ctcggtcgtt tcggcggcgc acaactgctc
    20641 ggcggcgact tcaagttgga cgcgcgccgc gcgcacgacg cgctggaacg actggcgcgc
    20701 gagttgagtg cggcgagcgg gcgacggacg acggcgcacg aggccgcgct cggcgtggtg
    20761 cgcgtggtga acgccgggat ggaacgcgcg ctgcgcgtcg tgtcggtcga gcgcgggttt
    20821 gattcgcgca cgttcgcgct cgtctcgttc ggcggcgcgg gcggcctgca cgccgtcgcg
    20881 ctcgcagaga gtttgcgcat tccgcgcgtc atcgtgccac ggaggccggg cgcgctctcg
    20941 gcgttcggcg cgctcgcttc ggatgtcgtg ctggatgcga gccgcaccgt gatgctcgat
    21001 gcggcgggcg gcaggctctc cgaactcgaa cgcgcgtttc aagcgatgga acgcgcggca
    21061 cgcaccgtgc tcagccgcga aggcttcaca ggtgaacggc agcagagaca cgcgcgcagc
    21121 atctctgcgc gttacaaggg acaatcgttc gagttggaga tcaactatag gtcgcggcag
    21181 acgctcgccg cagcgtttca tcgcgcacac ctcgcgcgct acggttacgc gctggcggag
    21241 aacgcggtcg agatcgtgag cgcgcgtttg cgctcgcgcg ggctggtgga aaaattgaag
    21301 cgcgaacgcg tcgctcaaaa cacgaccggc tccaaagtcg cgccgcagtc aacggcttcc
    21361 gtctacttca agaccggagc gtcgcgcgtg cccgtctacg cgcgggaaga actcaaggcg
    21421 ggcgcgcgtc tgcctgtgcc ctgtatcgtc accgaatatt cttcgacgac gctcgtgccc
    21481 gaaagtgcgc gcgccgcgcg aattgatgaa cagggcaacc tcgtcatcga actatgaaac
    21541 gcacgcccac tgtaattgaa ctatgaaacg cacgacttac gatcccgtca cgctggagat
    21601 ttatcgcgcg ctctacactt cggtcgcgga ggagatgggc atcgccctca ggcgcacggc
    21661 gcattcgccg aacatcaagg agcggcgcga ctattcgtgc gctgtcttcg acgcgggcgg
    21721 gcgcgtcgtc gcgcagggcg accacatgcc ggtgcatctc ggcagcatgc cgatggccgt
    21781 cgccgccgcg cttcgtgaaa ccgggatcgc acccggcgat gtcgtcgcac tcaacgaccc
    21841 gttcgcgggc ggcacacacc tgcccgatgt cacgctcgtc gcgggcgtct ttgacgtgaa
    21901 cggaagcgcg aagggggaaa ggggagagag aaagacacga agccgcacgg cgacggacgc
    21961 gtcgaagaac agcgcgatag agaataatgc gtcggcggac ggcgcgatag aggatgacac
    22021 gctagagaat ggcgatgcgg ggcgcagcgg tcttgaccct tcccccttcg ccctttcccc
    22081 ttccccgatt ttctacgtcg ccaatcgcgc gcaccacgcg gacatcggcg gcggttcgcc
    22141 cggttcgatg ggcatggcga cggacgtgta cggcgagggc ttgcgcatcc cgcccgttca
    22201 cctcatgcgc ggcggcagga tgaacgaaga cgtactcaga ctgttgctgg cgaacgtgcg
    22261 cggggctgac gaacggcgcg cggatttcgc ggcgcagacc ggatcgctca agaccggcgc
    22321 gtcgcgcctg cttgagatcg tcgcacgcag gggcgtgagc gaagccgcga gctatgcgtc
    22381 gcacctcatc agttactcgg cgcgcatgat gcgcgcacgc atcgccctga ttccagatgg
    22441 agtctacgcg gcggaagacg cgctcgacga tgacggcacg agcgacgaac cgtgccgcgt
    22501 gcgcgtgcgc gtggagatta aaggcgcgcg ggcgcggatt gattttacgg ggagcgcgcc
    22561 gcaggtggct ggtcctgtca acgctgtcga agcgatcacg gtgtcggcgg tcgcttacgt
    22621 ctttcgctgc ctgctcggcg cagaggaagt cccggcgagc gcggggctga tggagccgtt
    22681 agaagtcgtc gcgccgcccg gcaccatcgt caacgcgctt gcgcccgcac ccgtcgcggg
    22741 cggcaacgtc gaaacttccc aacgcatcgt tgacgtgctc ttcaaagcat tggcgcaagc
    22801 cttgcccgaa ctggtgcccg cggccagcca gggcacgatg aacaatctca ccatcggcgg
    22861 cgtggaccag aggacggggc gcgagttcgc ttactacgag actatcgcgg gcggcatggg
    22921 cgcgcgtccc gcgcaagcgg ggctgagcgc ggtgcacacg cacatgacga acagcctcaa
    22981 cacgcccgtc gaagcgctcg aatacgcata cccgctgcgc gtgcgcgagt atgcgatgcg
    23041 cgacgcttcc ggcgggcggg gcaagcatcg cggcggctgc ggcgtcgtgc gcgagatcga
    23101 gacgctcgtg cccgcgcgca tggcactgct cgcagaccgc agacgccgcg cgccttacgg
    23161 gctggcgggc ggcgcagagg ggcgcgccgg acacgactct gtgatcggcg gcgacgggcg
    23221 caagcgacgc atcaagtcga aaggctctca cgaactgaaa gcgggcgacc gcatccgcat
    23281 tgagacgccc ggcggcggag gacacgggaa gaaacgatga acgcagaacg atgaatgatg
    23341 aatgacaagc aggattcagg attcagaagt cagaataaaa cctgcgcggg tcttcatgct
    23401 gattcctgaa ttctgttttc tgaattccag ttcatcgttc atccttcatc cttcgccgtt
    23461 tccccctcct tctttccttc gtcggttgtt caatttagaa tgtgctttca caaagacgtt
    23521 tggaggggga acggttatgc gcgtcaccat cggacagatc aacacgacga acggcgacta
    23581 cgagggcaac gtcgcgcgga ttttacacgc catcgagagg gcgcgggcgg aggggagcga
    23641 tctcgtcgtg ctgccggaag tcgccgtgca gggctacatg tcgttcgatt ggtttttaga
    23701 ccgcgacgtg gtcgcacacg cgatggagcc gctcgatgag atcatcgcag cgagcgcggg
    23761 gctgacggtc attgtcggga cggtgcgccc gaccgggcag accggaggcc gacgcctcta
    23821 caactcggcg gcggtcatct ccgacggcaa actcttaggc ttcgccgaca agactctgct
    23881 tcccgaatac gacgtgttcg acgacccgcg ctacttcgaa ccggcaaagg aacgccgcct
    23941 gttcgacatc gaaggcatgc gggtcggcat cgccgtctgc gaggacatgt ggaacgacaa
    24001 gacctactgg cgcgagcggc tctatgccaa cgacccgacc gacgacctga tcgcgctcgg
    24061 cgcgcaactg atcgtctcca tcaacgcgtc gccgttcaac aagagcaaga tgggtcaacg
    24121 ctgttcgatg gtttcgcatc gcgcgaaagc ggcgggcgtc cctatcgtct tcgtcaacct
    24181 cgtcggcgca aacgacggcg tcatcttcga cggcgcatca atcgtcacag accggaaggg
    24241 acagatcatt ctgcaagcgc cgcccttcga agagttctac ggctcggtcg atctcgattg
    24301 cgaccagtgc gacacctact gtctgcccgg cgacgacatt caaaccgtac acgacgcgct
    24361 cgtcctcggc atacgcgact acgcgcgcaa gaaccgtttc gagcgcgccg tgctcggtct
    24421 ttccggcggc attgattccg cgctcgtcgc cgcgctcgcg tgcgaggcgt tgggcgcggc
    24481 gaacgtcttg tcggtgatga tgccttcgcc cttttcgtcg cgcgggagcg tggatgattc
    24541 cgtcgcgctc ggcttaaatc tggggatgcc caccatcgag catccgatca gcgcggctta
    24601 tgaagtgctc gtcaaacagt tgaaactgga gaaccccggc acgggtcagg aagaactcgc
    24661 gcggcagaac acgcaatcgc gcctgcgcgg caacatcctg atggcgattt caaatgccga
    24721 aggtcgcctg ctgctctcga cgggcaacaa gtcggaactc gcgctcggct actgcacgct
    24781 ctacggcgac acaaacggcg gcctcgccgt cctcggcgac gtgctcaaga cggaggtgtg
    24841 ggcgctctcg cgttatctca accgcgagcg cgagatcatc ccgcgcgcca tcatcgaaaa
    24901 acgcccctcc gccgaactcg cccccggcca gtttgacgat caatcgctgc cgccctacga
    24961 caaactcgac ccgatcctca aactctattt cgaactcaaa gccacgcccg aagagatcgt
    25021 cgccaaagga cacgaccagc aactcgtctt cgacgtgctg aaccgcgtcg aagccccggc
    25081 caacgaattc aagcgacgac aactcccgcc cacgctcatc atctcgcgtc acgccatcgg
    25141 catcgggcgt cgccgccccg tcacgcatcg ctaccggcgg caggtcttga aggccgtcga
    25201 gcgcgcggct gaagggcagg gataaaagag aagccttcaa cgcagctagc atgaagcggg
    25261 cgcagagaga gagagagttg atacaacgct cttctctctg cgcccgcttc gacatcttct
    25321 gtacctgcga tggtgaacgc ctcgattgct ggtcgctctc aatcgcaaac gccattcaag
    25381 agattgaaac taaggtgggg atgagttcgc ctgcggggtc ggcgtggccg atggccgcgt
    25441 cgagttcggc ttcgaggtct gcgggggaga cgggcgcgag tttgcggaag tcgaagagtt
    25501 tcgggtcg
//
LOCUS       DASWNS010000549         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_21283, whole genome shotgun sequence.
ACCESSION   DASWNS010000549 DASWNS010000000
VERSION     DASWNS010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21283"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VGB76_18960"
     CDS             <1..662
                     /locus_tag="VGB76_18960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0101014.1"
                     /translation="FPTPRSVAAGLGEEVRTGRLADDLVASLFRVAAGYTLAVILSVP
                     VGLALGHRPAVRAALLPAINFFRSLSPLAWIPFAILWFGIGDLPVVFLIFMATFFPLV
                     LATLAAVANVPSVYFQVAHEYGFRGAELLTRITLPAIMPQVITALRVTGGMAWLVLVA
                     AEMIAGRDGLGFAIWDARNGLRLDLLVCGMFVIGLTGVLLDWLLALLAKLPSVRWGYE
                     H"
     gene            652..1440
                     /locus_tag="VGB76_18965"
     CDS             652..1440
                     /locus_tag="VGB76_18965"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0101015.1"
                     /translation="MNTNTLVVRDVSHSFGGALVLDRITLEVASGEVVALIGPSGCGK
                     TTLLRLLTGFYQPERGAVERRGGTRMIYQQDGLLPWLTVAGNISLGLRHVADAAEREE
                     HLARLLKLIRLQGFARHYPHQLSGGMRQRVELARALAGDINVLLMDEPFSSLDYQTRS
                     RMRSELTRVLALRPVAVVLVTHDIEEAVLLADRVVVFSERPARIIRELRLDAERPRDV
                     TDPTLRAAMKSILEHLALPHDFDGDAPPDEPEALGMPEASAWPD"
     gene            1498..1665
                     /locus_tag="VGB76_18970"
     CDS             1498..1665
                     /locus_tag="VGB76_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101016.1"
                     /translation="MTRTRIFIIALGLLVLIGGLGLLRFASLKRRNATDGGGSDGIAR
                     QQLKVGFLPVT"
     gene            1720..2523
                     /locus_tag="VGB76_18975"
                     /pseudo
     CDS             1720..2523
                     /locus_tag="VGB76_18975"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(2635..2883)
                     /locus_tag="VGB76_18980"
     CDS             complement(2635..2883)
                     /locus_tag="VGB76_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101017.1"
                     /translation="MRFEELMKVVGGEAEREELTRLGREYADAHRLTHERAGPQAAAQ
                     REAALGALMDKVQFHFRAIRHPLPTRQQLEEMLSEHFE"
BASE COUNT          508 a         1067 c          952 g          595 t
ORIGIN      
        1 cctttcctac gcctcgcagc gtcgcggcgg gactcggcga agaggtgcgg acgggcaggc
       61 tggccgacga cctcgtggcg tcgctctttc gcgtcgccgc aggctacacg ctcgcggtaa
      121 tcttgagcgt ccccgtcggg ctggctctgg ggcatcgtcc ggcggtgcgc gccgcgctcc
      181 tgcccgccat caacttcttt cgcagcctct cgccgctcgc gtggatacct ttcgccatcc
      241 tctggttcgg catcggcgac ctgcccgtcg tgtttctgat cttcatggcg accttcttcc
      301 cgctcgtgct cgccacgctg gcggcagtcg ccaacgtgcc gtcggtctac tttcaggtcg
      361 cgcacgagta cggctttcgc ggcgcggaac tgctgacgcg gatcacgctc ccggccatca
      421 tgccgcaggt catcacggct ctgcgtgtga cgggcggaat ggcgtggctc gtgctggtgg
      481 cggcggagat gatcgccggg cgcgacggcc tcgggttcgc catctgggac gcacgcaacg
      541 gcctgcgtct cgacctgctc gtctgcggga tgttcgtcat cggcctgacg ggcgtgctgc
      601 tcgactggct cctggctctg cttgcgaaac tgcccagcgt gcggtggggt tatgaacact
      661 aacactctgg tcgtccgcga cgtgtcgcac tctttcggcg gtgcgctcgt cctcgaccgc
      721 atcacgctgg aagtcgcttc aggcgaagtg gtcgcgctca taggtccttc cggttgcggc
      781 aagacgacgt tgctgcgcct gctcacgggc ttctaccaac cggagcgcgg cgcggtcgaa
      841 cggcgtggcg gcacgcgcat gatctaccag caggacggac tcctgccctg gctcaccgtc
      901 gcgggcaaca tctcgctcgg cctgcgacac gtggccgacg cggcggagcg cgaagagcat
      961 ctcgcacgcc tcctgaaact gatccgcttg caggggttcg cgcgccacta cccgcaccag
     1021 ctttcgggcg gcatgcgcca gcgcgtcgaa ctcgcacgcg cacttgcggg cgacatcaac
     1081 gtcctgctca tggacgaacc tttctcttcg ctcgactacc agacgcgctc acgcatgcgc
     1141 agcgaactga cgcgcgtgct cgcgctcaga cccgtcgccg tcgtcctcgt cacgcacgat
     1201 atagaggaag cagtgctgtt ggccgaccgc gtcgtcgtgt tctcggagcg tccggcgcgc
     1261 atcatccgtg aactgcgtct ggacgccgaa cgcccgcgcg atgtgaccga cccgacgttg
     1321 cgcgccgcga tgaaaagcat cctcgaacac ctcgcgctgc cgcacgactt cgatggcgac
     1381 gcgccgccgg acgaaccgga agctctcggc atgcccgagg cgtccgcgtg gccggattga
     1441 accgtagcat tgtctctttg aacttaaact cgaaggccgt accggagaat cggatagatg
     1501 acacgcacca gaattttcat tatcgcgctc gggcttttag tcttgatcgg cgggcttggc
     1561 ctgctgcgct tcgcctcgct caagcggcgc aacgcgaccg atggcggcgg cagcgacggg
     1621 atcgcgcgcc agcagttaaa agtgggcttc ctgcccgtta cttgacacct cacgtgcccc
     1681 gtcacggact tcgcgtcacg gacgagcacg acgactcggt tcgagtcgca aagattcaca
     1741 gactttccca cggttaccga gacggtgaaa tccggtcgcc tcgatgcgac cttcatggtc
     1801 gtgccgcttg cgatgaaact gcgcgagcag ggactgccga tcaagatcgt ctatctaggc
     1861 caccgcgacg gctcgaccgt catcgtccgc aaggacagcc ccgcacgcaa cctctcagac
     1921 ctgcgcggca aaattttcgc catccctacg cggttcagca accagttcat cgtcatccgc
     1981 aagctgatgc aggacagcgg gatcgcgccc gaagagatca acttcgtcga gttgccgccg
     2041 ccggatatgc ccggcgcgct cgcagccaaa gcgattgacg cttacttcgt cggcgagccg
     2101 cacgcggcga aggccgaact cgacggcacg gggcgcgtcc tctatcacgt caaagacatc
     2161 tggccgcact tcatctcctg ctgtctcgtc gttacggaga aactgatccg cgaacgcccg
     2221 gaggtggtgc gggatttggt gcgagggatc gcggagtcgg gcgagtgggc ggagacgcac
     2281 aggccggagg cggcgcgcgt cgccgcgcct tacttccggc agaacgaaga ggtcatcaag
     2341 tacgtgcttg agcagccgcc cgaccgcgtg agctatcgca tgctgtcgcc cacggacgaa
     2401 gacctccaaa agattcacga cctcgcgctc agtctcggcc tcatcactaa gcccgtcctg
     2461 atgagcgacc tcatcgaccg cagttttatc cccgccgaca tcaaaccggc tgacatcaaa
     2521 tgagttacgc ttgagaagtg gaaccagtcg ccggtttcac ttctcaagcg cgtcttgcac
     2581 acgggcgcgc cgccggacga aagttcggcg gcgcgcccgt ctctcttcaa cgcatcactc
     2641 gaagtgctcg ctcagcatct cttcaagctg ctggcgggtc ggcaatgggt ggcgtatcgc
     2701 gcggaagtga aactggactt tatccattaa cgctccgagc gcggcctctc gttgcgctgc
     2761 ggcctgcggc ccggcgcgct cgtgcgtgag acgatgcgcg tctgcatact cgcgcccgag
     2821 gcgcgtcaac tcttcccgct cggcctcgcc gccgaccact ttcatcaact cttcaaatct
     2881 catcctgctg cctctcgcgc caacctccgg cgcatatgcg agaggggctt aaagcgtggc
     2941 gaacgccttc aagcccctcg tccttcaatc agtcgcccgc gcaaggcagt gtgagccgcc
     3001 cgcgcgagtt atgcacgctt gcttccgcga cgggtcttcg cacccgcctt cgccttgctt
     3061 gccttcgcgc ccgctggcgc agagggttgt gcggttgagc gacgagcgcc gcgaccgttc
     3121 ct
//
LOCUS       DASWNS010000550        18666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_331343, whole genome shotgun sequence.
ACCESSION   DASWNS010000550 DASWNS010000000
VERSION     DASWNS010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18666
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331343"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VGB76_18985"
     CDS             <1..705
                     /locus_tag="VGB76_18985"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101018.1"
                     /translation="TREFYFLEMNTRLQVEHPVTELVTGIDLVREQIMVAAGQRLSFT
                     QAQVEWRGHAIECRVYAEDPDNNFLPSPGRITHLRVPAGPNVRDDGGVYEGAEVSIYY
                     DPMISKLAVWGRTRPEAIERMRRALAEYTVGGIKTTLPFFREIVRDAEFIAGQLDTGF
                     IERFNGRRAAALTAAQSEEVPGKEIARRDMAVIAAALAYESGAKTASQQQSAAGDTQS
                     SRWKIAGRNALHQSRS"
     gene            complement(873..1478)
                     /locus_tag="VGB76_18990"
     CDS             complement(873..1478)
                     /locus_tag="VGB76_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101019.1"
                     /translation="MLKNESSTQRGRKLLFVSLVACLLLAAVLLSPLGSRRLVAARTE
                     AAAATSPAPFQAVRSPWTAVGSTGVIDEASANFHAFGSADIGFRPGSPGTLITARYNV
                     TNTFDNNPDPNFPGWATLEMGSNAPINTMIEARLFRIKTCDFTPELLCIARNRSNDFP
                     CARCSIPFPIDFTSNLYYVEVVLDRTAAAGSFPRMFTLRLF"
     gene            1690..2340
                     /locus_tag="VGB76_18995"
     CDS             1690..2340
                     /locus_tag="VGB76_18995"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY0101020.1"
                     /translation="MSTDLRADAPVRTADAETGVDGEEVSESTSIQLGRQESTARRAR
                     KFTMKLIAEITGTKQNLEIRRDGARVWAEIDGRLYELEAHEPEAGVYLLMHGGGVYEC
                     RVETGVGTARGSSEVSVRDSVYEVALIDPKRLRGARGAGAEASGRAQVAASMPGKVVR
                     VLVAEGAQVEAGDGLVVVEAMKMQNELKSPKSGTVVEVRAEAGATVNAGDVLVVVE"
     gene            2359..3120
                     /locus_tag="VGB76_19000"
     CDS             2359..3120
                     /locus_tag="VGB76_19000"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY0101021.1"
                     /translation="MREAEEAEGIAPEDLRVLLWDIDGTLLRSLRAGAFKDYTVPVLE
                     SLFGTAGSIHEMRVSGMTDLQIICEALRDEGITTEQVRARVGEIRTRFMAEMERVATR
                     AAQDESPLFQLLTGVREILERVAAHPRYRSSLLTGNIEPAAHLKLQLVGLEGFFQELP
                     GAFGDDSHDRRDLPALAAARINARLKLSLRPAQFIVIGDTPNDIACARFFGARSVAVC
                     TGRFDTRESLAAHNPDAILPDLSDTDSVLRTFDAL"
     gene            complement(3382..5502)
                     /gene="mutL"
                     /locus_tag="VGB76_19005"
     CDS             complement(3382..5502)
                     /gene="mutL"
                     /locus_tag="VGB76_19005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY0101022.1"
                     /translation="MSKIRVLSDHLANQIAAGEVVERPASVAKELVENSIDAGARRIE
                     LDVEAGGRRLLRVSDDGEGMTQDDALLAFERHATSKISTADDLNNISTLGFRGEALAS
                     IASVARIELITQTEGEAAGTRIVVEGGRMRDVKPAARPRGTTITVRDLFFNIPARRKF
                     LRSEATESFHLTSLVTHYALAHPEIAFVLTNNGRESLRAGVAANLRERAYQIFGADFL
                     NSLLEVNGGHERVARVSGYVSAPRERRTSRDAQYLFVNGRYVRDRLVSRALSEGYRTV
                     LPHGVYPAALLFLEVPLEEVDVNVHPAKTEVRFRRAPAVAEAVREAVRGALAAGGYVR
                     RADEPDERRVETQSPGEFANFDRAGVEASGGDAAWRAKSEAQLPQQEPMEFGFTPPTD
                     EELLLSSQEFAERAQSLPPPARAALDTMERNAGTATPEREEYIDVRGANDPDASMLAT
                     DERARGREAQGDEARLRADNELRTEAGARDLNSSAPAPSQGSASSLPPLNSIAGAMYE
                     VEVEAISNNIRPLYQLEESYIIAIDAEGVLLIDQHAAHERVLFDKYRKLEQTRAAESQ
                     ALLMPETFDLTPAQAAAFDTVAEELETYGFSLMRLSGRTVAVKAVPADLPATEARNML
                     AEVLETVEAERRGTLRETLRERVAASLACHAAIKVNMPLAIEKMRWLIDRLLTTSSPT
                     TCPHGRPAILRLTTRDIERGFHRP"
     gene            complement(5607..7316)
                     /gene="bshC"
                     /locus_tag="VGB76_19010"
     CDS             complement(5607..7316)
                     /gene="bshC"
                     /locus_tag="VGB76_19010"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEY0101023.1"
                     /translation="MSTELTPTAETEPACFSTPQEAGLRIETLPFARIPQQSRLFLDY
                     LADPTALRRYYPNAVRFHHELAARAPEVLAAHKTDRNVLCDALEEMNTRWGAGAETER
                     NIARLRSPASIAVVSGQQVGLFTGPLYTIYKALSAVKLAGCLTQRGTEAVPVFWMATE
                     DHDWEEVQAAEFTACDGRLASTNLPDALHREGAPVGGVRLDETVSDAVQRLLDLLPTT
                     EFLPDLESLLRDAYAPGRTHGEAFARFLTALVSRHGLVLLDPLDPSLKRLAAPLYSEA
                     ARRAPEIAAAVEARSRELERENYHAQVHTSPDAFPLFIHLEGSRRAITRNSEGLYQAK
                     GATGGKTYTAEELAELAASEPERFSPNVTLRAVVQDYLLPTIAYFGGAAEIAYFAQTG
                     EVYRLLDRPATPILHRASLTFAERRTGRTLERYNLQLADFFAGLDAVIARVVEEHLGA
                     EQAQAFDRAEASIHSALGELGDNLRRFDPTLADALTHGTQKIEHQLGGLRTRFHRAQM
                     ARDRAAHRQLERAYTALYPEKTLQERHLNITSLLARHGRYCLDWIYDAIDLGSVEHQI
                     VYL"
     gene            complement(7469..8434)
                     /locus_tag="VGB76_19015"
     CDS             complement(7469..8434)
                     /locus_tag="VGB76_19015"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY0101024.1"
                     /translation="MKSHDTIHLRDSRDGGLTAKRLRGILLPCTTPFDSDGRPDTRAL
                     ASNIERWNETGVSGYVMLGSTGERVHLDEGECREVVEAARASVPAHLAFVVGVGQHDT
                     RRSIAEAERACAAGADALLLITPHFYRGQMTPAALTDYYKAVADASDVPVVLYNIPQN
                     TGIALSPETIARLSEHEKIIGIKDSSGDMVNFMEMLSLQTDNFAVLTGHASVLYAALA
                     AGAQGAILAVGCVAPGVAVKIARLFEEGRHEEAREVQRKLIPLARAVTTRYGIGGLKY
                     ALDLAGYAGGEVRAPLRLPDETARREIERTLAECRPYVKAVTSDE"
     gene            complement(8561..11767)
                     /locus_tag="VGB76_19020"
     CDS             complement(8561..11767)
                     /locus_tag="VGB76_19020"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0101025.1"
                     /translation="MSTSPNENAFAATSPRLPYQKLEAHCVEQHLYPLYRVLEDEYVH
                     QYGQLPETYLQAKAGLDLAVKEQIKGEIKHALEHAEKTEHREQHRRGLPRQASAPQVS
                     EAELEQRATEEIEKSYDQAGGMRPTRYVVLLNEKFTAELYRHIHDLPEKRTALCFSGG
                     GIRSASFGLGIVQGLARYGMLGHFDYLSTVSGGGYLGSWLSAWIHRSRLPNVIGALRN
                     EPEKNKLRPEPEPIRHLRSYSNYLNPKLGFLSADTWTLVAIVVRNLVLNWLLLIPIIL
                     AALMLPRIVAGIVQLNFKTGLPVHWAFWLGTIFGLIAMVYMGVARPSANPPRKINSQR
                     LNQQGWFLTLCLLPLSLSAICLTSGWAWRRPGEQGDWLGEHWGEFVQYGVALHLAAYI
                     LYTLILGCKNFKFWRERWARDWPRLAWEPLAVILIGAFWGTVTWWAATIAFPTPARPG
                     VLPSPVTELYACFAAPLFMCLFLFSVILFVGLTSNFTDDDDREWWARFGAWVLIVSLL
                     WSVVSALVIFAPVGLLYVFMQSVVLQAILTALGGASGLFTIFGGKSEKTSSHAEQEGK
                     SGGLAAYLMPLAAPIFALTLLIVFSLGTSLLIRSLGVRLAGGRLVPASFNWHDAERLS
                     YDSYGLLNIIDFAPLNLLLLLTFVLVTISLVMAAFVNVNKFSLHSMYRNRLIRAYMGA
                     SRPRAERRPNPFTGFDTYDNIAMHELSHFAGALEDKKGREDKEARKTKLLHVVNICLN
                     LTSGHNLAWQQRKAETFTVTPLHCGSFHLGYRRTREYGGKGAPPGDGISLGTAVTISG
                     AAASPNMGYHSSPALAFLMTLFNARLGWWLGNPGPAGNGRFFHSYNRSGPRFALRPIL
                     TEALGHSDNINPYVYLSDGGHFENLGIYEMVLRRAHLIVVSDGSQDPQSRFEDLAGAV
                     RKIRIDLGIPIDFIGGMPIYGKDDAAHKGQTKYCAVGLIRYSCADKDLSGAPAKDGVI
                     IYLKPAITGGEPVDVLNYATASASFPHESTADQNYTEQQLESYRALGSHVIQTICDLP
                     LRDLPLMADDADNLIKRAYYQVPPAMQPTDLKKRFNIS"
     gene            12151..12282
                     /locus_tag="VGB76_19025"
     CDS             12151..12282
                     /locus_tag="VGB76_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101026.1"
                     /translation="MSRLFLQTSGGCDRDARRGLQRRTAGTREMSDGLPSGAGACEY"
     gene            12288..13295
                     /locus_tag="VGB76_19030"
     CDS             12288..13295
                     /locus_tag="VGB76_19030"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0101027.1"
                     /translation="MSGARDERLDLLIIGAGPAGLSAAAAAAREGLSYLVIEKGLIAD
                     TIYRYPVGRTVFSTPDELELREDSLHPCRDKPTREELLSHYVRFALDERLRVNTEEGA
                     EAIERDGQSGLFTVRTTRADYTASRVLVATGAMAYPRRLNVAGEDLPKVSHRFVEPYP
                     YVRKEVLVVGGGNSAAEAALFLCEEGARTSLAIWRADWEQRDPKMNAIKHWVRTPLER
                     ELAEGRLQLLFFTRLEEITEREVRLQMEDGATRTVPNDAVFILIGADADHTLLRRAGV
                     EFSNAGLAEAPVYDPETYETNVPGLYVAGHFTHARHIKQAIAVPRLIVPRIAQSLRTM
                     V"
     gene            13620..13985
                     /locus_tag="VGB76_19035"
     CDS             13620..13985
                     /locus_tag="VGB76_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101028.1"
                     /translation="MKRINSVTYEVLPGEKLSIKVTPTNFGPSAPAVEADLDGTTLTN
                     TGTKSKPVYKFTVNKQDGETNLVTMEFVFQFDSPDESFYAVAISGQNDVGCPCGFTVA
                     KPDEDHGAEIAFDVVEEEE"
     gene            14047..16053
                     /locus_tag="VGB76_19040"
     CDS             14047..16053
                     /locus_tag="VGB76_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101029.1"
                     /translation="MKRYASLKLLLALLLCLSPTGSAQTALNCDQLREKIAQLEKMDL
                     KAMSPSIQQIYRESLLKLHTQFGQCLQRDISAMTDMQKAVAGTDAAPDVEDKLRGLQK
                     ERAETDAKLALLRTASNLSTPQSTADAPPAPTETASSDEPAREQPDADDAVNTDPASP
                     AGATTPSPVAVQTTGAAITCAPPLTYADPPSILSDIAGKDAADVVRNNDPDRAIRSLS
                     QMILYTLSDAASPASSEMVRTLEAYQFLGETARTDKQLGASAKSDGAVSAIEKPGFAR
                     LLGFAIEHGGINKKNDGTNLTLSTSLYSLYTFNRGDTAETYDRAGVLNRVGVAATFNI
                     DNTDDELANARRNNLSEWSVKARLFGDRSTRSASFQKFFDAEIRPLIRARLASLGGAT
                     NQLSQRLPVFNQIARAGRRCLPTQVRTRMDAPDYKSATPEAQQKIISDLILGHLKANV
                     LDRVANGTLKLDAPDVAFIEVEFLPGLKTAIGNLLMGRDLLEKRIEDLKKSPLGTLAY
                     TNHRQPLASDYSEAKFLFEQDKSFFRPLKLMGNLGVSFYHSPDRALNQQKLRDISAAI
                     SFEGTTASPFTEAENLSKITYSFVGRYERLFENRRMANRKPDLATAQFVMEIPFIRGL
                     SLPFSLTYANATEEERKKNVRFNFGMRLDTDKLFDLLRAPSASR"
     gene            16312..16956
                     /locus_tag="VGB76_19045"
     CDS             16312..16956
                     /locus_tag="VGB76_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101030.1"
                     /translation="MRCLVKRLSAFSVLFLLLGAVESRAQVMTIKSGGIVHSGNPMVS
                     PVTPHGFYFNGDGITAGSSDERGASVGMDCNPCAPGQTVRLNVTISRFTVPAHGSATL
                     NDLFYTNVWFRGSEFKLSTTTVTIPDGDAPEGLKARFTMTGNLIGYTFPFTSPLINRE
                     VEGQGAVRIYFTKSHLPDRPGYIFQRAIYNFEARPARSRKEASLPSRPSPLAQQ"
     gene            complement(17097..18530)
                     /locus_tag="VGB76_19050"
     CDS             complement(17097..18530)
                     /locus_tag="VGB76_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEY0101031.1"
                     /translation="MPNTPSVSYSSTLRVKLSSRTGTLGELTTALGGAGADIGAIDIV
                     NASRDSITRDITFTASSGEHVKEIVEAARDIDGVEVINVSDRTFLLHLGGKIEVVSKT
                     PLKTRNDLSMAYTPGVARVCEAIHRDPEKAFTLTIKRNTVAVVTDGTAVLGLGDIGPA
                     AAMPVMEGKAMLFKEFAGVDAFPICLSTKDPDEIVRTIKLISTAFGGVNLEDISAPRC
                     FEIEERLKEELDIPVFHDDQHGTAVVVLAALINAAKLIGKPMSELKVIVNGTGAAGVA
                     CTKIIMAAGVSNIIGCDQHGILYRGRREHMNWVKEWYAQNTNPEGMRGTVHDAIVGAD
                     VFMGLSAPGVISREDVKRMTAKPIIFAMANPTPEIMPEEVADIVAVMATGRSDYPNQI
                     NNVLCFPGIFKGALACRATRINEEMKLAAANAIAGLISDEELHPEYIVPSVFDKRIAE
                     AVSREVEEAAYATGVARRERAQVEPSL"
BASE COUNT         3561 a         5664 c         5983 g         3458 t
ORIGIN      
        1 acgcgtgagt tctacttcct tgagatgaac acgcgcctac aagtcgagca tccggtgacg
       61 gaactcgtca cgggcattga tctcgtgcgg gagcagatca tggtcgctgc agggcagagg
      121 ctctccttca cgcaggcgca agtcgagtgg cgcggccacg ccatcgagtg ccgcgtctat
      181 gcggaagacc ccgataacaa cttcctgccc tcccccggtc gcatcacaca cctgcgcgtc
      241 cccgccggac caaacgtgcg cgacgacggc ggcgtctatg agggagcgga agtttccatc
      301 tactacgatc cgatgatctc gaagctcgcc gtctggggac gcacgcgccc cgaagccatc
      361 gagcgcatgc ggcgcgcgct cgccgaatac accgtcggcg gcatcaagac gacgcttccc
      421 ttcttccgcg agatcgtccg cgatgcggag ttcatcgcag gacaactcga cacaggcttt
      481 atcgaacgct tcaacgggcg acgcgccgca gccctgacag ccgcgcagag cgaagaggtg
      541 cccggcaaag agatcgcgcg ccgcgatatg gcggttatcg ccgccgcgct cgcttatgaa
      601 agcggcgcga aaacagcctc gcagcagcag tcggctgcgg gcgacacaca atcgagccgt
      661 tggaaaatcg ccggacgaaa cgcgctccat caatcacgca gttgagcaga actcttcgct
      721 tgagggtgga ggctcttgca tcagggggcg gcgtcggtgt cagcggttcg gcggcttgaa
      781 gcgttaatcg ccggcaggtt gatccatcag gcatcaacca taccggcgat tagactgcgg
      841 cgcagagaga gagagcgcgc gcacgtcaag tatcagaaga ggcgcagcgt gaacatgcgc
      901 gggaaagacc cggcggcagc ggtgcggtct aacactactt cgacgtagta taaattagag
      961 gtgaagtcta tcgggaacgg gatgctacat ctcgcgcagg ggaaatcgtt gctcctgttt
     1021 cttgcgatgc agagtaactc tggtgtgaag tcgcaggtct tgatgcggaa caggcgggct
     1081 tcgatcatgg tattgatggg cgcgttcgaa cccatttcca gcgtagccca gccggggaag
     1141 ttcgggtcgg ggttgttgtc gaaggtgttg gtcacgttgt agcgggcggt gatgagtgtg
     1201 ccgggtgagc cgggtctgaa acctatgtcg gcggagccga aggcgtggaa gttggcggaa
     1261 gcctcgtcaa tcacgccggt tgaaccaacc gccgtccagg gcgacctgac ggcctgaaaa
     1321 ggcgcaggcg aagtggccgc agcagcttcc gtgcgcgcgg cgacaagccg ccgactgccg
     1381 agcggcgaca acaataccgc cgcgagcagc aggcaggcga cgagcgagac gaacaataac
     1441 tttcttccgc gttgagttga ggattcgttt tttaacatga gggtgctcct tcggttgagt
     1501 gtcggttctt ccgtcaggtt gcgccgtcaa gatgagtctc cggcagcgtg agaacgggcg
     1561 aaagaatcgt gatgagttag ctaaagtgga cgaggttgtc ttggaggtta attaggaagc
     1621 gcactgtgag gtgcacgtta aagtgagaac cctccaagtt cgcaaggctt atacaacgga
     1681 cagataactg tgtcaacaga ccttcgcgcc gacgcgcccg tgcgaacagc agacgcggag
     1741 acgggtgtgg acggcgaaga agtttccgaa tcaactagca ttcaacttgg caggcaggaa
     1801 tcaacggcgc ggcgcgctcg taaatttacg atgaaactca tagcagaaat aaccggcaca
     1861 aagcagaacc tcgaaatccg gcgcgatggc gcgcgcgtgt gggcggagat tgacggacgc
     1921 ctctacgaac tcgaagcgca tgaaccggag gcgggcgtct atcttttaat gcacggcggc
     1981 ggggtgtatg aatgtcgcgt ggagacgggc gtggggacgg cgcgcggctc aagcgaagtg
     2041 agcgtgcgcg actccgtcta tgaagtggct ttgatcgatc cgaagcgtct gcgcggtgcg
     2101 cgcggggcgg gggcggaggc gagcgggcgg gcgcaggtgg cggcttcgat gccgggcaag
     2161 gtggtgcgcg tgctcgtcgc ggagggcgcg caggtggaag cgggcgacgg tctggttgtc
     2221 gtcgaagcga tgaagatgca gaacgagttg aagtcgccga agtccgggac ggtggttgaa
     2281 gtgcgcgctg aggcgggcgc gacggttaac gcgggcgacg tgctggtcgt cgttgaataa
     2341 gggttgaaga agaatcgcat gagagaagcg gaagaggcgg aggggatcgc gccggaagat
     2401 ttgcgcgtgc tgctgtggga cattgacggc acgctcctgc gttcgttgcg cgccggggcg
     2461 tttaaggact acaccgtgcc cgtgctggag agcctcttcg ggacggcggg cagtattcac
     2521 gagatgcgcg tctccgggat gaccgatttg cagatcatct gcgaagccct gcgcgacgaa
     2581 ggcatcacga ccgagcaggt gcgtgcgcgc gtcggagaaa ttcgcacacg ctttatggcg
     2641 gagatggagc gcgtcgccac gcgtgcggcg caggatgaaa gcccgctctt tcaactcctg
     2701 acgggcgtac gcgaaatcct tgagcgcgtc gccgcacacc cgcgttatcg ttcgtcgctc
     2761 ctgacgggca acatcgaacc cgccgcgcat ttgaagttgc aactcgtcgg actcgaaggc
     2821 ttctttcaag aactccccgg cgcgttcggc gacgattcgc acgaccgacg cgacctaccc
     2881 gcgctcgccg ccgcgcgcat caacgcccgg ctcaaactca gtttgcgccc cgcgcaattc
     2941 atcgtcatcg gcgacacgcc gaacgacatc gcctgcgcca ggtttttcgg cgcgcgctcg
     3001 gtcgccgtct gcaccgggcg tttcgacacg cgcgagtcgc tcgccgcgca caaccccgac
     3061 gcgattctcc cagacctctc cgacacggac tctgtcctca gaactttcga cgccttgtag
     3121 gcacggattt tttcagacct ccctcaccat ccgtcggtcc aagatgataa gttgcaccaa
     3181 tcaagccgac acatccatcg tcgaaaacac gaccggcagg atcgcaacca gccacgcccg
     3241 cctcgcaaac aaaatagtga agcgcacatg agagtcgcgc ttcactattc tatttgcgac
     3301 tgagaattca acgccctctc acacctttca acgaatctcg cgcccgcact cggaggttaa
     3361 tttcagcgta atagctgacg gctaaggccg gtggaaaccg cgttcgatgt cgcgcgtcgt
     3421 gaggcgcagg atggcggggc ggccatgcgg gcaggtggtg ggcgaggaag tggtgagcag
     3481 ccggtctatg agccagcgca tcttctcgat ggcgagcggc atgttcactt tgatcgcggc
     3541 gtgacatgcg agcgaggcgg cgacgcgctc gcgcagcgtc tcgcgcagcg tgccgcgccg
     3601 ctcggcttca accgtctcca aaacttcggc gagcatgttg cgcgcttctg tcgcggggag
     3661 gtctgcgggc acggccttga ccgcgacggt gcggcctgag aggcgcatca agctgaagcc
     3721 gtaagtttcg agttcttcgg cgaccgtgtc gaaggcggcg gcttgcgcgg gcgtgaggtc
     3781 gaaagtttcg ggcatcagca atgcctgcga ttcggcggcg cgcgtctgtt cgagtttgcg
     3841 gtacttgtcg aagaggacgc gctcgtgcgc cgcgtgctgg tcgatgagca gcacgccctc
     3901 ggcgtcaatc gcgatgatgt aactctcttc gagttgatag agcgggcgaa tgttgttcga
     3961 gatcgcttcg acttcaactt cgtacatcgc ccccgcgatt gaattgagcg gcggcaaaga
     4021 cgacgcgctt ccctgtgacg gcgcgggcgc ggaggaattc aaatcacgcg ctccggcttc
     4081 tgtgcgcaac tcgttatccg cgcgcagcct cgcctcgtcg ccctgcgctt cgcgcccgcg
     4141 cgcgcgttcg tccgtcgcga gcatggaagc gtccgggtcg ttcgcgccgc gcacgtcgat
     4201 gtactcctcg cgttccggcg tggccgtccc ggcattgcgc tccatcgtat cgagcgcggc
     4261 gcgcgcgggc ggcggaagcg attgagcgcg ctccgcaaac tcctggctcg acaataacaa
     4321 ctcttcgtcc gtcggcggcg tgaagccgaa ctccatcggc tcctgctgcg gtagttgcgc
     4381 ctcactcttc gcgcgccacg cggcatcgcc gccggacgct tcgacgcccg cccggtcaaa
     4441 gttcgcgaac tcgcccggac tttgagtttc aactcttcgc tcgtccggct cgtctgcgcg
     4501 gcgcacgtag ccgcccgccg cgagcgcacc gcgcacggct tcgcgcacgg cttcggcgac
     4561 tgcgggcgcg cgtcgaaaac gcacttcggt cttcgccgga tgcacgttca cgtcaacctc
     4621 ttcgagcggc acttcgagaa acagcaacgc cgccggatag acgccgtgcg gcagcaccgt
     4681 gcgatagcct tcgctgagcg cgcggctcac tagacggtcg cgcacgtaac gaccgttgac
     4741 gaacagatat tgcgcatcgc gcgaggtgcg acgctcgcgc ggcgcggaga cgtagcctga
     4801 gacgcgcgca acccgctcgt gtccgccgtt gacttccaac aggctgttga gaaagtctgc
     4861 gccgaagatt tgataagcgc gctcgcgcaa atttgcggcg acgcccgcgc gcagagactc
     4921 gcgcccgttg ttcgtcagga cgaacgcaat ttccgggtgc gcgagcgcgt agtgtgtgac
     4981 gaggctcgtc aggtgaaaac tctccgtcgc ttccgagcgc aaaaacttgc gacgcgcggg
     5041 gatgttgaag aagaggtcgc gcacggtgat ggtggttccg cgcgggcggg cggctggctt
     5101 cacgtcgcgc atgcgcccgc cttcgacgac gatgcgcgtg cctgccgcct cgccctccgt
     5161 ctgcgtgatg agttcgatgc gcgcgaccga agcgatggac gcgagagctt cgccgcgaaa
     5221 gccgagcgtg ctgatgttgt tgagatcatc cgccgtgctg atcttcgacg tggcatgacg
     5281 ctcgaaggcg agcagagcat cgtcctgcgt catgccctcg ccgtcgtccg agacgcgcag
     5341 gagccgccgc ccgcccgctt ccacgtccaa ctcgatgcgc cgcgcgcccg cgtcaatcga
     5401 attctcgacc aactctttcg ctaccgacgc gggacgctcc acgacttccc ccgccgcgat
     5461 ctggtttgct aaatgatctg aaaggacacg aatctttgac atagaggctg ttctttgccg
     5521 gttgtcagta gtcagttgtt caatccggct tttactaact ggcttgattg aaacaactga
     5581 ctactgccaa cgaaccatgg gcgtttttat aagtaaacaa tctgatgctc gaccgagccg
     5641 agatcaatcg cgtcgtaaat ccagtcgaga cagtagcgac cgtggcgcgc aaggagagag
     5701 gtgatgttga gatgtcgctc ctgcaaagtc ttctccgggt agagggcggt gtaggcgcgt
     5761 tcgagttggc gatgggcggc gcggtcgcgc gccatctgcg cgcggtgaaa gcgcgtgcgc
     5821 aggccgccga gttgatgctc gatcttctgc gtgccgtgcg tgagcgcgtc ggcgagcgtc
     5881 gggtcgaagc ggcgcaggtt gtcgccgagt tcgccgagcg cgctgtgaat agaggcttcg
     5941 gcgcggtcga acgcctgcgc ctgctccgcg ccgaggtgct cttcgacgac gcgcgcgatc
     6001 actgcgtcga gtccggcgaa gaagtctgcg agttgcagat tgtagcgttc gagcgtgcgc
     6061 cccgtgcggc gttcggcgaa ggtgagactg gcgcgatgca ggatgggcgt cgcgggtctg
     6121 tcgagcaggc gatagacttc gccggtctgc gcgaagtaag cgatctcggc tgcgccgccg
     6181 aagtaagcga tggtcggcag gagatagtcc tgcacgacgg cgcgcagcgt aacgttcggg
     6241 ctgaagcgtt caggctcgct cgcggctaac tccgcgagtt cttccgccgt gtaggtcttg
     6301 ccgcccgtcg cgcctttggc ttgataaagt ccttcgctgt ttcgtgtgat ggcgcggcgc
     6361 gagccttcga ggtgaatgaa gagggggaaa gcatcgggcg aggtgtgcac ctgcgcgtga
     6421 tagttctccc gttcgagttc gcggctgcgc gcttcgacgg cggcggcgat ctcgggcgcg
     6481 cgtcgcgcgg cctccgagta gagcggcgcg gccagtcgtt tgagcgaagg gtcgagcggg
     6541 tctaggagaa cgagtccgtg acgactgaca agggcggtca ggaagcgtgc gaaggcttcg
     6601 ccgtgcgtgc gaccgggcgc gtaagcatcg cgcaacaaac tttcgagatc agggaggaac
     6661 tcggtcgtcg gcaacaggtc gagcaggcgt tggacggcgt cgctcacggt ttcgtcgagg
     6721 cggacgccgc cgacgggtgc gccttcgcgg tggagcgcat cgggcagatt ggtcgaggcg
     6781 aggcgaccgt cgcaggcagt gaactccgcc gcctgcactt cctcccaatc gtgatcttcc
     6841 gtcgccatcc agaagacggg cacggcctcc gtgccgcgct gcgtcaggca gcccgcgagt
     6901 ttgacggcgg agagggcttt atagatcgtg tagagaggcc cggtgaagag gccgacctgc
     6961 tggccggaga cgacggcgat gctcgcgggc gaacgcagcc gcgcgatgtt gcgctctgtc
     7021 tccgcgcccg cgccccaacg cgtgttcatc tcttcgagcg cgtcgcagag cacgttgcga
     7081 tcagtcttgt gcgcggcgag aacttcgggc gcgcgtgcgg cgagttcgtg atgaaagcgc
     7141 acggcgttcg ggtagtaacg ccggagcgcg gtcgggtcgg cgaggtaatc gagaaagagg
     7201 cgcgactgtt gcgggatgcg cgcgaagggc agagtctcga tgcgcagccc ggcctcttgc
     7261 ggcgtgctga aacaggcagg ttccgtttcg gcggtggggg tcaattcggt gctcacgtcg
     7321 tagctccttc ggcctctagc ttcgaatagt gacaagtgac gactgacgag tgacaagcca
     7381 ggaaagtcgt gaatcgtgaa tcgtgaatcg tgaatagaag aggacggttt ttatctattc
     7441 acgattcacg atttagtttc ttctctcgtc actcgtcact ggtcactgct ttcacatacg
     7501 gacgacactc cgccaacgtt cgctcgatct cgcgtcgcgc cgtctcgtcc ggcaggcgga
     7561 gcggggcgcg cacttcgccg cccgcgtagc ccgcgaggtc gagcgcgtac ttcaagccgc
     7621 cgatgccgta gcgcgtggta acggcgcgtg caagcgggat caacttgcgt tgaacctcgc
     7681 gcgcttcttc gtgtcgtccc tcttcaaaca gccgcgcaat cttcacggcg acgcccggcg
     7741 cgacgcagcc gacggccagt atcgcccctt gcgcgcccgc cgccaaagcc gcgtagagca
     7801 cggaggcgtg tccggtaagc accgcgaaat tgtccgtctg gagactgagc atctccatga
     7861 agttgaccat atcgcccgaa ctgtctttga tgccgatgat cttttcgtgc tcgctcaaac
     7921 gcgcgattgt ctccggcgat agggcgatgc cggtattctg cgggatgttg tagagcacga
     7981 cgggaacgtc ggacgcgtcg gccacggcct tgtagtaatc cgtgagagcg gcgggtgtca
     8041 tctgcccgcg ataaaaatgg ggcgtgatga gaagcaatgc gtctgcgccc gccgcgcacg
     8101 cgcgttcggc ctccgcgatg ctgcggcgcg tgtcgtgctg gccgactccg accacgaagg
     8161 cgagatgcgc gggcacggag gcgcgcgccg cctcgacgac ttcgcggcac tcgccctcgt
     8221 ccagatgcac gcgctcgccc gtcgaaccga gcatgacata accgctcacg cccgtctcgt
     8281 tccaacgctc gatgttcgag gcgagtgcgc gcgtgtccgg acggccatcg gagtcgaagg
     8341 gcgtggtgca gggcaacagg atgccgcgca gtctcttcgc ggtgaggccg ccgtcgcggg
     8401 agtctcgcag atgaattgtg tcgtgtgatt tcaaacgaag gcattataac gaccgcgccg
     8461 gagggcgtgc aacgccgcat cgcgttgaag ccccgcgcgt ggcggcggat ggcagccacg
     8521 cgcggggctt caacgccctg tcttcaaccg gacgtgtcgg ttatgaaatg ttgaaacgtt
     8581 tcttgagatc ggtcggttgc atggcggggg gcacctgata gtaggcgcgt ttgattaagt
     8641 tgtcggcatc gtcggccatg agcgggaggt cgcgcagggg gagatcgcag atggtctgga
     8701 tgacgtgtga gccgagcgcg cgatagcttt cgagttgctg ctcggtgtag ttctggtcgg
     8761 cggtgctttc gtgcggaaag gacgcgctgg cggtggcgta attgagcacg tcgacgggtt
     8821 cgccgccggt gatggcgggc ttgaggtaga tgatcacgcc gtctttggcg ggcgcgccgg
     8881 agaggtcttt gtcggcgcac gaatagcgta tgaggccgac ggcgcagtat ttggtctgcc
     8941 ccttgtgcgc cgcgtcgtct ttgccgtaga tgggcatgcc gccgatgaag tcgatgggga
     9001 tgccgaggtc gatgcgaatt ttgcgcaccg cgcccgcgag gtcttcaaaa cggctctgcg
     9061 ggtcttgcga gccgtcgctc acgacgatga ggtgcgcgcg gcgcaagacc atctcgtaga
     9121 ttccgaggtt ctcgaagtgg ccgccgtcgg agagatagac gtaggggttg atgttgtcgc
     9181 tgtggccgag agcttcggtg agaatgggac gcagggcgaa gcggggtccg gaacggttgt
     9241 aggaatgaaa gaagcgtccg ttaccggcgg gtcccgggtt gccgagccac cagccgagcc
     9301 gcgcgttgaa gagcgtcatg aggaaggcga gcgcgggcga agagtgatag cccatgttgg
     9361 ggctggccgc cgcgccggag atcgtgaccg ccgtgccgag actgatgccg tcgccggggg
     9421 gcgcgccttt gccgccatac tcacgcgtgc gccggtagcc gaggtgaaaa ctgccgcagt
     9481 gtagcggcgt gacggtaaac gtctcggcct tgcgctgctg ccacgcgagg ttgtgcccgc
     9541 tcgtcaggtt gagacagatg ttgacgacgt gcagcagttt ggtcttgcgc gcttccttgt
     9601 cctcgcgccc tttcttatct tcgagcgcgc ccgcgaagtg gctcagttcg tgcatcgcga
     9661 tgttgtcata cgtgtcgaag ccggtgaacg ggttggggcg tcgttccgca cgcgggcgcg
     9721 acgcgcccat gtaggcgcgg atcaggcgat tgcgatacat cgaatggagc gagaacttgt
     9781 tgacgttgac gaaggccgcc atgacgaggc tgatggtgac gagcacgaag gtcaggagca
     9841 ggagcaggtt gagcggggca aagtctatga tgttcaggag gccgtaggaa tcgtaggaga
     9901 gccgctccgc gtcgtgccag ttaaacgacg cgggcacgag ccttccgccc gccagacgaa
     9961 cgccgagcga tctgatgagc aggcttgtcc cgagcgagaa gacgatgagc aacgtcaggg
    10021 cgaagatggg ggcggcgagc ggcatcaggt aagcggccag tccgcccgac ttcccttcct
    10081 gctccgcgtg cgaactcgtc ttttcactct tgccgccgaa aatcgtgaac aggccggatg
    10141 cgccgccgag cgcggtcaga atcgcctgaa gcacgacgga ctgcatgaac acatacagca
    10201 ggccgaccgg agcgaagatg acgagcgcgc tgacgacgct ccacaacaga gagacgataa
    10261 gcacccacgc gccgaaacgc gcccaccact cgcggtcgtc gtcgtccgtg aaattgctcg
    10321 tgaggccgac gaacaaaatt accgagaaca ggaacaggca catgaacagc ggcgcggcga
    10381 agcaagcgta aagttccgtc acgggcgagg gcagaacgcc ggggcgggcg ggcgtgggga
    10441 aagcgatggt ggccgcccac catgtcaccg tgccccagaa cgcgccgatc aaaatgacgg
    10501 ccagcggttc ccacgcgagg cgcggccagt ctcgcgccca acgttctcgc cagaacttga
    10561 aatttttgca gccgagaatg agggtgtaga gaatgtaggc cgcgaggtgg agcgcgacgc
    10621 cgtactgcac aaactccccc caatgctcgc cgagccagtc gccctgctcg ccggggcgac
    10681 gccaagccca cccgctcgtc aggcagatcg ccgagagcga caggggcagc aggcagagcg
    10741 tgaggaacca gccctgctgg ttcaggcgtt ggctgttgat cttgcgcggc gggttcgcgc
    10801 tcggacgcgc cacgcccata tacaccatgg cgatcaagcc gaagattgtg ccgagccaga
    10861 acgcccagtg aaccggcaga cccgtcttaa agttcaactg cacgatgccc gcgacgatgc
    10921 gcggcagcat cagcgcggcc agaatgatgg gaatcagcag cagccagttg agcaccagat
    10981 tccgcacgac gatggcaacg agcgtccacg tgtcggcgga gaggaagccg agcttcgggt
    11041 tgagatagtt gctatagctg cgcaggtggc ggatgggttc gggttcgggt cggagtttgt
    11101 tcttctccgg ttcgttcctg agcgcgccga taacgttcgg caggcgcgag cgatgaatcc
    11161 aggccgacag ccagcttccc agatagccgc cgccggagac tgtggagaga taatcgaaat
    11221 ggccgagcat gccgtagcgc gccaacccct gcacgatgcc gagtccgaaa ctcgcgctgc
    11281 gaatgccgcc gccggagaaa cagagggcgg tgcgcttctc cggcagatcg tgtatgtggc
    11341 gatagagttc cgccgtgaac ttttcgttca acagcacgac gtaacgcgtc ggtcgcatcc
    11401 cgcccgcctg atcatacgac ttctcgatct cctccgtggc gcgttgctca agttccgctt
    11461 cggacacctg cggcgcactg gcctgcctcg gtagtccacg ccggtgctgc tcgcggtgct
    11521 ccgttttctc cgcgtgttcg agcgcgtgct tgatctcacc cttaatctgc tctttgacgg
    11581 cgaggtcaag ccccgccttc gcctgcaaat aagtctccgg gagttggccg tactggtgta
    11641 cgtactcgtc ttccagcacg cggtagagcg gatagaggtg ttgctcgaca cagtgcgctt
    11701 ccagcttttg gtaaggaagg cgcggcgacg tagcggcgaa ggcgttctcg tttggtgaag
    11761 ttgacatctc ttgactccaa actcccgccc ggcggcggcg agcaggtggc gttcacgaca
    11821 cagagtttaa aggcgactaa gcggcacgac ttaactgccg caaacgtgcg gcgcgtctcc
    11881 agctcatggc ggcgggctaa ccgggggctg tcgcgcggag cgtccgccgg actatcgcgc
    11941 gcacgacgca tcggagtctc tcacacggat ttcattttga actcgcaggt gatgtggcaa
    12001 cgcacgtgcg aacgagtcgg cgggtaaatg attgagaacc ggcgatggcc tcacggggtt
    12061 caacttatga actgtgcgga gagaaggtgc gcccgtctgc gcgaaagtaa ggatcgtgaa
    12121 cgcgcgaacg atacggcaag tcagaattaa gtgtcaagac ttttcctgca aacctccggc
    12181 gggtgcgacc gcgacgcgcg cagaggtttg cagaggcgca cggcaggcac acgggagatg
    12241 agcgacgggt tgccgtcggg cgcgggtgcg tgcgagtatt aacgcacatg tcaggagcgc
    12301 gcgacgaacg tttggacttg ctgattatcg gcgcaggacc cgccgggttg tccgctgcgg
    12361 cggcagcggc gcgcgagggc ttgtcgtatc tcgtcatcga aaaaggattg atcgccgaca
    12421 cgatctatcg ttacccggtc gggcgcacgg tcttctccac gcccgacgaa ctggaactgc
    12481 gcgaagacag cctgcatccc tgccgcgaca agccgacgcg cgaggaactg ctctcgcatt
    12541 acgtccgctt cgcgctcgac gaacgcctgc gcgtcaacac ggaagagggc gcggaggcca
    12601 tcgagcgtga cgggcagagc ggcctgttta cggtgcgcac cacgcgagcc gactacacgg
    12661 cctcgcgcgt cctcgtggcg acgggcgcga tggcctaccc gcgccgcttg aacgtcgcgg
    12721 gcgaagacct cccgaaagtc agccatcgct tcgtcgaacc gtatccttac gtgcgcaaag
    12781 aggtgctcgt cgtcggtggc ggaaactccg ccgcagaggc cgcgctcttt ctctgcgaag
    12841 agggcgcgcg cacctcgctc gccatctggc gcgccgactg ggagcaacgc gatcccaaaa
    12901 tgaacgccat caaacactgg gtacgcaccc cgctcgaacg cgaactcgcc gagggcagac
    12961 tccaacttct cttcttcacc cgtctcgaag agatcacgga acgcgaggtg cgcctccaga
    13021 tggaagacgg cgcgacgcgc acggttccca acgatgcggt cttcatcctc atcggcgcgg
    13081 acgccgacca cactctgctc cgacgcgccg gagtcgaatt cagcaacgcc ggactcgccg
    13141 aagcgcccgt ctacgacccg gagacttacg aaaccaacgt ccccggcctc tacgtcgccg
    13201 gtcacttcac acacgcgcgc cacatcaaac aggctatcgc cgtcccgcgc ctcatcgtcc
    13261 cgcgcatcgc tcaatctctc cgcacgatgg tctaaacgcc ctcgaatccg tcccgctcgc
    13321 agacctctca atccgcgccg cccgataatc taccagcggc catgaaaatt ttattgacag
    13381 cgtaattagc ttgaactaac cttcgcctgc aactgcgtca gagcaatctt tacgacctct
    13441 tttccgatga tcaaaagctc agccctcgct tttccaaact cccgctctca acgcttttta
    13501 tactgaaagc actgcacgct cttaagcttc ggcgacagat gcactttgcc actcttctct
    13561 cccctcgcgg cggcagataa cttccagaac cgccgaccct tcatgccaga gagcatatta
    13621 tgaaaagaat caactccgtc acctacgaag ttctccccgg cgaaaagctc tcgatcaaag
    13681 tcactccgac caatttcgga ccgagcgcgc ccgccgtcga agccgacctc gacggcacga
    13741 ccctgaccaa caccggaacg aagagcaagc cggtttataa gttcaccgtt aacaagcagg
    13801 acggagagac taatctggtg acgatggagt tcgtcttcca gttcgactcg cccgacgagt
    13861 ctttctatgc cgtggcgatc tcaggccaga acgacgtggg ctgtccctgc ggctttacgg
    13921 tcgccaaacc ggacgaagat cacggggctg agatcgcttt cgatgttgtc gaagaagaag
    13981 agtaggacgg ggaatcgagt cctgtcctgc gggcgtcggc gaaaggttcc ggcgcaggag
    14041 ggtttcatga agagatacgc gagcctaaaa ctcttgctgg ctctgctcct ctgcctctcg
    14101 cctacgggca gtgcgcagac cgcccttaac tgtgatcagt tgcgggagaa gatcgcgcag
    14161 ttggagaaga tggacctcaa ggccatgtct ccttcgatcc agcaaatcta cagagagtct
    14221 ctgctcaagc tgcacacaca attcggccag tgccttcagc gcgacatctc agccatgacc
    14281 gacatgcaga aggccgtcgc cgggacagac gccgccccgg acgttgaaga caagctccgt
    14341 ggattgcaaa aagagagagc cgagacggac gccaagctcg cgctcctgcg cacggcgtcg
    14401 aacctctcca ccccgcagtc aactgcggac gcgccccccg cacccacgga gacggcttcg
    14461 agcgacgaac ccgcccgcga acagcccgac gcagacgacg ccgtcaacac cgacccggca
    14521 tcgcccgccg gtgcaactac gccgtcgccc gtcgccgtcc agacgaccgg cgcggcgatc
    14581 acctgcgcgc cccccctcac ctatgccgac ccgccgtcaa tactgtccga catcgcaggc
    14641 aaggatgcgg cggacgtggt gagaaacaac gacccggatc gagcgatccg ttccctctcg
    14701 caaatgattc tctacaccct ctccgacgcg gcctcgcccg ccagttcgga gatggtgcgc
    14761 acgctggagg cttaccagtt tctcggcgag acggcgcgga cggacaaaca actcggggcg
    14821 tctgccaagt ccgacggcgc ggtcagcgcg attgagaaac cgggcttcgc gcggctgctg
    14881 ggctttgcca tcgaacacgg cggcatcaat aagaagaacg acggcacgaa cctcacgctg
    14941 agcacctcgc tctattcgct ctacaccttc aaccggggcg atacggcgga aacctacgac
    15001 cgcgcaggcg tgctcaaccg ggtcggcgtc gccgcgacat tcaatataga caacacggac
    15061 gacgaactgg cgaacgcgcg cagaaacaac ctctccgagt ggtcggtcaa ggcgcggctc
    15121 tttggagatc gcagcacgcg ttcggccagt ttccaaaagt tcttcgacgc agagatcagg
    15181 ccgctcatca gggcgcggct ggcttctctg ggcggcgcaa ccaaccaact ctctcaacgt
    15241 ctgcccgtct tcaatcagat cgcgcgggcg gggagaaggt gcctgcccac gcaagtcagg
    15301 acgcgcatgg acgcccccga ttacaagagc gcgacgcccg aagcgcagca gaagatcatc
    15361 agcgatctga tcctcggcca cctgaaagcc aacgtgctcg accgcgttgc gaacggcacg
    15421 ctcaaactcg acgcaccgga tgtcgccttc atagaggttg agttcctgcc cggtctgaaa
    15481 accgcgattg gcaacctgct catggggcgc gaccttttag agaagaggat cgaagacctg
    15541 aaaaagtcgc cgctcggaac gctcgcctat accaatcaca ggcagccgct cgcctcggac
    15601 tactcggagg ccaagttcct gttcgagcag gacaagagtt tcttccgccc gttgaagctg
    15661 atggggaatc tgggagtctc cttctatcat agccccgacc gcgccctgaa ccagcagaaa
    15721 ctccgcgaca tctccgccgc tatctctttc gaaggcacga ccgccagccc cttcacggag
    15781 gcggagaacc tgagcaaaat aacctactcg ttcgtcggtc gctacgagcg gctcttcgag
    15841 aacaggcgca tggcgaatcg taaaccagac ctcgccaccg cacagttcgt catggaaatc
    15901 cccttcatcc gtggactcag cctgccgttc tccctgacct acgccaacgc gacggaggag
    15961 gagcggaaaa agaatgtgcg cttcaacttc gggatgcggc tcgatacgga caagcttttc
    16021 gacctgctgc gcgcgccgtc ggcctcgcgt tgacccggag aattgccccg ccccggagct
    16081 tgcacgcgcg cggcggagat cgcacgacgg acggaaactg tcttgagaga cgagagagcc
    16141 ggggtcagag agcgactccc ggctgtttgg cgttgaacgt aagatggagt ttcgcgtcta
    16201 ttcggactgt tatagggcgt gggggcaaac ctttacacga taatgggttg agtctatact
    16261 gcgtccctgt tcggttcgcg ctctgttggt tttactcaaa ggagacttgt catgcgttgc
    16321 ctggtcaaac gactctcggc tttttcagtt ctctttctgc tgcttggcgc agttgagagt
    16381 cgcgcgcagg tcatgacgat caagagcggg ggcatcgtac acagcggcaa cccgatggtt
    16441 tcacctgtca cgccccacgg cttctacttc aacggcgatg gcatcactgc cgggagcagt
    16501 gatgaaagag gcgcgtcggt gggaatggac tgcaacccgt gcgcgcccgg ccaaaccgtg
    16561 aggctgaacg tcaccatcag ccggttcacc gttcctgccc acggcagcgc gacgcttaac
    16621 gaccttttct atactaacgt ctggttccgt ggcagcgagt tcaagttgtc aaccacgacg
    16681 gtcactattc cggatggtga cgcgcccgag gggttgaagg cgcgctttac catgaccggg
    16741 aatctgatcg gctatacctt cccctttacc agcccgctta tcaatcgaga ggtggagggt
    16801 cagggagcgg tcaggattta cttcaccaaa tctcatcttc cagaccgccc cggctacatc
    16861 tttcaacgcg cgatatacaa ttttgaagcg cgccccgcac gcagccggaa agaggcttcc
    16921 ctgccttcac gcccctctcc cctggcgcag cagtaacaac cccctccaac tttagctgcc
    16981 gcccgttcat tacgtggcat cacctgcgca tcacctgcgc atctgccgcg ccgtctcgcc
    17041 agcgcgtcac gccttcgtaa gtctccttaa caattcgcct ccggcacgcc tctccgctag
    17101 agcgacggct ctacctgcgc gcgttcgcgc cgcgcgacgc ccgtagcgta agcggcttct
    17161 tctacttcgc gggagacggc ttcggcgata cgtttgtcga aaaccgaagg gacgatgtat
    17221 tcggggtgga gttcttcgtc ggagatcagc cccgcgatgg cgttggcggc ggcgagtttc
    17281 atctcttcgt tgatgcgcgt ggcgcggcag gcgagtgcgc ctttgaagat gccggggaag
    17341 cagagcacgt tgttgatctg gttcgggtag tcagacctgc ccgtagccat cacggcgacg
    17401 atgtcggcaa cctcttcggg catgatctcc ggcgtcgggt tcgccatggc gaagatgatc
    17461 ggctttgcgg tcatccgctt cacgtcttcg cggctgatga cgcccggcgc agagagtccc
    17521 atgaagacat ccgcgcccac tatcgcatcg tgcaccgtgc cgcgcatgcc ttccgggttg
    17581 gtgttctgcg cgtaccactc cttgacccag ttcatgtgct cgcgccgccc gcgatagagg
    17641 atgccgtgct ggtcgcagcc gatgatgttc gacacgcccg ccgccatgat gatcttcgtg
    17701 caggcgacgc ccgccgcgcc cgtgccgttg acaatcactt tcaactcgct catcggcttg
    17761 ccgatcaact tcgcggcgtt aatcaacgcg gcgaggacga cgaccgccgt gccgtgctga
    17821 tcgtcgtgaa agaccggaat gtcgagttcc tctttgagcc gttcttcgat ctcgaaacag
    17881 cgcggcgcgg agatgtcttc gaggttgacg ccgccaaagg cggtcgagat gagtttaatc
    17941 gtgcggacga tctcatccgg gtctttcgtc gagaggcaga tgggaaaagc gtcaacgccc
    18001 gcgaactctt taaagagcat cgccttgcct tccatcacgg gcatcgcagc ggcggggccg
    18061 atgtcgccga gtcccaagac cgccgtgccg tccgtgacga cggcgaccgt gttgcgtttg
    18121 atcgtcagcg tgaaagcctt ttcggggtcg cggtggatgg cttcgcagac gcgcgccacg
    18181 cccggcgtgt aggccatcga gagatcgttg cgcgtcttca gaggcgtctt cgagacgact
    18241 tcaatcttgc cgccgagatg gaggagaaaa gtgcggtcgg agacgttgat gacttcgacg
    18301 ccgtctatgt cgcgcgctgc ttcgacgatc tctttgacgt gttcgccgga agaggccgtg
    18361 aaggtgatgt cgcgcgtgat cgaatcgcgc gaggcgttga cgatgtcaat tgcgccgatg
    18421 tcggctccgg cgccaccgag ggcggtcgtc aactctccga gcgtgccggt gcggctcgat
    18481 agtttgacgc gcagggttga actgtaactg acgcttggcg tgttcggcat cgcggctgct
    18541 gtcgaatcct ttccgaagtt gaagcgtgtc tggggaatta ttgatgcccc aactctaacg
    18601 ccaaaagagc gcgccgcgca agcgagagcg ccggacgctc gccctctcgt tcaaagaagg
    18661 tcgaaa
//
LOCUS       DASWNS010000551         7068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_383762, whole genome shotgun sequence.
ACCESSION   DASWNS010000551 DASWNS010000000
VERSION     DASWNS010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383762"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VGB76_19055"
     CDS             complement(<1..639)
                     /locus_tag="VGB76_19055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0101032.1"
                     /translation="MKRSPLLVIFITVFIDLVGFGIVIPVLPYYVEGTSFNATPRTVG
                     ILFASYSVMQLIFSPILGRLSDKYGRRPILFFSLLGTSLGFLILGFATTLWMLFLGRI
                     IDGITGGNISTAQAYIADVTTPENRAKGMGLIGAAFGLGFIFGPAIGGILSQWGIGVP
                     FFFAGGLAFANAILLYFTLPETVTPDHPARMSAANGRWSQLLEAVRQPRLAYV"
     gene            complement(740..1183)
                     /locus_tag="VGB76_19060"
     CDS             complement(740..1183)
                     /locus_tag="VGB76_19060"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0101033.1"
                     /translation="MFQNLIATTAAWFTLPIRLALGLIFIGHGAQKVFGAWGGPGWAK
                     FTTGMEAPFTFMRPSWLWLAMAAIAELVGGVLVLLGLLTRVGAFFLAVTMVVAMAGVH
                     WGAFFMPRGIEYTVALLGMSLALLIAGGGRFSVDERLQAPPRRRW"
     gene            1635..2351
                     /locus_tag="VGB76_19065"
     CDS             1635..2351
                     /locus_tag="VGB76_19065"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101034.1"
                     /translation="MEETNSRQLVVTRVREQGREIRSFDMRPLPSASGEEARVEYLPG
                     QVALLRTGADARPCYFAFASAPEDEELEFLIKRGGGGDGAQALYQLEEGAHVELTRIV
                     GHGFPLDAHKGKDLILIAMGTGIAPLRSTLRHALSRLDDFGQMVVLYGARTPGDFCYR
                     DETETWKAAGVELRQVISKPDGYEWAGSTGYVQSLLDNVLPGLTEPLALVCGSPEMIT
                     HTRDRLQEMGLPPEAILTNY"
     gene            complement(2533..2655)
                     /locus_tag="VGB76_19070"
     CDS             complement(2533..2655)
                     /locus_tag="VGB76_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101035.1"
                     /translation="MGEFDMEAVAAVAAAGRKDRGGVTLEDGIGNERIERELIL"
     gene            2844..5951
                     /locus_tag="VGB76_19075"
     CDS             2844..5951
                     /locus_tag="VGB76_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101036.1"
                     /translation="MNRSTLSRKLAAISLLTTLLTQTVPASVGMGTRNVDRVNPPSFA
                     KEAGGVANTSSALPQSTPAPQLPEGRINPPAINQGEINKAVAFIAPRCDDKWTSKKLR
                     IFQADKDLLAQSGIEISENNADFSSTNIKRCKAVASIDINEDTKLGSYRIRFYPDNAE
                     GEMVEAMLEVTGKDVEQTAAVQDDKENGITVGKPKQFDERTLTLMLQGLESKLAQTQF
                     PDTGGLYGSLGRFGGATANTSSMALSVRGPSLAATTTTIGGGTKDITNSNVLEEGSQT
                     TTGNINSNITNAGGVTTTINADGTITVTSGKTSNSSGSTGEVTSNNQQVTNQAAMAPP
                     VPALPGQTSLFSYQPQFGIAAQDLLAEQTSLFYQIVNLRMLLDRSLTDRIKVKPGIPG
                     TPNGGQMASTEYIRDQVVIGFQISINAAHKDAVAEAEITIDNDDVSLVSLLPQEKTYN
                     VASVTKDSKAIDAGAVVQFVGVGGAIGKTQESMYLVKDTDTVALERRSTGKKVKFAWQ
                     FRPVLGRRTVEPGVRQVYALISVPNILATDGAWKGRVTAKTSWRKYDKKTKTVRDSVN
                     GAEGISQYVDAALPIGNANLTDSGLQPRIDNLSYEDVGNGQLLIVARGQGFTRDTNVV
                     LGNKVLSRPEDGLTIANERRLMLLATAQSLANSSPLMVSRYGTTSFKRSQCFAYPDSG
                     GCRMVDNPYFAHSLEVRKVKARDSLNSEVTVRLVSDDKEISDSAGRTSRYIENFKYLF
                     DNHPPVVVIGGKVFGFSDAPFISRRYKDSITSKDRTTPKDSTAPAAPKESATPDASLP
                     DCADNKWCADLTFIAPTQLLADNTSLTFKELLWDKGALTVELPKQEHFIASTLTTLGT
                     NGEKTQLAISGGGFTNEVKVYVGNTEFSLNCGATATTCQPLKLNLVQGETTGTFITLS
                     PTAAQIKDVKHILIMQGTAQPQTIALPSPIAKVPKAKIISPSPLEISPGSSRREKLQG
                     ANLESIKEILTLGGDKLLFELDEKDKTILFLEIPKSLTTASGEMQIKLVLKDESVEFF
                     TIVVK"
     gene            6229..>7068
                     /locus_tag="VGB76_19080"
     CDS             6229..>7068
                     /locus_tag="VGB76_19080"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0101037.1"
                     /translation="MRNNMRSLVAVWVTVCALIISHSAGMTGRTASAQSNRASHAHAS
                     SGAQGKKVSSDLREQLSKARSGDELVRVVLQDTGETGGNLSSLLRRNGARVDRHFRNL
                     DTRVVEVPVSMIDELAADPDVSYLSPDREVRSSGHVSATTGADAARPASLLGANSALD
                     GSGIGIAVLDSGIYTGHQSFKGKEGVSRVAVNVDFTGAKRMSLADNFGHGTHVAGLAA
                     GNSSLYSGGYTGIAPNSKLINLAVLNSQGKGNTSWVLNALDWVMNNRTTYNIRVVNMS
                     LGTL"
BASE COUNT         1674 a         2033 c         1995 g         1366 t
ORIGIN      
        1 gacgtaggcg aggcgcggtt gtctcacggc ctccaagagt tgcgaccagc gaccgttggc
       61 ggctgacatg cgcgccggat gatccggcgt cacggtttcg ggcagagtga aatagagcag
      121 gatggcgtta gcgaaagcca gacctccggc gaagaagaac ggcacgccga tgccccactg
      181 gctgaggatg ccgccgatgg ccgggccgaa gatgaagccg aggccgaagg ccgcgccgat
      241 gagtcccatg cctttggcgc gattctctgg cgtcgtcacg tcggcgatgt aggcttgcgc
      301 ggtggagatg ttgccgcccg tgatgccgtc tatgatgcga ccgagaaaga gcatccagag
      361 cgtggtggcg aagccgagta tcaggaagcc gagacttgtg ccgaggaggc tgaagaagag
      421 gatggggcgg cgtccgtatt tatccgagag tcgtcccaag atgggcgaga agatgagttg
      481 catcacggag taggaggcga agaggatgcc gaccgtgcgc ggcgtcgcgt tgaaactcgt
      541 gccctccacg tagtacggca gcaccgggat cacgatgccg aagccgacga ggtctataaa
      601 gaccgtgatg aagataacca gtagcggcga gcgtttcata agagagcaat aagtgatgag
      661 tgatgagtga tgagtgatga gttaaaacaa ctgtcttgtc tttcactcat cactcatcac
      721 tcatcactca tcactcgaac taccaccggc gacggggcgg ggcttggagt ctttcgtcta
      781 cggagaagcg accgccgccc gcgatgagaa gcgcgagcga catgccgagc agcgcgaccg
      841 tgtattcgat gcctcttggc atgaagaagg ctccccaatg gacgccagcc atcgctacga
      901 ccatcgtgac ggcgaggaag aacgccccca cgcgcgtcaa cagtccgagc aggacgagca
      961 cgccgccgac gagttccgcg atggccgcca tcgccagcca gagccacgac gggcgcatga
     1021 aggtgaaggg agcctccatc cccgtcgtga acttcgccca acccggcccg ccccacgcgc
     1081 cgaaaacttt ctgcgcgccg tgaccgatga agatgaggcc gagcgcgaga cgaatcggca
     1141 gcgtaaacca cgcggcggtc gtcgcaatca aattctggaa catcgtttag agagtccttt
     1201 ccgttttcag tcagcccgct cttacaacaa gggtggccgc gttgcgattt ctccggcacg
     1261 ggttgcggcg gcggcgcacg cgcacgaagg aggcgtccgc gcttgatcaa ttccggcgcg
     1321 cggcgcgacg ccctccggcg tccggaaaat tgccccgccg acggctcttc tgcgcccgtc
     1381 ctattgattg taaccttgct ggaactggtg caacgctcac agttttatca tagcttcgcc
     1441 cgccgcgcac taagaaggaa gggaaaaggt taaaggggaa aggcgaagga tgacaggccg
     1501 ggagcgggga aacgtctaaa gacgcgcccc gcgattttac ctttcgccct cgcccttttc
     1561 ccttttcccc tcatcctgat ctacacttcg gcgcacccac agcttcactt gaaaacgccg
     1621 gagcagtcga ttttatggaa gagacaaact cgcgccaact cgtcgtcacg cgcgtccgcg
     1681 aacaggggcg cgaaatacgc tcattcgata tgcggccatt gccgtcggcc tccggagaag
     1741 aggcgcgcgt cgaatacctg cccggtcagg tggctctgct tcgcaccggg gctgacgcgc
     1801 gcccgtgcta tttcgcgttt gccagcgcgc cggaagacga ggagttggag tttttgatca
     1861 agcgcggcgg cggcggcgac ggcgcacaag ccctctatca actggaagag ggcgcgcacg
     1921 tcgaactcac gcgcatcgtc ggccacggct tcccgctcga cgcgcacaag ggcaaagacc
     1981 tcatcctgat cgcgatgggc acgggcatcg ccccgctgcg ctccaccctg cgccacgctc
     2041 tcagccgcct cgacgatttc ggccagatgg tcgtgctcta cggcgcacgc acgcccggcg
     2101 atttctgcta ccgcgacgag acggaaacct ggaaagcggc gggcgtcgaa ctccggcagg
     2161 tcatctccaa gcccgacggc tacgagtggg cgggttcgac gggttacgtc cagtcgctcc
     2221 tcgataacgt cctgccgggt ctgaccgaac cgctggcctt agtctgcggc tcgcccgaaa
     2281 tgatcacgca cacgcgcgac cgcttgcagg agatgggact cccgcccgaa gccatcctga
     2341 ccaactatta aacgccgcgc caaaaaaaaa cttccgctga tccttctccc cgtcgcttta
     2401 cgcgttaatc gtcgagggcg gtttttctct gtgccggaat ccccgccggt gaaaccgccc
     2461 ctcaaatcct agcgatctta aaaaagccgc gtcgccctca tcctctgggg cgacaacgac
     2521 ggcatcttct ccctacagga tcaactcgcg ctcgatacgc tcattcccaa ttccatcttc
     2581 aagcgttact ccgccgcgat ccttcctccc ggcagccgcg acggcggcca cggcctccat
     2641 gtcgaactcc cccaactcat cgcgggcgac atcgaagctt tcgccaaata gtctcgctcg
     2701 aatatttaag acaaagggtg gcgcagcctg tcacatgccg ctccattctt tgtcttatct
     2761 tttttctttg gcggcggcat aaaatgttat ataaagcacc cgtcacatcg ttcttctacc
     2821 aatctcaaac tctcaggtaa ataatgaaca gatcaaccct ctctcgcaaa ttagcggcta
     2881 tctctctatt gacgaccctg ttaactcaaa ccgtcccggc ctcggtcggg atgggaacga
     2941 gaaacgtcga tagggtgaac cctccatcgt tcgcaaaaga ggcggggggc gtcgccaata
     3001 cttcatccgc cctgccgcag tccacacccg cgccgcagtt acccgaaggg cgtatcaatc
     3061 cacccgccat taatcaggga gagataaata aagcagtcgc ttttattgcg ccccggtgcg
     3121 atgacaagtg gacaagtaag aagctgagaa tatttcaggc agataaagac ttgctggctc
     3181 agagcgggat tgagatcagc gaaaataatg cggatttttc gtcaaccaat attaagcggt
     3241 gtaaagccgt cgcgagtatc gacatcaatg aagataccaa gttaggcagc tatcgaatca
     3301 gattctaccc ggataacgcc gagggtgaga tggtcgaagc gatgttggaa gtgaccggga
     3361 aagacgttga gcaaacggcg gcggtgcagg atgataaaga gaacggcatc acggtcggga
     3421 agcccaaaca gttcgacgag cgaacgttga ccttgatgtt gcaaggtctg gaaagcaaac
     3481 tggcgcagac acaatttccc gacacgggcg gactctacgg gagtctcgga agattcggcg
     3541 gcgcgacggc gaacacctct tcgatggctc tcagtgtgag ggggccgtct ctggctgcaa
     3601 ctacgacgac tatcggcggc ggcaccaaag atataaccaa cagcaacgtt ctcgaagaag
     3661 ggtcgcagac gacgaccggc aacataaaca gcaacataac caatgccggt ggggtgacga
     3721 caacgatcaa cgcagacggc acgatcacgg tcaccagcgg taaaacttcc aattcaagcg
     3781 gcagtacggg cgaggtcacc agcaacaacc agcaagtcac caatcaggcg gcaatggctc
     3841 cgcccgtacc cgccttgccg ggacaaacat cgctcttttc ctaccaaccc caattcggta
     3901 tcgccgcaca ggacttgctc gccgaacaga cctctctgtt ttaccagata gttaatctgc
     3961 ggatgctctt agaccgttcg ctgaccgacc ggattaaagt taagccgggc attccgggca
     4021 caccgaatgg cgggcagatg gcgtctacgg agtacatacg ggatcaggtg gtcatcggat
     4081 ttcaaatcag cattaatgcg gcgcacaaag atgccgtcgc agaggcggag ataactatcg
     4141 acaatgatga tgtctcgctc gtttcactcc tgccgcaaga aaaaacctac aacgtcgctt
     4201 ccgtgacgaa agactcgaaa gccattgacg cgggagccgt cgtccagttc gtcggcgtcg
     4261 gaggcgcaat cggcaaaacg caggaatcaa tgtatctcgt taaagatacg gatacggttg
     4321 cgctggagcg tagatctacc ggaaagaaag tgaagtttgc gtggcagttt cgcccggtgc
     4381 tcggacgcag gacggtcgag ccgggcgtga gacaggtcta cgcgctgatc tcggtgccga
     4441 atattcttgc aactgacggg gcttggaaag gtcgagtgac ggcaaagacc agttggcgca
     4501 aatacgataa gaagaccaag accgtgagag attcagttaa cggagcggag ggaatttctc
     4561 aatacgtcga tgccgcgctt cccatcggta atgcaaattt gaccgattcc gggttgcagc
     4621 cgagaatcga caatctcagc tatgaggatg tcggaaacgg gcaactatta atagttgcgc
     4681 gggggcaagg atttacgcgt gataccaatg tcgtactggg aaataaagtg ctcagccgac
     4741 ctgaggatgg actgaccatc gccaatgaaa gacgtctaat gctcttagcg acggcacagt
     4801 cgttggcaaa ctcttctccg ttgatggtca gccggtatgg caccaccagt tttaagcgta
     4861 gccagtgctt cgcttaccct gattcaggcg gatgcagaat ggtagataat ccatactttg
     4921 ctcattcact cgaagtccgt aaggttaagg cgcgcgactc gctaaattca gaggtgacgg
     4981 ttcgattggt ttccgacgat aaggagattt cggacagcgc cggtagaacg tcgcgttaca
     5041 ttgaaaactt taagtattta tttgataacc atccgcctgt cgtggtgatc ggcggcaagg
     5101 tcttcgggtt tagtgatgcg ccgtttatct ccagaagata caaagacagc ataacttcta
     5161 aagaccgtac aactcctaaa gacagcaccg ctcctgccgc tcctaaagag agcgcaactc
     5221 ctgatgcctc attacccgat tgcgctgata ataaatggtg tgctgatctg acttttatag
     5281 cccccaccca actcttagcc gataatacct ctttaacatt caaggaactg ctttgggata
     5341 aaggcgcgct gacagttgag cttccgaaac aagaacactt tatcgcgtcc actttgacaa
     5401 cgctcggcac taacggcgag aagacgcagt tggctatttc aggaggaggt tttactaatg
     5461 aggttaaggt ctatgtgggc aatacggagt tcagcctaaa ttgcggagcg accgcaacta
     5521 cctgtcagcc gcttaagttg aatcttgttc agggggagac gacgggcacg tttataactt
     5581 tgtcgccgac ggcggcgcag atcaaggacg tgaagcatat attgattatg caaggtacag
     5641 cccagccaca gactatcgcc ttaccgtccc caattgcgaa agttccgaaa gcgaagatta
     5701 ttagcccgtc gcctctcgaa atatccccgg gcagcagtag acgggagaaa ctccagggcg
     5761 caaaccttga atcaatcaag gaaatcctca cgctcggcgg cgacaaactc ctcttcgaac
     5821 tggatgagaa agataaaacc atcctctttc ttgagattcc aaaatcgtta accaccgcga
     5881 gcggggagat gcaaattaaa cttgtcttga aggacgagtc cgtggagttc tttacgatcg
     5941 ttgtgaagta gccacttccc gcgttagccg tcgcaacaca gggcggcctt gaagccgtcg
     6001 ctttcagatt gattgcaggc agagcgcagc ttgaccgatc cggtcaagct gcgctctgcc
     6061 ttttgtcgcc ctccgttaca cggaaaatcc cgaaacaacg agctatttgc cccgcagagt
     6121 cgttggcatc ggaaatgcaa tagcctgttc gcctgcatcc ctatacttag cgaagatagg
     6181 ggcaaggtgt ttacggtttc cccaaacaat cccgaaagat attgagccat gagaaacaat
     6241 atgcgttccc tcgtcgcagt ctgggtcact gtctgcgcgc tgatcatctc acacagcgcc
     6301 ggaatgacag gccggacggc cagcgcacaa tcaaacagag catcgcacgc acacgcctcc
     6361 agcggcgcgc agggcaagaa agtctcctca gacctgcggg aacagttgag caaggcgcgc
     6421 tccggcgatg aactcgtccg cgtcgtactt caagacacgg gagagacggg cggcaatctc
     6481 tcgtcgctgc tccggcgtaa cggagcgaga gttgaccggc acttccgcaa tctggacacg
     6541 cgcgtcgtag aggtgcccgt gagcatgatc gacgaactgg ccgccgaccc ggacgtgtcg
     6601 tatctctcac cggacaggga agtcagatca tcaggccacg tctctgcgac gacgggtgcc
     6661 gacgccgcgc gtccggcgag cctgctcggc gcgaactcgg cgctcgacgg ctccggcatc
     6721 ggcatcgccg tgctcgactc cggtatctac accggccacc agtcgttcaa agggaaagag
     6781 ggagtcagcc gcgtcgccgt gaacgtggat ttcacgggcg cgaagaggat gagtctcgcc
     6841 gacaacttcg ggcatgggac gcacgtcgcg gggctggcgg caggcaacag cagcctctat
     6901 tccggcggct acaccggcat cgctcccaac tccaaactga tcaacctcgc cgtgctcaac
     6961 tcgcagggca agggcaatac ctcctgggtg ctcaacgctc tcgactgggt gatgaataac
     7021 cgcacgacct acaacatccg tgtcgtcaac atgagcctcg gcacgctg
//
LOCUS       DASWNS010000552        11887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_254894, whole genome shotgun sequence.
ACCESSION   DASWNS010000552 DASWNS010000000
VERSION     DASWNS010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11887
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254894"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VGB76_19085"
     CDS             <1..781
                     /locus_tag="VGB76_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101038.1"
                     /translation="PVIVEQLVRIFNNLDSQRKASILKSVSRLCLLLGQDQRGVIGEG
                     ILTGHLQDESNTHHKLSTMADDLWEMGQILAGVTSAGPGEEYASVELKGLIERAISDL
                     EERMYLRGIRFEVEGSCTVRANRDDMYIAVVRVMQWLAQRKIETPAGVEPQITVRCFR
                     EDGTTQIMFEDRSRRLADSLREQLFVPLAVAITLPAGAILQRPAQYLPLYVAKMLIEE
                     KYGGWLDDKSSELPGEIGHRLVMRFETAGNDAGPLDTTAFA"
     gene            1003..1689
                     /locus_tag="VGB76_19090"
     CDS             1003..1689
                     /locus_tag="VGB76_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101039.1"
                     /translation="MDNITRQYRLLRAYESKRDLSDRPKEVESANNEIGAIAQSLAGY
                     IEQYNEICRDEGADCSTEVRTIWEEIQHLRRGQQQIIRKQDETLENIDQTRHRILLEI
                     ENTHRRLALSLLSALSEQEVRVVRSILAEIDSDRLSRQEISEALNAIQIGYEELTAKS
                     LTLVNQTTDADEVQKLWDSPDINAGGKLKLSIPLIPAILSYESELSFSIKQSLKTFWE
                     RYFKGSQVLL"
     gene            1801..6402
                     /locus_tag="VGB76_19095"
     CDS             1801..6402
                     /locus_tag="VGB76_19095"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101040.1"
                     /translation="MSLLTTLQQRQKQNLEEALLKLLELLRRAEEWRDVTSSPSERER
                     YELEVRDLKSSVEQCVADLQRLEEEMQAAASSSESARPSPESYRVSLSDSLTEEQLDS
                     QFQREVRELLGLIEYELSAEAVPGTAPSAAPTFVASQEWDFETVKTLFQCRYGIIDEQ
                     AVMSFSSIYALNQQSLGLSFAIIVTNTYIAPAAKSLARQHRIKLLTFEDLLNTVIKPD
                     NYLKSKCRDYEQNNSLFHTYVELRYLRRVPGRLTAQKATPQEFLSPEAIDGGSFEEKG
                     DLTPYVDAWLLKDGNSQICLLGEYGTGKTSFATQYFYQRALAYLQSPLKNRVPLLITL
                     NRYHKSADIEQLMTDFLVNECGVRRNFNTFLGLAARGKLLIILDGFDEMAQQVDVNVR
                     RHNFKEIAKLAVGRNKIILSGRPNYFLTQAEINESFSQGYEELDPYRAAIRKATAGPG
                     PQYEILNLTLFDQWQIEEFLKRQSDYLKEKGIKDWRELRKVIYDTYNLAELARTPVLL
                     DIIIKTISEIRGLVTHINAAKLYEVYTNFWLVREYDHKGEVRWLIDRKAKELFVSELA
                     WTMLKTDSLRPEIHFSQLSERVRSYFKLEKASEIEYFSSDIRFCSYLIHSASDGNYKF
                     IHKSFMEYFSARYIYRALFEEQDIPRIITDKPVTDEVFFFLSQMVSLADVDVMQTLSR
                     DERDGQRREFLIDLTTRVLQQSVLSSEHRGSLQSAEELTHKLLDYSAEVNSDRGRLWC
                     LLTLGKLKAQLSLYEEAEDCFKEALALSVQLGDVAAEGEALSQTGAIRQRRGEYIEAQ
                     RFYTLAIRKFEEVSDRSRKGQALASVGAIHQHRGEYAEALQFYTDAIKLFEESDDRAS
                     MGRTLASVGTLHRNIGEYNAALEYYQRARAIFKELADRRSEGQAIAHLGHMYQETGRY
                     EEAEGFFREELDIAQELDDQQGISRALSNMGYLCRTTKRYADAEEWYQRALAISEGLN
                     DRQGVGDALYAIGNVYQYERRFDDAEDLYRRSVLILEELKDMRGLGLNYYQMANVYAE
                     QQGRFPESRKYYKKARDIFAELGDRRSISSVIHQLGDLAQRDGDFDGAERYYRESLRV
                     KEQINDAVGIQELVEALGGLEEARQNYGEAQKLYLAALGDAEKVGNLVNACNLHARLG
                     KLAQKVGDEQKAVQHYASYIRLAQEMNRPLLEDVVQEYERLSSLKKSNPFIVGMPLDK
                     GTLFGGRNAEVEAIHRHVQQQNHVMLIGERRMGKTSVLFHLRDRLRPPLIPIFIDLQA
                     FPIQAEGMLGGMIRKVVNELLGRGMLSSEQWSQFSITYARDFVNALGSILDEVKGKLK
                     DVKLVLIIDEADMLFEAEGQTGKVLRSALTMNRDVVAVIAGTTRLLRLSANAQTSPLQ
                     NIFVPMTLSPLNKGETDFLIEEPSRQVRVKYTTSALERVYYLSGGIPFYVQVIGFELV
                     EQAKQVSKDEVGVEDVNRIVPQILGRYTINFQDIWTNLSDREKAVVAEIVNKNVRAGL
                     DEKCISNLQSKQLMIEENGRYRLTFGLFEEWVKSYNAAS"
     gene            6743..7131
                     /locus_tag="VGB76_19100"
                     /pseudo
     CDS             6743..7131
                     /locus_tag="VGB76_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689228.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            7365..>11887
                     /locus_tag="VGB76_19105"
     CDS             7365..>11887
                     /locus_tag="VGB76_19105"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HEY0101041.1"
                     /translation="MSDSDTDLLHVPSWLETPPLASVPPPVETRKQELPFEHLTWENF
                     EKLCVRLARLEADVEHCQLYGVRGQDQEGIDLYAKSKTSGQYTVYQCKREKGFSASKI
                     EGAVTTFLAGTWSGRASTFVLCTMESLVSTGRAEEVERQRTILAAKGVDFVIWDGQQL
                     SLRLKALPVIVDDFFGRTWVEAFCGGNAADQLGGRLDAVRMAEFRLRLGNFYQRLFNN
                     HDPGLPLLASTVSDDPIPLSERYVLPDITDSRSIETEQPIDGAGYHLGLSGLKELRSG
                     DTPRGELLSEKSYPSYKYQNVSQRQRVETWLGANRRCVILGGPGSGKSSLLRFLCLDL
                     FQDEPQLTELARWWGQFLPVWIPFAFWTRKFSTSEGRDVPLTAMLEDWFKGWDEGHLW
                     PIVRQALDDERLLLLVDGLDEYSDEDAARNALLRLKIFIEQRNATAVLTARPRGFARL
                     GMQTAGWGRGDLSDLSEEQQEQLTRIWFTHRERKVRGSAGDDEDVIACRVEAEVQRFL
                     GDLRRSSELRELARVPLLLCLLIAYKILHVQLPQNRFKAYQALVDYLIHTHPERRRLA
                     ALIRETPLALTDEEVKRAFANLAYRIQSQYGTGVVEQTEAIKATTQFLSDESGVIGFE
                     AAEARKVSRELIHTGEDTLGLLVERSQSEIGFFHRALQEYLAAYHLSTISHNERVSIV
                     EEHCVEPQWREVILNLFSILNSIDEVSSYVRSAFKRQVAPHERYEIEQLLAEVVFGGF
                     NVPVGLSREIARTVFRVVETESWMPHRERLLDQAVAGLRSAKTREMVKDKLKVWFPDR
                     TRKIRAEMFSAVAGWPSSADSNQCLLLGLRDEDSTIKRAAAKALAALNQGDEEIGEQV
                     AELAWSEADPRTRAAAIECLMLGWPNHSRLTSIIDAARASVFPRLRLMGINGKVRQGR
                     ATDEDLNQLFYLGSRDAGVDRRWFDFLVESLVKGWTGSPEVKQRCLDAVERTKTAFGA
                     MSDFGLYGDLGKAVLLSGYPQDNEVAQVLAREVNWGSVSLRDANMRSVDFFDLLAKNF
                     KDHPKVVEAVDRWLRAQKGERKGSGFYRAALVGRTTLAKSLLIDSLHSKHAGLAAQTL
                     LEGWGLQDQDVAQALLSAANGPHEWASQLGEYLPLIITDVDICRRRLLELISHAPPSA
                     VDSVLKGLASLGETFDDAEVVDAVLQIPVDNYGHQPGHKALIKYYPWDSRVRELAKDV
                     LNNPDDRLSAVATAYGADDYFRSRIIELMTPLPAHLRKVMAQRLGEEVGDDFSLTLLE
                     SYNDEADADVRVQASISYHSLLKTLGRDTDAAIKVLSNRIVDYSKDWAATRHAAFSGL
                     LVLGRLDVMMNAQEKAPKLEDIRCRFPLSRGEISPSVPLIRLLLKNWVYIRATFGEEF
                     WFRLGYEEERLRSDLWYYIIPFANEYPQPRAEVINYLEASTEKSRFYSPRRLHFLSRA
                     LPKSTLLRQYCMSILAGDENKFDFDGEESVIAAEIIASQFRDDEVVQRWLNENIREDY
                     GEEGVPDNIILLL"
BASE COUNT         2866 a         3009 c         3522 g         2490 t
ORIGIN      
        1 gcccgtcatc gtcgagcagc tcgtacgcat cttcaacaac ctggactcgc agagaaaggc
       61 gtcgatcttg aagagcgtct cccgcctctg cctgctgctg gggcaggatc agaggggtgt
      121 catcggcgag gggattctga ccgggcacct ccaggatgag agtaatacgc accataagct
      181 gagcacgatg gccgacgacc tgtgggagat ggggcagatt ctcgctggcg taacgagcgc
      241 ggggcccggc gaggagtacg cgtccgtcga gctcaaagga ttaatcgagc gggcgatcag
      301 cgacctggaa gagaggatgt acctgcgcgg catcaggttc gaggtcgaag gctcttgtac
      361 ggtcagggcc aaccgggacg acatgtatat cgccgtcgtc agggtgatgc agtggctggc
      421 ccagcgcaag atagagaccc cagccggcgt ggagccacag atcaccgtgc ggtgtttccg
      481 cgaggacggc accacgcaga tcatgttcga agaccgcagc cggcggctgg cggatagtct
      541 gcgggaacag ctcttcgtgc cgctcgccgt tgccatcact ctccccgccg gggccatttt
      601 gcagcgcccg gctcagtacc tgccgctcta cgtggcgaag atgctgatcg aagagaaata
      661 cggcgggtgg ttggacgaca agtctagcga gttgccaggg gagataggtc acagactggt
      721 gatgcggttc gagacggcgg gcaacgatgc cgggccactc gacacgaccg ccttcgcctg
      781 acggccgaat tgagccggtc gcgcagcggc gatatatctg aagcacacga tcaaatcccc
      841 tgcgcacgaa ttgacggccc ctccacgtgc ctttctttga cgccgggcaa ttcgacctga
      901 ggcgccggcg gggcgcacag ggaggagagg gttggcattt aagaacgcag aaagggggct
      961 gtgagcgccg tgagcttcaa agagattcgg aaaagggatt tggtggacaa catcacccgg
     1021 caatacagac tgctgcgtgc gtatgagagc aagcgcgacc tttccgaccg gcccaaagag
     1081 gtcgagtcgg ccaataatga gatcggcgcg atcgcccaat ctctcgcggg ctacatcgag
     1141 cagtataacg agatttgccg tgacgaaggc gcggactgtt cgacagaggt gaggacgatc
     1201 tgggaggaaa ttcagcactt gaggcggggg cagcagcaga tcatccggaa gcaggatgag
     1261 accttggaga atattgatca gactcgtcac aggatcttgc tggagatcga gaatacccac
     1321 cgccgcctag ccctcagcct cttgtctgcc ctaagcgagc aggaggtgcg ggtggtgaga
     1381 agcatattgg cggagatcga ctcagacagg ttgtcccggc aggagatctc tgaagcccta
     1441 aatgctatcc agatcggcta cgaggagtta acagccaagt ccctaacatt agtcaaccaa
     1501 acgaccgacg ccgatgaggt gcagaagctg tgggactccc cggacatcaa tgcgggcggg
     1561 aagctgaagc tttccattcc cctgatcccg gcgatactct cctacgagtc cgaactgagt
     1621 ttctccataa agcagtctct taaaaccttc tgggagcgct acttcaaagg aagccaggtg
     1681 ctgctttaat cggcctcatg agctttgaag ggggttcgtg gccgtcagac ctccggccgt
     1741 cggttcacgc ggcgccctga ctcatcttca cctcggcaga tcgggacagc ggacaaggcc
     1801 atgtcactac tcaccaccct ccagcagagg cagaaacaga acctagaaga ggcgctcctg
     1861 aagctactcg aactgcttcg ccgggcggag gagtggcggg atgtgacttc gagccccagc
     1921 gaaagggagc ggtacgaact cgaggtcaga gacctgaagt cgtcggtgga gcagtgcgtg
     1981 gctgacctgc aaaggctgga ggaggagatg caggccgccg cgagttcctc cgagtccgcc
     2041 cggccgagcc ccgaaagcta ccgggtcagc ctgagcgact ccctgacgga agagcagctc
     2101 gactcccagt ttcagaggga ggtgagggag ctgctcggac tgatcgagta cgagttgagt
     2161 gcagaagccg tcccgggcac ggctccctca gcggcgccga ccttcgtcgc gagtcaggag
     2221 tgggatttcg agacggtgaa gacgctcttt caatgccgct acgggattat cgacgagcag
     2281 gcggtgatga gcttcagcag catctacgcg ctcaaccagc agagtctcgg gctgagcttc
     2341 gcgatcatcg tcaccaacac ctacatcgcc ccggccgcga agtcgctggc acgtcaacac
     2401 cggataaagc tgctcacgtt cgaagatctg ctcaacactg taatcaagcc ggacaactac
     2461 ctgaaatcga agtgtcgaga ttacgagcag aataacagcc ttttccacac ctacgtcgag
     2521 ctgaggtatc tgagaagggt cccggggcgg cttacggcgc agaaggcgac cccgcaggag
     2581 ttcctgagcc ccgaggcgat tgacgggggc agttttgaag agaaaggaga cctgacgcct
     2641 tacgtcgacg cctggttgct taaagatgga aactctcaga tctgtctgct cggcgaatac
     2701 ggcactggga agacctcttt cgcgacccag tatttttatc agcgggcgct ggcgtatctc
     2761 caaagcccgc tcaagaaccg ggttcctttg ctgattacgc tcaatcgcta ccacaagtca
     2821 gccgacatcg agcagttgat gactgatttt ctggtgaacg aatgcggggt caggagaaac
     2881 tttaacacgt tcttggggct ggctgcgcgc ggcaagctgc taatcattct cgacggcttc
     2941 gatgagatgg cacagcaggt tgacgtgaac gtgcgccggc acaacttcaa ggagattgcg
     3001 aagctcgcgg tcgggcggaa caagattatt ctgtcgggcc gccccaacta cttcctgacg
     3061 caggcggaga tcaacgagag cttcagccag gggtatgagg agttagaccc ctaccgggca
     3121 gctatcagaa aagcaaccgc aggccccggg ccgcagtacg agatactcaa cctcaccctg
     3181 ttcgaccagt ggcagatcga ggagttcctg aagcggcaga gtgattatct gaaggagaag
     3241 ggaattaaag actggcggga gctgcgaaag gtcatctacg acacgtacaa cctggcggaa
     3301 ctagcccgca ctccggttct gttggacatt atcattaaga cgatttccga gatacgcgga
     3361 ctggtcaccc acatcaacgc cgccaagctt tacgaagtct atacgaactt ctggctggtg
     3421 cgagagtacg accataaagg cgaagtgagg tggctgatag acaggaaagc gaaggagctg
     3481 tttgtgtcgg agctggcctg gacgatgctg aaaaccgaca gcttgcgccc ggagattcac
     3541 ttcagccagt tgtccgagcg cgtgcgcagt tatttcaagc tggagaaagc ttccgagatc
     3601 gaatatttct caagcgacat ccgattctgc tcgtacctta tccactcggc ctcggatggt
     3661 aactacaagt tcatccacaa gtcgttcatg gaatacttct ccgcgcgcta catttatcgg
     3721 gccctgttcg aggagcagga cattcccagg ataatcaccg acaagccggt cacggatgag
     3781 gtcttctttt tcctgagcca gatggtgagt ctcgccgacg tggacgtgat gcaaacgctg
     3841 agccgggatg agagggacgg gcagaggcgg gagttcctga tcgatctcac cacccgcgtc
     3901 ctgcaacaat ccgtcctatc ctccgagcac aggggctccc tccagtccgc cgaagagttg
     3961 acgcacaagc tgttggatta cagcgcggag gtcaattccg acaggggcag gctctggtgc
     4021 ctgctcaccc tcggtaagct caaggcgcag ctgagcctct acgaggaggc cgaggattgt
     4081 ttcaaagagg ctctggccct ttccgtgcag ttgggggacg tcgcggccga aggcgaagcc
     4141 ctaagccaga ccggggcaat ccgccagcgg cgtggcgagt acattgaggc ccagaggttt
     4201 tacacgctgg ctatcaggaa gtttgaggag gtcagcgaca ggtcgaggaa gggccaggcg
     4261 ctggcaagcg tcggcgccat ccaccaacac cgcggcgagt acgccgaagc cttacagttc
     4321 tacacggacg ccatcaagct ctttgaggag agcgacgaca gggcgagcat gggccggacg
     4381 ctggcgagcg tcggcacgct tcaccggaac atcggcgaat ataacgccgc gctggagtat
     4441 taccagcgcg cccgggcaat cttcaaggaa ctcgcggaca ggcgcagcga gggccaggcc
     4501 atagctcacc tcggacacat gtatcaggag accgggcggt acgaagaggc ggagggcttc
     4561 ttccgggagg agttggacat cgcgcaggag cttgatgacc agcagggaat cagccgggcg
     4621 ctctccaaca tgggatacct gtgccggacg acgaagcgat acgcggacgc cgaggagtgg
     4681 tatcagaggg ccctcgccat ctcggaggga ctcaatgaca ggcagggcgt gggcgacgcc
     4741 ctctacgcca tcggcaacgt ttaccagtac gagaggcgct tcgacgacgc ggaggactta
     4801 taccggcgtt cggtcttgat cttggaagaa ctgaaggaca tgagaggctt gggcttaaat
     4861 tattaccaga tggcaaacgt ctacgccgaa caacagggca gattcccgga atcgaggaag
     4921 tactacaaaa aggcccggga tatctttgcg gagttaggtg acaggaggag catcagctcg
     4981 gtcatccacc agcttggcga cttggcgcag cgtgacggtg actttgacgg ggctgagagg
     5041 tattacaggg aatctttgcg tgttaaagaa cagattaatg atgctgtagg catacaggaa
     5101 ctggttgaag ccttaggcgg gctggaagag gctcggcaga attatggcga ggcccagaaa
     5161 ctatacctag ctgcgctcgg cgacgcggaa aaggtaggca accttgtgaa tgcgtgtaac
     5221 ctgcatgcaa gactaggcaa gttggcgcag aaagttgggg atgaacagaa ggcggtccag
     5281 cattacgcct cgtatatcag acttgcgcag gaaatgaacc ggccacttct tgaggatgtc
     5341 gtgcaggaat atgaacggct aagttctttg aagaagtcga acccttttat agtcggaatg
     5401 cccctcgaca agggcacctt gttcggcggg cgcaatgccg aggtagaagc aattcacagg
     5461 cacgttcagc agcagaacca tgtgatgctg atcggcgaac gcaggatggg gaagaccagc
     5521 gtactcttcc acctaaggga tcgattgagg ccgccgttga ttcctatctt tatagacctt
     5581 caggctttcc ccatccaggc tgaagggatg ctagggggga tgattaggaa agtagtaaat
     5641 gagttgctag gccgcggcat gctctcgtca gagcaatgga gtcagttctc gatcacctac
     5701 gcgcgtgact tcgtcaacgc ccttgggtct atcctggatg aagtgaaggg gaagttgaaa
     5761 gacgtaaagc tggtgctgat tatcgacgag gcagacatgc tcttcgaggc agaaggtcag
     5821 acggggaagg tgctccgatc cgccctgacg atgaaccgag atgtggtagc ggtcatagca
     5881 gggacgaccc gcttactgcg tctttcggct aacgcgcaga cttcacctct tcaaaatata
     5941 ttcgtaccca tgactctgtc acctctgaat aaaggagaaa cggacttttt aattgaagag
     6001 ccgtcccgac aagtgagggt gaagtacacg acctcagcac tcgagcgcgt ttattacttg
     6061 tccggcggca tacctttcta cgtccaggtc atcggctttg aattggtcga acaggctaag
     6121 caagtgtcga aagacgaggt tggcgtcgaa gatgtaaata ggattgtccc ccaaattctg
     6181 ggtaggtaca caataaattt tcaagatatt tggaccaacc tgagcgaccg agagaaggct
     6241 gtcgtcgctg agatcgtcaa caagaatgtc cgggcggggc tcgatgagaa atgcatctct
     6301 aatttgcaaa gcaaacagct gatgattgaa gagaatggca ggtacagact tacgttcggt
     6361 ttattcgaag agtgggtcaa gtcatataat gccgcgagtt agataagtct gatatgtgcc
     6421 tttttcgtaa gaaggataga gtaggacatg tctttaaccc ttataatgta agcatagtta
     6481 atgaaacgca cgagaagcga aatggtgaga aggcagtatt gtctgacaaa ccattcgcgc
     6541 taacgctaat ggagggggga gcttcagcac taaaatttcg caggcagtta tcaatcatac
     6601 gggccaaggg tttaattcgt gatgagttgc ttgccagtgg cagttagcga taaagggcgt
     6661 ctagttggct gtgttgcagg aattacttcc gcagctataa cccgaccttt agctcagtca
     6721 gcacactccg ggaatcatac tcttgaggca caataaatcc tcgttcaaat ggcgtcactt
     6781 tgagcccact atcatccttt tgtgcgtcag atggtattgc agataccaac tctcttatag
     6841 gcctcctacc ccgaggcatt cgccacttct gtgaaggaga aagtacttcc catagattgc
     6901 aagctcaggc gcatcaaata cctgaacaat atagtcgaac aagaccacag ggctatcaga
     6961 aggaggtggc gagcgatgca atgcttccgc tccttccaca ctgccgaacg gacgatagag
     7021 gggcttgagg ctgcggacat gatgagtaag gggcaggtga agaggttagc cggtggagac
     7081 tcgatagggc aggcgaagtt cgtcgcaggg ctgttcggag ttgccgcctg actcaaaatc
     7141 accttgagcc tattctcggc tcagagcatt tgctgcaaca taaccaatac aactacatta
     7201 tcgaaccact ggcagcaggg aacgcaatag ccctcgagtt caacacgcct cctcaccacg
     7261 cacttgtttt gctatgactt gaggcgttaa taatactgcc aatagggcga ccgtcctaca
     7321 tttgcgactt cacgtcatga atccatgatg ctctgagggc ttttatgtca gactccgaca
     7381 ccgacctttt acacgtgcct tcttggttag agaccccgcc gctcgccagt gtccctcccc
     7441 cggtcgagac aagaaaacaa gagctaccat ttgaacattt aacctgggag aacttcgaga
     7501 agttgtgtgt acgtctcgca cgcttagagg ctgacgtcga acactgccaa ctttacggag
     7561 tgcgcgggca ggatcaggag ggcatagact tgtatgcgaa gtcgaagacg agcgggcagt
     7621 atacagttta tcagtgcaaa cgcgaaaaag ggtttagtgc aagcaaaatt gaaggtgcag
     7681 tgacgacttt ccttgccggg acgtggtccg gcagggcctc gacgttcgtg ctctgtacta
     7741 tggagagcct cgtcagtaca ggccgtgcgg aagaggttga gaggcagcgc accatcctag
     7801 cagcaaaagg ggttgacttc gttatatggg atggtcagca gctatcgctg cgcctgaaag
     7861 ccttacccgt aattgtagac gattttttcg gaaggacgtg ggtggaggca ttctgcggcg
     7921 gcaacgcggc cgatcaactc ggtggtaggc tagacgccgt aagaatggct gaattccgct
     7981 tacgtcttgg gaacttctac cagcgtttgt tcaataatca tgacccgggc ttacctttgc
     8041 tcgcttctac tgtaagcgac gacccgattc ctttatctga aaggtatgtt ctcccggaca
     8101 taaccgactc gcgctccatt gaaaccgagc agcccatcga tggtgccggt tatcacttag
     8161 gtctatccgg gctcaaggag cttcggtcgg gtgatactcc acgaggggaa ctgctgagtg
     8221 aaaagtccta tccatcgtac aaatatcaaa atgtatccca aaggcagcgt gtcgaaacct
     8281 ggctgggggc gaatcggcgc tgcgtcatcc tcggcggccc ggggagtgga aaaagctcac
     8341 tactacggtt tctatgtctc gatctttttc aggatgagcc gcagctgaca gaactggcac
     8401 gttggtgggg ccagttcctc cccgtctgga taccatttgc attttggaca aggaaattct
     8461 ccacgtccga gggtagagac gttccgctca cggcgatgct tgaggattgg ttcaagggtt
     8521 gggatgaagg tcacctctgg cccatcgtca ggcaggctct cgacgatgaa aggttactct
     8581 tgcttgttga cggtctcgac gagtatagcg atgaggacgc cgcgagaaat gctctcctgc
     8641 gactcaagat attcatcgaa cagcgaaacg caacggccgt cctcaccgcg cggccgcgag
     8701 gtttcgcacg cctaggcatg cagacggcgg ggtggggacg cggagacctg agtgatctct
     8761 ccgaggagca gcaggaacaa ctcacacgaa tctggttcac tcatcgggag cggaaggtcc
     8821 gcgggagtgc cggcgacgac gaagatgtta tcgcctgccg cgttgaagct gaagtgcaga
     8881 ggtttctggg tgatctccga agatcgagcg agcttcgtga gctcgctagg gtaccgctat
     8941 tgctctgcct tctgatcgct tacaagattc tccatgtaca actaccgcag aacaggttca
     9001 aagcttacca ggctttggtt gattacctga ttcatactca ccccgagagg agaaggctgg
     9061 cggcgctgat cagggagaca cctcttgcct taactgacga ggaagtgaaa agggcttttg
     9121 ccaacttggc ctaccgtatt cagtctcaat atgggacggg cgtcgtggag cagacggagg
     9181 cgatcaaagc tacgactcag ttcctcagtg acgagagtgg ggtaatcggt tttgaagcgg
     9241 cagaggcgag aaaagtaagc agggaattaa tccacaccgg cgaagacacc ctcgggcttc
     9301 ttgtcgaaag gtcgcaaagt gaaattggtt tttttcaccg cgcgctgcaa gagtatttag
     9361 ccgcgtatca cctatctacc atttcacata acgagcgagt gtctatagtt gaggagcatt
     9421 gtgtagagcc tcaatggcga gaggtaatac ttaacctctt ctccatctta aacagcatag
     9481 acgaggtaag tagttatgta agaagcgcct ttaaaaggca ggtagctccc cacgagcgtt
     9541 atgagatcga acagcttcta gctgaggtgg tcttcggcgg gttcaatgtc cccgtaggct
     9601 tatcgcgtga gatcgcgcgg actgtcttcc gggtggtaga gacggaatca tggatgccgc
     9661 accgagaacg gcttctcgac caagctgtcg ccggtttgcg ctcggccaag acgagggaga
     9721 tggttaagga caaactaaaa gtttggttcc cggacagaac tcgtaagatt cgtgcggaaa
     9781 tgttttccgc cgtggccgga tggcccagct ccgccgactc aaatcagtgt ttgctgctgg
     9841 ggctccggga cgaggactcc acaattaagc gcgcggcagc gaaggctttg gcggcgctta
     9901 atcaaggtga tgaggaaatc ggcgagcagg tggcagaact tgcatggagc gaagcagatc
     9961 cacggaccag agctgcagca atcgaatgtc taatgctcgg atggcccaat cactctcggt
    10021 taacctccat cattgatgca gctagggcgt ccgtcttccc acgcttacgt ctgatgggca
    10081 ttaatggaaa ggtacgacag ggtcgtgcta ctgacgagga tcttaatcag ttgttctacc
    10141 ttggctcacg ggacgcgggc gttgaccgca ggtggtttga cttcctcgtt gaatccttag
    10201 tcaaggggtg gacaggctcc ccagaagtca agcagagatg tcttgacgca gtcgaaagga
    10261 cgaagacggc ttttggggcg atgtctgatt ttggattata cggtgacttg gggaaggccg
    10321 tcctacttag cggttaccca caggataacg aagtcgcaca agttttagcc cgtgaagtga
    10381 actggggtag tgtcagcctc agggatgcaa acatgcgtag cgtcgacttc ttcgatctac
    10441 tagctaaaaa ctttaaagac cacccgaaag tagtggaggc agtagacagg tggttacggg
    10501 ctcaaaaggg agagaggaaa ggctcggggt tctaccgcgc tgctctagtc ggacgtacga
    10561 cgttagcaaa gtctctactc atcgactccc ttcattcaaa acatgcgggg ttagcggctc
    10621 agacattatt ggaagggtgg gggcttcagg accaagacgt agctcaagcc ctgttgagtg
    10681 cggctaacgg gcctcacgaa tgggcctctc aactaggaga atatctcccg ctgattatta
    10741 cagacgtaga catatgtaga agacggcttc ttgagctgat aagtcatgcc cctccctcgg
    10801 ccgtcgattc agttctaaag ggtttggcct ccttggggga gacctttgat gacgcggagg
    10861 tagtcgatgc cgtcctgcaa attccagtag ataattatgg acatcagccg ggacataaag
    10921 cactcattaa atactaccct tgggacagcc gtgttcggga actcgcaaag gatgtgctga
    10981 ataaccctga cgacagactc tcggcggtgg caaccgccta cggcgcggat gattatttta
    11041 gatcgcgaat tatagagctg atgactccct tgccggccca cttacgcaag gtgatggcgc
    11101 agagactcgg cgaggaggtg ggggacgact tcagccttac gctgctcgaa tcgtacaacg
    11161 acgaggcaga cgccgacgtg agggtacagg cgtcaattag ctatcacagt cttctgaaga
    11221 cgctaggccg ggacacagat gctgctatta aagtcctatc caatcggata gtggattaca
    11281 gtaaggattg ggcggcgaca cgccacgccg cgtttagtgg cctgcttgtg ttaggacggc
    11341 tggacgtgat gatgaacgcc caagagaaag ctcccaaact cgaagacata cgttgtcgct
    11401 tccctctatc aaggggggaa atttctccga gtgtgccctt gattcggtta ctgctcaaga
    11461 actgggtgta tatccgggca acgttcggtg aagagttctg gtttcgcctg gggtatgaag
    11521 aagaaagatt aaggagtgat ctatggtact acatcatccc gtttgcaaac gaatatcctc
    11581 agccccgcgc agaagttata aattacttgg aggcttctac agagaagagt cgtttttaca
    11641 gcccacgcag attgcatttc ctcagtcgcg ccttacccaa gagcacctta cttcgtcagt
    11701 actgtatgtc catccttgcc ggtgatgaga ataagtttga tttcgacggt gaagagtcag
    11761 taattgcggc agaaattata gccagccagt ttcgcgatga tgaggtcgtt caacggtggc
    11821 tgaatgaaaa tattcgtgaa gattacggcg aggaaggtgt gccagacaac ataattcttt
    11881 tgctatg
//
LOCUS       DASWNS010000553         8353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_331487, whole genome shotgun sequence.
ACCESSION   DASWNS010000553 DASWNS010000000
VERSION     DASWNS010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331487"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2955)
                     /locus_tag="VGB76_19110"
     CDS             complement(<1..2955)
                     /locus_tag="VGB76_19110"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEY0101042.1"
                     /translation="MDRLKVASLLALLLAVVIVVLPLGVSAALVVNDTFADGNSQNQD
                     LANSSLRVFKGRADTARVDAPGSVTFNVNTTSAEAFWAFFTNPGAPVSLNVGDKLSVA
                     GTFALSGFVGGDIRFGVFDSQGTRNTADLTGGMNNATFAGDPGYAVQFNTSGSGAPFG
                     LYRRANLSGNNPFNTLTDFTQISGSGATARQTLANDMSYTLTYTIERVSPADTRLTVS
                     VTGGTLPTGYSLTAVESSTSPVTAFDYFGFRFSGTAFARSIKFTNWRVDYSPSLPIIT
                     SQPQPSNLVVQVGSEVTMSVAASGNSLGYQWHKDGAPVAGNPSATTPTLNITNAQLAD
                     AGVYTAVVSNPGGSVASNPVTLGVSTEPVTPPPSIRAQPADTSAIVNTPASLSVTAEG
                     EGLFYQWFKNGALIPGATQPALAFASAQVGDSAAYTVVVSNSSGSVTSATARLLVVSA
                     MSAQSFAPAAGATDLNIDTPLRVTFDQTPLVGNSGRIRIFQEDGTLVDTIDLAADTQP
                     AVPGPQAQRNFGGASINQNYHPIIVTDNTASIYPRQSLAYNQTYYATLEPGVLTDASG
                     APFAGMQDPNVWRFSTKAAAPAAGNTALTVAADGTGDFDTVQGAVDFVPQNNSKRVVI
                     TVRKGAFTGIVYVRSNKPLITVRGEDREASVIRYANNNNLNPSTLGRAAFGVDAPDFT
                     LENITLVNTTPVGGSQAEAFRGNNQRITLNRVNLKSFQDTLLLQGAGFVNDSYIEGDV
                     DFMWGSGAVFFQNSELKMVRSNAYYTQIRNGSGVNGNVYVNCRLTAAPGVTGAYLTRI
                     DPDDFPFSQVVFINTVMDAHVRPDAWRFDNPLNAVTAANYPNIRFWEYNTRDLNGNPA
                     DVSQRHPISRQLTAAEAIQWSNPSFVLGGWTPQTTLTAGVNLSNLNQGYTGSPVVPTV
                     ITDPPGLNVSLTYNGSPVPPTAPGSYTLLATVNDANYQGSASGTLLVNPVSASITFGN
                     LYQVYDGAPKSVN"
     gene            3165..4295
                     /locus_tag="VGB76_19115"
     CDS             3165..4295
                     /locus_tag="VGB76_19115"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEY0101043.1"
                     /translation="MRELKKHALRNVARENVTSMTSSLVLWVSGFYGVSLTPAAFPVM
                     SMMNFLTRRRLRLWCLLSVITFGFANVAAGQTELVVATDGSAKYKTVQEAIMAVPAGS
                     PANPVVIRIKPGRYRELIYVQREKRFFRLIGEDAARTIITHDLHANLTGHDGKPIGTF
                     RTPTATIDADDFIAENLTFENSAGAVGQALALRVDGDRAVFRNCRFLGWQDTIFLNRG
                     RQYFEDCYIAGHVDFIFGAATAFFERCHIHSLRDGYITAASTPFDQPFGFVFSNCRIT
                     GETPAIRTYLGRPWRAHSSVTFLNTEMSGVVHAEGWHNWNLPEREKTVRYAEWGSRGA
                     GANPRARVQWSRQLKRAEAKAFTPEKVLGGADGWKPGKSRKS"
     gene            complement(4364..6037)
                     /locus_tag="VGB76_19120"
     CDS             complement(4364..6037)
                     /locus_tag="VGB76_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HEY0101044.1"
                     /translation="MNLKSLLVRIVGLLLALMPLSYVSWAQTSAPSNPAATPVSPVSK
                     VWVADNGDGTYRNPIIHADYSDPDAVRVGDDFYMTASSFNAAPGLPILHSKDLVNWRL
                     VGHVFTRQRPLDVFSKPQHGNGVWAPSIRHHNGEFYIYYPDPDHGIYLTKAKHPAGQW
                     SEPLLVKEAKGWIDPCPLWDTDGNAYLVSALAASRSGVKSILVVSRMSADGTRLLDDG
                     VMVFDGHDRHPTLEGPKFYKRNGYYYIFAPAGGVEQGWQLVLRSKNVYGPYEEKIVLA
                     QGKSLVNGPHQGAWVETQAGESWFIHFQDKGAYGRIVHLQPVKWANDWPVIGADADAD
                     GTGEPVLSHRKPNVGASWPVVTPPDSDEFDAARLGLQWQWHANPGTHWAFPAPALGFL
                     RLFNVPLPESYKNLWDVPNLLLQKFPAEEFTATTRTTFTPRTDEEETGLVLMGLDYAH
                     ISVKKKPAGLFVSQTVVKDAEGGGKGQESAPMLLKGNTFYLRVKVSKEAACQFSYSTD
                     GSNFMPVGLPFVARKGKWIGAKVGLYAIRTGRTRETGYADFDWFRIEKP"
     gene            complement(6187..6948)
                     /gene="kduD"
                     /locus_tag="VGB76_19125"
     CDS             complement(6187..6948)
                     /gene="kduD"
                     /locus_tag="VGB76_19125"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HEY0101045.1"
                     /translation="MILDKFRLDGKVALVTGASAGLGAAIALALAEAGADIAAHGNSR
                     PPGATCELIRNAGRRGLALRGDLADRETPRRLVSGVLEEFGRLDILVNNAGTIRRAPA
                     AEYSEEDWAAVIEVNLSSVFRLSQLAGRHMLEHGGGKIVNIASLLSFQGGVTVPAYAA
                     SKGGVAQLTKALANEWAARGINVNAVAPGYMRTDNTAALQRDEVRNRQILERIPAGRW
                     GEPEDLSGAVVFLCSAASDYLHGHVLAVDGGWLGR"
     gene            complement(6945..7367)
                     /locus_tag="VGB76_19130"
     CDS             complement(6945..7367)
                     /locus_tag="VGB76_19130"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0101046.1"
                     /translation="MSATQPDETRPLAGSAQAARPLHLNWSEVPVEQVGEGIERQMVV
                     GEKMMVCRLRFAPFVVTPEHEHTHEQMTIVERGRVRFFIEGEERIASPGDVLHFPSGC
                     WHGATMLDEEVVLVDIFTPLREDFLSNQGDVRESGRAK"
     gene            complement(7383..8222)
                     /locus_tag="VGB76_19135"
     CDS             complement(7383..8222)
                     /locus_tag="VGB76_19135"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEY0101047.1"
                     /translation="MQAEATQTEITKERCYVPATHAGRGRRTAVAPGQTAARYLHYGR
                     ITLDAGDETLAYENGTHETGLVCLNGAAEISTGGQTFTLGRYDALYVPRDSRIEVRPA
                     GEAGCDLAELSAPVEGSYPLQFVSFAEVRENPKLHIIAGAPPAERDMNVLIGSNVRAG
                     RIMTGVTFSRPGNWTSWPPHEHSRMLEEAYLYIDMPAPSFGVQFVYTNPEEPELVQVV
                     REGDVVLMPKGYHPNVAAPGGQINFMWMMAAMREGEDRKYGVVNVQPEYAAGGSGLEA
                     VKD"
BASE COUNT         1491 a         2482 c         2756 g         1624 t
ORIGIN      
        1 gttcacgctc ttcggcgcgc cgtcgtaaac ctgatagaga ttgccgaatg tgatcgaggc
       61 cgacacgggg ttgacgagga gtgtgcccga ggcagagcct tgatagttgg catcattaac
      121 ggtcgctaag agagtgtaag agccgggggc tgtgggcgga acggggctgc cgttgtaagt
      181 gagagagacg ttcaatcccg gcggatcggt gatcaccgtc gggacgacgg gcgagcctgt
      241 gtagccctga ttcaggttgg agaggttgac gcccgccgtg agcgtggttt gcggcgtcca
      301 accgccgagc acgaacgagg gattgctcca ctggatggcc tccgcagccg tgagttggcg
      361 cgagatgggg tggcgttggc tcacgtccgc cggattgccg ttaaggtcgc gggtgttgta
      421 ctcccagaag cggatattcg ggtagttggc ggcggtcacg gcgttcagcg ggttgtcgaa
      481 acgccatgcg tcggggcgga cgtgcgcgtc catcacggtg ttgatgaaga cgacctgact
      541 gaaaggaaag tcgtccgggt caatgcgcgt caggtacgcg ccggtgacgc cgggcgcagc
      601 cgtcaggcgg cagttgacat acacgttgcc gttgacgcct gagccgttgc gtatctgcgt
      661 gtagtaggcg tttgacctga ccatcttcag ttcgctgttc tggaagaaga ccgcgccgct
      721 gccccacatg aagtcaacgt cgccttcgat gtaactgtcg ttgacgaagc ccgcgccctg
      781 caagagcagg gtgtcctgaa agctcttgag gttgacgcgg ttgagcgtga tgcgctggtt
      841 gttgccgcga aacgcctcgg cctgcgagcc gcccacgggc gtcgtgttga cgagggtgat
      901 gttttccagc gtgaagtcag gcgcgtctac gccgaaggcc gcgcgcccca gtgtgctcgg
      961 attgaggttg ttgttgttgg cgtagcggat aacgctcgcc tcacggtctt caccgcgcac
     1021 cgtgatgaga ggcttgttcg agcgcacgta gacgatgccc gtgaatgcgc ccttgcggac
     1081 ggtgataacc acacgcttgc tgttgttctg aggtacaaaa tcgactgcgc cctgcaccgt
     1141 gtcgaagtcg cccgtgccat cggcggccac agtcagcgcg gtgttgcctg cggcgggggc
     1201 ggcggccttc gtcgagaaac gccacacgtt cgggtcttgc attccggcga agggcgcgcc
     1261 gctcgcgtcc gtcaggacgc ccggctccag agtggcgtag taggtctggt tgtaggcgag
     1321 cgactggcgc gggtagatcg aggccgtgtt gtccgtcacg atgatcgggt gatagttctg
     1381 gttgatagac gcgccgccga agttgcgctg cgcctgtggt ccgggaacag ccggctgcgt
     1441 gtctgcggcc agatcgatgg tgtcaacgag cgtgccgtct tcctgaaaga tgcgtatgcg
     1501 gccagagttc ccaaccagcg gcgtctggtc gaaggtgacg cgcagaggcg tgtcgatgtt
     1561 gaggtcggtg gctcccgccg cgggcgcgaa actctgggca gacatcgcag agacgacgag
     1621 caggcgagcg gtcgcgctag taacgctccc gctggagttg ctgacgacaa ccgtgtaagc
     1681 cgccgaatcg ccgacctgcg cggaggcgaa ggccaacgcg ggctgggtcg cgcccgggat
     1741 gagcgcgccg tttttgaacc actggtagaa aagcccttca ccttccgccg taacggagag
     1801 gctcgcgggg gtgttgacga tggcagacgt atcggctggc tgcgccctga tgctcggcgg
     1861 cggcgtgaca ggctcggtgc tgacgccaag cgtcacggga ttgctcgcca cgctgccgcc
     1921 gggattgctg acgacagcgg tgtagacgcc cgcgtccgcg agttgcgcgt ttgtgatatt
     1981 aagcgtaggg gtcgtggccg aagggttgcc agcgacgggc gcgccgtcct tgtgccactg
     2041 gtagcccaga gagttgccgc tcgccgcgac cgacatggtc acctcgctgc caacctgcac
     2101 gacgaggttc gagggttgcg gctggctcgt gatgatcggc agcgaagggc tatagtccac
     2161 gcgccagttc gtaaacttga ttgaacgcgc gaaggctgtg ccgctgaaac gaaagccgaa
     2221 atagtcgaag gctgtgacgg gcgacgtgct cgactcaacc gccgttaaag aatagcctgt
     2281 ggggagcgtg ccgcccgtga cggagactgt gaggcgcgtg tcggctggcg acacgcgctc
     2341 gatggtgtag gtgagcgtgt aggacatatc gttggcgagc gtctgcctcg ccgtagctcc
     2401 cgacccggag atttgcgtga agtccgtgag cgtgttgaag gggttgttgc cggagagatt
     2461 cgcacggcga tagaggccga agggcgcgcc gctcccgctc gtgttgaact ggacggcgta
     2521 gccggggtcg cccgcgaagg tcgcgttatt catgccgccc gtcaggtcgg cagtgttgcg
     2581 cgtgccctgt gaatcgaaga cgccgaagcg gatgtctccg ccgacgaaac ccgagagggc
     2641 aaaagttccc gcgaccgata atttgtctcc cacgttgagg ctgacaggcg cgccggggtt
     2701 ggtgaagaac gcccagaagg cttccgcgct cgtcgtgttg acgttgaagg tcacagaccc
     2761 cggggcgtct acccgcgccg tgtccgcgcg ccccttaaag accctgaggg aactgttcgc
     2821 caagtcctga ttctggctgt tgccgtcggc gaatgtatcg ttgacgacca aggcggcact
     2881 gacaccgagc ggtaaaacca ctatgacgac tgcaagcaag agggcgagta gcgaggcgac
     2941 cttcaatcta tccataatcg tttatagctc cactaatagc gagaatgact tacacgcttg
     3001 aaaagatgat caatgtttgg aggaggatgc gggttgattg gtcattcaac tattgtgtat
     3061 attactaatt ggtttgacca acaaggcggg ttttatacca tgcctgcgat tttgggtcaa
     3121 gccttttcaa gacggctgtc cgcgcatgag aactgtctga cagtgtgcga gagttgaaaa
     3181 agcacgcgct gcgaaacgtt gcgcgggaga atgtgacgag catgacgagt tcgctcgtgc
     3241 tgtgggtgtc aggcttctac ggcgtcagct taacgcctgc cgctttccct gttatgagta
     3301 tgatgaactt cttgacgcga agacgtttga gactctggtg cctgctgtcc gtcatcactt
     3361 tcggtttcgc caacgtcgcg gcggggcaga ccgaactggt cgttgcgacg gacggaagcg
     3421 cgaagtataa gacggtgcag gaggccatca tggcggtgcc tgcgggcagc cctgcgaacc
     3481 cggtcgtgat tcgcatcaag cctggccgct acagggaact gatctacgta cagcgcgaga
     3541 aacgcttctt ccgcctcatc ggagaggacg ccgcacggac gatcatcaca cacgacctcc
     3601 acgccaacct cacaggtcac gacggcaagc ccatcggcac cttccgcacg cccacggcca
     3661 ccatcgacgc cgacgacttc atagccgaga atctcacctt cgaaaactcc gcaggggcag
     3721 tcgggcaggc gctcgccctt cgcgtggacg gcgaccgcgc agtcttccgc aactgccgct
     3781 tcctcggctg gcaagacacg atcttcctca atcgcgggcg acagtatttc gaggactgtt
     3841 acatcgcggg gcacgtagac ttcatcttcg gcgcggcgac cgccttcttc gagcgttgtc
     3901 acatacactc cctgcgcgac ggttacatca cagcggcctc gactcccttc gaccagccgt
     3961 tcggctttgt cttctcgaac tgccgcatca ccggcgagac gcccgccatc aggacttacc
     4021 tcggtcgccc ctggcgcgcg cattcgagcg tgacctttct gaacacagag atgtcggggg
     4081 tggtacacgc ggagggctgg cacaactgga acttacccga gcgcgagaag actgtgcgtt
     4141 acgccgaatg gggaagtcgc ggcgcgggcg cgaacccgcg cgctcgtgtc caatggtcgc
     4201 gtcaactcaa gcgcgccgaa gcgaaagcct tcacgccgga aaaagtcctc ggcggcgcgg
     4261 acggctggaa gcccggaaaa agccgtaaat cgtgaatcgt gaatcgtaag tcgtgaatag
     4321 agaaaacctg atctacttct attcacgatt tacgattcac gattcacggc ttttcgattc
     4381 tgaaccaatc gaagtctgcg taaccggttt ctcgcgtcct gcccgtgcgg atagcgtaga
     4441 ggccgacctt ggctccgatc cacttgcctt tgcgcgcaac gaaaggaagg ccgacgggca
     4501 tgaagttgga gccgtctgtg ctgtagctga actggcaggc ggcctccttc gacaccttca
     4561 cgcgtaggta aaacgtgttt cccttcaaga gcatcggcgc gctctcctgc cctttaccgc
     4621 ccccttcggc atccttcacc acggtctgtg agacgaacag cccggcgggc ttcttcttca
     4681 cggagatgtg tgcgtagtcg agtcccatca agacgagtcc cgtctcctct tcgtcggtgc
     4741 gcggcgtgaa ggtggttctg gtggtggccg taaactcttc ggcggggaac ttctgcaaga
     4801 gcaggttggg cacgtcccag aggtttttgt acgactccgg gaggggcacg ttgaagaggc
     4861 gtaggaaacc gagcgcaggg gcggggaacg cccagtgtgt gccggggttg gcatgccatt
     4921 gccactggag gccgagacgc gcggcgtcaa actcgtcaga gtcgggcggc gtcacgacag
     4981 gccacgacgc gcccacgttc ggcttccggt gagagaggac aggctcgcct gtgccgtccg
     5041 cgtcggcgtc cgctccgatg accggccagt cgttcgccca cttcacaggt tgaaggtgga
     5101 cgatacgacc gtatgcgccc ttgtcctgaa agtgtatgaa ccatgactcg cctgcctgcg
     5161 tctcgaccca tgcgccctga tgtggaccgt taaccagact cttgccctgc gcgagcacga
     5221 tcttctcttc gtagggaccg tagacgttct tcgagcggag cacgagttgc caaccctgct
     5281 ccacgccgcc cgccggtgcg aagatgtagt agtagccgtt ccgtttataa aactttggtc
     5341 cttcgagcgt cgggtgcctg tcgtgcccgt cgaagaccat gacgccatcg tcgagaaggc
     5401 gcgtcccgtc cgcgctcatg cggctgacga cgaggatgct cttgacgccg gagcggctcg
     5461 cggccaatgc gctcacgaga taggcgttgc cgtctgtatc ccagagaggg cacgggtcga
     5521 tccagccctt cgcttccttg acgagcagcg gctcagacca ttgcccggca gggtgtttcg
     5581 ccttcgtcag gtagatgccg tggtctgggt ctgggtagta gatgtagaac tcgccgttgt
     5641 gatgacggat ggacggcgcc cagacgccgt tgccgtgctg cggtttgctg aacacgtcga
     5701 gaggtcgctg gcgtgtgaag acgtgtccga cgagccgcca gttgacgagg tctttggaat
     5761 ggaggatggg cagccccggc gcggcgttga agctcgacgc ggtcatgtag aagtcgtcgc
     5821 cgacacggac ggcgtcaggg tcggagtagt cggcgtggat gatggggttc ctgtacgtcc
     5881 cgtcgccgtt gtccgccacc cagaccttcg acacaggcga cacaggcgtg gcggcggggt
     5941 tcgaaggcgc ggaggtttgc gcccacgaca cgtacgagag gggcatgagc gcaaggagca
     6001 atccgacgat tcgcacgagg agacttttca ggttcatgtg tgcgctagat taagtaaaga
     6061 atagtttgcg agacggcaga ccggccacga gggcacggag agacgggttt gcatcgagag
     6121 agttgcgccg caggtttctg ctccacagtg tctctgcgat gaacttgaga gcgacgcgcg
     6181 ctcaggtcac ctgccgagcc agccgccgtc aacggcgaga acgtggccgt gcaggtaatc
     6241 gctcgccgcc gagcagagga agacgacagc gccggacaaa tcttccggct cgccccaacg
     6301 tccggcgggg atgcgttcga gaatttgccg gttccgcact tcgtcccgtt gcaacgctgc
     6361 ggtgttgtcg gtgcgcatgt aaccgggcgc gacggcgttg acgttgatcc ctctcgccgc
     6421 ccactcgttg gcgagagcct tcgtcaattg cgccaccccg cccttcgacg ccgcataggc
     6481 cgggacggtg acgccgccct gaaacgagag caggctggcg atgttgacga tcttgccgcc
     6541 gccgtgttcg agcatgtggc ggcctgcgag ttgggagaga cgaaagacgc tcgacaggtt
     6601 gacctcgatg actgccgccc agtcttcctc cgagtactct gcggcaggcg cgcggcgaat
     6661 cgtgcccgcg ttgttgacga ggatgtccag acgcccgaac tcttcgagca cgccggagac
     6721 gaggcggcgc ggcgtctccc tgtcagcgag gtcgccgcgc agcgcgaggc cgcgccgacc
     6781 cgcgttcctg atgagttcgc aggtcgcacc gggcgggcgc gagttgccgt gcgcggctat
     6841 gtctgcgccc gcctctgcga gcgcgagcgc gatggccgcg ccgagtcctg ccgatgcgcc
     6901 cgtcacgagc gcgaccttgc cgtcgagcct gaacttgtcg aggatcattt cgcccgccca
     6961 ctctcgcgca cgtcgccctg attcgagagg aagtcctcgc gcagcggcgt gaagatgtca
     7021 acgaggacga cctcttcatc cagcatcgtc gcgccgtgcc agcagccgga agggaaatgg
     7081 agcacgtcgc ccggcgaggc gatgcgctcc tcgccctcta tgaaaaaacg gacgcgcccg
     7141 cgctcaacga tggtcatctg ctcgtgcgtg tgttcgtgct ccggcgtgac gacgaacggc
     7201 gcgaaacgca gacggcagac catcatcttc tcgccgacga ccatctgccg ctcgatgcct
     7261 tcgccgacct gctcgaccgg aacctccgac cagttcaggt ggagcggtcg cgcggcctgc
     7321 gcgctgcctg ccaacggacg tgtctcgtct ggttgtgtcg ccgacatctt ccaagtcctt
     7381 ttctagtcct tcaccgcttc gaggccggag ccaccggcgg catactccgg ctgcacgttg
     7441 acgacgccgt atttccggtc ttcgccctca cgcatggccg ccatcatcca catgaagttg
     7501 atctggccgc cgggcgcggc gacgttcggg tggtagccct tcggcatcaa caccacgtcg
     7561 ccttcacgca cgacctgcac cagttccggc tcttccgggt tcgtgtagac gaactgcacg
     7621 ccgaaggagg gcgcgggcat gtctatgtag agataagcct cttcgagcat ccgcgagtgc
     7681 tcgtgcggcg gccacgatgt ccagttgccg gggcgcgaga aggtcacgcc ggtcatgatg
     7741 cgtccggcgc ggacgttgct gccgatgagc acgttcatgt cgcgctcggc gggaggcgcg
     7801 cccgcgatga tgtgcagttt cgggttctcg cgcacctccg cgaaggagac gaactgtagc
     7861 ggataactgc cctcgacggg tgcggagagt tccgccagat cgcaaccggc ttcgcccgca
     7921 gggcgaactt cgatgcgcga atcacgcggg acgtagagcg cgtcgtagcg tccgagcgtg
     7981 aacgtctggc cgccggtcga aatttcggct gcgccgttca ggcagacgag tccggtttcg
     8041 tgcgtgccgt tctcgtaggc gagcgtttcg tcgcccgcgt cgagcgtgat gcgcccgtag
     8101 tgtaggtaac gcgcagccgt ctggccgggg gcaaccgccg tgcgccgtcc ccgcccggcg
     8161 tgggtcgcag gcacgtagca tctctctttc gtgatctctg tctgtgtcgc ttcagcttgc
     8221 attagtcaac ctccctgtgt gaaaacgatg aacgcggaac gatgaatgat ggacggaaga
     8281 aggctcgcgc ctgttgtgtc gcagcgaaga tatgacttcg cggcggcatc ctccgccctg
     8341 cactcgcaac ctt
//
LOCUS       DASWNS010000554         8689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_383849, whole genome shotgun sequence.
ACCESSION   DASWNS010000554 DASWNS010000000
VERSION     DASWNS010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383849"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VGB76_19140"
     CDS             complement(<1..828)
                     /locus_tag="VGB76_19140"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEY0101048.1"
                     /translation="MKLSYGTARRSRGIAVLLSLIFLCNGVALAQRKAGTRRTTPGRP
                     TVAEAQEFVAHAEQRLTALSLKVSRASWIQENFITDDTEALAADANNELTAAVTQLAE
                     QATRFDNLKLPYDTARKLQLLKLALTVPAPSNPAERDELTKITASLSSDYGKGKYCPE
                     GDKGKCLTLQEMEPILAESRDPEELKRIWLGWHAISPPFKQKYARFVVLGNKGAREMG
                     FKDLGALWRSNYDMKPDEFAAEMERLWLQVKPLYDSLYTYTRWKLSEKYGKQLVPENQ
                     "
     gene            1121..2245
                     /locus_tag="VGB76_19145"
     CDS             1121..2245
                     /locus_tag="VGB76_19145"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0101049.1"
                     /translation="MKRTPLFPPPSHLRAALALSLFFLLAPAAFRIHAQQNASLSSEK
                     IKLVEAAIAAEMSRQNIPGLSVAIVTDNQLRWSNGYGFADLENFVPAKAGTVYRLASI
                     SKAVTAVAVMQLVERGKLDLDAPVQKYCPAFPEKQWPVTSRHILAHLSGIRHYKDEEE
                     YGSTRRYDTISAALDMFKNEPLLHEPGVKYTYTTFGYSVLGCALEGASGQKFADYLRD
                     NLFQPAAMDRMRVDSINELIPNRAQGYRRTRDGHLRNSDIADNSYKIAGGGLVSTVED
                     LAKFAIAVQANSVLKRETVEQMLTSQKLRNGKETNYGLGWSVQMQNNQKAVGHSGAQQ
                     RVSTALHLIPARNFAVALMVNLEDTRLRPLTDRITEIVLK"
     gene            complement(2285..2848)
                     /locus_tag="VGB76_19150"
     CDS             complement(2285..2848)
                     /locus_tag="VGB76_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101050.1"
                     /translation="MGDWKARTKHDLMIEVWERLDCETVGASELEAIAEAVRGRFGEG
                     AEESPAIVARLLADEGAELRHAEVLEADARWRATDPYEAMFRNVLKFSTFEEAAASIK
                     RLDNLRRQFGRKKDREGLRRVQETVLKGKQRAQMIARNPSVNERKRAEKEEIAEWFTV
                     WLRQPEIFEDWLHLRRRTKDFRARFGD"
     gene            3046..5820
                     /locus_tag="VGB76_19155"
     CDS             3046..5820
                     /locus_tag="VGB76_19155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEY0101051.1"
                     /translation="MSNLDSALAALREHFNFETFRAGQREVIEAILDGHDTVVVMPTG
                     GGKSLCFQLPALMKEGATIVVSPLIALMKDQVDALHARSLPATYINSSVDFEEQKARI
                     AGIRQGRYKLVYVAPERFRSAHFVEALKSVNVSLFAVDEAHCVSTWGHDFRPDFLRLK
                     AAIEEIGRPQVVALTATATPHVRADIIEQLGLREPRAYVSGFDRPNLSIKVVHTLKER
                     EKIAHVKSLAAAAQGGSGIIYSSTRKSVEQVARRLKDAGLSVVAYHAGMEEAERTRAQ
                     DDFMSGRVQMIVATNAFGMGIDKADIRFVIHYHLPGSIEAYYQEIGRAGRDGQPATCV
                     LLFNYADKRTQDYFIEGSYPPPEIIAKVYEALVATRLQHIELSTHEIAVRASVRNEMA
                     VQSALIILEKAGHIERGAAGENRAAVRLLMPPHLARETVNAKRSARDKQVLFTLLGGY
                     DLNERADSEIDVNEFAETAGLDLASVRRSLSSLAAAKVLSYTTARRTRGLQMLDERPV
                     TRLRIRPQDLARRAALEQRKLREMISFCYADRCYRAFILDYFGDRSHKPTCGTCGVCL
                     REKPAAANDAPALEAPDDYVESSFEDEVSPFSSTRDASDIPPVSPPPSSPRTRLDKFV
                     IENTPFASDLENELDRQSLAEHRRREAESFGAELNSSVQITGARPLGHDETLCVRKIL
                     ACVARMEGRFGKGILAACLRGSRSAKISQFGLERLSTYGILSNMTQDEILLYIDALVA
                     AGALRVAGGNYPTVAITSFGNDVMRERATIELALPSVAPVAAPPASFAMRGSRDAAVA
                     NVKAVNAPRVSTIDETYAFYKAGMTIEEISRERSIAEMTVEKHLADCILEGRPFDISR
                     HVNAVDRALIEEAVRRLGDERLKPLRDALPRHISYRMIRFVVADMQRAAAIASNGDAE
                     RVRTSEATGV"
     gene            5907..6122
                     /locus_tag="VGB76_19160"
     CDS             5907..6122
                     /locus_tag="VGB76_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101052.1"
                     /translation="MSRNPNTDPPQPEQQPELPTPQPHDPLRPDPSQPEPLPLPPGGE
                     PKSPIGEPDATPPPAGDPPVSEPTRLV"
     gene            6143..7255
                     /locus_tag="VGB76_19165"
     CDS             6143..7255
                     /locus_tag="VGB76_19165"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0101053.1"
                     /translation="METVQEEAAHTNGTGSVSGTAAHDDARLEEIASALSAKSFTPHP
                     LFRSGHAQTLVAYAWPRRGRLRALQTDEERLFEVEPGVRVLVRCRWHAERRAHPTLLL
                     VHGLEGSSESIYMLSTALKAHRAGFNVLRLNIRTCGNTLHLTPTLYNSGMSPDLRALI
                     SELVGRDKLKDIFLGGFSLGGNMSLKLAGEYGADFPRELKGVAVVSPSVELAASATCI
                     EQRANWIYQRKFIRSLKRRMRAAARLYPERYDASGLRRVRTLREFDERYTAPHGGFRD
                     ASDYYARSSSLPLISRIRVPTLIIHAQDDPFIPFASLRHPSVRANSSIILLAPAHGGH
                     VGFVGDGQTAGEDRFWAENRLVEFCRRINEGMRDEG"
     gene            7437..>8689
                     /locus_tag="VGB76_19170"
     CDS             7437..>8689
                     /locus_tag="VGB76_19170"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0101054.1"
                     /translation="MITALKLIATVLIVAINGFFVAAEFALVGVRRSRIETLAEDGDR
                     RAKRLLGILDNLNAYLSASQLGITLASLALAILGEPTIAHLLEAPLSRLNIGDTWRHA
                     IAFVIAYAILTSLHIVLGEQAPKLVGLERAEKVAMWSALPMELFYKFFSWPIRALDWA
                     SAAVVRPFGMNATSAHASIYTEEEIRQLINASHKGGHLEIEEQQLINRVFDFSEAEVR
                     EAMIPRTAVAAIPVTATLAEVEESFCETGYSRLPVYRDQLDNIIGVLFMKDIMPCLKT
                     SGRTEFDMEKMVHPPMFVPATARLGSVLAQMQAARTHLAFAIDEHGGIEGILTLEDLL
                     EEIVGEINDEYDEEVRAQIIEENGAYVLDGMLAVRDANRKFKLKLPEDGGYTTLAGFL
                     LARAGRILKQGETVEHDGASFMVERV"
BASE COUNT         1695 a         2958 c         2443 g         1593 t
ORIGIN      
        1 ctggttctcc ggcacgagtt gcttaccgta cttctccgag agcttccagc gcgtgtatgt
       61 gtagagcgaa tcgtagagcg gtttgacttg cagccagagc cgctccatct cggcggcaaa
      121 ctcgtccggc ttcatgtcgt agttggaacg ccacaacgcg cccaaatctt tgaagcccat
      181 ctcgcgcgcg cccttgttgc cgagcacgac gaagcgcgca tacttctgtt tgaagggcgg
      241 cgagatggcg tgccagccga gccagatgcg tttcaactct tccgggtcgc ggctctccgc
      301 gaggatgggt tccatctctt ggagcgtcag gcattttccc ttgtcgcctt cggggcaata
      361 cttccctttg ccgtaatcgc tcgacagcga ggccgtgatc ttcgtgagtt cgtcgcgctc
      421 cgccgggttg gaaggcgcgg gcacggtcag ggcgagtttc aacaactgga gttttcgcgc
      481 cgtgtcgtaa ggcagcttca aattgtcgaa ccgcgtcgcc tgttcggcca actgcgtgac
      541 ggcggcggtc aattcgttgt tcgcgtcggc ggccagagcc tccgtgtcgt ccgtgatgaa
      601 gttctcctga atccaactcg cacgactcac tttaagactg agtgcggtga gccgctgctc
      661 cgcgtgcgcg acaaactcct gcgcctccgc gacggtcggc ctgcccggcg tcgtccgccg
      721 cgtcccggcc ttacgctgcg ccaacgccac accgttgcag agaaaaatca atgacagcag
      781 cacggcgatg ccacgcgaac gccgcgccgt cccgtaggac agtttcatac actacctcca
      841 gcttgagtaa aatcggtttg tgcttcgaca tgtaaaattt caggggagga gtcgccctta
      901 ttttaacttc aaacgcagcc cgtcacatag cgcgggcggg ggtgcccgcc gcagagaagc
      961 gacgcgacgt gccggaagca gagagccgcc gggcagcaag gttgtccgaa caaaattccc
     1021 cgcgcccgtt aacaaaagcg tccgccctca cgtatgtaga taatgcaagc ctcgcatatc
     1081 ctgtccggca gcctaatctt ctatcaggag aagacatcga atgaaacgaa cgcccctctt
     1141 ccctccgccg tcgcatctgc gtgctgcgct cgctttgagc ctgttctttc tacttgcgcc
     1201 cgccgccttc cgtatccacg cgcaacagaa cgcgtcgctc tcatcggaga agatcaaact
     1261 ggtcgaagcc gccatcgccg ccgagatgtc cagacagaac attcccggcc tctccgtcgc
     1321 catcgtcacc gataatcaac tgcgctggtc gaacggttac gggttcgccg acctcgaaaa
     1381 cttcgtcccg gccaaagcgg gcacggtcta tcgtctggct tcgatctcta aagccgtcac
     1441 cgccgtcgcc gtcatgcaac tcgtcgagcg cggcaaactc gacctcgacg cgcccgtgca
     1501 aaaatattgc cccgcctttc ccgaaaaaca atggcctgtc acctcgcgcc acatactcgc
     1561 ccacctgagc ggcattcgcc attacaagga cgaagaagag tacggcagca cccgccgcta
     1621 cgacaccatc tccgccgcac tcgacatgtt caagaacgag ccgctgctcc acgagccggg
     1681 cgtgaaatat acctatacaa ccttcggcta cagcgtgctc ggctgcgccc tcgaaggcgc
     1741 gtccggccag aagttcgcag actacctgcg cgacaacctg ttccagcccg ccgcgatgga
     1801 tcgcatgcgc gttgacagca tcaacgaact catccccaac cgcgcgcaag gctaccggcg
     1861 cacgcgcgac ggccacttgc gcaactccga catcgccgac aacagttaca aaatagcggg
     1921 cggcggcctc gtctcaaccg tggaagacct ggcgaagttc gccatcgccg tgcaggccaa
     1981 ctccgtgctt aaacgagaga cggtcgagca gatgctcacg agccagaagt tgcgcaacgg
     2041 gaaggagacg aactacgggc ttgggtggag cgtccagatg cagaacaatc aaaaggcggt
     2101 cggccactcg ggcgcgcagc aacgcgtgag cacagccctc cacctgatcc ccgcacgcaa
     2161 tttcgcagtc gccctgatgg tgaacctcga agacacgcgc ctccgcccgc tcacagaccg
     2221 gatcacggag atcgtcttga agtagttgga agaatttttg cgacgactgc ggcgggcgca
     2281 tctctcaatc gccaaaacgc gcccggaaat ctttcgtccg gcggcggagg tgaagccagt
     2341 cttcgaagat ctccggttgc ctgagccaga ccgtgaacca ctccgcgatc tcttccttct
     2401 ccgcgcgctt ccgctcgttc accgaaggat tgcgcgcgat catctgcgcc cgctgcttgc
     2461 ccttcaacac cgtctcctgc acgcggcgca atccctctct gtctttcttg cgcccgaact
     2521 ggcggcgcag attatccaga cgcttgatcg aggcggcggc ctcttcgaag gtcgaaaact
     2581 tcaggacgtt gcgaaacatc gcctcgtaag gatcggttgc gcgccagcgc gcgtccgcct
     2641 ccaacacttc ggcgtggcgc aactctgcgc cctcgtccgc gagcagccgc gccactatcg
     2701 cgggactctc ctccgcgcct tcgccaaacc gcccgcgcac cgcctctgcg atggcttcca
     2761 actcgctcgc gcccaccgtc tcgcaatcca gacgctccca cacctcgatc atcaggtcgt
     2821 gttttgtacg cgctttccag tcgcccattc gataaagggt aaagggtaaa aggtaaaggg
     2881 taaagggcaa atggcgaggt gatgtttccc tcccgccttc gcccttcccc gccagccctt
     2941 ccccgccagc cctcgcctgc gccgctcgcc attaaccgtc tccctccgct ttgcggtaaa
     3001 atctaccctt gtctcgcgct cgttgcccgt gagactcaac ctgtgatgtc aaatctcgat
     3061 tcagccctcg ccgccctgcg cgaacatttc aactttgaga ctttccgcgc cggacagcgg
     3121 gaagttatcg aagccatcct cgacggacac gacacggtcg tcgtgatgcc gacgggcggc
     3181 ggcaagtcgc tctgtttcca gcttcccgcg ctgatgaaag agggcgcaac catcgtcgtc
     3241 tcgccgctca tcgccctgat gaaagatcag gttgacgcgc tgcacgcgcg ctcgctcccc
     3301 gccacttaca tcaacagttc cgtggacttt gaggaacaga aggcgcgcat cgcgggcatc
     3361 cgtcagggac gctacaagct cgtctatgtc gcccctgaac gcttccgcag cgcgcatttc
     3421 gtggaagctc tcaagagcgt caacgtctcg ctcttcgccg tggacgaagc gcactgcgtc
     3481 tcgacgtggg gacacgactt tcgccccgac tttctccggc tcaaagcggc catcgaagag
     3541 atcgggcgac cgcaggttgt cgcgctcacc gcgacggcca cgccgcacgt tcgcgcagac
     3601 atcatcgaac aactcggctt gcgtgagccg cgcgcctacg tctccggctt cgaccgcccg
     3661 aacctctcca tcaaagtcgt tcacacgctc aaagaacgcg aaaagatcgc gcacgtcaaa
     3721 tcgctcgccg cggcggcgca gggcggctcc gggatcatct attcttccac gcgcaagtcg
     3781 gtcgaacagg tggcgcggcg tctgaaagat gcgggcttga gcgtcgtcgc ctatcacgcg
     3841 gggatggagg aagcggagcg cacgcgtgcg caggacgatt tcatgtccgg gcgcgtgcag
     3901 atgatcgtgg cgacgaacgc cttcggcatg ggcatcgaca aggccgacat acgcttcgtt
     3961 atccactatc acctgcccgg ctcaatcgaa gcctactatc aggagatcgg gcgcgccggg
     4021 cgcgacggcc agcccgccac ctgcgtgctc ctcttcaact acgccgacaa acgcacgcag
     4081 gactacttca tcgaaggctc ttacccgccg cccgagatca tcgccaaagt gtacgaggct
     4141 ctcgtcgcca cgcggctgca acatatcgaa ctttcgaccc acgagatcgc cgtgcgcgcc
     4201 agcgtgcgca acgaaatggc ggtgcagtcc gcgctcatca ttctggagaa ggccgggcac
     4261 atcgagcgcg gcgcggcggg cgagaatcgc gccgcggtac gccttctgat gccgccgcat
     4321 ctcgcgcgtg aaaccgtcaa cgcgaaacgc agcgcacgcg acaagcaggt cttgttcaca
     4381 ctgctcggcg gctacgatct gaacgaacgc gccgactccg aaattgatgt caacgagttt
     4441 gccgaaactg ccggactcga tctcgcctcg gtgcgccgct cgctctcgtc gctcgccgct
     4501 gcgaaggtgc tctcgtacac gacggcgcga cgcacgcgcg ggctgcaaat gctcgacgag
     4561 cgacccgtca cacgtttgcg catccgcccg caagacctcg cccgccgcgc cgcgctcgaa
     4621 caacgcaagc tccgcgagat gatcagtttt tgctacgcgg atcgctgcta tcgcgccttc
     4681 atcctcgact acttcggcga ccgttcgcac aaaccgacct gcggcacctg cggcgtctgc
     4741 ctgagagaga agcctgcggc ggcaaacgac gcgcccgcgc tcgaagcccc ggacgattac
     4801 gtcgagagca gcttcgaaga tgaggtgtcg ccgttcagtt cgacgcgcga cgcttcggat
     4861 atacctccgg tgtctccccc gccctcttcg ccccgcacgc ggctcgacaa gttcgtcatc
     4921 gagaacacgc ctttcgcaag tgatctggag aacgaactgg atcggcagtc gcttgcggag
     4981 caccggcggc gcgaagccga aagcttcggc gcggagttaa acagcagcgt ccagattacg
     5041 ggcgcgcgcc cgctcggcca cgacgaaact ttgtgcgtgc gcaaaatcct cgcctgtgtg
     5101 gcgcgcatgg aagggcgttt cggcaagggc attctggcgg cctgcctgcg ggggtcgcgc
     5161 tcggcaaaga tctcacagtt cggcctcgaa cgcctctcga cctacggcat cctctccaac
     5221 atgacgcagg acgaaatact tctctacatc gatgcgctcg tcgccgcagg cgcgctgcgc
     5281 gtcgcaggcg gcaactaccc gaccgtcgcc atcacgagtt tcggaaacga cgtgatgcgc
     5341 gaacgcgcga ccatcgaact cgcgctcccg tcagtcgccc ccgtcgccgc gccgcctgcg
     5401 tccttcgcca tgcggggttc gcgcgacgcc gccgtcgcta acgtcaaagc ggttaatgcg
     5461 ccgcgcgtca gcacgattga cgagacttac gccttctaca aagcgggcat gaccatcgag
     5521 gagatcagcc gcgaacgctc catcgcggag atgaccgtgg aaaaacatct cgccgactgc
     5581 atcctcgaag gtcgtccctt cgacatctcc cgtcacgtca atgcggttga tcgcgcgctc
     5641 atcgaagagg ccgtccgccg tctcggcgat gaacggctca agcctctgcg cgatgcgctg
     5701 ccccgccaca tcagctatcg catgattcgc ttcgtcgtcg ccgatatgca acgcgccgcc
     5761 gccatcgctt caaacggcga cgcggaacgc gtgcggactt cggaagcgac aggcgtataa
     5821 actcccctcc gccgcctcgc gcgcttgacg tgccgtgcgc ccgccttcat catccataca
     5881 ttaaattcaa ccgacaggaa gaacagatga gccgcaaccc gaacaccgat ccgccgcaac
     5941 cggagcaaca acccgaactg cccacgccgc aaccgcacga tccgctgcga cccgacccct
     6001 cgcagcccga gcctctcccc ctgccgcccg gcggggagcc gaagtcgccc atcggtgagc
     6061 cggacgcgac gccgccgccc gcaggcgacc ctcccgtctc cgagccgacg cgtctggtct
     6121 agccctagct accgtataat aaatggagac tgtgcaagag gaagccgcgc acactaacgg
     6181 cacggggagc gtttcgggta cagcagcgca cgacgacgca cgcctcgaag agatcgcgtc
     6241 tgcgctgtcg gcaaaatctt tcacgccgca ccctctgttt cgcagcggcc acgcgcagac
     6301 gctcgtagcc tatgcatggc cgcgtcgcgg tcgtttgcgc gcgttgcaga cggatgaaga
     6361 gcgtctgttt gaagtcgagc cgggcgtgcg cgtgctcgtg cgttgtcgct ggcacgcaga
     6421 gcggcgcgcg catccgacgc tcctgctcgt gcacggactc gaaggctcaa gcgaatccat
     6481 ctacatgttg agcaccgcgc tcaaggcgca tcgcgcgggc ttcaacgtcc tccgcctgaa
     6541 catacgcacc tgcggcaaca cgctccatct cacgcccacg ctctacaaca gcggcatgtc
     6601 gccagacctg cgcgcgctca tctccgaact cgtcgggcga gacaagttga aggacatttt
     6661 cctcggcggc ttttcgctcg gcggcaacat gagtctcaaa ctggcgggcg aatacggcgc
     6721 agactttccg cgcgaattaa agggcgtcgc agttgtctcg ccttcggtcg agttggctgc
     6781 gtcggccacc tgcatcgagc agcgcgccaa ctggatttat caacgcaaat tcatccgcag
     6841 cctcaagcga cgcatgcgcg ccgccgcgcg cctctatccc gaacgctacg acgcgagcgg
     6901 acttcgacgc gtccgcacgc tgcgcgaatt cgacgaacgc tacaccgcgc cgcacggcgg
     6961 ctttcgcgac gccagcgatt attacgcgcg ctcaagttcg ctcccgctca tctcgcgcat
     7021 acgcgtgccg acgctcatca tccatgctca agacgatccg tttatcccct tcgcgtcgct
     7081 gcgccatcca tccgtccgcg caaactcctc gatcatcctg ctcgcgcccg cacacggcgg
     7141 acacgtcggc ttcgtcggcg acggacaaac ggcgggcgaa gatcgcttct gggcggaaaa
     7201 ccggctcgtc gaattttgcc gccgcatcaa cgaagggatg agggatgagg gatgaatcag
     7261 acaaagcaag ccggtcagct tgagtgcttt tctgattcat ccctcatccc tcatccctca
     7321 tccttttctg ttgacgtgtt gccgtgcagc gcaataatct tggagcaggt tttttaaggt
     7381 tgcagatgaa gatggagaac tgagagtcgg gtcggtcgct tccgcagcga agataaatga
     7441 ttactgcact caaattgatc gccaccgtgt tgatcgttgc catcaatgga ttcttcgtcg
     7501 cggcggaatt cgcactcgtc ggcgtgcgcc gttcgcgcat cgagacgctg gccgaagacg
     7561 gcgaccgccg tgcgaaacgc ctgctcggca tcctcgacaa tctcaacgct tatctctccg
     7621 cttcgcaact cggcatcacg ctcgcctcgc tcgcgctcgc catcctcggc gaaccgacca
     7681 tcgcgcacct gctcgaagcg cccttaagca gacttaacat cggcgacacg tggcgacacg
     7741 ccatcgcctt cgtcatcgct tacgcgatcc tgacctccct gcacatcgtc ctcggcgagc
     7801 aagcgccgaa actcgtcggc ctcgaacgcg cggagaaagt ggcgatgtgg tcggccttgc
     7861 cgatggagtt gttctacaag tttttcagct ggccgatccg cgctctcgat tgggcgagcg
     7921 cggcggttgt gcgtcccttc ggcatgaacg cgacgagcgc gcacgcttcg atctacacgg
     7981 aagaggagat ccgccaactt atcaacgctt cgcacaaagg cgggcatctc gaaatcgaag
     8041 agcagcaatt gatcaatcgc gtcttcgact tctccgaggc ggaagtgcgc gaagcgatga
     8101 tcccgcgcac cgccgtcgcc gccatccccg ttacggcgac actcgccgaa gtcgaagaat
     8161 ccttttgcga gacgggctac tcgcgcctgc ccgtctatcg cgaccaactc gacaacatca
     8221 tcggcgtcct cttcatgaag gacatcatgc cctgcctgaa aacttccggg cgcacggagt
     8281 tcgacatgga gaagatggtg cacccgccga tgttcgttcc cgccaccgcc cggctcggca
     8341 gcgtgctcgc gcagatgcag gccgcgcgca cgcatctcgc cttcgccata gacgagcacg
     8401 gcggcatcga aggcattcta acgctcgaag atttactgga agagatcgtc ggcgagatta
     8461 acgacgaata tgacgaggaa gtacgcgcac agatcatcga agagaacggc gcgtatgtgc
     8521 tcgacgggat gcttgccgtg cgcgacgcga atcgtaagtt caagttgaaa ctaccggagg
     8581 acgggggcta caccacgctc gccggattcc tgctcgcacg cgccggtcgc atcctgaaac
     8641 agggcgagac ggtcgagcac gacggggcga gtttcatggt cgagcgcgt
//
LOCUS       DASWNS010000555         5001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_72934, whole genome shotgun sequence.
ACCESSION   DASWNS010000555 DASWNS010000000
VERSION     DASWNS010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72934"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VGB76_19175"
     CDS             <1..198
                     /locus_tag="VGB76_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101055.1"
                     /translation="WTEQVALLYVLATLSVTALLLIVAFADLGGARQTLTSHAPNDDA
                     AAAGDGLGASQNASPRAATRR"
     gene            complement(345..1040)
                     /locus_tag="VGB76_19180"
     CDS             complement(345..1040)
                     /locus_tag="VGB76_19180"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY0101056.1"
                     /translation="MSTETVSQNEIIRKNISAISEMQRRDVAARKPQERISDSITRFS
                     GSTIFVFLHVIWFGLWILLNIGFIHLPYISGFDPFPFGLLTMIVSLEAIFLSTFVLIS
                     QNRMSALSEKRAELDLQVNMLAEQKAAKTLQLLEHIAQQLDTVYERFNYKTDPEIAAL
                     KVSPEPHEVLQVMEEAVEEQLKEVTQEIKKSVKDITGEVEAVQEEVQDVDKEVKRVAS
                     DVRDVQEEMKMRA"
     gene            1502..3598
                     /locus_tag="VGB76_19185"
     CDS             1502..3598
                     /locus_tag="VGB76_19185"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEY0101057.1"
                     /translation="MPRIITSLFLSALLLCAAVPPAGSYTYQYSSSSVQVRWMTTQIN
                     VALSSSLNSPPANIKPGSDVVGAARRALRRWSDASGIRLVETSTSVESVNPPNTSGDG
                     INLITVSASAINDTFTNTGGRHLGRTRVIFDPATGRIQEADLAINPRVTFSTDGTSGT
                     YDLESTFVHELGHLLGLDHSGVVGASMQPRQGQNFIDPAFTVRTLSDDDLAGIRSIYG
                     QPVAFPVGAISGHVNYGAGAHVWAENLYSGRVVGSSITKSDGSYRIDQLPPGDYRVVV
                     EYLNEPVVASEISLSPPYSGIGSQPPFRATEAQTSVAAGTTSILNLSVTLTAPSINPR
                     ILGINGALQGAPVQLAAGRTFRFTVGGNGLGNVPLTTTGFSVTSPFVVIDPASFVRED
                     PALYFPNPEPGYGIVSFTFTLVDTAKWGDYTLRLRSDAGEVAFLSGALAVDPYTDLVE
                     ANPLQNNGFFVRQQYLDFLFREPEPSGFNAWVGVLNRCDIAFSPDCDRVTVSAAFFRS
                     QEFQLKGYFVYRFYKLLLGRLPAYGEIIPDMISVTGATAGEVYQKRAAYANNWVQRPA
                     FTHLYGALSNADFVNTLMARYSLQTINTPDPNNPDTGGKVVFSRADLIGRLNAGTLTR
                     AQVVRAIVESDELFNAEFNPAFVAMQYFGYLKRDPDAGGYNAWLNYLNTNPTDFRTMV
                     NGFVNSNEYRSRFGQP"
     gene            complement(3724..>5001)
                     /locus_tag="VGB76_19190"
     CDS             complement(3724..>5001)
                     /locus_tag="VGB76_19190"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0101058.1"
                     /translation="TELANALCMAKNDGEREELALEHDASVVMLRPTNATPALLYIEP
                     RSAATLSGNLPIDSLLRVVELGMNVCALRARVSSGRGVQEQSTYTGTGLMPGFIHSSP
                     AMTQLVEEVHKIRSSDVTVLVTGESGTGKELVARAIHALSSRKAKVFVPFNCTAVPKE
                     LSEAYLFGYRRGAFTGAVSDSPGVIRSAAGGTLFLDEIGDLPLDVQPKLLRFLQEGEI
                     QPLGEQRPAKVDVRIIAATNSDLEKMVAEGRFREDLYYRLNVIRLRVPPLCERRSEIP
                     TIVNYYLNHYSAKFNRRDIQITPQTIDLLMVCDWPGNVRQLCNEIQRIVARAEDGTLI
                     TPDHLSPELKRISAPISLPSGVTPFGAGNAITLSGQHSTLADAMAELERRMIADALRR
                     HKGNISRAARELGLTRRGLYLKLERYSLSAASA"
BASE COUNT          957 a         1518 c         1471 g         1055 t
ORIGIN      
        1 tggacggagc aggtcgcgct gctctacgtt ctggcgacgc tcagcgtgac cgctctgctg
       61 ctcatcgtcg cgttcgcaga cctcggcggc gcgcgtcaga cgctcacttc gcacgcgccc
      121 aacgacgatg cggctgccgc cggagacggg cttggcgcat cgcagaacgc ttcgccgcgc
      181 gccgcgacac gtcgttaagt ctatttttcg gaagcacacg cccgaaccgg atgcgtgcta
      241 attaagagag cgggaggttc aatcaaagtt gaacctcccg ctcttttaat ttcccttgcg
      301 gagtcgcttt cgcacgcgcc cttcagcgaa ggttaaattc agtctcaggc gcgcatcttc
      361 atctcttcct gcacgtcgcg cacgtcgctc gccacgcgct tcacctcttt gtccacatcc
      421 tgcacctctt cctgcacggc ctcgacctcg cccgtaatat ccttgaccga ctttttgatc
      481 tcttgcgtaa cttctttcaa ctgttcttca accgcctctt ccatcacttg cagcacttcg
      541 tgcggttcgg gtgaaacctt gagcgcggcg atctccgggt cggttttgta gttgaagcgt
      601 tcatagacgg tatcgagttg ttgcgcgatg tgttccagca gttggagcgt cttggccgcc
      661 ttctgttcgg ccagcatgtt gacttgcagg tcgagttcgg cgcgcttctc gctcaaggcc
      721 gacatgcggt tttgcgagat gagcacgaag gtggagagaa agatcgcttc cagcgagacg
      781 atcatcgtga gcagcccgaa ggggaagggg tcgaatccgc tgatgtaagg caggtgtatg
      841 aatccgatgt tgagcagaat ccagaggccg aaccagatga cgtgaaggaa gacaaagatc
      901 gtcgagccgg agaagcgcgt gatcgaatcg gagatgcgct cctgcggttt gcgtgcggcc
      961 acgtctctgc gctgcatctc ggagatggct gaaatgtttt tgcgtatgat ctcgttttga
     1021 gaaactgtct cggtgctcat cgagtctttt acggcctcca tgaaatggaa tcggggagaa
     1081 aaatccgggt aaaaataagc ggcgaggctt tcacgtccga aagaagttcc tcattcctct
     1141 caaacaaact tcgtgccaac agcaccgcgc cgaaaagcta aagaaaagag cgcaagttga
     1201 atggtgtggc gcaaactttg tacccgtttg atgcgggcgg gcgtgcctgc gacccgaatt
     1261 tattaaccgc tgcctgacgc aactctcaaa cgcgcgccgc agatgcgact tgaaatagcg
     1321 gcattttgaa gcgtcgagag aagatgcttg tctccgtcgc atcagaactg ttataaattt
     1381 aagtgccctg ccggagcaac tccggttgag gaaattgccc gccaacaaac gcaggcaatt
     1441 tcgcatccaa aattttcccc cccacatatt ttttgatcgc ctcaagccaa agggagtttt
     1501 catgcctcga attatcacct ctttattcct gtccgcactg ctgctatgtg ctgccgtgcc
     1561 gcctgcgggc agctacactt atcaatacag cagttcatcc gtgcaggtca ggtggatgac
     1621 cacgcagatc aacgtcgctc tttcgagttc gctcaattcg ccgcccgcca atatcaaacc
     1681 gggcagcgac gtggttggcg cggcgcgcag ggctttgcgt cgctggtctg acgcttcggg
     1741 gattcgtctg gtcgaaacct ccacgagcgt cgagtccgtg aaccctccca acacgagcgg
     1801 cgacggcatc aacttgatca cggtttcggc ttcagcgatc aacgacacct tcaccaacac
     1861 gggcggcagg catctggggc gcacgcgcgt catcttcgat ccggccacgg gacgcataca
     1921 ggaagcagac ctcgccatca acccgcgcgt caccttttcg accgacggca catccggcac
     1981 ttacgatctt gagtccacgt tcgtgcacga actcggccac ctcctcggac tcgaccactc
     2041 cggcgtcgtc ggcgcgtcga tgcagccgcg tcaggggcaa aactttatcg atccggcctt
     2101 caccgtgcgc acgctttcgg acgacgatct ggcgggcatc cgctcgatct acgggcagcc
     2161 tgtcgcattt cccgtcggcg cgatctcggg tcacgtcaat tacggtgcgg gggcgcacgt
     2221 ctgggcggag aatctttata gcggcagagt cgtgggcagc agcatcacga agtcagacgg
     2281 atcgtatcgc atagaccaac tgccgccggg cgactatcgc gtcgtcgtcg agtatctgaa
     2341 cgaaccggtc gtcgcaagcg aaatctccct ctcgccgccc tacagcggca tcggcagcca
     2401 gccgcccttt cgcgcgacgg aggcgcaaac gagcgtcgcc gccgggacga cttcgatcct
     2461 gaatttgagc gtcacgctca ccgcgccttc catcaacccg cgcattctcg gcatcaacgg
     2521 ggcattgcag ggcgcgcccg tgcaactcgc cgccgggcgc acgttccgtt tcaccgtcgg
     2581 gggcaacggc ctcggcaatg ttccgctcac gacgacgggc tttagcgtca cctcaccttt
     2641 cgtggtcatc gaccctgcaa gtttcgtgcg cgaagacccc gccctctatt tccctaatcc
     2701 cgagccgggt tacggcatcg tgagcttcac ttttacgctc gtggatacgg cgaagtgggg
     2761 cgactacacc ctgcgcctgc gctccgatgc gggcgaggtc gcctttctgt ccggtgcgct
     2821 cgccgtcgat ccttacaccg atctggtcga agcgaacccg ctgcaaaaca acggcttctt
     2881 cgtgcggcaa cagtatctcg attttctctt ccgcgagccg gaaccgagcg ggttcaacgc
     2941 gtgggtcggc gtcctgaatc gctgcgacat cgccttctcg cccgactgtg accgcgtgac
     3001 ggtttctgcg gccttcttcc gttcgcagga gtttcaactc aagggctact tcgtctatcg
     3061 cttctacaaa ctcctgctcg gcaggcttcc cgcgtacggc gagatcattc ctgacatgat
     3121 cagcgtgaca ggagcgacgg cgggcgaggt ctatcagaag cgcgccgcct acgccaacaa
     3181 ctgggtgcag cggcctgcct tcacacatct ctacggcgca ctaagtaacg ctgacttcgt
     3241 gaacacgttg atggctcgct acagtctcca gaccatcaac acgcccgatc cgaacaaccc
     3301 ggacacgggc gggaaagtag tcttttcacg cgccgacctc atcggcaggc tcaacgcggg
     3361 aacgctcacg cgggcacagg tcgtgcgcgc catcgtcgag tcggatgaac tgttcaacgc
     3421 cgaattcaac ccggctttcg tcgccatgca atacttcggc tatctcaagc gcgaccccga
     3481 tgcaggtggc tacaacgcct ggttgaatta tctcaacacg aatcccacgg acttccgcac
     3541 gatggtcaac ggcttcgtca actcgaacga gtatcgcagc cgcttcggcc agccgtaaga
     3601 ttcaggagcg gcgcagaagt cgaattaagc gagagcgcac aaatcaaaat ggagaaggga
     3661 agcatccgcg ctttccttct ccattttgat ttgtgccttc gctgtcttgc gctgtcttgg
     3721 cgtctatgcg ctggcggcgc tgagcgagta gcgttccagt ttgaggtaga ggccgcgccg
     3781 ggtgagtccg agttcgcgtg cggcgcgcga gatgttgcct ttgtggcggc gcagtgcgtc
     3841 tgcgatcatg cgtcgttcga gttcggccat cgcgtcggcg agcgtggagt gctggccgga
     3901 gagcgtgatg gcgtttccgg ctccgaaggg cgtgacgccg gacggaagcg agatgggcgc
     3961 ggagatgcgt ttgagttcgg gcgagagatg gtcgggcgtg atcaaagtgc cgtcttcggc
     4021 gcgcgcgacg atgcgctgaa tctcgttgca caactggcgc acgttgcccg gccaatcaca
     4081 gaccatcagc agatcaatcg tctgcggcgt gatctgtatg tcgcggcgat tgaacttcgc
     4141 ggagtagtga ttgagatagt agttaacgat ggtcggaatc tctgaacggc gttcgcaaag
     4201 cggcggcacg cgcaggcgta tgacgttcag acgataatag aggtcttcgc ggaagcgtcc
     4261 ttcggccacc atcttttcga ggtcgctgtt ggtggcggcg atgatgcgca cgtccacctt
     4321 cgccggtcgc tgctcgccga gcggttgaat ctcgccctct tgcaggaagc gcaggagttt
     4381 cggttgcacg tcgagcggga ggtcgccgat ctcgtcgagg aagagcgtgc cgccagccgc
     4441 cgaacgaatc acgcccggag aatccgacac cgcgcccgtg aacgcgcccc tgcgatagcc
     4501 gaacaggtaa gcctcgctca actccttcgg aactgccgtg cagttgaagg gcacgaagac
     4561 cttcgctttg cgcgaagaga gcgcgtggat ggcgcgtgcg accagttctt tgcccgtgcc
     4621 ggattcgcct gtgaccaaca cggtcacgtc ggaagagcga atcttgtgca cctcttcgac
     4681 gagttgcgtc atcgcgggcg acgaatggat gaagccgggc atgagtccgg tgccggtgta
     4741 ggtgctctgt tcctgcaccc cgcgaccgga actgacgcgc gcccgcaacg cgcagacgtt
     4801 catgccgagt tcgacgacgc gcagcagtga gtcaatcggc aggtttccgg agagcgtggc
     4861 cgccgaacgc ggttcgatgt agagcagggc tggcgtggcg ttggtcgggc ggagcatcac
     4921 gacgctggca tcgtgttcga gagccaactc ctcgcgttcg ccgtcgttct tcgccatgca
     4981 cagagcgttc gccaactctg t
//
LOCUS       DASWNS010000556         8871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_203386, whole genome shotgun sequence.
ACCESSION   DASWNS010000556 DASWNS010000000
VERSION     DASWNS010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203386"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGB76_19195"
     CDS             complement(<1..414)
                     /locus_tag="VGB76_19195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0101059.1"
                     /translation="MAKKGSVLVVDDEEIMRDVLETLLSAEGYRVDLAKTGEEGLEAY
                     GRRAYDVVLLDVSMPGMGGLRALEEIQKVDAEAVVVMITAYATFDTAVAAWERGAFGC
                     IRKPFKNEQITSTIAAGIKRRRKEEERQTLRRAMGR"
     gene            complement(546..1721)
                     /locus_tag="VGB76_19200"
     CDS             complement(546..1721)
                     /locus_tag="VGB76_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101060.1"
                     /translation="MAHQRSLLRRAFSLAGARSPASASCAASLLLIIIASNFATVAAR
                     TEGGEAGVRVFEMSADGRTIIVRLRSFAPRASGRLEVEPTAGGGRVRLTAMNLPLPRT
                     LAPNARTFVAWASGGRILRLGELRRGSRGTGSLVFPHPQEFARYSLIVTAESDARAER
                     PTGAPVFSTRANEVAALFPLKDDAREAARRERRATTVRSNAPSAAAAAAPRTGAVRNT
                     TDAPKATTRAAAAAVNAAPPPAALRTRGRVRAGDSGDEFFTEIDEALRRDTGARTLTL
                     VGDSGARRARGTARVALQDGTAYVRVRFRGVPHPARFGRGRRYVMWALIPEDMATVYL
                     GSLPTRNLNRTQTYARTAGVNSDKFRLLVTAERRAAPAPRPRGRRVLMTFKGRRRKK"
     gene            complement(1911..4892)
                     /locus_tag="VGB76_19205"
     CDS             complement(1911..4892)
                     /locus_tag="VGB76_19205"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0101061.1"
                     /translation="MKSVLTTRAWVLLIAASLLVAAGALNLAQRLTRTPPPTDGVAWE
                     QTDKGIIALAVAPNSAASRAGILGILPGDRLISISLDEVHNDPVVAASEIQMYLEEAA
                     ETGHRLRYGIERPISDGQTRDYYVYLDNLQPIPNRTTRDYYINFIGVVYLLVGLFVLF
                     KQGGRAPFVIHFTTLCLVAFVLHFYRPIGFYEDLDKTIAFLDSAAYVLFAPLFLHFCA
                     IYPVRRQLLERSHKISVLLYAPAIILVLVNALVYYPVELEFLFRKFHLNLHAPTFPEG
                     LLATLYKVEFAHFWGALVVGASVLVRRFWKSKVAVVRQQLKWVVWGSVLAVTPFSLLY
                     AVGYLFGDAAATTDVGAERWLIDAAVLPLILIPLTFGNSVVRYRLMDVDVVVRRAAVY
                     ALTTLAIALMIGVVVYVAGLYAFGGEVVTPGVITLRVIIAVLAMTVIVMMAAPIKNFL
                     QEQTDRLFYGERYDLRHGLLDFGRTLSATTTLEPLLDALISRLQQVLGVARVAIFIED
                     ARAASGYRVARSVGLSSEVIVPQDFREMIRVRSADAGIVRADDIDMQPETSGFVRRAL
                     HYYVPCVVRGRMIAVIGLGRSTGGALLSSEDLEILRTISGYVAVAIENSLLYQEQTER
                     AAELELLKEFNESIVESINVGLLAVDSAGQITGCNTALEELLGVRRDEAIGAHVEDLF
                     ADDFAETLEQALGENGWELTSLRHIYKLHTATREGASLILNIAFAPLRRTEIAAHTGA
                     LVVLEDVTARVRLEEQLQQREKLSSIGLLAAGVAHEINTPLTGVSSYTQMLLGMLATS
                     DPKHKLLEKIHRQTERATGIVNNLLNFSRTGLVTEFDEVDVNRVLDDTLQLLEPQLRQ
                     SRIEIVRDYDARLPAAYGNGGKLQQVFTNLILNARDAIPDGGRITLSTATEDGESISV
                     LVADTGIGIAPENVARIYDPFFTTKGIGRGTGLGLAVSYGIVQEHSGHINVQSAPGRG
                     TTFRITLPTSQGRARLQAASD"
     gene            complement(5265..7076)
                     /locus_tag="VGB76_19210"
     CDS             complement(5265..7076)
                     /locus_tag="VGB76_19210"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0101062.1"
                     /translation="MRKTYWRVSLTLLFALFLFSEAGAPAATYAASAVPSGGKLGVIS
                     GSVRDHKGNPLAGAIVQLLRDGADHISKQTRTGSDGTFSARIVPGRYLLTALAEGFNA
                     TTFNAVQVNPSDELVYRFNLEPLGQGRTTPERRNDRNDPKWRLRSSQNRRSVFQHEGA
                     QDATIAAVEEALEAKENAEEAAQGSGAESADDWAQNGQRVQGVIETFTATSANPNAPV
                     YAGTNFAVSVPANEGLNFIFAGQLGTNQLARLETTARFRVGANHRFSTTIGGANLPLA
                     FNSAGTTTGATPPLRALGQVSVRAVDEWIVRDGVVVVLGLDYARFTNADGEGMFSPRF
                     GLQLETDARTRLRFGYTPGGVNSQPQSAAQFEDGAIVFPEMDEQSIAFVDGRPLMERS
                     RRLEFGVERVLDNNSSIEATAFFDTTDGRGVGLMNMPLGAFAGERGEQLLSVANQQGA
                     ARGLRVVYARRLGNFLKTSAGYSFGRGQQLAAGFSANQSSPGDLFDNGFFQVLAAQVE
                     SSFDTGTDVRTVLRFSPRAAVFAIDPFAGRVAVFDPSLSILVTQDLPTFGLPVRAEAV
                     IDARNLLDAQTSVDDGETLTSLSTNRRSVRGGISVRF"
     gene            7442..8731
                     /locus_tag="VGB76_19215"
     CDS             7442..8731
                     /locus_tag="VGB76_19215"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0101063.1"
                     /translation="MTEAILSTKLSHGATVLTEQMPGLRSATIGIWVKRGSRHETPDF
                     NGICHFIEHAVFKGTERRSVLDIAIESDRLGGNFDAYTSHEMTGFALKVVDRALPQAF
                     DLLSDMLLRPRFDEVELEREQKVIIEEMKMVEDSPEEFLGELFHAAYYPDHALGRPIE
                     GTPETVSSFNHARTTEFHRTAYHPRNFVIAAAGNVSHAELVELAERAFAGREPTDAQH
                     LSQTEENAATPPRPAAPILIQRKRELEQAHLILATPWPSARDEDRYAASLLGSVIGGG
                     TSSRLWQSVREERGLAYSVGAAAQGFTDTGLFQIYAGTSPAQLDEVLSLALMELRRVV
                     REPVSHEELQLVKDQAIASILLGLESTSARAGSLARQEITHGRRISPDEIIARLEAVT
                     PEDMQRVARARFTSDTLALGALGNLNGFKVSRSRLEI"
BASE COUNT         1720 a         2746 c         2818 g         1587 t
ORIGIN      
        1 gcgtcccatc gcgcgccgca aggtctgccg ctcctcttcc ttgcgccgac gcttgattcc
       61 ggcggcgatg gtcgaggtga tctgttcgtt cttgaacggc ttacggatgc agccgaaagc
      121 gcctcgctcc cacgcggcaa ccgccgtgtc gaacgtcgca taagccgtga tcatcacgac
      181 gacggcttcg gcgtcaacct tctgaatctc ttcgagcgcg cgcaagcccc ccatgccggg
      241 catggacacg tccaagagga cgacatcgta agcgcgccgc ccgtaggctt ccaagccctc
      301 ttcgcccgtc ttcgccagat caacgcgata gccctccgcc gagagcaacg tctccaacac
      361 atcacgcatg atctcctcgt cgtcaacgac gagcactgaa cccttcttag ccataaagca
      421 attaaaacag cgacgggtga caagtgacaa gtgacgagag aagagaagtg atgagtaatg
      481 ggtaatgagt gatgagttaa gacaaaagcc ttgtctttga ctcatcactc attactcatc
      541 actcatcact tctttcttct ccggcctttg aaggtcatca gcacgcggcg gcctctgggg
      601 cgaggggcgg gggcggcgcg tcgttcggcg gtgacgagga gacgaaactt atcggagttg
      661 acgcctgcgg tgcgcgcgta ggtctgcgtg cggttcaagt tgcgcgtggg caggctgccc
      721 aggtagaccg tagccatgtc ttcgggaatc aaagcccaca tcacgtagcg gcggccacgg
      781 ccaaagcgcg ccggatgcgg gacgccacgg aagcgcacgc gcacgtaagc cgtgccgtct
      841 tgcagtgcga cacgcgccgt cccacgcgca cgacgcgcgc cgctatcgcc gacgagcgtg
      901 agcgtgcgtg cgcccgtgtc gcgcctgagc gcttcgtcaa tctccgtgaa aaactcgtcg
      961 ccggagtcgc ccgcgcgcac gcgtcctctc gttctcaagg cagcgggcgg cggggcggcg
     1021 tttacggcag cggcggcggc gcgcgtggtc gccttcggcg cgtccgtcgt gtttcgcact
     1081 gcgcccgttc gtggagcggc ggcggcggcg gcggaaggcg cattgcttcg cacggttgtg
     1141 gcgcgacgtt cgcggcgtgc ggcttcgcgg gcatcatctt tgaggggaaa gagcgcggcg
     1201 acttcgttgg cgcgcgtgga gaagacgggc gcgcccgtcg ggcgttcggc acgcgcgtcc
     1261 gactcagcgg tgacgatcag gctgtagcgc gcaaactcct gcgggtgcgg gaagacgagc
     1321 gagcctgtgc cgcgcgagcc gcgccgcaac tcgccgagac gcagtatgcg accgccgctc
     1381 gcccacgcga caaacgtgcg tgcgttcggc gcgagcgttc gcggcagagg cagattcatc
     1441 gccgtcaggc gcacgcgccc gccccccgcc gtcggctcaa cctccagacg cccgctcgca
     1501 cgcggcgcaa acgaacgcag acgcacgatg atggtgcgcc cgtcagccga catctcgaac
     1561 acacgaacgc ccgcctcgcc gccttcagtg cgcgcggcga cagtcgcaaa gtttgacgcg
     1621 atgatgatga gtaacaaaga cgcggcgcag gaggccgacg ccggagaacg cgcgcccgcc
     1681 agactgaacg cccgcctcag gagacttctc tggtgtgcca tgaagcaaaa gaccttacgg
     1741 ctcaagttgt gggatcgaac ggctgcgaga gtacaccaat aaaagtaaat cgtgaacgta
     1801 aatcgtaaac agattcaaaa gccgtgaatc gttgatcgtg aatagatgaa aacctgtctt
     1861 tgtctattga cgattgacga ttcacggctt ttaacgattg acgccatttt ttaatcgctc
     1921 gcggcttgca ggcgtgcgcg cccctgcgaa gtggggaggg tgatgcggaa ggtcgtgccg
     1981 cgaccgggcg cgctctgcac gttgatgtgg ccggagtgct cctgcacgat gccgtaggag
     2041 acggcgaggc cgagtcccgt gccgcgcccg atgcctttcg tcgtgaagaa aggatcgtag
     2101 atgcgcgcga cgttttccgg cgcgatgccg atgcctgtat cggcgacgag gacggagatc
     2161 gattcgccgt cttccgtcgc ggtcgagagc gtgatgcgac cgccatcggg aatggcatcg
     2221 cgcgcgttca ggatcaggtt ggtgaagacc tgctgtagtt tgccgccgtt gccgtaagcg
     2281 gcggggagcc gggcgtcgta gtcgcgcacg atctcgatgc ggctctggcg cagttgcggc
     2341 tccagaagtt gcagcgtgtc gtcgagcacg cggttgacat caacttcgtc aaactccgtg
     2401 acgagtcctg tgcgcgagaa gttgagcagg ttgttgacga tgcctgtggc gcgttcggtc
     2461 tggcgatgaa ttttttcgag cagcttgtgt ttggggtcgc tcgtggcgag catgccgagg
     2521 agcatctgcg tgtaactgga gacgcccgtg agcggcgtgt tgatctcgtg cgcgacgccc
     2581 gccgcgagga ggccgatgct cgatagcttc tcgcgctgct gcaactgctc ttcaagacgc
     2641 acgcgcgccg ttacgtcttc gagcacgacg agcgcgcccg tgtgcgcggc gatctcggtg
     2701 cggcggagcg gggcaaaggc gatgttgaga atgagcgatg cgccctcgcg cgtggccgtg
     2761 tggagtttgt agatgtggcg cagggaggtc agttcccaac cgttttcgcc gagcgcctgt
     2821 tcgagcgtct ccgcgaaatc gtccgcgaag aggtcttcga cgtgtgcgcc gatggcctcg
     2881 tcgcgtcgca cgccgagcaa ctcttcgagc gcggtgttgc agcccgtaat ctgtccggcg
     2941 gaatccacgg ccagcaggcc gacgttgatg gattcgacga ttgactcgtt gaactctttg
     3001 agcagttcga gttcggcggc gcgttcggtc tgttcctggt agagcaggct gttctcgatg
     3061 gcgacggcga catagccgga gatggtgcgc aagatttcca aatcttccga agagagcagc
     3121 gcgccgcccg tcgaacgccc gaggccgatg acggcgatca tgcgcccgcg cacgacgcaa
     3181 ggcacatagt agtggagcgc gcggcgcacg aagccgctcg tctccggctg catatcgata
     3241 tcgtcggcgc gcacgatgcc cgcgtccgcc gaacgcacgc gaatcatctc gcggaaatcc
     3301 tgcggcacga tcacttccga agacagtccg accgaacgcg ccacgcgata gccggaggcc
     3361 gcccgcgcgt cttcgatgaa gatggcgacg cgcgccacgc ccaacacctg ttgcaggcga
     3421 ctgatgagcg catcgagcaa aggttcgagc gtggtcgtgg cggagagcgt gcgcccgaag
     3481 tcgagcaggc cgtggcgcag gtcgtagcgt tcgccgtaga agaggcggtc ggtctgttcc
     3541 tgcaagaagt ttttgatcgg cgcggccatc atcacgatca cggtcatggc gagcacggcg
     3601 atgatcacgc gcagggtgat gacgcccggc gttaccacct cgccgccgaa cgcgtaaagc
     3661 cccgcgacgt agacgacgac gccgatcatc agagcgatgg cgagggtggt gagcgcgtag
     3721 acggcggcgc ggcgcaccac cacgtcaacg tccatcaggc gatagcgcac gacggagttg
     3781 ccgaaggtga gcgggatcag gatgagcggc agcaccgccg cgtcaatcag ccaacgctcc
     3841 gcgcccacgt cggtcgtggc cgccgcgtcg ccgaagagat agccgacggc gtagagcagg
     3901 ctgaaaggcg tgaccgccag caccgatccc cacacgaccc acttcaactg ctggcgcacg
     3961 acggcgactt tgctcttcca gaaacggcgc acgaggacgc tcgcgcccac gacgagcgcg
     4021 ccccagaaat gcgcgaactc caccttgtag agcgtggcga gcagaccttc cgggaacgtc
     4081 ggcgcatgga ggttcaggtg aaacttgcgg aagaggaact caagttcaac cggatagtaa
     4141 acaagggcat tgacgaggac gagaataatg gccggggcat agagcagcac cgaaattttg
     4201 tggctgcgct ccagcagttg acggcgcacc ggatagatcg cgcagaagtg caggaagagc
     4261 ggcgcgaaga ggacgtaggc cgcgctgtcc agaaacgcga tggtcttgtc caggtcttcg
     4321 taaaagccga tggggcggta gaagtgcagg acgaaagcga cgaggcagag cgtcgtgaaa
     4381 tggatgacga agggcgcgcg cccgccctgt ttgaagagaa cgaagaggcc gacgagcagg
     4441 tagacgacgc cgatgaagtt gatgtagtaa tcgcgcgtcg tgcggttcgg gatgggttgg
     4501 aggttgtcta gatagacgta gtagtcgcgc gtctggccgt cggagatggg gcgttcgatg
     4561 ccgtagcgca ggcgatgccc cgtctcggca gcctcctcca gatacatctg aatctcggag
     4621 gccgcgacga ccggatcgtt gtgcacttcg tcgaggctga tggagatgag gcggtcgccg
     4681 gggaggatgc cgaggatgcc cgcgcggctg gccgcagagt tgggcgcaac ggcgagcgcg
     4741 atgattcctt tgtcggtctg ctcccaggcc acgccgtcgg tggggggcgg cgtgcgcgtc
     4801 aggcgttggg cgagattgag tgctccggca gcgacgagca gcgatgccgc gataagcagc
     4861 acccacgccc gcgttgtcag caccgatttc atcttccgcc ttcgctcctt tcaacaacca
     4921 aaacgacaaa acaaaggcaa ttgatgttcc agtcaggacg gagaaggata ccgctttcgt
     4981 gcccacgcac gagcaaggtg caacgcggcg catcgcctat gagtagggcg ttttacctca
     5041 ctcgccgtcc agcccggctc cacccctgct ataaaagccg aataaactta aatccaactt
     5101 tttgcattta tccgagattt cggaaaaata agtttttgct ggcttatagc gactgtccgg
     5161 cgaaagcatt accgcagaga aagaaaatga tgaacgcgga atgaaagcac taacgcgctt
     5221 gagctttcat tccgcgttca tcgttcctac ttcatcgttt ctctttagaa gcggactgag
     5281 atgccgccgc gcacggagcg acgattggtg cttagtgagg tgagggtttc gccgtcgtct
     5341 acgctggtct gcgcgtcgag caggttgcgc gcgtcgatca cggcctcggc gcgcacgggc
     5401 aggccgaagg tcggcaagtc ctgcgtgacg agaatactca aactgggatc gaagacggcg
     5461 acacgtccgg cgaagggatc gatggcgaag actgcggcgc gcggtgagaa gcgcagcacg
     5521 gtgcgcacgt cagtccccgt atcgaaactc gattcgactt gcgcggcgag gacttggaaa
     5581 aagccgttgt cgaagaggtc gccgggcgac gactgattgg cggagaagcc tgcggcgagt
     5641 tgttgcccgc gcccgaagga gtaaccggcg gaagttttca ggaagttgcc gaggcgtcgg
     5701 gcatagacga cgcgcaggcc gcgcgccgcg ccctgctggt tggcgacgct caagagttgc
     5761 tcgccgcgct caccggcgaa agcgcccagt ggcatgttca tcaagcccac gccgcgcccg
     5821 tccgtcgtgt cgaagaaggc cgtcgcttcg atgctcgaat tgttgtcgag gacgcgttcg
     5881 acgccgaact ccaagcggcg gctgcgctcc atcaagggtc gcccgtcaac gaacgcgatt
     5941 gactgttcgt ccatttcggg gaagacgatg gcgccgtctt caaactgcgc cgccgattgc
     6001 ggctgcgaat tgacgccgcc cggcgtgtag ccgaagcgca ggcgcgtgcg cgcgtccgtc
     6061 tccaactgta aaccgaagcg cgggctgaac attccctcgc cgtctgcgtt cgtgaagcgc
     6121 gcgtagtcca gaccgagcac gacgaccacg ccgtcgcgca cgatccactc gtcaacggcg
     6181 cgcaccgaaa cttgtccgag cgcacgaagc ggcggggtcg cgccggttgt cgttcccgct
     6241 gaattgaagg cgaggggcag atttgcgccg ccgatggttg tgctgaaccg gtggtttgcg
     6301 cccacgcgga agcgcgcagt ggtttccaga cgcgcgagtt gattcgtgcc gagttgaccg
     6361 gcgaagatga aattgagtcc ctcgttcgcg ggaacggaga cggcgaagtt ggtgcccgca
     6421 tagacgggcg cgttcggatt ggccgacgtg gcggtaaacg tctcgatgac gccctgcaca
     6481 cgctggccgt tctgcgccca atcgtccgcg ctctccgcgc cggagccttg cgctgcctct
     6541 tccgcgttct ccttcgcctc cagtgcttcc tcaaccgccg cgatggtcgc atcctgcgcg
     6601 ccttcgtgct ggaagacgga gcggcggttt tgcgacgagc gcagacgcca cttcggatcg
     6661 ttgcggtcgt tgcgacgctc aggcgtggtg cgcccctgtc ctaaaggttc gaggttgaag
     6721 cgatagacga gttcgtcgga tggattgacc tgaacggcgt tgaaggtggt ggcgttgaac
     6781 ccttcggcga gcgcggttaa gaggtagcga ccgggcacga tgcgcgccga gaaagtgccg
     6841 tccgagcccg tgcgtgtctg tttgctgatg tggtctgcgc cgtcgcgcag caattggacg
     6901 atagcgcccg cgagcggatt gcccttgtgg tcgcgcaccg aaccgctgat gactccgagt
     6961 ttgccgccgc tcggtactgc cgaggcggca taagttgcgg cgggcgcgcc cgcctcgcta
     7021 aacaagaaga gcgcaaacag cagcgtcaaa ctcacacgcc agtaagtttt tctcatcgcc
     7081 ccaaaacctc ccggcgcacc ttctcacgtc tccggaaaac ggtgcgccct caagcgacta
     7141 ttcacttgtc caaatggacg gcggcacgga agactcaacc tagctgccgg gtaaaatgct
     7201 ggccgcgcga aagccgcgcg aacccgtctg cctgcctgat atatgaccgg cctgaacccg
     7261 cttgagcgcg aaggtggcta ttttccgtca actgccaaac cctgtcaagc aggccgtttg
     7321 ccgctggcgg aggcgtctcc ggcaggtgtt tgccgcgaat ctgttcgcat gctacgatgc
     7381 tccttactgc aaaagtgatc accaacccgt caaaaatttt cgcatctaag ttcaacacgg
     7441 catgaccgaa gcaatactct caaccaaact ttctcacggc gcaaccgtcc taaccgagca
     7501 gatgcccggc cttcgctccg ccaccatcgg catctgggtc aagcgcggct cgcgccacga
     7561 gacgcccgac ttcaacggta tctgccattt catcgaacat gccgtcttca agggcacgga
     7621 gcggcgcagc gtcctcgaca tcgccatcga atccgaccgc ctcggcggca atttcgacgc
     7681 ctacacgtcg cacgagatga ccggctttgc cttgaaagta gtggatcgcg ccctcccgca
     7741 agccttcgac ctgctctccg acatgctcct cagaccgcgc ttcgacgaag ttgaactcga
     7801 acgcgagcaa aaagtgatca tcgaagagat gaagatggta gaagactccc ccgaagagtt
     7861 tttgggcgaa ctgttccacg ccgcctatta tcccgatcac gcgctcggtc gtcccatcga
     7921 gggcacgccc gaaaccgtct cctctttcaa ccacgcgcgc acgacggagt ttcaccgcac
     7981 ggcttaccac ccgcgaaact tcgtcatcgc cgccgccgga aacgtctccc acgccgaact
     8041 cgtcgaactg gccgaacgcg ccttcgccgg acgcgaaccg acggacgcac agcacttatc
     8101 tcaaacggaa gagaacgcgg ccacgcctcc gcgccccgcc gcgcccatcc tcatccagcg
     8161 caaacgcgaa ctcgaacagg cgcacctgat tctcgccacg ccctggcctt cagcgcgcga
     8221 cgaggatcgc tacgcggcga gcctgctcgg ctcggtcatc ggcggcggga catccagccg
     8281 tctctggcaa tcggtgcgcg aagaacgcgg cctcgcctac tcggtgggcg cggcggcgca
     8341 gggcttcacc gacaccggcc tcttccaaat ctacgccgga acgtcgcccg cgcaactcga
     8401 cgaagtgctc tcgctcgcgc tcatggaact gcgccgcgtc gtgcgcgaac ccgtcagcca
     8461 tgaagagttg caactcgtga aggatcaggc catcgcctcg atcctgctcg gactcgaatc
     8521 cacgagcgcg cgcgccggtt cgctcgcgcg tcaggagatc acgcacggac gccgcatctc
     8581 gcccgacgag atcatcgcgc gcctcgaagc cgtcacgccc gaagatatgc agcgcgtcgc
     8641 ccgcgcccgc ttcacttcgg acaccctagc cctcggcgcg ctcggcaacc tcaacggctt
     8701 caaagtgtct cgctcgcgtc tggaaattta gaaacagtgc caagtgtcga gtgacgggtg
     8761 acaagttaaa acgacaaaac ttgttcttaa aactcgttct tagacttctc ttcaactcgt
     8821 cacttgtcac tcgccactgc tttatgaaat ttaccgtcct cggaagcggc t
//
LOCUS       DASWNS010000557        16944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_21484, whole genome shotgun sequence.
ACCESSION   DASWNS010000557 DASWNS010000000
VERSION     DASWNS010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16944
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21484"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4045
                     /locus_tag="VGB76_19220"
     CDS             <1..4045
                     /locus_tag="VGB76_19220"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0101064.1"
                     /translation="KRLRVARVEWAKTSFDSWWSGASAGFKSDASTEGFDYQLAEINQ
                     LAADDTWTPTKALPEATGTAVWTGTEMIVWGGGKQNSGARYNPATDTWTPTPVTNAPS
                     HRSGHSAVWTGTEMIVWGGCERHFDFCGTNDGGRYNPVSDTWTRTSLANAPTPRSRHV
                     AIWTGSKMIIWAGLHHGYNNGGRPINSGGVYDPASDSWTTMNTTNAPENGGSGFKAVW
                     TGSEMIVWGGNTSVGGRYNPNTNSWTPTNPLNAPSQRADHTAIWTGTEMIVWGGTSGI
                     EPLNTGGRYNPANDSWTPTSTAGAPSPRYLHTAVWTGTEMIVWGGSAPLPVNTGGRYN
                     PSSDSWTPTNNANAPPPKSGHVAVWTGTEMIVWSVTENKTGGRYNPANDSWTPTDNND
                     SPPPVNFGVWSGAEMIIWSPTSACLSGCPAAGKRYDPATYTWRPMSNVGAPIPPPADR
                     PIVAVWTGTEMIVWGANDGQYTTPGEGGRYNPQTNSWQPVTTSGAPVNRAYFAGVWTG
                     TEMVVWGGQSNEGTMQNTGGRYNPSTNTWRPTSTAGAPEARYIHSAVWTGSEMIVWGG
                     SAGSLAHLNTGGRYNPANDTWTPTTTSGAPEARRFHTAVWTGAEMIIWGGRIGDYISS
                     DGLFNTGGRYNPSTDSWTPTSTTGAPAPRFKHTAVWTGELMIVWGGIATNELGYALST
                     NTGGRYQPGTDTWTPTSTLRAPGRRHDHVALWTGTGMLVWGGYAEDGSATHGGYYFAS
                     GEANGNTPPTVRITAPSDDATFEAGANVQITTETGDADGTVASVRFYANDTLLGSDST
                     APFDYSWSGVPAGSYTVKAVATDDSGGATTSAPVSFKAIVPAATANLSLGVVDSPDPI
                     TVGGNLTYALTVNNGSQAGASNVVVTATLPANAAFVSATNGCTRSGNTVACALGQLDQ
                     GANRALSIVVTPNVAGTLNFAASVASDTGDPDASNNAAQAATDVHLRGTDVKMGDVLI
                     SEFRAHGSAGVDAELDDFVELYNNTNVAITVGPVDASGGWALVAREANGLPVVRATVP
                     KGTVIPARGHYLVTAAAYSLGALAAGDLPVAGNILDTGGVALFRTADPAKFGAMEERL
                     DAAGFNSVSDQMYREGSGLTPFDAVQAEQSFVRKLNSVGQPQDTGDNAADFQLISKTP
                     GSFGAVPAILGTPGPENTGSPIQRNASVKPSLVDPGCAGFGAATSGCARVREGGTVAN
                     GAFGTLTIRRRFTNKTGAAINRLRFRITDVTTLGTPGTNALADLRAISSVDVEATVNN
                     NPVIIKGTLLESTHTLGGGVNTTLTIPLAGNSLAPNASIDVQFRLGVERNGAFRFFVN
                     VEALTTALLEEGAGAKSGSPTKQNPDK"
     gene            4193..5911
                     /locus_tag="VGB76_19225"
     CDS             4193..5911
                     /locus_tag="VGB76_19225"
                     /inference="COORDINATES: protein motif:HMM:NF015530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101065.1"
                     /translation="MRYRMMLTSSVLLSALCFNSLALPASAVARQKRRAPKAAAVSTA
                     RTSLDAKIRRFAPTTLTADTSRLSAGDRRALARIIEAAKLLDPLYLRQVWSGNPALKR
                     RLEADQSAEGRARLHYFNINYGPWSRLDDNEPFVEGVPHEKPKHANYYPDDMTKEEFN
                     AWVQTLKAEEKARATGFFHLIRRDANGKLKTVPYSVEYREFLEPAARLLRQAARETTN
                     ATLKAFLAKRADAFLSDNYYESDVAWMDLDAPLDVTIGPYETYEDELFSYKAAFESYV
                     TLRDEAESAKLAKFSGYLQELEDNLPMEARYRNPKLGAASPIRVVNELFSSGEGRRGV
                     QTAAFNLPNDERVVREKGSKRVMLKNVQEAKFNKTLLPISRVVLTPAGQRALSFESFF
                     THILAHELMHGLGPHNIKAGGGETTVRMQLKELYSAIEEAKADITGLWALQYLIDKGL
                     VEKRMERSLYTTFLASCFRSVRFGITEAHGKGIALQFNYLTDEGAIKFNERAGTFEID
                     ESRIKAAVAKLTREILTLQAEGSYERAKALLDKYGVIRPPMQRALARLGDVPVDIEPH
                     FPLAPG"
     gene            complement(5940..6941)
                     /locus_tag="VGB76_19230"
     CDS             complement(5940..6941)
                     /locus_tag="VGB76_19230"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HEY0101066.1"
                     /translation="MSLLPETMSRPFFYCSDLSREAAEGSFGTASIGDVWLLVEYREG
                     WGAHAFEESTLSAQVKRHLGNFCKLVPRSRLLFIKQERLRRDALSCFIVRCREGSPSI
                     VRFTFSSHEELLNLDLAAANAAAGDSLAGGVRTDEPLYLVCTHGKRDKCCAKFGYALY
                     KSLREGATAERERVWQSSHVGGDRFAANLICFPHGLFYAHVTEEAGRTILREYEHGRR
                     LVLAGYRGRTCYSYPVQAAEFFIRRETGINAIGELRYRRHERTGEKSWLVKFSEAGAG
                     KIHEAHVAARTSEFHNYITCNSTGEQRVVQYALESYRATDNDAPPSPPSQPQPAPVE"
     gene            complement(6960..8138)
                     /locus_tag="VGB76_19235"
     CDS             complement(6960..8138)
                     /locus_tag="VGB76_19235"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0101067.1"
                     /translation="MEPHTAEEFLASSWGQTYRHIRGRAGRFASLLPWERLNEILRQH
                     RLDFPRLRLMREGRALPVASYLRHTSGARSKQTIPRLQPVKFTAELRAGATLVLDAVE
                     ELHAPLEALAAGLELLFQERVQINSYAGWHTSRGFDLHWDDHDVFILQVTGRKRWSIY
                     GETRPHPLSGDADLPKPSGEPLWQATLEDGDLLYIPRGWWHVAEPLDEPTLHLTVGIH
                     KRTGIDLLAWLAGQLRASELFRQDLPRFATPAERAAHVERLGEELRAAWDAGLLERYF
                     KEQDAQAEPRAQVSLPWSALTQPVPADQDVRVRLVAPRPLDLQVVDGVVEFCCHKKRW
                     RFAAGALPVLRRLADARTAALSVSELCEAAGGRLDEQTVRAFLGELLRHGLVRTVAKD
                     "
     gene            complement(8193..8537)
                     /locus_tag="VGB76_19240"
     CDS             complement(8193..8537)
                     /locus_tag="VGB76_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101068.1"
                     /translation="MSEQINSGQVEQPETTDASGLLLSVEPLQASAHLSAGDDSSDTS
                     NDGGSDTGDDSSDTDSSDSTGSDSLDSTGTSTDTDLTDSLGADDGSDDSSDSDGTDDS
                     DASVITIDSKNA"
     gene            9068..9463
                     /locus_tag="VGB76_19245"
     CDS             9068..9463
                     /locus_tag="VGB76_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101069.1"
                     /translation="MLKKIRLGDDVQSYCGRCKEERMHQVVALHSDGRIERVTCNYCQ
                     STHRYRDPQATAAAAVKTRRTTGARASEARERAVSDAPARPYSPQESYAAGERITHPK
                     FGEGSIVEARQGKIDVRFGKELRTLLHAG"
     gene            complement(9545..11785)
                     /locus_tag="VGB76_19250"
     CDS             complement(9545..11785)
                     /locus_tag="VGB76_19250"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0101070.1"
                     /translation="MLAETQKINILMVDDSPTNLLALEAILQAPDRNLVRAASGDEAL
                     RYLLEGEVAVILLDVYMPGIDGLETAALIRGREKSRDIPIIFLTADSTGNRHIARGYS
                     LGAVDYILKPVDPDILRSKVAVFVELYKKTEEIKRQAALLHEKNIELENANLQRLGKL
                     IDLGQELAAERDPEQVLEKFCSAAREIVGAEHAGVGVLDEHGGSLRYYCHCDVCERGD
                     DNVNAPQVSESVLGQLIAEGRPLRLSEVAQTLEAEHLSSPHVSMQSFLGAPILSPARA
                     YGWLYLTNKLDAEEFSEADERLAVTLATQVSASYENARLYTEAQRHATELQQEVTERK
                     QAEEERARLLIREQAARAEAERANRTKDEFLATLSHELRTPLTAILGWTHLIRGGNLD
                     MSMMARALETIERNARSQSQLIDDLLDVSRIITGKLALDFHPVELSAIIEATIDTVRP
                     MAEAKSIHVKPQLKTSHCMVSGDPARLQQVVWNLFSNAVKFTPEGGHVEVGLKCDERH
                     VELEVRDTGQGIAPEFLPYIFDRFRQADGTTTRKHGGLGLGLAIVRHLVELHGGAIRA
                     VSDGDGQGATFTLSLPLASGAQASGNGAPAPDAATEVEAHGSLAKSAVLEGLRILVVD
                     DEADTRELVQEVLGRQGAEVQTSGSVAEALRTLAQWQPDLLVSDIGMPFEDGYELIRR
                     VRKLYGDGARGQIPAVALTAYAGVEDSKRALAAGFQMHLAKPVDPKALISTLARLAGR
                     ETPVDA"
     gene            complement(11779..12360)
                     /locus_tag="VGB76_19255"
     CDS             complement(11779..12360)
                     /locus_tag="VGB76_19255"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY0101071.1"
                     /translation="MTVELVVVGTSYGGLAALQILLPALARDFPLPIVVVQHRGKDSD
                     NLCEFLQKYCALPLSEPNDKEAIAPGRVYLAPRDYHLLIERDGFALSTEAPVGYARPS
                     VNVLFESAADIYQERTLGLILTGANTDGARGLAKIKSYGGLCLVEDPSSAESRGMPQA
                     AIAATMVDAILPLREIAPYLNKLMSTRAGIVLC"
     gene            complement(12365..13204)
                     /locus_tag="VGB76_19260"
     CDS             complement(12365..13204)
                     /locus_tag="VGB76_19260"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEY0101072.1"
                     /translation="MSALPSEQNHLEDIEIALLLEGLYRVYGFDFRDYSPASIKRRIL
                     ERMRAEKIATVSAYQDCVLHDDACMERLLVGLSVHVTSMFRDPSFYLTFRTKVVPILR
                     TYPTVRIWHAGCSTGEEVYSMAILLQEEGLYRKCVIYATDISREVLRKAREGIFPLSA
                     MQEYTGNYMRAGGKHEFSDYYTAHYDNVIFHPALKTNVVFSEHNLVTDGSFNEFHVIL
                     CRNVMIYFNKALQERVHNLVYDSLSMFGVFGLGNKESLKFTPRADFYERLDSRDKLYR
                     KVK"
     gene            complement(13207..>16944)
                     /locus_tag="VGB76_19265"
     CDS             complement(13207..>16944)
                     /locus_tag="VGB76_19265"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0101073.1"
                     /translation="SFASEVTRVAREVGTEGKLGGQADVRGVAGTWKDLTDNVNSMAG
                     NLTGQVRNIADVTTAVANGDLSKKITVDVKGEILELKNTINTMVDQLNSFASEVTRVA
                     REVGTEGKLGGQADVRGVAGTWKDLTDNVNSMASNLTGQVRGIAKVVTAVAGGDLKRK
                     LTVEAKGEIAELADTINSMTDTLATFADQVTTVAREVGVEGKLGGQANVPGAAGTWRD
                     LTDNVNGLAANLTTQVRAIADVSTAVTKGDLTRSIDVEALGEVAALSETINEMILNLK
                     ETTQKNTEQDWLKTNLARFTRMLQGQRDMATVSQLVLSELAPLVDAQQGVFYVNASNN
                     GSPLMKLLGGYAFTERKNLSNEYKPGQGLVGQCVLEKERILLTNVPDNYIRIGSGLGE
                     AAPLNIIVLPVLFEGEVKAVIELASFNRFNDTHLTFLDQLTESIGIVLNTIEANSRTE
                     DLLLQSQSLAAELQSQQDELKKKNEQLEQQAESLRESEELLKSQQEELQQTNEELQEK
                     ATLLEKQKAEVEAKNREVEEARWAMEEKAEQLALTSKYKSEFLANMSHELRTPLNSML
                     ILSRQLSENKDDNLTPKQVQFAETIHSSGADLLSLINDILDLSKIESGMMGIDISDVS
                     FTEVGGQLERSFQQVALDKRLGFSIERDDKLPVFMRTDEKRLQQILKNLLSNAFKFTE
                     QGSVTLRIEPALPGMKFYQEALNRVERVVAFSVSDTGIGIAADKQRIIFEAFQQADGT
                     TSRKYGGTGLGLSISREIARLLGGEIRVVSVPGEGSTFTLYLPLSYVSVATSGNGSGR
                     TLDASGGTRPAQAATVAEPISAWTSTAISDMEAALIATTEISDDRNDIFDGERVVLIV
                     EDDLNFARILLDLAREKNFKGLVATRGDAALALARKYKPDAITLDIKLPDRDGWTVLD
                     RLKHDPNTSHIPVHIISGEEQRQRALHLGAITHLQKPVSREDLASAFDQITAFADRRV
                     RRLLVVEDDDVQRMSIVELIGNGDVTTTAVATGQEALDALQAEPFDCMVVDLKLPDMS
                     GFELIEKIQKDLGRADLPIIVYTGKELTGKEETQLRKVADAIIVKEVSSPERLLAETA
                     LFLHRVEANLPEPKRRMLEQLHRRDPVLAGRRVLIVDDDVRNIFALTSALESHNMEVV
                     HAENGQEGIDTLQATEGVEAVLMDIMMPGMDGYQAIQAIRQIERFKNLPIIALTAKAM
                     KADRDRCIEAGASDYISKPLDIDQLLSLLRVWLYHQEEAQG"
BASE COUNT         3148 a         5395 c         5258 g         3143 t
ORIGIN      
        1 caagcgtttg cgggtcgcgc gggtggagtg ggcgaagacg agtttcgact cgtggtggtc
       61 tggcgcgagc gcgggcttca agtccgacgc ttcaaccgaa ggcttcgact atcaactcgc
      121 ggaaatcaac caactcgccg cagacgatac gtggacgccg accaaagctt tgcccgaggc
      181 caccggcaca gccgtctgga ccggcacgga gatgatcgtc tggggcggcg gcaaacagaa
      241 ttcaggcgcg cgctataacc ccgccacgga cacctggacg cccacccccg tcaccaacgc
      301 tccctctcat cgcagcggcc actcggcggt ctggacgggc acggagatga tcgtctgggg
      361 cggctgcgaa cgacacttcg acttctgcgg gacgaacgac ggaggccgct acaatcccgt
      421 ctcggacaca tggacgcgca cgagcctcgc caacgctccg acgccgcgtt cgcgccacgt
      481 cgccatctgg actggctcga agatgattat ctgggcgggg cttcaccacg gttataacaa
      541 tggcggcagg ccgatcaaca gcggcggcgt ctacgatccc gccagcgatt cgtggacgac
      601 gatgaacacg acgaacgcgc ccgaaaacgg cggcagcggc ttcaaggcgg tctggacggg
      661 ttcggagatg atcgtctggg gcggcaatac gagcgtcgga ggtcgttata atccgaacac
      721 aaacagctgg acgccgacga atcctctgaa cgcgccaagc cagcgcgcag accacactgc
      781 catctggacg ggcacggaga tgatcgtctg gggcggaacc tccggcatcg agccgctcaa
      841 cacgggcgga cgctacaacc ctgcaaacga ttcatggacg ccaacctcta cggcgggcgc
      901 gcccagcccg cgctatcttc acacggcggt ctggacgggc acggagatga tcgtctgggg
      961 cggcagtgcc cccctgccgg tcaacacggg aggccgttac aatccttcgt cggattcatg
     1021 gactccgacc aacaacgcga acgcgcctcc gcccaagtca ggccacgtcg ccgtctggac
     1081 cggcacggag atgatcgtct ggtctgtcac ggagaacaaa accggcggac gctacaaccc
     1141 tgcgaacgat tcatggacgc cgacggacaa taacgattcg ccgccgccgg ttaatttcgg
     1201 cgtgtggagc ggcgcggaga tgattatctg gagtccgacc tccgcctgcc tgagcggctg
     1261 cccggctgcg ggcaaacgct acgacccggc gacctatacg tggcgaccaa tgagcaacgt
     1321 cggtgcgccc atcccgccgc ccgcagaccg gcctatcgta gcggtctgga ccggcacgga
     1381 gatgatcgtc tggggcgcga acgacggcca gtacacgacg cccggcgagg gcggtcgcta
     1441 taaccctcaa accaattcgt ggcaacccgt cacgacgagc ggcgcgcccg tcaaccgtgc
     1501 ttacttcgca ggcgtctgga cgggcacgga gatggtcgtc tggggcgggc aaagcaacga
     1561 gggcacgatg cagaacacgg gcggtcgtta caatccttcg accaacactt ggaggccgac
     1621 cagcaccgca ggcgcacccg aagcgcgcta tatccattcc gccgtctgga cgggttcgga
     1681 gatgatcgtc tggggcggct cagccgggtc gctggctcat ctcaacacgg gcggtcgcta
     1741 caatcctgcg aacgatacct ggacgccgac gacgacgagc ggcgcacccg aagcgcgacg
     1801 ttttcacacc gccgtctgga cgggcgcgga gatgatcatc tggggcggtc gcatcggcga
     1861 ttacatcagc agcgacggac tcttcaacac gggcggtcgc tacaatccct cgaccgacag
     1921 ttggacgccg acttcgacga cgggtgcgcc cgctcctcgt ttcaaacata cggcggtctg
     1981 gacgggcgag ttgatgatcg tctggggcgg cattgcgacg aacgaactgg gttatgccct
     2041 cagtacgaac acgggcggtc gttatcaacc cggcacggac acgtggacgc cgacgagcac
     2101 gctgcgcgcg ccgggtcgtc gccacgacca cgtcgcgctc tggacaggca cagggatgct
     2161 cgtctggggc ggctatgcgg aggatggctc ggccacgcac ggcggctact actttgcgtc
     2221 cggcgaagcg aacggcaaca cgccgcccac cgtgcgcatc actgcgccgt ccgacgacgc
     2281 gaccttcgag gcgggcgcga acgtacagat caccaccgag acgggcgacg cggacggcac
     2341 ggtcgcctcg gtccggttct acgccaacga cacgctgctc ggctccgatt cgactgcgcc
     2401 gttcgactac agttggtcgg gcgtgcccgc cgggagctac accgtcaagg cggtggcgac
     2461 ggacgacagc ggcggcgcaa ccacctccgc cccggtttct ttcaaggcaa tagtcccggc
     2521 ggcgaccgcg aatctctctc tcggcgtcgt cgattcgcca gaccctatca ccgtgggcgg
     2581 caatctgact tacgcgctca cggttaataa cggcagtcag gccggcgcgt cgaacgtcgt
     2641 cgtcacggcg acgcttcccg cgaacgccgc tttcgtctcg gccacgaacg gttgcactcg
     2701 cagcggcaac actgtggcct gtgcgctcgg ccaactcgac cagggcgcga accgcgcgct
     2761 cagtatcgtc gtcacgccga acgtcgcagg cacgctcaac ttcgccgcga gcgtcgcttc
     2821 cgacacgggc gaccccgacg cgtcgaacaa cgcggcgcag gccgccacgg acgttcacct
     2881 gcgcggcacg gatgtcaaaa tgggcgacgt gctgatcagc gaattccgcg cgcacggcag
     2941 cgcgggcgta gatgccgaac tcgatgattt cgtcgaactc tacaacaaca ccaacgtagc
     3001 gatcacggtt ggtcccgtag acgcctccgg cggttgggcg ctcgtcgcgc gcgaggctaa
     3061 tggtctgccc gtcgtgcgtg ccaccgtccc gaagggcaca gtgattccgg cgcgcggcca
     3121 ctacctcgtc accgccgccg cctactcgct cggcgcgctc gccgcaggcg acctgcccgt
     3181 cgccgggaac attctcgaca cgggcggcgt cgcactcttc cgcactgccg acccggcgaa
     3241 gttcggtgcg atggaggaac gcctcgacgc cgcaggcttc aattccgtga gcgaccagat
     3301 gtaccgcgaa ggttccggac tcacgccgtt tgacgccgtg caggccgagc aatcgttcgt
     3361 gcgcaagctg aacagcgtcg ggcagccgca ggacacgggc gacaacgcag ccgactttca
     3421 gttgatctcg aaaacccccg gctcgttcgg cgcggtgccg gctatcctcg gcacgccggg
     3481 accggaaaac acgggcagcc ccatccagcg caacgcgtcc gtcaagcctt cgctcgttga
     3541 tccggggtgc gcggggttcg gcgcggcgac gagcggctgc gcacgcgtgc gcgaaggcgg
     3601 aaccgtggcg aacggcgcgt tcggcacact cacgatccgc cgccgcttca ccaacaagac
     3661 gggcgcggcg atcaacaggc tccgcttccg catcaccgac gtaacgacgc tcggcacgcc
     3721 cggcacgaac gcgctcgcag acctgcgcgc gatctcctcg gtcgatgtag aagcgacggt
     3781 gaacaacaac cccgtcatca tcaagggcac gctgctcgaa tcaactcaca cgctcggcgg
     3841 cggtgtgaac acgacgctca ccatcccgct cgcgggcaat tcgctcgcgc cgaacgcctc
     3901 cattgacgtg caatttcgtc tcggcgtcga gcgcaacggc gcgttccgct tcttcgtcaa
     3961 cgtcgaagca ctgaccacgg ctctgcttga ggagggcgcg ggcgcgaaaa gcggcagccc
     4021 gaccaaacag aaccccgaca aatagagcgg caaacagagc ggcggcaaac cgcacggctc
     4081 agacaggacg acggcggcgt tggcgtggac gagaaatcgt ttgctcaacg ccgcctttgt
     4141 cgtgttaagt ttagccttaa aaatatcccg taaagttgta aggagagatt cgatgagata
     4201 ccggatgatg ctgacttcca gcgtgctgct ctcggcctta tgcttcaact ctctcgccct
     4261 tcctgcttcc gccgtcgcac gccagaagcg acgcgcgccg aaggccgcag cggtgtcaac
     4321 tgctcgcacg tcgctcgacg ccaagattcg tcgcttcgcc ccgaccacgc tcacagccga
     4381 cacatcgcgc ctctcggcgg gcgaccgccg cgcgctcgcc agaatcatcg aggccgcgaa
     4441 gctgttagac ccgctctatc tacgtcaggt gtggagcggc aaccctgcgc tcaagcggcg
     4501 gctcgaagcg gaccagagcg cggaggggcg tgcgcgcctg cactacttca acatcaacta
     4561 cggcccctgg tcgcgcctcg acgacaacga gccgttcgtc gaaggcgtcc cgcacgagaa
     4621 gccgaagcat gccaactact accccgacga catgacgaag gaagagttca acgcgtgggt
     4681 gcagacgctc aaggcggaag agaaggcgcg cgccacgggc ttcttccacc tcattcgccg
     4741 cgacgcgaac ggcaagctca agaccgtgcc ctacagcgtc gaataccgcg agtttctcga
     4801 acccgccgca cgcctcctgc gccaagcggc gcgagagacg accaacgcga cgctcaaagc
     4861 gtttctcgcc aaacgcgccg acgcttttct ctccgacaac tattacgaaa gcgatgtcgc
     4921 gtggatggat ttggacgcgc cgcttgacgt gaccatcggc ccttacgaaa cttacgaaga
     4981 cgagttgttc agctacaagg ccgccttcga gtcctacgtc acgctgcgcg acgaagcgga
     5041 atcggccaag ctcgcaaagt tcagcggtta cttgcaggag ttggaagaca acctgccgat
     5101 ggaggcgcgt tatcgcaatc cgaaactcgg cgcggcctct cccattcgcg tcgtcaatga
     5161 actcttctct tcgggcgagg gcaggcgcgg cgtgcagacg gcggccttca atttaccgaa
     5221 cgacgagcgc gtcgtccgtg aaaagggttc gaagcgcgtg atgctcaaga acgtgcagga
     5281 ggcgaagttc aataagacgc tcctgcccat ctcgcgcgtc gtgctcacgc ccgcaggcca
     5341 acgcgcgctc tcctttgaat ccttcttcac acacatcctc gcgcacgagt tgatgcacgg
     5401 gctgggaccg cacaacatca aagcgggcgg cggggagacg accgtgcgca tgcaactcaa
     5461 ggaactctat tccgcgattg aagaggcgaa ggccgacatc accggcctct gggcgttgca
     5521 gtatttgata gacaaaggac tggtggagaa acgcatggag cgttcgctct atacaacctt
     5581 cctcgcgagt tgttttcgct cggtgcgttt cggcatcacg gaggcgcacg gcaaaggcat
     5641 cgccttgcag ttcaactacc tcacggacga gggcgcgatc aaattcaacg agcgcgcggg
     5701 cactttcgag atcgacgagt cgcgcatcaa agccgccgtc gcgaagctga cgcgcgagat
     5761 tctcacgctg caagccgaag gctcttacga gcgtgccaaa gcgttgctcg acaaatacgg
     5821 cgtgattcgc ccgcccatgc agcgcgccct cgcacgtctc ggcgatgtgc ccgtagacat
     5881 cgagccgcac ttcccgctcg ccccagggta agagaggtaa taagagaagt aagttgagat
     5941 tattcgacgg gcgcgggttg aggctgagac ggcggcgaag gcggagcgtc gttgtcggtc
     6001 gcgcgataac tttccaacgc gtattgaacc acgcgctgct cgcccgtcga gttgcaggtg
     6061 atgtagttgt gaaactccga ggtgcgtgcg gcgacgtgcg cttcgtgaat tttgcccgcg
     6121 cccgcctcgg agaatttcac cagccagctt ttttccccgg tgcgttcgtg cctgcggtag
     6181 cgcagttcgc cgatggcgtt gatgcccgtc tccctgcgta tgaagaactc agcggcctgc
     6241 acgggatacg agtaacaggt gcgcccgcga tagcccgcga gcacgagtcg ccgcccgtgc
     6301 tcatactccc gcaggatcgt ccgcccggcc tcttccgtga cgtgcgcgta aaagaggccg
     6361 tgcgggaagc agatgagatt ggccgcgaac ctgtcgccgc cgacgtgcga cgactgccag
     6421 acccgctcgc gttccgccgt cgcaccttcg cgcagagact tatagagcgc atagccgaac
     6481 ttggcgcagc acttgtcgcg cttgccgtgc gtgcagacga gatagagcgg ctcgtccgtg
     6541 cgaactccgc ccgcgagaga gtcgcctgcg gcggcgttgg cggcggcgag atcaaggttc
     6601 aacaactcct cgtgactgga aaaagtgaac cgcacgattg aaggcgagcc ttcgcggcaa
     6661 cgcacgatga aacatgagag cgcatcgcgg cgcagccgtt cctgtttgat gaacagcagg
     6721 cgggagcgtg gaacgagttt gcagaagttg ccgagatgcc ttttgacctg cgccgagagc
     6781 gtgctctctt cgaacgcgtg cgcgccccac ccctctcgat attcgacgag cagccacaca
     6841 tcgccaatcg aagccgtgcc gaaacttcct tcggcggcct cgcgcgacaa atcggaacaa
     6901 tagaagaagg gtcggctcat cgtttccgga agcaggctca cgtcaacatc tccgcacgct
     6961 taatctttgg cgacggtgcg gacgagtccg tgccgcagca actcgccgag aaaggcgcgc
     7021 acggtctgtt cgtcaagtcg ccctcccgcc gcctcgcata attcggagac ggagagcgca
     7081 gcggtgcgcg cgtccgccaa tcgcctgagg acgggcaacg cacccgccgc gaaccgccag
     7141 cgttttttgt gacaacaaaa ctccaccacc ccgtcaacga cctgcaaatc gagcgggcgc
     7201 ggcgcgacga ggcgcacgcg tacatcctga tcggcaggga cgggctgcgt taacgcgctc
     7261 caaggcaggc tgacctgtgc gcgcggctcg gcctgcgcgt cctgttcttt aaagtatcgt
     7321 tcgagcaatc cagcatccca cgcggcgcgc agttcttcgc cgagtcgctc gacgtgggcg
     7381 gcgcgctcgg cgggcgtcgc aaagcgcggg aggtcttggc ggaaaagctc gctggcgcgc
     7441 agttgcccgg cgagccacgc gagcaggtcg atgcctgtgc gtttgtgtat gccgacggtg
     7501 agatggaggg tcggttcgtc gagcggttcg gcgacgtgcc accagccgcg cgggatgtag
     7561 agcaagtcgc cgtcttcgag cgtcgcctgc cagagaggct cgcctgaggg tttgggcaag
     7621 tcggcgtcgc cagagagcgg gtgcgggcgc gtctcgccgt agatgctcca acgcttgcgc
     7681 cccgtgacct gcaagataaa aacgtcgtga tcgtcccagt gcagatcaaa gccgcgcgag
     7741 gtgtgccacc cggcgtagga attgatctgc acgcgctctt ggaagaggag ttccaatccg
     7801 gcggccaacg cttcgagcgg cgcgtgtagt tcctcaaccg catcgagcac gagcgttgcg
     7861 cccgcgcgaa gctcggctgt gaatttgacg ggttgcagac gcgggatcgt ctgcttgctg
     7921 cgtgcgcccg aagtgtggcg caggtaggag gcgacgggga gggcacgccc ttcgcgcatc
     7981 aggcgcaggc gcgggaagtc gaggcgatgc tgccggagaa tctcattgag acgctcccag
     8041 ggcagcaacg acgcgaacct gcccgcccgc ccgcgaatat gcctgtacgt ctgaccccag
     8101 gaggaggcga ggaactcctc cgctgtgtgt ggctccaaaa gtctgcttaa gctgcgcgcg
     8161 acctcggcat tcataaaacg gccatcaaac gcttaagcat tcttgctgtc aatcgtgatc
     8221 accgatgcgt cagaatcgtc tgtgccgtcg gagtccgaag aatcgtccga gccgtcgtcc
     8281 gcgccgagcg agtcggtcaa atccgtgtcc gtgctcgtgc ccgtcgagtc taaactgtcg
     8341 gagccggtgg aatccgaact gtccgtgtcc gatgaatcgt cgcccgtgtc cgagccgccg
     8401 tcgttcgacg tgtcgctgct gtcgtcgccc gcgctcaagt gcgcgctggc ctgtaaaggc
     8461 tcaaccgata acagcagacc cgaagcatcc gtcgtctccg gctgctcaac ctgtcctgaa
     8521 ttgatctgtt ctgacatagc atctcctctt cgtattgaaa gtagttcgca gtgtgcgcac
     8581 agattattat gagccgcgtg aaactacaag gacgcgccgc acgcctcgcc cggatgggtg
     8641 cgcacgcgcc cgctcacctt ttcgtttatg ccgcaaccca tatcgtgagt tcgcgcccgg
     8701 cgggttcagg cgcgcggcgc ggcggcggtg tgagagtcgc ggggaaacag cggcatttac
     8761 agagatttcg cgcgggtgcg gtgagtctcg atttgtggca cagtccttgt tatagagaat
     8821 accgagtcgt caacacgcaa gtgaacgacg atccaattga gaagcccgcc agcgaagggg
     8881 agagcgctgg cgggcttctc cttctatgct cgcgccatcc cggcgacgcg cgtttgctct
     8941 ctggcgcggg ctgcctcata gtagttgggc aattgaaatc aaaacagtgg gacgcgagcg
     9001 cggggcgaca attcaatttc gcggcaattc agctacccca cccgacaagg aaccaaccgt
     9061 acacgcgatg ttgaaaaaga ttcgtctcgg cgacgacgtg cagtcctact gcggacgctg
     9121 caaagaagaa cgcatgcatc aggtcgtcgc cttacattcc gatggccgga tcgagcgcgt
     9181 tacctgtaac tactgtcaat ccacacatcg ctatcgcgac ccgcaggcca cggcggcggc
     9241 agcggtcaag actcgtcgca cgaccggcgc acgcgcctcc gaagccagag agcgcgcagt
     9301 ttccgacgcg cccgcgcgcc cctattcgcc gcaagaaagt tatgcggcgg gcgagcgcat
     9361 cacacatccg aaatttggag agggcagcat tgttgaagcg cggcagggca agatcgatgt
     9421 ccgcttcggc aaggagttgc gcacgctttt gcacgcgggc tgaaactctc gcgggcgacg
     9481 ctggcgcgca ccccgacgcg agacgcccgc cacttcgcat cacgaacgca gcgcgcgaaa
     9541 cgactcaggc atccacgggc gtttcgcgcc cggccaggcg cgccagcgtc gagatcaggg
     9601 ctttcgggtc aaccggcttt gcgagatgca tctgaaagcc tgcggcgagc gcgcgtttgc
     9661 tgtcctcaac cccggcgtag gctgtgagtg cgaccgccgg aatctgcccg cgcgcgccat
     9721 caccgtagag tttgcgcacg cgcctgatca actcataacc gtcttcgaac ggcatgccga
     9781 tgtccgagac gagcagatcg ggttgccact gtgcgagcgt ccgcaaggct tccgccacag
     9841 acccgctcgt ctgcacttcc gctccctgtc gcccgagcac ctcctgcacc aattcgcgcg
     9901 tgtccgcttc gtcgtccacg acgagaatac gcaagccttc gagcaccgcc gatttggcga
     9961 gcgacccgtg cgcttccact tccgtcgccg catcaggcgc gggcgcgccg ttgccgctcg
    10021 cctgcgcgcc cgaagcgagc ggcaggctga gcgtgaaagt tgccccttgc ccgtcgccgt
    10081 cgctcacggc gcgaatcgct ccgccgtgga gttcgacgag atggcggacg atggcgaggc
    10141 cgaggccgag gccgccgtgc ttgcgcgtcg tcgtgccgtc ggcctgccgg aagcggtcga
    10201 agatgtaggg cagaaactcc ggcgcaatgc cctgccccgt atcgcgcacc tccaactcga
    10261 cgtgcctctc gtcgcacttc aggccgacct cgacatggcc gccttccggc gtaaacttca
    10321 ccgcgttcga gaagagattc cagaccacct gctgcaagcg cgccggatcg cccgacacca
    10381 tgcagtgaga tgtcttaagc tgcggcttga cgtggatgga cttggcttcc gccatcgggc
    10441 gcacggtgtc aatcgtggct tcgatgatcg cggagagttc gacgggatga aagtcgagcg
    10501 cgagtttgcc cgtgatgatg cgcgacacgt cgagcaggtc gtctatcaac tgcgactgcg
    10561 agcgcgcatt gcgttcgatg gtttcgagcg cgcgagccat catcgacatg tcgaggttgc
    10621 cgccgcgtat gagatgggtc cagccgagaa tcgccgtgag cggcgtgcgc agttcatgcg
    10681 agagcgtggc taagaattcg tccttcgtgc ggttggcgcg ttcggcttcg gcgcgtgcgg
    10741 cctgttcacg aatcaggagg cgtgcgcgtt cctcttcggc ctgtttgcgc tcggtcactt
    10801 cctgctgcaa ctccgtggcg tggcgttgcg cctccgtgta gagccgcgcg ttctcgtaag
    10861 aggcggagac ttgcgtggca agcgtcacgg cgagccgctc gtcggcttcg ctgaactcct
    10921 ccgcgtcgag tttgttggtc aggtagagcc agccgtaggc gcgcgcgggc gacaggatcg
    10981 gcgcgcctaa gaatgactgc atcgagacgt gcggcgacga gagatgttcg gcttcgagcg
    11041 tctgcgccac ttcgctcaaa cgcagcgggc ggccttcggc gatcaactgt ccgagcacgc
    11101 tctcgctcac ctgcggcgcg ttcacgttgt cgtcgccgcg ctcgcacacg tcgcagtggc
    11161 aatagtagcg aagcgaaccc ccgtgctcgt ccagcacgcc gacgcccgcg tgctccgcgc
    11221 ccacgatctc gcgcgccgcg ctgcaaaact tctccaagac ctgttcgggg tcgcgttcgg
    11281 cggcgagttc ctgtccgagg tcgatcaatt tgccgagacg ttggagattg gcgttctcca
    11341 gttcgatatt tttctcgtgg agaagcgcgg cctgccgctt gatctcttcc gtcttcttat
    11401 acaactcgac gaagacggcg accttcgagc gcaggatgtc cggatcgacg ggtttgagaa
    11461 tgtagtccac cgcgccgagc gagtagccgc gtgcgatatg ccggttgccg gtcgagtctg
    11521 cggtgaggaa gatgatcggg atgtcgcggg atttttcgcg cccgcgaatg agcgcggcgg
    11581 tttccagacc gtcaatgccc ggcatgtaga catcgagcag gatgacggcc acctcgcctt
    11641 cgagcagata acgcagagcc tcgtcgccgg aagcggcgcg gacgagattg cggtccggcg
    11701 cttgcaggat ggcttcgagc gcgagcaggt tcgtcgggct gtcgtccacc atcaagatat
    11761 tgattttttg cgtctcagct agcataggac gattccggct cgtgttgaca ttagtttatt
    11821 caagtagggc gcgatctctc tgagcggcag gatcgcgtca accatcgtgg cggcaatggc
    11881 cgcctgcggc atcccgcgcg actccgcgct cgacgggtct tccaccagac acaggccgcc
    11941 gtaggatttt atcttcgcga gtccacgcgc gccgtccgtg ttggctcccg tcaggatcag
    12001 gccgagcgtg cgctcctggt aaatgtccgc cgccgattca aacagcacgt tgaccgaggg
    12061 gcgcgcgtaa ccgacagggg cttccgtgga gagagcgaag ccgtcgcgtt cgatgagcag
    12121 atgatagtcg cgcggcgcga gatagacgcg ccccggcgca atcgcctctt tatcgttcgg
    12181 ctccgagagc gggagcgcgc agtacttttg caaaaactcg cagaggttat cagaatcttt
    12241 gccgcgatgc tgcacgacga cgatgggcag cgggaaatca cgcgccagcg cgggcagcag
    12301 aatttgcagc gcagccagtc cgccgtatga agtgccgacg acgacgagtt ctacggtcaa
    12361 cctcttattt cacctttcgg tacagcttat cgcggctgtc cagcctctca taaaaatctg
    12421 cgcgcggcgt aaacttcaga gactctttat tgccgagtcc gaagacgccg aacatcgaga
    12481 ggctatcgta aaccagattg tgcacgcgct cttgcagcgc cttgttgaaa tagatcatca
    12541 cgttgcggca caggataacg tgaaactcgt tgaacgatcc gtccgtcacg aggttgtgtt
    12601 cggagaagac cacgttggtc ttgagcgcgg gatgaaagat cacgttgtcg taatgcgccg
    12661 tgtagtagtc cgaaaactcg tgcttgccgc ccgcacgcat atagttgccg gtatattcct
    12721 gcatcgccga gagcgggaag atgccctcgc gcgccttgcg taagacctcg cggctgatgt
    12781 ccgtcgcata gatcacgcac ttccggtaca agccctcctc ctgcaagagg atcgccatcg
    12841 aatagacttc ttcgcccgtc gaacagcccg cgtgccagat gcgcacggtc gggtaagttc
    12901 gcaggatggg cacgaccttc gtgcgaaacg tcaggtagaa actcggatcg cgaaacatcg
    12961 acgtgacgtg cacggagagt cccacgagca gcctctccat gcacgcgtcg tcgtgcaaca
    13021 cgcaatcctg ataggccgag acggtggcga tcttctcggc gcgcatgcgt tcgagaatcc
    13081 gccgcttgat cgaagcaggc gagtagtcgc gaaagtcgaa accgtagacg cgatacaagc
    13141 cttcgagcag gagggcgatc tcgatgtctt cgaggtggtt ctgttcagac ggcaatgcgc
    13201 tcatctttat ccttgcgctt cttcctgatg atagagccag acgcgcagca gcgacagcag
    13261 ttgatcgatg tccagaggct tcgagatgta atcggacgcc ccggcctcga tgcagcggtc
    13321 gcggtcggcc ttcatcgcct tcgcggtcaa agcgatgatc ggcagattct taaaccgctc
    13381 gatctgccgg atggcctgaa tggcctgata accgtccatg cccggcatca tgatgtccat
    13441 caagaccgct tcgacgccct cggtcgcctg caaagtgtcg atgccctcct gcccgttctc
    13501 agcgtggacg acctccatgt tgtggctctc aagcgcgctc gtgagcgcga agatgttgcg
    13561 cacgtcgtcg tcgacgatca agaccctgcg ccccgcgagc acgggatcgc ggcggtgcag
    13621 ttgttccagc atccgacgct ttggctccgg tagattggct tcgacgcggt gcaggaagag
    13681 cgccgtctcc gccaacaggc gttccggcga actcacctcc ttgacgatga tcgcatcggc
    13741 gaccttgcgc agttgcgtct cctccttgcc cgtcaactcc ttgcccgtat agacgatgat
    13801 gggcaagtct gcccgaccca gatccttctg aatcttctcg atcaactcga agccggacat
    13861 gtccggtagt ttgaggtcaa ccaccatgca atcgaagggt tcggcttgca gagcgtcgag
    13921 ggcttcctgt ccggtggcga cggcggtggt cgtcacgtcg ccgttgccga tcaactcgac
    13981 gatgctcatg cgctgcacgt cgtcgtcttc cacgacgagc aggcggcgca cgcgcctgtc
    14041 ggcgaaggcc gtgatctgat cgaaggccga ggccaaatct tcgcggctca cgggtttttg
    14101 caggtgcgtg atcgcgccga gatgcaaagc gcgctggcgt tgctcctcgc ccgaaatgat
    14161 gtggacgggg atgtggctcg tgttcggatc atgcttcagg cgatccaaca cagtccagcc
    14221 gtcgcggtct ggcaacttga tgtcgagcgt gatggcgtcc ggcttgtatt tgcgtgcgag
    14281 cgcgagcgca gcatcgccgc gcgtggcgac gagtcccttg aagttcttct cgcgcgcgag
    14341 gtctaacagg atgcgcgcga aattgagatc atcttcaacg atgagcacga cgcgctcgcc
    14401 gtcgaagatg tcgttgcggt cgtcgctgat ctcggtggtg gcaatgagcg cggcttccat
    14461 gtcggagatg gccgtcgaag tccacgccga gatgggttcc gcaacggtcg ccgcctgcgc
    14521 ggggcgcgtc cccccggagg cgtccagagt gcggcctgag ccgttgccgg aggtggcgac
    14581 ggaaacgtaa ctgagcggca ggtagagcgt gaaggtgctg ccctcgcccg gcacactcac
    14641 gacgcgaatc tcgccgccga ggaggcgcgc gatctcgcgg ctgatggaga ggccgagtcc
    14701 cgtgccgcca tacttgcggc tcgtcgtgcc gtcggcctgc tggaacgcct cgaagatgat
    14761 gcgctgcttg tccgccgcga tgccgatccc cgtgtcgctc acagagaagg cgaccacgcg
    14821 ctcgacgcgg ttcagagcct cctggtaaaa cttcatgccg gggagcgcgg gttcgatgcg
    14881 cagcgtgacg cttccctgct ccgtgaactt gaaggcgttg gagagcaggt tcttgagaat
    14941 ctgctgcaaa cgcttctcat ccgtgcgcat gaagacgggc aacttatcgt cgcgttcgat
    15001 ggagaaaccg agtctcttgt cgagcgcaac ctgttggaag ctgcgttcga gttgcccgcc
    15061 cacttccgta aacgagacat cgctgatgtc tatgcccatc atgccggact cgatcttgga
    15121 gagatcgaga atgtcgttga tgagcgagag caggtctgcg ccggacgaat ggatcgtctc
    15181 ggcaaactgc acctgcttcg gcgtcagatt gtcgtccttg ttctcggaca actggcgcga
    15241 gaggatgagc atgctgttca gaggcgtgcg cagttcgtgc gacatgttgg cgagaaactc
    15301 cgacttgtac ttggaagtga gcgcgagttg ttcggccttc tcttccatcg cccagcgcgc
    15361 ttcctccacc tcgcggttct tcgcctcgac ctcggccttc tgcttctcca gcagcgtcgc
    15421 cttctcctgc aactcttcgt tcgtctgctg caactcctcc tgctggctct tcaacagttc
    15481 ttccgattcg cgcaaagatt ccgcctgctg ttcgagttgt tcgttcttct tcttgagttc
    15541 gtcctgctgg ctctggagtt ccgccgcgag cgattgcgat tgcagcagca ggtcttcggt
    15601 gcggctgttg gcctcaatcg tgttcaggac gatgccgatg gattcggtca actgatcgag
    15661 gaacgtcagg tgcgtatcgt tgaagcgatt gaacgacgcc agttcgatga ccgcttttac
    15721 ttcgccttca aacaggacgg ggagcacgat gatattcaag ggtgcggctt cgcctagacc
    15781 cgacccgatg cggatgtagt tgtccggcac gttcgtcaaa aggatgcgct ctttttccag
    15841 cacgcactgg ccgacgagtc cctgccccgg cttgtactcg ttcgatagat tcttgcgctc
    15901 cgtgaaggcg tagccgccga gcaacttcat taggggcgag ccgttgttgg aagcgttgac
    15961 gtagaagacg ccctgctgcg catccacgag cggcgcgagt tcggagagga cgagttggga
    16021 gacggtcgcc atgtcgcgct gcccttgcag catgcgtgtg aagcgcgcga ggttcgtctt
    16081 caaccagtcc tgttcggtgt tcttctgcgt cgtctccttg agattgagaa tcatctcgtt
    16141 gatggtttcg gagagcgcgg ccacttcgcc gagcgcctcg acatcgatgg agcgcgtgag
    16201 gtcgcccttc gtcacggcgg tggaaacgtc cgcgatggcg cgcacctgcg tcgtgaggtt
    16261 ggcggccagc ccgttcacgt tgtcggtcag gtcgcgccag gtgcccgccg cgcccggcac
    16321 gttggcctgc ccgccgagtt ttccttccac gcctacttcg cgcgccacgg tcgtcacctg
    16381 atcggcgaag gtggcgagcg tgtcggtcat gctgttgatg gtgtcggcca actcggcgat
    16441 ctcgcctttg gcctcgacgg tcagcttgcg tttcaagtcg ccgcccgcca ccgccgtcac
    16501 gaccttcgcg atgccgcgca cctgacccgt caggttgctc gccatcgagt tcacattgtc
    16561 ggtgaggtct ttccaagtac ccgccacgcc gcgcacatcc gcctgcccgc ctaactttcc
    16621 ttccgtaccc acttcgcgcg ccacgcgcgt cacttccgac gcaaacgaat tgagttgatc
    16681 caccatcgtg ttgatggtgt tcttcaactc aaggatttcg cctttcacat ccaccgtgat
    16741 cttcttcgac aggtcgccat tcgctacggc ggtcgtcacg tccgcgatgt tgcgcacctg
    16801 acccgtcagg tttccggcca tcgagttcac attgtcggtg aggtctttcc aagtaccggc
    16861 cacgccgcgc acgtccgctt gcccgcccaa ctttccttcc gtacccacct cgcgcgccac
    16921 gcgcgtcact tccgacgcaa acga
//
LOCUS       DASWNS010000558        20449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_151876, whole genome shotgun sequence.
ACCESSION   DASWNS010000558 DASWNS010000000
VERSION     DASWNS010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20449
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151876"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VGB76_19270"
     CDS             complement(<1..578)
                     /locus_tag="VGB76_19270"
                     /inference="COORDINATES: protein motif:HMM:NF023605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="HEY0101074.1"
                     /translation="MPKQPLAEVFGFPIDNQSPEATRYQEKRLCPFNNKVPNCTKDKA
                     NAPLGVCSVHESDAVAITCPVRFREDWLIADDAAAFFFPPATTWTSLVEVRLKDKYGQ
                     SAGNIDVVLVSYDDKGRVIDFGSCEVQSVYISGNVRAPFEHYMQDPANRSHMDWTGKP
                     YYPRPDYLSSSRKRLAPQLLFKGGILHTWRKK"
     gene            1150..1488
                     /locus_tag="VGB76_19275"
     CDS             1150..1488
                     /locus_tag="VGB76_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101075.1"
                     /translation="MRKDNSTRTPAARRREPSFEEPIRRWANLPITQDRARELVDLIT
                     RREDRDALDALMELICGVMPYAPSDDKHDLDERVLAGHAALWHAFADTTRAHNDLGAY
                     VVGLKCAEWE"
     gene            complement(1501..2736)
                     /locus_tag="VGB76_19280"
     CDS             complement(1501..2736)
                     /locus_tag="VGB76_19280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0101076.1"
                     /translation="MSESLTKDYDVIVVGGGPAGASAAVHLATRGARVLLVEREKFPR
                     AKLCGEFISPECLPHFARLGVLEEMSGAGGAQVGETLFFAQSGRRAGVPSAWFGKEAA
                     GALGLSRAEMDERLLQRARAVGVEVLEEAQVTGLLFADAARRTVCGVRVESRRGMSEL
                     RAGVTIDATGRARALARRVEPRVKVSGGATREAKRGGAASRRMPLVAFKTHLENAGGA
                     AGACEIYFYRGGYGGLSCVENELSNLCFIVAAQDVRARASDAARVMREIVMSNSRAAE
                     TLRGARVRSEWLAVGLESFGRREVVPAEGLLMVGDAASFIDPFTGSGMLMALESGELA
                     GEAVTSALPQLRAGAGFARLAEDYRARYRERFAARLRVCSWLRRVAFAPQFATEAAIA
                     ALGASERMRRVVARATRHA"
     gene            complement(2755..3450)
                     /locus_tag="VGB76_19285"
     CDS             complement(2755..3450)
                     /locus_tag="VGB76_19285"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0101077.1"
                     /translation="MFSERSYELEHLDKGDYTPEEYEGCLVELRRVNKWLGDARALKT
                     SLVPEIVESGVQSFSLLDVGAGSGELLRVVAREARRRGWRARLTGLELNERSARAILE
                     ESHDFEEIRAVRGDALRLPFADDSFDYVICSLFTHHFRDEAVVEILREFKRVARRRLF
                     VIDLHRHALAYFLYTTVGRLFLHNRLLREDGALSIRRSFRAGELRRMAETAQLENVEV
                     RRRFPFRLVLGGS"
     gene            complement(3657..4922)
                     /locus_tag="VGB76_19290"
     CDS             complement(3657..4922)
                     /locus_tag="VGB76_19290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY0101078.1"
                     /translation="MLRTLKQLRRVVITGMGCVTPIGIGREAFWRALVAGESGARRIE
                     SFDVSNSAVQIAAEVRAFDWEAQVGAKDRKHVPRTVPLALAAAREALEDAGLHFSDAS
                     LAERRATGVVLGTGGGGLAFTEQQYKYYFSGATTKASVYTIPASTHGGLSSELSMAFG
                     LRGLSHIISTGCTSSTDAIAYAAEHIVLGRQEVMLAGGVDAPIAPGILAGFNLMTVLT
                     KEWNDAPARASRPFSRDRSGIVLGEGAWIYVLEDYERARARGAKVYAEIKGYGATCDA
                     YHRVRLEETGEEPARAMRLALTDAGRAPSEVDYVNLHGTSTQLNDRIETQALKLAFGA
                     DAHAIPMSATKSQIGHPQGASGAAGLAAALCAINTKMIPPTINLDEPDPVCDLDYVPN
                     VAREADVRVALCNCIGFGSKNSALVVEKV"
     gene            5156..5626
                     /locus_tag="VGB76_19295"
     CDS             5156..5626
                     /locus_tag="VGB76_19295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0101079.1"
                     /translation="MDGSTKHIPEGYHAITPYLHVRDAARAIEFYKDVFGATEVLRMD
                     EPGGKIGHAEIRIGGSHVMLADEFPEMDVRSPESYGGTPVSLLLYVEDVDALAERAVA
                     AGAKLLSPLEDKFYGDRMGKLQDPFGHIWAIATHKEDVSPEELQKRATAAGLHG"
     gene            complement(5852..8692)
                     /locus_tag="VGB76_19300"
     CDS             complement(5852..8692)
                     /locus_tag="VGB76_19300"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY0101080.1"
                     /translation="MRRLFIRLIALACLFTLSTGGVSYVAPVSFVTRAQVRTTYNEGA
                     VGLGLLLRRLQTTASVLHTGAHPDDEDSALIARLARGDGARVGYLSLNRGEGGQNRIG
                     AELFDALGVIRTEELLQARKLDGGEQFFTRAFDFGFTKTREETAERWNERELLADMVR
                     VIRRFRPLVIISRWNGTPADGHGQHQFAGYLTPLAFAAAADPAAFPEQLAEGLRPWQA
                     KKLYVSVFGQPPAAAGASTGQGTNASVVEIDTGRFDPLLGRSFYEVAMEGRSQHKSQA
                     EAALELRGAQTSRVRLVKSAPSPFQVAQNERSIYEGLDTTLHGLIRSAGLRDDALSKE
                     LEQVDRAAKHALEHFNALAPRTIHAALAAGLLHTRQARRTLAQLKDSPAARAEADFLL
                     AQKEREFSEALRLAHGVRLDALADAETIARGGSFVVAVRVFADDPRVGRVEELRLRAP
                     EKWRVERLAEPSKGETAFVAREAALAVAYFRVHVPVDAELTQPYWLASSGREPRGRRD
                     MYVWDKDDPKTEPFAPPPVSAEVVMDVGGSPQTISAPVQYRYLDNVRGEVWREVNVVP
                     EINVQLDPALVIVPQGSRERTRQLTLQLVNNVEPALGQTESLLATGGAGISLPVGWRL
                     PETTPFNFNSEGERATQSYTFTVPRGTRAGEYYVRSFAATFLPERRLRQPHLPQSLTF
                     GQTSHAISYPHIQTHYYYRSAETIVRVFDLNVASVRVGYIMGSGDEVPEAIRRMGLTV
                     TLLDERQLAAGDLSRFDTIVVGVRASQTRPDFVAHHARLSEFVRRGGALIVQYQRPDY
                     AAKKLPPLPAAMEVAPQRAGGSATIARVVDENAPVRVLQPAHPAFNFPNRIDDADWQG
                     WVQERSLYNFTTFDAAYTPLLESHDAGEGEQRGGQVYARIGRGHYVYTSYAWFRQLPA
                     GVPGAYRLFANLLSLPKAPAPATRTAARRARD"
     gene            complement(8806..10500)
                     /locus_tag="VGB76_19305"
     CDS             complement(8806..10500)
                     /locus_tag="VGB76_19305"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEY0101081.1"
                     /translation="MRLLDWAVVVAYLVYIIWDGVRMTKHSGNAEGYFLANRSLPWWA
                     VGLSVMATQLSAITLVGTTGQAYNDGMRFIQFYYGLPLAMVILCITAVPFFYRAKVFT
                     AYEYLEKRFDLKTRTLTSFFFLISRGLGVGVIIAAPSVILSIVLGWSETATVFAMGLS
                     TTIYTMVGGVQAVTWTDVKQMIIIFFGLFVCLFIILAKFPPEVSLGDALHLAGTTGRL
                     TTVDTTFDLKQTYTLWSGLIGGLFLMLAYFGCDQSQVQRFLTAKSVGEGRTSLLMSAF
                     VKIPMQFLILLVGVLVFVFYQFQPPPMIFNRVEQAKVAEGSRAADYNALNAEYERAFA
                     ERQRAAVDYVKHEESDDAAARASAREAYLAANKGFNEARGHGLALVKESSGNKTFTDV
                     NYVFPTFVVQNMPAGVIGLIIAAIFAAAMSSISAELNALATATTIDFYRRHYKPQADD
                     RHYVKIGRIATGLWGIFACFVALYATTLGSLIEVVNRFGSFFYGSLLGVFVLAVAVPR
                     ARARGAFWGLLFGITSVWIASRFTEIAFLWFNVIGCLVVVLVGYLLSLTVRDPKSV"
     gene            complement(10525..10773)
                     /locus_tag="VGB76_19310"
     CDS             complement(10525..10773)
                     /locus_tag="VGB76_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101082.1"
                     /translation="MARTQAARALGNFGKRLFHPSAYNHDLKADHFFEDAMPESTSEK
                     ERELREEDARWHVIYVAVIVFAVLVITALYLFSRQFSS"
     gene            complement(10743..11249)
                     /locus_tag="VGB76_19315"
     CDS             complement(10743..11249)
                     /locus_tag="VGB76_19315"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY0101083.1"
                     /translation="MSATRSETVGRVGGEAAVASSWRSRLWRYLPLAAWTGFIFFAST
                     GAMSASNTSRIIAPLFRWLFPGINEAQLLLVHFTVRKTAHFTEYALLALLAARAFIPS
                     TRRLLRRSWFVAALALVALVALLDEYNQSFNAARTGTIWDSLIDISGGATALVALSLW
                     RARRRRVR"
     gene            complement(11432..12334)
                     /gene="atpG"
                     /locus_tag="VGB76_19320"
     CDS             complement(11432..12334)
                     /gene="atpG"
                     /locus_tag="VGB76_19320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY0101084.1"
                     /translation="MANLLDLRRRIKSVKNTQQITKAMKMLSAVNLRRAQERVTNARP
                     FARKMTEVLTELASHTDENYHHPLLDRRGDERYLLVLITADKGLAGAFNANLIKAAQA
                     FIREHEGKTVELMAVGRKGRDFFRRRDIQIVREYIGLTGKGRVSYEEAAEMAGDITRR
                     FTEDETLDKVFLLYNEFRSVLQQRVVVEQLLPVSRVEEQAGEGAEANSGEPQTFVEYV
                     YEQPPEEIFSRLLPRLIETQVFRALLESIASEQGARMTAMDAASKNAGDLIGTLTLNM
                     NRIRQAAITKEIIEVVSGAAAAQS"
     gene            complement(12420..14027)
                     /gene="atpA"
                     /locus_tag="VGB76_19325"
     CDS             complement(12420..14027)
                     /gene="atpA"
                     /locus_tag="VGB76_19325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY0101085.1"
                     /translation="MEPIRANEISDIIRQQIENFDTGVTVTEVGTVIKVGDGIAEIHG
                     LEKVMAGELLDFPHEVRGLALNLEEDKVGAVLFGEFQKIKEGDTVRRTGRIMDVPVGD
                     AVIGRVVNALGQPVDERGPILTDEFNPIERIAPGVVERQPVKEPLQTGLKAIDAMVPI
                     GRGQRELIIGDRQTGKTAVALDTILNQKGKDVICIYVAIGQKASTVAQVIKTLQTHDA
                     MSYSIVVNASASDPAAMQYLAPYAGAAIGEYFRDTGRHVLTIYDDLSKHAAAYREISL
                     LLRRPPGREAYPGDVFYLHSRLLERASKLSDLKGGGSLTSLPIIETQAGDISAYIPTN
                     VISITDGQIFLEADLFNAGIRPAINVGNSVSRVGGSAQIKAMKAVAGTLRLDLAQYRE
                     LAAFAQFGSDLDKATQSQLARGQRLTEILKQPQYQPIDVEKQVLIIWAASNGFLDDVP
                     VDQARRFEAELVRFMENAQAGLLQRIREKKALTDDIKADLKQALADFKDTWNVNDMAV
                     ATTGAQTPAAATSNATTPAAQTTAVGA"
     gene            complement(14116..14655)
                     /gene="atpH"
                     /locus_tag="VGB76_19330"
     CDS             complement(14116..14655)
                     /gene="atpH"
                     /locus_tag="VGB76_19330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEY0101086.1"
                     /translation="MQTMARRYAAALADVVIAQGEAREVQEELNSWAQMMEDNSTLLE
                     VFRNPTIPYEQKRNVLNTLIKRTRVRPTTANFLQVLLQNHRLSELNEINQKFAVELDA
                     RSGVVSAQVTTAREVPESTKETLRTQLGTLTGKKVRLQFAVDEDLIGGIVTRIGSTVY
                     DGSVRTQLQQIKQKMAGEQ"
     gene            complement(14658..15206)
                     /locus_tag="VGB76_19335"
     CDS             complement(14658..15206)
                     /locus_tag="VGB76_19335"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEY0101087.1"
                     /translation="MTYLFSHPFVLFALLAGDAPWYDYPGWEVWRFANLLLFIGVLFF
                     MLRRPIGASLVARRDAIRQELMRAQEERRAALAKLEEVEARLARLDAEVETVRAQAKR
                     EAEAERENITRSTAEETRRLREQARREIESAGKVARQDLRRYAAEQSVRLAEDLIRRD
                     IRADDDTRLMNEYIGDLGGIKG"
     gene            complement(15214..15699)
                     /locus_tag="VGB76_19340"
     CDS             complement(15214..15699)
                     /locus_tag="VGB76_19340"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101088.1"
                     /translation="MILLGLAGNSIQLVPDGTLFLHILIIVLMIYILNATLFRPVNKI
                     LAERERRTRGGTGEAQDILQRVANSMNRYERSLREARSEGYRLLEQERAKALLERQNQ
                     LSALRAEIESSTEEQKSLLRTQSEQARALLESDARKIAADISRQILNRPSPDFTATGV
                     R"
     gene            complement(16090..16986)
                     /locus_tag="VGB76_19345"
     CDS             complement(16090..16986)
                     /locus_tag="VGB76_19345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY0101089.1"
                     /translation="MTRKVLGRGLSALLSETPSATAANEELREVDIDLIEPNNVQPRT
                     RFDETQLEELAQSIKINGVVQPILVRKTAGGRYQIVAGERRWRAAQRAGLQRIPSVIR
                     DVPDDKMLELALIENIQRQELNAIEEAYAYKRLIETFNLTQETVAQRVGRDRTFVTNY
                     LRLLRLPEDIQRLVEESKLSMGHARALLGIDDADKQRQIARNIIERSLSVRDTERAVK
                     RVVAGEDSEPAPASASTERVSDANTRAAESKLRRRLGTQVHILPNQSGAGGKIEVQYY
                     NENDLQRVYELILGAQNISDTL"
     gene            complement(16913..17740)
                     /locus_tag="VGB76_19350"
     CDS             complement(16913..17740)
                     /locus_tag="VGB76_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0101090.1"
                     /translation="MAKIIAVANQKGGVGKTTTAVNLAAALAATEVRVLLVDTDPQAN
                     ATSGVGVQRGSFRRSLYHALILDEPLVNVILGTELPLLKVLPADRNLTGAEVELVDVE
                     RREYKLQGVLRNVQSEYDYIIIDCPPSLGLLTLNALTSADSLLVPIQCEYYALEGVTE
                     LFDTLARIRRSLNPGLIIEGLLLTMYDDRTNLSAAVAADLKSFYGRQVLETVIPRNVR
                     LAEAPSYGKPIILYDIRSRGAESYLQLAKEIISHDEKSSGAGLERAAVGDAIGDSGQ"
     gene            complement(17899..19773)
                     /gene="mnmG"
                     /locus_tag="VGB76_19355"
     CDS             complement(17899..19773)
                     /gene="mnmG"
                     /locus_tag="VGB76_19355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY0101091.1"
                     /translation="MSFDEQFDVIVIGAGHAGCEAASASARLGAQTALVTLNLDLIGQ
                     MSCNPAIGGIAKGHIVREIDALGGIMGRVIDRTGIQFRLLNRSRGPAVQAPRAQADRA
                     LYRTEMRQVLEETPNLHLRQGLVIELIVEDGQIHGVEMQDTRRLRAGAVVVATGTFLN
                     GLVHTGRRTYTAGRAGEPASIELAECLKRLGFPVGRLKTGTPPRLDGRTIDWHEFEPQ
                     LADERPVPFSFSTETIEQPQVTCFIGYTNERVHDSIRSNIHQSPLYSGQIKGVGPRYC
                     PSIEDKVVKFPEKTRHQLFLEPEGHHTSEVYLNGFSTSMPAEFQQELVHKIPGLEDAR
                     IIRPGYAIEYDFVDPRELTPYLQTARISGLFLAGQINGTTGYEEAACQGLLAGINAAL
                     YIQRRDGFRLTREESYIGVLMDDLITHGVDEPYRMFTSRAEHRLSLRYDTADARLTPY
                     GKKLGLVGDSLWDAFNARRERLAQLRNALETTRFKRTDATYRELAQLLGDLGDSISLA
                     QLSQRQNVTHELIRSLLPHPLQTETSYSDLETSLADLLYCGYVQSQKDSFARLFHNDA
                     LRLPSNFDFRSISGLSHEMVERLVRANPQTFGQARRIPGMTPAALSTLLVKLKLQQAA
                     "
     gene            complement(19779..>20449)
                     /locus_tag="VGB76_19360"
     CDS             complement(19779..>20449)
                     /locus_tag="VGB76_19360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY0101092.1"
                     /translation="SVFNSLLASDRAIVTEIPGTTRDSLSELLNIDGVPVLLTDTAGL
                     RESNDPVEYLGIERTRRAIADADLILFVLDGSQPLTDEDRAIFANIVEDKHLIALNKS
                     DLSNFDNLISAGIGDNSKCVAISAKTGEGLDALRAAILEPFKAGSAPETGFLITNARH
                     FDLLRRSSDALRSSKVLVEQRASEDLILVGLYESLRYLDEITGETTPEDVLSQIFATF
                     CIGK"
BASE COUNT         4241 a         6138 c         5901 g         4169 t
ORIGIN      
        1 tgcttctttc gccatgtatg aaggatgcca cccttaaaca agagttgcgg cgcaagcctc
       61 ttccgtgatg aggaaagata atcagggcgt gggtagtagg gcttacctgt ccaatccatg
      121 tgagagcgat ttgcagggtc ttgcatgtag tgctcaaaag gcgcacgcac attgccggag
      181 atgtaaacgg actgcacttc acacgatcca aaatcaatta cgcgtccttt gtcatcgtaa
      241 gaaactaata caacgtctat attaccggca gattggccgt atttatcttt aagtcgaacc
      301 tcaaccagtg atgtccatgt ggttgctggc gggaagaaga aagcggctgc gtcatcagcg
      361 atgagccaat cttcacgaaa acgaacggga caagtaattg ctacagcatc gctttcatgc
      421 acgctacaca cgccaagagg agcattggct ttgtccttcg tgcagttagg cactttgtta
      481 ttaaatgggc agagccgctt ttcttgatag cgggttgctt cgggcgattg gttgtctata
      541 ggaaagccga agacttccgc aagtggttgc ttaggcacac tatacctttc taaacaagat
      601 attcagataa taatgtcaaa ttaaaaggga gtctatcaaa ggtagattca gatcgtttta
      661 gctcaagaat caaaatttca cttcttacta cctttcctgc tcacccttga actctcagct
      721 ttagccttcg gcggtctgcc tactttgcgg tcttctacaa ggcttaagtc ttcttcttta
      781 attacatagt cgcgtcccac cttttcagcc ggtaaccgtt tttcgtttat taactgatgg
      841 acacgtggga tgctgatgcc caagcgttca gcggtctgct ttgtcgttaa aagtcccatg
      901 aaggtgatta tttatatgtc tgccgaaatc ctatatcaaa ttatagattt ctgttgacga
      961 acactagaag atgctatagt gtttcgccta cccacacgcg cactataaac tgtttgcaga
     1021 tacgtgtcac cgatgcaagt cagaaccgcc tctcgcccgt tggtaaacaa ctataaatag
     1081 gcgaggactt accaagacgg cgatctcgat aagtcctcta caccagtaac cactcgaaag
     1141 gaactactga tgcgaaaaga taatagcacg cgcacgccag ccgcccgccg acgagagccg
     1201 agttttgaag aacctatacg gcgttgggct aacctgccga ttacacaaga cagagcgagg
     1261 gagttggttg acttgatcac gaggcgcgag gatagagatg ctttggacgc cttgatggag
     1321 ttgatctgtg gggtgatgcc ttacgcgccg tctgacgaca agcatgattt ggatgagagg
     1381 gttctggccg gacacgctgc cctgtggcat gcgtttgcgg atacgacgcg ggcgcacaat
     1441 gacctcggcg cgtatgtcgt cgggttgaag tgcgccgagt gggaatagcg cggggcttcg
     1501 ttacgcgtga cgcgtggcgc gcgctacgac gcggcgcatg cgttcgctcg ctccgagcgc
     1561 ggcgatggcc gcttcggtcg caaactgcgg cgcaaacgcc acccgtcgca gccatgagca
     1621 gacgcgcaga cgcgcggcga accgctctcg atagcgtgcg cgatagtcct ccgccagtcg
     1681 ggcgaaaccc gcgcccgcgc gcaactgcgg cagcgcactt gtcacggcct cgcccgccag
     1741 ttcgccgctc tccaaagcca tcaacatgcc gctccccgtg aatgggtcta tgaacgaggc
     1801 cgcgtcgccc accatcaaca acccttccgc cgggaccacc tcgcgccgcc cgaaactctc
     1861 caagcccacc gccagccact ccgaacgcac gcgcgcgccg cgcagagtct ccgccgcacg
     1921 actgttcgac atcacgatct ctcgcatcac gcgcgccgca tcgctcgcac gcgcacgcac
     1981 gtcttgcgcc gccacgatga aacacaaatt actcaactca ttctccacgc aactcagccc
     2041 gccgtagccg cctcgataaa aatagatctc gcacgcgccc gccgcgcccc ctgcgttttc
     2101 gagatgcgtc ttgaacgcca cgagcggcat gcgtctcgac gccgcgccgc ccctcttcgc
     2161 ttcacgcgtc gcgccgccgg acaccttcac acgcggctcg acccggcgcg ccaacgcacg
     2221 cgcacgcccc gtcgcatcaa tcgtcacgcc tgcacgcaac tccgacatgc ccctgcgtga
     2281 ctcgacgcgc acgccgcaca ccgtgcgccg cgccgcgtct gcaaacaaca accccgtgac
     2341 ctgcgcttct tccagtacct ctacgcccac cgcacgcgcg cgctgcaaca aacgctcgtc
     2401 catctccgcg cggctcagac cgagcgcgcc cgccgcctct ttcccgaacc acgcgctcgg
     2461 cacacccgcg cgccgcccgc tctgcgcgaa aaaaagcgtc tcgccgacct gcgcgccgcc
     2521 cgctccgctc atctcctcca acacgccgag acgcgcgaaa tgcggcaggc actcaggcga
     2581 aataaactct ccgcacaact tcgcgcgcgg aaacttctcc cgctcgacga gcaacacgcg
     2641 cgcgccgcgc gtcgccaggt gcaccgccgc gctcgcgccc gctggaccac cgcccaccac
     2701 gatcacgtca taatcttttg tcaggctctc actcatgctc aactcacgct ccccttagct
     2761 cccgccgagc accagacgga aaggaaatcg ccgccgtacc tctacgttct ccagttgcgc
     2821 cgtctctgcc atccggcgca actctccggc gcgaaaactc cgccggatgg agagcgcgcc
     2881 gtcctcgcgc aagaggcgat tgtgtaagaa cagacgcccg accgtcgtgt agagaaaata
     2941 ggcgagggcg tgccggtgca aatctatgac gaacaggcgg cggcgcgcga cgcgcttgaa
     3001 ctcgcgcaag atttcgacga cggcttcgtc tcgaaagtgg tgcgtgaaga gtgagcagat
     3061 gacgtaatcg aaagaatcat ccgcgaacgg cagccgcaag gcgtcgccgc gcaccgctct
     3121 gatctcttcg aagtcgtgcg actcttccag aatcgcccgc gccgaacgct cgttcaactc
     3181 aagccccgtg agccgcgcac gccacccgcg ccgccgcgcc tcccgcgcca ccacacgcaa
     3241 caactcaccc gacccagccc ccacgtcgag cagcgaaaaa ctctgcacgc ccgactccac
     3301 gatctccggc acgagcgagg ttttcaacgc gcgcgcatct cccagccact tgttcacgcg
     3361 ccgcagttcg accagacatc cttcatactc ctccggcgtg tagtcgccct tgtccagatg
     3421 ctctaactca taactccgct cgctaaacat ataagatagt gacgcgtgac aagtgacgag
     3481 tgacgagttg aagacaaaga aaacttagga catcgcccgt cgctccttgc acgtcgagcg
     3541 tcgctcgcgg cgtatcgttc gttctaaact gttccccgtc tctcgtcact cgtcacctgt
     3601 cttcaactcg tcgcttgtct tcgactcgtc actcgtcact tgtcacttgt cacttgtcaa
     3661 accttctcta cgacgagcgc gctgttcttc gagccgaagc cgatgcagtt gcagagcgcg
     3721 acgcgcacgt ccgcttcgcg tgcgacgttg ggcacgtagt ccagatcgca cacggggtcg
     3781 ggttcgtcga ggttgatggt gggcgggatc atcttcgtgt tgatggcgca gagcgcggcg
     3841 gcgagtcccg ccgcgcctga cgcgccctgc gggtgaccga tctgcgattt cgtggccgac
     3901 atcgggatgg cgtgcgcgtc tgcgccgaag gcgagtttca gggcttgcgt ctcgatgcgg
     3961 tcgttgagtt gggtcgaggt gccgtgcagg ttgacgtaat caacttccga gggcgcgcgt
     4021 ccggcatccg tgagcgcgag gcgcatggcg cgtgccggtt cttcgcccgt ctcttccaga
     4081 cgcacgcgat gataagcgtc gcacgtcgca ccgtagccct tgatctcggc atagactttc
     4141 gcgccgcgcg cacgcgctcg ctcgtaatct tcgagcacgt aaatccacgc gccttcgccg
     4201 agcacgatgc cggagcggtc gcgcgagaac gggcgcgagg cgcgtgcggg cgcgtcgttc
     4261 cactccttcg tcaagacggt catcaagttg aagcccgcga ggatgcccgg cgcgatgggc
     4321 gcgtccacgc cgcccgcgag cataacctcc tgacgaccga gcacgatatg ttcggcggcg
     4381 taggcgatgg catccgtcga actcgtgcag cccgttgaga tgatgtgcga gaggccgcgc
     4441 aggccgaagg ccatggagag ttcggaagag agtccgccgt gtgtcgaagc ggggatcgta
     4501 tagacgctcg ctttcgtcgt cgcaccggag aagtagtatt tatactgctg ctctgtgaag
     4561 gcgagtccgc cgccgcccgt gccgagcacc acgcccgtcg cgcgccgctc cgccagactc
     4621 gcatccgaaa aatgaagccc cgcgtcttcc agagcctcgc gtgcggcggc gagcgcgagc
     4681 ggcacggtgc gcggcacatg cttgcggtct tttgcgccca cctgcgcttc ccagtcgaac
     4741 gcgcgcacct ccgctgcgat ctgaaccgcc gaattgctca cgtcgaaact ctcgatgcgc
     4801 cgcgcgccgc tctcgcccgc gacgagcgca cgccagaacg cttcgcgccc gatcccgatt
     4861 ggcgtcacac aacccatccc cgtgatgacg acgcggcgca gttgcttcag agttctcaac
     4921 atgtcggttt tttaaatctt cgcgaggaag tgtttcgata gtattgaggc aaacaccgca
     4981 gagcataaca tcacacgcaa gccccccgct aacatttaac ccggcctctc gggcgcagcc
     5041 aaaacttttg tttcacagtt gaaataaagt attgcacatg aaaagaagct atggtataag
     5101 ctgccgtcat caagacttgc ggtgcaaaat tttggttctg ttaaggagag aatttatgga
     5161 cggcagcacg aaacatattc cggagggcta ccatgccatc acgccttatt tacatgtccg
     5221 ggacgcagca cgcgccatag agttttacaa agacgtgttc ggggcgaccg aggttctacg
     5281 catggacgaa ccgggcggaa agatcgggca cgccgaaata cggatcggcg gctcccacgt
     5341 catgctggcc gacgagtttc cagagatgga cgtgcgcagc cccgaatcgt atggcggcac
     5401 gcccgtctcg ctcctgctct acgtcgaaga cgtggacgct ctggccgagc gtgccgtcgc
     5461 ggcgggcgcg aagctgctga gtccgctcga agataaattc tacggcgacc gcatgggcaa
     5521 actccaagac cccttcgggc acatctgggc aattgcgacg cacaaggaag acgtgtcgcc
     5581 cgaagagttg cagaaacgcg cgacggcggc gggcttgcac ggttgaaggc catcccccgg
     5641 ctgacggcaa aagcattccg gcgctaagcg cggggaggag cgcgaagtga gcgcagagga
     5701 gtgtcccatg cttcctaagt ctcaggtcaa aggcggcaac ttgagacttg agacttggaa
     5761 catgggactg gggagctact ctgcgctcat tccgcgctcc tctcccggcg tctgcgccgt
     5821 tgaagtcgtc gcccgtgcgg ttaatacccg tctaatctcg cgctcttctc gcggcggttc
     5881 gcgtggccgg tgccggagct ttcggcaggc tcagcaagtt ggcgaacagg cgatacgcgc
     5941 cggggactcc ggcggggagt tggcgaaacc aagcgtagga cgtgtagacg taatgaccgc
     6001 gaccgatgcg cgcatagacc tgaccgccgc gctgttcgcc ctcgcccgcg tcgtgtgatt
     6061 cgaggagcgg cgtgtaggcc gcgtcgaagg tggtgaagtt gtagaggctg cgctcctgca
     6121 cccagccctg ccagtcggcg tcgtcgatgc ggttcgggaa gttgaacgcg ggatgcgcgg
     6181 gctgcaagac acgcaccgga gcattctcgt caaccacgcg cgcaatcgtc gcagacccgc
     6241 ccgcgcgttg cggcgccact tccatcgcgg cgggcagcgg cggcagtttt ttcgcagcgt
     6301 agtccgggcg ttgatactgg acgatgagcg cgccgccgcg ccgtacaaac tccgataggc
     6361 gcgcgtgatg cgcgacgaaa tcggggcgcg tctgcgaagc gcgcacgccg acgacgatgg
     6421 tatcgaaacg agagagatcg cctgcggcga gttgccgctc gtcgagcaag gtcacggtta
     6481 atcccatccg gcggatcgct tccggcactt cgtcgccgct gcccatgatg tagccgacgc
     6541 gcacgctcgc cacgttcaga tcaaagacgc gcacaatcgt ctccgcgctc ctgtagtagt
     6601 agtgcgtctg gatgtgcggg taactgatcg cgtggcttgt ctggccgaag gttagcgatt
     6661 gcggcagatg cggctggcgc aaacgtcgtt caggcagaaa ggtggcggcg aaacttctca
     6721 cgtagtactc gcccgcgcgc gtgccgcgcg gaaccgtgaa ggtgtaggac tgcgttgcgc
     6781 gctcgccttc ggagttgaag ttgaagggcg tcgtctccgg cagccgccag ccgacgggca
     6841 gactgatgcc cgccccgccc gttgcaagca aactctccgt ctgtcccaaa gcaggctcga
     6901 cgttgttcac caactgtaga gtcaactgcc gcgtgcgttc gcgcgagcct tgcgggacga
     6961 tgacgagcgc ggggtcgagt tgcacgttga tctccggcac gacgttgacc tcgcgccaca
     7021 cttccccgcg cacgttgtcg agataacgat actggacggg cgcgctgatg gtttgaggcg
     7081 acccgcccac gtccatcact acctcagccg agacgggcgg cggcgcaaac ggctctgtct
     7141 tcggatcgtc tttatcccac acgtacatgt cgcgcctgcc tcgcggctca cgccccgaac
     7201 tcgccagcca gtagggctgc gtcagttccg catcgacggg cacgtgtacg cgaaagtatg
     7261 cgacggccag cgcggcctcg cgtgcgacaa aggccgtctc gcccttcgac ggctccgcga
     7321 gtcgttcgac ccgccacttc tccggcgcgc gcaggcgcaa ctcttcgacg cgccccacgc
     7381 gcggatcatc cgcaaagacg cgcacggcga cgacgaacga accgccgcgc gcaatcgtct
     7441 ccgcgtccgc gagcgcatcg agacgaacgc cgtgcgcgag tcgcaacgcc tcgctgaact
     7501 cgcgctcctt ttgcgcgagc agaaagtcgg cttcggcgcg cgcagcggga gaatctttaa
     7561 gttgtgcaag cgtccgtcgc gcctgtcgtg tgtggaggag tccggcggcg agcgcggcgt
     7621 ggatagtgcg cggcgcgagc gcgttgaaat gttcgagcgc gtgtttggcg gcgcggtcta
     7681 cctgttcaag ctctttcgag agcgcatcgt cgcggagtcc ggcggagcgg atgaggccgt
     7741 gcagcgtcgt gtcgagtccc tcgtagatgc tccgttcgtt ttgcgcgact tgaaacgggc
     7801 tgggcgcact tttgaccaga cgcacgcgcg acgtctgcgc gccgcgcaac tccagagcgg
     7861 cctcggcctg cgacttgtgc tggctgcgcc cctccatcgc cacttcataa aaactcctgc
     7921 cgagcagcgg atcgaaacgc cccgtgtcga tctctaccac gctcgcattc gttccctgcc
     7981 ctgtgctcgc accggcagcg gcgggtggct gtccgaagac gctcacgtaa agtttcttcg
     8041 cctgccacgg tcgcaagcct tctgcgagtt gttcggggaa cgcggcgggg tcggcggcgg
     8101 cggcgaaggc gagcggcgtt aagtagcctg cgaattgatg ttgaccatgg ccgtctgcgg
     8161 gcgtgccgtt ccagcgcgag atgatgacga gcgggcgaaa gcgtcgtatg acgcgcacca
     8221 tatctgccag cagttcgcgc tcgttccaac gctcagccgt ctcttcgcgc gtcttcgtga
     8281 aaccgaaatc gaaggcgcgt gtgaagaact gctcgccgcc gtcgagtttg cgcgcctgta
     8341 aaagttcttc ggtgcggatg acgccgagcg cgtcaaagag ttctgcgccg atgcgattct
     8401 gcccgccctc gccgcgattg agcgagaggt agccgacgcg tgcgccgtcg cctcgtgcga
     8461 gccgcgcgat cagtgcggag tcttcgtcgt ccgggtgcgc gcccgtgtgc aacacgctcg
     8521 ccgtcgtttg caaacgacgc aacagcaagc ccaacccgac cgcgccctcg ttgtaagtcg
     8581 tgcgcacctg cgcgcgtgtg acgaaggaca caggcgcgac gtaagagacg ccgcccgtcg
     8641 aaagtgtgaa gagacaggcg agcgcaatga gacgaataaa gagacgacgc ataaaaattt
     8701 tgccgcaccg gcaagagcta atctcaaaac gagacaatct caagaagaga tgatctcaag
     8761 acgacagaac aaccgctcgc ttcgcacgat cagggcagcg agcgtctata ccgatttcgg
     8821 atcgcgcacg gtcagactca agagatagcc gacgaggacg acgacgaggc agccaatcac
     8881 gttgaaccag aggaaagcga tctccgtaaa acggctcgca atccagacgg acgtgatgcc
     8941 gaagagcaag ccccagaacg cgccgcgcgc acgcgcacgc ggcacggcga cggcgagcac
     9001 gaagaccccc aagagcgaac cgtagaagaa cgagccgaag cgattgacga cttcgatgag
     9061 cgagccgagc gttgtggcgt agagcgcgac gaagcaggcg aagatgcccc acagccccgt
     9121 ggcgatgcgc ccgatcttca cataatggcg gtcgtctgcc tgcggcttgt agtggcgacg
     9181 gtagaagtca atcgtcgtcg cggtggcgag cgcgttcaac tccgcggaga tgctcgacat
     9241 cgccgcggcg aagatcgcgg cgatgatcag gccgatcact ccggcgggca tgttctgcac
     9301 gacgaacgtg ggaaagacgt agttgacatc ggtaaaggtc ttattgccgc tcgattcttt
     9361 gacgagcgcg aggccgtgcc cgcgcgcctc gttgaaacct ttgttcgcgg ccagataagc
     9421 ctcgcgcgcc gacgcacgcg ccgccgcatc gtcgctctcc tcatgcttca cataatcaac
     9481 cgccgcgcgt tgtcgttcgg caaacgctcg ctcgtattcc gcgttgagcg cgttgtagtc
     9541 cgccgcgcgc gagccttcgg ccaccttcgc ctgttcgacg cgattgaaga tcatcggcgg
     9601 cggttggaac tggtagaaga cgaagacgag cacgccgacg agcaggatca aaaactgcat
     9661 cgggattttg acgaacgcgc tcatcaacag agacgtgcgc ccttcgccaa ccgacttcgc
     9721 cgtcaaaaat cgctgcacct ggctttggtc gcaaccgaaa taggcgagca tcaaaaacag
     9781 cccgccgatg agtccagacc agagcgtgta ggtctgtttg agatcgaagg tcgtatcgac
     9841 ggtggtgagt cttcccgtcg tgcctgcgag atggagcgcg tcgccgagcg agacttcggg
     9901 cgggaacttc gcgaggatga tgaagagaca gacgaagagg ccgaagaaga tgatgatcat
     9961 ctgcttcacg tccgtccacg tcacggcctg cacgccgccg accatcgtat agatggtggt
    10021 cgaaagtccc atcgcgaaga cggtcgccgt ctcgctccaa ccgagcacga tggagaggat
    10081 cacggagggc gcggcgatga tgacgcccac gccaagcccg cgcgagatga gaaaaaagaa
    10141 gctcgtcagc gtgcgcgtct tcaagtcgaa acgcttttcc agatactcgt aggcggtgaa
    10201 gactttcgcg cggtagaaga acgggacggc ggtgatgcag agaatgacca tcgcgagcgg
    10261 gaggccgtag tagaactgta tgaagcgcat cccgtcgttg taagcctgcc ccgtcgtgcc
    10321 gacgagtgtg atggcggaaa gctgcgtcgc catgacggag aggccgaccg cccaccaggg
    10381 cagactgcga ttggcgagaa agtagccttc ggcgttcccg ctgtgtttcg tcatgcgcac
    10441 gccatcccag atgatataga cgagataagc gacgacgaca gcccaatcaa gtaggcgcat
    10501 gttcttttag tatgaagttc aaactcagga cgaaaactgg cgcgagaaga gatacaaggc
    10561 cgtgatgacc agcacggcga acacgatgac cgcgacatag atcacatgcc agcgcgcatc
    10621 ttcctcacgc aactcccgct ctttttccga cgtgctctct ggcatcgcat cctcaaaaaa
    10681 atgatctgcc ttaaggtcgt gattatacgc cgaaggatga aataaacgct tgccgaaatt
    10741 tcctaacgca cgcgccgcct gcgtgcgcgc cataaactga gtgcgacgag cgcggtcgcg
    10801 ccgcccgaga tgtcgatcag gctgtcccag atcgtgcccg tgcgcgcggc gttgaaggat
    10861 tgattgtatt cgtcgagcag cgcgacgagt gcgacgagcg cgagcgcggc gacgaaccaa
    10921 cttcgtcgca agagtctccg cgtcgaagga ataaaggcgc gtgcggcgag cagggcaagc
    10981 agggcgtatt ccgtaaagtg cgcggtcttg cggacggtga aatgaacgag gagcagttgc
    11041 gcttcgttga tgccggggaa gagccaacgg aagagcggcg caataatgcg cgacgtgttc
    11101 gaggccgaca tcgcgcccgt cgaagcgaag aagataaacc cggtccacgc ggcgagcgga
    11161 aggtaacgcc agagacggct gcgccaggaa gacgcgaccg ccgcctcgcc gcccacccgt
    11221 ccaactgtct cgctccgtgt ggctgacacc ggcacttagt ttccttcaac tacttaaacg
    11281 cacgcgacct gcgctcatca gtcgccgcga cgaatccgcc cgtttgataa agacgataaa
    11341 gacttagcgc agggcttaaa caaaaaggga agccacgcat gcgccgcttc ccttctcttt
    11401 caactttgaa atttacgctg ccgcctgcga cttaagactg tgccgctgcc gcgccggaga
    11461 cgacttcgat gatctctttc gtgatcgccg cttgacggat gcggttcata ttgagggtga
    11521 gcgtgccgat gaggtcgccc gcgtttttcg aggcagcgtc catcgccgtc atgcgcgcgc
    11581 cctgttccga agcgattgat tcgagaagcg cgcggaaaac ctgcgtctcg atgagacgcg
    11641 gcagcaggcg gctgaagatc tcctccggcg gctgctcgta aacatattcg acgaaagttt
    11701 gcggctcgcc ggagttggcc tccgcgcctt cgcccgcttg ttcttcgacg cgcgagacgg
    11761 gcaacagttg ttcgaccacg acgcgctgtt gcaacacgga gcgaaactcg ttgtagagca
    11821 gaaaaacttt gtccagtgtt tcgtcttcgg tgaaacgacg cgtgatgtcg cccgccattt
    11881 cagccgcttc ctcgtagctc acgcgcccct tgcccgtcag tccgatgtac tcccgcacga
    11941 tctgtatgtc gcggcgacga aagaagtcgc gccctttgcg cccgacggcc atcagttcga
    12001 ccgtcttgcc ttcgtgctcg cggatgaaag cctgcgcggc cttgatcagg ttggcgttga
    12061 acgcgcccgc caaacccttg tcggcggtga tgagtaccag aaggtaacgc tcgtcgccgc
    12121 gccggtcgag caacgggtga tgataatttt catcggtgtg cgaggcgagt tcggtgagca
    12181 cttccgtcat cttgcgtgcg aaggggcgcg cgttcgtgac gcgctcctgc gcgcgccgca
    12241 gattcaccgc cgaaagcatc ttcatcgctt tcgtgatctg ctgcgtgttc ttcaccgact
    12301 tgatgcggcg tcgaaggtct aaaaggtttg ccataagaga gaggttaaaa gttagaggtc
    12361 agaggttaga agagatcagt gttacctgac ctctggcctc cgatctctaa cctctgcgtt
    12421 taagctccga cggcggtggt ctgcgcggcg ggcgtggttg cgtttgaagt tgccgctgcg
    12481 ggcgtctgcg cgccggttgt cgccactgcc atgtcgttca cgttccaggt atctttgaaa
    12541 tccgcgagcg cctgtttgag gtcggctttg atgtcgtctg tgagcgcctt cttttcgcgg
    12601 atgcgttgga gcagtcccgc ttgcgcgttc tccatgaagc gcacgagttc ggcttcgaag
    12661 cgacgcgctt gatcaaccgg cacgtcgtcg aggaaaccgt tcgaggccgc ccagatgatg
    12721 agcacctgtt tctccacgtc aattggttga tactgcggct gtttcaaaat ctccgtcagg
    12781 cgttgaccgc gcgcaagctg cgactgcgtg gccttgtcca ggtctgaacc gaactgcgcg
    12841 aaggccgcga gttcgcgata ctgcgcgagg tctaaacgga gcgttcccgc cacggccttc
    12901 atcgctttga tctgcgccga accgccgacg cgcgagacgc tgttgccgac gttgatcgcc
    12961 ggacgaatgc ccgcgttgaa gagatcggct tcgaggaaaa tttgaccgtc cgtgatcgag
    13021 atcacgttcg tcggaatgta ggccgagatg tcgcccgcct gcgtctcgat gatcgggagc
    13081 gaagtgagcg aaccgccgcc cttcaagtca gagagtttcg aggcgcgttc gagcaggcgc
    13141 gagtgcaggt agaacacgtc gcccggataa gcctcgcgtc ccggcggacg acgaagcagc
    13201 agagagattt cgcgataagc cgccgcgtgc ttcgagaggt cgtcgtagat cgtgagcaca
    13261 tggcgtccgg tgtcgcggaa gtattcgccg atggccgcgc ccgcgtaagg cgcaagatac
    13321 tgcatcgccg ccgggtcgga cgccgacgcg ttgacgacaa tcgagtagct catcgcatcg
    13381 tgcgtctgca aagtcttgat cacctgcgcc accgtcgaag ccttctggcc gatggcgaca
    13441 tagatgcaga tcacgtcctt gcccttttga ttgaggatcg tgtcgagcgc aacggcggtc
    13501 ttgcccgttt ggcggtcgcc gatgatcaac tcgcgctggc cgcgaccgat gggcaccatc
    13561 gcatcaatcg ccttcaagcc ggtctgcaaa ggctccttca caggctggcg ttcgaccacg
    13621 cccggcgcga tgcgttcaat cggattgaac tcatccgtga gaatcggacc acgttcgtca
    13681 accggctgcc cgagcgcgtt gacgacgcga ccgatgacgg cgtcgccgac gggcacgtcc
    13741 atgatgcgcc cggtacgacg caccgtgtcg ccttccttga tcttttgaaa ttcgccgaag
    13801 agcaccgcgc cgactttgtc ctcttccaga ttcaaggcca gcccgcgcac ttcatgcggg
    13861 aagtcgagca gttcaccggc catcactttt tccagcccgt gaatctccgc gatgccgtcg
    13921 cccactttga tcaccgtgcc gacttccgtg acggtgacgc ccgtgtcgaa gttttcgatc
    13981 tgttggcgaa taatgtcgct tatctcgtta gctctgatcg gttccatata gtttgatcca
    14041 gttcgtcttg ttaactttct tcacttgccc gttttgcgat ctccgttttc ggctcggaaa
    14101 tcgcaactca aaagattact gctcgcccgc catcttctgt ttgatctgct gcaactgcgt
    14161 gcgcaccgag ccgtcataga cggtggagcc gatgcgcgtg acgatgccgc cgattaaatc
    14221 ttcatccacc gcgaattgaa gccgcacctt cttgcccgtc aaggttccga gttgcgtccg
    14281 cagagtctcc ttcgtcgact cgggaacttc tcgcgccgtc gtcacctgcg cagagacgac
    14341 gccggaacgc gcatcgagtt cgaccgcaaa cttctgattg atctcattca actcggaaag
    14401 ccggtgattt tgcagaagga cttggaggaa gttggccgtc gtcgggcgca cgcgcgttcg
    14461 cttgatgagg gtgttgagaa cgttgcgttt ttgctcgtag ggaatggtcg ggttgcgaaa
    14521 gacttcgagc agcgtggagt tgtcctccat catctgcgcc caggaattta gttcttcttg
    14581 aacctcacgc gcttccccct gcgcgatcac gacatccgcg agtgctgccg cgtaacgccg
    14641 cgccatcgtt tgcacgctca acccttgatc cctcccaagt ctccgatgta ttcgttcatc
    14701 aaacgcgtat cgtcgtccgc acgaatatcg cggcgaatca aatcttctgc caggcgcacg
    14761 ctctgttcgg cggcgtaacg acgtaaatcc tggcgcgcca ctttgcccgc gctctcgatc
    14821 tctctgcgcg cttgctcacg caggcgacgc gtctcctccg ccgtcgaacg agtgatgttt
    14881 tcgcgttccg cttcggcttc ccttttggct tgcgcgcgca cggtttcgac ttccgcatcg
    14941 agccgcgcca aacgcgcttc gacttcttca agtttagcca gcgcggcgcg acgctcttcc
    15001 tgcgcacgca tcaactcttg cctgatcgca tcccgtctcg ccacgagaga ggctccaatc
    15061 ggacggcgca acataaaaaa gagcacgccg atgaagagca acagattggc aaacctccag
    15121 acttcccacc ccggatagtc ataccagggc gcgtcgcccg ccagcagcgc gaaaagcaca
    15181 aaaggatggc tgaagagata agtcataaga gtttcagcgc acgccggtcg cggtgaaatc
    15241 aggcgaaggg cgattcagaa tttgacggct gatgtctgcc gcgatcttac gcgcgtcgga
    15301 ctcaagcaaa gcgcgcgcct gctcagattg agttcgaagc agactcttct gctcttcggt
    15361 cgaagactcg atttccgcgc gcagggcgct tagctgattc tgccgttcga gtaaggcttt
    15421 cgcgcgttcc tgctcaagca gacggtatcc ttcactgcgc gcctctcgca acgaacgttc
    15481 gtaccgattc atactattcg ctacgcgctg caaaatatct tgcgcctcac ccgtaccgcc
    15541 gcgcgtgcgc cgttcgcgtt ccgctaaaat tttattgacc gggcgaaaga gcgtggcgtt
    15601 caaaatatag atcatcaaaa cgatgatcaa tatatggaga aacaacgtgc cgtccggcac
    15661 aagctgaatc gaatttccgg caagacccag aagaatcatg atgcccccac gcagttctca
    15721 cgaaccggtg caacactcag aggtaagata gtaaaactgt atgtatcaaa aggttcgaag
    15781 tttagcatgc gaatttaagc gtggtcaaga caacggcaag cggatcgatt cgacgcgccg
    15841 tactcctccc gcgcctccca actaccctca cctgagaagc gtattcgcaa aaatacagtt
    15901 caatacttca atgacccgat agtcaagaaa ttttaaaggc gtgatgaagt tttgctcaga
    15961 cgagtgagat tgattgtttc tcttggcaga acaaccttat aaaaaccagc gcaactaata
    16021 ctgagttttt ctacgaccac ccagcagctt taacttttaa aactaactta accctaaaca
    16081 atcaaacact tacaatgtgt ccgaaatatt ttgagcacct agtatgagtt cataaacgcg
    16141 ttgaaggtcg ttctcgttgt aatattgcac ttcgattttg cctccggctc cggattgatt
    16201 cggcaaaatg tgaacctggg ttcccagcct gcgacgtaac tttgattccg cagcacgtgt
    16261 attcgcgtcg ctgacccttt ccgtagaagc ggacgcgggt gcgggttcgg agtcttcacc
    16321 cgcaaccacg cgtttcacgg cgcgctctgt atctctaacg gataagcttc gctcaataat
    16381 gttgcgcgcg atctgcctct gcttatcggc atcgtcaatt ccgagcaaag ctctggcatg
    16441 acccatcgac aacttacttt cttcgaccag acgttgtata tcttccggta aacgtaataa
    16501 cctcagatag ttagtgacaa aagtgcggtc gcgcccgacg cgttgagcca cggtttcctg
    16561 cgtcaaatta aacgtctcta tcaggcgttt gtaagcgtaa gcctcttcga tagcatttaa
    16621 ctcttgtctc tggatattct cgattagagc cagttccaac attttatcgt cggggacatc
    16681 gcgtatgacg gaaggtatac gttggagtcc tgcgcgctgg gcggctcgcc aacgcctctc
    16741 gcccgcgact atctggtagc ggcctccggc tgttttccgc accagtatcg gctgcaccac
    16801 accgtttatc ttgatagact gcgccaactc ttccaactgt gtttcatcaa aacgtgttcg
    16861 cggctggaca ttattcggct ctatcaggtc aatatcgacc tctctcaact cttcattggc
    16921 cgctgtcgcc gatggcgtct ccgacagcag cgcgctcaag ccccgcccca gaacttttct
    16981 cgtcatgact gattatctcc ttcgccaact gaagatagct ctccgccccg cgcgaacgta
    17041 tatcatacaa aataataggc ttaccgtaac taggggcttc cgccaaacgc acatttcgag
    17101 ggatgactgt ttccagcacc tgtcgcccgt aaaaactctt caagtctgcc gcgaccgccg
    17161 ccgaaaggtt cgtccggtcg tcgtacatgg tcaagagcaa gccctcgatg atcaagccgg
    17221 gatttagaga gcgtcttatt cgagccagcg tatcgaacaa ctctgtgacg ccttcaagcg
    17281 cgtaatactc gcactggatg ggaaccagca gcgaatcggc gctcgtcaga gcgttgaggg
    17341 tcagcaagcc gagcgatggc gggcagtcaa taataatata atcgtattca ctctgaacgt
    17401 tccgcaggac gccttgtaac ttgtattctc tacgctctac atcaaccaac tccacttccg
    17461 cccctgtcag gtttctgtct gccggtagaa cttttaaaag aggcaactcc gttccgagga
    17521 tcacgtttac cagcggctcg tcgagaataa gtgcgtggta gaggctgcgc cggaaactgc
    17581 cgcgttggac gccgacgccg gaagtcgcgt tggcctgtgg gtctgtatct acgagaagga
    17641 cgcggacttc ggtagcggcg agcgcggcgg ctaaatttac agccgtggta gttttcccga
    17701 cgccgccttt ttggttcgcg accgcgatga ttttagccat tatttccact cggtgcagga
    17761 acgacaagaa atgtggctta aactaacaca aagttttccc acgtgctact tttttgtcct
    17821 taacccgcac gaaattgccg tcctcagatg tttcacgtgt cacattgagc tggtaatgtg
    17881 acacgtgaaa catacgggtc aggccgcctg ttgcagtttt agcttgacta aaagcgtgga
    17941 taaagcggcg ggcgtcattc cggggatgcg tcgcgcctgt ccgaaagttt gaggattggc
    18001 gcggacgaga cgctcgacca tctcgtgcga gaggccgctg atgctgcgaa aatcgaaatt
    18061 ggagggcaga cgcagagcat cattgtggaa gagacgcgca aaactatctt tttgcgactg
    18121 gacgtagccg cagtatagaa ggtctgcgag ggatgtttcg aggtcggaat aggaagtctc
    18181 ggtttgtagc gggtggggaa gcaggctacg gatcaattca tgtgtaacgt tctgacgttg
    18241 cgacaattga gccagcgaaa ttgagtcgcc tagatcgccc agaagttgag cgagttctcg
    18301 atatgtggca tccgtgcgct tgaaacgcgt cgtttcgagg gcgttgcgta attgagcgag
    18361 acgttcacgg cgggcgttga aagcgtccca gagagagtcg ccgacgaggc cgagtttctt
    18421 gccatagggt gtcaggcggg cgtctgcggt gtcgtaacgg agtgacagac gatgctccgc
    18481 acgcgaagtg aacatacgat agggttcgtc caccccatga gtgattagat cgtccatcag
    18541 aacgccgatg taagactcct ccctcgtgag acggaaacca tcgcgtctct gaatatagag
    18601 cgcggcgttg atgcctgcga gcaagccctg acaggcagcc tcctcgtagc cggtcgtgcc
    18661 attaatctgc ccggccaaaa acagcccgct gattcgcgcg gtctggaggt agggggtaag
    18721 ttcgcgcggg tctacaaagt catactcaat ggcgtagccg ggacggatga tgcgtgcatc
    18781 ttcgaggccg ggaattttat gcacgagttc ctgttgaaac tcggcgggca tcgaggtcga
    18841 aaagccgttc agatagactt ccgaagtgtg gtggccttcg ggttcgagaa aaagctgatg
    18901 gcgggtcttc tccggaaact taacgacctt atcttcaatc gaaggacaat aacgggggcc
    18961 gacgcctttt atttggccgg aatagagcgg cgattgatga atattcgacc ggatagaatc
    19021 gtggacacgc tcgttagtgt atccgatgaa gcaggtaact tgcggctgct caatagtttc
    19081 tgtcgagaag gagaagggaa cggggcgttc gtccgcaagt tgcggctcaa actcgtgcca
    19141 gtcgattgtc cgcccgtcga gacgcggggg tgtgcctgtc ttcaagcgtc ccacggggaa
    19201 accgagacgt ttgagacatt ctgccagttc gattgaggcc ggttcgcctg cgcgccccgc
    19261 ggtgtaggtg cgccgacccg tatgaactaa accgttaaga aaagtccctg tggcgacgac
    19321 aactgcaccg gcgcgtagac gccgcgtgtc ctgcatttcg acaccgtgaa tttgcccgtc
    19381 ctccacgata agttcgatca caagcccctg acgcaggtgc agattcggcg tctcctcaag
    19441 aacctgacgc atctctgtgc gatacagagc gcggtcagcc tgagcgcgtg gagcctggac
    19501 ggcggggcca cgtgaacgat tcaggagtcg aaactgaatt ccggtgcggt cgatgacgcg
    19561 tcccatgata ccgccgagcg cgtctatctc gcggacgata tggcctttgg caatcccgcc
    19621 aatggccgga ttacatgaca tctgaccgat gaggtcgaga ttgagcgtga ccagagccgt
    19681 ctgcgcgccg aggcgtgcgg aagcggaagc ggcctcgcag ccggcgtggc ctgcgccgat
    19741 gacgatcacg tcgaactgtt catcaaaact cataactact actttcctat gcagaaggtg
    19801 gcaaatatct gtgagagaac gtcttccggc gttgtttcac ctgtaatctc atcgagatag
    19861 cgaagcgatt cgtaaagacc gacgaggatc aagtcttcgc tggctcgctg ctcgacgagg
    19921 acttttgaag aacgcagcgc gtcggacgaa cgacgcaata agtcgaaatg gcgcgcgttg
    19981 gtgatcaaaa aacctgtctc gggcgcgctt ccggccttga acggttcgag gatggcagcg
    20041 cgtagagcgt cgagtccttc cccggtcttc gcagagatag cgacgcactt agaattatca
    20101 ccaatcccgg cagagatcaa attatcgaaa ttgctcaagt cgctcttatt caacgcgatc
    20161 agatgcttat cttccactat gttggcaaat atcgcccggt cttcgtccgt caggggttgg
    20221 gagccgtcga gcacgaagag aatcaggtcg gcgtcggcga tggcgcgtcg tgtacgttcg
    20281 atgcccaaat attccacagg gtcgttcgac tcacgcaggc cagccgtgtc cgtgagtaag
    20341 accggcacac cgtcaatatt taacaactcg ctcaaactgt cccgggtcgt gcccggaatt
    20401 tcggtcacga tagctctgtc tgaggcaagt aaactattga aaacgctag
//
LOCUS       DASWNS010000559         5893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_125371, whole genome shotgun sequence.
ACCESSION   DASWNS010000559 DASWNS010000000
VERSION     DASWNS010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125371"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..360)
                     /locus_tag="VGB76_19365"
     CDS             complement(112..360)
                     /locus_tag="VGB76_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101093.1"
                     /translation="MSEKNGPAPKALGVAGAAKYLGVSHYKITRFIKLGLLQAHKDLN
                     DERQKLIKISDLDRLKRQLESPVGDADVEGRELNSAAA"
     gene            983..2233
                     /locus_tag="VGB76_19370"
     CDS             983..2233
                     /locus_tag="VGB76_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101094.1"
                     /translation="MDGEPVFVCQISEETIYRQGTAVKPFICRGLTMSARALEAPRFV
                     RRTALGPNEYRKNFRQIDGDVISAVTSREADGVTPLMSKIYLRLIHAPARYWEREGVL
                     RFEAGLSGDGQVKAWDALCEVAGVASATASKALRWMHEQGIIGYFAGKNGVGIRIFLN
                     RATSSIGSRVAGKKILEFPPTSSREARASSGEAAFSDSYADIEVSDTDRNPGAPKDGA
                     ETKPVGKTSPSPTRPSTSVSCTTHKREEERQPTGRPVTTAVPVEEIVGRLRRELEPRL
                     REAAAGAAAQSAAREMERTREWFETKALPKAVRVAQRETYDLLRKHGGVDGRRGRARA
                     GLEVGRAAEDYTTAAAARPMTPEEIRETAEVCVALLETQGKSIEVTLSEISSEGGGWL
                     LPEDAPRVRAAAAELALYWEEREA"
     gene            complement(2478..4550)
                     /locus_tag="VGB76_19375"
     CDS             complement(2478..4550)
                     /locus_tag="VGB76_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101095.1"
                     /translation="MNSYCFQPIFHLIESTPEFRALLYADVRKIGKYLIGYARREGIA
                     IEVLTLLDLCDERAKILPSFVAFINERPRPKGKSEKEHVKYGKQILSRIKKIIKVLAA
                     GLPNIQEVTGLPDDLLRSVPEFLRPVLIYLPRGNRRSALSPARLASPLTDRARLSLKA
                     ILAVTKSKGITTLYELFVTYWADLRTACYAGSPPADAYKVYSTLIHIRSNFGLHKKKS
                     TRAFSLDDAPPRLREQIVKYMRLAPSGASGEPELRRLGLSKGIALERHEQSTIHAQVY
                     CFLALLGRIPLREDMEIIDFIRMESRTVVEDGEEVPQLYNRIIDPFRQEERDKETPNK
                     PQGFDSVLFRSAYLAIKSIAAYNGIFRHHKSFSEVYKTNLDHKGREDRKDEKKEAFPM
                     SWLDYNLKLLRPQFDAIIADGSFKTDIEALEKCMCYVYLMMLRYTGVRQQGLRVCKIG
                     WNVMFNKNGSITFHWRVDEIKNDKDFTCTIKPGHTSTFALLVKTLRRYRQHIYPFLVD
                     TAHKAGVDLEGQLFIEVLADGSVRRFGNDHDGSRKFADTFKNLTSRFLDFTDIKPAMR
                     RKFNAHHIRGIAGDWMHHDLGVGTEGTAKFLANNRLTTERLYVDKKRIDSTQVLDEAD
                     KRAALKKAGHSTFSADIIDTATPGSAHEQQIAHLTAQNEVLQSKVDIVTTLLEDMASR
                     ERNNSTSD"
     gene            complement(4886..>5893)
                     /locus_tag="VGB76_19380"
     CDS             complement(4886..>5893)
                     /locus_tag="VGB76_19380"
                     /inference="COORDINATES: protein motif:HMM:NF025240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="strawberry notch C-terminal domain-containing
                     protein"
                     /protein_id="HEY0101096.1"
                     /translation="EVIHRLTDAQRRMYDAMAAAWQIIFQNIEQALEITNGDNRARAN
                     AMQKFWGDHQRCCRQVISAFKVPALIEEVEREIEAGHACVVSLIGTGESKTKEQVTKA
                     TAAGGHIEDLDFSPREMICGMVDRGFPTTQYVERTDPSTCQTTYVPLLDGEGKAVESK
                     EAVRMKRMLLDQLSGLELPEHPLDQIINHFGVLKVAELTGRKRRLIRTREGKLEYVKR
                     NPDGVAMQNTNLHEMALFQEGKKDIAIISDAASTGISLHADKRAGNRKRRVHITLELN
                     WSADKQLQFSALFGKKPLIPMLSTANRRRCNRGTEWTWFHSNTFTLGNFHCGSYGLTS
                     C"
BASE COUNT         1233 a         1599 c         1675 g         1386 t
ORIGIN      
        1 gttgtgctct tacccacccc gcctttctgg ttcgcgattg tgatcgtgat catcattcga
       61 acaccgctat tggagtcttt cccgacccgc gactccggcc ggctctaaac ctcaggcggc
      121 cgcactgttc agttcccttc cctcgacgtc ggcgtcgccg accggagatt cgagctgcct
      181 tttcaggcga tctaagtcgg agattttaat cagcttctgg cgttcgtcgt tcaaatcctt
      241 gtgagcctgg aggagtccga gtttgatgaa cctggtgatt ttgtagtgtg aaacccccag
      301 gtatttcgcc gcgccggcga cccctaaagc tttcggagct ggaccattct tctcagacat
      361 aaaccctgcc ttcgtgccct gatgattcga gattaattca gacgaaccga gatattccga
      421 gacgattttt gaattatcgt cactattcac gagatgtcaa gaatattttt cctctatccc
      481 cttgaagaat ttacctttct cccgtcggcg cgctcgccgg agccctgcca ataaaatcac
      541 acccccctgc taaaataatg acagggcatg ctaatatttt tacatcccct gctattttat
      601 tcgcattccc tgttctatta atcacagtcc gtgctgcttt aataacatgg gctgtgattt
      661 atttgtcagg ccatgcgttt ttggtcgcat cccctgtcga actttcatca gggcatgcga
      721 ggcgaatagc acaccctgtc aatcatctcg cagggggtga tgataattcc tcagactatg
      781 cgaaaagttc gacaggggat gacgcgtgcg tcacatggcc tgcgagaaaa acggcagagt
      841 atgcgtaaaa aatagcagtc ttctcaaagg cggggaagtg gtgtaagagt gcggggtatg
      901 cttgccgggc ggcaggcgaa aagcaaatga cctgaagctc tgagtttggc gaccgcggct
      961 tcaggtcatt cgggtaactc gcttggacgg cgagccggta tttgtgtgtc aaatctcgga
     1021 agagactata taccggcagg ggacggccgt caagcctttt atttgcaggg gtttgaccat
     1081 gagcgcacgc gcacttgaag cacccagatt cgtccgccgg acggccctcg gcccgaacga
     1141 ataccgcaag aacttccgac agatcgacgg cgacgtcatt tcggccgtca cctcgcggga
     1201 ggccgacggc gtcactccac tgatgagcaa gatctacctg cggctcatcc acgcgcccgc
     1261 gagatattgg gagcgggaag gggttcttcg ctttgaggcc gggctgagcg gcgacggcca
     1321 ggtcaaggcg tgggatgcgc tctgcgaggt ggccggcgtc gcgagcgcca cggccagcaa
     1381 agcgttgcgc tggatgcacg agcaggggat tatcggctac ttcgccggca agaacggggt
     1441 gggcattcgc atcttcctca accgcgccac gtcttcaatc ggcagccgtg tggcgggcaa
     1501 aaaaattttg gagtttcccc ctacttcatc ccgagaggct cgtgcttcat cgggtgaagc
     1561 agcctttagt gacagctacg ctgatataga agtttcagac acagatagaa atccgggcgc
     1621 gccgaaagac ggcgcggaga caaagccggt tggtaaaacg tctccatccc caacccggcc
     1681 atcaacctcc gtttcttgca cgacccacaa acgcgaagag gagagacagc ccaccgggcg
     1741 accggttacc accgccgtcc cggtcgaaga gattgttgga cgcttgagga gagaattgga
     1801 gccccgcttg cgcgaggccg cagcgggcgc cgccgcacag tccgccgcgc gtgagatgga
     1861 gcggacgcgg gagtggttcg agacgaaggc gctgccgaaa gccgtgcgcg tggcgcagcg
     1921 cgagacctac gacctgctgc gcaagcacgg gggagtcgac ggccgacgcg gacgggcacg
     1981 cgccggactc gaggtcgggc gcgccgcgga agattacacg acagcggcgg ctgctcggcc
     2041 gatgacgcct gaggagattc gagagacggc cgaggtgtgc gtcgcgctcc ttgagacgca
     2101 gggtaagtca atcgaggtga ctctctccga gatcagctcg gaaggaggcg gatggctcct
     2161 gccggaggat gcgccgcggg ttcgtgcagc ggcagcggag ttagcacttt actgggagga
     2221 aagggaagca tgaccgtcgc tttcaaatcg atgtaccccc cctttttatg gacagtcagg
     2281 tggaatgact ctatttcacc catctgtgcc acatgggcta atctgacgac gaaaggcaac
     2341 actcaatcag cgacattgca ctcagccatg cagcctatct gtttgtaaag ttctgtctaa
     2401 tatgggtcgg ataattggcg cggctatatt tatcctaccc tattagaccg aaagagggtc
     2461 attacttaaa catagattca atcactggtg ctgttgttcc tctccctaga agccatgtct
     2521 tccagcagag tcgttacaat atcaacttta gattgaagca cctcattctg ggcggtgaga
     2581 tgagcgatct gctgttcatg cgccgatccc ggtgtggccg tatcgatgat gtcagcagag
     2641 aaagtgctat gacctgcctt ttttaaggcc gcacgtttat ccgcctcatc gaggacttgg
     2701 gtactgtcaa tccgtttttt atccacgtac aaccgctcag tggtcaggcg gttattagcc
     2761 agaaacttag ctgtgccttc ggttcctacg cccaagtcat ggtgcatcca gtcgccggcg
     2821 atcccacgga tgtgatgcgc gttgaactta cggcgcatcg ctggcttgat atccgtgaaa
     2881 tccaggaacc gagaggtcag atttttgaat gtgtcagcaa attttcttga tccgtcatga
     2941 tcattcccga agcgacggac cgacccgtcg gccaacactt ctatgaataa ctgaccttca
     3001 agatcgacgc cggccttgtg cgccgtgtca accagaaagg ggtagatgtg ttggcggtaa
     3061 cgccgcagcg tcttgactaa aagggcgaac gtcgaggtgt gtccgggttt gatggtgcag
     3121 gtgaaatcct tgtcgttctt tatttcgtcg acccgccagt ggaacgtgat agatccattc
     3181 ttattaaaca taacattcca gccgatctta cagacgcgca gcccctgttg cctcacaccc
     3241 gtgtaacgca gcatcatgag gtagacgtag cacatgcatt tttcaagtgc ttcgatatca
     3301 gtcttgaagg acccatccgc aataatcgca tcgaactgtg ggcgtagcag cttgaggttg
     3361 taatccagcc atgacatggg gaaggcttct ttcttttcat ctttcctatc ctcgcgccct
     3421 ttatggtcga ggttggtctt atagacttca ctgaaacttt tgtggtgacg aaagatgccg
     3481 ttgtatgccg cgattgactt gatagccaga taagcgcttc ggaagaggac gctgtcaaag
     3541 ccctgcggtt tgttaggtgt ctccttgtcc ctctcctcct gccggaaggg atctatgatc
     3601 cggttgtata attgagggac ttcttcaccg tcttccacga cggttctact ctccatgcgg
     3661 atgaaatcaa ttatctccat gtcctccctc agtggtatgc gccccagtag tgctaagaaa
     3721 cagtagactt gtgcgtggat agtgctctgc tcatgccgct ccagggcgat acccttgctc
     3781 aggccaagtc gacgcaattc tggctcaccg gacgcgcccg atggcgccag tctcatgtac
     3841 ttcactatct gttctctgag gcggggcggg gcgtcgtcta ggctgaacgc gcgagtcgac
     3901 ttcttcttat gaagaccaaa gttagagcgg atgtggatga gggtgctgta aaccttataa
     3961 gcgtccgccg gtgggctgcc ggcatagcag gcggtgcgca gatccgccca ataggtcaca
     4021 aaaagttcgt agagcgtggt gatcccttta gacttcgtca cggctaagat ggctttaaga
     4081 gacagcctcg cgcggtcggt aagaggagag gcgaggcggg ccggtgataa cgcgctacgc
     4141 cggttccccc tggggagata gataaggact ggcctcagga attccgggac ggaccgcagc
     4201 aagtcatcgg gaagccctgt aacctcttgg atattaggaa gtcccgctgc taatactttg
     4261 atgatctttt taatgcgtga cagaatttgc ttcccgtact tgacgtgctc cttttcactc
     4321 tttcctttcg gccgggggcg ctcgttgata aatgcaacga aggacggcag tatctttgcc
     4381 ctctcatcgc acaggtcgag aagagtcaaa acctcgatgg ctatcccctc gcgacgtgcg
     4441 tatccgatta aatacttccc gattttgcgt acgtcagcgt agaggagcgc tctgaattcc
     4501 ggggtcgatt cgataaggtg aaagatgggc tggaagcaat aactgttcat gatgtcctcg
     4561 ggagtgttgc gaggacacgc caatcgccgt ctagtcactc cgcctacgtt taaacagaca
     4621 actaccttcc acaccaagca catttccgct gacacacgtg cagatacgtg agtgcgtacc
     4681 ggtgcgattg tcgctttgtc tttggtagtt cttagacgta ggtggcgcta acgggtcaac
     4741 acttaatatc cataatggta actctaagcg ctgactcgcg tgacatcaat cggactctta
     4801 ctgtaatgcg gtggtaagag tcagggcatc gattggtgtg cgctacgcga atgttaagta
     4861 aagcgaaagg gcctggggta ggtgactagc acgaggtcag tccgtacgac ccgcagtgga
     4921 aattaccaag ggtaaaagtg ttggaatgaa accacgtcca ttcagtaccc ctgttacatc
     4981 tccgccgatt cgctgttgat aacattggga ttagcggctt ttttccaaaa agggcggaaa
     5041 actgcaattg tttatcagca ctccaattta gctctaaagt gatgtgaaca cggcgtttac
     5101 ggttcccggc gcgtttgtct gcgtggagtg aaattccggt cgaagcggcg tcggaaatga
     5161 tggcgatatc cttcttcccc tcctggaaga gcgccatctc gtggaggttc gtgttctgca
     5221 tcgcaacgcc atccgggttg cgcttgacgt actctaactt gccctccctg gtgcgaatga
     5281 gccggcgctt gcggccggtc aactccgcga ccttcagcac cccgaaatgg ttgataatct
     5341 ggtccagggg gtgctcgggc agttcgagcc cggagagctg gtcgaggagc atccgcttca
     5401 tgcggacggc ctctttgctc tcgaccgcct taccttcgcc gtcgagcagc gggacgtagg
     5461 tcgtctggca ggtgctcggg tcggtcctct cgacgtactg cgtggtcggg aagccgcggt
     5521 cgaccatccc gcagatcatc tcgcgcgggc tgaagtcgag gtcttcgatg tggccgcccg
     5581 cggccgtcgc tttagtcacc tgctccttcg tcttcgactc gcccgtgccg atgagcgaga
     5641 cgacgcaggc gtggccggcc tctatctcgc gctcgacttc ctcgatgagg gccggcacct
     5701 tgaaggctga gatgacctgg cggcagcagc gctggtggtc gccccagaac ttctgcatgg
     5761 cgttggcgcg cgcgcggttg tcgccgttcg tgatctccag cgcctgctcg atgttctgga
     5821 agatgatctg ccacgccgcg gccatcgcgt cgtacatgcg gcgctgcgcg tcggtgagcc
     5881 tgtggatgac ctc
//
LOCUS       DASWNS010000560         5019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_489998, whole genome shotgun sequence.
ACCESSION   DASWNS010000560 DASWNS010000000
VERSION     DASWNS010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489998"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VGB76_19385"
     CDS             complement(<1..532)
                     /locus_tag="VGB76_19385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101097.1"
                     /translation="MGKKMIAGLLFLSCCCAATFAFTQEKRRSEKKTNQEHISINGRS
                     LSWEHRNSNDGRTVTLAGKNAQFNDDYTDIERITEGGYFRLTEERDGLVQRLEITPEV
                     GGQLRRLYTVRGEVRPFDGAARQWLARLLSESVAGSGFDAEARVAKVFKRGGANAVLA
                     EIARLKTDHARRVYSSL"
     gene            complement(658..3084)
                     /locus_tag="VGB76_19390"
     CDS             complement(658..3084)
                     /locus_tag="VGB76_19390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0101098.1"
                     /translation="MGTILQDLRYALRMLWKNLGFTGVAVVALALGIGANTTIFSAVN
                     ALLLNPFDFTDADSLVVVWERPPNTRYRNSVSPANFLDVREQQTTFSHVAIYNSNSVN
                     LTEGDKPERLEVAAVSPEMFNALDVRAAVGRTFMKEEEQEGRDKVAILTHDFWRRRYA
                     SDPAILNRTLMLNGQPYTVVGIMPEDFNFPPNNSDIMLPFVITDKLREERGNHYARML
                     GRLKPGVSFAQANADLGAIAQRLEAQYPDTNSNRTIAVESLKADYVRGPRPGLIVLLG
                     AACFVLLLACANVANLLLVRAASRQKEIAIRMALGASRFRLVRQLLTESVLIALIGGA
                     LGLLVAVWGIDLMRVGIPSSLSRYLPGWKNVGIDAQVFWFTLGVSILTGIIFGLAPAL
                     QATKTDFNEALKDGGRTSGGGFNRNRLRGVLIVAEVAISLTLLIGAGLMIKSFYEILK
                     VEPGFKSDSVLVADVALPRKKYEEETARVNFYQQAVERVGALPGVQQAGATNVLPLSR
                     SNNDSAFSVVGRPLPDKGREPKANFRTISPHYLGAMNIPLRRGRYILESDRADTQPVV
                     VINEFLAQQHFPNEDPLGKRLNFGGEEESDNLEIVGIVGNIRHESLVENTVPEVYVPL
                     TQNPWYSMSIVVRAAGDPLPLAAAVQREIQAIDKDQPIYNVRTMERVVSESLAPQRVV
                     MGMLGIFALIALVLASVGIYAVMSYAVTQRTHEIGIRMALGAQPRDILRMVVRQGMLL
                     AITGVALGLVASYWLMQAMSRILYGVSATDPLTFAGISLLLMLVAFTANYIPARRATK
                     VDPMIALRYE"
     gene            complement(3218..5005)
                     /locus_tag="VGB76_19395"
     CDS             complement(3218..5005)
                     /locus_tag="VGB76_19395"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0101099.1"
                     /translation="MKPRCLLVLLLIFALNQGAFSQWAAASGATPALQSSAANVVAFL
                     NVNVVPLDREGVLANQTVVIENGRISALGAASKIKVPKGALRIEGKNRYLMPGLVDMH
                     AHLYSPLELPLYLANGVTTVYNLNGRPANLLWRAEVEQGKLAGPTIYSCGPTIRTAQK
                     AEEARQLVEEQKRAGYDSIKIYNGVSKEAYDALIAEAQKHGMLYIGHIPRAPGLESVL
                     KAKQAIAHAEEFVYTTFKNNTEDESRIPEVAAQTRDAGVQVVLTFVAYDHIIKQVETL
                     PALLATPEMKYLAPWVRESWGVDVNPYKARLGTQGAALNKSLAFQKKLAKELHRVGVR
                     IMTGTDAMNPGVVPGFSEHEELRHLLDLGFTPFEAIRAATRYPGEFLTKTGEFGTVAT
                     GKRADLLLLDGNPLQDIKRISRPLGVMARGRWMSRAELQRMLDDVQPSYAKQAETAKR
                     LLLEDSAKAFDFLNYNDPTYSLLARSAGEVIIEQGIEKFKKAFEDARAKDQKSLLVQE
                     DFINGLGYYLVGQKKMKEAIDVFKLNVAAYPKSGNTYDSLAETYMNTGDKQRAIEFYQ
                     MALEVEPNYANAKAATELLKKLTSEKQGK"
BASE COUNT          942 a         1518 c         1498 g         1061 t
ORIGIN      
        1 gcaggctcga atagacgcgc cgcgcgtggt cggttttcag ccgcgcgatc tccgcgagca
       61 cggcatttgc gccgccacgc ttgaagacct ttgcgacgcg agcctctgcg tcgaagccgc
      121 tgcccgcgac cgactccgag agtaaacgcg cgagccactg gcgcgccgcg ccgtcgaacg
      181 ggcgcacttc gccgcgcacc gtgtacagcc gcctgagttg cccgccgacc tcaggcgtga
      241 tctccaaacg ctgcactagg ccgtcgcgct cctccgtgag gcgaaagtag ccgccttccg
      301 taatgcgttc gatgtccgtg tagtcgtcgt tgaactgcgc gttcttgccc gcgagcgtga
      361 ccgtcctgcc gtcgttcgaa ttgcggtgct cccaactcag gctgcgcccg ttgatcgaga
      421 tgtgctcctg attcgtcttc ttctcgctcc gacgcttctc ctgcgtgaac gcaaacgtag
      481 cggcgcagca gcaggacaga aacaacagac cggcaatcat tttcttaccc atttcttaaa
      541 cctcaatctt tttaagtgac gagaaagaag agtgatgagt gatgagttaa aacaaaagtt
      601 atatctttga ctcatcactc attactcatc actcttcact tcctgctcgt cactcttcta
      661 ttcgtaacgg agcgcgatca tgggatcgac tttggtggcg cgtcgcgcgg gaatgtagtt
      721 ggccgtgaag gcgacgagca tgagcagcag cgagatgccc gcgaaggtga gcgggtcggt
      781 cgcgctcacg ccgtacagga ttcgcgacat ggcctgcatc aaccagtaag aggccacgag
      841 gccgagtgcg acgccggtga tcgcgagcaa catgccctgc cggacgacca tcctgagaat
      901 gtcgcgcggc tgcgcgccga gcgccatgcg gatgccgatc tcgtgcgtgc gctgcgtgac
      961 ggcgtaagac atcacggcgt agatgccgac cgaggccagc acgagcgcga tgagcgcgaa
     1021 gatgccgagc atccccatca ccacgcgttg cggcgcgagc gattcggaga cgacgcgctc
     1081 catcgtacgc acgttgtaga tcggttgatc tttgtcaatc gcctgaatct cccgctgcac
     1141 cgctgcggcg agcggtaagg ggtcgcccgc cgcgcgcacc acgatgctca tcgaatacca
     1201 cgggttttgc gtcagaggga catagacttc gggcactgta ttttccacca gcgactcgtg
     1261 cctgatgttg ccgacgatgc cgacgatctc taaattgtcc gactcttctt cgccgccgaa
     1321 gttcagacgt ttacccagcg ggtcttcgtt cgggaaatgt tgctgagcga gaaattcgtt
     1381 gatgacgacg acgggctgcg tgtccgcgcg gtcgctctcc agaatgtaac gaccgcgccg
     1441 cagagggatg ttcatcgctc cgagataatg cggcgagatg gtgcggaagt tcgctttcgg
     1501 ctcgcgtcct ttatccggca gcgggcgtcc gaccacggag aaagccgaat cattattgga
     1561 gcgcgagagc ggtagaacgt tggttgctcc ggcctgctgc acgccgggca atgcgcccac
     1621 gcgttcaacc gcctgctggt agaaattcac gcgcgccgtc tcctcttcat acttcttgcg
     1681 cggtagcgcg acatccgcga cgagcacgct gtcggatttg aagcccggct cgactttcag
     1741 tatttcgtag aaactcttga tcatcagccc tgcgccgatc aggagcgtca gggagatggc
     1801 gacttcggcg acgatgagca cgccgcgcag gcggttacgg ttaaagcctc cgcccgaagt
     1861 gcgcccgccg tctttcaacg cttcgttgaa gtccgtcttg gtcgcttgca gtgcgggcgc
     1921 gaggccgaag atgatcccgg ttaaaatcga gacgcccaaa gtgaaccaga agacctgcgc
     1981 atcgatgccg acattcttcc accccggcag gtagcgcgag aggctggagg ggatgcccac
     2041 cctcatcaga tcaattcccc agacggcgac gagcaggccg agcgcgccgc cgatgagcgc
     2101 gatcagaacg ctctccgtca acaactgccg gaccagccgg aagcggctcg cgccgagagc
     2161 catgcgaatc gcgatctctt tctggcgcga ggcggcgcgc acgagcagca ggttggcgac
     2221 gttggcgcac gccagcagca gcacgaagca ggccgcgccg agcagcacga tgaggccggg
     2281 gcggggtccg cgcacgtagt cggccttcaa ggattcgacg gcgatggtgc ggttgctgtt
     2341 agtgtccggg tattgcgctt caagccgctg cgcgatggct cccaggtctg cgttggcctg
     2401 cgcgaaagag acgcccggct tgagccgccc cagcatccgc gcgtaatgat tgccgcgttc
     2461 ttcccttagt ttgtccgtga tgacgaaggg gagcatgatg tcgctgttgt tcggagggaa
     2521 attgaaatcc tccggcatga tgccgacgac ggtgtagggc tgcccgttga gcatcaacgt
     2581 ccggttcagg atggccgggt cggaagcgta acggcgacgc cagaagtcgt gcgtaaggat
     2641 cgccaccttg tcgcgtccct cctgctcctc ttccttcatg aacgtgcgcc ccacagccgc
     2701 acgcacgtcg agcgcattaa acatctccgg cgagacggcg gccacttcga gccgctccgg
     2761 cttgtcgccc tcggtgagat tgacggagtt gctgttatag atggcgacgt gcgagaaggt
     2821 cgtctgctgc tcgcgcacgt cgaggaagtt ggcgggcgag acgctgttgc gatagcgtgt
     2881 attcggcggt cgctcccaga ccacgacgag cgagtcggcg tcagtgaaat cgaagggatt
     2941 cagcaggagc gcattgaccg cgctgaagat cgtcgtgttc gcgccgatgc ccaaagccaa
     3001 cgccaccacc gccacgcccg tgaagcccaa attcttccac aacatccgca gcgcatagcg
     3061 caagtcttgc aggatcgtcc ccatcttcaa gttcctcttt taagtgccaa ctacagtgac
     3121 gagtgacaag tgacaagtga cgagataaga ccactgcttc tctttcactc atcactcatc
     3181 actcattact catcacttct ctttcaactc atcagcatca cttaccttgt ttctcgctcg
     3241 tcaacttctt gagcagttcg gtggcggctt tcgcgttcgc gtaattcggc tcaacttcca
     3301 gagccatctg gtaaaactct atggcgcgct gtttgtcgcc ggtattcatg taggtttccg
     3361 cgaggctgtc gtaggtgttg ccggatttcg ggtaggccgc gacgtttagt ttgaacacgt
     3421 caatcgcctc tttcatcttc ttctggccga cgaggtaata accgaggccg ttgatgaaat
     3481 cctcctgcac gagcagcgac ttttgatctt tggcacgcgc gtcttcgaaa gcctttttga
     3541 atttctcgat gccctgctcg atgatcactt cgcccgcgct tctggctaag agggaatagg
     3601 tcgggtcgtt gtagttgaga aagtcgaagg ctttggcgga atcttcgagg agcagacgct
     3661 ttgccgtctc agcctgtttc gcataagacg gttgaacgtc gtcgagcatg cgttgcagtt
     3721 ccgcccgcga catccagcga ccgcgcgcca tgacgccgag cgggcgcgag attctcttga
     3781 tgtcttgcag cggattgccg tcgagcagca ggagatctgc acgcttgccc gtcgccactg
     3841 tgccaaactc tccggtcttc gttaaaaatt ctccgggata gcgtgtggcc gcgcggatcg
     3901 cttcaaaggg cgtaaagccg aggtcgagca ggtggcgcag ttcttcgtgt tcggagaagc
     3961 ccggcacgac gcccggattc atcgcgtccg tgcccgtcat gatccgcacg ccgacgcggt
     4021 gcagttcctt cgccagtttc ttctggaagg cgagagactt gttcaaggcc gcgccctgcg
     4081 tgcccagtcg cgctttgtaa gggttaacgt caacgcccca actctctctc acccacggcg
     4141 cgagatattt catctccggc gtggcgagga gcgcgggcag cgtctcaacc tgtttgatga
     4201 tgtgatcgta ggcgacgaag gtgagcacca cctgcacgcc cgcatcgcgc gtctgcgcgg
     4261 caacttcggg gatgcggctc tcgtcctcgg tattattctt gaaggtggta tagacgaact
     4321 cttcggcgtg cgcgatggcc tgcttcgctt tgagcacgct ctccaaaccg ggggcgcgcg
     4381 ggatgtggcc gatgtagagc atgccatgct tctgcgcttc cgcgatcagc gcgtcgtagg
     4441 cttccttcga gacgccgtta tagatcttga tcgaatcgta accggcgcgt ttctgttcct
     4501 ccacgagttg cctggcctct tccgccttct gcgccgtgcg aatcgtgggg ccacacgagt
     4561 agatggttgg tcccgcgagt tttccctgtt cgacctcagc gcgccagagc agattcgcag
     4621 ggcgaccgtt caggttgtag acggtggtga cgccgttggc gagatagagc ggcaactcca
     4681 gcggcgaata gaggtgcgcg tgcatgtcca ccagacccgg catgaggtag cggtttttcc
     4741 cctcgatgcg cagcgcgcct ttcggcactt tgatcttcga ggccgcgccg agcgcgctga
     4801 tgcgcccgtt ttcaattacg acagtctggt tcgctaagac tccctccctg tccaacggca
     4861 cgacgttcac gttcagaaaa gcgacgacgt ttgcggcgct actttgcagc gcgggcgtcg
     4921 cgccggaagc tgccgcccac tgagagaaag cgccctgatt caaagcgaag atgaggagca
     4981 agaccagcaa acatctcggt ttcatcaaag ctcacctcc
//
LOCUS       DASWNS010000561        14047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_383907, whole genome shotgun sequence.
ACCESSION   DASWNS010000561 DASWNS010000000
VERSION     DASWNS010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14047
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383907"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VGB76_19400"
     CDS             <1..621
                     /locus_tag="VGB76_19400"
                     /inference="COORDINATES: protein motif:HMM:NF036635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101100.1"
                     /translation="LFCEGKGPFRWVALSGDAEDIRRTDDLALELFPEDATLDRWLRL
                     ARDRVQFQGLPARICWLGYGERAEMGEAINELVKRGDLKAPVAIGRDHLDTGSVASPF
                     RETEAMRDGSDAVADWPILNALLNTASGASWVSFHHGGGVGIGYSLHAGQVCVADGTP
                     EAARRLNRVLTNDPGLGVARHVDAGYEEAEETARAKGINVPMLDAG"
     gene            713..1423
                     /locus_tag="VGB76_19405"
     CDS             713..1423
                     /locus_tag="VGB76_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101101.1"
                     /translation="MTGEEMERAIEFLLQSQANAEARMTAFDARQDRTDQQIAEMGKQ
                     ITEMSKRIQAYADTQSQFIEIVTRSLEGLATIQARTEQKIAETNARTEQRIAETNERI
                     EQLATENKKRGAEMDARIEQLIAENKRRGAETKERIEQLAIASKARGAETDARIEQLI
                     AENRARGAEIDERLDRQVEIAARTDARLDQQAEIAARTDERLDRQAEIAARTDARLDR
                     LAALVERNITGGNGNLEQ"
     gene            1505..1666
                     /locus_tag="VGB76_19410"
     CDS             1505..1666
                     /locus_tag="VGB76_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101102.1"
                     /translation="MVRLAGAGIVAITAHALAERPERIAPQLKHDAVGALLRKANLEE
                     FLKAVGVWK"
     gene            1666..2613
                     /locus_tag="VGB76_19415"
     CDS             1666..2613
                     /locus_tag="VGB76_19415"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEY0101103.1"
                     /translation="MRIETESRRTGEESRRAGVRRTARVFVFHFFALLFIVSACRAQT
                     VVGTSARVEAVKFESKLVGKLLPYNVVLPPLYDAPQSRATKYPVLYLLHGLGGSSGDW
                     VSARAHLAAYAAQYRFIIVTPEGADGWYTDSATVPTDKFETYILEELIPDVERRFRTL
                     TERDARAIAGLSMGGYGALKFGVKHPHRFALAASLSGAFHAASWDANDPSLLAFVKPS
                     IARTYGALDSPTRAANDLFKLFDNLPPERIPTLPYIYFDCGTEDFLIGSNRALSELLL
                     RRKIPHEYRQLPGAHSWPYWDAQVQEVLKLAARRLRAAQ"
     gene            2652..3404
                     /locus_tag="VGB76_19420"
     CDS             2652..3404
                     /locus_tag="VGB76_19420"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY0101104.1"
                     /translation="MNDQVVKDKFAILVDGDNAQPSLLEHVLVEVTKYGRITIKRIYG
                     DWTTPNMNGWKKFLLDLAIQPVQQFRYTMGRNATDNTMIIDALDILHRRLVDGFCIVS
                     SDSDFTRLATRIREDGLFVLGIGDSKTPNSFVKACDVFAYTENLVASLPPLQDTSKQS
                     QPKTPVSTKSPGGLKELFKQAFAVSVANDGWVKLSAFGSTLRRLDPAFDPRTYGYAKL
                     SVMLSKKFSNLIEQRRDDKVFPPGIYVRLKSR"
     gene            3602..4045
                     /locus_tag="VGB76_19425"
     CDS             3602..4045
                     /locus_tag="VGB76_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101105.1"
                     /translation="MIKFISGDILRANSEFIALGVVTGAPEGVGAGAAAKVSSKWPKV
                     QKHLRQFTRGNKFQGGDLFVVAPGKNRPGVIFIAALSEAGHVSASFLNRGLRKLARYC
                     IKHKVESVALPKMAAGLEWEDEVKPLLEKFLEGEKTEFFVYEDQE"
     gene            4091..4870
                     /locus_tag="VGB76_19430"
     CDS             4091..4870
                     /locus_tag="VGB76_19430"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0101106.1"
                     /translation="MLDFAMETAREAGRVLAERFGRASLEVTHKGDIDLVTESDLAAE
                     RLIVERIRSYYPRHDILAEESGTSEHAGAGASAYKWIVDPLDGTTNYAHGYPCFCVSI
                     ALEHEGELLLGVIYDPTRDEMFAAERGAGATLNGRRIRVSEINDLNEAMLCTGYPYDV
                     RGRDNFVRNFKNFILHAQGVRRDGSAALDLAYVACGRFDGFWEEGLRPWDVAAGVILI
                     EEAGGRVSRYDGSPFHIYTPPIMVSNGLVHEAMMRVLALRD"
     gene            complement(4889..6124)
                     /locus_tag="VGB76_19435"
     CDS             complement(4889..6124)
                     /locus_tag="VGB76_19435"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5624..5626),aa:Sec)
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HEY0101107.1"
                     /translation="MMNEKTSRRLLWLTLTLVVLGAFVWVFIPVWMIQPFRPQTPEGL
                     NLSYTLRHWSPVVTLAALALAALLVVLLWRGARRWTKAALVLLLVPVVTVTWFARQNH
                     FEWMFKPLPASAYVAADRVDFVAGDEMVLAIEVKGDAVAYPIRQLAYHHVVEDTVGGL
                     PVVSTYUTLCHTGLVWEATSDGRRLKFHLAGINNQNFIMEDEETGTWWQQVSGEAING
                     PLKGRRLNGVFHDELTFATWKREHPQGRVLRPAENTDAWKKFSENWEAQTGKLPVVTQ
                     ASASDRIAPRAIILGVNLNGAAKAYPLETLERQNLLVDRLNGVPLMVVLGEDKRSVRA
                     FETSVDGRELEFFVNADAGAGWRMFDGETRSEWDFTGRAVSGALAGRQLKKIPVLKDY
                     WFDWKIYNPQTSIYAFTAR"
     gene            complement(6150..6836)
                     /locus_tag="VGB76_19440"
     CDS             complement(6150..6836)
                     /locus_tag="VGB76_19440"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0101108.1"
                     /translation="MSETETSIAQRDKKPSSAGTDEPYALPLWAIRFLRAALRLLFRL
                     LWRARYIGVENVPASGGVIIAANHQTYLDPFWLGVPVPLHARFLAWDKVFDWPVAGRL
                     MRLLGAWPLQVQKGDRRAIRRSLQWLRRGGALVIFPEGGRAFSDGKMQEFKVGAARLA
                     LEAGVPVLPITIRGGHRIWPRDWRYPRLGRLEIVYHPLHHLTPLPGEDTRQAARRETD
                     KLYEIIHSAL"
     gene            complement(6833..7276)
                     /locus_tag="VGB76_19445"
     CDS             complement(6833..7276)
                     /locus_tag="VGB76_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101109.1"
                     /translation="MLKKKRSRIVFPREVLLVEIERHCADENCKARTRIGLTKEEARL
                     YDGFECERCELWQADGLSERDIPDWWEELTITGLDTLRAGKTGASDTPETNRAGEGGG
                     VVARLSDAWRRRSSSSGSANSSSGETGEKRSAPEGAPDDVGDEPV"
     gene            complement(7278..8018)
                     /locus_tag="VGB76_19450"
     CDS             complement(7278..8018)
                     /locus_tag="VGB76_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101110.1"
                     /translation="MNYPGKNRRVRERLKLALPARVQFREDAKTEWTEMTRLHDLTPF
                     GASFSLTRPIDVGRLLHLTLPMPRQLRCFDHVEDQYRVYALVRFTRGQFPTGASAPSF
                     MLGVAFVGKRPPDSYQLDPTKRYEVFEPTAEGGMWMLREQATVGGQIQKKETRLSVPV
                     EVTVELYDERWNITAREETVTENISRRGAAVYSSLQAERGRYVRLTSARYQTSAFAVV
                     RAHRTGSDGIPRLHVEFMDQEWPLEEFG"
     gene            8445..11003
                     /gene="gyrA"
                     /locus_tag="VGB76_19455"
     CDS             8445..11003
                     /gene="gyrA"
                     /locus_tag="VGB76_19455"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY0101111.1"
                     /translation="METLDHNYVNIEEEMRRSYLDYAMSVIIGRALPDVRDGFKPVHR
                     RVLWAMNELGNQHNKAYKKSARIVGDVIGKYHPHGDSAVYDTIVRMAQDFSMRYPLID
                     GQGNFGSVDGDSAAAMRYTEARMSKLTSEILADIEKETVDFQPNYDESLSEPRVMPTR
                     VPNLLLNGSGGIAVGMATNIPPHNLGEIVDATIAVIRKPNIKDEDLLKLVPGPDFPTA
                     GFIYGRAGIRSAYLTGRGIIQMRARAGIDRIGRGTQERDAIIITEIPFQVNKARLIER
                     IAELVNEKKIEGISDLRDESDRNGMRIVVELKRDSVPQVVLNKLYKMTPMQSSFGVIN
                     LAIVEGQPRVLTLKQMLEAFVEFRREVVRRRTEYELRKARARAHILEGLNKAIDALDY
                     IIPLIRNAGSVDEARAWLTGHTERMGEGRSWKGIPTDLTQSGFISKLQKVMRRLEFSE
                     AQAQAILDLQLRRLSALERQKILDEYEGIIKFIAELEEILSNENSLRRVIVNELEEVR
                     KNFGDKRRTEIVDEGVELTIEDMIADEEVAITITNSGYIKRTPVSSYTRQGRGGKGRF
                     GAAPKNGDFVEHIFTASTHAYIMIFTDNGQVFKLKVHEIPDAPTHARGKAVVNLINIP
                     STRKLAGVVPVKEFSEGRYVVMVTRKGVIKKTPLSEFQNIRTNGINAINIDDGDELLD
                     VVLTDGTKRIFIATHNGLAIRFDESDVRPMGRATRGVRGVDLRKDDYVVSLCAVSADD
                     SERMLSVSEQGFGKQTPITIYRLQSRGGKGVINMKTTPKTGRVVAVFPVEDDSEIMII
                     TQQAKLIRLEASEIRKTGRGAQGVRLIKTDGGDMVTSASLLAATGEEEVIVTAE"
     gene            complement(11116..12588)
                     /locus_tag="VGB76_19460"
     CDS             complement(11116..12588)
                     /locus_tag="VGB76_19460"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0101112.1"
                     /translation="MRKFSAIINSTTRAALLFACLSAAVAQAQQAQPAATDTPAARRV
                     AELVKIINTTDAAATKKFIEENYGGELAGMPLSAHLNFIAQMRDRTRGVEFHSLQESS
                     ATEATALLRRKLTEDWMGLAVSVEANAPHRITGIGMRPPKVPASERTAKKLTDAERAR
                     ELEAYVQRLAEADVFSGAVLLAKDGKVLFEKAYGEASKDFGVANRVDTKFNLGSMNKM
                     FTAVAIAQLAERGKLSFEDSLAKFLPEFPSKEAAQKIKIKHLLTHTSGLGSYFNRKFM
                     ESSRARFRSVSDMMTLAESETLAFEPGAKWAYSNTGMLVLGAVIEKVTGQTYFDYVRE
                     NIYKPAGMTNTDAYELDLVTPNLAVGYQKEYTEGGVRFRNNVFQHVIRGGPAGGGYST
                     VGDLLKFDAALRANKLVGAEYVKQLLSAKPELNSPEYGYGFGVDTKNRIVGHSGGFPG
                     ISSNLDMFLDNGYTAIVMSNYGGASQPVTRKMQELILAGQ"
     gene            complement(12685..13323)
                     /locus_tag="VGB76_19465"
     CDS             complement(12685..13323)
                     /locus_tag="VGB76_19465"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0101113.1"
                     /translation="MVSAITREEVREAILDVTDQLLARYGYRKMTMDDIAQEVGIGKG
                     TIYLHFASKEEVTLSHVDRIVERLKERLWAIARTDADVAIRLRLMLVARVLFRFDSVQ
                     HYTQSLNDLLAALRPKLLARRARYFEEEARVFAEVLEEGAQAGIFNVEDTLTTAFTLL
                     HATNALLPYSLSTTELGEREEIKEKSEQIADLILSGLLREQKATATRRQRRR"
     gene            complement(13462..13602)
                     /locus_tag="VGB76_19470"
     CDS             complement(13462..13602)
                     /locus_tag="VGB76_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101114.1"
                     /translation="MRLALGGVYGQGKAATALKCLALVGCIIGVLQLYRFILFFTTFY
                     TI"
BASE COUNT         2822 a         4361 c         4205 g         2659 t
ORIGIN      
        1 ctcttctgcg agggcaaggg gccgttccgc tgggtggccc tctcaggcga cgcggaagac
       61 atccggcgca cggacgatct cgcgctcgaa ctgttccccg aagacgcgac gctcgaccgc
      121 tggctccggc tcgcgcgcga ccgcgtgcag tttcagggac tccccgcgcg catctgctgg
      181 ctcggctacg gcgaacgcgc cgagatggga gaagccatca acgaactcgt caagcgcggc
      241 gacctgaaag ctcccgtcgc catcggtcga gatcatctcg acacgggttc ggtcgcctcc
      301 ccttttcgtg aaaccgaagc gatgcgcgac ggctctgacg ccgtggccga ctggccgatc
      361 ctgaacgcgc ttttgaacac ggcgagcggc gcatcgtggg tttcctttca tcacggcggc
      421 ggcgtcggca tcggctattc gctccacgcc gggcaagtct gcgtcgccga cggcacaccg
      481 gaagccgcgc gtcgcctcaa ccgcgtgctc accaacgacc ccggcctcgg cgtcgcgcgc
      541 cacgtagacg cgggctacga ggaggccgaa gagacggccc gcgccaaagg catcaacgtg
      601 cccatgctcg acgcaggata aattttgcgg ataatgtttg cgcctcaaag gtaagtcaac
      661 ccatcaagct tgaaggtgat aagaagcgag tttaaagcga ggaaaaaaac ttatgaccgg
      721 cgaagagatg gaacgcgcca ttgagtttct gctccaaagt caggctaacg ccgaggcacg
      781 catgacggcc tttgatgcgc ggcaggatcg gactgaccaa cagatcgcag agatgggcaa
      841 acagatcacg gagatgagca aacgaataca agcctacgca gacacgcaat cccaattcat
      901 cgagatcgtg acgcggagcc tcgaaggtct ggcaacgata caagcgcgca cggagcagaa
      961 aatagccgaa acaaacgcgc gcacggagca aagaattgct gagacgaatg aacgcatcga
     1021 gcaattagcg actgaaaaca agaagcgcgg ggctgaaatg gacgcgcgca tcgagcaact
     1081 aatagctgaa aacaagaggc gaggggctga gacgaaggag cgcatcgagc aactggcaat
     1141 tgcaagcaag gcgcgcgggg ctgagacgga cgcgcgcatc gagcaactaa tagctgaaaa
     1201 cagggcgcgt ggggctgaga ttgatgagcg gctcgaccgg caagtagaga tagccgctcg
     1261 cacagatgcg cggctcgatc agcaagcaga gatagccgct cgtactgatg agcggctcga
     1321 ccggcaagca gagatagccg ctcgcactga tgcgcggctc gaccgtttgg ccgcgttggt
     1381 ggaacgaaac atcaccggcg gcaacggcaa tttagaacag tgacaagtga cgagttgaaa
     1441 gacaagtttt cggttctaac tcgtgacttg tcactcgtca cttcttaata acgcctctca
     1501 aagtttggtg cggctggcgg gcgcgggcat tgtcgccatc acggcgcacg cgctcgccga
     1561 aagaccggaa cgaatcgcgc cgcaactcaa acacgacgcc gtcggcgcgt tgctcaggaa
     1621 ggccaacctc gaagagtttc tcaaggccgt gggggtgtgg aaatagtgcg gatagaaaca
     1681 gagtcgagac ggacgggaga agagtcgaga cgggcgggcg tgcgacgaac ggcgcgcgtg
     1741 tttgtctttc acttcttcgc gctgctcttt atcgtctcgg cgtgccgggc gcaaacggtc
     1801 gtcggcacgt cggcgcgcgt cgaggcggtc aagtttgaga gcaagctcgt cgggaagctg
     1861 ctgccttaca acgtggtctt gccgccactc tacgacgcgc cgcagtcgcg cgccacgaag
     1921 tacccggtct tgtacctgct gcacggactc gggggcagtt ccggcgattg ggtttccgcc
     1981 cgcgcgcatc tcgccgcata cgccgcacag tatcgcttca tcatcgtcac gcccgaaggc
     2041 gcggacggct ggtacacgga cagcgcgacc gtgccgaccg acaagttcga gacatatatc
     2101 ctcgaagaat tgatcccgga cgtggagcgc aggtttcgca cgctcacgga gcgcgacgcg
     2161 cgcgccatcg cggggctttc gatgggcggc tacggcgcgt tgaagttcgg cgtcaagcac
     2221 ccgcacaggt tcgcgctcgc ggcctccctg agcggcgcgt tccacgccgc ttcgtgggac
     2281 gcaaacgacc cctcgctgct cgccttcgtc aaaccctcca tcgcacgcac ctacggcgca
     2341 ctcgatagcc ccacgcgcgc cgccaacgat ctcttcaaac tcttcgacaa cctgccgccc
     2401 gaacgcatcc ccacgctccc ctacatctac ttcgactgcg gcacggaaga ttttctcatc
     2461 ggctccaacc gcgccctctc cgaacttctc ctcagacgca aaatcccaca cgagtatcgt
     2521 caactccccg gcgcgcactc gtggccttat tgggacgcac aggttcagga agtcctcaaa
     2581 ctcgccgccc gccgcctgcg tgcagcacaa tagcagtcag ccgtctgctg ttaggcacag
     2641 gataaaaggt aatgaatgat caggttgtga aagataagtt tgcgatactt gtcgatggag
     2701 acaatgccca gccgtctctg cttgagcatg tcttagtaga ggttacaaaa tacggacgca
     2761 tcacgatcaa aagaatttac ggcgattgga cgacgccaaa tatgaatggc tggaagaaat
     2821 ttcttctcga cctcgccatc cagccggtgc agcaattccg ttatacgatg ggcaggaacg
     2881 ccaccgataa cactatgatc attgatgcct tagacatcct tcacaggcgt ctggttgacg
     2941 gcttctgtat cgtatcgagt gatagtgatt tcacacgtct ggcgacaagg atcagagaag
     3001 atggactttt tgtacttggt attggagata gtaaaactcc caactctttc gtgaaggctt
     3061 gtgatgtttt cgcttatacg gagaacttag tagcgagcct ccccccatta caagatactt
     3121 ccaaacaatc tcaaccaaag actccggtta gtacaaagtc accgggcggg ttgaaagagc
     3181 tttttaagca ggcgttcgcg gtgagtgtcg caaacgacgg gtgggttaag ctgtctgcct
     3241 tcgggagtac tttacgtaga ttagaccctg cctttgatcc cagaacctat ggctatgcaa
     3301 agctctccgt catgctaagc aagaaatttt ccaacctgat tgagcaaaga cgcgatgaca
     3361 aggtatttcc tccggggatt tatgtcaggc ttaagagtcg ataacatata gctcctgagt
     3421 tctgttataa aacctccgac tgatctgaga ttccgaacgc cacccggcaa ctcatccgac
     3481 ccgccgcctc aagagcattc ttttcaaggt tctgcctctg atgtacattg aaggcttgcg
     3541 cccggcgcag gttaatgcaa acgttgcatg cctctctcga cattgaatct ttagagcgac
     3601 aatgatcaaa ttcatctcag gcgatattct gcgcgcaaac tccgagttca tcgcgcttgg
     3661 cgtggtgacg ggtgcgccgg agggcgtggg ggcgggcgcg gcggcgaaag tctcttcgaa
     3721 gtggccgaag gtgcagaagc acttgaggca gttcacgcgc gggaataagt ttcagggcgg
     3781 cgatctcttc gtggtcgcgc cggggaagaa tcgccccggc gttattttta tcgccgcgtt
     3841 gtcggaggcg ggacacgtct cggcgtcgtt cctgaatcgc ggcttgcgca aactggcgcg
     3901 ttattgcatc aagcacaagg ttgagagcgt cgcgctgccg aagatggctg cggggctgga
     3961 gtgggaagac gaagtgaagc cgcttctgga gaaattcctg gagggtgaaa agacagaatt
     4021 cttcgtctac gaagatcagg aatagatgcg cgggaggttg tcgcgcaaca cttaaccgga
     4081 ggagtccgac ttgttagatt tcgctatgga aactgcgcgc gaggcgggac gcgtgctggc
     4141 agagcggttc gggcgcgcgt cgctggaagt gacgcacaag ggcgacattg atctcgtcac
     4201 cgagtcggac ttggcggcgg agcgtctgat cgtcgagcgc atccgcagct actacccgcg
     4261 tcacgacata ctcgccgaag agtccggcac ctccgaacac gcgggcgcgg gcgcgagcgc
     4321 ctacaagtgg atcgttgatc cgctcgacgg cacgacgaac tacgcgcacg gctacccctg
     4381 cttctgcgtc tccatcgcgc tcgaacacga aggcgaactg ctcctgggcg tcatctacga
     4441 cccgacgcgc gacgagatgt tcgccgccga acgcggcgcg ggcgcgacct tgaacgggcg
     4501 gcgcatacgc gtctccgaaa tcaacgacct caacgaagcg atgctctgca cgggctaccc
     4561 ttatgacgtg cgcgggcgcg acaacttcgt gcgcaacttc aagaacttca tcctccacgc
     4621 gcagggcgtg cgccgcgacg gctcagccgc gctcgatctc gcttacgtcg cctgcggtcg
     4681 cttcgacggg ttctgggaag agggcttgcg cccgtgggac gtggccgccg gagtcatcct
     4741 gatcgaagaa gcgggcgggc gggtctcgcg ctacgacggc tcgccgtttc acatctacac
     4801 cccgcccatc atggtaagca acggcctcgt ccacgaagcg atgatgcgcg tgcttgcgct
     4861 ccgcgactaa aggatttcgt taagactttc agcgcgcggt gaaggcgtag atggaggtct
     4921 gcgggttata gattttccaa tcgaaccagt agtctttgag cacggggatt tttttcagtt
     4981 gtcgcccggc gagcgcgccc gatacggcgc ggcctgtgaa gtcccactcg ctgcgcgtct
     5041 cgccgtcgaa catgcgccac cctgcgcccg cgtctgcatt cacgaagaac tccaactcgc
     5101 gcccgtcaac gctcgtctcg aaggcgcgca ccgatctttt atcttcgccg agcacgacca
     5161 tgagcggcac gccattgagt ctgtccacca gcaggttctg tcgctcaagc gtctctagag
     5221 ggtaagcctt cgccgcgccg ttcaggttaa cgccgaggat gatggcgcgc ggcgcgatcc
     5281 ggtcgctcgc gcttgcctgc gtgacgacgg gcaacttccc cgtctgcgct tcccagtttt
     5341 ccgaaaactt cttccacgcg tccgtgttct ccgccgggcg cagcacgcgc ccctgcggat
     5401 gctcgcgctt ccacgtggcg aaggttagtt cgtcgtgaaa gacgccgttc agacgccgcc
     5461 ctttgagtgg tccgttgatg gcttcgccgg acacctgttg ccaccacgtc cccgtctcct
     5521 catcctccat gatgaagttc tgattgttga ttcccgccag gtgaaatttc agacgccgcc
     5581 cgtcgctcgt cgcttcccac accagaccgg tgtgacagag cgttcaataa gttgacacga
     5641 ccggcaaccc gccgaccgtg tcctccacga cgtgatgata ggccaactgc ctgatcgggt
     5701 aagccaccgc atcgcccttc acttcaatcg ccagcaccat ctcgtctccg gcgacgaaat
     5761 caactctgtc cgccgcgacg tatgcgctcg cgggcagcgg tttgaacatc cactcgaaat
     5821 ggttctgtcg cgcaaaccac gtcaccgtca cgaccggtac gagcagcaac acgagcgcgg
     5881 ccttcgtcca acgacgcgcg ccgcgccaga gcagcacgac gaggagcgcg gcgagagcga
     5941 gcgcggcgag cgtgaccacc ggcgaccagt ggcgcagcgt gtaagagaga ttcaatcctt
     6001 ccggcgtttg cggcctgaac ggctgaatca tccagaccgg aataaagacc cacacgaacg
     6061 cgccgagcac gacgagcgtg agcgtgagcc ataaaagacg acgtgatgtt ttctcattca
     6121 tcattcatcg ttcctcgttc atcctttgtc taaagcgcag agtgaatgat ctcgtagagc
     6181 ttgtcggttt cgcggcgggc ggcctggcgt gtgtcttcgc cggggagcgg ggtcaggtgg
     6241 tggagcgggt ggtaaacaat ttcgaggcga ccgaggcgcg ggtaacgcca gtcgcgcggc
     6301 cagatgcgat ggccgccgcg aatcgtgatc ggcagcacgg ggacgcccgc ttcgagcgcg
     6361 agccgcgccg cgcccacctt gaactcctgc atcttaccgt cggagaaagc ccgcccgcct
     6421 tcgggaaaga tgacgagcgc gccgcccctg cgcagccatt gcagcgagcg tcgaatcgcg
     6481 cgccggtcgc ccttctgcac ttgcagcggc cacgcgccga ggagccgcat cagcctcccc
     6541 gcgaccggcc agtcgaacac tttgtcccac gcgagaaagc gcgcgtgcag tgggacgggc
     6601 acgcccagcc agaacgggtc gagatacgtt tggtgattcg cggcgatgat cacgccgccg
     6661 ctcgcgggca cgttctccac gccgatgtaa cgcgcgcgcc acaacaggcg aaagagcagg
     6721 cgcaaggctg cgcgcaagaa gcggatcgcc cacagcggca gcgcgtaagg ctcgtccgtg
     6781 cccgcgctcg aaggcttttt atcgcgctgc gcgatgcttg tttccgtctc gctcaaaccg
     6841 gctcgtctcc cacgtcatcc ggcgcgccct ccggcgcgct tcgcttctcg cccgtctcgc
     6901 cgctgctgct gttcgcgctg ccgctactac tactacggcg tcgccatgca tcgctcaaac
     6961 gcgcgacgac tccgccgcct tcgcccgccc ggttcgtctc cggcgtgtcg gacgcacccg
     7021 tcttgcctgc gcgcagcgta tcgagtccgg tgatggtcaa ctcttcccac caatcgggaa
     7081 tgtcgcgctc gctcaacccg tccgcctgcc agagttcgca acgctcgcac tcgaagccgt
     7141 cgtagagtcg tgcttcttct ttcgtcaggc cgatgcgcgt gcgcgctttg cagttctcgt
     7201 ccgcgcagtg ccgctcgatc tctacgagca gcacttcgcg cggaaacacg atgcgtgatc
     7261 tcttcttctt tagcatctca accgaactct tccagaggcc actcctgatc cataaactcg
     7321 acgtggaggc gcggaatccc gtcgctgccc gtgcgatgcg cgcggacgac ggcgaaggcc
     7381 gaagtctggt agcgcgcgct cgtcaggcgc acgtagcggc cacgctccgc ttgcagcgac
     7441 gaatagaccg ccgcgccgcg ccggctgatg ttctccgtca ccgtctcctc gcgcgccgtg
     7501 atgttccaac gctcgtcata cagttcgacc gtcacctcga cgggcacgct caggcgcgtc
     7561 tcctttttct gaatctgtcc cccgacagtg gcttgctccc tgagcatcca catgccgcct
     7621 tccgccgtcg gttcaaacac ctcgtaacgc ttcgtcggat cgagttgata actgtccggc
     7681 ggacgcttgc cgacgaaagc cacgccgagc atgaaactcg gcgcgctcgc gcccgtggga
     7741 aactgcccgc gcgtaaagcg caccagagca tagacgcgat attgatcttc gacgtgatcg
     7801 aagcagcgca gttggcgcgg catcggcagc gtcaggtgca gcaaccgccc gacatctatc
     7861 ggtcgggtga gactaaaact cgccccgaac ggcgtcaagt catgaagccg cgtcatctcc
     7921 gtccactcgg ttttggcgtc ctcccgaaac tgcacgcgcg ccggcagcgc gagtttcaga
     7981 cgctcacgca cgcgacggtt tttgcccgga taattcatgg tttcagaagg cgattcgagg
     8041 cgccctcgca cctcaatata ttgtgtccgg cgggcgtcgc tcgccgcgcc ccgaaaagag
     8101 gctcgaaacc ctgattttac agggctttta cgccgttcgg acggttgcta aaaggggcgt
     8161 ttcgggctac aatatgtcgt acttcaaacc ggcacttttg caccccggaa aagtaccgtc
     8221 agacaccaat tttcaggtca cttaaaacag ggcgttcagg ttctcgcgcg ggctgtttcg
     8281 gctgctgctc ccgccgccgc tcgaaggtag gcggcgaccc ctgacagttc ccacgcaacg
     8341 cggaaactcg aaagtgcagt gtggcagagg gagtgcggct cggagccctt gaaatcgtcc
     8401 gcacgaacgg acggacgtga gattgtactc ggaaaggtta gttgatggaa acactagatc
     8461 ataattacgt gaatatcgaa gaagagatgc ggcgttcgta tctcgactac gccatgtccg
     8521 tcatcatcgg acgcgcgctg cccgacgtgc gcgacggctt caagcccgtg caccggcgcg
     8581 tgctctgggc gatgaacgaa ctcggcaatc aacataacaa ggcttataaa aagagcgcgc
     8641 gcatcgtcgg cgacgtgatc ggtaagtatc acccgcacgg cgactccgcc gtctacgaca
     8701 ctatcgtccg catggcgcag gacttctcca tgcgctaccc gctcatcgac ggtcagggca
     8761 acttcggctc ggttgacggc gactccgccg ccgccatgcg ctacaccgaa gcgcgcatgt
     8821 cgaagctcac gagcgaaatt ctcgccgaca tcgagaaaga gacggttgac ttccagccga
     8881 actacgacga gtcgctctcc gagccgcgcg tgatgccgac gcgtgtgccg aacctgctcc
     8941 tgaacggttc gggcggcatc gccgtcggca tggcgacgaa catcccgccg cacaatttgg
     9001 gcgagatcgt ggacgcgacc atcgcggtga ttcgcaagcc gaacattaaa gacgaagacc
     9061 tgctcaagct cgtgcccggt ccagacttcc cgaccgccgg tttcatctac gggcgcgcag
     9121 ggatcaggtc ggcctatctc acggggcgcg gcatcatcca gatgcgcgcg cgcgcaggga
     9181 tcgaccgcat cgggcgcggc acgcaggagc gcgacgccat catcatcaca gagattccct
     9241 ttcaggtgaa taaggcgcgg ctcatcgagc gcatcgccga actcgtcaac gaaaagaaga
     9301 tcgaaggcat ctcagacctg cgcgacgagt cagaccgcaa cggcatgcgc atcgtcgtcg
     9361 aactcaaacg cgactcggta ccgcaggtcg tcctgaacaa gctctacaag atgacgccga
     9421 tgcagtcttc gttcggcgtg atcaatctgg cgattgtcga aggccagccg cgcgtgctga
     9481 ccttgaagca gatgctcgaa gccttcgtcg agtttcgccg cgaggtggtg cgccgccgca
     9541 ccgagtacga actgcgcaag gcacgcgccc gcgcgcacat cctcgaaggt ctgaacaaag
     9601 ccatcgacgc gctcgactac atcatcccgc tcattcgcaa cgctggatcg gtagacgaag
     9661 cgcgcgcatg gctcacaggc cataccgagc gcatgggcga aggcagatcg tggaagggta
     9721 tcccgaccga ccttacgcag tcgggcttca tctcgaagct gcaaaaggta atgcggcgtc
     9781 tggagttctc cgaggcgcag gcgcaggcga ttctcgacct gcaactccgt cgcctgtccg
     9841 cgctcgaacg gcagaagatt ctcgacgagt atgaaggcat catcaagttc atcgccgaac
     9901 tcgaagagat tctttcgaac gaaaactcgc tccggcgcgt gatcgtcaac gaactcgaag
     9961 aggtgcggaa aaacttcggc gacaaacggc gcacggagat cgtggacgaa ggcgtcgaac
    10021 tgaccatcga agacatgatc gccgacgagg aagtggcgat cacgatcacg aactccggct
    10081 acatcaagcg cacgcccgtc tcgtcttaca cgcggcaggg gcgcggcggc aagggacgct
    10141 tcggagcggc tcccaagaac ggcgatttcg tcgagcacat cttcaccgct tcgacgcacg
    10201 cctacatcat gatctttacg gacaacgggc aggtcttcaa gttgaaggtg cacgagatac
    10261 cggacgcgcc gacgcacgcg cgcggcaagg ccgtcgtcaa tctcatcaac attccttcga
    10321 cgcgaaaact ggcgggcgtc gtgcccgtca aagaattttc cgaaggccgc tacgtcgtga
    10381 tggtcacgcg caaaggcgtc atcaagaaga cgccgctgtc ggagtttcag aacatacgca
    10441 cgaacggcat caacgccatc aacatagacg acggcgacga actgctcgac gtggtcttga
    10501 ccgacggcac gaagcgcatc ttcatcgcca cgcacaacgg ccttgccata cgcttcgacg
    10561 agtcggacgt gcgcccgatg ggtcgcgcca cgcgcggcgt gcgcggcgtt gatctgcgga
    10621 aggacgatta cgtcgtgagc ctgtgcgccg tctcggcgga cgacagcgag cgcatgctct
    10681 ccgtctccga acagggcttc ggcaagcaga cgccgatcac gatctatcgc ctgcaatcgc
    10741 gcggcggcaa gggcgtcatc aacatgaaga cgacgccgaa gacgggcagg gtggttgccg
    10801 tcttccccgt cgaagacgac tccgagatca tgatcatcac gcagcaggcc aagctcatcc
    10861 gcctcgaagc gagcgagatt cgcaagaccg ggcgcggcgc acagggcgtc cggctcatca
    10921 agacggacgg cggcgacatg gtgacgagcg cgtcgctgct cgcggccacg ggcgaggaag
    10981 aagtaatcgt gaccgccgag taaagcggcg tcacacgcaa caaacagaaa actcccgctc
    11041 tttgatagtg agcgggagtt ttctgtttgc gacttttacg ggcagtcgga agcgagccgg
    11101 gggcgcgtgc ggcgactatt gcccggccag gatcaactcc tgcatcttgc gtgtgacggg
    11161 ttgcgacgcg ccgccgtagt tggacatgac gatggccgtg tagccgttgt cgagaaacat
    11221 gtcgaggttc gaactgatgc cgggaaaacc gccgctgtgg ccgacgatgc ggttcttcgt
    11281 gtccacgccg aagccgtagc cgtattcggg cgagttgagt tcgggcttgg ccgagagcag
    11341 ttgcttgacg tactccgcgc cgacgagttt gttcgcgcgc agcgcggcgt cgaacttcaa
    11401 caggtcgccg accgtcgaat agccgccgcc cgccgggccg ccgcggatga cgtgctggaa
    11461 gacgttgttg cggaagcgca cgccgccttc ggtgtactcc ttctggtaac cgacggcgag
    11521 attgggcgtc acgaggtcta actcgtaagc gtctgtgttc gtcatcccgg cgggcttgta
    11581 gatgttctcg cgcacgtaat cgaaataggt ctgcccggtc actttctcga tgaccgcgcc
    11641 gagcacgagc atccctgtgt tgctgtaagc ccattttgcg cccggctcga aggcgagcgt
    11701 ctcgctctcc gcgagcgtca tcatgtcgct cacgctacgg aagcgcgcac gcgacgactc
    11761 catgaacttc cggttgaagt agctgcccag accggaagtg tgcgtcagca ggtgcttgat
    11821 cttgatcttc tgcgccgcct ctttcgaagg aaactccggg agaaacttcg cgagcgaatc
    11881 ttcaaacgag agcttgccgc gctccgcgag ttgcgcgatg gcgaccgccg tgaacatctt
    11941 gttcatcgag ccgaggttga atttcgtgtc cacgcgattc gcgacgccga aatccttgct
    12001 cgcttcgccg taagccttct cgaagagaac cttgccgtcc ttcgccagca gcaccgcgcc
    12061 ggagaacacg tcggcctccg ccaatcgctg cacgtaggct tcgagttcgc gcgcacgctc
    12121 ggcgtccgtc agtttcttcg ccgtgcgctc gctggcgggg actttcggcg ggcgcatgcc
    12181 gatgcccgtg atgcgatgcg gcgcattcgc ttcgacgctc accgccagac ccatccaatc
    12241 ttccgtcaac ttccggcgca ggagcgcggt cgcctccgtc gcgctgctct cctgtaagct
    12301 gtgaaactcg acgccgcgcg tgcggtcgcg catctgtgcg atgaagttca ggtgcgcgct
    12361 caacggcatc ccggccagtt cgccgccgta gttctcttct ataaacttct tcgtcgccgc
    12421 cgcatccgtc gtgttgatga tcttcaccaa ctctgcgacg cgacgcgccg ccggagtgtc
    12481 tgtcgcggcg ggctgcgcct gctgcgcctg cgcgacggcg gcagacaggc aggcgaacaa
    12541 cagtgcggcg cgggtggttg aatttatgat ggctgaaaat tttctcatct ctcgattgtc
    12601 ctcgtgtgtg tggggcgcgc tcgtcgcgcg tttaattctc gcattcaccg gcggcggacg
    12661 cgtgcgcggt cgcacgttcg tgctttagcg gcgacgctgc cgacgggtcg cggtggcttt
    12721 ctgttcccgg agcagtccgc tcaggatcag gtctgcgatc tgctcgctct tttctttgat
    12781 ctcttcacgc tcgcccagtt cggtcgtgct caggctgtag ggcaggagcg cgttggtggc
    12841 gtgcagcagg gtgaaggcgg tcgtgagcgt atcttccacg ttgaagatgc ccgcctgcgc
    12901 gccctcttcg agcacctccg caaagacgcg cgcctcctct tcaaaataac gcgcgcgccg
    12961 cgccagcaac ttcgggcgaa gcgcggccag caggtcgttc aaactctgcg tgtaatgctg
    13021 cacgctgtcg aaacgaaaga gcacgcgcgc caccagcatc aggcggaggc gaatcgctac
    13081 gtcggcgtcc gtgcgtgcaa tcgcccacaa acgctccttg agccgttcga cgatgcggtc
    13141 aacatgcgac agcgtgacct cttccttgct cgcgaagtgc aggtagatcg tgcccttgcc
    13201 gatgccgact tcctgcgcga tgtcgtccat cgtcatcttg cgatagccgt agcgcgccag
    13261 cagttggtcg gtcacgtcga gaatcgcctc gcgcacctct tccctcgtga ttgcgctgac
    13321 cataatttcc tgacctctga ccggaattgc tttccagtca taattacgcc gtgaccgaca
    13381 agtagttcca aactttttcc cgcccgcgag aatcggcgga aaaaatgaga gcatttctta
    13441 gatgaacttc aggtgggcgg ttcagatggt gtagaacgtc gtgaagaaga ggatgaagcg
    13501 atagagttgg aggacgccga taatgcagcc gacgagcgcg agacatttca gggcagtcgc
    13561 cgccttcccc tgcccgtaga ccccgccgag cgcgagtcgc aaagctgcgg aagagattcg
    13621 actcgaaact cgtcaggata ttgaacgcct cgcccagaaa gcgtcggagc gagtagtctt
    13681 cgctctcgat tcgacgctcg ccgcaattgg gacagaagcg gctgcgaaag ctttcgccgc
    13741 agttgggaca agtgaggtca gacgcatcgc ggggcgacgg ttcgcgggac ggcagcgacc
    13801 ggggcggcgc ggcatcgccg gtcgcttcgt ccaccagcgc gatgtcggtc ggttccggca
    13861 tgttgcgcgt cgaacgaaat ttgcacgggt ggggtcagtt cattccggcg acggcttgct
    13921 ggtatttcgc cgcgagttcg tcctgattcg tgatgcagat gtccaactcg cgcacgaggc
    13981 cgtcgccgag tccgtaaatc caaccgtaca cgcccaactc ctgccctcgc tcccaggcgt
    14041 tctgcac
//
LOCUS       DASWNS010000562         6864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_306290, whole genome shotgun sequence.
ACCESSION   DASWNS010000562 DASWNS010000000
VERSION     DASWNS010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306290"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VGB76_19475"
     CDS             <1..287
                     /locus_tag="VGB76_19475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0101115.1"
                     /translation="TLLLRARNEGAGVRIEVCDTGIGIPQENLARIFDPFFTTKEVGQ
                     GTGLGLAVCYGIVTEHGGRVTVDSVLGRGTTFTLVLPSMSVERFDGGRMS"
     gene            284..1723
                     /locus_tag="VGB76_19480"
     CDS             284..1723
                     /locus_tag="VGB76_19480"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0101116.1"
                     /translation="MSNTPQEELTNTQPSRILVAEDEANLRLVLQKELQRQGHDVRVA
                     PDGEAALRLLEESNVDVLLCDINMPRMDGLELLRRVHMRPNPPEVIMLTGHATVETAI
                     EAMKLGAYDYLSKPYHINELDTLVKRAAEKRRLRVDNQRLRAQLARHSEMPEIVHVSD
                     AMREALRLVERVAPSDASVLITGESGTGKELIAHAIHRLSQRREASFIDLNCAAFQES
                     LLESELFGYEAGAFSGAKGRKLGLFELADNGTLFLDEVTELPAPLQSKLLRAIETQTF
                     YRVGAVRKVQVNVRIIAATNRDMTQAVSDGSFRADLLYRINGFQINLSPLRERPEDIE
                     PLSRHLVKQLGGLNPPEITPEALAALSAYPWPGNVRQLRNCLERALLLANDARITTNE
                     LPPEIANPAASAFVPGFTGATPAAAGGSAPVEGATAVQPPLREVERQQIVSALEQTNW
                     HRGKTAEILGISPSTLYRRLRDYNLTRRS"
     gene            complement(1843..2979)
                     /locus_tag="VGB76_19485"
     CDS             complement(1843..2979)
                     /locus_tag="VGB76_19485"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101117.1"
                     /translation="MRFSKVIHFTCACALLFSSSMPLRASSLDADVRYMATSAPPVPS
                     SASPEVREESATETATAQAANAGAKKRPNLFVRIITAPFRGLAKLFGSGGDKTARVKA
                     KEANPAQAQTATTAAANESGNGALPVVATPRAAVVSPVVVPQMRASEAADESADYEPR
                     RFTPFIESAPGDPLSQGRALLESGYTNEAIAELSIAAVAGPAGTDLVEANNLLGLAYD
                     RRGQHKQAQEFYERALSVAPLNPRVLNNLGHSLYLDDRYNESLSRLKTAARLAPADTQ
                     VANNLALVYGRLGKFDEVFKQFKRAGGEFYARTNTAALLAVAGRDRDAIKHYEAARKL
                     NPASSLVLRQLITLYVRTGQREKAEAAERALDKVPASTGAATSS"
     gene            complement(3276..4022)
                     /locus_tag="VGB76_19490"
     CDS             complement(3276..4022)
                     /locus_tag="VGB76_19490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0101118.1"
                     /translation="MKKLLIGIALIAVFSLHGTAFADTITFTAPPTAPDAAGSAGSNP
                     GGPNQFDLEHHHAYTWQINAAGRIPAGHTITSATLTFSSITNWDTNPNTLFVRMFDSA
                     NSFGSASGSRSATVNGVTSIQDVDPNQVPVTSISDYFAGSNALVNNTGTVIGQFTDTN
                     GVNTTQNVVFTFNQAQLAALISYLNNGSNLAFGFDPDCHFWNNGITFTIKTSPPSAVP
                     EPATMTLLGTGLAGLYYRRRRQQQQRQQQA"
     gene            complement(4236..4649)
                     /locus_tag="VGB76_19495"
     CDS             complement(4236..4649)
                     /locus_tag="VGB76_19495"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY0101119.1"
                     /translation="MPFSTRITVRFGDTDPAGLVYYPNIFHYFHIALEEFFGARCGIS
                     YQQLMAVERIGFPNVNTQTEFFVPIVYGDELDLEIFVSQTGNSSATFQYSARRASDGT
                     LCARSTQIHVAMNLDTRRPVSIPEKYRQAFAGSAR"
     gene            complement(4769..6322)
                     /locus_tag="VGB76_19500"
     CDS             complement(4769..6322)
                     /locus_tag="VGB76_19500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEY0101120.1"
                     /translation="MNIEFPERFNMADYFLYHNVEEGREGKVCLYFEDERYTYAHAVR
                     MANCAGNALAREGGVELEDRVLIVLPDCPEFVWTWFGASRIGAVITMVNPLLPAADYV
                     YYLEYTRARVAVIHESLLETFTEAARGARHLRRVFVVGREHGEFASFGEVTAAMPDEL
                     TPADTHRDDIAIWLFTSGSTGHPKGAVHLQHDLPFNTECYAKRVLGVNEHDLTVSVPK
                     LFFGYATGTNLLFPFAVGGATALFAERSTPEKLFEVIARYRPTILTSVPTMINGMLNV
                     EGAAARDLSSLRFCLSAGEALPVELYERWMETFGVEILDGIGSAEMFHIYITNRPNDV
                     KPGSLGRIVEGYEAQIIDAAGVPVATGEMGTLRISGDSAALCYWQAHEKSKETFAGDA
                     CTTGDQFHVDAEGYYWYHGRTDDMLKVSGVYVAPVEIEACLLQHEAVLECAVIGHDAG
                     DRLVKPKAFIVPRENFAPTKELAENVKEFVKTHLARYKYPRWIEFVASLPKNDRGKID
                     RKKLKDSDK"
     gene            complement(6408..>6864)
                     /locus_tag="VGB76_19505"
     CDS             complement(6408..>6864)
                     /locus_tag="VGB76_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101121.1"
                     /translation="FRSIVYTSYGVLSWLPSLDRWAEVVAHFLKPGGFFYIAEFHPFI
                     FMLGDDGHTFEYPYFHTPEPLKLHSTGSYAAPNAPGFSHTEYNWSHSLADVVNAVIRA
                     GLRLEFLHEFPYQPVVPSPAEKFEPGLTRLEGWQVEPPHIFSLRALKEK"
BASE COUNT         1354 a         1983 c         2200 g         1327 t
ORIGIN      
        1 ggacgctcct gttgcgcgcg cggaacgaag gcgcgggcgt gcgcattgag gtgtgcgata
       61 cagggatagg gataccgcag gagaatctgg cgcgcatctt cgatcccttc ttcacgacga
      121 aggaggtcgg gcagggaacg ggtcttgggc tggcggtctg ttatggtatt gtgacggagc
      181 acggagggcg cgtcacggtg gattcggtgc tcgggcgcgg aaccactttt acgctggtct
      241 tgccctcgat gagtgtcgag cgatttgacg gaggccgaat gagttgagta acacgccgca
      301 agaggagttg acgaacacac agccgagcag gattttggtg gccgaagacg aagcgaacct
      361 gcgcctcgta ttgcaaaagg agctgcaaag acaggggcac gacgtgcgcg ttgcgcccga
      421 cggggaagcc gccctgcgcc tgctcgaaga gagcaacgta gacgtgctgt tgtgcgacat
      481 caacatgccg cgcatggacg gactcgaact gttgcgccgc gtgcacatgc gaccgaaccc
      541 gcccgaagtg atcatgctga ccgggcacgc taccgtcgag acggccatcg aagcgatgaa
      601 actcggcgcg tacgattatc tctccaagcc ttatcacatc aacgaactcg acacgctcgt
      661 caaacgcgcg gcggagaagc gtcgcctgcg cgtggacaat caacgcctgc gcgctcaact
      721 cgcgcgacat tccgagatgc cggagatcgt ccacgtctcg gacgcgatgc gcgaggcttt
      781 gagattggtc gaacgcgtcg cgccgtcgga tgcgtcggtg ctgatcacgg gcgagtcggg
      841 cacgggcaag gaactgatcg cgcacgcgat tcaccgcctc tcgcagcgca gagaagcgtc
      901 gtttattgat ctcaactgcg ccgcgtttca ggagtcgctc cttgaatcgg aactcttcgg
      961 ctacgaagcg ggcgcgttct ccggcgcgaa ggggcgcaaa ctcggactct tcgaactcgc
     1021 agacaacggc acgctctttt tagatgaagt cacggaactg cccgcgccct tgcagtcgaa
     1081 gctgttgcgc gccatcgaga cgcagacttt ctacagggtc ggcgcggtgc gcaaagttca
     1141 ggtcaacgtg cgcatcatcg cggcgacgaa tcgagacatg acgcaggccg tctccgacgg
     1201 aagttttcgc gccgacctgc tctaccgcat caacgggttt cagatcaact tgtcgcccct
     1261 gcgcgaacgc cccgaagaca tcgaaccgct ctcacgccat ctcgtcaagc aactcggcgg
     1321 cctgaacccg ccggagatta cgcccgaagc cctcgccgcc ctgagcgcat acccctggcc
     1381 gggcaacgtg cgccaactgc gcaactgcct tgagcgcgcc ctgctgctcg ccaacgatgc
     1441 gcgcatcacg acgaacgagt tgccgccaga gatcgctaac ccggcggcga gcgcgtttgt
     1501 tccgggcttc accggcgcga cgcctgccgc cgcagggggc agcgcgcccg tcgaaggcgc
     1561 aaccgcagtg cagccgccgc tgcgcgaagt ggaacgtcaa cagatcgtct ccgcgctcga
     1621 acagaccaac tggcatcgcg gcaagaccgc cgaaatcctc ggcatctcgc cctccacgct
     1681 ttatcgtcgc ctgcgtgatt acaatttgac gcggcggagt taaaccccta agcaataatc
     1741 cgaacacaca cgtagacgca gaaacgaggc ggataaagac cattccttat ccgcctcgtt
     1801 ctgtctccgc ctgcgtctgt ctctatcttt gcagaactgc ttttaactgc tggtggctgc
     1861 gcccgtgctg gcaggcacct tgtcgagcgc gcgttcggcg gcctccgctt tttcgcgttg
     1921 cccggtgcgc acgtagagcg tgatgagttg gcgcagcacg agagatgagg cggggttgag
     1981 tttgcgcgcg gcttcgtaat gtttgatcgc gtcgcggtcg cgcccggcca cggccagcag
     2041 cgcagccgtg ttcgtgcgcg cgtagaattc gccgcccgca cgcttgaact gtttgaagac
     2101 ttcgtcgaac ttgccgagcc gcccgtagac gagcgcgaga ttgttggcga cctgagtatc
     2161 tgcgggagcg agccgcgcgg cagttttcag acgcgagagc gattcgttgt agcggtcgtc
     2221 gaggtagagc gagtggccaa ggttgttgag cacgcgcggg ttgagcggcg caaccgagag
     2281 cgcgcgttca taaaattcct gcgcctgctt gtgctgcccg cgacggtcgt atgcgagtcc
     2341 gagcaggtta ttggcctcga ccagatcagt gccagcagga ccggcgacgg cggcgatgga
     2401 cagttccgca atcgcctcgt tcgtgtagcc gctctccaac agcgcgcgac cctgcgagag
     2461 cggatcgccc ggcgcgcttt caatgaacgg cgtgaacctg cgcggctcat aatcggcaga
     2521 ctcgtccgcc gcttcggaag cgcgcatctg cggcacaact acgggcgaca cgactgctgc
     2581 gcgcggcgtt gccacgacgg gaagcgcgcc gtttcctgat tcattggctg ctgctgtggt
     2641 agccgtctgc gcttgcgccg ggttcgcttc tttcgctttc acgcgtgccg tcttgtcgcc
     2701 accgctgccg aaaagtttgg cgaggccgcg aaagggtgcg gtgatgatgc gtacgaaaag
     2761 gttcgggcgt ttcttcgccc ccgcgttcgc cgcctgcgcc gtcgcagtct cagttgccga
     2821 ttcctcccgc acctccggcg acgcgctcga cggcaccggc ggcgcgctcg tcgccatgta
     2881 gcgcacgtct gcgtcgaggc tcgacgcgcg cagcggcatt gatgaagaga agagcagcgc
     2941 acaggcgcag gtgaaatgaa tgactttaga aaaacgcacg gcagcagaat cctttcgcag
     3001 acagtttgca cgcatccggg gggcgcgaac tcgacggcca cacgggcgcg tgcaacttca
     3061 cggagattga atcttagggc aggcgaaggc tcaggtcaaa agctcacagc cgggttgcgt
     3121 ccggacaggg agcgggaacg caaaagcacg gacgggatgg ttaccgtccg tgcttttgtg
     3181 agtaagagag agcaagagag actaagggtg caagagagcc gggcttaagc gagtcgggct
     3241 tgggagagcc gttgtttgtc ggtgcgtttg aactgttatg cctgttgttg gcgttgctgc
     3301 tgttgcctgc gtcggcggta gtagagtccg gccaagcctg tgccgagcag cgtcatcgtc
     3361 gcaggctcgg gcactgccga gggcggagag gtcttgatgg tgaacgtgat gccgttgttc
     3421 cagaagtggc aatcggggtc gaagccgaac gcgagattgc tgccgttgtt gagataggag
     3481 atgagcgcgg ccaactgcgc ctgattgaag gtgaagacga cattttgcgt cgtgttcacg
     3541 ccgttcgtat ccgtgaactg gccgatcacc gtgcccgtgt tgttgacgag cgcgttgctg
     3601 ccggcgaagt agtcgctgat ggaagtgacg ggcacttgat tcggatcgac atcctgaatc
     3661 gaggtcacgc cgttgacggt tgccgagcgc gaaccgctgg ccgagccgaa gctgttggcc
     3721 gagtcgaaca tgcgcacgaa gagcgtgttg gggttggtgt cccagttggt gatactgctg
     3781 aaggtgagcg tcgcgctggt gatggtgtgg ccggcgggaa tccttccggc ggcgttgatc
     3841 tgccacgtgt aggcgtggtg atgttcgaga tcaaattgat tcgggccgcc cggattcgaa
     3901 cctgcgctac cggcggcatc gggcgcggtc ggcggcgcgg tgaacgtgat ggtgtctgcg
     3961 aaggccgtgc cgtgcagact aaagacggca atcagggcga taccgatgag caactttttc
     4021 acgacgggtt gaacctcctg cggcaactta accgggtttg aatagacgga ctacgacgtg
     4081 gcctgcaaag agaatgtctt tgaggttgca ggcgattaaa aaacttaacc ttttccttgt
     4141 cgcccttgaa gtaaatgact agccccgcct cttttccata aaactcacgc aagcgaagca
     4201 attgtctgtg aggccgggca caaccgcgcc atctctcagc gtgcgcttcc ggcaaaggct
     4261 tgacgatatt tttcgggaat ggagacgggg cggcgcgtgt cgaggttcat cgcgacgtgg
     4321 atttgcgtag agcgggcgca cagagttccg tcgctcgcgc gccgcgcgct gtactggaag
     4381 gtggcggaac tgttgcccgt ctgcgagacg aagatttcca ggtccagttc gtcgccatag
     4441 acgatgggaa cgaagaactc tgtctgcgtg ttgacgttgg gaaagccgat gcgctcgacg
     4501 gccatcaatt gttgataact gatgccgcag cgcgcgccga aaaattcttc gagggcgatg
     4561 tggaagtagt gaaagatgtt cgggtaatag acgagtcccg ccgggtctgt atcgccgaag
     4621 cgcacggtga tgcgtgtgga aaatggcatg agaaagcagt gccgaatgac aggtgacaag
     4681 tgacgggaga agagaagtag ataagtgatg agtgatgagt gatgggtgat gagttgaaga
     4741 cagggctttt gttttaactc gtcacccgtc acttgtcact gtccttgagt ttcttgcgat
     4801 caatcttgcc gcgatcattt ttcgggagcg aggcgacaaa ctcgatccag cgcgggtatt
     4861 tgtagcgcgc caggtgcgtc ttgacgaact ctttgacgtt ctcggccaac tctttggtcg
     4921 gcgcaaaatt ttcgcggggc acgatgaagg ctttcggttt aacgagacgg tcgcccgcgt
     4981 cgtgtccgat gacggcgcat tcgagcaccg cttcgtgttg cagcaggcag gcttcaattt
     5041 cgacgggcgc gacatagacg ccggagactt tcagcatgtc atccgtgcgc ccgtgatacc
     5101 agtagtagcc ttccgcatcg acgtgaaatt gatcgccggt cgtacacgca tcgcccgcga
     5161 aagtctcctt cgatttctcg tgcgcctgcc agtagcagag cgcagccgag tcgccggaga
     5221 tgcgcagcgt ccccatctcg cccgtcgcca ccggaacgcc tgccgcatca ataatctgcg
     5281 cctcgtagcc ttcgacgatg cgccccagac tgccgggctt cacatcattc gggcggttcg
     5341 tgatgtaaat atgaaacatc tccgccgagc cgatgccgtc cagaatttcc acgccgaacg
     5401 tctccatcca acgctcgtac aactcgacgg gcagagcctc gcccgccgac aggcagaagc
     5461 gcagactcga caggtcgcgc gccgccgcgc cttccacgtt gagcatgccg ttgatcatcg
     5521 tcggcacgct cgtcaggatc gtcgggcggt aacgtgcgat cacttcgaac aacttttccg
     5581 gcgtcgaacg ctccgcgaag agagcggttg cgccgcccac tgcgaagggg aacagaaggt
     5641 tcgtgcccgt cgcataaccg aagaacagct tcggcacgga gacggtcagg tcgtgctcgt
     5701 taacgccgag cacgcgcttc gcataacatt cggtgttgaa cggcaggtcg tgttgcagat
     5761 gcaccgcgcc tttcggatgc cctgtggaac cggaagtgaa caaccagatc gcgatgtcgt
     5821 ctcgatgcgt gtcggcgggc gtgagttcat ccggcatcgc cgccgtcact tccccgaacg
     5881 acgcgaactc gccgtgctcg cgcccgacga cgaacacacg gcgcaggtga cgcgccccac
     5941 gcgcagcctc cgtgaaagtc tccagcagcg attcgtgaat gacggccacg cgcgcacgcg
     6001 tgtattcgag gtaatagacg taatcggcgg caggcaagag cgggttgacc atcgtgatca
     6061 ccgcgccgat acgactcgcg ccgaaccacg tccacacgaa ctcagggcaa tccggcagga
     6121 cgatgagcac gcggtcttcc aactccacgc cgccttcacg cgcgagcgca ttccccgcgc
     6181 agttcgccat ccgcacggcg tgcgcatacg tgtatcgctc gtcctcgaag tagaggcaca
     6241 ccttgccttc gcgcccttcc tccacgttgt gatagaggaa gtagtcggcc atgttgaagc
     6301 gttcgggaaa ttcgatgttc atatccggcg atctctaatc aaactaaaga cggaagttga
     6361 tcttgcgacg cgctcataag actacagcaa acttcatctc ctgctcacta cttttccttc
     6421 aaggcgcgga gcgaaaagat gtgcggcggc tcgacctgcc agccttcgag ccgcgtcagg
     6481 ccgggttcaa acttttccgc cggggagggg acgactggct gatagggaaa ctcgtgcagg
     6541 aactcaaggc gcaagcccgc gcggatgacg gcgttgacca cgtcggccag actgtgcgac
     6601 cagttgtact cggtgtgcga gaagcccggc gcgttcggcg cggcgtagga gccggtcgag
     6661 tgcagcttga gcggttcggg cgtgtggaag taagggtact cgaaggtgtg tccgtcgtcg
     6721 ccgagcatga agatgaacgg gtggaattcg gcgatataga agaagccgcc gggcttgagg
     6781 aagtgtgcga cgacttcagc ccagcggtcg agactgggca gccaggagag cacgccgtag
     6841 gaggtgtaca cgatagatcg gaag
//
LOCUS       DASWNS010000563        17002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_514206, whole genome shotgun sequence.
ACCESSION   DASWNS010000563 DASWNS010000000
VERSION     DASWNS010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17002
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514206"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VGB76_19510"
     CDS             <1..375
                     /locus_tag="VGB76_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101122.1"
                     /translation="ERAGGAGAESSNAAKNVAPAATATTVAQTQSSRTAQAAAREARK
                     GVSSAASVTAAEAAPGTPRGGAYVVRVLMEVKNGRVTDARVLNPRPGAGAYEALALRM
                     ARQRRYPENFSGGDTLRISVKP"
     gene            461..1954
                     /locus_tag="VGB76_19515"
     CDS             461..1954
                     /locus_tag="VGB76_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101123.1"
                     /translation="MLFIQSNSLSLYLDEGARLRLLDALRERGLTPLIRSARRRCFGL
                     LVPEQEEAARVEAAIAEFERECVAQHEAANRMKTGDATSYTPGEIFCAGDAVLYLTFG
                     ASAASPQGLTTGIVYSIETTEPLTKLDRFCRDVREALIKTEKRSEGGENAAEAELSEL
                     PCWKTRATRSTQGLARFIAIQPTDFAVAAKLKGGAREFVRASELLEEASVRQFLRRVQ
                     EMRREGYSPRRLFAEAATLRGGVSVERMFDAGLLEREIRVSCRKSGHALFDLPTPDSL
                     AAITISKAKCSLCAAPVADEVVEETINPTRLAVSLLEDGGWLNNRVYKIIRSLGVPES
                     EIATGPASTHGESYLAADVCGNSYLFVTRDGDLTPAFSRRIVETIAETEATHLVVVLT
                     GKVEDEGRLRLYEYAWRRARNGQDLDTTLIEDLESAHAEIERSFERAVHRQLSRHLFT
                     LDAALGFSASNFVLDWFKLKHASATTGNRASNLLVPVFPDIDQRVAS"
     gene            complement(1983..3008)
                     /locus_tag="VGB76_19520"
     CDS             complement(1983..3008)
                     /locus_tag="VGB76_19520"
                     /inference="COORDINATES: protein motif:HMM:NF006159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0101124.1"
                     /translation="MKVNEAKKKSLAGKIAIVAGATRGAGRGIACMLGEAGATVYCTG
                     RSVRGKPPTSGVHAGRTETIEETAEMVTAHGGVGLHARVEHTDEAQVAALFERVRAEQ
                     RGRLDILVNVLGGDTQAEWKPFWQQPLDKGLRWLSTEVFSHIITSRYAASLMLARKRK
                     GGLIVVITDGDGVGYRGSFFFDLVKISTIRLAYALGEELAPDGIASVAISPGFMRTEW
                     MLEHFGATEENWREVAETNPEAKSFGFIASETPCFVGRAIAALAADPRVMDKSGGVYG
                     SWTLSEEYGFTDVDGNRPHMGRHLEEHFKEMLNAPPKTGFRWKLERDTPPVQARRPAR
                     VKRATRR"
     gene            3187..4164
                     /locus_tag="VGB76_19525"
     CDS             3187..4164
                     /locus_tag="VGB76_19525"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEY0101125.1"
                     /translation="MRADRLFSIVLLLQSHRHLTSRDLAGRLEVSARTIHRDMEALSG
                     AGIPVVAERGTGGGWSLMGEYRTNLTGLNEAEIKSLFVSKPSRLLADLNLEKAAEGAF
                     LKLLAALPSVYRRGAEHARQRIYVDPTGWNRAEEAVPLLHVLQEAVWQERRARIRYGR
                     GECDAAIERTVDPLGLVAKGSVWYFVAAVEGGDVRSYRVSRVEGVELLDEQIVRPPDF
                     DLAAFWEQSSAQFRAHLPAYRARVRVRREIVARMPYAGRFARIEETGEPDAEGWVEVA
                     LRFDAEELACEYALGFGTQLEVLEPPSLREKVLAAAQNVVASYTQKTAR"
     gene            complement(4349..5473)
                     /locus_tag="VGB76_19530"
     CDS             complement(4349..5473)
                     /locus_tag="VGB76_19530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY0101126.1"
                     /translation="MVVKIDDLLRGATASGASDLHIKAGSFPVMRVDGELKPVTNAAR
                     LTQDDTLDMAFSIMSNRQKQRFKEVSEVDIAYGLSGVGRFRANVFQQRGTVSMVLRVI
                     PDKVGTMTELGLPPTVEQIGNEQRGLILVTGATGSGKSSTLAAIIDHINSTRGGHIVT
                     IEDPIEFLHRDKKSFISQREVDVDTRSFAEALRGALRQDPDVILVGEMRDHETIETAL
                     TAAETGHLVLSTLHTLDATETITRIVSSFPPHQQKSVRLQLAGILRAVISMRLVRASN
                     GVGRVPAAEIMISTGTIRDYIANEEKTPLIRDAIAAGTSQYGMQTFDQSLFNLFQSGL
                     ISLEEALRGASNPDEFRMRVSGICTSNDQAKEHIVSMSNQ"
     gene            complement(5829..6920)
                     /locus_tag="VGB76_19535"
     CDS             complement(5829..6920)
                     /locus_tag="VGB76_19535"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY0101127.1"
                     /translation="MFADDAPAKDIKTPRINPEIWHNGQFIKWDDARVHVMSHVLHYG
                     SSVFEGIRCYDTKRGPAIFRLREHMRRLMNSAKIYRMDHDFTLDQLCDAAVELVQRGS
                     LEQCYLRPIIFRSLDEARPAFGVNPFPNPLVCYIGAWDWGKYLGDEAIEAGVDVCVSS
                     WNRLSPNSMPAMAKSGANYMNSQLIKMEAIKNGYSEGIALDDRGFVSEGSGENIFLVH
                     DGKVITPPLSSSILPGITRDSVIQICRELDIPVAETGLQRAALYVADELFFTGTAAEV
                     TPIRSVDKIKIGTGKRGEITARIQQAFFEVTKGERAAPGDWLTYVKEAKASAVPQGNN
                     GHAAAPDRKATSNATEPELSESFLTVGTE"
     gene            7184..8500
                     /locus_tag="VGB76_19540"
     CDS             7184..8500
                     /locus_tag="VGB76_19540"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY0101128.1"
                     /translation="MRYRRMNVSMFVLASFALACLTLEGCRMSQNSNATPPVAVSSAT
                     PPIANTTTDTATGRVSREDADDPRAVHQRAILIDMHADTVQRMIDEGADINMRLPDGH
                     LDAARMHEGGLDAQFFSIWVEPQYFGGGGKSAVERADKQIAAVRALAEEHPETWELAT
                     TAADVRRIAAAGRLAALMGLEGGYAIDERLENVEKYYQLGVRYMSPAWSVSLSWAGSS
                     GDKIGETRGLNEFGRKVIAEMNRLGIMVDVSHVSDRTFWDIIETSTKPVIATHSGVRS
                     IVNVPRNLTDDQLRALARTGGVCSVVFYPRFIEPGWQEAQGKVDAEIAPLVAEASRRA
                     TGTPVQKRLARDRVREREYAKRLPPVSVARIVDHIDHIVKLVGVDHVGIGSDFDGIQA
                     VPHDLSNVADFPNLTAELLKRGYTAEDVNKILGCNILRVLEAVAGK"
     gene            8599..10812
                     /locus_tag="VGB76_19545"
     CDS             8599..10812
                     /locus_tag="VGB76_19545"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0101129.1"
                     /translation="MNPFTLLGILLGYVKPYTSRALLLVATLLLEGTFNILLALSLKF
                     IIDLAITPRDANVLALILGGLAAGFLLTAASQVVRDYLYAWLGARILNDIRKEMFRHL
                     QQLSVGFYARSRMGDLLSRFSSDLAAIENAVVLGVPGALLCVINIIFSTCVLFALDWR
                     LALVAMLGLPLCIVGPKIFAPRALRVGYTLRGEQATMSSTIQENLGAQQIVKAFSLKK
                     SVLANFEGQADKVARLATRFNFLSYVTERSPNIGMLLFNVLLISGGSYLAYRGLLSIG
                     SLVSFNALFITVSTSVMGLTAVTPTLLQATSGMQRVRELLDERPAVVEPANARHLPPL
                     SSGIKFEDVTFGYTSAQNNLVGVSMNLPKGARIAFVGHSGCGKSTNLNLIMRFYDPQT
                     GRVLFDGIDLREAALDSLYSQVGIVFQESFLFNTSVRENIRLGKSGATDGEVEAAARA
                     AELEEIILKMPEGYDSIVGERGGRLSGGQRQRVAIARALIRNPSLIILDEATSALDPA
                     TEAAINETLERVSRGRTVISVTHRLKSVIDYEHIFVFREGSIIEHGTHESLLRRGESY
                     AAMWRRQNGASVSANGDLRFTDTGILRDVPLFADLDEPYLKEISELFITARVPAARTI
                     IEEGDEGYRFYIIIHGKVAVTVADDEGNTLHVATLEDGDYFGEIALIADIPTTATVVT
                     LTPCIFLILQREHLHKLMRQHAGFGAQLRDALTRRMAETEAISDAPGGTHYAGKS"
     gene            10796..11743
                     /locus_tag="VGB76_19550"
     CDS             10796..11743
                     /locus_tag="VGB76_19550"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEY0101130.1"
                     /translation="MPENLEDSLRGVPAGAKRRPGLTGEARVRSRPKIGIHAPIAGGL
                     QNALVKARQLNCDTVQIFSRNPRGWQARPLPEDEVETFRRVRGENGITPVVIHANYLI
                     NLAAADEVIREKSVASFREEVERGLTLGADYLVVHPGSAKGACEVDGVRTCGESLRAA
                     CAGLDLGTFRVLLENTAGQGACIGHRFEHLRDIIAACPALDLGVCFDTAHAFTAGYDI
                     REEDGLAATFDALERNVGTANIRAVHFNDSKADYNSRVDRHWHIGLGHIGAPALARVA
                     AHPKLSDAAFILETPQDEHGDDERNLAALRAFVTADARL"
     gene            11913..12545
                     /locus_tag="VGB76_19555"
     CDS             11913..12545
                     /locus_tag="VGB76_19555"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY0101131.1"
                     /translation="MAKRGTIVIDPGHGGTVEVGGSSHNNARSPSGVLEKNLTLRMAF
                     HVRDALAEAATIGGHTIKILLTRETDKNVGLRDRALFAKNNNADLFLCIHFNASVAHN
                     ARGVETLISPKAGNSNHTADKAFAQLIQTATFNAIKAHDAGTKDRKVKDQSLAVLRES
                     SLGTKARGCLVEMEFLDTKAVDELFNLGANAPKVRSDVAQGIANALIAAL"
     gene            12953..15511
                     /gene="leuS"
                     /locus_tag="VGB76_19560"
     CDS             12953..15511
                     /gene="leuS"
                     /locus_tag="VGB76_19560"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY0101132.1"
                     /translation="MDEKYFPEKIEEKWQRVWAETGAFEVEPDDARPKFFCLEMLPYP
                     SGFLHMGHVRNYSIGDALAWHKRLQGFNVLHPIGWDSFGQPAEQAAIKRGVQPREWTE
                     ENIAHMRGQFKRLGISFDWRREMAAHRPDYYRWDQWFFLQMLARGLVYRRTSAINWCP
                     KEETVLSNEQSSGGVCWRCGTPVEKRDLEQWFIRTTEYAEALLADMKEIEAGWPERVL
                     TMQRNWIGQSIGAYIDFPIKDTEAKVRVFTTRIDTIYGATAIVVATEHPLLNELEESP
                     LKDKVMAFVERVKAERASRAAMPGEEVEKEGVNTGLFAVNPFSGEQIPVWVANYVLIE
                     YGAGAVMAVPAHDERDYEFAQKYSLPVRRVIERVSHEQESIPHNEASGLDQSTAMPHA
                     FTDYGILVNSAEWGGRLSKDALREMAAHAEAKGFGGGAVTYRLRDWGISRQRFWGAPI
                     PIIYCDACGIVPVPETDLPVVLPERAEFTGAGNSPLANVKEFVNTTCPKCGQAARRET
                     DTMDTFVDSSWYFFRYCDPKNTDAPFDKARVAYWTPVDQYIGGIEHAVMHLLYTRFWT
                     KMMRDIGLVTFDEPVRNLLTQGMVTNVVEGTGEWKAMSKSLGNGVDPDEMIEAYGADA
                     VRLFVMFAAPPENELRWLETGIEGAVRFLRRVYNFVWRWRASLTDALSKEAAGALEFN
                     DASRALRRKTHQTIARITQDFSELHFNTSIAALMELLNALGEFEVKPEAATEADKFAV
                     REALEALVSMLAPFTPHVAEELWEALGHAGGLLGSGARWPEADAELARKEALEIPVQI
                     NGKLRGRIHATPDTSEEELRTAALAEEKVRALIEGRQIVKVIVVPQRLVNIVVR"
     gene            15706..16437
                     /locus_tag="VGB76_19565"
     CDS             15706..16437
                     /locus_tag="VGB76_19565"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0101133.1"
                     /translation="MTSEQPASEPNKKLLTGARAALAALTFSLLAIALSSSCNPTDST
                     HNGSRNDPPPAATPAAGSNPATAPPGTGAPPTQPARSTSPAPLPEAIANAAMTTLDGK
                     PLRLSDLKGKVVILNLWATWCGPCRREIPDFIKIQEDYNGRGVEVLGITSEDERNTAE
                     MVKEFVKEFEINYKVVWVDQETWGEFLAPRYSIPQTLVLDQNGQLLQKFVGYSPQVAV
                     MAREIADRALSGATESGGRTDQTAK"
     gene            complement(16623..16865)
                     /locus_tag="VGB76_19570"
     CDS             complement(16623..16865)
                     /locus_tag="VGB76_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101134.1"
                     /translation="MSIDPDKVQTQEETGEPAAIDEVEGRVQAEMKIAEGRAKESVAQ
                     GLADKELEREGRELQEEGERELEQERQNSDSGSGGE"
BASE COUNT         3232 a         5209 c         5425 g         3136 t
ORIGIN      
        1 gagcgtgcgg gcggcgcggg tgcggaatcg tcgaacgcgg cgaagaatgt tgcgcccgcc
       61 gccacagcga cgacggtggc gcaaacccag tcgtcccgca cggctcaggc ggctgcgaga
      121 gaggcgcgca agggtgtctc ttccgccgcc tctgttaccg ccgcagaggc cgcgccgggc
      181 acgccgcgcg gcggcgcata cgtcgtgcgc gtgttgatgg aggtgaagaa cggacgggtg
      241 acggatgcac gcgtattgaa cccgcgaccc ggcgcgggag cctatgaagc gctcgcgctc
      301 aggatggcgc ggcagcgtcg ctacccggaa aatttcagcg ggggcgatac gctcaggatc
      361 agtgtcaaac cgtaagggaa aactccgcgt gcttggttga aggttggcac gcgacgcgca
      421 gaccgggcac atgcgcggcg ggcgcggatg gaatcaaaag atgctgttca ttcaaagcaa
      481 cagtctgtct ctctacctcg acgaaggcgc acgcctgcgc ctgctcgacg cgctcaggga
      541 acgtggactc acgccgctca tccgctcggc gcgtcgtcgt tgcttcgggc tgctcgtgcc
      601 cgaacaggaa gaggccgcgc gcgtcgaagc ggccatcgcg gagtttgaac gcgaatgcgt
      661 ggcgcagcac gaggcggcga acagaatgaa gacgggcgac gcgacatctt acacgccggg
      721 cgagatattc tgcgcgggcg atgccgtgct ctatctcacc ttcggcgcga gcgcggcctc
      781 gccgcagggc ttgacgacgg gcatcgtcta cagcatcgag acgacggaac cgctcacgaa
      841 actcgaccgc ttctgccgcg acgtgcgcga ggctttgatc aagacggaga agcggagcga
      901 gggcggcgag aacgcggcgg aagcggagtt gtccgaactg ccttgctgga agacgcgcgc
      961 gacgcgttcg acgcaagggc tggcgcgctt catcgccatt caaccgaccg attttgccgt
     1021 cgcggcgaag ttgaagggcg gcgcgcgtga gttcgtgcgc gcctcggagt tgctggagga
     1081 agcgtctgtc agacaatttt tgcggcgcgt gcaggagatg cggcgtgagg gttattcgcc
     1141 gcgtcgcctc ttcgcggagg cggcgacgct caggggcggc gtctccgtcg aacggatgtt
     1201 cgacgcggga ctgctggaac gcgagatcag ggtcagttgc cgcaagtcag gccacgccct
     1261 cttcgacctg ccgacgcccg actcgctcgc ggcgatcacg atcagcaagg cgaagtgcag
     1321 cctgtgcgcc gcgcccgtcg cggacgaagt ggtcgaagag acgatcaacc cgacgcggct
     1381 ggccgtgtcg ctgctggaag acggcggctg gttgaacaat cgcgtctaca agatcatacg
     1441 ctcgctcggc gttcccgaaa gcgagatcgc tacgggtccc gcttccacgc acggcgagtc
     1501 gtatctggcc gcagacgtgt gcggcaactc ttatctcttc gtcaccagag acggagactt
     1561 gacgcccgcc ttctcccgcc gcatcgtcga gacaatcgcg gagacggagg cgacgcatct
     1621 cgtcgtcgtc ctcacgggca aggtcgagga tgaaggtcgg ctgcggctct acgagtacgc
     1681 gtggcggcgc gcacgcaacg ggcaggattt agacacgacg ctcatcgaag accttgagag
     1741 cgcgcacgcc gagatcgaac gctctttcga gcgcgccgtt catcgccagc tatcccgaca
     1801 cctgtttacg ctcgacgccg cgctcggctt tagcgcgagc aacttcgtgc tcgactggtt
     1861 caagttgaag cacgcttccg ccaccacagg caatcgcgcc tcgaaccttc tcgtgcccgt
     1921 cttcccggac atagatcaac gcgtcgcctc ctgaagtcac cgcttgaagt caccgcttga
     1981 agtcaccttc gagtcgcacg cttcacacgt gcggggcgtc tggcctgaac gggcggcgtg
     2041 tcgcgttcga gtttccagcg aaagcctgtc ttgggcggcg cgttgagcat ctccttgaag
     2101 tgctcttcga gatgtcgccc catgtgcggg cggttgccgt caacgtccgt gaagccgtac
     2161 tcctccgaca gagtccagga gccatagacg ccgcccgact tgtccatcac gcgcgggtct
     2221 gcggcgagcg cggcgatggc gcgcccgacg aagcacggcg tctccgatgc gatgaagccg
     2281 aagctcttgg cttccgggtt cgtctcggcc acttcgcgcc agttctcctc cgtcgcgccg
     2341 aagtgttcga gcatccattc cgtgcgcatg aagccgggcg agatggcgac cgacgcgatg
     2401 ccatccggcg cgagttcctc gcccaacgcg taggccagac ggatcgtgga aatcttgacg
     2461 aggtcgaaga agaaactgcc gcgatagccg acgccgtcgc cgtccgtgat gacgacgatg
     2521 aggccgccct tgcgtttgcg cgccagcatc agcgaggcgg cgtagcggct cgtgatgatg
     2581 tgcgaaaaaa cctctgtgct gagccagcgc aaacctttgt cgagaggttg ttgccagaaa
     2641 ggcttccact cggcctgcgt gtcgccgccg aggacgttga cgaggatgtc gagccgcccg
     2701 cgctgttcgg ctcgcacgcg ctcgaagagt gcggcgacct gcgcttcgtc cgtgtgctcg
     2761 acgcgtgcgt gtagccccac gccgccgtgc gccgtgacca tctccgccgt ctcttcgatg
     2821 gtttccgtgc gcccggcatg aacgcccgac gtgggcggtt tgccgcgcac gctgcgcccc
     2881 gtgcagtaga cggtcgcgcc cgcttcgccc agcatacagg cgatgccgcg ccccgcgccg
     2941 cgagtcgccc ctgcgacaat cgcaattttg cccgccagac tctttttctt cgcttcgtta
     3001 actttcatct ttcccgcccc cctgcgcttc gtcggttgca gacgcaatca agtctccgcc
     3061 ttgcaccagc tttctccccg cgagataaat tcaggataga cgcggattga tgacaccctc
     3121 tgtcatattt ctgagataaa atcgcgcggt ctttaagatt ttgcgggcgg agtgaggacg
     3181 gacgggatgc gcgcggatcg actcttttcc atagtgctgt tattgcagtc tcatcgtcat
     3241 ttgacctcgc gcgatctggc gggacgtctg gaagtctccg cgcggacgat tcaccgcgac
     3301 atggaagcac tgagcggggc ggggattccg gtcgtcgcgg agcgcggcac gggcggcggg
     3361 tggtcgctca tgggcgagta tcgcacgaac ctgacggggc tgaacgaggc agagattaaa
     3421 tcgctcttcg tcagcaagcc ttcacgcctg ctggccgact tgaatctgga gaaggccgcc
     3481 gaaggagcgt ttttgaaatt gctggccgcg ctgccttccg tctacaggcg cggggcggag
     3541 cacgcgcgcc aacgcatcta cgtagacccc acgggctgga atcgcgcgga ggaagccgtg
     3601 ccgctgcttc atgtgctgca agaagccgtc tggcaggagc ggcgcgcgcg catacgctac
     3661 gggcggggcg agtgtgatgc ggctattgag cggacggtcg atccgctcgg actggtggcg
     3721 aaggggagcg tctggtactt cgtggcggcg gttgaaggcg gagacgtgcg cagctatcgc
     3781 gtctcgcgcg tcgagggcgt ggagcttttg gacgaacaga tcgtgcgtcc gccggatttc
     3841 gatttggcgg ccttctggga acagtcgtcg gcgcaatttc gcgcgcacct gcctgcctat
     3901 cgcgcgcgcg tgcgtgtgcg ccgtgagatc gttgcgcgga tgccttacgc cggaaggttc
     3961 gcgcgcatcg aggagacggg cgagccggat gcggagggct gggttgaagt cgcgctcagg
     4021 ttcgacgcgg aagagttggc gtgtgaatac gcgctcggct tcggcacgca gttggaagtt
     4081 ttggagccgc cgtcgctgcg cgagaaggtc ttggccgccg cgcagaacgt cgtcgccagc
     4141 tacacgcaga agacggcgcg ataagaatcg ccgcttcgat tgaagcggtt gaaaatgatt
     4201 ttgacgaaga accaagactc gccggagagc aaaacgatga aggcggaacg atgagcgatg
     4261 aaggagaaaa cagaattcag gagtcagaag caagagacgc gcacgtttta ttcatccttc
     4321 atcgctcatc gttttgaact ctctgacttc actggttact catgctgacg atatgttctt
     4381 tcgcttgatc gttgctcgtg cagatgccgg agacgcgcat gcggaactcg tccgggttcg
     4441 acgcgccgcg cagggcttct tcgagcgaga tgaggccgga ttgaaagaga ttgaagagcg
     4501 attggtcgaa agtctgcatg ccgtactgag aagtccctgc ggcgatggca tcgcggatga
     4561 gcggcgtctt ttcctcgttg gcgatgtaat cgcggatcgt gccggtcgag atcatgatct
     4621 cggcggcggg cacgcgcccc acgccgttcg aggcgcgcac gaggcgcatc gagatgacgg
     4681 cgcgcagaat gcctgcgagt tgcaggcgga cgctcttttg ctggtgcggc gggaaggacg
     4741 agacgatgcg cgtaatggtt tcggtggcgt cgagcgtgtg gagcgtggag agaacgaggt
     4801 ggccggtttc ggcggcggtg agggcggttt caatcgtttc gtggtcgcgc atttcgccga
     4861 cgagaatcac gtccgggtct tggcgcagcg cgccgcgcag ggcttcggca aaggagcgcg
     4921 tgtccacgtc aacttcgcgt tgcgagatga aggatttttt gtcgcggtgc aggaactcta
     4981 tcgggtcttc gatggtgacg atgtggccgc cgcgcgtcga gttgatgtgg tcgatgatgg
     5041 cggcgagcgt ggaggatttg ccggagccgg ttgcgcctgt gacgaggatg aggccgcgct
     5101 gttcgttccc gatctgttcg acggtgggcg gcaatccgag ttcagtcatg gtgcccacct
     5161 tgtccgggat gacgcgcaag accatgctca ccgtgccccg ctgctggaag acgttggcgc
     5221 gaaagcgacc gacgcccgac agcccgtacg cgatgtcaac ttccgagact tctttgaagc
     5281 gttgcttctg gcgattcgac atgatcgaga aggccatgtc gagcgtgtcg tcctgcgtga
     5341 gtcgggcggc gttggtgacg ggcttcagtt cgccgtccac gcgcatgacg ggaaacgaac
     5401 cggctttaat gtgcaggtcg gacgcgccgc tcgcagtcgc accccgcagg agatcatcaa
     5461 tcttgactac cataaaatga acgggaacag attcagtcgg atcaaatttg agcgcgtgcg
     5521 attgccgacg gacgcacacg ggcacaggct acgcggcttt taaactacgc gcttcgtcgg
     5581 aattgaaaac gattggctgg cttcgcgggt tgatgcggag ggattttgcg ccacgggaaa
     5641 gattagcaac ccgaagacgc cggaaacatt ctagcgagca gcgcagggcg cgtcaatgaa
     5701 tatctctccg gcgcgcaaag cggcgcgcgc tgacgttaaa agcgaagcct ccgaagggct
     5761 gcgagagctt gaagaaacgc tttcgtcgcc gttcggaggc ttcgtgtctg actgccgaaa
     5821 ttccgtcgtt attccgtgcc gaccgtgagg aaactttcgg acagttccgg ttcggtcgcg
     5881 ttcgaagtgg ctttcctgtc cggcgcggcg gcgtggccgt tgttgccctg cggcacggcg
     5941 gacgccttcg cttctttcac gtaagtgagc cagtcgcccg gcgcggcgcg ctcgcccttc
     6001 gtcacctcga agaaggcttg ctggatgcgt gcggtgatct cgccgcgctt gcccgtgccg
     6061 atcttgatct tatcgacgga gcgaatcggc gtcacttccg ccgccgtgcc cgtgaagaac
     6121 agttcgtcgg cgacgtagag cgcggcgcgt tggaggccgg tttcggcgac gggaatatcg
     6181 agttcgcggc agatctggat cacgctgtcg cgggtgatgc ccggcagaat ggaagaggag
     6241 agcggcggcg tgatcacctt gccgtcgtgt acgaggaaga tgttttcgcc cgaaccttcc
     6301 gagacgaaac cgcgatcatc gagcgcgatg ccttcggagt agccgttctt gatggcttcc
     6361 atcttgatga gttgcgagtt catgtagttt gcgccggact tcgccatcgc gggcatcgag
     6421 ttgggcgaga ggcggttcca cgaagagacg cacacgtcca cgcccgcttc aatcgcttca
     6481 tcgccgagat acttgcccca atcccacgcg ccgatgtagc agacgagcgg gttcggaaac
     6541 ggattgacgc cgaaagcggg tcgcgcttcg tcgagactgc ggaagatgat cgggcgcagg
     6601 tagcactgtt cgagcgagcc gcgctgcacc agttcgaccg ccgcgtcgca caactggtcg
     6661 agcgtaaagt catggtccat gcgatagatc ttcgcggagt tcatcaaacg ccgcatgtgc
     6721 tcgcggaggc ggaagatggc ggggcctcgc ttggtgtcgt aacagcggat gccctcgaag
     6781 acgcttgagc cgtagtgcag gacgtgcgac atcacgtgga cgcgtgcgtc gtcccacttg
     6841 atgaattgac cgttatgcca gatttcggga ttgatgcgcg gcgtcttaat atctttggcg
     6901 ggtgcgtcgt ctgcaaacat tgatttgctc ctcttcgtct gtccgcgctg aagtgcggcg
     6961 gcggcgaaat taaaattcga ttgatgccat ttaaagaaat ggatttgtag aaacagtgcc
     7021 taatttgtgc gcggtagatt aaatcgcaca ataacaggaa acgagcgcgg cttgcaagcg
     7081 cgccccttgc tactttcgac gcccgcgaat taccatcttt gccgtcttaa taaacgttga
     7141 ttcaaagcga aattccgcac ccggttctgt gagcaaaaga gcgatgcgat accggcgaat
     7201 gaatgtctcg atgttcgtgc tcgcgtcctt tgcgctcgcc tgtctcacgc tcgaaggttg
     7261 tcgcatgtcg caaaactcaa acgccacgcc gcccgtcgcc gtctcatcgg cgacaccccc
     7321 aatcgcaaac acaaccacag acacggccac aggcagagtc tcgcgcgaag acgcggacga
     7381 cccgcgcgcc gtgcaccaac gcgccatcct catagacatg cacgccgaca cggtgcagcg
     7441 catgattgac gaaggcgcgg acatcaacat gcgccttccc gacggccatc tcgacgccgc
     7501 gcggatgcat gaaggcggac tcgatgcgca gttcttctcg atctgggtgg agccgcaata
     7561 cttcggcggg ggcggcaagt ctgccgtgga acgcgcggac aaacagatcg ccgccgtgcg
     7621 cgcgctggcc gaagagcacc cggagacgtg ggaactggcg acgaccgccg cagacgtgcg
     7681 acgcatcgcc gctgccgggc gactcgccgc gctgatgggg ttggaaggcg gctatgcgat
     7741 tgacgagcgt ttggagaacg tggagaagta ttaccaactc ggcgtgcgct acatgtcgcc
     7801 cgcgtggtct gtctcgttga gttgggctgg ctcgtcgggc gataagattg gcgagacacg
     7861 cggcctgaac gagtttggac gcaaggtgat cgcagagatg aatcgcctcg gcatcatggt
     7921 cgatgtctcg cacgtctcgg atcggacgtt ctgggacatc atcgaaactt ccacgaaacc
     7981 cgtgatcgcc acgcactccg gcgtgcgctc catcgtcaac gtcccgcgca acctgacgga
     8041 cgaccagcta cgcgcgctcg cacgcacggg cggcgtctgc tccgtcgtct tctacccgcg
     8101 cttcatcgag ccgggctggc aggaggcgca gggaaaagtt gacgcggaga tcgcgccgct
     8161 cgtggcggag gcttcgcgcc gcgccaccgg cacgcccgtg cagaagagac tcgcgcgcga
     8221 ccgcgtgcgc gaacgcgaat atgcgaagcg tctgccgcca gtctccgtcg ctcgcatcgt
     8281 cgatcacatc gatcacatcg tcaaactcgt cggcgtcgat cacgtcggca tcggctcgga
     8341 cttcgacggc attcaagccg tcccgcacga cctctcgaac gtcgcagact tcccgaacct
     8401 gaccgccgaa ctcttaaagc gcggctatac cgccgaagac gtaaacaaga ttctcggctg
     8461 caacatcctg cgcgtgttgg aagcggtcgc ggggaagtag gcgggcgagc gcgcgcgagc
     8521 aaaactgtcc tcttccgaag atgccggaga cacatgccga gcgagactgt agaaaattcc
     8581 gttggttcca gggcggcgat gaaccccttc acgctcctcg gaatcctgct cggctacgtt
     8641 aagccttaca cctcgcgcgc gttgctcctc gtcgccacac tgttgctcga aggcacgttc
     8701 aacatcctcc tcgcgctcag tctgaaattt atcattgacc tcgccatcac tccgcgagac
     8761 gcgaacgtgc tcgcgttgat tctcggcggt ctggccgcag ggtttctgct cacggcggcc
     8821 tcgcaggtcg tccgcgatta tctctacgcg tggctcggcg cacgcatcct caacgacatc
     8881 cgcaaggaga tgtttcgcca cctccagcaa ttgtctgtgg gcttttacgc gcgctcgcgg
     8941 atgggcgatc tgctgtcgcg cttctcgtcc gatctggcgg cgattgaaaa tgcggtggtg
     9001 ctcggcgtgc cgggcgcgtt gctgtgcgtg atcaatatta ttttcagcac gtgcgttctg
     9061 ttcgcgctcg attggcggct cgcgctcgtc gccatgctcg gcttgccgct ctgcattgtt
     9121 ggccccaaga ttttcgcgcc gcgcgcgctc agggtgggct acacgttgcg cggcgagcag
     9181 gccacgatgt cgagcacgat tcaggagaac ctcggcgcgc aacagatcgt caaagcgttc
     9241 agtctcaaaa agtctgtact ggcgaacttc gagggacagg ccgacaaggt tgcgcgtctg
     9301 gcgacgcgct tcaacttcct cagctacgtc accgagcgca gcccgaacat cgggatgctg
     9361 ctcttcaacg tgctgctcat cagcggcggc tcttatctgg cttacagggg gctgctctcc
     9421 atcggctcac tcgtctcttt caacgcgctc ttcatcacgg tcagcacctc cgtcatgggt
     9481 ttgacggcgg tgacgccgac gctcctgcaa gcgacgagcg ggatgcagcg cgtgagggaa
     9541 ctactcgacg agcggccagc cgtcgtcgaa ccggcgaacg cacgccatct gccgcctctc
     9601 tcttcgggga tcaaattcga ggacgtgacc ttcggctaca cctccgcgca gaacaatctc
     9661 gtcggcgtca gcatgaatct cccgaagggc gcgcgcatcg ccttcgtcgg tcatagcggc
     9721 tgcggcaaga gcaccaatct caacctcatc atgcgctttt acgacccgca gaccgggcgc
     9781 gtcctgttcg acggcataga cctgcgcgag gccgcgctcg attcgcttta tagtcaggtg
     9841 ggcatcgtct ttcaggagag ttttctcttc aacacgagcg tgcgcgagaa catacggctc
     9901 ggcaagtcgg gcgcgacgga cggcgaagtc gaagcggcgg cgcgtgcggc ggaactcgaa
     9961 gagatcattc tcaagatgcc cgaaggctac gacagcatcg tcggcgagcg cggcgggcga
    10021 ctctccggcg ggcagcgtca gcgcgtcgcc attgcgcgcg cgctgattcg caacccgtcg
    10081 ctgatcattc tcgacgaagc gacctccgcg ctcgaccccg cgacggaggc cgccatcaac
    10141 gagacactgg aacgcgtctc gcgcgggcgc accgtcatct ccgtcacgca caggctcaag
    10201 tccgtgatcg actacgagca catcttcgtc ttcagagagg gcagcatcat cgagcatggg
    10261 acgcacgaga gcctcttgcg tcgcggtgaa agttacgcgg cgatgtggcg gcgacagaac
    10321 ggcgcgagcg tctcggcaaa cggcgacctg cgcttcacgg atacaggcat cctccgcgac
    10381 gtgcccctgt tcgcagacct cgacgagcct tatctgaaag agatctccga gttgttcatc
    10441 acggcgcgcg tccccgccgc gcgcacgatc atcgaggaag gcgacgaagg ctaccgtttc
    10501 tacatcatca ttcacggcaa ggtcgccgtc accgtcgcgg acgacgaagg aaacacgctc
    10561 cacgtcgcca cgctcgaaga cggcgactac ttcggagaga tcgcgctcat cgcagacatc
    10621 ccgaccaccg ccaccgtcgt cacgctcacg ccctgcatct ttctcatctt gcagcgcgaa
    10681 catctacata aactcatgcg ccagcacgcc ggattcggcg cacaactgcg cgacgcgctc
    10741 acacgccgga tggcggaaac cgaagccatc agcgacgcac cggggggaac tcactatgcc
    10801 ggaaaatctt gaagacagtc tgcgcggggt gcctgcgggc gcgaaacgcc gacccggcct
    10861 gacgggcgag gcgcgtgtgc gaagccgccc gaagatcggc atccacgcgc ccatcgcggg
    10921 cgggcttcag aacgcgctcg tcaaggcgcg acaactgaac tgcgatacgg tgcaaatctt
    10981 ttcgcgcaac ccgcgcggct ggcaggcgcg cccgctcccg gaagatgagg tcgagacttt
    11041 cagacgcgtg cgcggcgaaa acgggatcac gcccgtcgtc attcacgcca actacctgat
    11101 caaccttgcc gccgcagacg aagtgatacg cgagaagtcc gtcgcttcgt ttcgcgaaga
    11161 ggtggagcgc gggctgacgc tcggcgcgga ttatctggta gtgcatccgg ggagcgcgaa
    11221 gggcgcgtgt gaagttgacg gcgtgcggac gtgcggggag agtctgcgcg cggcctgcgc
    11281 cggactcgac ctcggcacgt ttcgcgtcct gctggaaaac acagcggggc aaggcgcgtg
    11341 catcggccat cgcttcgagc acctcaggga catcatcgcc gcctgtcccg cgctcgacct
    11401 cggcgtctgc ttcgacaccg cgcacgcctt caccgccggt tacgacatac gcgaggagga
    11461 cgggctggcc gccaccttcg acgcgctcga acgaaacgtc ggcaccgcca acatccgcgc
    11521 cgtacacttc aacgactcga aggccgatta caactcgcgc gtggatcgtc actggcacat
    11581 cggcctcgga cacatcggcg cacccgccct cgcacgcgtc gccgcgcatc cgaaactctc
    11641 agacgccgcc ttcattctcg aaacgccgca ggacgagcac ggcgacgacg agcgcaacct
    11701 cgccgcgctg cgcgctttcg tcaccgccga cgcgcgcctt tgatgaccgg acgcgggcgg
    11761 cgcgcttcaa tgtagttgga aaatttcttg acagccgccg gagtttcttc tattcaagta
    11821 cgcctcgcac gtcttttcaa agttcagcag ccgcaccgtt cttgctcgca gttaactttt
    11881 aatctcaccc tgaccaaaga ggagattcag cgatggctaa aagaggcaca atcgtgatcg
    11941 accccggcca cggcggcacc gtcgaagtcg gcggttcatc ccacaacaac gcgcgcagcc
    12001 cgagcggcgt cctcgaaaaa aatctgacgc tgcggatggc cttccacgtg cgcgacgcgc
    12061 tcgcagaggc cgccaccatc ggaggccaca cgatcaagat tctgttgacg cgcgagacgg
    12121 acaaaaacgt cggcctccgc gaccgcgccc tcttcgcaaa gaacaacaac gcagacctct
    12181 ttctctgcat tcacttcaac gcctccgtcg cgcacaacgc gcgcggcgtc gaaactctca
    12241 tcagcccgaa ggcgggcaac tcgaaccaca cggcagacaa ggcgttcgca cagttgatcc
    12301 agaccgccac cttcaacgcc atcaaggcgc acgacgcggg gacgaaagac cggaaggtta
    12361 aggatcaatc gctcgccgtc ctgcgcgagt cgagcctcgg cacgaaggcg cgcggctgtc
    12421 tcgtggagat ggagtttctc gatacgaagg cggttgacga actgttcaac ctcggcgcga
    12481 atgccccgaa ggtgcgcagc gacgtggcac agggcatcgc taacgcgctt atcgcagcct
    12541 tgtaaatccc tccctctgcg cctctctgcg cccgcgcgtg agatttatac cggatagaga
    12601 ataaactcag gccggtgcgc caaggcgcag cggaaggcgg ggacaggttt tttggcgagg
    12661 tgcgctcaaa ttattcgaca ggcggcggcg agttgggcta acatcgcacg taaatctgaa
    12721 gcgcgcgcaa gggcgcggga atcgtagtga tgttcgtttt ttacctgcca accagactcc
    12781 ttcttacgtt gccgctcggc gaccgcgcct gaacatgcgc gcgggtcggt tcgacgcggc
    12841 gaaacttcac caaagcgacc aattaaaatg tatgagccgg aggcggaagc tgtatcgagg
    12901 cttctcgcgc atccggcgtg tgatggtaag gtaagaagga agagtgtgac ttatggacga
    12961 aaaatatttt cccgaaaaga ttgaagagaa gtggcagcgc gtgtgggcgg agacgggcgc
    13021 gtttgaagtc gagcctgacg acgcccgacc gaagtttttt tgtctggaga tgctgcctta
    13081 cccgtccggc tttttgcaca tggggcacgt gcgcaactat tccatcggcg acgcgctggc
    13141 gtggcataaa cgcctgcaag gcttcaacgt gctgcacccg attggctggg acagcttcgg
    13201 gcagcccgcc gaacaggccg ccatcaaacg cggcgtccag ccgcgcgagt ggacggaaga
    13261 gaacatcgcg cacatgcgcg ggcagttcaa acgcctgggc atcagcttcg actggcggcg
    13321 cgagatggcc gcgcaccgtc ccgattatta ccgttgggac cagtggttct tcttgcagat
    13381 gctggcgcgc gggctggtct accggcgtac ttccgcgatc aactggtgcc cgaaggaaga
    13441 gactgtgttg tcgaacgagc aatcttcggg cggcgtctgc tggcgttgcg gaacgcccgt
    13501 ggagaaacgc gatctggaac agtggttcat ccgcacgacc gaatatgctg aagccttgct
    13561 cgccgacatg aaagagatcg aggcgggctg gccggagcgc gtgctgacga tgcagcgcaa
    13621 ctggatcggg cagagcatcg gcgcatacat agatttcccc atcaaggaca cggaggcgaa
    13681 agtgcgcgtc ttcaccacgc gcatcgacac catctacggc gcgacggcca tcgtcgtcgc
    13741 cacagagcac ccgctgctga acgaactgga agagtcgcct ttaaaagata aggtgatggc
    13801 cttcgtcgaa cgggtgaagg cagagcgcgc aagtcgcgcc gccatgccgg gcgaagaggt
    13861 ggagaaagag ggcgtcaata cggggctgtt cgccgtcaat cctttcagcg gcgagcagat
    13921 tcccgtctgg gtggcaaact acgtgttgat cgaatacggc gcgggcgcgg tgatggccgt
    13981 cccggcgcac gacgaacgcg attacgagtt tgcgcagaag tatagcctgc ccgtccgccg
    14041 cgtgatcgaa cgcgtctcgc acgagcagga gtccatcccg cacaacgaag cgtcggggct
    14101 ggatcaatcc accgcgatgc cgcacgcctt tacggactac ggcatcctcg tcaactccgc
    14161 cgagtggggc gggcgactgt cgaaggacgc gctgcgcgag atggccgcgc acgcggaggc
    14221 gaaaggtttc ggcggcggcg cggtcacgta ccgtctgcgc gattggggca tctcgcgcca
    14281 acgcttctgg ggcgcaccca tccccatcat ctactgcgac gcgtgcggca tcgtgcccgt
    14341 gccggaaacg gatttgcccg tcgttttgcc ggaacgcgcg gagtttacgg gcgcgggcaa
    14401 ttcgccgctg gcaaacgtca aggagttcgt caacacgact tgcccgaagt gcgggcaggc
    14461 ggcgcggcgc gagacggaca cgatggacac cttcgtcgat tcgtcgtggt acttcttccg
    14521 ctactgcgat ccgaagaaca cggacgcgcc ctttgataaa gcgcgcgtcg cctactggac
    14581 tccggttgat caatacatcg gcggcatcga gcatgccgtg atgcacctgc tctacacgcg
    14641 tttctggacg aagatgatgc gcgacatcgg actcgtcacg ttcgacgagc cggtgcggaa
    14701 tctgttgacg cagggcatgg tcacaaacgt cgtggaaggc acgggcgaat ggaaggcgat
    14761 gtcgaagtcg ctgggcaacg gcgtcgaccc ggacgagatg atcgaggctt acggcgcaga
    14821 cgccgtgcgc ctgttcgtca tgttcgccgc gccgccggag aacgaactgc gctggctgga
    14881 gacgggcatc gagggcgcgg tacgtttcct gcgccgcgtc tacaacttcg tctggcgttg
    14941 gcgcgcgagc ttgacggacg cgctatcgaa ggaggccgca ggcgcgttag agttcaacga
    15001 cgcgtcgcgc gctctgcgac gcaagacgca ccagaccatc gcgcgcatca cgcaggactt
    15061 ctcggagttg catttcaaca cgagcatcgc ggcattgatg gaactgttga acgcgctggg
    15121 cgagttcgag gtcaagccgg aggcggcgac ggaggcggac aaattcgccg tgcgcgaggc
    15181 gttggaggcg ttggtatcca tgctcgcgcc gtttacgccg cacgtcgcgg aggagttgtg
    15241 ggaggctctg ggacacgcgg gcggcctgct ggggagcggc gcgcgctggc ctgaggccga
    15301 tgcggaactg gcgcgcaaag aggctctgga aattcccgtc cagatcaacg gcaaactgcg
    15361 cgggcgcatc cacgccacgc ccgacacgtc cgaagaggaa ctgcgcacgg ctgcgctggc
    15421 cgaggaaaaa gtgcgcgccc tgatcgaagg gcgtcaaatc gtcaaagtga tcgtcgtccc
    15481 gcagcggctc gtcaacatcg tcgtgcgata gatgctgccg gtcgcccgtc gcctcttgtc
    15541 cgtcatccgt cgtcgccggt tgtttcacga ctcatgcgtg agtcgctaaa gattcaaccc
    15601 ggcagacggc gacgggcggc gggcgactga caaatctttc tccatgcttt agtctatgaa
    15661 gtatcgagac gatgattctc ataaccatga aggaaagttt ttcgcatgac ctctgagcaa
    15721 ccggcgagcg agccgaataa gaaactcctg acgggcgcgc gtgccgcgct cgccgcgctg
    15781 acgttctcgc tcttggcaat cgcactctct tcaagctgca accccacgga cagcacgcac
    15841 aacggcagcc ggaacgaccc gccgcccgcc gcgactccgg ctgcgggcag caaccccgcc
    15901 accgcgccgc cggggacagg tgcgccgccc acgcaacccg cgcggtcaac ttcgcccgcc
    15961 ccgttgccgg aggcgatagc gaacgccgcg atgaccacgc tcgacggcaa gccgctgcgc
    16021 ctgtccgacc tcaaggggaa ggtcgtcatc ctgaacctgt gggcgacgtg gtgcgggccg
    16081 tgtcgccggg agattcccga tttcatcaag atacaggaag actacaacgg gcgcggcgtg
    16141 gaagtgctcg gcataacttc ggaggacgaa cggaacacgg cggagatggt gaaggagttc
    16201 gtcaaggagt ttgagatcaa ctacaaggtg gtgtgggttg atcaggagac gtggggcgag
    16261 tttctcgcgc cgcgctatag catcccgcaa acgctggtgc tcgatcagaa cgggcaactg
    16321 ctgcaaaagt ttgtcggcta cagcccgcag gtggcggtga tggcgcgcga gatcgcagac
    16381 cgcgccctga gcggggcgac ggagagcggc ggtcgcacag accagacggc aaaataactc
    16441 gcgcgctcta cccgccgcga aacccgcccg cgtctcgaaa cgagaacact tcggcgagcg
    16501 actcgaagta gtggcgataa gatcagaggc agccggacat gcgtgtttgc acatgtccgg
    16561 ctgccttgtt ctttccggcg cgagtttccg taaagctccg gcggagcggg aaatcttctc
    16621 gcttattcgc cgccgctgcc ggagtcgctg ttctggcgtt cctgttccaa ctcgcgttcg
    16681 ccttcctcct ggagttcgcg cccttcgcgt tcgagttcct tatcggccaa gccctgcgcg
    16741 acgctctctt tcgcgcgccc ttccgcgatc ttcatctccg cctgcacgcg cccttccact
    16801 tcatctatcg cggcgggttc gcccgtctcc tcctgcgtct gcaccttatc cggatcaata
    16861 gacatctctt caaccacctc ctcagaaagc acgactcgcg tgaaaaattg atttcgggca
    16921 tcctctcact cttcgctccg cgcggtcaaa tggccgctcc cgaattgccg gaaaaatatc
    16981 ccgaaaaaat attttgccgg gg
//
LOCUS       DASWNS010000564         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_73182, whole genome shotgun sequence.
ACCESSION   DASWNS010000564 DASWNS010000000
VERSION     DASWNS010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73182"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VGB76_19575"
     CDS             <1..929
                     /locus_tag="VGB76_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101135.1"
                     /translation="LAFARRVEAGGTKRDLYLKIVRRTVLIYACGFLLAGFPFFNLST
                     IRLSGVLQRIAVCYFCAAVISLKTNPRTQSLIVAVLLLVYWGVSAFVAAPGFAAGDLS
                     KEGSLASFVDRVVLGEHIWKGGGRIYDPEGLLSTIPAIATTLTGLLAGHWLRARRADY
                     EKVSGLFVAGVACVVAGWMWNAWFPINKALWTSSYVLFTTGMALQLLACCYWLTDIKR
                     YRRWAVPFVIFGTNALAVFVLSGLAARLMNLWKMPRMDGRPGNLQTYIYEHLFATWLA
                     PADGSLLYALLYVLIWLGLMAILYQRRIFIKV"
     gene            complement(926..>1712)
                     /locus_tag="VGB76_19580"
     CDS             complement(926..>1712)
                     /locus_tag="VGB76_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101136.1"
                     /translation="YEGVRPRVGLRVQLKAIARNVLLAYQFMFYRFFPGAIVACLFIL
                     FYMSRRSLREIVRDVSAYWFLLLPPLVASGFYLLVHFEQRYFAPFVVVIGMSLFASLR
                     VPRSPEASRLVAALVFVTLAMFALSVGYYSARELYSTVRDLAPGRTAARADVQWQVAD
                     ELSRMGVGRGESVASIGNTMFNTWPRLARVRVVAEISTRTGGDVEKFWAADAALKAKV
                     IETFARTGAKVIVAEGIPQWSSQPDGWQKIGTTNYYVYFLPRP"
BASE COUNT          316 a          535 c          540 g          321 t
ORIGIN      
        1 cgctcgcctt cgcacgccgg gtcgaagcgg gcggaacgaa gcgcgatctc tatttgaaga
       61 tcgtgcggcg caccgtgctc atctacgcgt gcggcttttt gctcgcgggt tttccgttct
      121 tcaacctgtc caccatccgg ctctccggcg tgctgcaacg aatcgccgtc tgctacttct
      181 gcgccgccgt tatctctctc aagacgaacc cgcgcacgca aagtctgatc gtcgccgtgc
      241 tgctgctggt ttactggggc gtgtcggcgt ttgtcgccgc gccgggattt gccgcggggg
      301 atttgagcaa ggaaggcagc ctcgcttcgt tcgttgaccg cgtggtgctc ggcgagcaca
      361 tctggaaagg cggcgggcga atttacgacc ccgaaggact cctgagcacg atccctgcaa
      421 tcgcgacgac gctcaccggc ttgctcgcag gccactggct cagggcgcgg cgcgcggatt
      481 acgaaaaagt gtcggggctg tttgtcgcgg gggtggcgtg cgtcgtcgcg gggtggatgt
      541 ggaacgcctg gttcccgatc aacaaggctc tgtggacgag ttcttacgtc ctcttcacga
      601 cgggcatggc gttgcaactg ctcgcctgct gctactggct cacggatatc aagcgataca
      661 ggcgttgggc tgtgccgttc gttatcttcg gcacgaacgc gctcgcggtc ttcgtcttgt
      721 cgggactcgc ggcgcgcctg atgaatctct ggaagatgcc gcgcatggac ggcaggccgg
      781 gcaacttgca gacctacatc tacgaacacc tcttcgccac atggctcgcg cccgccgacg
      841 gctcgcttct ctacgcgctc ctctacgtgc tcatctggct cggcctgatg gcgattctct
      901 accagcgcag gatttttatt aaggtctagg gacgcgggag aaagtagacg taatagttcg
      961 tcgtcccgat cttctgccag ccgtccggct gcgacgacca ctgcgggatt ccttcggcca
     1021 cgatcacctt cgcacccgtg cgcgcgaaag tctctatgac ttttgctttg agcgcggcat
     1081 cggcagccca gaacttctcc acgtcgccgc ccgtgcgcgt cgaaatctcg gccaccacgc
     1141 gcacgcgcgc cagacgcggc cacgtgttga acatcgtatt gccgatagac gccacgctct
     1201 cgccgcgccc gacgcccatc cggctcaact cgtcggccac ctgccattgc acgtccgcgc
     1261 gcgccgcagt gcgccccggc gcgagatcgc gcaccgtcga ataaagttcc cgcgcgctgt
     1321 agtagccgac ggagagcgcg aacatggcga gcgtgacgaa gacgagcgcg gcgacgagac
     1381 gcgacgcctc cggcgagcgc ggcacacgca gagaagcgaa gaggctcatg ccgatgacga
     1441 cgacgaaggg ggcaaagtat ctctgctcga aatggacgag caggtagaag ccggaagcga
     1501 cgagcggagg caggagtaga aaccagtaag ccgacacgtc gcgcacgatc tcgcggagac
     1561 tgcggcggct catgtaaaaa agaataaaga gacaggcgac gattgcgccg gggaagaacc
     1621 tgtagaacat gaactggtaa gcaaggagga cgtttcgcgc gatggctttc agttgcacgc
     1681 gaaggccgac gcgcgggcgc acgccttcat ac
//
LOCUS       DASWNS010000565        12624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_331896, whole genome shotgun sequence.
ACCESSION   DASWNS010000565 DASWNS010000000
VERSION     DASWNS010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12624
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331896"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VGB76_19585"
     CDS             <1..395
                     /locus_tag="VGB76_19585"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0101137.1"
                     /translation="AKVEGVSLDGARLIPVAQDEASNSYSMLEPRMKKVYGAYYKELY
                     YTPERRILDPKVQELISIAASLVAKCDGCIDGHMKKALSLGATKEEISETICIAAAVN
                     AAAMIDLSDRTAARLGLNHFPNKPPEKV"
     gene            455..1468
                     /locus_tag="VGB76_19590"
     CDS             455..1468
                     /locus_tag="VGB76_19590"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0101138.1"
                     /translation="MKSRRAKNSEAAAASLHQSQAGGASCPLFLLTLASLLLSSCAAV
                     AQEAKAPAASAAEKRAAVGSSAFAGRVEYAEFASASLGRNVRYAVSLPPSYDRKSAQR
                     YPLVIFLHGLNNDERDWESHCIETKLEALRAASKVGDFIVAIPYGANSFYLNAQDGTR
                     YEDAIVKDFLPFIDKTYRTIASPRSRLIAGLSMGGYGALLIAFKHPELFAGVAAHSAA
                     LFEELPQAPKSPEDRGGKYRYDLATKLFGSPPDAAFFQANNPLYLARTNAAALKRLKI
                     YFDIGQSDRYGFEKGNLKLSETLKAAGVAHEYTLAPGAHGWSFLVDRSEPAFLFIWNT
                     LRT"
     gene            1648..2685
                     /locus_tag="VGB76_19595"
     CDS             1648..2685
                     /locus_tag="VGB76_19595"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid dehydrogenase"
                     /protein_id="HEY0101139.1"
                     /translation="MEIINHKSGTDHERVVFGREEASGYRGIIAIHSTRLGAALGGAR
                     FWNYASEADALVDVLRLARGMTYKNALAGLPFGGGKSIIIGDNRTTEREELFRAHGRF
                     VETLGGSYLTAEDVGTTTADMEFVSRETRHVAGLAHRSGDPSPVTARGVFRAMQAAAR
                     HRWGTDELAGRRVALQGCGHVGYFLAKELHEAGVELIVTDVERERVERVSRECLAESV
                     RPEEIYAVEADIFAPCALGGILNDETISQLRVEIVAGAANNQLLDDRHGESLAARGIL
                     YAPDYAANAGGVINGCIELLDWEQSRARAKVEAIYDTLLEIFQRAETDGIPTYKAADQ
                     LAEARLEHAHA"
     gene            3022..4704
                     /locus_tag="VGB76_19600"
     CDS             3022..4704
                     /locus_tag="VGB76_19600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HEY0101140.1"
                     /translation="MRSKKLSALVFSLLFSCALALLVSLPPSGAAHDGDKHIGEGGNG
                     QPDGQPMSAMSVTPCVNGMAGTFPCRNVDLLSFLPLASIGGGTGNDVWGWTDPLTNRE
                     YALMGRSNGTSFVDITDAEHPVYLGNLPTHSVDSVWRAIKVYGNHAFIVSEAANHGMQ
                     VFDLTRLRNVAAPPVTFTETAHYNGFSRAHTITINEETGFAYANGVTSYADPAANKCA
                     RGLHVINLHKPTQPTFAGCFDADGYTHDSQCVVYRGADATYRGREVCFNSNEDSLTIV
                     DVTDKNAPRQISRTTYPGSAYTHQGWLTDDQSLFLVDDELDEQKLGTKTRTHIFNVSD
                     LDAPSITGFHEGTSTAIDHNLYILGRYAYQSNYRSGLRVLDISHASTATLQEVGYFDV
                     YPVDDNPAFNGTWSNYPFFKSGVVIVSGIEQGLFMLRPTTNLVPTPQLLTEWNSTRAV
                     ALDSVTRTREPFSVNSSVNFSADGRTRLVLFAYNVDLAPGEPQTVLTAQAEDVQHRIY
                     PLAVEFVGKVPDFERVTQVVVVLPLELANAGDVSVSLNLRGTPGNKALIRIQ"
     gene            complement(4867..6345)
                     /locus_tag="VGB76_19605"
     CDS             complement(4867..6345)
                     /locus_tag="VGB76_19605"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0101141.1"
                     /translation="MNYAKLKFKRGETRSLYSQLVEALESAIEREDLTAGARLPSERA
                     LAVQLKLSRTTVVNAYRELEARGLLRSHVGRGTFVCARPEPADAPFAWRGKVSAFAQL
                     NADPALRNLHLDALNSRLISFAAGIPAIECFPLADYRRLLETVLKTQSDAVFGLGATE
                     GQMALRKAIAARTGTRPERVLILSGSQQGINLIARCLLDVGDTVVMDRPGYVGAIQTF
                     RAAGAKLIGWDTLRGDIDELEDLFLRYRPKFLYTNPTFQNPTGKVFTLRERRDLLRLA
                     VRYRIPIIEDDPYRETYLDAQPPPPTLYELDEHNIVIHHSTFSKVLAPGLRLGWLAAS
                     EYVVDQLALIKQRENLFTEGLSQLVVAEFLRSGLFDEHLIRLRREHAHRRDRMAAAIE
                     RHLPARLLTYARPKGGMSFWCRLGHGMRARQLLQGAMAAGVVFANGEIFYADEAGAHE
                     LRLCFASQPADKIEEGIKRLAASLKVKDERRAPREESLMPIV"
     gene            6563..7228
                     /locus_tag="VGB76_19610"
     CDS             6563..7228
                     /locus_tag="VGB76_19610"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0101142.1"
                     /translation="MDSFEATGRSALKRLPKRGLYERAGVYRILDEGFICHVGFVVEG
                     QPFVIPTGYARAGDTLYIHGAQASRMLRALKGGLDVCVTVTLVDGMVMARSAFHHSMN
                     YRSVVVFGRAVAVEESAEKLEALRALTEHLAPGRWDEVRQPTEGEMRATLVLALALEE
                     ASAKVRVGPPLDDEEDYALPVWAGVVPLRVVAGEPVPDPRLPQGIEPPAHVLNYARGA
                     GSA"
     gene            7267..7743
                     /locus_tag="VGB76_19615"
     CDS             7267..7743
                     /locus_tag="VGB76_19615"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0101143.1"
                     /translation="MIREWRRGEYAISTDRSRLDVGLVHRYLSESSYWAQGRPREVVE
                     RSIEHSLAFGVYLGERQVGFARVITDYATFAWIADVFVLEEFRGQGLGKWLSEVMIAH
                     PDLQGFRRWVLATKDAHELYRRVGFQELQRAERWMERRDPATVERPDYWATTTPGA"
     gene            7844..8782
                     /locus_tag="VGB76_19620"
     CDS             7844..8782
                     /locus_tag="VGB76_19620"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0101144.1"
                     /translation="MSAPVIVWLILSLIWGSTWLFIKLGLQDLPPFTFAGVRFVIAAA
                     ILLVVVAVRRLPLPRERGDWGLIALTGIMAFSVNYGLLFWGEQRTSSGLAAILQTIIP
                     AFGLVIAHYYLPDERITLWKVCGIVLGIAGVALIFSDQASVEGSSALAGSAAIVIGAF
                     CVAYSNVLVKARARHIEPAVLAAGQMICGLVPLLLVGVTFEGNPFSFRWTPLAIASLL
                     YLALVGSAIAFLLFYWLVRNMDVTKTMLIPLVTPPIAVFLGWLVLHEGLTWRTAAGAL
                     AIMSGIALIVTRKKKELQATPTAATATVAVKECETG"
     gene            8806..10017
                     /locus_tag="VGB76_19625"
     CDS             8806..10017
                     /locus_tag="VGB76_19625"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0101145.1"
                     /translation="MKDDALKDEEGDGREGLQTKAVHGGRAEHTTRAVSSPIWQTSTF
                     RADSSGHFAEIAAGTRPAEFYTRYGNPTHREAEATIAALEGGEAVLLAASGMGAIFTA
                     MLCNLRAGDHVVAQRNLYAGTNTLIEELLPRWGIEHTFVEQTDAANFAEVLRPSTKLI
                     YVETPTNPLMRITDLRAVAALGRERRITTMVDNTFATPINQRPLEFGIDVCVHSATKY
                     LGGHSDITAGVVVGSQEFVERAWKFGLVAGAVLSPFDGWLLLRGLRTLGLRVERHNAN
                     ALALARFLEAHPNVERVYYPGLESHPQHELARAQMSGYTGMLGVELKGDFRAAEKFIA
                     SLKLAMNAASLGGYETLVVHPAAMWGQQLSASQRAAMGVNDRLVRVSVGLEDEADLIR
                     DFAQALERALS"
     gene            10028..10960
                     /locus_tag="VGB76_19630"
     CDS             10028..10960
                     /locus_tag="VGB76_19630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY0101146.1"
                     /translation="MRKLHYHLVDVFTNRQFGGNPLAVFTNGRGVRVETMQAIAKELN
                     LSETTFVLPPTDGAANDYRVRIFTPGQELPMAGHPTVGTTYVLAREHMIERGEDGAET
                     EIKLEEGVGTIPVRIEWREGAANFIQMRQPLPTFGARVEDVRAVAEMLSIAPEEIAET
                     NLPLEVVSCGVPFLYVPVKSLRAVRNIKFRVDVWENKLKDTVPPEVFVFTREVEFPNS
                     TVHSRMFAPGMGIWEDPATGGASGPLGCYLVRYGVVSADEGGAAEFTSEQGIEMGRPS
                     FIKIRITREGEAITDVRVGGECRFMGEGYLEIED"
     gene            11055..12155
                     /locus_tag="VGB76_19635"
     CDS             11055..12155
                     /locus_tag="VGB76_19635"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0101147.1"
                     /translation="MSKVVVALIPEPHAPVELREVTEPELEQNSALLEVELSEVCGTD
                     VHLQQGRLAGVPYPLIPGHVSVGRLGKIRGRLLDVEGQPFAEGDRVTFLDVHRTCNAC
                     WHCLVARATTRCPARKVYGITYGLADGLSGGWAQKLYLKPNTRCIRLGETKMETFMAG
                     GCALPTALHAVERADVQLGDTVLVLGSGPVGLSAVIFAQMRGALRVLCIGAPAARLET
                     ARTVGASATLDIETNDEQARMSWVREQTNGRGADVCIEATGAPIAVAEAMRYTRDAGR
                     VCVVGQYTDHGEISFNPHLDLNRKHLDVRGCWGSDFSHFYRGAQIMADETRARPWSQM
                     KLERYSLARANDALSDVASTRVVKALIDPRLS"
     gene            12199..>12624
                     /locus_tag="VGB76_19640"
     CDS             12199..>12624
                     /locus_tag="VGB76_19640"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0101148.1"
                     /translation="MNGHEKSPESLAPAYVRWPYIGGAVTALALTSDSLVRDGLRKSF
                     HDDAKVTGERVEAYYRPLQTRGGQRAARLVRDQRDYTQIENSLDKIRQPALIVWGAED
                     QLSRLEDGKRLHSKLAGSQLVVFDNCGHLPQEEMPERFTR"
BASE COUNT         2414 a         3845 c         4010 g         2355 t
ORIGIN      
        1 cggcgaaggt cgaaggcgtc tcgctcgacg gcgcgcgttt gattccggtc gcacaggatg
       61 aagcctcgaa ctcttactcg atgcttgagc cgcgcatgaa gaaagtttac ggcgcgtact
      121 acaaagaact ctattacacg ccggagcggc gcatcctcga cccgaaagtg caggaactga
      181 tctcgattgc cgcttcgctc gtggcgaagt gcgacggctg catcgacggg cacatgaaga
      241 aggcgctctc gctcggcgcg acgaaggaag agatcagcga gacgatctgc atcgccgccg
      301 ccgtcaatgc cgccgccatg atcgacctca gcgaccgaac ggcggctcgg ctcggcctca
      361 accacttccc caacaagcct cccgaaaagg tttgaaggcg ggagcttgat ccgctgaacg
      421 gctgttgagt ttaatggctg ttgagttcga acgtatgaaa tctcgtcgtg cgaaaaatag
      481 cgaagcggct gcggcgtcgc tgcaccagtc gcaggcgggc ggcgcgtctt gccctctgtt
      541 tctcttgacg ctcgcgtcgc tgctgttgtc gtcctgcgcc gccgtcgcgc aggaagcgaa
      601 agctccggcg gcgagcgcgg cggagaagcg cgccgccgtc gggagcagcg cgtttgcggg
      661 gcgcgtcgag tatgcggagt ttgcgtctgc gagtctgggt cgcaacgtgc gctacgccgt
      721 gtcgcttccg ccttcgtatg acaggaagag tgcgcagcgt tacccgctcg tcatcttcct
      781 gcacggcctg aacaacgacg aacgcgactg ggagagccat tgcatcgaga cgaaactgga
      841 agccctgcgc gcggcctcca aggtcggcga ctttatcgtg gcgattcctt acggcgcgaa
      901 cagtttttac ctcaacgcgc aggacggcac acgctacgaa gacgccatcg tcaaagattt
      961 cctgcccttt attgataaga cttatcgcac catcgcttcg ccccgctcgc gtctcatcgc
     1021 ggggctttcg atgggcggct acggcgcgct cctcatcgcc ttcaaacatc ccgaactctt
     1081 cgcgggcgtt gccgcacaca gcgcggcact cttcgaagaa ttgccgcaag ccccgaagtc
     1141 gccggaagat cgcggaggga agtatcgcta cgatctcgcc accaaactct tcggctcgcc
     1201 gcctgatgcc gccttctttc aagccaacaa cccgctctat ctggcgcgca cgaacgccgc
     1261 cgcgttgaag cgtctgaaga tctatttcga catcggccag agcgaccgct acggcttcga
     1321 gaagggcaat ctcaaactct ccgagacgct caaggccgcc ggggtcgcac acgaatacac
     1381 gctcgcgccc ggcgcgcacg gttggtcgtt tctcgtagac cggagcgaac ccgccttcct
     1441 cttcatctgg aatacgctgc gcacataagc gtcgccgtcc gggttaaata tcttctcaag
     1501 tgccaatcat aaattggcta tttggtcttc gaacgccgaa tcataagatg acgcgattgc
     1561 agtgctgcgc cggtggataa cttgcgcgtc gaagcgtcgg cgtctttaaa ctctgcgccg
     1621 cgcgtgcgtg ctctctgagt ctgaaatatg gagatcatca atcacaagag cgggacggat
     1681 cacgagcgcg tcgtgtttgg ccgcgaagag gcgagcggct accgtggcat catcgcgatt
     1741 cacagcacgc gcctcggagc cgcgctcggc ggcgcacgct tctggaacta cgcgagcgaa
     1801 gcggacgcgc tcgtggacgt gctccggctc gcgcgcggca tgacctacaa gaatgcgctc
     1861 gcggggcttc ctttcggcgg cggcaaatcg atcattatcg gcgacaaccg gacgacggag
     1921 cgcgaagagc ttttccgcgc gcacggtcgc ttcgtagaga cgctcggcgg cagttattta
     1981 acggcggaag acgtggggac gacgacggct gacatggagt ttgtgtcgcg ggagacgcgg
     2041 cacgtcgccg gtctggcgca tcgttccggc gacccttcgc ccgtcacggc gcgcggcgtc
     2101 tttcgcgcca tgcaggccgc cgcgcgccac cgttggggaa cggacgaact cgcgggtcgc
     2161 cgtgtcgcct tacaaggctg cggccacgtc ggctactttc tggcgaaaga actgcacgaa
     2221 gcgggagtgg agttgatcgt cacggacgtt gaacgcgagc gggtcgagcg cgtgtcgcgc
     2281 gaatgccttg cggagagcgt caggccggaa gagatttacg cggtcgaggc ggacatcttt
     2341 gcgccctgcg cgctcggcgg catcctcaac gacgaaacga tttcgcaact cagagtggag
     2401 atcgtggccg gagcggcgaa caaccaactg ctcgacgacc ggcacggcga atcgctcgcg
     2461 gcgcgcggca tcctctacgc gcccgactac gcggcgaacg ccgggggcgt catcaacggc
     2521 tgcatcgaac tgctggattg ggaacagtcg cgcgcgcggg caaaagtcga agcgatctac
     2581 gacacgctgc tggagatttt ccagcgcgcc gaaaccgacg gcatcccgac ctacaaggcc
     2641 gccgaccaac tcgccgaagc gcgccttgaa cacgcgcacg cctaagccgt tcgcgcatgc
     2701 gtcgcgcaga gataaaattc cagagtttaa cggcgcacct ctccagccac tctccgcatc
     2761 tttcctttgt ctacggacga acgcgcgccg gcacgcgaca aaaaccgccg agtcgggcta
     2821 tgatgtgcgc atcaggaagc acgacggcgc agagcaggcc gtctgacggg agcgcgcagg
     2881 ccgcgccgca cccgcttccg cccgcagaga agtagtgcct ctggataacg ccttgaagtt
     2941 agtgccccgg aaaaatcttt cccgctaata tcaggcgtgc gttgatgaag taagtccgtc
     3001 tcttaaccag aagtaaaaaa aatgagaagt aaaaagttat ccgcgttggt cttctcgctg
     3061 ctgttttcgt gcgcgcttgc cctgctcgtg tcgctaccgc cttcgggcgc ggcgcatgac
     3121 ggcgacaaac atatcggtga gggtggaaac gggcaaccgg acgggcagcc gatgtctgcg
     3181 atgagcgtga cgccgtgcgt gaatggcatg gcgggcacgt tcccctgtcg aaacgtagac
     3241 ctcttatcgt tcctcccgct cgccagtatc ggcggcggca cgggcaacga tgtctggggc
     3301 tggactgatc cgctcaccaa cagagaatac gcgctcatgg gtcgttccaa cggaacttcc
     3361 ttcgtagaca tcacggacgc ggagcatccc gtctacctcg gcaacctgcc cacgcatagc
     3421 gtggattcgg tgtggcgcgc gatcaaagtc tacggcaatc acgccttcat cgtgagcgaa
     3481 gccgccaatc atggcatgca ggtctttgac ctcacacggc tgcgcaacgt cgccgcgccg
     3541 cccgtcacat ttaccgagac ggcgcactac aacggtttca gccgcgcgca tacgatcact
     3601 atcaacgaag agacgggctt tgcttacgcc aacggcgtga cctcctacgc agaccccgcc
     3661 gccaacaagt gcgcgcgcgg cctgcacgtc atcaacctgc ataagccgac ccagccgacc
     3721 ttcgccggat gcttcgacgc agacggttac acgcacgact cgcagtgcgt cgtttatcgc
     3781 ggcgccgatg cgacttatcg cggcagggaa gtctgcttca attccaacga agacagcctc
     3841 accatcgtgg atgtcaccga taagaacgcc ccgcgtcaaa tatcgcgcac cacctatccc
     3901 ggcagcgcgt acacgcatca gggttggttg accgacgacc aatcactgtt tctcgtagac
     3961 gacgaactcg acgagcaaaa gctcggcaca aagacccgca ctcacatctt caacgtctcc
     4021 gacctcgacg cgccttccat cacgggcttc cacgaaggca cgagcaccgc gattgatcac
     4081 aacctgtaca tcctcggtcg ttacgcttat caatccaact accggagcgg cctgcgcgtg
     4141 ctcgacatca gccacgccag tacggccact ctgcaagagg tcggctattt cgatgtctac
     4201 ccggttgacg acaatccggc cttcaacggc acttggagta attacccgtt cttcaagagc
     4261 ggcgtcgtca tcgtgagcgg catcgagcag ggactcttca tgctccgacc gacgacgaat
     4321 cttgtcccga cgccccaact tctcaccgaa tggaactcga cccgcgccgt cgcgctcgac
     4381 tcggtgacgc gcacgcgcga gcctttctcc gtcaactcaa gcgtgaactt cagcgcggac
     4441 gggcgcacgc gcctcgtgct ctttgcctac aacgtggatc tcgcgcccgg cgagcctcag
     4501 acggtgctga cggcgcaggc cgaagacgtt caacatcgaa tctatcctct cgcagtggag
     4561 ttcgtcggca aggttcccga tttcgaacgg gtgacgcaag tggtcgtcgt cctgcctctc
     4621 gaactggcaa acgcgggcga tgtctccgtc agcctcaacc tgcgtggcac gcccggcaac
     4681 aaggcgttga ttagaattca gtgatgagtg acaagtaaca agttaaaaag cggtgaatcg
     4741 taaatcgtag atcgtgaata gcagaaagca tttcttctct attcacgatc tacgattcac
     4801 cgctttttct catcactcat cactcatcac tcatcacttt tctttctcgt ccctcgtcac
     4861 tgctatttaa actatcggca tcaaactctc ttcgcgcggc gcgcggcgtt cgtccttaac
     4921 cttgagactc gcggcgagcc tctttattcc ttcctcgatc ttgtccgccg gttggctcgc
     4981 gaagcagaga cgcagttcgt gcgcccccgc ttcgtccgca tagaaaatct cgccgttggc
     5041 aaagacgacg cccgccgcca ttgctccctg aagcagttgt cgcgcgcgca tgccgtgccc
     5101 caacctgcac cagaaagaca tgccgccttt cgggcgcgcg taggtcaaga ggcgtgcggg
     5161 cagatgtcgc tcgatggctg cggccatgcg gtcgcgccgg tgcgcgtgct cccgccgcaa
     5221 cctgatgagg tgttcgtcga acaggccgct ccgcaaaaac tcagccacca ccaactggct
     5281 caacccttcc gtaaacaggt tctcgcgctg cttgatgagc gcaagttgat ctacgacgta
     5341 ttcggacgcg gcgagccagc cgagacgcaa gcccggcgcg agcactttgg agaaggtgct
     5401 gtgatgaatg acgatgttgt gctcgtcgag ttcgtagagc gtgggcggcg ggggctgcgc
     5461 atcgagataa gtttcgcgat aaggatcgtc ctcgatgatg gggatgcgat agcgcacggc
     5521 gaggcggagc aggtcgcgtc gctcgcggag cgtgaagact ttgcccgtcg ggttctggaa
     5581 ggtcgggttg gtgtagagaa acttcgggcg atagcgtagg aacagatctt cgagttcgtc
     5641 tatgtcgccg cgcagcgtgt cccagccgat gagttttgcg cccgccgcgc gaaacgtctg
     5701 gatcgcgccg acgtagccgg ggcggtccat caccaccgtg tcgcccacgt cgagcaggca
     5761 acgcgcaatg agattgatgc cctgttgcga gccggagagg atgagcacgc gttcggggcg
     5821 cgtccccgtg cgtgcggcga tggccttgcg cagtgccatc tgcccctcgg ttgcacccag
     5881 accgaacacc gcatcgctct gggttttcaa caccgtctcc aacagccgcc ggtagtcggc
     5941 gagcggaaaa cattcgatgg cgggaatccc tgcggcgaaa gagatgagac gcgagttgag
     6001 cgcgtcgagg tgcaaattac gcagagccgg atcggcgttg agttgggcga aggcggaaac
     6061 tttgccgcgc cacgcaaacg gcgcgtccgc cggttcggga cgcgcgcaga cgaaagtgcc
     6121 gcgcccgacg tggctgcgca agaggccgcg cgcttcgagt tcgcggtaag cgttgacaac
     6181 cgtggtgcgg ctcaacttga gttggacggc cagcgcgcgt tcggagggca ggcgcgcgcc
     6241 cgccgtcaag tcttcgcgct cgatggcaga ctccaaagcc tcgacaagtt gactgtagag
     6301 cgaacgcgtc tcgccgcgct taaacttcag cttcgcgtag ttcatcactt ctcaagcata
     6361 gaccgcaact tccaggctgc tcaagtgcca attgcaaatt ggctatttat gatctatgcg
     6421 acagccgtta gtatggcgga aatagacggg ttaagaggtg aatcgcaggg aggccaatgc
     6481 agggagatta aaggccaatt tgcgatttga acgcgggtac gacgctcgtg caatgggaga
     6541 atcacgtcgg agaggtgaga gaatggatag ttttgaggcg acgggaagaa gcgcgttgaa
     6601 gcgtctgccg aagcggggct tgtacgagcg cgcgggcgtc tatcgaattt tagatgaggg
     6661 tttcatctgt catgtcggct tcgtcgtcga ggggcaaccg ttcgtcatcc cgacgggcta
     6721 tgcgcgcgcc ggggacacgc tctacattca cggtgcgcag gccagccgga tgctccgcgc
     6781 gctcaagggt ggcctcgacg tgtgcgtgac ggtgacgctc gtggacggca tggtgatggc
     6841 gcgctcggcc tttcatcatt cgatgaacta caggtcggtc gtggtcttcg gtcgcgccgt
     6901 cgcggtagaa gagagcgcgg agaaactcga agcgttgcgc gccttgacgg aacaccttgc
     6961 gccgggtcgt tgggatgagg tgcggcaacc gacggaaggc gagatgcgtg cgacgctggt
     7021 gctcgcgctc gcactcgaag aagcgtccgc gaaggtgcgc gtgggaccac ctctggacga
     7081 tgaagaggat tacgcgctgc cggtgtgggc aggcgtcgtg ccgctccggg tggtggcggg
     7141 cgagccggtt cccgatccgc gtctgccgca aggcatcgag cctccggcgc acgtgttgaa
     7201 ttacgcgaga ggcgcaggga gcgcgtgagc gcgggcgtct gtttgccggg aggggagaga
     7261 agagagatga ttcgtgaatg gcggcggggc gaatatgcga tcagcacgga tcgctcgcgt
     7321 ttggatgtcg ggttggtgca caggtatttg agcgagtcgt cttactgggc gcagggtcgt
     7381 ccgcgcgaag tcgtggaacg ctccatcgaa cattcgctgg cgttcggcgt ctatctgggc
     7441 gagcggcagg tcgggttcgc gcgtgtgatc acggactacg ccacgtttgc gtggatcgcg
     7501 gacgtgttcg tcctggaaga atttcgcggg cagggcttgg gcaagtggct cagcgaagtg
     7561 atgatcgcgc acccggactt gcaggggttt cgccgctggg tgctggcgac gaaagacgcg
     7621 cacgagcttt atcgccgcgt cggctttcaa gagttgcagc gcgccgaacg ctggatggaa
     7681 cggcgcgacc cggccacggt ggagcggcct gactactggg cgacgacgac gccgggcgca
     7741 tgagattact caagctctca agacgcgaag atgcgaaggc cgacccgccg gcttctttgc
     7801 gccggagtgt cacggtgtta acttcgagca ggcaggatat tcaatgagcg cacccgttat
     7861 cgtctggctg atactgagtt tgatctgggg ttcgacgtgg ctcttcatca aactaggctt
     7921 acaggatttg ccgcccttca catttgcggg cgtgcggttc gtgatcgccg ccgcgatcct
     7981 gctcgtcgtc gtcgccgtcc gcagactgcc gctgccgcgc gagcggggcg attgggggct
     8041 tatcgcgctc acgggcatca tggctttctc cgtcaactac gggttgctct tctggggcga
     8101 gcaacgcacc tcttcgggcc tcgcggcgat cctgcaaacg atcatcccgg ccttcggcct
     8161 tgtcatcgca cactactatc tgccggacga acgcatcacg ctctggaagg tgtgcggcat
     8221 cgtgctgggg atcgcgggcg tggccttgat cttttcggat caggcgagcg tggaagggag
     8281 ttcggcgttg gcggggagcg cggccatcgt gatcggcgcg ttctgcgtgg cctattcgaa
     8341 cgtgctggtg aaagcgcgtg cgcgccatat cgaaccggcg gtgctggcgg cgggacagat
     8401 gatctgcgga ctcgtgccgc tcctgctcgt cggcgtgacg tttgaaggaa accccttctc
     8461 atttcgctgg acgccgcttg ccatcgcgtc gctcctgtat ctcgcgctcg tcggctcggc
     8521 catcgccttt ttgttgttct actggctcgt gcgcaacatg gacgtgacga agacaatgct
     8581 catcccgctc gttacgccgc ccatcgcggt cttcctcgga tggctcgtcc tgcacgaagg
     8641 gctgacgtgg cgcacggctg cgggcgcgct cgccatcatg tccggtatcg ctttgatcgt
     8701 gacgcgcaag aagaaagagt tgcaagcgac accgacggcg gcgacggcga ccgtcgcggt
     8761 caaggagtgc gagacgggtt gagttctatt gaaagggagc aagcgatgaa agatgacgcg
     8821 ttgaaagatg aagagggcga cgggcgcgaa gggttgcaga cgaaggccgt acacgggggg
     8881 cgcgcggagc atacgacgcg tgcggtgagt tcgccgatct ggcagacctc aacttttcgc
     8941 gccgattcct ccgggcactt tgcagagatc gccgccggta cacgccccgc cgaattctac
     9001 acgcgctacg gcaacccgac gcacagggag gccgaagcca ccatcgccgc actcgaaggg
     9061 ggcgaggccg tgctccttgc ggcctcgggc atgggcgcga tcttcactgc gatgctctgc
     9121 aacctgcgcg cgggcgatca tgtcgtggcg cagcgcaacc tctacgcggg cacaaacacg
     9181 ctgatcgaag agttgctgcc gcgctggggc atcgagcaca cgttcgtcga gcagaccgac
     9241 gccgcaaact tcgcggaagt cctgcgcccg agcacgaaac tcatctacgt ggagacgccg
     9301 accaacccgc tcatgcggat cactgacctg cgcgccgtcg ccgcgctcgg tcgcgagcga
     9361 cgaatcacga cgatggttga caacacgttt gccacgccca tcaatcaacg ccctttggag
     9421 ttcggcatag acgtgtgcgt gcacagcgcg acgaaatatc tgggcggcca cagcgacatc
     9481 acggcgggcg tcgtcgtcgg ctcgcaggag ttcgtcgaac gcgcctggaa gttcgggctg
     9541 gtcgcgggcg cggtcttgag tccgttcgac ggctggctgc tcctgcgtgg cttgcgcacg
     9601 ctcggcctga gagtagagcg gcacaacgcg aacgcgctcg cgctcgcgcg tttcctcgaa
     9661 gcgcacccga acgtggagcg cgtctattat cccggcctcg aatcgcaccc gcagcacgaa
     9721 ctcgcgcgcg cgcagatgag cggctacacg ggcatgctcg gcgttgaact caaaggcgac
     9781 tttcgcgcgg ctgagaagtt catcgcttcg cttaaactgg cgatgaacgc ggcgagcctc
     9841 ggcggctacg aaacgctcgt cgtgcatccg gcggccatgt gggggcaaca actttcagcc
     9901 tcacagcgcg cggcgatggg cgtgaacgac aggctcgtgc gtgtctcggt cgggctggaa
     9961 gacgaagcgg acttgattag ggatttcgcg caggcgttgg agcgcgcgct tagttgagag
    10021 acacggcatg agaaaacttc attatcatct ggttgatgtc ttcaccaacc ggcaattcgg
    10081 cggaaaccct ctggcggttt ttacgaacgg gcgcggcgtc cgcgtggaga cgatgcaggc
    10141 gattgccaaa gaattgaatt tatctgagac gaccttcgtg ctgccgccca ccgatggcgc
    10201 ggcgaacgat taccgcgtcc gcatcttcac gccgggacag gaactaccga tggccgggca
    10261 tccgaccgtc gggacgactt acgtgctcgc gcgcgaacac atgatcgaac gcggcgagga
    10321 tggcgcggag acggagatca aactcgaaga gggcgtcggc acgatccccg tccgcatcga
    10381 gtggcgagag ggcgcggcga acttcataca gatgcggcag cccttgccga ccttcggcgc
    10441 gcgcgtcgaa gatgtgcggg cggtcgcgga gatgttatca atcgcgcccg aagagatcgc
    10501 ggagacaaac ctgccgctcg aagtcgtctc ctgtggcgtg ccgtttctct acgtgccggt
    10561 gaagagcttg cgcgcggtac ggaacatcaa gtttcgcgta gacgtgtggg agaacaagct
    10621 gaaagatacg gttccgccgg aagtcttcgt cttcacgcgt gaagttgagt ttccgaattc
    10681 gaccgtgcac agccgcatgt ttgcgcccgg catgggcatc tgggaagacc ctgcgacggg
    10741 cggcgcgagt ggaccgctcg gctgttatct cgtgcgctac ggcgtcgtct cggctgacga
    10801 gggcggcgcg gcggagttta cgagcgagca gggcatcgag atgggacgtc cgagtttcat
    10861 caagatcagg atcacgcgcg aaggtgaagc gatcacggac gtgcgcgtcg gcggcgagtg
    10921 tcgcttcatg ggcgaaggtt atcttgagat tgaagattag ttaagccgcc acaggatgta
    10981 aatcatggcg ttgctcaagc agaccgcccg caaacgagtc tgacggcacg gcctaaacct
    11041 ctgagttgaa tgatatgagc aaagtggtcg tcgcattgat ccccgaaccg cacgcgcccg
    11101 tcgagttgcg cgaggtgacg gagccggagt tggagcagaa ctccgcgctg ctcgaagtgg
    11161 aactctccga ggtctgcggc acggacgtgc atctgcaaca ggggcgactc gcgggcgtgc
    11221 cctacccgct catccccgga cacgtctcgg tcggtcggct cggcaaaatt cgtggccgtt
    11281 tgttagacgt tgaagggcag cctttcgccg aaggcgaccg cgtgaccttt ctcgacgtgc
    11341 atcgaacctg caacgcctgc tggcattgcc tcgtcgcacg cgccaccacg cgctgccccg
    11401 cgcgcaaagt ctacggcatc acctacggcc tcgctgacgg gctatccggc ggctgggcgc
    11461 agaagcttta cctcaaaccg aacacgcgct gcattcgttt gggcgagacg aagatggaga
    11521 cgttcatggc gggcgggtgc gcgctgccca cggctctcca cgccgtcgaa cgcgcggacg
    11581 tgcaactcgg cgacaccgtg ctcgtcctcg gcagtggtcc cgtgggactc tccgccgtca
    11641 tcttcgcgca gatgcgcggc gcgctccgcg tgctctgcat cggcgcaccc gccgcgcggc
    11701 ttgagacggc gcgcaccgtc ggcgcaagcg cgacgctcga catcgagacg aacgatgaac
    11761 aggcgcgcat gagttgggtg cgggaacaga cgaacgggcg cggcgcggac gtgtgtatcg
    11821 aagcgacggg cgcgcccatc gcggtggcag aggccatgcg ctacacgcgc gacgcggggc
    11881 gcgtgtgcgt cgtcggtcag tacacggatc acggcgaaat ttcattcaac ccgcatctcg
    11941 acctcaaccg caagcatctc gacgtgcgcg gctgctgggg atcggacttc tcccattttt
    12001 atcgcggcgc gcagatcatg gcggacgaga cgcgtgcgcg cccctggtcg cagatgaaac
    12061 tcgaacgcta cagcctcgcg cgtgccaacg acgcgctctc ggatgtcgct tcaacgcgcg
    12121 tcgtcaaggc gttgatcgat ccgcgcctgt cttaacgcgc cgcatacgcg gatgagccgc
    12181 ttggctctcc gttgaaattt gaacggccat gaaaaaagtc ctgagtcgct cgctcccgcc
    12241 tacgtgcgct ggccttacat cggcggggcg gtcaccgcgc tcgcgctcac ctccgactcg
    12301 ctggtgcgcg acggcctgcg caaaagtttt cacgacgatg cgaaagtgac tggcgaacgc
    12361 gtagaggctt actatcgccc gctccaaacg cgcggcgggc aacgcgcggc gcggctcgtg
    12421 cgcgaccagc gcgactacac gcagatcgaa aactcgctcg ataaaattcg ccaacccgcg
    12481 ctcatcgtct ggggcgcaga agatcaactg agccggttgg aagacggcaa gcgtctacac
    12541 tcgaagctcg caggctcgca gctagtcgtc ttcgataatt gcgggcatct cccgcaggaa
    12601 gagatgccgg agcggttcac gcgc
//
LOCUS       DASWNS010000566         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_331961, whole genome shotgun sequence.
ACCESSION   DASWNS010000566 DASWNS010000000
VERSION     DASWNS010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331961"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /gene="gcvPA"
                     /locus_tag="VGB76_19645"
     CDS             <1..1029
                     /gene="gcvPA"
                     /locus_tag="VGB76_19645"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEY0101149.1"
                     /translation="LQAIFEFQTLVCQLTGMDVANASMYDGSTSVAEAVLMAERITRR
                     RKVVVSGALHPEYLHVAETYVAHAGIDLVRAGFDEATGTTNFDDAHALDEQTAAIIVQ
                     SPNFFGCVEDLQSLSERAHKVGALLVVCVTESISLGLLREPGAAGADIVVAEGQSFGV
                     PLSYGGPYLGLFATREKYARQLPGRLVGVAADKEGRRGFVLTLATREQHIRREKATSN
                     ICTNEGLIALMATIYIETLGRQGLQEVAMQCAQKAAYAARRIGELEGFSLPYAATPRF
                     NEFVVRAPVAAEELLRRLAAEHDITGGLALSRYFPERPNDILVCVTETNPRAEIDALV
                     AGLAALTK"
     gene            1118..2647
                     /gene="gcvPB"
                     /locus_tag="VGB76_19650"
     CDS             1118..2647
                     /gene="gcvPB"
                     /locus_tag="VGB76_19650"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEY0101150.1"
                     /translation="MNTPKATTHITQNESLIFERSQTGRKGYRLPALDVEETPLDELL
                     PAAFRRTDDLEGVPEVSEVDVVRHFTRISTWNYNIDLGMYPLGSCTMKYNSRLNEKVA
                     RIRGFANLHPLAAEEDSQGALQLLYELQQHLSEITGLAAVSLQPAAGAHGEMTGVMII
                     RAFLDARDGVGEGDRPRRHVMLIPDSAHGTNPASAHLSGFTVKTVRSTPEGQTDLEAL
                     RELCAAGDVAGLMLTNPNTVGLFERSIKEICQIVHDAGGLVYMDGANMNALVGWARPG
                     DMGVDVIHLNLHKTFSTPHGGGGPGSGPCCCTKELEPYLPVPRIVRAGEDRYKLDFNY
                     PKTIGRVKAFYGNFGMAVRALSYIETHGADGLREATEAAVLNARVIAHGLAADYDKPF
                     DAPPMHEVVFTDKRQARKGVHTLDIAKRLIDYGFHPMTIYFPLIVQGAMLIEPTESVG
                     RQEIQQFIEAMRAIAREALENPELVTNAPHTTRIGRLDEAAAARKPVLRWRPEEKAAV
                     A"
     gene            2682..2969
                     /locus_tag="VGB76_19655"
     CDS             2682..2969
                     /locus_tag="VGB76_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101151.1"
                     /translation="MNFHSSRVALIKGISPLPLADEDSPSKQLAYGFNPAALRMSKRL
                     TRSGLSSTSDTSSSMDASSLRMKRVRRAVVEGRPLLYFELVSQQAAGSEEI"
BASE COUNT          606 a         1006 c          843 g          529 t
ORIGIN      
        1 ctgcaagcta tcttcgagtt tcaaacactc gtctgccagt tgaccggcat ggacgtggcg
       61 aacgcctcga tgtacgacgg ctcaacctct gtggccgagg ccgtgctgat ggccgaacgc
      121 atcacgcgcc gccgcaaagt ggtcgtctcc ggcgcgctcc atcccgaata tctgcacgtc
      181 gccgaaactt acgtcgcgca cgccggaatc gatctcgtcc gcgcgggctt cgacgaagcg
      241 accggcacga cgaacttcga cgacgcgcac gcgctcgacg aacagacggc ggccatcatc
      301 gtccaatcgc cgaacttttt cggctgcgtc gaagatttac agtcgctctc cgaacgcgcg
      361 cataaggtcg gcgcgctcct cgtcgtctgc gtcaccgaaa gcatctcgct cggccttttg
      421 cgtgaaccgg gcgcggcggg cgcggacatc gtcgtcgccg aaggccaatc gttcggcgtg
      481 cctttgagct acggcggccc ctatctcggc ctcttcgcca cgcgcgaaaa gtatgcgcgg
      541 cagttgcccg gcaggctcgt cggcgtcgcg gctgacaaag aaggcaggcg cggcttcgtc
      601 ctgacactcg ccacgcgcga acagcacatc cgccgcgaga aggccacttc aaacatctgc
      661 accaacgaag gtttgatcgc gctgatggcg acgatctaca tcgagacgct cggacggcag
      721 gggcttcagg aagtcgccat gcagtgcgcg caaaaggccg cctacgccgc acgccgcatc
      781 ggcgaactcg aaggcttctc gctgccctac gcagccacgc cgcgcttcaa cgaatttgtc
      841 gtgcgcgcgc ccgtcgccgc cgaagaactg ctgcgtcggc tcgccgccga acacgacatc
      901 acgggcggcc tcgccttgtc gcgctacttc cccgaacgcc cgaacgacat tctcgtctgc
      961 gtcaccgaaa ccaacccgcg cgcagaaata gacgcgctcg tcgcaggact cgcagccctg
     1021 acgaaatgag atttcaagtt acagctttca agtttcagtg acgtggtttc ggttctaaaa
     1081 ctcgtcacct gtcactcgtc acttgtcact ttttattatg aacactccga aagccactac
     1141 gcacatcacg cagaatgagt ctttgatctt tgaacgctcg cagacggggc gcaaaggcta
     1201 caggctgccc gcgctcgacg ttgaagagac gccgttggac gaactgttgc ccgccgcgtt
     1261 tcgtcgcaca gacgatctgg agggtgtgcc cgaagtctcc gaggttgacg tggtgcgcca
     1321 tttcacgcgc atctcgacgt ggaactacaa cattgatcta ggcatgtacc cgctcggctc
     1381 ctgcacgatg aagtacaact cgcgtctgaa tgaaaaagtc gcgcgtatac gcggcttcgc
     1441 caacctgcac ccgctcgccg ccgaagaaga ttcgcagggc gcactccaac tgctctacga
     1501 actccaacag catctctcgg agatcaccgg cctcgctgcc gtctccctgc aaccggcggc
     1561 aggcgcgcac ggcgagatga ccggcgtgat gatcattcgc gccttcctcg atgcgcgcga
     1621 cggcgtaggc gaaggcgacc gcccgcgccg ccacgtcatg ctcatccccg attcggcgca
     1681 cggcacgaac ccggcctccg cgcatctctc cggcttcacc gtcaagaccg tgcgctcgac
     1741 gcccgaagga cagactgacc tcgaagccct gcgcgagtta tgcgcggcgg gcgacgtggc
     1801 cggattgatg ttgaccaacc cgaacacggt cggcctcttc gagcgcagca tcaaagagat
     1861 ttgccagatc gtccacgacg cgggtggcct tgtttacatg gacggcgcga acatgaacgc
     1921 gctcgtcggc tgggcacgcc ccggcgacat gggcgtggac gtgatccacc tcaacctgca
     1981 caagaccttc tccaccccgc acggaggcgg cggtcccggc tccggaccct gctgctgcac
     2041 gaaagaactc gaaccatatc tgcccgtccc gcgcatcgtc cgcgccggag aggatcgcta
     2101 caagttagac ttcaactacc cgaaaacaat cggtcgcgtc aaagccttct acggcaactt
     2161 cggcatggcc gtgcgcgcgc tctcctacat cgagacacac ggcgcggacg gtttgcgcga
     2221 agcgaccgaa gccgccgtcc tgaacgcacg cgtcatcgcg cacgggttag ccgccgacta
     2281 cgataagccg ttcgatgcgc cgccgatgca tgaagtcgtc ttcacagaca aacgacaggc
     2341 gcgcaaaggc gtccacacgc tggacatcgc caagcgtctc atcgactacg gcttccaccc
     2401 gatgaccatc tacttcccgc ttatcgtgca gggcgcaatg ctcatcgagc cgaccgaatc
     2461 cgtcgggcga caggagatac aacagttcat agaggccatg cgtgccatcg cccgcgaagc
     2521 gttggagaat cccgaactcg tcacgaacgc gccacacacg acgcgcatcg gacgcctcga
     2581 cgaagccgcc gccgcgcgca aacccgtcct gcgctggcga ccggaagaga aagccgccgt
     2641 cgcttaaaca aacattcctc caaacgaact aaacgcgacg cgtgaacttt catagttcgc
     2701 gcgtcgcgtt gattaagggc atcagcccgc tacctttggc cgatgaagac tcgccctcga
     2761 aacaactcgc ttacggcttc aaccctgccg cattgaggat gtcgaaacgg ctcacgcgct
     2821 ccggtctgtc gagcacgtcc gacacttcct cttcgatgga cgcgtcatct ctcaggatga
     2881 agcgtgtgag gcgtgcggta gttgaagggc gaccgcttct ctatttcgag ttggtgtcgc
     2941 agcaggccgc cgggtcggaa gagatttgag ttgaagcgca ttag
//
LOCUS       DASWNS010000567         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_306496, whole genome shotgun sequence.
ACCESSION   DASWNS010000567 DASWNS010000000
VERSION     DASWNS010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306496"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1073
                     /locus_tag="VGB76_19660"
     CDS             102..1073
                     /locus_tag="VGB76_19660"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0101152.1"
                     /translation="MRKVLLTGATGFIGRHCLRALVAAGGSFDEVHAVSTRGDACDSA
                     REPAVDFNSAATKASVVRWHRANLLSNEDVQRVLAEVQPSHLLHLAWYAEPGKYWTSP
                     VNEEWLRASERLLREFVERGGERVVVAGTCAEYDWNDGGYCSERATRLAPATLYGKCK
                     DALRNALEELARDTGLSAAWGRIFFLYGTHEHPQRLVASVIRSLLREEVARCTHGEQL
                     RDFLYVQDVADAFVALLESDVRGAVNIASGEVVNLKTVIERIAEKLDRPHLIRLGALP
                     APAGEPKLLAADVSRLRDEVRWSPRYTLDAGLEATINWWRAQPDSVA"
     gene            1115..1999
                     /locus_tag="VGB76_19665"
     CDS             1115..1999
                     /locus_tag="VGB76_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101153.1"
                     /translation="MTKICVVHLVWIPLGLAQFAEFIASYREHEAGVEHDLLIIFKEL
                     EQEDQLAAYEELLAGINYKTMFLGSEGFDLVPYFTAAKKFDYQYFCFLNSYSVILAAN
                     WLRLMLTHLQTRGVGLVGATGSYESYYSNLLQSRPRFGVRSLPGDVRRYRHWRQMLVE
                     AREHFEPFPNEHLRSNAFMIARELMLQINFRGEADKMESLKFESGKEGLTRQVRALDL
                     QALVVGRDGAAYAGAEWPHSHTFRSGGQRNLLVADNRTRQYAEADAPTQKFLEQCAWG
                     SLRSESTAARNSLRETRF"
     gene            1983..3017
                     /locus_tag="VGB76_19670"
     CDS             1983..3017
                     /locus_tag="VGB76_19670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0101154.1"
                     /translation="MKLDFNEAVKPSMNNRPLVSIGMPLYNAERYVREALDALLAQDY
                     PDFELIISDNASTDATASICREYAARDRRIQYQRAESNMGAVWNFRRVFELSRGQYFM
                     WAAFDDLRAPQYLSQCVAALEARPEAVMCCTDIRLIDQDGREVGETEFPRGIRMAGRK
                     VSERVRAISRSLYWYDFYGLMRRDVLATTRLPQPSWGFDVILMLEMSLRGEVLLVREK
                     LFAYRIIKGKTGEQAAATLGPAHARGALSINWGGLTAELFHSIWLAPLRTTEKLSLTF
                     QFLLGFCVRNPLVRIQLYREGLSGATGAWRRGRYGLSLGLLSIAAVVIPVEAVYSLCV
                     RPLRYRHAKT"
     gene            3082..4353
                     /locus_tag="VGB76_19675"
     CDS             3082..4353
                     /locus_tag="VGB76_19675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0101155.1"
                     /translation="MKIAVWHNLPSGGGKRALYDHVSGLLRRGHRVESWCPPTADQSY
                     LPLGELTTERVLPLDWQPRAEKRLPAKLATLRLDPLTRIEALDRHCRRCAEEINRGGF
                     DLLFANSSLYQAVSSIGRYVRLPKVIYLQEPTREFFEAMPEPLWNALPPAASHLSPSY
                     FRAYLKDLIKTQTWRAMLREEVRNAKAFDAVLVNSFYSRESILRAYGVDSKVCYLGID
                     AAKFVDLDLPRENYVVGLGAFVKLKNVEFALRSLAAMPAPRPRLVWIGNAANPAYLEE
                     MRQLAASLDVPFEPLVKIDDDELVKVLNRAMLMIYASRLEPFGLAPLEANACGTPVLA
                     VAEGGVRETIVDGVNGLLVDNDPRAMAAAITRLSQDREYARRLGQTARREVVERWSVE
                     ASVERLEQRLGEAVADHQAASDVQGRERSQH"
     gene            4567..>4700
                     /locus_tag="VGB76_19680"
     CDS             4567..>4700
                     /locus_tag="VGB76_19680"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0101156.1"
                     /translation="MTKKALITGITGQDGSYLAEHLLSLGYEVHGLVRRVALEDAARR
                     "
BASE COUNT          894 a         1418 c         1499 g          889 t
ORIGIN      
        1 cgctggaacg acccggcctt cggcgtccag tggccggaga cggcgggcgg cggggagcgc
       61 atcatcatcg cgcgcgaccg tgagtatccc gatttcactc tatgaggaaa gttctgctca
      121 ccggggcgac cggcttcatc ggtcggcatt gcctgcgcgc gctcgtcgcc gctggcggga
      181 gcttcgatga agttcacgcc gtctccacgc gcggagacgc atgtgactct gcgcgtgagc
      241 ctgccgttga cttcaactct gcggcgacga aggcgtccgt cgtgcgctgg cacagagcga
      301 acctgctctc gaatgaggac gtgcagcgcg tgctcgccga ggtgcagccg tcacacttgc
      361 tgcacctggc ctggtacgcg gagccgggga agtactggac ttcgcccgtg aacgaggaat
      421 ggctgcgcgc gagcgagcga ctgctgcggg agtttgtcga acgagggggc gagcgcgtcg
      481 tcgtcgccgg aacgtgcgcc gagtacgact ggaacgacgg cggctactgc tccgagcggg
      541 caacgcggct tgcgcccgcg accctctacg gaaaatgtaa agatgcgctc cgcaatgcgc
      601 tcgaagaact ggcgcgcgac acaggcttga gcgcggcgtg gggacgaatc tttttcctct
      661 acgggacgca cgagcacccg caacgcctcg tcgcctccgt cattcgctcg ctgctgcgcg
      721 aagaggtcgc gcgctgcacg cacggcgaac agttgcgtga ttttctctac gtgcaggatg
      781 tcgcagacgc cttcgtggcc ttgcttgaga gcgacgtgcg cggggcggtc aatatcgctt
      841 cgggcgaggt tgtaaatttg aagacggtca tcgaacggat cgcggagaaa ctggatcgcc
      901 cgcacctgat ccgactcggc gcgctccccg ccccggcggg cgagccgaaa cttctggccg
      961 cagacgtttc gcgcctgaga gatgaggtgc gctggtcgcc ccgctacacg ctcgacgctg
     1021 gtttggaggc gacgatcaac tggtggcgag cgcagccgga tagtgtcgct tgagcgtgga
     1081 gatgccctgt ggagcgttcg ggcaggtcgc tggcatgacg aaaatttgcg tcgtacattt
     1141 agtctggatt ccgctggggc tggcgcagtt cgccgagttc atcgcttcgt acagagagca
     1201 cgaggccggg gtcgaacacg acctcctgat aattttcaag gaactggaac aggaagatca
     1261 actcgccgct tatgaagagt tgctcgcggg gatcaactac aagaccatgt ttttagggag
     1321 cgaaggtttc gacctcgtgc cttactttac ggcggcgaaa aagtttgact atcaatactt
     1381 ttgcttcctc aattcttaca gcgtgattct cgcggccaac tggctgcggc tgatgttgac
     1441 gcacctgcaa acgcgcggcg tcggcttagt cggggcaacc ggctcttacg aaagttacta
     1501 ttcgaacctg ttgcaaagtc gcccgcgctt cggcgtcagg agtctgcccg gagacgtgag
     1561 aagatacagg cattggcgac agatgttggt cgaagcgcgg gagcacttcg agccgtttcc
     1621 gaacgaacac ctgcgcagca acgcctttat gatcgcgcgc gaactgatgc tccaaatcaa
     1681 tttcaggggt gaagccgata agatggagtc gctcaagttc gaatccggca aagaaggctt
     1741 gacgcggcag gtgcgcgcgc tggacttgca ggcgttggtg gtggggcgcg acggcgcggc
     1801 ttacgccgga gcagagtggc cgcacagcca cactttcagg agcggcgggc agcgcaattt
     1861 gctcgtcgca gataatcgca cgcggcaata cgcggaagcc gacgcgccga cccaaaagtt
     1921 tctggaacag tgtgcgtggg gaagcctgcg cagcgaatca accgccgcgc gcaactcgct
     1981 ccgtgaaact cgattttaat gaagccgtga agccttcgat gaataaccga ccgctcgtga
     2041 gcatcgggat gccgctctat aacgccgagc ggtacgtccg cgaagctctg gacgccctgc
     2101 tcgcgcagga ctatccagac ttcgagttga ttatttccga caacgcctcg acggacgcga
     2161 ccgcgagtat ctgccgcgag tatgccgcgc gcgaccggcg gattcagtat caacgtgcgg
     2221 agagcaacat gggcgcggtc tggaattttc gacgcgtctt cgagttgtcg cgcggccaat
     2281 attttatgtg ggcggccttc gacgacctgc gcgccccgca gtatctcagc cagtgcgtcg
     2341 cggctcttga agcgcgcccc gaagcggtga tgtgctgcac ggacattcgc ctcatcgatc
     2401 aggacgggcg cgaggtcggc gagacggagt ttccgcgcgg catcaggatg gccggacgca
     2461 aggtgagcga gcgcgtgcgc gccatctcgc gttcgctcta ctggtacgac ttctacggcc
     2521 tcatgcgccg cgacgtgctc gccacgaccc gcctgccgca gccgagttgg ggcttcgacg
     2581 tgatcctgat gctggagatg agtctgcgcg gcgaagtgct gctggtgcgg gaaaagctgt
     2641 tcgcttaccg catcatcaag ggcaagaccg gagagcaggc cgccgccact ctgggacccg
     2701 ctcatgcgcg cggcgcgctc tctatcaact ggggcggcct gaccgccgaa ctcttccata
     2761 gcatctggct cgcgcccctg cgcacgacgg aaaagttgtc gctcacgttc caatttctgt
     2821 tgggcttctg cgtgcgcaat ccgctggtca ggattcaact ctaccgtgaa ggattatccg
     2881 gcgcgaccgg agcgtggcga cgcgggcgtt atggcctctc gctcggactg ctctcgattg
     2941 cggcggtcgt cattccggtc gaagccgtct atagcctctg cgtgcgcccg ctccgttatc
     3001 gccacgcgaa aacctgaaag cgcgcgcacg gtgaagacga aaaaaggatt gagaagcgaa
     3061 gaacgcgcgg gacattcgag aatgaagatt gcagtctggc acaacctgcc gagcggcggc
     3121 ggcaaaaggg cgctctatga tcacgtcagc ggtctactga gacgcgggca cagggtggag
     3181 tcttggtgcc cgccgacggc ggatcaaagt tacctgccgc tcggcgagtt gacgaccgaa
     3241 cgcgtcttgc cgctcgactg gcagccgcgc gcggagaaga gactccccgc gaaactagcg
     3301 acgctgcgac tcgatccgct gacacgcatc gaagcgctcg accggcattg tcgccggtgt
     3361 gcggaggaga tcaaccgggg cggattcgat ctgctcttcg ccaacagttc gttgtatcag
     3421 gcggtcagtt ccatcgggcg atacgtccgc ctgccgaaag ttatctactt gcaggagccg
     3481 acgcgcgaat ttttcgaggc catgccggag ccgctctgga acgctctgcc gcccgccgcg
     3541 tcgcacctct cgccctccta ctttcgcgct tacctgaaag acttgatcaa gacgcagacg
     3601 tggcgcgcga tgctgcgtga agaggtgcgc aacgcgaaag ccttcgatgc cgtgctcgtc
     3661 aattcattct acagccgcga gagcatcctg cgcgcttacg gggtggactc gaaggtctgt
     3721 tacctgggca tcgacgccgc aaagttcgtt gacctcgatc tcccgcgcga gaactatgtg
     3781 gtggggttgg gcgcattcgt caaactcaag aacgtggagt ttgcgctgcg gtcgctggct
     3841 gcgatgcccg cgccgcgccc gcgtctcgtc tggatcggaa acgccgccaa ccccgcctac
     3901 ctcgaagaga tgagacaact cgccgcttcg ctcgacgttc ccttcgaacc gctcgtcaag
     3961 atcgacgacg acgaattggt gaaagttctg aatcgtgcca tgctgatgat ctacgcctcg
     4021 cgtctggaac ccttcggcct tgcgccgctc gaagcaaacg cctgcggcac gcccgtcttg
     4081 gcggtcgccg agggaggcgt gcgggaaacc atcgttgacg gagttaacgg cctgctggtg
     4141 gacaacgacc cgcgtgcgat ggcagcggcc atcacgcgcc tctcgcagga tcgggagtac
     4201 gcgcgccgcc tcggccagac ggcgcgaagg gaggtggtcg agcgttggtc ggtcgaagcc
     4261 tccgtcgagc gtctggaaca gagactcgga gaggccgtcg cagatcatca agcggcgtct
     4321 gacgttcagg gcagagagcg ttcgcagcac tgaaggcatc gagcgcgcct gcgacgcaaa
     4381 gaacattttt tgccggtcga acaaatagtc cggtcgcggc ctcggttacg gctgaggccg
     4441 cctttttgcc cgtgtttggc cgggcatcgc cgcacgcgca ccgattttcg gggaataagc
     4501 catttgattc tcccttcaaa gaactttatg ctttaccatc aacaacagaa atccggagta
     4561 taaccattga cgaagaaggc attaatcacc ggcatcaccg gacaggacgg cagctactta
     4621 gccgaacatc tcctctcgct cggctacgaa gtccacggac tcgtccgccg cgtcgccctc
     4681 gaagacgccg cccgccgctt
//
LOCUS       DASWNS010000568         7283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_175894, whole genome shotgun sequence.
ACCESSION   DASWNS010000568 DASWNS010000000
VERSION     DASWNS010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175894"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="VGB76_19685"
     CDS             complement(<1..1482)
                     /locus_tag="VGB76_19685"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY0101157.1"
                     /translation="MLKRKFAGLFLLCVLTFAPLAPLVRAQTTPFLSDEEIRMLSNEI
                     SGDRAFEHIRWLTHWHRDSGQEGYFKAAEYIVKAAKEAGLTDVRFIEQPLPGVNYTPR
                     SAELWMTEPVALKLADLGEHAVYLADGSHDADVTAELVYIGDATAESLKNVDVKGKIV
                     LTNWQPGRAVQNAVYASGAVGVVTYATTEGRSALDFPDQIAWTRINPTPPEGKKGTFA
                     FSLPPRKGETLRRILETNGMQDYFGTGKSTKGGRVVLRAKVDTEITPAPGRTGFVEGW
                     IRGSSIHDQQIILTAHLQEEQGSANDDGSGCGNLLELARTYNKLIAEGKMARPLRDIR
                     FWWTDEIYSEYRYFQDNPDEPKKFLANVHQDMTGANQAMGSRVQHLIFAPHSRTSYLD
                     AIFESVGNYVINTNNGFLAASRQGGLPRPHTRPLYATRGSRQGFNARFVPWFGSSDHM
                     TFLDGPINTPAVALINWDDNYIHSSDDDLFQIDQTQLRRNNFII"
     gene            complement(1591..5022)
                     /locus_tag="VGB76_19690"
     CDS             complement(1591..5022)
                     /locus_tag="VGB76_19690"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101158.1"
                     /translation="MRSNVTTNITSRAAFCLRLALLLFLISVAPPALHAQEEKQTERE
                     AVEKAKQEEAKKAETEKPKQEEAKKPEEAKRPADPLSAPTFAGLRLRLIGPAFTSGRI
                     VGIAVEPKDKNRYYVAAASGGVWKTINNGTTWTPVFDNEGSYSIGVVTLDPKNPLTVW
                     VGTGENNSQRSVSYGNGVYRSDDGGRNWKNVGLKTSEHIGRIVIDPRDSNVVYVAAQG
                     PLWGAGGERGLYKTTDNGATWKQILKISENTGVTDVVIDPNNPDTLYAAAYMRRRHVF
                     TMVNGGPESALYKSTDAGATWSKLTRGLPTSDMGRIGLAISPIDSNVLYATVEAADRK
                     GGIFRSTDRGGNWERRNEFDTTAMYYSQIYCDPKDLERIYVMNVFAMVSDDGGRTLRR
                     LGERSKHVDNHYLWIDPDNTNHYLIGSDGGVYESYDRAATWDFKENLPITQFYDVTTD
                     NAAPFYNVYGGTQDNFSVGGPSRTRSASGITNADWFVTQGGDGFRSQVDPQDPNIIYA
                     EYQHGGLARFDKRTGERVGIQPATGRGEEPLRLNWDVPFIISPHLHTRLYFAANRLYR
                     SDDRGDSWTVVSGELSRALDRDRLPVFGKVQGVDAVARHASTAFYGNASALAESQKKE
                     GLIYVGSDDGLVQVTEDGGRNWRKIEKFPGVPDMAYVSRIVTSNHDANTVYASFDNHW
                     NADFAPYLLKSTDAGRTWVSLRGDLPANGPVLAVAEDHVNPNLLFAGTEFGLFFSVNG
                     GGKWVQLKGNFPVIAVRDLAIQRRENDLVVGTFGRGIYVLDNYAPLRHVSAEMLRAES
                     FLFPVKDALMYIQSQPLGGRGKVFQGESFYTAENPPFGATFTYYLKETIKTRKVRRQE
                     SEKEAERRNAVVTLPTAEDLRAEEEEETPVIILTVTDATGRVVRRLTGAVTAGLQRVT
                     WDLRSPSPTLGAPPAPDAEADPFFEAPAGPLVMPGAYKVSIARRVGGVLTPLAAPQRF
                     NVVVEGQSSMSATDRAALLEFQQKVARLQRAVAGALETANALKPRLVSIRRALLDTPS
                     AGDKLLDDATAIDRSVNDILRSLRGDTVLRARNQNTPPSINDRVGQIIGEQRMAIARP
                     TGTHVAQYQIAAQEFEGVLARLRALIEGDLARLEKAMEAAGAPHTPGRIPEWKEN"
     gene            5192..5395
                     /locus_tag="VGB76_19695"
     CDS             5192..5395
                     /locus_tag="VGB76_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101159.1"
                     /translation="MASEKYHSPARRATGVFTAASGDGKRAWQTARGNARTERGRGSA
                     SEICLVTGISLRCAAAARLRFLP"
     gene            5534..6013
                     /locus_tag="VGB76_19700"
     CDS             5534..6013
                     /locus_tag="VGB76_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101160.1"
                     /translation="MEGLRNKASTLLRDYVTERRSTRRRGARYVARIPFNVSVVPDSQ
                     PGGATQAKPGASLAGHTRDMGEDDLNLIVPSIRIGSDYLTIKDNKLSVTLELPSGAVN
                     LIAVPARFEQLEPEGEGYLVGVRIEAMSAPERELYLQHLRTRPQADRRRDTLHLREA"
     gene            complement(6106..>7283)
                     /locus_tag="VGB76_19705"
     CDS             complement(6106..>7283)
                     /locus_tag="VGB76_19705"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0101161.1"
                     /translation="FRLVNAPVSDPQPKNWKEILPVRKGVTLEGLTMFANYYVVSERD
                     QGLNKMRIVDLKSGQHHYLEFPEPVYSANVNFTPEFDTSTLRFSYQSFTTPSSVYDYD
                     MKSRKRDLLKQQEVLGGYDATKYAAERVYAVAPDGVRVPVSLYYRKDLRKAGAQPLLL
                     SGYGSYGIPSNVGFSSGRLSLIDRGVIFAIAHIRGGGDLGKEWHDEGKMMKKKNTFID
                     FIAAAEHLTKEKYTAKDQLVITGGSAGGLLMGAVVNMRPDLFKAVVSYVPFVDVINTM
                     MDASLPLTVQEYLEWGNPNEKEAYAYMKSYSPYDNLAAKEYPAMLVKTSLNDSQVMYW
                     EPAKYVAKLRTLKTDKNPLLIKINMGAGHGGASGRYDALRETAFDYVFILQQFGITN"
BASE COUNT         1281 a         2144 c         2372 g         1486 t
ORIGIN      
        1 gatgatgaag ttgttgcggc gcagttgggt ttgatcgatc tggaagaggt cgtcgtcgga
       61 cgagtggatg tagttgtcgt cccagttgat gagggcgacg gcgggcgtgt tgatggggcc
      121 gtcgaggaaa gtcatgtgat cggaggaacc gaaccacggg acgaagcgcg cgttgaagcc
      181 ctgacgcgag ccgcgcgtgg cgtagagcgg gcgcgtgtgc gggcgcggga gtccgccctg
      241 acggctggcg gcgagaaagc cgttgttggt gttgatgacg tagttgccga ctgactcgaa
      301 gatcgcgtcg agataagagg tgcgcgaatg cggcgcgaag atgagatgct ggacgcgact
      361 gcccatcgcc tgattcgcgc cggtcatgtc ctgatggacg ttggcgagaa acttcttcgg
      421 ctcgtctgga ttatcttgaa agtaacgata ctcggaatag atttcgtccg tccaccagaa
      481 gcgtatgtcg cgcagggggc gtgccatttt gccctcggcg atcagtttgt tgtaggtgcg
      541 cgcgagttcg agaaggttgc cgcagccgct cccgtcgtcg ttggcagagc cttgctcttc
      601 ctgcaagtgc gcggtgagga tgatctgctg gtcgtgtatg gaactgccgc gtatccaacc
      661 ttcgacgaag ccggtgcgac cgggcgcagg cgtgatctcc gtgtcaactt tcgcgcgcag
      721 cacgacgcga ccgcccttcg tgcttttgcc cgtgccgaag tagtcctgca tcccgttggt
      781 ttcgaggatg cggcgcagcg tctcgccttt gcgcggcggg agcgagaagg cgaaagtccc
      841 ttttttgcct tcgggcggcg taggattgat gcgcgtccag gcgatctggt ccgggaagtc
      901 caacgcgctc cggccttcgg tcgtggcgta ggtgacgacg ccgaccgcgc cgctcgcata
      961 gactgcgttc tgcacagccc tgcccggttg ccagttcgtt aagacgatct ttcccttcac
     1021 gtctacgttc ttcaaactct ccgccgtcgc gtcgccgatg tagacgagtt cggcggtcac
     1081 gtctgcatcg tggctgccgt ctgcgagata gacggcgtgc tcgccgaggt ctgcgagttt
     1141 gagcgcgacg ggttcggtca tccagagttc cgcgctgcgc ggcgtgtaat tgacgccggg
     1201 cagcggttgc tcgatgaagc gcacgtccgt aagcccggcc tctttcgcgg ccttgacgat
     1261 atactctgcg gctttgaaat agccttcctg gccggaatcc cggtgccagt gggtgagcca
     1321 gcggatgtgc tcgaaggcgc ggtcgccgga gatttcgttc gaaagcatgc gaatctcttc
     1381 gtccgacaga aacggcgtcg tctgcgcacg cacgagcggc gcaagcggcg cgaacgtcag
     1441 gacgcacaac agaaacaggc cagcgaattt tcttttcaac ataaatctcc cgttcgaagg
     1501 gatgagggat gagggatgaa taagagaaag cactcatggc gttgagtggt ttctggttca
     1561 tccctcatcc ctcatccttc atccttgctt ttaattctct ttccattccg ggatgcgtcc
     1621 cggcgtgtgg ggtgcgcctg cggcctccat tgctttctca aggcgtgcga ggtcgccttc
     1681 gatgagcgcg cgcaggcgtg cgagcacgcc ctcgaactcc tgcgcggcga tctgatactg
     1741 cgcgacgtgc gttccggtcg ggcgcgcgat agccatgcgc tgctcgccga tgatctgccc
     1801 gacgcggtcg ttgatggaag gcggcgtgtt ttgattgcgc gcgcgcagca cggtgtcgcc
     1861 gcgaagcgag cgcaggatgt cgttgacgct gcgatcaatc gccgtggcgt cgtctaaaag
     1921 tttatcgccc gccgacggcg tgtcgagaag cgcgcgacga atggagacga gtcggggctt
     1981 gagcgcattc gccgtctcca gagcgcccgc gaccgcgcgt tgcaggcgcg ccaccttctg
     2041 ttgaaattca aggagcgcgg cgcgatccgt cgcagacatg ctcgactgcc cttcgaccac
     2101 gacgttgaac ctctgcggcg cggccagtgg cgtcagcaca ccacccacgc gcctggcgat
     2161 tgagactttg tacgcgccgg gcatgacgag gggtccggcg ggagcttcaa agaacgggtc
     2221 agcttccgcg tcgggcgcgg ggggcgcacc gagcgtcggc gaaggcgaac gcaagtccca
     2281 cgtcacgcgc tgtaggccag ccgtcacggc tcctgtgaga cggcgcacga cgcgtccggt
     2341 cgcgtccgtc acagtcagga tgatgacggg cgtttcttcc tcttcctccg cgcgcaggtc
     2401 ttcggcagtc gggagcgtga cgacggcgtt gcgtcgctcc gcttccttct ccgactcctg
     2461 acggcgcacc ttgcgcgtct tgatcgtctc tttcagatag taagtgaagg tcgcgccgaa
     2521 gggcggattc tccgccgtgt agaaagactc gccctgaaag accttgccgc gcccgcccag
     2581 aggttgcgac tggatgtaca tgagcgcgtc tttgacggga aacaagaagc tctcggcgcg
     2641 cagcatctcg gcggagacgt ggcgcagcgg cgcgtagttg tcgagcacgt agatgccgcg
     2701 cccgaacgtg ccgacgacga gatcgttttc gcgtcgctgg atggcgaggt cgcgcacggc
     2761 aatcacgggg aagttgcctt tgagttgaac ccacttcccg ccgccgttca cggagaagaa
     2821 gaggccgaac tccgtgcccg cgaacaacag gttcggattg acgtgatcct ctgcgacggc
     2881 gagcacggga ccattcgcgg gcaggtcgcc cctgagcgaa acccatgtgc gccccgcgtc
     2941 cgtggatttc aaaagatagg gcgcgaagtc tgcattccag tggttatcga aagaggcata
     3001 gacggtgttg gcatcgtggt tggaagtgac gatgcgcgag acataagcca tgtcgggcac
     3061 gccgggaaac ttctctatct tgcgccagtt gcgccctccg tcctccgtca cctgcacaag
     3121 gccgtcgtcg ctgccgacgt agatcaagcc ctctttcttt tgcgactcgg cgagcgcgga
     3181 ggcgttgccg taaaaggccg tcgaggcgtg gcgcgcgacc gcatccacgc cctgaacttt
     3241 tccgaacacg ggcagacggt cgcggtcgag tgcgcgcgag agttcgccgc tcaccaccgt
     3301 ccaactgtcg ccccggtcat cgctgcgata gagacgattg gcggcgaagt agagacgcgt
     3361 gtggaggtgc gggctgatga tgaagggcac gtcccagttc agacgcagag gctcttcgcc
     3421 gcgcccggtg gcgggctgga tgccgacgcg ttcgcccgtg cgtttgtcga agcgcgcgag
     3481 tccgccgtgc tgatattcgg cgtagatgat gttcgggtct tgggggtcaa cctgcgaacg
     3541 aaagccgtcg ccgccctgcg tcacgaacca gtcggcgttc gtgatgccgg acgcgctgcg
     3601 cgtgcgcgaa gggccgccga cgctgaagtt gtcctgcgtg ccgccgtaga cgttgtagaa
     3661 gggcgcggcg ttgtcggtcg tcacgtcgta gaactgcgtg atcggaaggt tctctttgaa
     3721 gtcccacgtc gcggcgcggt cgtaagattc gtagacgccg ccgtccgagc cgatgaggta
     3781 gtgattcgtg ttgtcgggat cgatccagag atagtgatta tcgacgtgct tcgagcgttc
     3841 gccgagacga cgcagcgttc tgccgccgtc gtccgaaacc atcgcaaaga cgttcatcac
     3901 gtagatgcgt tcgaggtctt tcgggtcgca atagatttgc gagtagtaca tcgcggtcgt
     3961 gtcaaactcg ttgcgccgct cccagttgcc gccccggtcc gtcgaacgaa agatgccgcc
     4021 ctttctgtcc gccgcttcga cggtggcgta gagcacgttc gaatcgatgg gcgagatggc
     4081 gaggccgatg cgccccatgt cggaagtcgg gaggccgcgc gtgagtttgc tccacgtcgc
     4141 gcccgcgtcc gtggatttgt agagcgcgct ctcaggacca ccgttgacca tcgtgaagac
     4201 gtggcggcgg cgcatgtaag cggcggcgta gagcgtgtcc gggttgttcg ggtcgatgac
     4261 gacatcggtg acgcccgtgt tctcgctgat cttcaaaatc tgcttccacg tcgcgccgtt
     4321 gtcggtggtt ttatagaggc cgcgttcgcc gcccgctccc cagagcggac cctgcgcggc
     4381 gacgtagacg acgttggagt cgcgcgggtc gatgacgatg cgcccgatgt gctcggaggt
     4441 tttgaggccg acgtttttcc aattgcgccc gccgtcgtcg gaacgataga cgccgttgcc
     4501 gtaggagacg ctgcgctggc tgttgttctc gcccgtgccg acccagacgg tgagcgggtt
     4561 cttcgggtcg agcgtgacga cgccgatgga atacgagcct tcgttgtcga acacgggcgt
     4621 ccacgtcgtc ccgttgttga tcgtcttcca cacgccgccc gacgcggcgg cgacgtagta
     4681 acggttcttg tccttcggtt cgacggcgat gccgacgatg cgcccggagg tgaaggcggg
     4741 accaatgagg cgcaggcgta agcccgcgaa ggtgggcgcg ctcaaagggt cggcgggacg
     4801 cttcgcctct tccggcttct tcgcctcttc ctgtttcggc ttctcggttt cggccttctt
     4861 cgcctcctcc tgtttcgctt tctcgacggc ctctcgctcc gtctgcttct cctcctgcgc
     4921 gtgcagcgcg ggcggggcga cggagatgag gaacaggagg agcgcgaggc gcaggcagaa
     4981 ggccgcgcgg gatgtgatgt tcgtcgtaac gttagagcgc atgcgggcgt tttctcctag
     5041 agtagacggg cgatgtcgtg tagtacgcaa cgggtggcgg ttagttgcag gaagcgtgcg
     5101 gaggccggac ggacgaatcg cacgttcgag cctctgctca ttccagcacg aataaaattc
     5161 tttcgggaga cgactgatta gagacggctg tatggcaagc gaaaaatatc attcccccgc
     5221 ccgccgtgca acgggagttt tcacggcggc gagcggcgac ggcaaacgcg cgtggcagac
     5281 agcgcgcggc aacgcccgca ccgaaagagg gcgcgggagc gcgtctgaaa tatgtctggt
     5341 gaccggaata agtttgcgct gcgccgcagc cgcccgcctg agatttttgc catgatctcg
     5401 acgggcgatg ttatgatgag gccacagtcc ggcggtcttc cccccaattt atgtttccct
     5461 gagccgcgtc gccgttcgaa atttgacggg cgcgaattaa cccgacacct tttgcagagg
     5521 agacatcacg agcatggaag gattaagaaa caaagcctcg acgctgttac gcgattatgt
     5581 cacggagcgg cgcagcacac gccgacgcgg cgcgcgctac gtcgcgcgca tcccgttcaa
     5641 cgtctccgtc gtgcccgact cacagccggg cggcgcgacg caagcaaagc ccggcgcgtc
     5701 tctcgccggt cacacgcgcg acatggggga ggacgacctg aacttgatcg tgccgtccat
     5761 acgcatcggc agcgattatc tcaccatcaa agataacaaa ctctcggtca cgctcgaact
     5821 tccgtccggc gcagtcaact tgatcgccgt gcccgcacgc ttcgagcaac tcgaaccgga
     5881 gggcgaaggc tatctcgtcg gcgtgcgcat cgaagccatg agcgcgcccg aacgcgaact
     5941 ctacctgcaa cacctgcgca cgcgcccgca agccgaccgg cgcagggaca cgctccacct
     6001 ccgcgaagcc tgagacaagg agaacgcgga agcacgaacg cggaacgatg aactgaagac
     6061 atttgctttc agttcatcgt tccgcgttcc tgcttctgcg tctctttaat tcgtgatgcc
     6121 gaactgctgc aagatgaaga cgtaatcgaa ggccgtttcg cgcagcgcgt cgtagcgacc
     6181 cgatgcgccg ccgtgtcccg cgcccatgtt gatcttgatg aggagcgggt tcttgtccgt
     6241 cttgagcgtg cgcagtttgg cgacatactt ggccggctcc cagtacatca cctggctgtc
     6301 gttcagagaa gtcttaacga gcatcgccgg gtactctttg gccgcgaggt tgtcgtaagg
     6361 actgtaagac ttcatgtaag cataagcctc tttctcgttc gggttgcccc attcgagata
     6421 ttcctgcacg gtgagcggca gcgaggcgtc catcatcgtg ttgatgacat cgacgaaagg
     6481 cacgtagctc acgaccgcct tgaagagatc gggacgcatg ttcacgaccg cgcccatcaa
     6541 caacccgccc gcgctgccgc ccgtgatgac gagttgatct ttcgccgtgt acttctcctt
     6601 ggtcagatgc tctgcggcgg cgatgaagtc tatgaaggtg ttcttcttct tcatcatctt
     6661 gccttcgtcg tgccactctt tgccgaggtc gccgccgccg cgtatgtgcg cgatggcgaa
     6721 gatcacgccc cggtcgatga gcgagaggcg accggaactg aaacccacgt tggaagggat
     6781 gccataagaa ccgtaaccgc tcaggagcag cggttgcgcg cccgccttgc gcaggtcttt
     6841 gcgatagtag agcgagacgg gcacgcgcac gccgtcaggc gcgacggcat agacgcgctc
     6901 cgccgcgtac ttcgtggcgt cgtagccgcc gagcacctcc tgctgtttga gcaggtcgcg
     6961 cttgcgcgat ttcatgtcgt agtcgtagac gctggagggc gtcgtgaacg attgataact
     7021 gaagcggagg gtgctcgtgt cgaactccgg cgtgaagttg acgtttgcgg aatagaccgg
     7081 ttcgggaaat tcgagatagt gatgctggcc gctcttgaga tcgacgatac gcatcttgtt
     7141 caaaccctga tcgcgctccg agacgacata gtaattggcg aacatcgtca acccttcgag
     7201 cgtgacgcct ttgcgcacgg gcagaatctc tttccagttc ttcggctgcg ggtcgctcac
     7261 gggcgcattg acgagacgga agc
//
LOCUS       DASWNS010000569         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_438017, whole genome shotgun sequence.
ACCESSION   DASWNS010000569 DASWNS010000000
VERSION     DASWNS010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438017"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="VGB76_19710"
     CDS             <1..1463
                     /locus_tag="VGB76_19710"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0101162.1"
                     /translation="LTQSADFFERHRTNYLVSRLVSSAAAIEAAVTGTLRDMLRESFT
                     LIAFLSASFYYSWRLTLGSLLIAPIIGVLTAKFGKNLRNLARETFEGSQRLVDTAQES
                     LANHHIVKAYRGEEREQRRFTTVARQIVSANLRSARINGFAPPSIEMIGVIAVAILLY
                     FGRREIMAGNMNPAQFLTFLFFLFSSYDPMRKLSRLQNALEIALAAARHVWEVMDENF
                     ETVERAEGAVELKPLRDSIELRDVSFDYANNEERPVLRSINLRLPAGKMVALVGESGG
                     GKSTLSKLILRFHDPTSGAILWDGTDLRDASLKSLRQHIGLVTQETVLFNDTVRYNIS
                     YSRPDATTAQIEEAARVAFAHDFIKDLPEGYDTIVGERGIFLSGGQRQRLAIARAVLA
                     DASVLVLDEATSALDAESERLVQQALSNLTRNRTTLVIAHRLSTIRRADSIVVMERGR
                     IIEQGKHEELLTQSGTYKKLYELQFADEEEEVVVSP"
     gene            1557..>1828
                     /locus_tag="VGB76_19715"
     CDS             1557..>1828
                     /locus_tag="VGB76_19715"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY0101163.1"
                     /translation="MKSMTGFGRGSTAGENFNVSVDLKTVNNRFLDVHLRAGAELSSL
                     ETIIRRRISEQLSRGRVDATIVIDRTSDVSYEMNRPLIAGFIEAMRS"
BASE COUNT          373 a          604 c          511 g          340 t
ORIGIN      
        1 tgctcacgca atccgcagac tttttcgaac gccatcgcac aaactatctc gtctcgcgtc
       61 tcgtctcttc ggcggcggcc atcgaggcgg cggtgacggg cacgttgcgc gacatgctgc
      121 gcgagagttt cacgctcatc gcgtttctct cggcctcttt ttactactcg tggcgtttga
      181 cgctcggctc gcttctcatc gcgcccatca tcggcgtgct cacggccaag ttcggcaaaa
      241 acctgcgcaa cctcgcgcgc gagactttcg aaggcagcca acgcctcgta gacaccgcgc
      301 aggaatcgct cgccaatcat catatcgtca aagcctatcg cggcgaagaa cgcgagcaga
      361 gacgcttcac caccgtcgcg cgccaaatcg tcagcgccaa cctgcgctcg gcgcgcatca
      421 acggcttcgc gccgccgagc atcgaaatga tcggcgtcat cgccgtcgcc atcctgctct
      481 atttcggtcg gcgcgagatc atggcgggca atatgaaccc ggcgcagttt ctcacgttcc
      541 tcttcttcct cttctccagc tacgatccga tgcgcaagct ctcgcgcttg cagaacgctc
      601 tggagatcgc gctcgccgcc gcgcgccacg tctgggaagt gatggacgaa aacttcgaga
      661 cggtcgaacg cgccgaaggt gcggtcgagt tgaaaccgct gcgcgactca atcgagctgc
      721 gcgatgtttc gttcgactac gcgaacaatg aagaacgccc cgtgctgcgc tcgatcaacc
      781 tgcgactccc ggcgggaaaa atggtcgcgc tcgtcggcga atccggcggc ggcaagtcaa
      841 cgctctccaa actcattttg cgtttccacg atcccacgtc cggcgcgatc ctctgggacg
      901 gcacagacct gcgcgacgcg agcctcaaga gcctccgcca gcacatcggc ctcgtcacac
      961 aggagactgt gctcttcaac gacacggtgc gttacaacat ctcttacagt cgccccgatg
     1021 cgaccacggc gcagatcgaa gaggcggcgc gcgtcgcctt cgcgcacgat ttcatcaagg
     1081 acttgccgga aggctacgac accatcgtcg gcgaacgcgg catctttttg tcgggcggcc
     1141 aacgccagcg gctcgcgatt gcccgcgccg tcctcgcaga tgcctccgtg ctcgtgctcg
     1201 acgaagccac ctccgccctc gacgccgaat ccgaacggct cgtgcaacag gcgctctcga
     1261 atttaacgcg caaccgcacc acgctcgtca tcgcgcatcg cctctcgacc atccggcgcg
     1321 ccgactccat cgtcgtcatg gaacgcgggc gcatcatcga acagggcaag cacgaggaac
     1381 tgttaacgca gagcggaact tataagaaac tctatgaact gcaattcgcc gatgaagagg
     1441 aggaggttgt agttagcccg tagccaatcg gctgccgtcc gggtggaaac agggcgagac
     1501 actcaaggag cgagtcgggt ggccgaccgc taaaggctaa ctactgatag ctttttatga
     1561 aatcaatgac cggcttcggg cgcgggtcaa cggcgggcga aaatttcaac gtctccgttg
     1621 atctgaaaac cgtcaacaac cgattcctcg acgtgcacct gcgcgcgggc gcagaactgt
     1681 cgagcctcga aacaatcatc cggcgtcgga tcagcgaaca actatcgcgg gggcgcgtgg
     1741 atgcgaccat cgtgatcgac cgcacgagcg atgtttccta cgagatgaac cggccgctca
     1801 tcgccggttt catcgaagcg atgcgctc
//
LOCUS       DASWNS010000570         5273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_152251, whole genome shotgun sequence.
ACCESSION   DASWNS010000570 DASWNS010000000
VERSION     DASWNS010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152251"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1010..1327
                     /locus_tag="VGB76_19720"
                     /pseudo
     CDS             1010..1327
                     /locus_tag="VGB76_19720"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1421..1855
                     /locus_tag="VGB76_19725"
     CDS             1421..1855
                     /locus_tag="VGB76_19725"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0101164.1"
                     /translation="MLRSHLDSHPGLRGQRDLLLSIPGIGETTAPKLLAEIMDVTLYA
                     SAKQLAAFAGLAPRLHESGSSVKRKARLSKTGAPRLRKALYFPAIAAIKYNPYIKDLS
                     ERLRARGKCPMQVIGAARRKLIHLAYGVLKSGRPFDPQMKTA"
     gene            complement(2336..3532)
                     /locus_tag="VGB76_19730"
     CDS             complement(2336..3532)
                     /locus_tag="VGB76_19730"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEY0101165.1"
                     /translation="MTHKYFTLAAGVALAILLGAASFFPLVPATSAQSTVPRGTTTTF
                     AILAGQAVTSTGLSVVNGDLGISPGTALTGFPPGVITGATHLGDPVAAQALADAQVLY
                     AYLDGLPCGTNLTGQDLGGMTLTPGVYCFDSSAQLTGTLILDAQGECDPLFVFQTGST
                     LTTSVASSIEVINNPCSTNCRGGSNVFFQVGSSATIGTGSQFVGNIVANTSATLNFGA
                     SVAGSVFGLIGAVTLDTNNISACGVSITPTPTPNPSPTATPTPTPTPTITCAPKVTGG
                     GQIPVPDPDSNNQLATGTGRATFGFNSQPEKGCTDGAAKGHFNYVNHVTGLHVNGKVL
                     NSQLVAPNTVRFTGECGTGCTFTVTVQDNGEPGTLDTFGLGVTGTKNEHRSLRVISRG
                     NIQFHF"
     gene            complement(4000..4716)
                     /locus_tag="VGB76_19735"
     CDS             complement(4000..4716)
                     /locus_tag="VGB76_19735"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0101166.1"
                     /translation="MPRNSQPTTLQNQLLAALPSKEYKRLIPHLEPVPLPLMEVLYES
                     GEPIKHVYFPNEGLISLLVVMRDGMPREVGLIGWEGMFGVPVVLGMKAAPTRALIQMP
                     GSAMRMKAQALRDELKRGDALQNILQRYTHAFFTQVAQSAACISSHEVNRRLSRWLVM
                     THDHAPGDVFVMKHEFMAMMLGVTRSVVTRAAVSLQNEKMIRYTRGQVTILDRRRLEA
                     TACECYGAVKKEYERSASTS"
     gene            4938..>5273
                     /locus_tag="VGB76_19740"
     CDS             4938..>5273
                     /locus_tag="VGB76_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101167.1"
                     /translation="MSQRPVEPTGNRLLARLPQEESERLLPHLEHVSFALGEVIYESG
                     GRQSYIYFPTTAIISLLYMMKNGSSAEMGVAGNDGLVGIALFMGGDTVPNRAVVSGAG
                     DALRMKTKVL"
BASE COUNT         1168 a         1463 c         1471 g         1171 t
ORIGIN      
        1 atccgaacca catgcggagg cggataacga taattatgta gttgtggcct tgcgtggaaa
       61 gtgagttaag tgtggatata gtgcgaaagt tcggagcgaa tttcgtgtta aagatttgcc
      121 cccaatcgct gaaaacctct ggcaagcaac agcaattacg gattccggca ccagtatcta
      181 cagttttgtc gcagggtcca acacgcctca tcactcctct gccggataac gcggccattc
      241 ttatcacggg cgttgtaatc gccccgcaaa cactttttat acttctcctc acaatccaag
      301 gaacatccag tgttcctttg tggagcgttc gtgatatgag tctttgccgg cggctttgac
      361 ttcccattgg cgcggactgc cgacaacgcg aataagggct gggcggacag actcaacacc
      421 agcaacatag tggcgaagta tctatttccc tttctcatca tccatctcct tttcagtcgc
      481 tgggaagaat aagttttcga tgaaagaggt gctcatcatg gttgtctaca atgagatgtt
      541 agctactcgg tgacatgcta gggcagcact gcctacttct attttgttgc ttacctaaca
      601 tctgttaaca acaataaacg aaggcgctca atgtatattg tgtcgaatga ttcggccttg
      661 tatgttgacc gcagttcatc aggggtgcat cctgtgcccg tgcgaaccat ggggtgccgg
      721 tgaagctgaa ttcgcctgag gcgaaagagc ctgtgagtta gatcagttcc cggcacccgg
      781 cctgccgcgc cgaggccgga cggtatccta agccgccccc agcggggatg agcttgcctc
      841 cacaaggctg gcccgccgcg agtataactc cgaaagggac gcccgccatg acgatttttc
      901 cgctcggcat cgacatctct aaacgcaagt tcgacgcctg cctcgtccgc caggcaggca
      961 agttccgcca caaggtcttc cccaacactg aggccggctt cgcgcaactc tcgggcggct
     1021 ctcaaagcat cgggttgagc atgtgcatgc ttgcctcgaa gccactggca cttacggtga
     1081 agcactcgcc acccacctcc acgactccgg ccacacggtc agtatcgtca atccggctgc
     1141 tatcaaggct tacgctcaga gccgcctctc ccgcgccaaa acagacaagg ccgacgccac
     1201 actgatcgcg cagttctgtc atgagcggca ccctcccgcc tggcagccag tgccagcaga
     1261 agtgcgcgac ttacaagcac ttgtccgccg cctcgactct ctcatggaga tgcgccggat
     1321 ggaggccaac cggctggagg tggccggcac cgaagcggtc aaagagtctc tggccgggca
     1381 cctcgcttac ctcgatgaag agatcgcccg gactgagcag atgctccgct cgcatctgga
     1441 cagccacccc ggcctcagag ggcagcgcga tctgctgctc tccatccccg gcatcggcga
     1501 gacgaccgct cccaagctgc tcgccgagat catggacgtg acgctttatg cgagcgccaa
     1561 gcagttagcg gccttcgccg ggttggctcc gcggctgcac gagtccggca gtagcgtgaa
     1621 gcgtaaggcg agactgtcaa agacgggcgc gccgcgactg cgcaaggcgc tctacttccc
     1681 cgccatcgcg gccatcaagt acaaccctta cattaaagac ttgagcgagc ggctgcgggc
     1741 gcgcggtaag tgcccgatgc aggtcatcgg ggcggcgagg cgcaagctca tccacctcgc
     1801 ttacggcgtg ctgaagagcg gtaggccatt cgacccgcag atgaaaactg cttgactccc
     1861 aagacggtat ctaactccgc aataaagaga tgaaagccac acgtcgcggt ctaactgccc
     1921 gaatgcctgt ataatgccgc tcctacgctt caaaagccta gcagaatcct cgaccgccaa
     1981 gcgtgcgagg cgtacatcac tacctcagca ctgaactggc gaaaaagaca taattctaat
     2041 tatcgggctc tgattgtttg atgctttttt gaatagcgca tggggattag gttccctgtt
     2101 gggtgaggag cgaaatttac gtcagttctc taaatgaaat gagaagaacg ttattgagag
     2161 gtcgggtcga atgagtcatt agattttgtc cagcttttct tgaagcggtt tcataaatac
     2221 cccgtagcgt ttaggagttg tgatcttgct caggaaatat cagtaataaa aagtccccgg
     2281 aggcgacagg aatcgcctcc ggggacttag tggtttcaca gatcagccgc cacttttaaa
     2341 agtggaactg aatgtttccg cgactaataa cacgtagcga cctgtgttcg ttcttcgtac
     2401 ccgtcacgcc caaaccgaac gtatcgagtg tgccgggttc gccgttgtct tgcactgtca
     2461 cggtgaacgt gcagcccgtg ccgcactcgc cggtgaaccg gacggtgttc ggcgcgacaa
     2521 gctgagagtt gagcaccttg ccgttgacgt gcaggccggt cacgtggttg acgtagttga
     2581 agtggccctt cgcagcacca tccgtgcagc ccttttcggg ctgcgagtta aagccgaagg
     2641 tggcgcgtcc cgtgccggtg gcaagttggt tattgctatc gggatcgggg acaggtattt
     2701 gtccgccgcc cgtcaccttg ggcgcacatg tgattgtcgg cgtaggtgtc ggcgtaggag
     2761 tagccgttgg tgaaggattc ggcgtcggtg tgggcgtgat gctgacgccg caggcggaga
     2821 tgttattggt atcgagcgtc actgctccga tgaggccaaa gacactaccg gccacgctcg
     2881 cgccgaaatt caaggtggcg ctagtgttgg cgacaatgtt tccgacaaac tgagagcctg
     2941 tgccgatggt tgcggaactg ccgacctgaa aaaacacgtt cgacccgcca cggcaattcg
     3001 tactgcacgg gttattgatc acttcaattg acgaagcgac tgaggtcgtg agggtgctcc
     3061 cggtctggaa aacgaagagg ggatcacact caccctgcgc gtcgagaatg agtgttcccg
     3121 tcaactgagc cgacgaatcg aaacaataga cgcccggcgt cagtgtcatc ccgccgaggt
     3181 cttgaccggt caagttagtg ccgcacggca agccatccag ataagcgtac aagacttggg
     3241 catcggctaa agcctgagcc gcgaccggat cgcctaaatg tgtcgctccg gtgatgaccc
     3301 ctggaggaaa gcctgtgagt gcggttcccg gactgatgcc cagatcgccg ttgaccacgg
     3361 ataagcccgt gctcgtcact gcctggccag ccaagatagc gaaggtcgtc gtcgtgccac
     3421 ggggcactgt gctttgcgct gatgttgcgg ggacgagagg gaagaaactc gccgcgccga
     3481 gaagtatggc gagcgccaca cctgctgcga gggtgaaata cttgtgagtc atttgttttc
     3541 cttatctgaa gttaaagccg aggcggagct gcatataatt ctgtctgatt tcgacatcac
     3601 gcggcactta acggcattga ggcatatcat ccggcaagta accttacttc atgtcggcca
     3661 cgctgctcgc tgcggctgaa acagcccggc agttgagcag agtccggcac ttgccgaagt
     3721 tgaattaggg gcgttagaaa agtaatttcg agaaaatgaa cgtgagttct gaggaatata
     3781 cctgcgttcg gtcaagtacg gatgaacggc tcgctattga taccgctact cagttttctc
     3841 tcccagataa cgcgggctta gtcgggtaag catttgttga ggcttgaaga agttgcagat
     3901 taagccaaac taaagcggcc gtctgtgcgg tactacacat atagaaattt atttagtatt
     3961 acttcgcaaa tagctgacgg gtatgtaaga cacgtttcat cacgaagtgg acgcgctccg
     4021 ctcgtactct tttttcactg ccccgtagca ctcacaggca gtcgcctcca gccttcgccg
     4081 gtcgaggatg gtcacttgtc cacgggtata acgaatcatc ttctcatttt ggagactaac
     4141 ggcagccctc gtcaccacgg aacgtgtcac accgagcatc atcgccatga actcatgctt
     4201 catcacaaac acatctcccg gagcgtggtc gtgcgtcatc accagccagc gcgagaggcg
     4261 tctattcacc tcgtgcgaac tgatgcaggc cgccgactgc gcgacttgtg taaagaatgc
     4321 gtgggtatag cgttgcagta tgttctgaag cgcgtcgccg cgtttgagtt cgtctctgag
     4381 cgcctgcgct ttcatacgca tggcgctgcc gggcatctgg atgagcgcac gggtcggcgc
     4441 agctttcata cccagcacca cggggacgcc aaacatcccc tcccagccga tcagtcctac
     4501 ttctctgggc atcccgtctc tcatgacgac taacaatgag attagccctt cgttcgggaa
     4561 atagacgtgt ttgattggct cgccgctctc atagagaact tccatcaatg gcagaggaac
     4621 gggttccaga tgcggaatca gacgcttgta ttctttactc ggcagggccg cgagcagttg
     4681 gttctggagg gtcgtgggct gagagtttct gggcatcaaa atcactcctt gactgtcaag
     4741 tccgttggcg agaccagcag acggcacaag gagatggttc ttactcaaga cccgatgtcg
     4801 aaagatgaat tccgtaaaga acggctgtag taatcgtact actggctgtt caacctcgtc
     4861 tgtgctctaa cacacacata gaatgtataa tcccggcgtc acaccttaca ccttgaaaat
     4921 ggagactttc ctcacacatg tcccaaagac cggttgagcc gacgggaaat cggcttctcg
     4981 cgcggcttcc ccaagaggag tccgaacgtc tcctgcctca cttagagcac gtttcctttg
     5041 cgctcggcga ggtcatctac gaatcaggcg ggcggcaaag ttacatctat tttccgacca
     5101 ccgccatcat ctctctgctc tacatgatga agaacggctc gtcagccgag atgggtgtgg
     5161 cgggaaacga cgggttggtc ggcatcgccc tcttcatggg aggcgatacg gtgcctaatc
     5221 gtgccgtcgt gtcgggtgcg ggcgacgctc tcaggatgaa gacgaaggtg ctg
//
LOCUS       DASWNS010000571         5964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_359353, whole genome shotgun sequence.
ACCESSION   DASWNS010000571 DASWNS010000000
VERSION     DASWNS010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359353"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..267)
                     /locus_tag="VGB76_19745"
     tRNA            complement(191..267)
                     /locus_tag="VGB76_19745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(231..233),aa:Arg,seq:cct)
     gene            complement(313..834)
                     /locus_tag="VGB76_19750"
     CDS             complement(313..834)
                     /locus_tag="VGB76_19750"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY0101168.1"
                     /translation="MDNLKKLIREVPDFPKPGINFYDITTLLKHPEGLRLTVDALSSQ
                     FRDEHVDVVLGIEARGFIFAPALAYHLNAGFVPVRKPSKLPAETAKISYALEYGEDTL
                     EIHRDAVSAGQRVIIADDLLATGGTAKATVDLVEGLGGVVAGLVFVVELEFLPGRSKL
                     AGYDVRSLIKYQS"
     gene            complement(915..1184)
                     /locus_tag="VGB76_19755"
     CDS             complement(915..1184)
                     /locus_tag="VGB76_19755"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY0101169.1"
                     /translation="MARKYFVSGEVQGVGYRFFAQRVAARHQVVGYVRNLRDGRVEVL
                     AEGAPESVEALRHDLAAGPDSGHVEQVEELSLEPTGLYASFRIER"
     gene            complement(1446..4283)
                     /locus_tag="VGB76_19760"
     CDS             complement(1446..4283)
                     /locus_tag="VGB76_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101170.1"
                     /translation="MSESYGRRLFAPMRLPYKISLSRLLLTAWKLCVAGACLFAGQAF
                     SQRTVRARAGSATAARPSPEYLWYEAENMLGFSVDALNNPVSNPSWAMLKRASAPGWG
                     MNGPGVSAEWSQGGESEWNSAAASEDETRASIYQDVSIPRAGQYKVWVRYADWLGKTE
                     NFTLRITQGGREVWRHEFGASDRLAPHDETGMYWGWAFNWDAATTGALAKGSARISLE
                     IERAADARRHVDCFLLTNDPAYLPEGRSKPDFAALRVLREVSARKPRLAPLIEAEGGG
                     PVPDAWRRPTLAGRDFLMPWNIAPEFWPLLDQPPATRPLYPFNAEPLEAFIASYKGAR
                     DVPLFSSPLVVPVIRLEQFTTYMQEGTPFLRHLRETRAPFAVIINYSQATMTEAQGQA
                     AWKLLTGELRDQFIGWISGESIGYVWNAGEVAAMKLTPAMSRRELLEAYRAFYTRTLE
                     RKWSALFHTPTGAMWDKLIAAQATSSTAYAHALGRWGAQMIGLETSAVQPSVAMRIAF
                     SRGAARQYGGSFLYYHAPNFGDTATTFTRLQNFAGPDHFFHTRYGATMGPSLSWYRKT
                     YYLYYMAGASAIYLEQGNDQFFKPAPGEHPFQLNPLGRITNEFVNFASRHKERGTPYT
                     PVAFLLDPAHGWDMTDYPQWAFGVSPPGRHDRALRELFGAAYYPAPVNEGEFATADRQ
                     FFTNGIFGDIFDVLVASDERREAIDSYRIAVAGGRLEWTPAWTEQLKSYVRKGGTLVL
                     NAAQAKGLPEELLGVRLSGASAEADDAKCLLPNEAGANLRGGRYAYERVERRGAEVLM
                     QTPTGEPLVTSFALGRGRVIFCAVPDLLGLDERLTPAAAHLFAHLFAEATPLSIEGDV
                     QRLINRTERGWLVTLINNHGVFKPQQGLAQIDRRASVEVTLSLRGKTIRQAREWTLDA
                     PIEVGRAANVSAVKVVVPPGDLKIIELIEGR"
     gene            complement(4376..5149)
                     /locus_tag="VGB76_19765"
     CDS             complement(4376..5149)
                     /locus_tag="VGB76_19765"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEY0101171.1"
                     /translation="MSTDNVQPPAATRSSLRLADRDERTEQPRSNLPRQFVNFTFYRV
                     RPEWRLLEEAERERGRGEFIEAVEEFRRELLIHTYSITGLRSNADLMIWRIGYSLDPI
                     QEMTARINRTGLGRYVEATQSFLSMTKRSMYIDKDNPEHVEDRLHIVPGQSNYLFVYP
                     FVKTREWYSRSVEERQEMMDEHIRIGSKYRSVKLHTTYSFGLDDQEFVVAFETDEPAD
                     FLDLVQELRETKASSFTLRDTPMYTCRQRPLAACLDALG"
     gene            5483..5806
                     /locus_tag="VGB76_19770"
     CDS             5483..5806
                     /locus_tag="VGB76_19770"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0101172.1"
                     /translation="MTEKRRHPRVKATIAINWGLTTECVKHDRITSFSIGGCFIQTAE
                     VLKPRTLIYISFYLSPNGERLILGEVRYHLEEVGLGVEFLNLNERDKRDFQELVNHYR
                     KTQAA"
BASE COUNT         1168 a         1784 c         1833 g         1179 t
ORIGIN      
        1 agacgaggcc gccgaacaca agaagaaaga ggcgctcggc cagcaatcct tcaacatcgt
       61 gctcggctcg ctgctcggct tcctctccgc ctccgccgtc tcacacatcg gcgcaggcgg
      121 cggcggcggc agcaaagaat gagcggacga tattccgggt tgattaattc gaccagggcg
      181 ggcggaaaac tggtgctccc ggcgcgattc taacgcgcga ccttccgctt aggaggcgga
      241 cgctctatac agctgagcta cgggagcagg caaagggaag ttagcagatg tctcaattcc
      301 agtccagcac gctcaggatt gatacttgat gagcgagcgc acgtcgtagc cggcgagttt
      361 ggagcgaccg ggcaggaact ccagttcgac gacgaagact aatccggcca ccacgccgcc
      421 cagaccctct acgagatcga cggtcgcctt cgccgtgccg cccgtggcga gcagatcgtc
      481 tgcgatgatg acgcgctgcc ctgctgagac ggcgtcgcgg tgaatctcca gcgtgtcctc
      541 tccgtattcg agcgcgtatg aaatcttcgc cgtctcggcg ggcagcttcg aaggcttgcg
      601 caccggcacg aagcccgcgt tgaggtgata ggccagcgcg ggcgcaaaga tgaagccccg
      661 tgcttcgatg ccgagcacca cgtcaacgtg ctcatccctg aactgcgacg agagcgcgtc
      721 taccgtcagg cgcaaacctt ccgggtgctt caagagcgtg gtgatgtcgt agaaattgat
      781 gccgggcttg gggaagtccg gcacttcgcg gatcagtttt ttgagattgt ccatgatgag
      841 ctatcagcta tgagccgcca gctttcagcc aaccagtttt tgttgaaagc tgatggctgt
      901 cggctgaagg ctttttacct ttctatgcga aacgaggcgt ataagcccgt cggttcgagg
      961 ctcagttctt cgacttgttc gacgtggccg gaatcgggac ccgcggcgag atcgtgcctg
     1021 agagcttcga cgctttcagg tgcgccctcg gccaatactt cgacgcgccc gtcgcgcaga
     1081 ttacgcacgt aaccgaccac ctgatggcga gccgccacgc gctgcgcgaa gaaacgatag
     1141 ccgaccccct gcacttcccc gctgacgaaa tatttacgtg ccaccctcat gtgcgaaaag
     1201 tctgaaagcc tcgaagtttt aagatttgca agacgcgcta tcctaaaaga actgctgcga
     1261 aaaatccaat cgcgcctggt tccggcagac cgcgcccaca ccggccactc aaccctctag
     1321 tcttcctgtg cgtctccctt tagcctccct gtgcgtctct gcgcctccgg cgtgagatgt
     1381 agaaaatttc tcgccggagg cgcggagacg cgcaggaagg ctaaaggggt tgtcttgaaa
     1441 acttcttaac gcccttcgat gagttcgatg atcttcaagt cgccgggcgg gacgacaacc
     1501 ttgactgcgc tcacgttcgc ggcacgcccc acttcgatag gcgcgtcgag cgtccattcg
     1561 cgcgcctgcc ggatcgtctt gccacgcagg gagagcgtga cttcgacgct cgcgcggcga
     1621 tcaatttgcg ccaagccctg ctgcggtttg aagacgccat gattgttgat gagcgtgacg
     1681 agccaaccgc gctccgtgcg attgatgagt cgttgcacgt cgccctcaat gctgagcggg
     1741 gtggcttccg cgaacaggtg tgcgaagaga tgcgcggctg ccggggtcag cctttcgtca
     1801 aggccgagca gatcggggac agcgcagaag atgacgcgcc cgcgtcctaa cgcgaacgag
     1861 gtgacgagcg gttcgcccgt cggcgtctgc atcaacacct ccgcgccgcg cctttcgacg
     1921 cgctcatatg cgtaccggcc tccgcgcaaa ttcgcgcccg cctcgttcgg caagaggcac
     1981 ttcgcgtcgt cggcctccgc gctcgcaccg gacaggcgca cgccgagcag ttcttccggt
     2041 agtcccttcg cctgcgcggc gttgaggacg agcgtgccgc ctttgcgcac gtaactcttg
     2101 agctgctccg tccacgcggg cgtccattcg agacgcccgc ccgcgacggc gatgcggtaa
     2161 gaatcaatcg cctcgcgtcg ttcgtcggag gcgacgagta cgtcgaagat gtcgccgaag
     2221 atgccgttcg tgaaaaactg ccggtcggct gtcgcaaatt ctccttcgtt gacgggcgcg
     2281 ggataatagg ccgcgccgaa gagttcgcgc agcgcgcggt cgtggcgtcc gggcggcgag
     2341 actccgaaag cccattgggg atagtcggtc atatcccaac cgtgcgccgg gtcgagcagg
     2401 aaggcgacgg gcgtgtaagg cgtgccgcgc tctttgtggc gcgatgcgaa attcacgaac
     2461 tcgttcgtga tgcgtccgag cgggttgagt tggaagggat gctcgccggg cgcgggtttg
     2521 aaaaattgat cgttgccctg ttcgagatag atggccgacg cgcccgccat gtaatagaga
     2581 taataagttt tgcgatacca ggagagcgaa ggccccatcg tcgcgccgta gcgcgtgtga
     2641 aagaaatggt caggccccgc gaagttttgc aggcgcgtaa aggtggtcgc ggtgtcgccg
     2701 aagttcggcg cgtggtaata gagaaacgag ccgccgtatt ggcgcgccgc gccgcgcgag
     2761 aaggcgatgc gcatggcaac cgaaggctgc acggcggaag tctcaaggcc gatcatctgc
     2821 gcaccccagc gaccgagcgc gtgcgcgtaa gcggtgctcg aagtggcctg cgcggcgatg
     2881 agtttgtccc acatcgcgcc ggtcggcgtg tggaaaagcg cgctccattt gcgttcgagc
     2941 gtgcgcgtgt agaaggctcg ataggcttcg agcaattcgc ggcgcgacat ggcgggcgtg
     3001 agtttcatcg ccgcgacttc gcccgcgttc cacacgtagc cgatgctctc gccggaaatc
     3061 caaccgatga attgatcccg gagttcgccc gtcagaagtt tccaggcggc ctgcccctgc
     3121 gcttcggtca tggtcgcctg actgtagttg atgatgacgg cgaagggggc gcgcgtctcg
     3181 cgcaggtggc gcagaaaagg agtgccctcc tgcatgtagg tcgtgaattg ttcgagacgt
     3241 atgaccggca cgacgagcgg cgaggagaag agcggcacgt cacgtgcgcc tttataactg
     3301 gcgatgaagg cttcgagcgg ttcggcgttg aaaggataga gcgggcgcgt cgcgggcggt
     3361 tggtctaaga gcggccagaa ctccggtgcg atgttccacg gcatcagaaa atcgcgcccg
     3421 gccaacgtcg gcctacgcca cgcgtcgggt accgggccgc ctccctccgc ttcgatgagc
     3481 ggcgcgaggc gtggtttgcg tgcggagact tcgcgcaaca cgcgcaacgc agcgaaatcg
     3541 ggtttgcttc gtccttcggg aaggtaggcc ggatcgttcg tgagtaagaa gcaatcgacg
     3601 tggcggcgcg cgtctgcggc gcgttcgatc tcaagagaga tgcgcgccga gcctttggca
     3661 agcgcgcccg tcgtcgctgc gtcccagttg aacgcccagc cccagtacat ccccgtctcg
     3721 tcgtgcggcg cgagacggtc tgacgcgccg aactcgtgac gccagacttc acgcccgccc
     3781 tgcgtgatgc gcagagtgaa attttccgtc ttgccgagcc agtcggcata acgcacccag
     3841 actttgtact gccccgcgcg cgggatggac acgtcctgat agatgctggc gcgcgtctca
     3901 tcctcactcg ccgccgcaga gttccattcg ctctcgccgc cctgcgacca ttccgcagac
     3961 acgccgggac cattcatccc ccaccccggc gcgctcgcgc gtttcaacat cgcccacgaa
     4021 ggattcgaga cgggattgtt caacgcatcg accgagaagc cgagcatgtt ctccgcttca
     4081 taccagagat attcgggcga cggacgggca gccgtagcag aaccggcgcg cgcgcgcacg
     4141 gttctttgcg aaaaagcctg ccccgcgaag agacacgcgc ccgcgacgca aagtttccac
     4201 gccgtcaaca gcaaccgcga caggctgatc ttgtatggca gacgcatcgg cgcaaaaagg
     4261 cggcgaccgt acgactcact catcgtctcg acagtcgccg cgcttttgac tcacggaaaa
     4321 gttcaggggt ttcgccagcg gcagtttgcg ctctcggcca tcacttgcac gcgccttaac
     4381 cgagcgcgtc gaggcacgcg gcgagcgggc gttgacgaca ggtgtacatc ggcgtgtcgc
     4441 gcaaggtaaa gctgctggct ttggtttcgc gaagttcctg cacgagatcg aggaagtccg
     4501 ccggttcatc cgtctcgaaa gcgacgacga actcctgatc gtcgaggccg aacgaatagg
     4561 tcgtgtgcaa tttcaccgag cgatatttcg agccgatgcg gatgtgctcg tccatcatct
     4621 cctgccgctc ttcgaccgaa cgtgaatacc actcgcgcgt tttcacgaac gggtagacga
     4681 acaggtagtt cgactgtccg gggacgatgt ggaggcggtc ttcgacgtgc tccgggttgt
     4741 ccttgtcaat gtacatcgag cgtttcgtca tggagagaaa cgattgggtc gcttcgacgt
     4801 agcggcctaa gccggtgcgg ttgatgcgcg ccgtcatctc ctgaatcgga tcgagcgagt
     4861 agccgatgcg ccagatcatc aagtcggcgt tggagcgcaa gcccgtgatc gagtaggtgt
     4921 gaatcaacaa ctcgcggcga aactcttcga ccgcttcaat aaactctcct ctgccgcgtt
     4981 cgcgctccgc ctcttcgagc agacgccatt cgggacgcac ccggtagaaa gtgaaattca
     5041 cgaactggcg cggcaagttt gaccgtggct gttcggtacg ctcgtcgcgg tcggcgagac
     5101 gaagagaaga gcgtgtagct gcgggaggct gcacgttgtc tgtgctcata gtgcgttcat
     5161 cctcaaaata attgcgttga atcaaacgcc tctctcgcct gtcgggaaaa tttgtgaagc
     5221 gtcagcttca gagcggcgcg gggctattgt gctgcgtcgc gtgggagatt gcaaatggcg
     5281 acgcagcctc cgcgtcaggt tcgcggcgcg ctccggcgtg agccatgttt tctccgcacg
     5341 aaacaccctt gcgccatgtg cgacaacaaa aaattcgccc gccgggcaac aaaaattttc
     5401 gctccgcgac aaattcgttt aagatggagt tgtaggttca agcggcatgc gtcatgcgcc
     5461 tcatgtgccg ccctcatatt atatgactga aaaacgcagg catccccgcg tgaaagccac
     5521 aattgcgatt aattgggggc taacgacgga atgtgtcaaa catgatcgca tcacgagttt
     5581 cagcatcggc ggctgtttca ttcaaaccgc cgaagtcttg aagccacgaa ctctcattta
     5641 tatcagcttc tatctctcgc ctaacggtga gcgacttatt cttggcgaag tcaggtatca
     5701 cctggaagag gtggggttgg gagtcgagtt tctcaatctc aacgagcgcg acaagagaga
     5761 ctttcaggag cttgtcaatc attatcgcaa gacgcaggcc gcataagttc ccgcgcggca
     5821 cttacgactt tcgattcgta gccgaacacg ctcccagcgt cgcgcgggcg tacaggtgtg
     5881 tcgtgcaaac gccctatatg ctccgctttt aaacgcgcgc gcctctaagt tatactcgcc
     5941 gcgttttcca tcccgacctg cttt
//
LOCUS       DASWNS010000572         3239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_306630, whole genome shotgun sequence.
ACCESSION   DASWNS010000572 DASWNS010000000
VERSION     DASWNS010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306630"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VGB76_19775"
     CDS             complement(<1..722)
                     /locus_tag="VGB76_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101173.1"
                     /translation="MPDSETHTVKEADAAAAAGASRVWARPHVVVPLALYALVTWLTN
                     AFYMGDTYFYSSVVARVLGGESYHIAEDIGLYSLWDFGHLLWRPLGVLVFPLARPLAH
                     ASVGADLNPAVTYMFVALNWLAGLSSVGLLYGVLRRVCDKTWAVNVTIVAFIFAQAFL
                     NYTQTGCSYIPSLSLLLLGIYLLVRDGERVERPVLTALLAALALTGAVGLWVPFVWVV
                     PAAVATPLLLFGWQSGQRWRLSV"
     gene            complement(761..2455)
                     /locus_tag="VGB76_19780"
     CDS             complement(761..2455)
                     /locus_tag="VGB76_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101174.1"
                     /translation="MRTQSLVPLIAYALATWLTNARIMGDTVYYVDSILRVRAGGQKF
                     SYWEPLGNYSFFEFGHLLWRPFGYLVAVLCEPLAGVFGGAAATDGRARVTLVLVGINW
                     LAGLACVLLWQGLLRRLRVSGWASVAATISLICAHGFLNFAQTGSSYIPALASLLLGM
                     YVLARAGARVEAGSVWPAALLAGGALGCAVGFWFLFALAIPAALLLPLIFFGDERRRR
                     LLIAATAVSCAAFVLSMYLFVVMLGLGITDVATFKAWVVSSSHGLANNRGVPQVVLGL
                     ARSFIEIGNDHLIFKRFLLRDPYNVVSLFDLFRLSLWKLGLFYLAFAAVVFALLRERA
                     GGWRVLALFGLSGAPVLAFAGLWQGTPTERYLPFYPAFFLALAYALRSGARARWVRPV
                     VLAFMVLSVVSNLSALSVRRLNRVPEAVTARTRELMPLLKPQSIVVEVLEELNELQWA
                     YPLHPLNRVLRVYSAISLGATNTAEWREDFARQTLLVWRDGGDVWLSKRVLEPRPRAE
                     SSWVEGADPRVRWTDIPAFFSQLEAGQTTGDRDGFVLVARTPRNEQFLSALAAQTK"
     gene            complement(2580..>3239)
                     /locus_tag="VGB76_19785"
     CDS             complement(2580..>3239)
                     /locus_tag="VGB76_19785"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0101175.1"
                     /translation="NNYRNWMYKRMEPYIGQRILEVGAGIGNFTELLLDRELVVASDK
                     YQMCVDYLQQRLGSQLKGAPVQMDLAAPPESALHKFNFDTVICINVLEHVEDDAKALA
                     YMHSVLVPGGRVILLVPAFQFLYGTIDRAIEHYRRYTKKDLLPRMREAGFEVESAFYM
                     NVIGMAGWFWNNRVRKIKEENAAQIQVFDKFIAPWAERVERTLQPPFGLSLIAIGRKA
                     K"
BASE COUNT          680 a         1082 c          996 g          481 t
ORIGIN      
        1 accgagagcc gccaacgctg cccgctctgc cagccgaaca gaagcagcgg cgtcgccacc
       61 gccgccggga cgacccacac aaacggaacc cacagcccga ccgcgccggt cagtgcgagc
      121 gcggcgagca aggccgtcag cacgggtcgc tcgacgcgtt cgccgtctct caccagcagg
      181 tagatgccga ggaggaggag cgatagagaa gggatgtagg agcaacccgt ctgcgtgtag
      241 ttcagaaagg cttgcgcgaa gatgaaggcg acgatggtga cattgaccgc ccacgttttg
      301 tcgcacacgc gccggagcac gccgtagagc agcccgacgc tcgacagccc cgcgagccag
      361 ttgagcgcga cgaacatgta ggtgaccgca gggttcaggt ctgcgccgac cgaggcgtgc
      421 gccagcggac gcgcgagcgg aaagacgagc acgccgagcg gacgccacag caggtggccg
      481 aagtcccata gcgaatagag gccgatgtcc tccgcgatat gatagctctc gccgccgagc
      541 acgcgcgcca cgaccgaact atagaaatag gtgtcgccca tgtagaaggc gttcgtcagc
      601 cacgtcacca gcgcgtagag ggcgagcggc acgacgacgt gcggcctcgc ccagacgcgc
      661 gacgcgcccg ccgccgctgc cgcgtcggct tcctttaccg tgtgtgtttc agagtcgggc
      721 atgtcggaca aaatcctccg cctgcgagac gcggcgcgcg ttatttcgtt tgcgccgcga
      781 gcgcggacaa aaactgttcg ttccggggcg tgcgcgcgac gagcacgaag ccgtcacggt
      841 cgccggttgt ctggcctgct tcgagttgcg agaagaaggc aggaatgtct gtccagcgca
      901 cgcgcgggtc tgcgccttca acccacgacg actctgcgcg cgggcgcggc tccaacacgc
      961 gcttcgagag ccagacgtcg ccgccgtcgc gccacacgag gagcgtctgg cgcgcgaaat
     1021 cctcacgcca ctccgccgtg ttcgtcgcgc ccagagagat cgccgaatag acgcgcagca
     1081 cgcggttgag cgggtgcagc gggtacgccc actgcaactc gttcaactct tcgagcactt
     1141 ccaccacgat gctctgcggc ttcaacagcg gcatgagttc gcgcgtgcgc gccgtcaccg
     1201 cttccggcac gcggttcaac cttctcacag acaacgcgct caggttcgac acgacggaca
     1261 gaaccatgaa cgcgagcacc acggggcgca cccagcgcgc ccgtgcgccg gagcgaagcg
     1321 cataggccaa cgctaaaaag aaggccggat agaacggcag gtagcgttcg gtcggcgttc
     1381 cctgccacag ccccgcgaag gccagcaccg gcgcgccgct caagccaaac aacgccagca
     1441 cgcgccagcc gcccgcgcgt tcgcgcagca acgcgaagac gactgccgcg aaggcgaggt
     1501 aaaacagccc caacttccac aggctgagcc ggaagagatc gaacagcgag acgacgttgt
     1561 aagggtcgcg caacaggaac cgcttgaaga tgagatgatc gtttccgatc tctatgaacg
     1621 aacgcgccag tccgagcacg acctgcggca cgccccggtt gttcgccagc ccgtgcgacg
     1681 aagagacgac ccatgccttg aacgtcgcaa catccgtaat ccccagcccc agcatcacga
     1741 cgaagaggta catcgagagc acgaacgcgg cgcacgaaac ggcggtcgcg gcgatgagca
     1801 gacgccgccg tcgctcgtcg ccgaagaaga tgaggggcag caagagtgcc gccgggatcg
     1861 ccagcgcgaa caggaaccag aacccgaccg cgcacccgag cgcgcctccc gcgagcaacg
     1921 ccgcaggcca cacgctcccc gcttccacac gcgctcccgc gcgcgccagc acgtacatcc
     1981 ccagcaacaa cgacgcgagc gcggggatgt aagacgaacc ggtctgcgcg aagtttaaaa
     2041 agccgtgcgc gcagatgaga ctgatcgtgg ccgccacgct cgcccaaccg ctcacgcgca
     2101 gacgccgcaa caagccctgc cacaacagca cacacgcgag tccggccagc caatttatcc
     2161 cgacgagcac gagcgtcacg cgtgcgcgcc cgtccgtcgc cgccgcgccg ccgaaaactc
     2221 ccgcgagcgg ttcgcacaag acggcgacga gatagccgaa gggtcgccag agcagatgcc
     2281 cgaactcgaa aaaggagtag ttgccgagcg gttcccagta ggagaacttc tgcccgcccg
     2341 cgcgcacgcg caagatcgaa tccacgtagt agaccgtgtc gcccatgatg cgcgcattcg
     2401 tcagccacgt cgcgagcgca taggcgatga gcggcacgag gctctgcgtg cgcaaaaatt
     2461 ttcgccaaaa gtttcccacc gggtctttgt ttaatggacg agcttttcgc cggtcggtca
     2521 cggcgaatca aggaactcgt agcggctaga gggtcgaact gtccgcgcca actttgcctc
     2581 tacttcgctt tacgcccgat ggcgatgagc gacaggccga agggcggctg gagcgtgcgc
     2641 tccacgcgct ccgcccacgg cgcgatgaat ttatcgaaga cctgtatctg cgccgcgttc
     2701 tcttccttga tcttgcgcac gcggttattc cagaaccaac cggccatccc gatcacgttc
     2761 atgtagaacg cgctctccac ctcgaacccg gcctcgcgca tcctcggcag taaatctttt
     2821 ttcgtgtaac ggcgataatg ctcgatggcg cgatcgatcg tgccgtagag aaattgaaac
     2881 gcgggaacga gcaggatgac gcgcccgccc ggcacgagca ccgaatgcat gtaagcgagt
     2941 gccttcgcgt cgtcttccac gtgttcgagc acgttgatgc agatcaccgt gtcgaagtta
     3001 aacttgtgca gcgcgctctc tggcggcgcg gccaggtcca tctgcacggg cgcgcccttg
     3061 agttgcgagc cgaggcgttg ctgcaagtag tccacgcaca tctggtattt gtctgacgcg
     3121 acgacgagtt cacgatccag caggagttcc gtgaagtttc cgatccccgc gccgacttcg
     3181 aggatgcgct ggccgatgta cggctccatg cgtttgtaca tccagttgcg ataattgtt
//
LOCUS       DASWNS010000573         9752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_462147, whole genome shotgun sequence.
ACCESSION   DASWNS010000573 DASWNS010000000
VERSION     DASWNS010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462147"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VGB76_19790"
     CDS             complement(<1..1023)
                     /locus_tag="VGB76_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101176.1"
                     /translation="MEERQPTEEQDGGGRVVYSRLPSEAGEARPPVRTERGAGSLSSF
                     VHEPLAPTLPTLLVAFVLLVILVFALGYLSMREVESVSQQVQELDQQLADRMQALLTL
                     RSALTQLDNEARERGTKEAGGGIINPFDLKLRRARGEVEKQLYLFDHLPLAQTERGQT
                     FRRAAADYIESTNDAEQYSIEGFKKFRPIEDHVDAFTGEINQQQFELRNRRAALVGES
                     ARRIRMLTWISVLVGALVAAATIWEVQRRFRQMRKSLEESRRERLFSSQMLEGMVSAV
                     AAIDGGGALRSANAAFFQLFPGASVGTSVHDAFAAPDALKMFAAATASRVEHVTYQGR
                     WTLSDTE"
     gene            complement(1152..3890)
                     /gene="polA"
                     /locus_tag="VGB76_19795"
     CDS             complement(1152..3890)
                     /gene="polA"
                     /locus_tag="VGB76_19795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY0101177.1"
                     /translation="MPRTSSSPNGTTPPERVFLIDSFSHIFRAFFAPMGGRMEPLTSS
                     SGQVTQAVFVFTNMLRKLLADEQPHYLAAIFESEVPTFRHEMDVGYKANRDGMPEELT
                     SQIPFIMRVCEAYNVPILSVPGFEADDVIGALAVQAAQAGLQAVIVSNDKDMCQLVSD
                     PLIVCMRQNSQNVKRKEPVPPIEWCDEAWVENKFGVPPAQIVDLLGLMGDAVDNIPGA
                     PGIGAKGAVQIVKQFGSIENALARWEEVKHKTYRESLRDNAPLIRQSLELATIKTDVN
                     IKLDLDQLRAKPPDRLAAYKLFRELEFQTLTREFADAAAGLEGVETHARRYTRITTRE
                     ELDNLVRKLWDVDHWSFAVADSTPAGAGQQGASRDQAAPTGVAISTAAHTSAFIDFEA
                     FEGGAASAVEPLRDVLANGLLSKSVHDLKRAVALLAQLGVEIEGVTDDTLLAAYLLDP
                     IRSKYDLADLAREAVNGDGWTEAVEGWTEAHWRAAEAADFTVQTADVLHGRILEQGLE
                     SIYNEIELPLAPLLHRMEHAGLRVDTGVLGELSAFFGAELEKLTARIYKEAGREFKIT
                     SPKQVGEVLEELNISSGRKTSTGQVSTNRSVLDELALKYELPRLIIEYRELDKLKTTY
                     TDALPALLGADGRIHSQLNQTVTATGRLSSSEPNLQNIPIRTELGRRIRRAFIPDKGS
                     VFVSADYSQLELRLLAHITRDPVMLDAFQKGEDIHTRTAQLVFKAKTKEELKEKRRFA
                     KIVNFAIAYAVEPFGLSARVGISRKEARQVIDDFYATYKGVREYMDKIPEQAREQGLV
                     RSIYGRIRPLPTINDRNGQIRARAEREAINMPIQGTASDIVKIAMLKVDEALRRENLS
                     AQMVMQVHDELLIEAPKREAEQVAALLHREMESAVELDVPLDVEVGTGTNWMDVKK"
     gene            complement(4052..4438)
                     /locus_tag="VGB76_19800"
     CDS             complement(4052..4438)
                     /locus_tag="VGB76_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101178.1"
                     /translation="MVDDGRELFGNFTPQPPPHIGAQRNGFLALAVYDWRTNGGNADG
                     VIDKQDPIFSSLRLWQDTNHNGVSEAAELHSLPSLQVVSLRLDYKESKRTDEHGNRFR
                     YRAKIDAAGGAKAGRWAWDVFLLNAP"
     gene            complement(4378..5220)
                     /locus_tag="VGB76_19805"
     CDS             complement(4378..5220)
                     /locus_tag="VGB76_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101179.1"
                     /translation="MNTHLVLSHLRNLKSTLVAVVLLAPFVAVCAVGIVSSTAQSTQD
                     DSKEERKFENRIPAHVPLKVKLKNEQSFKNPGNKKWLRELEIEVKNTGSKPIYYLCLS
                     VKMPDVIVQGYQYGMQVIYGRWELVKFTNPIRSDDVPILPGESVVLKVSEKMVKGYES
                     FRDKENRDDPKKVQFNLVLINFGDGTGLITYQGVPFPIQPEQSLNEVRPPGQPEHSPP
                     APDQQATDSRFKLLTSNFSFMPASFLRAKFSPAKDCPVSSTAAWWTTGENCSATSRRN
                     PRRT"
     gene            5533..6519
                     /gene="lpxC"
                     /locus_tag="VGB76_19810"
     CDS             5533..6519
                     /gene="lpxC"
                     /locus_tag="VGB76_19810"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY0101180.1"
                     /translation="MSVATVYGCFTVADDARPDLKRVKVERINLLETLIKQTTLAAPA
                     ETSGMGLHTGVRVQVRLLPAPPDTGYIFLRTDLDNFEIPATVEHVAHSSYATTLMRKG
                     VTLSTVEHLLAALRGGGVDNAYIEVDNLELPIMDGSAEVFTELIERVGIVEQPATRLA
                     LRVCKKVSVAEGKRRMSVEPSDGFEIDCMIDFPHPLIGTQRMRFDALEASAFGREIAS
                     ARTFGFTDEIEALRRANLIRGGSIDNAIVLTPEGMLNETGLRSRDEFVRHKILDIIGD
                     LALVGMPLLGLVRAERSGHLFHAMLMTKLLRDDTAWEVVDMPVGAVVHAHGD"
     gene            6680..7237
                     /locus_tag="VGB76_19815"
     CDS             6680..7237
                     /locus_tag="VGB76_19815"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEY0101181.1"
                     /translation="MSTAKTEKLRRFSLDALTQTLASLKNAPTQSELDGLLRMLEPEP
                     EELREHLGFKPNTYTRHRLFRNDLCEMLVLCWLPGQCTSIHDHNGSYGAIRICEGAME
                     EEIFALNEAGEVVYETTHRRAAGEVTGTGVPDIHRIGNAEGSTERMVTIHVYAPPLRV
                     INTYRLGSAEVGECWPDDPANPANG"
     gene            complement(7367..8176)
                     /locus_tag="VGB76_19820"
     CDS             complement(7367..8176)
                     /locus_tag="VGB76_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101182.1"
                     /translation="MNKRREMRASLASLASARPARAGFNSQTLSRFAVSLLCALSLYA
                     GALPCYGIARYGNVSAAQASARPRETKAIQAGEVVVGLNEQIFNALIEAMFTLPNPPS
                     FPLARGNSSEGGSRGCASEIALQREMGGTRTAVRFQAGRIHAPVAFSGSYSAPLIGCL
                     KFEGWAETNLNLSFDQAKQALTARVEVKAVHLKKIPALLGEGITGLVQDALDARVNPI
                     EILRAEQLSARLPMTKTGSGASLRLRAREVRHEVLPNELRLRIVYEFVREE"
     gene            complement(8246..9229)
                     /locus_tag="VGB76_19825"
     CDS             complement(8246..9229)
                     /locus_tag="VGB76_19825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY0101183.1"
                     /translation="MAEQRHYTEFTRFLKLEAEAIAETAARIARAEVERAVELLASCA
                     GKVIVVGVGKSGNVAQKIAATLTSTGTLAVFLHPSDALHGGLGIVAPADILIMISNSG
                     ETDELLAMLPFLKHRQTAIIAVVGNLRSTLAARADVVLDASVDKEACPLNLAPTTSTT
                     VALAIGDALALTLMDCKKLTPEDFALNHPAGRLGKRLMLKVGDVMHGGAENPTIASDA
                     PLLEVVQAISRGGLGAVNVLEGEGQLAGIITDGDLRRALGRSRLADLETLTSRDVMTP
                     RPIKTSPDTLAYAALQLMEDRPSQISVLPVVDEAGTCVGLLRLHDIIRSGL"
     gene            9487..9678
                     /locus_tag="VGB76_19830"
     CDS             9487..9678
                     /locus_tag="VGB76_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101184.1"
                     /translation="MEEQDERHGELPRQQAGGETPSSDTGDIHRPRPAVERKTEDGTA
                     GQGLGQVSGATREDAKEKG"
BASE COUNT         1787 a         2942 c         3087 g         1936 t
ORIGIN      
        1 ctctgtgtcg ctcaaagtcc aacgcccttg atacgtgacg tgctcgacgc gtgaggcggt
       61 ggccgccgcg aacatcttca aggcatcggg cgcggcgaaa gcatcgtgga cggaggtgcc
      121 cacggatgcg ccggggaaaa gctggaagaa ggccgcgttg gcactgcgga gcgcgccgcc
      181 cccgtcaatg gccgccaccg cgctgaccat gccttcgagc atctgcgaac tgaacagacg
      241 ctcacgccgc gactcttcga ggctcttacg catctgccgg aaacgcctct gtacttccca
      301 gatcgtcgcc gccgcgacga gcgcgcccac cagcacggag atccacgtca gcatgcgtat
      361 gcgccgcgcc gactcgccga ccagagccgc gcgccggttg cgcaactcga actgttgttg
      421 attgatctcg ccggtgaagg catcgacgtg atcttcgatg ggacgaaact ttttgaagcc
      481 ttcgatggaa tactgttcgg cgtcgttcgt gctctcgatg tagtcggctg ccgcgcggcg
      541 aaacgtctgg ccgcgctcgg tctgcgcgag cggcaggtgg tcgaagagat agagctgctt
      601 ctccacttcg ccgcgtgcgc gcctgagttt caggtcgaac gggttgatga tgccgccgcc
      661 cgcctccttc gtgccgcgct cgcgcgcttc gttgtcgagt tgcgtgagcg cgctgcgaag
      721 cgtgagcagg gcttgcatgc ggtcggccag ttgttgatcg agttcctgca cttgctggct
      781 gaccgactcg acttccctca tgctcaaata gccgagcgca aagacgagta tcacgagcaa
      841 cacgaaggcg acgagcaggg tcggcagcgt gggcgcgagc ggctcatgca cgaacgacga
      901 caggctaccc gccccgcgct ccgttcgaac cggggggcgc gcttctccgg cctccgaagg
      961 caagcggctg taaaccacgc gcccgcctcc atcctgctcc tctgtgggtt gccgttcttc
     1021 cataaaacag tgacaagtga caagtgacga agcaataaag tgccgacgcg tcgaagtaac
     1081 taagcgagga agtgacgaag cgacaagtca aagacaggtt ttagtcttaa ctcgtcactc
     1141 gtcacccgtc actacttctt aacgtccatc caattcgtgc ccgtgccgac ttcgacatcg
     1201 aggggcacgt cgagttcgac ggctgactcc atctcgcggt gcaggagcgc ggcgacctgt
     1261 tcggcctctc tcttcggcgc ttcgatcagg agttcgtcgt gtacctgcat gaccatctgc
     1321 gccgagagat tttcgcgccg cagggcttcg tcaactttga gcattgcgat cttgacgatg
     1381 tcgctggccg tgccttgaat gggcatgttg atggcttcgc gttcggcgcg ggcgcgaatc
     1441 tggccgttgc ggtcgttgat ggtcgggagc gggcggatgc gaccgtagat cgagcgcacg
     1501 agtccctgct cgcgcgcctg ttcgggaatc ttgtccatgt actcgcgcac gcctttgtag
     1561 gtggcgtaga aatcgtcgat cacctgccgc gcctctttgc gcgagatgcc gacgcgcgcc
     1621 gacagcccaa agggttcgac ggcgtaggcg atggcgaagt tgacgatctt ggcgaagcgg
     1681 cgtttctctt tgagttcctc tttggtcttg gccttgaaga cgagttgggc ggtgcgcgtg
     1741 tggatgtctt cgcctttctg gaaggcgtcg agcatgacgg gatcgcgcgt gatgtgcgcc
     1801 agcaggcgca gttcgagttg ggaataatcg gcggagacga agacggagcc tttatcggga
     1861 atgaaggcgc gacggatgcg gcgaccgagt tcggtgcgga tggggatgtt ttgcaggttg
     1921 ggttcggaac tcgaaagcct gcctgtcgcc gtcacggtct gattgagttg gctgtggatg
     1981 cgcccgtccg cgccgagcag tgcggggagc gcgtccgtgt aggtcgtctt gagtttatcg
     2041 agttcgcggt attcgataat gaggcgcggg agttcgtact tgagcgcgag ttcgtcgagc
     2101 acggagcggt tcgtagagac ttgccccgtc gaagtcttgc gcccggaact gatgttcaac
     2161 tcttcgagca cttcgccgac ctgcttggga gaagtgatct tgaactcgcg ccccgcttcc
     2221 ttgtagatgc gcgccgtcag tttctccaac tccgcgccga agaaggccga caactcgccg
     2281 agcacgcccg tgtccacgcg caagcccgcg tgctccatgc gatgcaggag cggcgcgagc
     2341 gggagttcga tctcgttgta gatggattcg agtccctgtt cgagaatgcg cccgtgcaga
     2401 acgtctgcgg tctgcacggt gaagtccgcc gcttcggcag cgcgccagtg cgcttccgtc
     2461 cagccttcga cggcttccgt ccaaccgtcg ccgttgacgg cttcgcgcgc gaggtctgcg
     2521 aggtcgtact tgctgcggat cggatcgagc aggtaggcgg cgagcagcgt gtcgtcggtg
     2581 acgccttcga tctccacgcc caactgcgca agcagcgcga ccgcgcgttt gagatcatgt
     2641 acagacttcg agagcaggcc gttggcgagc acatcgcgca aaggttcgac ggcggaggcc
     2701 gcgccgcctt cgaacgcctc gaagtcgatg aaggcggacg tgtgcgcggc ggtcgagatg
     2761 gcgacgccgg tgggcgctgc ttgatcgcgg ctcgcgccct gctggcctgc gcccgccggg
     2821 gtcgagtcgg cgacggcgaa actccagtga tcaacgtccc agagtttgcg cacgaggttg
     2881 tcgagttcct cgcgcgtcgt gatgcgcgtg tagcggcgcg cgtgcgtctc cacgccttcg
     2941 agtccggcgg cggcgtcggc aaattcgcgc gtgagcgtct gaaattcgag ttcgcggaaa
     3001 agtttgtagg cggcgagacg gtcgggcggt ttggcgcgca gttgatcgag gtcgagtttg
     3061 atgttgacgt cggttttgat ggtggcgagt tcgagcgatt ggcgtatgag gggcgcgttg
     3121 tcgcgcagag actcgcggta agtcttatgc ttaacctctt cccagcgcgc gagcgcgttc
     3181 tcaatcgagc cgaactgttt gacgatctgc accgcgccct tcgcgccgat gccgggcgcg
     3241 ccggggatgt tgtcaaccgc gtcgcccatc aggccgagca ggtctacgat ctgcgcgggc
     3301 ggcacgccga acttgttttc gacccacgcc tcgtcgcacc attcgatggg cggcacaggc
     3361 tccttgcgtt tgacgttctg cgagttctgc cgcatgcaga cgatcagcgg atcgctgacg
     3421 agctggcaca tgtccttgtc gttcgagacg atgacggcct gcaagccagc ttgcgccgcc
     3481 tgcacggcga gcgcgccgat cacgtcgtcg gcctcgaagc cgggcacgct caggatgggc
     3541 acgttgtagg cttcgcagac gcgcatgatg aaaggtatct gcgaagtgag ttcttccggc
     3601 atcccgtcgc ggttcgcttt gtagcccacg tccatctcgt gccggaaggt gggcacttcg
     3661 gactcgaaga tggcggcgag atagtgtggc tgctcgtctg cgagaagttt gcgcagcatg
     3721 ttcgtgaaga cgaagacggc ctgtgtgacc tgaccgctgc tgctcgtcaa aggttccatg
     3781 cgcccgccca tcggcgcgaa gaacgcgcgg aagatgtgcg agaacgaatc tatgagaaag
     3841 acgcgttcgg gcggtgtcgt cccgtttgga gaagatgatg tgcgtggcat gcgcgcttga
     3901 tgatatgaaa tgccgtgacg cgtgacaagt gagttgacag gccgcgagcc ggacgccgcg
     3961 cgcccgcaag cctctgacat aaagagaacc gcccgcaggt tcgggtgaga cttgcgggcg
     4021 gtggctcaga aaatagaccg tggcagcgat ctcatggcgc gttcagcagg aacacgtccc
     4081 acgcccagcg acccgccttc gcgcctccgg cggcgtctat ctttgcacgg tagcggaacc
     4141 ggttgccgtg ctcgtccgtg cgcttcgact ccttgtaatc gagtcgcagg ctgacaacct
     4201 gcaaggaggg caaactgtgc aactccgccg cctccgagac gccgttgtga ttcgtgtcct
     4261 gccacaagcg gagcgacgaa aagatcgggt cttgtttatc gatcacgccg tccgcgttgc
     4321 cgccgttcgt tctccaatca tagacggcga gggcgaggaa gccgttgcgc tgcgcgccta
     4381 tgtgcggcgg gggttgcggc gtgaagttgc cgaacagttc tcgcccgtcg tccaccatgc
     4441 cgccgtgctt gagacgggac agtccttcgc cggagaaaac tttgcccgca aaaaacttgc
     4501 gggcatgaag gagaaattcg atgtgagcag cttgaagcgt gaatccgtcg cctgttggtc
     4561 gggagcgggc gggctgtgtt cgggctgtcc cggcggacgc acttcattca gtgattgctc
     4621 tggctgaatt gggaatggca cgccttgata cgttattagt cctgttccgt ccccgaaatt
     4681 gataagtacc aagttgaact gaaccttttt aggatcgtcg cgattctctt tatctctaaa
     4741 gctttcatat ccttttacca ttttctcgga aaccttcagg acgacgctct cgccgggcag
     4801 aatcggcacg tcatcggatc ggatcgggtt ggtaaatttc actaattccc atctgccgta
     4861 aattacttgc atgccatact gatacccctg aacgatgacg tccggcattt taacactcaa
     4921 gcacaggtag tagatcggct tgctgcccgt gttcttcact tcgatctcca actcgcgcag
     4981 ccacttcttg ttcccggggt tcttgaagga ctgctcattc ttgagcttca ccttgagcgg
     5041 cacatgcgcc ggaatcctgt tttcaaactt cctttcctct ttggaatcat cttgtgtaga
     5101 ttgcgccgtg ctcgacacga tgcccaccgc acagacggcc acgaacggcg cgagcagtac
     5161 gacggcgact agagttgact tgagatttcg caggtgagag aggacgaggt gcgtgttcat
     5221 gtgactgact ccttcgggtg gccgcgttga ggtgagaggc gttcactgac tacgtacggg
     5281 cagcttaatc ctgcccgctc tgagcgtcaa gatttatgtg agaggcagtt ttggaaccgg
     5341 tgcgcgagga gacagaagtg gcagcacggc tcagaccgcg ccagccgccg caccgcgctc
     5401 ccgctccgct caactgtggc gcatctgccg cactcgcggc gggcgagtgt agttgtcgcg
     5461 cgacaaagga tgcccgcttc gaccggttgt gtgcgctgtt tgcagggagg aatggagttt
     5521 gcacacagcg gcatgagcgt tgccactgtt tacggctgtt tcaccgttgc agatgatgcg
     5581 cgccccgacc ttaagcgcgt taaagtcgaa aggatcaatc ttctggaaac cttgatcaaa
     5641 cagaccacgc ttgccgcgcc cgcagagacg agcgggatgg gattgcacac gggcgttcgc
     5701 gtacaggtgc gcctgctccc cgccccgccc gacaccggct acatcttcct gcgcacagac
     5761 ctcgacaact tcgagattcc cgccaccgtc gaacacgtcg cccattcgag ttatgcgacg
     5821 actttgatgc gcaagggcgt gacgctctcg acggtcgaac atctgctcgc agccctgcgc
     5881 ggcggcggcg tggacaacgc ctacatcgaa gtggacaacc tcgaactccc gatcatggac
     5941 ggctccgcgg aggtcttcac cgagttgatc gaacgcgtcg gcatcgtcga gcaaccggcg
     6001 acgcggctcg cgctgcgcgt ctgtaaaaaa gtctccgtgg cggaaggcaa acgccggatg
     6061 agcgtcgaac cctcggatgg cttcgagatc gattgcatga tcgacttccc gcacccgctc
     6121 atcggcactc agcgcatgcg cttcgacgcg cttgaggcgt cggcgttcgg gcgcgagatc
     6181 gccagcgcgc gcaccttcgg cttcacggac gagatcgaag ccctgcgccg cgccaacctg
     6241 attcgcggcg gttcgattga caacgccatc gtgctcacgc ccgaagggat gctgaacgag
     6301 acgggcttgc gctcgcgcga cgagttcgtc cgccacaaga ttctcgacat catcggcgac
     6361 ctcgcgctcg tcggcatgcc cctgctcgga ctcgtgcgcg cagagcgcag cggccatctc
     6421 ttccacgcca tgttgatgac aaaactgctg cgcgacgata cggcatggga agtggttgac
     6481 atgcccgtcg gcgcagtggt gcacgcgcat ggcgattgaa gcgcgcggcg cgtccggcgc
     6541 gtcaggtggc aatggttgac cggcttgctt tgttcgtcgt aaaacttgtc tcgcgcccca
     6601 agcagcgact tccccggcac agttcccccg gaaagctcct gaacttatgt caatgaatgt
     6661 cccacagacg gaggccgctg tgtcaaccgc caaaacggag aagttgcgcc gcttctctct
     6721 cgacgcgctc acgcagacgc tcgcgtcgct gaagaacgcg cccacgcaga gcgaactcga
     6781 cgggctgttg cgcatgctgg aaccggagcc ggaagagttg cgcgaacacc tcggcttcaa
     6841 gccgaacacc tacacgcgcc atcgcctctt tcgcaacgac ctctgcgaga tgctcgtcct
     6901 ctgctggctg cccggccagt gcacctccat acacgatcac aacggctctt acggcgcgat
     6961 ccgcatttgt gaaggcgcga tggaggaaga gatcttcgcg ctgaacgagg cgggcgaagt
     7021 cgtgtatgag acaacccaca ggcgcgccgc cggagaggtg acgggcacgg gcgtgccgga
     7081 cattcatcgt atcggcaacg cggagggcag cacggaacgg atggtcacga ttcacgtcta
     7141 cgcgccgccg ctgcgcgtca tcaacaccta tcgtctcggc agcgcggaag tcggcgagtg
     7201 ctggccggac gacccggcaa accccgccaa cggttagaag cacaaaacaa ctgtacacac
     7261 gcgcagacac gcaggcgcaa agtttaatca tgcagcagac ttgatgatta aactttgcgc
     7321 ctgcgtgtct gcgtagttaa acgcttcatg cttgcggcgg cgggggttat tcctcgcgca
     7381 cgaattcata gacaatccgc aggcggagtt cgttcggcag aacttcgtgg cgcacctctc
     7441 tggcgcgcag gcgcaaggag gcgccgctcc ccgtcttcgt catcggcaga cgcgcggaga
     7501 gttgttcggc gcgcagaatc tctatggggt tgacgcgtgc gtcgagcgcg tcctgcacga
     7561 gtccggtgat cccttcgccg agcagcgcgg ggatcttttt caggtgcacc gctttgactt
     7621 cgacgcgcgc cgtcaaagcc tgcttcgctt gatcgaaact gagattgagg ttcgtctccg
     7681 cccagccttc aaatttgaga cagccgatga gcggcgcgct gtaagaaccg gagaaggcga
     7741 cgggagcatg gatgcgtccc gcctgaaaac gcaccgccgt gcgcgtgccg cccatctctc
     7801 gctgcaaggc gatctcgctc gcgcaacccc ggctgccgcc ttcggacgag ttgccgcgcg
     7861 ccagcggaaa ggagggcggg ttcggcagcg tgaacatggc ttcgatgagc gcgttgaaga
     7921 tctgttcgtt cagccccacg acgacttcgc ccgcctgaat cgctttcgtc tcacgcgggc
     7981 gcgcactcgc ctgcgctgca cttacgttgc cgtagcgcgc gatgccgtag cacggcagcg
     8041 cacccgcgta gagactgagc gcgcaaagaa gcgagacggc aaaccgagac agagtttgcg
     8101 agttgaatcc ggcgcgcgcc gggcgcgcag atgcaagact cgcaagcgac gcacgcatct
     8161 ctcttctctt attcatcctt catccctcat ccctcatcct tcatcccttc cgccatccct
     8221 catccttcat cccttcttcc atccctcaca gcccgctgcg gatgatgtcg tgcagccgca
     8281 acagcccgac gcacgttccc gcttcgtcca cgacgggcag gacggagatt tgcgaagggc
     8341 ggtcttccat cagttgcagc gcggcgtagg ccagtgtgtc gggcgaagtc ttgatggggc
     8401 gcggcgtcat cacgtcgcgg ctggtgagcg tttcgagatc ggcgaggcgg ctgcgcccta
     8461 aggcgcggcg caggtcgccg tccgtgatga tgcctgcgag ttgcccttcg ccttcgagga
     8521 cattgaccgc gcccagcccg ccgcgactga tcgcctgcac gacttcgagc agcggcgcgt
     8581 ccgaggcaat cgtcggattc tccgcgccgc cgtgcatcac atcgcccact ttgagcatca
     8641 gccgcttgcc gagacgcccc gccggatggt tgagcgcgaa atcttccggc gtcagtttct
     8701 tgcaatccat caaggtcagg gcgagcgcgt cgccgatggc gagggcgacg gtggtgctcg
     8761 tcgtgggcgc gagattgagc gggcaggctt ccttgtcaac cgaggcgtcg agcactacgt
     8821 ctgcgcgcgc ggcgagcgtc gagcgcaggt tgccgacgac ggcgatgata gccgtctgcc
     8881 tgtgcttgag aaacggcagc atcgccagta gttcgtcggt ttcgccgctg ttgctgatca
     8941 tgatcaggat gtctgcgggc gcgacgatgc cgagtccgcc gtgcaatgcg tcggacgggt
     9001 gcaggaagac tgcgagcgtg ccggtgctcg tcaaagtggc ggcgatcttc tgcgcgacgt
     9061 tgcccgactt gccgacgccg accacgatca ccttgcccgc acaagaggcc agcaattcga
     9121 ccgcccgctc gacctccgca cgcgcaatgc gcgcggcggt ttcggcgatg gcctcggcct
     9181 ccagtttgag aaagcgtgtg aattccgtgt agtgtctctg ttcagccatg cgaaactttc
     9241 gaccgtcgct ttagttcgcg cgcgcgagtt tcgagcatac gacgcgcaaa agtaaaaagg
     9301 caaagggcag aagttagagg ccggagccgg agtttaacaa gagtcggttc ttactgtttc
     9361 aactgttctc taacctctca tctctccccc tgcccgtgag gcatgagtgt tgccgcgcgt
     9421 cgtgtgtgag cgcggagaag ttttaataac gcacgtatcc cgtacaaaac cggaggagca
     9481 agcatcatgg aagaacagga cgaacgacac ggcgaattgc cgaggcagca ggcgggcggg
     9541 gagacgccct cttcggacac cggcgacata catcgcccgc gccccgccgt cgaaagaaag
     9601 accgaagacg gcacggcggg acagggactc ggacaggttt ccggcgcgac cagagaagac
     9661 gcgaaagaaa agggatgagg gatgagggat gagggatgaa tcagaacaca gaagtcagcc
     9721 ggaagacccg cgcgggttgt tttctgactt ct
//
LOCUS       DASWNS010000574         9997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_125886, whole genome shotgun sequence.
ACCESSION   DASWNS010000574 DASWNS010000000
VERSION     DASWNS010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9997
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125886"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1455)
                     /locus_tag="VGB76_19835"
     CDS             complement(67..1455)
                     /locus_tag="VGB76_19835"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0101185.1"
                     /translation="MDRKDIRILVVDDEAMMADSLKQNLAEEGYTVDTALTGADAIEL
                     FDRGGHHVAVCDVQLPDMDGLEVLRHIKDAQPATEVIVMTGYGTVQRAIEATKAGAFW
                     FLEKPFEFEVLLPLIERAVQHRELIVETETMRRNLSTRTEYFNIIGASRQMQTIYETI
                     ESVAKSDANVLIVGESGTGKELIANAIHYNSLRAKKPFIKVNCAALPKELIESELFGH
                     TKGAFTGAHADKDGLIQHAAGGSLLLDEIAEMPVELQPKLLRVLQERSYRKIGSERTY
                     AVDFRLISSTNRPPADAIRDGLLRDDLFYRISTITIHVPPLRERTEDIQLLAEHFFKM
                     YARKYQRPITGISQAAYQRLFAHGWPGNVRELQNVLERAVLLAKSNKIEPVDLPFENG
                     SVPERTAQGAAWDVPSNLTLEEIEKLVIEKTLQRTGGNKQAAANLLGIYRPRLYSKIR
                     KYNIDIGALVSP"
     gene            complement(1551..2240)
                     /locus_tag="VGB76_19840"
     CDS             complement(1551..2240)
                     /locus_tag="VGB76_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101186.1"
                     /translation="MKPIKATINDTDALIELGRASVQIVHDLKNQINGLKLYATFLRK
                     RMERSERPPDELETINKIMAGLERAALDMTTLVRLGRPLELQRASRLDLAVLLAQATE
                     GDFAAPPAPVAEDNGDYRGNFDAPALTEALKNIHAGARSLAARDTVPAVNLRREETEA
                     EGAATTTTPVALIEWQGIKAGNDNDVFSSFNGGTGLRLALAARIIQAHDGTVEHGSDT
                     LRVRLPLRSDE"
     gene            2454..2840
                     /locus_tag="VGB76_19845"
     CDS             2454..2840
                     /locus_tag="VGB76_19845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0101187.1"
                     /translation="MEENGNSKLTVLVVEDFADNRFMMRKLLEMSGYRVVEAVDGKEA
                     VEVAERARPDLILMDLSLPRLDGLDATRRIRELDGLGRVPIVAVSAHDTNDFHADALA
                     AGCNEYVTKPIDFDELDALVKKLLAK"
     gene            2925..3443
                     /locus_tag="VGB76_19850"
     CDS             2925..3443
                     /locus_tag="VGB76_19850"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HEY0101188.1"
                     /translation="MSERYAALLRQHEPALVRTFVDVVYTDRRTDLPALLSYHQLIDY
                     LPDIFDELAGVLDSAALDSEIAEAVRSLRMHSQARFQQGCLIDEVARELIILRDVLND
                     FLWREGRRDSATAEGEIRELRDCLRRTHLFVDEMIAQAVLIYATSLRPQVRTRTSHWP
                     PPRRRRTDFPEQ"
     gene            3454..5889
                     /locus_tag="VGB76_19855"
     CDS             3454..5889
                     /locus_tag="VGB76_19855"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0101189.1"
                     /translation="MRHGKNSRNPLDVRTKLLVMLIVLSLPMLIISLFQLESYQENLT
                     EQAATIARIKAVAAEGALDAWLENRPATIAQGASLAPSAASELYARLRQHASPNADAA
                     LLVFDARGQLVAEPSAPSISHLPSHSAADVHQEKWSDDVERLTSERRSTRYGWSVVVG
                     VPLAEHTPAGRGFLALTAAWALGLMASSFLSIWAVGRFTTPLRRLAASASTLGQGQLH
                     ERFEVETDDEVGTLAAGFNVMAASLETKFNELRTQGAFIEEVLDSLPLGVAVLDSGLI
                     VRKANTTFARIVGRETGSLAGRGVYEAAAGLAVLSEVVEDVRRTRRAFVNYGLPLELV
                     ARTTTGDARNDREEGTKYWDVILWPMSERSEERGDLLLILSEVSKRVRAEKLATAAFA
                     AEKSRAAELESVINQMDEGVIIVDARGRYRVNPAAARIIGRKPGEFRDGVDALIADMA
                     LRDVNGNVMPAEETPLGRALRRGEHVSGEHVQIMRGATAEEGVLAVSATPLIGDGGGR
                     EGVVAVFRDITQEVRQYKELMGAYGRLREHDRLKSAFVANVSHELRTPLNVIIGLCQL
                     LERDRRQPLAPLQSEAVERMGRNARSLLELVNDLLDYSRLEAGRSALHLEEVDVAAVT
                     QDVIKGFAVEAVEKRVRLEADVSPTLGPVSTDRHKLSQVITNLVSNAVKFTATGTIRV
                     TAAPLDETHWFLEVSDTGIGISPEALSFIFDEFRQVDDRLTRPYSGVGLGLAITRKIV
                     ELLEGEITVESRPNEGSHFRIVWPFSAQPRTGTGSLIQDAATTAAAVASGTELPAATD
                     LRRERQNLRTRTG"
     gene            6033..6548
                     /locus_tag="VGB76_19860"
     CDS             6033..6548
                     /locus_tag="VGB76_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101190.1"
                     /translation="MSSVNTNNDDLDASRRKIFIVAGVVSALLIAALVFWATRSNPNS
                     GSQQPQLAGAIRADSPEFAQIRERLVVDFNPDDHAVESTRAIGDVVINMTPKVRNFTG
                     RTVNGLELQATVVDLGGNAIQQRTVIAIPNRQPELEPNKVIEVPIRMEGFKKEDVRAN
                     IRVELTGVKFK"
     gene            complement(6575..7168)
                     /locus_tag="VGB76_19865"
     CDS             complement(6575..7168)
                     /locus_tag="VGB76_19865"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEY0101191.1"
                     /translation="MEAITVNFKLSEKEFIEASHLIVSPKATHRLARAATSCVFMVLG
                     TSLLLSAFDVEFLSTLLIVGSLLPLLIYLYFFQFDVFARRCYRGDIRFRYGMTVTFTD
                     EHIEVQSKLAESKHSWKLYTDVLEGESCYALIYGKEIRMAAMIPKRAFKSKKQHMAFR
                     ELVGAQFARALPVQQVGEIEAAEQEYQPASLEPPDWR"
     gene            complement(7248..8183)
                     /locus_tag="VGB76_19870"
     CDS             complement(7248..8183)
                     /locus_tag="VGB76_19870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0101192.1"
                     /translation="MAAAIEIDDLSKDYEVGFWRKRKVRALDGLSLAVEEGEIFGFLG
                     ANGAGKSTTMKLLMRLIYPTAGRARILGRDIEEISMHASIGYLPEHPYFYDYLTAREL
                     LEYCAELFGYTRRDCRTRAAEMLALVRLEEKSWDKQLRKFSKGMLQRVGLAQALVNQP
                     SIVFLDEPMSGLDPVGRREVRDLIASLRTTGTTVFFCSHILADIEVLCDRAAILQRGR
                     LAHIGRLADLRLAASHTRAMELIVAGANLSQLEPELKKLDGARLTRTPGGARLEVAGE
                     QDVDAALRAARASGATLVSVQPVGNPLEELFTSDN"
     gene            complement(8249..9232)
                     /locus_tag="VGB76_19875"
     CDS             complement(8249..9232)
                     /locus_tag="VGB76_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101193.1"
                     /translation="MTGHTETGATTTGRAARKDAGGRTRIRRREFALALIIVCGLGVS
                     AGLSRWLEARRPRPEQLAQASEELYLKPEQARRLSLGFNAIAADWYWMRSLQYVGRKI
                     GAHDGAIQIDDLSALNLKILAPLLDNATTLDPQFLAAYQYGAIVLPAVDVEAAIKLVK
                     KGIAANPQAWRLHSYLGYIYWQQNRFPEASEAYAAGARIEGAPSWLAAMSAQMATKGG
                     SRETARAIYQNMYQQTDDEQTKRLALSRLLQLQSLDEMDALRRTVLDPHRTRTGNCPQ
                     AWREVAPLLRAARVPLDSYGAPLDPSGVAYVIKPEPCEVELGEKSEIIRKY"
     gene            complement(9229..>9997)
                     /locus_tag="VGB76_19880"
     CDS             complement(9229..>9997)
                     /locus_tag="VGB76_19880"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0101194.1"
                     /translation="VRSIARNAFREAVRDRVLYNLVFFVLLLTGGAIFIGDLSAAQEA
                     KIIVDMSLSAMLLFGVFIAIFVGVGLVYKELERRTVYAIFAKPVGRGEFLVGKYLGLC
                     LTLAVNVLVMGAGVSLALVYVRGGYDPLVVTIWPAIILIFVELMIVVAVALLFSSFSS
                     PALSALLTFAVFIIGHFSADMRLLAGSNGTASAKIFFGTLYYLLPNLSHYTVITEAGH
                     GLTPAPAAFGVALAYAFVYIAVLLAASILIFNRRNFK"
BASE COUNT         1909 a         2956 c         3204 g         1928 t
ORIGIN      
        1 cagagagcgc ggaaacgaca agtttccgcg ctctctgcct ttgaccctga cgctctacct
       61 tttcgcttac ggcgagacaa gcgcgccgat gtctatgttg tatttcctga tcttcgagta
      121 gaggcgcggg cgatagatgc cgagcagatt ggccgccgcc tgcttgttgc cgccggtgcg
      181 ttggagcgtc ttttcgatga ccagtttttc gatctcttcg agcgtgaggt tggagggcac
      241 gtcccacgcc gcgccctgcg ccgtgcgttc aggcacgctg ccgttctcga agggcaggtc
      301 aaccggctcg atcttgttgg acttggctaa gagcacggcg cgttcgagca cgttctgcaa
      361 ctcgcgcacg ttgcccggcc agccatgcgc gaagagtctc tggtaggccg cctgcgagat
      421 gcccgtgatc gggcgttgat acttgcgcgc gtacattttg aagaagtgct cggcgagcag
      481 ttggatgtct tcggtgcgtt cacgaagcgg cggcacgtgg atcgtgatgg tcgagatgcg
      541 atagaagagg tcgtcgcgca ggaggccgtc gcggatggcg tcggcgggcg ggcggttggt
      601 cgaagagatg aggcggaagt ctaccgcgta ggtgcgctcc gaaccgatct tacgataact
      661 gcgctcttgc aggacgcgca ggagcttcgg ttggagttcg acgggcatct cggcgatttc
      721 gtctaagagg agcgagccgc ccgccgcgtg ctggatgagt ccgtccttat ctgcgtgcgc
      781 gccggtgaac gcgcctttcg tgtggccgaa gagttcggat tcgatcagtt ccttcgggag
      841 cgcggcgcag ttcactttga tgaaaggctt tttggcgcgc agagaattgt agtgaatggc
      901 gttggcgatc agttctttgc ccgtgcccga ttcgccgacg atgaggacgt tcgcgtcaga
      961 cttggcgacc gattcaatcg tctcgtagat cgtctgcatc tggcgcgacg cgccgatgat
     1021 gttgaagtac tccgtacgcg tcgagaggtt gcggcgcatc gtctcggttt ccacgatcag
     1081 ttcgcggtgc tggacggcgc gctcgatgag tggcaggagc acttcgaact cgaaaggttt
     1141 ttcgagaaac cagaacgcgc ccgccttcgt cgcttcgatg gcgcgctgca ccgtgccgta
     1201 gccggtcatc acgatcactt ccgtggcggg ctgcgcgtcc ttgatgtggc gcaagacttc
     1261 gaggccgtcc atgtccggca gttgcacgtc gcagacggcg acgtgatgcc cgccgcgatc
     1321 aaatagctcg atggcgtccg cacccgtcag cgcggtgtcc accgtgtaac cctcctcggc
     1381 caaattctgc ttgagcgaat cggccatcat cgcctcgtcg tcaaccacca aaatgcgtat
     1441 gtctttacga tccaccttta ctctcctaga tgaaaataag tgatgagtga tgagtgatga
     1501 gtgatgagtt aaaacagtag acttgtcttt aactcatcac tcattactca tcactcatca
     1561 cttctgagtg gtagccggac gcgcaaagtg tccgagccat gttcgactgt gccgtcgtgt
     1621 gcctgaatga tccgggcggc gagtgcgagg cgcagacccg tgccgccatt aaaactgctg
     1681 aaaacgtcgt tatcattgcc cgccttgatt ccctgccact cgatgagggc gaccggagtt
     1741 gtcgttgtcg ccgcgccctc cgcctccgtc tcttcgcggc gcaggttgac cgccgggacg
     1801 gtgtcgcgcg cggcgagcga acgcgccccg gcatgtatgt tttttagtgc ctcggtgagc
     1861 gcgggcgcgt cgaagttgcc tcgatagtcg ccgttatctt ccgccaccgg agcgggcggg
     1921 gcggcgaagt cgccctcggt ggcctgcgcg agcaggacgg cgaggtcgag ccgggaggcg
     1981 cgttggagtt cgagcgggcg accgagacgc acgagcgtcg tcatgtcgag cgcggcgcgt
     2041 tcgagtccgg ccatgatctt gttgatggtt tcgagttcgt ccggggggcg ttcgctgcgc
     2101 tccatgcgct tgcgcaaaaa cgtggcatag agtttgaggc cattaatctg atttttcaga
     2161 tcgtggacga tctgcacgct ggcgcgcccc aactcgatga gtgcgtccgt gtcgttaata
     2221 gtcgctttta tcggcttcat tttgctcaca aactcgcgcg tcgggagagt ttgccagttt
     2281 cgtagaggac gttaggccgc cttctctatg cgaattgaac ccgaacgatg cggctttttg
     2341 tgttgcgttt cgatgcggca tcttaacaaa aaatccgagt gtttgctagt gatacgtttg
     2401 gcgttggccg caagacgtgg aattatttgc cctcgttgac aggaagggtg tgaatggaag
     2461 agaacggaaa ctcgaagttg actgtcctcg tagtggaaga tttcgccgat aaccgcttta
     2521 tgatgagaaa gttgctggag atgagcggtt accgggtggt ggaagctgtg gacggcaaag
     2581 aggccgtgga ggtggccgag cgagcgcgcc ccgatttgat cctgatggac ttgagcctcc
     2641 cgcgcctcga cggcctcgac gctacgcgcc gcatacgcga actcgacggg ctggggcgcg
     2701 tccccatcgt cgccgtctcc gcgcacgaca ctaacgattt tcatgccgac gcgctcgccg
     2761 caggctgtaa cgaatacgtc accaagccga ttgatttcga cgagttggac gcacttgtga
     2821 agaaactcct agcaaaatga tgaagtagga acgcggaacg atgaagtaaa agcctctgtc
     2881 tttcattccg cgttccgcgt tcctgcctcg tcgtcaagtc tcctatgtcc gaacgctacg
     2941 ccgccctgct ccggcagcat gagcctgcgc tggtgcgcac cttcgttgac gtggtttaca
     3001 cagaccgccg gacagacctg cccgcgctgc tctcttatca ccaactcata gactacctgc
     3061 cggacatttt cgacgaactc gcgggggtgt tggatagcgc tgcgctcgac agcgagatcg
     3121 cggaagcggt gcgttccctt cgcatgcact cgcaggcgcg ttttcagcag ggctgtctga
     3181 ttgacgaagt ggcacgggag ttgataattc tacgcgatgt attgaatgat tttctgtggc
     3241 gcgagggtcg aagagactcc gccaccgcag agggagagat tcgggaactg cgcgattgtc
     3301 tgcggcgaac gcacctgttc gtcgatgaga tgatcgcgca ggcggttttg atctacgcga
     3361 cgagtttgcg tccacaggtg cggacacgca cctcccactg gccgccgccg cgacgccgcc
     3421 gtacggattt cccggaacaa taaaccagcc gtgatgagac atggcaaaaa ctctcgcaac
     3481 ccgctcgacg tgcgcaccaa gctcttagtg atgctcatcg tgctgtctct gcccatgctc
     3541 atcatcagcc ttttccagct tgagagttat caggaaaatc taaccgaaca ggccgccacc
     3601 atcgcacgca tcaaggccgt cgccgccgaa ggcgcgctcg acgcgtggct cgaaaatcgt
     3661 cccgcgacca tcgctcaggg cgcgtcgctc gccccctctg cggcctccga actctacgcg
     3721 cgcctgcgtc aacacgcttc gcccaacgcc gacgccgccc tgctcgtctt cgacgccaga
     3781 ggccaactcg tagccgaacc ctccgcgccc tcgatttcgc atctgccgtc acactcggct
     3841 gccgacgtgc atcaggagaa gtggagcgac gacgtggaac gtctgacgag cgagcgacgg
     3901 agcacgcgct acggttggag cgtggtggtc ggcgtgccgc tcgcggagca cactccggca
     3961 ggtcgcggct tcctcgcgct cacggcggct tgggcgctcg gtctgatggc ttcgagtttt
     4021 ctctcgatct gggcggtcgg gcgtttcacc acgcccctgc gccgcctcgc cgcctcggct
     4081 tcgacgctcg gacagggaca actccacgaa cgtttcgagg ttgagacgga cgacgaagtg
     4141 ggcacgctcg cggcgggctt caacgtgatg gctgcgagtt tggagacgaa gttcaacgaa
     4201 ctgcgcacgc agggcgcgtt catcgaagag gtgctcgaca gcctgccgct cggcgtcgcc
     4261 gtgctcgact ccggcttgat cgtacgcaag gccaacacga ctttcgcgcg catcgtcggg
     4321 cgcgagacgg ggtcgctggc agggcgcggc gtctacgaag cggcggcggg gctggcggtt
     4381 ttgagcgaag tggtcgaaga cgtgcgccgc acgcgccgcg ccttcgtcaa ctacggcctg
     4441 ccgctcgaac tcgtcgcgcg aacgacgacg ggcgacgcgc gcaacgaccg cgaagaaggc
     4501 acgaaatact gggacgtgat cctctggccg atgagcgagc gcagcgaaga gcgcggcgac
     4561 ctgctcctga tactttcgga agtctcgaag cgcgtgcgcg cagagaagtt ggcgaccgcc
     4621 gcctttgcag cggagaagtc gcgcgcggcg gaactcgaaa gcgtcatcaa ccagatggac
     4681 gaaggcgtga tcatcgtgga cgcgcgcggt cgttaccgcg tcaaccctgc ggcggcgcgc
     4741 atcatcgggc gcaagcccgg cgagtttcgc gacggcgtgg acgcgctcat cgcagacatg
     4801 gccttgcgcg acgtgaacgg caacgtcatg cccgccgaag agacgccgct cggacgcgcg
     4861 ctcaggcgcg gcgaacacgt ctccggcgag cacgtccaga tcatgcgcgg cgcgacggcg
     4921 gaagagggcg tgctggccgt cagcgcgacg cccctcatcg gcgacggcgg cgggcgcgaa
     4981 ggcgtcgtcg cggtcttccg cgacatcacg caggaggtca ggcagtacaa agagttgatg
     5041 ggcgcatacg gtcgcctgcg tgaacacgac cgcttgaagt cggccttcgt cgccaacgtc
     5101 tcgcacgaac tgcgcacgcc tctgaacgtc atcatcgggc tgtgccagtt gttggagcgc
     5161 gaccgcaggc agccgctcgc gcctttgcag tccgaagccg tcgaacgcat ggggcgcaac
     5221 gcgcgctcgc tgctcgaact cgtcaacgac ctgctcgatt attcgcgtct ggaggcgggg
     5281 cgttccgcgc tgcatctgga agaggtggac gtggccgccg tgacgcagga tgtcatcaag
     5341 ggtttcgccg tcgaggcggt ggagaagcgc gtccggctcg aagcggatgt ctcgcccacg
     5401 ctcggccccg tgagcacaga ccggcacaaa ctctcgcaag tgatcacgaa tctcgtgagc
     5461 aacgccgtga agtttacggc gacgggcacg atccgcgtga ccgccgcgcc gctcgacgag
     5521 acgcactggt ttctggaagt gagcgacacg ggcatcggca tctcgcccga ggctctgagt
     5581 tttatcttcg acgagttccg gcaggttgac gaccggctga cgcgtccgta tagcggcgtc
     5641 ggtctggggc tggcgatcac gcgcaagatc gtcgaactgc tcgaaggcga gatcactgtg
     5701 gagagccgcc ccaacgaggg cagccacttc cgcatcgtct ggccgttcag cgcgcaaccg
     5761 cgcacaggca ccggctcgct cattcaggac gccgcgacga ccgccgccgc tgtcgcctcc
     5821 ggcacggaac tccccgccgc gacagacctt cgccgcgaac gccaaaactt gcgcacacgc
     5881 acgggatgat aaaacagtga cgagtaataa gtgatgagtg atgagtgatg agtgatgagt
     5941 gatgggtaat aagtggcggg tgacaagtga agggtgacga gataaaattc ctgttgttct
     6001 aactcgtcac ttgtcactcg tcacttaaag atatgtctag tgtaaatacg aacaacgacg
     6061 accttgatgc ttcgcggcga aaaatcttta tcgtcgcggg ggtagtgagc gcgctcttga
     6121 tcgcggcgct ggtcttctgg gcgacgcgct cgaacccgaa cagcgggagc caacagccgc
     6181 aactcgcggg cgcgatccgc gccgactcgc cagagtttgc gcagatacgt gagcgtctcg
     6241 tcgtggattt caacccggac gaccacgcag tggaatcaac gcgcgccatc ggcgacgttg
     6301 ttatcaacat gacgccgaag gtgcgcaact ttaccgggcg caccgtgaac ggtctggaat
     6361 tgcaggccac cgtcgtcgat ctcggaggaa acgcgattca gcagcgcaca gtgatcgcca
     6421 ttcccaaccg ccagcccgaa ctcgaaccga acaaggtgat tgaagtgccg atccgtatgg
     6481 agggcttcaa gaaagaggac gtgcgcgcca acatccgcgt cgaactgacg ggcgtgaagt
     6541 ttaaataagc gcgggcggga agcggaaggg gtctttatcg ccagtcgggc ggctcaaggc
     6601 tcgcgggctg gtattcctgc tccgccgctt cgatctcgcc gacctgctgc accggcagcg
     6661 cacgggcaaa ctgcgcgccg accagttcgc ggaaagccat gtgctgtttc ttgctcttga
     6721 aggcgcgctt cgggatcatc gccgccatcc gaatctcctt cccgtagatg agcgcgtagc
     6781 aactctcacc ttccagcacg tccgtatata acttccagct atgtttcgac tcggcgagct
     6841 tcgactgcac ctcgatgtgt tcgtctgtga aggtgacggt catgccgtag cggaacctga
     6901 tgtcgcctct gtagcaacgc cgcgcgaaaa cgtcgaactg aaagaaatag agatagatga
     6961 gcaacggcaa caacgagccg acgatcaaca gcgtggacaa aaattccacg tcgaacgcgc
     7021 tcagcagcaa agaggttccc agaaccataa agacgcagct tgtcgcggcg cgtgcaagcc
     7081 tgtgcgtcgc ctttgggctg acgatcaggt gagaggcttc gatgaactct ttttccgaga
     7141 gtttgaagtt aacggtaatt gcttccataa cacatgacga acggatcaac gcgggcgaag
     7201 atggcgaaag cgcgttgata gataatgcgg attggaatcg gcgcgtgtca attgtcgctc
     7261 gtaaaaagtt cttcgagcgg gttgccgacg ggttggacgg agacgagcgt tgcgccggag
     7321 gcgcgtgcgg cgcggagcgc ggcgtcaacg tcctgttcgc ccgcgacttc cagacgcgcc
     7381 ccgcccggcg tccgggtcag ccgcgcgccg tccagtttct tcaactccgg ttccaactgc
     7441 gacaaatttg cgcccgcgac gatcaattcc atcgcgcgcg tgtggctggc ggccaggcgc
     7501 aggtctgcga ggcgtccgat gtgcgcgagc ctgccgcgct gaagaatggc cgcgcggtcg
     7561 cagagcactt cgatgtcggc gaggatgtgc gagcagaaga agacggtcgt gcccgtcgtg
     7621 cgcagagagg cgatgaggtc gcgcacttcg cggcggccta cagggtcgag gccgctcatc
     7681 ggttcgtcga gaaagacgat agagggttgg ttgaccaaag cctgcgccag cccgacgcgt
     7741 tgcagcatcc ctttcgagaa cttgcgcagt tgcttgtccc aactcttctc ttcgaggcgg
     7801 acgagtgcga gcatctccgc ggcgcgggtg cggcagtcgc ggcgcgtgta gccgaaaagc
     7861 tccgcgcaat attcgagcag ttcgcgcgcg gtcaggtaat catagaagta ggggtgttcg
     7921 gggagatagc caatcgaagc gtgcatggag atctcttcga tgtcgcgccc gaggatgcgc
     7981 gcgcgcccgg cagtcgggta gataagacgc atgagcagtt tcatcgtcgt ggatttgccc
     8041 gcgccgtttg cgccgaggaa gccgaagatt tcgccttctt cgacggcgag cgaaaggccg
     8101 tcgagcgcgc gcaccttgcg cttgcgccag aagccgactt cgtaatcttt cgacagatca
     8161 tcaatctcaa ttgcggctgc catctctcat tctccgtgaa ctgatcaaga caggaatacg
     8221 gttcggcctt ttagtgtctc gcgcccgatc aatacttgcg tatgatctcc gacttctcgc
     8281 cgagttccac ttcgcaaggc tccggtttga tgacgtaggc gacgcccgaa gggtcgagcg
     8341 gtgcgccgta ggaatcgagc gggacgcgtg cggcgcgaag cagcggcgca acttcgcgcc
     8401 acgcctgcgg gcagttgccc gtgcgcgtgc ggtgcgggtc gagcacggtg cggcggagcg
     8461 cgtccatttc gtcgagcgat tggagttgga gcaggcgcga gagggcgagt ctcttcgtct
     8521 gctcgtcgtc ggtctgttga tacatgttct ggtagatggc gcgggcagtt tcgcggctgc
     8581 cgcctttggt cgccatctgc gccgacatcg ccgcgagcca ggagggtgcg ccctcgatac
     8641 gcgcgcccgc cgcgtatgct tcgctcgctt cgggaaaacg attttgttgc cagtagatgt
     8701 aaccgagata actgtggagt cgccacgcct gcgggttggc ggcgatgcct ttcttaacca
     8761 gcttgatcgc ggcttcaacg tcaaccgccg ggaggacaat cgcgccgtac tggtaagcgg
     8821 cgagaaactg cgggtcgagc gtcgtcgcgt tgtctaagag cggcgcgagg attttcaggt
     8881 tgagcgcgct caggtcgtca atctggatcg cgccgtcgtg cgcgccgatc ttgcgaccga
     8941 catattgcag cgaacgcatc cagtaccaat ccgccgctat cgcgttgaac cccagactga
     9001 ggcggcgcgc ctgttccggt ttcaggtaca actcttccga cgcctgcgca agttgctcgg
     9061 gacgcgggcg acgcgcctcc agccagcgcg acagacctgc cgacacgccc agcccgcaga
     9121 cgataatcaa cgccagcgca aactcgcgcc tcctgatacg cgtccggcct cccgcatcct
     9181 ttcgcgcggc gcgtccggtt gttgtggctc ctgtttccgt gtggcctgtc atttgaaatt
     9241 gcgacggtta aagatcagga tcgaggcggc cagcaggacg gcgatgtaga cgaaggcgta
     9301 agcgagcgcg acgccgaaag cggcgggcgc aggcgtgagg ccgtgccccg cctcggtgat
     9361 gaccgtgtag tgggagagat tggggagcag gtagtagagc gtgccgaaga agattttcgc
     9421 gctcgccgtc ccgttcgacc cggccagcag tctcatgtcc gcgctgaagt ggccgatgat
     9481 gaagacggcg aaggtgagca aggccgagag cgcgggcgaa gagaaggaag agaagagcag
     9541 cgcgaccgcg acgacgatca tcaactcgac gaagatcaaa atgatcgcag gccagatcgt
     9601 tacgacgagc ggatcgtagc cgccgcgcac gtaaacaagc gcgagcgaga cgcccgctcc
     9661 catcaccagc acattgacgg cgagcgtcag gcacaggccg aggtatttgc ctacgagaaa
     9721 ctcgccgcgc ccgacgggct tggcgaagat ggcatagacc gtgcgccgct ccaactcctt
     9781 atagaccagc ccgacgccca cgaagatcgc gatgaagacc ccgaagagca gcatcgcgct
     9841 caaagacatg tcaacaatga tcttggcttc ctgcgccgcc gagaggtcgc cgatgaagat
     9901 cgcgccgccg gtgagcagga ggacgaagaa gaccagattg tagagcacgc ggtcgcgcac
     9961 ggcttcacgg aaagcgttac gcgcaatgga acggacg
//
LOCUS       DASWNS010000575         3438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_255355, whole genome shotgun sequence.
ACCESSION   DASWNS010000575 DASWNS010000000
VERSION     DASWNS010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255355"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..914)
                     /locus_tag="VGB76_19885"
     CDS             complement(258..914)
                     /locus_tag="VGB76_19885"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HEY0101195.1"
                     /translation="MPIRQNSFARRAKERQQQQGLKKVIIERDAQPTIDEQLVRLEDD
                     VRKLKVEFDIYFNGSSKRPPYDTKGRVETLIKRLADERQLTYAQRYQYNSLVARYTSF
                     RDLWRRTIQDREEGRGSLRGSAAKKEKDAQPAATGRTTFVCADAHADEPTVKSLYDTL
                     VEAKRQCGERVEDFSFPQFQRMLAQKTETLKERLGCERVQYSVKLEGGRVQFKAKADK
                     "
     gene            1145..2575
                     /gene="selA"
                     /locus_tag="VGB76_19890"
     CDS             1145..2575
                     /gene="selA"
                     /locus_tag="VGB76_19890"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEY0101196.1"
                     /translation="MPATQLLRALPSIDALLRTDAARTLGERTGLQRLTALARSVTDE
                     LRAELQARASAETAGAAPANGDLSREALLAEAVKRLERAVERVESSGLRRVINATGVI
                     LHTNLGRAPLSDAARRAVADEAAGYCTLEYDLETGTRGRRGAGVEELLSELTGAEAAL
                     VVNNCAAAALLILSTLARDGETIISRGELVEIGGDFRVPDVMAQSGTRMVEVGTTNRT
                     KLDDYKNAINLETQLILRVHPSNYRIVGFTAAPALAELTALARDNNLLVYEDAGSGAL
                     DDLGAYRLQDEPVIRESIRQGADIVSFSGDKLLGATQAGLIVGRRALIERCRKNSLYR
                     ALRADKLALAALAATLAAHARGASVAEVPTMRMLAATYAEIEGRALAFLRRATTRLAP
                     DSFKFEIIAGESAIGGGSAPTTHPPTALIAITHTRFSAVALEAKLRQARPPIVARILD
                     ERVVLDLRTVTAGEESELLDALAALS"
     gene            2725..3240
                     /locus_tag="VGB76_19895"
     CDS             2725..3240
                     /locus_tag="VGB76_19895"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY0101197.1"
                     /translation="MKRPNNYLLKLLYVCVAIFLLAALTLQVLSSMGRLPVTLPPGVP
                     MIVSVASLMLALGVLLRRLWLIDRLLARQQPQRATPLFPDHPVREVLQMQEVEQERSQ
                     RISRMSHELRTPITSINGFAELLMGDESVSGEAREFAGIIHTEAQRLSQMINTVLTEV
                     QLAQDTGMSEQ"
BASE COUNT          675 a         1151 c          927 g          685 t
ORIGIN      
        1 gcagggaccc aaaggtccgc aagccgaaaa agtgacgaag gcgtaatctc cccctcatca
       61 gctttcgctt aggaaagcca ctggccgctt ccggtttctc agagccggta agcgttggat
      121 aagcccgaag aactcgaccg aagttccgac aggcaaaaga tcaaaggcgc gctctggaaa
      181 cttccaagga gcgcgccttt gatcttacag agcgacttga tctcgcttaa ccaactctcc
      241 gcctatctgc tccccgctta tttgtcggcc ttcgccttga actgcacgcg cccgccttcc
      301 aacttcaccg agtattgcac gcgctcgcag cccagacgct ctttcaacgt ctcggtcttc
      361 tgcgcgagca tgcgttgaaa ctgcgggaac gaaaaatcct ccacgcgctc gccgcactgc
      421 cgctttgcct cgacgagcgt gtcatacagg ctcttcaccg tcggctcgtc cgcgtgtgcg
      481 tcggcgcata caaaagtcgt gcgcccggtt gcggcgggct gtgcgtcctt ctcttttttc
      541 gccgcgctgc cgcgcaagct accgcgccct tcctctcgat cttgaatcgt gcgccgccac
      601 agatcgcgaa acgaagtata gcgagcgacg agcgaattgt actgatagcg ttgggcgtaa
      661 gtgagttggc gttcgtccgc caggcgtttg atgagcgttt cgacgcgccc cttcgtgtcg
      721 taaggtggac gcttcgagga gccgttgaag taaatatcga attcaacttt caacttgcgc
      781 acgtcgtctt cgagcctgac gagttgctcg tcaatggtcg gttgcgcatc gcgctcgatg
      841 atgacttttt tgagtccctg ttgctgttgg cgctctttcg cccggcgcgc aaaactattt
      901 tgacgaattg gcacggcttt attactcctc acccgcgctc tctggcgtaa gcgcgtccct
      961 ttcaaacata acggttggtc aaaggcagtg tcaacgaaaa tagttttctg ctgttcgcgc
     1021 tcccttatac gttgttcgtc ctcccctgtt tattgctatc attcaccgct ctttaaccct
     1081 aaaatgctca ggtatcgcca gacaacccaa cggacaaacg gccaccgacg acggacaaac
     1141 tcctatgcct gccacacaac ttctccgcgc cctgccttcc attgacgctt tgcttcgcac
     1201 cgacgctgcg cgcacgctcg gcgaacgcac cggcttgcag cgtctaaccg cgctcgcgcg
     1261 tagcgtgacc gatgaactgc gcgcagagtt gcaggcgcgt gcgagcgcgg agactgcggg
     1321 cgccgctccg gctaatggcg atctctcacg cgaagcgttg ttggccgagg cggtcaaacg
     1381 tctggaacgc gccgtcgagc gtgtggaatc gagcgggctg cggcgcgtca tcaacgcgac
     1441 cggcgtgatc ctgcacacca acctcgggcg cgctccgctc tccgacgccg cgcgccgcgc
     1501 cgtcgccgac gaagcggcgg gctactgcac gctcgaatat gatctggaaa caggcacgcg
     1561 cggacggcgc ggggcgggcg tggaagaact gctctcagag ttgaccggag cggaagccgc
     1621 gctcgtcgtc aacaactgcg ctgccgccgc tctgctcatc ctctcgacgc tcgcgcgcga
     1681 cggcgagacg atcatctcac gcggcgaact cgtagagatc ggcggcgact tccgcgtccc
     1741 cgatgtgatg gcgcagtcgg gcacgcgcat ggtcgaagtc ggcacgacca accgcacgaa
     1801 gctcgacgac tacaagaacg ccatcaacct tgagacgcaa cttatcctgc gcgtccaccc
     1861 ttcaaattat cgtatcgtcg gattcaccgc cgcgcccgcg ctcgcagaac tcaccgcgct
     1921 cgcgcgagat aacaatctgc tcgtctacga agacgcaggc tccggcgcgc tcgacgatct
     1981 cggcgcgtac cggctacagg acgaacccgt catacgcgag tccatcagac agggcgcgga
     2041 catcgtctcc ttcagcggcg acaaactgct cggcgcgacg caagccgggc ttatcgtcgg
     2101 acgccgcgcc ctcatcgaac gctgccgcaa aaattcactc tatcgcgctc tgcgcgccga
     2161 caaacttgcg ctcgctgcgc ttgccgccac gctcgccgcc cacgcacgcg gcgcaagcgt
     2221 ggcggaagtg cccaccatgc gcatgctcgc cgccacgtat gcagagatcg agggacgcgc
     2281 actcgccttc ttgcgccgtg caaccacgcg cctcgcgccc gactccttca agttcgagat
     2341 catcgcagga gaatctgcca tcggcggcgg ttccgccccc accacgcacc cgccgaccgc
     2401 cctcatcgcc atcacgcaca cgcgcttttc agccgtcgcg ctcgaagcca aactgcggca
     2461 ggcgcgccct cccatcgtcg cacgcatcct cgacgaacgc gtcgtactcg acttgcgcac
     2521 ggtcactgcc ggagaagagt ccgaactgct cgacgcgctc gccgcgctct cataaataag
     2581 gagcgacgaa aagggcgcgc gttgcggggc gaaaaaactg cttgcctcaa accgccctgc
     2641 caagtcatac tgaatgttgg cctgtgcgcg ctctcgacgg ccattatctt gaacgcccgc
     2701 ctccggctcc tcggaaataa agttttgaaa cgtcccaaca actacctact caagctgctc
     2761 tatgtttgcg tcgcgatctt tctgctcgcg gctttaacgc tgcaagtctt atcgagcatg
     2821 ggcaggcttc cggtgacgct gccgccgggc gttccgatga tcgtctcggt cgcctcgctc
     2881 atgctcgccc tcggcgttct gctgcgtcgc ctctggctca tcgaccgcct gctcgcgcgc
     2941 cagcaaccgc aacgcgccac gccactcttc ccggatcatc ccgtgcgcga agtcctgcaa
     3001 atgcaggagg tcgaacagga gcgcagccaa cgcatctcac gcatgtcgca cgaactgcgc
     3061 acgcccatca cgagcatcaa cggttttgcg gaattgttga tgggcgacga aagcgtctcc
     3121 ggcgaagcac gcgagttcgc cggaatcatc cacaccgaag ctcaacgact ctcccagatg
     3181 atcaacaccg tcctcaccga agtccaactc gcgcaagaca ccgggatgag cgaacagtga
     3241 caagtgacga gtgataggtg acgagtgaca ggttaaaaag ccgtcaaatc gtcaatcgtt
     3301 aatcgtaaaa agtgaatcgt gaatagagaa gagttattcc ctctattcac gattcacttt
     3361 ttacgattca ctctcttgtc ccgtcacctg tcacttgtca cctgtcactg tttattgatc
     3421 cttccccggc aggtgggg
//
LOCUS       DASWNS010000576         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_438328, whole genome shotgun sequence.
ACCESSION   DASWNS010000576 DASWNS010000000
VERSION     DASWNS010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438328"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /gene="nadC"
                     /locus_tag="VGB76_19900"
     CDS             complement(<1..742)
                     /gene="nadC"
                     /locus_tag="VGB76_19900"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY0101198.1"
                     /translation="MKWLDEGALFQQIGAFLSEDLGRGDITTQSTIARNAHARGRFLA
                     KEPLVIAGLEAAEAVFSTLDSQQQLEAFFSDGDEVEAGKVIARTNGFADVLLAGERVA
                     LNLLQRLSGIATTTRAFVQAIEGTNAQIVDTRKTTPGLRMLEKYAVESGGGRNHRFGL
                     DDGVLIKDNHIALAGGVGAAVERARQSVGHLHKIEVEVSSERDLREAITSGADILLLD
                     NLTPEATRPLVELARELSQSVLLEASGGI"
     gene            complement(739..>1642)
                     /locus_tag="VGB76_19905"
     CDS             complement(739..>1642)
                     /locus_tag="VGB76_19905"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY0101199.1"
                     /translation="EGFDENRDSWALHDRWIVSRLNKTARAVGEAIEKYQFHAAVQTL
                     YHFFWDDFCDWYIELSKSEVTATEGSPARAAARSRVLTVLEQALRLLHPFMPFITEEL
                     WQMLPGIGEQQLHPAYAGAKPTIMLAAYPQAQEALIDERAEAEVAAIIELVSRVRNIR
                     SEMNIKPGEAVPVLVGAPDAKLRAVFEAGATQIARLVRASNISIRENMDAPRASARAV
                     LAGGAEVAVPLEGLIDFAQERERLRREEEKLTKEAERLRGQLGNENFVSRAPAEKVTE
                     LRQRMTDIEQRTGALRQSMEAFAE"
BASE COUNT          274 a          567 c          484 g          317 t
ORIGIN      
        1 ggatgccgcc ggaggcttcg agcagcacgc tctgcgacag ttcgcgcgcg agttcgacga
       61 gcgggcgcgt cgcctccggc gtcagattat cgagcagcag aatatccgcg ccgctcgtga
      121 tcgcttcgcg caggtcgcgc tcgcttgaga cttcgacctc gatcttgtgc aggtggccga
      181 ccgactggcg cgcgcgttcg accgccgcgc cgacgccgcc cgccagcgca atgtgattgt
      241 ctttgatgag caccccgtcg tccaaaccga aacggtgatt gcgcccgccg cccgactcca
      301 ccgcgtactt ctcaagcatg cgcaagcccg gcgtggtctt ccgcgtatcc acgatctgcg
      361 cgttcgtccc ttctatcgcc tgcacgaacg cgcgcgtcgt cgtggcgatg ccggagagac
      421 gttgcagcag attcagagcg acgcgttcgc ccgccagcag cacgtctgcg aaaccgttcg
      481 tgcgcgcgat gaccttgccc gcttccacct cgtcgccgtc ggagaagaag gcttcgagtt
      541 gctgctgcga atcgagcgtg gagaagacgg cctccgccgc ttcgagtccg gcaataacga
      601 gcggctcttt ggcgagaaaa cgaccgcgcg cgtgcgcgtt gcgcgcaatg gttgactgcg
      661 tggtgatgtc gccgcgtccc aggtcttcgc tgagaaacgc gccgatctgt tgaaagagcg
      721 caccttcgtc gagccacttc attctgcgaa cgcctccatg ctctgcctga gcgcacctgt
      781 tcgttgctcg atgtccgtca tgcgttgccg caactccgtc accttttcgg cgggcgcacg
      841 ggagacgaaa ttttcgttgc ccaattgccc acgcagacgc tccgcctctt tagtcaactt
      901 ttcctcttcc cggcgcagac gctcgcgctc ctgtgcgaag tcgatcaacc cttcgagcgg
      961 cacggccacc tccgcgccgc ccgccagcac cgcacgcgcc gacgcacgcg gcgcatccat
     1021 gttttccctg atcgaaatgt tcgacgcgcg cacgagccgc gcgatctgcg tcgcgcccgc
     1081 ttcaaacacc gcacgcaact tcgcgtccgg cgcaccgacg agtaccggca cggcctcgcc
     1141 cggcttgatg ttcatctccg aacggatgtt ccgcacgcgc gagacgagtt cgataatcgc
     1201 agccacttcc gcttcggcgc gttcgtcaat caaagcctcc tgcgcctgcg ggtaagccgc
     1261 gagcatgatg gtcggcttcg cgcccgcgta ggccgggtgg agttgttgtt cgccgatgcc
     1321 cggtagcatc tgccagagtt cttccgtgat gaagggcatg aaggggtgga gcaggcgcag
     1381 agcctgttcg agcaccgtca gcacacggct gcgcgccgcc gcgcgtgcgg gcgagccttc
     1441 tgtcgccgtc acctccgact tcgacagttc gatgtaccag tcgcaaaaat catcccagaa
     1501 gaaatgatag agcgtctgca ccgccgcgtg aaactggtac ttctcaatcg cttcgccaac
     1561 cgcgcgcgcc gtcttgttca gacgcgacac gatccagcga tcatgcaacg cccaactgtc
     1621 gcggttttcg tcgaagcctt cg
//
LOCUS       DASWNS010000577         4306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_230863, whole genome shotgun sequence.
ACCESSION   DASWNS010000577 DASWNS010000000
VERSION     DASWNS010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230863"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VGB76_19910"
     CDS             complement(<1..342)
                     /locus_tag="VGB76_19910"
                     /inference="COORDINATES: protein motif:HMM:NF013891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA];
                     GO_process: GO:0002184 - cytoplasmic translational
                     termination [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HEY0101200.1"
                     /translation="MSEKDVIKETRPRMEASVDDLRRRLSSVRTGRAVVTLLDPVVVD
                     YYGTPTPLNQMASIHVPEPQMITVQPWDQSQLNAIEKAIRTADLGLNPSNDGKMVRVP
                     IPALTEERRKQL"
     gene            complement(411..1184)
                     /gene="pyrH"
                     /locus_tag="VGB76_19915"
     CDS             complement(411..1184)
                     /gene="pyrH"
                     /locus_tag="VGB76_19915"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY0101201.1"
                     /translation="MSKVETLMADDQPAAPAFRRILLKISGEALMGAREYGVDVDVAR
                     RVAEEIKAVHDTGVEVAVVVGGGNIFRGVSKSAGNMDRSSADYIGMLATVMNACVLQD
                     ALETTGVHTRILSALNIPEVAEPFIRRRAIRHLEKRRVVIFAGGTGNPYFTTDSAAAL
                     RALEIKAEVILKATKVEGIYNADPMKDATATRYDCITYREVLEQRLQVMDASAISLCM
                     DNGLPIVVFNMRNSGNIMRVVMGEAGVGTKVCMEKPEEQ"
     gene            complement(1291..1956)
                     /gene="tsf"
                     /locus_tag="VGB76_19920"
     CDS             complement(1291..1956)
                     /gene="tsf"
                     /locus_tag="VGB76_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY0101202.1"
                     /translation="MAEITAAGVKSLREKTGAGMMECKNALTEAGGSEEQAIELLRKR
                     GLASAKKKEGRVAAEGAVGSYIHMGGKVGVLVEVNCETDFVARTDEFQSLIRDIAMHV
                     AAAEPRYVTREEVPAGDLDKEREIARAQAKNDPKNANKPDTVIDKIVEGRLNKFYEEA
                     VLLDQPFVKDPAKTVGDLLTEKVSKTGERITVRRFARYKMGEGLAKRDEDFGGEVAAL
                     AGN"
     gene            complement(2102..3199)
                     /gene="rpsB"
                     /locus_tag="VGB76_19925"
     CDS             complement(2102..3199)
                     /gene="rpsB"
                     /locus_tag="VGB76_19925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY0101203.1"
                     /translation="MAVTVTMKELLEAGVHFGHQVRRWNPKMKEYIFGERNGINIIDL
                     QKTQKMFREAIGFVTNMASEDRGRTILFVGTKRQAQDAIREEASRANQYYINQRWLGG
                     LLTNFQTVQKSIKRLRDLETMQTDGRYEMLTKKERIKLDRERETLEKNLSGIKNMTRL
                     PDALFIIDVRKEEIAVQEANRLGIPVIAVVDTNCSPEGIDYVIPGNDDALRAVRLFAS
                     RIADAVVEGQEMRSKAADEDGTAAASQDMGAAGATTGAATDTGTRNSLSASGDGTSSV
                     TTARDPGREAQNYTDMGASAPSTQTQVSVPGNIGFGRTGQQPDSVGDHPVEGTGAPVR
                     GASGGGEDESALPRPDDAHQTKGAESVGVAK"
     gene            complement(3389..3781)
                     /gene="rpsI"
                     /locus_tag="VGB76_19930"
     CDS             complement(3389..3781)
                     /gene="rpsI"
                     /locus_tag="VGB76_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY0101204.1"
                     /translation="MADIQYYGTGRRKTSTARVYLRPGTGEIQVNKKGFDQYFPNQAL
                     RMVIRQPLQLTETVSKFDILVNVDGGGPAGQAGAIRHGITRALMEFNADLRPTLKQAG
                     LVTRDPRIKERKKYGQKGARKRFQFSKR"
     gene            complement(3817..4278)
                     /gene="rplM"
                     /locus_tag="VGB76_19935"
     CDS             complement(3817..4278)
                     /gene="rplM"
                     /locus_tag="VGB76_19935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY0101205.1"
                     /translation="MSTYFPSGQGLAEKRKWYVVDAAGQTVGRLATEVARVLSGKNNP
                     QWTPFMDMGDHVIVINARQAVFKGAKAEQKVYRHHTLYPGGLREISVKDQFARHPERV
                     IELAVRGMLPKNKLGKAMGKKLKVYGDANHRHAAQKPEPLALDPRAGQQQQ"
BASE COUNT          723 a         1332 c         1253 g          998 t
ORIGIN      
        1 cagttgcttg cggcgttctt ccgtcagagc cgggatcggc acgcgcacca tcttgccgtc
       61 gtttgaaggg ttcaggccga ggtcggcggt gcgaatggcc ttttcaatcg cgttgagttg
      121 cgattggtcc cagggctgca cggtgatcat ctgcggctcc gggacgtgga tcgaagccat
      181 ctggttgagc ggcgtcggcg tgccgtaata atcgacgacg accggatcga gcagcgttac
      241 caccgcgcgc cccgtgcgca cgctcgacaa cctgcggcgt aaatcatcaa ccgacgcctc
      301 catgcgcggg cgcgtctctt taatcacatc tttctcgctc atatgttctc cgagtgacga
      361 gtgacaggtt gaagacaagc cttttgcttt aactcgtcac ctgttacttg tcactgttct
      421 tctggtttct ccatgcaaac tttcgtgccg actcccgctt cgcccatcac gacccgcatg
      481 atgttgccgg agttgcgcat attgaagacg acgatgggca acccgttgtc catgcagagc
      541 gagatggccg acgcgtccat cacctgcaag cgttgctcta aaacttctct atacgtgatg
      601 caatcgtagc gcgtagccgt cgcgtccttc atcgggtcgg cgttgtagat gccttcgacc
      661 ttcgtggcct tcaggatcac ttctgccttg atctccagag cgcgcaaggc cgccgccgag
      721 tccgtcgtga aataaggatt gcccgtgccg cccgcgaaga taacgacgcg ccgtttctcc
      781 aggtggcgga tggcgcggcg gcggataaag ggttcggcca cttccgggat gttgagcgcg
      841 gaaaggatgc gcgtgtgcac gcccgtcgtc tccagagcgt cttgcagcac gcaggcgttc
      901 atcacggtgg cgagcatgcc gatgtagtcc gcgctcgaac gatccatgtt gcccgcgctc
      961 ttcgagacac cgcgaaagat gttgccgccg ccgaccacga ccgccacctc gacgcccgtg
     1021 tcgtgcacgg ccttgatctc ttcggccacc ctgcgcgcca cgtcaacgtc aacgccgtac
     1081 tcccgcgcgc ccatcaaagc ctcgccggag attttcagta gaatccggcg aaacgcgggc
     1141 gcggcaggct ggtcgtcggc catcagtgtc tcaactttac tcatcggtgc attcttcgtc
     1201 ggcggaaact tcaatggcga aattcggatt gcggattaga aaacaaaaaa gtctgttttc
     1261 tatcacccgc gatccgaatc tcgccgtgga ttagtttcct gcgagcgcgg ccacttcgcc
     1321 gccgaagtct tcgtcgcgct tggcgagtcc ctcgcccatc ttataacgcg cgaaacgacg
     1381 cacggtaatg cgttcgccgg ttttcgaaac cttctcggtc aacagatcgc cgacggtctt
     1441 ggccgggtct ttgacgaacg gctggtcgag cagcaccgct tcctcgtaga acttgtttaa
     1501 gcggccttcg acgatcttgt cgatcaccgt atcgggcttg ttcgcgttct tcggatcgtt
     1561 tttggcctgc gcgcgtgcga tctcgcgttc cttgtcgagg tcgcccgcag ggacttcctc
     1621 acgcgtcacg tagcgcggtt ctgccgccgc gacgtgcatg gcgatgtctc tgatgagcga
     1681 ttggaattcg tctgtgcgcg caacgaagtc cgtctcgcag ttgacctcga cgagcacgcc
     1741 gaccttgccg cccatgtgta tgtaagagcc gaccgcgccc tctgccgcga cgcgaccctc
     1801 ctttttcttc gccgaagcga ggccgcgctt gcgcagcaac tcgatagcct gttcttcgct
     1861 tccgcccgcc tccgtcagcg cgttcttgca ctccatcatc cccgcgcccg tcttctcgcg
     1921 caacgattta acgcccgctg ctgtgatctc tgccatgatt gctctcctga atagctgtta
     1981 gctgttagct aaaccaaaaa acttccggct aacagcttta ctaaaacaaa gcgcagcccg
     2041 gcggttgtcc gctgttggag gctgcgctcg ccgaaaaatc gaatcttaac ctatggggaa
     2101 attactttgc gacgccgacc gactccgcgc ccttcgtctg atgcgcgtcg tccgggcgag
     2161 gcagtgcgga ctcgtcttca ccgccgccgc tcgctccgcg cacgggcgcg cccgtacctt
     2221 caaccggatg atcgcccacg ctgtcgggct gttgacccgt gcgcccgaag ccgatgttgc
     2281 cgggcacgga cacttgcgtc tgcgtcgaag gcgcgctcgc gcccatgtcc gtgtagttct
     2341 gcgcttcgcg tccgggatcg cgcgccgtcg tcacggacga ggtgccgtcg ccgctcgcgc
     2401 tgagggagtt tctcgtgcct gtatcggtgg ccgctccggt cgtcgcgcct gccgcgccca
     2461 tgtcctgtga ggctgccgcc gtgccgtctt cgtccgcagc tttcgaacgc atctcctgtc
     2521 cttcgaccac cgcatcggcg atgcgcgagg cgaagagacg cacggcgcgc agcgcgtcgt
     2581 cgttgccggg gatcacgtag tcaatccctt cgggcgagca gttcgtgtcc acgacggcga
     2641 tgacggggat gccgaggcgg ttcgcttcct gcacggcgat ctcttccttg cgcacgtcga
     2701 tgataaagag cgcgtcgggc aggcgcgtca tgttcttgat gccggagagg ttcttctcca
     2761 gcgtctcgcg ctcgcggtcg agcttgatgc gctccttctt ggtgagcatc tcgtaacgcc
     2821 cgtccgtctg catcgtttcg aggtcgcgca gacgcttgat ggacttctgc acggtctgga
     2881 agttggtgag cagtccgccg agccagcgtt gattgatgta atactggttg gcgcgcgagg
     2941 cttcttcgcg gatggcgtcc tgcgcctgtc gtttcgtgcc gacgaagagg atggtgcgcc
     3001 cgcgatcttc cgaagccatg ttggtgacga agccgatggc ctcgcgaaac atcttctggg
     3061 tcttttgcag gtcgataatg ttaatgccgt tgcgttcgcc gaaaatgtat tccttcatct
     3121 tcggattcca acgcctgact tggtggccga aatgtacgcc cgcctccaag agttctttca
     3181 tcgtaaccgt gactgccaaa agtgagcctc cttagttgat tggtttaatg tactcgccgt
     3241 gcccgttccc gcgtctcccg acaaagggag agcaaccaac ccggacgacg acaatggact
     3301 aactgcaagt gacgagtgac aagtgacaag tgacgagtaa aagctgtttt cgcttgtcac
     3361 tcgtcacttg tcacctgtca ctgaattctt atcgcttcga gaactggaag cgtttgcgtg
     3421 cgcccttctg cccgtacttc ttgcgttcct tgatgcgcgg gtcgcgtgtc acgagtccgg
     3481 cctgtttcaa ggtggggcgc aggtctgcgt tgaactccat cagggcgcgc gtgatgccgt
     3541 ggcggatcgc tccggcctgt ccggcggggc cgccgccgtc aacgttgacg aggatgtcga
     3601 acttcgacac cgtctcggtc aactgcaagg gctggcgaat caccatgcgc aacgcctgat
     3661 tcggaaaata ctgatcaaag ccttttttgt tgacttgaat ttcgcccgtg ccgggtcgca
     3721 ggtagacgcg cgccgtagag gtcttgcgcc gtcccgttcc gtaatactga atatcagcca
     3781 cgatgtttcg tccttaaaaa tctgaagagg tggaaattac tgttgctgct gcccggcgcg
     3841 cgggtcgagc gcgagcggtt cgggcttttg cgccgcgtga cgatgattgg cgtccccata
     3901 gactttcaac ttcttgccca tcgccttgcc gagtttgttc ttcggaagca ttccgcgcac
     3961 ggcgagttcg atcacgcgtt cggggtgccg ggcaaattga tccttgacgc tgatctcgcg
     4021 caagccgccg gggtagagcg tgtgatggcg atagactttc tgctcggcct tcgcgccttt
     4081 gaagacggcc tggcgggcgt tgatcacgat cacgtggtcg cccatgtcca tgaagggcgt
     4141 ccattgcggg ttgtttttgc ccgaaagcac gcgcgccact tccgtggcga gccgcccgac
     4201 cgtctgcccc gccgcatcga cgacgtacca tttgcgtttt tcagcgagac cctgcccgct
     4261 cggaaagtat gttgacatag ataactctct aggaaaatag cgtctg
//
LOCUS       DASWNS010000578        16505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_22017, whole genome shotgun sequence.
ACCESSION   DASWNS010000578 DASWNS010000000
VERSION     DASWNS010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16505
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22017"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VGB76_19940"
     CDS             <1..606
                     /locus_tag="VGB76_19940"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101206.1"
                     /translation="TRVFYAHLLSNAGRHTEALAEIKRARELDPLSSFAAALEGQFLL
                     HAGRTDEALDRLQKAIDLDPNFYFPHLFAISAYTEKGMYREAIAEAHRAKALAPDQTM
                     SDAYEAYALAKSGKRDEARAVLERLLKLSEQAEKRFVPPYHIALIYNGMDERDEAFAW
                     LERGVVQRDAKMTFLKVEPKWNNLRSDPRFAELMRKVGLPQ"
     gene            complement(689..1099)
                     /locus_tag="VGB76_19945"
     CDS             complement(689..1099)
                     /locus_tag="VGB76_19945"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEY0101207.1"
                     /translation="MEYSDPAIRLTLLPRDTNPQGTIFGGIILSYIDIAGAVEAHRRT
                     QIERFVTVAMREVIFHQPVFVGDLVSFYASTVRMGTTSITIRVVVEAERYGGTSERIK
                     VTEAEVVYVAVDANRRKMRIAENIKEKFNNLTAE"
     gene            1447..3507
                     /locus_tag="VGB76_19950"
     CDS             1447..3507
                     /locus_tag="VGB76_19950"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0101208.1"
                     /translation="MPNSFSRFYAVRALLVAIFAALAFASNVSAQQRGGGTRFDVTNY
                     RIEAELRPAEHLLRANADVTFTPLDTTRSVVFELNGALKVESIERNGKPLANFVQDPA
                     GLESIGPNVRVDLGEVVPANTPVTLRFRWSGALVTAEGGPLVTRRLAYVGNEGSYLMY
                     AARWFPFHDYAADRATADITVIVPNGFQVAGSSDEPPTQSAGAAGTTRYRFVHRQPAL
                     IGNIAAGRYVTRTLRIGGYELQFFVQPGSEGRIERFGEVLGQALQFYTKQYGAPAFGT
                     RYTIAQIDDDSLEAYAAPGMQFLSASFFDPARTTKEERLQREAAYQWWGFTVALKSFD
                     DAWLSQGLAEWSAFALRESSLSGAQLDASQRDMQERALMFEQSASILRAPSTLDDTSP
                     QYQSIVFYKGAMVFRMLRETIGKEKFDALLRTLFEQYRGKSVSIDDFERLASQQVGTN
                     MRYFFARWVEGTGVPEFTVDYQIIRTRTNKFRARGTVKQNFEGLRMPVEVMLRAEGDN
                     QNQITSVRIEEKSEDFDFESNGAPLEVIVDPNNKILRLSEDLRVSVVARRGIELYREG
                     QYAEAQRQLEEALKLDKSNSWVYYNLGLIYLEQRNYDPALDNFQAALDGNLKPSWIEV
                     WAHIKRGNAYDSKGDRARATNEYNKAVETGNNYDNAQKVAKQYLATPYDPRAASQQQQ
                     ASSN"
     gene            complement(3696..4511)
                     /gene="lpxI"
                     /locus_tag="VGB76_19955"
     CDS             complement(3696..4511)
                     /gene="lpxI"
                     /locus_tag="VGB76_19955"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEY0101209.1"
                     /translation="MRYGLIAGNGKFPFLVIEGARRAGVELSVAAIREETDPSIEAAT
                     GAGGRVEWVGIGQLGRMIRFFKREGVERAIMAGQVKHVQIFSGALPDVRMLKLLLRLP
                     RRNTDALIGGIADELRSEGIELIDSTYFLQDHLPATGKLTKREADARERGDIEYGLEV
                     AREIARLDLGQTIVVRARACVAIEAMEGTDACIERAGRLARGRLTVVKVAKPDQDMRF
                     DVPVVGVPTIERMIEAGATCLCITAGKTLMFDREEMLRLADKQRIAIVAVPPV"
     gene            complement(4670..5440)
                     /gene="lpxA"
                     /locus_tag="VGB76_19960"
     CDS             complement(4670..5440)
                     /gene="lpxA"
                     /locus_tag="VGB76_19960"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY0101210.1"
                     /translation="MIHPTAIVSDRAQVSADCFIGPYCVIGDEVVLGKGVRVESHAVI
                     EGRTHIGDETHIYPFASIGLAPQDLKYRGEPAETRIGARNQIREFVTIHRGTEGGGML
                     TAIGDDGLFMAQAHIAHDCLIGNRVIMANAATLAGHVSVADGANIGAYSGVHQFCRIG
                     REAYVGGYSVVVKDALPFALTVGNHAKCYGLNKEGMRRRNYPRETIAALHHAFHLLLN
                     ARLNTTQALARIRAEIKDSVETEELLRFIETSKRGIVK"
     gene            complement(5534..5986)
                     /gene="fabZ"
                     /locus_tag="VGB76_19965"
     CDS             complement(5534..5986)
                     /gene="fabZ"
                     /locus_tag="VGB76_19965"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY0101211.1"
                     /translation="MEIVYDTRAIQELLPHRYPFLLVDRIIELVPKERIVGIKQVTIG
                     EQFFQGHFPGAPVMPGVLIVEALAQVGAILALREIERREEKLVLFSGIERARFRQPVV
                     PGDTLVLEVTALRIGSRVQRMRGEAKVEGRVVAEAEIMSVVAERSGVQ"
     gene            complement(6081..6725)
                     /locus_tag="VGB76_19970"
     CDS             complement(6081..6725)
                     /locus_tag="VGB76_19970"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY0101212.1"
                     /translation="MKLFRFIAAAFVIAASLSLSASAQTPARPQPAAPAGGGTAATGT
                     GRIAVIDSAAFSDDKAGIGRVVNAMKTVDAQFQPLRTEVQGMQTRYEALVADINKTRD
                     VADPKTIATKVDQADTMKRDIERKTQDAQISIQKRMQEVLGPLQEDVYNTLQAFAQAR
                     GISVIIDASRVPLIYFNDTVDITREFIAEYNRAKPATASAAPAGGAAARPAGRP"
     gene            complement(6833..9169)
                     /locus_tag="VGB76_19975"
     CDS             complement(6833..9169)
                     /locus_tag="VGB76_19975"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY0101213.1"
                     /translation="MQGRPGEPRIEGLGRRRTGFPILPLPLLSSTDEALRITIPVIEG
                     KVYRLGDLKIEGNSIFDEKVIRAVIGLKQGDVASGERIYKGLIEDLKKLYGNNGFIQY
                     TAEPEPTYRDNPANPEEGIADYTITIDEGKQFTLRRLEFTGNTFTRDNVLRREFVLNE
                     GDIYNQSYVEYSVLRLNQLGFFDPIDKDADVDFRQNEEEGLVDVNVKVAERGRQQISF
                     NGGLSGIGGSFFGLDYSTNNLLGRGESLSLNFAAGNRQQSFVFSFTEPQIRNRPISAG
                     FSVFVQSLKFFGEGTFLSQNLAALQGLSGSQLDFLNTGDENLFTQRTVGASFFATAPL
                     SEFYKKRPFTLSTRIGLSYSLSQTSVRDPEVNADPSNPQNAIPVIYRQPNILTSRLTP
                     SIVYDSRDVRGVDAIGGKQIAVQLAFAGLGGDVRTYQPSLTYQQFIPVRNKRSNNPHV
                     FGFRLLVGHVGAFATSAKIREAQTTSLSFIQGVPVYERFFLGDEFTIRGYNVRSISPI
                     VPIEQFVTSQNVVLASNPAGAPVPVESANLQNLARQLGTFTGAEGGNVIANPTRGFQP
                     IGADTQLLGNFEYRVPIFGPVSIAGFADIGSAFNLRRGADQIFASSFNNDNSAFPFLA
                     SQLGGFRGSLGALVLEKNPFLAAQITSRGNGLFDVGLLARDNRLVTQEEFAQESCNTC
                     TLDPITGLPFGFQRVFVRGDAQTNTAVRLSQAVFDKIGDFRSSVGLEVRVQLPVVNVP
                     LRLIGFYNPNARRGVNEQVPLFFQEEKIGYRFSIGRTF"
     gene            complement(9170..9973)
                     /locus_tag="VGB76_19980"
     CDS             complement(9170..9973)
                     /locus_tag="VGB76_19980"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY0101214.1"
                     /translation="MRINAPLRRFLLAALIIGQTALLLTAFPADVRAQEPTDAPAAAQ
                     TQKLVESVDIQGNRRNRDEDLLYYVTTRAGDPFNEAQVARDLKALNDLNFFDKTKSRV
                     LTLTGPRGGVEVIFEVEELPIIRELVFDGLSSITESDVLKAFREKRIGIQKENISDPV
                     KLNNARRLIKELLAAKGHPNATVEFEQEVVSATSTSITFKIDEGDRVRVVEVEFEGNR
                     KFKEGELRGAMKYVKEAGLISRFKGQDILDREKLDVDLRLIRNYMASKG"
     gene            complement(10276..12660)
                     /locus_tag="VGB76_19985"
     CDS             complement(10276..12660)
                     /locus_tag="VGB76_19985"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY0101215.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGAPAIEPEHLLLGLMREDKTL
                     TARFLARAQASLEAIRKEIEGRAPLREKISTSVELPLAPETKRVLAYAHEESDRLQHR
                     HIGTEHLLLGLLREERSMAAEILYERGLRLAAVREEVARATGADPRASQKKDTPHLAE
                     FSRDLTEDAANDKLDPLVGRDNEIDRVIQILCRRTKNNPVLIGEPGVGKTAIVEGLAQ
                     RIMRGDVPSFLENKRILSLDLSLIVAGTKYRGQFEERLKQIMRELIENPQYIVFIDEL
                     HTLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPAEFRKSIEKDRSLERRFQAVKV
                     PPPSEEEAVRILDGVRERYEAFHQVRYTEEAIEAAVYQSSRYIPDRFLPDKAIDVIDE
                     AGARVKLRVRREPGLGDNPRAPLSEERLRASYIVDNLTDARITEEDDILVSVSVTKDD
                     VEDVIARWTGIPVNSLKEEEQQKLLRIEIELHGRVVAQRPAISALARSIRRSRAGLKN
                     PQRPVGSFLFLGPTGVGKTEVARSLAEFLFGSERSMIRFDMSEFMEKHSVSKLIGSPP
                     GYVGHEEGGQLTERIRRSPYSVLLFDEIEKAHPDIFNVLLQVFEDGILTDALGNTVDF
                     KNAIIIMTSNIGARFIQKRGALGFQAHAIDSREKLEEMVMSSVKQTFNPEFINRLDEI
                     IIFDELQDEELLEIVGLQVELMNRMLRRRGLEVRLTEEAKQWIVKKTCADRTYGARPL
                     RRALQKYVEDPLSEALIQGELGSATLIEVYEDGGVLGHRPAAGVEELGDELLIH"
     gene            complement(12924..13565)
                     /locus_tag="VGB76_19990"
     CDS             complement(12924..13565)
                     /locus_tag="VGB76_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101216.1"
                     /translation="MNQKIFRGLTLSVSLCALTLAASAQQPARTRRGGLDNLSLPSTS
                     ARVSAATMAGAAWQTFAPEGAGFSVSLPGQPEEPTQSGRESGQLAAQFRSYRLAAGGL
                     KYEVARTPPLPAQLVSQPGFMDKFFASAASNMTRSLLAENPNIKFRLVSEQAISLDGY
                     EGRELEFAAEGHRAVARVFVVEGSVYALGVLGAKSEMTPDKVSRFLDSLALTQ"
     gene            complement(13768..14457)
                     /locus_tag="VGB76_19995"
     CDS             complement(13768..14457)
                     /locus_tag="VGB76_19995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0101217.1"
                     /translation="MRLETCLEVRELRKAYASPSGGAVEVLRGVSFTLPAGEMLAVTG
                     ASGAGKSTLLHLLGGLDAADGGSVRLGEIELTRAVGAELARVRGEQVGFVFQFHHLLP
                     GLSALENVALPLLVARRKPREAREAASVLLAAVGLSERAAHRPGELSGGEQQRTAIAR
                     ALVHAPRLLLCDEPTGNLDARTGESVAALLSTLARTRGAGVIVATHNEQLARTCDRTL
                     LLEDGLLKAQT"
     gene            complement(14549..15907)
                     /locus_tag="VGB76_20000"
     CDS             complement(14549..15907)
                     /locus_tag="VGB76_20000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0101218.1"
                     /translation="MPFELFLALRYLQRSKRRGRQRSARVTALAGVAGIACGVGALIF
                     ALALSNGFQDEMRAKILGGTAHITLTRADGGKLIEDTPAVIARLRTIEGVVDAAPTSY
                     TGALLSGAGGDSYTILRGVEKTSARAVAEIRRTLVEGAIEPLFEETQDAERPDPQLRR
                     YPVTPVPGETEPDTGTLFSTLPDEQDASPAPIVIGAELAARTGLRRIGDEGWLITGQR
                     FSEPPWFVTQTSRVRVVGLFRSGLYEYDATWVYVPLPHTKGEKGAQPGAPVISIETAD
                     IYRTRETAARVRDALGANFKTVDWQEVNAPLFAALQLERRTVALIIALIMFVAALNIT
                     TTLVLVVIERRPDIAILGAMGARPHSIMLIFICEGAIIGAVGACAGVALGLAACFIGE
                     RFKLVSLPADVYSLSAIPFHPHAGDVVLAALFAFIISLLATIYPARAAARLRPAEALR
                     YE"
     gene            complement(16049..>16505)
                     /locus_tag="VGB76_20005"
     CDS             complement(16049..>16505)
                     /locus_tag="VGB76_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101219.1"
                     /translation="TGTLVYIFAARTLASHALTKEYRARLISLPKDAVVMAGGQTVAV
                     NYYRGLGLGQWDVIGTGGGWPGAERLPEQIEWHLTQGHRVLVDTDPRLWSMDSWHEQE
                     TRALVNLETRFRFRRFSETLYEIRPPGDQTARDEPNLRRLLGNPKSLFR"
BASE COUNT         3148 a         4989 c         5178 g         3190 t
ORIGIN      
        1 actcgcgtgt tttacgcgca cttactttcc aacgcgggac gacacacgga agcactggca
       61 gaaataaaac gcgccagaga actcgacccg ctatcctcat tcgccgccgc gctcgaagga
      121 caatttttat tacacgccgg acgcaccgat gaagcgttag acagattgca gaaagcgatt
      181 gacctcgacc cgaatttcta tttccctcac ctatttgcca tcagcgcgta taccgagaaa
      241 ggaatgtacc gggaagccat cgccgaagcg caccgggcga aagcgcttgc acccgaccaa
      301 accatgtcag acgcctacga ggcttacgct ctggcgaagt cgggcaaacg cgatgaggcg
      361 cgcgcggtgc ttgaaagatt attgaaactg tcagaacagg cagagaagcg tttcgttccg
      421 ccctaccaca tcgcgctgat ttataacggt atggacgagc gcgacgaggc gtttgcgtgg
      481 ctggagcggg gagttgtaca gcgagacgcg aaaatgactt ttctaaaggt cgaaccgaaa
      541 tggaataatc ttcgctcaga cccacgcttc gccgaactca tgcgcaaagt cggactcccg
      601 cagtaacctt gaaaccgggc attaatctca cgccgccttc ctgcgtacct cttgcacacc
      661 tgaggctata aaatcccgtc gaaattgatt actcggcggt caagttgttg aatttttcct
      721 tgatgttttc cgctatccgc atcttgcgcc ggttggcgtc caccgcgacg tagacgactt
      781 cggcctccgt caccttgatg cgctcgctgg tgccgccgta gcgttcggct tcgacgacga
      841 cgcggatcgt gatcgaagtt gtgcccatgc gcacggtgct ggcgtagaag ctgacaaggt
      901 cgccgacgaa gacgggctgg tgaaagatga cctcgcgcat ggctaccgtg acgaaacgct
      961 cgatctgcgt gcggcggtgc gcttcgaccg cgcccgcgat gtctatgtag cttaaaatga
     1021 tgccgccgaa gatggtgccc tgcgggttgg tgtcgcgcgg caggagggtg agacggatgg
     1081 ccgggtcgct gtattccatg aatttttcgg gtgcggcgcg cgttgcccgc gtcgggcgag
     1141 acgaccgcca aagaccgctt cctgatcagg tttgtcagat ttgataaacg tgagagcatg
     1201 gacgcgcgtt cccacggctg tcaagccgaa ggcgaaaaag gagcgcgccg cgcagccaac
     1261 tgtgcccgcc ctgccctcat ctaaaatctt gccgcgccga agaatttttt tttaacgcct
     1321 cagtggattt tcgcacagtc cggtactgtg aagggcggcg cgacgctcgt atcgcaaaca
     1381 agatgcggcg agtataattg cccgcagcaa cgttaaattt agacttagtc ctccagaaaa
     1441 gattttatgc caaactcctt ctcgcgtttc tacgccgttc gcgcgcttct ggtcgcgatt
     1501 ttcgccgcgc tcgccttcgc ttcgaacgtc tccgcgcaac agcgcggcgg cggcacgcgc
     1561 ttcgacgtga ccaactatcg catcgaagcc gaactccgcc ccgccgagca cctcctgcgt
     1621 gccaacgccg acgtgacctt cacgccgctc gacacgacgc gctcggtcgt cttcgaactg
     1681 aacggcgcgc tcaaagtcga gagcatcgaa cgcaacggca agccgctcgc caatttcgtg
     1741 caagaccctg cgggactgga atccatcggg ccgaacgtgc gcgttgacct cggcgaagtc
     1801 gtcccggcga acacgcccgt cacgctgcgc tttcgctggt cgggcgcact cgtcacggcg
     1861 gaaggcggac ccctcgttac gcgtcgtctc gcctacgtcg gcaacgaagg ctcttacctg
     1921 atgtacgccg cgcgctggtt tccctttcac gactacgcgg ctgatcgcgc gacggcagac
     1981 atcaccgtta tcgtccccaa cggctttcag gttgcgggtt cgagcgacga gccgccgacg
     2041 caatcagcag gtgcggcggg caccacgcgt tatcgcttcg ttcaccgcca gcccgccctt
     2101 atcggcaaca tcgccgccgg gcgctacgta acgcgcacgc tccgcattgg cggctacgaa
     2161 ttgcagttct tcgtgcagcc ggggagcgaa gggcgcatcg agcgtttcgg cgaagtgttg
     2221 ggtcaggcgt tgcagtttta cacgaagcag tacggcgcgc ccgctttcgg cacgcgctac
     2281 accatcgcgc agattgacga cgacagcctt gaggcttacg ccgcgccggg gatgcagttc
     2341 ctgtcggcca gcttcttcga tccggcgcgc acgaccaaag aggaacgctt gcagcgcgag
     2401 gccgcctatc aatggtgggg ctttacggtc gccttgaaat ctttcgacga tgcgtggctc
     2461 tcgcaaggct tggccgagtg gagcgcgttc gccctgcgtg aatcgtcgct ctcgggcgcg
     2521 caactcgacg cctcgcagcg cgacatgcag gagcgcgcac tgatgttcga acagtcggcc
     2581 tcgatcctgc gcgcgccgtc cacgctcgac gatacctcgc cgcagtatca atccatcgtc
     2641 ttctacaaag gcgcgatggt cttccgcatg ctgcgcgaga caatcgggaa agagaagttt
     2701 gacgcgctgc tccgcacgct gttcgaacag tatcgcggca agagcgtctc catagacgat
     2761 ttcgaacgcc tcgcctcgca gcaggtgggc acgaacatgc gctacttctt cgcgcgctgg
     2821 gtggaaggca cgggcgtccc cgaattcacg gttgactacc agatcatacg cacgcgcacc
     2881 aacaagtttc gcgcacgcgg cacggtcaaa cagaacttcg agggcttgcg catgcccgtc
     2941 gaggtgatgc tgcgcgccga gggcgacaac cagaaccaga tcacgagtgt ccgcatcgag
     3001 gagaagagcg aggacttcga tttcgaatcg aacggcgcgc cgctcgaagt gatcgtcgat
     3061 ccgaacaaca agatcctgcg cctttcggaa gacctgcgcg tctcggtcgt cgcgcggcgc
     3121 ggcatcgaac tttatcgcga aggccagtat gccgaagcgc aacggcaact cgaagaggcc
     3181 ctcaagctcg acaagtcgaa ctcgtgggtc tactacaacc tcggcctgat ctatctcgaa
     3241 cagcgcaact acgatcccgc gctcgacaat tttcaggcgg ccttagacgg caacctcaag
     3301 ccttcctgga tcgaggtgtg ggcgcacatc aaacgcggca acgcctacga ctcgaaaggc
     3361 gaccgcgcac gcgccacgaa cgaatacaac aaggccgtcg aaaccggcaa caactacgac
     3421 aacgcgcaga aggtagccaa gcaatacctc gccacgcctt acgacccgcg cgccgcctcg
     3481 cagcaacaac aggcttcgag taattagttc aagacttcaa accttaaaaa ggccgctttc
     3541 agtttcaaca aactgaaagc ggccttttta gtctcctcgc gcacatccat ctcaaacaat
     3601 cggggaaggc gcaaggcagc ttagaaaaca acaactcgcc gggcgaggct tttctgttct
     3661 agccggcagt ccgcaatgga aaggtcaggc catcgctaca cgggcgggac ggcgacgatg
     3721 gcgatgcgtt gtttgtccgc cagccgcagc atctcttcgc ggtcgaacat gagcgtcttg
     3781 cccgccgtga tgcagaggca cgtcgcgccc gcttcgatca tgcgctcgat tgtgggcacg
     3841 ccgacgaccg gcacatcaaa gcgcatgtcc tgatccggct tggcgacttt gaccacggtg
     3901 agccgcccgc gcgcgaggcg tccggcgcgc tcgatacagg cgtccgtgcc ttccatcgct
     3961 tcgatggcga cgcaggcgcg cgcgcgcacg acgatggttt ggccgaggtc gaggcgtgcg
     4021 atctcgcgtg cgacttcgag gccgtattcg atgtcgccgc gttcgcgtgc gtcggcctcg
     4081 cgtttcgtta gtttacctgt ggcggggagg tgatcctgta ggaagtaggt ggagtctatg
     4141 agttcgatgc cttcgctgcg gagttcgtcg gcgatgccgc cgatgagcgc gtctgtgttg
     4201 cggcgcggga gccgcaggag cagcttgagc atccgcacgt ccggcagcgc gccgctgaaa
     4261 atctgcacgt gcttgacctg ccccgccatg atcgcgcgct ccacgccttc gcgtttgaag
     4321 aaacggatca tgcgcccgag ttgcccgatg cccacccact ccacgcgccc gcccgcgccc
     4381 gttgcggctt caatcgaagg atcagtctcc tcccgaatcg ccgccacaga caactctacg
     4441 cccgcgcgcc gcgccccctc gataacgagg aagggaaact tgccgttgcc tgcgatgagt
     4501 ccgtaacgca taagagaaca gtggcgagtg acgagtgaca agcgacgagc aagaaggaag
     4561 acaagtgaat cgtcaatcgt caatagacga aagcaggttc ttctctattt acgattgacg
     4621 attcacggct gtttaactcg tcacttgtca cctgtcactc gtcactgctt tatttgacga
     4681 ttccacgttt ggaggtttcg atgaagcgca agagttcttc cgtctcgaca gaatctttga
     4741 tctcggcgcg gatgcgcgcg agggcttgcg tggtgttcag gcgcgcgttg agcaggaggt
     4801 gaaaggcatg atggagcgcg gcgattgttt cgcgcgggta gttgcgccgc cgcatgccct
     4861 ctttgttcag gccgtagcac ttcgcatggt tgccgaccgt gagcgcgaaa gggagcgcgt
     4921 ctttgacgac gaccgaatag ccgccgacgt aagcctcgcg cccgatgcgg cagaactggt
     4981 gcacgccgga atacgcgccg atgtttgcgc cgtcggccac ggagacgtgc ccggcgagcg
     5041 tcgcggcgtt tgccatgatg acgcggttgc cgatgagaca gtcgtgcgca atgtgcgcct
     5101 gcgccataaa gagtccgtcg tcgccgattg ccgtgagcat cccgccgccc tccgtgccgc
     5161 gatggatggt gacgaactcg cgtatctggt tgcgcgcccc gatgcgcgtc tcggcaggct
     5221 cgcccctgta cttcaagtcc tgcggagcga ggccgatgga ggcgaagggg tagatatgcg
     5281 tctcgtctcc gatgtgcgtg cgcccttcaa tcaccgcgtg cgactcgacg cgcacgccct
     5341 tccctaaaac cacttcgtcg ccgatgacgc aatacggccc gataaagcag tccgcgctga
     5401 cctgcgctct gtcgctgacg atagccgtcg gatgaatcat aaataacgaa agaaggaaaa
     5461 aggggaaaga gtaaggggga aaggaaaaga cttcgcttcc cctctccctg cgacctttac
     5521 cccttcccca tttttactgg acgccgcttc gttcggccac gacgctcatg atctcggctt
     5581 cggccaccac gcgcccttcg accttcgctt cgcccctcat gcgttggacg cgcgagccga
     5641 tacgcaaagc cgtgacttcg agcacgagcg tgtcgccggg cacgacgggc tggcggaaac
     5701 gcgcccgctc gatgccggag aagaggacga gtttctcttc gcgccgctcg atctcgcgga
     5761 gcgcgagaat cgcccccacc tgcgccagag cctctacgat gagcacgccc ggcatcacgg
     5821 gtgcgccggg gaaatggcct tgaaagaatt gctcgccgat ggtcacttgc ttgataccga
     5881 cgatccgctc tttcgggacg agttcgatga tgcgatcaac gagcaggaaa ggataacgat
     5941 gcggcaggag ttcttgaatg gcgcgcgtgt cgtagacgat ttccatggga gtagtgagca
     6001 gcagcaagta gtaagcagta agcagcgagc agttgaaatt ttactgctca ctgcttactg
     6061 cttactgctc acctgtccgg ttacgggcga cccgcggggc gagccgctgc gccgcccgcc
     6121 ggagcggcgg aagcggtcgc gggcttcgcg cggttgtact cggcgatgaa ctctctggtg
     6181 atgtccaccg tgtcgttgaa gtagatgagc ggcacacggc tggcgtcgat gatgacggag
     6241 atgccgcgcg cttgggcgaa ggcttgcagc gtgttgtaga catcctcctg caacggcccc
     6301 aacacttcct gcatccgctt ctggatggag atctgcgcgt cctgcgtctt gcgctcgatg
     6361 tcgcgcttca tcgtgtcggc ctgatcaact ttggtggcga tggtcttcgg gtcggccacg
     6421 tctctggtct tgttgatgtc ggcgactaac gcttcgtagc gcgtctgcat gccttgcact
     6481 tcggtgcgca acggctgaaa ctgcgcgtcc acggtcttca tcgcgttgac gacgcgcccg
     6541 attccggcct tgtcgtcgct gaaggcagcg gagtcaataa cggcgatccg ccccgtgccg
     6601 gtggcagccg tgccgccgcc agcgggcgcg gcgggttgcg ggcgagcagg ggtctgtgcg
     6661 ctggcagaga gagagagaga ggccgcgatc acaaaggcgg cggcgataaa gcgaaatagt
     6721 ttcattgtct ttgttgtact gctccttaca gttgaaatgg gcggcgactt cttaggtgcg
     6781 cttggcttgc cattagttgc tgcgcctgaa cccgtagttt atcaagagac ggttaaaaag
     6841 tccgcccgat gctgaagcga tagccaatct tctcttcctg gaagaagagc ggaacctgtt
     6901 cgttgacgcc gcgccgtgcg ttcggattat agaagccgat cagcctgagc ggcacgttca
     6961 cgactggcaa ctgcacgcgc acttcgagtc cgacgctcga acgaaaatca ccgatcttat
     7021 cgaagaccgc ctgcgacagg cgcaccgccg tgttggtctg cgcgtcgccg cgcacgaaga
     7081 cgcgctggaa gccgaagggc agacccgtga tcggatcgag cgtacaggtg ttgcaagact
     7141 cctgcgcgaa ctcctcctgc gtcacgaggc gattgtcgcg cgcgagcagc ccgacatcga
     7201 acaggccgtt tcctctggac gtgatctgcg cggcgaggaa aggattcttt tccagcacca
     7261 gcgcgcctaa actaccgcga aaccctccga gctggctcgc gagaaagggg aaggccgagt
     7321 tgtcgttgtt gaaagagctg gcgaaaatct ggtctgcgcc ccgccgcagg ttgaaggccg
     7381 agccgatgtc tgcgaaaccg gcgatgctca cgggtccgaa gatgggcacg cgatattcga
     7441 agttgccgag cagttgcgtg tccgcgccga tgggctggaa gccgcgcgtc gggttggcga
     7501 tcacgtttcc gccctcagct ccggtgaagg tgccgagttg gcgcgcgagg ttttgcaggt
     7561 tcgcgctctc gacggggacg ggcgcgcccg ccgggttcga cgccagcacc acgttctgcg
     7621 aagtgacgaa ctgctcaatc ggcacgatgg ggctgatcga gcgcacgttg tagccgcgaa
     7681 tcgtgaattc gtcgccgagg aagaagcgtt cgtaaaccgg gacgccctga atgaacgaga
     7741 gcgaagtggt ctgcgcttcc ctgatcttcg cgctcgtggc gaaagccccg acgtggccga
     7801 ccagcaggcg gaaaccgaag acgtgcgggt tgttcgaacg cttgtttctg accggaatga
     7861 actgctggta agtaaggctc ggttgatagg tgcgcacgtc gccgccgagt cccgcgaagg
     7921 cgagttggac ggcgatctgc ttgccgccga tggcgtccac gccgcgcacg tcgcgcgagt
     7981 cgtagacgat gctcggcgtg aggcggctgg tcaggatgtt cggctgcctg tagataaccg
     8041 gaatggcgtt ctgcgggttg ctcggatcgg cgttgacttc cgggtccctg acgctcgttt
     8101 gcgagaggga gtaggacaag ccgatgcgcg tcgagagcgt aaagggacgc ttcttgtaaa
     8161 actcggagag cggcgcggtg gcgaagaaac tcgcgcccac cgtgcgctgc gtaaagagat
     8221 tttcgtcgcc cgtgttgagg aagtccaact gcgagccgga gagtccttgc agggcggcga
     8281 ggttctgcga caggaacgtg ccttcgccga agaacttgag cgactggacg aagaccgaga
     8341 agcccgcgga gatcggacga ttgcgaatct gcggctcggt gaacgagaag acgaacgact
     8401 gctggcgatt gcccgccgcg aagttgagcg acaagctctc gccgcgtccg agcaggttgt
     8461 tggtcgaata atcgaggccg aagaacgagc cgccgatgcc ggagaggccg ccgttgaaag
     8521 agatctgctg gcgtccgcgc tccgccacct tgacgttcac atcgacgagt ccttcttcct
     8581 cgttctggcg gaaatccacg tcggcatctt tatcaatcgg gtcgaagaag ccgagttggt
     8641 tgagacgcaa gaccgagtat tccacgtagg actggttata aatgtcgcct tcgttcagga
     8701 cgaactcgcg gcgcagaacg ttgtcgcgcg taaacgtgtt gcccgtgaat tccagacggc
     8761 gcagggtgaa ctgcttgccc tcgtcaatgg tgatcgtgta atcggcgatg ccttcttcgg
     8821 ggttcgcggg attgtccctg taggtcggct ccggctccgc cgtgtactgg atgaagccgt
     8881 tgttgccgta gagtttcttc aggtcttcga tcagaccctt gtagatgcgc tcgccgctcg
     8941 ccacgtcgcc ctgtttcaga ccgatgacgg cgcgaatcac cttctcgtcg aagatcgagt
     9001 taccttcaat cttgaggtcg ccgaggcgat agaccttgcc ttcgatgacg gggatcgtga
     9061 tgcgcagggc ttcgtctgtg gaagagagaa gcggcagcgg caggatcggg aaaccggtgc
     9121 ggcggcggcc taagccttcg atgcgcggct cgcccggtcg cccctgcaac tagcctttcg
     9181 aggccatgta gttgcgtatg aggcgcaggt caacatcgag tttttcgcgg tcgagaatgt
     9241 cctgcccttt gaaacgcgaa atcagccccg cctctttgac gtacttcatc gcgccgcgca
     9301 gttcgccctc tttaaacttc cggttgcctt caaattcgac ctcgacgacg cgcacgcggt
     9361 cgccttcgtc gatcttgaag gtgatcgaag tggaggtggc ggataccact tcctgctcaa
     9421 actcgacagt cgcgttcgga tgccctttgg cggcgagaag ttctttgatc agacgccgcg
     9481 cgttgttgag tttgacgggg tcgctgatgt tctctttctg aatgccgatg cgcttctcgc
     9541 ggaaggcttt gagcacgtcc gactcggtga tgctcgacag accgtcgaag accaactcgc
     9601 ggatgatcgg cagctcttcg acttcgaaga tcacttcgac gccgccgcgc ggccctgtga
     9661 gcgtcagcac gcgcgatttc gtcttatcga agaagttgag atcgttcagg gctttcaggt
     9721 cgcgcgccac ctgagcctcg ttgaaggggt cgcccgcgcg ggtcgtcacg taatagagca
     9781 aatcctcgtc gcggttgcgg cgattgccct ggatgtccac actttcgacc agtttttgcg
     9841 tctgcgcggc ggcaggggcg tccgtcggct cctgcgcgcg cacgtccgcc gggaaggcgg
     9901 tgagtaacaa cgccgtttgg ccgatgatca aagcggccag aagaaagcgc cgcaggggcg
     9961 cattaattcg cataattgct ttttgcctca aagcagaaaa agactttggg aagtaggaaa
    10021 acgaatcctg ttctgtgtac ccgccccacg gtcgcggcat gtagcgtaaa gacacgaaac
    10081 ggggtacaaa tcgagattga tttcggagtg ttggattcaa ctccgccgcg cctagttgcc
    10141 agccgtcggg tcgagcaaag gcgcgcatta tacaaggggg aaaggagaaa gggcaaaggg
    10201 cagggggcaa agtacccgcc gcgcggcact tgccttaccc tctaaccttt cccctctccc
    10261 ccttttccgg tcttttcagt gaattaaaag ttcgtcgccg agttcttcga cgcccgccgc
    10321 cggacgatgg ccgagcacgc cgccgtcctc ataaacctcg atcagcgtcg cgctgccgag
    10381 ttcgccttga atgagggctt cggagagcgg gtcttccacg tatttttgga gcgcgcggcg
    10441 caaggggcgc gcgccgtaag tgcggtcggc gcaggttttc ttgacgatcc actgcttggc
    10501 ctcttcggtc aggcgcactt ccagaccgcg acgacgcagc atgcggttca tcaattccac
    10561 ttgcagcccg acgatctcca gcaactcttc gtcctgcaac tcgtcgaaaa tgatgatctc
    10621 gtcgagacga ttgataaact ccgggttgaa ggtctgcttg acggaagaca tcaccatctc
    10681 ttcgagtttc tcgcgcgaat cgatggcgtg cgcctgaaaa ccgagcgcgc cgcgtttctg
    10741 tatgaagcgc gcgccgatgt tcgaggtcat gatgatgatc gcgtttttga aatccacggt
    10801 gttgccgagc gcgtcggtca ggatgccgtc ttcaaaaact tgcaggagca cgttgaagat
    10861 gtccgggtgc gccttctcga tctcgtcgaa cagaagcacg ctatagggcg aacgccggat
    10921 gcgctcggtc aattgaccgc cctcttcgtg gccgacgtag cccggcggcg agccgatcag
    10981 cttcgacacc gaatgcttct ccatgaactc tgacatgtcg aagcgaatca tcgagcgttc
    11041 cgatccgaac agaaactccg cgagcgaacg cgccacttcc gtcttcccca cgcccgtcgg
    11101 gccgaggaag aggaacgacc cgaccgggcg ttgcggattc ttcaagcccg cgcgcgaacg
    11161 gcgtatggaa cgcgcgagcg cggagatggc cggacgctgc gccacgacgc gcccgtgcaa
    11221 ctcgatctcg atgcgcagga gcttctgctg ctcctcttct ttcagagagt tgacgggaat
    11281 gcccgtccag cgcgcgatga catcctccac gtcgtctttc gtgacggaga cggagacgag
    11341 gatgtcgtcc tcttccgtga tgcgcgcgtc ggtcaaatta tccacgatgt aggaggcgcg
    11401 cagacgctcc tcgctgagag gggcgcgcgg gttgtcgcct aagccgggtt cgcggcgcac
    11461 gcgcaatttc acgcgcgctc cggcttcgtc aatcacgtca atcgccttgt cgggcaggaa
    11521 acggtctgga atgtagcggc tcgattgata gacggcggct tcgatggcct cttccgtgta
    11581 gcgcacctga tggaaagcct cgtaacgctc gcgcacgccg tcgaggatgc gcacggcctc
    11641 ctcttcggag ggcggcggaa ctttcaccgc ctgaaagcga cgctcaagcg agcgatcttt
    11701 ttcgatggac ttgcgaaact ctgcgggcgt cgtcgcgccg atgcactgaa tctcgccgcg
    11761 cgacagagcg ggcttcaaga tgttcgccgc gtcgagcgag ccttccgcgc tccccgcgcc
    11821 gacgagcgtg tgcagttcgt ctatgaagac gatgtactgc gggttctcga tgagttcccg
    11881 catgatctgt ttcagacgtt cctcgaactg cccgcgatac ttcgtgcccg cgacgatgag
    11941 cgacaggtcg agcgagagga tgcgcttgtt ctctaaaaac gaaggcacgt cgccgcgcat
    12001 gatccgctgc gcgagtcctt cgacgatggc cgtcttgccg acgcccggtt cgccgatgag
    12061 cacggggtta tttttcgtgc ggcggcagag aatctggatc acgcggtcga tctcgttgtc
    12121 gcgcccgacg agcgggtcga gtttgtcgtt cgcggcgtct tccgtcaggt cgcgggaaaa
    12181 ctctgcgagg tgcggcgtgt ccttcttctg cgaggcgcgc gggtctgcgc ccgtcgcacg
    12241 cgccacttct tcgcgcacgg cggcgaggcg caggccgcgc tcgtagagaa tctccgcggc
    12301 catcgaacgc tcttcgcgca ggaggccgag caggaggtgc tccgtgccga tgtggcgatg
    12361 ctgcaagcgg tcggactcct cgtgggcgta ggccagcacg cgcttggttt cgggcgcgag
    12421 cgggagttcg acgctggtgg aaatcttttc gcgcagaggg gcgcgccctt cgatctcttt
    12481 gcggatcgct tcgagcgagg cttgcgcacg ggcgaggaaa cgcgccgtga gcgttttgtc
    12541 ttcgcgcatc agaccgagca ggaggtgttc cggctcgatg gcgggcgcgc cgaattgcga
    12601 ggcttcgtag cgggcaaaga agatcactcg ccgcgctttc tccgtgtagc gttcaaacat
    12661 aaaaacagtt tggcggaagt cgcgttatga aaactttttg gtgtggcgcg tgatgaagta
    12721 aaaaaccatc ccgtatgccc gcgcgcatta taacctaatg cagcgaaagg cagaagagta
    12781 agaaaattta agcaggggaa aggagcgggg gaaaaggggc aaggggaagg ggtaaagggg
    12841 aaaggtaaag gcaaaacatt caccggcaca gacctgcttt cgccttccct tttcccttta
    12901 ccccttgccc ttccctcttg cctttactgc gtgagcgcga gcgagtcgag aaagcggctg
    12961 actttgtcgg gcgtcatctc gctttttgcg ccgagcacgc cgagggcgta gacggagcct
    13021 tcgacgacga agacgcgcgc gacggcgcgg tggccttcgg cggcaaactc caactcgcgc
    13081 ccttcgtagc cgtcgagcga gatggcctgt tccgagacga gccggaattt gatgttcggg
    13141 ttctcggcca gcagcgagcg ggtcatgtta ctcgccgcgc tcgcgaaaaa cttgtccatg
    13201 aaaccgggct gcgagacgag ttgtgcgggg agcggcggcg tgcgcgccac ttcatacttc
    13261 aagccaccgg cggcgagccg gtagctgcga aactgcgcgg ccagttgccc gctctcgcgc
    13321 ccgctctgcg tcggctcttc cggctgcccc ggcagggaga cggagaagcc cgcaccttcc
    13381 ggcgcaaacg tctgccacgc cgcgcccgcc atcgtcgccg ccgacacgcg cgccgaagtc
    13441 gaaggcagcg acaaattatc aagaccgccg cgccgcgtcc gcgctggctg ctgcgccgac
    13501 gccgcaagcg tgagcgcaca cagcgacacc gagagcgtca ggccgcgaaa tattttctgg
    13561 ttcacgagtt gtaactccta aaacttacgg gtggaaaaat ctatgaggtg agagcagggc
    13621 gggcgagcag ttcaaccgaa actgcccttc aatttttata gcatgcgcgg agaagaaaaa
    13681 gatagacgaa atttcccctg cgtgcaggtt agaccggacg ggcaaaatcg gcgtttaaaa
    13741 tttgcgcagg tagagagtcc acgcttttta cgtttgtgct ttgagcaggc cgtcttcgag
    13801 gaggagcgtg cggtcgcagg tgcgcgcgag ttgttcgttg tgcgtggcga cgatgacgcc
    13861 tgcgccgcgc gtgcgtgcga gcgtggagag cagtgcggcg acgctctcgc ccgtgcgcgc
    13921 atcgagattt ccggtcggct cgtcgcagag caggaggcgg ggcgcatgga caagggcgcg
    13981 tgcgattgcc gtgcgttgct gttcgccgcc tgaaagttcg ccgggacggt gcgccgcgcg
    14041 ttcgctcaaa ccgaccgccg cgagcagcac gcttgccgcc tcgcgcgcct cacgcggttt
    14101 gcgccgcgcg acgaggagcg gcagcgcgac gttctccagc gcactcaggc cgggcagcag
    14161 gtggtgaaat tgaaagacga agccgacctg ttcgccgcgc acgcgcgcca gttccgcccc
    14221 tacggcgcgc gtcagttcga tctcgccgag ccggacgctc ccgccgtccg ccgcgtccaa
    14281 cccgccgagc aggtgcagca gcgttgactt gcccgcgccc gacgcgcccg tcaccgccag
    14341 catctctccc gccgggagcg taaaggagac gccccgcaac acctcgaccg ccccgcccga
    14401 aggcgacgcg taagccttgc gcagttcgcg cacttccagg caagtctcaa gtctcaaatt
    14461 tgaagtccgt agtctcaagt ctcaagttcg cagtctcaag tctcaagccg ggcgggcatc
    14521 gagactacgg acaacggact ctggactttc attcgtaacg caaagcctcc gccggacgga
    14581 ggcgagccgc cgcgcgtgcg gggtagatgg ttgcgagcaa gctgatgatg aaggcgaaga
    14641 gcgcggccag aaccacgtcg cccgcgtgcg ggtgaaaggg gatcgcgctg agcgaataga
    14701 catctgcggg cagggagacg agtttgaagc gttcgccgat gaagcaggcg gcgaggccga
    14761 gcgcgacgcc tgcacacgcg cccaccgcgc cgatgatcgc gccctcgcag atgaagatga
    14821 gcatgatgct gtgcgggcgt gcgcccatcg cgccgaggat ggcgatgtcc gggcggcgtt
    14881 cgatgacgac gagcacgagc gtcgtcgtga tgttgagcgc ggcgacaaac atgatgagcg
    14941 cgatgatcag cgcgacggtg cggcgttcga gttggagcgc ggcgaagagc ggggcgttca
    15001 cctcctgcca gtcaaccgtc ttgaagtttg cgccgagcgc atcgcgcacg cgcgccgccg
    15061 tctcgcgcgt acggtagatg tccgccgtct cgatgctgat gacgggcgcg cccggctgcg
    15121 cgcctttctc tcccttggtg tgtgggagcg ggacataaac ccacgtcgca tcgtattcgt
    15181 agaggccgga gcgaaagagg ccgacgacgc gcacgcggct cgtctgcgtc acaaaccagg
    15241 gcggctcgga gaacctctgg ccggtgatga gccagccctc gtcgcctatg cgtcgcaagc
    15301 ccgtgcgtgc ggccagttcg gcgccgatga cgatgggcgc gggcgacgca tcctgctcgt
    15361 ccggcaacgt gctgaacaaa gttcccgtgt ccggctccgt ttcgcccggc accggcgtta
    15421 cgggataacg tctcaactga gggtcggggc gttcagcgtc ttgcgtctcc tcgaacagag
    15481 gctcaatcgc gccttcgacg agcgtgcggc gaatttccgc gaccgcgcgc gcggacgttt
    15541 tttccacccc gcgcaggatc gtgtacgagt cgccgcccgc gccgctcagg agcgcgcccg
    15601 tgtaactcgt cggcgcggca tccaccacgc cctcgatggt gcgtagccgc gcgatcacgg
    15661 cgggcgtgtc ttcgatgagt ttgccgccat cggcgcgcgt gagcgtgatg tgcgccgtgc
    15721 cgccgaggat tttggcgcgc atctcgtctt gaaagccgtt ggagagcgcg agcgcgaaga
    15781 tgagcgcgcc gacgccgcag gcgatccccg ccacgcccgc cagcgcagtc acgcgcgcgc
    15841 ttcgctgccg cccgcgccgc ttcgagcgtt gcagataacg cagggcgaga aaaagttcga
    15901 aaggcataaa gacgaaggag tcaggggaca gaattcagaa tacagaactc agaactcccg
    15961 gcagtcagat attcagtaga gaagttcacg ggcgtctgac ttctgaattc tgagttctga
    16021 gttctgagtt ctgtactcgc ttccgcgttt agcgaaagag cgacttcgga ttgccgagca
    16081 ggcggcgcag gttcggttcg tcgcgcgccg tctggtcgcc gggcggacga atctcgtaga
    16141 gcgtctcgga gaagcgacgg aagcgaaagc gcgtctccag attgacgagg gcgcgcgtct
    16201 cctgctcgtg ccaactgtcc atcgaccaga gacgcggatc ggtgtctaca aggacgcgat
    16261 ggccttgtgt caggtgccat tcgatctgct ccgggagtcg ctcggctccc ggccagccgc
    16321 cgcccgtgcc gataacgtcc cactggccta gaccgaggcc gcgataataa ttgaccgcca
    16381 ccgtctgccc gcccgccatc acgaccgcat ccttcggcag agagatcagc cgcgcccggt
    16441 actctttcgt caacgcgtgg ctggctagcg tccgcgccgc gaagatgtag acgagcgttc
    16501 cggtc
//
LOCUS       DASWNS010000579         8398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_126043, whole genome shotgun sequence.
ACCESSION   DASWNS010000579 DASWNS010000000
VERSION     DASWNS010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126043"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1599
                     /locus_tag="VGB76_20010"
     CDS             160..1599
                     /locus_tag="VGB76_20010"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0101220.1"
                     /translation="MKIVWRAASGAMCLFALLILGGCQAAPVSNVNTATNATPAPGAT
                     AATPANANAGANNVPLTLPLVDAMFADENFAKDLKSELGLTDEQIERLRKVAREETGS
                     LRETETGEHGGTTTAATTRAAEQIRSVLGEEKAARFSEFARARWAGGESGTDAAGGAT
                     GGGKPNAVPSDTRIVVNAPAYRMDVFENGQLVKSYRIGIGYPEFPLPTGLRRANTIIF
                     NPTWTPPDEPWVESPSSKVKVGEKVDAGSPLNPLGLIKIPIGSPSLIHGGKAAAKLGT
                     FASHGCVGLTNAQVQDVARVMAKIGGAQLTDEDVATYAKNRKETKEVKLERPVPVELR
                     YETIVVEDGKLYIYRDVYDRDTNTEENLRAVLAANGVSFESLSEQERAQVLDALRQMA
                     RDATGKPADGQPDASPGASPEAANKNASRNGNKNANAESGKVTRTIKGQKEIVVELAA
                     LRGKGYPAPVALDTGGASKETGGKKAKRG"
     gene            complement(1731..2639)
                     /locus_tag="VGB76_20015"
     CDS             complement(1731..2639)
                     /locus_tag="VGB76_20015"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0101221.1"
                     /translation="MWKKFIFNLWANKSFGWRMARIVLLLCAGLAGYLMLFEDSFIYF
                     PAKYPAGAWERAELRAREGEIVARVEDVQLVAADGVRLHGWYGTPQVGRGGQLEPVRA
                     DSTLLFLHGNAGNISHRYEIVRDFVKLPVNVLIIDYRGYGKSEGRPSEQGLYTDARAA
                     WDYLTTTRAVPASRIVIFGESLGGAVAIDLASKTNACGLVVQSSFTSIGDMAAELMPF
                     IPRFMLRTKMDSLSKITSVSCPKLFIHSQADEIVPYKLGRRLFDAAPAPKQFYEVKGA
                     THNLMFELGGAAYYEALRGFINSSCK"
     gene            complement(2788..4287)
                     /gene="hutH"
                     /locus_tag="VGB76_20020"
     CDS             complement(2788..4287)
                     /gene="hutH"
                     /locus_tag="VGB76_20020"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY0101222.1"
                     /translation="MIELNGQPLSLEQIASVARGAERVSLAAEARVRCAAARALVERI
                     VEEERVVYGVNTGFGKLSDYSIPRAELRELQLNLVRSHSCGVGSPLSAEETRAMILLR
                     ANVLALGYSGARTVVCETLIEMLERGVVPIVPEKGSVGASGDLAPLAHLALACIGEGE
                     SVYEGTRMTSAEALSRTGIQPLTLEAKEGLALLNGTQALTAVGALALRRAEQLAHAAD
                     IAGAMTLEALRGTPAAFDPRLHSARPHPGQVAAATHLRELLRDSEIRESHRENDPRVQ
                     DAYSLRCMPQVHGAARDCFAHARATVEIETGSATDNPLVFADTDEIISGGNFHGAPLA
                     LAFDYCGIALTHLLNISERRIERLINPDMNEGLPAFLTAHPGTSSGFMIAHVTAVALL
                     NEMKVLAHPATTDNLPTSAGKEDHVSMGMTAATKFRSMVYNAEHVLAVELLAAAEGLE
                     YRAPLKPGLGVSRAYELVRAHAPRLTRDRPLSSDIERLAAVIRRGEFNF"
     gene            complement(4448..5452)
                     /locus_tag="VGB76_20025"
     CDS             complement(4448..5452)
                     /locus_tag="VGB76_20025"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HEY0101223.1"
                     /translation="MRPRPPTGPLPGGERQNTGSFRSPVTPTKETAAPNSPDSFTLLG
                     RKISPAEFNTAMVHFYRGEVQRSNTWRNRLDTTTNWAVLTAGATLSFAFSSAENPHFV
                     IPINSVLVAFFLFMEARRYRYYEIWASRVRLLETGYFAQMLAPTAVQDQEWAEHLAAD
                     MRAPHFTISEWEAVGRRLRRNYLWIFILLAVSWNLKVYLHPFPARGLEMFIKRAAIGS
                     LSGEFMVLFGIIFNTVVLVFAFATVRLKQATGEVLPQHEFPFHPIRSVTSTITRAAVQ
                     RRVTERRTRKARQRTRTTRPGSGMTGSLTGSTTGEWKRPDTGDLKRTDREPVTEETLT
                     "
     gene            complement(5454..6539)
                     /locus_tag="VGB76_20030"
     CDS             complement(5454..6539)
                     /locus_tag="VGB76_20030"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEY0101224.1"
                     /translation="MPKLILHARAPFDFAATARFLRFTDAEVVDTFTSARYRRAIHFG
                     ERLFLLTVASPEDASEPLVDASESPVDANTRRSREEAGGRPAVEITLAPEPSAPTEVF
                     DEAARTVRGMFSLEHDLAAWRTRLARDPLMRRLEADHRGLHLPRWPTLFEALLTSILL
                     QQIATGVAITFRRRVVERFGAQLEVEGQRYFAFPRAERLARASVEELRALGLSNAKAT
                     CIVEVARACAAGELSSASLAGEDNETIIARLSSLRGIGRWTAEWVLMLHFGRADVFPA
                     ADLFLRGVVVKYYNKGATLDEREIRTFAEKRWGAYQSYSALYFLAGMRAGTITLKPER
                     VLSSRQRAGTPAIERAARKTTNRQDET"
     gene            6658..7149
                     /gene="tadA"
                     /locus_tag="VGB76_20035"
     CDS             6658..7149
                     /gene="tadA"
                     /locus_tag="VGB76_20035"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY0101225.1"
                     /translation="MFETEAEEEADSRWMRVALEAARDAGTERDEVPIGACVVSAQGT
                     LLAVAGNRTRTDCDPTAHAEIIALRAAAREIENYRLTGAIVYSTIEPCAMCAGALVQA
                     RVRRLVYGARDERAGAVASKFRICDDSSLNHRMELKAGVLEAECRELMQAFFRARRRR
                     NDE"
     gene            7547..7939
                     /locus_tag="VGB76_20040"
     CDS             7547..7939
                     /locus_tag="VGB76_20040"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0101226.1"
                     /translation="MPVQRITAGARFTIYAWGDKTSCEVLEFLEGLAANGDSDAERIL
                     NLIKRTADHGAPRNEQHCRLLEDSIYELKAPNTARVLWFYDAGSIIICTHGFSGKRGR
                     GKTSKSEIERAKKIRELYLKEKDHASRN"
     gene            7923..8378
                     /locus_tag="VGB76_20045"
     CDS             7923..8378
                     /locus_tag="VGB76_20045"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0101227.1"
                     /translation="MLHATKQETVAERFTNLFERFEQSETYHIDAAKLEISEQIYLTM
                     EQQGVSKAELARRLGKSRAYITKVLQGSTNFTLESLIRVARALDCQLDLQFSPRHKAS
                     ELDELWEAGRRSASHSKMPFRPSSEYIKLIETPLRVTTEIKDAPIAVAA"
BASE COUNT         1690 a         2450 c         2674 g         1584 t
ORIGIN      
        1 cgtccgttaa tcgtgcgagc gtgggcgggc gtgaatcgtg aaaagacgtg aaatcgcgtt
       61 gattacaggc ggcgcgccgg tgggaacgag tgttgctatc agatgtgggg atgcgccggt
      121 gtgggcgcga aagtcttttc cggaggagct aaagccagca tgaaaatagt gtggcgcgcg
      181 gcgagcgggg cgatgtgcct gttcgcccta ctcattctcg gcggttgtca ggccgccccg
      241 gtttcaaacg tcaacacggc aacgaacgcg acgcctgcgc cgggcgcgac cgccgccacg
      301 cctgcaaacg ccaatgcggg cgcaaacaat gtcccgctca cgctgccgct cgtggacgcc
      361 atgtttgcgg acgagaactt tgcgaaggat ttgaagtcgg agttggggtt gacggacgag
      421 cagatcgagc gtctgcgtaa agtcgcgcgg gaagagacgg gcagtttgcg cgagacggag
      481 acgggcgagc acggcggcac gacgaccgcc gccaccacgc gcgccgccga acagatcagg
      541 agcgtgctcg gcgaggagaa agccgcgcgc ttctccgagt ttgcgcgcgc gcgctgggca
      601 ggcggcgaga gcggaacgga cgcggcgggc ggcgcgacgg gcggcggcaa gccgaacgcc
      661 gtgccttcgg acacgcgcat cgtcgtcaac gcgcccgcgt atcgcatgga cgtgtttgaa
      721 aacggtcaac tcgtgaagag ttatcgcatc ggcatcggtt acccggaatt cccgctcccg
      781 acgggcttgc gacgcgcgaa cacgatcatc ttcaacccga cgtggacgcc gcccgatgag
      841 ccttgggtcg agtcgccttc gagcaaggtc aaagtgggag agaaggttga cgcgggcagt
      901 cctctgaatc cgctcggcct gatcaaaatt cccatcggct cgccctcgct catacacggc
      961 ggcaaggccg cggccaaact cggcacgttc gcctcgcacg gctgcgtcgg gttgaccaac
     1021 gcgcaggtgc aggacgtggc gcgcgtgatg gcgaagatcg gcggcgcgca gttgaccgat
     1081 gaagacgtgg cgacctacgc gaagaatcgc aaggagacga aagaggtcaa actcgaacga
     1141 cccgtgcccg tcgaactgcg ctacgaaacc atcgtcgtcg aagacggaaa actctacatc
     1201 taccgcgacg tgtacgaccg ggacacgaac acggaagaga acctgcgcgc cgtgctcgcg
     1261 gccaacggcg tctcgttcga gagcctgagc gaacaggagc gcgcacaggt tttggacgcg
     1321 ctccggcaga tggcgcgcga cgcgaccggc aaacccgcag acggccaacc cgacgcttcg
     1381 ccgggcgcgt cgcctgaagc cgccaacaag aatgcgagca ggaatggaaa caaaaatgcg
     1441 aacgccgaaa gcggcaaagt aacgcgcacg atcaagggac agaaagagat cgtcgtcgaa
     1501 ctcgccgcgc tcagaggcaa aggctatccc gcgcccgtcg cattagacac gggcggcgcg
     1561 tcgaaagaga cgggcgggaa aaaagcgaag cgcgggtaag acagttagcg tgttgcgtcc
     1621 tgtagggaca tttgaaaata gccctgcgat tcgtcgtcgg gctattttca aacgcgcttt
     1681 acgggaactg aaatcggttg tgagttattc gcgtcggact ttcctgaatc ttatttgcag
     1741 gatgaattga taaagccgcg caaggcttcg taataggctg cgccgccgag ttcgaacatg
     1801 agattgtgcg tcgcgccttt gacttcgtag aactgtttcg gcgcgggcgc ggcgtcgaac
     1861 aggcggcgtc cgagtttata agggacgatc tcgtcggcct gactgtggat gaaaagtttc
     1921 gggcaggaga cagacgtgat cttcgagagc gagtccatct tcgtgcgcag catgaagcgc
     1981 gggatgaacg gcatcaactc cgccgccatg tctccaatcg aagtgaagct cgactgcacg
     2041 acgaggccgc acgcgtttgt cttgctcgcc agatcaatcg ccaccgcgcc gccgagcgat
     2101 tcgccgaaga tgacgatgcg cgaagcggga acggctcgcg tcgtcgtcag gtaatcccag
     2161 gccgcgcgtg cgtctgtgta taagccctgc tcggacgggc ggccttcgct tttgccgtag
     2221 ccgcgatagt cgatgatcag gacgttgacg ggcagtttga cgaagtctct cacgatctcg
     2281 tagcgatgcg agatgttgcc cgcgttgccg tgcaggaaaa ggagcgtcga gtctgccctg
     2341 acgggttcga gttgtccgcc gcgcccgacc tgcggcgtgc cataccagcc gtgaaggcgc
     2401 acgccgtccg ccgccacaag ttgcacgtct tcgacgcgag cgacgatctc tccctcgcgc
     2461 gcacgcaatt ctgcacgctc ccacgcgccc gccggatact tcgccggaaa gtagatgaaa
     2521 ctgtcctcga acaacatcag ataccccgcc aaacccgcgc acagcagcaa cacgatgcgc
     2581 gccatgcgcc acccgaaact cttattcgcc cagagattaa agataaactt tttccacatg
     2641 cgtttccatc ccgcgccccc gcgagaagat taaacgccag ccgcgctgaa agtggaaagt
     2701 ttttctttgc gcccccggcg tcagaccgaa ctgcacgcgg gaggcgcaaa gacacaagcg
     2761 ggaagcatga agtttgattg aactgtttta gaaattgaat tcgccgcgac ggatgacggc
     2821 ggcgaggcgt tcgatgtcgg aggagagcgg gcggtcgcgc gtgaggcgcg gggcgtgtgc
     2881 gcgtacgagt tcgtaggcgc gcgagacgcc gaggccgggc ttgagcgggg cgcgatattc
     2941 gagtccttcg gcggcggcca gcagttcgac ggcgaggacg tgttcggcgt tatagaccat
     3001 cgagcgaaat ttcgtcgcgg cggtcatgcc catcgagacg tggtcttctt tgcctgcgga
     3061 ggttggcagg ttgtccgtcg tcgccgggtg cgcgagcact ttcatttcgt tcaggagcgc
     3121 aaccgccgta acgtgcgcga tcataaaccc ggaggacgtg ccgggatgcg cggtgagaaa
     3181 ggcggggagt ccctcgttca tgtccgggtt gatgagacgt tcgatgcggc gttcgctgat
     3241 gttcaggaga tgcgtgagcg cgatgccgca atagtcgaac gcgagcgcga gcggcgcgcc
     3301 gtgaaagttt ccgccggaga tgatctcgtc cgtgtctgcg aagacgagcg ggttgtcggt
     3361 cgcactgcca gtctcgatct cgaccgtggc gcgtgcgtgc gcgaaacagt cgcgtgccgc
     3421 gccgtgcacc tgcggcatgc agcgcagcga ataagcgtcc tgcacgcgtg ggtcgttctc
     3481 cctgtgcgac tctctgatct cgctgtcgcg cagcagttcg cgcaggtgtg tggcggcggc
     3541 cacctgaccg ggatgcgggc gcgcgctgtg caggcgcggg tcgaaggctg cgggcgtgcc
     3601 gcgcagggct tcgagcgtca tcgcgcctgc gatgtcggca gcgtgcgcga gttgttcggc
     3661 gcggcgcagg gcgagcgcgc cgacggcggt gagagcctgc gtgccgttca agagcgcgag
     3721 tccttctttg gcttcgagtg tgagcggttg gatgccagtg cggctgagag cctccgcgct
     3781 cgtcatgcgc gtgccttcgt acacggactc gccttcgccg atgcacgcga gcgcgagatg
     3841 cgcgagcggc gcgagatcgc cgcttgcgcc gaccgaaccc ttctccggca cgattggcac
     3901 cacgccgcgc tccaacatct cgatgagcgt ctcgcagacg accgtgcgcg cgccgctgta
     3961 gccgagcgcg aggacgtttg cgcgcaacaa gatcatcgcg cgcgtctcct ctgcggagag
     4021 cggcgagccg acgccgcacg aatggctgcg cacgaggttc aactgcaact cccgcaactc
     4081 ggcgcgcggg atcgagtagt ccgacaactt cccgaagccc gtgttgacgc cgtacaccac
     4141 gcgctcctct tccacgatgc gttcgaccag cgcacgcgcc gccgcgcacc tcacgcgcgc
     4201 ctccgccgca agcgacacgc gctccgcccc gcgcgccacg ctcgcgatct gttcaagcga
     4261 caacggctgt ccgttcaatt caatcataag aagacagtga cgagtgacga gtgacgagat
     4321 taaaagagta aatcgagatt aaaacagtga atcgtgaagc gtagatcgtg agttgagaaa
     4381 acctgtcttc ttctattcac gattgacgct tcacgattca cgtcttttta actcgtcact
     4441 cgtcacttca tgtaagcgtt tcttctgtga ccggctcgcg gtctgtccgt ttgagatcgc
     4501 ccgtgtcggg gcgtttccat tcgccggtcg tgctgcctgt gaggctgccg gtcatgccgc
     4561 tgccgggacg cgtggtgcgc gtgcgctggc gcgcttttct ggtacggcgt tcggtgacgc
     4621 ggcgttgcac ggcggcgcgc gtgatggtgc tcgtcacgct gcgaatcgga tggaagggaa
     4681 actcgtgttg cggcagcacc tcgcccgtcg cctgtttcag gcgcacggtg gcgaaggcga
     4741 agacgaggac gaccgtgttg aagatgatgc cgaacagcac catgaactcg ccggagagcg
     4801 aaccgatggc cgcgcgcttg atgaacatct ccagaccgcg cgcgggaaac gggtgcaagt
     4861 agactttcaa attccaactc accgcgagca ggataaaaat ccagagatag ttgcggcgca
     4921 agcgacgccc gacggcctcc cattcgctga tcgtgaagtg gggcgcgcgc atgtccgccg
     4981 cgagatgctc cgcccattcc tgatcctgca cggctgtcgg cgcgagcatc tgcgcgaagt
     5041 agcccgtctc cagcaggcgc acgcggctcg cccagatttc gtaatagcga taacggcgcg
     5101 cttccatgaa gagaaagaag gcgacgagca cagagttgat tggaatgacg aagtgcggat
     5161 tctcagccga actgaaggcg aaggagagcg tcgcgcctgc ggtgagcaca gcccagttgg
     5221 tcgtcgtgtc gagacgattg cgccaggtgt tcgaacgctg cacctcgccg cgatagaaat
     5281 gaaccatcgc cgtgttgaat tcggctggcg aaattttgcg accgagcagt gtgaaagagt
     5341 ctggggagtt gggagcggct gtctctttcg tgggcgtgac gggagagcga aaactgcccg
     5401 tgttctgtcg ttcgccgccg ggtagtggtc cggtcggtgg tctagggcgc atctcaggtt
     5461 tcatcttgtc ggttggtcgt cttcctcgca gcgcgttcaa tcgccggagt gcccgcgcgc
     5521 tgacgggatg ataacacgcg ctccggtttg agtgtgatag ttccggcgcg catccccgcc
     5581 agaaagtaga gcgcgctgta agattgatac gcgccccaac gcttctccgc aaacgtgcga
     5641 atctcccgct cgtcgagcgt cgcgcctttg ttgtaatact tgacgacgac cccccgcagg
     5701 aagaggtctg cggcgggaaa aacgtctgcc cgcccgaagt gcaacatcaa gacccactcc
     5761 gccgtccagc gaccgatgcc gcgcaaactt gagagccgcg cgatgatggt ttcgttatcc
     5821 tcgcctgcga gcgatgcgga actgagttcg cccgccgcgc acgcgcgcgc cacctcgacg
     5881 atgcacgtcg ctttcgcgtt cgacagcccc agcgcgcgca actcttcgac gctcgcacgc
     5941 gccagccgct ccgcgcgcgg gaaggcgaag tatctctgac cttcaacttc gagttgcgcg
     6001 ccgaaacgct cgacgacgcg acgcctgaac gtgatcgcca cgcctgtcgc aatctgctgc
     6061 aagaggatgg aggtcagcaa tgcttcgaag agcgtcggcc agcggggcag gtgcagtcct
     6121 cgatggtcgg cttcgagccg acgcatcaga gggtcgcgcg cgagccgcgt gcgccacgct
     6181 gcgaggtcat gttcgaggct gaacatccca cgcaccgtcc gcgccgcttc gtcaaaaact
     6241 tctgtcggcg cggacggctc cggcgcaagc gtgatctcga cggcgggacg cccgcccgcc
     6301 tcttcccgcg accgtcgcgt gttggcgtct accggcgact cgcttgcgtc taccaacggc
     6361 tcgcttgcgt cttccggcga tgcaactgtg agcaggaaaa gtctctcccc gaagtgaatg
     6421 gcgcgccggt agcgtgcaga cgtgaaagtg tccaccacct ccgcgtcggt gaagcgcagg
     6481 aagcgcgccg tggccgcaaa atcgaagggc gcgcgggcgt gcagaatgag tttcggcatg
     6541 cctccgagtg tcgcagaacg gggcgcgaag cgtcaaagtc tatgcgcttg acagcgggcg
     6601 cgggcgtgcc tatagtcagg ttttgtctgc tgctgattga aagcgtctgt gcgtgatatg
     6661 tttgagacag aggcagagga agaggccgat tcacgctgga tgcgcgtggc gttggaggcg
     6721 gcgcgcgacg cgggcacgga gcgggacgag gtgcccatcg gcgcatgcgt cgtcagcgcg
     6781 caagggacgc tcttagccgt cgcgggaaac cgcacgcgca ccgactgcga cccgaccgcg
     6841 cacgcagaga tcatcgccct gcgcgcggcg gcaagagaga ttgaaaacta caggctcacg
     6901 ggcgcgatag tctattcgac cattgagccg tgtgcgatgt gcgcgggcgc gctcgtgcag
     6961 gcgcgcgtga ggcgtctggt ctacggggcg cgagacgaac gcgcgggcgc ggtcgcctca
     7021 aagtttcgca tctgcgacga cagttctttg aatcatcgaa tggaattaaa ggcgggcgtg
     7081 ctcgaagcgg aatgccgcga actgatgcaa gcattcttcc gtgcgcgacg ccggcgaaac
     7141 gatgaatgat gaatgaaaga caaaggcttt tacttcatcc ttcatctctc atccttcatc
     7201 gcttctcatg cggagaggtc gcatagtggc ttagttatga cgatctcgaa aagcggtggt
     7261 tacacggttc ggtgatctct ttctcacgct cttttcctca gctttctggc aataacggca
     7321 agtcatccgg cctgccgttt ttcttttgtg agcagcttat gatcatcttt cccgtcctgc
     7381 tctgtttttc agcgatagcg agggccttgc gctcgtcggt ggcgtacgca tgatgcctgc
     7441 gcatctccgt atttctgagg cggtttttaa cttgttattg caataattgg gctaaatccg
     7501 gcctgatttc cgtcgcttga cacttgtaaa cctataggtt aacatcatgc cggttcagcg
     7561 gatcacagcg ggagccagat ttacgatcta tgcgtggggc gataaaacgt cgtgcgaagt
     7621 gcttgaattt ttggaaggat tagctgcgaa tggtgattca gacgccgagc gcattcttaa
     7681 cctcatcaag cgtactgccg atcatggagc accacgcaac gaacagcatt gccgattgtt
     7741 ggaagatagt atttatgaat tgaaagctcc gaacacggcg cgcgtccttt ggttctatga
     7801 cgctggaagt atcattattt gcacacacgg tttcagtggc aaaagaggac gcggtaaaac
     7861 gtccaagagc gagatcgagc gcgccaagaa gattcgagag ttgtacctga aggagaaaga
     7921 ccatgcttca cgcaactaag caagaaacag ttgccgaacg ctttactaat ttgttcgagc
     7981 gctttgaaca atccgagact tatcacattg acgcggcaaa gctcgaaatc tcagaacaaa
     8041 tttatttgac aatggagcaa caaggcgtct ctaaggcgga gcttgcccgt cgcctgggca
     8101 agagccgcgc ctacatcacc aaggttcttc agggcagcac caactttacc ctagagtcgc
     8161 tgatcagagt tgcgcgtgcg ctggactgcc agcttgatct ccaattctcc cctcgccata
     8221 aagcctctga acttgacgaa ctgtgggaag caggccggcg gtctgcctcc cacagcaaaa
     8281 tgccattcag acctagtagt gaatacatta agctgatcga aacccctttg agagtgacta
     8341 cggagatcaa agatgcgccc atcgccgttg cagcttgagt cttactacgt cacagaac
//
LOCUS       DASWNS010000580         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_48034, whole genome shotgun sequence.
ACCESSION   DASWNS010000580 DASWNS010000000
VERSION     DASWNS010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48034"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGB76_20050"
     CDS             <1..463
                     /locus_tag="VGB76_20050"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY0101228.1"
                     /translation="VEFYNDSKATNVDAALKALEAFAEHTGQIVLILGGRGKNAPYAP
                     LAPLIERKARALVVLGEDAERIEHELKAHAHVSRVRDLGEAVRRAFAAAQPGDVVLLA
                     PACASWDMFESFEHRGRVFKEEVGKMMNSEHRIQKPEEMQVAASAILNSDS"
     gene            481..1683
                     /gene="ftsW"
                     /locus_tag="VGB76_20055"
     CDS             481..1683
                     /gene="ftsW"
                     /locus_tag="VGB76_20055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY0101229.1"
                     /translation="MARKLQADEWLFAATLGLALFGVVMVYSASADLAYVENGTQYHY
                     VKRQAMWTVAGLLAMMAAMHFDYGWLRDRRVVAGMLAGTVVLLIAVFAFPRINGAHRW
                     IRFGGFSLQPSELSKLALTIFLARFLERRAGEENALWKTFMPCVLMTGLLVMLVAAEP
                     DLGTAMMLGIICFTLLFAAGARLLYLGAIGAVGVVGIVFMIVLFPWRLGRVTAFLDPW
                     ADAQGTGFQVVQSLIAVGSGGTNGLGFAQGRQKMLFLPFAHSDFIFAVVGEELGLIGA
                     LTLVLVFGLFLWRGVRAARRAPDRFGMLLGLGIVIGIVSQALFNISVVLSLVPTKGIP
                     LPFISYGGSSMMLTLVGVGVLLNISQQGTAQGDAATRKEFAAESVPATPRRERAPVTQ
                     TWRPEGRS"
     gene            1659..2768
                     /gene="murG"
                     /locus_tag="VGB76_20060"
     CDS             1659..2768
                     /gene="murG"
                     /locus_tag="VGB76_20060"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY0101230.1"
                     /translation="MATGRPELSLKMRALFAAGGTGGHIYPALAVAKELVSRHEDSAV
                     RFVGTARGLETRLVPQAGFKLSLIESAGLTNMSLGARLRGLWLLPKSFAAARSLIREF
                     RPDVVVGAGGYVTGSVLLTAALMRIPTLVMESNAVPGFTNRRLARFVDAATVSFEATL
                     PYFRGKAIVTGNPVRREFFDIPAKMRDPSRFSLLLFGGSQGARAVSNAMVAALPHLAA
                     YRDVLHITHQTGRLDYEAVRAGYEQAGWTERAEIKEYIDDMVGEFARADLIVSRAGAT
                     TAAELMAAGKTAIMVPLPGQLEQRRNAEVLQDANAARMILQESLTGETLAKEIAELIN
                     DPGEITRREEASRKMARPNAAALTVDLIEKLVGRR"
     gene            2857..>3060
                     /locus_tag="VGB76_20065"
     CDS             2857..>3060
                     /locus_tag="VGB76_20065"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEY0101231.1"
                     /translation="MFNRIQHVHLVGIGGIGMSGIAEVLCNLGFQVSGSDIRRSSATE
                     RLEGLGVTVKEGHRAENVGDAQVV"
BASE COUNT          520 a          841 c         1067 g          632 t
ORIGIN      
        1 ggtcgagttt tataacgact cgaaggcgac caacgtggac gcggcgttga aagctctcga
       61 agccttcgcc gaacacacgg ggcagatcgt cctgatactc ggcgggcgcg gcaagaacgc
      121 gccctacgcg ccgctcgcgc cgctcatcga gcgcaaagcg cgtgcgctcg tcgtgctcgg
      181 cgaagacgcg gagcgcatcg agcatgagtt gaaggcgcac gcgcacgtct cgcgcgtgag
      241 agatttggga gaggcggtgc ggcgcgcgtt cgcggcggcg caacccggcg acgtggtgct
      301 gcttgcgccc gcgtgcgcca gttgggacat gttcgagagc ttcgagcacc ggggcagagt
      361 ctttaaggaa gaagtgggaa aaatgatgaa ttcagaacac agaattcaga agccggaaga
      421 gatgcaagtt gcggcttccg ccattctgaa ttctgactcc tgaattctga ctcctgtttt
      481 atggctagaa agctacaagc tgacgagtgg ttgtttgcgg cgacgttggg gctggcgctg
      541 tttggcgtcg tgatggtcta cagcgcgtcg gctgatttag cgtatgtcga aaacggcacg
      601 cagtatcact acgtcaagcg gcaggcgatg tggacggtgg cgggattgct cgccatgatg
      661 gccgcgatgc acttcgatta cgggtggctc agagatcgac gcgtcgtggc gggcatgctc
      721 gccgggacgg tcgtgctgct gatcgcggtc ttcgcgttcc cgcgcatcaa cggcgcgcat
      781 cgctggatca ggtttggcgg cttctctttg cagccttcgg agttgtcgaa actcgctttg
      841 acgatcttcc tcgcgcgctt tctcgaacgg cgcgcgggtg aagagaatgc tctctggaaa
      901 actttcatgc cgtgcgtttt gatgacgggt ctgctcgtca tgctcgtcgc ggcggaacca
      961 gacctcggca cggcgatgat gctcggcatc atctgcttca cactgttgtt cgcggcgggc
     1021 gcgcgcctcc tgtatctggg cgcaatcggg gcggtcggcg tggtcggcat cgtctttatg
     1081 atcgtgctgt tcccgtggcg tctggggcgc gtgacggcgt ttctcgatcc gtgggcagac
     1141 gcgcagggga cgggctttca ggtggtgcag tctttgatcg ccgtcgggtc gggcgggacg
     1201 aacgggctgg gcttcgcgca gggacgacag aagatgctgt ttctgccgtt cgcccattcg
     1261 gatttcatct tcgcggtggt gggcgaggag ttggggttga tcggcgcgtt gacgctcgtt
     1321 ctggtcttcg ggctgttttt gtggcgcggc gtgcgcgcgg cgcggcgtgc gccggatcgt
     1381 ttcgggatgc tgctcggact cggcatcgtg atcggcatcg tctcgcaggc tctgttcaat
     1441 atcagcgtcg tgttgtcgct cgtgccgacg aagggcatcc cgctgccgtt catctcttat
     1501 ggcggttcgt cgatgatgtt gacgctcgtg ggcgtcggcg tgctcttgaa catctcgcag
     1561 caggggacgg cgcagggcga cgccgcgact cgtaaagagt ttgcggcgga gagtgttccg
     1621 gcgacgccac gacgcgagcg cgcgcccgtc acgcagacgt ggcgaccgga aggccggagt
     1681 tgagtttgaa gatgcgcgct ctcttcgccg ccggagggac gggcgggcac atctatcccg
     1741 cgctcgccgt ggcgaaagag ttagtgagcc gccacgagga ttcggcggtg cgattcgtcg
     1801 gcacggcgcg gggcttggag acgcggctcg tgccgcaggc gggcttcaag ttgtcgctca
     1861 ttgaaagcgc ggggctgacg aacatgtctc tcggcgcgcg cctgcgtggc ctgtggctgc
     1921 tgccgaaaag tttcgcggcg gcgaggtctt tgataaggga gtttcgtcct gatgtcgtgg
     1981 tcggcgcggg cggttacgtg acgggttcgg tcttgttgac ggcggcgttg atgcggattc
     2041 cgacgctcgt gatggaatcg aacgccgtgc ccggtttcac gaatcgcagg ctcgcgcggt
     2101 tcgtggatgc ggcgaccgtc tcgtttgaag cgacgttgcc atattttcgc ggcaaggcga
     2161 ttgtgaccgg aaatccagtg cgccgcgagt tctttgacat tccggcaaaa atgcgcgacc
     2221 cttcgcgttt ttctttgctg ctcttcggcg gttcgcaggg cgcgcgtgcg gtgagcaacg
     2281 cgatggtggc cgcgctgccg cacctcgcag cctaccgcga cgtgctgcac atcacgcacc
     2341 agacgggcag actggattac gaggcggtgc gcgcgggtta cgagcaagca ggctggacgg
     2401 agcgcgccga gatcaaggag tacatagacg acatggtggg cgagtttgcc cgcgccgacc
     2461 tgatcgtctc gcgcgccggg gcgacgacgg cggcggagtt gatggccgca ggcaagaccg
     2521 ccatcatggt cccgctcccc ggccaactcg aacaacgacg caacgccgaa gtgttgcagg
     2581 acgcaaacgc cgcacgcatg atcctgcaag agagtctgac gggcgaaact ctggcgaaag
     2641 agatcgccga attgataaac gatcccggag agatcacgcg ccgcgaagag gccagccgca
     2701 aaatggctcg cccgaacgcc gccgcgctga cagttgattt gattgaaaag ctggtgggta
     2761 gacgatgaac gttgaacgcg caaagatgaa cttaaaacta cagacgcctg tttcaccgtt
     2821 catccttcat cgttccgcgt tcatcgtttg attgttatgt ttaatcgcat ccaacacgtc
     2881 catctcgtcg gcatcggcgg catcggcatg tccgggatcg cggaggtctt gtgcaacctc
     2941 ggattccagg tgtcggggtc ggacataaga cgttcgagcg cgacggagcg cctggaaggg
     3001 ttgggcgtga cggtcaaaga gggacaccgc gcggagaatg tgggcgacgc tcaggtcgtg
//
LOCUS       DASWNS010000581         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_359678, whole genome shotgun sequence.
ACCESSION   DASWNS010000581 DASWNS010000000
VERSION     DASWNS010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359678"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1159)
                     /locus_tag="VGB76_20070"
     CDS             complement(59..1159)
                     /locus_tag="VGB76_20070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI/YhbO family deglycase/protease"
                     /protein_id="HEY0101232.1"
                     /translation="MAKPDNTTKRVAILIENHVEDSEFSVPYNALRAAGAEVTVLGSR
                     VNEKYQGKQLKLSMEADRTTTDALAEEFDAVIIPGGMAPDKMRTNMKTVRFVQDAVEQ
                     GKLVAAICHGPQVLIEGELLQGKRATGFRAIRKDMQNAGANFEDAPIVRDGNLLTSRR
                     PGDAPIFTAAILRHLGLEGGLKDVSLPDEGNLAADWWKLAEDWGGSTKSEIADGINTA
                     IRGERYALTAFEHYAEKATAPDLRAAFTEICANKQSHIARLEARLAALGEKESFQAAG
                     SGAWATLKSWLQSSDDVALMRRALGDLQTGVVDSFNLANSFTDPTTAQIFDDISVDIA
                     KHEQRIADLYHARTGTEQADAATPTTGAAVSA"
     gene            1587..2195
                     /locus_tag="VGB76_20075"
     CDS             1587..2195
                     /locus_tag="VGB76_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101233.1"
                     /translation="MPSRRKQSNSGRGNADSSSSGSNSMMDTATETGAGVVNQATEVA
                     SEVAERGSSLGSSLLETLADTMGTVSPTLGDIVRPGGATKRSGGSSGRSSTGRSGTTS
                     TSSAVRSIARQAGKATERAGNAAASEGRATSRASKSASKAITRTANSATASSGGGSAS
                     KKGGAKKGSSKSGGAAKSGGAKKSGSKSGGSAAGRGGSSRKK"
     gene            complement(2568..2640)
                     /locus_tag="VGB76_20080"
     tRNA            complement(2568..2640)
                     /locus_tag="VGB76_20080"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2605..2607),aa:Thr,seq:cgt)
BASE COUNT          549 a          758 c          838 g          509 t
ORIGIN      
        1 ttcaatcacc tctctgcgcc ccttctgtgt cctctctgcg tctctgtcgt gaattctgtt
       61 acgcgctcac ggctgcgccg gtggtcggcg tggccgcgtc ggcctgttcc gttccggtgc
      121 gcgcgtgata gaggtctgcg atgcgttgct cgtgcttggc gatgtcgacg ctgatgtcgt
      181 cgaaaatctg cgcggtcgtc gggtctgtga agctgttggc gagattgaac gaatcgacga
      241 cgccggtttg caggtcgccg agcgcgcgcc gcatgagcgc cacgtcgtcg ctcgattgga
      301 gccaactctt gagcgtcgcc cacgcgccgc tgcccgccgc ctgaaacgat tccttctcgc
      361 cgagcgcggc cagacgcgct tcgagtcgcg cgatgtggct ctgcttgttc gcgcagatct
      421 ccgtgaaggc cgcgcggagg tcgggcgcgg tcgctttctc cgcgtagtgc tcgaaggccg
      481 tcagcgcgta acgctcgccg cgaatcgccg tgttgatgcc gtcagcgatc tcgctcttgg
      541 tcgagccgcc ccaatcctcc gccagcttcc accagtcggc ggcaagattc ccttcatccg
      601 gcagcgagac atctttcaac ccgccttcca gtccgaggtg acgcaggatg gcggcggtga
      661 agatgggcgc gtcgccgggt cggcgcgacg tgaggagatt gccgtcgcgc acgatgggcg
      721 cgtcttcaaa gttcgccccg gcgttctgca tgtccttgcg gatggcgcgg aagcccgtgg
      781 cgcgtttgcc ttgcaggagt tcgccttcga tcaggacttg cggtccgtgg cagatggccg
      841 cgacgagttt gccctgctcg acggcgtcct gcacgaagcg cacggtcttc atgttggtgc
      901 gcatcttgtc gggagccatg ccgccgggga tgatgacggc gtcgaactct tccgcgagcg
      961 catcggtcgt cgtgcggtcg gcctccatag agagcttcaa ctgtttgccc tgatattttt
     1021 cgttgacgcg cgaaccaagt acggtcactt ccgcacccgc tgcccggagg gcgttataag
     1081 gaactgaaaa ctcgctgtct tccacatggt tttcgatcag gatcgcaact cttttcgtcg
     1141 tgttgtccgg cttcgccata taatttttga ctccttcaaa gtatttcaaa tttctgttgg
     1201 acgccgcgcg ctccggggcg tccgcccgcg aatgtgcccg agggttagcc tccggttaaa
     1261 ttggcaaggg ctgtgccagc acgcgcgcgg agtcgaatgc gggcaaaagg cgtccgacgc
     1321 gccgcacgcg cgcgcagaaa gccgtacagg aggaggccgc gaatgttcgt ttacgttact
     1381 tgcggcgggc gtcttcgctc acgcgcggcc tgccgcagat tatttgcgac ctctgcgaca
     1441 ctttatgatt gacggctgcg ccacacttca tgtaaaccac aaggcaactg agaaacgcgc
     1501 aaagtgaaag cgtagggcac aaggttgttt agttcgcctt gagcgcgggt aactcattca
     1561 actcgaaaca aacgggagga gagaagatgc caagcagacg taaacaatct aatagcggga
     1621 gaggcaacgc cgacagcagt agcagcggca gcaacagcat gatggacacg gcgacggaaa
     1681 cgggagcggg cgttgtcaat caggccacgg aagtggcgag cgaagtggcg gaacgcgggt
     1741 cgagcctcgg cagttccttg ctggaaactt tggccgacac gatgggcacc gtgagtccta
     1801 cgctcggcga catcgtgcga ccgggcgggg cgacgaaacg ctcaggcgga tcatctgggc
     1861 gttcgtccac gggacgcagc ggcacaacct caacgtcgag cgcggtgaga agcatcgcgc
     1921 ggcaggcggg caaagcgact gagcgtgcgg gcaacgccgc cgcgagtgaa gggcgtgcca
     1981 cctcacgcgc cagcaagtct gcgagcaaag cgatcacgcg cacggctaat tcggccaccg
     2041 cgtcttcggg cggcggaagc gcgtcgaaaa aaggcggcgc aaagaagggc agttcgaagt
     2101 cgggcggcgc agcaaagtcc ggcggcgcga agaaatccgg cagcaagtct ggcggctcgg
     2161 cagcagggcg cggcggaagc tcccgaaaga agtaagcgcg caacgcgcac gctcgaacgc
     2221 agagagcgcc gcacaattca accttgaacg gatcgcacct accgcgaaac aacgagtgcg
     2281 atccgttcaa ggttagctgt cttgatgcgg ctgagaatat ttaaggagac acatttacag
     2341 cctcttgaac ccgaaacaaa agcgagagca cgtgagtgct atggtacata attcatgggg
     2401 cttgaaaagc gtgcaagaag aagtggctaa gtgtcgtgtc ttgtgtgcta atcatcacca
     2461 gaaacatacg atccggcagt tcggatacaa aaaatggttg gacttagagc gagacgcaaa
     2521 gaaaattgcc gtataactaa gagatagcta tacggcaaaa agaatgaagc caccttggag
     2581 actcgaactc cagaccccct aattacgaat caggtgctct accaactgag ctaaggtggc
     2641 atataagttt gtga
//
LOCUS       DASWNS010000582         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_255670, whole genome shotgun sequence.
ACCESSION   DASWNS010000582 DASWNS010000000
VERSION     DASWNS010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255670"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VGB76_20085"
     CDS             complement(<1..765)
                     /locus_tag="VGB76_20085"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0101234.1"
                     /translation="MKDIYLVGAVRTPIGRFGGALANVPAAELGATAAQESLRRAGLE
                     PGAIQQVIWGAARQAGTGPNLARQIGYRAGAPETVPAYTVNQACGSGLKAIILAAQEI
                     MLGRARAVLAGGVENMSRVPYFAEGARWGLKMGNMELVDGMYRDGFLDPLSGLIMGET
                     AENLARQYEITRDEQDAFALRSQQRAAAAIDSGRFDAELTPVEIRGRRGEPLTLARDE
                     HPRADTTLESLSKLKTPVRNPGTVTAGNASGVNDGAA"
     gene            1017..>2319
                     /locus_tag="VGB76_20090"
     CDS             1017..>2319
                     /locus_tag="VGB76_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101235.1"
                     /translation="MKVLTNPAEREEARCSIASILDARGEWFCARTRDGASVPLGRGA
                     WEIGLTRGGLHFSYRTERGALYVWRVEGWEWTGARLRLSTTRKMGAERAVLELVPRAS
                     VAEGVAQVAAARWAACEKLAALVCAATPNAKVERANLSAGARRAEPGRYARILFSRAG
                     RERFAATGAVVALGAHEVDAFLASALLWWRRSVEKSKRACARKLWLVVRPELRDATAE
                     RLSLLRREVRREIELYETDKGWQNLSPVPLPELQRTFDDAPARLSRTPRPELTETAAQ
                     ILALAPGAIDVIRARHGETLRFRGLPFARVRSLMQRERVWFGVGNAPRLPLDESTWPQ
                     LAKLIDELHAHRHAASVDQRHAFYASAPEAWLESLLRRDITRLDPGLRLAPLYTQFRT
                     APAATRTRHAGARPVDLLAIRRDGRLVVIELKVSEDAALPLQ"
BASE COUNT          365 a          772 c          769 g          413 t
ORIGIN      
        1 ggccgccccg tcgttgaccc cggaagcgtt gccggcggtc accgttccgg ggttgcggac
       61 cggcgtcttg agcttggaga ggctctccag ggtcgtgtcg gcgcgcgggt gctcgtcgcg
      121 tgcgagcgtg agcggttcgc cgcgtcgccc tctgatctca acgggcgtga gttcggcgtc
      181 gaagcggcct gagtcaatcg cggcggcggc gcgctgctgc gagcgcaggg cgaaggcgtc
      241 ctgctcgtcg cgcgtgatct catattgacg cgcgaggttt tcggcggttt cgcccatgat
      301 gaggccggag agcgggtcga ggaaaccgtc tcgatacatg ccatccacga gttccatgtt
      361 gcccatcttc aagccccagc gcgcgccttc cgcgaaatag gggacgcgcg acatgttctc
      421 cacgccgccc gcgaggactg cgcgtgcgcg tccgagcatg atctcctgcg ccgcgaggat
      481 gatcgctttc aaccccgacc cgcacgcttg attcaccgtg taggcgggca cagtctccgg
      541 cgcgcccgcg cgataaccga tctggcgcgc gaggttcgga cccgtgcccg cctgtcgcgc
      601 cgcgccccag atgacctgct gaatcgcccc cggctctaat cctgcgcgcc gcagagattc
      661 ctgcgccgcc gtcgcgccca actccgccgc cggaacattt gcgagcgcgc cgccgaagcg
      721 accgataggc gttcgcaccg ctcccaccaa ataaatatct ttcattgctt cactctctga
      781 attaacattc gccgacagat gacaagataa taacttgtcc ggcaagtaga ttccaaaacg
      841 acgtgcaaac acccgcctcc gcgtcgtgcc tttctttgcg cctccggtct tgacttcttt
      901 gcgtctccgg cgagaactgt atttcacgcc gggcgcgcaa agttaaaacg aaagacgcaa
      961 agcgttcttg ctttgcctct cgactttcca gctatcgctc gcgcagcact tagtcaatga
     1021 aagtcctcac gaatccagcc gagagggaag aggcgcgctg ctctatcgcg tctatcttag
     1081 atgcgcgcgg ggagtggttt tgtgcccgga cgcgggatgg cgcgagcgtg ccgctcggca
     1141 gaggcgcgtg ggagatcggc ctgactcgcg gcgggctgca tttctcttac cggacggagc
     1201 ggggcgcgct ctacgtgtgg cgcgtggagg gttgggaatg gacgggcgcg cgtctgcgct
     1261 tgagcaccac gcgaaagatg ggcgcggagc gtgcggtgtt ggaactcgtt ccgcgcgcct
     1321 ctgtcgcgga gggcgttgcg caggtagcgg cggcgcggtg ggcggcctgc gaaaagttgg
     1381 ccgcgctcgt ctgcgctgcg acgccgaacg cgaaggtcga acgcgccaac ttgagcgcgg
     1441 gcgcgcgacg cgcggagccg ggcagatacg cgcgcatact cttctcacgc gccggacgcg
     1501 aacgcttcgc cgcgacgggt gcggtcgtcg cgctcggcgc gcatgaggtg gacgccttcc
     1561 tcgcgtcggc tttgctctgg tggcggcggt ctgtcgaaaa gtcgaagcgt gcgtgcgcgc
     1621 gaaagttatg gctcgtcgtg cgccctgaac tgagagacgc gaccgccgag cgtctctctc
     1681 tgttgcggcg agaggtgcgg cgcgagatcg aattgtatga gacggacaaa ggctggcaaa
     1741 acctgtcgcc cgtcccgctg cccgaactcc agcgaacttt cgacgacgcc ccggcgcgcc
     1801 tcagccgcac gccgcgcccg gagttgacgg agacggcggc gcagatcctt gcgctcgcgc
     1861 ccggtgcgat tgacgtgata cgcgcgcggc acggcgagac gctcagattt cgcgggctgc
     1921 ccttcgcacg cgtccgcagc ctgatgcagc gcgagcgcgt ctggttcggc gtggggaacg
     1981 cgccgcgcct tccgctcgac gaaagcacct ggccgcaact cgcaaaactc atcgacgaac
     2041 tgcacgcgca caggcacgcc gcctccgtcg atcaacgtca tgccttctac gcgtctgcgc
     2101 cggaagcgtg gcttgagtcg ttgctgcggc gcgacatcac gcggctcgat ccgggtttgc
     2161 gtctcgcgcc gctctacacg cagtttcgca ccgccccggc agcgacacgc acgcgccacg
     2221 caggcgcgcg tcccgtagac ctgctcgcca tcaggcgcga cgggcggctc gtcgtcatcg
     2281 agttgaaggt ttcggaagac gccgccctcc ccctgcaag
//
LOCUS       DASWNS010000583         4552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_231318, whole genome shotgun sequence.
ACCESSION   DASWNS010000583 DASWNS010000000
VERSION     DASWNS010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231318"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..4056
                     /locus_tag="VGB76_20095"
     CDS             226..4056
                     /locus_tag="VGB76_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101236.1"
                     /translation="MIVYKLLLASLGLFALFNYLIIPEVKRKWDANRTLWKVSKVGLL
                     SMLDFARTTFLLAAVTYVFVLLVAWVLSLTGASTAQGLAGVVARMQGYRSGLSTFEDY
                     WGVWVFGLLLVALCVLAYRRGKRHHAEQFQATVNREYERLRLEREEGVWEDLPPTPEM
                     QAIEERLRWGVGVLDQLEQSQAPRDAEFETNRESLRLFLQTLEQRWHSLDWLRRMNLT
                     WEDDDDAATAPQGKGWRARAQTFFISKGMLKSLKAGSRTLGHVGMVLLLLSLIGVNAS
                     SLNNAFAARIVRLDELQVKASEQEARESFARVQAEQRDGGKQELDKEDEDALDDLSRQ
                     FEDAFASSGAWRAVQTPRTVVSEMRRLNVRGRILANVRRAAPASADVGASSASAATEA
                     AQSSPLERESIELYERTSSKPGPQTKVGERTRVNLRQEVAGQSSKLWEGVKTKWRAHV
                     AEFGRAARMADIGDFMVGEIAGNALEGFRGEASSEMGKLFQQMTNEGAADSIKSMYET
                     QFRQFVVDLAGPDSMDEALRRVRTEVPGRKAYTLTNAARLRETTARLPDSEKLVATLR
                     ERPPTLRENANPAKIQATTDKLKSVYKSHPNAGAGGAARPVEASASYEDYFPGHIDSE
                     RNTVQGKLLAELHPETVRPPGPEGGGGSGGATKGGNPEPGSGGGAGGGGGGGGGGGGG
                     GSSSGKSGGGGGSSGGGVGSGSGSAGGGGAGGVEYNHARSGGGSHARTQGGGRTSFAR
                     ARSFSMLRGFARVGGVLIGHPLAELQSDARADFRDFGYELDGNEVRLRLRRADGREFV
                     LGPYRKAIVHQALSYVADGRSTAVTMTSATPLEELKVLLHPSFIDTPLGCRVIDIDRF
                     VDEFSGCRDPRGQCTDPRTLAVTRTQEQVILYQFAWLSRQLSLLGAGGSSAESGTPDA
                     FEIKRQRDELLPYAGRALKNAAALNDPKQSLLASKPMFFDPELVAQMVECVRGNSTAA
                     ESFATCVEDRAGQRSVDESTRRKWMTEPPSPVNWSGVREVAFSIDPDLRFLAPPSGDE
                     TATRLWPFEFMLQTAFPEPPTFLPSGEDPEGFTDDAPWDFQELKPHIAERVWQGLRGS
                     ERHMSVFRDVRELTVLQRLFRVMLEGGLGERLEIDGLPDLAEATGGSYPYARTPRWNA
                     SPGAIEIGYARRLFTGLLALSTQSADRAVGSSEWFTEVLGDMRSAYERILQNKHPELI
                     SSEEWQNAYTLNRGRLAAMQRCQLTSDEMACGLNRALSQLGEGVHFARNLRFALGVSE
                     DDRTGRAAHVCPPL"
     gene            complement(4124..>4552)
                     /locus_tag="VGB76_20100"
     CDS             complement(4124..>4552)
                     /locus_tag="VGB76_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEY0101237.1"
                     /translation="RTLFFDFMPLELGTVRGFKTRFHLYTVPGQVFYDASRKLILKGV
                     DGVVFVADSQEERMDANIEALYNLEENLKANGYELTKLPYVLQLNKRDLPNVIPAEEL
                     VSELRRKDEAVHEAVAYKGVGVFDTLKSVAKQVLTELRKG"
BASE COUNT          860 a         1382 c         1523 g          787 t
ORIGIN      
        1 gttgcgaaga caacggcctc tacgtgccgg tcgcaaactt cgacgcctgt ggctccagag
       61 ctttcgccgt cagtgtccgc gccccgcgaa cgataaggca cacctgttga ctcacgttac
      121 cttaaacgga aacggtcgcc cgcactgctg attccaccac cctgaggggc tagcctcctg
      181 cgacttctca agtcggctac ctccttcctc caaaggagtg catccatgat cgtctataaa
      241 cttcttctcg ccagcctcgg cctcttcgcc ctcttcaact atctcatcat cccggaagtt
      301 aaacgcaagt gggacgccaa ccgcacgctc tggaaggtga gcaaggtcgg cctcctctcg
      361 atgctggact tcgcgcgcac cactttcctg ctcgccgccg tcacctacgt cttcgtgctg
      421 ctcgtcgcgt gggtgctttc gttgacggga gcttcgacgg cgcaaggtct ggccggcgtc
      481 gtcgcgcgca tgcagggcta ccgcagcgga ctctcgacgt ttgaggatta ctggggcgtg
      541 tgggtcttcg ggttgttgct cgtcgccctc tgcgtgcttg cataccgcag gggcaaacgg
      601 catcacgcag agcagtttca ggcgaccgtg aatcgagagt atgagcggct gcggcttgaa
      661 cgcgaggaag gcgtctggga agatttgccg ccgacgccgg agatgcaggc gattgaagag
      721 cgtctccggt ggggcgtcgg cgtgctcgat caactcgaac agagccaagc cccgcgcgac
      781 gccgagtttg agacgaacag agaatccctg cgcctgtttc tacagacgtt ggagcaacgc
      841 tggcactcgc tcgattggct gcgccgcatg aacctgacct gggaggatga cgacgatgcc
      901 gcgaccgcgc cgcaaggcaa aggctggcgc gcgcgggcgc agactttctt tatcagcaag
      961 ggaatgttga agagcctgaa agccgggtcg cgcacgctcg gccacgtcgg gatggtgctc
     1021 ctgctgctgt cgctcatcgg cgtcaacgcg tcgtcgctca acaacgcctt cgcggcgcgc
     1081 atcgtgcgtc tggacgagtt gcaggttaag gccagcgagc aggaggcgcg cgagagcttt
     1141 gcgcgcgtgc aggccgagca gcgcgacggc ggcaagcagg aactcgacaa ggaagacgag
     1201 gacgcgctcg acgatctctc gcgtcaattc gaggacgcgt tcgcctcttc gggcgcatgg
     1261 cgcgccgtgc agacgccgcg cacggtcgtc tccgagatgc gacgcctgaa cgtgcgcggg
     1321 cgcatcctcg ccaacgttag acgtgccgcc ccggcgagcg cggacgtggg cgcatcttcc
     1381 gcgtcggctg cgacggaagc cgcgcagtca agcccgctcg aacgcgaaag catagaactc
     1441 tacgagcgca cgagcagcaa gccgggacca caaacgaaag tcggcgagcg cacgcgcgtc
     1501 aacctgcgtc aggaagtggc cgggcagtcg agcaagctgt gggagggcgt caagaccaag
     1561 tggcgcgcac acgtcgcaga gtttggccgc gcggcgcgca tggctgacat cggcgatttc
     1621 atggtcggcg agatcgcagg caacgcgctc gaaggctttc gcggcgaggc gagcagcgag
     1681 atggggaaac tgtttcagca aatgacgaac gagggcgcag ccgactcgat caagagcatg
     1741 tatgagacac agttccggca gttcgtcgtc gatctggcag gtcccgactc gatggatgag
     1801 gcgttgcggc gcgtgcgcac ggaggtgccg gggcgaaagg cgtacacgct gactaacgcc
     1861 gcgcgcctgc gcgagacgac ggcgaggttg cccgacagcg aaaaattggt ggcgactctc
     1921 agagagcgtc cgccgacgct gcgcgagaac gcgaacccgg ccaagattca ggccaccaca
     1981 gacaaactga aaagcgtgta caagtcgcac ccgaacgcgg gagcgggcgg cgcggctcgc
     2041 ccggtcgagg catccgccag ttacgaagac tacttccccg gccacataga ctcggagcgc
     2101 aacaccgtgc agggcaaact tctggccgaa ctgcacccgg agacagtcag gccgccggga
     2161 cctgaaggcg gcggcggaag cggcggcgcg acgaagggcg gcaaccccga acccggaagc
     2221 ggtggcggcg cgggtggtgg cggaggtggc ggcggtggtg gcggcggagg cggtagcagc
     2281 agcggcaaga gtggtggcgg gggaggaagc agtggcggcg gtgtgggaag tggtagtgga
     2341 agtgccggag gcggcggcgc ggggggcgtt gagtataacc atgccagaag cgggggcggg
     2401 tcgcacgcac gcacgcaggg cggcgggaga acgtcctttg cgcgagcgcg cagtttttcg
     2461 atgctgcgcg gcttcgcgcg tgtgggcggc gtgctcatcg gccacccgct cgcagagtta
     2521 cagagcgatg ctcgcgccga ctttagagat ttcggctacg aactcgacgg caatgaggtg
     2581 cgtctacgtc tgcggcgcgc ggatgggcgc gagtttgtgc tcggccccta ccgcaaggcc
     2641 atcgtgcatc aagcgctctc ttatgtcgcc gacgggcgca gcaccgccgt cacgatgacg
     2701 agcgcaacgc ctttggagga gttgaaagtg ctcctgcacc ccagtttcat agacaccccg
     2761 ctcggttgcc gcgtcattga catagaccgc ttcgtcgatg agttctccgg ctgccgcgac
     2821 ccacgcgggc agtgtacaga cccgcgaact ctggccgtga ctcgaactca ggaacaggtc
     2881 atcctctacc agttcgcgtg gctgagcagg caactgtcgc tgctcggcgc gggcggctct
     2941 tctgccgaga gcgggacgcc ggatgccttc gagatcaaac ggcagcgcga cgaactgctc
     3001 ccctacgccg ggcgtgccct gaagaacgcc gcagcgctca acgaccccaa acaatcgctg
     3061 ctcgccagca aaccgatgtt cttcgacccc gaactcgtcg cacagatggt cgaatgcgtg
     3121 cgcggcaatt cgacggcggc agagagcttc gccacctgcg tcgaagatag agcgggacag
     3181 cgcagcgtag atgaatcaac gcgccggaag tggatgaccg agccgccctc gccggtcaac
     3241 tggagcggcg tgcgcgaagt ggcgttcagc attgatcccg atctcaggtt cctcgcgccg
     3301 ccctccggcg acgagacggc gaccagacta tggccgttcg agttcatgct ccagacggcc
     3361 ttcccggagc cgcccacgtt tctcccctcc ggcgaagacc ccgaaggctt caccgacgac
     3421 gcgccctggg attttcaaga gcttaaaccg cacatcgccg agcgtgtgtg gcagggcttg
     3481 cgcggcagcg agcggcacat gagtgtcttt cgagacgtgc gtgagttgac cgtcctccaa
     3541 aggctgttcc gggtcatgct ggaaggcggc ttaggcgaac gccttgagat agacggcttg
     3601 cccgacctgg cggaagcgac cggcgggagt tacccctacg cgcgcacgcc gcgttggaac
     3661 gcaagtcccg gtgcgattga gataggctac gcccgacgcc tctttaccgg cctgctcgca
     3721 ctctcgacac agtcggctga ccgcgccgtg ggcagctccg agtggttcac cgaagttctg
     3781 ggcgacatgc gctcggctta cgaacgcatc ctccagaata aacatccgga gttaatttcg
     3841 tccgaggagt ggcagaacgc gtacacgctc aaccgtggac ggctggcggc tatgcagaga
     3901 tgccagttga ccagcgacga aatggcgtgc ggcctgaacc gcgcgctgtc gcaactcggc
     3961 gaaggagtcc acttcgcgcg caacctgcgc ttcgcgctcg gcgtctccga agatgatcgc
     4021 acgggtcgcg ccgcgcacgt ctgcccgccg ctttgatgga aagagcggga ggtggcctgt
     4081 ttttgattgc tttaattatt tgagaggaga gagagagacg gctctaacct ttgcgcagtt
     4141 cggtgagcac ctgcttggcg actgacttga gcgtgtcgaa gacgccgacg cccttgtaag
     4201 cgacggcttc atgtaccgct tcgtctttgc gtcgcagttc gctcacgagt tcttcggcgg
     4261 ggataacgtt cggcaggtcg cgcttgttga gttgcaggac gtaggggagt ttcgtcaact
     4321 cgtagccgtt ggctttcaaa ttctcttcca gattgtagag agcttcgatg ttggcgtcca
     4381 tgcgctcttc ctgcgagtct gcgacgaaga ccacgccgtc gacgcccttg aggatcagtt
     4441 tgcgcgaggc gtcgtagaag acctgaccgg ggactgtgta gagatgaaag cgggtcttga
     4501 agccgcgcac cgtgccgagt tcgagcggca tgaagtcgaa gaagagcgtg cg
//
LOCUS       DASWNS010000584         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_48352, whole genome shotgun sequence.
ACCESSION   DASWNS010000584 DASWNS010000000
VERSION     DASWNS010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48352"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VGB76_20105"
     CDS             complement(<1..598)
                     /locus_tag="VGB76_20105"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0101238.1"
                     /translation="MSYTNLFRKKSVVQIKRDAELGYSDAHGELAKDAEPHLRKTLGV
                     LDLTSLGIAAIIGAGIFAMIGNAANSGGPAVIFLFMFTAMACGFSALCYAEFASKIPV
                     AGSAYTYAYASFGELVAWIIGWDLLMEYAIGNIAVAISWSDYFTGLLSGYGINVPAYL
                     TMDYLTASRGYTAINDLVSQGNTLEAVRAMPDITNNMKE"
     gene            777..1559
                     /locus_tag="VGB76_20110"
     CDS             777..1559
                     /locus_tag="VGB76_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101239.1"
                     /translation="MPESLRDKARALHENLDTAYVNLAALLRYLQQRDFNGRVHVELD
                     EYDGDIYLAAREAVRARERDHVTGREDEGQAALQRILVRASEPGGRVSVYEGAEHAMG
                     DAAGLRTTTADEAATTGTRLTQEERDRRNVLQLSGEVIAAVERGVAAASNENFAAHFQ
                     AARHEIADDYPFLLSLGNCVEYENGEARLKRGMEIEKFVVGACEALRRVVERVAATGE
                     RARGVRREVARELAALSERRPRALTRFGFTPQLERIAGLRWT"
BASE COUNT          335 a          467 c          564 g          295 t
ORIGIN      
        1 cctctttcat gttgttcgtg atgtcgggca tcgcgcgcac ggcttcgagc gtgttgccct
       61 gcgagacgag atcgttgatg gctgtgtagc cgcgcgaggc cgtcagatag tccatcgtga
      121 gataggcggg cacgttgatg ccgtagccgg agagcagccc cgtgaaatag tcgctccagg
      181 agatggcgac ggcgatgttg ccgatggcgt actccatgag caagtcccaa ccgatgatcc
      241 acgcgacgag ttcgccgaac gaagcgtagg cgtaggtgta ggccgaaccg gccaccggaa
      301 tcttcgaggc aaactcggca tagcagagcg cggagaaacc gcaggccatc gccgtgaaca
      361 tgaagagaaa gatcacggca ggaccgccgc tgttggccgc gttgccgatc atggcgaaga
      421 tgcccgcgcc gatgatggcg gcgatgccga gcgaggtgag atcaagcacg ccgagcgttt
      481 tgcgcaagtg cggttcggcg tctttggcga gttcgccgtg agcgtcgctg tagccgagtt
      541 cggcgtcgcg cttgatctgc acgacgctct ttttgcgaaa caaatttgtg tagctcatcg
      601 agttttccgg ttgagaaacg cgtggttgct agagttagca ggactggaaa cagcgtcgcg
      661 gagtctagca taaaatgatg aagtaggaat tctgtatttt gtattctgct tcaccgtaca
      721 tcgtttatgt tagacttctc cggccaaatc ttgatgcgcg gtttctagtg cctgacatgc
      781 cggaaagtct tcgcgacaaa gctcgcgccc tgcacgaaaa tctcgacaca gcttacgtca
      841 atctggcggc gctcctgcgc tacctccaac aacgcgactt caacggacgc gtccacgtcg
      901 aactcgacga gtacgacggc gacatctacc tggcggcgcg cgaagcggtg cgtgcgcgcg
      961 aaagagatca tgtgacgggg cgcgaggacg agggacaggc ggccttgcag cgcatcctcg
     1021 tgcgcgcgag cgaaccgggc gggcgcgtca gcgtctacga aggcgcagag cacgcgatgg
     1081 gcgacgccgc cgggttgcgc acgacgacgg cggacgaagc ggcgaccacg ggaacgcgac
     1141 tgacgcagga ggagcgcgac cgtcgcaacg tcttgcagtt gagcggcgag gtgatcgcgg
     1201 cggtggagcg gggcgtggcg gcagcgtcga acgaaaactt cgccgcacac tttcaggcgg
     1261 cgcggcacga gatcgcggac gactacccgt ttctgctctc gctcggcaat tgcgtggaat
     1321 atgagaacgg ggaagcgcgc ctgaagcgcg ggatggagat cgagaaattt gtcgtcggcg
     1381 cgtgcgaggc tctgcggcgg gtggtggaac gcgtggccgc gacgggcgaa cgcgcacgcg
     1441 gagtacgccg cgaagttgca cgcgaactgg ccgcgctctc cgaacgacgc ccgcgcgccc
     1501 tcacgcgttt cggcttcacc ccgcaactcg aacgaatcgc gggcttgcgt tggacgtgaa
     1561 aggagcgtct agcgtttagc tgtgggccat agagattgcc ccgccggtcg tcagccgaaa
     1621 gcaatttcaa agcgtcgaag cggcgagagt caacggcctc c
//
LOCUS       DASWNS010000585        10394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_204443, whole genome shotgun sequence.
ACCESSION   DASWNS010000585 DASWNS010000000
VERSION     DASWNS010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10394
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204443"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VGB76_20115"
     CDS             <1..277
                     /locus_tag="VGB76_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101240.1"
                     /translation="CLITYFLLNEESTPMLEGGRSILNFVEQSEVYRTINRSRPEDAV
                     AYDEKFVRKLYEKYGLTVSEPVYYGSWCGRENFLSLQDMVLAYKAQR"
     gene            complement(310..933)
                     /locus_tag="VGB76_20120"
     CDS             complement(310..933)
                     /locus_tag="VGB76_20120"
                     /inference="COORDINATES: protein motif:HMM:NF016850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HEY0101241.1"
                     /translation="MHSISEQFHELYYESRVWRNTFWLGVETLKCPLDLWIYQELLQE
                     VRPRYIIETGTAAGGSALFLASVCDLLGTGEIITVDIEERPARPAHARIEYLKGSSVA
                     VDTVRAIKERVGVAAPVLAILDSDHSQQHVSQELQIYGAMVTEGSYLIVEDTNVNGHP
                     VLQHHGAGPMEAVESFLRETPQFTPDREREKFFLTFNPKGYLRKIKG"
     gene            complement(942..1739)
                     /locus_tag="VGB76_20125"
     CDS             complement(942..1739)
                     /locus_tag="VGB76_20125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEY0101242.1"
                     /translation="MNKDSAFLQTRRELVDAGMCAQNEARRLYLDLMKRALLNLIYGE
                     HEQRVKGFDERSRIEGREWPAMAHTMLGWARLENLQHCIEDILARGVPGDLIETGVWR
                     GGAIIFMRAVLKAYEVTDRCVWGADSFRGLPPPDAAQYPADEGDICYTCPELAVTLDE
                     VRSNFEKYGLLDEQCRFLPGWFRDTLPTVRAERFALIRLDGDMYESTYVALANLYPKL
                     SRGGYLIVDDYGALPSCRQAVHDFRDAHGIREEIIPIDWTGIFWQRD"
     gene            complement(1776..3029)
                     /locus_tag="VGB76_20130"
     CDS             complement(1776..3029)
                     /locus_tag="VGB76_20130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0101243.1"
                     /translation="MKPIIQVENLSKLYYLGRHAQASAAAGGALLNAATAPLRMLARR
                     RRLSEARPFWALKDLSFEVGRGEIVGIIGRNGAGKSTLLKILSRVTKPTSGMADIYGR
                     VGCLLEVGTGFHPDLSGRENIYLNGAILGMKREEIARKFDEIVDFAELGDFLDTPVKH
                     YSSGMYLRLAFAVAAHLEPEILLLDEILAVGDAAFQKKCLGKMQEVSRAGRTILFVSH
                     NMAAVQQMCERVLLLDSGRLREEGTPGAVIDHYLNDASQEEHGDFDLTNHSARLPNCA
                     PVIRRLRLSAEGSPSSTRFHPGQEMTVELLLEPAAVIREPRLAISIEDHTGQRITTVA
                     SYFQNDALPDIERRCRVRCALPVRLGSGRYLLSVSVANKYQGMIDGLHHAAWFEVVWR
                     NNFDNGEAYDPVYGATLTPSTWELS"
     gene            complement(3074..3988)
                     /locus_tag="VGB76_20135"
     CDS             complement(3074..3988)
                     /locus_tag="VGB76_20135"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0101244.1"
                     /translation="MSIREKFDPLESVEAQLSDRGAQTLASLADLPERPVIKIRPADT
                     RLRIDLGEVWAHRELFYLLVWRDLKVRYKQTFLGSTWTILQPLLLALVFTVFLGRLMR
                     VPSDGIPYAAFAYVGLVAWSFFANACMGSSYSLISNAHLIKKVYFPRLLIPCAAVAVR
                     LIDFLFASLILMPMLLYQGITPGWSLLIFPLFILELTLLSLGFGLLLATLNLKYRDVG
                     TLLPVALQLWMFVSPVVYPLTLVPEQWRRVYALNPIVGIIEGMRGAVFGREPDFYATA
                     VSVGITVPLLIFSVYCFRRAEEKFADVV"
     gene            4411..5223
                     /locus_tag="VGB76_20140"
     CDS             4411..5223
                     /locus_tag="VGB76_20140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0101245.1"
                     /translation="MFLVDIARPALLVELGTYTGVSFSAFCQAVEGLGLETRCHAVDT
                     WKGDDQTGYYGGETLADLKAHIETHYKGFAQLIESTFDQALHRFEDGTIDLLHIDGHH
                     AYESVRHDFESWLPKMSARGVVLLHDTNVREPDFGVWKLWEELKVRYPHFEFIHEHGL
                     GVLATGTEMSDGLRLLTGTSPEEAAVWRKFFHQLGHRFRVQLDKENEIHLLTAQLEAH
                     ALQLQTVSHELRNILNSRTWRSLGRYMWLKNFLQMSAARLRQNLRTTRKDAQ"
     gene            5347..5607
                     /locus_tag="VGB76_20145"
     CDS             5347..5607
                     /locus_tag="VGB76_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101246.1"
                     /translation="MPVSEMMTPERWRQVETVFQTALDITREHRERFLAEACAGDEVL
                     RREVEHMIEGYEAADEFLELERHYSFLRAISGSTRVARRAGM"
     gene            complement(5547..8186)
                     /locus_tag="VGB76_20150"
     CDS             complement(5547..8186)
                     /locus_tag="VGB76_20150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0101247.1"
                     /translation="MWLKGLYRRVRALICKEAIHNEIDEEMRFHIDMREEENVRQGMS
                     PEEARRDAERRFGRFTRVKEQGYEVRGGRWLESLWQDCRYGTRMLWKNFGFTTVAVLV
                     LALGIGANTSLFSVVNAVLLRPLPFPDAERIIWFDGVHQARGITASNLSPADYSDWEK
                     QASDFESMSAYVTGSANLSDEVGEPERVPRVSVTAGFFPTLGASPALGRALLPEDESP
                     GGEDVAILSHALWQRRFGASPGVVGTRLLVNGTPVTVVGVMPAGFDFPRASQMWTPLK
                     PEKSAQKRDNRYLNVLARLKPDVTLEEAETQLGIINSRLGQQYPETNGGWSVRLTPLH
                     ERTVRDVSTSLWLLLGAVGFVLLIACANVTNLLLARAAGRRREIALRTALGAGRLRVI
                     RQLLTESLMLATLGGAVGLVLSFFLMDLLVSISPANLPRLNEVSMDWRVLGFTAGAVC
                     VVGLLFGIAPALQASKTDLNEVLKEGSFGSSGGLGRNRVRAALVISEVALSLLLLVGA
                     GLLIRSFLLLHAVDAGFDPEKVLTMRVSLPSTRYSDPQQRVNFFRQLTERVEALPGVE
                     AAGAAISLPLGGTTLSVARGFVREGNPLTTEHAFDASYNVTTPGYIKTMRIPLKSGRV
                     FTDRDAADAPPVAIINESLARRAFPDIDPVGKRLTAWRDEEFAREIVGVVADVKMGQM
                     EGEDRPQVYVPQAQNATWGALSLAVRTSVEPESLAGAVREAVLAIDKSQPVYDIRTME
                     DVRSASVANRRLIVLLFAGFATLAFLLAVVGIYGVLSYAVTQRTHEIGIRLALGAQRA
                     DVLRLVVGQGMRLVVLGVAVGMVGAFLVTRLLKSLLFGVSAADPVTFAVVALVLSVVG
                     LLACYIPARRATRVDPLIALRNE"
     gene            complement(8203..8535)
                     /locus_tag="VGB76_20155"
     CDS             complement(8203..8535)
                     /locus_tag="VGB76_20155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0101248.1"
                     /translation="MAKQKADLLQGTLDMLILKALSVGALHGYGVGQQIEQMAEDMLK
                     VEEGSLYPALYRLEERGWIKSEWGKSDNNRRARFYTLTRTGRRQLGVEEEHWRLMVLA
                     IDKVMQAA"
     gene            complement(8641..8811)
                     /locus_tag="VGB76_20160"
     CDS             complement(8641..8811)
                     /locus_tag="VGB76_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101249.1"
                     /translation="MKDSEVDQYVHRDPARLARLRAPKPYQTESVEALSDEEVRALLG
                     TVRRRAHCDQVI"
     gene            complement(9127..9408)
                     /locus_tag="VGB76_20165"
     CDS             complement(9127..9408)
                     /locus_tag="VGB76_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101250.1"
                     /translation="MWEEQVIQRIRLQLLALVLAFALTWYAMNPAPGPQWHVGRSEEQ
                     PDEKRREEKSLGPMGYELCGTEGVVMKSTVDPAAQDIEDLEWPEVIDIR"
     gene            9591..10370
                     /locus_tag="VGB76_20170"
     CDS             9591..10370
                     /locus_tag="VGB76_20170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101251.1"
                     /translation="MRATRRRQRRPDPLASPDAVRNVINQLRDALPDIIPQKDKDLVR
                     MLRAARHAQRYPATDTKRGRPGRWRREDLLKIATRLGDILERETSSHISVASFVDHYL
                     RLLEFPADVSEALASGQINLFEAEQLARITPERLQVTPSQARRTRTDLLSTHLQTKAS
                     GTQLRQRVNELLKQPEAATQSDESFLEYLEDLEDFDPYDPTHLFWEQIKQLGFAFREI
                     RREDVLDEEIEELLKASEPIMAVLSRIQRRKEQRGMTKLQL"
BASE COUNT         2266 a         3147 c         2856 g         2125 t
ORIGIN      
        1 atgcctgatc acctatttcc tcctgaacga agagtcaacc ccgatgctcg aaggggggcg
       61 gagcattctc aattttgtgg agcagtccga agtctaccgc acgatcaacc ggagcaggcc
      121 ggaggacgcc gtcgcctacg atgagaagtt cgtcaggaag ttgtatgaga agtatgggct
      181 gacggtgagc gaacccgtct actacggttc gtggtgcggg agagaaaatt tcctgagctt
      241 gcaggacatg gtgctggcct ataaggctca acgataaaag ctatgcccgc ggcgacatcc
      301 acgtcgccgc tatcctttta tctttcggag ataacctttc gggttaaaag tcaggaagaa
      361 cttttcccgt tcgcggtctg gagtgaactg tggcgtctcc cgcaaaaacg actcgacggc
      421 ttccataggt cccgccccgt ggtgctggag caccgggtgg ccgttgacgt tcgtatcctc
      481 gacgatcaga tagctgcctt ccgtcaccat cgccccatag atttgcaact cctgagagac
      541 atgctgctgg ctgtgatccg aatcgagaat cgccagcacg ggggcggcca cccccactct
      601 ctccttaatc gcgcggacgg tatcgacggc gacggacgag cctttcagat attctatcct
      661 cgcgtgcgcg gggcgcgcgg gacgttcttc gatgtctacg gtgatgatct ctcccgtgcc
      721 cagcaggtcg cagacggagg ccagaaagag cgcgctgccg cccgccgccg tccccgtctc
      781 gatgatgtaa cgcgggcgca cctcttgaag caactcctga taaatccata aatcgagcgg
      841 gcatttcagc gtctcgacgc cgagccagaa ggtgttccgc cagacgcgcg attcgtaata
      901 aagctcgtgg aactgctcac tgatcgagtg catgtttcaa ctcagtcgcg ttgccagaaa
      961 atcccggtcc aatcaatcgg aatgatctct tccctgatgc cgtgcgcgtc gcggaagtcg
     1021 tgtacggcct gacgacacga gggaagcgcg ccgtagtcgt ccacgatcag gtagccgccc
     1081 cttgacagct tggggtagag gttcgccagc gcgacataag ttgattcata catatcgccg
     1141 tcgaggcgaa tcaaagcgaa ccgctctgcg cggacggtgg gcagggtgtc gcggaaccag
     1201 ccgggcagga agcgacactg ctcatccagc agtccatact tctcaaaatt cgagcgcacc
     1261 tcgtcgagcg tgacggcgag ttccgggcac gtataacaaa tgtctccctc gtccgccgga
     1321 tattgagcgg catcgggtgg aggcagccct cgaaaagagt ccgcgcccca gacgcagcgg
     1381 tctgtcacct cgtaggcttt cagaaccgcg cgcatgaaga tgatcgcgcc gccgcgccac
     1441 acgcccgtct cgatcagatc gcccggcacc cctcgcgcca gaatatcctc gatgcagtgc
     1501 tgtagatttt caagacgcgc ccaaccgagc atcgtatgcg ccattgcagg ccattcgcgc
     1561 ccttcaatgc gcgaccgctc atcgaacccc ttgacccgct gttcatgctc gccgtagatc
     1621 agattgagta aggcgcgctt catcaaatcc agatagaggc ggcgcgcttc gttctgcgca
     1681 cacatgcctg cgtccactaa ttctcgcctg gtctggagaa atgcactgtc cttgttcaca
     1741 gactgctcca tcaaatcaac tgagttcccg gcaaatcaac tgagttccca ggtggagggc
     1801 gtcagcgtcg ccccgtagac agggtcgtaa gcctcgccgt tatcaaaatt attgcgccag
     1861 acaacttcaa accaggccgc gtggtgaaga ccgtcaatca taccctgata tttattggcg
     1921 acgctgacgc tcaagagata ccttccgctt ccgagtcgta cgggcagcgc gcaacggacg
     1981 cggcagcgtc tttctatatc cggcagcgcg tcgttttgaa aataggaggc gaccgtggta
     2041 atgcgttgcc ctgtgtggtc ttctatggag atggcgagac gcggttccct gattacggcg
     2101 gcaggctcaa gcagcagttc gacggtcatc tcctgaccgg gatgaaagcg tgttgacgaa
     2161 gggctgcctt cggcggagag cctcaggcga cgaatgacgg gggcgcagtt gggcagtcgc
     2221 gccgaatggt tcgtcagatc gaaatcgcca tgctcttcct gtgacgcgtc gttgagatag
     2281 tggtcgatga ccgcgccggg cgtgccctcc tcacgcagcc tcccggagtc gagcagcaac
     2341 acacgctcac acatctgctg gacggcggcc atgttatgac tgacgaacag aatcgttcgc
     2401 cccgcgcggc tcacctcctg cattttgccc agacacttct tctggaaagc cgcatctccc
     2461 acggccagaa tttcatccaa cagcagaatc tccggttcga gatgcgccgc gacggcgaaa
     2521 gcgagtcgca ggtacatgcc gctcgaatag tgtttgacgg gcgtgtccag aaaatctccc
     2581 aactcggcaa aatccactat ctcgtcaaac ttgcgcgcga tctcctcgcg cttcatgccg
     2641 agtatcgcac cgttgagata gatattctca cgtcccgaaa ggtctggatg aaagcccgtc
     2701 ccgacttcga gcagacaccc gacgcgtccg tagatgtcgg ccatgcccga tgtcggtttc
     2761 gtgacgcgag agagaatctt gagcagcgtt gacttaccag ccccgttgcg accgatgatg
     2821 ccgacgatct cgccccgccc gacctcaaag ctcaaatcct tcaaagccca aaatggcctg
     2881 gcctccgaca gcctccggcg tcgcgctaac attctaagcg gggcggtggc ggcattcagt
     2941 aaagcgccgc ccgccgccgc agacgcctgc gcgtgccgtc cgagataata gagcttactt
     3001 aaattctcaa cctgaataat cggcttcatc aaacaccaat ttgtgtctct caagctggcc
     3061 gctgaaattt gcgctaaacc acgtcggcga atttttcttc cgcccggcgg aaacagtaga
     3121 ccgagaagat gagtagcggc acggtgatcc cgaccgagac ggcggtggca tagaaatccg
     3181 gttcccgccc gaagacagcc ccccgcatgc cttcgataat acccactatc ggattgagtg
     3241 cgtaaaccct gcgccactgt tcgggcacga gggttaacgg gtagacgacc ggcgagacga
     3301 acatccagag ttggagcgcc accggcaaga gcgtacccac atctcgatac ttgaggttga
     3361 gagtcgccaa caggaggccg aagccgagtg acagcagcgt cagttccaga atgaacagcg
     3421 ggaagatgag caggctccag cccggggtga taccctggta aagcagcatc ggcatcagaa
     3481 tgagagaggc aaagaggaag tctatcaacc ggacggcgac ggcggcgcac gggatgagca
     3541 ggcgcgggaa atagactttc ttgatgaggt gcgcgttgct tatcaggcta tagctactgc
     3601 ccatgcaggc gtttgcgaaa aaactccacg ccaccagacc gacataggca aaagccgcgt
     3661 aaggaatgcc atccgagggc acgcgcatca gcctgccgag aaagaccgtg aagaccagag
     3721 ccagcaaaag cggctgcaaa atggtccaag tggaaccgag gaaagtctgc ttgtagcgca
     3781 ccttgaggtc gcgccaaacg agtagataga acaactcgcg atgcgcccac acctctccca
     3841 agtcgatcct gagccgggtg tcggcaggtc tgatcttgat cacaggccgc tccggcaaat
     3901 cagccagcga agcgagggtc tgcgcccctc tgtcgcttaa ttgggcttcg acggattcaa
     3961 gcggatcaaa tttttcgcga atagacatgc gcgccaaatt attcgtaaat tctacattcg
     4021 ggctgctgta gaagtttgtc gttgaatgcg acgaatgcga cgacggcggg agttaacgaa
     4081 tcgccggtcg attccctctc gcatgcgaac gcgcagttca acatctatga tgttatcttg
     4141 tgaatgaaag tttgacaggc tttctatctg gctgacgagt ctaggggagc gtctcgcggg
     4201 cgtcaagaga gaggggagag gcgatcctgt cggggcatgc tgctctaaag ctcgtttttg
     4261 aattttgaga ttcagacttc cctgcttaat cgtgcgtggg aatctctgtg ggaacatatg
     4321 cctggcctca atccacttgc tcatcccgtc gcgctccaac atccactgcg gcttaccacg
     4381 tcagcgtggg ctgcacacat ccccttcgga atgtttctgg tggatatcgc acgccccgcc
     4441 ttgctggtcg aactcggtac ttatacgggg gtgtccttta gcgctttctg tcaggccgtc
     4501 gaggggttgg gattagagac gcgctgccac gccgtagata catggaaggg ggatgaccag
     4561 acaggctact acggcgggga gacccttgcc gatttaaagg ctcacatcga aacccactac
     4621 aaggggttcg ctcaattgat cgagagcact ttcgatcaag ccctccacag atttgaggac
     4681 ggcacgattg acctgctcca tattgacggc caccatgcct acgaatcggt gagacacgat
     4741 tttgagagtt ggctacccaa aatgagcgcg cgcggagtcg tgctcctgca tgatacgaac
     4801 gtccgcgagc cggacttcgg cgtctggaaa ttatgggaag aacttaaagt ccgctatccc
     4861 catttcgagt ttatacatga gcacggtctg ggagttcttg ccaccggaac tgagatgtct
     4921 gatggcctgc gcctgttgac cggaacctcg cccgaagagg ccgctgtctg gcgtaaattt
     4981 ttccatcaac tcggccaccg tttcagggtg cagttggata aagagaacga gattcacctc
     5041 ctcacggcgc agttggaggc tcacgccctt caacttcaaa ctgtctccca cgaactccgg
     5101 aacattctca actcaagaac gtggcgttcc ctcggtcgtt atatgtggct gaagaacttt
     5161 ctacagatgt ccgccgcgcg cctccggcaa aacttgcgga ctacccgaaa ggatgctcaa
     5221 taaaagtctc tctgcccgcg ataaaaaggc gggcacttaa catgttttcc agcggctgcc
     5281 ctctcccctc gaacataaat gtggaaggca acgttatgag tcggatgaag tcgagtaaga
     5341 gtcacgatgc cagttagtga gatgatgacg cccgaacgct ggcggcaggt cgaaaccgtt
     5401 ttccagacgg ctctcgacat cacgcgggaa cacagagagc gatttttggc ggaagcgtgc
     5461 gcgggcgacg aggttttacg ccgcgaagtc gagcatatga tcgagggtta cgaggcggcg
     5521 gacgaatttc tcgaactcga gcggcactac tcatttctga gagcaatcag cgggtctaca
     5581 cgcgtcgcgc gtcgcgcagg aatgtagcaa gccaggagtc ccacgacgga gagcaccaac
     5641 gccaccacgg caaacgtgac ggggtctgcc gcactaacac cgaagagtaa gctcttgagc
     5701 aggcgcgtta cgaggaaagc ccctaccata cccaccgcca cccctaagac cacgagccgc
     5761 atcccctgtc cgaccacgag gcggagcaca tccgctctct gcgcgccgag cgctaacctg
     5821 atgccgatct cgtgcgtccg ctgcgtgact gcgtaactga ggactccgta aatgcctacg
     5881 accgcgagca ggaaagccag tgtggcgaag cctgcgaaca ggagcacgat cagtcggcgg
     5941 ttggcgactg acgcggagcg cacgtcttcc atcgttctga tgtcataaac aggctgtgac
     6001 ttatcaatcg cgagtacggc ttcacgcacg gctcccgcga gcgactccgg ctccacgctc
     6061 gtgcgcacgg caagcgagag cgcgccccac gtcgcgttct gcgcctgtgg cacgtagacc
     6121 tgcggcctgt cttcgccctc catttgcccc atcttcacgt cggcgacaac acccacgatc
     6181 tcacgcgcga actcctcgtc gcgccatgcc gtcaacctct tgccgaccgg gtctatgtca
     6241 gggaatgctc ttcgtgcgag gctttcgttg atgatcgcaa ccggcggcgc gtctgccgcg
     6301 tccctgtccg taaacacgcg tccactcttg agtgggatgc gcatcgtttt tatataaccg
     6361 ggagtggtca cgttatagct tgcgtcgaac gcatgctcgg tcgtgagcgg attgccctca
     6421 cgtacgaaac cacgcgcgac cgaaagggtg gtgccgccga gcgggaggct gatcgcggca
     6481 cctgcggctt caactcccgg cagcgcctcg acgcgttccg tcaactgacg aaaaaagttc
     6541 acgcgctgct gcggatcgga gtaacgtgtc gaaggcaggc tgacgcgcat ggttaaaacc
     6601 ttctccgggt cgaagccggc atctacggcg tgaagtagaa ggaaacttct gatcagcagc
     6661 cccgccccga cgaggagcaa taaggaaagc gcgacctccg aaatgaccaa cgccgcacgg
     6721 acgcggttgc gccccaaccc tccggacgac ccgaaggagc cttccttcag aacttcgttc
     6781 agatcagttt tagacgcctg caacgccggc gcgattccga acaacagacc gaccacgcag
     6841 accgccccgg cagtgaagcc gagcacgcgc cagtccatgc tgacctcatt taatctcggt
     6901 agattcgccg ggctaattga taccagcagg tccatcagga agaaactcaa gaccagacca
     6961 accgcgcctc ccaacgttgc cagcatcaag ctctccgtca gcagttggcg gatgacgcgc
     7021 aggcgtccag ccccgagtgc cgtgcgcagc gcgatctccc tgcgtcgtcc cgccgcacgg
     7081 gcgagcagca gattagtgac gttggcacac gcgattaaaa gcacgaaccc gacggctccg
     7141 agcaagagcc acagagatgt gctcacgtct ctgacagtcc gttcgtgcaa cggggtgagg
     7201 cgcacagacc atccgccatt cgtctcagga tactgctgtc ccaggcgact attgatgatg
     7261 ccgagttgcg tctccgcttc ctccaacgtc acgtccggct tcaggcgagc cagcacgttc
     7321 aaatagcggt tgtcgcgctt ttgggctgac ttctccggtt tgagcggcgt ccacatctga
     7381 gaagcccttg ggaagtcaaa accggcgggc atgacgccaa caaccgtgac gggtgtgccg
     7441 ttcactaaga gacgggttcc cacgacgccc gggctggcgc cgaaccgccg ctgccagagg
     7501 gcgtggctga gtatggcgac atcttcccca ccaggagatt catcttccgg cagcaaggcg
     7561 cggcctagcg cagggctggc tccgagggtt gggaagaagc cggcggtgac cgacacgcgc
     7621 ggcacacgct caggttcccc tacctcatct gacaggtttg cgctgccggt aacgtaagcg
     7681 gacatgcttt caaaatcact agcctgcttc tcccaatccg agtagtccgc cggcgagaga
     7741 ttgctcgcgg ttatccccct cgcctggtga accccgtcga accagatgat cctttcggca
     7801 tccgggaacg gcagtggacg cagcagtacc gcattgacga cgctgaacag actcgtgttc
     7861 gcgccgatgc ctagtgccag cacgagtacg gccacggtgg tgaatccaaa gttcttccac
     7921 agcatacgcg tgccgtagcg acagtcctgc cacagggatt cgagccagcg accgcccctg
     7981 acctcataac cctgctcttt cacccgcgtg aatctgccga agcgccgctc ggcatctcgc
     8041 cgcgcctcct cgggagacat gccctgcctg acattttcct cctcccgcat atcgatgtgg
     8101 aagcgcatct cctcatcgat ttcgttgtgg atcgcctctt tgcagatcag tgcgcgcacg
     8161 cgcctatata aacccttcaa ccacatgcct tcatgactcc cttcaggccg cctgcatgac
     8221 cttgtcgata gctagcacca tcaaccgcca gtgctcctct tcgaccccta gttgcctgcg
     8281 ccccgtgcgc gtcaacgtgt aaaagcgcgc gcggcggttg ttgtcagact tgccccactc
     8341 ggacttgatc cagccccgct cctccagccg gtagagcgcc gggtagagcg acccctcctc
     8401 gaccttcagc atgtcctcag ccatctgctc gatctgctgg cctaccccgt agccgtgcaa
     8461 agcccctacg gagagcgcct tgagtatgag catgtcgagg gtgccctgca gtaggtctgc
     8521 tttttgtttt gccatcagtc cctcctagat gttctaggga agataatcaa ctttccctag
     8581 aacgtcaagg gaagatttgt gtgggttgtc gcttactcgg ccttggcagg agtggaggag
     8641 ttaaataacc tggtcgcagt gcgcgcgcct gcgcaccgtc ccgagcagtg ccctcacctc
     8701 ctcgtccgac agagcctcga cgctctcggt ctggtaaggt ttaggcgcac gcaggcgggc
     8761 aagcctcgcc ggatccctgt gtacatactg atccacttct gaatccttca aagcccacga
     8821 gtagaaggac gagagcaggc aggcgcgctg atagctcgtc cgcggcttta ctcctactcc
     8881 cttccagttc acgcagccag tcttgaacat cggctggttc gacctcgccg ggcagcttcc
     8941 cgtatgtgcc ggaagaagga gagcacaatg cgcggctcgt ccctcagtag gtcttgacgc
     9001 cgcccactct cgcccgtcgt gccagcagct cggaatgaat ccgcccccgc aacacgactt
     9061 gcaggtcgtc gtctcgtaac tggagggctg tactactgga aggagtgctc ataatgcttg
     9121 tcggctttat ctgatatcaa tgacttccgg ccattcgagg tcttcgatat cttgtgccgc
     9181 cgggtcaacc gttgacttca tcacaacccc ctccgtcccg catagctcat aacccatcgg
     9241 ccccagcgac ttctcttcac gacgcttctc gtccggctgt tcctcgctgc gcccgacgtg
     9301 ccattgaggc ccgggcgcgg gattcatcgc gtaccatgtc agcgcgaacg ccaacacaag
     9361 cgccaacaat tgcaatcgga ttctctgtat gacctgctct tcccacatga aaaactcttt
     9421 tcagctgctc ccttccctaa aatccgcttc tttataacat ggaaaccttg atgggtacac
     9481 atctagcatc tttgtgtatc caacagttca attcaattga atattgcttt ttttatatgt
     9541 aataattact tctgtgtaac gcaatcttga ttgggaggtt caatccagcc ttgagagcta
     9601 cccgtcgccg tcaacgtcgc cctgacccgc tcgcctcgcc tgacgctgtg cgtaatgtca
     9661 tcaatcaact gcgtgacgca ctgccagata ttatccctca gaaagacaaa gacctggtac
     9721 gaatgcttcg cgcagctcgg catgcccagc gatatccagc gaccgacaca aagcgtggcc
     9781 gccccggcag gtggaggcgc gaagacttgc ttaaaattgc cacacggctc ggcgacatac
     9841 ttgagcgtga aacatcatca cacatctcgg tcgcgagttt tgttgaccac tatttgcgtc
     9901 tactcgaatt ccctgctgat gtgagcgaag cactcgcttc cggacagatc aatctattcg
     9961 aagccgaaca actcgccaga attactccag agaggcttca ggtcactcca agccaagccc
    10021 gtcgcacgcg caccgacttg ctctcgacgc atctgcaaac taaagcctcc ggcactcagt
    10081 tacgccagcg tgtaaacgaa ttgctaaagc agcccgaggc tgcgactcag tctgatgaat
    10141 cgtttcttga gtacttggag gatttggaag atttcgaccc ttacgacccg actcatctgt
    10201 tttgggagca aatcaagcaa ctcggttttg ccttccgcga gatacgacgt gaagatgtgc
    10261 tggacgaaga gatcgaagag ttgttgaaag cgagtgaacc cattatggct gtattgagca
    10321 gaattcagcg tcgtaaggag cagaggggga tgacaaaatt gcagctctaa accagtctcg
    10381 ttcagtaaaa cagg
//
LOCUS       DASWNS010000586         7146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_100107, whole genome shotgun sequence.
ACCESSION   DASWNS010000586 DASWNS010000000
VERSION     DASWNS010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100107"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="VGB76_20175"
     CDS             <1..1558
                     /locus_tag="VGB76_20175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101252.1"
                     /translation="TLSAADAAVHIIDNPFTPQFFHHHQVGVTLFLIAALGAVFLKGF
                     KEAIGIAVVIVVVYILLNLIVLSVGFYQLATQPQLILSWKDALFARHSNPLLMIGAAL
                     VVFPKLALGLSGFETGVVVMPLVEGEAGDDRRKPMGRIKNTRKLLKAAALIMSVLLIA
                     SSIVTSMLIPADAFRPETATTPAGAASGRALAYLAHTFLGDIFGTAYDVSTILILWFA
                     GSSALAGLLNIVPRYLPRYGMAPDWAKATRPLVLVFTIICFAVTVLFKANVEAQGGAY
                     ATGVLALMSSAAIAVALSVWREARKWRWAFLLIALVFIYTTVVNIIEQPEGLKIASFF
                     IGFIIIVSLISRVSRTTELRVERVELDETARAFVEEANRRGEIRIVTNRCDRGDVSEY
                     EFKEREKRADNHIPTRDPVLFFEVKPGDASEFAGVLRVSGADVEGYHVLRAESPAVPN
                     AIAAFLLYLRDTTGKVPHVYFGWSEGNPLAYLFKYVAFGEGDTAPVTHEVLRQAEEDP
                     EKRPVVHVGG"
     regulatory      1616..1811
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1901..3871
                     /locus_tag="VGB76_20180"
     CDS             1901..3871
                     /locus_tag="VGB76_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101253.1"
                     /translation="MSIQIVVPERPSRGFKKWLLKGRVEEMEGPHEREGQHHTHPWWK
                     VMCLTGVDYFSTLGYQPGIAFLAAGALSPIATLILVSLTLFGALPIYGRVAAESPHGE
                     GSISMLENLLARWKGKLFVLVLLGFVATDFVITITLSAADATAHILENPYAQRFNVLD
                     HPVIITLVLVAVLGAVFLKGFKEAIGIAVVLVLAYLLLNTVVIGRGIFEVVTHPEYLP
                     RWRDSLYTAHANPLMMLLAALVVFPKLALGLSGFETGVAVMPLVKGDREDTEKNPSGR
                     IRDTRKLLRTAALIMSVMLITSSFVTTLVIPPEAFQPGTSTIPRGEANGRALAYLAHH
                     FFGDAFGTIYDVSTILILWFAGASAMAGLLNIVPRYLPRYGMAPDWARATRPLVLVFT
                     AVAFAVTIFFKADVDAQGGAYATGVLVLMTSAAFAVMLSARRRGAKRWTIAFLLISLV
                     FGYTTILNVFERPEGLKIASIFIGGIVLASLVSRVWRTTELRVVELVLDAKAREFIEE
                     AGRRGDVRIVANRRDRGDVEEYMSKEREKRAGNHIPTRDAVLFFEVKAGDASEFSESL
                     KVRGVELDGYRVLRTKSPAVPNAIAAFLLHVRDATGKVPHIYFGWSEGNPLAYLFKYV
                     AFGEGDTAPVTHEVLRQAEKDPKRRPVVHIGG"
     gene            complement(3998..4384)
                     /locus_tag="VGB76_20185"
     CDS             complement(3998..4384)
                     /locus_tag="VGB76_20185"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEY0101254.1"
                     /translation="MSQAQATTKANGEWSPAETEFQDRNIRCVDCNEDFIWTGGEQVF
                     FHDKGLKNEPKRCKPCKQAKNERLAAIAAAQASGVRQRIEVSVNCAQCSQQTTVPFYP
                     SQGRPVFCRSCFLASRGAGTPDAPRT"
     gene            4739..6040
                     /gene="purD"
                     /locus_tag="VGB76_20190"
     CDS             4739..6040
                     /gene="purD"
                     /locus_tag="VGB76_20190"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY0101255.1"
                     /translation="MKILVIGSGGREHALCHTLKRTSKQPLELYCAPGSDAISEIARC
                     VPLGATDVSALAAFAETEKIDLTIVGSEAPLAAGLADEFERRGLAVAGPRAEAAQLES
                     SKAFAKEFMARHKIPTARFRVAASAREAREILASGEFGGEDSPVVVKADGLAAGKGVV
                     VAATRREALEAIAELMEGGRVGSEAARRVVIEEALGGAEASLLFWTDGETYALMPPAR
                     DHKRIGEGDTGANTGGMGAITSPDVLDKATEARVVLEIVEPTLAGLRAAGLEFRGVIF
                     IGLMLTPAGARVLEYNVRFGDPEAQAILPRLESDLGEIFAAVAHKRLKGVAVSWSEDA
                     SACVVLAARGYPERPETGARIEGLAGTGEAQLTPSVQIFHAGTRRADDGAWLTAGGRV
                     LGLTACAPTLDLALERCYEAARRIRWEGMQYRRDIGKFSKQ"
     gene            6321..7058
                     /locus_tag="VGB76_20195"
     CDS             6321..7058
                     /locus_tag="VGB76_20195"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEY0101256.1"
                     /translation="MSSWSRFKRSLRALFGGLIEKTEDPEMILNQTIREMRDRVPELN
                     NSVAQVMATERLLAKSKERLEGQVVDLDSKIKASIKLGRDDIATAYIGQLQQAQLDLN
                     KTGQQLQHAEMASKQALKARDNYMLQMQQRTAEAMQLINQSKQAKLQEQLAQTMETFQ
                     LGDDASTFGEMRDKIDRRSAAAEAKMQLGAASVDSQMQDIEREALDMQLQDKLLEYKQ
                     GMGLLPGSTSKQQGALPAAGEDRGRAS"
BASE COUNT         1372 a         2203 c         2197 g         1374 t
ORIGIN      
        1 cacgctctcc gccgccgacg ccgccgtcca catcatcgac aaccctttca cgccgcaatt
       61 ttttcaccac catcaggtcg gcgtcacgct ctttttgatc gccgcgctcg gcgcagtctt
      121 cctcaagggg tttaaggagg ccatcggcat cgccgtcgtg atcgtcgtcg tctacatcct
      181 gctcaacctg atcgtgctgt ccgtcgggtt ttatcaactc gcgacgcagc cgcagttgat
      241 cttgagttgg aaagacgctc tgttcgcacg ccatagcaac cctctgctga tgatcggtgc
      301 ggcgctcgtc gtcttcccga aactcgcact cggactctcc ggcttcgaga cgggcgtggt
      361 cgtcatgccg ctcgttgagg gcgaagccgg cgacgaccgg cgcaaaccga tggggcgcat
      421 caagaacacg cggaaactgt tgaaggccgc cgcgctgatc atgagcgtgc tgctcatcgc
      481 cagcagcatc gtgacctcca tgctgattcc cgcagatgcg tttcgcccgg agacggcgac
      541 tacgcccgcc ggtgcagcga gcgggcgcgc gctggcgtat ctggcgcata cgttcctcgg
      601 agacatcttc ggcacggcct acgacgtgag cacgatcctg atcctgtggt tcgccggctc
      661 gtccgcgctg gccgggttgc tcaacatcgt gccgcgctac ctgccgcgtt acggcatggc
      721 tcctgattgg gcgaaggcga cgcgcccgct cgtcctcgtc ttcaccatca tctgcttcgc
      781 cgtgacggtg ctcttcaagg caaacgtcga ggcgcagggc ggggcttatg cgacgggcgt
      841 gctggccttg atgagttcgg cggccattgc ggtcgcgctc tccgtctggc gcgaggcgcg
      901 caagtggcgt tgggcgtttc tgctgatcgc gctcgtcttc atctacacca cggtcgtcaa
      961 catcatcgag cagccggaag gactcaagat cgcctcgttc ttcatcggct tcattatcat
     1021 cgtctcgctc atctcgcgcg tctcgcgcac gaccgagttg cgcgtggaac gcgtggaact
     1081 cgacgagact gcgcgcgcct tcgtcgaaga agcgaaccgg cgcggcgaga tacgcatcgt
     1141 gacgaaccgc tgcgaccgtg gcgacgtgag cgagtacgag ttcaaggaac gggagaagcg
     1201 cgccgacaat cacatcccga cgcgcgaccc cgtgctcttc ttcgaggtca aacccggcga
     1261 cgcttccgag tttgcgggcg tgctcagggt gtcgggcgcg gacgttgaag gctatcacgt
     1321 gctgcgcgcg gaaagtcccg ccgtgccaaa cgccattgcg gcctttctac tctacctgcg
     1381 cgatacgacg ggcaaagtgc cgcacgtcta cttcggttgg agcgaaggaa atccgctggc
     1441 ctatctcttc aagtacgtgg cgtttggcga gggcgatacc gcacccgtca cgcacgaagt
     1501 tttgcggcag gcggaagaag acccggagaa gcgtccggtg gttcatgtcg gcggttaagg
     1561 gaaagttatt gacggttgct ttacgacgag cagaggcgta acatccgcag cgttgaaaat
     1621 ttgtcagccg aagttgttca cggcgtcgca ggacgttaga gggacaacac gggggaacca
     1681 acgtttcgct cttcaggcga gacacggggc gcatcgtgag cgttcgactc caacagggtc
     1741 ggactcttcg cgaaggatac ttccaaatcc cagcccgtca gctaacctcg caggctttta
     1801 gggaagggca actccgcgca agcagtatct ctaaaggcgc agggcttcat gctctgcgcc
     1861 ttttttcgtc ttgcgcggca ctagcaacga aggagcttct atgtccattc aaatcgtcgt
     1921 gccggaacgc ccgtcgagag gttttaagaa atggttactt aaaggccgag tcgaagagat
     1981 ggaaggaccg cacgagcggg aagggcagca tcacacgcac ccgtggtgga aggtgatgtg
     2041 cctgacgggc gtggactact tctccaccct cggctatcaa ccgggcatcg cctttctcgc
     2101 cgccggtgcc ctctctccca tcgccacgct gattctcgtc tccctgacgc tcttcggcgc
     2161 gcttcccatc tacgggcggg tggccgccga aagcccgcac ggcgaaggct ccatctcgat
     2221 gctcgaaaac ttgctggcgc gctggaaggg caaactcttc gtgctggtcc tgctcggttt
     2281 cgtcgccacc gacttcgtga tcacgatcac gctctccgcc gcagacgcca ccgcccacat
     2341 cctcgaaaac ccctacgcgc aacggttcaa cgtactcgat catccggtca tcatcacact
     2401 cgtgctggtc gcggtgctcg gcgcggtctt cctcaaaggg ttcaaggagg ccatcggcat
     2461 cgccgtcgtc ctcgttctgg cctacctgct gctgaacacg gtcgtcatcg gtcgcggcat
     2521 ctttgaagtc gtgacgcacc cggaatacct gccgcgctgg cgcgactcgc tctacacggc
     2581 gcacgcgaac cctctgatga tgcttctggc cgcgctcgtc gtcttcccga aactcgcgct
     2641 cggactctcc ggcttcgaga cgggcgtggc cgtcatgccg ctcgttaaag gcgaccggga
     2701 ggacacggag aagaacccct cggggcgcat ccgcgacacg cgcaaactcc tgcgcacggc
     2761 tgccctcatc atgagcgtca tgctcatcac gagtagtttt gtgaccacgc tcgtcatccc
     2821 gccggaagcc ttccagccgg ggacttcgac gatcccgcgc ggcgaagcca acgggcgcgc
     2881 gctggcctac ctcgcgcacc atttcttcgg cgacgccttc ggcaccatct acgacgtgag
     2941 caccatcctg atcctctggt tcgcgggcgc gtccgcgatg gcggggctgc tgaacatcgt
     3001 gccgcgctac ttgccgcgct acggcatggc tcccgattgg gcgcgcgcca cgcgcccgct
     3061 cgtcctcgtc ttcactgccg tcgccttcgc cgtcacgatc ttcttcaagg cggacgtaga
     3121 cgcgcagggc ggggcttacg cgacgggcgt gctggtcttg atgacctcgg cggccttcgc
     3181 cgtcatgctc tccgcgcggc gcaggggggc aaagagatgg actatcgcct ttctgttgat
     3241 ctcgctcgtc ttcggctaca ccaccatcct caacgtcttc gaacgaccgg aaggcttgaa
     3301 gatcgcttcg atcttcatcg gcggcatcgt gctcgcgtcg ctcgtctcgc gcgtgtggcg
     3361 cacgaccgaa ctacgcgtcg tagagttggt gctggacgcg aaggcgcgtg agtttataga
     3421 ggaagcgggc aggcgcggcg acgtgcgcat cgtcgctaac cggcgcgacc gtggcgacgt
     3481 ggaggagtac atgtcgaagg agcgggagaa gcgcgcaggc aatcacatcc cgacgcgtga
     3541 tgccgtgctc ttcttcgagg tcaaggcagg cgacgcttcc gagttttccg agagcttgaa
     3601 agtgcgcggc gtggagttgg acggctaccg cgtgctgcga acgaaaagcc ccgctgtacc
     3661 gaatgccatc gcggccttcc tcctgcacgt ccgcgacgcg acgggcaaag tgccgcacat
     3721 ctatttcggt tggagcgaag gaaatccgct ggcctatctc ttcaagtacg tggcatttgg
     3781 cgagggcgat accgcacccg tcacgcacga agttttgcgg caggcggaga aagacccgaa
     3841 gcgtcgtccg gtggttcaca tcggcgggtg agagagactg tcgggagcag ctttgcgtcg
     3901 cccgcacaca ctgcggcgag gccgcacgtt tccgccgcag tgagaattgt cgattagcga
     3961 tctgcctgtc gggaagtaag gcttatagcg gcgacgttta agttcggggc gcgtccggcg
     4021 tgcccgcgcc gcggcttgcg aggaagcaag agcggcagaa gaccggacga ccctgcgagg
     4081 gataaaaggg aacggtcgtc tgttgactgc actgtgcgca gttgacggag acttcgatgc
     4141 gttggcgcac gcccgaagcc tgcgcggcgg caatggcggc cagccgttca ttcttcgcct
     4201 gcttgcacgg cttgcagcgt ttcggttcgt tctttagacc cttatcgtga aagaaaacct
     4261 gctcgccgcc cgtccagatg aaatcttcgt tacaatcaac gcagcgaata ttgcggtcct
     4321 gaaattcggt ttcggcggga ctccactccc catttgcttt ggttgttgct tgagcttgtg
     4381 acatgtctgt aaccccccac ggatgcactt cccggagtcg aactttcggt gagagaagtt
     4441 ttttctcgaa ggaaatctgt tttttgcaga tcggagtctc gcgtaaagac ccctccctta
     4501 atctatgcgt tttaaagatc acgcgagcca cgcgcgcctg taaagccagt cgcagccgcc
     4561 catcgttgac cgagttacta tgcaaagtat aactatcaca aaactgtgtc aaggctttcc
     4621 gagcaggctc gaaaggtcgc actccgggca agtcgtcttg acaccacttt cgcgccgtgc
     4681 cagcatccgc gcgttcaaag ctcaaattac ttctcgtgag cgttaaaaat ttctctctat
     4741 gaagattctg gtcatcggct caggcgggcg cgaacacgcg ctctgccata cgctcaagcg
     4801 aacttccaaa caaccgctcg aactctactg cgcgcccggc agcgacgcca tctccgagat
     4861 cgcgcgctgc gttccgttgg gggcgacgga cgtgtccgcg ctcgccgcgt ttgccgaaac
     4921 ggaaaagatc gatctcacga ttgtcggcag tgaagccccg ctcgccgccg gactcgcaga
     4981 cgaattcgag cggcgcggcc tcgccgtcgc gggaccacgc gccgaagccg cacaattgga
     5041 gtcgagcaag gcgttcgcca aagaatttat ggcgcggcac aaaattccga cggcgcgttt
     5101 tcgcgtcgcg gcgagtgcgc gggaagcaag ggagatttta gcgagcgggg agtttggcgg
     5161 ggaagattcg cccgtcgtcg tcaaagccga cggactcgcg gcgggcaaag gcgtcgtcgt
     5221 ggccgcgacg cgacgcgagg ctttagaggc gatcgcggaa ttgatggaag gcgggcgcgt
     5281 ggggagcgaa gcggcacgtc gcgtcgtcat cgaagaagca ctgggcggag ccgaagcctc
     5341 tctcctgttc tggactgacg gcgagactta cgccctgatg ccccccgcgc gcgatcacaa
     5401 acgcatcggc gaaggcgaca ccggggcgaa tacgggcggc atgggcgcga tcacttcacc
     5461 cgacgtgctc gacaaagcga ccgaggcgcg cgtcgtcctt gagatcgtcg agccgacgct
     5521 ggcgggcttg cgcgccgctg gactcgaatt tcgcggcgtg atcttcattg gcctgatgct
     5581 gacgcctgcg ggggcgcgcg tgcttgagta caacgtgcgt ttcggcgacc cggaagcgca
     5641 ggccatcctc ccgcgactcg aaagcgacct cggcgaaatt ttcgcagccg tcgcacacaa
     5701 aagactcaag ggcgtagcgg tcagttggtc tgaagacgcg tcggcctgcg tcgtcctcgc
     5761 cgcgcgcggc tatcccgagc gacccgaaac cggcgcacgc atagaaggtt tggcaggcac
     5821 gggcgaagcg caattaacgc cctcggtaca gattttccac gctggaacga ggcgtgccga
     5881 cgacggtgcg tggctgactg cgggcgggcg cgtgcttggc ctcaccgcct gcgcccccac
     5941 cctcgacctc gcgctcgaac gctgctacga agccgcccgg cgcattcgtt gggaagggat
     6001 gcagtatcgc cgcgacatcg gaaaatttag taaacagtga gcggcgagca gtaagcagtt
     6061 aagaggtcga ggggcaaccg tctgctttct cttaactgct tatcgtgcgc cgctcactgc
     6121 acactttatg gcggctctag tctgccaacg gcgactcctg ctatactcgc tgcatgagcg
     6181 gatcgtcgct gaaaatgaac cgcaggagtt gcgtttacgt actaacagag gcgcgtggca
     6241 gattcgggcg cggaaagttt ggcagcagcg caacggtcga agccggtgcg ctgaatagtt
     6301 agttcagagg agcagaaaag atgtcatcgt ggagccggtt taagcgttct ttgcgcgcat
     6361 tgtttggcgg tctgatcgag aagaccgaag accccgaaat gatccttaat cagaccattc
     6421 gcgagatgcg cgaccgcgtg cccgaactca acaactcggt cgcgcaggtg atggcgaccg
     6481 aacgcctgct cgccaagagc aaagaacgcc tcgaagggca ggttgtcgat ctcgattcca
     6541 agatcaaagc ctccatcaaa ctggggcgcg acgacatcgc caccgcttac atcggccaac
     6601 tgcaacaagc ccaactcgat ctcaacaaaa ccgggcagca acttcagcac gccgaaatgg
     6661 catccaagca agcgctcaag gcgcgcgaca actacatgct tcagatgcag caacgcaccg
     6721 ccgaagccat gcagttgatc aaccagtcga agcaggccaa gttgcaggag caactggcgc
     6781 agacgatgga gacgttccaa ctcggcgacg atgcttcgac cttcggcgag atgcgcgaca
     6841 agatcgaccg tcgctccgcc gccgccgaag ccaagatgca actcggcgcg gcctccgtcg
     6901 attcgcagat gcaggacatc gaacgcgagg cgctggacat gcagttgcag gacaagttgc
     6961 tggagtacaa acagggcatg ggactgttgc cgggttcgac gagcaaacag cagggcgcgc
     7021 ttccggcggc gggcgaagat cgaggacgcg cttcttagcg ttcgacacgg ggaagcgggc
     7081 gcgaagttcg gttgaaagca gcaagcgggc aaacggtcaa cggtaaactt ttacggcagt
     7141 tcggtt
//
LOCUS       DASWNS010000587         5181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_176999, whole genome shotgun sequence.
ACCESSION   DASWNS010000587 DASWNS010000000
VERSION     DASWNS010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176999"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(672..878)
                     /locus_tag="VGB76_20200"
     CDS             complement(672..878)
                     /locus_tag="VGB76_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101257.1"
                     /translation="MSVEYGKAVSFDFPAGAFPAGARQRLRAVVIVAPDPSGIAPCVM
                     PSVEVLNNETGKTVMLYPGSLIGW"
     gene            1400..1630
                     /locus_tag="VGB76_20205"
     CDS             1400..1630
                     /locus_tag="VGB76_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101258.1"
                     /translation="MNALRSARLMALKPGMKIRDDELTRETVGIKSLTPAPGDTEDLK
                     IYNDEVAKYMIKWTQLGWSEQIQWSGMKAACR"
     gene            1997..2530
                     /locus_tag="VGB76_20210"
     CDS             1997..2530
                     /locus_tag="VGB76_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101259.1"
                     /translation="MNDQSSSPAPNVNPTDGRQSNALNKENWIVESLPYTEDPPAKGF
                     LQLCADRRFHKCIQRAFKEDAGLDSREDYWIHADAGGTPKMECQRVTPDYCYINKEVR
                     LMGWSAHGDKCGGFGEEVPDDVIKRELCATMQRNVRKYALATHFIYFATVRDEETVLY
                     RMRAEPGEDIVCTQGSD"
     gene            2724..3995
                     /locus_tag="VGB76_20215"
     CDS             2724..3995
                     /locus_tag="VGB76_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101260.1"
                     /translation="MTVIYAQSKDRKTLADKLVAYSDLPRQEAENFASSAGPGPYVPL
                     PLMDSSAILKNLPEETRRTFRHRSSMLEDAITWTYPLAIDEELLEGGLVTTWLSEAAI
                     EYPRGTIAIQRKTNLGELIFLNQEFTFDIDSRKLVDNRPDFSPEALAGAQAEEFAFSW
                     ATLGLDIAKSLLGALAGKIGSAIFARLFPPSVPSYFEQVYQEFRKIVRQEIEENTIRL
                     LVGDVAAVQTHMSAYAQHRQRGNLDAAGQALLEAWNRSVQVTSRLREFPVAGLGPFLT
                     AGGLHLAIIQERALRDTTVNDPNDSSWADIYVDTAGKFSSYVGSQPDKIVSERAAQIT
                     PLTFTERWAHTPWGPIDGSFWFYADPGWVPPLRRQMFRSDGWKLQLAKDQRINHHLKV
                     TVPLTKALEPAKVTAHHWRVARETPLPQVET"
     gene            4176..4667
                     /locus_tag="VGB76_20220"
                     /pseudo
     CDS             4176..4667
                     /locus_tag="VGB76_20220"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         1153 a         1410 c         1552 g         1066 t
ORIGIN      
        1 ccagacaaca gtgatatcgc tcggagtttt atttccgctt gggttgatct gggcgaatgc
       61 attattgaat tcgagaaaga agcctccctt tgccaaaggt ggtaacccct gaagggggag
      121 agtacaactt acaagtaggg aggcagcgac tgtgaagacg acgagaggaa ttgccaatct
      181 gagttttgac cgtgacatat attgacctca ttattttacg aagtgaattc tgaaccttgt
      241 gagggaatag aactgaaacc ctcttcaggt cctgaagaat gcgattgata caatttttgc
      301 ttctatgtcg ttagcgtgcg catttgcgtc ctccttaatt caagtcttgg attgcctctc
      361 atttttcaac ccctgcgaag ttagtgcggg cgcgattctc acagacgaat agtttgtggg
      421 aagaggacga cgagggttga tgccttgccg cccgtaacat ttatcgagat caatcgtcat
      481 cgtcgccctc gaagggcttc ccggctttgt cgttcacgta aacgtcgaag acggcgaaca
      541 gcgggctggg atcggccaag ccgttcaggc tggcgtggtt ggtcaagagc agcgcgctgg
      601 cacgccgagg gcggcggctg tggtcgccgc ccccgcgtgc accggatgcg ccccggcggg
      661 cgggagcgct cctaccagcc gatgagggaa ccggggtaca gcatgacggt cttgcccgtc
      721 tcgttattca agacttcgac ggaaggcatg acgcagggcg cgatgccgct cgggtcgggc
      781 gcgacgatga cgactgcgcg caggcgttgg cgcgcccccg cgggaaacgc cccggcgggg
      841 aaatcaaagg agacggcttt gccgtattca accgacaccg cgcgatccgg aggaggtggc
      901 gagcggtgca gtgcgtccgc tccttccata cggccgagcg gacgctggaa ggggttgaat
      961 cgatgcatat gctgaggagg gggcaggtta agaggttgga cggaagggac gcggtaggtc
     1021 aggcgaagtt cgtcgagagc ctcttcggtg tcgccgcatg acggaattca ctaagcgatc
     1081 tcttctcggc tcaaagtaat tcccgcaaca caaccgtcta gagtgggtcc actctatgcc
     1141 tgatcgagcg cggcgtcggt gtgttagcac acagataacg gagacgtatc ggcgtatagt
     1201 tattctaact tctatactcc ttcccaactg ccagattgcg gagtctagat aagcgaggcc
     1261 gccacaagca tttccccggc ggcctcgttt tcctttcctc ctccgacttt ttgggtagga
     1321 aggactgttt cggctggttt tctaactctc aggatttcat cgacgggttt aatgcgcatc
     1381 ccagggccgg tactaacatt tgaatgcctt gcgctcggcg cggctgatgg ctcttaaacc
     1441 gggcatgaag atacgcgacg acgaattaac gcgcgaaact gttggaatta aatctttaac
     1501 tcccgcgccc ggagatacag aagatctcaa aatctacaac gatgaagttg ctaaatatat
     1561 gatcaagtgg acccaactgg ggtggtcgga gcagattcaa tggtctggaa tgaaagcggc
     1621 ttgccggtga gcatcctttc cagggcgttg agcagggact agccccgctt acgtgcagtc
     1681 gaaaggtagc ttccgcactt gatgcgtccg ccctcgcgct ggcgacgcga gaacggcgag
     1741 cgctgagccg cacagacgga gccaagcctg tgagaggagg ggatgaagtg gccgacaccc
     1801 cggcgtccct cttcattgac atatcctccc tgctggcata tcgttcgcgc actcaaaggt
     1861 tgcccttcct tctcgatccc acgaccccgg gcagtgtctc cctatagcta cgcaacctaa
     1921 cccgaaccac tcggcacagt agtggtttca gtcggaggcg gcactctcta acaaattccg
     1981 gcgataagga gatgtcatga acgaccagtc gagcagtcca gctccaaacg taaatcccac
     2041 cgacgggcga caatcgaacg cgctcaataa agaaaattgg atagtcgaaa gcctccccta
     2101 tacggaagac ccccctgcaa agggttttct tcagttatgc gctgacaggc gattccacaa
     2161 atgtatccag cgggcgttta aagaggacgc cgggctggat tcgcgtgaag actattggat
     2221 acacgctgac gcgggaggga cgccaaagat ggagtgccag agagtgacgc cggactattg
     2281 ttacattaat aaggaggtgc ggttgatggg gtggtcagcc cacggagata agtgcggggg
     2341 attcggggag gaggtgccgg atgatgtcat caagcgggaa ttgtgtgcaa cgatgcaaag
     2401 gaacgtaaga aagtacgcgc tggcaacaca cttcatatat ttcgcgacgg tgcgcgacga
     2461 ggagacggtg ttgtatcgca tgagagctga accgggtgaa gacatagttt gcacacaggg
     2521 ttcagattag ttcacgtgga taggtaagga ctcgaaagcc cgggttcctt tgacgacggt
     2581 ataacggcgg agtatggcgg gcgcgtcctg agacattgtg agtggccgaa gcgcccgccg
     2641 tgccgcgctc tacctcaact gaacttgggc ccggaaccag tatcagattg tgagataggg
     2701 cttttaaaga ggagagtgtc accatgaccg taatctatgc tcaatccaag gatcgaaaaa
     2761 cattggcgga caaattagtc gcctactccg accttccacg acaggaagcc gagaactttg
     2821 catccagcgc ggggccggga ccttacgtcc cgcttcctct gatggattct tcggccattc
     2881 tcaagaattt gccggaggag acgcggcgaa cattcagaca ccgatcctcg atgctggagg
     2941 acgcaatcac gtggacctat ccgcttgcca tcgatgagga gcttttggaa ggcggacttg
     3001 ttacgacatg gctgtctgag gccgctatcg agtacccgag aggaaccatc gcgattcagc
     3061 ggaagacaaa cttgggtgaa ctcatctttc tcaatcaaga gttcaccttc gacatcgaca
     3121 gcaggaagct tgttgacaac agacccgact tttctccgga ggcgctcgca ggagcacaag
     3181 ccgaggagtt tgcattcagt tgggctacat tggggctgga tattgcgaag agtctcctgg
     3241 gcgcgcttgc cggtaaaatc ggctcggcca tcttcgcgcg gctctttcca ccttcagtgc
     3301 cctcctattt cgagcaggtc tatcaggaat tccgtaagat cgttaggcaa gaaatcgagg
     3361 agaatacaat caggcttctc gtgggcgatg tggccgccgt ccagacccac atgtccgcgt
     3421 atgcgcaaca caggcagagg gggaatcttg acgccgcggg acaagccctc ctagaggcat
     3481 ggaatcgttc ggtacaagtg acgagcagac tccgagaatt ccctgtggca ggtctggggc
     3541 cattcctgac ggcaggcggt ctgcatctcg ctattatcca ggagcgcgca ttgcgcgata
     3601 cgacagttaa tgatcccaac gactccagtt gggccgacat ctacgtcgac acggcaggca
     3661 agttcagctc atacgtgggg tcgcagcccg acaaaatcgt cagcgagcgg gcagcgcaga
     3721 taacaccttt gacgtttacg gagcggtggg cgcacacgcc ctggggcccc attgacgggt
     3781 cgttctggtt ttacgcggat ccggggtggg tgccgccgct acgccgccag atgttccgga
     3841 gcgacggctg gaaattacag ttggctaagg atcaacgtat aaaccaccat ctgaaggtga
     3901 ccgttccctt gaccaaagca ctggaacccg ccaaggtgac tgcccatcat tggcgagtgg
     3961 caagggaaac tcccctcccc caggtcgaga cttagaatcg tgacacagcc atagaacaga
     4021 ttccgaggtt gaatgctcac gtccactgtg gagcccaacc gcgactcagt tccccgaaac
     4081 aaatgagggt ctgaggaaag tagagaagct aatccacagt tctgcctcct gaatagctac
     4141 gcggccgacg cacaaagcct tcaggcccct accgcaacca cgccatcgtc tacgcgctca
     4201 tcgataccgg catgcgccgg gcggcggtca ccaacctcga cctcgccggc gtggactgga
     4261 aggggcggac gctgagcgtc gtggagaagg gcggctacac gcacggctac tcgatcagca
     4321 aagagggctt acaggcgatc cctgattacg tcgagaacga gcggacgccg gacgccgagc
     4381 actggcggtc gccggcactc ttcccgacag cctgtaacaa cgcgcgctca ggcgagcgcc
     4441 tcagcgtccg ctccgtcaac gtgatctagg acgaggtctg tggcatggcc gaggtgtagg
     4501 gtaagacgcc ccactctacg cggcacgcga tggggaagca cattatcgag aagaccggcc
     4561 acatcgccgc ggtgcagcgc cagctcgggc acaagaacgc ggtctactct atgcagtatg
     4621 cgaggatcac cggcgaggag ctgcgcgacg tgatagacga caggtaaagg gcggccggct
     4681 tgaattacct cgtcgaggtg tcaggccggc ccgacggggc ggttagttgt cggcggctga
     4741 cgtcaagata ctttttgaaa atttttcgcg cgggctcgcg ggtgagcgtg attaagttca
     4801 ccgggttgcg tcgccagtcg cgggtgataa gtgtcgcgcg ctccgcgtct caggtaatcg
     4861 cccggccggt atcctgcaaa tcacggatgc tccatcgggt cggctcaccg cggctgagga
     4921 gccgcccggc tgtgtccacc cagtattcgg cgttgccgta gatgcccctg cctccacgct
     4981 gtagaggccg cacccgtccg ggttctgagt gctcgcgacg aactccctct tgcccgcgag
     5041 cacatagatc ctgagttcct tcagtccgtt cggcctgatc atttcgaggg catttcgaga
     5101 ccgggcggat gtagaagctg gcgacccgtt gaatagttct ttcactctca attccttcgg
     5161 tgtttgagct ttggtttcag a
//
LOCUS       DASWNS010000588         3760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_462848, whole genome shotgun sequence.
ACCESSION   DASWNS010000588 DASWNS010000000
VERSION     DASWNS010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462848"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /locus_tag="VGB76_20225"
     CDS             complement(<1..1938)
                     /locus_tag="VGB76_20225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0101261.1"
                     /translation="MSVPSTRPTETFTLLVVDDDEQTRASLQGVFERAQHQVKVAADA
                     PTALRMLHREPCDLILLDIELPGVDGLALCRLLRAQEATRRLPIIIISAHDDEERKVG
                     AFAAGADDFIVKPATQRELLLRVSTQIEAAQRERALVGSNRELGFLADLGRGLLVALE
                     PMQVVRRVAGATYEAANASLCAAMLETPQRIDESNSSAPDREAVAETKLARGAVCVFD
                     RDGSAEGDALIDNAKLSVWLDSNPQAAVRLEESAKFFLRDAAHRVEYVAPLRFGGRAL
                     GALVAAYDRPEDCGETESRLIDAAAQQAALAAHISSLYEAARSSSESLAKEVERRTAE
                     AEAQRRFTEAIIDTLPVSLYAVNRDYRIVAWNRNRELGGQGIPRGQALGRNIFDVLTR
                     QPRENLEREFKRAFKTGRIERLEQESIAADGTAKHWLVSKVPMRVEGDEVSHVITVGE
                     DITTRVEANRAVARTEKLAAVGRLAAGVVHEINNPLATIAACAEALETRVAEGAFGKS
                     AEVDDLREYLSLIRSEAFRCKTITNGLLDFSRNRAGQHTPVELGEVLEATARLLRHQK
                     RSRNVEIKVEAQGDVAPVSGDAGQLQQAVIILAENAIDAMPHGGTLLLRAQTEGAGVR
                     VEVCDTGVGIPQENLARIFDPF"
     gene            complement(2259..>3760)
                     /locus_tag="VGB76_20230"
     CDS             complement(2259..>3760)
                     /locus_tag="VGB76_20230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0101262.1"
                     /translation="ERIDYPYPIVPIVRHHHERWDGLGYPDGLRGEEIPWTARIFAVV
                     DCFDSVREDRPYRRGMTVEEASAVLLRGAGTHFDPKVVALFLKHLPEFEAEVVERGLD
                     QHGFTSREAEPRALVEASGAAATQPPSINQNCYTVPAYLEQIKSAHREVYALYEIAST
                     FGASLEIADRSQILVNKVGHIVPFDTCAVYLFDEEKNYATVAHVSGLNAELLRGHCVA
                     PGEGVIGFVIANRRPATGFDPMLDFAAFKLPEEENYGSMIALPLIKDERLLGVLAVYA
                     LKSGQYTDDYLHLLDTVARLASDAFSNAMQHAEAESNALTDALTSLPNARALHLRFAE
                     EEARARRNGTSFQVVMLDLDDFKIVNDTYGHSTGDQMLREVARVLNGQLREYDFLARY
                     AGDEFVALVQDLSHAQVDDLRGRIEAAIAGLSLRVPPDKRARVGISVGSARFGAHGET
                     LQQLLIAADQAMYSVKSDRKQRRRHTGTGGTPLSLVETPTGELASTAIN"
BASE COUNT          635 a         1191 c         1227 g          707 t
ORIGIN      
        1 gaacggatcg aagatgcgcg ccagattctc ctgcggaatc ccgacgcccg tatcgcagac
       61 ctccacgcgc acgcccgcgc cctccgtctg cgcgcgcagc aggagcgtcc cgccatgcgg
      121 catcgcgtca atcgcgttct ccgccaggat gatgacggct tgctgcaact gccccgcatc
      181 gcccgacacc ggagcgacat cgccctgagc ctcgaccttg atctcgacgt tgcggctgcg
      241 cttttgatga cgcaggagac gcgccgtggc ctccaacact tcgcccaact cgaccggcgt
      301 gtgctgcccc gcgcggttgc gactgaaatc gagcaggccg ttcgtgatcg tcttacaacg
      361 aaaagcctcg ctgcgaatga gcgacagata ttcgcgcagg tcgtcaacct ccgcagactt
      421 cccgaacgcg ccttcggcca cgcgcgtctc cagagcttcg gcgcacgcgg cgatggtggc
      481 gagcgggttg ttgatctcat gcacgacgcc tgcggcgagg cgaccgacgg cggccagttt
      541 ttccgtgcgt gccacggcgc ggttcgcctc cacgcgcgtc gtgatgtctt cgcccaccgt
      601 gatgacgtgc gaaacttcgt cgccctcgac gcgcatcgga actttgctga cgagccagtg
      661 tttcgccgtg ccgtccgccg cgattgattc ctgttccaga cgttcgatgc gcccggtttt
      721 gaaggcgcgt ttgaattcgc gttccaaatt ttcgcgcggc tgcctggtca gcacgtcgaa
      781 gatatttcgg cctaaagcct gcccgcgcgg gatgccctgc ccgccgagtt cgcggttgcg
      841 gttccacgcg acgatgcggt aatcgcgatt cacggcgtag agcgagacgg gcagcgtgtc
      901 gatgatcgct tccgtgaaac gcctctgcgc ttcggcctcg gctgttcgtc gctcaacctc
      961 tttcgccaga ctctccgaag aagagcgcgc cgcttcgtag agcgaagaga tatgcgcggc
     1021 gagcgcggcc tgttgcgccg ccgcatctat gagacggctt tcagtttcgc cgcagtcttc
     1081 cgggcggtcg taggcggcga cgagtgcgcc gagcgcacgc ccgccgaacc tgagcggcgc
     1141 gacgtattcg acgcggtgcg ccgcatcgcg cagaaaaaac ttcgcgctct cttccagacg
     1201 caccgccgcc tgtggattcg agtccagcca gacgctcaac ttcgcattgt ctatcagcgc
     1261 gtcgccctcc gcgctgccgt cccggtcaaa gacgcagacc gcgccgcgcg cgagcttcgt
     1321 ctccgccaca gcctcgcggt ctggtgcgct gctgttgctc tcgtcaatcc tctgcggcgt
     1381 ctccagcatg gccgcgcaga gcgaagcgtt ggccgcttcg taggttgcgc ccgccacgcg
     1441 cctgacgacc tgcatgggtt cgagcgcgac gagcaggcca cgcccgaggt ctgccagaaa
     1501 tcccaactcg cgattgctgc cgacgagcgc gcgttcgcgc tgcgccgctt cgatctgcgt
     1561 gctgacgcgc aacaacagtt cgcgttgcgt cgcgggtttg acgatgaagt cgtccgcccc
     1621 tgcggcaaac gcccccacct ttctctcttc gtcgtcgtgc gcggagatga tgatgatcgg
     1681 cagtcgtcta gtcgcctcct gtgcgcgcag gaggcggcag agggcgaggc cgtccacgcc
     1741 cggcaattcg atgtcgagca ggatcagatc acacggctcg cggtgcagca tgcgcaaggc
     1801 ggtcggcgcg tcagccgcga ctttgacctg atgctgtgcg cgctcgaaca cgccttgcag
     1861 ggaagcgcgc gtctgttcgt cgtcatcgac gacgagcagg gtaaaggttt cggttggtcg
     1921 agtggaagga accgacatgc atgctttcgt tacggacgcg ccgcgccgga gacggcggct
     1981 gttggcgcag acaatcgaac agttaaagag gagcagtcac ggctgcgggc gggcgcgggc
     2041 gtgaagacgc tctaccgaag cgcatccagc ttgcgacgcc tgccgcagcc ctgtgcctct
     2101 ctaacgattg aaagcgatga cgggcgcgaa tgtcaagaaa gccgttagcg attagctgtg
     2161 agccgttagc tgaaaaccag aacgtagacg ggcattcgtc aatgcccgcc cgtcatctgg
     2221 aaccaatggc taacagttaa ccgctaagag ctaacagctt agttaatcgc cgtcgaggct
     2281 agctcgccgg tgggagtttc gacgagggag agaggtgtgc cgcccgtgcc cgtgtggcgg
     2341 cggcgttgtt tgcggtcaga cttaacggag tacatggcct gatctgcggc gatgagcaat
     2401 tgctggagcg tctcgccgtg tgcgccgaag cgcgccgagc cgacgctgat gccgacgcgc
     2461 gcgcgcttgt cgggcgggac gcgcagcgac agccccgcga tggctgcttc gatgcgcccg
     2521 cgcagatcgt caacctgcgc gtgggagagg tcttgcacga gagcgacaaa ctcgtcgccc
     2581 gcgtagcgcg cgaggaaatc atactcgcgc agttgcccgt tcaggacgcg cgccacttcg
     2641 cgcagcattt gatcgcccgt actgtggccg taggtgtcgt tcacgatctt gaagtcgtcg
     2701 aggtcgagca tgacgacctg aaaagaggtg ccgttgcgac gcgcgcgggc ttcctcttcg
     2761 gcaaagcgca ggtgcagggc gcgtgcgttc ggtaaactcg tgagcgcgtc tgtgagcgcg
     2821 ttcgattcgg cttcggcgtg ctgcatggcg ttcgagaaag cgtcggaggc gagccgcgcg
     2881 acggtgtcga gcaggtgcag gtagtcgtcc gtgtactggc ctgacttgag cgcgtagacg
     2941 gccagcacgc cgagcaggcg ttcgtctttg atgagcggca gggcgatcat cgaaccgtag
     3001 ttttcctcct ccggcaattt gaacgcggcg aaatccagca tcggatcgaa gcccgtggcg
     3061 ggacgccggt tggcgatgac gaagccgatc acgccttcgc ccggcgcgac acaatggccg
     3121 cgcaggagtt ccgcgttcag gccgctcacg tgcgcgacgg tcgcgtagtt tttctcttcg
     3181 tcgaagagat agacggcgca ggtgtcgaac ggaacgatat gcccgacctt gttgacgaga
     3241 atctggctgc ggtctgcgat ctccagcgac gcgccgaagg tgctcgcgat ctcgtagagc
     3301 gcgtagactt cgcggtgtgc gctcttgatc tgttcgagat aggcgggcac tgtgtagcag
     3361 ttctggttga tcgatggcgg ctgcgtcgcc gccgcgccgc tcgcttccac gagggcgcgc
     3421 ggttcggctt cgcggctcgt gaagccgtgc tggtcaagcc cgcgctcgac aacctccgct
     3481 tcgaattccg gcaggtgttt gagaaacagc gcaacgactt tcggatcgaa gtgtgtgccc
     3541 gcgccgcgca ggagcaccgc gctcgcctct tcaaccgtca tcccgcgacg atacgggcga
     3601 tcctcgcgca ccgaatcgaa gcaatcgacg acggcgaaga tgcgcgccgt ccagggaatc
     3661 tcttcaccgc gcaggccgtc cgggtagccg aggccgtccc agcgttcgtg atgatggcgc
     3721 acgatgggga cgatggggta agggtaatcg atgcgttcga
//
LOCUS       DASWNS010000589         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_307439, whole genome shotgun sequence.
ACCESSION   DASWNS010000589 DASWNS010000000
VERSION     DASWNS010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307439"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1935
                     /locus_tag="VGB76_20235"
     CDS             <1..1935
                     /locus_tag="VGB76_20235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEY0101263.1"
                     /translation="NNLGGFASATITGANTRRYHGLLTAALRPPTGRVLLLSKFEETL
                     ILDGERFELSTNLYQPGVVHPRGFQQQTDFRLDPFPVFTYQVGDTRIEKVVFMPHGEN
                     ATVVRYRVQASGAAGASLEIRPLVAFRDYHSLRREAPGSFLQAELKPHLVSLSKPDDG
                     SQLLLAHNAVQATIEGQWYRNFEYPEERARGFDCYEDLFNPCLLVWQLEQDATCDLVA
                     SATIEHAPGDAAILEESERTRRRGVTTSTVTAGKGTAGDGEEDTFAHTLVAAADQFLV
                     RRGEGLSSAIAGYHWFTDWGRDTMISLNGLAYARGEFDTAREILLAFASHMREGVIPN
                     RFPDAEEEPEYNNVDGTLWFVNAIGELVRRQPGDIEFVREHLYLCLKEFIEWHERGTI
                     YSIHADEDGLLAAGQEDAQLTWMDAKISDYVVTPRAGKAVEIQALWYNALRTIEEIAA
                     LLRDDWMCNHCRALAELARASFHGLFWNEAGNYLYDYINPAGEPDAAIRPNQIFALSL
                     PHPIVTGSRGRAIVETVERELLTPYGLRSLSPRHADYRPHYEGDAYARDTAYHQGTVW
                     AWLIGPFINAYLRVHGRTPASLAQARLYVRAFREHLREAGVGQISEIFDGDAPHTPRG
                     CIAQAWSVAELLRCELEELN"
     gene            2045..2209
                     /locus_tag="VGB76_20240"
     CDS             2045..2209
                     /locus_tag="VGB76_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101264.1"
                     /translation="MPTATPPPYFPQNPLLPPDENRAKKVKLALFSCLLLLLLFVAAI
                     IGALIYFFGR"
     gene            2336..3043
                     /locus_tag="VGB76_20245"
     CDS             2336..3043
                     /locus_tag="VGB76_20245"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101265.1"
                     /translation="MDKTFQRESRRENRRSFLRQASIFAIALPAGIYIGCARPAQTQP
                     ALASPVESGERIGGYCEGCEAIYEGMPAQLNWETRIAATEPGEPLEASGVIYQPDGKT
                     PARGVILYAYHTDARGIYPPAPDARGMARRHGRLRGWIKTNARGEYKFVTIKPASYPN
                     STIPSHVHLIVKEADKNEYYIDDFEFEDDRFLTQQERARRRKAGGSGVVSLTKNAGGV
                     WLARRNITLGLNVQNYR"
BASE COUNT          662 a         1035 c          955 g          577 t
ORIGIN      
        1 aacaatctcg gcggcttcgc ctctgcgact atcacgggcg caaacacgcg ccgctatcac
       61 ggcctgctca ccgccgcact ccgcccgccc accgggcgcg tcctgctgct ctccaaattc
      121 gaggagactc tcattctcga cggggagcgt ttcgagctat ccaccaatct ctatcaaccg
      181 ggcgtcgttc acccgcgagg atttcaacaa cagacggact tccggctcga cccgttcccc
      241 gtcttcactt atcaggtcgg cgacacgcgc atcgaaaaag tcgtcttcat gccgcacggc
      301 gaaaatgcga cggtcgtgcg ttatcgcgtg caggcgtcgg gcgcagcggg cgcgtcgctt
      361 gagattcgcc cgctcgtcgc cttccgcgat tatcattcgc tgcgccgcga agcccccggc
      421 agtttcctgc aagccgaact caaaccgcat ctcgtcagcc tgtccaaacc ggacgacggc
      481 tcacaacttc tgctcgcgca caacgccgtg caggccacca tcgaaggcca gtggtacaga
      541 aatttcgaat atccggagga gcgcgcacgc ggcttcgatt gctacgaaga ccttttcaac
      601 ccgtgcctgc tcgtctggca gcttgaacag gacgcgacct gcgacctcgt cgcttcggcc
      661 actatcgaac acgctcccgg cgacgccgcc atcctcgaag agagcgagcg cacacgtcgt
      721 cgcggcgtga cgacaagcac ggtcacagcg ggcaaaggca cggcgggcga cggcgaagaa
      781 gataccttcg ctcacacgct tgtcgcggcg gctgatcaat ttctcgtccg gcgcggcgaa
      841 ggtcttagtt cggcgattgc cggttatcat tggttcacgg actgggggcg cgacacgatg
      901 atctcgctca acggcctcgc ctacgcgcgc ggagagttcg acacggcgcg tgaaattctg
      961 ctcgccttcg cttcgcacat gcgcgagggc gtgatcccga atcgcttccc cgatgcggaa
     1021 gaagaaccgg agtataacaa cgtggatggc acgctctggt tcgtcaacgc catcggcgaa
     1081 ctcgtgcggc gacaacccgg cgacatcgag tttgtgcgcg agcatctcta cctgtgcctc
     1141 aaagagttca tcgagtggca cgaacgcggc acgatctatt cgattcacgc ggacgaagac
     1201 gggctgttgg cggcagggca ggaagatgcg cagttaacgt ggatggacgc caagatcagc
     1261 gactacgtgg tgacgccgcg cgccgggaaa gccgtggaga tacaagccct gtggtataac
     1321 gcgctccgca ccatcgaaga gatcgccgcg ctgttgcgcg acgattggat gtgcaatcat
     1381 tgccgcgccc tcgccgaact ggcgcgcgcc agtttccacg ggctgttctg gaacgaagcg
     1441 ggcaactacc tctacgatta catcaatccg gcgggcgaac ctgacgcggc gattcggcca
     1501 aaccagatct tcgctctcag tttgccgcac ccgattgtca ccggaagccg gggtcgtgca
     1561 atcgtcgaga cggtcgagcg cgaactgctc acgccctacg ggttgcgttc gctctcgccg
     1621 cgccatgcgg actatcgccc gcactacgaa ggcgacgctt atgcgcgcga tacggcctat
     1681 catcagggaa cggtctgggc gtggctcatc ggtcctttca tcaacgctta cctgcgcgtg
     1741 catggacgca cgcccgcgag cctggctcaa gcgcgccttt acgtgcgagc tttccgcgag
     1801 cacctgcgtg aggcgggcgt cggacaaatc tcggaaatct tcgacggcga tgcgccgcac
     1861 acgccgcgcg gctgcatcgc gcaggcttgg agcgtcgccg aacttctgcg ctgcgagttg
     1921 gaagagttaa actgaagttc cgagtccgaa gtctcaagtc cggggtcgaa ggcgagtttt
     1981 cgacctgaga cttcggactt aaaaactttc ccgcaccctt ctccaatcca gaggaacaat
     2041 tcaaatgccc accgcgacgc cgccacccta tttcccacag aacccgctcc tgccgcccga
     2101 tgagaatcgc gcgaagaaag tgaagctcgc actcttcagc tgtctcctcc tcctgctcct
     2161 cttcgtcgcg gccatcatcg gcgcgctcat ctattttttc ggaaggtaac gcacggcgaa
     2221 atgtaagccg gagagcgaaa gggagtcgcg gagcgcgcgg ctaaaatttt tggaaggaaa
     2281 ccggcctctg cgtcgtctga tagttggacg actactgtgc gaaagagagg ttgatatgga
     2341 caagacattt cagcgcgaat ctcgacgcga gaatcgccgc agtttcctca gacaggcatc
     2401 catctttgct atcgcgctcc ccgccggaat ctacatcggt tgcgcccgcc ccgcgcagac
     2461 gcagcccgcc cttgcctcgc ctgtggagag cggggaacga atcggcggct attgcgaagg
     2521 ctgcgaggcc atctacgagg gcatgcccgc gcagctaaac tgggagacga ggatcgccgc
     2581 cacagagccg ggcgagccgt tggaggcgag cggcgtgatc tatcagcccg acggcaagac
     2641 gcccgcgcgc ggcgtgattc tctacgccta tcacacggac gcacgcggca tttacccgcc
     2701 cgcacccgac gcgcgcggca tggcgcgcag gcacgggcgt ctgcgcggct ggatcaaaac
     2761 gaacgcgcgc ggcgagtata aattcgtcac gattaaaccc gcgtcatacc cgaacagcac
     2821 catcccttcg cacgtccacc tgatcgtgaa agaggccgac aagaacgaat actatattga
     2881 cgatttcgag tttgaagacg accgctttct cacgcagcag gaacgcgcgc gccgtcgcaa
     2941 ggccggaggc tcaggcgtcg tcagcctgac gaagaacgcg ggcggcgtct ggctcgccag
     3001 acgcaacatc acgctcggcc tcaacgtgca gaactacaga taaagctcgt cgctcctccg
     3061 ctcgaaccgg gttgagacgg aagcgcggcc tcttattttg tcacgcgtcg tacgacgacg
     3121 atgacggcga gcagcgtggt aacggctgcc gagagcaggc agtattgaca gaaggcttcg
     3181 aggacgaagg cttgcacgta gagcagccag aggctcgtag cgaacatga
//
LOCUS       DASWNS010000590         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_462904, whole genome shotgun sequence.
ACCESSION   DASWNS010000590 DASWNS010000000
VERSION     DASWNS010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462904"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VGB76_20250"
     CDS             complement(<1..256)
                     /locus_tag="VGB76_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101266.1"
                     /translation="MHLTTIGFVAALLVLPLASHAFQSNAPQPANAQPAATVRFTDVT
                     EESGVRFKHVSTPEKRYIVESMSGGVALLDYDNDGYLDIYF"
     gene            complement(279..2507)
                     /locus_tag="VGB76_20255"
     CDS             complement(279..2507)
                     /locus_tag="VGB76_20255"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101267.1"
                     /translation="MYLRSTFLPLLLLLLFTHSQVAGDSFRKHHEAAEAARRAGNLEA
                     AEGQYTAILSIAYPALGRLYTAQGNYRGAVAALESAAAHRPDSPDVLVALAIAHFYAG
                     QYKQAAEPLARVLARDARSAPARHMLGKTHFMSGDYAGAARELEAALALSPADHDAAY
                     TLGLAYLKQRQLDEARRVYERLVVALGDRPQLRVLTGRAYRETGFLLEAIAEFKKAAA
                     LDPRFPRVHYHLGLTYLLKDGAAALADAARELQLELAAHPDEFLANYYLGVIHASEGK
                     WAEASRLLEKASRLQPQNPDPFFFLGQAYQNSAKYEQAIAAFREAIRLNPRLEHNDYQ
                     VTNAHFRLGQSLLKAGRTAEGERELELAAELKSKAFKTDEAKLDAFLKTANADAQNKL
                     PELVTAQGVVAGGSADAPDARTREALNADAAFYEKVLAATHNNIGLLRAERQDFRAAA
                     THFDAAAKLNPRHEGLDYNLGLAHFKSGSYKEAVAPLAREVSARPGNLQAKQLLGLSS
                     LMTDDYAKAAALLTEVVAARPGDVTLYYPLALALGKLGRTAEAERITRQMVALGGNSP
                     QVHILLGRAHYEQNDAAKALEELRAALALDPRVRLAHFYSGLIYLKTGKLDEAAREFE
                     AELNLNPTDPEAKYHLAYVALARQETERGIRLMREVISDRPDFANARFELGNALLRQG
                     DTRGAVESLEAAARLSPGQAHIHYQLGRALIAAGRQAEGEGQLDIARQLKDKAMQRTT
                     NP"
     gene            complement(2610..>3291)
                     /locus_tag="VGB76_20260"
     CDS             complement(2610..>3291)
                     /locus_tag="VGB76_20260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101268.1"
                     /translation="NRLAFQTAYTWSHTISNVPTQSYISQTTDVFNYDLDRGDADLDR
                     RHSLVLNAVYALPSFDRWGWFGNHVLGGWQLNGIASFYSGTPLNIFSGQDTAGLGGAG
                     SQRPNRVPGVDLYVDGDDPLQIINPLAFSRPAQGTIGSLGRGSVRGPGIRNVDFSLAK
                     NWQLQERLNLQFRAEMFNVFNFVNFRAHNLSVAGGGIENNFSNAGFGRATSTRGPREI
                     QFGLKLNF"
BASE COUNT          589 a          945 c         1104 g          653 t
ORIGIN      
        1 cgaagtagat gtctagatag ccgtcgttgt cataatcgag gagcgcgacg ccgccgctca
       61 tggattcgac gatgtaacgc ttctccggcg tggagacgtg tttgaaacgg acgcccgatt
      121 cctccgtgac atcggtgaag cgaacagtgg cggcgggttg agcgttggcg ggctgtgggg
      181 cgtttgactg gaaggcgtgc gacgcgagcg gcaggaccag caaggcggcg acgaaaccta
      241 tggtcgttaa atgcaaacct actttacccc gttcgagttt atggattcgt ggtgcgctgc
      301 atcgccttgt ctttcagttg gcgtgcgatg tcgagttggc cttcgccttc cgcctgccga
      361 ccggcggcga tcagggcgcg accgagttga taatgaatgt gcgcctgacc gggcgagagc
      421 cgcgccgccg cttcgaggct ttcaaccgcg ccgcgcgtgt cgccctgccg gagcagagcg
      481 ttgccgagtt cgaaacgcgc gttggcgaaa tcgggtcggt cgctgatgac ttcgcgcatg
      541 aggcggatgc cgcgctccgt ctcctgacgc gcgagcgcga cataagccag atgatatttg
      601 gcctccgggt cagtcgggtt gaggttgagt tcggcttcaa actcgcgcgc cgcttcgtcg
      661 agctttccgg tcttcaagta gatcaggccg gagtagaaat gcgcgaggcg cacgcgtggg
      721 tcgagcgcga gcgcggcgcg caactcttcg agggctttcg ccgcgtcgtt ctgctcgtag
      781 tgcgcgcgac cgagcaggat gtgaacctgc gggctgttgc cgccgagagc caccatctgc
      841 cgcgtgatgc gttcggcttc tgcggtgcgc cccagtttgc cgagcgcgag cgcgagcgga
      901 tagtagagcg ttacgtcgcc gggacgggcg gccaccactt cggtcaggag cgcggcggct
      961 ttcgcgtagt catcggtcat caaggaactc agacccaaca actgctttgc ctgaagatta
     1021 ccggggcgcg ccgacacttc gcgtgcgagc ggcgcgacgg cctctttgta agagccggac
     1081 ttgaaatgag ccagaccgag attatagtcg agtccttcgt ggcgcgggtt gagtttggcc
     1141 gccgcatcga agtgggttgc cgccgcgcga aagtcctgcc gctcggcgcg cagcaaaccg
     1201 atattattat gagttgcggc gagcaccttt tcgtaaaagg ctgcgtctgc gtttaacgct
     1261 tcccgcgtgc gtgcgtcagg cgcgtccgcg cttcctccgg cgacgacgcc ttgcgccgtg
     1321 accaactccg gcaatttgtt ctgcgcgtcc gcgttggcgg tcttgaggaa ggcgtcgagt
     1381 ttcgcttcgt ccgtcttgaa ggctttcgac ttgagttcgg cggcgagctc caactcgcgc
     1441 tcgccctccg ccgtgcgtcc cgctttgagg agcgactggc cgaggcggaa atgtgcgttc
     1501 gtcacctggt aatcgttatg ttccagacgc gggttcaacc ggatggcctc tctgaaagcc
     1561 gcaatcgcct gctcgtattt cgcggagttc tggtatgcct gaccgagaaa gaaaaaggga
     1621 tcgggattct gcggctggag acgcgaggct ttctccagaa ggcggctcgc ctccgcccac
     1681 ttcccttcgc tcgcgtggat cacgccgaga taatagttag cgagaaactc atccgggtgc
     1741 gcggccaact caagttgcaa ctcccgcgcg gcgtctgcga gcgcagccgc gccgtccttg
     1801 agtagatagg ttaggccgag atgataatgg acgcgcggga agcgcggatc gagtgcggca
     1861 gctttcttaa actccgcgat ggcttcgagc agaaaccccg tctcacgata agcgcgaccc
     1921 gtgagcacgc gaagttgcgg gcggtcgccg agagccacga cgagtcgttc gtagacgcgt
     1981 cgtgcttcgt cgagttgacg ctgcttgaga taagcaaggc cgagcgtgta ggccgcgtcg
     2041 tgatctgcgg gcgagagcgc gagcgcggct tcgagttcgc gtgctgcccc ggcatagtcg
     2101 cccgacatga aatgggtctt gccgagcatg tggcgtgcgg gcgcgctccg cgcatcgcgt
     2161 gcgagcactc tggcgagcgg ttcggcggcc tgtttgtatt gccccgcgta gaaatgagcg
     2221 attgccagtg cgacgagcac gtcgggagag tcggggcggt gcgcggcggc tgactccaag
     2281 gctgcgactg cgccgcgata gtttccctgc gcggtataga gccttccgag cgcgggatac
     2341 gcgatggaga ggatggccgt gtactggcct tccgccgcct ccagattgcc cgcgcgccgc
     2401 gcggcctccg ccgcctcatg atgtttccgg aaagaatctc cggcaacttg tgagtgggtg
     2461 aagaggagca gcagcaggag cggaaggaag gttgaacgca aatacatctc taccctgaac
     2521 gctttcgccg gattcgaaaa aaaccgcgac ggaggataac agctaaatca ctgtcgtcct
     2581 ccgtccgggt tcaacttggc cgcactatat cagaagttga gtttcagacc gaactgaatt
     2641 tcgcgtggac cacgcgtgga agtagcccga ccgaagcctg cgttgctgaa gttgttctcg
     2701 atgccgcccc cggcgacgct caggttatgc gctctgaagt tgacgaagtt gaagacgttg
     2761 aacatctctg cgcggaactg tagattgaga cgctcctgaa gctgccagtt cttcgccagt
     2821 gagaaatcga cgttgcggat gccgggaccc ctgaccgaac cgcgaccgag cgagccgata
     2881 gtgccctgcg ccggacggct gaacgccagt ggattgatga tctggagcgg gtcgtcgcca
     2941 tccacgtaaa ggtctacgcc ggggacgcgg ttcggacgct ggctgcccgc gccgccgagt
     3001 ccggcagtgt cctgtccgga gaagatgttg agcggcgtgc cggaatagaa agaggcgatg
     3061 ccgttcagtt gccagccgcc caggacgtga ttgccgaacc agccccaacg gtcgaacgac
     3121 ggcagggcgt agacggcgtt caagaccagc gagtggcgac gatcgaggtc ggcatcgccc
     3181 ctgtcgagat cgtagttgaa aacgtctgtc gtctggctga tgtacgactg cgtcgggacg
     3241 ttactgatcg tgtgcgacca cgtgtaggcg gtttggaagg cgagccggtt g
//
LOCUS       DASWNS010000591        21215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_411739, whole genome shotgun sequence.
ACCESSION   DASWNS010000591 DASWNS010000000
VERSION     DASWNS010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 21215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21215
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411739"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2603)
                     /locus_tag="VGB76_20265"
     CDS             complement(<1..2603)
                     /locus_tag="VGB76_20265"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0101269.1"
                     /translation="MPRVVRHLLAMLVVLLCVALPASAQSTTGSISGTVQDEKEAALP
                     NATVTARNVETNISRTAQTNDEGRYSFVNLPVGAYEVTVEAANFSKYVQTGITLLLNQ
                     RAVVDVTMKLGGVSEIVTVTENAALLNTTNAEVSTRFDSKRLSELPIATNRSVYNVAL
                     SAPGVSQTVTGQSQFASGIGFSANGGRTRSNNFMIDGQDNNDFGVAGATVGLNNPDLI
                     QEVRLVTNQFSAEYGRNGSAVFNAITKSGTNEYHGSAFLFHNDNALNACNNLEKASGF
                     CGTPGAANFKSEKRPFRVENQAGFTVGGPLHLPRFGEGGPAIISGRDKTFFFFSLQRW
                     WDRQLGSGFTINGAPDAAGRAALQAGAGNRPQVAALLKFLPVANGACTGSAAVCAPAT
                     FTVNGQTFTVPKGSLTGSTSSALNDWQQSIRIDHNFSDKHSLFGRYIFQDQDQTGSGQ
                     ATPPGLSQNTLMRSQAVNVSLNSLLTSRVVNELRAAYLRQGQLFTGADPAASEIPSVE
                     ISALGLSGFNAAINRTAIGLAVNFPQFATRQTYQLQDNLSISQGNHNWKFGVDIRRNQ
                     LKQFFVPTIRGRLAYGSLQRFIDDIADSASVNKPLAGGQEITYYDWHDVFAYGMDEWK
                     IRPSFTLTLGLRYESPGQPIGDLAQINDRIVQAAGGDERFRFTPVPKRDNNNFQPRIG
                     FNWNPRTSESGILGFLTGGDKMVIRGGYAVTHDYAFTNIASNIASAFPFLAAVDLPTT
                     PQAGGPGVANAFTTLPTIQGATGLNPNRLVRTIVAEDFRSPYYQQFSLETQRELTRDL
                     VMRVGYVGTKGTALFQSIDGNPRRIGTATCAESDADTCRVNQIQGPIRLRANAASSVY
                     HSMQLSLEKRL"
     gene            3055..3732
                     /locus_tag="VGB76_20270"
     CDS             3055..3732
                     /locus_tag="VGB76_20270"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0101270.1"
                     /translation="MTIRPDPSQLPPPVYDACASHYDRAMRPLERLLLRRLRAATLRA
                     LTADSIGSHPANDGKERDAHGGLRLLEIGAGTGLNFAHYPPGARGAATEPSREMLRRA
                     REKSPPPGLHLVQSRAEALPFADDTFDAAFATLVFCSVASPPEAFAELRRVVRAGGHV
                     VLLEHVRPAGRLAGRLFDALSLLTVRLFDDHFNRRTAEEAGRAGLHLLRVEPHALGII
                     QLIVCEV"
     gene            3815..4690
                     /locus_tag="VGB76_20275"
     CDS             3815..4690
                     /locus_tag="VGB76_20275"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0101271.1"
                     /translation="MTADEKTNTQRDAPDCAQIGCQGWNYEDWVTGPAADETVFYPRG
                     TRPVQMLRMYTRAYETVEVDSTFYAVPSAATVDGWHKRTPPRFTFALKLPQQITHEFG
                     LRKGSSELLAEFCDHARLLHDKLAAVLIQLPPQFEAVPENLRALENFLPRLPRDMPFT
                     IEFRHPGWITDEVLNLLASHRVPVALVEGQWIARRTLFRTVEQITTADFAYIRFMGAR
                     DLTRFDLIQRPQDANLQLWSEAITRLCARVARVYAYFSNYYEGHAPASANKLRRLLNQ
                     PATSPAELENQPSLF"
     gene            4900..5931
                     /locus_tag="VGB76_20280"
     CDS             4900..5931
                     /locus_tag="VGB76_20280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0101272.1"
                     /translation="MSKVARRLSWESGEGLRQKRHRALAYGSYLSHRRQLKRLRREIT
                     AGRKLIVVSLLDRMGDIVAAEPVSRYVRQRSPQAHILWCVREPYRELIDANPHIDETL
                     PALCMTEWGHLARSGLFDEIIDLHIQGRICSTCAQPLVKTSGSPHVNFDNYYHLGNLL
                     NVFCQAAGIPVIDDAPRVYIPQGAASTVDALRLPERFITVHAQSFESGRDWPAAKWRE
                     LVQRVSANWKLPVVEIGIHPTIKLPAPHLIDLCGKLSIMETAEVIKRSSAFVGIDSGP
                     AHLGNALGTPGVILLGRFHLFDRYTPYSGAYADGSGARLIYSAGAASEIPVEQVYEAL
                     REQLKLTQV"
     gene            complement(6088..6393)
                     /locus_tag="VGB76_20285"
     CDS             complement(6088..6393)
                     /locus_tag="VGB76_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101273.1"
                     /translation="MSEHSPPDNNAPVAPAAAASRDLDFEQARLAYSIIASLLEHTQV
                     VSDLVALMAQVIDEDTTRALTQTPNWTAYLESRRRMERTRADVEKFTEILRRLGEDA"
     gene            complement(6390..7613)
                     /locus_tag="VGB76_20290"
     CDS             complement(6390..7613)
                     /locus_tag="VGB76_20290"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY0101274.1"
                     /translation="MNEAVEEIASHKLPLEEMHRKLGATFAERDGWMLPASYGDARAE
                     YEAVRGRAGAGLSDLSARGRIEVGGAEAVQFLNGLITNDVKTLAAGAWMHAAFPNVQG
                     RLLASVRVARPREEVSFLIETEPATHQKVFQTLNRFTLAGDFRVKDVTAETAQFSLQG
                     ALAGACIESVFGNPVPAPDGEPQGAPLVAAAAVVNWRDSHPLTILLRATQTGTDVYDL
                     ICGRDAASDVWEALTKAGARPVGFDAFEVLRVEAGEPRYGVDVSETNVVLEAGREAEA
                     VSYTKGCYIGQEIIARIHWRGHVAKRLAGLVLADERELDAAGAKVRSLDGKEIGRVTS
                     NVFSPFLGRRIALAILKYDYLQPGTQVLVITGEEERAAQVASLPFTRDSRDTGEGAHA
                     GGTDLNDGDDAETTS"
     gene            complement(7647..8288)
                     /locus_tag="VGB76_20295"
     CDS             complement(7647..8288)
                     /locus_tag="VGB76_20295"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0101275.1"
                     /translation="MNSSEQKRELFAALASEAAACRLCEAMCGRTAVLSERNGSVEAR
                     VMFIGEAPGRQGGDRTRVPFSGDQSGHNFGRYIASINLARERIFITNSVLCNPRKESG
                     ANRKPSTAESLNCAAFLARQITLVDPQVVVTLGAVALAALSRIEYHQFSLKDWAGRVC
                     RWHGRLLVPLYHPSPQVLASHRREDAQLRDYQAVARAIRRSARRSTPGAGQAH"
     gene            complement(8403..9440)
                     /locus_tag="VGB76_20300"
     CDS             complement(8403..9440)
                     /locus_tag="VGB76_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101276.1"
                     /translation="MKLSARLFLPFAFYSLVAVALVASSACTTADNTNANTTATTNVT
                     PPPAGSPSPLNLTVAERPQKIKEQMAARGEQDEAAPTLRFVEPRDGATVTGSTVNVKL
                     ALAGELKGYKPAKDPATGMGNHIHVILDNQPYEAYYNLDQPFELRNVTEGRHTLRVFA
                     SRPWHESYKNTGSFQMVTFTVKGGGDATKPTTTAGGQTMADNRNANAANANANANANA
                     ANANASPAASPREGKEMASSQAGEIDATKPLLTYSRPKGDYKGADADAIMIDFWLTNA
                     KLQGDGGEYRVRYSVNGGEAKFIDTWQPIWLTGWTAGKHTVKVELVDKDGNVVENGGY
                     NSTSRDINVSK"
     gene            complement(9639..10256)
                     /locus_tag="VGB76_20305"
     CDS             complement(9639..10256)
                     /locus_tag="VGB76_20305"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEY0101277.1"
                     /translation="MVSNNIYKPRQFDLSGLNGISDKTLELHFKLYEGYVKETNNLTE
                     RISRFLEDGKVDQDEMPAYSELTRRLGFEYNGMVLHEYYFDNMQRQGAGDPAKDSNFI
                     KAATESFGSYDTWKADFVGIGKMRGVGWAMCYQNPANGRLSNHWITLHETGNVSGFTP
                     VLVMDVWEHAFLLDYAPAERPKYIEAFFSNINWDAVEGRLGTSAA"
     gene            complement(10467..11267)
                     /locus_tag="VGB76_20310"
     CDS             complement(10467..11267)
                     /locus_tag="VGB76_20310"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY0101278.1"
                     /translation="MLIESSVHLPAGFAMLQDMFHAPGAEALVRVLLRWTHFVAGITW
                     IGLLYFFNLVNVPFQKGIDADTKKKVNPDLLSRTLWYFRWGAVVTVLAGLMYYAMYIL
                     KSDVVNANANGANANTWVILIVWMIIGTVTFAIEFFLITRVPALTKDGRVLAAIIAVL
                     VIVMAIIIVYWLNSSLTTPGGDSYASNKTHSIGIGGALGLIMMLNVWGIIWPSQKRII
                     AATAGTGPAAPPELARRAFLASRTNAWLSLPMLFFMATSHGDYIILGK"
     gene            complement(11441..11776)
                     /locus_tag="VGB76_20315"
     CDS             complement(11441..11776)
                     /locus_tag="VGB76_20315"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0101279.1"
                     /translation="MPVIEAETATGTQSIDTEAGRKLVLALEDAGIDILHRCGGNAKC
                     TTCRVEILAGDPGEIGEPERTRLAAETGLAENVRLSCQVRVADDLKVRVLRQASTTNL
                     AAGTRPSED"
     gene            complement(11956..12312)
                     /locus_tag="VGB76_20320"
     CDS             complement(11956..12312)
                     /locus_tag="VGB76_20320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY0101280.1"
                     /translation="MSTATMPTVTLNVTENAATEIKKFMASEEGLPETAGLRVRVVPG
                     GCSGFQYSLNIEEESKQGDHVTDAHGVRLFVDMFSMQYLNGVEIDYVNGVMGSGFTFK
                     NPNATGGCGCGSSFTA"
     gene            complement(12505..13587)
                     /locus_tag="VGB76_20325"
     CDS             complement(12505..13587)
                     /locus_tag="VGB76_20325"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY0101281.1"
                     /translation="MTQISEQSVLDALRKIIDPDLHKDIVTLGFIKDLRIDGGEVSFR
                     IVLTTPACPVKEEMQAAAREHVGAIAGVSAVRVTMDAEVPQGRGFGEKVTMPGVRNII
                     AVSSGKGGVGKSTVAVNIAVALAADGARVGLMDADVYGPNAPLMLGVSASKPEVAGNK
                     LLPVKAHGLSMMSMAILKPGDEPMIFRGPILHGLVRQFLQDVLWGELDYLIVDMPPGT
                     GDVQLSLAQLVPVQGAVLVTTPQEVAVADVRRALRMFETVQVPVLGIVENMSYFVAPD
                     TGTRYKIFGEGGGQKLADAYDVPFLGAIPLGLDVREGGDAGVPVVLSHPDSPQAAAIR
                     HVAEEVARQVSIEAMKPELKVVVTQA"
     gene            13644..15731
                     /locus_tag="VGB76_20330"
     CDS             13644..15731
                     /locus_tag="VGB76_20330"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEY0101282.1"
                     /translation="MINERQLKIVRAACPHDCPDTCAMLVTVEDGRAVRVAGDPLHPV
                     TRGTLCTKVANYEQRTHSPDRIQTPLRRTGAKGAKGEVRFERIGWDEALDEIAGRFRA
                     LAASAEGAETIMPYSYCGTMGFVQSESMDRRFFYRLGASIVDRTICASAGSAGYDATI
                     GARIGTDPERFRDARLIILWGTNTITSNVHLWPFILEAKHKGARVIAVDPRRTRTADN
                     CDEHIALLPGTDAALALGLMHVIFAEGLEDKTYLERHTVGAEELKERAREYPPARVAE
                     ICGIEAETIVRLAREYATTRPAVIRLNYGMQRHAGGGMAVRTVTCLPAVTGAWRDAAG
                     GALLSTSSTFPVNRHALERPDLIPTPRPRTLNMSQLGDALTRLSDPPVRALYVYNSNP
                     AAVAPEQAKVLEGLRRDDLFTVVHEQFMTDTCDYADIILPATTQLEHFDLHKAYGHLY
                     LTVNERAIAPLHEAKPNTEVFRLLAARLGFEEDCFKDSDEEIARQAIDMEHPALEGIT
                     LELLRERGWMRLNVPETFAPFAEGNFPTASGKCELSSERLELQGLPAVAEFIPPRESR
                     QSAPELARRFPLALISPAAHAFLNSSFANLPKQVRQEKRPFVEINPEDAAARRISEGD
                     RVRVSNERGACELTAVVTTRARPGVVVSPSVWWNKLSPGDTNINQLTSQSLTDMGGGA
                     TFYDALVEVELLD"
     gene            complement(15752..16171)
                     /locus_tag="VGB76_20335"
     CDS             complement(15752..16171)
                     /locus_tag="VGB76_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101283.1"
                     /translation="MKRKYLVFALVALLLQTSFGVPSVAAAVAVEKTQAERDAQTLEQ
                     VKVKVARLGVGERARVTVKFKDGAKMKGYISQAKEEEFIVRDRQTDEPRTVLYRDVAR
                     VESNRGHSTARNIAIGTAIGVGSILTVIAVLISSHGD"
     gene            16613..18904
                     /locus_tag="VGB76_20340"
     CDS             16613..18904
                     /locus_tag="VGB76_20340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0101284.1"
                     /translation="MRSTVHTEKVSRRAVELRRHALACALALTFGLCFISSHAFAQKR
                     RTPTPRRTPARALSIPEETLLRIVRAEDERRFDAADLGALLSDANASVRARAALGAGR
                     IGDVRAVAPLLSLLQTDRDERVREAAAFALGETEAGAAAEGLLRIIERKTAASVAETP
                     RVRARAIEALGKIAAALPKAEEERAREIGKSLLVALEFEARPSQQPAQRAANREVALL
                     GLTAVLRARPEDAGRVVALFLDSTDARLRADAANTITRLRAKNANDQLRRLLASDADA
                     VVRANAARALGAAEDKTAFDLLLERAANDRDERVRVSAIRSLGTLKDARAAEPLVKRG
                     ELLLSAYRAAKSANAAARPAELNELLELVTGLSRVVAGTANERALGLLRALREAEQWS
                     TPEAEIAAARIAPGQYLRERPFHQLAFRAPDSRISWQTYAAIAQGLGEIAAVSSGRAG
                     NSVVSVQADAQLALRTMLSDTTTPALAVPDVLTAIAAFKPPDGALVMRAQLAAPDVIV
                     RATAAGQLGELAPDDETTSALAAALNTALRDEMNDAALAILDALAKQKSEPAIAAIKT
                     ALDSPDYLLRRRAAAILQAAQPDAANPDEGRIETVATRNRLPDYRRALSRRASRVVAT
                     VNTDKGAFQLELLPNEAPLTVDNFVELARRGFFNNISFHRVVPNFVVQGGDPRGDGNG
                     GPGYQIRCEINEVPYGRGAVGMALSGKDTGGSQWFITHSPQPHLDGGYTVFARVVTGM
                     ETVDRITRGDRIRSINVAETKHK"
     gene            18999..20918
                     /locus_tag="VGB76_20345"
     CDS             18999..20918
                     /locus_tag="VGB76_20345"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0101285.1"
                     /translation="MSQFIKQTTRIKFGLFVSALLCLVSTLASAQSPRASASSPASQA
                     SKPAPARTRYQINFALDFDARTFSATERVRWVNRDQRPASVVYFHLYANLRSNSNGNN
                     NDANRAPAPPVSGAEATAAATAIAATAVDEPRIEIKSVRHAATQTALAYALDEQATLL
                     RINLREPVAAGATTEIEIAFTGAVPEIDPDETSLPSHVIQQLGAALRDTRETRRARDT
                     NFRARGILMLGSAYPVLAVRNGDEWQREVRATVGDPAHTEVADYEVTIDAPAEVALYT
                     SGEARSLAGGSTVNPDSGSSNVKPGSGADAVKSGARLFAGENLRNFAIIAGRTLASGE
                     RTVGGVRVRSVWTTEHEKIGRRVLDAAAEAVRVFTVRFGALPVTSVTVAEAPLVAGLG
                     SAEFANLSVIASAFYVDFDSPAIRNLPELVREQRASVEDSLEFATAHMLAHQWWGMLV
                     GNSPERAPVLDEALAHWSALVYYRETHGHVRAQAARDDQLRGVYEIYRTFGGDDMPAD
                     RPARDYRNSFQYAAIVSAKGALMYDALVKLLGEERVFTALRRYAEANRFEIAELDDLR
                     GAFIAEAPVAERRAVTRTFDRWLSERHGDEDIAPPNPQLASALGLTPEPAGTPNRNAF
                     SRLGRFFWRQMTRIR"
BASE COUNT         3934 a         6754 c         6519 g         4008 t
ORIGIN      
        1 ctcaggcgtt tctcaaggct gagttgcatc gagtggtaga cggaagaggc cgcgttggcg
       61 cgcaggcgga tcggcccctg aatttgattg acgcggcagg tgtcggcgtc ggattccgcg
      121 caggtggccg tgccgatgcg acgcgggttg ccgtcaatgg attggaacag agccgtgccc
      181 ttcgtgccga cgtagccgac gcgcatcacg aggtcgcgcg tcaactcgcg ctgcgtctcc
      241 agactgaact gctggtagta gggcgagcgg aagtcttcgg cgacgatggt gcgaaccagg
      301 cggttcggat tcagacccgt cgcgccctga atggtcggga gcgtcgtgaa ggcgttggcg
      361 acgccgggac cgcccgcctg cggggtggtc ggcagatcga ctgcggcgag gaacgggaac
      421 gcgctcgcaa tgttggaagc gatgttggtg aacgcgtaat cgtgtgtcac ggcgtagccg
      481 ccgcgaatca ccatcttgtc gccgcccgtg agaaagccga ggatgccgct ctcgctcgtg
      541 cgcgggttcc agttgaagcc gatgcgcggc tggaagttgt tgttgtcgcg tttgggaacg
      601 ggcgtgaaac ggaaacgctc gtcgccgccc gccgcctgca cgatgcggtc gttgatctgc
      661 gcgaggtcgc cgatgggctg tcccggcgat tcgtagcgca ggccgagcgt gagcgtgaag
      721 ctcgggcgga ttttccactc gtccatgccg taggcgaaca cgtcgtgcca gtcgtagtaa
      781 gtgatctcct ggccgcccgc gagaggcttg ttgacggagg ccgaatcggc gatgtcgtcg
      841 atgaagcgtt gcagcgaacc gtaggccagc cgcccgcgaa tcgtcgggac gaagaactgt
      901 ttcaactggt tgcggcggat gtcaacgccg aacttccagt tgtgattgcc ctgcgagatc
      961 gagaggttgt cttgcagttg gtaagtctgg cgcgtggcga actgcgggaa gttgacggcg
     1021 aggccgatgg ccgtgcggtt gatcgccgcg ttgaagcccg aaaggccgag cgcgctgatc
     1081 tcgacggaag gaatctccga ggcggcaggg tctgcgcccg tgaacagttg cccctgacgc
     1141 agatacgcgg cgcgcagttc gttgacgacg cgcgaagtga gcaggctgtt gagcgagacg
     1201 ttgacggcct gactgcgcat cagagtgttc tggctcaagc ccggcggcgt cgcctgtcca
     1261 gaacctgtct ggtcttgatc ctgaaagatg taacgaccga agagcgagtg cttatcggag
     1321 aagttatggt cgatgcgaat cgactgttgc cagtcgttga gcgcgctgga ggtggagccg
     1381 gtcagagagc ctttgggcac ggtgaacgtc tgcccgttga cagtgaacgt ggccggggcg
     1441 cagacggcgg cagagccggt gcaggcaccg ttggcgacgg gaaggaactt gagaagcgcg
     1501 gcgacctgcg ggcggttgcc cgcgcccgct tgcagagccg cccgacccgc agcatcgggc
     1561 gcgccgttga tggtgaagcc cgaaccgagt tggcgatccc accagcgttg gagcgagaag
     1621 aagaagaagg tcttgtcgcg cccgctgatg atggcgggtc caccttcgcc gaagcgcggg
     1681 agatggaggg ggccgccgac ggtgaagccc gcctgattct ccacgcggaa gggtcgtttc
     1741 tcgctcttga agttggccgc gccgggcgtg ccgcaaaagc cgctggcctt ctcaaggttg
     1801 ttacaggcgt tcaacgcgtt gtcgttgtgg aagaggaacg cgctgccgtg atattcgttc
     1861 gtgccgctct tcgtgatcgc gttgaagacg gcggaaccgt tgcgcccgta ttcggcggag
     1921 aactggttgg tgacgaggcg cacttcctgt atgaggtcgg ggttgttgag tccgacggtc
     1981 gcgcccgcga cgccgaagtc gttgttgtcc tgcccgtcaa tcataaaatt gttcgagcgc
     2041 gtgcgaccgc cgttcgcgga gaaaccgatg ccgctggcga actgcgattg gccggtgacg
     2101 gtctggctga cgccgggagc cgagagcgca acgttataga cgctgcggtt ggtagcgatg
     2161 ggcaattccg aaagccgctt ggagtcgaag cgtgtgctga cttccgcgtt cgtcgtgttg
     2221 agcagagccg cattctccgt cacggtgacg atctcgctga cgccgcccag tttcatcgtc
     2281 acgtcaacca ccgccctctg gttcagcaag agcgtgatgc cggtctggac gtacttcgag
     2341 aagttggccg cttcgaccgt gacttcatac gcgccgacgg gtaggttgac gaagctgtaa
     2401 cgcccttcat cgttagtttg agcagtacgc gaaatattgg tttcgacgtt gcgcgccgtc
     2461 acggtggcgt tcggcagcgc ggcctctttc tcgtcctgca cggtgccgga aatcgaaccc
     2521 gtagttgatt gcgccgacgc gggcagcgcc acacacagca gcacgacgag catcgcaaga
     2581 aggtgtctga ctactctcgg catttctttg ctccttatag gctcagagat ggatatgcgg
     2641 ttgaaggcgt ggctactgct gctggtcgcg cgcgaggtgg cgactgtgag tttcgccttc
     2701 cgatttagtg ttcgaataac ctgagttgcg ctttggtaac gcctgtgaca gctaagactc
     2761 tttcgagttc agagtgcttc cagcaaagga cttgcggtac ggattcttct gttaatctta
     2821 gctgtcggga aataatcccg gtgaaggtac gactgatcaa gttcttgtca cgcaagcggt
     2881 atgcccgcag gccacgggcg cgaattgtgg ccgatatgta gccattcggg gctgacaact
     2941 gcgcgctgat ccatattttc gtagactacc cctacaattt tatgcagaac cccgcgcccg
     3001 gcacgcgcac gcaaccagcg cacactccgc ttgcgcaaga gagagagact tcgcttgacc
     3061 attcgacccg acccgtctca actgccgccg cccgtctacg acgcctgcgc atcccactac
     3121 gaccgcgcga tgcgtccgct cgaacgcctc ctgctacgac gcctgcgcgc cgcgacgctt
     3181 cgcgcgctga ccgccgacag catcggcagc catcccgcca acgacggcaa agagcgcgac
     3241 gctcacggcg gcctgcgcct gcttgagatc ggcgcgggca caggactcaa cttcgcgcac
     3301 tacccgcccg gcgcgcgcgg cgcagcgacc gaaccgagcc gcgagatgct ccgtcgcgca
     3361 cgcgagaaat cgccgccccc cggcctccat ctcgtgcaga gccgcgccga agcgttgccc
     3421 ttcgcagacg atacgttcga cgcggccttc gccacgctcg tcttctgctc cgtcgcctcg
     3481 ccgccggagg ctttcgcgga actgcgtcgt gtcgtgcgcg cgggcgggca cgtcgtgctg
     3541 ctcgaacatg tgcgccccgc cgggcgactg gccggtcgtc tcttcgacgc gctcagtctc
     3601 ctcaccgtcc gtctcttcga cgaccacttc aaccggcgca ccgcagaaga agcaggccgc
     3661 gccggactgc atctcctgag agtcgaaccg cacgcgctcg gcatcatcca gctcatcgtc
     3721 tgcgaagtgt gaagacggtt tccggcgtct ctcattttgt gtctgcgcgt ctttgggttt
     3781 aagttatcgg aaagctctac ggagaagagg acgcatgacg gcagacgaaa agacgaacac
     3841 acagcgcgac gccccggact gcgcgcagat cggttgtcag ggctggaact acgaagactg
     3901 ggtgaccgga cccgctgccg acgagacggt cttctacccg cgcggcacgc gcccggtaca
     3961 gatgctacgc atgtacacgc gtgcttacga gacggtcgaa gttgactcca ccttctacgc
     4021 cgtgccctcc gccgccaccg tggacggctg gcacaaacgc accccgccgc gtttcacttt
     4081 cgcgctcaaa ctcccgcaac agatcacgca cgagttcggg ctgcgcaaag gaagctccga
     4141 actcctcgcg gagttttgcg atcacgcgcg cctgctgcac gacaaactgg ccgccgtgct
     4201 catccaactg ccgccgcagt ttgaagccgt gcccgaaaat ttgcgcgccc tcgaaaattt
     4261 cctcccgcgc ctgccgcgcg acatgccttt caccatcgag tttcgccacc ctggttggat
     4321 cacggacgag gtgctcaacc tgctcgcctc gcaccgcgtc cccgtcgccc tcgtcgaagg
     4381 ccagtggatc gcgcgccgca cgctctttcg caccgtcgaa cagatcacca ccgcagactt
     4441 cgcttacata cgcttcatgg gcgcacgcga cctcacacgc tttgacctca tccaacgccc
     4501 gcaggacgcc aacctccaac tctggagcga agccattaca cgactctgcg cccgcgtcgc
     4561 gcgcgtctac gcctatttca gcaactacta cgaagggcac gcgcccgcct ccgccaacaa
     4621 gctcagacgc cttctcaatc aacccgccac ctcgcccgcc gaactcgaaa accagccgtc
     4681 gctcttttga tatttcgggc aacgcgttcg ctccgcgtcg caagcgcagg gcgacgccgt
     4741 cgcgccgtat aacacgagga ttcggagtgt tatactgccg ctcgccgaag cggtctgtgg
     4801 tcttgagccg ggcgttttcg tccggggaag gttcgagcgc gatgagatct ccgaagctga
     4861 cgatggtgaa tctttaaggt gcgcgaagtc tctcggtttt tgagcaaggt cgcacggcgg
     4921 ctcagttggg aatcgggaga ggggctgcgg caaaagcgtc atcgcgcact cgcctacggc
     4981 agttatctct cgcaccggcg acaactcaaa cgtctcagac gcgagataac ggcagggcga
     5041 aagttaatcg tcgtcagcct gctcgaccgc atgggcgaca tcgtcgcagc cgaacccgtc
     5101 tcccgctacg tcaggcagag aagtccgcag gctcacattc tctggtgcgt ccgcgagcct
     5161 taccgcgagt tgatcgacgc caacccgcat attgacgaga cgctgcccgc gctctgcatg
     5221 accgagtggg gacacctggc gagaagcggc ctcttcgacg aaatcataga cctgcacatt
     5281 caaggccgca tctgctccac ctgcgcccaa cccctcgtca agacgagcgg cagcccgcat
     5341 gtcaatttcg acaactacta ccatctcggc aacctcctca acgtcttctg tcaggccgcc
     5401 gggatacctg tcatagacga cgcgccgcgc gtctacatcc cgcagggagc ggcgagcacc
     5461 gtagacgcac tccgtttgcc ggagcgtttc atcaccgtcc acgcgcagtc gttcgagtcg
     5521 gggagggact ggcctgccgc caaatggcgc gaactggttc agcgtgtgag cgcgaactgg
     5581 aaactccccg tcgtcgagat cggcatccac ccaaccatca aactccccgc cccgcacctc
     5641 atagacctct gcggcaagct ctccatcatg gagacggctg aagtgatcaa acgctcctcg
     5701 gccttcgtcg gaatcgatag cggtccagcg catctcggta atgctctggg cacgccgggc
     5761 gtcatactcc tcggcaggtt tcacctcttc gaccgttaca cgccctacag cggagcttat
     5821 gccgacggct ccggcgcgcg gctcatctat tcggcaggtg cggcttcgga gattccggtc
     5881 gaacaggtct acgaagcatt gagggaacag ttgaagctga cgcaggttta gcgagacacg
     5941 acgctccgcg actttcgcgc acgcagcaga gaacgactcc atgccggaat cgcacactag
     6001 cgggcgtcta aaatttacgc acggcgggtc tgttctgtaa gctgaaagcg cttgagttgt
     6061 ggcgaggtta aacgcgctcg cgggaagtta agcgtcctcg ccgagccttc ggaggatctc
     6121 tgtgaatttc tccacgtcgg cgcgggtgcg ttccatccgg cgacggcttt cgagataggc
     6181 cgtccagttg ggcgtctgcg tgagcgcgcg cgtcgtgtcc tcgtcaatga cctgcgccat
     6241 gagcgcgacg agatcgctca cgacctgcgt gtgttcgagc agtgatgcga tgatggaata
     6301 ggcgagccgc gcctgctcga aatcgagatc acgcgacgcc gccgccgccg gggcgacggg
     6361 cgcgttattg tccggcggcg agtgttcgct cacgatgtcg tctccgcgtc gtctccgtcg
     6421 ttgaggtcag tgccgccagc gtgtgcgcct tcgccggtgt cccgactgtc gcgcgtgaaa
     6481 ggaagcgagg cgacctgcgc cgcgcgttcc tcttcgcccg tgatgaccaa gacctgcgtg
     6541 cccggttgga ggtaatcgta tttgagtatc gcgagcgcga tgcggcgacc gaggaacggc
     6601 gagaagacgt tggaagtgac gcgcccgatc tcttttccgt ctaggcttct gacttttgcg
     6661 ccagccgcat cgagttcgcg ttcgtctgcg agtacgagtc cggcgagacg cttggcgacg
     6721 tggccgcgcc agtgtatgcg cgcgatgatc tcctgcccga tgtaacagcc tttggtgtag
     6781 ctgaccgcct ctgcctcccg ccccgcttcg agcacgacgt tcgtttcgct cacatccacg
     6841 ccgtagcgcg gctcgcccgc ttccacgcgc aacacttcaa aggcgtcgaa gccgaccgga
     6901 cgcgcccccg ccttcgtcaa agcctcccac acatccgacg ccgcatcgcg cccgcagatg
     6961 aggtcataaa cgtctgtgcc cgtctgcgtc gcgcgcagga ggattgtcaa cggatgagag
     7021 tcgcgccagt tgacgactgc ggcggcagcg acgagcggcg cgccctgcgg ctctccgtca
     7081 ggcgcgggca cggggttgcc gaagacagac tcgatgcacg cgcccgccag cgcgccctgc
     7141 aaggagaact gcgccgtctc cgccgtcaca tctttgacgc gaaagtcgcc tgcgagcgtg
     7201 aagcggttga gcgtctggaa aactttctga tgcgtggcgg gttccgtttc gatgagaaag
     7261 ctgacctctt cacgcggtcg cgcgacgcgc acgctggcga gcagtcgtcc ctgcacgttc
     7321 gggaaagcgg cgtgcatcca cgcgcccgcg gcaagcgtct tcacatcgtt ggtgatgagg
     7381 ccgttcagga attgcacggc ctccgcgccg ccgacctcga tgcgcccgcg cgcggagaga
     7441 tcagacagcc ctgcgcccgc tctgccgcgc accgcttcgt attccgcgcg cgcgtcgccg
     7501 taagaggccg gaagcatcca gccgtcgcgc tcggcgaagg tcgcgccgag tttccggtgc
     7561 atctcttcga gcggtagttt atggctcgct atttcttcaa ccgcttcatt catgtttcgt
     7621 taaaaagagt ttcggcaaac ttctatctag tgcgcctgtc ctgcacccgg cgtcgagcgg
     7681 cgtgcgcttc gccgtatggc gcgtgcgacg gcctgataat cgcgtagttg cgcgtcttcg
     7741 cggcggtgcg aggcgagcac ctgcgggctt ggatgataga gcggcacgag cagacgcccg
     7801 tgccagcggc agacccgacc agcccaatct ttgagtgaaa attgatgata ctcaatgcga
     7861 ctgagcgcgg caagcgcgac cgctccgagc gtgacgacga cctgcggatc gacgagtgtg
     7921 atctgtcgtg cgaggaacgc ggcgcagttc agagattccg ctgtgctcgg cttgcggttc
     7981 gcgccagact ctttgcgcgg gttgcagagc acggagttgg tgatgaagat tcgttcgcgc
     8041 gcgagattga tcgaagcgat gtaacgcccg aagttgtgcc ccgactggtc gcctgaaaag
     8101 ggaacgcgcg tgcggtcgcc gccctgccgc cccggcgctt cgccgatgaa catcacgcgc
     8161 gcttcgacgc tgccgttgcg ctcactcaag accgccgtgc gcccgcacat cgcctcgcac
     8221 agacggcacg cggcggcctc cgaggcgagc gcggcgaaga gttcgcgctt ttgttcgctt
     8281 gaattcatcc ggctaattta accacacgca gacgcgcggc acgaagaagc gaaatcgatc
     8341 ttcgtgccgc gcgtttacgc gtaactgacc tctgattact tactgacgtt gctgtcgcgt
     8401 gattacttac tgacgttgat gtcgcgcgag gtggaattgt agccgccgtt ttccacgacg
     8461 ttgccgtcct tgtccacgag ttcgaccttg actgtgtgct tgcccgcagt ccagcccgtg
     8521 agccagatcg gttgccaggt gtctatgaac ttcgcctcgc cgccgttcac cgaataacgc
     8581 acgcgatact cgccgccgtc gccttgcagc ttcgcgtttg tcagccagaa gtcgatcatg
     8641 atggcgtcgg cgtccgcgcc cttgtagtcg cccttcggac ggctgtaggt caggagcggc
     8701 ttcgtcgcgt cgatctctcc ggcctgactc gacgccatct ccttgccttc gcgcggcgac
     8761 gccgccggag aggcgttcgc attagcagca ttagcgttgg cgttggcgtt cgcgttggct
     8821 gcgttcgcgt tcctgttatc ggccatcgtc tggccgccgg cggtggtcgt cggctttgtc
     8881 gcatcgccgc cgcctttcac cgtgaaggtg accatctgga aagagcctgt gttcttgtag
     8941 ctctcgtgcc acgggcgcga ggcgaagacg cgcagggtgt gtcggccttc ggtgacgttg
     9001 cgcagttcga acggctggtc gagattgtag taagcctcgt aaggttgatt atcgaggatg
     9061 acgtggatgt gattgcccat gccggtcgcg gggtctttgg cgggcttgta gcctttgagt
     9121 tcgcccgcga gcgcgagttt gacgttgacg gtcgaacccg tgacggtcgc gccgtcgcgc
     9181 ggctcgacga agcgcagggt cggcgcggct tcgtcctgct cgccgcgcgc cgccatttgc
     9241 tccttgatct tctgcgggcg ttccgcgacg gtcagattta gcggcgaggg cgaaccggcg
     9301 ggcggcggcg tgacgttggt ggtggccgtc gtgttggcgt tcgtgttgtc ggcggtcgtg
     9361 caggctgacg aggcgacgag agcgacggcg acgaggctgt agaacgcgaa gggtaaaaag
     9421 agacgtgcgg aaagtttcat ttaactcggc ctcctgaaat gttcgagtga ttgcgggtga
     9481 gagtatagaa agaaaagggg aaaggttaaa gggtacgcag gggaaagggc gaaggggaaa
     9541 acgaaaaggt tgaagagagg cgcgcacgac gaacgcgccg cgtctctttc ccttcgcccc
     9601 ttacccctcg ccctttcccc tcgctctgtg cccttcgctt acgctgcgga cgtgccgaga
     9661 cgcccttcca ccgcatccca attgatattc gagaagaagg cttcgatata tttggggcgt
     9721 tcggcgggcg cgtagtcgag caggaaggcg tgctcccaca cgtccatcac gaggacgggc
     9781 gtgaagccgg agacgttgcc cgtctcgtgc agagtgatcc agtgattcga gagcctgccg
     9841 ttggccggat tctggtagca catcgcccaa ccgacgccgc gcatcttgcc gatgccgacg
     9901 aaatcggcct tccacgtgtc gtaactgccg aaactctccg tcgccgcttt gataaagtta
     9961 gagtctttcg cggggtcgcc cgcgccctgc cgttgcatgt tgtcgaagta gtattcgtgc
    10021 aagaccatgc cgttgtactc gaagccgagc cgtctggtca gttccgagta ggcgggcatt
    10081 tcgtcctgat cgactttgcc gtcttcgaga aaccgggaga tgcgctccgt caaattgttc
    10141 gtctctttga cgtagccttc gtagagtttg aaatgcaact ccagagtctt gtcggagatg
    10201 ccgtttaagc cgctcaagtc aaactgccgg ggcttataga tattgtttga aaccatcttt
    10261 cgtcctcact tttttcgaac gattgcagga ttgtacgccc gacagtctat acctgccagc
    10321 gcgccggtgt gtgacgaatg aatggagaga atgagaagaa taagtgatga gaagaataag
    10381 taatgagtaa tgagtgatgg gtgatgagtt aaagacaaaa gttcttaact catcacccat
    10441 cactcattac tcatcactta ttacgatcac ttgccgagaa tgatgtagtc gccgtgcgag
    10501 gtcgccatga agaagagcat ggggagcgag agccaggcgt tggtgcgcga ggcgaggaag
    10561 gcgcggcgcg cgagttcggg cggcgcggcg ggtcctgttc cggcggtcgc ggcgatgatc
    10621 cgcttttgcg aaggccagat gatgccccag acgttcagca tcatgatcag gccgagcgcg
    10681 ccgccgatgc cgatggagtg ggtcttgttg ctcgcgtagc tgtcgccgcc gggcgtggtc
    10741 agagacgagt tgagccagta gacgatgata attgccatca cgataacgag aacggcgatg
    10801 atggcggcga gcacccgccc gtctttcgtt aaagcgggga cgcgcgtgat gaggaaaaac
    10861 tcgatggcga aagtgacggt gccgatgatc atccagacga tgaggatgac ccacgtgttg
    10921 gcgttcgcgc cgttcgcatt agcgttcacc acgtcgctct tgagaatgta cattgcgtag
    10981 tacatcaaac cggcgagcac ggtgacgacc gcgccccagc ggaaatacca gagcgtgcgc
    11041 gagagcaggt cgggattcac cttcttcttc gtgtcggcgt cgatgccctt ctggaagggg
    11101 acgttgacga ggttgaaaaa gtaaagcagc ccgatccagg tgatcccggc gacgaagtgc
    11161 gtccagcgca acaggacgcg cacgagtgcc tctgcaccgg gggcgtgaaa catgtcttgc
    11221 agcatcgcga aaccggcggg gagatgaacg gatgactcga tcagcatgat gacctccgaa
    11281 ttttcttctt ggttgtggtg tggcttgctc gaacggttgt tacgcgcgag agattaacac
    11341 caagcggaac gaggcgcaat aactatagcg gtgagagatt ggaggccgga gttaatgaaa
    11401 actgtgttct tccaacctcc ggcctgacgg ctgctgacct ttagtcttcg ctcggtctgg
    11461 ttcccgccgc aaggttggtg gtgctggcct ggcgcaggac gcgcactttg agatcgtccg
    11521 cgacgcgcac ctgacaggag aggcgtacgt tttcggccaa gcccgtctca gcggcgaggc
    11581 gcgtgcgttc gggttctccg atctcgccgg ggtcgcccgc gagaatctcc acgcggcagg
    11641 tggtgcattt ggcgttgccg ccgcagcgat gcaggatgtc gatcccggcg tcttcgaggg
    11701 cgaggacgag tttgcgtccc gcttccgtgt caatggactg tgtgcctgtg gcagtctcgg
    11761 cttcgattac gggcatcttg ggctttgctc ctgagagaag attttaagtt gaatgctgtg
    11821 cgggcgagtg tacaccaaaa aacgaaaagc gcggtacgcg aaagtcgaaa cgcgcccgtc
    11881 gcccggttaa aagagcgaag gcgcgcccgc gctttcacgc gaccgcgtct tctctgaaga
    11941 aactattaat gtcgcttagg cggtgaagga agaaccgcag ccgcaaccac ccgtcgcgtt
    12001 cggattcttg aaggtgaagc ccgaacccat cacgccgtta acgtagtcga tttcgacgcc
    12061 gttcaaatac tgcatgctga acatatcgac gaagagacgc acgccgtgcg cgtccgtcac
    12121 atgatcgccc tgcttcgatt cctcttcgat gttgaggctg tactggaaac cggagcaacc
    12181 gcccggcacc acgcgcacgc gcagcccagc cgtctcaggc aaaccctctt cgctcgccat
    12241 aaatttcttg atctcggtcg ccgcgttttc cgtgacgttg agcgtcaccg tcggcatcgt
    12301 tgctgttgac atttttttct ccttaaatta ttgcctgata atcaatatcc gtcgatcaac
    12361 gttctttaga tgtccgttgt ccgtcgtccg ttgttaagag ccaccctgtt cttaatatta
    12421 accgccttgc tccccggcta caagcaaaga acagcggtcg tgaacgattg gcacagaacg
    12481 cccggcaact cacgacgaac gatattaagc ctgcgtgacc accactttga gttcgggctt
    12541 catcgcttca atcgaaacct gccgcgcgac ctcttccgcc acatggcgaa tcgccgccgc
    12601 ttgcggcgaa tccgggtggc tgagaactac gggcacgccc gcgtcgccgc cttcgcgcac
    12661 gtccaagccc agcgggatcg cgccgagaaa cggcacatcg taagcgtccg ccaacttctg
    12721 cccaccgcct tcgccgaaga ttttgtagcg cgtgcccgtg tccggcgcga cgaaataact
    12781 catgttctcg acgatgccga gcacaggcac ctgcaccgtc tcgaacattc ggagcgcgcg
    12841 gcgcacgtcg gccacggcca cttcctgcgg cgtcgtcaca agcaccgcgc cctgcaccgg
    12901 aacgagttgc gccaaactca gttgcacgtc gcccgtgccc ggcggcatgt ctacgatgag
    12961 ataatcgagt tcgccccaga gcacgtcctg taaaaactgg cgcaccaacc cgtgcaggat
    13021 cgggccacga aagatcatcg gctcgtcgcc cggcttcagg atcgccatgg acatcatcga
    13081 gaggccgtgc gctttgacgg gcaggagttt gttgcccgcc acttccggct tcgacgcgct
    13141 gacgccgagc atgagcgggg cgtttggtcc atagacatcc gcgtccatga ggccgacgcg
    13201 cgcgccgtcc gccgcgagcg cgacggcgat gttgaccgcc acggttgact tgccgacgcc
    13261 gcctttgccc gaagagactg cgatgatgtt gcgcacgccc ggcatcgtca ccttctcgcc
    13321 gaagccacgc ccttgcggca cttccgcatc catcgtcacg cgcacggcag agacgcccgc
    13381 aatcgcgccg acgtgttcgc gtgcagccgc ctgcatctct tctttcaccg ggcaggccgg
    13441 ggtcgtcaag acgatgcgaa acgaaacctc gccgccgtca atgcgtaaat ccttgatgaa
    13501 tccgagcgtg acgatgtcct tgtgcaagtc cggatcgata attttgcgca gggcgtcgag
    13561 cacgctctgt tccgatattt gtgtcataat tgccatccgg taaattatac aaggtattcg
    13621 cgcccggcgt acaattgagc ggcatgatta acgaacgaca actcaaaata gttcgggcgg
    13681 cctgcccgca cgattgccct gatacgtgtg cgatgctcgt gacggtggag gacgggcgcg
    13741 ccgtgcgcgt cgcgggcgac ccgttgcatc ccgtcacgcg cggaacgctc tgcaccaaag
    13801 tcgccaacta cgaacagcgc acgcacagcc ccgaccgcat tcaaactccg ctccggcgca
    13861 cgggcgcgaa aggcgcgaaa ggtgaagtgc gattcgagcg catcggttgg gacgaagcgt
    13921 tggacgagat cgcaggtcgt ttccgcgcgc tcgccgcgtc cgcagaaggc gcggagacga
    13981 tcatgcctta tagttattgt ggcacgatgg gtttcgtcca atccgaatcg atggaccggc
    14041 gtttcttcta tcgtctcggc gcgtccatcg tcgaccgcac gatctgcgcc tccgccggtt
    14101 ctgccggata cgacgccacc atcggcgcac gcatcggcac agaccccgaa cgcttccgcg
    14161 atgcgcgcct catcatcctg tggggcacga acaccatcac gagcaacgtg catctctggc
    14221 cgttcatcct cgaagcgaaa cacaagggcg cgcgcgtcat cgccgtcgat ccgcgccgca
    14281 cacgcaccgc cgacaactgc gacgaacaca tcgcgctcct ccccggcacg gatgctgcgc
    14341 tcgcgctcgg cctgatgcac gttatcttcg cggaaggctt ggaggataaa acctatcttg
    14401 aacggcacac cgtcggcgcg gaagagttga aggagcgtgc gcgtgaatac ccgcccgcgc
    14461 gggtcgcgga gatttgcggc atcgaagcgg agacaatcgt gcggctcgcg cgcgagtatg
    14521 cgacgacgcg ccccgccgtc atacgcttga actacggcat gcagcgacac gcgggcggcg
    14581 gcatggccgt gcgcaccgtc acctgcctgc ccgccgtaac cggcgcatgg cgcgacgcgg
    14641 cgggcggcgc actgctctcc acaagcagca ccttccccgt caaccgtcac gcgctcgaac
    14701 gccccgatct cattcccacg cctcgcccgc gtacgctcaa catgtcgcaa ctcggcgacg
    14761 cgttgacacg tctgagcgat ccgcccgtcc gcgcgctcta cgtctataat tccaacccgg
    14821 cggcggtcgc gcccgaacag gcgaaggtgc tcgaaggttt aaggcgcgat gatctgttca
    14881 cggtcgtcca cgaacagttc atgacggaca cctgcgacta cgcggacatc atcctgcccg
    14941 cgaccacgca gcttgagcat ttcgatctgc acaaggctta cggccatctc tatctcaccg
    15001 tcaacgagcg tgcgattgcg ccgctccacg aggcgaagcc gaacacggag gtctttcgcc
    15061 tgctcgccgc gcgcctcggc ttcgaggaag actgtttcaa ggattccgac gaagagatcg
    15121 caaggcaggc gattgacatg gagcatcccg cgctcgaagg cattacgctc gaactcttgc
    15181 gcgaacgcgg ctggatgcgt ctgaacgtgc cggagacttt cgcgccgttc gccgaaggaa
    15241 acttcccgac cgcctccggc aagtgtgagc tttcgagcga gcgtctcgaa ctccagggct
    15301 tgcccgccgt cgcagagttc atcccgccgc gcgagtcgcg gcagagcgcg ccggaactcg
    15361 cgcgtcgttt cccgctcgcg ctcatcagtc cggcggcgca cgcgtttctc aattcgagtt
    15421 tcgccaatct cccgaagcag gtcaggcagg agaaacgtcc cttcgtcgag atcaacccgg
    15481 aggacgccgc cgcgcgccgc atctcggaag gcgaccgcgt gcgcgtctcg aacgaacgcg
    15541 gcgcgtgcga actgacggcg gtggtcacga cgcgcgcacg ccccggcgtc gtcgtctcgc
    15601 cttccgtctg gtggaacaag ctctcgcccg gcgacacgaa tatcaaccag ttgacttcgc
    15661 aaagcctgac cgacatgggc ggcggcgcaa ccttctacga cgcgctcgtc gaagtcgaac
    15721 tgctggattg aagcgcagaa caggttgacc gttaatcgcc gtgcgaggag atgaggacgg
    15781 cgatcacagt taagatcgaa ccgactccga tggccgtgcc gatggcgatg tttctggcgg
    15841 tcgaatggcc gcgattgctc tccacccgcg cgacatctcg atagagcacg gtgcgcggct
    15901 cgtcggtctg gcgatcacgc acgataaact cttcttcctt cgcctgcgag atataacctt
    15961 tcatcttcgc cccgtctttg aacttcaccg tgacgcgcgc acgctcgccg acgccgagcc
    16021 gcgcaacttt aaccttcacc tgctccaacg tctgcgcgtc gcgctccgcc tgcgtcttct
    16081 caacggcaac cgcagcagca acggacggta ccccgaacga cgtttgcagc aacagcgcga
    16141 ccagcgcgaa caccaaatat tttctcttca tttttcattc cctttccgac atttgaaact
    16201 ccaaagctga ccttattgca aaagtgatgc cgcctgaaag agagtgaaaa gagccgccaa
    16261 aacgataggt gacaggtaaa agcaggcgcg aaacgaaaat ttctctgcgc agtaaacttc
    16321 agacagatgg ttaaaagcag taacgagtga caagtaacga gtgacaagtt aaaaccgtaa
    16381 atcttgaacc gtgaatcgtg aatagaagaa gacggttttt tttctattcc gattgatgat
    16441 tcaccgattg acttgtcttc tctcgtcact cgtcactcgt cacttgtcac tcgtcactgc
    16501 ttttccgact tgacagaagc ggcgcacctc gccgatacat acatgaagtt tctcccatgc
    16561 taaagagcgc gccccgcggt cgggcgacat caaaagttaa atttaaaaac tcatgcgatc
    16621 tacagttcac acggaaaagg tatcgcgccg cgccgtcgag ttgcgccgcc acgcgctcgc
    16681 ctgcgcgctg gcgttgacgt tcggcctctg cttcatctct tcgcacgcgt tcgcgcaaaa
    16741 gcggcgcacg ccgacgccgc gacgcacgcc cgcacgcgcg ctgtcgatcc ccgaagagac
    16801 tctgctgcga attgtgcgcg ctgaagacga gagacgtttt gacgcggcag acctcggcgc
    16861 gctgctctcg gatgccaacg cgagcgtgcg cgcacgcgcc gcgctcggcg cgggacgcat
    16921 cggcgacgtg cgcgccgtcg cgccgctcct ctccctgctc cagacagacc gcgacgaacg
    16981 cgtgcgcgag gcggcggcct tcgcgctcgg cgagacggaa gcgggcgcgg cggcagaagg
    17041 gttgctcagg atcatcgaac gcaagacggc tgcgagcgtc gcggaaacgc cgcgcgtgcg
    17101 tgcgcgtgcg atagaagctt tggggaagat cgcggcggcg ttgccgaagg ccgaagagga
    17161 gcgcgccaga gagatcggaa agtctttgct ggtcgcgctt gagtttgaag cgcgcccgtc
    17221 gcagcagccc gcgcagcgag cggcgaatcg agaggtcgcg ttgctcggac tcacggcggt
    17281 cttgcgcgca cgcccggaag acgcggggcg cgtcgtcgcg ctctttttag actcgacgga
    17341 cgcgcgcctg cgcgccgatg ccgcgaacac catcacgcgc ctgcgcgcga agaacgccaa
    17401 cgatcaactg cgcaggctcc tcgcctctga tgcggacgcc gttgtgcgcg ccaacgccgc
    17461 acgcgcgctt ggcgcggcgg aagacaaaac tgctttcgac ttgttgctcg aacgcgccgc
    17521 gaacgacaga gacgaacgcg tgcgcgtcag cgcgattcgc tcgctcggaa cgctcaagga
    17581 tgcgcgcgcc gccgaaccgc tcgtcaaacg cggcgagctt ttgttgagcg cgtatcgtgc
    17641 ggcgaagtct gcgaacgccg ccgcgcgtcc cgccgagttg aacgaactgc tggaacttgt
    17701 cacgggcttg agccgcgtcg tcgcgggcac ggcgaacgag cgcgctctcg gcctgctgcg
    17761 cgcgctccgc gaagccgaac aatggagcac gcccgaagcc gagatcgccg ccgcacgcat
    17821 cgcgcccggt caatacctgc gcgaacgccc cttccaccaa ctcgctttcc gcgcgcccga
    17881 cagtcgcatc tcgtggcaga cctacgccgc catcgcgcag ggcttgggcg agatcgccgc
    17941 cgtgtcgagc gggcgcgcgg gcaacagcgt cgtgtcggtt caggccgatg cgcagttagc
    18001 cctgcgcacg atgcttagtg acacgacgac gcccgcgctc gccgtgcccg acgtgctcac
    18061 ggccatcgcc gccttcaaac cgcccgacgg cgcgctcgtt atgcgcgcgc aactcgccgc
    18121 gccggacgtg atcgtgcgcg ccaccgccgc agggcaacta ggcgaactcg cgcccgatga
    18181 cgaaacgacg agcgcgctcg ccgccgcgct caacacagcc ctgcgcgacg agatgaacga
    18241 tgccgcgctc gccatcctcg acgcgctcgc aaaacagaaa tccgaaccgg ccatcgccgc
    18301 gatcaagacc gcgctcgact cgcccgacta cctgttgcgc cgccgcgccg cagccatttt
    18361 gcaggccgcg caacccgatg cagccaaccc ggacgaaggg cgcatcgaaa cggtcgccac
    18421 gcgcaaccgg cttcccgatt atcgtcgcgc gctctcgcgc cgcgcctcac gcgtcgtcgc
    18481 cacggtcaac acggacaagg gcgcattcca gttggaactg ctgccgaacg aggctccgct
    18541 cacggtggat aatttcgtcg aactcgcgcg acgcggcttc ttcaacaaca tctcttttca
    18601 tcgcgtggtc ccgaacttcg tcgtgcaggg tggcgacccg cgcggcgacg gcaacggtgg
    18661 tcccggttat caaattcgct gcgagatcaa cgaagtgccg tacggtcgcg gcgcggtcgg
    18721 catggctctg tcgggcaaag acacgggcgg ctcgcagtgg ttcatcacgc attcgccgca
    18781 gccgcatctg gacggcggct ataccgtctt cgcacgcgtc gttacaggca tggagacggt
    18841 tgaccgcatc acgcgcggcg accgcatccg cagcatcaac gtcgcggaga cgaagcacaa
    18901 gtgagcaaga cgcgtctgag cgcgccggag taattcgacg cgtgcaccga catcccgcac
    18961 gagacgattg agggtcggca actttatcat ttagacgaat gagccaattt atcaagcaga
    19021 cgacgcgcat caaattcggc ctcttcgtgt cggcgttgct ctgcctcgtc tccacgctcg
    19081 cctccgctca atcgccgcgc gcctccgcat cgtctcccgc ctctcaggcg agcaaacccg
    19141 caccggcgcg cacgcgctac cagatcaatt tcgcgctcga cttcgacgcg cgcaccttta
    19201 gcgcgaccga gcgcgtccgc tgggtgaatc gtgatcaacg ccccgcctcc gtcgtctatt
    19261 ttcatctcta cgcgaacctg cgcagcaata gtaacggcaa caacaacgac gcgaaccgcg
    19321 cccccgcgcc tcccgtgagc ggcgcagagg cgacggcagc cgcaacagca atagcggcga
    19381 cggcggtgga tgaaccgcgc atcgagatca agagcgtccg tcacgccgcg acgcaaaccg
    19441 cgctcgccta cgcgctcgac gagcaggcga cgctgttgcg catcaatcta cgcgaacccg
    19501 tggccgccgg agcgacgacc gagatcgaga tcgcgttcac aggagccgtg cccgaaatag
    19561 acccggacga aacgagcctc ccttcacacg tcatccagca actcggcgca gccctgcgcg
    19621 acacgcgcga gacgcgccgc gcacgcgaca ccaactttcg cgcgcgcggc atcttgatgc
    19681 tcggctcggc ctaccccgtg ctcgccgtgc gcaacggcga cgagtggcag cgcgaagtgc
    19741 gcgcgacggt cggcgacccg gcgcacacgg aagtcgccga ttatgaggtg acgattgatg
    19801 cgcccgccga ggtcgcgctc tacacttcgg gcgaagcgcg ctcccttgcg ggcggcagta
    19861 cggttaaccc agatagcggc agcagcaatg ttaagcccgg cagcggggcg gacgccgtga
    19921 aaagcggcgc gcgtctcttc gccggagaga acctgcgcaa cttcgcgatc atcgcggggc
    19981 ggacgcttgc ctctggcgaa cgcacggtcg gcggcgtgcg cgtgcgttcg gtctggacga
    20041 cggaacacga aaagatcggg cggcgcgtgc tcgatgcggc ggcggaggcc gtgcgcgtct
    20101 tcacggtgcg tttcggcgcg ctccccgtca cgagcgtcac ggtggcggaa gcaccccttg
    20161 tcgccggact cggcagcgcg gagtttgcca acctgagcgt gatcgccagc gcgttctatg
    20221 tcgacttcga ttcgcccgcc atacgcaacc tgcccgaact cgtgcgcgag cagcgcgcct
    20281 cggtcgaaga cagtctggag tttgccaccg cgcacatgct cgcgcatcaa tggtggggga
    20341 tgctcgtcgg caactcgccg gaacgcgccc ccgtgctcga tgaagcgctc gcgcactggt
    20401 ctgcgctcgt ctattaccgc gagacgcacg gccacgttcg cgcgcaagcg gcgcgtgacg
    20461 atcaactgcg cggcgtctat gaaatttatc gcaccttcgg cggcgacgac atgcccgccg
    20521 accgtcccgc gcgcgactat cgcaactcgt ttcaatacgc cgccatcgtc tcggccaaag
    20581 gtgcgctgat gtacgacgcg ctcgtgaaac tgctcggcga ggagcgcgtc ttcacggcct
    20641 tacgccgcta cgcggaggcc aaccgtttcg agatcgccga actcgacgac ctgcgcggag
    20701 ccttcatcgc cgaagcgccc gtcgccgagc gtcgcgccgt cacgcgcacc ttcgaccgct
    20761 ggctctccga gcgtcacggc gacgaagaca tcgccccgcc caacccgcaa ctcgcctccg
    20821 cgctcggcct cacgcccgaa cccgcaggca cacccaatcg caacgccttc tcacgcctcg
    20881 gcagattctt ctggcgacag atgacgcgca ttcgttaacc acgcgccatt gcgcccggct
    20941 aaatcttttt aagttcagat tcgtctcagc gcgtccattc ataaaaattg gaagtgctcg
    21001 ccgtcgcgcg cgcggggtag ttccagttcc gccctcccgc ctcctgatcc gtctgtttga
    21061 acggtcagct tgatgccgcc tgcgtcaaat ggaccggagg cgcgcccgtg cgcttcatgc
    21121 gtttgcggtt ggtgaaggta tttccagaaa atgtgtgcgc ggcacggtgt ttgtttaact
    21181 gtcagtgttc aagcgtgaac cccttcgcgc aatcc
//
LOCUS       DASWNS010000592        13223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_360347, whole genome shotgun sequence.
ACCESSION   DASWNS010000592 DASWNS010000000
VERSION     DASWNS010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13223
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360347"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VGB76_20350"
     CDS             complement(<1..606)
                     /locus_tag="VGB76_20350"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0101286.1"
                     /translation="MEIKKVGVLGCGLMGSGIAQTAATAGFEVVVREVSNELCERGFA
                     GIEKSLAKFAEKGTINADQQTEIRGRLRGTTEFADLADCDIIVEAIIENLDTKRDTYR
                     QLDELCKPETIFASNTSSLSITEMMTATSNERQRRFIGLHFFNPVPIMKLVEVVKTIL
                     TDEAVYETAVDFGKRLGKVPVRASDKTGFIVNRLLVPYMLDS"
     gene            complement(662..2125)
                     /locus_tag="VGB76_20355"
     CDS             complement(662..2125)
                     /locus_tag="VGB76_20355"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY0101287.1"
                     /translation="MSEAEPARDMYAGRPSVCRNCGMLVGAGESACAICHAPVGSTPQ
                     DAASDASQQQPEETVSDIKTRRFRRVLLRRPATFTFVFLVVNVFIFLLMSSAAPGGSQ
                     NPEVLRAYGAKFNWLIDEGEWWRFVTPVFLHIGGLHLLVNMYSLFILGPYVEKLYGSA
                     RFVFFWIATGMAGVLASYLASSAGARGGVLARFLFRGGGDAPSAGASGALFGLIGVLF
                     VFGIKFRHELPEGFKRAFGTGMIPTILINLFIGYLIPFIDNAAHLGGFVAGAVLALFV
                     GYKRPGQRASVAILWHVLQFASLALVVVAFGQVARHMGDARPLPDNTAAAQPNAATID
                     RYVQAMNEGQEAFRAAFNERDTKAANSAIGKIDQVAPFAPRPDQLRLELRQLLVRAHD
                     LALLSEKERTSARVRGELKQLEADFNAWEQRSDEWVQAEGENYGIVLVNKDEEAAPAA
                     SPAPQTSPQAAPETSPQTAPQASPQAPPQSSPAPPER"
     gene            complement(2399..4648)
                     /locus_tag="VGB76_20360"
     CDS             complement(2399..4648)
                     /locus_tag="VGB76_20360"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HEY0101288.1"
                     /translation="MSGKRLILVGVSALLALSFAVREARAQAGDVDESEQKDVEVSIP
                     DRGRVRAPELKNDRGWLNTERPLSLAALRGKVVLLDFWTYGCINCIHIIPDLKRLEAK
                     YPNNLVVIGVHSAKFENEKQSENIRQIILRYEVEHPVVNDADFSIWQSYAVRAWPTQV
                     LIDPAGYVVGKVEGEGNYEVIDGAIAETLAEFRKRGELNEQPLKLALERAKVGDMPLA
                     FPGKILADAKTERLFIADSNHNRIVVAKLDGTLLDTIGTGERGATDGAFERATFHRPQ
                     GLALDADALYVADTENHLIRRVDLKARTVSTIAGTGAQAAGGFGEETGRAPAQSTPLN
                     SPWDLQLAQRTLYIAMAGPHQIWQLDLARGQISVFAGSGREARLDGARLSSAFAQPSG
                     LTTDGSKLYVADSESNIIRAIDLSAPQAQAAPTPEVNEARDPTETDAPDAPEVSPSPE
                     QPDETESATASMQTSMPAPALQVETLAGGDLFEFGDTDGTGDAVRLQHPLGVLYTDGA
                     VFIADTYNHKIKRLDLQTRTVKTFAGTGKPGQADGANASFYEPGGLSLAHGKLYIADT
                     NNHAVRVVSLADGRASTLPLRGLRPPAASVKKLEADAAAMPAPNAEELKLEPQPLLLT
                     RAATASGAANTLVVEVALPAGYHLNELAPHRLQATIERGAEHVAFAGNTRQLTRAAKD
                     LLRLPLRLPLHAYTVGAAELRLQLTLYYCREDNTGACRIKTLIWRAPIRITSDPAAPR
                     EIKLRGEVK"
     gene            4912..5493
                     /locus_tag="VGB76_20365"
     CDS             4912..5493
                     /locus_tag="VGB76_20365"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY0101289.1"
                     /translation="MEESTQDDSTAGGSTRAGRLTGARRKLCVMRSGARLFAVYADEV
                     AATSEDSRPTPLPFAPLPVRGIVSQRGRILTVIDPLPLLPPDAPTLAATPEAPAHTSP
                     QASSLTQPHAPLIVALRGDEQLALSVERIEHDLELFDDEDDHATDTTANASPGSHLLR
                     RTIRHETDSVAILDTAHLFEAAMQGVERRRRRT"
     gene            5585..8119
                     /locus_tag="VGB76_20370"
     CDS             5585..8119
                     /locus_tag="VGB76_20370"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY0101290.1"
                     /translation="MNEQSFGVLEYDKLRALVRRAAQTPVGRARVERLAPLDNLEAVR
                     SALGAVAECVELRRRGGAWSFSELADPAESIARLRIEGATLEPLPLLELARLCDQVGA
                     ARALIQAERDDVPVLWSLVADLPRELYSLAARVTSKILPSGELDDRASPELARIRHDI
                     TRLRSSITRSLENLMRRSDEAIQDQLVTVRNDRFVIPVRSDHRGRVSGVAHGFSSSGA
                     TVFIEPLETIDSNNELQNLRETEEREITRILSTLTDEMRRELPAIERAAEAVAELDFI
                     NAKASMAVRFNCVQPFVGADAALELDAARHPLLEENLRASGGAVVPVSFRLDTTHPVM
                     VISGANAGGKTVVLKTAGLLALMALSGLHVPARAASFPFYQSVLADIGDSQSLAANLS
                     TFTAHVANISRMLEICRSPALVLLDEVGTGTDPEEGSALGVAVVDHFTRACGAHVVAT
                     THYSGLKQYAANESGVINASVEFDEKTLQPTYRLLVGLAGSSSGIEIAGRFGFPQEIV
                     RAASEQVNDASRAATEYLRRIKRESEEAEALRRALEEERAAVAEKYSTLERDADRRER
                     ERQTSFEREMRERLAEFEQRSRELFSKIEDRAERIRVEREAATRAAELRREAQRAAAA
                     SARPNATTGARPVATKETHGEQPGGVRVVRQARAEIESPRVKSGGETFQPSAVEREIV
                     KGDTVRLKTLGKTGVVERVSGADAEIRIGHVRLREKLSNLELMERAPQGKQEQARGAS
                     QLEQMAAAAARTADVRLSRSKDEPAMELNLIGRTTDEAVDAADKFLDSAYLNGLARLR
                     IIHGHGTGALRRAIADFLRSHPHVETFAPAPPDQGGAGATVVELKQ"
     gene            8139..9230
                     /locus_tag="VGB76_20375"
     CDS             8139..9230
                     /locus_tag="VGB76_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101291.1"
                     /translation="MQIFRRRVGRKYLAAMLLSVSFILTLCAPASAQLDQWGYWENGV
                     TEPWWFSSEDFTKDDAVAALARWKNAGSAQQDVTTAEWDGVYFNGSETHGTYLRWSPR
                     GGFIIAHIDKCQARVMGLTYGRVEASPTLLQFFPEFDKAASRTHGHSHAREKSAPTVL
                     RFVPVAWRGQRMLVVESEMSSFGDYVAGRGRYNYNRDFFYLDYVEFFTQRDATADTQP
                     ITTQLTQTEEKTADDATPPVVPPAYVRFLKPPIEGAITAVGKREVRNNYSYQSPDGSE
                     TAYGRVSLTYVTVNAGAARGLKSGMFLRVAEPDGGELVRIIRAGRHASSGIVIRSLDE
                     ADAETFFAEGQTYSRPKVTAGWKLTTAPF"
     gene            9353..9640
                     /locus_tag="VGB76_20380"
     CDS             9353..9640
                     /locus_tag="VGB76_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101292.1"
                     /translation="MTRQQFIEEIKQLSIAERIALIEAITRSLREDLTTGREAASISA
                     AGQAGTSGEGERERKLAAVRRLRGIAKFEGEPPSDEALKEDYVNYLTEKYS"
     gene            9640..10059
                     /locus_tag="VGB76_20385"
     CDS             9640..10059
                     /locus_tag="VGB76_20385"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0101293.1"
                     /translation="MRVLLDSDVVLDFVLERKPFVEAAGELFELIAQGACDRYVSGIT
                     PINVFYLMRKAKGIDKARQAIGDLLIAFQVCPITHTVLKDACALPFADYEDAAQHASA
                     TASSLDAIVTRNLDDYKNATLPVYSPSDFLNHLKAQT"
     gene            10064..11896
                     /gene="dnaG"
                     /locus_tag="VGB76_20390"
     CDS             10064..11896
                     /gene="dnaG"
                     /locus_tag="VGB76_20390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY0101294.1"
                     /translation="MRFPQTFIDDLRRQADIVRVVQDYVSLKKKGANWMACCPFHQEK
                     TPSFSVNPSKEIFYCFGCQKGGSVFNFVMEIERVSFPEAIKIVAEKVNMPLPVMQENE
                     RFESRRKEADEVVELNSWALEWWEGQLAEGNAEAKTAREYIEGRGITDETRKTFRLGY
                     APDSWDGLGSYLKRRGATAAQIERSGLVVKKDAGGFYDRFRGRVIFPVLDAQGRAVAF
                     GGRVMGAGEPKYLNSPETAAYTKGRHLYGLHQTRDEIRRKKFAILVEGYLDLIIPYQF
                     GVQNAVASLGTALTPEQAKLLGRFARRVVVNYDGDRAGVSAAKRAIETLLAEDFDIKV
                     LVLPEGADPDEFIKAAGVEAYNERRGAALPHIQFVLDQAMRNRNLRRPADKAEAVEEV
                     LPFVRAVRNRIQKREYFDMTMDALRIEEPALRRELWQTIAKPNPTAQSGADASAALQD
                     KVVRAESVPLTVAEQRLLELLVHDAELRRILLPKIVPDDYEDLPTARVYRALAEIEAA
                     GAEVDFGTLGERTEGDPVASDLVPLLLMSEPARDEGEATDEVLAEAESCLLTLRLMRV
                     ERRLKDLALEIAAAERAGDNERRDALVMENLTWTRRRSALVQRS"
     gene            11956..>13223
                     /locus_tag="VGB76_20395"
     CDS             11956..>13223
                     /locus_tag="VGB76_20395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0101295.1"
                     /translation="MEEKDQDDVVQRLLELGSDKKYLTFDDLNRELPDSLVSPDDIED
                     VLQKLDGANIIVADSDERLIEQAAQSSVASPEDEEPADEEEVNLDLSAGMLDKTNDPV
                     RLYLREMGVVPLLTREGEVTIAKRIERGQIRTRKAISRSPIAVSELLRIGDDLEALKI
                     SIRDVVTFAEQAELAGVEDKAEEYYQWTAEGINNIRRHFKAGLKEWERLRAEQKLARG
                     KEAKKLLRLKRRVARTRLEIAQEIGQLNLTERARQRMIGAIRAVAREMRAAERDIDKY
                     TERLGKRRLKPEEERDYKRHIASARRKLQQIEAETLISPVEIKRSLQTIIASEHETGQ
                     AKRELIEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEWRRGYKFSTYAT
                     WWIRQAITRAIADQARTIRIPVHMIETINKL"
BASE COUNT         2570 a         4081 c         4219 g         2353 t
ORIGIN      
        1 ggaatcgagc atgtaaggca cgagcaggcg attgacgatg aagcccgtct tgtcgctggc
       61 gcgcacggga actttgccga gtcgcttgcc gaaatcgacc gccgtttcgt agaccgcttc
      121 gtccgtcagg atcgtcttaa cgacttccac gagtttcatg atcggcacgg ggttgaagaa
      181 gtgcaggccg atgaaacgac gctggcgttc gttggaagtg gccgtcatca tctccgtgat
      241 cgagagcgag gaggtgttgg aggcgaagat cgtttcgggc ttgcagagtt cgtcgagttg
      301 tctgtaggtg tcgcgcttgg tgtcgagatt ttcgatgatg gcttcgacga tgatgtcgca
      361 atcggcgagg tctgcgaact ccgtggttcc gcgcaggcga ccgcgaatct ccgtctgctg
      421 gtcagcgttg atcgttccct tctcggcaaa cttcgcgagc gacttttcga tccccgcgaa
      481 gccgcgctcg cagagttcgt ttgagacttc gcgcaccacc acttcgaacc cggcggtggc
      541 ggcggtctgc gcgatgccgg aacccatcag gccgcagccg agcacaccta cttttttgat
      601 ttccatttcc gtttcaattc tcctgtcttc gattttgctt gcatgttaaa aatcctgttt
      661 ctcacctctc cggcggcgca ggcgaactct gcggcggagc ttgtggcgat gcctgcggcg
      721 cggtctgcgg cgaagtttcc ggcgcggcct ggggcgaagt ctgcggcgca ggcgacgcgg
      781 cgggcgcggc ctcttcatct ttgttcacga ggacgatgcc gtagttttcg ccctcggcct
      841 gcacccactc gtctgaacgc tgctcccacg cgttgaagtc cgcttccagt tgcttgagtt
      901 ctccgcgcac gcgtgcgctg gttcgttcct tttcgctcag gagcgcaagg tcgtgtgcgc
      961 gcacgagcag ttgtctgagt tcaagccgaa gttggtcggg tcgcggcgcg aagggcgcga
     1021 cctgatcgat ctttccgatg gcactgttgg ctgccttcgt gtcgcgctcg ttgaaggccg
     1081 cgcggaaggc ttcctgtccc tcgttcatgg cctgcacgta gcgatcaatc gtggccgcgt
     1141 ttggctgcgc cgccgccgtg ttgtcgggga gcgggcgcgc gtcgcccatg tggcgcgcga
     1201 cctgcccgaa ggcgacgacg acgagcgcga gcgaggcaaa ctgtaggacg tgccacagga
     1261 tggcgacgct cgcgcgctgg ccgggtcgtt tgtaaccgac gaagagcgcg agcaccgcgc
     1321 ccgcgacgaa gccgccgagg tgcgccgcgt tgtcgatgaa aggaatcaag tagccgatga
     1381 acaggttgat gaggatcgtc gggatcatgc ccgtgccgaa cgcgcgtttg aaaccttcgg
     1441 gcagttcgtg cctgaacttg atgccgaaaa cgaagagcac gccgatcaga ccgaagagcg
     1501 cgcccgatgc gcccgccgaa ggagcatcgc cgccgccgcg aaagaggaaa cgcgccagca
     1561 cgccgccgcg cgcccccgca ctcgaagcca gataactcgc cagcactccg gccatgcccg
     1621 tcgcgatcca gaagaagacg aagcgcgccg agccgtacaa cttctccacg taagggccga
     1681 ggataaagag gctgtacatg ttgaccagca ggtgaagccc gccgatgtgc aaaaagaccg
     1741 gcgtgacgaa tcgccaccac tcgccctcgt caataagcca attgaacttc gcgccgtaag
     1801 ccctgagcac ctctggattc tgcgagcctc cgggcgcggc agaactcatc aacaggaaaa
     1861 tgaagacatt gacgacgaga aagacgaagg tgaaggtggc gggacgcctc aggagtacgc
     1921 gccggaaacg acgcgtcttg atgtcggaga cagtctcttc cggttgttgc tgcgacgcat
     1981 cggacgccgc atcctgtggc gtgctgccca caggcgcgtg gcagatcgcg cacgcgcttt
     2041 cgcccgcgcc gacgagcatg ccgcaattcc ggcacacgct cggacgcccc gcatacatgt
     2101 ctctcgctgg ctctgcctct gacaaaagtt cgtccctcga cgagcgcgcc cgtcaaactc
     2161 tctcgcacgc ccccgcctca aacgcagtac acacgcccgc cgtcaccctg cccgactcac
     2221 acgagccgcc gcgcgccctg ctgaatatca cgaattattc attcgaccga aagtttacac
     2281 cgaagcacac gggacggaaa gaagggatga gggcggaggg atgagggatg aatcagaaac
     2341 tactcaactc gcttgagtgc tttctctgat tcatccctca tccctcatcc ttcatccctt
     2401 atttcacttc tccgcgcagt ttgatctcgc gcggggcggc ggggtcggag gtgatgcgga
     2461 tgggtgcgcg ccagatgagc gtcttgatgc ggcacgcgcc ggtgttgtct tcgcggcaat
     2521 agtagagcgt gagttggagg cgcagttccg ccgcgccgac cgtgtatgcg tgcaagggta
     2581 gacgcagggg gaggcgcagc aaatctttgg cggcgcgcgt gagttggcgc gtgttgcctg
     2641 caaacgcgac gtgttccgcg ccgcgttcga ttgtcgcttg cagacgatgc ggcgcgagtt
     2701 cgttcaaatg ataaccggcg ggcaaggcca cctcgacgac gagcgtgttc gcggctccgc
     2761 ttgccgtcgc tgcgcgcgtg agaagaagag gctgcggctc aagtttcaac tcctcagcgt
     2821 tgggcgcggg catcgcggcg gcgtctgctt ccaacttctt cacgctcgcg gcgggcgggc
     2881 gcagaccgcg cagggggagc gtggaagcgc gcccgtcggc gagggaaacg acgcgcacgg
     2941 catggttgtt cgtatccgcg atgtagagtt tgccgtgcgc gagggagagg ccgccgggtt
     3001 cgtagaaaga ggcgtttgcg ccgtccgcct gtccgggctt gcccgtgccc gcgaaagttt
     3061 tcacggtgcg ggtttgcagg tcgagccgct tgatcttgtg attgtaagtg tccgcgatga
     3121 agaccgcgcc atccgtgtag agcacgccga gcgggtgctg caagcgcacc gcgtcgcccg
     3181 tgccgtccgt gtcgccaaac tcgaagaggt cgccgcccgc gagcgtctcc acttgcagcg
     3241 caggggcggg catcgaagtt tgcatcgaag cagtcgctga ctctgtctcg tccggctgtt
     3301 ccggcgaagg agatacttcg ggagcgtccg gcgcgtccgt ctccgtaggg tcgcgcgctt
     3361 cgttcacttc gggcgtcggc gcagcctgtg cctgcggcgc agacaggtcg atggcgcgaa
     3421 tgatgttcga ttcgctgtcg gcgacgtaga gtttgcttcc gtcggtggtg agtccggagg
     3481 gctgcgcgaa ggcggaggag aggcgcgcgc cgtcgagccg ggcttcgcgc cctgatccgg
     3541 cgaagacgga aatttgaccg cgcgccagat cgagttgcca gatttgatga ggtccggcca
     3601 tcgcgatgta gagcgtgcgc tgcgcgagtt gcaagtccca gggtgaattg aggggggtgc
     3661 tttgcgcggg cgcacgcccc gtctcttcgc cgaaccctcc ggcggcctgc gcgcccgtgc
     3721 ccgcgatggt cgagacggtg cgcgccttca ggtcaacgcg acgaatcagg tggttctccg
     3781 tatcggcgac gtagagcgcg tcggcgtcga gcgcgagtcc ctgcgggcgg tgaaacgtag
     3841 cgcgttcgaa tgcgccgtcc gtcgccccgc gctcgcccgt gccgatggtg tcgaggagcg
     3901 tgccgtcgag tttggcgacg acgatgcggt tgtgattcga gtcggcgatg aagaggcgtt
     3961 cagttttggc gtcggccaga atcttaccgg gaaaggcgag cggcatgtcg cccaccttcg
     4021 cgcgttcgag ggcgagtttg agcggttgct cgttcaactc gccgcgcttg cgaaactccg
     4081 cgagcgtctc cgcgattgcc ccgtcaatca cttcatagtt gccttcgcct tcaaccttgc
     4141 cgacgacgta tccggcggga tcgatcaaca cctgcgtcgg ccacgcgcgc acggcgtagg
     4201 actgccagat cgagaagtcc gcatcgttga cgaccgggtg ctcgacctcg tagcgcagaa
     4261 tgatctggcg tatgttctcg ctctgttttt cgttctcgaa cttggcggaa tggacgccga
     4321 tgacgacgag gttgttcggg tatttcgctt cgagccgttt gagatcgggg atgatatgga
     4381 tgcagttgat gcaaccgtaa gtccagaaat ccagcagcac gactttgccg cgcagcgcgg
     4441 cgagcgagag cggtcgctcc gtgttgagcc agccgcgatc attctttagt tcgggcgcac
     4501 gcacgcgccc gcgatccggt atggacactt cgacatcctt ttgctcagac tcatccacgt
     4561 cgcccgcctg cgcccgcgcc tcgcgcaccg caaacgaaag cgcgagcaga gccgacacgc
     4621 cgacaaggat caaacgctta cctgacatca aaactcccca agacttcgac accccctcca
     4681 aaatttcccg cacttgaagc tgcaaacccg gtgaaactgc aaacatcgtg aaactgcaaa
     4741 tatggtgacg cgcacaatat acgcgcccgg gcgagcgggc agattcaccc ggcgcgacgg
     4801 gcgcaaaagt ctgccccgaa gtctcccacg cgcgcacgag tcccggttcg cgacgctcgc
     4861 actcttcgct tctcgcaaag ttttaccgtt cagtttacca tgttgcctga catggaagag
     4921 tcgacgcaag acgacagcac ggcgggaggt tcgacccgcg cgggacgatt gacgggcgcg
     4981 cggcgcaagc tctgcgtgat gcgttccggc gcgcgcctct tcgccgtcta tgcggatgaa
     5041 gttgcagcga cgagcgaaga ttcaaggccg acgccgcttc ccttcgcgcc cctgcctgtg
     5101 cgcggcatcg tcagccagcg cgggcgcatt ctcaccgtga tcgatccgct cccgctctta
     5161 ccgcccgatg cgccgacgct tgcggcaact cccgaagccc ccgctcacac gtcgccgcag
     5221 gcgtcctctc taacgcagcc gcacgcgcct ctcatcgtcg cgctccgggg cgacgaacaa
     5281 ctcgcgctct cggtcgaacg catcgaacac gacctcgaac ttttcgacga cgaagacgac
     5341 catgcaacgg acacgaccgc aaacgcctcg cccggctcac atctcctgcg tcgcaccata
     5401 cgccacgaga cggactccgt cgccatcctc gacacggcgc atctcttcga ggccgccatg
     5461 caaggagtcg aacgccgtcg ccgccggaca tgataaaaag tgacaagcga cgagtggcaa
     5521 gtgacaggaa aagctgcttt aatcttgtca cttgtcactc gtcgcttgtc acagggtttc
     5581 gcttatgaac gaacaatctt tcggggtgct ggaatacgac aaattgcgcg cgctggtgcg
     5641 gcgcgcggcg cagacgccgg tggggcgtgc gcgtgtggag agactcgcgc cgctcgataa
     5701 tctggaagcg gtcagaagcg cgctcggtgc ggtggccgag tgcgtcgaac tgcgcaggcg
     5761 gggcggggct tggtcgttct cggagttggc cgacccggct gaatcaatcg cgcgcctgcg
     5821 catcgaaggg gcgacgctcg aaccgctgcc gctcctcgaa ctcgcgcgcc tctgcgatca
     5881 ggtcggcgcg gcgcgcgcgc tgattcaggc cgagcgcgac gacgtgcccg tcctgtggtc
     5941 gctcgtcgcg gacttgccgc gcgagttgta cagtctggcg gcgcgcgtca cgtcgaaaat
     6001 cctgccgagc ggcgaactcg acgaccgcgc cagccccgaa ctcgcgcgca tccgccacga
     6061 catcacacgc ctgcgctcct ccatcacgcg ctcgctcgaa aacctgatgc gccgctccga
     6121 cgaagcgatt caggatcaac tcgtcaccgt gcgcaacgac cgcttcgtca tcccggtgcg
     6181 ctcggatcat cgcgggcgcg tctccggcgt cgcgcacggt ttctcttcgt ccggcgcgac
     6241 cgtcttcatc gaacccttag agacgattga cagcaacaac gaactgcaaa acttgcgcga
     6301 gactgaagag cgcgagatca cgcgcatcct ctcgacgctc acggacgaga tgcgccgcga
     6361 actgcccgcc atcgaacgcg cggctgaggc cgtggccgaa ctcgacttca tcaacgcgaa
     6421 ggcttcgatg gccgtgcgct tcaactgcgt gcagccgttc gtcggcgcgg acgccgcgct
     6481 cgaactcgac gccgcgcgcc acccgttgct cgaagagaac ctgcgcgcct ccggcggcgc
     6541 ggtcgtgccc gtctcgttcc ggctcgacac gacgcatccc gtgatggtca tctccggcgc
     6601 gaacgcgggc ggcaaaaccg tcgtcttgaa gacagccggg ctgctcgcgc tgatggctct
     6661 ctccggcctc cacgttccgg cgcgcgcggc cagttttccg ttctatcaat ccgtgctcgc
     6721 agacatcggc gacagccaat cgctcgccgc caatctttcg accttcaccg cgcacgtcgc
     6781 caacatcagc cgcatgctcg aaatctgccg ctcgcccgcg ctcgtgctgc tcgatgaagt
     6841 cggcacgggc acagacccgg aagaaggctc cgcgctcggc gtcgccgtcg tggatcattt
     6901 cacgcgcgcg tgcggcgcgc acgtcgtcgc cacgacgcac tacagcgggt tgaaacagta
     6961 tgcggcgaac gagtcgggcg tgatcaacgc gagcgtcgag tttgacgaaa agactttgca
     7021 gccgacctat cgcctgctcg tcggcctcgc cgggtcgtcc tccggcatag agatcgcggg
     7081 gcgtttcggt ttcccgcaag agatcgtccg cgccgcgtcc gaacaggtca acgatgcttc
     7141 gcgcgcggcg accgaatatc tgcgtcgcat caagcgcgag tcggaagagg cggaggcgtt
     7201 gcggcgtgcg ctggaagagg aacgcgcggc ggtcgccgaa aagtattcca cgctcgaacg
     7261 cgacgcagac cgccgcgagc gcgaacgtca aacatctttc gaacgcgaga tgcgcgaacg
     7321 cctcgccgag tttgaacaac gctcgcgcga actcttctcc aagatcgaag accgcgccga
     7381 gcgcatccgc gtggaacgcg aggccgcgac gcgcgccgcc gaactgcgac gcgaagccca
     7441 gcgcgccgcc gctgccagcg cacgtcccaa cgcgacgacg ggcgcgcggc ctgtggcgac
     7501 gaaggagacg cacggggaac agcctggcgg cgtgcgcgtc gtgcggcagg cgcgtgcgga
     7561 aatagagtcg ccgcgcgtga agtcgggcgg cgagacgttt cagccgtcgg ctgtcgaacg
     7621 cgagatcgtc aaaggcgaca cggtgcgtct gaagacgctc ggcaaaacgg gcgtcgtcga
     7681 aagagtgtcg ggcgcggatg cagaaattcg catcggccac gtgcgcctgc gcgagaagct
     7741 cagtaatctc gaattgatgg aacgcgcgcc gcagggcaaa caggagcagg cgcgcggcgc
     7801 atcacaactc gaacagatgg ccgccgctgc cgcgcgcacc gcagacgtgc gcctcagccg
     7861 ttcgaaagac gaacccgcga tggaactcaa cctcatcgga cgaaccacag acgaagccgt
     7921 tgacgccgcc gacaagtttc tcgattccgc ttatctgaac ggcctcgcgc gcctgcgcat
     7981 cattcacggc cacggcacgg gcgcgctccg ccgcgccatc gcggacttcc tgcgctctca
     8041 cccgcacgtc gaaaccttcg cgccagcccc gcccgatcaa ggcggcgcgg gcgcgaccgt
     8101 tgtagaattg aagcagtaga ctccagaggg gcagtgtaat gcagatcttt cgacggcgcg
     8161 tggggcgaaa atatctggca gcgatgctgt tgtcagtctc tttcatcttg acgctctgcg
     8221 ccccggcctc tgcgcagtta gaccaatggg gctactggga gaacggcgtc acggaaccgt
     8281 ggtggttctc ctcggaagac ttcacgaagg atgacgcggt cgcggcattg gcgcgctgga
     8341 aaaatgccgg gagcgcacaa caggacgtga cgacggcgga gtgggacggg gtctacttta
     8401 acggcagcga aacccacggg acgtacttgc gctggtcgcc gcgcggcggt ttcatcatcg
     8461 ctcatataga taagtgtcag gcgcgtgtga tgggcttgac ttacgggcgg gtcgaggctt
     8521 cgccgacgct cttgcagttc ttccccgaat tcgacaaggc ggcctcccgc acacacggcc
     8581 actcgcacgc gcgcgagaag agcgcgccga cggtgctccg gttcgtgccg gtcgcgtggc
     8641 gcggccagcg gatgctcgtc gtcgagtccg agatgagcag cttcggcgac tatgtcgcgg
     8701 ggcggggcag atacaattat aaccgcgact tcttctactt agattacgtc gagttcttca
     8761 cgcaacgcga cgcgacagcg gatactcaac cgatcactac acagttaact caaaccgaag
     8821 agaaaacagc cgacgatgcg accccgcccg tcgtgccccc ggcctacgtg cgttttttga
     8881 agccgccgat tgaaggggca atcaccgccg tcggcaagag agaggtgaga aacaactaca
     8941 gctatcaaag cccagacggg tcggagacgg cttacgggcg cgtttcgctc acttacgtga
     9001 ccgtcaacgc gggcgcggct cgcggtttga agagcggcat gttcctccgc gtggcggaac
     9061 cggacggggg agaactggtc aggatcatcc gtgccggcag acacgcttcg agcggcatcg
     9121 tgatcaggag tttggacgag gcggacgccg aaacattttt cgccgaaggg cagacctatt
     9181 cccgtccgaa ggtaaccgcc gggtggaagt taaccaccgc gccgttctga acttctcacc
     9241 caccgctatt gctatgcgtt tcccgcagac attcattgac gacctcagac ggcaggccga
     9301 catcgtgcgc gtgatatggg attgcgttag aattggctga cgggagacag tgatgacacg
     9361 acagcaattc atcgaagaga tcaaacaact atccatcgcc gagcgcatcg ctttgattga
     9421 agcgatcaca cgcagtctgc gtgaagacct gacgaccgga cgggaggcgg cttcaatttc
     9481 tgcggcaggt caagccggga catccggcga aggcgagcgc gaacgtaagc tcgcggctgt
     9541 gcggcggttg cgcggcatcg ccaaattcga gggggagccg ccttcggatg aagcgctgaa
     9601 agaggattat gtcaattacc tgacggagaa atactcctga tgcgtgtcct gctcgactcc
     9661 gacgtggtgc tcgatttcgt gttggagcga aagcccttcg tcgaagccgc cggggagttg
     9721 tttgaactta tcgcgcaagg cgcgtgcgac aggtacgttt ccggcatcac cccaattaac
     9781 gttttctatc tgatgcgtaa agccaaaggc attgacaagg ccagacaggc gattggcgac
     9841 ctgctcattg cctttcaggt ttgccctatc actcacacgg ttctcaaaga cgcctgcgcc
     9901 ctgcccttcg ccgactacga agatgcggcg caacacgcga gcgcaacggc aagcagcctc
     9961 gacgcgattg tcacgcgcaa tcttgacgat tataagaatg cgaccttgcc ggtctattcc
    10021 ccgtcggatt ttctcaatca cctgaaagca caaacataac gccgtgcgtt tcccgcagac
    10081 attcattgac gacttgagac ggcaggccga catcgtgcgc gtggtgcagg attacgtctc
    10141 gttgaagaag aagggcgcga actggatggc gtgctgcccg tttcatcagg agaagacgcc
    10201 ttcgttttcg gtcaacccgt cgaaggagat cttctattgt ttcgggtgcc agaagggcgg
    10261 gagcgtcttt aatttcgtga tggagatcga gcgcgtgtcg tttcccgaag cgatcaagat
    10321 cgtggcggag aaggtgaaca tgccgctgcc cgtgatgcag gagaacgaac gctttgaatc
    10381 gcggcgcaag gaagcggacg aagtggttga actcaattcg tgggcgctcg aatggtggga
    10441 agggcaactc gcagaaggca acgccgaagc gaagacggcg cgcgagtaca tcgaagggcg
    10501 cggcatcacc gacgagacgc gcaagacttt tcgcctcggc tacgcgcccg acagttggga
    10561 cggcctcggc tcctacctga aacgcagagg ggcgaccgca gcgcagatcg agcggagcgg
    10621 tctggttgtc aagaaagatg cgggcggatt ttatgatcgc tttcgcgggc gcgtgatctt
    10681 tccggtgctc gatgcgcagg ggcgcgcggt cgcgtttggc ggacgcgtga tgggcgcggg
    10741 cgagccgaag tatctcaact cgccggagac tgcggcctac acgaaggggc gacacctcta
    10801 cggtttgcac cagacgcgcg acgagatcag gcgcaagaag tttgccattt tggtcgaggg
    10861 ttatctcgat ctgatcatcc cttaccagtt cggcgtgcag aacgcggtgg cgagtctcgg
    10921 cacggctctg acgccggaac aggcgaagct tctggggcgg tttgcgcgtc gcgtggtggt
    10981 caattatgat ggtgacaggg ctggcgtctc ggctgccaag cgcgcgattg aaactctcct
    11041 cgcagaagat tttgacatca aagtgctggt gcttcccgaa ggcgcagacc ccgacgagtt
    11101 catcaaggcg gcgggcgtcg aagcgtataa tgagcgacgc ggcgcggcgc tcccacacat
    11161 acaattcgtg ctcgatcaag cgatgcggaa tcgcaaccta cgtcgcccgg cggataaggc
    11221 cgaggccgtc gaagaggtgc tcccgttcgt tcgcgcggtg cgtaaccgga ttcagaagcg
    11281 cgaatatttc gacatgacga tggacgcttt gcgcatcgaa gaacccgcgc tccggcgcga
    11341 actctggcag acgattgcga agccgaaccc gacggcgcag agcggcgcgg acgcttccgc
    11401 agcgttgcag gataaggtgg tgcgcgccga gagcgtgccg ctcaccgtgg ccgaacaacg
    11461 cctgctcgaa ctgctcgtgc acgacgcgga actcaggcgc atcctgctgc cgaagatcgt
    11521 gccggacgat tacgaagacc tgccgaccgc ccgcgtctat cgcgcgctcg cggagatcga
    11581 agcggcgggc gcggaagttg acttcggcac tctcggcgaa cgcacggaag gcgaccccgt
    11641 ggcctccgat ctcgtgccgc tcctcctgat gagcgaaccg gcgcgcgacg aaggcgaagc
    11701 gaccgacgaa gtgctcgccg aagccgaaag ttgcctgctc acgctcaggc tgatgcgcgt
    11761 ggagcggcgt ctgaaagact tggcgttgga gatcgccgcc gccgagcgcg cgggcgacaa
    11821 cgagcgacgt gacgcgctcg tgatggagaa cctgacgtgg acgagacgcc ggagcgcgct
    11881 cgttcaacgc tcgtaagaaa gcggtgcggg gagtaactct tttgaatcca tcaggggtgt
    11941 agaaaattgg ttagcatgga agagaaagat caggacgacg tggtgcaacg cctgttggag
    12001 ttgggcagcg acaagaagta cttgacgttt gacgacctca atcgcgagtt gcccgacagc
    12061 ctcgtctcgc ccgacgacat cgaggatgtc ctgcaaaagt tggacggcgc aaacatcatc
    12121 gtcgccgatt ccgacgaacg cctcatcgaa caggccgcac agtcgtccgt cgcctcgccc
    12181 gaagacgaag aaccggcgga cgaggaagaa gtgaatctcg acctgtcggc agggatgctc
    12241 gacaagacga acgatcccgt gcgtctctac ctgcgcgaga tgggcgtcgt cccgctcctc
    12301 acgcgcgagg gcgaagtcac catcgccaaa cgcatcgagc gcgggcagat caggactcgc
    12361 aaagccatct cgcgttcgcc catcgccgtc agcgaactcc tgcgcatcgg cgacgatctg
    12421 gaagcgttga agatcagcat ccgcgatgtc gtgacctttg ccgagcaggc cgagttggcg
    12481 ggcgtggaag acaaggccga agagtattac cagtggacgg ctgaaggcat caacaacatc
    12541 cgccgccact tcaaagccgg actcaaagag tgggaacgcc tgcgcgccga acagaagctc
    12601 gcgcgcggca aggaggcgaa gaaacttctg cgcctgaaac gcagggtcgc gcgcacgcgc
    12661 cttgagatcg cgcaggagat cggtcaactg aacttaaccg agcgcgcacg ccagcgcatg
    12721 atcggggcga tccgcgccgt cgccagggag atgcgcgccg ccgaacgcga catagacaag
    12781 tacacggagc ggctcggcaa acgacgcctc aagccggaag aggagcgcga ctacaagcgg
    12841 cacatcgcct ccgccagacg caagctccag cagatcgaag cggagacgct catctcgccc
    12901 gtcgagatca agcgttcgct gcaaacgatc atcgcgagcg aacacgagac gggacaggcc
    12961 aagcgcgaac tgatcgaagc gaacctgcgt ctcgtcgtct cgattgcgaa gaagtacacg
    13021 aatcgcggcc tccagtttct cgacctcatt caggagggca acatcggctt gatgaaggcc
    13081 gtggataaat tcgagtggcg gcgcggctac aagttctcga cctacgcgac gtggtggatt
    13141 cgacaggcga tcacgcgtgc gattgccgat caggcgcgca cgattcgcat ccccgtccac
    13201 atgatcgaga cgatcaacaa act
//
LOCUS       DASWNS010000593         6452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_307551, whole genome shotgun sequence.
ACCESSION   DASWNS010000593 DASWNS010000000
VERSION     DASWNS010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307551"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /locus_tag="VGB76_20400"
     CDS             complement(<1..2351)
                     /locus_tag="VGB76_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101296.1"
                     /translation="MSQPTYLTSGSRIAVVGAGPAGSFFAIELLRRARSLGLSLQVCI
                     YDGKNFARKGAPGCNMCAGAVGHNLIEEIEHATTNIPAEVIHYELEGYAVHYQDASAR
                     LENVPGRKIYGVYRGAGPVSTEHPDGARSFDQFLLDQALKLGAQHIAQNVHDIDVPAT
                     LAERPAIRTHEGGREEYDLVVGAFGVNSQLGARLIYGYQPPPTWKTCVIEARVSEEFQ
                     RERLGKLIHFFVLKQADVRYVALIPKGEHVTVTAIGENVDVVSTERLLRESYMRDYLP
                     PDWKLDCHCHPRVAVGAARLPYSDRFCIVGDASDSRYLKNGVESALVTARLAADCVVN
                     HGITARDFEEHFWRRCGQVFGRDNLYGRALFEIYDRIFPHPLIAPVYFAVLQAERKAR
                     TAQRQPDNAAPPAPPPQRGALLSDALLAAFTGDQPYKRILLRALHPRLAARMASHASM
                     LFFDHVRTKVTRSEPLRVRGLQLRDESRVVVIGGGPGGASCAVRLLQEAKKRGLNLRV
                     YIYEPKRFPRQHNPCMGVLVPPTAELLREHLRLKLPAALIKRTIKGFRLHSDHNDIEL
                     RAPLREEPALMVSRSEFDQLLLERAEEAGARVVRDRVTGLEFYPDNLEHPVRVYSEGA
                     SRSADMIVAACGLDDGLLSVLEQASPRAHAYRRPRETMHSIIIRLPCDERYIERRLHG
                     EIHAFLLRQLPRIEFGAITPKATHVLVNIMGRRVTSIDMEDFLLHPKVRALLPRFDLQ
                     KLDFYEGRAPVRHARNVYWDRFVAVGDATGWLRPFKGAGITTA"
     gene            2689..3195
                     /locus_tag="VGB76_20405"
     CDS             2689..3195
                     /locus_tag="VGB76_20405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY0101297.1"
                     /translation="MIIVRAETSKDIEAVRRVNQLAFGRAEEANLVDALRLAAHPYIS
                     LVAETGGRVAGHIFFSPVTIETEVRAPAIFGLAPMAVLPEHQNQGIGSRLVREGLNEC
                     RRISVGAVVVLGHPAYYPRFGFVPASSKGLRCEYPVPDEAFMVTELTPGELPSQGLVK
                     YHPEFGNV"
     gene            3256..3579
                     /locus_tag="VGB76_20410"
     CDS             3256..3579
                     /locus_tag="VGB76_20410"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0101298.1"
                     /translation="MFVALYRWKLREGEEQRFREGWRRRTEEIYRKCGSFGSRLHRAE
                     DGTWVAYAQWPDRRHWEAARQVGVTDTQAPAMMKESIEVSYPEMLLEVADDLFKPRGC
                     LETGE"
     gene            3707..4063
                     /locus_tag="VGB76_20415"
     CDS             3707..4063
                     /locus_tag="VGB76_20415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101299.1"
                     /translation="MALAILNILVALFLGYGAVEEFWVRGVRGGELQPLVVGLVGASV
                     SVLLALAGVGLWRLWPNARGLAVAAAVLSILFHVYAAFPPHRNVGILVLLVGAGYGLI
                     LLCITLTQATRKAQAA"
     gene            complement(4189..5742)
                     /locus_tag="VGB76_20420"
     CDS             complement(4189..5742)
                     /locus_tag="VGB76_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY0101300.1"
                     /translation="MSEEKSGGTKPGVTGGAKEQPELTNRQGHPVYDNQNTRTVGERG
                     PTVLENYQFLEKITHFDRERIPERVVHARGAGAHGYFEAYGRIGSEPASRYTRAKLFQ
                     EKGKRTPVFVRFSTVIHGGHSPENLRDPRGFAVKFYTEDGNWDLVGNNLKVFFIRDPL
                     KFPDVVHAFKPDPVTNRQDPNRIFDFISLTPESMHMISWLFSPRGIPANYREQEGAGV
                     NTYKMVNSEGLGVLVKYHWIPKQGVRSLTQAEADAIGDFNHATKDLFDVIERGDFPEW
                     ELRVQIMSDDEHPELDFDPLDDTKIWPEVQFPMLPVGRMVLDRNPENYFAEVEQVAFG
                     TGVLVDGLDFSDDKMLQGRTFSYSDTQRYRVGTNYLQLPINRPKKHVATNQRDGQMAY
                     FVDGNEAGENPHVNYEPSSRGGLQEAQRAGKEHAPYVQGAVVRQKISRTNDYKQAGER
                     YRTMEDWERDDLVNNLASALKQCNPDIQERMISLLTKCDEDYGRRVAEGIGGGGSVET
                     SPQQATAAD"
     gene            complement(5806..6243)
                     /locus_tag="VGB76_20425"
     CDS             complement(5806..6243)
                     /locus_tag="VGB76_20425"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0101301.1"
                     /translation="MTAKKKISEREALRQTLGEKGVHLTSQRAAVFDYLSGVEHHPTA
                     EEIYLAIKPKLPRISLATVYKNLEALIKCEVVSKLTYGDASARYDIRTDHHYHTRCLE
                     CGRVWDLDAVKGSAWLERVKPQAGFEVHDYRLEILGRCRDCRR"
BASE COUNT         1172 a         1835 c         2110 g         1335 t
ORIGIN      
        1 atggcagtgg tgatccctgc gcctttgaac ggtcgcagcc aacccgtcgc atcccccacg
       61 gcgacgaaac gatcccagta gacattgcgc gcgtggcgca cgggcgcacg cccttcgtag
      121 aagtcgagtt tctgcaaatc gaagcgcggc agcagagcgc gcactttcgg gtgcaggaga
      181 aaatcttcca tatcaatcga agtgacgcgc cgccccatga tgttgacgag cacgtgcgtc
      241 gccttcggcg tgatcgcgcc gaactcgatg cgcggaagtt gacgcaggag gaaggcgtgt
      301 atctcgccgt gcaggcgacg ctcgatgtag cgttcgtcgc agggcagacg gatgatgatc
      361 gagtgcatcg tctcgcgcgg gcggcggtaa gcgtgcgcgc gcgggctggc ctgttcgagc
      421 acggagagca acccgtcgtc cagaccgcag gcggcgacga tcatgtccgc cgagcgcgac
      481 gcgccttcgg aatagacgcg cacgggatgt tcgagattgt ccgggtaaaa ttcgagtccg
      541 gtgacgcggt cgcgcacgac ccgcgccccg gcctcttcgg cgcgttccag caatagttga
      601 tcaaactcgg agcgcgacac catgagcgcg ggttcttccc tgagcggcgc gcgcagttcg
      661 atgtcgttgt gatcgctgtg gaggcgaaaa cctttgatcg tgcgtttgat gagcgcggcg
      721 ggaagtttga gtcgcaaatg ctcgcgcagg agttcggcgg tgggcggcac gagcacgccc
      781 atgcaggggt tgtgctggcg cgggaaacgc ttcggctcgt agatgtagac gcgcaggttc
      841 aacccgcgct ttttcgcctc ctgtaacagg cggaccgcgc acgacgcgcc gccgggtcca
      901 ccgccgatga cgaccacgcg cgactcgtcg cgcagttgca ggccacgcac gcgcagaggc
      961 tcgctccgcg tcactttcgt ccgcacatga tcgaagaaga gcatgctggc atggctcgcc
     1021 atgcgcgcgg cgagccgtgg atggagggcg cgcaagagta tgcgcttgta gggttggtcg
     1081 cccgtgaagg cggcgagcag cgcgtccgag agaagcgcgc cccgctgcgg tggcggcgcg
     1141 ggcggcgcgg cgttatcggg ctgccgctgc gcagttctgg ctttgcgttc ggcttgcagc
     1201 acggcgaaat agacgggagc gatcaggggg tgcgggaaga tgcggtcgta gatctcgaag
     1261 agcgcgcgcc cgtagaggtt gtcgcgcccg aagacctgcc cgcaacgacg ccagaagtgc
     1321 tcttcaaaat cgcgcgccgt gatgccgtga ttgacgacgc aatccgctgc gagtctggcc
     1381 gtaacgagcg cgctctcgac gccgttcttc aaatagcgcg agtccgatgc gtcgccgacg
     1441 atgcagaagc ggtcgctgta gggcagtcgt gccgcgccga cggcgacgcg cggatggcag
     1501 tggcaatcga gtttccaatc cgggggcaga taatcgcgca tgtaggattc gcgcaggagc
     1561 ctctcggtgg agacgacatc cacgttctcg ccgatggccg tgacggtgac gtgctcgcct
     1621 ttcgggatca gagcgacgta gcgcacgtcc gcttgcttga ggacgaagaa atgtatgagc
     1681 ttacccagac gctcgcgctg aaactcttcg gagacgcgcg cttcgatcac gcacgtcttc
     1741 cacgtcggcg gcggttgata gccgtagatg aggcgcgcgc cgagttgcga gttgacgccg
     1801 aacgcgccga cgaccagatc gtattcctcg cgtccgcctt catgcgtgcg aatggcggga
     1861 cgctctgcga gcgtggcggg cacgtcgatg tcgtgtacgt tctgcgcgat gtgctgtgcg
     1921 ccgagtttga gggcttgatc gagcaggaat tgatcgaagc tgcgcgcgcc gtccgggtgt
     1981 tccgtgctga cgggtcctgc gccgcgatag acgccgtaaa ttttccgccc cggaacattt
     2041 tccagacgcg ccgaggcatc ctgataatgc acggcgtagc cttcgagttc gtagtggata
     2101 acctccgcag ggatgttcgt cgtcgcgtgc tcgatctctt cgatgaggtt gtggccgacg
     2161 gctccggcgc acatgttgca gccgggcgcg cccttgcgtg cgaagttttt gccgtcgtag
     2221 atgcagactt ggagggagag tccgagcgag cgtgcgcggc gcagcagttc gatggcgaag
     2281 aacgaaccgg cgggacctgc gcccactacc gcgatgcggc tccctgacgt gagataggtc
     2341 ggctgcgaca taacacctcg cgcacctcac acgctacctt tgacgaacgg gcggaattta
     2401 gacgaaggga agacagacgc ccggacgcat gcggcggggg aagacgctcc gactcgtcag
     2461 ccgacgcgtt aaaaattcaa cctgctgctt tcaatctcag tgctttcaat cttacgtcaa
     2521 agcataggcg tacgccggaa agcgtgtcaa cagcgaggcc gcgcgaaaca gatcgccgcg
     2581 ccgctttgcg cctgcaatat aatcctgaac ggaacagacg cccgcgccgc ctgcgcagca
     2641 gaatgcactt tagacgcttg cggctgcacg gcaagaggag gccaatgaat gatcattgtt
     2701 cgcgctgaaa cttcgaaaga cattgaggcc gtgcgccggg tcaaccagtt ggcgttcggg
     2761 cgggcggagg aagcgaatct ggttgacgcg ctgcgtctgg cggctcaccc gtatatctcg
     2821 ctcgtggccg agacgggagg gcgggtggcc gggcacatct tcttcagccc cgtcacgatt
     2881 gagacggaag ttcgcgcccc tgcgatcttc gggcttgcac cgatggcggt cttgcctgag
     2941 caccagaatc agggcatcgg ttcgcgtctg gtgcgtgaag gcttgaacga gtgccggagg
     3001 atcagcgtcg gcgcggtggt cgtcttggga catcccgcat actacccgcg tttcggtttc
     3061 gttccggcca gttcaaaagg gttgcgctgt gagtatccgg tgccggacga agccttcatg
     3121 gtgacggagt tgacgcccgg cgaactgccg tcgcaagggc tggtaaaata tcatcctgag
     3181 tttggcaacg tctgaggctt tgactcgcgt gtgtcggctg gcgtccggcg acgggcgatt
     3241 gacggaagga gaaaaatgtt tgttgccttg tatcgttgga agttgagaga gggagaggag
     3301 cagaggtttc gcgaaggctg gcgcaggcgc acagaggaga tttaccggaa gtgcggcagc
     3361 ttcggctctc gcctgcaccg tgcggaggac gggacgtggg tggcctacgc gcagtggcct
     3421 gacaggcgac actgggaagc ggcgcgacaa gtcggcgtca cagacacgca agctcctgcg
     3481 atgatgaagg agagcatcga agtgtcgtat ccggagatgc tgctggaggt ggcggacgac
     3541 ctgttcaaac cgcgcggctg tttagagacg ggggaatagt tgcgcggtac ttttatttgt
     3601 gaaaggtcgt tcgggaaacc gcctgtgaac agtgagggaa caaatccatc ggcgcggtgt
     3661 ctagtaactg ctcgtctaac tttttggtgc ggggaggctc gtcgtaatgg cactcgcgat
     3721 tctcaacatt ctggtagcgt tgtttctagg ctacggcgcg gtggaggagt tttgggtgag
     3781 aggcgtgcgg ggcggtgagt tgcaaccgct cgtcgtcggg ctggtggggg cgtctgtgag
     3841 cgtgctgctc gcgctggcgg gcgtcgggct atggcgtctc tggccgaacg cacgcgggct
     3901 ggccgtcgcg gcagccgtcc tctcaatcct gttccacgtc tatgcggcct tcccgccgca
     3961 ccgcaacgtg ggaattttag tcttgctcgt cggcgcgggt tatggattga tcttactgtg
     4021 catcacgctc acacaggcga cgcgcaaagc gcaggcggcc taagctcaca tggcgggcgg
     4081 gggcttgcgg cgcgagttcg aggttcaagt cgtgaagatg gaaacgggga ctctgataaa
     4141 agagtccccg ttccctgttt agctcaacgt gtagtccggc ggggttgatc aatcagccgc
     4201 cgtcgcttgc tgcggcgaag tttcgacgct gccgccgcct ccgatgcctt cggcgacgcg
     4261 tcgcccgtag tcttcatcac atttggtgag cagagagatc atgcgttcct gtatgtccgg
     4321 gttgcactgt ttgagcgcgc tcgcgagatt gttgacgaga tcgtcgcgtt cccaatcttc
     4381 catcgtgcgg taacgctcgc ccgcctgttt gtaatcgttc gtccggctga tcttttgccg
     4441 gacgaccgcg ccctgcacgt aaggggcgtg ctctttgccc gcgcgctgcg cttcctgtag
     4501 cccgccgcga ctgctcggct cgtagttgac gtgcggattc tcgcccgcct cgttgccgtc
     4561 cacgaaatag gccatctggc cgtcgcgctg gttcgtagcg acgtgcttct tgggacggtt
     4621 gatcggcagt tgcaggtagt ttgtgccgac gcgataacgc tgcgtgtcgg aataggagaa
     4681 cgtgcgccct tgcagcatct tgtcgtcgga gaagtcgagt ccatcaacta agacgcccgt
     4741 gccgaaagct acctgttcaa cttccgcgaa atagttttcg gggttgcggt cgagcaccat
     4801 gcgaccgacg ggcaacatcg ggaattgcac ctccggccag attttcgtgt cgtcgagcgg
     4861 gtcgaaatcg agttccgggt gctcgtcgtc cgacatgatc tgcacgcgca gttcccattc
     4921 ggggaagtcg ccgcgctcga tgacgtcgaa taaatctttc gtcgcgtggt tgaagtcgcc
     4981 tatcgcatcg gcctccgcct gcgtcaaaga gcgcacgccc tgcttcggaa tccagtgata
     5041 cttgacgagc acgcccaagc cctcggagtt caccatcttg taggtgttga cgcccgcgcc
     5101 ttcctgctct cgatagttgg ccgggatgcc gcgcgggctg aagagccacg agatcatgtg
     5161 catggattcg ggcgtgagac tgatgaagtc gaagatacga ttcgggtctt ggcggttcgt
     5221 cacggggtcg ggcttgaagg cgtgaaccac atcggggaat ttaagagggt cgcggatgaa
     5281 aaacactttc agattgttgc cgaccaaatc ccagttgccg tcttcggtgt agaacttcac
     5341 ggcgaagccg cgcgggtcgc gcaggttttc cggcgagtga ccaccgtgaa tgacggttga
     5401 gaagcgaacg aagaccggag ttctcttgcc cttctcctga aacaatttcg cgcgtgtgta
     5461 acggctcgcg ggttcgctgc cgatgcgccc gtaagcctcg aagtagccgt gcgcgcccgc
     5521 gccgcgcgcg tggacgacgc gttcggggat gcgctcgcgg tcgaagtgcg tgatcttctc
     5581 aaggaactgg tagttctcca gcaccgtcgg gcctcgctcg ccgaccgtgc gcgtgttttg
     5641 attgtcgtag acgggatgcc cctgccggtt cgtcaactcg ggctgctctt tcgcgccgcc
     5701 cgtcactccg ggcttcgtgc cgccgctctt ttcctcactc attagttctc tcctttcgcc
     5761 aggcgatagt aataattact attgctctgt tgtcaaaaaa aaattttacc ggcggcaatc
     5821 acggcaacgg cctaagattt ccaaacggta gtcgtgcacc tcaaaacccg cctgcggctt
     5881 cacacgttcc agccatgcag agcctttcac ggcgtcaagg tcccaaactc tgccacactc
     5941 tagacagcgt gtgtggtaat gatggtcggt gcggatatcg tagcgtgcgg aagcgtcgcc
     6001 gtaggtgagt ttcgatacca cctcgcactt gatgagagct tcgagatttt tgtagacggt
     6061 cgccagactg atgcgtggaa gtttcggctt gatggcgagg taaatctctt cggcggtcgg
     6121 gtgatgctcg acgccgctca gataatcgaa gacggcggcg cgctgcgatg taaggtgcac
     6181 gcccttctcg ccgagtgtct gccgcaaggc ttcgcgctcg cttattttct tctttgcagt
     6241 caaaccttga accgtccatt tcgtcgcaaa ctgcgcccat catggatcac gacgccacga
     6301 aagtcaatca taattattgt tttcaaaaca tgcccccggc gacgcgtgcg gaagcgcaca
     6361 ggaagacgct tcgcccgtca tgcgcgctga gcgttatact tccgcagcac gttcgccgaa
     6421 tctttagccg ggaattattg tgaaatgaga aa
//
LOCUS       DASWNS010000594         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_100348, whole genome shotgun sequence.
ACCESSION   DASWNS010000594 DASWNS010000000
VERSION     DASWNS010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100348"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VGB76_20430"
     CDS             <1..1167
                     /locus_tag="VGB76_20430"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0101302.1"
                     /translation="LEKDVIIIGGGAAGLFCAIEAGKRGRSVLVCEHAGQVGKKIAIS
                     GGGRCNFTNLHTSASNFISANPHFAKSALARYTPADFIALIERHAIAYHEKKLGQLFC
                     DGSSRQIINLLLAECSEAQVEIRCNCRVLRVERGDKFLLETDQGSFASRSLVIATGGL
                     SIPQLGATDFGYRIARQFDLRIEPTRPALVPLTLPTETQTRLVALSGVSLDAIARARE
                     AEFRENILVTHRGLSGPAILQVSSYWQPGEVIRLNLLPGADALELLLAQQHSEIELAN
                     LLGQYWPRRFAQEWCKLYAPSRPLNRFTERELDALAQRLQSWEITPAGTEGYKKAEVT
                     AGGVSTGELSSRTLEAKRVPGLYFIGEVLDVTGQLGGYNFQWAWASGYAAGQFV"
     gene            complement(1177..1575)
                     /locus_tag="VGB76_20435"
     CDS             complement(1177..1575)
                     /locus_tag="VGB76_20435"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEY0101303.1"
                     /translation="MRTLKLPSRYLFGLLFVLAGLYHFINPAFYLRMMPPYLPWHLFL
                     IYLSGCFEVAFGLLLFVPKYARVAAWGLIALLCAVFPANLYMAFNPQLFPDLPPVALW
                     LRLPLQAVFIAWAYWLTGSGRDRESRPNNS"
     gene            1583..1879
                     /locus_tag="VGB76_20440"
     CDS             1583..1879
                     /locus_tag="VGB76_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101304.1"
                     /translation="MQILLPLYDNEGRAFELQSYDRVRNELTKKFKGVTAFHNSPAEG
                     VWKQGGEVSRDRLVIFEVMSDELERDWWTEYRTELETRFRQERMVVRAILIELL"
     gene            complement(1905..3302)
                     /locus_tag="VGB76_20445"
     CDS             complement(1905..3302)
                     /locus_tag="VGB76_20445"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA Peptidase M64"
                     /protein_id="HEY0101305.1"
                     /translation="MKRILWLAILLTVCSLKTFAAPKTMRLDFYHTGNAAQELFSVDR
                     IVIEPLPWPGNPRRAIDETNLGKYLFEVREQKTNRLLYSRGFASIYGEWETTDEAKTV
                     NRTFQESLRFPAPDAPVQILLKKRDAKNSFREIWTTTVDPQNIFVSNAKIAPPGRVLE
                     IERNGEPSLKVDLLILGDGYTAAETAKFERDARRLTATLFQTSPFKERRKDFNVWALS
                     PAASESGISRPSTGVHRDSPLGATYDAFGSERYVLTFDNQAFRRTASFAPYDFVEILA
                     NNRTYGGGGIFNLYGTVAADSAWSDYVFVHEFGHHFAALADEYYTSAVAYAPATERIE
                     PWEPNVTALLDPKNLKWKDLVSPDTPLPTPWEKEAFESYSREIQRRRREIRSQNRPEQ
                     EMDALFREELAHEKELFARGKYANRVGAFEGANYEAKGFYRPQVDCIMFTRTDAFCAV
                     CRRAIERVIDMHSRP"
     gene            3526..>4052
                     /locus_tag="VGB76_20450"
     CDS             3526..>4052
                     /locus_tag="VGB76_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101306.1"
                     /translation="MQSLTLNTQGTRDEPLDPAAEVARLETLLSERQLELTTLQEEMH
                     KFKARYTQIVGSRLAELAEVEQAIREAEARLHGAPEDEPVEGEPGGATKTGKAATAET
                     LALGNSLRKLFWSVAKLFHPDHASDESEARRRHQIMVEASRAYREGDAESLSTLLGDE
                     ELQFYCTGTQANAPE"
BASE COUNT          872 a         1191 c         1220 g          769 t
ORIGIN      
        1 ctggaaaagg atgtcatcat catcggcggc ggcgcggcgg ggcttttctg cgcgattgaa
       61 gcggggaagc gcgggcgcag tgtgctggtc tgcgagcacg cgggacaggt cggaaaaaag
      121 atcgccatct ccggcggcgg gcgttgcaac ttcaccaatc tccacacgag cgcgagcaac
      181 ttcatctccg ccaacccgca cttcgccaag tccgcgctcg cgcgctacac gcccgccgac
      241 ttcatcgcgc tcatcgaacg acacgccatc gcttatcacg aaaaaaagct cggccagttg
      301 ttctgcgacg gcagttcgcg ccagatcatc aacctgctgc tcgccgaatg cagcgaggcg
      361 caggtggaga ttcgttgcaa ctgccgcgtg ctgcgcgtgg agcgtggcga caaatttctg
      421 ttggagactg atcagggaag tttcgcttcc cgctcgctcg tcatcgccac cggcggcctc
      481 tccatcccgc aactcggagc cacagacttc ggctaccgta tagcgcgcca gtttgatctg
      541 cgaatcgaac cgaccagacc cgcgctcgtg ccgctaacgt tgcccaccga aacgcagacg
      601 cgcctcgtcg ccctgagcgg cgtttcactc gacgccatcg cacgggcgcg cgaggccgag
      661 tttcgcgaaa acattctcgt cacacacaga ggcttgagcg gtccggcgat cctgcaagtc
      721 tcttcctact ggcaacccgg cgaagttatc cgtctcaatc tcttgcccgg cgcggacgcg
      781 ctggagttac tgctcgcaca acaacattcg gagatcgagt tggcgaatct gctcggacag
      841 tattggccgc gccgcttcgc gcaggagtgg tgcaaactct acgcgccttc cagaccgctc
      901 aaccgtttca ccgagcgcga actcgacgcg ctggcgcaga gacttcaaag ctgggaaata
      961 actccggcgg gaacggaagg ctacaagaaa gcggaagtga cggcgggcgg cgtctcgacg
     1021 ggcgagctat cctcgcgcac gcttgaggcg aagcgcgtgc ccggcctcta cttcataggc
     1081 gaagtgttgg acgtgacggg tcaactcggc ggttataatt ttcaatgggc gtgggcttcg
     1141 ggttacgccg ccgggcagtt tgtttagtcg gagttattag gaattattcg ggcggctttc
     1201 cctgtcgcgc ccgctgcccg tcagccagta agcccatgcg atgaacacgg cttgcagcgg
     1261 tagccggagc catagagcga cgggcgggag gtctgggaaa agttgcgggt tgaacgccat
     1321 gtagaggttt gcgggaaaca ccgcacagag caacgcgatc aaaccccacg ccgcgacgcg
     1381 cgcgtacttt gggacgaaca gcaacaggcc gaaagccact tcgaagcacc cgctcaggta
     1441 gatgaggaag agatgccagg gcaggtaagg cggcatcatc cgcaggtaga atgcagggtt
     1501 gatgaagtgg tacagcccgg ccagcacaaa caagaggccg aacaaatatc tcgacgggag
     1561 ttttagcgtt cgcatgtatc tgatgcaaat attactgccg ctctatgata acgaagggcg
     1621 tgcttttgaa cttcaaagtt atgatcgcgt gcggaatgaa ttgacgaaga aatttaaggg
     1681 cgtcacggcc tttcacaatt cgcctgcgga aggcgtctgg aagcagggcg gggaagtgag
     1741 ccgcgaccgc ctggtcatct tcgaagtcat gtcggacgaa cttgagcgcg attggtggac
     1801 agagtatcgc acggagttgg agacgcgttt ccgccaagag cggatggtgg tccgcgccat
     1861 cctgatcgaa ctgctttgac cgggcagcct tccgccgcga gtctctatgg tcttgagtgc
     1921 atgtctatca cgcgctcgat ggcgcggcgg cacacggcgc agaaagcgtc cgtgcgcgtg
     1981 aacatgatgc aatctacctg cggacgataa aaacccttcg cttcatagtt tgcgccctcg
     2041 aacgcgccga cgcggttggc atatttcccg cgcgcaaaca gctccttctc gtgcgcgagt
     2101 tcttcgcgaa agagcgcgtc catctcctgt tcgggtcggt tctggcttcg tatctcgcgc
     2161 ctgcgtctct gtatctcgcg ggaataactt tcaaaagcct ctttctccca cggagtcggc
     2221 agaggcgtgt cggggctaac gaggtctttc catttcaagt tcttaggatc gagcagtgcg
     2281 gtaacgttcg gctcccacgg ctcgatgcgt tcggtcgcag gggcataggc caccgccgac
     2341 gtgtaatact cgtcggcgag cgcggcgaag tgatggccga actcatgtac gaaaacatag
     2401 tccgaccatg cgctgtcggc ggcgaccgtg ccgtagaggt tgaagattcc gccgccgccg
     2461 taggtgcgat tgttggcgag aatctctacg aaatcatagg gagcgaaaga ggccgtgcgg
     2521 cgaaaggctt ggttgtcgaa ggtgagcacg tatctttccg agccgaaggc gtcgtaagtt
     2581 gcgccaagcg gcgagtcgcg atgcacgccc gtcgaggggc gcgagatgcc ggactcgctg
     2641 gccgcagggc tgagcgccca gacgttgaag tccttgcgcc gttctttgaa aggcgaagtc
     2701 tgaaacagcg tggccgtcag acgccgcgcg tcgcgttcga acttggccgt ctccgccgcc
     2761 gtgtagccgt cgccgaggat caagagatcg actttcaaag acggctcgcc gttgcgctcg
     2821 atctcaagca cgcgaccggg cggagcgatc tttgcgttgg agacgaaaat gttttgcgga
     2881 tcaactgtcg tcgtccagat ttcacggaac gaatttttgg cgtcgcgctt ctttaacagg
     2941 atttggacgg gcgcgtcggg cgcgggaaac ctgagcgact cctgaaaggt gcgattgacg
     3001 gtcttggcct cgtccgtcgt ttcccactcg ccgtaaattg aagcgaaccc gcgcgagtac
     3061 aacagccggt tggttttctg ctccctgact tcgaatagat acttgccgag gttcgtctcg
     3121 tcaatagcgc gcctcggatt gcccggccag gggagcggtt cgatcacgat tctatccacg
     3181 ctgaacaatt cctgcgcggc gttgcccgta tgatagaagt cgagccgcat ggtcttcggt
     3241 gcggcgaaag ttttcagcga acagaccgtc aacaggatcg cgagccacaa aattcttttc
     3301 ataagcctct ctcgaatttc tgatccgaac tacgacgcac acgcgccgct cgcgcgcaca
     3361 taaaatccca cacaacctct taccggcaac acagaataac cgaagacgcc tcccgctaca
     3421 taagcccgcc acacagcaac acatcctcag gcgtccgata acacctgagg atgatctggc
     3481 gagaagagtt tcagttcact attgggatac aatgcttcga cagctatgca gagccttact
     3541 ctaaacacgc agggaacacg cgacgagcca ctcgatccgg cggcggaagt ggcgcgtctt
     3601 gagacgctct tgtccgagcg gcaactcgaa ctcacaacgc tgcaagaaga gatgcacaag
     3661 ttcaaggcgc gctacacgca gatcgtcggc agtcgtctgg ccgaactggc agaggtcgag
     3721 caggccatca gggaggccga agcacgcctc cacggcgcgc ctgaagatga gccggtcgaa
     3781 ggggaacccg gcggcgcaac taaaacgggc aaggccgcga cggcggagac gttggccttg
     3841 ggaaacagcc tgcgcaaact cttctggtcg gtcgcaaaac tcttccatcc agaccacgcc
     3901 tccgacgaat cagaagccag acgccgccat caaatcatgg tggaagccag ccgcgcctac
     3961 cgcgagggcg acgcggaaag tctaagcacg ctgctcggcg acgaagaact gcaattctat
     4021 tgcaccggca cgcaagccaa cgccccggaa ga
//
LOCUS       DASWNS010000595         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_204807, whole genome shotgun sequence.
ACCESSION   DASWNS010000595 DASWNS010000000
VERSION     DASWNS010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204807"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VGB76_20455"
     CDS             <1..659
                     /locus_tag="VGB76_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101307.1"
                     /translation="TLAGDDRTLLGTHLDECLGCACVRDEIRTIVTTAAESREHFVAP
                     PNERALWLRVRNTIEAELTAQQRAIAANSFKAESAHENFLSRLMNKRWALSLPQLTAA
                     VVAIVIGVSVITTVGMQRLAAGEGGAKPVADVKSPPRLNSDQMIAIEYLMQRVEERKS
                     RWNPRMREAFDRNLGIIDATVSDSLKELKESPHDEISEEALNAALRDKMELLREFSEL
                     "
     gene            739..1560
                     /locus_tag="VGB76_20460"
     CDS             739..1560
                     /locus_tag="VGB76_20460"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY0101308.1"
                     /translation="MKKTQLIPHMPAAVCDAATDGSGRAMRRPLLVACAAALLLFVLS
                     PTATTFAQHKFSKTYPARRNIRLQLNNRSGTIEVVGWHRNEIKISADMEAPAARFTPI
                     LTGDGLVIDVMNDTRGREDVGDVNFRIQVPSESTVDLETKRGNIIVHNVQGAMVRAVV
                     TTEGDINLTGIRAHRVMASIISGNILFDADLMRGGMYELKSTQGDINLRINAGSGFTL
                     TATAPRTRNINLGDFAGRGSWDFQSNNRRVVGKVGDGGAILNTTNLRGSIMFVSR"
     gene            1647..2621
                     /locus_tag="VGB76_20465"
     CDS             1647..2621
                     /locus_tag="VGB76_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101309.1"
                     /translation="MHQHSNFYAVSGSRAPSSAQLTRASLFVILLSLLPCFLFFCAAQ
                     AQAQSQRNRGTASVAPTPTPAPAPQLTRSSTRQETRRFGYGGTLTIYGAPVGSLSIEA
                     WGRNEVEITAEIELKGGTEEELAALAAVNNFVLDEDGSHLTLVTTGTHDRKFMKRAAK
                     DFSKKLLTLPWKIDYRIRVPAQIDLEIYAGRGALDISNTDGAISLNAGESQAAFRLGS
                     GDVVSTIAGGSVSLRVPVNSWRGRGASIRLGRGDFNIELPANFSGDIDATVLRAGRIE
                     NNHPALAQRAGVASNARQLRARAGAGGATLLFEIGDGVIRINKASGDK"
BASE COUNT          549 a          903 c          768 g          456 t
ORIGIN      
        1 gcactctggc cggagatgat cgcacgctgc tgggcacaca tcttgacgag tgcctcggtt
       61 gcgcgtgtgt gcgcgacgag attcgtacca tcgtgacgac ggcggcggag agccgcgagc
      121 atttcgtcgc accgccgaac gagcgcgccc tgtggctgcg cgtgcgcaac accatcgaag
      181 ccgaactcac ggcgcagcag cgtgcgattg cggccaactc cttcaaggcc gagagcgcgc
      241 acgagaattt tttatcgcgc ttgatgaata agcgctgggc tttgtctctg ccgcaactga
      301 cggctgccgt ggtcgccatc gtcatcggcg tctcggtcat caccaccgtc ggcatgcagc
      361 gtctggcggc gggcgaaggc ggcgcaaagc ccgtggcgga tgtgaagtct ccgccgcgac
      421 tcaattcgga tcagatgatc gctatcgagt atttaatgca gcgcgtggaa gagcgcaaga
      481 gccgttggaa cccgcgcatg cgcgaagcct tcgaccgcaa cctcggcatc atcgacgcga
      541 cggtcagcga ttcgctcaag gaactcaagg aaagcccgca cgatgagatt tccgaagaag
      601 ccttaaacgc cgcgctccgc gacaagatgg aattgctgcg cgagttctcc gaactgtaag
      661 attgcatttc gcgtgtaagc gacatttccc gcgctgacgc cgtagcgaca ccccgaccag
      721 cgagagattt agagattgat gaagaagacg cagttgattc ctcacatgcc cgccgcagtg
      781 tgcgatgccg cgacggatgg gtcggggcgt gcgatgcgac ggcctctact cgtggcctgc
      841 gccgccgcgc tcctgctgtt cgtcctctcg ccgaccgcca caactttcgc gcaacacaaa
      901 ttctccaaga cttacccggc gcgccgcaac atacgtctgc aactcaacaa tcgttcgggc
      961 acgattgaag tcgtcggttg gcatcgcaac gagatcaaaa tctccgccga catggaagcg
     1021 cccgccgcgc gcttcacgcc catcctgacg ggcgacggcc tcgtgatcga cgtgatgaac
     1081 gacacgcgcg ggcgcgaaga tgtcggcgat gtcaactttc ggattcaagt gccgtccgaa
     1141 tccacggtcg atctcgaaac caagcgcggc aacatcatcg tgcataacgt gcagggcgcg
     1201 atggtgcgtg ccgtcgtgac caccgaaggc gacatcaacc tgacaggcat ccgcgcccat
     1261 cgcgtgatgg cttccatcat cagcggcaac atcctgttcg acgcagacct catgcgcggc
     1321 ggcatgtacg aactcaagtc cacacagggc gacatcaacc tgcgcatcaa cgcaggctcc
     1381 ggcttcaccc tgacggcgac cgcgccgcgc acgcgcaaca tcaatctcgg cgacttcgcc
     1441 gggcggggca gttgggactt tcagagcaac aaccgccgcg tcgtcggcaa agtgggcgac
     1501 ggcggcgcga tcttgaacac gacgaacctg cgcggctcca tcatgttcgt ctcgcgctga
     1561 gcgatagact cctgcacgcg ctaagcacgc gcgcgctgcg accgttcgac gctccccgca
     1621 tcttctcttc gaggcacacg ctccttttgc atcaacactc aaacttctac gccgtctccg
     1681 gctcacgcgc accttcaagc gcgcagctaa cgcgcgcttc tctttttgtc atccttttgt
     1741 ccctgctgcc ctgcttcctc ttcttctgcg ccgcccaagc acaagcgcaa tcgcaaagaa
     1801 acaggggaac ggcaagcgtc gcacccacgc cgacgcccgc gcctgcgccg caactcacgc
     1861 gtagttccac gcgccaggaa acgcgtcgct tcggctacgg cggcacgctc acgatctacg
     1921 gcgcgccggt cggctcgctc tccatcgaag cgtgggggcg caatgaggtg gagatcaccg
     1981 ccgagatcga actcaaaggc ggcacggaag aagaactcgc cgcgctcgcc gccgtcaaca
     2041 atttcgtgct cgacgaagac ggctcgcacc tcacgctcgt caccaccggc acgcacgacc
     2101 gcaagtttat gaaacgcgcg gccaaagatt tctccaaaaa acttctcacg ctgccgtgga
     2161 aaatagacta ccgcatccgc gtccccgccc agatcgatct ggaaatttac gccgggcgcg
     2221 gcgcgctcga catcagcaat accgacggcg cgatcagtct caacgcggga gagagccagg
     2281 ccgcctttag gctcggcagc ggcgatgtcg tctccacaat cgcgggcggc tccgtctctc
     2341 tgcgcgtccc ggtcaacagt tggcgcgggc gcggcgcgtc catacgcctc ggtcgcggcg
     2401 acttcaacat cgaacttccc gccaacttca gcggcgacat cgacgccacg gtgctgcgag
     2461 ccggtcgcat cgaaaataac catcccgccc tcgcccagcg cgcaggcgtc gcctccaacg
     2521 cgcgtcaact ccgcgcccgc gccggagccg gaggtgcaac cctcctcttc gagatcggcg
     2581 acggcgtcat ccgcatcaac aaagcgagcg gcgacaagtg acaagcagag ccgcgaaacc
     2641 gtgaatcgtg aatcgtaaat cgtgaataga agtgaa
//
LOCUS       DASWNS010000596         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_22719, whole genome shotgun sequence.
ACCESSION   DASWNS010000596 DASWNS010000000
VERSION     DASWNS010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22719"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..970)
                     /locus_tag="VGB76_20470"
     CDS             complement(71..970)
                     /locus_tag="VGB76_20470"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101310.1"
                     /translation="MHTLLLFVDGLGIGTHGAHNPLDQLRETAAPLAIFADEEPELPG
                     GGLLVRTDACLGVEGRPQSASGQTTILTGINAPALLGYHKQGFPNEAMREIIREHSVF
                     LQLRRAGIEPNTFANAYTPRFFQTRPRWVSATTVAVEAAGMRFRELEDLRRGQAVYQD
                     FTNGLVIEAGFDVEPRTPEQAAAALAALVAAHRFTLYEYFITDRVGHAQDMEAALTVL
                     ENLARFVRAVIACVDLSDTTVLLTSDHGNIEDLSIRNHTRNFVPTLLWGARRREAASR
                     IRTLADITPAIIETLTAREATHA"
     gene            1130..>3528
                     /locus_tag="VGB76_20475"
     CDS             1130..>3528
                     /locus_tag="VGB76_20475"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEY0101311.1"
                     /translation="MTSFRKLCAHALTLVVALTHAPATLHAQDDKAVTKSAPQAVAPR
                     APQPVPYFSEPSISPDRTEIAFVSGGDIWTVPLAGGEARLLVSHPATEARPVYSPDGR
                     RIAFTSNRTGNGDIYVLSFDTGELRRVTFDDMGDQLDAWSRDGRWLYFSSISRDISGM
                     NDIFRVSPEGGTPLQVSADRYTNEFFSAPSPDNSAIAFTARGISSGQWWRKGRSHIDE
                     AEIWVRHEVNGAGVSASTYQRITPGGAKEMWPMWGADGDNIFYVSDRSGAQNIWLRPA
                     LRQARDGDAAAARQLTKFTDGRVLWPSISTDARVIVFERNFRIWKLDTSNNQASEVQI
                     TRRGAVAEPGVEHLRLTDRIDEFALSPDGKKVAFVVRGEVFAASASDGGDAARITVNP
                     ADESQVAWSHDSRRLVYVSNRDGTPHLFIYDFGTNAETQLTRAAEADATPRFSPDGRL
                     LAFERAGRELRVLNMETKAERVVATGNFERPPINPERPFVWSPDSRFIAFMPVASKLF
                     RNVYVVRADETSGAVVPVAQPVSFLANVFSNTVSWSPDGTFMLFDTGQRTENFQVARV
                     DLIPRTPRFREDQFRELFREEPPRNLTPPQRETNPPAPSPTATPTPAASPSPTPSPSP
                     AASPANSSADERKKPGDKKTEITFEGIRQRLSLLPVGLDVGYQAISPDGKWVVLIASA
                     AGQQNLYLYSLDELSREPAVPKQLTSTPGFKGLAQFSPDGKEIFYIEQGRLNVVTLEG
                     KTRALPVTAEMDVDFAREKMEVFRQVWSYLQEHFYDPNFHGVNWNAMRADYEPRIAGA
                     RT"
BASE COUNT          630 a         1118 c         1132 g          648 t
ORIGIN      
        1 ttcggcttac ggtgtcgtgc ggcgtctcga tgcgggaggt cggcgttcaa gtcgtgcgcg
       61 gcggtgtcgc tcatgcgtgc gtggcctctc tggctgtcag ggtttcgatg atggcgggcg
      121 tgatgtctgc gagcgtgcgg atgcggctgg cggcctccct ccggcgcgcg ccccagagaa
      181 gggtcggcac gaagttgcgc gtgtggttgc gtatgctcaa gtcttcgatg ttgccgtggt
      241 cgctcgtgag caggacggtc gtgtcggata agtcaacgca ggcgatgacg gcgcggacga
      301 agcgcgcgag attctctaag accgtgagcg cggcctccat gtcctgcgcg tggccgacgc
      361 gatctgtaat gaaatattcg tagagcgtga agcgatgcgc ggcgacgagc gcggccaacg
      421 ctgcggctgc ctgttcgggc gtgcgcggct ccacgtcgaa gcccgcttcg atgaccagcc
      481 cgttggtgaa atcctgatag actgcctgcc cgcgccgtaa atcttcaagt tcgcgaaagc
      541 gcatgcccgc cgcttcgacg gcgaccgtcg tggccgatac ccagcgcggt cgcgtctgga
      601 agaagcgcgg cgtgtaagcg ttggcgaaag tgttcggctc gattccggcg cgccggagtt
      661 ggagaaagac ggaatgctcg cggatgatct cgcgcatggc ttcgttggga aagccctgct
      721 tgtgatagcc gagcagggcg ggggcgttga tgcccgtcag gatcgtggtc tgcccggacg
      781 cgctctgcgg gcggccttcg acgccgaggc aggcatccgt gcgcacgagt agcccgccgc
      841 cggggagttc cggttcttcg tctgcgaaga tggcgagcgg cgcggctgtc tcgcgtagtt
      901 ggtcgagcgg attatgcgcc ccgtgcgtgc cgatgccgag gccgtcaacg aagagaagta
      961 gcgtgtgcat cgagggagat agtagcacgc ccgccaattc ccgccgcgcg ccgaatctct
     1021 gttatattga aaactcgaaa gccttcagac acgtttcaca cccttacgat tttgtacttt
     1081 gaaactgccg tcaataaatt tttccaaagc tctcctgccg gaggaacgaa tgacttcttt
     1141 tagaaaactg tgcgcgcacg cgctgacgct cgttgttgca ctcacccacg ctcccgcgac
     1201 attgcatgca caggacgata aggccgtgac gaagagcgcg ccgcaggccg ttgcgccgcg
     1261 tgcgccgcag cccgtgccct atttctccga gccttccatc tcgcccgacc gcacggagat
     1321 cgcgttcgtc tccggcggcg acatctggac tgtcccgctt gcgggcggcg aagcgcgcct
     1381 gctcgtctct caccccgcca ccgaagcgcg ccccgtctat tcgcccgacg gacgccgcat
     1441 cgccttcact tcgaatcgca cgggcaacgg agacatctac gtgctctcgt tcgacacggg
     1501 cgaactccgg cgcgtgacgt tcgacgatat gggcgatcaa ctcgatgcgt ggtcgcgcga
     1561 cgggcgctgg ctctacttct cttcgattag ccgcgacatc tccggcatga acgatatttt
     1621 tcgcgtcagc cccgaaggcg gcacgccctt gcaggtgagc gcggatcgct acacgaacga
     1681 atttttctcc gcgccgtcgc cggacaactc cgcaatcgcg tttacggcgc gcggcatctc
     1741 ctccggtcaa tggtggcgca aaggccgcag ccacattgac gaagcggaga tctgggtgcg
     1801 gcatgaggtg aacggggcgg gcgtctccgc gtcaacttat caacgcatca ctccgggcgg
     1861 cgcgaaagag atgtggccga tgtggggcgc ggacggcgac aatatctttt acgtctccga
     1921 ccggagcggt gcgcagaaca tctggctgcg tcccgcgctc aggcaggcgc gagacggaga
     1981 cgcggcagcc gcacgacaac tgacgaagtt tacggatggg cgcgtgctct ggccaagcat
     2041 ctccacggat gcacgcgtga tcgtcttcga gcgcaacttc cgcatctgga aactggacac
     2101 ttcgaacaat caggcgagcg aggttcagat cacacggcgc ggcgcggtgg ccgaaccggg
     2161 cgtcgagcat ctgcgtctga cggatcggat agacgaattc gcgctctcgc ccgacggcaa
     2221 gaaggtggcc ttcgtcgtgc gcggcgaagt gttcgccgcc tccgcgtccg acggcggcga
     2281 cgccgcgcgc atcaccgtca acccggcgga cgaatcgcag gtcgcctggt cgcacgacag
     2341 ccgccgtctc gtctacgtct ccaaccgcga cggcacgcca catctcttca tttatgattt
     2401 cggcacgaac gccgagacgc agttgacgcg cgccgccgaa gccgatgcca cgccgcgctt
     2461 ctcacccgat gggcgtctgc tcgccttcga gcgcgcgggc agggaactgc gcgtcttgaa
     2521 catggagacg aaagcggagc gcgtggtggc gacggggaat ttcgagaggc cgccgatcaa
     2581 tcctgagcgg cctttcgtgt ggtcgccgga cagccgcttc atcgcgttca tgccggtcgc
     2641 ttcgaagttg tttcgcaatg tctacgtcgt gcgcgcggat gagacgagcg gggcggttgt
     2701 gcccgtcgcc caacccgtca gctttctggc gaacgtcttc agcaacactg tgtcatggag
     2761 tcccgacggc acgttcatgc tgttcgatac gggacagcgc acggagaatt ttcaggtggc
     2821 gcgcgtcgat ctcatcccgc gcacgccccg tttccgcgaa gaccagtttc gtgaactctt
     2881 ccgcgaagag ccgccgcgca acttaacgcc gccgcagcgc gagacgaacc cgcccgcgcc
     2941 ttcgccgaca gcaacgccca cgcccgcagc ctcgccgtct cccaccccaa gcccgtcgcc
     3001 cgcagcctcg cccgccaaca gcagcgcgga cgagcgtaag aagccgggcg acaaaaagac
     3061 cgagatcact tttgaaggca tacgccagcg gctcagcctg ttgcccgtcg gcctcgacgt
     3121 gggttatcag gcgatcagtc ccgatggcaa atgggtcgtg ctgattgcga gcgcggcggg
     3181 acaacagaac ctctacctct attcgctcga cgaactctcg cgcgaaccgg cggtgccgaa
     3241 gcagttaact tccacgccgg gcttcaaagg gctggcgcag ttttcgcccg acggcaaaga
     3301 gatcttctac atcgagcagg gacgcttaaa cgtcgtcacg ctcgaaggca agacccgcgc
     3361 gctcccggtc acggcggaga tggacgtgga ttttgcgcgc gagaagatgg aagttttccg
     3421 gcaggtctgg tcgtatctgc aagagcattt ctacgacccg aattttcatg gcgtgaactg
     3481 gaacgcaatg cgcgccgact acgagccgcg catcgcgggc gcacgcac
//
LOCUS       DASWNS010000597         6937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_515609, whole genome shotgun sequence.
ACCESSION   DASWNS010000597 DASWNS010000000
VERSION     DASWNS010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515609"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VGB76_20480"
     CDS             <1..554
                     /locus_tag="VGB76_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101312.1"
                     /translation="SPILIDVSGNGYSMTGSTGGVRFDFNGDGVEAGQISWTAAGSDD
                     AWLVLDRNNNGRIDNGRELFGNATVQPVPPAGEERHGFLALAEYDRGENGGNGDWAID
                     SRDAVYSALRLWQDTNHNGISEPGELHSLPSLDVASIDLIYKESKRADEYGNEFRYRG
                     KVRDAKGAKVNRWAWDVFLVPAP"
     gene            746..1126
                     /locus_tag="VGB76_20485"
     CDS             746..1126
                     /locus_tag="VGB76_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101313.1"
                     /translation="MKRNSIISAPVVLLRPDAVTKAATPTGAPPPFDVTHYDAQVEPD
                     ITKKTVTGNVLIRIIARADNPATVEFDCGDLSIDAVRVNGEEQKFLRREGRLNVTLSR
                     PAKAARRCYGSLYSGTTGRRCYKI"
     gene            1130..2092
                     /locus_tag="VGB76_20490"
     CDS             1130..2092
                     /locus_tag="VGB76_20490"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY0101314.1"
                     /translation="MPRLNLPLIADAAQYESHFKDGVWREAAAAICARHGLPHNELRR
                     APQGENIIFFGDERFAVKIFAPFRESYARETAALEFALDKIDIKTPGILYKGELEGWS
                     YLVLTRLKGLPATEVWAEVESHERLEIVARLGAALKSLHGHGAPLSAPALNRDWRGFI
                     ERQAETCVARQRACGANAGWLERLPAYVAARLELLPAGYKPVLLHGDVHLGNLLLARN
                     KGGWEITGLLDFGDSFCGFHEYDFVAPGVLMLQGNRELQRAFLLAYGYRESHLDMNLR
                     ARLMLLTVLYECSNLRKYALRLKPESVHYTLDELEAAIWTFAAE"
     gene            complement(2209..2868)
                     /locus_tag="VGB76_20495"
     CDS             complement(2209..2868)
                     /locus_tag="VGB76_20495"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY0101315.1"
                     /translation="MDNHIRELAERLWHYHRLRHRLERAEAILVLCSYDLRVAERGAQ
                     LFLEGWAPLLIFSGGLGTITKGLWSEPEADQFARVAARMGVPEERILVENRSTNTGEN
                     IIFTKALLAEKRLDPAKFILVQKPYMERRSYATFRKMWPEKEVIVTSAQVSFEEYLEQ
                     YTNRELSSDEVVSIMVGDLQRIRVYPEKGYQIRQEIPDDVWSAYEELVKAGYDKHLVS
                     S"
     gene            complement(2888..3754)
                     /locus_tag="VGB76_20500"
     CDS             complement(2888..3754)
                     /locus_tag="VGB76_20500"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY0101316.1"
                     /translation="MSKHSPGTLNVACVQMHWAQSIEYNVERTEHYVQAAAESGARVV
                     LFPEANLTSYYFPYVVELQPKVVEEALERARAAAAAADLWVIVGTIKKTPDRFLNLMH
                     VIDPKGAIVHEYAKINTAGRDEKKYCRGGNKLSLFEIDGILCTMVICRDGRHPELYRL
                     PAMAGAQVLFQPSCSSDEIEAVAWKRISGRAQQPVGPNTKIFHCVANTIGQSPDGKQT
                     SSGASFIREPNGIPLAEAGYYQEEMISAVLDMSRADRSYVIDSMNNPPFLRRYWQEMI
                     EEMKTRVDSANE"
     gene            complement(3955..5124)
                     /gene="xylA"
                     /locus_tag="VGB76_20505"
     CDS             complement(3955..5124)
                     /gene="xylA"
                     /locus_tag="VGB76_20505"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY0101317.1"
                     /translation="MSQDTYTPRPEHKFTFGLWTVGNRGRDPFGDAVRETLAPVDAVR
                     LLAETGAYGVNFHDNDLVPIDADAHERDRIVSEFRAACETHGLKVPMATVNLFYDAAF
                     RDGAFTANDAAVRAYALQKTCRAMDLGAEFGAQVFVLWGGREGTETDACRNPADAVKR
                     FRAALNYLCEYSISQGYGYKFALEAKPNEPRGDIYFPTTGAYLALIPTLDHAEMVGVN
                     PEFAHETMAGLNVTHAVAQAWEAGKLFHIDLNDQIPGRYDQDFRFGSASPRQAFYLVK
                     FLEDVGYEGMRHFDSHAYRTEDYDGVRDFARGSMRTYLILKDKARRWNEDAEIQSLLA
                     EINEGVSGIPATGSFDKERHARLDAHAFDRSALAGKRLPYERLDQLTLEILLGVR"
     gene            complement(5183..6688)
                     /gene="xylB"
                     /locus_tag="VGB76_20510"
     CDS             complement(5183..6688)
                     /gene="xylB"
                     /locus_tag="VGB76_20510"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEY0101318.1"
                     /translation="MKLLGIDVGTGGTRAVVVDETGRVAAAATSEHAPFVSPQTGWAE
                     QDARDWWRAAGEAVRAVLACGEVRADEIECVGLSGQMHGATLLDERDEVLRPSIIWCD
                     VRTGEQCRALTSQIGETRLIELVLNPALANFTLPKMLWVREREPHLWERVRTVLLPKD
                     YVRLRLTGERATDVADASGTLLFAVGERAWSNEILERTGMERSLLPAVYESPEVCGRV
                     SAAGAAATGLRAGTPVVAGAGDQAAGAVGLGVVRPGSVSATIGTSGVVFAATDRPALD
                     LKGRVHTFCHAIPGRWHVMGVTQAAGLSLRWFRDRFGTGAAGASAGDGRDAYEILSEE
                     ADGAPPGADGVLWTPYLMGERTPHLDPQARAALVGLTMNHTRAHIIRAILEGVAFSLR
                     DTLTIFAEMNVPVRGIKLGGGGARSALWRQIQADVYGQTIELVEAEEGAAYGAALLAG
                     VGGGAWSSVDEACEATVRVRARVEPDAARVSLMNERYGVYRAIYPALRDVL"
     gene            complement(6721..>6937)
                     /locus_tag="VGB76_20515"
     CDS             complement(6721..>6937)
                     /locus_tag="VGB76_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0101319.1"
                     /translation="GIAAAVLGGCSLRGGEGSIIGILLGTALLQVLQNLVNLLGIPSS
                     LNFAVMGGVILIGVIADQMLQRRTARA"
BASE COUNT         1328 a         2197 c         2032 g         1380 t
ORIGIN      
        1 tctcgcccat cctcatagac gtatccggca acggttattc gatgacgggt agtacgggcg
       61 gcgtgcgctt cgacttcaac ggcgacggcg tggaggcagg tcagatatcg tggacggcag
      121 ccggttcgga tgatgcctgg ctcgtcctcg accgcaataa taacggcagg attgacaacg
      181 ggcgggaact cttcggcaat gctactgtgc agcctgtacc gcctgcggga gaggaacggc
      241 acgggttcct cgcactcgcc gaatatgaca gaggggagaa tggcggcaac ggtgactggg
      301 cgattgatag cagagatgcc gtctattcag cactcaggct atggcaggac acgaaccaca
      361 acggcatctc tgagccggga gaactgcaca gcctcccgtc attggatgtc gcctcgattg
      421 atctcattta caaagagtct aagcgagccg atgaatatgg caacgagttc aggtatcgcg
      481 ggaaggtgag agatgcgaaa ggtgcgaagg taaatcgctg ggcttgggac gtgttcctcg
      541 taccagcgcc atgaccgctc cttgatctat gtcaacagct taccccgccc ctcagtctgt
      601 ttaactgagg ggcgggtcct ttttagacgc agccgtgatt ttgccgggag cgaaaatgat
      661 ccgtcacgtc gtccgcgcag caggcaccgt ttgatctgtc tgccgggcgg ggctataact
      721 ttctttcccg aagcgcgtga cacacatgaa gcgtaacagc attatttctg ctcctgtcgt
      781 tctactgcgc cctgacgccg tgaccaaagc ggctacgccg acaggcgcgc cgcccccctt
      841 tgacgtaaca cactatgacg cgcaggtcga acctgacatc acgaagaaga cggttacggg
      901 aaacgttctg atacggatca tcgcacgcgc ggataatccg gcgacggtcg agttcgactg
      961 cggcgacctc tcgatagatg ccgtgcgcgt gaacggggag gagcaaaaat tcctgcgccg
     1021 ggagggtcgt ttaaatgtca cgttgtcgcg tccggcgaaa gctgcacgcc gctgctacgg
     1081 ttctctttac agtggaacaa ccggcaggcg ttgctataaa atttgagcga tgccgcgctt
     1141 gaacctgcct cttatcgccg acgctgcaca gtacgagtct cattttaaag acggcgtgtg
     1201 gcgagaagcc gccgccgcca tctgcgcccg gcatggtctt cctcacaacg agttgcggcg
     1261 cgcgccgcag ggcgaaaaca tcatcttctt tggcgacgaa agattcgctg tcaaaatttt
     1321 cgccccgttt cgtgaaagct acgcgcgcga gacagcagcc ctcgaattcg ccctcgacaa
     1381 aatcgatatt aagacgccgg ggattctata caagggggaa ctcgaaggtt ggtcgtatct
     1441 cgtcttgacg cggctcaagg ggcttccggc gacagaggtg tgggcggagg tcgaatcgca
     1501 cgaacgtctc gaaatcgtcg cgcgtctcgg ggctgcgctg aaaagtctgc acgggcacgg
     1561 cgcgccgctc tctgctcccg ctctcaatcg tgactggcgc gggttcattg agcgacaggc
     1621 tgaaacgtgc gtcgcgcgcc agcgagcctg cggcgcaaat gccggatggt tggagaggct
     1681 gcccgcttat gttgccgcga ggcttgagtt actaccggca gggtataaac ctgtgctgct
     1741 tcatggcgac gttcatctcg gcaacctgct gctcgcgcgg aacaaaggag gctgggagat
     1801 cacgggtctg ctggatttcg gagactcgtt ttgcggtttt cacgaatacg attttgtcgc
     1861 gcccggcgtc ctcatgctgc aaggaaaccg cgaattacag agggcatttc tgctcgctta
     1921 cggttatcgg gaatcgcacc tcgatatgaa cctgcgcgcc cgcctgatgc tgctgaccgt
     1981 tctctacgaa tgcagcaatt tgcgtaagta cgctttgcgt ctgaagcccg aatccgttca
     2041 ctacacgctc gacgaactgg aagccgcgat ctggactttc gcggcagagt gataacagcg
     2101 acgcaggcgt gccacggcag acccggttcc ggaaaaccgg aaagattccg cgcccctatg
     2161 cttcataacg atctctggtt caggggaggc gagagcgaaa gttatcagtt aagaactgac
     2221 gagatgtttg tcatatccgg ccttgaccaa ctcttcatac gccgaccaga catcgtccgg
     2281 aatctcctgt cgaatctggt atcccttctc cggatacact ctaattcgct gcaaatcccc
     2341 gaccatgatg ctgaccactt catcgctcga taactcgcgg tttgtatact gctcaagata
     2401 ttcttcaaac gagacttgag ccgaggtgac gatgacctct ttttccggcc acatctttct
     2461 gaacgtcgcg tagcttctgc gctccatgta cggcttttgc acgaggatga actttgcggg
     2521 gtcgagcctc ttctccgcca gtagtgcctt tgtaaatatg atgttctcgc ccgtgttcgt
     2581 cgatctgttc tcgaccagta tccgctcctc cggcacaccc atccgcgcgg cgacccgggc
     2641 aaactgatct gcttccggtt cgctccacaa acctttggta atcgtcccca gcccgcctga
     2701 aaagatgagt aaaggcgccc agccttccag aaacaactgc gcgccgcgct ccgccacccg
     2761 caggtcgtaa ctacagagaa cgaggatcgc ttccgcccgt tcgagtcgat gtctcagccg
     2821 gtggtagtgc cagagtctct ccgccaactc tcgtatgtga ttgtccatcg ccctcgaaga
     2881 agcgcgttca ctcgttcgcc gagtccacac gcgtcttcat ctcctcgatc atctcctgcc
     2941 agtatctacg gaggaacggc ggattgttca tgctgtcgat cacgtatgaa cggtctgcgc
     3001 gcgacatatc cagcaccgcc gagatcatct cttcctgata gtaaccggct tcggccagag
     3061 ggatgccgtt cggttcgcgt ataaaggagg cgccgctgga agtctgcttc ccgtcaggcg
     3121 actggccgat ggtgttcgcc acgcaatgga agattttcgt gttcggcccg acgggctgtt
     3181 gcgcccgccc ggagatgcgc ttccaggcca ccgcttcgat ctcatccgaa ctgcacgacg
     3241 gctgaaagag cacctgcgcc cccgccatcg caggcagacg gtacaactcc gggtgccgcc
     3301 cgtcgcggca gatcaccatc gtgcagagaa tcccgtctat ctcgaacagc gataacttgt
     3361 tgccgccccg acaatacttc ttctcgtcgc gtcccgccgt attgatcttg gcatactcat
     3421 gcacaatcgc gcccttcgga tcgataacgt gcatgagatt caggaaacgg tcgggggtct
     3481 ttttgatggt tcccacgatg acccacaggt cagccgccgc cgctgccgcg cgagcgcgtt
     3541 ccaacgcttc ctcgacgacc ttcggttgaa gctctacgac gtaaggaaag taatagctcg
     3601 tcaaattcgc ttcgggaaaa agtacgacgc gcgccccgga ctcggcggcg gcctgtacat
     3661 agtgttccgt tctttcaacg ttgtactcga tgctttgcgc ccagtgcatc tgcacgcagg
     3721 ctacgttcag agtgccggga ctgtgtttag acataatctc tcattctcct tgccgcaata
     3781 aacctctttg ccgacgccgg gacgcgaggc cgccccactc ctgcctataa tccggaacat
     3841 ctttcctcca gagactttca aggccaagct cgaacgcgcc gcaatgacct gtcgcccggg
     3901 cgacggctcc gctgcggcaa cggcggagcg tccggctgat gtttgaaggg gaaattagcg
     3961 aacgcctaaa agaatctcaa gggtcagttg gtcaagccgt tcgtagggca ggcgtttccc
     4021 tgcgagcgcg ctgcggtcga aggcgtgtgc gtcgaggcgc gcgtgccgtt ctttgtcgaa
     4081 gctcccggtc gcagggatac cggagacgcc ttcgttgatc tccgcaagca gcgactggat
     4141 ttccgcgtcc tcgttccagc ggcgcgcttt atctttcagg atgagatagg tgcgcatgct
     4201 gccgcgcgcg aagtctctaa ccccgtcgta gtcttcggtg cggtaggcgt gagagtcgaa
     4261 atgtctcatc ccttcgtagc cgacatcctc cagaaacttg acgaggtaga acgcctgtcg
     4321 cgggctggcg gaaccgaagc ggaaatcctg atcgtaacga cccggtatct ggtcgttcag
     4381 gtcgatgtga aagagtttgc ccgcttccca cgcctgcgcg acggcgtgtg tgacgttcaa
     4441 gcccgccatc gtctcgtgcg caaactccgg attgacgccg accatctctg catgatcgag
     4501 cgtcgggatg agcgcgagat acgcgcccgt cgtcgggaag tagatgtcgc cgcgcggttc
     4561 gttgggtttg gcttcgagcg caaacttgta accgtagccc tgcgagatgg aatactcaca
     4621 caggtagttg agcgccgcgc ggaaacgctt cacggcgtcg gcaggattgc ggcacgcgtc
     4681 cgtctccgtg ccctcgcgcc cgccccacag cacgaacacc tgcgcgccaa actctgcgcc
     4741 caaatccatc gcgcgacacg tcttctgcaa agcgtaggcg cgcaccgccg cgtcgttcgc
     4801 cgtgaacgcg ccgtcgcgga aggctgcgtc gtaaaagagg ttgacggttg ccatcggcac
     4861 tttcagcccg tgcgtctcgc aggccgcgcg aaactccgag acgatccggt cgcgctcgtg
     4921 cgcgtcggcg tcaatcggca cgagatcgtt gtcgtgaaag ttgacgccgt aagctccggt
     4981 ttcggcgagc aggcgcacgg cgtcaacggg cgcgagcgtc tcgcgtacgg cgtcgccgaa
     5041 agggtcgcgc ccgcgattcc cgaccgtcca cagtccgaag gtgaacttat gttccgggcg
     5101 cggcgtgtat gtatcttggc tcattattcc caccttatca tctcagtaaa tttggatggc
     5161 tcgatcaagc gaaggctgaa ctttacaaca catcgcgcag cgccgggtag atggctcgat
     5221 agacgccgta tcgttcgttc attaaactca cacgcgccgc gtcaggctca acccgcgcac
     5281 gcacgcgcac ggtcgcctcg cacgcctcgt caacggaaga ccacgcgccg ccgcccacac
     5341 ctgcgagcaa cgccgcgccg taggccgcgc cttcttccgc ttcgaccaac tcaatcgtct
     5401 gcccgtagac atcggcctga atctgccgcc acagggcgga acgcgcgccg ccgccgccga
     5461 gtttgattcc acggacgggc acgttcatct ccgcgaaaat agttagcgtg tcgcgcaggg
     5521 agaaggcgac gccttcgagt atggcgcgga tgatgtgcgc gcgtgtgtgg ttcatcgtca
     5581 gaccgacgag cgcggcgcgc gcctgcgggt cgaggtgcgg cgtgcgctcg cccatcagat
     5641 acggagtcca gagcacgccg tcggctcccg gcggcgcacc gtctgcctcc tcgctcaata
     5701 tctcgtaagc gtcgcgcccg tcgcccgccg acgctcctgc tgcgcccgtg ccgaagcggt
     5761 cgcgaaacca gcgcaggctg agtccagccg cctgcgtcac gcccatcaca tgccagcgtc
     5821 cggggatggc gtgacagaag gtgtgcacac gtcccttgag gtcaagcgcg ggtcggtcag
     5881 tcgcggcgaa gactacgccg gacgtgccga tggtcgcact gaccgaaccg gggcgcacca
     5941 ctcccaagcc cactgccccc gccgcctgat cgcccgcgcc cgccacgacg ggcgtgcccg
     6001 cgcgcaaccc cgtcgccgcc gcccctgccg cagacacgcg cccgcacact tccggcgatt
     6061 cgtagacggc gggcagcagg ctccgctcca tgcccgtgcg ttcgagtatt tcgttcgacc
     6121 acgcgcgctc cccgacggcg aagagcagcg ttcccgaagc atcggccacg tccgtcgcgc
     6181 gttcgcccgt caggcgcagc cgcacgtaat ccttcggcag cagcaccgtc cgcacgcgct
     6241 cccaaagatg cggctcgcgt tcgcgcaccc agagcatttt ggggagcgtg aaattagcga
     6301 gcgcggggtt taagacgagt tcgatgagac gcgtctctcc aatctgcgac gtgagcgcgc
     6361 gacactgttc acctgtgcgc acgtcgcacc agatgatcga ggggcgcaac acttcatcgc
     6421 gttcgtcgag cagcgtcgcg ccgtgcatct gacccgatag accgacgcac tcgatctcgt
     6481 ccgcgcgcac ttcgccgcac gcgagcaccg cgcgcacggc ttcgcccgcc gcgcgccacc
     6541 agtcgcgcgc gtcctgttca gcccagcccg tctgcggcga aacgaacggc gcgtgctcgc
     6601 ttgtcgccgc agccgcgacg cgtcccgtct cgtcaacgac gacggcgcgc gtgccgcccg
     6661 tacctacatc tattcccagc agtttcatct cgactattta cttacgcaaa ggctcttgac
     6721 tcaagctctg gccgtgcgcc gttgcaacat ctgatcggcg atgacgccga tgaggatcac
     6781 gccgcccatc acggcgaagt tgagcgagga ggggatgccg agcaggttga cgagattctg
     6841 caagacttgc aggagcgcgg tgccgagcag gatgccgatg atcgaacctt cgccgccgcg
     6901 cagggagcag ccgccgagaa ccgccgccgc aatgccg
//
LOCUS       DASWNS010000598        11586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_231880, whole genome shotgun sequence.
ACCESSION   DASWNS010000598 DASWNS010000000
VERSION     DASWNS010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231880"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VGB76_20520"
     CDS             complement(<1..381)
                     /locus_tag="VGB76_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101320.1"
                     /translation="MRVLAVRTVSSSVLFFFFLLLFVLPAPARADGIVITSGFLHRPG
                     PAPSGPTFSFGNPAQGFAISNVGYGHGDGGGGWAICFPCTAGQTVSISGQFSGGSTLG
                     YGPATIGGVNYERVYYESGNLVFNA"
     gene            complement(550..1245)
                     /locus_tag="VGB76_20525"
     CDS             complement(550..1245)
                     /locus_tag="VGB76_20525"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY0101321.1"
                     /translation="MSKVIVTAIATLVAVSLLLLSGATLNHASGAHENLMQAEIHSDA
                     ETKRVEARAEESAPATAPFALSPSSQQSPLKLPLVNPKIVVAKSARQLSLYSDGRVVR
                     TYRIALGANPSDDKQRQGDRATPEGDFYVCVKNARSNFYLSLGLSYPNIEDAERGLRD
                     KLITRAERNAIVRAIQNKRRPPWDTALGGEIFIHGGGTEGDWTWGCVALANPDIKELF
                     DAVPVGTSVRIEH"
     gene            complement(1273..2946)
                     /locus_tag="VGB76_20530"
     CDS             complement(1273..2946)
                     /locus_tag="VGB76_20530"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEY0101322.1"
                     /translation="MRKVFEMKKLLRSVVLLVVLCGAFSPAASQSQAGAGRRAWITVL
                     STTDLHGNILPIDYYTNKPDARGLAKVATLIKAARRENPAGTLLLDSGDTIQGTPLQY
                     FHNKRNNTPPDPMMLTMSALKFDALAVGNHEYNFGLRVLEKARREASFPWLSANTYKR
                     GTNETYYQPYLVKELNGVRVGVIGLTTPGVPFWENRENFEGLEFREPLVEARKWVGVL
                     REKERVDLVVVAMHMGLEEDLRTGEVSPGQVTNENVAVRLAKGVAGIDLILMGHTHRE
                     VPSVVIGDTLLTQANSWGRHLARADVYLERDGRGRWRVAAKSARTIAVNERTETDEEI
                     AKLSAPYDLETQNWLARVIGESPQELTAEGSLFRDTAILDLVQRVQLDVGKADVSMVA
                     SFNPRARLPRGAVTVRDIAGLYIYENTLVVLEVTGAQLKDALEHSAKFFRAYEAGRTP
                     AELVDEKIPAYNFDIAEGVTYDLDLTKPHGARVQNLRFRGQPLNPAQKLRLATNNYRV
                     NGGGGYTMYKGAPELYRSSTEIRELIIDWVERERRVPAEPTNNWRLLTR"
     gene            complement(3066..4748)
                     /locus_tag="VGB76_20535"
     CDS             complement(3066..4748)
                     /locus_tag="VGB76_20535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0101323.1"
                     /translation="MERTLELTPEERAGGGDASHAATQGRGEHAGVLERGVALVLVLL
                     MLFGLALRVQGISELGFAEDEINKLEAARAYARGDITPNAEHPMLMKTLIFISLRASE
                     SWNATAGAARQISEETAVRLPNVLSGALTVFPLYLLTATFFGKRTGLLAALLWAFGVN
                     AITHNRIAKEDTLLVFFMLFAFYFYLRAKQTSGYEPRAKKRFYLASAVAFGLMLASKY
                     FPHYFGLNMLYHHFFAQREREPGEPRGRTPLIFYPLVILTFLIANPAVLLPQAWHYLT
                     AYSSEGLLTHHGYLMGDTLYTNQMSSTPFGTPFYFYLLFLAIKTPLPLLFALVAGLVA
                     CARRYKRPGEAFLLLMFILWIVPYSVAGAKWLRYALSLLPFVYMLAAVGIMWLLQRLD
                     APQRAGVERPGSFAPLRIVRAVVFLCFIALPAWVAYAAAPHYATYTNALGFGRAGYFF
                     PHDEFYDDGLREAINYVADHAPPGATIVHETPGVVRHYLQRFNRADLNSRVLSDPAFK
                     FVETRRPAYVILQRGRTYFENREKMSEARTRGRKVYEGFVNGLSAVEVYEVR"
     gene            complement(4773..5987)
                     /locus_tag="VGB76_20540"
     CDS             complement(4773..5987)
                     /locus_tag="VGB76_20540"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEY0101324.1"
                     /translation="MSKSTQEAMGGEARRSASGKFAPAGIIFAIGGTLLFAYYVWKAG
                     PQSIWANITQLGAGFIVVLAISAVRPLVRAASWTRCFEGDVRLRLRDALKAYVAGDAL
                     GSLTPFGMIVSEPAKAAFVRDRVPIVAAISALAVENLFYMLSVALFIFAGTAALLLSF
                     PLNSKLRFASYATLAVVVVFILIGYLMVRQRWRFLSGALAMLHARGIARRFVDARQER
                     FRAVEDRIYGFYERNRSRFLSILSLEACFHLAGVAEVYATLYFILETPPAFGELALAA
                     FVLESVNRVINVIFKFVPMRVGVDEAGTGLFTKVLKFGTTVGVTLAIVRKARVIVWTA
                     IGVAVLVRRGLSLRSMAREAQEAAALAATAAGSETGRSRAEEASAGIEHKDGGSSNNS
                     NVIDEEELIAPR"
     gene            6501..7694
                     /locus_tag="VGB76_20545"
     CDS             6501..7694
                     /locus_tag="VGB76_20545"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0101325.1"
                     /translation="MSKAATELLSGTPLTVYSEDENMFRASVREFAEGEIRPRREAMD
                     EHAKMDASIIKHLFDLGLMSIETPEEYGGTGASFFSAIIGVEELSRVDPSVGVLMDVQ
                     NTLVNNAIIRWGNAEQKKKYLAQLATDKIGAYALSEASSGSDAFAMQTKAVDAGDHYV
                     LNGQKLWITNGMEAEIFVVFANAKPEEGYRGVTAFIVEKGFEGISVGKKENKLGIRAS
                     STTEIIFDNCRVPKENLLGEFGKGYKVSIETLNEGRIGIGAQMIGLARGALEAALAYT
                     SERQQFGKAINQFQGVQFQLAELAVELEAARLLVYNAARLKDAGLPFVKEAAMAKLYS
                     SRAAETIASKAIELYGGYGFVKDYPVEKFWRDSKIGAIYEGTSNMQLQTIAKLMIDNR
                     QPKVQ"
     gene            7792..8109
                     /locus_tag="VGB76_20550"
     CDS             7792..8109
                     /locus_tag="VGB76_20550"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101326.1"
                     /translation="MSSRIEVFEQMLESDPENTLVLFGLAKEYEKSGRHMDVVATLTK
                     YLQLFNDEGNAYGMLAQAHEHLGNREAARAAYRRGIEVATAHGHPSMAEDYRMTLENE
                     YED"
     gene            8197..10437
                     /locus_tag="VGB76_20555"
     CDS             8197..10437
                     /locus_tag="VGB76_20555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0101327.1"
                     /translation="MQSFTTLRRASALVLALLLFAHTPLAGQTQPQTAPQDQDDLIRV
                     NTELLQATVTVLDKQGRFVEGLEREQFELKVDGKPQPISFFERVAAGSASEETLLAAA
                     RGKSPATTGEKSLSQGGLPDRGRSFFLFVDDLHMQVDSAMRARKTIHQFIEREIGQND
                     QAAVTSASGQIGFLQQLTDNKSVLRAAADRLKPLPQNTNDTQTPPMSEYLAHAIIDRH
                     DRDVLAVFVEALLRDMIPASSAENMVRTRAEQILRQSFARTRNTLASLSSLVRNAAAL
                     PGRKVVFFLSDGFLMRPSESSMNEQLRSLTDAAARSGVVIYTADARGLSVDPSLDAGS
                     PGLFDVRASRANAGELSAMQEPLRAIAEDTGGRALLNTNAIEAAIGKTIKETSAYYVV
                     AWQATGGEQKGGKFRRLEVSVKGRPELNVLAQRGFLQRGTDEQVASKAGATKTTMQQQ
                     GGKAKTPAEELRNAVGAVYPPTDLSTYLTVNYADTPKNGIIMTASLAVPFDALAFDSS
                     SGKNEGALDIEGHIYNDQGKSGSRFKERLSVSPTAATAAAAEPTPEQATGPGAGQTTY
                     GQHRSVIYSHQVNLAPGLYQVRVAARDARSGRIGGATQWVEIPNLKSGRLALSSLQLG
                     ERPAKDEAVYDVSNFLGQFSVDHRYTRASALRFLVFIYNAAQGAGGAPPDVVLQIQVF
                     RNDQPVITDELRKVTVEGQDLARIAYGAEVALESLVIGRYVLQVTVIDRVAKTSAAQR
                     VNFTVE"
     gene            10588..>11586
                     /locus_tag="VGB76_20560"
     CDS             10588..>11586
                     /locus_tag="VGB76_20560"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY0101328.1"
                     /translation="MATELKAREYHGLDREQMLGIYRTMYLSRRVDDKEIQLKGQNKI
                     FFQISGAGHEAVLTAAGLALKPAYDWFYPYYRDRALCLQLGMTPLEQLLSAVGAEADP
                     NSHGRQMPSHWGHKRLNIVSQSSPTGTQFLQAVGCAEAGYRSQLIEELKGRIENFQAD
                     EVVYVSTGDGTTSQGEFWEALNTASNLKLPVLFLVEDNGYAISVPVEVQTAGGDISRL
                     VAGFPDLYLQKCDGTDPVESFETLQRAVEYCRARKGPALVHAKVIRPYSHSLSDDEKL
                     YRPDEERTTDAERDPVKRFGALLIEEGFVTQEELQKLKDEVDAEVSAAADLALASPQ"
BASE COUNT         2332 a         3570 c         3609 g         2075 t
ORIGIN      
        1 cgcgttgaag accagattcc cggactcgta atagacgcgt tcatagttga ccccgccgat
       61 tgttgccgga ccatagccga gcgtcgaacc gccggagaac tgcccgctga tgctgacggt
      121 ctgaccggcg gtgcagggaa aacagatcgc ccacccgccg ccgccgtcgc catgcccata
      181 accgacgttt gagatggcga acccctgcgc aggattcccg aacgagaacg tgggaccgct
      241 cggagctggt cccggcctgt gcaggaagcc gctcgtgatg acgatcccgt cggcgcgcgc
      301 aggcgcggga agcacgaaga ggagcaggaa gaaaaagaag aggacggatg agctgacggt
      361 tctgacggcg aggacgcgca tggtgtgacc tttccttgag atgttagaga cgctggagac
      421 ggcgggaatt atccggcggc aactcccgag acaagttttt cgcggttcga ggcacagtct
      481 gtccgaggtt caaacgcttg tcaaggttat ttcggcttta tgcggaggcg caatgcgaac
      541 gcgtcgcagc tagtgttcga tgcggacgct tgtgcccacc ggcacggcgt cgaagagttc
      601 tttgatgtcc gggttggcga gcgcgacgca gccccaggtc cagtcgcctt cggtgccgcc
      661 gccgtgtatg aagatctcgc cgccaagcgc ggtgtcccac ggcggacgcc gcttgttctg
      721 tatcgcgcgc acaatggcat tgcgctctgc gcgcgtgatg agtttgtcgc gcaggccgcg
      781 ttcggcgtct tcaatgttcg gatagctcag gccgagcgag agataaaaat tgctgcgcgc
      841 gtttttgacg cagacgtaga agtcgccttc cggcgtcgcg cggtcgccct gcctctgttt
      901 atcgtcagac gggtttgcgc cgagcgcgat gcgataggtg cgtacgacgc gcccgtccga
      961 atagagagag agttgacgcg cgcttttagc gacgacgatt ttcggattga cgagcggaag
     1021 tttgagcggc gactgctgcg acgagggcga caacgcgaac ggagcggtcg caggcgcact
     1081 ctcctctgcg cgcgcctcga cccgtttcgt ctccgcgtct gaatgaattt cagcttgcat
     1141 cagattttcg tgcgcgccgc tcgcatgatt caaggttgcg cccgacagga gcaggagact
     1201 cactgcgacg agcgttgcta tcgcggtaac aataactttc gacatctttc agcctcgaac
     1261 gcaaaacgcg cttcaacgtg tcaacaaccg ccagttgttc gtcggctccg ccgggacgcg
     1321 ccgctcgcgt tcaacccaat caatgatcaa ctcgcgtatc tccgtgcttg agcgatagag
     1381 ttcgggcgcg cccttgtaca tcgtgtagcc gccgccgccg ttgacgcgat agttgttggt
     1441 ggcgagtcgc agtttctgcg cggggttgag cggctgcccg cgaaagcgca gattctggac
     1501 gcgcgcgccg tgcggtttcg tcagatcgag atcgtaggtg acgccctccg cgatgtcgaa
     1561 attgtaggcg ggaatttttt cgtccacgag ttcggcgggc gtgcgtcccg cttcataggc
     1621 gcgaaaaaat ttcgcggagt gttcgagcgc gtccttcaac tgcgcgcccg tcacttcgag
     1681 tacgacgagc gtgttctcgt aaatatagag tcccgcgatg tcgcgcaccg tcaccgcgcc
     1741 gcgaggcagg cgcgcacgcg ggttgaaact cgccaccatc gaaacgtccg ccttgcctac
     1801 gtccaactgc acgcgctgga ctaaatccag aatcgccgtg tcgcgaaaaa gcgatccctc
     1861 ggcggtcaac tcctgcggcg attcgccgat gacgcgcgcg agccagttct gcgtctccag
     1921 atcataaggc gcggagagtt ttgcgatctc ctcgtccgtc tccgtccgct cgttaacggc
     1981 gatggtgcgc gccgacttcg ccgcgacgcg ccaacgcccg cgcccgtccc tttccagata
     2041 cacgtccgcg cgcgccaagt gccgccccca ggagttcgcc tgcgtgagca gcgtgtcgcc
     2101 gatgacgacg gacggcacct cgcggtgcgt gtgtcccatc aggatgagat caatgcccgc
     2161 cacgcccttc gcaagtcgca ccgcgacatt ctcgttcgtc acctgaccgg ggctgacttc
     2221 gcccgtgcgc aagtcctctt ccagccccat gtgcatggcg acgacgacga gatcgacgcg
     2281 ctctttctcg cgcaggacgc cgacccactt gcgcgcttcc acgagcggct cacgaaactc
     2341 aagcccctcg aagttctccc tgttctccca gaagggcacg cccggcgtcg tcaaaccgat
     2401 gacgccgacg cgcacgccgt tgagttcctt gacgagatag ggctggtagt aagtctcgtt
     2461 tgtgccgcgc ttgtaagtgt tggcggagag ccacgggaaa ctcgcctcgc gccgcgcctt
     2521 ctccagcacg cggaggccga agttgtactc gtgattgcct acggcgagcg cgtcgaattt
     2581 gagcgcgctc atcgtgagca tcatcgggtc gggcggcgtg ttgttccgct tgttgtgaaa
     2641 atactggagc ggcgtgccct gaatcgtgtc gcccgaatcg aggagcagcg tgcctgcggg
     2701 gttctctctg cgcgccgctt tgatgagcgt cgccaccttc gccagcccgc gcgcgtccgg
     2761 cttgttcgtg tagtaatcaa tgggcaggat gttgccgtgc aagtccgtcg ttgacaggac
     2821 ggtgatccac gcgcgccgcc ctgctcccgc ctgcgactgc gaagccgcag gactaaatgc
     2881 cccacacagc accaccagca acacgacgct tcgcagcaac tttttcatct caaaaacttt
     2941 tctcattgct ccctgtttcc tgtgcctctc tgcgcctccg gcgtgagata gattttcgcc
     3001 ccggagacgc gcagacgcaa agaagaaaag accctcccgg ttaacttcat aaactccgac
     3061 cgcgcttatc taacttcgta gacttcgacc gcgctcaggc cgttgacgaa tccttcataa
     3121 actttccggc cacgcgtgcg cgcttccgac atcttctcac gattctcgaa ataggtgcgc
     3181 ccgcgttgga ggatgacgta ggcggggcgt cgcgtctcca cgaatttgaa ggcggggtcg
     3241 gagagcacgc gcgagttgag atcggcgcga ttgaaccttt gcaggtagtg gcgcacgacg
     3301 ccgggcgtct cgtgcacgat ggtcgcgccg ggcggcgcgt ggtctgcgac atagttgatc
     3361 gcttcgcgca ggccgtcgtc gtaaaactcg tcgtgcggga agaagtagcc cgcgcgcccg
     3421 aagcccaacg cgttcgtata agtcgcgtag tgcggcgcgg cggcgtaggc cacccacgcg
     3481 ggcagcgcga tgaagcagag gaagacgacg gcgcgcacga ttcgcagcgg cgcaaaactg
     3541 ccggggcgtt caacgcctgc gcgctgcggt gcgtcgagac gctgtaacag ccacatgatg
     3601 ccgaccgccg cgagcatgta gacgaacggc aggagcgaga gcgcgtagcg caaccacttc
     3661 gcgcccgcga cggagtaggg cacgatccag agaatgaaca tcaacaggag aaacgcttcg
     3721 cccggtcgct tgtagcggcg ggcgcaggcg acgagtccgg cgacgagtgc gaagagcagc
     3781 gggagcggcg tcttgatggc gaggaagagc aggtaaaaat agaagggcgt gccgaagggc
     3841 gtgctcgaca tctgattcgt gtagagcgtg tcgcccatca aatagccgtg atgcgtgagc
     3901 agtccctcgc tcgaataggc ggtcaggtag tgccacgcct gcggcagcag cacggcgggg
     3961 ttggcgatga gaaacgtgag gatgacgagg gggtagaaga tcaaaggcgt gcgcccgcgc
     4021 ggttcgccgg gttcgcgttc gcgctgcgcg aagaagtggt gatagagcat gttcagcccg
     4081 aagtagtggg ggaaatattt cgacgcgagc atcagcccga acgcgaccgc agaggctaag
     4141 taaaatctct tcttcgcacg cggttcgtag ccgctcgtct gtttggcgcg caggtaaaaa
     4201 tagaaggcga agagcatgaa gaagaccaga agcgtgtctt ctttggcgat gcggttgtgg
     4261 gtgatcgcgt tgacgccgaa cgcccagagg agcgcggcaa ggagtccggt gcgtttgccg
     4321 aagaaggtcg cggttaagag gtagagcgga aagaccgtga gcgcgccgga gagcacgttg
     4381 gggagacgca ccgccgtctc ttcgctgatc tgtcgcgccg cgcccgccgt cgcgttccag
     4441 ctttccgacg cccggagcga gatgaagatg agcgtcttca tcagcatcgg gtgttcggcg
     4501 ttcggcgtga tgtcgccgcg tgcataggcg cgcgcggctt cgagtttatt gatctcgtct
     4561 tcggcgaaac cgagttcgga gatgccctgc acgcgcaagg ccagcccgaa caacatgagg
     4621 aggacgagga cgagcgcgac gccgcgttcg agcacgcccg catgctcgcc gcgcccctgc
     4681 gttgcggcgt gcgaagcgtc gccgccgccc gccctctctt cgggagttaa ttcgagagtt
     4741 cgttccaact aaaactccag acacgaaacg ctctagcgag gcgcgatgag ttcctcttcg
     4801 tcgatcacat tgctgttgtt actgctgccg ccgtccttgt gctcgatgcc cgccgacgcc
     4861 tcttctgcgc ggctgcgccc ggtttcgctc ccggcggcgg tggcagcgag ggcggctgct
     4921 tcctgcgctt cgcgcgccat gctgcgcaga gacagcccgc gcctgacgag gactgcgacg
     4981 ccgatggccg tccagacgat gacgcgcgct ttgcgcacga tggcgagcgt gacgcctacg
     5041 gtcgtcccga atttcaagac tttggtaaac agccccgtcc cggcttcgtc cacgccgaca
     5101 cgcatcggca cgaatttgaa gatcacgttg atcacgcggt tgacggattc gaggacgaag
     5161 gccgcgagcg cgagttcgcc aaaagcggga ggagtttcga gtatgaagta gagcgtggcg
     5221 tagacctcgg ccacgcccgc gaggtgaaag caggcttcga gcgagaggat cgagagaaag
     5281 cgcgaacggt tgcgttcgta aaagccgtag atgcggtctt cgacggcgcg gaaacgctcc
     5341 tgtcgcgcgt ctacgaaacg gcgcgcgatg ccgcgtgcgt gcagcatggc gagcgcgccg
     5401 ctcaaaaatc gccaacgctg ccgcaccatc aaataaccga tgaggatgaa gaccacaacc
     5461 accgcgagcg tcgcgtagct ggcgaagcgg agtttcgagt tcagtgggaa actgaggagc
     5521 aaggccgccg tgcccgcgaa gatgaagagc gcgaccgaga gcatgtagaa gagattttcg
     5581 acggcgagcg cggagatggc tgcgacgatg ggcacgcggt cgcgcacgaa ggccgccttc
     5641 gccggttcgc tcacgatcat gccgaagggc gtcaaacttc cgagcgcgtc gcccgcgacg
     5701 taggctttga gcgcgtcgcg caggcgcagg cgcacgtcac cttcgaagca gcgcgtccag
     5761 gaggcggcgc gcacgagcgg gcgcacggcg gagatggcga ggacgacgat aaagcccgcg
     5821 ccgagttgcg tgatgttcgc ccagatgctc tgcggtccgg ccttccacac gtagtaggcg
     5881 aagagcagcg tgccgccgat ggcgaagatg atgcccgccg gggcaaattt gcccgacgcg
     5941 ctccgccgcg cttcgccccc catcgcctcc tgcgtgctct tgctcattcc atcatgccct
     6001 gccggaagag atcgccgctc ccgcccgaag ttcgtcgtca ataataagcg tctgcaagat
     6061 acgttattgc gtcgccagtc tcaactgccg cactcacacg ccgccgcgcc cgacgcgctt
     6121 ctcccacaca gcaactcccg cgccaccctc cgcccgcgcg ccgccaacgc cgcgaaacac
     6181 gcgccgcagc ccgcccgcca caaaccgcgc ccgcgcgccc gtgtcgtaaa aatccgacag
     6241 gagatcagga gcggtgacaa gtgacgagtg acaggcgacg agattaagaa agtaaatcgt
     6301 taatcgtgaa tagagaaaac tgctttagtc tatttacgag tcacgattta cgattcacgg
     6361 ctttttctcg tcacttggca ctcgtcactc gtcactgctt tcgagggttg accggaggcg
     6421 cattgatggg cgatgcttac gtgtacaaac gtatcagccc taacattttg aatcttaacc
     6481 aagaaggtct gtaaagagcg atgagcaaag cagcaaccga acttttgagc ggcacgcctt
     6541 taaccgtcta ttccgaggac gaaaacatgt ttcgcgccag cgtgcgcgag tttgccgaag
     6601 gcgagattcg cccgcgccgc gaggcgatgg acgagcacgc caagatggac gcttcgatca
     6661 tcaagcacct cttcgacctc ggtttgatga gcatcgaaac acccgaagaa tatggcggca
     6721 cgggcgcgtc cttcttctcg gccatcatcg gcgtcgaaga actctcgcgc gtcgatcctt
     6781 cggtcggcgt cctgatggac gtgcagaaca cgctcgtcaa caacgccatc atccgctggg
     6841 gcaacgccga acagaagaag aagtatctcg ctcaactcgc caccgacaag atcggcgcat
     6901 atgctctcag cgaagccagt tccggctccg acgccttcgc catgcagacc aaggcggttg
     6961 acgcgggcga ccactacgtt ttgaacggtc agaaactctg gatcacgaac ggcatggaag
     7021 ccgaaatctt cgtcgtcttc gccaacgcca aacccgaaga aggttacagg ggcgttaccg
     7081 ccttcatcgt cgagaagggt tttgaaggca tcagcgtggg caagaaggag aacaaactcg
     7141 gcatccgcgc ctcttccacc accgagatca tcttcgacaa ctgccgcgtg ccgaaagaga
     7201 atctgctcgg cgaattcggc aaaggttaca aagtctccat cgagacgctg aacgaagggc
     7261 gcatcggcat cggcgcacag atgatcggcc tcgcacgcgg cgcgctcgaa gccgcgctcg
     7321 cctacacgtc ggagcggcaa cagttcggca aggccatcaa tcaatttcag ggcgtgcagt
     7381 tccaactcgc cgaactcgcc gtcgaactcg aagccgcgcg cctgctggtc tataacgccg
     7441 cgcgtttgaa agacgccggg ctgcccttcg tcaaagaggc cgcgatggcg aaactctatt
     7501 cgtcgcgcgc cgccgaaacc atcgcctcca aagccatcga actctacggc ggctacggct
     7561 tcgtcaaaga ctacccggtc gaaaaattct ggcgcgactc caagatcggc gcgatctacg
     7621 aaggcacgtc caacatgcaa ctgcaaacca tcgccaagct gatgatcgac aaccggcagc
     7681 cgaaagtaca atgagaaagc agtgacaagt gccgcgtgac gtgtgacaag ttaaaacaat
     7741 aaaacttgtc ttgcttcctg ctcgtcactg gtcacctgtc acttaataat tatgtcttca
     7801 cgcatagaag tcttcgagca gatgcttgag agcgacccgg aaaacacgtt ggtcttgttc
     7861 gggctggcga aggagtatga gaagtccggg cggcacatgg acgtggtggc gacgctcacg
     7921 aagtatctgc aacttttcaa cgacgagggg aacgcttacg gtatgctcgc gcaggcgcac
     7981 gagcatctcg gcaaccgcga ggcggcacgc gcagcctacc ggcgcggcat cgaagtcgcc
     8041 acggcgcacg gccacccttc gatggcggaa gactaccgga tgacgcttga gaacgagtac
     8101 gaagattaga cgacggtgcg ccgtgcgctg cccggagcag tttcattcgc cgtagcagtt
     8161 tcattcgctt caggtctttt gtcggggtgt ccttgcatgc aatccttcac cactttacgc
     8221 cgcgcatcag ccctcgtctt agccctcctt ctcttcgcgc acacgcccct cgccgggcag
     8281 actcagccgc agactgcgcc gcaggatcag gacgatttga tcagggtcaa cacggaactg
     8341 ttgcaggcga ccgtcacggt gctcgataag caggggcgtt tcgtagaggg cttggagcgc
     8401 gaacaattcg agttgaaagt tgacggcaag ccgcagccga tctctttctt cgagcgggtg
     8461 gcggcgggca gcgcgagcga ggagacgctg ctggcggcgg cgcgcggcaa gtctcctgcg
     8521 acgacgggag aaaagagttt gtcgcagggc gggctgccgg atcgcggtcg ctcgttcttc
     8581 ctgttcgtgg acgacctgca catgcaggtt gacagcgcga tgcgcgcacg caagacgatt
     8641 caccagttta tcgaacggga gataggtcaa aacgatcagg ccgccgttac ctctgcgagc
     8701 ggccagatcg gttttctaca gcagttgacc gacaacaaga gcgtcctgcg cgccgccgcc
     8761 gaccgtctca agccgctgcc gcagaacacg aacgacacgc agactccgcc catgtcggaa
     8821 tatctggcgc acgccattat cgatagacac gaccgcgatg tgctggccgt tttcgtcgag
     8881 gcgttgctca gggacatgat cccggcgagt tccgccgaaa acatggtgag gacgcgcgcc
     8941 gagcagattc tcaggcagtc gtttgcgcgc acgaggaaca cgctcgcctc gctctcttcg
     9001 ctcgtgcgca acgccgccgc gctgccgggg cgcaaggtcg tcttctttct gtccgacggt
     9061 tttctgatgc gcccgagcga gtcgagcatg aacgaacaac tgcgctcgct cacggacgcg
     9121 gcggcgcgca gcggcgtggt gatctatacg gctgacgcgc gcggcctcag cgtcgatccg
     9181 tcgctcgacg cgggcagtcc cggcctcttc gacgtgcgcg cttcgcgtgc caacgccgga
     9241 gagttgtccg ccatgcagga gcctctgcgc gcgattgcgg aagacacggg cgggcgcgcg
     9301 ctgctcaaca ccaacgccat cgaagcggcc atcggcaaga ccatcaagga gacttccgcc
     9361 tattacgtgg tggcgtggca ggcgacgggc ggcgagcaga agggcggaaa gtttcgccgc
     9421 ctggaagtca gcgtcaaggg acgcccggag ttgaacgtgc tcgcgcagcg cggctttctc
     9481 cagaggggca ccgacgaaca ggtcgccagc aaggcgggcg cgaccaagac gacgatgcag
     9541 cagcagggcg gcaaggcgaa gacgcccgcc gaagagttgc gcaacgccgt cggagccgtc
     9601 tatcccccga ccgacctctc gacgtacctg accgtcaact acgcggacac gccgaaaaac
     9661 ggcatcatca tgacggcctc gctcgccgtc cctttcgacg ccctcgcgtt cgactcttcc
     9721 agcgggaaga acgaaggcgc gctcgacatc gaaggccaca tctataacga tcagggcaag
     9781 tccggctcgc gcttcaaaga acgcctgagc gtctcgccca ccgccgcgac cgctgccgcc
     9841 gcagagccga cgccggagca ggcgaccgga ccgggcgcgg ggcagacaac ttacgggcag
     9901 catcgcagcg ttatatacag ccatcaggtg aacctcgccc ccggcctcta tcaggtgcgc
     9961 gtcgcggcgc gcgatgcgcg gagcgggcgc atcggcggcg caacgcagtg ggtcgagatt
    10021 cccaatctca aatccggacg cctcgccctg agcagcctcc aactcggcga acgcccggct
    10081 aaggacgaag cggtctacga cgtgtcgaac tttctcgggc agttcagcgt cgatcaccgc
    10141 tacacacgcg cctcggcctt gcgcttcctc gtctttatct ataacgcggc gcagggcgcg
    10201 gggggcgcgc cgcccgacgt ggtgctccag atacaggttt ttcgcaacga ccagccggtg
    10261 atcacggatg agttgcgcaa ggtgacggtt gaaggacaag accttgcgcg gatcgcttac
    10321 ggggcggaag tcgcgcttga gtcgctcgtg attggccgtt acgtgctaca agtgacggtg
    10381 attgaccgcg tggcgaagac gagcgcggcg cagcgggtta atttcacagt cgagtaacag
    10441 tcggagtagg cgtcaggggc gcgggcgcgt cacaattcat cagggcggcg ttgcgcgtcg
    10501 ccggaaacga aaggccgcga cttgcaatga ggccgcgcga aggtttatta aatttcgatt
    10561 atccaagaag tcaggagtag taacagcatg gcgaccgaat tgaaggcgcg tgaatatcat
    10621 ggactcgacc gcgagcagat gctcggcatc tatcgcacga tgtacctgtc gcggcgcgtg
    10681 gacgataaag agattcaact caaagggcag aacaaaatct ttttccagat cagcggcgcg
    10741 ggacacgaag ccgtgctcac ggcagcgggg ctggccttga aacccgctta cgattggttc
    10801 tacccctact accgcgaccg cgcgctctgc ctccaactcg gcatgacgcc cctcgaacaa
    10861 ctgctctcag ctgtcggcgc ggaggccgac cccaactcgc acgggcggca gatgccttca
    10921 cactgggggc acaagcgact caacatcgtc tcgcagtcgt cgccgacggg cacgcagttt
    10981 ttgcaggcgg tcggctgcgc cgaggccggt tatcgctccc agttgatcga ggagttgaaa
    11041 gggcgcatcg agaattttca ggccgatgaa gtcgtctatg tttcgaccgg cgacggcacg
    11101 acgagtcagg gcgaattctg ggaagctctc aacacggcga gcaacctgaa actccccgtg
    11161 ctctttctcg tggaagacaa cggctacgcc atcagcgtgc ccgtcgaagt gcagacggcg
    11221 ggcggcgaca tctcgcgcct cgtcgcgggc ttccctgatc tctacttgca gaagtgcgac
    11281 ggcaccgatc cggtcgaatc ttttgagacg ctccaacgcg ccgtcgagta ttgccgcgca
    11341 cgcaaaggtc ccgcgctcgt ccacgccaaa gtcatacgcc cctactcgca ttcgctgtcc
    11401 gacgacgaga aactctatcg ccccgacgaa gagcgcacga ccgatgccga acgcgatccc
    11461 gtcaaacgct tcggcgcgct ccttatcgaa gagggtttcg tgacgcagga ggagttgcaa
    11521 aagctcaagg acgaagttga cgccgaagtg agcgccgccg ccgacctcgc gctcgccagc
    11581 ccgcag
//
LOCUS       DASWNS010000599         4966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_439183, whole genome shotgun sequence.
ACCESSION   DASWNS010000599 DASWNS010000000
VERSION     DASWNS010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439183"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGB76_20565"
     CDS             complement(<1..435)
                     /locus_tag="VGB76_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101329.1"
                     /translation="MKIKTLFFILFISLTAATDSAAQTDPSVWAVDFVKTKEGRQPDY
                     LQFVEENWARARAFMKQKGIAHSYRVLSVPVSRQAEWDVLLVTEYAGRAGYERREDVF
                     KEYQASAAPGTPQRREAGNKIDARDISTIKFSHVFHQPVSSET"
     gene            complement(862..1026)
                     /locus_tag="VGB76_20570"
     CDS             complement(862..1026)
                     /locus_tag="VGB76_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101330.1"
                     /translation="MALVLLSLGEREEARRRVEKTHERIASKPALAHLLRTDPMYESL
                     RADPALRSLI"
     gene            1160..1423
                     /locus_tag="VGB76_20575"
     CDS             1160..1423
                     /locus_tag="VGB76_20575"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY0101331.1"
                     /translation="MDERIQKKEIVCRLASRMKTDEATAALWVDAILDTLYESFSHGQ
                     SVTLPGFGNFYVRPHREHWTFKFNPSQKLRALFGWSATYKGSR"
     gene            2062..3075
                     /locus_tag="VGB76_20580"
     CDS             2062..3075
                     /locus_tag="VGB76_20580"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HEY0101332.1"
                     /translation="MERGLNNSTDRVHTRAYVRLDLIHREISAGRYPNLRSLAELLER
                     NERTVKRDLRVLRDQMGAPIIFDRERGGFRYQQPGWELPPVNFNEGELLAFFTAEQAL
                     RATGHTPEAILLRAALAKLAAFLPPEISVNAATLGEALTFQQLPHVSVEPSTLHTLAR
                     AAATRRTISFDYHSQHRNEDNHRQADVLLLHNFAGDWYAICYDHLRREMRDFHAGRIQ
                     KLTLTSQYFDPPANWNADDYLRRGFHMMRGGRRTTVYIVFDPYQARWMKERIAFHPDE
                     KREDLPDGGLRLSFPIGRNGLEAVARFCLAYAGHCRAERPATLREMIRERLTQALEDH
                     KED"
     gene            3077..3763
                     /gene="cas6"
                     /locus_tag="VGB76_20585"
     CDS             3077..3763
                     /gene="cas6"
                     /locus_tag="VGB76_20585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein
                     Cas6/Cmx6"
                     /protein_id="HEY0101333.1"
                     /translation="MSTNAQHNITPASAINADTHPSVIVHFPVQGRQLPADHGYALYS
                     AITRQLPALHGASWLGLELLSGVPWREGIIVLPTRGAHLRLRIPADRYGHVLPLAGRR
                     LDISGHPISLGIPTARPLTPAPSLYARIVTIKKFTEPEPFLAAARRQLDALGLIATLE
                     LPTDEQGRPRRRIITIQGKHVVGFSLAAHGLNDEDSLHLQTSGIGGRRAMGCGLFNPI
                     ASNETLKEMK"
     gene            3763..>4966
                     /locus_tag="VGB76_20590"
     CDS             3763..>4966
                     /locus_tag="VGB76_20590"
                     /inference="COORDINATES: protein motif:HMM:NF037707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0101334.1"
                     /translation="MKRLLAKSYDREKHGNSPPDYALLTQHSRDVANACDALAIAVGR
                     TALHNAGLDLAEFERFQLTLRANGWIQDLGKANSHFQQMVSGDQQIRQLVRHETISGM
                     LMLLDERFRKWLSPLSETILASVWGAMGHHRKFDESITPEQTTALTVHVAHEDFSQIL
                     ADMNASLGLSAPPQFERDLVIARDGRETCDLQAREAIRDLQEDFMDREAEFADEGERR
                     MLALVKAFGIAADVAASAVAAKGRWAQNYSLTDYVAESLAIGLNPTDLSRLINTWAWD
                     RSNCEGASRDETLLPPTFIVRDFQNEVAESTSFLTLAQAGCGSGKSLAAYMWAREWCQ
                     KFERDARRNFRLYFCLPTTGTTTEHFKDYALESGIDASLISLTHSRSSVELKAMAETA
                     PQEEASEDV"
BASE COUNT         1107 a         1483 c         1357 g         1019 t
ORIGIN      
        1 cgtctcggat gaaaccggct ggtgaaagac gtggctgaat ttaatcgtcg agatgtctct
       61 ggcgtctatc ttgttaccgg cttctcttct ctgcggcgtg cccggcgcgg ctgatgcttg
      121 atattctttg aagacatcct cgcgccgttc gtagcccgct cgccccgcgt attcggtcac
      181 gagcaatacg tcccactccg cctgtcggct gaccggcaca gacagcacgc gataggagtg
      241 cgcgatcccc ttctgcttca taaaggcacg cgcacgcgcc cagttctctt caacgaactg
      301 tagatagtcc ggttgacgcc cctccttggt tttgacgaaa tctaccgccc acacggaagg
      361 atcggtctgc gcggcggaat ccgtcgcggc tgtcaatgaa atgaacagga tgaagaagag
      421 agttttgatt ttcatgtttg ctcctcagga tttctactaa tctcaaacgg ggaatgccgg
      481 gtcggcgtct aacgacgacg ttaggataaa cagagtgcgg gggtgcggtg gagggaaaat
      541 ctgcgagcgg tcgggcggta aagttcatcc ggcagatttg ccttactttc agccgtttta
      601 cgcttttgac cagaagggca actcccgatg agcggtcgag gggcggcagg aatattagcc
      661 ctgttatagg agtgggaggc catgtagcag gcgggagccg tagagttcag ccccggcggc
      721 tttgtcgctt tagtcgcagc gcaggcaccg gataagatgc gcgcgtttga tcttccgggg
      781 cagggtcgcg ggcatgcgag gtcttcaggc gacttcagaa catttcacgt cgcttgagga
      841 cttctcagcc tcacgccgcg cctaaataag actccgcaac gcggggtctg cgcggaggct
      901 ttcgtacatc gggtcggttc tgagcaggtg ggcgagcgcg ggcttgctag cgatcctttc
      961 gtgagtcttc tccacccggc ggcgtgcctc ctcccgctcg ccgagactga gcagaacgag
     1021 tgccacgtcc tgcggcgact tgccgccccc gacaggaggc cgatcatgcg gcgcgcttca
     1081 cgctttcgcc ccgcgaccgc gtaggcatag cccagcgccg aggcgacgcg caggtcgtat
     1141 caccgggagc cataaaatta tggatgaacg tattcagaag aaagaaatag tctgccgcct
     1201 tgcgtctcga atgaagaccg acgaagcaac agccgcgctc tgggtagatg ctatcctaga
     1261 cactctgtat gaaagtttca gccacggcca gagtgtgacg ctgcccggct tcggtaattt
     1321 ttatgtgcgc ccccaccgtg aacactggac attcaagttc aatccttcgc aaaaattgcg
     1381 cgcgctcttc ggttggtcgg ccacttacaa aggtagcagg tgaacgcgaa tatgtagcaa
     1441 agcaaccctc tgaaaatagc atgtgatggc cgaagcaatg acacactcac ccttgaacaa
     1501 cctgagccgc cgctcgatac gcgcactggc gggagagagt tacttcgcgc ggggagagac
     1561 ctacttcaac gaggggcgcg tgcgcggcct gacggagtac aaaggcacag tgagggcgaa
     1621 ggtcgcaggg acggaggatt atcgcgtcaa gctgtggaca gagggagata acatcggcta
     1681 ctcatgtagt tgtcctctcg gagacgacgg agagttttgc aaacattgtg tcgccgtcgg
     1741 tctggcatgg attgaagaga gtggaggaaa gagcaagagc ggcgccgcag cccgaaagtc
     1801 cacggctaca atgggctttg cccgacaatt caatatcgac attctatgtt gcgcgcgacg
     1861 aacagcgtac cacctgcatg ttgtggtcaa accttaattg tcgggcaaag ccaagacagc
     1921 tagtgggttg gatgagccga gtttttgatt ccaatgatgc cgaaaggcga ttttgatttt
     1981 tagcctgcta tttgaagaga agagatttta ttctatcaac aggacacaag ccgtccccct
     2041 caccgaatta taatcatcac tatggagaga ggcctcaaca actccacaga ccgcgtccat
     2101 acacgagcat atgttcgcct cgatctaatt cacagggaga tcagcgcagg ccgatatcca
     2161 aatttgcgct cgctcgccga gctgctggaa cgcaacgagc gcaccgtcaa acgcgatctg
     2221 cgcgtcctgc gcgaccagat gggcgcgccg atcatctttg accgcgagcg gggcggcttt
     2281 cgttatcaac aaccgggatg ggagttgccg cctgttaact ttaacgaagg agaacttctc
     2341 gccttcttca ccgccgaaca agccctacgc gccacaggcc acacgcccga agccatcctg
     2401 ctgcgcgccg cccttgccaa actcgcggct tttttgcctc ctgaaatcag cgtcaacgcg
     2461 gccacgctcg gcgaagcgtt gactttccag caacttccgc acgtcagcgt cgagccgtcc
     2521 acgctgcaca cacttgcccg tgccgccgcg acgcgtcgca cgatttcgtt tgactaccac
     2581 tcgcaacatc gcaacgaaga caaccatcgc caggctgatg ttcttctgct tcataacttc
     2641 gccggtgatt ggtacgcgat ctgttacgac cacctgcgcc gggagatgcg cgactttcac
     2701 gccgggcgta ttcagaaatt gacgctcacc tcgcaatact tcgaccctcc cgccaactgg
     2761 aacgccgacg attacctgcg tcgcggtttt cacatgatgc gcggcgggcg gcgcaccacc
     2821 gtctacatcg tcttcgatcc ttatcaagcg cgctggatga aagagcgcat cgcctttcat
     2881 cccgacgaga aacgcgaaga cctgcccgac ggcggactgc gtctctcctt ccccatcggg
     2941 cgcaacggcc tcgaagcggt tgcacgcttc tgcctcgctt acgccgggca ctgccgcgcc
     3001 gaacgccccg ccactctgcg cgaaatgatc cgcgaacgat taacacaggc acttgaagac
     3061 cacaaagagg actaatatga gcaccaacgc ccagcacaac atcacaccgg catccgcgat
     3121 taacgctgac acacacccat ccgtcatcgt tcactttccc gtgcaggggc gacaactccc
     3181 cgccgaccac ggctacgcgc tctactctgc catcacacga cagttgccag ccctgcacgg
     3241 cgcatcgtgg ctaggactgg aactcctcag cggtgttccc tggcgtgaag gtatcatcgt
     3301 gcttccaact cgcggcgcac atttacgcct ccgcattccc gccgaccgct acggccacgt
     3361 cctccccctc gccggacgcc gcctcgacat ctccggccac cccatcagcc tcggcatccc
     3421 caccgcacgc ccgctcacgc ccgccccttc gctctacgcg cgcatcgtta cgatcaagaa
     3481 attcaccgaa cccgaaccat tcctcgccgc cgcccgccgc caactcgacg cactcggcct
     3541 catagccacc ctcgaactcc ccacagacga acaaggccgc ccccgccgcc gcatcatcac
     3601 cattcaaggc aaacatgtcg tcggcttctc cctcgccgca cacggcttga acgacgagga
     3661 ttcactccat ctccaaacct ccggcatcgg cgggcggcga gcgatgggct gtgggctttt
     3721 caatccaatt gcaagcaacg agactttgaa ggagatgaag tagtgaaacg attgcttgct
     3781 aaatcttacg accgcgagaa gcatgggaac tcgccgcctg attacgcgct cttgacgcag
     3841 cacagtcgcg acgtagcgaa tgcctgcgat gcgttggcaa tcgcagtcgg tcgcaccgcc
     3901 ttgcacaatg caggactcga tctggctgag ttcgagagat tccaattgac cttgcgcgcc
     3961 aatggttgga ttcaggattt aggaaaggcg aacagtcact ttcagcagat ggtgagcggc
     4021 gaccagcaaa tcaggcaact agttaggcac gaaactattt cgggcatgtt gatgctgctt
     4081 gatgagcgtt ttcgcaagtg gttatctcct ctttcagaga caattctcgc ctctgtgtgg
     4141 ggagcgatgg gacatcatcg caagtttgat gaaagcatca cacctgaaca aacgacggct
     4201 ttaacggttc acgtcgcgca tgaagatttc agtcaaattc tcgctgacat gaacgcaagt
     4261 ttagggcttt ctgctccacc gcaattcgag cgtgatctag tgattgcgcg agatggtagg
     4321 gaaacttgtg atctacaggc gcgtgaagcg attcgagatt tgcaagagga tttcatggat
     4381 cgagaagcgg agtttgccga cgagggtgaa cgcagaatgc ttgcgctcgt caaagcgttc
     4441 ggcatcgccg ctgatgttgc agccagcgca gttgccgcga aaggacggtg ggcacaaaac
     4501 tattcactca ccgattatgt cgccgagagt ctggcgattg gcttgaaccc aaccgacctc
     4561 tcgcggctca tcaacacatg ggcatgggat cgctcaaact gtgaaggcgc gagccgtgat
     4621 gaaactttgc tcccacctac attcatcgtg cgcgattttc aaaacgaagt tgcggaatca
     4681 acttcatttc tgactctcgc tcaggctggc tgcggatcgg gcaaaagcct cgccgcgtac
     4741 atgtgggcac gcgagtggtg tcaaaaattt gagcgggacg cacgccgaaa cttccgcctc
     4801 tatttttgtt tgccaacaac gggcacaacg acagaacact tcaaagatta cgcacttgaa
     4861 tcggggattg atgcaagcct aataagcctg acgcactcgc gctccagcgt tgagctgaag
     4921 gcgatggcag aaactgcacc gcaggaagaa gcgagcgaag atgtcg
//
LOCUS       DASWNS010000600         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_491629, whole genome shotgun sequence.
ACCESSION   DASWNS010000600 DASWNS010000000
VERSION     DASWNS010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491629"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2886)
                     /locus_tag="VGB76_20595"
     CDS             complement(<1..2886)
                     /locus_tag="VGB76_20595"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEY0101335.1"
                     /translation="MVREDIPGHQNLAAYVVQNLQYVEADKKVALATQRVTQWQDVHN
                     DEVANETSISDEPTFNISGWKSSYTGEAIPEAEMREWLDATVERVLARKPESILEIGC
                     GTGLLLFRLAPHCTKYWGRDFSPAALDYVRQHLKLLDGGAAHVQLAQREATDFEGIEP
                     KSFDGVIINSVVQYFPGVDYFVRVLQGAIRSVREGGFIIVGDVRNLPLLEAFHTSVQL
                     EQADASLSIAQLRQQVQKALVEEPELVIDPAFFIALKQRLPEINHVEINLKRGHSQNE
                     LNRFRYDVILHVGATTQPEVNLKWVDWDKQGMSPDSVRQLLRETEPEFFGITNVPNAR
                     VLDAVQAGVLLSALNEAATVGELRKALRKEAGTAADPEDLWALADELPYEVDISWTGT
                     GADGRYDVLFTRRAATESGATNRAARVAYPLASEMTTPRRLSSYTNDPLQDTFARELI
                     PDVLNYLKEELPDYMIPTAFVMLEALPLLPSGKIDRRALPAPDWSTDELEAARAVPRN
                     AIEEILSGIWREVLGGGEVDIHDNFFELGGHSLLATQVMSRVRKTFKVEVPLRRLFEF
                     PTVATLAECIESASRADEYEVHVPPIVPVERGGRLPLSFAQQRLWFLHQLEPDSPAYN
                     VPAAVRLTGQLNVKALEQSFNEIIRRHETLRTTFATDQAGPVQIIAPVQKLTLAVLNI
                     SGLPETERELEAGRIAADEARLPFDLSQGPVLRVKLLRLDEEEHIVLLTMHHIVSDGW
                     SVGVLVGEVGALYDAYAAGKSSPLPELKIQYADFAVWQRQWLNDEILDLQLSYWKKRL
                     AGAPPSLNLPTDHPRPHIQSFQGATQAFAIPATTTEALRALSRREGVTLYMTLMAAFQ
                     TLMYRYTAQTDIVVGSNIANRNRMETEATIGFFSNMLVMRTDLSGDPTFQELLVRVRE
                     TALGAYAHQDVPFEKLVEALQPQREPGRQPMFQIVFSLQNAPMPALQL"
BASE COUNT          570 a          872 c          864 g          606 t
ORIGIN      
        1 aagctggagc gcgggcatcg gagcgttctg tagtgaaaag acgatctgga acatcggctg
       61 gcgtcccggc tcgcgttgcg gttgcaacgc ctcgaccagc ttttcgaatg gcacatcctg
      121 gtgtgcgtat gcgccgagcg cggtttcacg cacgcgaacc aacagttcct gaaaggtcgg
      181 gtcgcctgag aggtctgtgc gcatgaccag catgttggag aaaaatccga tagtcgcttc
      241 cgtctccatg cggttccggt tagcgatatt cgagccgacg acaatatccg tctgcgccgt
      301 gtagcgatac atcaaggtct ggaacgcagc catcagcgtc atatacagcg tgacgccttc
      361 gcggcggctg agcgcacgga gagcctcggt agtggtggcg ggaatggcga aggcctgagt
      421 tgcgccctga aagctctgga tgtgcggtcg cgggtggtcg gtcggcaggt tcagagaggg
      481 cggagcaccg gccagacgct tcttccagta gctcaactgt agatcgagga tttcgtcgtt
      541 cagccattga cgctgccaga cggcgaagtc ggcatattga atctttaatt ccgggagcgg
      601 agaggatttg cccgccgcgt aggcgtcgta gagcgcgcct acttcgccga ccagcacgcc
      661 gaccgaccat ccatcggaaa cgatgtgatg catcgtcagc aagacgatgt gctcttcctc
      721 atccagacgc agcagcttga cgcgcagaac cggcccctgc gaaaggtcga aggggagacg
      781 cgcctcatcc gccgcgatcc gcccggcctc aagctctcgc tccgtttcgg gcaacccgct
      841 gatattcaga accgccagcg tcaatttttg taccggggcg atgatctgga ctggacccgc
      901 ctgatcggtg gcgaacgtcg tgcgcagagt ttcatggcga cgaatgatct cgttgaagct
      961 ctgttcgagc gccttcacgt tgagttgccc ggtcaggcgc acggcggcgg gcacgttata
     1021 ggcgggcgaa tccggctcca gttgatggag gaaccataac cgctgttggg caaaagagag
     1081 cggcaggcgt ccgcccctct cgaccggaac gatgggcggc acatgcactt catactcatc
     1141 ggcgcgactg gccgactcga tacactcggc cagcgtggcg accgtcggga attcaaagag
     1201 acgccgcagc ggcacttcca ccttgaaggt tttacgcacg cgggacatga cctgcgtggc
     1261 gagcagagag tgtccgccga gttcgaagaa gttgtcgtga atgtctacct ctccgccgcc
     1321 gagaacctct ctccatattc cgctcaggat ctcttcaatc gcgttgcgcg gaacggcgcg
     1381 cgccgcttcc agttcgtctg tgctccagtc cggcgcaggc aaagcgcgcc ggtctatttt
     1441 gccgctcggc agcagcggca acgcttcgag catgacgaag gctgtcggga tcatgtaatc
     1501 cggcaactct tctttcaggt agttgagcac atcgggaatc aactcgcgtg cgaaggtgtc
     1561 ctggagcgga tcattcgtat aagaactaag gcggcgcggc gtggtcatct cagaggccag
     1621 cggataagcg acgcgtgcgg cgcggttcgt cgccccgctt tcggttgccg ctctgcgtgt
     1681 aaacagcacg tcgtagcgtc cgtccgcgcc cgtgccggtc caactgatgt caacctcata
     1741 gggcaactcg tcggccaaag cccacaggtc ttccgggtct gcggcggtgc ccgcctcttt
     1801 ccgtagagcc ttccgcagtt cgccgacggt ggcggcttcg ttcaaagcgg acagcagcac
     1861 gcccgcctgc accgcatcca acacgcgcgc attgggaacg ttcgtgatcc cgaaaaactc
     1921 cggttcggtt tcccgcagca gttgccgcac ggaatccgga gacatgccct gtttgtccca
     1981 atcaacccat ttcaaattga cttccggctg cgtcgtggct cccacgtgca gaattacgtc
     2041 gtaacggaag cggttcaact cgttctgaga gtggccgcgc ttgagattga tctcgacatg
     2101 gttgatctcc ggcagccgtt gtttcagagc aatgaagaag gcagggtcga tgaccagttc
     2161 gggttcttcg accagagcct tctgcacctg ctgtcgcaac tgtgcgatgg agagcgaagc
     2221 gtccgcctgt tctaattgaa ccgaagtgtg aaacgcttcg agcagcggaa gattgcgcac
     2281 atctcccacg atgatgaagc ccccctcgcg cacagacctg atcgcccctt gcagcacgcg
     2341 cacgaaatag tcaacgcccg gaaagtactg gacgacggaa ttgatgatta cgccgtcgaa
     2401 cgatttcggc tcgatgcctt caaagtcggt cgcctctctt tgcgcgagtt ggacgtgcgc
     2461 cgcgccgccg tccaacaact tcagatgttg gcgcacataa tccagagccg ctggtgaaaa
     2521 gtcccgtccc cagtacttag tgcaatgcgg ggcaaggcga aacagcagca gacccgtgcc
     2581 gcagcctatc tcaagaatgc tctcaggctt gcgtgcgagg actctctcca ctgtcgcgtc
     2641 gagccattcg cgcatctccg cttcgggaat ggcctctccg gtgtaactgc tcttccaccc
     2701 gctgatgttg aatgtcggct catcgctgat ggaagtctca ttagccactt cgtcattgtg
     2761 aacgtcctgc cattgcgtga ctcgctgagt tgccagcgcg acctttttgt cagcctctac
     2821 gtattgcagg ttctgcacta cataagccgc aagattctgg tggccgggaa tgtcttcacg
     2881 caccatgacg acggcctctt tcacggctgg at
//
LOCUS       DASWNS010000601         5438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_232212, whole genome shotgun sequence.
ACCESSION   DASWNS010000601 DASWNS010000000
VERSION     DASWNS010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232212"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..2758
                     /locus_tag="VGB76_20600"
     CDS             47..2758
                     /locus_tag="VGB76_20600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0101336.1"
                     /translation="MRKEEWREVEELLDAALELAPEERRKFLEEVGGRAPELRREIES
                     LLVCEEGANNFLAAPALAFSADFFDAPGNDAHGADMRAGQTVGHYRIRREIGRGGMGA
                     VFLAERSDGEFEQQVALKIVRRSFADSELARRFRRERQILASLNHPNIARLLDGGMSA
                     DGEPFFAMEYVEGVRIDDYCDAHDLPNEARLSLFLGVCRGVAYAHQNLVVHRDIKPSN
                     ILVNAEGTPKLLDFGIAKLLDADQLDEHTRTDFRAFTPDYASPEQVSGGHVTTASDVY
                     SLGVLLREILGGAKITGRASSQKEFVPGVWRSVTPGQKTRATNLPTNRENGDGRARTT
                     RRRFVAAELENIIAMARREEPARRYASVAQLAEDVQRYLDGRPTRAQRDSFTYRAGKF
                     IRRNKLSVGAGLIVALSLLVGTAFALWQARVARGERDRAERRFADVRQLSNALLTEIA
                     PKIERLPGSTEARQAVLTQSLKYLDSLAYESADDLSLQSELASAYEKIGDLQGNPTNP
                     NLSALTDALASYEKANAMRRALLARAPRDFEQRRLLADNYRSLGDIRWQANESAESLK
                     NSEAALSLYTELLAGQRGSNLLRISLARTHLSIGKSHATNERYPEAISSFQKAVTLLA
                     EAERPSPEQKIEISMLLADAHKQIGNSLSWDGKQPEAEAEMAQAVSISESLIAANPHD
                     NRLRTSLHQTYMTMSSVYEESNDPLSNEYAFKALKVIEETVARDPADLRARHELAQTY
                     SLLGVTLENIGRNGESILYLEKAVRILQEIMRNETKNRRFKYDLATALTRLGDAVHKQ
                     RRFRDSLAALQNAAAVLTDLTNSDPTDNASLRNLANAHDSMAKSHEALAARATTGAEK
                     QSHHQQAKQNYTRALELLHRLEAKNALSTYDRKSLEEMQTILQKYDR"
     gene            complement(2793..4409)
                     /locus_tag="VGB76_20605"
     CDS             complement(2793..4409)
                     /locus_tag="VGB76_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101337.1"
                     /translation="MSDKPNEKSRKTLKWRRDLAYLMQQERELSGLKTREEYPKHGDY
                     QKQVETQLKPPDLSYLLRLMMWLNYKQKPLPSRRRLRKLLILWCLMRVESIIENEERF
                     HKEVVTDYEIIPPKPEGTDKKANEEYKKEIDRIFQQKWKKSALEVQEYFEEMLYPVYQ
                     ELEGQRERIQVTPDRLPTLESFPEAFEPLVVVVGGYGGLVCREPEHISELFRQSQSFV
                     NLHYLLGLNLSPDTEIVSDRLLLELNNDRERATELLGNKHVLVIGSPLVNAFSRHLIL
                     KKKLIFNFVLRKETYRLGQDLYRDMLEGDADHNLLGEGFYDSMIKTKLLETPPALSMF
                     YRLMNKPPEEVDLRSPDYNGVEMAHRRQIEDMVFRIRELVGDSEATNEKVTNMFRPKE
                     MFSPLDRTLHDADGNSNQEFAVISLAENYWANLLRKKEEPPKFASIVVAGVNELSTTV
                     AVRALSKKSDLRERPLGGLLDIRKPPAPGLKRITGAGDKWLTLPYEAQDVLRKVDLAL
                     ESFERRPSAFNLFDDQRELGLYREIVKRHV"
     gene            4572..5087
                     /locus_tag="VGB76_20610"
     CDS             4572..5087
                     /locus_tag="VGB76_20610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY0101338.1"
                     /translation="MANETSPLVKLLSTIGFFTGLTIGWNSTQNGWAALVCGVIIAGL
                     GAFAGKVVHKLLVIAVSLLLAFGSAYMRHAVFKGLYKNGQQYSTSSPRTTYEKPDATP
                     TRARVIAANANLRSKPGDGTGSGNRLVGTVALGQRLDVLAPKYVGKGWYHVRHLELGE
                     GWVHGNDIEFE"
BASE COUNT         1145 a         1705 c         1518 g         1070 t
ORIGIN      
        1 attgcctgtc agttttccgt cccgaatccc gcgtgtacag gtagcgatgc gaaaagagga
       61 atggcgagaa gttgaagaac tgctggacgc cgcgttggaa cttgcgcccg aagagcggcg
      121 caagtttctc gaagaggtcg gggggcgcgc gccggagttg cggcgcgaaa tcgaatcgct
      181 gctcgtctgc gaagaggggg cgaacaattt cctcgccgct cccgcgctcg ccttctcggc
      241 agactttttt gacgctcccg gaaacgacgc gcacggcgcg gacatgcgtg cggggcagac
      301 cgtcggccac tatcgcatca gacgcgagat cgggcgcggc gggatgggcg cggtctttct
      361 cgccgaacgc tccgatggag agtttgagca acaggtcgcg ctcaagatcg tgcggcgcag
      421 tttcgccgat tcggaactcg cgcgccgctt ccgccgcgag cggcagatcc tcgcctcgct
      481 caaccacccg aacatcgcac gccttctgga cggcggcatg agcgcggacg gcgagccgtt
      541 cttcgccatg gagtacgtcg agggcgtgcg catcgacgac tactgcgatg cgcacgatct
      601 cccgaacgaa gcgcgtctga gcctcttcct cggcgtctgc cggggcgtcg cctacgcaca
      661 ccagaacctc gtcgttcacc gcgacatcaa gccgtcgaac atcctcgtca acgccgaagg
      721 cactccgaag ttgctggact tcggcatcgc caaactgctc gacgccgatc aactcgacga
      781 acacacccgg acagactttc gcgccttcac gcccgactat gcttcgcccg aacaggtgag
      841 cggcgggcac gtcacgaccg cctcggacgt gtacagcctc ggcgtgctgc tgcgagagat
      901 cctcggcggc gcaaaaatca cggggcgcgc ttcgtcgcag aaggaattcg tgcccggcgt
      961 ctggcgttcc gtgacgccgg gacagaagac gcgcgcgacg aatctgccga cgaatcgaga
     1021 gaacggagac gggcgggcga gaactacgcg caggcgtttc gtcgccgccg aactggaaaa
     1081 catcatcgcg atggcgcgac gcgaagaacc cgcaagacgt tacgcttccg tcgcgcaact
     1141 cgcagaggac gtgcagaggt atctggacgg gcgacccacc cgcgcgcaaa gagattcttt
     1201 cacctatcgc gcggggaagt ttatccggcg caacaaactc tcggtcggcg cggggctgat
     1261 cgtcgcgctc tcgctcctcg tcggcacagc cttcgcgctg tggcaggcac gcgtcgcgcg
     1321 cggcgagcgc gaccgcgccg agcgtcgttt cgcagatgtg cgccaactct cgaacgcgct
     1381 cctcacagag atcgcaccca agatcgaacg ccttcccggt tcgaccgaag cgcggcaggc
     1441 cgtccttacg caatcgctca aatacctcga cagcttagcg tacgaatccg ccgacgatct
     1501 ctcgctgcaa agcgaactcg cgtcggccta cgagaagatc ggcgacttgc agggcaaccc
     1561 caccaacccg aacctctccg cgctaaccga cgcgctcgcc agctacgaga aggccaacgc
     1621 gatgcgccgc gccctgctcg cacgcgcccc ccgggacttc gagcagcgtc gtctgctcgc
     1681 cgacaactat cgctcgctcg gcgatattcg ctggcaggca aacgagtctg ccgagtcgct
     1741 taaaaactcg gaagcggctt tgagtctgta tacagagttg ctcgcggggc aacgcggctc
     1801 aaacctgttg cgcatctctt tggcgcgcac ccacctcagc atcgggaagt ctcatgcgac
     1861 taacgagcga tacccggaag cgatctcttc ttttcagaag gccgtcaccc tgctggcaga
     1921 agcagagcgg ccatcgccgg aacagaagat agagatttcg atgttgctcg cggacgccca
     1981 caagcagatt ggcaactcgc tttcatggga cgggaaacag ccggaggccg aagccgagat
     2041 ggcgcaggcc gtgtccatct ccgaatcgct catcgctgcc aacccgcacg acaacaggct
     2101 gcgcacctcg ctccaccaga cttacatgac gatgagcagc gtgtacgaag agagcaacga
     2161 tccgctctct aatgagtacg cctttaaagc cctgaaagtc atcgaggaga ctgtcgcacg
     2221 cgaccccgcc gacctgcgcg ccagacacga actcgcccaa acctattccc tgctcggcgt
     2281 gacgctcgaa aacatcgggc gcaacggcga atcaattctc tatctcgaaa aagccgtccg
     2341 catcttgcag gagatcatgc ggaacgaaac gaagaaccgc cgcttcaaat acgatctcgc
     2401 caccgccctc acgcgcctcg gcgacgccgt gcacaaacag agacgctttc gcgattcgct
     2461 cgcagccctg caaaacgccg ccgccgtgct caccgattta accaactccg accctaccga
     2521 caacgcctcc ctacgcaatc tcgccaacgc acacgactcc atggcgaaat cccacgaagc
     2581 cctcgccgcc cgcgcaacga cgggcgcaga aaaacagtcg caccatcaac aggcaaaaca
     2641 aaactacacg cgcgccctcg aacttctcca ccgcctcgaa gccaaaaacg ccctctccac
     2701 ctacgaccgc aaatccctcg aagagatgca aaccatcctc caaaaatacg accggtaaag
     2761 ctcagcacgc actctttgtc gtcgcctccc ggctagacat gccgcttcac tatttccctg
     2821 tacagtccca actcgcgttg atcatcgaac aggttgaacg cacttgggcg tcgctcgaag
     2881 gactctaagg cgaggtcaac ctttctcaat acgtcctgag cctcataagg cagggtgagc
     2941 cacttatcgc ccgcccctgt gatgcgcttg aggccgggcg caggcggttt cctgatgtcg
     3001 agcagaccgc cgagcggtcg ctccctgaga tcgcttttct tgctcaacgc tctcacggcc
     3061 acggtggtag acaattcatt caccccggcg acgactatcg aagcaaactt cggaggctcc
     3121 tcttttttac gcagcaggtt agcccagtag ttctcggcca agctgatgac cgcgaactct
     3181 tggttgctat tgccgtcggc atcatgcagc gtgcggtcta agggggagaa catctcttta
     3241 ggccgaaaca tgttggtcac tttctcgttc gtggcctcgg agtcgccgac cagttccctg
     3301 atcctaaaga ccatatcttc gatttgccgc ctgtgcgcca tctcgactcc gttatagtcg
     3361 gggctgcgga ggtcaacctc ctcgggcggt ttgttcatca gccggtagaa catgctgagc
     3421 gcggggggcg tctctaacag cttggtcttt atcatgctgt cataaaagcc ctctcccaga
     3481 aggttgtgat cagcatctcc ctccaacatg tccctgtaca aatcctgccc cagccggtaa
     3541 gtctctttgc gtaaaacgaa attgaagatc agcttctttt taaggatcag gtggcgcgag
     3601 aaggcgttca ccagaggact gccgatgact aaaacgtgct tgttacccag cagttcggta
     3661 gcccgctccc tgtcgttgtt cagttcgaga aggaggcggt cggatacgat ctccgtgtcc
     3721 gggctgaggt tcaagcccag caggtaatga aggttgacga agctctgcga ctgccggaac
     3781 agctcggaga tatgttcagg ctcccggcac actaaccctc cgtacccgcc cacgacgacc
     3841 accagcggct cgaaggcttc gggaaacgat tcgagcgtcg gcaggcggtc gggggtgact
     3901 tgaatgcgct cgcgctgccc ttcaagttcc tggtataccg gatagagcat ctcctcaaaa
     3961 tattcctgca cttccagcgc agactttttc cacttctgct ggaagatgcg gtcgatctct
     4021 ttcttgtatt cttcgtttgc ttttttatct gtgccctcag gtttaggagg gatgatctcg
     4081 taatcggtga ccacttcctt atgaaagcgt tcctcatttt cgatgatgga ttcgacccgc
     4141 atcaagcacc agaggatgag cagcttgcgc agacgcctcc gagacggcag gggtttctgc
     4201 ttgtagttga gccacatcat gagccggagc aggtaggata ggtcaggggg tttcagttgc
     4261 gtctcaacct gcttttgata gtctccgtgc ttcgggtact cctcccgcgt cttaagtccg
     4321 ctcagttctc gctcctgctg cattaagtag gctaaatctc gccgccattt gagcgttttt
     4381 cgggattttt cattcggttt atcggacata agggaatcct cccggcagat atcgccgccg
     4441 ttcgcgatgc tccgagcagc ggcggctgat cattttatgc cccgagcact ccacggctcc
     4501 gagctttgtg ggacattacg ggtcatcggg cagaacccgc tcgatgatta aagttaagcg
     4561 aggagtgaac tatggcgaat gaaacttccc ctttggttaa gctgctctca accatcggct
     4621 tctttacggg actgacaatc ggctggaaca gtactcagaa cgggtgggcg gcactcgtct
     4681 gtggcgtcat cattgccgga ctgggagcct ttgccggtaa ggtcgtgcac aaactgctgg
     4741 tcatcgccgt ctcgttactg ctggcgttcg gaagtgcgta tatgaggcac gcagtcttta
     4801 agggactcta taagaacggt cagcaatata gcacaagtag tccgcgcaca acttacgaaa
     4861 aaccggacgc gacgcccacg cgtgcgcgcg tcatcgcggc gaacgcgaac ctgcgttcaa
     4921 agccgggcga cggcacaggg tcgggcaaca ggctcgtcgg gacggtggcg ctcgggcaga
     4981 ggctcgacgt gctcgctcca aagtacgtcg gaaagggctg gtatcacgtc cgccaccttg
     5041 aactaggcga aggatgggtg cacggaaacg acatcgaatt cgagtgactc gtcaagtcgg
     5101 aggcgactac tgctcccgct gcgacttgtc cggcaggccg gtgcgcgggg cagtgctttc
     5161 gccgcatgtc gatagtgcga gctttgcctt gccccgaacg tgtcccgtta acctcacttc
     5221 caagcgccca ccggtcgagc atcctacccc tgagcgcgtc ccaaacgtca atgctgaatt
     5281 ccctgacgcc gcagcgcagg gaacggcgat tcaaaacaag gagtaagaga tgaataacaa
     5341 cagagtaatc atggttggtt taaccagcgt tgccttcgtt tcccttctcg tcgtgtttac
     5401 ggcagcttac gtgagcaagg ccaaccagct ggcaatta
//
LOCUS       DASWNS010000602        15001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_515796, whole genome shotgun sequence.
ACCESSION   DASWNS010000602 DASWNS010000000
VERSION     DASWNS010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15001
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515796"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VGB76_20615"
     CDS             complement(<1..1264)
                     /locus_tag="VGB76_20615"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0101339.1"
                     /translation="MPVIFAAGTIPPFLTEVVALLVAGAVIAYVCYRIGLVPIVGFLL
                     AGVVIGPNALGLVRDRELVDAAAEVGVILLLFTIGIEFSLQKLARIKRLIFGGGGLQV
                     GLATLSTMGVLALFGVDWRAGLFTGFLVALSSTAIVLKLLGDRGETGERRGQVGLGLL
                     IFQDLAIIVMVLLVPVLSGSGGSTLGIVWALAKAGAIIAAVLIFARRLMPRMLEMVAR
                     TCSPELFLLTVIAICFGTAYLTSLAGVSLSLGAFLAGLLVSESRFSEHALSEIMPLQI
                     LFSATFFVSVGMLLDLSFLAQNLPLVLVAVAIVLVVKVATTGVSVLALGYRLPVAAAS
                     GLMLAQVGEFSFVLERAGREVGLSPAGLAGAGSQTFIASTVVLMVLTPLLMQVGSRLS
                     RRLETKQAVREASQIAAQPLPEHLPHLED"
     gene            complement(1375..3216)
                     /locus_tag="VGB76_20620"
     CDS             complement(1375..3216)
                     /locus_tag="VGB76_20620"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY0101340.1"
                     /translation="MSETNTLNKDYSFTRDREDARVVEEYLNPLPRSIVRPNEYELLD
                     GEWRFCLDLEDRGLSESWYLGHNYTGNAIWPGSIEAHLAQAKGELADTPAWQDTVVAW
                     YEREFSIPEDWCTESGCLVQVTFGACGYETRVWLNGHPLRTIEGEDVHLGEYTSFSYE
                     LPPEHLQPVNRLTVRISDSYDAEIPRGKQQSTVYKQGGIWYQTYTGAVRSVWLEPVGR
                     NRLRSRLNVISSFRDNFVSFDLTTRIHDPGRYSLRLVVSVRGSEQPVAIQEVQLPLET
                     GEKRQRVTVALPDALRWSPAMPNLYHLVAQLSGQAGTVSQIEAHFGLRTIEARGGSVY
                     LNNESLYLDGILYQPGTATFEEMRRHMLAMKQLGCNLVRVHIAGIDPRIYDLADEIGV
                     LLWVEVPSPHRSSQQSRENHWAEMMRMLVHIGSHPSVVIWSLYNEDWGAEDIATNVET
                     RLYIAHAYDYLRQNHPQLLVVDNDGWQHVSMEGRLKSDLLTAHLYTPDLNFWRELLER
                     LARGETEGVAVKPLIIGDPYFYIGQVPLVVSEWGGFGFVEYGGPDDQEDKASRIRAFK
                     RELRRHAIAGDVYTQATSIEEEDNGLIDPQTGALLVPAGLLNSDAPA"
     gene            complement(3470..4771)
                     /locus_tag="VGB76_20625"
     CDS             complement(3470..4771)
                     /locus_tag="VGB76_20625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101341.1"
                     /translation="MVLKLLTSPVFRGVVGVAGAALFLAAGWCALLTGASRMFSDYGV
                     RTGNLVAAETAVRMTPGDPQAHVARADLLADAAGAGEASLRREAVRSYSDSASLRPLD
                     YALWMSLGQSLEEAGQAEDALRAFAEATRLAPFYARPRWQLGNFLLRQGRVDEAFDHL
                     RRAAHADHARFPNLIDLAWRFYDGEVARVEAALRPETNAERLKLARFYVRQGEAGEAI
                     RLYRQLGGAVAQDDQKAMLADLFAAREFVEAHKIWSLSQPQEAGAEQAAPGRIYDGGF
                     EREIRLNEPGFGWQIVTANDSNVSVALDGGEMQEGKRSLRVDFNGASAPSAPVLSQLV
                     LVEAGRAYQLSFAARTKDLLTGGLPVVTVLDVSAPDQLVLAQSKNLPQGSEGWQTFSV
                     EFKTRQSTRAVRLSLQRQNCASAPCPAFGHLWLDAFTMTKL"
     gene            complement(4901..5641)
                     /locus_tag="VGB76_20630"
     CDS             complement(4901..5641)
                     /locus_tag="VGB76_20630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101342.1"
                     /translation="MLTKNQKITRLLSILLVFCITPLYVNAELLSVKANAELASAQTD
                     SGGRLRTTGNRQIDVNGNPAPSGTTILSGASLSTSEGTGATVDLPGLGRVNMSPNSRL
                     SINYTASSVDVTVTSGCATLDLTNANANGSITAPRGEATRTSANQRHLDVCYTDGAAA
                     PIVNQGAAAAAGAGAAGGAVAGAATATGAAVSGGLFGLGVPATVALGVASAAFAAGGV
                     YAVTRTPCRRGANLSPGVPRGRNDECRE"
     gene            complement(5810..7282)
                     /locus_tag="VGB76_20635"
     CDS             complement(5810..7282)
                     /locus_tag="VGB76_20635"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0101343.1"
                     /translation="MSGHKGSASSSSPLSGTAKLSRRLGAAIFYAALSLILLTAIPYG
                     TVETWSIALFEIIVFTLGALWMAEGALGGGFWLRREHASLLAPLVLVSLYAYLQSVVP
                     VGVTPLNLREVISADPYETRLFALQFFALTTFAALLLRYTTNRRRLLALVLTVIAVGI
                     TCALFGIIRQTAQRAGEQGFVLPSLTGGVGYAQFVGRNSFAFLMEMTLGLGLGLLAGV
                     ARQRIMLVVASLLPLWTALVLCNSRGGILSMLAQVIFAPLLYLIARPSTRRRKEGRDA
                     PPGSEWEVETSGWQRLSGSVLFKGALVFTLLVVTLTGTIWMGGAPLADRLETVSGELN
                     PEAADPTGASRRDIWRSTLNLIKARPLAGSGFGAYWIAISPHHEGSGALSPQQAHNDY
                     LELAAGGGLFGALLFLCFIYLFVKRARLQLRSPERFRRAVCFGALVGLFGVSVHSLVD
                     FGLHVTVNALVCLALVAIATANVEAAGDNPGAANLRRPHG"
     gene            7571..7762
                     /locus_tag="VGB76_20640"
     CDS             7571..7762
                     /locus_tag="VGB76_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101344.1"
                     /translation="MRRGYTEAPLWLRIFLIHDLLCDNCNLLYRGFATSGALRRAKRR
                     RRRTGGSNSIDSSGNSSER"
     gene            complement(8056..8640)
                     /locus_tag="VGB76_20645"
     CDS             complement(8056..8640)
                     /locus_tag="VGB76_20645"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEY0101345.1"
                     /translation="MTARHHLEPQTGVGFLLARGQLLKIIDPQGEQVSDLTAFAQEDK
                     SEWLSSGRTIDYANTIYLTTGHLLYSNRSRPMFTILEDTVGRHDFLLTPCSPDTFKIL
                     YDLHDYHPSCFENLTKNLAAFGITPDMIPTTFNIFMNVIVLPSGELRIDPPPSRAGDH
                     ILLRAEMDLIVGVTACSAEKSNNYSFKPIDVEVN"
     gene            complement(8749..9558)
                     /gene="gntA"
                     /locus_tag="VGB76_20650"
     CDS             complement(8749..9558)
                     /gene="gntA"
                     /locus_tag="VGB76_20650"
                     /inference="COORDINATES: protein motif:HMM:NF041366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis heme-dependent
                     pre-guanitoxin N-hydroxylase GntA"
                     /protein_id="HEY0101346.1"
                     /translation="MSVGTASIVKTNPFGTDELARAHSMYTAWRDGQLIHPLEPERRP
                     TPLSESVHQSFRAHVLDQEFSCIGGKSAIINNTYRFGLYPEMNTRGATAGLAYDLWEY
                     ERERPTLATDYATFIASFAAPVARDERDWERLLWAQLQSLHELDRAYHAWDATVSSDP
                     EDADFSFSFAGTAFFVIGLHPQSSRYARRFAWPTLVFNAHLQFERLREQGLFDRVRET
                     VRARELKLQGSLNPNLSNYGERSEARQYSGRAVEEEWRCPFRARLNDANDT"
     gene            9846..10925
                     /locus_tag="VGB76_20655"
     CDS             9846..10925
                     /locus_tag="VGB76_20655"
                     /inference="COORDINATES: protein motif:HMM:NF024896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0101347.1"
                     /translation="MVTNVADREDLERADGDRATRGTYLLTIRRVRFDRREAESFTDY
                     FRSIAAVCDVLVVDGSPPEVFAAHREAWSGVCRHEAVDPQYKYLNGKVNGIHTGVAIS
                     AHERIILADDDIRYTPGDLRRMLALLADYEMVRPQNYLSPLPFRARTEAARMLINRAW
                     IAEGDYPGTLGVRRSAMLRVGHYDGDVLFDNEEIVRHFRAQSAHVAYARDFFILKRPP
                     GWRKWLEQRPRQAYEDFVMRAKTAFFTLLPLTLALVWFVSGWRWALGFASAVAAGAVL
                     VAASGLSDGATRFFPARLCLYAPLWIAERVVSTYWAFYWRAAYGGYPFGDKLVAKGTG
                     RAWRVGGNDGGGRSGRAASNTPSEL"
     gene            10930..12444
                     /locus_tag="VGB76_20660"
     CDS             10930..12444
                     /locus_tag="VGB76_20660"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY0101348.1"
                     /translation="MEPQPPIPPLGDLPEADFRRLLHETADWIADYRAGIETRRVAPD
                     AVPNEIINSLPAVPPDAAAPLEEILRDFERVIMPGIVHWGHPSFLGYFATTTNAPGII
                     GEMLAAALTVSAMTWQTSPAATELEFVVLDWIRQMLSLPADFQGVVYDTASVAVLHAL
                     AAAREDSGFDIRRKGLAGRADIPQFTLYASDQAHSSLEKAAITLGLGEANVRRIPSDA
                     EYKMDVDALRRAVAADLHAGRHPLAVIATVGTTSATSVDPVDQIADLCRDRRIWLHVD
                     AAYGGALALLEEGRHVMCGVERADSVVFNPHKWLFVPLDFSALYVRRPETLRRVFSLV
                     PEYLRGDAERAREEVPNYMDYGIQLGRRFRALKAWFVISAFGREGLAARIREHCRLAA
                     LFASWVEADARFETLAPVLMGVVCFRARVPGQNACVEGGGVETPNASSDELNERLLAR
                     INATGAAYMTHTRLHDTLALRIAVGNVLTTERHLAGVWKLICNQLSEMSEEAAG"
     gene            12624..13685
                     /locus_tag="VGB76_20665"
     CDS             12624..13685
                     /locus_tag="VGB76_20665"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0101349.1"
                     /translation="MSINEEKLNQLLGKFVADFGAAFHAGLVVIGESLGLYKALAQAE
                     QPLTSAELAERTGTTERYVREWLCSQAAGGYVEYDAQTGRYFLTAEQAFALADEQSPA
                     YLPGAFLLAVSALKAVPKITEAFRTGEGFGWHQHDHGLFRGTEMFFRPGYAANLVNSW
                     IPAFDGLEAKLKAGAKVADVGCGLGASTILMAQAFPNSTFIGFDYHDKSIEMAQQKAA
                     EAGVADRVEFAVAKAKDYPRENYDFVTFFDCLHDMGDPVGAAAHVRESLAPDGTWMIV
                     EPFAEDRLEDNLNPVGRIYYSASTLLCTPASLSQEVGLALGAQAGEARLREVVSAGGF
                     KHFRRATETPFNLIFEAKP"
     gene            complement(13787..>15001)
                     /locus_tag="VGB76_20670"
     CDS             complement(13787..>15001)
                     /locus_tag="VGB76_20670"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY0101350.1"
                     /translation="FGLRRITESTITLFLILSPCLLVFYGQAIWQAMKYENSKPLASP
                     ARGAQKNRIVWVIFDELDQHLAFVNRPPSLHLPEFDRLRGEALVATNAVAPADETLLS
                     LPSLTIGRLVDKAEPISEDELAIRFHKSGETAHWSTQPTIFSQAREKGQNTAIIGWYL
                     PYCRLFNRVTTKCYGDSARNMVDSRDERIIYNMLQLSASSLEAVPLVKRLSGMRLQSI
                     PRGDAETVEGLILSYVGLLKESLSAVADPNLGLIFVHLSVPHPPTFFDREAGVFSSRK
                     ANSYLDGLALADRTLGDIRHAMEGAGVWDDTVVLISSDHPWRRNMWYGKKYYFNEEDE
                     SLIVSENQRRVPFILKLAGQSKAKVYESSFNTVLSKDLLLAIMQGEVANSESVVEWLE
                     TSKTRFAFDMAK"
BASE COUNT         2985 a         4572 c         4586 g         2858 t
ORIGIN      
        1 ggtcttccag gtgcggcagg tgctccggca atggttgtgc ggcgatttgc gacgcttcgc
       61 gcacggcctg ctttgtctcc aaacggcgcg agagccttga gcctacctgc atcaggagtg
      121 gcgtcagaac catcaggacg acggtggagg cgatgaaagt ctgcgagcct gccccggcga
      181 gtcctgcggg tgagaggcca acttctcttc cggctctttc gagcacaaac gagaactcgc
      241 ccacctgcgc cagcatcagc cccgaagccg cagcgaccgg cagcctgtaa cctaaggcca
      301 gcacgctcac gcccgtcgtc gccaccttca cgacgaggac gatggcgacg gcgacgagca
      361 cgagcggtaa gttctgcgcc agaaaactca agtcgagcag catccccacc gagacgaaga
      421 acgtcgcgct gaagagaatc tgcaagggca tgatctcgct cagggcatgt tcgctgaaac
      481 ggctttcgct caccagcagc ccggcgagaa atgcgccgag cgacaggctc acgcccgcca
      541 gactcgtcag gtaagcggtg ccgaagcaga tggcgatcac ggtcaggaga aacagttccg
      601 gcgaacaggt gcgcgccacc atctccagca tcctcggcat taaacgtctg gcgaagatga
      661 gcaccgccgc gatgatcgcg cccgccttcg ccaacgccca cacgatgccg agcgttgagc
      721 cgccggaacc gctgagcacc ggcaccagca acaccatcac gatgatcgca aggtcttgga
      781 agatcagtaa tcctaaaccg acctgtccac gccgttcgcc agtctcgccc cggtcgccga
      841 gcagcttcaa cactatcgcc gtggaactga gcgcgacgag gaatcccgtg aacagtcccg
      901 cgcgccagtc aacaccgaac aaggccagca cgcccatcgt cgagagcgtc gccagcccaa
      961 cctgcaagcc gccgccgccg aagatgagcc gcttgatccg cgcgagcttc tgcaaactga
     1021 attcgatgcc gatggtgaag agcagcagga tgacgccgac ttcggctgcc gcgtccacga
     1081 gttcgcggtc gcgcaccagc cccagcgcgt taggtccgat gacgacgccc gccagcagaa
     1141 agccgacgat gggaaccagg ccgatgcgat agcagacgta agctatgact gcgcctgcga
     1201 cgagcagcgc gaccacttca gttaaaaatg gcggaatcgt tccggcagcg aaaatgactg
     1261 gcatggatga atgttaatgt ggaaatgtgc tacatgggaa ggccatagcg aacctgaact
     1321 caccagcggg agactgtctg ccggagagag atcgaagagg agttcgatct gaggtcatgc
     1381 cggggcgtcg gagttgagca gtccggcggg cacgagaagc gcgcctgttt gtggatcgat
     1441 caagccgtta tcttcttcct cgatgctcgt cgcctgcgtg taaacgtcgc ccgcgattgc
     1501 gtgccgccgc agttcacgct tgaaggctct gatgcgcgac gccttatctt cctgatcgtc
     1561 cggcccgccg tactcgacga aaccgaagcc gccccactcg ctcaccacca gcggcacctg
     1621 cccgatgtag aaatacgggt cgccgatgat caggggtttc accgccacgc cttccgtctc
     1681 gccccgcgcc agacgttcca gcagttcccg ccagaagttg aggtcgggag tgtagaggtg
     1741 cgccgtgagc aggtcggact tgagcctgcc ctccatagag acgtgctgcc agccgtcgtt
     1801 atccaccacc aacaactgcg ggtggttctg gcgcaggtag tcgtaggcat gggcgatgta
     1861 gaggcgggtt tcgacgttgg tggcgatgtc ctccgcgccc cagtcttcgt tgtagaggct
     1921 ccagatgacg accgaaggat gagagccgat gtggacgagc atgcgcatca tctccgccca
     1981 gtgattttcg cggctctgct ggcttgagcg atgcgggctt ggcacttcca cccacagcag
     2041 cacccctatc tcgtcggcca gatcgtagat gcgcgggtcg atcccggcga tatgcacccg
     2101 caccagattg cagccgagtt gcttcatcgc cagcatgtgg cggcgcatct cttcgaaggt
     2161 ggccgtgccg ggctggtaga ggatgccgtc cagatacaga gactcgttgt tgagatagac
     2221 cgagcctccg cgcgcctcta tcgtgcgcag tccgaaatgc gcttcgatct gcgaaaccgt
     2281 ccccgcctgc ccgctgagtt gcgcgactag gtggtacagg ttcggcatgg cgggcgacca
     2341 acgcaaagca tcaggtaggg cgacggtcac gcgctgtcgt ttctctcccg tctccagcgg
     2401 caactgcact tcctgtatgg cgaccggttg ctcgctcccg cgcacgctta ccaccagccg
     2461 cagagaatag cgaccgggat catggatgcg cgtcgtcaga tcaaaggaga cgaagttgtc
     2521 tctgaaactg ctgatcacat tcaggcgtga gcgcaggcgg ttacgcccga ccggctccag
     2581 ccagacgctc ctgaccgccc ccgtataggt ttgataccag atgccgccct gcttatagac
     2641 ggtcgattgc tgcttgccgc gcggaatctc cgcatcgtag gaatcgctga tgcgcaccgt
     2701 caggcggttg acgggctgga gatgttcggg cggcaactcg tagctgaaag aggtgtactc
     2761 gccgaggtgg acatcctcgc cctcgatggt tctgagcgga tgaccgttga gccacacgcg
     2821 cgtctcgtag ccgcacgcgc cgaacgtgac ctgcacgaga caaccggact ccgtacacca
     2881 atcttccggg atagagaact cgcgctcgta ccacgcgacg actgtatcct gccacgcggg
     2941 cgtgtcggcc aactcgccct tggcctgcgc gagatgtgct tctatcgaac caggccagat
     3001 ggcgttgcct gtgtagttat gaccgaggta ccaactctcg ctcagcccac ggtcttcgag
     3061 gtcgaggcag aaccgccatt ctccgtcgag cagttcatac tcattggggc ggactatgga
     3121 gcgcgggagc gggttgagat actcctcgac gacgcgtgcg tcttccctgt cgcgcgtaaa
     3181 gctataatct ttgttcagcg tatttgtttc tgacatggcg ctcaagatgg tagtgctgca
     3241 aaaaatttct gtctacacct gctattgatc tatctacacc aaagattgag ttggcattac
     3301 tatcaacact atcaacgccc ttgctaattt tgcggcgaca atattttttt gccacattac
     3361 cgcagcgtcc gggtcgcacg ctattgcgtc gagcgcggtt cgctccggtt tgattgtttc
     3421 gtctgtggca acgtgcgcgg agaaaaagcg gaggggagag cgacggctct caaagtttcg
     3481 tcatcgtaaa ggcgtcgagc cagaggtggc cgaaggctgg acagggcgcg ctcgcgcagt
     3541 tctgtcgctg gagagagagg cgcacggcac gcgtgctctg gcgggtcttg aattcgacgc
     3601 tgaaagtctg ccagccttca gacccttgcg gcagattttt cgactgcgcg agaacgagtt
     3661 ggtcgggcgc actcacgtcg aggacggtga cgacgggcag cccgcccgtc aggaggtctt
     3721 tcgtgcgcgc cgcgaaactc agttggtaag cgcgacccgc ttcgaccaga acgagttgcg
     3781 aaagcaccgg cgcggagggc gcggacgcgc cgttgaaatc gacgcgtagg ctcctcttcc
     3841 cttcttgcat ctccccgccg tcgagcgcga cggacacgtt ggaatcattc gccgtcacga
     3901 tctgccagcc gaagcccggc tcgttcaaac gtatctcgcg ctcaaaaccg ccgtcgtaaa
     3961 tccgaccggg cgcggcctgc tctgcgcccg cttcctgcgg ttgcgagagc gaccagattt
     4021 tgtgggcttc gacaaactct cgcgccgcga agaggtctgc gagcatcgcc ttctgatcat
     4081 cctgtgcgac cgcgccgccg agttgacggt agagccttat ggcttcgccc gcctcgccct
     4141 gtctgacgta gaaacgtgcg agtttcaaac gctccgcatt ggtttcaggc cggagggcgg
     4201 cttcgacgcg cgcgacttcg ccgtcgtaaa aacgccaggc gaggtctatc aggttcggga
     4261 agcgcgcgtg gtcggcgtgc gcggcgcgtc tgagatgatc gaaggcttca tcgactctcc
     4321 cctgccgcaa caggaagttt cccaactgcc agcgcggcct cgcatagaag ggggcgaggc
     4381 gcgtcgcttc ggcgaacgcg cgcaaggcgt cttccgcctg accggcttct tcgagcgact
     4441 gacccaggct catccagagc gcgtagtcca gcgggcggag gctggccgag tcgctgtatg
     4501 agcggacggc ttcccgccgc agggaggctt cgcccgctcc ggcagcatcg gcgagtaggt
     4561 cggctcgtgc gacgtgagcc tgcgggtcac ccggcgtcat gcggaccgcc gtttcggcgg
     4621 cgacgagatt tcccgtcctc acgccgtaat cggagaacat gcgtgaggct ccggtcagga
     4681 gcgcgcacca gcccgccgcg agaaatagag cggctccggc gacgccgacg acgccgcgaa
     4741 agacaggaga ggtgagaagt ttcagaacca tgcgtttcag ggagaagcgt cgagataggt
     4801 cagtagaaaa aagcgcgtgc cggacggaca caaaaaaagc atgccccgct cagacgggcg
     4861 gggcatgcag gataacaggt tcgtcgcgcg gcgagcagga ttactcccgg cattcatcgt
     4921 tgcggccacg aggcacgccc ggactcagat tcgcgccgcg acggcaaggg gtgcgggtga
     4981 cggcataaac tccccccgca gcgaaagccg cagacgcgac gcccagcgca acggtggccg
     5041 ggacgcccag accgaagagt cctccgctga ctgcggcacc ggtagccgtg gccgcaccag
     5101 cgaccgcgcc gcccgccgca ccggctccgg cggcggcagc cgcgccctga ttaacgatgg
     5161 gagctgccgc gccgtccgtg taacaaacgt caagatgacg ttgattcgcg gaggtgcgcg
     5221 tcgcctcgcc tctcggtgcg gtgatggagc cgttcgcgtt cgcgttcgtc aggtcgagcg
     5281 tggcgcaacc ggaagtcacc gtcacgtcaa cactgctggc cgtgtagttg atgctgagtc
     5341 tgctgttcgg cgacatgttc acccgaccca gaccgggtag atcgacggtc gcgccggttc
     5401 cttcggaagt cgagagcgat gcgccggaaa ggatggtcgt gccggacggc gcggggttgc
     5461 cgttcacgtc gatctgcctg ttgcccgtcg tgcggagtct cccgccggag tcggtctgcg
     5521 cggaagcgag ttcagcattc gctttcacgc taagaagttc cgcattaacg tacagaggcg
     5581 tgatgcagaa gaccaacagg atggagagga gcctcgtgat tttttggttc tttgtgagca
     5641 taacgaattc tccgaatctt ctgaggtcaa tcttgtcaac ggagggttaa ttgagatgtc
     5701 tccgaacgtt gacttgaggg gagcatctgc gagtgtggag tgtagagtat tgagtgcgac
     5761 aggtcaacct tttcccattc gttttcccgg tattttcgcg cgacaaatct cacccgtgag
     5821 ggcggcgaag gttggccgcg ccggggttgt cgcctgccgc ctcgacattc gccgtcgcga
     5881 ttgcgacgag cgccagacag acgagggcgt tcacggttac gtgcagcccg aaatcgacga
     5941 gactgtggac ggagacgccg aagagtccga cgagcgcgcc gaaacagacg gcacgacgga
     6001 agcgttcggg agagcgaagt tggagccgcg cgcgcttaac gaacaggtag atgaaacaga
     6061 ggaaaagaag cgcgccgaag agtccgcccc cggcggcgag ttcgaggtag tcgttgtgcg
     6121 cctgctgcgg cgagagcgcg ccggagcctt cgtgatgtgg gctgatggcg atccagtaag
     6181 cgccgaatcc ggagcctgcc agcgggcggg ctttgatcag attcaaagtc gaacgccaga
     6241 tgtccctgcg gctcgccccc gtagggtctg cggcttcggg attcaactcg cccgacaccg
     6301 tctcaagccg gtcggcgagc ggtgcgccgc ccatccagat cgtacccgtg agagtgacga
     6361 ctaagagtgt gaagacgagt gcgcccttga acagcactga accggagagc ctttgccaac
     6421 ctgaagtttc aacttcccac tcactaccgg gcggcgcgtc gcggccttcc ttccggcgtc
     6481 gggtcgaggg tctggcgatg aggtagagca gtggcgcgaa tattacctgc gcgagcatgc
     6541 tcaatatccc gccgcgagaa ttacacagca cgagcgccgt ccagagggga agcaacgatg
     6601 cgacgacgag catgatgcgc tggcgcgcca ccccggcgag tagtccgagt ccgagtccca
     6661 acgtcatctc catcaaaaac gcgaagctgt tgcgcccgac gaactgggca tagcccacgc
     6721 cgcccgtgag ggacggcagc acgaagcctt gctcccccgc ccgttgcgcc gtctgccgga
     6781 tgatgccgaa caacgcgcac gtgattccga cggcgatgac tgtgaggacg agcgcgagca
     6841 ggcgtctgcg gttcgttgtg tacctgagga ggagcgcggc gaacgtagtg agcgcgaaga
     6901 actgtagtgc gaagaggcgc gtctcgtaag ggtcggcgct tatcacctcg cgcaagttca
     6961 agggcgtgac gcccaccggc acgacgcttt gcaggtacgc atagagcgag acgagaacga
     7021 gcggggcaag gagagacgca tgctcgcgtc ggagccagaa gccgccgccg agcgcgccct
     7081 ccgccatcca gagcgcgccg agcgtaaaaa cgatgatctc gaagagcgcg attgaccacg
     7141 tctcgaccgt cccgtaagga atggccgtga gcaggatgag cgagagcgcg gcgtagaaga
     7201 tcgcggcacc gagtctgcgg gagagtttgg ctgtgccgga aagaggcgaa gaagaggacg
     7261 cgcttccttt gtgcccgctc accgcgccgc tttgttcagg ttcgagagag gccaaaatca
     7321 tcaccccacg gaaggagagc cttgagcatc aaacaattcg aaactcaact tgacaggtcg
     7381 ccgctgcgac tactgctgtt atcgtcaggc gcgcatttat cgtcgaagga gctatgatga
     7441 ttgcgtctgg caaccgtgtg cggatgggac tatactggca agcgtcttcc cccaagtata
     7501 aaagtctcag cacgagggga cgaacgctgt gatatttgag ctgcactgtc ccaaatgcgg
     7561 aagttcgcgc gtgcggcggg gctatacgga agcgcccctc tggctcagaa tatttctcat
     7621 ccacgacctg ctctgcgaca actgcaacct gctctatcgc ggcttcgcca cgtcgggagc
     7681 actgcgtagg gcgaaacgca ggaggaggcg gactggcggc agcaacagta ttgacagcag
     7741 cggcaacagt agcgagcgat aacggcatgg gtcgcattta cagaggcgag cggtgctcgt
     7801 ttaattcttc ccgtctcgta agttcagttg attggcgaga gtgtgtgcgc agaagcgcgc
     7861 ggctcgtgta gacgtaagga cggttctccg gtcgcgcggc agcatcggag acaggcgtct
     7921 tccaacctac tgtgcgtcgc gcgcatggcg cggcaaaagc ccctcctcgc tcatgcccct
     7981 ttcgctcgca gggatgttca ccgccagccg gttcgccgga atccccgccc tcttcgattc
     8041 gcaccggaca cggactcagt tcacctcaac gtcaattggc ttgaagctgt agttgttgga
     8101 cttctccgcc gagcatgccg tgacgccgac gatcaaatcc atctcggcgc gcagcagaat
     8161 gtgatcgcct gcgcgcgagg gcggcgggtc tatgcggagt tcgccggacg ggagcacgat
     8221 gacgttcatg aagatgttga aggtcgtcgg gatcatgtcg ggcgtgatgc cgaaggcggc
     8281 caggttcttc gtcaggttct cgaaacaact cgggtgatag tcgtgtagat cgtagaggat
     8341 tttgaaggtg tcggggctgc acggcgtgag caggaaatcg tgacgcccca ccgtgtcttc
     8401 gaggatggtg aacatcgggc gactgcggtt cgagtagagc aggtggccgg tcgtcaggta
     8461 gatcgtgttg gcgtaatcaa tcgtgcgccc ggaggagagc cactcggatt tatcctcttg
     8521 cgcaaaggcg gtcaggtcgg aaacctgctc gccctgcggg tcgatgattt tcagcagttg
     8581 cccgcgcgcc agcagaaagc cgacgccggt ttgcggttcg agatggtggc gggcggtcat
     8641 cgtttaagtt cactcctcgc gcattgattg ataacgtttg atggcgtttg acgagtcgaa
     8701 gccagcgaag cgggtggcag gcggtgagcc gtcgcaaggc tttccggcct atgtatcgtt
     8761 cgcgtcgttg agacgggcgc ggaacgggca ccgccactct tcttcgacgg cgcgccccga
     8821 atactgtcgt gcctcgctgc gctcgccgta gttgctgaga ttagggttga gcgaaccctg
     8881 caacttgagt tcgcgcgcgc ggactgtctc acgcacgcgg tcgaacaagc cctgctcgcg
     8941 cagccgctcg aattggaggt gcgcgttgaa gacgagcgta ggccacgcga aacggcgtgc
     9001 gtaacgcgaa ctctgcgggt gcaggccgat gacgaaaaag gccgtccccg cgaagctgaa
     9061 ggaaaagtct gcgtcctccg ggtcgctgct gacggtcgcg tcccaggcgt gatacgcgcg
     9121 gtcgagttcg tgcaagctct gtagttgcgc ccacagcagt ctctcccaat cccgctcgtc
     9181 gcgcgcgacg ggcgcggcga aactggcgat gaaggtggca tagtccgtcg ccaaagtcgg
     9241 acgctcccgt tcgtactccc acaggtcgta agccagaccg gcggtagccc cgcgcgtgtt
     9301 catctccggg tacaggccga agcgataagt gttgttgatg atggccgact tcccgccgat
     9361 gcaggagaac tcttgatcga gcacgtgcgc gcgaaaactt tggtggactg actcggagag
     9421 cggcgtcggg cgacgctcag gctcaagcgg gtgaatcagc tgtccgtccc gccacgcggt
     9481 atacatgcta tgtgcgcgcg cgagttcgtc cgtgccgaac ggatttgtct taactatcga
     9541 agctgtccct actgacataa ctgcttgctc gtaccctaaa cgctcatcgc tctcaaacag
     9601 gatgcaacgt gttgccgctg ctaagcgatc cgcttgcccg gcgcgagagg cggtaaatta
     9661 tctcaaaaca cggggagccg tttgaatatt aacgcaataa tcatgtcgaa cccgtgtcat
     9721 cacactatct ttcgttcata aagaccggga taaaatgagc cgcgtttggg tacgtaaacg
     9781 tacagtctaa agcgaccggg cgtgtcaaca ttcgtgactt cccaaattac ccagaggcaa
     9841 gccatttggt aacgaacgtc gcagacagag aagatcttga gcgggcggac ggcgaccgcg
     9901 cgacacgggg cacctacctg ctgactattc gccgcgtgcg ctttgaccgg cgcgaggcgg
     9961 agagtttcac agactacttc cgttccatcg ccgccgtttg cgacgtgctc gtcgttgacg
    10021 gttcgccgcc agaggttttc gccgcgcacc gtgaggcgtg gagcggcgtg tgccgccatg
    10081 aggctgtaga cccgcaatac aagtacctga acggcaaagt caacggcatc cacacgggcg
    10141 tcgccatctc ggcgcacgag cgcatcatcc tcgccgacga cgacattcgt tatacgcccg
    10201 gcgacctgcg acgcatgctc gcactgctcg cggattatga gatggtgcgc ccgcaaaact
    10261 acctcagtcc cctgcccttc cgggcgcgca cggaggcggc tcgcatgctc atcaaccgtg
    10321 cgtggatcgc cgaaggcgac taccccggca cgctcggcgt gcgccgcagc gcgatgctcc
    10381 gcgtcggcca ttacgacggc gacgtgctct tcgacaatga agagatcgtg cgccacttcc
    10441 gcgcgcaaag cgcgcacgtc gcttacgcgc gcgacttctt catcctcaaa cgcccaccag
    10501 gctggcggaa gtggctggag cagcgacccc gtcaggctta cgaagatttc gtgatgcgcg
    10561 cgaagaccgc ttttttcacc ctgctgccct taacgctcgc gcttgtctgg ttcgtctcgg
    10621 gttggaggtg ggcgctcggt ttcgcgtcgg cggtggcggc gggcgcggtg ctcgtcgcgg
    10681 cgagcggcct gagcgacggg gcaacgcgtt tcttccccgc acgcctctgc ctctacgcgc
    10741 ccttatggat cgcggagcgc gtcgtcagca cctactgggc gttctactgg cgcgccgcat
    10801 acggcggcta cccgttcggc gacaaactcg tcgccaaagg cacgggtcgg gcgtggcgcg
    10861 tcggcggcaa cgacggcggc ggcaggagtg gccgcgccgc gtcgaacacc ccttccgaac
    10921 tttaaccgag tggaacctca acctcccatt ccccctctcg gcgacctgcc cgaagccgac
    10981 ttccgccgcc tgctgcacga gacagccgac tggatcgcag actatcgcgc cggtatcgag
    11041 acgcgccgcg tcgcgccgga cgccgtgccg aacgagatca taaacagtct gcccgccgtc
    11101 ccgcccgacg cggcagcgcc gctcgaagag attctgcgcg acttcgaacg ggtcatcatg
    11161 cccggcatcg tccactgggg gcacccttcc ttcctcggct acttcgccac cacgacgaac
    11221 gcgcccggca tcatcggcga gatgctggcc gccgccctca ccgtctctgc aatgacgtgg
    11281 cagacttctc ccgccgccac cgaactggag ttcgtcgtcc tcgattggat acgacagatg
    11341 ctctctcttc ccgcagactt tcaaggcgtc gtctacgaca cggcctccgt cgcagtcctc
    11401 cacgcgctcg ccgccgcacg cgaagactca ggcttcgaca tccggcgtaa agggctggcc
    11461 gggcgcgcag acatcccgca gttcacactc tacgcctcgg accaggcgca cagttcgctg
    11521 gagaaagcgg ccatcaccct cggactcggc gaagcgaacg tgcggcgcat cccttcggac
    11581 gccgagtaca aaatggacgt ggacgccctc cgccgcgccg tcgcagcgga cttgcacgca
    11641 ggccgtcatc cgctcgccgt cattgccacc gtcgggacga cttcggcgac gagcgttgac
    11701 ccggttgacc aaattgcaga cctgtgccgc gaccgacgca tctggctgca cgtcgatgcc
    11761 gcttacggcg gcgcgttggc gctccttgaa gaaggccgcc acgtgatgtg cggcgtcgag
    11821 cgcgccgatt ccgtcgtctt caatccgcac aagtggctct ttgtgccgct cgacttcagc
    11881 gcgctctacg tgcgtcgccc cgaaacgctc cgccgagtct tctcacttgt ccccgaatac
    11941 ctgcgcggcg acgccgaacg cgcccgtgaa gaggtgccca actacatgga ctacggcatc
    12001 caactcggac gccgcttccg cgcactcaaa gcgtggtttg tcatcagcgc cttcgggcgc
    12061 gagggactcg ccgcacgcat ccgcgaacac tgccgcctcg cagcgctctt cgcctcgtgg
    12121 gtcgaggccg acgcgcgttt cgagacgctc gcgcccgtcc tgatgggcgt cgtctgcttc
    12181 cgcgcccgcg tgcccggcca gaatgcgtgc gttgaaggcg gcggtgttga gactcccaat
    12241 gcgtcatcgg acgagttgaa cgagcggctc ctcgcgcgca tcaacgcgac cggcgcggcc
    12301 tacatgacgc acacgcgcct gcacgacaca ctcgcactgc gcatcgccgt cggcaacgtg
    12361 ctcaccaccg agcggcacct cgcgggtgtc tggaagttaa tctgcaacca gttgtcagag
    12421 atgagcgaag aggcggcggg gtgaaaggtc ttgaaaggtt ttgcaaggtt tcggagtgtg
    12481 ccgcactttt gagcgcgaga gttgccccgc ctgctcgcgg cctcaacgaa tgcagatgtg
    12541 ctgaccaaaa ttgttgacgc tcggccaact ttgagataaa agcatcccgc ctcgattatc
    12601 tactcgcgac gggagagagt tgaatgtcta tcaatgaaga aaagttgaat caattattgg
    12661 gaaagtttgt cgcagacttc ggcgcggcat tccacgccgg tctggttgtg atcggcgaga
    12721 gcctcggtct ttacaaggcg ttggcgcagg cggagcaacc tcttacttcc gccgaattag
    12781 ccgagcgaac cggcacgacc gagcgttacg tgcgcgagtg gctctgctca caggcggcgg
    12841 gagggtacgt cgagtatgac gcgcagacgg ggcgttactt cctcacagcg gagcaagcgt
    12901 tcgctttggc cgacgaacag agtccggcat atttacccgg agcttttttg ctggccgtct
    12961 ccgcgctgaa agccgtgccg aagattacgg aagcatttcg cacgggcgag ggtttcggtt
    13021 ggcatcaaca cgatcacgga ttgtttcgcg gcaccgaaat gtttttccgc cccggttacg
    13081 cagccaacct ggtcaactca tggattcccg cttttgacgg gcttgaggcc aagctcaagg
    13141 cgggggcgaa agtagcagat gtcgggtgcg ggcttggcgc ttcgacaatc ctcatggcgc
    13201 aggcattccc caactctact ttcatcggtt tcgactatca cgataagtcc atcgaaatgg
    13261 cgcagcagaa ggcggctgag gcgggggtgg ctgaccgggt ggagttcgcc gtcgctaaag
    13321 ctaaagacta ccccagagaa aactacgact tcgtcacgtt cttcgattgc ctgcacgaca
    13381 tgggcgaccc cgtcggcgcg gcagcccacg tcagggagtc gctcgccccc gatggcacgt
    13441 ggatgatcgt cgagcctttt gccgaagacc gattggaaga taatctgaat ccggtcggac
    13501 gcatctatta ttccgcttcg acattgctct gcacccccgc ctctctgtcg caggaagtag
    13561 gacttgcgct cggagcgcaa gcgggcgagg cgaggctgcg cgaggttgtt tccgccggag
    13621 gattcaagca ctttcgccgc gccaccgaaa cgcccttcaa tctgatcttc gaggcgaagc
    13681 cctaacgaat ttaagagggg cggttcaaat caagctgccc ctcacaacgc atccaacccg
    13741 ccgtcgcgag taccgcgagt ccttcgccga aggcgcgggc gtaaatctac ttcgccatgt
    13801 caaatgcaaa tctggtttta gatgtctcca accactcaac aacgctttcc gaattagcaa
    13861 cttcgccctg cattattgcg agcaacaaat ctttactaag aacagtgtta aaggacgact
    13921 cgtaaacctt cgccttggat tgtcccgcga gcttaagtat aaacggcacc ctgcgttggt
    13981 tctcacttac aataagtgat tcgtcctcct cattaaaata gtacttcttg ccataccaca
    14041 tattccttct ccaagggtgg tcgctggaga tgaggacgac agtgtcgtcc cacactccgg
    14101 ctccttccat agcatgacgt atatctccca gagtacggtc tgccagtgcc agtccatcta
    14161 gataactgtt tgctttacgc gacgagaaca cgccagcctc tctatcgaaa aaggtgggcg
    14221 ggtgaggaac tgacaaatgg acaaatatca atcccaggtt agggtcagcc acagcactca
    14281 gcgactcctt caacaagccg acataggaca gaataagtcc ttccactgtt tcggcatcgc
    14341 ctctaggtat actttggagc cgcatacccg agagccgctt gaccagaggc accgcctcaa
    14401 ggctggaggc agataactga agcatattat agattattct ttcatcacgg ctgtctacca
    14461 tattcctggc cgaatccccg tagcacttag tcgtcacccg gttgaacaat ctgcaatagg
    14521 gaagatacca gccgataatg gccgtatttt gtcccttctc gcgggcttgt gaaaatatgg
    14581 tcggctgcgt gctccaatga gccgtctctc cacttttgtg aaagcggata gctaactcat
    14641 cctcgctgat cggttctgct ttatctacaa gcctaccgat tgttaaggac ggcagcgaga
    14701 gcagcgtctc atcggcagga gcgacagcat tggttgcgac cagcgcctca ccgcgcaatc
    14761 tatcaaactc cgggagatgc aacgagggag gccgattcac aaatgcaagg tgctggtcga
    14821 gttcgtcaaa aattacccac acgatgcgat ttttttgcgc ccctcttgca ggtgatgcta
    14881 aaggtttcga attttcatat ttcatcgcct gccagatggc ttgtccgtaa aagaccaata
    14941 ggcacgggga taaaattaag aacagcgtga tcgtgctctc tgtaatacgt cttagcccga
    15001 a
//
LOCUS       DASWNS010000603         6056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_308045, whole genome shotgun sequence.
ACCESSION   DASWNS010000603 DASWNS010000000
VERSION     DASWNS010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308045"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1526)
                     /locus_tag="VGB76_20675"
     CDS             complement(165..1526)
                     /locus_tag="VGB76_20675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0101351.1"
                     /translation="MAYEGQPTPQPTRRERLNHEQRILLTALLGGLPGTLISLILLWD
                     RPYTIKVQWTLTLLICGFWLGFAFSLRGRVVRHLQTLSNLLAALREGDYSIRARGAGR
                     EDALGDVMLEVNALGETLRAQRLGAMEATALLRTVMSEIDVAVFAFDEEERLRLVNRA
                     GERLFAQPSVRLLGRTAVELGLEEFLHFSRADDSQIIPKTFPGRAGRWGVGRSAFRER
                     GLPHQLLVLSDLSRELREEEREAWKRLVRVLGHELNNSLTPIKSIAGSLESLLVREPR
                     PLDWHEDMQRGLSIIGSRSESLSRFMQAYAHLARLPPPRFAPIDVGALVRRVAELETR
                     LPVKILPGAELVVQADGDQLEQLLINLQRNAVDAALQTGGGVSVSWTKNTNHFTLSIK
                     DEGPGLSNTTNLFVPFFTTKPGGSGIGLVLSRQIAEAHGGTLTLTNRRNGTGCEARLQ
                     LPL"
     gene            complement(1516..2889)
                     /locus_tag="VGB76_20680"
     CDS             complement(1516..2889)
                     /locus_tag="VGB76_20680"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0101352.1"
                     /translation="MKTNEPNIPRVLVADDQLDVLEALRLLLKGEGYQTEAVTSPSDV
                     LVAIKAKEFDLLLIDLNYTRDTTSGQEGLDLLSRVQMLDASLPVVVMTAWGSVEVAVE
                     AMRRGARDFIQKPWENARLLSVLRTQIELSRALRKGQRLEAENKLLRDGHTPQTMIAT
                     AASMRPVLQLIESVGASDANVLVTGENGTGKEVVAQTLHAISPRAVKAMVTVNVGALS
                     EGTFESELFGHVKGAFTDAKTDRVGRFELADGGTIFLDEIANVPLPQQAKLLRIVETG
                     EFERVGSSKTRRVDVRILSATNADLREEVAGGRFRQDLLFRLNTVEIRLPPLRERRED
                     IPLLAHHFLNIYARRYRKPLTTFSGEATRSLLNYEWPGNVRELDHAVERAVLMARGEQ
                     VEAADLNLQPGREAERGLEEMSLDEVECFLIRKTLARFDGNVGQAAEALGLSRSALYR
                     RLQRYGL"
     gene            complement(2996..5446)
                     /locus_tag="VGB76_20685"
     CDS             complement(2996..5446)
                     /locus_tag="VGB76_20685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0101353.1"
                     /translation="MGTLIQDLRYGARMLWKSPGFTGVAVVVLALGIGANTTIFSLIN
                     GLLLRPIVGVESPERLAAVYTSDYSSGLYGSSSYPDYLSFREGANAFEGLAAYEQAVA
                     SLTGNDEPQRLRGEAVTSNYFKVLGVGASAGRTLLPEDESAASGNPVIVISHNLWQRR
                     FNSDPNLVNSTIHLDGRAHTVVGVAAENFRGLHMGLPPEFWQPLIPRVNNTSARGSRG
                     LEIVGRLKEGATPEQANAELTAIFARLAQEYPETNRGTLERPNEPRPVTVVSESRFNP
                     QQRKTIAGVTGMLMLMVGLVLLIACANVANLLLARASARRREIAIRLALGSGRFRLVR
                     QLLTESMCLAMLGGFLGLLLTLWTSDILPKFFPAGEASGLDLGVDWRVLGFTLGLSLL
                     TGIVFGLVPALQASRPNLLSALKDDTSAPEVRGFGRFGLRNVLVVLQVALSLVLLICA
                     GLFMRSLRAASTADPGFNAENVLLTRMERRGAKMEQEEEQLFYRRVREQVGALPGVRS
                     VALAYIVPVSGGGMRTGVSVEGYQPQPKEDTELNTNIISPDYFKTLGIPLLQGRDFSA
                     EDRKGAPGVVIINEEFARRYFPGANPLGRRIRKGSDESYLEVVGVVKNARYRGLVEEP
                     LPFFYTPQAQKGMSSMTLFVRTEGDPLTVLPAVRAELKNLDKNLTLYQINTFSAHLAD
                     ALSNERMVAVLLSVFGVAALLLAAVGLYGVMSYVVVQRTHEIGVRMALGAQPGDILKM
                     VLRQGMTLLLAGGLIGLTAAFALTRLMSSLLYGISATDPLTFVGITSLLSFVTLLACY
                     IPARRATKVDPMIALRYE"
     gene            complement(5607..>6056)
                     /locus_tag="VGB76_20690"
     CDS             complement(5607..>6056)
                     /locus_tag="VGB76_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101354.1"
                     /translation="RRVYSSLLLEQGNLSGEQLAKLLTQNSRAISSDYEKATLLIQVL
                     KQSLSDREVRAAFFTMLDTLSSDYERGRVLAVLLQRDDVATETIILALKSAGRMSSDF
                     EKANVLIRASKRGAANESVRVAVIEAAHALGSDHERGRVLTAATKKQ"
BASE COUNT         1155 a         1889 c         1875 g         1137 t
ORIGIN      
        1 acgctcgatt attgaaaatt ttgattgagc aactatagcg cgtctgtgtg gcaaagcatt
       61 cacggggaaa cgatgaagga ggaacgatga attgaagaca agagcttttg ctttacttca
      121 tcgttcatcg ttcatcgttc atccttcctc gcttcctctg tgctttagag agggagttgg
      181 agtcgcgctt cgcagcctgt gccgttccta cggttggtga gggtcagcgt gccgccgtgc
      241 gcttcggcga tctggcggct gaggacgagg ccgatgccgg agcctcccgg cttcgtggtg
      301 aagaaaggga cgaagagatt cgtcgtgttg gaaagccccg gcccctcgtc tttgatggag
      361 agggtgaagt gattcgtgtt cttggtccaa ctcacggaga cgccgccgcc cgtttgcagc
      421 gcggcatcca cggcgttacg ttgcaagttg atcaggagtt gttcgagttg gtcgccgtcg
      481 gcctgcacca ccaactctgc gccggggaga attttgacgg gcagccgcgt ctcaagttcc
      541 gccacccgac gcaccagcgc gccgacatca ataggcgcga aacgcggcgg gggcaggcgc
      601 gcgagatgcg cgtaagcctg catgaagcgg ctcagggatt cggaacgcga gccgatgatg
      661 gagaggccgc gctgcatgtc ttcgtgccag tcgagcgggc ggggttcgcg cacgagcagg
      721 ctctccagac tccctgcgat ggatttgatc ggcgtcagag agttgttcaa ctcgtggccg
      781 agcacgcgca cgagtcgctt ccaagcctcg cgttcctctt cgcgcagttc gcggctgagg
      841 tcggagagga cgagcaattg atgcggcagg ccgcgctcgc gaaacgcgct gcgccccaca
      901 ccccaacgcc ccgcgcgacc gggaaatgtc ttcgggatga tctgcgaatc gtcggcgcgc
      961 gaaaaatgta gaaactcttc gaggccgagt tcgacggcgg tgcgaccgag gagacgcacg
     1021 gaaggctgcg cgaagagccg ctcgcccgca cgattgacga ggcgcaggcg ttcctcttcg
     1081 tcgaaggcga agacggcgac atcgatctcg ctcatcaccg tgcgcagcag cgcggtagcc
     1141 tccattgcgc cgagccgctg cgcgcgcaaa gtctcgccca gcgcgttgac ctccagcatc
     1201 acgtcgccga gggcgtcttc gcgtcccgcg ccgcgcgcgc gaatcgaata gtcgccttcg
     1261 cgcagggcgg cgaggaggtt cgagagcgtt tgcaggtggc gcaccacgcg cccgcgcagg
     1321 gagaaggcga agccgagcca gaagccgcag atgaggagcg tgagcgtcca ctgcaccttg
     1381 atcgtgtagg gacgatccca gagcaggatg agcgagatga gcgtgccggg caggccgccg
     1441 agcaaggccg tgaggagaat gcgctgctcg tggttcagtc tttcgcgccg ggtgggttgc
     1501 ggtgtcggtt ggccttcata agccataacg ttgaagtcgt ctgtagagcg cgctccggct
     1561 gagtccgagt gcttccgccg cctgcccgac gttgccgtcg aagcgcgcga gagttttgcg
     1621 aatcaggaaa cactctacct catcgaggct catctcttcg agtccgcgtt cggcctcgcg
     1681 tcccggctgc aaattgagat cggcggcttc aacctgttcg ccccgcgcca tcaacaccgc
     1741 gcgctccacc gcatggtcca actcgcgcac gttccccggc cactcgtaat tcagcagcga
     1801 ccgcgtggct tccccggaaa acgtcgtcag gggtttgcga tagcgacgcg cgtagatgtt
     1861 gagaaaatgg tgtgcgagca gaggaatgtc ttcgcgccgt tcgcgcagag gcggcaagcg
     1921 tatctcaacc gtgttcagac gaaaaaggag gtcttgacga aaccgcccgc ccgcgacctc
     1981 ttctcttagg tctgcgttgg tcgcggaaag gatccgcacg tcaacgcgcc gcgtcttcga
     2041 agaaccgacg cgttcaaact ctcccgtctc gacgatgcgc agaagttttg cctgttgcgg
     2101 cagcgggacg ttggcgatct cgtcgagaaa gatcgtcccg ccgtctgcca actcgaaccg
     2161 cccgacgcga tccgtcttgg catcggtaaa cgcgcctttg acatggccga acagttcgct
     2221 ctcgaaagtg ccttcggaga gcgcgccgac gttgaccgtg accatcgctt tcaccgcacg
     2281 cggcgagatg gcgtgtagcg tctgcgcgac aacctctttg cccgtgccgt tttcgcccgt
     2341 cacgagcacg ttggcgtcag atgcgccgac gctctcgatg agttggagca cggggcgcat
     2401 gctcgcggcg gtggcgatca tggtttgcgg cgtgtgcccg tcgcgcagca acttgttctc
     2461 ggcttcgagg cgttgacctt tgcgcagcgc gcggctcagt tcgatctgcg tgcgcagcac
     2521 ggagagcagg cgcgcgttct cccagggctt ctgtatgaaa tcgcgcgcgc cgcgccgcat
     2581 cgcttccacc gccacttcga cgctgcccca tgccgtcatc accaccacag gcaagctcgc
     2641 gtcgagcatc tggacgcgcg agagcagatc gagtccttcc tgcccggagg tcgtatcgcg
     2701 cgtgtagttg agatcgatca ggagcagatc gaactctttc gctttgatcg cgacgagcac
     2761 gtccgatggc gacgtgacgg cctccgtctg atacccttcg cctttcagca acaggcgcag
     2821 ggcttcgagc acgtcgagtt gatcatctgc gacgagcacg cgcggaatat tgggttcatt
     2881 cgttttcatt cgttgaagtg agccgcgtag ataaaagcgc gctcacggct tccatgttat
     2941 tcattaattt tagcgcggac gcaactcaaa agcccctccc cggcgcggga gatgcctact
     3001 catagcggag cgcgatcatc ggatcaactt tcgtcgcgcg tcgcgccggg atgtaacagg
     3061 ccagcagcgt gacgaaagac agcagcgaag taatccccac gaaagtcaga gggtcggtcg
     3121 cgctgatgcc gtagagcaag ctcgacatga gccgggtcag agcgaacgcg gcggtgagtc
     3181 cgatcaagcc gccggcgagc aggagcgtca tgccctggcg cagcaccatt ttcaggatgt
     3241 cgcccggttg cgcgccgagc gccatccgca cgccgatctc gtgcgtgcgc tgcaccacga
     3301 cgtaagacat cacgccgtag agtccgacgg cggccaacag caacgctgcc acgccgaaca
     3361 cgctgagtag aaccgcgacc atccgctcat tggataacgc gtcggcgagg tgcgcggaga
     3421 aagtgttgat ctggtagagc gtcaggttct tgtcgaggtt cttcaactcg gcgcgcactg
     3481 ccgggagcac cgtcaagggg tcgccctccg tgcgcacgaa gagcgtcatc gaggacatcc
     3541 ccttttgcgc ctgcggcgtg tagaagaacg gcagaggctc ctcgaccaag cccctgtacc
     3601 gtgcattctt caccacgccg accacctcta aataagattc atccgaaccc ttcctgatcc
     3661 gcctgcccag aggattcgcg ccggggaaat aacgcctcgc aaactcttcg ttgatgatga
     3721 cgactccggg cgcgcccttc ctgtcctccg cgctgaagtc gcggccttgt aggagcggaa
     3781 tgccaagcgt cttgaaatag tcggggctga tgatattcgt gttgagttcc gtgtcctcct
     3841 tcggctgcgg ttgatagcct tcgacggaga cacccgtgcg catcccgccg ccgctgacgg
     3901 gaacgatgta agccagcgcg accgagcgca cgccgggcag cgcgcccact tgctcacgca
     3961 ccctgcgata gaagagttgc tcctcttcct gctccatctt cgcgccgcgc ctctccatcc
     4021 gcgtgagcag cacattctcc gcgttgaaac ccggatcagc ggttgaagcg gcgcgcaggc
     4081 tgcgcatgaa cagtccggcg cagatgagca ggacgagcga cagcgcgacc tgtaagacga
     4141 cgagcacatt gcgcaacccg aaccgaccga acccgcgcac ctcaggcgca ctcgtgtcgt
     4201 ccttcaaagc cgacagcaga ttcgggcgcg aggcttggag cgcgggcaca aggccgaaca
     4261 cgatcccggt cagcaaggat aggccgagcg tgaagccgag cacgcgccag tcaacgccga
     4321 ggtcaagccc gctcgcttct ccggcgggaa agaatttcgg caggatgtcg gaagtccaga
     4381 gcgtcaacag gagtccgaga aaaccgccga gcatcgccag gcacatgctc tcggtcaaca
     4441 attgccgcac gagccggaag cgacccgaac cgagcgcgag ccggatggcg atctcgcgtc
     4501 gtcgcgcgga ggctcgcgcc agcaacaggt tcgccacgtt ggcgcaggcg atcaagagca
     4561 ccagcccgac catcaacatc agcatccccg tcaccccggc gatggttttg cgttgctgcg
     4621 ggttgaagcg cgattcagac acgacggtaa cggggcgcgg ctcgttcggt cgctcaagcg
     4681 tgccccggtt cgtttcggga tattcctgtg cgaggcgcgc gaagatggct gtgagttcgg
     4741 cgttcgcctg ttcgggcgtc gctccttcct tgagtcgccc gacgatttcg aggccgcgac
     4801 tgccgcgcgc actcgtgttg ttcacgcgcg gaatcaacgg ctgccagaac tccggcggca
     4861 agcccatgtg caagccgcga aaattctccg ccgccacgcc gaccaccgtg tgcgcgcgcc
     4921 cgtcgagatg gatcgtgctg ttgacgagat tgggatcgga gttgaaccgt ctttgccaga
     4981 ggttatggct gatgacgatg accggattac cgctcgccgc actctcatct tccggcaaga
     5041 gcgtgcgacc ggcggaagcc ccgacgccga gcactttgaa atagttgctc gtcactgcct
     5101 cgccacgcag acgttgcggc tcatcgtttc cggtcaggct cgccacggcc tgttcgtaag
     5161 ccgcgagtcc ttcgaaggcg ttcgcccctt cacgaaaaga gaggtagtcg ggataagagg
     5221 aactgccgta aaggccgctg ctgtaatcgc tcgtataaac ggcggcgagt cgctccggcg
     5281 attcgacgcc gacgattggc cggagcaaca gcccgttgat caggctgaag atcgtcgtgt
     5341 tcgccccgat gccgagcgcc agcaccacga ccgccacccc tgtaaagccc ggactcttcc
     5401 acagcatccg cgcgccataa cgcaagtctt ggatcagtgt tcccatctgc aaacctctat
     5461 ctttaagtga cgagtgacaa gtgacgagtt aagacaggtt ctttctaagt gaagagcgac
     5521 gaacaggaac taatgtgtga cgggcaggaa caaagctcct gttttaacct gtcacctgtc
     5581 actcgtcact cgtcacttgt cactcgtcac tgcttttttg tcgcggcggt gagcacacga
     5641 ccgcgttcgt gatccgagcc gagcgcgtgc gcggcttcga tgacggcgac gcgcacggac
     5701 tcattcgccg cgccccgctt cgacgcgcga atcaacacgt tcgccttctc gaagtccgaa
     5761 ctcatcctgc ccgcagattt gagcgcgaga atgatcgtct cggtcgccac gtcgtcgcgt
     5821 tggagcagga cggccagcac gcgcccgcgc tcgtaatcgg aagagagcgt gtcgagcatg
     5881 gtgaagaagg ccgcgcgcac ctctctgtcc gagaggctct gcttcaaaac ttgtatgagc
     5941 agcgtggcct tctcgtagtc cgaactgatc gcgcgcgaat tctgcgtgag caacttcgcc
     6001 aactgctcgc cgctcaggtt tccctgttcg agcagcaggc tcgaatagac gcgccg
//
LOCUS       DASWNS010000604         9244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_100611, whole genome shotgun sequence.
ACCESSION   DASWNS010000604 DASWNS010000000
VERSION     DASWNS010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100611"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VGB76_20695"
     CDS             complement(<1..582)
                     /locus_tag="VGB76_20695"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEY0101355.1"
                     /translation="MNEHYAGLDLPAYRGAAANMSFRFAKAGLATRTMNDSWYNAGLG
                     SAAEAQMKNALRQGSADDLNFYTTGGGGYLGWATFPNEYATRPKMDGVICYWATLPGS
                     NYAPYNEGDTGTHEAGHWLGLYHTFQGGCQGAGDGVADTPAERTNVFGCPTRALDTCK
                     SNAGLDPYENFMDYTDDPCMYKFSAGQVDRADSM"
     gene            571..900
                     /locus_tag="VGB76_20700"
     CDS             571..900
                     /locus_tag="VGB76_20700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101356.1"
                     /translation="MLVHRNDLSVEIRFGNVANPIRRINDVEIDRDDDGTRRVDAGAI
                     GGGAATAALTAAVGRAVFLNVLVDLLNFDVVGVARLALSAGVEEVFMGFKNGAVGGDD
                     SAVTSDS"
     gene            complement(1258..2871)
                     /locus_tag="VGB76_20705"
     CDS             complement(1258..2871)
                     /locus_tag="VGB76_20705"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0101357.1"
                     /translation="MATQFQEVSGTRQRHEQQPAEIISYDPATGEEVGRAHVFSTAEV
                     AQAVERARTAQKVWGALGYAERGRIMLRARALVLEEMDAIAHLISRESGKPATEALMM
                     ELVPTLDLIQFFARRTGRLLKDERIGLGQYNLMGRSSRIVYKPLGVVGIISPWNFPWA
                     IPLGEVVMSLMAGNAVVLKPSELTPLVGQKIGEVFKRAGLPDDLLSVVTGAGATGAAL
                     VEAGVDKIMFTGSVATGRRVAEAAARKLIPCVLELGGKDPMLVVEDANVEAAAQAAVW
                     GAFANAGQACASVERCYVHEEIAERFTARVVELTRALKQGEGAREGTDIGAMSSVRQR
                     EIVEEQLRAALAQGARILAGGGRGENPAGTFHAPTVVDRVDHTMELMREETFGPVLPL
                     MTFKTEEEAIARANDSPYGLTASVWTRDIRRGERVAERLEAGTVMVNEVLYTHGIAQT
                     PWGGVKLSGIGRTHGRAGLLELVVAQHIHVNRLARLHDLWWFSYTPRAAELFRGFARR
                     FTTGSVIQTSLLLPQMLRRFSERRGKKKH"
     gene            3064..4368
                     /gene="pepP"
                     /locus_tag="VGB76_20710"
     CDS             3064..4368
                     /gene="pepP"
                     /locus_tag="VGB76_20710"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF008131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEY0101358.1"
                     /translation="MPRPQLAEFMRRMEPDSIAIIPSAREANRSNDTDYRYRQDSDFF
                     YLTGFNEPEAIAVVAPSHPEHKFMLFVRPRDPEREIWDGRRAGVEGARREYGADAAHT
                     FQEFKDKLPELLNGPANLYYKLGANSTLDEMIVRELRNLRANPRTAKHAPDHIIEPGT
                     LLHEMRLIKSEEEIALMQRSADIASEAHREAMRAARPGMKEYEIEALIEHIFRRHGAT
                     APAYTSIVGAGANATILHYINNDGTLEDGDLLLIDAGAEYQGYASDITRTFPINGRYT
                     QAQREVYELVLKAQVSCIERARPGVTMDELKKHSIEILTEGMVRLGLLQGEPEKLIEE
                     EKYKQFYMHGLGHYLGLDVHDVGPYYARGTKQGRPLEAGTVITIEPGIYIAPDTQNIP
                     EQYRGIGIRIEDDVLITPEGNRVLTTAAPKQIEEIEALMRKQ"
     gene            complement(4481..5371)
                     /locus_tag="VGB76_20715"
     CDS             complement(4481..5371)
                     /locus_tag="VGB76_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101359.1"
                     /translation="MRRLTEIVLSLALFAALAGCHAQDGSLARTGGAAQSQTGTAPKP
                     VSERQREAAARNPVPVIHVFVALCDNVNQGIVPVSARLGNGEDPANNLYWGAAFGVKT
                     FFSRSKDWKLLTSISNPQPAILERLVFKHAREQLFLVADAYRGSEIKQATIDFLEAAS
                     GSAGDALGVNVPASAAPVNFHIAGSARLVAYVGHDGLMDFRLPHHPQKKDDEPRDAII
                     LACASKNYFAPALKETGATPLLWTTNLMAPEAYILERAIDGWIKKESGAQIRKRAAEV
                     YHKYQHCGLKGAMNLFATGY"
     gene            complement(5503..6474)
                     /locus_tag="VGB76_20720"
     CDS             complement(5503..6474)
                     /locus_tag="VGB76_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101360.1"
                     /translation="MKLRLLYHGHCFDGVASAAVFTRFYRTRMKSSEGVDVQYTPLLH
                     RPGSLFDEEMFDGDENAIVDFKYSSSDRLTWWFDHHQSAFLTPEDEAHFHADRSGRKF
                     WDADSKSCTEFIARIAHEQFGYSDAGLAPLIEWAHIIDGAFYESPAQVVELRAPALQL
                     MQVIENVPDDWFLEKLIRELAERSLDEVATSAEVQERLHPILLRHRETLEVVRRKIAY
                     ARGVVTFDLVDEGHEAFNKFIPYYLHPETTYSVAVTRTSKRTKISVGSNPWSPRPRTH
                     NIAKICERYGGGGHAVVGAVSYGTDELERARATAREIIDELSSDFRE"
     gene            complement(6658..>9244)
                     /locus_tag="VGB76_20725"
     CDS             complement(6658..>9244)
                     /locus_tag="VGB76_20725"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0101361.1"
                     /translation="SRIPVNGFIILPFSGTYSILATSFAPEARGAYTLTLTAGANCSL
                     TPIAFGETRQGTLSAGDCTNAIDLDGTLVDLYTFNGTAGQQVSITMTAPAGGGNVDPY
                     LYLLLPNGDLIAEDDNSGGGTAARIPPIGEFGRLPVTGTYTIVANTVATNQSGNYNLT
                     LTQGATCASTAIALGGTANGTLANGDCRLLEDGSFLDAYTFNGTAGQQVVISLTSNTN
                     SLVPVIFLLAPNGDTLAIERSPTGSDTARVPVGSGSFALPANGTYTILVNSFAAGQTG
                     NYTLRLINPAGCTSTLAETARAVSGSGGQFTVGVTSQPGCTLTALSNSNFITINSSNI
                     DANGTGTVTYTVAANTTDAPRTGTLTINNQTFTVTQDAACVYVVSPSVRPFRNDGGNG
                     RFTVVTTNGCAWTATSNAPWITITSGFTSGTGTGRVRYTVAANTTSATRVGTITVNGV
                     AHTVTQTSAGTTPMIQFSSSTYTVNENDASRAATITVTRTGDLNGAATVEYATVDNPE
                     AVPCDPTVKRPDGTLYPQGTAYARCDYATTVDTLTFGIGETAKTFTIPLINDVHLEGN
                     EIFRISLSNAQGATLGGQATADVTIQFDDTAPPTGNPINQTPFFVRMQYLDFLSREPE
                     PSEPWSAVLNNCPNRFNAAGNFNDPSAGCDRIITSQSFFGSPEFRFKGLPIFLYYKAS
                     FGAANNPNYYPEYDQFAPDLRRVSGATAAEVFAKRLDFAEDWVARTAFVNAFAGTTNE
                     AFVDRLLANLNITLTAADPATGETRNSLVNSLNAGTKTRADVLRIIVESTEAANAQFN
                     QAFVAMQYFGYLRRTPDASGYQAWLNVLNNPAIDPRARPRLMIDGFMNSTEYRFRFGP
                     NTTQP"
BASE COUNT         1745 a         2785 c         2907 g         1807 t
ORIGIN      
        1 catcgagtcg gcgcggtcta cctgacctgc gctgaacttg tacatgcagg ggtcgtccgt
       61 gtagtccatg aagttttcgt aaggatcgag tcctgcgttc gacttgcagg tgtccagcgc
      121 cctcgtggga cagccgaaga cgttcgtgcg ctcggcgggg gtgtccgcga cgccgtcgcc
      181 cgcgccctgg cagccgcctt ggaaagtgtg gtagaggccg agccagtgac cggcttcgtg
      241 cgtgcccgtg tcgccttcgt tgtagggagc gtagttggag ccggggaggg tcgcccagta
      301 gcagatcacg ccgtccatct tgggtctggt cgcgtattca ttcgggaagg tcgcccagcc
      361 gaggtaaccg ccgccgccgg tggtgtaaaa gttcaggtcg tcggcactgc cctgacgcag
      421 cgcattcttc atctgcgctt cggcagccga gccgagtccc gcgttatacc acgaatcgtt
      481 catcgtgcgc gtggcaagtc cggccttggc gaaacggaac gacatgttgg cggcagcgcc
      541 tctataagcg gggaggtcta aaccggcgta atgctcgttc atcgcaacga tctgagcgtc
      601 gagattcgct tcgggaatgt tgccaacccc attcgacgca taaacgacgt ggaaatagac
      661 cgggatgatg acggaacccg ccgggtagat gcgggcgcca tcggtggagg tgccgccacc
      721 gccgccctga ccgccgccgt tggccgcgcg gtttttctca atgtgcttgt tgacctgctc
      781 aatttcgatg tcgtcggcgt ggcgcgtttg gcactttctg ccggcgtcga ggaagtcttt
      841 atgggattca aaaacggtgc cgtcgggggc gacgattctg ccgtcacttc tgatagctga
      901 ttgagaaccg ggatcgtccg gacgaccttg cgcgccagca tttttgaggg gggcgctgga
      961 gagcaggaat gcgaagctga ttgcaaacag aactaaggcg agcgtgaact ttttcattct
     1021 ttcctccgaa gctaagttaa tattgtgagt gggaagggtg ggacggtccc aaccaagaga
     1081 gcgggcacta tactacttat gcgagttaat taacagcttt tttttaagac agccggagca
     1141 ataaccagcc aaaaacagcg tgcctctctc cgtaacagaa aaagaacaaa tctcgcggca
     1201 ctgagatgct ttaaaggcga accgctcgac tccgctcgtg aaccgtctcc cggctcttca
     1261 atgcttcttc ttccctctgc gttcggaaaa acggcgtagc atctggggca gcaagagcga
     1321 cgtttggatg accgagccgg tcgtgaagcg gcgcgcgaag ccgcgaaaca gttcggcggc
     1381 gcgcggcgtg taactaaacc accagagatc atgcaggcgc gccagccggt tgacgtgaat
     1441 gtgctgcgcc acgacgagtt ccagtaatcc tgcgcgcccg tgcgtgcgcc cgatgccgga
     1501 gagcttcacg ccgccccagg gcgtctgcgc gatgccgtgc gtgtagagca cttcgttgac
     1561 catcaccgta cctgcttcca gacgttccgc gacgcgctcg ccccgcctga tgtccctggt
     1621 ccacacgctc gcggtcaaac cgtaggggct gtcgttggcg cgtgcgatgg cctcttcctc
     1681 cgtcttgaaa gtcatcaacg gcagcaccgg cccgaaagtc tcctcgcgca tcaactccat
     1741 cgtgtgatcg actctgtcca ccacggtcgg cgcatgaaac gttcccgccg ggttttcacc
     1801 gcgaccgccg cccgcgagga ttcgcgcgcc ctgcgccaaa gcggcgcgca actgttcttc
     1861 gacgatctcg cgttgccgca cgctcgacat cgcgccgatg tccgtgccct cccgcgcgcc
     1921 ctcgccctgc ttgagcgcac gcgtcaactc gacgacgcgc gccgtaaacc tctccgcgat
     1981 ctcttcgtgc acgtagcaac gctcgaccga cgcgcatgcc tgccccgcgt tcgcaaacgc
     2041 gccccacacc gccgcctgcg ccgccgcttc gacgttcgca tcttcaacca cgagcatcgg
     2101 gtctttgccg ccgagttcga gcacgcacgg gatcagtttt cgcgccgccg cttcggccac
     2161 gcgccgcccc gtcgccacgc tgcccgtgaa catgatctta tccacgcccg cttcgacgag
     2221 cgccgcgccc gtcgctcccg cgcccgtcac gaccgacagt agatcgtccg gcagtcccgc
     2281 gcgcttgaag acctcgccga tcttctgccc gacaagcggc gtcaactcgc tcggcttcaa
     2341 gaccaccgcg tttcccgcca tcagcgacat cacgacttca ccgagcggga tcgcccaggg
     2401 gaagttccac ggcgaaataa ttcccaccac gccgagcggt ttatagacga tgcgtgacga
     2461 gcgacccatc aagttgtact gaccaagacc gatcctctcg tctttcaaca ggcgacctgt
     2521 gcgccgcgcg aagaactgta tgaggtcgag cgtgggcacg agttccatca tcaacgcttc
     2581 cgtcgcgggc ttcccggact cgcgcgagat cagatgcgcg atagcatcca tctcttcaag
     2641 cacgagcgca cgcgcgcgga gcatgatgcg cccgcgctcc gcgtaaccga gcgcgcccca
     2701 taccttctgc gccgtacgcg cacgctccac cgcctgcgcc acctccgctg tggagaagac
     2761 gtgcgcgcgt cccacctctt cgcccgtcgc cggatcgtac gagatgatct ctgcgggttg
     2821 ctgctcgtgc ctctggcgcg tcccgctcac ttcctgaaat tgagttgcca taagatttgt
     2881 gtgtcgtcgt cttgctgttc cggcgaggta cgacaaatta tcatccgaaa acatctctgt
     2941 gggaagcgtc ggcaagacgc ccgcgtgcgt ggctcttcat gcgcccgcgc ttcttcggtt
     3001 acaatgctgg taataatgct cgcactacct taacacgctc gattgtttcc gaaggagaga
     3061 cttatgccac gaccacaact cgcggagttt atgcggcgca tggaaccgga ttccatcgcc
     3121 atcattccaa gcgcgcgcga agccaatcgc tccaacgaca cggactatcg ctatcgtcag
     3181 gattccgact tcttctacct gacgggcttc aacgaaccgg aagccatcgc cgtcgtcgcg
     3241 ccttcacatc ccgaacacaa gttcatgctc ttcgtgcgcc cgcgcgaccc ggaaagagag
     3301 atttgggacg gcaggcgcgc cggagttgaa ggcgcgcgca gagagtacgg agcggatgcc
     3361 gcgcacacct ttcaggagtt caaagacaaa ctgcccgaac tcttgaacgg ccccgcgaat
     3421 ctctattaca aactcggcgc aaacagcacg ctcgacgaaa tgatcgtgcg agaactgcga
     3481 aatttgcgcg ccaacccgcg cacggccaaa cacgcccccg accacatcat cgaacccggc
     3541 acgctcctgc acgagatgcg cctcatcaag tcggaagaag agatcgcgct tatgcaacgc
     3601 tccgccgaca tcgcatcgga ggcgcaccgc gaggcgatgc gtgctgcgcg accggggatg
     3661 aaggaatacg agatcgaagc tctcatcgag cacatcttcc gccgccatgg cgcaactgcc
     3721 cccgcctaca cttccatcgt cggcgcgggc gcaaacgcca ccatcctcca ctacatcaac
     3781 aacgacggca cgctcgaaga cggcgacctg ctgctcatag atgcgggcgc ggaatatcaa
     3841 ggctacgctt ccgacatcac gcgcaccttc cccatcaacg gtcgctacac gcaagcgcaa
     3901 cgcgaagtct acgaactcgt gctcaaggcg caggtgtcct gcatcgagag ggcgcgtccc
     3961 ggcgtgacga tggacgaact caaaaagcac tcgattgaaa ttctgacgga aggcatggtg
     4021 cgactcggac tgttgcaggg cgagccggag aagttgatcg aagaagagaa gtataagcag
     4081 ttctacatgc acggcctcgg ccactatctc ggcctcgacg tgcacgacgt agggccatac
     4141 tacgcgcgcg gcacgaaaca gggacgaccg ctcgaagccg gaaccgtgat caccatcgaa
     4201 cccggcatct acatcgcccc cgatacgcag aacatccccg aacaatatcg cggcatcggc
     4261 atccgcatcg aagacgacgt actcatcaca cccgaaggca accgcgtcct caccaccgcc
     4321 gcccccaaac agatcgaaga gattgaagcg ttgatgagaa agcagtgacg agaaagacaa
     4381 gtgatgagta atgagtgatg agtgatgagt gagaaccgaa gacttgtctt tcactcatca
     4441 cccatcactc atcacttctt tctcgtcacc cgtcactgtt ttagtatcct gtggcgaaga
     4501 ggttcattgc gcctttgagg ccgcagtgct ggtatttgtg atagacctct gcggcgcgct
     4561 tgcgaatctg cgcgccgctc tcctttttaa tccaaccgtc aatcgcccgc tctaaaatgt
     4621 aggcttcggg agccatgaga ttggtcgtcc agaggagcgg cgtcgcgccg gtttctttga
     4681 gcgcgggggc aaagtagttt ttgctggcgc aggcgaggat gatggcgtcg cgcggttcgt
     4741 cgtccttttt ctgcgggtgg tgcgggagac gaaaatccat cagcccgtcg tggccgacgt
     4801 aggcgacgag acgcgcgctc ccggcgatgt ggaaattgac gggcgcagca gacgcaggga
     4861 cgttgacgcc gagcgcgtcg cccgcgctgc cggaagcggc ttcgagaaaa tctatcgtcg
     4921 cctgtttgat ctcgcttccg cgataagcgt ctgcgacgag gaagagttgt tcgcgtgcgt
     4981 gcttgaagac gaggcgttcg agaatggcgg gctgcgggtt ggaaatgctt gtgaggagct
     5041 tccagtcttt gcttcggctg aagaaggtct tgacgccgaa ggctgcgccc cagtagagat
     5101 tgttggccgg gtcttcgccg ttgccgagcc gcgcggagac gggcacgatg ccttgattga
     5161 cgttgtcgca gagcgcgacg aagacgtgta tcacaggcac cgggttgcgc gccgcggcct
     5221 cgcgctgcct ctcgctcaca ggcttgggcg cagtgcccgt ctgactctgc gccgcgccgc
     5281 ccgtgcgcgc caaactcccg tcttgagcgt ggcagcccgc gagcgcggcg aacagggcga
     5341 gggataacac aatctccgtc aagcgtctca tggctgtttc gctcctcacc ctagatatag
     5401 acaccacgag ccggaagttg ttccgtaaac tctacactca ctcacacaac tcactcacga
     5461 aagtcactcg cgcaagccat acacgaaggt cattcacgaa ggtcattcac gaaagtcact
     5521 cgataactcg tcgatgatct cgcgggcggt ggcgcgtgcg cgttcgagtt cgtctgtgcc
     5581 gtaggagact gcgccgacga cggcgtggcc gccgccgccg tagcgttcgc aaattttggc
     5641 gatgttgtgt gtgcgcgggc gcggactcca cgggttcgag ccgacggaga ttttcgtgcg
     5701 cttcgaggtg cgcgtgacgg ccacggagta ggtcgtctct ggatgaagat aatagggaat
     5761 gaacttgttg aaggcttcgt gcccttcgtc tacgagatcg aaggtgacga cgccgcgtgc
     5821 gtaagcgatc ttgcggcgga cgacttcgag cgtctcccgg tgtcgcaaga ggatcgggtg
     5881 gagacgttcc tgcacttccg cgctcgtcgc cacttcgtcg agcgagcgtt cggccaattc
     5941 gcggataagc ttttcgagaa accaatcgtc gggcacgttc tcgatcacct gcatcaattg
     6001 cagcgcgggc gcgcgaagtt caaccacctg cgcgggcgat tcataaaacg cgccgtcgat
     6061 gatgtgcgcc cattcgatga ggggcgcaag tccggcgtcg ctgtagccga actgttcatg
     6121 cgcgatgcgt gcgatgaact cggtgcagga tttgctgtcg gcgtcccaga atttccgacc
     6181 cgaacggtcg gcgtgaaaat gggcttcatc ttcgggcgtg aggaaggccg actggtgatg
     6241 gtcgaaccac caggtgagtc tgtccgaaga cgaatatttg aaatcgacga tggcgttctc
     6301 gtcgccgtcg aacatctctt cgtcgaagag cgagccgggg cggtgcagga gcggcgtgta
     6361 ctgcacgtcc acgccctcac tgctcttcat gcgtgtgcga tagaagcgcg tgaagaccgc
     6421 cgccgacgct accccgtcga agcagtggcc gtgatagagc aaacgtagtt tcatctgtgc
     6481 ccgtactgaa aagagatttg acgacaagac agcacgcgat tttagcagca ctcgcgacgc
     6541 gcgcgaagga cgcaagcccg gaggagccgt gcggcttgcc ggaaaagcga aatgcggaga
     6601 ggaaaacttt cgctctcccc tccgcacttc gttttttccg atccctgcct gagttgctta
     6661 cggctgggtc gtgttaggac caaagcggaa gcgatactcg gtcgagttca tgaagccgtc
     6721 gatcatcagg cgtgggcgcg cacgcgggtc gatggccggg ttattgagca cgttgagcca
     6781 cgcctgatag ccggacgcgt ccggcgtgcg gcgtagatag ccgaagtatt gcatggcgac
     6841 gaacgcctga ttgaactgcg cgttcgcggc ctcggtgctc tcgacgatga tgcgcaagac
     6901 atctgcgcgc gtcttggtcc cggcgttcag cgagttgacg agcgagttgc gcgtttcgcc
     6961 ggtagcaggg tcagccgccg tcagagtgat gttgaggttt gcgagcaagc ggtcgacaaa
     7021 ggcttcgttg gtcgttccgg cgaaggcgtt gacgaaggcc gtgcgcgcca cccaatcttc
     7081 ggcgaagtcg agccgcttgg cgaacacttc cgccgccgtc gcgcctgaga cgcgccggag
     7141 gtctggggcg aactggtcgt attccgggta gtagttcggg ttgttcgctg cgccgaagga
     7201 ggctttgtaa tagaggaaga tgggcaagcc cttgaagcgg aactccggcg agccgaagaa
     7261 cgactgcgag gtgatgatgc ggtcgcaccc ggcggacggg tcgttgaaat tccccgccgc
     7321 attgaagcgg ttcgggcaat tgttcaagac agccgaccac ggctcgctcg gttcgggttc
     7381 gcgcgagagg aagtcgagat actgcatgcg cacgaagaag ggcgtctggt tgatcggatt
     7441 gcccgtcggc ggcgccgtat cgtcaaactg gatcgtcacg tcggcggtgg cctgcccgcc
     7501 gagcgtcgcg ccctgcgcgt tgctgagcga gatccggaat atctcgttgc cttcgaggtg
     7561 cacgtcgttg atgagcggga tggtgaaggt cttcgcggtt tcgccgatgc cgaaggtgag
     7621 cgtatcgacg gtcgtggcgt agtcgcagcg cgcgtaggcc gtgccttgcg ggtagagcgt
     7681 gccgtccggc cttttaacgg tcgggtcgca gggcacagct tccggattgt caaccgtggc
     7741 gtattcaacc gtggccgcgc cgttcaggtc gcccgtgcgc gtgacagtga tggtggccgc
     7801 cctcgacgcg tcgttttcgt tgaccgtgta ggtcgacgaa ctgaactgaa tcatgggcgt
     7861 cgtaccggcg gaagtttgcg tgacggtgtg cgcgacgccg ttcacagtga tcgtgccgac
     7921 gcgcgttgcg ctggtcgtgt tggccgccac cgtgtagcgc acgcggcctg tgcccgtgcc
     7981 gctcgtgaag ccggaggtga tcgtgatcca cggcgcgttc gacgtggccg tccaggcgca
     8041 cccgttcgtc gtcacgaccg tgaagcgacc gttgccgccg tcgtttctga acgggcgtac
     8101 gctcggagag acgacgtaga cgcaggccgc gtcctgcgtg acggtgaagg tctggttatt
     8161 gatcgtgagc gttcccgtgc ggggcgcgtc cgtcgtgttc gcggcgacgg tgtaggtcac
     8221 cgtgccggtg ccgttggcgt caatgttcga agagttgatg gtgatgaagt tcgagttgct
     8281 gagcgcggtc agagtgcagc ccggctgcga cgtgacaccg acggtgaact ggccgccgct
     8341 gccgctgaca gcgcgcgccg tctcggcgag cgtggaggtg cagcccgccg gattgatgag
     8401 gcggagcgtg taattgccgg tctgcccggc ggcgaaggag ttgacgagaa tcgtgtaagt
     8461 gccgtttgcg ggcagagcga atgagcctga cccgacgggc acgcgcgccg tgtcgctgcc
     8521 ggtgggcgat ctttcaatcg ccagcgtatc gccgttgggc gcgagcagga atatgacggg
     8581 aaccagcgag ttcgtgttgg aagtcaacga gatgacgacc tgctgccccg ccgtgccgtt
     8641 gaacgtgtaa gcgtcgagga acgaaccgtc ttcgagcagg cggcaatcgc cgttggcgag
     8701 cgtgccgttg gccgtgccgc cgagtgcgat ggccgtggag gcgcaggtcg cgccctgcgt
     8761 cagggtcaga ttgtaattgc cgctctggtt agtggcgacc gtgttggcga cgatggtgta
     8821 ggttcccgtc acgggcagcc tgccaaactc gccgatgggc ggaatacgcg ccgccgtgcc
     8881 gccgccgctg ttgtcgtctt cggcgatcag gtcgccgttc ggcagaagta gatagaggta
     8941 aggatcgacg ttgccgccgc ctgccggggc ggtcatcgtg atggagactt gctgccccgc
     9001 cgtgccattg aaggtgtaga gatcaaccag cgtgccgtcg agatcaatgg cgtttgtgca
     9061 gtcgcccgcg ctgagcgtgc cctgtctggt ttcgccaaac gcgatgggcg tcaaactaca
     9121 gtttgcgcca gccgtcagcg tgagggtata agccccgcgc gcttccggtg cgaatgaagt
     9181 ggcgagaatc gaataggtgc cgctgaaggg caggatgatg aaaccgttaa cggggatgcg
     9241 cgaa
//
LOCUS       DASWNS010000605         8370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_308110, whole genome shotgun sequence.
ACCESSION   DASWNS010000605 DASWNS010000000
VERSION     DASWNS010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308110"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VGB76_20730"
     CDS             <1..1345
                     /locus_tag="VGB76_20730"
                     /inference="COORDINATES: protein motif:HMM:NF042573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HEY0101362.1"
                     /translation="PEAQRFSPIELEYENSDKRKYFVPCPHCGEYQALEWKRNGEYTI
                     RWDGDDTANAYYVCVSGCVIEHEHKPEMLARGEWRAEKPCSGRAGFHIWEGYSPFVTW
                     GEMVKNFLEAKKSRSTLKVFVNTSLAEGWEEITEQASTDDLIDRCEEFAVAVPRGVLV
                     LTAGVDVQGNRLEVEIVGWGLDEESWSIGYHVIEGDPAQSQVWEDLKELLSRDYEFEV
                     PVGGESDDDAAQIVTTRVRATCIDSGGHHTQHVYRFCRQNKGRRVYAIKGANTPGKPL
                     ISKFTLQGKPRVRLYTVGTETAKDTIAAHLLITDPGPGYCHFPVTRDENYFKQLRSER
                     PVMRLSGGKTFRRWEKIKASARNEALDLRVYAMAARAILNPNFRAIARRTRAAAEAVA
                     ATQEATAASTPPETQRAGQEAEDVQTSAPDEQGQARPPRRRARRRGQRNFATDWR"
     gene            1348..1923
                     /locus_tag="VGB76_20735"
     CDS             1348..1923
                     /locus_tag="VGB76_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101363.1"
                     /translation="MAAELRSYEPREIATGETPQWTKSFDDYPASDGWALNYYFRGPG
                     AGFDVAATADGDDFIAAVPAGATDALTPGAYFWQAWVSKDTEKYQVGDGAVTVKQGFA
                     AVTTAMTVDNRSKAKRIIDAIDATLEGRATTDQHMYMIGNRQLMRIPVEQLISLRGHY
                     ATIYGEELRQARARRGGTLFKTLKVRFTKPK"
     gene            1920..3473
                     /locus_tag="VGB76_20740"
     CDS             1920..3473
                     /locus_tag="VGB76_20740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEY0101364.1"
                     /translation="MSILNLQLPTFSEIREEQKEQAAAEERRHKKRLRRSYEAARRNR
                     LTNDWTTVNTSANWELRRSLRVLRARSRNLARNNDYVKKFLSMVRTNIAGPHGMKMQA
                     RATNAKGEFDELLNRKVEAAWKRWSHKENASASGKLSWVDIQRKLCTMLARDGEALVR
                     AIVADNPFGFALKFISADWLDETFNERLTNGNRVIMSVEIDVNDRPVAYWLTPPPSDY
                     QYLDNNVRARTRVPAEEIIHCYLPDDENADDDCQTRGVPWIHTAMHRLQILGAYEEAE
                     LVAARVGASKMGFYQKTTPDGEEGEYEGDDNDDGTAELMENAEAGTFGIIPQGYEFKE
                     FKPDHPNTGYGSFTKGVLRGLSAGLDVSYFTLAEDLEGVNYSSARIGLMSEREQWRAL
                     QQFLIEHFSRSVYLMWLKSAMLAGALDIRVLDYKRLLEPVFQPRGWAWVDPKTDALAT
                     VTGIDNGFDTITDTLAEQGKDIEEVLATRKRELKLLKDYGIELHPEKPRPDAPSDEEE
                     APPPKSPKS"
     gene            3532..5580
                     /locus_tag="VGB76_20745"
     CDS             3532..5580
                     /locus_tag="VGB76_20745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEY0101365.1"
                     /translation="MAMKQLQRAQLDKLLGKKLKRAMPLQRAEMTIDEEARTVEMSFS
                     SDEPIEHWFGRLILNHKPDCIRLDRLNNGGAFLSEHDRTKQIGVHVDGTVKTDGHKMR
                     GTVRFSRSPLGDQEFQDVIDGIRRHTSVGAMLHELHFVRETDEEGPVYQSDDWEPIED
                     SLVSVPADVTTGVGRALDDDEEALAADVREQREEKCACDGKGCDACKAEREQPTQDSG
                     AEGRANQQPTLERSAMEQDTEILQLGEALGETELARDFVANSTGDLAAFKRAVAEKRR
                     KSQTPTPTEDPAQSAERNGGESVQIARGAYTGGLKSFRGKDAAKTAYRFGMFMIAGPM
                     ARAAGSKLHKRAMDFCREQGILLERAHSESDNESGGFLVPTEFEAVMIDLRELFGVFR
                     RFANVVPMASDSKTRPRRKGGLLAKPVGAKGASRRGTESKKKWDLVELFARKWMVLAK
                     YEEELSEDAVISMADDLAYEIAYAFSVSEDECGFIGDGSGDYHGIVGLTKRLRNPNNA
                     AVVPTLGHVASLVEASDDTWDGITRADFLKVIGRLPQYADGPNARWFCSKTFWAEVMV
                     RIILEAGGVTAAEVEGARVKSFMGYQVEVAQVLPKSSAVSQIPVVLGDLRKSSMFGDR
                     RGITIKLTDSNDTDFEEDLMSIKGTQRFDINNHDVGNVAASADDREPGPVIGLITKAA
                     "
     gene            5656..6096
                     /locus_tag="VGB76_20750"
     CDS             5656..6096
                     /locus_tag="VGB76_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101366.1"
                     /translation="MINSLAQKVVAVTNPGAIVDNAAFTTATIDTLGFRFVTIIVLFG
                     ALDIALAALKLREGDAADMSDATDVEGADFSVAPATLPAATDDNKLVAIHVNLNGRKR
                     YLDLTMTGGDGAAGTYASVTAILSRASEAPDTAEKRGFAQELIV"
     gene            6226..6525
                     /locus_tag="VGB76_20755"
     CDS             6226..6525
                     /locus_tag="VGB76_20755"
                     /inference="COORDINATES: protein motif:HMM:NF017192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HEY0101367.1"
                     /translation="MAFTEDLSLFFDVTKGFAVPAVFTRGEVEIATASVIFNDPSQAA
                     EIYGTPIEAAAPFLQAPTASLVALKRGDTATVNDATYRVEIIRPDGTGVSNVFLA"
     gene            6528..6995
                     /locus_tag="VGB76_20760"
     CDS             6528..6995
                     /locus_tag="VGB76_20760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101368.1"
                     /translation="MADTKRQKIVSAFIARMQSILTENDYQTDIGARVEDWPRRFAEA
                     DLAEQPSKAVIGIYDLPDDITKENKSSIRVTHRLRMQARIFITSATAPEDLRKMIGDV
                     VKAIGVDVTWGDLATDTEPASEGFIVPQDSMEVAGAAVECVIEYLTDTFDPYL"
     gene            7010..7777
                     /locus_tag="VGB76_20765"
     CDS             7010..7777
                     /locus_tag="VGB76_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101369.1"
                     /translation="MATEYYLGKGKLQIADRDANGAPGALTDVGEVPLISIEVTKDYA
                     ENFATSGEENVQDAHVPTKRTVKASITLKEASAKNLELILHGKKTANSGGSVTNQAFP
                     SGIVAGETHRLPGFAGIASSIAITDSAGSPVTLVEGTNYTVDADYGDVKFILVTGFTQ
                     PFKAAFTNAASTRVSILSKRVSNKFLRFKGINIGNNSGKKKMLAELYDCSLMPAKKVD
                     LKGDEHATYEVEIVLLADSTKAESEELGRYGNVLLLD"
     gene            7860..8243
                     /locus_tag="VGB76_20770"
     CDS             7860..8243
                     /locus_tag="VGB76_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101370.1"
                     /translation="MSQTQKYDAGLLSASAPHKATAKLTIFNQQGEKETVETDVFYRG
                     VSLDETEEFPDLEGLEGKARAAAIAKQLAFLVLSIPIFGVGVGAEFPVKPDEKYFGSM
                     DTAHVDAISTAIEESTSPNTSASNS"
BASE COUNT         1791 a         2614 c         2600 g         1365 t
ORIGIN      
        1 tccggaggca cagcgtttct cgcccatcga actcgaatac gagaactccg ataagcgcaa
       61 atacttcgtg ccgtgcccgc actgcggcga gtatcaggcg cttgaatgga agcgcaacgg
      121 cgaatacacc attaggtggg acggcgacga cacggcaaac gcttactacg tctgtgtctc
      181 cgggtgtgtc atcgagcacg aacacaagcc tgagatgctc gcgcgcggtg aatggcgcgc
      241 ggagaaaccc tgcagcggtc gcgccggctt tcacatctgg gaaggctact cgccatttgt
      301 cacctggggc gagatggtga agaacttcct cgaagcgaag aagagccgct ccacacttaa
      361 agtcttcgtt aacacgtcgc tcgccgaggg ctgggaagag atcacggagc aggcgagcac
      421 ggacgatctg attgatcggt gcgaggagtt cgcagtggcc gtgccgcgcg gcgtgctggt
      481 gctgaccgca ggcgtggacg tacagggcaa ccgtctggag gtggagatcg tcggctgggg
      541 cctcgacgag gagtcatggt caatcggcta ccacgttatt gaaggcgacc cggcccagtc
      601 gcaggtctgg gaagatctga aggaactgct gtcacgcgat tacgagttcg aggtgcctgt
      661 cggcggggag tcggacgatg acgcggcgca gatcgtgacg acgcgcgtgc gcgcgacctg
      721 catcgacagc ggcgggcacc acacgcagca cgtctatcgc ttctgccgtc agaacaaggg
      781 gcggcgcgtc tacgccatta agggcgcgaa cacgccgggc aagccactca tctcgaagtt
      841 caccttgcag ggcaaaccgc gtgtgcgcct ctacaccgtc ggcacggaga cggcgaagga
      901 caccatcgcc gcgcacctgc tcatcacgga tcccggcccg ggctactgcc acttccccgt
      961 cacccgcgac gagaactact tcaagcaact ccggagcgag cggcccgtga tgcgcctgag
     1021 cggcggcaag accttccgcc ggtgggagaa gatcaaggca tcggcgcgca acgaggcgct
     1081 cgacctgcgc gtctacgcga tggccgcgcg cgccatcctc aaccccaatt tccgcgccat
     1141 cgcccgtcgc acgcgcgccg cggcggaagc ggtcgccgcg acgcaggaag cgacggctgc
     1201 cagcacaccg cccgagactc agcgcgcggg ccaagaagcc gaggatgtac agacgtctgc
     1261 acctgacgaa caagggcagg ctcgaccgcc gcgccggcga gcacgtcgcc gcgggcagag
     1321 aaacttcgcc actgactgga gatgatgatg gcagcagagc tacgcagtta tgaaccgcgc
     1381 gagatcgcca ccggcgagac gccgcaatgg acgaagagct tcgacgacta cccggcatcg
     1441 gacgggtggg cgctcaatta ttactttcgc gggcccggcg cgggcttcga cgtcgcggcg
     1501 actgccgacg gtgatgactt catcgccgcg gtgccggcgg gcgcaaccga cgctctcacg
     1561 ccgggcgcct atttctggca ggcgtgggtg agcaaagata ctgagaagta tcaggtcggc
     1621 gacggcgccg tcacggtcaa gcagggcttc gccgccgtca cgaccgcgat gactgttgat
     1681 aaccggtcga aggccaagcg gatcatcgac gctatcgacg ctacgttgga ggggcgtgcg
     1741 acaaccgacc agcacatgta catgatcggc aaccgccagc tcatgcgcat tcccgtcgaa
     1801 caactcatct ccctgcgcgg gcattacgcg accatctacg gcgaggagct gcgacaggct
     1861 cgggcgcgcc gcggcggcac tctcttcaaa acccttaaag tgaggttcac taaaccgaaa
     1921 tgagtatctt aaatctccag ttgccgacct tctccgagat ccgggaagag caaaaagaac
     1981 aggccgccgc cgaggaacgc cgccacaaga aacggctcag gcgctcctac gaagccgcgc
     2041 gccgcaaccg gttgaccaac gactggacga ccgtcaacac gtcggccaac tgggagttgc
     2101 gccggagcct ccgcgtgctc cgcgcccgct cgcgcaacct ggcgcgcaac aacgactacg
     2161 tcaaaaagtt tctctcgatg gtacgcacga acatcgccgg gccgcacggc atgaagatgc
     2221 aggcgcgggc gacgaacgcg aaaggcgagt tcgacgagtt gttgaaccgt aaggtcgagg
     2281 cggcgtggaa aagatggagc cacaaggaga acgcttcggc ctcgggcaaa ctttcatggg
     2341 tggacatcca gcggaagcta tgcacgatgc tcgcgcgcga tggcgaagcg ttggttcgcg
     2401 ccatcgtcgc cgacaacccg ttcggcttcg cattgaagtt catcagcgcg gactggcttg
     2461 acgagacgtt caacgagcgg ctcacgaacg gtaaccgggt catcatgtcg gtggagattg
     2521 acgtgaatga ccgcccggtc gcctactggc tgacaccacc gccgtcggat taccagtatc
     2581 tcgacaacaa cgtcagggcg cgcacgcgcg tgccagccga agagatcatc cattgctatc
     2641 tgccggatga cgagaacgct gacgacgact gccagacgcg cggcgtgccg tggatccaca
     2701 cggccatgca ccggctgcag atactgggcg cttatgagga ggcggaactc gtggctgcgc
     2761 gggtcggcgc gtcgaagatg ggcttctatc agaaaacaac gcccgacggg gaagaagggg
     2821 agtatgaggg cgatgacaat gacgacggca ccgccgagtt gatggagaac gcggaggccg
     2881 gcacgttcgg catcatcccg caaggctatg agttcaagga gttcaagccg gaccacccga
     2941 acacaggcta tggctcattc accaagggcg tgctgcgcgg cctctccgcc ggcctcgacg
     3001 tgtcctactt caccctggcc gaagacctcg aaggcgtcaa ctactcgtcc gcccgcatcg
     3061 gcctgatgtc ggaacgggag cagtggcggg cgctgcaaca atttttaatc gagcacttta
     3121 gccggagcgt gtacctcatg tggctgaagt ccgcgatgct ggccggcgca cttgatattc
     3181 gcgtgctgga ttacaagcgc ctgttggagc cggtcttcca gccgcgtggc tgggcgtggg
     3241 tcgatccgaa gaccgatgcg ctggccacag tcaccggcat cgacaacggc ttcgacacca
     3301 tcactgacac gctggccgag cagggcaaag acatcgagga ggttctcgcc acccgcaagc
     3361 gtgagttgaa gctgctgaag gactacggca tcgaattaca tcctgaaaaa ccccgcccgg
     3421 acgccccgtc cgacgaggag gaagcgcccc cgccgaagtc gccaaaatca taaaatttca
     3481 attctgaata tagttgccgg tgtgagagga cagcactctc cgccggcaac tatggcaatg
     3541 aagcaactcc aacgcgcgca actcgacaaa ctgctcggca aaaagctgaa gcgggcgatg
     3601 cccctccagc gcgccgagat gaccattgac gaggaagcgc gcacggtcga gatgtcattc
     3661 tcttccgacg agcccattga gcactggttc ggcaggctca tcctcaacca caagcccgat
     3721 tgcatccgcc tcgaccgcct gaacaacggc ggcgcctttc tctccgagca cgaccgcacc
     3781 aaacagatcg gcgtccacgt agacggcacc gtcaagaccg acggacacaa gatgcgcggc
     3841 accgtccgct tcagccgctc cccgctcggc gaccaggagt tccaagacgt cattgacggc
     3901 atccgccgtc atacgtccgt cggggcgatg cttcacgaac tccactttgt gcgcgagacg
     3961 gacgaggagg ggccggtcta ccagtcagac gactgggagc cgattgagga ctcgctcgtc
     4021 tctgtcccgg cggatgtcac cacaggcgtc ggacgtgcgc tcgacgatga cgaggaggca
     4081 ctggccgcgg acgttcgtga gcagcgggaa gagaagtgcg cgtgtgacgg caaaggctgc
     4141 gatgcctgca aggcggagcg tgaacaaccc acccaagatt ctggcgccga agggcgtgcc
     4201 aatcaacaac ccactttaga gaggtcagca atggaacagg acacagagat tcttcaactc
     4261 ggcgaagcgc tgggtgaaac ggaattggcg cgcgacttcg tcgccaacag caccggcgac
     4321 ttagccgcgt tcaagcgcgc cgtcgccgag aagcgcagga agtcgcagac gcccacgccg
     4381 acggaagacc cggcgcagtc cgccgagcgc aacggcggcg aaagcgtgca gatcgcgcgc
     4441 ggcgcctaca ccggcggcct caagagcttc cgcggcaagg atgcagccaa gaccgcctat
     4501 cgcttcggca tgttcatgat cgccggcccg atggctcgcg cggctggttc caagttgcac
     4561 aagcgcgcga tggacttctg ccgtgagcag ggcatcctct tggagcgcgc gcacagtgag
     4621 agcgacaacg agtcgggcgg cttcctggtg ccgacggaat ttgaggcggt gatgatcgac
     4681 ctgcgcgagc tgttcggcgt cttccgccgc ttcgccaacg tcgtgccgat ggcttcggac
     4741 tcgaagacgc gtccgcgccg caagggcggc ctcctggcca agcccgtcgg cgcgaagggc
     4801 gctagccgtc gcggcaccga gtcgaagaag aagtgggact tggtcgaact gttcgcccgc
     4861 aagtggatgg tgctcgccaa gtacgaagag gagctttccg aagacgcggt catctcgatg
     4921 gccgacgatc tggcctacga gattgcctac gccttctcgg tgagcgagga cgagtgcggc
     4981 tttatcggcg acggctctgg ggactatcac ggcatcgttg gcctaaccaa gcgcttgcgt
     5041 aacccaaaca acgctgcggt cgtgccgacg ctcggccacg tcgcgagtct cgtcgaggcg
     5101 tcggacgata cctgggacgg gatcacgcgc gccgacttcc tgaaagtcat cgggcgtctc
     5161 ccgcagtatg ccgacgggcc gaacgcccgc tggttctgct cgaagacgtt ctgggctgaa
     5221 gtgatggtgc gcatcatcct cgaagccggc ggcgtaaccg ctgccgaggt cgagggcgcg
     5281 cgtgtcaagt ccttcatggg ctaccaagtc gaggtcgcgc aggtgctgcc gaagtcttcc
     5341 gccgtgagtc agatcccggt cgtcctcggc gacctgcgca agtcctcgat gttcggcgac
     5401 cgccgcggca tcacgatcaa gctcacggac tcgaacgaca cggacttcga ggaagacctc
     5461 atgtccatca agggcacgca gcgcttcgac atcaacaacc acgatgtggg caacgtcgcg
     5521 gcgtcggctg acgaccgcga gcccggcccc gtcatcggct tgatcacgaa ggccgcgtag
     5581 ccgtcaccgt cgtctcgaaa cctgaatccg gcgggaggct ctccccctcc gcccacaact
     5641 tagaggtcaa ttgaaatgat taacagtctt gctcagaaag tcgtcgccgt caccaatccg
     5701 ggtgcgattg tggataacgc ggccttcacc actgccacca tcgacacgct tggctttcgc
     5761 ttcgtgacga tcatcgtctt gttcggcgcg ctcgacatcg cgctcgccgc actgaaactg
     5821 cgcgagggcg acgcagcgga catgtcggac gcgacggatg tggagggcgc ggacttcagt
     5881 gttgcgccgg ccacgctgcc ggcggccacg gacgacaata agctcgttgc catccacgtc
     5941 aacctgaacg gtaggaaacg ctacctcgat ctgacgatga cgggtggcga cggcgcggcg
     6001 ggcacttacg cctccgtcac tgcgattttg tcgcgcgcct cggaagctcc ggacaccgcg
     6061 gagaaacgcg gcttcgcgca ggagttgatc gtctgacgac tttccgggct ggagcctgaa
     6121 ccggcgagca gcgctgcgag actcgcgtca cgacaccgac cgagccagta ctgccttcga
     6181 cgctcccgct ccggcccgga ataacttttt cgagaggtaa ctatcttggc attcaccgaa
     6241 gacctctcac tgttctttga cgtaaccaaa ggcttcgcgg ttcctgccgt gttcacacgc
     6301 ggcgaggtag agatcgcaac tgcctcggtc atctttaacg acccctcgca ggctgccgaa
     6361 atatatggca cgcctatcga ggcggcggcg ccgttcctgc aggctccgac cgcgtcgctc
     6421 gtcgctctaa agcgcggcga cacggcgaca gtgaacgacg caacttatcg cgtcgagatc
     6481 atccgccccg acggcacggg cgtctcaaac gtctttctgg cgtaaccatg gcagacacga
     6541 aacgacaaaa gattgtgtcc gccttcatcg cccggatgca gagcatcctg acggagaacg
     6601 attaccagac cgacatcggg gcgcgcgtgg aggactggcc gcgcaggttc gctgaggctg
     6661 acctagcaga gcaaccaagc aaggcagtca ttggcattta cgacctgccc gatgacatca
     6721 cgaaggagaa caaaagttcc atccgcgtga ctcaccgcct gcgcatgcaa gcgcgcatct
     6781 tcatcacaag cgcgacggct cccgaagact tgcgaaagat gatcggcgat gtcgtgaaag
     6841 ctattggcgt ggatgtgacc tggggtgatc tggcaactga cacggagccg gcttctgaag
     6901 gcttcatcgt gccgcaggat tcaatggaag tggcgggcgc cgccgtcgag tgcgtcatcg
     6961 agtatttgac cgacacgttt gacccgtacc tgtaggagag gaaaaggaca tggcaactga
     7021 atattatctc ggcaaaggca aactacagat cgccgaccgc gacgccaacg gtgcgcccgg
     7081 cgctctcacc gacgtgggcg aagtgccgct catcagcatc gaggtcacaa aagactatgc
     7141 cgagaacttc gccacgagcg gcgaggagaa cgtgcaggac gcccacgtgc ccaccaagcg
     7201 cacggtcaag gcgagcatca cgctcaagga agcgtcagcc aagaacctcg aactcattct
     7261 ccatggcaag aagaccgcga actcgggcgg cagtgtgacg aaccaggcgt tcccgtccgg
     7321 catcgtcgcg ggcgagacgc accggctgcc gggcttcgcc ggcattgcct cgtccatcgc
     7381 tatcacagac agcgccggca gtccggtcac gctcgtcgaa gggactaact acacggtcga
     7441 tgctgattac ggtgacgtca aattcatcct cgtgacgggc ttcacgcagc ccttcaaggc
     7501 ggccttcacc aacgcggctt cgacccgcgt ctcgatcctg tcgaagcgcg tgtccaacaa
     7561 gttcctgcgc ttcaagggca tcaacatcgg caacaacagc ggcaagaaaa agatgctcgc
     7621 tgagttgtat gactgctcgc tgatgcccgc gaagaaggtt gatctgaagg gcgacgagca
     7681 cgcgacctac gaagtggaga tcgtcctcct ggccgactcg acgaaggccg agagtgagga
     7741 actgggtcgc tacggtaacg tgctcttact cgactaacta catcggcatt tcaacaacca
     7801 cgtgcgcggg agccatgccg ataaggtatc ccgcgcattt tctcaaggag ccaactgaaa
     7861 tgagtcaaac gcaaaaatat gacgccggtc tgctgagcgc gagcgcgccg cacaaggcca
     7921 cggccaagct aaccatcttc aaccagcagg gcgagaagga aacggtcgag acggacgtct
     7981 tctaccgcgg ggtgtcgctc gacgagacag aagagtttcc ggacttggaa ggcttggaag
     8041 gtaaagcgcg cgctgcggcc atcgcgaagc aactcgcctt cctcgtgctg agcatcccga
     8101 tcttcggcgt gggcgtcggt gcggagttcc cggtcaagcc ggacgagaag tatttcggct
     8161 ccatggacac cgcgcacgtg gacgccatca gcacggcaat cgaggagagc acctccccaa
     8221 acacgtcagc ctccaactcc tagccgcctg gtacaagtct ggaggcaaag ccggcgagcc
     8281 gctgcccgag cagaacatca tcgcgctggc cttcgcactc gggaagatgc ccgacgagat
     8341 agtcggttgt gacacctact ggatcaaccg
//
LOCUS       DASWNS010000606         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_385615, whole genome shotgun sequence.
ACCESSION   DASWNS010000606 DASWNS010000000
VERSION     DASWNS010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385615"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="VGB76_20775"
     CDS             <1..1327
                     /locus_tag="VGB76_20775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY0101371.1"
                     /translation="RAFQAAHKSYDVIVDALFGTGLTRPLEGIYASVIEHLAQVRKAR
                     GEESDAYPLIVSLDLPSGLNADAAELFGDAVQADLTVTFTAPKPANVLPPASYSNGKL
                     RVASIGSPAALIDQADSQLFLAERTDARAWLEQTRYRPGSYKNTHGHALVIAGSRRMS
                     GAAVLCGNAAMRAGAGLVTIAAPASALAAIAARVMPEVMTAELTETTEASAQETETGT
                     GLEPRVLSETAISQALQLAERADAIAIGPGLTSGEHIRRLVRTIVKRRTTPLIIDADG
                     LNALAPWPADLRGTPARPLILTPHPGEMRRLTGSHAVEDSQAERVRLARDFAVAHQLF
                     LVLKGSRVLIAAPDGRVAINPTGNAGTGTAGAGDTLTGIIIGFIAQARGTLKDAADDF
                     DTIVAAVYVAGLASDLAARGTGMRAMVASDIGKYLGAAFRTLDPAGERP"
     gene            1324..1797
                     /gene="tsaE"
                     /locus_tag="VGB76_20780"
     CDS             1324..1797
                     /gene="tsaE"
                     /locus_tag="VGB76_20780"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY0101372.1"
                     /translation="MTTTPDFDSTSAFAPAGEWTSHTPAETFGFGEWIGKHLTGGEII
                     LLDGALGAGKTIFVKGLCSALGLDEHEVTSPSFTLVNRYEARLTLYHLDLYRLPDGTS
                     AAYAVDLDELLMDERAVIVIEWAERLRNYPLPAPIYRVNIEGDGEEVRRIRIVKD"
     gene            1885..2325
                     /locus_tag="VGB76_20785"
     CDS             1885..2325
                     /locus_tag="VGB76_20785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEY0101373.1"
                     /translation="MPYPEIMIRPMREELTRLGIEEARTPEQVEEAIKQSEGTVMVVV
                     NSVCGCAAGKARPGIARAMQHSTRPDRAITVFAGADIEATEKARDYFTGHQPSSPSIA
                     LLKNGELVYIMERYQIEGKDAPTIAQELARAFDQHCSTTSAANA"
BASE COUNT          494 a          817 c          678 g          417 t
ORIGIN      
        1 gcgcgcgttt caggcggcgc acaaaagcta cgacgtgatc gtcgatgcgt tgttcggcac
       61 cggactcaca cgaccgctcg aaggcattta cgcttccgtc atcgagcacc tcgcgcaggt
      121 gcgaaaagct cgcggcgaag agagcgacgc ttatccgctg atcgtctcgc tcgatctgcc
      181 gtccggcctc aacgccgatg ccgccgagtt gttcggcgac gccgtgcagg ccgatctcac
      241 agtcaccttc accgcgccca aaccggccaa cgtcctgccg ccggcctctt actccaacgg
      301 caaattgcgc gtcgcatcca tcggctctcc cgccgctctc attgaccagg ccgactcgca
      361 acttttcctc gccgaacgaa ccgacgcacg cgcgtggctc gaacagacgc gctaccgccc
      421 cggctcctac aaaaatacgc acggccacgc cctcgtcatc gcaggctcgc ggcggatgtc
      481 cggcgcggca gtgctctgcg gcaacgcggc catgcgcgca ggcgcgggac tcgtcacaat
      541 cgccgcaccc gcatccgcgc tggccgccat cgccgcgcgc gtcatgcccg aagtgatgac
      601 cgccgagtta acagagacga cggaagcctc ggctcaagag actgaaaccg gaacaggctt
      661 ggagccgcgc gtcttgagcg agacggccat ctcacaggct ttacaactcg cggagcgcgc
      721 cgacgctatc gccatcggcc ccggtttaac gtccggcgag cacatccgac gcctcgtgcg
      781 aaccatcgtc aaacggcgca caactccgct cattattgat gccgacgggc tgaacgcgct
      841 cgccccgtgg cctgccgact tgcgcggcac gcccgcacgc ccgctcatcc tcacgccgca
      901 ccccggcgag atgcggcggc tcacaggctc acacgccgtc gaagattcac aagctgaacg
      961 cgtccggctc gccagagact ttgccgtggc tcatcaactc tttctcgtct taaaaggatc
     1021 gcgcgtgctc atcgccgcgc ccgatggacg cgtcgcgatc aacccgaccg gcaatgccgg
     1081 aacgggcacg gcgggcgcgg gcgacacgtt gacgggcatc atcataggct tcatcgcaca
     1141 agctcgcggc acgctcaaag atgcggcgga tgacttcgac acaatagtcg ccgccgtcta
     1201 tgtcgcggga cttgcgagcg acctcgccgc acgaggcaca ggtatgcgcg caatggtcgc
     1261 ctccgacatc ggcaagtatc tcggcgcagc ctttcgcaca ctcgaccccg caggtgaacg
     1321 accatgacga ctactcctga tttcgattca acttccgctt ttgctccggc gggcgaatgg
     1381 acttcgcaca caccggcgga gacgttcggg ttcggcgagt ggatcggaaa acatttgacc
     1441 ggcggcgaga tcattttgct cgacggcgct ttgggcgcgg gcaagacgat cttcgtgaaa
     1501 ggtctgtgca gcgcgctcgg actcgatgaa catgaagtga cgagtccatc cttcaccctc
     1561 gtcaaccgct atgaggcgcg cttgacgctc taccatctcg acctgtaccg cctgcccgat
     1621 gggacatcgg cggcctacgc cgtcgatctc gacgaactac tgatggatga acgcgccgtc
     1681 atcgtcattg aatgggcgga acgccttcgg aactacccgt tgcccgcgcc catctatcgc
     1741 gttaacatcg aaggcgacgg tgaagaagtt cggcgtatcc gcatcgttaa ggactgacgc
     1801 tgcacagtcg tcgcgccccg tgtaaatcgc gcggctatgt tagaatttgc gggagacaat
     1861 ttatcagcag aggagaaatt agttatgcct tatccagaga ttatgattcg cccgatgcgc
     1921 gaggaactga cgcgactcgg cattgaagaa gcacgcacgc ccgaacaggt tgaagaagcg
     1981 atcaagcagt cggaaggtac tgtcatggtt gtcgtcaatt ccgtctgcgg ctgcgcggcg
     2041 ggcaaagcgc gccccggcat cgcccgcgcc atgcaacaca gcacgcgccc tgaccgtgcc
     2101 atcaccgtct ttgccggagc cgacatcgaa gccacagaga aggcgcgcga ctatttcacg
     2161 ggacatcaac cgtcgtcgcc ctccatcgcc ctgctcaaaa acggcgaact cgtctacatc
     2221 atggagcgtt accagatcga gggcaaagac gcgcccacca tcgcgcagga actcgcccgc
     2281 gccttcgacc agcactgctc caccaccagc gccgccaatg cttaactaca gtgacaagtg
     2341 acaagcgaca agtaacgagt gacgagttaa gaccgatgct ttaactcgtc actcgccact
     2401 tgtcac
//
LOCUS       DASWNS010000607        10136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_153792, whole genome shotgun sequence.
ACCESSION   DASWNS010000607 DASWNS010000000
VERSION     DASWNS010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153792"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(533..1675)
                     /locus_tag="VGB76_20790"
     CDS             complement(533..1675)
                     /locus_tag="VGB76_20790"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY0101374.1"
                     /translation="MPPTKKSIGFRELRVGIFVIVALAVLIFLILQASGDISFARKIR
                     LKAQFLTAEGLRPGGEVRLAGVRIGKVEDVRLLPPSDNPQAMKVEAVLALDSKVDGKS
                     VTDLVRTDSKAQLATPSLLGSDRIINITPGTSLGNPVQEGQSLPSATEGGMTQLTESG
                     NQLVGQLNQLSQQVTDITRKINEGQGTLGRFVNDEAFYDNLNVTIRETQGIMRQLRSG
                     DGTAGKLINDPALYNNLNAVTAQLQGISEDLRAGRGTAGKLLTDEALYNDARTTIARL
                     NTSVEEITKITTDIRQGRGTAGKLLTDEALYNDARAAIARFNTSAERIDNIITGIQRG
                     EGTAGKLLTDEALYNNVNQLSSETVKLMYDFRQNPKKYLTIKFSIF"
     gene            complement(1789..2709)
                     /locus_tag="VGB76_20795"
     CDS             complement(1789..2709)
                     /locus_tag="VGB76_20795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0101375.1"
                     /translation="MASTDIEISPAPDATPLEGRPVSAAASATTFAAEDDNERVTPAI
                     EFRNVHLSFDDRKVLDGLSFKVAKGETKIILGGSGCGKSTTIKLVLGLLKPDEGQILV
                     NGEDITHYTEAQMMRVRKNFGMIFQEGALFDSLSVYENVAYRLHEQGVPEDEVEQEVR
                     RMLRFVNLEDAIDKMPAELSGGMRRRVGIARALVGGPTIVMFDEPTAGLDPPTARTIC
                     ELAIKLRDLEDVSSIFVTHEMNNVEYLTREYAAVNDAGEVIFEQEGERLCLINTKLLM
                     MKEGNIIFSGTDEMLKRNEDPYIKRFLRGH"
     gene            complement(2727..3497)
                     /locus_tag="VGB76_20800"
     CDS             complement(2727..3497)
                     /locus_tag="VGB76_20800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0101376.1"
                     /translation="MNIALRSLLEVQETSLLVARAARRLFRRPTYFNEMIAQMDVIGV
                     GSLTIIVLTGFFTGGVLTLQTFPTLKYYGAQDQTGYLVALSLVRELGPVLSALMVTGR
                     VGSAISAELGSMVVSQQIDAMRALGTDPVRKLVAPRLVALFITLPLLTVAADVVGIIG
                     GGVVATTLYNQSAGMFLSSVRYGITFDDIIGGVIKPLVFALIIGSISCYKGLSTQGGT
                     VGVGRSTTSAVVTSSIVVIIADFFLAKALQVILDMPGT"
     gene            complement(3909..4505)
                     /locus_tag="VGB76_20805"
     CDS             complement(3909..4505)
                     /locus_tag="VGB76_20805"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEY0101377.1"
                     /translation="MRESRNNFSSRRFFVLAHALLALLLLAGSGVAVRAQDGDEPETA
                     SEARRGVGAGGLLRALNLTPEQRAQINAIRREIEPQGRLLGMRLRRARRALDEAIYAD
                     NPDDGVIEERVRELGAAQSAVSRMRAFTELKIRRVLSPEQLDAFRQLQRETRPRQRQR
                     RQQRQQRRNQTSPQDDFPGGLAPEPLRNRRQRRGRAQQ"
     gene            complement(4563..5366)
                     /locus_tag="VGB76_20810"
     CDS             complement(4563..5366)
                     /locus_tag="VGB76_20810"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0101378.1"
                     /translation="MNETTNTNCCERAGELVAYFYGEATRDERESFDAHLPQCDACRD
                     ELIAFAGVREAIGDWRAEILHAAPTAPSFAGTVATATQGARGESVRAASPAPRGASSA
                     LAALREFFTLSPMWMRAGMAAAALVVCALAALAIVNAEVRREEGGGITFRTRLRSGAA
                     QATTQVAAPAAKVYTQAELDSLVAERDAALRELEDTRAQLDDSRAANLIAAVETLEPV
                     PASSMEAPAASNSRRARRLSPSNRRQPRLRSDRDEDDVPRLYDLLIEAN"
     gene            complement(5391..5978)
                     /locus_tag="VGB76_20815"
     CDS             complement(5391..5978)
                     /locus_tag="VGB76_20815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0101379.1"
                     /translation="MATAETSDEQIVERALAGDVDAFGEIVQRWERRIYALAYGILGR
                     EEEAHDATQETFIAAFRNLRGFRGEAKVSSWLHRIAVNQCITRQRRARTRAENSLETE
                     TELGGEKFAAPAHASPARQVEGRERTAAVRRAVMSLPPELREVIVMKEFEELTFQEIA
                     TTLDVPLSTVKSRLYTALKQLRLRLEKHGAQAAHV"
     gene            6328..8172
                     /locus_tag="VGB76_20820"
     CDS             6328..8172
                     /locus_tag="VGB76_20820"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0101380.1"
                     /translation="MLERFRRVRLLYIVLGTLLFVGLVPLGLAGWMLSNRSANELRSI
                     EGRYQAQLVQDKARQIELYGQRYRDVVTGLARAFELAGGVRVIGEEGSDARLQKSVVD
                     DPNLLALAILPVGSLPHVAYKPDVIGREELNERVGEVLARMSGRGLVVTRPQLIRSSQ
                     EMSLTIAAPVMGGANGEEVVAAVAAVISFNEVFAAVQQPTSKSERELLEQGLPVIFVV
                     DPSGRAVAHPDQRVAYAERSMSDLLIVQNWMDTGTQVESLLEPFTAERDGRQIAMLGA
                     YATAKLDTGARLGVIAIQDERAALRSVADMRKQTFMISLLAATFALLIGFLFAKQLTQ
                     PVRDLADAAGRIANGNFAQRIQVRSRTELGELGTSFNLMTDQLEQYIEDLRSAAEENR
                     QLFVGTVKALAAAIDGKDRYTRGHSERVARFSIAIAENLGLPEGEIEALRISALLHDV
                     GKIGIDDKVLKKPAKLTDEEFEIMKTHPQKGFKIMSNIPAMREFLPGMYMHHEMINGE
                     GYPQGLKGDEIPMQARIVSVADTFDAMTTDRPYQKAMELEDALARIKTFVGSRYDERV
                     VDALVEACESGRINAVGTTRLRPRRLPERVAGNVPPAVEPPVEQTQTL"
     gene            complement(8245..8643)
                     /locus_tag="VGB76_20825"
     CDS             complement(8245..8643)
                     /locus_tag="VGB76_20825"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0101381.1"
                     /translation="MPVLDNDDKRQVRQLELDLIEAFRRNDTASLARILADDFVFTDP
                     RGASVTKDLWLEDLASGTFAFESVEIEDIRVRLAGDAALVTGLATLKARSRAGSYNGR
                     FGVVDVYMKREGRWQLALSTGEHAQLLSYE"
     gene            8785..>10136
                     /locus_tag="VGB76_20830"
     CDS             8785..>10136
                     /locus_tag="VGB76_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101382.1"
                     /translation="MLRKLSVLSICLWLVLPALAQQPTPSPSPDKRTEQLEDALRKAE
                     AEKRADKWDVEGDHGPTTKVEFDTDEGTWMSCDVSPDGTRIVFDLLGDIYHMPSGGGR
                     AELLSGGVSWESQPRYSPDGKSIAFTSDRDGGDNIWLMDADGKNRRQLTKETLRLVNS
                     PAWTPDSQYVLVRKHFVDQRSLGAGEIWMYHTGGGGTGVQMTEKSSWTANVGEPVIDP
                     QKRFIYFVNSGDFDYNKNVYESIYWIERYDTERGRRGVFVRGAGGSVRPQISPDGKLL
                     AFIRRDRLNTVLYLREIESGREWPIYDGLTRDQQETWSVFGTYPGYGWTPDGKAIVIT
                     AKGKFVRVDAQTKAATPIPFTARVSQRVADAVRFPQKVFAERDTTRLHRWPKRHEDRI
                     IYSALGKIYVKQGTAAPRRLLNSNYLEYSPNFSRDGRQITFVTWSDAEKGAVWIADAD
                     G"
BASE COUNT         1936 a         3006 c         3163 g         2031 t
ORIGIN      
        1 tactaaaaga gtagaagagt tgattgcaat tactgagatt ctaaagaaca aggcgcattt
       61 tgtatccact gaattatttc tgacaacaga acctctttcg acttggttcc atcaataaca
      121 aaatcagcgg actcttttat aatggtattt acttgcctct cagtagagtg agaatcagtt
      181 tggtgaatct cctcgtgggt aatattttca actttcagtc tgctctcacg cacagcatca
      241 tcaatatcta taaatactat gacggagaat ggagggctta accaactccc gcagaaattc
      301 tattatctca tggtgtcgga tcccatcaat tatgagtgcc tgccccggca tggtcatgtg
      361 tggaagttat agctcttcca aagaaaagca gtgggttgag catcactact caactcgctg
      421 cttacaaaac cgtgtaaaaa cgtaaaacgc agtacggaac tctattacat agtacccaat
      481 tctattacac agtacgcaac tctattacac tgtcacaaca agttcttcca catcagaaga
      541 ttgaaaattt gattgtcagg tactttttgg ggttttggcg gaagtcgtac atgagtttga
      601 cggtttcgga ggatagttgg ttgacgttgt tgtagagagc ttcgtcggtg aggagtttgc
      661 cggcggtgcc ttcgccgcgt tggatgccgg tgatgatgtt gtcgatgcgt tcggcggagg
      721 tgttaaagcg ggcgatggcg gcgcgggcat cgttgtagag ggcttcgtcg gtgaggagtt
      781 tgccggcggt gccgcgtccc tggcggatgt ctgtggtgat cttggtgatc tcttcgacgg
      841 aggtgttgag gcgcgcgatg gtggtgcggg catcgttgta gagagcttcg tcggtgagga
      901 gtttgccggc ggtgccgcga ccggcgcgga ggtcttcgga gatgccctga agttgggcgg
      961 tgacggcgtt gaggttgttg tagagagccg gatcgttgat gagcttgccg gcggtgccgt
     1021 cgccgctgcg gagttgacgc atgatgcctt gcgtttcgcg gatggtgacg ttgaggttgt
     1081 cgtagaacgc ctcgtcgttg acgaagcgac cgagcgtgcc ctgcccttcg ttgatcttgc
     1141 gcgtgatgtc cgtgacttgc tgcgagagtt ggttgagttg gccgacgagt tggttgccgg
     1201 actctgtgag ttgcgtcatg ccgccctcgg tcgcactggg caggctctgg ccttcctgca
     1261 cggggttgcc gagcgacgtg ccgggcgtga tgttgatgat gcggtcgctg ccgaggaggc
     1321 tgggcgtggc gagttgggct ttcgagtcgg tgcggacgag atcggtgaca gacttgccgt
     1381 caactttact gtcgagcgcg aggacggctt cgactttcat ggcttgcggg ttgtcgctcg
     1441 gcggcagcag ccgcacgtct tcaactttgc cgatgcggac tccggcgagg cgcacttcgc
     1501 cgccggggcg caagccctcc gccgtcaaaa actgcgcctt gaggcgaatc ttgcgcgcaa
     1561 aagagatgtc gccggaggct tgcaggatca ggaagatcaa gaccgccagc gcgacgatga
     1621 cgaagatgcc gacgcgcaac tcgcgaaaac cgattgactt tttggttgga ggcatagcgt
     1681 tgagggatga gggatgaggg atgagggatg aacaggaaaa attcaagtca atagacttga
     1741 atgcttttct gcttcatcct tcatccttcg tccttcatcg ttttcctttc agtgtccgcg
     1801 caaaaatctc ttgatgtagg ggtcttcgtt ccgcttgagc atttcgtccg tgccgctgaa
     1861 gatgatgttg ccttccttca tcatcagcaa cttggtgttg atcaggcaga gacgttcgcc
     1921 ctcctgctcg aagatgactt cgcccgcatc gttgaccgcc gcgtattcgc gcgtcagata
     1981 ttcgacgttg ttcatctcgt gtgtgacgaa gatggaagag acgtcttcga ggtcgcgcag
     2041 tttgatggcg agttcgcaga tggtgcgggc ggtcggcgga tcgagtccgg cggtcggctc
     2101 gtcgaacatc acgatggttg gaccgccgac gagagcgcgc gcgatgccga cgcggcggcg
     2161 catgccgccc gaaagctccg ctggcatttt gtcaatcgca tcttcgaggt tgacgaacct
     2221 gagcatgcgc cgcacttcct gttccacctc atcttcgggc acgccctgtt cgtgcaggcg
     2281 ataggcgacg ttttcataga cggagagcga atcgaagagc gcgccttcct gaaagatcat
     2341 gccgaagttt ttgcgcacgc gcatcatctg ggcttcggtg tagtgcgtga tgtcttcgcc
     2401 gttgacgaga atttgtccct cgtcgggttt gagcaggccg agcacgagtt tgatcgtggt
     2461 ggacttgccg cagccggagc cgccgagaat gatcttggtt tcgcccttcg ccactttgaa
     2521 actgaggccg tcgagcactt tgcggtcgtc gaaggagaga tgcacgttgc gaaactcgat
     2581 ggcgggcgtg acgcgctcgt tatcgtcttc ggcggcaaaa gtggtggccg aggccgccgc
     2641 gctcacaggc cggccttcca gcggcgtcgc gtccggtgcg ggtgaaattt cgatgtcggt
     2701 ggaggccata gaataaaagc gtaaactcag gtgcccggca tgtccagaat aacctgtaat
     2761 gccttggcaa ggaagaagtc agcgatgata acgacgatgg acgaggtgac gaccgccgaa
     2821 gtggtcgaac gcccgacgcc caccgtcccg ccctgcgtgc tcaagccctt gtaacaggag
     2881 atgctcccga tgatgagcgc gaagacgagc ggcttgatga caccgccgat gatgtcgtcg
     2941 aaggtgatgc cgtagcgcac cgaactgaga aacattcccg ccgactggtt gtagagcgtg
     3001 gtggcgacca cgccgccgcc gatgatgccg accacgtctg cggcaaccgt caggagcggc
     3061 aatgtgatga agagcgcgac gagacgcggc gcgacgagtt tgcgcacggg gtctgtgccg
     3121 agcgcgcgca tcgcgtcgat ctgctgcgag acgaccatcg agccgagttc ggcggagatg
     3181 gccgagccga cgcgcccggt caccatcaaa gcgctcagga cggggccgag ttcgcgcacg
     3241 agcgaaagcg cgacgagata gccggtttga tcttgcgcgc cgtagtattt gagcgtgggg
     3301 aaggtttgca gggtcaagac gccgccggtg aaaaaacctg tgagcacgat gatcgtgagc
     3361 gagccgacgc cgatcacgtc catctgcgcg atcatctcgt tgaaataggt aggccggcgg
     3421 aaaagcctgc gtgcggcgcg cgccaccagc aaactcgtct cctgaacttc cagcagagag
     3481 cgtagagcaa tgttcatgtg tgaactgttg aaattcgctg taactgcgcc cggttgtcgg
     3541 aattgcctgc cgcaccggaa cgcgtttcaa taaaacctgc ttgaagcgcg ttgccgacaa
     3601 tctgtgcgtg acttgatttg aagggcgcgc agacagactt gcgagacgag catgaacgaa
     3661 caggccaatg atgcgcaatt agtaggcaag gcgtcaagcg acaattaaga cgataagtga
     3721 aaaggccaaa ggcgaaagtg gagactgaaa gctctgcggc ttcctgtctc cactttcgct
     3781 gtttgcgttt agcgttttgt gcggcggctg accgtcactg cggacgcgcc ccccgcacgg
     3841 gagcgctgcg tagctcgcgc ggctcggagg cgtctgcgga cgcccggcgc agcgagggcg
     3901 ggcggcgttt attgttgcgc gcgcccgcgt cgctgccgcc tgtttctgag cggttcgggg
     3961 gcaaggccgc cggggaagtc gtcctgcggc gaagtttgat tcctgcgctg ctgccgctgc
     4021 tgccgtcgct gccgctgtcg cgggcgcgtc tctctttgca gttgacgaaa ggcgtcgagt
     4081 tgttcgggcg agaggacgcg gcgaatcttc aactccgtga acgcacgcat gcgcgacact
     4141 gccgactgcg ccgccccaag ctcgcgcacg cgttcttcaa tcaccccgtc gtcggggttg
     4201 tcagcgtaga tcgcctcgtc gagcgcgcgg cgtgcccggc ggaggcgcat gccgagcagt
     4261 cggccttgcg gctcgatctc acgccggatc gcgttgatct gtgcgcgctg ttcgggcgtc
     4321 aggttcagcg cgcgcagcag cccgcctgcg ccgacgccgc gccgcgcttc cgacgcggtt
     4381 tcgggttcgt cgccgtcctg cgcgcgcacc gcgacgccgc tgccagccag tagcagcaac
     4441 gcgagcaacg cgtgcgcgag cacgaaaaac ctgcgcgaac tgaaattgtt cctgctctct
     4501 ctcatacctg acttaaacgt cgctttacgt ctctacacag cttgccgccg ggcgttttga
     4561 gcctagtttg cctcgatcaa aagatcgtag aggcgcggca cgtcatcttc gtcccggtcg
     4621 ctcctgagtc gcggctgccg cctgttcgaa ggggaaagcc gccgcgcacg gcgcgaattg
     4681 cttgcagccg gggcttccat ggatgatgcc gggacgggtt ccagcgtctc gaccgcagcg
     4741 atgagatttg ctgcgcggct gtcgtccaac tgtgcgcgcg tgtcttcgag ttcgcgcaac
     4801 gccgcgtcgc gctcggcgac gagactgtcc aactcggctt gcgtatagac tttcgcggcg
     4861 ggcgcggcaa cctgcgtcgt cgcctgcgcc gcgccactcc gcaggcgcgt gcggaaggtg
     4921 atcccgccgc cttcctcccg acgaacttcc gcgttgacga ttgcgagcgc ggcgagcgcg
     4981 cacacgacga gcgcggctgc tgccatcccg gcgcgcatcc acatgggcga gagcgtgaag
     5041 aactcacgca gggcggcgag cgcagaagaa gccccgcgcg gcgcaggaga agcggcgcgc
     5101 acactttcac cacgcgctcc ctgtgttgcc gtcgcgaccg taccggcgaa cgacggggca
     5161 gtgggtgcgg cgtggagaat ctctgcgcgc cagtcgccga tagcctcacg cactccggcg
     5221 aaagcgatga gttcgtcgcg gcacgcgtcg cattgcggca gatgcgcgtc gaaactctcg
     5281 cgctcgtccc gcgtcgcctc cccgtaaaaa taggcgacga gttctccggc gcgctcgcag
     5341 cagttggtgt tcgttgtctc gttcataact ttggtcttcc tcttttcgcg tcacacgtgc
     5401 gccgcttgcg cgccgtgctt ttccagacgc aggcgcagtt gtttgagcgc ggtgtagaga
     5461 cggctcttga ccgtgctcaa aggcacgtcg agcgtcgtgg cgatctcctg aaaagtcagt
     5521 tcctcaaact ctttcatcac gatcacttcc cgcagttccg gcgggagaga catcaccgca
     5581 cgacgcaccg ccgccgtccg ttcgcgccct tcgacctgcc gcgcgggcga ggcgtgcgct
     5641 ggcgcggcaa acttttcgcc gcccaactcc gtctccgttt cgagcgaatt ttcggcgcgc
     5701 gtgcgggcgc ggcgttggcg cgtgatgcac tggttgacgg cgatccggtg cagccatgaa
     5761 gagactttcg cttcgccgcg aaagccgcgc aggttgcgaa aggccgcgat gaacgtctcc
     5821 tgcgtcgcat cgtgcgcctc ctcttcgcgc cccaaaatcc cgtaggcgag cgcgtagata
     5881 cgacgctccc agcgttgcac gatctcgccg aacgcatcca catcgcccgc cagcgcacgc
     5941 tccactatct gctcgtcgct cgtttcagcc gtcgccattt ctcaacatct tccgccgggg
     6001 cttccggcgt ctcgcgccac gctcccggag tgcacgaagc ctctgcactc aactctttag
     6061 acgcgacaga cgcgccgttg gtcgaaaata ttgcaattaa ggacttatcg cgtgagcaaa
     6121 gttagcagat tatttgcctt gtcacgtaag caaagtcagc agattatttg cacgtaaaca
     6181 ttgacaccgc cagctcccag ttcgtactat gaaattggcc ttgtgcgtgc ttgtcaggcc
     6241 gcctcaaagt tcgattcttg cccgctccgg taaattttcc tctcttcaaa ctgcatcaca
     6301 cgaattccgt ctctctaagt atcattcatg cttgaacgtt tccggcgggt gcgcctgctc
     6361 tacattgtgc tcgggacttt gctcttcgtc ggcctcgtcc cgctcggcct cgcgggctgg
     6421 atgctctcga atcgcagcgc gaatgaactg cgctccatag aaggccgcta tcaggcgcaa
     6481 ctcgtgcagg acaaggcgcg gcagatcgaa ctctacggcc aacgctaccg cgatgtggta
     6541 acggggctgg cgcgcgcctt cgaactcgcg ggcggcgtcc gcgtgatcgg cgaagagggc
     6601 agcgatgcgc gtttgcagaa gtctgtggtg gacgatccga atctgctcgc gctcgccatc
     6661 ctgcccgtcg gcagcctccc gcacgtcgcc tacaagccgg acgtgatcgg gcgcgaagaa
     6721 ttgaacgagc gcgtgggtga agtgctcgcg cgcatgagcg ggcgcggcct cgtcgtgacg
     6781 cgcccgcagt tgatccgttc gagtcaggag atgagcctga cgattgccgc gcccgtgatg
     6841 ggcggcgcaa acggcgaaga ggtggtggcc gccgtggccg ccgtcatctc tttcaacgaa
     6901 gtcttcgcgg cagtccaaca gccgacttcc aagagcgaac gcgaactgct cgaacagggc
     6961 ttgcccgtca tcttcgtcgt cgatccgtcg gggcgcgcgg tggcgcaccc ggatcagcgc
     7021 gtcgcttacg cagagcgttc gatgagcgat cttctcatcg tgcaaaactg gatggacacg
     7081 ggcacgcagg tggaatcact gctcgaaccc ttcacggcgg aacgcgacgg gcgacagatc
     7141 gccatgctcg gcgcatatgc caccgccaaa ctcgacacgg gtgcgcgcct cggagtcatc
     7201 gccattcagg acgagcgcgc agccctgcgc tccgtcgcgg acatgcgcaa gcagacgttt
     7261 atgatcagct tgctcgccgc caccttcgcg ctgctcatcg ggtttctctt cgccaaacaa
     7321 ctgacgcagc ccgtgcgcga tctggccgac gccgccgggc gcatcgccaa tggcaatttc
     7381 gcgcagcgca tacaggtgcg cagccgcacc gaactcggcg aactcggcac ttcgttcaac
     7441 ttgatgaccg atcaactcga acagtatatc gaagacctgc gcagcgcggc ggaagagaat
     7501 cgtcaactgt tcgtcggcac ggtcaaggct ctggccgccg ccatcgacgg caaagaccgc
     7561 tacacgcgcg gccactccga acgcgtcgcg cgtttctcca tcgccatcgc cgagaatttg
     7621 ggtttgccag agggcgagat cgaagccctg cgcatcagcg cgctgctgca tgatgtgggg
     7681 aagatcggaa ttgacgataa ggtcttgaag aagcctgcga aactcacgga cgaagagttc
     7741 gagatcatga agacgcaccc gcaaaaaggg ttcaagatca tgtcgaacat tccggcgatg
     7801 cgcgagtttc tgccgggcat gtacatgcac cacgagatga tcaacggcga aggttatccg
     7861 caaggtttga agggcgacga gattccgatg caggcgcgca tcgtctccgt cgcagacacc
     7921 ttcgacgcga tgacgaccga ccgcccctac cagaaagcga tggaactcga agacgcgctc
     7981 gcgcgcatca aaactttcgt cggctcgcgc tacgacgaac gcgtggtgga cgcgctcgtc
     8041 gaagcctgtg agtccgggcg catcaacgcg gtcggcacga cgaggctgcg cccgcgtcgc
     8101 ctgccggaac gcgtggcagg gaacgtcccg cccgcagtcg aaccgcccgt cgagcagacg
     8161 caaactctgt aaacgctccg cagggcgcac acgccgccgc gcgtgcccga ctcttcattc
     8221 gtgcgagagt catttgtgcg agagtcattc gtacgagaga agttgcgcgt gctcgcctgt
     8281 cgagagcgcg agttgccagc gtccctcccg tttcatgtac acgtcaacta cgccgaaccg
     8341 gccattgtag ctgcccgcac gcgaacgcgc tttgagcgtg gcgaggccgg tgacgagggc
     8401 tgcatcgccc gccagacgca cgcgtatatc ttcgatctcg acggactcga aggcgaaggt
     8461 gccggaagcg aggtcttcga gccagaggtc tttcgtcacg gatgccccgc gtggatcggt
     8521 gaagacgaaa tcgtccgcga ggatgcgcgc gagcgacgcc gtatcgttgc gccggaaggc
     8581 ttcgatcaag tctagttcga gctgtctcac ctgtcgtttg tcgtcgttgt cgagaactgg
     8641 catatacact attcccccga agctaagtat aaacggttta catgccgcgc tcgcgcgtgt
     8701 gcttcatttg accggaatga tggcacagta atattcttcg tcaagtccag ttgaacacca
     8761 ctgcgcctaa tgaggaaaac atagatgctg cgtaaactgt cggtcttatc gatctgtctc
     8821 tggctggttc tgcccgcgct cgcgcaacag ccgacgccct cgccgagtcc ggacaagcgg
     8881 acggagcagt tggaagatgc tttgaggaaa gccgaggccg agaagcgtgc cgacaagtgg
     8941 gacgtggaag gcgaccatgg accaacgacg aaagttgagt tcgacacgga cgaaggcacc
     9001 tggatgagtt gcgacgtgtc gccggacggc acgcgcatcg tcttcgatct cctcggcgac
     9061 atctatcaca tgccctctgg cggaggccgc gcagagttgc tttcgggcgg cgtctcctgg
     9121 gagtcgcagc cgcgctacag ccccgacggc aagagcatcg cgttcacctc cgaccgcgac
     9181 ggcggcgaca acatctggct catggatgcg gacggcaaga accgtcgcca actgacgaaa
     9241 gagacactgc gcctcgtcaa cagtcccgct tggacgcccg acagtcaata cgtcctcgtg
     9301 cgcaagcact tcgtagatca acgctcgctc ggcgcgggcg aaatctggat gtaccacacg
     9361 ggcggcggcg ggacgggcgt gcagatgacg gagaagtcga gttggacggc caacgtcggc
     9421 gagcctgtca tcgatccgca gaagcgtttc atctacttcg tcaacagcgg cgacttcgac
     9481 tacaacaaaa acgtctacga gagcatttac tggatcgaac gctacgacac ggagcgcggg
     9541 cggcgcggcg tcttcgtgcg cggcgcgggc ggctcggtgc gcccgcaaat ctcgcccgac
     9601 gggaaactcc tcgccttcat ccgccgcgac cgcctcaaca cggtgctcta cctgcgcgag
     9661 atcgagagcg ggcgcgagtg gccgatctac gacggcttga cgcgcgacca gcaggagacg
     9721 tggtcggtct tcggcacgta ccccggttac ggttggacgc ccgacggcaa agccatcgtc
     9781 atcacggcga agggcaagtt cgtgcgcgtt gacgcacaga cgaaagcggc cacgccgatc
     9841 cctttcaccg cgcgcgtctc gcaacgcgtg gccgacgccg tgcgcttccc gcaaaaagtt
     9901 ttcgcggagc gcgacacgac gcgcctgcat cgttggccga aacgccacga agaccgcatc
     9961 atctacagcg cgctcggcaa gatttacgtc aagcagggca cagccgcgcc gcgccgcctg
    10021 ttgaactcca actacctcga atactcgccc aacttcagcc gcgacgggcg gcagatcact
    10081 ttcgtcacat ggtcggacgc ggagaagggc gcggtctgga tcgccgacgc cgacgg
//
LOCUS       DASWNS010000608         5523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_100769, whole genome shotgun sequence.
ACCESSION   DASWNS010000608 DASWNS010000000
VERSION     DASWNS010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100769"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..908)
                     /gene="ispH"
                     /locus_tag="VGB76_20835"
     CDS             complement(30..908)
                     /gene="ispH"
                     /locus_tag="VGB76_20835"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0101383.1"
                     /translation="MQVLLAEEYGFCFGVERAVEMVEGALSQGATVRTLGPLIHNTQE
                     IQRLESHGVATIETPADVEGEITAVIRAHGVTPQVQRELEARASHVVDATCPFVTKVQ
                     KLAERAAAEGRDVVVVGNPDHPEMIGVVGYAPANSHVVRDASEVANLPALHAPLVVSQ
                     TTIKLSTFLEAAEAVRGKADSEPQIVNTICSATRDRQDAARALATHVEAFYVIGGKHS
                     SNSLKLLSVCKEGCDRSFLIETPEEINAGDLRGVERVGVTAGASTPNWLIEQVVARLR
                     EIGEANREQAGAIAVN"
     gene            complement(1028..1315)
                     /locus_tag="VGB76_20840"
     CDS             complement(1028..1315)
                     /locus_tag="VGB76_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101384.1"
                     /translation="MNEANESRYHTGDTLCDALRFLSDASYAILPRDVAYRIAEFEKN
                     VLGGVRWIAEKKIGWIDEALRGGDRLREEWQRRCDRPPEAQTTTPPPEGMG"
     gene            complement(1404..1901)
                     /locus_tag="VGB76_20845"
     CDS             complement(1404..1901)
                     /locus_tag="VGB76_20845"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0101385.1"
                     /translation="MRRLVLCFTLLLCFVPSHASSSRAAQGFKFKVEDEINTLLRQFT
                     EAQKNYDAGKLDEILAPDYVEISPAGEVDPRAKVLGFYAPEKKSQRAGELVSYGLDEI
                     NTRIYGDTAIVVARLPFTMKTPDGQTASRALRCTFVCRKTRGKWRIASAQYTGIRPPQ
                     PPAKS"
     gene            complement(2015..2779)
                     /locus_tag="VGB76_20850"
     CDS             complement(2015..2779)
                     /locus_tag="VGB76_20850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0101386.1"
                     /translation="MNEGFYRALMVYGLASLLLASAACGLRETRAGKEGEDHMRAEQK
                     VAQNVAATAAATTPVAYVAFGDSTGVGVGARGGGYVARLFERIERVRGGSSVKNFCVS
                     GAETADVLRGQLTRLDGARPTLITLGIGINDVSHGVAPEQFARNYEEIITRLKSRTDA
                     PIVVTNIPDISTAPRVPVFLHDQVQARIRVFNERIAEIAGRHELFLVDAYDMSREVIP
                     AHPEFFSADGFHPSDEGYEYWAKMMWPTVKEAIGEE"
     gene            complement(2823..>5523)
                     /locus_tag="VGB76_20855"
     CDS             complement(2823..>5523)
                     /locus_tag="VGB76_20855"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0101387.1"
                     /translation="RAARSSARSRPVRRLLEGIGTPVRGEFKPDPFQLEAFAALEHED
                     VLVTAPTGSGKTWIAREEIRRLLAAGKRAWYTSPLKALTNSKYQEFSEAFGADKVGIL
                     TGDRKERADAPLIVGTTEVYRNQLFDALREGRELRTDLVIMDEAHYLADEDRGHVWEE
                     AIILTPPRVRLLLLSATVGNAEEFAAWVAEVRGVRCGVVTRPGARPVPLRAAFLYPDG
                     QLAPLFDEAGHFNPEIANFLQRSSAPQRHMSNRWGERREPQRGARMQMPEMPPASLLS
                     ALGSYDLLPSIVFLPTRRRCDEAAAEAALGRRDAQVERREARRTLMRDFTAEHAEIRK
                     HRHWNAVLNGGVASHHAGHLPAWKLLIEKLMSAGLLDAIFATATVAAGVDFPARTVVL
                     SNIDVRRGQGWRTLTASEFQQMTGRAGRRGRDRVGFIVAAPGTHQDPQKMAALLGALP
                     DPLESQFRATYTTLLNLLDAYGNFAQVREIAARSFAHRETVERVARLEQERAEAERRI
                     EQKLRDAGCEFSPDAARGLERLASARDKLLSSAPQTRTEVFLHWLVEAVVPGRVVGIG
                     RSSRRLVFVTERRDGNFVGVRENGHRASLDLTRVGRVYEEVYPLTEEARDAAFADVRE
                     GRATLMQEPRLRDARTGTDEAVDLINDLMEALTSSLVAASTAGETFDATVQEEARRRC
                     EEALWSALKDAETAERAERRIEALRAEVWQPFERRARVLEHFGYLDFDAERTTERGRW
                     LADLRLDRPLLVGEAIERNLFASLDAARAAGLMAALAADAERDYGELQLDDALVSTLA
                     EFERIAYEVASAEWRQGLDPAPEMNFSAAAAVMRWAKGMKWDELVRQTRAEEGDLFRL
                     LSRTGESLMQIGNLRESHAAAARVAYEAAESILREPVRSEELL"
BASE COUNT          938 a         1924 c         1662 g          999 t
ORIGIN      
        1 acttgatcca acgcggcaga gatcaataat caattgacgg cgattgctcc ggcctgctcc
       61 ctgtttgctt cgccgatctc gcgcagacgt gccacgacct gttcgatcag ccagttcggc
      121 gtcgaagctc cggcggtgac gcccacgcgc tccaccccgc gcaaatctcc ggcgttaatc
      181 tcttccggcg tctcgatcag aaagctccga tcacaacctt ctttgcagac cgagagcagc
      241 ttcaagctgt tcgaagaatg tttgccgccg atgacgtaga aggcttcgac gtgcgtggcg
      301 agcgcgcgcg cggcgtcctg cctatcgcgc gtagccgagc agatggtgtt gacgatctgc
      361 ggttcggagt cggccttgcc gcgcacggct tcggcggctt cgagaaaagt gctgagcttg
      421 atcgtcgtct gcgaaacgac gagcggcgca tggagcgcgg gcaggttcgc cacctcgctc
      481 gcgtcgcgta cgacgtgcga gttcgcgggc gcatagccga cgacgccgat catctccggg
      541 tggtctggat tgccgaccac gaccacatca cgcccctctg cggcggcgcg ctcggccagc
      601 ttctgcactt tggtcacaaa cgggcacgtc gcatcaacta cgtgcgatgc gcgcgcttcg
      661 agttcgcgct gcacctgcgg cgtaacgccg tgcgcgcgaa tgacggcagt gatctcgccc
      721 tccacgtcgg cgggcgtctc aatcgtcgcc acgccgtgcg attcgaggcg ttgaatctcc
      781 tgcgtgttgt gaataagcgg ccccagagtg cgcacggtcg cgccctgcga gagtgcgccc
      841 tcaaccatct cgacggcgcg ctccacccca aaacagaacc cgtactcttc ggccaacaga
      901 acctgcatgc gcgttccctt ccgactcttc gctgtgcgat ctttgagttt agactcgcgc
      961 tccgggagca acagtgcgct tcactgtcca gtcgtcctgc tgcgccaatc taaacccggt
     1021 acattctcta ccccatgccc tccggtggcg gcgtcgtggt ctgcgcttcg ggcggacgat
     1081 cacaacgtcg ctgccactct tcgcgcaggc ggtcgccgcc gcgcagggct tcgtcaatcc
     1141 agcctatctt cttctccgcg atccagcgca cgccgccgag gacgttcttt tcaaactccg
     1201 cgatacgata ggccacgtcg cgcggcagga tggcgtaaga ggcatcgctt aagaaacgca
     1261 gcgcgtcgca gagcgtgtcg cccgtgtggt agcgtgattc gttggcttca ttcataaata
     1321 gagatcactc cttcaccgtg gctatacgac cacacggacg cattcgttcc acccccgaga
     1381 tactagcaca gcgcaaacgt ttctcaggac ttcgcaggcg gttgcggcgg cctgatgccc
     1441 gtgtactgcg cggaggcgat gcgccacttg ccacgcgtct tgcggcagac gaaagtgcag
     1501 cgcagggcgc ggctcgcggt ctgcccgtcg ggagtcttca tggtgaacgg aagccgcgcg
     1561 acgacgatgg ccgtgtcgcc atagatgcgc gtgttgatct cgtccaaccc gtatgacacc
     1621 aactcgcccg cgcgctggct cttcttttcg ggcgcgtaaa aaccgagcac cttcgcgcgc
     1681 gggtcaactt ccccggcggg cgaaatctct acataatcgg gagcgagaat ctcatccaac
     1741 ttgcccgcgt cgtaattctt ctgcgcctcc gtaaactgcc gcagcagcgt gttgatctca
     1801 tcttcgactt taaacttaaa cccctgcgca gcccgcgaag aggacgcgtg cgacggcacg
     1861 aaacataaca gcaaggtgaa gcacagcacc aaacgtctca taagttctcc tcttccgttt
     1921 aagcagcatc agaaaacaaa gctcacgact tcaccgggtt tacgtcacgc aagtgacgca
     1981 agtttcgaca cgaccaaact caagcgcacg ctcgctattc ttcgccgatg gcttccttca
     2041 ctgtcggcca catcatcttc gcccagtatt cgtagccctc gtcggaagga tggaagccgt
     2101 ccgcagagaa gaactccgga tgcgcgggga tcacttcgcg gctcatgtcg taggcatcga
     2161 cgaggaacaa ctcgtggcgt ccggcgatct ccgcgatgcg ctcgttgaag acgcggatgc
     2221 gcgcctgcac ctgatcatgg aggaagacgg gcacgcgcgg cgcggtcgaa atgtccggga
     2281 tgttggtgac gacgataggc gcgtccgtcc tgcttttcag acgcgtgatg atctcttcgt
     2341 agttgcgcgc gaactgttcg ggtgccacgc cgtggctcac gtcgttgatg ccgatgccga
     2401 gcgtgataag cgtcgggcgc gcgccgtcga gccgcgtgag ttggccgcgc aacacgtctg
     2461 ccgtctccgc gccgctcacg caaaagtttt tcacgctcga ccccccgcgc acccgttcga
     2521 tgcgctcgaa gagacgcgcc acgtagccgc cgcctcgcgc tcccacaccc acgcccgtcg
     2581 aatctccgaa cgcgacataa gcgacgggcg ttgttgccgc cgccgtcgcc gctacgttct
     2641 gcgccacctt ctgttccgcc ctcatgtgat cctccccctc cttgcccgcg cgtgtctctc
     2701 tcaagccgca cgccgcgctc gccaacaaca gagacgccag cccgtaaacc atcaacgcgc
     2761 gataaaaccc ctcgttcata aaacctctct tgcccgtcaa actcatctct tccctgccaa
     2821 tttcacaaca actcttccga gcgcaccggc tcgcgcagga tgctctccgc cgcttcatag
     2881 gcgacgcgcg ccgctgccgc gtgcgactcg cgcaagtttc cgatctgcat gagcgactcg
     2941 cccgtgcgcg agagcaggcg gaagaggtcg ccttcttcgg cgcgcgtctg gcgcacgagt
     3001 tcgtcccact tcatgccctt cgcccagcgc atgaccgccg ccgccgccga aaaattcatc
     3061 tccggcgcgg ggtctagccc ctgccgccat tccgcgctcg ccacctcgta agcgatccgt
     3121 tcaaactccg cgagcgtcga gacgagcgca tcgtccaact gcaactcgcc gtagtcgcgt
     3181 tcggcgtccg ccgcgagcgc ggccatcagc ccggcggcac gcgccgcatc gagcgaggcg
     3241 aagaggtttc gttcaatcgc ttcgcccacg agcagagggc ggtcgagccg caggtctgcg
     3301 agccaacgcc cgcgctccgt cgtgcgttcg gcgtcgaaat cgaggtagcc gaaatgttcg
     3361 agcacgcgcg cacgccgctc gaacggctgc cacacttccg cgcgcaacgc ttcgatgcgg
     3421 cgttccgcgc gttcggctgt ctccgcatcc ttcaaggcag accacagagc ctcttcgcaa
     3481 cgtcgccgcg cctcctcctg aaccgtcgcg tcaaacgtct cgcccgccgt cgaagccgcg
     3541 acgagggaag aggtcaacgc ttccatcaga tcgttgatca gatcgacggc ctcgtccgtg
     3601 cccgtgcgcg cgtcgcgcag gcgcggctcc tgcatgagcg tcgcgcgccc ctcgcgtacg
     3661 tccgcgaaag ccgcatcccg cgcctcctcc gtgagcgggt agacctcctc gtagacgcgc
     3721 ccgacgcgcg tgagatcgag cgacgcgcga tgcccgttct cgcgcacgcc gacgaagttt
     3781 ccgtcgcgcc gctccgtgac gaagaccaga cgccgactgc tgcgcccgat gccgacgacg
     3841 cgccccggca cgaccgcttc cacgagccag tgcagaaaaa cttccgtgcg cgtctgcggc
     3901 gcgctactca gtaacttgtc gcgcgcgctc gccagacgct caagcccgcg cgccgcgtcc
     3961 ggcgaaaact cgcagcccgc gtcccttaac ttttgctcga tgcgccgctc ggcctccgcg
     4021 cgctcctgct ccagacgcgc gacgcgttcg accgtctccc tgtgcgcgaa actgcgcgcc
     4081 gcgatctcgc gcacctgcgc gaagttgccg taagcgtcga gcagattaag gagcgtcgtg
     4141 taggtggcgc gaaactggct ctccagcggg tcgggcaatg cgccgaggag cgcggccatc
     4201 ttctgcgggt cttgatgcgt gcccggcgcg gcgacgatga agcccacgcg gtcgcgcccg
     4261 cgccgcccgg cgcgccccgt catctgttga aactcggaag cggtgagcgt gcgccagccc
     4321 tgcccgcgcc gcacgtctat attcgagagg acgaccgtgc gcgccggaaa atcgacgccc
     4381 gccgcgaccg tcgccgtcgc aaagatcgcg tcgagcagtc ccgcgctcat caacttctcg
     4441 atcaagagtt tccaggcggg cagatgcccc gcgtgatgcg aggcgacgcc gccgtttaaa
     4501 accgcgttcc agtggcgatg cttgcgaatc tccgcgtgct cggcggtgaa gtcgcgcatg
     4561 agcgtgcgcc gcgcctcgcg tctctcgacc tgtgcgtcgc gtctgccgag cgcggcctcg
     4621 gcggcggcct cgtcgcagcg gcggcgggtc ggcaaaaaga cgattgacgg caggaggtcg
     4681 taggagccga gcgcggacag aagcgaggcg ggcggcatct ccggcatctg catccgcgcg
     4741 ccgcgttgag gctctcgacg ctcgccccaa cgattcgaca tgtgacgctg cggcgcgctt
     4801 gagcgttgga ggaaattcgc gatctccggg ttgaagtgtc ccgcttcgtc aaagagcggc
     4861 gcgagttgcc cgtcaggata aaggaaggct gcgcgcagcg ggacgggacg cgcgcccgga
     4921 cgtgtgacga cgccgcaacg cacgccccgc acctccgcca cccatgccgc gaactcttcc
     4981 gcgttcccga ccgtcgccga cagcagcagc aggcgcacgc gcggcggcgt caaaatgatc
     5041 gcctcctccc agacgtgccc gcgatcttcg tccgcgaggt agtgcgcctc gtccatgatc
     5101 acgaggtctg tgcggagttc gcgcccttcg cgcagggcgt cgaagagttg gttgcgataa
     5161 acttcggtcg tgccgacgat gagcggcgcg tcggcgcgtt ccttgcggtc gcccgtcagg
     5221 atgcccacct tgtctgcgcc gaacgcttcc gaaaattcct gatactttga attggtgaga
     5281 gccttgagcg ggctggtgta ccaggcgcgc ttgcccgccg cgagcagccg cctgatctct
     5341 tcgcgcgcga tccacgtctt gccgctgccc gtcggcgcgg tgacgagcac gtcttcgtgt
     5401 tcgagcgcgg cgaaggcttc gagttgaaac ggatcgggct tgaactcgcc gcgaacgggc
     5461 gtgccgatgc cttccagcag acgccttacg gggcgcgaac gcgcagacga gcgcgccgcc
     5521 cgt
//
LOCUS       DASWNS010000609         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_283163, whole genome shotgun sequence.
ACCESSION   DASWNS010000609 DASWNS010000000
VERSION     DASWNS010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283163"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..>3657
                     /locus_tag="VGB76_20860"
     CDS             11..>3657
                     /locus_tag="VGB76_20860"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY0101388.1"
                     /translation="MKNFTRWVLLASIAVAVSAVVFNFGPVASSQSSRKGERSKVNPT
                     SPGRTGLENFDIRVRRAGEAEAAATRKGGGLTAEAQKQVVATQRQREAAAVVQQSMRA
                     AEQSLAARLPNLKVNYNAALRVPEIVKVEGAGVLASAAANSKEEALRSFVSENAALYG
                     LTRGQAAQLTKFSDYTNPAGNLSFVELEQQINGIPVFQGFVRGVLTPQGQLMSTVSLL
                     AAGMRPSDLDTTPGIDAAEAVAAGIKSINLKVNAASLSVLDAAADGKSHVVSRGPLDE
                     ETKTSLMYFPVVPGHAVLAYSMTLWGPENSYYVFVDANTGQLLWRKNITQDQQTAYTY
                     NIYDNDSPTPSAPVRSSLISPVNTTASEPQPPGISRTDVTRVSESPFDNLGWIPDGAG
                     PNAPTTGNNVDAGIDRVAPNGIDPGGRATATNFVFRFNYRPDGSTDPVGSNNPLDPEF
                     QKGAVTNLFFWSNRYHDSLYHLGFTEAARNFQTNNFGRGGLGNDAVRAEAQDSSGTNN
                     ANFNTPADGQPPRMQMFLWTGTPVRDGDLDQEVMYHELTHGTSNRLHANGSGLGSDTA
                     QGMGEGWSDFYALSLLAMHTPDGDDGKRLYAMGAYDTRNYYRAIRRFPYAVKSAVAAN
                     GRSHNPLTLADIDPAQLDVSDAAFPVAPGQATQVHNQGEVWCSALIEVRAKLIEKLGF
                     ANGSQRVLQIVTDGMKLDPVNPTMLDARDSIIAADCAGFNGGDELDIWEGFRIRGMGF
                     RAATSGIHVTENFESPNLKLLTITATEVPGSSNGDGVIDPGETARISVPLQNILCSVT
                     VPSATATLSPGGDTATYPAFAPGAVNTETLDYTVPANTACGSSVPLTITVNSTLGPIT
                     YNYNLNIGRPSPLAPYENFDNVTPPALPLGWTTQRTGAGIPWQTTPTNPDTPPNSVFT
                     TNPTNAGTSDLLSPTFPVNTALAQVSFRNSFNLEDNFDGMQLHIKIGNGPFREIIAAG
                     GSFVSGGYNGEINDGAAWTGLSGGTSANPTYITSTVNLPPSANNQLVQLKWTVIGDAN
                     TIAPGQSGARIDTVQLSTTAQACNPFGVFTVSISGHLRDSVGNPVAGIQVTLSGTTNA
                     TATTNADGFYSFPNLVSGGDYTVAPTTPGFDYLPQSRTYNDLTTNVTDADFTVAFAAS
                     ISGRVTSENGAVGIDGITITLTGTESRTTVTSGGGFYSFQNLTRGGNYTVTPSGGNNT
                     FTPPSRTYNDL"
BASE COUNT          739 a         1289 c         1026 g          603 t
ORIGIN      
        1 gatttcgcat atgaagaatt ttacacggtg ggtactgcta gccagtatcg ccgtcgccgt
       61 gagcgccgtg gtgttcaact tcggccccgt cgcctcaagc caaagctcgc gcaaaggtga
      121 gagatcgaag gttaacccaa cttctcccgg tcgcaccggc ctggaaaact tcgacatccg
      181 tgtcagacgg gcgggcgaag cggaagccgc cgcgacacgt aagggcggag ggctgaccgc
      241 ggaggcgcag aaacaggtcg tcgccacgca acgccaacgc gaagccgccg ccgtcgtgca
      301 gcagtcaatg cgcgctgcgg agcagtctct ggctgcgcgt ctgcccaacc tgaaagttaa
      361 ttacaacgcc gccttgcgcg ttcccgagat cgtgaaggtc gaaggcgcgg gcgtgcttgc
      421 gtctgccgct gcgaactcga aggaagaagc cctgcgcagc ttcgtctcgg agaacgccgc
      481 gctctacggt ttgacccgtg ggcaggcagc gcaactcacc aaattttccg attatacgaa
      541 cccggcggga aacctctcgt tcgtggaact cgaacaacag attaacggca tccccgtgtt
      601 tcaagggttc gtgcgcggcg tcctgacgcc gcaaggccaa ctcatgagca cggtcagcct
      661 gctcgccgcc ggcatgcgcc cctccgacct cgataccact cccggtattg acgccgcaga
      721 ggccgtcgcc gccggtatca agagcatcaa tctgaaggtc aacgccgcga gcctctccgt
      781 gctcgacgcg gctgccgacg gcaaatcgca cgtcgtttcg cgcggcccgc tcgatgaaga
      841 gacgaagacc agtttgatgt atttcccggt cgtgccgggt catgccgtgc tggcctactc
      901 catgaccttg tggggaccgg aaaactccta ctacgtcttt gtcgatgcga acacgggtca
      961 actcctgtgg cgcaagaaca tcacgcagga ccagcagacc gcctacacct acaacatcta
     1021 cgacaacgac agcccgacgc cctccgcgcc cgtccgctcg tcgctcatct cgcccgtgaa
     1081 caccaccgcg agcgagccgc agccgccggg catctcgcgc acggacgtta cgcgcgtctc
     1141 cgagtctcct ttcgacaacc tcggctggat tcccgacggc gcaggcccga acgccccgac
     1201 gaccggcaac aacgtggacg ccggtatcga ccgggtagcc ccgaacggca tcgaccccgg
     1261 cgggcgcgcg acggcgacga atttcgtctt ccgtttcaat tacagacccg acggctcgac
     1321 cgatcccgtc ggctcgaaca acccgctcga tcctgagttc cagaagggcg cggtcaccaa
     1381 cctcttcttc tggtcgaatc gctatcacga ttcgctctac catctcggct tcaccgaggc
     1441 ggcgcgcaac ttccagacga acaacttcgg gcgcggcggc ctcggcaacg acgctgtgag
     1501 ggcggaagcg caggattctt ccggcaccaa caacgccaac ttcaacaccc cggctgacgg
     1561 tcagcccccg cgcatgcaga tgttcttatg gacgggcacc ccggtgcgcg acggcgacct
     1621 cgaccaagag gtcatgtatc acgaactgac gcacggcacc tcgaatcgcc tgcacgccaa
     1681 cggcagcggt ctgggcagcg acacggcgca gggcatgggc gaaggctggt cggacttcta
     1741 cgccctttcg ctcttagcca tgcatacgcc cgacggcgac gacggcaaac gcctctacgc
     1801 gatgggcgcg tacgacacga ggaactatta tcgtgccatt cgccgcttcc cttacgcggt
     1861 gaaatcggcg gtggccgcca acggcagatc gcacaatcct ctgactctgg ccgacatcga
     1921 cccggcgcaa ctcgacgtga gcgacgccgc tttcccggtc gcgcccggtc aagccacaca
     1981 ggttcataat cagggcgaag tctggtgctc cgcgctcatc gaagtgcgcg cgaagctgat
     2041 cgaaaaactc ggctttgcca acggcagcca gcgcgtcttg cagatcgtca ccgacggtat
     2101 gaaactcgac cccgtcaacc cgacgatgct cgatgcgcgc gattcgatca tcgcggcaga
     2161 ctgcgcgggc ttcaacggcg gcgacgaact cgacatctgg gaaggcttcc gcattcgcgg
     2221 catgggcttc cgcgccgcca cctccggcat ccacgtcacg gaaaacttcg aaagcccgaa
     2281 cctcaaactg ctgaccatca ccgcgacgga agttccgggc agcagcaacg gcgacggcgt
     2341 catcgatccg ggcgaaaccg cgcggatcag cgttccgctc cagaacatcc tgtgctcggt
     2401 caccgtgccg agcgcgaccg cgaccctttc accgggcggc gatacggcca cctatccggc
     2461 gttcgctccc ggcgcggtca acaccgagac gctcgactac accgtcccgg ccaacaccgc
     2521 ctgcggcagc agcgtgcctt tgacgatcac cgtcaacagc accctcggcc ccatcaccta
     2581 caactacaac ctgaacatcg ggcgaccgtc tccgctcgct ccttacgaaa acttcgacaa
     2641 cgtgacgcct ccggcgttgc ccctcggctg gacgacgcaa cgtaccggcg cgggcatccc
     2701 gtggcagacc acgccgacca acccggacac tcctccgaac tcggtgttca ccacgaaccc
     2761 gaccaacgcg ggcacgtccg atctcttgtc gccgaccttc ccggtcaaca ccgcgctggc
     2821 tcaggtcagc ttccgcaact ccttcaacct cgaagacaac ttcgacggaa tgcagttgca
     2881 catcaagatc ggcaacgggc cgttcaggga gattatcgcg gcgggcggca gtttcgtcag
     2941 cggcggttac aacggcgaga tcaacgacgg ggcggcctgg accggactct ccggcggaac
     3001 ctctgccaac ccgacttaca tcacttccac cgtcaatctg ccgccttcgg ccaacaacca
     3061 actcgtgcag ttgaagtgga cggttatcgg cgacgcgaac accatcgcgc cgggacaatc
     3121 gggcgcacgc attgacacgg tgcaactctc gacgaccgcg caggcttgca atcctttcgg
     3181 tgtcttcacc gtttcgatca gcggccattt gcgtgacagc gtcggcaacc cggtggcggg
     3241 catccaggtc acgctgagcg gcacgacgaa cgccacggct acaaccaacg ccgacggctt
     3301 ctattcgttc ccgaacctcg tttcgggcgg cgactacacc gttgcgccga ccactccggg
     3361 ctttgattat ctgcctcaga gccggactta caacgacctg acaaccaacg tgaccgacgc
     3421 cgatttcacc gtcgccttcg cggcgagcat cagcggtcgc gtcacttcgg agaacggcgc
     3481 agtgggcatc gatggcatca ccatcacgct caccggcacg gaatcgagaa caaccgtcac
     3541 ctccggcggc ggcttctact cgttccaaaa tctgacgcgc ggcggcaact acaccgtcac
     3601 gccttccggc ggcaacaaca cgttcacgcc gccgagccgc acttacaatg acctgca
//
LOCUS       DASWNS010000610        14320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_515951, whole genome shotgun sequence.
ACCESSION   DASWNS010000610 DASWNS010000000
VERSION     DASWNS010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14320
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515951"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VGB76_20865"
     CDS             complement(<1..618)
                     /locus_tag="VGB76_20865"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0101389.1"
                     /translation="MTDELLKWRAEFPILDKTTYLISHSLGAMPRATFDNLQAYAEAW
                     ATRGVRAWGEGWWEMPLTIGDEVARIIGAPPASVVMHQNVSICQSLILSCFDDDARRN
                     KIVYSDLEFPSVMYAYEAHARHGRFRVERVPSDDGMTVSLERMLAAIDEETLIVPISH
                     VLFKSAFLQDARAITERAHEVGALVVLDTYQSAGTVPFSVTELDVD"
     gene            complement(896..3781)
                     /locus_tag="VGB76_20870"
     CDS             complement(896..3781)
                     /locus_tag="VGB76_20870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0101390.1"
                     /translation="MKTRTILVGIFFALVAAYTPASYSSWITLQHMLEERGWNAAPSA
                     PGGPELIVWVNASGPATDLQVGDEIIAINGQNVERSDYRMENTFRRLNRDEAYTLTIR
                     RDGELRDITLRRNHVSLLRRVFTSGAFSTLLSSAIFLLIGIAVFVVKPEDKQALLLSL
                     WLVLMASAFSLPQPFEGTAWWYRALIVFGQATKVFAPSFLLHLFLVFPEPSPILKRWP
                     RLERYLYLPTLLLSFLPITYMLILWAVDPEELIAFSLRYPWIGVISAILLPLTILLAL
                     LSLVLSYRRAGQVSKRKLRIVLVGSLVAFMPFVGWFLILAVVPRSREGWAPYVGWLIV
                     NTLMLAMPISFAYAIVRHQVIPVKLIIRRGLQYLLARNALRLLIALPVVGLCVSIISN
                     RERRLSEILLGSSLYFYLFIAAIALALVFRKRLGEWVDRKFFRESYNQEKILRELTDD
                     VKKLDSIPEMSKRVTERVTAALHPQQAFLFYREEGTRDLSLTYTSGGATLTGQELKIP
                     EEFRLLRFMEDHGSAQEFPFPQKNSLPQSEKTWLASLGTRLIVPLTGTDERLAGLLLL
                     GEKKSEVPYTASDRELLETLAGQIAIVYENVRLKNRVDRDRKIKQEVLSRVAGQDMNV
                     LKECPQCGACFDMNAQACDRDAAELTLSLPVERTIETRYRLERSIGKGGMGAVYEATD
                     LRLHRKVAVKILTGSMFGNSEALRRFEREAQASARLHHPNIITVHDYGLLSTEGAYLV
                     MELIAGETLGARIRRERQLAPAVAADLFEQILDGLKAAHASGVVHRDLKPENVLLSTY
                     ENGQTRAHLLDFGLAKLMQALPADSLSPTAAAPVTTPGAVMGTFGYMSPEQLTGGAVD
                     ERTDIFSIGVMVVEAVTGRRPFTGATYHELLTNILNAPFHLEGSSPAALALDAVLQRS
                     LAKDRAARYADAAAMQRELIPALRQCQPFVSPDEPTALDAETFILKN"
     gene            4077..5579
                     /locus_tag="VGB76_20875"
     CDS             4077..5579
                     /locus_tag="VGB76_20875"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101391.1"
                     /translation="MTWHPANPIRHSLPGARWPTRAEAARALLFQPVDIGSLTLEQRT
                     WVPAMVPWRATEDGFVTREVLEWYGRFAAGRPGAIVVEATGVRDIPSGPLLRIGHERF
                     LPGLRELVETVRRASDGHTRLFIQIIDFLSVKRRPEKSKYFARFLQLEERHARALAAA
                     TGDESWLRAGETETRAFLAAAPEELLGKVLDERELESLTRGYRERVTDMHLAHVEALP
                     RVLPEIFAAAARRAREAGFDGVELHYAHAYTMASFLSALNTRPDGYGGSRENRVRLPL
                     EVYRAVRERAGRDYTVGVRFLGDEVIAGGSRLEDAIYFGREFARAGFDFLSLSKGGKF
                     EDAAQPKVGHAVYPYTGESGYECMPTIRSDERGPFGRNVSLAAAVRRALLDEGSHTPV
                     VVAGGICTFEQAEEILRRGEADIIGAARQTLADPDWFLKTRLGKGDEIRRCTYTNYCE
                     GLDQAHKQVTCKLWDRVQLDEPNISLSHDRRRRLLAPDWKVEEDGEKGTG"
     gene            complement(5632..6768)
                     /locus_tag="VGB76_20880"
     CDS             complement(5632..6768)
                     /locus_tag="VGB76_20880"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0101392.1"
                     /translation="MKIVIIGGGPAGLYFALLMKKHEPSYEITVLERNAPDDTFGWGV
                     VFSDKTLSYLRDTDEETYREITRSFATWDNVDVVHRDERVTIRGNKFSGIARIKLLNI
                     LQARCRALGVELRFRAEVGNLEEFSGCDLIVGADGVNSTVRERYRDSFEPSLGVRHNK
                     YIWYGTHQLFHGLTLTFRESAAGVFAAHSYKFDPQTSTFIVECDAETWAGAKFAEMTE
                     AETRAYLEAVFARDLGGHALLSNNSKWINFLLVKNRHWQHENVVLLGDALHTAHFSIG
                     SGTKLALEDAIALYRCLAANSNVRGALAEFERVRKPVIEEYQQAAYESLVWFEHARDY
                     MHLAPLPFAYSLMTRSKKIDYENLKRRDPAFIEAYEAAIKSASD"
     gene            complement(6850..7926)
                     /locus_tag="VGB76_20885"
     CDS             complement(6850..7926)
                     /locus_tag="VGB76_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101393.1"
                     /translation="MDQEKDLPPSVKPESNESQQRPKSLWLRIVRWLDRLLWLGFLLW
                     FARLLWLGFLLWFTRLLWLDRLLWFDFLLWLGFLLLLLFPLKSLFLCIRFSEIWHGRY
                     PAYYHCDYPPSREDIRQHKLKTRRFSRARFFGPKEFENKWFSLDMFICIPKHLRYYES
                     WEERLREKHKRFLSFASPPETPQIVSGINVELLKEQYQMQIRLYEKYLDLLIRFNFLN
                     FAITGAILSFSLQSGEQNLVRWSFAFPFFMNLSFALLILLAFRSLKIINREIIYMSEF
                     LGFQPPGVRTLRHALAVSFFTLLLVALVLFPTAFNVCDLKMYKVNVNWILSNRPSPTA
                     PTYPCNTPEDDLAARQREQEANPR"
     gene            complement(8010..9197)
                     /locus_tag="VGB76_20890"
     CDS             complement(8010..9197)
                     /locus_tag="VGB76_20890"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0101394.1"
                     /translation="MDRFIEETPFFTPEHLRLAASVASFVWREIEPRAEGDEEETDQA
                     QSDQKFRELVSLLAQAELLRYAVARPHAPFDVRALCLIREELSYSSPLADLAFVMQGL
                     GTHAVSLAAPEHVRDFWLTRAATGRAVAAFALTEPEVGSDISAIQTTARRDGDSYVLD
                     GRKRFISNAGIADFYTVFARTGSRPDGRAEISAFVVSARMNGFTVRERTRLISPHPIG
                     EIEFNNLRVPAEDRVGEEGEGFRLAMLTLGTFRASVGAAACGMARRALDEAIAYARRR
                     TQFGQPLAAHQLIQEKLADMSAELDAARLLVYRSAYLRDTYGQEDTYAPTLSRAASTA
                     KLYATEAAQRIIDQALQIHGGAGLVHGHIVERLYRDIRALRIYEGTSEIQKLVIAREL
                     LKE"
     gene            complement(9278..10090)
                     /locus_tag="VGB76_20895"
     CDS             complement(9278..10090)
                     /locus_tag="VGB76_20895"
                     /inference="COORDINATES: protein motif:HMM:NF006107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEY0101395.1"
                     /translation="MLSPKSFLYEVRADGVAWITLNRPERLNALTFEVYRELTDTFAA
                     LADEADVRAVVITGAGRAFCSGGDVRDIIADLQSLDIQGHLAFTRLTCELIRNMRVLR
                     KPVIASLNGAAAGAGAVVALAADLRIAAMEARIAFLFVRVGLSGADMGAAYLLPRYVG
                     LAKATELLYTGDFISAAEAERIGLYNRVVPAAELEAETRALAGRLARGPAFALGMTKE
                     MLNREMHVDLDTALEWEAQAQSVCMQHPDFREAHEAFNAKREPVFKGVMSNE"
     gene            complement(10263..10679)
                     /locus_tag="VGB76_20900"
     CDS             complement(10263..10679)
                     /locus_tag="VGB76_20900"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0101396.1"
                     /translation="MFKLINPASLGRPRGYANGVLAPEGGALLFIAGQIAWDERQQIV
                     STDFVAQFERALMNVLAVVSEAGGAPEQIARLCIYVTDKEEYRSRQSEIGACWRKLLG
                     RHFPAMALVEVKALLEDEAKVEIEGIAVIRAVTADK"
     gene            complement(10777..11559)
                     /gene="fabG"
                     /locus_tag="VGB76_20905"
     CDS             complement(10777..11559)
                     /gene="fabG"
                     /locus_tag="VGB76_20905"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY0101397.1"
                     /translation="MELKGKIALITGGGRGIGRAIAFAFARAGASLALCSRTGSEIEA
                     VADEIRREFRVDVLNDVCDVSDPKDVANFFLATYNGFGRHADILVNNAGIAESAPITR
                     TDDAHWRRHLAINLDATFYCTRAALPAMLERGWGRVINIASIAGKTGAPYIAAYAASK
                     HGVVGLTRSVALEVAAKGVTVNAICPGYVDTDMTTRALEQIEAKTGRTTADALEAIKR
                     MSPQNRLVTPEEVAALALLLASEDGRGINGQAINVDGGSVVS"
     gene            complement(11622..12407)
                     /locus_tag="VGB76_20910"
     CDS             complement(11622..12407)
                     /locus_tag="VGB76_20910"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY0101398.1"
                     /translation="MEQFIKVQVRERILTITLDRAEKLNAFAGHMRRDLGETLERAGS
                     DDDVRVIVITGAGRAFCAGGDVAAMDEMMEREDADEFARMLGAGRRVVTAIRSMVKPV
                     LASINGTASGAGFNLALACDLRIAAESARFSQSFVKVGLHPDWGGTYFLPRIVPSNIA
                     CEMFFLGDAIDSARALTLGLVNRVVPDEQLAAETRRLAERLRDAPPESIAAAKHAVYM
                     SHHAELEEMLQYETEAQLRCFQTTDAREGVRAFLEKRAPRFSK"
     gene            complement(12521..13357)
                     /locus_tag="VGB76_20915"
     CDS             complement(12521..13357)
                     /locus_tag="VGB76_20915"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0101399.1"
                     /translation="MVEAEAKMQGTEEAVGTLVGYRAEDGVALLTLNDPPANTYSYEM
                     MGELDRAILRARMDESVQVIVLTGSGEKFFCAGANIGMLQSVTPTFKYYFCLHANETL
                     SRLEQTPKLVIAAINGHCVGGGLEVAMAADIRIARRGAGRMGLPEVSLGVLPGTGGTQ
                     RLVRMVGKSRAIELMTTGELFDFERGAELGLVNHIYEAESGAAFMQQVLAYAAKFTLP
                     DKAAHAVGRIKRAVQTGAEIPFESALALERELQQQLFQSEDAREGLDAYVGKRKPQFK
                     GR"
     gene            complement(13382..14089)
                     /locus_tag="VGB76_20920"
     CDS             complement(13382..14089)
                     /locus_tag="VGB76_20920"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0101400.1"
                     /translation="MASNLSTDFSRYDQKLEFILRTSARIFAEKGYHSTSMRDIARAT
                     EVSLAGLYYYCKSKEELLFLIQDNCFGRVHERLQERLRETTDAVAKLRLVIENHLSFF
                     AANMAEMKVLSHEADSLAGEMHAHVTGRKQQYTRLVRRILSEVQQAQQAREGHESGVQ
                     KLDLTVATYALFGMMNWIYNWYDPRGKLSVGDLVDNLTRLFLSGFLAGQAGHELQFTR
                     KTGAAAERLSVWRNTNT"
BASE COUNT         2853 a         4240 c         4482 g         2745 t
ORIGIN      
        1 gtctacgtcg agttcggtga cggagaaggg cactgtgccc gccgattgat atgtgtctaa
       61 gacgacgagc gcgccgacct cgtgtgcgcg ttcggtgatg gcgcgcgcgt cttgcagaaa
      121 cgcgctcttg aagaggacgt gcgagatggg aacgatgagc gtctcctcgt caatggcggc
      181 gagcatgcgt tcgagcgaga cggtcatgcc gtcgtccgac gggacgcgct cgacacgaaa
      241 gcgaccgtgg cgcgcgtgcg cttcgtaggc gtacatgacg gagggaaatt cgagatcgga
      301 atagacgatc ttattgcggc gcgcatcgtc gtcgaagcag gagaggatga gcgactggca
      361 gatggagacg ttctggtgca tgacgaccga ggcgggcggc gcaccgatga tgcgcgccac
      421 ttcgtcgccg atggtgagcg gcatctccca ccagccctcg ccccacgcgc gcacgccgcg
      481 cgtcgcccac gcctcggcgt aggcttggag gttgtcgaaa gtggcgcggg gcatcgcgcc
      541 gaggctgtgc gagatcagat aagtcgtctt gtcgagaatc ggaaattcag cgcgccattt
      601 gagtagctcg tcggtcaaac gtttggttcc tcctgcgtgt ggacaatgat atgcggcggg
      661 agcactgaac gtctagcgag cggggtggcc ggagtgagaa gctcagcttg tgcttgagtc
      721 gccggagagc gcatttcaac acgagaggtg cagagttcac gcagaggcgc agagttcacg
      781 catgggcgcg ctgaggcttg aataatcttt aactctctgt ggttctctgc gtggactctg
      841 cgcctctcgt tgcgcctctc gtgttgaata accgtttgcg gttattcagc cacggttaat
      901 tcttcaagat gaacgtctcg gcatcgaggg cggttggctc gtccggcgag acgaacggct
      961 gacattgacg cagcgcggga atcaactctc gttgcatggc ggcggcatcg gcataacgcg
     1021 cggcgcgatc tttggcgagc gatctttgca gcacggcgtc gagcgcgagc gcggcgggcg
     1081 aagagccttc gagatgaaac ggcgcgttga ggatgttggt cagcagttcg tgataggtcg
     1141 cccccgtgaa gggacggcga cccgtgacgg cttcgacgac catgacgccg atggagaaga
     1201 tgtctgtgcg ttcgtcaacc gcgccgcccg tgagttgttc gggcgacatg tagccgaagg
     1261 tgcccattac cgcgccgggc gtggtgacgg gcgcggcagc ggtcgggctg agcgaatcgg
     1321 cggggagcgc ctgcatgagt ttggcgaggc cgaagtcgag caggtgcgcg cgcgtctgcc
     1381 cgttttcgta ggttgagagc aggacgttct caggtttgag gtcgcggtgg acgacgcccg
     1441 acgcgtgcgc ggctttaagg ccatcgagaa tttgctcgaa gagatcagcc gcaacggcgg
     1501 gcgcgagttg ccgctcgcgc ctgatacgcg cgccgagcgt ttcgcccgcg atgagttcca
     1561 tgacgagata cgcgccttcg gtcgagagca aaccgtagtc gtggacggtg atgatgttcg
     1621 gatgatggag tcgtgcgctg gcctgcgcct cgcgctcaaa acgacgcaga gcctcgctgt
     1681 tgccgaacat gctgcccgtt aaaatcttga cggcgacttt gcgatgcagg cgcaggtcgg
     1741 tcgcttcata gaccgcgccc atgccgcctt tgccgatgga gcgttcgagc cggtagcgcg
     1801 tctcaatcgt gcgctcgacg ggcagcgaga gcgtcaactc cgccgcgtcc cggtcgcacg
     1861 cctgcgcgtt catgtcgaaa cacgcgccgc actgcgggca ctccttcagg acgttcatgt
     1921 cctgcccggc gacgcgcgag agcacctcct gtttgatctt gcgatcccgg tctacgcggt
     1981 tcttcaggcg cacgttctca tagacgatgg cgatctgccc ggcgagcgtt tcgagcagtt
     2041 cgcggtcgct cgccgtgtag ggcacttcgg atttcttctc gcctaagagg agcagccccg
     2101 cgagccgctc gtctgtgccg gtcagaggca cgatgaggcg cgtgcccaaa cttgcgagcc
     2161 acgttttttc gctctgcggc aggctgttct tttggggaaa cggaaattcc tgcgcgctcc
     2221 catgatcttc catgaagcgc agcaggcgaa actcttccgg tatcttcaac tcctgccccg
     2281 tcagggttgc gccgcccgac gtgtaggtga gcgagagatc gcgcgtgccc tcctcgcggt
     2341 agaagaggaa ggcctgttgg ggatgaagcg cggcggtcac gcgttcggtg acgcgcttgg
     2401 acatttcggg aatcgagtcg agcttcttca cgtcgtccgt caactcgcgc agtatcttct
     2461 cctgattgta cgactcgcgg aagaacttgc ggtcaaccca ctcgccgagc cgtttgcgga
     2521 agacgagtgc gagcgcgatg gccgcgatga acaggtaaaa gtagagcgaa ctgccgagca
     2581 ggatttccga caaccgccgc tcccggttag agatgatcga gacgcagagg ccgacgacgg
     2641 gcagcgcgat gagcagacgc agggcgtttc tggcaagcag gtattgcagc ccacgccgga
     2701 tgatcaattt gacggggatc acctgatggc gcacgatggc gtaggcgaac gagatcggca
     2761 tcgcaagcat cagtgtgttg acgattaacc aacccacata cggcgcccag ccctcgcgcg
     2821 agcgcggcac cacggcgagg atcaaaaacc acccgacgaa cggcatgaag gcgacgaggc
     2881 tgccgacgag cacgatgcgc aacttacgct tcgacacctg acccgcgcgc ctgtaactca
     2941 gcacgagcga cagcaatgcc agcagaatag tgaggggcaa gaggatcgcg ctgatcaccc
     3001 cgatccatgg gtagcgcagg ctgaaagcaa ttaactcctc ggggtctacc gcccagagga
     3061 tcagcatata agtgatgggg agaaagctca gcagcagcgt cggcaggtag agatatcgtt
     3121 ccagtcgcgg ccagcgtttg aggatgggcg acggttcggg gaagacgaga aacaggtgga
     3181 gcagaaagct cggcgcgaat accttggttg cctgcccgaa cacgatcagg gcgcgatacc
     3241 accacgccgt cccttcaaac ggctgcggga gcgaaaaggc ggaggccatc agcacgagcc
     3301 acagagagag cagcagagcc tgtttgtctt ccggcttgac cacgaagacg gcgatgccga
     3361 tgagcaggaa gatggcggaa ctgagcagcg tggagaatgc gccggacgtg aagacccgtc
     3421 tcaatagaga gacgtggttg cggcgcagag tgatgtcgcg cagttcgcca tcgcgccgga
     3481 tggtgagggt gtaggcttcg tcgcggttga ggcggcgaaa ggtgttctcc atgcggtagt
     3541 ccgagcgttc gacgttctgg ccgttgatgg cgatgatctc gtcgcccacc tgcaagtccg
     3601 tggcggggcc gctcgcattc acccagacga tcaactcggg gccgccgggc gcgctcggcg
     3661 cggcgttcca accgcgctcc tccaacatgt gttggagggt gatccatgaa ctgtagctcg
     3721 caggggtgta ggccgctacc agagcgaaga agattccgac gaggatggtg cgtgtcttca
     3781 tgtgcgtgcg gcctcaaccg ctgaagcgtt gctcactatc gaagactaac ggctggcttc
     3841 aagtcagcct gatgcgcaag aggaaacgac gcgcgggtgg taaaagttcg gttgcagaaa
     3901 agcggcgacg cgagtgcgcc cgtgccggcg gatgcatgtc gcgcggcgca tcttaacaaa
     3961 cttccgcgcg cgcgtcagtc ctttcgtcgc aggcgtgtgc tcgaaggagc cggacaaaac
     4021 cctgcctgtt ttcgtccgct cgcgcggaac tgctacactg cacgactgag acgcacatga
     4081 cctggcatcc cgcaaacccc atccgccatt cgctccccgg cgcgcgctgg ccgacccgcg
     4141 cagaggccgc ccgcgcgctc ctctttcaac cggtagacat cggctcgctc acgctggagc
     4201 aacgcacctg ggttccggcg atggttccgt ggcgcgcgac ggaagacggt tttgtgacgc
     4261 gcgaagtgct cgaatggtac gggcgtttcg cggcggggcg accgggcgca atcgtcgtcg
     4321 aagcgacggg cgtgcgcgac atccccagtg gaccgctctt gcgcatcggc cacgaacgct
     4381 ttctgcccgg cctgcgcgaa ctcgtcgaaa ccgtgcgccg cgcgagcgac ggccacacgc
     4441 gtctcttcat acagatcata gatttcctct cggtcaaacg ccgtcctgaa aagtcgaaat
     4501 acttcgcgcg gtttctacag ttagaagaac gccacgcacg cgcgctcgcc gccgcaacag
     4561 gtgacgagag ttggcttcgc gccggggaga cggagacgcg cgccttcctc gccgccgcgc
     4621 ccgaagagtt gctagggaaa gttctggacg agcgcgaact cgaatcgctc acgcgcggct
     4681 accgcgaacg cgtgaccgac atgcacctcg cgcacgtcga agcgttgccg cgcgtgctgc
     4741 ccgaaatttt cgccgccgcc gcgcgccgcg cacgcgaggc cgggttcgac ggcgtcgaac
     4801 tccactacgc acacgcctac acgatggcct ctttcctctc cgcgctcaac acgcgtccgg
     4861 acggctacgg cggctcgcgt gaaaatcgtg tgcgcttgcc gctcgaagtt tacagggcgg
     4921 tgcgcgaacg agcggggcgg gactatacgg tcggcgtgcg ctttctcggc gacgaagtga
     4981 tcgcgggcgg gagcaggctt gaagatgcga tctatttcgg gcgcgagttc gcgcgggcgg
     5041 gcttcgattt tctctcgctc tcgaagggtg ggaagtttga agacgcggcg cagccgaagg
     5101 tcggccatgc cgtctatcct tacacgggcg agagcggcta cgagtgcatg ccgaccattc
     5161 gctcggacga gcgtggtcct ttcggtcgca acgtctcgct cgccgccgcc gtccggcgcg
     5221 cgttgctcga tgaaggatcg cacacgcccg tcgtcgtcgc cggaggcatc tgcaccttcg
     5281 agcaggccga agagattttg cggcgcggcg aggccgacat catcggagcc gcacgccaga
     5341 cgctcgcaga ccccgactgg tttctcaaaa cacggctcgg caaaggcgac gaaattcgtc
     5401 gctgcaccta caccaactac tgcgaaggct tagaccaggc gcacaaacag gtcacctgca
     5461 aactctggga tcgagtgcaa ctcgacgaac cgaacatctc actcagccac gaccgccgcc
     5521 gacgcctgct cgcgcctgat tggaaggtag aagaggacgg ggaaaagggg acagggtaag
     5581 gtaggtttgc tttagcccgt ttagggatta gatgtcgccc cgcacgaggc tttaatccga
     5641 cgcggacttg attgctgcct catacgcttc aatgaatgcg ggatcgcgcc gtttcaagtt
     5701 ctcatagtcg atcttcttgc tgcgcgtcat caacgaatag gcgaagggca ggggcgcgag
     5761 gtgcatgtag tcgcgcgcgt gctcaaacca gacgaggctc tcatacgcgg cctgttgata
     5821 ctcttcgatg acgggcttgc gcacgcgctc aaactctgcg agcgcacccc gcacgttcga
     5881 gttggcggcc aagcaacgat acagcgcgat agcatcttcg agggcgagct tcgtgcccga
     5941 gccgatggag aagtgcgccg tgtgcaaagc gtcgccgagg aggacgacgt tttcgtgttg
     6001 ccaatgccgg ttcttcacca gcaggaagtt gatccacttc gaattgtttg agagcaaagc
     6061 atgcccgccc aagtctctcg cgaagacggc ttccaggtaa gcgcgcgtct ccgcctcggt
     6121 catctccgca aacttcgcgc ccgcccacgt ctccgcgtcg cactcgacga tgaaagtgct
     6181 cgtctgcggg tcgaacttgt aagagtgagc ggcgaagacg cccgccgccg attcgcgaaa
     6241 ggtcagtgtc agcccgtgaa agagttgatg cgtgccgtac cagatatact tgttgtgccg
     6301 cacgccgagc gaaggctcga acgagtcgcg gtaacgctcg cgcacggtgc tgttcacgcc
     6361 gtccgcgccg acgatcaggt cgcagccgga aaactcttcg aggttcccga cctcggcacg
     6421 aaaacgcaac tcgacgccga gcgcgcggca gcgcgcctgc aagatgttga gcaacttgat
     6481 gcgcgcgatt ccggaaaact tgttgccccg gatcgtgacg cgctcgtcgc gatgcaccac
     6541 gtccacgttg tcccacgtcg caaagctgcg cgtgatctcg cggtaggttt cctcgtccgt
     6601 gtcgcgcaaa tacgagagcg tcttatccga gaagacgacg ccccacccga aagtatcgtc
     6661 gggcgcattg cgctccaaca ccgtgatctc ataagacggc tcatgcttct tcatcagaag
     6721 cgcgaagtac aacccggctg gcccaccgcc gatgatgaca attttcatag cttgcccgcg
     6781 ttcatccgct ttcaggtaag tctcacgcta ttgtagcgtc tcggcgcggg ctgacaagtt
     6841 gagaaacgat cagcgcggat tcgcctcctg ctctcgctgc cgcgctgcca agtcgtcctc
     6901 tggtgtgttg cacgggtagg ttggcgcggt tggcgacggt cggttactta gtatccaatt
     6961 tacgttaact ttatacatct tcaagtcaca cacattgaaa gctgttggaa agagcacgag
     7021 cgcgactagc aggagcgtaa aaaaactgac tgccagcgca tgacgtaagg tgcggactcc
     7081 gggcggctga aagccgagaa actccgacat ataaataatc tcccggttga ttattttaag
     7141 cgaccggaac gccagcagaa tcaggagcgc gaaggagaga ttcataaaga acggaaaagc
     7201 aaatgaccat ctgactaagt tttgctcgcc ggattgaagg gaaaaagaca ggatcgctcc
     7261 ggtgatggca aagttgagaa agttgaaccg gatcagcagg tcgagatact tctcataaag
     7321 cctgatctgc atttgatact gctccttgag cagctcaacg ttgatgccac ttacgatctg
     7381 cggagtctcc ggcggggacg caaacgacaa aaagcgtttg tgcttctccc ttaaacgctc
     7441 ctcccacgat tcgtaatacc ggaggtgctt cgggatacag atgaacatgt ccagcgaaaa
     7501 ccacttgttt tcaaactcct tcggaccaaa gaaccttgcc cgagaaaacc tcctggtctt
     7561 caacttgtgc tgtctaatat cctcacggct cggcggataa tcgcaatggt aataagccgg
     7621 gtatcgccca tgccatatct cactaaagcg aatgcataga aagagactct ttagaggaaa
     7681 gagaagcaat agaagaaagc caagccagag aagaaagtcg aaccagagaa gacggtcaag
     7741 ccagagaaga cgggtaaacc agagaagaaa gccaagccag agaagacggg caaaccagag
     7801 aagaaagcca agccagagaa gacggtcaag ccatcggaca atgcgaagcc aaagagattt
     7861 tggccgctgc tgggattcgt tagattccgg tttgacggaa ggcggcaggt ctttttcctg
     7921 atccattggc gaggactcct atcacggtga aggaacgtga gcgagcaagg atgcggcttg
     7981 aacttgcgaa cggggctgcg cggcgaatac tactctttca acaattctct ggcaatcact
     8041 aacttctgaa tctcggacgt gccttcgtaa attctgagcg cgcggatgtc tcggtagagg
     8101 cgttcgacga tgtggccgtg gacgagtcct gcgccgccgt gaatctggag ggcttggtcg
     8161 atgatgcgct gcgcggcttc ggtggcgtag agtttggcgg tggaggctgc gcgcgagagc
     8221 gtgggcgcgt aggtgtcttc ctgcccgtag gtgtctctga ggtaagcgga gcgatagacg
     8281 agcaggcgcg cggcgtcgag ttcggcggac atgtccgcga gtttctcctg aatcaactgg
     8341 tgcgcggcga gcggttgccc gaactgcgtg cgccgacgcg cgtaagcgat ggcttcgtcg
     8401 agcgcgcgac gcgccatgcc gcaggccgcc gcgccgaccg atgcgcgaaa cgtgccgagc
     8461 gtcagcatgg cgaggcgaaa gccttcgccc tcctctccca cgcggtcttc tgcgggcacg
     8521 cgcaagttgt tgaactcgat ctcgccaatc ggatgcggcg agatgagacg cgtccgttcg
     8581 cgcaccgtga agccgttcat gcgcgcgctg acgacgaagg cggaaatctc cgcgcgcccg
     8641 tcgggacgac tgcccgtgcg cgcgaagacc gtatagaaat ctgcgatccc tgcgttggag
     8701 ataaaacgct tgcgcccgtc gagcacgtag ctgtcgccgt cgcgccgcgc ggtggtctgg
     8761 atggcggaga tgtccgagcc gacctccggt tcagtcaaag cgaaagcggc gacggcacgc
     8821 cccgtcgcgg cgcgcgtgag ccagaagtcg cgcacgtgtt cgggggcggc gaggctgacc
     8881 gcgtgcgtgc cgagtccctg catcacgaag gcgagatcgg cgagcggcga actgtaggaa
     8941 agctcttcgc gaatcaggca gagcgcgcgc acatcgaacg gcgcatgcgg cctcgccacc
     9001 gcataacgca gcaactcggc ctgcgccagc agcgacacca actcgcgaaa cttctgatcc
     9061 gactgcgcct gatccgtctc ttcctcatca ccctccgcgc gcggctcgat ctcgcgccac
     9121 acgaaactgg cgacgctcgc cgcgagccgc aaatgctccg gggtgaaaaa cggcgtctct
     9181 tcgataaacc gatccataaa aatagtgatg agtgatgagt gatgagtgat gagtaaaaac
     9241 aaaagagcgt tgtttttagc tcatcactca ttactcatta ctcattactc attactccct
     9301 taaaaactgg ctcgcgcttc gcgttgaagg cttcgtgggc ttcgcggaag tcggggtgtt
     9361 gcatgcagac ggattgcgcc tgcgcttccc attcgagcgc ggtgtcgaga tcgacgtgca
     9421 tttctcgatt gagcatctct ttggtcatgc cgagcgcgaa ggcgggacca cgcgccagcc
     9481 tcccggcgag ggcgcgcgtc tccgcttcga gttcggcagc gggcacgacg cggttataga
     9541 gtccgatgcg ttcggcctct gcggcggaga tgaagtcgcc cgtgtagagc aactcggtcg
     9601 ccttcgccag cccgacgtag cgcggcaaca ggtaggccgc gcccatgtcc gcgcccgaca
     9661 gcccgacgcg cacgaagaga aacgcgatgc gcgcctccat cgctgcgatg cgcagatcgg
     9721 ctgcgagcgc gacgaccgcc cccgcgcccg ccgctgcgcc gttcaaactc gcgatgacag
     9781 gcttgcgcag cacgcgcatg ttgcgtatga gttcgcaggt gagacgcgtg aaggcgaggt
     9841 ggccttgaat gtctagcgat tgcaggtcgg cgatgatgtc gcgcacgtcg ccgcccgaac
     9901 agaacgcgcg ccccgcgccc gtgatgacga cggcgcgtac gtccgcttca tctgcgaggg
     9961 cggcgaacgt gtcggtcaac tcgcggtaga cttcaaaggt cagcgcgttc agacgctccg
    10021 ggcggttgag cgtgatccac gccacgccgt ccgcgcgcac ttcgtaaaga aaactcttcg
    10081 gagaaagcat aagagagcag ggttgagtga taagtgacgt gtgacgagtt aaaagaaacg
    10141 cgcgacggtg aacgacatgg cgacgagtga gcaatgacga gccggaacga gtctttataa
    10201 cttttcaccc gtcacttgtc actcgtcacg agaacaagtt tttgtcttca acttgtcact
    10261 cgtcatttgt cagccgtcac tgctcttatg acggcgatgc cttcgatctc cactttcgct
    10321 tcgtcttcga gcagggcttt gacttcgacg agcgccatgg cggggaagtg gcgtcccaga
    10381 agtttgcgcc agcacgcgcc gatctcgctt tgacgcgagc ggtattcctc tttgtctgtg
    10441 acgtagatac agagacgcgc gatctgttcg ggcgcgccgc ccgcctcgct gacgaccgcg
    10501 agcacattca tgagcgcgcg ttcaaactgc gcgacgaaat ccgtgctcac gatctgctgt
    10561 cgttcgtccc aagctatctg cccggcgatg aaaagcagcg cgccgccctc cggcgcaagc
    10621 acgccgttcg cgtagccgcg cggccttccc agagacgcgg ggttgatcaa tttaaacata
    10681 gctgtcagcg atgaactgtt agcagagaca ggaaccggcg gctgccgttc gatagctgct
    10741 ggctaacaac tcatcgccaa caaccgacag cctatcttaa gagactacgc taccgccatc
    10801 gacgttgatg gcctgaccgt tgatgccgcg cccgtcttcg gaggcgagca gcagggcgag
    10861 cgcggcgacc tcttccggcg tgacgaggcg gttttgcgga ctcatacgct tgatggcttc
    10921 gagcgcgtcc gccgtggtgc gcccggtctt cgcttcaatc tgttcgagcg cgcgcgtcgt
    10981 catgtccgta tcgacatagc cgggacagat ggcgttgacc gtcacgcctt tcgccgcgac
    11041 ttcgagcgcg acggaacgcg tgaggccgac gacgccatgc ttcgacgccg cataagccgc
    11101 gatgtaaggc gcacccgtct tgcccgcgat ggacgcgatg ttgatgacgc gcccccaacc
    11161 gcgttccagc attgcgggca gggcggcgcg cgtgcagtag aaagtggcgt cgaggttgat
    11221 ggcgaggtgg cgtcgccagt gcgcgtcgtc ggtgcgcgtg atgggcgcgc tctcggcgat
    11281 ccctgcgttg ttgacgagaa tgtcggcatg gcgtccgaag ccgttgtaag tggcgaggaa
    11341 aaagttcgcc acatcctttg gatcagacac atcgcacacg tcgttcaaga catctacacg
    11401 aaactccctg cgaatttcat ctgccaccgc ttcgatttcg ctccccgtgc gcgagcagag
    11461 cgcgaggctt gcgcccgcac gcgcaaacgc gaacgcgatg gcgcgcccga tgccgcgacc
    11521 gccgcccgtg ataagtgcga ttttaccttt aagctccaag tctcaagtcc cacattccga
    11581 gtcacgaatc tcaagtctca cagcctctcc cgacttgaga cttacttgct aaagcgcggc
    11641 gcgcgtttct ctaaaaacgc acggacgcct tcgcgtgcat cggtcgtctg aaagcagcgg
    11701 agttgcgcct cggtttcgta ttgcagcatc tcttcgagtt cggcgtgatg gctcatgtag
    11761 acggcgtgct tggcggcggc gattgattcg ggcggggcat cgcgcaagcg ttcggcgagc
    11821 cgacgcgtct ctgcggccag ttgttcgtcc ggcacgacgc ggttgacgag tccgagcgtg
    11881 agcgcacgcg cagagtcaat cgcgtcgccg agaaaaaaca tctcgcaggc gatgtttgaa
    11941 ggaacgatgc gcggcagaaa atatgtcccg ccccaatccg ggtgcaagcc cactttcaca
    12001 aacgactggc tgaagcgtgc cgactccgcc gcgatgcgca ggtcgcacgc gagcgcgaga
    12061 ttgaagcccg cgcccgaagc cgtgccgtta atggaagcca gcacgggttt gaccatcgag
    12121 cggatcgccg tcaccacgcg ccgacctgcg ccgagcatcc gcgcaaactc gtctgcgtcc
    12181 tcgcgctcca tcatctcatc catcgccgcc acgtcgccgc ccgcgcagaa ggcacgcccc
    12241 gcgcccgtga tgactatcac gcgcacgtcg tcgtcgcttc cggcgcgctc cagagtctcg
    12301 cccagatcgc gccgcatgtg tcccgcgaaa gcgttcaact tctcggcgcg atccagcgtg
    12361 atcgtcaaga tgcgttcgcg cacctgaact ttgatgaatt gctccataaa aacagtgaca
    12421 agtgacaaga taagtgatga gtgatgggtg atgagtgatg agtgaaagat aaagttttat
    12481 gttttaactc gtcacctgtc actcgtcact tgtcactctc ttatcgtcct ttgaattgtg
    12541 gtttgcgctt gccgacgtag gcgtcgagtc cttcgcgcgc gtcttcggat tggaagagtt
    12601 gttgttggag ttcgcgttcg agcgcgagcg cggactcgaa gggaatctcc gcgccggttt
    12661 gcaccgcgcg cttgatgcgc ccgacggcgt gcgcggcctt gtccggcagc gtgaacttcg
    12721 cggcataggc caacacctgt tgcatgaacg ccgcgcccga ctccgcttcg tagatatgat
    12781 tgacgaggcc gagttccgcg ccccgctcga agtcgaagag ttcgcccgtc gtcatcaatt
    12841 cgatggcgcg tgatttaccg accatccgca cgagtcgctg cgtaccgccc gtgcccggca
    12901 gcacgccgag cgaaacttcg ggcaagccca tcctgcccgc gccgcgtcga gcgatgcgga
    12961 tgtcagccgc catcgccact tcgaggccgc cgccgacgca gtggccgttg atggccgcga
    13021 tgacgagttt cggcgtctgc tccagacgcg acagggtttc gttcgcgtga agacagaaat
    13081 agtatttaaa ggtcggcgtc acgctctgga gcatgccgat gttcgcgccc gcgcagaaaa
    13141 acttctcgcc gctcccggtg agcacgatga cctgtaccga ctcgtccatc cgcgccctga
    13201 gaatcgcgcg gtcgagttcc cccatcatct catagctgta cgtgttggcc ggaggatcgt
    13261 ttagggtcaa caacgcgacg ccgtcttcag cgcgataccc gacgagcgtg ccgaccgcct
    13321 cctccgtccc ctgcatcttc gcttccgctt ctaccatctc aatactccac tgaaaaaaga
    13381 tttatgtatt tgtgttacgc cacacgctga ggcgttccgc cgccgcaccg gtcttgcgtg
    13441 tgaactgtag ttcgtgcccg gcctgtccgg caaggaagcc ggagaggaag aggcgcgtga
    13501 gattgtcaac gagatcgccc acggagagtt tgccgcgcgg gtcgtaccaa ttgtagatcc
    13561 agttcatcat gccgaacaga gcgtaggtgg cgacggtgag gtcgagtttc tgcaccccgc
    13621 tctcgtggcc ttcgcgcgcc tgttgcgctt gctgcacttc cgagagaatg cgacggacga
    13681 ggcgcgtgta ctgttgcttg cgcccggtga cgtgcgcgtg catctcgccc gcgagcgagt
    13741 cggcttcgtg cgagagcact ttcatctcgg ccatgttggc ggcgaagaac gagaggtgat
    13801 tttcgatgac gagacggagc ttggcgaccg catccgtcgt ctcgcgcaga cgctcttgca
    13861 gacgttcgtg gacgcgcccg aagcaattgt cctgaatcag gaagagcagt tcttctttcg
    13921 acttgcagta ataataaagc ccggcgaggc tcacttccgt cgcgcgtgcg atgtcgcgca
    13981 tcgaggtcga gtgataaccc ttctccgcga agatgcgcgc gctcgtgcgc aggatgaact
    14041 ccagtttctg atcgtaacgc gagaagtctg tcgaaagatt agaggccata aaaacttgga
    14101 gacggaattc agaatacaga agtcagaaga agaagaacca aatcattcct gccttccgac
    14161 tcctgtgttc tgttttctga attctgcatt tagagtcgca cgtactcgaa ctcgaatggc
    14221 ttgcccttga tcccggcggc gggcggcgcg atccagttgg cgattttgcc gggttcggtg
    14281 acggggtgca tgaggctgcg gacgaactcg cggtcggcca
//
LOCUS       DASWNS010000611        16367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_49524, whole genome shotgun sequence.
ACCESSION   DASWNS010000611 DASWNS010000000
VERSION     DASWNS010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16367
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49524"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGB76_20925"
     CDS             complement(<1..192)
                     /locus_tag="VGB76_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101401.1"
                     /translation="MPKRTDINKILVIGSGPIVIGQAAEFDYSGTQACRVLRQEGYEV
                     VLVNSNPATIMTDPEFADST"
     gene            309..1475
                     /locus_tag="VGB76_20930"
     CDS             309..1475
                     /locus_tag="VGB76_20930"
                     /inference="COORDINATES: protein motif:HMM:NF024764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101402.1"
                     /translation="MRRTFCLMLCLLFAASTSPAQNPKTAEDFYNRGLQKQAQQDLEG
                     ALADYDQALALKPKKAETIATIYLNRANVRMAQGDMKAALLDLNQALAVNPREFLALY
                     NRGVVHMDLGQFDEAIADFDATLAIQTNFAMAFNNRGNARKAKGDIEGAIADYDNAIR
                     HYPQGELGYFNRAAARQSRNEFDKALADYTQAISIAPQLVEAYFNRGIVHQLMRNLDA
                     ALADFNKAIELKADDADLYVARGNALQARGESDKAIADFTKALALNPQHADAYTNRGI
                     ARKIRKDANGALADFEQAIAANPRDAVAYVNRGTLREERGDTKGALADYNMAIAINPA
                     LAQAYANRGVIRLQQGKVEGAEQDFAQAVALDGTLKPSLEKYLAEMKRHRAKKP"
     gene            complement(1508..2332)
                     /locus_tag="VGB76_20935"
     CDS             complement(1508..2332)
                     /locus_tag="VGB76_20935"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0101403.1"
                     /translation="MSSDNAKKIPPLRAVPEKDTGTPALPFDSSPADDGALNEASPSA
                     CAYCYGSGMEVVAGKGARRCRCREQGGRTKMQESARIPRRYDGCSLQNYFPAPNNGTQ
                     LKAFNYAFRLVREYPAVDRGLLFMGTVGVGKTHLSVAILRGLMEKGVPCLFYEFGSLL
                     KEIQDSYNKISHTSELGLLAPVYQAEVLVLDELGASKPTDWVRDTMMQIIGTRYNDKK
                     LTIFTTNYLDARRAATDETLEDRVGVRLRSRLFEMCKTVHIEGEDYRRRFDEQQKP"
     gene            complement(2591..2752)
                     /locus_tag="VGB76_20940"
     CDS             complement(2591..2752)
                     /locus_tag="VGB76_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101404.1"
                     /translation="MAKCPYCKRPLAGLGLNCRACRRYVLGWPHVLLLSLVILISLAV
                     LLELMLHIL"
     gene            2977..3432
                     /locus_tag="VGB76_20945"
     CDS             2977..3432
                     /locus_tag="VGB76_20945"
                     /inference="COORDINATES: protein motif:HMM:NF042704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-phosphate processing receiver
                     domain-containing protein"
                     /protein_id="HEY0101405.1"
                     /translation="MSIWQGLLGKLGLLDASRQRGAIRVFLLDDDEKRHDWFAERFNG
                     DQLDIAVEPRRAIDLLSNYFYDAIFLDHDLLPEHYKSSAHDDERTGYAVAAWLAAHPQ
                     VQPAATILVHTRNADGAMRMVEEMRRAGRRAEYVPFPLLSQQIKHYWQR"
     gene            3576..4724
                     /locus_tag="VGB76_20950"
     CDS             3576..4724
                     /locus_tag="VGB76_20950"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0101406.1"
                     /translation="MNRPRVKAAFAFVDKDRAATLREWSAITEINAPSGKERPRAQYI
                     EKLLRGYKMSSVRYDATGNLIAERKGTGGGRPRIVFDAHLDTVFQEGLKIKTVIRDGR
                     IYAPGVGDDTRNIEALLAMIRALDAAQIKTRGDLIFVFTVEEETALRGAEEYVKENKD
                     RIDHYIALDGGYEGFTYGGIGINWYKHHFIGPGGHTRSRTPPYSATLPLARAIARIYE
                     LEVPRTPSSNLNIGMLGGSEVVNAKAADAWFTVDLRSTDNEVIADLEKRINAIIQEEA
                     KRAGMTVKTELISSSPSAQIPGHRTSPLVRTAEAVHLAMGFENPPITITGSNNSSAAL
                     LAGVSSISTGAGPCGDSHALTEYCEIEPFYKGIKKIILLGLALAGTND"
     gene            complement(4793..5311)
                     /locus_tag="VGB76_20955"
     CDS             complement(4793..5311)
                     /locus_tag="VGB76_20955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101407.1"
                     /translation="MSQPSIQFGPKANQAVVSVHTLGVLTEILNAANLNSCLITSTSR
                     TPADQARIMFNNIVREGVVKQKALYKAPGKAVVDEYVKAKNAGKNSDQIKAAMEAKIR
                     SLGPASVSRHCADPAILNVVDIAPSSLANKLAFDKATKAAVTKGTVSKFLTPADNDPA
                     FHLEIPQPKAPA"
     gene            complement(5408..6415)
                     /locus_tag="VGB76_20960"
     CDS             complement(5408..6415)
                     /locus_tag="VGB76_20960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY0101408.1"
                     /translation="MISSLWQLGGLGWKELAKRVWSEISEDDIFGRSAQLSYYFLLAL
                     FPLLLFITAVLGQFAEAGSELRQTLLNLLGSVVPQEAGDLIHETIDKVEEGSGGGKIS
                     FGILATLWAASNGMTAICQTLNVAYEVEETRPWWKVRLVSLALTVALAVLILFALTLM
                     LFGGHIAEYIAASFSFGQVFTWAWKILQWPVVLIFVLVAFGLIYYLAPDVKDRSWHWV
                     SPGAAIAVALWLVVSFGFRTYLGYFNSYNATYGSLGAVIILMLWFYFTGAAILIGGEV
                     NSEIELATTGKDEKQIDPGAAKPSSPATTAGQQTSGAKTPGELAAAARRHEKERRTKS
                     R"
     gene            complement(6472..7455)
                     /gene="lepB"
                     /locus_tag="VGB76_20965"
     CDS             complement(6472..7455)
                     /gene="lepB"
                     /locus_tag="VGB76_20965"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY0101409.1"
                     /translation="MSNDSRSTIIEEPPLETPEGKGARRSSAAVTERDAEQERARGGA
                     SGGAAKPVQPGPARSVWREYFESLVVTAIMALFGMTFVVQAVKVPTGSMQNTITIGDH
                     LLVNKFIFAPGTSPLPFLPQREIRRGDIVVFKYPGNQIEPWRDDPSDPGNRQYITNYV
                     KRVIGLPGDRIELRGTSVIINGQKLNERVITAINHPDNPRTPDRREDHAPLEMEANPP
                     PEGDGTYTVAYSPESRQAGEADAESFPIYRTLNGQTVITVPPDSYFVMGDNRDNSADS
                     RYWGFVQRDLIIGRALFVYWSYDESQPSGGVPFIQDFFSNTRWSRTGTMIK"
     gene            complement(7519..8259)
                     /gene="rnc"
                     /locus_tag="VGB76_20970"
     CDS             complement(7519..8259)
                     /gene="rnc"
                     /locus_tag="VGB76_20970"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY0101410.1"
                     /translation="MADTDLGALEAKLGYAFNDRTLLERALTHRSWAHEHVAPGEELE
                     ARRLHNEALEFVGDSVLGLAVADYLFQSTADASEGDLSRMKHRLVSTVMLARVARRLD
                     LGNYLRIGRGEEKTGGRRKRALLADVVEAVIAAVYLDGGYGSAQEFVQHLLSSELEEA
                     TPEDAAAADYKTMLQECVQASFRVTPTYQLVETEGPPHRRTFHVEAVFAEHSVRGAGA
                     TIKAAEMDAACRAIEQLNLTLPQAEAAD"
     gene            complement(8421..9446)
                     /locus_tag="VGB76_20975"
     CDS             complement(8421..9446)
                     /locus_tag="VGB76_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101411.1"
                     /translation="MKSIRKTLNAAPHVKWRAPRSLSLVLFACLGLLVSPSGAAAQQP
                     SRQRQAARVRQQQITQAQADLYERWRANINLNQQTAFEAGREYLGKYPNDEYAARVSG
                     WVAAYEQAARKVQFENLLFKEKKYAAAYALGKQVLASEPGDLRTIINLASAGYLASNT
                     GDTSHNAETLAFARRATEMLEQGGKPSDWKPFTGQPDALAYLDFVTGELVLKETPAES
                     IRYFRKAIASEGAVKHTPVIYSRLAAAYAASEYEPQSKEFEEKFGGKEATPESQAALE
                     QINATVDRITDALARAVALSGTETQYAEARARWMQELTRFYKFRHNDSTEGLEAYIAG
                     ATAKPLP"
     gene            complement(9640..10842)
                     /locus_tag="VGB76_20980"
     CDS             complement(9640..10842)
                     /locus_tag="VGB76_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101412.1"
                     /translation="MKKLIRSLTLAAFALSAVALPAFAQTTAAPAAAPAAPANPCEEA
                     ARGEMYTKYYELKGKKDAKGQPDADAQKEAYGVGQEYLAKYASCNDTYSKAVQKFVTQ
                     YGEAVRKVGNAAEFSDALYKNKDYAKAFQLGNQMLAAEPENLSLLIQLGYAGYTAQSL
                     TPAVTTFSPTSLGYAKKAVEMLEAGKTPSEWVLFKSKDEALGYLSFAIGEYMINSDPA
                     GALPYYLKAASIAPTKDIALTYGRLGWAYQAGQYKPMQEAFTAKYKEETEESKHALNN
                     LNQVMDRVMDAYARAIRLAGTDPKFQKAKAEWMQQLTDFYKFRNNNTTTGLEAFIAST
                     ESKPLLSPFTPQPYVPAATPAPATGGTASEGGNGNGATASAPATTAPAATTQDATAAK
                     PKPATKKP"
     gene            complement(11206..12864)
                     /locus_tag="VGB76_20985"
     CDS             complement(11206..12864)
                     /locus_tag="VGB76_20985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0101413.1"
                     /translation="MIRMPHPPSPFLVSLILTLCVAVSGFAAAKKAKAKPAAAPAKSS
                     KTAKSAKAEPKSKSARGRDKTAATKKPSAKETRAERAAAQREARAERGRGGKLSKRER
                     LAQARREEAARRAEAARRAEAARRAEIARQAAIARQRAFDKAIRDEVQANILRDDATG
                     EDPEVRRVAVQALGYHAASVVVMNPRTGQVYTIVNQEWGVRRGFKPCSTIKLISGLAG
                     VVEGVIDPMIENAAYSSNRMNLTDALAYSHNGYFQQVGRRVGFERLSSYARQLGYGER
                     TGINHANEYAGRVPLFKNDVGMLRMSSHGDDFEVTPLQLATLASVFGNGGTLVTPHLP
                     RTPQEAVKFKTEVRRQLNIPQEALRRMLPGMIGAVNYGTGRKAQDSQQTIAGKTGTCI
                     GAHGVWDGSFTSYAPVEDPQLAIAVIGRGPDARRGFPASVAGQIYRALAYRFAKRNGK
                     SPIQLTPELLAPRPKVDPTTLADTEAQEDSDAAEVNSNGEEANGASNSNGVKRVLLPV
                     ETRPTGITTRPAASPANAPLNAPGKSTPTPQTDTRPRRVLTNQP"
     gene            13283..14809
                     /locus_tag="VGB76_20990"
     CDS             13283..14809
                     /locus_tag="VGB76_20990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0101414.1"
                     /translation="MSLFNVLNSFRVRLLLVLAGLLIATLGLQFYLNRRAELRVARTI
                     AEQERALAAGISLAAESMPTTTRMGILDRERQFLLREKHQGRVTNVLIVNDEGRVPGR
                     LDDDDLIDDSLDPEFVPVRLEDGTIRYPRLAAARLPRLINADQSEVSQPVAGEPRTFV
                     ISAATDEGLNYIVVVLGSAQIHDKHSLWEDARPLLPTLAVLLIATLAAAILVWRFTRP
                     IQELSDAARRVAVGEFNFRVPSATRRDEMGALANVFNEMITRLGGMRDLETRLNLAER
                     SAVVGRLASAIAHEIRNPLNYINLTLDHLRTSLAPNDPQKRAMVERLTTQLKAEVARI
                     NTRISEFLSYSRPAQLELRPLDLRAVIEDALRLVEVQAAESNIAISIEQAGDLPGVRG
                     DAESLRSLFTNLIINAVQAMEGNDAGGRLTITLSTNDVGRASVLIKDTGRGIAAESVS
                     QIFEPYFSTKETGTGLGLAIVKKAVDDHDGTISVTSKQGEGTTFTVTLPTTAEVTSDE
                     "
     gene            14879..16240
                     /locus_tag="VGB76_20995"
     CDS             14879..16240
                     /locus_tag="VGB76_20995"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0101415.1"
                     /translation="MARKSILVVDDERSQREILEMILSGEGYDLMTAASGEAALKIAR
                     DRRFDLVLTDLKMTGMDGIELLQRLLAYDSSIIVILLTAHGSIDSAKDALRRGAFDYL
                     EKPYDRETLLDTIRRALTKLDALDSEIISDSPEMESVKKMVLKVARSASTVLIRGESG
                     TGKELIARAIHNQSQRATEMFQAVNCAAINENLLESELFGHEKGSFTGAHAEKKGLFE
                     VADRGTLFLDEIGELDISMQAKLLRALQEREIRRVGGTRPIKIDVRVVAATNRDLLAM
                     VKDGRFRDDLYYRINVLSIDIPPLRERREDIPVLINFFLKKHTRNTSRLIRGLTPEAR
                     VVMLEYAWPGNVRQLESAIERAILLAEADLITVEDLPLEVRQEVRPTAEGAFKLPHEG
                     ISFEDFERNLILQAMERTDYNITKAAKLLGLTFRTLQYRLEKFGIKRADKKHGAEEEE
                     EEG"
BASE COUNT         3142 a         4944 c         5000 g         3281 t
ORIGIN      
        1 ggtcgagtcg gcgaactccg gatcggtcat gatcgtcgcc gggttcgagt tgacgagaac
       61 gacttcgtag ccttcctgtc tgagcacacg gcaagcctgc gtgcccgaat aatcaaactc
      121 ggcggcctgc ccgatgacga taggccccga accgatgacc aagattttgt tgatgtccgt
      181 gcgtttcggc atttacgatt tcgcggcgtg ctgacttaat attgaattgt ttcgttcaga
      241 tgaatcgggt cattatacat aatggctctt gagagcacga acccatctct tcaaggcgga
      301 gttgtgatat gcgacgcact ttctgtctca tgctatgcct gctcttcgcg gcttccacat
      361 ctccggcgca aaaccctaag acggcggaag acttttacaa tcgcggcttg cagaaacagg
      421 cgcagcaaga cctcgaaggc gcgctcgccg attacgatca ggcgctcgcg ctcaagccca
      481 aaaaagcgga aaccattgcc accatctatc tcaaccgcgc caacgtgagg atggcgcagg
      541 gcgacatgaa ggccgcgctg ctcgatctaa atcaggcgtt ggcggtcaac ccgcgcgaat
      601 tcctcgctct gtataatcgc ggcgtcgtcc acatggactt ggggcagttc gacgaagcca
      661 tcgccgactt cgacgccacg ctcgccatcc aaaccaactt cgcgatggcc ttcaataatc
      721 gcggcaatgc gcgcaaagcc aaaggcgaca tcgaaggcgc gattgccgat tacgacaacg
      781 caatcagaca ctacccgcaa ggcgagttgg gttatttcaa ccgggcggcg gcgcgccaga
      841 gcagaaacga attcgacaag gcgctcgccg attacacgca ggccatttcg attgcgcctc
      901 aactcgtcga agcctatttc aatcgcggca tcgttcacca gttgatgcgc aacctcgatg
      961 ccgcgctcgc agacttcaac aaggccatcg aactcaaggc cgacgatgca gacctctacg
     1021 tcgcacgcgg caacgccctg caagccagag gcgagagtga taaagccatc gccgatttca
     1081 ccaaagccct cgcactcaac ccgcagcacg ccgacgccta caccaatcgc ggcatcgcac
     1141 gcaagatcag gaaagatgct aacggcgcgc tcgcggactt tgaacaggcc atcgcggcca
     1201 atccgcgcga tgccgtcgct tacgttaatc gcggcacgct gcgcgaagag cgcggcgaca
     1261 caaagggcgc gctcgcagac tacaatatgg ctatcgccat caaccccgcc ctcgcgcaag
     1321 cctacgccaa tcgcggcgtg atacgtctcc aacagggcaa agtggaaggc gcggaacagg
     1381 acttcgcgca ggccgtcgcc ctcgacggca cgctcaagcc ctcgctcgaa aagtatctcg
     1441 cggagatgaa acgtcaccgc gcgaagaagc cttgatttat agagggcaga gcgtggaggc
     1501 gtgaaggtta aggcttctgc tgttcgtcga agcggcggcg gtagtcttcg ccttcgatgt
     1561 ggacggtctt gcacatctcg aagaggcgcg agcggaggcg tacgccgacg cggtcttcga
     1621 gcgtctcgtc ggtggcggcg cggcgcgcgt cgagatagtt ggtggtgaag atggtgagtt
     1681 ttttgtcgtt gtagcgcgtg ccgatgatct gcatcatggt atcgcgcacc cagtcggtcg
     1741 gtttcgacgc gccgagttcg tccagcacca gcacctccgc ttgatagacc ggggcgagca
     1801 ggccgagttc ggacgtgtgc gagattttgt tgtacgaatc ctgtatctct ttcagcagcg
     1861 agccgaattc gtagaagaga cacggcacgc ccttttccat caacccgcgc aggatggcga
     1921 ccgagaggtg cgtcttgccc acgcctaccg tgcccatgaa cagcaacccg cgatccaccg
     1981 ccggatattc gcgcacgaga cgaaaggcgt agttgaaggc tttgagttgc gtgccgttgt
     2041 tgggcgcggg gaaatagttt tggagcgagc agccatcgta acgacgcgga atgcgtgcgc
     2101 tctcctgcat cttcgtccgt ccaccctgct cacggcaacg gcaacgacgc gcgcccttgc
     2161 ccgccaccac ttccatcccc gaaccgtagc aataggcgca cgccgagggt gatgcttcgt
     2221 tcagagcacc atcatcggcg ggcgaactat cgaaagggag cgcgggcgta cctgtgtcct
     2281 tttcaggcac ggcgcgtagt ggtggaatct ttttggcgtt gtcagacgac attctgctcg
     2341 gcggctaaag ctgtatgtga acctttggga cgaagcggcg cggggtaaga cttggggatg
     2401 caaccgcttt ttcgaactcc tcaaaaatct tcatctccga ggtaacgtgg cgaattatag
     2461 cacccgaaac gaagattgca agaccaactt ccacgcgcca tgaaacataa caatccggcg
     2521 cgaacttcac cagttcaacc tcccgttttt cgcacgattg caataatgtc cggcagatga
     2581 gcaggcgcgt ctaaaggatg tggagcatca attcgagcaa gacggcgagc gagatgagaa
     2641 tgacgagcga gagcaggagg acatgaggcc atccgagcac gtaacggcgg cacgcgcggc
     2701 agttgaggcc gagcccggcc agaggtcttt tacagtacgg gcactttgcc atggttcgta
     2761 aaaatcaggt aaggattacc gaagcgcaca gagaggcggc ctcgtcgtga atggcagtta
     2821 aaatatcaaa tctgctgttg gctagacacg acccggctct ctcgtcgcgg ccaaagcctc
     2881 tatgctatat ttccacgcgt atactaattt catcatggag ataatgcaac acgcgctcgc
     2941 gtccgtccgg gcgcgcacgg atttgcgccg cgcctcatga gcatctggca aggactactc
     3001 ggcaagctcg gactcctcga cgcttcgcgc cagcgcggcg cgattcgcgt ctttctgctc
     3061 gacgacgacg agaagcggca cgattggttt gcggagcgtt tcaatggcga ccaactcgac
     3121 atcgccgtcg aaccgcgccg cgccatcgac ctgctctcca actatttcta cgacgccatc
     3181 ttcctcgatc acgacctgct gcccgaacat tataaatctt ccgcgcacga cgacgagcgc
     3241 acgggttatg ccgtcgccgc atggctcgcc gctcatccgc aggtgcagcc cgccgccacg
     3301 atcctcgtcc acacgcgcaa cgcggacggc gcgatgcgca tggtcgaaga gatgcgccgc
     3361 gccggacgcc gcgccgagta tgtgcccttc cccctgctct cgcagcagat caagcattac
     3421 tggcaacgct agtaaggagt tgaacttcga tgacacgcac gcctcgtcct ttaattcgcc
     3481 ttcttttagt tcgctgtgtc gcgctttttc tctgcctcgc cgtgcccgca ggggtcggac
     3541 gtgcgcagtt gaacgtggaa caggcaaagg ctctgatgaa tcgcccacgg gtcaaagcgg
     3601 ccttcgcttt tgttgataaa gatcgcgcgg cgacgctcag ggagtggagc gcgatcacgg
     3661 agatcaacgc gccttcgggc aaagaacgcc cgcgcgcaca gtatatcgag aagctactgc
     3721 gcggttacaa aatgagcagt gtccgttacg acgcgacggg caatttgatc gcggaacgca
     3781 aaggcacggg cggcggtcgc cctcgcatcg tcttcgacgc gcatctcgac acggtttttc
     3841 aggaaggctt gaagatcaag acagtgatcc gcgacggcag gatttacgcg cccggcgtcg
     3901 gcgacgacac gcgcaacatc gaagccctgc tcgccatgat ccgcgcgctc gacgcggcgc
     3961 agatcaagac gcgcggcgat ctcattttcg tcttcaccgt ggaagaggag acggctctac
     4021 gcggcgcgga ggagtacgtt aaagagaaca aagaccgcat cgaccactac atcgcgctcg
     4081 acggcggcta cgaaggtttc acttacggcg gcatcggcat caactggtac aagcaccact
     4141 tcatcggccc cggcggccac acccgctcgc gcacgccgcc ctactccgcc acccttccgc
     4201 tcgcacgcgc catcgcgcgc atctacgaac tcgaagtgcc gcgtacgcct tcttcgaatt
     4261 tgaatatcgg aatgctgggc ggctccgagg tcgtcaacgc gaaagcggct gatgcttggt
     4321 ttacggttga tctgcgttcg acggacaacg aagtgatcgc cgatctggaa aagaggatca
     4381 acgcgatcat tcaggaggag gcgaagcgcg cggggatgac ggtcaagacc gagttgattt
     4441 cgagttcgcc gtcggcgcaa attccgggac atcgcacttc cccgctcgtg cgcacagccg
     4501 aagccgtcca tctcgcgatg ggctttgaga acccgccgat cacgatcacc gggtcgaaca
     4561 attcgagcgc cgcgctgctg gcgggcgtct cttcgatctc gaccggcgca ggtccctgcg
     4621 gcgattctca cgcgctcacc gaatattgcg agatcgaacc gttctacaaa ggcatcaaga
     4681 agatcatcct gctcggactc gcgcttgcgg gcacgaacga ttagcacgag gcgaaaatcc
     4741 gtgtgcggat aaaggcgacg cgctttcagg tgacgaactt tcgagcgacc ttttaagcgg
     4801 gagctttcgg ttgcgggatt tccagatgaa acgcagggtc gttgtcggcg ggcgtgagaa
     4861 acttgctcac cgtacccttc gtcaccgccg ctttggtggc tttatcgaac gccaatttgt
     4921 tcgccagaga cgaaggcgcg atgtcaacca cgttcaggat ggccgggtcg gcgcaatgcc
     4981 tgctcacgct ggccgggccg agcgatctga tcttggcctc catcgccgct ttgatctggt
     5041 cgctgttttt gcccgcgttc ttggccttca cgtattcgtc aaccaccgct ttgccggggg
     5101 ctttgtagag cgccttttgt ttgacgacgc cttccctaac gatgttgttg aacatgatgc
     5161 gcgcctgatc cgccggagtc cggctggtgc tcgtgatcag gcacgaattg agattggcgg
     5221 cattaagaat ttccgtcagc actccgaggg tgtggacgct gacgacggct tgattcgctt
     5281 taggaccgaa ttggatagaa ggttgtgaca tagtgcgcgg ataatattca ttgtgcgcgc
     5341 gatgtcaaga attttttgcg ggctgcccgc acaaggcggc gcgaaccccg catctccgat
     5401 aaataattca ccgactcttg gtgcgtcgct ccttttcgtg ccgtcgcgcg gcagcggcaa
     5461 gttcgccggg agtctttgcg ccggacgttt gctgcccggc agtagttgcc ggagacgagg
     5521 gtttcgccgc gcccggatcg atctgcttct cgtcctttcc ggtcgtcgcc agttcgatct
     5581 ctgagttgac ttcaccgccg atcaggatcg ccgcgcccgt gaagtagaac cagagcatga
     5641 ggatgatgac cgcgccgagc gagccgtagg tggcgttgta ggagttgaaa tagccgagat
     5701 aggtgcggaa gccgaaggag acgactagcc agagcgcgac ggcgatggcg gcaccggggc
     5761 tgacccagtg ccaactgcga tctttcacgt cgggcgcgag atagtagatc agcccgaaag
     5821 cgacgagcac gaagatcagc accaccggcc actgcaaaat cttccatgcc cacgtgaaga
     5881 cctgcccgaa gctgaaactc gcggcgatgt attcggcgat gtggccgccg aagagcatga
     5941 gcgtgagcgc gaagaggatc aggacggcga gcgcaacggt taaggcgagc gagacgagcc
     6001 gcaccttcca ccaggggcgc gtctcttcaa cctcgtaggc gacgttgagc gtctggcaga
     6061 tggcggtcat gccgttggaa gccgcccaga gcgtggcgag tatgccgaag gagattttgc
     6121 cgccgccgga gccttcttct accttgtcaa tcgtctcgtg aatgaggtcg ccggcttcct
     6181 gcgggacgac tgagccgagc aggttgagga gcgtctggcg cagttcgctt ccggcctctg
     6241 caaactggcc gagcacggcg gtgatgaaga gcagcagcgg aaagagcgcg agcagaaaat
     6301 agtaagagag ttgcgcggag cgtccgaaga tgtcgtcttc acttatttcc gaccagacgc
     6361 gctttgccag ttccttccaa cccaaccctc cgagttgcca cagagaagat atcacttgtt
     6421 cctgcccctt tgcgcgcgtg ccgaggcgtg cgcgcgccct tgttcgttag tttatttgat
     6481 catcgtgccg gtacggctcc agcgcgtgtt gctgaagaag tcttgaatga aggggacgcc
     6541 gcccgaaggc tggctttcgt cgtaagacca gtagacgaag agcgcgcgac cgatgatgag
     6601 atcgcgctgg acgaaacccc agtagcgact gtccgcgctg ttgtcgcggt tgtcgcccat
     6661 gacgaagtag gaatcgggcg gcacagtgat caccgtctgc ccgttcagag ttctgtagat
     6721 gggaaaactt tcggcgtcgg cttcgcccgc ctgtctcgac tcgggactgt aagccacggt
     6781 gtatgtcccg tcgccctccg gtggcgggtt ggcttccatc tcaagcgggg cgtggtcttc
     6841 gcgccggtca ggcgtgcgcg ggttgtccgg atgattgatg gccgtgatca cgcgctcgtt
     6901 gagcttttga ccgttgatga tgacgctcgt gccgcgcagt tcgatgcggt cgccgggcag
     6961 accgatgacg cgcttgacgt agttggtgat gtactgcctg ttgcccggat cgctcggatc
     7021 gtcgcgccac ggctcaatct ggttgccggg atatttgaag acgacgatgt cgccgcgcct
     7081 gatctcgcgt tgcgggagaa acggcagcgg cgacgtaccc ggcgcgaaga tgaacttgtt
     7141 gacgagcagg tggtcgccaa tcgtgatcgt gttctgcatc gagccggtcg ggacttttac
     7201 cgcctgcacc acgaacgtca tgccgaagag agccatgatg gccgtgacga cgagcgactc
     7261 gaaatattcg cgccacacgc tgcgcgccgg tcccggttga accggcttcg ccgccccgcc
     7321 ggatgcgccg ccgcgcgcgc gctcctgttc ggcgtcacgc tccgttacag ccgcgctgct
     7381 ccggcgcgcg cccttgcctt ccggcgtctc taagggcggc tcttcgatga tcgtggaacg
     7441 ggaatcattg ctcataaaat ttatcgtaga aaaagaggcg cgggacggtc aagccgaaca
     7501 cggcttttcg cgcgcacgtc aatccgccgc ctccgcttgc ggcagcgtca aattcaattg
     7561 ttcgatggcg cggcaggcgg cgtccatttc ggcggccttg atcgtcgcgc ccgcgccgcg
     7621 cacgctgtgt tcggcgaaaa ccgcttcgac gtggaaggtg cggcgatgcg gcggcccctc
     7681 ggtttccacc aactggtaag tcggcgtgac gcgaaaactg gcctgcacgc actcctgcaa
     7741 catcgtcttg tagtcggcgg cggcggcatc ttccggcgtc gcctcctcca actcggaact
     7801 gagcaggtgt tgcacaaact cctgcgcgct cccgtagccg ccatccagat agactgccgc
     7861 gatcacggcc tcgacgacat ctgcgagcag tgcgcgcttg cgcctgccgc cggtcttctc
     7921 ttcgccgcgc ccgatgcgca ggtagttgcc gaggtctaga cgacgtgcca cgcgcgcgag
     7981 catcaccgtg ctcacgagcc ggtgcttcat gcgcgagagg tcgccttcgc tggcatcggc
     8041 ggttgattga aagaggtagt cggcgacggc gaggccgagc accgagtcgc cgacgaattc
     8101 gagagcttcg ttgtgcaggc gacgcgcttc gagttcttcc cccggcgcga cgtgctcgtg
     8161 cgcccaggag cggtgcgtga gcgcgcgctc caaaagtgtg cggtcgttga aagcgtaacc
     8221 gagctttgct tccagcgcgc cgagatcagt gtcagccatt tcgggttctt ccacagtgcg
     8281 cgcgattgta gcaaacgaaa tagagaaagg ggaaagcgga agcggagtcg gggaaagggg
     8341 aaggggtaaa ggttgaagaa agcgacgcac gacaggcgcg cctcttatct tctccctcta
     8401 ccccttccct tttccgtctg ctatggcaag ggcttcgccg tcgcgcccgc gatgtaggct
     8461 tcgagtcctt cggtcgaatc gttgtggcgg aacttgtaga agcgcgtcaa ctcctgcatc
     8521 cagcgcgcac gcgcttccgc atactgtgtc tccgtgccgc ttaaagcgac ggcgcgtgcg
     8581 agcgcgtctg tgatgcggtc aaccgtcgcg ttgatctgtt cgagcgcggc ctggctttcc
     8641 ggcgtcgctt ccttgccgcc gaacttctct tcaaactctt tcgactgcgg ttcgtactcg
     8701 ctcgcggcgt aggcggcggc gagacgcgaa tagatgacgg gcgtgtgctt gaccgcgccc
     8761 tccgaagcga tggctttgcg gaaatagcgg attgactcgg cgggcgtctc tttcaggacg
     8821 agttcgccgg tgacgaaatc gagataagcg agcgcgtcgg gctgccctgt gaagggcttc
     8881 cagtcggagg gcttgccgcc ctgttcgagc atctccgtgg cgcgacgcgc gaaggccagc
     8941 gtctctgcgt tgtgcgaagt gtcgcccgtg ttcgacgcga ggtatccggc ggacgcaagg
     9001 ttgatgatgg tacgcaagtc gcccggctcc gaggcgagaa cctgcttgcc gagcgcgtaa
     9061 gccgccgcat acttcttctc cttgaagagc aggttttcaa actgcacttt gcgcgccgcc
     9121 tgttcgtaag ccgccaccca tccgctcacg cgcgccgcat attcgtcgtt cggatattta
     9181 ccgagatact cgcgtccggc ctcgaaggcc gtctgctggt tgagattgat gttggcgcgc
     9241 cagcgttcgt agaggtcggc ctgcgcctgc gtgatctgct gctgcctgac ccgcgcggcc
     9301 tgccgctgcc tcgacggttg ctgcgccgcc gcgcccgaag gcgacacgag caggccgagg
     9361 caggcaaaga gaacgagact cagactgcga ggcgcgcgcc actttacgtg cggcgcggcg
     9421 ttcaatgttt tccggataga cttcatacca gttggatgtt cgagccacta cgaagaggat
     9481 gctttcggag aatagcttgc caaggcttct cagtttgcgt caagccgacg cgcgttcatt
     9541 ctcgatctca aaattcgttc aagcaaaaac cggacgtatc gttcaaatca cgatacgccc
     9601 ggctctctcg gttgaagacg attcctgctt gaagtcaggt tacggttttt tggtcgccgg
     9661 tttgggcttg gctgcggtcg cgtcctgcgt ggtcgcagca ggcgcggtgg tggccggtgc
     9721 gctcgccgtc gcgccgtttc cgttgccgcc ctcggacgcg gtgccgccgg ttgcaggcgc
     9781 gggcgtcgct gcgggcacgt agggctgcgg cgtgaaaggc gagagcagcg gcttcgactc
     9841 ggtgctggcg ataaacgctt ccaacccggt ggtcgtgttg ttattacgga acttgtagaa
     9901 gtccgtcaac tgctgcatcc attcggcctt ggccttctgg aatttcgggt cggtgcccgc
     9961 caggcggatg gcgcgggcgt aagcgtccat cacgcgatcc atgacctgat tgagattgtt
    10021 cagagcgtgc ttgctctctt ccgtctcttc cttgtatttc gcggtgaagg cttcctgcat
    10081 cggcttgtac tgtccggcct gataagccca gccgagacgc ccgtaggtca gcgcgatgtc
    10141 cttcgtcgga gcgatggagg cggccttgag atagtaaggc agcgcgcccg ccgggtcgct
    10201 gttaatcatg tattcgccga tggcgaaact gaggtagccg agagcttcgt ctttcgactt
    10261 gaacagcacc cattcggacg gggtcttacc ggcttcgagc atctcgacgg ccttcttggc
    10321 gtaaccgagc gaagtcgggc tgaacgtggt caccgccggc gtcaggctct gtgcggtata
    10381 accggcgtag ccgagttgaa tgagcaggct cagattttcg ggttcggcag caagcatctg
    10441 gttgccgagt tggaaggctt tcgcgtagtc cttgttctta tagagggcgt cggaaaattc
    10501 ggcggcgttg cccaccttgc gcacggcctc gccgtactgt gtgacgaact tctgcaccgc
    10561 cttcgagtag gtgtcgttgc agctggcata cttcgccaga tattcctggc ctactccgta
    10621 ggcttccttc tgggcgtcgg cgtcgggctg tcctttggcg tccttcttac ccttcaattc
    10681 ataatatttt gtgtacatct cgccgcgcgc ggcttcctcg caagggttgg ccggagcagc
    10741 gggcgcggcg gcgggcgcgg cagtggtctg cgcaaacgcg ggcagcgcga cagccgacag
    10801 cgcaaaggcg gccagtgtca atgaacggat cagttttttc atgggtcgca tgtcctccaa
    10861 acaaaaatcg tcttcgtaat tcggagcctt caagtggggg ccgcgcgaac ttcaagcgtc
    10921 gcgtgtcaat cagatgcgca ggtcgggcgg ctagttgccg tcgcgcgaga gagagcgtcg
    10981 cgcatcgggt gcgttgacct ctggggaaga gccacacctt acactctgag caaattgtgg
    11041 aatcgggtgc aacgcacagg cgttgcgccg tgatactgca ataactgacg gaaacgtgtc
    11101 aagcctctcg cgtcacccgc cagacaaaaa attacgccgc gttgtgtgtc tccattagac
    11161 acctcaacgc ggcgcattgt tccttacatt cgggttataa cttcattacg gttggtttgt
    11221 cagcacgcga cgcgggcgcg tgtcggtctg cggcgtcggc gtgctctttc ccggtgcgtt
    11281 taagggcgcg tttgcagggc tggcggcggg tcgcgtcgtg atgcccgtgg ggcgcgtctc
    11341 gacgggcaac agcacacgct tgacgccgtt tgagttcgaa gctccgttgg cctcctcgcc
    11401 gttcgaatta acttcggcgg cgtcggaatc ttcctgcgct tccgtgtcgg cgagcgtcgt
    11461 cgggtcaacc ttcgggcgcg gcgcgagaag ttccggcgtg agttggatcg gcgacttgcc
    11521 gttgcgcttg gcgaagcggt aggccagcgc gcgatagatt tggcctgcga ccgacgcggg
    11581 gaaacctctg cgcgcgtccg ggccgcgacc gatgactgcg atggcgagtt gcggatcttc
    11641 gaccggggcg taagaagtga aagagccgtc ccagacgccg tgcgcgccga tgcaggtgcc
    11701 ggtctttccg gcaatcgtct gctgcgaatc ctgcgccttg cgacccgtgc cgtagttgac
    11761 cgcaccgatc atgcccggca gcatccggcg cagagcctcc tgcgggatgt tgagttggcg
    11821 gcgcacttcc gtcttgaatt tgacggcctc ttgcggcgtg cgcggcaggt gcggcgtgac
    11881 gagcgtaccg ccgttaccga agacggaggc gagcgtggcg agttgcagcg gcgtgacctc
    11941 gaagtcgtcg ccgtgcgaac tcatgcgcag catcccgaca tcgtttttga agagcggcac
    12001 gcgtcccgcg tactcgttgg cgtggttgat gcccgtgcgc tcgccgtagc cgagttggcg
    12061 cgcgtagctc gacaggcgtt cgaagccgac gcgacggcct acctgttgaa agtaaccgtt
    12121 gtgcgaatag gcgagcgcgt cggtcagatt catgcggttg ctgctgtagg ccgcgttctc
    12181 gatcatcgga tcaatgacgc cctcgaccac tccggccaga ccggaaatga gtttgatcgt
    12241 cgagcacggc ttgaagccgc gacgcacgcc ccattcctga ttgacgatgg tatagacctg
    12301 ccccgtgcgc gggttcatca cgacgacgga ggcggcatgg tagccgagcg cctgcacggc
    12361 gacgcggcgc acttccgggt cttcgcccgt cgcgtcgtcg cgtaaaatgt tggcctgaac
    12421 ttcgtcgcgg atggccttat cgaaagcgcg ctggcgcgca atcgcggcct ggcgtgcgat
    12481 ctccgcacgg cgtgcggctt cggcgcgtcg cgcagcctcc gcacgtcgcg cagcctcttc
    12541 ccgccgcgcc tgcgcgagac gttcgcgctt cgaaagtttt cctcccctgc cgcgttcggc
    12601 gcgcgcctct ctctgcgcgg cggcacgctc ggcgcgcgtc tccttcgcgg aaggcttctt
    12661 cgtcgcagcg gtcttgtcgc ggcctctggc cgacttggac tttggctccg ccttcgcgga
    12721 cttagccgtc ttggaagatt ttgcgggagc ggcggcgggt ttggccttgg cctttttggc
    12781 ggcggcaaag ccggagacgg caacgcagag ggttaaaata agggagacga gaaaggggga
    12841 tgggggatgt ggcattcgga tcattcaaaa agtcctctcc ggttaaagct atttcgagag
    12901 cgcgcggctc ttcacccggg gtggaagtcc gcacagtttt gatggcaatg aatttgggaa
    12961 cacgtcgaga cgttattttg agcgcgagga tgggcgcaaa tcgcggcgtg tcctaatcgt
    13021 ctttgacgga aatccgtcgg ggtgaaagcg cgcttccggg agtcgtgctt ccaaacagtt
    13081 ccttcagttc aaatttacga tcttctttac tggcgtggcg gggagacaat ccccctgaag
    13141 tctccggggg gtttgcgttg gagacgcttg cgactataac atcacgaatt agtgccgggc
    13201 aacaccttta attgatttta gttagcagat ggcttctatc gttcaacttg aagcattgat
    13261 gtattaaaac atcacgtcgc agatgtcctt attcaacgtt ctgaacagtt ttcgcgtgcg
    13321 cctgctgctg gttctggccg ggttgctcat cgccacgctc ggcttgcagt tctatttgaa
    13381 tcggcgagcg gagttgcgcg ttgcccgcac catcgcggaa caggagcgcg cgctcgccgc
    13441 aggcatctcg ctcgccgccg agagcatgcc gaccacgacg cgcatgggca tcctcgaccg
    13501 cgagcgacag tttctactgc gcgaaaaaca ccaggggcgt gtgaccaatg tgctcatcgt
    13561 caacgacgag ggacgcgtgc ccggacgcct cgacgacgac gacttgattg acgacagcct
    13621 cgaccccgaa ttcgtgcccg tgcgtttgga agacgggacg atccggtatc ccagactcgc
    13681 ggcggcacgc ctgccgcgtc tcatcaatgc cgatcaatcc gaggtgtcac agccggtcgc
    13741 gggtgagccg cgcacgttcg tcatctccgc cgcaaccgac gagggactca actatatcgt
    13801 cgtcgtgctc ggttcggcgc agatacacga caagcattcg ctctgggaag acgcgcgccc
    13861 gctgttgccc acgctcgccg tcctgctcat cgccacgctc gccgccgcga tcctcgtctg
    13921 gcgtttcacg cgcccgattc aggagttgtc ggacgcggct cggcgcgtgg cggtcggcga
    13981 attcaacttc cgcgtgcctt ccgccacgcg ccgcgacgag atgggcgcac tcgccaacgt
    14041 cttcaacgag atgatcacgc ggctcggcgg gatgcgcgat ctcgaaaccc gcttgaacct
    14101 cgcggaacgc tcggcggtcg tcgggcggct cgcctccgcc atcgcgcacg agatacgcaa
    14161 cccgctcaac tacatcaatc tcacgctcga tcacctgcgc acctcgctcg cgccaaacga
    14221 cccgcagaaa cgcgcgatgg tcgaacgcct gaccacgcaa ctcaaagccg aagtcgcgcg
    14281 catcaacacg cgcatctccg agttcctcag ctattcgcgc ccggcacaat tagagttgcg
    14341 cccgctcgat ctgcgcgcgg tcatcgaaga cgccttgcgc ctcgtcgagg tgcaggccgc
    14401 cgagagcaac atcgcgatca gcattgagca ggcgggcgat ctgccgggcg tgcgcggcga
    14461 cgccgaatct ttacgttccc tcttcacaaa ccttattatc aacgcggtgc aggcgatgga
    14521 aggaaacgac gcgggcgggc gtttgacgat cacgctctcg acaaacgacg tggggcgcgc
    14581 gtctgtcctg atcaaagata cggggcgcgg catcgctgcc gaaagcgtct cccagatttt
    14641 cgaaccatat ttttccacga aggagacggg cacgggactc ggactcgcca tcgtcaagaa
    14701 agccgtggac gaccacgacg gcaccatctc cgtcaccagc aaacagggcg aaggcacgac
    14761 gtttacagtt acactaccaa caacagcaga agtgacaagt gacgagtgac aagttaaaac
    14821 aacagaactt gtcttcaact cgtcacttct cactcgtcac ttgtcactag aggtatctat
    14881 ggcacgcaaa agcatactcg tggtggacga tgagaggtcg cagcgagaga ttctggaaat
    14941 gatcctgtcg ggcgaaggct acgacttgat gaccgccgca tcgggcgagg ccgcgctcaa
    15001 gatcgcgcgc gaccgtcgct tcgatctcgt gctcaccgat ctgaagatga ccggcatgga
    15061 tggcatcgaa ctgctccaac gcctgctcgc ctacgattct tcgatcatcg tcattctact
    15121 gacggcgcac ggctcgattg actcggccaa agatgcgctc aggcgcggcg cgttcgacta
    15181 tctcgaaaag ccctacgacc gcgagacgct cctcgacacc atccgccgcg cgctcaccaa
    15241 actcgacgcg ctcgactccg agatcatctc cgattcgcct gagatggagt ccgtcaagaa
    15301 gatggtcttg aaggtcgcgc gctcggcttc gaccgtcttg atacgcggcg agtcgggcac
    15361 gggcaaggaa ctgatcgcgc gtgcgattca caatcagagc cagcgcgcga cggagatgtt
    15421 tcaggccgtc aattgcgccg ccatcaacga gaaccttttg gagtcggaac tcttcggcca
    15481 cgagaaaggc agttttacgg gcgcgcacgc cgagaagaaa ggtctgtttg aagtggccga
    15541 ccggggcacg ctcttcctcg acgaaatagg cgaactcgac atctcgatgc aggcgaaact
    15601 tttgcgcgcc ttgcaggaac gcgagatcag gcgcgtcggc ggcacgcgcc cgatcaagat
    15661 cgacgtgcgc gtcgtcgccg cgacgaaccg cgacctgctg gcgatggtca aagacggcag
    15721 gttccgcgac gacctctact atcgcatcaa cgtcctctca atcgacatcc cccctttgcg
    15781 cgagcggcgc gaagacatcc ccgtgctcat caacttcttt ttgaagaagc acacgcgcaa
    15841 cacctcgcgg ctcattcgcg gcctcacgcc cgaagcgcgc gtcgtcatgc tcgaatacgc
    15901 ctggccgggc aacgtgcgcc aactcgaatc ggccatcgag cgcgccatcc tgctcgcaga
    15961 ggccgacctg atcacggtcg aagacctgcc gctcgaagtg agacaagagg tgcggcctac
    16021 ggcggagggg gcgttcaagc tgccgcacga gggcatctcc ttcgaagact tcgagcgcaa
    16081 cctgatcctg caagcgatgg agcgcacgga ctacaacatc accaaagcgg ccaagctcct
    16141 cggcctcacc ttccgcacgc tgcaataccg gctcgaaaag ttcggcatca aacgcgccga
    16201 caagaaacac ggagcggagg aagaggaaga ggaaggttga cgaaaacccg gctcattttc
    16261 ccgccccggt tttacgccta tagacgaccg gggcgagcca actcaagaca accttcgtcc
    16321 gattaaggca accttcgtcc aacccgcgtt tttctcgttc ggaaccg
//
LOCUS       DASWNS010000612         4955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_412540, whole genome shotgun sequence.
ACCESSION   DASWNS010000612 DASWNS010000000
VERSION     DASWNS010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412540"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VGB76_21000"
     CDS             <1..333
                     /locus_tag="VGB76_21000"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0101416.1"
                     /translation="LLRYTQALMTQMSQTAVCNRLHTVEQQLCRMLLLSRDRLASDEL
                     VMTQELIANMLGVRREGVTHAAGRLQENGLISYVRGRITILDRRRLEATVCECYRVVK
                     DEYTRLLG"
     gene            800..1405
                     /locus_tag="VGB76_21005"
     CDS             800..1405
                     /locus_tag="VGB76_21005"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY0101417.1"
                     /translation="MVTKRVKKPVPQAVDRKGKDFLTEGEMKRMLDAAKGGRYGVRDY
                     LLLLMAYRHGLRVSELIDLRLKDLDLDTSRLYVRRKKGSLSTHQPIEGDELRALRAWL
                     RARSLREDARSPYLFLSERGPFTRQAVNYIVSQAGGRARLPFRVHPHMLRHSTGFYLA
                     NKGFDTRLIQDYLGHRNIAHTVKYTRTAAGRFEGLWRTGTG"
     gene            1707..3836
                     /locus_tag="VGB76_21010"
     CDS             1707..3836
                     /locus_tag="VGB76_21010"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY0101418.1"
                     /translation="MSALINLIAKVKAKDLQLGADLEREFRVLSSRLSFGLNFERHRP
                     EAVELPQRPVRRGDKVRVLPPRGSTARGDQRLWLVKAIHRDTKTAELELLDAADAETQ
                     TVALDDLIVVAEFRDTIYPGLVSTGSVSRGGDKPWHTVINGENYHVLKALTWTHRGKV
                     DAIYIDPPYNTGARDWKYNNHYVEGDDLYRHSKWLAMMERRLLVAKELLKPDDSVLIV
                     TIDEKEYLRLGLLLEQVFPEARQTMVSSLINPKGSARSDTFRRYDEYLFFVMFGKAAP
                     VSLELSDEWRVTIDRRTQTLQWASLQRKGAGGSRSSSPNLFYPVFIRNTANGPVFDSV
                     GESFFGSDWDQVTAPSGCVAVWPIRRNGSEGRWSTSPSTLRRYISLGYAQLGRWNEGG
                     TTINYLTSGEQRKIETGVFSIIARRADNSVLIDATNYKPSFVPGTQWRIKAHNSGDHG
                     ASLVAQLLPNRRFPYPKALYAVEDALRFFVANKPEAVILDFFSGSGTTAHAVMRLNKQ
                     DGGRRQCISVTNNEVGADEQKALRERGLRPGDTEWECLGICDYITKPRVQAAITGKTP
                     DGEPIKGDYKFTDEFPMSDGFEENAEFFTLTYETPVAVSYQTAFARIAPLLWLRAGSV
                     GRRIDKLPAAGWDVADAYGLLVELDKATDFLKAAREAKALRIAYIVTDDERRFQALAR
                     RLPEGVEAIRLYESYLTNFAFANGDNA"
     gene            3833..>4955
                     /locus_tag="VGB76_21015"
     CDS             3833..>4955
                     /locus_tag="VGB76_21015"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY0101419.1"
                     /translation="MKFTLHDYQGKAVRDVLNNLRKARKRWREDGDRHAFSLTAVTGA
                     GKTVMAAAVFEALFHGDDILEFDADPGAVVIWFSDDPSLNEQTRFRLMESSDRLQHTD
                     LVVVENTFNRAKFNSGKIYFLNTQKLGKKSLLVRGFEGDEDDSVFPEIRPDMRSHTIW
                     DTIRNTVEDPALTLYLVLDEAHRGMGSENREKSTIVSRLINGEKGVPGIPVVWGISAT
                     VDRFNKAMDGAHNRDTLRPVTVDTALVQESGLLKDTIVLDIPDEAGNFDTVLVRRATD
                     KLRECTKEWADYARQQRTGETVLPLMVLQVPNTPSHNDIGRTLDTIFQQWPELQETTV
                     AHVFGEHTTQTFGKYSVPYIQPQRVQDDTWVRVLIAKDAI"
BASE COUNT         1095 a         1386 c         1470 g         1004 t
ORIGIN      
        1 ctcctgcgct acacgcaggc gttgatgaca cagatgtcac aaacagccgt ctgcaatcgc
       61 ctccacacgg tagaacagca actctgccgc atgctgctgc tgagccgcga ccgcttagcc
      121 tcggatgagt tggtgatgac acaggagttg atcgccaata tgctgggcgt gcgcagagaa
      181 ggcgtcacgc atgcggcggg gcgtttgcag gagaacgggc tgatctccta cgtgcgcggt
      241 cgaatcacga tcttggatcg tcgccgcctg gaagcgactg tttgcgagtg ttaccgtgtg
      301 gtcaaagacg agtacacccg tctactcggc tgattaaaaa cccctataat agcgattagc
      361 agaacccagc cagattcttc ctccgctcac ttgacgaccg cgcgattccc ttctatttgc
      421 tatacttact atgtcaataa actgtagttt cctgacaccc tatttctatc acctagaacc
      481 tccaaacagg ccgagatttt tactcccctt cctaaggagt tttttgacat ccctttaata
      541 cttacctcat aagatttgta atcaacgcta ccatcgtgca ccacgatcaa cagacacatg
      601 ctgtgccaac ttagagccat gcggtgtaca tctactagcg aaagtagaaa ctcgtaaaag
      661 aggctgaaga agactagaca actcttaacc ttttgaccct tttgtaaaga gtcgcagatg
      721 tagagtacag taaagacagg gcttccctgc cccgtctttc tatgcctcca acttccgtaa
      781 tgtaaagagt ttggatgata tggtgacgaa aagagtcaag aagcctgtcc cacaggccgt
      841 cgaccgcaag ggcaaggact tcctcacaga gggcgagatg aagcgtatgc tcgatgccgc
      901 gaagggcggc agatatgggg tgcgcgatta tctcctactg ttgatggcct atcgtcacgg
      961 cttgcgggtc tccgagttga ttgatctaag gcttaaagac cttgatctgg atacctcgcg
     1021 cctttacgtg cgccggaaga aaggcagcct ctcgacacac cagcccattg agggagatga
     1081 gctgcgcgcg ttgcgggcgt ggttgcgtgc gcggtcgctg cgagaggatg cgcgctcgcc
     1141 ttacctgttc ctgagcgaga gggggccctt tactcgtcag gcggtcaact acatcgtctc
     1201 tcaggccggt gggagggcgc gacttccctt ccgagtgcac ccgcatatgc tgcgtcactc
     1261 gaccggcttc tacctggcta acaagggatt cgatacgagg ctgattcagg actatctggg
     1321 gcaccggaac atcgcccata cggtgaagta cacgcgcacg gccgcgggga gatttgaggg
     1381 gctatggagg acgggcacag ggtgatgacc gggcggagca gagtgggtag aatgcaggag
     1441 acgagcggag tctccggtac agatataacc tgattagact gagcgacggg tcggtataac
     1501 accggcggaa tagacgtatt atacggaccg acgtatccgt ataatcacaa gttcggctgc
     1561 tgcaagttga atgataaagg gcgcgagact tgtcgctctg tgacggcgcg tggcttcttc
     1621 cctctccagt tgagttgtta tctttgacga ttcggacgca cgtgatcaaa gaagttctga
     1681 ttgactaata cagcggagga atgttcgtgt ccgcactcat taacctaatt gccaaggtga
     1741 aggctaaaga cctgcagctc ggcgccgact tggagcgcga gttccgggta ctctcatccc
     1801 ggttgtcctt tggcctgaat ttcgagcgtc atcggcccga agccgtggaa ttgccgcagc
     1861 gcccggtgcg ccggggcgac aaggtgcgcg tgctgccgcc gcgcggctcg accgccaggg
     1921 gcgaccagcg gctttggctg gtgaaggcga ttcatcgaga cacaaagacc gccgagctgg
     1981 aattgctgga cgcggcagat gccgagacgc agaccgtggc gctggacgat ttgatcgtcg
     2041 tcgccgaatt tcgcgacacc atctatccgg gactcgtctc cactggcagc gtctcgcgcg
     2101 gcggcgataa gccgtggcac acggtcatca atggcgagaa ctaccacgtg ttaaaagccc
     2161 tgacgtggac gcaccgcggc aaggtagatg cgatatacat cgacccgcct tacaacacgg
     2221 gggcgaggga ctggaaatac aacaaccact acgtcgaagg cgacgacctc taccgccaca
     2281 gcaagtggct ggcgatgatg gagcggcgat tgctggtcgc gaaagagttg ctgaagccag
     2341 atgactctgt tttgatcgtc acgatcgacg aaaaggagta tttgcgactg gggttactgt
     2401 tggagcaggt gtttcccgag gctcgacaga ctatggttag ctcgttgatc aacccaaagg
     2461 gttcggcgag atctgacact tttcgtcgct atgacgagta cttgtttttc gtcatgtttg
     2521 gcaaggcggc tccagtatct cttgaactat ctgacgagtg gcgtgtaact atcgatcgta
     2581 ggactcaaac gctgcaatgg gcttcattgc agcgaaaagg cgctggcggg tcaagatctt
     2641 cgtctccaaa tctgttttat ccggtgttca tccgaaacac tgctaacggt ccagtctttg
     2701 attctgtcgg cgagtcgttt tttggttcgg actgggacca agtcactgcc ccaagcggct
     2761 gtgttgcggt ctggccgata cgaagaaacg gttcggaagg ccgctggtcg acatcgccaa
     2821 gtacactacg tcgatatatc tcgcttggtt acgcgcagct gggccgctgg aacgaaggtg
     2881 gtacaactat caactatcta acaagcgggg agcagcgaaa gatcgaaacg ggggttttct
     2941 cgatcatcgc tcgccgtgcg gacaactctg ttctcattga cgccactaat tacaaaccgt
     3001 cttttgtgcc tggaactcaa tggcgaatca aagcgcacaa ctcaggtgat catggtgctt
     3061 cattggtggc tcagttgctt ccgaatcgtc gcttcccata tcccaaggct ttgtacgccg
     3121 tcgaagatgc tctgcgcttc ttcgtcgcca acaagcccga agctgtcatc ctcgatttct
     3181 tctccggctc cggtacgacc gcccatgccg tgatgcggct caacaagcag gacggcggca
     3241 ggcggcagtg tatcagtgtc actaacaatg aagttggcgc agacgagcag aaagcgttgc
     3301 gcgaaagagg tctgcgcccc ggcgatactg aatgggaatg tttgggcatc tgcgactaca
     3361 tcaccaagcc gcgtgtgcag gccgcgatta ccggcaagac gccggacggc gaaccgatca
     3421 agggcgacta caagttcacg gatgagttcc cgatgtcgga cggttttgag gagaacgccg
     3481 agttcttcac cctgacctac gaaacgccag tcgctgtcag ctatcagacc gcgttcgcgc
     3541 ggatcgcacc gctgctatgg ctgcgcgcgg ggagcgtggg tcgccgcatc gacaaactgc
     3601 ccgccgcggg ctgggatgtg gcagatgcct acgggctact cgtggaactg gacaaggcga
     3661 ctgacttcct caaagccgcg cgcgaggcga aggcgctgcg gatcgcttac atcgtcactg
     3721 acgacgagcg gcgctttcag gcactggcgc gacgcctacc cgaaggcgtc gaggcgatcc
     3781 gtctgtatga gtcctacctc accaacttcg ccttcgccaa cggagacaac gcatgaagtt
     3841 cacgctccac gattaccagg gcaaagccgt ccgcgacgtg ctgaacaatc tgaggaaagc
     3901 gcgcaaacgc tggcgcgagg acggcgaccg ccatgcattc tctctgaccg ccgtgaccgg
     3961 cgcgggcaag acggtgatgg cggcggctgt gttcgaggcg ctgtttcacg gcgacgacat
     4021 tctcgagttc gacgccgacc ctggcgcggt ggtgatctgg ttcagcgacg acccgtcgct
     4081 gaacgagcag acgcgctttc gcctgatgga gtcatccgac cggctccagc acaccgacct
     4141 ggtggtggtc gaaaacacct tcaaccgcgc gaagttcaac tccggaaaaa tctacttcct
     4201 caacacgcag aagctgggca agaaaagcct gctggtgcgc ggcttcgagg gcgacgagga
     4261 tgacagcgtg ttccccgaaa ttcggccgga catgcggagc cacacgatct gggacacgat
     4321 ccgcaacacg gttgaagacc cggcgctgac gctctaccta gtgctggacg aggcgcaccg
     4381 tggcatgggg agtgagaacc gcgagaagtc caccatagtc agccggctca tcaacggcga
     4441 aaaaggcgtg ccgggcatcc ccgtggtgtg gggcatttcg gcgacggtag accgcttcaa
     4501 caaggccatg gacggggcgc acaaccgcga cactctgcgg cccgtgaccg tggacaccgc
     4561 gctcgtgcag gagtctggct tgttgaagga caccatcgtg ctggacattc ccgacgaagc
     4621 gggcaacttc gacaccgtgc tggtgcgccg cgccacggac aagctgcgcg agtgcacgaa
     4681 ggagtgggcc gattacgccc ggcagcagcg aaccggcgaa accgtgctgc cgctgatggt
     4741 gttgcaggtg ccgaacacgc ccagtcacaa cgacatcggc cgcacgctgg acacgatctt
     4801 tcagcagtgg ccggagttgc aggagacaac ggtggcgcac gtgttcggcg aacacacgac
     4861 gcagacgttc ggcaaatact cggtgccata catccagccg cagcgcgtgc aggacgacac
     4921 atgggtgcgt gtcctgatcg ccaaggatgc aatca
//
LOCUS       DASWNS010000613         5324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_100887, whole genome shotgun sequence.
ACCESSION   DASWNS010000613 DASWNS010000000
VERSION     DASWNS010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100887"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..682
                     /locus_tag="VGB76_21020"
     CDS             152..682
                     /locus_tag="VGB76_21020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101420.1"
                     /translation="MTSKSLVTVVLLVSVVALTAKSGVAQAALDGGGKPARRTFTINY
                     GFTEEEEAAVTSKARECIWENWRRERPAHCAVRWTNIEAEPTTHNFYIDRGGDGRLQV
                     FLEIEYRCCWHYALEGKEPETKSMGTATYQIVERVDIKSNRVVPEKEDRAPHTYVLRF
                     KTGAPVKDEAATIRSL"
     gene            782..1588
                     /locus_tag="VGB76_21025"
     CDS             782..1588
                     /locus_tag="VGB76_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101421.1"
                     /translation="MMRSRSGFKSVAALALLSVLAWGAFEVQSQQQRRRPSRRATNPV
                     RTSSAPAPQATPDYEPRVISTAEEQAAQEGQTTNRARRNRPAPAATPDETLKTMQTLL
                     DEFGRLNKKVDDLEKERRVDLIQERLTRAEQRAEGLQTQLRDVLEKESNLQAQLDRVE
                     EQSRPDSIERQAALTGTFRPDELRDTLRRQYDAEKRRLRTQLDVLQGSRTRLESALGN
                     ADQLVQRLRSQLDDATQRELEADDSSSTAATPATPRATPAPTPSGTPPPQ"
     gene            1687..2073
                     /locus_tag="VGB76_21030"
     CDS             1687..2073
                     /locus_tag="VGB76_21030"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY0101422.1"
                     /translation="MSTISQAELYRLIAESNARGERVVVATVAHTRGSTPQRRGAKML
                     FFENGAVAGTVGGGCIEAEVWAEAREAMRTGACGLHKFSLTADEASEEGMVCGGTMEI
                     FIDVWDAAKKSGDELQAATADAVLTA"
     gene            2070..2354
                     /locus_tag="VGB76_21035"
     CDS             2070..2354
                     /locus_tag="VGB76_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101423.1"
                     /translation="MKGDKVALTLYETTCIGFLSGFAGGVLLYFAITLNAAAAAREMD
                     AMGSASHMCAAGSAAMWLAILSVPAGAIFGFCLGGFFLWRKAAAGRRPLP"
     gene            2459..2773
                     /locus_tag="VGB76_21040"
     CDS             2459..2773
                     /locus_tag="VGB76_21040"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEY0101424.1"
                     /translation="MPTSFMEDYKAKHQHPLNRLSHNIGIPLIVVSLPLFFFHWRWAI
                     ALFVAGWIFQFIGHAIEGNQPAFFRNPVYLLIGPLWLARRALSAVGLAKPTANSHARR
                     VR"
     gene            2776..3630
                     /locus_tag="VGB76_21045"
     CDS             2776..3630
                     /locus_tag="VGB76_21045"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY0101425.1"
                     /translation="MHEAGDHSNASARRIAEAIARILEDGEVAVLATLVEAPASPSSQ
                     HTTTTGAKLLFNSSGEQIAGGTRLPALEEELARRAVAFLDTRAEAQTFKVEEFAPQLA
                     EWRGARVLFERIENEPRIVVCGAGHVGASLARLAHATGYRVTLIDDRADFVTRARFPA
                     TEIELVAAVEGWERAVRAATGKGRGVFVAVVTRGHNEDEECLRAVVAARPDYVGMIGS
                     RRRTNIVLERLRETGVEAEILREVRAPVGLDIGAVSPEEVALAVLAEIVAKRRGGTGR
                     PLSEWRRE"
     gene            complement(3651..4490)
                     /locus_tag="VGB76_21050"
     CDS             complement(3651..4490)
                     /locus_tag="VGB76_21050"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY0101426.1"
                     /translation="MIREKLIEQEFGVSRKFRMRGREVSRVEALSDAVFGFAITLLVV
                     SLEVPRTFDALMEMMRGFIAFAICFAMLILVWYQQYKFFRRYGLNDALTFVLNAVLLF
                     VVLFYVYPLKFVWTLLVNLVLGVDTRVRLPTGEMVAPVAGSQMGQMMLVFSVGYVAVF
                     AVFTLLYWRAYTKRAELELNPLEVLDTRTELEENLLHIAVGLLSIGLASIGTRGYVAL
                     SGFCYMLIGPVMTVHAMLMGKKRKRLEARLMSSAADPEPDAEHEEEHPAKAAQIADVR
                     SQI"
     gene            complement(4516..5169)
                     /locus_tag="VGB76_21055"
     CDS             complement(4516..5169)
                     /locus_tag="VGB76_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101427.1"
                     /translation="MKSFTACLSSFVVSGCLLLFACHVLAASKPVRAQATPAAQPTAT
                     PQQQPAANMNNTNKPAPTPAANPSDVATLDAILAAVYDSISGAKGKARNWDRFRSLFI
                     PGARLIPTFKRPAGDFVSRVLTPEEFIASSGKFMEEQGFFEHGIANHVEQFANIAHAF
                     SVYEGRHDTADAKPFVRGINSIQLMNDGKRWWVVTIFWQAEDAANPLPQKYLDAKKK"
BASE COUNT         1055 a         1583 c         1744 g          942 t
ORIGIN      
        1 ggcgcgctca cgcattcggc gcgggcgatg gacatcagtt tcaagattca accgggctga
       61 gcgcgatttg cacgcgcgaa ggttggaact gcgccgttgc gtgagcgtgt aaacagagag
      121 ttcgagcgcg cccgttgccg ccagagtttt tatgaccagc aaaagtctcg tcacagtcgt
      181 gctgctcgta tcggtcgtcg ctttgacggc gaaaagcggc gtggcgcaag cggcgttgga
      241 tggcggcggt aagcctgcgc ggcgcacgtt cacgataaac tacggcttca cggaggaaga
      301 agaagcggcg gtaacatcga aggcgcgaga gtgcatctgg gaaaactggc ggcgggagcg
      361 tccggctcat tgcgccgtca ggtggacgaa cattgaagcc gagccgacga cgcacaactt
      421 ctatatcgac cgtggcggag acgggcgttt gcaggtgttc cttgaaattg agtaccgttg
      481 ttgctggcac tacgcgctgg aagggaaaga gccggagacg aaaagcatgg gcacggcgac
      541 ttaccaaatc gtggagcggg ttgatataaa gagcaaccgg gtggtgccgg agaaagaaga
      601 ccgcgcgccg cacacgtacg tgctcaggtt caaaacaggc gcgccggtca aggacgaggc
      661 ggcaaccatt cgttctttgt agcgtcggta gataaatctt tcgagacgat gggggcgagc
      721 gcgcagttcg acaaacgtta agcgtgtccc gcgttatccg tctaaagttg taaggagttt
      781 gatgatgcga agtcgttcag gtttcaagtc ggtggcggcg ttggccttgc tgtcggtgct
      841 ggcgtgggga gcgtttgaag tgcagtcgca acaacagcgc aggcgtccgt cgcggagggc
      901 gacgaatccg gtgcgcactt cgagcgcgcc cgcgcctcag gcaacgccgg actacgagcc
      961 gcgcgtgatc agcaccgccg aggaacaggc ggcgcaggag ggacagacga cgaaccgcgc
     1021 gcgccgcaat cgtcccgcgc ctgcggccac gcccgacgag acgctcaaga cgatgcagac
     1081 cttgctcgac gagttcggca gactcaacaa gaaggttgat gatctcgaaa aggagcggcg
     1141 cgtcgatctg attcaagagc gtctgacgcg cgccgagcag cgcgccgagg gattgcagac
     1201 gcagttgcgc gacgtgttgg agaaggagtc gaacttgcag gcgcaactgg atcgggttga
     1261 ggaacagtcg cgccccgaca gcatcgaacg gcaggccgcc ttgacgggca cgttccgccc
     1321 ggacgagcta cgcgacacgc tccgccgcca gtacgacgcg gagaagcggc gactccgcac
     1381 gcagctcgac gtgctacagg gaagccgcac gcgcctcgaa tccgcgctcg gcaacgccga
     1441 ccagcttgtc cagcgtctcc gctcgcagtt ggacgatgcg acgcagcgcg aactcgaagc
     1501 cgacgacagc agcagcactg ccgccacgcc cgccacgcca cgcgccacgc ccgcgccgac
     1561 accttcaggc acgccgccgc cacagtgaag ggatgaagga tgagggatga gggatgaata
     1621 agaaaagcct tcaactgcgt gagtgctttc tcttattcat ccctcatcct tcatccctaa
     1681 ttttttatga gtacgatctc acaggctgaa ctttatcgtc tgatcgcgga gtcgaacgcg
     1741 aggggcgagc gcgtggtggt ggcgacggtc gcgcacacgc gcggttcgac gccgcagcgg
     1801 cgcggggcga agatgctctt cttcgagaac ggagcggtcg cgggcacggt cggcggcggc
     1861 tgcatcgagg cggaggtgtg ggcggaagcg cgcgaggcga tgcgcacggg cgcgtgcggc
     1921 ctgcacaagt tcagcctcac ggctgacgag gccagcgaag agggcatggt ctgcggcggc
     1981 acgatggaga ttttcataga cgtgtgggac gccgcgaaaa aatcgggcga cgaactgcaa
     2041 gccgcaaccg ccgacgccgt tctgacagca tgaaaggtga caaagttgcg ttgacgttgt
     2101 acgagacgac gtgcatcggc ttcttgagcg ggttcgcggg cggggtgttg ctatattttg
     2161 ccatcacgct gaacgcagcg gcagccgcgc gtgaaatgga cgcgatggga agcgcgtctc
     2221 acatgtgcgc cgcaggctct gcggccatgt ggctcgcaat tctgagtgtc ccggcgggcg
     2281 cgatcttcgg cttctgcctc ggcgggtttt tcctctggcg caaagccgcc gccgggcgtc
     2341 gcccgcttcc ttgagcgtcc ggctgtcgcg ccgcgtgcga caggcatcag tctcgcctct
     2401 acagttacat tttcgcccgc cttccgtata atgaccccgc gcagaggaga gagacactat
     2461 gcccacatct ttcatggaag actacaaggc gaagcaccag cacccgctca atcgcctgtc
     2521 gcataacatc ggcatcccgt taatcgtcgt aagtttgccg ctcttctttt tccactggcg
     2581 ttgggcgatt gcgctcttcg tcgccgggtg gatttttcaa ttcatcggcc acgccataga
     2641 gggcaaccaa ccggccttct ttcgcaatcc ggtctatctg ctcatcgggc ctctctggct
     2701 cgcgcgccgc gcgctctccg ccgtggggct ggcgaaacct accgcaaact cacacgccag
     2761 acgcgtgcgc tgaaaatgca cgaagcgggc gaccactcga acgcctctgc gcgacgaatc
     2821 gccgaagcga ttgcgcgcat actggaagac ggtgaggtcg ccgttctcgc tacgctcgtc
     2881 gaagctcccg cttctccgtc gtcgcagcac acgacgacga cgggcgcaaa acttctcttc
     2941 aacagttcgg gcgaacagat cgcgggcggc acacgcctgc ccgcgcttga agaggaactc
     3001 gcgcgccgcg ccgtcgcctt tctcgacacg cgcgccgagg cgcagacatt caaagttgaa
     3061 gagtttgcgc cgcaactcgc ggaatggcgc ggggcacgcg tcctgttcga acgcatcgag
     3121 aatgaaccgc gcatcgtcgt ctgcggcgca gggcacgtcg gcgcgtcgct cgcacgcctc
     3181 gcgcatgcca ccggttatcg cgtgacgctc atagacgacc gcgcagactt cgtgacgcgc
     3241 gcacgctttc ccgcgaccga gatcgaactc gtcgcagccg tcgaaggttg ggagcgcgcg
     3301 gtgcgagccg caacggggaa gggacgcggc gtgttcgtcg ccgtcgtcac gcgcgggcat
     3361 aacgaagacg aagaatgcct gcgcgcagtc gtcgcggcga ggccggatta cgtcgggatg
     3421 atcggaagcc gccgccgcac caacatcgtg ctcgaacgcc tgcgcgagac aggggtggaa
     3481 gcagagattt taagagaagt gcgcgcgccc gtcgggctgg acatcggcgc ggtttcgccg
     3541 gaagaggttg cgctcgccgt gctcgcggag atcgtggcga agcgtcgcgg cggcacgggc
     3601 aggccgcttt cggagtggcg gcgcgaatag ttgaaggcgc ggcgagttga tcagatttgc
     3661 gacctcacat cggcgatctg tgcggccttc gcggggtgtt cttcctcgtg ctctgcgtcg
     3721 ggttcggggt cggctgcgct cgacattaag cgcgcttcga gtctctttcg tttctttccc
     3781 atcaacatgg cgtggacggt catcacgggt ccgatgagca tgtagcagaa gcccgatagc
     3841 gcgacgtacc cgcgcgtgcc gatgctggcg aggccaatcg agagcagccc gaccgcgatg
     3901 tgcaggaggt tctcttccaa ctccgtgcgc gtgtccaaca cttcgagcgg gtttagctcc
     3961 aactcggcgc gcttggtgta agcgcgccag tagagcagcg tgaagaccgc gaagacggcc
     4021 acgtagccga cgctgaagac gagcatcatc tgccccatct gactcccggc cacgggcgcg
     4081 accatctcgc ccgtcggcag gcgcacgcgc gtgtcaactc ccaataccag attcacgagc
     4141 agcgtccaga cgaacttcaa cgggtagacg tagaacagca cgacgaagag cagcacggcg
     4201 tttaagacga aggtgagcgc gtcgttcaaa ccgtaacggc ggaagaattt gtattgctgg
     4261 taccagacca ggatcagcat cgcgaagcag atggcgaagg cgatgaaccc gcgcatcatc
     4321 tccatcaacg cgtcgaacgt gcgcggcact tcgagcgaga cgacgagcag cgtgatggca
     4381 aacccgaaga ccgcatcgct caaagcctcg acgcgcgaca cctcgcgccc gcgcatgcga
     4441 aacttgcggc tcacgccgaa ctcctgctcg atcagctttt cacgaatcat ccgtgtgccc
     4501 tcgtcggcgt cgaagctatt tcttcttcgc gtcgagatac ttttgcggga gcgggttggc
     4561 ggcgtcctcg gcctgccaga agatcgtcac gacccaccag cgtttgccgt cgttcatcag
     4621 ttgaatgctg ttgatgccgc gcacgaaagg cttcgcgtca gccgtgtcgt ggcgtccctc
     4681 gtagacgctg aaggcgtgtg cgatgttggc gaactgctcg acgtggttgg cgatgccgtg
     4741 ctcgaagaat ccctgctcct ccatgaactt gccggacgac gcgatgaact cttcgggcgt
     4801 caggacgcgt gagacgaaat ctcccgccgg acgtttgaag gtcgggatca ggcgcgcgcc
     4861 cggaatgaag agcgagcgga agcgatccca gttgcgcgcc ttgcccttcg cgccggagat
     4921 ggaatcgtag acggcggcga ggatggcatc gagcgtggct acatcagacg gattggccgc
     4981 aggcgtcggc gcgggcttat tcgtgttgtt catgttggcg gcgggttgct gttgcggcgt
     5041 ggcggtaggt tgcgccgccg gagtcgcctg cgcgcgaacc ggtttcgacg ccgccagaac
     5101 gtggcaggcg aacaggagca ggcaaccgct cacgacgaac gaactcaggc acgctgtaaa
     5161 acttttcatg gaaattctcc tgaagattgt agatgaaagc tccgaacaaa ggggcgacgg
     5221 ctcgcacgtg caagcgatta gaccacgact tgagcgtcag ccgcaaaaac ttctcatgcg
     5281 acaaacttct tattcgtccg gcgaggcgaa atagttgcgt gagt
//
LOCUS       DASWNS010000614         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_333463, whole genome shotgun sequence.
ACCESSION   DASWNS010000614 DASWNS010000000
VERSION     DASWNS010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333463"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VGB76_21060"
     CDS             complement(<1..549)
                     /locus_tag="VGB76_21060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0101428.1"
                     /translation="MIIETLTVTAFQQQARVLGCEATRRAICIDPGDDAELILETLER
                     HGLTLQAIALTHAHLDHVGGVAALKSLCPGAEIILHAADEPLYRQLPEQPAWIGIPRA
                     QWKLMGFAYDAPPAVDRYWTDGESYEVGTLTFQALHCPGHTPGHVVLFEARERKVFVG
                     DCLFAGSIGRTDLPGGSYEQLMD"
     gene            662..1735
                     /gene="amrS"
                     /locus_tag="VGB76_21065"
     CDS             662..1735
                     /gene="amrS"
                     /locus_tag="VGB76_21065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEY0101429.1"
                     /translation="MPLGKKSTSLAGVLDELTLEAAPELTEHLAGNDLRCYACGHRCL
                     IREGKRGICKVRFNEGGRLLAPANYVAALACDPTEKKPFFHALPGSDTLTFGMLGCDL
                     HCGYCQNWLTSQALRDDAAGTAPQVVSSDRLVALAKAYGASLVGSSYNEPLITAEWAL
                     EVFRKAKPEGFKTAFISNGNATPQVLDYLRPWTDCYKIDLKSMNDRNYRQLGGVVDNI
                     LETVRMVHERGFWSEIVTLIVPGFNDSADEIKRAADFIASVSPDIPWHVTAFHQDYRM
                     NENANTTAEQLIRACETGREAGLRFVYAGNLPGRVGRYENTYCPACDELLIERYGYII
                     KQMKVSRDGKCPACAATIPGVWA"
     gene            complement(1832..>2366)
                     /locus_tag="VGB76_21070"
     CDS             complement(1832..>2366)
                     /locus_tag="VGB76_21070"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY0101430.1"
                     /translation="AFPEVSEFLQAWFRRTAELPSLEDFSQIPATISSNVVMRMLRTA
                     FKYDGLVITDDMEMGAVVQTLSVAEASLRAIEAGSDMILICEQEANFKAARDLICRSV
                     AEGRIKVEVLDKANRRIDRVLDLAGDYEHFDKEEFEALSRRFVELKRALHAAENDEEY
                     APAYGTPEDLERRSSNF"
BASE COUNT          476 a          709 c          721 g          460 t
ORIGIN      
        1 atccatcaac tgctcgtagg agccacccgg caaatccgtc cgcccgatag agcctgcaaa
       61 caggcagtcg cccacaaaaa ctttgcgctc gcgcgcctcg aacaagacga cgtggccggg
      121 cgtgtgccca ggacaatgca acgcctgaaa cgtgagcgtg ccgacctcgt aactttcccc
      181 atccgtccag taacgatcca ctgcgggcgg cgcgtcgtaa gcaaaaccca tcaacttcca
      241 ctgtgcgcgc gggatgccga tccacgcggg ctgttccggt aactgcctgt agagcggctc
      301 gtccgccgcg tgcagaataa tctccgcgcc ggggcataaa ctcttcaacg ccgccacgcc
      361 gcccacgtga tcgaggtgcg cgtgcgtcaa ggcgatggcc tgcaaagtga ggccgtgccg
      421 ttcgagcgtc tctaagatca actccgcgtc gtcgcccgga tcgatgcaga tggcacgccg
      481 cgtcgcctcg caaccgagta cccgcgcctg ctgttgaaac gcggtgacgg tgagtgtttc
      541 gatgatcatc ccggtagcct tcgggacata acttgacggt gaaattgcat ctaaagaaag
      601 tataaactga ccctcaagaa tctactaaca ggccaatttt taagcagagg atttaagtct
      661 catgccattg ggtaagaaat caacttcgct cgccggagtg ttggacgaat tgacgttgga
      721 ggccgcgccg gagttgaccg agcatctcgc cgggaacgat ctgcgctgct acgcctgcgg
      781 ccatcgctgc ctgatcaggg agggcaagcg cggcatctgc aaagtgcgct tcaacgaggg
      841 gggacgcctg ctcgcgcccg ccaactacgt cgccgcgctc gcctgcgacc ccacggagaa
      901 gaagcccttc ttccacgcgc tgcccggctc cgacacgctc accttcggca tgctcggctg
      961 cgacctgcac tgcggctact gccagaactg gctgacgagt caagccctgc gcgacgacgc
     1021 ggcgggaacc gcgccgcaag tcgtctcgtc ggatcgtctc gtggcgttgg cgaaggctta
     1081 cggggcatcg ctcgtcgggt cgagttataa cgagcctttg atcacggcgg agtgggcgtt
     1141 ggaagttttc cgtaaggcga agccggaagg gttcaagacg gccttcatct cgaacggcaa
     1201 cgcgacgccg caggtcttgg attacctgcg cccgtggacc gactgctaca agatcgattt
     1261 gaagtctatg aacgaccgca actacaggca actcggcggc gtggtagaca acattttgga
     1321 gacggtgcga atggtgcacg agcgcggctt ctggtcggag atcgtgacgc tcatcgttcc
     1381 cggcttcaac gattcggcag acgagatcaa gcgcgcagcc gatttcatcg cgagcgtgtc
     1441 gcccgacatc ccctggcacg tcacggcgtt tcatcaggat taccgcatga acgagaacgc
     1501 gaacacgacc gccgagcaac tcatacgcgc ctgcgagacg ggacgcgagg cgggcttgcg
     1561 tttcgtctat gccggaaacc tgccgggacg cgtcgggcgt tacgagaaca cctattgccc
     1621 cgcgtgcgac gaacttttga tcgaacgcta cggctacatc atcaagcaga tgaaagtgtc
     1681 gcgcgacggg aagtgccccg cgtgcgcggc gacgattccg ggcgtctggg cttagggaag
     1741 aagtgatgag tgatgagtga tgagtgatga gtgaaagaca agtctttggt tctcactcat
     1801 cactcatcac ttgcctttct cgtcactgct tttaaaaatt tgaggagcgg cgttcgaggt
     1861 cttcgggcgt gccgtaggcg ggcgcgtact cttcgtcgtt ttcggcggcg tggagtgcgc
     1921 gtttgagttc gacgaaccgg cgcgagaggg cttcaaactc ttccttgtcg aagtgctcgt
     1981 agtcgcccgc gaggtcgagc acgcggtcaa tgcggcggtt cgccttgtcg agcacttcca
     2041 ccttgatgcg tccttccgcg accgaacggc agatgagatc gcgcgcggcc ttgaagttcg
     2101 cttcctgttc gcagatgagg atcatgtcgg aacccgcttc gatggcgcgc agggaggctt
     2161 cggccaccga gagcgtctgc acgaccgcgc ccatctccat gtcgtcggtg ataacgaggc
     2221 cgtcgtattt gaaggccgtg cgcagcatcc gcatgacgac gttgctgctg atcgtcgccg
     2281 ggatttgact gaaatcttcg agcgacggca gttcggcggt gcgtcgaaac cacgcctgca
     2341 aaaactctga cacctcagga aaggcc
//
LOCUS       DASWNS010000615        25317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_385884, whole genome shotgun sequence.
ACCESSION   DASWNS010000615 DASWNS010000000
VERSION     DASWNS010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 25317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25317
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385884"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VGB76_21075"
     CDS             <1..707
                     /locus_tag="VGB76_21075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101431.1"
                     /translation="VFTVALAVGGSMALGGGATWLGGILGGILFLLLSAGALFLFFFI
                     VGRAAYVPQVMLVEGHGVFEAISRSIKLARGNVRRLTAMFLFTTFATYSALMLFIVPL
                     GWYGYLQGINPVSLTQTEWPVWYAVGYQVLTQASSILLAPVWMLGLSLLYVDERVRHE
                     GYDIELMAAQHLGEMPLLPGGAHAPLAPAISNSPTQPDSARHTPPPTPPREPREESFT
                     PLSNITTLGLSERKPR"
     gene            704..1822
                     /locus_tag="VGB76_21080"
     CDS             704..1822
                     /locus_tag="VGB76_21080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEY0101432.1"
                     /translation="MTRRRTFRRRTLRRRTPRANAGALAFCLALLCCYGAVAVRQADA
                     ASLEDYRARVLKATDAVEELYWAFDDTAEADSTEYRAHEAKTFADMRAALPATEKIEG
                     TGAAFDIDNRWLHNELDLYAKIAADKREDRNDALYRLMQRLRSLATQLSEAANAAAPA
                     GARNKEAEKGRLHSILRRPEYNQQAAEDTALARLWKKFKDAINKLMPESRPLNEGTAN
                     AFSLVARIFVFALTIAVIIFVVWKYGARLLPRNRPKKKESRGARVVLGERLAPGQTPA
                     DLLNEAESLARAGDIRGAIRKAYIAVLFELGERNILRLAAHKTNRDYLSAIRRERAAL
                     YGDMQPLTNNYERHWYGFAPATDADWHDFRTRCRQLTQ"
     gene            1905..3542
                     /locus_tag="VGB76_21085"
     CDS             1905..3542
                     /locus_tag="VGB76_21085"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY0101433.1"
                     /translation="MRGRFSIVVFVGVLLVALNAASYVRVEREAELELAPDRSTLNGN
                     PTGTRALYEFLDETGERVMRWREPPAALTRAPGTAAAPASARPATFVVVGALRVEFKE
                     EEVRSLMEWVEAGGRLVVIDRAPHAPLVESDEWVITPHIITPPGSDVRADDTEALTRN
                     VPPLAPAQPTLLTRDVERIAPSRFASRFVVAYKEHAAAANEGTGGIAPSPPGATSTPF
                     VIRQDPAAQNENAEVETTTQDEAASGGVGEADDETASSEESQPSLPPSSPVPPEIVPA
                     HTPGGTTVRDGEPSVVAETGESETAASDEASDAPQTSAPVFHLSDERGALLVDMARGR
                     GRIVVLSDPFIVANRGINRADNLQLAVNIVKGAGGGLIAFDEYHQGRGATRNQFAAYF
                     AGTPVLAICAQLFLLVAAVVWTRGRRFARPLPAPRVDRRSKLEFVASMAELQQRARAY
                     DLAIENVYGRTRRALARYAGADVSAPRATVAAGVAARSGRSREEIESLMRECEEAING
                     APVTARQSLALVARLRELERALGIRMRAREIRQARAR"
     gene            3878..4831
                     /locus_tag="VGB76_21090"
     CDS             3878..4831
                     /locus_tag="VGB76_21090"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0101434.1"
                     /translation="MLQNVSSTVEHILTELRKVIVGQDEVIEQVLVALLAEGHALIEG
                     VPGTAKTLLVKTLARIIGADFGRIQFTPDLMPSDITGTNVFNVATSSFSLRRGAIFTD
                     ILLADEINRTPPKTQAALLEAMEERQVTIDGELHPLSPLFTVLATENPIEYEGTYPLP
                     EAQLDRFLLKVIIGYPSAADEAQVVANWDRGFNARRLDQIDIHPLAQPDAITQCRAEV
                     RRTQMETGVQNYLVQIVRRTREHPSIAYGASPRASVALLLCAKALAAMRGREFATPDD
                     VRDITRPVLRHRLRLRAEAELDGATADTVITDILNTVEVPR"
     gene            complement(4938..7526)
                     /locus_tag="VGB76_21095"
     CDS             complement(4938..7526)
                     /locus_tag="VGB76_21095"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY0101435.1"
                     /translation="MKRSLLLSLVVALFVFNINLNAQEKDRSIRTRTKSLQKLDGYMP
                     LYWDAENGKLLLEIERFNTEFLYQVSLPTGVGSNPIGLDRGQLGSTNVVFFERTGQKV
                     LLVRPNYRYRALTDDAQERRAVEESFARSVLWGFKVEATEDARVLVDATAFFMRDAHG
                     VIETLRRTNQGRFQLDESRSSFYLARTKNFPQNTEVETQLTFTGDEPGRLVRETVPTP
                     QAITVRQHHSFVALPGAGYTPRRLDPRVGVMGVEFYDYASPFTEPIEKRWINRHRLQK
                     KDPAAASSEPVKPIIYYVDPGAPEPIRSALVEGASWWNEAFEAAGFRNAFQVKLLPPE
                     ADPMDVRYNMISWVHRSTRGWSYGGSIEDPRTGEIIKGNVTLGSLRVRQDFMLGTGMI
                     PSYNAGSGLRRGDDQGDASGSLLCELGMISAGDDDYLAAQDAATDSAAMSLARIRQLS
                     AHEVGHTLGLAHNFAASTYAGRASVMDYPAPLVEIRNGKLDLSNAYGRGVGAYDKFAI
                     NYAYAQFPPGADESVQLERIVEDGMARGMLFISDADARPLGAAHPLANLWDNGDDPVA
                     MLKHEMEVRRIGLAGFGLGNIPDGTPLSMLEAKLLPLYLHHRYQLQAAVKSVGGLYYT
                     YAVKRRAVSTVATDRMNGTSPARVQEIVAPARQREALHAVLDTLRPETLAIPPRILAL
                     IPPRAYGYEGGTGEYFANRADPVFDPIAAATIAADLAILPLLEPHRAARLNGFHALNP
                     ANPDFKQIIDALVARTWLDPAPRDTYHAGIARAVQSLTVTRLMDTAANADAAPQVRAA
                     ATEALRDLHERLKLPVADTTGGAHRRATRDDIERFLARPDAPRKQTVPLPIPQGDPIG
                     GGSRQP"
     gene            7749..9071
                     /locus_tag="VGB76_21100"
     CDS             7749..9071
                     /locus_tag="VGB76_21100"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0101436.1"
                     /translation="MRFVFSKLFYVLLALGFVPLSLAWSWPDLRWYTACYDATLLLLV
                     FIDARLSRQLPEGVSVSRELAGRFHIGAETEVRVQVANHTPREIGLRIKDEYPPELQL
                     STPREASLTLAAHTTATLVYGLTPPRRGRYAFGQIAVRYLSRLRLVWCTAQAGETEWI
                     KVYPNLRRAREAELKALGARSLVAAHRRSSWRGEGRDFESLREYVPGDELRHISWSAT
                     ARRGKLTTRQYQIERDQTILIALDAGRLMTARIEDESKFDSAIHAALALMSAAARGGD
                     NAGIAVFGRRVGAYIPPARGHEQMDAVLEALYGIEPEMIEPSYTRAFEYIAANCKRRA
                     LVVVLTDLVDEEGSRELLTSLKLLRPRHLPLVATIGDRDLSAAVRDTPESVRDLFTQS
                     VAEEIMFQREAALRMVERLGGLALDVTAATLAPNLLEAYIRVKERGLL"
     gene            9125..10987
                     /locus_tag="VGB76_21105"
     CDS             9125..10987
                     /locus_tag="VGB76_21105"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0101437.1"
                     /translation="MNAAISAIYERRHSRQLRASSLTLAAWALLFLITASPVEAQRPA
                     LRIDYTVKVESVKEQLFHVTADVKNISQPRLDLSLPTWTPGWYTVENYGKNILRFQIT
                     DARGRRLPHAMTRKQTWRVPTAGLNRIKVEFDYHANVLGLNQAKITNEWAFFTGIQLF
                     LLAEGHRASPASVRFDVPDNWRIISALKETRDAKVFTAPDYDTLVDAPTEMGNFDVIP
                     FDVEGKPHLFVATPAGVFPKDQNARLADMLTRIALAQRSIFGDLPYEKYVYFYFFAPP
                     ESNAGGGLEHLNSHVVVGAGGQNVKAEFLTGIASHEFFHLWNVKRLRPAEMWPYDYSR
                     ENESPLLWFSEGFTNYYGVLTENRAGTITRERFLQSVGDAAGGVESNEARKYISPADS
                     STSTWLGYDTPKAFDISYYTQGQNLGALLDLSIRQDTAGTRALDDVMRTLYQEFYKRG
                     RGFTTEDVIAVVTRLTRRDYRDFFRRYVAGVEVPPYDRIFGYAGYRLQKLTEQVPRLG
                     VGLNPVTEGVQITSVVPNSAAAQAGLIAGDVLMTIDGTVLKPDETGNVSLRELLARKL
                     NQTVKLGIRRGTEERTLEAKVATREETNYRLVELPRPTATQLKIREGWLRRAGN"
     gene            complement(11000..11971)
                     /locus_tag="VGB76_21110"
     CDS             complement(11000..11971)
                     /locus_tag="VGB76_21110"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEY0101438.1"
                     /translation="MSSDRFIKERKNAWQRLEDLLTLLDRMSLRRLHREEVRELGRIY
                     RRTSSDLAIARAESRDPRLVNYLNSLVIRAHGRIYRAEALEGGQRVRKFFLRDFPQTF
                     RRTWRYTGVAFACFMLFALAGFFGAWRDAEFSELAGISAAWREQNTEARVRWWETLNE
                     ANQVGGAAIMTNNIQVMFYAFAFGALAGGGTLYIMAMNGASIGAVLALTYRAGFGHEL
                     VAFMAGHGVIELTCIFIAGGAGLLVGGAILMPGDLSRFDSLRLRGREAIQLIVGCIPL
                     LVLAGIIEGFISPAPISPNIKFSIAILTGLALYTYLLLAGREIEQRD"
     gene            complement(12253..12897)
                     /locus_tag="VGB76_21115"
     CDS             complement(12253..12897)
                     /locus_tag="VGB76_21115"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEY0101439.1"
                     /translation="MLLEMNPISSLMAGWALMLVAMMSPTLITPLRHILNRSFKRRRA
                     RSVTLFVVGYAAIWMAASGVLIAVMLMLNLLAPQSYLPAVGVGIIAFVWQCSPIKQRC
                     LNRGHNHSELAAFGIAADLDALRFGITHGVWCVGSCWALMLLPMLLLQGHFAAMAVVT
                     FLMTGERLEQPRPLSWRLRFPGKLMRIAVAQTRIRLQSLPSGIRPSLAVLRKLF"
     gene            complement(13050..14594)
                     /locus_tag="VGB76_21120"
     CDS             complement(13050..14594)
                     /locus_tag="VGB76_21120"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEY0101440.1"
                     /translation="MVSFTRRNAWNNGGTFNNSDLLWYAAGVGVMQSRALDDPNSWWF
                     FAAIHGEYITGETAFPGWGFIPAPPQVPTTPLPSQNLQDMYWDQCQHQSWYFAPWHRG
                     YLLALEAQIRAAVVSLGGPSDWALPYWNYFGPVNEYEIPPAFTQLTLPDGVTPNPLYV
                     EARFGPNNDFNIFVPIPPVSETCEGNTIYTGSNAATPLPGFGGPTTGFWHGGENPSGN
                     LESNPHNQVHRYVGGNSPNNGPIYGLMADPGIAGLDPIFYLHHSNIDRMWAAWNANGN
                     SNPTDSNWLNGPTAVGDREFAMPFPDGTSWVYTPNDVNSLAQLDYIYDDLTVTVSPQP
                     SNKLAQRLRKLGAAPVTAKAAQGETMDSGGSAELVGAHDGALRVKTSGARATVRLDSD
                     VRGKVSASLAESSETNLPDHVYLKLENVRGTRDSYMLNVSVNQQRAGSVALFGLRKAT
                     LKDGQHGGGGLTFVLDITDIIDNLFLNSSLDADSLDVRIVPDDAIPDSEEITVGRVSV
                     YRQGQQ"
     gene            complement(14974..17496)
                     /locus_tag="VGB76_21125"
     CDS             complement(14974..17496)
                     /locus_tag="VGB76_21125"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0101441.1"
                     /translation="MKSSFEQKNINLYAKVILFVDALIFLAAIYNLPTAYLGLPLLLF
                     ALCAVHTCAAFPVKNTILGNHLYLTDSFLLLSLLLFDAEAAVVLAFVVTPCVLLHSSK
                     SVGLLIYRSAISVLPIFLAAPLLRFGFGSLPQLLEAQSVPAILTALAALVVFQSVLSA
                     AVSLHGRSASPQQGRKRTLAKSYLNSLCVYLMAGGVAGILAAVTHFIGLNTALLAGVF
                     AALAYFAYASFRNNHYISVATTGTTAVANNSSSNLNSASDEDRFRNAFDFAAIGMALV
                     SSEGRWLQVNRALCKILGYSEREMLVIDFLTVIHPDDLGVAVDAIKRLRKGNSTIDQC
                     EVRFRHKSGHKVWGLWSASLAGGPHVASTPLVFQIQDITDRKRAEEQLQHEAMHDVLT
                     GLPNRAMFIDHLELAIARAQRNEERKFAILYVDLDRFKIINDSLGHSVGDQLLKEVAL
                     RLWNCVRAGDTVARLGGDEFILLLEDVYAESEAIEVAERIKSELAAPFTLSGREVFTT
                     VSIGVASSWTSYHQAEGLIRDADAAMYRAKSLGKNRHEIYDSVMHAQVNDLLQMETAL
                     RLAVERREFIVYYQSIVDLETFKISGFEALVRWNHPEKGFISPAHFIPLAEETGLIVE
                     IGEWVLREACQQMERWQKIFPSDPPLFVSVNLSSKQFIQSDLIQRITQIITETKINPE
                     GLKLEITESAVMDDVDSATEMLKKLRALGIKLSIDDFGTGYSSLSYLHRFPIDTLKVD
                     RSFVVNMSEDSENVEIVRTIVSLAQNLGMNVIAEGVETKEQLAALRKLGCENGQGYLF
                     SKPVAAKAAENLICDTYTVEPSVESLVNSVGSQEKVVRYLVA"
     gene            complement(17714..17839)
                     /locus_tag="VGB76_21130"
     CDS             complement(17714..17839)
                     /locus_tag="VGB76_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101442.1"
                     /translation="MTATAAAERAVERRRAAFRPVERDSFVALSPRMAERDSTVM"
     gene            18023..18874
                     /locus_tag="VGB76_21135"
     CDS             18023..18874
                     /locus_tag="VGB76_21135"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY0101443.1"
                     /translation="MRWRGRRESTNVEDRRGVGRGGLAVGGGLGTIVLIVLALLFGAD
                     PRELLDQSGGQNPAQGTQSGRPANSQEDELKQFSSVVLAETEDVWQQLFQQAGRRYRE
                     PTLSLFTGQTRSACGAAGASTGPFYCSGDEKVYIDLSFYRDLRTRLGAPGDFAQAYVI
                     AHEVGHHIQHLLGTMEQVDAQQRRMSEASANRLSVMLELQADFYAGVWAHYAERRGII
                     EPGDIEEALNAATAIGDDRLQQRGQGYVVPDSFTHGTSEQRVRWFRKGYETGDMRQGD
                     TFSARNL"
     gene            18883..19398
                     /locus_tag="VGB76_21140"
     CDS             18883..19398
                     /locus_tag="VGB76_21140"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0101444.1"
                     /translation="MQLEIREARTKDELERLFPLLLLAEPSRSALRWSLRNLSDTAYR
                     MDVDGELAGAATMRWRRDPCEMVELAVAPERQGEGLGKKFVAWLVAEARRRGKAEIIV
                     GTSSTSAANLIFYQKCGFRVDHVRQDYFWYYDEPVYENGLPVRDMLVFRQDLAGAFAG
                     ETTEQAATDDS"
     gene            19438..21114
                     /gene="ftcD"
                     /locus_tag="VGB76_21145"
     CDS             19438..21114
                     /gene="ftcD"
                     /locus_tag="VGB76_21145"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEY0101445.1"
                     /translation="MERIVECVPNFSEGRRPEVIEQLIAAVESVDGTAVLDTHVDADH
                     NRSVITFVGGADTIVEAAVRVAARAAELIDLRQHVGVHPRVGALDVLPFVPVRGVTLE
                     DCIHLAHAAGARIARETGVPVYFYESAARKPDRVNLEDVRRGGFEQLREEIGVNPERA
                     PDEGAPRVHETAGACVVGARSLLIAYNINLHTTEISVARRIARAVRGRDGGLRFLKAL
                     GFELRERGLTQVSMNLVRFEKSALHHAFEAVRREAERYGVRVAGSEIVGLIPQAALDR
                     SAEYFLQIENFSPELILENRIASALARRREAARLERRRDDALAVSWSADVEAEAVARR
                     GVMPEHEVKRTANERSPRAPDASTHDAATTVPEEITGGGAAAAHAASLAAALGEMVAH
                     LVERKGKQEDMEREARDALQALSDLRARLAEAADADGASFAGVLGARRMPGRNDEERR
                     ARENALEEALKGAAAVPLETASLAVQVGELLETLAELGEPAWLSDAATGAQLALASTV
                     AARYNVLVNTSELEDEEFASEHVSRADDLLERAREIAARVEVMLLESIGR"
     gene            21136..21438
                     /locus_tag="VGB76_21150"
     CDS             21136..21438
                     /locus_tag="VGB76_21150"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0101446.1"
                     /translation="MENERREDARARVSIEAWWEGLSGRHQARVSDLSLGGCFIDSLG
                     KAEIGELIVFAIKQPDGGWLQLRGQVASVDQHVGFSLAFTYLTEDEQHALSELITP"
     gene            complement(21577..22605)
                     /locus_tag="VGB76_21155"
     CDS             complement(21577..22605)
                     /locus_tag="VGB76_21155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101447.1"
                     /translation="MLKRRILIKERHFATRDTNRIVRPFEWGTSFIAPHANGGDPRLI
                     LSAYAAEAVARSEEFYALPPINDYRLDGQRLTWTSAIETPAPENNTAHARFFRESTIT
                     GKENDTRRAVVILPHWNAQPDSYADLCHVFTRFGIAALRLSLPYHDARRTPELERADH
                     LVSSNIGRTLQSMRQAVLDTRAAVRWLHEQGYERIGIMGTSIGSCVGFLSLVHEPLVC
                     TGVFNHVSGYVADVAWRGISTAHVRAGFADSVTLDELRHYWLPISPLAYSEKLKHLRP
                     RPIRFIAARYDLTFPADLSRDAIEGVRKAGHPLDAVWLRCGHYTLGQSPWKYIDGWKI
                     VTFLRKHL"
     gene            complement(22812..24410)
                     /locus_tag="VGB76_21160"
     CDS             complement(22812..24410)
                     /locus_tag="VGB76_21160"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY0101448.1"
                     /translation="MKKPIFVIAISLAFVFALTIPRRDASASADKSADKAAAKLARSQ
                     EIAHDVIAARAEKHGIRDAKRELKATRVSKDDQEKTHTRFQQTYQDIPVFGGEAIVHL
                     NPDDSVFAVTDDLIDSVQADTTPHRTPDEAVRTAVSDLGCEDCLTAPPAADMQILRHE
                     GNDYLTYRVELRREDGTEQTSMPVYFVDAHTGNVVWNYDNLQTASGSSLYSGTVTINT
                     YFRSPSYYMEDLTRKIGTFDMRNTTSSVYRFTDADNVWNTSTQRAGVDAHYGAGKTYD
                     YFYNTHGRRGIDGAGGPVYYTSADGVTGLISSVVHYGVGYNNAFWNGSYMTYGDGNGS
                     SFSPLVTLDICGHEMTHGITERTARLTYSGESGALNESMSDVFGAMVERYTQGQTSNT
                     WKIGEQCYTPGVAGDALRYMDNPHSASNSGFTADDDPDHYAERYTGTGDSGGVHINSG
                     IGNKAFYLVAVGGTHHRGGSMTGIGADAAARIWYRALTSYMTSSTNFRGARTATLNAA
                     AALYGSGSTNYNAVAQAWSLCGVV"
     gene            complement(24891..>25317)
                     /locus_tag="VGB76_21165"
     CDS             complement(24891..>25317)
                     /locus_tag="VGB76_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101449.1"
                     /translation="SVSSYVSAAGGARVAQQQQQPSRGRVMPSAELKCDRSDLTLYDG
                     RVLAYRRRRGSIFLRIRTNFDTTEEVTIRHPATDDPSEFYLINGKAFTRSDWRRIERR
                     SKVLKSGMRANVWVCRDNPAIQPVVDWQPDDTGAYPRSR"
BASE COUNT         4973 a         7495 c         8093 g         4756 t
ORIGIN      
        1 gggtctttac ggtggcgttg gcggtgggcg gctcgatggc gctcggcggc ggcgcaacgt
       61 ggcttggcgg catcctcggc ggcatcctct ttctgctgtt gagcgcgggc gcgctctttc
      121 tcttcttctt catcgtcggg cgcgcggcct acgtgccgca ggtgatgctc gtcgaaggac
      181 acggcgtgtt cgaggccatc agtcgcagca tcaagctggc gcgcggaaac gtgcggcgtc
      241 tgacggcgat gtttctcttc accactttcg ccacctactc ggccttgatg ctcttcatcg
      301 tgccgctcgg ctggtacggc tacttgcagg gcatcaatcc ggtttcgctc acgcagacgg
      361 agtggccggt ctggtatgcc gtcggttatc aggtgttgac gcaggcgagt tcgatcctgc
      421 tcgcgcccgt gtggatgctc ggactctctt tgctctacgt ggacgagcgc gtgcgacacg
      481 aaggctacga catcgagttg atggccgcgc aacatctggg cgagatgccg ctcctgcccg
      541 gaggcgcgca cgcgccgctc gcgcccgcca tctccaactc tcccacacag cccgactccg
      601 cgcgccacac gccgccgcct acgccgccgc gcgagccgcg cgaagagtcg ttcaccccgc
      661 tctcgaacat caccacgctc ggactctcgg agagaaaacc gcgatgaccc gccgccgcac
      721 gtttcgacgc cgcacgcttc gacggcgcac gccgcgcgcg aacgccggag cgttggcgtt
      781 ctgcctcgct ctgctctgct gctacggcgc ggtcgctgtc cggcaggccg acgccgcttc
      841 gctcgaagac tatcgcgcgc gcgtcttgaa ggcgacggat gcggtcgagg agttatactg
      901 ggcgtttgac gacacggctg aggccgactc gacggagtac cgcgcacacg aagcgaaaac
      961 tttcgcggac atgcgcgctg cgctccccgc gacggaaaag atcgaaggaa cgggcgcggc
     1021 cttcgacatt gacaaccgct ggctgcacaa cgaactagac ctctacgcga agatcgccgc
     1081 cgacaaacga gaagatcgta acgacgcgct ctatcgtctc atgcagcgtc tgcgctcgct
     1141 tgccacgcaa ctctcggaag ccgcgaacgc cgccgcgcca gcaggggcgc gcaacaaaga
     1201 ggccgagaag ggtcgcctgc attccatcct gcgccgcccc gaatacaacc agcaggccgc
     1261 cgaagacacc gcgctcgcgc gcctctggaa aaaattcaag gatgcgataa acaagctgat
     1321 gccggaatcg cgcccgctca atgagggcac ggccaacgcg ttctcgctcg tcgcgcgcat
     1381 cttcgtcttt gccctgacca tcgccgtcat catcttcgtc gtctggaaat acggcgcgcg
     1441 actgttgccg cgcaaccgcc cgaagaagaa ggagtctcgc ggggcgcgcg tcgtgctcgg
     1501 cgaacgcctc gcgccggggc agacgcccgc cgatctcctg aacgaggccg aatcgctcgc
     1561 ccgcgcgggc gacatacgcg gcgcgatccg caaagcctac atcgccgtcc tgttcgaact
     1621 cggcgaacgc aacatcctgc gcctcgccgc gcacaagacc aatcgcgatt acctgtcggc
     1681 catccggcga gagcgcgccg cgctctacgg cgacatgcag ccgctcacga acaactacga
     1741 acgccactgg tacggcttcg cccccgcgac cgatgccgac tggcacgact ttcgcacccg
     1801 ttgccggcaa ctcacacagt agtgatgagt aatgagtgat gagtaatgag tgaaaaacaa
     1861 aagctcttac tcatcactca tcactcgtca ctcatcactt aaaaatgcgt ggaagattct
     1921 ccatcgtagt tttcgtcggc gtgctgctcg tcgctttgaa cgcggcttcg tatgtgcgcg
     1981 tcgagcggga ggcggagttg gaactcgcgc cggatcgttc gacgttgaac gggaacccga
     2041 cgggcacgcg cgcgttgtat gaatttttag atgagacggg cgagcgcgtg atgcgctggc
     2101 gcgaaccgcc cgccgcgctc acgcgtgcgc ccggcacggc tgctgctcct gcttctgcgc
     2161 ggcctgcgac gttcgtggtc gtcggcgcgt tgcgcgtcga gttcaaggaa gaggaggtgc
     2221 ggagtttgat ggagtgggtt gaggcgggcg ggcggctcgt cgtcatcgac cgcgcgccgc
     2281 acgcgccgct cgtcgagagc gacgaatggg ttatcactcc gcacatcatc acgccgccgg
     2341 gaagcgacgt gcgcgcagac gacacggaag ccctgacgcg taacgtgccg ccgctcgcgc
     2401 ccgcgcagcc gacactgctc acgcgcgatg tcgagcgcat cgcgccgtcg cgcttcgcaa
     2461 gccgcttcgt cgtcgcgtac aaggagcatg cagcagcggc gaacgaggga accggaggca
     2521 tcgcgccgtc gccgccgggc gcgacgagta cgccgttcgt tatcaggcaa gaccctgccg
     2581 cgcagaatga gaacgcggag gttgaaacga cgacgcagga tgaagcggcg tcgggcggcg
     2641 tcggggaagc agatgacgaa acggcgtcga gcgaagagtc gcagccgtcg ttaccaccat
     2701 cgtcgccagt gccgccggag attgtccccg cgcatacgcc agggggaaca accgttcgtg
     2761 acggtgaacc gagtgtcgtc gcggagacgg gcgagagtga gacggcggcg agtgatgaag
     2821 cgagcgacgc gccgcagacg agtgcgcccg tctttcatct gagcgatgag cgcggcgcgt
     2881 tgctggtgga tatggcgcgc gggcgcgggc gcatcgtcgt cctgagcgat ccgttcatcg
     2941 tcgccaatcg cggcatcaac cgcgcagaca atctccaact cgccgtgaac atcgtgaagg
     3001 gtgcgggcgg cggcctcatc gccttcgacg agtatcatca ggggcgcggc gcgacgcgca
     3061 accagtttgc ggcctacttc gcgggcacgc ccgtcctcgc catctgcgca caactctttc
     3121 tgctcgtcgc cgccgtcgtc tggacgcgcg ggcgacgctt cgcgcgtccg ctgcccgcgc
     3181 cgcgcgttga ccggcgttcg aaactggagt ttgtcgcttc gatggccgaa ctgcaacaac
     3241 gcgcacgcgc ctacgacctt gccatcgaga acgtctacgg acgcacgcgc cgcgcgctcg
     3301 cgcgctacgc aggggcggac gtttccgcgc cgcgcgccac ggtagccgca ggcgtcgccg
     3361 cgcgttcggg tcgaagtaga gaagagatcg aatcgttgat gcgcgaatgt gaagaggcca
     3421 tcaacggcgc gcccgtcacc gcgcgccaga gcctcgcgct cgtcgcacgt ttgcgcgaac
     3481 tcgaacgcgc gctcggcata cggatgcgcg cacgcgagat caggcaggcc agagcgcgtt
     3541 gagacggact ttgcggcagc actgcccgcg cgcacttcga cgcgtccgtc tccattggcc
     3601 ggagggattt ttgagagtcg ccgcgtgtcg cttaaagatt gcccgcgcgc cgcgcttgga
     3661 gtaaaacctt tgcgcgcagg tcgaaaccgc cgcccgccgc gcaggccgcc gcgcgctgaa
     3721 cttcagattc accaaattca acggaaggaa aggaagtgat gagagtaagt gatgagtgat
     3781 gagtgatgcg tgatgagtga aagacaaggc tgttgtttta gctcatcact cattactcat
     3841 cactcatcac ttacttccgt cactcattat ttaaaagttg ctgcaaaacg tttcctcaac
     3901 ggtcgaacat atcctgacgg agttacgcaa agtgatcgtc gggcaggacg aggtgatcga
     3961 gcaggtgctc gtggcgttgc tggccgaagg gcacgcgctc atcgaaggcg tgccgggcac
     4021 ggcgaagacg ctgctggtca agacgctcgc gcgcatcatc ggcgcggatt tcggtcgcat
     4081 ccagttcacg cccgacctga tgccttcgga catcaccggc accaacgtct tcaacgtcgc
     4141 cacgtccagc ttctcgctcc ggcgcggcgc gatcttcacg gacatcctgc tcgcagacga
     4201 gatcaaccgc acgccgccga agacgcaggc cgcgctcctc gaagcgatgg aggaacggca
     4261 ggtgacgatt gatggcgaac tccatccgct ctcgccgctc ttcaccgtgc tcgccaccga
     4321 gaaccccatc gaatatgaag gcacgtaccc gttgcccgag gcgcagcttg atcgctttct
     4381 cttgaaagtg atcatcggct acccgtcggc tgcggacgaa gcgcaggtcg tcgccaactg
     4441 ggacaggggc ttcaacgcgc gccgcctcga tcaaatagac atccacccgc tcgcacagcc
     4501 ggacgccatc acgcaatgtc gcgccgaagt gcgacggacg cagatggaga cgggcgtgca
     4561 aaactatctc gtccagattg tccggcgcac gcgcgaacat ccatccatcg cttacggcgc
     4621 aagcccgcgc gcttcggtcg cgctcctgct gtgcgcgaaa gccctcgccg ccatgcgcgg
     4681 tcgggagttt gccacgcccg acgacgtgcg cgacatcacg cgcccggtgc ttcgccaccg
     4741 cctgcgcctg cgcgccgaag ccgaactcga cggcgcaacc gccgacacag tgatcacgga
     4801 tattctcaac acggtcgaag tgccgaggta agagttaaca ggtcgtcgcg caccatgttc
     4861 ataaatgaga agtccggcca actatggccg gacttttttg ttgcgcttgt ttgcgaggcg
     4921 aaacaacgtg cgtatcgtca aggctgacgg ctgccgcctc caatcgggtc gccctgcggg
     4981 atgggcaggg gcactgtctg tttgcgcggg gcgtcggggc gcgcgaggaa tcgttcgatg
     5041 tcgtcgcgcg tggcgcggcg gtgcgcgccg ccggtcgtgt cggcgacggg aagtttgagc
     5101 cgctcatgca ggtcgcgcag ggcttcggtg gcggcggcgc gcacttgcgg cgcagcgtcc
     5161 gcgtttgcgg cggtgtccat caagcgcgtc acggtgagac tctgcacggc gcgtgcgatg
     5221 ccagcgtgat aggtgtcgcg cggcgcgggg tcgagccagg tgcgcgcgac gagcgcatcg
     5281 atgatctgtt tgaagtcagg gttggcgggg ttgagcgcgt ggaacccgtt gagacgcgcg
     5341 gcgcggtgcg gttcgagcag cgggagaatg gcgaggtctg cggcgatggt ggcggcggca
     5401 atcggatcga agacggggtc ggcgcggttc gcgaagtatt cgccggtgcc gccttcgtag
     5461 ccgtaggcgc gcggcgggat gagtgcgagg atgcgcggcg ggatggcgag cgtctccggt
     5521 cggagcgtgt cgagcacggc gtgcaaggct tcgcgttggc gcgcgggcgc gacgatctcc
     5581 tgcacgcgcg ccggactcgt gccgttcatg cggtcggttg cgacagtgct gactgccctt
     5641 ctcttgacgg cgtaggtgta gtacagcccg ccgacggatt tcaccgccgc ttgaagttga
     5701 tagcgatgat gtagatagag cggcaggagt ttcgcttcga gcatcgagag cggcgtgccg
     5761 tcggggatgt tgccgaggcc gaaacctgcg aggccgatgc ggcgcacttc catctcgtgt
     5821 ttgagcatcg caaccggatc gtcgccgttg tcccagaggt ttgcgagcgg gtgcgccgcg
     5881 ccgagcggtc gcgcgtctgc gtcggagata aagagcatgc cgcgcgccat gccgtcttcg
     5941 acgatgcgtt cgagttgcac ggactcatcc gcgcccggcg gaaactgcgc ataggcatag
     6001 ttgatcgcaa acttgtcgta agccccgacg ccgcgcccgt aggcgttgga caaatccagc
     6061 ttgccgtttc tgatttcgac gagcggcgcg gggtagtcca tgacggacgc gcgacccgcg
     6121 taggtgctcg cagcgaagtt gtgcgcgagg ccgagcgtgt ggccgacttc gtgcgccgag
     6181 agttggcgga tgcgcgctaa agacatcgcg gcggaatcgg tcgcagcatc ctgcgccgcg
     6241 agatagtcgt cgtctccggc ggagatcatg ccgagttcgc aaagcagact cccgctcgcg
     6301 tcgccttgat catcgccgcg ccgcaggccg ctcccggcgt tgtaggaagg gatcatgcct
     6361 gtgccgagca tgaagtcctg gcgcacgcgc aaacttccga gcgtgacatt gcctttgatg
     6421 atctcgcccg tgcgcgggtc ttcgatgctc ccgccgtaag accaaccgcg cgtcgaacga
     6481 tgcacccagc tgatcatgtt gtagcgcacg tccatcgggt cggcttcggg gggcaggagt
     6541 ttgacttgaa aagcgttcct gaacccggcg gcctcgaagg cttcattcca ccacgacgcg
     6601 ccttcgacga gcgcgctgcg aatgggttcg ggcgcgcccg gatcaacgta gtagataatc
     6661 ggcttgacgg gttctgaaga cgcggctgcc gggtctttct tctgcaagcg atgacgattg
     6721 atccaacgct tctcaatcgg ctcggtgaag ggcgacgcat aatcgtaaaa ctcgacgccc
     6781 atcacgccga cgcgcgggtc gagacgacgc ggcgtgtagc ctgcgcccgg cagcgcgacg
     6841 aacgaatgat gctggcgcac ggtgatggcc tgcggcgtgg gcacggtttc gcgcacgagc
     6901 ctgcccggtt cgtcgcccgt gaaggtgagt tgcgtttcga cttccgtgtt ctgcggaaaa
     6961 tttttggtcc gcgcgagata aaaactgctc cgcgattcgt ccaactgaaa gcggccttga
     7021 ttcgtgcggc gcagagtttc aatgacgccg tgcgcgtcgc gcatgaagaa ggcggttgcg
     7081 tccacgagca cgcgcgcatc ttccgtcgct tcgactttaa agccccacaa cacggagcgc
     7141 gcaaacgatt cttcgaccgc gcgccgctcc tgcgcatcgt ccgtgagggc gcgataacga
     7201 tagttcggcc tgacgagcaa gaccttctgc cccgtgcgct cgaagaagac gacgttggtg
     7261 ctgccgagtt gccctcgatc aagcccgatg ggatttgaac cgacgcccgt aggcagagac
     7321 acctgataca gaaactccgt gttgaagcgt tcaatctcca acagcagctt accgttttcg
     7381 gcgtcccagt agagcggcat gtagccgtcc agtttttgca gactcttcgt gcgcgtgcga
     7441 atgctgcggt ctttctcctg cgcgttcagg ttgatgttga agacgaagag agcgaccacg
     7501 agcgagagca gcagggaacg tttcatcgga tacctcgaat gatgttgtga ggcgcgacca
     7561 ccgccgcacg gatggataaa ctcggagaaa ctcggagcgc acattctagc cctgttcgtc
     7621 cgggtaggct agaacttttc gaaagccgtg cgggaaacaa gtttgccaac atggctggct
     7681 gccgttagta tgccggaaca aggtttgttc aagacgcttc acgcggggat cgcacccgca
     7741 cagacgtaat gcgcttcgtc ttttcaaaac tcttctacgt gctgctggcg ctcggcttcg
     7801 tgccgctctc gctcgcgtgg tcgtggcctg atctgcgctg gtacacggcc tgctacgatg
     7861 cgacgctgct cctgctcgtc ttcatagatg cgcgcctcag ccgtcagctt ccggagggcg
     7921 tctccgtctc gcgcgaactg gcggggcgtt ttcacatcgg cgcggagacg gaggtgcgcg
     7981 tgcaggtcgc caaccacacg ccgcgcgaga taggcctgcg catcaaggac gagtacccgc
     8041 cggaactgca actctcaacg ccgcgcgagg cgtctctgac gctcgcagcg cacacgacgg
     8101 ccacgctcgt ctatggactc acgccgccgc gacgcgggcg ttatgcgttc ggccagatcg
     8161 cggtgcgtta tctttcgcgc ctgcgtctgg tgtggtgcac ggcacaggcg ggcgagaccg
     8221 agtggatcaa ggtctatccc aatttgcggc gcgctcgtga agcggagttg aaggcgctcg
     8281 gagcgcgttc gctcgtcgcg gcgcacaggc gcagttcgtg gcgcggcgag gggcgcgatt
     8341 tcgagtcgct gcgcgaatac gtgccgggcg acgagttgcg ccacatcagt tggagcgcga
     8401 cggcgcggcg cggaaaactg accacgcgcc agtatcaaat cgaacgcgac cagacgatcc
     8461 tgatcgcttt ggacgccggg cgtttgatga cggcgcgcat cgaagacgag tcgaagttcg
     8521 actcggccat ccacgccgcg ctcgctctga tgagcgcagc cgcacgcggc ggcgacaatg
     8581 ccgggatcgc cgtcttcggt cgccgcgtcg gcgcatacat cccgcccgcg cgcggccacg
     8641 aacagatgga cgcggtgctc gaagctctct acggcatcga accggagatg atcgagccgt
     8701 cctacacgcg cgccttcgag tacatcgccg ccaactgcaa acggcgcgcg ctcgtcgtcg
     8761 tcctcacaga cctcgtggac gaagaaggct cgcgcgaact gctcacctct ttgaaacttt
     8821 tgcgcccgcg ccatctcccg ctcgtcgcca ccatcggcga ccgcgacctg agcgcagccg
     8881 tgcgcgatac gccggagagc gtccgcgatc tcttcaccca atcggtcgcc gaagagatca
     8941 tgttccagcg cgaagccgcg ctccgcatgg tcgaacgcct cggcggcctc gccctcgacg
     9001 tgaccgccgc caccctcgcg cccaacctgt tagaggctta catccgcgtt aaagagcgcg
     9061 ggttgttgtg aagcgggaaa ttttgagaac tcaggaatct cggaaatatc gagacgagaa
     9121 aaatatgaac gctgccatct ccgctatcta cgagcgacga cacagtagac aacttcgcgc
     9181 gtcttctctg acgctcgccg cgtgggcact cctcttcctg atcacggcgt cgcccgtcga
     9241 ggcgcaacgc ccggctctgc gcattgatta cacggttaaa gtcgagagcg tcaaagagca
     9301 actctttcat gtgacggcgg acgtgaagaa catcagtcag ccgcgtctcg atctatcgct
     9361 cccgacgtgg acgcccggtt ggtacaccgt cgaaaactac ggcaagaaca tcctccgctt
     9421 ccagatcacg gacgcgcgcg ggcgtcgtct gccgcatgcg atgacgcgca aacagacttg
     9481 gcgcgtgccg acggcgggac tcaaccgcat caaagtcgag tttgattatc acgcgaacgt
     9541 gctcgggctg aatcaggcga agatcacgaa cgagtgggcg ttcttcacgg gcatccagtt
     9601 gttcctgctg gccgaagggc atcgcgcgtc gcctgcgagc gtgcgcttcg acgtgccgga
     9661 caactggcgg atcatctcgg ctctcaaaga gacgcgcgat gcgaaagtct ttaccgcgcc
     9721 ggattacgac acgctcgtcg atgcgccgac cgagatgggc aacttcgacg tgattccctt
     9781 cgacgtggaa ggcaagccgc atcttttcgt cgccactccg gcgggcgtct ttcccaaaga
     9841 tcagaacgca cggctcgcag acatgctcac gcgcatcgcg ctcgcgcagc gcagcatctt
     9901 cggcgacttg ccctatgaga agtacgtcta tttctatttc ttcgcgccgc cggaatcgaa
     9961 tgccgggggc gggttggagc atctcaattc gcacgtcgtg gtcggagccg gagggcagaa
    10021 cgtcaaagcg gagtttctaa cgggcatcgc ctcacacgag ttcttccacc tctggaacgt
    10081 gaagcgcctg cgccccgccg agatgtggcc ttacgactac tcgcgcgaaa acgaatcgcc
    10141 gctcctgtgg ttctccgaag gcttcacgaa ctactacgga gtgctcacgg agaatcgcgc
    10201 gggcacgatc acgcgtgagc gttttctaca gtcggtcggc gatgcagccg gaggcgtcga
    10261 gtcgaacgaa gcgcgcaagt acatctcgcc cgcagactct tccacctcca cgtggctcgg
    10321 ctacgacacg ccgaaagcct tcgacatctc ctactacacg caaggccaga atctcggcgc
    10381 gctcctcgat ctctcgattc gtcaggacac ggcgggcacg cgcgctctcg acgacgtgat
    10441 gcgcacgctc tatcaggagt tctacaaacg cggtcgcggc ttcacgaccg aagacgtgat
    10501 cgccgtcgtc acacgcctca cgcgccgcga ctacagagac tttttccggc gttacgtcgc
    10561 gggcgtcgaa gtgccgccct acgacaggat tttcggctac gcgggctatc gcctccagaa
    10621 gttgaccgaa caggtgccgc gcctcggtgt cggcctcaac cccgtgacgg agggcgtgca
    10681 gatcacgagt gtcgtgccca actccgccgc cgcgcaggcc gggttaatcg cgggcgatgt
    10741 tttaatgacg attgacggaa cggtcttgaa accggacgag acgggcaacg tctctttgcg
    10801 cgaactgctc gccagaaagc tcaatcagac ggtcaagctc ggcatcaggc gcggcacgga
    10861 ggaacggacg ctcgaagcga aagtcgcgac gcgcgaggag acaaactatc gcctcgtcga
    10921 actgccgcgc ccgaccgcta cgcaactgaa aatccgcgaa ggctggctgc gtcgagccgg
    10981 gaattgatcg gcgagagtct taatcgcgct gctcgatctc tctgcccgca agcaatagat
    11041 aggtgtagag ggcgaggccg gtgagaatgg cgatgctgaa ttttatgttt ggcgagatcg
    11101 gtgcgggcga gatgaagcct tcgatgattc cggcgaggac gagaagcggg atgcagccga
    11161 cgatgagttg aatggcttcg cgcccgcgca ggcgcagcga gtcgaagcgt gagagatcgc
    11221 cgggcatcag gatcgcgccg ccgacgagca gccccgcgcc gcccgcgatg aagatgcagg
    11281 tcagttcgat gacgccgtgc cccgccatga aggcgacgag ttcgtggccg aaccctgcgc
    11341 gataggtgag cgcgagcacc gcgccgatgc tcgcgccgtt catcgccatg atgtagagcg
    11401 tgccgccgcc tgcgagcgcg ccgaaggcga aggcgtagaa catgacctgt atgttgttgg
    11461 tcatgatcgc cgcgccgcct acctgattcg cctcgttcag agtctcccac cagcgcacgc
    11521 gcgcctccgt gttctgttcg cgccacgccg cgctgatgcc cgcgagttcg gaaaactccg
    11581 catcgcgcca cgctccgaag aatccggcca gagcaaagag catgaagcag gcgaaggcca
    11641 cgcccgtgta acgccacgta cggcgaaacg tctgcggaaa gtcgcgcagg aaaaacttgc
    11701 gcacgcgctg cccgccttcc agagcctccg cgcgatagat acgaccgtgt gcgcggatga
    11761 cgagactgtt gagatagttg acgaggcgcg ggtcgcgcga ttcagcgcgt gcaatcgcca
    11821 gatcggagga ggtgcggcga tagatgcgcc cgagttcgcg cacctcttcg cggtgcaggc
    11881 ggcgcagaga catccggtcg agcagcgtca acaaatcttc cagccgctgc caggcgttct
    11941 tgcgttcttt gatgaagcga tccgaggaca taaaaaaaga tgccagctac cagatgccgg
    12001 atataagcat tatacgaatc agtagttgag aacttcctgt gtcgaaagca aatggccatg
    12061 tgcgagcgtc acaatgcttc aaaagatttc acacgacgga gcgaactatc gaaggcattg
    12121 aggcggtaaa catgataagg aaaggccggg tcaaaagtta gccggaagtg ataggcgagg
    12181 gcgggttaag ttcgtcgctt cgctctttca gctcgccgct caaggaagtt atagcgtgtg
    12241 accttctgca ctctaaaata attttcgcaa cactgccaga gatggtctaa tacctgacgg
    12301 caaactttgc aatcgtatcc gagtctgcgc gaccgcaatc cgcataagct tgccgggaaa
    12361 gcgcagtcgc caactcagcg gtcttggttg ttcaagacgt tcgccggtca tcaggaaggt
    12421 tacaacagcc atcgcggcaa aatgcccttg tagcagcagc atagggagta gcatcaacgc
    12481 ccaacaggag ccgacgcacc aaaccccgtg cgtaattccg aaacgcagag cgtccaggtc
    12541 tgcggcgatg ccaaacgcgg ccagttcact atgattatgg ccgcgattaa ggcaacgctg
    12601 ctttatgggc gaacactgcc acacgaaggc aataatcccc accccgaccg ccggcagata
    12661 tgattgcggc gcaagcaggt tcagcataag cattactgcg atcagcacgc cgctcgccgc
    12721 catccaaatc gcagcataac cgaccacaaa aagagtaacg gagcgagcac gccgacgctt
    12781 gaagctgcgg ttgaggatgt ggcggagcgg tgtaatcaac gtcggcgaca tcatcgcgac
    12841 cagcatcagc gcccagcccg ccatcagaga agagatcggg ttcatctcca gcagcatctg
    12901 aaatgaaacc agcgatgctc ccgagtttgt tacctgacag tgagcaagag tcatgatgct
    12961 cccggggtta accaggagca tcatccacgt taccgcgctt atgagcagta tcgagatatt
    13021 aacccgtgca ttctctcggg cacttattgt cattgctgcc cttggcggta aacgctgacg
    13081 cgcccaacgg tgatttcctc actatccgga atagcatcat cgggcacaat tctcacatcg
    13141 agcgaatccg catcgagaga gctgttgagg aataggttgt caatgatgtc cgtgatgtcc
    13201 aacacgaagg tgagcccccc gccaccatgt tgaccatcct ttaaggtagc cttccgaagt
    13261 ccgaaaagtg caacagagcc tgctctctgt tggttgacag agacgttgag catatatgag
    13321 tctctggttc ctcgaacatt ttcaagcttg aggtagacat gatccggcaa gttcgtttca
    13381 gaagattccg cgaggctcgc tgacaccttc ccccggacgt cagaatcaag cctaacagtt
    13441 gcgcgagccc ccgaggtctt tacccggaga gctccgtcgt gggctccgac gagttccgca
    13501 ctacctccgc tgtccatagt ttctccttgc gctgctttcg ccgtgacagg tgctgctccc
    13561 aactttctca acctttgggc aagtttgttg gagggttggg gtgaaacagt caccgtcaga
    13621 tcgtcataaa tataatccaa ctgcgcgaga ctgtttacat cgttcggggt atacacccag
    13681 gaagtgccgt ccggaaatgg cattgcgaat tcccgatcac cgacggctgt gggtccgtta
    13741 agccagttcg agtcggtcgg gttgctgttt ccgttcgcgt tccacgccgc ccacatccgg
    13801 tcaatgttgc tatggtgtaa atagaagatg ggatcgagtc ccgctatgcc cggatcagcc
    13861 atcaagccat agattgggcc attattcgga gagttgcccc caacgtaacg gtgcacctga
    13921 ttgtggggat tgctttcgag gttgccgctg gggttttctc caccatgcca gaaccctgtc
    13981 gtcggaccac cgaagccggg aagcggagtg gcggcgttgc tgccggtata gatagtattg
    14041 ccctcgcaag tctctgatac cggaggaatg ggaacaaaga tgttaaagtc gttattgggt
    14101 ccgaaccggg cttctacata aagggggttc ggggtcaccc cgtccggcaa agtcagctga
    14161 gtaaaagcag ggggaatctc atattcattg actggtccga aataattcca gtagggcaaa
    14221 gcccagtccg aaggaccacc gagactcacc accgccgcac ggatttgcgc ttccagtgct
    14281 aacagatacc cacgatgcca cggagcgaaa taccaacttt ggtgctgaca ctgatcccaa
    14341 tacatgtcct gaagattctg ggatggaagc ggtgtcgttg gcacctgcgg cggcgcaggg
    14401 ataaaacccc agccgggaaa tgcagtttcg ccggttatgt actcgccatg gatggcggcg
    14461 aagaaccacc aactgtttgg atcatctaaa gcgcgcgact gcattacgcc gacgcctgcc
    14521 gcataccata agaggtccga attgttgaat gtcccgccgt tattccacgc attccttctt
    14581 gtgaagctga ccattatctt acctccgctt agtgagtaat attccggagc tggattttgg
    14641 cgagttatgc aagaggcaga ggctcgtgag gattcagccc tttggattcg agcgcgggca
    14701 gcatactccg acacctgttc gatgtcaata aagtaggata tgcgaattca aaagaacaga
    14761 tttacagaac gctgcgcgcg ttcggtcgag tcaagggttg aaagtgcctt gctagggcaa
    14821 acgcgttgtt gcctgtctat tgctcacttt caactcatga ttcgcatagc attacatgat
    14881 tcgcatagca ttatctgata gctagcgtct ttaggttggc ggactttgga agggaaggat
    14941 ggcgggcgtc tcgctgtctc gtcgccgaca ctgtcaggcg acgagatagc gcacaacttt
    15001 ttcctgcgag ccgaccgagt tgacgagcga ctcgaccgag ggttcgaccg tgtaggtgtc
    15061 gcagatcaaa ttctcggcgg ctttcgcggc gacgggtttg gagaacaggt agccctgccc
    15121 attttcgcag cccagttttc ggagcgcggc gagttgctct ttcgtctcca cgccctctgc
    15181 gatgacgttc atgccgaggt tctgcgcgag actcacaatc gtgcggacga tctccacgtt
    15241 ctcgctatcc tccgacatgt tgacgacgaa ggagcgatca actttcaaag tgtcaatcgg
    15301 gaaacgatgc aggtaactga gcgacgaata gcccgtgccg aaatcgtcga tgctcaactt
    15361 gatgccgagc gcgcgcagct tcttgagcat ctcggtcgcg gagtcaacgt cgtccatcac
    15421 cgcgctctcg gtgatctcca gtttcaatcc ctccgggttg atcttcgtct ctgtgatgat
    15481 ttgcgtgatc cgctgaatga gatcgctctg gatgaactgc ttgctcgata agttcacgct
    15541 gacgaacaga ggcggatcag aggggaatat cttttgccag cgttccatct gctggcacgc
    15601 ctcgcgcagc acccattcgc cgatctccac gatcaagccc gtctcttcgg cgagcggaat
    15661 gaaatgggcg ggcgagatga aacccttctc cgggtgattc cagcgaacga gggcttcgaa
    15721 gccggagatt ttgaaagtct ccagatcaac gatggactgg tagtagacga tgaactcccg
    15781 acgctcgacc gcgaggcgca aggccgtctc catctgcaac aggtcgttga cctgcgcgtg
    15841 catcacggag tcgtaaattt cgtgacggtt tttaccgagc gacttcgcgc ggtacatggc
    15901 cgcgtccgcg tctctgatca atccttcggc ctgatgatag ctggtccaac tcgaagccac
    15961 gccgatgctc accgtcgtaa acacctcgcg cccgctcagt gtgaagggag cggccagttc
    16021 gctcttgatg cgttcggcca cctcaatcgc ttcgctctcc gcatagacat cttcgagcag
    16081 gaggataaac tcgtcgccgc cgagccgcgc caccgtgtcg cccgctctca cgcagttcca
    16141 caggcgcagg gcgacctctt tgagcagttg atcgccgacc gagtggccta agctgtcgtt
    16201 gataatcttg aagcggtcca gatcaacata gaggatggcg aatttgcgct cctcgttgcg
    16261 ctgcgcgcgg gcgattgcca gttccaggtg atctatgaac atggcgcgat tcggcagacc
    16321 cgtgagcaca tcgtgcatgg cctcgtgttg cagctgttcc tcggcgcgct tgcggtcggt
    16381 gatgtcctga atctggaaga cgagcggcgt cgaggcgacg tgcgggccac ccgccagaga
    16441 cgcgctccac agtccccaca ccttatgtcc ggacttgtgc cggaagcgca cctcgcactg
    16501 gtcaatcgtg ctgttgcctt tacgcagacg cttgatggcg tcaaccgcca cgccgagatc
    16561 atccgggtgg atgacggtca aaaagtctat gaccagcatc tcgcgctccg agtatcccag
    16621 aatcttacag agcgcgcggt tgacctgaag ccagcgtccc tcggaagaga cgagcgccat
    16681 gccgatggcg gcgaagtcga aagcgttgcg gaaacggtct tcgtcgctcg ccgagttgag
    16741 attgctgctg ctgttgttgg caacagccgt cgtgcccgtg gttgcgacgc tgatgtaatg
    16801 gttatttcta aaagaggcgt aggcgaaata ggcgagggcg gcaaagaccc ctgcgagcag
    16861 ggcggtgttc aggccgatga aatgagtgac cgccgccagt attccggcga cgcctccggc
    16921 catgagatag acgcagagag agttgagata acttttagcc agcgtccgct tgcgcccctg
    16981 ctgcgggctt gcgcttctgc cgtgcagaga aaccgctgcg ctcagaacag attgaaaaac
    17041 gacgagggcg gcgagcgcgg tcaggatggc gggaacggat tgagcttcga ggagttgcgg
    17101 caacgaaccg aacccgaagc gcaggagggg tgcggcgagg aagatcggga gcacggagat
    17161 cgccgaacga tagatgagca gcccgacgct cttgctggaa tgaagcagca cgcagggcgt
    17221 gacgacgaac gcgagcacca ccgccgcttc ggcgtcgaac agcaggaggg agagaagtaa
    17281 gaaactatcc gtgaggtaga gatgattgcc gaggatagtg ttcttcacgg ggaaggccgc
    17341 gcaggtatgc accgcacaga gggcgaacag cagaagcggc agtccgagat atgcggtcgg
    17401 taaattgtaa atagccgcta aaaagatgag cgcatctaca aataagatta cctttgcgta
    17461 gaggttgata tttttttgtt cgaaggaact tttcatgccg ccctgatgtg caacttgaag
    17521 ctggagtttg agcggaggca agcctttagc ttggcgtccg aactgtcaac taggcaattc
    17581 aaatgccacc tagcccgcac acgagaacgc ctccgattcc cgccaaactc gtgtccacgc
    17641 gacaggacgg ttcgcgcttt gtgccatcac ccccattcaa gttgacttga gcggcagagc
    17701 agattgacag aaattacatc acggtcgagt ctcgctccgc catgcgtggc gaaagcgcga
    17761 cgaacgaatc gcgctcgact ggcctgaaag cggcgcgcct ccgttcgacg gcgcgctccg
    17821 ctgctgccgt cgctgtcatc gctgtcgttg cggataagaa atgtgacaac atcgagcggt
    17881 tttcgcgtta agatagtttc ctaatgatcg caacacgaat ggacagagtg agccgtcgaa
    17941 gggtcttcgg aagcaaggcg ggcggcgggg tctgattaaa gttctgtgat attcgcaaaa
    18001 cggggaaggg gcagaaaaaa ttatgcgttg gcgaggcagg cgcgagagta cgaatgtgga
    18061 ggatcgcagg ggcgtcgggc gcggcgggct ggcggtcggc ggtggactcg gcacgattgt
    18121 cttgatcgtg ctcgcgctcc tgttcggcgc agacccgcgt gagttgctcg atcagagcgg
    18181 cgggcagaac cccgcgcagg gcacgcaaag cgggcgaccg gctaactcgc aggaagacga
    18241 actcaagcag ttctcgtcgg tcgtcctcgc cgagacggaa gacgtgtggc agcaactctt
    18301 tcaacaagcg ggcaggcgtt accgcgagcc gacgctttcg ctcttcaccg gacagacgcg
    18361 ttcggcctgc ggcgcggcgg gcgcaagcac cggacccttc tactgttcgg gcgacgagaa
    18421 ggtttacata gacctctctt tctatcgtga tctgcggacg cgcctcggcg cacccggcga
    18481 cttcgcacaa gcctacgtca tcgcacacga ggtcggccat cacatccaac acctgctcgg
    18541 aacgatggag caggtggacg cgcagcagcg caggatgagc gaagcgtcgg ccaaccgtct
    18601 ctccgtcatg ctcgaactac aggcagactt ctacgcgggc gtctgggcac actacgcgga
    18661 gcgtcggggc atcatcgaac cgggcgacat cgaagaggcg cttaacgcgg ccaccgccat
    18721 cggagatgac cgtctacagc agagaggcca gggctacgtc gtccccgatt ccttcacgca
    18781 cggcacttcc gagcagcgcg tccgctggtt cagaaaggga tacgagacgg gcgacatgcg
    18841 gcagggcgac actttcagtg caaggaatct ctaactcccg ttatgcagtt ggaaatacgc
    18901 gaggcgcgca cgaaagatga gttagagcgg ctgttcccgc tgctgctgtt ggcagagcct
    18961 tcccggagcg cgctgcgttg gagcctgcgc aatctgagcg acacggccta tcgcatggac
    19021 gttgacggag agttggcggg cgcggccacg atgcgctggc gacgcgaccc ttgcgagatg
    19081 gttgaactgg ccgtcgcgcc tgagcggcag ggcgaaggac tgggaaagaa gtttgtcgcg
    19141 tggctcgtcg cggaggcgcg ccgtcgcggc aaggcggaga tcatcgtggg cacgagcagc
    19201 acgagcgcgg ccaacctgat cttttatcag aagtgcggat tcagagtaga ccacgtgcga
    19261 caggattact tctggtatta cgacgagccg gtctatgaga acggactgcc cgtgcgcgac
    19321 atgctcgtct ttcgccaaga ccttgccgga gcgtttgcgg gcgagacgac ggagcaggcc
    19381 gccacagacg attcctgaag attaactaac gcagccacga agcagaggag acaaaggttg
    19441 gaacgcatcg tcgagtgtgt gccgaacttc agcgaggggc gcaggccgga agtcatcgag
    19501 caactcatcg ccgccgtcga gtcggttgac gggacggcgg tgctcgatac gcacgtggat
    19561 gcggatcata accgttcggt catcacgttt gtcggcgggg cggacacgat tgtcgaggcg
    19621 gcggtgcgtg tcgccgcacg cgcggctgag ttgatcgatc tgcgacagca tgtgggcgtc
    19681 cacccgcgcg tgggcgcgct cgacgtgctg cccttcgtgc ccgtgcgcgg cgtgacgctg
    19741 gaggattgca tccatctcgc gcacgcggcg ggagcacgca tcgcacgcga gacgggcgtg
    19801 cccgtctatt tttacgagag cgcggcgcgc aagcccgacc gcgtgaatct ggaagacgtg
    19861 cgacgcggcg gttttgaaca gttgcgcgaa gagattggcg tgaatcccga acgcgccccg
    19921 gacgagggcg cgccgcgcgt gcatgagacg gcgggcgcgt gcgtcgtcgg cgcgcgttcg
    19981 ctcctcatcg cctacaacat caacctgcac acgacggaga tcagcgtcgc gcgtcgcatc
    20041 gcacgcgccg tgcgcgggcg cgacggcggc ctgcgtttcc tgaaggcgct cggcttcgag
    20101 ttgcgcgagc gtggcctgac acaggtttcg atgaacctcg tgcgctttga aaagagcgca
    20161 ctccatcacg ccttcgaggc cgtgcggcgc gaggccgaac gctacggcgt gcgcgtggcc
    20221 ggtagtgaga tcgtcggcct catcccgcag gccgcgctcg accgcagcgc ggaatatttt
    20281 ctccagatcg aaaacttctc ccccgaactc atactcgaaa accgcatcgc ctccgcgctc
    20341 gccagaaggc gagaggccgc acgccttgaa cgccggagag acgatgccct tgccgtgagt
    20401 tggagcgcgg atgtcgaagc ggaagccgtc gcgcggcgcg gcgtcatgcc ggagcatgaa
    20461 gttaagagaa ctgctaacga acgctcgccc cgcgccccgg atgcttcgac gcacgacgcc
    20521 gcaacgacag tgccggaaga gatcacgggc ggcggcgcgg cggcggcgca cgcggcctcg
    20581 ctcgcggctg cgctcggcga gatggtcgcg catctggtcg agcgcaaggg caagcaggag
    20641 gacatggaac gcgaagcgcg cgacgctttg caggcgttga gcgatctgcg cgcgcggctg
    20701 gcggaagcgg cagacgcgga cggcgcgagt ttcgcgggag tgctcggcgc gcgtcgcatg
    20761 ccggggcgaa acgacgaaga gcggcgggcg cgcgagaacg ccctggaaga ggctttgaag
    20821 ggtgcggcgg cggtgccgct tgagacggcg agcctcgccg tgcaggtcgg cgagttgttg
    20881 gagacgctgg ccgaactcgg cgaaccggcg tggctctccg acgctgcgac gggcgcgcaa
    20941 ctcgcgctcg cctcgacggt cgccgcgcgt tacaacgtgc tcgtcaacac gtctgaatta
    21001 gaggatgaag agtttgcctc ggaacacgtt tcgcgcgccg acgatctgct cgaacgcgca
    21061 cgcgagatcg ctgcgcgcgt cgaggtgatg ctgctggaat caatcggaag atagagcgga
    21121 ggcgagaaag cgagtatgga aaacgagaga agagaggatg cgcgggcgcg cgtctccatc
    21181 gaagcgtggt gggagggatt gtcgggcagg caccaggcgc gcgtctcaga cctcagcctc
    21241 ggcggttgct tcatagactc gctcggcaaa gcagagatcg gcgaactcat cgtcttcgcc
    21301 atcaagcaac ccgacggcgg gtggctccaa ctgcgcgggc aggtcgcttc cgtagatcag
    21361 cacgtcgggt tcagtctcgc cttcacctat ctcaccgaag acgagcaaca cgcgcttagc
    21421 gaactcatca ctccttgaga cggattcgac gctcgccgga tgtccggctt acttgttgcg
    21481 cgcgctctgc ctccaactgc gagagatcat attgcgggac gcaactcatt ctcttccgtg
    21541 tcagcctccg gcgttcttca taaaaattat ctttagctag agatgtttgc ggaggaaggt
    21601 gacgatcttc cagccgtcga tatatttcca gggcgactgg ccgagcgtgt agtggccgca
    21661 gcgcagccag acggcgtcga gcggatgccc ggctttgcgc acgccctcaa tcgcatcgcg
    21721 cgagaggtct gcggggaagg tgagatcgta gcgcgcggcg atgaaacgaa tcgggcgcgg
    21781 cctgaggtgt ttgagttttt ccgaataggc gagcgggctg atgggtagcc agtagtggcg
    21841 cagttcgtcg agcgttacgg agtctgcgaa cccggcgcgg acgtgcgccg tggagatgcc
    21901 gcgccacgct acgtcggcca cgtagccgga gacgtggttg aagacgcccg tgcagacgag
    21961 cggctcatgc acgagcgaga gaaagccgac gcacgaaccg atgctcgtgc ccatgatccc
    22021 tatgcgctcg taaccctgtt cgtgcagcca gcggacggcg gcgcgcgtgt cgagcacggc
    22081 ctgacgcatg gattgcagcg tgcgcccgat gttggaagag acgagatgat ctgcgcgttc
    22141 gagttcgggc gtgcggcgcg cgtcgtggta gggaagcgac aggcgcaggg cggcgatgcc
    22201 gaagcgtgta aagacgtggc ataggtcggc gtagctgtcc ggctgcgcgt tccagtgcgg
    22261 cagaatgacg acggcgcggc gcgtgtcgtt ctctttgccg gtaatggttg actcgcggaa
    22321 gaagcgcgcg tgcgccgtgt tgttttccgg cgcgggcgtc tcaattgcgc tcgtccacgt
    22381 caacctttga ccgtcgagcc ggtaatcgtt aatgggaggg agcgcgtaaa actcttcact
    22441 gcgggcgacg gcctctgcgg cataggcgga gaggatcagg cgcgggtcgc cgccgttggc
    22501 gtgcggagcg atgaaagacg tgccccactc gaaagggcgc acgatgcggt tggtgtcgcg
    22561 cgtggcgaaa tgacgttcct tgatcagtat ccggcgtttg agcaacttcg cttttgacct
    22621 ccgtaaaaaa tttaaggtta acagagcgaa aggtcaaaag gcgaaaggcg gaggaacgaa
    22681 aggcgaagga ctgaaaggcg agggcaaacg taaaagggaa gagggaaagg aaaagacgaa
    22741 cacgtcgtcg cttctccttt ccctcttccc ttttaccctt tacccgacgg agcggatttc
    22801 agggttgagt gttagacgac accgcacagc gaccatgcct gtgcgacggc gttgtagttc
    22861 gtgctgcccg atccgtagag cgcagccgcc gcgttgagcg tggccgtgcg tgcgccgcgg
    22921 aagttggtgc tcgaagtcat gtaggaggtg agcgcgcgat accagatgcg cgccgccgcg
    22981 tctgcgccga tgccggtcat cgaaccgccg cgatggtgcg tgccgccgac ggccacgagg
    23041 tagaaagcct tgttgccgat gccggagttg atgtggacgc cgccgctgtc gcccgtgccg
    23101 gtgtaacgtt ccgcgtagtg atcgggatcg tcgtcggcgg tgaagccgct gttgctggcg
    23161 gagtgggggt tgtccatgta gcgcagggcg tcgcccgcca cgcccggcgt gtagcattgc
    23221 tcgccgatct tccaggtgtt cgaggtttgc ccctgcgtgt agcgttcgac catcgccccg
    23281 aacacgtcgg acatggactc gttgagcgcg cccgactcgc ccgaataggt gagccgcgcc
    23341 gtgcgctcgg tgatgccgtg cgtcatctcg tggccgcaga tgtcgagcgt gacgagcggc
    23401 gagaaggatg aaccgttacc gtcgccgtag gtcatgtagc tgccgttcca gaaagcgttg
    23461 ttgtagccca cgccgtaatg cacgactgaa ctgatgaggc cggtgacgcc atcggcggaa
    23521 gtgtaataga cggggccgcc cgcgccgtca atgccgcgcc gaccgtgcgt gttgtagaaa
    23581 tagtcgtagg tcttgcccgc gccgtaatgc gcgtcaacgc ccgcgcgctg cgtcgaagtg
    23641 ttccagacgt tgtcggcgtc ggtgaagcga tagacggaag aggtcgtgtt gcgcatgtcg
    23701 aaggtgccga tcttgcgcgt gaggtcttcc atgtaataag agggcgaacg gaagtaggtg
    23761 ttgatcgtca cggtgccgct gtagagcgaa gagccggaag cggtttgcag gttgtcgtag
    23821 ttccacacga cgttgcccgt gtgagcgtca acgaaataga cgggcatgga ggtctgttcg
    23881 gttccgtctt cacggcgcag ttccacgcga taggtcaggt agtcgttgcc ttcgtggcgg
    23941 aggatctgca tgtcggcagc gggcggcgcg gtcaggcaat cttcacaacc gagatcgctg
    24001 acggctgtgc gcacggcttc gtccggcgtg cggtgcgggg tggtgtcggc ctgcacggag
    24061 tcgatgaggt cgtccgtgac ggcgaagacc gaatcgtccg ggttgaggtg aacgatggcc
    24121 tcgccgccga agaccgggat gtcctgatag gtttgttgga agcgcgtgtg cgtcttttcc
    24181 tgatcgtctt tggagacgcg cgtggctttc agttcgcgct tggcatcgcg gatgccgtgc
    24241 ttttcggcgc gggcggcgat cacgtcatgc gcgatctctt gcgagcgcgc cagtttggcc
    24301 gcagccttgt cggcagattt gtcggctgac gcggaagcgt cacgacgggg gatggtgagg
    24361 gcgaaaacga acgcgaggga gatcgcgata acgaagatgg gttttttcat tgattctcct
    24421 tgattgagtc tgagtgtttc gggtcgtgaa gagacggagc cgcaaacctt gcgcagcccc
    24481 gcgagaggga acgaggacgg gagtagagat gaaacagccg tgccgacttg aatagaatga
    24541 attgagcaca gaaataccgc gagattagtg cgtcaaccgc tgcctgattg tttaaaagag
    24601 acgccgtcca gaattgagga gaaacttgtg cggcgttaag gtcaaaaaca attgtaataa
    24661 ttttactgcg acttggggga ggcaattatt agcagcttat ctatacgagg gcaagacttt
    24721 tgatctggcc gattttgcgt tggaacaaaa aaatgacatc tcgctttgct ccggcgcgga
    24781 cgttccgcgt tgagacgtaa agttggaccc cggaaacgag aaaggcgagt ctcacctgag
    24841 actcgccttt cttctaaccc ggatacgtcg cgcctgcgtc gccgcgacgc ttaacggctg
    24901 cgcgggtatg cgcccgtgtc gtccggttgc cagtcaacga cgggctggat ggcgggattg
    24961 tcacggcaga cccagacgtt agcgcgcatg ccgctcttca agaccttact tctccgctcg
    25021 atgcgtcgcc aatcgctccg cgtgaacgcc ttgccgttga tgagatagaa ctccgaaggg
    25081 tcgtccgtcg cggggtggcg gatggtgact tcttcggtgg tgtcgaagtt ggtgcggatg
    25141 cgcaggaaga tgctccctct ccggcgacga taggcaagca cgcgcccgtc ataaagtgtg
    25201 agatcgctac ggtcgcactt cagttctgcc gagggcatga cgcggccacg cgacggctgc
    25261 tgctgctgct gcgcgacgcg tgccccgccc gctgcggaga cgtagctgga aacggag
//
LOCUS       DASWNS010000616         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_412702, whole genome shotgun sequence.
ACCESSION   DASWNS010000616 DASWNS010000000
VERSION     DASWNS010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412702"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /gene="ccsA"
                     /locus_tag="VGB76_21170"
     CDS             complement(<1..907)
                     /gene="ccsA"
                     /locus_tag="VGB76_21170"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY0101450.1"
                     /translation="MSEALHNAEIISSPSEASSPKQEEGSGWSLKSYLPAILPIVGSL
                     LMVFLRIQMGGDRFIDDGALMMIALACYLTAATFHLTNLYAPSNMAQKIGLWTGCLGV
                     FFNLSSWCVRWVAGYDRELQIFLSQGRSAAEMQWAFRFIPFANLYDLSLAFAFGAGIT
                     TMIISGRKNFRFIGALSLPLAALILVLARFIGNDFINLPPVLDSYWRPIHVGIASLAY
                     GVALVCFAAAVIYLLKVGTKPESMAIWAAIFGISVFATVSRFSVFTTGIYTASTFIKG
                     SRTAIPLRADIDFVGQFIVASGVLLL"
     gene            complement(900..>2202)
                     /locus_tag="VGB76_21175"
     CDS             complement(900..>2202)
                     /locus_tag="VGB76_21175"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY0101451.1"
                     /translation="GKLDVFDIYHSWYFNALLLILSLNIVLASIERFPGAWVYIARPK
                     LEVVAAWLRKQPVTHTLEMRGESRAALVERIAAATKSVGYKPIVTEKGNRTFILAQRG
                     TWNRLGAYAVHVALLTIFFGGFLTAQFGRTGQMGLKPGTTSNEMTETIFNTDDPNTFR
                     LDKSTATLPFQVYCTDIRQELIRKDGPITADNTIDWLTQIKIKDETGEHDALVHMNRP
                     YDYRGYRFFQASFINVGHARNIKLSLTPEAGGEPQLVNIARDGSTQLPDGTKIDFVDF
                     QPDFIMQGSQITSQSGEYNKPAAILNVTTPAGERSRAFAFAQELPSGAPIGRAVAGYK
                     FRLVDFEKVPNAHILAIQRDPGTTPFYIGSGLLFLTLSAVFFFSHQRVWAIVEERDSG
                     SKDFDVILGGNTNRNVIALEDRFKKLLQAITGQPQEVKNHE"
BASE COUNT          467 a          623 c          695 g          417 t
ORIGIN      
        1 ccaacagcag cacgcccgac gcgacgatga actggccgac gaaatcaatg tccgcgcgca
       61 gagggatagc cgtgcgagag cctttaataa aagtcgaggc cgtatagatg cccgtcgtga
      121 agaccgagaa gcggctcacg gtggcgaaga cgctgatgcc gaaaatggcc gcccagatcg
      181 ccatcgattc cggcttcgtg ccgaccttca acaggtagat gaccgccgcc gcgaagcaga
      241 cgagcgcgac gccgtaagcg agcgaagcga tgccgacgtg aatcggtcgc cagtaggaat
      301 cgaggacggg cggcaaattg atgaagtcgt tgccgatgaa gcgcgccaga acgaggatga
      361 gcgcggcgag cggcagcgac agcgcgccga tgaagcgaaa gttcttgcgc ccggagatga
      421 tcatggtcgt gatgcctgcg ccaaaggcga acgcgaggct cagatcgtag aggttggcaa
      481 acgggatgaa gcgaaacgcc cactgcatct ccgccgcgct gcgcccctgc gacaaaaaga
      541 tttgcaactc gcggtcgtaa cccgccaccc agcgcacgca ccaactcgac aggttgaaga
      601 acacgccgag gcagcccgtc cacaagccga tcttctgcgc catgttcgag ggcgcgtaaa
      661 gattcgtcag gtggaaagtg gcggcggtca ggtagcaggc gagcgcgatc atcatcaacg
      721 cgccgtcgtc tatgaagcgg tcgccgccca tctgaatgcg caggaagacc atcaacagcg
      781 agccgacgat ggggaggatc gcgggcagat acgacttgag cgaccagccg ctgccttcct
      841 cttgtttcgg agaactggct tcggacggac tacttatgat ttccgcatta tgtagagctt
      901 cactcatgat tttttacctc ttggggctgc cccgtgatgg cctgtaagag tttcttgaat
      961 ctgtcttcga gcgcgatgac gttgcggttg gtgttgccgc cgaggatcac gtcgaaatct
     1021 ttcgagccgg aatcacgctc ttcgacaatc gcccacacgc gctggtgcga gaagaagaag
     1081 accgcgctca gcgtgagaaa gagcaggccg ctgccgatgt aaaagggcgt cgtgccggga
     1141 tcacgctgga tggcgaggat gtgcgcgttc ggcaccttct caaaatcaac cagccggaac
     1201 ttgtaaccgg cgaccgcacg cccgattggc gcgccgctcg gcaactcctg tgcaaacgca
     1261 aacgcgcggc tccgctcgcc cgccggagtt gtcacgttca gaattgccgc gggcttgttg
     1321 tattcgccgg actgcgaagt gatctggctg ccctgcatga tgaagtcggg ttggaaatca
     1381 acgaagtcga tctttgttcc atcgggcagt tgggtggaac cgtcgcgcgc gatgttcacc
     1441 aactgcggct cgccgcccgc ttccggcgtg aggctcagct tgatgttgcg cgcgtggccg
     1501 acgttgataa agctggcttg aaagaaacga tagccgcgat aatcgtaggg acgattcatg
     1561 tgtacgagcg cgtcgtgttc gcccgtctcg tctttgatct tgatctgggt caaccagtca
     1621 atggtgttgt ccgccgtgat ggggccgtct tttctgatca actcctgccg gatgtccgtg
     1681 cagtagactt gaaagggcag ggtggcggtg gatttatcca gacgaaaagt gttcggatcg
     1741 tccgtgttga agatcgtctc ggtcatttca ttcgaggttg tgccgggctt caagcccatc
     1801 tgccccgtgc gcccgaactg cgccgtcaga aacccgccga agaagatcgt caggagcgca
     1861 acgtggacgg catacgcgcc gaggcgattc caggtgccgc gctgcgcgag aatgaaggtg
     1921 cgattgccct tctccgtcac gatgggtttg tagccgacgc tctttgtcgc cgccgcgatg
     1981 cgttcgacaa gcgcggcgcg gctctcgccg cgcatctcaa gcgtgtgcgt cacgggctgc
     2041 ttgcgcagcc aggcggcgac gacttcgagt ttcggtcgcg cgatgtagac ccacgcgccg
     2101 ggaaaacgct caatcgaagc gagcacgata ttcaacgaga ggatcagcag gagcgcgttg
     2161 aaataccacg aatggtagat gtcgaaaacg tcgagcttgc cg
//
LOCUS       DASWNS010000617        21516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_101038, whole genome shotgun sequence.
ACCESSION   DASWNS010000617 DASWNS010000000
VERSION     DASWNS010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 21516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21516
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101038"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VGB76_21180"
     CDS             complement(<1..686)
                     /locus_tag="VGB76_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101452.1"
                     /translation="MQTNTPAASRLSLSPLGAGDLIDRTIRLYRRHFLTLIRTAAPPV
                     VGSATGSVLWTVSMHAIPATESGGRLVLYVGMAVVGGLLLMTGNFFFLLIMGGASRNL
                     VMHLLWDEPVSARMIYRSVRSRFWGLVGALFMIGIFAWVVGMIIFFTWLIAVAMVVGG
                     ALAIGGGAMLLGGILGVLLFILLTTGALFLFFFIVGRAAYVPQVMLVEGRGVFDAIGR
                     SVTLSRGNVRR"
     gene            complement(912..1829)
                     /locus_tag="VGB76_21185"
     CDS             complement(912..1829)
                     /locus_tag="VGB76_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101453.1"
                     /translation="MKRLGWFTVLACVLLFSSLTYVVAPAAHTPRPLLAQGKAFDPGC
                     PTLPFEAIKMKRPIDDACPRGGSAASTDPTGAHALENLAKNNFCVKGDPVPVTYNAFV
                     GLQTAVNNMPDLPWGEGTRLPPDRSKLLGLKIKDSGRSLTLGEGTKVVFVGYVMDAKH
                     DDTAKGEDVNCKIPGNESNDIHISLRTVPGAQAAAPKGKPDLRCNSITAEISPHFRPA
                     VWDKFDSAPYRAVFQKYPVRITGTLLFDAAHRPCENGIPSAGNPVRISVWEIHPVYAI
                     DVCKYPTITRCKVGDQSAWTPFDKYKLGS"
     gene            complement(1885..2046)
                     /locus_tag="VGB76_21190"
     CDS             complement(1885..2046)
                     /locus_tag="VGB76_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101454.1"
                     /translation="MLYSVARAGEGTATGRETCRGVLTQRQRGSEAVPPSARRPQSKS
                     NLKLSVAPG"
     gene            2461..5925
                     /locus_tag="VGB76_21195"
     CDS             2461..5925
                     /locus_tag="VGB76_21195"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0101455.1"
                     /translation="MRSKVLILLVAIFSFSLTATAQTSRGTVSGTVTDPAGAVVSGAN
                     ITLTNTSTGLSRTTNTNEEGLYRFEAVDLGTYTVAIEATGFGALTKTNVIVNANQTSA
                     VDAQITPGGQQVTIDVIADAGAALQTEAPVRGGNISQRQITELPTGSRNPVGLALTLP
                     GVTTNRTGVGISTFVVNGARGRSNNFLIDGVENNDISVAGQGFEINNPDAVQEVSVQT
                     SNYDAEFGRAGGGVVNVITRSGENEFHGTLSTVLDSSRDDAITSSQARNAAVIQRGYP
                     LFFMDNIYSATLGGPLHLPRFGEGGPAITNGKDRTFFFTAFQFQRQRSNASVNLVTPT
                     AAGRATLRSLFAPGANANVDFLLNTTAETVGTANPLQLALGVAPGASGTSSTACTQAA
                     LNAGNRPCVEFATFVRNYASVYDEYEFQFRVDHKIGNNDQLSARFLRETQAQPFGGGA
                     NFPGFDADYSAEFYNFLISETHVFSPTLTNEVRLSYNRIKYLFPIQDPSGLGGRTPNR
                     TVTSISLPQVASNIPQGRTANNYVIQDTVSKVMGNHTFRGGVDYLRQISTQLAPNNQR
                     GSLNYAASTGYTSFANFVDNFGGQNGAAARDFGEPVYFPSMHRIATFFQDRWKATSDL
                     TLTLGLRYENFGTPFNTLRTPAYTGLFNVNPVTLAGPYSQPNQVQRDNNNFAPTVGVA
                     YSPSFTNGFLGSFFGDKKSVIRAGYQIGYDSFFNNIASNAAASSPNVIQTTNTSLVTA
                     ANPRGFANFSNQFPTTAAALSPFSPQTLIAPDLVNPYYQRWSLGFQRELPFNLVMDAS
                     YVGSKGTKLYINEDANPLVAIPALRRPVPAGFTGTPQARLDPLQGARTVRTNGGSSSY
                     HAGQVEVRRRFRNNFQVTGAYTFSKNLSNADEVFATGVGTASSVFALPAVFGGDPLEK
                     GLSQNDRTHRAVFTYVFEVPFMREQRGFLGRLLGGYQLSGITSFESGTPYTVFNGFDS
                     DGLGGGNERPTYNPQGQAGVRAVPVVDARQTITVNGQNVANPQFGAITHYVNPDAVTG
                     FTAAGAPIFGNPTIDPLTARYIVNPAYIPGAPMSIPRFGNTPRSSERSAGINNWNLFV
                     QKNTNITENTRLEFRAEFYNVFNHPQFDTVGTSNAGTGVAGRFLRTDTVGTTGGGRVI
                     KYQIKYRF"
     gene            complement(6037..7377)
                     /locus_tag="VGB76_21200"
     CDS             complement(6037..7377)
                     /locus_tag="VGB76_21200"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEY0101456.1"
                     /translation="MYEFAVTPAITQIRRRGVEVSEVAPESLAAELELEAGDRIIRVN
                     GRLVRDYLDFRFQTGGETELTLEVLKRNGEEWQLEIERGEGEDFGLQFENIVPRQCAN
                     ECLFCFCHGNPPDARPALSFRDEDVRLSFLYGNYTTLTSITEAEMRRVIEQRLTPQYV
                     SVHATDLDVRAYLLGVDKKRADIRGKMRRMLDAGIEIHAQVVLCPEINDGEILARTVR
                     DLSADYPTLRSVAIVPLGLTRYNTDARLTPVTPEFCRDTIRQVKLLQRELHARLGTNF
                     AFLGDEFYLRAGQPVPSRRHYGDYPQIEDGVGMVRSFYEDFRRLERKLDARAPARPSE
                     LDGTILTGRLFAPVLRDLIARLNERCGTRFRVVAVPNDYFGPDIVVAGLMTGRDVLAV
                     REQIEGSFVILPSVAFKSDEPVMLDGTTHAELQQQLGLPVRALDFEGFARLLSA"
     gene            7813..8247
                     /locus_tag="VGB76_21205"
     CDS             7813..8247
                     /locus_tag="VGB76_21205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0101457.1"
                     /translation="MPETTLDSQTSPATLPPVSARKPTVLLVEDDAAVRRYLEVILRR
                     ANYEVLSASNGIEAIKTAHSSKLDAVVTDAMMPHLNGHELCRYLRARPELARLPILLL
                     SGLEPNAAAPERSEHADAHLTKPVRPEVFTDCLARLLAGATL"
     gene            complement(8362..9501)
                     /gene="ald"
                     /locus_tag="VGB76_21210"
     CDS             complement(8362..9501)
                     /gene="ald"
                     /locus_tag="VGB76_21210"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY0101458.1"
                     /translation="MLIGLPKEIKDNEYRVGMTPAGVRALTDAGHKVVVERKAGEGSG
                     FDDALYERAGATMLETADEVWAQGEMIVKVKEPIVPEYPRMREGQVLFTYLHLAPDHE
                     LTKQLLERKVTGVAYETITDRKGTLPLLTPMSEVAGRMAIQVGAQYLEKMSGGRGVLL
                     GGVPGVPAAKVVIIGGGVVGTNSAKMAVGLGAHVTIIDNNLDRLRELDDIFLSRITTL
                     ASSAYAIQGAISEADLIVGAVLVPGAAAPKLITREMLKKVPNGAVIVDVAVDQGGCIE
                     TTHPTTHSDPTYYVEGVLHYCVANMPGAVPRTSTFALTNATLPYTLKLANKGFLSAVA
                     SDPGLKEGVNTYAGKLTYEAVAQAQGLDFTPLDNILSASGAAAKR"
     gene            complement(9612..10076)
                     /locus_tag="VGB76_21215"
     CDS             complement(9612..10076)
                     /locus_tag="VGB76_21215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101459.1"
                     /translation="MLLREPYEPRPIRFLEEWQQAGWRLKVYGISYGRVRAREELIET
                     ARRLARESLPTAHEGGNYGVGYVGVHDGRGANFIFVDWWADENELHHRVFAAPSDELK
                     DLPEITATGLSVCVWDLRVQCFEREAWLEAVLKNTSGADLEDYMRRRLNEDA"
     gene            complement(10169..11761)
                     /locus_tag="VGB76_21220"
     CDS             complement(10169..11761)
                     /locus_tag="VGB76_21220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0101460.1"
                     /translation="MAQAKKALSRTTKKSAGRSSSNGNGKQGARQSVKTYSNYIAGEW
                     LPAASGEYFENINPADTRDVIGRFPVSNAEDVERAVAAAKSAADGWRRMPAPRRAEIL
                     FRLGNILIRDKDRHAADMTREMGKVLKETGGDVQEAIDCTFYTAGEGRRLHGYTTPAE
                     MPDKFAMCVRQPVGICGLITPWNFPMAIPSWKLIPALVCGNTVVIKPAEDTPLSTYNL
                     VKACEEAGVPRGVVNLVTGYGAEAGKPLTEHRDVRLISFTGSTETGRLIAGLCADRNA
                     ICSLELGGKNVIIVMDDADLDLAVEGAVWGAFGTSGQRCTASSRIVVQKKVYKKFTQK
                     LVERAESLRVGNGADASTDMGPVINAAAVHKIMGYIEVGEREDGARLATGGSRLDTGA
                     HANGYFIEPTVFTDVAPGMRIAQEEIFGPVTAVIPANNLEEAVEIANGVRYGLSAAIY
                     TQDVNRAFHAMNDMYTGIFYVNSSTIGAEVHLPFGGTKATGNGHREAGTQVLDIFTEW
                     KSVYVDYSGKLQRAQIDEVKFE"
     gene            complement(11848..13470)
                     /locus_tag="VGB76_21225"
     CDS             complement(11848..13470)
                     /locus_tag="VGB76_21225"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEY0101461.1"
                     /translation="MYQLPQGQTNEAERAILRRLRVWIAALALACLVVGVGVGAMLAG
                     RPAVAQNETQRAAQNARAPEALSASFAEIAQRVEPAVVNIDTVSAPQVAERGGTGSGN
                     GNGNSNNDSDDDSDDKDGGGDNPLMEMFRRQMPQRPSRGVGSGFIVDSKGYILTNHHV
                     VEGATRITVRLQSGELMRGRVVGEDDETDLAVIKVETSRELPTVKLGNSDEAQVGDWV
                     LAVGSPFGLDQTVTAGIISTRERTTPVSTSFQRFLQTDAAINRGNSGGPLVNMRGEVI
                     GINSQIATSTGDYNGIGFALPSNEASFVYRQIVDGGRVKRGYLGVLLDSVKAEFARVY
                     GLKDAKGAIITDVREGPARAAGVQVNDIIVEFNGKPIANSQDLINKVASTPVGETVQL
                     TYLRDLNGQLERRTVGVSVGERPPRGASDALPTPTPSPSKATPAKPEGERPTLGLTLS
                     ELTPQLANERNLKGVRGLLVRDVDQSGLAYEAQIVENMVIQRVNRIPVNTLAEFERVI
                     SGLKVGDAVVMNVTFLDRRNLITQTIIQFTYQ"
     gene            complement(13702..14676)
                     /locus_tag="VGB76_21230"
     CDS             complement(13702..14676)
                     /locus_tag="VGB76_21230"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0101462.1"
                     /translation="MRLKSNLAADGALMLTTLIWGSTFFMAKDILTVWPPMAYMFVRF
                     AAAALLLAVMFPQQLARARREEWRAGATLGVLMGFGFGLQAAGQVYTTASKSAFVTGL
                     TTPLVPFVAYLILRARPSVENLIGVAFASLGGLLILAPQGADGTINFGDLLTLAATAL
                     FATHITLMSVYARRYDVRQLTVLQIASTACLFTFIWAGLQAWNFIAGADAMPQAFARE
                     TAPLVWSGRVVWQLVYLATVATVVSFLCWTWGQARTTATHAAIIFSLEPVFATLFAVA
                     VIGAGEWMGTRGFVGAALVFAGIIISEMRWSERRERRANEKAREAEAA"
     gene            complement(14866..15837)
                     /locus_tag="VGB76_21235"
     CDS             complement(14866..15837)
                     /locus_tag="VGB76_21235"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0101463.1"
                     /translation="MKALRFENGRLGLAEVGTPRGEAGEALVRVTLAGVCNTDLEIVR
                     GYAGFAGTLGHEFVGVVESAPGAPQLEGQRVVGEINAGCGLCELCVGGDARHCPRRTV
                     LGIKERDGAFAEFLRLPPVNLLPVPDEIEDARAVFAEPLAAACGITERVEITEGMRVA
                     VVGDGKLGLLCAQAIKAQCAGASVTLLGRHEKKLDIARARGVETALVEASGDDLTAQA
                     FDVVVEASGAASGFALALRLTRPRGALVLKSTFHGATEIDAARIVVEEISIVGSRCGR
                     FAPALELLRRDAVDVASLITEEFALADGLRAMQRATENGVLKVLLKP"
     gene            complement(15923..16462)
                     /locus_tag="VGB76_21240"
     CDS             complement(15923..16462)
                     /locus_tag="VGB76_21240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101464.1"
                     /translation="MKTNTSPAHQLLVAVLRLALVVALVGAGWLIYRRLPHDDTTPAA
                     GLGTDRQARDTLLRIILPPHTGMDSAKAAAINSVTAVQLYSIDVAAAQREFFDERRPG
                     MRFEDFIVQRMGARQPITTKLDAQGQTIVYVPPGRWWIHATQGGAQEITWRLPVNVAG
                     REQTVELTNANAYTRTKEF"
     gene            complement(16501..16749)
                     /locus_tag="VGB76_21245"
     CDS             complement(16501..16749)
                     /locus_tag="VGB76_21245"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY0101465.1"
                     /translation="MEVKVYGADWCHDTQDTLKHLKELGVAHAYINVEEDAKASEWVK
                     QHNEGKERKPTLDIAGQVLSVPTNRELESALREKGFMA"
     gene            16894..18363
                     /gene="purF"
                     /locus_tag="VGB76_21250"
     CDS             16894..18363
                     /gene="purF"
                     /locus_tag="VGB76_21250"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY0101466.1"
                     /translation="MRVQLRNFLQQIFPVTTNGTDKFRDECGVFGIYGHTEAARLAYL
                     GLYALQHRGQESCGIVASDGRELRSERAMGLVSDVFDAERLNRLPGASAIGHVRYSTA
                     GEVSIREAQPFLVACQHGQVAICHNGNLPFATEERKRLERAGAIFSSTSDTEVILHGI
                     ARSQAAGINEAIPEVLRETEGAFSMLFLTPDSLVAIRDPRGFRPLALGRLGDNTWVVA
                     SETCAFDLIEARYVRDVEPGEMIIIDKGGLHSSYPLPPREHSMCIFEHVYFARPDSLI
                     YGHSVNESRHKMGKRLAVEQPTEADIVVPVPDSGVAAAIGYAAQSGISYRQGLVRNHY
                     VHRTFIEPKQSIRSFGVRIKLNPVRHLIEGRRVVLIDDSIVRGTTSKKIVRMVREAGA
                     TQVHMRISCPPTISPCFYGVDTPNRDDLIAAQMSVAEIREFIGADSLGYLSLEGMLAA
                     TGISPAASCVACWTGNYPTQITREAETQWAREHEPVPSD"
     gene            18388..18675
                     /locus_tag="VGB76_21255"
     CDS             18388..18675
                     /locus_tag="VGB76_21255"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEY0101467.1"
                     /translation="MRIITRKRLNDFAVRYPETRTGLAHWYQLMKQSSFASFAELRAI
                     FPAADQVGKLTVFNIGGNKARLVAAIHYNRRRIYVRAVLTHQEYDQNRWKE"
     gene            18679..18912
                     /locus_tag="VGB76_21260"
     CDS             18679..18912
                     /locus_tag="VGB76_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101468.1"
                     /translation="MQNRELDKTLSAWPLVSQKIYVPHTEEQYERIVALLDQLIDEVG
                     EDETHPYSSLMELLGVLIEHYETEHVTELTIDQ"
     gene            18942..20039
                     /locus_tag="VGB76_21265"
     CDS             18942..20039
                     /locus_tag="VGB76_21265"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY0101469.1"
                     /translation="MAQSRSIRGCGCVLVVAAIIVGVALFSYFRFYRPAPGPQLPPPS
                     GDELSIHVLDVGQGDAILIVAPGGKTALVDASVPGSGKKILAAMGRYGVDHIDLLIST
                     HAHADHIGGADEVIKGTKVYKVLDSKVPNTTKNYEDFLDAIEANKVEFIGAQPGQTFD
                     LGSGAIISVLAPIQPLFTKDQLRSGGNEPNANSVVTRLDYGEFSILLTGDAEAQTERR
                     LMEQGARLEAKVLKVGHHGSKYASSEDFLNAARFKDALISCGADNRYGHPSQEVLDRL
                     KAAGVKIYRTDLQGEITIKTRGREDYQITTERQADATEIATGRKAQKDDSAKSGFIQY
                     GDFGPAPKASPKAGAANSNRANSNRANSNSR"
     gene            20045..20392
                     /locus_tag="VGB76_21270"
     CDS             20045..20392
                     /locus_tag="VGB76_21270"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 family protein"
                     /protein_id="HEY0101470.1"
                     /translation="MTERTDLSPPGRADEIEVRAVIDRIEDNDVAVLMLEDEAESQLD
                     VPRGALPEDANSDGDHLILKFDVDAESGERTSLKSIKAAPAARAAAADRIKKMQERLT
                     RMSGADDKKDFKL"
     gene            20500..>21516
                     /gene="purM"
                     /locus_tag="VGB76_21275"
     CDS             20500..>21516
                     /gene="purM"
                     /locus_tag="VGB76_21275"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY0101471.1"
                     /translation="MTKPITYSDAGVSIAAANAAVDKIKEMARSTFNARTLSEIGSFG
                     GMFDAAFPSMSAPVLVASADGVGTKLKVAFETNTHNTVGRDLVNHCVNDILVQGARPL
                     FFLDYVATGVLSPEVIAAVVEGMSRGCRENNCVLLGGETAEMPGFYAAGEYDVAGFIV
                     GIVDRERVIDGKRIRAGDVLLALPSVGLHTNGYSLARKLFFEVAGYDARTRLAELDMT
                     VGEALLAPHLSYLPALEGLLDTGALKGLAHITGGGLIDNIPRILPEGTAVEIKRGSWP
                     VLPVFTLMQRIGNVSETEMHRTFNMGVGMVIICAETDAVTIRSHVEARGERIFEIGRV
                     VAGAR"
BASE COUNT         4105 a         6955 c         6440 g         4016 t
ORIGIN      
        1 cggcggacgt tgccgcgcga gagcgtcacg ctgcggccga tggcgtcgaa cacgccgcgc
       61 ccctcgacga gcatcacctg cggcacgtag gccgcgcgcc cgacgatgaa gaagaacaga
      121 aagagcgcgc ccgtcgtcag caggatgaac agcagcacgc cgagtatgcc gcctaacaac
      181 atcgcgccgc cgccaatcgc caacgcgccg ccgaccacca tcgcgaccgc aatgagccac
      241 gtgaagaaga tgatcatgcc gacgacccag gcgaagatgc cgatcatgaa cagcgcgccg
      301 acgagtcccc agaaacgcga gcgcacgctc cggtagatca tgcgcgccga gacgggttca
      361 tcccacaaca aatgcatgac gaggttgcgc gatgcgccgc ccatgatgag caggaaaaag
      421 aagtttcccg tcatcagcaa caaccctccg acgacggcca tgccgacgta gagcacgagc
      481 cgcccgccgc tctccgtcgc agggatcgcg tgcatgctca cagtccacag cacggagccg
      541 gtggccgagc cgacgaccgg aggcgcagcc gtgcgtatga gcgtcaggaa atggcgacgg
      601 tagagacgaa tcgtgcgatc aatgagatcg cccgcgccga gcggggagag tgagagacga
      661 ctggcagcgg gcgtgttggt ctgcatcgga cgagaagact gcggacaggc gcgcgtcggt
      721 tgagttagac gaagaagatt ctagcacacg ccgcaaagcg cgaccgccgc tcccgtcgga
      781 gtcgcggcct cgatcagctt gtccgctgtg cgccctccgt gtcgtctgtg cctctgtggt
      841 gaatgcttat caaagacact taccgcagag acacggagca cacggagggg gcacagagag
      901 ttaagctaag ttcaactccc gagcttgtac ttgtcgaagg gcgtccacgc actctggtcg
      961 cccaccttgc aacgcgtgat ggtcgggtac ttgcacacgt ctatggcgta gacgggatga
     1021 atctcccaga cggagatgcg tacgggattg cctgccgacg ggatgccgtt ttcgcaggga
     1081 cggtgcgccg cgtcgaagag gagcgtgccc gtgatgcgca cggggtactt ctggaagact
     1141 gcgcggtagg gcgcgctgtc gaacttgtcc cagacggcgg gacggaagtg cgggctgatc
     1201 tcggctgtga tcgagttgca gcgcaagtcc ggcttgccct tcggcgcggc ggcctgcgcg
     1261 ccgggcacag tccgcagcga gatgtgtatg tcgttcgact cgttccccgg aatcttgcag
     1321 ttcacgtcct cgcccttcgc cgtgtcgtcg tgcttggcgt ccatgacgta gccgacgaag
     1381 accactttcg tgccttcgcc gagcgtgagg ctgcgcccgc tgtccttgat cttcagtccg
     1441 agcagtttgg aacgatccgg cgggaggcgc gtgccctcgc cccaaggcag gtcgggcatg
     1501 ttgttgacgg cggtttgtaa acctacaaag gcattatagg tgacgggcac ggggtcgccc
     1561 ttgacgcaaa agttgttctt cgcgaggttc tcaagcgcgt gcgcgcccgt ggggtcggtg
     1621 ctggccgcgc tgccgccgcg cgggcaggca tcgtcaatcg gacgcttcat cttgatggct
     1681 tcaaagggca gcgtcgggca accggggtcg aacgctttgc cctgcgcgag cagcggacgc
     1741 ggagtgtgcg cggcgggcgc gacgacataa gtgagcgagg agaacaggag aacgcacgcg
     1801 agaaccgtga accaacccag acgcttcatg tggagcacct caattttgtt tgggtcggcg
     1861 cgccgcagct ttcggcgaag accttcagcc gggagcgacc gagagtttta agtttgactt
     1921 gctctgtggg cggcgggcgg aaggcggcac ggcttcgctt ccgcgctgcc gttgcgtaag
     1981 cacgccccgg caggtttcgc gtccggtggc cgtgccctcg cccgcgcgtg cgacagagta
     2041 aagcacaagc cggacggggc ttcaaattat tcggagcgtt cgactgcgga ggacgagaaa
     2101 cggcggttgt ccatttttct ggatacaaat tttcaaaatt ctggacagct acgtaatcct
     2161 gacgcggaaa gttccttaaa ttattcttaa ttttggcggc agcaatcgtg gcttcaaagt
     2221 tgcagttaac ggttcagcca cattgcttga gctttacagt ctttagagtt tccttcctga
     2281 caaaaacagt atcgcgtgcg ggtcgagtcg gttaggtcaa cgcatctgtt gtgtgcgaag
     2341 caactcgaaa accttgtcgt caataacaca gaaattgtgg ggagcctctt ccgccgaagt
     2401 tgtggaaact gctctcccgg tcgcacatct ctcttaaaaa tctttcagga gaacacacaa
     2461 atgaggtcga aagttttaat tttgctggtc gctattttca gcttttcgct gacagcgacg
     2521 gcacagacga gccgtggcac tgtcagcggc acagtcacgg atccggcggg cgccgtcgtt
     2581 tcgggagcga acattacgct caccaacacc tcgaccggcc tcagccgcac gacgaacacc
     2641 aatgaggaag gattataccg cttcgaggcg gttgatctgg gtacttacac tgtcgccatc
     2701 gaggcgaccg gcttcggcgc gttgacgaaa accaacgtta ttgtcaacgc caaccagact
     2761 tccgccgtcg atgcacagat cacaccgggc ggccagcaag tgaccatcga cgtgatcgcg
     2821 gacgcaggcg cggcgctcca gaccgaagcc cccgtgcgcg gcggcaacat ctcgcaacgc
     2881 cagatcacgg aactgccgac tggcagccgc aaccccgtgg ggcttgcgct gacgctgccc
     2941 ggcgtgacga ccaaccgcac cggcgtcggc atcagcacct tcgtcgtcaa cggcgcgcgc
     3001 ggacgctcga ataacttcct gatcgacggc gtcgagaaca acgacatctc cgtcgccgga
     3061 cagggcttcg agatcaacaa cccggatgcc gtacaggagg tttccgtcca gacctcgaac
     3121 tacgacgcgg agttcggtcg cgcgggcggc ggcgtcgtca acgtcatcac gcgctcgggc
     3181 gagaacgaat tccacggcac gctttcgacc gtcctcgatt cgtcgcgcga cgatgcgatc
     3241 accagttcgc aggcgcgcaa cgcagccgtc atccagcgcg gctacccgct gttcttcatg
     3301 gacaacatct actcggccac gctcggcggc ccgcttcacc tgccgcgctt cggcgaaggc
     3361 ggccccgcta ttaccaacgg caaagaccgc accttcttct tcacggcctt ccagttccag
     3421 cgtcaacgct cgaatgcgtc ggtcaatctg gtgacgccca cggcggctgg ccgcgccacg
     3481 ctgcgctcgc tcttcgcgcc cggcgccaac gccaacgtgg acttcctgct gaacacgacg
     3541 gcggagacgg tcggcaccgc taacccgctc caactcgccc tcggcgtcgc gcccggcgcg
     3601 agcggcacgt catccaccgc ctgcacccag gcggcgttga atgcgggcaa ccgcccctgc
     3661 gtcgagttcg ccacgttcgt caggaactac gcgtccgtct atgacgagta tgaattccag
     3721 ttccgcgtcg atcacaagat cggcaacaac gatcagctct cggcgcgctt cctgcgggag
     3781 acgcaagccc agcccttcgg cggcggcgca aacttccccg gcttcgacgc cgactactcg
     3841 gctgagttct ataacttcct gatctccgaa acgcacgtct tctcgccgac cctgacgaac
     3901 gaggtgcgcc tctcctacaa ccgcatcaag tatctcttcc cgattcaaga cccgtccggc
     3961 ctcggcgggc gaacgccgaa ccgcaccgtc acctcgatct cgctgccgca ggtcgcctcg
     4021 aacattccgc aggggcgcac cgcgaacaac tacgtgattc aggacacggt cagcaaggtg
     4081 atgggtaacc acaccttccg cggcggcgtg gactacctgc gccagatttc gacgcagctt
     4141 gcgcccaaca accagcgcgg ctcgctgaac tacgcggcca gcactggcta cacctctttc
     4201 gccaacttcg tcgacaactt cggcggccag aacggcgcgg cggcgcgcga cttcggcgag
     4261 ccggtctact tcccgtcgat gcaccgcatc gccaccttct tccaggatcg ctggaaggcc
     4321 acgtccgatt tgacgctcac gctcggcctg cgctacgaga acttcggcac gccgttcaac
     4381 acgctgcgca cgcccgccta caccggcctc ttcaacgtta acccggtgac tttggccggt
     4441 ccctacagcc agccgaatca ggtgcagagg gacaacaaca acttcgcccc gacggtgggc
     4501 gtcgcctatt cgccgtcgtt cacgaacggc ttcctcggct cgttcttcgg cgacaagaag
     4561 tccgtgattc gcgccggtta tcagatcggc tacgactcgt tcttcaacaa catcgcctcg
     4621 aacgccgccg cctcgtcgcc gaacgtcatc cagacgacga atacttcgct ggtgaccgcg
     4681 gcgaacccac gcgggttcgc gaatttctcc aaccagttcc ccacgaccgc ggcggcgctc
     4741 tcgcccttca gcccgcagac gctgatcgct cccgacctcg ttaacccgta ctaccagcgt
     4801 tggtcgctcg gcttccagcg cgagttgccg ttcaatctcg tcatggacgc gtcctacgtg
     4861 gggtcgaagg gcacgaagct ctacatcaac gaggacgcca acccgctcgt ggcgattccg
     4921 gcgctacgtc gccccgtccc agccggattc accggcacgc cgcaagcccg cctcgatccg
     4981 ctacagggcg cgcgcaccgt ccgcaccaac ggcggttcgt ccagctatca cgcgggtcag
     5041 gtggaagtgc gccgccgctt ccggaacaac ttccaggtga cgggcgcgta cacattctcg
     5101 aagaatctga gcaacgcgga cgaagtcttc gccacgggcg tcggcacggc cagttcggtc
     5161 ttcgccctcc cggctgtgtt cggcggcgac ccgcttgaga agggtctgtc gcaaaacgac
     5221 cgcacgcacc gcgcggtctt cacctacgtc ttcgaagttc ccttcatgcg cgagcagaga
     5281 gggttcctcg gacgcctgct cggcggatac caactctcgg gcataacttc gttcgagtcg
     5341 ggcacgccct acaccgtctt caacggtttc gactccgacg gcctcggcgg cggcaacgag
     5401 cgtcccacct ataacccgca gggacaggcc ggcgtgcgcg ccgtgcccgt cgtggacgcg
     5461 cgccagacaa tcacggtcaa cggccagaac gtcgccaacc cgcagttcgg cgcgatcact
     5521 cattacgtca acccggacgc cgttacgggc ttcaccgccg cgggcgcgcc catcttcggc
     5581 aaccccacaa tcgacccgct cacggcacgc tacatcgtca acccggctta tatccccggc
     5641 gcgccgatga gcatcccgag gttcggcaac acgccgcgta gctccgagcg ttcagccggc
     5701 atcaacaact ggaacctgtt cgtccagaag aacaccaaca tcaccgagaa cacgcgcctc
     5761 gaattccgcg cggagttcta caacgtcttc aaccacccgc agttcgacac ggttggcacg
     5821 tcgaacgccg ggacgggcgt ggccgggcgt ttcctccgga cggacacggt cggcacgacc
     5881 ggcggcggtc gcgtcatcaa gtaccagatc aagtacaggt tttaactttg cgtgaggggc
     5941 ggaggctcgc agaggcttcc gccacccact taacgaaaaa gccccgcagc cttaaaaaag
     6001 ctgcggggct attttttttg agcgggctaa aagagtttac gcggagagca ggcgcgcgaa
     6061 gccttcgaag tcgagcgcgc ggacgggcag gccgagttgt tgctgcaact ccgcgtgcgt
     6121 cgtgccgtcg agcatgacgg gttcgtcgct cttgaaggcg acgctgggaa gaatgacgaa
     6181 gctgccttcg atctgttcgc gcacggcgag cacgtccctg cccgtcatga gtccggcgac
     6241 gacgatgtca ggaccaaaat aatcgttcgg gacggcgacg acacgaaagc gcgtgccgca
     6301 gcgttcgttc aggcgcgcga tcaaatctcg aaggacgggc gcgaagagtc tgccggtcag
     6361 gatcgtgccg tcgagttcgg acgggcgtgc gggcgcgcgg gcgtcgagct tgcgttcgag
     6421 gcggcggaaa tcttcgtaga aggagcgcac catgccgacg ccgtcttcga tctgcgggta
     6481 gtcgccgtag tgacggcgcg aggggacggg ctgccctgcg cgcaggtaga actcgtcgcc
     6541 gaggaaagcg aagtttgtgc cgagtcgcgc gtgcaactcg cgctgcaaca gtttgacctg
     6601 ccggatcgtg tcgcggcaaa actccggcgt gacgggcgtg agccgcgcat ccgtgttgta
     6661 gcgcgtgagt ccgagcggca cgatggcgac gctgcgcaac gtgggataat cggcggagag
     6721 gtcgcggacg gtgcgcgcca aaatctctcc gtcgttgatc tccgggcaga gcacgacctg
     6781 cgcgtgaatc tcgatcccgg cgtcgagcat gcgccgcatc ttgccgcgaa tgtctgcgcg
     6841 ctttttatcc acgccgagca ggtaggcgcg cacgtccaga tcggtcgcgt ggacggagac
     6901 gtattgcggc gtgagcctct gttcgatgac gcgtcgcatc tcggcctccg tgatcgaggt
     6961 gagcgtcgtg tagttgccgt agaggaaaga gaggcgcacg tcttcgtcgc ggaaggagag
     7021 cgcggggcgc gcgtcgggcg ggttgccgtg gcagaaacag aagaggcact cgttggcaca
     7081 ctggcgcggc acgatgttct cgaactgtag accgaaatcc tcaccctcgc cgcgctcgat
     7141 ctccaactgc cactcctcgc cgttgcgctt caagacttcg agcgtcaact cggtttcgcc
     7201 gcccgtctga aaacggaagt cgagataatc gcgcacgagt cgcccgttga cacggatgat
     7261 gcggtcgccc gcttcgagtt ccaactcggc ggcgaggctc tcgggcgcaa cttcggagac
     7321 ttcgacgccg cgacggcgaa tctgcgtgat ggctggtgtg acggcgaatt cgtacatctt
     7381 aaaatcaact tccccgcgac ggactcacgg cgcacagaac aaaatttgcg cccccggcga
     7441 aacgaacagt atcgtctatt gaagcggcgg attcaactca cgacagcgcg ccgggcgcag
     7501 tcaacctgct gcattctgac cgcccggcct gccagaatga tgcgttttga ccacctctcg
     7561 cacgaagttc ccgcgcgact ttagacgctt gattttccct tcgctgcccg cgcccctttc
     7621 cgaactttaa tcaagtcttc tcctaacata aatttacgag tctcacatga agcggcctac
     7681 ccgcgtcgcc gctccgaccc gatgaagcga cgacgcgggc acacgacttg ccactggaat
     7741 atgcgaagcc gccgcgtctc cccggcgcaa gcgttcgagc cttcgataaa atttcgtatc
     7801 caaccgacga gcatgcctga aacaaccctc gattcccaga cttcacccgc caccctcccg
     7861 ccagtttccg cacgcaagcc caccgtcctg ctcgtggaag acgacgccgc cgtgcgccgt
     7921 tatctcgaag ttatcctgcg ccgcgcgaac tacgaagtgc tctctgccag caacggcatc
     7981 gaagcgatca agaccgccca ctcgtcaaag ctcgacgcgg ttgtgaccga tgccatgatg
     8041 ccgcacctga acgggcacga actctgccgc tacctgcgcg cacgccccga actcgcccgt
     8101 ctccccatcc tcctcctgag cggactcgaa ccgaacgccg ccgcgcccga acgcagcgag
     8161 cacgccgacg cgcacctgac caagcccgtg cgcccggaag tcttcacgga ctgtctcgcg
     8221 cgcctgctcg ccggggcgac tctctgatcc gaactctaaa accggagagg ccgccctgtt
     8281 cgcaggatgg cctctttcat ttgctcacgg acgaaccgac gcgacgctca agagttcgag
     8341 cgcgccgtgc gaactcgaag tttaacgctt cgccgccgcg ccgctggccg agaggatatt
     8401 gtcgagcggc gtgaagtcga gtccctgcgc ctgtgcgacg gcctcgtagg tcagtttccc
     8461 ggcgtaggtg ttgacgccct ccttgagtcc ggggtcgctc gccaccgccg agaggaaacc
     8521 tttgttcgcc agtttgagag tgtaaggcag agtcgcgttc gtcaaagcga aggtcgaggt
     8581 gcgcggcacc gcgcccggca tgttggcgac gcaataatgg agcacccctt cgacgtagta
     8641 ggtcgggtcg ctatgcgtcg tcgggtgcgt cgtctcgatg cagccgccct gatcaaccgc
     8701 cacatcgacg atgaccgcgc cgttcggcac tttcttcaac atctcgcgtg tgatcagttt
     8761 cggcgccgcc gcgccgggca cgagcaccgc gccgacgatc aggtcggcct ccgagatcgc
     8821 gccttgaata gcgtaggccg aagaggcgag cgtcgtgatg cgcgagagga aaatgtcgtc
     8881 gagttcgcgc aggcggtcga gattgttgtc gatgatcgtg acgtgcgcgc cgaggccgac
     8941 agccatcttc gcggagtttg tgccgacgac gccgccgccg atgatcacca ccttcgccgc
     9001 cggaactccc ggcacgccgc cgagcagcac gccgcgcccg cccgacatct tctccagata
     9061 ttgcgcgccc acctgaatcg ccatgcgccc cgccacctcc gacataggcg tcaaaagcgg
     9121 cagcgtgccc ttgcgatccg tgatcgtctc gtaagccacg cccgtcactt tgcgttcgag
     9181 cagttgcttc gtcaactcgt gatcgggcgc gaggtgcagg taggtgaaga gcacctggcc
     9241 ttcgcgcatg cgcgggtact cgggcacaat cggctctttc accttcacga tcatctcgcc
     9301 ctgcgcccaa acttcgtcag ccgtctcaag catcgtcgcg cccgcgcgtt cgtacaacgc
     9361 atcgtcgaag cccgagcctt cgcccgcctt gcgctccacc actaccttgt gtccggcatc
     9421 ggtcagagcg cgcacccctg cgggcgtcat cccgacacga tactcgttat ctttgatctc
     9481 tttggggagt ccgattagca ctttgctttt ctccaatcgt tagaaaattt ggtcgggtga
     9541 aaatcaggga tgcaaacaca ccggcactca cgatccctcc agcgcacggc tacgacgggc
     9601 gacattccgg ctcacgcgtc ttcattcaga cggcgacgca tataatcttc caggtctgct
     9661 ccgctcgtat tcttaagcac cgcttcgagc cacgcctcgc gctcgaaaca ttgcacgcgc
     9721 aaatcccaga cgcacacgga cagacccgtc gccgtgatct cgggtaaatc tttcaactca
     9781 tccgacggcg cggcgaacac cctgtgatgc agttcgttct cgtccgccca ccaatcgacg
     9841 aagatgaaat tcgcgccgcg cccgtcgtgc acgccgacgt agccgacgcc gtagttcccg
     9901 ccctcatgcg ccgttggtag agactcgcgc gccagacgcc tcgccgtctc gatcaactcc
     9961 tcgcgcgcac gcacgcgccc gtaactgatg ccgtaaactt tcagccgcca gcccgcctgt
    10021 tgccactctt cgagaaaacg aatcgggcgc ggctcgtaag gctctcgcaa taacatgaag
    10081 tcgtgcgccg tgaatcgtca atcgtgaata gaggaaaact gtcttagtct atttacgctc
    10141 tactgattcg gagctactga atttcgctct actcgaactt cacttcgtca atctgcgcgc
    10201 gctgcaactt gccgctgtag tcaacgtaga cggacttcca ttcggtgaag atgtcgagca
    10261 cttgcgtgcc cgcttcgcgg tggccgttgc cggtggcctt cgtgccgccg aagggcaggt
    10321 ggacttccgc gccgatggtc gaagagttga cgtagaagat gcccgtgtac atgtcgttca
    10381 tcgcgtggaa ggcgcggttc acgtcctgcg tgtagatggc cgccgacagg ccgtagcgca
    10441 cgccgttcgc gatctcgacg gcctcttcca gattgtttgc cgggatgacg gcggtgactg
    10501 gaccgaaaat ctcttcctgc gcgatgcgca tgccgggcgc gacatccgtg aacacggtcg
    10561 gctcgatgaa atagccgttc gcatgtgcgc ccgtgtccag acggctgcct cccgtcgcca
    10621 gtcgcgcgcc gtcttctctc tcgccgactt cgatgtagcc cataatctta tgcaccgccg
    10681 cggcgttgat cacaggcccc atatccgtgc tcgcgtccgc accgttgccg acgcgcaaac
    10741 tttcggcgcg ctcgacaagc ttctgcgtaa acttcttata gaccttcttc tgcacgacga
    10801 tgcgcgacga agccgtgcag cgttgccccg atgtgccgaa cgcgccccag accgcgcctt
    10861 cgacggcgag gtcaaggtct gcgtcgtcca tcacgataat gacgtttttg ccgccgagtt
    10921 cgagcgagca gatggcgttg cggtcagcgc agaggccagc gatgagtcgt ccggtttcgg
    10981 tcgaacccgt aaacgagatc aggcgcacgt cgcggtgctc tgtgagcggt ttgcccgcct
    11041 ccgcgccgta gcccgtcacg agattgacga cgccgcgcgg cacgcccgct tcttcgcacg
    11101 ctttgacgag attgtaagtt gagagcggcg tgtcttcggc gggtttgatg acgaccgtgt
    11161 tgccgcagac gagcgcgggg atcagtttcc acgaagggat cgccatcggg aaattccacg
    11221 gcgtgatcaa accgcagatg ccgacgggct ggcgcacgca catcgcaaac ttgtcgggca
    11281 tctcggcggg cgtcgtgtag ccgtgaagcc gtcggccttc gcccgccgtg tagaaggtgc
    11341 aatcaatcgc ctcctgcacg tcgccgcccg tctccttcaa gaccttgccc atctcgcgcg
    11401 tcatgtccgc cgcgtggcgg tctttgtcgc ggatgaggat gttgccgagg cggaacaata
    11461 tttcagcgcg acgcggcgcg ggcatccgcc gccagccgtc cgccgcactc ttcgccgccg
    11521 cgaccgcgcg ctccacatct tccgcgttcg agacgggaaa gcgaccgatc acgtcgcgcg
    11581 tgtccgccgg gttgatgttc tcgaaatact cgccgctcgc cgccggaagc cattcgcccg
    11641 cgatgtagtt gctgtaagtt ttgacggact ggcgcgcgcc ctgcttcccg ttgccgttgc
    11701 tgctgctgcg acccgcgctc ttcttcgtcg tccggcttaa cgcttttttt gcctgtgcca
    11761 taagaaagtg atgagtgatg agtgatgggt gctgagtaag agcaattgtt tttcactcat
    11821 tactcagcac ccatcaccca tcacttttta ctggtaagtg aattggataa tggtttgcgt
    11881 gatgagatta cggcgatcaa ggaacgtgac gttcatcacg acggcatcgc cgactttgag
    11941 gccgctgatg acgcgctcga actcggccag cgtgtttacc ggaatccggt tgacgcgctg
    12001 gatgaccatg ttctccacga tctgcgcttc ataggccagc ccgctctggt ctacgtcgcg
    12061 gacgagcagg ccgcgcacgc ctttcaggtt tcgctcgttg gcgagttgcg gcgtcagttc
    12121 gctcaaggtg aggccgaggg tcgggcgttc gccttccggc ttggccgggg tcgctttgga
    12181 cggagaaggc gtcggcgtcg gcagggcatc ggacgctccg cgcggcggac gctcgccgac
    12241 cgagacgccc acggtgcgcc gttcgagttg gccgttcagg tcgcgcaggt aagtgagttg
    12301 caccgtctcg ccgacgggcg tgctcgccac cttgttgatc aagtcctgcg aattggcgat
    12361 gggcttaccg ttgaactcga cgatgatgtc gttgacctgc acgcccgccg cccgcgccgg
    12421 accttcgcgc acgtccgtga tgatcgcgcc cttcgcatcc ttcaatccgt agacgcgcgc
    12481 aaattccgcc ttcaccgaat cgagcagcac gccgagataa ccgcgcttga cgcgtccgcc
    12541 atcgacgatt tgacgataca cgaagctcgc ttcgttcgac ggcagggcga agccgatgcc
    12601 gttgtagtcg ccggtcgagg tggcgatctg cgaattgatg ccgatcactt cgccgcgcat
    12661 gttgacgagc ggcccgccgg agttgccgcg attgatggcg gcatcggtct gcaaaaatct
    12721 ctggaagctg gtggagacgg gcgtggtgcg ctcgcgcgtc gagatgatcc cggcggtgac
    12781 ggtttgatcg aggccgaagg gcgagccgac ggctaagacc caatcgccga cctgcgcttc
    12841 gtccgagttg ccgagtttga ccgtcggcag ttcgcgcgac gtttcgactt tgatcaccgc
    12901 aaggtctgtc tcgtcgtctt caccgacgac ccgaccgcgc atgagttcgc ccgattgcag
    12961 gcgcacggtg atgcgcgttg cgccttcgac gacgtgatgg ttcgtcagga tgtagccttt
    13021 cgagtccacg atgaagccgc tcccgacgcc gcgcgagggg cgttgcggca tctggcggcg
    13081 aaacatctcc atcagaggat tatcgccgcc gccgtccttg tcgtccgaat cgtcgtcgct
    13141 gtcattgttg ctgttgccgt taccgttgcc gctgcccgtg ccgccgcgct ccgccacctg
    13201 cggcgcggag acagtgtcga tgttgacgac cgccggttcg acgcgctgtg cgatctctgc
    13261 gaaggacgcg gagagggctt cgggcgcgcg tgcgttctgc gcggcgcgtt gcgtctcgtt
    13321 ctgcgcgacg gcgggacgcc ccgccagcat cgccccgacg ccgacgccga ccaccagaca
    13381 cgcgagagcc agtgccgcga tccacacacg caggcgacgc aggatagcgc gctccgcctc
    13441 attcgtttgg ccttggggaa gttggtacat agaactttta ccgcctttta ccgctcataa
    13501 aaaacacgac gatagttgaa cgtcatgcgc tcacacgact gtcaacgaat gaccgccgcg
    13561 cccttgtgtt gtccgcgaag ccctgattcg cgcctctgtg cacgaagcga agtttgtcga
    13621 gtataacgaa tcgccggacg cgccgcaaag caacagccgt gcacccacgc gcctgcggct
    13681 gaaacggcga agaaacgccg ctcaggcagc ttcggcttcg cgcgcctttt cgttcgccct
    13741 gcgctctctt ctctcactcc aacgcatctc ggagatgatg atcccggcga acaccaacgc
    13801 cgcgccgacg aagccgcgcg tgcccatcca ttcgcccgcg ccgatgacgg ccacggcgaa
    13861 gagtgtggcg aagacgggtt cgaggctgaa gatgatggcg gcgtgcgtgg cggtggtgcg
    13921 cgcctgtccc cacgtccagc agaggaacga aacgactgtg gcgaccgtcg ccagatagac
    13981 gagttgccac acgacgcgcc cgctccagac gagcggcgca gtctcgcgtg cgaacgcctg
    14041 cggcatggcg tccgcgcccg cgataaaatt ccatgcttgt aagcctgccc atatgaacgt
    14101 gaagagacag gcggtggagg cgatctgcaa gacggtcaac tgtcgcacgt cgtagcggcg
    14161 tgcatagacg ctcatcagcg tgatgtgcgt cgcaaacagc gcggtcgcag caagcgtcag
    14221 cagatcgccg aagttgatcg tgccgtccgc cccttgcggc gcgagtatga gcaggccgcc
    14281 gagcgaggca aacgcgacgc cgatcaaatt ctcgacgctc ggcctcgcgc gcaggatcaa
    14341 ataggcgacg aagggcacga gcggcgtggt gagtccggtg acgaaagccg actttgaggc
    14401 ggtggtataa acctgccccg ccgcctgcaa cccgaagccg aagcccatca acacgccgag
    14461 cgtcgcgccc gcgcgccact cttccctgcg cgcacgcgcc agttgctgcg ggaacatcac
    14521 ggcgagcagc aacgccgccg ccgcgaagcg cacgaacatg taagccatcg gcggccagac
    14581 cgtgaggatg tccttcgcca tgaagaaggt cgaaccccag atgagcgtcg tcaacatcaa
    14641 cgcgccgtct gccgccagat tggatttcag cctcatatct caactacagt gacgagtgac
    14701 gggtgacagg tgacgagata agaaagtgaa tcgtcaatcg tcaatcgtga atagaagaag
    14761 actgactttc tctattcacg attcacgatt cacggctttt tctcatcact cattactcat
    14821 cactcatcac tcatcacttt tctatctcgt cactcgtcac taccttcaag gtttgagcag
    14881 aacttttaag acgccgtttt cggttgcgcg ttgcatggcg cggaggccgt ctgcgagcgc
    14941 gaactcttcg gtgatgaggc ttgcaacgtc tacggcgtcg cgtctgagta gttcgagcgc
    15001 gggggcgaag cgtccgcagc gcgagccgac gatgctgatc tcttcgacga cgatgcgcgc
    15061 ggcgtcgatc tcggtcgcgc cgtgaaaagt tgacttcaag acgagcgcgc cgcgcgggcg
    15121 cgtgagacgc agggcgagcg cgaacccgct cgccgcgccc gacgcttcga caaccacatc
    15181 gaacgcctgc gccgtcaggt cgtcgcccga cgcttcgacg agcgcggttt cgacgccgcg
    15241 tgcgcgcgcg atgtcgagtt tcttttcatg cctgccgagg agcgtgacgg aagccccggc
    15301 gcactgtgcc ttgatggcct gcgcgcagag gagtccgagt ttgccgtcgc cgacgacggc
    15361 cacgcgcatg ccttcagtaa tctcgacgcg ctccgtgatg ccgcacgccg ccgcgagcgg
    15421 ttcggcgaag acggctcgcg catcctcgat ctcatccggc acgggcaata aatttaccgg
    15481 gggcagccgc aaaaattctg cgaacgcgcc gtcgcgctcc ttgatgccga gcaccgtgcg
    15541 ccgtggacaa tggcgcgcgt cgccccccac gcacaactcg cacagaccgc aacccgcgtt
    15601 gatctcgccg accacgcgct gcccttcgag ttgcggcgcg cccggcgcgc tctccaccac
    15661 gccgacgaac tcatggccga gcgtccccgc gaatcccgcg tacccgcgca cgatctccag
    15721 atcggtgttg cacacgcccg ccagcgtcac gcgcaccaac gcctcgcccg cctcgcctcg
    15781 cggcgtgccg acttccgcca gccccaaacg cccattctcg aatcgcagtg ctttcataaa
    15841 tgagatcgta aaaaccgtga atcgtcaatc gtgaatagag aaaacctgtc ttagtctatt
    15901 tacgattgac gatttacggt tttcaaaact ccttcgtcct cgtataagcg ttcgcgttgg
    15961 tgagttcgac cgtctgctcc cgaccggcga cgttgacggg gagccgccag gtgatctcct
    16021 gcgcgcctcc ctgcgtggcg tgtatccacc agcgaccggg cggcacatag acgatggttt
    16081 ggccttgcgc gtcgagtttg gtggtgatgg gctggcgcgc gcccatgcgc tgcacgatga
    16141 agtcttcgaa gcgcatgccg gggcgtcgct cgtcgaagaa ctcgcgctgc gccgccgcca
    16201 cgtcaatcga gtagagttgg acggcagtga cggagtttat ggcggcggcc ttggcgctat
    16261 ccatgcccgt gtgcggcggc aggatgatgc gcagcagcgt gtcgcgcgcc tgccggtcgg
    16321 tgccgagtcc ggcggcgggc gtcgtgtcgt cgtgcgggag gcggcgatag atgagccagc
    16381 ccgcgccgac gagcgcgacg acgagcgcga ggcgcagcac ggcgacgagc agttgatgtg
    16441 cggggctggt gttggtcttc actcgaaact atatccggct gaaacgagta gggcgcgcgg
    16501 tcaggccatg aatccctttt cgcgcagcgc gctctccaac tcgcggttgg tcggaaccga
    16561 gagcacctgc ccggcgatgt cgagcgtggg tttgcgctcc ttgccctcgt tgtgctgctt
    16621 cacccactcc gaagccttcg cgtcctcttc cacgttgata taggcgtgcg ccacgccgag
    16681 ttccttcaag tgcttcagag tatcctgcgt atcgtgacac caatccgccc cgtaaacttt
    16741 gacttccatt tttccacctg tccttcgaat tattttccgg caatgataac aaggaaacgt
    16801 tgaagtagga acgcggaacg atgaatgaac cgcaatccgc ttccgttcat cgctccgcgt
    16861 tcatccttca tcgttcccct gtcgagtgtt acaatgcgtg ttcaacttcg aaattttcta
    16921 cagcaaatat ttccagtgac gacgaacggg acagataaat ttcgcgacga gtgcggcgtc
    16981 ttcggcatct acggccacac ggaggccgcg cgcctcgcct acctcggact ctacgcgctg
    17041 caacatcgcg ggcaggaatc ctgcggcatc gtcgcttccg acggcagaga gttgagaagc
    17101 gagcgtgcga tgggactcgt ctccgacgtg ttcgacgcag aacgtctcaa ccgcctgccc
    17161 ggcgcgtcgg ccatcggcca cgtccgctac tcgaccgccg gggaagtctc catacgcgag
    17221 gcgcagccct tcctcgtcgc gtgccagcac gggcaagtcg ccatctgcca caacggcaat
    17281 ttgcccttcg ccaccgagga gcgcaaacgt ctggaacgcg cgggcgcgat cttctcttcc
    17341 acgtccgaca ccgaagtgat ccttcacggc atcgcgcgct cgcaagccgc aggcatcaac
    17401 gaggccatcc ccgaagtctt gcgcgagacg gaaggcgcgt tctcgatgct ctttctcacg
    17461 cctgactctc tcgtcgccat ccgcgacccg cgcggcttcc gcccgctcgc gctgggcaga
    17521 ctcggcgata acacctgggt cgtcgcctct gaaacctgcg ccttcgatct catcgaagcg
    17581 cgctacgtgc gcgacgtgga acccggcgag atgatcatca tcgacaaggg cggcctgcat
    17641 tcctcttacc cgttgccccc gcgcgagcat tcgatgtgca tcttcgagca cgtctatttc
    17701 gcgcgccccg actctctgat ctacggacat tcggttaacg aatcgcgcca caagatgggc
    17761 aagcggctcg ccgtcgaaca accgaccgag gcggacatcg tggtgcccgt gcccgattcc
    17821 ggcgtcgccg ccgccatcgg ctacgcggcg caaagcggca tcagttatcg tcaggggttg
    17881 gtgcgcaatc attacgtcca tcgcaccttc attgagccga agcaatccat acgctccttc
    17941 ggcgtgcgca tcaagttgaa cccggtgcgc catctcatcg aaggccgccg cgtcgtgctc
    18001 atcgacgact ccatcgtgcg cggcacgacc tcgaagaaga tcgtccgcat ggtgcgggaa
    18061 gccggggcga cgcaagtaca catgaggatc agttgcccgc ccaccatcag cccctgcttc
    18121 tacggcgtag acacgccgaa ccgcgacgat ctcatcgccg cgcagatgtc cgtcgcagag
    18181 atacgcgaat tcatcggcgc agactcgctc ggctacctct cgctcgaagg catgctcgcc
    18241 gccaccggca tctctcccgc cgcctcctgc gtcgcctgct ggacgggcaa ctatcccacg
    18301 cagatcacgc gcgaggccga aacccaatgg gcgcgcgaac acgaacccgt cccgtccgac
    18361 taaggaagcg cctgcaagtt tttataaatg cgcattatta cgcgtaagcg tctaaatgat
    18421 tttgctgttc gatatcccga aacgcgaacc ggtctggcgc actggtacca actaatgaag
    18481 cagagcagtt ttgcttcgtt tgctgaattg cgcgcgattt ttccggcggc ggatcaggtg
    18541 ggaaagttga ccgtcttcaa tatcggtggg aataaggcgc gactcgttgc agcgattcat
    18601 tacaatcgtc gtagaatata cgtccgggcg gtgctcacac atcaagagta tgatcaaaac
    18661 cgctggaagg aataagcaat gcagaatcgt gagcttgata aaactttaag cgcatggcct
    18721 ttggtgtcac agaaaattta tgttccgcat accgaagaac aatatgagcg gatagtcgct
    18781 ttgcttgatc agttaattga tgaagttggt gaagacgaaa cgcaccctta ctcgtcgtta
    18841 atggaactgc tcggcgttct catcgaacat tacgagacag aacatgtcac cgaattaaca
    18901 atcgaccaat aacagtgcgg atatttcaag aaggagaata ggtggcacag agtagaagca
    18961 tacgcggttg cgggtgcgtg ctggtggtgg cggcgatcat cgtgggcgtc gcgctgttct
    19021 cgtatttcag gttctatcgc cccgcgccgg ggccgcagct tccgccgccg tcgggcgacg
    19081 aactctcgat tcacgtgctc gacgtggggc agggcgacgc catcctgatc gttgcgccgg
    19141 gaggcaagac cgcgctcgtg gatgcgagcg tgccggggag cgggaagaag attctcgccg
    19201 cgatggggcg ttacggcgtt gaccacatcg atctcctcat ctcgacgcac gcgcacgcgg
    19261 atcatatcgg cggcgcggac gaagtgatca aggggacgaa agtttacaaa gtgctcgaca
    19321 gcaaagtgcc gaacacgacg aagaactacg aggatttcct cgatgcgatt gaagccaaca
    19381 aggttgagtt tatcggggcg cagcccggcc agacgttcga tctcggaagc ggcgcgatca
    19441 tctccgtgct cgcgcccata cagccgctct tcacgaaaga ccaactgcgc tccggcggca
    19501 acgaaccgaa cgccaactcc gtcgtcacac gtctcgacta cggcgagttc tccatcctct
    19561 taacgggcga cgcagaggcg cagaccgaac ggcgtctgat ggagcagggc gcgcgcctcg
    19621 aagcgaaagt cttgaaggtc ggccaccacg gctcgaagta tgcgagttcg gaagatttcc
    19681 tgaacgcggc caggttcaaa gacgcgttga tctcctgcgg cgcagataat cgctacggcc
    19741 atccgagcca ggaagtgtta gaccgtctca aggccgccgg tgtcaaaatc tatcgcacag
    19801 acctgcaagg cgagatcacg atcaagacgc gtggccggga ggactatcag atcacgaccg
    19861 aacgccaggc cgatgccacg gagatcgcga cggggcgcaa ggcgcaaaaa gacgactcgg
    19921 ccaagtcggg cttcatacag tacggcgact tcggacccgc gccgaaggct tctcccaaag
    19981 ccggagcggc caacagcaac cgggcgaaca gcaaccgggc gaacagcaac agcaggtgac
    20041 gttgatgaca gagcgcacag acctttcgcc gccaggacgc gccgacgaaa ttgaggttcg
    20101 cgccgtcatc gaccgcatcg aggacaatga tgtcgccgtg ctcatgctgg aagacgaagc
    20161 ggagtcgcaa ctcgacgtgc cgcgcggggc tttgccggag gatgcgaact cggacggcga
    20221 tcacctgatt ctcaagttcg acgttgacgc ggagagcggc gagcgcacgt cgctcaagtc
    20281 catcaaggcc gcgcccgccg cacgcgccgc cgccgcagac cggatcaaaa agatgcagga
    20341 gcggctcacg cgcatgagcg gcgcggacga taaaaaagat ttcaagctat agaattcagg
    20401 agtcagaatt cagaatgcaa gcgtcagact aaattgctct ccatcggtca gccttctgtt
    20461 ttctgaattc tgtcttctga cttcgcgctc tcatttctta tgaccaaacc catcacttac
    20521 agcgacgcgg gcgtctcgat tgcggcggcg aacgcggcgg tcgataagat caaggagatg
    20581 gcgcgctcca ctttcaacgc gcgcacgctc tccgagatcg gcagcttcgg cgggatgttc
    20641 gacgcggcgt ttccctcgat gagcgcgccc gtgctcgtcg cttcggcgga cggcgtcggg
    20701 acgaaactca aggtcgcctt cgagacgaac acgcacaaca cggtcggtcg cgatctcgtc
    20761 aaccactgcg tcaacgacat tctcgtgcag ggcgcgcgcc cgctcttctt tctcgactac
    20821 gtggcgacgg gcgtgctctc gcccgaagtg atcgcggcgg tggtggaagg catgtcgcgc
    20881 gggtgtcgtg agaacaactg cgtgctgctc ggcggcgaga cagccgagat gcccggcttc
    20941 tacgcggcgg gagaatacga cgtggcgggc ttcatcgtcg gcatcgtaga ccgcgagcgc
    21001 gtgattgacg gcaagcgcat ccgtgcgggc gacgtgctgc tcgcgttgcc ttccgtcggc
    21061 ctccacacga acggctactc gctcgcgcgc aaactctttt ttgaggtggc gggctacgac
    21121 gcgcgcacgc gcctcgcaga actcgacatg acggttggcg aagcgttgct cgcgccgcac
    21181 ctcagttatc tgcccgcgct cgaaggtctg ctcgacacgg gcgcgctcaa aggtctggcg
    21241 cacatcacgg gcggcggact cattgacaac attccgcgca tcctgccgga aggcacagcc
    21301 gtcgagatca aacgcggctc gtggcctgtg cttcccgtct tcacgctcat gcagcgcatc
    21361 ggcaacgtct cggagacgga aatgcaccgc accttcaaca tgggcgtcgg catggtgatc
    21421 atctgcgcgg agacagacgc cgtcaccatc cgttcacacg tcgaagcgcg cggcgagcga
    21481 atcttcgaga tcgggcgcgt cgtcgcaggc gcacgc
//
LOCUS       DASWNS010000618        26063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_49751, whole genome shotgun sequence.
ACCESSION   DASWNS010000618 DASWNS010000000
VERSION     DASWNS010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 26063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26063
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49751"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(619..1740)
                     /locus_tag="VGB76_21280"
     CDS             complement(619..1740)
                     /locus_tag="VGB76_21280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0101472.1"
                     /translation="MRFIFSFLRWFKCPAGFRVLRRVGVSTCALALLVAASAWGQLAE
                     VAFAQGQQKTEKRSPSASQEIDDDEVIKVSTTEVLLPVTVRDAAGRLVTTLERKDFQV
                     WENGREQPLSDLRVRRVPVDVMLMVDSSSSVTSNLEDFRRAAEEFAARLAPEDRVSLL
                     KFDDRVELLQDWTRSRVQLRRSLGRITPGMFTRFHDALYLAAREAFRDAPQRRHAVVV
                     LTDGIDSGRGHASLEMALGALLEAGASVYCISNTEIERARKSAELDTLQSTPPSTRRF
                     NELRIGDLRESLRVLDASERNLSNLTAATGGRLFKPHSFTALEQIYAEVAEELRSQYA
                     LYYMPLDKTRDGKFRRVQVKTSDPALRLTARLGYFAPRS"
     gene            complement(1803..2639)
                     /locus_tag="VGB76_21285"
     CDS             complement(1803..2639)
                     /locus_tag="VGB76_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101473.1"
                     /translation="MARHGARLLLCVAAAGYGAQETRGQLSSARTATAASATQTDEAT
                     PLTNSSIVKLVRAKFSEKTVIAIIRVRPVRFDLSPDRLIELKKHGVGERIILTMLARS
                     EGDAFAARDDAAGDAFEDDPFFDGTRPSGRAKDAGKPSGVGSDPNETSIFGSSGGVRG
                     RSRSSIGGGAAEGDTQTTGSASVRILRPPAEAGGAEPKLERTPTLTNDSVIELVEAGF
                     SEGTIIRRIESSPVEFDFAPAKLSELRRRRVGEPVINAMRAAMNEEGAASNGANDFNA
                     KP"
     gene            complement(2698..3717)
                     /locus_tag="VGB76_21290"
     CDS             complement(2698..3717)
                     /locus_tag="VGB76_21290"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0101474.1"
                     /translation="MLKEEISRRRARSLFYGSQVARLKVAAFAFLFVLGLPAIAFADR
                     LLLVDGTTIEVDEAWDDAEGVWYRRGGMTNFVERARVKTIERKGTEKEASKARQVAKL
                     SDVNAAGDETAKSSQVATVQRPLWIYLVGGARFEVDEANETAEGVWYKRGNLSIFLER
                     ARIERIERETDELKDETTATSTTAQAAKRERRWTTGRPHLDALIRQNGARYGVDPYLI
                     FCVMEQESHFNAGAVSPVGARGLMQLMPGTAARFGVRRVHDPAQNIAGGTRFLKQLLG
                     RFNGRVELVLASYNAGEGAVAKYGNRVPPYRETRNYVRRISGRYGRAAHAVSERRTTT
                     ADAGN"
     gene            complement(3736..5100)
                     /locus_tag="VGB76_21295"
     CDS             complement(3736..5100)
                     /locus_tag="VGB76_21295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101475.1"
                     /translation="MTQTNILTRMEVESMGPPVPEELAETDVPESFLCDLALKIVAAL
                     PEPSTANVTERLRLPRALTEEILQQLYREKLIEVKLQSAMGATRYGMLDKGWERLARA
                     RAFCGYDGPAPVSLRDYAHMMRLQAVPARHATMDSVRAAFRDLILPDSLMQTVGCVVN
                     SRRSLFLSGPPGTGKTAVAERINASLPGAIWIPYAIEIDGQVIRVFDAHNHRPAANDA
                     TPAEYDRRWVCIDRPLVIVGGELTLENADLTWSEAARFYEAPFQMKSNGGTLVIDDFG
                     RQRVAPADLLNRWIVPLERRVDYLTLHTGKKIEVPFEQLVVFATNLEDKDLVDEAFLR
                     RMGYRARVEPPTPAAYIEIFKRAAHTRGMRVEQVCLDYILNNYMISHRQMKACEPRDL
                     LDRVLDICLFEGQPIELTPKVIDSAWRNYFGTSHGFAQETESQRRGAPGEVRSVGDLV
                     KVDA"
     gene            complement(5140..6129)
                     /locus_tag="VGB76_21300"
     CDS             complement(5140..6129)
                     /locus_tag="VGB76_21300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101476.1"
                     /translation="MRHRTINAFIIALVLTISVTTFWTAGTTERDGDDAANVVASEGS
                     PTGATEDAAAGSEEKQKKKGNRFARFLKAPFKAAAKLFGGDDDKVRRLSEKDVAKFES
                     APLMRVEDSRTASAESHTDEGGTARDYLARGRTRLDEGRLNEAIRELSRAVSLDARLS
                     EAHNLLAVAYDRKGLPERARESFARALDHEPDAQTLNNLGYSLYLSGNYRAAISRLKR
                     AAKLAPADERIWNNLALAQCRLGKYDDAYKSFARSGGEYQGRLNVAAMLERAGREDQA
                     IEHYEAARRVQPQSEFVLRRLVELYARNGQGERAEQTRRALDGRANEQVAGKE"
     gene            complement(6144..7097)
                     /locus_tag="VGB76_21305"
     CDS             complement(6144..7097)
                     /locus_tag="VGB76_21305"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0101477.1"
                     /translation="MILIITLSTFAFITLGVLGIYWLMFRPASAATERLRRMGETGGG
                     GAVLRAGGVDLSANAASDDGMTALAERMAKPLHRLAPPSAAEAKKLQKQLMQAGYRSP
                     NAPIVYRAIQISTLIGYPAIVALACALTARPLSSAMMWILGAFIKGFFLPRYALRKMV
                     KSRQQRVRWGLADALDLMVVSIEAGLGLNAAIVKVGEELREVHPEISEEFELTNLEIR
                     VGRDRDEALRNLAERTGVDDLRSLVAMLIQADRFGTSISRAVRVYADSLRTKRRQRAE
                     QAAQKAAVKLLFPLACFLFPTLFIAILGPAALNLIDSFAKM"
     gene            complement(7247..8209)
                     /locus_tag="VGB76_21310"
     CDS             complement(7247..8209)
                     /locus_tag="VGB76_21310"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0101478.1"
                     /translation="MIPFFVFAFCLFLTFAGYLLATRGSEKKRVRLQQRLSEALLHSA
                     HDADVEVQLARQELMSEIPLLDRMLVRVQVATKLKRMLDQADLHITVTRLFMFSLMAA
                     MLAGLAASMLTISTILIVMAGAAAAVVPFVHVVWARRKRLNKFLEDLPDTLELMSRGL
                     SAGHGFAEALHMVATEMPDPIATEFRKTYEEQNLGLSLKLALDNLAARVPLLDLKLCI
                     TAILIQRETGGNLAEVLEKVAHTIRERFRIMEDLKTLTTSSRMSAWILCGLPIFVALA
                     VTVMNPEYMSVMWNDPRGHKLMAVAIGLQITGMLVIRKILNIKI"
     gene            complement(8345..9694)
                     /locus_tag="VGB76_21315"
     CDS             complement(8345..9694)
                     /locus_tag="VGB76_21315"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY0101479.1"
                     /translation="MSLRERLVRETNPALMPVARTGDEAATGGQHTFQEMKSRLHRAI
                     INRMDLTKLSQLDADQLRAEVSRLAEGLLAIENMPLSTSDRERLVEEVRHELFGLGPL
                     EPLLADPTISDILVNSPHNIYIERRGKLERTEVAFKDDEHLMRVIERIVSSVGRRIDE
                     SSPMVDARLSDGSRVNAIIPPLSLDGPVLSIRRFGAEPLRMAKLIEIGALTKDIADMF
                     EMCVNARLNILISGGTGAGKTTLLNALSAYIPEEQRIVTIEDSAELQLQQPHVVRLET
                     RPPNIEGRGEVTQRDLVKNALRMRPDRIVIGEVRGGEAIDMLQAMNTGHDGSLTTVHA
                     NTPRDALARLETMIQMTGMKLSERAMRQQIASALNLVIQAARLSDGTRRVVSISEITG
                     MEGDTITMQEIFQFERKGIDKDGHVVGRFRPTGVRPRFAERLKVYGMQLPRVFFEEN"
     gene            complement(9767..11011)
                     /locus_tag="VGB76_21320"
     CDS             complement(9767..11011)
                     /locus_tag="VGB76_21320"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0101480.1"
                     /translation="MNQLSFVILSTGLESLKELRGALAANEQTRLLAGGDDAEQLFPE
                     VVRLRPNAAIITFGAQPETALKFVERLAAECPQTAIICASRDASPDLILRSYRAGARD
                     FLRLPVIAEEFQTVIERTAEFCSTQVAAPKRRGRAVAVFSSKGGCGTSFIATNLAAAL
                     DAPTVLVDLNLQAGDLSLFLGLEPKFSIADLVENRARIDDSLLKSYVMPHTSNLSLLA
                     APREADLADDIEPEHIFEVLELLRERFDYVVIDPQHTFDAITLSALDQADEVVLVLTL
                     DIPAIRSTQRALALFDRLGYPRHKVRIVVNRWSKQIDLDLRQVERFLGERVTGYVQSD
                     YNTAVNSINLGEPLVKSNPTSRIAQEIRHIAASITGASVESEIKEPRKGVWGAIFGRP
                     VGAISNVGLRSRLNKDKATAAI"
     gene            complement(11079..11534)
                     /locus_tag="VGB76_21325"
     CDS             complement(11079..11534)
                     /locus_tag="VGB76_21325"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY0101481.1"
                     /translation="MRAKIFRRSDERGAALLEFAIGATLFLSASFAVIEVGRLLWVHN
                     SLVDATRRAARYAVNQGMSTGAQTQAKNMAVYGNTGGTGQPLVQDLEPTHVKLTYQNF
                     ELGGGTVTAEIENYDFKFVLPLVNKTMRLPAYKTSLTGENAGYVPTVIP"
     gene            complement(11563..12051)
                     /locus_tag="VGB76_21330"
     CDS             complement(11563..12051)
                     /locus_tag="VGB76_21330"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY0101482.1"
                     /translation="MRSQAELTQRFTRACGRRLRALRDCERGSQLVELAIIMPLLLVL
                     LGATAEFGRFFYTYTTLLKGTRAASRYLVSQPVGKADSAARNLLVYGNTAGTGTPVAS
                     GLTTANVVITPNKAGAKTQTVEIQNYTYVPIFDLGKLTRSSTLSLSVNVGAKSTMRQL
                     VN"
     gene            complement(12065..13225)
                     /locus_tag="VGB76_21335"
     CDS             complement(12065..13225)
                     /locus_tag="VGB76_21335"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY0101483.1"
                     /translation="MKRTSQKESLRERRGERGSVLAISTFGMLAFLLATGLCIDISHF
                     YLVRGELQNAADAAALAGASALNSDDGGITTAANYAVQSLNSYEFNKKGVTIPRENVL
                     FAKNLNGPYVSEATAKGQAKDIRFVKVMIPPAAVATSFAAPVLGASRNVAADATGGMS
                     VSLNRFCDYIPVTAIDADDVYFVKGQTYTIRRPPGGAVSPGNYQILAIDGPGASDDRI
                     GLGKGVRNCVGAGEYVKTKPGVSAGAVRQGINTRFGEYGGGLDPSEYPPDENVQEHIN
                     YQQYLDAQQAIDADKTPAANTFEAPTRGVPERGRRVVLIPIIRIAEFEGGRDSVKIDR
                     FAAFFLQTKVDGGNGGDIKAEYIGIRTVLGRGGYDPNGGAVSPELTIPVLYR"
     gene            complement(13295..14938)
                     /locus_tag="VGB76_21340"
     CDS             complement(13295..14938)
                     /locus_tag="VGB76_21340"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEY0101484.1"
                     /translation="MQTRRLIPFIIAMLCASFIFSVTGAAHAQETSVSASFANPQKES
                     VAINVLVGQSRVINFDRKIGRFSVSNPEVAEAVLVAPDQVLVNGKAFGQVNFIAWEKE
                     SGKFVVFDVFVRANLSLIDSQIRALFPNDDIRLSQANGSVVISGSVAQAQTSTQVEQV
                     VKAAGFQTVNMLTSPAKNAMQVQLSVRVAEVSRTKLRELGSSWASSGNGTSVSATNSL
                     ASLFETANLFISNTGVGSAAAIRALQTSGAIRSLAEPNVIAMSGQQASFLAGGEFPIP
                     VIQSDGQRVNVTVVFKEYGVRLNFKPTIIDEDHIQLELEPEVSTIDFQNGVKFQGYLI
                     PALKTRRAKTGIELRDGQSFALAGLLDNSESSTLSKVPGLGNIPILGALFKSKSFEKR
                     ETELVFFCTATLVKPVNRDDLPATRGLDGLKKGSPLGVEPQGEGIKGASGYSTGTDGA
                     KATSATDANTATQTVAPAVSPLASPATTTPAKDAVKEEKDASKETAKAALKEQAANTA
                     QEAVLPPAGNAAHAVLSSYFGATQNAPLLSSTAKVSAKQ"
     gene            complement(15053..16003)
                     /gene="cpaB"
                     /locus_tag="VGB76_21345"
     CDS             complement(15053..16003)
                     /gene="cpaB"
                     /locus_tag="VGB76_21345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY0101485.1"
                     /translation="MRNKRLVFVMCGAVVFGLIAAFSVSRYLSGTQAYAKNLNTVVVA
                     KVDIQPGEKIIAEQLQSVQFPSTAMPEGTFQSMEKLVGRVAVVQIAAREPVTDFKLAP
                     EGSAGGLSAVIPEGYRAMTVKVDDVVGISGFVMPGALVDVVVVINPVEQNGQQNPISK
                     IVLQNIKVLANGQNIDRPKNDREPESVKAVTLQVLPEQAEKLALASTEGKLQLVMRNS
                     VDQGDEQTLGVDKRVLLGGERAKPVPEAGSLKSEQPQQTQQPAPVMRRSYPRADNSRS
                     SNSAPAPQAATPPAAPAPAPPRSSIEVIEGAKKRNVDFPQ"
     gene            complement(16092..16619)
                     /locus_tag="VGB76_21350"
     CDS             complement(16092..16619)
                     /locus_tag="VGB76_21350"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY0101486.1"
                     /translation="MSGTLTIVSSALLVPLAVVITYYDARYRRIPNAFVLATLVAGLS
                     VNTIINGTHGLLASLGGCALAFALMFMLHIFGAMGAGDVKLFAAIGSLLGASLVVPTF
                     LVVVLTGGALAVVTMFRAGTVRETLWRVVQIFVGFLPGWEMPRFSVPAERRYTIPYGV
                     AITLGSLISLALFRA"
     gene            complement(16772..16936)
                     /locus_tag="VGB76_21355"
     CDS             complement(16772..16936)
                     /locus_tag="VGB76_21355"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0101487.1"
                     /translation="MVKKFFQDETGLELSEYAVAAALVALAVITAFTTLGTNIGTKIT
                     NLATSIASTP"
     gene            complement(16978..17139)
                     /locus_tag="VGB76_21360"
     CDS             complement(16978..17139)
                     /locus_tag="VGB76_21360"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0101488.1"
                     /translation="MIKKFMRDETGLELSEYAVAAALVALAVITAFTTLGGNIGTKIN
                     NLATKIGTP"
     gene            complement(17185..17343)
                     /locus_tag="VGB76_21365"
     CDS             complement(17185..17343)
                     /locus_tag="VGB76_21365"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY0101489.1"
                     /translation="MIKKFLRDETGLELSEYAVAAALVALAVITAFTTLGTNIGAKIN
                     NLATKIAP"
     gene            complement(17428..17637)
                     /locus_tag="VGB76_21370"
     CDS             complement(17428..17637)
                     /locus_tag="VGB76_21370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101490.1"
                     /translation="MMKTLLNKTFLAVAALLLVLPFVAVALLAGERAVAVVAGGANGN
                     WLLVALAIGGVLVSSWHGLMKHPVL"
     gene            complement(18069..19622)
                     /locus_tag="VGB76_21375"
     CDS             complement(18069..19622)
                     /locus_tag="VGB76_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101491.1"
                     /translation="MREFEKAGDAAWPVVLPAGDVVSRYAAALSTRRLLVFVLLAAIF
                     TLTAGQITDPDFWWHLRTGQLIFETRAIPHTDIFSFTAQGREWVTHEWLAEVLIYAIY
                     KLAGWGGLVVSFSLLMTAALWIAYRRAAELAPHPYVAGAAVLLGALATAPMWGVRPQM
                     ISFLFTSIYVAVLGDYARRGQTKGLWRLVPLMLVWANMHGGYALGLALIGLTGAGLLL
                     DEWMGERSERPLSVRLRPLLFLFAACALVVPLNPNGLRLFSYPFETLNSHAMHAYIEE
                     WFTPNFHLLISQPFALLVFATFAALALSPKKVRPGELFLLCVAAYAALRAWRNIPLFA
                     LVAVPLLAEHAWQLLESRARWRWLNEPEKREAGAKAGVQLALNVLLLICVPFGLCVAN
                     VSKVVARQGSSEAEKYPAAAVEFLRAHPESGAIYNDYGWGGYLIWKLYPQREVYIDGR
                     ADVYGDAFMEEALETMAGERNWRAPLERYGVRTVFIQPNTPLASLLRQDETWHSVYED
                     RQAVIFFKR"
     gene            complement(19707..20198)
                     /locus_tag="VGB76_21380"
     CDS             complement(19707..20198)
                     /locus_tag="VGB76_21380"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0101492.1"
                     /translation="MFLAYILCFVVAMLIHEFGHLLAARACGVGATELGLGWGRKIYG
                     LKLGGVEYKLHALPLGAYVRLDMAGLQMRPISQQVLVLLAGVIVNLCAASLATGTIFG
                     AMNYLLAATNLLPFYQQDGWKCGMVLLRELMQRRSALVEWTFTIAGGGASLALLAALV
                     LRG"
     gene            complement(20362..21927)
                     /locus_tag="VGB76_21385"
     CDS             complement(20362..21927)
                     /locus_tag="VGB76_21385"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0101493.1"
                     /translation="MSGSTLSLSSDRSENSNAPVFQLPLAAMVRALCKAATTGSDMLY
                     DVMLKLARMVDATAQTYGLATFAQKSGERPHLKWVEGLDQDEIAEAEAQVAETLAKPS
                     SVAEMKAGDHAICLVLAVATARREGVAIYGRCVRPLSERQAKELKVLCDVAQLAHAHA
                     LLREEAQPQAQLFTHPPTVATATLPGMVFVSRRMSELARAVERVKDSDSTVLVMGESG
                     TGKELVARAIHRLSRRSQAAFIPFNCTAAPADLIESLLFGHRKGAFTGAHADHDGLIR
                     AAEGGTLFLDEIGDLPLPLQPKLLRFLQEGEVHTLGERSPRRVNVRVVAATHKDLPRA
                     VAEERFREDLYYRVAALTLEVPPLRDRPEDIAALISHYLTHYARRNDRAVAGITAEAI
                     HALQNYSWPGNVRELAAEIERLVLYTDEGTSISTDLISQRISPSRSVAATTDAESGKP
                     AHLENMLDDFERRVITETLRLHDYNVARAATALGLGSRQTLYKKLKRLAINVGDFLQD
                     EQQPGLQLRAERN"
     gene            complement(22294..22782)
                     /locus_tag="VGB76_21390"
     CDS             complement(22294..22782)
                     /locus_tag="VGB76_21390"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY0101494.1"
                     /translation="MGLWDKMFGNGASESQQQPNAQQRFDTLKQKYQSVLNMADQQQI
                     QFHNLHVQDDKLFIKGTAPSDEAKGKFWDQIKLVNPNLDDITADINVDSSRAQAATAG
                     GGSQNAQSYTVKSGDTLSKISKQFYGDADEYMQIFYANRDKLKDPDKIQVGQQLNIPN
                     KS"
     gene            23068..23337
                     /locus_tag="VGB76_21395"
     CDS             23068..23337
                     /locus_tag="VGB76_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0101495.1"
                     /translation="MGSIIGAIVIGLIVGALAKAIMPGKDPGGIIVTILIGIVGSIIG
                     HYIAAAIYTSTEGPGFIMRLVFAIIGSLILLGGYRLITGRNSTRV"
     gene            23937..25343
                     /locus_tag="VGB76_21400"
     CDS             23937..25343
                     /locus_tag="VGB76_21400"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEY0101496.1"
                     /translation="MPIRFGTSGWRAIIADEFTFANVRLVTRAICSYLRTNGVEDGAS
                     LVVSYDTRFLGEKFAAQCVEEIVDNGLKAIFCDQPTPTPTISHTIRERGAAGGINFTA
                     SHNPPEYNGMKFSTADGAPALPDATRQVEGLIGSMGAASESRSRDKNTADIEVYDPRP
                     AYFADLERKLRFDVIAGAGGTYAFDPLWGTGRGYLDRALREHGLEVETIHDWRDVMFG
                     GRSPEPDDHHLDELREAMKRGGCVLGLATDGDGDRFGVLDADGTFITPNRLIPLLFDY
                     LAESRGWTGGVARSVATSHLVDRVAESRGLPVYETPVGFKFIGELINEDKIILGGEES
                     AGLSIRGHYPEKDGILACLLAAEAVAARGVSLNEQLNALYARVGRLEAGRIGVRLTPE
                     ILATLPEKLRRDPTDIGGRRVARTDRMDGVKFIFADGAWLLLRPSGTEPLVRIYAESE
                     SEKELEVLLEQGRKYLLD"
     gene            25312..25731
                     /locus_tag="VGB76_21405"
     CDS             25312..25731
                     /locus_tag="VGB76_21405"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY0101497.1"
                     /translation="MNKAANTYWTDSRLAAQRPTARPLALATPARELSRELVGTRAEV
                     RRRGGLIPSWAVFTMIMLATFALCVSVTMRTHAEMRSAEQKLSQMNTEVQAISNTNDS
                     LKQEVQRLQERKSRAIEDAARARLNMIRPNEIVVPID"
BASE COUNT         4920 a         7855 c         8057 g         5231 t
ORIGIN      
        1 tatcggggca agcgtcgaat tttgggtcga accgttgttg atgaccaaat ctctgaaacg
       61 agcacgggca cagggagcga cggggcgagt tgccgagcga gaatctacac gtacagcgag
      121 ctaaatgcaa cttgcgattg aacatttagt cgcggcagag cgctctcgcc agcaaaactc
      181 cgccgcattt caattgaacg tgtgtcctgt ggaatcaccc ttctcggatt aaagagacgg
      241 atcaacctgt tgcactaagc aacgactgtg ccaacttggc tcagacccac aagtgaagaa
      301 atataaaggc ttatctaata ggagcggttt ttcgcggaga cggcgcgacc ctcgaattcg
      361 tatcgtataa acccaccggg cacaaacgcg agcaccgcgc atcgttatga ccctttagcg
      421 gcaaaccagc ttaaaaagtt aggcaatttt gacacgctgc gcctcgcggt gtcgagaagt
      481 cgcaaaatcg ggcgcgagcg attgagtaaa attcggttct cgtccccggt tctgtgaagg
      541 cttgcggaag ttttgttgga gggtgactga gaggcgaggg ggcaacgttt gcttcggttg
      601 caaaacgcgc cggggttttt aacttcgcgg ggcgaagtag ccgaggcgtg ccgtgagccg
      661 cagggcgggg tcggaggtct tgacctgcac gcggcgaaat tttccgtcgc gcgtcttgtc
      721 gagcggcatg tagtagagcg cgtattgcga gcgcaattcc tcggccactt ccgcgtagat
      781 ttgttcgagc gcggtaaaac tgtgcggctt gaagagtcgc cctccggtgg ccgccgtcag
      841 atttgagaga ttgcgttcgc tcgcgtcgag cacgcgcagg gattcgcgca ggtcgccaat
      901 gcggagttcg ttgaagcgac gcgtggaggg gggcgtggat tggagcgtgt cgagttccgc
      961 gctcttgcgc gcacgctcga tctctgtgtt gctgatgcaa tagacgctcg ccccggcttc
     1021 gagcaacgcg ccgagcgcca tctccaaaga cgcgtggccg cgcccgctat cgatcccgtc
     1081 cgtgagcacg acgacggcat gacgacgctg cggcgcgtct ctgaatgcct cgcgcgcggc
     1141 cagatagaga gcatcgtgga aacgcgtgaa catgcccggc gtgatgcgcc ccagcgaacg
     1201 tcgcaactgc acgcggcttc gcgtccagtc ctgcaacaac tctacgcggt cgtcgaactt
     1261 caacaggctc acgcggtctt ccggggcgag ccgcgcggca aactcttccg ccgcgcgacg
     1321 aaaatcttcg aggttgctcg tcaccgaaga ggacgagtcc accatcaaca tcacgtccac
     1381 gggcacgcgc cgcacgcgca ggtcgctcaa gggctgctcg cgcccgttct cccacacctg
     1441 aaaatctttg cgctcaagcg tcgtcaccag acgccccgcc gcgtcgcgca ccgtcacggg
     1501 caggagcact tcggtcgtgc tcaccttgat cacttcgtcg tcgtcaatct cctgcgacgc
     1561 ggacggcgaa cgcttctccg tcttctgttg cccctgcgcg aacgcgacct ccgccagttg
     1621 cccccaggcc gaagccgcga caaggagcgc gagcgcacac gtagagacgc ctacgcgccg
     1681 gaggactcga aatcccgccg ggcatttaaa ccagcgcaag aaggagaaga tgaaacgcat
     1741 caatctaaac ttttacggtt tcgcacaggc taaactttta cggtttcgta caggctgaac
     1801 ttttacggtt tggcgttgaa atcattcgcg ccattcgacg ctgcgccttc ttcgttcatc
     1861 gcggcgcgca tcgcgttgat gacgggttcg ccgacgcgcc ggcggcgtag ttcagagagt
     1921 ttcgcgggcg caaaatcaaa ctcgacgggc gaactctcga tgcgccggat gatcgtgcct
     1981 tccgaaaatc ccgcttcgac aagttcgatc acggagtcgt tcgtcagcgt cggcgtgcgt
     2041 tcgagtttcg gttccgcgcc gcccgcttcc gccggtggcc gcaggatacg cacgctcgcg
     2101 ctgcccgtcg tctgtgtgtc gccttccgcc gcgccgccgc cgatgctcga acgcgagcga
     2161 ccacgcacgc cgcccgacga accgaagatg ctcgtctcgt taggatcgct ccccacgccc
     2221 gaaggcttcc ccgcatcctt cgcgcggcca gaggggcgcg tcccgtcgaa aaacggatcg
     2281 tcttcaaacg catcgcccgc cgcgtcgtcg cgcgcggcaa acgcgtcgcc ttcgctgcgc
     2341 gccagcatcg tcaggatgat ccgctccccg actccgtgct tcttcaactc gatcagacga
     2401 tccggcgaga ggtcgaagcg caccggacgc acgcgaatga tcgcgatgac ggtcttctcg
     2461 ctaaacttcg cgcgcacgag tttgacgata gagctattcg tcagaggcgt cgcctcatcc
     2521 gtctgggtcg cggacgctgc cgttgcagtc cgcgcggacg agagttgccc gcgcgtctcc
     2581 tgcgcgccgt agcccgccgc agcgacgcac aacaagagcc gcgcgccatg cctcgccaaa
     2641 cctacatgcc ttgccagcaa ccttctcata ctcctccgaa ctcgcctgtc acggctctca
     2701 attgcccgcg tctgccgtcg tcgtcctgcg ctcgcttacg gcatgcgccg cgcgcccgta
     2761 acgcccgctg atccggcgca cgtaattccg cgtctcacga tatggcggca cgcgattgcc
     2821 gtacttcgca accgcgccct cgcccgcgtt ataactcgcc agcaccaact cgacgcgccc
     2881 gttgaaccta cccagcagtt gcttcaggaa gcgcgtgccg cctgcgatgt tctgcgccgg
     2941 atcatgcacg cgccgcacgc cgaagcgcgc cgccgtcccc ggcatcaact gcatcaaccc
     3001 gcgcgccccg accgggctga cagcccccgc gttgaaatgc gattcctgct ccatcacgca
     3061 aaagatcagg tagggatcaa ctccgtaacg cgcgccgttc tgccggatca gagcatcgag
     3121 atgcggtctg cccgtcgtcc aacgacgctc gcgtttcgcg gcctgcgccg tcgtgcttgt
     3181 cgccgtcgtc tcgtctttca attcatccgt ctctctttca atacgctcaa tgcgggcgcg
     3241 ttcgagaaag atcgaaagat tgccgcgctt gtaccagacg ccttcagccg tctcgttcgc
     3301 ctcgtcaact tcaaaacgcg cgccacccac gagatagatc cagagcggac gctggacggt
     3361 ggcgacttgc gacgacttag ccgtctcatc gcctgccgcg ttcacgtctg aaagtttggc
     3421 gacctgccgc gccttcgacg cctctttctc agtccctttg cgttcgatgg tcttcacgcg
     3481 cgcacgctcg acgaaattcg tcatgccgcc gcgcctgtac cagacgccct ctgcatcgtc
     3541 ccacgcttcg tcaacttcaa tcgtcgtccc gtcaaccaga agcagacggt cggcgaaagc
     3601 aatcgcgggc agaccgagga cgaagaggaa cgcgaacgcg gcgactttca gacgcgcgac
     3661 ctgcgacccg taaaagagcg accttgcccg acggcgcgaa atttcttctt tcaacatctg
     3721 ctccccccgg atttcttaag catcaacttt caccagatcg cccacgctgc gcacttcgcc
     3781 cggcgcgccg cgacgttgcg actccgtctc ctgcgcgaag ccgtgggacg tgccgaagta
     3841 gttgcgccat gcgctgtcga tcaccttcgg cgtgagttcg atgggctggc cttcgaagag
     3901 gcagatgtcg agcacgcggt cgagcaggtc gcgcggctcg caggctttca tctggcggtg
     3961 cgagatcatg tagttgttca aaatgtagtc gaggcacacc tgctcgacgc gcatcccgcg
     4021 cgtgtgcgcc gcgcgcttga agatttcgat gtaggcggcg ggcgttggcg gttcgacccg
     4081 cgcgcgatag cccatgcggc gcaggaaagc ctcgtcaacc aggtctttgt cttcgaggtt
     4141 cgtcgcaaag acgacgagtt gctcaaacgg cacttcgatc ttcttgcccg tgtgcagcgt
     4201 gagataatcc acgcgacgtt cgagcggcac gatccagcga ttcaggaggt ctgcgggcgc
     4261 gacgcgctgc cgcccgaagt cgtctatgac gagcgtgccg ccgttcgact tcatctgaaa
     4321 cggcgcttcg taaaaacgcg ccgcctcgct ccacgtcaag tccgcgttct ccagcgtcaa
     4381 ctcgccgccg acgatcacga gcggacgatc aatgcagacc caacggcggt cgtattcggc
     4441 gggcgtcgca tcgtttgcgg cgggacggtg attgtgcgcg tcgaagacgc gtatgacctg
     4501 accgtcaatc tcgatggcgt agggaatcca gatcgcgccg gggagactcg cgttgatgcg
     4561 ctcggcgacg gcggttttgc ccgtgcccgg aggaccactg aggaagagcg accggcgcga
     4621 gttgacgacg cagcccaccg tctgcatgag cgaatccggc aggatgagat cgcggaaggc
     4681 cgcgcgcacc gagtccatcg tcgcgtggcg cgcggggacg gcttgcaggc gcatcatgtg
     4741 cgcgtagtcg cgcagggaga cgggcgcggg accatcgtag ccgcagaagg cgcgtgcgcg
     4801 tgcgaggcgt tcccagccct tatcgagcat gccgtagcgc gttgcgccca tcgcggattg
     4861 cagtttcacc tcgatcaatt tctcgcggta gagctgctgc aaaatctctt ccgtcagagc
     4921 gcgcggcagg cgcagacgct ctgtgacgtt ggcggtcgaa ggctccggca gcgcggccac
     4981 gatcttgagt gcgaggtcgc acaggaaact ctccggcacg tccgtctcgg cgagttcttc
     5041 cggcacgggc ggtcccatgc tctccacttc catgcgcgtc agtatgtttg tttgcgtcat
     5101 gtctcgattc cccgttcgat tcgctgtcgg cgaagttgat tattccttac cggcgacctg
     5161 ttcattggcg cgcccgtcga gcgcacggcg cgtctgttcg gcgcgctcgc cctgcccgtt
     5221 gcgcgcgtag agttcgacga ggcgacgcag gacgaactcc gattgaggct gcacgcgacg
     5281 cgcggcctcg tagtgctcga tggcctgatc ttcgcgtccc gcgcgttcga gcatggcggc
     5341 gacgttgagc cgaccctgat actcgccgcc ggaacgcgcg aagctcttgt aagcatcgtc
     5401 gtatttgccg aggcgacatt gcgcgagcgc gagattgttc cagatgcgtt cgtctgcggg
     5461 cgcgagcttc gcggcgcgct tcaagcgcga gatggctgcg cgatagttgc cgctcaggta
     5521 gagcgagtag ccgaggttgt tcaaggtctg cgcgtccggt tcgtggtcga gggcgcgtgc
     5581 gaaagattcg cgtgcgcgtt cgggcagccc tttgcggtcg taggcgacgg ccagcaggtt
     5641 gtgcgcctcg gacaggcgcg catcgagcga gacggcgcgc gacagttccc tgatcgcttc
     5701 gttcaggcgg ccttcatcca gacgcgtgcg accgcgtgcg agataatctc tggccgtgcc
     5761 gccttcgtcc gtgtggctct ccgcgctcgc cgtgcggctg tcttccacgc gcatcaaggg
     5821 cgcgctctca aacttcgcca cgtctttctc cgacagccgg cgcaccttgt cgtcgtcgcc
     5881 gccgaagagt ttcgccgccg ctttgaaagg ggctttcagg aaacgcgcga aacgattgcc
     5941 cttcttcttc tgcttctctt cgctgcccgc cgccgcgtct tccgtcgcac ccgtgggcga
     6001 gccttcggag gcgacgacgt tggccgcgtc gtctccgtcg cgctcggtcg ttccggcagt
     6061 ccagaaagtc gtgacgctga tggttaagac aagggcgatg atgaaagcgt tgatggtgcg
     6121 atggcgcatg attgaagact cctttacatc ttggcgaacg aatcaatgag attgagcgcg
     6181 gcggggccga gaatggcgat gaagagcgtc gggaacagga agcacgcgag cgggaagagc
     6241 aacttgacgg cggccttctg cgcggcctgc tccgcgcgct gcctgcgctt cgtgcgcaag
     6301 ctgtccgcat agacgcgcac cgcacgcgag attgatgtgc cgaagcgatc cgcctgaatg
     6361 agcatcgcca cgaggctgcg caggtcgtct acgccggtgc gttccgccag attgcgcagg
     6421 gcttcgtcgc ggtcgcgccc gacgcggatt tccagattgg tcaactcgaa ttcttcgctg
     6481 atctccgggt ggacttcgcg caactcttcg cccaccttca caatcgcggc gttcaaaccg
     6541 agtcccgcct cgatggagac gaccatcagg tcgagcgcat ccgccagccc ccagcgcacg
     6601 cgctgctggc gactcttgac catcttgcgc aacgcgtagc gcggcagaaa gaagcccttg
     6661 atgaacgccc cgagaatcca catcatcgcg ctgctgagcg gtcgcgccgt gagcgcgcaa
     6721 gcgagcgcga cgatggcggg ataaccgatg agcgtggaga tttgaatggc gcgatagacg
     6781 attggcgcgt tcggcgaacg atacccggcc tgcatcaact gcttttgcag tttcttcgct
     6841 tcggcagccg aagggggcgc gagccggtgc agaggtttcg ccatgcgttc ggccagcgcg
     6901 gtcatgccgt cgtccgacgc ggcgttcgcg ctcaggtcta cgccgcccgc gcggagaacc
     6961 gcgccgccgc cgcctgtctc gcccatgcgc cgcaggcgtt cggtcgccgc gctcgccggg
     7021 cgaaacatca accagtagat ccccagcacg ccgagcgtga tgaacgcgaa tgtcgagagg
     7081 gtgataatga gaatcatatg tttaagtgac gagtgacgag tgacgagcaa gaaccagtta
     7141 aaactgttgc cgtcgtttcc gactttcgtg cgactggcgg gtgatgagtt caagggcaag
     7201 ttttaagaga aggcttctgc tttaactcgt ccctcgtcac tgcctttcaa attttgatgt
     7261 tcaaaatttt cctgatcacg agcatgcccg tgatctgcaa gccgatggcg acggccatca
     7321 gtttgtggcc gcgcgggtcg ttccacatga cgctcatgta ttcgggattc atcaccgtga
     7381 cggcgagcgc gacgaagatc ggcaggccgc agagaatcca cgcggacatg cgcgaactcg
     7441 tcgtgagcgt cttcaaatct tccatgatgc ggaagcgttc gcggatcgtg tgcgccactt
     7501 tttccagcac ttcggcgagg ttgccgcccg tctcgcgctg gatgagaatc gccgtgatgc
     7561 acaatttcag atcgagcaag ggcacgcgcg cagccagatt gtcgagcgcg agtttgagcg
     7621 agaggccgag gttctgttct tcgtaagtct tgcgaaactc ggtggcgatg gggtcgggca
     7681 tctcggtcgc caccatgtgc agggcttcgg cgaagccgtg accggcggag aggccgcgac
     7741 tcatcagttc gagcgtgtcg ggcaggtctt cgagaaactt gttgagccgc ttgcgccgcg
     7801 cccagacgac gtggacaaac ggcaccacgg cggcagccgc accggccatc acgatcagga
     7861 tggtcgagat cgtgagcatc gaggcggcca gcccggcgag catcgcggcc atgagcgaga
     7921 acatgaagag ccgcgtcacg gtgatgtgca ggtcggcctg atcgagcatg cgcttgagct
     7981 tcgtcgccac ctgcacgcgc acgagcatgc ggtccagcag cggtatctcg ctcatcaatt
     8041 cctgacgcgc gagctgcacc tctacatccg cgtcgtgcgc cgaatggagc agagcttccg
     8101 acaggcgttg ctgcaaacgc acacgcttct tctccgagcc gcgcgtcgca agcaggtagc
     8161 ccgcgaaggt caggaacaag cagaaggcaa agacgaaaaa tggaatcata agagcaccgg
     8221 aattcaggag tcggaatgca gaaaacagaa tgtagaaatc aggattgttg tcggcaaaag
     8281 ttgtcgccga taactgctgc ggctttcctg ctgactcctg aattctgtgt tctgcattcc
     8341 ccgtttaatt ctcttcaaaa aagactcgcg gcaactgcat gccgtaaact ttgaggcgtt
     8401 cggcgaagcg cgggcgcacg cccgtcgggc ggaagcgacc gacgacgtgg ccgtctttgt
     8461 cgatgccctt gcgctcgaac tggaagatct cctgcatcgt gatcgtgtcg ccttccatcc
     8521 ctgtgatctc ggagatggag acgacgcggc gcgtgccgtc agagaggcgc gcggcttgaa
     8581 tgacgagatt caaggcggaa gcgatctgct ggcgcatggc gcgctcggag agtttcatgc
     8641 ccgtcatttg aatcatcgtt tcgagtcgcg ccagcgcatc gcgcggggtg ttagcgtgca
     8701 cggtcgtcaa actgccgtcg tggcctgtgt tcatcgcttg cagcatgtcg atggcttcgc
     8761 cgccgcgcac ctcgccgatg acgatgcggt cggggcgcat gcgcagcgcg ttcttgacga
     8821 gatcgcgctg cgtgacttcg ccgcgcccct cgatgttggg cgggcgcgtc tccagacgga
     8881 cgacgtgcgg ctgctgcaac tgaagttccg ccgagtcttc aatcgtaacg atgcgctgct
     8941 cttcggggat gtaggcggag agcgcgttga ggagcgtcgt cttgcctgcg cccgtgccgc
     9001 cggagatcag tatgttcagg cgcgcgttga cgcacatctc gaacatgtcc gcgatgtctt
     9061 tcgtgagcgc gccgatctcg atgagcttcg ccatgcgcag cggctccgcg ccgaaacggc
     9121 ggatggagag cacgggacca tcgagcgaga gcggcgggat gatggcgttg acgcgcgagc
     9181 cgtcagagag gcgcgcgtca accatcggtg aggattcgtc gatgcgacga ccgacggacg
     9241 agacgatgcg ctcgatgacg cgcatcaaat gttcgtcgtc tttgaaggcg acttcggtgc
     9301 gttcgagttt gccgcgccgc tcgatgtaga tgttgtgcgg cgagttgacg agaatgtcgg
     9361 agatggtcgg gtcggccagc agtggttcga ggggtccgag gccgaacaac tcgtggcgca
     9421 cttcttcgac gaggcgttcg cggtcgctgg tcgagagcgg catgttctcg atggcgagca
     9481 agccttcggc gagacgcgac acttcggcgc gaagctgatc ggcgtcgagc tggctgagct
     9541 tcgtcaagtc catccggttg atgatcgcgc ggtgcaggcg cgacttcatc tcctggaaag
     9601 tgtgctgccc gcccgtcgcc gcttcgtcgc ccgtgcgcgc caccggcatc aacgccgggt
     9661 tggtttctct aactaatctt tcgcgtagtg acatgatgat tttgcccgcc ggatgttcgt
     9721 tcaagtttga cgattcagga tttgaaaact ggtactgcgc gcgcccttag atcgcggcgg
     9781 tcgctttgtc tttgttcagg cgtgaacgca agcccacgtt tgagatcgcg ccgaccggac
     9841 gcccgaagat cgcgccccac acgcctttgc gcggctcctt gatctcggat tcgacgctcg
     9901 cgcccgtgat gctcgcagca atgtggcgta tctcctgcgc gatgcgcgaa gtggggttgg
     9961 atttaacgag cggctcgccg agattgatcg agttgacggc agtgttgtag tcgctctgca
    10021 cgtagcccgt cacgcgttcg cctaagaatc tttcgacctg acgcagatcg agatcgatct
    10081 gtttgctcca gcggttgacg acgatgcgga ctttgtggcg cgggtagccg agccggtcga
    10141 agagcgcgag cgcgcgttgc gtgctgcgaa tcgcggggat gtcgagcgtc aggacgagca
    10201 ctacctcgtc tgcctgatcg agcgcggaga gcgtgatcgc gtcgaaggtg tgctgcgggt
    10261 cgatgacaac gtagtcgaag cgttcgcgca ggagttcgag cacttcgaag atgtgttcgg
    10321 gttcgatgtc gtccgcgagg tcggcttcgc ggggcgcggc cagcagggag aggttcgagg
    10381 tgtggggcat gacgtagctt ttcaggagcg agtcgtcaat gcgcgcgcgg ttttcgacga
    10441 gatcggcgat ggagaatttc ggttcgaggc cgaggaagag cgagaggtcg cccgcctgca
    10501 agttgagatc gacgaggacg gtcggcgcat cgagcgcggc ggcgaggttc gtagcgataa
    10561 aggaggtgcc gcagccgccc ttgctggaaa agaccgcgac ggcgcgaccg cgtctcttcg
    10621 gcgcggccac ctgcgtcgag cagaattcgg cggtgcgctc aatcactgtc tgaaattctt
    10681 cggcgatgac gggcaggcgc agaaagtcgc gtgctccggc gcggtaactt cgcaggatca
    10741 agtcgggcga ggcgtcgcgc gaggcgcaga tgatcgcggt ctgcgggcac tctgcggcga
    10801 gtcgctcgac gaatttcaag gccgtttccg gctgcgcgcc gaacgtgatg atggcggcgt
    10861 tcgggcgcag gcgcacaacc tccgggaaga gttgttcggc atcgtcgccg cccgccagca
    10921 ggcgcgtctg ttcgttggcg gcaagcgcgc cgcgcaactc tttcagggat tcgagtccgg
    10981 tgctgaggat gacgaatgac agttgattca tgtttggcgt tctctcgttg aataagatga
    11041 atcggagatc ggaagctgcc tgcgcggcga taacgcgttc agggaatgac tgtgggcacg
    11101 tagcccgcgt tttcgccggt cagcgaggtc ttgtaagcgg gcaggcgcat cgtcttgttg
    11161 acgagcggca acacgaattt gaaatcgtag ttttcgatct cggctgtgac ggtgccgccg
    11221 ccgagttcga aattttgata agtgagcttc acgtgggtcg gctcaaggtc ttggacgagc
    11281 ggttgacccg tgccgcccgt gttgccgtag acggccatgt tcttcgcctg cgtctgcgcg
    11341 ccggtgctca tgccctgatt gacggcgtag cgcgcggcgc ggcgcgtggc atccaccagc
    11401 gagttgtgta cccagagcag acgcccgact tcgatgacgg cgaaggaggc ggagagaaag
    11461 agcgtcgcgc cgatggcaaa ctcaaggagt gccgccccgc gttcgtcgct tcgtcggaaa
    11521 atttttgcgc gcatcgcttt catcatctct tcaaggctcg cttcaattga cgagttgccg
    11581 cattgtgctc ttcgcgccga cgttgacgct cagagagagc gtcgaactcc gtgtgagttt
    11641 gccgaggtcg aagatgggga cgtaggtgta gttctgaatc tcgaccgtct gtgttttcgc
    11701 gcccgccttg ttcggcgtga tgacgacgtt ggcggtcgtc aaaccgctcg ccacgggcgt
    11761 tcccgtgccc gccgtgttgc cgtagaccaa caggttacgc gccgccgagt cggccttgcc
    11821 caccggttgg ctgacgaggt aacgcgaggc cgcgcgcgtt cctttcaaca gcgtcgtgta
    11881 ggtgtagaag aagcgaccga actccgccgt cgcgcccagc aagaccagca ggagcggcat
    11941 gatgatggcg agttcgacga gttgcgaacc gcgctcgcag tcgcgcaatg cgcggaggcg
    12001 tcgcccgcag gcgcgtgtga atctttgtgt gagttcagct tgactgcgca tcgcaaagca
    12061 ccctctagcg ataaagaacg gggatggtga gttcggggct gaccgcgccg ccgttcgggt
    12121 cgtaaccgcc gcgaccgagc acggtgcgga tgccgatgta ctcggccttg atgtcgccgc
    12181 cgttgccgcc gtcaactttg gtttgcagga agaaggccgc gaagcgatca atcttcacgc
    12241 tatcgcgccc gccctcgaac tcggctatgc ggatgatcgg gatgagcacg acgcgacgac
    12301 cgcgctccgg cactccgcgc gtcggcgctt caaaggtgtt tgcggcgggc gtcttgtcgg
    12361 catcgatggc ctgctgcgcg tcgagatact gttgatagtt gatgtgctcc tgcacgttct
    12421 cgtcgggggg atactcggag gggtcgagtc cgccgccata ctcgccgaag cgcgtgttga
    12481 tgccctgcct gaccgcgccc gccgaaacgc ccggcttcgt cttcacgtac tcgcccgcgc
    12541 ccacgcaatt cctcacgccc ttgcccagcc cgatgcggtc gtcgctcgcg cccggcccgt
    12601 cgatggcgag aatctggtag ttgcccggag agactgcgcc gcccggcggg cgacggatgg
    12661 tgtaggtctg ccccttcacg aaatagacat catcggcatc aatggccgtg accggaatgt
    12721 aatcgcagaa cctgttgagc gagacgctca tgccgcccgt agcgtctgcc gccacgttgc
    12781 gcgacgcgcc gagtacgggc gcggcgaaac tcgtggcgac ggcggcgggc ggaatcatga
    12841 ctttcacgaa gcgtatgtcc ttcgcctgtc ccttcgccgt cgcctcgctc acgtaggggc
    12901 cgttcagatt tttcgcaaac agcacgttct cgcgcgggat cgtgacgccc ttcttgttga
    12961 actcgtagct gttcagagat tggacggcgt agttggcggc ggtcgtgatg ccgccatcgt
    13021 ccgagttgag cgcggacgcg cccgcaagcg cggcggcgtc ggcggcgttc tgcaactcgc
    13081 cgcgcacgag atagaagtgg ctgatgtcta tgcacaggcc ggtggcgagg aggaaggcga
    13141 gcatgccgaa ggtgctgatc gccagtactg aaccgcgctc gccccgacgc tctctcaaac
    13201 tctctttctg actcgttcgt ttcatcgctg ctctctcctc tccccggaca tccgccagcc
    13261 tctaactctc acgcgactct tacccgcaga ctgcctactg cttcgcgctg accttcgccg
    13321 tagacgacag cagaggcgcg ttctgcgtcg cgccgaaata actcgacagg acggcgtgcg
    13381 ccgcgttgcc ggcaggcggc aacacagctt cctgcgccgt gttcgcggcc tgttctttca
    13441 atgccgcctt cgccgtctcc ttcgacgcgt ccttctcttc cttgacggca tccttcgcag
    13501 gcgtcgtcgt cgccggactc gccaacggac tcacggcggg cgcgactgtc tgcgtcgcgg
    13561 tgttggcatc ggtcgcgctc gtcgctttcg ctccgtccgt gcccgtcgaa taaccgctcg
    13621 cgcccttgat tccttcgccc tgcggttcga cgccgagcgg cgagcctttc ttcaaaccgt
    13681 cgaggccgcg cgtggcgggc aggtcgtcgc ggttcacggg cttgacgagg gtggccgtgc
    13741 agaagaagac gagttccgtc tcacgcttct cgaacgattt cgatttgaag agcgcgccga
    13801 ggatggggat gttgccaagg ccgggcactt tcgagagcgt gctcgattcg ctgttgtcga
    13861 gcagacccgc gagcgcgaag ctctggccgt cgcgcagttc gatgccggtc ttggcgcggc
    13921 gcgtcttcag ggcggggatc aaatagcctt gaaacttgac gccgttctgg aagtcaatcg
    13981 tcgagacttc gggttcgagt tctaactgga tgtggtcttc gtcgatgatg gtcggtttga
    14041 agttcaggcg cacgccgtac tctttaaaga ccaccgtgac gttcacgcgc tggccgtccg
    14101 attgaatgac ggggatcgga aactcgccgc ccgccagaaa gctcgcctgc tggccgctca
    14161 tcgcgatcac gttcggttct gcgagcgagc gaatcgcgcc ggaggtttgc agcgcgcgaa
    14221 tggccgccgc cgagccgacg cccgtgttgg agatgaagag attcgccgtc tcgaacaggg
    14281 aggcgaggct gttggtcgcc gagacgctcg tgccgttgcc cgacgaagcc catgacgaac
    14341 ccaactcgcg cagcttcgtg cggctcactt ccgccacacg caccgacaac tgcacctgca
    14401 tcgcgttctt cgcgggcgaa gtcagcatgt tcaccgtctg gaaccccgcg gccttcacca
    14461 cctgctcaac ctgcgtcgag gtctgcgcct gcgcgacgct gccggagatc acgaccgacc
    14521 cgttcgcctg gctcaggcgg atgtcgtcgt tcgggaagag ggcgcgaatc tgcgaatcta
    14581 tgagagagag gttggcgcgc acgaacacgt cgaagacgac gaacttgccc gactctttct
    14641 cccacgcgat gaagttgacc tgaccgaaag ccttgccgtt gacgagcacc tggtccggcg
    14701 cgacgagcac ggcctcggcc acttccgggt tcgacacgga gaagcgtccg atcttgcggt
    14761 cgaagttgat cacgcgcgac tggccgacca gcacgttgat cgccacagac tctttctgcg
    14821 ggttggcgaa cgacgcgctc acgctcgttt cctgcgcgtg cgccgcgccg gtcacactga
    14881 aaatgaacga cgcgcacagc atcgcgatga taaaggggat aagacgacgg gtctgcatga
    14941 ttggactcct ttgaaaaagt aagcagtggg ctgtaagcag tgagcggtct gtcgcattgc
    15001 agcgcgcgat gagcagcagg ctttgaactg ccggctgctc atcgccgtct gcttactgtg
    15061 ggaaatctac gtttcgtttc ttcgcgcctt cgatcacttc gatggacgag cgcgggggag
    15121 caggagcagg ggcagccggg ggagtggccg cctgcggcgc gggtgcgctg ttgctgctcc
    15181 tgctgttgtc ggcgcgggga tacgagcggc gcatgacggg cgcgggctgt tgcgtctgct
    15241 gcggttgctc actcttgagc gaaccggcct cgggcacggg cttggcgcgt tcgccgccga
    15301 ggagcacgcg cttgtcaacg ccgagcgtct gctcgtcgcc ttggtccacg gagttgcgca
    15361 tcacgagttg gagtttgcct tcggtcgagg cgagcgcgag tttctctgcc tgttcgggca
    15421 ggacttgcag cgtcacggcc ttaacgctct ccggctcgcg gtcgttcttc gggcggtcta
    15481 tgttctgccc gttggcgagc accttgatgt tctgcaagac gatcttcgag atcgggttct
    15541 gctggccgtt ctgctcgacc ggattgatca ccaccaccac atccacgagc gcgcccggca
    15601 tgacgaagcc ggagatgccg accacgtcgt caaccttcac ggtcatcgcg cgatagcctt
    15661 cggggataac ggcggacagg ccgcccgcgg agccttcggg cgcgagtttg aaatcggtga
    15721 cgggttcgcg cgccgcgatc tgcacgaccg ccacgcgacc gacgagtttc tccatcgact
    15781 ggaatgtccc ttcgggcatc gccgtgctcg gaaactgcac gctctggagt tgctccgcga
    15841 tgatcttttc gcccggctgg atatcgacct tggcgaccac gaccgtgttg agattcttcg
    15901 cgtaagcctg cgtgcccgac aggtaacggc tcaccgaaaa ggccgcgatc aggccgaaca
    15961 ccaccgcgcc gcacatcaca aagacaagac gtttatttcg catggctatc attctccgtt
    16021 cgagttcaaa ttcccctgcg cgctccccgg tggcttccgc agccccggcg cgcaatattt
    16081 ttcgttaacg tttaagcgcg aaagagtgcg agcgagatca ggctgccgag cgtgatcgcc
    16141 acgccgtaag gaatggtgta acgacgttct gccggaaccg agaagcgcgg catctcccaa
    16201 ccgggcagga agcccacgaa gatttgcacc acgcgccaca gggtttcgcg caccgtcccg
    16261 gcgcgaaaca tcgtcacgac cgcgagcgcg ccgcccgtca gcacgacgac gagaaaggtc
    16321 ggcacgacga gcgacgcgcc gagcagcgag ccgatagccg cgaacaattt cacgtcgccc
    16381 gcgcccatcg cgccgaagat gtgcagcatg aacatcaggg cgaaggcgag cgcgcaaccg
    16441 ccgagactcg ccagcaggcc gtgcgtccca ttgatgatcg tgttgacgga gagccctgcg
    16501 acgagcgtgg cgaggacgaa agcgttgggg atgcggcgat agcgcgcgtc gtaataagtg
    16561 atgacgacgg cgagcgggac gagtaatgcg ctgctgacga tggttaaagt gcctgacatg
    16621 tcgtgtatga aaattggttg agttgaatta aatgcgcgac tgagttttta agtgctggta
    16681 aaacttttac tgctggtaaa aaatggttgg cgcgctccgg ctgtttctgt gagaagcggc
    16741 gagagcgcgc cagccctgtt ggagtcccgt gtcagggggt ggaggcgatg ctggttgcaa
    16801 gattcgtgat cttggttccg atgttcgtgc cgagcgtcgt gaaggccgtg atgacggcga
    16861 gcgcgacgag tgcggcggcg accgcgtatt cggaaagttc cagcccggtt tcgtcttgaa
    16921 agaatttctt gaccatttca cttgttcctc actgcaaaga ggttttgacg ggaaggctta
    16981 cggggtgccg atcttcgtcg ccagattgtt gatcttggtg ccgatgttgc caccgagcgt
    17041 ggtgaaagcc gtgatgacgg ccaaggcgac gagggcggcg gcaacggcat actcggagag
    17101 ttccaatccg gtttcgtcgc gcatgaattt cttgatcatt gtcttttgct cctgtgtctg
    17161 gaagtggaat ttgaagttgg attattacgg ggcgatcttg gtggcgagat tgttgatctt
    17221 cgcgccgatg ttcgtgccga gggtggtgaa cgcggtgatg acggcgagcg cgacgagcgc
    17281 ggcggccacg gcgtattcgg acagttccag accagtttcg tcgcggagga atttcttaat
    17341 catttggttt gctccttcaa tgcgggaatg tttcgtgttt gttcttgcag acgctcgcgc
    17401 cgagtgggcg ggcggccaac tacatcatca aagaaccgga tgcttcatca aaccgtgcca
    17461 cgaactgaca agcacgccgc caatcgccag ggcgacgagc agccagttgc cgttcgctcc
    17521 cccggccacg accgccacgg cgcgctcacc cgccagcagc gcaacggcta cgaacggcag
    17581 gacgagcaac agggcggcga cggctaagaa tgttttgttg agcagggttt tcatcatcga
    17641 gttcctttcg cgcccggcgc aacgcttcga gttgccacac ctttcaacgt tgcttccgga
    17701 cgacagaagc tatttcaagg cgcgtgccac acgtcggaaa ctcgaagaac acaggcaaac
    17761 gcggctttta cgcatctcgc gccgcgacgc catgtgaacg cagggcgaca acaaagcgcg
    17821 tcgccgacgc acgactgcgc cccccgcttt cgagtaaaac tgagcagtga gaagcactta
    17881 acgccttgtc gccgccggtt gacaaagttg cgcgcgagtt tcaaccggtg gcgacgagcg
    17941 ttgacgagaa agattgagag aactgatgcg agcggcgtgc gcgtgcgagc cgcgctaaaa
    18001 atttaaggga cgcagcacag gccgcgtccc tctcaagtcg agcttattaa cttgcttgtc
    18061 gtccgtctct aacgcttgaa gaagatgacg gcctgacggt cttcgtagac gctgtgccac
    18121 gtctcatcct gccgcagcag gctcgcaagc ggcgtgttcg gttggatgaa gacggttctc
    18181 acgccgtaac gctccagcgg cgcgcgccag ttgcgctcac ccgccatcgt ctccagagcc
    18241 tcttccatga aggcgtcgcc gtacacgtcc gcgcgcccgt caatatagac ctcgcgctgc
    18301 gggtaaagtt tccagatcag gtaaccgccc cagccgtaat cgttatagat cgcgcctgac
    18361 tccgggtgcg cgcgcaggaa ttcgacggcg gctgccgggt acttctctgc ctcgctactc
    18421 ccctgtcgag cgacgacctt cgacacgttc gccacgcaca agccgaacgg cacgcagatc
    18481 aacaacaaga cgttgagcgc caactgaacc ccggccttcg ccccggcctc gcgcttctcc
    18541 ggctcattca accaacgcca acgcgcccgg ctctccagca gttgccacgc atgctccgcg
    18601 agcaacggca cggcgaccag cgcgaagaga ggaatgttgc gccacgcgcg caaggccgcg
    18661 taagccgcga cgcagagcag gaacaactcg ccgggtcgca ccttcttcgg cgagagcgca
    18721 agcgcggcga acgtagcgaa gaccaagagg gcgaacggct ggctgatgag caggtgaaag
    18781 ttcggcgtga accattcctc tatgtacgcg tgcatcgcgt gcgagttgag cgtctcgaac
    18841 gggtaggaga acagcctcag gccgttcgga ttcaacggca cgacgagcgc gcacgccgcg
    18901 aacagaaaca acagagggcg caggcgaacc gacagaggcc gctcgctccg ctctcccatc
    18961 cactcgtcga gcaacagacc tgcgcccgtc aagccgatca aagccaagcc caacgcgtag
    19021 ccgccgtgca tgttcgccca gacgagcatc agcggcacga gccgccacaa acctttggtc
    19081 tgcccccgcc gcgcataatc gccgagcacg gccacgtaga tgctcgtgaa gagaaacgag
    19141 atcatctgcg ggcgcacgcc ccacatcggc gcggtcgcca gtgcgccgag gagcaccgcc
    19201 gcgcccgcca catacgggtg cggcgcgagt tcagccgcgc gcctgtaagc gatccacagc
    19261 gcggcggtca tcaggagcga gaaggacacc accagcccgc cccatcccgc cagtttgtag
    19321 atcgcataga tcaaaacctc ggcgagccat tcgtgtgtca cccactctct gccctgagcc
    19381 gtgaaagaga agatgtccgt gtgcgggatc gcgcgcgtct caaagatgag ttggccggtg
    19441 cgcaggtgcc accagaaatc gggatcggtg atctgccccg ccgtcagtgt gaagatggct
    19501 gcgagcagga cgaagaccag caaccgccgc gtcgaaagag ccgccgcata tctgctcacc
    19561 acgtcgcccg ccggaagaac gaccggccac gcggcatcgc ccgctttttc aaactccctc
    19621 atcgccgcgt cctcattcgt caaacgcatc ttccaacgtc aacttcatca gctacacact
    19681 cgccgacaac ccgcgccggt tgttcatcaa ccgcgcaaca caagcgcggc cagcaacgcg
    19741 aggctcgcgc cgccgcccgc gatggtgaac gtccactcga caagcgcgct gcgtcgctgc
    19801 atcaactcgc gcagcaacac catgccgcac ttccaaccgt cctgttgata aaacggcagc
    19861 aagttcgtgg ccgcgagcag gtagttcatc gcgccgaaga tcgttcccgt ggcgagcgag
    19921 gccgcgcaca ggttcacgat cacgcccgcc agcaacacca gcacctgctg gctgatcggc
    19981 ctcatctgca agcccgccat gtcgagccgg acatacgccc ccagaggtaa cgcgtgcagc
    20041 ttgtattcaa ctccccccaa cttcaagcca tagattttcc gcccccatcc gagtcccagc
    20101 tccgtcgctc cgacgccgca cgcgcgcgcc gccagcagat gcccgaactc atgtatcagc
    20161 atcgcgacga cgaagcagag gatgtaggcg aggaacataa attttgtcag ttgtccgtgg
    20221 ttaactggga gaggtcaatt gtcagaggtc aatttgtcgg ttgttctttg ttcgctgccc
    20281 gacgcttatc gtcatccccg gagagtaacc tgcccgttga caaaacaacg ggcggctgac
    20341 cacggacaac tggcggctct cttagtttcg ttcggcgcgg agttgcaggc cgggttgttg
    20401 ttcgtcttgc aggaagtcgc cgacgttgat ggcgaggcgt ttcagcttct tgtagagcgt
    20461 ctggcgtgag ccgaggccga gggcggtggc ggcgcgtgcg acgttgtagt cgtgcaggcg
    20521 tagcgtttcg gtgatgacgc ggcgttcaaa gtcgtcgagc atgttttcca gatgcgcggg
    20581 cttgccgctc tcggcgtcgg ttgttgccgc aacggagcgc gacgggctga tgcgctggct
    20641 gatgagatcg gtgctgatgg aagtcccctc gtcggtgtag agcacgagcc gttcgatctc
    20701 tgcggcgagt tcgcgcacgt tgcccggcca cgaataattt tgcagcgcat ggatcgcttc
    20761 ggcggtgatc cctgcgacgg cgcggtcgtt gcggcgcgcg tagtgcgtca ggtagtgaga
    20821 gatgagcgcg gcaatgtctt cgggtcggtc gcgcaggggc ggcacttcga gcgtcagagc
    20881 ggcgacgcgg taatacaggt cttcgcgaaa acgctcttcg gcgacggcgc gcggcaagtc
    20941 cttgtgtgtg gcggcgacga cgcgcacgtt cacgcgtcgc ggcgagcgtt caccgagcgt
    21001 gtgcacttcg ccctcctgta agaagcgcaa aagtttcggc tgcaagggga gcgggaggtc
    21061 gccgatctcg tcgagaaaca gcgtgccgcc ttcagccgcg cggatgagtc cgtcgtgatc
    21121 tgcgtgtgcg ccggtgaacg cccctttgcg atggccgaag agcaggctct cgatcaaatc
    21181 tgcgggcgcc gccgtgcagt tgaacggaat gaacgccgcc tgcgagcgac ggctcaagcg
    21241 atgaatcgca cgcgcgacga gttcctttcc cgtgcccgat tcgcccatca cgagcaccgt
    21301 cgaatccgaa tctttaacgc gctcgacagc ccgcgcgagt tccgacatgc ggcgactgac
    21361 gaagaccatc cccggcaaag tggccgtcgc aaccgttggc gggtgcgtga acaattgcgc
    21421 ctgcggctgc gcttcttcgc ggagcagcgc gtgcgcgtgc gcgagttgcg ccacatcgca
    21481 gaggactttt agttccttcg cctgccgttc ggagagtggc cgcacgcagc gcccgtaaat
    21541 agcgactcct tcgcggcgcg cagtggcaac ggccaagacg agacagatcg cgtggtcgcc
    21601 cgctttcatc tccgcgacgg acgaaggctt cgcgagcgtc tccgcaactt gagcctcggc
    21661 ctccgcgatc tcgtcctgat caagtccctc gacccatttg agatgcggtc gctcgccgct
    21721 cttttgcgcg aaggtggcta agccgtaagt ctgcgccgtc gcgtcaacca tccgcgcgag
    21781 cttcaacatg acatcgtaga gcatgtctga gccggtcgtc gcggctttgc acagtgcgcg
    21841 caccatcgcg gcgagcggca gttgaaagac aggagcgttg ctgttttccg aacgatcaga
    21901 actcaaggaa agcgttgaac cggacatgaa attatgaatc ccctatgctg aaaaacgaca
    21961 gacggcagct tcgagcaggt gcgacgccaa ctcgcgcgag tgaagtttgt ttggagtcag
    22021 ggcaagtctt tatgacgaga aattcgggga gcggctcgtc ctgccgaaga gcacgtgagg
    22081 cggcttcgac tcaaactaaa attagtctag tcgcacggcg cggcgggtgt caataaaatt
    22141 aagaaacgcg cttgtcgctg aaagcgaagg caatcgaaat ataacaatca ggcgaaaaca
    22201 ggacttgctt ttaacgcgaa agagcgtcgc ccctctacaa acatagcggg cgacgctctt
    22261 tctggtttta ctcagacagt ggaaaactca aacttaggac ttgttgggaa tattcaactg
    22321 ctggccgacc tgaatcttgt caggatcctt cagtttatcg cggttcgcgt agaagatttg
    22381 catgtactcg tcggcgtcgc cgtagaattg tttgctgatc ttcgagagcg tatcaccgga
    22441 tttgaccgtg tagctctgcg cgttctggct gccgccgccc gccgtcgccg cctgcgcgcg
    22501 gctcgaatcc acgttgatgt cggcggtgat gtcgtccaga ttcgggttga cgagtttgat
    22561 ctgatcccag aatttgcctt tagcctcatc cgagggcgca gtgcccttga tgaacagttt
    22621 gtcgtcctgc acgtgcagat tgtgaaattg aatctgctgc tggtcagcca tattcaaaac
    22681 cgattgatat ttttgtttga gcgtatcaaa gcgttgttgc gcgtttggct gctgctgcga
    22741 ctcgctggct ccattgccga acattttatc ccaaagaccc ataaagattt cctcctccaa
    22801 attaaagaaa cgattttttt ccaaacaccg aaaggcgttg ccgggttaca ggtgcaacgc
    22861 acgtaccata aaacggaatc cgtccggcta actttcaatg actcgactat tagcgtttga
    22921 atcgacacaa aacgctctgg cgtgcgcctt gctaactggt caaacgcaac tgattttcta
    22981 gcttgaaatc gtacagttgc cgatttggtg gctgctttcg agcagttttg agtattctaa
    23041 aaacgatttg aacaggaggg atgcgaaatg ggttctatta ttggagcaat agtcatcggt
    23101 ttgattgtgg gtgcgctggc gaaagcaatt atgccgggta aagaccccgg cggcataatc
    23161 gtcacaatcc tgatcggtat tgtgggcagc attatcggcc attacatcgc cgccgctatt
    23221 tatacgagca cagagggacc gggctttatc atgcggcttg tcttcgcgat tatcgggtcg
    23281 ctcattctgc tcggcggcta caggctcatc acagggcgca atagcacacg tgtctgacgg
    23341 gtagcgcaag gtctaattct ttcgatcttt gtaaaaatgg gcgcggttct gagttgagat
    23401 gagtcagagc cgcgtctatt tttcgtcatt tcctcttaaa ctacggtaaa caaaagggat
    23461 tacagaaaat agagccttcc atgcagctat aatccggcgg tcggccaatt gtcacaaagt
    23521 ccttcctaaa accctcaagt ttagacttca accgatgcga cgttttttca acccgacgat
    23581 cttttccaca cctgtgaacg cacgcgtcgc cccctatcta actgttagat aaagcagatt
    23641 tcgagtcctg cccacgcggc ggatttccgt tcgccgcctg ttcgcccttt taagcgctta
    23701 agacctgtta cttcaagtaa ctgttgaact tagccgtata aaccgttgta aagaaatgtc
    23761 tttacagcat attacccgca tgctaaattc ccgccacttt gtcgctaaac gtttgagcga
    23821 taaagaattg aacgtgatca agccacactc cgttgtagcg ctccagattt gaagacacac
    23881 tatcttgagt tctcgacata aattgacata gacaggacac agaaacccag accgaaatgc
    23941 ctatcaggtt cggaacatca ggctggcgcg caatcatcgc agacgaattc actttcgcca
    24001 acgtgcggct cgtaactcgc gctatttgca gctacttacg cacgaacggc gtcgaggacg
    24061 gcgcaagtct cgtcgtcagt tacgacacgc gttttctggg agagaagttt gcggctcagt
    24121 gcgtggaaga aatagtcgat aacgggctta aggcaatatt ttgcgaccag ccgacgccga
    24181 cgccgaccat ctctcatacg atccgcgaac gcggcgcggc aggtggaatt aacttcacgg
    24241 catcgcataa ccctcctgaa tacaacggga tgaagttttc gaccgctgac ggcgcgcccg
    24301 ccctgcccga cgccaccagg caggtcgagg ggctgatcgg gagcatgggg gcggcgagcg
    24361 aaagcaggtc gcgcgacaaa aacaccgccg atattgaagt ttacgacccg cgcccggcct
    24421 atttcgcaga ccttgaacgg aaactgcgtt tcgacgtaat tgccggagcc ggtgggactt
    24481 atgcgttcga cccgttatgg ggcacggggc gcggctatct ggatcgtgcc ttacgcgagc
    24541 acggcctcga agtagaaacg attcacgatt ggcgcgatgt gatgtttggc gggcgttcgc
    24601 cggagccgga cgatcatcat ctggacgagt tgcgcgaggc gatgaaacgg ggcgggtgcg
    24661 tgctcggcct cgccacggac ggcgacggag atcgtttcgg cgtgctcgac gcagacggaa
    24721 ctttcatcac gcccaaccgg ctcatcccgc ttctgttcga ctacctcgcc gaatcgcgcg
    24781 ggtggacagg cggcgtcgcg cgttcggtcg ccacttcgca tctcgtggat cgcgtcgccg
    24841 aatcgcgcgg tttgccggtc tatgaaacgc cggtcggctt caagtttatc ggcgagttga
    24901 tcaacgagga caagatcatc ctcggcggcg aagagtcggc aggactctcg attcgcggcc
    24961 actacccgga gaaggacggc attctggctt gcctgttggc agccgaagcg gtggcggcgc
    25021 gcggcgtgag tctgaacgag cagttgaatg cgttgtacgc gcgagtcggg cgacttgagg
    25081 caggacgcat cggcgtacgt ttaacgccgg agattttggc gacgctgccc gagaaattaa
    25141 gacgcgaccc cacagacatc ggcgggcggc gcgtggctcg cacggatcgc atggacggag
    25201 tgaagtttat ttttgcagat ggcgcgtggc tgctgctgcg tccttcgggg acggagccgc
    25261 tcgtgcgcat ctacgctgaa tctgaatcgg agaaagagtt ggaggtgctt cttgaacaag
    25321 gccgcaaata cttattggac tgactcgcgc ctcgccgcgc aacgccctac ggcacgacct
    25381 ctcgcgctgg cgaccccggc gcgcgaactc tcacgcgaac tcgtcgggac gcgcgcagag
    25441 gtacgccgtc gtggtggact tattccctca tgggctgtct tcacgatgat catgctcgcc
    25501 accttcgcgc tgtgcgtcag cgtcacgatg cggacgcatg cggagatgcg cagtgcggag
    25561 cagaaacttt cacagatgaa tacggaggtg caggcaatca gcaatacgaa cgactcgctc
    25621 aagcaagagg tgcagcgttt gcaggagcgt aagtcgcgcg ccatcgagga cgccgcacgc
    25681 gcacggctca atatgattcg tccgaacgag atcgttgtgc ccattgatta accgacaagt
    25741 ggattaaccg acaaggagga ggctctttat cgcagtgaac tgtgtcgcac ttataccaag
    25801 ttctgccggt cgcccgccgc tcgctgcccg cttagtttct gcttagcgtt agctcctgcc
    25861 tgacaccgcg cggctgcctg tttaaagcag gtagccgcgc tacttcatct agagattccc
    25921 ctccattttg tggccggaac atgcggttga cacccccgca gtggtcaact aatatcaaac
    25981 tctatttaac tcgtcataaa ttttattgac ctcgaaaccg cctagttttc gagcgggaga
    26041 gacaagcaaa atcatgtccg aag
//
LOCUS       DASWNS010000619         2962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_101061, whole genome shotgun sequence.
ACCESSION   DASWNS010000619 DASWNS010000000
VERSION     DASWNS010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101061"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1156
                     /locus_tag="VGB76_21410"
     CDS             179..1156
                     /locus_tag="VGB76_21410"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY0101498.1"
                     /translation="MPRALLLLAAVLAVSTFVALWVGYAPIAFADLRGDEMARLLFFR
                     LRLPRVLMAGMVGASLAMVGASLQALFRNPLAEPLTLGVSGGGALGASIAIAFGFGAR
                     VVGVPVIFLAAFAGAGAAMLVVYRLARTGAVVMPGALLLAGVVLNFVAASFVVIIQYF
                     TDYSRALQILRWTIGSLDVVGFDLIWRMLLFLVPGWIVLALMARDLHLIALDEETAAS
                     LGVNVRRSERLVYAASSMIVGVTVAVGGTIGFVGLIVPHAVRLLFGEDVRVILPCSFL
                     LGAAFLMLADAVARTVLGVGELPVGAITALAGGPVFLWLLRRQQRLAAM"
     gene            1296..1871
                     /locus_tag="VGB76_21415"
     CDS             1296..1871
                     /locus_tag="VGB76_21415"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY0101499.1"
                     /translation="MSIATKAGDKGDTGLIGGQRVSKADLRVEAYGTIDELGSTLGFA
                     RAICDDAEVRALTKEIQRELFAVSGGVANPLGAQKEPPYVTEAMVGVLTAEVNRIEGM
                     EGILSDWSLPGEHAAAAAYDVARTVCRRAERAVVRLTEAGEEVSPQTVAYLNRLSDLL
                     WLMGRLLELRAGVDTRLRDDAHTGPRWSRAW"
     gene            complement(2009..2422)
                     /locus_tag="VGB76_21420"
     CDS             complement(2009..2422)
                     /locus_tag="VGB76_21420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY0101500.1"
                     /translation="MEIIKVRSREREELVEFTDEVRRRLKATGAREGICVLYVQHTTA
                     GLTINENADPDVPRDMLHALRTLIPQHGMNFRHNEENSDAHIKATLTGSSLTIPFHDG
                     ELLLGRWQGIFLCEFDGARERQIIFQVMNAKNSDK"
     gene            complement(2482..>2962)
                     /locus_tag="VGB76_21425"
     CDS             complement(2482..>2962)
                     /locus_tag="VGB76_21425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0101501.1"
                     /translation="AKSIQLVARFARNVGMVSGDPDRLQQVVWNLLSNAVKFTPVGGR
                     VEVSLKRAGAHEAELVVSDNGTGISQDFLPHVFEHFRQADGTSTRSHGGLGLGLAIVR
                     RLIELHGGTVKAESAGEGHGATFRIRLPLLRRASERKQASERKRANSAGTRRKSARG"
BASE COUNT          470 a          853 c         1003 g          636 t
ORIGIN      
        1 atgctttctt tcatcctttc ttcccggtgc gcttcctgcg tctgcggcgt tgaaggtttt
       61 taaccgcgca cgccacgagt tgctttaaaa gcgtaaacac taagcgacag ttagaaaaca
      121 caagttgact cgaaaccgga aagcaaaagt tgtaaagcaa aaatctgcgg cggggcgttt
      181 gccgcgcgcg ttgttgctgc tggcagcggt gctcgccgtc tcgacctttg ttgcgctgtg
      241 ggtcgggtat gcgccgattg cgttcgcgga cttgcgcggg gatgagatgg cgcggctgct
      301 cttttttcgt ttgcgcctgc cgcgcgtgtt gatggcgggg atggtggggg cttcgctggc
      361 gatggtgggg gcgtcgttgc aggctctgtt tcgcaacccg ctcgcggagc ctctgacgct
      421 cggcgtgtcg ggcggcggcg cgctcggcgc gagcatcgcg attgcgttcg gcttcggcgc
      481 gcgggtggtg ggcgtcccgg tcatcttcct cgcggcgttt gcgggcgcgg gcgcggcaat
      541 gctcgtcgtc tatcgtctcg cgcgcacggg ggcggtcgtg atgccgggcg cgctgctgct
      601 cgcgggcgtc gtgctcaatt tcgtcgcggc ctctttcgtc gtcatcattc aatacttcac
      661 ggactattca cgcgcgctgc aaatcctgcg ctggactatc ggcagcctcg acgtggtggg
      721 cttcgatctc atctggcgca tgctgctctt tttagtgccc ggctggatcg tgctcgccct
      781 gatggcgcgt gacctgcacc tgatcgcgct cgacgaagag acggcggcga gcctcggcgt
      841 caacgtgcgc cggagcgagc gactggtgta cgcggcgagt tctatgatcg tcggcgtgac
      901 ggtggcggtc ggcggcacga ttggcttcgt cggcctgatc gtgcctcatg ccgtgcgcct
      961 gctctttggc gaagacgtgc gcgtgatcct gccctgctcg tttctgctcg gcgcggcgtt
     1021 cctgatgctg gccgatgcgg tcgcgcgcac ggtgttgggc gtaggcgaac tccccgtcgg
     1081 cgcgatcacc gcgctcgcgg gaggccccgt ctttttatgg ctgctcagac ggcagcaacg
     1141 tctggcggcg atgtgaaaat tgtcagcggt caacggtcga gtgtcagtcg tccgttgttt
     1201 gtgtagcgcg tgcgagtcgc cgaagattcg gattgagcaa ctgacaactg actaccggca
     1261 actgactacc ggcaacggac atttcgggag gctttatgag catagcgacg aaggcgggcg
     1321 acaagggcga caccggactc atcggcggcc agcgcgtttc gaaggccgac ttgagggttg
     1381 aggcttacgg gacgattgac gagttggggt cgacgctcgg gttcgcgcgt gcgatctgcg
     1441 acgacgcgga ggtgcgcgct ttgacgaagg agattcagcg cgagttgttc gcagtgagcg
     1501 gcggcgtagc gaatcctctg ggcgcgcaaa aagagcctcc gtatgtgacg gaggcgatgg
     1561 tcggagtatt gaccgcagag gtcaaccgca tcgaggggat ggaagggatt ttgtccgact
     1621 ggtcgctgcc gggcgagcac gcggcggcgg cggcttacga tgtggcgcgc accgtctgcc
     1681 gccgcgctga acgcgcggtg gtgcgcctca cggaagcggg cgaagaagtc tcgccgcaga
     1741 cggtggccta cctcaatcgc ctctccgacc tgctctggct gatgggtcgc ctgctcgaac
     1801 tgcgcgcggg cgtggacacg cgcctgcgcg acgacgcaca cacgggacca cgctggtcgc
     1861 gggcatggta aagcagtgac gagtgacaag cgacgagtga cgagagaaga caggtaaatc
     1921 gtgaatcgtc aatcgtgaat agaggaaaaa ctggctttta ctattgacga ttgacgattg
     1981 acgattcacg gctttttctc gtcatctgtc acttgtcact gttcttagca ttcatcactt
     2041 gaaagatgat ttggcgttcg cgtgcgccgt cgaactcgca gaggaagata ccttgccagc
     2101 gtcctaagag taattcgccg tcgtggaagg ggatggttag gcttgagcct gtgagcgtgg
     2161 ctttgatgtg cgcgtcggag ttctcttcgt tgtggcgaaa gttcatgccg tgttgcggga
     2221 tgagggtgcg caaggcatgg agcatgtcgc gcggcacgtc cgggtcggcg ttctcgttga
     2281 tggtcagacc ggcggtggtg tgctgcacgt agagcacgca gatgccttcg cgcgcgcccg
     2341 tcgccttgag cctccggcgc acttcgtccg tgaactcgac caactcttcg cgctcgcgcg
     2401 aacgaacttt gatgatctcc atagtcgccc gtcttgccgt aaataagagt tgaaaatttt
     2461 cgccctgagc gggaaagcga ctcagccgcg cgcgctcttc cggcgcgtgc ccgcgctgtt
     2521 cgcccgcttc cgctcgcttg cctgcttccg ttcgctcgcg cgccggagca gcggcaaccg
     2581 gatgcgaaaa gttgcgccgt gtccttcgcc cgcgctttcg gctttcactg tgccgccgtg
     2641 aagttcgatc aagcggcgca cgatggcgag tccgagtccg aggccgccat gcgagcgggt
     2701 gctggtgccg tcggcctgcc ggaagtgttc aaagacgtgc ggcaggaaat cctgactgat
     2761 ccccgtgccg ttatccgaga cgaccagttc ggcctcgtgc gcgcccgcgc gtttgagcga
     2821 gacttccacg cgccctccga cgggcgtaaa cttcaccgcg ttcgaaagca gattccacac
     2881 cacctgctgc aagcgatccg ggtcgcccga aaccatgccg acgttccgtg cgaagcgcgc
     2941 gacgagttgg atcgacttgg ct
//
LOCUS       DASWNS010000620        10959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_232963, whole genome shotgun sequence.
ACCESSION   DASWNS010000620 DASWNS010000000
VERSION     DASWNS010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10959
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232963"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2558)
                     /locus_tag="VGB76_21430"
     CDS             complement(<1..2558)
                     /locus_tag="VGB76_21430"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEY0101502.1"
                     /translation="MKSARLKSTHTSTHRHLINTFVILLLLCALSLLVAIVAPVRQSA
                     PPDAAAGMNSSTRLPTSTTTPATEPVAERAREAYGRMELSFEANRGQTAAPVNFLARG
                     AGYTLFLKPAEAVFVMTPQGGRRKAHHDVSRERTQAIEDVNINTTARSNNTAKRARQH
                     IEPEPQTTFSMKLLGARADAPVESADELAGKVNYFYGNDPAKWRTDIPTFGRVRYKEV
                     YPGIDVVYYGNQRQLEYDFVVAPGSDARAVSLEFAGADKLEVDEGGDLLLTVGKEVVR
                     QLKPVIYQETAGGARRAVEGGYVLQAGGRVGFRVGEYDASRPLVVDPVLVYSTYLGGN
                     STDAAGTIAVDSSGNAYVAGTTGSTNFPTANPLQPALSDVANVFVTKINAAGTALVYS
                     TYLGGNSSEQATGIALDSSGNVYITGFTGSTTFPTVNPIKGARGCCHEDAFVAKLNAS
                     GSALVYSTYLGGNASADFAEDIALDSSGNVYVTGRTHANDFPLVNPIQGTLRGFSDVF
                     VTKINAAGSAFVYSTYLGGSLGDGGAGIVVDASDNAYVTGTAESPNFPLANPLQRTYN
                     GGSSDAFVAKLNAAGSALIYSTYLGGGDDERSFDIAIDSSGNAYAAGDTLSTDFPVAN
                     AIQSTKGGKVGTYDAFVTKINASGSALVYSTYLGGLGNEFAFSIAVDAAGSAHVTGRI
                     DSFNSFPLLNAIQCTRKGFEDMFITKFNAAGSALVYSTYLGGTEVEAGRDIALDSSGN
                     VYLVGGTTSVDFPTVNPIQSTFGSHNQFAPDAFITKLNNEPTPNPCTPAPPPPTPTPP
                     PPPTPTPSPTPCPVVIIYTVSNAVLGFGADTIKLSGGSRIMQLTSGTTHSDLFYG"
     gene            complement(2899..3309)
                     /locus_tag="VGB76_21435"
     CDS             complement(2899..3309)
                     /locus_tag="VGB76_21435"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0101503.1"
                     /translation="MKRKKGDRTYTISAVAEQYGLHQQTLRLYEREGLIKPSRSDGNT
                     RLYTDADLERLEVILSLTRDLGVNLAGVEIILNMREKMDAMQREFERFFAYLQSHAEE
                     FAAFTDAPPPDAGALVPVRRANILLQRRRTTSRG"
     gene            complement(3415..4620)
                     /gene="dnaJ"
                     /locus_tag="VGB76_21440"
     CDS             complement(3415..4620)
                     /gene="dnaJ"
                     /locus_tag="VGB76_21440"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY0101504.1"
                     /translation="MAKEDFYKVLGVERKAKPEEIKKAYRRLARKHHPDVNPGDKAAE
                     ERFKLITEAHDVLSDPKKRSVYDRFGQYSDNLADAAARGAEPGGGAGGRSAPFDFTGF
                     DWSTGTAAGGSSGGGGNAGGSSSFRDIFADLFGGGGGAGGTKAEREPPRPMPQRGADI
                     EMPLALSFEEAVTGMTTNITVTRSEQCTRCNGAGDTGGAPVTCPTCKGAGQVQRAGGR
                     LRFAQECPDCDGAGKRRPPCSLCQGKGVLPKTESVKVRIPAGVDTGSRVRIPGKGEGG
                     RLGAPPGDLFIITNVGRHKHFTRKGDNIYVTIPVTVPEAALGAKIEVPTVEGKAQLRI
                     PPGTQSGQKFRLRERGAPSLRNPQARGDQFVEVQVTFPKVISEETKELLQRYAQLNAE
                     NPRVAMGLE"
     gene            complement(4795..6765)
                     /gene="dnaK"
                     /locus_tag="VGB76_21445"
     CDS             complement(4795..6765)
                     /gene="dnaK"
                     /locus_tag="VGB76_21445"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY0101505.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGEPTVITNSEGGRTTPSVVGFAK
                     DGNRLVGQVAKRQAVTNPENTIYSIKRFMGRGFDEVTEEKKQVPYQVQAAGNRDVRIV
                     AGGKDYSPPEISAMILQKMKQSAEDYLGQKVDKAVITVPAYFNDAQRQATKDAGKIAG
                     LEVMRIVNEPTAAALAYGLDKKKEEVIAVFDFGGGTFDISILEVGEGVVEVKSTNGDT
                     HLGGDDIDERLMQWITEEFKKDQGIDLSNDKMALQRLKEAAEKAKIELSTAMETEVNL
                     PFITADASGPKHLVMKLTRSKFEQLIDPILQRLKKPVEEAIADAKKAIKDSNFDATKV
                     NEVVLVGGSTRIPKVQEIVKSIFGKEPNKSVNPDEVVAIGAAVQAGVLSGEKTDILLL
                     DVTPLSLGIETLGGVFTKLIERNTTIPTRKSEVFSTASDNQTSVEVHVLQGERQMAGD
                     NRTLGKFHLVGIPPAPRGMPQVEVTFDIDANGIVKVSAMDLGTKRVQDITITASSGLS
                     KDEIDRMMREAESHAGEDAKKREEIEARNRLDGLTYQVEKTFNENKEKLDSASTTQLE
                     TALSDAKKALEEGGADRLNDAYKNLETASHKLAEALYQQTAQAGGGGEAGGEQASAAG
                     ASASGAQGGTASGGGGDDNVIDAEYVDVDENK"
     gene            complement(6993..7595)
                     /locus_tag="VGB76_21450"
     CDS             complement(6993..7595)
                     /locus_tag="VGB76_21450"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0101506.1"
                     /translation="MSSRIFTVPNFLTVIRMVLVPVFVTMLYYQRFDWALAIFIFAGL
                     TDGIDGFLARRFNQKSQLGTILDPIADKLLLVTSFITLSLASIVGRGEHLPVPFWVTA
                     AVISRDVFIIVGAAAINIVTGFRGFRPSLLGKVNTVVQITAIVIILALARFPSLRGYI
                     LPTVYVIVFASALFSGMHYIFHVSRIMNEDRHEREMTENP"
     gene            complement(7768..8628)
                     /locus_tag="VGB76_21455"
     CDS             complement(7768..8628)
                     /locus_tag="VGB76_21455"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0101507.1"
                     /translation="MPNIARWKSPRRPLDARPKPLRTLAAGVMQMARAAFAEPYKLVV
                     ERSTINLRRLPDALDGLRIVQLSDIHHSPFTGRAQVERAVELANSLQPDIIALTGDYV
                     SHEREYAAPCAEMLGRLRARCGVFAVLGNHDHWTDAALITDLFRAEGIRVLVNEGLRF
                     EHPKHPGATFWLAGVDDTMVGLEDLPLALAGSSADEMKLLLAHNPIILRRAARAGVDL
                     VLSGHTHGGQVTLRAERSASGRPRRRLLRGLGGQNDTQIYVTRGLGTVVLPVRYGCLP
                     EVSLLELRKQ"
     gene            complement(8825..10609)
                     /locus_tag="VGB76_21460"
     CDS             complement(8825..10609)
                     /locus_tag="VGB76_21460"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY0101508.1"
                     /translation="MTLSIATVLILLFVALVLFGTERIPIDVVTILVVIGLVLTGVLT
                     PAEGFAGFGNDIIITISGLFILTGGLVKTGVVDLVGRRLHRVSGGSEFRLTVLIMFTA
                     AACASVMKNTTTTAMFVPVVLGLAERAKVQPSKLLMPLAFGAILGGTCTLIGTSTNLA
                     VSGAMTRYNLAPFTMFELTPVGIAIVVVGMLYMLLVGLRLLPARGSADSLADAYHIRE
                     YMSEVVVLDTSPLVGQTLAEANLSQSLDLAVVGILRGKQGRIAPRADEQILAGDLLLV
                     QGRIEDILRVKTEAGIEIKADFKLSDTDLEAGNLELFEAMVLRGSNLVGRTLKGTKFR
                     ERYGLTALAINRHGVALLSKLSMVSLRFGDVLLVQGKRAQVEHLATEGNLLLLEDVSE
                     RRGRAAKRRWALLAFAVFLIFSIFHPFNVPLSVAVLFGVLILLASRAVLSGEIYGMID
                     FRLIVLIAGMISFGTAMEKTGADDYLAGLIVNSVGGFGPYAVMGGFILLTIGLTQPMS
                     NQAAALVVLPVAIQTATQLGLNPRAFAVAVTYAASCSFLTPLEPACVLIYTPGRYRFF
                     DFVKIGSLLTIAVFIIIMWLVPMRWPLS"
     gene            10717..>10959
                     /locus_tag="VGB76_21465"
     CDS             10717..>10959
                     /locus_tag="VGB76_21465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0101509.1"
                     /translation="MSKHRILVIEDQEDLAELYESSLEKAGYEVTNAYTGEEGVAEFE
                     ANGADAVLLDMTLPEMHGTKVLEEIRNLSPSVPVVVV"
BASE COUNT         2059 a         3187 c         3484 g         2229 t
ORIGIN      
        1 ccgtaaaaca gatcgctgtg cgttgtgccg gaggtgagct gcatgatgcg gctccctccg
       61 ctaagtttaa tcgtatctgc gccgaaacct aacacggcgt tgctcaccgt gtaaatgatt
      121 accaccggac acggcgtcgg gcttggggtc ggagttggcg gcggcggcgg cgtcggcgtc
      181 ggcggtggcg gcgccggggt gcaggggttc ggcgtgggtt cgttgttgag tttggtgatg
      241 aaggcgtcgg gggcgaattg gttatggctg ccgaacgtgc tctggatggg gttgacggtc
      301 gggaagtcaa ccgaagtagt tccaccaacg agatagacgt taccggatga gtcgagcgcg
      361 atgtcccgtc cggcttcgac ttcggtgccg ccgagataag tcgagtaaac taacgccgac
      421 cccgccgcgt tgaacttcgt gatgaacata tcttcgaagc ccttacgtgt gcattgtatg
      481 gcgttgagca gggggaaact gttgaatgag tcaatgcgtc ctgtgacatg tgcgctgcct
      541 gcggcgtcaa ccgcgatgct gaaagcgaac tcgttaccca agccgccgag ataagtcgag
      601 tacacgagcg cggaacctga ggcgttgatc ttcgtcacga aggcgtcgta agtgccgacc
      661 ttgccgcctt tcgtgctttg gatggcattg gcgacgggga aatcggttga cagcgtgtcg
      721 cctgcggcgt aagcgttgcc cgacgaatca atggcgatgt cgaagctgcg ttcgtcgtcg
      781 ccgccgccga gataagtgga atagatgagc gcggaaccgg cggcattgag tttagcgacg
      841 aaagcgtcgg atgagccgcc gttgtacgtg cgttgcagcg gattagcgag ggggaagttg
      901 ggcgattcgg cagttccggt gacgtaagcg ttgtccgaag catctacgac gatgcccgcg
      961 cctccgtcac ccagactgcc gccgagataa gttgagtaaa cgaaggccga ccccgccgcg
     1021 ttgatcttcg tcacgaagac atcactaaag ccgcggagcg tgccttgaat ggggttgacg
     1081 agggggaagt cgttcgcatg ggtgcgaccg gtgacgtaga cattccccga agaatcaagc
     1141 gcgatgtcct cggcaaagtc tgcactcgcg ttgccgccga gataagttga gtaaacgagc
     1201 gcggaacctg aagcgttgag cttcgccacg aaggcgtcct cgtggcagca gcctctcgcg
     1261 cctttaatag gattgacggt gggaaaggtg gttgacccgg tgaagcctgt gatgtaaacg
     1321 ttgcccgacg aatcaagcgc gatgcctgta gcctgctcgc tactgttgcc gccgaggtag
     1381 gtggaataga cgagcgccgt gcccgccgcg ttgatcttcg tcacaaagac atttgccacg
     1441 tcgctcagcg cgggttggag gggattagcc gtcgggaaat tagtcgaacc tgtggtgcct
     1501 gcgacgtaag cgttgccgga cgagtccacc gcgatggttc ccgcggcgtc cgtgctgttg
     1561 ccgccgagat aagtggaata gacgagaacc ggatcaacga cgagcggacg cgacgcgtcg
     1621 tactcgccga cgcggaagcc gacgcgcccg cccgcttgca acacatatcc accttcgacc
     1681 gcgcgccgcg cgccgcccgc cgtttcctga tagatcacag gcttcagttg ccgcacgact
     1741 tctttgccca ccgtcagcag caaatcgccg ccctcgtcca cttccaactt gtccgcgccc
     1801 gcgaactcaa ggctcacggc acgcgcgtcg cttccgggcg cgaccacgaa gtcgtattcc
     1861 aactgccgct ggttgccgta atagaccacg tcaatgcccg gatagacttc cttgtagcgc
     1921 acgcgcccga aggtcggaat atccgtgcgc catttggccg gatcgttgcc gtagaaatag
     1981 ttgaccttgc ccgccaactc gtccgcgctc tcgaccggcg cgtccgcgcg tgcgcccaag
     2041 agtttcatgc tgaacgtcgt ctgcggctcc ggctcaatgt gttggcgagc gcgcttcgca
     2101 gtgttgttgc tgcgcgccgt ggtgttgatg ttcacatcct caatcgcctg tgtccgctcg
     2161 cggctcacat catggtgcgc ctttcgcctc ccgccctgtg gcgtcatgac gaagacggct
     2221 tctgcgggtt tgagaaagag cgtgtagccc gcgccccgcg cgaggaagtt gacgggcgca
     2281 gcggtctgcc cacggttggc ttcaaagctc aactccatcc gcccgtatgc ctcgcgcgca
     2341 cgctcggcca ctggctcggt cgctggcgtt gtagttgatg taggcaaccg tgtcgaagag
     2401 ttcataccgg cggcagcatc cggcggtgcg ctctgcctga caggcgcgac gattgcgacg
     2461 agcaagctca aggcgcatag cagcagcagg ataacgaacg tgttgatgag gtgtcggtga
     2521 gtagaggtgt gagtagattt caagcgggca gatttcatgg tgtcctcgtc ttcggttgtg
     2581 gcgtgtggtt ggcaacgccc tacgactttg agcggcggca ggtctgcgcg gtgtgtgcgc
     2641 aagcagttgg cggtttaggc agggtcaggt tgacgcctct gcttctttgg cccccggttt
     2701 tggactgtgg agcgcatcct ttcacagttc gtcccgtttg agcaagcgat tctgagcaag
     2761 cgatattttg agttgttgct tgtagtgaga cgggatgatg gcgagaatca aagcgcgggg
     2821 atatttgaag cgacggggag cagaggagcg cgtcagaccc gcccgccttc gcaagcggtt
     2881 ctgacgtctt ttgacttttt aaccgcgcga agtggtgcgg cggcgttgga gcaggatgtt
     2941 tgcgcggcgc acggggacga gcgcgcccgc gtcgggcggc ggcgcgtccg tgaaggccgc
     3001 aaactcttcg gcgtgcgatt gcaggtaagc gaagaagcgt tcgaactcgc gctgcatcgc
     3061 gtccatcttt tcccgcatgt tcaggatgat ctcgacgccc gcgagattaa cgccgagatc
     3121 gcgcgtcaga gacaggatca cttcgaggcg ttcgaggtcg gcatccgtgt agagccgcgt
     3181 gttgccgtcc gagcgggaag gcttaatcag cccctcgcgc tcgtagagac gcaaagtctg
     3241 ctggtgcagc ccgtactgct cggccaccgc gctgatcgta taggtgcgat cacctttttt
     3301 gcgtttcata agaattcagt gacgagtgac gagtgacaag tgccgagccg gaggacaagt
     3361 taaagacaag tatgctgctt taacttgtca ctcgtcattt gtcactcgtc actgctattc
     3421 aagtcccatc gcgacgcgcg ggttctcggc gttgagttgc gcgtagcgtt ggagcagttc
     3481 cttagtctct tccgagatca ctttggggaa cgtgacctgc acttccacaa actggtcgcc
     3541 gcgcgcctgc gggttacgca gggagggcgc gccgcgttcg cgcaggcgaa acttctggcc
     3601 tgactgcgtg cccggcggaa tcctgagttg cgctttgcct tccaccgtcg gcacttcgat
     3661 cttcgcgccg agcgcggctt ccggcaccgt gacgggaatc gtcacgtaga tgttgtcgcc
     3721 cttgcgcgtg aagtgcttgt gccgcccgac gttcgtgatg atgaagaggt cgcccggcgg
     3781 cgcaccgagc cgcccgcctt cgcctttgcc cggaatacgc acgcgcgaac ccgtatccac
     3841 gcccgccggg atacgcacct tcacgctctc cgtcttcggc aaaactccct tgccctgaca
     3901 cagcgaacac ggaggccgac gcttgcccgc cccgtcgcag tccgggcact cctgcgcaaa
     3961 cctgagccga ccgcccgcgc gttgcacttg ccctgcgccc ttgcaggtcg ggcaggtgac
     4021 gggcgcgccg cccgtgtcgc ccgcgccgtt gcagcgcgta cactgctcgg aacgtgtgac
     4081 cgtgatgttc gtcgtcatgc ccgtcacggc ttcttcaaac gagagcgcga gcggcatctc
     4141 gatgtccgcg ccgcgctgcg gcatagggcg cggcggttcg cgttcggcct tcgtcccgcc
     4201 tgctccgccc ccgccgccga acaggtctgc gaagatgtct cgaaaactcg aactccctcc
     4261 ggcgtttccg ccgccgccac tgctgccgcc cgccgccgtg ccggtgctcc agtcgaaacc
     4321 tgtgaagtcg aagggcgcgc tgcgacctcc cgcgccgccg ccgggttccg caccgcgcgc
     4381 cgccgcgtcc gccagattgt ccgagtattg cccgaaccga tcatagacgc tgcgcttctt
     4441 cggatcggag agcacgtcgt gcgcctctgt gatcaacttg aagcgttcct cggcggcctt
     4501 atcgcccgga ttcacgtccg ggtggtgctt gcgcgccagc cgcctgtacg ctttcttgat
     4561 ctcttcgggc ttcgccttgc gctccacgcc cagcactttg tagaagtctt cttttgccat
     4621 tctggagaca gtgacaagtg acaagtgaca agtgacgagt taaggcaagt gaatcgtaaa
     4681 tcgtgaatcg caaatcgtaa atagaaagag aactgccttc ctccattcac gattgacgat
     4741 tcacgattta catcttttca acccgtcacc cgtcacccgt cactcatcac tgttttactt
     4801 gttttcgtct acgtcaacgt attccgcgtc gataacgttg tcgtcaccgc cgccgccgga
     4861 ggccgtgccg ccctgtgcgc cggaggccga cgcgcccgcg gcggaagcct gttcaccgcc
     4921 cgcttcgccg ccgccgccag cctgcgcagt ctgctggtag agagcttcgg cgagtttgtg
     4981 cgaggcggtt tcgaggttct tgtaagcgtc gttcaggcga tccgctccgc cctcttcgag
     5041 agcctttttg gcatcggaga gcgcggtttc gagttgcgtc gtggacgcgc tatcgagctt
     5101 ctccttgttc tcgttgaagg tcttctccac ctgatacgtc aaaccgtcga gacgattacg
     5161 cgcttcgatc tcctcgcgct tcttcgcgtc ttcgcccgcg tgcgattcgg cctcacgcat
     5221 catgcggtcg atctcatctt tcgacagacc ggacgaggcc gtgatggtga tgtcctgcac
     5281 acgcttcgtg ccgaggtcca tcgcagacac tttgacgatg ccgttggcat cgatgtcaaa
     5341 cgtcacttcc acctgcggca tgccgcgcgg cgcgggcgga atcccgacca gatgaaactt
     5401 gccgagcgtg cggttgtcgc ccgccatctg gcgttcgcct tgcagcacgt gaacttccac
     5461 ggaagtctgg ttgtcggaag cggtcgaaaa gacctcggac ttccgcgtcg ggatcgtcgt
     5521 gttgcgctcg atcagtttcg tgaagacgcc gccgagcgtt tcgatcccta agctgagcgg
     5581 tgtcacgtcg agcaacagga tgtccgtctt ctcgcctgag aggacgcccg cctgcaccgc
     5641 cgcgccgatg gcgaccactt catccgggtt cacgctcttg ttcggctctt tgccgaaaat
     5701 gctcttgacg atctcctgca ctttcgggat gcgcgtcgaa ccgccgacca agaccacttc
     5761 gttcactttg gtcgcgtcga aattggagtc cttgatcgcc ttcttcgcgt cggcgatggc
     5821 ctcttccaca ggcttcttca gacgttgcag gatcggatcg atcaactgct cgaacttcga
     5881 gcgcgtcaac ttcatcacga gatgcttcgg ccccgaagcg tcagccgtga tgaacggcag
     5941 gttcacttcc gtctccatcg cggtcgagag ttcgatcttc gctttctcgg ctgcttcctt
     6001 cagacgctgc aaagccatct tgtcgttcga aagatcaatg ccctgatcct ttttgaactc
     6061 ttccgtgatc cactgcatca ggcgttcgtc tatgtcgtcg ccgccgaggt gcgtgtcgcc
     6121 gttcgtggat ttcacttcga cgacgccttc gccgacttcg aggatcgaga tgtcgaacgt
     6181 gccgccgccg aagtcgaaga cggcgatgac ctcttccttc ttcttgtcga ggccgtaagc
     6241 cagagccgcc gccgtcggct cgttgacgat gcgcatcact tcgaggccgg cgatcttccc
     6301 tgcgtctttc gtggcctgcc gctgcgcgtc gttgaagtag gccgggacgg tgatgacggc
     6361 cttgtcaact ttctggccga gatagtcttc cgccgactgc ttcatctttt gcaggatcat
     6421 cgcggaaatt tcgggcgggc tgtagtcctt gcccccggcg acgatgcgca cgtcgcggtt
     6481 tccggcggcc tgcacctgat aaggaacctg ttttttctct tcggtcactt cgtcgaagcc
     6541 gcgtcccatg aaacgtttga tcgaatagat cgtgttctcc gggttggtga cggcctgacg
     6601 cttcgccacc tgaccgacca gacggttgcc gtctttcgcg aagccgacga cggaaggcgt
     6661 cgtgcgcccg ccctcggagt ttgtgatgac ggtcggctcg ccgccttcca tcacggccac
     6721 cacggagttc gtcgtgccca agtcaatacc gattatcttg ctcatcgttt tcactctcca
     6781 gtgttattta gctacccgcc tctgactcac gcgtccggct cgaacggcca caaaccgtct
     6841 cacggactta actcaatcgc atctcagata taaaggcata ggttgaatac agcgttaata
     6901 taatatctga gtcccttctt gtcaactttt gattacagaa gcccgcgcag agttgtctcg
     6961 ttaagaccta aacccattaa aacccctgtt tatcaggggt tttcggtcat ctcgcgctcg
     7021 tgcctgtctt cgttcatgat gcgggagacg tggaaaatgt agtgcatgcc ggagaagagc
     7081 gcggaggcga agacgatgac gtagacggtt ggcaggatgt agccgcgcag agaggggaaa
     7141 cgcgccagcg cgaggatgat cacgatagcc gtaatttgca cgaccgtgtt gacttttccg
     7201 agcagcgagg ggcgaaaacc gcgaaaacct gtgacgatgt tgatggctgc cgcgcccacg
     7261 atgatgaaga catcgcggct gatgacggct gccgtcaccc agaaggggac gggcaggtgt
     7321 tcgccacgcc cgacgatgga ggcgagcgag agggtgatga aagaggtgac gagcaggagt
     7381 ttgtctgcga tggggtcgag gatggtgccg agttgcgatt tctggttgaa acggcgcgcg
     7441 agaaagccgt cgatgccgtc ggttaagccc gcgaagatga agatggcgag cgcccagtcg
     7501 aaacgctggt aatagagcat cgtgacgaag accggaacca gcaccatgcg gatgacggtc
     7561 aggaaattcg gcacggtgaa gatgcgagaa gacataagga cagtgacgcg tgacgggtga
     7621 cgcgtgacaa gcaagaagga agacaagtga atcgtgaatc gtggatcgta gatcgtgaat
     7681 agaagaaccg aagacgggtt tctctattta cgattcacga ttcacgattt acgtcttttc
     7741 aacttgtcac ctgtcacttg tcacttgtca ctgttttctg agttcgagga gagagacttc
     7801 gggcaagcag ccgtaacgca cgggcagaac taccgtgccg aggccgcgtg taacgtaaat
     7861 ttgcgtgtcg ttctggcctc ccaagccccg caggaggcgt cggcgggggc gacctgaagc
     7921 cgaacgctcc gcgcgcagcg tgacttgccc gccgtgcgtg tggccgctca ggacgagatc
     7981 aaccccggcg cgtgcggcgc gacgcaggat gatcgggtta tgcgcgagca ggagtttcat
     8041 ctcgtccgcg ctcgaaccgg cgagggcgag cgggaggtct tcgaggccga ccatcgtatc
     8101 gtccacgccc gcgagccaga aggtcgcgcc cgggtgtttc gggtgctcga agcgcaggcc
     8161 ctcgttgacg aggacgcgta tgccttcggc gcggaagagg tctgtgatga gcgcggcgtc
     8221 cgtccagtgg tcgtggttgc cgagcacggc gaagacgccg caacgcgcac gcaggcgtcc
     8281 gagcatctcc gcgcacgggg cggcatattc gcgttcgtgc gaaacgtagt cgcccgtcag
     8341 ggcgatgatg tcgggttgga ggctgttagc cagttcgacg gcgcgttcaa cctgtgcgcg
     8401 cccggtgaaa gggctgtggt ggatgtctga aagctgtacg atgcggaggc cgtcgagcgc
     8461 gtcgggcagg cggcgcaggt tgatcgtgct gcgttcgacg acgagtttgt aaggctctgc
     8521 gaaggcggca cgcgccatct gcatgacgcc tgcggccagc gtgcgcagag gcttcgggcg
     8581 cgcatcgaga ggacgacggg gagacttcca tctagcgatg ttcggcatac gatgacaatt
     8641 gaaagtggat aatgtgaatt tactttgcga gagctaatcc ctgtttgaaa cgctcctgtt
     8701 agaagcccgc gaattatacg aaggcaaaag cgaaaaggca aaagcgaaac tcaaacgcag
     8761 agacgagtga cgggtgacaa gttttttagt tttaacttgt cactcgtcac tcgtctctgc
     8821 gttttcaact gagcggccag cgcatcggga cgagccacat gatgatgatg aagacggcga
     8881 tggtcaggag cgacccgatc ttgacgaagt cgaagaagcg atagcgtccg ggcgtgtaga
     8941 tgagcacgca ggcgggttcg agcggcgtca ggaaggagca ggaggcggcg taggtgacgg
     9001 cgacggcgaa ggcgcgcggg ttcaggccga gttgagtggc cgtctggatg gcgacgggca
     9061 ggacgacgag cgcggcggcc tgattcgaca tcggctgcgt tagcccgatg gtcagcagaa
     9121 taaaaccgcc catcacggcg taagggccga aaccgccgac cgagttgacg atcagccccg
     9181 cgagataatc gtccgcgcct gtcttctcca tcgccgtgcc gaagctgatc atgcctgcga
     9241 tgaggacgat caggcgaaag tcgatcatgc cgtatatctc gccggagagc acggcgcgcg
     9301 aggcgagcag gatgagcacg ccgaagagca ccgcgaccga gagcggcacg ttgaacgggt
     9361 ggaagatgga gaagatcaga aagaccgcga aggcgagcaa agcccaacgc ctcttcgccg
     9421 cgcgcccgcg ccgctccgac acgtcttcga gcagcagcag gttgccctcc gtggcgaggt
     9481 gttcgacctg tgcgcgcttg ccctgcacga gcagcacgtc tccgaagcga aggctgacca
     9541 tgctcagttt cgacaagagc gcgacgccgt gacggttgat ggcgagcgcg gtgaggccgt
     9601 agcgttcgcg aaacttcgtg cccttcaagg tgcgcccgac gagattcgag ccgcgcaaga
     9661 ccatcgcttc gaacagttcg agattgcccg cttcgaggtc tgtgtcgctc aatttgaagt
     9721 cggccttgat ctcgatgccc gcttcggtct tgacgcgcag gatgtcttcg atgcgcccct
     9781 gcacgaggag caggtcgccc gcgagaatct gctcgtctgc gcgcggcgcg atgcgcccct
     9841 gcttgccgcg caggatgccg acgacggcga ggtcgagcga ctggctgagg ttcgcttcgg
     9901 cgagcgtctg cccgacgagc ggcgacgtgt cgagcacgac gacctccgac atgtattcgc
     9961 ggatatgata ggcgtcggcc agagagtccg cgctgccgcg cgccgggagc aggcgcaggc
    10021 cgacgaggag catgtagagc atgccgacga cgacgatggc gatgccgacc ggcgtgagtt
    10081 cgaacatcgt gaagggcgcg aggttgtagc gcgtcattgc gccgctcacg gcgagattgg
    10141 tggaagtgcc gatgagcgtg caggtcccgc cgaggatcgc gccgaaggcg agcggcatga
    10201 gaagtttcga cggctgcact ttggcgcgtt ccgcgagtcc gagcacgacg ggcacgaaca
    10261 tggcggtcgt cgtcgtgttc ttcatgacgg aggcgcaggc cgccgccgtg aacatgatga
    10321 gcaccgtcag gcgaaactcg ctcccgcccg acacgcgatg cagacgccgc ccgacgagat
    10381 cgaccacgcc cgtcttgacg aggccgccgg ttaagatgaa gaggccggag atcgtgatga
    10441 tgatgtcgtt gccgaaaccg gcgaagcctt ccgcaggcgt gagcacgccc gttaagacga
    10501 ggccgatgac gacgaggatc gtcaccacgt caatcgggat gcgctcggtg ccgaagagca
    10561 cgagcgcgac gaaaaggagt atgagaaccg tggcgatgga gagagtcata aacgcttctg
    10621 ctatactggc cgctcaaaaa aggttaagca accggcgatg gcggcacaca gccgacccgg
    10681 cggttgctat cggataaatt aagggggaag ttcgtcgtga gcaagcatcg cattctcgtc
    10741 atagaagatc aagaagacct cgcggagttg tacgagtcgt cgctggagaa ggccggctac
    10801 gaggtaacga acgcctacac gggcgaagag ggcgtcgcgg aattcgaggc caacggcgcg
    10861 gacgccgtgt tgctcgacat gactctgccg gaaatgcacg gcacgaaggt gctggaagag
    10921 attcgcaatt tgtctccgag cgtgcccgtc gtggtggtg
//
LOCUS       DASWNS010000621        17915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_178082, whole genome shotgun sequence.
ACCESSION   DASWNS010000621 DASWNS010000000
VERSION     DASWNS010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 17915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17915
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178082"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VGB76_21470"
     CDS             <1..325
                     /locus_tag="VGB76_21470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101510.1"
                     /translation="LLEFPADVTGAPQRGSRALQARRTRADLLSTHVQTKASGTQLRR
                     GVNELLKQSEVVTQSDAPTVEGFEDLEDFDSYDPTHLFWEQIKQLDFALTSSKGSLIK
                     TQMSL"
     gene            complement(441..1076)
                     /locus_tag="VGB76_21475"
     CDS             complement(441..1076)
                     /locus_tag="VGB76_21475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101511.1"
                     /translation="MAEPKTKPERRVAGKSKFRLPDRPPRVVEINPLREILQEAKAQE
                     DALATPSPQTHATPAKRIAGVAQSPKTSVPDTTPAQSIAGVSVPAAAGLSSPADKDTK
                     NSFGDFSRRWAPILRSGQMSVCRVLFEMTHEVGQMECFTSMPKLATAAGLKERQCYNV
                     VGQLELLGFIERPEIFNTPTKKGTIFRLFLSPQPPDAGSNRRYHIGGKEED"
     gene            complement(1076..1888)
                     /locus_tag="VGB76_21480"
     CDS             complement(1076..1888)
                     /locus_tag="VGB76_21480"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY0101512.1"
                     /translation="MKTIAIANQKGGVGKSTTAACVATELAMRGYETLLIDGDPQANV
                     TSHFLAPDQINLSIADILIESEQRDKLPLEAAIVTTEMEHLDLVPSKLRFARFEKEPA
                     IAINRLRSKLVPLSSTYDFAVIDTPPTLGQILTTPLLASTHMLVPVAASPLAQDGLDD
                     LMSTFEEVRSTNPELEMLGMVCTLFDGRTSVSAESFRSLKERYGPKVFETIIHRNVKL
                     EESPAFHQPVQLYAPGSRGALLYAELTDEILKRLGARKNKGRLKLAVEESRA"
     gene            complement(1893..2081)
                     /locus_tag="VGB76_21485"
     CDS             complement(1893..2081)
                     /locus_tag="VGB76_21485"
                     /inference="COORDINATES: protein motif:HMM:NF027067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0101513.1"
                     /translation="MTGTDIKNLRKALGYTQAKLAEEIGITANTIARYERDELKPSPP
                     VLKLLKLLEILVIERKAA"
     gene            2647..3777
                     /locus_tag="VGB76_21490"
     CDS             2647..3777
                     /locus_tag="VGB76_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101514.1"
                     /translation="MAVAPLPAPRFIRKTQLGANEYRKNFHQIDREIIAALTSTEADQ
                     VTPLMSKIYLCLVNAPAKYWEREGVLRIEAEIREGKLVKAWAVLCDLTGVASATASKA
                     MQWMHQQGIIGYFSGKNGVGLRIFLNRAASSIGARQAQGSKKILSLFPASFVEKHTSS
                     REPAFNDSYAVREVLDTDINPGAPKNGANRALVDRVPSVPARHSPTVQLPDAADYTVM
                     LETPGLFPVDEIVSRLRQALEPAMQTAARNAAAREHDHTREWLENRGLPKAARVAQHE
                     AYNVLRKYGVISKSARTSHVPADIGRSDYIPPEPHPLKDVEIVDMAGACVAMLETRGQ
                     PIELTLSEMSIEAGGFLLPEDACRVRDKAIALLSGSDSGEGH"
     gene            3794..4612
                     /locus_tag="VGB76_21495"
     CDS             3794..4612
                     /locus_tag="VGB76_21495"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0101515.1"
                     /translation="MSISSNARRFVVVPDGEEAGSSQPRVSTVAELSVCSSCFGTGME
                     VRAGKGARRCHCRTEDTRTKLAEAARIPRRYEECSLGSYQPTPDNGSQLAAFNHAYSL
                     AREYPTVERGLLFMGTCGVGKTHLSVAILRELMEKKSVSCMFYEFGALLKEIQNSYNP
                     ISQTSELKVLAPVVEAEVLVLDELGATKPTDWVRDTMMQIINTRYNHKKLTIFTTNYT
                     DTRRTPAEETLEDRIGTRLRSRLYEMCKTVMIEGEDFRRRFNSELSGVREKNSL"
     gene            4994..5740
                     /locus_tag="VGB76_21500"
     CDS             4994..5740
                     /locus_tag="VGB76_21500"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase II family protein"
                     /protein_id="HEY0101516.1"
                     /translation="MSSSNWLTLQKPDRQAKQRLFCFPYAGGAAFIYRDWSKNLPPSI
                     EVCPVQLPGRGSRMREPFFRRIEPLVEAAAIALLPYLDRPFAFFGHSMGALISFELAR
                     LLRREHKLHVSKLFLSGRGAPHLPDREPTTYNLPEPEFIAELQRLKGTPQEVLAHPEL
                     MDLMSPILRADFEICQTYEYVPEPPLECPITAFGGLQDEEVSHRQLDAWREHTNSLFS
                     LRMFPGDHFFLHASAPVLHRMIAQELWDVT"
     gene            5975..6220
                     /locus_tag="VGB76_21505"
     CDS             5975..6220
                     /locus_tag="VGB76_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101517.1"
                     /translation="MDNTTSQRRNKHLILNQAKLKKAQKVLGAKTETEAIERALDSVI
                     DEAERNRRAWAAHDRFLRSAVRDNLQIQDAFGRLGAD"
     gene            6223..6663
                     /locus_tag="VGB76_21510"
     CDS             6223..6663
                     /locus_tag="VGB76_21510"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0101518.1"
                     /translation="MPGILFDTSVYISALRRGNASILSTRRAARKGEPHDRPLWLSVV
                     VLEELLVGARDGRMKKLLAHLERDFERAGRLLVPVQSDWTASGQVLALIGEKYGYEEV
                     GRARITNDALIAMSASRNGITVQTLNEGDYRKIAEVRPFEWERV"
     gene            7109..7333
                     /locus_tag="VGB76_21515"
     CDS             7109..7333
                     /locus_tag="VGB76_21515"
                     /inference="COORDINATES: protein motif:HMM:NF015578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HEY0101519.1"
                     /translation="MSRDEREDNTIYKVVVNHEEQYSIWPADRENALGWNDAGKSGTK
                     DECLAYIKEVWTDMRPLSLRKKMEESSQSQ"
     gene            7356..8111
                     /locus_tag="VGB76_21520"
     CDS             7356..8111
                     /locus_tag="VGB76_21520"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0101520.1"
                     /translation="MTAPDTHAASRWFTIPPEERGKIRLFCFPYAGGSSATFRSWGKA
                     LAPAVSVYPAHLPGRERRLSEKPFTRALSLVEAAAGAILPYLDRPFAFFGHSMGAMIS
                     FELARYLRRHNHPQPTHLFISGSRAPQLGFNGAVTYNLPDAEFAEELRRLNGTPREVL
                     EHPELMGLMTPVLRADFEVCQTYLYTPEPALDCPVTAFGGLLDQGVSREMIEAWEEQT
                     TARFRLWMLPGDHFFVNSAPQQILNILSRELQR"
     gene            complement(8148..14405)
                     /locus_tag="VGB76_21525"
     CDS             complement(8148..14405)
                     /locus_tag="VGB76_21525"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0101521.1"
                     /translation="MQSEVIEGFMLSPQQRSLWSEQQERQDYRALGAVIIEGDLRKDL
                     LKSAVQAIIDRHEILRTTFQQRPGMTFPLQVVGEEGTQPSWDESNLEDLGLREQEEMV
                     AQIFSREGAHRFTASEGPLVRAALLRLDARRHILSLNISPLCADAATLDNIFAQVAAA
                     YDSLAHGGDLTAEHIQYADFALWQQELTEGEDVEAGLAYWRGLEFEPLLAQPVPFERP
                     LADANAAVSFDSVAVELEPRTVAGLKTAALQYDLPIGSFLLACWQTLIQRLTGQQEIV
                     VGLHSDGRKYEDLRGACGHYAKQLPLVIHFKERSRFTDILAQLKRVSVEAEEWQEYFN
                     LEAVAAGSPQSPFLPLGFEFDEQPSPFSADGLNWTNFKRDVRTSRHKLKLRCTTTADG
                     GLRAALDFDPRYVTRESAARLAAHFSALASDTAAQPTSPIAQLELLDEHARRKLLSEF
                     NQTSAQFGAARCFHEIFAEQAAQTPERIALICEDRQLTYAELERRSNQLARYLRGRGV
                     GRETIVAIFGEREVELIVALLATLKAGAALVAFDPKYPKERLAGLVEDARPKVVLTQA
                     RLLEQLPAAAETVLLDADWPQIALESAEAVESVATPDNLAYVIYTSGSTGKPKGVTVE
                     HRQLFNYLRAAQERLDLPPQAAYATVSTLAADLGHTMIFPALASGGTLHVVSHERASD
                     PFELAEYFTRHKIDCLKIVPSHLQALQSYAHPEQLLPAERLVLGGETTRWELIEQLRA
                     LRPGCRIFNHYGPTETTVGVIACEVREGDGVEVAATLPLGRPLANTQIYLLDARLAPV
                     PVGVTGDLYVGGAGLARGYLHHPGLTAEKFIPDPFAVAPGARLYRTGDRARYLPDGRI
                     EFLGRADHQVKYHGFRVELNEIRSELNRHPQVSDSVVVIGKGKGGGGEMLVAYYVSRQ
                     EIPAAQLREFLSANILEETLPNAFVHLQKLPLTLNGKLDYQALPTVEQSRQKSAPTIV
                     SPRTPAEQILASIWTDLLGVEQISIHDNFFELGGHSLLATQVVSRLREAFRVELPMRD
                     LFEWPTVAGLAARVEEARRAGHSVAVPPIEPTGRDQALPLSFAQQRLWFLNQLEPDNP
                     AYNMFGALQLNGRLDAAALEQSINRIVARHEPVRTTFGVVDGQPVQIIAESLHLPLPL
                     TDLSTLDADARQAEVSRLSDEESKRTFDLAAGPLLRLSLLRLSEEEHVLLVSMHHIIS
                     DAWSIAIFTRELAAFYEAAATGRTCELPALRVQYADFAHWQREWLQGENLERELAFWK
                     KQLEGAPPVLELPTDRPRPEVQSFRGARLRQLLPTELLDGLKTLSRSEGSTVFMTLLA
                     AFQVLLQRYTGQQDIVVGTDIAGRNRQELENLIGFFVNHPVLRTSLEGNPSFRELLKR
                     VREVTLDAFAHQDLPFDKLVSALRPDRNLSRMPIFQVLLVLRNTPIEPLQLSGLKLSL
                     LETENLTSKFDVALFMEETAGGLAAVWNYSTDLFDSDTIERMARHFEGLLYSAVAQPD
                     VKLDEFEISGLDTPAARVRPQTASAPVAPRLAPASGGEPQNELEETLVAIWSQLLGAE
                     KIGIHDNFFELGGDSIISIQIIARASQQGIRLTPRQFFLHQTIAELAAVAGTTAAVKA
                     EQGLVVGTVPLTPVQQTFFARNLPEPHSFTQAVLIETGLPLDPSLLEQTVRQLLLHHD
                     ALRLRYERDADGWRQTNSGEPSEVPFAQLDFSALPEGELDDAIEAAIEAAQSSIDLSS
                     GALLKVTRIEQGAGRPDLVLLTVHHLAIDVVSWRILIEDLQSVYLQLSEGRRPRLAEK
                     TTSFKRWAERVVEHVGAGGFGEEAGYWLDELRGSGRALPLDFADGQNTLSSARVVTST
                     LDADETRALLQEVPQAYHTQVNEVLLTALARAFAEWSGNGSLLVEMEGHGREEVFDDV
                     DLSRTVGWFTSLYPVLLETEKKAGAGDELKRFKEKLRSLPNRGLGFGLLRSFGTGDEA
                     ARLRERPRPEVGFNYLGQFDLENAEASSFGRVRSSRGASGLMPGARTHLLEVDAKVVA
                     GRLQVDWTYSENLHRRETVEALAGQFERALRALIEHCRSPEAGGFTPSDFPDAELSQA
                     QLDNLFAKINA"
     gene            complement(14487..16241)
                     /locus_tag="VGB76_21530"
     CDS             complement(14487..16241)
                     /locus_tag="VGB76_21530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051994 - P-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhpK family radical SAM P-methyltransferase"
                     /protein_id="HEY0101522.1"
                     /translation="MNIPQTLDCIVIGYNEVPFQHYEKFLRNFGENSEAYRDLKFSFV
                     DVGGEKMDYVGLLNHVIRSSDPEGRSELAREGVKSGDIPNLAAAYLSNFLRQHGCETR
                     TINLFQYEKEKLAGYLAQDPVCVAITTTFYVVNMPINEMVEFIREHNPKTKIVVGGPL
                     IANHARNYTGDNFRIAVDDLGADIYVIEGQGEATLQRVVECLKRGGSLEEVPNLAYLE
                     DGQLKRTRTVPEDNSLDENYIDWRNFPEEKLGVTLQTRTARSCAFKCSFCNYPTRAGA
                     LSLANLDTLEREFDSMRELGDVSNVVIIDDTFNVPLPRFKEICRLLIRKNYGFNWFSY
                     FRCSNSDEEAFELMAESGCKGVFLGIESGSPTILKNMNKAATPEKYARGIEALKRHNI
                     LTFGSFIAGFPGETEQTINETIDFIKQTKPDYYRTQMWYCEPGTPIYQQKEKYEINGD
                     GFVWSHATMDSIGAMGHIERMFLNIDESLWLPQWSFDFWIIPYLIGKGISPERFKTFM
                     GMAHKLLALEIASVSGAEKETLQQQYLARGTEAATRWLQPAGASRGGLSLGMSKRLRS
                     GGRVATPVVEPAIPGTER"
     gene            complement(16284..>17915)
                     /locus_tag="VGB76_21535"
     CDS             complement(16284..>17915)
                     /locus_tag="VGB76_21535"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0101523.1"
                     /translation="LLAAFKTLLHRYTGAEDVLVGSPIANRTRAETDDIIGFFLNALP
                     LRTDLSGDPTFSELLARVRQTTLGAYAHQDVPFEKLVEELRPERQMTHTPFFRTWLVL
                     QNAPMPQMQLPGISLSMMHVDDGTSKFELVLSMYETEEGLHAMWVYNSDLFDAATVAR
                     MAGHFETLLSSVSQRPDEQLSALEIYTAAEKQRRSQEKSKREESNLKKLKSLRRSGIN
                     LKQVSLTKTEYLAEGEMLPLVVRPAGDVNLAEWVRGNRDYIEGELQRHGALLFRGFSV
                     NSPERFEELALAVCPQLFTEYGDLPRQDTGSSRVYHSTPYPEASMILYHNESSHIHQW
                     PLKIMFYCDTAARSGGETPIVDGRRVYRDLDPGLRRRFEEKRLMYVRNYKEGIDVSWQ
                     SFFGTDNRAEVESYCRRAGIEFEWTSDDGLRTRKVAPAVVRHPRNGEPAFFNQMQAHH
                     ISCLAPDLRHALLSLYAEEDLPRNVYYGDGTPIEDSVVDEVREVYRRAAVVFPWVAGD
                     ILLVDNMLTAHARNPFVGPRKILVAMGDMVSHAGY"
BASE COUNT         3487 a         5180 c         5613 g         3635 t
ORIGIN      
        1 cctactcgaa tttcctgctg acgtgaccgg agcacctcag agaggctctc gggcgctcca
       61 agcccgtcgc acgcgcgctg acttgctctc gactcatgtg caaactaaag cctccggcac
      121 acagttgcgc cggggcgtga atgaattgtt gaagcagtct gaggttgtga cacaaagtga
      181 tgcgccaact gtcgaaggct ttgaggattt ggaagacttc gactcttacg acccgacgca
      241 tctgttctgg gaacagatta agcaactgga tttcgccttg acgagttcga aggggtcatt
      301 gataaagact caaatgtctt tatagcagta aagaagaact gtaaggactc gaaggcagag
      361 agagtaatat ttagaattta aagctgctca ctacccctgc aatttgcgtt gcagcccctg
      421 taataaagat tgcaggggtg tcagtcctct tccttgcctc cgatgtgata tcgacggtta
      481 gacccggcat cggggggctg tggcgagaga aagaggcgaa agatagttcc cttcttagtg
      541 ggggtattga agatttcagg tcgctcgatg aagcctaaca actcaagctg cccaaccacg
      601 ttataacact ggcgttcttt taggccagca gcggtagcca atttaggcat cgacgtgaaa
      661 cattccattt gtcccacttc gtgggtcatc tcaaagagca cgcgacacac actcatttgt
      721 ccggaacgta agatcggtgc ccagcgtctg gaaaaatctc caaaggaatt tttggtgtct
      781 ttgtctgccg gactcgacag tcccgcagcg gcagggactg acacccctgc aatgctctgt
      841 gcaggggtag tgtccgggac agatgttttc ggagattgcg ccacccctgc aatcctcttt
      901 gcaggggtgg catgggtttg aggtgacggc gtcgcgagcg catcttcctg agctttggcc
      961 tcttggagaa tctctcggag ggggtttatc tcgacaacgc gcggcggtcg gtcaggaagc
     1021 cggaatttgc ttttaccagc tacccttctc tccggttttg ttttcggttc agccatcagg
     1081 cgcgcgactc ctcaaccgcc agttttagtc tccccttgtt cttgcgggca ccgagccgtt
     1141 ttagtatctc gtctgtcagt tcagcataga gcaacgcacc gcgcgaaccg ggagcgtaaa
     1201 gctgtacggg ttgatgaaag gccggtgact cttccagctt gacgttacga tgaatgatcg
     1261 tctcgaagac cttcgggccg tatcgctcct tgaggctgcg aaaagactct gcggagactg
     1321 aggtgcgccc gtcgaataga gtacagacca tgccgagcat ctcaagttcc ggattggtac
     1381 tccgtacttc ctcaaacgtg ctcatcagat catccagacc gtcttgagcg agcggcgagg
     1441 cagccaccgg tacgagcatg tgcgtcgagg cgagaagcgg ggtagtcaaa atttgaccca
     1501 gcgtaggcgg agtatcaatg accgcaaaat cataggttga agagagcggg acgagctttg
     1561 atcgcagacg gttaatagcg atagcaggct ctttctcaaa tcgcgcgaaa cgtagtttag
     1621 aaggcaccag atcgagatgt tccatctcgg tcgtcacaat ggctgcttcc aacgggagtt
     1681 tatcgcgctg ttcagactca atcagtatgt cggcgatgga aagattgatc tggtcgggcg
     1741 cgagaaaatg agaggtgaca tttgcctgag gatcgccatc tataagcagt gtttcatagc
     1801 cgcgcatagc gagttctgtg gccacacagg ccgcggtggt gcttttgccc acgccgccct
     1861 tttgattagc gatggcaatc gtcttcatcc ctttacgccg ccttcctctc aatcacaagt
     1921 atttcgagca gcttgagcag tttcagcacc ggcggcgagg gtttcagttc atcgcgctcg
     1981 tagcgcgcaa tcgtgttggc tgtgatgccg atctcctcgg ccagtttcgc ctgcgtgtac
     2041 cctaatgctt ttcgtagatt ttttatatct gtgcctgtca tataaggatg cctctcagat
     2101 gagaacgccg catattacta tacgaagcgt atatacgtca ggaatatttc tgaggtcaat
     2161 acccctgtaa tcttcgtcgc accccctgta ataaacattg caggggtatg cattttaagt
     2221 tcgacgccgc atcatctact tcaacaaacg aatttcgcct cgaaaaccca acagacgaca
     2281 cccctgcaat cttcagtgca ggggtcgcac tatagattgc aggggtatac acccctgtaa
     2341 tctacgttgc aggggatgca acgattctta caggggatgc aatgtagatt gcagggggtg
     2401 caatgaatag tgcaggggga tttacaggct agattaaatc gtgtaggaat gaacgccgga
     2461 ttgagagacg cgatttaggc atcgcaaaat aagcattggc ctgaagctcc agagtttcgc
     2521 agaccgtaaa gcttcaggcc aatttcacaa ctcgcttcga tagcgagtcg ggattcgtgt
     2581 accttctact cgcattgtac gcccttccgg ctatcgcttt caagcttatg aagaaaggtt
     2641 ttcgtcatgg ccgtcgcgcc gcttccagca ccacgcttca tacgcaagac gcaactcggg
     2701 gcgaacgagt accgcaagaa tttccatcag atcgaccgcg agatcatcgc ggcgctcact
     2761 tcgacagaag cagaccaagt gacaccgctc atgagcaaga tatacctgtg tctggtcaac
     2821 gcgccggcca agtattggga gcgtgaaggc gtgctgcgca tcgaagcaga aatcagggaa
     2881 gggaagttag tgaaagcatg ggctgtgctt tgcgatttga cgggcgtggc ttcggcgacg
     2941 gcgagcaagg ccatgcagtg gatgcaccag cagggaatta tcggttactt ttccgggaag
     3001 aatggagtcg gactgagaat cttcctcaat cgggcggctt catctatcgg cgcgagacag
     3061 gctcaaggca gtaaaaaaat tttgagcctt tttcctgctt cattcgttga gaagcatact
     3121 tcatcacgtg aaccagcctt taatgacagc tacgctgtta gagaagtttt agacacagat
     3181 attaatcccg gcgcgccaaa aaacggcgcg aaccgagcgc tggtagatag agtaccttcc
     3241 gtaccggcac ggcacagccc aaccgttcaa cttccggacg ccgcagacta caccgtgatg
     3301 ttggagacgc cgggtctgtt tccagttgac gagatcgtct cccgattaag gcaggcattg
     3361 gaacctgcga tgcagacggc agcacggaat gcggcggcgc gcgagcatga ccacacgcgc
     3421 gaatggctgg agaaccgagg gctgccgaaa gctgcacgag tggctcagca cgaagcttac
     3481 aacgttctca ggaagtatgg cgtgatcagc aagtccgcgc gaacttcgca cgtgcctgct
     3541 gacatcggac gcagcgacta tatccctccc gaaccgcacc cactcaaaga tgtagagatc
     3601 gtcgatatgg caggagcctg cgtcgcaatg ctcgaaacca gagggcagcc tatcgaactg
     3661 actctctcgg agatgagcat agaggccgga ggatttttgc tgccggagga tgcttgccgg
     3721 gtgcgcgata aggcaattgc tttgctctcc ggcagcgata gcggggaagg gcattgagag
     3781 gagggattcg cctgtgtcaa tcagttcgaa tgccagaaga tttgttgtcg ttccagacgg
     3841 ggaagaagcc ggttcttcgc agccgcgagt ttcaactgtc gcggaactgt ccgtctgctc
     3901 ctcctgcttc ggcaccggaa tggaggtcag ggcaggcaag ggcgcacgac gttgccactg
     3961 tcgcacagag gatacccgaa cgaagttagc ggaggcagcg cgcatcccac gccgttacga
     4021 agagtgctcg ctcggcagct accaacccac gcccgacaac ggctcccaac tcgcggcatt
     4081 caaccacgct tacagtctcg cgcgcgaata tccgacggtc gagcgcggcc tgctttttat
     4141 ggggacgtgc ggcgtcggaa agacgcatct ctcggtcgct atcctgcgtg agttgatgga
     4201 gaagaaaagc gtgtcctgta tgttctacga attcggagcg ctcttgaagg agattcagaa
     4261 ttcttacaat ccgatctctc agacatcaga gttgaaagtg ctcgcgcctg tcgtcgaagc
     4321 ggaagttttg gtgctggacg aactgggcgc gaccaagccg accgactggg tgcgcgacac
     4381 gatgatgcag attatcaaca cgcgttacaa tcataagaag ttgaccatct ttacgaccaa
     4441 ttacacggac acacgccgca cgccagcgga ggagacgctg gaggatcgca tcgggacacg
     4501 gctgcgcagc cgtctctacg aaatgtgcaa aactgttatg attgagggcg aagacttcag
     4561 gcgaaggttc aactctgagc tctccggcgt tcgtgaaaaa aactctctct gacgatagac
     4621 acgacttcgc gtttcgacat cgccgaatcg aacgaggtgc ggcttgaaca caggctccaa
     4681 agagcagttg taactcgttt ccctgtgcgc ttacgtcgaa ctctcattgt gaacacggcg
     4741 tccgctatcg cgtggactaa ctcctgagaa actgcacgcg cgcagttcgc tgatcaaagg
     4801 ctttccataa tctgtaggca cataaatttg catggtatat ttaataaaga aaagcgtggc
     4861 tctcaaaaac tgtccgaaag tttgagcgag gaataaagag aataaccgcg tgatgatact
     4921 tcttgaaacg gtcagctttc agtgccacat tctgttcgaa cgtgatgtcg tcaagggagt
     4981 gggttaaata aacgtgtctt catccaactg gcttacgctt cagaaaccag accggcaagc
     5041 gaagcagagg ttgttctgtt ttccctacgc gggcggggct gccttcatct atcgtgattg
     5101 gtcgaaaaac cttccgccga gcatagaagt ctgtccggtg caactcccgg gacgcggcag
     5161 cagaatgcgt gaaccgttct tcaggcgaat agaaccgttg gtcgaagcag ccgccatcgc
     5221 gctcctgcct tatctcgacc gcccgttcgc tttcttcggt cacagcatgg gcgctctcat
     5281 cagtttcgaa ctagcccgcc tgctccgccg ggagcataag ctccacgtct cgaagttgtt
     5341 cctctcagga cgcggcgcgc cgcaccttcc agaccgggaa ccgacgacct acaacctgcc
     5401 cgaaccggag ttcatagcag aactgcaacg tctaaaggga actcctcaag aggtgctggc
     5461 acatccggaa ttaatggact tgatgagtcc aatcctgcgc gcggatttcg aaatttgtca
     5521 gacctatgag tacgtgccgg agccgccgct ggaatgtccc atcacggcct tcggcggctt
     5581 gcaggacgaa gaggtttcgc accggcaact tgacgcgtgg cgcgaacaca ccaactcttt
     5641 attctccctg cgcatgtttc cgggcgatca cttcttcctg cacgcgtccg cgcccgtgct
     5701 gcaccgcatg attgcacagg aactatggga cgtgacgtga accgaacacg cgacacggag
     5761 aaactcaccc gcctttgtcc ttcgcaggcc gacaaagcgc tcccgctaat ccggcagttc
     5821 gtcaacagtc acgcgtcctt aaatggaaga acgaacccct agtaaaacac aaatttcgcc
     5881 cggagtagtg acggcttgcg gcggtctagc ccggaacgta acttttcttg ttggtgtaca
     5941 tgccggatgt tattatgtac atgaggtaag tgacatggac aacaccacga gccagcgccg
     6001 gaacaaacat ctgatcctca atcaggccaa actcaaaaag gcgcagaagg ttttgggggc
     6061 gaagaccgaa accgaggcca tcgagcgcgc actcgactcc gtgattgacg aggccgagcg
     6121 caaccgtcgc gcctgggctg cgcacgaccg cttcctgaga agcgctgtgc gagataacct
     6181 tcaaatccaa gatgctttcg gtcgcctggg ggctgactag ccttgcccgg catcctcttc
     6241 gacacctccg tctacatctc tgccttgcgg cgtggaaacg cctcgatcct ctcaacacgg
     6301 cgcgccgcgc ggaagggtga gccgcatgac cgtccactgt ggctgagcgt cgtggtcctg
     6361 gaggaactcc tggtcggcgc gcgggacggg aggatgaaaa agctgctggc gcacctggag
     6421 agggattttg agcgtgcggg aaggctgctc gtcccggtcc agtcggactg gacggcgagc
     6481 gggcaggtgt tggctctcat cggcgagaaa tacggctacg aagaagtagg gcgggcgcgt
     6541 atcaccaacg acgcgctgat cgctatgagc gcgtcacgca atggaatcac cgtccagacg
     6601 ctaaacgagg gagactaccg caagatcgcc gaggtgcgcc ccttcgagtg ggagcgcgtg
     6661 tagccttcac ggggaaaaag atgcggtctg acgccggact cttgaaattg tacggccaga
     6721 actttgaagc cgtgcccgcc cggcgtgcat cgcgtcgaaa tgaaatacgc ggcaccgtcg
     6781 gcgcgcaccg gcgcgtggat ttcgctcgcc gccttgctgc tgttaacgtc tctcgcgctc
     6841 cccggcaagc ggtctgcaaa accctgcgcg cgatcttata gccgggtcaa cgcctgccct
     6901 cagacgcgtc taataacgga tgctgccgtt ctcccaaaag tcgaaataga tcctctttga
     6961 agtgacccgc cttcgacgca atcccttctt gcaacctgcc ttccttcaag gtacactgcg
     7021 gtttcacttc agcggtttca cttcaccggt tttacttcac cggaaatctt cattaaacat
     7081 ctatcccaac ccaaaaacag gagcctctat gagccgcgac gaacgagaag acaacacgat
     7141 ctacaaggtg gtcgtcaacc acgaagaaca gtattccatc tggcctgctg accgtgagaa
     7201 tgcgctcggc tggaacgacg cgggcaagag cggcacgaag gatgaatgcc tcgcctacat
     7261 caaagaggtg tggacggaca tgcgccccct cagcctgaga aagaagatgg aagagtcttc
     7321 gcagtcgcag tagccgccat cacctgagcc gcaccatgac cgcgccagac actcacgccg
     7381 cgtctcgctg gtttactatt ccgccggagg agcggggcaa gattcgtctg ttctgttttc
     7441 cttacgcggg cgggagcagc gcgaccttcc gcagttgggg gaaggcgctc gcgcctgccg
     7501 tctcggtcta tccggcgcac ctgcccggac gcgagaggag attgtctgag aagcctttca
     7561 cgcgcgccct gtcactggtg gaagccgccg ccggtgccat cctcccgtat ctcgaccgtc
     7621 ccttcgcctt cttcggccac agcatgggag ccatgatcag ttttgaactc gcgcgctacc
     7681 tgcggcgaca caaccacccg cagccgaccc atctctttat ctccggcagt cgtgccccac
     7741 aactcggatt caacggcgcg gtcacgtaca acctgccgga tgcggagttt gcggaagagt
     7801 tgcgccgact gaacgggacg ccgcgcgagg tgctcgaaca ccccgaactg atgggattga
     7861 tgacgcctgt tttgagagcg gattttgaag tttgccaaac ctatctttac acgcctgagc
     7921 ctgcgctcga ctgccccgtc acagccttcg gcgggttact tgatcagggc gtgagccgcg
     7981 agatgatcga agcctgggag gaacagacca ccgcccgctt ccggctgtgg atgctccccg
     8041 gcgaccactt ctttgtaaac tccgcgccgc agcagatcct caatattctc tcacgcgaac
     8101 ttcaacgctg atccgcctct gatagcgacg ctatgttccg tctttcgcta agcgttgatc
     8161 ttggcgaaca ggttgtcgag ttgcgcctgc gagagttccg cgtcggggaa gtctgagggc
     8221 gtgaagcctc ccgcctcggg cgagcggcaa tgttcgatca gggcgcgtag cgcacgctcg
     8281 aactgccccg cgagcgcctc cacggtttcg cgccggtgga gattttcgct gtaagtccaa
     8341 tctacttgca gccgaccggc gacgactttc gcgtccactt ccagcaggtg cgtccgcgcg
     8401 ccgggcatca gcccactcgc tccgcgcgac gatctgaccc gaccgaaaga tgaggcttcg
     8461 gcgttctcca gatcaaactg tccgaggtag ttgaagccga cctccgggcg cgggcgttcg
     8521 cgcaggcgcg cggcttcgtc tcccgtgccg aaggagcgca ggaggccgaa gccgaggccg
     8581 cgattcggca ggctccgcag cttctctttg aagcgtttca gttcgtcacc cgccccggcc
     8641 ttcttctccg tctcaagcag cacggggtag aggctcgtga accagccgac cgtgcgcgac
     8701 aggtctacgt catcgaagac ctcttcgcgc ccgtgaccct ccatctcgac caggagcgag
     8761 ccgttcccag accattctgc gaaggcacgt gcaagcgcgg tcaggagcac ttcgttgacc
     8821 tgcgtgtggt aagcctgcgg aacctcttgc aacaaggcgc gcgtctcgtc cgcgtcgagc
     8881 gtcgaggtga cgacgcgggc agaagagagc gtgttttgcc cgtcggcgaa atcgagcggc
     8941 agggcacgcc cgctcccgcg caactcgtcg agccagtagc ccgcctcctc cccgaagccg
     9001 cccgcgccga catgctcgac cacgcgttca gcccaacgct tgaacgaggt ggtcttctcc
     9061 gcgagtcgag gtcggcggcc ttcgctcaat tgtaggtaga cggattggag gtcttctatc
     9121 aggatgcgcc acgagacgac atcgatggcg aggtgatgta cggtcagaag gacgaggtct
     9181 ggcctgcccg ccccttgctc tatgcgcgtg accttgagca gcgcgcccga agagaggtca
     9241 atggaagact gcgcggcttc gatggctgcc tcaatggcgt cgtccagttc gccttcgggc
     9301 agcgccgaga agtccagttg cgcgaacggc acttcggacg gctcgccgga gttcgtctgc
     9361 cgccagccgt ccgcgtcgcg ctcgtagcgc aggcgcagcg cgtcgtgatg gagcaggagt
     9421 tggcgtaccg tctgttcgag cagcgaagga tcgagcggca gacccgtttc gatcagcacg
     9481 gcctgtgtga aggagtgcgg ctccggcagg tttcgcgcaa agaacgtctg ctgcaccggc
     9541 gttagcggga cagtgccgac aacaagaccc tgctcggctt tcacggcggc ggtcgtgccc
     9601 gcgacggcgg ccagttcggc aatggtctgg tgcaggaaga actgtctggg cgtgagccgg
     9661 atgccctgct ggctggcgcg cgcgatgatc tggatgctga tgatcgagtc gccgccgagt
     9721 tcgaagaagt tgtcgtgaat cccgatcttc tccgcgccca gaagttgcga ccagatggcg
     9781 acgagcgttt cttccaactc gttctgcggc tcgccgccgg aggcaggcgc gagacgcggc
     9841 gcgacgggcg cggacgcggt ttgcggcctg acccgcgcgg cgggcgtgtc gaggccggag
     9901 atttcaaact cgtcgagttt tacgtcgggc tgtgcgacgg cgctgtagag caacccctcg
     9961 aagtgtcgcg ccatgcgctc gatggtgtcg gagtcgaaga ggtctgtgct gtagttccac
    10021 accgccgcca gcccgcccgc agtctcttcc ataaacagcg ccacgtcgaa cttcgatgtg
    10081 aggttctctg tctccaacag gctcaacttg aggccggaga gttgcagcgg ttcaatcggg
    10141 gtgttgcgca gcaccagcag cacttggaag atcggcatcc ggctgaggtt ccggtcaggc
    10201 cgcagcgcgc tgacgagctt gtcgaagggc aagtcctgat gggcgaaggc gtcgagcgtc
    10261 acctcgcgga cgcgcttgag cagttcgcgg aaactcgggt tgccctcaag gctcgtacgc
    10321 aggacggggt ggttgacgaa gaagccgatg agattttcga gttcctgccg gtttcgtccg
    10381 gcgatgtccg tgccgacgac gatgtcctgc tggccggtgt agcgttggag gagaacctga
    10441 aaggccgcga gcagcgtcat gaagaccgtg cttccttcgc tgcggctgag agtcttcaag
    10501 ccgtcgagca actccgtcgg cagtagttgg cgcagacgtg cgccgcgaaa gctctgcact
    10561 tcggggcgcg ggcggtcggt cggcagttcg aggacgggcg gcgcgccttc gagttgcttc
    10621 ttccagaagg cgagttcgcg ttcgaggttt tcgccctgaa gccattcgcg ctgccagtgt
    10681 gcgaagtcgg cgtactgcac gcggagcgcg gggagttcgc aggttcgccc cgtagcggcg
    10741 gcttcgtaga aggccgccag ttcgcgcgtg aagatggcga tagaccacgc atcgctgatg
    10801 atgtggtgca tggagactaa gagcacgtgc tcctcttcgc tcaggcgcag gagcgacagg
    10861 cgcagcagcg gcccggcggc gaggtcgaac gttcgcttcg attcctcgtc gctcaaacgc
    10921 gaaacctctg cttggcgcgc gtctgcgtcc agtgttgaga ggtctgtgag cggcagcggg
    10981 aggtgtagcg actcggcgat gatctgcacg ggctgcccgt caacgacgcc gaacgtcgta
    11041 cgcacaggct catggcgcgc gacgatccgg ttgatgctct gttcgagcgc ggcggcgtcg
    11101 aggcgtccgt tgagttgcaa agccccgaac atattgtagg cggggttgtc gggttcgagc
    11161 tggtttaaga accagagacg ctgctgcgcg aaggagagcg gcagggcttg gtcgcgtccg
    11221 gtcggctcga tgggcggcac ggcgacggaa tgacccgccc gccgcgcctc ctcgacgcgg
    11281 gctgcgagtc cggcgaccgt cggccactcg aagagatcgc gcatcggcag ttccacccgg
    11341 aaggcttcgc gcaggcgcga cactacctgc gtcgccagca gcgagtggcc gccgagttca
    11401 aagaaattgt cgtggatgct gatctgttcc acgccgagca agtctgtcca gatggaggcg
    11461 agaatttgct cggcgggcgt gcgcggcgag acgatggtgg gcgcggactt ctgccgcgac
    11521 tgttcgaccg tggggagcgc ctgataatcg agtttcccgt tgagcgtcag cggcagcttt
    11581 tgcagatgaa cgaaggcgtt cgggagcgtc tcttcgagaa tgttggcgga gaggaattcg
    11641 cgcaactgcg ccgccggaat ctcctgccgc gagacgtaat aggcaacgag catctcgccg
    11701 ccgccgccct tgcccttgcc gatcacgacg acgctgtcgc tgacctgcgg gtggcggttc
    11761 agttccgacc tgatctcgtt gagttcgacg cggaagccgt gatacttcac ctgatggtcg
    11821 gcgcgcccga ggaactcgat ccgcccgtcg ggcaggtagc gtgcgcggtc gccggtgcgg
    11881 tagaggcgcg cgcccggcgc gacggcgaac gggtctggaa taaacttctc agccgtcagg
    11941 ccgggatggt gcaggtagcc gcgcgcgagt cccgcgccgc cgacgtagag gtcgcccgtg
    12001 acgccgacgg gcaccggggc gagccgtgcg tcgagcagat aaatttgggt gttggcgagg
    12061 gggcgtccga gcggcagcgt cgccgcgacc tcaactccat ccccttctct gacctcacag
    12121 gcgatcacac cgacggtggt ttcggtcggt ccatagtggt tgaaaatgcg gcagcccggt
    12181 cgcagcgcgc gaagctgctc gatcagttcc catcgcgtcg tctcgccgcc gagcacaagc
    12241 cgttcggcgg gcaggagttg ttcgggatgc gcgtaggact gcaaggcttg caggtgagaa
    12301 gggacgatct tgagacagtc gatcttgtga cgcgtgaaat attcggccag ctcgaagggg
    12361 tcggaggcgc gctcgtgtga gacgacgtgg agcgtgccgc cgctcgccag tgcggggaag
    12421 atcatcgtat gcccgaggtc tgcggcgagg gtcgagacgg tggcgtaggc ggcctgtggg
    12481 ggaaggtcga gccgctcctg tgcggcgcgc aggtaattga acaattgacg gtgctcaacc
    12541 gtcacgccct tcggtttgcc cgtcgagccg gaggtgtaga tgacgtaagc gagattgtcg
    12601 ggcgtggcga cgctctcgac ggcctctgcg ctctcaagcg cgatctgcgg ccagtcagca
    12661 tcgaggagca ccgtctcggc agcggcgggc aactgttcga gcagtcgcgc ctgcgtcagc
    12721 acgactttcg ggcgcgcgtc ttcgaccaag cccgccagac gctccttcgg atatttggga
    12781 tcgaaggcga ctagagcggc tccggccttg agcgtggcga gcagcgcgac gatcagttca
    12841 acctcgcgct cgccgaagat ggcaacgatt gtctcgcgcc cgacgccgcg cccgcgcagg
    12901 tagcgtgcca gttggttcga cctccgttca agctccgcgt aggtgagttg cctgtcttcg
    12961 caaatcagcg cgatgcgttc gggcgtttgc gcggcctgct ccgcgaaaat ctcgtggaaa
    13021 cagcgagccg cgccgaactg cgccgacgtt tgattgaatt cggagaggag cttgcggcgc
    13081 gcgtgctcgt cgaggagttc gagttgggcg atgggtgacg tgggctgcgc ggcggtgtcc
    13141 gaggccagcg cggagaagtg tgcggcgaga cgcgccgcgc tctcgcgcgt gacatagcgc
    13201 gggtcgaagt cgagggcggc gcgcaggcca ccgtccgccg tcgtcgtgca gcgcagtttc
    13261 agcttgtggc ggctcgtgcg gacatcccgt ttgaagttgg tccagttcaa tccgtccgca
    13321 gagaagggcg agggctgctc gtcgaactcg aagccgagcg gcaggaaggg cgactgcggc
    13381 gagcctgccg cgaccgcctc aaggttgaaa tactcctgcc actcttcggc ctcgaccgag
    13441 actcgcttga gttgcgcgag gatgtccgtg aagcgcgagc gttccttgaa atggatgacc
    13501 agcgggagtt gcttcgcgta atgcccgcac gcgccgcgca ggtcttcata ctttcgtccg
    13561 tcgctatgga ggccgacgac gatctcctgc tgccccgtca aacgctggat cagggtctgc
    13621 cagcaggcga gcaggaacga gccgattggg aggtcgtatt gcagtgccgc cgtcttcaac
    13681 ccggcgacgg tgcgcggctc aagttcgacc gcgacggaat cgaagctgac ggctgcgttc
    13741 gcgtccgcca gaggacgctc gaagggaacg ggctgcgcga ggagcggctc aaactcaagc
    13801 ccgcgccaat aggcgagtcc cgcctccaca tcttcgcctt cggtcaactc ctgttgccac
    13861 agggcgaagt ctgcgtactg aatatgttcc gccgtgaggt cgccgccgtg agcgagcgag
    13921 tcgtaagcgg cggcgacctg cgcgaagatg ttgtcgaggg ttgcggcgtc ggcacagagc
    13981 ggcgagatgt tgagcgacag gatatgccgc cgcgcgtcga gccggagcag cgcggcgcgg
    14041 acgagcggcc cctcgctcgc tgtgaagcgg tgcgcgcctt cccttgaaaa aatctgagcg
    14101 accatctctt cctgctcccg caagccgagg tcttcgaggt tgctttcgtc ccacgaaggc
    14161 tgcgtgcctt cttcgccgac gacctgtagg gggaaggtca tgccggggcg ttgttggaaa
    14221 gtcgtgcgga ggatctcgtg ccggtcgatg atggcctgga ccgcgctctt gagcaggtct
    14281 ttgcgcaggt cgccttcgat gatgaccgcg ccgagcgcac ggtaatcctg tctctcctgc
    14341 tgctccgacc acaggcttct ctgctggggc gaaagcataa agccttcgat aacttcgctc
    14401 tgcatcactc tctccttaac ttcgattcaa aagcccgggg cgcactgagc acagtcgcgg
    14461 gggaaggtct taaccggacg aaggggttaa cgttcggtgc cggggatcgc cggttcaacc
    14521 acgggtgtcg ccacacgccc gccgctcctg agtcgcttcg acatgccgag cgagaggccg
    14581 ccgcgcgacg cgcccgcggg ttgcagccag cgcgtcgcgg cctctgtccc gcgcgcgaga
    14641 tactgctgct ggagcgtctc tttctccgcg ccggagacgg acgcgatttc gagcgcgagg
    14701 agcttgtgcg ccatccccat gaaggtcttg aaacgttcgg gtgagatgcc cttgccgatc
    14761 aggtagggga tgatccagaa gtcgaaggac cactgcggca gccagagaga ctcgtcgatg
    14821 ttgaggaaca ttcgctcgat gtgccccatc gcgccgatgc tgtccatcgt ggcgtggctc
    14881 cagacgaagc cgtcgccgtt gatctcatac ttctccttct gctggtagat gggcgttccc
    14941 ggctcgcagt accacatctg cgtgcggtag tagtcgggct tcgtctgctt gatgaagtcg
    15001 atggtttcgt tgatcgtctg ctcggtttcg ccggggaagc ccgcgatgaa ggagccgaag
    15061 gtcaggatgt tatgacgctt gagcgcttcg atgccgcgcg cgtatttctc gggcgtggcc
    15121 gctttgttca tgttcttgag gatggtggga gaacccgact cgatgccgag gaagactccc
    15181 ttgcagccgc tctcggccat caattcaaac gcctcttcgt ccgagttgct acagcgaaaa
    15241 taggagaacc agttgaaccc gtagttcttc ctgatgagca ggcggcagat ctccttgaag
    15301 cgcggcaacg gcacgttgaa ggtatcgtcg atgatgacga cgttcgacac gtcgcccaac
    15361 tcgcgcattg agtcgaactc gcgctcaagc gtgtcgaggt tggcgagcga gagcgcgccc
    15421 gcacgcgtcg ggtagttgca gaacgagcac ttgaaggcgc aactgcgagc ggtgcgcgtc
    15481 tggagggtga cgccgagttt ctcttcgggg aagttgcgcc agtcgatgta gttctcgtcg
    15541 agcgagttgt cctccggcac ggtgcgcgtg cgcttcaact gcccgtcctc caggtaggcg
    15601 aggttgggaa cttcttcgag gctgccgccg cgcttgaggc actcgacgac gcgttggagc
    15661 gtcgcttcgc cctgcccctc gatgacgtag atgtccgcac cgaggtcatc gacggcgatg
    15721 cggaagttgt cgcccgtgta gttgcgcgcg tggttggcga tgagcggacc accgacgacg
    15781 atcttcgtct tggggttgtg ctcgcggatg aactcgacca tctcgttgat cggcatgttg
    15841 acgacataga aggtcgtcgt gatggcgacg cagacggggt cttgggcgag atacccggcc
    15901 agtttctctt tctcgtactg gaagagattg atggtgcgcg tttcgcagcc gtgctggcgc
    15961 aggaagttcg agaggtaggc ggcggcgagg ttcgggatgt cgcccgactt cacgccctcg
    16021 cgcgccaact cagaccgccc ctccgggtcg gaactcctga tgacgtggtt gaggaggccg
    16081 acgtagtcca tcttctcgcc gcccacgtcc acgaagctga acttcaggtc gcggtaagct
    16141 tcggagttct cgccgaagtt gcggaggaat ttttcatagt gctggaaggg gacttcgttg
    16201 tagccgatga cgatgcagtc gagcgtctgt gggatgttca ttcttattct ccggatggtt
    16261 tgaggcggaa gcagaccgcg cgcctagtag cccgcgtgag aaaccatgtc tcccattgcg
    16321 accagaattt tgcgcggccc gacgaacggg ttgcgcgcgt gcgccgtgag catgttgtcc
    16381 acgagcagga tgtctccggc cacccacggg aagacgaccg ccgcgcggcg atagacttcg
    16441 cgcacctcgt ccacgaccga gtcctcgatg ggcgtgccgt cgccatagta gacgttgcgc
    16501 gggaggtctt cttcggcgta gagcgacaag agcgcgtggc gcaggtcggg cgcgaggcag
    16561 gagatgtggt gcgcctgcat ctggttgaag aaggccggtt cgccgttgcg cgggtggcgc
    16621 acgacggcgg gcgcaacttt gcgcgtgcgc agcccgtcgt cgctcgtcca ctcgaactcg
    16681 ataccggcgc ggcggcaata gctctctacc tccgcgcggt tatcggtgcc gaagaaagat
    16741 tgccagctca cgtcgatccc ttccttgtag ttgcggacgt acatcagtcg cttctcctcg
    16801 aagcggcggc ggagtcccgg gtcgaggtcg cggtagacgc ggcgcccgtc aacgatgggc
    16861 gtctcgccgc cgctgcgcgc tgccgtgtcg cagtagaaca tgatcttgag cggccactgg
    16921 tgaatgtggg aactctcgtt gtgatagagg atcatgctcg cttcggggta gggcgtcgag
    16981 tggtagacgc ggctactgcc ggtgtcctgc cgtggcaggt cgccgtattc ggtgaagagt
    17041 tgcgggcaga cggcgagcgc cagttcctcg aagcgttcgg gcgagttgac gctgaaaccg
    17101 cgaaagagca gcgcgccgtg gcgttgcaac tcgccctcga tgtagtcgcg gttgccgcgc
    17161 acccactcgg cgaggttcac gtcgcccgcc ggtcgcacga cgagcggcaa catctccccc
    17221 tcggcgagat attcggtctt cgtcaggctc acctgcttga gattgatgcc ggagcggcgc
    17281 aggctcttga gcttcttcag gttcgactcc tcgcgcttgc tcttctcctg cgagcggcgt
    17341 tgcttctcgg ctgcggtgta gatttcgagc gcgctcagtt gctcgtcggg tcgctgcgag
    17401 acacttgaga gcaacgtctc gaagtggccg gccatgcgcg ccaccgtcgc ggcatcgaag
    17461 aggtctgagt tgtaaaccca catcgcgtgc agcccttcct ccgtctcgta catcgagagc
    17521 acgagttcaa acttcgaggt gccgtcgtcc acgtgcatca tgctcagaga gatgccgggc
    17581 agttgcatct gcggcatcgg tgcgttctgg aggactagcc aggtgcggaa gaaaggcgtg
    17641 tgcgtcatct gccgttcggg tcgcaactct tcaaccaact tctcgaacgg cacgtcctga
    17701 tgcgcgtatg cgccgagcgt cgtctggcgc acgcgggcga gcaactcgct gaaggtcggg
    17761 tcgccggaga ggtctgtgcg cagcggcagc gcgttgagga agaagccgat aatgtcgtcc
    17821 gtctcggcgc gcgtgcggtt ggcgataggc gagccgacga gcacgtcttc tgcgcccgtg
    17881 tagcggtgca ggagcgtctt gaaggccgcg agcag
//
LOCUS       DASWNS010000622         7947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_49981, whole genome shotgun sequence.
ACCESSION   DASWNS010000622 DASWNS010000000
VERSION     DASWNS010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49981"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..652
                     /locus_tag="VGB76_21540"
     CDS             377..652
                     /locus_tag="VGB76_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101524.1"
                     /translation="MKAGRPDSFAACDGARKMLVIHTGLGFERGYNGSANVIVAARYG
                     QVQTGDGSDAETGLTILCVERVTPIGSEIGGRIRYVMEEVSRRIGFY"
     gene            779..1243
                     /locus_tag="VGB76_21545"
     CDS             779..1243
                     /locus_tag="VGB76_21545"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0101525.1"
                     /translation="MSAIRFFERDVSEAELAQVNAGFDEHTIEHGNPIEIQERYSVVV
                     MDGETFVGSASGLAYKGNGVYNNWFYLSDLFIQKSYRGQGIGAAALRRLEVRVAALGI
                     RNMWTWTAGYEAPGFYKKQGYEVFCELENWYFTGHSKVGLRKTLGTEPARPS"
     gene            1346..1639
                     /locus_tag="VGB76_21550"
     CDS             1346..1639
                     /locus_tag="VGB76_21550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101526.1"
                     /translation="MTTPAVVTGDDTAKGITRMAFNNKTKIGFTYDGSIDAFGAVYFY
                     VPSDRLGIAITSNGQNYPSGEIFWTVLRIVYGAREESGHYEDRSSRGFHAPLM"
     gene            complement(1752..3032)
                     /locus_tag="VGB76_21555"
     CDS             complement(1752..3032)
                     /locus_tag="VGB76_21555"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0101527.1"
                     /translation="MKRVLWLALFVLSLAGVEAIAQPPQPQQVTAIKAGKLVVPETGA
                     TLTNQTILVEGSRIKAVGAGLQIPAGATIVDLSNSTVLPGLFDCHTHMAFLVNRRGRE
                     RGSLYFYDLTHTNAERAIHAVMNSRDMLSAGFTTIRDIGNDGNYVSTDLRRSIEQGLI
                     PGPTVVNSGRIIAPYGGQNQLQPERRHLGEPEYFYADTRDEMRKAIRENAHYGARVIK
                     IVVDDQPYIYSVEDIKYMIAEAASAGLKLAAHCVTEQGARNAAEAGVASIEHGFEMSD
                     EALALAKKNNVVLVSTDFSEKAWLAYDIPPQVAKRFYERSVDRLRRAHKIGVTLAFGS
                     DLVFYAPDETRGTWSISLIDVFVAAGVPPKEILRALIPNAARLLGVERVRGAISAGQF
                     ADIIATPDNPLDNISTLKQVNFVMKNGKVFKAAK"
     gene            3431..5113
                     /locus_tag="VGB76_21560"
     CDS             3431..5113
                     /locus_tag="VGB76_21560"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0101528.1"
                     /translation="MRKIAALLLFASISLTPLNAQTSAARPTPPQERIDAEMYWKIRR
                     EGMERSQAMRTLHVLTDVYGPRLTGSPNFKGAADWTVKQMESWGFRNAHLEPWDFGHP
                     GWLNERLSAHIVSPVKDALVCEALAWTPGTNGAVTGQAFQLVLPERPTRESFTAFLDG
                     IRNQVRGRIVLVGRHQFVPVTFNPQNKRREDQEARNQLDPNAPPTPSPFAAPSPSPAA
                     SPQASPTPAPPLTQQQIGEQLDQFLVASGVLVRINDAGREHGQIRAFNNRTFDVTKAV
                     PTVVMRNEDYGRISRILADGTPVELEFNIVNRSYPEGRTAYNVIGEIPGTDKADEVVM
                     LGGHLDSWHAATGATDNAIGCTIMLEAARILHTLGVKPRRTIRVALWSGEEQGLLGSK
                     AYVKEHFGTFENPKPEYFKFNGYFNIDSGTGRVRGANVFGPPETATILRQAFAPFEDL
                     GVMGAIATTSRRTGGTDSTSFNEAGLPGIGLSQDPIEYGTHTWHTNLDTYERIVENDV
                     KIAAIVVAAGVYHLAMREEMLPRFNRERMPAPPPTPTPSPSPSPTPAPSPRG"
     gene            complement(5271..5813)
                     /locus_tag="VGB76_21565"
     CDS             complement(5271..5813)
                     /locus_tag="VGB76_21565"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY0101529.1"
                     /translation="MPLPIAHALVGASLAAAIHTRPDTFRYRVALISGAILANAPDLD
                     FFLVFTLHSKAWHRGFTHSFVFAGFVCLLFVLLFLLTSGKRPLRDALAYGLAFASHGI
                     LDFVTTKAGGGVELFWPFSAERLLLGWAGLSELPSRLPPLTILKWLAFEFAIFAPVLL
                     AVLFWRRTVQRRADSPTRAG"
     gene            complement(5863..6102)
                     /locus_tag="VGB76_21570"
     CDS             complement(5863..6102)
                     /locus_tag="VGB76_21570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101530.1"
                     /translation="MNTNIRIIKRSQREAQSKTATATTANSNRARSNENPRHATRDIS
                     GHVATWIKEFQQKHTPDPRRAFAKLFIDTAASAAD"
     gene            complement(6306..6692)
                     /locus_tag="VGB76_21575"
     CDS             complement(6306..6692)
                     /locus_tag="VGB76_21575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101531.1"
                     /translation="MTSSDGKTETPFTLSFTGEGAATQLELMNATDEPLRCVEILTVF
                     LKDEETSAAPSRAHIRFEAVKYIQPREKAVMPHKTLIDGKPAAPADDQLQRLKAVTGT
                     TKPYVLDISWEDAEGKSRFQRIPVGH"
     gene            complement(6731..6952)
                     /gene="infA"
                     /locus_tag="VGB76_21580"
     CDS             complement(6731..6952)
                     /gene="infA"
                     /locus_tag="VGB76_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY0101532.1"
                     /translation="MAKEDLVPAEGVVIDKLPNAFFKVKLDGGEHLVLAQIGGKMRKN
                     HIRILAGDRVSVELSPYDLTRGRITYRYK"
     gene            complement(7039..7398)
                     /locus_tag="VGB76_21585"
     CDS             complement(7039..7398)
                     /locus_tag="VGB76_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY0101533.1"
                     /translation="MSMKLYVGNLAFQTSSDDLQELFAQAGTVESASVVEDRETGRSR
                     GFGFVEMSSAEEGAKAIEQFNGKEVNGRSLNVNEAKPREDRGGRGGGGGGGGRGGYGG
                     GGGGRGGYGGGGGGNRY"
BASE COUNT         1584 a         2317 c         2383 g         1663 t
ORIGIN      
        1 cgcgctctac tgcattgtta ggccgcatcc tgcggtcgag catgcgttat actttttctc
       61 gttgtagaga gggaatatga atctgcggcg cattgggatc aggctcggca tcgcactcct
      121 tatagtgctg ctctgcctgc tcgccggttc catctttatc tcccgtgaaa gcgaggccga
      181 gcccgccccc tgccccgcct gttcggcagt ttacgcctct gccgcttcgg aggtacccac
      241 cgtaaccccc tgcgacctcg ttcaagaccc gcagagatac aacaaccgtg tagtccgggt
      301 tcgggcgatc tggagacatg actcgggcac agtctctctg tcagacccgg cagccgggtg
      361 cgagggtggc tcgtcgatga aggccggacg accagattca tttgccgcat gcgacggcgc
      421 gcgtaaaatg cttgtcattc acacagggtt aggtttcgag cgcgggtaca acggcagcgc
      481 taatgtgatc gtggccgccc gctacggcca agtccaaact ggtgacggct ctgacgcaga
      541 gacgggactg acgatcctgt gtgttgagcg ggtgacgccc atcggctcgg agataggcgg
      601 gaggatacgt tacgtgatgg aagaagtctc aaggcggatc gggttttatt aggccgtgcg
      661 tcacacatct atcgcttgat gggacagcag gtattgactg ccggacattc cgaaaatact
      721 ttgcttattg atctgacagg agccatcgcc tgcgggtatt cttgccggag gacacgatat
      781 gagtgctata cgttttttcg agcgcgacgt gagtgaggcg gaacttgctc aggtaaacgc
      841 ggggttcgat gaacatacaa tcgagcatgg caatcctatc gagattcagg agcggtacag
      901 cgtcgtggtg atggacggcg aaacctttgt cggctctgcg agtggtcttg cctacaaggg
      961 gaacggcgta tacaacaact ggttttactt gtctgactta tttatccaga aatcttatcg
     1021 cgggcaagga atcggagcgg cagctctgag gcgacttgaa gttcgggtag cggcgcttgg
     1081 catcaggaat atgtggacgt ggactgccgg ctatgaagcc cccggatttt acaaaaagca
     1141 gggctacgag gtcttttgcg aactggaaaa ctggtacttc accggacata gcaaagtcgg
     1201 cctgcgtaaa actttaggaa ccgaaccagc gcgcccatcc tgaaccacgc tacgacaagt
     1261 cgttggacgt aagggcgaaa cacgagatgg gcgatgaact gatagtttcg ataaaaaatt
     1321 gataagcgaa gagtctctgc gaataatgac gacgcctgcc gtcgtcacgg gcgacgacac
     1381 cgccaaaggt atcacccgga tggctttcaa caacaaaacc aagatcggct tcacctatga
     1441 cggttcgatt gatgcgttcg gcgcggtcta tttctacgtg ccttcggata gactcggaat
     1501 tgccattacg agcaacgggc agaactatcc ttccggcgaa attttctgga ctgtgttgag
     1561 aatagtctac ggcgcacgcg aggagagcgg ccactatgaa gaccggagca gccggggttt
     1621 tcatgcgccg ctcatgtgag aattttgccg ttgacgtatg tgttcatcct gcttccctcc
     1681 tccgttctga tccgttcaga agtgatcgct tcaagcgtgc gcagtgcgcg ctcgaagcga
     1741 tcacgcgccg tctacttcgc agccttgaag actttgccgt tcttcatcac gaagttgacc
     1801 tgcttcaagg tcgagatgtt atcgagcggg ttgtccggcg tggcgatgat gtctgcgaac
     1861 tgtcccgcgc tgatcgcgcc gcgcacgcgt tcgacgccga gcaggcgcgc ggcgttcggg
     1921 atgagcgcgc gcagaatctc cttcggcggc acaccggcag cgacgaaaac atcaatgagt
     1981 gaaatcgacc aggtgccgcg cgtctcgtcg ggcgcgtaga agacgaggtc gctgccgaag
     2041 gccagcgtga ctccgatctt gtgcgcgcgt cgcagacgat ccacgctgcg ttcgtagaag
     2101 cgttttgcga cctgcggcgg aatgtcgtaa gccagccacg ccttttcgct gaagtctgtg
     2161 ctgacgagca cgacgttgtt cttcttcgcc agcgcgagtg cctcgtccga catctcgaag
     2221 ccatgttcaa tcgaggcgac gcccgcctcc gcagcattgc gcgctccctg ctccgtgacg
     2281 cagtgcgccg ccagtttcag ccccgcgctc gccgcctccg cgatcatgta cttgatgtct
     2341 tcgaccgaat agatgtaggg ctggtcgtca acgacgatct tgatcacgcg tgcgccgtag
     2401 tgcgcattct cgcggatggc cttgcgcatc tcgtctctgg tgtcggcgta aaagtattcc
     2461 ggctcgccga ggtgtcggcg ttcgggttgg agttggttct ggccgccgta gggggcgatg
     2521 atgcgcccgg agttgacgac ggtggggccg gggatgagtc cctgctcaat cgagcggcgc
     2581 aggtcagtcg agacgtagtt gccgtcgttg ccgatgtcgc ggatggtggt gaagcctgcg
     2641 ctcagcatgt cgcgggagtt catgacggcg tggatggcgc gctcggcgtt ggtgtgcgtc
     2701 aggtcgtaga agtagagcga cccgcgctcg cgcccgcgcc ggttgaccag aaaggccatg
     2761 tgcgtgtggc agtcgaacag accgggcaac accgtggagt tggagagatc aacgatggtc
     2821 gcgcccgcag gtatctgtag tcccgcgccg accgccttga tgcgcgagcc ttcgactaaa
     2881 atggtctgat tcgtgagcgt cgcgcccgtt tccggcacca ccagtttgcc cgccttgatg
     2941 gccgtcacct gctgcggttg tggcggctgc gcgattgctt caactccggc gagtgagagg
     3001 acgaacaggg caagccagag aactcttttc atttatttcc tttcgattaa cgctttgcgc
     3061 tgagattggg tttgtaggct ggcagatagc tgcctccgct cgatacatag ccttacgatc
     3121 tagagcgcgg gaggtttcgg cattttcata gacgacctga gaggttccgg ttgaccggaa
     3181 gaatcgagcc ttaagaatcg agccttgagg agcggcaact ttcatttaaa gtcgcgccgc
     3241 cggagagtag attgaaaccc cggaggcgtc aacttagtaa gtctcaggtc gcaagtctca
     3301 agtcggcagg gctttcggat ttgagactta gaatccggaa cctgcgactt gcgacctgag
     3361 acttgtcaga cggatgtgtt ataagaagtc gtccgaccaa aaattcaaca cacattggag
     3421 ttatcagatc gtgagaaaga tcgccgcgct tctcctgttc gcgtcaatat cgctgacccc
     3481 gctcaacgct caaacttccg ccgcccgtcc gacgccgccg caggaacgca tcgacgcaga
     3541 gatgtactgg aagattcgac gtgagggcat ggagcgttcg caggctatgc gaaccttgca
     3601 cgtcctgacg gacgtttacg ggccgcgtct gaccggctcg cccaacttca aaggcgcagc
     3661 cgactggact gtcaagcaga tggaatcatg gggctttcgt aacgcgcacc tcgaaccgtg
     3721 ggacttcggc catcccggct ggttgaacga acgcctctcc gcgcacatcg tctccccggt
     3781 caaggatgcg ctcgtctgcg aggcgctcgc ctggacgccc ggcacgaacg gcgcggtcac
     3841 gggtcaagcc ttccaactcg tgctgcccga acgcccgacg agggagagct tcacggcgtt
     3901 tctcgacggc atcagaaatc aagtgagagg caggatcgtg ctcgtcggca gacaccagtt
     3961 cgtgcccgtc actttcaacc cgcagaacaa gcggcgcgaa gaccaagaag cgcgtaatca
     4021 gctcgacccg aacgccccgc cgacgccttc gcctttcgcc gcgccctcgc cttcgcccgc
     4081 cgcctcgcca caggcgtcgc ccacgcccgc gccgccgctg acgcagcagc aaatcggcga
     4141 gcaactcgat caatttctcg tcgccagcgg cgtgctcgtc cgcatcaatg acgccggacg
     4201 cgagcacggg cagattcgcg ccttcaataa tcgcaccttc gacgtgacga aagccgtgcc
     4261 cacggtcgtc atgcgcaacg aagactacgg gcgcatcagt cgcattctgg ccgacggaac
     4321 gcccgtggag ttggagttca atatcgtcaa tcgcagctac ccggaggggc gcaccgctta
     4381 caacgtcatc ggcgagattc cgggcacgga caaggcggac gaagtggtga tgctcggcgg
     4441 ccacctcgat tcatggcacg ccgcgacggg cgcgaccgac aacgccatcg gctgcaccat
     4501 catgctcgaa gccgcgcgca tcctacacac gctcggcgtc aagcctcgac gcaccatccg
     4561 cgtcgccctc tggagcggcg aagagcaggg cttgctcggc tcgaaggctt acgtcaaaga
     4621 gcatttcggc acgtttgaaa atccgaagcc ggagtatttc aagtttaacg gttatttcaa
     4681 catcgactcg gggacggggc gcgtgcgtgg cgcaaacgtg tttggaccgc cggagacggc
     4741 gaccattctc cggcaggctt tcgcgccctt tgaagattta ggggtaatgg gagcgattgc
     4801 caccacgagc cgccgcacgg gcggaaccga tagcacctct ttcaacgaag caggattgcc
     4861 gggcatcggc ctcagccaag accccatcga atacggcacg cacacctggc acacgaacct
     4921 cgacacctac gaacgcatcg tcgagaacga cgtgaagatc gcggccatcg tcgtcgccgc
     4981 aggcgtctat catctcgcca tgcgcgaaga gatgctgccg cgcttcaaca gagagcggat
     5041 gcccgcgccg ccgccaactc cgacgccgtc tccgtcgccg agtcccacgc ccgcaccgtc
     5101 gccgcgcggc taaagcgtgc tcgcaaaaac gcgcttcaac tctttgcgcc ttaagtcatg
     5161 accctgcgct tccggcgaga actctttcac gccggaagcg caaaagttaa gacttaaggc
     5221 gcaaagtttt gagtcgcgag gtgaagtttc gggactatct ttaactatct ttaaccggcg
     5281 cgtgtgggcg agtctgcgcg tcgttggact gtgcgccgcc agaacagcac ggcgagcagc
     5341 acgggcgcga agatggcgaa ttcgaacgcg agccatttca ggatagtgag cggcgggagg
     5401 cgcgacggca actccgacag ccccgcccaa ccgagcagca ggcgctccgc agagaacggc
     5461 cagaacaact ccacgccgcc gcccgctttg gtcgtcacga aatccagtat gccgtgcgaa
     5521 gcgaaggcca gcccgtaggc gagcgcgtct ctcaggggtc gcttgcccga agtcaggagg
     5581 aagagcagga cgaagagcag gcagacgaag ccggcgaaga cgaacgagtg tgtgaaccct
     5641 cgatgccatg ctttggaatg gagtgtgaag acgaggaaga agtccaggtc gggcgcgttg
     5701 gcgaggatcg cgccggagat gagagctaca cggtagcgga acgtgtcagg ccgtgtgtga
     5761 atcgcggcgg cgaggcttgc gcccaccagc gcatgtgcaa tcggcaacgg catcgtgccg
     5821 ccactctaag ttcagcctgc gcgggctgtc aaacgccgcc tgttaatccg ccgcagaggc
     5881 cgccgtgtcg ataaacagtt tggcgaaggc gcggcgcggg tcgggtgtgt gtttttgctg
     5941 aaattccttg atccaggtgg cgacgtggcc gctgatgtcg cgcgtggcgt ggcgcggatt
     6001 ttcgttactg cgcgcgcggt tgctgttggc cgtcgtggcg gtcgccgtct tgctttgcgc
     6061 ctctctctga gagcgtttga tgattctgat attggtgttc atggctgtat ctccgtcgtt
     6121 cagtcggcca gtctgtcctc ttaaaaacgt cttgaatggg tttccaatta acgtccccat
     6181 ctactaacgc gagatttaga ctgaaaacgg atcatccgaa attgtagccg ggcgggaggt
     6241 ggccggaact agccggagcc gcgctggcga cgtgactcca tgagaggtgt gtagggcggg
     6301 ctgcttcagt gcccgacagg tatcctttga aagcgtgatt tgccttcggc gtcttcccag
     6361 gaaatatcca gcacgtaggg cttggtcgtg cccgtcacgg ctttgagccg ttgcagttgg
     6421 tcgtctgccg gggctgccgg tttgccgtca atcaaagtct tgtgcggcat cacggccttt
     6481 tcgcgcggtt ggatatactt gaccgcctcg aacctgatgt gcgcgcgcga gggcgcagcc
     6541 gaagtctctt cgtctttgag aaagaccgtg agaatctcca cgcaccggag cggttcgtcc
     6601 gtggcgttca tcaactccaa ctgcgtcgcc gcgccttcgc cggtgaagga taaggtgaaa
     6661 ggagtctctg ttttaccgtc gctgctggtc ataatcttta cttttccggg cgagcgcgct
     6721 cgcctgcgtc ttatttgtac ctgtaagtga tccggcctct ggtcagatca tacggcgaca
     6781 attcgacgct cacgcgatca ccggcgagga tgcgtatgtg attcttgcgc atcttgccgc
     6841 cgatttgagc caacacaagg tgttcgccgc catcaagttt aactttgaaa aaagcattcg
     6901 gtaatttatc gatcaccacg ccttccgccg gaaccaggtc ttctttagcc aatacgctct
     6961 cctttgtttg ccgctgattt ggacgtatcc tgtgcggcgg gtgcgctatc tggcgcaccc
     7021 gccgacaggc tttgacaatt agtagcgatt gccgccaccg ccgccgccgt agccgccgcg
     7081 accaccaccg ccgccgccgt aaccaccacg accgccgcca ccgccaccgc cgccgcgtcc
     7141 gccgcgatct tcgcggggtt tcgcttcgtt gacgttcagg ctacgaccgt tgacttcctt
     7201 gccgttgaac tgctcgatgg ctttcgcgcc ttcttccgcg ctcgacatct cgacgaaacc
     7261 gaaaccgcgt gagcgtcccg tttcgcgatc ttcaaccact gaagctgatt cgaccgtacc
     7321 tgcctgcgcg aagagttcct gcaaatcgtc gctcgaagtt tggaaagcga gattgccgac
     7381 gtaaagtttc atagacataa aatagtatcc ctctcctctg taggagtgtt tggagaagcg
     7441 tcgaagcgaa ggtggcttag gagtaacggt aggtaggcga aagctctcag ttcgtgtgct
     7501 cggtaaggag cggcttctaa tgttgaaatc agccttctcc cggattaccc gaaacgcccc
     7561 gccgcttttg cggctctgtg cgtatcaccg ttaatgcaca agagaaggat tagctgacag
     7621 ccagtatgca ctataaattc ctgaaacgca agacgataac tcagtccggc ggcgcggcgc
     7681 aaagcctctc ccacgctccg ccccccttcc ccaaatcgca tcaagtgcta gaatggcgcg
     7741 tttaactcaa accttgtcgg gtgtaatttt ttgacctcat ccgtgaattt caagtcagaa
     7801 ggacgaaaga tcgggcgcat gctgagcctc gatgcgatgc gcggcgtggc ggcggtcgtg
     7861 gtcgtcgtct atcacgcgct cggggttgcg ccgcagacgg cctttgtcgg ctggcaatgg
     7921 tggctgccgc tcgtcgcctc ctacatc
//
LOCUS       DASWNS010000623        13609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_386169, whole genome shotgun sequence.
ACCESSION   DASWNS010000623 DASWNS010000000
VERSION     DASWNS010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13609
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386169"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VGB76_21590"
     CDS             <1..755
                     /locus_tag="VGB76_21590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101534.1"
                     /translation="GLVPLTFAPGVAGAAAMPNFSYVRKLGSGLPADTDDNANDFMLV
                     STGGEAFGATASVLGAPGPESSTSPAQRNAQIKASLVDPQVSSSGVPNRARLGVPVTN
                     GQYGTFVIRRKFTNKTGASITRLRFRIVDITTYPRSNNATADLRLINSPDMEITLTPV
                     NGEAATVTVKGTSLEQSSAQPQGGGINSTMMVALPGGALAPNNSINVQFVMGVEQNGS
                     FRFIVNVEAATAAAPPPAGAAGKRVGTKQGSR"
     gene            complement(868..2088)
                     /locus_tag="VGB76_21595"
     CDS             complement(868..2088)
                     /locus_tag="VGB76_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101535.1"
                     /translation="MTTPLRPLTILCIASYEKGQEFMRECKRQGCRVLLLTSHSIRDA
                     DWPRESLDDIFYMPDQNHKWVLEDVIHSVSYIARTEEIDRIVPLDDFDLETAAALREH
                     LRVPGMGDTTTRYFRDKLAMRMQALEAGIAVPEFAHVLNYERLRDYMRRVPAPWLLKP
                     RFEASATGIKKIKAPDELWPALDTLGNRQCYHVLERYVPGDIYHVDSIVYNSEVLFAA
                     VNRYGRPPLDVAHGGGIFTTRTELRGTPDEVALQEINRRVLSAMRLPHGVSHTEFIKG
                     REDGKFYFLETSARVGGANIVELVEAATGINLWAEWAKVEIAGGKPGAYELPPRRENY
                     AGLLVSLARQQSPDTSSYDDPEIVWRMNKQSHVGLIVMSHDPARIEYLLNDYSARFYR
                     DFHAVHPPTERPTS"
     gene            complement(2104..2475)
                     /locus_tag="VGB76_21600"
     CDS             complement(2104..2475)
                     /locus_tag="VGB76_21600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101536.1"
                     /translation="MTNDRAAAPLPPPQQMLLHLIGEMLRRYGDDALQSANVEAAARA
                     WLEAGFTDAEEVEEWLGARCFEPRHAQTLEAAGITPEQAALRTRAGRAGYEETVACKF
                     SRGDLTLEEARRIITSDFWNS"
     gene            2641..3285
                     /locus_tag="VGB76_21605"
     CDS             2641..3285
                     /locus_tag="VGB76_21605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101537.1"
                     /translation="MTFTTVGVGGFASNAGKTTLMCELLRALEPGWEAIKITRGHYRS
                     CGKDPQACCVSHLLSEQPVIRSGRAETYAAGKDTGRYWDAGAANVHWLIVTDKQVERG
                     IKSALERVRAKGVLIEGTSFVQFVEVDLMLMAARAAGEGKIKPSARRALAKTDALYLF
                     DEDAGATETRARFAASPFGAAGENLTGSIPVYTREDLPRLVERVREIQRSGVAA"
     gene            3318..3500
                     /locus_tag="VGB76_21610"
     CDS             3318..3500
                     /locus_tag="VGB76_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101538.1"
                     /translation="MSDKDRRKKDGERKDGDRAEMHEAVAERKAGQEPAQLPRDKSNH
                     LIGDDETAEQLKDDAS"
     gene            3517..4005
                     /locus_tag="VGB76_21615"
     CDS             3517..4005
                     /locus_tag="VGB76_21615"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY0101539.1"
                     /translation="MAEGRGYIYQLNCSDGGVPKLPVREAQLTPTGLSGDRQAHPRFH
                     GGPERALCLYALEQIHALQAEGHPIFPGSVGENVTIAGLDWPSLAPGVRLALGDEVVI
                     ELSSYTTPCRSIRRSFSGGKFGRISQKTHAGESRLYARVLRTGKLAVGQVVRVLDAGA
                     AG"
     gene            4026..4625
                     /locus_tag="VGB76_21620"
     CDS             4026..4625
                     /locus_tag="VGB76_21620"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEY0101540.1"
                     /translation="MKSNRSALILVDIQNDFCPGGSLAVKDGDRIVPVVNELQKHFDL
                     IVATKDWHPPGHASFISLWPPHCVQETAGAEFVEGLDTSRIARVFLKGTDPEIDSYSG
                     FFDNEHKRATGLGDYLQARGVNEVTIAGLATDYCVKFTALDAVMLGFETSVVVDACRG
                     VEVNEGDTARAVAEMSAAGVKIRESRQLLEAQAAKEQEA"
     gene            4721..5014
                     /locus_tag="VGB76_21625"
     CDS             4721..5014
                     /locus_tag="VGB76_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101541.1"
                     /translation="MQRRRTFTLLLMLNIILLSNGCTSDTVRPSSNGNNANTGGVGNN
                     STGSREGIGPKDTGIGGTSEGNANSAVKSNSNVEPSGINKNAGRGAPANSNQP"
     gene            complement(5149..6870)
                     /gene="rpoD"
                     /locus_tag="VGB76_21630"
     CDS             complement(5149..6870)
                     /gene="rpoD"
                     /locus_tag="VGB76_21630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY0101542.1"
                     /translation="MIEKDRADDGLRPVGFGGDKYLSFDGVGEEQHDDAISTSKLEDV
                     LQKLGGASLTEADSDELLIEEVAAVAADETDDPLDAEDELDLSAGVDDKSSDPVRTYL
                     RQMGTVPLLTREQEVSIAKRIERGQNRTHKSLARSPIALKELLQIGEDIDAGMLSIRD
                     VVAFADQADVADLEDKTEEYQRWTLEGIEKINTIFQQGLRDWEKLRAEQQVTRGKRSK
                     KMLRLRRKVAGARLEIAQEMKQLTLKDATRQRLIDAIAAVHKDIRSAEREIEKYTDKL
                     HAKRIKPEQEKEFKKHISAAKKRLKEIEAEHQVSILEIKRSHQSIARSVAETIEAKSE
                     LTEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMRGVEKFDWRRGYKFSTYATWWIR
                     QAITRAIADQARTIRIPVHMIETINKLVRTSRALVQELGREPTSEEIAQKVDMPVSKV
                     RSVFKLAQQPISLETPIGEEEDSHLGDFIEDKGRVNPADSVVAANLREITDEVLATLS
                     PREEKVIKMRYGLGPVGEEHTLEEVGKDFSVTRERIRQIEAKALRKLRHPSRSRLLKA
                     FIEGVQH"
     gene            complement(7295..7519)
                     /locus_tag="VGB76_21635"
     CDS             complement(7295..7519)
                     /locus_tag="VGB76_21635"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0101543.1"
                     /translation="MDCEESLPLLSEYHAGSLDDTQLDGIRRHLDDCPPCCDVYHDVE
                     TIVVSASSLRGEEDISFPDEQAVWQRIQFS"
     gene            complement(7568..8164)
                     /locus_tag="VGB76_21640"
     CDS             complement(7568..8164)
                     /locus_tag="VGB76_21640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0101544.1"
                     /translation="MSAAETNKEATETTPKVSDAELAERSAAGDREAFEQLYHRHFRR
                     VYALCLRMTTNPSTAEDLTQEVFIHLFNKIGSFRGESAFTTWLHRMTVNHVLMYFRKS
                     STRSELLTHEGETPVQIVRGTENADKMPVVDRIALEKAISELPPGYRSVFILHDVEGY
                     EHEEIGRMLGTSTGTSKSQLHKARLKLRDLIRRQAANN"
     gene            8348..10381
                     /locus_tag="VGB76_21645"
     CDS             8348..10381
                     /locus_tag="VGB76_21645"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEY0101545.1"
                     /translation="MKTPNTKRALCLLYAALCLAWAVIPLCTHAEAAASNWRRVRSEN
                     FVVVSNAGEAEARAGAARLEELRAIFARLQGTDSFDVTLPVTVILFQNDGDYEFFKPM
                     YRGDLRRDVAGYFLFSPDINYITLSMETARGREPHSVLFHEYVHALVRNNYAGAPLWL
                     NEGLAQYYSEFELSKDRRRVNLGRQLTHRLDPLARGEFLPLETLLSVDASSPYYQQRG
                     KHTVFYAQSWAFVHYLLSDASGARQTQLTRYLELVGAGATVEESFREAFKVGFGQMES
                     ELRAYVRAAHYRERSESFAEPVPRSLDAKLESRALTEAEVQATLGDLLLHAGREEDAE
                     EYLTRALKLDAALASGHSSLGLLRVKQERLSEAKEHLERAIRVEPEHYLARFYYADVL
                     RREGLETEKTVTGYAATTRLIRAELKKVIELAPDFLEAYALLGLVDLERSPQVEETFA
                     LLRRAAALAPKRQEFKLLLAQLHLRLEAFGSARQILEPLAHDRRSARTRAEAQALLER
                     VATNEAQAALQRRAQSIEPDNATTSAGATSGAPDVKPPGDATTTPLSDAAPVGVEALP
                     CDMPQPGPQFKPLRFDGQQACGQLVSIECEDAGGVTLSVETPERTLKLRGPALNRIRF
                     VSYTADVRGRVECGLRTHANPVLVTYRPLKDSRAAAIDGEVVAVEFVPPDWVH"
     gene            complement(10525..11460)
                     /locus_tag="VGB76_21650"
     CDS             complement(10525..11460)
                     /locus_tag="VGB76_21650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEY0101546.1"
                     /translation="MILIVNLNLAVDHIVEVDELRAGGVQRSRGERRQAGGKGVNVAR
                     VLKTLGEPSLLLGFLGGRAGRLIKAGLREEEIASRFTRVENESRTCLIINDRSRREQT
                     VINAPGASISDANWRSFEASYKRSLPAAGYVVITGSLPPDLPDQTYARLIEAAHARGK
                     RVLLDCAGASLRHALEARPFLVKINGAEASELLGLPVVTEFDGAARAARKLRESGAES
                     ASITLGAQGALFDFGAAQYRFSAPQVEAVNSVGSGDATLAGLAAGLCRGWTVQESGAL
                     AIAAGAANALHGAGACSLEEILELQPRVRCEVENF"
     gene            complement(11492..11983)
                     /locus_tag="VGB76_21655"
     CDS             complement(11492..11983)
                     /locus_tag="VGB76_21655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0101547.1"
                     /translation="MRSALPPKDKYLIGVISDTHGLMRPEALKALAGADLILHAGDIG
                     RPEVLDALRNLAPVVAVRGNNDQEAWAKAFAETEIVEIGEVRLYLLHALQELTLDPSA
                     SGFDIVVCGHSHRPSSERRTDGVLYLNPGSAGARRFKLPVSVALLTVRGKSCRARIVE
                     LKV"
     gene            12155..13114
                     /locus_tag="VGB76_21660"
     CDS             12155..13114
                     /locus_tag="VGB76_21660"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY0101548.1"
                     /translation="MVNYIDLLLVAIVLLSAFYGWYRGFILGLLDLLRWIGSLLLGLR
                     FYQPLARWLAPHFDWSEAWDRPVAFIFTAFVAGLAIQLLGNALIRRLPKDVHERRLNR
                     IFGIVPGAASGLITAAITAALLMAAPLPDGLQARARESTLANRLAASTEQLETALAPV
                     FNDAIAQTLNRLTVKPESNESVELPFKVADTKPRPELEAEMLELVNRERVAAGLSTLA
                     ADDELREVARRHSSDMFARGYFSHVTPEGRDPFARIREAGYTFRTAGENLALAPTLSL
                     AHTGLMNSPGHRANILRPQFGRVGIGIMDGGRRGLMVTQNFRN"
     gene            complement(13222..>13609)
                     /locus_tag="VGB76_21665"
     CDS             complement(13222..>13609)
                     /locus_tag="VGB76_21665"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0101549.1"
                     /translation="DLTDEHRAKLAGFDIPITNQRIEKLEGDAETKQIRHVRFEDGDS
                     LECDALFFNLGTEPASTLHEMLDCRMDEECGLVWVDADQQTSVPGVYAAGDLTPKSQL
                     AVVAAAEGAMAAIHIHKSLVPESQRV"
BASE COUNT         2529 a         4235 c         4220 g         2625 t
ORIGIN      
        1 cggggcttgt gccgttgacg ttcgcgcccg gcgtcgcagg cgcggcggcg atgccgaatt
       61 tcagctacgt gcgcaaactc ggcagcggcc tgcccgccga caccgacgat aacgcgaacg
      121 acttcatgct cgtctcgacg ggcggcgaag cgttcggcgc aaccgcctcc gtgctcggcg
      181 cgccgggtcc ggaaagctct acgagtccgg cgcagcgcaa cgcacagatc aaagcctcgc
      241 tcgtcgatcc gcaagtctct tcttcgggcg tgccgaaccg tgcgcgcctc ggcgttccgg
      301 tcacaaacgg gcagtacggc actttcgtca tccggcgcaa gttcacgaac aagacgggcg
      361 cgagcatcac gcgcctgcgc ttccgcatcg tggacatcac gacctacccg cgctcgaaca
      421 acgcgacggc ggatctgcgc ctgatcaact cgcccgacat ggagatcacg ctcacgcccg
      481 ttaacggcga agcggcgacc gtgacggtga aaggcacttc actcgaacag tcgtccgcgc
      541 agccgcaggg cggcgggatc aactcgacga tgatggtcgc gctgcccggc ggcgcgctcg
      601 ctcccaacaa cagcatcaac gtgcagttcg tcatgggcgt cgaacaaaac ggcagcttcc
      661 gtttcatcgt caacgtcgag gcggcgaccg ctgccgcccc cccgcctgcg ggcgcagcag
      721 gcaagcgggt cggcacgaaa cagggaagcc gttaacgcga agcgagcaag agcagaatag
      781 gccgcccgaa ctttcacggt tcgggcggcc tattcatgcg acgggttgtg tcttctccga
      841 ctacacggaa cgtctaacgt ttcgacgcta gctcgtcggt cgctcggtgg gcgggtggac
      901 ggcgtggaag tctctataaa aacgcgcgga gtagtcgttg agtaaatact ctatgcgtgc
      961 cggatcgtgc gacatgacga tgaggccgac gtgggattgt ttgttcatgc gccagacgat
     1021 ctccgggtcg tcgtaactgg acgtgtccgg agattgctgc cgcgcgaggg agacgagcaa
     1081 gcccgcgtaa ttctcgcggc gcggcggcaa ctcgtacgcg cccggcttgc ctccggcgat
     1141 ctcgaccttc gcccattcag cccagagatt gatgcccgtc gccgcttcga cgagttccac
     1201 gatgtttgcg ccgccgacgc gcgccgaagt ttcgaggaaa tagaacttgc cgtcctcgcg
     1261 ccccttgatg aactccgtgt gcgagacgcc gtgcggcagg cgcatcgcgc tgagcacgcg
     1321 gcggttgatc tcctgcaagg ccacttcgtc cggcgtgccg cgcagctccg tgcgcgtggt
     1381 gaagatgccg ccgccgtgcg ccacgtcgag cggcgggcga ccgtagcggt tgacggcggc
     1441 gaagagcact tccgaattgt agacgatgga atcgacgtgg tagatgtcgc cgggcacgta
     1501 gcgttcgagc acgtggtaac actggcggtt gccgagcgtg tcgagcgcag gccagagttc
     1561 gtccggcgct ttgatctttt tgatgccggt ggcggaagcc tcgaagcgcg gcttcaagag
     1621 ccacggcgca gggacgcggc gcatgtagtc gcgcagccgt tcgtagttga gaacgtgcgc
     1681 aaactccggc acggcgatgc ccgcttcgag agcctgcatg cgcatggcga gcttgtcgcg
     1741 gaagtagcgc gtggtggtgt cgcccatgcc gggaacgcgc aggtgttcgc gcaaggcggc
     1801 ggcggtttcc agatcgaaat cgtcgagcgg aacgatgcgg tcgatctctt ccgtgcgcgc
     1861 aatgtagctg acgctgtgaa ttacgtcttc gagcacccac ttgtgattct ggtcgggcat
     1921 gtagaagatg tcgtcgagac tctcgcgcgg ccagtctgcg tcgcggatgc tatgcgaagt
     1981 gagcaggagg acgcggcagc cctgccgctt gcactcgcgc ataaactcct gacccttctc
     2041 ataactggcg atacagagaa tggtgagcgg acgtaacggg gtcgtcatgc aatacgaact
     2101 cctttatgaa ttccaaaaat cgctggtgat gatgcgccgt gcctcttcga gcgtgagatc
     2161 gccgcgcgag aatttgcagg cgaccgtctc ttcgtaaccg gcgcgtcccg cgcgtgtgcg
     2221 cagggcggcc tgttcgggcg tgatgcctgc tgcttcgagc gtctgcgcgt gtcgcggctc
     2281 gaaacaacgc gcgccgagcc actcttcaac ctcttccgcg tcggtgaatc ccgcttcgag
     2341 ccacgcgcgc gccgccgctt cgacgttcgc gctttgcagc gcgtcgtcgc cataccggcg
     2401 cagcatctcg ccgatgagat ggagaagcat ttgttgtggc ggcggcagcg gcgcagcggc
     2461 gcggtcgttc gtcatcagaa gagtctctac acgtgttcac acggagaaaa atcacggatg
     2521 ggctaatcta tagcgtggac gcgccgcttg ccaagcggag aagggtcgcc gcgcgcttcc
     2581 gtttcggtcg cgtgcgattg cataatgtgg ctctcaagtt tgaacgaact ttcggaagat
     2641 atgactttca ccacggtcgg cgtcgggggc ttcgcgtcaa acgcgggcaa gaccactttg
     2701 atgtgcgaac tgctgcgcgc gctcgaaccc ggttgggagg ccatcaagat cacgcgcggg
     2761 cattatcgtt cgtgcggcaa agacccgcag gcgtgctgcg tgagccatct cctgtcggag
     2821 cagcccgtga tccgttcggg gcgcgccgag acttacgcgg cgggcaaaga cacggggcgt
     2881 tactgggacg ccggggcggc aaacgtccac tggctgatcg tgacggataa gcaggtcgag
     2941 cgcggcatca agtctgcgct ggagcgcgtc cgcgcaaagg gcgtgttgat cgaagggacg
     3001 agttttgtgc agttcgtcga agtcgatttg atgttgatgg cggcgcgtgc ggcgggcgag
     3061 ggaaagatca agccttcggc gcggcgcgcg ctggcgaaga cggacgcgct ctatctcttc
     3121 gacgaagatg cgggggcgac ggagacgcgc gcgcgtttcg ctgcgtcgcc gtttggcgca
     3181 gcgggcgaga atttgaccgg cagcattccg gtctacacgc gcgaagattt gccccgactc
     3241 gtcgagcgtg tcagggagat tcagaggagc ggcgtcgccg cttgaattaa ttaacgagcc
     3301 gagaggagac taaaactatg tcggacaagg atcgtcggaa gaaggacgga gagcgcaagg
     3361 acggggatcg agcggagatg cacgaggccg tcgccgagcg caaggcgggg caggaacccg
     3421 cgcaactgcc gcgcgataag tcgaaccatt tgatcggcga cgatgaaacc gccgagcagt
     3481 tgaaagacga cgcaagttga gactcttata gactctatgg ctgaagggcg cggctacatc
     3541 tatcaactga attgttcgga cggcggcgtg ccgaaactcc ccgtccgtga agcgcaactg
     3601 acgccgaccg gactctctgg cgaccggcaa gcccacccgc gctttcacgg tggacccgaa
     3661 cgcgccctct gtctctacgc gctcgaacag attcatgcgc tacaggcgga agggcatccc
     3721 atctttcccg gttcggtcgg cgagaacgtc accatcgcgg gtcttgattg gccgagcctc
     3781 gcgcccggcg tccgtctggc tttgggcgat gaggtcgtca tcgaactcag ttcctacacc
     3841 acgccttgcc ggagcatcag gcgttcgttc agcggaggga agttcgggcg catctcgcag
     3901 aagacgcacg cgggcgagtc gcgcctctat gcgcgcgtcc tgcgcacggg caaactcgcg
     3961 gtcggtcagg ttgtgcgcgt cctcgacgcc ggagccgccg gatagactcg aaggataaag
     4021 cgaacgtgaa gagcaaccgc agcgcactca tcctcgtcga tatacagaac gatttttgtc
     4081 ccggcggctc ccttgcggtg aaagacggcg accggatcgt gcccgtcgtc aacgaactgc
     4141 aaaagcattt cgatctaatt gtggcgacga aggactggca tccgccggga catgcgagtt
     4201 ttatctcgct ctggccgccg cattgcgtgc aggagacggc gggcgcggag tttgtcgagg
     4261 ggctggacac gagccgcatc gcgcgcgtct ttctcaaagg caccgatccg gagattgatt
     4321 cctacagcgg cttcttcgac aacgaacata agcgcgccac cggactcggc gattacctgc
     4381 aagcgcgcgg cgtcaacgag gtgacaatcg ccggactcgc cacagactac tgcgtgaagt
     4441 tcaccgcgct cgatgccgtc atgctcggct tcgagacttc ggttgtcgtc gatgcctgtc
     4501 gcggcgtgga agtgaacgaa ggcgacaccg cacgcgccgt cgcagagatg tccgccgccg
     4561 gagtgaagat tagggagagc cgccagttgc tcgaagcgca agccgcgaag gagcaggagg
     4621 cttgaaccgg aaacctcttc gagaagattg aagactattc gcgggtcaca ctaaaacccg
     4681 cgcggctttg aacgcggcca gccaacagga ggactccaat atgcagcggc gacgcacttt
     4741 cacgcttctc ctcatgctca acatcatctt gctctcgaac ggatgcacct cagacaccgt
     4801 gcgcccgtcg agcaacggta acaacgcgaa cacggggggc gtcggcaata acagcacggg
     4861 cagccgtgag ggaatcggcc cgaaagacac gggcatcggc ggcacaagcg aaggcaacgc
     4921 caactcggcg gtcaagagca attccaacgt cgaaccgtct ggcatcaaca agaacgccgg
     4981 tcgcggcgca ccggccaaca gcaaccagcc ctaacgcgca cgctataaat ctacggacgc
     5041 gccgcacgca tcacgcgcac gcgatgcggt gcgcgctcac gtcgaataaa aaaacggcga
     5101 ccgggagagt tctccgggtc gccgttcgtg cgttgaagtc gaatgccttt agtgttgaac
     5161 gccttcgatg aaggctttca ggaggcgcga gcgtgaagga tgacgaagtt tcctgagcgc
     5221 cttcgcctcg atttggcgaa tgcgttcgcg cgtgacgctg aaatctttgc ccacctcttc
     5281 gagcgtgtgc tcctcgccca ctggacccag tccgtagcgc atcttgatca ctttctcttc
     5341 gcgcggcgag agcgtcgcca gcacttcgtc cgtgatctcg cgcaggttcg ccgccaccac
     5401 tgagtcagcc ggattcacgc gccccttgtc ttcgatgaag tcgccgaggt gcgaatcttc
     5461 ctcttcgcca atcggcgttt cgagcgagat cggctgctgc gccagtttaa agacgctgcg
     5521 caccttcgag acgggcatgt caaccttctg cgcgatctct tcgctcgtcg gctcgcgccc
     5581 caactcctgc acgagcgcgc gtgaagtgcg gacgagtttg ttgatcgtct cgatcatgtg
     5641 gacggggatg cgaatcgtgc gcgcctgatc ggcaatcgca cgcgtgatcg cctgtcgaat
     5701 ccaccacgtc gcgtaggtcg agaacttgta gccgcgccgc cagtcgaact tctccacgcc
     5761 gcgcatgagg ccgatgttgc cctcttgaat gaggtcgaga aattgcaggc cgcgattcgt
     5821 gtacttcttc gcaatcgaga cgacgagacg caggtttgcc tccgtcaatt cgctcttggc
     5881 ctcgatggtt tcggcgacgc tgcgcgcgat tgattgatgg gagcgtttga tctcaagaat
     5941 ggagacttga tgctcggcct cgatctcttt gagacgcttc ttggccgcgc tgatgtgctt
     6001 tttgaactcc ttctcctgct ccggcttgat gcgtttcgcg tgcagcttgt ccgtgtactt
     6061 ttcgatctcg cgttcggcgg agcgaatgtc tttgtggacg gcggcgatgg cgtcgatgag
     6121 acgttggcgc gtcgcgtctt tcaaagtgag ctgcttcatc tcctgcgcga tctctaagcg
     6181 cgcgcccgcc accttgcgac gcagacgcaa catcttcttg ctgcgcttgc cgcgcgttac
     6241 ctgctgctcg gcgcgaagtt tctcccaatc tcgcaagccc tgctggaaga tcgtgttgat
     6301 cttctcaatg ccttcgagcg tccaacgctg gtactcttcc gtcttgtctt cgaggtctgc
     6361 gacatcggct tgatcggcaa aggcgaccac gtcgcggatg ctgagcatcc cggcgtcgat
     6421 gtcttctccg atttgcaaaa gttctttgag cgcgatgggc gagcgcgcca gagacttgtg
     6481 cgtgcggttc tggccgcgtt cgatgcgctt ggcgatggag acttcctgtt cgcgcgtcag
     6541 cagcgggacg gttcccatct ggcgcaggta ggtgcgcacg gggtcgctcg atttgtcatc
     6601 cacaccggct gacaagtcga gttcgtcttc ggcgtcgagc gggtcgtccg tctcgtctgc
     6661 ggcgacggcg gcgacttctt cgatgagaag ttcatcggag tcggcttcgg tcaggctcgc
     6721 gccgccgagt ttctgtaaaa cgtcttcgag tttgctggtc gaaatggcat cgtcgtgctg
     6781 ctcctcgccg actccgtcga aagaaaggta tttgtcgcca ccaaaaccga ccgggcgaag
     6841 gccatcatcc gctctgtcct tctcaatcat actcaccctt aacctctgcg cctgccgctc
     6901 tttagctcat tgtgcgcgat aagtgccgcc tctacgataa actatttatt aaagccgcgt
     6961 ggcctgtagc ataaactcct tatgcacgcg tggcaacccc cgccgcgcca ccacgttagt
     7021 acgagttaga tgaatttaaa gattaaaaag ttttatcgcg aggggaaatt aacctctttg
     7081 tgtgagggaa gtgaggcgta aatccaacaa atcactggcg aaacggctcc gaaagcgtta
     7141 cgcgcggagc aatcacctgt gagaaacgag tgccctccgg cgcgcgagcg accgtgtgcc
     7201 ggactctgaa aaaaaagtga tgcggggcgt ttggagtcgc cgctaaaaat tccgtttgcc
     7261 gctcggtgcg gcgtggactc ggtgaaaccg cgtctcagga gaactgtatg cgttgccaga
     7321 cggcctgttc gtccggaaaa gagatgtcct cttcgcctct gagcgaactt gccgagacga
     7381 cgattgtttc aacgtcgtga taaacgtcgc agcagggcgg gcaatcgtcc aagtgccgac
     7441 gtatgccgtc gagttgcgtg tcgtcgagcg agccagcgtg gtattcgctg agcagaggca
     7501 aactttcttc acagtccatc gaggtactct ccgggagggt attaaatttt ctggcgcaca
     7561 taagatgcta attgttcgcc gcctgtcgcc tgatgaggtc ccttaatttt aagcgcgcct
     7621 tgtgcagttg cgatttgctc gttcccgtcg aggtgccgag catgcgcccg atctcctcgt
     7681 gctcgtagcc ttccacgtcg tggaggatga agacgctgcg ataaccgggc ggcagttccg
     7741 agatggcttt ctccaacgcg atgcggtcaa cgacgggcat tttgtctgcg ttctccgtgc
     7801 cgcgcacgat ctggacgggc gtttcgccct cgtgcgtgag caactcggag cgcgtgctgc
     7861 tcttgcgaaa atacatcagg acatggttga ccgtcatgcg gtgcagccag gtggtgaagg
     7921 ccgactcgcc gcgaaaactc ccgatcttgt tgaagaggtg gatgaacact tcctgcgtca
     7981 aatcttctgc ggtcgaaggg ttggtcgtca tgcgcaggca gagagcgtag acgcgtcgaa
     8041 agtggcgatg atacaactgc tcgaacgcct cgcggtcgcc cgccgcgctg cgctccgcca
     8101 gttccgcgtc cgacaccttc ggcgtcgttt ccgtcgcctc tttattagtc tctgctgcgc
     8161 tcatgttttg tcccttcgtt gtccgctgat cttcgggcgt gcgcgcccga atgtcaaccg
     8221 cgaccggaca caagttaatt ggaatgtgtg gtgaaagttt tgcggcgcgg cgaggaggac
     8281 gggtataatc gccgcagcaa accgtaagtc tcgtcttcac atcgaaacca tttctccgaa
     8341 gcactacatg aaaacgccaa acacgaaacg cgctctatgt cttctctacg cggctctctg
     8401 tctcgcgtgg gcggtcattc ccctgtgcac gcacgcggag gcggcagcga gcaactggcg
     8461 gcgcgtgcgc tcggagaatt tcgtcgtcgt cagtaacgcg ggtgaagcgg aagcgcgtgc
     8521 gggcgcggcg cgtctggaag aactgcgcgc catctttgcg cggcttcagg gaacggatag
     8581 tttcgacgtg acgcttcccg tcacggtgat cctgtttcaa aacgacggcg attacgaatt
     8641 tttcaaaccg atgtatcgcg gcgacctgcg gcgcgacgtg gccgggtatt ttctgttcag
     8701 cccggacatc aactacatca cgctctcgat ggagacagcg cgcgggcgcg agccgcattc
     8761 cgtgctcttt cacgaatacg tccacgcgct cgtcaggaac aactacgcgg gcgcgccgct
     8821 ctggctcaac gaagggctgg cacagtacta cagcgagttt gagttgtcga aggacaggcg
     8881 gcgcgtaaac ttaggccgcc agctcacgca tcgcctcgac ccgctcgcgc gcggcgaatt
     8941 cctgccgctc gaaacgcttc tctcggttga cgcttcttcc ccgtactacc agcagcgcgg
     9001 caagcatacg gtcttttacg cgcagtcgtg ggcgttcgtc cattatcttt tgagcgacgc
     9061 gagcggcgcg cgccagacgc aactcacgcg ttatctcgaa ctcgtgggcg cgggggcgac
     9121 cgtcgaagag agttttcgcg aagcgttcaa agtcggcttc gggcagatgg agagcgagtt
     9181 gcgcgcctac gtccgcgccg cccattacag ggagcgcagc gaatctttcg cggagcctgt
     9241 gccgcgcagc ctcgatgcga aactggaaag ccgcgcgctc acggaggcgg aggtgcaggc
     9301 gacgctcggc gacctgctcc tgcacgcagg ccgcgaggag gacgccgaag aatatctcac
     9361 acgcgctctg aaacttgatg ccgcgctcgc gtcaggtcat agttcgctcg gcctgctgcg
     9421 agtcaaacag gagcgcttga gcgaggcgaa agagcacctt gagcgcgcca tccgggtcga
     9481 gccagagcat tatctcgcgc gcttctatta cgcagatgtg ctgcgccgcg aaggtctgga
     9541 gacggagaag accgtcacgg gctacgccgc caccacgcgc ctcattcgcg ccgaactcaa
     9601 gaaagtgatc gaactcgcgc ccgacttcct cgaagcctac gcgctcctcg gcctcgtcga
     9661 tctcgaacga agcccgcagg tggaagagac tttcgcgttg ctcagacgcg ccgccgcgct
     9721 cgcgccgaaa cgtcaggagt tcaaactgct cttagcgcaa ctccacctgc ggctcgaagc
     9781 gttcggcagc gcgcggcaga tattggagcc gctcgcgcac gaccgccgtt ccgcacgcac
     9841 gcgtgccgaa gcgcaggcat tgctcgaacg cgtggcgacg aacgaagcgc aggccgcgtt
     9901 gcagcgcagg gcgcagagca tcgaaccgga caacgcaacg acgagcgcag gcgcgaccag
     9961 cggcgcgccg gacgtgaagc ctccgggcga cgccacgaca acgcccctga gcgacgccgc
    10021 gcccgtcggc gtggaggcgt tgccctgcga tatgccgcag ccgggtcccc agtttaagcc
    10081 tctgcgcttc gacgggcagc aggcgtgcgg ccaactcgtc agcatcgagt gtgaagatgc
    10141 gggcggcgtc acgctgtcgg tcgagacgcc ggagcgcact ttgaaactgc gcggccccgc
    10201 gctcaaccgt attcgttttg tcagctacac ggcggacgtg cgcgggcgcg tcgagtgtgg
    10261 actgcgcacg cacgccaacc ccgtgctcgt cacctatcgc ccgctgaagg actcgcgcgc
    10321 agccgccatt gacggcgaag tcgtcgccgt cgagttcgtg ccgccggatt gggttcacta
    10381 aagcgaatcg caatcgagcg ggacttaaac ccttcaccaa ctcacagaga cacagagaac
    10441 acgaaggggg cacggagagt tgaatcatct ccgtgccccc ttcgtgttct cttgtgtctc
    10501 tgcgttgagt gcttcgatca atctctaaaa attctcgacc tcacaacgca cgcgcggctg
    10561 caactctaaa atctcttcca ggctgcacgc gcccgcgccg tgcagtgcgt tggccgctcc
    10621 ggcggcaatg gcgagcgcgc ccgactcctg aactgtccac ccacgacaca gcccggcggc
    10681 gagtccggcc agcgtcgcat ccccgctccc gacgctgttg accgcctcga cctgtggcgc
    10741 gctgaatcta tattgcgctg ctccgaagtc gaacaacgcg ccctgcgcgc cgagcgtgat
    10801 cgacgcagac tccgcgcccg actcgcgcag cttgcgcgcc gctcgcgccg ccccgtcgaa
    10861 ctctgtgacg acgggcaagc ccaaaagttc cgaagcctcc gctccgttga tcttgacgag
    10921 gaaggggcgc gcttcgagtg cgtgccgcag agacgcgccc gcgcagtcga gcaacacgcg
    10981 cttgccgcgc gcgtgcgccg cttcaatcag gcgcgcataa gtctggtcgg gcaagtccgg
    11041 cggcaggctg cccgtgatga cgacgtaacc ggcagcgggc agcgaacgct tgtagcttgc
    11101 ttcgaagctg cgccaattcg cgtcgctgat gctcgcgccc ggagcgttga tgacggtctg
    11161 ctcgcgacgg ctccggtcgt tgatgatgag acatgtgcgg ctctcatttt cgacgcgcgt
    11221 gaagcgcgac gcgatctctt cttcgcgcaa acccgccttg ataagtctgc ccgcgcgccc
    11281 tccgagaaaa cccagcagca gcgacggctc gcccagagtc ttgagcacgc gcgcgacgtt
    11341 gacgcccttg ccgcccgcct gtcgccgctc gccgcgcgaa cgctgcaccc cgcctgcgcg
    11401 cagctcgtcc acttcgacga tgtgatcaac cgcaagattg agattgacga tcaggatcat
    11461 aaattcctgc gactcgcgga cgccttacgc cttacacctt caactcgacg atgcgtgcgc
    11521 ggcaactctt cccgcgcacc gtgagcagcg cgacggagac gggcagcttg aaacggcgcg
    11581 ctcccgcgct tcccggattc agatagagca cgccgtccgt gcgtcgctcg ctcgaagggc
    11641 ggtgcgaatg gccgcacacc acgatgtcga agccggaggc cgaaggatcg agcgtgagtt
    11701 cctgcaaggc gtgcaggagg tagagcctga cctcgccgat ctcgacaatc tcggtttcgg
    11761 cgaacgcttt cgcccaagcc tcctggtcgt tgttgccgcg cacggcgacg acgggcgcga
    11821 ggttgcgtaa cgcgtcgagc acttcggggc gtccgatgtc gcccgcgtgc aggatcaagt
    11881 ccgcgcccgc gagagccttg agggcttccg ggcgcatcaa accatgcgtg tcggagatca
    11941 cgccgatgag atatttgtct ttgggcggca atgcgctacg catgatacct gactgacata
    12001 cttgacagac cctcccgctc cggcatcggc tggcggcgac gcgcacgcac gcgtcccgtt
    12061 cgagcgactg aaaatttgct ttcgcgcgtc attcttccga caagttttgt cgctagaagt
    12121 tcaggcgcag agtttaagga gacggacgtt gacaatggtg aactacatcg atctcttgct
    12181 cgtcgcgatt gtgctcttga gcgcattcta cggttggtat cgcggattca tactcggcct
    12241 gctcgacctg ctgcgctgga tcgggagcct gctgctcggc ttgcgcttct accagccgct
    12301 cgcgcgctgg ctcgccccgc atttcgactg gtcggaagca tgggacagac ccgtcgcctt
    12361 tatcttcacg gcgttcgtcg caggcttggc gattcaactg ctcggcaacg cgctcatccg
    12421 gcgtctgccg aaagatgtgc acgagcggcg tctgaatcgc atcttcggca tcgtgccggg
    12481 cgcggcgagc ggtctgatca ccgccgccat caccgccgcg ctgctgatgg ccgcgccgct
    12541 ccccgacggg ctgcaagcgc gcgcacgcga gagcacgctc gccaatcgtc tcgccgcttc
    12601 aaccgaacaa ctcgaaaccg cgctcgctcc cgtcttcaac gacgccatcg cgcagacgct
    12661 caatcgcctg acggtcaagc cggagtcgaa cgagagcgtc gaactgcctt tcaaagttgc
    12721 cgacacgaag ccgaggccgg agttggaagc cgagatgctc gaactagtca atcgtgaacg
    12781 cgtcgccgcc ggactctcaa ccctcgccgc cgacgacgaa ctcagggaag tggcgcggcg
    12841 tcattcgtca gacatgtttg cgcgcggcta tttctcgcac gtcacgcccg aagggcgcga
    12901 tcccttcgca cgcatacgcg aagcgggcta caccttccgc accgccgggg agaacctcgc
    12961 gctcgccccc acgctctcgc tcgcacacac cgggctgatg aactcgcccg gccaccgcgc
    13021 caacatcctg cgcccgcaat tcggtcgcgt cggcatcggc atcatggacg gcgggcggcg
    13081 cggcctgatg gtcacgcaga acttccgcaa ctgaagaaag ggatgaggga tgagggatga
    13141 gggatgaata agaaaagcac tcaactggct tgaatgctct tcttattcat ccctcatccc
    13201 tcatccctca tccctttttc gtcagaccct ctgcgattcc ggcacgagcg acttgtgtat
    13261 gtggatggcg gccatcgcgc cttcggcggc ggcgacgacg gcaagttgcg acttcggcgt
    13321 gaggtcgccc gccgcataga cgcccggcac gctcgtctgt tgatctgcgt ccacccagac
    13381 cagcccgcat tcttcgtcca tccggcagtc gagcatctcg tgcagagtcg aagcaggctc
    13441 cgtgccgagg ttgaagaaga gggcgtcgca ttcgagcgag tcgccatcct cgaatcgcac
    13501 gtgccgaatc tgtttcgtct ccgcgtcgcc ttccaacttc tcgatgcgtt gattcgtgat
    13561 ggggatgtcg aacccggcca gcttcgcacg atgctcgtcc gtcaggtcg
//
LOCUS       DASWNS010000624        12904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_440576, whole genome shotgun sequence.
ACCESSION   DASWNS010000624 DASWNS010000000
VERSION     DASWNS010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440576"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VGB76_21670"
     CDS             complement(<1..225)
                     /locus_tag="VGB76_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101550.1"
                     /translation="MTAQQQQQEAAARFGGVVDHIAVRCEDLRRDVSEYERIGFRLET
                     MYEDWAMLRDGRGFGIALLPPGSKHPPHMGL"
     gene            complement(332..1024)
                     /locus_tag="VGB76_21675"
     CDS             complement(332..1024)
                     /locus_tag="VGB76_21675"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY0101551.1"
                     /translation="MQDKPTNLPTTQPGGEPLKPYPAAVIERRATTEFLPDEVPEETL
                     NAILQLGAQAPSGYNLQPWRFIVVRGAENRQKLQAVAYNQAKVGQAPVVVIALGMKEE
                     WKRTAEEVFREGAERGAGSTETWRQYMEGALGFLSQMPMEVWVNRHTMIAFTALMYAA
                     EAYGFDTAPMEGFDAAGVRREFGIPEEAEVVALLAIGRAKQPDKVYPGRFPLERIFFS
                     ERYGEAWGSESK"
     gene            1204..2151
                     /locus_tag="VGB76_21680"
     CDS             1204..2151
                     /locus_tag="VGB76_21680"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY0101552.1"
                     /translation="MSRHTPRIVSLLPSATEIVCALGLADSLVGVTHECDYPPEVVGK
                     PQLTASRISHQTLTSAEIDHAVRSQLDSHGSIYDLDEVALHALRPDLVITQELCDVCA
                     VSYKTVEQAAARLFETDVRVVSLEPNTITDIFSNIRTVGGLTGREAEAEQVVGELATR
                     LDALALARSEVQTRPRTFVLEWLEPPFAPGHWVPEQIAFAGGDHAFGQAGRPSTTTTP
                     EEIRAYAPERIVLAPCGYYKEDILRALESARLPEGWGELPAVASGEVWAVDATAYFSR
                     PGPRVVTGAEILARIIHPELFGTPEAHEAVRVPAELMRT"
     gene            2157..3965
                     /locus_tag="VGB76_21685"
     CDS             2157..3965
                     /locus_tag="VGB76_21685"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0101553.1"
                     /translation="MPRKTTKTPNPAQPTAAPESLNASDTGSGGALQQFAQTAEAVAA
                     TTKKLEKAALLGAYFHTLDDADLVRAARYFAGHQFALNDARTTNVGSNLLRDVLTDVT
                     GFDAENLRPRYVRLGDSGDVAYEAMREAGRDEVNQPAFTLAGVESLNALLSETRGTKN
                     KRTLLAEIFAGATALEAKYLVKLLVGDLRIGLKEGLVEDALARAFERKLADVAQVNML
                     TGDIGETAVRARKGDLADVSMRLFHPIKFMLATAGEDLADIARTMPGEFYVEDKFDGI
                     RAQAHTEAGRVALYTRTLDEVTHRFPELVAPLAALKTDAILDGEIVPARGAEILPFAE
                     LQKRLGRKTVGDELLASTPVAFIAYDLLYAAGRVMFDEPLAERRRVLESLLRDSGGND
                     GAGGVVPGSDDGGDDAMTLRLSEAKRMTDVALLDAEFDAARGRGNEGLMIKDPRSSYK
                     PGKRGREWLKLKRALATLDVVVTAVEVGHGKRRNLLSDYTFAVRRSAEDAELLNVGKA
                     YSGLTDVELAELTEWFRAHTLEEFAHGRVRTVEPKIILEVTFDRVQASKRHKSGYALR
                     FPRILRLRGDKSVDEIDTLETVSRLAGESDDGEVIS"
     gene            complement(4156..4932)
                     /locus_tag="VGB76_21690"
     CDS             complement(4156..4932)
                     /locus_tag="VGB76_21690"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEY0101554.1"
                     /translation="MLHENSRRSRLATSRLYVALLGLVIFSLAAQPIYAVGGTFFDGL
                     DTFNTSRWHKADGWTNGNPFNVGWRADHVTFASGFMSQRLDTTPCPSGCSGKPYAAGE
                     YRTNEYYGYGKYETRFKAAKGSGLVTGFFTYTGPSDGTQHHEIDIEILGKDTTKMQTN
                     YFVNGVGGHETIVNLWFDAAAGYHNYAFVWKADKIEWIVDDIIVHTEYASTGNPLPSL
                     PGKIMVNMWAGTGVDSWLGPFTYPGTPIYAKYDWIQYFVL"
     gene            complement(5358..5897)
                     /locus_tag="VGB76_21695"
     CDS             complement(5358..5897)
                     /locus_tag="VGB76_21695"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEY0101555.1"
                     /translation="MSTKTRAEAQPMPVNIGIEAGDRREIAEGLSRLLADTFTLYLKT
                     HNFHWNVTGPMFQTLHLMFEEQYNELWTANDAIAERIRALGLFAPATYREFARLTSIA
                     EEDGVPVATQMIRQLVEGHETAARTARSVITIAERADDAPTADLLTQRLQVHEKTAWM
                     LRSLLAEERPDTVAAAQGA"
     gene            complement(5990..7972)
                     /locus_tag="VGB76_21700"
     CDS             complement(5990..7972)
                     /locus_tag="VGB76_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101556.1"
                     /translation="MIRSTLFADDDSPSSMTNPLERAATTPSVHEPPLRWRWGLLAAL
                     AMMLLSLYPQAYLCYQRGGQWQGSYAHFYSDEPAYSAYVNALIDGRPRRNDPYTGQDD
                     TPDAPLPESLFSIQFVPPYMLSIPARLFGLSASTMFILLMPLIACVSALAIFRLVSLI
                     TRDERMAAAAVPVVLCLGILVSGQGLVLNILRMQTAYVFLPFLRRYVPGVPFAFFFLF
                     CCFVWSALTSAARRTRLVNASLASVLFAAMVYSYFYHWTSAVALLGCLALLWLVARPS
                     DWRRRSVEPFVLMSVCAVVSLVPYFILLSHRAPTMDTVQALTYSRAPDLWRGVILLDA
                     LALSALAFGVWRRWLDARSPAVLFNAALALSVLVVFNQQIITGRSLQPMHYEQYIGNY
                     VALLAATLAAGLLWQGREILKRPGREKLEDGKLEVGTLEDEKLTGARAGGVRRLLPHR
                     VWLPVALAALLWGAGETRIITRRGAVGNVLKDEWAQVALRLKERARGEQPDPHPVIFI
                     PDVYRIDNLPTYAPLATVWAPHMFVFSRVSVAENKERFFKFLYYSGVAANDFETIYRQ
                     QGFIYFAIFGWERANPRLTVNYRPITQAEIDVEKNNYANFHTSFDRERAARPTLSYVV
                     EQVGRPFNFAPIDRWYTRDAGERVGKHIIYRVKLRD"
     gene            complement(8017..9987)
                     /locus_tag="VGB76_21705"
     CDS             complement(8017..9987)
                     /locus_tag="VGB76_21705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101557.1"
                     /translation="MRDERKRPVNGASPIHPASRIPHAPFRWRLGLFAATALMLLALL
                     PQAHLLRARGDNWHGAYASFDGDETAYAAYLSALIEGRPRLNDPYSGLDDTAATPLPE
                     SLFSIQFVPAYALALPARAFGLNTSNVFIVLSPLVAFAASLALFWLLGLLTRDERLAA
                     CGTVVILCLGALASGQVGLASLLSLRAAHTYLPFLRRYVPALPFPLLFIFCAFLWRAL
                     TCAGRQQRRTAHVFALAAGVTFALLVFSYFYLWTTAAAWLACLALLWLSTRTGRDARA
                     HALKIFLTTTALACAALVPYALLLARRAATMDTVQALKFTHAPDLFRAPEMLALVALA
                     LLVAGVWLKRLEAREARVVFAASFALTPFVVFNQQIITGRSLQPVHYEDYVLNYLALV
                     ALVLSAALLSRRRTRSDPSPMPPQEASTQPDLPRRYASTRRFAGMILLGVALAAYGWA
                     ARELIATTRRFAPVNAARDEQMRVASRLAEMARTKTAAMMTNDPAHARPIVLAESLML
                     ADNLPTGAPQTSVLWSPHMHVNSGLTASGHRERMYQFFYYTNVAPENFHAYLAANPLL
                     SYMLFGAERVLPRLTTDHAPVSAAEIDQAARAYADYFAAFTREQATRPALSFVITSHD
                     SPGALSNLDRWYERDAGERVANYTIYRVRLRP"
     gene            10320..12692
                     /locus_tag="VGB76_21710"
     CDS             10320..12692
                     /locus_tag="VGB76_21710"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0101558.1"
                     /translation="MSTTVYEDANAKPSARLAAVCACLLMLVALVDSQVVGAITPQIA
                     LGLNSTKTSVAASVMVYALAAAAVALLLGRRAGRVNPVRWLPAAAGIFVAANALAAIA
                     PHVALFWTARALAGLAGGLVSALVIAALADASSYARRGKQMSGVAVAYFLAPVVGVPL
                     GTWLAGRVGWRAVFAASALLVGVAGLLVRRFPLSTANAAKGEAAAGEFREASGELADD
                     APRPERAEQSERVERTATTGGRTSLWRLAMRSRSTRRGIVSAFFVSGGLVGLTTYLGT
                     WLSDAFRAGTREVGLIYALAGAGAVAGGALGGLLADRYGKRRVAIWSSALMIFLLLVL
                     PTFNWSARLWAVIGATAFLAALRVAPLQALVTELVAASERATYIALRNGASQLGIALA
                     VAAGARLYPHYGLAGVGAMCAALTLGATLSMRKLADPHDVPHDAPRDDSARASTGGDD
                     KRARLVSEGDASRAQIDAGDQSARRRRFSGRSITRKLAAVVILLLLVVTIGFPWLLSF
                     AITKAGTRPDERRRTDTPATHGANFEPVSFTSTDGNTLSGWYLPARDGAGRRVTFILT
                     HGLFRSRYEMLERGLALWRAGYGVLLYDLRRHGASAAEFSTIGFDERHDVAAAYQFIR
                     GRAPDERIVLMGVSMGAAATLLAAAEMQDEKLLCIVAESSFLSFADTARHHVRLARLP
                     TFPFATLLIKFTAWRLNFAAEDYDLVRAARKISRPILFIGGTKDARMPNATVLEPLYA
                     AAASPHKRRYIVEGATHGRAYDQSPDEYVKAVAGFVEAVEAAERAGDSRP"
BASE COUNT         2518 a         3867 c         4274 g         2245 t
ORIGIN      
        1 caagcccata tgcggcgggt gcttcgagcc gggcggcaga agcgcgatgc cgaagccgcg
       61 cccgtcgcgc agcatcgccc aatcttcata catcgtctct aagcgaaacc cgatgcgttc
      121 atactcgctc acgtcgcgcc gcaaatcctc gcaacgcacc gcgatatgat ccaccacccc
      181 gccaaagcgc gccgccgcct cctgctgttg ctgctgtgcc gtcataactc aactccggaa
      241 gaaatgttga acgcggagta atgaatgatg aatggatgaa tggaagccga aagcagttgc
      301 tctgattcat cattccgcaa ttcatcattc atcatttgct ttcgcttccc cacgcttcgc
      361 cataacgctc gctgaagaag atgcgttcga gcgggaagcg accggggtag actttgtcag
      421 gctgcttcgc gcgcccgatg gctaagagcg cgaccacctc ggcctcttcc gggatgccga
      481 actcgcgtct gacgcccgcc gcatcgaaac cctccatcgg cgcggtgtcg aacccatagg
      541 cttccgccgc gtacatcagg gcggtgaagg cgatcatcgt gtgacggtta acccacacct
      601 ccatcggcat ctgcgagaga aagccgagcg cgccctccat gtactgacgc cacgtctccg
      661 tgctgcccgc gccgcgctcc gcgccctcgc gaaaaacttc ttccgccgta cgcttccatt
      721 cctctttcat gccgagcgcg atgacgacga cgggcgcctg cccgaccttc gcttgattat
      781 aagccaccgc ttgcagcttt tgccggttct ccgcgccgcg cacgacgatg aaacgccacg
      841 gctgtagatt gtagcccgaa ggggcttgcg ctccgagttg caagatcgcg ttgagagtct
      901 cttccggaac ttcatccggc agaaactctg tggtcgcgcg gcgttcgatg accgctgcgg
      961 gataaggttt gagcggttcg ccgccgggtt gcgtcgtcgg tagattcgtc ggtttgtcct
     1021 gcatcgcgtg aagtctcccc tggggttcgt gtttgctaca acggtcggtt tcgagattgt
     1081 agcgcgagac gcaggcaaag ggaaaaggcg gcagcgcgcg aaagattctt gacagcagag
     1141 actctcaggc gcgaacctct caggcgaaac tcaatcgacg caaatgagaa aggcaaactt
     1201 ttgatgagcc gtcacacacc gcgcatcgtc tcgctgctgc cttcggccac agagatcgtc
     1261 tgcgccctcg gactcgcaga ctccctcgtc ggcgttacgc acgaatgcga ctacccgccg
     1321 gaggttgtcg gcaaacctca actcaccgcc agccgcatct cgcaccagac tttgacgagc
     1381 gcggaaattg accacgccgt ccgctcccaa ctcgacagcc acggctcgat ctacgacctc
     1441 gacgaagtag ccttgcatgc gctccgcccc gacctcgtca tcacgcagga actctgcgac
     1501 gtgtgcgccg tcagttacaa gacggtcgag caggccgccg cccggctctt cgagacggat
     1561 gtgcgcgtcg tctcgcttga accgaacacc atcacggaca tcttctcgaa catacgcacc
     1621 gtcggcggtt tgaccggaag ggaagcggaa gccgaacagg tcgtcggcga actcgccacg
     1681 cgcctcgacg cgctcgccct cgcccgctcc gaagttcaga cgcgcccgcg caccttcgtg
     1741 ctcgaatggc tcgaacctcc tttcgcaccc ggccactggg ttcccgaaca gatcgctttc
     1801 gccggaggcg accacgcgtt cggccaggcg gggcggcctt ctaccacgac gacgccggaa
     1861 gagatacgcg cctacgcgcc cgaacgaatc gtgctcgcgc cctgcggtta ttacaaagag
     1921 gatattctgc gcgcactcga atcggcgcgc ctgcccgaag gctggggaga gcttccggca
     1981 gttgcgagcg gcgaagtatg ggcggtggat gcgacggcgt atttcagtcg tccgggtcca
     2041 cgcgttgtca ccggcgcgga gatactcgcg cgtatcattc acccggaact gttcggcaca
     2101 cccgaagcgc acgaagccgt gcgcgtgcct gcggagttga tgagaactta aacgagatgc
     2161 cacgcaaaac gacgaagacg ccgaaccctg cacagccaac agccgcccct gaaagtttga
     2221 acgccagcga caccgggagc ggcggcgcac ttcaacagtt cgcgcagacc gcagaggccg
     2281 tcgcggcgac gacgaagaaa cttgagaagg ccgcgctgct cggcgcgtac ttccacacgc
     2341 tcgacgatgc agacctcgtg cgcgccgcgc gctacttcgc cggacaccag ttcgcactca
     2401 acgacgcgcg caccacgaac gtcggctcca acctcctgcg cgacgtgctg acggacgtga
     2461 ccggattcga cgcggagaat ctgcgcccgc gctacgtacg cctcggcgat tccggcgacg
     2521 tggcctacga agcgatgcgc gaagccgggc gcgacgaagt taaccaaccg gccttcacgc
     2581 tcgccggagt cgaaagcctc aacgcacttc tgagcgagac gcgcggcacg aagaacaaac
     2641 gcacactgct ggcagaaatc ttcgcgggcg cgaccgcgct ggaagcgaag tatctcgtca
     2701 aactgctcgt cggcgacctg cgcatcggtc tgaaagaggg tctggtcgaa gacgctctgg
     2761 cgcgtgcgtt cgaacgaaag ctggcagacg tggcacaggt gaacatgctt acgggcgaca
     2821 tcggcgagac ggctgtgcgc gcgcgcaagg gcgatctggc ggatgtctcg atgcgcctct
     2881 ttcacccgat caagttcatg ctcgcaaccg ccggggaaga cctcgcggac atcgcgcgca
     2941 cgatgcccgg agagttttac gtggaagaca aattcgacgg catccgcgcg caggcgcaca
     3001 cggaagcggg acgcgtcgcg ctctacacgc gcacactcga cgaggtgacg catcgcttcc
     3061 ccgaactcgt cgcgccactc gcagccctca agaccgacgc catcctggac ggcgaaatcg
     3121 tgcccgcacg cggcgcagaa attctgccct tcgcggaatt gcagaagcgt ctggggcgca
     3181 aaacggtcgg cgacgaactg ctcgcctcaa cgcccgtcgc cttcatcgcc tacgatctgc
     3241 tctacgcggc ggggcgcgtg atgttcgacg agccgctcgc ggagcgtcgc cgcgtgctcg
     3301 aaagtttgct gcgtgatagc ggcggcaacg acggtgcggg cggtgtcgtg cctggtagcg
     3361 atgacggtgg cgatgatgcg atgacgctga ggctctcgga agcgaagcgc atgacggatg
     3421 tggctctgct cgacgcggag tttgacgcgg cgcgcgggcg cggcaacgag ggcttgatga
     3481 tcaaagaccc gcgttcgtct tacaagccgg ggaagcgcgg gcgcgaatgg ttgaagttga
     3541 agcgcgcgct ggcgacgctc gatgtcgtcg tcacggcggt cgaagtcggc cacggcaagc
     3601 ggcgcaatct gctgtccgat tatacgttcg ccgtgcggcg ttcggcggag gatgcggaac
     3661 tgttgaacgt cggcaaggct tattcgggtt tgacggatgt ggaactggcg gaactcaccg
     3721 aatggtttcg cgcgcacacg ctcgaagagt tcgcgcacgg gcgcgtgcgc acggtcgagc
     3781 ctaagatcat cctcgaagtc accttcgacc gcgtgcaagc ctcgaagcgg cacaagagcg
     3841 gctacgccct gcgcttccca cgcatcctgc gcctgcgcgg agacaagagc gtcgatgaga
     3901 tcgatacgct ggagacggtg agccgcctcg cgggcgagag cgatgacgga gaagttataa
     3961 gttgagatga atgtcgagag agcggtgagc cacgacgctc agtgagcggt gagccgcgac
     4021 gctcagtgag acgatgcgtg aggatagtgt tgcgaaactt attcacggcg cacgattatg
     4081 ttgacggggc gagccgtctc acacgccctg ccgtcaacgg aatatttttc gcaacactac
     4141 ctcaaacgct accctttaaa gtacgaaata ctgaatccaa tcatacttcg cgtagatggg
     4201 tgtgcccgga tacgtaaacg ggccgagcca actatcaacg cccgtccccg cccacatgtt
     4261 caccatgatc ttgcccggca ggctcggcag cgggttgccc gtcgaggcat actccgtatg
     4321 cacgatgatg tcgtcaacta tccactcgat cttgtccgcc ttccagacga acgcgtaatt
     4381 gtgataaccc gccgccgcgt cgaaccacag attgacaatc gtttcatgac cgccgacgcc
     4441 gttgacgaaa tagttggtct gcatcttagt cgtatcttta cccagaatct cgatgtcgat
     4501 ctcgtggtgt tgcgtgccgt cggatggacc tgtgtaggtg aaaaagcctg tgacgagtcc
     4561 gctccccttc gcggctttga agcgggtttc gtatttgccg tagccgtaat actcgttggt
     4621 gcgatactcg cccgcggcgt aaggcttacc gctgcacccg gaagggcagg gcgtggtatc
     4681 gagccgctgg ctcatgaagc cgctggcgaa ggtaacgtgg tcggcgcgcc agccgacgtt
     4741 gaatgggtta ccgttagtcc agccgtcggc cttgtgccag cgtgaggtgt tgaacgtgtc
     4801 gaggccgtca aagaaagtgc cgcccacggc atagatcggc tgcgcggcca gcgaaaagat
     4861 aaccagaccc agcaaggcaa cgtatagacg cgatgtagcc agacgggagc ggcgagagtt
     4921 ttcgtgaagc atgaagtgtc ctccaattgg tgaagtaagt aagttgaggg agaacgaacc
     4981 gaaacagtga gggatgacac gagacgcacg ggcatggcga accctgtgcc agagcggaag
     5041 agacgggcga ttgcccttat aaaaggctga aaacggtatg acgacaaccg gcaggcgaag
     5101 ccgtctgagc atcgcttcgt tacagttcgg attaaaagcc tacagcttgt gaagagtccg
     5161 gtgaggaacg atgtgtgcgg gcgattaact gcggcatagg ctttcgcgcc tgacgaaccg
     5221 tagaagctgc tttaccggag tgagatcatt cttcgcggtt cacctctccc ggttaagact
     5281 aaggccgttt cgctctgcac cacagagaag cgaaacggcc ttagtcttat gcaggggcaa
     5341 aattcacccg cacggtttta cgcgccctgc gcggcggcga ccgtgtcggg acgctcttcg
     5401 gccagcaggc tgcgaagcat ccacgcggtc ttctcgtgga cttgcaggcg ttgcgtgagc
     5461 aggtcggcgg tcggcgcgtc gtcagcccgt tcggcgatgg tgatgacgga acgcgcggtg
     5521 cgcgccgccg tctcgtgtcc ttcgacgagt tgacggatca tctgcgtggc gacgggcacg
     5581 ccgtcctctt ccgcgatgga ggtgaggcgc gcaaactccc tgtaggtggc gggcgcgaag
     5641 aggccgagcg cgcggatgcg ttcggcgatg gcgtcgttcg ccgtccagag ttcgttgtat
     5701 tgctcctcga acatcaggtg gagcgtttga aacatgggtc ccgtaacgtt ccagtgaaag
     5761 ttgtgcgtct ttaagtagag cgtaaaagta tctgcgagca ggcgcgacaa accttccgcg
     5821 atctcgcgcc tgtccccggc ttcgatgccg atattgacgg gcatcggctg agcttcggct
     5881 ctcgttttag ttgacatgaa attctctcct tctatcggga gagactacgc cacattcttg
     5941 tcggtcgttt gtccaagtgt tctgcattat ctcgcgcgtt cgcggcgcat cagtcgcgca
     6001 acttgacgcg gtagatgatg tgcttgccga cgcgttcgcc cgcatcgcgc gtgtaccagc
     6061 ggtcgatggg cgcgaagttg aaagggcgtc cgacctgttc gacgacgtag gagagcgtcg
     6121 ggcgtgcggc gcgttcgcgg tcgaaggagg tatggaagtt ggcgtagttg ttcttctcca
     6181 cgtcaatctc ggcctgcgtg atgggacggt agttgacggt caggcgcggg ttggcgcgct
     6241 cccagccgaa gatggcgaaa tagatgaagc cctgctggcg gtagatggtt tcaaagtcgt
     6301 tggcggccac gcccgaatag tagagaaact tgaagaagcg ttccttgttc tccgcgaccg
     6361 agacgcgcga gaagacgaac atgtgcggcg cccacacggt ggcgagcggc gcgtaggtcg
     6421 gcagattgtc gatgcgatag acatcgggga tgaagatgac cgggtgcggg tcgggttgct
     6481 cgccgcgcgc acgctctttc agacgcagtg caacctgcgc ccattcgtct ttcaatacgt
     6541 tccccaccgc gccgcgccgc gtgatgatcc gggtttcgcc cgcgccccac aaaagcgcag
     6601 ccagcgcaac gggcaaccag acgcgatgcg ggagcaaacg tcgcaccccg cctgctctcg
     6661 ctcccgttaa cttctcatct tccaacgtgc caacttccaa ctttccatct tccaactttt
     6721 ccctgcccgg gcgttttaaa atctcccgac cctgccagag caagcccgcc gcgagggtcg
     6781 ccgccagcag cgcgacgtag ttgccgatgt actgttcgta gtgcatcggt tggagcgaac
     6841 gccccgtgat gatctgttga ttgaagacga cgagcacgga gagcgcgagc gcggcgttga
     6901 agaggacggc ggggctgcgc gcatcgagcc aacgtcgcca cacgccgaac gcgagcgcag
     6961 agagcgcgag cgcgtcgagc aggatcacgc cgcgccagag gtctggcgcg cgtgaatagg
     7021 tcaacgcctg caccgtgtcc atcgtcggcg cgcggtgcga gagcagtatg aaatagggca
     7081 cgagggagac gacggcgcag acgctcatta agacgaaagg ttcgacgctg cggcgtcgcc
     7141 agtcagaggg tcgcgcgacg agccagagga gcgcgaggca gccgagcagc gcgacggcac
     7201 tcgtccagtg ataaaaatat gaatagacca tcgccgcaaa gagcacgctc gcaagcgaag
     7261 cgttgacgag tcgcgtgcgg cgcgcggcag acgtgagcgc gctccagacg aagcagcaaa
     7321 agagaaagaa gaaggcgaac ggcacgcccg gcacgtagcg gcgcaggaag gggagaaaga
     7381 cgtaagccgt ctgcatcctc aaaatattca agaccaagcc ctgaccggag acgaggatgc
     7441 cgaggcacag cacgacgggc acggcggcgg cggccattcg ttcgtcgcgc gtgatcaacg
     7501 agacgagccg gaagatggcg agcgcggaca cacacgcgat gagcggcatg agcaggatga
     7561 acatcgtcga ggcactgagg ccgaagaggc gcgccggaat cgagagcatg taggggggca
     7621 caaactggat cgagaagaga gattcgggca ggggcgcgtc cggcgtgtcg tcctgtccgg
     7681 tgtacgggtc gttgcgacgc gggcgtccgt caatcagcgc gttgacgtaa gcggaatagg
     7741 ccggttcgtc gctgtaaaaa tgcgcgtagg agccttgcca ttgcccgccg cgttgatagc
     7801 agagatatgc ctgcgggtag agcgagagca gcatcattgc cagcgcggcg agcagacccc
     7861 aacgccagcg gagcgggggt tcgtgtacgg acggagtggt tgctgcgcgt tcgagcggat
     7921 tggtcataga ggagggcgaa tcgtcgtctg cgaataacgt tgaacgaatc aaaaaacgtc
     7981 tgatactaag attgagagac gacggagagc aagccttcaa gggcgcagac ggacgcgata
     8041 gatcgtgtaa ttggcgacgc gctcgcccgc gtcgcgctcg taccagcggt cgagattgga
     8101 gagcgcgccg gggctgtcgt gcgaagtgat gacgaaggag agcgcagggc gcgtggcctg
     8161 ctcgcgtgtg aaggctgcga agtagtcggc gtaagcgcgc gcggcctgat cgatctctgc
     8221 ggcggagact ggcgcatggt ctgtcgtcag gcgcggcagg acgcgctccg cgccgaagag
     8281 catgtaactc aagagcgggt tggcggcgag gtaagcgtgg aagttttcgg gcgcgacgtt
     8341 cgtgtagtag aaaaattgat acatgcgctc gcgatgcccg ctcgccgtga ggccggaatt
     8401 gacgtgcatg tgcggcgacc agaggacgga agtttgcggc gcgcccgtgg gcaggttgtc
     8461 ggccagcatg agactctccg cgaggacgat ggggcgcgca tgcgccggat cgttcgtcat
     8521 catcgccgcc gtctttgtgc gcgccatctc ggcgaggcgg gaagcgaccc gcatctgttc
     8581 gtcgcgcgct gcgttgacgg gcgcgaacct gcgcgtggtt gcgatcaact cgcgcgccgc
     8641 ccagccgtaa gccgcgagcg cgacgcctag aagtatcatt ccggcgaagc gtcgagttga
     8701 agcgtagcgg cggggcaagt cgggttgcgt cgaagcctct tgtggcggca ttggcgacgg
     8761 gtcgcttcgc gtgcgtcgcc gggagaggag cgcggcggac aggacgagcg cgacgagcgc
     8821 gagatagttc aacacgtagt cttcgtaatg gacgggctgg agcgaacgcc ccgtgatgat
     8881 ctgttgattg aagacgacaa acggcgtgag cgcgaatgag gcggcgaaga cgacccgcgc
     8941 ctctctcgct tcgagacgct tcagccacac gcccgcgaca agcagggcga gcgcgacgag
     9001 cgcgagcatc tccggcgcgc gaaagagatc gggcgcgtgc gtgaacttga gcgcctgcac
     9061 cgtgtccatc gtcgcggcgc ggcgcgcaag gagcagcgcg tagggcacga gcgcggcgca
     9121 ggcgagcgca gttgtcgtga ggaaaatttt caaggcgtgc gcgcgcgcgt cgcgcccggt
     9181 gcgtgtggag agccagagga gcgcgaggca ggcgagccat gcggcagcgg tggtccagag
     9241 ataaaaatag gagaagacga ggagggcaaa cgtcacgccc gccgccagcg cgaaaacgtg
     9301 cgccgtgcgc cgctgctgcc gccccgcaca cgtgagcgcg cgccagagaa acgcgcagaa
     9361 gatgaagagc agaggaaatg gcagcgcggg gacgtagcga cgcaggaaag gcaggtaggt
     9421 gtgcgccgcg cgcaggctca ggagcgacgc gaggccgacc tgtcctgacg cgagcgcgcc
     9481 gagacacagg atgacgacgg ttccgcacgc cgcgagtcgt tcgtctctcg tgagcaaccc
     9541 gagcagccag aagagcgcga gcgaggcggc gaaggcgacc agagggctga gcacgatgaa
     9601 gacgtttgaa gtgttgagtc cgaaggcgcg cgccggaagc gcgagcgcgt aagccgggac
     9661 aaactggatg gagaagagcg attcgggaag cggcgtggcc gccgtgtcgt caagcccgct
     9721 gtaaggatcg ttcaagcgcg ggcggccttc gatgagcgcg ctcagataag cggcataggc
     9781 tgtttcgtcg ccgtcgaaag aggcgtatgc gccgtgccag ttgtcgccgc gtgcgcgcag
     9841 gaggtgcgcc tgcggaagca gcgcgagcag catcaaggcg gtggccgcga acagtccgag
     9901 acgccagcga aacggggcat gcgggatgcg agacgcggga tgaatcggag acgctccgtt
     9961 aaccggacgc tttctttcat ccctcattcc tcgtccctca ttcctgttct tcaacgcgcg
    10021 ctcccggcgt ctgcgtgcct gcggcggcga gtggctttcg tcataactca agggcaacgg
    10081 ttacaagacc gctgattata acttctcttc gcaggccgac gcgcgcagaa gagggcgcgt
    10141 ggaagattaa cgacgcgaca agcccccttg cttgccgcgc gcaggttaaa gttgacaagt
    10201 agtgtgacgc aagcgaagat gcgagacaac cttgcaaaga gttgtttcga gcgttctacc
    10261 gggagaagtt cacaagctaa gttcacaaga catgctgttg atttaagccg acgcttttta
    10321 tgtcaacgac tgtttacgaa gacgctaacg cgaagccttc ggcgcgcctc gccgccgtgt
    10381 gcgcgtgcct gttgatgctc gtcgcactcg ttgactcgca ggtggtcggc gcgatcacgc
    10441 cgcagatcgc gctcgggtta aacagcacga agacgagtgt ggccgcgagc gtgatggtct
    10501 acgcgctggc agcggcggcg gtcgcgctgc tgctcgggcg acgcgcggga cgtgtcaatc
    10561 cggtgcgctg gctgcccgcc gccgccggaa tcttcgtggc ggccaacgcg ctcgcagcca
    10621 tcgcgccgca cgtcgcgctc ttctggacgg cgcgcgcgct ggccggactc gcgggcggac
    10681 tcgtgagcgc gctcgttatc gccgcacttg cagacgcttc gagttatgcg cggcgcggca
    10741 aacagatgag cggcgtggcg gtcgcctatt ttctcgcgcc cgtcgtcggc gtgccgttgg
    10801 ggacgtggct cgcggggcgc gtgggttggc gcgcggtctt cgccgccagc gcgttgttgg
    10861 tcggcgtggc ggggctgctc gtgcggcggt ttccgctgtc aactgcaaac gcagcgaagg
    10921 gcgaagcggc ggcgggcgag tttagagagg cgtcgggcga acttgcagac gacgcgccgc
    10981 gaccggagcg cgcggagcaa tcagagcgtg tcgagcgcac ggcgacgacg ggcgggcgca
    11041 cgtctctatg gcgactcgca atgcgttcgc gctcgacgcg caggggcatc gtgagcgcgt
    11101 tcttcgtctc cggcggactc gtcgggctga cgacttatct cggcacgtgg ctctcggatg
    11161 cgtttcgcgc gggcacgcgt gaggtcggtt tgatctatgc gctggccggg gcgggcgcgg
    11221 tggcgggcgg cgcgctcggc gggttgctcg cagaccgtta cggcaaacgg cgcgtcgcga
    11281 tctggagcag cgcgctcatg atctttttgc tgctcgtgct gccgaccttc aactggagcg
    11341 cgcggctgtg ggcggtcatc ggcgcgacgg cctttctcgc cgcgctacgc gtcgcaccgc
    11401 tgcaagcgct cgttacggaa ctggtcgccg cctcggagcg cgccacttac atcgctctgc
    11461 gcaacggcgc gtcgcaactc ggcatcgcgc tcgcggtcgc ggcgggcgcg cggctctatc
    11521 cgcattacgg gctggcgggc gtcggcgcga tgtgcgccgc cctcacgctc ggcgcgacgc
    11581 tcagcatgcg caaactcgcc gatccgcatg atgtcccgca tgatgccccg cgcgacgact
    11641 cagcacgcgc gagcacgggc ggcgacgaca aacgcgcacg gctcgtgtcc gaaggcgacg
    11701 cgagccgtgc gcagattgat gcgggcgacc agagcgcgcg gcggcgtcgt ttcagcggca
    11761 ggagcatcac gcgaaagttg gcggcggtcg tcatcttgct cttgctcgtc gtcacaatcg
    11821 gctttccgtg gctcttgagt tttgcgatca cgaaagcggg gacgcgcccc gacgaacgca
    11881 ggcgaacgga cacgcccgcc acgcacggcg caaacttcga gccggtcagt ttcacttcaa
    11941 ccgacggcaa cacgctttcc ggctggtatc tgcccgcgcg cgacggagcg gggcggcgcg
    12001 tgactttcat cctcacgcac ggactctttc gctcgcgtta cgagatgctc gaacgcggcc
    12061 tcgccctgtg gcgcgcgggt tacggcgttt tgctctacga cctcaggcgg cacggcgcga
    12121 gcgcggcgga gttttccacc atcggcttcg acgagcggca cgacgtagcg gcggcatatc
    12181 aatttatacg cgggcgcgcg ccggacgagc gcatcgtgct gatgggcgtt tcgatgggcg
    12241 cggcggcgac gctgctggcg gctgccgaaa tgcaggacga aaaactcctg tgcatcgtcg
    12301 ccgagtcgag ctttctctct ttcgcggaca cggcgcggca tcacgtccgg ctggcgaggc
    12361 tgccgacttt tcctttcgcg acgctattga taaagttcac cgcatggcgt ttgaacttcg
    12421 cggcggagga ttacgatctc gtgcgcgcgg cgcggaaaat ctcgcgcccc atcctcttca
    12481 tcggcggcac gaaagacgcg cggatgccga acgccaccgt gctcgaaccg ctctacgcgg
    12541 cggctgcgag tccgcataaa cgcaggtaca tcgtcgaagg cgcgacgcac gggcgcgctt
    12601 acgaccagtc gccggacgaa tacgttaaag ctgtggccgg gtttgtcgaa gccgtcgaag
    12661 cggcggagcg cgccggagac tcgcggcctt gaaaccgtgc gcgcgtcctg cacgtatcaa
    12721 atagctattc aagcggaaat tgtcatagcc aaaattgatg aaaatggaat caaaagacca
    12781 tctccatcaa gatcaactcc ggcgaccgcg cgctgaccga gaaactgacg ctctggtaaa
    12841 tttctcgcac gagttgccaa ccgacaggag aactaagatg agtgaaaaga atccggcgga
    12901 gacg
//
LOCUS       DASWNS010000625        20672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_233292, whole genome shotgun sequence.
ACCESSION   DASWNS010000625 DASWNS010000000
VERSION     DASWNS010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20672
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233292"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VGB76_21715"
     CDS             <1..550
                     /locus_tag="VGB76_21715"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0101559.1"
                     /translation="NAVSNTYTLNLGYGSGVTAKGTGVLLNNEMDDFAAKPGTPNAFG
                     LIQGERNAVAPRKRPLSAMTPTFVLRKNGTLWFTIGTPGGPTIINTVLQAIINIVDHD
                     MDIQQAIDSPRIHHQWLPDEIVHEPYGLSADTQRALAARGHKLVSRPRYMGDAHGIMI
                     EEKTGMRLGASDARNEGAAVGY"
     gene            764..2434
                     /locus_tag="VGB76_21720"
     CDS             764..2434
                     /locus_tag="VGB76_21720"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY0101560.1"
                     /translation="MFIKRVSFSLLAMLLLLSLAGGSLRPVNSQNSAAGAATAIRVTP
                     PAPVFDDQARLAELAARRARVAQEIGKQSLLVLFSGQPRVYTNDVDYEFRQENNLFYL
                     TGLKQQRATLVLIPGHPATPEILFIPRRNPAAETWTGHMYSPEEARAISGITEIWEAT
                     EFEPFMRALRARRGYRPKDEAILLSNVAPPAPTPTPVAGSTAATPATPVPQNAGGYEA
                     LFGAMAQKNGTLYLLIPGGEGDDREFRQEQAFAARWAQSSSGLNVRDAWPIFMEMRLR
                     KSASELQLLQHAIDITVEALGRAMAVAGRSRWEYEVEAEVDYTFKRRHADNWGYPSIV
                     GCGPNATTLHYQESEGAIGANELLLMDVGAEYSHYTADVTRTFPISGKFTAAQADIYN
                     AVLAAQEASMRAARPGNTFADVNRASIEVIRDALLRMGLITDRNSFQYARWFMHGTSH
                     WLGMNVHDVGALNTKFEPGMVFTIEPGIYIRPDALDNLPKTEESRKFIEAVRPAFEKY
                     KGIGVRIEDDVVITADGHRNMSAALPRTLADVENFMARAAREVRASKN"
     gene            complement(2547..4772)
                     /locus_tag="VGB76_21725"
     CDS             complement(2547..4772)
                     /locus_tag="VGB76_21725"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0101561.1"
                     /translation="MISLDEKIRTTETTAGARAGELEVEVAGEGAGRKKSPVIWIQLV
                     AFVFGLALLIYVVRHVGVQSIFDTLAQIGLGFFILIGISGLRHFFRAVSMRFAVPPDH
                     RRFNLWQAFATRLSGEAISFLTFTGPLLGEATKAALLRKRVPLVTGVQALVVDNLLYH
                     LSVALFILSGACVMLASYNLPEAAHYTLLLIATLAMLTLVAFGLAAARRVMPLTWFIE
                     RLAAVGFKPRALAKRRAHISDLESNVYDFYRHHRGSFFAIVAFDLIAHTSSVLEVFLT
                     LRMLGFHPGLGAPYVIESLTKVINFVFGFVPATIGVYEGGTEIILRTLGFAAATGVTL
                     GLVRKAGMIFWTGIGLSMLATRTLPVATQRVIERHPRLQKAMDNLVLSNIAHRPARTL
                     VSIAGIAVGVLLIVFTVGLAHGVLRERGRREAGVGAEIMLRASGTVGLSGSQPFAMPV
                     AHASEIARIEGVRAAVPIGQVTGKTGSGFGSRIIDGINFEQYQNLSGLKIIEGRALGA
                     SGDEVIVDTEWNRERKKRVGDTEPIYDRPFRIVGIYEPPAGPRLKIPLATMQEEVGST
                     ASCTSILVACTNPAEQDAVAERIHQAFPDDQLIFTRDLPELYASGVPALNVFISVIVA
                     IAAAISMLVILLAMYTTVTERTRQIGILKSLGMSNAMIAWVIEQEALLVSVIGVLVGL
                     VLAFGARFALMRTSSLSVEIEPRWVLLSLLIAVVGGSIGALYPALRAARQDAVDALSY
                     E"
     gene            complement(4769..7393)
                     /locus_tag="VGB76_21730"
     CDS             complement(4769..7393)
                     /locus_tag="VGB76_21730"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY0101562.1"
                     /translation="MAPSGSNYYASRALLQTTRRPACSLLHHASMLFVCVCLLLAAKT
                     SFAQARRLVLVKVDGLSQTMVDRFVRERDPRTGKSLLPWFEHIFYERGTRLANFYTRG
                     MSLSAPSWSLIDTGQHAQIKGNVEFDRLTLHSYDYLNFIPFWLGTAGKVRVDMPGPEL
                     LDELGIPLLVDSYPYDERHISFQLYQRGTRWTTLERGLKNRFLTRTPRELFDEWLVEF
                     EGRNIVMEQLEREMLEKLKDPRLRYLDFYTTEFDHAAHHNRDRETHLVALQELDRILG
                     RIWTTIQQTPQASETAVVVVSDHGTNTDERIYSQGFNLVKLLGSRAGGGHHVITKRRL
                     MSDYALKSIYPLVPLITTTTEDSFYLKGESTAYPTALVDFDGNERASIHLRHSDLNVL
                     HILLTELQSKRLSPARTRAAIDTFFQVIEEHRTDWQKLHTHLLEELGALRRLIEREHA
                     LAAEQPKKWTKADADAGRDQVARRINARVDSWATDERKYAQYAATLSNLLSLKRENFD
                     ASRLRIPDLIAKGAMGEQNTLRDLQNYTVGLQTGGLTLAPDGSLDRDRSFARVNYLAL
                     LRESKVRNNVQADVDSHPVDFVALRVPAEQLFDAQGKDLRADEAVWLYRSEENQALIL
                     VRHGRGGELLLRYVPVAHLRQRPDGSIQYERIPLRAGLPLKIFEDARLLLPANERREN
                     WLDGWHSDLDWLRALHLTTYSNGLVGLHEQLGRHLAPAMEADVPGLSPDEQLIRRFRR
                     RQRLLVETDLLILANNHWNFDVRGFNPGGNHGSFFRISTHSTLMLAGGERTRIPRALV
                     IREPYDSLSFVPTILALTNQLALPDEISPAKFDAPATRPYPGRIINELFDAKQPPARP
                     LTDTAGTGERTRKTEAAP"
     gene            complement(7453..9504)
                     /locus_tag="VGB76_21735"
     CDS             complement(7453..9504)
                     /locus_tag="VGB76_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101563.1"
                     /translation="MKNPISNARARQTACIILVLFLLLHGASPLAVAQDTADSAASTS
                     APAQTPQAGFRLERTPVSGGAELLTVFGSLDGLTQDRAADVPLVSILRDTLGDDAPEN
                     DRLRYVWMHTYTRPSIGQRAASAVPFLYSRVANNKRARTDSPPPHILDLAATDRDVWH
                     RVFWNALQTLVFNPYSFTLKATTHAYRRNADDYRKAHIIRALAVLTIYQTESGAGEVF
                     APHELKEIQARLMLSQKTFGGLIDDLYLARVYEKQTSQWLDTRGHNWELLRQRAEAEN
                     LYFEPLELPDGNTTHALLWIARADVSAARSRRFDKRFLNLANPFGDKRLLRWRGFTET
                     RYFDAQNRRVAAETPGARAVELIPLALYGLDHPKIPIMLVDFRDTLNPKAREMSQRVL
                     QDVARATINLTRLDLPYFLGRAVFDFVSERRGMDVNQPSRLRAYSQLKLLLALNASLD
                     PHLREELSARLERVSLNPLENDMEAESRLARAQYAALLDYARRPDKGLNARLERDRRA
                     ELTPLVHSRPERILLRVANVASFGLYTHREYVAPEKQRATLDTARKLAHHRQFLREVA
                     RSSAAVEVQWKIEDVRRSLSFINEHGERADGKTAEAVGRIFAHTKDEETQKLCLSSLY
                     RINNETAKNTLVRIYRDEAADARWRTLSAQYLRQAKRESQRISTSDARIIATISAMEG
                     Q"
     gene            complement(9801..11528)
                     /locus_tag="VGB76_21740"
     CDS             complement(9801..11528)
                     /locus_tag="VGB76_21740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0101564.1"
                     /translation="MSATTAATAVTANIRPEPYTGQSVRVLSRAFIIAVLSVIVVAGF
                     GFRVSGLGAEGLSEDELNKLRAVAEYRTKGLTSTNGEHPFLMKALMTVSIVAAEKWNE
                     ATPVATSDGSSTARRVSEETALRLPATIVGAFTSLLIYLVVSELFGKATGLIAAALWA
                     FDPAGIGFNRIAKEDSFLLFFFLLANVFWLRGQRVAERGGARPEKYYWITAACFGAML
                     ASKYMPHLLAISAAYYYVFQGIPATRWRMGKRKWLIFFAVMGAAFLVCNPTILLPGTW
                     REMRIFAGEQRIGHDAYEFMGQLYRNQMTLWLKGSPWYFYYAFMAFKLPLTVMAAFLV
                     GLPLLLSKRLGDGRYFVFFWLFFWFFPFPLLGGKFTRYFTMALPVVLITAAIGINAVG
                     LWLARMSARIAANPTLRGYVQACVAALFIASSAYASAGAVPHYRLYTNVLGGGDERAG
                     SYFPHDEFYDASMSETARHIATLARPGARVATEAPELFTHYATRAGRTDLVSISLSDR
                     AALQGLTNGDVIVVARGRRYFSNDALISKLGATSQPVVELALGGVPSTRIYVLDEAAL
                     RVVSEVTGR"
     gene            complement(11846..13504)
                     /locus_tag="VGB76_21745"
     CDS             complement(11846..13504)
                     /locus_tag="VGB76_21745"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0101565.1"
                     /translation="MASGLVVHVEFGAERHTEVLLHERIRIGSDADCELRFQPGAIPR
                     ADGFLLELRRTNGLYRIGDFDRSLRLTHNDEPLAPGAAIKDGDMVRIPETDFVLQFFP
                     VAQPPALVANRRADIHVAPFIESAAIESAATARRDDAKVFLREFTRELIREINLSTKL
                     IALVIVVALVGGFLYFAFAGFKELKQSRRHIEEQNAQIARLNQKVGESQNQFEEVNRS
                     NKDIINSLSLAPKLRSEYGNGVCLISGMYIFVENGTGRPLRYPQRPAATAETDAETES
                     AEVVEGDAQSEALTPEGTGAIAEFPYVGTGFHVGGGYVLTNRHVGVEPWVADERSMVL
                     SSTVSGKPRLTKLMAYFPGHRQAFPLKFRQASTRDDLAVLSFDTGANPNNVPVLPIDD
                     NSDAAGVGKAVIMMGYPSGPDRILVSLPDEEANAIRQRFGSSIDVLVNHLAERNLIKP
                     FTTQGHITDSQTRRVIYDASTAEGGSGAPLFGQSGRVIGVNFGIFEGLPNANFAVPVR
                     FAVPLLERAGWKPAAPPTENTTAAAGETVIKDARPANAPQNQPR"
     gene            13791..14201
                     /locus_tag="VGB76_21750"
     CDS             13791..14201
                     /locus_tag="VGB76_21750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEY0101566.1"
                     /translation="MKEISFIEDKDQVCALAPVAYAPIRLHVLVCSGKSCTSVGSAEV
                     KEAFERELKARQLLFGKAAKGKNPQGSIVLTECASVGFCAVGAAVLVYPDGVWYAQVR
                     ARDVPEIIEEHLLGGRVVERLALLKLPAATAETA"
     gene            14406..15839
                     /locus_tag="VGB76_21755"
     CDS             14406..15839
                     /locus_tag="VGB76_21755"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0101567.1"
                     /translation="MFRFSRKVLRISFAVLVALLLVPQTPKAQTKVPRLDYRERTLQN
                     GLRVLSAVDKSSPTVAIQVWYKVGSKDDPEGRSGFAHLFEHIMFKSTKNMKSEMMDRL
                     TEDVGGMNNASTADDVTIYYEVVPSNYLETLLWAEADRLASLNVDDANFKSERDVVKE
                     EYRERVDANPFGKLFYILMQEKSFTRHPYKRPGIGNIAELDAAAIADVRAFHSTFYRP
                     DNATLVVVGDFDQKQFDAWVDKYFAPIPKPNQPLPRVTVKEPERTGETRIVEFAPNVP
                     LPAVAFSFLAPPKASEDSDALRIAESILSSGESSRLYRALVYEQQVAAQVSAGMDLRE
                     DVSLFQFFAIVAGGKKPDDAERALLAEIKKMQDAPVSAAELEKAKNQIITNQLRERET
                     NNGKALALGDAAVLLGDPNRANTDIERLSKVTAADVQRVVKKYLTDTNRLVIYYLPES
                     LRPAQPAAEKTGQTSAPRDAREAGGGK"
     gene            15841..17385
                     /locus_tag="VGB76_21760"
     CDS             15841..17385
                     /locus_tag="VGB76_21760"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0101568.1"
                     /translation="MSTSKELSRRTLELRALKLGTLKLLALAALFASSAFNAAAQQAA
                     PQAAAQATPPPPAAPRSVTVPKPVERTLPNGLRVVVVERSNMPLVSAAFVIKNGGEVD
                     PPQLAGVADLTASLLTKGTATRTAPQIAEAIEALGGSIESGARWDASTASVGVMSSRI
                     NPAMEILADVVRRPTFKDEEIERLRQQYLDDLTVALGQPGSIARYVASRVVFGDAPYG
                     HPLSGTPESIARIKRDDIVRMHATYYRPDNAILVIGGDIRPEEAFKTAERFFGDWQKP
                     AASLPAPGAKATAAATTGASAQPRIVVIDKPDAGQAAVLVARTGIRRTDPEFFRGIVA
                     NSVLSGYSGRLNQEIRIKRGLSYGARSALETRRDTGPFMAEAQTKNESGAVVAGLLLG
                     ELSRLANGELPEAELTPRKAVLIGGFARNLETADGLVAQVANLALYGLNFEEINNYIN
                     NVQTITAKDVQGFAGSRLDSRTANVIIVGDARKFLPELQKQYKNVEVIPVAELDLNIA
                     QLRKAK"
     gene            17565..18224
                     /locus_tag="VGB76_21765"
     CDS             17565..18224
                     /locus_tag="VGB76_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101569.1"
                     /translation="MPSKLTLLTLKPLALLSCALIVLFTFVADARVLQPQQDDAVNEK
                     PANVSILMRRLTDRDAFVRQRAAEELARLADTRQRKLVEGYRLQEKHPRVRLALDWAL
                     YRMGKPDTLYTVVRDLDSTRYNQSEAYLAELETPEPLYAILPRVNGNTQIKLLEALAR
                     AGNADTLEIVRPYTESPDPKIADAARFATREITRRLDSPQPPDAKTRTRQTGNVPADI
                     P"
     gene            18420..20195
                     /locus_tag="VGB76_21770"
     CDS             18420..20195
                     /locus_tag="VGB76_21770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY0101570.1"
                     /translation="MHRNLRSFLDQLTRERDLVVVETEVDPYLELAEIHRRVIAQGGS
                     ALLFKRVKGSRYPVVTNLFGTARRIELAFGKKPESLVREVVHVVESMLPPRPSELWQH
                     RALALEAFKLGTRRVREAPVLEVVDRPARLDELPILTTWQEDGGAFVTLPLVYTEHPR
                     TRKHNLGMYRIQRYDAATTGIHWQIHKGGGFHYHEAEAANESLPVTIFLGGAPALMLS
                     AIAPLPEDVPELVLASVLAGEKIRVTRDLFTHGHRLVAEAEFAIAGRVPPKERRPEGP
                     FGDHYGYYSLQHDYPVFHAEAVCHRRDAIYPATVVGKPRQEDFFIGDYLQELLSPLFP
                     LVMPSVRDLWSYGETGFHSLAAAVVRERYAREALVSGFRILGEGQLSLTKFLLLTDTP
                     QDLRDFPRLFEHILARARFESDLFIFSNVSMDTLDYTSGKVNQGSKAMLLGLGEAVRE
                     LPREFRGQLPAKVTRAEVFCGGCLVVAGEPYASDPQQAARLATEEAFADWPLVVLHDD
                     ASVARSSADFLWATWTRFEPASDIYAARTSVERHHLSYRPPVVIDARMKPNYPDELVV
                     RPDIAALVDRRWREYFPSSDLVKDS"
     gene            20228..>20672
                     /locus_tag="VGB76_21775"
     CDS             20228..>20672
                     /locus_tag="VGB76_21775"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0101571.1"
                     /translation="MSENQSVETFETSCCIVGGGPAGALLALILARQKLPVVLLEAHE
                     DFERDFRGDTLHPSVLEILDELGLAERLHTFRHSKIHTATFMTPSGALTAADFRRLNT
                     RFPYIMLLPQAHFLEFITGEAKQYPNFQLRMGARVEELIEEAGGVR"
BASE COUNT         4054 a         6272 c         6479 g         3867 t
ORIGIN      
        1 caacgccgtg tcgaacactt acactctgaa tctcggctac ggttcgggcg ttacagccaa
       61 aggcacgggc gtcttgttga ataacgagat ggacgacttt gccgcgaagc ccggcacgcc
      121 gaacgccttc gggctgattc agggagagcg aaacgccgtc gccccgcgca agcgtccgct
      181 gtcggccatg acgccgacct ttgtgctgcg caagaacggc acgctctggt tcaccatcgg
      241 cacgcccgga ggccccacga tcatcaacac ggtgcttcaa gcgatcatca acatcgtaga
      301 tcacgacatg gacatccaac aggccatcga ctcgccgcgc attcatcatc aatggctgcc
      361 cgacgagatc gtccacgagc cttatggact ctccgccgac acgcagcgcg cgcttgccgc
      421 acgcggccat aaactcgtca gcaggccgcg ctacatgggc gacgcgcacg gcatcatgat
      481 cgaggagaag accggcatgc gtctcggcgc gagcgacgcg cgcaacgaag gcgcagcggt
      541 cgggtattga aagcgacgag aaaacagtga cgagtgacag gtggcgtgtg acgagccgga
      601 aactaattcg cgcgtgaatc gtaagctctg tgtctcctct tttgcgcctt ttcgtaaggc
      661 ttggacttgc ggggaacaaa ctcgcgcgag acgcgaaggc gcacagggaa gaagtttgga
      721 cggtagcgga aataagctct gagttgaatg aggtttgcct gtaatgttta tcaagcgtgt
      781 gagttttagt ctgttggcaa tgctgctcct gctctctctg gcgggcggga gtttaaggcc
      841 ggttaattcg cagaacagcg cggcgggtgc ggcgaccgcg atccgcgtca cgccgcccgc
      901 tccggtcttc gacgatcaag cacgtctcgc cgaactcgcc gcgcgtcgtg cccgtgtcgc
      961 gcaggagatc gggaagcaat ccctgctcgt gctcttcagc ggccagccgc gcgtctatac
     1021 gaacgatgtc gattatgaat tccggcagga gaataatctc ttttacctga ccggactcaa
     1081 gcagcaacgc gcgacgctcg ttctcatccc cggccacccc gcgacgcctg agattctgtt
     1141 catcccgcgc cgcaaccctg cggctgaaac ctggacaggc cacatgtata gcccggagga
     1201 agcgcgcgcc atctccggca tcacagagat ttgggaggcc acagagttcg aaccgttcat
     1261 gcgtgcgttg cgcgctcgca ggggatacag gccgaaagat gaagcgatcc tgttgtcgaa
     1321 tgtagccccg cccgcgccca ccccgacgcc cgttgccggg agcactgctg cgacgcccgc
     1381 gacgcctgtg ccgcagaatg cgggcggcta cgaggctctg tttggcgcga tggcgcaaaa
     1441 gaacggcacg ctctatctgc tgattccggg cggggaaggc gacgaccgcg agttcaggca
     1501 ggagcaggcg ttcgctgcgc gctgggcgca atcgtcgagc gggttgaacg tgcgcgatgc
     1561 gtggccgatc ttcatggaga tgcggctgcg caagtctgcc tcggagttgc aactgctgca
     1621 acacgccatt gacatcacgg ttgaggcttt gggtcgcgcg atggctgtgg ctggccgctc
     1681 gcgctgggag tatgaagtag aggccgaagt tgactacacg ttcaagcggc ggcatgcgga
     1741 caactggggc tacccgtcca tcgtcggctg cggcccgaac gcgacgactc tgcattatca
     1801 ggaatcggaa ggagccatcg gcgcaaacga actcctcttg atggacgtgg gcgcggagta
     1861 ctcgcattac acggctgacg tgacgcggac gtttcctatc agcggcaagt ttacggcggc
     1921 gcaggcggac atctacaacg cggtgctggc ggcgcaggag gcttcgatgc gcgccgcgcg
     1981 tccgggtaac accttcgccg atgtcaaccg ggcttccatc gaagtgatca gggatgcgct
     2041 gctgcggatg ggattgatca ccgaccgcaa ctcgtttcaa tatgcgcgct ggttcatgca
     2101 cggcacgtcg cactggctcg gcatgaacgt gcatgatgtc ggcgcgctca acacgaagtt
     2161 tgaaccgggc atggtcttca ccatcgaacc cggcatctac atacgccccg acgcgctcga
     2221 caatttgccc aagacggaag agagccggaa gttcatcgaa gcggtgcgcc cggcgtttga
     2281 gaagtacaag ggcatcggcg tgcgtatcga ggacgacgtg gtgatcactg cggacggcca
     2341 ccgcaacatg tccgccgcgc tcccgcgcac gctcgccgac gtggaaaact ttatggcacg
     2401 cgccgcacgc gaagtgcgcg caagtaaaaa ctaaaaacac acatcactac agagacgcag
     2461 agagatagca cagcgcgggc gcagagagtt taaatcttct ctgcgcccgc gttgtgctat
     2521 ctctctgtgt ctctgtagtg agcttcttat tcgtaactca acgcgtcaac cgcatcctgc
     2581 cgtgcggcgc gcagcgcagg atagagcgcg ccgatgctgc cgccgacgac ggcgatgagc
     2641 agcgagagca agacccagcg cggctcgatc tcgacgctca aactgctggt acgcatcagc
     2701 gcgaaacgcg ccccgaatgc cagcaccagc cctacgagaa cgccgatcac gctgaccagc
     2761 aacgcttcct gctcgatcac ccaggcgatc atcgcgttcg acatgccgag cgacttgagg
     2821 ataccgatct ggcgcgtgcg ctccgtcacc gtcgtgtaca tcgcaagcag gataacgagc
     2881 atactgatgg cggcggcgat ggcgacgatc acgctgatga agacgttgag cgcgggcacg
     2941 ccggaggcgt acaactccgg caggtcgcgc gtgaagatga gttgatcgtc gggaaacgcc
     3001 tgatggatgc gctcggccac ggcgtcttgc tctgccgggt tcgtgcaggc gacgaggatg
     3061 ctcgtgcaac tcgctgtgct gcccacctct tcctgcatgg tggcgagcgg tatcttgaga
     3121 cgaggcccgg cgggcggttc gtagatgccg acgatgcgaa acgggcggtc gtagatcggc
     3181 tccgtgtcgc ccacacgttt ttttcgctcc cggttccact ccgtatcgac gatcacctcg
     3241 tcgccgctcg ccccgagcgc gcgcccttcg atgatcttca ggccggacag gttttggtat
     3301 tgctcgaagt taattccgtc gatgatgcgc gagccgaagc cgctgccggt tttacccgtc
     3361 acctgcccga tggggacggc tgcgcgcacg ccctcgatgc gcgctatttc cgacgcgtgt
     3421 gcgacgggca tggcgaacgg ttgcgagccg ctcaagccca ccgtgccgga agcgcgcagc
     3481 atgatctccg cgccgacccc ggcttcgcgc ctgccgcgtt cacgcaaaac tccgtgcgcc
     3541 aagcccaccg tgaagacgat gagcaggacg ccgacggcga tcccggcgat gctgaccagt
     3601 gtgcgcgccg ggcggtgtgc gatgttggag agaaccagat tgtccatagc tttttgcaga
     3661 cgtgggtgtc gttcgatgac gcgttgcgtc gccaccggca gcgtgcgcgt ggcgagcatc
     3721 gagaggccga tgcccgtcca gaagatcatc ccggctttac ggacgaggcc gagcgtcacg
     3781 cccgtcgcgg cggcgaatcc gagcgtgcgc aggatgatct ccgtgccgcc ttcgtagacg
     3841 ccgatggtcg cgggcacgaa gccgaacacg aagttgatca ccttcgtcag tgattcgatc
     3901 acgtaaggcg cgccgaggcc gggatgaaaa ccgagcatgc gcagcgttaa gaaaacttcg
     3961 agcacgctcg acgtgtgggc gatgagatca aaggcgacga tggcgaagaa agagccgcga
     4021 tgatgacggt aaaaatcgta gacgttcgat tcgagatcgc tgatgtgcgc tctgcgtttg
     4081 gcgagcgcac gcggcttgaa accgacggcg gcgagccgtt cgataaacca cgtgagcggc
     4141 atgacgcggc gcgcggcggc gaggccgaag gcgacgagcg tgagcatggc gagcgtggcg
     4201 atcaggagca gcgtgtagtg cgccgcctcc ggcaaattgt aggaggcgag catgacgcac
     4261 gcgccgctca ggatgaagag cgcgaccgac aggtgataga gcagattgtc tacgacgaga
     4321 gcctgcacgc ccgtcacgag cgggacgcgc tttctcagga gcgcggcttt cgtcgcttcg
     4381 ccgaggagcg gtccggtgaa ggtgagaaac gagatcgctt cgccgctcag acgcgtcgca
     4441 aaggcttgcc agagattgaa gcggcgatga tcgggcggga cggcgaagcg catcgagacg
     4501 gcgcggaaaa aatggcgcag cccgctgata ccaatcaaga tgaagaagcc caaaccgatc
     4561 tgcgcgaggg tgtcgaagat ggattggacg ccgacgtggc gtaccacgta gatgagcagt
     4621 gcgagtccga acacgaaggc cacgagttga atccagatga ccggactctt cttgcgcccc
     4681 gcgccttcac ctgcgacttc aacttcaagt tcgcccgcgc gcgcgcccgc cgtcgtctca
     4741 gtggttctga tcttttcgtc gagcgagatc acggcgcggc ctccgtcttg cgggttcgct
     4801 cgccggttcc cgccgtgtct gtcaacgggc gtgcgggagg ctgcttcgcg tcgaatagct
     4861 cgttgatgat gcgaccggga taaggccgcg tggcgggcgc gtcgaatttc gcgggcgaaa
     4921 tctcgtcggg aagcgcgagt tgattagtga gagctagaat tgtcggcacg aagctcaagc
     4981 tatcatacgg ctcgcgtatc accaatgcgc ggggaatgcg cgtgcgctca ccgcccgcga
     5041 gcatgagcgt ggaatgggtg gagatgcgga agaacgaacc gtgattgccg ccggggttga
     5101 agccgcgcac gtcgaagttc caatgattgt tggcgagaat caagaggtct gtctcgacga
     5161 gtagacgttg acgacgacga aaacggcgaa tgagttgttc gtccggggag aggccgggca
     5221 cgtcggcttc catggccgga gcgagatgtc gcccgagttg ttcgtggagg ccgacgagac
     5281 cgtttgaata ggtcgtcagg tggagcgcgc ggagccaatc gagatcgcta tgccagccgt
     5341 cgagccagtt ctcgcgccgt tcattcgcgg gcaggagcag gcgcgcgtcc tcgaatatct
     5401 tcaaaggcaa cccggcgcgc aggggaatgc gctcgtactg gattgagccg tcggggcgtt
     5461 gccgcaaatg cgcgacgggc acgtagcgca acaacaactc gccgccgcgc ccgtggcgca
     5521 cgagaatcaa cgcttgattc tcttcgctgc gatagagcca gacggcctcg tccgcgcgca
     5581 aatcttttcc ctgcgcgtcg aacaattgtt ccgccggaac gcgcagggcg acgaaatcca
     5641 ccgggtgact gtcaacgtct gcctgcacgt tgttgcgcac cttcgattca cgcagcagcg
     5701 cgaggtagtt gacgcgtgca aaactcctgt cccggtcgag cgagccgtca ggcgcaagcg
     5761 tgagtcctcc cgtttgaagc ccgaccgtgt aattttgcag atcgcggagc gtgttctgtt
     5821 cgcccatcgc gcctttggcg atgaggtctg gaatacgcag gcgggaggcg tcgaaatttt
     5881 cgcgcttgag cgagagaaga ttcgagagcg tcgcggcata ttgcgcgtac ttgcgttcgt
     5941 cggttgccca cgaatcgacg cgcgcgttga tgcgacgcgc aacctgatcg cgcccggcgt
     6001 ccgcatcggc tttcgtccac ttcttcggct gctctgcggc gagcgcatgc tcgcgctcga
     6061 ttagacgccg gagcgcgccg agttcttcca gcagatgcgt gtgcaacttc tgccagtcgg
     6121 ttctgtgctc ttcgatgacc tgaaagaaag tgtcaatcgc ggcgcgcgtc ctggcgggcg
     6181 agagtctttt gctttgcagt tcggtgagca gaatgtgcag gacgttgagg tcgctgtggc
     6241 gcaggtggat cgaggcgcgt tcgttgccgt cgaaatccac gagcgcggtc gggtaagcgg
     6301 tgctctcacc cttgaggtag aaactatctt ccgtcgtggt cgtgatgagg ggcacgagcg
     6361 ggtagatgct cttgagcgcg taatcgctca tcagacgccg cttcgtgatg acgtgatgac
     6421 cgccgcccgc gcggctcccc agcaatttga cgagattgaa gccctgacta tagatgcgct
     6481 cgtctgtgtt cgtcccgtga tccgagacga cgacgacggc ggtttccgac gcttgcggcg
     6541 tctgttggat ggtcgtccag atgcgcccga ggatgcggtc gagttcctgc aaggcgacga
     6601 ggtgcgtttc gcggtcgcgg ttgtgatgcg cggcgtggtc gaactcggtc gtgtagaaat
     6661 cgagataacg caggcgcggg tctttcagct tctccaacat ttcgcgctca agctgctcca
     6721 tgacgatgtt gcgcccttca aattccacca gccactcatc gaacaactcg cggggcgtgc
     6781 gcgtcaggaa acgatttttg aggccgcgtt cgagcgtcgt ccagcgtgtg ccgcgctggt
     6841 agagttgaaa gctgatgtgg cgttcgtcgt agggataaga atccacgagg agcgggatgc
     6901 cgagttcgtc gagcagttcg ggaccgggca tgtccacgcg caccttgccc gccgtgccga
     6961 gccagaaggg aatgaagttg agatagtcgt aagagtggag cgtcagccgg tcgaactcga
     7021 cgttcccttt gatctgcgcg tgttgtcccg tatcaatgag cgaccatgaa ggcgcggaga
     7081 ggctcatccc gcgcgtgtag aaatttgcga gtcgcgtgcc gcgctcataa aagatgtgct
     7141 cgaaccacgg gagcaggctc ttgcccgtgc gcgggtcgcg ctcgcgcaca aagcgatcaa
     7201 ccatcgtctg cgataagccg tcaactttaa ccaagaccag acggcgagcc tgcgcgaaag
     7261 aggtcttcgc ggcgagcaga agacacacgc agacgaagag catcgaagca tggtgaagca
     7321 gagagcaagc ggggcggcgc gttgtctgca aaagggcgcg agaggcgtag tagtttgaac
     7381 cggatggagc catccggcga cacagactgc gtccgcagca ttttattgcc tgccccttat
     7441 ccatcggctc gtctactgtc cttccatcgc cgagatggtg gcgatgatcc tggcgtcgct
     7501 cgtggagatg cgttgcgatt cgcgcttggc ctgccgtaga tattgcgcgc tcaaggtgcg
     7561 ccagcgcgcg tctgcggctt cgtcgcgata gatgcgtacg agagtgttct tcgccgtctc
     7621 gttgttgatg cgatagagac ttgagagaca gagcttttgc gtctcctcat ctttcgtgtg
     7681 cgcgaagatg cgtccgacgg cctccgccgt cttgccgtcg gcgcgttcgc catgctcgtt
     7741 gatgaacgac agcgagcggc gcacgtcttc gatcttccat tgcacttcga ccgccgcact
     7801 cgaacgcgcc acttcgcgca aaaattgacg atggtgcgcg agcttgcgcg ccgtgtcgag
     7861 cgtggcgcgt tgcttctccg gcgcgacgta ttcgcggtgc gtgtagaggc cgaagctcgc
     7921 cacattggcg acgcgcagga ggattcgttc ggggcgcgaa tgaacgagcg gcgtcaactc
     7981 ggcgcggcgg tcgcgttcga gtctggcgtt gagtcccttg tcggggcggc gcgcgtaatc
     8041 gaggagcgcg gcgtattgcg cgcgtgcgag ccgcgactcc gcctccatat cgttttcgag
     8101 cgggttgagc gagacgcgtt cgagccgcgc ggaaagctct tcgcgcagat gcggatcgag
     8161 cgaagcattg agggcgagca ataatttcag ttgcgaatag gcgcgcaggc gcgaaggctg
     8221 gttcacgtcc atgccgcgcc gctcggagac gaaatcgaag acggctcgac cgagaaaata
     8281 ggggaggtcg agccgcgtga gattgatcgt ggcgcgcgcc acatcctgca agacgcgctg
     8341 cgacatctcg cgcgccttcg ggttcagggt gtcgcggaag tcaacgagca tgatcgggat
     8401 tttcggatga tccagaccgt agagcgcgag cgggatcaac tcgacggcgc gcgcgcccgg
     8461 agtttccgcc gcgacacgac gattctgcgc gtcgaagtat ctcgtctccg taaagccgcg
     8521 ccagcggagc agtcgtttgt cgccgaaggg attggcgagg ttgaggaaac gtttgtcgaa
     8581 gcggcgcgag cgcgcggctg acacgtcggc gcgtgcgatc cagagcagcg cgtgcgtggt
     8641 gttgccgtcg ggcagttcga ggggttcgaa gtataaattt tcggcttcgg cgcgttggcg
     8701 caaaagttcc cagttgtgac cgcgcgtgtc gagccattgc gaagtctgct tttcatagac
     8761 gcgcgcaaga tagagatcat cgatgaggcc gccgaacgtt ttttgcgaca gcatgaggcg
     8821 cgcctgtatc tccttcaact cgtgcggcgc gaagacctcg cctgcgcccg actccgtttg
     8881 atagatcgtg agcacggcga gcgcgcggat gatgtgcgcc ttgcgatagt cgtccgcgtt
     8941 gcgccggtaa gcgtgcgtgg tggctttgag ggtgaaactg taaggattaa agacgagcgt
     9001 ttgcagcgcg ttccagaaga ccctgtgcca cacgtcgcgg tcggttgcgg cgagatcgag
     9061 tatgtgcggt ggcggtgagt ctgtgcgcgc gcgtttattg ttggcgacgc ggctgtagag
     9121 aaaaggcacg gcggaagcgg cgcgctgccc gatggaggga cgcgtgtagg tgtgcatcca
     9181 gacgtagcgc aggcggtcgt tctccggcgc atcgtcgccg agcgtgtcgc gcaggatgct
     9241 gacgagcgga acgtctgccg ctctgtcctg cgtgagtccg tcgagactgc cgaagacggt
     9301 caggagttct gcgccgccgg agacgggcgt gcgttcgagc cggaagcccg cctgtggcgt
     9361 ttgcgccgga gcggatgtgg aagcggcgga atcggctgtg tcttgcgcga cggcgagagg
     9421 cgatgcgccg tgcaacagga gaaagaggac gaggatgatg caggcagttt gccgcgctct
     9481 cgcgttcgaa atcgggtttt tcatgccttc gtgcgtgtcc tgcttttgtt cacaaacctg
     9541 ctccttctgg agcatctcga ctgcaccttc ccggcgcgaa tcttcatcaa gacgcggctt
     9601 tcgctttcgt caaacgatgc gtgcgacact tcgcaaaaat gaatggcaac cgatgtgccg
     9661 taagcggggg agagaaagtt ggcaggctat cgctgttgta acattgacgc gccagcgagg
     9721 gcaaacgttt acaagtgcgc gctgcaaaat ttgctcccag acattttctc gtacgccgtt
     9781 catcgcggtt ctcacaacct ctaacgcccc gtaacctcgg agacgacgcg caaagcggct
     9841 tcgtcgagca cgtagatgcg cgtcgaggga acgccgccga gcgcgagttc gacgacgggt
     9901 tggctcgtcg cccccagttt cgagatgagc gcgtcgttgc tgaaataacg ccgcccgcgt
     9961 gcgacgacga tcacgtctcc gtttgtcaaa ccttgtagcg cggcgcggtc ggagagcgag
    10021 atcgagacga ggtcggttct cccggcgcgc gtggcgtagt gtgtgaaaag ttcgggtgct
    10081 tccgtcgcca cgcgcgctcc ggggcgtgcg agtgtggcga tatggcgtgc ggtttcgctc
    10141 atgctcgcat cgtaaaactc gtcgtgtggg aaatagcttc cggcacgctc gtctccgccg
    10201 ccgagcacgt ttgtgtagag acgataatgc ggcaccgcgc ccgccgaggc gtaagccgat
    10261 gaagcgatga agagcgcggc gacgcacgcc tgaacgtagc ctctcaaggt tggattcgca
    10321 gcgatgcgtg cggacatacg tgcgagccaa agccccacgg cgttgatgcc gatggccgcc
    10381 gtgatgagca cgacgggcag agccatcgtg aaatagcgcg tgaacttacc gccgagcaga
    10441 gggaagggaa agaaccagaa gaagagccag aagaagacga agtaacgccc gtctccgagt
    10501 cgcttcgaca acagcagcgg cagcccgacc agaaacgccg ccatgacggt cagcggaagt
    10561 ttgaaggcca tgaacgcata gtagaaatac cacggcgagc ctttcaacca gagcgtcatc
    10621 tggttgcgat agagctgtcc cataaactcg taggcgtcgt ggccgatgcg ttgttcgccc
    10681 gcgaagatgc gcatctcgcg ccacgtgccg ggcagcagga tcgtgggatt gcagacgaga
    10741 aaggccgcgc ccatgacggc gaaaaagatg agccacttgc gcttgcccat gcgccagcgc
    10801 gtcgcgggga tgccttgaaa gacgtagtag taggccgcgc tgatggcgag caggtgcggc
    10861 atgtatttcg aggcgagcat cgcgccgaag caggcggcgg ttatccagta atacttttcc
    10921 ggtcgcgcgc cgccgcgttc ggccacgcgc tgcccgcgca gccagaagac gtttgccagc
    10981 aggaagaaga agagcaggaa gctgtcttct ttggcaatgc ggttgaagcc gatgcccgcc
    11041 gggtcgaacg cccacaacgc tgccgcgata agacccgtcg ccttgccgaa caactccgag
    11101 acgacgagat agatcaggag cgacgtgaac gctcccacaa tcgtagcggg caggcgcaac
    11161 gccgtctctt ccgagacgcg ccgcgcggta ctgcttccgt ctgacgttgc caccggcgtc
    11221 gcttcattcc acttctcggc ggcgacgatg ctcaccgtca tcaaggcttt catcaaaaac
    11281 ggatgctcgc cgttggtcga agtcaaaccc ttggtgcgat attccgcgac ggcgcggagt
    11341 ttgttcaact catcttcgct caacccttcc gcgccgaggc cggagacgcg aaagccgaag
    11401 cccgccacga cgatgaccga gagcacagcg atgatgaaag cgcggctcaa cacacgcacg
    11461 ctctggcctg tgtagggttc gggtctgatg ttcgccgtga cggcggtggc agcagttgta
    11521 gcggacaaaa gtttagtcga actccaatgg gaaaattgtt cagccgggaa gcgacgccgc
    11581 acgcacgcgc tttttgcttc tcgccgcgca cacgcccttc acgcaaaagg cgaaagatta
    11641 cttcggtctg cgcggctctg tcaaaatggc agcccgccgg agtttataga cgatgcgcgc
    11701 aacaagcctc tccgttatga cactttctcg attgtcactg cgagaaagga taaaggcgga
    11761 acgtcgaacg atgaactgaa tacacatagt ctctcattca tcgttcaacg ttccgccttc
    11821 accggtgttt gagtcgcagc caatttcaac gcggctgatt ctgcggcgcg ttggccgggc
    11881 gcgcatcttt aataacagtc tcgcccgctg ccgccgtcgt gttctcagtg ggcggcgcgg
    11941 cgggtttcca cccggcgcgt tcgaggagcg gcacggcgaa gcgcacgggc acggcgaagt
    12001 tcgcattcgg caagccctcg aagataccga agttgacgcc gatgacgcgc cccgactgcc
    12061 cgaagagcgg cgcgcctgag ccgccctcgg cggtgctcgc gtcgtagata acgcggcgcg
    12121 tctgcgagtc tgtgatgtgc ccctgcgtcg taaacggttt gatcagattg cgctcggcga
    12181 ggtgattgac gagcacgtcg attgacgagc cgaaacgctg cctgatggca ttggcctctt
    12241 cgtccggcag cgagacgaga atgcgatccg gtcccgaagg gtagcccatc atgatcacag
    12301 ccttgcccac gcccgccgca tccgagttat cgtcaatcgg gagcacgggg acgttatttg
    12361 ggttcgcgcc cgtgtcgaac gagaggacgg ccagatcgtc gcgcgtggaa gcctgtctga
    12421 acttcagagg gaaggcttgc ctgtggccgg gaaagtaggc catcaatttc gtcaggcgcg
    12481 gcttgccgct cacggtgctg ctcaagacca tcgagcgttc gtcggccacc cacggttcga
    12541 ccccgacgtg gcggttcgtc aacacgtagc cgccgccgac gtggaagccc gtgccgacat
    12601 aaggaaactc cgcgattgcg cccgtgccct caggcgtcaa cgcctcgctc tgcgcgtcgc
    12661 cttctacaac ttccgcgctc tccgtttcag cgtcagtctc cgccgtcgcg gctggccgct
    12721 gcgggtagcg caacgggcgg cctgtgccgt tctcgacgaa gatgtacatg ccggagatca
    12781 ggcagacgcc gttgccgtat tcgctgcgaa gtttgggcgc gagcgaaagg ctgttgatga
    12841 tgtctttgtt ggagcggtta acttcctcga attgattctg cgattcgccg accttctgat
    12901 tcagccgcgc gatctgcgcg ttctgttctt cgatgtggcg acggctctgc ttcaactctt
    12961 tgaacccggc gaaagcgaaa tagaggaacc cgccgacgag cgcgacgacg atgacgagcg
    13021 cgatcagttt cgtgctcagg ttgatctcgc gtatcagttc gcgcgtaaac tcgcgcagga
    13081 aaactttggc gtcgtcgcgt cgtgcggtgg ccgccgattc gatggccgcg ctttcgatga
    13141 agggcgcgac gtgaatatcc gcgcgccggt tagcgacgag cgcgggcggt tgtgcgacgg
    13201 ggaagaactg gagcacgaaa tccgtctcgg ggatgcgcac catgtcgccg tctttgatgg
    13261 ctgcgccggg cgcgagcggt tcgtcgttgt gcgtgaggcg gagcgagcgg tcgaaatcgc
    13321 cgatgcgata gaggccgttg gtgcgtcgca gttcgagcag gaagccgtcg gcgcgcggga
    13381 tcgctccggg ctggaagcgc aactcgcaat cggcatcgga gccgatgcgg atacgctcgt
    13441 gcagcagcac ttccgtatgc cgctcagccc cgaattctac gtgtacgacc agaccacttg
    13501 ccataaagct gaaacttgat ccgcccccgc tactactatt atcaacagtc gctctcaatt
    13561 aaagaggcga ccgcgacgcg accgcctctt aacattctgc ggtcatcctg cgcgcagtcg
    13621 ccaacgccgc ttattttata ctaagattaa ccatacgggc aaaaaccagc catgatttca
    13681 gaccgggcgt gcgagcgtga aggctctcgt ccgtgcccgc aactcaatat gacggattgg
    13741 cggtcgcgct aactattcgg cgaccgctct cgtttgtgaa tcgagcaatt atgaaagaaa
    13801 tctcttttat cgaagataaa gatcaagtgt gcgcgctggc tcccgtcgcc tatgctccca
    13861 tccgtctgca tgtcctggta tgcagcggaa agtcctgcac gagcgtcggc agcgcggaag
    13921 tgaaagaggc tttcgagcgc gaactcaagg cacgccaact tctcttcggg aaagccgcga
    13981 agggcaagaa cccgcagggc agcatcgtgc tcacggaatg cgcctcggtc ggcttctgcg
    14041 ccgtcggcgc ggcggtgctc gtctacccgg acggcgtgtg gtacgcgcag gtgcgtgccc
    14101 gcgatgtgcc ggagatcatc gaagagcatc tcctcggcgg tcgcgtcgtc gagcggctcg
    14161 cgctgctcaa actgcccgcc gccacagccg aaaccgcctg aagaacattc gcgcgagcag
    14221 gttcgcgtta aaacaaaacc gcgcggcgac tcgtaaacaa cagagccgcg cgcacgcagt
    14281 tttatatcca cgccctgacg cgtgtttgcg tgtgcaacag tctgcgcacg cccctgcatc
    14341 caaaggggca gacgcgtgcg ccgtcagttc agacagccta cagttaattc tggagtcaac
    14401 aatttatgtt ccgtttttct cgaaaagttc tgcgaatttc gttcgccgtg ttggttgcgc
    14461 tgctgcttgt gccgcagacg ccgaaggcgc agacgaaagt gccgcgcctc gattatcgtg
    14521 agcgcacgct tcagaacggt ctgcgcgtct tgagcgcggt ggacaaatcc agcccgaccg
    14581 tggcgattca ggtctggtac aaagtcggct cgaaggacga cccggaaggc cggagcggtt
    14641 tcgcgcacct ctttgaacac atcatgttca agagtacgaa gaacatgaaa tccgaaatga
    14701 tggatcgcct gaccgaagat gtaggcggca tgaacaacgc gagcaccgcc gacgacgtga
    14761 cgatctatta cgaggtcgtg ccgtcgaatt atctggagac gctgctctgg gcggaagccg
    14821 accgcctcgc ctcgctcaac gtggacgatg cgaacttcaa atccgagcgc gacgtggtga
    14881 aagaagagta ccgcgagcgc gtggacgcga accccttcgg caaacttttc tacatactga
    14941 tgcaggagaa atccttcacc cggcatcctt acaaacgccc cggcatcggc aatatcgcag
    15001 aactggacgc cgccgccatc gccgacgtgc gcgccttcca ttcgactttt taccgccccg
    15061 ataacgccac gctcgtcgtc gtcggcgact tcgaccagaa gcagttcgat gcgtgggttg
    15121 ataaatattt cgcgcccatt ccgaagccga atcaacctct gccgcgcgtg accgtcaaag
    15181 aaccggagcg cacgggcgag acgcgcatcg tagagtttgc gccgaacgtg ccgttgcccg
    15241 ccgtcgcctt ctcgttcctc gcgccgccga aggccagcga agattccgac gcgctgcgca
    15301 tcgccgagtc gattctttcg tcgggcgaat cgtcgcgcct ctatcgcgcg ctcgtctacg
    15361 aacaacaggt ggcagcgcag gtcagcgcgg gcatggactt gcgcgaagac gtgagcctct
    15421 tccagttctt cgccatcgtc gcgggcggca agaagccgga cgacgccgaa cgcgcgctcc
    15481 tcgccgagat caaaaagatg caggacgcgc ccgtctccgc tgcggaactt gagaaggcca
    15541 agaaccagat catcaccaat caattgcgcg aacgcgaaac caacaacggc aaggcgctcg
    15601 ccctcggcga cgcggcggtt ctcttaggcg atccgaaccg cgccaacacg gacatcgaac
    15661 gcctctccaa agtgacggcg gcggacgtgc agcgcgtcgt gaaaaagtat ctgaccgaca
    15721 cgaaccgcct cgtgatctat tacctgcccg aatcgctccg ccccgcgcaa cctgcggcgg
    15781 agaagaccgg acagacgagc gcgccgcgcg acgcacgcga agccggggga ggaaaataac
    15841 gtgagcacca gcaaagaact ttcgcgtcgc accttagaac ttcgtgcctt gaaacttggc
    15901 accttaaaac ttctcgcgct cgccgccttg tttgcctcgt cggctttcaa tgctgcggcg
    15961 caacaggctg cgccacaggc agcggcacag gcgacgcccc cgccgcccgc cgcgccgcgc
    16021 agcgtcaccg tgccgaaacc cgtcgagcga actctgccga acggcctgcg cgtcgtcgtc
    16081 gtcgagcgca gcaacatgcc gctcgtctcc gccgcgttcg tgatcaagaa cggcggcgaa
    16141 gttgacccgc cgcaactggc cggagtcgca gacctgaccg cctcgctctt aacgaagggg
    16201 acggccacgc gcaccgcgcc gcagatcgcc gaagccatcg aagcgctcgg cggctccatc
    16261 gaatcgggtg cgcgttggga cgcttcgacg gcgagcgtcg gcgtgatgtc ttcgcgcatt
    16321 aacccggcga tggagatact cgcggatgtc gtgcgtcgcc cgacgttcaa agatgaggag
    16381 atcgaacgcc tgcgccagca gtatctagat gatctcaccg tcgcgctcgg ccagcccggt
    16441 tcaatcgcgc gttacgtcgc ctcgcgcgtc gtcttcggcg atgcgcctta cggtcacccg
    16501 ctgtcgggca cgcccgaatc aatcgcgcgc atcaaacgcg acgacatcgt gcggatgcac
    16561 gcgacctact atcgtcccga caacgccatc ctcgttatcg gcggcgacat acgccccgaa
    16621 gaagccttca agaccgccga acgctttttc ggcgactggc agaaacccgc cgcgtcgctg
    16681 cccgctccgg gcgcgaaagc tacggcggcg gccacgacgg gcgcaagcgc gcagccgcgc
    16741 atcgtcgtca tagacaaacc cgatgcgggg caggcggcgg tgctcgtcgc acgcacggga
    16801 attcgtcgca ccgacccgga gttctttcgc ggcatcgtcg ccaactcggt cttgagcggt
    16861 tattcggggc ggctcaatca ggagatacgc atcaagcgag ggctgagcta cggggcacgc
    16921 agcgcgcttg agacgcggcg cgacacggga cctttcatgg cagaggcgca gacgaagaac
    16981 gaatcgggcg cggtcgtggc aggactcctg ctcggcgaac tcagccgtct cgccaacggc
    17041 gaactcccgg aagcggaact gacgccgcgc aaggccgtgc ttatcggcgg cttcgcgcgc
    17101 aatctggaaa ccgccgacgg actggtggcg caggtcgcaa acctcgcgct ctacggcctg
    17161 aacttcgagg agatcaataa ctacatcaac aacgtgcaga cgatcacggc caaagacgtg
    17221 cagggcttcg ccggttcgcg gctcgactcg cgtacggcca acgtcatcat cgtcggcgac
    17281 gcgaggaagt ttctccccga actgcaaaag cagtacaaaa acgtcgaagt gattcccgtc
    17341 gccgaactcg acctcaacat cgcgcaactg cgcaaggcga aatagcagag aaaaacgatg
    17401 aacaacgagc gatgaaggat gaaggatgaa agagagcctt cgatcagttg aatggttctc
    17461 ttattcatcc ttcatcgttc cgccttcatc cttcgatctt actactctcc tcaaacaacg
    17521 cccgcgtctc tcccgtctca gccgaagcca gagaggaatt tgtcatgcca tctaaattaa
    17581 ctctattaac tttgaagccg ctcgcgcttt taagttgcgc gctcatcgtc ctgttcacct
    17641 tcgtcgccga cgcgcgggtg ttgcagccgc agcaagacga cgcggtcaac gagaaaccgg
    17701 caaacgtgag catcctgatg cggcggttga cggatcgaga tgcgttcgtg cgccagcgtg
    17761 cggcggaaga gttggcgcgc cttgcagaca cgcggcagcg caaactcgtc gaagggtatc
    17821 gcctgcaaga gaagcacccg cgcgtgcgcc tcgcgctcga ttgggcgctc taccggatgg
    17881 gcaagcccga cacgctctat acggttgtgc gcgatctcga ttccacacgt tacaaccaat
    17941 ccgaagctta tctggccgaa ctcgaaacgc ccgaaccgct ctacgctatt ctcccgcgcg
    18001 tcaacggcaa cacgcagatc aaactgctcg aagcactcgc gcgcgccggt aacgccgaca
    18061 ctttggagat cgtccgtccc tacacggagt ctcccgaccc gaagatcgcc gacgccgcgc
    18121 gcttcgccac acgcgagatc acgcgccgcc tcgactcccc gcaaccgccc gacgcgaaaa
    18181 cgcgcacgcg ccaaaccggc aacgtgcccg ccgacattcc atagttcaac gtttagaagt
    18241 tccaaccgct ccgcgtcctc ctttgcgcct ccgtcctgaa atgttttcac gacggaggcg
    18301 caagggaagg gcttcgggtt ttgctcgcgg cagggaaggt gcgagtgtgt ctgctcgcac
    18361 cttctcgctt tgtatggtat taaaagctga ttgctaaacg ctaactgcta accgcttcta
    18421 tgcaccgaaa ccttcgttcc ttcctcgatc aactcacacg cgagcgcgat ctcgtcgtcg
    18481 tcgaaacaga ggttgacccg tatctcgaac tggcggaaat tcatcggcgc gtcatcgcgc
    18541 agggcggctc cgcccttctc ttcaaacgtg tgaagggcag ccgttatccg gtcgtcacga
    18601 acctgttcgg cacggcgcgt cgcatcgaac tcgcgttcgg caagaagccg gagtcgctcg
    18661 tcagagaggt tgtccacgtg gtcgagtcaa tgctgccgcc gcgcccgtcc gaactgtggc
    18721 agcatcgcgc gctcgcgctc gaagccttca aactcggaac gcgacgcgtg cgtgaggctc
    18781 ccgtgctgga agtcgtggat cgtccggcgc ggctcgatga gttgccgatc ctgacgacgt
    18841 ggcaggagga cggcggagcg ttcgtgacgc tgccgctcgt ctatacggaa cacccgcgca
    18901 cgcgcaagca caaccttggc atgtatcgca tccaacgcta cgacgccgcg acgacgggca
    18961 tccactggca gatacacaaa ggcggtggct ttcattatca cgaagcggag gccgcgaacg
    19021 agtctctgcc cgtcacgatc ttcctgggcg gcgcgcccgc actgatgttg tcggccatcg
    19081 cgcccctgcc cgaagacgtg cccgaactcg tgctcgcttc cgtcctcgcg ggcgaaaaga
    19141 tcagggtgac gagagacttg ttcacgcacg gccaccggct cgtggccgaa gcggagtttg
    19201 ccatcgccgg gcgcgtgccg cccaaagaac gacgccccga aggacccttc ggcgatcact
    19261 acggttacta ttctttgcag cacgactatc cggtctttca cgcggaggcc gtctgccatc
    19321 gccgcgacgc gatctacccg gcgaccgtcg taggcaaacc gcgacaggaa gatttcttca
    19381 tcggcgatta tttgcaggaa ctcctgtcgc cgctctttcc gctcgtcatg ccgagcgtgc
    19441 gcgacctctg gtcttacggc gagacgggtt ttcattcgct cgcggcggcg gtggtgcgcg
    19501 aacgctacgc gcgggaagct ctagtctcag gctttcgcat actcggcgaa ggccagcttt
    19561 cgctcaccaa gtttctgttg ctcacagaca cgccgcaaga cctgcgcgac ttcccgcgtc
    19621 tcttcgaaca catcctcgcc cgcgcgcgtt tcgagagcga cctgtttatc ttctccaacg
    19681 tctcgatgga cacgctcgat tacacgagcg gcaaagtgaa tcagggcagc aaagcgatgc
    19741 tgctcggctt gggcgaggcc gtgcgcgaac tgccgcgaga gtttcgcggg caacttccgg
    19801 cgaaggtgac gcgcgcggaa gtcttctgcg gcggctgtct tgtcgtcgcg ggcgagcctt
    19861 acgcgtccga cccgcagcag gccgcgcgcc tcgcgacgga agaagcgttt gccgattggc
    19921 cgctcgtcgt cctgcacgac gacgcgagcg tcgcgcgctc gtccgcagat ttcctctggg
    19981 cgacgtggac gcgcttcgaa cccgcctccg atatttatgc ggcacggacg agcgttgagc
    20041 gtcatcatct ttcctaccgc ccgcccgtcg tcatagatgc gcgcatgaag ccgaattatc
    20101 ctgacgaact cgtcgtgcgt cctgatatcg ccgccctcgt agaccgtcgc tggcgcgaat
    20161 atttcccgtc gagcgacctt gtcaaagata gttgagttgt tcgcctcgtc ctcgcaggga
    20221 gaaacagatg agcgagaacc aaagcgtcga gacgtttgaa acctcttgtt gcattgtcgg
    20281 cggtggtccg gcgggcgcgc tgctcgcgct catcctcgcg cggcaaaagc tccccgtcgt
    20341 cctgctcgaa gcgcacgaag atttcgaacg cgattttcgc ggcgacacgc tccacccgtc
    20401 cgtcctcgaa atactcgacg aactcggcct cgccgaacga ctccacacct tccgccattc
    20461 caaaattcac accgccactt tcatgacgcc ctccggcgcg ctgacggctg ccgatttccg
    20521 acgcctcaac acgcgcttcc cctacatcat gctgctgcca caagcacact tcctcgaatt
    20581 catcaccggg gaggctaagc agtatccgaa ctttcaactc aggatgggag cgcgcgtcga
    20641 agagttgatc gaagaggcgg gcggagtgcg cg
//
LOCUS       DASWNS010000626         5986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_205996, whole genome shotgun sequence.
ACCESSION   DASWNS010000626 DASWNS010000000
VERSION     DASWNS010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205996"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..494)
                     /locus_tag="VGB76_21780"
     CDS             complement(27..494)
                     /locus_tag="VGB76_21780"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEY0101572.1"
                     /translation="MFKPRARERDLIVEELKDETLVYDLESHRAHCLNRSAALVWKQC
                     DGRRSVAEIAGNLSREFPAGADDEMVSFALEQLAKFKLLEERGGKPAGAKTGLTRREM
                     MRRLGLAAAVGIPLVTSIVAPTPAQASTCIPSDGACTSSAQCCSGVCAGETCA"
     gene            complement(746..1516)
                     /locus_tag="VGB76_21785"
     CDS             complement(746..1516)
                     /locus_tag="VGB76_21785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101573.1"
                     /translation="MRVGVRANHADALAQLLPHLPPGWKASRNSAVERLFSLIVGGAS
                     RAERGVSRFNLLYEDHVRMARARDLGEVLDIMESRLRLFVAERARRRIFIHAGVVGWR
                     GRAILVPGRSMSGKSTLIAEFVRAGATYYSDEFAVLDERGRVHPFAKPLSLRREGETR
                     QENFGVEELGGRAGTKPLPVGLVLSTEYALGARWRPKVLSPGQGALALLRHVVAARTE
                     PAMALAYLERVVASAPVLMGRRGEALDFARKILAESLF"
     gene            complement(1860..2528)
                     /locus_tag="VGB76_21790"
     CDS             complement(1860..2528)
                     /locus_tag="VGB76_21790"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0101574.1"
                     /translation="MKPAQNGITPLMLAASRGDLATFEDHLAQAGEDVNAQDNFGYTA
                     LMYAASAGHTRVVEALLAAGSDLQKRNRQGLSSLDLALAKGHAGVVLQLRQSRLFFAA
                     RDGDLSELTELLEQGVDVNAQVTDGWTALMIAAFHNHPHVVSTLLLRGADPELETITG
                     RTALMIAEHKGHGEVAGLLHHYGAAPLASSPSAGTNTTIAPTPPTAEHEPAPAPGIPT
                     GPDN"
     gene            complement(2676..2861)
                     /locus_tag="VGB76_21795"
     CDS             complement(2676..2861)
                     /locus_tag="VGB76_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101575.1"
                     /translation="MAARQGRGERVDEQRDEEERHQRKERGQQVPERKSAAKIETPFE
                     RGARVGGVVCGEGVAAL"
     gene            3416..5155
                     /locus_tag="VGB76_21800"
     CDS             3416..5155
                     /locus_tag="VGB76_21800"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0101576.1"
                     /translation="MKVIPLHSAPSGGRCSLLLLLIGLFCFNTTSVSAQQEATSPARA
                     SSVVRGRIVYEDTGKPMRYARVFLSTEKSRQRERLTSTDARGEFIFRNVAAGNYLASV
                     LPPDVFGSNSYPSAPEGGETVSVDGTSAVELKLRGARGGSISGKVTYADGEPVVSARI
                     VLLRAVGEHWNAVGQCCTGGPVTDDRGVYRLYGLPPGEYKVGVAEEGLQVESSEGGGI
                     SSGSNQSLLTFFYPSVVGQEMATLIKVSPGRAVEGIDVALPEVRTFNVSGTVSLGGQL
                     MRGVTLTLQMKNSQGHKIATTDADGKWVFKSVPDGTYSISTQPGSVSWNSDPEITAKL
                     MKAVQTSRSVTVSGADVEGVTIALSEGAAIMGTVVVEGGVALPQHMELMTTRTDDALR
                     PSGEGMGRGKVEPGGSFRIEGVKPGEVFIDLGIYPSGYHYVKSIMWNGQDLLREPLAV
                     TGGGEIKRVRVTLATGLATLKGSVTATVAAAGQQLQQAAEPCVVVLVPADPALLRRRS
                     FYLWAELSPENKFSMKGAPGEYLAMPLNPKFGNNLAQYIKDHAQGAQRVTLRAKEETK
                     LELSRGCERETAK"
     gene            complement(5228..>5986)
                     /locus_tag="VGB76_21805"
     CDS             complement(5228..>5986)
                     /locus_tag="VGB76_21805"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEY0101577.1"
                     /translation="GKWYIYFAADDGRNANHRLWVIENASPDPLRGEWVMRGKLADPS
                     DKWAIDGSVFEHRGQLYLVWSGWEGDTNGRQDIYIARMKNPWTLEGARVMISAPEHPW
                     EKVGDLGEDRDPEDPPHVDVNEGPQILKRADKLFLIYSASGCWTNYYSLGMLTAAADS
                     NLLDRRSWTKSPRPVFQQSPVAGAYGAGHNSFFKSPDGREDWILYHANPKSKQGCGGH
                     RSPRAQPFNWKPDGTPDFGQPVPIDKAIRKPSGQ"
BASE COUNT         1106 a         1813 c         1895 g         1172 t
ORIGIN      
        1 gcactcgtga aagtatactg acgaactcat gcacaagttt ctccagcaca gacgcctgaa
       61 caacattgag ccgatgaggt gcaagccccg tcgctcggta tgcaggtgga ggcttgcgcc
      121 ggagtgggcg cgacgataga agtcacgagg gggatgccga ctgctgccgc cagccctaac
      181 cggcgcatca tttcgcggcg cgtcaatccg gtcttcgcgc ccgccggttt ccccccgcgc
      241 tcttcgagca gtttgaactt tgccaactgt tccagcgcaa aactgaccat ctcgtcgtct
      301 gcgcccgcag gaaattcgcg gctcaagttt cccgcgatct cggcgacgct gcgccgcccg
      361 tcgcactgct tccagacgag agcggcgcta cggttcaggc agtgtgcgcg gtgagattcg
      421 aggtcgtaca cgagcgtctc gtccttcagc tcttcgacga tcaggtcgcg ctcgcgcgct
      481 cgcggcttaa acatgctctc ttctttcatc gagcctccga ataaattgat ggcgggtaga
      541 gtctcctacg actcgccgtg atgacagtcc tcccgccccg tcactgatct gtgtaaggct
      601 tgggcgggtg aaccacgggc tactatttcg aacttgcaaa gttttaccgg cggtgagaat
      661 ccctaaggga ctgagcacgc gcgaataact gagcacgcgt gaatagatga ggcgacttgc
      721 gcaatatagc acaacttgtc tatggctaga acagcgattc cgccaaaatt tttcgagcga
      781 aatcaagggc ttcgccacgc cgccccatca agacgggcgc gcttgcaaca acccgctcca
      841 gataggctaa ggccatcgcc ggttcggtac gcgcggcgac gacgtggcgt aggagcgcga
      901 gcgcaccctg ccccggcgac agcaccttcg gcctccagcg tgcccctaaa gcgtactccg
      961 tggacagcac caggccgacg ggaagcggct ttgtgcccgc gcgcccgccg agttcttcga
     1021 ctccgaagtt ctcttgccgc gtctctccct cccgccgcaa agagaggggc ttggcgaaag
     1081 ggtggacgcg ccccctctcg tcgagcacgg cgaactcgtc cgaatagtag gtcgccccgg
     1141 cgcggacgaa ctccgcgatg agcgtggact tccccgacat gcttctgccc ggaaccagaa
     1201 tggcgcggcc acgccagccc accaccccgg cgtggatgaa tatgcgccgc cgcgcgcgtt
     1261 cggcgacgaa gagccgtaga cgcgactcca tgatgtcgag cacctcgccc aagtcgcgcg
     1321 cccgcgccat ccgcacgtga tcctcgtaga gcaggttgaa gcgactgacg ccgcgctccg
     1381 cacgcgatgc gcccccaacg atcaaggaga acaggcgctc cacggcagag ttacgcgaag
     1441 ccttccagcc gggcggaaga tgtggcaaga gctgcgcgag cgcgtcagcg tgattggcgc
     1501 gtacgccgac gcgcacgccg tagagtgtga aggcgagtcc tgccgcccag cccaggcggt
     1561 cgagtttctc gattcaagca gtacgcgtgc gaaggcgcgg cattgaaggc accgctgccg
     1621 tcgtcggctc gtttcgttta tgaatggggt cttcagacat tgccagtttg acggacgggt
     1681 ttaagaagag cacagcttga cttgagccgg gagtttttcg ccgccggagt tttattgacg
     1741 acgcaccgca tcctgctctt ttgtagtgca aatttttcgc agctatgccc accgaggagg
     1801 catcgtggca tgcgagccag tcccggtcaa gcgaggcttc ttttatcgcg ggagaagaat
     1861 cagttgtccg gcccggtcgg gatgccgggt gccggagcag gctcatgctc cgccgtcgga
     1921 ggcgtcggag caatggtcgt attcgtcccc gccgacggag acgacgcgag aggggctgcg
     1981 ccgtaatggt ggaggaggcc ggcgacttcg ccgtgccctt tgtgttcggc gatcattaaa
     2041 gccgtccgcc cggtgatggt ttcgagttcg gggtcggctc cgcgcaggag gagtgtggag
     2101 acgacgtgcg ggtggttgtg gaaggctgcg atcatgagcg ccgtccatcc gtccgtgacc
     2161 tgcgcgttca cgtccacgcc ctgctccaaa agttctgtga gttcggagag gtcgccgtcg
     2221 cgcgcggcga agaagaggcg cgattgccgc agctgtagca ccacccctgc gtgccctttg
     2281 gctaaagcca ggtccagact cgacagccct tgccggtttc tcttttgcag atcgctcccg
     2341 gcggcgagca acgcctcgac gacgcgcgtg tgtcccgcgc tcgccgcgta catcagcgcg
     2401 gtgtagccga agttgtcctg cgcgttcacg tcttcgcccg cttgagcgag gtgatcctcg
     2461 aacgtggcga ggtcgccgcg cgaggcggcg agcatcagtg gggttatgcc gttttgcgct
     2521 ggcttcatcg gatactaacg cccggtcgga gatcaaagct gtcacgagag aagactaagt
     2581 agaacagttc gggaggaggt gtcaaggaat tcgcaagcgt tgaacgggcg cggcatcttc
     2641 tttatgcggc gcttcattcc ctcctctgat gcccgctaga gagccgcaac gccctcgccg
     2701 cacacgacgc cgccaacgcg tgcgccgcgc tcgaaaggag tttcaatctt tgcagcagac
     2761 ttccgctccg gtacctgctg gccgcgctct tttcgctggt gacgctcttc ctcatcgcgt
     2821 tgctcatcga cgcgctctcc gcgcccttgt cgcgccgcca cgcaatgctc tacagtgagg
     2881 catcactcca gcactcaggt caggagagaa aggaacctgt ccttgctttc tctcttcccc
     2941 atttcgcaat ttcggccagt atcgtttatg atggtcaaca tctggcatgc ggtgaatcca
     3001 aaatgccaag tggttgctgg ggtgaggaca gtaaccacat atagaacagg cgcggtggcc
     3061 cccaaggccg ctgcgcctgt tcgcttattt ggcggacgac tccaaacgtt ccggagcggc
     3121 ttccgcttct taagtcgacg ccaatcccaa agcacttatt tgcgctgtgc caagttgaac
     3181 tgcccggaac ggccagaggt tgttcgggtt ctcgcggctc tgcgggcgca agccggcgcg
     3241 acgcggcggg gtttgcggcg tggcattaac tttcgcgcct cctgtctacg gaaagagttt
     3301 gcatcttgac tctgccccgg taagcgttac aatccacaca gcctcaggcc gcaacagtcc
     3361 tgccttttgc ggcacagtca aatcacacga tacttcttcc acaggagtgt tccagatgaa
     3421 agttattcct ctccattccg caccctccgg cggtcgttgt tctttattgc tcctcttgat
     3481 cgggttgttt tgcttcaaca ctacaagcgt ttcggcgcaa caggaggcga ccagtccagc
     3541 ccgcgcctca tcggtcgtac gtggtcgcat tgtctacgag gacacgggca agccgatgcg
     3601 ttacgcgcgc gtctttctct ccaccgagaa gagccgccaa agggagcgcc tcacctccac
     3661 ggacgcgcgt ggagagttca tcttccgcaa cgtcgccgcc ggaaattatc tcgcgtcggt
     3721 cttgccgccc gacgtgttcg gctcgaacag ctacccctcc gccccggaag gcggcgaaac
     3781 cgtgagcgtg gacggcacct ctgccgtcga actcaagctg cgcggagcgc gcggcgggtc
     3841 aatcagcggc aaggtgactt acgcggacgg cgagccggtc gtgtcggcgc gcattgtgct
     3901 cttgcgcgcc gtgggtgagc actggaacgc ggtcgggcag tgctgcacgg gcggccctgt
     3961 gacggacgac cgcggcgtct atcgcctcta cggcctcccg cccggcgagt acaaggtggg
     4021 cgtggctgaa gaaggtctgc aagttgaaag cagcgagggc ggcggaattt cctccggttc
     4081 caaccaatca ctgctgacct ttttttaccc ctcggtggtg gggcaggaga tggcgacatt
     4141 aatcaaggtt tcacctgggc gggcggtgga gggcatagat gtcgccttgc cggaggtgcg
     4201 cacgttcaac gtctccggca ccgtctcgct cggcgggcag ttgatgcggg gcgtgaccct
     4261 cacgctacag atgaagaatt cgcaaggcca taaaatagcg accactgatg ccgatggaaa
     4321 gtgggtgttc aagtctgtac cggacggcac ttacagcatt tccacgcaac cgggcagcgt
     4381 gtcgtggaac agcgacccgg agataacggc caaactaatg aaagccgtgc agacgtcgcg
     4441 tagtgtgacc gtctccggcg cagatgtcga aggggtgacg attgctctgt cggaaggcgc
     4501 ggcgatcatg gggacggtgg tggtcgaggg gggcgtcgcg ttgccgcaac acatggagtt
     4561 gatgacgacc cggactgacg atgcgttgcg cccatcgggc gaaggcatgg ggcgcggaaa
     4621 ggtcgagccg ggcggctcgt tccggatcga aggagtgaag ccgggcgagg tcttcatcga
     4681 cctcggaatc tacccttcag gctaccacta cgtgaagtcg attatgtgga acgggcagga
     4741 tttgctcaga gaaccgctgg cggtgacagg ggggggcgag atcaaaagag tgcgcgtcac
     4801 gttggcgacc ggcctggcga cgctcaaggg gagcgtgacc gcaacagtcg ccgccgctgg
     4861 acaacaacta cagcaggccg ctgagccgtg tgtggtggtg ctggtgcccg cagaccccgc
     4921 gctgttaagg agacgctcgt tctacctctg ggcagagttg agtcccgaaa acaagttcag
     4981 catgaagggt gcgccgggcg agtacctggc gatgcctctg aacccgaagt tcggtaacaa
     5041 cctggctcag tacataaagg atcatgcgca gggcgcgcag cgtgtgacgc tcagggcgaa
     5101 agaagagacg aagctggagt tgagccgtgg gtgtgagcgg gagactgcga agtgatcgtc
     5161 cccgtcgtgc tcaaggcggt cggggagttg gcctccggcc tcgggcgcgg ccttgcgaat
     5221 accagcatca ctggccggaa ggtttccgaa tcgctttatc tatgggcacg ggttggccga
     5281 agtcgggcgt gccgtcgggc ttccagttga acggttgcgc tctcggcgag cgatggccgc
     5341 cgcagccctg tttcgacttc gggttggcat gatacagaat ccaatcctcc cgcccgtcgg
     5401 gcgacttgaa gaaagagttg tgccccgccc cgtaggctcc cgccacgggc gattgttgga
     5461 agacggggcg cggtgatttc gtccacgatc tacggtcgag caggttactg tcggcggcgg
     5521 cggtcagcat gccgagcgaa taatagttgg tccaacaacc gctggcggaa tagatcagga
     5581 acagtttgtc tgcgcgcttc aaaatttgcg gaccctcgtt gacatcgacg tgcggggggt
     5641 cttccgggtc gcgatcctcg cccaagtctc cgaccttctc ccaggggtgt tcgggtgcgg
     5701 agatcatcac gcgcgcacct tcgagcgtcc aggggttttt catccgtgcg atgtagatgt
     5761 cctgacgacc gttcgtgtcg ccctcccatc cagaccagac gaggtagagt tgccctcgat
     5821 gctcgaagac cgagccgtca atcgcccact tgtccgaagg gtctgcgagt ttgccccgca
     5881 tcacccactc gccccgcagc ggatcgggcg aagcgttctc gatcacccac aggcgatggt
     5941 tggcgttgcg cccgtcgtcg gcggcgaagt agatgtacca cttgcc
//
LOCUS       DASWNS010000627         3711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_127986, whole genome shotgun sequence.
ACCESSION   DASWNS010000627 DASWNS010000000
VERSION     DASWNS010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127986"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1212)
                     /locus_tag="VGB76_21810"
     CDS             complement(244..1212)
                     /locus_tag="VGB76_21810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101578.1"
                     /translation="MKIQRFKFIAAFAVLLLLLSGATDARAQLGGLGGLGDKLKKKTP
                     GVSSLLEGKPPITTSLPDAKWADASKDNFTPREAKRSLMTLQRTPNGGFVVQPGYYEM
                     QTQSYCLKAGTHGPGGGDGYLYAPPLGPAEDAVMTIVRNSVNRPEIPQQKIQQLLWAI
                     IARAKFEDLQGEIKAVASQLLTPRQLASLNRSALDVLSNSQLQGALGGVPAPLRQVLE
                     AEAQLRQMLTTPGASFAEMERVAVLSGAAPRGEGSRDVPGGRWSLHPDGYYVRYIPGG
                     YSSTRVEIWVPQGSPAVGKEYDPATHVAVPGNTARQRLIQSGRAHQ"
     gene            1522..2019
                     /locus_tag="VGB76_21815"
     CDS             1522..2019
                     /locus_tag="VGB76_21815"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY0101579.1"
                     /translation="MNRLFDVDWNLLFIPDTPVLEIILRGTILYIIIFFFVRVLPNRQ
                     IGGVGMNDLLLIVLIASAATNGLAGDYKSITSGVVLIATIIFWSYLFNWVGHRFSLFH
                     RMFHPEPTPLVKDGEIQRDNMRRELVTEEELIGKLRRQGITDVKGVSEAHVESDGQIS
                     VIERK"
     gene            complement(2235..3137)
                     /locus_tag="VGB76_21820"
     CDS             complement(2235..3137)
                     /locus_tag="VGB76_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101580.1"
                     /translation="MSNFIDVGGYGSQTTEIYLKRVLAGNIESVRARLGVALERLGYD
                     LLEEEPALHGRRGARGWGVWYSSADVLDYPMTLIVRLKSAGEHSTHATFDYTINHPWL
                     SKGEKEVLTREAEAIVAIASTRAAEKVCAGCATEATDDSRFCRRCGAPMTSEQAELDV
                     LRMAAETRAGHTSVVTSTILSLSSIAIALLALVVYLKKGAVTTKGLWLLLSVMAVISL
                     FNFLVGRFAWQRLNAALASRREETRTLPGRRVQTLPNIEPAALPPRHIGHSVTEGTTE
                     LLNAQTREQEPIHLRRGGDTSSVS"
     gene            complement(3258..>3711)
                     /locus_tag="VGB76_21825"
     CDS             complement(3258..>3711)
                     /locus_tag="VGB76_21825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY0101581.1"
                     /translation="GNSSGITDGAAAVVVLGEEALRETKGVEPLARIVDYEIAGVAPE
                     VMGIGPVPAIRALLARQNLSLADIDLVELNEAFAAQVIACDRELGFDAARLNVNGGAI
                     ALGHPIGCTGVRITTTLLHEMQKRNARRGLATLCVSGGLGLALLVERV"
BASE COUNT          699 a         1083 c         1188 g          741 t
ORIGIN      
        1 gagcagcgga cggggacgaa gcatagacga atcacggacc acgttcggga atttgagagg
       61 ttgaaggaca tatagcacgc ccttgcatca ggtcggatgc cttaacgtgg attacaacgg
      121 ctgagagaga accgcagttc cgcgcagagg ccgagattta tggcagcaaa atggggtcgc
      181 acgtctatgt gcgaccccat tcgttcaggc tcaattgttt tgacgttgac cggcgcgtgc
      241 ggcttattga tgcgcgcggc ctgactggat caacctctgt ctcgcggtgt tgccggggac
      301 agcgacgtgc gtggccgggt cgtactcctt gccgaccgcc gggctgccct gcggaaccca
      361 gatttccacc cgcgtcgaac tgtacccgcc cggtatatag cgcacgtaat agccgtccgg
      421 gtgcagcgac cagcgaccgc ccggcacgtc gcggctgccc tcgccgcgcg gagccgcccc
      481 tgagagcacg gcgacgcgct ccatctccgc aaacgatgcg cccggcgtcg tgagcatctg
      541 ccggagttgc gcctcggctt ccaagacctg acggaggggc gcgggaacgc cgccgagcgc
      601 accctgcaat tgcgagttgg agagaacgtc gagcgcgctg cggttgagcg aggcgagttg
      661 gcgcggcgtc aacagttgcg aggcgacggc cttgatttcg ccctgcaagt cctcaaactt
      721 cgcgcgggcg atgatcgccc agagaagttg ctggattttt tgctgcggga tttcggggcg
      781 gttgacggag ttgcgcacga tggtcatcac ggcgtcttcg gcagggccga gcgggggcgc
      841 gtagagatag ccatcgccgc cgccggggcc gtgcgtcccg gccttgaggc agtagctctg
      901 cgtctgcatc tcgtagtagc cgggctgcac gacgaagccg ccgttgggcg tgcgttgcag
      961 cgtcatcagc gagcgtttag cttcgcgcgg cgtgaaattg tctttcgacg cgtctgccca
     1021 tttcgcatcc ggcaggctcg tcgtgatcgg cggcttgcct tcgagcagcg aagagacgcc
     1081 cggcgtcttc ttcttcaact tgtccccgag gccgccaagt ccgcccaact gcgcgcgggc
     1141 atccgtcgcg cccgaaagca gcagcaacaa gaccgcaaac gcggcaataa atttaaagcg
     1201 ttgtatcttc actaaaaatt ctccctgaaa attaaggact gaaatttgag gttgatccct
     1261 cgatcctgaa aaaactttag cggcgaagag tcgtttccag ccggcgcgtt tgcaagaagc
     1321 ctagacgccc tatccggcct gatggttgtc ttgatcgggg gattgtgtca acggcagcgt
     1381 cggatgacga aatagccgcc ttaccgccgc ttgacatctt atacggcatt cttgacatct
     1441 catacggcac tattgaaact ttagatcata cactcttcga aagacaggga aattttacga
     1501 gcaaggggca gatgtgagca aatgaatagg ctgttcgacg tagattggaa tcttcttttc
     1561 attcccgata ctccggtact tgagatcatc cttcgcggga caattctcta cattattatc
     1621 ttcttcttcg tacgcgtcct acccaacaga cagatcggcg gggttgggat gaacgacctg
     1681 ctgctcatcg tgctcattgc gagcgcggcg accaacggac tggccggtga ttacaagtcg
     1741 atcactagcg gagtcgtgct catcgctacg attatctttt ggagttatct gtttaactgg
     1801 gtcggccacc gcttctcact tttccacaga atgtttcacc ccgaacccac accgttggtg
     1861 aaagacggcg agatacagcg tgacaacatg cgcagggaac tcgtcacgga ggaagaactg
     1921 attgggaagc tacgacggca ggggatcacg gatgtcaaag gcgtcagcga agcacacgtc
     1981 gaatccgacg gccagataag tgttattgaa cgaaagtgaa aatagacgca cgcggcctgc
     2041 tgctatcgcc tgcttacgcg ggttgaaatt gagcgtcgcg cgtggcgtct cattcgaact
     2101 tacgcgtttt gttttcgatg ggaaacagtt cggttctgtt ttcgaccacg ctccgcgcac
     2161 cgtctccggc aggagggacg gcaagcgcgc ggcgggtggc gcgtgagtca ccggctttag
     2221 gccgtcgtgg ttgctcaact gacggagctt gtgtcgccgc cgcgcctgag gtggatgggc
     2281 tcctgttctc tcgtctgtgc gttgagcaac tcggtcgtcc cttccgtgac ggagtgcccg
     2341 atgtggcgcg gaggcaaggc ggcgggttcg atgttgggca gcgtttgcac gcgcctgccc
     2401 ggcagtgtgc gcgtctcttc tcgccgggag gcgagggcgg cgttcagcct ttgccacgcg
     2461 aagcggccaa ccagaaagtt gaacagcgag atcacggcca tcacggagag caggagccac
     2521 agccctttcg tcgtcaccgc acccttcttg agatagacga cgagggcgag gagtgcgatg
     2581 gcgatggagg agagcgacag gatcgtgctg gtgacgaccg acgtgtgacc ggcgcgcgtt
     2641 tcggcggcca tgcgcagcac gtccagttcg gcctgctcgg aagtcatcgg cgcgccgcag
     2701 cgacgacaga aacgggagtc gtccgtggcc tccgtcgcgc aaccggcgca gaccttttcc
     2761 gccgcgcggg tcgaggcgat ggcgacgatg gcctcggcct ctcgcgtcag cacctctttc
     2821 tcgcctttgg aaagccaggg gtgattgatg gtgtaatcaa aggtggcgtg cgtcgagtgc
     2881 tcgcccgcgc ttttgaggcg gacgatgagc gtcatcgggt agtcgagcac gtccgctgaa
     2941 ctgtaccaga cgccccaccc gcgcgccccg cgccttccgt gcagggcggg ttcttcttcg
     3001 agcaggtcgt aaccgaggcg ttcgagcgcg acgccgaggc gtgcgcgcac gctttcgata
     3061 ttcccggcca gcacgcgctt gagataaatc tccgtggttt gcgagccgta gccgccgaca
     3121 tctatgaagt tcgacatgca gatacctcta ctgcggaatt gcgacgaatg agcagttaag
     3181 attacgtctg agcggggcgt gaggtttcca tttcacccgg acatgcaatt ccgcgccgtc
     3241 gcaaccggac gcgcagccta gacgcgctcg acgagcaggg cgaggccgag gccgccgctg
     3301 acgcacaagg tagcgaggcc gcgacgcgcg tttcgtttct gcatttcgtg gagcagcgtc
     3361 gtggtaatgc gaacccccgt gcagccgatg gggtggccga gcgcgattgc gccgccgttg
     3421 acgtttaagc gcgcggcgtc gaaaccgagt tcgcggtcgc aggcgatgac ctgcgcggcg
     3481 aaggcttcgt tgagttcgac gagatcaatg tccgcgagcg acaggttttg cctggcgagc
     3541 agggcgcgga tggcgggcac ggggccgatg cccatcactt caggcgcgac ccctgcgatc
     3601 tcgtaatcga cgatgcgcgc cagaggctcg acgccctttg tctcccgcaa cgcctcttcg
     3661 ccgaggacga cgacggcagc cgcgccgtcg gtgatgccgg acgagttgcc c
//
LOCUS       DASWNS010000628         8113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_440768, whole genome shotgun sequence.
ACCESSION   DASWNS010000628 DASWNS010000000
VERSION     DASWNS010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440768"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGB76_21830"
     CDS             complement(<1..288)
                     /locus_tag="VGB76_21830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101582.1"
                     /translation="MSGDIFTQRSFVRGEETALSKRPQKRSYRTERDFGLLVGGVLLG
                     LGGWWLYRGKYPTLSVSLVAVGATLMLLGAFLPKSLVVPNRLWMGLAEAMGF"
     gene            complement(474..1418)
                     /locus_tag="VGB76_21835"
     CDS             complement(474..1418)
                     /locus_tag="VGB76_21835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0101583.1"
                     /translation="MNKGRFTLSILTSIIFIFALASLTQAQATRTWVSGVGDDVNPCS
                     RTAPCKTYAGAISKTAKDGEISTLDPGGFGTLTITKSITVNGGGGGQGYGSVLSALAP
                     QGFLVNITDAADIRKTVRLNWLDINGASSGTDGIRMIAGLALHIENTNIDGLTGDGVD
                     INIAGANVTELYMKNVSIRNCVGNGVILNASNASGLIAATLDGVMMNNCGDGFEAALR
                     SLASLRNCVFSTNNGASSAGVRVNGATSEVSVDNCVIAYNTSGVRVDSGTARVAGSLL
                     NGNSTGLQNVGGTLKTFGTNRNDATTPTAGVITPIAES"
     gene            1867..2892
                     /gene="rfbB"
                     /locus_tag="VGB76_21840"
     CDS             1867..2892
                     /gene="rfbB"
                     /locus_tag="VGB76_21840"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY0101584.1"
                     /translation="MQKFFVTGGAGFIGSAFIRQALARWPDAEIVNFDALTYAGNPDN
                     LSGLDETRHRFVRGDITDREAVRAALTEGTDAIFNFAAESHVDRSIERADDFLRTNIL
                     GTQVLLDAAREGGVRRFVHISTDEVMGSLPEDESAFFTEESGYAPNSPYAASKAAAEH
                     IVRAARHTFGLDTVTTRCGNNYGPRQFPEKLIPLLLSNALNDEPIPVYGDGLNVRDWI
                     YVEDHCRAIMLAYERGEAGGIYNIGARNEQRNIEVVESILAALGKPASLIRYVKDRPG
                     HDRRYAIDPAKAERELDWRPVETWESGLAKTIRWYEENTVWIARVRNGAYRDFYARQY
                     GVEVGAK"
     gene            2889..3767
                     /gene="rfbD"
                     /locus_tag="VGB76_21845"
     CDS             2889..3767
                     /gene="rfbD"
                     /locus_tag="VGB76_21845"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY0101585.1"
                     /translation="MKVLITGAGGMVARALAAHCASRGDTVFAHTRETLDITNAGNVR
                     EVFARDQPETVINCAAWTDVDGCELNPERAVLVNAEAVEILATHSRMAGASFVTISTD
                     YVFDGAKEGFYTQRDDPAPQSAYGIAKLQGERRALRASARTVVVRSGWIFGTGGRNFL
                     ATAVERARRGEHLKAISDAYGTPTYAPHLAARLRELAERDLPGTYHVVNSGAGASFFE
                     FAQEAVRAAGVEGVEVESVASDTLARPAPRPRNSRLRCLLSEAIGLAPLPEWREALRE
                     FAGAGKTEGEEGKGKG"
     gene            3947..6364
                     /locus_tag="VGB76_21850"
     CDS             3947..6364
                     /locus_tag="VGB76_21850"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEY0101586.1"
                     /translation="MSQDNRLLPLTPGTNLDRPLRELTQPERPNNTYALAEPTHLRDY
                     LQVVLKRKWLILSLIVVVTSLVAIQMYRMPTIYRSESIIQIEQKKTNLLKTKEFVLNA
                     QTDPAYWGTQLKLLENPRLHRKVILALDLQNNPSFVGGQARPSLFATVRRIFSREKPA
                     ANAPAAAPVGGLDVVNDAPAATTTVETENLSPQQATKLLAYEDIMRAGLTITPVERTN
                     LVKIVYEHTNPELAMKISDTLAAVFIQDNRERETFGSEKSAESLAKNITDLQIAYRQK
                     QEEIIAFKRSHNLPLGAADGQDLTKAILGMSTTQMMQAEDKRKELEASYRVAQNTQDI
                     YSIPEVMANKSVQELRDKLSTLEEQRQALLVQYTEEWPAVKKIDKQKAQVQASLDKQA
                     RDVVRTIEGSYKAAVMRENSLRQSNSTARGQANAQSQSEGHLDIKLQELETIKQLYNT
                     ALQGKKEFDVSGNETPNNITVSTPARRAEIVGPPRVRNILLGLLLSLAAGIGLAFLLD
                     YLDDTLKSIEDVDRHIHLPTLALIPAPRAERRFMPARAALASPPGGEAPPTALALIEE
                     KRSPVAEAYRHLRTSLLLSSAGQPPKTVLVTSSQPSEGKTTTAVNTAVMLAQTGAEVL
                     ILDCDLRRPRIHAHFGLQNTRGVTNYLSGEANISSLIQEYDKLPNLKIITSGPVPPNS
                     AELLGSEEMRRLLVLLSDNFTHIIIDSPPAISFTDASILSTMVDGVMLVVHGGRSSRA
                     VVRRAKQQLLDVGAHIYGIVLNNVKLESSDYYYYSGYYSSYYANEDEDQTVATEADDM
                     VETAKSK"
     gene            6462..6932
                     /locus_tag="VGB76_21855"
     CDS             6462..6932
                     /locus_tag="VGB76_21855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0101587.1"
                     /translation="MREFCAIADDVVLGRDVQIYNFVNLYGCEIGDETRIGTFVEIQK
                     GAHVGARCKISSHTFICEGVRVEDEVFIGHGVQFINDRYPRATRADGTPQTEADWTLE
                     RTIVRRGASIGTGATILGGIEIGARATVGAGAVVTRDVPAGATVAGNPARILKQ"
     gene            7093..>8113
                     /locus_tag="VGB76_21860"
     CDS             7093..>8113
                     /locus_tag="VGB76_21860"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0101588.1"
                     /translation="MIPFVDLQAQYRAIKLEVNEAIARVVESAAFVLGREVEAFESAF
                     AGYLGARFCVGVSNGTAAIQLALMACGIGAGDEVIVPANTFFATAEAVSTAGATPVFV
                     DADPISYTIDVNKIEAAITERTRAIIPVHLYGQAADLDAIFEIAAQRDLAVIEDAAQA
                     HGALYKGSRVGARGRANCFSFYPGKNLGAYGEGGAVVTNDAEVARRVRLLRDHGSEQK
                     YRHEIIGYNFRLEGIQGAVLNVKLKYLDGWNDLRRAHAARYRELLAEAHPGGSDDDSG
                     TLTLPQESPYARHIYHLYVVQSVERDALQKHLSAAGIQTGIHYPVPVHLQPAYASLGH
                     KPGDFP"
BASE COUNT         1654 a         2562 c         2422 g         1475 t
ORIGIN      
        1 gaagcccata gcctccgcca agcccatcca gaggcgattc ggcacgacga gcgacttcgg
       61 cagaaacgcg ccgagcagca tgagcgtcgc gcccacggcg acgagcgaaa cggagagcgt
      121 cgggtacttt ccgcgataaa gccaccagcc gccgagtcct aaaaggacgc cgcccaccag
      181 caacccgaag tcgcgctcgg tgcgataaga acgcttctgt ggccgcttgg acagggctgt
      241 ttcctcacct cgaacaaaac ttctctgcgt gaaaatgtcg ccggacattc aaggaagcca
      301 atgtaaccat gcgctctttc gagcgtcaag caagcatctc acgccccttc cgcgaaggga
      361 cgcgggcaga aagtccgcgc aagttaaccg ggcgcgcgaa gcattaaaaa gttccgcccg
      421 tctcttcgtg aagagacggg cggaactttt cgcatttaag tcgagagtag cttttagctc
      481 tcggcgatgg gggtgatgac gcccgccgtc ggggtcgtcg cgtcgttgcg gttggtgccg
      541 aacgtcttga gcgtcccgcc gacgttttgg agtccggtcg agttgccgtt caacaaactg
      601 cctgcgacgc gcgccgtacc ggaatcaacc cggacgccgc tcgtgttata ggcgatgacg
      661 caattgtcaa cgctgacttc cgaggtcgcg ccgttcacgc ggacaccggc ggaactcgca
      721 ccgttgttgg tcgagaagac gcagttgcgc aacgaagcga gcgagcgcag ggcggcttca
      781 aagccgtcgc cgcagttgtt catcatcacg ccgtcgagcg tggcggcgat gaggccgctg
      841 gcgttgctgg cattgagaat gacgccgttg cccacacagt ttctgatgga aacattcttc
      901 atgtagagtt cggtgacatt cgcgcccgcg atattgatgt caacgccgtc gccagtgagg
      961 ccgtcaatgt tcgtgttctc gatgtggagg gcaagaccgg cgatcatgcg tatgccgtcc
     1021 gtgccgctgc tcgcgccgtt gatgtccagc cagttgagac gcaccgtctt gcgtatgtcg
     1081 gctgcgtccg tgatgttgac gagaaaaccc tggggtgcga gcgcggagag caccgagccg
     1141 tagccctggc ctccgccgcc gccgttcacg gtgatcgatt tggtgatagt tagagtgccg
     1201 aagccgccgg ggtcgagcgt gctgatttcg ccgtctttag cggtcttgga gatcgcgccc
     1261 gcgtaagttt tgcagggggc ggtgcggctg cacgggttaa cgtcgtcgcc gacgcccgac
     1321 acccaggtgc gtgtcgcctg cgcctgagtc agggaggcaa gggcaaaaat gaagatgata
     1381 gaggtgagaa tactgagggt gaagcggcct ttattcattg ggaactctcc ttcgaagatg
     1441 ggaaatgctg ttttgtggtg attgttgata agacgtttcg tcacgttgcg ggtctacgtg
     1501 tgcgccgcat gcgagggcgg cggggttagc agttgaacta tcaagacaca gagaaaggat
     1561 gctcgtctcc ggcctccatt catgaagcgc gtagctttct atactaattt tctcaggctg
     1621 tcaatagttg gcagggagtc tcgaccgcga agctgcgcgc gtcgtctacg cgctcccgca
     1681 gcctgcgatt cttgacactg ttttgagcgc acacctatac tcgcacactt aatcggctta
     1741 actaatttcc ccgcaagcga cggtcaagca tttaaattta cgcagcgacg ggcgcgtgtg
     1801 tcgccgttaa attcgagttg agtcccggcg caacgcagcg cgagacttca cgccttcaaa
     1861 cacttcatgc aaaaattttt cgtcacgggc ggcgcgggct tcatcggctc ggcctttatt
     1921 cgacaggcgc tcgcgcgttg gcctgacgcc gagatcgtca acttcgacgc gctcacctac
     1981 gcgggcaatc cagacaacct ctccggactc gacgagacgc gccatcgctt cgtgcgcggc
     2041 gacatcaccg accgcgaagc ggtgcgcgcc gcgctcacgg aaggcacgga cgcgatcttc
     2101 aacttcgcag ccgagtcgca cgtagaccgc agcatcgagc gggcggatga tttcctgcgc
     2161 acgaacatcc tcggcacgca ggtcttgctc gacgccgcac gcgaaggcgg cgtccgccgt
     2221 ttcgtgcata tctcgacgga tgaagtgatg ggcagtctgc cggaagacga gagcgcgttc
     2281 ttcacggaag agtccggtta tgcgccaaac agtccttacg cggcttcgaa ggcggcagcc
     2341 gaacatatcg tgcgcgccgc gcgccacacc ttcggactcg acaccgtgac gacgcgctgc
     2401 ggcaacaact acgggccgcg ccagtttccc gaaaagctga tcccgctttt gctctcaaac
     2461 gcgctcaacg acgaacccat ccccgtctat ggcgacgggc tgaacgtccg cgattggatt
     2521 tacgtcgaag accactgccg cgccatcatg ctcgcttacg aacgtggcga agcgggcggc
     2581 atctacaaca tcggcgcgcg caacgaacaa cgcaacatcg aagtggtcga atcaatcctc
     2641 gccgcgctcg gcaaacccgc gtcgctcata cgctatgtca aggatcgtcc cggccatgac
     2701 cggcgttacg cgattgaccc ggcgaaggcg gagcgcgaac tcgactggcg acccgtcgag
     2761 acgtgggaga gcggcctcgc caagacgatc cgctggtacg aggagaacac cgtgtggatc
     2821 gcgcgcgtga ggaacggcgc gtatagagat ttttatgcgc ggcagtatgg cgtggaggtc
     2881 ggcgcaaagt gaaagttttg atcacggggg caggcggaat ggtcgcacgc gcgctcgcgg
     2941 cgcattgcgc gtcgcgcggc gatacggtct tcgcacacac gcgcgaaacg ctcgacatca
     3001 cgaacgccgg gaacgtgcgc gaagttttcg cacgcgacca gccggagacg gtgatcaact
     3061 gcgccgcctg gacggatgtt gacggttgcg aactaaatcc cgaacgcgcg gtcttggtca
     3121 acgccgaggc ggtcgagatt ctggcgactc acagccggat ggcgggcgca tcgttcgtca
     3181 cgatctcgac cgattacgtt ttcgacggcg caaaggaagg cttctacacg cagcgcgacg
     3241 accccgcgcc gcagagcgcc tacggcattg cgaaacttca gggcgagcgt cgcgctttgc
     3301 gcgcctcggc gcgaaccgtc gtcgtgcgtt caggctggat tttcggcacg ggcggcagaa
     3361 attttctcgc caccgccgtc gaacgcgcgc ggcgcggcga acatctcaag gccatcagcg
     3421 acgcctacgg cacgccgacc tacgcgccac atctggcggc gcgtttgcgc gaactggccg
     3481 agcgcgatct tcccggcacc tatcacgtcg tcaatagcgg cgcgggcgcg agtttctttg
     3541 agtttgcgca ggaggccgtg cgtgcggcgg gggtggaagg cgtggaggtc gagagcgtcg
     3601 catcggacac gctcgcgcgt cccgcgccgc gcccgcgcaa ttcgcgtctg cgctgcctgc
     3661 tctcggaagc catcggcctc gcacctctgc ccgagtggcg cgaagcgttg cgcgagttcg
     3721 ccggagcggg aaagacggaa ggagaagagg gaaagggcaa aggttagagg gcaaaccggg
     3781 gagaaaacag gcggcgcacc agatcgcttc tccttttccc tgagcctctt ccccttttcc
     3841 ctcttgtctt tacgccttca tccttctccg catctctgtt attatgcgtc gtttgttagc
     3901 gtccccgacc gagttggtat ttctcaagtt ttaaggttga aagaagatgt cccaagataa
     3961 tcgcctgctg ccgttgactc ccggtacgaa cctcgaccgc ccgttgcgcg aactgacgca
     4021 acccgagagg ccgaacaaca cctacgcgct cgccgagccg acgcacttgc gcgactatct
     4081 ccaagtcgtc ttaaaacgca agtggctcat cctgagcttg atcgtcgtcg tcacgtcgct
     4141 ggtggcgatc cagatgtatc gcatgccgac gatctaccgt tcggagtcaa tcattcagat
     4201 cgagcagaag aagacgaacc ttttgaagac gaaagagttt gtcctcaacg cgcagaccga
     4261 cccggcctac tggggcacgc aactcaagct ccttgagaat ccgcgcctcc accgcaaagt
     4321 gatcctcgcg ctcgacctgc aaaacaatcc ttcgttcgtc ggcggtcagg cgcgcccgag
     4381 tctgttcgcc accgtgcgcc gcatcttctc gcgcgagaaa ccggcggcca acgccccggc
     4441 agccgcgccc gtgggcggcc tcgacgtggt taacgacgcg cctgcggcca ccaccacggt
     4501 cgagacggag aacctgtcgc cccagcaggc cacgaagctg ctcgcctatg aagacatcat
     4561 gcgggctggc ctgaccatca ctccggtcga gcgcaccaac ctcgtcaaga tcgtctacga
     4621 gcacaccaac cctgaactcg ccatgaagat ttcggacacg ctcgccgccg tcttcattca
     4681 ggacaaccgg gaacgcgaaa ctttcggctc ggaaaagtct gccgaaagtc tcgccaagaa
     4741 catcaccgac ctgcaaatcg cctaccggca gaagcaggaa gagatcatcg ccttcaaacg
     4801 cagccacaac ctgccgctcg gcgcggcgga cggtcaggat ttgacgaaag cgatcctcgg
     4861 catgtccacc actcagatga tgcaggccga agacaagcgc aaggagttgg aagcctccta
     4921 ccgcgtggcg cagaacacgc aggacatcta ctccattccg gaggtgatgg ctaacaagtc
     4981 tgtacaggag ttgcgcgaca agttgagcac gcttgaggaa cagcgtcagg cattgctcgt
     5041 gcagtacacg gaagagtggc ctgccgtgaa gaagatcgac aagcagaagg cgcaggtgca
     5101 ggcttcgctc gataagcagg cgcgcgacgt ggtgcgaacg attgaaggca gttacaaagc
     5161 ggcggtgatg cgcgaaaact cgctccgtca gtcgaactct acggcgcgcg ggcaggcgaa
     5221 cgcgcaaagc cagtccgaag gtcatctcga catcaagctg caagagctgg aaacgatcaa
     5281 acaactctat aacaccgcgc tacagggcaa gaaggaattc gacgtgtcgg gcaacgaaac
     5341 gccgaacaac atcaccgtct ccacgcccgc gcggcgtgcg gagatcgtcg ggccgccgcg
     5401 tgtgcgcaac atcctgctcg gactgttgct ctctttggcg gcgggcatcg gcctcgcctt
     5461 cctgctcgac tacctcgacg acacgctcaa gtctatcgaa gacgtggaca ggcacataca
     5521 cctgccgacg ctcgccctga tccctgcccc gcgcgccgaa cgccgcttca tgcctgcgcg
     5581 cgcggcgctc gcttcgccgc cgggcggaga agccccgccc actgcgctgg cgctcatcga
     5641 agagaagcgt tcgcccgtgg cggaggctta tcgccacctg cgcacgtcgc tgctgctctc
     5701 ttccgcggga caaccgccga agaccgtgct cgttacttcg agtcaaccgt ccgaaggcaa
     5761 gacgaccacc gccgtcaaca ccgccgtcat gctcgcgcag acgggcgcgg aagtgctcat
     5821 tctcgattgc gatctgcgcc gcccgcgcat ccacgcgcat ttcggtctac agaacacgcg
     5881 cggcgtgacc aactacctgt cgggcgaggc caacatcagt tcgctcatac aggaatacga
     5941 caagctgccg aacttgaaga tcatcacttc cggtcccgtc ccgcccaact cggcggaact
     6001 cttaggctcg gaagagatgc gccgcctgct cgtcctgctg agcgataact tcacgcacat
     6061 catcatcgac tcgccgcccg ccatctcttt cacggacgcc tcgattcttt cgacgatggt
     6121 agacggcgtg atgctcgtcg tgcacggagg ccgctcgtcg cgcgccgtgg tgcgccgcgc
     6181 caagcagcaa ctcctcgatg tcggcgcgca catctacggc atcgtcctca acaacgttaa
     6241 gctcgaatca tccgactact actattactc cggctactat tcgagctact acgccaacga
     6301 agacgaagac cagaccgtcg ccaccgaagc ggacgacatg gtcgaaacgg ccaaatcgaa
     6361 ataacgagtc ccgcagttcc gaagtcccgc agtcccgagt cttaaaacag cgtctttgac
     6421 ttgggactgc gggacttcga gacttggggc tgcgagattt tatgagagaa ttttgcgcca
     6481 tcgccgacga cgtagtgctc gggcgcgatg tgcagattta caacttcgtc aatctctacg
     6541 gctgcgagat cggcgacgag acgcgcatcg gcaccttcgt ggaaattcaa aagggcgcac
     6601 acgtcggagc gcgctgcaaa atttccagcc acacttttat ctgtgaaggc gtgcgagtcg
     6661 aagacgaggt cttcatcggc cacggcgtac agttcatcaa cgaccgttac ccgcgcgcca
     6721 cgcgcgccga cggcacgccg cagaccgaag ccgactggac gctcgaacgc accatcgtcc
     6781 ggcgcggcgc atccatcggc acgggcgcga ctattctcgg cggcatcgag atcggcgcac
     6841 gcgccacggt cggcgcaggc gcagtcgtca cgcgcgacgt gcccgccgga gccaccgtcg
     6901 caggcaaccc tgcacgaatt ctaaaacagt gacgagtgac aggtgacaag ttaaaacagt
     6961 gaagcgtgac aggtgacggg tgacgagttg aagccaggaa cttgtctttc atcctgtcgc
     7021 ttgtcgttcg tcactcgtcg cccgtcaccc ttttaacttg tcacccgtca ctcgtcactt
     7081 gtcacggctc ttatgattcc atttgttgat ctgcaagcgc agtatcgcgc catcaagctg
     7141 gaagttaatg aggcgattgc gcgcgtcgtc gagagcgcgg ccttcgttct gggtcgtgag
     7201 gtggaggcgt tcgagtcggc cttcgccggt tatctcggcg cgcgtttttg cgtcggcgtg
     7261 agcaacggga cggcggcgat ccaactcgcc ctcatggcct gcggcatcgg cgcgggcgac
     7321 gaagtgatcg tccccgccaa cacgttcttc gccactgccg aagccgtctc gaccgcaggc
     7381 gcaacgcccg tcttcgtcga tgccgacccc atctcataca cgattgatgt caacaaaatt
     7441 gaagccgcga tcacggagcg tacgcgcgcc atcatccccg tgcatctcta cggtcaggca
     7501 gccgacctcg acgcgatctt cgagatcgcc gcgcaacgcg atctcgccgt catcgaagac
     7561 gcggcgcagg cgcacggcgc gctctacaag ggcagtcgcg tcggcgcacg cgggcgcgcg
     7621 aactgtttca gcttctatcc cggcaaaaat ctgggcgcat acggcgaggg cggcgcggtc
     7681 gtaacgaacg acgcggaagt ggcgcggcgc gtgcgactcc tgcgcgacca cggctcggag
     7741 cagaagtatc gacacgagat catcggctac aactttcgcc tcgaaggaat tcagggcgcg
     7801 gtcttaaacg tcaaactaaa atatctggac ggctggaacg atctgcgccg cgcgcacgcc
     7861 gcgcgttacc gcgagttact ggccgaggcg caccccggcg gcagcgacga cgacagcggc
     7921 acgctcacgc tcccgcagga gtcgccttac gcgcgccaca tctatcatct ctacgtcgtg
     7981 caaagcgttg agcgcgatgc gctgcaaaag cacttgagcg cggcgggaat tcagacgggc
     8041 atccactacc ctgtgcccgt ccacctgcaa cccgcttacg cgtcgctcgg gcacaaaccg
     8101 ggcgacttcc ccg
//
LOCUS       DASWNS010000629         9363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_257656, whole genome shotgun sequence.
ACCESSION   DASWNS010000629 DASWNS010000000
VERSION     DASWNS010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257656"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VGB76_21865"
     CDS             complement(<1..1319)
                     /locus_tag="VGB76_21865"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0101589.1"
                     /translation="MFRLVSFVVTVLLVGTTAYAQDKTAEIDKVFSWAKADAPGCAVA
                     VSEQGKLVVNRAYGSADLERDVPLNPASVFDAGSLRKQFVAATVLLLIEEKRLSLAED
                     IRKYLPELPDYGHKITLDHLLTHTSGLRDWTGLLPLAGGDPDALTIILRQRGLNFAPG
                     EEWSYSNSGYVLLTEIVARTSGMPFSAFTRKRLFEPLGMKTTTYIDDLRSVVKNRALA
                     YEKEGNRWRLDMLLGNDRGGGGALLSTASDLLIWNDALTNARLGAFVSEKLQEPARLN
                     NGRQISYARGLTVEPYGDGKMVWHSGGAAGYHSWLGRLPAQSLSIAVMCNSDAMAATA
                     LAHRVANQFLPAAPAPAPEAGAPPALPADALPEVNAKSGLFFNEQTGEPMRLVVDRGR
                     FRVAGGPGLVPLSKDRFRRWGASVQFMSQDEFELHFLSPDQFELKSM"
     gene            complement(1320..1442)
                     /locus_tag="VGB76_21870"
     CDS             complement(1320..1442)
                     /locus_tag="VGB76_21870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101590.1"
                     /translation="MKLHIRDVSKTYSNGVQALSGGADLRHSRPGSFPRFKWRI"
     gene            complement(1700..2293)
                     /locus_tag="VGB76_21875"
     CDS             complement(1700..2293)
                     /locus_tag="VGB76_21875"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY0101591.1"
                     /translation="MQHIWRKRIKRVLLLTALPIMLAFTLAASLIVADGLTDDIHAAD
                     VAIVPGNTVEKDGRPSARLRARLDQTVALYRQGLFPNVIVSGGVGSEGFDEAEVMKRY
                     LVENGVPDNRIHVDSGGATTHLTARNAARMMRENAWQSAMVISQYFHVSRTRLALKRS
                     GVTPVFSAHARYFELRDLYSIAREVVGYGTYLLRADY"
     gene            complement(2452..3021)
                     /locus_tag="VGB76_21880"
     CDS             complement(2452..3021)
                     /locus_tag="VGB76_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101592.1"
                     /translation="MKAMHALVLPLILLTAVSTAAQGKLKIDPENRYLLLSTVKTSTM
                     QKELDEASAQGFRIISAASSCGQSEMVLFLERVTQPPDTYKYRLLATTRTSTMEKELN
                     QAAQEGFRLLPRTITAKEGFLSNEIVSVLEQTPKSSKRYQYRLLATNRTSTLQKEVAQ
                     SEADGFVLVGLVGRGENMVIMEKEAQAKE"
     gene            3474..5498
                     /locus_tag="VGB76_21885"
     CDS             3474..5498
                     /locus_tag="VGB76_21885"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEY0101593.1"
                     /translation="MRSTRLSPYFRLLSLVLLVTLFAAGNVSAQTTGFTYQGRLTEGT
                     TPPNGTYEMEFKLYDTPTGGTQQPQPNPITIALTAANNSAVTVTNGVFSVQLDFGASA
                     FPGAARFLEISVRHNSGEAFTVLSPRQQITSTPYAIKSLNAETAATATSFSGSLTGDV
                     TGTQTTTVVGTVGGVSAADVASATSASNAATSSNTASAIVKRDASGNFSAGTITATLD
                     GNAASATTATTATTATTATTASGVSASAGNSVVTAINAGSSSVNTAHVAGDVELAPSA
                     QQTTSSTNHLINLKLVSTDPLNHKLGAPGQNALLSLSASGTYRDNSTHDKERFRVDND
                     GSILSVADYTGGINTTAPIEGSGTRFMWYATKAAIRAGEINGTQWDDANIGLYSTAFG
                     QNVRALGDNSIVVGKNSVAANTGTVALGEGHTATGANSVALGYGASTSTINGSPRTGT
                     FVFSDRSTLLTYTFNSTSPDIESQFHPTVTNSFNVRAVNGSFFYTNTALTTGFIFNST
                     TATMTLNNSKLTMASDGSTTLTSNSGGSAGVTLPAGGGSWNNLSDRNMKANFAAVNPR
                     AILRGVLALPISTWNYKSQGASVRHIGPMAQDFFATFKLGEGDKTISTIDPDGVALAA
                     IQGLNEELKDRDARIDTLQEQLRQQQGQIESLKKLLCLSHPGAQVCKESR"
     gene            5504..7321
                     /locus_tag="VGB76_21890"
     CDS             5504..7321
                     /locus_tag="VGB76_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0101594.1"
                     /translation="MRNSNSVIIILLLLIAASATHAQSVPQLTQSAVVGGGGASASGA
                     FRVEGTIGQGAIGESSGGAFSISGGFQAAVSGAASPSLTISDVTRAEGNSGQTTFIFN
                     VSLDSPAPTGGITFDIATSDATATDADNDYEPNSVNGATISEGASTYQFAVQVNGDMS
                     KEINETFNVNVTNVTGANVADGQGTGTVLNDDGAPSTGQIIISEFRLRGADPDGAGAE
                     TGELDEFIELYNNSDSDFIIVDSSPLPALASRGWAIVSDDPLNADPSELLTAKFTIPV
                     GTLIPARGHYLITNGLGYSLSLSEYMAADVTPQESGGSPASYFTDIRDGSGLALYRTG
                     NPAMFTPGERLDAVGFTGSTFFEGTPLTPAGGINTPLQHSFVRKLTSGLPQDTGNNEA
                     DFYFVEVNGATSNGRTAILGAPAPENSLSPVQRNATIKPSLIEPTTASTAPPNRVRSG
                     QVLAGVPNAYGTLSIQRRFKNSTNTPITRLRFRVIDITTLNSPVASSPQADMRVLSST
                     GVVTNSQGQEVVTVTGLAVEQSPAQPDGGGLNSTLTVVLPGGMLAPGNTIDVQFLLGV
                     QQQGSFRFLVNVEALTGAAVTSPNANRKTAGAKATGRGQ"
     gene            complement(7567..8517)
                     /gene="bla"
                     /locus_tag="VGB76_21895"
     CDS             complement(7567..8517)
                     /gene="bla"
                     /locus_tag="VGB76_21895"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HEY0101595.1"
                     /translation="MMRSIYGVIVMTQNNSFQIWRKRSLLCLACLFFIAPFFIECRNA
                     RAQADENFRSWNQPVKPFRIIGNVYYVGASDITSFLIATPEGHILLDSGFAETVPQIR
                     QNIKTLGFRLEDVKFLINSHAHIDHAGGLAELKELTKARLLASAADAALLANGGKGDF
                     GLVGDRFSFKPVKADRILRDGDKVKLGGTILTARLTPGHTKGCTTWTMKVSEKGRNYN
                     VVFVGSISAPGYRLIDNAAYPNIVADYEQTFRRLKKLPADIFLGSHGRFFGLQEKIKL
                     LAQNSDQNPFVDPNGYKDYLERAEKDFRAQLKEQQKKDQE"
     gene            complement(9009..>9363)
                     /locus_tag="VGB76_21900"
     CDS             complement(9009..>9363)
                     /locus_tag="VGB76_21900"
                     /inference="COORDINATES: protein motif:HMM:NF020826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101596.1"
                     /translation="ASLDPTLFLPGSLMLFFPLVKSRHTRPLFRTPDEETFFLFDILR
                     TIPHEAVASVLAENRRLYEQSRGLGSRFYTISAVQMEPHDWQEHFRPVWGRLVSEKRR
                     HDPDNVLGAGTRVFN"
BASE COUNT         1963 a         2907 c         2680 g         1813 t
ORIGIN      
        1 tccatagact ttagctcgaa ctggtctggg gacaggaagt gcaattcgaa ctcgtcctgc
       61 gacataaatt gcacggaggc tccccaacgg cggaaacggt ctttgctcaa cggcacaagg
      121 ccgggtccac cggcgacgcg aaatctccca cggtctacca ccagacgcat cggctcgccc
      181 gtctgctcat tgaagaacag gccactcttg gcgttcactt ccggtaaggc atcggcggga
      241 agcgcgggag gcgccccggc ctcgggtgcc ggcgcgggcg cggctggcag aaactggtta
      301 gcgacgcggt gggcaagcgc cgtcgcggcc attgcgtccg aattgcacat gacggctatc
      361 gaaaggctct gcgccggaag gcgacccagc catgaatggt atccagccgc gccgccggaa
      421 tgccacacca tcttaccgtc gccgtaaggc tccacggtca ggccacgggc gtagctgatc
      481 tgcctgccgt tgttcagcct tgcaggttcc tgaagctttt cgctgacgaa cgcgccgaga
      541 cgtgcgttcg tcagcgcatc gttccagatg aggaggtcgc tggccgtgct caagagtgcc
      601 ccaccgccgc cgcggtcgtt gccgagcagc atatccagcc tccagcgatt gccctccttc
      661 tcgtaagcga gggcgcggtt cttgacgacg ctgcgcaaat cgtcgatgta tgtggtcgtc
      721 ttcatcccga gcggctcaaa cagtcgtttg cgcgtgaatg cggagaacgg catcccgctc
      781 gtgcgcgcca cgatctctgt caacagcaca tagccgctgt tcgaatacga ccactcctcg
      841 ccgggcgcga aattgaggcc acgctggcgc aggattattg tcaaggcgtc tggatctccg
      901 cctgccagcg gcagcaaccc ggtccagtcg cggaggccgc tggtatgtgt gagcagatgg
      961 tctagcgtga tcttgtggcc gtaatcgggc aactccggaa gatacttgcg tatgtcttcg
     1021 gcaagagaaa ggcgcttctc ttcgatgagg aggaggactg tcgcggcgac aaactgcttc
     1081 ctcagcgatc cggcatcgaa gaccgaggcc gggttcaatg gaacgtcgcg ctcaaggtca
     1141 gccgagccgt aagcgcgatt cacgaccagc tttccctgct ccgagacggc gaccgcgcat
     1201 ccgggcgcgt ctgccttagc ccaactgaag accttgtcga tctcggcggt cttgtcctgc
     1261 gcgtatgcag tcgtgccgac aagcaagacg gtgaccacaa acgatactag tctgaacatt
     1321 caaatcctcc atttgaagcg tgggaaactc cccggtcggc tgtggcgcaa atcggcaccg
     1381 cccgacagtg cctgcacgcc gttggaatag gtcttggata cgtcgcgaat gtgtagtttc
     1441 attaaaacaa tcgtcgtttg aaaagtactt tgcaagataa agcaagcagg tgctcaagtc
     1501 aagtgggtcg ggttgcgcga cctgttggag cgtggctacg acatcaactc gtcgcgaata
     1561 aaagagcgag cggggcggcc atcaaactcc ggcaaccgtg cctgcaaaac ccctgccggg
     1621 caggtgctca ggcttaaact ctgtcccatc acgttgaatg agttgttgga tgaggcaatc
     1681 cgataaacta aaggccggtt cagtagtctg cgcgaagaag gtatgtcccg taaccgacta
     1741 cctccctcgc gattgaatat agatccctca gttcgaagta tcgcgcgtgc gcggaaaaca
     1801 ccggcgtgac tcccgaacgc ttcaacgcta acctcgtgcg cgagacatgg aaatactgag
     1861 agatgaccat cgcactctgc cacgcgtttt ctctcatcat ccgagccgca ttcctcgccg
     1921 tcagatgtgt tgtggcacct ccgctatcga cgtgaatgcg gttgtcgggc accccgttct
     1981 cgaccagata acgcttcatc acctccgcct catcgaaccc ttcgctgccc accccgccgc
     2041 tgacgatcac gttggggaac agcccctgcc ggtaaagggc gacggtttgg tcgagccggg
     2101 cgcgtagtct cgctgaaggg cgtccgtctt tttcgacggt gttaccgggg acaatagcca
     2161 cgtcggctgc gtggatgtca tcggtcaacc catcggccac aatgagcgag gccgccaggg
     2221 taaaggccag catgatcggc aaggccgtga ggagtagcac ccgcttaatt ctttttcgcc
     2281 agatgtgttg catacataga agtatctaac ggcaggcatc acccgacgcg cattcaacct
     2341 cgaacccaca caagctaacg gggagagacg tgccgattcg cggtcgagtg catgccgttg
     2401 tcgggcgcgg gttgtacaaa ataatcttcc ataccgggca ccgccgatac tctactcttt
     2461 agcttgtgcc tctttctcca taatcaccat gttctcacct cgacctacca atcccacgag
     2521 cacaaatccg tcggcttcag attgtgccac ttccttctgt agtgtcgagg ttcggttggt
     2581 ggctagcaga cggtactggt aacgcttgct cgatttgggt gtctgctcaa ggacggagac
     2641 gatctcgttg ctcagaaatc cctccttcgc agtgatcgta cggggcagga ggcggaagcc
     2701 ctcttgcgcc gcctgattaa gctccttctc catcgtgctc gtgcgcgttg tcgccagcag
     2761 ccggtacttg taagtatccg gtggctgcgt cacgcgttca aggaagagca ccatctcaga
     2821 ctggccgcaa ctcgacgcgg cagaaatgat gcggaagccc tgagccgagg cttcatcaag
     2881 ctccttttgc atggtcgagg ttttaaccgt cgagagcaaa agatagcgat tttccgggtc
     2941 tatcttcaat ttgccttgtg ccgctgtaga aacggcagtc agcagaatta agggaaggac
     3001 aagcgcgtgc attgctttca tctgtctgtc tcctgagaaa atggttagag caagtttgtt
     3061 ccgccttata ggataaggta tgcgtggttg gttagcacaa ccggacaccg ctcgaagcac
     3121 ccaacgacag ccatcacccg cgctctgttg ccatgacaga taaaagatct gctgcttggg
     3181 cgtcttgggt gcgtgccgtt gcaggcgtgc ctcgaaggat gccggagctt attttagtgg
     3241 ttgctttacg ctgccagaat attatattgt aatcaatagt gtttcggcct tcacctgtgg
     3301 ccgtttcctt tccagacctc tcggaacttt ccataaacaa gctcttaccg attttgtttt
     3361 cttgctccgt gactaagact gcttgaagcc tgcacagtaa aaccaccctg aacatttatc
     3421 gtttcgataa ttaacgtctg caaacaaatc tgtcgccttt gaggaatgta tccatgcgct
     3481 caactcgcct ttctccatac tttcgactcc tttctttagt cttactcgtt acactgttcg
     3541 ccgcaggcaa tgtttcggcg cagacgaccg ggttcactta tcagggcagg ttaaccgaag
     3601 ggacgacacc tcccaacggc acctacgaga tggagttcaa actctacgac acgccgacgg
     3661 gaggcactca acagccgcaa ccgaatccca tcacgattgc tctaacagcc gcaaacaata
     3721 gtgcggtaac ggtgacgaat ggcgtcttca gtgtgcaatt ggatttcggt gcgagcgctt
     3781 tccccggtgc ggcgcgattc ctggagatca gtgtccggca caacagcggc gaggcgttta
     3841 cagtcctctc cccgcgccag cagatcacct ccacacccta cgccattaag agtctgaacg
     3901 cggagacggc agcgacggcg acaagtttta gcggctccct cacaggcgat gtgaccggaa
     3961 cacagacgac cacggtcgtc ggcaccgtcg gcggcgtcag cgcggctgat gtggcgagcg
     4021 cgacgagtgc ttccaacgct gcgacgagca gcaacaccgc cagcgcaatc gtcaagcgcg
     4081 acgcctccgg caatttctcc gcaggcacca tcacagccac actcgacggc aacgccgcct
     4141 cggccacgac agccacgacg gcgacgacgg cgacgaccgc cacgaccgcc tcaggcgtca
     4201 gcgcctccgc aggcaatagc gtcgtcacag ccattaacgc tggcagttca tccgtcaaca
     4261 ccgcgcacgt cgcgggcgac gtggaactcg cgccctccgc ccagcagacg acgagcagca
     4321 cgaaccacct catcaacctc aaactcgtca gcaccgaccc gctcaatcac aagctgggcg
     4381 cgccggggca gaacgcgctc ctctcgctca gcgcgagcgg gacatatcgc gacaacagca
     4441 cccacgacaa ggaacgcttc cgcgtggata acgacggctc gattctgtcc gtcgccgact
     4501 acaccggcgg catcaacacc accgcgccca tcgaggggtc gggcacgcgc ttcatgtggt
     4561 acgcgacgaa ggcggcgatc cgcgccgggg aaatcaacgg cacgcagtgg gacgacgcca
     4621 acatcggcct ctactcgacg gccttcggcc agaacgtgcg cgcgctcggc gacaactcca
     4681 tcgtggtggg taagaactcg gtcgcggcca acaccggcac ggtggctctc ggcgaggggc
     4741 acacggcgac cggcgccaac tcggtcgccc tcggctacgg cgcgtccacc tcgaccatca
     4801 acggttcgcc gcgcaccggc acgttcgtct tctccgaccg ctccaccctg ctcacctaca
     4861 ccttcaacag caccagcccc gacatcgaat cgcagttcca ccccacggtc acgaactcct
     4921 tcaacgtgcg cgccgtcaac ggctccttct tctacacgaa cacggcgctc accacgggct
     4981 tcatcttcaa cagcacgacc gcgacgatga cgctcaacaa cagcaagctc acgatggcga
     5041 gcgacggctc gacgacgctc acgtcgaact ccggcggcag cgcgggcgtg acgctcccgg
     5101 cgggcggcgg ctcttggaac aatctctccg accgcaacat gaaggccaac ttcgccgccg
     5161 tcaacccgcg cgccatcctg cgcggcgtcc tcgccctccc gatctcgacc tggaactaca
     5221 agtcgcaagg agcctccgtc cgccacatcg gcccgatggc gcaggacttc ttcgccacgt
     5281 tcaagctcgg cgaaggcgac aagaccatct cgaccataga ccccgacggc gtggcgctgg
     5341 ccgcgattca aggcttaaac gaggagttga aagatcgtga cgctcggatc gacacgctac
     5401 aagagcaact ccggcagcag caggggcaga tcgagagctt gaagaaactg ctctgcctca
     5461 gtcatcccgg cgcgcaggtt tgcaaagaaa gcaggtgacg ttcatgcgca acagcaatag
     5521 cgtcatcatc atccttctcc tactcatcgc tgcgtcggcg acgcacgctc aatccgtccc
     5581 gcaactcacg caatcggctg tcgtgggcgg tggcggcgca agcgcatccg gtgcatttcg
     5641 agtcgagggc acaatcgggc agggcgctat tggagagtcc tccggcggcg cgttctcgat
     5701 ctccggtggc tttcaggcgg ctgtctccgg tgcggcttcg ccctcgctca ccatctccga
     5761 tgtaacccgt gctgaaggta actcagggca gaccaccttc atcttcaacg tctcgctcga
     5821 ctcacctgcg cccaccggcg gcatcacctt cgacatcgcg acctccgacg ctacagcgac
     5881 cgacgccgat aacgactacg agccgaacag cgtaaacggc gcgacgatca gcgagggtgc
     5941 cagcacctac cagttcgccg tgcaggtcaa cggcgatatg tcgaaggaga ttaacgagac
     6001 gttcaacgtc aacgtcacca acgtcacggg cgcgaacgtc gcagacgggc agggcacggg
     6061 caccgtcctc aacgacgacg gcgcgccttc aaccgggcag atcattatca gcgagttccg
     6121 cctgcgtggt gctgaccctg acggcgcggg agcggagacg ggtgaactcg acgagttcat
     6181 cgaactctac aacaactcgg actccgactt tatcatcgtg gactcctcgc cgctgcccgc
     6241 gctcgcctcg cgcggctggg ctatcgtctc cgacgatcct ttgaacgccg acccgagtga
     6301 actcctgacg gcgaagttca ccatcccggt cggcacgctc atccccgcgc gcggccacta
     6361 cctcatcacg aacggtctgg gctacagcct gagcctgtcg gaatacatgg cggctgatgt
     6421 tacgccgcag gagtcgggcg gcagccctgc ctcctacttc acagacattc gtgacggctc
     6481 aggacttgcg ctctatcgca ccggcaaccc ggcgatgttc acgcccggcg agcgactcga
     6541 cgccgtaggc tttaccggct ctaccttctt cgagggcacg cccctgacgc ccgctggcgg
     6601 catcaacacc ccgctccagc acagcttcgt gcgtaagctc acgagcggtc tgccgcagga
     6661 caccgggaac aacgaggccg acttctactt tgtggaagtg aatggcgcaa cgtcgaacgg
     6721 gcgcacggcg atcctcggcg cgcctgcgcc ggagaactcc ctgagtcctg tccagcgcaa
     6781 cgccacgatc aagccctcgc ttatcgagcc gacgacggct tcgaccgcgc cgcccaaccg
     6841 cgtccgcagc gggcaggtgt tggccggagt gccgaacgcc tacgggacgc tttcgatcca
     6901 gcggcggttc aagaacagca cgaacacgcc catcacgcgc ctgcgcttcc gtgtcattga
     6961 catcacgacg ctgaactcgc ccgtcgcgtc gagtccgcag gcagacatgc gcgtgctgag
     7021 ttcgacggga gtggtcacca actcgcaggg gcaggaagtt gtcacggtga cggggctggc
     7081 ggtcgaacaa tcgcccgcgc aaccggacgg gggcgggctg aactcgaccc tgaccgttgt
     7141 gctgccgggg ggcatgctcg cgcccggcaa cacgattgac gtacagttcc tcctcggagt
     7201 gcagcagcaa ggcagcttcc gtttcctcgt caacgtcgag gctctgacgg gggcagcggt
     7261 cacttctccg aacgccaacc ggaagacggc tggcgcgaag gctacaggca ggggacagta
     7321 agagaagcag tttaagcatt tcgcccgcgc cgcctgaggt caacgatggc ggcggaggcg
     7381 aatgaactga ggccgactga gcagtgaggg cttagtcggc ctcagttttt ttaagcgcga
     7441 tgtcaggcga cagcttgtga gtccttagca tacgttcgac gcagcgggag cagaggtgtc
     7501 tcaatcctta gttggcggcg tgcgccagaa gcgcgcatcc aatggggata agcgttagac
     7561 gcggcgttat tcttgatcct ttttctgttg ttcttttaac tgcgcccgaa aatctttctc
     7621 ggctcgttcc agataatcct tgtaaccgtt cgggtcaaca aacggatttt gatctgaatt
     7681 ttgggcgagc aattttatct tctcctgcaa tccgaaaaac ctgccgtgcg aaccgagaaa
     7741 aatgtcggcg ggcaattttt tcagacggcg aaatgtctgt tcgtagtccg cgacgatgtt
     7801 cgggtatgcg gcattatcaa tcaatctgta accgggcgca gatatactgc cgacgaaaac
     7861 gacgttgtaa tttcttccct tctcggaaac tttcatcgtc cacgtcgtgc agcctttagt
     7921 gtgtccgggc gtaaggcgtg ccgttaagat tgtgccgccg agtttaactt tatcgccgtc
     7981 gcgcagaatc cggtcggctt taacgggctt gaaagagaac ctgtcgccaa cgaggccaaa
     8041 gtcgcccttg ccgccatttg ccagcaaggc ggcatcggct gcgctcgcca acaatctggc
     8101 cttcgttagt tctttgagtt cggcaagtcc tccggcgtgg tcaatatgcg cgtggctgtt
     8161 gattaagaat ttgacatctt cgagccggaa gccgagcgtc ttgatgttct gcctgatttg
     8221 cggcacggtt tcggcaaagc cgctatcgag cagaatatga ccttcgggcg tggcgatcaa
     8281 aaaagaagta atatcggaag caccgacgta atagacgttg ccaatgatgc gaaaaggctt
     8341 gaccggctga ttccaggagc ggaaattctc atcggcctgc gcccgtgcgt tccggcattc
     8401 aataaaaaaa ggcgcgataa aaaagagaca cgccagacaa agcagagagc gttttctcca
     8461 aatttgaaaa gagttgtttt gagtcataac tatcacacca taaattgaac gcatcatcgt
     8521 aactaaccat tgcgcgcatc cccgcgcagt agaccctcat gaggtctgtc agccccaaac
     8581 tgtaaagctc acactgcgaa cggtcgccgc agaggttccc gaaacccctg cggcgaccgt
     8641 tcgcgctgtt ccccgccgcc acgcgccgcc cacaccgggg cgcggtaaga gccagagcac
     8701 taaacacaaa gatacaaagt cggcacaggc gcgtttacgg cggcgtctcg ccggtgagtc
     8761 tcgccaggcg taggcactcc gggcgtggtc atcacaggcg attattgaaa tcaatgtctc
     8821 gcaggagggg cttaccacag agcgttggcg cggcaactgc gcaagaagag ccggcagcat
     8881 tgacgcagcc gggcggttaa gttttacgga acaaggacgc acacgaccgc gaccgcgcaa
     8941 gtccagcccc ggctcgctca aacgccgggc gttggcgttt gagcgctacg tcagtgtgtg
     9001 cgcgggagct agttgaatac gcgcgtccct gcgccgagga cgttatcggg atcgtgcctg
     9061 cgcttctcgc tgacgaggcg tccccagacg gggcggaaat gctcctgcca gtcatgcggc
     9121 tccatctgca cggcgctgat ggtgtagaac ctactgccca ggccgcgact ctgttcgtag
     9181 aggcgtcggt tctcggcgag cacagaggcc accgcctcgt gcggtatggt gcgcaggatg
     9241 tcgaacagga agaaagtctc ttcgtcgggc gtgcggaaga gagggcgtgt gtgacgcgat
     9301 ttgacgagcg ggaagaagag catcagcgaa cccggcagga agagcgtcgg gtcgagcgat
     9361 gcg
//
LOCUS       DASWNS010000630         4820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_206164, whole genome shotgun sequence.
ACCESSION   DASWNS010000630 DASWNS010000000
VERSION     DASWNS010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206164"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VGB76_21905"
     CDS             <1..433
                     /locus_tag="VGB76_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101597.1"
                     /translation="VTCAPGSPITRAVEVAIVPLVGAEVIAGSTRLKAGTAQKLVLNM
                     LSTATMIRLGYVTGNRMTNMSARNEKLHARALRIFQAETGLDEQAAQAAFNAAGNDLR
                     VALVMHRTGRTRVEAEGALASSQGVVGQAIEQLLQSGEHLS"
     gene            523..1236
                     /locus_tag="VGB76_21910"
     CDS             523..1236
                     /locus_tag="VGB76_21910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0101598.1"
                     /translation="MKAQTSLHPSLRGVRAVLFDMGGTLVHPDWERLARLAAGEAGRE
                     FAAAELGRKFKEMMCEVDAGLQRGEPPPEDSKKRNWVFRRVYRALNMDDAACEKLSGL
                     LDATHAERHLWSAPDREAPAVLDALKAAGLRIAVISNTEDGRLEELLDLVELKAHFDF
                     FIDSYIVGARKPDAAIFQLALSRLELEPAEATYIGDSYGHDALAALAVGMRAVLLDPL
                     GLYPESVCPRIRTLGELIG"
     gene            1364..3079
                     /locus_tag="VGB76_21915"
     CDS             1364..3079
                     /locus_tag="VGB76_21915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101599.1"
                     /translation="MSFRRASLRALSALCLSISFGVAAFAQVTQPNGALGQVDFPTSG
                     SKEAQAHFLRGVAALHSFWYEEALEAFQAATKVDPDFMLGYWGEAMAHNHPLWNEQDT
                     EAARKVIVKIRDTGRLTARESKYLNAVKALYGEGDKHARNLAYVAAMEKIYREHPDDL
                     EAASFYALSLLGAVRPADKGYRLQVQAGAIALDVYEKNPNHPGAAHYIIHAFDDPEHA
                     ILALPAARRYAEIAPDAHHARHMPSHIFIQLGMWPEAAASNESSWATSDAWVKRKNYS
                     IERRDYHSLSWLEYVYLQQGRTREAEAQLALMQRSIADLGAPIRGYPYMAAAFIAETE
                     RWDLIDKHFGAPKSAALSTQKEAGADSCHAAAAKPAATASVIPVARTSQTTRPPFFQR
                     LYGAYVRGLSAAASKNPADAEKYAVELRELRVELEKLNSYFSPEYIEIMGLEVSALAR
                     ANAGKFDEAFELLRRATTLEESLRPPSGPPEIIKPSHELFGDILLRANRPAEAAAQFK
                     TALLRQPNRPRSLLGLARAAAKNGDTKLAAQTYAGYLHTQASADTHAAALNEARDYLK
                     QASTR"
     gene            3184..>4820
                     /locus_tag="VGB76_21920"
     CDS             3184..>4820
                     /locus_tag="VGB76_21920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEY0101600.1"
                     /translation="MRALDLFIIFGYLVGITAFGVWFSGKQETTRDYFLGDRTVPWWA
                     IAASIVATETSTITFISVPGIAFARGGNFQFLQLVFGYLLGRIVISLLFIPSYFRGEL
                     LTVYQLLERRFGLKIKMLAAALFVVMRNIADGVRLLLTAIVLAAVYVAFQPDANAEMI
                     TVYSIILLGAVMIIFTYFGGMEAVIWVEVVQLGIYIAGALAAAVVLAGAINGGFDGAL
                     ALGAQFDKFDVVDWGWRATKYTIFSPTSFWQALPFSDWSFAFQLPFDLTKTYTFWAGL
                     IGGCFLTMSTHGTDQYLVQRYLCTDRPRRAAAALLSSGGVVLAQFIGFLFIGALLFAF
                     YQPHNDPLYGNFAGACARLQPASAAKCLSDPAFAGATTANFPFTGGDRVFPDFITKHL
                     PSGLSGLVVAAIFAAALSSSLNAIAATAVNDLYKPFARGRNDRHYLRVSHWLTLGWGI
                     VQIAVALFFMRRNRSALDQALSVAGLINGPVLGIFLVGTFLKRVSEPPALVGMLVSIA
                     VMLYVRFMTPLAWTWYVLLGSLITFVVAYAVSFAFAPA"
BASE COUNT          866 a         1556 c         1464 g          934 t
ORIGIN      
        1 cgtcacgtgc gcgccggggt cgccgattac gcgcgcggtc gaagtggcga ttgttccgtt
       61 ggtcggggcg gaagtgatcg cgggttcgac gcggctcaag gcgggcacgg cgcagaagtt
      121 ggtgctcaac atgctctcga ccgcgaccat gatccgcctc ggctacgtca ccggcaaccg
      181 catgacgaac atgtctgcgc gcaacgaaaa attgcacgcg cgcgccctgc gcatctttca
      241 ggccgaaacc ggactcgacg agcaagcggc gcaggcagcc ttcaacgcgg cgggaaacga
      301 tctgcgcgtc gctctcgtca tgcaccggac gggacgcacg cgcgtcgaag cggaaggcgc
      361 gctcgcgtcg tcgcagggcg tcgtcggtca ggccatcgaa caacttttac aaagcgggga
      421 gcatttgtcg taagataggt tcactcgaag gcgagcgcgc gtaagcgtcc gccccctgat
      481 gaaaagattg aagccgggca catccttgat tccgcgattc aaatgaaggc gcaaacttct
      541 ctgcatccat ccttgagagg cgtgcgagcc gtgctgttcg acatgggcgg aacgctggtg
      601 catcccgatt gggaacgact ggcgcggctc gctgcgggcg aggccgggcg cgaatttgcc
      661 gccgccgaac tcggacgcaa gtttaaggag atgatgtgcg aggtggatgc agggttgcag
      721 cgcggcgagc cgcctcccga agattctaaa aaacgcaact gggtcttccg gcgcgtctat
      781 cgcgcgctca acatggacga cgccgcctgc gaaaagttga gcgggctact cgatgcgacg
      841 cacgcggagc gacacctgtg gagcgcgcct gaccgcgaag cccccgccgt gctcgacgcg
      901 ctcaaagcag ccggacttcg tatcgcagtt atctcaaaca cggaagacgg gcggctcgaa
      961 gagttgctcg atctcgtcga actcaaagcg cacttcgatt ttttcatcga ttcctatatt
     1021 gtcggcgcgc gcaaacccga tgcggcgatc tttcaactcg cgctctcgcg tctggaactc
     1081 gaaccggcgg aggccaccta catcggagac tcctacgggc acgacgcgct ggccgcgctc
     1141 gccgtaggca tgcgcgccgt cctgctcgac ccgctcggac tctatcccga aagcgtctgc
     1201 ccgcgcatac gcacgctcgg cgagttgatc ggatgaaatt ttgcggcgtg tggtcggtcg
     1261 cgtgcgcgag tttaaactga ggtcgaataa cctgaagtcg aatttgagcg tagcagcaac
     1321 agcgggaact ctctcctcga atcaagaaag ttgagatata gccatgtcgt ttcgtcgagc
     1381 atcgttgcgg gctttgtcgg cgttgtgcct ctccatcagt ttcggggtcg ccgccttcgc
     1441 gcaggtgacg cagccgaacg gcgcgctggg gcaggttgat ttcccaacct ccggctcgaa
     1501 ggaggcgcag gcacacttcc tgcgcggcgt cgcggcgttg cactctttct ggtacgagga
     1561 ggctctggag gcgtttcaag cggcgacgaa agttgatccc gatttcatgt tgggctactg
     1621 gggcgaggcg atggcgcaca accacccgct ctggaacgaa caggacacgg aagccgcgcg
     1681 caaagtgatc gtcaaaatcc gcgacaccgg acggctcacg gcgcgcgaga gcaaatatct
     1741 caacgccgtg aaagcgctct acggcgaagg cgacaagcat gcgcgcaacc tcgcctacgt
     1801 cgccgcgatg gagaagattt acagggagca cccggacgat ctcgaagcgg cctccttcta
     1861 cgcgctctct ctgctcggcg cggtgcgccc ggcggacaag ggctatcgtt tgcaggtgca
     1921 ggcgggggct atcgccctcg acgtttacga gaagaatccg aaccatcccg gcgcggcgca
     1981 ctacatcatc cacgccttcg acgatccgga acacgccatc ctcgccctgc ccgccgcgcg
     2041 ccgctatgct gagatcgcgc ccgacgcgca tcatgcgcgc cacatgcctt cacatatctt
     2101 cattcaactc ggcatgtggc cggaagccgc cgcctcgaac gagtcttcgt gggcgacttc
     2161 ggacgcgtgg gtcaagcgca aaaactactc catcgagcga cgcgattatc acagtctcag
     2221 ttggctcgaa tacgtctacc tgcaacaggg gcgcacgcgc gaagccgaag cgcaactcgc
     2281 cttgatgcag agatcaatcg ccgacctcgg cgcgcccata cgcggctacc cttacatggc
     2341 cgccgccttc atcgccgaaa ccgagcgttg ggatttgatc gataagcact tcggcgcgcc
     2401 caagtcagcc gccctctcca cgcagaagga agcgggcgcg gactcttgcc acgccgccgc
     2461 tgcgaagcct gccgcgacgg cgagcgtgat tcccgtcgcg cgcaccagtc agactacgcg
     2521 cccgccattc ttccaaagac tctacggcgc atacgtgcgc gggctgtcgg cagcggcctc
     2581 gaagaaccct gcggacgcgg agaagtatgc ggttgagttg cgcgaattgc gcgtcgagct
     2641 tgagaagttg aacagctatt tcagtcccga gtacatagag atcatgggct tggaagtctc
     2701 cgcgctcgcg cgtgccaacg cgggcaagtt tgacgaagcg tttgaactcc tgcgccgtgc
     2761 gacgacgttg gaagagtctc tgcgcccgcc ttcagggccg ccggagatca tcaagccttc
     2821 gcacgaattg tttggcgaca tactgctgcg cgcgaatcgc cctgcggaag cggcggcgca
     2881 attcaagacc gcgctcctgc gccagcccaa ccgcccccgc tcgctgctcg gactcgcccg
     2941 cgccgccgcc aaaaacggcg acaccaagct cgccgcgcaa acttacgccg gctacctgca
     3001 cacgcaagcc agcgccgaca cacacgctgc cgcactcaat gaagcgcgcg actacctgaa
     3061 acaggcgagc acgcgttagt gagcacgaag aatgttgtcc ggcgacaaga acttgacggc
     3121 gaaaggcgtt gcgccgtgga catctggaac gcggagcctg cgacctgact tcgacaggag
     3181 actatgagag cgctcgatct ctttatcatc ttcggctatc tcgtcggcat tacggcgttc
     3241 ggcgtctggt tctccggcaa gcaggagacg acgcgcgact attttctcgg cgaccgcacc
     3301 gtgccgtggt gggcgattgc tgcgtccatc gtggcgacgg agacttcaac catcacgttc
     3361 atctccgtgc ccggcatcgc ttttgcgcgc ggcggcaatt ttcagtttct ccaactcgtc
     3421 ttcggctacc tgctggggcg catcgtgatc tcgctcctct tcatcccctc ctactttcgc
     3481 ggcgaactac tcaccgtcta tcaactgctc gaacgacgtt tcggcctcaa gatcaagatg
     3541 ctcgccgccg cgctcttcgt cgtcatgcgc aacatcgcag acggcgtgcg cctgctgttg
     3601 acggccatcg tgctcgccgc cgtctatgtc gcttttcaac ccgacgcgaa cgccgagatg
     3661 atcacggtct attcgatcat cctgctcggc gcggtgatga tcatttttac ttacttcggc
     3721 gggatggagg cggtgatctg ggtcgaggtg gtgcaactcg gaatctacat cgcgggcgcg
     3781 ctcgccgccg ccgtcgtcct ggcgggagcg atcaacggcg gcttcgacgg agcgttggct
     3841 ctcggcgcgc agttcgataa attcgacgtg gttgactggg gctggcgcgc gacgaagtac
     3901 acgatctttt cgccgacgag tttctggcaa gccctgccgt tcagcgattg gagtttcgcg
     3961 ttccaactgc cgttcgacct gacgaagacc tacacgttct gggctggact catcggcggc
     4021 tgctttctca cgatgagcac gcacggcacg gatcaatatc tcgtgcagcg ttacctctgc
     4081 actgaccgac cgcgccgcgc ggcggcggcg ctcttgtcga gcggcggcgt ggtgctcgcg
     4141 cagttcatcg gcttcctctt catcggcgcg ctcctcttcg ctttttatca accgcacaac
     4201 gacccgctct acggcaactt cgccggagcc tgcgcgcgtc tgcaacccgc ctcggcggcg
     4261 aagtgcctct ccgatccggc cttcgccggg gcgaccaccg ccaattttcc tttcacgggc
     4321 ggcgaccgcg tcttccccga tttcatcacg aagcacctgc cttcggggct gtcgggtctg
     4381 gtcgtcgcgg cgatcttcgc cgccgcgctc tcttcgtcgc tcaacgcgat tgcggctacg
     4441 gcggtcaacg atctctacaa acctttcgcg cgcgggcgaa acgacaggca ctacctgcgc
     4501 gtctctcact ggctgacgct cggctggggc atcgtgcaga tcgcggtcgc gctcttcttc
     4561 atgcggcgga accgttccgc gctcgatcag gcgttgtccg tggcggggct gatcaatggt
     4621 cccgtgctcg gcatctttct cgtgggcacg tttctcaagc gggtgagcga gccgcccgcg
     4681 ctcgtcggca tgctcgtgag catcgctgtg atgctctacg tgcgttttat gacgccgctg
     4741 gcgtggacct ggtatgtgct gctcggaagt ttgatcacgt tcgtcgtcgc ctacgcggtg
     4801 agcttcgcct tcgcgcccgc
//
LOCUS       DASWNS010000631         4855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_283651, whole genome shotgun sequence.
ACCESSION   DASWNS010000631 DASWNS010000000
VERSION     DASWNS010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283651"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VGB76_21925"
     CDS             <1..700
                     /locus_tag="VGB76_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101601.1"
                     /translation="TWSSFIDTASEIFNPSGAEVAVSQDSFNRSSERARSSFDRPHRF
                     SGNFVYELPFHRDQQGFVGHVLGGWQMNSFFTFQSGSPFTVLQGTDPAGTLAGISGLV
                     GNSIRPNLNTTLPLGQMSVEEIRVAAGALTCGASAGAACLFTPVTAAQRVGNAGRNIL
                     RTDGINRVDFGILKNTRISESQRIQIRADFFNVSNTRDFGVPEGRINNSNFLNQWGTN
                     GGNRRVIVGLRYVF"
     gene            938..1561
                     /locus_tag="VGB76_21930"
     CDS             938..1561
                     /locus_tag="VGB76_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101602.1"
                     /translation="MKPLALAFLFVAAMSSGAFAQSVAETHDPPDLQVVKFGWSKERL
                     GWERDPFSGPVENFDQMRVRQRNERRISDAKRNGTSSDVDRITREAKADSANIEELRR
                     QQQPQRYGFLYKVSFKNNGTKIIKSLDWDHVFFDSQTKAETMRHQFTGDEKISPGKQK
                     EFTVFTPAPPSRTISVQKLDKRERDTLGEAIVIMRILYADGSVWQRP"
     gene            1703..2275
                     /locus_tag="VGB76_21935"
     CDS             1703..2275
                     /locus_tag="VGB76_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101603.1"
                     /translation="MRLPCKTNLEKAWLITSLSVLFLHRMSEASGSVYYSPAIIERLW
                     LEVSMIVLSFPFGGLLMFVLRDALSWCDGCRSLEWMLDWSTLLLAGYIQWFWVLPEIL
                     RRRQLTFLNLRQAEESVLPETDPASSLPAHNVSPDVSPVAATTAESLARLPAEFDEAG
                     RSALDRVLQSGWSTPPAPPAHVELIFPSVR"
     regulatory      2537..2772
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3069..>4855
                     /locus_tag="VGB76_21940"
     CDS             3069..>4855
                     /locus_tag="VGB76_21940"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0101604.1"
                     /translation="MRNTHTSKYSPAGKSLPLRKSVLARKFLAASGCCALVAFFLCAP
                     SLFAQTGATPNANASSGISGRVTDAQGASIAGAIVTLYTRAAQLRLTAVTDANGAYRF
                     ASLAAGDYLIEPDARGFAPAVAERVRVERGETTTLDFVLEVAGVSAQVVVTAADTPQA
                     VDEVSKAITTINERELEERDEYSIPEALRAVPGLRVQQLGGPGALVSIKTRGLRNQDT
                     AILFDGLRFRDPSSPQGDASGYLSDLHATNVSRIEVLRGSGSSLYGTNAIGGVVNIIT
                     DEGGGPLHGSVLAEGGGLGLFRGRAQVAGGTGASRVLYSAGVSHFNTTRGIDDDDAAR
                     TTSGQARAAFRLTPDATLTVRFYAADSFTQLNEDARPVSGALPAGIIRAVPLSRAELR
                     RFETGTPDFQLANGAATFIPSTNDGDASRATHFFSSALIFNARPTDNFGYTISYQGNR
                     TDGAFYDGPAGTGFEPFGTTRADFDGHADTLNARIDFRLGRANLFTAGYEFERERFIN
                     RSLSTAVPEDDYLVDVAERSHSFFIQDQVRLLQDRLQLSAGFRMQRFALSHPDFKPLG
                     TSPFANFDFAAPPNAYTGDGSIAYSFPATG"
BASE COUNT          870 a         1624 c         1419 g          942 t
ORIGIN      
        1 tacgtggagt tcgttcatcg atacggcttc ggaaatcttc aacccgtcgg gcgcggaagt
       61 cgccgtctcg caggactcct tcaaccgcag cagcgagcgc gcgcgttcga gcttcgaccg
      121 cccgcaccgc ttctccggca acttcgtcta cgagttgccc ttccaccgcg accagcaggg
      181 cttcgtcggc cacgtcctcg gcggctggca gatgaactcg ttcttcacct tccagagcgg
      241 ttcgcccttc accgtgctgc aaggcacaga cccggcgggc acgctcgcag gtatctccgg
      301 cctcgtcggc aactcgattc gcccgaacct gaacaccacc cttccgctcg gccagatgtc
      361 cgtcgaggag attcgcgtgg cagcgggcgc gctcacttgc ggggcgagcg cgggcgcggc
      421 ctgcttattc acgcccgtca cagcggcgca gcgcgtcggc aacgccggac gcaacatcct
      481 gcgcaccgac ggcatcaacc gcgtggactt cggcatcctc aagaacacgc gcatctcgga
      541 gagccagcgc atacagattc gcgcagactt cttcaacgtc tcgaacacac gcgacttcgg
      601 cgtccccgaa gggcgcatca acaattcgaa cttcctgaac cagtggggca cgaacggcgg
      661 caaccgccgc gtcatcgtcg gcctgcgcta cgtcttttaa ttgccgcgcg ggtcgggcgg
      721 cgactctctc aagcgcgcgg gagactcgcc gcccggctgt tcgcgctact tgccgggggc
      781 tggtgaagca cgcgcttcac cagccccgtc tgttacggtt caggctcgcc gccgcgtcac
      841 tttcctgcgc gctttctttc gcggtctgcc ggtagtattc tgccgctcac gggagatcaa
      901 ttgcatgcgc gtctccggct tgcgggagga agggattatg aaacctctgg ctctggcgtt
      961 tctctttgtg gcagccatgt cgtccggcgc attcgcacag tcagtcgcgg agacgcacga
     1021 cccgcccgac cttcaagtcg tcaagttcgg ctggtcgaaa gagcgtctcg gttgggagcg
     1081 tgatcctttc agcggcccgg tcgagaattt cgatcagatg cgcgtgcgcc agcgcaacga
     1141 gaggcgcatc tccgatgcca agcgcaacgg cacttcctcc gacgttgacc ggatcacgcg
     1201 cgaggcgaaa gccgactcgg cgaacatcga agaactgcgc cgccagcagc agccgcaacg
     1261 ctacggcttc ctgtataaag tctcgttcaa aaacaacggc acgaagatca ttaagtcgct
     1321 cgattgggac cacgtttttt tcgattccca gacgaaggcc gaaaccatgc gccatcaatt
     1381 caccggcgac gaaaagatca gccccggcaa acagaaagag ttcaccgtct tcactccggc
     1441 tccgccctcg cgcaccatca gcgtgcagaa actcgacaag cgcgaacgcg acacactcgg
     1501 cgaagccatc gtcatcatgc gcatcctcta cgccgacggc tccgtctggc agcgaccata
     1561 aaagccgacc gggcgcacac gcccgccgaa ccctcccggc gtgcgggaac aaaagcccgc
     1621 gtcgcgcgtc taacttctac gtcggatgat tctcgtcgca gattctccgg ctgcgattcc
     1681 aacgggcgta ggtgtgtgcc agatgcgttt accgtgcaag acaaaccttg agaaggcgtg
     1741 gctcatcacg agcctgtctg tgcttttcct gcaccggatg tcggaggcca gcggttccgt
     1801 ttattattct ccggccatca tcgaacggct ctggcttgaa gtgtcgatga tcgttctctc
     1861 cttcccgttc ggcggcctct tgatgtttgt gcttcgtgac gctctctcct ggtgcgacgg
     1921 ttgcagaagt ctggaatgga tgctcgactg gtcgacgctg ctgctcgccg gttacattca
     1981 atggttctgg gtgctgcccg aaattctgcg gcgcaggcaa ctcacctttc tcaatcttcg
     2041 acaagcggaa gaaagcgtcc tgccggagac ggatcctgct tcctctctgc ccgctcataa
     2101 tgtttcaccg gatgtttcgc cggtcgcagc tacgacggcg gaaagtctcg cccgtctgcc
     2161 cgccgaattc gacgaggcgg gtcgctccgc gctcgacaga gttttgcagt ccgggtggtc
     2221 tacgccgccc gcgccgcccg cccacgtcga attgattttc ccttcggtgc gctaaacccg
     2281 cgtgcgtgaa agttcccccg cttctgtgcc tgcaaagtcg tgcgcggcct ctctccggcg
     2341 tgctccgctt gaccttgcgg gtgcggggtg ataggttgtt catccgcgct gcgcataatc
     2401 taaaagactg ggagatgaac agcagcacgc ggcgcgaggt gtcgggcgcg tagcccgcat
     2461 cttgatcttt gtcactcgaa taatttgtat cgctattcaa ttcagacgtg ctctcgccga
     2521 cacaggcggc agcacgactg gattgaccgt cgcaggtgtg acccgcgcgc tctctgattt
     2581 gagagacgcg ggcgacaagg gaagccgggt ttaaagcccg cacggttccc gccactgtgt
     2641 accggcgatt gtgtcgcacg ccaatagaag tgcatggtca ctcgtctctg cattacagag
     2701 acggggaagg ccgcgcgagc gacgcgtctg agccggaagt caggaaacct tgcctgcatc
     2761 gaagagacgg aggcgaccgc tgacgctccg ggaaagggcg agtggctaaa ctcaaaagta
     2821 cgtgcacccg tcaaaccccg gcacgacgcg ctctcttcct cgttatctct aacttgaaga
     2881 accgcgccgc cccggtttga tacgatacca attttgaagc gccctcgatc ttgccacggc
     2941 aggatcgagg gcgtttctat ttgcaggttc gcgggcaggc gcgtctcttt gccagcctct
     3001 caaattttcg cgcgttgcgc ccgtccgtcc tccgccgccg aagttcacga gagaggatgc
     3061 gagaggaaat gagaaacaca cacacatcta aatattcacc ggctggtaaa tctttgccgc
     3121 tccgtaagtc tgtgctcgcg cgtaaattct tagcggcctc cggctgctgc gcgctcgtcg
     3181 ccttcttcct ctgcgcgcca agtctcttcg cgcaaacagg tgccacgccg aacgcgaacg
     3241 cctcttccgg catcagcggg cgcgtgacgg acgcgcaggg cgcgagcatc gcgggcgcaa
     3301 tcgtcacgct ctacacgcgc gcggcgcagt tgcgcctgac ggctgtgacg gatgcaaacg
     3361 gcgcgtaccg ttttgcaagt ctcgcggcgg gcgactattt gatcgagccg gacgcgcgcg
     3421 gctttgcgcc agcggtcgcg gagcgcgtgc gcgtcgagcg cggggaaacg acgacgctcg
     3481 atttcgtgtt ggaagtggcg ggcgtcagcg cacaggtcgt cgtgacggca gccgacacgc
     3541 cgcaggccgt ggacgaagtc tccaaagcga tcacgaccat caacgaacgc gagttggagg
     3601 agcgcgacga gtattcgatt cccgaagccc tgcgcgccgt gccgggcttg cgcgtgcagc
     3661 aactcggcgg tcccggcgcg ctcgtctcga tcaagacacg cggcctgcgc aatcaggaca
     3721 cggcgatcct gtttgacggc ctgcgctttc gcgatccctc ttcgccgcag ggcgatgcct
     3781 ccggctatct gagcgatctg cacgcaacaa acgtgagccg catcgaagtg ttgcgcggtt
     3841 cgggttcgtc actctacggc acaaacgcga ttggcggcgt cgtcaacatc atcacagacg
     3901 aggggggtgg acctctgcac ggaagcgtgc tcgccgaagg cggcggcctc ggactctttc
     3961 gcggtcgcgc gcaggtcgcg ggcggcacag gcgcatcgcg cgttctctac agcgcgggcg
     4021 tctcacactt caacaccacg cgcggcatag acgacgacga tgcggcgcgc acgaccagcg
     4081 gacaggcgcg tgccgccttt cgtctcaccc ccgacgcgac gctcaccgtg cggttctatg
     4141 ccgcagactc tttcacccaa ctcaatgaag acgcgcgacc cgtctccggc gcactgcccg
     4201 cggggatcat ccgcgccgtg ccgctctccc gcgcggagct tcgccgcttt gagacaggca
     4261 cgcccgactt ccagttggcg aacggcgcgg ccactttcat tccttcaacc aacgacgggg
     4321 acgcctcgcg cgccacgcac ttcttctcct ccgcgctcat cttcaacgcg cgtcccacgg
     4381 acaacttcgg ctacacgatc tcttatcagg gcaaccggac ggacggcgcg ttttacgacg
     4441 gcccggcggg cacgggcttc gagcctttcg gcacgacgcg cgcggatttc gacgggcacg
     4501 ccgacacctt gaacgcgcga attgattttc gcctcgggcg cgcaaacctc ttcactgccg
     4561 gttatgaatt cgagcgcgag cgtttcatca accgttccct gagcacggcg gttccggagg
     4621 acgattattt ggttgacgtg gccgaacgca gccattcctt cttcattcag gatcaggtgc
     4681 gcctcctgca agaccgcctg caactatcag ccggcttccg catgcaaagg ttcgccctct
     4741 cgcaccccga cttcaagccc ctcggcacct cgcccttcgc caacttcgac ttcgccgcgc
     4801 ccccgaacgc ttacaccggc gacggttcaa tcgcctattc cttccccgcc accgg
//
LOCUS       DASWNS010000632         8691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_206250, whole genome shotgun sequence.
ACCESSION   DASWNS010000632 DASWNS010000000
VERSION     DASWNS010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206250"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VGB76_21945"
     CDS             <1..1234
                     /locus_tag="VGB76_21945"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY0101605.1"
                     /translation="ERGELMNEVRALVEAFDGANERGERCALATVVSVEGSSYRRPGA
                     RMLVCEGGASTGTISAGCLEADVIEHAKRVIRTGEAVLVEYDTASTSDEMAWGLGLGC
                     NGVVRVLVEPLAPGSTYVEALRRSCRTREEASPVTVATVYQQTASGSASAAAARVATG
                     ARLFIDEGGAACREHLSDEAASLIEGDVRMTACAEPSGTRVYEVEGGSLKVFVETLLP
                     PVQLVIFGAGHDALPVVEFARGLGWQTEVVDPQARPACLSRFAPADRVTLSRPEEVGA
                     RVRITPQTLTLLMSHNYSHDLALLKFLLASPARYIGVMGPRKRTERMLCELAVSDGAF
                     SLREANMDRLHSPTGLDLGANTPAEIALSIITEMRAVLDGRRGGMLRERRGSIHGSPC
                     DAGRTRPAEEQSLTVAMA"
     gene            1269..1895
                     /locus_tag="VGB76_21950"
     CDS             1269..1895
                     /locus_tag="VGB76_21950"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0101606.1"
                     /translation="MVESNPVSIGAVVLAAGSSSRMGSPKQTLQFRGESLLRRAALAA
                     LGAGCSPVVVVTGAHARLSRRELHGLDVREVLNNLWETGMASSIRAGVEGVAGANPAV
                     DAAVLMLCDQPHVTAEIISALIAAHRATGSHVVASTYGGSFGVPALFSRALFAELAQL
                     EGADGAKQVIKRHASEAHFLPFPRGEVDVDTPDDFSRLTAKDVEPEQA"
     gene            complement(2028..3281)
                     /locus_tag="VGB76_21955"
     CDS             complement(2028..3281)
                     /locus_tag="VGB76_21955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101607.1"
                     /translation="MKGQASMFTFQNLKAVLLLTASLLAFESSVAAQSGRKPPPPAPT
                     PAPTPAPTLIDTTNLEKVKLLISRGLYDFIRDLNEQGRLGYRVEKSVSYGETGERRKY
                     AAVLRLDPGHTYAYAHDRMPVEESYGHPLNYNARRGYKLVETYAVVKCPPVEYSPEPK
                     PPWDLDTLLSTEKSSVFLFMRRDGVAEPTMEYRLLTGRVGWGENLQQTIQAGIDEAPP
                     GFRPVRLLFAGPGLASSGVSVVLERDMSEAAPVKIKYQVVKERRNLLKELSQFATAGA
                     RYIGGGRIDAFKVALIERRAAGLSDYTFIEDDEGVKGFDKMIAAGYSYQGLMGGDIKC
                     ESEEMVSQKLVFAREAAPATRRYKILSLPEPKPGSSPSPALSELQRLAGEDFQIRDIF
                     YAYRLYVILEKQTGARVSQAQAAAR"
     gene            complement(3861..4757)
                     /locus_tag="VGB76_21960"
     CDS             complement(3861..4757)
                     /locus_tag="VGB76_21960"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0101608.1"
                     /translation="MSTPDFNLLVTLDAVLAEGSVARAARRLRLSPSAMSRALARLRE
                     TTGDPLLVRAGRGLVPTPRALELRERVGQLVQDIEAVLRPAETPDLKQLVRTFTLRTS
                     EGFVENFGPELIARVGVEAPGVRLCFVQKPNKESTSLRDGTVDLETGVVGNTMGPEVR
                     AQTLFRDRFFGVVRIGHTLSKGKITPARYARGGHISVSRRGIDQGAIDEALKPFGLER
                     KIVTIVGGFSTALALARASDLIASVPERHTGNLRAGMHSFPLPFSTPELTVSLLWHPR
                     LDADPAHRWLRGLVLETCAAVR"
     gene            4863..6320
                     /locus_tag="VGB76_21965"
     CDS             4863..6320
                     /locus_tag="VGB76_21965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0101609.1"
                     /translation="MLKTIIEEQNEAGTGSAKLTPSVRWALASLSLSMLMPSLDTSIA
                     NAGLPTLAEEFDASFQEVQWIVLAYLLAITTLIVSAGRLGDIIGRRRLLLFGIGLFTT
                     ASLLCGVAPTLRLLIAARAVQGLGAAIMMALAVALVSETVPQAKTGSAMGLLGTMSAI
                     GTTLGPSLGGLLIAGLGWRMIFLVNVPLSILNFLLAHRYLPVARRAEKTERRAFDNVG
                     TLLLGVTLAAYALAMTIGRGHFGRLNIALLLAAVFGAGSFVLVEARVAKPLLRLAMFR
                     DPVLSASLAMSTLVSTVMMATLVVGPFYLSRGLGLETALVGLALSAGPLVAALTGVPA
                     GRLVDRFGAQRMTITGLVGIAVGSSVLSMMPSSFGVVGYIAAIVVITASYALFQAANN
                     TGIMTGIRPDQRGVISGLLSLSRNLGLITGASAMGAVFALASATTDITTAHPEAVATG
                     MRTTFAAAAILLFVALTISVGSRALSRRAVSLNPV"
     gene            complement(6423..7451)
                     /locus_tag="VGB76_21970"
     CDS             complement(6423..7451)
                     /locus_tag="VGB76_21970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0101610.1"
                     /translation="MSKVRTEDVVAMYTKYPYPSPTVGTSLSYDIANLFHFLCGKDAL
                     NNRKILDAGCGTGQRVLGFAKQYPKAEFLGIDLTEASLEVAEQLADKHGIHNITFKQR
                     NILELDLEETFDIIVSTGVVHHLEDPQKGLFNLCQHLTGDGVICLWYYHPFGEFERLV
                     GRELLLTLWDAQRSDLLKGQRIMEQLKLKLPPDQYGTTATQAERDRSQLSIDADAFLH
                     PIVNAYRFDEAMAMFKTCDVDWVAINGFNTLKTMKLVDLMQVEEEAQELCLWDTGLFE
                     EKELIDLYRALTKPDQLKVIELMTKPTGFTILAGKKDSFKKLGRRIEGNLVMKEDLPE
                     PYPRLLRV"
BASE COUNT         1738 a         2546 c         2549 g         1858 t
ORIGIN      
        1 agagagaggt gaactgatga acgaagtgcg agcgttagtc gaagcgttcg acggggcgaa
       61 cgagaggggc gagcggtgcg cgctggcgac ggtcgtgagc gtcgaaggct cttcgtaccg
      121 gcgaccgggg gcgcggatgc tggtctgcga aggcggggcg agcacgggca cgatcagcgc
      181 aggctgcctc gaagccgatg tcatcgagca cgcaaagcgc gtcatacgca cgggtgaggc
      241 tgtgctcgtc gagtacgaca ccgcctcgac aagcgacgag atggcctggg ggttgggact
      301 cggctgcaac ggcgtggtgc gcgtgctcgt cgagccgctc gcccccggtt caacctacgt
      361 cgaggcgttg agacgttcgt gccggacgcg tgaggaagcc tcgcccgtga cggtcgcaac
      421 cgtctatcag cagacggctt ccggctcagc ctccgctgct gccgcgcgcg tcgcaaccgg
      481 cgcgcggctc tttatagacg aaggcggcgc ggcctgccgc gagcatctaa gcgacgaggc
      541 ggcgtcgctc atcgagggcg acgtgaggat gacggcgtgc gcggagccgt ccggcaccag
      601 agtctacgag gtcgagggcg gctccttgaa ggtcttcgtg gagacgctcc tgccgcccgt
      661 ccaactcgtc atcttcgggg cggggcacga cgcgctgccg gtcgtcgagt tcgcgcgtgg
      721 tctgggctgg cagacggaag ttgtagaccc gcaagcccgc cccgcctgcc tctcccggtt
      781 cgccccggcg gacagggtta cgctctcgcg ccccgaagag gtcggcgcgc gggtcagaat
      841 tacgccgcag accctgacgc ttctaatgtc gcacaactac tcgcacgacc ttgcgctgct
      901 caagttcctg ttagcctcgc ccgcacgcta catcggggtc atgggtccac gcaaacgcac
      961 agagcggatg ctgtgtgagt tggccgtgag cgacggcgcg ttcagtctcc gcgaggcaaa
     1021 catggatcgc cttcactccc cgaccggtct ggacctcggg gcgaacactc ccgccgagat
     1081 cgcactgtcg atcatcaccg agatgcgcgc ggtgctggat gggcgacgcg gcggaatgtt
     1141 gcgcgagcgg cgcggctcca tccacggcag tccgtgtgac gccgggcgga cgcgcccggc
     1201 ggaggagcag tcccttactg tggcgatggc atgagaggcg tcaggccgac gcggagggcg
     1261 attccgagat ggtcgaaagt aacccggtga gcatcggggc ggtggtgctg gcggcggggt
     1321 catcctcgcg catgggcagc ccgaagcaga ctcttcaatt ccggggcgag agtctgctca
     1381 ggcgcgccgc cctcgcggcg ctgggcgcag gatgcagtcc cgtcgtcgtg gtgacgggcg
     1441 cacacgcccg gctgtcgaga cgcgaactgc acggcctcga cgtgcgggaa gtcttgaata
     1501 acctttggga gacggggatg gcatcgtcga tacgagcggg cgtcgaaggt gttgccgggg
     1561 cgaacccggc ggtggatgca gccgtcctca tgctgtgcga ccagccacac gttacagccg
     1621 agatcatctc cgccctcatc gcggcgcacc gcgcgaccgg aagccacgtc gtcgcctcga
     1681 cgtatggcgg gagcttcggc gtgcccgcct tattcagccg tgcgctcttc gccgaactgg
     1741 cacaactcga aggagcggac ggcgcaaagc aggttattaa gagacacgct tcggaggcgc
     1801 acttcttacc cttcccgcgc ggtgaggtgg acgtggacac gccggatgat ttctcccgcc
     1861 tgactgcaaa ggatgtcgaa ccggaacagg cttgatgctg ttcagacgat ggagcgtccg
     1921 aagcgagttg aaacgtccgt gcgagttgga gcggctcagg ccgggcttgt ggctggggtg
     1981 agtaaagcga attattaaaa atgaaaaagg actcgccgga agctgcctca gcgcgcggct
     2041 gcttgagctt gagagacgcg tgccccggtt tgcttctcta aaatcacgta gagcctgtag
     2101 gcatagaaga tgtctctgat ctgaaagtct tcgccagcga gccgctgcaa ctcggagagc
     2161 gcgggtgatg ggctggagcc gggtttcggt tcggggaggc tcaggatttt gtaacggcgg
     2221 gttgccgggg cggcctcgcg cgcgaagacg agcttctggc tgaccatctc ttcggactca
     2281 cattttatgt cgccccccat caatccctga tagctgtagc ccgccgcgat catcttgtcg
     2341 aaccccttca cgccttcgtc gtcctcgatg aaggtgtagt cgctgagtcc cgccgcacgt
     2401 cgctcgatca gcgcgacctt gaaggcgtct atgcgcccgc ctccgatgta gcgcgcccct
     2461 gctgtcgcga actggctcag ttccttcaac aggtttcttc tctctttgac cacctgatat
     2521 ttgatcttca ccggagccgc ctcgctcatg tccctctcaa gcacgactga gacgccggat
     2581 gaggcaagtc ccggtcctgc gaagaggagc cggacggggc ggaagcccgg cggtgcttcg
     2641 tcaatcccgg cctggatggt ctgctggagg ttctcgcccc agccgacccg tcccgtaagg
     2701 agcctgtact ccatcgtcgg ctccgcaacc ccgtcgcgcc gcatgaagag gaagacgctg
     2761 ctcttctccg tgctgagcaa cgtatccaag tcccatggtg gtttaggttc ggggctgtac
     2821 tccaccggag ggcacttgac cacggcgtaa gtctcaacga gtttgtagcc ccgccttgcg
     2881 ttgtagttga gcgggtggcc gtacgactct tcgaccggca tccggtcgtg ggcatatgcg
     2941 taggtgtggc ctgggtcgag gcgcagcaca gccgcgtact tccgccgctc gcccgtctcg
     3001 ccgtaactga ccgacttctc cacccggtag ccgagccgac cctgctcgtt caagtccctg
     3061 atgaagtcgt acaggccgcg cgagatgagt aacttcacct tctctaagtt cgtcgtgtcg
     3121 ataagcgtgg gtgcgggcgt cggcgcaggc gtgggcgcgg ggggtggcgg tttgcggcct
     3181 gactgcgcgg caacgcttga ttcaaaggcc agaagtgatg cggtgagtaa gaggactgcc
     3241 ttgagattct ggaacgtgaa catgctggcc tgacctttca tcgatgcaat acggcctcag
     3301 gactttcgca gtcaccggcg gcggcacgtc atttcagctg tgctccgccg cggcgtcagg
     3361 aagagaagag tcggcacgaa cgtcaacaac tacagggagc gcgacttcat gatctcctca
     3421 tggactagga tgctgcgccc tacctcaacg cgttgctcgc ccaagcacaa gggctgtgcc
     3481 cgagcctaca cgcggaatcc gtcaaatgcg tataccaaca ctgcgcccgg tgcggttcgc
     3541 ggtcgggttg catcccgcga ccggctccga tttctcgaac cccgctacca gcattaagct
     3601 gttacaaaaa atattttagc aaaaattact aatggctggc agcgggcacg tctcagaaaa
     3661 caaaattaaa gcagtctaag cgggttgcgg tgctcgcagt agtctgaact tgccttcaac
     3721 gcttccagcg ctgctgcgcc gtagataatc gcgggacagg ttgatctgtt cccgtccggg
     3781 cggcgacggg taggccgcgt ccggcgttga cgagcgctat gtccacgcca cagcactctg
     3841 actgcgggag gcttacccct tcagcggacc gccgcgcaag tctccagaac gaggccgcgc
     3901 agccagcgat gcgctggatc ggcatccagt cgcgggtgcc agagcaatga aaccgtgagt
     3961 tccggcgtgg agaacggaag gggaaaacta tgcattccgg cgcgcaggtt tcccgtgtgt
     4021 cgttcgggaa cactggcgat caggtcggaa gcgcgggcta gagccagcgc ggtcgaaaag
     4081 cctccgacaa tcgtgacgat cttccgttcc agcccgaacg gtttcaaggc ttcatcaatc
     4141 gccccctgat cgatcccccg ccgcgaaacg ctgatgtgcc ctccgcgcgc ataacgggcg
     4201 ggagtgatct tgcccttact cagtgtgtgg cctattcgca cgacgccaaa gaaacgatct
     4261 cggaacaacg tctgcgcccg cacttccgga cccatcgtgt tccccaccac gccagtctcc
     4321 agatcgacgg tgccgtcgcg tagagacgtg ctctctttgt ttggtttctg cacgaagcag
     4381 agccgcacgc cgggagcctc cacgccgacg cgagcaatga gttccggacc aaagttctcg
     4441 acaaagccct cgctggtacg cagcgtgaac gtgcggacga gttgtttgag gtcgggcgtc
     4501 tcggcaggac gtagaaccgc ttcaatgtcc tgcacaagct gaccgaccct ttcgcggagt
     4561 tcgagcgcgc ggggcgttgg aaccagaccg cgtcccgccc tgaccaacaa cggatcaccc
     4621 gtcgtctcac gcaagcgcgc aagcgcgcga ctcatcgccg atgggcttag cctcaaccgt
     4681 cgcgctgcgc gcgccacact cccttccgcc agcaccgcat caagagtgac aagcaagttg
     4741 aaatccgggg tcgacatgca tggattatag cgcagtttgt ttttgatgtg gcgtcaaacg
     4801 cacgaataaa gtgcaagtgg tgcgtcttcc gccatgccag ctaaagagct ataagtgaat
     4861 tcatgttgaa gacaatcatt gaagaacaaa atgaggcggg aaccggaagt gccaaactaa
     4921 caccttcggt tcgttgggcg cttgccagcc tttcactatc catgctgatg ccgtcgctcg
     4981 acacgagcat cgccaatgct ggcttgccga cgctggcaga agagttcgac gcttcctttc
     5041 aggaagtcca gtggatcgtc ctcgcctatc tactcgccat caccaccctg atcgtcagtg
     5101 ccggacggct cggcgacatc attggccgcc gacggctatt actgttcgga atcggtctgt
     5161 tcactacggc ctcgctcctg tgcggcgtcg cgcccacgct ccggctgctg atcgccgccc
     5221 gcgcggtaca gggtcttggg gcggcgatca tgatggctct cgctgtcgcc ttggtcagtg
     5281 agacggttcc gcaggcaaag accggaagcg ccatggggct gctcggaacg atgtccgcga
     5341 tcggcaccac gctcggtcca tcgctcggcg gccttttgat cgccggactc ggctggcgga
     5401 tgatcttcct cgtcaacgtg ccgctgagta tcctgaattt tcttctcgcg catcgctacc
     5461 tgcctgtcgc tcgtcgggcg gaaaagacgg agcgacgagc cttcgacaat gtaggcacgc
     5521 tgctgcttgg tgtgacgctt gcggcttatg cgctcgccat gacgatcggg cgcggccatt
     5581 tcggtcggct caacatcgcc ctgttgctgg ctgccgtctt cggagccggg tccttcgtcc
     5641 tcgtcgaggc gagagtcgca aagcctttgc tccggttggc gatgttccgc gatccggtgt
     5701 tgagtgcgag ccttgcgatg agcacgctcg tctcgactgt gatgatggcg acgctggtgg
     5761 tggggccttt ctatctctcg cgggggctgg ggcttgaaac ggcgcttgtc ggactcgctt
     5821 tgtcggctgg tccccttgtc gccgcgctga ccggcgtgcc ggctggtcgc cttgtagacc
     5881 gctttggcgc gcaacgaatg accatcaccg gactcgtcgg aatagcggtc ggctcttccg
     5941 ttctatccat gatgccgtcg agtttcggcg tcgttggcta catcgctgcc atagtcgtta
     6001 ttaccgcaag ctatgcgttg ttccaggcgg ccaacaacac cggcatcatg acaggtattc
     6061 gcccggatca gcggggcgtt atttccggcc tgcttagcct gtcgcgcaat ctcggactca
     6121 tcaccggggc atcggccatg ggcgcggtgt ttgcgctcgc atcggcgacg acggacatca
     6181 cgactgcgca tccagaggcc gttgccactg gcatgcggac cactttcgca gccgcggcga
     6241 ttctgctctt tgtcgcgctc accatctcgg ttggaagccg tgctctctcg cggcgtgcgg
     6301 tttctctaaa tcctgtatag gaggatggcc gaagcagagc cgttccgttg aaccatcaca
     6361 accattttta cggcagccgg agcttcctga atcgttattg actttagcga atcgtcacag
     6421 gcttaaaccc ggagcaggcg cggataaggc tcgggcaaat cttccttcat gaccaagttg
     6481 ccctcgatgc gtcgccccaa ctttttgaag gaatccttct tgcccgccaa gatggtaaaa
     6541 cccgtcggtt tcgtcatcag ttcgatgacc ttaagctggt cgggtttcgt taatgctctg
     6601 tacagatcaa tcaactcttt ctcttcaaac aagcccgtat cccacagaca caactcctgc
     6661 gcctcctctt cgacctgcat caagtcaacg agcttcattg tcttgagggt gttgaatcca
     6721 ttgatggcta cccaatcaac atcacaagtc ttaaacatcg ccatcgcctc gtcgaaacga
     6781 taagcattaa cgatgggatg caggaaggcg tcggcatcaa ttgacaactg gctccggtca
     6841 cgctccgcct gtgtcgctgt cgttccatat tgatcgggcg gcaatttgag cttgagctgc
     6901 tccatgatcc gttggccttt taaaaggtct gaacgttgag catcccagag cgtgagaagc
     6961 aactcgcgcc ccaccaggcg ttcaaactcg ccgaacggat ggtaatacca gagacagatc
     7021 actccatcgc ccgtcaggtg ctgacagaga ttaaatagtc ccttttgcgg gtcttcgagg
     7081 tgatgaacga cgcccgtaga aacaatgatg tcgaatgtct cctcaaggtc tagctccaaa
     7141 atatttctct gtttgaaagt gatattgtga atcccgtgct tatcagctaa ctgctcggcg
     7201 acttccagcg aggcttctgt caggtcaatt cccagaaact cagccttcgg gtattgcttg
     7261 gcgaacccta aaaccctctg acccgtccca cacccggcgt ccaggatctt ccggttatta
     7321 agagcatctt tcccgcaaag gaagtggaac aaattagcaa tgtcatacga taggctcgta
     7381 ccgacagtcg gactggggta agggtatttc gtatacatgg cgacgacatc ttctgttcta
     7441 acttttgaca taaagaatat tcctcgtgta gattttattt aattgatgct tgtccgccct
     7501 caaatgttgc ggcgcagtct gagatagttc gccccacatt ggcaggcgag actgcctctg
     7561 aaattaggca acaccgactt caaaaagggc atggaagatt gcgtgaggct aatggagcat
     7621 ttgtcagccg ggaactccga ccctttaaag cgcgtaagat attcctgtgc gggttagaat
     7681 gtcaatggtg agaatttaag ggcagtgatg tgatatggcc ggcacacctc cttgcagact
     7741 tgcttctact cactggcgaa aattactttc gatatcagac aacctgaaaa ggctaagagt
     7801 cttcctatat taaattgtca gcggcgcagc acacatggct actgcgtgcc ctcggtttcg
     7861 cgcccaaccg gctttagtaa tggcactggt ccgtgtttaa tctatgaaat tgaatagaca
     7921 tcaagttggc acatttagaa gttgacaaag cggcagatag gtatagtata ggtgttgcaa
     7981 acgatgacct gttaccccct tccccaaagg taaacgcttt aaggtcgatg ataaaataca
     8041 gtgagcagtg actcctgctt actatatttt tcttcacgca atatatttat aactggagcc
     8101 caatagctcg caaggtgtta gagagttaaa atggccgaac gcaagaaata aagccccatc
     8161 gctattttgc aggtagcgat agggcctaat tgacaacaca ctctgtcgca ttgtgcgaaa
     8221 agatgtatcg ccaacgtctc ttacatctta gcccacaatg cggcagagtc acaacctgaa
     8281 tttacaaatt ttcggagact ttgcccacat gcgcaatacc cttttctacc catctctaca
     8341 tttcatccct tttaaaaccc tcaggtccaa accgatcctg ggcaaaccat aacttcgctt
     8401 tgggagcccc ggacacgtgc tttggacggc atttgcctta tctggacctg caaaggtcta
     8461 tctagctgtg ggttatgaag tatgaacaat agaattgagc agggtgctgt tcctcgttgg
     8521 tatgtgattt acacccatcc aaaacaagaa aacagagccg acagtaattt gagagcttgg
     8581 ggagtggaaa ccttcaatcc gcaactgcaa gagacacgac gcaacatcta tactggcgca
     8641 ccagttaagg tcatctagaa tttattcccc agataccttt ttgcttagtt c
//
LOCUS       DASWNS010000633         8008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_75896, whole genome shotgun sequence.
ACCESSION   DASWNS010000633 DASWNS010000000
VERSION     DASWNS010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75896"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VGB76_21975"
     CDS             <1..664
                     /locus_tag="VGB76_21975"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY0101611.1"
                     /translation="YLPVDISASALDASARTLLQSYPALHIEAYASDYDTALARLAGE
                     RDTREEGRLLALFLGSNIGNFDRDGAEQFLRAMRGVLREDDALLVGADLKKDRATLEA
                     AYDDALGVTAAFNLNQLVRLNREFDADFKLRAFRHVAIYNEAAGRVEIYIESRLAQVV
                     ALPRLDMEITFAEGERIHTENSYKYDTDELDALAESTGFKCLRRWTDAAGRFSSNLFI
                     AR"
     gene            685..2160
                     /locus_tag="VGB76_21980"
     CDS             685..2160
                     /locus_tag="VGB76_21980"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEY0101612.1"
                     /translation="MTHDQLIEDAASRVRSSAPDERGGCALRDELAQAHGSNSRFPLL
                     TDAVDESSPSLFEENLRRGAKPATAWMCGIEFELFGYDARGHDGGDLARLTPAQVERV
                     LTGFAPSSGDLVYEGRTIVEVNAGQMNRLTVEPGGQIEFSGAPQRRLCDVECEVERYL
                     ARLHEIAEAEKFVFLAAGFDPLCTIEEQRWFPKMRYDVMRPYLAARGARAWDMMTRTC
                     AVQTNLDYGDESDLAKKFLVGNRLAPIATAIFANSPFEAGRPSGYKSTRAAVWLATDA
                     DRAGISPPALRENSFSPAGFVAYALEVPMLFAQRDGRYMNAPTGMKFGDFLARGCDTL
                     SPVFGDWADHLTTIFTDARLKQHLELRSADCGSLPHALAFQAYWKGLLYDPEALDAAL
                     RLAPNLDRADALALRAAVARDALAAPRTAGVDVLALAKESIALAVEGLTRIAPDELSY
                     LDMLREQVVADELSPADILLRNWHGSWHASTPRLLEHLRIA"
     gene            2182..2580
                     /locus_tag="VGB76_21985"
     CDS             2182..2580
                     /locus_tag="VGB76_21985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101613.1"
                     /translation="MQTEHADQSFFLAARLVDNSQHDDAITVLRPLLECELDAMVKSM
                     VCINMAIIHGLKGQDSDALIWYDRGIGYERSLGRFFVAERKAAFMAEKNWNADSLTMY
                     ERLLNEPSLTGEDRERIEQNISLLKERLGY"
     gene            complement(2591..4063)
                     /locus_tag="VGB76_21990"
     CDS             complement(2591..4063)
                     /locus_tag="VGB76_21990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0101614.1"
                     /translation="MSVAKELLKTGAPRLTRTDDASSCERVVNDVHSQLNETRVRRIF
                     APDSMNALQALVRRARKAGRAVSVAGGRHAMGGQQFAAGGLLVDTRRMNRVLAFDPSR
                     REIEVEAGIEWPELINFMFEAQRGRAPVEQAAIVQKQTGADRLSIGGALAANIHGRGL
                     NLRPFVDDVESFVLVDAEGEARTCSRLENRELFRLAIGGYGLFGVVASVRLRLARRRK
                     LERVAEISATRDLADSFDKRIADDFLYGDFQFITDEASPDFLRRGIFSCYRPVADDAP
                     MPEEQKELCAGEWQHLYHLAHVDRRRAFEVYSKHYLATSGQLYWSDTHQLSVYLDDYH
                     RQLDLAMKASVRATEMITEVYVPRAALADFLEVVRRDFLRHAVELIYGTVRLIKRDRE
                     SFLAWAKEDYACVVFNLHTEHSATGIAKAREDFRRLITRAIHMRGNYYLTYHRWATRR
                     QVELCYPQLPDFLRLKRQYDPAETFQSDWYRHYRKMFAAG"
     gene            complement(4063..5004)
                     /locus_tag="VGB76_21995"
     CDS             complement(4063..5004)
                     /locus_tag="VGB76_21995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0101615.1"
                     /translation="MKAGNPSATAYLIATSTLFMAADPDAGRLIPPRAVELSASFVAA
                     RTRLARRAQRELRRKSVRPFVSALERLTLPGIQLHYALRKLYLEETARAALSASGGIR
                     QVVVFGAGYDTLALRLHETFPSAQFIEIDHPDTQRRKTRALAAHLDSRLQRPQTPNLH
                     FLPFDLTRPQLAATLLNFAAYRTAAPTLFIAEGLLMYLSPLEVSRLLQTIRECSGARS
                     RFAFTFMEPQAHGRVNFRTRSRAVDAWLSWRGELFKWGIERDALPVYLRERGFNLREI
                     ATDETFRRRYLEPRRLAHLPLATGEYVCLADLEGRDV"
     gene            complement(5208..5609)
                     /locus_tag="VGB76_22000"
     CDS             complement(5208..5609)
                     /locus_tag="VGB76_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0101616.1"
                     /translation="MRKLFLATIIIVATFSVAAAQDAAASSSNQSDIPNMGRAPSEPN
                     GIGRLDARVFDEAGNPVPGAHLKLESERTDGYFCESWNSSDARGVSVLPPLHMGNLKL
                     IVKAKGYETQKINVTAATLNEPFRITLVRKK"
     gene            5784..6062
                     /locus_tag="VGB76_22005"
     CDS             5784..6062
                     /locus_tag="VGB76_22005"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0101617.1"
                     /translation="MTWIDLLVLLLVAGICGALGQAISGFSRGGCLVSIALGFIGAVL
                     GMWLASQLGLPELFNVSVGGRNFPVVWSIIGSALFVAVISLLTRGRRE"
     gene            6156..7949
                     /locus_tag="VGB76_22010"
     CDS             6156..7949
                     /locus_tag="VGB76_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101618.1"
                     /translation="MAFASIKTTTPASATNSTSTPDLKYFRQHYPLTVANFGALGDRE
                     AHLRRIWQTEMRWQETLDEQRRGAPEAVVHGAPPPGLAVEGEFEIIYAGGTLGLLHAA
                     VLAARFKRPVMVFDAGVFGRDDASSSSSSRRAWNISGEELGGFERAGLFTKEEIEAAI
                     VNRYRSAFVKFHDAASRVKTPPLWMEDVLNVAVDADKLLALAAAKVRARQGEGCALLE
                     HVRFVRAYVEADRVLVELEDVRAGGNSGGNAGGRRRLFAARLFIDAEGMQSPVARQFN
                     EGRALTHVAPSVGTVARGFARGEGADRVDFNVGEILVSTEDARDHRQLIWEGFAGARA
                     RDEYATRLFFYDTIDSPADKSLLALFERYFEELPRYKRAGAQWRVERPLYGYAPGANP
                     RGWKARAQTAEDRVMLVGEATGASSPLTLSGFGSSVRRLRRLTHLTDLALAADLLDAR
                     ALSEINAPAPRVAQMSGFAEFLRPAPQSEPANVNETLNAVMAALHDLDVRVRHELFQD
                     RMSFSALKSLLSRTAKLYPRIFQRVREHLGARGTFWWLAGIAEAAFSERRARVEAGGQ
                     LSSGESAHAPAEEFARYVALYKKDREADASD"
BASE COUNT         1477 a         2513 c         2523 g         1495 t
ORIGIN      
        1 ctatcttccg gttgacatct ccgcctccgc cctcgacgcc tccgcacgca cgctcctgca
       61 atcttatccc gcgcttcaca tagaggctta cgcgagcgat tacgacaccg cgctcgcacg
      121 actcgcgggc gagcgcgaca cgcgcgaaga ggggcgtctg ctcgcgctct tcctcggctc
      181 gaacatcggc aacttcgacc gcgacggcgc ggaacaattc ttacgcgcca tgcgcggcgt
      241 gctccgcgag gatgacgcgc tcctcgtcgg cgcagacttg aaaaaagatc gtgccactct
      301 ggaagcggct tacgacgacg cgctcggcgt caccgccgcc ttcaatctca atcaactcgt
      361 gcggctcaac cgcgagttcg acgccgattt caaactgcgc gcctttcgcc acgtcgccat
      421 ctacaacgaa gcggcgggtc gcgtggaaat ctacatcgag agcaggctcg cgcaagtcgt
      481 cgcgctccca cgtctcgaca tggagatcac cttcgccgag ggcgagcgca tccacacgga
      541 gaactcttac aagtacgaca cggacgaact cgacgctctg gccgaaagca caggcttcaa
      601 gtgcctccgc cgctggactg acgcggcggg acgttttagc agcaacctct ttatcgcacg
      661 ctgaaagatt cgggcgcacc gcgtatgact cacgaccaac tgattgaaga tgccgcgagc
      721 cgcgttcgca gcagcgcgcc ggatgagaga ggcgggtgcg ccctgcgcga tgaactggcg
      781 caggctcacg gcagcaatag tcgcttcccg cttcttaccg acgcggttga cgaatcttct
      841 ccgtcgctct tcgaggaaaa tttacggcgc ggcgcgaagc ctgcgacagc gtggatgtgc
      901 ggcatcgagt tcgaactctt cggctacgat gcgcgcgggc acgacggcgg cgatctcgcg
      961 cgcctgactc cggcgcaggt cgagcgcgtg ctcacgggct tcgcgccttc ctccggtgat
     1021 ctcgtctatg aggggcgcac catcgtcgaa gtgaacgcag ggcagatgaa tcgcctgacg
     1081 gttgagccgg gcgggcagat cgagttctcc ggcgcgccgc agagaaggct ctgcgatgtc
     1141 gagtgcgaag tcgagcgtta cctcgcgcgt ttgcatgaga tcgccgaagc ggaaaagttc
     1201 gtcttcctcg ccgccggttt cgatccgctg tgcacgattg aagagcagcg ttggtttccc
     1261 aagatgcgct acgacgtgat gcgcccctat cttgcggcgc gcggcgcacg cgcctgggac
     1321 atgatgacgc gcacctgcgc cgtgcagacc aatctcgact acggcgacga aagcgatctc
     1381 gccaaaaagt ttctcgtcgg caatcgcctc gcgcccatcg cgacggccat cttcgccaac
     1441 tcgccctttg aagcggggcg accctccggt tacaaatcca cgcgcgccgc cgtctggctc
     1501 gcaaccgacg ccgaccgcgc agggatttcg ccgcccgcgc tgcgcgaaaa ctctttctcg
     1561 cccgccgggt tcgtcgccta cgcgctcgaa gtgcccatgc tcttcgcgca gcgcgacggg
     1621 cgttacatga acgcgccgac cggcatgaag ttcggcgact tcctcgcacg cggatgcgat
     1681 acgctctcgc ccgtcttcgg cgattgggca gaccacctca ccaccatctt caccgacgcg
     1741 cgcctcaagc agcatctcga actacgcagc gcagactgcg gcagcctccc gcacgcgctc
     1801 gcctttcaag cctactggaa aggattgctc tacgacccgg aggctttaga cgctgcgctc
     1861 cgcctcgcgc cgaatctcga ccgcgccgac gcgctcgcac tgcgcgccgc cgtcgcacgc
     1921 gacgcactgg ctgcgccgcg cacggcggga gttgacgtgc tcgcgctcgc caaagaatct
     1981 atcgcgctcg ccgtcgaagg actcacacgc atcgcgccgg atgaattgtc ctacctcgac
     2041 atgctccgcg agcaagtcgt cgcagacgaa ctctctcccg cagacatcct gctgcgcaac
     2101 tggcacggct catggcacgc ctccacgcca cgccttctcg aacacctccg catcgcctga
     2161 aaacgtagga gcgaattgct tatgcaaacc gaacacgccg atcagtcctt ctttttagcc
     2221 gcgcgtctcg tggacaactc ccagcacgac gacgccatca ccgttctgcg tcccctgctc
     2281 gaatgcgaac tcgatgccat ggtgaagtca atggtctgca tcaacatggc gatcattcac
     2341 ggcctgaaag gacaggactc ggacgcgctc atctggtacg accggggcat cggctacgaa
     2401 cgctccctcg gacgcttctt cgtcgccgaa cgcaaggccg ccttcatggc cgagaagaac
     2461 tggaacgccg acagcctcac catgtacgaa agactgttaa acgaaccctc cctcacgggc
     2521 gaagaccggg aacgcatcga gcagaacatc tccctcttga aagaacgcct cggctattaa
     2581 gcccgcgaag ttaaccggcg gcaaacatct tccggtagtg gcgataccag tcgctctgga
     2641 aagtttcggc ggggtcgtat tggcgtttga gtcgtaggaa gtcggggagt tgcgggtagc
     2701 agagttcgac ttgcctgcgc gtcgcccagc gatgataggt gagatagtag ttgccgcgca
     2761 tgtggatggc gcgggtgatg agacggcgaa aatcttctcg cgctttagcg atccctgttg
     2821 cgctgtgctc cgtgtgcagg ttgaagacga cgcaggcgta atcctccttc gcccacgcga
     2881 gaaaactctc acggtcgcgc ttgatgagcc gcaccgtccc gtagatcaac tcgaccgcgt
     2941 ggcgaagaaa atcgcggcgc acgacttcaa gaaaatctgc gagcgcggcg cgcggcacgt
     3001 aaacttccgt gatcatttcg gtcgcgcgca cgctcgcttt catcgccaga tcgagttgac
     3061 gatggtagtc gtctaaatag acgctgagtt ggtgcgtgtc cgaccagtag agttggccgg
     3121 acgtagcgag gtagtgtttg gaatagactt cgaaggcgcg gcggcgatca acgtgcgcga
     3181 ggtgatagag atgctgccac tcccccgcgc agagttcctt ttgttcttcc ggcatcgggg
     3241 catcgtctgc gacggggcga tagcaggaaa agatccctct gcgtaaaaaa tcgggcgagg
     3301 cttcgtcggt gatgaactga aaatcgccgt agagaaaatc gtctgcgatg cgtttatcga
     3361 atgagtccgc caagtcgcgc gtggcggaga tctccgcgac gcgttccagt ttgcgccgcc
     3421 gcgccagacg caggcgcacg ctcgcaacca cgccgaacag accatagccg ccgattgcca
     3481 ggcgaaacaa ctcccggttc tcaaggcggc tgcacgtccg cgcctctccc tccgcatcca
     3541 cgagcacgaa cgattcgaca tcgtccacga agggtcgcag attcaaaccg cgcccgtgga
     3601 tgttggcggc gagcgcgccg ccgatgctca gtctgtccgc gccggtctgc ttctgcacga
     3661 tggcggcctg ctcgacgggt gcgcgcccgc gctgcgcctc gaacatgaag ttgatcaact
     3721 ccggccactc gatccccgct tcaacttcga tctcgcggcg cgacggatca aaggcgagca
     3781 cgcgattcat gcgccgcgta tccacgagca gaccgcctgc ggcgaactgc tgcccgccca
     3841 tcgcgtgcct gccgcccgcg actgaaacgg cgcgtccagc ctttcgcgca cgccggacca
     3901 acgcctgcaa agcgttcatc gaatcgggtg cgaagatgcg acgaacgcgc gtctcgttca
     3961 gttgcgaatg tacgtcgttg acgactcgtt cgcacgaact cgcgtcgtcc gtacgcgtca
     4021 ggcgcggcgc gcccgttttc aacagttcct ttgcgacgct catcaaacgt ctctcccttc
     4081 gaggtctgcg aggcagacgt attcgcctgt ggcgaggggc aggtgcgcga ggcgtcgggg
     4141 ttcgagatag cgtcggcgaa aagtttcgtc ggtcgcgatc tcgcgcaggt tgaaaccccg
     4201 ctcgcgcagg tagacgggca gcgcgtctcg ctcgatgccc catttgaaga gttcgccgcg
     4261 ccaactgagc cacgcatcta cggcgcggct gcgcgtgcga aaattaacgc gcccgtgcgc
     4321 ctgcggctcc atgaacgtga aagcgaagcg gctccgcgcg ccgctacact cgcggatcgt
     4381 ttgcaagaga cggctcacct ccaacggcga gaggtacatc aacaagccct cggcgataaa
     4441 gagcgtaggc gcagcggtgc gataagcggc gaagttaaga agcgtcgcgg cgagttgtgg
     4501 ccgcgtgaga tcgaagggga ggaaatgcag gttgggcgtc tgcggacgtt gcaggcgcga
     4561 atcgagatgc gcggcgagcg cgcgggtctt gcgtcgctgc gtgtctgggt ggtctatctc
     4621 gataaactgc gcggagggga acgtttcgtg gagacgcaag gccagcgtgt cgtagcccgc
     4681 gccgaagacg acgacctgac ggatgccgcc ggatgcggag agcgcggcgc gcgcagtctc
     4741 ttcgagatag agtttgcgga gcgcgtagtg cagttgaatg ccggggagcg tcagacgctc
     4801 aagcgcggag acgaaagggc gcacactctt gcgtcgcagt tcacgttgcg cccggcgcgc
     4861 gaggcgcgtg cgcgccgcga cgaaggacgc gctgagttcg acggcgcgcg gcgggatgag
     4921 acgcccggcg tccgggtctg cggccataaa gagcgtgctc gtcgcgatga gatatgcggt
     4981 tgcgctgggg ttcccggctt tcatctgccc tcctctacgg aaggccgagc atagcttcag
     5041 ccgccgccgc gcttgcgccc ggctgtggca ctttatgaat tgtcatagag gcgcgccgcg
     5101 cgcctccgca ccaaaataga gaaggagcat gcggcgaagt tcaggacttc accgcatgct
     5161 cctgatcgag cgtcgaataa gtttttgcgc gtgcgcgccc gcgcgtccta tttcttcctg
     5221 acgagcgtga tgcggaacgg ttcgttgagg gtcgcggcag tgacgttgat cttctgcgtc
     5281 tcgtagcctt tggctttgac gatcaacttg aggttgccca tgtgcagcgg cggcagcacg
     5341 gagacgccgc gcgcgtccga ggaattccac gactcgcaga agtagccgtc cgtgcgctcg
     5401 gattcgagtt tgaggtgtgc gccgggcacg gggtttccgg cttcgtcgaa gacgcgcgcg
     5461 tcgagtcgtc cgatgccgtt cggctcgctg ggcgccctgc ccatgttggg gatgtcgctc
     5521 tggttggaag acgaagcggc ggcatcctgc gcggcggcaa cgctgaaagt ggcgacgatg
     5581 atgatggtag cgagaaacaa tttacgcatc tctgataaaa tctcctgaac tttaggtgca
     5641 aaatttgatg agggtcgagg gcgtgagcgc gagcaacaac ttctcccggc cacgaacgat
     5701 tatattgaca aacgcgcaaa ttggaaagaa gaaactgcgc gaacggctcc tgcgtttaac
     5761 ttgaaacaag aggtgacaag gttatgacat ggattgatct gctcgttttg ctgctcgtcg
     5821 ccggaatctg cggcgcgctc ggacaggcga ttagcggttt ttcacgcggc ggctgtctcg
     5881 tctcaatcgc gctcggtttc atcggcgcgg ttctcggcat gtggctggca agccagttgg
     5941 ggctgccgga attattcaac gtgtctgtgg gcgggcgcaa tttcccggtc gtctggtcga
     6001 tcatcggctc cgcgctcttc gtcgccgtca tcagcctgct cacgcgcggg cggcgcgagt
     6061 gaaaagttca tagtcattta gtgattgatt tgtaaccggc gcgcgctaga gtcacgcctc
     6121 tcctaactca gatttccggg atttccgttt ttcgtatggc tttcgcttca atcaagacaa
     6181 ctacgcccgc gtcggctact aactctacga gcacgcccga cctcaaatat tttcgccagc
     6241 actacccgct cacggtggct aacttcggcg cgctcggcga ccgcgaggcg cacctgcgac
     6301 gcatctggca gacagagatg cgctggcagg agactctgga cgagcagcgg cgcggcgcgc
     6361 cggaagcggt cgtgcacggc gcgcctccgc ccggcctcgc cgtcgaaggc gagtttgaga
     6421 tcatctacgc gggcggcacg ctcggactcc tgcacgcggc ggtgctggcc gcgcgcttca
     6481 aacgtcccgt gatggttttc gacgcgggcg ttttcgggcg cgacgacgcg agcagcagca
     6541 gcagtagccg ccgcgcgtgg aatatttccg gcgaggaact cggcgggttt gagcgcgccg
     6601 gtctgtttac gaaagaggag attgaagcgg caatcgttaa tcgttatcgt tcggccttcg
     6661 tcaagtttca cgatgcggct tcgcgcgtga agacgccgcc gctctggatg gaggacgtgc
     6721 tcaacgtggc cgtcgatgcg gacaagttgt tggcgttggc cgccgctaaa gttcgcgcgc
     6781 ggcagggcga aggctgcgca ctgcttgagc acgtgcgttt cgtgcgcgcc tacgtggaag
     6841 cggatcgcgt cctggtggag ttggaggacg tgcgcgctgg cggcaatagc ggcggcaatg
     6901 cgggcgggcg caggcgtctg ttcgcggcgc ggcttttcat tgacgcggaa ggcatgcagt
     6961 cgcccgtcgc gcggcagttc aacgaggggc gcgctttaac gcacgttgcc ccgtcggtcg
     7021 gcacggtggc gcgcgggttt gcgcgcggcg agggagccga ccgggttgat ttcaacgtcg
     7081 gcgagatttt ggtgagcacg gaggacgcgc gcgaccatcg ccaactgatc tgggaaggct
     7141 tcgcgggcgc acgggcgcgc gacgaatatg cgacccgcct ctttttttac gacaccatcg
     7201 attcgcccgc cgataagtct ctgctcgcac tcttcgagcg ttactttgaa gaattgccgc
     7261 gctacaaacg cgcgggcgcg cagtggcgcg tcgaacgccc gctgtatggc tatgcgccgg
     7321 gcgcgaaccc acgcggttgg aaagcgcgtg cgcaaacggc ggaagatcgc gtgatgctcg
     7381 tcggcgaggc gacgggcgcg agcagcccgc tcaccttatc cggcttcggc tcatccgtgc
     7441 gccgcctgcg acggctcacg catctcaccg atctcgcgct cgccgccgac ctgctcgacg
     7501 cacgcgcgct ctccgagatc aacgcgcccg ccccgcgtgt ggcgcagatg tccggcttcg
     7561 cggaattttt gcgccccgcg ccgcagagcg aaccggccaa cgttaacgag actctgaacg
     7621 ccgtgatggc cgccctccac gatctggacg tgcgtgtgcg tcacgaactt tttcaagatc
     7681 gcatgtcgtt cagcgcgctc aagagtctcc tgagccgcac ggccaaactc tacccgcgca
     7741 tcttccagcg cgtgcgcgaa catctcggcg cacgcggcac gttctggtgg ctcgcaggga
     7801 ttgccgaagc cgctttcagc gaacggcgtg cgcgcgtgga ggctggcggc caactcagta
     7861 gcggcgaaag cgcgcacgcg cccgccgaag agtttgcccg ctacgtcgct ctctacaaaa
     7921 aagatcgaga ggcggacgcg tcagactaaa gcgattcggt tgtggttatt attgtcgcgc
     7981 gcgagcgaag cgatccgaaa aaaatgat
//
LOCUS       DASWNS010000634         8107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_440957, whole genome shotgun sequence.
ACCESSION   DASWNS010000634 DASWNS010000000
VERSION     DASWNS010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440957"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VGB76_22015"
     CDS             <1..261
                     /locus_tag="VGB76_22015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101619.1"
                     /translation="NAFKVAVSNYSQLKGADFNDPESLQSVGQSAFQTEILIDQLDVD
                     RDGTGEVFTISYSFEGTNYAIYKKGRAGWTRLKEFHNYRCAY"
     gene            396..1364
                     /gene="fmt"
                     /locus_tag="VGB76_22020"
     CDS             396..1364
                     /gene="fmt"
                     /locus_tag="VGB76_22020"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY0101620.1"
                     /translation="MRLVFMGTPETAVPTLKRCLDDGHEVVAVWTQPDRAAGRGYSLR
                     LPPVKEFALAHGLTVHQPAKIKTEEARELFASHEADACIVVAYGRILPSAFLSAPRRG
                     CLNVHFSLLPKYRGAAPVNWAIVQGETETGVTTMRIDEGLDTGAIYLQRATSIGADET
                     APQLLARLAPLGAQLLSETLARLDELEPRPQREADATHAPMLRREDGLVDWRAQAAEI
                     ERRVRGFQPWPNAHTQFRGQRLVMHQAALGSVAADETGRSDAAFGELTKAHGDELEVA
                     CGERTRLALREVQLEGKRRMSARDFINGMRVCAGERLGDESIGDRG"
     gene            1471..2904
                     /gene="rsmB"
                     /locus_tag="VGB76_22025"
     CDS             1471..2904
                     /gene="rsmB"
                     /locus_tag="VGB76_22025"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEY0101621.1"
                     /translation="MAIRVVKKGRAGQGAANEAGGVRRGVSASRRAAFEVLRRVEDEG
                     AFASVLLASATTELRADDRALCYELVLGCLRRQLWLDKLIEHYAKRDAGRLDAPVRRA
                     LRLGLYQLRFLTRIPPSAAVNESVNLAYEARLRSAANFINAVLRRATREPDYDPAAQA
                     TNELERLSIAASHPRWLVERWTKFFGAGEVESFAHANNETPPVAFRVNPLGANREEVL
                     ERLRGAGVAVQPSRIAPHAWRVEGAGGGSGGAASPALLRALAGEGAIYMQDEASQLVA
                     HLVGARACERVLDVCAAPGSKTTQLAALSNDEAFVVAGDIYAHRLRTLGESAARQNLK
                     NVRLIAYDAEKWPLPFAAGAFERVLVDAPCTGTGTLRHNPEIRWRITQADIAGLAARQ
                     RRILAHAAQTVCSGGRLVYSTCSVEPEENEEVVSAFLETHPGFKLIRPDVPEQLLTKE
                     GAARTWPQRDGTEGFFVAVFERQSGKG"
     gene            3095..3907
                     /locus_tag="VGB76_22030"
     CDS             3095..3907
                     /locus_tag="VGB76_22030"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEY0101622.1"
                     /translation="MAGTQRPLGSALGKVAILIAIAGLFLVGMAGTVYLSLRSPEVQV
                     PDVTGKNIVDAEQILSDAGLNVRRRASRYSAEVSADTVLDQTPRAGDLVKGGQTIAVV
                     ISRAEAKEGESSVGVRRDEEAKKDNQNSPENKNGEPASAAAANKNENANENQNRPKRN
                     KNTNKNTNNANANSNASGNSNVNSNLNANLTTNSRNAENRNAAPRPDAGRNTNARPNS
                     NNPDPVNRNTGATPNANAPVNRNAGANANRSRTTNNRNANAAPARNANNPRP"
     gene            4152..4814
                     /gene="rpe"
                     /locus_tag="VGB76_22035"
     CDS             4152..4814
                     /gene="rpe"
                     /locus_tag="VGB76_22035"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY0101623.1"
                     /translation="MIEIAPSILSADFARLGEEIAAIERGGATLVHCDVMDGHFVPNI
                     TIGLPVVKSLRRATRLPLDTHLMISEPGRYAEQFVEAGADMVSVHVEIDPHLNRTLVG
                     IRSKGAQAGIAINPATPLVALEESLAFADYVLLMTVNPGFGGQKFIPTMLDKVRRLRR
                     MIEERGLKTRIEVDGGIDRTNIADITAAGAEIIVAGTAVFGGGDPAQGVRELLDSAAQ
                     WV"
     gene            4943..6592
                     /gene="bamD"
                     /locus_tag="VGB76_22040"
     CDS             4943..6592
                     /gene="bamD"
                     /locus_tag="VGB76_22040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY0101624.1"
                     /translation="MRLRRFLLVAGACTIFVSGLPVASNARAQEQPRAQSEGTAVQRL
                     EVLRQRLDTSRRTLNGAIAGLNAGDDKQKKDQKAAVDDPAARLRGLEKEVSSLLNEVN
                     ETRSRAERGAEKFEVKDVDKLESAFADLNGRVESGLQATAGARRTATVATNATGSKNK
                     KKPGFFGRILGRGGDDDEYAELVGGVAPGRDRQLFEVAAKEARRSKYEEARYLFNVII
                     TTYPDSPYLPHAKLAIADTFYLEGSTSALIQAANAYQEWLTFFPTDPLADEVMLKVAE
                     VEMRQMGLPDRDVAHARKAEQRLKALLQQFPNTALRPDAQIRLSEVQENLGMHTFQVG
                     NFYMDRYERGVAPNPKGAQSRYREVIEKYPHFSRRDRALYLLANTYVLEEEPDEAAKY
                     FQEIVRNFPNSEFAEKAAEQLEAIGAAKPEADPKAKDKLPRVKASMTSRFLQQVFGTN
                     SVTVDKNGVLINKDSNESSLIDVIIDNKGTLPVTTPNAPTINRRVPAVSRPSAQAQPA
                     PAAPVTTDKATDGVKLTPTNPGAPRTVDTPSAPPTQPPPAN"
     gene            6829..7350
                     /locus_tag="VGB76_22045"
     CDS             6829..7350
                     /locus_tag="VGB76_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101625.1"
                     /translation="MAEKIEAELTRRHRAASMLVSALITLTLTLVALAYAGARPRVNL
                     SFIDQTAYVALWIVILFAGLGAIALRRMRFGALRLQTIAEAKGLSALLATLHKTTLLV
                     ALFSVVIAVIGYVLFMRSTDPWDMAKAGIIAIAILLYTYPRRAAWQRVLQATQTKDGL
                     TTDGVPPVKGTPA"
     gene            7347..>8107
                     /locus_tag="VGB76_22050"
     CDS             7347..>8107
                     /locus_tag="VGB76_22050"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101626.1"
                     /translation="MKFSVHLASLAFAVSACAFACPVPGYAQAEPLSPVAQPTATPAR
                     PQAQPTAQTSVQPTLNARERRVKAYAKLMEGQRFFTSARGAGGITREDLNSAQKAFEL
                     AAALDPTLSEAHTALAEIAFFFLDDQTLAERHATAAVRVDNNNFGAQRILSRIYSLKV
                     GLDPDKMDRAAADRAIAALKEVLRIDPGDGEALALIGEFYAATGRDNEAIDAFRRWMG
                     APATVDTNFFKVISQGRELSSDAAAARLAQAYLKAG"
BASE COUNT         1644 a         2486 c         2523 g         1454 t
ORIGIN      
        1 aacgccttca aggtcgccgt ctcgaactac agccagttga agggcgcaga cttcaacgac
       61 ccggagtcgc ttcaaagcgt cgggcagagc gcgttccaga cggagatact cattgatcaa
      121 ctcgacgtgg acagggacgg cacgggcgag gttttcacca tcagctacag cttcgagggc
      181 acaaactacg cgatctacaa aaaagggcgc gcgggctgga cgcgcctcaa agagtttcac
      241 aattacaggt gcgcttactg aaaacgttga aggagaaaac aggagacaga attcaggaaa
      301 cagaataagc acgcgggtct gacttctgac ttctgtgttc tgaattctgt ctcctgtttt
      361 ctgttttctc cttcatcgtt acttttacta aaagcatgcg tttggttttc atgggcacgc
      421 cggagacggc tgtgccgacg ctcaagcgtt gtctcgatga cgggcacgag gtcgtcgccg
      481 tgtggacgca gccggacagg gctgcggggc gcggctacag tctgcgcctg ccgcccgtca
      541 aagaatttgc tttggcgcac ggcctcacgg tgcatcaacc cgcgaaaatt aagacggaag
      601 aagcgcgcga gttgtttgcc tcgcacgagg cggacgcgtg catcgtggtc gcttacgggc
      661 gcatcctgcc gtccgcgttt ctcagtgcgc cgcgtcgcgg gtgtctgaac gtccatttct
      721 ctctgttgcc gaagtacagg ggcgcggctc cggtcaactg ggctatcgtg caaggcgaga
      781 cggagacggg cgtgacgacg atgcggattg acgagggact cgacaccggg gcgatctacc
      841 tgcaacgcgc cacttcgatt ggtgcggacg aaaccgcgcc gcaactcctc gcgcggctcg
      901 ccccgctcgg cgcgcagctt ttgagcgaga cgctcgcacg cctcgacgaa ctcgaaccgc
      961 gcccgcaacg cgaagccgat gccacgcacg cgccgatgct gcgcagggaa gacggcctgg
     1021 ttgattggag agcgcaggcg gcggagatcg aacggcgcgt gcgcggcttt cagccgtggc
     1081 cgaacgcgca cacacaattt cgcgggcagc gactcgtcat gcatcaagcc gcgttgggaa
     1141 gcgtcgcggc ggatgagacg gggcgcagcg acgccgcgtt tggtgaactg acgaaggcgc
     1201 acggcgatga gcttgaagtc gcgtgcggcg agcgcacgcg tctggcgtta cgtgaagttc
     1261 aacttgaagg caagcggcgc atgagcgcgc gcgactttat caacggcatg cgcgtgtgcg
     1321 cgggcgagcg gctgggggat gaaagcatag gggacagagg atagggtaga gcggaaagta
     1381 gaagggtaag ggataaagca gaggcagctt caagttgact ctttgttttc tcctttaccc
     1441 tttccccctt ccgctttacc ctaaacctgt atggcgattc gtgtggtcaa aaaaggcagg
     1501 gcggggcagg gcgcggcgaa tgaagcgggc ggcgtgcggc gcggcgtgtc ggcgtcgcgg
     1561 cgcgcggcgt ttgaagtctt gcgccgggtg gaggatgaag gggcgttcgc gtcggtgctg
     1621 ctggcgagcg cgacgacgga gttgcgcgcc gatgatcgtg cgctctgtta cgaactcgta
     1681 ctcggctgtt tgcgtcgcca actctggctc gacaagttga tcgaacacta cgcgaagcgc
     1741 gacgctggac gcctcgatgc tccggtgcgt cgcgcgctgc gtctggggct gtaccagttg
     1801 cgctttctca cgcgcattcc gccgtcggcg gcggtcaacg aatcggtcaa cctcgcctac
     1861 gaagcgcgcc tgcgttcggc tgcaaacttc atcaacgccg tgctgcgccg cgccacgcgc
     1921 gagccggatt atgatcccgc cgcgcaagcg acgaacgaac tcgaacgcct ctcaatcgcc
     1981 gcttcacatc cacgctggct cgtcgaacgc tggacgaaat tcttcggcgc gggcgaagtc
     2041 gaaagtttcg cgcatgctaa caatgagacg ccgcccgtcg cgtttcgcgt caacccgctt
     2101 ggcgcaaacc gcgaggaggt tttggagcgt ctgcgcgggg cgggcgtggc cgtgcagcct
     2161 tcgcgcatcg cgccccatgc gtggcgcgtg gagggcgcgg gcgggggcag tggcggcgca
     2221 gcctctccgg cgttgctgcg cgcgttggcg ggcgaaggcg cgatctacat gcaggatgag
     2281 gcgtcgcaat tggtggcgca tctcgtcggc gcgcgtgcgt gtgaacgcgt gctcgacgtg
     2341 tgcgccgcgc ccggcagcaa gacgacgcaa cttgccgccc tgtcgaacga cgaagcgttc
     2401 gtcgtcgcgg gcgacatcta tgcacatcgc ctgcggacgc tcggcgagtc ggcggcgcgg
     2461 cagaacttga aaaacgtccg gttgatcgcc tatgacgcgg agaagtggcc gctcccgttt
     2521 gctgcgggcg cgtttgagcg cgtgctcgtt gacgcgccgt gcaccgggac gggcacgctc
     2581 aggcacaacc ccgaaattcg ctggcgcatc acacaggcgg acatcgccgg actcgccgcg
     2641 cggcagcgac gcatcctcgc acacgccgcg cagacggtct gctccggcgg tcgtctcgtc
     2701 tattcgacct gctcggtcga accggaagag aacgaagagg tcgtctcggc atttctggag
     2761 acgcatccgg gtttcaagtt gatccggcca gacgtgccgg agcaactctt gacgaaagaa
     2821 ggcgcggcgc gcacctggcc gcaacgcgac ggcacggagg gtttcttcgt cgcggtgttt
     2881 gaaagacaat cgggaaaagg gtagtagggg gaaggggtaa aggtaaaagc aaaaggtaaa
     2941 agcaaagggc agctttaagt tgattcttcg tcctctcgct tgccgttttc tcctctccgt
     3001 ccggcctttt cgtttcgccc gcgccgatgt tagactctcg cgtttgcaaa acgcccaatt
     3061 tcgcgggcgc gtcttgatga ggtaattaaa gaagttggca ggaacacagc gtccgctcgg
     3121 atcggctctc ggcaaggttg ctatcctgat agccattgcc ggactcttcc tcgtcggcat
     3181 ggcgggcacg gtctacctct ctctgcgcag ccccgaagtg caggtgcccg acgtgacggg
     3241 caagaacatc gtggacgccg agcagattct ctccgatgcg ggattgaacg tgcgccgtcg
     3301 cgcctcgcgc tacagcgcgg aagtcagcgc cgacaccgta ctcgatcaga cgccgcgcgc
     3361 gggcgatctc gttaaaggcg ggcagaccat cgccgtcgtc atcagtcgcg ccgaagcgaa
     3421 agagggcgaa agttccgtcg gcgtcaggcg cgacgaagag gcgaaaaagg ataaccagaa
     3481 cagtcccgaa aacaagaacg gcgagcctgc ttctgctgcc gccgccaaca agaacgagaa
     3541 cgccaacgaa aatcagaacc gcccgaaacg caacaagaac acgaacaaga acacgaacaa
     3601 cgcgaacgcc aactcaaacg cgagcggcaa ctccaacgtc aactcgaatt tgaacgccaa
     3661 cttgacgacc aacagccgca acgccgagaa tcgcaacgct gcgccacgcc ccgacgccgg
     3721 acgcaacacg aacgcgcgac cgaacagcaa taatcccgac cccgtgaacc gcaacacggg
     3781 cgcgacgccg aacgcgaacg cccccgtcaa tcgcaacgcg ggcgcaaacg cgaaccgcag
     3841 tagaaccact aacaaccgta atgcgaacgc ggctccggcg cgtaacgcca ataacccgcg
     3901 cccctgagct tttgcggacg cgaagccgga aacgcaccgt cgtctggcga agcgtagata
     3961 gttttgcgcg gggcttaaat tcgagtctgc gttggtctta aacttgaaac actaactccg
     4021 gcacgagcag ttttcatccg atttaatcca agtttcatta agttttattc aatattcggc
     4081 ttttcgacgg ttgacaggcg acggcggcgc ggatccgcgc cgggaatctt gtcggatcgg
     4141 gcaaggcaac gatgatagag atcgccccat ctattttatc tgctgatttt gcacggcttg
     4201 gcgaggagat cgcggccatc gagcgcggcg gcgcgaccct ggtgcattgc gacgtgatgg
     4261 acggccattt cgtgccgaat atcacgattg gcctgcccgt cgtaaagtct ctgcggcgcg
     4321 caacccgtct gccgctcgac acgcatctaa tgatttccga gccggggcgt tacgcggagc
     4381 agtttgtcga agcgggagcc gatatggtgt cggtgcacgt cgaaattgac ccgcatctga
     4441 accgcacgct cgtcggcatc cgcagcaaag gcgcgcaggc cggaattgcc atcaacccgg
     4501 cgacgccgct cgtcgcgctc gaagaatcgc tcgcgttcgc cgactatgtt ctcttgatga
     4561 ccgtcaatcc cggtttcggc ggccagaaat tcatcccgac catgctcgat aaagtgcgtc
     4621 gcctgcggcg catgatcgag gagcgcggac tcaagacgcg catcgaagta gacggcggca
     4681 tcgaccgcac gaatattgca gacatcaccg ccgccggagc cgagatcatc gtcgccggaa
     4741 cggcggtctt cggcggcggc gacccggcgc aaggcgtccg cgaactgctg gactccgccg
     4801 cgcaatgggt ttgaaaagca gtgacgagtg acaagtgacg ggtgacgagt taaagcaaag
     4861 aacttgtctt ccaacttctc ttatttcttg ctcgtcactt gtcaccatag tcactagtca
     4921 ctgttcttgg aggttttatt aaatgcgttt acgcagattt ttattggtcg caggcgcgtg
     4981 cacgatcttc gtgagcggtt tgccggtcgc gtcgaatgcg cgggcgcagg agcagccgcg
     5041 cgcgcagagc gaggggacgg cggtgcaacg cctcgaagtg ttgcggcaac gtctggacac
     5101 ttcgaggcgc acgctcaacg gcgcaatcgc gggactcaac gccggcgacg ataagcagaa
     5161 gaaagatcaa aaggctgcgg tcgatgatcc ggcggcgcgg ctgcgcgggc tggaaaaaga
     5221 ggtgagttcg ctcttaaacg aagtgaatga gacgcgttcg cgtgccgagc gcggcgccga
     5281 aaagtttgaa gtcaaggacg tggataaact cgaaagcgcg ttcgccgacc tcaacgggcg
     5341 cgtcgagtcc ggcttgcagg cgacggcggg cgcacgccgc accgcgaccg tcgccaccaa
     5401 tgcgacgggc agcaagaata agaagaagcc cggtttcttc gggcgtattc tgggacgcgg
     5461 cggcgatgat gacgagtatg ccgaactcgt cggcggagtt gcgcccggac gcgaccgcca
     5521 gttgtttgaa gtcgcggcga aagaggcgcg ccgttcgaaa tacgaggaag cgcgttatct
     5581 cttcaacgtg atcatcacca cctatcccga ttcgccttac ctgccgcacg cgaaactcgc
     5641 catcgccgac accttctatc tcgaaggttc gacgagcgcg ctcattcaag cggccaacgc
     5701 ctaccaggag tggttgacgt ttttcccgac cgacccgctg gccgatgaag tgatgttgaa
     5761 agtcgcagag gtggagatgc ggcagatggg cttgcccgac cgcgacgtgg cgcacgcgcg
     5821 caaggcggag caacgtctga aagctctctt gcagcagttc ccgaatacgg ccttgcgccc
     5881 cgacgcgcag attcgcttga gcgaggtgca ggaaaatctc ggcatgcaca cttttcaggt
     5941 cggcaacttt tatatggatc gctacgagcg cggcgtcgcc ccgaatccga agggcgcgca
     6001 gtcgcgttat cgtgaagtga tcgaaaagta tccgcacttc tcgcgccgcg accgcgccct
     6061 ctatctctta gccaacactt acgtgctgga ggaagagcct gacgaagcgg cgaagtattt
     6121 tcaagagatc gtgcgcaatt tcccgaacag cgaattcgcc gaaaaggccg ccgagcaact
     6181 cgaagccatc ggcgcggcca agccggaagc cgacccgaag gcgaaggaca aactgccgcg
     6241 cgtgaaagcc tctatgactt cgaggttcct gcagcaggtg ttcggcacca acagcgtcac
     6301 ggtggataag aacggcgtgc tcatcaacaa ggacagcaac gagagcagcc tgatcgatgt
     6361 catcatcgac aacaaaggca cgctgcccgt taccacgccg aacgcgccca cgatcaaccg
     6421 tcgcgtcccc gcggtttcgc gaccctccgc acaggcgcaa cccgcgcccg ccgcgccggt
     6481 cacgaccgac aaggccaccg acggcgttaa actcacgccg accaatcccg gcgcgcccag
     6541 aaccgtcgat actccgagcg cgccgccgac ccagccaccg cccgcgaatt gaagccacgc
     6601 gggaagggca caggaaacaa ggtcaagggg caggggtaga gggaaaaggt attaaccctt
     6661 tacccctgcc cctttatcct ttagccattt tcgttcccct tcctgcgttt tactttcgag
     6721 tcggcggtgt tagaattttt gcgcctccca aacgtgtgaa ataggatcgt ttagagcgcg
     6781 tcgagcggcg tgcgcgacgg ttgcggcggg gaacccgaac ggactgatat ggccgaaaag
     6841 attgaagctg aattaacgcg ccgccatcgt gccgcctcga tgctcgtctc ggcgctcatc
     6901 acgctcacgc tcacgctcgt cgcgctggcc tacgcgggcg cgcgtccgcg cgtcaacctg
     6961 agtttcatcg accagacggc ctatgtcgcg ctctggatcg tgattctctt cgccggactc
     7021 ggcgcaatcg cgctccggcg catgcgtttc ggcgcgttgc gcctgcaaac catcgccgag
     7081 gccaaaggcc tctccgccct gctcgccacc ttgcacaaga ctaccttgct cgtcgctctg
     7141 ttcagcgtcg tcatcgccgt catcggctac gtgctgttca tgcgttcgac cgatccgtgg
     7201 gatatggcga aggcgggaat tatcgccatc gctattctgc tctacactta cccgcgccgc
     7261 gcggcctggc agcgcgtcct gcaagcgacg cagacgaagg atggcttgac caccgacggc
     7321 gtgccgccag tgaaaggaac acctgcgtga agttttctgt ccacctcgcc tcgctcgcct
     7381 tcgcagtttc ggcgtgcgct tttgcctgtc ccgttcccgg ctacgcgcag gcggaaccgc
     7441 tcagccccgt ggcgcaaccc acggccacgc ccgcacgccc gcaggcacag ccaaccgcgc
     7501 agacttccgt acagcccacg ctcaacgcgc gtgagcgacg cgtgaaggct tatgcgaagt
     7561 tgatggaagg gcagcgtttc ttcacgagcg cgcgaggcgc gggcggcatc acgcgcgagg
     7621 atttgaattc ggcgcaaaag gctttcgaac tggccgccgc gctcgaccct actctttcgg
     7681 aagcgcacac cgcgctcgcc gagatcgctt tcttcttcct cgacgaccag acgctcgccg
     7741 aacgacatgc gacggctgcc gtccgagtcg ataacaacaa cttcggcgcg cagcgcatcc
     7801 tctcgcgcat ctattcgctc aaggtcggac tcgacccgga caagatggac cgcgccgccg
     7861 ccgaccgcgc tatcgcagcc ctgaaagagg tcttgcgtat tgatccgggc gacggcgaag
     7921 cgttggcttt gatcggcgag ttctacgcgg cgacggggcg cgacaacgaa gcgattgatg
     7981 cgttcaggcg ttggatgggc gcgcccgcca ccgtcgatac caattttttc aaagtcatct
     8041 cgcaggggcg cgaactctcg tccgatgctg ccgccgcgcg gctcgctcag gcctatttga
     8101 aggccgg
//
LOCUS       DASWNS010000635         3677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_128285, whole genome shotgun sequence.
ACCESSION   DASWNS010000635 DASWNS010000000
VERSION     DASWNS010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128285"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2147
                     /locus_tag="VGB76_22055"
     CDS             <1..2147
                     /locus_tag="VGB76_22055"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEY0101627.1"
                     /translation="GGGVTVSGSGNFIGGAGAGEGNLISGSNFASVVIGGMQNVVKGN
                     LIGTQADGISPLGNNGAGVVCPHNSSGNVIGGTESGAGNVIAYNAGSGIADTSTDSMT
                     PTIRGNTFRGNSIFSNNALGIDVGDDSRVTLNDAGDGDQNRQNFPLITSATTGAGGIN
                     LKGTFNSAANATFALDFYANEVCDATGFGEGKVFIGTANLTTDALGNADINVSLPVAV
                     APGKFITVTATNANSETSEFSQCRAARENVENDPCVYTLSPANKTSPAAGETFTVGVL
                     TQNGCHWTATSNSNFISVTSGANSSGNGLLTLVVSRNESGVPRTGTVTIGGQTFTVTQ
                     PEIIPGAPAIQFSERFYQAYENTERVTLTVLRSGDLSNAASVDYRTVDVDTFTTGCAN
                     GGQGAFGRCDFSATYGRLAFSPGELHKTITIPLINDGHSEATEPFQVVLSNPVGLILG
                     GTSTAQVHVLDDDPLGTPNPIFNTPFFVRQHYLDFLSREPEPSEPWSAVLDRCPNVNR
                     DPVCDRITVSQSFFGSTEFRLKGSYVFRFYKVAFDRLPHYTEIVADMSFVAGATEAEV
                     YARKAQLATLFTERFEFQTAYTRLSQSAFVAALLGRYGITQITTPDPAQPDGSAKVTL
                     TQSELITKLDTNALTRAQVLRAVADSEQVSQAEFNNTFVAMQYYGYLRRTPEESGYQA
                     WLRVIKQDPNNIRIMVDGFMNSTEYRLRFGQP"
     gene            complement(2433..2957)
                     /locus_tag="VGB76_22060"
     CDS             complement(2433..2957)
                     /locus_tag="VGB76_22060"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0101628.1"
                     /translation="MQNSNLYIRYARIEDASLLAELGAQTFAETFTDDNSPADMAAYL
                     ADSFSLEKLKTELTDPLSIFFIAEVDAHAAGYAKLHSGKALESVEGQNPIELVRLYVS
                     RKWLGRRVGQALMQRCIDEARGKGFQTIWLGVWERNSRALAFYRKWNFREVGEHIFQL
                     GSDPQRDILMQRAI"
     gene            complement(3163..3236)
                     /locus_tag="VGB76_22065"
     tRNA            complement(3163..3236)
                     /locus_tag="VGB76_22065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3200..3202),aa:Arg,seq:tcg)
     gene            complement(3285..>3677)
                     /locus_tag="VGB76_22070"
     CDS             complement(3285..>3677)
                     /locus_tag="VGB76_22070"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0101629.1"
                     /translation="TVSNGNSNNSNAASTPANVTANASAPAASDELASARTTYQAVCA
                     RCHRDNGEGGTVEFEEGGALKVPSFKTGHGLTHTEAQFARQIAKGGDGMPAFEKRLTP
                     EQINGLVRYIRQEIQAGLLKDGAPNPSH"
BASE COUNT          789 a         1061 c         1040 g          787 t
ORIGIN      
        1 atgggggcgg ggtgacggtc agcgggagcg gtaatttcat cggcggcgcg ggggcgggcg
       61 agggcaatct catctccggc agtaactttg cgtccgtcgt aatcggcggc atgcaaaacg
      121 tggtgaaggg taatttgatc ggcacacagg ccgacggcat cagcccttta ggtaacaacg
      181 gggcgggggt ggtctgcccg cacaattcaa gcggtaatgt catcggcggg acggagagcg
      241 gagcgggcaa cgtcatcgcc tataatgccg ggtcgggcat cgccgacact tcgaccgact
      301 ccatgacgcc gacaattcgg ggcaacacct ttcgcggaaa ctccatcttc tcgaacaatg
      361 cgctgggcat cgatgtcggc gacgatagcc gcgtcacact gaacgacgcg ggcgacggcg
      421 atcaaaatcg ccagaacttc cccctcatca cttccgccac gacgggcgca ggcggcatca
      481 acctcaaggg caccttcaac agcgcggcca atgctacgtt cgcgctcgat ttctatgcca
      541 acgaggtttg cgacgccacg ggcttcggcg agggcaaagt ttttatcggc accgccaacc
      601 tgaccaccga cgcgctcggc aatgccgaca tcaacgtctc tctacccgtc gccgtcgcgc
      661 ccggcaagtt catcaccgtc acggccacga acgccaacag cgagacttcg gaattttccc
      721 aatgccgcgc cgccagggag aatgtcgaga acgacccgtg cgtctacacg ctcagccccg
      781 cgaataaaac ttcaccggcg gcgggcgaga ctttcacggt gggcgtgctg acgcagaacg
      841 gttgtcattg gactgcgacc agcaatagca acttcatcag cgtcacgtcc ggcgcgaaca
      901 gttccggcaa cggcctgttg acgctcgtag tctcgcgaaa cgaatcgggc gtgccgcgca
      961 cgggcaccgt gaccatcggc gggcagacct tcaccgtcac ccagccggag atcatccccg
     1021 gcgcgcccgc cattcagttc tccgagcgtt tctatcaagc ctatgaaaac acggagcgcg
     1081 tcacgctaac cgtcttgaga agcggcgact tgtcgaacgc cgcctcggtc gattaccgca
     1141 ccgtcgatgt agatactttt acgaccggat gcgccaatgg cgggcagggc gcgtttggcc
     1201 gttgcgactt ctccgcgacc tatggccggc tcgccttctc ccccggagaa ctgcacaaaa
     1261 cgatcacgat cccgcttatt aacgacggcc actctgaagc tacggaaccc tttcaggtgg
     1321 tgctgtcgaa tccggtcggc ctcattctgg gcggaacctc gacggctcag gtgcacgtgc
     1381 tcgacgacga tccgttgggg actccaaacc caatcttcaa cacgcccttc ttcgtccgcc
     1441 agcattatct ggacttcctc tcgcgcgagc cggaaccgtc cgagccgtgg agcgcggtct
     1501 tggaccgctg cccgaatgtt aaccgtgatc ctgtctgcga ccgtatcacc gtctcgcagt
     1561 cgttcttcgg ctcgaccgag ttccgcctga aaggctccta cgtgttccgc ttctacaagg
     1621 tggccttcga ccgcctgccg cactacacgg agatcgtcgc ggacatgagc ttcgtcgccg
     1681 gagcgacgga ggcggaggtg tacgcacgca aggcgcaact ggcaactttg ttcacagagc
     1741 gttttgagtt ccagacggcc tacacacgtc tctcgcagag cgctttcgtc gccgcgctgc
     1801 tcggcaggta cggcatcact cagatcacga cgccagaccc ggcgcagccc gacgggagcg
     1861 cgaaggtgac tttgacgcag tccgagttga tcacgaagct cgacacaaac gcgctgacac
     1921 gggcgcaagt gttgcgcgcc gtggccgatt ccgaacaagt atcgcaagcg gagttcaaca
     1981 acaccttcgt ggcgatgcag tattatggat atttacggcg aacgcccgag gagtcgggct
     2041 atcaggcatg gctcagagtt attaaacaag acccgaacaa catccgcatc atggttgacg
     2101 gttttatgaa ctccaccgag tatcgcctga ggttcggcca gccataaaga ggctgcacag
     2161 agaaaaccgg tagggcttga accggcgacc ttttgattcg aactcaccgt actatcttta
     2221 tcaccggaga agggtctttg ggtagagggg gttagaactt taacgataag ttctgcatcg
     2281 cctatgtcgg cgccagacac ttacggtgct gtggacgcgc cgaattactt cattaaccgg
     2341 gcggcaattg cctgaacaat aaatcaagcc tcacgcatga aagcaatgct gttgtcgctc
     2401 tgtttgaatg agcgattatg cttcgccacc ggttaaatag ctcgctgcat caaaatatca
     2461 cgttgcgggt cagagccaag ttggaagata tgctctccga cttcgcggaa attccacttc
     2521 cgataaaatg ccagggcgcg gctgttgcgt tcccatactc caagccagat agtctgaaat
     2581 ccctttccgc gcgcttcgtc aatacaccgc tgcattaacg cttgaccgac tctgcgccca
     2641 agccattttc gggagacata caatcgcacc aattcaatcg ggttctgacc ttcgacgctc
     2701 tccaatgctt tgcccgagtg aagctttgcg tagcccgctg catgagcgtc aacctcggca
     2761 atgaaaaaga tagataaagg gtcggtcaat tccgttttca gcttttcgag gctaaatgag
     2821 tcggctaaat atgccgccat atccgcaggc gagttatcgt cggtgaatgt ttcggcaaat
     2881 gtttgcgccc ccaattctgc cagcaaactg gcatcttcga tgcgggcgta acgaatgtat
     2941 agattagagt tttgcatcaa tatcctttac ggctcaatta tacaagaaga agcatcactg
     3001 catttagacg taccaatcgt cgtgtcagct aacccctcta tcaggcttct aagtaaatta
     3061 ttccaataga aaatacaact atgtaggagg ggcgcgggtg tgattgtgta cgacaacgaa
     3121 ataggcacta ttagtgtacg tctaaatcat tgtatttatt gatacacccg gtagggctcg
     3181 aacctacgac cttttgattc gaagtcaaac gctctatcca actgagctac gggtgcgtgt
     3241 tgtctgcaat gggcgggaga ataacatagg cgcgtgcgtt ttgattagtg ggaagggttg
     3301 ggtgcgccgt ctttgagtaa gcccgcctgg atttcctgtc ggatgtagcg gacgaggccg
     3361 ttgatctgtt cgggcgtgag cctcttttcg aaggcgggca tgccgtcgcc gcctttggcg
     3421 atctggcgcg caaactgcgc ttcggtgtgc gtcaggccgt ggccggtttt gaaactgggc
     3481 actttgagtg cgccgccttc ttcgaactcg actgtgccgc cttcgccgtt atctcgatgg
     3541 cagcgggcgc agaccgcctg ataggttgtg cgagccgagg ccaattcgtc ggaggccgca
     3601 ggagcggatg cgttggcggt cacgttggcc ggggtcgaag ctgcgttcga gttgtttgaa
     3661 ttgccgttag agacggt
//
LOCUS       DASWNS010000636         4826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_386915, whole genome shotgun sequence.
ACCESSION   DASWNS010000636 DASWNS010000000
VERSION     DASWNS010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386915"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..690
                     /locus_tag="VGB76_22075"
     CDS             118..690
                     /locus_tag="VGB76_22075"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY0101630.1"
                     /translation="MPKIDSIEETPNPNAVKFVLKEPVTTGAARSFLSPTLAEDDALA
                     RSLFEVGGVVSVFYMDRMITVEKSETEDWDDLLPKLAVPIRAAEAAQGGAVAAAAAGA
                     GALGGTLGALMSDDPRLLRINEILDQKVRPALAGDGGWLEIVSLKENTLTIKYQGACG
                     SCPSSISGTLMAIESMVQQEVDPALEVVAV"
     gene            1079..1261
                     /locus_tag="VGB76_22080"
     CDS             1079..1261
                     /locus_tag="VGB76_22080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101631.1"
                     /translation="MTRFDEPLRQWANLTVTQDRAKELVDLITRREDSESLDALLELI
                     CGLMPYPPCAAWEYRV"
     gene            1386..3320
                     /locus_tag="VGB76_22085"
     CDS             1386..3320
                     /locus_tag="VGB76_22085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101632.1"
                     /translation="MSSRLRLCAVALCLPTLLPAPHTHAQTSTAAAQPKKPAAATAKK
                     TGTLDPLAAERRANAITLLNTLADESRGFRDQTLRARVQVQAADALWETDKERARALF
                     RRAWDAAELADAENTRRAEEQNRASGRAGTRGLNLPNLCGEVLRLAARRERALGEEFL
                     GRMEEARKRETTELNNASASLPAAGTGGAANAGGQPTRRDPDAVPPDVARRLRLAMQF
                     LEDSDIERALAFADPVLNNVNPNSVEFLVLLREKSAQEADRRFNALLAGTAADPNSDA
                     GDILLLSSYVLTPHLYMTIGQGGQGVSSSQRRREIATPDLPAALRVTFARAAAQVLLR
                     PLPQEDQDATATGRVGTFFVISRLLPFFEQVIPEGVPALRARLAALNPDIPEQARKGL
                     GEDLTRGLVPEDSRDGVQESLDAAARATDPRDRDTAYFRAAQEAAFKGDLRARDYADK
                     IGDADLRRQVRGFVDFSLLNRAITDKKDGMEVLRLATAGELTPVQRTWAYTEAARLLA
                     KDDRPRALEALEAAATSARSIDAEDPDRVRSTVAVATRFFEFDRNRAWEIMADAVKAA
                     NAAPEFTGSDGGLAARIRTRNQSSTINFPAPVFDLTGVFQTLAKDDMNRAIALAQTFT
                     GESPRAVATLAIAGAVLGKK"
     gene            complement(3540..4082)
                     /locus_tag="VGB76_22090"
     CDS             complement(3540..4082)
                     /locus_tag="VGB76_22090"
                     /inference="COORDINATES: protein motif:HMM:NF025160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEY0101633.1"
                     /translation="MTSDEREVTSGSLTGARMRAEREARDDATRAAWPAAWAWVFPAT
                     YALHILEEWWGGFNVWAARLTGVEATPGEFLLWNGVALLSMSASIVLTLRFKSFGWLL
                     LAYGTAFLLNALSHLIASLYTTSYSPGLVTGLLFWLPLGALVLLRFRKRLTRRARRAG
                     LLVGLLIHCVVLLVTLHGGR"
     gene            complement(4111..>4826)
                     /gene="bshB1"
                     /locus_tag="VGB76_22095"
     CDS             complement(4111..>4826)
                     /gene="bshB1"
                     /locus_tag="VGB76_22095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEY0101634.1"
                     /translation="AVFSHPDDAELTVAGTLLKLKSLNYRTGVLDMTRGEMGTRGTVE
                     GREAEAREAARIMQLDARLNLEQPDGHLMLTDEARRALVRVFRALRPRVVFTSHWDDP
                     HPDHAATARLVREAARLASMRRYDEEHGLAVVPMPSLAHAVYSRLVVPSFVVDVSDFV
                     EEKMRAIRAHVSQFYREGTQEPETRISDKNFLAQMEYRMRYYGSLINVAAGEAFYVRE
                     ALNIEDPVALLTRPMNIYS"
BASE COUNT          930 a         1562 c         1464 g          870 t
ORIGIN      
        1 gaagtataat ctgccctgcg attaaaattt cgacgcctgt tttttagcgg tgcgccccat
       61 gcttgcggcg catctaaatt tatactggat gaagcaattt tatttggaga atcgagaatg
      121 cctaaaattg atagtatcga agagacgccg aacccgaacg cggtcaagtt tgtcctgaaa
      181 gaacctgtga cgacgggcgc ggcgcgctcc tttcttagcc cgacgctggc ggaggacgac
      241 gcgctcgcac gttccctgtt cgaagtgggc ggagtcgtct ccgtctttta catggaccgt
      301 atgatcactg tggagaaatc ggagacggaa gattgggacg acctgctgcc gaaactggcc
      361 gtccccatcc gcgcggctga ggcggcgcag ggcggcgcgg tagcggcggc ggcagccgga
      421 gcgggcgcac tcggcggcac gctcggcgcg ctgatgagcg acgacccgcg cctcctgcgc
      481 atcaacgaaa tcctcgacca gaaggttcgc cccgcgctcg caggcgacgg cggctggctc
      541 gaaatcgtca gtctcaaaga gaacacgctg acgatcaagt atcagggcgc gtgcggcagt
      601 tgcccgagtt ccatctccgg cacgctgatg gcaatcgaga gcatggtgca acaggaagtt
      661 gaccccgctt tggaagtcgt cgccgtttaa ccggaggcac gtcagacaaa attggagggc
      721 agcgaacaca ttcactgccc tccaatttta tgcctcggct tgagctgcct aagccgactt
      781 tttcgctgct cgcttttgcc cgccactttt cttactatcc tctgcatccg tctctgcttt
      841 aacctttggc ggtctgccga cttttcgatt ttcaactaac tttaaatctt cctcatctac
      901 aacatatgtt cgcccgaact gttgcacggg tagccgctgt gaactaataa gttcctgcac
      961 gcgtctcacg ctgacaccta atctctctgc ggcttgcgct gtcgttatca atcccatgtc
     1021 gttatttttt acagacgaag aaactattcg gcgatgcgcg cccttttttg ttgacttaat
     1081 gacgcgtttc gacgaaccgc tccggcaatg ggcgaacctg acggttacac aagacagggc
     1141 gaaagagtta gttgatctga tcacaaggcg cgaggatagt gagagtctgg acgccttgct
     1201 ggagttgatc tgtgggctga tgccttatcc tccgtgtgcc gcgtgggagt atcgcgtgtg
     1261 agtcgccggg tcaacttttt acatctttaa catcattttt ctgaactgag ggcgggaacg
     1321 ggcgtataag ttgacgcagc aatttcatcc gaggcgcgtt ctgcgccgcc gtcaggagta
     1381 aaactatgag ttctcgtcta cgcttgtgcg cggtcgcgct ttgcctgccg actcttttgc
     1441 ccgcgccgca cacacacgcg caaacctcta ccgccgccgc tcaacccaag aaacccgccg
     1501 ccgcaaccgc gaagaaaacg ggcacgctcg acccgctcgc cgccgaacga cgcgcgaacg
     1561 ccatcacgct cctcaacacg ctcgcagacg aatcacgcgg tttccgcgac cagacgttgc
     1621 gcgcgcgcgt gcaggtgcag gcggcagacg cgctctggga gacggacaag gaacgcgcac
     1681 gcgctctctt ccggcgcgcg tgggacgctg cggaactggc cgacgcggag aatacgcgtc
     1741 gcgctgaaga gcagaaccgc gccagcggtc gcgccggcac gaggggtctt aatcttccga
     1801 acctgtgcgg cgaagtgttg cgcctcgcgg ccagacgcga acgcgcgctc ggcgaagaat
     1861 ttctcggtag gatggaggaa gcgcgcaaac gcgagacgac cgaactcaac aacgcctccg
     1921 ccagcctgcc tgcggcgggc acgggcggcg cggcgaacgc tggcggccag ccgaccagac
     1981 gcgaccccga tgccgtcccg cccgatgtgg cgcggcggct gcgactcgcc atgcagtttc
     2041 tcgaagacag cgacatcgag cgtgcgctgg cgttcgccga tccggttctc aacaacgtca
     2101 acccgaactc ggtcgagttt ctagtcctct tgcgcgagaa gagcgcgcag gaggccgacc
     2161 gccgtttcaa cgcgctgctc gcaggcacgg cggctgatcc gaactccgac gcgggcgaca
     2221 tcctcctgct ctcctcttac gtgctcacgc cgcatctcta catgacaata ggacagggcg
     2281 ggcagggcgt ctcttcgagc cagcgtcgca gggagatcgc cacgcccgac ctgcccgccg
     2341 ccttgcgcgt caccttcgca cgcgccgccg cgcaggtgct cctgcgcccg ctcccgcagg
     2401 aagatcagga cgcaaccgcg acgggtcggg tcggcacgtt cttcgtcatc tcgcgcctgc
     2461 tgccgttctt cgaacaggtc atccccgaag gcgtcccggc cttgcgtgcg cgcctcgccg
     2521 cgctcaaccc ggacatcccg gagcaggcgc gtaaaggtct gggcgaagat ttgacgcgcg
     2581 gcctcgtgcc cgaagattcg cgcgacggcg tacaggaatc gctcgacgcc gccgcacgcg
     2641 ccaccgaccc gcgcgaccgt gacacggcct actttcgcgc cgcgcaggaa gcggccttca
     2701 aaggcgacct gcgcgcgcgc gactacgcag ataagatcgg agacgcggac ttgcgaaggc
     2761 aggtgcgcgg cttcgtagac ttctccctgc tcaatcgcgc catcacggat aagaaggatg
     2821 gaatggaagt tttgcgtctc gccacggcgg gcgagttgac tccggtgcag cgcacttggg
     2881 cttacacgga ggccgcgcgc ctgctcgcca aagacgaccg cccgcgcgca ctcgaagcgt
     2941 tggaagcagc ggccacttcc gccaggagca tcgacgcgga agaccccgac cgcgtgcgct
     3001 cgaccgtcgc cgtcgccacg cgcttcttcg agttcgaccg caaccgcgcg tgggagatca
     3061 tggccgacgc ggtgaaggcc gcgaacgccg cgcccgaatt cacaggctcc gacggcggcc
     3121 tcgccgcgcg catccggacg cgcaaccaaa gctcgacgat caacttcccc gcccccgtct
     3181 tcgatctcac gggcgtcttc cagacgctcg ctaaagacga tatgaaccgc gccatcgcgc
     3241 tcgcgcagac gttcacgggc gagtcgccgc gcgccgtcgc cacgctcgcc atcgcaggcg
     3301 cggtgctcgg caagaagtga cgagtgatga gtgacgggtg acgagataaa aatttgcatc
     3361 tcgccacttg tcgccggggc gggttgccat tgcagaggaa ttaaaaactt caccgccggg
     3421 acacagagga cgcgaaaggg gcacagaaaa ttttatcttc tctgtgccct cttcgtgtct
     3481 gcgccggtga agttttgcgg cgttgaagtt ttttggttgc tctcaattgc gaatcgtgat
     3541 caacgcccgc cgtgcaacgt cacaaggagc acgacgcagt gaatcagcag tccgacgagc
     3601 agacccgcgc gacgtgcgcg ccgcgtgagt ctcttcctga aacgaagcaa cacgagcgcg
     3661 ccgagtggca gccagaagag cagacccgta acgagtccgg gcgagtatga agttgtgtag
     3721 agactggcga tgaggtgcga gagcgcgttg agcagaaagg ccgtgccgta ggcaagcagc
     3781 agccagccga aagatttgaa acgaagcgtg aggacgatgc tggcagacat cgacagcagc
     3841 gcgacgccgt tccataacag aaactctccc ggcgtcgctt cgacgcccgt aagccgcgcc
     3901 gcccacacgt tgaagccgcc ccaccactct tccagaatgt gaagcgcgta ggttgcggga
     3961 aagacccaag cccacgccgc aggccacgcc gcgcgcgtcg cgtcgtcgcg cgcctcacgc
     4021 tccgcacgca tccgcgcccc cgtcaaactc ccgctcgtca cttcccgctc gtcactcgtc
     4081 acttgtcact cgtcactgcc ttcccgtcgc tcaggaataa atattcatcg ggcgcgtgag
     4141 gagcgcgacg gggtcttcga tgttgagcgc ttcgcggacg tagaaggctt ctccggcggc
     4201 gacgttgatg agcgagccgt agtagcgcat gcggtattcc atctgcgcga gaaaattctt
     4261 gtcgctgatg cgcgtctccg gttcctgtgt gccctcacgg taaaactgcg agacgtgcgc
     4321 gcggatcgca cgcatcttct cttcgacgaa atccgacaca tcgaccacga atgacggcac
     4381 gacgagccgc gaatagaccg cgtgcgccag cgacggcatc ggcacgacag ccagcccgtg
     4441 ctcctcgtca tagcggcgca tgctggcgag ccgagcggct tcgcgcacga gccgcgccgt
     4501 cgccgcgtga tcgggatgcg ggtcgtccca gtgtgaagtg aagacgacgc gcgggcgcaa
     4561 ggcgcgaaag acgcgcacga gcgcgcggcg cgcttcgtcc gtgagcatca agtggccgtc
     4621 gggctgttcg agattgagcc gcgcgtcgag ttgcatgatg cgcgccgcct cgcgcgcctc
     4681 cgcttcgcgc ccttcgaccg tgccgcgcgt gcccatctcg ccgcgcgtca tgtcgagcac
     4741 gcccgtccgg taattcaagg atttcaactt caagagtgtg cccgccaccg tcaactccgc
     4801 atcgtccggg tgcgagaaga cggcca
//
LOCUS       DASWNS010000637         4707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_334373, whole genome shotgun sequence.
ACCESSION   DASWNS010000637 DASWNS010000000
VERSION     DASWNS010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334373"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VGB76_22100"
     CDS             <1..653
                     /locus_tag="VGB76_22100"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0101635.1"
                     /translation="LPRRTDDAEGLLARIGNGHLVVMLDRLDEWQIAYVILKGSYRQI
                     REAGIDALRQSLVSIAPELADRVAHLKEWKQVSMLSVESSRVAQWYQPGLLLIGDAAH
                     VMSPVGGVGINYAIQDAVVAANTLAEPLRDGRLELRHLRIVQRQREWPTRFIQRIQAF
                     IQQVMIANILRSNQPLQVPRIVRLFLRTPFLRDIPARIIAFGLRRVHVKPSLRPPA"
     gene            complement(807..2258)
                     /locus_tag="VGB76_22105"
     CDS             complement(807..2258)
                     /locus_tag="VGB76_22105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0101636.1"
                     /translation="MSGRYENRVQLVLALVVGLLLVANLFTLTLVAMTPPDRNVRPPV
                     LFAVFTITLLISLPGIFLLPRWLVRPYRQLVGEAERAPVAARPEGARDETEFVLETFQ
                     AVVAQLRAQQRELEKLSAQASARAASAEMFNERVVASVPSGLVAFDAGGLATVINPPA
                     RALLEIESNSTGQHLKTLLRLSPELTNLVERCLLSGELYRREEVAATTGEGRVRRLGV
                     TVAPIDPQAGSGARGALCLLTDITEVAQLREALALKRNLESLGEMSAGLAHEFKNALA
                     TLHGYAQFLQNLELDERGRGAASALLQEVRNLTQMITAFLNFARPQPLQLSEVSMREL
                     LTDCADELQPFYAERAVSFDLSGEFPSVRADELMLRQALLNLLRNAAEAFEETQTRAQ
                     RRVSAHGEVETGDSGRKQLVISIEDTGTGISQSELQRIFIPFFTTKSKGHGIGLALAH
                     RVVTEHGGTLSAANAPAGGAVFTLRLPLENPGG"
     gene            complement(2346..3227)
                     /locus_tag="VGB76_22110"
     CDS             complement(2346..3227)
                     /locus_tag="VGB76_22110"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY0101637.1"
                     /translation="MTQLQNLTSFADSTLMPFPLALILVSTFGALIGSFLNVVIHRVP
                     REESIAFPNSRCPSCGTPIRPYDNVPILSYLVLRGRCRACGVSISARYPAIEALTALL
                     FALVFLHDGLTLALPFDLIFVAALVALIFIDAEHMILPDVITLPGLLFALIVRAFVPN
                     TDGLAFLSEGLLVGWPVWSISLAGALFGAAIGGGSLWLMGWLWERLRGVEAMGLGDVK
                     MMFMVGAYLGWPLTVVTIFLGVMSGAFIGVGLMLWRRERDMQMLLPFGIFLGLGAILS
                     LLAGQQIIDWYIGHFIP"
     gene            3382..4689
                     /locus_tag="VGB76_22115"
     CDS             3382..4689
                     /locus_tag="VGB76_22115"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY0101638.1"
                     /translation="MNSRRPIIALIVIAALIVAILTFVPYSLTMVRRAGQNVANAVQH
                     SLGKEQPAFEAEVRQKPIVPQFDVALWYGARGEEPEKHGVLIQSLDGERVFASHNADT
                     LFNPASLVKLTTTLIALKRLGADYRFVTRVYTDGSVDASGTLNGNLYLAGNDPTFGDT
                     SANLIAKELRARGINRVKEKILVAPGFSFNFNERAEDSAEYAARVMALKQSATGMAEP
                     EGTEIFALSSNPLREVLLYMNAHSNNFVADRLGAFLGGPSAIVRFLVEELKLPSEQVS
                     LETASGLYNNRMTPRGVLVVIKALTEEATRHGLKPEDLLPVAGCDWGTVRRRMEGTGF
                     ECSLVGKTGTLTTTDGGMSNLAGIVFTKNSGLLLFVILAQGNRIWEHKQMADQLLAET
                     IREQQPAPLFADTEKRRQLLPPNSLTIEQKSEVKGQKPVKAAH"
BASE COUNT          954 a         1427 c         1506 g          820 t
ORIGIN      
        1 gcctgccgcg caggacggac gatgcggagg gcttgctggc gcgcatcggc aacggccatc
       61 tcgtcgtcat gctcgaccgt ctggacgaat ggcagatcgc ttacgtcatc ctcaaaggca
      121 gctaccggca gatacgcgag gcagggatag acgcgctccg ccagtcgctc gtctcaattg
      181 cgccggaact cgcagaccgc gttgcgcact tgaaggagtg gaagcaagtc tcgatgctct
      241 ccgtcgagtc gagccgggtg gcgcagtggt atcagccggg tctgctgctc atcggcgacg
      301 ccgcgcacgt tatgtcgccc gtcggcggcg tcggcatcaa ctacgcgatt caggatgcgg
      361 tggtcgccgc gaacactctc gcagagcctc tgcgcgacgg acgactcgaa ctacgccacc
      421 tgcgcatagt gcagcgacag cgcgaatggc cgacgcgttt catccagaga attcaggcgt
      481 tcatacagca ggtgatgatc gccaacatcc tgagatcaaa tcaaccgctg caagtgccgc
      541 gcatcgtccg tctcttcctg cgcacgccgt tcctgagaga cattccggcg cgcatcatcg
      601 ccttcggcct ccggcgcgtc cacgtcaagc cgtcgctccg cccgcccgct tgaagagttg
      661 cgatagagga gcgcgaaata gcctagacgg ctccgctctc gacgcgagcg cgcgatcact
      721 tcatccagtt gggcgatgac ttcatcaata gtccgggcgg gcatagttta tcctgtcggg
      781 gcggaggctg gcgcacgacg aatgcgctag cctccggggt tttccagagg caggcggagc
      841 gtgaaaactg cgccgcccgc aggcgcgttg gcggcggaga gcgtgccacc gtgttccgtg
      901 acgacgcggt gcgcgagggc gaggccgatg ccgtgtcctt tgctcttggt ggtgaagaag
      961 ggaatgaaga tgcgttgcaa ctccgactgc gagatgcccg tgcccgtgtc ctctatggag
     1021 atgacgagtt gcttccggcc actgtcgcca gtctcgactt cgccatgcgc gctgacgcgg
     1081 cgttgcgccc gcgtttgtgt ctcttcaaag gcttcggcgg cgttgcgcag caggttgagg
     1141 agcgcctggc ggagcatcaa ttcgtcggcg cgcacagagg gaaactcgcc ggagaggtcg
     1201 aacgaaacgg ctcgctccgc gtagaaaggt tgcaactcgt cggcgcagtc tgtgagcagt
     1261 tcgcgcatgg agacttccga gagttggagc ggttgcggac gcgcgaagtt gagaaaggcc
     1321 gtgatcatct gcgtgaggtt gcgcacttct tgcagcagcg cggaggccgc gccgcgcccg
     1381 cgctcgtcga gttcgagatt ctgcaaaaac tgcgcgtagc cgtggagcgt ggcgagcgcg
     1441 tttttgaatt cgtgcgccag cccggcggac atctcgccga ggctttcgag gtttcttttg
     1501 agcgcgaggg cttcgcgcaa ctgcgccacc tccgtgatat ccgtgagcag acagagcgcg
     1561 ccgcgcgcac cgctccccgc ctgcgggtca atcggcgcga ccgtgacgcc gagacgacgc
     1621 acgcgtcctt cgcccgttgt cgctgcgacc tcttcacgac gataaagctc tccgctgagc
     1681 aaacaacgtt cgaccagatt ggtcagttca ggcgagagcc ggagcagcgt cttgagatgt
     1741 tgtccggtgc tgtttgattc aatttcgagc agggcgcggg cgggcggatt gatgacggtg
     1801 gcgaggccgc ccgcgtcgaa ggcgacgagg ccggatggga cgctcgccac cacgcgctcg
     1861 ttgaacatct ccgccgacgc ggcgcgcgcg ctcgcctgcg cgctcaattt ctccaactcg
     1921 cgctgctgcg cgcgcagttg cgccacgacg gcctgaaaag tctcaagcac aaactccgtc
     1981 tcatcacgcg cgccttcggg acgcgcagcc acgggcgcgc gttcggcctc gccgacgagt
     2041 tggcgatacg ggcgcaccaa ccagcgcggc aggagaaaga ttccgggcag gctgatgagc
     2101 agcgtgatcg taaagacggc gaagagcacg ggcgggcgca cgttgcggtc tggcggcgtc
     2161 atcgccacca gcgtcagcgt gaacaaattc gccaccagca acagcccgac gaccaacgcc
     2221 agcaccaact gcacgcgatt ttcgtaacga cccgacataa aagctgtgat gagtgatgag
     2281 tgatgagtga tgagtgaaag tcaatggaat taactcatca ctcattactc atcactcatc
     2341 acttcttaag gaatgaagtg tccgatgtac cagtcgatga tctgttgtcc ggcaaggagg
     2401 ctcaggatcg cgccgaggcc gaggaagatg ccgaagggga ggagcatttg catgtcgcgc
     2461 tcgcgccgcc agagcatgag gccgacgccg atgaacgcgc cgctcattac gccgagaaag
     2521 atcgtcacca ccgtcagcgg ccagccgaga tatgcgccga ccatgaacat cattttgaca
     2581 tcgccgagtc ccatcgcttc gacgccgcgc aggcgttccc acaaccaacc catcagccag
     2641 agcgagccgc cgccgatggc cgcgccgaag agtgcgcccg cgagcgatat tgaccacaca
     2701 ggccagccca cgagcagccc ttcgcttaaa aatgcgaggc cgtccgtgtt cggcacgaac
     2761 gcgcggacga tgagcgcgaa gaggaggccg gggagcgtga tcacgtcggg caggatcata
     2821 tgctcggcgt cgatgaagat gagcgcgacg agcgcggcga cgaagattag atcgaagggc
     2881 agggcgagcg tcagcccgtc gtgcaggaag acgagggcga agagcagcgc ggtcagggct
     2941 tcgatggccg ggtagcgcgc ggagatcgag acgccgcagg cgcggcagcg tccgcgcagg
     3001 acgaggtaac tcaggatcgg aacgttatcg taaggccgga tcggcgtgcc gcaggaaggg
     3061 cagcgagagt tgggaaaggc gatggattcc tcgcgcggca cgcgatgaat caccacgttc
     3121 aggaaactgc cgatgagcgc gccgaaggtg gaaacgagga tgagtgcgag gggaaagggc
     3181 atgagcgtcg aatcggcgaa agaagttaaa ttttgcagtt gagtcatcct gaaggtttag
     3241 tctatgagaa tcgagacgct gtctgacgcc cactccgcct gatcgcgccc ttgacgcacg
     3301 gcgcgaactt taagcatatt aagacttcgc acgggtttgc gaaagactta aattttaacg
     3361 aacgacggga aaggatcacg catgaattcg cgcagaccta tcatcgcgct catcgtcatc
     3421 gccgcgctca tcgttgccat cctcactttc gttccttatt cgctcacaat ggtcaggcgt
     3481 gcggggcaga acgtcgccaa cgccgtccag cactcgctcg gcaaagaaca gcccgcgttt
     3541 gaagcggagg tgcgccagaa gcccatcgtg ccgcagttcg acgtggcctt gtggtacggg
     3601 gcgcgcggcg aagaaccgga gaagcacggc gtcctgattc agagcctcga cggcgagcgc
     3661 gtcttcgctt cgcacaacgc agacaccctg ttcaatcccg cctcgctcgt caaactaacg
     3721 accacgctca tcgcgctcaa gcggctcggg gcggattatc gtttcgtcac gcgcgtctac
     3781 acggacggaa gcgtggacgc ttcgggcaca ctcaacggca atctctatct cgcgggcaac
     3841 gacccgacct tcggcgacac gtccgcgaat ctcatcgcga aagagttgcg cgcgcgcggc
     3901 atcaatcgcg tgaaggaaaa gatactcgtc gcgccgggtt tctctttcaa cttcaacgag
     3961 cgtgcagaag actccgccga gtatgcggcg cgcgtgatgg cgcttaaaca gtccgcgacc
     4021 gggatggccg aaccggaggg aactgagatt ttcgcgctct cctcgaatcc actccgtgaa
     4081 gtgctgctct acatgaacgc gcactcgaac aactttgtgg ccgaccgcct cggcgcgttc
     4141 ctcggcggcc cttcggccat cgtgcgcttt ctggttgaag agttgaagtt gccgtcggag
     4201 caagtctcgc tcgaaacggc ttcgggcttg tataacaacc gcatgacgcc gcgcggcgtg
     4261 ctcgtcgtca tcaaagcctt gaccgaagag gcgacgcggc acggactcaa acccgaagac
     4321 ctgctgcccg tcgccgggtg cgattggggc acggtgcgtc gtcgcatgga gggaacgggc
     4381 tttgaatgtt cgctcgtcgg gaagaccggg acgctgacga ccaccgacgg cggcatgtcg
     4441 aatctggcag gcatcgtctt caccaaaaat tccggcctgc tcctcttcgt catcctcgcg
     4501 cagggcaacc gcatctggga gcacaagcag atggccgacc aacttctcgc cgagacgatc
     4561 cgcgaacagc agcccgcgcc gctgttcgcg gacacggaga agcgtcggca gttgttgccg
     4621 ccaaacagcc tgacgattga acagaagtcg gaggtcaaag gtcagaagcc ggttaaagcc
     4681 gcgcactgaa aagcgcggtc ggcgtcg
//
LOCUS       DASWNS010000638        22357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_76198, whole genome shotgun sequence.
ACCESSION   DASWNS010000638 DASWNS010000000
VERSION     DASWNS010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 22357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22357
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76198"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..976
                     /locus_tag="VGB76_22120"
     CDS             200..976
                     /locus_tag="VGB76_22120"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY0101639.1"
                     /translation="MKVLITGGSGALGRILVRTAADAGHDVRGLSRGERPQSAPSAVE
                     WVRADLASGEGLSTALAGVEAIIHSASDPRRAKPVDVEGTRRLVEAARAAGVSHLVYI
                     SIVGIDDIPYSYYRRKREAEQIIEASGVPYTNLRATQFHSLVDFLISKAARLPLLLPL
                     PTDFKFHSVADAEVAARLVACLAKGARGRVEFGGPEVLTLGEMAATWLGVNGVRKRLV
                     RLPLPGAIAAGFRAGLNTTPDAERGTTRWRVWLEQNRDRR"
     gene            1253..1585
                     /locus_tag="VGB76_22125"
     CDS             1253..1585
                     /locus_tag="VGB76_22125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0101640.1"
                     /translation="MEPIQTNLLQGTLDLLILKALSLGGMHGLGISRRIEQITNGTFH
                     VKPGSLFPALHRMEEAGWLNSSWGESENNRRAKYYQLSQAGQRQLKEETREWDRIVLA
                     ITQALKAT"
     gene            1596..4241
                     /locus_tag="VGB76_22130"
     CDS             1596..4241
                     /locus_tag="VGB76_22130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0101641.1"
                     /translation="MPLWRWLSTLSRNIFHKARVDEELDEEVRAYLELLTDEKSAQGL
                     DAEQARRASVLEVGGIEQVKEQVRDVRIGATMESLLQDLRYGVRVLIKNPGFTAVAVL
                     TLALGVGANTAIFSVINAVLLRPLPYPEAQRLVWVWETHPNIKEEPASLPNFTDWKSQ
                     SQSFEGMAGLANASLALTGEGEPERIPTCFVVGDLFTVLGVSPALGRSFLAEEDKPGN
                     ERVVVLSYAIWQRRFGGDQNIVSKSITLSGNPYTVIGVLPQGFKHPQTGQRKEPELYA
                     PLGRAPESAGRRSDFLGVLARTKPGVPLKQAQAEMNTITARLAQQYPDSNANWGVTLL
                     PLHERVTGDVSRALWLLMGVVGFLLLIACVNVANLTLARSAARGQEIAVRRALGADRS
                     RLVRQFLTESVLLSFIGGTLGLLLAAWGIAALSALSPGNIPRLDEVTLNWPVLAFTLA
                     ASLMTGLIFGLFPALNATNPNLTESLKEGGRSSTEGSRSARVRRGLVVFEIAVALMLL
                     VGAGLMIRSFTHLQDVDPGFQPERVVAVDVGLPSAKYKEDERVVGFYDQFFGRVKAQT
                     GVESVAAISTLPLSGSGDIITFQVDGQAEPGGDASADAEYRIISPGYFSTMSIPLVKG
                     NDFTERDTKDAPYVMLVNETFARRFFPNEDAIGKRLNPSASSDAPWRTIVGIVKDVRL
                     QSLDTEPYPQMYAPLAQSPRRSMTMVVRTAGDPMNVLPFVRNELKTLDKDQPLYNVRT
                     MEQVLAESIARRRFNMMLIAIFAFLGLVLASVGIYGVISYSVTQRTHEIGIRMALGAE
                     ARDILKMIVKQGMGMVLLGIGLGLGGAFALTRVMSSLLFGVSPTDLTTFAGVPLILAM
                     VALLACYIPARRATRVDPMIALRFE"
     gene            4375..5205
                     /locus_tag="VGB76_22135"
     CDS             4375..5205
                     /locus_tag="VGB76_22135"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY0101642.1"
                     /translation="MRKVLARTVLTCIAFALAASAAFAQTNSSRSSLACRDQDNFGDD
                     ARARHCQVKEQTIQATGGTLSVNGMKNGGIAVKGWERNEILVRYRLQTYAPTQAEADS
                     LAAQIRVTTAGGQVRAEGPENDGAARWDVGYEIFVPRQANLSLQTHNGGISLSDVRGQ
                     INFEARNGGVALKNMGGNVKGETVNGGLHVELTGNNWEGEGLDVRTTNGGLSITVPEN
                     YSAHLETGTVNGGLVVSPAIAEVTRKTKQLSLDLGAGGTNLRIHTTNGGVSIKRRESR
                     "
     gene            complement(5404..5658)
                     /locus_tag="VGB76_22140"
     CDS             complement(5404..5658)
                     /locus_tag="VGB76_22140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101643.1"
                     /translation="MVLGLDVGEGDSGDPVASMKSLKLTYGLLLKGNAIGKSYMANVL
                     PTLYVVGADGRILHAGFGCCKDLGAGLTELIGKHLNSQAR"
     gene            5687..5944
                     /locus_tag="VGB76_22145"
     CDS             5687..5944
                     /locus_tag="VGB76_22145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101644.1"
                     /translation="MGEILSSDTEWQDYRARKLVLRIAGRADEPESFAAARRCLADAM
                     ELVAQRLNDMWTDERYVRASLDDEFLSEGDIESEEEPSEKE"
     gene            6248..6403
                     /locus_tag="VGB76_22150"
     CDS             6248..6403
                     /locus_tag="VGB76_22150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101645.1"
                     /translation="MLLPRRFQLFDRLVRQRLILYRMHGQIDALPAADRANKVTDALA
                     QQLFAGD"
     gene            6629..7018
                     /locus_tag="VGB76_22155"
     CDS             6629..7018
                     /locus_tag="VGB76_22155"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY0101646.1"
                     /translation="MSKKEASTLSRRERQIMDIIYSRGQASATEVMESLPDPPSYSAV
                     RALLRVLEEKGHLRHKQDGQRYVYSPTVARERAKRSALRRVLQTFFDDSAEDAVAALL
                     DISQEHLSDDELKRMENLIKQARREGR"
     gene            7022..9166
                     /locus_tag="VGB76_22160"
     CDS             7022..9166
                     /locus_tag="VGB76_22160"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY0101647.1"
                     /translation="MSYCWNILTSHDSYELLSLLALTATKATLLLACAALLCFAFRRF
                     SAATRHLLWASALCASLLLPFLSFIKMWELPILPAQMLVSDSAAAKELPGRDEALAMP
                     EAQGAEGFSPSLDSGVGMQKVTGAQTNAESLEARVPAPDASEFPLTTPQKTTSLLPQL
                     LNIAVAVWVVGVLLFLLKLLAGFIATNLLSRRAVEFKDPSLTALFSSLLLEVNLKSTV
                     RLLRSERTSMPIVYGILRPAVLLPAAAEAWSEERQRMVLLHELTHITRRDCLTQMIAQ
                     MACAFYWFNPFIWFAARRLRVEREQACDDYVLSIGTKPSDYANHLLDIARSMQERSIF
                     EWSQTTSVAMARRSQLEGRLLAILSKENKRGAVSRLAAMSLIALICFLLVSLAAVRPT
                     VIHAQNPRTANDASTDERADTASALPDSLVAIVAGHGGDAAATGAETREAGRDQVAAA
                     VATVNSAPEQDSNGVQIEDGISGNINGEVRRIVEPEAAETAAQQATTLVHMLEVKPLV
                     RQEANSFINAGHRPEPTPRTQDKRGDFIDEMASAGYTNLSIDELIRLKTAGVTADYVR
                     SLRALGLANLASKELASLAIHGVTAAYIQSIRAAGYNDLSAKELTTFRIHGITPEYIK
                     TLRDAGYGNLTAKQLVEFAIHGVTPAFIGSIRAAGYGNSSPKELVSLRIHGVTPEFIR
                     KARSRLGEPTLKQILSLKMIGVLDDEKDKEKK"
     gene            9192..10235
                     /locus_tag="VGB76_22165"
     CDS             9192..10235
                     /locus_tag="VGB76_22165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101648.1"
                     /translation="MKQAQTFTCKSSTLKRHARRRAGSLLVAFLVTSTLAATTAGNAK
                     LTSANTAMQAAITGEWLVDFNRKNADEVQFTVIRRSERGGQHNSSNGVPLSELQGLTR
                     QQATGARTEVNFRIVREAGTFQCEGFFQEGRGAGHWTLTPNQSFVSAMRGRGYDNLSA
                     DDLFSAALFDINVKTIEDLKSAGYDRLSFQELVEASIFKVTGDFAREMKSAGFENLTF
                     KQLVEARIFKVDSQYAKEVQSMGFGAQPLKTLVEMRIFKITPEFISEMRSMNFDDLSL
                     RQLIDMRIHKITPEFVNGVKGEGFASISPRQAVELKIHGVDADFIRRVKAKGFNNVSL
                     NKLVELRIHRVIE"
     gene            10276..11319
                     /locus_tag="VGB76_22170"
     CDS             10276..11319
                     /locus_tag="VGB76_22170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101649.1"
                     /translation="MKKFQTAESHLLTTKQLRISSLLLSGLFAFSALVYANVSSASAK
                     GGLDPVQAQDTLSGQWTAEVRRDKPGEIQMTYHRHSETGGFNISGDTFALSELQGLSA
                     ETALSARTNVNFKLVRQAGTFACEGYFREGRGAGFWTFTPSQSFVSAMRERGYSNLTD
                     GDLLRAALHNLTTNYIEDLKSAGYDRLEFKQLLRASSHSITPAFIREMQSAGYQGLTM
                     EELVRARNHNIDSRYVGEVRAMGFDKQPLAKLIRLRNHQITQDFINRMRSAGFDGLSI
                     EQLIRLKNHEITPEFVNGVKAEGYADIPVETAVRLKNHEVDRDYIRRVKARGFTDLTL
                     EQIIKLRSREIIK"
     gene            11510..14095
                     /locus_tag="VGB76_22175"
     CDS             11510..14095
                     /locus_tag="VGB76_22175"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HEY0101650.1"
                     /translation="MKNISGLCLVILLCALNSSAQTLPPNPAEAGASVANPLASPPAS
                     PSLSASIEAATATATPGVETPVSPYARKDATVRIPRFESAPLIDGQLNDAVWRSATVF
                     GDFLQTQPGDNVAPTHPTEALMGYDAKNLYLAFRVKQDRDKVRATVARRDNIFNDDYV
                     LVYIDTFNDQRQAYVIFFNPLGIQADGTYTEGRGEDYSVDLLMESKGVLTEDGFTIEA
                     AIPFKSLRYEAGKNKQWGLHIFRRVKYNNNEYNSWMPNNRSISGSLNQAGRITGLEDI
                     STTRQLEFNPTLTLSESGRRTRFTFDQNPAGRFVNEGLKPELGMTAKFSLSPTVTLDF
                     AYNPDFAQVEADAPVTTANVRFPIFFAEKRPFFLERIDIFQSGLNIVNTRAIADPDVA
                     LKLTGRRGRNTFGLLFASDNAPGNYSKDERQGLDLCRAANPGNQCGIEPFVDKNSNIG
                     VVRLKRDIGRQHNLGFFATTYSFPERHNHTAGFDGRFRLDEKTVAEFHVTGTNSRGHF
                     YDPDMDGHLYRTGNGFGYRAYLERSDRNWYMNYLAIGRTRDYRADVGFTQRVDTNYIG
                     SFIQYQTDRDAKKSIIYRRLNNATNATYDWKGRTQGLQSNTQGMLALQRQIFVGLGLE
                     YGYERVFEHEFGARRTATRAGRFFGESSERSSHRGEIYGFIEATPVKQLFIYTVLSHS
                     DGNLDYDLGAGPKYPRVSPPYLAFVEENERRKREGRVDLLPRPGLDPGAGNQLFIESS
                     IRYQPTVALQAQLNYTRTRLTRHDTGRVAFDDNVFSLRSTYQFTRDTFARLRLDYSNI
                     STRFRPQLILGWTPSPGTALYAGYNDDLNYHGYNPYTGVHEPGFRGNGRTFFIKASYL
                     FRKSF"
     gene            complement(14327..16393)
                     /locus_tag="VGB76_22180"
     CDS             complement(14327..16393)
                     /locus_tag="VGB76_22180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101651.1"
                     /translation="MKDRNSELSSKPSDIEIPHAMGMVSWYRPTVLVRTAFDTLVSTI
                     FGRHSDYRLLEALATDSEKKSEVFYDYTHHYKVRHEGDIRDNENANWYVRDEDGEYEK
                     DERRKREEIWIDYIADTGDGWNSTYAVAYTATKDELKFKVDNSEKELVTRRGHLLVFG
                     GDQVYPVASRKAYANRLIKPYQAALGSTAPGDTSGQTDPHPFLFATPGNHDWYDSLVS
                     FTRLFCARRWFAGWLTRQTRSYYALRLPHNWWLIGIDVQLGSDLDGAQVRFFEKVVKQ
                     MEEGDRVILCTAEPHWVLGDLYQKYDEQYNDSNLKFFEEKILGDKAEVHVYLSGDLHH
                     YRRHEHVTKHGEKIQKITAGGGGAFLHPTHRMGRINPEHDPERETVTDSVMSREERAE
                     RKFKTGTEGHTPKVRVFRLEKSFPEWDESKKACRRNLFFLIYNPFFGLLTAILYWLSS
                     WVVVPYTQEFFKAQAADSTRNFWETLSHVVASQFPDAGLTFWLALIITGFLLFTDTHS
                     KWYRWLAGLTHGITHVVALILLCVLAYWTASRLGLGAQVSPGIIKLLLLHLMMAAIIF
                     VGGWIVGSFIMGIYLWVSMNIFGRHYNEAFSSLKIEDWKHFLRLHIGQDGALVIYPVG
                     IPKVPRAWKRNTDFKAHRKATSVPAETEGGDDKLQVLLIEQPLRVAGKSGDAATITAQ
                     TRRTED"
     gene            complement(16783..17832)
                     /locus_tag="VGB76_22185"
     CDS             complement(16783..17832)
                     /locus_tag="VGB76_22185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101652.1"
                     /translation="MTETKGKQKTEIKTRQPQKKTQDTFANLRKPHPVEELLGLTQPT
                     PATPLTPLTPPTPQAIAPARDFARVANSIVREAVPSGAFTGKSKQLYDYLYAKTRGAV
                     VPKRSVRMTKTAIMKGSHIGSERTMYKNLRRLEAAGLLQIRSIAGEQDGSEYTIILPE
                     EVAPLTPPTHPTHSPHSLQKVVTPLTAESGVSGVSLSAADSTVSGELKTSFKTKEESI
                     DDEAARRLVELFLQAEKELTGKNSANAAQWAELAELLVTELKIAAGRTTVSNVPAFLT
                     EHLRRRLWKLDKKRASEIAVEAEEQGIQPTLSEEEKRRCPDCAGTNFWYPEGTDKGVA
                     KCRHTKLINTQTEEK"
     gene            complement(17829..18620)
                     /locus_tag="VGB76_22190"
     CDS             complement(17829..18620)
                     /locus_tag="VGB76_22190"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY0101653.1"
                     /translation="MKTIAIANQKGGVGKTTTATNLAAGLAIRGYKTLLIDLDSQCNA
                     TFTFLDQNLIKTTLADVLVGYQERPSLIDAIYETHIEGLDIAPSHIRLALLERSVQIE
                     EHYRLKDSLESLEAYDFAVIDCPPSLGMTLTQALLASSHAIVPVAAQFYPLEGVVDLT
                     GTMQATRRLNPSLSILGYLMTYFDGRNRICGEALAKMQEMFDGQVFETVIRTNVKLQT
                     APSLRKSIFEHAPDSHGSKDYDSLTDEVLQRLKMDSSLRLVQEAG"
     gene            complement(18617..18832)
                     /locus_tag="VGB76_22195"
     CDS             complement(18617..18832)
                     /locus_tag="VGB76_22195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101654.1"
                     /translation="MIKTNPTPQTISISEAQTRLGVSRTALWRLIRSYNIETFQDVLD
                     ARVKRVRVSDIQRVLDDADRVRRGIAA"
     gene            complement(19045..21570)
                     /locus_tag="VGB76_22200"
     CDS             complement(19045..21570)
                     /locus_tag="VGB76_22200"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0101655.1"
                     /translation="MRSTRRFVATLFLALAFMCASAAAQGVAPLTLEPVLSNLSSPVL
                     VTNARDGSNRLFVVEQTGRVQVLQPGASTPSVFLDLTAKVLSGGERGLLGLAFHPQFR
                     ANRRLFVNYTRQPDGATVIAEYRASATNPNAVEAAETVLLVIPQPFANHNGGMIAFGA
                     DNLLYIGMGDGGSANDPQNRAQNVEELLGKMLRINVDQASGSTLYSSPPDNPFFGATP
                     GRDEIYAVGLRNPFRFSFDRATGQLYAGDVGQGVREEIDIITRGGNYGWRVFEGTRCT
                     GLGPAQCNAANFQSPVAEYDHTGGRCSVTGGYVYRGTRATIPTGAYIYADFCTGEMFL
                     LQGTAQSFLQDTNINISSFGEDETGELYVVGLGGTVHRLVNSCSYTISPATRTSPANG
                     ETLAVNVTTQGGCGWAAVSNSGFITVSSGASGTGSGTVTLSVSSNGSGVPRTGTVFIA
                     GQTLTITQPEVIPEVPTLQFSQPAYAFSEGAGRAVAVITRAGDSSFSVAVKFETVDDP
                     AAVSCATANGTAYARCDYATTVDTVTFAPGEESKQISIPLVDDAHVEGNETVRLRLSD
                     ATGLAALGAQSTATLTISDNDTTTGANPIFNTTFFVRMHYLDFLSREPEPGEPWSAVL
                     NGCPNVNNNPACDRITVSQGFFGSREFRLKGFFVYNFYRVALNRRPEYAEIIPDMRSV
                     TGQTSADTYARRAAFPVAFVQRPAFKAAYDSLSNTAYVNALLDRYGLQQITTPDPQNP
                     EGETLLTLTRADLINRLGTTGAQALTRAQVLRAIVESEEIAAHEYKGAFVAMQYYGYL
                     RRTPEERGYQDWLRVITEDPNNIRIMVNGFVNSTEYRLRFGNP"
     gene            21864..22163
                     /locus_tag="VGB76_22205"
     CDS             21864..22163
                     /locus_tag="VGB76_22205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101656.1"
                     /translation="MRKLLALTLSLASIGFVASPAEAKASAANIAPAAANAAAASPAS
                     VQWGRNNRRINRRARTVTQSRLVRIGRRTFRETYQITYWPNGRTETRLISRVRVR"
BASE COUNT         4719 a         6316 c         6560 g         4762 t
ORIGIN      
        1 ccagaactgt gggcgacaaa gtgacggcgg aagtgattcg taaatacatc caacgtcaca
       61 aagaaaccga agatacgcaa atagacctgt tcgactaaat cactctaaag ccccaccgct
      121 tgcggtgggg tacttttact catcacaggc gcagtcagcg atatgctcct gtcccgcgtc
      181 ggtttaggag tcgctcccga tgaaagtact gatcacaggc ggcagcggcg cactgggtcg
      241 catacttgta cgcaccgcag cggacgcggg gcacgacgtt cgcggtctga gccgtggcga
      301 gcgtccgcag tccgcgccgt ccgctgtcga gtgggtgcgc gccgacctcg catcgggtga
      361 aggtttgagc acggcgttgg ccggggtgga ggcgatcatt cactctgcct cagacccgcg
      421 ccgggcaaag ccggttgacg ttgagggaac gcgccgcttg gtcgaggctg cgcgcgcggc
      481 gggcgtctcg cacctcgtct acatctccat cgttggcatt gacgacattc cctacagcta
      541 ctaccggcgc aagcgcgagg cggaacagat catcgaagca tccggcgtgc cctacacgaa
      601 cctgcgcgcc acacaattcc actcgctcgt agattttctt atctcgaagg ccgcccgtct
      661 gccgctcctg ttgccgctgc caaccgactt caaattccat agcgtagctg acgcggaagt
      721 ggccgcgcgc ctcgtcgcgt gtctcgcgaa aggtgcgcgt ggacgggttg agttcggcgg
      781 gccggaagtg ctcacactcg gcgaaatggc tgcgacgtgg ttgggagtaa acggtgtacg
      841 caaacgactg gtgcgcttgc cgctgccggg ggcaatcgcg gcgggcttcc gcgctggcct
      901 gaacacgacg ccggacgctg agcggggcac gacacgctgg cgcgtgtggc ttgaacaaaa
      961 cagagatcgg cgatgacgag tccgacctgt gggatgaacc tgtacagggt cgggctgaat
     1021 aggtttagtg taaacaacac gccgcgtcag gcagagtaac gcaccctccg gacggaaact
     1081 acttcgatag gtgatagcca atcgtctttg gcagccaatc ctggctgcga gtagtttggc
     1141 gtgccgcact atttagaaaa tggacacaca gcccgtcgat gccgccacct ttgtattgac
     1201 attctatacg tgtgcgcgta ctctcgtaca gagcatctat aggaagcatc gaatggaacc
     1261 tattcaaaca aacctacttc agggcacgct tgatttattg atcctcaaag cgctctcgct
     1321 gggcgggatg cacgggcttg gtatttcgag gcgcattgaa cagatcacaa acggaacttt
     1381 tcacgtcaag ccaggctctc tattcccggc actccatcga atggaggagg cgggctggct
     1441 caattcctcc tggggcgaat ctgaaaataa tcgccgcgca aagtattacc aactgagcca
     1501 ggctggacag aggcaactta aagaggagac acgggaatgg gatcgcattg tgctcgccat
     1561 cactcaggct ttaaaggcga cttagaggtt taaagatgcc gctctggcgt tggctctcca
     1621 cactgtcgag aaatattttc cacaaggctc gcgtagatga agagttggac gaagaggtgc
     1681 gtgcgtatct ggagttgctg acagatgaga agagcgcgca ggggttggat gcggagcagg
     1741 cacggcgcgc ctccgtgctt gaagtgggcg gcattgaaca ggttaaagaa caggtgagag
     1801 atgtgaggat cggagcaaca atggaaagtt tactgcaaga cttgcgctac ggcgtgcgcg
     1861 tgctaattaa aaaccccggc ttcacggcag tcgccgtgct gaccctcgct ttgggcgtcg
     1921 gggcgaacac agcgatcttc tcggtgatca acgccgtgct gttgcgtccg ctgccttacc
     1981 cggaggcgca aaggctcgtc tgggtttggg agacgcaccc gaatatcaaa gaggaaccgg
     2041 cttcgctgcc gaatttcact gactggaaaa gtcagtctca gagctttgaa ggcatggcgg
     2101 gcttggctaa tgcgagcctc gcactcacgg gtgagggcga gccggaacgc atcccgactt
     2161 gcttcgtcgt cggcgatctc ttcacagtac tcggcgtcag ccctgcgctc ggtcgcagct
     2221 ttctggcgga agaggacaag cccggcaatg aacgcgtcgt cgttctcagc tacgcgatct
     2281 ggcagcgacg tttcggcggc gaccagaaca tcgtgagcaa gtccataacc ttgagcggca
     2341 acccgtacac ggtaatcggc gtcctgccgc aaggcttcaa acatccgcag acgggtcaac
     2401 gcaaggagcc ggaactttat gcgcctttag gccgcgcgcc ggagtctgcc gggcgtcgct
     2461 ccgactttct gggcgtcctg gcgcgcacga aaccgggcgt cccgctcaag caggcgcagg
     2521 ccgagatgaa taccatcact gcgcgcctcg cacagcagta tccggactcg aacgcgaatt
     2581 ggggcgtgac tttgctgccg ctgcacgaga gagtcacagg cgacgtaagc cgcgcgctgt
     2641 ggctgctgat gggcgtagtc ggcttcctgc tcttgatcgc atgtgtgaac gtggcgaacc
     2701 tgacgctggc gcggtcggcg gcgcgcggtc aggagatagc agtccggcgt gcgctcggcg
     2761 cagaccgttc gagactcgtc cgtcaatttc tgaccgagag cgtcctcctc tcttttattg
     2821 gcgggacgct tggtttactg ctggcggcgt ggggcatcgc cgcccttagc gcgcttagtc
     2881 ccggcaacat cccgcgtctg gacgaggtga cattgaactg gcctgtgctc gccttcacgc
     2941 tcgccgcttc actcatgacg ggtcttatct tcggactctt cccggccttg aacgcgacga
     3001 atcccaacct caccgaatcg ctcaaggagg gcggtcgcag ttcgacggaa ggcagtcgca
     3061 gcgcacgcgt gcgccgtggg cttgtcgttt tcgaaatcgc cgttgcgctc atgctgctgg
     3121 tgggggcggg gctgatgata cgcagcttca cgcacctgca agatgtcgat ccgggctttc
     3181 aacctgaacg cgtggtggcc gtcgatgtgg gcttgccgtc ggcgaagtac aaggaagatg
     3241 agagagtggt cggtttctac gatcaattct tcggtcgcgt gaaagctcaa acgggcgtcg
     3301 aatcggtcgc cgcgatcagt acgctgcctt tgagcgggag cggcgacatc atcacttttc
     3361 aggttgacgg gcaagccgag ccgggtggcg atgccagcgc agacgcggag tatcgcatca
     3421 tcagtcccgg ctacttcagt acgatgagca ttccgctcgt caagggcaac gacttcaccg
     3481 agcgcgacac gaaagacgct ccctacgtga tgctcgtgaa cgagactttt gcgcgccgct
     3541 tcttcccgaa cgaagatgcc atcggcaagc gcctcaatcc cagcgctagc tccgatgctc
     3601 cctggcgaac catcgtcggc atcgtcaaag atgtcaggct tcaaagcctc gacacagaac
     3661 cttacccgca gatgtacgcg ccgctcgccc aatcgccgcg ccgctcgatg acgatggttg
     3721 tgcgcaccgc cggcgacccg atgaacgtgc tcccgttcgt caggaacgaa ctcaagacgc
     3781 ttgataaaga tcagccgctc tacaacgtac gcacgatgga gcaggtgttg gccgagtcta
     3841 tcgcgcgccg acgcttcaac atgatgctca tcgccatctt cgccttcctc ggcctcgtcc
     3901 tcgcctcggt cggcatctac ggcgtgatct cctattccgt cacgcagcgc acgcacgaga
     3961 tcggcatccg catggctctg ggcgcggagg cgcgcgacat tctcaagatg atcgtcaagc
     4021 aaggcatggg catggttctg ttgggcatcg gtctgggact cggcggggct ttcgccctga
     4081 cgcgcgtgat gtcgagtctg ttgttcggcg tcagcccgac cgaccttact accttcgcgg
     4141 gcgtccctct gattctcgcg atggtggctc tgctcgcctg ttacatcccg gcgcggcggg
     4201 ctacgcgcgt cgatccgatg atcgctctga gattcgagta agggagactt tttgcgacgc
     4261 tgccatattt tctgccacaa agtggacgga cgcggtgttt actagattga acgacgaagt
     4321 ttttcaggct ttgctcggac aagctaatcc gccttttgag gagaaaatat gatattgaga
     4381 aaagtattgg cgcgcacggt tctgacctgc atcgccttcg cccttgccgc gtcggcggct
     4441 ttcgcacaaa caaacagttc gcgctcctcg ctcgcctgcc gcgatcagga taatttcggc
     4501 gacgatgcgc gcgcgcgtca ctgtcaggta aaggaacaga ccattcaggc gacgggcggc
     4561 acgctcagcg tcaacgggat gaagaacggc ggaatcgccg tcaagggttg ggagcgcaac
     4621 gagattctgg tgcgctatcg cctccagacg tatgcgccga cgcaggccga ggccgacagc
     4681 ctcgctgctc aaatcagggt tacgacggca ggcgggcagg ttcgcgccga aggccccgaa
     4741 aacgacggcg ctgcccgctg ggatgtgggc tatgagatat tcgtcccgcg tcaagcgaac
     4801 ctttcactcc agacacacaa cggcggcatc agcctctcgg acgttcgcgg gcagattaac
     4861 ttcgaggcca gaaacggcgg cgtcgccttg aagaatatgg gcggcaacgt caaaggcgag
     4921 acggtcaacg gcggcctgca tgtagaactg accggcaaca actgggaggg cgaaggactc
     4981 gacgtgcgga cgaccaacgg cggcctgtca atcacggtgc cggaaaatta ttcggcgcac
     5041 cttgagacgg ggacggtcaa cggcggcctc gtcgtgagtc ctgccatcgc cgaagtgacg
     5101 cgcaagacga agcagttatc gctcgacctc ggtgcgggcg gcacgaacct ccgtattcac
     5161 acgacgaacg gcggcgtctc gatcaagcgt cgcgagtcga gataaaccat caacacgtta
     5221 aagcctgtgc tggccgaccg tcgcttccga tactttctca tgttcgttcc ctaagctttg
     5281 ctaaagcctg ccgggagtgg agacagcaac actcccggca ggggaactgc atcagcacag
     5341 acgattcacg gtcgcgcatc aagcgactgc ttccgtagag cgaacgtttg ttgagcgaaa
     5401 aggctaacgg gcttgactgt tcaaatgttt cccgatcagt tcggtcagcc ccgccccaag
     5461 gtctttgcag cagccgaacc cggcatgcag gattctaccg tccgcgccga cgacgtagag
     5521 cgtcggcaac acgtttgcca tataactctt gccaatcgca ttccctttaa gaagtaggcc
     5581 gtaagtcaat ttcaagctct tcatggaggc gacggggtcg ccgctgtcgc cctcacctac
     5641 gtcgagtccc aacaccacga cgcctctgtc cttaaacttt tcatgtatgg gggagattct
     5701 ttcttcggat accgagtggc aggactacag ggctcggaaa ctcgtcttga gaatagccgg
     5761 tcgtgcggat gagccggaaa gcttcgccgc agctaggaga tgcttggccg acgcgatgga
     5821 actggtggcg caacgcctta acgacatgtg gaccgacgag cggtacgtgc gcgcgtctct
     5881 cgacgacgag ttcttatcgg aaggagatat tgagtccgaa gaagagccta gcgagaagga
     5941 gtaattgcct ccaaatagtc ttgcgggctg atgtgagatc gcataagtct ctggtagaga
     6001 ttcgacggct catggaggta atctttagag gagccgtcct ctccgggact taaaaggggt
     6061 aaatctgaga gggcttcgct gacggtgatg gcaggggcaa gtacggaaga gaacggctct
     6121 ttagaaaccc cgtacctcgt aacgggtacg ggcgactcta tgcctttact acgtccagag
     6181 tcgccgacta atacgatacg catcctcctt tggcactctt cggcacgctt ttagcccggc
     6241 cagctttgtg cttctgccac gccgcttcca gttgtttgac cgactcgtcc gtcaacggct
     6301 catcctctac cggatgcacg gccagatcga cgccctgccc gccgcagacc gtgccaacaa
     6361 agtcactgat gcactggcgc agcaactgtt cgcgggtgat tgacggagtg aagtgaaaag
     6421 gtgtcctgcc cgcccctttc tacggctgta gccgtactac gacgcacgta gaaatgagtc
     6481 aagaagaatt tatccagaca cgaatttttg gatttgaata atcgcgattg acttcccgga
     6541 aggttggtgc taaaaaacca gcacctgctg gaaatttagc atccggtgtg aaatcgaggg
     6601 acaaattatc ttaagaaagc aagaattgat gagcaagaag gaagcaagca cgctgtcacg
     6661 gcgggagcgg cagatcatgg acatcattta tagccgcggc caggccagcg cgactgaagt
     6721 tatggaaagt ttgcctgacc ccccaagcta ctcggcggtg cgagcgctgc tgcgcgtgct
     6781 cgaagaaaag ggtcatctgc ggcataagca agacggacag cgctatgtct attctccgac
     6841 ggtggcgcgc gagcgggcga aacgttcggc gctgagacgc gtgctgcaaa cctttttcga
     6901 cgattcggcg gaagacgccg ttgcggcttt gctcgacatc tcgcaggaac atctgtcaga
     6961 cgacgaatta aaacgcatgg agaaccttat caaacaggct agaagggaag ggcgttaaat
     7021 gatgagttac tgctggaata ttttaacgtc gcacgacagt tacgaactcc tgtcgcttct
     7081 cgcgttgacg gcgacgaaag cgactctgct cctcgcttgc gccgccttgc tctgttttgc
     7141 ttttcgtcgc ttttcggcgg cgacacgtca tctcctgtgg gcttccgcgc tgtgcgcctc
     7201 gctgctgctg ccttttcttt ccttcataaa gatgtgggaa ttgccgatct tgcccgcaca
     7261 gatgttggtg tcggattctg ccgccgcgaa agagctgccc ggaagagatg aagcgcttgc
     7321 aatgccagag gcgcaagggg cggagggctt ttcaccttca ctcgattcag gcgtcgggat
     7381 gcaaaaagta accggagcgc aaactaatgc agagtcgctt gaagcgcgcg tgccagcacc
     7441 agacgcttct gagttcccgt taacgacgcc ccaaaagacg acttcgcttt tgcctcaatt
     7501 gcttaacatc gctgtcgccg tgtgggtcgt cggagtgttg ctgtttttac ttaaattatt
     7561 ggccggattc atcgccacaa atttgttgag ccgccgcgcc gtcgagttca aagacccgtc
     7621 gcttaccgcg ttgttttcgt ctcttctgct tgaggtcaac ttgaagagta cggttcgctt
     7681 gttgcgcagc gaacgcacgt caatgccgat tgtgtacggg attttgcgac ccgccgttct
     7741 gctgcctgcg gcggctgaag catggtctga ggaacggcaa cgaatggtgt tgctacacga
     7801 actgacgcat atcacgaggc gggattgttt aacccagatg atagcgcaga tggcatgcgc
     7861 gttttactgg ttcaacccgt tcatctggtt cgccgcgcgt cgcctccgcg tggaacgcga
     7921 acaagcctgc gacgattacg tgttgagcat cggcacgaag ccatccgact acgctaatca
     7981 tctgttagac attgcgcgtt cgatgcagga acgctcgatc ttcgagtggt cacaaacaac
     8041 cagcgtcgcc atggcgcgac ggtcacagtt ggaagggcgt ctgctggcga ttcttagtaa
     8101 agagaacaaa cgcggcgcgg tgtcgcgcct cgcggctatg agcctcatcg cgttgatatg
     8161 ctttctactc gtctcactcg cagccgtgcg cccgacggtt attcacgcgc agaatccgcg
     8221 aactgcaaat gacgcttcaa cggatgaaag agcggacacg gcgagcgcct tgccggattc
     8281 gcttgtcgcc atcgtcgcgg ggcacggagg cgacgcggcg gcgacgggtg cagagacgcg
     8341 tgaggcggga agagatcaag tggctgctgc cgtcgctacg gttaatagtg cgccggagca
     8401 ggactcgaac ggcgtgcaaa ttgaggacgg aatcagcggg aacataaacg gagaggtcag
     8461 acgtattgta gaaccagaag ccgccgaaac tgccgcacaa caggccacga cactggttca
     8521 catgctggaa gtcaaaccct tagtcaggca ggaagcgaac tcttttatca atgcgggaca
     8581 ccgcccggag cctacacccc ggactcaaga caagcgcgga gattttatcg acgagatggc
     8641 ttcagccggt tacacgaatc tttcgattga tgagttgata aggctcaaga cggcgggcgt
     8701 cacagccgat tatgtgcgga gtttgcgcgc gctcggcctt gccaatctgg cgtcgaaaga
     8761 actggcctcg ctggcaattc atggcgtcac agcggcttat attcagtcga tccgcgcggc
     8821 aggttacaac gatctatccg cgaaagagtt aaccactttt cgcattcatg gcatcacgcc
     8881 cgaatacatc aagactctac gtgacgcggg ctacggcaac ctgactgcga agcaactggt
     8941 cgagtttgcg attcatggcg ttacgcccgc cttcatcggt tcaattcgcg cggcgggcta
     9001 cggcaattcg tcgcctaaag aactcgtctc tttacgcatt catggcgtga cgcccgagtt
     9061 tattcgcaaa gcgcgcagcc gtctgggaga accgacactc aaacagattc tctctctcaa
     9121 aatgatcggc gtcctcgatg acgagaagga caaagagaag aaataaggat cggaagcgaa
     9181 ggagtttaat tatgaagcaa gcgcaaacat tcacgtgtaa gtcttctacc ctcaaacgac
     9241 acgcccgacg acgcgccggt tcgctgctcg tcgcctttct cgtcacttca actctcgccg
     9301 ccacgacggc aggcaacgcc aaacttacca gcgcgaacac tgcaatgcaa gctgcgatta
     9361 cgggcgaatg gctcgtcgat tttaaccgca agaacgcaga cgaagtgcag ttcacggtta
     9421 tccgtcgttc ggagcgcggc ggccagcaca actccagtaa cggcgtcccc ctcagcgaac
     9481 tacaaggtct gacgcgccag caagccacag gcgcgcggac ggaggttaat ttccgcatcg
     9541 tgcgcgaagc gggcaccttc cagtgtgagg gattttttca ggagggcaga ggcgcgggac
     9601 attggactct aacgccgaac cagagtttcg tttccgcgat gcggggtcgc ggttacgata
     9661 atctctccgc agatgacctg ttttccgccg cgctcttcga catcaacgtc aagaccatcg
     9721 aagacttgaa aagcgcgggc tacgaccgcc tctcgtttca ggaactcgtg gaagccagca
     9781 tctttaaagt gacgggcgac ttcgcgcgcg agatgaagtc cgccgggttt gaaaatctaa
     9841 ctttcaaaca gcttgtcgaa gcgcgcatct tcaaagtcga tagtcagtat gcgaaggaag
     9901 tgcagtcgat gggcttcgga gcgcagccgc taaagacgct ggtggaaatg cgtatcttta
     9961 agatcacgcc tgagttcatc agtgaaatgc gctcgatgaa cttcgatgat ctctcgctca
    10021 ggcaattgat agacatgcgc attcacaaaa taactcctga attcgtcaac ggtgtgaaag
    10081 gcgaaggctt tgcctcgatc tcgccgcgtc aggcggtcga actgaaaatt cacggggttg
    10141 acgcggattt tatcaggcgg gtgaaggcga aggggtttaa taacgtctcg ctgaataaac
    10201 tcgtcgagct gcgcattcac agggttatcg aataaccgtc gaatcaacat agacagacag
    10261 agcgggaggt gagttatgaa aaagtttcag acggcagaga gtcacttgtt aactacgaag
    10321 cagttgcgta tttcgtcgct gctcctgagc gggctgtttg ccttttccgc tttggtctat
    10381 gccaacgtca gcagtgcgag cgcaaagggc ggcctcgatc cggttcaagc gcaggatacg
    10441 ttgtccgggc aatggactgc cgaggtgcga cgcgacaagc cgggcgaaat acagatgacg
    10501 tatcacaggc attcggagac gggcggcttc aatatatcgg gcgacacatt cgcgctcagc
    10561 gaactgcaag gtctgtctgc cgagacggcc ttgtctgcaa ggacgaacgt taatttcaaa
    10621 ctcgtgcgac aggccgggac gttcgcctgc gaaggctatt ttcgcgaagg caggggcgcg
    10681 ggtttctgga ccttcacgcc gagccaaagt ttcgtctcgg cgatgcggga gcgcggttac
    10741 agtaacttaa ccgacgggga tttgctgaga gccgctctgc ataacctgac gacaaactac
    10801 atcgaagatt tgaagtcggc aggctacgac cgcctggagt tcaaacaact cctgcgggcg
    10861 tcgagccact ccatcacgcc cgccttcata cgcgagatgc aatccgcagg ttatcagggt
    10921 ctaacgatgg aagaactcgt ccgcgcgcgc aatcacaaca tcgacagccg gtacgtcggc
    10981 gaagtgcgcg cgatgggttt tgataaacaa ccgctcgcaa agttgattcg tttacgcaat
    11041 caccagatca cgcaggactt tatcaaccgc atgcgctcgg caggattcga cggcctttca
    11101 atcgaacagt tgatccggct caagaaccac gagatcactc ctgagttcgt caacggcgtg
    11161 aaggcggagg gctacgcgga cattcccgtg gaaacggctg tcaggctcaa gaatcacgaa
    11221 gttgaccggg actacatccg gcgcgtcaag gcgcgcggct tcaccgacct cactctggag
    11281 cagatcatca agctgcgctc gcgcgagatc attaaataac ccgcgagggg tcggagccgg
    11341 ggcggctggc atagcgtcgc cccggcttta atttcacttc cgccgcaaat aatcgccaca
    11401 aacgtctccc cgtcggtgtt ctctagttga ggtctatcta cggtctgaaa atcagatgtc
    11461 aaaagtctga cctgaccaaa caccagcaac gacacttgat ttccgcttta tgaaaaacat
    11521 ctcagggctt tgccttgtta tcctcctgtg tgccttgaac tcgtcggcgc aaactctccc
    11581 tcccaatccg gctgaggccg gtgcgagcgt ggcgaacccg ctggcaagcc cgcccgcctc
    11641 gccttccctt tcggcctcga tagaggcggc aacggcaacc gcaacgccgg gcgtggaaac
    11701 gcccgtctcg ccctatgcgc gaaaggacgc gaccgtgcgg attccccgtt tcgaaagcgc
    11761 gccgttgatt gacgggcagt tgaatgatgc ggtctggcgg agcgcaaccg tcttcggaga
    11821 ttttctgcaa acgcagccgg gcgataacgt cgccccgact cacccgaccg aagccttgat
    11881 gggctacgac gccaagaatc tctatctggc ctttcgcgtc aaacaggacc gggacaaagt
    11941 gcgtgcgacg gtggcgcggc gcgacaacat tttcaacgac gattacgtgc tcgtctacat
    12001 agacactttc aacgaccagc gacaggctta cgtgatcttc ttcaacccgc tcggcataca
    12061 ggccgacggc acctacaccg aagggcgcgg cgaggactac agcgtcgatc tgttgatgga
    12121 atcgaaaggc gtgctgaccg aagacggctt tacgattgaa gcggcgattc ccttcaaatc
    12181 gctgcgctac gaggcgggaa aaaataagca gtggggactg cacatcttcc gccgcgtcaa
    12241 atacaacaac aacgaataca actcctggat gccgaacaac cgcagcatct ccggttcgct
    12301 caatcaagcc ggacgcatca cgggtctgga agacatctct accacgcgcc agcttgagtt
    12361 taatccgacc ctcacgcttt cagaatccgg caggcgcacg cgttttactt tcgaccagaa
    12421 tccggcgggg cgtttcgtca acgaaggact caaacccgaa ctcggcatga ccgcgaagtt
    12481 cagcctgagt ccaaccgtga cgctcgactt cgcctacaac cctgacttcg cacaggtcga
    12541 agccgacgca cccgtcacca cggcgaacgt ccgtttcccg atcttcttcg ccgaaaaacg
    12601 ccccttcttt ctggaaagaa tcgatatttt ccagagcggg ctgaacatcg tcaatacgcg
    12661 cgcgattgcc gaccctgacg tggctctgaa attaaccggc aggcgcggtc ggaatacgtt
    12721 cggcctgctc ttcgcatccg ataacgcccc cggaaactac tcgaaagacg agcggcaggg
    12781 tctggacttg tgccgcgctg ctaatcccgg caaccagtgc ggcatagagc ctttcgtgga
    12841 taagaactcg aacatcggcg tcgtgcgact caaacgcgac atcggcaggc agcacaacct
    12901 cggatttttc gccaccacct acagcttccc cgaacgccac aatcacaccg ctgggttcga
    12961 cgggcgtttc cgcctcgatg agaagaccgt cgcagagttt cacgtcaccg gcacaaactc
    13021 gcgcggccat ttttacgacc cggacatgga cgggcatctc tatcgaaccg gcaacgggtt
    13081 tggctatagg gcttatctcg aaaggtctga cagaaactgg tacatgaatt atctcgccat
    13141 tggcagaaca cgcgactacc gcgccgacgt gggatttaca cagagagtgg acaccaacta
    13201 catcggctct ttcattcaat atcagaccga ccgcgacgcc aagaaaagca tcatctacag
    13261 acgcctgaac aacgcgacca acgccactta tgactggaag ggtcgcacgc agggtttgca
    13321 atcgaacacg cagggaatgc tcgccctgca aagacagatt ttcgtcggcc tcggattgga
    13381 gtatggctac gagcgcgtgt tcgagcacga gtttggcgcg aggcgtacgg cgacgagggc
    13441 aggcaggttc ttcggagaga gttccgaacg ttcctcacat cgcggcgaaa tctatggttt
    13501 tatcgaagcg acgcccgtga aacaactgtt tatctacacc gtgctgagtc atagcgacgg
    13561 aaatctcgat tacgatctcg gcgcaggtcc aaaatacccg cgcgtcagcc cgccctatct
    13621 ggcctttgtc gaagagaacg agcggcgcaa acgagagggg cgtgtggatt tactgccgag
    13681 gccgggactc gatccgggcg cgggcaatca actgttcatc gaatcttcaa ttcgttacca
    13741 accgaccgtc gctctgcagg cgcagttgaa ttacacacgc acgcgtctca cgcgtcacga
    13801 cacaggccgg gtggcgtttg acgacaacgt tttcagcctg cgctcgactt atcaattcac
    13861 gcgcgacact tttgcccgcc tgcgtctcga ttactccaac atctccacgc ggtttcgacc
    13921 gcagttgatc ttgggctgga cgccaagccc cggcacagcc ctctacgcag gctacaacga
    13981 cgacctgaac tatcacggct acaatcccta cacgggagtt cacgagccgg gctttcgcgg
    14041 aaacggacgc accttcttta tcaaggcttc ctatcttttc cgcaagagtt tttagattga
    14101 taccgaagca gcctgcgcgc gtttttggga cggagccggt gcgttctgtc ctgtgttatc
    14161 ggacactgtg caggcggctt ccctgctcgc agtgccagac caactcagtt gcgccgctgt
    14221 taggcgttgt agagatcgtc gttgaagacg taggccggat ccgccgctgt cgaggatgaa
    14281 gtcttcgccg ggcgtgctga gcgtctttca ctcctcccct atcctttcag tcttcagtcc
    14341 tgcgtgtttg cgcagtaatc gtcgcggcgt cgccgctttt tccggcgacg cgtagaggct
    14401 gttcgataag aagtacctga agtttatcgt cgccgccttc ggtttcggcg ggcacggatg
    14461 tcgccttgcg gtgcgcctta aaatcggtgt tcctcttcca tgcgcggggg actttgggga
    14521 tgccgacggg atagatgacg agtgcgccgt cctgcccgat gtgcaggcgc aggaaatgct
    14581 tccagtcctc gattttgagg gaactgaaag cctcgttata gtggcgaccg aaaatgttca
    14641 tcgacaccca caggtagatg cccatgatga aagacccgac gatccagccg ccgacaaaga
    14701 tgatggccgc catcatcagg tggagtagta agagcttaat gatccccggc gacacttgtg
    14761 cgccgaggcc gagacgcgac gctgtccagt aagccaagac gcagagcagg atgagtgcga
    14821 cgacatgcgt gatgccgtgc gtcagccccg ccagccagcg ataccacttc gaatgcgtgt
    14881 cggtgaagag cagaaagccg gtaataatga gtgcgagcca aaaggtcaat ccggcgtcgg
    14941 ggaactggct tgcgacgacg tgtgaaagag tctcccagaa gttccgtgtc gagtcggcgg
    15001 cttgagcttt gaaaaactcc tgcgtgtaag ggacgacgac ccacgaggag agccagtaga
    15061 ggattgccgt caggagtccg aagaaagggt tgtagatgag gaagaagagg ttgcggcggc
    15121 aagccttttt cgactcgtcc cattcgggaa acgacttctc caacctgaag actctcacct
    15181 tcggcgtgtg tccctccgtc ccggttttga actttcgctc ggctctctct tcgcgcgaca
    15241 tcacagagtc cgtaaccgtc tccctctccg ggtcgtgctc ggggttgatc cgtcccatac
    15301 ggtgcgtcgg gtggaggaac gcgccgccgc cgcccgccgt aatcttctga atcttttcgc
    15361 cgtgctttgt gacgtgctcg tggcggcgat agtgatgcag atcgcccgag agatagacgt
    15421 gcacttccgc cttatcccct agaatttttt cctcgaagaa tttgagattg ctgtcgttgt
    15481 attgctcgtc atatttttga taaaggtcgc cgagcaccca gtgcggttcc gccgtgcaga
    15541 ggatcacgcg gtcgccttcc tccatctgct taaccacctt ttcaaaaaaa cgaacctgcg
    15601 cgccgtcgag atcggagccg agttgcacgt cgatcccgat gagccaccag ttatgcggca
    15661 gacgcagcgc gtagtagctg cgtgtttgcc gggtcagcca accggcgaac caacgccgtg
    15721 cgcaaaacag ccgggtgaag gagacgagac tgtcgtacca atcgtgattg ccgggcgtgg
    15781 cgaagagaaa cggatgcggg tcggtctgtc cggaggtgtc gccgggggcg gtgctgccga
    15841 gcgcagcctg ataaggcttg atcagacgat tcgcatacgc cttacggctc gccaccgggt
    15901 agacctgatc gccgccgaag acgagtagat ggccgcgccg cgtgaccaac tctttctcgg
    15961 aattatcgac cttgaatttc aactcatctt tagtcgccgt gtaggcgacc gcgtaggtcg
    16021 agttccaacc gtcgcccgtg tcggcgatat agtcgatcca tatctcttcg cgcttccggc
    16081 gctcgtcctt ctcgtattcg ccgtcttcgt ctctgacata ccaattggcg ttttcgttat
    16141 ccctgatgtc cccttcgtgt ctgactttgt agtgatgcgt gtagtcgtaa aagacttcac
    16201 tcttcttttc ggaatcggtg gcgagggctt ccagcaagcg atagtcggaa tggcgtccga
    16261 agatggtcga gacaagcgtg tcaaaggcgg tgcgcaccag tacagtcggt ctgtaccaac
    16321 tgaccatgcc catcgcatgg ggaatttcta tgtctgacgg tttactggac aactcgctgt
    16381 tccggtcctt cataagcggt tctcttactt tccgaggaat tgagtttgcg ggagaatcga
    16441 gccaacagca ggccggtttt taacgaatgc ggttgctacc gacatgaaca cgcgccttcg
    16501 tcttcgaaga ccagacattt cgacgataca tacccgactg ttcagcccaa gcgaccgtct
    16561 cgctctaatt ctgcttaccg ggtacgtgag attaacaatg aaagacgcat taccttaatt
    16621 gctggcacaa aacttcggac tcatcctatt cttcacgctt agctattcat attcaattat
    16681 ttagacgttg tgaaacaacg tgaaacgccg ccctctctct tctttgcgtc gcttatcgag
    16741 taagacaata gagaagcata ttgagtagcc tccgcatgaa acttacttct cttccgtctg
    16801 ggtgttgatg agtttcgtgt gtctgcactt ggctacgcct ttatctgtcc cttctggata
    16861 ccagaagtta gtgcctgcac agtccgggca gcgtcgtttc tcttcttcgc tgagcgtggg
    16921 ctggattccc tgctcttctg cttcgacggc aatctctgag gcgcgcttct tatccagctt
    16981 ccagagtctg cgccgcaggt gctcggtgag gaaagccggc acgttcgaga cggtcgtgcg
    17041 tccagccgca atcttgagtt cggtgacgag aagttcggcc agttccgccc attgcgcggc
    17101 attggcagag ttcttacccg tcagctcttt ttccgcttgc aggaagagtt cgaccagccg
    17161 gcgcgcagcc tcatcatcaa tgctctcttc tttagtctta aaagaagtct taagttcccc
    17221 agaaaccgtt gaatctgcgg cacttagact caccccactc accccacttt ctgcagtgag
    17281 tggtgtcacc actttctgca gtgagtgggg tgagtgggtg gggtgagtgg gtggggtgag
    17341 tggggcgacc tcttccggta gtataatagt gtactcactg ccgtcctgtt caccagcgat
    17401 agagcgtatc tgcaacagcc cagccgcttc gagtcggcgg agatttttat acatcgttcg
    17461 ctctgagccg atatgcgagc ctttcatgat ggcagtcttg gtcatgcgca cggaacgctt
    17521 gggaactacc gcacccctgg ttttcgcata gaggtaatcg tatagctgtt tgcttttgcc
    17581 agtaaacgcg cccgaaggca cggcttcgcg cacgattgaa ttggcaactc tggcaaagtc
    17641 acgagcgggc gcgatggctt gtggggtggg tggggtgagt ggggtgagtg gagtggccgg
    17701 ggtgggctgg gtcaatccca ataactcttc gaccggatga ggtttgcgca ggttggcgaa
    17761 ggtatcttgt gtcttcttct gaggttgtcg agtcttgatc tcagtcttct gtttgccttt
    17821 ggtttccgtc atccggcctc ctgaacaagt ctgagggaac tatccatttt tagtctttgc
    17881 agcacttcgt ccgtcaggga gtcatagtct ttactgccgt gcgaatcggg cgcatgctcg
    17941 aagatgcttt tacgaaggga aggcgcggtt tgcagtttga cgttcgttct gatgaccgtc
    18001 tcgaacacct ggccgtcgaa catttcctgc attttagcta aagcctcacc acagatgcga
    18061 ttgcgtccgt cgaagtaggt catgaggtag cccagaatcg agagggaagg attgagcctg
    18121 cgtgtggcct gcatcgtccc tgtcaagtca accacgcctt cgagcgggta gaactgcgcg
    18181 gcgacaggaa ctatcgcgtg agaagaggca agtagcgcct gcgtcaaggt cattcccaga
    18241 ctgggcgggc aatcaatgac ggcgaagtcg taggcttcca gtgattccag agaatctttg
    18301 agcctgtagt gttcctcgat ctgcacggag cgttccaaga gcgcgagcct gatgtgagag
    18361 ggcgcgatgt ctaaaccttc gatgtgcgtc tcgtagatcg cgtcaatcaa actcgggcgt
    18421 tcctggtagc cgaccaacac gtcggccagc gttgtcttaa tgaggttctg gtcgaggaat
    18481 gtgaaggtgg cgttgcactg gctatccaga tcaatcagaa gcgtcttgta gcccctgata
    18541 gctagcccgg cggcgaggtt ggtagccgtg gtcgttttcc ccacgccgcc cttctgattt
    18601 gcgatggcga tggtcttcat gccgcgatac ctctccttac cctgtcagca tcgtcgagca
    18661 ctctctgtat gtcgctgacg cgaacccttt tgactcgcgc gtcgagcacg tcctgaaagg
    18721 tttcgatatt gtaggaacga atcagacgcc acagcgccgt gcgcgaaact cctagtcggg
    18781 tttgcgcttc gctgatgctg atagtttggg gcgtggggtt ggttttgatc atagtctatg
    18841 cctcgtgctt gataacgacc gtttgtgtct tttatcgcgt gtagcgcacg atgtcaatcg
    18901 aaatgtttca gcgcggtgaa tggagaggga ctatttagcg tggtcgaata cggcgcgctt
    18961 ccgctttcag gagtggcttt cgactataag ccactcctgc aacgcaaacg ccccggaaca
    19021 ggattccgag tagctgccac gtgtttatgg gtttccgaat ctcaggcggt actctgtgga
    19081 gttgacgaag ccgttgacca tgatgcggat gttgttcggg tcttcggtga tgactctgag
    19141 ccaatcctga tagcccctct cctccggcgt ccggcgcagg tagccataat actgcatcgc
    19201 cacaaacgct cccttgtact catgtgcggc gatctcttcg gactcgacaa tcgcgcgcaa
    19261 aacttgcgct ctggtcagag cctgcgcgcc tgtcgtgccc agacggttaa ttaaatccgc
    19321 gcgtgtcagg gtcaacagcg tctcaccctc cgggttttgc gggtcgggcg tggtgatctg
    19381 ttgcaaaccg tagcggtcga gcagcgcgtt gacataggcc gtgttggaga gcgagtcata
    19441 agcggccttg aacgccggtc gctgtacgaa ggcgacgggg aaggcagcgc ggcgggcata
    19501 ggtgtcggca gaagtttgcc ctgtgaccga gcgcatgtcg ggaatgatct ccgcatactc
    19561 cgggcggcgg ttcaaggcca cgcggtagaa gttatagacg aaaaatccct tcaacctgaa
    19621 ctcgcgcgag ccgaagaaac cctgcgagac ggtgattcga tcacacgccg gattgttgtt
    19681 aacgttcgga cacccgttca agaccgcaga ccaaggctcg ccgggttccg gttcgcgtga
    19741 gaggaagtct agatagtgca tcctgacgaa gaaggtcgtg ttgaagatgg ggttggctcc
    19801 cgtcgtggtg tcgttgtccg agatggtgag cgtcgcggtg ctttgagcgc cgagcgcggc
    19861 caatccggtc gcgtcgctga gcctgagtcg cactgtctcg ttgccctcga catgcgcgtc
    19921 gtccacgagc gggatgctga tctgtttgct ctcttcgccc ggcgcgaagg tcacagtgtc
    19981 aacggtcgtg gcgtagtcgc agcgcgcgta ggccgtgccg ttcgccgtgg cacaggagac
    20041 ggcggcgggg tcgtccacag tctcgaattt aaccgccacg ctgaatgaac tatcacccgc
    20101 gcgcgtgatg actgcgacgg cgcgacccgc gccctccgag aacgcgtagg cgggctggct
    20161 gaactgtagc gtcggcactt ccggaattac ctccggctgc gtgatggtga gcgtctgccc
    20221 ggcgatgaag acggtgcccg tgcgtggcac tccagagccg ttcgaagaga cggagagcgt
    20281 aacggttccg ctgcccgtgc cgctcgcgcc ggagcttacc gtaataaagc cgctgttgct
    20341 gaccgccgcc cacccgcagc cgccctgcgt cgtcacgttc accgcgagcg tctcgccgtt
    20401 ggccggagag gtgcgagtcg cgggactgat cgtgtaggag caggagttga cgagacggtg
    20461 gacggttccg cccagaccga cgacgtaaag ttcgcccgtc tcatcttcgc cgaatgaaga
    20521 gatgttgatg tttgtgtcct ggaggaagga ctgcgccgtg ccctgtagca ggaacatttc
    20581 tccggtgcag aagtctgcgt agatgtatgc gccggtgggg atagtcgcgc gcgtgccgcg
    20641 atagacgtag ccgcccgtga ccgagcaacg cccgcccgta tgatcatact cggcgacagg
    20701 cgactgaaag ttagcggcgt tgcactgcgc aggtccgagt ccggtgcatc tggtgccttc
    20761 gaaaacacgc cagccgtaat tgccgccgcg cgtgatgatg tcgatctctt cgcgcacgcc
    20821 ctgccccacg tcgcccgcgt agagttggcc ggtggcgcgg tcgaaggaga aacggaaggg
    20881 gttgcgcagg ccgactgcgt aaatctcgtc gcgccccggc gtcgcgccaa agaaagggtt
    20941 gtcgggcggc gaggaataga gcgtggagcc gctggcttga tccacgttga tgcgcagcat
    21001 cttgcccagc agttcctcca cgttctgcgc gcgattctgc gggtcgttgg ccgagccgcc
    21061 gtcgcccatc ccgatgtaga gaagattgtc cgccccgaac gcgatcatgc cgccgttatg
    21121 attcgcaaag ggctgcggga tgacgagcag caccgtctca gcggcttcga ccgcgttggg
    21181 gttcgtcgcc gacgcgcgat actcggcgat gacggttgcg ccgtcaggct gccgcgtgta
    21241 gttcacaaac agacgccggt tggcgcgaaa ctgcggatgg aaggcgagtc cgagcaaccc
    21301 gcgctcgccg cctgagagca ccttcgccgt aagatcgaga aacacagacg gcgtggaagc
    21361 tcccggttgc agcacctgta cgcgccccgt ctgttcgacg acgaacaggc ggttgctgcc
    21421 atcgcgtgcg ttggtcacca gcaccgggct ggagagattt gaaagcacgg gttccagcgt
    21481 caagggggcg acgccttgcg ctgccgcgct ggcgcacatg aaggcgaggg cgagaaaaag
    21541 cgtagcgacg aagcggcgcg ttgacctcat ttctgtacta cctcctgctt gattcagatc
    21601 acatgttgga aaggtttgtt ctgttgcagg cggaaggcgt ccgcaactcg ccggaggggg
    21661 atcggttcag gcgcattata ctgcgcgcga aagtttcgca ccatcaaaca cttcatacag
    21721 aatttcggac gcttcaagta atcagactga tctgtaacct tctgccgcga gtcagtgtct
    21781 gacacaacag ggcaactctc ctaccagacg cgacgggtgc gttatggtcg cgcccgttta
    21841 tttttcgata gggagaaaag atcatgagga aattactggc actgacattg tcactggctt
    21901 cgataggttt cgtggcttca ccggcagagg cgaaagcgtc cgccgcaaac atcgctccgg
    21961 cggctgccaa cgcggcggca gcgtcgcccg caagcgttca gtgggggcgc aataatcgtc
    22021 gcatcaaccg ccgtgcccgc accgtaacgc agtcgcgtct ggtgcgaatc ggacgacgaa
    22081 ctttccgtga gacttatcag atcacgtact ggccgaatgg cagaaccgag acgagactga
    22141 tcagccgcgt gcgagtccgt taaacaggac ggcaaaaatc gagagcgggt gcatcaagag
    22201 taaattactc ttgatgcacc cgctcttagt ttgcaaaatc gggcgttgat tccgcgtcta
    22261 aagttccaga tattttgtcc gggttcaggc cgggcggaag cctcgaatat cccggcacgg
    22321 cttaacgaag gtcactaatc gccggatgat ttgccgg
//
LOCUS       DASWNS010000639         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_206655, whole genome shotgun sequence.
ACCESSION   DASWNS010000639 DASWNS010000000
VERSION     DASWNS010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206655"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..258
                     /locus_tag="VGB76_22210"
                     /pseudo
     CDS             28..258
                     /locus_tag="VGB76_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(414..>2625)
                     /locus_tag="VGB76_22215"
     CDS             complement(414..>2625)
                     /locus_tag="VGB76_22215"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0101657.1"
                     /translation="SRIPVNGFIILPFSGTYSILATSFAPEARGAYTLTLTAGANCSL
                     TPIAFGETRQGTLSAGDCTNAIDLDGTLVDLYTFNGTAGQQVSITMTATAPGNVDPYL
                     YLLLPNGDLSAENDNGGGGTAARIPPNGGFGRLPITGTYTIVANTLATNQSGNYSLTL
                     TKAATDCASVPLTPGTTVNGTLANGDCRLLEDGSFLDAYTFNGAANAQITLTMTSSTA
                     GLSPVVFLLSPTGYVLAIGTPPTGGSPARIPDTGSFTLPTTGTYTVLANSSVAGQTGD
                     YTLALTGGVQQNTHTISGQIRSGVDQSSVGLSGITVILSGGASATTTTDGSGHYSFAG
                     LPAGNNYTITPTNTAQHSFTPQTVIALQADQMVNFIGIPHVIISEFRFRGTAGATDEF
                     VELYNQTDQTRDITGWSLVSGNTVLHTVSSGSIPARSHYLVTGANYSLPAASDGALNV
                     DIPDSAAVALFTNAPTFTSGSRIDAAGFSTAVTLYTEGGGLSPASGITTDSEHAFVRK
                     LTSGTPQDTDNNSTDFVLVATDPLVVGNNAVLGAPGAENRTSPIQRNATIKGSLIDPQ
                     CAGFGAATSGCARVRTAAGANPTTAQYGTLRIRRKFTNTIGANVTTLRFRVVGITTQG
                     SPGAGSGQADLRVLSSSPADFNVTLTNGAIVPVKGTQVETPPAQPNGGGLNSTVITIT
                     TATPVLPNNSVNVEFTLGVQQEGAFSFFVNVEALTALEATKAGVMKRAGSRKAK"
BASE COUNT          486 a          716 c          901 g          522 t
ORIGIN      
        1 cgccgtagat aaagaagggt aagccattat ctgaacaatg tagtcgaaca ggatcgtcgt
       61 ttcatcaaga agaaagtgcg ggcatcacaa ggcttcaaaa gatttcacac agcagagcga
      121 actccggaag gcattgaggc gatgcacatg tcgaggaagg ggcaagtcaa aagactaagt
      181 ggtaatgata gcgcaggggc gggtgaagtt tgtcgctccg ctcttccgga tcgccgcctg
      241 agaaggagtc gccgatgacc ttctgcgttt gaaaatgatt tttgcaacac agccgggaat
      301 gaacccggcc cgcatacgaa gaggccgccg gagccgcaag gtctctggtg gcctcttcgc
      361 tttcacccgt tagctctcct gtcggggagc aggcgaatcc ggttagctgt gctttacttc
      421 gctttgcgcg agccggcacg cttcatcacg ccagcctttg ttgcttcgag tgcggtcaac
      481 gcctcgacgt tgacgaagaa gctgaaagcc ccttcctgct gcaccccgag cgtgaactcg
      541 acgttgaccg agttgttcgg cagaaccggc gtcgccgtcg tgatggtgat gacggtggag
      601 ttgagtcccc cgccgttggg ctgcgcggga ggcgtctcaa cctgagtgcc cttgacgggc
      661 acgatcgctc cgttggtgag cgtgacgtta aagtcggcgg gcgaagacga gaggacgcgc
      721 agatcggcct gcccgctccc cgcaccggga ctgccctgcg tcgtgatgcc gacaacgcgg
      781 aagcgcagcg tcgtcacgtt cgcgccgata gtgttggtga acttgcggcg gatgcgcagc
      841 gtgccgtact gcgcggtcgt cgggttagcc cccgccgccg tgcgcacgcg ggcgcacccg
      901 ctcgtcgctg ccccgaaccc ggcgcactgc ggatcgatga gcgagccttt gatcgtagca
      961 ttgcgctgga tgggactcgt ccggttctcc gcgccgggcg cgccgaggac ggcgttattg
     1021 ccgactacaa gcgggtcggt ggcgacgagc acgaagtcgg tggagttgtt atctgtgtcc
     1081 tgcggggtac cactcgtcag cttgcgcaca aaggcgtgtt cgctgtcggt tgtgatacca
     1141 ctggcgggcg agaggccgcc tccctcagtg tagagcgtga ccgctgtgct gaagccggca
     1201 gcgtcaatgc gcgagccgga ggtaaaggta ggcgcgttgg tgaacagggc aacggcggcg
     1261 ctgtcaggga tgtccacatt gagcgcgccg tcggaggccg caggcaggct gtaatttgcg
     1321 cccgtgacca gataatgcga acgcgcgggg atcgaaccgc tgctgaccgt atggagtacc
     1381 gtgttgccgg agacgagcga ccaaccagtg atgtccctag tctgatcggt ctggttgtaa
     1441 agttcaacaa actcgtccgt tgccccggca gttccacgga agcggaactc gctgatgatg
     1501 acgtgcggga tgccgataaa atttaccatc tggtcggctt ggagcgcgat tacggtctgg
     1561 ggggtgaaac tgtgctgcgc agtattggtc ggcgtgatgg tgtagttgtt gcccgcggga
     1621 aggccggcaa atgagtagtg accgctccca tcggtcgtcg tcgtggctga tgcccccccg
     1681 cttaaaatca cggtgatgcc gctcagtccg acgctgctct ggtcgacgcc gcttcttatc
     1741 tgaccgctga tagtatgagt gttctgctgc acacccccgg tcagcgcgag ggtgtaatcc
     1801 cctgtctgtc cggcgacgga ggaattagcg agcaccgtgt aagtgcccgt cgtgggcagg
     1861 gtgaatgaac ccgtgtcggg gatgcgcgcg gggctgccgc ccgtaggcgg tgtcccaatc
     1921 gcaagcacgt agccggtggg cgagagcaga aacacaacag gggataaccc ggctgtggag
     1981 gaggtcatcg tgagggtaat ctgcgcgttg gccgcgccgt tgaacgtgta agcgtccaga
     2041 aaggaaccgt cttcgagcag gcggcaatcg ccgttggcga gcgtgccgtt gactgtagtg
     2101 ccgggagtga gcgggacgct cgcgcaatcg gttgccgcct tcgtcagggt gagactgtag
     2161 ttgccgctct gatttgtcgc tagcgtgttg gcgacgatag tatatgtgcc tgtgatgggg
     2221 agtctgccga agccgccgtt gggcggaata cgcgccgccg tgccgccgcc gccgttgtcg
     2281 ttctcggcgc tcaggtcgcc gtttggcaga aggaggtaga gataaggatc gacattgccg
     2341 ggggcggtgg cggtcatcgt gatggagact tgctgccccg ccgtgccatt gaaggtgtag
     2401 agatcaacca gcgtgccgtc gaggtcgatg gcgtttgtgc agtcgcccgc gctgagcgtg
     2461 ccctgtctgg tttcgccaaa cgcaatgggc gtcaaactac agtttgcgcc agccgtcagc
     2521 gtgagggtat aagccccgcg cgcttccggt gcgaatgaag tggcgagaat cgaataggtg
     2581 ccgctgaagg gcaggatgat gaaaccgtta acggggatgc gcgaa
//
LOCUS       DASWNS010000640         5250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_334566, whole genome shotgun sequence.
ACCESSION   DASWNS010000640 DASWNS010000000
VERSION     DASWNS010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334566"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /gene="boxB"
                     /locus_tag="VGB76_22220"
     CDS             complement(<1..1293)
                     /gene="boxB"
                     /locus_tag="VGB76_22220"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEY0101658.1"
                     /translation="MSMNLYERIPNNVNLSEDKRLQRALEKWLPNYLDWWREMGPDGF
                     QDKEVYLRTAISVDADGWANFDYVKMPDYRWGIFLKPADANAKVGFGDNYGMPVYQEV
                     PGEFRNALRRIIVTQGDTEPASVEQQRELGKTCPSLYDLRNLFQVNVEEGRHLWAMVY
                     LLQRYFGQDGRDEADELLQRRSGNPDKPRILEAFNQPCDDWLNFFCFTMFTDRDGKFQ
                     LAALAESGFEPLARTCQFMLTEEAHHLFVGETGVGRVVKRTAQLMRENPDVDVRELGA
                     IPLDILQRYINFWFSYSLDLFGGEISSNSADFFAAGLKGRFKEQDNYAEHTATNENFM
                     LTVVEDGRLKQREVPLRNALNEVLRGEYVKDCERAMTRWNKFLRDEGIDAALYLPSTR
                     FHRHVGEFAGHTFDVEGNLISAEEFERRRAEWLPSLADR"
     gene            complement(1396..3027)
                     /gene="boxC"
                     /locus_tag="VGB76_22225"
     CDS             complement(1396..3027)
                     /gene="boxC"
                     /locus_tag="VGB76_22225"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEY0101659.1"
                     /translation="MINFETTPESYKHWKLAIDGAIATLTMDVQEDETLAEGYKLKLN
                     SYDLGVDIELADAVRRLRFEHPEVQVVVVTSLKPRIFCAGANIYMLGTSSHAFKVNFC
                     KFTNETRCAIEDASTNSGQRYVAALNGTASGGGYELAIACDEIYLVDDGNSAVSLPEV
                     PLLGVLPGTGGLTRLVDKRKIRRDRADVFSTLAEGLKGKRAKEWGLIDGYFPTSKFQD
                     AIDAHVRSLVKTDGAKAKAVGIKLNPLQVEATGDGREYKYVSVKLNREKRYADLTMRA
                     PQGELPTSIEEIEKLGDAYWPLQAYRELDDALLHLRMNELEIGLVCLRTEGRIADVLA
                     VDQTLAAHKEHWLVREIILHMARVLRRLDLTAKSFFALIEAGSCFAGNLLELVLSADR
                     TYMLNDPEVRVEVASSDFNAGALPMSNGLSRLQSRFLAAPEQAAAVLAHEGAFDAEAA
                     EEAGLITFAPDDLDWEDKIRVDIEERTSLSPDALTGMEASLRFAGPETMDTKIYGRLT
                     AWQNWIFQRPNAVGPEGALTNYGKPTQAQFDYKRT"
     gene            3355..3543
                     /locus_tag="VGB76_22230"
     CDS             3355..3543
                     /locus_tag="VGB76_22230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101660.1"
                     /translation="MPIKETERREISPKTPIPAPDPQAVCKGYGACSKSGCNCQAYEG
                     SGSTCGNQGCGHAYQDHW"
     gene            3709..3807
                     /locus_tag="VGB76_22235"
     tRNA            3709..3807
                     /locus_tag="VGB76_22235"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3743..3745,aa:Sec,seq:tca)
     gene            3912..5039
                     /gene="dprA"
                     /locus_tag="VGB76_22240"
     CDS             3912..5039
                     /gene="dprA"
                     /locus_tag="VGB76_22240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY0101661.1"
                     /translation="MKDWIALNMTPGVGPRAAARLLERFGSAEAVFAALRAELERLRL
                     RPEAVESIVLRDRHAEAGRELERVRALGADVLILDDGTYPALLREIADPPITLYVRGE
                     WESALDAPCVAIVGSRRCSTYGQNVALMLARDLAARGVTIISGLARGIDAAAHRGALE
                     AGGRTVGVLGTGVDEVYPRDHRKLAEEILARSGALVSQFPLGTPPIPENFPYRNRIIS
                     GLSLGVVLVEAAENSGSLITARLAMEQNREVYAVPGNVTSRNSFGTNYLIKGAGAKLV
                     QQWQDVAAELPPDIAAQLLPPEPAKKKAGATQQQPALPADLSEGERAVMKLLSPDEAV
                     HIDALIGASNLSVSVLTGILLGLEMRELIRQLPGKCFVRKL"
BASE COUNT          962 a         1549 c         1665 g         1074 t
ORIGIN      
        1 gcggtcggcg agcgagggca gccactcggc gcgtcggcgt tcaaactcct ccgcgctgat
       61 gagattgcct tcaacgtcga aggtgtgccc ggcgaactcg ccgacgtggc ggtggaagcg
      121 cgtgctcggc aggtagagcg cggcgtcaat gccttcgtct ctcaggaatt tgttccagcg
      181 cgtcatcgcg cgttcgcagt ctttgacata ctcgccgcgc aggacttcgt tgagcgcgtt
      241 tcgcagcggc acttcgcgct gtttgagccg cccgtcttcg acgacggtga gcatgaagtt
      301 ctcattcgtg gcggtgtgct cggcgtagtt gtcctgctcc ttgaagcggc ctttgagtcc
      361 ggcggcgaaa aagtctgcgg agttggaact gatctcgccg ccgaagagat cgagcgagta
      421 ggagaaccag aagttgatat agcgttgcag gatgtcgagc gggatcgcgc cgagttcgcg
      481 cacgtccacg tccgggttct cgcgcatcaa ctgcgcggtg cgtttgacga cgcgaccgac
      541 gcccgtttcg ccgacgaaga gatgatgcgc ttcttccgtg agcatgaact ggcaggtgcg
      601 cgcgagcggc tcgaagccgg attcggcgag cgcggcgagt tggaatttgc cgtcgcggtc
      661 tgtgaacatc gtgaagcaga aaaagttgag ccagtcgtcg cagggctggt tgaaggcttc
      721 gaggatacgc ggcttgtcgg ggttgccgct gcgccgttgc agcagttcat ccgcttcgtc
      781 gcgcccgtcc tgcccgaagt agcgttggag caggtagacc atcgcccaga ggtggcggcc
      841 ttcttcgacg ttcacctgaa agagattgcg cagatcataa agcgaagggc acgtcttgcc
      901 gagttcgcgc tgctgttcga cggaggcggg ttcggtgtcg ccctgcgtga cgatgatgcg
      961 gcgcagggcg ttgcgaaatt cgccgggcac ttcctgatag acgggcatcc cgtagttgtc
     1021 gccgaagccg accttcgcgt tcgcgtcggc gggcttcaga aagatgcccc agcgataatc
     1081 gggcatcttc acgtagtcga agtttgccca gccgtcagcg tcaacgctga tggccgtgcg
     1141 caggtaaact tccttgtcct gaaaaccgtc cggtcccatc tcgcgccacc agtcgagata
     1201 attgggcagc cacttctcca acgcccgctg gagccgcttg tcctccgaaa gattaacgtt
     1261 gttcggaatg cgttcgtata aattcatgct catggattaa ccctctgtga caggtgacga
     1321 gtgacaagtg atgagttgaa gacaagaaaa caagctttca gtttttaact tgtcacctgt
     1381 cacccgtcac tgcttttaag tcctcttgta atcgaattgc gcctgcgtcg gtttgccgta
     1441 gttcgtgagc gcgccttcgg ggccgacggc gttgggacgc tggaaaatcc agttctgcca
     1501 cgcggtcagg cgtccgtaaa ttttggtgtc catcgtctcg ggtccagcga agcgaagcga
     1561 tgcttccatg cccgtcaggg cgtccggcga gagcgacgtg cgctcttcaa tgtccacgcg
     1621 gattttgtct tcccagtcga gatcgtcggg cgcgaaggtg atcagcccgg cttcctctgc
     1681 cgcttcggcg tcgaacgcgc cttcgtgcgc gagcaccgcc gccgcctgtt ccggcgcggc
     1741 aaggaaacgc gattgcagcc gcgacagccc gttcgacatc gggagcgcgc ccgcgttgaa
     1801 atcgctcgac gccacttcga cacgcacttc cgggtcgttc aacatgtagg tgcggtctgc
     1861 cgagagcacg agttcgagca ggtttccggc gaagcaactg cccgcttcga tgagcgcgaa
     1921 gaaactcttc gccgtcaggt cgagacggcg cagcacgcgc gccatgtgca ggatgatctc
     1981 acgcaccagc cagtgctcct tgtgcgcggc cagagtttga tcgacggcga gcacgtccgc
     2041 gatccggcct tccgtgcgca ggcagacgag gccgatctcc aattcgttca tgcgcaggtg
     2101 caggagcgcg tcgtcgagtt cacgataggc ttgcagcggc cagtaggcgt cgccgagttt
     2161 ttcaatctct tcgatgctcg tcggcagttc gccctgcggc gcgcgcatcg tcaaatccgc
     2221 gtaacgcttc tcgcggttca atttcacgct gacgtatttg tactcgcgcc cgtcgcccgt
     2281 cgcttcaact tgcaaagggt tgagcttgat gcccacggcc ttcgccttcg cgccgtcggt
     2341 tttaaccaga ctccgcacgt gcgcatcaat cgcatcctga aacttgctcg tcgggaaata
     2401 gccgtcaatc aagccccact ctttggcgcg cttgcccttg agtccctcgg cgagcgtgga
     2461 gaagacatcg gcgcggtcgc gtcgaatctt gcgcttgtct acaaggcgcg tgagtccacc
     2521 cgtgccgggc agcacgccga gcagcggaac ttcgggcagc gagacggcgg agttgccgtc
     2581 gtccacgaga tagatttcgt cgcaggcgat ggcgagttcg tagccgccgc cggaggccgt
     2641 gccgttcaga gcggcgacgt agcgttggcc ggagttggtc gaagcgtctt cgatggcgca
     2701 gcgcgtctcg ttggtgaact tgcagaagtt gaccttgaag gcgtgcgacg aagtgccgag
     2761 catgtagatg tttgcgcccg cgcaaaatat gcgcggcttg aggctcgtga cgaccacgac
     2821 ctgcacttcc gggtgctcga agcgcaacct ccggactgca tccgccagtt cgatgtcaac
     2881 tccgagatcg taagagttga gcttcaactt gtaaccttcg gccagcgtct cgtcttcctg
     2941 cacgtccatc gtcagcgtgg caatcgcgcc gtctatcgcc agtttccagt gcttgtaact
     3001 ttccggtgtc gtctcaaaat taatcatcag ataactaaac tccgactgag gtgaaggcgc
     3061 gccacaggcg cgtgcgaaaa tttatcaata tcatttttcg aacgctcgtt cgaaaaatga
     3121 tgataacggc agatgccctg cccgtcaagg cgcggaaaag gcgagggttg agcgttgaca
     3181 acccgccggc gtttgcatat cttccccgcg cccggatcga cggggcgctc cgtgcggaga
     3241 agtcttaact caccgcaagc tgttcctcct gcgagcgcac ttcgatagcg cgatgcgcaa
     3301 ggtcgcggca cgaaggcgtt cactctctga ccaactgacc gaaggagact ttctatgccg
     3361 atcaaggaaa ccgagcgacg agaaatttca cctaagactc ccatccccgc gcccgaccca
     3421 caggccgtct gcaaaggcta cggtgcgtgc tcgaaatcgg gctgcaactg ccaggcctac
     3481 gaaggcagcg gcagcacctg cggcaatcag ggctgcggcc acgcttatca ggatcactgg
     3541 tagacgtgtg ccggaagcac gcactacttt ttcggagaga ggggcagcgt caaagccgcg
     3601 cccctctttt tttgcgcgtc gcgccgccag cccgtccgcc ctccggacga tctcccttga
     3661 cacgcgccgc tccacgcgcg tagggtttca gacgatttcc caccaccggg aggcgcatct
     3721 gttctggtga acggcgcggt cttcaaaacc gtctgtgaga ctgtgagaac aggctcaggt
     3781 gggttcgatt cccacacgcc tccgccacaa aatgcagtga caagtgacga gtgacaggtg
     3841 acaagttaaa gccgcaggct tgtcttgttc cgtctcgtca cttgtcactc gtcactcgtc
     3901 acctgtcact tatgaaagat tggatcgcac tgaatatgac gccgggggtg ggtccgcgtg
     3961 cggcggcgcg tctgttggaa cgcttcgggt cggcggaagc ggttttcgct gcgttgcgcg
     4021 cggagttgga gcgtctgcgt ctgaggccgg aggcggttga gagcatcgtc ttgcgcgacc
     4081 ggcacgcgga ggccggacgc gaacttgaac gcgtgcgtgc gctcggcgcg gacgtgttga
     4141 ttctcgacga cgggacttac cccgcgctgc tgcgcgaaat cgcagacccg cccattacgc
     4201 tctacgtgcg cggcgagtgg gagtcggctc tcgatgcgcc gtgcgtggcg attgtcggct
     4261 cgcggcgttg ttcgacttac gggcagaacg tggccttgat gctggcgcgc gatctggcgg
     4321 cgcggggcgt gacgatcatt tccggactgg cgcgcggcat cgacgcggcg gcgcacaggg
     4381 gcgcgctcga agcgggcggg cgcacggtcg gagttttggg cacgggcgtc gatgaggttt
     4441 acccgcgcga tcatagaaag ttggcggagg aaattctggc gcggagcggc gcgctcgtct
     4501 cgcagtttcc gctggggacg ccgccgatcc cggaaaactt tccgtatcgc aatcgcatca
     4561 tcagcggcct gagtctgggc gtggtgctgg tggaagcagc ggagaacagc gggagcctga
     4621 tcacggcgcg gctggcgatg gagcagaacc gggaggtgta cgccgtgccg ggcaacgtca
     4681 cctcgcgcaa ctcgttcggg acgaactacc tgatcaaagg agcgggcgca aaactcgtcc
     4741 agcagtggca ggacgtggcg gcggaactcc cgcccgacat cgccgcgcag ttacttccgc
     4801 ccgaacccgc gaagaagaaa gcgggcgcga cgcaacagca gcccgcgctc cccgcagact
     4861 tatccgaagg cgaacgcgcc gtgatgaagt tgctctcgcc ggacgaagcg gtgcacatcg
     4921 atgcgctcat cggcgcgagc aatctgtccg tctcggtgtt gacgggaatc ctgcttggtc
     4981 tggagatgcg tgagttgatt cgacaactgc cgggcaagtg cttcgtgcgg aagttgtagt
     5041 agcgggtcgg ttacaaataa ggaggacgat agatacgagg cgataaactg gaaccgctcc
     5101 gctacccgac tgccgctttg cctatacgtg ttgatctacc agaatcgtgt ttccatccgg
     5161 atcggagatc ataaagctgg ccgggccggt ggagctttcg tctgcttccg agatcatctg
     5221 tacgccgtgg tctttgagtc gtcgctgcaa
//
LOCUS       DASWNS010000641         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_413985, whole genome shotgun sequence.
ACCESSION   DASWNS010000641 DASWNS010000000
VERSION     DASWNS010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413985"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VGB76_22245"
     CDS             <1..782
                     /locus_tag="VGB76_22245"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEY0101662.1"
                     /translation="AQGSMGVTVADYDHDGKLDLFITNFDDDYNTLYRNDGNGFFTDA
                     SYATKIAAVSLPYVGWGTKFFDYDSDGWVDLFVGNGHVYPQIKHFHQRNFVHRNLRNG
                     TFAEVAEQLGAPFAEKRAARGAAFGDLDNDGDVDVVVSYLGGGGQVLRNDGGNANNAL
                     LVKTVGVRSNRDGVGARVRVVAGDLVQKDEVRSGDSYISQSDLRLHFGLERRTKIDLI
                     EIRWPGGAVDKITDAKVNRIITVKEGQGIVEQKDFKTAARR"
     gene            825..2027
                     /locus_tag="VGB76_22250"
     CDS             825..2027
                     /locus_tag="VGB76_22250"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101663.1"
                     /translation="MYLAQVKFTFRRAAMLAVLLCLLLVATGASAQRKGRGGSSNSRG
                     EQLERAASLIASEQLAEAEQQLSQILKVAPTEAVALNLLGAVRAKQGKLDEAETLFTR
                     AVRSDTELAGAHMNLAYLYLLKREPEKSAAALKEVLRIEPANADAAYRLAWLLHSQGR
                     YEECIAVAEKLKGSEARSARLLGVLGDAYLRKGDKAGAVSALKRAVELSPGEPSYHFA
                     LASAWLRHPPDLQEAEDSFRKFNTLSPGDAQGQLHLGYVLLKQKKHAEAREWLERSAG
                     TGAGTPEAFYYLGLIAQAQNEDARAIELFEKSIALAPAFGHVHVALGSAYLKLKDYVR
                     AQTALETGAKLSPEDSKAHYNLALLYARLKQPERAREEMLIVERLKSEGKARDDGENE
                     TYAPPPPR"
     gene            2079..>2672
                     /locus_tag="VGB76_22255"
     CDS             2079..>2672
                     /locus_tag="VGB76_22255"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0101664.1"
                     /translation="MKDKIVISSLVFMLVLNLPFYSGARQAGTDARAAVTFAEVAPKA
                     SGISWAHNNAHTPERFLPETVGAGGAFFDYDNDGWLDIYLVNSGASDFYTPARPLKNA
                     LYRNNRDGTFTDVTDKAGVAGGKFGMGAAAADFDGDGWQDLYVTNYGADILYRNRGDG
                     TFADVTQKANLTVDGWSTCAVWFDYNADGRLDLFVSSF"
BASE COUNT          545 a          795 c          869 g          463 t
ORIGIN      
        1 cggcgcaagg ctcaatgggc gtgacggttg ccgactacga ccacgacggc aagttggact
       61 tattcatcac caacttcgac gacgactaca acacgctcta tcgcaacgac ggcaacggct
      121 tcttcacaga cgcttcttac gcgaccaaaa tcgccgccgt cagtcttcct tacgtcggct
      181 ggggaacgaa gtttttcgac tacgactcgg acggctgggt cgatctcttc gtcggcaacg
      241 gccacgtcta cccgcagatc aaacactttc accagcgcaa cttcgtgcac cggaacctgc
      301 gcaacgggac gttcgcggaa gtggccgaac aactcggcgc gcccttcgcg gagaaacgcg
      361 cggcgcgcgg cgcggccttc ggcgacctcg acaacgacgg cgacgtggat gtcgtcgtca
      421 gctacctcgg cggcggcggt caggtgttgc gtaacgacgg cggcaacgcc aacaacgcgc
      481 tgctcgtcaa gaccgtgggc gtgcggagca accgcgacgg cgtgggtgcg cgcgtgcgcg
      541 tcgtcgcggg cgacctcgtg cagaaggacg aggtgcgcag cggcgacagc tacatctcgc
      601 agagcgacct gcgtttgcac ttcggccttg agcggcggac gaagatcgac ctcatcgaga
      661 tacgctggcc gggcggcgca gttgataaga tcaccgatgc gaaggtgaac aggataatca
      721 cggtcaagga aggtcagggc atcgtcgaac agaaagattt caagacagca gcgcggcggt
      781 aatttggttg tgagcgtctg cgtgttaagt caaaggcgaa gtttgtgtat cttgcgcaag
      841 tgaaatttac ttttcgccgg gcggcgatgc tggccgtgct gctctgcctg ttgctcgtcg
      901 cgacgggcgc gtcggcgcag cgaaaggggc gcggcggcag tagcaatagt cgcggcgaac
      961 agttggagcg cgcggcgtcg ctcattgcga gcgagcagtt ggcggaggcg gaacagcagt
     1021 tgagtcaaat cctgaaagtc gcgcccacgg aagcagtcgc gctcaacctg cttggtgcag
     1081 tccgcgcgaa acagggaaag ctcgacgagg ccgagacgct cttcacacgc gccgtgcgca
     1141 gcgacactga actcgcgggc gcgcacatga atctggctta tctctacctg ctcaagcgcg
     1201 agccggaaaa gtcggcggcg gcgttgaagg aagtgttgcg cattgagcct gcaaacgcgg
     1261 atgcggctta caggctcgcg tggctgctgc actcgcaggg tcgttatgaa gagtgcatcg
     1321 ccgtcgcgga gaagttgaag ggaagcgagg cgcgctccgc tcgtttgctc ggcgtgctcg
     1381 gcgatgccta cttgagaaag ggcgataagg cgggggctgt gtcggcgttg aaaagggcgg
     1441 tcgaactcag tccgggcgag ccgtcgtatc acttcgcgct ggcctccgcg tggctcaggc
     1501 atccgcccga cttgcaggaa gccgaagact ctttccgcaa gttcaacacg ctcagccccg
     1561 gcgacgcgca gggacaactg cacctcggct acgtgctgct caaacagaag aagcacgcgg
     1621 aagcgcgcga gtggctggag cgaagcgcgg gcacgggcgc gggcacgccc gaagccttct
     1681 attacctcgg cctcatcgca caggcgcaga acgaagatgc gcgtgctatc gaactcttcg
     1741 agaagtccat cgcgctcgcg cccgccttcg gacatgttca cgtcgcgctc ggttcggcct
     1801 acctgaaatt gaaggactac gtgcgcgcgc aaactgcgct tgagacgggc gcgaagctga
     1861 gtcccgaaga ttcgaaggcg cactacaatc tcgcgctgct ctacgcgcgg ctgaaacaac
     1921 cggagcgcgc gcgcgaagag atgctcatcg tcgagcgtct caagagtgaa ggcaaagcgc
     1981 gcgacgacgg cgagaacgaa acatacgcgc ccccgccgcc acgctgaccc ggagccggaa
     2041 cgcgctcaag gcaagccggg ttcaacgtca accagtttat gaaagataag atcgtcatct
     2101 catctttagt tttcatgctc gtgcttaact tgcctttcta ttccggcgcg cggcaggcgg
     2161 ggacggacgc gcgtgctgcc gtcactttcg cggaagtcgc gcccaaagcg agcgggatca
     2221 gttgggcgca caacaacgcg cacacgccgg agcgttttct gccggagacg gtcggggcgg
     2281 gcggcgcgtt tttcgattac gacaacgacg gctggttgga catctatctc gtcaacagtg
     2341 gcgcgtcgga cttctacacg cccgcgcgcc cgcttaagaa cgcgctctat cgcaacaacc
     2401 gcgacggcac ttttacagac gtgacggaca aggccggagt ggcgggcgga aaattcggga
     2461 tgggcgcagc cgccgcagac ttcgacggcg acggctggca agacctctac gtcaccaact
     2521 acggcgcgga catcctctat cgcaatcgtg gcgacggcac gtttgcagac gtgacgcaga
     2581 aggcgaatct gacggttgat ggttggtcaa cgtgtgccgt ctggttcgat tacaacgcgg
     2641 acgggcggct cgatctcttc gtctccagct tc
//
LOCUS       DASWNS010000642         5062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_128845, whole genome shotgun sequence.
ACCESSION   DASWNS010000642 DASWNS010000000
VERSION     DASWNS010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128845"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VGB76_22260"
     CDS             complement(<1..458)
                     /locus_tag="VGB76_22260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101665.1"
                     /translation="MLRRITACLNASALVLVSLAPLTIASAKAHAQTTQTETASKIAP
                     ELQQTISVGGSTGSATGIGTGGAAQGPGQIGGSQRVRIVLQTKGRPTDEQRSAIASVE
                     GQISQSYEALNALVLDVPRSSLGSLAARTDVAYISPDRPVASQMSMASETV"
     gene            complement(1283..3529)
                     /locus_tag="VGB76_22265"
     CDS             complement(1283..3529)
                     /locus_tag="VGB76_22265"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY0101666.1"
                     /translation="MRKKANRSSMNNDHHSYSYNNRKTNRVEIERTSSASRQRLILKA
                     VLVLAGIALAAVSAHTLLFTAPQQVQAQVEAPPLALAQNLGSEDLVNPSEIRYRANEK
                     RMRGVMLMMSGKYMIPNVGTATLRQYRGWDPMTTPTPDLTSEAISPGPTLRARLGDTV
                     ELSFLNKIDDRQFAYTFDTNSQPGRSSFGCDQSGTIYPGKDLFPNCFHGSSTANIHFH
                     GTHASPDGLGDNVLVQVMPQANQKDWTPTFNKIFNSGKIPQEWKEMPENYREEQMDLI
                     KKHDDASAAAARKNGLLPPGSLYEANKHMIAAGEWPQYIMGAFPNFFKIPDYSSGEWK
                     AGQAPGTHWYHAHKHGSTSLHILNGLAGAFVIESSREGGYDHVIRKFYGWGSSYGNHE
                     KILVFQQFDPSQNLERTPGNPKGKGSLQVLLNGKFKPTITMRPGDVQLWRLINATVGN
                     EVGVLVGKDLFGSKPGFKFKQTAQDGVQFSRDNYQNQPFLNASAPGGQVPGGFTLAGG
                     NRADLLVQAPTTAGVYEFQSGGNILFFVKVAGTPAALPNGAFPSKAQWAEMPRFLQDL
                     RPPGANDVKNPNSPVKFQWDPGRVKQGRDANNNPPHFMINNKQFGETGEIVDQCMPLN
                     GLQEWVLENWTTIPHPFHIHINPFQVVRIETPTALNKYAPPYKPANNFVWQDVIAIPP
                     AVMSTDGKQITPGRVVIRQTYLDFTGTYVLHCHILAHEDRGMMQLVRVVPASKYPAGC
                     QEAVPDHH"
     gene            complement(3575..4192)
                     /locus_tag="VGB76_22270"
     CDS             complement(3575..4192)
                     /locus_tag="VGB76_22270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101667.1"
                     /translation="MKQINIKRDNTGKVTFDTVSVDTTENVFFTNLDPQAEHWPAFAN
                     QQPFTTNKLGAAPSPNSSQCPVPPPSDLTPPNNQVTYGCQIAGHQNEKGTINVFAPLA
                     AADNTTLASATKGKPIAAQPVVVGGMSPYAISGQQFQVTDKNGNVLKSGAGIGPGLTL
                     NPSPNSDGISVTGTPTMSGTYNFTFTVNDAMGRNLQQTQYVMVVT"
     gene            4509..>5062
                     /locus_tag="VGB76_22275"
     CDS             4509..>5062
                     /locus_tag="VGB76_22275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101668.1"
                     /translation="MARQLKSRPRLLSRLTVAVLVDRAVLVPGSSRFRKGIGDAPLVN
                     TLRRMVEKVSVGAYTDTGGVLKWFAELRPDIVFNLTENVDGDRQKDGYICALLDLLKL
                     PYTGTGPRGMLLCRDKAVSKLIAAREGFKTPEFFVVEGRAPRLPRQLAFPLVVKPRFD
                     DASVGISQASLVRTKEALLERIAYL"
BASE COUNT          973 a         1357 c         1571 g         1161 t
ORIGIN      
        1 accgtctcgc tcgccatact catttgcgag gcgacggggc ggtcggggga gatataggcc
       61 acgtcggtgc gcgccgcgag gctgccgagc gaactgcgcg gcacgtccag aacgagcgcg
      121 ttcagagctt catagctctg gctgatctgc ccttcgacgg aggcaatcgc gcttctctgt
      181 tcgtccgtgg ggcggccttt ggtctggagg acgatgcgaa ctctctggct gccgccgatc
      241 tggcctgggc cttgagccgc gccgccggtg ccgatgcccg tcgcggagcc ggttgaaccg
      301 ccgacgctta ttgtctgctg tagttcgggg gcgatcttcg aggctgtctc ggtttgtgta
      361 gtttgcgcat gagccttggc tgatgcaatg gttaaggggg cgagagaaac cagcacgagg
      421 gcactggcat ttaggcaagc tgtgatacga cgcaacataa taattttccc ttcttctgtc
      481 tggactgggg ttgcgctgtg gggtagcggg ggaacagttc agacatccct tcaatttttt
      541 ttgttttcaa gattaagacc gctgacaggt ctaatgcctg gcgtgtgagg aggcgccggc
      601 atcaggccaa agagtgaagg ccgcgttact ctcggccctt tccctgtcaa cgatctgtca
      661 ttactaatta caatagttgt gccactccgc agaaagtcgc agggaggggg ctaaacacta
      721 ctaataatta gacttaacca cggcaagggg attcaggcga gacacccgga taaatttacg
      781 aattggttac caggtatcca attccgagcg ctgtaaaagc gaattctcat taaaaagcct
      841 taaaacaagg aaataagcgt ctttcctaaa aagtggatag ctattaaaca cttttgtgga
      901 attggatacc tagtatccaa caatcccttg catagaaata ggcaataggg tcttaatacg
      961 agacttgtct gaaagttaca ggacaggagg gagactcggt tcgggctacg ttgcgccgcc
     1021 tgcgggaagc atagcgtcct tacgcacgga cttatggcga gagagattgt ggcgggtctg
     1081 tttgacgtgc gcgcagggct ggacgcgttc gtgaaaatta attatttcgc cgtgcaagag
     1141 caacgccgga cggcacgaac ccgtgccgtc cggcgtaagc gactgctctt ggtgaaataa
     1201 atcctacgct gagctgagca ttctccatcc agtccgcgcg tcaggcgtgg tcggcgcgcg
     1261 cggagagcag cgtcagacgc gcttagtgat gatcggggac ggcctcctgg cagcccgcag
     1321 ggtatttact ggcgggcacg acgcgtacga gttgcatcat gccacggtct tcatgtgcga
     1381 ggatgtggca gtggagaaca tacgtgccgg tgaagtcgag ataggtctgc cggatgacga
     1441 ccctgccggg tgtgatctgt ttgccatccg tgctcatcac cgctggcggg atggcgatca
     1501 cgtcctgcca gacgaaattg ttcgccggtt tatagggcgg cgcgtacttg ttcagcgccg
     1561 tcggcgtttc gatgcgtacc acctgaaacg gattgatgtg gatgtggaaa gggtgcggga
     1621 tggtcgtcca attctcaagc acccactcct gtaaaccgtt gagcggcatg cactgatcga
     1681 cgatctcgcc cgtttcgccg aactgcttgt tattgatcat aaagtggggc gggttgttgt
     1741 ttgcgtcgcg cccctgtttg acgcgtcccg gatcccattg aaatttgacc ggggagttgg
     1801 gattcttcac gtcgtttgcg ccgggagggc gcaggtcttg caaaaatctc ggcatctccg
     1861 cccactgcgc tttggaggga aaagcgccgt tgggcagagc cgccggggtg cctgcgacct
     1921 tgacgaaaaa caggatattc ccgcctgact ggaactcgta gactccagcc gtcgtcggtg
     1981 cctggacgag aaggtctgcg cggtttccgc ccgccagtgt gaatccgccg ggtacttggc
     2041 cgccgggcgc gctggcgttg agaaacggtt gattctggta gttgtcgcgg ctgaactgca
     2101 cgccgtcctg tgcggtctgt ttgaatttga aacccggctt agacccgaac aggtctttgc
     2161 cgacgagcac gccgacttcg tttccgacgg tggcgttgat gagacgccac agttggacat
     2221 cgcccggcct catcgtgatc gtcggcttga acttgccgtt gaggagaact tgcaggctgc
     2281 ctttaccctt ggggttgccg ggcgtccgct caaggttctg agacgggtcg aactgctgga
     2341 agacgagaat cttctcgtgg ttgccgtaag aacttcccca accgtaaaac tttcggatga
     2401 cgtggtcgta accgccttcg cggctcgatt cgatgacgaa cgctccggcc agtccgttga
     2461 ggatatggag cgaggtcgag ccgtgcttat gcgcgtgata ccagtgcgtg cccggcgctt
     2521 gccccgcctt ccactcgccc gaactatagt cgggaatttt gaagaagttg gggaacgcgc
     2581 ccatgatgta ctgcggccac tctccggcgg cgatcatgtg cttgttggcc tcgtagagcg
     2641 aaccgggcgg gagcaggccg ttctttctgg ccgctgcggc ggaggcgtcg tcgtgcttct
     2701 tgatcaagtc catctgctct tcgcgataat tctccggcat ctctttccac tcctgcggaa
     2761 tcttgccgga gttgaaaatt ttattgaacg tcggcgtcca atccttttga ttcgcctgcg
     2821 gcatcacctg cacgagcacg ttatcgccta aaccgtcggg gctggcgtgc gtgccgtgga
     2881 aatgtatgtt ggcggtgctg gaaccgtgaa agcagttggg gaagaggtct ttgccgggat
     2941 agatcgtgcc gctctgatcg cacccgaaac tggagcgacc gggctggctg ttggtatcga
     3001 aggtataggc aaactggcgg tcgtcaatct tgttgaggaa cgaaagttcg accgtgtcgc
     3061 cgaggcgcgc gcgcagcgtc ggacccggcg agatggcctc gctcgtcaga tcgggcgtgg
     3121 gagtcgtcat ggggtcccac ccgcgatact gccgcagtgt cgccgtgccc acgttcggga
     3181 tcatgtattt gccgctcatc atcaacatca cgccgcgcat cctcttctcg ttggcgcggt
     3241 agcgaatctc gctcggattg acaagatctt cgctgccgag attctgcgcc agagccagcg
     3301 gaggagcctc cacctgcgcc tgcacctgct gcggggcggt aaacaacagc gtgtgtgcgc
     3361 tgacggcggc gagggcgatc cccgcgagca ccaagaccgc cttgaggatg agacgctgcc
     3421 gcgaggcgga cgacgtgcgt tcaatttcca cgcggttcgt cttccggttg ttgtagctat
     3481 atgaatggtg gtcgttgttc atgctgctcc tgttcgcttt tttacgcatc aactccttaa
     3541 ctactttgcc cgtctcccta cagacgatgc ctgttcaggt gacgaccatc acgtactgtg
     3601 tctgctggag gtttcgcccc atcgcatcgt tcaccgtgaa cgtgaagttg taggttccag
     3661 acatggtggg cgtaccggtc acagagatgc cgtcgctgtt cggagaaggg ttcaaagtca
     3721 gacccggccc gatccccgcg cccgacttga gcacgttgcc gttcttgtcc gtgacctgaa
     3781 attgctggcc gctgatggca tacggcgaca tcccgccgac caccaccggc tgcgcggcga
     3841 tgggcttgcc tttggtcgca gatgccagcg tcgtattatc cgccgccgcg agcggcgcga
     3901 acacgttgat ggttcccttc tcattctgat gccctgcgat ctggcatccg taggtcacct
     3961 gattgttggg cggggtcagg tcggaaggtg gcgggacggg gcattgactc gaattgggag
     4021 acggagctgc gccgagttta ttcgtggtga atggttgctg attggcaaac gcaggccagt
     4081 gttccgcctg cgggtccaga ttggtgaaaa acacgttctc ggtcgtgtcg acagagacgg
     4141 tatcgaaagt caccttccca gtgttatccc gcttgatatt tatctgtttc atggaacttc
     4201 cctttcgcgt ccgaccgcag ggtaaagggt cttatcgcaa agtgagttta tagaaaggag
     4261 ctgcactata tcacacggtt aagcgcagcc tgcaagaaaa attttcagct acaaatggag
     4321 cagatcgtcg cttcgatagt gagagaaatt tcacccggct caagcgcgaa agaagcattg
     4381 cccggatcac gatagatgat gctataacac acggctcacg ggcgatgggt tgatgacagg
     4441 tcgcggttga cggctcaggc ttcgagcaag atgtccggca aagagtcctt cacggaggcg
     4501 tgctttaaat ggcgagacaa cttaagagtc ggccacgttt gctgagtcgc ctgacagtgg
     4561 cggtactcgt cgaccgcgcg gtgctcgtgc cggggagttc gcggtttcgc aaaggcatag
     4621 gcgatgctcc tttggtgaac acgctgcgtc gcatggtgga gaaagtgtcc gtcggtgcgt
     4681 acacggatac gggcggggtg ttgaagtggt ttgcggagtt gaggccggac atcgtcttta
     4741 atctcacaga gaatgttgac ggcgaccggc agaaggacgg ctacatctgc gcgctgctcg
     4801 acctgctcaa acttccttac acgggcacag gaccacgcgg catgttgctc tgccgcgaca
     4861 aggccgtgag caaattgatc gccgcccgcg aagggtttaa gacgccggag ttcttcgtgg
     4921 tagagggacg cgcgccgcgc ctgccgcgcc aactcgcctt tccgctcgtg gtcaaaccgc
     4981 gtttcgatga tgcttcggtg gggatcagtc aggcttctct cgtgcgtaca aaggaggctc
     5041 ttctggagcg catcgcttac ct
//
LOCUS       DASWNS010000643         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_50939, whole genome shotgun sequence.
ACCESSION   DASWNS010000643 DASWNS010000000
VERSION     DASWNS010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50939"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..342)
                     /locus_tag="VGB76_22280"
     CDS             complement(70..342)
                     /locus_tag="VGB76_22280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101669.1"
                     /translation="MSYLVYFGIVVAAGGVLLLASDAFAHAVSRGVEAMFERVEYRLA
                     LRAREQQVRRRETRVTNATVSATTFRVIEGQRKGSTQQLGVARVAA"
     gene            complement(428..616)
                     /locus_tag="VGB76_22285"
     CDS             complement(428..616)
                     /locus_tag="VGB76_22285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101670.1"
                     /translation="MSELNGTTNTTSARLTQRAARAERNRRLESIKMERRRARRGKWS
                     QEIKPHANLLLQSAKNVN"
     gene            complement(788..1891)
                     /locus_tag="VGB76_22290"
     CDS             complement(788..1891)
                     /locus_tag="VGB76_22290"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0101671.1"
                     /translation="MSAVLEMVAKQDADGRNDSYVSTPEGAATPPPQAVLTQMLAAPL
                     VTQALRVVAELGIADLLAQGARSVDELAAETGAHAPSLYRFMRALASCGVFAETKEER
                     VFALTPMAELLRADVEGSLRSLAIFMGADWHWQVWGDAVYSARTGKVAWEHVHGKEVF
                     PYFAEHAEAARVFDDAMTSLSKMVSKAVVEAYDFSSIGKLADIAGGHGSLLAAILRAN
                     SHLRGLLFDVPQVIAGAQSQLEAEGVSGRCELASGDFFESVPEGADAYLMKHIIHDWE
                     DARAIAILKNCHRAMPETGKLLLVEMVIPPGNEPHFGKIQDLEMMLSPGGLERTAEEF
                     RQLFAAAGFELTRIIPTASPMSVIEGVKTSTQA"
BASE COUNT          526 a          897 c          672 g          575 t
ORIGIN      
        1 ctggagcggg ctgagaaaga acacgccgtc ggctacacat acctgcgcgt cgatccgcgc
       61 ctcgacaatc tacgcggcga cccgcgctac gccaagctgc tgcgttgaac ctttacgctg
      121 cccttcgatc acgcgaaacg tcgtcgcact cacggtcgca ttcgtaaccc gggtttcgcg
      181 ccgtcgcacc tgctgctccc gcgctctcaa ggcgagccga tattcaaccc gctcgaacat
      241 cgcttcgacc ccgcgcgaca ccgcgtgcgc gaaggcgtcc gaggccagca gcaacacgcc
      301 gcccgccgcc accacaattc cgaaatagac tagataactc atcgcttctc actctccctc
      361 gccgtgtcgt tccggcttcg aactctctca aaggttgata gtttttttaa agataactct
      421 ttgcgctcta gttcacgttc ttcgcgctct gcaaaaggag attcgcgtgc ggctttatct
      481 cctgcgacca tttcccgcgc cgcgcacggc gtcgctccat cttgatggac tccagacgcc
      541 ggtttcgctc ggcacgtgca gcgcgttgcg tcagccgcgc tgaggtcgta ttcgttgtgc
      601 cgttgagttc cgacattgct ctgctcttcc tctccaaatc tttctgcctc tattaactaa
      661 cgcgcaacgc gaacccgaaa gggatcatgc ttttacgcct tcgacccgcc ttaaaagtga
      721 aaggctccgg cagttacccg gagcctttca cgcctgctta ctctctgcct gcaacggcat
      781 ctacgtctca agcctgcgtg ctggtcttca ctccctcaat cacgctcatc ggcgacgcgg
      841 tggggatgat tcgcgtcaac tcaaacccgg cagcggcgaa cagttggcga aactcttctg
      901 cggtgcgctc aagcccgccc ggtgagagca tcatttcgag gtcttgaatc tttccgaaat
      961 gcggctcgtt gcccggcggg ataaccattt cgacgagcag cagtttgccc gtctccggca
     1021 tcgcgcgatg gcagttcttg agaatggcga tagcgcgcgc gtcttcccaa tcgtgaatga
     1081 tgtgcttcat caagtaagcg tccgcgccct cgggcacgct ctcgaagaag tcgcccgacg
     1141 cgagttcgca acgccccgat acgccttcgg cttcgagttg actctgcgcg cccgcgatca
     1201 cctgcggcac gtcgaagagc aacccgcgca ggtgcgagtt ggcgcgaaga atggcggcga
     1261 ggaggctgcc gtggccgccc gcgatgtccg ccagtttgcc tatggaggaa aagtcgtaag
     1321 cctctacgac ggccttcgac accatcttcg aaagactcgt catcgcatcg tcaaagacac
     1381 gcgccgcctc cgcgtgctcc gcgaaatacg gaaagacttc cttgccgtgc acatgctccc
     1441 acgccacctt gcccgtccgc gcgctgtaga ccgcgtcgcc ccacacctgc cagtgccagt
     1501 ccgcgcccat gaagatcgca agcgagcgca gcgagccttc aacgtccgcg cgcaaaagtt
     1561 cggccatcgg cgtcaacgcg aagacgcgtt cctctttcgt ctcggcgaag acgccgcaac
     1621 tcgcgagcgc gcgcatgaag cgatagagcg acggggcgtg cgcgcctgtc tcggcggcaa
     1681 gttcgtcaac gctgcgcgcg ccttgcgcca gcaggtcggc gatgccgagt tctgccacca
     1741 cgcgcaacgc ctgcgtgacg agcggcgcgg cgagcatctg cgtcaacacg gcctgcggcg
     1801 gcggcgttgc cgcgccctcc ggcgtcgaaa cataactatc gttccgcccg tcggcgtctt
     1861 gtttggcgac catttccaac actgcactca tttttttcaa ctcctctcga cttctcatcg
     1921 aacattcaaa attcgtttcc aattattaac gcgcgctccg cccacccgaa ggatcacacg
     1981 ccgcgatctt ttttcacacg ccgcgttcgg aaaaatctct ctgccccttt tccgcccacc
     2041 atgcccggct cttccgcgcg ctctctctgc ctgcgatggt gaaagctttt tgagacaccc
     2101 acgaccgcag acacacaggc cgcacagata agagattaaa taaagagact gacgcccgta
     2161 gtaatttctt gacagacaac gcaaataagg taaaattttt ttcgcaatca attccccctt
     2221 caaggcatca taattcggtt ctctattgcg cactcgcagc acccctcctc aagtgctttc
     2281 ttgtttgcca tgaactttcg caggcggctg cggaatctcg ttcttacgcg cgaggctccc
     2341 tgtcgtcccg cttaagttta aggccgtcag ttagacgaag ggacgacgac atccggctgc
     2401 gtcgcgccgc tacagggttt tccaacgccc gcgcgcccgc acaccaacag gcaacgtcca
     2461 ctcgtattca tttcaatctt aattaacttc cggctccact cctcagccgt agatcaccac
     2521 aggacggaga actaaaccac tatgcactga aagtgcatag atttccgcag gactgaaagt
     2581 ccgcctcatt ccaagatgaa tgcgtcgctg tcctgattag attgttgccg atgatgctca
     2641 agatattcct gcaccaccgt ttcgctgacg
//
LOCUS       DASWNS010000644        11049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_206904, whole genome shotgun sequence.
ACCESSION   DASWNS010000644 DASWNS010000000
VERSION     DASWNS010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206904"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VGB76_22295"
     CDS             complement(<1..481)
                     /locus_tag="VGB76_22295"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY0101672.1"
                     /translation="MRLDPNLSEWLNLFARWFHVFAGILWVGTTYYFTWLDARLSEAE
                     ATAPGGEKPQVWMVHSGGFYVVEKQKQPGTETLHWFRWEAALTWLSGMVLLVLVYYLG
                     GALIDTDVYDISLPAAVAFSIGLLVVGWVVYDLLMLSPLGRNEIAFACVAYALLVGVA
                     "
     gene            complement(487..828)
                     /gene="uraH"
                     /locus_tag="VGB76_22300"
     CDS             complement(487..828)
                     /gene="uraH"
                     /locus_tag="VGB76_22300"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEY0101673.1"
                     /translation="MSAITTHILDLSSGQPAQGVAVVLEVQAGEGWRVLGRGKTDADG
                     RLKSLLPDDLNPEAGVYRLTFRTSDYFTARGIESFYPEVSISFTVGDGAAHYHVPLLV
                     SPFGYSTYRGS"
     gene            complement(922..1440)
                     /gene="uraD"
                     /locus_tag="VGB76_22305"
     CDS             complement(922..1440)
                     /gene="uraD"
                     /locus_tag="VGB76_22305"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF010372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEY0101674.1"
                     /translation="MTMHEGLARLNALDAAEAERELLKCCGSNAWAREMAAQRPFGET
                     RELLTAADDIWRSLDAQDWLEAFAAHPKIGGRKAARAQDAQAQNWSEQEQSGARHAEQ
                     STLDELAAANRAYEEKFGHIFIVCATGKTAGEMLALLRLRLPNDAADELRNAAEEQRK
                     ITRLRLEKLLTT"
     gene            complement(1478..2482)
                     /gene="alc"
                     /locus_tag="VGB76_22310"
     CDS             complement(1478..2482)
                     /gene="alc"
                     /locus_tag="VGB76_22310"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEY0101675.1"
                     /translation="MTDFTELVDLAAERVGGAVLYANDDFFAPKENLLKASAPVFIEG
                     KYTDRGKWMDGWESRRRRTPGFDWCLIRLGLPGIVRGVVVDTSYFRGNYPEQCSLEAC
                     AMDGQPDVEQLLDEGVEWSELLPVSGLRGDAENSFQVQTERRATHLRFRIYPDGGVAR
                     LRVYGEVVPDWTHIKAGGGRVDLAAVESGALVLASSDMFFGHRHNLIMPGRAANMSDG
                     WETKRRRGAGHDWVVIKLGARGRLHQVEVDTSHFKGNYPESCSLEGCDAGGAVGNAGA
                     LDGYAWTEILPRTKLQAHTRHHFTNELKPHTAITHARFNIYPDGGVSRLRLYGNLEDV
                     "
     gene            complement(2479..3906)
                     /gene="allB"
                     /locus_tag="VGB76_22315"
     CDS             complement(2479..3906)
                     /gene="allB"
                     /locus_tag="VGB76_22315"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEY0101676.1"
                     /translation="MDSPDTQLSAAEFVIRARRVLLPGEIAAAAIHVRDGRIHAINAF
                     EDAPRGLPLVEVEAEAVVLPGLVDTHVHINEPGRTEWEGFRTATRAAAAGGVTTLVEM
                     PLNSIPATTTRESLETKRAAAGGQCYVDVGFWGGLVPGNAPELGRMFECGVFGFKCFL
                     VPSGVDEFEHVTEPDLRAAMPELARLGALLIVHAETPAPIEAALAGLTHEDFARDAKS
                     ADPRRYETFLRSRPKAAEDEAVALMVRLSRETGARVHIVHHSSSASLPSLRAAKAEGL
                     KFTAETCPHYLAFAAEEVADGATEFKCCPPVRERENRERLWDALDEGVLDMIVSDHSP
                     CPPELKRREEGDFLAAWGGIASLQLRLPVVWTEARARGYGIERVVEWTAAAPARLVEL
                     EGRKGALTVGCDADIVVFRPDETFRVEPALLQHRHKLTPYAGQTLSGVVETTYLRGVK
                     IYERGEFMNETATGVLLARQDEARR"
     gene            complement(3899..4771)
                     /locus_tag="VGB76_22320"
     CDS             complement(3899..4771)
                     /locus_tag="VGB76_22320"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY0101677.1"
                     /translation="MRAYLPDYELRAPQTLSDTLDVMAREPGVWRAFAGGTDLMVLLE
                     FGLLKHRNFLSIRHLKELGGIEVTPSEVRLGALTTYAEIQRHETMRREFSLLCEAARE
                     TGGIAIQNRGTLGGNIVNASPAADSPPALLVYDAVVEVVSTRGTRSLPYQGFHTGYKQ
                     MDLREDELLYRIRLPRATRDLKTRYRKVGTRKAQAISKICFAAGARFGSGGRISDLRV
                     ALGSVAPTVVRCTRTEDLLRDQTLDEATIKAACAMLAQEITPIDDMRSTAQYRLRVAA
                     NLLANFLSTLKADG"
     gene            complement(4768..5259)
                     /locus_tag="VGB76_22325"
     CDS             complement(4768..5259)
                     /locus_tag="VGB76_22325"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY0101678.1"
                     /translation="MTDSARGVDVNCTVNGVRQTLRVLPMARLLDVLREDLRLTGTKE
                     GCGEGECGACSVLVDGELVNSCLVPVLQVEGARIQTIEGIADGAQLHAVQEAFIECGG
                     AQCGICTPGMIMAAVSLLAGNPQPTDADIRSGLAGNLCRCTGYMKIFDAVVRACQRAG
                     VEA"
     gene            complement(5293..7536)
                     /locus_tag="VGB76_22330"
     CDS             complement(5293..7536)
                     /locus_tag="VGB76_22330"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY0101679.1"
                     /translation="MPTTESDGRPVPRRKEGRSKVTGAARYVDDLHPPGMLYGATVRS
                     PAPRGRIRSITFGAGIPWDEFVVVTAKEIPGENAVALILLDQPYLATEFVNHTEEPIL
                     LLAHADKHLLERARRAVSVEIEELPPVFTLEESLAKKEIIWGEDNVFKSYLMDKGNVD
                     EVWETADLVVEGEYTTGAQEQLYIETNGVIAVANEAEGVTVWGSMQCPYYVHKALLKL
                     FALPPERVRVVQCETGGGFGGKEEYPSLIGGHAALLSWKAGGRPVKIVYDRAEDMAAT
                     TKRHPSRTRHRTAVTREGKLLAMEIEFVVDGGAYATLSAVVLSRGTIHAAGPYSCPNV
                     RIRSRAVATNVPPHGAFRGFGAPQSIFALERHFDKVAHALGLSPEEFRRRNFIREGET
                     TATGQVIREPVDMAGLLDRAFELTDYQSKRERFTRENAAAGATVRRGIGFASFMHGAG
                     FTGSGEDYLKSIVGAEAAGDGRVVILTASTEIGQGTNTIFAQIASDALKLDYDLIEMA
                     QPDTGNVPDSGPTVASRTCMVVGKLVESAVLGLRQTLQGAGLLAAGYTQDEWCEACRL
                     YVERYGALKSFSQYKPPPGVHWDDEKYQGDAYGAYAWAVYVAEVTVDLLTYEVRVEDF
                     VAVQEVGKVVHPVLAAGQIEGGVAQAIGFTLYENVVWQNGRMVNNGMTNYVMPTSADL
                     PPIRVHFEEQPYAFGPAGGAKGIGELPMDGGAPAILNAIEQATGVCFTHVPLMPEALM
                     EELSEAR"
     gene            complement(7724..9919)
                     /locus_tag="VGB76_22335"
     CDS             complement(7724..9919)
                     /locus_tag="VGB76_22335"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0101680.1"
                     /translation="MSFAHTRRALSPLRLLIAVILLTQILPAAFAQRSEAGGATGETP
                     KSPIYPAAKKVEQLDDYHGTKVADPYRWLEDLDTAETKAWVEAQNRLTNAYLAEIPAR
                     KRIKERLTKLWNFERFGIPFREGNRYFYMKNDGLQNQYVLYTAGSLTDEPRVLLDPNK
                     LSADGTVALAGTAISRDGKLMAYGLSASGSDWSEWKVRNIETGQDLPDTIKWVKFSGA
                     SWTPDGKGFFYSRYDEPKAGSQLAGVNEFQKLYYHRLGTPQAEDTLIYERRDQKDWGF
                     SGDVTEDGRYLIISVWVGTDVKNRVYYKDLQTKDAPVVKLLDDFDAAYNFIDNDGAVF
                     WFMTDKDAPRNKIVAIDTRQPERANWKVVIPEAAETIEGVNVVGNMFIVSYLKDARSQ
                     VKVHHLDGSFVREVSLPGLGSASGFGGKRTDKETFYAYGSFTAPYPIYRYDVATGKST
                     LFRQPKVDFNPNDYETKQVFFNSKDGTRVPMFITHKKGLKLDGTNPTLLYGYGGFNVS
                     ETPGFSVSRMVWMEMGGVFALANLRGGGEYGKDWHEAGMKLKKQNVFDDFIAAAEWLI
                     SNKYTSTPKLAIQGGSNGGLLVGAAITQRPELFGAALPAVGVMDMLRFTKFTIGAAWA
                     SDYGSPDNPEEFKALYAYSPLHNLKPGRSYPPTLITTADHDDRVWPGHSFKFAAAMQA
                     AQGGSAPVLIRIETKAGHGAGKPTSKIIEEYADLWGFLVRTLNMNVPAS"
     gene            complement(10152..>11049)
                     /locus_tag="VGB76_22340"
     CDS             complement(10152..>11049)
                     /locus_tag="VGB76_22340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101681.1"
                     /translation="TSYNTTPKPKARLINWHVHNFSFISHDFQGRSQAWESETNFQFF
                     FQRQFNIGAAYEYAYERLIEEEFGARRSATLAGAFAGESSERSSRKNHYFFFGGYRPS
                     KKYSVNWRALYRDGHFDYDFGAGRRFPRVSPVAIAAREAAARGLCDEEDEPLPAVCHA
                     PLDPGRGGLFELRATLAYQPTNALRSTLDYTKSHLRRYDTDLVAFDVNLLSWRTTYQF
                     TRFLFARARIDYETLPRRARGQFLLGWTPNPGTAFYVGYNDDANINTFSPINGQLEPG
                     FRRNGRTFFIKMAYLFRRSFGG"
BASE COUNT         1939 a         3579 c         3391 g         2140 t
ORIGIN      
        1 atgcgacgcc gacgagcagc gcgtaggcca cgcacgcgaa cgcgatctcg tttctgccga
       61 gcggcgagag catcaacaga tcatagacga cccagccgac aaccagcagc ccgatgctga
      121 acgcgacggc ggcgggaagg ctgatatcgt aaacgtccgt gtcgatgagc gcgccgccga
      181 gatagtaaac gagcactagc aagaccatgc cgctcagcca cgtcagggcg gcctcccaac
      241 gaaaccagtg gagcgtctca gtgcccggct gcttctgctt ttcgaccacg tagaaaccgc
      301 cgctgtgcac catccagacc tgcggcttct cgcctcccgg cgccgtcgct tccgcttccg
      361 agagccgcgc gtcgagccag gtaaaatagt aggtcgtgcc gacccagagg atgcccgcga
      421 agacgtgaaa ccagcgcgcg aagaggttga gccattcgct cagattgggg tcgagtcgca
      481 ttcaatttaa ctcccgcgat aggtggaata gccgaacggg ctgacgagca gcggcacatg
      541 ataatgcgcc gccccgtcgc cgaccgtaaa actgatcgag acttccgggt agaaactctc
      601 gatgccgcgc gccgtgaaat agtccgacgt gcgaaacgtc agccggtaca cgcccgcctc
      661 cgggttgaga tcatccggca gcaaactttt aagccgcccg tcggcgtccg tctttccccg
      721 ccctaacact cgccagccct ccccggcctg cacttcgagg acgaccgcca cgccctgcgc
      781 cggttgcccg ctcgaaaggt cgagaatgtg tgtcgtgatc gcgctcataa ctaaaaagtc
      841 cccggtcgca agttccaagt ctcaattcgc aagtccccgg tcgcaggctg gcgaggcttt
      901 cagacccgcg acttttaccg ttcatgttgt caacaacttt tccaaacgta gtcttgtgat
      961 cttgcgttgt tcttcggcgg cgttgcggag ttcgtcggcg gcgtcgttcg gcaggcgcaa
     1021 gcgtagcagc gcgagcatct ctccggcggt tttgcccgtc gcgcagacga tgaagatgtg
     1081 gccgaacttc tcttcgtagg cgcggttggc ggcggcaagt tcgtcgagcg ttgactgctc
     1141 cgcgtggcgt gctcccgact gttcctgctc cgaccagttc tgcgcctgcg cgtcctgcgc
     1201 gcgcgccgcc ttgcgcccgc cgatcttcgg gtgcgcggca aacgcttcga gccagtcctg
     1261 cgcgtcgaga cttcgccaaa tgtcatccgc cgccgtcaac aactcacgcg tctcgccgaa
     1321 cggcctctgc gctgccatct cgcgcgccca cgcgtttgag ccgcagcact tgaggagttc
     1381 cctctccgcc tccgccgcgt cgagcgcgtt caggcgtgcc agaccttcgt gcatagtcat
     1441 aattcaaacg tgctgcgcgt gtcgctcgtg agcggtgcta cacatcttcc agattcccgt
     1501 agagccgcag gcgactgacc ccgccgtccg ggtagatgtt gaagcgtgcg tgcgtgatcg
     1561 ccgtgtgcgg cttcaactcg ttcgtgaagt ggtggcgcgt gtgcgcctgt aatttcgtgc
     1621 gcggcagaat ctccgtccag gcgtaaccgt cgagcgctcc ggcgttgccg actgcgcccc
     1681 ctgcgtcgca accttcgagc gagcagcttt cagggtagtt gcccttgaag tgcgacgtgt
     1741 caacttcaac ctgatgcagc cgcccgcgcg ctccgagttt gatgacgacc caatcgtggc
     1801 ctgcaccgcg tcgtcgtttc gtctcccagc cgtcgctcat gttcgcggcg cgacccggca
     1861 tgatcaaatt atgtctatga ccgaagaaca tgtcgctcga cgcgagcacc agcgcgcccg
     1921 actcgaccgc tgccagatcg acgcgcccgc cgcccgcttt gatgtgcgtc caatccggca
     1981 cgacctcgcc atacacgcgc agccgcgcca cgccgccgtc cggataaatc ctgaaccgca
     2041 gatgcgtcgc acgccgttcg gtttgaactt gaaacgaatt ctccgcatcg ccgcgcaggc
     2101 cggacacggg caacaactcg ctccactcga ccccttcatc caacagttgc tcgacatcgg
     2161 gttgcccgtc catcgcacag gcttcgagcg aacactgctc cggatagttt cccctgaaat
     2221 aactcgtgtc cacgacgacg ccgcgaacga tgcccggcag accgagtcga atgaggcacc
     2281 agtcgaaccc cggcgtacgg cgacggcgcg actcccaacc gtccatccac ttgccgcgat
     2341 ccgtgtactt gccttcgatg aagacgggcg cgctcgcctt caggagattc tccttcggcg
     2401 cgaagaaatc gtcgttggca tagagcaccg cgccgcccac gcgctccgcc gccagatcaa
     2461 ccagttccgt gaaatccgtc atcgtcgtgc ctcatcctgc cgcgcgagca gcacgcccgt
     2521 cgccgtttcg ttcataaatt ctccgcgttc ataaatcttc acgccgcgca ggtacgtcgt
     2581 ctcaaccacg ccgctcaaag tctgccccgc ataaggcgtc agcttgtgcc ggtgttgcag
     2641 gagcgcaggc tcgacgcgaa aggtttcgtc cggacgaaag acgacgatgt ccgcatcgca
     2701 cccgaccgtg agcgcgccct tccgcccctc cagttcgacc agacgcgcgg gcgcggcggc
     2761 tgtccattcg acgacgcgct cgattccgta accgcgcgca cgcgcctctg tccacacgac
     2821 gggcaggcgc aattgcagag aggcgatgcc gccccacgcc gcgaggaagt cgccctcttc
     2881 gcgccgcttc aactccggcg ggcacggcga atggtccgag acgatcatgt cgagcacgcc
     2941 ttcatcgagc gcatcccaaa gcctctctcg gttctcgcgt tctcgaaccg gcgggcagca
     3001 cttgaactcg gtcgcaccgt ccgccacctc ttccgccgcg aacgccaggt agtgcgggca
     3061 cgtctcggcg gtaaacttca acccctccgc tttcgccgcg cgcagagagg gcagcgacgc
     3121 agacgacgaa tgatgcacga tatgaacgcg cgcgcccgtc tcgcgcgaca gccggaccat
     3181 caacgccacg gcttcatcct ctgccgcctt cgggcgcgag cgtagaaacg tctcgtagcg
     3241 tcgcgggtcg gcgctcttcg cgtcgcgcgc aaagtcctcg tgcgtcagcc ctgcgagcgc
     3301 ggcttcaatc ggcgcgggcg tctccgcgtg gacgatcaag agcgcgccga ggcgtgctaa
     3361 ctccggcatc gccgcgcgca agtccggctc cgtgacgtgc tcgaactcgt ccacgcccga
     3421 cggcacgaga aagcacttga agccgaacac gccgcactcg aacatgcgcc cgagttccgg
     3481 cgcgttgccc ggcacaagcc cgccccagaa gcctacgtca acgtaacact gccccccggc
     3541 tgccgcacgc ttcgtctcca gactttcgcg cgtcgtcgtc gccggaatac tgttcaaagg
     3601 catctctacg agcgtcgtca cgccgcccgc cgccgccgcc cgcgtcgccg tccgaaagcc
     3661 ctcccattcg gtgcgtcccg gttcgttgat gtgcacgtgc gtatcaacca accccggcaa
     3721 gacgaccgct tcggcctcaa cttcgaccag cggcaacccg cgcggcgcat cttcaaaggc
     3781 attgatcgca tggatgcgcc cgtcgcgcac gtgaatggca gccgcagcaa tttcgccggg
     3841 cagaaggacg cggcgcgcgc gtatgacaaa ctcagccgcg ctcaactgtg tgtcgggact
     3901 atccatcggc cttgagagtt gaaaggaaat tcgccagcag gttggcggcg acgcgcaggc
     3961 gatactgcgc cgtcgagcgc atgtcgtcaa tcggcgtgat ctcttgggcg agcatcgcgc
     4021 acgcggcctt gatcgtcgcc tcgtcgagcg tctgatcacg caacaggtct tccgtacgcg
     4081 tgcagcgaac gacggtcggc gcgacgctgc cgagcgcgac gcgaagatcg cttatgcgcc
     4141 cgccgctgcc gaagcgtgcg cccgcggcga agcagatttt cgagatggcc tgcgctttgc
     4201 gcgtgccgac tttgcgatag cgggtcttga gatcgcgcgt cgctcgcggc aggcggatgc
     4261 ggtagagcag ttcgtcctcg cgcaagtcca tctgcttgta gccggtgtgg aatccttgat
     4321 agggcagact gcgcgtgccg cgcgtcgaga caacttcgac gacggcgtca tagacgagaa
     4381 gcgcgggcgg cgagtcggcg gcgggcgaag cgttgacgat gttgccgccg agcgtgccgc
     4441 gattctggat agcgatgccg cccgtctcgc gcgccgcctc gcacagaagc gagaactcgc
     4501 gccgcatcgt ctcgtggcgt tgaatctccg cgtaggtcgt cagcgcgccg agcctgactt
     4561 cggagggcgt gacctcgatg cctccgagtt ccttgagatg ccggatgctc aaaaaattac
     4621 ggtgcttcaa gaggccgaac tcaagcagca ccattaggtc ggtgccgccc gcgaacgcgc
     4681 gccacacgcc cggctcgcgc gccatcacgt ccaacgtatc gctcaaagtc tgcggcgcgc
     4741 gaagttcgta gtcgggcagg taagctctca cgcttcaact cccgcgcgct ggcaggcgcg
     4801 aaccaccgcg tcgaagattt tcatgtagcc cgtgcagcgg cagagatttc cggcgaggcc
     4861 gctgcggatg tctgcgtcgg tgggctgcgg gttgcccgcg agcagactta ccgcggccat
     4921 gatcatgccc ggcgtgcaga tgccgcactg cgcgccgccg cactcgatga acgcctcctg
     4981 cacggcatga agctgcgcgc catcggcgat cccctcgata gtctgaatcc gcgcgccttc
     5041 aacctgaaga accgggacga gacaactgtt gacgagttcg ccgtcaacga gcacggagca
     5101 tgcgccgcac tcgccctcgc cgcagccctc cttcgtgccg gtcaggcgca agtcttcgcg
     5161 cagcacgtcg agcaggcgcg ccatcggcag cacgcgcaaa gtctgccgca cgccgttcac
     5221 cgtgcagttc acgtcaactc cgcgcgcgct gtcagtcatg cttcacttcc ggctctgaat
     5281 ttttcggcct gctcatctcg cttcgctcaa ctcctccatc aaggcttcgg gcatgagcgg
     5341 gacgtgtgtg aagcaaacgc ccgtagcctg ctcgatggcg ttcaggatgg cgggcgcgcc
     5401 gccgtccatc gggagttcgc cgatgccctt cgcgccgccc gccggaccaa acgcataagg
     5461 ctgctcctcg aagtggacgc gaatgggcgg gaggtctgcg gaggtcggca tcacgtagtt
     5521 agtcatcccg ttgttgacca tccgaccgtt ttgccagacc acattttcgt agagcgtgaa
     5581 gccgatggcc tgcgccaccc cgccctcgat ctgtcccgcc gcgagaaccg ggtgcacgac
     5641 cttgcccacc tcctgcaccg ccacgaaatc ctcgacgcgc acttcatacg tcaacagatc
     5701 aaccgtcacc tccgcgacat agaccgccca cgcgtaagcc ccgtaagcgt cgccctgata
     5761 cttctcgtcg tcccaatgca cgccgggcgg cggcttgtac tggctgaacg acttcaaagc
     5821 cccgtaacgc tcgacgtaca ggcggcacgc ctcgcaccat tcgtcctgcg tgtagcctgc
     5881 ggcgagcaag cccgcgccct gcaaagtctg tcgcaaaccg agcacggcgg attcgacgag
     5941 tttgcccacg accatgcagg tgcgcgaggc gaccgtcgga ccactatcgg ggacgttgcc
     6001 cgtgtccggc tgcgccatct cgatcagatc gtagtcgagt ttcaacgcat ccgacgcgat
     6061 ctgcgcgaag atggtgttgg tgccctgccc gatctccgtg ctcgccgtga ggatcacgac
     6121 gcgcccgtcg cccgcagctt ctgcgccgac gattgatttc agatagtctt cacccgaacc
     6181 cgtgaagccc gcgccgtgca tgaaggaggc gaagccgatg ccgcgtcgca cggtcgcgcc
     6241 tgcggcggcg ttctcgcgcg tgaagcgttc gcgcttggat tgatagtcgg ttaattcaaa
     6301 ggcgcggtcc agcaagcccg ccatgtcaac cggctcgcgg atcacctgcc cggtcgccgt
     6361 cgtctcgccc tcgcgtatga agttgcggcg acgaaactct tccggactca agccgagcgc
     6421 gtgcgccact ttgtcgaagt ggcgctccag agcaaagatc gactgcggcg cgccgaagcc
     6481 ccggaacgcg ccgtgcggcg ggacgttcgt ggcgacggcg cggctccgga tgcgcacgtt
     6541 cgggcaactg tatggtccgg ctgcgtggat cgtgccgcgt gagaggacga ccgccgagag
     6601 cgtcgcgtat gcgccgccgt caaccacgaa ctcgatctcc atggcgagca gttttccttc
     6661 gcgcgtgacg gcggtgcgat ggcgcgtcct tgaagggtgc cgcttcgtgg tcgcggccat
     6721 gtcttcggcg cggtcgtaaa caatcttgac cgggcgcccg cccgccttcc aagagagtag
     6781 tgcggcatgt cctccgatca acgaaggata ctcttccttg ccgccgaagc cgccgcccgt
     6841 ctcgcattgc acgacgcgca cgcgttcggg cggcagcgca aacaatttca agagcgcctt
     6901 gtgcacgtag tacgggcact gcatcgaacc ccagaccgtg acgccctccg cttcgttcgc
     6961 cacggcgatc acgccgttcg tctcgatgta caactgctcc tgcgcgcccg tcgtatattc
     7021 gccttcgacc acgaggtcgg cagtctccca aacctcgtcc acgtttccct tgtccatgag
     7081 atacgacttg aagacgttgt cttcgcccca gatgatctct tttttggcga gcgactcttc
     7141 gagcgtgaag acgggcggca gttcttcgat ctccacgctg acggcgcggc gcgcgcgttc
     7201 gagcaggtgc ttgtcggcgt gtgcgagcag caggatcggt tcttccgtgt ggttgacgaa
     7261 ctccgtggcg agatacggtt ggtcgagcag gatcagggcg acggcgttct cgccggggat
     7321 ctctttcgca gtgacgacga cgaactcgtc ccaagggatg cctgcgccga aagtgatgga
     7381 gcgaatgcga ccgcgcggcg cggggctgcg cacggtcgcc ccgtagagca tgccgggcgg
     7441 gtgcaggtcg tcaacgtagc gcgccgcgcc cgtcaccttg ctgcgtccct ctttacggcg
     7501 cgggacgggt cgcccgtcgc tctctgttgt cggcatgtct cgtgtttctt gcggcatcac
     7561 gtcagaaaag ttgggctatt ctgcttgcgc cgctcatcta tgtcaatcgc cgtgctctcc
     7621 cgaccgagtt gccggtgctc ttcacccgca caaatctaga gacagccgga aggcatttcg
     7681 ccttcgcggc tgtctctcgt atgcgtgcgc cgaacccgta gctttaggag gcaggaacgt
     7741 tcatgttcag agtgcgcacg aggaagcccc agaggtctgc gtactcttcg atgatcttcg
     7801 aagtgggttt gcccgcgccg tgtccggctt tcgtctcgat gcgaatgagg acgggcgcgc
     7861 tgccgccctg cgccgcctgc atcgccgccg caaacttgaa gctatgaccc ggccacacgc
     7921 ggtcgtcgtg atcggcggtg gtgattaagg tcggcgggta gctgcgcccc ggtttcaggt
     7981 tgtgcagcgg cgagtaggcg tagagagctt taaactcttc cgggttgtcg ggcgagccgt
     8041 agtcggaagc ccacgccgcg ccgatggtga acttcgtgaa gcggagcatg tccatcacgc
     8101 cgacagcggg gagcgccgcg ccgaatagtt cgggtcgctg cgtgatggcc gcgccgacga
     8161 gcaggccgcc gtttgagccg ccctgaatgg cgagtttcgg cgtcgaagtg tacttgttgc
     8221 tgatgagcca ctcggcggcg gcgatgaaat cgtcaaagac gttctgcttc ttcaacttca
     8281 tcccggcctc gtgccagtcc ttgccatact cgccgccgcc gcgcaaattt gccagcgcga
     8341 agacgccgcc catctccatc cagaccatgc ggctcacgga gaagccgggc gtctccgaga
     8401 cgttgaaacc gccgtagccg tagagcagcg tcgggttcgt gccgtcgagt ttcagaccct
     8461 tcttgtgcgt gatgaacatg gggacgcgcg tgccgtcctt gctattgaag aagacctgtt
     8521 tcgtttcgta gtcgttcggg ttgaagtcaa ccttcggctg gcggaagagc gtgctcttgc
     8581 ccgtcgccac gtcgtagcgg tagatgggat agggcgcggt gaaactgccg taggcgtaga
     8641 acgtctcttt gtccgtgcgc ttgccgccga agccggacgc ggagccgagg ccgggcaggc
     8701 tcacttcacg cacgaaactg ccgtcgaggt gatgcacctt cacctgcgag cgcgcgtctt
     8761 tgagatagga gacgatgaac atgttgccca cgacgttcac gccctcaatc gtctccgccg
     8821 cttcggggat gaccactttc cagttcgcgc gttcgggctg gcgcgtgtcg atggcgacga
     8881 tcttgttgcg cggcgcgtct ttgtcggtca tgaaccagaa gaccgcgccg tcgttgtcta
     8941 tgaagttgta agctgcgtcg aaatcgtcga gcagtttgac gacgggcgcg tccttcgttt
     9001 gcaggtcttt gtaatagacg cggttcttca cgtccgttcc cacccagacg gagatgatca
     9061 ggtagcgtcc atcttcggta acgtcgccgc tgaagcccca gtccttttga tctctgcgtt
     9121 cgtagatgag cgtgtcctcg gcctgcggcg tgccgagacg gtggtagtag agtttctgaa
     9181 actcgttgac gcccgcaagc tggctcccgg ccttcggctc gtcgtagcga ctgtagaaga
     9241 agcccttgcc gtcgggcgtc caagacgcgc cggagaactt cacccacttg atcgtatcgg
     9301 ggaggtcttg cccggtttcg atgtttctta ctttccactc cgaccagtcg gagccggaag
     9361 ccgacagccc gtaggccatc aactttccgt cgcggctgat ggccgtgccc gccagcgcga
     9421 ccgtgccgtc ggcagagagt ttgttcgggt cgagcaggac gcgcggttcg tccgtgagcg
     9481 agccagcggt gtagagcacg tattgattct ggaggccgtc gttcttcatg tagaaatagc
     9541 ggttgccttc gcggaagggg atgccgaagc gttcgaagtt ccagagtttc gtcagacgct
     9601 ccttgatccg cttgcgcgcg ggaatctccg caagataagc gttcgtcaga cggttctgcg
     9661 cttcgaccca agccttcgtc tcagccgtgt ccaaatcttc gagccagcga taggggtcgg
     9721 cgaccttcgt gccgtgataa tcgtccaatt gttcaacttt cttcgccgcc ggatagatgg
     9781 gcgacttcgg tgtctccccc gtcgcgcctc cggcctccga tctctgcgcg aaggcggcgg
     9841 gtaaaatctg agttaatagt ataactgcaa tgaggagacg caggggagag agtgcgcggc
     9901 gcgtgtgagc aaaagacatc tttgaacttc ctttcatcaa accgggtcgc gattcggaca
     9961 accgacgagg ttgggaatgc aaacggtttt tctatgcgaa ttttgggagc aagtcaaacg
    10021 ctggaaacgt tgaactagaa tacagaaaac agaatacagg agtcagaaga aggagacgcg
    10081 cacgttttat tctgactgct ggattctgaa ttccgactcc tgcttttcat tcctcgtcca
    10141 tcgtcgcttt tctacccgcc gaaacttctg cggaacaggt aggccatctt gatgaagaag
    10201 gtgcgaccgt tgcggcggaa gcccggttcg agttggccgt tgatgggcga gaaggtgttg
    10261 atgttggcgt cgtcgttgta gccgacgtag aaggccgtgc cggggttcgg cgtccagccg
    10321 aggaggaact ggccgcgtgc gcgtcgcggg agcgtctcgt aatcgatgcg cgcccgcgcg
    10381 aagagaaagc gcgtgaactg gtaggtggtg cgccacgaga ggaggttgac atcgaaggcg
    10441 acgagatcgg tgtcgtagcg gcgcaggtgg ctcttggtgt agtcgagcgt cgagcgcagc
    10501 gcgttggtcg gctggtaggc gagcgtggcg cgcaactcga agaggccgcc gcgtcccgga
    10561 tcgagcggcg cgtggcagac cgccgggagc ggttcgtctt cctcatcgca cagacctcgc
    10621 gccgcggcct cgcgcgccgc gattgccacg gggctgacac gcgggaaacg tcgccctgcg
    10681 ccgaagtcgt aatcgaaatg gccgtcgcga tagagcgcgc gccagttgac cgaatatttt
    10741 ttgcttgggc gatagccgcc gaagaagaag tagtgattct tgcgcgaaga gcgttcggag
    10801 ctttcccccg cgaacgcgcc cgcgagtgtg gccgagcgac gcgcgccgaa ctcctcttcg
    10861 atcagacgct cgtaggcata ttcgtaagcc gcgccgatgt tgaactgccg ctggaagaag
    10921 aattgaaagt tggtttcgct ctcccaagcc tggctgcgtc cctgaaaatc gtggctgatg
    10981 aaagagaaat tatggacgtg ccagttgatg agtctggctt tcggtttcgg cgtcgtgttg
    11041 tagctggtg
//
LOCUS       DASWNS010000645         7759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_76605, whole genome shotgun sequence.
ACCESSION   DASWNS010000645 DASWNS010000000
VERSION     DASWNS010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76605"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VGB76_22345"
     CDS             <1..468
                     /locus_tag="VGB76_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101682.1"
                     /translation="LAQSKLPDSYFDLDRTELWQPTPATNEFSLLMDFIATLPFKATG
                     RMLIMYDDVQRPVPAHRDHTETDLCHEFIWFRTNMNKPFYMFNHQTHEKRYVESYSAW
                     FDTVNQFHGTDPGEGLTFSIRVDGIFNDRFRERIPTPASNVASTPALWAAVSG"
     gene            complement(500..1462)
                     /locus_tag="VGB76_22350"
     CDS             complement(500..1462)
                     /locus_tag="VGB76_22350"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0101683.1"
                     /translation="MKTSLLNQKRLPFVVEIDRDREAGSGAEASFAPFLDERDFLHRQ
                     LLEHGALLLRGLPAAAPAEFARFVREFSGKPLLDYAGGASPRVKLGDGVYTSTEYPRN
                     YTLSLHNELSYTARWPSHLFFCCVTPAASGGQTPLGDSRAILRSLDTKVVGEFKRREI
                     KYVRNLHGGAGSGYSWQEAFETDDKLAVERYCRAGGIDLKWRGDGGLTLTEVRPATTT
                     HPVTGEEVWFNQADGFHPSSIDAETYQLLISTMTEEEFRLNSYFGDGSPLDRSALEQI
                     RDVMRKETVLVEWQRGDVLIVDNLLAAHGRMPFTGQRKIILAMT"
     gene            1770..4100
                     /locus_tag="VGB76_22355"
     CDS             1770..4100
                     /locus_tag="VGB76_22355"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0101684.1"
                     /translation="MKIYISGLYSGTNPQPGIGIARSLRAAYPDANLVGVEYSNRCSG
                     IHWQDFDELWLQRPWEELNLDAYADNIREALDAGAYWISGVDLEIMWLASIFPEGHKN
                     LLTPSARALQKITKPAVEAHKGLPLSIPTFITTERSDWELHAFCRQHGWRVWLKGPYY
                     EAVRTPSWDVFERTRTLLSRAWSTERLFLQTHITGYEESVCFSAYQGEITGSVYMRKR
                     ELTEEGKTWAGDVTEVPNEFTEPLRKIVKELNWTGGAELEMVHDTGGKLWLLECNPRF
                     PAWIYGSTIAGHNLPALMLERATGIPAQSPQSACEEFTRVVLEVPVRSQFPLPSLPEP
                     FPGAIGHSMKHPSGTLALAERLHKLNIVESSSHGHSNGNGYSNGNGNGNGNGNGHHES
                     MDAVSTAPVVPSSFIKDLATHNFDEMETPSWLYFESTAASLFKRASDLASKLSTSTVE
                     VTNGYSLKTNPDERLIRLAKESGFLAEAISLLEVQKALKTGFKPEQVILNGPGKWWPQ
                     GLLPTSPIHAVFSDSLNDLKRVVASLEKGELQAKIVGVRLRAASSRFGIPVETPDALK
                     ALVEAIETLPRNSTFGVHFHMASSNIGVRQWWHLYESMLRWCRSIEALSGRRIECLDV
                     GGGWFPDDWQADSADQFAAAVGRARKFLPGVEQVISEPGKALAQPSMAVAMRLLEFEE
                     SAEGEIKAAVVDGSIAELPMHFFQPHRILHRDEQTGEWRALERGKTQLLGRLCMEHDI
                     VALNVELPKGAQVGDVLVFCDAGAYDRSMSYVFGRG"
     gene            4081..4770
                     /locus_tag="VGB76_22360"
     CDS             4081..4770
                     /locus_tag="VGB76_22360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101685.1"
                     /translation="MSLVEGNELIEVSETDFVDTDAAIEKWRHFPASAILHHGKMCCR
                     IAREWIFSFDYGQLNGEHPLTGPRWIRKKYTWGPSRWPISWCEAVEQETLDCGALASM
                     TQEVFAARGIKSYQAQFIQQYSEDSATHWQKKWNGEEISTHWINEDLIYHEGCAVVVR
                     DNEVRIWDSSAGWWVNPKQFGGYGGLLVLRICTAQTDAVDFNWGTHRIIPNQWQKIER
                     ARNHFASAAAG"
     gene            complement(4900..7377)
                     /locus_tag="VGB76_22365"
     CDS             complement(4900..7377)
                     /locus_tag="VGB76_22365"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY0101686.1"
                     /translation="MNTLNMHSSTHRRPLVALLTLTLLFQCGLGAFVTSAQTLPLKFE
                     VATVDAATGATKTGYYLGERVSAVFSFTNLSGAALTMPVAEQLEISVKLSGTINRSDR
                     PALYEGVRGGRGGAQVDPDGTVYFTGAPLIATTVAAGQKVVVRIDDLAGFFGSKLADG
                     SYTLSAVYGGSQQAQATFTVAIEEARSVPVLEQLAAGSDPDAKAWANSFLDLIRKPSI
                     NGRILVAEGLPLPGVTINVTGSDKTNIDTRADGNYELTHLTSGGTYTLTPSLEGYTFE
                     PAQRTYTGLTSKVTGANFNATRARAGVNVASEEAGAVATASSTLDEDFPVESVINGSK
                     TGIGWGQGSGGWNDATHGLFPDWVEVNFGAFKYINWINVFTLQDNFENPSDPTQAQTF
                     TLYGITDFDVQYWDGATWVNVPGGTVNANSNVWRRFDFPTIGTSKIRVYIRGAKDGHS
                     RITEIEAFHANAAPKIKFFGLYQGAPGSTFNFNYSGSDSDGFIARYEWDFGDNTTATG
                     PTQSHTYTNVGTYTVSLRVTDDGGESYTARTTVKIADPAKPPVASTGGTYQGSPGKDV
                     LFDGMASSDSDGTIASYAWNFGDGASGSGPTPAHAYAQAGTYNVSLTVTDNSGMTATA
                     TTTANITTQPTVATPTNLLATTNASGQVEITWSSTSPPHHYQIERATSLNGTFVALGA
                     TPTTNSHTDSSMSPNGVYFYRVCAVDASGNKSGYTNIDVAANYAFTDPALLAGVTFVK
                     AQHVTELRQAIDAMRLAVGLQPAAWTDAELSNVFIKAVHVLELRTNLDQALTQVGVPA
                     VTYTDPQLAIGYLIRKAHIEELRLRLN"
     gene            complement(7374..>7759)
                     /locus_tag="VGB76_22370"
     CDS             complement(7374..>7759)
                     /locus_tag="VGB76_22370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101687.1"
                     /translation="DAAGNFTGPNIDPNSEWTDLVANLPAGTIKHQTVQAFETNFGNL
                     KFAFVFLNQTATDARRRIVGRHEIGHASDHVLFGPATETDPKETDHWIRGLMHRTADQ
                     VPVTRPDGDDTFADDSILRLRGRKR"
BASE COUNT         1517 a         2207 c         2434 g         1601 t
ORIGIN      
        1 ctggctcaat caaagctgcc ggacagctat tttgatctcg acagaacgga actgtggcaa
       61 ccgactccgg ccaccaacga gttttcgctg ctgatggatt tcatcgcaac cctgcccttt
      121 aaagccaccg ggcgcatgct catcatgtat gacgacgtgc aaagacccgt tcccgcgcac
      181 agggatcata cggagactga tctctgccac gagttcatct ggttcagaac gaacatgaac
      241 aagcctttct acatgttcaa tcaccagacg cacgagaaac gctatgtcga atcctacagc
      301 gcgtggttcg atacggtgaa ccagtttcac ggaaccgatc cgggcgaggg actcactttc
      361 agtattcgtg tggacggaat tttcaacgac cgtttcagag aacgcatccc gacccctgcc
      421 tctaacgtgg cctcgacgcc cgccctctgg gctgctgtct caggctgaca gcaaggttcg
      481 gatacgggtc tggaaattct caagtcatgg cgagaataat ctttcgctgg ccggtgaaag
      541 gcatgcgccc gtgagcggcg agcaggttgt ccacgatcaa cacgtcgccc cgctgccact
      601 ctacaaggac agtctctttc cgcatcacgt cgcgtatctg ttcgagcgca gagcggtcga
      661 gcggtgagcc gtcgccgaag taagagttca agcgaaactc ttcttccgtc atggtcgaaa
      721 tcaggagctg gtaagtttcc gcatctatgc tgctcggatg aaacccgtca gcctgattga
      781 accagacttc ttctccagtg acggggtgtg tggtggtcgc ggggcgcacc tcagtcaaag
      841 tcaacccccc atcgcctctc catttgagat cgattccgcc cgcgcgacag taacgctcga
      901 ccgcgagttt gtcgtccgtc tcgaaagcct cttgccagga gtaaccggaa cccgcgccgc
      961 cgtgcaggtt gcgcacatac ttgatctctc tacgcttaaa ttcgccgacg actttcgtgt
     1021 ctaagcttct gaggatggcg cggctatcgc ccagaggcgt ttggccgccg ctcgcggcag
     1081 gcgtgacgca gcagaagaac aggtgtgaag gccagcgcgc cgtgtaggac aactcgttat
     1141 ggagggagag cgtgtagttg cgcgggtatt ccgtcgaagt atacactccg tcgcctaact
     1201 taacgcgcgg cgatgcgccc ccggcgtaat cgagcagtgg cttgcctgaa aactcgcgca
     1261 cgaagcgcgc aaactcggcg ggggcagcgg cagggagtcc gcgcaacagc agcgcgccgt
     1321 gctccaacag ttgacgatgc agaaaatctc tctcgtcgag aaatggtgca aaggacgctt
     1381 ccgcgcccga acctgcttcc cggtcgcggt caatctcgac gacaaagggc agtctcttct
     1441 gattgagtag gctcgttttc atgatttccc ccggttctgc ggcgcgagtt taataagttt
     1501 gatcaaaacg tttccgtacc ggaacgtaca gacaatacga gtgcgcgact gatatttatt
     1561 tattacaaaa attaactcac cgtccagacg ccggtgtttt aagcggcgga ctagagtatc
     1621 agagcaataa acctacgtgg tattgaagta atcattccca cgaaagtcta gctttgtgcg
     1681 cgtttaagcg cagacttcaa cgtttgtaaa aggtcttcgc caagcgggcg aggctgcgtt
     1741 ctatcagtgt ctaatttagg gggttagatt tgaaaattta catcagcggg ctgtacagcg
     1801 gtactaaccc acagcccggc atcggcatcg cacgctctct gcgcgcggca tatcctgacg
     1861 ccaacctcgt cggcgtcgaa tattctaatc gttgttcggg cattcactgg caagacttcg
     1921 acgagctatg gctgcaaaga ccttgggaag agttgaacct tgacgcttat gcagacaaca
     1981 ttagggaggc gctggatgcg ggggcgtact ggatttccgg cgtcgatctg gagatcatgt
     2041 ggctcgcctc gatctttccc gagggacaca agaacctgct gacgccttcg gcacgcgctc
     2101 tacaaaagat caccaagccc gccgtggagg cgcacaaggg tctgcccctg agcattccca
     2161 cgttcatcac caccgaacgc tcggattggg agttgcacgc tttctgccgc cagcatggct
     2221 ggcgggtctg gctcaagggt ccgtattacg aggccgtccg cacgcctagc tgggacgtgt
     2281 tcgagcgtac ccgcacgctg ctctcgcgcg cttggtcaac cgaacgcctc tttctccaaa
     2341 ctcacatcac gggctatgaa gagtcggtct gcttctcggc ttaccaggga gagattacgg
     2401 gcagcgtcta catgcgcaag cgcgaactga cggaagaggg taagacgtgg gcgggcgatg
     2461 tgacggaagt gcccaacgag ttcaccgagc cgctgcgcaa aattgttaag gaactgaact
     2521 ggactggcgg cgcggagttg gagatggtgc acgatacggg cgggaagtta tggctgctgg
     2581 agtgtaaccc gagattcccg gcctggattt acggctcgac catcgccgga cataatctgc
     2641 ccgcgctgat gctcgaacgt gcgacgggca ttccggcgca gtcgccgcag agcgcctgcg
     2701 aggagtttac gcgagttgtg ttggaagtcc ctgtgcgctc gcagtttccc ttgccgagtt
     2761 tgccggaacc ctttcccggc gcaatcggcc attcgatgaa gcacccttcg ggcacgctcg
     2821 cgctcgccga gcggctgcac aaactgaaca tcgtcgagag cagcagccac ggccatagca
     2881 acggcaacgg ctatagcaac ggcaacggca atggcaatgg gaacggtaac gggcatcatg
     2941 aaagcatgga cgccgtgagc accgccccgg tcgtgccgtc ttccttcatc aaggatctcg
     3001 caacgcacaa cttcgacgaa atggagacgc cttcgtggct ctatttcgaa agcactgccg
     3061 cttcgctctt caaacgcgcc tccgatttgg cgagcaagct cagtacctct acggtcgaag
     3121 ttacgaacgg ctacagcctc aagactaatc ccgacgagcg actgattagg ctggcgaagg
     3181 agtcgggttt tctggccgaa gcgatcagcc tgctcgaagt tcagaaagct ctcaagaccg
     3241 gcttcaagcc cgaacaggtg atcttgaacg gccccggcaa atggtggccg caaggtctgc
     3301 tgccaacctc gcctatccac gccgtcttca gcgattcgct taacgacctg aaacgggtgg
     3361 tcgccagtct ggagaagggg gaactgcaag ccaagatcgt cggcgtccgg cttcgcgccg
     3421 ccagttcgcg ctttggcatt ccggtggaaa cgcccgacgc gctcaaggct ctggttgaag
     3481 cgattgagac gttgccgcga aactcaactt tcggcgtcca ctttcacatg gcgagcagca
     3541 acatcggtgt ccgccagtgg tggcatctgt acgaatcgat gttgaggtgg tgtcgctcta
     3601 tcgaggcgct cagcgggcgg cggatcgaat gtctggacgt gggcggcggt tggtttccgg
     3661 atgactggca ggccgactcg gcagaccagt ttgccgccgc cgtcgggcgc gcgcgcaagt
     3721 ttctgcccgg cgtcgagcag gttatatccg aacccggcaa agcgttggcg cagccttcga
     3781 tggcggtggc gatgcgcttg ctggaattcg aagagtcggc ggaaggcgag atcaaagcgg
     3841 ctgtggttga cggctcaatc gcggaactgc cgatgcactt ctttcagccg cacaggattc
     3901 tgcaccgcga cgaacagacg ggcgagtggc gcgcgttgga gcgcggcaag acccaactct
     3961 tgggcaggct gtgtatggag cacgacatcg tggccttgaa tgtcgaactg ccgaaaggcg
     4021 cgcaggtcgg cgatgtctta gttttctgtg atgcgggagc ttacgatagg agtatgtctt
     4081 atgtctttgg tcgagggtaa cgaactaatc gaagtcagcg aaacggattt tgttgacacg
     4141 gacgccgcca tcgaaaaatg gcggcacttc ccggcatccg ccattctgca tcatggcaaa
     4201 atgtgctgcc gcatcgcccg cgagtggatc ttctctttcg actacgggca actgaacggc
     4261 gagcacccgc tcaccggacc acgctggatt cgtaaaaagt acacttgggg tccatcgcgc
     4321 tggccgatca gctggtgtga ggccgtcgaa caagagacgc tcgactgcgg cgcgctggcc
     4381 tcgatgacgc aagaggtttt cgcagcgcgc ggcatcaaga gctatcaggc acagtttata
     4441 cagcagtaca gtgaagattc agccacgcac tggcagaaga agtggaacgg cgaggagatc
     4501 tccacccact ggatcaacga ggacttaatc tatcacgaag gctgcgccgt cgtagtgcgt
     4561 gataacgagg tcaggatttg ggattcgagc gcgggctggt gggtcaaccc gaagcagttc
     4621 ggcggttatg gaggattgct ggtgctccgc atctgcaccg cgcagaccga cgcggtagac
     4681 tttaactggg gcacgcatcg catcattccc aaccagtggc agaaaatcga aagggcgcgc
     4741 aaccactttg cttcagccgc cgcgggctga aacgaaaggc gtccgtaacg gcgaagactg
     4801 cttcgcccag ccgggagcat gctcctgaaa ctaaagtccc ttcaacgatt gctcgttgaa
     4861 gggactttat gttttgcccg cgttggagcg tggcgggtgt taattaagtc gcaggcgtag
     4921 ctcttcgatg tgagctttcc tgatgagata gccgatggcg agttgagggt cggtgtaggt
     4981 gaccgccgga acgccgacct gtgtgagcgc ctgatcgaga ttggttctca actccagcac
     5041 atgcacggcc ttgataaaca cgttcgacag ttccgcatca gtccacgcgg cgggttgcag
     5101 cccgaccgcg aggcgcatgg cgtcaatcgc ctgacgcagt tcggtaacgt gttgcgcctt
     5161 gacgaaggtt acaccggcta agagcgcggg gtcggtgaag gcgtagttag cggcgacatc
     5221 aatgttggtg tagccggact tgttgccgga agcgtccacg gcgcagacgc ggtagaagta
     5281 gacgccgttg ggactcatgc tgctgtccgt gtggctgttg gtcgtcggcg ttgcgccgag
     5341 tgcgacgaaa gtaccgttga ggctcgtcgc acgctcgatc tggtaatggt gcggcgggct
     5401 ggtggacgac caagtgatct ctacctgtcc gctcgcgttg gtggtagcga gcaggttcgt
     5461 cggcgtggcg acggtcggtt gcgtggtgat gttggcggtc gttgtcgccg tggcggtcat
     5521 gccgctgttg tccgtgacgg tcagcgagac gttataggtt ccggcctgcg cgtaggcgtg
     5581 tgcaggcgta ggaccggagc cgctcgcgcc gtcgccgaag ttccaggcgt agctggcaat
     5641 cgtgccgtcg gagtcggaag aggccattcc gtcgaagagc acgtccttgc cgggagagcc
     5701 ttgatacgtc ccgccggtgg aggccacggg cggcttcgcc gggtcggcga tcttgacggt
     5761 cgtgcgggcg gtgtaagact cgccgccgtc gtccgtcacg cgcagcgaga cggtgtaggt
     5821 tcccacgttc gtgtaagtgt gcgactgcgt cggtccggtc gcggtcgtgt tgtcgccgaa
     5881 gtcccactcg tagcgtgcga tgaatccgtc ggaatcggag ccgctgtagt tgaagttgaa
     5941 ggtgctgccc ggcgcgcctt gatagagtcc gaaaaacttg atcttcggcg cggcgttggc
     6001 gtgaaacgct tcgatctccg tgatgcggct gtgtccatcc ttcgcgcccc ggatgtagac
     6061 cctgatcttg ctcgtgccga tggtcgggaa gtcgaacctg cgccagacgt tgctgttggc
     6121 gttgacggtt ccgccgggca cgttcaccca cgtcgcgccg tcccagtatt gcacgtcgaa
     6181 atccgtgatg ccgtagaggg tgaaggtttg cgcctgtgtc gggtcggacg ggttctcaaa
     6241 attgtcctgc aacgtgaaga cgttgatcca gttgatgtac ttgaacgcgc cgaagttgac
     6301 ctcgacccaa tcgggaaaga ggccgtgcgt ggcatcgttc cagccgccgc tgccctgacc
     6361 ccaaccgatc cccgtcttcg agccgttgat gacgctctcg acggggaagt cttcgtcgag
     6421 agtcgaagag gcggtggcga cggctccggc ctcttccgac gcgacgttga cgcccgcgcg
     6481 cgcgcgcgtc gcgttgaagt ttgcgccggt caccttggaa gtcagccccg tgtaagttct
     6541 ctgcgccggt tcgaaggtat agccttcgag cgagggcgtg agcgtgtagg tgccgcccga
     6601 agtgagatgc gtgagttcgt agttgccatc ggcgcgcgtg tcgatgttgg tcttatccga
     6661 gccggtgaca ttgatggtca cgccggggag gggcagccct tctgcgacga ggatgcgccc
     6721 gttgatggac ggcttgcgaa tcaggtcgag gaaggagtta gcccaggcct tcgcgtcggg
     6781 gtcggaaccg gcggcgagtt gttcgaggac ggggacgctg cgcgcctctt cgatagccac
     6841 cgtgaaagtg gcctgcgcct gctggctgcc gccatagacc gcgctcaggg tgtaggagcc
     6901 gtcggccagt tttgagccga agaagcccgc cagatcgtca attctgacga cgaccttctg
     6961 ccccgccgcg acggtcgtgg cgatgagcgg cgcacccgtg aagtagaccg tgccgtccgg
     7021 gtctacctgc gctccgccgc gcccgccgcg cacgccctcg tagagcgcgg ggcggtcgga
     7081 gcggttgatc gtgccagaga gtttgactga gatttccaac tgctcggcca ccggcatcgt
     7141 caatgccgcg ccgctcaggt tggtgaaaga gaagactgcc gaaacccgct cgccgaggta
     7201 gtagccggtc ttggtcgcgc ccgtggcggc atcaaccgtt gcgacctcga acttcagggg
     7261 aagagtctgc gccgaggtca cgaacgcccc cagcccgcac tggaagagca gtgtgagcgt
     7321 gagcagcgcg accaacggac gccggtgcgt cgaactgtgc atgtttaagg tattcatcgt
     7381 ttccgccccc gcagtcttaa gatgctgtca tcggcaaaag tgtcgtcgcc gtccggacgg
     7441 gtgacgggta cttgatcggc ggtgcggtgc atcagcccgc gaatccagtg atcagtctcc
     7501 ttcgggtcgg tttcagtggc cggaccgaag agcacgtggt cgctggcatg gccgatctca
     7561 tgccgcccga cgatccttct gcgcgcgtcg gtcgccgtct ggttgaggaa gacgaaggcg
     7621 aacttcagat tgccgaaatt cgtctcgaac gcctgcaccg tctgatgctt aatagtgccc
     7681 gcaggcaggt tcgctaccag gtcagtccac tcgctgttcg ggtcgatgtt gggtccggtg
     7741 aagttgcccg ccgcgtcct
//
LOCUS       DASWNS010000646         3600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_206967, whole genome shotgun sequence.
ACCESSION   DASWNS010000646 DASWNS010000000
VERSION     DASWNS010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206967"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VGB76_22375"
     CDS             complement(<1..696)
                     /locus_tag="VGB76_22375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101688.1"
                     /translation="MNKISAEDALARLSSTARQTLIQSEVSRMALVQFVSNYEDLSTD
                     RGYQFKFYCDKCGNGYMSRFQPSTIGTAGSLLRAAGDLFGGWASTAGNSAYDIQRSVG
                     GKAHDDALAKAVGEGKGHFHQCSRCGKWVCPEVCWNTSAGQCEDCAPDYQEELASSHA
                     NAKAEAARQQLHEKALQTDYASKIDMSADAYVKAPAQAQSSGGAEQALLCISCGVDVG
                     KAKFCPECGTPTKP"
     gene            complement(704..1555)
                     /gene="mazG"
                     /locus_tag="VGB76_22380"
     CDS             complement(704..1555)
                     /gene="mazG"
                     /locus_tag="VGB76_22380"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY0101689.1"
                     /translation="MPVTFNDLVALMATLRSPEGCPWDREQTYATLAPMLLEEAYEAF
                     EAVEEAREGRPEELRGELGDLLFQIVFYAQVASERGEFTIDDVVEAIHTKMVRRHPHV
                     FGDASAQDSAEVLRNWEAIKAEEKRAAGKSEAAGESSLLDGISAKAPALMEAHQLTTK
                     AARVGFDWQNIEQIFDKLHEEVDELKAAIEESVADDNAGDAAATSEAAQARVREELGD
                     LLFVVTNIARHLKVEPEAALKLTNRKFRRRFRHIERGLAARGQSLDAATLDEMERLWV
                     EAKSSDK"
     gene            1992..2123
                     /locus_tag="VGB76_22385"
     CDS             1992..2123
                     /locus_tag="VGB76_22385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101690.1"
                     /translation="MKFQAELQSRMERDTREYEEARQTLLKQAGDDERDRQNIEHAA"
     gene            2512..3114
                     /locus_tag="VGB76_22390"
     CDS             2512..3114
                     /locus_tag="VGB76_22390"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEY0101691.1"
                     /translation="MSEEKPHFKVTDRRLFNPDGTPRDVEREAEAASESAPATGATST
                     GAETETTAQTPAASSPPAVDTAARPEPSPPPPVETSREPDSAPMPSAPSGAAGAVDDP
                     TPFANLVMFIASPAAAAMGMTEHPGLAPGELDLPLAKHCIDLLGTIQQKTRGNLGVQE
                     GQILEGLLAELRMQYISLTSTQRAPAPPRGFTGSDITGGR"
     gene            3242..>3600
                     /locus_tag="VGB76_22395"
     CDS             3242..>3600
                     /locus_tag="VGB76_22395"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0101692.1"
                     /translation="MKFTLLGTGTSTGVPSIGCECETCTSDDARDKRLRVSVLVEHDG
                     QSVLIDTSSDFRQQALRQKLRRLDAVLITHCHADHIFGLDDIRPLNFRHGALGVYAND
                     RAWRDIRRIFQYIFEPS"
BASE COUNT          690 a         1132 c         1024 g          754 t
ORIGIN      
        1 cggcttcgtg ggcgtgccgc attccgggca gaacttggct ttgccgacat ccacgccgca
       61 agagatgcag agcagtgctt gctctgcgcc gccggagctt tgcgcctgcg cgggggcttt
      121 cacgtaagcg tctgcgctca tgtcgatctt cgaagcgtag tcggtctgca aggctttctc
      181 gtggagttgc tggcgcgcgg cttcggcttt ggcgttggcg tgtgaagagg cgagttcttc
      241 ctgatagtcc ggcgcgcaat cttcgcactg cccggcgctt gtgttccagc aaacttccgg
      301 gcagacccac ttgccgcagc gcgagcattg atggaaatgg cctttgccct ctccgaccgc
      361 tttggcgagc gcatcgtcgt gcgccttgcc gccgaccgaa cgctgtatgt cgtaagcgga
      421 atttccggcg gtgcttgccc agccgccgaa caggtcgcct gccgcgcgca acagcgaccc
      481 cgccgtgccg atggtcgaag gctgaaaacg cgacatgtaa ccgttgccgc acttgtcgca
      541 gtaaaacttg aattgataac cacggtcggt agacaggtct tcataattgc ttacgaactg
      601 caccaacgcc atgcgcgaaa cctccgattg tattagcgtc tgacgcgccg tagatgagag
      661 ccgcgcgagc gcgtcttccg ccgatatttt attcattcaa acttcacttg tcactgcttt
      721 tcgcttcgac ccagagcctc tccatttcgt cgagcgtggc ggcatcgagc gactggccgc
      781 gcgctgcgag gccgcgttcg atgtggcgaa agcgacgtcg gaatttgcgg ttcgtcaact
      841 tcaaagcggc ttcgggttcg accttcaaat ggcgtgcaat gttcgttacg acaaagagca
      901 ggtcgcccaa ctcttcgcgc acgcgcgcct gtgccgcctc gctcgtcgcg gcagcgtcgc
      961 ccgcgttatc gtccgcgacg ctctcttcga tagcggcttt gagttcgtca acctcttcgt
     1021 gcaacttgtc gaaaatctgt tcgatgttct gccagtcgaa accgacgcgc gccgctttcg
     1081 tcgtcagttg gtgcgcttcc atgagcgcgg gcgctttcgc ggagatgccg tcgagcagag
     1141 acgactcccc ggcggcctcg ctctttccgg cggcgcgttt ctcttcggcc ttgatcgctt
     1201 cccagttgcg caagacctcc gccgaatcct gtgcgctcgc gtcgccgaaa acgtgcgggt
     1261 ggcggcgcac catcttcgtg tggatcgctt ccaccacgtc gtcaatcgta aactcgccgc
     1321 gctccgaggc gacctgcgcg tagaagacga tctgaaacaa gaggtcgccg agttcgccgc
     1381 gcaactcttc cgggcgaccc tcgcgcgctt cttcgacggc ctcgaaagcc tcgtaggctt
     1441 cctccaacag catcggggcg agcgtggcgt aggtctgttc gcggtcccag gggcagccct
     1501 caggggagcg taaagtagcc atcaaggcga cgagatcgtt aaatgtgacg ggcatagatt
     1561 tgttcggcag cagtgcgcgg ttgaaaattc aaaacgtgtt gtcagaacgt cggtggcgca
     1621 agtctaaaac gtaaaagtct attgttccaa gccgggttgg ggagaattgt gttccggcga
     1681 gaagccgccc ggctgcttct cgccacgcag ttcgagtcgc ttgagacggc ggggcgatgc
     1741 ggcgatttcc gccgattttc actcgcttta atgtacgttg tcgggtggac aaagcgaagt
     1801 gcgaagttgt ttaattggtt ccattctcct caaaaacgta tgtaagggcg ggtcgggtta
     1861 tggtagtagc cgcgttcatt ttcgggatca ttattctatt tttctcgttc tgcttcctca
     1921 tactttatgt ccggagccat aagtccatgc gagaaaatca tagcactctg gcaagacagc
     1981 gggagcggca attgaagttt caagccgagc tacagtcgcg gatggagagg gacacacgcg
     2041 agtatgaaga agcgcgtcaa accctgctca agcaggcggg cgatgacgaa cgcgaccgcc
     2101 agaacataga gcacgccgcc tgacagcagc gcagactgtt cctccacgca cgggcaagcg
     2161 ttcgctatgt gaaaaccctg tctctctctc tacgcacgcg ccgtctgttc gcgcgtcccg
     2221 ccatacctta cgaacttatt tcaaaacgac gacgcgccgc acaaggctgc aagcctcctt
     2281 tttgcgtctc ccttgagaca ttgatcttca cgcggcaggc gcagagagac gaagaaaggc
     2341 ggcaggtctg cgcgcccttc gttcgcgctc gctgcgagtt ggtcttgaag cctttcttgc
     2401 ggctgcgtcg aggtcggtgc taccatcctt gagtaattac cagaccagaa attttactaa
     2461 tggatgcgcg gcacagtcag cagtccgccg tcctggaatt tgagcgacga gatgagcgaa
     2521 gaaaaaccac atttcaaagt tacagaccgg cgtttgttca acccggacgg cacgccccgc
     2581 gatgtcgagc gcgaggctga ggccgccagc gaaagcgcgc ccgctaccgg cgcaacttca
     2641 accggagcgg agacggagac gacggctcag actcctgcgg cctcttctcc gcccgctgtc
     2701 gacaccgccg cgcgaccgga gccttcgccg ccccctcccg tcgaaacgtc gcgtgagccg
     2761 gattccgccc cgatgccttc cgctccatcc ggcgcggcgg gcgcggtgga cgaccccacg
     2821 cccttcgcca atctcgtcat gttcatcgct tctccggcag cggcggcgat gggcatgacc
     2881 gagcaccccg gcctcgcccc cggcgaactc gatctgccgc tcgccaagca ttgcatcgac
     2941 ctgctcggca ccatccagca aaagacgcgc ggcaacctcg gcgtgcagga ggggcaaatt
     3001 ctcgaagggc tgctcgccga gttgcgcatg caatacatct cgctcacctc gacacaacgc
     3061 gcccccgcgc ccccgcgcgg cttcaccggc agcgacatca ccggcggaag atgagaagaa
     3121 accagatcgt aaatcgtgaa gcgtaaatcg tgaatagagc aaacctgtct tcgtctattt
     3181 acaatttacg cttcacgatt tacggctttt gaatctattt acgattcacg attcacaagc
     3241 catgaaattc actctgctcg gcacaggcac ttcgacgggc gtgccctcca tcggttgcga
     3301 atgtgagacg tgcacgtccg acgatgcgcg cgacaaacgc ctgcgcgtct ccgtgctcgt
     3361 cgagcacgac gggcagagcg tcctgattga cacttcctcc gactttcgcc agcaggctct
     3421 ccggcaaaaa ttgagacgac tcgacgccgt gctcatcaca cactgtcacg ccgaccacat
     3481 cttcggcctc gacgacatac gcccgctcaa ttttcgccac ggcgcgctcg gcgtctatgc
     3541 caacgaccgc gcctggcgcg acatccggcg catcttccag tacatcttcg agccgtcgca
//
LOCUS       DASWNS010000647         6242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_258480, whole genome shotgun sequence.
ACCESSION   DASWNS010000647 DASWNS010000000
VERSION     DASWNS010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258480"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="VGB76_22400"
     CDS             <1..1361
                     /locus_tag="VGB76_22400"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0101693.1"
                     /translation="DKDSLRRVLRLTLEHAGYTVTECADAREAASEIARAPYRLVLTD
                     LRMPHGSGLDVLHAARAADADVPVVVMTAYGSIDEAVQAMKDGAHDFLQKPVDSNHLL
                     LLIGRALEQARLRTENILLREEWSRRYGFPRIIGESDAIKRAVAETQRVATTEATVLL
                     LGESGTGKELFARAVHHLSHRRDQPFVAINCAAIPETLIESELFGHERGAFTGATERR
                     PGKFELATGGTVFLDEIGELPLAVQGKLLRAIEEKTVDRIGGRMPVQVDVRIVAATNR
                     ELQQAAEAGEFRRDLYFRLAVFPVVIPPLRARGDDVVLLARHFAAQFGRELRGREATL
                     TEESLAALQTHDWPGNVRELENAIERACILADASVLDPRDLSLPVARAGENSAHTPET
                     FAALDLSGSLVEATERAVSFVERRKIAEALSAHDGNKTRAAETLGVSYKTLLTKIKDY
                     EL"
     gene            1403..3253
                     /gene="murJ"
                     /locus_tag="VGB76_22405"
     CDS             1403..3253
                     /gene="murJ"
                     /locus_tag="VGB76_22405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY0101694.1"
                     /translation="MPPQPDDEQTPSEVKPGSSARSTRDAEETQNPPPANEPHPGQQR
                     FQEAANLREVGERQDAGEIAGETVQSIGASTGEFPGEQVDAARERETEGTTGKYAFLV
                     GAGILLSRIVGLIRQRVFAYYFGNSAAKDAFDAAFKIPNFLQNVFGEGALSASFIPVY
                     ANLLAHRDEREAGRVAGAVFALLALVTSILVLIGVLTTPYLISLIAYGFEGERREWTI
                     RLVKIFFPGAGLLVLSAWCLGVLNSHRRFFLSYTAPVIWNLTIIAALVYFGGRVGEFP
                     LAEYAAWASVLGSAFQFAVQLPTVLRLVKHLRPAFDTTNVHVRKVARNFLPVFVSRGV
                     IQISAFIDQNIASLLPTGAVAALSYTQSLYTLPVSLFGMSVSAAELPAMSSAVGNAEE
                     VAAHLRRRLDAGLRQIAFFIVPSAMAFLALGDVMAAALYQTGKFGRADALYVWGILAG
                     SSVGLLASTLGRLYSSTYYALHDTRTPLRYAVLRIVSSVILGLLLAIYVPPALGIDAR
                     WGVAGLTIASGIAGWVEFYFLRRTLNGRIGQTGLPFAYISKLWLAALLSAIFAWGIKL
                     LIGSRHPILLAALILTPYGLLYFAITSLFKLPEAHTVVGRFTRILRRVRK"
     gene            3540..4223
                     /locus_tag="VGB76_22410"
     CDS             3540..4223
                     /locus_tag="VGB76_22410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0101695.1"
                     /translation="MIRRMLLTSVAVMIVALGVGAQSAHADSLTLVAGTFLTGSNSVT
                     GNVTVTITDIGSTGNVRVTIANNTNGDLTNLALNYNTAVTGPITITNVSGTGPSPTSL
                     TFGTNNVNAPGGARLDVNFGFPTGQPGGRLNAGETVSFNIDAASALLASGFNTLSVGG
                     QQNFYIIAHIQAIAGSGDSAKLIGGPGNPTQPVPEPATMILLGTGLAGIAAKMRRKRK
                     IANNDENEA"
     gene            4267..4998
                     /locus_tag="VGB76_22415"
     CDS             4267..4998
                     /locus_tag="VGB76_22415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0101696.1"
                     /translation="MRHLFSKLTFSLIGLLFFSLSAGSAYADGLVLLGTDPQHPCGVG
                     RGCGSARAPHVLSIQNHTTATGAVGRSVSSPNTDVMTGDWTRGSNTQTVSLTQIGATQ
                     ASQIRIYFDIIEPSAGTKSDVTLNSLVLTAFNTSGAEVFRAELVSPGQNFQMVGNGQG
                     TSDYTFGLDAAAAARLQAAIAANPNLRLGLIASVSRADGGPESFFIGAEGNPPAAVPE
                     PATMILLGTGLAGVAAKMRRKRKTD"
     gene            5488..6117
                     /locus_tag="VGB76_22420"
     CDS             5488..6117
                     /locus_tag="VGB76_22420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0101697.1"
                     /translation="MKIFATMLKPFAMSIALLAIFAAGQTIAKADPVTVSGTSTGTIT
                     GSVASYLSFTGNTFTATTAGGVGSFSGADRIGTFTLLSTPTDTPVNGAFTLNLTFTAP
                     SGINGGQAATYTATVSGVISTPNVGGVQIVFDNPIQTFTFANASGSGSFSIELPKIFV
                     QSGDTANLTAGLTGNQVAAVPEPATMILLGTGLAGVAARIRKRRKGEAS"
BASE COUNT         1226 a         2024 c         1747 g         1245 t
ORIGIN      
        1 aagacaaaga cagtctgcgc cgcgtgctgc gtctgacgtt ggagcacgcg ggctacaccg
       61 tcaccgaatg cgccgacgcg cgcgaggccg cgtcggagat cgcgcgtgcg ccctatcgtc
      121 tcgtgctcac ggatttgcgt atgccgcacg gttcgggact cgacgtgctg catgctgcac
      181 gcgccgcaga cgcggacgtg cccgtcgtcg tgatgaccgc ttacggctcg attgacgagg
      241 ccgtgcaggc gatgaaagac ggcgcgcacg actttctcca gaagcccgtc gattcaaacc
      301 acctcctgct gctcatcggg cgcgcgctcg aacaggcgcg cctgcgcaca gagaatatac
      361 tgctgcgcga agagtggtcg cgccgctacg gtttcccgcg catcatcggc gaatccgacg
      421 cgatcaaacg cgcggtcgcg gagacgcaac gcgtcgccac gacggaagcc acagtcctcc
      481 tgctcggcga atccggcacg ggcaaagaac tcttcgcacg cgccgtccac catttgagcc
      541 atcgccgcga tcaacctttc gtcgccatca actgcgccgc cattcccgaa accctgatcg
      601 agtcggaact cttcggccac gaacgcggcg cgttcaccgg cgcgaccgaa cgccgccccg
      661 gaaaattcga actcgccacg ggcggcacgg tcttcctcga cgagatcggc gaactcccgc
      721 tcgcagtgca gggcaaactt ctgcgcgcca tcgaagagaa gacggtggac cgcatcggcg
      781 ggcgcatgcc cgtgcaggtg gacgtgcgca tcgtcgccgc gacgaaccgc gaactgcaac
      841 aggcggcaga ggcgggcgag tttcgccgcg acctctattt ccggctcgcg gtcttccccg
      901 tcgtcattcc gcccctgcgc gcacgcggcg atgacgtggt gctgctcgcg cgccacttcg
      961 ccgcgcagtt cggcagagag ttgcgcgggc gcgaagccac gctcacggag gagtcgctcg
     1021 ccgccttgca gacgcacgac tggccgggca acgtgcgcga gttggagaac gccatcgagc
     1081 gcgcctgcat cctcgcagac gcttccgtgc tcgacccgcg cgacctcagc ctccccgtcg
     1141 cgcgcgcagg cgaaaactct gcccacacgc cggagacgtt tgccgcgctc gatctatccg
     1201 gctcgctcgt ggaagcgacc gagcgcgccg tcagtttcgt cgagcggcgc aagatcgccg
     1261 aagctctcag cgcgcacgac ggcaacaaaa cacgtgccgc cgaaacactc ggcgtcagtt
     1321 acaaaacgct tctgaccaag attaaagatt acgagttata aagactgcga gttataagac
     1381 tgcgagttat aaaccaccca agatgccgcc acagcccgac gacgaacaga caccatccga
     1441 agttaaaccc ggcagcagcg cgcgctccac acgcgacgca gaggagactc aaaaccctcc
     1501 gcccgcaaac gagccgcatc ccggacagca gcgatttcaa gaagcggcga acctgcgcga
     1561 ggtcggcgaa cggcaggacg caggcgagat cgcgggcgag actgtgcagt ctatcggagc
     1621 gtcgacgggc gagtttcccg gcgagcaggt tgatgctgca cgcgaacgcg aaacggaggg
     1681 cacgacgggc aagtatgctt tcctcgtcgg cgcgggcatc ctcctcagtc gcatcgtcgg
     1741 actcatccgc cagagagtct tcgcttacta cttcggcaac tccgccgcca aagacgcctt
     1801 cgacgccgcc ttcaaaattc cgaatttcct ccagaacgtc ttcggcgaag gcgcgctctc
     1861 cgcgtccttc atccccgtct acgccaacct gctcgcgcac agagacgaac gggaagcagg
     1921 ccgcgtcgcc ggagcagtct tcgcgctcct cgcgctcgtc acttcgattc tcgtcctcat
     1981 cggagtcttg accacgccct atctcatcag tctcatcgcc tatggcttcg agggcgaacg
     2041 acgcgaatgg acgatccggc tcgtcaagat atttttcccc ggcgcgggct tgctcgtgct
     2101 ctccgcctgg tgtctcggcg tgctcaacag ccaccgccgc ttctttctct cctacaccgc
     2161 gcccgttatc tggaatttga cgatcatcgc cgcgctcgtc tatttcggcg ggcgcgtcgg
     2221 ggagtttccg ttggccgaat atgccgcgtg ggcttccgtg ctcgggagcg cgttccagtt
     2281 cgccgtccaa ctcccgaccg tgctccggct cgtcaaacac ctgcgccccg ccttcgacac
     2341 gacgaacgtg cacgtccgca aggtggcgcg caacttcctg ccggtcttcg tcagtcgcgg
     2401 cgtgatccag atcagcgcgt tcatagatca gaacatcgcc agcctgttgc cgaccggcgc
     2461 ggtcgccgca ctcagctaca cgcagagtct ctacacgctg cccgtcagcc tcttcggcat
     2521 gtccgtctcc gccgccgaac tgcccgcgat gtcgagcgca gtgggcaacg cggaagaggt
     2581 cgccgcgcac ctgcgtcgcc gtctcgacgc gggcttgcgc cagatcgctt tcttcatcgt
     2641 gccctccgcg atggccttcc tcgccctcgg cgacgtgatg gccgccgcgc tctaccagac
     2701 gggaaagttc ggacgcgccg acgcgctcta cgtctggggc atcctcgcag gctcctccgt
     2761 cgggctgctc gcctccaccc tcggcaggct ctactcttcc acttactacg ccctccacga
     2821 cacgcgcacg cccctgcgct acgccgtgct gcggatcgtc tcatccgtca tcctcggcct
     2881 cctgctcgcg atttacgtcc cgcccgcgct cggcatcgat gcgcgctggg gcgtcgccgg
     2941 gttgaccatc gcttccggca tagccggttg ggtcgagttt tacttcctgc ggcgcacact
     3001 caacgggcgc atcgggcaga ctggcttacc cttcgcttac atctcaaaac tctggctcgc
     3061 cgcactcctg agcgcaatct tcgcctgggg catcaagcta ctcatcggct cgcgccaccc
     3121 catcctgctc gccgcgctga ttctcacgcc ttacgggctt ctctatttcg ccatcacctc
     3181 cctgttcaaa ttgccggaag cgcacacggt cgtcggacgt ttcacaagga ttttgcgccg
     3241 cgtccgcaaa taaagcgcgt atttcaaggg gaaactgcgt cggactctct ttcacagggc
     3301 gtccgtcgct cgttgcgccc cggttcacac tctccgcgcc tgtaagttta ttaagatttc
     3361 attctatttt tcggaaactg acggctatta tgggtttcct tacacacaga gaagcccaaa
     3421 caacctcgat acgtagtggc tcacatgcct taactatgcg ctggaagttt acttcttttc
     3481 aggttttaca gggatattgt agttgacaga ttcaaaccaa aggccgggag agagaagata
     3541 tgattcgcag aatgttactt accagtgttg ccgttatgat cgtcgctttg ggtgtggggg
     3601 cgcaatccgc ccacgccgat agcttaaccc ttgtcgccgg gacgtttctc acgggaagca
     3661 attccgtcac aggcaatgtg accgtgacca tcacggacat tggctcgacc ggcaatgtca
     3721 gggtcaccat agctaataac accaatggcg acttaaccaa tttggcgctc aactacaata
     3781 cagccgtcac cgggccgatc acgatcacca acgtctccgg gacaggccct tcgccgacga
     3841 gcctgacatt cggcaccaat aacgtgaatg cgccgggcgg cgcgaggctc gatgtgaact
     3901 ttggtttccc gacgggacaa ccgggaggcc gtctgaatgc aggcgagact gtctcgttca
     3961 atatagatgc cgcctccgcc ctcttagcct ccggcttcaa cacgctcagc gtgggcggcc
     4021 agcagaattt ttatatcatc gcgcacattc aggcaatcgc cggtagtggc gacagcgcga
     4081 agctgatcgg aggcccaggc aatcccactc agcccgtgcc cgaacctgcg acgatgatct
     4141 tgctcggcac cggccttgca ggcatcgcgg cgaagatgcg tcgcaaacgc aagatcgcca
     4201 acaacgacga gaacgaagcg taaacgttgc ggttgtaaag aatcttcaag gtaaagggaa
     4261 agaatcatgc gacacctctt ctcaaaattg acgttcagcc ttatcgggct actctttttc
     4321 agtctcagcg cgggcagcgc gtatgcagac ggcctcgtgt tgctcggcac tgacccgcaa
     4381 cacccgtgcg gcgtgggacg cggttgcggc agcgctcgtg caccgcatgt cctctcaatt
     4441 caaaatcaca cgaccgccac gggcgcggtc ggcaggtcgg tgagttctcc gaacacggac
     4501 gtaatgaccg gagactggac gcgaggcagc aacacgcaga cggtcagcct cacgcaaatc
     4561 ggggcgactc aagccagcca gatacgcatc tacttcgaca tcatcgagcc gagcgctgga
     4621 accaaaagcg atgtcacgct caattccctg gttctgaccg ccttcaacac gtccggagcc
     4681 gaagttttca gagccgagct tgtcagtccg ggtcagaatt tccagatggt cggaaatggt
     4741 cagggcactt cggattacac gttcggactc gatgcggcag cggcggcgcg attgcaggcc
     4801 gccatcgcgg ccaacccgaa tctgcgcctc ggattgattg ccagtgtttc gcgtgcggat
     4861 ggcgggccgg agagtttctt catcggtgca gagggaaatc cgcctgcggc tgtgcccgaa
     4921 cctgcgacga tgatcttgct cggcaccggc cttgcgggcg tcgcggcgaa gatgcgtcgc
     4981 aaacgcaaaa cggactaaga ttcagcctcc aaataccatc agagcatctg tcacgacgtt
     5041 agcgagcgcg ttaatttcgc tcccgtctct agctggaaga ataaacgggc gagccgcatt
     5101 caagcagtgc ggctcgcctg tttttttaag ttcaaccggg aatttttctg gaagtggccg
     5161 cgagatattc ctggaagtat ttgagaattt tctggaagta cttccgtatt gagtctttaa
     5221 acgcagtcaa aggattgaaa attgaagtat ttggcggtat aagggctgcg catcctcaac
     5281 cgtctggaaa gacaaatcaa gggtttttgc tccgcccctg attcttcgag agtcggcgtc
     5341 caaagtgcgg ttcggcgatg ctctcctacg cttcagcgcg tttttgtccc tgtgccggac
     5401 ggctttccga agtcgttttt gaggccggag gacgttgtga tttagttcaa atttccgtaa
     5461 aagggtccac ggaggcagag ttacaccatg aaaattttcg ctacgatgct caaaccattc
     5521 gccatgagca ttgctctact cgccattttc gccgccggtc aaacaatcgc taaagccgat
     5581 ccggtgacgg tttcgggaac ttcgacagga acgatcaccg gctcggtcgc tagttacctg
     5641 tcttttaccg gcaacacctt caccgcgacg accgctggcg gcgtcggttc gttcagcggc
     5701 gctgatcgca tcggcacttt cacgctgttg tctaccccga ctgacacgcc tgtgaacggt
     5761 gctttcactc tcaatctaac cttcaccgcc ccgtcgggaa tcaacggcgg ccaagcggcg
     5821 acatacaccg cgaccgtgtc gggcgttatc tcgacgccca acgtcggcgg cgttcagatc
     5881 gtgttcgaca acccgattca aaccttcacg ttcgctaacg cgagcggaag cggttccttc
     5941 tctatcgaac tgccgaagat tttcgtccag tccggcgata cggccaactt aaccgctggt
     6001 ttaaccggca atcaggtggc ggcggtgccc gaaccggcta cgatgatcct gctcggcacg
     6061 ggtctggccg gagtggctgc acgcatccgc aaacggcgta aaggggaagc gagctagttt
     6121 tcccaaataa cgcactgcgc ctgaaagaaa ccttaaaact ctcctcttcg gttgcaggcc
     6181 aagcgagccg gattagtttc gactaatccg gctcgttgct attttgcgcg cccattttgc
     6241 cc
//
LOCUS       DASWNS010000648         5136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_102578, whole genome shotgun sequence.
ACCESSION   DASWNS010000648 DASWNS010000000
VERSION     DASWNS010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102578"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VGB76_22425"
     CDS             <1..938
                     /locus_tag="VGB76_22425"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0101698.1"
                     /translation="PHVAEIAPDTIVIMVSGMQTVESAIEALRAGAFDYVMKPFDLRH
                     VEAAVSRALEHKRLGEAKRRYENYLEELVAERTAELDRTLATLENSYRMTLKALAAAL
                     ETRDRETHGHSERVVSFSLRLGRELNLTGDELRALEFGALLHDIGKIGVPDAILHKPA
                     QLCEAEWVRMRRHPMHGQQILQGIEFLEGAARVVGQHHEKWDGTGYPLGLRGTQIDLN
                     ARIFAVADAFDAITSDRVYRAGRPYDVAVAELEAYAGRQFDPAVVAAFRSIPREEWEE
                     LRATSFREWQERQDWQAAKLGARADVETRPGALVN"
     gene            complement(1111..1572)
                     /locus_tag="VGB76_22430"
     CDS             complement(1111..1572)
                     /locus_tag="VGB76_22430"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY0101699.1"
                     /translation="MDAITLLKTDHEKVDAIFAQLEETTERAQKTREELFTKLKQELE
                     LHTHVEEKIFYPAIKEAEETREITLEAFQEHHVVKVLLREMDAMEVTSETWTAKLKVL
                     QENVEHHVEEEEDEMFKKARTVLSTEQLEELGTLIEQEKQEQKPRTASANS"
     gene            1789..3327
                     /gene="ffh"
                     /locus_tag="VGB76_22435"
     CDS             1789..3327
                     /gene="ffh"
                     /locus_tag="VGB76_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY0101700.1"
                     /translation="MFESLSDKLKRTLKNLRGEGVLTPEHVDAALREIRLALLEADVN
                     YKVAKEFIAAVKEKAEGQQVWQELKPSEQVVKIVYDELVELMGGQSSRLVFTKTLPNV
                     VMIVGLQGSGKTTSTGKIALWLSKNQERKPLLVSTDVNRPAAREQLKVIARATGQSIF
                     EMPESNNPVELAREAYRHAQQTGYDTLLIDTAGRLHIDEELMDELKQIKTETHPVEIL
                     FVADAMTGQDAVKSGEQFHAQVGITGVVLTKMDGDARGGAALSIKQVTGQPVKFVGVG
                     EKYDALEPFYPDRIAQRILGMGDVLSLIEQVQSQVDQSEAEAQLKKLQKNEFTLDDFR
                     SQLRQVKKLGSFSKIMKLLPDQLLGGMGMPDLDEEQSAQMEKELKRTEAIIDSMTREE
                     RNDHRILNASRRRRIARGSGVNVSEVNALIKQYVEMRGMMKQLSGAGLFGGGSGGGGG
                     MKGRMMRKLGGMMGMPDMSGLMGGGGVGGDEGGMNLPALPPAGAGGNGRKKLRKKPRH
                     KKRK"
     gene            3423..3674
                     /gene="rpsP"
                     /locus_tag="VGB76_22440"
     CDS             3423..3674
                     /gene="rpsP"
                     /locus_tag="VGB76_22440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY0101701.1"
                     /translation="MLAIRLMRMGAKKSPSYRVVVKEKLSKRDGAYLENVGTYNPTRN
                     PAEVKLNLERITYWMERGAQPTDTVRQLIKSQSKPQAEV"
     gene            3757..3993
                     /locus_tag="VGB76_22445"
     CDS             3757..3993
                     /locus_tag="VGB76_22445"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY0101702.1"
                     /translation="MRDTVEMIVKTLVDDAEAVDVREELGRDQRSVIIEVRVAPDDMG
                     KLIGRQGRTVRALRSLFHAVSLKQGRRYILEIVE"
     gene            3990..4565
                     /gene="rimM"
                     /locus_tag="VGB76_22450"
     CDS             3990..4565
                     /gene="rimM"
                     /locus_tag="VGB76_22450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY0101703.1"
                     /translation="MSEDETLEGAEVEGDGEEELIAVARIAKVRGIRGEVAADLLTDF
                     PERFDGLEELISLNAAGERATLSIEKSWLHGGRVILKFAGYDSPEAAQALVGRELAVP
                     EAEAVELEEDEFYDWELIDCRVETIDNRRIGSVREVLHTGAAPVLVVRDETAAAAGRE
                     HLIPLAESICVEIDTEAKLIRVDPPEGLLEL"
     gene            4648..>5136
                     /locus_tag="VGB76_22455"
     CDS             4648..>5136
                     /locus_tag="VGB76_22455"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY0101704.1"
                     /translation="MRFDIITIFPDYFRTVFDYGIVRRAGASGLVEIEAHDLRAWAFD
                     KHRMVDDRPFGGGDGMVLKPEPLFAAVESLTGASEARQLAGTGTRVVMLSPQGRVLSQ
                     QVAAELAEAKQLVLICGRYEGVDERVVEMLATDEISIGDYVLSGGEPAAVVLVDAIVR
                     LIP"
BASE COUNT         1056 a         1412 c         1684 g          984 t
ORIGIN      
        1 tcccgcacgt ggccgagatc gcgcccgaca cgattgtcat tatggtgagc ggcatgcaga
       61 cggtggagag cgcgattgag gctctgcgcg ccggagcgtt cgactacgtg atgaagcctt
      121 tcgacctgcg ccacgtcgaa gccgccgtga gccgcgcgct cgaacacaaa cgattgggcg
      181 aagccaagcg tcgttatgaa aattatctgg aagagttggt ggccgaacgc acggcggaac
      241 tcgaccgcac gctggcgacg ctcgaaaatt cataccgcat gaccttgaaa gctctcgccg
      301 ccgcgctgga gacgcgcgac cgtgagacgc acgggcattc ggagcgcgtc gtcagtttca
      361 gtctgcgtct ggggcgcgag ttgaatttga cgggcgatga actgcgcgcg ctggagtttg
      421 gcgcgctcct gcacgacatc gggaagatcg gcgtgccgga cgccatcctg cacaagccgg
      481 cgcaactctg cgaagccgaa tgggtgcgga tgcgtcgcca tcccatgcac gggcagcaga
      541 ttttacaggg catcgaattt ctggaaggcg cggcgcgcgt cgtcggccag catcacgaga
      601 agtgggatgg gacgggctac ccgctcggct tgcgcggcac gcagatcgac ctcaacgcgc
      661 gcatcttcgc cgtcgcggat gccttcgacg cgatcacgag cgaccgcgtc tatcgcgccg
      721 gacgccccta tgacgttgcc gtcgcggagt tggaagcgta cgccgggagg cagttcgacc
      781 cggcggtggt ggcggcgttc cggagcattc cgcgcgagga gtgggaagag ttgcgcgcaa
      841 cctcttttcg cgaatggcag gagcggcagg actggcaggc ggcgaaactt ggcgcgcggg
      901 cggatgtgga gacgcgtccc ggcgcgctcg tcaactgaac gggagataag gataggaacg
      961 tgacgggcgt tgaagacccg gactgaaaaa gcggcgaata tttgaaccgg ctcaaaatcg
     1021 agtcggctac aaagtgagtc ggccacaaag taaggagcgt gcgcggtaga gttaactacc
     1081 gcgcacgctt ttgctttccg gtcgctgcgt tcaactattg gccgaggccg tgcgcggttt
     1141 ctgttcctgc ttctcctgct cgatgagcgt gccgagttct tcgagttgct ccgtgctcaa
     1201 gacggtgcgc gctttcttga acatctcgtc ttcctcttcc tccacgtgat gttcgacgtt
     1261 ctcttgcagc actttcaact tcgccgtcca cgtctcgctc gtcacctcca tcgcgtccat
     1321 ctcgcgcagc agcaccttga cgacgtggtg ctcctgaaag gcttccagag tgatctcgcg
     1381 cgtctcctcc gcttccttga tcgccgggta gaagatcttc tcttcgacgt gtgtgtgcag
     1441 ttccaactcc tgcttcaatt tcgtgaacaa ctcttcacgc gtcttttgcg cgcgctcggt
     1501 cgtctcttcg agttgcgcaa aaatcgcgtc tactttttcg tgatcagttt tcaacagtgt
     1561 gatcgcgtcc atctttcgtc ctccgtgtag tctgcccctg tgtcgtttgt acggtaaccg
     1621 tgcgtggcgg cgttcgattc attgatccat caagcgcagt gccagagagg aaagcggacg
     1681 cgccccgcgc gcaggccgaa aggcgtctcc gctcgtcctt gactctccct gcacacgcct
     1741 ttatcatgtc agttttcggc tacagcctgt ggccgcgtct gaggcatcat gttcgagtct
     1801 ttatccgaca aactaaagcg cacgctcaag aatttgcgcg gcgagggcgt gttgacgccg
     1861 gagcacgttg acgcggctct gcgcgaaata cggctcgcgc tgctcgaagc cgacgttaat
     1921 tacaaggtcg ccaaggaatt tatcgcggcg gtcaaggaga aggccgaagg gcagcaggtt
     1981 tggcaggaac ttaagccttc cgaacaggtc gtcaagatcg tctacgacga actcgtcgaa
     2041 ttgatgggcg gccagtcttc gcgtctcgtc ttcacgaaga ccctgccgaa cgtcgtgatg
     2101 atcgtcggcc tgcaaggttc gggcaagacg acttcgacgg gcaagatcgc gctctggctc
     2161 tccaagaatc aggaacgcaa gccgctactg gtttcgacgg acgtgaatcg tccggcggcg
     2221 cgcgagcagt tgaaagtgat cgcgcgtgcg acggggcagt cgatctttga gatgccggag
     2281 tcgaacaacc ctgtggaact ggcgcgtgag gcttaccggc acgcgcagca gacaggttac
     2341 gacacgctcc tgattgacac cgccggacga ctccacatcg acgaagagtt gatggacgag
     2401 ttgaagcaga tcaagacgga gacgcacccg gtcgaaattc tgttcgtcgc cgacgcgatg
     2461 acgggacagg acgcggttaa atcgggcgag cagtttcacg cgcaggtcgg catcacgggc
     2521 gtcgtcctga cgaagatgga cggcgatgcg cgcggcggcg cggcgctctc gatcaaacag
     2581 gtgacggggc agccggtgaa gttcgtcggc gtcggcgaaa aatacgacgc gctcgaaccg
     2641 ttctatccgg atcgcatcgc gcagcgcatt ctcggcatgg gcgacgtgct ctcgctcatc
     2701 gagcaggtac agtcgcaggt tgaccagtcg gaagccgaag cgcaactcaa gaaactgcaa
     2761 aagaacgagt ttacgctcga cgattttcgc agccagttgc ggcaggtgaa aaagctcggc
     2821 tccttctcga agatcatgaa actgctgccc gaccaattgc tcggcggcat gggcatgcca
     2881 gacctggacg aagaacagtc cgcgcagatg gaaaaggaac tcaagcgcac agaggcgatc
     2941 atcgactcga tgacgcgcga ggagcgcaac gatcatcgca tcctgaatgc gagtcgcagg
     3001 cggcgcatcg cgcggggttc gggcgtcaac gtctcggaag tcaacgcgtt gatcaaacag
     3061 tacgtcgaga tgcgcgggat gatgaagcag ttgtcgggcg cagggttgtt cggcggcgga
     3121 agcggtggtg gcggcggcat gaaaggccgc atgatgcgca aactcggcgg catgatgggc
     3181 atgcccgaca tgagcgggct gatgggcggc ggaggtgtcg gcggcgatga aggcggcatg
     3241 aatctgcccg cgcttccccc cgccggagca ggcggcaacg ggcgcaagaa actgcgcaag
     3301 aaaccgcgac acaagaaacg aaagtgacag gcgacaagtg acgagtgaca agataaaact
     3361 tttcttgttc ttgcctgtca cctgtcactt gtcacctgtc actaaatctg gaggatagaa
     3421 cgttgttagc aatcagattg atgcgcatgg gcgcgaagaa aagcccttcg tatcgcgtgg
     3481 tcgtcaaaga gaaattgtcg aaacgcgacg gggcgtacct tgagaatgtc ggaacctaca
     3541 acccgacgcg caacccggcg gaagtgaaac tcaaccttga gcggatcacc tactggatgg
     3601 agcgcggcgc gcagcccacc gataccgtgc gccagttgat caagagccag tcgaagccgc
     3661 aggcggaagt ttagtcttaa gtcccgaagt ctcaggtcgc aggtctgtca aggccgcccg
     3721 gcttgagatt tgggacttga ggcttcggga ctttgtatga gagacaccgt cgaaatgatc
     3781 gtcaaaactc tcgtggacga cgccgaggcg gtggacgtgc gcgaggagtt ggggcgcgat
     3841 caaagaagcg tgatcatcga ggtgcgcgtt gcgcccgacg acatgggcaa gttgatcgga
     3901 cggcaggggc ggacggtgcg cgcgctgcgt tctctgtttc acgccgtgag cttgaagcag
     3961 ggtcgccgct acatcctaga gatcgtggaa tgagcgagga cgagacgctt gagggcgcag
     4021 aagtcgaagg ggacggcgag gaagagttga tcgccgtcgc gcgcatcgcc aaagtgcgcg
     4081 ggatacgcgg cgaagtggcc gcagacctcc tgaccgactt ccccgaacgc ttcgacggac
     4141 tcgaagagtt gatctcacta aacgcggcgg gcgagcgcgc gacgctctcg attgaaaaga
     4201 gttggctgca cggcgggcgc gtcatactga agtttgccgg gtacgattcg ccggaggcgg
     4261 cgcaggcgtt ggtcgggcgc gaactggccg tgccggaagc cgaggcggtg gaactcgaag
     4321 aggatgagtt ctacgattgg gaattgatcg attgccgcgt cgagacgatt gacaaccgac
     4381 ggatcgggag cgtgcgggaa gtcctgcaca cgggcgcagc cccggtgctc gtcgtgcgcg
     4441 acgagacggc agccgccgca gggcgcgaac acttgatccc gctcgccgag agcatctgcg
     4501 tggagattga cacggaggcg aagttgattc gcgtcgatcc gccggaagga ctgttagagt
     4561 tgtaaatagt gaatcgtaaa tcgtgaatag tgacaaaaca aaagcagttt ctatttacga
     4621 tttacgattt acgattcact aattgtgttg cgcttcgaca tcatcacgat ctttccggac
     4681 tacttccgca cggtgtttga ttacgggatc gtgcggcgcg cgggcgcaag cggtttggtc
     4741 gagatcgagg cgcacgacct gcgcgcatgg gcgtttgata agcaccggat ggttgacgac
     4801 cgcccgttcg gcggcggcga cgggatggtc ttgaagcccg aaccgctctt tgccgcagtg
     4861 gaatctttga cgggcgcgag cgaggcgcgg caactcgcgg gcacggggac gcgcgtggtg
     4921 atgctttcgc cgcagggacg cgtcctgagc cagcaggtcg cggcggaact ggcggaagca
     4981 aaacaactgg tgctcatctg cggtcgttac gaaggcgtgg acgagcgcgt ggtcgaaatg
     5041 ctggcgacgg acgagatctc gattggagat tatgtcctgt cgggcggcga accggcggcg
     5101 gtcgttttag tggatgcgat tgttcgtctg attccc
//
LOCUS       DASWNS010000649         1748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_387627, whole genome shotgun sequence.
ACCESSION   DASWNS010000649 DASWNS010000000
VERSION     DASWNS010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387627"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="VGB76_22460"
     CDS             <1..1746
                     /locus_tag="VGB76_22460"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0101705.1"
                     /translation="LDSRCPGHPEYRWTSGIETTTGPLGQGIATSVGMAIASKWQAAY
                     FNRPGFEMFDYNVYALCGDGCMMEGISHEAASLAAHLKLDNLCWIYDNNKITIEGHTS
                     WAFSEDVATRYIGYGWNVTRVGDANDLETLKRAFNVARRETGRPTLIIVDSHIAWGAP
                     NKQDTHAAHGEPLGEEEIRLTKRNYGWPEEAKFLVPDEVRRHFRDGIGARGKEARDEW
                     FARVENYRKEFPELADQLVRMQKRELPDGWDADIPAFPADAKGLAGRDASGKVLNAIA
                     KNVPWFIGGAADLAPSTKTRLTFEGAGDFTADDYAGRNFHFGIREHEMSAVLNGLSLS
                     KIRPFGSGFMIFSDYSRPAIRLSAIMEIPTIHIFTHDSIGVGEDGPTHQPIEQLASLR
                     AIPGLIVLRPGDANEIAEAWRIVMGLRHEPAVMVLSRQAMPTLDRTKYASAEGVKRGA
                     YVLADNAGEGAQPEIILIGTGTEVSLCVEAYERLVAEGVKARVVSIPSWEIFEHQDEA
                     YKEEVLPPAVRARVAVEQASTFGWRRYVNSNEAIVGMKTFGASAPLKELTKKFGFTVE
                     NVLDTAKREMDRYKS"
BASE COUNT          371 a          546 c          536 g          295 t
ORIGIN      
        1 ctcgacagcc gttgtcccgg ccatccggag tatcgctgga cttccggcat cgaaaccacc
       61 acggggcctc tcgggcaggg catcgccacg agcgtcggca tggctatcgc ctcgaagtgg
      121 caggcggcat acttcaaccg ccccggcttc gagatgttcg actacaacgt ctacgcgctc
      181 tgcggcgacg gctgcatgat ggaaggcatc tcgcacgaag ccgcatcgct cgccgcacac
      241 ctcaaactcg acaacctctg ctggatttac gacaacaaca agatcacgat tgaaggccac
      301 acctcctggg cgttctccga agacgtggcg acgcgctaca tcggctacgg ttggaacgtc
      361 acgcgcgtcg gcgatgccaa cgacctcgaa accttaaaac gcgccttcaa cgtcgcacgc
      421 agagagactg gccgcccgac gctcatcatc gttgacagcc acatcgcttg gggcgcgccc
      481 aacaagcagg acacgcacgc cgcgcacggc gaaccgctcg gcgaagaaga gatcaggctc
      541 accaaacgta actacgggtg gccggaagag gcgaagtttt tagtgcccga tgaagtgcgc
      601 cgccatttca gagacggcat cggcgcacgc ggcaaggaag cgcgcgacga gtggtttgcc
      661 agagtggaaa actaccggaa ggagtttccg gagttggcgg atcagttggt caggatgcag
      721 aaacgcgaac tgccggacgg ctgggacgct gacatccctg cgtttccggc ggacgcgaaa
      781 ggtctggccg ggcgcgacgc ttcgggcaaa gtcttgaacg ccattgcgaa gaacgtgccg
      841 tggttcatcg gcggcgcggc agacctcgcg ccctcgacca agacgcgcct cacctttgaa
      901 ggcgcgggcg atttcacggc tgacgactac gcggggcgca actttcactt cggcatacgc
      961 gagcacgaga tgtccgccgt cctgaacggc ctctcgcttt ccaagattcg ccctttcggt
     1021 tcgggcttca tgatcttctc tgactattcg cgtcccgcga ttcgcctctc ggcgatcatg
     1081 gagatcccta ccatccacat cttcacacac gactccatcg gcgtcggcga agacggcccc
     1141 acgcaccagc ccatcgaaca actcgcttcg ctgcgcgcca tccccggcct cattgtgctt
     1201 cgccccggcg acgccaacga gatcgccgaa gcctggcgca tcgtgatggg actcaggcac
     1261 gaacccgccg tgatggtctt gagccgacag gcgatgccga cgctcgaccg cacgaagtat
     1321 gcgagcgcgg aaggagtgaa gcgcggcgcg tacgttctcg cggacaacgc gggcgaaggc
     1381 gcgcagccgg aaattatctt gatcggcacg ggcacggaag tgtcgctctg cgtcgaggct
     1441 tacgagcgac tcgtggccga aggcgtcaaa gcgcgcgtcg tcagcatacc ttcgtgggaa
     1501 attttcgagc atcaggatga agcgtataaa gaggaggtgc ttccgccggc ggtgcgtgcg
     1561 cgcgtcgccg tggaacaggc gagcactttc ggctggcgtc gctacgtcaa cagcaacgaa
     1621 gccatcgtcg gcatgaaaac tttcggggcg agcgcgccgc ttaaagagtt gaccaaaaag
     1681 ttcggcttca cggtcgagaa cgtgttggac acggcgaaac gcgagatgga tagatacaaa
     1741 agttagcg
//
LOCUS       DASWNS010000650         8100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_465698, whole genome shotgun sequence.
ACCESSION   DASWNS010000650 DASWNS010000000
VERSION     DASWNS010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465698"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VGB76_22465"
     CDS             complement(<1..220)
                     /locus_tag="VGB76_22465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101706.1"
                     /translation="MKWPDSISRLLHEAAAPSRAETGENSVAPHLALGTRGEALAAAY
                     LERAGFMLVAANFKLPVGRNLRGAIVQAE"
     gene            333..1898
                     /locus_tag="VGB76_22470"
     CDS             333..1898
                     /locus_tag="VGB76_22470"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY0101707.1"
                     /translation="MSTSEGNEPDAKASSKKARAFLPLVTIVGRPNVGKSTLFNRLVG
                     ERRSIVGDEPGITRDRIYGEAEWAGRRFKVVDTGGIVPDDDALIPANILKQAETAIQD
                     AVALVWVVDARAGMTPLDEELAHLLRSTGKPVLVAANKTDAVRFEADAGEFYRFGFEG
                     VYPLAAEHGDGVGDLLDALVERFETGAGVEEEFDKEARREIRLAIIGRPNVGKSSLLN
                     RLLGEERVIVSPLAGTTRDAVDTLLEWPLEETDEEGTEDAKVGSSEVESDSANARAAE
                     AGSKRVQRFRLIDTAGIRRKGKTGEMAEKLSVVMARRSLERADVAVVVVDAVEGVTAL
                     DAHIAGYALEAGCSIIIAVNKWDALLNKETGTPFEFERDLRQKMKFLEWAPVVTISAL
                     TGQRVERLLSLAIRANKARTNRITTSQLNDFFERAIDAPGAPSAVAPVKGGRSRLRVQ
                     YVTQVGVRPPTFIVFTAGGAGGKHGLHFSYERYLQNRLREEFDFFATPLRIVERHKRE
                     KSGGGRGKGGRSK"
     gene            1920..2363
                     /locus_tag="VGB76_22475"
     CDS             1920..2363
                     /locus_tag="VGB76_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101708.1"
                     /translation="MHLKAINGALHNFLETYASRYSDYDGYWIFGMLVRELEQFNIDL
                     LNTVDDISDTTPMAAAVRFARANFKDQVEKAGLSLSCLREANLAIIKSSDLGSGFVNG
                     HRSAGFDVSFVAKAVSHRDKTYERKKSVFVAPHDPGVEQRSTRRT"
     gene            2394..3587
                     /locus_tag="VGB76_22480"
     CDS             2394..3587
                     /locus_tag="VGB76_22480"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY0101709.1"
                     /translation="MSKAAINWQVVAREGEARAGLLQTRRALIETPVFMPVGTAGTVK
                     GMRFEALESTELDARIILGNTYHLWLRPGAEVVRACGGLHGFTSWERALLTDSGGFQV
                     WSLGALRKLREEGVEFRSHVDGSARFLSPEVSMEVQAALGAEIVMVFDECAPGEATQE
                     EARASMELTARWAARSRSRFDELQHETGEHGDAGRTPEHHASVNGEQALFGIVQGASH
                     ADLRRESLARTVEIGFDGYAIGGLSVGEAKPVMYDTIEMLAPLMPADRPRYLMGVGTP
                     EDLLEAVARGVDMFDCVLPTRNARTGQAFTSRGKLNIKNARYATDTRPLDDACACSVC
                     RRHERAYLRHLYTTGEMLASILLTHHNLAFFLDTMRRVRQAIRSGDFMRFRREFLERY
                     NSGVE"
     gene            3794..4060
                     /gene="yajC"
                     /locus_tag="VGB76_22485"
     CDS             3794..4060
                     /gene="yajC"
                     /locus_tag="VGB76_22485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY0101710.1"
                     /translation="MIQILPFLLIFAVFYFLIILPQRKRQQQLQATIAALKAGDKIIT
                     TGGIIAKIAEVRDTSLLVRTADKSILEISRAAVAGMHGEEEKKI"
     gene            4115..5836
                     /gene="secD"
                     /locus_tag="VGB76_22490"
     CDS             4115..5836
                     /gene="secD"
                     /locus_tag="VGB76_22490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY0101711.1"
                     /translation="MKKNILRRVIIIVIVTIACLYIVLGPRRRDEQGKLQMPKASDFT
                     AQGLKQTLRDNIRLGLDLKGGTHLVMQVQTDVYLKRLTENTAQAIDTGAKAAGYNVKE
                     VRPDISDGNYRITLTTDDASKLNEMRNELPKKVDLQNWTANVSGNSVTWSLTSAAQRD
                     LSESAVDQAYRIVDSRINAVGVAEPTLQKHGGQNSTQILLQMPGIQDPERVKKLLAGE
                     SRLELMKVVSPPNPAPLKTYTTKEEAIASLGGQVPSNRRVLAYSERDEPTSAGQSATP
                     DPNQAKSWVVVESPAVIDGSELRSADANEGRAGGGSSNYEIDFTLKPSGAEKFGAWTS
                     ANVGTYMAVVLNDEVKSAPFIKGPISDQGQITGNFTEESAKDLALTLRSGALPAPLVY
                     LEERTVGPSLGADSIRAGVTAALGGLVLVVLFMVFYYRGSGLNAVIALLLNMIITVAA
                     LVVFDATLTLPGIAGLILTIGMTVDTNVLIFERIREELETGKTVASAVEQGFSRAFVT
                     VIDTHVTTIVSSLFLFVFGTGPIRGFAVTLILGLLANLFSAVYVSRTIFIWLLNRKGR
                     RAETLSI"
     gene            5933..6994
                     /gene="secF"
                     /locus_tag="VGB76_22495"
     CDS             5933..6994
                     /gene="secF"
                     /locus_tag="VGB76_22495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY0101712.1"
                     /translation="MIRLLHNVKIDWLAQRRFWLVASALLMLAGLGSAVARQAAGGGT
                     SAFNLGVDFKGGTVMTARFNQRPSDDAIRTALEQKGVRNAIVQSTERPNEVLIRVPEQ
                     GSGEQAQSQAQVDVGRALAGEALTALGPEVGATIERTEAVGPVAGRALRNQAIAVTLL
                     ALVGVLLYIGSRFEWTYGAAAVITVFHDVLVTLGIFSIMQWEIDLTVVAALLTLVGFS
                     VNDTIVTFDRIRENLRLQRRASLYDLTNEAINQTLSRTVITAGLVFFSVLALVVFGGD
                     VLRGFSLALFIGIIVGTYSTIAIATPLMVWFQQRLEPAKRTTATREGGAQASGSAPLS
                     SRSAKAKAAARGSQRTARS"
     gene            7415..>8100
                     /locus_tag="VGB76_22500"
     CDS             7415..>8100
                     /locus_tag="VGB76_22500"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0101713.1"
                     /translation="MFDSLVESGSHKDDLARKGSFFLGTFAIYAVILLALGVLSVYLY
                     NESLPSDNLELMTMVAPVPVQNQPEQQPEKQAESKPAEATPDRQVATRTDFVASNLDP
                     TKVPERVSAVASKVPPVPPGGAVRGTSNTDPVGGNTGPIGPVGPGTGGVGTGGSGAPV
                     VVNTDDPEPAPPPKPTPTPAPKPRAPISGGVLNGKAISKPQPAYPAIAKAARASGTVT
                     VQVTVDEQGNV"
BASE COUNT         1562 a         2418 c         2565 g         1555 t
ORIGIN      
        1 tctcggcctg gacgattgcg ccacgcaaat ttctcccgac cggcaacttg aaattggccg
       61 cgacgagcat gaagcccgcg cgttcgagat aagctgcggc cagagcttcg ccgcgtgtgc
      121 cgagcgcgag atgcggagcg acggagttct cgcccgtttc tgcccgcgac ggcgccgctg
      181 cttcatgaag taagcgcgaa atgctgtcag gccatttcat ggtgcgtcta tcccgtgagg
      241 cgtgggagcg gtcgttggtt tgccggggtt tgccggagcg cgcggggcgc gtctttgacg
      301 tgacgcggaa gtatagcgca tcatctgtag ctatgagtac aagtgaaggt aatgaaccgg
      361 acgcgaaggc gtcttcaaag aaagcgcgag cctttctgcc gctcgtcacc atcgtcgggc
      421 gaccgaacgt ggggaagtca acgctcttca atcgtctcgt cggcgagcgt cgttccatcg
      481 tcggcgacga gccgggcatc acgcgcgacc gcatctacgg cgaagcggaa tgggcggggc
      541 gtcgcttcaa agtggtggac acgggcggca tcgtgccgga cgacgatgct ttgatccccg
      601 cgaacatttt gaagcaggcg gagacggcga ttcaggatgc cgtcgcactc gtctgggtgg
      661 tggacgcgcg cgccgggatg acgccgctcg acgaagaact cgcgcacctc ttgcgctcta
      721 cgggcaagcc cgtgctcgtc gcggcgaaca agacggacgc cgtgcgtttc gaggccgacg
      781 cgggcgagtt ttatcgcttc ggcttcgagg gcgtctaccc gctcgcggcg gagcacggcg
      841 acggcgtggg cgatctgctc gatgcgctcg tcgagcgttt cgagacgggc gcgggcgtcg
      901 aagaggagtt tgataaggaa gcgcggcgcg agatacgcct cgcgatcatc gggcgtccga
      961 acgtcggcaa gtcgtctctg ttgaaccgcc tgctcggcga ggagcgcgtc atcgtctcgc
     1021 cgctcgcggg cacgacgcgc gacgcggttg acaccctgct cgaatggccg ctcgaagaga
     1081 cggatgaaga gggcacggag gatgcgaaag ttggaagttc ggaagtcgaa tccgacagtg
     1141 cgaatgcgag agcggcggag gcggggtcga agcgcgtgca gcgttttcgt ctgatcgaca
     1201 cggcaggcat caggcgcaag ggcaagacgg gggagatggc cgagaagttg tcggtcgtga
     1261 tggcgcggcg cagtctggag cgcgcggacg tggcggtcgt cgtcgtggat gccgtcgaag
     1321 gcgtgaccgc gctcgacgcg cacatcgcgg gctacgcgct cgaagccgga tgctccatca
     1381 tcatcgccgt gaacaagtgg gacgcgctcc tgaacaaaga gacgggcacg cctttcgagt
     1441 ttgagcgcga cctgcgccag aagatgaaat ttttggaatg ggcacccgtc gtcacgatct
     1501 cggctttgac cgggcagcgc gtcgaacgcc tcctgtcgct cgccatccgc gccaacaagg
     1561 cgcgcaccaa tcgcatcacc acttcgcaac tcaacgactt cttcgagcgc gcgattgacg
     1621 cgccgggcgc gccttcggcg gtcgcgccgg tcaagggcgg gcgttcacgc ctgcgcgtgc
     1681 aatacgtcac gcaggtcggc gtgcgcccgc ccaccttcat cgtcttcacg gcgggcggcg
     1741 cgggcggcaa gcacggactc cacttctcct acgagcgtta cctccaaaac cgcctccgcg
     1801 aagagttcga cttcttcgcc acgcccctgc gcatcgtcga acgccacaag cgcgagaaga
     1861 gcggcggcgg gcgcggcaaa ggcggacgct cgaaatagcg tcaactacct gacaagattg
     1921 tgcatctcaa agctatcaac ggagccttgc ataatttcct cgaaacctac gcgagccgtt
     1981 actcggatta cgatggttac tggattttcg gcatgctagt gcgcgagttg gagcagttta
     2041 atatcgatct cttaaataca gttgatgata tatccgacac tacgccgatg gctgctgccg
     2101 tgcgatttgc ccgcgccaat ttcaaggatc aggtggagaa agctggtctt agcctgtcgt
     2161 gtctccggga agccaacctc gcgatcatta agtcttcaga cttaggaagc ggctttgtta
     2221 atggtcatag gtctgccggc tttgatgtaa gttttgttgc taaagcggtg tcacacaggg
     2281 ataagacgta tgagcgtaag aaatcagtat ttgttgcgcc tcatgatccc ggcgtggagc
     2341 agcgaagcac acggcgcact taaatataga gatgagtgca ggcggaaacg gcgatgagta
     2401 aagcggcaat taactggcag gtcgtggcgc gggaggggga ggcgcgtgcg gggttgttgc
     2461 agacgcgtcg tgcgctgatt gagacgcccg tgtttatgcc cgtcgggacg gcggggacgg
     2521 tgaaggggat gcgcttcgag gctttggaat cgacggaact cgatgcgcgg atcattctgg
     2581 ggaatacata tcatttgtgg ttgcgacccg gagcggaggt ggttcgggca tgcggcggct
     2641 tgcacggctt tacgagttgg gagcgcgcgc tgttgaccga ttcgggcggc tttcaggtgt
     2701 ggagtctcgg cgcgttgcgc aagttgcggg aagagggcgt ggagtttcgt tcgcatgttg
     2761 acgggagcgc gcgttttctg tcgccggaag tctcgatgga agtgcaggcc gcgctcggcg
     2821 cggagatcgt gatggtcttt gacgagtgcg cgccgggcga agcgacgcag gaggaggcgc
     2881 gcgcctcgat ggaactcacg gcgcgctggg cggcgcgttc gcgcagccgt tttgacgagt
     2941 tgcagcacga aacaggggag cacggggacg cggggcgcac gcccgaacat cacgcgtcgg
     3001 tcaacggcga acaggctctg ttcggcatcg tgcagggcgc gtcccacgca gacctcaggc
     3061 gcgagagtct cgcacggacg gtcgaaatcg ggttcgacgg ctacgccatc ggcggcttga
     3121 gcgtgggcga agcgaagccc gtgatgtatg acacgattga gatgctcgcg ccgctgatgc
     3181 ccgccgacag gccgcgttat ttgatgggcg tcggcacgcc ggaagacctg ctcgaagccg
     3241 tcgcgcgcgg cgtggacatg ttcgattgcg tgctgccgac cagaaacgcg cgcaccgggc
     3301 aggcgttcac ttcgcgcggc aaactcaaca tcaagaacgc gcgctacgcg accgacacgc
     3361 gcccgctcga cgacgcctgc gcctgctccg tctgccgccg ccacgagcgc gcctacctgc
     3421 gccatctcta cacgacgggc gagatgctgg cgagcatttt actgacgcat cacaatctcg
     3481 cgttcttcct tgacacgatg cgacgcgtca ggcaagctat ccggtctggt gatttcatgc
     3541 gatttcggcg agagttctta gagcgttaca actcgggtgt cgagtgagtt ttcccgtccg
     3601 tgctcgcaag cgtcgggacg cttcacacga gacatcgcgg gtcgcgccgc gctccagaag
     3661 gacgttgcac gcccgttttc cggcgcgcca caaacgtcgc cttgcacaga tttacaactg
     3721 ctcaaaattt actaatagct gaaaacttaa gaccatgaca tttgccttca tactgcaatc
     3781 ggcgatcggg ccgttgattc agattctccc gttcctgctc atctttgcgg tcttctattt
     3841 tctgatcatt ctcccgcaac gcaagcggca gcagcagctt caagccacca tcgccgcttt
     3901 gaaggcgggc gacaagatca ttacgacggg cggcatcatc gccaagatcg ccgaggtgcg
     3961 cgacacgtcg ctcctcgtca gaacggcgga taagtccatc ctggaaattt cccgcgcagc
     4021 ggttgcaggc atgcacggcg aggaagagaa gaaaatctag aatttgctcc ggcgcgccct
     4081 ttcgagtgca aagctgcgcg cccctgattt tgttatgaaa aagaatatcc tacgacgcgt
     4141 cattatcatc gtcatcgtga ccatcgcctg tctctacatc gtgctcggtc cgcgccgccg
     4201 cgacgagcag ggcaagttgc agatgccgaa ggcgagcgac ttcaccgcac agggactcaa
     4261 gcagaccttg cgcgacaaca tccggctcgg cctcgacctc aaaggcggca cccacctcgt
     4321 catgcaggtg cagacagacg tgtacctgaa gcgtctgacg gagaacaccg cgcaggcgat
     4381 tgacaccggg gcaaaggccg ccgggtacaa cgtcaaagaa gtgcgcccgg acatctcgga
     4441 cggcaactac cggatcacac tgacgaccga cgacgcctcg aagttgaacg agatgcgcaa
     4501 cgagttgccg aagaaagtcg atttgcagaa ttggacggcg aacgtctccg gcaattccgt
     4561 cacgtggtct ttgacttcgg cggcgcagcg cgatttgagc gagtcggcgg ttgatcaggc
     4621 gtatcgcatc gtcgatagcc gcatcaacgc agtcggcgtg gccgaaccga ctttgcagaa
     4681 gcacggcggg caaaactcca cccagattct cttgcagatg ccgggcatcc aagacccgga
     4741 gcgcgtgaag aaactgctgg cgggcgagtc gcgcttggaa ttgatgaagg tcgtcagccc
     4801 tccgaatcct gcgccgctca aaacttatac gacgaaggaa gaggccatcg cctcgctcgg
     4861 cgggcaggtg ccgtcgaacc gccgcgtgct ggcctatagc gaacgcgacg agccgacctc
     4921 tgccgggcag agcgcgacgc ccgacccgaa tcaggcgaag agttgggtcg tggtcgagtc
     4981 gcccgcagtg attgacggca gcgagttgcg cagcgcagat gccaacgagg ggcgcgccgg
     5041 aggcggcagt tccaattacg aaatcgactt cacgctcaag ccttcgggcg cggagaagtt
     5101 cggcgcgtgg acgagcgcga acgtcggaac ctatatggcc gtcgtcttga acgacgaagt
     5161 caaatccgcg cccttcatca aagggccgat tagcgatcag ggacagatca ccggcaactt
     5221 cacggaggaa tcagctaaag acttagcctt gaccttgcgt tcgggcgcgc tccccgcgcc
     5281 gctcgtctat ctggaggagc gcaccgtcgg gccaagcctc ggcgcagact ccatccgcgc
     5341 aggcgtcacc gccgcgctcg gcgggttggt gctcgtcgtc ctctttatgg tcttctacta
     5401 tcgcggttcg ggcttgaacg ccgtcatcgc gctactcttg aacatgatca tcacggtggc
     5461 cgcgctcgtc gtcttcgacg cgacgctgac gctgcccggc atcgccgggt tgatcttgac
     5521 tatcggcatg accgtggaca cgaacgtgct catcttcgag cgcatccgcg aggaattaga
     5581 gacgggcaag acggtcgctt cggcggtcga gcaaggcttc tcgcgcgctt tcgtcaccgt
     5641 catcgacacg cacgtcacga cgatcgtgtc gtcgctcttt ttgttcgttt tcggaacggg
     5701 gccgattcgc ggtttcgccg tcacgcttat tctcggtttg ctcgcaaacc tgttctcggc
     5761 agtttacgtc tcgcgcacca tcttcatctg gctcttgaac cgcaagggaa gacgcgccga
     5821 aacgctcagt atttaacagc agtgacgggt gacaggcgac gagtgacaag ttgaaaacag
     5881 gcttgtcttt aactcgtcac ctgtcgcctg tcacctgtca cttttaaaaa tcatgattcg
     5941 acttcttcat aacgtaaaaa tcgattggct cgcacagaga cgcttctggc tcgtcgcttc
     6001 ggcgctcctg atgctggcag gcttgggttc ggcggttgcg cgacaggcag cgggcggggg
     6061 cacaagcgcc ttcaacctcg gagttgactt caagggcggc acggtgatga cggcgcgctt
     6121 caatcaacgc ccttcggacg atgcgattcg cactgcgctc gaacagaagg gcgtgcgcaa
     6181 cgccatcgtg cagtcaaccg agaggccgaa cgaagttttg atccgcgtgc cggagcaggg
     6241 cagcggcgag caggcgcaga gtcaggcgca ggtggatgtc gggcgcgcgc tcgcgggcga
     6301 agcgctcacc gccttgggtc ccgaagtcgg cgcgacgatt gagcggacgg aagccgtcgg
     6361 ccccgtcgcc ggacgcgccc tgcgtaatca ggccatcgcc gtcacgctgc tcgcgctcgt
     6421 cggcgtcctg ctctacatcg gctcacgttt cgaatggact tacggcgcgg cggcggttat
     6481 cacggtcttc cacgatgtgc tcgtcacgct cggcatcttc tccatcatgc aatgggagat
     6541 cgacctgacg gtggtggccg cgttgctcac gctcgtcggc ttctccgtca acgacaccat
     6601 cgtcaccttc gaccgcatcc gcgaaaacct gcgcctgcaa cgtcgcgcct cgctctacga
     6661 tctcacgaat gaggccatca accagacgct ctcgcgcacg gtcattaccg ccggactcgt
     6721 cttcttctcc gtgctcgcgc tcgtcgtttt cggcggcgac gtgctgcgcg gcttctcgct
     6781 cgcgctcttt atcggcatca tcgtcgggac ctactcgacc atcgccatcg cgaccccgct
     6841 catggtctgg ttccagcagc ggctggaacc cgcgaaacgc acgaccgcga cgagagaagg
     6901 cggcgcacaa gcctccggaa gcgcgcctct cagctcgcgc agcgcgaagg cgaaagccgc
     6961 cgcgcgcggc tcgcaacgca cggcgcgttc atgagccgca cggcgcgttc tgtcaggtta
     7021 agagcgcgtt aactaaagat tttacgcttg acttgaacag gcatcgcgac atagactgag
     7081 cgcgttgctg gcccaattaa atcgctttaa caagaacgct cccgtctgca cgcattcgtg
     7141 tcgttgacgg tgacgcgtga ttgtaggttt ttgccggaag tggtcggtag gccgatctgt
     7201 ttccctgcgc agcgtgtgtg aatattgacg gttgcgcgct cttcgcgccc gcccgccgct
     7261 gctacccccg ctataatttt aagtttcaat ttcattttac gggtgagttt cgccgtctcg
     7321 gacgttttgg gaacataacg gttcccaaag gcatctaatg agttgatatt tcacacccct
     7381 gatccattgg caccggaagt gggaggataa aaagatgttc gatagtttag tggagtctgg
     7441 ctcgcacaag gacgatctgg cgcgcaaagg ctcgtttttt ctcgggacgt ttgccatcta
     7501 cgcagtgatc ctgctggctc tcggcgtcct cagcgtctat ctatataacg agagtttgcc
     7561 gagcgacaat cttgagttga tgacgatggt ggctcccgtc cccgtccaga atcagccgga
     7621 acaacagccg gaaaaacagg cggaatcgaa acccgccgaa gctacgcccg acaggcaggt
     7681 ggcgacccgc accgatttcg tcgccagcaa cttagacccg acgaaagtgc cggagagagt
     7741 ctctgccgtg gcgagcaaag ttccgccggt tccgccgggg ggcgccgtgc gtggcacctc
     7801 gaacactgat ccggttggcg gtaacaccgg ccctatcggc cccgtcggcc ccggaacggg
     7861 cggtgtcggg acgggtggta gcggcgctcc ggtcgtggtc aacacagacg atcctgagcc
     7921 tgcgcctccg ccgaagccga cgccgacgcc cgcgcccaaa ccgcgtgcgc cgatctcggg
     7981 cggcgtcttg aacggcaagg ccatcagcaa gccgcaaccc gcgtatccgg cgattgccaa
     8041 ggccgcacgc gcctccggca cggtcacggt gcaagtgacc gttgacgaac agggcaatgt
//
LOCUS       DASWNS010000651         5069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_387707, whole genome shotgun sequence.
ACCESSION   DASWNS010000651 DASWNS010000000
VERSION     DASWNS010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387707"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(480..2522)
                     /locus_tag="VGB76_22505"
     CDS             complement(480..2522)
                     /locus_tag="VGB76_22505"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEY0101714.1"
                     /translation="MKALKLFALAVFASLLVTSLALTTAVRSQSGPTEAPVNFDNVTN
                     GFTTQAQFDLDRAAFEQREEKAGGLGPVYNAQSCAECHQNSVTGGSSQIFELRAGHSG
                     TDGTFVPAPGGSLIQSRSIDTQLQERVPDGARIAFVNHLNQISVMSVNGGENGAVGNI
                     PAVGSYPSFSPDGTKIAFRSNSQIWVMNNDGTNKQQLTTAGQNFTPAWSPDGTKIAFT
                     SNRTGPFQVFMMNQDGTSQTNISNSAANDGAPAWSPDGTKIAFTRSPQASNPTGKIWM
                     MSPNGLGQTQVTTGAGNDERPSYSPDGTMIAFSSNRDGNYEIYKILAVGGIVTRLTNN
                     PASDRNPAWAPDDSAIAFGSDRNAGMKIWAIAPDGANPSQLSGNNDGGNVPAYSRDQG
                     ETVRTFRASPHVLGLGFVEAIDDSTLLAIRDGQPAAMRGTAIFVPLLEAPGQTRLGRF
                     GWKNSVASLLSFSAGAYLFEMGITSPLLPNEQTSLGRSVAQVDTVPDPEDTGGDQGFG
                     EDVEAFTRFMRSTMAPSRSLQLSLNDATDPGSALFDSLSCSVCHVRSITTAPAGTVIN
                     GGTFAVPPALGDKVIHPFSDFLLHDIGTGDGIVETNGELTRNKMRTAPLWGLRTRDRF
                     MHDGGSASPPSNNGAQSFALDEAILRHAGQATASRTAYQALTPLQKAQLIRFLKSL"
     gene            3516..>5069
                     /locus_tag="VGB76_22510"
     CDS             3516..>5069
                     /locus_tag="VGB76_22510"
                     /inference="COORDINATES: protein motif:HMM:NF013604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY0101715.1"
                     /translation="MSKLRRTPARKVAAVILVGCISMALVLPSSLAQKADVSPQKKEA
                     AQSPSRQVEVPTAVLTDAVNNKRVDKGAGVAEVGKPLFAQGGKESLERAKLISLEILS
                     QQVQTRSVGAINDLKAEKVEIDDLQMAHTRVRQTVEGIPVWAGEAIVHLKSDGSLFAI
                     TDDLKEGVSVDTKPSISADEAVRIANNLYTGTAVETEAAKVDLWVYRGEDRDHLTYRV
                     EIPRLDGSEGTSIPVDFIDAQTGERVFGYDNLQTGTGASLYSGTVTINTSSSGQNFFM
                     EDLTRRMGTYNNNGTGNADTDDPGTQSPFVDGDDNWNATIQRAGVDAHYGAAKTFDYY
                     LNVHGRNGIDGSGGPRTTAASANGAIGLITSRVHFGNAYKGAFWDPNRKTMNYGDGDG
                     VLLSPLVALDICGHEMTHGVTQHTANLVYAGESGALNESMSDVFGTMIERFARPATWN
                     WKAGEDVYTPGTPGDAIRYMDNPRLDGQSPDHYSQRLHPGFCTPDPRNDNCGVHYNSG
                     IANHAFYLIA"
BASE COUNT         1025 a         1402 c         1574 g         1068 t
ORIGIN      
        1 acctcctccg cataccccgt ctacagtgcc agttaagaaa cattcgggca gatgagctgt
       61 atcgcaattg gcgccaccac aaattgcggt cggacgcgcc ttttcaaaaa gggcgtgaaa
      121 cctccctcac gactctaaat tgcacacctc gtcagcctac atcttatggt cgagatctct
      181 ttcgagggag gtttcacttt atcaagtcgg ataagagtca ttatgtgcaa gtaaccttgc
      241 caaaaagtct aatgggatct cattagttta gcttcacatc acggccttaa actttatcct
      301 gtggctgggc gggacttttc ggcaaagctc aggcaacccg cgatgtttaa cgcttcctca
      361 gggccttgaa actcaccacg taaatcaatg ccggggacgg gttcggctcc cgcccccggc
      421 aaactactac gcgcgcgccg cgaagcacac cggcctcgcg tcggtcactg tcgtcctcct
      481 cacaacgatt tcaggaacct gatgagttgc gccttctgaa gcggcgtcag cgcctggtag
      541 gcggtgcggc tggccgtagc ctgcccggcg tggcgcagta tcgcctcgtc gagggcgaag
      601 gactgcgcgc cgttgttcga gggcgggctc gcggagccgc cgtcgtgcat aaagcggtcg
      661 cgcgtcctca gtccccacag cggcgcggtc cgcatcttgt tcctggttaa ctccccgttg
      721 gtctccacta tcccgtcgcc cgtcccgatg tcgtgcagca ggaaatcgct gaaggggtgg
      781 atcaccttgt cgccgagcgc ggggggcacg gcgaacgtcc cgccattaat gaccgttccc
      841 gcgggcgccg tcgttatcga gcggacgtgg cagaccgagc aactgagcga gtcgaagagg
      901 gccgagccgg ggtcggtcgc gtcgttcaag gagagctgga ggctgcggga cggggccatg
      961 gtcgagcgca tgaagcgcgt gaacgcctcc acgtcctcgc cgaacccctg gtcgcctccg
     1021 gtgtcttcgg ggtccggcac cgtgtcgact tgcgcgacgc tgcgacccag cgacgtctgc
     1081 tcgttcggca ggagcgggct ggtgatgccc atctcgaata agtaggcccc ggcggagaag
     1141 ctgagcaggc tggcgaccga gttcttccag ccgaagcggc cgaggcgcgt ctgccccggc
     1201 gcttcgagca gcgggacgaa gatggcggtg ccgcgcatgg ccgccggctg gccgtcgcgt
     1261 atggcgagca gggtcgagtc gtctatggcc tcgacgaagc cgaggcccag gacgtggggc
     1321 gaggcgcgga aggttcgcac cgtctcaccc tgatcccttg agtaggcggg aacgttgccg
     1381 ccgtcattgt tcccgctgag ctgggacggg ttagcgccgt cgggcgcgat ggcccagatt
     1441 ttcatgccgg cgttgcggtc gctgccgaag gcgatggccg agtcgtcggg cgcccaggcc
     1501 gggttgcggt ctgaggccgg gttgttcgtc agccgggtca cgatgccacc gaccgcaagt
     1561 atcttgtaga tttcgtagtt gccgtcgcgg ttcgagctga aagcgatcat cgtcccgtcg
     1621 ggtgagtagg acggccgctc gtcgttgccc gcgccggtcg tgacctgcgt ctgccccaac
     1681 ccgtttgggc tcatcatcca aatcttcccc gtagggttgc tggcctgcgg cgagcgcgtg
     1741 aaggcgatct tcgtgccgtc gggtgaccaa gcaggtgcac cgtcattggc ggctgagttg
     1801 gagatgttcg tctggctggt gccgtcctga ttcatcatga atacctggaa gggccccgtg
     1861 cggttactgg tgaaggcgat cttcgtcccg tcgggcgacc aggcgggcgt gaagttttgg
     1921 ccggccgtgg tgagttgttg cttgttcgtc ccgtcgttgt tcatcaccca gatttgggag
     1981 ttgctgcgga aggcgatctt cgtcccgtcc ggtgagaagg atgggtagct ccccacggcg
     2041 gggatgttgc ccacggcccc attctcgccg ccgttaacgc tcatgacgct gatctgattg
     2101 aggtggttca cgaaggcgat acgtgccccg tcggggacgc gctcctgaag ttgcgtgtcg
     2161 atgctgcgcg actgaatgag cgaaccgccg ggcgcgggaa cgaacgtccc gtcagtcccg
     2221 ctgtgcccgg ccctcagctc gaagatctgg ctggagccgc cggtgacgct gttctggtgg
     2281 cactcggcgc acgactgcgc gttgtagacc ggcccgaggc cgccggcttt ctcctcgcgc
     2341 tgctcgaagg cggcgcggtc caggtcgaat tgagcctggg tggtgaagcc gttggtcacg
     2401 ttgtcgaagt taacgggcgc ctcggtcggg ccggactggc tcctgaccgc cgtcgtgagt
     2461 gccagcgaag tgacaagcag tgaggcgaag accgcgaggg cgaacagctt gagagctttc
     2521 atgttttctc ctgagataga agccgctttc ggctttaccg gaagcggtgg ggcgccggca
     2581 gcggcccgcg gacgtgaagc ttgaaaaccg ggttgcgacc ccggaacggc aaaaagttgc
     2641 ctgaattcaa tgtgggccga gacgtggcca cgccgacgaa gaggctcccc gcgtgccgcc
     2701 tgatgagaag cggcgagagc gagtggcttc gaaagaagag cgtgcggctg tgcttcgtgc
     2761 cgccgctgcc ggtgaatagg tgtacgtgag gttaacggaa gaggagcagg ctcctcccgt
     2821 taacagagaa acgagcgcgc tgtgaccact cggctcaacg tctgggtgaa gttagggccg
     2881 gtgaggttaa ggtcgcgcat cctactgatc gggtcaaaga ttgggtgtag cgcgggtaaa
     2941 tttactcgca tcggatattg gcgtcaatag aattatgcta aagcgccaat acggtcattg
     3001 tgtcttgtta atttgtgcga cataaaagaa cggccgctcc tttgagaggc tgttcgcgtc
     3061 ctataatata tattacgtcg gattcacccg cacttttgat gacgctggag cagacctctc
     3121 gcggtttact gaaccagcag ctgtattacg ttgaatggag tacccgcgct actctgcgcc
     3181 gcttacggct atgatttagg gaaaatcatt gacagtaact gaagtcttca atatattggc
     3241 ggcccattct caacgggttg cccactaacc tctgtgtgct acgggcttcc cactttgtat
     3301 tttgtacctg ctgtcctcta tgactcacac cattccaaat catggcattt cagccatagt
     3361 gacagcaaca ggcgcgtcga actcctcgct gtcagcctga tttacccgct ggtgctccgg
     3421 ctgatcaact ttttgaaacc ccggccacgt gacttaactt gagccgtcaa gtttaagctg
     3481 cctgttagcc acccgcatct cccaagaggc tcaccatgtc gaaattaaga agaacccctg
     3541 cgcgaaaggt cgccgctgtt attttggtcg gctgcatctc gatggcactt gtattgccct
     3601 cgtcattggc gcaaaaagcg gatgtcagtc cgcaaaagaa agaagcagcc caaagcccaa
     3661 gtcgacaggt agaagtcccg accgccgttc tcacagacgc ggttaacaat aaacgggtgg
     3721 acaagggagc gggtgtggcc gaagtgggca aacccctttt cgcgcagggc gggaaagaaa
     3781 gcctcgaaag ggcaaagctg atcagtctgg aaattctgag ccagcaggtg cagacgcgca
     3841 gcgtcggagc tattaatgac ctgaaggctg aaaaggttga aatagacgat ttgcaaatgg
     3901 cgcatacgcg cgtgcggcaa acggtggaag gcattccggt ttgggcaggg gaggcaatcg
     3961 ttcacttgaa atcggacggt tcgctcttcg cgattaccga tgaccttaag gaaggggtca
     4021 gcgtagatac caagccgagc atttcggcgg atgaggctgt gcgtattgcc aacaacttgt
     4081 acaccggcac agcggtagaa accgaagcgg cgaaagtgga cttgtgggtc tatcgcggcg
     4141 aggaccgcga tcatctgacc tatcgagtcg aaataccgag gcttgatggt tccgagggca
     4201 cctccatacc ggtcgatttc atcgacgcgc aaacaggaga acgggtgttc ggttatgaca
     4261 atttacagac cggcacgggt gcgtcgctct acagcggaac ggtgacgatc aacactagta
     4321 gctcgggaca gaattttttc atggaagact taacccgccg gatgggtacc tacaataata
     4381 atggcaccgg taatgctgat accgacgatc caggtactca gtcaccgttc gtcgatggcg
     4441 atgacaattg gaatgccacg atccaaaggg ccggcgttga cgcgcattat ggagcggcga
     4501 agacgtttga ctactatctt aatgttcacg gccggaacgg cattgatggc tcaggtggtc
     4561 ccagaacgac agccgccagc gcgaacggtg cgataggctt gattacctcc agagttcatt
     4621 tcggcaatgc atacaagggt gccttttggg accccaacag aaaaacgatg aactatggag
     4681 atggcgatgg ggttctgttg tcgccgctgg tagcccttga tatttgcggc cacgaaatga
     4741 cacacggcgt tacgcagcat acggcaaact tggtttatgc gggtgaatcc ggcgcgctga
     4801 acgagtcgat gtcagatgtt tttggaacaa tgattgaaag gttcgcgcgc ccggcaacct
     4861 ggaattggaa agcaggtgaa gacgtctata cgccaggaac tccgggcgac gccatacgtt
     4921 atatggacaa tccgcgcctt gatggacagt ctccagacca ctatagccag cggctccatc
     4981 cgggattttg cacccctgac cctaggaatg acaattgcgg cgtgcactat aattcaggta
     5041 ttgccaacca tgctttttat ttgattgcg
//
LOCUS       DASWNS010000652        20142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_207215, whole genome shotgun sequence.
ACCESSION   DASWNS010000652 DASWNS010000000
VERSION     DASWNS010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20142
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207215"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1438)
                     /locus_tag="VGB76_22515"
     CDS             complement(8..1438)
                     /locus_tag="VGB76_22515"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0101716.1"
                     /translation="MSASFTLPHLPIEQFTLANGLRVVLSEDRSVPVVSVCVYYDVGS
                     RNEREGRTGFAHLFEHMMFQGSENVPKAAHFQYIFNSGGTMNGTTSSERTNYYETLPA
                     NQLPLALWLESDRMRSLKVTQENLDNQRNAVQEEKRLNYDNRPYSNAFLRLNELIYRN
                     PANAHSTIGSMQDLDDATIEDIKEFFRIYYAPNNAVLIICGDFDSREARALVEKYFAT
                     IPSQPAPPPVDVSEPLEVAAPFEKYPDPFAQLPALMLGWKAPARRTPDAYALTLASEL
                     LLEGESSRLYQKLVKGDESVVAVQGGVGERRGPSSLYLFAIPKPGRTTEEIRRTINEE
                     IARLATEGPSAEEMEKLRNTLLNDSVRGRQTTMYRAQRLAEYTLYDGDPHLVNTEIEL
                     YLSVTPEQIKDVVTRYLHTDNHALIEVIPSPEGTERAEPAADASAVATEEKDQPGAPP
                     PQVPPPPAGQPAPATETPLVEVTSDK"
     gene            complement(1448..1933)
                     /locus_tag="VGB76_22520"
     CDS             complement(1448..1933)
                     /locus_tag="VGB76_22520"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0101717.1"
                     /translation="MKRVFVLFIFCAFASVCAADALAQSRRVRRSEPPQEERAGKSET
                     KEEAKDGAQDQPQDGAQADDEPQTVRGVTKRAVIKSKPNPGYPVEARGYGVRGVVKLR
                     IILGSDGKVRDKIKVIEGLPHGVTEEAIKAARRIKFEPAEKEGRPVSQYVIVIYHFNL
                     Y"
     gene            complement(2003..2464)
                     /locus_tag="VGB76_22525"
     CDS             complement(2003..2464)
                     /locus_tag="VGB76_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101718.1"
                     /translation="MSSPYDPNNPHSPDDPSPVNQSDMIDDLMLREDEDETGHGADRE
                     RMSSSVRDIASRIPESLSSIGRDISRTIERAISAKDDYVVAVKVSHEAQEKLENLVQA
                     GVFRSRAEAAAFLIDEGIKSQTPLFERVQQKLHEIERLRAELRGMINTPQS"
     gene            2910..4679
                     /locus_tag="VGB76_22530"
     CDS             2910..4679
                     /locus_tag="VGB76_22530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0101719.1"
                     /translation="MKQKISSALVALLLVALSLPTSVLAQQPAPERRGTAPGQSMPPN
                     VGAPAQRGTGRQRQRVPASSGATAAIEEDFAEALSIVQENYVDGVKIDYNAAFKSSII
                     GMLRSLDPHSNYYDSKEFEELQADWRSEYYGIGATIGDRTIAGQTDTYILATFPGAPA
                     TRAGLRFADRIVEVNGEAIKGKNSSDVRDRLRGPKGSTVKVTVERAATKTLETVEITR
                     DAVGQPTVPDSYMIKPSVGYVDMTRGFNRSTAEEFSAALEDLHKQGMTSLVLDLRGNP
                     GGLLDQAVKVAERFLQRGQVILSQKGRVPGSERTYESRNSSPDSVPLVVLVNRNSASA
                     SEIVAGALQDHDRALIIGETSFGKGLVQSIIPLEYGTAVTLTSSKYYTPSGRLIQRDY
                     TNEGLYDYYTRGGVGSLDADNKAAEPAKPSGPESLTDTGRKVYGGGGIAPDEPVKPRI
                     IEPAQQRLTDPIFAFVRELVNGRIKGFENYKVARAIDYNHDIKAEDYPVTDNLFKAFK
                     TFVANDETLKVKEAQLDKSREFITRQMRYDIATAAYGTVTASQVLVTDDPQVAKAIDV
                     LPRARDLSTAAMRGRNPERKTFE"
     gene            complement(4788..5459)
                     /locus_tag="VGB76_22535"
     CDS             complement(4788..5459)
                     /locus_tag="VGB76_22535"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEY0101720.1"
                     /translation="MRRFFRNNGLSLVLFVLFFFTLIVGQSVVGQREYNNDQQAHGQP
                     AAGYAEYLWTPHFLEATMENWESEFLQMFIFIVLTIFLYQKGSAESKDPDEEEAVDRD
                     PRNSRNKKDAPWPVRRGGFVLKLYENSLSLTFFLLFLISFWLHAVGGAGEYNQEQIAH
                     GNAGEQVSALAYMGTSRFWFESLQNWQSEFLAIGMMVLLTVWLRQKGSPESKPVDAPH
                     DRTGE"
     gene            complement(5512..6171)
                     /locus_tag="VGB76_22540"
     CDS             complement(5512..6171)
                     /locus_tag="VGB76_22540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY0101721.1"
                     /translation="MPAKNEDLHGFVPDKAEVALLLIDVINDLEFSSGGKLLEHALPM
                     AEKLAALKQRAKEAKIPVIYVNDNFGKWQSDFKSVLKHCLEEDVRGRPVAELLQPQED
                     DYFVLKPKHSGFFSTTLDTLLDYLQVKSLILTGLTGDICVLFTANDAYMRDFHLLIPA
                     DCVASSDADENRHALEHMQRVLKADTRPSNEINFDELKQRAGHDEPKPQPQTRQFAKQ
                     D"
     gene            complement(6352..6798)
                     /locus_tag="VGB76_22545"
     CDS             complement(6352..6798)
                     /locus_tag="VGB76_22545"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEY0101722.1"
                     /translation="MKINYAAVAVSAIGYWVLGGIWYSPLLFGGKFIEVMRWSPEQIA
                     RVQAEGAGAQLALAFVGSLVAAYVLAHIVRYTSAENATDGAKTGLWLWLGFIVTTNLN
                     TVFFEFRPLGLYLINIGYHLVAFLAMGALLAVWRKRRSEAKAPAYV"
     gene            complement(6835..7212)
                     /locus_tag="VGB76_22550"
     CDS             complement(6835..7212)
                     /locus_tag="VGB76_22550"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0101723.1"
                     /translation="MPRVIHFELSADEPERAVRFYGEVFGWKTQKWDGPQSYWLLMTG
                     EAGQPGIDGGLMKRSDNPLPQASTTNTIDVPSVDDYTQKIKEHGGKVVMPKGAVPGIG
                     YVAYCEDTEGNLFGIMQFDPAAK"
     gene            complement(7661..8194)
                     /locus_tag="VGB76_22555"
     CDS             complement(7661..8194)
                     /locus_tag="VGB76_22555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101724.1"
                     /translation="MTTEELIQEQPLTFELEYENAPQAAVAAGVVEQENEETARVDQE
                     EARTKKEQILALYEAGTTDIAQIVRQVAARPSYVAQVLQSAGYLTGYFDLYTTTGREQ
                     NVYTRFFRNVLSFKTVDAARESVQKLDRLYNYFERLGDRAGQHQAMVLALTGKNRARW
                     SGKQEESEIFSEWLAAH"
     gene            complement(8294..9607)
                     /locus_tag="VGB76_22560"
     CDS             complement(8294..9607)
                     /locus_tag="VGB76_22560"
                     /inference="COORDINATES: protein motif:HMM:NF016024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HEY0101725.1"
                     /translation="MATATATNTTSTDKKAIMRDKRAQGIAAMGLVTREGDRFLVTTP
                     SLRGKRSSYEVWRDDAGKVRCSCLEFEEQSVEDPTFRCEHVLAVKHSLLARNTEAVTK
                     QQPADRTVARAAAPKTAETPNTNTTASEKRAPQGESRQADAATATTRFEREQDEPDTT
                     ADEGGETAAHTTAFAPAVSSLADHRQEEEQEEMSKRQATAVIEPEDDLEFVTAEEHAA
                     ATGSAPVVPLAFTSTLRTLRQPIDPKFIKTREGWRDRNGNPHMVEYVEWHTVADLLDR
                     IAPTWSHSVRGIVQIGDMVAVTAAISIDGVTREGVGTGTADNETGIKKAEHDALKRAA
                     IKFGIGRELYQRESEVIEQTGSAPQSEFPRDPLAKSMGDLVTPKQLGMIRALAREANV
                     DVEEECQQVLRCRTEELSKKAASSFIDHLKNLQGGEQQQPMRRAS"
     gene            complement(9638..9853)
                     /locus_tag="VGB76_22565"
     CDS             complement(9638..9853)
                     /locus_tag="VGB76_22565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101726.1"
                     /translation="MSDHIWETFDGLFKTVHRQGRRVEELEARIMELERRLAERAGAT
                     AAVAVNPVQQTKQAQPTVTEMPVERAA"
     gene            10059..10493
                     /locus_tag="VGB76_22570"
     CDS             10059..10493
                     /locus_tag="VGB76_22570"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0101727.1"
                     /translation="MAEFGKSLVNTVELGRAIRRKREEAGLSLRDVANETQVSASTLS
                     RIENGTGKPDADNIARLTAWLDMPMERIMSGRSQDADGNDAAKAVVYFPHEATPDIVE
                     AHLRADRNLSAETAKALSELFRVAYAQFSHPGEDPRTRNKRK"
     gene            10698..11264
                     /locus_tag="VGB76_22575"
     CDS             10698..11264
                     /locus_tag="VGB76_22575"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0101728.1"
                     /translation="MSSQRKTLMTPEEYLAAERKAETRSEYFAGEVFAMVGASKRHNL
                     IAANIIRVLGNQLLERPCNVYPSDMRVKVSATGKYTYPDVVVACEPEVFDDAENDTLL
                     NPVVIVEVLSASTEAYDRGKKFEQYQQIESLTEYILVAQEPYRIEQYVRQSDKEWRYS
                     EYHAAEEVIKISVIGGELALKDAYAKVV"
     gene            11297..11962
                     /locus_tag="VGB76_22580"
     CDS             11297..11962
                     /locus_tag="VGB76_22580"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEY0101729.1"
                     /translation="MDETKFPPTFKGRQYELRAMGLREFAGLRRDDEALDPFALAHFA
                     KLMVSDFEQVQGLSEETREHLLGVGSDAWSGGTCSRPLPNGWRLVLLNPRHGAARNNA
                     TLMEEVCHVFLGHKANRLSIVAQNKQGKTVARDYNEADEEAAYAVGAAALVPYSALSR
                     LVRAGKTSTEIARRFNVSRELVEYRMKVSRLWGEYRTQHAEEVEARYAARQAYANNNG
                     HAR"
     gene            11952..13124
                     /locus_tag="VGB76_22585"
     CDS             11952..13124
                     /locus_tag="VGB76_22585"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEY0101730.1"
                     /translation="MPVESPSPERERLLKLRDEIKRVIDYRSFYLRYCPNAHPPPAGG
                     RLQTLCPIPAHAHSGRGHPSLSVDLTRGLFHCFSREEGGDAIRFYELMHELKFSRAVR
                     ELGRELGLAGRGSRTVRALALRAAPDELPPVGQEPEPLAQERVSAICETFLAACRVED
                     QAEGLNYLARRGIDSGTARRAGVVYFPRRAYRRVMRRVQAAFAAEELQRSGLFNARAH
                     LTFYRHRLLFPFRVEGCAVYLQARTTAAGVEPRWHNMRGGVPSLYNVDALPELASGSV
                     VYLVEGFTDTLTLLAHNFAAVGLVGAGGLKAEWLAPLGRFRVVAALDPDAAGQRAACR
                     YEELFASRGLALARITLPADVNDFFRHHTAAALEFALLTEAALEKQESEHRIQDTE"
     gene            13280..14593
                     /locus_tag="VGB76_22590"
     CDS             13280..14593
                     /locus_tag="VGB76_22590"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY0101731.1"
                     /translation="MSEQIKDVSEATTATKLTVVDKLRMLLDITKKISRSLDVDEVLE
                     LVMDTLGTLLPYDAAGIYMIERSGRDDAPDGELGALKGFSTTSVRGYDINELTGLHLK
                     VGEGLLGYAAQTGEPLIVPDVSLDPRYVNARHDTCSEMVAPIISNDKVIGAFDLESDR
                     KNAYNEDDLEILMLLASQVAIIIEKAMLYEQSVEKKRLQAQLEVARQVQLELLPSRDP
                     RLEGFDISAYNFSTEEVSGDYYDFVSLYEDHLGIVIADVSGKGVPAALLMAFLRASLR
                     AAIHIGYAPNISMSKVNYLLWESIENNQYVTAFYGALDATNHTLAYANAGHNPPLLMD
                     AEGNARFIERGGIPLGMFRDTRYYEYYLPILAGQIIVLYTDGITEATNAAGTEYGRER
                     LEGKVREGRDLSARELINFLHADVLEWTGGRGASDDVTFVVIKAL"
     gene            complement(14746..15003)
                     /locus_tag="VGB76_22595"
     CDS             complement(14746..15003)
                     /locus_tag="VGB76_22595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101732.1"
                     /translation="MATYRDPVRVFAPLEVVERLLEIREEAGYSPEIFLRSSDDLAAY
                     FDAYGFDVYAFTETEAEFHYGRYRAALAPGGDHAHSSATAH"
     gene            15285..16550
                     /locus_tag="VGB76_22600"
     CDS             15285..16550
                     /locus_tag="VGB76_22600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY0101733.1"
                     /translation="MTTQQPPVPYATGTHSTEGSAPPAAAAPLNLVHVLQTMLGVSSK
                     VSDLIFSPGRPPQVELVGKLQGVHYPGLEKLTPAHTSGISKIIMARNPAAVESLEKFG
                     SADVSFSAPGLSRFRVNIFMQRGTHAIVMRVIPTEPPRWSNFNLPPQLKEIAELKTGL
                     VLVTGPTGSGKSSTLAAIIDLINEEKHYHIVTIEDPIEFLHSHKNCTIHQRELHSDTP
                     DFARALRAALRQAPKVILVGEMRDRETIEVALEAAETGHLVLSTLHTIDASKTVDRII
                     GVFPKNEEASIRARLAQTFRFIVSQRLVPRADKNGRIAAIEILKSTARTREYMEHGEK
                     EGKSLIDAMLQGELEGMQTFDSVLEKLIRNGTLNREEALSYASNQGNLRLQLADFDGT
                     PEAAPSPAKPAPSAPPKSSGNSMLDMLDR"
     gene            16707..18071
                     /locus_tag="VGB76_22605"
     CDS             16707..18071
                     /locus_tag="VGB76_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101734.1"
                     /translation="MRTRFTGRVVFVFVIIAASVAALFVASCGNKGSTRKGVPAASAL
                     APVRRELVPLPARPLSPALEGMRRRIVAAVADTRELDWQADAGMIELSGWEYGTRAKE
                     MADALAGDELRALSRLAIAGGMLPEGTDLATLAASFAASSAGATYSPLHKRVLILAGQ
                     GADAPAHDESLITHEYVHALQDQHFDLLKLLAARPYNFDRAEAAFAVVEGDAMNVQRR
                     REQTDAAWSRRTLEEIGQSEDARFAEYRKEIGAFFPPLLTETFIFRYRDGTRFVETVR
                     RKSGVRGIDELFRRPPASSEQVLHPEKYFAQESPREATLDEEAFAANNWKVAATTPLG
                     ELGVRGLLLKTLPAAEAARAAAGWGGDRAYLFERAKATPLFVWKTYWDKPEEAAEFFR
                     AYNTLRAKAGETRDGGGAGNPAEFIWRDSGGRLTIVRAEREQVLVIRGAEADARGALE
                     FARR"
     gene            complement(18225..19733)
                     /locus_tag="VGB76_22610"
     CDS             complement(18225..19733)
                     /locus_tag="VGB76_22610"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0101735.1"
                     /translation="MTHEVVVVGAGVGGLTCAALLAARGVNVCVLEKEPQGGGCATTF
                     ERFGYEFETTAGLYAGWGAGDIHTRIFAELPLAPAPETRRLSPAYIVRLPDRTEVCVG
                     GESGEGFETKLSDAFPECAAAAINFYRELTPIAETLERSARRMPDLATLTKFQRMRLM
                     ATEARHASRVLALQSHTAAQHLADASPRFRRFVDAQLQMFAGCASDACAYLYAAVALN
                     LPRRGLYAISGGASALARALTDSIKQSGGTLRFDTTALRLAFDPAGRAVGVTLLNGET
                     VSASRAVVSNLTVWDTYGKLVGLSRTPAALRERLKLARGWGAYLLYLGADEDALRRLP
                     ATHILALDSWQDEHETYDAAQGGIMFSASPAWDTKRAPAGKRAVTVSLYTDADEWFAY
                     HTDESEHEAQDQRFLEATWERLHVALPELGAGVEVIETATPRTFYEATRRKLGMVGGI
                     PQTLAASGANAFTHRTPFPALYLVGDTTFPGNGLAAVSQSALIVANEIAPRK"
     gene            complement(19739..>20142)
                     /locus_tag="VGB76_22615"
     CDS             complement(19739..>20142)
                     /locus_tag="VGB76_22615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY0101736.1"
                     /translation="IVAMAVLTAPLVEEVVYRGVLYPALQRRIGMHLGVVGVMLVFAA
                     IHFPQYYPNYGAISTIVVLSLTLTVIRAYTGRLLPCVVIHTIFNSASSVGILLEPYLK
                     NLQSLTEQPTSSFLPLLLSTSPVARLISYLF"
BASE COUNT         3829 a         6113 c         6147 g         4053 t
ORIGIN      
        1 tcactcgtca cttgtcactc gtcacttcta ccaatggcgt ttcggtggcg ggggcgggtt
       61 gcccggcagg cggtggcggc acctgtggag gcggcgcgcc gggctggtct ttttcctctg
      121 tggcgacggc ggaagcgtcc gccgcaggct ccgcgcgctc cgtgccttcg ggcgaaggaa
      181 tcacttcgat gagcgcgtga ttgtccgtgt gcaggtagcg cgtcaccacg tccttgatct
      241 gttccggcgt gacgctcagg taaagctcga tctccgtgtt gacgagatgc gggtcgccgt
      301 cgtagagcgt gtattcggcg agtcgttgcg cgcggtacat cgtggtctgg cgtccgcgca
      361 cggaatcgtt caagagcgtg ttgcgcagtt tctccatctc ttccgcgctc ggaccttctg
      421 tggcgaggcg tgcgatctct tcgtttatcg tgcggcgaat ctcttcggtc gtgcgtccgg
      481 gtttcgggat ggcgaacagg tagagcgacg aaggaccccg gcgttcgccg acgccgccct
      541 gcactgcgac gaccgattcg tcgcccttca cgagcttttg ataaagccgc gagctttcgc
      601 cttcgagcag gagttcgctg gcgagcgtta aggcgtaggc gtcgggcgtg cggcgcgcgg
      661 gcgctttcca gccgagcatc agcgcgggaa gctgcgcgaa cggatcggga tacttctcga
      721 agggcgcggc cacttccagc ggttcgctca cgtccacagg gggcggcgcg ggctggctcg
      781 gaatggtcgc aaaatatttc tccacgagcg cgcgtgcttc ccggctgtcg aagtcgccgc
      841 agatgatgag cacggcgttg ttcggcgcgt agtagatgcg aaagaactcc ttgatgtctt
      901 cgatggtggc atcgtcgagg tcttgcatgg agccgatggt cgagtgcgcg ttcgcggggt
      961 tccggtagat gagttcgttg aggcgcagga aggcgttaga gtaggggcgg ttgtcgtagt
     1021 tgagccgctt ctcctcctgc acggcattgc gttgattgtc gagattctcc tgcgtgacct
     1081 tcagagagcg catgcggtcg ctctccaacc agagcgcgag cgggagttga ttggccggga
     1141 gcgtctcgta gtagttggtg cgttcggatg aagtcgtgcc gttcatcgtg ccgccggaat
     1201 tgaagatgta ctggaagtgc gcggccttcg gcacgttctc cgagccttga aacatcatgt
     1261 gctcgaagag atgcgcgaaa cccgtgcgcc cctcgcgttc gttgcgcgag ccgacatcgt
     1321 aatagacgca gacggagacg acgggcacgc tccggtcttc gctcaagacg acgcgcaggc
     1381 cgttcgctaa ggtgaactgt tcgatgggaa gatgcggcag tgtgaagcta gcggacaaat
     1441 taagacctca gtataaattg aaatgataaa taacgatgac gtactgcgaa acggggcgac
     1501 cctctttctc cgccggttca aacttgatcc ggcgcgcggc cttgatcgct tcctcagtta
     1561 cgccgtgagg caaaccttcg atcaccttta tcttgtcacg cactttgccg tccgagccga
     1621 gaatgatgcg tagtttgacc acgccgcgca ccccgtagcc acgcgcctcg accggatagc
     1681 cggggttcgg cttcgacttg atgacggcgc gcttggtgac tcccctgacc gtctgcggtt
     1741 cgtcgtccgc ttgcgctcca tcttgcggct ggtcctgcgc cccgtctttc gcttcctctt
     1801 ttgtctcaga ctttccggcg cgctcttcct gcggcggctc actgcggcgc acgcggcgcg
     1861 attgcgcgag ggcgtccgcc gcgcacacag acgcgaaagc acagaagatg aaaaggacaa
     1921 aaacgcgttt cataatcttt cggacttttg ccgcccggaa gcgtgtaaag aagttgccgc
     1981 cagttccgcg ccgccgcgat ctctaacttt gcggcgtatt gatcatgccg cgcagttcgg
     2041 cgcgcagacg ctcgatctcg tggagtttct gctgcacgcg ttcgaagagc ggggtctgcg
     2101 atttgatgcc ttcgtcaatg agaaaggctg ccgcttcggc gcgcgagcgg aagactcccg
     2161 cctgcaccag gttctccagc ttctcctgtg cctcgtgcga aactttgacg gcaaccacgt
     2221 aatcgtcctt cgcagagatg gcgcgctcga tggtgcggct gatgtcgcgc ccgatggaag
     2281 agagcgattc agggatgcgc gaggcgatgt cgcgcacgga agaggacatc cgttcgcggt
     2341 ctgcgccgtg ccccgtttcg tcttcgtctt cgcgcagcat caagtcgtca atcatgtcgc
     2401 tctgattcac cggggagggg tcgtccgggc tatgcgggtt gtttgggtca taagggctgc
     2461 tcatacgata aatctccttc acgtctgttg acgccgcgca cgcgggctta aaagtaacga
     2521 ccgggcgcgt gcctgcttcc tgtgcaatgt ggacgttgat tgccgcaccc attttaagtt
     2581 atcggaggga aagtaacaaa cagccgaccc ggcgagaaga agggaaaatg gtaaatgcta
     2641 aaggggaagg gaggagagca gatgcgtcta ctccaaccag cccttttcct ttccctttcc
     2701 gcccgcgcct ttcccccttt ttctcccgcc tcgtacgaga aaatggatgc gcctttgccg
     2761 gagcgggacg caaccttgcc gccctttcac tcatccagta ctatacaaaa actgcctccg
     2821 gcggtcttgg tcgcacaccc ggcgattacg cttccggcgt ttatttgatt tcaacttaag
     2881 cggaaatggt cttgaaaggt ttgccagcag tgaaacaaaa aatttcatcc gccctcgtcg
     2941 cgcttctact cgtcgcgctt tcgcttccca cctctgttct cgcgcagcag cctgcgcctg
     3001 aacgtcgtgg caccgctccc ggtcagagca tgcccccgaa cgttggcgcg cccgctcaga
     3061 ggggcacagg gcgacaacgc cagcgcgtcc cggcttcgtc cggcgcgacc gccgccatcg
     3121 aagaggactt cgcagaggct ttgagcatcg tgcaggaaaa ctatgttgac ggcgtgaaga
     3181 tcgattacaa cgccgccttc aagtcgtcca tcatcggcat gctgcgctcg ctcgacccgc
     3241 actcgaacta ttacgactcg aaagagttcg aggagctaca ggccgactgg cgttccgagt
     3301 attacggcat cggcgcgacc atcggcgacc gcaccatcgc cggtcagacg gatacctaca
     3361 ttctcgccac ttttcccggt gcgcccgcca cgcgcgctgg cctgcgcttc gccgaccgca
     3421 tcgtcgaagt gaacggcgag gccatcaaag gcaaaaattc gagcgacgtg cgcgaccgcc
     3481 tgcgtggtcc gaaaggctct accgtcaagg tcacggtcga acgcgctgcg acgaagacgc
     3541 tggagacggt cgagattacg cgcgacgccg tcggccagcc gacggttccc gattcctaca
     3601 tgatcaagcc ctctgtgggc tatgttgaca tgacgcgcgg cttcaatcgc agcacagcgg
     3661 aagagttctc cgccgcactc gaagacctgc acaagcaggg catgacctcg ctcgtgctcg
     3721 acctgcgcgg caatccgggc ggacttctcg atcaggccgt caaagtggcc gagcgatttt
     3781 tgcagcgcgg gcaggtcatc ctttcgcaga aagggcgcgt ccccggcagc gagcgcactt
     3841 atgaatcgcg caacagttcg cccgattccg tgccgctcgt cgtgctcgtc aaccgcaact
     3901 ccgcttcggc ctccgagatc gtggcgggcg cattgcagga ccacgaccgc gcactgatca
     3961 tcggcgagac gagcttcggc aagggactcg tccagagcat catcccgctc gaatacggca
     4021 cggcggtgac gctcacttcc tcgaagtatt acacgccttc gggtcgcctc atccagcgcg
     4081 attacacgaa cgaaggactc tacgattact acacgcgcgg cggcgtcggt agtctcgacg
     4141 cggacaacaa agcggcggag cctgccaagc cctccggccc cgagagtctg accgataccg
     4201 ggcgcaaagt ttacggcggc ggcggcatcg cgcccgacga acccgtcaaa ccgcgcatca
     4261 tcgaaccggc gcaacaacgc ttaaccgatc ccatcttcgc tttcgtgcgt gaactcgtca
     4321 acgggcgcat caagggtttt gagaactaca aagtcgcgcg cgccattgat tacaatcacg
     4381 acataaaagc cgaggactac ccggtgacgg ataacttgtt caaggcgttc aagacgttcg
     4441 tggcgaacga cgagacgctt aaagtgaaag aagcgcaact tgataagagc cgcgagttca
     4501 tcacgcgcca gatgcgttac gacatcgcca ccgccgctta cggcacggtc acggcgtcgc
     4561 aagtgctggt cacggacgac ccgcaagtcg ccaaagcgat agacgtgctg ccgcgtgcgc
     4621 gcgacctctc gacggcagcc atgcgcgggc gcaatcccga acgcaagacg tttgaataac
     4681 acgcgccagc cgctcgcagg ctttaatgaa agaggccgtc aactgcaaaa gttggcggcc
     4741 tcttcgcttg tttgcttttc acccttcgcc gcagaggcac cagtttatta ctctcccgtc
     4801 cggtcgtgcg gcgcatcgac gggcttggat tcgggtgagc ctttctgtcg cagccagacc
     4861 gtgagcagca ccatcatgcc gatggcgaga aattcgctct gccagttttg tagcgactcg
     4921 aaccagaagc gtgaagtgcc catgtaggcg agggcggaaa cctgttcgcc cgcgttgccg
     4981 tgcgcgatct gttcctgatt gtattcgccc gcgccgccca cggcgtgcag ccagaaagag
     5041 atgaggaaca gcagaaagaa agtgagactc agcgagttct cataaagttt caggacgaag
     5101 ccgccgcgac gcaccggcca cggcgcatcc tttttgttgc gcgagttgcg cgggtcgcgg
     5161 tctacggcct cctcttcgtc cgggtctttc gattccgctg agcctttctg gtagaggaag
     5221 atcgtgagca cgatgaagat gaacatctgt agaaactcgc tctcccagtt ctccatcgtc
     5281 gcttcgagaa agtggggggt ccagaggtat tcggcgtaac cggcggcggg ttggccgtgt
     5341 gcctgctggt cgttgttgta ttcgcgctgg ccgacgactg attgaccgac gataagcgtg
     5401 aagaagaaca gcacgaacag cacgagcgac agcccgttgt tgcgaaaaaa tcgcctcata
     5461 agttttctct tcgtcgttct cgaacggtaa gattttacgt cagagcttgg gctaatcctg
     5521 cttggcgaac tgtcgagttt gcggctgcgg cttcggttcg tcgtggccgg ctcgctgctt
     5581 taattcgtcg aaattgatct cgtttgaagg gcgcgtgtcg gccttgagca cgcgctgcat
     5641 gtgttcgagg gcgtggcggt tctcgtctgc gtcgctggag gcgacacagt cggcggggat
     5701 caggagatgg aagtcgcgca tgtaggcgtc gttggcggtg aagaggacgc agatgtcgcc
     5761 ggtgaggccg gtgaggatta agctttttac ttgcaggtaa tcgagcaggg tgtcgagggt
     5821 ggtggagaaa aatccggagt gcttgggctt gaggacgaag tagtcgtctt cctggggctg
     5881 caacagttcg gcgacgggtc tgccgcgcac gtcctcttcg agacaatgct tgagcacgga
     5941 tttgaagtcc gattgccact tcccgaaatt gtcgttgacg tagatgacgg gaatcttcgc
     6001 ttccttcgca cgctgtttca acgcggccaa cttctcggcc atcggcagcg catgttcgag
     6061 caatttcccg ccgctgctga actccagatc gttgatgaca tcaattaaca gcaaggccac
     6121 ttccgccttg tccggcacaa acccgtgcaa gtcttcattc ttcgccggca cgctttcgtc
     6181 tagccccctt cgcacgtcga attccaccga gcaatcaaac tcgcgtgcat agtacccgaa
     6241 cttccggcga aaaggggagg ggaaaatcag ccggtggccg tgtgttcgtt gtttagcgac
     6301 cgtcacacag gttactaaag attcggcttg aaccaacggc caccggccat atcacacgta
     6361 agcgggagct ttggcttcgc ttcgccgctt gcgccagacg gccaacagcg cgcccatggc
     6421 gagaaaggcg accagatggt agccgatatt gatgaggtag aggccgagcg gcctgaactc
     6481 gaagaagact gtgttcaagt tggtggtcac gataaagccg agccacagcc acaagccggt
     6541 cttcgcgccg tccgtcgcgt tctccgcgct cgtatagcgc acgatatgcg ccagcacgta
     6601 agccgcgacg agtgagccga cgaaggccaa cgccagttgc gcccccgcgc cttcggcctg
     6661 aacgcgcgca atctgctccg gcgaccagcg catgacctcg atgaacttgc ccccgaacag
     6721 gagcggtgaa taccagattc cgccgagcac ccagtatcca atcgccgaga cggctaccgc
     6781 cgcgtagtta atcttcatca catttctctc ccgtttaaag ttggacacgc acgctcactt
     6841 ggccgccggg tcgaattgca tgatcccgaa gaggttgccc tccgtgtctt cgcaataagc
     6901 gacgtaaccg atgcccggca ctgcgccttt cggcatgact actttgccgc cgtgctcctt
     6961 gatcttctgc gtgtaatcgt caaccgaagg cacgtcaatc gtgttggtgg tggaggcttg
     7021 cggcagaggg ttgtcggaac gtttcatcag cccgccgtcg atgccgggct gtcccgcctc
     7081 gcccgtcatc agcagccagt agctctgggg gccgtcccac ttctgcgtct tccaaccgaa
     7141 gacctctccg tagaatctca cggcgcgctc gggttcgtcc gcgctgagtt cgaaatgaat
     7201 aactctcggc atgttgttct ccttcatcgc ttgggaaatt ttcacttcca ggccgtgcgc
     7261 ctctttgaga gacgaaggca cggcattgat tatgcgaggc gatgtgtggc ctgtgccgga
     7321 aacgaggacg ccgcttttat tactacggtg ttcgagcggc gcaaggccgt gggcttggcg
     7381 cgttgccaaa ccgcagcgag ggtcttcttt tgtggcgagg gtgtaacgcg accgtcgaag
     7441 cgagcgttgc gcgttttcag tctggaaaag cgcgtggact cgccggtcgc ctgtcaaagt
     7501 ctggattgct gaatagctgg agattgcctt cgggataaaa agaaaggtga gttaacccct
     7561 tgttcgtaac tcacctttca acccgccctg tgttcgttct ttgcgcgcaa ctcgtggttg
     7621 acgttaattt cttccactcg acgacgcggc ttcttccacg tcagtgagcg gcgagccact
     7681 cgctgaatat ttcggactct tcctgcttgc cgctccagcg cgcgcgattc ttgcccgtca
     7741 aagcgagcac catcgcctga tgctgtccgg cgcggtcgcc gagccgctcg aagtagttgt
     7801 agagccggtc gagtttctgc accgactcgc gcgccgcgtc caccgtcttg aacgagagga
     7861 cgttgcggaa aaagcgcgta tagacgttct gctcgcgccc cgtcgtcgtg tagagatcaa
     7921 agtagcccgt caggtatccg gcggactgca agacctgcgc cacgtaactc ggacgcgccg
     7981 ccacctgcct gacgatctgc gcgatgtccg tcgtgcccgc ttcgtagagc gcgagaatct
     8041 gctccttctt cgtgcgcgct tcctcttgat ccacgcgcgc cgtctcttcg ttctcctgct
     8101 cgacgacacc agccgccacc gctgcctgcg gcgcgttctc gtattcgagt tcgaacgtca
     8161 gaggctgttc ctgaatcaat tcttctgtcg tcatctttaa cctcttactt cccggagccg
     8221 ccgcgcgcgg ggcaggtttg attcaatcat tgccgccccg ccgcgactat ccaggctccc
     8281 cttggtaact ccgttagctg gcgcggcgca tcggctgttg ctgctcgccg ccctgcaagt
     8341 tcttcaagtg gtcgatgaag gacgaagcag ccttcttcga caactcttcc gtgcggcagc
     8401 gaagcacctg ctggcactcc tcttccacat ccacgttcgc ctcgcgtgcg agcgcgcgaa
     8461 tcatgccgag ttgcttcggc gtcacgaggt cgcccatgga cttcgccaga ggatcgcgcg
     8521 ggaactcgct ctgcggagcc gaaccggtct gctcgatcac ttccgactcg cgctggtaca
     8581 actcgcgccc gatgccgaac ttgatggccg cacgcttcaa cgcgtcgtgc tcggccttct
     8641 tgatgcccgt ctcgttgtcc gccgtgcccg tgccgacgcc ttctctggtc acgccgtcaa
     8701 tcgagatggc ggcggtgacg gccaccatgt cgccgatctg gacgatgccg cgaaccgagt
     8761 gcgaccaggt gggcgcaatg cggtcgagga ggtctgcgac cgtgtgccac tcgacgtatt
     8821 ccaccatgtg cgggttgccg ttgcggtcgc gccaaccttc gcgcgtcttg atgaacttcg
     8881 ggtcaatcgg ctggcgcaga gtgcgcagag tgctcgtgaa agcgagcggc acaaccggcg
     8941 cgctgcccgt cgctgctgcg tgttcttccg cggtgacgaa ttccagatca tcttccggct
     9001 cgatcacggc agttgcttgt ctcttactca tttcttcttg ctcctcttcc tgtctgtgat
     9061 ccgcaagcga actcaccgcc ggggcgaacg ctgttgtgtg ggcggctgtc tcgccgcctt
     9121 cgtccgccgt cgtgtccggt tcgtcctgct cccgttcgaa acgcgtggtc gccgtcgcgg
     9181 cgtcggcctg tcggctctca ccttgcgggg cgcgcttctc ggatgcggtc gtgttcgtgt
     9241 tgggcgtctc cgccgttttt ggcgccgccg cgcgtgcgac ggtgcggtct gccggttgct
     9301 gcttggttac agcttccgtg ttgcgtgcga gcagcgaatg cttgacggcc agaacgtgct
     9361 cgcaacgaaa cgtcgggtct tcgaccgact gctcctcaaa ttccagacaa gagcaacgca
     9421 ctttgcccgc atcgtctcgc cagacttcgt agctgctgcg cttgccgcgc aaggagggcg
     9481 tcgtcaccag aaagcggtcg ccctcgcgtg tcaccaagcc catcgccgcg atgccctgcg
     9541 cgcgtttgtc gcgcatgatc gctttcttgt cggtgctcgt cgtgttggtc gcggttgctg
     9601 ttgccatggt ctttaactcc ttgaaaatcc tttaacttta cgccgcgcgc tctacgggca
     9661 tctccgtcac cgtcggctgc gcctgcttcg tctgctgcac cggattcact gctactgccg
     9721 ctgtcgctcc ggcgcgttcc gccagccgac gctcaagctc catgatgcgc gcttccaatt
     9781 cctccacgcg ccgcccctgc cggtgcaccg tcttgaacaa tccgtcgaat gtttcccaga
     9841 tatggtcgct catctcaccc tcaacttttc gggctgtttt cgccgtgtct gaaacgctct
     9901 gtttcatacg tgaaacagtg taccatgaga cgttttttgt tgcaagcata aaacaaggtc
     9961 ttgcgtctgg gattttcctg tgatatggtc tgggattaga ggaggggcgt cttgcggctt
    10021 cctgtaagct gtcggacgca tcaagaagga ggccaaacat ggcagaattt ggaaaaagtc
    10081 tggtaaatac ggtggaatta gggcgtgcga tccgtcgcaa acgcgaggag gcgggcttga
    10141 gcctgcgcga cgtagcgaat gaaacgcagg tgtcggcctc gacgctctca cgcatcgaaa
    10201 acggcacggg caaacccgac gccgacaaca tcgcgcgcct aaccgcgtgg ctcgacatgc
    10261 ctatggagcg catcatgagc ggtcgctcac aggatgccga cggcaacgac gcagccaaag
    10321 cggtcgtcta cttcccgcac gaagcgacgc ccgacatcgt cgaagcgcac ctgcgcgccg
    10381 accgcaatct ctcggcagag acagccaaag ccctctccga actcttccgc gtcgcctacg
    10441 cccaattcag ccatcccggc gaagacccac gcacgcgcaa caagcggaag taaacggcac
    10501 gcacagttct caagtttatt aggcgctcaa cccttccgag gtgagcgcct aatttaatta
    10561 tttcattaaa ctgttggtga aatgatgttg tgagcgggga aagtttgttt tgacagtagg
    10621 ctcgatttac agcgtaaatt tcagcggagc aaagtggtgt ctaagcgggt ataatcttct
    10681 taagcgaaag gagccgcatg tcttctcaac gtaaaacctt aatgacgccg gaggaatacc
    10741 tcgctgcgga gcggaaggct gagacgcgga gcgagtattt cgcgggtgaa gtgtttgcga
    10801 tggtgggggc gagtaagcgg cataatctca tcgcggcgaa tatcatccgg gtgctaggca
    10861 accagttgct cgaacgcccg tgcaatgtct acccgagcga tatgcgtgtg aaggtgagcg
    10921 cgacggggaa gtacacttat cctgatgtcg tggtggcgtg tgaaccggaa gtgtttgatg
    10981 atgcggagaa tgatacgctg ctcaatccgg tggtaatcgt cgaagtcttg tcggcttcga
    11041 cggaagctta tgatcgcggg aagaagtttg agcagtatca gcagattgag tcgctgacgg
    11101 aatatatcct tgttgcacaa gagccgtacc ggatcgagca gtatgtcagg cagagcgata
    11161 aagagtggcg atattcggaa tatcacgctg cggaagaggt aataaaaatt agcgtcatcg
    11221 gcggcgagct tgctttgaag gacgcgtatg ccaaagttgt gtgaactgga gccgcttggg
    11281 attagggacc gcgcaaatgg acgagacgaa atttccgccg acgtttaagg ggaggcaata
    11341 tgagttgcgg gcgatggggc tgcgcgagtt tgcggggttg cgtcgtgatg atgaggcgtt
    11401 agacccgttt gcgctggcgc attttgcgaa gttgatggtg agtgatttcg agcaggtgca
    11461 ggggttgtcg gaggagacgc gggagcatct gttgggggtg ggttcggatg cgtggtcggg
    11521 cgggacgtgt tcgcgcccgc tgccgaacgg gtggcgtctg gtgctgttga atccacgtca
    11581 cggcgcggcg cgcaataacg cgacgttgat ggaggaggtc tgccacgttt ttctcggaca
    11641 caaggcgaac cggctttcaa tcgtggcgca gaacaagcag ggaaagacgg tcgcgcgtga
    11701 ttacaacgag gcggacgagg aggcggctta tgcggtgggc gcggcggcgc tcgtgcccta
    11761 tagcgcgctc agccgtctgg tgcgcgcagg caagacttcg acggagatcg cgcggcgttt
    11821 caatgtgagt cgtgaactgg ttgagtatcg catgaaggtg tcgcggctgt ggggcgagta
    11881 tcgcacgcag cacgcggaag aggttgaggc gcgttacgcg gcgcgtcagg cttacgcgaa
    11941 caacaacggc catgcccgtt gagtcgcctt cacccgagcg cgaacgcctg ttaaaactgc
    12001 gcgacgagat caagcgcgtc atagattatc gatcctttta tttacgctac tgtccgaacg
    12061 cacaccctcc tccagcgggt gggcgtctgc aaacgctctg ccccatcccc gcacacgcgc
    12121 atagcggcag aggccatccg agtctctccg ttgatctcac gcgcggcctc tttcattgct
    12181 tctcgcgcga agagggcggc gacgccatac gcttctacga gttgatgcac gagttgaagt
    12241 tcagccgcgc cgtgcgggaa ctcggacgcg aactcggact ggccggacgc ggcagccgca
    12301 ccgtgcgcgc tctcgcgctg cgcgccgcgc ccgacgagtt gccgccggtg gggcaagaac
    12361 cggagccgct cgcgcaggaa cgtgtcagcg cgatttgtga gacttttctg gcggcgtgtc
    12421 gtgtggaaga tcaggcggaa ggtcttaatt atctcgcgcg gcgcggcatt gacagcggga
    12481 cggcgcggcg cgcaggcgtg gtctatttcc cgcgccgcgc ctatcgacgc gtgatgcgcc
    12541 gggtgcaggc ggcgttcgca gccgaagagt tgcagcgcag cggtctgttc aacgcgcgtg
    12601 cgcacctgac gttttatcgt caccgcctgc tgttcccgtt tcgcgtggag gggtgcgcgg
    12661 tctatttgca ggcgcgtacg actgcggcgg gcgtcgagcc gcgctggcat aacatgcgcg
    12721 gcggcgtgcc ttcgctttat aacgttgacg ctttgcccga actcgccagc ggcagcgtcg
    12781 tctatctcgt tgaaggtttt accgacacgc tgacgctttt ggcgcacaac tttgcggcgg
    12841 tcggactcgt cggcgcgggc ggcctgaagg cggaatggct cgcgccgctc ggacgtttcc
    12901 gcgtcgtcgc cgcgctcgac cctgacgcgg cgggacaacg cgcagcctgt cgctacgaag
    12961 aactcttcgc ctcgcgcggc ctcgccctcg cgcgcatcac gctccccgcc gatgtcaacg
    13021 atttctttcg acaccatacg gcagccgcgt tggagttcgc actgctgacc gaagcagcgt
    13081 tggaaaagca ggagtcagaa cacagaatac aggacacaga atgaaagcaa cttatttctc
    13141 ttctgacttc tgtattctgt gttctgtatt ctctcttcat tcatccgtca tcgcttctta
    13201 tccttcgtcc ctgctctcgc atcacttata atcaggtttc gcttaagtgc cggtgtgcgc
    13261 cggaagcgta tgtcccttta tgtctgaaca gatcaaagat gtgtcggaag cgacgacagc
    13321 aacgaaatta accgtcgtcg ataagctgcg gatgctgctc gacatcacga agaagatcag
    13381 ccgctcgctc gacgttgacg aggtgctcga actcgtaatg gacacgctcg gcacgcttct
    13441 cccttacgat gcggcgggca tctacatgat cgaacgcagc gggcgcgacg acgcgcccga
    13501 cggcgaactc ggcgcgctca aaggtttcag cacaacctcc gtgcgcggct acgacatcaa
    13561 cgaattgacc gggctgcatt tgaaggtcgg cgaaggtctg ctcggctacg cggcgcagac
    13621 gggcgagcct ctgatcgtgc ccgacgtgag cctcgacccg cgttacgtca acgcgcgaca
    13681 cgacacctgc tccgagatgg ttgcgccgat catctcgaac gacaaagtga tcggagcttt
    13741 cgatctggag agcgaccgga agaacgctta caacgaagac gatctcgaaa tcctgatgct
    13801 gctcgcctcg caggtcgcca tcattatcga gaaggccatg ctgtatgagc agtcggtcga
    13861 gaagaaacgc ctgcaagcgc aactcgaagt cgcgcgtcag gttcaactcg aactcctccc
    13921 ctcgcgcgac ccgcgactcg aaggcttcga tatcagcgcg tacaacttct caaccgaaga
    13981 agtctccggc gactactacg atttcgtcag tctctacgag gatcatctcg gcatcgtcat
    14041 cgcagacgtg tcgggcaagg gcgtgcccgc cgcgctcctg atggccttcc tgcgcgcgag
    14101 cctgcgcgcc gccatacaca tcggctacgc gccgaacatc tcgatgtcga aggtcaatta
    14161 tctgctctgg gagtcaatcg agaacaatca atacgtgacg gccttctacg gcgcgctcga
    14221 cgcgacgaac cacacgctcg cttacgccaa cgccgggcac aacccgcccc tcctgatgga
    14281 cgcggaaggc aacgcgcgct tcatcgaacg cggcggcatc ccgctcggca tgtttcgcga
    14341 cacgcgctac tacgaatact acctgcccat tctggccggg cagatcatcg tgctctacac
    14401 ggacggcatc acggaggcga ccaacgcggc gggcacggag tacgggcgcg agcggctcga
    14461 aggcaaggtg cgcgaaggac gcgacctgag cgcgcgcgag ttgatcaact tcctgcacgc
    14521 cgacgtgctc gaatggacgg gcgggcgcgg cgcgagcgac gacgtaacct tcgtcgtcat
    14581 caaggcgctc tgaaacgatg aaggaggaac gatgaaccgg aatacagaac acagaattca
    14641 ggaatcagca tgaagactcg tgcaggcttt attctgagcg gaacggggcg tcgagtttaa
    14701 gctcgacgcc ccgttccggt taaatttgtt tgatgggttg gaaaatcagt gtgcggtcgc
    14761 tgaactgtgc gcgtggtcgc cgccgggcgc gagcgcggca cgataacggc catagtgaaa
    14821 ttcggcttca gtctcagtga aagcgtaaac gtcgaagccg taagcgtcga agtaagctgc
    14881 caagtcatcg gacgagcgga gaaaaatttc gggagagtaa cccgcttctt cgcgaatctc
    14941 cagcaggcgc tcaaccactt ccagcggagc aaaaactctc acagggtctc tataggttgc
    15001 cataaacttg cgcgcctcct tctggtcagc cgggatgccg tcagttgaac ctctagcaca
    15061 tcccgtgcct tgactctaat ttgatgtacg ccggaaaggg cgcggttgtt tgctccctgc
    15121 gtatattttt cgttacaggc tttacgaaag tttgttccga acggtacagt attagtgtcc
    15181 agcgtgcgcg gccagccaaa aggccgcgcg ataaactcac ggacgcccag caccccgaca
    15241 tattctccaa aatttcggaa catctttaac gactcacaaa atttatgacg actcaacagc
    15301 cgcccgtccc atacgcgacg ggcacacact cgaccgaagg cagcgcgccg ccagccgccg
    15361 ctgctccact caatctcgta cacgtcctcc agacgatgct cggcgtctcc tccaaggtca
    15421 gcgatctgat cttctcgccg gggcgtccgc cgcaagtgga actcgtggga aaactacagg
    15481 gcgtccacta tcccggactt gaaaaactga cgcccgcgca cacctccggc atctcgaaga
    15541 tcatcatggc gcgcaatccg gcggcggtcg agagtctgga gaagttcggc tcggcagacg
    15601 tttctttcag cgcgcccggc ctctcgcgtt ttcgcgtcaa catcttcatg cagcgcggca
    15661 cgcacgccat cgtcatgcgc gtcatcccga ccgagccgcc ccgttggagc aacttcaatc
    15721 tgccgccgca actcaaagag atcgccgaac tcaaaaccgg cctcgtcctc gtcacaggcc
    15781 cgaccggctc cggcaaatcc tccacgctcg ccgccatcat cgatctcatc aacgaagaga
    15841 agcattacca catcgtcacc atcgaagacc ccatcgagtt tctacacagc cacaagaact
    15901 gcacgatcca ccagcgcgaa cttcactcgg acacgcccga ctttgcgcgc gccctgcgcg
    15961 ccgcgctgcg ccaggctccg aaggtgatcc tcgtcggcga gatgcgcgac cgcgagacga
    16021 ttgaagtcgc gctcgaagcc gcagagacgg ggcacctcgt gctctccacc ctgcacacga
    16081 ttgacgcctc aaaaaccgtc gaccgcatca tcggcgtctt tccgaagaac gaagaggcga
    16141 gcatccgcgc gcgtctcgcg cagaccttcc gtttcatcgt ctcgcagcgt ctcgtcccgc
    16201 gtgcggataa gaacgggcgc atcgcggcga tagagattct caaatccact gcgcgcacgc
    16261 gcgaatacat ggagcacggc gagaaggaag gaaagtcgct catcgacgcc atgctgcaag
    16321 gcgaactcga aggcatgcaa actttcgact cggtgctgga aaagttgatc cgtaacggca
    16381 cgctcaaccg cgaagaagcc ctgtcttacg cctccaatca gggcaatctc cgcctccaac
    16441 tcgcagactt cgacggcacg cccgaagcag ccccttcacc cgccaaaccc gccccctccg
    16501 cgccgccgaa gagcagcggc aactcgatgc tcgacatgct cgaccgctaa aacaggggaa
    16561 gggcaggcgg cgtgggaaaa gtgaaactaa gaaagcccat cccgttatag ttaaactttt
    16621 cctgctccct gcaagccctc cccttttccc gctcccctct gaatttaccg gcatcgcgga
    16681 gaggcgttcg cgcgtctaaa tttcgtatgc ggacaagatt tacagggcgc gttgtttttg
    16741 ttttcgtcat catcgcggcg agcgtggcgg cgttgttcgt cgcttcgtgc ggcaataaag
    16801 gctcgacgcg gaagggcgtc ccggcggcct ccgcgctcgc gcccgtgcgg cgagaacttg
    16861 tgccgctccc tgcgcgccct ctgtcgcccg cgttggaagg catgcggcgt cgcatcgtcg
    16921 cggcggtcgc ggacacgcgt gaactcgact ggcaggcgga cgccggaatg atcgagttgt
    16981 ctggttggga atacggcacg cgcgccaaag agatggccga cgcgctcgcc ggagacgaac
    17041 tgcgcgcctt gagccgcctc gccatcgcgg gcgggatgct gcccgaaggc acagacctcg
    17101 ccacgctcgc cgcgagtttc gccgcttcct ccgcgggcgc gacctactcg ccgctccaca
    17161 agcgcgtgct catcctcgca ggtcagggcg cggacgcccc cgcgcacgac gaatcgctca
    17221 tcacacacga atacgtccac gcgttgcaag accagcactt cgacctgctg aaactgctcg
    17281 ccgctcgccc ttataacttc gaccgcgcgg aggccgcttt cgccgtcgtc gaaggcgacg
    17341 cgatgaacgt gcagcgacga cgcgagcaga ccgatgccgc gtggtcgcgt cgcacgctcg
    17401 aagagatcgg gcagagtgaa gacgcgcgct tcgcagagta tcgcaaagag atcggcgcgt
    17461 tcttcccgcc cctgctgacc gagacgttca tcttccgcta tcgcgacggc acgcgcttcg
    17521 tcgaaaccgt gcggcgcaag tccggcgtgc gcggcataga cgaacttttc cgtcgcccgc
    17581 ccgcatcgag cgagcaggtg ctccatcccg aaaaatattt cgcgcaggag tcgccgcgtg
    17641 aggcgacgtt ggatgaagaa gcgtttgcgg cgaacaattg gaaggtggca gcgacgacgc
    17701 cgctcggcga actcggcgtg cgcggcctgt tgctgaaaac gctcccggca gcggaggccg
    17761 cacgcgcggc ggcgggctgg ggcggcgacc gcgcctacct gttcgagcgc gccaaagcca
    17821 caccgctttt cgtctggaag acttactggg acaagcccga agaggccgca gagttcttcc
    17881 gcgcttacaa cacgctgcgc gccaaagcgg gcgagacgcg cgacggcggc ggcgcgggca
    17941 accccgccga gttcatctgg cgcgacagcg gcggacgcct gacgatagtt cgcgccgagc
    18001 gggagcaggt cttggtcata cgcggcgcgg aagcggacgc acgcggcgcg ttggagtttg
    18061 cccgccgtta gccggaaata gaaaaccttc aacgcagaga cgcggagagt caacacgaga
    18121 ggggcgcaga gaaaagatta attcttctct gcgcccctct cgcttgcccg cgcgctcact
    18181 ctctgcgtgt ctctgcgcct gtccgggtga ctccgaatct ctccttactt tcgcggcgcg
    18241 atctcgttgg cgacgatgag tgcggattga ctgacggcgg cgaggccgtt gccggggaaa
    18301 gtcgtgtcgc cgacgaggta gagcgcgggg aagggcgtgc ggtgcgtgaa ggcgtttgcg
    18361 ccggaggccg cgagcgtctg cgggatgccg ccgaccatgc cgagtttgcg ccgcgtcgct
    18421 tcgtagaaag tgcgcggcgt cgccgtctcg atcacttcca cgcctgcgcc gagttcgggc
    18481 agcgcaacgt gtagacgttc ccatgtcgct tccagaaatc tctggtcttg tgcttcgtgc
    18541 tcgctctcgt ccgtgtgata agcgaaccac tcgtccgcat ccgtgtagag cgagacggtg
    18601 acggcgcgct tgcctgcggg ggcgcgcttc gtatcccacg cgggcgacgc gctgaacatt
    18661 attcccccct gcgccgcatc gtaagtttcg tgttcgtcct gccaactgtc gagcgcgagg
    18721 atgtgcgtgg cgggcagacg acgcaaggca tcttcatccg cgccgagata gaggagatat
    18781 gcgccccagc cgcgcgccag tttcagacgc tcgcgcaaag cggcgggcgt gcgcgagagg
    18841 ccgacgagtt tgccgtaggt gtcccagacg gtgagattgc tgacgacggc gcgcgacgca
    18901 ctgacagtct ccccgttcaa gagcgtgacg ccgacggcgc ggccagccgg gtcgaaggcg
    18961 agtcgcagcg cagtcgtgtc gaaacgaagc gtgccgccgc tttgtttgat ggaatcggtg
    19021 agcgcgcggg cgagcgcgga cgcgccgccg cttatcgcgt acagtccgcg acgcggcagg
    19081 ttgagcgcga cggcggcgta caggtaagcg catgcgtcgc tcgcgcaccc ggcaaacatt
    19141 tgcagttgcg catcgacgaa gcggcggaag cgtggcgaag cgtcggcgag atgctgcgcc
    19201 gccgtgtgcg actgtaaggc gagcacgcgc gaggcgtggc gcgcttcggt cgccatgagc
    19261 cgcatgcgct gaaacttcgt gagcgtcgcg agatcgggca tgcggcgcgc gctgcgttcg
    19321 agagtttcgg cgatgggcgt gagttcgcga taaaagttga tcgcggcagc ggcgcattca
    19381 ggaaacgcat cgcttaattt cgtctcgaag ccttcgccgc tttcgccgcc cacgcacacc
    19441 tccgtccggt cgggcaggcg cacgatgtag gcgggcgaga gcctgcgcgt ttcgggcgcg
    19501 ggcgcgagtg gtagttcggc gaagatacgc gtgtggatgt cgcctgcgcc ccaacccgca
    19561 tagagtccgg cggtcgtctc aaactcgtag ccgaaccgct cgaacgtcgt cgcgcagccg
    19621 ccgccctgcg gctctttttc cagcacacac acgttgacgc cgcgcgccgc cagcaacgcg
    19681 gcgcacgtca acccgcccac gcccgcgccg acgacgacca cttcgtgtgt cataaagttt
    19741 agaagaggta agagatgaga cgggcgacgg gagaggttga gagcaacagt ggcagaaatg
    19801 aagaggtcgg ttgctccgtg agcgactgga ggtttttcag atacggctca agcaggatgc
    19861 cgacggacga ggcgctgttg aagatcgtgt gaatgacgac gcaggggagc agtcgtccgg
    19921 tataagcgcg gatgaccgtc agcgtcaggc tcaggacgac gatggtggag atcgcgccgt
    19981 agttcggata atactgcggg aagtgaatgg ccgcgaagac gagcatcacg ccgacgacac
    20041 ccagatgcat gccgatgcga cgttgcaggg cgggatagag cacgccgcga tagacgacct
    20101 cttcgacgag cggcgcggtc agtaccgcca tcgctacgat gg
//
LOCUS       DASWNS010000653         8185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_258948, whole genome shotgun sequence.
ACCESSION   DASWNS010000653 DASWNS010000000
VERSION     DASWNS010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258948"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VGB76_22620"
     CDS             <1..327
                     /locus_tag="VGB76_22620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101737.1"
                     /translation="AVRLRVRFDASGRVSKATPEDADAPDGCVAPAVLAASQIAFTPA
                     SENGRPLAVVATVTYGFSEMRARVMDKKGHAPYCVTRRIPVSSPVEIVSVEGAKETEG
                     WRVIYE"
     gene            320..1138
                     /locus_tag="VGB76_22625"
     CDS             320..1138
                     /locus_tag="VGB76_22625"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY0101738.1"
                     /translation="MNEQVARREIVRVGQLMYERSYVVSSDGNVSVRLDDGRILATPT
                     QTNKGRMTEDSLALTDIEGKALNERKASSELAMHLLIYRERPEIKAVCHAHPPHGSAF
                     AVAGLAIDQPILSEVILTLGCVPLAEYGTPSTDELTAAMLPLVKHHNALLMANHGAVA
                     YGEDVWQAFDRLETLEHTAKIAILARALGGASNLPPDAIEKLINVREKAGYLDERARC
                     QSCGYLHETKITCATGDGAGKMGAARAATNGAGKISLTREDLVELLSQAVNLKN"
     gene            1460..2191
                     /locus_tag="VGB76_22630"
     CDS             1460..2191
                     /locus_tag="VGB76_22630"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEY0101739.1"
                     /translation="MKLLPRKFSVAPNHVGYLFRKNRFDVKLEPGIYRFFDPLEEIHV
                     VTLPITIKIQNIANQEVLTQDNIALRFSYFIEYRIGDPDKYITRFDVHTPFAGTFEAE
                     QLLHNLTQVYLRDLISKVLSEDLNERREELLNVVPETLRRDLSDYGLEIVRLMLRDVT
                     FPKAIQDLFARHLEAKIRAKADLENARTAVATARALKNAAELMKDDDNIRFFQLLETI
                     TKIADKGKHTFVFGDMQRGGLGMQK"
     gene            2240..2524
                     /gene="eutM"
                     /locus_tag="VGB76_22635"
     CDS             2240..2524
                     /gene="eutM"
                     /locus_tag="VGB76_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HEY0101740.1"
                     /translation="MAQEALGMVETKGLVAMIEAADAMVKAANVTLVGYEKIGAGFVT
                     AIVRGDVAAVKAATDAGAAAARRVGELVSVHVIPRPHGSVDDALPIGNNN"
     gene            2709..2981
                     /locus_tag="VGB76_22640"
     CDS             2709..2981
                     /locus_tag="VGB76_22640"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEY0101741.1"
                     /translation="MIIARILGTVVSTQKDERLRGKKLLIVRPLNLDGTEASGYTVAV
                     DTVGAGYHERVLVVAGSSARLAEGMKDSPVDAAIVGVIDTVDFIND"
     gene            3113..3403
                     /locus_tag="VGB76_22645"
     CDS             3113..3403
                     /locus_tag="VGB76_22645"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEY0101742.1"
                     /translation="MQLARVIGTVVATVKNDSLEGRKLLVIQSLDGRLRPQGAPLVAL
                     DAIGAGFGELVIWCRGKEASFPFRREDTPTDCTIIAIVDSERHVTPQGDAGK"
     gene            3400..3699
                     /locus_tag="VGB76_22650"
     CDS             3400..3699
                     /locus_tag="VGB76_22650"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEY0101743.1"
                     /translation="MILARVIGNVVATQKNERYRNARVMLCQPLSLEGEETGATLIAL
                     DSVDSGVGDMVLIVQEGWGASTAATKEAGAAIDSAIVGVVDYIDLLPEENKGERR"
     gene            3696..4205
                     /locus_tag="VGB76_22655"
     CDS             3696..4205
                     /locus_tag="VGB76_22655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101744.1"
                     /translation="MSAKEQDPVRSLAERIATRLAQDDVRESSASPERRDSSANVGDE
                     LAALRAGLSEIGQRLTQIESHVTHNTPRTSERAPQHDQHHDNAHRNTSTPARAPHHER
                     STAHTGSNPRHWLSGTYVPAVHPSEERFGIDEAVSELVEFFEREKVCNVEPGGKPCDH
                     CGMCSSRGF"
     gene            complement(4351..4983)
                     /locus_tag="VGB76_22660"
     CDS             complement(4351..4983)
                     /locus_tag="VGB76_22660"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY0101745.1"
                     /translation="MNKRRYFMLAFVVFVSMTASGCGYNTLQSKQQNVRAKWAGVENQ
                     LQRRADLVPNLVAAAQSAGVQEQEVFGQIAEARSRLLNATSAQPQGEGGDKSPEQKQA
                     IIEANNSFGGTIGRLLSLQENYPVLQSNQNFLKLQDELTGTENRLATARTDYTTAAQD
                     YNTTRGSFPTVLSAKLYGFKEEPYFKAEESARQAPRFDPNTMRRNQGDNK"
     gene            complement(5053..5796)
                     /locus_tag="VGB76_22665"
     CDS             complement(5053..5796)
                     /locus_tag="VGB76_22665"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0101746.1"
                     /translation="MANKVAQEAFNHLIGDLRATHGENLASVVLYGSAAAGDHIDESS
                     DYNLLIALTRITPEDLRQAQAPLREWRRLGHPLPVYFTTEELRDAADVFPIEFHQMQR
                     ARIVLYGADPFADLEVHDHNLRHQTEYELRSKLIQLRRLYIPASASIEKLTDLMGESL
                     TSFAVLFAPALMLHGIKEPPILKQECVRAAVRHFGLNGEPFERIFNLRTTGARPANDA
                     EAHELFTSYLAQIEIVTEAVDELRLGTGQ"
     gene            complement(5928..6785)
                     /locus_tag="VGB76_22670"
     CDS             complement(5928..6785)
                     /locus_tag="VGB76_22670"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY0101747.1"
                     /translation="MRTRRTFPALIVFAFLCLSGSATGAAYAQTATSPLPAPTGFVND
                     FANVIEPATEDRLENILTNLKTTGDIEFAVVTVPTTGEQDIFDYSLAVARGWGIGAKE
                     DESKGLLLIVAINDRKYFTQISRHLEGDLTDSLSGQIQRARLVPPFKQGDYSRGISDT
                     VETYVATLAEKRGFSLAGIDQSRAYRQPASQPRRTSNRRSGFSTCGTGLIILVVIIFL
                     LSSRGRGGGGSGCLSMLLLNSLLNSGGGRGSSGWGGGSFGGGGGGGGGFGGFGGGGSF
                     GGGGSGGSW"
     gene            complement(6926..7711)
                     /locus_tag="VGB76_22675"
     CDS             complement(6926..7711)
                     /locus_tag="VGB76_22675"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY0101748.1"
                     /translation="MIGFGGLLFFGALIALANVLGGLLLARPRTSLRDAHFLRYLIAL
                     GAGFMLAAIFLEILPEVVRLWGEGEEEISEAVLGAMTLLLTGYLLIHLFEHTIAPHFH
                     FGEETHTEAMLQPKAAYTAIAGLSIHTFFDGVSVAAAFLVNFKAGLLVFVAVLLHKVP
                     EGFTAASIMLASGRSPRRALAATLIIGAATLGGVLCVALLQSRVNVAVLYALPFSAGV
                     TLYVAASDLIPEVNHMERKNPLVSIVVFAGVALFYLLHKLIEG"
BASE COUNT         1789 a         2359 c         2425 g         1612 t
ORIGIN      
        1 gcggttcgct tgcgcgtgcg tttcgacgcg agcggtagag tctcgaaagc gactcccgaa
       61 gatgccgacg cgccggacgg gtgtgtcgcc cccgccgttt tggccgcctc acaaattgcc
      121 ttcaccccgg ccagcgaaaa cggcaggccg ctcgccgtcg tggcgacggt cacatacggc
      181 ttcagcgaaa tgcgcgcgcg agtgatggat aaaaaagggc atgcccctta ttgcgtgacg
      241 cgtcgaatcc cggttagttc cccggtcgag atcgtctcgg tcgaaggcgc gaaagagacg
      301 gagggttggc gcgtaattta tgaatgaaca agtcgcccgg cgcgagatcg tgcgcgtcgg
      361 ccagttgatg tatgaacgca gttacgtcgt ctcttcggac ggcaacgtga gcgtgcgtct
      421 ggacgacggg cgcatccttg ccacgccgac gcagaccaac aaggggcgca tgacggaaga
      481 ctcgctcgcc ctcaccgaca tcgaaggcaa ggctctgaac gaacgcaagg cttcgagcga
      541 actggcgatg cacctgctca tctatcgcga gcggcctgag atcaaagcgg tctgccatgc
      601 gcacccgccg cacggttcgg ccttcgccgt cgcggggctg gcgattgatc aaccgatcct
      661 gtcggaagtg atcctgactc tgggctgcgt gccgctcgcg gagtacggca cgccttcgac
      721 ggacgagttg accgcagcga tgctgccgct cgtgaagcat cacaacgccc tcttgatggc
      781 gaaccacggc gcggtcgctt acggcgaaga tgtctggcag gcgttcgacc gtctggagac
      841 tctggaacac acggccaaga tcgccatcct cgcacgcgct ctcggcggcg cgagcaatct
      901 tccgcccgac gcgattgaga agttgatcaa cgtgcgtgaa aaggccggtt acctcgacga
      961 gcgcgcgcgc tgccaatcgt gcggctacct gcacgagacg aagatcactt gcgcgaccgg
     1021 cgacggtgcg ggcaagatgg gtgcggcgcg cgcggcgaca aacggcgcag gtaagatttc
     1081 tctgacgcgc gaagatttgg tcgagttgtt gagtcaggcg gtgaacttga agaattagtt
     1141 gagcgcatcg tgacgtgtga aagaatagtc gcgcgcagaa gtttttgacg ataaccgcca
     1201 cctgtaacag ttcagaggct tgttcgattg gttacagggt attgcggaga acaagccaaa
     1261 cggggttcga ttccccactt tgcctggtaa gcaaactcga agctggttga aggagcgggg
     1321 acggcgcgag agcgcaagcc gtccgccgac ctgcaaaatc gaggataccg gcgcggctcg
     1381 aaattgagcc gcgactcaat cgattttcct cggcgtgcgg tgtctccgtc ttcttcgacc
     1441 ggttgaattt ttaaacgtga tgaagttatt gccgcgtaaa ttttctgtcg cccccaatca
     1501 cgtcggctac ttgtttcgca agaaccgctt cgacgtgaaa cttgagccgg gcatctatcg
     1561 tttcttcgac ccgctcgaag agatacacgt cgttacgctg ccgattacga tcaagattca
     1621 aaacatcgcc aatcaggaag tgctgacgca ggacaacatc gctctgcgct tctcctactt
     1681 catcgagtat cgcatcggcg acccggacaa atacattacg cgcttcgacg tgcacacccc
     1741 tttcgccgga actttcgagg ccgaacaact gctgcacaac ctgacgcaag tgtacctgcg
     1801 cgacctgatc tcaaaagtcc tgagcgaaga cttgaacgag cggcgcgaag aactcttaaa
     1861 cgtcgtgccg gaaacactgc ggcgcgatct gagcgactac gggttggaga tcgtgcgcct
     1921 gatgctgcgc gacgtgacct tcccgaaagc gattcaggat ttgttcgcgc gccatcttga
     1981 agctaagatt cgcgcgaaag ccgatctgga aaatgcgcgg acagccgtgg cgacggcgcg
     2041 tgcgctcaaa aacgcagccg agttgatgaa ggacgacgac aacatcaggt ttttccaact
     2101 gctcgaaacc atcacgaaga tcgcagacaa gggcaagcac accttcgtct tcggcgatat
     2161 gcagcgcggc gggctgggca tgcaaaaata ggttctcaaa agatcgcggc ggattaaatt
     2221 ctaaggagca acaaagctaa tggcacagga agcactggga atggttgaga cgaagggttt
     2281 ggtggcgatg atcgaagcgg cggacgcgat ggtgaaagcg gccaacgtca cgctcgtcgg
     2341 ttacgaaaag atcggcgcgg ggtttgtcac ggccatcgtg cgcggcgacg tggcggcggt
     2401 gaaggcggcg acggatgcgg gcgcggcagc cgcgcgtcgt gtcggcgaac tcgtcagcgt
     2461 ccacgtcatc ccacgcccgc acggcagcgt cgacgatgcg ctccccatcg gcaacaacaa
     2521 ttagaacagt gacaagtgac aggtaacggg tgacaagtca ggaagacgtg aattgtgaat
     2581 cgtcaatcgt gaatagaagg gatcggtctt aaatctattg acgattcacg attgacgatt
     2641 cacgatcact ggtcttgttc ctgctcgcca cttgtcactt gtcactcgtc acccggcact
     2701 gtcttcttat gattattgcg cgcatactcg gcacggtcgt ctccacgcag aaggatgagc
     2761 ggctgcgcgg gaagaaactc ttgatcgtgc gtccgctgaa tttggacggc acggaggcga
     2821 gcggctacac ggtcgccgtc gatacggtcg gggcgggcta ccacgagcgc gtgctggttg
     2881 tcgcagggtc gagcgcgcgt ctggccgaag gcatgaagga ctcgcccgta gacgccgcca
     2941 tcgtcggcgt gatcgacaca gtagacttca tcaacgattg aaagctgtta gcgattaacg
     3001 gttagctgtt ggccgtccgc cgttgtttaa ggtcgctcgt gcgcgtagaa agttgaccgc
     3061 cacggaaaga tttcaaagct gacagctaac agctaatcgt tagcaggctc ttatgcaact
     3121 tgcgcgtgtg atcgggacgg tcgtggcaac cgtgaagaat gattctctgg aggggcgcaa
     3181 gctcctcgtc attcaatcgc tcgacgggag gctcaggccg cagggcgcgc cgctcgtcgc
     3241 gctcgatgcg attggggcgg gcttcgggga gttggtcatc tggtgtcgcg ggaaggaggc
     3301 gagtttccct ttccgccgtg aagacacgcc gaccgattgc acgatcatcg ccatcgtcga
     3361 ttcggagaga cacgtgacac cgcaggggga cgccggaaaa tgattctcgc gcgcgtgatc
     3421 ggaaacgtgg tggcgacgca gaagaatgag cgttaccgta atgcgcgcgt catgttgtgc
     3481 cagcccttga gcctcgaagg cgaggagacg ggcgcgaccc tcatcgcgct cgattcggtt
     3541 gactccggcg tcggcgatat ggtgcttatc gtgcaggaag gctggggcgc atcgacggcg
     3601 gcgacgaaag aggcgggcgc ggccatcgat tcggccatcg tgggcgtggt ggattacata
     3661 gatttgctgc cggaagagaa caaaggagag aggcgttgag cgcgaaagag caagacccgg
     3721 ttcgttcatt ggccgagcgt atcgcaactc gtctggcaca ggacgacgtg cgggaaagtt
     3781 ccgcgtctcc cgaaaggcga gactcttccg caaacgtcgg cgatgaactc gccgcgcttc
     3841 gcgccggact ctcggagatc gggcagagac tcacccagat cgaatcgcac gtcacacata
     3901 acacgccgcg cacgtccgaa cgcgcacctc aacacgacca acaccacgac aacgcgcatc
     3961 gaaacacttc gacgcccgcg cgcgcgccgc accacgaacg ctcgacggcg cacaccggca
     4021 gcaacccgcg acactggttg agcggcacgt atgttcctgc ggtgcatccg agcgaggagc
     4081 gtttcggcat tgacgaggcc gtgtcggagt tggtggagtt cttcgagagg gagaaggtct
     4141 gcaacgtcga gccgggcggc aagccctgcg accattgcgg catgtgcagt tcgcgcgggt
     4201 tctaaagttc ggagcctaac ttcaaacgcg ctgcgcgttc cgcgtacgga agtcataaaa
     4261 tagagagggc gctttcaggt tgaaagcggc ctctctattt gtgtgtcgag ttttatctcg
     4321 cgggcgcgcg gcgaaagcgc gtccggtcta ttatttgttg tcgccttgat tgcgacgcat
     4381 cgtgttggga tcgaagcgcg gcgcttgacg cgcgctctct tcggccttga agtaaggctc
     4441 ttctttgaag ccgtagagtt tggcggagag cacggtcggg aaactgccgc gcgtcgtgtt
     4501 gtaatcctgc gcggcggtcg tgtaatcggt gcgcgccgtc gcgaggcggt tctcggtgcc
     4561 cgtcagttca tcctgcaact tcaggaagtt ttggttagac tgcaagaccg gataattctc
     4621 ttgcagcgaa agcagccgcc cgatggtgcc gccgaaacta ttgttggcct cgatgatcgc
     4681 ctgtttctgc tccggcgatt tgtcgccgcc ctcgccttgc ggctgcgcgc tcgtcgcgtt
     4741 caacaggcgc gaacgcgctt cggcgatctg tccgaaaact tcctgctcct gcacgcccgc
     4801 cgattgcgcc gccgcgacca gattcggaac caggtctgcg cgtcgctgta attgattctc
     4861 tacgcccgcc cacttggctc tcacattctg ctgcttcgat tgcagtgtat tgtagccaca
     4921 cccgctggcc gtcattgata caaagaccac gaaggccaac atgaaatatc ttcttttatt
     4981 cattactttc tccttattca atgcaagaaa tagacagccc ggaaatcaac cgtatgcaac
     5041 ttttgatccc gtctattgac cggtgcctaa tctcagttca tcaaccgcct cggtgacaat
     5101 ttcgatctgc gcaagatacg aggtgaacaa ctcgtgcgcc tccgcgtcgt tcgcaggacg
     5161 cgcgcccgtc gtgcgcaggt taaagatgcg ctcgaaaggt tcgccgttga gtccgaagtg
     5221 cctgaccgcc gcgcgcacgc attcctgttt caggatgggc ggctccttga ttccgtgcaa
     5281 catcagggcg ggcgcaaaca gcactgcgaa actcgtcaaa ctctcgccca tcaaatcggt
     5341 cagcttctcg attgaagccg acgcagggat gtagagcctt ctcagttgaa tcaacttgct
     5401 gcgcagttcg tactcggttt gatgacgcag gttgtgatcg tggacttcga gatccgcaaa
     5461 cggatcggct ccgtagagca cgatgcgcgc acgctgcatc tgatgaaact caatcggaaa
     5521 aacatccgcc gcatcgcgca actcttccgt cgtgaaatag accggaagcg ggtggccgag
     5581 tcgccgccac tcgcgcaaag gggcttgcgc ctgccgcaaa tcttcgggcg taatgcgcgt
     5641 taaggcgatc agcaaattgt agtcggaact ctcgtcgata tggtcgcccg ccgcagccga
     5701 gccgtacaac acgaccgatg cgaggttctc cccgtgcgtc gcgcgcaaat cgccgatcag
     5761 atgattgaaa gcctcttgcg ctaccttgtt agccatcagc actcctcgaa acgaaaagct
     5821 gtccgtcgcc cgtggtcaat tgtccgtcgt ttaagtggtt catacgttaa tcgctcaaga
     5881 ttcgatcaag caattaacaa cggacaactg aatacggaca aatcttctta ccaactgccg
     5941 cccgaaccgc cgccgccgaa actcccgccg ccgccaaagc cgccgaagcc gccgccacca
     6001 ccgcctccgc cgccaaaact cccgccgccc caaccgctcg aaccgcgacc gccgccgcta
     6061 ttcaaaagcg agttgagcaa cagcatgctc aggcaaccgc tcccgccacc gccgcgcccg
     6121 cgactcgaca ggagaaaaat gatgacgacc agaatgatca accctgtacc gcaggtggaa
     6181 aaaccgctcc gcctgttgct cgttcgtcgc ggctgcgacg cgggttgtcg ataagcgcgg
     6241 ctttgatcaa tgcctgccag actgaatccg cgcttttccg cgagcgtcgc cacgtaggtt
     6301 tcaaccgtat cggagatgcc gcgcgaatag tcgccctgct tgaaaggcgg cacgaggcgg
     6361 gcgcgctgaa tctgcccgga gaggctatcg gtcaagtcgc cttccagatg gcggctgatc
     6421 tgcgtgaagt acttccggtc gttgatcgcc acgatcaaca gcagcccctt gctctcgtcc
     6481 tctttcgcac cgatgcccca gccgcgcgcc acggccagag agtagtcgaa aatatcctgc
     6541 tcgcccgtgg tcggaaccgt gacgaccgca aactcgatgt ccccggtcgt ctttaagttc
     6601 gtcagaatgt tttccagacg atcctctgtc gccggttcga tgacgttggc gaaatcgttg
     6661 acgaagccgg tgggcgcggg cagaggcgaa gtcgcggtct gagcatacgc tgcgcccgtc
     6721 gcgctgcctg acaggcagag gaacgcaaaa actatgagag cgggaaatgt tcgtcgggtt
     6781 cgcatgggat gagttgataa tacaaggcgc aagccaaaag gtaaaaggca gaaacgaccg
     6841 tcccgcgcta cggcagagaa accggagcgt ttttcgctca cggcttcaca attctttaac
     6901 ttccgcacgc gcttttcgtc tccaatcatc cttcaatcag cttgtgcagc aggtaaaaga
     6961 gcgccacgcc cgcaaagacc acaatcgaaa cgagcggatt cttccgctcc atatgattga
     7021 cttccggaat gaggtcgctc gccgccacat aaagcgtcac gcccgccgaa aagggaagcg
     7081 cgtagagcac cgccacgttg acacgcgatt gcaacagcgc cacgcaaagc acgccgccga
     7141 gcgtcgccgc cccgatgatg agcgtggccg ccagcgcgcg tcgcggcgaa cgacccgacg
     7201 ccagcatgat tgacgcggcg gtaaaccctt cgggcacttt atgtaacagg acggcgacga
     7261 aaacgagcag tcccgccttg aagttcacca gaaaagccgc tgccacggaa accccgtcaa
     7321 aaaaggtgtg tatggacagt cctgcgatag ccgtgtaggc ggctttcggt tggagcatcg
     7381 cttcggtgtg cgtctcttct ccgaagtgga aatggggcgc gatggtatgt tcaaaaagat
     7441 ggatcagtag atagcctgtg agcagcagcg tcatcgctcc gaggacggcc tcgctgatct
     7501 cttcctctcc ctcgccccac aggcgcacaa cttcgggcaa aatctccaga aagatggctg
     7561 caagcatgaa tcccgcgccg agcgcgatca ggtagcgcag gaaatgtgcg tcacgcaggc
     7621 tggtgcgcgg gcgcgcgagc agcagcccgc ccagaacatt ggcgagcgcg atgagcgcgc
     7681 cgaaaaagag aagtccgccg aagccgatca tgcagaaatc gagaactcag tctgtagctg
     7741 tgtaaacctg atttatggcg ggcaaactat acaggatgcg acatgaaagt gacagttggg
     7801 agtttaagca gttctgcgct ccgcacacct ttttgacctg ctgcttaagc aattcggcct
     7861 ctcagagtcc ataatacaca ccaaatggtg cttaaaatta tctgttcgac attgtttttg
     7921 gtcttgacag tgtccggtaa tggagcgtag tattcgcggc gagtgggaga aagtggctag
     7981 aagtggactt ttgagagcat cttggcgcaa acggtttaag ttatacttta acttttaagc
     8041 aattctcgcc ccagccacac gccgaagccc tatgttaaga ggaaattaca cggcgcgtct
     8101 ggatgccaaa gggagactca aagtgccgac ccagttccgc cgccagatac aggaaaatca
     8161 cggcgcggaa gtctatgtaa cgagt
//
LOCUS       DASWNS010000654         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_207216, whole genome shotgun sequence.
ACCESSION   DASWNS010000654 DASWNS010000000
VERSION     DASWNS010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207216"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VGB76_22680"
     CDS             <1..396
                     /locus_tag="VGB76_22680"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0101749.1"
                     /translation="QRAKSKYHSGGLWSNTCCGHPRPGEATDAAARRRLREEMNFDCE
                     LGAAFEFLYRAEFANELIEHEYDHVFVGEFDGIVVPDEAEVENWRWMTLDELRRDLCV
                     RPDEYTYWLKAALDTDGWRRLEASLPAGR"
     gene            442..1479
                     /locus_tag="VGB76_22685"
     CDS             442..1479
                     /locus_tag="VGB76_22685"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEY0101750.1"
                     /translation="MLASNKTDQSHVVWGVEEWESLERRTREHALAQSSEAAAWKSIT
                     RQARGVMRAYTTSFFIVSRFLPAAKREEVEAVYAAVRYPDEIVDTFPLACAERLRLLD
                     EWSEGYEEGLAAGSIREAVRKGVPCFLAGFTSVVRERNIPPEHYRAFLKAMRHDAAPR
                     PFQTLADLIENYIYGSAIVVGYFLTHVYGASAQENFKRALESARALGIALQLTNFLRD
                     VGEDQRRGRVYLPLDMLRSEGIAAPAAAVVELDLNNPLQRYALAKVLKALASMAESYY
                     SDAEHELDAFSPDCQPAIRACINVYRRLNERIGQSPDGVLHRESVPVTEKFGVLPPSK
                     YWRIPLAYLRR"
     gene            1567..3075
                     /gene="crtI"
                     /locus_tag="VGB76_22690"
     CDS             1567..3075
                     /gene="crtI"
                     /locus_tag="VGB76_22690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEY0101751.1"
                     /translation="MKREREERVLIVGGGLGGLSAAIHLRLAGFAVSVYEANERVGGR
                     ANLIEGEGFRFDTGPSLLNYPWVFEKLFAAAGRNLYDYVKLLPVEPSVSFQWEDGTRF
                     TLSSDLRKFLEECERLEPGSRPRALEFMRDAEAKYRIAFEKMVHRNEDYVLPWLGALR
                     PAELWRTSIWRTLDGQLRRYFKSRHLREALGSYAMYLGGSPYTLPGMFTILSYGEMAY
                     GLWLPEGGVYGLVEGITKLASELGVEIHTSCRVQKIVTREGCIAGLELADGSFRQASL
                     VVSNVDAPTTDTELLAGASTRVTASLRRKAARTGMTPGVITFYWGVRGEVGHLGHHTI
                     FLPEDYRASFRDLLRRGRIPRGLPFYVSVPSATDAALAPAGDTAMFVLVPVPVISELP
                     GINWDETVGEVRAQVLGTLARHDIEFTEDRLVFEEVYTPVEWQRRFGLYDGSAFGAAH
                     NLLQLGPLRRKNFSAEINGLYYAGASTTPGTGMPLVVLGGQQVAERIAGHVR"
     gene            3065..3931
                     /locus_tag="VGB76_22695"
     CDS             3065..3931
                     /locus_tag="VGB76_22695"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0101752.1"
                     /translation="MYVKRYGSGAQTYFGLHGWSGDHRTFAPLAAHLPARASLYSADL
                     PGCGASPARRALTLDSITNEIVEAIDKAAPARGRATLIGNCSGAILGLLVAQRIESKL
                     ARLVLVDPFAYWPWYFRVFVAGSFGRHAYRTTFATSFGRWLTNQSLRGHRTAETDLTN
                     SFGRIDHEVAQRYLALLAEVGGIEQFAGLRRVPIDIVYGERTFGAVKRSVAMWQGIWP
                     QARAFVLKGAGHLPLHEATVALSEIVFTQHEGAKSLSAHGAATDTATDAATDAATGAT
                     ATAQATESEAGV"
BASE COUNT          745 a         1256 c         1320 g          728 t
ORIGIN      
        1 cagagggcga aatccaaata ccactccggc ggcctctggt cgaatacctg ttgcggccac
       61 ccgcgccccg gcgaggcgac cgacgcggct gcgcgccgtc gcctgcgcga agagatgaac
      121 ttcgattgcg aactcggcgc ggccttcgag tttctctacc gcgccgagtt tgccaacgag
      181 ttgatcgagc acgaatacga tcacgtcttc gtgggagagt ttgacggcat cgtcgtgccg
      241 gacgaagcgg aagtggagaa ttggagatgg atgacgctcg acgaactgcg ccgcgacctg
      301 tgcgtgcgac cggacgagta cacctactgg ctcaaggccg cgctcgacac ggacggctgg
      361 cgtcgcctcg aagcgtctct cccggcaggg cgatagcccg cgcgcagagt tcagggcacg
      421 cgccgccgcg caaagatttt tatgcttgcg agtaacaaga cagatcagag ccacgtcgtc
      481 tggggcgtgg aagagtggga gtcgcttgag cggcggacgc gcgaacatgc gctggcgcag
      541 agttccgaag cggcggcgtg gaagagcatc acacggcagg cgcgcggggt gatgcgcgcc
      601 tacacgacga gtttctttat cgtgagccgc ttcctgcccg ccgccaagcg tgaggaagtg
      661 gaagccgtct atgccgccgt gcgttacccg gacgagatcg tagacacgtt tccgctggct
      721 tgcgcggagc gtctgcgcct gctcgacgag tggagcgagg gttacgaaga gggtctggct
      781 gcggggtcaa tcagggaggc tgtgcgcaaa ggcgtgccgt gctttctggc cggttttacc
      841 agcgtcgtgc gcgagcgcaa catcccgccc gaacactatc gcgcttttct caaagcgatg
      901 cggcacgatg ccgcgccgcg cccgttccag acgctcgcag acctgattga gaattacatc
      961 tacggcagcg caatcgtcgt cggctacttt ctcacgcacg tctatggcgc gagcgcgcag
     1021 gaaaatttca agcgcgcact tgagagcgca cgcgcgctcg gcatcgcgtt gcaactgaca
     1081 aatttcctgc gcgacgtagg cgaagaccag agacgcgggc gcgtctacct gccgctcgac
     1141 atgctgcggt ctgagggcat cgccgcccct gccgccgccg tcgtcgagtt ggacttgaac
     1201 aacccgctcc aacgctacgc gctcgccaaa gtgctcaagg ctttggcgtc aatggccgag
     1261 tcatactact cggatgcaga gcacgaactg gacgccttct cgccggattg ccagcccgcg
     1321 atacgcgcct gcatcaacgt ctatcgtcgt ctgaacgaac gcatcgggca aagcccggac
     1381 ggcgtgctgc atcgtgagag cgtgcccgtg accgagaagt tcggcgtcct gccgccgagc
     1441 aaatactggc gcatcccgct cgcctacctg cgccgctgag tcgagccttc ggagagtcgc
     1501 cgtagtgcgc aggggcgaga aaggcgtggg cgaaaagtct gcgggtgagt tgaactgatc
     1561 ggacggatga agagagagag agaagagcgt gtcttgatcg tcggcggcgg gctgggaggc
     1621 ttgagcgcgg cgatacacct gcgcctggcg ggcttcgcgg tgagcgtcta cgaggcgaac
     1681 gagcgcgtgg gcggtcgcgc caacttgatc gagggcgagg ggttccgttt cgacacgggg
     1741 ccttctctgc tcaactaccc gtgggtcttc gagaaattgt ttgccgccgc cgggcgcaac
     1801 ctgtacgact acgtgaagct gctgcccgtc gaaccgtccg tcagtttcca gtgggaggac
     1861 ggcacgcgtt ttacgctctc aagcgacctc agaaagtttc tggaagagtg tgagcggctc
     1921 gaacccggct cgcgcccgcg tgcgctcgaa ttcatgcgcg acgcggaggc gaaataccgg
     1981 atcgctttcg agaagatggt gcaccgcaac gaggactacg tgctgccctg gctcggcgcg
     2041 cttcgcccgg cggaactctg gcgcacgagc atttggcgca cgctcgacgg ccagctccgt
     2101 cgctacttca aaagccggca cctgcgcgaa gccctcggct cctatgcgat gtatctcggc
     2161 ggttcgccct atacgctgcc cggcatgttc accattctct cttacggcga gatggcttac
     2221 ggactctggc tgcccgaagg cggcgtctat ggtctggttg aagggatcac gaaactggcc
     2281 tccgaactcg gagtcgaaat acacacgagt tgccgcgtgc agaagatcgt cacgcgcgaa
     2341 gggtgcatcg ccggtctgga actcgccgac ggcagctttc gccaagcctc tctcgtcgtc
     2401 tctaacgttg acgcgccgac aaccgacacg gagttgctcg ccggagcctc gacacgcgtg
     2461 accgccagcc tcaggcgcaa agccgcgcgg acgggaatga cgcccggcgt cataactttt
     2521 tactggggcg tgcgcggcga ggtcggccat ctcggccacc acacgatctt tctgccggaa
     2581 gactatcgcg cctccttccg agacttgctg cggcgcgggc gtatcccgcg cggcctccct
     2641 ttttacgtga gcgtgccttc cgccaccgac gccgcactcg cgcccgcagg cgatacagcg
     2701 atgttcgtgc tcgtgcccgt gcccgtcatc agcgagttgc cggggataaa ttgggatgag
     2761 actgtcggcg aagtgcgcgc gcaggtctta gggacgctcg cgcggcacga catcgagttc
     2821 acggaggatc gactcgtctt tgaagaggtc tatacgcccg tcgaatggca gcgacgtttc
     2881 ggcctctacg acggttcggc gttcggcgcg gcgcacaatc ttttgcaact cggacccctg
     2941 cgccggaaaa atttttccgc agagatcaac ggactctatt acgcgggcgc gagcacgacg
     3001 ccgggcacgg gaatgccgtt ggtcgtgctc ggcgggcagc aggtcgcgga gaggatcgcc
     3061 ggtcatgtac gttaagcgtt acggttcggg cgcacagacc tatttcggcc tgcacggttg
     3121 gagcggcgac caccggacgt tcgcgccgct cgccgcgcac ctgcccgcgc gtgcgtcgct
     3181 ctacagcgcg gacttgcccg gctgcggcgc atctcccgcg cgtagagcct taacgctcga
     3241 ctcgatcacg aacgagattg tcgaagccat agacaaggcc gcgccagcgc gcgggcgcgc
     3301 cactttgatc ggcaattgca gcggcgcgat tctcggcctg ctcgtcgcgc agagaatcga
     3361 atcgaagctc gcgcggctcg tcctcgtcga tcctttcgcc tactggcctt ggtatttcag
     3421 agtcttcgtc gcggggagtt tcgggcggca cgcttaccgt acgacgttcg ccacttcgtt
     3481 cggacgctgg ctgaccaacc agtcgctgcg cggccatcgc accgccgaaa ccgatctcac
     3541 aaattcgttt ggccggatcg atcacgaagt agcgcaacgt tatctcgcgt tgctcgcgga
     3601 agtcggaggc atcgagcagt tcgcgggatt gcggcgcgtg ccgattgaca ttgtctacgg
     3661 cgaacggacg ttcggcgcgg tgaagagatc ggttgcgatg tggcagggca tctggccgca
     3721 ggcgcgcgct tttgtgctca aaggcgcggg tcacctgccg ctccacgaag cgaccgtggc
     3781 cttgagcgag atcgtcttca cgcagcacga gggggcgaaa agtctttccg cgcacggagc
     3841 cgcaacagac acagcgacag atgcggcgac agatgcggcg acaggagcga cggcgacagc
     3901 acaggcaaca gaaagcgagg cgggagtttg accgaacgac taacccattt catcgcacaa
     3961 cacggcgcgg agatcgagtt cgcactgcgt atgaacctgc ccacgagttc gcaggcgggc
     4021 gcggagcgtc tgaacgacgc actctcata
//
LOCUS       DASWNS010000655         8130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_259018, whole genome shotgun sequence.
ACCESSION   DASWNS010000655 DASWNS010000000
VERSION     DASWNS010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259018"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VGB76_22700"
     CDS             <1..850
                     /locus_tag="VGB76_22700"
                     /inference="COORDINATES: protein motif:HMM:NF016165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HEY0101753.1"
                     /translation="HAARDVRDPKTGKSVYQAWQDRVNESRPAAELTDASAGTDAPIA
                     EARISALGSGSDYTPFLQHIGVSSLDMGFGGDYGVYHSTYDSFNWMSKFGDPDFTYHV
                     AAAQIWGTIALRLANSRALPFDYADYATELRDFINETQKTAARRKLADDFDVRALLDA
                     VKDLSDEAARTQARRNQLLEEIESRLRVGDREPRASMLLKRSNDSLIAAERALTDERG
                     LRGRPWYRHQIYAPGLYTGYAAQPLPDLRQAIDDRNTTNTREAALRITEAIRRAVGVL
                     REGRGN"
     gene            complement(968..1558)
                     /locus_tag="VGB76_22705"
     CDS             complement(968..1558)
                     /locus_tag="VGB76_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101754.1"
                     /translation="MLKNFAPRRRRLSASLLYALVVAVSLLQPAHTNAAPGQRREHLS
                     EQEVEMVRDTQELDRRSDLFIKIAERRLLILTGAPAAPRPSAKDLEKWGEVPKGTRAA
                     LFNDLARIFDEAINNVDDVASRKPDSELLPKAVRKLAEGATRFLPQLAALRQSATAEG
                     EREALEQAIDNLQEIIDAAKRLPEETTEKKSEKKKN"
     gene            complement(1579..2223)
                     /locus_tag="VGB76_22710"
     CDS             complement(1579..2223)
                     /locus_tag="VGB76_22710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101755.1"
                     /translation="MKRSIVYRRRVVSAVVLFAACAAWLSAEARAQDLILPPQPAPPP
                     MVYIPESARTQLLSARDEKARTRLSIELVNARLASAEQHTALKQFNAATAELGIYQAL
                     FEDALQYLHRSGGNNARDLYKRLEQTLHRHAARIESIRRTTPSEFSGNIRALIQLVRD
                     LRTEALEAFYGDTVLSANVGDRTNFVEPERRDTLPETTPARVAFTRESEPEINS"
     gene            complement(2265..3530)
                     /locus_tag="VGB76_22715"
     CDS             complement(2265..3530)
                     /locus_tag="VGB76_22715"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101756.1"
                     /translation="MPTVFPLRFNTRSVVLRALLVLLALSLSLHAQEADDVADGEDDP
                     VKIFNRAQDAHARGDLARALALYTEAIKVRPEFPEAEYQKAAAHVALKQTAEAEKSLR
                     RAIELRADWSLPQTALGLLLVRAGDDAKAEPYLRRALQLDAKDTNALVALASLRLRAG
                     AKEEAVKLIVRATADEAATAATWATRARIERSAGDKTAAAASIERALRLDPNNAAALE
                     ERTELAADTDDYERAIADIETALRAAPESAQLRARLANYHALAGEQQRTIDPNKSLAH
                     YRRAAELEPASIGHATGYASALVQARRFAEAAALLKRILASAPDNYAAHANLATALDG
                     LKQYPEALVEYQWLNRARPELAVTYFLLARAYDLTGDLELALATYETFLAKADAAQNN
                     LEIEKVNLRLPNLRRQIKNGQGTKRRKNQ"
     gene            3845..4729
                     /gene="larE"
                     /locus_tag="VGB76_22720"
     CDS             3845..4729
                     /gene="larE"
                     /locus_tag="VGB76_22720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEY0101757.1"
                     /translation="MAVSSSVVEEISAEAEAAVSHEAAEKELALRALFREMRSVLVAF
                     SGGVDSSYVAYVATGELGGRALCVTGESSSLAAHQRTQASEIAARFNFRHETINTEEM
                     SDPRYTANAGDRCYFCKTELYSKLAPLAAERELSCVVDGSTTDDLGDYRPGRAAAQEH
                     GVRSPLVEVRMSKSEVRELSRRAGLPTWDAPASPCLSSRIAYGTTVTIKRLREVETGE
                     EIMRSLGFREFRVRHHDELVRLEIAPAELERALRREVTDELARRFRALGFRYVTLDLH
                     GYRTGAMNEVLKAVTSHE"
     gene            4800..6086
                     /locus_tag="VGB76_22725"
     CDS             4800..6086
                     /locus_tag="VGB76_22725"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY0101758.1"
                     /translation="MAATTSVEDALPEAKEANPFESMMSRFDRAAKLLDLNPDLYAVM
                     RVPNRELKVYIPVRMDSGRIQVFEGFRVQHNFARGPAKGGIRYAPDVTIEEVRALAAW
                     MTWKCAVVNVPFGGGKGGVICDPWQMSQGELERLTRRYASELIDIIGPEKDVPAPDMN
                     TNEQTMAWIMDTYSMHARHTVNAVVTGKPVALGGSQGRREATGRGVLFVVNEAMKKFK
                     MTPPTTRVVVQGSGNVGGIGAQLMHEAGYKVMAISDMHGGIYNPAGIDIPAALEHLRA
                     TRSFEEYPDAERVSNAELLEIECDVLVPAATENQITSQNAERINCKVLAEGANGPTTA
                     AADEILQRKGIFVIPDILANAGGVTVSYFEWVQDRMGYFWKEDVVNERLQDVMVASFN
                     ELVKFAEKHNVDTRTAAYMLAIDRVAYDTRMRGIYA"
     gene            6621..>8130
                     /locus_tag="VGB76_22730"
     CDS             6621..>8130
                     /locus_tag="VGB76_22730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101759.1"
                     /translation="MRLARRAQVLALTLLVLCAAVSAQTLRPADDPRNTAPTVGTGGP
                     VGGPTGLFTIYDGQTLRKGEFTFSIAYSNFDRDPGDVDITEVPLSFQIGLSDHLELFF
                     NTVGYRGVKVNSPQNLSSFYLPNTAIFTQRLQANGVLLPNTLQSGGAIVLAPINNVVI
                     GQQALFRPVGNQPFVQFPFVGGSAGNFNNGFAGFNPRFVFTLGGPTGPNASGSNFGAA
                     ANFPGIGSIFGSILPGVVLTQRTIPATLTTSAQVVPDTFTVAPTYLPEAPFISRLYGE
                     SSFSTFSIGAKWRITGPNNPFGFGFIPFYRFYGDTADDRSGFNQLQRGASPGGSLGDF
                     GLVMFADGRLSRSVNISANLGYILNSNPRSELLGDAALLDRPDEFIAGLGVDFPVNQH
                     FQPIMEVKSVQYVGGRTPNAFQNNPVDFLAGVRIFPRRWMGFSAAYRAHMNPQGDRIF
                     NDRSPSEFPRGFRQSQDPHGYIFQFWAGRRNERAPTIVPNNPPTTAVSASPSR"
BASE COUNT         1554 a         2449 c         2520 g         1607 t
ORIGIN      
        1 ccacgccgcg cgcgacgtgc gcgatccgaa gaccggcaag tctgtctatc aggcttggca
       61 ggatcgtgtg aacgaatcgc gccccgcagc agagttgacc gatgcgagcg cgggcaccga
      121 cgcgcccatc gccgaagcac gcatcagcgc gctcggttcc ggttcggact acacgccctt
      181 cctacagcac atcggcgtct cttcgctcga catgggcttc ggcggcgact acggcgtcta
      241 tcactcgacc tacgattcct tcaactggat gtcgaaattc ggcgaccccg atttcaccta
      301 ccacgtcgcc gccgcacaaa tctggggcac gattgccctc cgcctcgcca actcgcgcgc
      361 cctccccttc gactacgccg actacgccac cgaactccgc gactttatca acgagacgca
      421 gaagaccgcc gcgcggcgca aactcgcaga cgacttcgac gtgagagctt tgctcgatgc
      481 cgtcaaagat ttaagcgatg aagcggcacg cacgcaggct cgccggaatc aattgctgga
      541 agagatcgaa tcgcgcctgc gggtcggcga cagggagccg cgcgcctcga tgctgctcaa
      601 gcgcagcaac gatagtctca tcgccgccga acgcgctttg acggacgaac gcggcctgcg
      661 cggtcgcccc tggtaccggc atcaaatcta cgcgcccgga ctctacacag gctacgccgc
      721 gcaaccactg cccgatctcc gtcaggcgat cgacgaccgc aacaccacga acacacgcga
      781 agccgccctc cgcatcacgg aagcgattcg ccgcgccgtc ggcgtgttga gagagggaag
      841 agggaattag acgggcgaag caggcgaagc aaaagttaga agcaaaagtt aaaggccgcc
      901 ggatcacttt atgttccggc ggccttcgac gttgtgtgac gcatgcctca cgatgacgac
      961 gcgcgtttca gtttttcttc ttctcgcttt tcttctcggt cgtctcttcg ggcagacgct
     1021 tcgcggcgtc gatgatctcc tgtaggttgt caatcgcctg ttcgagggct tcgcgttcgc
     1081 cttcggcggt cgcgctctgg cgcagggcgg cgagttgggg gaggaaacgc gtcgcgcctt
     1141 cagccagttt cctcacggct ttcgggagga gttcgctgtc gggcttgcgc gaggccacgt
     1201 cgtccacgtt gttgatcgct tcgtcgaaga tgcgcgcgag atcgttgaag agcgcggcgc
     1261 gcgtgccctt cggaacttcg ccccacttct ccaaatcttt ggcggaaggc ctcggcgcgg
     1321 cgggcgcacc cgtgaggatg agcagccgac gctcggcgat cttgatgaag aggtcggagc
     1381 gtcgatcaag ttcctgcgtg tcgcgcacca tttcgacttc ctgttccgac aggtgttcac
     1441 gccgctgacc cggcgcggcg tttgtgtgcg cgggttggag cagcgagacg gcgacgacga
     1501 gcgcgtagag cagtgacgcg ctcaggcgtc tgcgacgagg ggcgaaattt ttcaacatgt
     1561 gtttactcga cttaagagtt aagagttgat ctccggctcg ctttcgcgtg tgaaggcgac
     1621 gcgagcagga gtcgtctccg gcaaagtatc tcttcgttcc ggctcgacaa agttcgtccg
     1681 gtcgccgacg ttcgcgctga ggacggtgtc gccgtagaag gcttcgagag cttcggtgcg
     1741 caggtcgcgc actaactgga tcaacgcgcg aatgttgcct gagaactcgc tcggcgtggt
     1801 gcggcgaata gactcgatgc gcgccgcgtg cctgtgcaat gtctgttcga gacgtttata
     1861 gagatcgcgc gcattgttcc cgcccgaacg gtgtagatac tggagcgcgt cttcaaacaa
     1921 agcctggtag atgccgagtt cggcggtggc ggcgttgaat tgtttgagcg ccgtgtgctg
     1981 ctccgcgctt gcgaggcgcg cgttgacgag ttcgatgctg agacgcgtgc gcgccttctc
     2041 atcacgcgcc gacaataact gcgtgcgcgc gctttccggg atgtagacca tcggcggcgg
     2101 cgcgggctgc ggcggcaaga tcaaatcctg cgcacgcgct tccgcagaca gccacgccgc
     2161 acacgccgca aacagcacaa ccgccgacac tacacgcctg cgatagacaa ttgaacgctt
     2221 catggaaact tcctgcgaca aactctctcc cccggttctg attgttactg atttttcctg
     2281 cgcttcgtgc cctgcccgtt cttgatctgc ctgcgcagat tcggcaggcg caagttgacc
     2341 ttctctatct ccagattgtt ttgcgcggcg tcggccttcg ccagaaaagt ttcgtaagtg
     2401 gcgagcgcga gttcgaggtc gcccgtcaga tcgtaagcgc gcgcgagaag aaaataggtg
     2461 acggcgagtt cggggcgcgc acggttgagc cactggtatt cgacgagtgc ctccgggtac
     2521 tgcttcaaac cgtcgagagc cgtcgccaga ttggcgtgcg ccgcgtagtt gtccggcgcg
     2581 ctggcaagga tgcgcttcag gagcgcggcg gcttcggcga agcgacgcgc ctgcaccagc
     2641 gcagacgcgt agccggtggc atgaccgatg ctcgccggtt cgagttcggc ggcgcggcga
     2701 taatgtgcca gcgacttgtt cggatcaatc gtgcgctgct gttcgcccgc gagcgcgtga
     2761 tagttggcga gtcgtgcgcg caactgcgca gactcgggag cggcgcgcag ggctgtctcg
     2821 atatctgcga tggcgcgctc gtagtcgtcc gtgtcggcgg cgagttcggt gcgttcttcg
     2881 agcgcggcgg cgttgttcgg atcgagacgg agcgcgcgtt cgatgctcgc ggcagcggcg
     2941 gtcttgtctc cggcggagcg ttctatacgg gcgcgcgtcg cccacgtcgc ggcggttgct
     3001 gcttcgtctg cggtcgcgcg cacgatcagt ttgacggcct cttctttcgc tcccgcgcgc
     3061 aggcgcaagg aggcgagcgc gacgagcgcg ttcgtgtctt tggcgtcgag ttggagggcg
     3121 cggcgcaggt aaggctcggc tttggcgtcg tcgcccgcgc gcactaaaag gagtccgagc
     3181 gcggtttgcg ggagcgacca atcggcgcgc agttcgatgg cgcggcgcag ggatttctcc
     3241 gcctccgccg tctgcttgag cgcgacgtgc gccgccgcct tttgatattc cgcttcgggg
     3301 aattcggggc gcaccttgat cgcttccgtg tagagtgcga gggcgcgtgc gaggtcgcct
     3361 ctggcgtgtg cgtcctgcgc gcggttgaag atcttcaccg gatcgtcttc gccgtcggcc
     3421 acgtcatccg cctcttgcgc gtgcagcgag agggagagtg cgagcaggac gagaagcgcg
     3481 cggaggacga cgctgcgcgt gttgaagcgt agaggaaaaa cagtgggcat cgaaactgcg
     3541 tgtgctttcg ttcgaatgtg aaaattgttt gccgaaactt gaattaaaag aggtgtcggc
     3601 gacgccgaaa ggctgttctg ccgcgcgagc ataacacgca ctttggagac gcgtccacga
     3661 cgaccggcga cgaactctcg cgctttgtat ggatgatggg ttgcgtcctt caagatgcct
     3721 gcgataacat gcccgctatc ctgattttca aactcaaact tgaggtcgca cgcgtgggcg
     3781 tcgaggcttc acacaggtca aacggccttc tcagaacgga caagggtttc ggagcggagt
     3841 aaaaatggct gtgagcagtt ccgtagttga agaaatttcg gcggaagcgg aggcggcagt
     3901 ttcgcacgaa gcggcggaga aggagcttgc cctgcgcgct ctcttccgcg agatgagatc
     3961 ggtgctcgtg gccttctccg gcggcgtaga cagttcctac gtggcctacg tcgcaacggg
     4021 cgaactcggt ggacgcgccc tgtgcgtgac gggcgaatcg tcgagcctcg ccgcgcacca
     4081 gcgcacgcaa gcctccgaga tcgccgcgcg cttcaatttc aggcacgaga cgattaacac
     4141 cgaagagatg tccgacccac gctacacggc gaacgcgggc gaccgctgct atttttgtaa
     4201 gaccgaactc tactccaaac tcgcgccgct cgccgccgaa agggaactga gttgcgtcgt
     4261 tgacggttcg acgacggacg acctcggcga ttatcgtccc ggtcgcgcgg cggctcagga
     4321 acacggcgtg cgcagccccc tcgtcgaagt ccgaatgagc aaaagtgaag tccgcgaact
     4381 cagccgtcgt gccgggctgc cgacgtggga cgcacccgcc agtccctgtc tctcttcgcg
     4441 catcgcttac gggacgaccg tgaccatcaa gcgtctacgc gaggtggaga cgggcgaaga
     4501 gatcatgcgc tcgctcggtt ttcgcgagtt tcgcgtcagg catcacgacg aattggtgcg
     4561 cctcgaaatc gccccggcgg agcttgagcg cgcgctccga cgcgaagtca cggacgaact
     4621 cgcgcgccgt ttccgcgccc tcggctttcg ctacgtgacg ctcgacctgc acggctatcg
     4681 cacgggcgcg atgaacgaag tcttgaaagc agtgacgagt cacgagtaac aagtgacaag
     4741 ttttagaact tgttttaact catcactcat cactcgtcac ttgtcactag gagatcaaaa
     4801 tggctgctac tacgtcggtt gaagacgctc tgccggaagc gaaagaggct aacccgttcg
     4861 agtctatgat gtcgcgtttt gaccgcgcgg cgaagttgct cgacctcaat cccgatctct
     4921 acgccgtgat gcgcgtgccg aatcgggaac tgaaagttta catcccggtg cgcatggact
     4981 ccgggcgcat acaggttttt gaagggtttc gcgtgcagca caattttgca cgcggcccgg
     5041 cgaaaggcgg catccgttat gcgcctgacg tgacaatcga ggaagtgcgc gcgctcgccg
     5101 cgtggatgac ctggaagtgc gcggtcgtta acgttccgtt cggcggcggg aaaggcggcg
     5161 tcatctgcga cccgtggcag atgtcgcagg gcgaacttga gcggctcacg cgtcgttacg
     5221 cctccgaact gatcgacatc atcgggcctg agaaagacgt gcccgcgccc gacatgaata
     5281 cgaacgagca gacgatggcg tggatcatgg atacctactc gatgcacgcg cgccacacgg
     5341 tcaatgccgt cgtcaccggc aagcccgtcg cgctcggcgg ctcgcagggc aggcgtgagg
     5401 ctacggggcg gggcgtcctg ttcgtcgtca acgaggcgat gaagaagttc aagatgacgc
     5461 cgcccacgac gcgcgtggtc gtgcaaggct caggcaacgt cggcggcatc ggcgcgcagt
     5521 tgatgcacga ggccggttac aaagtgatgg cgatctccga catgcacgga ggcatctaca
     5581 accccgccgg aatcgacatc cccgccgctc tggagcatct gcgtgcgacg cgctctttcg
     5641 aggaataccc ggacgccgaa cgcgtgtcga acgccgaact gctcgaaatc gagtgcgacg
     5701 tgctcgtgcc cgccgcgacg gagaaccaga tcacttcgca aaacgccgag cggattaact
     5761 gcaaggtgct ggccgaaggc gcgaacggcc cgacgactgc cgccgccgac gaaattctcc
     5821 agcgcaaagg catctttgtg attccagata ttttagccaa cgcgggcggc gtcaccgtct
     5881 cctacttcga atgggtgcaa gaccggatgg gctatttctg gaaggaagat gtcgtcaacg
     5941 agcgtttgca ggacgtgatg gtcgccagtt tcaacgaact cgtgaagttt gccgagaagc
     6001 ataatgtgga cacgcgcacg gcggcttaca tgctcgccat tgaccgcgtg gcctacgata
     6061 cgcgcatgcg cggcatctac gcgtgacaaa aatgtgtagg gatttttcat gctaagtcgt
     6121 gaaaaagtct tgatttccgc gccaatgtag agtatggtct gccggaaatc gttatgtagg
     6181 agcagagtcg gaaagtgtaa cttaacgata aagttccagc atttcgagtt cgcagcgttg
     6241 acagtggcgt gactgttttg tataagcagt accgcattac agttaatcag gctaaaagaa
     6301 gaatacagtt gagaggcgca aagaggcaga ttttaagcgc ccgcgaacac agaatacgtg
     6361 ttcggctcct ctcgctgctc cgctccagct acaaggcgtt tcgttcgagg gcggtagcag
     6421 ttttacattg actctgttgt gtacgcttgt agttggagcg tctgcccacc gaaagttcaa
     6481 gggaacgtgt tggattgtca cgaggaaagc aattgataca gacagttgta gccgcagaca
     6541 aaagcgcgaa cctttgggct agggataggc agtcgcccct tcccgcaaaa ttacattact
     6601 catttcgcag gaggaatgcg atgagactcg cgagaagagc gcaagtattg gcgctcaccc
     6661 tgttggtgct gtgtgccgca gtatcagctc aaacactaag gccggcggat gatccgagaa
     6721 acaccgctcc gaccgtaggc acaggtggcc ccgttggcgg ccccaccgga ctcttcacca
     6781 tttacgacgg ccagacgctg cgtaaaggtg aattcacatt cagcattgca tacagcaact
     6841 ttgaccgcga cccgggcgac gtggacatca cggaagtccc gctcagtttc cagatcggcc
     6901 tctcagacca cctggaactg ttcttcaaca cggttggcta tcgcggcgtc aaagtcaaca
     6961 gcccgcagaa cctgtcgagc ttctatttgc ccaacactgc gatctttacg cagcgtctgc
     7021 aagcgaatgg agtgctactg cccaacacgt tgcagagcgg cggagccatc gtgctcgccc
     7081 cgatcaacaa cgtggtgatc ggccagcagg ctctgttccg ccccgtcggc aaccagccct
     7141 tcgtgcagtt cccgttcgtc ggcggttcgg caggcaactt caataacggc ttcgctgggt
     7201 tcaacccgcg cttcgtgttc acgctcggcg ggccgaccgg cccgaacgcc agcggcagca
     7261 acttcggcgc ggcggcgaac ttccccggca tcggctcgat cttcggcagc atcctgcccg
     7321 gcgtcgtgct gacgcagaga acaataccgg cgaccttgac gaccagcgca caggtcgtgc
     7381 cggatacgtt cacggtcgcg ccgacttact tgcctgaggc gccgttcatc agccgtctct
     7441 acggcgaatc gtcgttcagc acgttcagca tcggcgcgaa gtggcgcatc accggcccga
     7501 acaatccttt cggcttcggt ttcatcccgt tctatcgctt ctacggcgac acggctgacg
     7561 accgcagcgg cttcaaccag ttgcagcgcg gcgcaagccc cggcggcagc ctcggcgact
     7621 tcggcttggt catgttcgcc gatggtcgtc tgagccgcag cgtcaacatc tcggccaacc
     7681 tcggctacat cctgaacagc aacccgcgca gcgagttgtt gggtgatgcg gccttgctcg
     7741 accgtcctga tgaatttatc gcgggcttgg gcgtagactt cccggtcaac caacacttcc
     7801 agccgatcat ggaagtgaag tctgtgcagt acgtgggcgg cagaacgccg aacgctttcc
     7861 agaacaaccc ggttgatttt ctggctggcg tccgcatctt cccgcgtcgc tggatgggct
     7921 tcagcgcggc ttatcgcgcg cacatgaacc cgcagggcga ccgtatcttc aacgaccgct
     7981 cgccttcgga attcccgcgc ggcttccgtc aatcgcaaga cccgcacggc tacatcttcc
     8041 agttttgggc tggtcgccgc aacgagcgcg ctcccaccat cgtgccgaac aatccgccga
     8101 cgacggctgt ttcggcaagc ccgtcgagag
//
LOCUS       DASWNS010000656         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_102923, whole genome shotgun sequence.
ACCESSION   DASWNS010000656 DASWNS010000000
VERSION     DASWNS010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102923"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VGB76_22735"
     CDS             <1..1130
                     /locus_tag="VGB76_22735"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0101760.1"
                     /translation="KVFVITAESRDAQARFNAKDRGIGLARDDARHAWRIYSLDRNTG
                     KINWERTAYEGVPRAKRHVKATQANSTPATDGKTIVALMGSEGLYAYDFAGKLLWKQD
                     LGVLNPGLAGDPTSEWGHASSPVIYRGLVIVQCDKHRDSFLAAYHLKDGKLAWRVERD
                     ELPSWSPPAIYEGRTRAELVTNASNYIRGYDPLTGKELWRFANGATQVQMPAPVVAND
                     MIIVTGGYPGNRPVHAFRPGASGDISLKEGEESTPALVYRLPRGSSYTGTPLVYRDLM
                     YISGDNGVLRVFEAKTGKQLYEQRLPATFSASPVAADGKVYLASEDGDVFVVRAGATY
                     ELLATNPMGEPLMATPAISGNMLILRSQNFVYAIGAAGKKG"
     gene            1228..>1991
                     /locus_tag="VGB76_22740"
     CDS             1228..>1991
                     /locus_tag="VGB76_22740"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY0101761.1"
                     /translation="MQPFDFQLRTRLVYGAGALARLGALARELNFRRTLLVADSGLVS
                     SGHVAEASEVLSASNVEVIPFHEFDVNPDTRMCERGRDFAAPLGVDSIIGLGGGSSLD
                     CAKGINFLLTNGGRIQDYQGYGKATRPMLPMIAIPTTAGTGSEAQTYALISDADTHVK
                     MACGDSEAAFRVALLDPALTVSQPAAVTAAAGFDAIAHAVESFVTTRRNAVSDTFARE
                     AWRLLEANYERVLTHPADTEARGAMQLGAHYAGIAI"
BASE COUNT          389 a          693 c          577 g          332 t
ORIGIN      
        1 acaaagtttt cgtcatcacc gccgagagcc gcgatgcgca ggcgcgcttc aacgcgaaag
       61 atcgcggcat cggcctcgca cgcgacgacg cgcgccacgc gtggcgcatc tactcgctcg
      121 accggaacac gggcaagatc aattgggagc gcaccgctta cgaaggcgtg ccgcgcgcca
      181 agcgtcacgt caaagcgacg caggccaact cgacgcccgc gaccgacgga aaaaccatcg
      241 tcgcgctcat gggttccgaa ggtctgtatg cctacgactt cgcgggcaag ctcctctgga
      301 aacaagacct cggcgttttg aatcccggcc tcgcgggcga cccgacttcc gagtggggac
      361 atgcctcttc gcccgtcatc tatcgcggcc tcgtcatcgt ccagtgcgac aagcacagag
      421 actctttcct cgccgcttat catctcaaag acggaaaact cgcgtggcgc gtcgaacgcg
      481 acgaactgcc atcatggagt ccgcccgcga tctacgaagg ccgcacgcgc gccgaactcg
      541 tcacgaacgc gagcaactac atacgcggct acgatccgct cacgggcaaa gaactctggc
      601 gtttcgcaaa cggcgcgacg caggtgcaga tgcccgcgcc cgtcgtagca aacgacatga
      661 tcatcgtgac cggcggttat cccggcaatc gccccgtaca cgctttccgc cccggcgcga
      721 gcggcgacat ctccctgaaa gagggcgagg aatctacgcc cgcgctcgtc tatcgcctgc
      781 cgcgcggctc atcctacacg ggcacgccgc tcgtctatcg tgatctcatg tacatctccg
      841 gcgacaacgg cgtgctgcgc gtctttgaag cgaagaccgg caagcaactg tacgagcaac
      901 gcctgcccgc gaccttcagc gcgtcgcccg tcgcagcgga cggaaaagtt tatctcgcca
      961 gtgaagacgg cgacgtgttc gtcgtgcgcg ccggagcgac ttacgaactg ctcgcgacga
     1021 atccgatggg cgaaccgctg atggcgacgc ccgccatctc cggcaacatg ctcatcctgc
     1081 gctcgcagaa cttcgtctac gccatcggcg cagccgggaa gaaaggatga aggatgaagg
     1141 atgagggatg aatcagagaa ctattcaact ctgttgagtg cttttcttat tcatccctca
     1201 tccctcatcc ctcatccctt aattaagatg cagcctttcg acttccaact tcgcacgcgc
     1261 ctcgtctacg gcgcgggcgc gctcgcgcgg ctcggcgcgc tcgcgcgtga actgaatttc
     1321 cggcgcacgc tcctcgtcgc cgacagcggc ctcgtttcgt ccggccacgt cgcagaggct
     1381 tcggaagtct tgtccgcgtc aaacgtcgaa gtcattccct ttcacgagtt cgacgtgaac
     1441 ccggacacgc gcatgtgcga gcgcgggcgc gacttcgccg cgccgctcgg cgttgactcg
     1501 atcatcggac tcggcggcgg gagttcgctc gactgcgcga agggcatcaa cttcctgtta
     1561 acgaacggcg gcaggattca ggactatcaa ggctacggca aggcgacgcg ccccatgctc
     1621 cccatgatcg ccatcccgac gaccgccgga acgggcagcg aagcgcaaac ttacgcgctc
     1681 atctccgacg cagacacaca cgtcaagatg gcttgcggcg attccgaggc agccttccgc
     1741 gtcgccctgc tcgaccccgc gctcaccgtc tcgcagcccg ccgccgtgac cgccgccgca
     1801 gggttcgacg ccatcgcaca cgccgtcgaa agtttcgtga cgacgcggcg caacgccgtc
     1861 tccgacacgt tcgcgcgcga ggcatggcgg ctgctcgaag cgaattacga gcgcgtgctc
     1921 acgcacccgg cggacacgga agcgcgcggc gcgatgcaac tcggcgcgca ctacgccgga
     1981 atcgccatcg a
//
LOCUS       DASWNS010000657        14125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_51513, whole genome shotgun sequence.
ACCESSION   DASWNS010000657 DASWNS010000000
VERSION     DASWNS010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14125
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51513"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VGB76_22745"
     CDS             complement(<1..793)
                     /locus_tag="VGB76_22745"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0101762.1"
                     /translation="MSVPSLIDQQASRVRPQAARPTAREWARHVGLFLLTATTATIAG
                     TGQALMSANIPENEPNLVAPAHWLGYLWYVPAYYFLSVRELLRQAFAHPELIAQGLTF
                     AFALLAILLAHEAGHYVACRRYKVAATLPFFIPAPPLFLAGTFGAFIKIKSPIPTRRA
                     LFDIGVAGPLAGFVVIIPVAIAALLTATPQPPALFENAAAGTIITFNDPPLLRLFAHA
                     LGVQLNNIAPNPFYFAAWIGLLVTSLNLLPVGQLDGGHAVFAVFGT"
     gene            complement(949..1281)
                     /locus_tag="VGB76_22750"
     CDS             complement(949..1281)
                     /locus_tag="VGB76_22750"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY0101763.1"
                     /translation="MIKLDIVNRVADRTGVPKMKAEQAVDALFHSMKEALARGERIEL
                     RGFGVFVVKPRKRGVGRNPRTGEEVAIPTGKTIRFKPGKELQAHGFDAEDDAGHDTDV
                     PNAATSDE"
     gene            1512..2219
                     /gene="lepB"
                     /locus_tag="VGB76_22755"
     CDS             1512..2219
                     /gene="lepB"
                     /locus_tag="VGB76_22755"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY0101764.1"
                     /translation="MFKRKDGRAPQFGDIGIEDDRDIEVQRLGQESRKHFWSEARLLF
                     RDLVFALLVAALFVVFIVQPVKVEGTSMLPRLHDGERIFVNKLVYYHWPKLDRGDIVV
                     FWFPDDPDKSFIKRVIGLPGEVVEMRGGRIQINGQDLNEPYLDPQLNVGRANLPATLV
                     KPHYYFVMGDNRDNSSDSRLWGQVPEKYIYGKALLRYWPLADATIIRHETDYALTAPS
                     GRPTPYVEPLEDEESEP"
     gene            2270..3568
                     /locus_tag="VGB76_22760"
     CDS             2270..3568
                     /locus_tag="VGB76_22760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101765.1"
                     /translation="MLDLLKPLLLMFYAPARAMGLVRDRAPLGFAAALAIGVKVAYVF
                     YTQWPYLGAQIRLSGLLIGFNILTGTVGSLLLIVVIFVPALIFFANLFERRGSFSLVL
                     QQEFAPAASAVLYAWAAAHLVALPLAALARVSGLEAGWLAQLGQFWGTLGQQQNLPPD
                     AMAQLTDPQQLSLFISSMLVLPFFLLWVVVGVREVFRLSWPRAVAAIVGGGIVMILVS
                     PIMAVLGRFLASPFLLFLLFLLLRGYFGEVMRTQRARASFRQNLEAATLNPADASAHY
                     NLGLIHLQRKELDEAKERFTRAIEIDADEVDAHFQLGRIARMQNDLPGAISRFEQVVV
                     RDESHSQHEIWREIGATYLAAEQYADAYDALERFLARRQSDPEALYLSGRALSGMGRT
                     REAADAMRACIEAVKTAPAYKYRAEKRWLNEAQQFLRTQA"
     gene            3682..4788
                     /gene="carA"
                     /locus_tag="VGB76_22765"
     CDS             3682..4788
                     /gene="carA"
                     /locus_tag="VGB76_22765"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY0101766.1"
                     /translation="MTDVKPALLALEDGRTFRGRSWGAEGEVCGEMVFNTSMSGYQEV
                     LTDPSYAGQIVCMTYPLIGNYGVNALDAESSRPWVEGFVVREASRVASNWRAEETLDA
                     YLKRWRIVAIDHIDTRALVRHIRDRGAMRACLSSVELDAESLVARARSSEAMENRELA
                     SVVTTKETYTVAAEGAERFHVVCYDFGVKLNSLREFARLGCRVTVVPASSPASEVLAL
                     KPDGIFLSNGPGDPASMTKVVEEIARVAGSGVPTFGICFGHQLLGRAFGGSTYKLRFG
                     HRGGNQPVREEATGTIEITSHNHGFAVQADTLPADVEVTHINLNDNCVEGLRHKQLPV
                     FSVQYHPEAAPGPHDAAHHFRRFIELMEQTANKN"
     gene            complement(4920..6182)
                     /locus_tag="VGB76_22770"
     CDS             complement(4920..6182)
                     /locus_tag="VGB76_22770"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0101767.1"
                     /translation="MIERKSTHQQEVTAVAETAAVAAAEAAWPQTRAVLRLILIVLVV
                     AAVIWGLYLLEGVILLLVLSIFFAYLIAPLVEFVHHPVWLRGSERKHFIPRTLAIGVV
                     YLLIFGGLGLAIYLLLPQVGEQITGLGQQAPAYSKSISERAQNLERFYQRIPSPARES
                     VKETVTQTIHTVGEYVQSTAVTTATFALGYAPWLILIPILAFFLLKDADSFRRSALLM
                     LPRGRMRWRGDELFQDVNSTLAAYIRAQLIACLLIGVICTLGFILIGVPYALVLGILA
                     GFLEFIPLAGPLVIFVLAVAVSSFHSPGQALGVFIFLAVLRVVHDYVTYPRIIGQGIH
                     LHPLAVILAILCGAELAGVAGIFLAIPVIAIISVGYRHWMEHKGAEEGIVASLLKPDE
                     AAASPTPQTTTTTPVVAANPERNLPSES"
     gene            complement(6277..6654)
                     /locus_tag="VGB76_22775"
     CDS             complement(6277..6654)
                     /locus_tag="VGB76_22775"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0101768.1"
                     /translation="MSQNFSTEQRTGPRYVISLPVRAEWDDGASGEHVVVEGQTTNVG
                     PMGALVHLEHLPDVGSRVQIAVMREGSPKVSAVAEVLRIERNPAQPLAALQLMNANDE
                     WRGLVWEPSAPHLQHYDYEFEDG"
     gene            complement(6760..8721)
                     /gene="acs"
                     /locus_tag="VGB76_22780"
     CDS             complement(6760..8721)
                     /gene="acs"
                     /locus_tag="VGB76_22780"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY0101769.1"
                     /translation="MSSSTTNIESVLHEERVFPPAQSFSEAAHIKSLADYERLREEAA
                     ASPDAFWARMAEEIHWFKRWETVLRWDAPHAEWFVGGKTNISYNCLDRHLQTWRRNKA
                     AIIWEGEPGEQRTLTYGQLHAEVCRFSNVLKRMNVQPGDRVALYMPLIPELAIAMLAC
                     ARIGATHSIIFGGFSATAIIDRVNDAGCRVVVTADGGWRRGAEVKLKATVDEALTRTP
                     TVEHCIVVRRTGSKVQMQAGRDHWWHELAETVDANCPAVELDSEHPLFILYTSGTTGK
                     PKGILHTTAGYLLWAHLTTKWVFDLKDEDTYWCTADIGWVTGHSYVVYGPLSNGATTL
                     MYEGAPNWPEPDRFWRIVERHRVNIFYTAPTAIRAFIKWGEHWALKHDLSSLRLLGTV
                     GEPINPEAWMWYREIIGGGRCPIVDTWWQTETGGIMISPLPGATPTKPGSATKPLPGI
                     EVDIMSKTGEAVTAAGQGGYLVIKRPWPSMLRTIWNDDERYRAAYWSEIGGVYFAGDG
                     ARRDSDGYYWIMGRVDDVINVSGHRLGTMEIESALVSHEAVAEAAVVARPDELKGSGI
                     VAFVTLEGARQTSDALKEELRRHVAKEIGALARPDEIRFTDQLPKTRSGKIMRRLLRE
                     IAGSGAVSGDTTTLEDFSVLEKLRAQEED"
     gene            8985..10334
                     /locus_tag="VGB76_22785"
     CDS             8985..10334
                     /locus_tag="VGB76_22785"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY0101770.1"
                     /translation="MNQTKSFLRLASILLLLAAFVSPALPQSKETTKAAPVTDTLRPS
                     TATSNAPRDERLWQRALKLQRSAIVVDSHNDILSFMFDENYDIGQSSVGKYHTDIARM
                     KQGGLSAEFFSVYVDRTYAAKGGAARRALDLIDMVYRAAEKYPNDLVMSYSVADIRRA
                     KKGKKIAALMGIEGGHAIENSLMALRDFYRLGIRYMTLTHNNTNDWADACCDAARHNG
                     LSDFGKEVVREMNRIGMLVDISHVSDKTMSDVLDISTAPVIASHSSARALAARPRNIP
                     DDLLRRIAKNGGVVMINFYPIFLDQKAIDASAARDARLKPERERINETFKNDPKRREE
                     ELDRLNAANPLPPTPLSVLVDHFDHVAKVAGIDHVGIGSDFDGVSYLPEGMKDIAQLP
                     NLTYELLRRGYSEGDVRKVLGENFLRAFATAERAARTSSGGQVSRDGSVRRIDEGKR"
     gene            10665..11840
                     /gene="hemW"
                     /locus_tag="VGB76_22790"
     CDS             10665..11840
                     /gene="hemW"
                     /locus_tag="VGB76_22790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY0101771.1"
                     /translation="MKRAGIYIHIPFCRSRCSYCDFATGAYEGQLAERYVCALTKEIE
                     EFEHAPLPETPLSNDLRRPPSLDVDTIYFGGGTPSLLTPAQVERILRAVQERFRVAPG
                     SEVTLELNPGTVTRESVEQFRAAGINRASFGLQTFDDEQLRRLGRTHTAADARRTLST
                     LREAGFDNVSFDLIAGLPGQTLPEWAENMEAALALRPAHLSLYLLEVHSGTPLAAQLR
                     EGRWPQPDPDVAAEMYQLLIERTRAAGYEHYEISNFCLPGHEARHNLKYWTGAPYYGF
                     GSSAHSYDGERLRWSNERDAARYCELLETRGSAVVETNELSARDASAEALFLGLRLMR
                     GVDLEAHQTQFGTDVRHEYAADLSRFREAELIEIDGGLLRLTPAGVLLSNEVFAAFI"
     gene            11962..13008
                     /locus_tag="VGB76_22795"
     CDS             11962..13008
                     /locus_tag="VGB76_22795"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEY0101772.1"
                     /translation="MKLIDLRSDTVTKPTPAMRRAMAEAEVGDDVYGEDPTVNRLQER
                     AAEIFRKEAALFVPTGSMGNQIAVKLHTRPGTEVVIEERGHIFNYEMAAMSAIAGTIA
                     RPVKSTDESGILRWDDIKAAIHANDAPYYVAPTGLVALENSHNLAGGTLLSRARTEEI
                     CDSAHALRIPVHLDGARIFNAAAALDETVAALARPCDSVMFCLSKGLGAPVGSVLLGG
                     LEFIEEARRWRKLLGGGMRQAGVIAAAGLVALEESPARLPADHANARRLAEALAELPG
                     VKIDPARVVTNIVIFDIEGTRRAANDICARLQTQGVLASGFGSSIRMVTHYDVSTADI
                     DAAINALREVLREG"
     gene            13149..13832
                     /gene="cmk"
                     /locus_tag="VGB76_22800"
     CDS             13149..13832
                     /gene="cmk"
                     /locus_tag="VGB76_22800"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY0101773.1"
                     /translation="MKPIIIAIDGPSGAGKSTLGRRLARELNLLYVDTGAMYRAAALA
                     VVAANVNATDADEVARIAAAARIELAGDPDSLRVLLDGRDVSAEIRSEQITRLSSVIS
                     AIPAVRRDLVRRQREMGAQGGGVVLDGRDIGTVVFPQADVKFFLTAVPEERAKRRFDE
                     DHRTEPDLTFQETLDDINTRDERDSTRADSPLRIAEDAVVIDTTDLSIEQVFARMLEV
                     VREREMGSV"
     gene            13837..>14125
                     /locus_tag="VGB76_22805"
     CDS             13837..>14125
                     /locus_tag="VGB76_22805"
                     /inference="COORDINATES: protein motif:HMM:NF012881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY0101774.1"
                     /translation="MFTGIIEELGRVRGLERRGEGVRLTVEARTVTEGTREGDSISVN
                     GVCLTALEVSRESFAADGSRETLQRSTLGSLRAGSFVNLERAVTPETRLGGL"
BASE COUNT         2745 a         4449 c         4358 g         2573 t
ORIGIN      
        1 gcgtgccgaa gacggcgaag accgcgtgcc cgccgtcgag ttgtcccacg ggcagcaggt
       61 tcaaactcgt cacgagcagt ccgatccacg cggcgaagta gaaggggttg ggcgcgatgt
      121 tgttgagttg gacgccgagc gcgtgcgcga agaggcgcag gagcggcggg tcgttgaaag
      181 tgatgatggt gcccgccgcc gcgttctcga agagcgcggg cggctgcggg gtggcggtca
      241 acagcgcggc gatggcgacg gggatgatga cgacgaaacc cgcgagtggt ccggcgacgc
      301 cgatgtcgaa gagggcgcgt cgcgtcggga tgggagattt gatcttgatg aacgcgccga
      361 acgtgcccgc gagaaagagc ggcggcgcgg ggatgaagaa ggggagcgtg gccgccactt
      421 tgtaacggcg gcaggccacg taatgcccgg cctcgtgcgc gagcaggatg gcgaggagcg
      481 cgaaggcaaa cgtcaagccc tgcgcgatca actccggatg cgcgaacgcc tgccggagaa
      541 gttcgcggac ggagagaaag tagtaggccg gaacatacca gaggtagccg agccaatgtg
      601 cgggcgcgac gaggttcggc tcgttctcag gaatattcgc ggacatgaga gcctgtccgg
      661 tgcccgcgat ggtggccgtc gtggcggtca gcaaaaagag gccgacgtgg cgcgcccatt
      721 cgcgcgcagt cggtcgcgct gcttgcggtc gaacccttga tgcctgttga tcgatgaggg
      781 acgggacgga catgataaag cagtgacaag tgacgggtga caagtggaaa ggccgaaaaa
      841 agtgaatcgt gaatcgtcga gcgtgaatag ttaaaaccgt cttcgtctat tcacaattga
      901 caattcacga ttcgcgatca cgtgtgacga ttgactcgtc ttatctcgtc actcgtcact
      961 cgttgctgca ttcgggacat cggtgtcgtg tccggcgtcg tcttccgcgt cgaagccgtg
     1021 ggcttggagt tccttgccgg gcttgaagcg gatggtcttg ccggtcggga tggcgacctc
     1081 ttcgccggtg cgcgggttgc gaccgacgcc gcgcttgcgt ggtttgacga cgaagacgcc
     1141 gaagccgcgc agttcaatgc gttcgccgcg tgcgagcgct tctttcatcg aatgaaagag
     1201 ggcgtcaacg gcctgttcgg ccttcatctt cgggacgccc gtgcgatctg ccacgcggtt
     1261 gacgatgtcg agtttaatca cgcttgaatt tagcctccgt tcgggtctgc acggctcggg
     1321 cgttggtctg taaagtatag acgccgcttg ataagcgata atagagacgc gccgtaaagg
     1381 ctgtcaagca aacacccgga gcgcgcctgc gccccctcgc gttgacaaag cgcgcggcgc
     1441 gcggctaacc tcgaaacaag ggattttagg agcgacgaga gacgacggaa ggcgacaaac
     1501 ttaagaagcg tatgttcaag cgtaaggacg ggcgtgcgcc ccagttcggc gacatcggga
     1561 ttgaggacga ccgcgacatc gaagtgcagc gactcggaca ggaatcgcgc aagcacttct
     1621 ggtcggaagc gcgcctgttg tttcgcgatc tcgtcttcgc cctcctcgtc gccgcgctct
     1681 tcgtcgtctt catcgtgcag cccgtcaaag tcgaaggcac ttcgatgctg ccgcgcctcc
     1741 acgacggcga gcgcatcttc gtcaacaaac tcgtctatta ccactggccg aaactcgacc
     1801 ggggcgacat cgtcgtcttc tggtttccag acgacccgga taagagtttc atcaagcgcg
     1861 tcataggctt gccgggcgag gtggtggaga tgcgcggcgg tcgcatccag atcaacgggc
     1921 aggatttaaa cgagccgtat ctcgacccgc aactcaacgt cgggcgcgcg aatctgcccg
     1981 ccacgctcgt caagccccat tactatttcg tgatgggcga caaccgcgac aattcgagcg
     2041 actcgcgcct ctgggggcaa gtgcccgaaa agtatatcta cggcaaagcc ctcctgcgct
     2101 actggccgct cgccgacgcc accatcatcc gccacgaaac cgactacgcg ctcaccgcgc
     2161 cgagcggcag gccgacgccc tacgtcgagc cgctggagga tgaagaaagt gaaccttaag
     2221 agccgtcggt ttttgtccga ctgtctggct gacggctgac agctatttta tgctcgacct
     2281 cttgaagccg ctgctgttga tgttctacgc gcccgcgcgc gcgatgggac tcgtgcgcga
     2341 ccgcgcgccg ctcggcttcg ccgccgcgct cgccatcggg gtgaaagtgg cttacgtgtt
     2401 ctacacgcaa tggccttatc tcggcgcgca aattcgtctg agcggcctgc tcatcgggtt
     2461 caacatcctg accggaacgg tcggcagcct gctcttgatc gtcgtcatct tcgtcccggc
     2521 attgatcttc ttcgccaacc tgttcgagcg caggggcagt ttcagcctcg tgctgcaaca
     2581 ggagttcgcg cctgccgcct ccgccgtcct ctacgcatgg gcggcggcgc acctcgtcgc
     2641 tttgccgctg gcagccctcg cacgcgtgag cggcctcgaa gctggctggc tcgcgcagct
     2701 ggggcagttc tgggggacgc tcggccagca acagaacctg ccgcccgatg cgatggctca
     2761 actcacagac ccgcaacaac tctccctctt catctcgtcc atgctcgtgc tgcccttctt
     2821 cctgctgtgg gtcgtcgtcg gcgtgcgcga agtgtttcgt ctctcgtggc cgcgcgccgt
     2881 agcggcgatt gtggggggcg ggatcgtcat gatcctcgtc tctcccatca tggcggttct
     2941 gggtcgtttt ctcgcctcgc cgttcctgct gttcctgctg ttcctgctgc tgcgcggcta
     3001 cttcggcgaa gtgatgcgga cgcaacgcgc gcgcgcctcg tttcgccaga acctcgaagc
     3061 cgcgacgctc aacccggctg acgcttccgc acactacaac ctcggcctca tccatctcca
     3121 acgcaaagaa ctcgacgagg ccaaagagcg tttcacgcgc gccatcgaga tcgacgcaga
     3181 cgaagtggac gctcattttc aactcggtcg catcgcccgg atgcagaacg atctccccgg
     3241 cgcgatcagt cgtttcgaac aggtcgtcgt gcgcgatgaa tcacactcgc agcacgaaat
     3301 ctggcgcgag atcggcgcga cttatctggc cgcagagcag tacgcggacg cttacgacgc
     3361 gctcgaacgc tttctcgcgc gccgtcaatc agaccccgaa gcgttgtacc tgtccggacg
     3421 cgcgctctcc ggcatgggac gcacgcgcga agccgcagac gcgatgcgcg cctgcatcga
     3481 agccgtcaaa actgcgcccg cctacaaata tcgcgccgaa aaacgctggc tcaatgaagc
     3541 ccaacagttc ttgcggacgc aggcttaaaa gccgccagtt gccctctgcc tgcgccctct
     3601 gcctctgccc ggcgcgtgag tggtcggcga ttagagcgaa cgactggcaa cgaacgacgg
     3661 acgaccggca gagatcaaat tatgactgac gttaaacccg cgctccttgc gctcgaagat
     3721 ggacgaacgt ttcgcggtcg ctcgtggggc gcggagggcg aagtgtgcgg cgagatggtc
     3781 ttcaacactt cgatgtccgg ttatcaggaa gtcctgaccg acccttccta tgcggggcag
     3841 atcgtgtgca tgacttatcc gctcatcggc aactacggcg tgaacgcctt ggacgccgaa
     3901 tcgagccgcc cgtgggtgga gggtttcgtc gtgcgcgagg cgagccgcgt ggcttccaac
     3961 tggcgcgccg aagagacgct cgacgcctac ctcaagcgtt ggcggatcgt cgccatcgac
     4021 cacatcgaca cgcgcgctct ggtccggcac atacgcgacc gtggcgcgat gcgcgcgtgc
     4081 ctgtcgagcg tcgaactcga cgcggagagt ctggtcgcgc gtgcgcgctc gtccgaggcg
     4141 atggagaaca gggaactggc gagcgtcgtg acgacgaagg agacttacac ggttgcggcg
     4201 gaaggcgcgg agcgtttcca cgtcgtctgc tacgacttcg gcgtgaagct gaattctctg
     4261 cgtgagtttg cccgcctcgg ttgtcgcgtg acggttgtgc ccgcttccag tcccgcgtcg
     4321 gaagtgctgg ccttgaagcc ggacgggatc tttctctcga acgggccggg cgatccggct
     4381 tcgatgacga aggtggtcga agagatcgca cgcgtcgccg gatcgggcgt gcccaccttc
     4441 ggcatctgct tcggccatca actgctgggg cgagccttcg gcggttcgac ttacaaacta
     4501 cgcttcggtc atcgcggcgg caaccaaccc gtgcgcgaag aggcgaccgg caccatcgag
     4561 atcacttcgc acaatcacgg cttcgccgtg caggccgaca cgctcccggc ggacgtggaa
     4621 gtcacgcaca tcaatctcaa cgacaactgc gtggaaggct tgcgccacaa gcagttgccc
     4681 gtcttctccg tgcagtatca ccccgaagcc gcccccggcc cccacgacgc cgcccaccac
     4741 ttccgccgct tcatcgaact catggagcag acggcgaata aaaactgaac gcgccgggaa
     4801 aagaaaagca ttcaccccgg agacacagag ggcacagaga gtcgcaatct tctctgtgcc
     4861 cccttcgtgt ccttaccgtg tctctgtggt gagtcttaag ttctgacctg tcgcccgctt
     4921 taactttcgc tcggtagatt ccgttccggg tttgccgcaa cgacgggagt tgtcgttgtc
     4981 gtctgcggcg ttggcgacgc ggcagcttcg tcgggcttga ggagggaggc gacgatgcct
     5041 tcttccgcgc ccttgtgttc catccagtga cggtagccga cggagatgat ggcgatgacg
     5101 gggatggcga ggaagattcc ggcgacgccc gccagttccg ctccgcagag aatggcgagg
     5161 ataaccgcca gcgggtggag gtgtatgccc tgaccgatga tgcgcgggta ggtcacgtag
     5221 tcgtggacga cgcgcaggac ggcgagaaaa atgaagacgc cgagagcctg tcccggcgag
     5281 tggaagctcg acaccgcgac ggctagaacg aagatcacga gtggtccggc gagcgggatg
     5341 aattccagaa aacctgcgag gatgccgagc acgagtgcgt aaggcacgcc gatcaggata
     5401 aagccgagcg tgcagatcac gccgattaaa aggcacgcga tgagttgcgc gcggatgtag
     5461 gcggcgagcg tgctgttgac atcctgaaac agttcgtcgc cgcgccagcg catacgcccg
     5521 cgcgggagca tcaacagggc ggaacgacga aagctgtccg cgtctttcag cagaaagaag
     5581 gcgaggatgg gaatcaggat cagccacggc gcgtagccga gggcgaaagt cgccgtcgtg
     5641 accgccgtgc tttgcacgta ctcgccgacc gtgtgaatgg tctgtgtgac agtctctttg
     5701 acggactcgc gtgcgggcga agggatgcgt tgatagaacc gctccaaatt ttgcgcgcgt
     5761 tccgagatgc ttttcgaata ggctggagcc tgctgtccga gtcctgtgat ctgttcgccg
     5821 acctgcggca gcaggaggta gatagccagg ccgaggccgc cgaagatgag tagatagacg
     5881 acgccgatag cgagcgtgcg cgggatgaaa tgcttgcgct cgctgccgcg cagccagacc
     5941 gggtggtgga cgaattccac gagcggcgcg atcaggtaag cgaagaagat cgagaggacg
     6001 agcaggagga tcacgccttc gagcaggtag agtccccaga tgaccgccgc cacgacgagc
     6061 acgatcagga tcaggcgcag caccgcgcgc gtctgcggcc aagcggcttc cgccgccgcg
     6121 accgccgccg tctccgcaac tgccgtcacc tcttgctggt gtgtgctctt ccgctcaatc
     6181 ataaatgtaa ggatgaagga tgaaggatga acgacgaatg aaagcactca agcgagttaa
     6241 tgctttcatg catcgttcat cgttcaacct ttcatctcag ccgtcttcaa actcgtagtc
     6301 gtagtgttgg aggtgcgggg cggaaggttc ccagacgagg ccgcgccatt cgtcgttggc
     6361 gttcatgagt tggagtgcgg cgagcggttg cgccgggttg cgctcgatgc gcaagacctc
     6421 cgccaccgcc gacaccttcg gcgatccctc gcgcatcacc gcgatctgca cgcggctgcc
     6481 cacatccggc aaatgctcca gatgcaccag cgcgcccatc ggcccgacgt tcgtggtctg
     6541 tccttcgacg acgacgtgct cgcccgatgc gccgtcatcc cattcggcgc gcaccggcaa
     6601 actgatcaca taacgcggtc ccgtccgctg ctcggtcgag aaattttgag acataaagta
     6661 accggtgatg agtgatgagt gatgagtgat gagtaaaaac aaaatacctt tgtctttcac
     6721 tcatcactca tcactcatca ctcatcactt cctttctgtt tagtcttctt cctgcgcacg
     6781 cagtttttcg agtacggaga aatcttcgag ggtcgtcgtg tcgccggaaa ccgcgccgct
     6841 gcctgcgatt tcgcgcagca ggcggcgcat gatcttgccc gaacgagtct tcggcagttg
     6901 atccgtgaac ctgatctcgt cgggacgcgc cagcgcgccg atctcctttg cgacgtggcg
     6961 gcgcaactct tctttcaacg catccgaagt ttgccgcgcg ccttcgagcg tgacgaaggc
     7021 gacgatgccc gaacctttca gttcgtccgg acgcgccacg accgccgctt ccgcgaccgc
     7081 ctcatgtgag acgagcgcgc tctcgatctc catcgtaccc aaacgatgcc cgcttacatt
     7141 aatgacatca tcaacgcgcc ccattatcca gtaataaccg tcggaatctc gccgcgcgcc
     7201 gtcgcccgcg aaatagacgc ccccaatctc cgaccagtag gccgcgcggt agcgttcgtc
     7261 gtcgttccag atcgtgcgca gcatcgaagg ccacggtcgt ttgatgacga gataaccgcc
     7321 ctgtcccgcc gccgtcacgg cttcgcccgt cttcgacatg atgtcaactt cgatgccggg
     7381 cagcggcttc gtggccgaac cgggtttggt gggcgtcgca ccgggcagcg gcgagatcat
     7441 gatgccgccc gtctccgtct gccaccacgt atcgacaatc gggcaacgcc cgccgccgat
     7501 aatctccctg taccacatcc aagcctccgg gttgatcggt tcgccgaccg tgccgagcag
     7561 gcgcaaggat gagaggtcgt gtttcaaagc ccagtgctcg ccccatttga tgaaggcgcg
     7621 gatggcggtc ggcgcggtgt agaagatgtt gacgcgatgg cgttcgacga ttcgccagaa
     7681 tctgtccggc tcaggccagt tcggcgcgcc ttcatacatc aaagtcgtcg cgccgttcga
     7741 gagcggtcca tagacgacgt aggaatgacc cgtcacccag ccgatgtcag ccgtgcacca
     7801 gtaggtgtct tcgtccttga gatcaaagac ccacttcgtc gtgaggtgcg cccagagcag
     7861 gtagcctgcg gtcgtgtgca ggatgccttt cggtttgccg gtcgtgccgg aggtgtagag
     7921 gataaagagc gggtgctcgc tatcgagttc gacggcggga cagttggcgt caaccgtctc
     7981 cgcgagttcg tgccaccagt gatcgcgccc cgcctgcatc tgaactttcg accccgtgcg
     8041 gcgcacgacg atgcagtgct cgacggtcgg cgtgcgggtc agagcctcgt ccaccgtcgc
     8101 cttcaacttc acttccgcgc cgcgccgcca accgccgtca gccgtgacga cgacgcgaca
     8161 gcccgcatcg ttgacgcgat caatgatcgc cgtcgcggag aagccgccga agatgatcga
     8221 atgggttgcg ccgatgcgcg cgcaggcgag catggcgatg gcgagttcgg gaatgagcgg
     8281 catgtagagc gcgacgcggt cgcccggttg cacgttcatg cgcttgagga cgttcgagaa
     8341 gcggcagacc tccgcgtgga gttgcccgta ggtgagcgtg cgctgttcgc cgggttcgcc
     8401 ctcccagatg atcgccgcct tgttgcgtcg ccacgtctgt agatgacggt cgagacagtt
     8461 gtaggagatg ttcgtcttgc cgccgacgaa ccactcggcg tgcggcgcgt cccagcgcaa
     8521 aaccgtctcc cagcgtttga accagtgaat ctcttcggcc atgcgcgccc agaaggcgtc
     8581 gggcgaggcg gcggcctctt cgcgcagacg ttcgtagtcc gccaaagatt tgatgtgcgc
     8641 cgcctcggag aaactctgcg cgggcgggaa gacgcgctcc tcgtgcagca ccgattcgat
     8701 gttcgtcgtc gaggaagaca taaactctgg ctcgaatctc ctccctgaaa agttggcggc
     8761 gcgcgctctg gctcacgccc tgtaattaaa attgcgtttc tggtgagtgt cctgtgtatc
     8821 acagaaccgc tcgacgcgac aatgacttct gaacagcacg cgccgtgcca cgaggccgac
     8881 gcgccgcacg cgctttctca gccgtcgctt gtctggcgca agcctctccg ttaaagtacg
     8941 cccgatcccg tacaccccga aagcgaaccg gaagtcacca caccatgaat cagacgaaat
     9001 cttttctccg cctcgcctca atcttgctgt tgctcgccgc cttcgtctca cccgccctgc
     9061 cgcaaagcaa agagacgacg aaggccgcgc ccgtgacaga cacgctgcgc ccctcgaccg
     9121 ccacatcgaa tgccccgcgc gacgaacgtt tgtggcagcg cgcgctcaaa ctgcaacgct
     9181 cggctatcgt cgttgactcg cacaacgaca tcctctcctt catgttcgat gagaactacg
     9241 acatcggcca gtcgtccgtc ggcaagtatc acacggacat cgcgcgcatg aagcagggcg
     9301 gactctcggc ggagttcttc tccgtctacg tggatcgtac ctacgccgcg aaaggcggcg
     9361 cggcgcggcg cgcgctggat ctgatcgata tggtttatcg cgcggcggaa aagtatccga
     9421 acgatctcgt gatgtcttac tcggtggcag acattcgccg cgcgaagaag gggaagaaga
     9481 tcgccgcgct gatgggcatc gaaggcggcc acgcgattga aaattcgctg atggccttgc
     9541 gcgatttcta ccgcctcggc atacgctaca tgacgctcac gcacaacaac acgaacgatt
     9601 gggcggacgc ctgctgcgac gccgcgagac acaacggcct ctcggatttc ggcaaggagg
     9661 tcgtgcgcga gatgaaccgc atcggcatgc tcgtggacat ctcgcacgtc tccgacaaga
     9721 ccatgtccga cgtgctcgac atctcgaccg cgcccgtcat cgcttcgcac tcctcggcgc
     9781 gcgccctggc tgcccgcccg cgcaacatcc ccgacgactt gctccgccgc atcgcgaaga
     9841 acggcggcgt cgtgatgatc aacttctatc ccatcttcct cgaccaaaaa gcgattgacg
     9901 cgagcgcggc gcgcgatgcg cggctcaagc ccgagcgcga acggatcaac gagacgttca
     9961 aaaacgatcc gaagcgacgc gaagaggaac tggacaggtt gaacgcggcg aacccgctgc
    10021 cgccgacgcc gctctccgtc ctcgtggatc atttcgatca tgtcgccaaa gtcgcaggca
    10081 tcgatcacgt cggcatcggt tccgacttcg acggcgtctc ctatcttccc gaaggcatga
    10141 aagacatcgc gcaactcccc aatcttacct acgaactcct gcgtcgcggt tacagcgaag
    10201 gcgacgtgcg caaagtgctc ggcgaaaatt tcctgcgcgc cttcgccacc gccgaacgcg
    10261 ccgcacgcac ctcgtccggc ggtcaggtca gccgcgacgg cagcgtccgg cgaatagatg
    10321 aagggaagag gtaaaggcaa acgatgaagg agaagacaga acacagaata caggagtcag
    10381 aagcaagaga cgcgcacctt ttatttttta tcaatcggcg tgccgtgtga ggcgcgaaaa
    10441 accgctccgc gatttatccg aaattacgcg gggaggcgac ggaaaacatg tccaaacttc
    10501 gcccgcctgc acgcatcgct cacacaaaag tcgtacaccc gattttaaaa ataatacacg
    10561 cggcgcattc aataagttgg tcaagtatgt cgtgatcgca atcgcacgta aaagcggctg
    10621 cgagcttaac tcgacgcgtg cgcgtgctat aatttttctc tccaatgaag cgcgccggaa
    10681 tctacataca tatcccgttc tgtcgttcgc gctgctccta ttgcgacttc gccacagggg
    10741 catacgaagg gcaactcgcc gaacgctacg tctgcgcgct gacgaaagag atcgaagagt
    10801 tcgagcacgc gccgctgccg gagacgcctc tctcgaacga cctgcgccgc ccgccctctc
    10861 tcgacgttga cacgatctat ttcggcggcg gcacgccgag cttgctcacg cccgcgcagg
    10921 tcgagcgcat tctgcgagcg gtgcaggagc gtttccgcgt cgcgcccggt tcggaagtga
    10981 cgctggagtt gaaccccggc acggtgacgc gcgagagcgt cgagcagttt cgcgccgcag
    11041 gcatcaaccg cgcgagcttc ggcctgcaaa ctttcgacga cgagcaactg cgacgcctcg
    11101 ggcgcacgca caccgccgca gacgcgcgcc gcactctctc caccctgcgc gaagccggat
    11161 tcgataacgt cagtttcgat ctcatcgcgg ggctgcccgg ccagacgttg ccagagtggg
    11221 ctgagaacat ggaggccgcg ctcgcgcttc gccccgcgca tctctcgctc tatctgctcg
    11281 aagtgcacag cggcacgccg ctcgccgcgc aactccgcga aggccgctgg ccgcaacccg
    11341 acccggacgt ggccgccgaa atgtatcaac tgctcatcga gcgcacgcgc gccgccggtt
    11401 acgagcacta cgagatctcc aacttctgcc tgcccggaca cgaagcgcgc cacaacttga
    11461 aatactggac gggcgcgcct tactacggct tcggttcgtc ggcgcattcc tacgacggcg
    11521 aacgtctgcg ctggtcgaac gaacgcgatg cggcgcgcta ctgcgaactg ttggagacgc
    11581 gcggcagcgc agtggtcgaa acaaacgaat tgagcgcgcg cgacgcgagc gcggaagctc
    11641 tcttcctcgg cctccggctg atgcgcggcg tcgatctcga agcgcaccag actcaattcg
    11701 gaaccgacgt gcgacacgaa tacgccgcag acctctcacg cttccgcgaa gccgagttga
    11761 tcgaaataga cggcggcctt ctacgcctca cgcccgcagg cgtgctgctc tcgaatgaag
    11821 tcttcgccgc attcatctaa gatggataaa tatgaaacag gcgaccgggt atggacgccg
    11881 gagcagtcgt ggactaactc aacgaacgat tgggcgtgag tttgaaatca aattaacagg
    11941 caggacgagc aggcggcttt tatgaagttg atcgatctac gcagcgacac tgtgacgaaa
    12001 ccgacgcccg cgatgcggcg cgcgatggcc gaggccgaag tgggcgacga cgtgtacggg
    12061 gaagacccga cggtcaaccg cttgcaggag cgcgcggcgg agatttttcg caaagaggcc
    12121 gcgctctttg tgccgacggg ttcgatgggc aaccagatcg ccgtcaagtt gcacacgcga
    12181 ccgggcacgg aggtcgtcat cgaagaacgc ggccacatct tcaactacga gatggccgcg
    12241 atgtccgcca tcgcaggcac catcgcgcgc cccgtcaaaa gcacggacga gagcggcatc
    12301 ctccgttggg acgacatcaa ggcggccatt cacgccaacg acgcgcccta ctacgtcgcg
    12361 ccgaccggac tcgtcgcgct cgaaaattcg cacaacctcg cgggcggaac gctgctgtcg
    12421 cgcgcacgca ccgaagagat ttgcgactcc gcgcacgcgc tgcgtattcc cgtccacctc
    12481 gacggcgcgc gcatcttcaa cgccgccgcc gcgctcgacg agacggtcgc cgcgctcgcg
    12541 cgtccctgtg attcggtgat gttctgtctc tcgaaaggat tgggcgcgcc cgtcggctcg
    12601 gtgctcctcg gagggcttga gtttattgaa gaggcgcgtc gctggcgcaa actgctcggc
    12661 ggcgggatgc gacaggcggg cgtcatcgcc gccgccggac tcgtcgcgct cgaagaatcg
    12721 cccgcgcgcc tgcccgcaga ccacgcaaac gcgcgtcgcc tcgccgaagc gttggccgaa
    12781 ctgccgggcg tgaagattga ccccgcacgc gtcgtcacca acattgtcat cttcgacata
    12841 gagggcacgc ggcgcgctgc gaacgacatc tgcgcgcgcc tccaaacaca gggcgtcctc
    12901 gcctccggct tcggctcgtc aatccgcatg gtcacgcact acgacgtttc gacggcagac
    12961 atcgacgccg ccatcaacgc cctccgcgaa gtgctgcgcg aaggatagaa cgcgcgaagg
    13021 atagaaagcc cggcggtcgt ccgtcggcgg ttgtcagtta ttagttactg acagttacgc
    13081 cgcttgagat tgatttagga ttaataacag gtcatagcta atagccggta gccgctaaca
    13141 gcctctttat gaaaccgatc atcatcgcca ttgacgggcc ttcgggcgcg ggcaagtcaa
    13201 cgctggggcg caggctggcg cgcgaactga atctgctcta cgtggatacg ggcgcgatgt
    13261 atcgcgccgc cgcgctcgcc gtcgtcgccg caaacgtgaa cgcgacggac gcagacgaag
    13321 tggcgcgcat cgccgccgcc gcgcgcatcg aactcgcggg cgacccggac agcctgcgcg
    13381 tcctgctcga cgggcgcgac gtgtccgcag agattcgcag cgagcagatc acgcggctct
    13441 cttccgtcat ctccgcgatt ccggcggtgc gccgcgatct cgtccgccgc cagcgcgaga
    13501 tgggcgcgca agggggcggc gtcgtcctcg acgggcgcga catcggcacg gtcgtcttcc
    13561 cgcaggccga cgtgaaattc tttctcaccg ccgtccccga agaacgcgcc aagcgacgct
    13621 tcgacgaaga ccatcgcacc gaacccgacc tcacttttca ggaaaccctc gacgacatca
    13681 acacgcgcga cgaacgcgac tccacacgcg ccgactcgcc cctgcgcatc gccgaagatg
    13741 ccgtcgtcat agacaccacc gatctctcca tcgaacaggt cttcgcgcgc atgctcgaag
    13801 tcgtccggga acgcgagatg ggcagcgtat aaaaatatgt tcacaggcat catcgaagag
    13861 ttggggcgcg tgcgcggttt ggagcggcgc ggcgagggcg tgcggctgac ggtcgaggcg
    13921 cggacggtga ccgaagggac gcgcgagggc gattccatct ccgtcaacgg cgtgtgtctg
    13981 accgcgctcg aagtgagccg tgaaagtttc gcggcggacg gctcgcggga gactttgcag
    14041 cgttcgacgc tggggagtct gcgcgccgga tcgttcgtca atctcgaacg cgccgtaacc
    14101 cctgagacga gactcggcgg cctca
//
LOCUS       DASWNS010000658         5780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_466083, whole genome shotgun sequence.
ACCESSION   DASWNS010000658 DASWNS010000000
VERSION     DASWNS010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466083"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VGB76_22810"
     CDS             <1..648
                     /locus_tag="VGB76_22810"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0101775.1"
                     /translation="LVLGQTSLDLFIHDGYFVLAHFHLVMGVAAIFGMFAGVYFWFPK
                     MFGRMMSERLGQLHFWPTFIGVYGIFVPMHIMGIVGMPRRYSSFQEYDFLRPLDPLVL
                     LVTIAAIVTAVAQFIFLFNFIYSIFKGERASGNPWEATTLEWVTPTTPPPHDNFAGHI
                     PTVYRGAYEFAVPGAPEDYVLQTTPDAEDENITPAEQTLAQGTQGSSGNGHNGHH"
     gene            626..1378
                     /locus_tag="VGB76_22815"
     CDS             626..1378
                     /locus_tag="VGB76_22815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101776.1"
                     /translation="MVTTVTTKNSGSVSTTTKPLNVGGTGRRPGGNGSRGGPQPPNGN
                     DGERYGRAPRDATPQQYRIGMWLALSAILMMFAALTSAYVFRSTRTQQEWQVFAVPQM
                     LWVSTLMILASSATFEAARRAEQRGRHGLYRNWLLVSLLLGFGFLASQLLAWRELVGQ
                     GIYLASNPHSSFFYLLTGLHALHLLGGILGLSYLAISARRKTAPPGDTLDVPDAGNRD
                     RKRRASVDAIGLYWHFMDGLWVYLFALLFLWR"
     gene            1382..2047
                     /locus_tag="VGB76_22820"
     CDS             1382..2047
                     /locus_tag="VGB76_22820"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY0101777.1"
                     /translation="MSTHAQTEHLIADDWSGGTFPYHVGHKKLGMWLFIVSDALTFSA
                     LLIGYSYMRVSSDQWPTPFAFYPSIIFSTVMTLCLLASSFTMVMAVAASARRDRRKAR
                     RWIGATILGGLTFLVLHIYEWLHLINDYGLKPFTLPAAWIERWPQASPLFGATFFAIT
                     GLHMFHVLTGVIYLGVIALRIRRKTHEDVEISGLYWHFVDLVWMFVFPLIYLLSVDFH
                     PVH"
     gene            2096..2680
                     /locus_tag="VGB76_22825"
     CDS             2096..2680
                     /locus_tag="VGB76_22825"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEY0101778.1"
                     /translation="MSTRFGALGNLMKKQDLILNGVCLLFATGFLAMALSNVRWAGNF
                     FSIDSLFFTLVSLTLAGIFLAIPLYWAYETGMLRDFFAAEDETTLAAVDAAPVHFEGT
                     TKLFLSILVYLLVLTAIEVALAYVQVPLALMLTLLVGLSLVKAALILAYFMHLRYERL
                     SLVYTLIPMLVICICLLFIFFPDSIRLLNLRIYK"
     gene            2693..2944
                     /locus_tag="VGB76_22830"
     CDS             2693..2944
                     /locus_tag="VGB76_22830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101779.1"
                     /translation="MKIEPAARTLLLLALVYAAWAGFFATDALAQCAMCKASAANLDS
                     ISARQLNISTLILLCPPVAIFCAIFVVAYKHRNPPDDES"
     gene            2963..3208
                     /locus_tag="VGB76_22835"
     CDS             2963..3208
                     /locus_tag="VGB76_22835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101780.1"
                     /translation="MANDNEALALNNDHQSIRGRACPQCGGKIIPSDRAVYESKNDPA
                     SVFPLWQCERCGYEQMSERQSAPKKAAPHAAAKKRAE"
     gene            3348..3773
                     /locus_tag="VGB76_22840"
     CDS             3348..3773
                     /locus_tag="VGB76_22840"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEY0101781.1"
                     /translation="MDYSKLPHINALLNATSGLLLLAGFYFISRRRITAHLRCMTAAL
                     AVSCIFLASYLVYHYNHGSTRFTGQGYVRPVYFFILLTHTILAAIIVPLIAVTLRRAW
                     RGDFGRHRKIARWTFPLWLYVSVTGVIVYLMLYQLYPSR"
     gene            3815..4714
                     /locus_tag="VGB76_22845"
     CDS             3815..4714
                     /locus_tag="VGB76_22845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY0101782.1"
                     /translation="MDGDNSNNHHTRKHPAPDTADAPAATTARRRSPWPLAVVAALFI
                     ILPFLSWYGTTFWRPLNDEEIEKYLADTEKPRHVQHALEQIDKRIIAGDGEARKWYPR
                     VVGVAGNPLPDLRMTAAWVMGDDNKSEEFHVALKSLLADSEPAVRRMAALSLSRFNDG
                     SGRAELISMLRPFSVKLPQAGTLLTVLPVNSPVTREAMLARVRDEKGAVQEVRAPVAG
                     KIERVSVTDGTSVAAGTELLVLAPDGKNVWEALRALYLVGAGEDLPEVERYARGEVAG
                     MSNDIKKQAAQTAEAIKSRAAPK"
     gene            complement(4720..5085)
                     /locus_tag="VGB76_22850"
     CDS             complement(4720..5085)
                     /locus_tag="VGB76_22850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101783.1"
                     /translation="MIQILDKYWPMSVGDGKATRKKKPRILAITTDCCTGCAGSPACI
                     EYCPIEACMFWVPDEDHPPFGRIEVDPGLCIGCQKCISKGPEGAFLDGCPWDAIEMVP
                     TEDWEAAHGITLPDAPAGR"
     gene            5260..>5780
                     /locus_tag="VGB76_22855"
     CDS             5260..>5780
                     /locus_tag="VGB76_22855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101784.1"
                     /translation="MNTVAGIFQSRSDAERAIENLRTTVGLPDENLNLLTPAAAEAQT
                     ETDVQTSDTEQPGMGNALGGLVGGAMGAAGGMTLGAAAASLLVPGVGPIMAAGIVGAA
                     LFGAGGAAAGMAAGGALEGNIAQGLPHDELFVYEDALRQGRTVLIVQLEDNGQVYSVQ
                     GALSEAGAESIDAA"
BASE COUNT         1094 a         1787 c         1699 g         1200 t
ORIGIN      
        1 ctcgtcctcg gccagacctc gctcgacctc ttcatccacg acgggtattt cgtgctcgca
       61 cactttcatc tcgtgatggg cgtggcggcc atcttcggca tgttcgcggg cgtctatttc
      121 tggttcccga aaatgttcgg gcgcatgatg agcgagcggc tgggacagtt gcatttctgg
      181 ccgaccttca tcggcgtcta cggcatcttc gtgccgatgc acattatggg catcgtcggc
      241 atgccgcgtc gttactcgtc gtttcaggag tacgacttcc tgcgcccgct cgacccgctc
      301 gtgctgctgg tgacgattgc cgccatcgtc acggcggtcg cgcagtttat ctttctcttc
      361 aatttcatct acagcatctt caagggcgag cgcgcgagcg gcaacccctg ggaagcgacg
      421 acgctcgaat gggtgacgcc cacgacgccc ccgccgcacg acaacttcgc aggccatatc
      481 ccgaccgtct atcgtggcgc gtatgaattc gccgtgcccg gtgcgcccga agactacgtg
      541 ttgcagacga cgcccgatgc ggaggatgag aacatcaccc cggctgagca aacattagcg
      601 caaggaacac agggaagttc aggtaatggt cacaacggtc accactaaaa attcgggcag
      661 cgtctcgacc acaacgaagc cgctcaacgt gggcgggacg ggacgacgcc ccggaggcaa
      721 cggctcgcgc ggcgggccgc agccgccaaa cggcaacgac ggcgaacgtt acgggcgcgc
      781 gccccgtgat gccacgccgc agcagtatcg catcggtatg tggctggctc tgtccgcgat
      841 cctgatgatg ttcgcggcct tgacgagcgc gtacgtgttt cgctccacgc ggacgcagca
      901 ggagtggcag gtgttcgccg tgccgcagat gctctgggtg agcacgctta tgattcttgc
      961 cagcagtgcg acctttgaag ccgcgaggcg cgcggagcag cgcgggcggc atggattata
     1021 tcgaaactgg ctgctcgttt cgctgttgct cggcttcggt ttcctcgcct cgcaattgct
     1081 ggcctggcgc gaactcgtcg ggcagggaat ttatctcgcc agcaacccgc acagttcgtt
     1141 cttctatttg ctgaccgggc tgcacgcgct ccacctgctc ggcggcatac tcggtttgag
     1201 ctatctcgcc attagcgcga gacggaaaac cgccccgccc ggcgatacgc ttgacgtgcc
     1261 ggacgcgggc aatagagaca ggaagcggcg cgcgagcgtc gatgctatcg gcctctactg
     1321 gcatttcatg gacggcttgt gggtctattt gttcgcactc cttttcctct ggaggtaaga
     1381 gttgagcaca catgcgcaga cagagcattt gatcgcggat gattggagcg gcgggacatt
     1441 cccgtatcac gtcggccaca agaaactcgg catgtggctc ttcatcgtct cggacgcgtt
     1501 aaccttctcc gcgctcctca tcggctacag ctacatgcgc gtctccagcg accagtggcc
     1561 gactcctttc gccttctacc cgagcatcat cttttcaacc gtgatgacgc tctgcctgtt
     1621 ggcttcgagt tttacgatgg tgatggccgt ggccgcctcc gcccggcgcg accgccggaa
     1681 ggcgcgtcgc tggatcggcg cgacgatact cggcggcctc acgttcctcg tgctgcacat
     1741 ctatgagtgg ctgcacctga tcaacgacta cggcctcaaa cccttcacgc tccccgccgc
     1801 atggatcgaa cgctggccgc aagcctcgcc gctcttcggc gcaaccttct tcgccatcac
     1861 cggactgcac atgttccacg tcctgaccgg cgtgatctat ctcggcgtca tcgctctgcg
     1921 catccgtcgc aagacacacg aagacgttga gatcagcggc ctctactggc acttcgtcga
     1981 tctcgtctgg atgttcgtct tcccgttgat ctatttgctg tcggtggatt tccatccggt
     2041 gcactaggag tgcgggcacg caaactttgc aaaagtgagg tggattaaag acacgatgag
     2101 cacacgtttc ggagcactcg gcaatttgat gaagaagcag gacttgatcc tgaacggggt
     2161 ctgcctgctc ttcgcgacgg gcttcctcgc catggctctc tcgaacgtgc gttgggcggg
     2221 aaacttcttt tccatcgaca gcctcttctt cacgctcgtg agtctcaccc tcgccggaat
     2281 ctttctcgcc attcccctgt actgggctta tgagacgggc atgctgcgcg acttcttcgc
     2341 cgccgaagac gagacgacgc tcgcggcggt agatgccgcc cccgtgcact ttgagggcac
     2401 gacgaaactc ttcctttcga tcctcgtcta cctgctggtg ctaacggcca tcgaagtcgc
     2461 gctggcctac gttcaggtgc cgctcgcttt gatgttgacg ctcctcgtcg gcctgtcgct
     2521 cgtcaaagcc gcgctcattc tcgcttactt catgcacctg cgttacgaac gcctgagtct
     2581 cgtctacacg ttgatcccga tgctcgtgat ctgcatctgc ctcctcttca ttttcttccc
     2641 tgacagtatc aggttactca acctgcgtat ttacaaataa ttttttgcgg ccatgaagat
     2701 cgaacccgca gccagaactt tactcttact cgcgctcgtc tacgcggcgt gggcagggtt
     2761 ctttgccact gacgcgctcg cgcagtgtgc gatgtgcaaa gcctctgcgg ccaatctcga
     2821 cagcatctcg gcgcggcaac tcaatatctc gacgctcatc ctgctctgcc cgccggtcgc
     2881 gatcttttgc gcgatcttcg tcgtcgccta caaacaccgc aatccgcccg acgacgaatc
     2941 ctgagtccaa agcgaaacga agatggcaaa cgacaacgaa gccctagccc tcaataacga
     3001 ccatcagagc atacgcgggc gcgcctgccc gcagtgcggc ggcaagatta tcccttcaga
     3061 ccgcgcggtt tacgaaagca aaaacgatcc ggccagcgtc ttcccgctct ggcagtgcga
     3121 gcgttgcggt tacgagcaga tgagcgagag gcagtccgcg ccgaagaagg ccgcgcccca
     3181 cgcagcggcg aaaaaaagag ccgagtaatg aaggcgacgc acagcgttgc tcttctaagc
     3241 gaaccgtcgc cgtttcgtgc tcgcctctac ttcgttcatt tcaagcgata cgggcaatgt
     3301 gcagcgcgat gctataatcg gggcatcttt agcttctccg aaatttcatg gattattcaa
     3361 agctgccgca catcaacgcc ctgctcaatg cgacgagcgg gctgctcctc ctcgccggtt
     3421 tttatttcat cagccgccgc cggatcacgg cgcacttgcg ctgcatgaca gccgcgctcg
     3481 ccgtctcctg tatctttctc gcgtcctatc tggtctatca ctacaatcac ggctcgacgc
     3541 gttttacggg gcagggctac gtcaggccgg tctatttctt catcctgctg acgcacacga
     3601 tcctcgcggc catcatcgtt ccgctcatcg ccgtaacgct caggcgcgcg tggcgcggcg
     3661 acttcgggcg gcaccgcaag atcgcgcgtt ggacttttcc gctctggctc tacgtctccg
     3721 tgacgggcgt tatcgtctat ctgatgcttt atcaactcta cccctcgcgc tagcgtgtgc
     3781 agccgtagcg gcagcgacgc gcattcggaa gaagatggac ggggataatt caaacaatca
     3841 tcacacgcgc aagcatcctg caccggacac ggcggacgcg cctgctgcga cgactgcgcg
     3901 ccgtcgctcg ccgtggccgc tcgccgtcgt cgccgcgctc tttattatcc tgccgttcct
     3961 ctcctggtat ggcacgacct tctggcgtcc gctgaacgac gaggagatcg aaaaatatct
     4021 cgccgatacg gagaagccgc gccacgtgca gcatgcgctc gaacagatag acaaacgcat
     4081 cattgcggga gatggggagg cgaggaaatg gtatccgcgt gtggtcgggg tggcgggaaa
     4141 tccgttgcct gacttgcgca tgaccgccgc gtgggtgatg ggcgacgata acaagtcgga
     4201 agagtttcac gtcgcgctga agtctctgct tgccgatagc gagcctgcgg tgcgcaggat
     4261 ggcggcgttg tcactctcac gcttcaacga tgggagcggg cgcgcggagt tgatctcgat
     4321 gttgcgaccc tttagcgtca aactaccgca ggcgggaacg ctgctcacag tgctgccggt
     4381 gaattcaccc gtcaccagag aagcgatgct ggcgcgcgtg agagatgaaa agggagccgt
     4441 gcaggaagtt cgcgctcccg tcgcgggcaa gatcgagcgc gtatctgtga cggacggaac
     4501 gagcgtggcg gcgggaactg aactgctcgt gctcgcgccc gacggcaaaa acgtgtggga
     4561 ggctttgaga gctttgtacc tggtgggcgc gggcgaagat ttgccggagg ttgagcgtta
     4621 cgcgcgtgga gaggtggcgg gaatgtcgaa cgacatcaaa aaacaggcgg ctcagacagc
     4681 ggaagccatc aagagccgcg ctgctccaaa gtaagatttt tatcgtccgg cgggcgcgtc
     4741 cggaagagtg atgccgtgtg cggcttccca gtcttcggtc ggaaccatct caatcgcatc
     4801 ccacgggcag ccgtcgagaa acgcgccctc cggtcctttg ctgatacact tttgacaacc
     4861 gatgcagagt cccggatcaa cttcaatacg accgaacggc gggtggtctt cgtccggcac
     4921 ccagaacatg cacgcttcaa tcgggcaata ctcgatacac gcgggcgaac ccgcacagcc
     4981 cgtgcagcaa tcggtggtga tggcgaggat gcgcggtttc tttttgcgcg tggctttgcc
     5041 gtcgcccacg gacatcggcc agtatttatc caaaatctga atcattcgcg tgcgctcccc
     5101 gtgcggctct ctgaggttgg acgtgcgaga gacactttaa cacgagcaca ggagcggctc
     5161 aatcctcttt ttcgcgcgcg ccgcacggat aacttgaatg tcgcttcgta aagagataaa
     5221 actaatccac acttaaacct taaggagact tgacaagcga tgaacacagt cgccggaatc
     5281 ttccagtcgc gctctgacgc cgagcgtgcc atcgaaaacc tgcgcacgac agtcggcctc
     5341 ccggatgaga acctcaacct gctgacgccc gccgcagcgg aagcgcagac cgagacagac
     5401 gtacagacga gcgacacgga gcagccgggc atgggcaatg ccctcggcgg actcgtcggc
     5461 ggtgcgatgg gcgcggcggg cgggatgacg ctcggagccg cggcggccag tctgcttgtg
     5521 ccgggagttg gacccatcat ggccgccgga atcgtcggcg cggctctgtt cggcgcgggc
     5581 ggcgcggcgg cgggaatggc tgcgggcggc gcgctcgaag gaaacatcgc acaaggtcta
     5641 cctcacgatg aactcttcgt ctatgaagac gcgctcaggc aggggcgcac cgtcctcatc
     5701 gtccagttgg aagacaacgg acaggtctat tccgtgcaag gtgcgctaag tgaggcgggg
     5761 gcggaaagca tagacgccgc
//
LOCUS       DASWNS010000659         7007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_335326, whole genome shotgun sequence.
ACCESSION   DASWNS010000659 DASWNS010000000
VERSION     DASWNS010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335326"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3973)
                     /locus_tag="VGB76_22860"
     CDS             complement(<1..3973)
                     /locus_tag="VGB76_22860"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY0101785.1"
                     /translation="MQQQVEGFRLSPQQARLWLLREQYPVSRLAQRAIFIEGSLDIEA
                     LKRALREIVARHDILRTGFYAQTGIKMPLQVIAEDGEPEWRVVAREDFDGREASVLDE
                     LLREERSRSFSAGQQTRTRFTLVTLDGGSHLLLAGLPALCADAHTLNNLSEEISRLYP
                     SGASGAEEAGEAVQYVQFSEWQNELLESDDADEGRDFWQRQRASGADALTLPCEVKTQ
                     SQPSDGTASVEMTIPPATISKLESLASAHDTSSEICLLACWQLLLWRLSAWAEVSVNV
                     LFDGRKYEELQGAFGLFARFLPVSCRFEAGLTFAQVIRQVAQSVQEARDWQEYSISEA
                     GEELSADRSNPPVSFEFVRWGGKRQAAGVAFTTCRQHVELEPAKLKLSCVQKDGALVT
                     AFVYDASAFSSEAARQLAQQFHRLLESGLDAPHAAAGKLLLLGDEEREHLLRRLNETE
                     RAYPKEFCFHQLLEAQAARTPGAAAVACKGVQLSYTELNERANQLAHHLQRHGVRPEV
                     RVGICLERSIEMVVGLLGILKAGGAYVPLDAHIPRERLAFMIEDARLHLLLTQQSLSD
                     LPVASGTRTLCIDTEWPQIAQESAANPDSGATPGNAAYVIYTSGSTGTPKGVLIHQQG
                     LVNYLSWSLEAYRVAEGDGALVHSPVGFDLTVTGLYTPLLAGRCVTLLPEDAGVDTLS
                     DAIGAGRGFSLVKLTPAHLTALAQLLPAANAPSQAHCLVVGGEALFGEHLSFWREHAP
                     ATRIVNEYGPTETVVGCCVHTSTAEAVGSGPVPIGRPIANTQLYVLDENLSPAPTGVA
                     GELYIGGDGVARGYVNRPELTAERFLPNPFNAQPGARMYRTGDLVRYLPDGALEYIGR
                     VDNQVKIRGYRIELGDVEAALATHEGVRECVVVAREDVPGDRRLVAYLVLKQERGLMI
                     SELRAFLIEKLPEYMIPTKVVILDALPLTANGKIDRRALPHPDVSRLKLEQPYIAPRT
                     AAEEVLTNIWAEVLGLERVGIHDNYFALGGDSIRSVRVLALAKERGMEFTLQSLFRHQ
                     TVAELAGELENGETISSALDAVSPFSLISEADRLKVPEGIEDAYPLSMLQAGMLYHME
                     LLSDRPAYHNACSYEVRAHFDAAVFEEAVNRVVARHPALRTSFDLSSYSEPLQLVHRM
                     AQMPVPVDDLRHLSAKEQEEFLGGFIESEWKTLFDLSRPPLVRIHIHRRTSETFQLTL
                     AECHAVIDGWSLTSTFAEVFTNYFAYLKTGAFPEADPLPTSFRDFIYLERAALDSEEH
                     RQFWARKLDGCEPLRLPRRTTPPAANDGQRMNIQPVPISAEASESMRRLARTLSVPLK
                     SVLL"
     gene            complement(4222..>7007)
                     /locus_tag="VGB76_22865"
     CDS             complement(4222..>7007)
                     /locus_tag="VGB76_22865"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEY0101786.1"
                     /translation="TPNGKVDRRALPDPEDQRGQSGDNYSAPRTPAEELLAHIFAEVL
                     RLERVGTDESFFNLGGHSLLATQVVSRVREAFRVDVALRALFERPTVAELARHLEAQL
                     RAGKRVAAPSIKRVERNVVLPLSFAQQRLWFFEQFQPGSTTYNLLQSARLLGDLNVSA
                     LEQSLNEILRRHESLRTTFQNIKGEPAQEIAPPSTLTLPLVDLSGLPEAEREKEVERL
                     TLEELNRPFELTRGPLVRARLLKLDEHTHVAVFMMHHIVTDAWSMGVLLRELTVLYDA
                     FSQGQPSPLPELPIQYADFAHWQREWFSGEVLEEQFSYWKERLGGELPVLSLPTDRPR
                     PDVWSYRGAKHPVQFSKSLTEQLKTLGSRQGATLFMTLLAAFQMLLHYYTGEEDIVVG
                     TDDANRGHVETESLIGFFINQLALRTDLSGNPSFTELLARVREATLGAYANHDLPFDK
                     IIEKLRPERSLSHAPLFQVKFVLQNTPAEELELRGLKLVPVQTDYVAAKFDLTPLLWE
                     KPDGLRGWIEYSSDLFNAATVARIARQFEVVLNSINARPDITLAQLRELLSEDDRQRR
                     LREQKEREATQRNRFKTIKPKAVSLPEQTLLVQTTELQPGERLPLVIQPATNKLDLEE
                     WTKANLSFIEEKLQRHGALLFRGFAVDSASAFEKFAHAICPRLFDENGEHQRDSVSGK
                     VYTPVAYPANKLLLWHNENSFNNSWPMKIWFCCASPAAEGGETPLVDSRRVFEMLDPK
                     IRERFSQKQVMYVRNYKEGLGQSWQAIMRASTRAQAEEYCRKNFMEFEWKEGNNLVTR
                     CVRPAVSRHPRTGEMVWFNQAQHWHPSCLDAETRDSLRAVFGEDDLPRGCYYGDGSAI
                     EDSVMEEILEVYRQLEVSFKWEARDVVMLDNMLTAHGRNPYVGERRLYVAMGEMTSYA
                     DIPSPEAANLLPV"
BASE COUNT         1355 a         2169 c         2095 g         1388 t
ORIGIN      
        1 cgagtagaac actcttgagg ggcacggaga gcgtgcgcgc caaacgtcgc atactctcag
       61 acgcctctgc cgagatgggc acgggttgaa tgttcatccg ctggccgtcg ttggcggcgg
      121 gcggcgtggt gcggcgcggg aggcgcagag gctcgcagcc gtctaacttc cgcgcccaga
      181 attgccggtg ttcctcggag tcgagggcgg cgcgctccag gtagataaaa tcgcggaacg
      241 aagtcgggag cgggtctgct tcggggaaag ctcccgtctt cagataggcg aaatagttcg
      301 tgaagacttc ggcaaaagtc gaggtcagac tccagccgtc aatcacggcg tggcattcgg
      361 ccagcgtcag ttgaaaggtt tcgctggtgc ggcgatgtat gtggattcga accagcggcg
      421 ggcgcgacag atcaaagagc gtcttccact cgctctcgat aaatccaccc aagaactctt
      481 cctgttcttt agcggacagg tggcgcaggt cgtccacggg cacaggcatc tgcgccatcc
      541 ggtgtacaag ttggagcggt tcgctataac tggacagatc gaatgaggtg cggagcgcgg
      601 gatggcgtgc gacgacacgg ttgacggctt cttcaaagac tgcggcgtcg aagtgtgcgc
      661 gaacctcata gctacaggcg ttatgataag caggacggtc ggagagcagt tccatgtgat
      721 agagcattcc ggcctgaagc atgctcaacg gataggcgtc ttcgatgccc tccggaactt
      781 tcagtcgatc cgcttccgag atcaggctga aagggctgac ggcatccaga gcactcgaaa
      841 tcgtttcgcc gttttcgagt tcaccggcca gttcggcgac ggtctggtga cggaagaggc
      901 tctggagagt gaattccatc ccccgctctt tagcgagggc gagcacacgc acgctgcgga
      961 tcgagtcgcc tccgagcgca aagtaattgt cgtgaatgcc gacccgttca aggccgagca
     1021 cctcagccca gatgttcgtc aacacctcct cggcggcggt gcgcggcgcg atgtaaggct
     1081 gctcaagttt cagacgcgaa acgtccgggt gcggtaaggc gcggcggtct atcttgccgt
     1141 tggcggtcag aggcagcgca tccagaatca cgaccttcgt ggggatcatg tattccggca
     1201 gcttctcgat gaggaatgcg cgcagttcgc tgatcatcaa tccgcgctcc tgcttcaaca
     1261 ccagataggc caccaaacgt ctatcaccgg gcacatcctc gcgcgccacg accacgcact
     1321 cgcgcacgcc ttcatgcgtc gccagcgcgg cctccacatc tcccaactca atgcggtagc
     1381 ctctgatttt cacctgatta tcaacccgcc cgatgtattc gagcgcgccg tccggcaggt
     1441 atcgcacgag gtcgcccgtg cgatacattc tcgcaccggg ttgagcgttg aaggggttcg
     1501 gcagaaatcg ctcggcggtc aactccgggc ggttcacgta accgcgcgca acgccgtcgc
     1561 cgccgatata cagttcaccg gccacgcccg tcggagcggg cgacaagttc tcgtccagca
     1621 cgtacaactg cgtgttggcg attggtcttc cgatgggcac cggaccactc ccgaccgctt
     1681 ccgccgtgga cgtatgcacg cagcatccca cgaccgtctc cgtcgggccg tactcgttaa
     1741 caattctggt tgccggagcg tgctctcgcc agaaagagag atgttcgccg aacagtgctt
     1801 cgccgccgac gaccagacag tgagcctgtg aaggggcgtt cgcggcgggc aggagttggg
     1861 cgagcgccgt gagatgtgca ggcgtcagct tgaccagact gaaaccccga cccgcgccga
     1921 tggcgtcgct cagcgtatcg acgcccgcgt cctccggcag caacgtcacg catcgtccgg
     1981 ccaacaaagg cgtgtagagt ccggtgacgg taagatcgaa tccgaccggc gagtggacga
     2041 gcgcgccatc tccctcggcc acgcggtaag cctccagact ccaactcaga taattgacca
     2101 acccctgttg atgaatcaac acgcccttcg gcgtgcccgt cgagccggag gtataaatga
     2161 cgtacgccgc gttgccgggc gtcgcgccgg agtccggatt agcggcactc tcctgcgcga
     2221 tctgcggcca ttcggtatca atgcagagcg tgcgtgtgcc ggaggcaacg ggcaggtcgc
     2281 tcagactttg ctgtgtcagc aggagatgta aacgcgcatc ttcgatcatg aaggcgagtc
     2341 gctcgcgcgg gatatgcgca tccagtggaa cgtacgcgcc gcccgccttg aggatgccga
     2401 gcagaccgac gaccatctct atcgaacgtt caaggcagat gccgacacgc acctccggcc
     2461 tgacgccgtg ccgctgtaaa tgatgcgcta actgattcgc gcgctcgttc agttccgtat
     2521 aactaagctg cacgcccttg caggcgactg ccgccgcacc gggcgtgcgc gccgcctgtg
     2581 cctctaacag ttgatggaag cagaactctt tcgggtaggc gcgctctgtt tcgttgagcc
     2641 gtcggaggag gtgttcgcgt tcctcgtcgc cgagcaggag cagtttaccg gcggcggcgt
     2701 gcggggcgtc gaggccgctc tcaagcagcc tgtgaaactg ttgtgccagt tgccgcgcgg
     2761 cctcggatga gaaggcggac gcgtcgtaga cgaacgccgt cacgagcgcg ccatcttttt
     2821 ggacgcagga gagcttgagt ttcgcaggct caagctctac atgctgacga caggtggtga
     2881 acgcgactcc ggcggcctgt cgcttcccgc cccagcgcac aaactcaaaa ctaacgggcg
     2941 ggttcgagcg gtctgcgctc aactcttcgc ccgcttccga gatggaatac tcctgccaat
     3001 cgcgcgcctc ctgcaccgac tgtgcgacct gccggatcac ctgcgcgaaa gttagccccg
     3061 cttcaaaacg gcaactcacg ggcagaaacc gcgcgaacag accgaacgcc ccctgcaact
     3121 cctcatactt ccgtccgtcg aagagaacgt tgacggacac ctccgcccac gcgctcaaac
     3181 gccacagcag cagctgccaa catgccagca gacatatctc gctcgaagtg tcgtgcgcgc
     3241 tcgccagcga ctccagtttg gagatcgttg cgggcgggat cgtcatctcg acggaagccg
     3301 tcccgtcgga cggctgcgac tgggttttga cttcgcaggg gagagtcaaa gcgtctgcgc
     3361 cggaggcgcg ttgccgctgc cagaagtcgc ggccttcgtc tgcgtcgtcg ctctcaagca
     3421 actcgttctg ccactcggag aattgaacgt actgcaccgc ctcgcccgct tcttccgcgc
     3481 cggacgcgcc ggaaggataa agacggctga tctcttcgga caggttgttc agggtgtgcg
     3541 cgtccgcgca gagtgcgggt agcccggcga gcaggaggtg gctgccgccg tccaaagtca
     3601 ccagagtaaa acgcgtgcgg gtctgctgcc cggcactgaa agagcggctt cgttcttcgc
     3661 gcaggagttc gtcgaggaca gacgcttccc gcccgtcgaa gtcttcacgc gcgacgacgc
     3721 gccattccgg ttcgccatct tccgcgatca cctgaagcgg cattttgatg cccgtctggg
     3781 cgtagaaacc cgtccgcagg atgtcgtgcc gcgccacgat ctctcgcaag gctcgcttga
     3841 gtgcttcgat gtcgagactt ccctcaataa aaatcgcgcg ctgggcgagg cggctcaccg
     3901 ggtactgctc tctcagcagc cagagccgcg cttgctgggg ggaaagacga aagccttcaa
     3961 cctgctgttg cattgatcac tccttaaccg tttaacacct gccggggtta aatttttaaa
     4021 tcaggtaagc caggacgagc acttccggtg cggggaaaca aattatcgtg ccagtaaaat
     4081 atgaagccgg atcgcgcggc gacgactcgc ggaagttgcc gccgcatgaa ccgaaatctt
     4141 ttaagcttgc atcattaagc accaattgac tgacgccgta gcgaggccgg gaggcaaagg
     4201 caacgccggt cgcccgtgcg ctcaaaccgg cagcaggttc gcggcttcgg gcgaagggat
     4261 gtccgcatag ctggtcatct cgcccatcgc cacgtacaac ctgcgctctc cgacgtaagg
     4321 attgcgcccg tgcgccgtga gcatgttgtc gagcataaca acgtcgcgcg cctcccactt
     4381 gaagctcacc tccaactgcc ggtagacctc cagaatctct tccatcaccg aatcctcaat
     4441 cgcgctgccg tcgccgtagt aacaaccgcg cggcaagtcg tcctcgccaa agaccgctct
     4501 cagcgaatcg cgcgtctcgg cgtccagaca ggagggatgc cagtgctgcg cctgattgaa
     4561 ccagaccatc tctcccgtgc gtgggtgtct ggacaccgcc ggacgaacac agcgcgtcac
     4621 cagattgttt ccttccttcc attcaaattc catgaagttc ttccggcagt actcctcggc
     4681 ctgcgccctc gtcgaggcgc gcatgatcgc ctgccaactc tgccccaacc cttccttgta
     4741 gttgcgcaca tacatcacct gcttttggct gaaccgctcc ctgatcttcg gatcgagcat
     4801 ttcaaatact cgccgactgt caaccagcgg cgtctcgccc ccctccgccg cgggactcgc
     4861 gcagcaaaac cagatcttca tcggccagga gttgttgaag gagttttcgt tgtgccacag
     4921 gagcagtttg ttcgccggat aggccacggg cgtgtagacc ttcccgctca cgctatcgcg
     4981 ctggtgctcg ccgttctcgt cgaacagacg ggggcagatg gcgtgcgcga atttttcgaa
     5041 ggcggaggcc gaatcgacgg cgaaaccgcg aaacaggagc gcgccgtgcc gctgcaattt
     5101 ttcttcaatg aaactgagat tcgccttcgt ccactcttcg agatcgagtt tgttcgtcgc
     5161 gggctgaatc accagcggca gacgctcgcc gggttgcagt tccgtggtct gcacgaggag
     5221 cgtttgttcc ggcaggctga cggccttcgg cttgatcgtt ttgaacctgt ttcgctgcgt
     5281 cgcttcgcgt tctttctgct cgcgcaggcg tcgctgcctg tcatcttccg agagcagttc
     5341 gcggagttgc gcgagagtga tgtcgggtcg ggcgttgatg ctgttcaaga cgacttcaaa
     5401 ttggcgcgcg atgcgtgcca ccgtcgcggc gttgaacagg tcggagctat attcgatcca
     5461 gccgcgcagc ccgtcgggct tttcccacag gagcggcgtg aggtcgaatt tggccgcgac
     5521 ataatccgtc tgcacgggca ccagtttcag cccgcgcagt tccaattcct ctgcgggcgt
     5581 attttgcagc acgaacttga cctgaaagag cggcgcgtga gagaggctcc gctcaggacg
     5641 aagtttttcg atgatcttat cgaaaggcag gtcgtgattc gcataagctc cgagggtcgc
     5701 ttcccgcacg cgcgccagca actccgtaaa gctcgggttg ccggagaggt ctgtccgcaa
     5761 cgccagttga ttgatgaaga acccgatcag agattccgtc tcgacgtggc cgcgattggc
     5821 atcgtccgtg cccaccacga tgtcttcctc gcccgtgtag tagtgcagca acatctggaa
     5881 ggccgcgagc agagtcataa acagggtagc gccttgccgg ctgccgagtg tcttgagttg
     5941 ctcggtcaga ctctttgaga actgcacggg atgttttgcg ccccgatagc tccacacgtc
     6001 aggccgtggc cggtctgtcg gcagagacaa gacggggagt tcgccgccaa gccgctcctt
     6061 ccagtaagag aactgctcct ccagcacctc cccgctgaac cattcgcgtt gccagtgggc
     6121 gaaatccgca tattgaatcg gcagttccgg cagaggcgac ggctgcccct gactgaaagc
     6181 atcatacaaa accgttaact cccgcagcag gactcccatc gaccacgcat cggtgacgat
     6241 gtgatgcatc atgaagacgg cgacatgtgt gtgctcgtcg agtttcagca ggcgtgcgcg
     6301 cacgagtggt ccacgagtta gctcgaaagg ccggtttaac tcttccagag tcagacgctc
     6361 aacttccttc tcccgttcgg cttcgggcaa cccgctcaaa tcaaccagcg gcaatgtcag
     6421 cgttgacgga ggcgcgatct cctgcgccgg ttctcccttt atgttctgaa aggtcgtgcg
     6481 cagcgattcg tggcgtcgca ggatttcgtt caaagactgt tcaagcgcgg agacgttcaa
     6541 atccccgagc aggcgcgccg attgaagcag gttgtaagtg gtgcttcccg gttggaactg
     6601 ttcgaaaaac cagagacgct gctgcgcaaa cgagagcggc aatacgacgt tgcgctcgac
     6661 tctcttgatg gacggcgcgg ccaccctctt tcccgctctc aactgcgctt cgagatggcg
     6721 tgccagttcg gccaccgtgg ggcgttcgaa tagggcgcgc aaggcaacgt ccacgcgaaa
     6781 agcctcccgc actcttgaaa caacctgcgt agcgagcagc gagtggcctc ccaaattgaa
     6841 gaagctctca tcagtgccga ccctctccag cctcaacact tcggcgaaga tgtgcgcgag
     6901 caattcctcg gcaggcgttc gcggcgcaga gtagttgtcg ccgctctgcc cacgctggtc
     6961 ttcggggtca ggcagcgcac ggcggtctac cttgccgttc ggcgtca
//
LOCUS       DASWNS010000660         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_207539, whole genome shotgun sequence.
ACCESSION   DASWNS010000660 DASWNS010000000
VERSION     DASWNS010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207539"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2230
                     /locus_tag="VGB76_22870"
     CDS             <1..2230
                     /locus_tag="VGB76_22870"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101787.1"
                     /translation="TSGRVAFEVELAGQPSHDPNPIALTVRDTGGNVVAQTGGGGITL
                     KAPRTFSVQINSPQLWSPQRPTLYTVEVSLNDGAERDVVRERFGFRSFAARDGKFYLN
                     GEPYYMIAALDQDFYPETIYSSPPEEYVRDMMLKAKQLGLNLLRCHIKVPDPVYLKVA
                     DEVGMLVWYEVPSWNDFNHFSPQAAARGEKVFQEMTERDWNHPSIVIQSIINEGWGTD
                     LSREAAHRKWLRAAFERAKRQTAPLARLIVDNSPCCNNFHLKTDIEDNHRYNSIPDEH
                     TAFDRWVADFAARPKWNFSPHGDAEPTGREPLVLSEFGNWGLPKLPAELPWWFERGFG
                     DRAVTRPGGVLERFREFGFARLFAGYDALAEATQWHQFVSLKHEIEEIRRHASIQGYV
                     ITEFTDINWEANGLLDMWRNPKVYAADLARLQQPDLLVTRTTKKNYTGGEYISFETYL
                     SHYSARDLLGAKFSWRTEGGHEHSTLVLDKPIERASVSLLSRHGFDAPEVGLPRRQRI
                     LFELRDKNDQLITENSAEVFIYPKPEGDEAVPRLLNFYDPKNSQPSLKSALPAAGRYG
                     VNVDGRQRPGTLLVATVFDDFVREHLRGGGRAVILADAKDALPPGSNLKITPRAGSDL
                     DGNWVTNFNWARTDAQPFREVAFTKILGFEAARVVPRHVIEGVGGADYADVHAGIFYG
                     WLNNNRALALQARVGTGGGRVFLTTFRFEEYGRDPYSTRLLDAIIGYTGSQAFAPKLE
                     MK"
     gene            2303..2488
                     /locus_tag="VGB76_22875"
     CDS             2303..2488
                     /locus_tag="VGB76_22875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101788.1"
                     /translation="MQRRQITLEDGRYLVFYTFDEMKTTEAPAAATSATTGDAPDASR
                     PDAEPSPVPQATEEPRV"
     gene            2481..>2677
                     /locus_tag="VGB76_22880"
     CDS             2481..>2677
                     /locus_tag="VGB76_22880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101789.1"
                     /translation="MSELRWNPLMGEWVATATHRQTRTFLPPADFCPLCPTKPGGFPT
                     EIPEPTYDIVAFENRFPSLQK"
BASE COUNT          553 a          853 c          797 g          474 t
ORIGIN      
        1 caccagcggg cgggtcgcgt tcgaagtgga actggccggg cagccatcgc acgatcccaa
       61 cccgattgcc ttgacggtgc gcgacacggg cggcaacgtc gtggcacaga cgggcggcgg
      121 gggcatcacg ctcaaagccc cacgtacttt cagcgtccag ataaactcgc cgcaactttg
      181 gagtccgcaa agaccgacgc tctacacggt tgaagtaagt ctgaacgacg gcgcagagag
      241 agatgtcgtg cgcgaacgct tcggcttccg ctccttcgcg gcgcgcgacg gcaagtttta
      301 tttgaacggc gaaccgtact acatgatcgc cgcgctcgat caggacttct acccggagac
      361 gatctattcc tcgccgcccg aagagtacgt gcgcgacatg atgttgaagg ccaagcaact
      421 cggcctgaac ctgctgcgct gccacatcaa agtccccgac cccgtctacc tgaaagtggc
      481 cgatgaagtc gggatgctcg tctggtacga agtgccttcg tggaacgact tcaaccactt
      541 ttcgccgcag gccgccgcac gcggggagaa ggtttttcag gagatgaccg agcgcgactg
      601 gaaccatcct tcaatcgtca tccagagcat catcaacgaa ggctggggca cggacttgag
      661 cagagaggcc gcacatcgca agtggctgcg cgccgccttc gaacgcgcga aacgacagac
      721 cgcgccgctc gcgcgtctca tcgtggacaa cagcccgtgt tgtaataatt ttcatctcaa
      781 gaccgacatc gaagacaacc atcgttacaa ctcgatcccg gatgaacaca cggccttcga
      841 ccgctgggtg gccgatttcg ccgcgcgccc gaaatggaat ttcagcccgc acggcgatgc
      901 ggagccgacg gggcgggagc cgctcgtcct ctccgagttc ggcaactggg gcttgccgaa
      961 gttgcccgcg gagttgccgt ggtggttcga gcgcggcttt ggcgaccgcg ccgtcacgcg
     1021 tcccggcgga gtgctggaac gcttccgaga gttcgggttt gcgcgtctct tcgcgggcta
     1081 cgatgcgctc gccgaagcga cgcagtggca tcaattcgtc tcgctcaaac atgagatcga
     1141 agagatacgc cgccacgcct ccatacaggg ctacgtcatc acggagttta cggacatcaa
     1201 ctgggaagcg aacgggctgt tggacatgtg gcgcaacccg aaagtctatg cggcagacct
     1261 cgcgcgcctt cagcagcccg atctcctcgt cacgcgcacg acgaagaaga attacaccgg
     1321 cggcgagtac atctctttcg agacgtacct atctcattac agcgcgcgcg atctccttgg
     1381 cgcaaagttt tcgtggcgca cggagggggg acatgagcac agcacgctcg tcctcgataa
     1441 accaatcgag cgggcgagcg tctctttgct ctcgcgtcat ggcttcgacg cgcccgaagt
     1501 cgggcttccg cgtcgccagc gcatcctctt cgagttgcgc gataagaacg atcaattgat
     1561 cacggagaac agcgcggaag tcttcatcta cccgaagccg gagggcgacg aagccgttcc
     1621 ccgcctgctt aatttctacg acccgaagaa cagccagccg tctctgaaaa gcgcgcttcc
     1681 ggcagccgga cgttacggtg tcaacgtcga tggcaggcag cgtccgggta cactgctcgt
     1741 cgctaccgtg ttcgacgatt ttgtcagaga gcacctgcgc ggcggcgggc gcgcagtcat
     1801 tctcgccgac gcgaaagacg ccctgccacc cggctcgaat ctcaagatca cgccgcgcgc
     1861 aggctcagac ctcgacggca actgggtgac caacttcaac tgggcgcgca ccgacgcaca
     1921 gcctttccgc gaagtcgcgt tcacgaagat tctcggcttc gaagcggcgc gcgtcgtgcc
     1981 gcgccacgtc atcgaaggcg tgggcggcgc ggattacgcg gacgtacacg cggggatttt
     2041 ttacggctgg ttgaataaca accgcgcgct cgccctgcaa gcgcgcgtgg gcacgggcgg
     2101 cggtcgcgtc tttctcacga cctttcgctt cgaagagtat ggccgcgacc cttacagcac
     2161 gcgcctgctc gacgccatca tcggctacac gggcagtcag gctttcgcgc cgaagctgga
     2221 aatgaaatag tttgcggagt cggttaaaat cgccgcgctt caatttgcgc gacaacaact
     2281 cagtctaaac aacgggggcc gcatgcaacg caggcagatc acgctcgaag acggacgcta
     2341 tctcgtcttc tacacgttcg acgaaatgaa gacgacggaa gcaccggcgg cggcaacttc
     2401 cgcaacgacg ggcgacgcac cggacgcttc gaggccggac gccgaaccct cgcccgtgcc
     2461 gcaagcgacg gaggaaccac gtgtctgaac ttcgctggaa tccactaatg ggcgaatggg
     2521 tggcgacggc cacacacagg cagacgcgca cctttctgcc gcccgccgat ttctgcccgc
     2581 tctgcccgac gaagcccggc ggctttccca cagagattcc cgaaccgact tacgatatcg
     2641 tcgccttcga gaatcgcttt ccgagtttgc agaagga
//
LOCUS       DASWNS010000661         3272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_77511, whole genome shotgun sequence.
ACCESSION   DASWNS010000661 DASWNS010000000
VERSION     DASWNS010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77511"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VGB76_22885"
     CDS             complement(<1..701)
                     /locus_tag="VGB76_22885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY0101790.1"
                     /translation="MHPFKSIPTRLIFFACVLSVFACPAAPAQEKEKEKFDVKANYEK
                     REYRIPVRDGVKLFTSVYLPKDASRKYPIMFSRTPYTVAPYGEDKYKGLLGPSEHFMR
                     EGYIFVYQDVRGKFLSEGEYMDVRPYKPQKSSPTDIDESTDTYDTIDWLIKNIPNHNG
                     RVGMWGISYPGFYTSMGLLGAHPALRAASPQAPIADWFIGDDWHHNGTLFTAHTLNFF
                     VSFGLPRPKPTTEWA"
     gene            944..1906
                     /locus_tag="VGB76_22890"
     CDS             944..1906
                     /locus_tag="VGB76_22890"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0101791.1"
                     /translation="MSPSWQARCLSASIRWLVRRRRWGRDEHAVAQRARRLFGAPMPY
                     RWLRTLGLKVRQVNGGGGNGGGVRGEWIEPDAEGGDGGVIFYIHGGGYVSCSAATHRP
                     ITSSLARLTGRRVFSLNYRLAPEHRYPAALDDAFAAYEWLLTHEKIAPGAISFAGDSA
                     GGGLVLACLLRARDAGLPLPACAVCFSPWTDLAGAGESQHFNDGLCAMFYTENNAEFA
                     AAYLGDASPRDPYASPAYAEFAGLPPLLLQVGSTELLLDDARRVHRKIQAAKGTSRLE
                     IYEDVAHCWQMLDGFVPEARAALRQAASFILAPLSSANGDAPKS"
     gene            2138..2743
                     /locus_tag="VGB76_22895"
     CDS             2138..2743
                     /locus_tag="VGB76_22895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101792.1"
                     /translation="MRSSKFSASVAALVFCVLCAPLPLVAQQTAGQQPDPLEALSVKE
                     REIVVAFQERVKDYAKLRESIEAKMPKLAKESTPEEIDAHKKSFEERVRAARTDAKPG
                     QMFTPDIANFIRATIRSEFKGTERKELRASVLEADTKGVPLRVNYTYPETKELVEMPP
                     TLLLKLPNLPKQLRYRFVGRNMLLVDRENGLIVDYMLNALP"
     gene            2772..>3272
                     /locus_tag="VGB76_22900"
     CDS             2772..>3272
                     /locus_tag="VGB76_22900"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0101793.1"
                     /translation="MKRNLASLLTLAALFFGGNFIKPALGQNPSGQATGVRVAASTAA
                     AATTATPPVRLTLPVKDGSVRFMVVGDTGTGTQQQQELADVMLRYRSVFPFEFALLMG
                     DNMYGGEKAEDFKKKFEDIYKPLLDGKVKFYASLGNHDESNQRFYDLFNMNGEEYYRF
                     TKGNVAF"
BASE COUNT          631 a          951 c         1046 g          644 t
ORIGIN      
        1 tcagcccact cggtcgtggg cttcgggcgg ggcaggccga acgagacgaa gaagttcaaa
       61 gtgtgcgcgg taaagagcgt cccgttgtga tgccagtcgt cgccgatgaa ccagtcggcg
      121 atgggagctt gcggcgaggc ggcacgcagc gcggggtgcg cgccgagcag ccccatcgaa
      181 gtgtagaagc cgggataaga gatgccccac atgccgacgc gcccgttgtg attcgggatg
      241 tttttaatga gccaatcgat ggtgtcgtaa gtgtccgtgc tctcgtcaat gtcggtgggg
      301 ctgctctttt gcggcttgta ggggcgcacg tccatgtact cgccttcgga cagaaacttt
      361 ccgcgcacgt cctgatagac gaagatgtag ccctcgcgca tgaaatgttc ggaaggaccc
      421 agcagcccct tgtatttatc ttcgccgtaa ggcgcgacgg tgtagggcgt gcggctgaac
      481 atgatcgggt acttgcgcga ggcgtccttc gggagataga ccgaggtgaa caacttgacg
      541 ccgtcgcgca cgggaatgcg atactcgcgc ttctcgtagt tggccttcac gtcaaacttc
      601 tccttctcct tctcctgcgc gggcgcagca ggacaggcga agacggagag gacgcaggcg
      661 aaaaagatga ggcgtgtcgg gatggatttg aacgggtgca tagagagtct cctcgctagg
      721 tgtcaggctg gcggcgggag tcgccaggga tcaacttccg tttcgtcaat gcaaattcga
      781 tgacgagagt tttcgacagc cgccgcgacg cgagaagcag ccgccggagc agtgaacaag
      841 tggtgccgcg cattgtagac gcattaggcg gcgcgcgcta gagggctttg aaaattagcg
      901 ttatgatgag gcgtttcgac ggcagcaatt ttacggcaac tctatgagtc caagctggca
      961 ggcacgttgt ctgagcgcga gcatccgttg gctcgttcgt cgcaggcgtt ggggcaggga
     1021 tgagcatgcg gtggcgcagc gtgcacggcg tctcttcggc gcaccgatgc cgtacaggtg
     1081 gctgcgcacg ctcggtctga aagtgcggca ggtcaacggg ggcggcggca atggcggcgg
     1141 ggtgcgcggc gagtggatcg agccagacgc ggagggcggc gacggcggag tgatcttcta
     1201 cattcacggc ggcggctacg tctcctgctc cgccgcgacg caccgcccca tcaccagttc
     1261 gctcgcgcgt ctgaccggca ggcgcgtctt cagcctcaac tatcgtctcg cgccggaaca
     1321 ccgttatccg gctgcgctcg acgatgcctt cgccgcttac gaatggttgc tcacgcacga
     1381 gaagatcgcg ccgggcgcga tctcttttgc gggcgattca gccgggggcg gcctcgtgct
     1441 cgcgtgcctg ctgcgtgcgc gcgacgcggg gctgccgctt cccgcctgcg ccgtctgttt
     1501 ctctccgtgg actgatctgg cgggggcggg cgagtcgcaa catttcaacg acggcctctg
     1561 cgcgatgttc tacacggaga acaacgcaga gtttgccgcc gcctatctgg gagacgcctc
     1621 gccgcgcgac ccttatgcct cgcccgctta cgcagagttt gccggactcc ccccgctcct
     1681 cctgcaagtg ggttcgaccg agttgcttct ggacgatgcg cggcgcgtgc accgcaaaat
     1741 tcaggcggcg aagggcacga gccgtctgga aatttacgaa gacgtagctc actgctggca
     1801 gatgctcgac ggcttcgtcc ccgaagcgcg cgccgcgctg cgacaggcgg cctcgttcat
     1861 ccttgccccg ctctcttctg caaacggcga cgccccaaag tcgtaacggc gacgcgcgcc
     1921 cgcgcgcctc cgcacttgca tcaacatcat acaaacacgt ctgacattgt ctcacctcat
     1981 aaattccgta gcgtctcgga gagcctgcgc gcggcgcggc tttgaacgtc ctatgtattt
     2041 gcatttcagc aactaggcgg ctttccctcc cggcgcgcac gggaaagttg cggctggcat
     2101 catgtttgct tttattcgag ggcaaatgag acacaaaatg agaagttcga agttttcagc
     2161 gtcggtggcg gccttggtct tctgcgtgct gtgcgcgccg cttccgctcg tcgcccaaca
     2221 gactgccgga cagcaacccg atccgctcga agccctttcg gtcaaggaga gggagatcgt
     2281 ggtcgctttt caggagcgcg tgaaggacta cgccaagctg cgcgagtcga tagaagccaa
     2341 gatgccgaaa ctcgccaagg agagcacgcc cgaagagatc gacgcgcaca agaagagttt
     2401 cgaagagcgc gtcagggctg cgaggacgga cgccaagccc ggccagatgt tcacgcccga
     2461 cattgcgaat tttatccgcg cgacgatccg aagcgagttc aaggggacgg agcgcaagga
     2521 actgcgcgcg tcggtcttgg aggcggacac gaagggcgtg cccctgcggg ttaattacac
     2581 ttacccggaa acgaaagagt tggttgagat gccgcccacg cttttgctga aactcccgaa
     2641 tctgccgaag cagttgcgct atcgttttgt tggccggaac atgctgctgg ttgaccggga
     2701 aaacggtctg atcgtcgatt acatgctcaa cgcgctgcct taaacttttc gcgcgggaga
     2761 agggaaacgc tatgaaacgt aatcttgctt cgctattaac actcgccgca ctcttctttg
     2821 gcggcaactt catcaaacct gcgctcggtc aaaatccttc cggtcaggcg accggcgtga
     2881 gggtcgcggc ctctacggcg gcggcggcga cgacggccac gccgcccgtc aggctaacac
     2941 tgccggtcaa agacggctcg gtgcgtttca tggtggtcgg cgacacgggg acgggcacac
     3001 agcagcaaca ggagttggcc gacgtgatgc tgcgctaccg ttcggtcttt ccgttcgagt
     3061 ttgccctgtt gatgggcgac aacatgtacg gcggggagaa ggccgaagac ttcaagaaga
     3121 agtttgagga catttacaaa ccgctgttgg acggcaaggt caagttctac gcctcgctcg
     3181 gcaatcacga cgagtcgaac cagcgtttct acgacctctt caacatgaac ggcgaggagt
     3241 attaccgctt cacgaaaggc aacgtcgctt tc
//
LOCUS       DASWNS010000662         4033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_388600, whole genome shotgun sequence.
ACCESSION   DASWNS010000662 DASWNS010000000
VERSION     DASWNS010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388600"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VGB76_22905"
     CDS             complement(<1..635)
                     /locus_tag="VGB76_22905"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0101794.1"
                     /translation="MADDDARRLQDLTIGEAKRIVVYGVALVLAVGLFLLLIGQVLVA
                     LLLGLVAGAFLLPVQEWLERRLRARSGSALITIALIVVPLVALAGYTWYELSGYSTNI
                     SQQERTKISDAISGALERYIPVSRAGTRATLEAAFTEGLTRSAAAIQDLRSRAALILA
                     SLAVFLFTVFYVLTHRTRISNYIKVRVPGDYLEFYEKLSDNVGGALRGALQA"
     gene            complement(738..2777)
                     /locus_tag="VGB76_22910"
     CDS             complement(738..2777)
                     /locus_tag="VGB76_22910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEY0101795.1"
                     /translation="MKTAATTTSAGCGLRSGAVLPRLLLCGLAMLLLSAAAAVPAQAQ
                     VIRSFTRRNPVTNERGNIALVGNSLMSCRARNNNNCAVTRSGSVTGSNSDYTSEFIDA
                     DSDGATENSSSATLTLPGGSTVLWAGLYWGARSTDPARDQIKFKPANSGFYSTLIASQ
                     LDSTTATSNAYQGFADVTAQVQSARSGTYWAADIAANSGQDTTGFYAGWSLVVVYRDV
                     NQPLRNLTVFDGFASVSSGQNVTTTASGLLTPTTGAFNAYAGTVTYEGDQGIFGDSFQ
                     MTNTSTNVTTTIADVPNPANNFFNSSISNLGTRITAKNPDYANQLGFDVDTVSIASAN
                     TVLGNGATSVRLTFNSTQDLYYPGVLTFAVDVFRPQVEGNITKTVDDPNGGSVNPGDV
                     LEYTIVVSNTGNDGAANNVLRDTIPSNTTYVAGSMQITAGANLGVKTDGAGDDQAEFD
                     GTRVVARLGTGANSANGGLLNPAESTTLKFRVKVNAATTDGTVVANQAVVNYHAATLG
                     DAFTSYSDGNPNVAGTQTTDVTVIVPPVPSVKLVKDCTSPANCLSAKQQPGTDLTYTI
                     TFTNDGNVTVQNLTIVDIIPITDTGSAIVRSTDFKVGSMTFNPGTSGLTIAPADYKYY
                     NDPLSTYPLLPPWTPISSYTPVGTYDPNVTYVGWKLTGSLPPGTSGSVTFTVRIK"
     gene            complement(2774..>4033)
                     /locus_tag="VGB76_22915"
     CDS             complement(2774..>4033)
                     /locus_tag="VGB76_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101796.1"
                     /translation="RLTDSLNGQEIGSNLVTVGAQYRPTDKLELSVKREQNLGEADPT
                     FPSQTTIAARYKWNQWANIFFTQRLASAPIIPISDTSSTGFASTGSRRETAIGIETKL
                     GRYTNVVSRYQLENGINGTDSFAVIGLQNRLPVTKRLSLDLGLEHGFHLAGEGQSFTG
                     GSFGFSYLPSKDMRTAARYELRNQTGSLAQLLTVGAAGRLGDGLTTLARFQWSRANFQ
                     GRENATMQGTAALALRPLKSDRAALLLSYTHRSLTQGGLNGQSDTTERSDTLSTDGLW
                     QPFKDTELYGRVALKFGANSRQGLMSASALTYLAQLRAQKRLRRAFDVAGEVRLTAQP
                     STHTTRTSAGAELGYWVFADMRVGIGYNFTSASEPGGIVLSQPRGFYFTISTKLSNLF
                     DLFGTAADGLLAPDTTAKPQDGQGAPR"
BASE COUNT          690 a         1132 c         1427 g          784 t
ORIGIN      
        1 gcctgcaacg cgccgcgcag tgcgccgccg acgttatccg agagcttctc gtaaaactcc
       61 agatagtcgc cggggacgcg caccttgatg tagttggaga tgcgcgtgcg atgcgtgagc
      121 acgtagaaga ccgtgaagag aaagacggcg agcgaggcga ggatgagcgc ggcgcggctg
      181 cgcaaatcct gtatggccgc cgccgagcgc gtgagtcctt cggtgaaggc ggcttcgagc
      241 gtggcgcgcg tgcccgcgcg actgacgggg atgtagcgtt cgagcgcgcc gctgatggcg
      301 tcgctgatct tcgtgcgctc ctgttgactg atgttcgtcg agtagccgga caactcgtac
      361 caggtgtagc ctgcgagcgc gacgagcggc acgacgatca gcgcaatcgt gatgagcgcg
      421 ctccccgaac gcgcgcgcag gcgacgctcc agccactcct gcacgggcaa cagaaacgcg
      481 cccgccacca gtccgagcaa cagagcgacg agcacctgcc cgatgagcag caggaacagt
      541 cccaccgcca gcacgagcgc aaccccgtac acgacgatgc gctttgcctc gccaatcgtc
      601 aaatcctgca aacgtcttgc atcgtcatcc gccatcgtgc gggtaatcta acacagggcg
      661 gggaaagaag tgatgagtga tgagtgatgg gtgatgagtt aaaacagaac ttgtctttca
      721 ctcatcactc atcactttta cttgatgcgg acggtgaaag tgacgctgcc ggaggtgccg
      781 ggcgggaggc tgcccgtcag tttccatccc acgtatgtga cgttcggatc gtaagtgccg
      841 acgggtgtgt agctcgatat gggagtccag ggcgggagca gaggataggt gctgagcgga
      901 tcgttgtaat acttgtaatc cgcaggggcg atggtcaggc cgctcgtgcc ggggttgaaa
      961 gtcattgagc cgactttgaa gtcggtgctt ctgacgatag cggagccggt gtcggtgatg
     1021 gggatgatgt caacgatggt caggttttgc accgtgacgt tgccgtcgtt cgtgaaggtg
     1081 atggtgtagg tcaggtcggt gccgggctgc tgcttcgcgc tcaggcagtt ggcgggcgag
     1141 gtgcagtcct tcaccagttt cacgctcggc acgggcggga cgatgacggt cacgtcggtc
     1201 gtctgcgtcc cggcgacgtt cgggttgccg tcgctgtaag aggtgaaggc gtcgccgagc
     1261 gtagccgcgt gatagttgac gaccgcctga ttggcgacga ccgttccatc cgtcgtggcg
     1321 gcgttgacct tcactctgaa tttaagcgtc gtagactccg ccggattgag caacccgccg
     1381 ttcgctgaat tcgcgcccgt tcccagacga gcgacgacgc gcgttccgtc gaactccgcc
     1441 tgatcatcgc ccgccccgtc ggtcttcacg ccgaggttgg ctcccgctgt gatctgcatg
     1501 ctcccggcca cgtaggttgt attcgacggg atcgtatcgc gcaagacatt gttggcggct
     1561 ccgtcgttgc ccgtgttgga gacgacgatg gtgtattcga gcacgtcgcc ggggttcacg
     1621 ctcccgccgt tcgggtcgtc tacggtcttc gtgatgttgc cctcgacctg aggccggaag
     1681 acatctacgg cgaacgtgag cacgcccgga taatacaagt cctgtgtcga gttgaaggtg
     1741 aggcgcacgc tggtcgcgcc gttgccgagc acggtgttcg cgctggcgat gctcaccgtg
     1801 tcaacgtcga agccgagttg gttggcgtag tcgggatttt tggcggtgat gcgcgtgccg
     1861 agattgctga tgctgctgtt gaaaaaattg ttggccgggt tcggcacgtc ggcgatggtg
     1921 gtcgtcacgt tggttgacgt gttggtcatc tgaaagctgt cgccgaagat accctgatcg
     1981 ccttcgtagg tgactgtgcc tgcgtaggcg ttgaaggcgc cggtggtggg cgtcaacaaa
     2041 ccgctcgcgg tggtggtgac gttttggccg ctgctgacgg aggcgaaccc gtcgaagacc
     2101 gtgaggttgc gcaaaggctg gtttacgtct cgatagacga cgacgagcga ccacccggcg
     2161 taaaaaccgg tggtgtcctg cccgctgttg gccgcgatgt cggcagccca gtaggttccg
     2221 ctgcgggcgg actgaacctg cgcggtgaca tcggcgaagc cttgataggc gttcgaggtg
     2281 gcggtggtgc tgtcgagttg ggaggcgatg agggtggagt agaaaccgga gttggcgggc
     2341 ttgaatttga tctggtcgcg cgcggggtcg gtcgaacgcg cgccccagta cagccccgcc
     2401 cagagcacgg tggagccgcc cggcagcgtc agcgtcgcgc tgctcgaatt ctccgtcgcg
     2461 ccatcgctgt cggcatcgat aaactcggag gtgtagtcgc tgttgctgcc ggtcacactt
     2521 ccgctgcgcg tgacggcgca gttgttgtta ttccgcgcac gacaagacat gagcgagttg
     2581 ccgacgaggg cgatgttacc gcgttcgttc gtcaccgggt tgcggcgggt gaacgagcgg
     2641 atcacctgcg cctgcgcggg cactgccgcc gccgcagaga gcaggagcat ggcgaggccg
     2701 cagagcagga ggcgtggcaa gaccgcgccg gagcgcagcc cgcagcccgc acttgttgtc
     2761 gtcgccgcag ttttcaacga ggtgctcctt gtccgtcttg cggcttcgcg gtggtgtccg
     2821 gcgcgagcag cccatcggcg gcggtgccga agaggtcgaa gaggttggag agcttggtgg
     2881 agatggtgaa gtagaagccg cgcggctgcg agaggacgat gccgccgggt tcgctggccg
     2941 aagtgaagtt gtaaccgatg ccgacgcgca tgtctgcgaa gacccagtag ccgagttccg
     3001 ccccggcgga ggtgcgcgtc gtgtgcgtgg agggctgcgc cgtgaggcga acttcgcccg
     3061 cgacatcgaa ggcgcggcgc agacgctttt gcgcgcggag ttgtgccagg taggtcagag
     3121 ccgacgcgct catcaagcct tgacggctgt tcgcgccgaa cttgagagcg acgcgcccgt
     3181 aaagttccgt atccttgaaa ggctgccaga gtccgtcggt cgaaagcgtg tcggagcgtt
     3241 cggtcgtgtc ggactgcccg ttgagtccgc cctgcgtgag cgagcgatgc gtgtaactga
     3301 ggaggagcgc ggcgcggtcg gacttgagcg ggcgcagggc gagcgcagcc gtgccctgca
     3361 tcgtggcgtt ctcgcgcccc tgaaagttgg cgcgcgacca ttggaagcgg gcaagcgtgg
     3421 tcagcccatc gccgaggcgg ccagccgcgc cgacggtgag cagttgcgcg aggctgcccg
     3481 tctggttgcg caactcgtag cgcgccgccg tcctcatgtc cttcgaaggc aggtaggaga
     3541 agccgaacga gccgccggtg aaactctgcc cttcgcccgc gagatgaaag ccgtgctcca
     3601 atccgagatc gagcgacaac cgcttcgtga cgggcaggcg attctgcaaa ccgatgacag
     3661 cgaaactgtc cgtgccgttg atgccgttct caagttgata gcgactgacg acgttcgtgt
     3721 aacgaccgag tttcgtctcg atgccgatgg cggtttcgcg gcgcgagccg gtggaggcga
     3781 agccggttga tgaagtgtcg ctgatgggaa tgatgggcgc ggaggcgagc cgctgcgtga
     3841 agaagatatt cgcccactgg ttccacttgt agcgcgcggc gatggtggtc tggctgggga
     3901 aggtcgggtc ggcctcgccc agattctgct cgcgcttgac ggagagttcg agcttgtctg
     3961 tcgggcgata ctgcgcgccg acggtgacga ggttcgaacc gatctcctgc ccgttcagcg
     4021 aatcggtgag gcg
//
LOCUS       DASWNS010000663        15152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_25217, whole genome shotgun sequence.
ACCESSION   DASWNS010000663 DASWNS010000000
VERSION     DASWNS010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15152
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25217"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..776)
                     /locus_tag="VGB76_22920"
     CDS             complement(69..776)
                     /locus_tag="VGB76_22920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0101797.1"
                     /translation="MKKLMLEELRSLQTMETAPTTAHAVIKVAIVEDRREIREGLAML
                     INGTDGYRCTGSYGSMEEAFAKLGGNLPDVLLSDIGLPGMDGIEGIRILKERHPALLV
                     LMLSVYDDNERIFDALCAGACGYLLKKTPPARLIESLKEAAAGGAPMSPEIARKVVTL
                     FRDFRPPERADYQLTPHEMRLLKLLVEGHSYKTAATELGVTVNTVSFHLRHIYEKLQV
                     HSKSEAVAKALRDHLIK"
     gene            complement(850..4047)
                     /locus_tag="VGB76_22925"
     CDS             complement(850..4047)
                     /locus_tag="VGB76_22925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY0101798.1"
                     /translation="MSLGCLLLLLAAATRAEQLPVRVYTSADGLGQNRVKRIVRDSRG
                     FLWFCTADGLSRFDGYRFTTYDTSQGLPYPSINDLLETRAGVYWIASNGGGVFRFNLS
                     GGASGGSGAAPVNPDAAQTRSRFTVYKLGSTPRMNRPNRLFEDAAGRIWAGTDDGLFV
                     FDGSNGTDAFQRVELDDGRETNTPIPVWAFTEDDERKLWIATSTGLWRREPDGRISAF
                     PIRPFIKGAVWDVSKDASGRLWVGHSDGLLIFKLPAARAGVPVDESSNLRVRVPQTSS
                     PQLLLPDEAGEAYAYTTADGLAHEVVRQIHHAADGRIWAGTARGLSLFGAQSFSNYTS
                     AHGLSDENIRALAEDRDGNLWIGTETGGAIKLARHGLVTYREADGLNGTQVRDIFETR
                     TGVLCVITDNGFINYFDGQRFAAVRPNMPPEIKQLMGVVEDARGDWWIGTGLGLYRFS
                     GLRHVSELARARPSHYTSRNGLSSDAVTRVFEDSRGDIWLGMDSDHVLARWERAADKF
                     HTYREGLPGRSQVLSFAEDRSGAVWAGFREGGAARYRAGRFELFNESSGLTADEILKM
                     YVDEAGRLWFGSNSYGLVRIDDPAAERPAFRIYSTAEGLTSGIIRSIAEDNFRRLYLG
                     TSRSVDRFDPATGRIKRYTIADGLAANESQVAYRDRQGALWFGTWNGISRLIPEPERP
                     ASPPPVMIDNLQIAGVRHPVSDLGETQIGNLRLSHTQNQVQVDFLGLGFGAGELLRYQ
                     YKLEGAAGGVDWSAPADARSVTYANLSPGSYRFLVRAVNTDGIVSAVPASVEFQILRP
                     VWLRPWFLLGGALLVGGFVFAVARARLKRLVELERVRTRIATDLHDDIGSSLSQIAIL
                     SEVVSQRVPQDNAPVIEPLSLIAGTSREMVDSMSDIVWAINPERDHLSDLTQRMRRFA
                     SDILSARDIDFRFRAPAAEKDIRLGADLRREVYLIFKECVNNLVKHSGCTEAEVELRI
                     VNHWIVLRISDDGRGFDPSTSPSGGNGGNGASLGGHGLTSMRRRAVALGGTFEVKSAQ
                     GRGTVSTLKVPARGGGRMQQSGYRLRDGWRKLLHK"
     gene            complement(4187..5518)
                     /locus_tag="VGB76_22930"
     CDS             complement(4187..5518)
                     /locus_tag="VGB76_22930"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0101799.1"
                     /translation="MSERIYRDPVHNIIRLRTDTREGLLLVNLIDAPEFQRLRRIKQL
                     GLALYTYQGAEHSRFAHSLGVLHLITRVLDRLGENYRIAEEDRAAARAAALLHDIGHG
                     SFSHVMENVLGFHHEAWTVRAVLAEETEINAVLREYSPELPRRVADIIEGKFQPAFLG
                     QLVSSQLDVDRMDYLLRDSLMTGAKYGIYDLEWIINALAVDEENDRIYVAARGIYAVE
                     DYLQARYYMFRQVYFHRTLRSAEAVLRSVLRRALELVAAGEEVWCAPGTAFEKVLHRQ
                     PLTLEDHLEMDDSDVLFHLKQWRRAKDTVLNDLSNRFVGRRLFKAIDLDMPAEERAQF
                     LSLARAAVTRAGFEPRFYFIEDHAGDVPYYNYYTAEGAEPKARIYVEDGYARPAIREI
                     SEVSGAVRGLRRYQLDRVCFPPEVKEEIYALYHTASPVAATAATRAVEAGG"
     gene            complement(5591..6598)
                     /locus_tag="VGB76_22935"
     CDS             complement(5591..6598)
                     /locus_tag="VGB76_22935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101800.1"
                     /translation="MSSTSRSVASVVLFAASLLCAAADARAQLGGVHTLYGDLKVDES
                     KTTGLKPLAFDILLYTEGGVLVTRQSVPGGGRYRFNNLRDGRYELVVEVEHMEVARIR
                     QISVISPFKNDFRQDILLEWREDAARRPAPRAATVSVDDLYQRNAANQKLFTRAGEAL
                     DKQEYERGIALLRQVVEADPHDFQAWTELGTAYFVRNNMTEAEKSYARALEARPTFSL
                     ALVNLGRLRLAQKNYDGAIELLSKAVKIRPQSPGANYYLGEAYLQVKKGSVAVVHLNE
                     ALRLDPEGRAEAHLRLALFYHAAGLRDKAAAEYAQFLTKRPNHPDRKKLERYIAENKQ
                     P"
     gene            complement(6816..10652)
                     /locus_tag="VGB76_22940"
     CDS             complement(6816..10652)
                     /locus_tag="VGB76_22940"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0101801.1"
                     /translation="MKDQLSARSRAALALCGFLLSLMVCGSALAQTGTSSVRGTVADP
                     QGNVIPGASVTLLNTDTNFSRTTTTSDDGVFSFELIPPGTYRVEAEAANFKKSVVTDV
                     RALVAKPTELSLALEVGSVSETVTVASTANEALLNTQDATLGNNFVSQQITQLPLEAR
                     NVAALLTLQPGVTRTGYVAGARSDQSNVTLDGVDINEAQNNQIGTVDSSSDNDPSTGT
                     VLRLNAEAIEEFRVTTVNANAQAGRSSGAQISLITKGGSNDIKGAGFWFHRPTILTAN
                     DFFNNRSGVERPALIRNTFGGAIGGPIIKDRAFFFYSFEGRRDRSQTSVLSRVPLPSM
                     GRGELRYENASGGVTTLTAENIRTIFPELNGVNPLATAVLADAARRYPANDFGSTGDS
                     FRDAGGLVLLNTAGYRFNASQPVDLNSHVARFDLNLTKNQTIFARTNVIYDLIAGTPV
                     FPDTPRPDTWSHPWGTAIGHTWTPSSRFVNRFTYGLTREAFTQGGDAAKNEVYFRFVF
                     FPVLDSRTINRTTPTHNITDDFSWVAGNHTAQFGTNIRIIRNRRTTFANAFDVALTNP
                     SYYEDSGTPLFTSVDDFSPAGNGQTAVIQNAVSALLGRLTQYTARFTFDLEGNPLAPG
                     TPTKREFATEEYDFYAQDSWKMRPNLTLTYGLRYGLSRPIYETQGYETKPNIALSEYF
                     RRRQAASLRGQNYDEPLLVELSGPANGKEPMYPWDKNNFQPRIGVAWSPNFKNKFLGG
                     LFGKESQSVIRGGFAMTNDYIGQALAVRFDLNNALGFSSETTSPPGLCNTTDRPCPLF
                     TSFSQEVRGLPGITVPGRLTFPQSQPQDLSLRIESSLDEEIKSPTNYQWSLTFERQLP
                     KGMIVQAAYVGRLGRNLLASRDVMMPNNLTDPQSGMDWYTAAGILEDIRRNFANQGIT
                     SSSPRAAQQAAIRSIAPIPYFENLFGGVPLNTFARTLLGNSRAPFATNYTQAVLGDAL
                     IFNGNDWSLTQVDIDNALLAGGRNHLFYQSQYGALSSFSTIANSDYHAGTLSIRQRLG
                     ESLTADFNYTLSKSMDDASGLQNGGSFGAALILNPLRQRDNYSVSDFDVRHIINANAV
                     WQLPIGRGRWINTENKFVNAVVGGWQMSGIYRWNSGLPLSAPFDDARWATNWQVQSNG
                     VRIRPIEACPTRGGTDAPAVFCNRNAAYQSFRNPKPGETGDRNVFRLPGYVVLDMGMS
                     KSFTMPWSETQKLQLRFEAFNVTNTQRMGGLAGGRDGYGLVLDPQNDTPPSNWSNFSS
                     IQGDRRVMQFGFRFEF"
     gene            complement(11222..11887)
                     /gene="ribA"
                     /locus_tag="VGB76_22945"
     CDS             complement(11222..11887)
                     /gene="ribA"
                     /locus_tag="VGB76_22945"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEY0101802.1"
                     /translation="MEETPQQTSTTCQWATTNLSVERVSVANLPTAFGEFKIAGYRAP
                     ESGEEFVALFKGEIERETPTLARIHSQCLTGDVFGSLKCDCGAQLHQAMRMIEQEGRG
                     AIVYQLQEGRGIGIINKIRAYALQDNGADTVEANERLGLAVDLREYRQCAEVLFDLGL
                     CRVRLLSNNPLKLRAMEEAGLQVVERVSIQVPHTEAAAHYMRTKKEKLGHLLENIERE
                     SIK"
     gene            12124..13110
                     /locus_tag="VGB76_22950"
     CDS             12124..13110
                     /locus_tag="VGB76_22950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0101803.1"
                     /translation="MEEQNFGEITLPADPPTSHVRRPALVFMRGEQLAAPIPLERDEV
                     ILGRALEADVRVNDARASRLHARISTELDAETNTSRYRLTDLGSTNGTLLNGQPVTEA
                     FLQNGDKLIIGEHLIRFDLLDDLDREFQRQLYRLIAHDELTGLLTSKSFFSELRREAA
                     RAESEGRPFCVLMMDLDHFKQVNDTCGHLVGSQTLEEIGGLITRALRAGDVAARFGGE
                     EFAAFLLDADCAQALVAAERVRTAIEEHAFSSTRHGTNENRTLHITISIGVAAYPDDA
                     RDPIELIELADTALYHAKQSGRNRVSAFCLAPSDDETPRELRPRNAVLPRDE"
     gene            13400..14122
                     /locus_tag="VGB76_22955"
     CDS             13400..14122
                     /locus_tag="VGB76_22955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101804.1"
                     /translation="MLKFTFVPRVACGLAVALFIYGLAATAAPAQAQATQPAQRRAVA
                     PVTTSQAPAQIPAPSPTPAATATVATDNHDLSINANVTARELHFEVVPNPTVEFPGQP
                     RRDTVWEAERTNLPAQVQPGVTYRDIGIRLKITSVFADIERIVAEALGELPARDGDNN
                     PPTPEPNPNDAPPQIENAPQATPGATQTNAAPQAASAPIQAAASTQTPRAPFAQTTSA
                     PQTAPTRQKSSSHATVRRGRHQ"
     gene            14119..14556
                     /locus_tag="VGB76_22960"
     CDS             14119..14556
                     /locus_tag="VGB76_22960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101805.1"
                     /translation="MNRVVRRIALAALFCAALCIAVAAQQTPPRDATVTNPPTRRTTP
                     PLSPGAQNAGAANVEENFELNIAERRITEQDFFASTAIETEAGEAHGLSLRVGVAVGA
                     SEIDVLLRNVRGSVRFRGSLDALRRVLDARRETNAAPPPAGVP"
     gene            14668..>15152
                     /locus_tag="VGB76_22965"
     CDS             14668..>15152
                     /locus_tag="VGB76_22965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101806.1"
                     /translation="MNKLSSLGAIIVAICVACLAPVATQAQTPSPSASTTTARGTDNA
                     SIFANSTSQQAQPRPTPSPIAPVRPLSQEDIQRQQAASERRRQSAASGDPDVLLDVPN
                     LSVEEITLEVENLRAKVSLDARLANLLQLSAGADAGIDKVKLTIKGVRAELLLKVRLD
                     NV"
BASE COUNT         2815 a         4621 c         4905 g         2811 t
ORIGIN      
        1 gggcggaacc gcgccgcaga ggaaagaaag aaagaaagaa agagcagcaa gagcagcgcg
       61 ggcgagagct acttgattaa atggtcgcgc agggctttgg cgacggcttc tgacttggag
      121 tgaacctgta gcttttcgta aatgtggcgc aggtgaaacg agacggtgtt gaccgtgacg
      181 ccgagttcgg tcgcagccgt tttgtagctg tggccttcga cgaggagttt caacagacgc
      241 atctcgtgcg gcgtcagttg atagtcggcg cgttcgggcg ggcgaaaatc gcggaagagc
      301 gtaacgacct tgcgcgcgat ctccggcgac atcggcgcgc cgcccgccgc agcctccttt
      361 aaactctcga tcaggcgcgc gggcggcgtc ttcttgagga gatagccgca cgcccctgcg
      421 cacagcgcgt cgaagatgcg ttcgttgtcg tcgtaaaccg agagcatcag gacgaggagc
      481 gcgggatggc gctctttaag gatgcggatg ccctcgatgc cgtccatgcc gggcaacccg
      541 atgtctgaga gcaacacgtc gggcaggttg ccgccgagct tcgcgaaggc ttcttccatc
      601 gagccgtagc tgcccgtaca gcgataaccg tccgtgccgt tgatgagcat tgccagccct
      661 tcgcgaatct cgcgccggtc ttcgacaatc gccaccttga tcacggcgtg agccgtcgtc
      721 ggcgcagtct ccatcgtctg taaactcctc aactcttcga gcatcaattt cttcaacttc
      781 ctctcctgcg ggtttcgacg gcacggcggg aagtagagag tacgcccgca ggcacgcagg
      841 cgtcactacc tatttatgta gtaactttcg ccagccgtcg cgcaggcggt agccgctctg
      901 ctgcatgcga ccgccgccgc gcgccggaac tttcaacgtc gagacggttc cgcgcccctg
      961 cgcggacttc acttcaaacg tgccgccgag cgcgacggcg cggcgacgca tgctcgtcaa
     1021 gccgtgcccg ccgagactcg caccattgcc gccgttgccg ccggacggcg aagttgacgg
     1081 gtcgaagccg cgcccgtcgt cgctgatgcg cagcacgatc cagtggttga cgatgcgcag
     1141 ttccacttcc gcctccgtgc agccggaatg tttgacgaga ttgttgacgc actctttgaa
     1201 gatgagatag acctcgcggc ggaggtctgc gccgaggcgg atgtcctttt cggcggcggg
     1261 cgcgcggaag cggaagtcga tgtcgcgtgc gctcagaatg tcgctggcga agcggcgcat
     1321 gcgttgggtg agatcgctca ggtggtcgcg ctcagggttg atcgcccaca cgatgtcgct
     1381 catcgaatca accatctcac gcgacgttcc ggcgatgagc gagagcggtt cgataacggg
     1441 cgcgttatcc tgcggcacgc gctggctgac gacttcgctc aggatcgcga tctgcgacag
     1501 gctcgacccg atgtcgtcgt gcagatcggt ggcgatgcgc gtgcgcacgc gttccaactc
     1561 gacgagtcgt ttgaggcgcg cacgcgcgac ggcgaagacg aatccgccga caagaagcgc
     1621 gccgccgagc aggaaccagg ggcgcagcca gacggggcgc aggatttgaa actcgacgct
     1681 cgccgggact gcgctcacga tgccgtccgt gttgacggcg cgcacgagga agcgataaga
     1741 gccgggcgag aggtttgcgt aggtgacgct gcgcgcatcc gcaggcgcgc tccagtccac
     1801 gccgcccgcc gcgccctcta acttatattg atagcgcaac agttcgcccg ctccgaagcc
     1861 gaggccgaga aagtctacct gcacctgatt ctgcgtgtgg ctcaggcgca ggttgccgat
     1921 ctgcgtctcg ccgaggtcgg agacgggatg gcgcacgccc gcgatctgca aattatcgat
     1981 catcacgggc ggcggcgacg cggggcgttc cggttcgggg atgagccgcg agatgccgtt
     2041 ccaggtgccg aaccagagcg cgccctgacg gtcgcgataa gcgacctgcg actcgttggc
     2101 ggccagcccg tcggcgatgg tgtaacgctt gatgcgtccc gtcgccggat caaaacggtc
     2161 tacgctgcgc gaggtgccga gatagaggcg tcggaagttg tcttcggcga tactgcggat
     2221 gatgccgctc gtcagccctt cggcggtcga atagatgcgg aaggccggac gctccgccgc
     2281 cgggtcgtca atccgcacga ggccgtaaga gttgctgccg aaccagagac gccccgcctc
     2341 gtccacatac attttgagaa tctcgtcggc ggttaaccct gaggattcgt tgaacaactc
     2401 gaagcgtccc gcacggtaac gcgccgcgcc gccctcgcgg aaacccgccc acaccgcgcc
     2461 cgacctgtct tccgcgaacg acaacacctg gctgcgaccc ggcagccctt ccctgtaagt
     2521 gtgaaacttg tccgccgcgc gttcccaacg cgcgagcaca tggtcgctgt ccatgccgag
     2581 ccagatgtcg ccgcgcgagt cttcaaagac gcgtgtgacc gcatcgctgc tcaggccgtt
     2641 ccttgacgtg tagtgcgaag ggcgggcgcg tgccagttcg gagacgtggc gcaggccgga
     2701 gaagcgatag aggccgaggc cggtgccgat ccaccaatcg ccgcgcgcgt cctcgacgac
     2761 gcccatgagt tgtttgatct ccggcggcat gttcgggcgg acggcggcaa acctctgccc
     2821 gtcgaaatag ttgatgaagc cgttgtctgt gatgacgcag agcacgcctg tacgagtctc
     2881 gaagatgtcg cgcacctgtg tgccgttgag gccgtccgct tcgcggtagg tgacgaggcc
     2941 gtgacgcgcg agcttgatcg cgccgcccgt ctccgtgccg atccagagat tgccgtcgcg
     3001 gtcttcggcg agcgcgcgga tgttctcgtc gctcaagccg tgcgcactcg tgtagttgga
     3061 gaaactctgc gcgccgaaca ggctgaggcc gcgcgccgtg cccgcccaga tgcgaccgtc
     3121 ggcggcgtgg tgtatctgtc gcaccacttc atgcgcgagt ccgtcggcgg tcgtgtaagc
     3181 gtaggcctcg cccgcctcgt caggcagcag gagttgcggc gaagaggtct gcggcactct
     3241 cacacgtaaa tttgaagact catccaccgg cacgcccgca cgcgcggcgg gcagcttgaa
     3301 gatgagcagg ccgtcgctat gtccgaccca gagacgcccg gatgcatctt tgctcacgtc
     3361 ccagaccgcg cctttgataa aggggcgaat gggaaaagcc gagatgcgcc cgtccggctc
     3421 gcgtcgccac aaacccgtag aggtggcgat ccagagtttg cgctcgtcgt cttcggtaaa
     3481 tgcccagacg gggatgggcg tgttcgtctc gcgcccatcg tctaactcga cgcgttgaaa
     3541 cgcgtccgtg ccattcgacc cgtcaaagac gaacagcccg tcgtccgtgc ccgcccagat
     3601 gcgccccgcc gcatcttcaa agagccgatt cgggcgattc atgcgcggcg tgctgccgag
     3661 tttatagacg gtgaagcgcg aacgcgtctg agccgcatcc gggttgacgg gcgcagctcc
     3721 gcttccgccg cttgcgccgc ccgataagtt gaagcgaaag acgccgccgc cgttgctggc
     3781 gatccagtag acgcccgcgc gcgtttcgag cagatcgttg atggaaggat agggaagtcc
     3841 ctgcgaggtg tcgtaggttg tgaagcggta gccgtcgaag cgcgagaggc cgtcggcggt
     3901 gcagaaccag aggaagccgc gcgagtcgcg cacgatgcgc ttgacgcggt tctgccccaa
     3961 cccgtcggcg gacgtgtaga cgcgcacggg caactgctcc gctcgcgtcg ccgccgcgag
     4021 caacaacagc aggcagccga gcgacatgag cgcaaatccg cgacgcgaaa attgaagggc
     4081 agcgtttgac agggacacag ggaagcgacc tttacagcgt gaatgaagcg aagcgtcttt
     4141 acggtcgagg cgcgtcttgc agtcgccgaa ccgggcgcga ggtctttcag ccaccggctt
     4201 cgacggcgcg cgttgccgct gtcgccgcga cgggcgaggc cgtgtgatag agcgcgtaaa
     4261 tctcttcttt cacttcgggc gggaagcaga cgcggtcgag ttgataacgg cgcaggccgc
     4321 gcaccgcgcc gctgacttcg ctgatctcgc gtatcgcagg gcgcgcgtaa ccgtcttcga
     4381 cgtagatgcg cgctttgggt tccgcgcctt ccgccgtgta gtagttgtaa taaggcacgt
     4441 cgcccgcgtg gtcttcgatg aagtagaagc gcggctcgaa cccggcgcgg gtgacggcgg
     4501 cgcgtgcaag cgagagaaac tgcgcgcgct cctctgcggg catgtcgaga tcaatcgctt
     4561 tgaagagacg gcgaccgacg aagcgattgc tcagatcgtt taagaccgta tccttcgcgc
     4621 gccgccattg cttgagatga aagagcacgt cggagtcgtc catctccaga tggtcttcga
     4681 gcgtgagcgg ctggcggtgc aggactttct cgaaggcggt gccgggcgcg caccacacct
     4741 cttcgcccgc cgcgacgagt tcgagcgcac gtcgaaggac ggagcgcagg acagcttccg
     4801 ccgaacgcag cgtgcgatga aaatagacct gccggaacat gtaatagcgc gcctgtaaat
     4861 agtcctcgac ggcgtagatg ccgcgcgccg ccacgtagat gcggtcgttc tcttcgtcca
     4921 cggcgagcgc gttgatgatc cattcgagat catagatgcc gtacttcgcg cccgtcatca
     4981 gggaatcgcg caggaggtag tccatgcgat ccacgtccaa ctgcgaagag acgagttgac
     5041 cgagaaaggc gggctggaac ttgccttcga tgatgtcggc gacgcggcgc ggaagctccg
     5101 gcgaatattc gcggagcacg gcgttgatct ccgtctcttc ggcgagcacg gcgcgcaccg
     5161 tccatgcttc gtgatgaaag cccagcacgt tctccatgac gtgcgaaaac gagccgtgtc
     5221 cgatgtcgtg caggagtgcc gccgcacgcg ccgccgcgcg atcctcttcg gcgatgcggt
     5281 agttttcgcc cagacgatcc agtacgcgcg tgatgagatg cagcacgccg agagagtgtg
     5341 cgaagcgcga atgctccgcg ccctgatagg tgtagagcgc aagcccgagt tgcttgatgc
     5401 ggcgcaggcg ttgaaactcc ggcgcgtcga tcaggttcac gagcagtagt ccctcgcgcg
     5461 tatctgtgcg caggcgaatg atgttatgca cggggtcgcg gtagatgcgt tcgctcatcg
     5521 cactcatcgt aaagggtaaa tggtaaatgg taaatagagc gcgaaggccg gtgcgcggga
     5581 aacgtaagtt tcagggctgc ttattttccg cgatgtaacg ctcaagtttt ttccggtcgg
     5641 gatgattggg acgcttggtg agaaactgtg cgtactcggc ggcggctttg tcgcgcagcc
     5701 cggcggcatg atagaacagc gcgagacgca ggtgcgcctc cgccctgcct tccgggtcga
     5761 gccgcaacgc ttcgttgaga tggacgaccg cgaccgagcc ttttttgact tgcaggtatg
     5821 cttcgcccag atagtagttt gcgccgggag actgcgggcg tatcttgacg gctttcgaga
     5881 gcagttcgat tgcgccgtcg taatttttct gcgcgagacg tagcctgccg agattcacaa
     5941 gcgcgaggct gaaagtgggt cgcgcttcaa gagcgcgggc gtaggatttt tcggcctccg
     6001 tcatgttgtt gcggacgaag taggccgtgc cgagttcagt ccacgcctga aagtcgtgcg
     6061 ggtcggcttc gaccacttgc cgcaggagcg cgatgccgcg ttcgtactcc tgcttgtcca
     6121 gagcctcacc cgctctggtg aagagtttct ggttcgccgc gttgcgttgg tagagatcat
     6181 ctacggagac ggtggccgcg cgcggggctg gacgccgcgc cgcatcctcg cgccattcca
     6241 acaggatgtc ctgccggaag tcgttcttga agggagagat gacgctgatc tgccggatgc
     6301 gcgcgacttc catgtgctcg acctcgacga cgagctcgta tctcccgtcg cgcaggttgt
     6361 tgaaacggta gcgaccgccg cccggcacgc tctggcgtgt gacgagcacg ccgccttccg
     6421 tgtagagcag tatgtcgaag gcgagcggct tgagtccggt ggtcttgctc tcgtcaactt
     6481 tcagatcgcc gtagagcgtg tgcacgcctc cgagttgggc gcgggcatcg gcagcggcgc
     6541 acagcagcga cgctgcgaac aggacgacag aagcgacaga cctcgatgtg ctcgacatga
     6601 ccgttcctcc gaagcgccat tttaccaccg ccgcgagagc gaagggatga gggatgaggg
     6661 atgagggatg agggatgagg gatgagggat gaataagaag aagcattcaa ctcggttgag
     6721 agctttttct gcttcatccc tcatccctca tccctttaaa aaacggcggg tcgaaagccc
     6781 gtgccccgac ccgccgcaag tagttgtgag caagtttaaa actcgaagcg gaagccgaac
     6841 tgcatcacgc gacggtcgcc ctgaatggag gagaagttcg accagttcga gggcggcgtg
     6901 tcgttctgcg gatcgagcac gaggccgtaa ccgtcgcgcc cgcccgccag cccgcccatc
     6961 cgctgcgtgt tcgtgacgtt gaacgcttcg aagcgaagtt ggagtttctg cgtctcgctc
     7021 caaggcatcg tgaacgactt gctcatgccc atgtcgagca cgacgtagcc ggggagacgg
     7081 aagacgttgc ggtcgccggt ttcgccgggt ttcggattgc ggaaactctg gtaggcggca
     7141 ttacggttgc agaagacggc gggcgcatcg gttccgccgc gcgtcgggca ggcttcgatg
     7201 gggcgtatgc ggacgccgtt gctctgcacc tgccagttgg tcgcccagcg cgcatcgtcg
     7261 aagggagccg agagcggcag gccggagttc cagcgataga tgccggacat ctgccagccg
     7321 ccgacgaccg cgttgacgaa cttattctcc gtattgatcc agcgaccgcg accgatgggc
     7381 agttgccaca cggcgttggc gttgatgatg tggcgcacgt cgaagtcgga gacggagtag
     7441 ttgtcgcgct ggcgcagcgg gttgaggatc aaggccgcgc cgaagctgcc gccgttctgc
     7501 aagccggagg cgtcgtccat agatttcgac agagtgtagt tgaagtcggc ggtgaggctt
     7561 tcgccgagcc gctggcgaat ggagagcgtg cccgcgtgat agtccgagtt ggcgatggtg
     7621 gagaaggacg agagcgcacc gtactgcgat tgatagaaca ggtgattgcg ccctccggcc
     7681 agcaacgcgt tgtcgatgtc aacctgtgtg agcgaccagt cgttgccgtt gaagatgagc
     7741 gcatcgccga gaacggcctg cgtgtagttc gtggcgaaag gggcgcggct gttgccgagc
     7801 agggtgcgcg cgaaggtgtt gagcgggacg ccgccgaaga ggttctcgaa atacggaatg
     7861 ggcgcgatgc tcctgatcgc cgcctgctgc gcggcgcgcg gcgaagagga ggtgatgccc
     7921 tgattagcaa aattgcggcg gatgtcttcc agtatccccg ccgccgtgta ccagtccata
     7981 ccggattgcg ggtcggtgag gttgttgggc atcatcacgt cgcgcgaggc gagcaggttg
     8041 cgcccgaggc gtcccacgta ggcggcctgc acgatcatgc ccttcggcag ttgacgctcg
     8101 aaggtcaggc tccactggta gttggtcggc gacttgatct cctcgtcgag cgacgattcg
     8161 atgcgcagag acaagtcctg cggctggctc tgcgggaaag tcaggcgacc cggcacggtg
     8221 atgcccggca acccgcgcac ctcctgcgag aaggaggtga agagagggca cgggcgatcc
     8281 gtcgtgttgc agagtccggg cgggctggtc gtctccgagg agaagccgag cgcgttgttg
     8341 aggtcgaagc gcacggccaa cgcctgaccg atatagtcgt tggtcatggc gaagccgccg
     8401 cgaatcaccg actggctctc tttgccgaag aggccgccga gaaacttatt cttgaaattg
     8461 ggcgaccacg cgacgccgat gcgcggctgg aaattgttct tgtcccatgg atacataggc
     8521 tctttgccgt tagccggacc tgacagttcg accagcaacg gctcgtcgta gttctgcccg
     8581 cgcagcgacg cggcctgacg gcgacggaaa tactccgaga gcgcgatgtt cggtttcgtc
     8641 tcgtagcctt gcgtctcgta gatcgggcgg ctcaagccgt agcgcaggcc gtaggtcagg
     8701 gtcaggttcg ggcgcatctt ccaggagtcc tgcgcgtaga agtcgtactc ttcggtggca
     8761 aactcgcgct tcgtcggagt gccgggcgcg aggggattgc cttcgaggtc gaaggtgaaa
     8821 cgcgccgtgt actgcgtgag ccgcccgagg agcgcggaga cggcgttctg gataacggcg
     8881 gtttgaccgt taccggcagg cgagaagtcg tctacggaag tgaagagcgg cgtgccggag
     8941 tcttcgtaat aggagggatt ggtgagcgcc acgtcgaagg cgttggcgaa ggtggtgcgg
     9001 cggttgcgga tgatgcggat gttcgttccg aactgcgccg tgtgattacc tgcgacccag
     9061 gagaagtcgt ccgtgatgtt atgcgtgggc gtggtgcggt tgatcgtgcg cgagtcgagc
     9121 acggggaaga agacgaagcg gaaatagact tcgttcttcg cggcgtcgcc gccctgcgtg
     9181 aaggcttcgc gcgtcaggcc ataggtgaag cggttgacga agcgactgct cggcgtccac
     9241 gtgtggccga tggccgtgcc ccacgggtgc gaccaggtat cggggcgcgg cgtgtcggga
     9301 aatacgggcg tgcctgcgat caggtcgtag atgacattgg tgcgagcaaa gatggtctgg
     9361 tttttggtga ggttcaggtc gaaacgcgcg acgtgtgagt tcaaatcgac gggctgcgag
     9421 gcgttgaagc gatagcccgc tgtgttgagc aatacgaggc cgcccgcgtc gcggaagctg
     9481 tcgccggtcg agccgaaatc gttggcggga tagcggcgcg cggcgtcggc cagcacggcg
     9541 gtcgcaagcg ggttgacgcc gttgagttcg gggaagatcg tgcggatgtt ctccgccgtc
     9601 agcgtcgtca cgccgcccga agcgttctca tagcgcaact cgccgcgccc catgctcggc
     9661 agcggcactc tggagagcac gctcgtctgg ctgcggtcgc ggcgaccttc gaagctgtag
     9721 aagaagaagg cgcggtcttt gatgatgggg ccgccgatgg cgccgccgaa agtgttgcgg
     9781 atgagcgcgg gacgttcgac gccgctgcga ttgttgaaga agtcgttggc ggtgagaatc
     9841 gtggggcggt gaaaccagaa gcccgcgcct ttgatgtcgt tcgagccgcc tttggtgatg
     9901 agcgaaatct gcgcgccgga ggagcgtccc gcctgcgcgt tcgcgttcac ggtcgtgacg
     9961 cgaaactctt cgatggcctc ggcgttcagg cgcagcacgg tgccggtgga cgggtcgttg
    10021 tcgctgctgc tgtccacggt tccgatctgg ttgttctgcg cttcgttgat gtccacgccg
    10081 tcgagcgtga cgttcgactg gtcgctgcgc gcgcccgcca cgtagcccgt gcgtgtgacg
    10141 ccgggttgga gcgtgagcag cgcggcgacg ttgcgcgctt cgagcggcaa ctgcgtgatc
    10201 tgctgcgaga cgaagttgtt gccgagcgtc gcatcctgcg tgttaagcag ggcttcgttc
    10261 gcggtggagg cgaccgtcac cgtctcggag acgctgccta cttcgagcgc gaggctcaat
    10321 tccgtcggct tggcgacgag cgcgcgcacg tcggttacga cgctcttctt gaagttcgcg
    10381 gcttcggctt cgacgcgata ggtgccgggc gggatcaact cgaaagaaaa gacgccgtcg
    10441 tcgctggtcg tcgtcgtgcg gctgaagttg gtgtccgtgt tcagcagcgt gacgcttgcg
    10501 ccggggatga cgttgccctg cgggtcggcc accgtgccgc gcacggaaga ggtgccggtt
    10561 tgcgcgagcg cactgccgca caccattaga gagagaagga atccgcacaa cgcgagggcg
    10621 gcgcggctgc gagcagaaag ctgatctttc atgggagcct ccagtgggtg ttgccgcaac
    10681 ccggccatcg cctgcgacta atgaccggag ttgagaaaat tcgaacggat agcgatgagg
    10741 tctgaattgc taaacgtgaa aacgccggat gagcttcttg cctccccact gagaacaggg
    10801 cggcaactta ttcgtccaac caagtgtcgt gctgataagg atgttgctgt tgcgagtttg
    10861 agcgatggcc ggattcactt tggtcaaccg ctgtcgttgc gctgttgctg ctaagtgacg
    10921 ctcgcaaata aaggaatagt ctgtcgaaat tgtgcaggca acgtgcgttc cactgtgacc
    10981 gccgcgcagg cgtgcggaag taagttgtga gaacggggaa attcctgcgg gcgcgctgcc
    11041 cgacgggagc gcgctctacg aaaaattaaa gagtgcgatt caaaaatttg gatcaacgcg
    11101 aaagagtcgc gcgctgtaga tcggcggagg aatatatgtc gagggatgca agcacggctg
    11161 caagcacggc tcgcttcgtc tgcaaagctt atctcgcgtg cctgcttcga gagacgatga
    11221 gctacttgat gctctcccgt tcgatgttct caagcaggtg gcctaacttc tctttcttcg
    11281 tgcgcatgta atgtgcggcg gcttcggtgt gcggcacctg aatcgacacg cgctcgacca
    11341 cctgcaagcc cgcctcttcc atcgcgcgca gcttcagagg attgttcgag agcaggcgca
    11401 cgcgacacaa accgagatcg aagagcacct ctgcgcactg gcgatactcg cgcaggtcta
    11461 cggcgaggcc gagtcgttcg ttggcttcca cggtgtctgc gccgttgtct tgcagggcgt
    11521 aagcgcgaat cttgttgatg atgccgatgc cgcgcccctc ttgcagttga tagacgattg
    11581 cgccgcgtcc ctcctgctcg atcatacgca tcgcctgatg gagttgcgcg ccgcaatcgc
    11641 atttgagcga accgaacacg tcgcccgtga ggcactgcga atggatgcgc gcgagcgtcg
    11701 gcgtctcgcg ctcgatctcg cctttgaaga gcgcgacaaa ctcctcgccc gattcgggcg
    11761 cgcgataacc cgcgatctta aattcgccga acgcggtcgg caggttcgcc accgacacgc
    11821 gctcgaccga gaggttggtc gtcgcccact ggcaagtggt gctggtctgc tgcggtgttt
    11881 cttccaaaac agcttactcc cggagacaac tggcaaaact tcgcgcccgt ctcctccgct
    11941 taaagcgatg agacgacgat ttagccgaaa cgtttcaact gtcctgaatt ataagttccc
    12001 gtgccggaaa gcgacaagac gacagcagga agccgcgtcg cataaaaaca tcaccccgcc
    12061 ggttaaaagt tgatcttttc tttttcatcg cgcatccttt ctgcgttcgc gctccctacg
    12121 tctatggaag aacaaaactt tggagaaata acgctccccg ccgacccgcc cacgtcgcac
    12181 gtgcgccgcc ccgcgctcgt cttcatgcgc ggcgaacaac tggccgcgcc catcccgctc
    12241 gaacgcgacg aggtcatact cggtcgcgcg ctcgaagcag acgtgcgtgt caacgatgcc
    12301 cgcgcctcgc gcctgcacgc gcgcatcagc accgaactcg acgccgagac gaacacctct
    12361 cgctatcgtc tcaccgatct cggctcaacc aacggcacgc tcctgaacgg ccagcccgtc
    12421 accgaagcct tcctccagaa cggcgacaaa ctgatcatcg gcgagcacct catccgcttc
    12481 gacctgctcg acgatctcga ccgcgagttt caacgtcaac tctatcgtct catcgcgcac
    12541 gacgaactga ccggactcct gacgagcaag tctttcttct ccgaactgcg ccgcgaagcc
    12601 gcgcgtgcgg agagcgaagg ccgcccgttc tgcgtcctga tgatggattt ggatcatttc
    12661 aaacaggtga acgacacctg cggccacctc gtcggcagcc agacgctcga agagatcggc
    12721 ggcctcatca ctcgcgcact gcgcgcgggc gacgtggccg cacgcttcgg cggcgaagag
    12781 tttgccgcct tcctcctcga cgccgattgc gcgcaagccc tcgtcgccgc cgaacgcgtc
    12841 cgcaccgcga ttgaagagca cgctttttcc tccacccgcc acggcacgaa cgagaaccgc
    12901 acgctccata tcacgatcag catcggcgtc gccgcctacc cggacgacgc gcgcgatccg
    12961 attgaactga tcgaactggc cgacaccgcg ctctaccacg ccaaacagtc cgggcgaaat
    13021 cgcgtcagcg cgttttgcct cgccccgtcc gacgatgaga cgccgcgcga acttcgcccg
    13081 cgcaacgccg tgctgccgcg cgatgagtga gtagtgttcc ggtttcagtt ttcctccgtg
    13141 cccgctctgg tgccctctgt agtctctgtg gtgagtctta tgaataagca ttcaccataa
    13201 agacacagag ggcaccagag cgggcacaga gacagggcaa ttgagaacac tacgagcaag
    13261 tgataaaagt tgacggcgag caaaaccttc gtggtatgat gccgccactc aagcaagccc
    13321 ccgtcgttcc ttagtacccc cacccgaagt cttaatactt gcgacgaatt ttgtcgcacg
    13381 cttcaaaagg atcaagagcg tgttgaagtt caccttcgtt ccgcgcgtcg cctgcggcct
    13441 cgccgtcgca ctcttcatct acggcctcgc cgcgacggct gcgcccgcgc aggcgcaggc
    13501 cacgcaaccg gcgcagaggc gagcagttgc gcccgtcacc acatcacagg ctccggcaca
    13561 gattcctgcg ccgtcgccga ctcctgccgc gactgcgacc gtagcgacgg acaatcacga
    13621 tctctcgatc aacgcaaacg tcactgcgcg cgagttgcac ttcgaagttg tgccgaaccc
    13681 gaccgtggag tttccggggc agccgcgccg cgataccgtg tgggaagcgg agcgcacgaa
    13741 tctccccgcg caagttcagc ccggcgtcac ctatcgagac atcggcatcc gtctcaagat
    13801 cacgagcgtc ttcgcggaca tcgaacgcat cgtcgccgaa gccctcggcg aacttcccgc
    13861 gcgcgacggc gacaacaatc cgccgacgcc tgagccgaac ccgaacgacg cgccgccgca
    13921 aattgagaac gcgccgcaag ccacgccggg cgcgacgcaa acgaacgccg cgcctcaagc
    13981 ggcctccgca ccaatccaag cggcagcttc gacgcaaact ccgcgcgccc cattcgcgca
    14041 gacaacttcc gcgccgcaga ccgctccgac gcgccagaag agcagcagcc acgcgaccgt
    14101 gcgacggggg agacatcaat gaatcgggtt gttcgccgga tcgctctggc cgcgctcttc
    14161 tgcgctgccc tctgtatcgc cgtcgccgca caacagacgc cgccacgcga tgcgacagtc
    14221 accaacccgc ccacgcggcg caccacgcct ccgttgtctc ccggcgcgca gaacgcggga
    14281 gccgcaaacg tcgaagagaa ttttgaattg aacatcgccg agcggcgcat caccgaacag
    14341 gacttcttcg cctccaccgc gattgagacg gaagcgggcg aggcgcacgg tttgtctctg
    14401 cgcgtcggcg tcgccgtcgg cgcgagcgag atcgacgtgc tcctgcgcaa cgtgcgcggc
    14461 agcgtgcgct ttcgcggctc gctcgatgcc ctgcgtcgtg tcctcgacgc gcgccgcgag
    14521 acgaacgccg ccccgccgcc cgcaggcgtc ccttgaactc tttttaactc tcccgcaacg
    14581 tttgtgagat ggacggagcc ttatcccgac ctttcgctcg aaagctgacg ctcgtattca
    14641 tcaaactttt tctgaggaga acaacgcatg aacaagctct cgtctctcgg agccataatc
    14701 gtcgccatct gtgtcgcgtg cctcgccccg gttgcgacgc aggcacagac gcccagcccg
    14761 tccgccagca ccaccactgc gcgcggcact gacaacgcct cgatcttcgc caacagcacg
    14821 agccaacagg cgcaaccgcg cccgacgccc tcgcccatcg cgcccgtgcg cccgctctcg
    14881 caagaggaca ttcagaggca gcaggccgcg agcgagcgtc gccgccagtc agccgcttcg
    14941 ggcgacccgg atgtgctgct cgacgtgccg aacctctcgg tcgaagagat cacgttggaa
    15001 gtggagaacc tgcgcgcgaa agtttcgctc gatgcgcgcc tggcgaacct cttgcagttg
    15061 agcgcaggcg cggacgccgg gattgataaa gtcaaactca cgatcaaagg cgtgcgcgca
    15121 gagttgttgt tgaaggtgcg gctcgacaac gt
//
LOCUS       DASWNS010000664         8976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_466868, whole genome shotgun sequence.
ACCESSION   DASWNS010000664 DASWNS010000000
VERSION     DASWNS010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466868"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6410)
                     /locus_tag="VGB76_22970"
     CDS             complement(<1..6410)
                     /locus_tag="VGB76_22970"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0101807.1"
                     /translation="MFGLRRFAFIALALLLCSVLLPRMTGAEWSEDSQAVEAGKEAKA
                     QTLRAEFVPGEVLVRFRQDSTGGKSTTPGMLMLQRAEARAIELRVEPFAVSESVEGLR
                     LARVEPEETLAAVDALNARPDVVYAEPNYIWRKAALPNDPRFSELSGLRNLSHPTADI
                     DAEQAWDTTTGSRNVVVGVIDEGIDINHEDLRDNIWRNAAEAGGAPGFDDDGNGYVDD
                     INGWDFHHNDRTVYDGPGTNSDGSPVDAHGTHVAGTIGASGNNAKGVAGVNWQVSLLP
                     LKFLGANGVGSTADAIRAYDYARAMRQRWDASGGAQGANIRVLNNSYGGRGYSQAAYD
                     AIRALGDAGILFVTSAGNEARNNDRFPSYPTNYQLPNLVSVGSTGSSGLPSTQFSNYG
                     ERTVDLVAPGENILSTTPGNSYDKYYGTSMAAPHASGTAALVCAAYPDISLRRLRAAL
                     VYGGSQGFAQFTSSAKRVNARGSLDNAAETDTNAPAALADLSISSQGNQKFALRWTAP
                     GDDGTGGARVAVYEVRYSDAHPATPGVFDTAYTLVAPTPAAPGSIQTASVTVPYRHAA
                     GFIGVRAIDNAGNAAPLAVVNLNAEIAVADPYTVSVAGAGEGLSTGGTPLNLRADDAY
                     TTYQLPFEFTFFGRRTGGVTISTNGAIHFTYPSQLPDGSPDIDVNGTDYLSARQIIAG
                     VWDDLRTDRRAGDDVYVSQPDPERVIFRWQAVTFNYTHGAGAGRGENPVNFEIELRRD
                     GTFLIRYGAGNHNLLPVVGASGGEPDPYIVASHTSEFALKDLAFAPVVTFAPRRPTPP
                     PAPDLAVSMRITPDPAASGQQLIYQIEATNRSFDQTAERVSVVSQLPQGVSFISCAAP
                     YQSVCTAPAVGSTGAVTLSLNRLSPISTASISIVVQVNAPPGSTLHNTTTITNYWNDP
                     NPSNNTATVSTVVQDASVFAGVRAISAGGSSNGGHTLAVKSNGTVWAWGLNVAGQLGD
                     GFNYLDSHMPVQVVGLSDVSAVDAGSDHSLALKRDGTIWAWGNNSYGQGGAYSQFNSN
                     RTRPAQVSGLSGAFNALAAGNAHSLALRSDGTVWAWGSNASGQLGNGGTYAHAVYEPV
                     QVAGLTNVTAIAAGSAFSLAVKSDGTVWAWGANLSGVIGKSSETFSVGAPVQVAGIND
                     VSSVSAGERHALALKRDGTVWAWGDNVDGNLGSGNISSTPTPRQVGGLSNVKEINAGS
                     AHSVVVKTDGTVWSWGRNSGGALGDGTMTTRFVPVQVGGLNGVTTVASGHDHSGALLA
                     DGTVRMWGGNIRGQLGDRTYVSRTTPTDVSGPYGVATPVFSPDGGAFNSTQGVIIGCA
                     TQGVFIHYTTNGAEPTENDPYVTSNSTIYIERTLTLKAKAFKHGWPASAVKSANFTLP
                     TVPPTPSPTPVPGAGNQPIAFSRWFSTSGSDIFLMNPDGTGEVNLTNRQGSDYDPAWS
                     PDGKKIAFVTLRGFDGKTKIYVMNADGTNPQNLSYTSNEDSAPVWSPDGGQIAFVSFG
                     VNFPSRIYLMNADGTGRRPLSDMEYVGTPSWSPDGQHIIFTALGSSSEIWAARVDGSS
                     VQRLTNNTWSEQMPAYSPDGKRIAFVSDRHGDIYNNEIYVMNADGTNPVRLTNSPGWD
                     GSPSWSPDGSKIIFDSQRDGAMTSKIYVMNADGTNQIRVTGDTTSDGGAAWRPQVSPL
                     VQFESAAYSVSEGAGTANVTVTRKGDVSGIVSVDYATTDETASASSDYNAASGTLTFN
                     GGETTKTFSVQILEDALAEGRETLGLRLSNPNGGAELGSPATATLNINDNEPCSNSIS
                     PSSHTSPAPGETLTVNVNALGGCDWTAVSNSNFINISSGASGSGNGVVKLAILPNGSG
                     VPRVGTATIAGQTLTVTQPELVPEVSILQFSQPSYQFGEAAGSATLMVTRLGDKTGTA
                     TVQYQTIDDPAAVPCDPQLKRPDGSSYPQGAAYARCDYATSVDTLTFLPGQESAQLHI
                     PLIDDAYAEGEESVQVRLFTPAGATLPAASGATATLLIQDNDATTGTENPARTTAFFV
                     RMHYLDFLSREPEAGEPWSAILNNCANAFNLDANNPSAACDRLIVSQSFFGSPEFRMK
                     GFYVYNFYRVAFGRLPEYAEIIPDMRSVTGQNAAEVYQKRAALPLRFMERAEFKTRYD
                     ALSDT"
     gene            complement(6664..7167)
                     /locus_tag="VGB76_22975"
     CDS             complement(6664..7167)
                     /locus_tag="VGB76_22975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101808.1"
                     /translation="MYCQTCAAEIQPGLNYCNRCGGVVNASLASRAETPAALVDLKSP
                     VRTIGAAVTLTTLIGITIIFVALDGLSRGALPPELLGMMGAAGFFLILVIDVLLIRIL
                     SRLVQLPVAPPALPPQSRRPEARELHAPPAQTYMTAATDPLPASSVTDHTTRTFHPVY
                     KEPQAKS"
     gene            complement(7264..7461)
                     /locus_tag="VGB76_22980"
     CDS             complement(7264..7461)
                     /locus_tag="VGB76_22980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101809.1"
                     /translation="MWRVIGIIALGLVVLVIGVPLLLAAAGIVLGGIGMIIGLAVAVI
                     KLAVVVAIGYLALVAIRALMR"
     gene            7753..>8976
                     /locus_tag="VGB76_22985"
     CDS             7753..>8976
                     /locus_tag="VGB76_22985"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY0101810.1"
                     /translation="MPRRKTDTLFDEPEPHVAAEAATDAPLAERMRPRTLEEFVGQRH
                     LIGEGRILRRLIEGEGALPSLILWGAPGTGKTTLARLLARHSEAEFIPLSAVFSGVKD
                     LRAAIAEARDTRRTGRRTVLFVDEIHRFNKAQQDALLPAVEDGTVTLIGATTENPSFE
                     VISALLSRSRVLSLEPLNEDDLAALIRRALTDEARGLANLHPQISDEVLQRFARAAGG
                     DARVALTGLEAAVESTAPDERGVRHITDEIVVEALGRARYAYDKGGEEHYNLASALIK
                     SLRNSDANAALYWLARLIEGGADPLFIARRLCILASEDVGLADPQAIVQAAAAAQITA
                     LIGLPEALYPLSQATIYLARAPKSNSVKRAYFAAAEDAQETAREPVPLHLRNAVTPLM
                     KRSGYGSGYRYVHDDP"
BASE COUNT         1617 a         2810 c         2881 g         1668 t
ORIGIN      
        1 gtgtccgaca gcgcatcgta gcgcgtcttg aactccgcac gctccatgaa gcggagcggc
       61 agcgcagcgc gcttctggta gacctcggct gcgttctgcc ccgttacgct gcgcatgtcc
      121 ggaatgatct ccgcatactc cggcaacctg ccgaatgcca cccggtagaa gttgtagaca
      181 tagaagccct tcatgcgaaa ctccggcgag ccgaagaacg attgcgacac gatcaggcgg
      241 tcgcacgccg ccgaagggtt gttcgcatcg agattgaaag cgttcgcgca gttgttcagg
      301 attgccgacc acggttcgcc cgcttccggc tcgcgcgaga gaaagtccag atagtgcatg
      361 cggacgaaga aggcggtcgt gcgcgcaggg ttctctgtgc cggtcgtcgc atcattatcc
      421 tgaatgagca gcgtggcggt cgcaccgctc gcagcaggca gcgtcgctcc ggcgggagtg
      481 aagaggcgca cttgcacgct ctcctcgcct tcggcgtaag cgtcgtcgat gagcgggatg
      541 tggagttgtg cgctctcctg accggggagg aaggtgagcg tatcgacgga ggtggcgtag
      601 tcgcagcgtg cgtaggctgc gccctgcggg taggaggagc catcgggacg tttcaactgc
      661 gggtcgcagg gcacggcggc agggtcgtca atggtttgat actggactgt cgccgttccg
      721 gtcttgtctc ccaaccgcgt caccatgagc gtcgcactac ccgccgcttc gccgaactga
      781 taacttggct ggctgaactg caagatggag acttcgggaa ccagttccgg ctgtgtgacg
      841 gtcaaggtct gtccggcaat agtcgccgtg ccgactcgcg gcacgccgga gccgttcggg
      901 agaattgcga gtttgacgac tccgttgccg ctgccgctcg cgccggaact gatgttgatg
      961 aagttgctgt tactaaccgc cgtccaatcg cagccgccga gcgcgttgac gttcactgtg
     1021 agcgtttcgc ccggagcggg cgaggtgtgg ctcgaagggc tgatcgagtt ggaacaaggc
     1081 tcgttatcgt tgatgttgag cgtggccgtg gcggggctgc ccagttccgc gccgccgttc
     1141 gggttgctca aacgcaggcc gagcgtttcg cgcccctccg cgagcgcgtc ttcgagaatc
     1201 tggacggaga aagttttcgt cgtttcgccg ccgttaaaag tgagcgtgcc gctcgccgcg
     1261 ttgtagtcgg aagaggctga ggcggtttcg tcggtcgtgg cgtagtcaac gctcacgatg
     1321 ccgctcacgt cgcccttgcg cgtgacggtg acgttggccg tgcccgcgcc ctccgagacg
     1381 ctataggctg cgctctcgaa ttgaaccaac ggagaaacct gtggacgcca cgcagccccg
     1441 ccgtccgatg tcgtatcgcc tgtgacgcga atctgattcg tgccgtcggc gttcatcaca
     1501 taaatcttag aggtcatcgc gccgtcgcgc tggctgtcga agatgatctt cgagccgtcg
     1561 ggcgaccatg agggcgagcc gtcccagccg ggactgttcg tgagccgcac gggattcgtg
     1621 ccgtctgcgt tcatcacgta gatttcgttg ttgtagatgt cgccgtgccg gtcgctgacg
     1681 aaggcgatcc gcttgccgtc gggcgaatag gcgggcatct gttcgctcca cgtattgttg
     1741 gtgagacgct ggacggaact gccgtccacg cgcgccgccc agatttcgct cgaactgccc
     1801 aacgcagtaa agattatgtg ctgcccgtcg ggcgaccaac tcggagtccc gacatactcc
     1861 atgtcgctca acggcctgcg ccccgtgccg tctgcattca tcaagtagat acgggacggg
     1921 aaattgacgc cgaagctgac aaacgcgatc tgcccgccat cgggcgacca gaccggcgcg
     1981 ctatcctcgt ttgaggtgta gctcaagttt tgcgggttgg tgccgtcggc gttcatcacg
     2041 taaatcttgg tcttgccgtc gaagcccctg agcgtgacga aagcgatctt cttcccgtcg
     2101 ggcgaccacg ccggatcata gtcgctgccc tgcctgttgg tgagattaac ttcacccgtg
     2161 ccgtccgggt tcatcaggaa aatatcggag ccgctcgttg agaaccagcg cgaaaaggcg
     2221 atgggctgat tgcctgcacc gggaacgggc gtcgggcttg gcgtcggcgg cacggtgggc
     2281 aaggtgaaat tcgcgctctt caccgcgctc gcaggccagc catgtttgaa ggctttcgcc
     2341 ttgagcgtca gcgtgcgctc gatatagatg gtggagttcg atgtgacgta gggatcgttc
     2401 tcggtcggct cggctccgtt ggtcgtatag tggatgaaga cgccttgcgt ggcgcacccg
     2461 atgatcacgc cctgcgtgga attgaacgcg ccgccgtcag gtgagaagac gggcgttgcg
     2521 acgccgtaag gaccactgac atcggtgggc gtggtgcgcg agacataggt gcggtcgccg
     2581 agttgtcccc tgatgttgcc gccccacatg cgaaccgtgc cgtctgcgag cagcgcgccg
     2641 ctgtgatcgt ggccgctggc aaccgtcgta acgccgttca atccgccgac ctgcacgggc
     2701 acaaaacggg tggtcatcgt gccgtcgccg agcgcgccgc cgctatttct tccccaactc
     2761 cagaccgtgc cgtccgtctt cacgacgacc gaatgagcgg agcctgcgtt gatctctttc
     2821 acgttggaga ggccgccgac ctgtctgggc gtcggagtcg agctaatgtt tccgctgccg
     2881 aggttgccgt caacgttatc gccccaagcc cagaccgtgc cgtcgcgctt caaggccagc
     2941 gcatgccgct cccctgccga gacggacgag acatcgttga tccccgcaac ctgaaccggt
     3001 gccccgacgc tgaacgtctc ggacgacttg ccgatgacgc cgctcaggtt cgcgccccaa
     3061 gcccagaccg tgccgtcaga cttgaccgcc agcgagaacg cgctgcccgc cgcaatggct
     3121 gttacgttcg tcagccctgc gacctgcacc ggctcgtaga cggcgtgggc gtatgtgccg
     3181 ccgttgccga gttgacccga agcgttgctg ccccaagccc agaccgtgcc gtcagacctc
     3241 agtgcaagcg agtgtgcgtt gcccgccgcg agagcattga atgcgccgct caggccggag
     3301 acttgcgccg gtcgtgtgcg gttggagttg aactggctgt aggctccgcc ctgcccgtag
     3361 gagttgttgc cccaagccca gatcgtgccg tcgcgcttca aggccagaga gtggtcgctt
     3421 ccggcatcca ccgccgacac gtcgctcagc cctacgacct gcaccggcat gtggctgtcg
     3481 agatagttga agccgtcgcc gagttgcccg gcgacgttca agccccaagc ccagaccgtg
     3541 ccgttggact tgaccgcgag cgtgtgcccg ccgtttgaac tgccgcccgc agagatggcg
     3601 cgcacgcctg cgaagacgga ggcatcctgc accaccgtcg agacggtcgc cgtgttgttg
     3661 gaagggttcg ggtcgttcca gtagttcgtg atcgtcgtcg tgttgtgcag agtgctgccg
     3721 ggcggcgcgt tgacctgtac gacgatagag atcgaggcgg tgctgatagg cgagagacgg
     3781 ttgaggctca acgtgaccgc cccggtcgag ccgacagcgg gcgcggtaca gacggattga
     3841 tagggtgccg cgcaggagat gaacgagacg ccctgcggca gttggctcac cacagacacg
     3901 cgctcggcgg tctgatcaaa actccggttg gtggcctcta tctggtagat caattgctgc
     3961 ccggaggccg ccgggtcggg tgtgatgcgc atactcacgg cgaggtcggg cgcgggcgga
     4021 ggagtcgggc ggcgcggcgc gaaggtgacg acgggcgcga aggccaagtc cttcaacgcg
     4081 aactccgatg tatgtgatgc gacgatatac gggtcaggct cgccgcccga tgcgccgacg
     4141 acgggcagca ggttgtgatt gcccgcgccg tagcgtatga ggaacgtgcc gtcgcgccgc
     4201 aactcgatct cgaagttgac gggattctcg ccgcgtcccg cgcccgcccc gtgcgtgtag
     4261 ttaaaggtga cggcctgcca gcggaagatg acgcgctccg gatcaggttg cgagacatac
     4321 acgtcgtcgc ccgcgcgacg gtctgtgcgc aggtcgtccc agacgcccgc gatgatctgg
     4381 cgcgcgctca ggtagtccgt cccgttcacg tcaatgtcgg gcgagccgtc cggcaattgc
     4441 gacgggtagg tgaaatggat cgcgccgttg gtcgagatgg tgacgccgcc cgtgcgtcgt
     4501 ccgaagaagg taaactcgaa gggcagttga taagtggtgt aggcgtcgtc ggcgcgcaag
     4561 ttgagcggcg tgccccctgt gctgagtcct tcgcccgccc ccgcgaccga caccgtgtaa
     4621 gggtctgcga cggcgatctc cgcgttgaga ttgacgacgg cgagcggagc ggcgttcccc
     4681 gcgttgtcta tcgcgcggac gccgatgaag cccgccgcgt gacgataagg caccgtgacg
     4741 ctcgcggtct gaatcgaacc gggcgcggcg ggcgtcgggg cgacgagcgt gtaggccgta
     4801 tcgaagacgc ccggcgtggc gggatgcgcg tccgaatagc gcacctcgta gacggcgacg
     4861 cgcgcgccgc ccgttccgtc gtcgcccggc gcagtccagc gcaaagcgaa cttctggttg
     4921 ccctgactgc tgatgctcag gtctgcgagc gcggcgggcg cgttcgtgtc agtctcggcg
     4981 gcgttatcga gggagccgcg cgcgttcaca cgcttcgcgg aagaggtgaa ctgcgcgaag
     5041 ccctgcgagc ctccgtagac gagcgcggcg cgcagacgac gcaacgaaat atccgggtag
     5101 gccgcacaga cgagcgcggc tgtgccggag gcatgcggcg cggccatcga agtgccgtaa
     5161 tacttatcgt agctgttgcc cggcgtggta ctgagaatat tctcgccggg cgcgacgaga
     5221 tcaaccgtgc gttccccgta gttggaaaac tgcgtggagg gcagcccgga cgagcctgtc
     5281 gagccgaccg agactaagtt gggaagttga taattggtcg ggtaagacgg aaagcggtcg
     5341 ttgttgcgcg cttcgttgcc cgccgaagtg acgaacagga tgcccgcatc gccgagcgca
     5401 cgaatggcgt cgtaggccgc ctgcgaatag ccgcgcccgc cgtagctgtt gttgagcacg
     5461 cgtatgttcg cgccctgcgc gccgccggag gcgtcccaac gctgccgcat cgcacgcgcg
     5521 tagtcgtaag cacgaatggc gtcggcagtt gagccgacgc cgtttgcgcc taaaaacttc
     5581 agcggcaaga gactgacttg ccagttgacg cccgccacgc ccttcgcgtt attgccggag
     5641 gctccaatcg tcccggcgac gtgcgttccg tgtgcgtcca cgggtgaacc gtcggagttc
     5701 gtgcccggcc cgtcatagac cgtgcggtcg ttgtgatgaa agtcccagcc gttgatgtcg
     5761 tcaacgtagc cgttgccgtc gtcatcgaaa cccggcgcgc cgcccgcctc cgccgcgttg
     5821 cgccagatgt tgtcgcgcaa gtcttcatga ttgatgtcaa tgccttcgtc aatcacgccg
     5881 acgacgacgt tgcggcttcc ggtcgtcgtg tcccaagcct gttccgcatc aatgtcggcg
     5941 gtggggtggg agaggtttct gaggccggac aactcggaaa agcgcgggtc gttgggcagt
     6001 gcggctttgc gccagatgta gttcggctcg gcgtagacga catccggcct cgcgttgagc
     6061 gcgtcaaccg ccgccagcgt ctcttcgggt tcgacgcgcg ccagacgcaa gccttccacg
     6121 gattcggaga cggcgaacgg ctcgacgcgc aattcaatcg cgcgcgcctc tgcgcgttgg
     6181 agcatcaaca tccccggcgt cgtgcttttg ccgcctgtcg aatcctgtcg gaaacgcacc
     6241 agcacctcgc ccggcacaaa ctccgcacgc agagtctgcg cttttgcttc tttgcccgct
     6301 tccacagcct gcgagtcttc agaccactcc gcgcccgtca tgcgcgggag caacacggaa
     6361 cagagcagca acgccagcgc gatgaacgcg aagcggcgta aaccaaacac acgtcgaacc
     6421 tttgttttta atccttgaga cgccaacgaa ctgctcctgt tgagtatgga taacggacgg
     6481 agtcaattat agatgtaaaa aacggctctc cggttggccg cgccgcaggg tgcggaaacg
     6541 cgggaaagcc gccttcaacg gaaactcatt ttatctcgtc ggcaggaaag atcaaccgtc
     6601 gcgggggttc gcaggcggct gctccgtgcg cgcggcgcgg cggctgtcct gacgcgggag
     6661 aggtcagctt ttcgcttgcg gttctttata gacgggatga aaagttcgcg tcgtgtggtc
     6721 ggtgacgctc gacgccggga gcgggtcggt tgcagcggtc atgtaagtct gcgcgggcgg
     6781 cgcatggagt tccctcgctt cgggtcgcct cgattgaggc gggagagcgg ggggcgcgac
     6841 gggcagttgg acgaggcgcg agaggatgcg aatcagcaga acgtcgatga ctaagatcag
     6901 gaaaaatccg gcggctccca tcatccccaa cagttcgggc ggcagcgcgc ctctggaaag
     6961 gccgtccaaa gcgacaaaga tgatcgtgat gccgatgagg gtcgtcagcg tgacggctgc
     7021 gccgatggta cgcacggggc ttttcagatc gacgagcgca gctggagtct ccgctctgga
     7081 cgccagagat gcgttcacca cgccgccgca ccgattgcaa tagttgagtc ccggttgaat
     7141 ttcagcggcg cacgtctggc aatacatagg ctctccgagg cgtaaacttt aagctgataa
     7201 aacgaagcgc ccgttcgaag gtggacgaag gaacgggcgc ttgcttggta aatttcgcaa
     7261 ccttcagcgc atcaaagcgc ggatggcgac gagtgctaaa taaccgatgg cgacaacgac
     7321 ggcgagtttg atcacggcca ctgccaatcc gatgatcatg ccgatgccgc cgagcacgat
     7381 gccagccgcc gccaataaaa gcggcacgcc gatcaccagc acgactaaac cgagtgcgat
     7441 tattccgata actctccaca tatcattcat ctcatccacc tcaacttccg tctcctgaac
     7501 taagacgcgc cgggctggaa aagtctggaa attttctttc cgtccctgcg ctcgccctca
     7561 tatacgaaca accctcgacg aacgttccat ccaagactgc ggatgcgacc gcctgcatcg
     7621 cgctccccaa catgcgctga aattatgtta agctatgata acaaatctcc cggtatgcgc
     7681 cagaattttt tacgccggaa actgccttcg caggcggttt tcatagccga cgttcgacag
     7741 gaattttaaa cgatgccgcg ccgcaaaacc gacactttgt ttgacgaacc cgagccgcac
     7801 gtcgccgccg aagccgccac cgatgctccg ctcgcggagc ggatgcggcc acgcacgctc
     7861 gaagaatttg tcggtcaacg ccatctgatc ggcgagggtc gaatccttcg ccgcctgatc
     7921 gaaggcgagg gcgcgctgcc gtcgctcatt ctctggggcg cgcccggcac gggcaaaacg
     7981 acgctcgcgc gcctgctcgc acgccacagc gaagcggagt tcatcccgct ctcggcagtc
     8041 ttctccggcg tcaaagattt gcgcgcggcc atcgccgaag cccgcgacac acggcgcacc
     8101 ggacgccgca cagtgctctt cgtagacgag attcaccgtt tcaacaaagc gcagcaggac
     8161 gcgctgttgc ccgccgtcga agacggcacg gtaacgctca tcggcgcgac gacagagaac
     8221 ccttcttttg aagtgatcag cgcattgctc tcacgctcgc gcgtcctctc gctcgaacct
     8281 ctgaacgaag atgacctcgc cgcgctcatc cgtcgcgcgc tcacggacga agcgagaggt
     8341 ctggcaaacc tgcacccgca aatttccgac gaagtgctac agcgtttcgc acgcgcggcg
     8401 ggcggcgatg cgcgcgtcgc gctgacaggg ctggaagcgg cggtcgaatc aaccgcgccc
     8461 gacgaacggg gcgtgcgcca catcaccgac gagatcgtcg tcgaagctct ggggcgtgcc
     8521 cgttacgctt acgacaaagg cggcgaggag cactacaatc tcgcctccgc cctgatcaaa
     8581 tccctgcgca actccgacgc gaacgccgcg ctctactggc tcgcgcgcct catcgaaggc
     8641 ggagccgacc cgctcttcat cgcgcgccgc ctctgtatcc tcgcctcgga agacgtgggc
     8701 ttggccgatc cgcaggccat cgtgcaggcc gccgccgccg cgcagatcac cgcgctcatc
     8761 ggcctccccg aagctctcta cccgctctcg caggcgacga tctatctggc acgcgcgccc
     8821 aagtccaact ccgtcaaacg cgcctacttc gccgccgccg aagacgcgca ggaaaccgcc
     8881 cgcgaaccgg tgccgctgca cctgcgcaac gccgtcacac cgctgatgaa acgctccggt
     8941 tacggcagcg gttatcgcta cgtccacgac gacccg
//
LOCUS       DASWNS010000665        12497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_336256, whole genome shotgun sequence.
ACCESSION   DASWNS010000665 DASWNS010000000
VERSION     DASWNS010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12497
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336256"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3305)
                     /locus_tag="VGB76_22990"
     CDS             complement(<1..3305)
                     /locus_tag="VGB76_22990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101811.1"
                     /translation="MKGHRRSGAWSKLVSSCLLLVGLIVLASSPALAQQLSARQHFSL
                     SATRLPGDTNAELSRTGDAVPAKVNASDPSVTPSNATATTPEATGAPAANEAGSPASG
                     TVTAAAPTTATTTTTTAAAAAPITTAAALTAAITAAAVQQTAAIPQCSRTVTANVVAL
                     DQLYTYNRFGAFNPAGMMYALKRDVTVIDATKGFVAGNVRLKPGKRPRPLVLRANEGD
                     CLTVNFTNLLTPTRDGLDAINAANGTSRLAYFDKPPFFIPKEGTISYDLSQNKLRTLT
                     NGQVNVIDENGNVIQDGNIKMQKNDTPATRAASMHVNGLEYLSIASDGANVGNNASSL
                     AAPGSTVTYKWYAEKQGQFFMYSMGTVSGGEADGGQLDLGLFGSINVQPRGSKWYRSQ
                     VTAAQLTAVKTGTNPNGTPIINYDKLGTDNQPILGMLNASNEIIYSDLNAIISNFGEN
                     CENAPPSGTCGEAFREFTVIFHDETKTVQAFPELNQELFHGVRDGFAINYGSGGLGAE
                     ILAVKKKVGPAANCPDCAFEEFFLESWANGDPAMNVRKDSTGKAVEALYPDDPSNVHH
                     SYMGDPVRFRNLHAGPKETHVFHLHAHQWLHESREDGSTYLDSQTIGPGGAFTYEINY
                     GGSGNRNLTVGDSIFHCHLYPHFAQGMWELWRAHDVFESGTPDRNLPDAEIAGGTPNP
                     AIIPLPNKPMPPMPTAEFKGYPFYIAAQPGHRAPQPPLDMDHDGGLPRHRILEATHVS
                     GAAAIPAAKLNDPVAARVLSLNKDPNLLAFAHKLTSAKLEILPQTGTPAEKIAMNFHQ
                     GLFAGGAPATTSFGWDGAAYPSYTATGVAGKFFVNGRAPKPGAPFADPCPTNFTDGAG
                     ALKAVPNRNYRAAYVQFDMTVNRAGWHDRQARIMVLEQDVANTLNGTRPPEPLFFRAN
                     SGDCITFKATNLVPGALNLDDFQIYTPTDIIGQHIHLVKFDVTSSDGAGNGWNYEDGT
                     FAPDIVRERIEANNKYQQSIGGTQILTPKTHPAFGAGPNNEWIGAQTTVQRWWADPLV
                     NRAGEDRTIRTVFTHDHFGPSSHQQHGLYAALVVEPTDSTWLAFDGTTLGTRPDGGPT
                     SYKANILFDTPYT"
     gene            complement(3410..6322)
                     /locus_tag="VGB76_22995"
     CDS             complement(3410..6322)
                     /locus_tag="VGB76_22995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY0101812.1"
                     /translation="MKRLAALLLIIGVCAIALVPSHTQAQRPEVSQGKLRRNAAAIKN
                     QYIVVFNESVRREQVGEEAARLVRLHGGTIGFTYRNALRGFSVEMNEAAAEALSRHPK
                     VEFIEEDAEVQGASTQTNAPWQLDRLDQRTPAPSNTYTYEANGAGVNAYIIDGGIRYT
                     HQEFGGRAAFAYDYIAGGTGADCNGHGTHVAALLGGNTYGVAKGVKLWSVRVLDCANG
                     GTLARIISGVDWVAGNHVKPAVANLSFITIVSTSVNGTMDIAVKNLIAAGVTTVVAAG
                     NNTGDASLRTPARVPEAITVGATDQNDNRSPNSNFGASVDVFAPGVDIVSAHVTGDAA
                     TSIRSGTSSAAPIVGGLAARYLSTRPGDAPDAVGQAIRNSATPGQVINPGEGSQNLLA
                     YTGITLSDNFNDNVRDARWGVLATPGSNIAEQNGRLEVTPTAAMPGYDGYKSVTAIDL
                     TDTSMSVEAVSVPTTNGFATFYGLADGSGNYLLLGVVSSGTFLMQQSVGGVVTNSQLP
                     YNATQQRFWRFRHNRSDDTVNWETSPDGATWTIHRSIATPFPVTNLQVVLTGQKATAT
                     SPSDTVIFDNLWQEPNPTTYVAMADNFNDNAIHPQMWVVNDPASPTVVSEQNGQLEVT
                     PQPNTSGYNGLEMAGPFDFRDKTLQVEVQPASQAGAVWTYFKLYIDDSNGLVFAVGAN
                     LFTCDSTINGVTDRTQLAWDATIRYWRFRHDIDANTVSFDTSADGVTWTTRKTVTPAF
                     SLGAVRTGFGAGATGTTNAAPGTAIFDNFRVERYRPLFPLSDNFNDNVRAAKWNTLAT
                     SGSNIVEQNGRLEITPAAAMPGYDGYVSATTIDLTDARVAVQTVAVPTTNGFATFYAL
                     RATSGNYLMLGVSTSGTLITQRSVGGVVTQTSVPYNATQQRFWRFRHNRAADTVSWET
                     SPDGATWTTQAAYPTPFDITGLQVLLVGQKATASAPNDTVVFDNLRIERNEGGKAR"
     gene            complement(6445..8484)
                     /locus_tag="VGB76_23000"
     CDS             complement(6445..8484)
                     /locus_tag="VGB76_23000"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0101813.1"
                     /translation="MKKTLALLVIFGAIALVPQLALGQESDSENRLRKNESAIPNNYI
                     VVFNESVPAGRVAAFARQLAREHGGQLGYTYQTALRGFSVEMNEAQAVALSRSPHVAY
                     VEEDAVVEGASVQTNPSWALDRIDQRHLPLDTKYSYANSGAGVNAYIIDGGIRYTHQE
                     FGGRAAFAYDYIAGGTGADCNGHGTHVAALLGGNTYGVAKGVKLWSVRVLDCANGGTL
                     ARIIAGVDWVTANHVKPAVANLSFITIVANSTIDVAVSNMIAAGVTTVVAAGNNTGDA
                     SLRSPARVPEAITVGATDPNDNQSPNSNFGAAVDVFAPGVDIFSAHVTSDTAIAQRSG
                     TSSAAPFVAGMAARYLSGNPQASPASVSKAITSNATPGKIPNIGAGSPNLLLYRPNGK
                     IAFSSYRDGQFEIYSINPDGSEETRLTVHDYSDDYWPEYSPDGTQIAFTSGRGDDLYY
                     QIYVMNADGTNVRRITSNQTGHNAESAWSPDGKQIAFRSSRDGDWEIYVINVDGTNER
                     RLTNSPGADIDPTWSPDGTKIAFPSFRGSPVAYDNIEIFTMNADGSNQVNITNNLGAT
                     GANNDYYPAWSGSGAQLSFVFDGGNAAHTDQIFVMNADGSGRVNLSNNAAYEYNPSWS
                     SDDSRIVFQSGLDDGKGELYIMDADGGNRIRITFNTGNGVDPSTADDFYPDWQAL"
     gene            complement(8552..10708)
                     /locus_tag="VGB76_23005"
     CDS             complement(8552..10708)
                     /locus_tag="VGB76_23005"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0101814.1"
                     /translation="MKFLKVLSLSFCLLALVPQLALGQNLNSQGRLRKSDGAISDSYI
                     VVFNDSVPAARVEAFAAQLAREHRGQLGFTYQNAVLGFSVEMSEAQAIALSHNPHVAY
                     VEEDALVEGAATQTGASWALDRIDQRNLPLDTTYTYLNAGTGANVYVIDGGIRLTHQE
                     FGGRALFAYDNVADGRNGVDCNGHGTHVAGVIGGKTYGVAKDAKLWAVRVLNCLNQGT
                     TARVLAGIDWVAANHVKPAVANLSFITVVGSDTIDLSVRNLIAAGVTTVVAAGNNTGE
                     ASLRSPARVTEAITVAATDQYDNQAPFSNYGAAVDVYAPGVDIISASHFDDVSTSVRS
                     GTSFAAPFVAGSAARYLSANPNETTAAVSLAITSNATPGKVLNAGVGTPNLVLFKPNS
                     KIAFSSMRDGNWELYLMNSDGSNPKNISLHTAKDLPVVWSPDGRKIAFQSNRTGAGDI
                     YVMNSDGSGLTRLTTSTYADQAPTWSPDSQRIAFISKRDGNFDVFVMNADGTNQVNVT
                     RSVSPEYDPMWSPTENKIAFRSFRDGLTKHYVMNADGTNQIRLTAARVDENYMDWSPD
                     GRKIAFASYFYTPSYEEHYDVWVVNADGSSAATNVLPITPYYGAGDFEPDWSPDSKTL
                     VMGSTRYNGSPYGFTELYTINPDGTSPLRLTFSSQEAYGNVAAQWSPGGDRILFMRDA
                     GSNYNYELFIMNADGSNPIRLTTNAAEDYDPVWQPM"
     gene            complement(11429..>12497)
                     /locus_tag="VGB76_23010"
     CDS             complement(11429..>12497)
                     /locus_tag="VGB76_23010"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0101815.1"
                     /translation="AYYYVEKPFTPDEIIMLTERALQFAGIKQENRVLKRTLGEEKEA
                     YGIIGRSPQMRQIYETIRATAPSEASVLIEGESGTGKELIATAFHLQSKRASRPFIRI
                     NCAAFPPDLIESELFGYKKGAFTGADRDKRGLIEAAASGTLLLDEIAEMPAHLQAKLL
                     RVLQERKLRRLGDEQEIPVDFRLVSATNRNTAQATREGLLREDLYFRISTVKITVPPL
                     RERPDDLLPLADHFLQRYAEKYHKPIRGIAQPALSLLMRYDWPGNIRELESVVERAVL
                     FCQEEQLTIESLPEHLNSAGAGQSKCLIPPHLTLEEIEREAIAQTLERAGGNVKKTAQ
                     ILQMHRPTLYRKLKRFKLINN"
BASE COUNT         2135 a         3551 c         4133 g         2678 t
ORIGIN      
        1 aaggtgtacg gcgtatcaaa gaggatgttg gccttgtaag acgtggggcc gccatcagga
       61 cgcgtgccga gcgtcgtgcc gtcgaaggcg agccaggtgg aatcggtcgg ctcgacgacg
      121 agcgcggcgt agaggccgtg ctgctggtgc gaactgggac cgaagtggtc gtgcgtgaag
      181 acggtgcgga tcgtgcgatc ctcgcccgcg cggttgacga gcgggtcagc ccaccaacgc
      241 tggacggtgg tttgcgcgcc gatccactcg ttgttcggac ctgcgccgaa ggccgggtgc
      301 gtcttcggag tcaggatttg cgtgccgccg attgactgct ggtacttgtt gttggcctcg
      361 atgcgttcgc gcacgatgtc gggcgcgaaa gttccgtcct cgtaattcca accgttgccc
      421 gcgccgtcgg acgaagtcac gtcgaacttc acgaggtgga tgtgctggcc gatgatgtcg
      481 gtcggcgtgt agatctggaa gtcgtcgagg ttgagcgcgc cgggcacgag attggtcgcc
      541 ttgaaggtga tgcaatcgcc ggagttggcg cggaagaaga gcggctcggg cggtcgggtg
      601 ccgttcagag tgttcgccac atcctgttcg agcaccatga tgcgcgcctg cctgtcatgc
      661 caaccggcac ggttcacggt catgtcgaac tggacgtagg ccgcgcgata gttgcggttg
      721 ggaacagctt tgagcgcgcc tgcgccgtcg gtgaaattgg tcgggcaggg atcggcgaac
      781 ggtgcgcccg gtttcggcgc gcgtccgttg acgaagaatt tgcctgccac gcccgtcgcc
      841 gtgtaggacg ggtaagccgc gccatcccaa ccgaaggagg tggtggccgg tgcgccgcct
      901 gcgaacaagc cctgatggaa gttcatcgct atcttctcgg cgggcgttcc cgtctgcgga
      961 agaatttcga gtttggccga agtgagcttg tgcgcgaagg ccagcaggtt cgggtctttg
     1021 ttgagcgaga ggacgcgcgc ggcgaccgga tcgttcaatt tggcggcggg gatggcggct
     1081 gcaccgctca cgtgcgtcgc ctcaagaatg cggtggcgcg ggagtccgcc gtcgtggtcc
     1141 atgtcgaggg gcggctgcgg agcgcggtgt ccgggctgcg ccgcgatgta gaacgggtaa
     1201 cctttgaact ctgccgtcgg catcggcggc atcggtttgt tgggaagcgg aatgatcgcc
     1261 gggttgggcg tgccgcccgc gatctctgcg tcgggcaggt tgcgatccgg cgtgcccgat
     1321 tcgaacacgt cgtgagcgcg ccagagttcc cacatgcctt gcgcgaagtg cggatagagg
     1381 tggcagtgga agatcgaatc gccgacggtc aggttgcgat tgccggagcc gccatagttg
     1441 atctcgtagg tgaatgcgcc gccgggtccg atggtttgtg aatcgagata ggttgagccg
     1501 tcttcgcgcg attcgtgcag ccattgatgt gcgtgcaggt ggaagacgtg cgtttctttc
     1561 ggaccggcgt gcaggttgcg gaagcgcaca gggtcgccca tgtaagagtg gtgaacgttg
     1621 gaaggatcgt ccgggtagag agcttcgacc gctttgccgg ttgagtcttt acgaacgttc
     1681 atcgccgggt cgccgttcgc ccacgattcg aggaagaact cttcgaaggc gcaatcggga
     1741 cagttggccg ccggaccaac cttctttttg acggccagaa tttccgcgcc gaggccgccc
     1801 gaaccgtagt tgatggcgaa gccgtcgcgg acgccgtgaa agagttcctg attgagttcg
     1861 gggaacgcct gcacggtctt cgtctcgtcg tggaagatga cggtgaactc gcggaacgct
     1921 tcgccgcaag tgccgctcgg cggcgcgttt tcgcaattct caccaaagtt ggagatgatc
     1981 gcattgagat cgctatagat gatctcgttt gaagcgttga gcatgccgag gatcggctga
     2041 ttgtccgtgc cgagtttgtc gtagttgatg atgggcgtgc cgttcgggtt cgtgcccgtt
     2101 ttcacagccg tcaactgagc cgccgtgact tgcgagcgat accacttgga gccgcgcggc
     2161 tggacgttga tcgagccgaa caggccgaga tcgagttggc cgccgtcggc ctcgccgcca
     2221 gagacagtgc ccatcgaata catgaagaac tgtccctgct tttcggcgta ccacttgtaa
     2281 gtgaccgtcg agccgggcgc ggccagcgaa gaggcgttgt tgcccacgtt cgcgccgtcc
     2341 gaggcgatgc tgagatactc cagaccgttg acgtgcatcg aggcggcgcg cgtcgcgggg
     2401 gtgtcgttct tctgcatctt gatgttgccg tcctgaatga cgttgccgtt ttcgtcaatc
     2461 acgttcacct gaccgttggt gagcgtgcgg agtttgttct gcgagaggtc gtaagagatc
     2521 gtgccttctt tcgggatgaa gaaaggcggc ttgtcgaaat aggccagccg cgacgtgccg
     2581 ttggccgcgt tgatggcgtc aagcccgtcg cgggtcggcg tcaggaggtt ggtgaagttg
     2641 accgtcaggc agtcgccttc gttggcgcgc aggacgagcg ggcgcgggcg tttgcccggt
     2701 ttcaggcgga cgttaccggc gacgaagcct ttcgtcgcat caatgaccgt cacgtcgcgc
     2761 ttgagcgcgt acatcatgcc agccgggttg aacgcgccga agcggttgta ggtgtacaac
     2821 tgatcgagcg cgaccacgtt ggccgtgacc gtgcggctgc actgcggaat cgcagccgtc
     2881 tgctggacgg cggcggcggt gatggctgcc gtcaacgctg cggcggtggt gatgggcgcg
     2941 gcagcggcgg cggtcgtggt tgtcgtcgtc gcggttgttg gtgcggcggc ggtcaccgtg
     3001 ccggaagcgg ggctgcccgc ttcgtttgcg gcgggcgcgc cggtcgcttc gggtgtggtc
     3061 gcagtcgcgt tcgacggcgt gactgacggg tcgctggcgt tcactttcgc cggtacggca
     3121 tcacccgtgc gggaaagttc ggcgttggta tcgccgggga gacgcgtggc gctcaaggaa
     3181 aaatgttgcc tggcggatag ctgctgcgcc aaggccggac tgctggcgag cacgatcaag
     3241 ccgaccagca gcaaacaact gctgacaagc ttcgaccatg cgccgcttct cctgtgcccc
     3301 ttcatatagt aattctcctt atcgggtaaa tggacggata atctggctcg ctctgctgcc
     3361 accagcctgt gaggccgagc cgtttcatgt tgcaggtagt tcaatttaat caacgcgcct
     3421 tccctccctc atttctttcg atccgaaggt tgtcgaagac gactgtatcg ttcggcgcgg
     3481 acgccgtggc cttctggccg accagcagga cttgcaagcc ggtaatgtcg aacggagtgg
     3541 ggtacgcggc ctgcgtggtc cacgttgcgc cgtccgggct ggtttcccaa ctgacggtat
     3601 cggctgcgcg gttgtggcgg aaacgccaga agcgttgctg cgtcgcgttg taaggaacag
     3661 acgtttgcgt cacgacgccg cccacagacc gttgcgtgat gagtgtgccg ctggtgctca
     3721 cgcctaacat caggtaattg ccgctcgtgg cacgcagcgc gtagaaagtg gcgaacccgt
     3781 tggtcgtcgg gacggcgacg gtttggacgg cgacgcgcgc gtcggtcaag tcaatggtcg
     3841 tggccgacac gtagccgtcg tagccgggca tggcggcggc gggcgtgatc tccagccgcc
     3901 cgttctgttc gacgatgttg gagccggacg tggcgagcgt gttccacttg gcggcgcgca
     3961 cgttgtcgtt gaagttgtcg ctcagaggga agagcgggcg atagcgttcg acgcggaagt
     4021 tgtcgaagat ggccgtgccg ggagcggcgt tggtcgtgcc ggttgcgccc gcgccgaatc
     4081 ccgtccgcac cgcgccgagc gagaaggccg gggtgactgt cttgcgcgtc gtccaggtca
     4141 cgccgtccgc gctcgtgtcg aagctgacgg tgttggcgtc gatgtcgtga cggaaacgcc
     4201 agtaacgaat ggtcgcgtcc cacgccaact gtgtgcggtc ggtgactccg ttgatcgtcg
     4261 agtcgcaggt gaacaaattc gcgccgacgg cgaagacgag gccgttgctg tcgtcgatat
     4321 aaagtttgaa gtaagtccag actgcgcccg cctggctcgc gggttgcact tcgacttgca
     4381 gtgtcttgtc tctgaagtcg aaaggtcccg ccatctccag accgttgtag ccggaggtgt
     4441 tcggctgcgg cgtcacttcg agctgcccgt tctgctcgct cacaaccgtc ggcgaagccg
     4501 ggtcgttcac tacccacatc tgcggatgga tggcgttgtc gttgaagttg tcggccatgg
     4561 cgacgtacgt ggtggggttc ggctcctgcc agaggttgtc gaagatgacc gtatcgctcg
     4621 gcgaggtcgc agtggctttc tggccggtga gcacgacctg caagttggta acgggaaacg
     4681 gcgtcgcgat gctgcgatgt atagtccacg tcgcgccgtc cgggctggtt tcccaattca
     4741 ccgtgtcgtc ggagcggttg tgacggaaac gccagaagcg ttgctgcgtc gcgttataag
     4801 gaagctgcga gttcgtcacg acgccgccca cagactgttg catcaagaac gtgccgctgc
     4861 tgacgacacc caacaataag tagttgccgc tcccgtcggc caagccgtag aaagttgcga
     4921 acccgttggt ggtcgggacc gagacggctt cgacgctcat gctcgtatcc gtcaggtcga
     4981 tggcggtaac tgacttgtag ccgtcgtagc cgggcatggc ggcggtgggt gtgacctcca
     5041 gacgcccgtt ctgctcggcg atgttggagc cgggcgtggc gagcacgccc catctggcgt
     5101 cgcgcacgtt gtcgttgaag ttatcgctga gggtgatgcc cgtgtaggcc agcaggttct
     5161 gcgagccttc gccggggttg atcacctgtc cgggcgtcgc gctgttcctg atagcctgac
     5221 cgaccgcatc gggagcatcg cccggacgcg tgctcaaata tctggccgca agaccgccga
     5281 cgatgggagc cgccgaagag gtgcccgagc ggatggaggt ggccgcatcg cccgtgacgt
     5341 gcgccgagac gatgtccacg cccggcgcaa acacgtcaac cgacgcgccg aagttcgagt
     5401 tgggcgaacg gttatcgttc tggtcggtcg cgcccaccgt gatcgcctcc ggcacgcgcg
     5461 caggcgtccg caaactcgcg tcgcccgtgt tgttgcccgc cgccactacc gtcgtgacgc
     5521 ccgccgcgat caaattcttg acggcaatat ccatcgtgcc gttgacggag gtggagacga
     5581 tggtgatgaa gctcaagttg gcgacggcag gtttgacgtg attacccgcc acccagtcaa
     5641 cgcctgagat gatgcgcgcg agcgtgccgc cattagcgca gtcgagcacg cgcaccgacc
     5701 acagcttcac gcccttcgcc acgccgtacg tgttgccgcc gagcagggct gcgacgtgtg
     5761 tgccgtgccc gttgcagtcc gctccggtgc cgcccgcgat gtagtcataa gcgaaggcag
     5821 cgcgtccgcc gaactcctga tgcgtgtagc gaatgccgcc atcgatgatg taggcgttga
     5881 cgcccgcgcc gttggcttca taggtgtagg tgttgctcgg agcgggggtg cgctggtcga
     5941 ggcggtcgag ttgccagggc gcgttcgtct gcgttgatgc tccctgaacc tctgcgtctt
     6001 cctctatgaa ctctactttc ggatggcggc tgagagcctc tgccgccgct tcgttcatct
     6061 ccacggagaa gccgcgcaag gcgttccggt aagtgaagcc gatggttccg ccgtgcaggc
     6121 gcaccagacg tgcggcttct tcgccgacct gttcgcgcct gacgctctcg ttgaagacca
     6181 cgatgtattg attcttgatt gcggcggcgt tccggcgcag tttgccctgc gaaacttcag
     6241 ggcgctgcgc ctgcgtgtgt gagggaacaa gtgcaatcgc gcacacaccg atgatcaaaa
     6301 gtaaggctgc caatctcttc attgttttcc tttgtattcc cgccttgatt cccatccctt
     6361 gccacagcgc gccggggtgg acgcgcccgc cgtagtctca acgagtcaag tccctgtgct
     6421 ttaagttgtt aatcgcaggc gcgtctataa cgcctgccaa tcgggataga agtcgtcagc
     6481 ggttgaagga tcgacgccgt tacccgtgtt gaaagtgatc ctgatgcggt tgccgccgtc
     6541 tgcatccatg atgtaaagct cgcccttgcc gtcgtccaaa ccgctctgga aaacaatgcg
     6601 gctgtcatcc gacgaccagg aagggttgta ttcgtaggcg gcgttgttcg agagattcac
     6661 acgcccgctg ccgtctgcgt tcatcacgaa aatctggtct gtgtgtgcag cattgccgcc
     6721 gtcgaagacg aacgaaagct gcgcgccgct accagaccag gcggggtagt aatcgttgtt
     6781 cgccccggtt gcaccgagat tattagtgat gttcacctga ttgctgccgt cggcgttcat
     6841 cgtgaagatt tcgatgttgt cgtaggcgac gggagagcca cggaaactgg gaaacgcgat
     6901 cttcgtgccg tccggcgacc acgtcggatc gatatccgcg ccggggctgt tggtgagacg
     6961 ccgctcgttc gtgccgtcca cgttgatgac gtagatttcc caatcgccgt cgcggctgga
     7021 acggaaggcg atctgcttgc cgtcgggcga ccaggcggat tccgcgttat gccccgtctg
     7081 gttgctggtg atgcgtctga cgttcgtgcc gtcggcgttc atcacgtaaa tctgatagta
     7141 gagatcgtcg ccgcgcccgc tcgtgaaagc gatctgcgtg ccgtcgggcg aatactccgg
     7201 ccagtagtcg tcgctgtaat cgtgtacggt gaggcgcgtc tcttcgctgc cgtccgggtt
     7261 gatggagtag atttcaaact ggccgtcccg atagctggag aaagcgatct tgccgttcgg
     7321 cctgtagagc aacaggttcg gcgagcctgc gccgatgttc ggtatcttgc cgggcgtcgc
     7381 gttgctcgtg atggctttgc tgacggaggc cggactcgct tgcgggttgc cgctcaggta
     7441 acgcgccgcc atccctgcga cgaagggcgc agccgaagag gtgccggaac gctgcgcgat
     7501 ggccgtatcg ctcgtgacgt gcgccgagaa gatgtccacg ccgggcgcga acacgtcaac
     7561 cgccgcgccg aagttcgagt tgggagattg attgtcgttc gggtcggtcg cgccgaccgt
     7621 gatcgcttcc ggcacgcgcg cgggcgagcg cagagaagcg tcgcccgtgt tgttgcccgc
     7681 cgcgacgacc gtggtgacgc ccgccgcgat catgttactg acggcaacgt caatggtgct
     7741 gttcgccacg atggtgatga agctcaagtt ggcgacggca ggcttaacgt ggttggctgt
     7801 gacccaatcc acacccgcga tgatgcgcgc gagcgtgccg ccattggcgc agtcgagcac
     7861 gcgcaccgac cacagcttca cgcccttcgc cacgccatac gtgtttccgc cgagcagggc
     7921 tgcgacgtgt gtgccgtgcc cgttgcagtc cgctccggtg ccgcccgcga tgtagtcata
     7981 agcgaaggcc gcacgtccgc cgaactcctg atgcgtgtag cgaatgccgc cgtcgatgat
     8041 gtaggcgttg acgcccgcgc cggagttagc gtagctgtac ttcgtgtcga gcgggagatg
     8101 gcgttggtct atgcggtcta aagcccacga cgggttggtc tggaccgatg cgccttcgac
     8161 gacggcatct tcctcgacat aagcgacgtg cgggctgcgg ctaagcgcga cggcctgtgc
     8221 ctcgttcatc tcgaccgaga aaccgcgcaa ggcagtttga tatgtgtagc cgagctggcc
     8281 gccgtgctcg cgcgccagtt gccgtgcgaa cgcggcgacg cgaccagccg gaacgctttc
     8341 gttgaaaact actatgtaat tattcgggat cgcgctttcg tttttacgga ggcgattctc
     8401 agagtcggat tcttgtccga gcgcgagttg gggaacaagc gcaatggctc cgaatatcac
     8461 tagcaatgcg agggtcttct tcatcgatgt ctccgtgttg tcttaaatca atggggctaa
     8521 caaacatggg ggcgtcgtta gattcagttc cttacatcgg ttgccagacg gggtcgtaat
     8581 cctcagccgc gttggtggtc agccttatgg ggttgctgcc gtcggcgttc atgatgaata
     8641 actcgtagtt gtagttgctg ccggcgtctc tcatgaagag aatccggtcg ccgcccggcg
     8701 accactgcgc tgcgacattc ccgtacgcct cctgcgaaga gaacgtcaac ctcagagggc
     8761 tggtgccgtc gggattgatt gtgtatagct cggtgaaacc gtaggggctg ccgttgtagc
     8821 gcgtgcttcc catgacgagc gttttactat cgggcgacca gtcaggctcg aaatcgcccg
     8881 cgccgtagta gggcgtgata ggcagtacgt tcgtcgccgc gctgctgccg tcggcgttca
     8941 ccacccacac gtcgtaatgc tcctcgtaac tgggggtgta aaagtagctc gcgaaagcga
     9001 tctttcgacc atccggcgac cagtccatgt agttctcatc gacgcgagcg gcggtcaggc
     9061 gaatttgatt ggtgccgtcg gcattcatca cgtaatgctt agtcaggccg tcgcggaagc
     9121 tgcgaaaagc tatcttgttt tcggtgggcg accacatcgg gtcgtactcc ggcgatacgc
     9181 tgcgcgtcac gttcacctga ttggtgccgt cggcattcat cacgaaaaca tcgaagttgc
     9241 cgtcgcgctt gctgatgaag gcgatgcgct ggctgtcagg cgaccacgtc ggggcttggt
     9301 cggcatacgt gctggtggta agcctcgtca gcccgctacc atcgctgttc atcacgtaaa
     9361 tatcgcccgc gcccgtgcga ttgctctgga aggctatttt ccgcccgtcc ggcgaccaga
     9421 cgacgggtag gtcttttgct gtgtgcagcg agatgttctt cggattgctc ccgtcggagt
     9481 tcatcagata gagttcccag ttgccatcac gcatactgga aaaagcgatc ttgctgttgg
     9541 gtttgaagag aaccagattc ggtgtgccaa ctcccgcatt caacactttg cccggcgtcg
     9601 cattcgacgt gatcgccaga ctcaccgccg ccgtcgtttc gttcggattc gcgctcagat
     9661 aacgcgccgc cgaacccgcc acaaaaggcg ccgcgaacga cgtgcccgaa cgcacgctcg
     9721 ttgaaacgtc gtcgaagtgc gacgcagaga tgatgtccac tcccggcgca tacacgtcta
     9781 ccgccgcccc gtagttagag aacggcgctt gattgtcgta ctggtctgtc gccgccaccg
     9841 tgatcgcttc cgtcacccgt gcgggcgaac gcaaactcgc ctcgcccgtg ttgttgcccg
     9901 ccgcgacgac ggtagtgacg cccgccgcga tcaggttccg cacggacagg tcaatcgtgt
     9961 cgctgcccac gacggtgatg aaactcaggt tcgccaccgc cggtttgacg tgattcgcgg
    10021 ccacccagtc aatgcccgcc aacacgcgcg ccgtcgtccc ctgattcaga cagttcagga
    10081 cgcgcaccgc ccacaacttc gcgtccttcg ccacgccgta ggtcttgccg ccgatgacgc
    10141 ccgccacgtg cgtgccgtgt ccgttgcagt ccacgccgtt gcgcccgtcg gccacgttgt
    10201 cgtaagcgaa gagcgcgcgc ccgccgaact cctgatgcgt caggcgaatg ccgccgtcga
    10261 tgacatagac gttggcgccc gtaccggcat tgagataggt gtaggtggtg tcgagcggaa
    10321 ggttgcgctg gtcaatgcgg tcgagtgccc aggacgctcc cgtctgcgtc gccgcgcctt
    10381 cgaccagcgc atcttcttcg acgtaagcga cgtgcggatt gtggctgagg gcgatggcct
    10441 gtgcctcgct catctcgacg gagaagccga gcacggcgtt ctggtaggtg aagccgagtt
    10501 ggccgcgatg ctcacgcgcc agttgggcgg caaacgcctc gacgcgagcc gccgggacgc
    10561 tatcgttgaa cacgacgatg taactatcgg aaatcgcgcc gtcgctttta cgcaggcgac
    10621 cctgggaatt gaggttttgt ccgagcgcga gttggggaac cagcgcgagt aagcagaacg
    10681 acagggagag aactttcagg aacttcatca aatatcccct ataaattttg tgtgggtggc
    10741 ggcgcaaagt taacttgttc aggtaagcca gcgagacagc aaacggcttg tctccgcgac
    10801 ccaaacttcc gcccgtaaat ccggcgtaga agctaacccg ctcatccggt aatagttgtt
    10861 gtgaagcgtt ggtaacggtg cgagccggaa acgactttat ctagccttct acttcgacaa
    10921 aatcgttatg agttcgctgt tagggggttt gtctccgact tgcggctcta tctagtgcaa
    10981 gcagcgttcc gcaaggttgt agagccttaa atatagacag gacatggttt agcggaaaca
    11041 aagggtggca gggcaaaagg cgtgcgtgtt tcactatgaa actcgcgccc ggcacgagtt
    11101 tcatagtgaa acaaaatata aatacaagca tttcgcaaca aagcgcgtcc gccgccgcta
    11161 aatgttgcag acgcattctg gagactggcg ggcagttgcg acttccgcga gactcaaatt
    11221 cagccgctca cttatctctt tgataatgct ggatttacct gtaggagttg aacttagcgg
    11281 gcgcgggcgg gaaattttgc ggcggagact ggcggccatg agtagtcttt ccggctccgg
    11341 ctcgacgccg cgtgttttat tttgtgtctg cgccgggtgg gcgtcgaagt tttgggcacg
    11401 cgccggggta ggcttgtgcg cggcgggatt agttgttgat cagtttgaat cgcttcaatt
    11461 tgcggtagag ggtcggcctg tgcatctgga gtatctgcgc ggtttttttg acgttgcctc
    11521 cggcgcgttc gagcgtctgg gcgatggctt cgcgctcgat ctcttcgagc gtgagatgcg
    11581 gcgggatgag gcactttgat tgacccgccc cggcggagtt caggtgttcc ggcaggctct
    11641 cgatggtgag ttgttcctcc tgacagaaaa ggactgcgcg ctcgacgacg ctctcaagtt
    11701 cgcgtatgtt gcccggccag tcgtagcgca tcaggagcga gagcgcgggt tgcgcgatgc
    11761 cgcgaatcgg cttgtgatac ttctccgcgt agcgttgcag aaaatggtct gcgagcggga
    11821 gcaggtcgtc gggtcgctcg cgcagagggg ggacagtgat cttgacggtg ctgatgcgga
    11881 agtagaggtc ttcgcgcagg agtccttcgc gcgtggcctg cgccgtgttg cgattggtgg
    11941 cggaaaccag acggaaatca accgggatct cctgctcgtc gccgagacgg cgcagtttcc
    12001 gttcttgcag cacgcgcaac agtttggctt gcaggtgtgc gggcatctcc gcgatctcgt
    12061 ccaggaggag cgtgccggaa gcggcggctt cgatcaagcc gcgcttgtcg cggtcggctc
    12121 cggtaaacgc acccttcttg tagccgaaga gttccgactc gatgagatcg ggcgggaatg
    12181 ccgcgcaatt gatgcgtatg aaagggcggc tcgcacgttt gctctggagg tgaaaggcgg
    12241 tagcgatcag ttctttgccc gtgcccgatt cgccttcgat gaggacggag gcttccgagg
    12301 gcgcagtggc gcgaatagtc tcgtaaatct gccgcatctg cgggctgcgt ccgatgatgc
    12361 cgtaggcttc cttttcctct ccgagcgtac gcttgagcac gcggttctcc tgtttgatcc
    12421 ctgcaaactg gagcgcgcgt tcggtaagca tgatgatctc gtcgggcgtg aaaggctttt
    12481 cgacgtagta gtacgcg
//
LOCUS       DASWNS010000666        12507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3420
            k141_389139, whole genome shotgun sequence.
ACCESSION   DASWNS010000666 DASWNS010000000
VERSION     DASWNS010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959239
            Sequence Read Archive: SRR10954988
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 09:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,619
            CDSs (total)                      :: 4,552
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 67
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 4 of 24
            Pseudo Genes (incomplete)         :: 18 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389139"
                     /isolate="SMAG_U3420"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VGB76_23015"
     CDS             <1..658
                     /locus_tag="VGB76_23015"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0101816.1"
                     /translation="GLNFVQNVPPAARAAYESALKLLRDARTPDALAALQEAVKIFPD
                     YFDARFLLAAEYSKGGKLTEAITELNEAQRINSKDDRVWHAFGGVLMQQRKFAVAARV
                     FAEAARLSPSDPQYHLLQATALIEQAASIEPAKADERDYAFAEAEKSLARADQLSARK
                     LAAVHLQRARLYEKRGDRARAADELERYLRQSPELKNAAAIRQAIKTLRTSASDKKTP
                     "
     gene            820..2235
                     /locus_tag="VGB76_23020"
     CDS             820..2235
                     /locus_tag="VGB76_23020"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY0101817.1"
                     /translation="MADDREIKDLERTLEQIWEVARRFGLDPFPTRFEIVPATVMYEV
                     GSYALPGRYSHWTFGKAYHRMKTMYDFGLSKIYEVVINTNPSYGFLLETNSPIQNKLV
                     MAHVLGHVDFFKNNVYFSKTNRRMVESVSSHAGRMSAYEFKYGRKTVEEFLDAVLSIE
                     EHIDPNFFIKQDRPVQTPEQQRAGVPKEGRYDDLWKLGEPKGAPSVPAPELPRGERLP
                     EKDLLYYIMRNSPSLEDWQRDAMGMIHEEMEYFIPQMQTKVMNEGWASFHHARIMREL
                     DLSDRELLEFAELHAGVVSPQKGQLNPYYLGYKIFEDIERRWNNPTAEERERFGRKGG
                     EGREKIFEVRELDNDVSFLRNYLTEELCEELDLFVYELVEEEEWTVTEKRWERVRDQL
                     VSNMTNFGHPYIVVADGDYNRNRELYLKHQYESAELDASYARKVLEYVYRLWGRPVHL
                     ETTADGEPVVMRYNGEEHAED"
     gene            2418..2933
                     /locus_tag="VGB76_23025"
     CDS             2418..2933
                     /locus_tag="VGB76_23025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101818.1"
                     /translation="MRNNYLPATAKNFRTMLFILLIALTGIQTPGVDAFAQRQTAPTA
                     LSAELKSSTEAKRVLANIGSRDFKRIRDARLRAATSRAVDALKAVARNTSAAREAALV
                     VKFNRTVKDLKSLAKPSTEFQECDNQYNLCIEICKEIGSDCDLCGFGQYGCYLNKLAI
                     EMTKDPLDPTP"
     gene            2978..3442
                     /locus_tag="VGB76_23030"
     CDS             2978..3442
                     /locus_tag="VGB76_23030"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0101819.1"
                     /translation="MSRRSAFVKESLIRAEPARVFAFHELPDALARLTPPWESSRVVQ
                     AAKISETGSEAIVETRILGIIPARWVARHTIYDPPRLFEDVQVKGPFRFWRHRHLVEP
                     HAEGSILRDEIEYEPPFGLVGQLVKPFLITPRLTKLFDYRHAATRAWCEKEK"
     gene            complement(3545..4711)
                     /locus_tag="VGB76_23035"
     CDS             complement(3545..4711)
                     /locus_tag="VGB76_23035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101820.1"
                     /translation="MSNTARVPTAGNPEAQELFMSATTSNTTTSAATLIAPVGAQARS
                     LSVRWIISARDDLVWFIGSVVSSYALFALYVGGIVPLVPMVAAWAILIDAPHVFGTFS
                     RTYFDREERRARARLLWGSLAFFAVGPLLVLAGLGLVFFFLAALWAYYHLVKQHYGFM
                     VLYKKKNADLAPVDNALDRLFILLALSYPFVAFVARDPEALARVPAKLSGGIAYLETA
                     LLAATVCVALLWTLRQAQRAVQRQPLDVPKYLLLAACVPMHWAVLLTPMPHKPIAIVA
                     ILTVYHNLQYHRLIWFHNRKYAGGGEGRARYGAAELISRRLIYYLAFGILFGLWYQVP
                     RQYISHTSGRAALLTQLVAAFFWGYAFIHYYLDSKIWRVRRDPSVGHALNLSDK"
     gene            4820..5518
                     /locus_tag="VGB76_23040"
     CDS             4820..5518
                     /locus_tag="VGB76_23040"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HEY0101821.1"
                     /translation="MPTNDEALHALLAKLFQAHPWHGVVPGGKDAPSIVNAYIEIVPT
                     DAVKYELDKPSGHLRVDRPQRFSSMCPTPYGFIPQTYCGQTVAALCEARVGRTGIRGD
                     GDPLDICVLTERPAAHGDFFVRALPIGGLRMIDGNEADDKIIAVLESDVTYGHLRDIA
                     DAPTGLIDRLQHYFLSYKQLPQEAPRHVEIAEVYRREDAWEVIRRSVEDYRERFGAPE
                     TRIAELRRLLIDKG"
     gene            complement(5665..5901)
                     /locus_tag="VGB76_23045"
     CDS             complement(5665..5901)
                     /locus_tag="VGB76_23045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101822.1"
                     /translation="MKHFVNNGYGWTCRHCRREETAARVPETNGARARFFSEGEAEER
                     EPQLSVSSLARWRDESRRELVCPKCGVIEKTEQR"
     gene            complement(5990..7135)
                     /locus_tag="VGB76_23050"
     CDS             complement(5990..7135)
                     /locus_tag="VGB76_23050"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0101823.1"
                     /translation="MYRISARQILLIAFTSAFFAASVVFFFERYGSRLLEPSGSASLT
                     ENAPAGITNPTLATDEQNNIEVYKAISPGVVFITSTSFSNDFWDAGERSGTGSGFVID
                     AQGHILTNYHVIEGAQKLSVSFGDKSYPATKVGDDPDTDLAVIKVNAPRDVLKVIPFG
                     DSDNLVVGQKVLAIGNPFGFARTLTTGVISGLQRPIRARNNRQIEGAIQTDASINPGN
                     SGGPLLDSSGRVIGINSQILSPAGGSVGVGFAVPANIAKRIVPQLIKNGAVVRPKLGI
                     VPFNVANLQMRLPIAEGVGIYQVYPNTSAAAAGLRGLTQTPDGDVELGDIITSIDGEA
                     VNNSDDLYRILDKHQFNDVVQVEVFRDNRRTKVPVRLLPDRRGVLRR"
     gene            complement(7188..7325)
                     /locus_tag="VGB76_23055"
     CDS             complement(7188..7325)
                     /locus_tag="VGB76_23055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101824.1"
                     /translation="MRQLTRVIQTFTRLFAYQLLTPTGVDASFRVLAVRLDNGDSAAR
                     I"
     gene            complement(7428..7796)
                     /locus_tag="VGB76_23060"
     CDS             complement(7428..7796)
                     /locus_tag="VGB76_23060"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEY0101825.1"
                     /translation="MSALTKCLLLFLLNWLDAQLTIIWVRNGLATEGNGLMAYLLDAG
                     NAPFLITKLCVGAGVAFVLYRWSHLALARRGLSLVLGLYFALMFVHAATGISALGHPQ
                     ADAAIAYLVRLPGALLTALL"
     gene            complement(8047..8295)
                     /locus_tag="VGB76_23065"
     CDS             complement(8047..8295)
                     /locus_tag="VGB76_23065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0101826.1"
                     /translation="MEERNEHAVDDRGTSETGGREILKRLRDEGFEADDEKLAVALGR
                     PIEEVQGWMAGGGSDEPVDDDIIMKARGIAKERGIALE"
     gene            complement(8316..8555)
                     /locus_tag="VGB76_23070"
     CDS             complement(8316..8555)
                     /locus_tag="VGB76_23070"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY0101827.1"
                     /translation="MKSEFHIKGMHCESCAVDIKETLETTAGVFRADVTYGGKTANVE
                     YDDNVVQEKTLIKKIQDLGYDATIGDDTQSSEATA"
     gene            8697..9590
                     /gene="prmC"
                     /locus_tag="VGB76_23075"
     CDS             8697..9590
                     /gene="prmC"
                     /locus_tag="VGB76_23075"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY0101828.1"
                     /translation="MSEQPHSNRTKIGEALHEGIARLRAAAIADARPDARLLLSHTLM
                     RDHAYLVAHSEEEIEPPALALFRALLARRAAGEPVQYITGRQEFYGLDFEVNSAVLIP
                     RPETELLVETALELLRETSAPLLCDVGTGSGCIPVTLLHERRDARAFGLDISTDALAV
                     AARNAARHAVSERLALRVSDCFAALDAPHASFTLITSNPPYIPARDIPQLQREVRDHE
                     PRIALTPPGDEDGDGLSIIRRLIGEAAPYLIPGGHLLLEIGHDQHEAVARLVAPAVWT
                     LLDTRRDLQGIPRTVVLRRRA"
     gene            9744..11060
                     /gene="murA"
                     /locus_tag="VGB76_23080"
     CDS             9744..11060
                     /gene="murA"
                     /locus_tag="VGB76_23080"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY0101829.1"
                     /translation="MDKFRITGGQALRGRINVSGAKNSALPCMAAALLTGESVTLHNV
                     PYVRDIITQRRLLEDLGAHVLTPELRTHKITAAHIELFEAPYDLVKTMRASVLVLGPL
                     LARFGKAKVSLPGGCAIGSRPIDLHLESLKALGAEVSLEAGYVVARAPRGGRLRGATV
                     NFDKVTVTGTENLLMAATLASGTTTLNHAACEPEVADLAELLNRMGARVRGAGTPSIT
                     IEGVETLGAAEHTIIPDRIETGTFLVAAAITDGELELKDCTPEHSATVIAKLREAGVE
                     IEEVNPSTLHVRRAPGGLKASDITTEPHPRFPTDMQAQYMTLMTQSQGRASITETIFE
                     NRFMHASEMQRMGARIAIEGRTAIVEGATPLAGARVQASDLRASASLVLAGLVAEGET
                     IIERVYHIDRGYEKIEDKLRGVGARIERLRDSVTASLPPIAEVGVL"
     gene            11182..11532
                     /locus_tag="VGB76_23085"
     CDS             11182..11532
                     /locus_tag="VGB76_23085"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY0101830.1"
                     /translation="MSQQSCIFCQVVAGEISADIVYRDDRAVAFRDINPQAPVHLLVI
                     PHEHLDSLDDAAGRDEATLGHLLRIAARVANEQGLSESGYRTVINTGAGAGQSVFHLH
                     LHVVGGRPLHWPPG"
     gene            11547..11972
                     /locus_tag="VGB76_23090"
     CDS             11547..11972
                     /locus_tag="VGB76_23090"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY0101831.1"
                     /translation="MYEESEDPKAVVSRWFEEVWNKGREEAIDELFAEDGVAHGLADE
                     TGEPLRGASGFKPFFRNFRSAFPDIEVVVEDAIAEGDKVAARCRVRATHAGDSLGFAA
                     TQRAIEFTGMTFLRVRDGKIVEAWNNFDFLGLRQQLGAL"
     gene            complement(12064..>12507)
                     /locus_tag="VGB76_23095"
     CDS             complement(12064..>12507)
                     /locus_tag="VGB76_23095"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0101832.1"
                     /translation="VFGIPLALVLSIVTFLTGFFPLLGEWAIYVPVSVYLLVFRNDPV
                     GASVYHVIGIMMTVGSSLLLRPRVAASGAGRFNFYWMFLALIAGVYTFGIPGIVLGPA
                     IIGFVKAISDTLLGNVKYETSLLKEEKVQQAGQQQEERLKQRAAS"
BASE COUNT         2590 a         3843 c         3765 g         2309 t
ORIGIN      
        1 cggtctgaac ttcgtccaga acgtgccgcc cgcagcgcgc gccgcctacg agtcggctct
       61 caaactgctc agagacgcgc gcacgcccga tgcgctcgcc gcgctgcaag aagccgtcaa
      121 aattttcccg gactacttcg acgcgcgctt ccttctcgcc gccgagtaca gcaaaggcgg
      181 caaactcacc gaagcgatca cggagttgaa cgaggcgcag cgcatcaact cgaaggatga
      241 tcgcgtgtgg cacgcgttcg gcggagtgtt gatgcagcaa cgcaaattcg ccgtggcggc
      301 gcgcgtcttt gccgaagcgg cgcggctgag tccgagcgat ccgcagtatc acttgctaca
      361 ggccacggcc ttgatcgaac aggccgcgtc tatcgagcca gccaaagccg acgagcgtga
      421 ctacgctttc gccgaagccg agaagtcgct cgcacgcgcc gaccaactga gcgcacgcaa
      481 actcgccgcc gtccacctcc aacgcgcacg cctctacgag aagcgcggcg accgcgcacg
      541 cgccgccgac gaacttgagc gctacctgcg tcaatcgccc gaactcaaaa acgccgccgc
      601 catccgtcaa gcgatcaaaa ccctgcgcac gtccgcgagc gacaagaaga caccttagga
      661 atcaaggatg aacaagaatg aacgcggaag gatgaacgat gagtgaaagt atcaattcgc
      721 cgtccggctt tcttattcat cgttccgcgt tcatcgtttc gcgttcatcc ttcgctgtcg
      781 cgccttgccc ctgcgccgct tggagaggaa aatacgtgta tggcagacga ccgcgaaatt
      841 aaagacctcg aacgcacgct cgaacagatc tgggaagtgg cgcgcaggtt cggcctcgat
      901 ccgttcccca cgcgcttcga gatcgtgccc gccacggtga tgtatgaagt cggctcttac
      961 gccctgcccg gtcgctattc gcactggacg ttcggcaaag cctatcatcg catgaagacg
     1021 atgtacgact tcggcctctc gaagatttac gaggtggtca tcaacaccaa cccgtcctac
     1081 ggcttcctgc tcgaaaccaa cagtcccatc cagaacaaac tcgtgatggc gcacgtgctc
     1141 ggccacgtgg acttcttcaa aaacaacgtc tacttctcga agaccaatcg ccgcatggtc
     1201 gaaagcgtct cttcgcacgc ggggcgcatg agcgcctacg aattcaagta cgggcgcaag
     1261 acggtcgaag agtttttaga cgccgtgctc tccattgaag aacacatcga cccgaacttc
     1321 ttcatcaaac aggatcgccc cgtgcagacc ccggaacagc agcgcgcagg agttcccaaa
     1381 gaaggccgct acgacgatct ctggaaactc ggcgagccga aaggcgcgcc ctccgtgccc
     1441 gcgcccgaac tcccgcgcgg cgagcgtctc cccgaaaaag acctcctcta ctacatcatg
     1501 cgcaactcgc cctcgctcga agactggcag cgcgatgcga tgggcatgat tcatgaagag
     1561 atggaatact tcatcccgca gatgcagacg aaggtgatga acgaaggctg ggcgtcgttc
     1621 caccatgcgc ggatcatgcg cgaactcgat ctgtcggatc gtgaactgct ggagtttgcc
     1681 gaactccacg cgggcgtcgt ctccccgcag aaagggcaat tgaaccctta ctatctcggc
     1741 tacaaaatct tcgaagacat cgagcggcgt tggaacaacc cgaccgcaga ggagcgcgag
     1801 cgtttcgggc gcaaaggcgg cgaggggcgc gagaagattt tcgaagtgcg cgaactcgac
     1861 aacgatgtct ctttcctgcg caactacctg accgaagagt tgtgcgagga gttggacttg
     1921 ttcgtctatg aactcgtgga agaagaagaa tggacggtga cggagaagcg ttgggagcgc
     1981 gtgcgcgatc aactcgtctc gaacatgacc aacttcggtc atccctacat cgtcgtcgcc
     2041 gacggcgact acaaccgcaa ccgcgaactc tacctcaagc accagtacga aagcgcggaa
     2101 ctcgacgcct cctacgcgcg caaagtcctc gaatacgtct atcgcctctg gggtcgcccc
     2161 gtccatctcg aaaccaccgc cgacggcgag ccggtcgtca tgcgctacaa cggcgaagag
     2221 cacgccgaag actaacccgg cgtaaataat caccttgagt taatcgtgcg acggggagac
     2281 gaaaaaaatt gtcgtcagga tgatagtttt cgcttcgatt ttccgcctta agaattaaga
     2341 caaatcgaat ggccgcgctt cctccggtca ttaacattat tgccagctta attctcaagt
     2401 gagaaggaga atggaacatg cgaaacaact acctcccggc gaccgcgaag aatttcagaa
     2461 cgatgctgtt catcctgtta attgcgctta ccggcataca gacgcccggc gtggatgcct
     2521 tcgcgcagag gcagaccgcg ccgaccgcac tgagcgcaga attaaagagc tccaccgagg
     2581 ccaagcgcgt gctcgccaac atcggctcaa gagacttcaa acgaatcagg gatgcaagat
     2641 tgcgtgcggc gacgagtcgc gctgtagatg ctttgaaggc ggttgcgaga aacacctcgg
     2701 cggcgcgcga agcggctctg gtcgttaaat tcaaccgcac cgtcaaggat ttaaagtcgc
     2761 tggcaaaacc ctccacggag tttcaggagt gcgacaatca gtacaatctc tgcatcgaga
     2821 tatgcaagga gatcggcagc gactgcgatc tctgcggctt cggacagtac ggatgctacc
     2881 tgaataaatt ggctatagag atgacgaaag acccgcttga cccgacgccg taactgattc
     2941 gtcgccgagg caagattgaa gtcggagagg agagaggatg agtcgccgca gcgcgttcgt
     3001 caaagagagc ctcatccgcg ccgagcctgc gcgcgtcttc gcgtttcacg aactgccgga
     3061 cgcgctcgcg cgcctcacgc cgccgtggga atcttcacgc gtggttcaag cggcaaagat
     3121 ttccgaaacg ggttcggagg ccatcgtcga gacgcgcatc ctcggcatca tccccgcgcg
     3181 ctgggttgcg cgccacacga tttacgatcc gccgcgcctg tttgaagacg tgcaggtcaa
     3241 aggacctttc cgtttctggc gtcatcgtca tctcgtcgag ccgcacgccg agggcagcat
     3301 cctgcgcgac gagatcgaat acgagccgcc gttcggcctt gtcggtcaac tcgtcaaacc
     3361 attcctcatc accccgcgac tcacgaaact cttcgactat cgccacgccg ccacccgcgc
     3421 ctggtgtgag aaagagaagt gatgaaagaa aagtgatgag taatgagtga tgagtgatga
     3481 gctaaaacaa cagccttgtc tttcactcat tactcattac tcatcactca tcacttcttc
     3541 cttgtcactt gtcactgagg ttgagtgcgt ggccgaccga ggggtcgcga cggacgcgcc
     3601 agattttcga gtcgagatag tagtggatga aagcgtagcc ccagaagaag gctgcgacga
     3661 gttgtgtgag aagtgccgcg cgcccgctcg tgtggctgat gtactgtcgc ggcacctgat
     3721 accagaggcc gaagaggatg ccgaaggcca gatagtagat gagtctgcgg ctgatgagtt
     3781 ctgccgcgcc gtagcgcgcg cgcccttcgc cgcccccggc gtacttgcga ttgtgaaacc
     3841 agatgaggcg atgatattgc aggttgtgat agacggtgag aatggcgacg atggcgatgg
     3901 gcttgtgggg catcggcgtt aagagcaccg cccagtgcat cggcacgcag gcggcgagca
     3961 gcaggtactt cggcacgtcg agcggttgtc tctgcacggc gcgctgcgcc tgccggagcg
     4021 tccacagaag cgcgacgcag acggtcgccg cgagcaacgc ggtttcaaga taagcgatgc
     4081 cgcccgacag tttcgcgggg acgcgcgcca acgcttccgg gtcgcgcgcc acgaaggcga
     4141 cgaagggata agagagcgcg aggaggataa agagccggtc gagcgcgttg tcaacgggcg
     4201 cgaggtcggc gttcttcttt ttgtagagca ccatgaaacc gtagtgctgc ttgacgagat
     4261 ggtaataagc ccagagcgcg gcgaggaaga agaagacgag gccgagtccg gccaagacca
     4321 gcagggggcc gacggcgaag aaggcgagcg acccccagag caggcgcgca cgcgcacgcc
     4381 gctcctcgcg gtcgaagtag gtgcgcgaga acgtgccgaa gacgtgcggc gcgtcgatca
     4441 atatcgccca tgccgccacc atcggcacga gcggcacgat gccgccgacg tagagcgcga
     4501 agagcgcgta gctcgacacg accgacccga tgaaccacac gagatcgtcg cgcgcgctga
     4561 tgatccagcg cacggacaga gagcgcgcct gcgccccgac aggtgcgata agcgtggccg
     4621 cgctggtcgt ggtgttggag gtggtggccg acatgaaaag ttcctgtgct tcggggttgc
     4681 ccgctgttgg cacgcgcgct gtgttggaca cgcgacgcgc gctcgcttca taatctgccg
     4741 gaagattcag acgcgcgcgg agacgaattt tagcataacg gcgaggccgc gcgcggcctt
     4801 gtaagttgag aggaaacgga tgccgactaa cgacgaagcc ctgcacgctc tgctggcaaa
     4861 actttttcag gcgcacccct ggcacggcgt cgtgcccggc ggcaaggacg cgccctcaat
     4921 cgtcaacgca tacatcgaga ttgtccccac cgatgcggtc aagtatgaac tcgacaagcc
     4981 ttcgggacac ctgcgcgtcg accgtccgca gcgtttctcc tcgatgtgcc cgacgcctta
     5041 cggcttcatc ccgcaaacct actgcggcca gacggtggct gcgctctgcg aagcgcgtgt
     5101 cgggcggacg gggattcgcg gcgacggcga cccgctcgac atctgcgtgc tcaccgagcg
     5161 acccgccgcg cacggcgact tcttcgtccg cgccctcccc atcggcggcc tgcgcatgat
     5221 cgacggcaac gaggccgacg acaagatcat cgccgtcctc gaatccgatg tcacttacgg
     5281 ccacctgcgc gacatcgccg acgccccaac cggactcata gatcgtctcc aacattactt
     5341 cctgagttac aagcaactgc cgcaggaagc cccgcgccac gtggagatcg cggaagtcta
     5401 tagacgcgaa gacgcctggg aagtgatccg ccggagcgta gaggattacc gcgagcgttt
     5461 cggcgcgccc gaaacgcgca tcgcggagtt gcggcgtttg ttaatagata agggatgact
     5521 tttagagccg gtgagaagag gataaaaacc ttcaccggcg caagcgcaga gagggcgcag
     5581 agaaaagatt cactctttct ctgcgccctc tctttgtttg aggggatgag ttgcgaaagg
     5641 aacaagcaac ttgaaaggaa cggcttaacg ctgctccgtc ttctctatca cgccgcactt
     5701 cggacagacc aactcccggc gtgattcgtc gcgccagcgc gcgagcgacg agacggagag
     5761 ttgcggttcg cgttcttccg cttcgccttc gctgaaaaaa cgagcgcgtg cgccgttcgt
     5821 ctccggcaca cgcgctgctg tctcttcccg tcggcaatgg cggcacgtcc aaccgtaacc
     5881 gttgttgacg aaatgtttca taaacccgga ggccggaggt cggagtcagt tgaagacaag
     5941 aaccgatctc tactgatctc taacctccga cctctgacct ctactccggc tatcttctca
     6001 aaacgccgcg ccggtcgggc aagaggcgca cgggaacttt ggtgcggcgg ttatcgcgga
     6061 acacctccac ctgcacgaca tcgttgaatt gatgcttgtc gaggatgcgg tagaggtcgt
     6121 cgctgttgtt cacggcctcg ccgtcaatgc tggtgatgat atcgccgagt tccacgtcgc
     6181 cgtcgggcgt ctgcgtcagg ccgcgcaagc ccgccgccgc cgccgaggtg ttcgggtaga
     6241 cctgatagat gccgacgcct tccgcgatgg gcaggcgcat ctgcaagttg gcgacgttga
     6301 acggaacgat gccgagtttc ggacgcacga ccgcgccgtt cttgatcagt tgcggcacga
     6361 tgcgcttggc gatgttggcc gggacggcga agccgacgcc gaccgagccg cccgcaggcg
     6421 acaggatttg cgagttgatg ccgatcacgc gcccgctcga atcgagcagc gggccgccgg
     6481 agttgccggg gttaatcgag gcatcggtct ggatcgcgcc ttcaatctgg cggttgttgc
     6541 gtgcgcggat cgggcgttgc agaccgctga tcacgcccgt cgtcaaagtg cgcgcgaagc
     6601 cgaacgggtt gccgatggct aacactttct ggccgaccac cagattgtcc gaatcgccga
     6661 aggggatcac cttcaacacg tcgcgcggcg cgttaacttt gatcacggcc aagtccgtgt
     6721 cggggtcgtc gcccaccttc gtcgccgggt aagacttgtc gccgaaactg acgctcaatt
     6781 tctgcgcgcc ttcgatgacg tgatagttgg tgaggatgtg tccctgcgcg tcgatgacga
     6841 atccggagcc tgtgccgctg cgctcgccgg cgtcccagaa gtcgttgctg aaagaggtcg
     6901 aagtgatgaa gacgacgccc ggcgagatgg ctttatagac ctcgatgtta ttctgctcgt
     6961 cggtggcgag ggtcgggttg gtgatgcctg cgggcgcgtt ctccgtcagc gacgcgctgc
     7021 cgctcggttc gagcaggcgg ctgccgtagc gttcgaaaaa gaagacgacg ctagccgcga
     7081 agaaggccga cgtaaacgcg atcagcaaaa tttggcgtgc ggaaattcga tacataatct
     7141 gtttcctttg aaatgacaaa aaacataaga gacgcgtgtg ccttatttca gatgcgtgcg
     7201 gcggagtcgc cgttgtcgag tcgcacggcc agcacccgaa aagacgcgtc aacgcccgtc
     7261 ggcgttaaaa gctgatacgc gaaaaggcgc gtgaaggttt gaataactcg tgtgagttgg
     7321 cgcattcaaa ataatgcatt gaggaggcgc gttagaataa cttccgggcg ggtaaaaaag
     7381 ggcgggagtt gagacggggc gagggggagt tacgaatttt cggaaactca gaggagcgcg
     7441 gtgagcagtg cgccgggcag gcggacgaga taggcgatgg cggcatcggc ctgcgggtgg
     7501 ccgagggcgg agattccggt ggcggcgtgg acgaacatca gcgcgaagta gaggccgagc
     7561 acgaggctca ggccgcgacg cgcgagcgcg agatgcgacc agcgatagag cacgaacgcc
     7621 acgcccgcgc ccacgcagag cttggtgatg agaaacgggg cgttgcccgc gtcgagcagg
     7681 tacgccatca gcccgttgcc ttccgtcgcc agtccgttgc gcacccagat gatcgtgagt
     7741 tgtgcgtcga gccagttcag cagaaacaac agcaggcatt tcgtcagcgc gctcatcggt
     7801 tacgactctc cttgccctat ggactaactt tgtttaaccc tgccccttaa cgattgaacc
     7861 cccagcttat tgggctgggg gttcgctcag gctacgggag aaacgaattc gcttcgtttg
     7921 tatcaactag aaacaacgaa tgctcgacgc gttcctaaaa ctggtgaatc gtaaatcgta
     7981 aatcgtgaat agaaagaact ggccttcttc tgtttacgat ttacgattca cgatttacat
     8041 tcttccttat tccaacgcga tgccgcgctc tttggcgatg ccgcgcgctt tcatgatgat
     8101 gtcgtcatcg acgggttcgt cgctgccccc gcccgccatc cagccctgca cctcttcgat
     8161 gggacgaccg agcgcgacgg ccaacttttc gtcgtcggct tcaaatcctt cgtcacgcag
     8221 ccgcttcaaa atttcgcggc ctcccgtttc gcttgtccca cggtcgtcaa ccgcatgctc
     8281 gtttctctct tccatttagg ttcccctttt ttcgtttatg ctgtggcctc ggacgattgc
     8341 gtgtcatcgc cgatggtcgc gtcgtaaccc aagtcctgta tcttcttgat gagcgtcttt
     8401 tcctgcacca cgttatcgtc gtattcgacg ttggcggtct tgccgccgta ggtcacgtcg
     8461 gcgcgaaaga ccccggcagt cgtctccagc gtctccttga tgtccacggc gcacgattcg
     8521 cagtgcatgc ccttgatatg aaactccgac ttcatctgag tgcctcccct cgtgacgcgt
     8581 gcgctcgccc ccggcgcgac tcttcatgca ctgaggcgca agccgtgcga gcgaactctc
     8641 cccgcgttag attgtcatct tgcctgatgt gctgcgtatt atacagcaga acgtagatga
     8701 gcgagcaacc gcacagcaac cgcacgaaga ttggagaggc tttgcacgaa ggcatcgcac
     8761 gccttcgggc ggcagccatc gccgacgcgc gacccgacgc ccgcctcctg ctctcgcaca
     8821 cgctcatgcg cgaccacgcc tatctcgtcg cgcattccga agaagagatc gagccgcccg
     8881 cactcgcgct ctttcgcgct ctacttgcgc gccgcgcggc gggcgaacct gtgcagtaca
     8941 tcaccgggcg gcaggagttt tacggcctcg actttgaagt caattccgcc gtgctcatcc
     9001 cgcgccccga aaccgagttg ctcgtcgaaa ccgcgctcga actcctgcgc gagacgagcg
     9061 cgccgctgct ctgtgatgtc ggcacgggat cgggctgcat ccccgtcacg ctcctacacg
     9121 aacgcaggga cgcgcgcgcc ttcgggctgg acatctcgac ggacgcgctc gccgtcgccg
     9181 cgcgcaacgc cgcgcgccac gccgtcagcg aacggctcgc gctacgcgtc tccgattgct
     9241 tcgccgcgct cgatgcgccg cacgcttctt tcacgctcat cacttcgaat ccgccttaca
     9301 ttcccgcgcg agatattccg caactgcaac gcgaagtgcg cgaccacgaa ccgcgcatcg
     9361 ccctcacgcc ccccggcgac gaagacggcg acggcctctc catcatccgc cgtctcatcg
     9421 gagaggccgc gccctacctc atccccggcg ggcacctgct tctggagatc ggccacgacc
     9481 agcacgaggc cgtcgcgcgc ctcgtcgctc ccgccgtgtg gacgttgctc gacacgcgcc
     9541 gcgacctgca aggcattccg cgcacagtcg tcctgcgccg ccgcgcgtga gactgaaact
     9601 tccagaggca tctagtcgcc gtccggtcgt cgtgttaaaa tccgtcaact ttaaagcgca
     9661 cacggttcag tcagcatccg acgggtcaag atcacggagc aaactttgct ggcggcggat
     9721 agctaacagc taacaacttc tttatggata aatttcgcat caccggcggg caagccttgc
     9781 gcggtcgtat caacgtgagc ggggcgaaga attccgcgct gccctgtatg gccgccgccc
     9841 tcctgacggg cgagagcgtg acgctgcaca acgtccctta cgtgcgcgac atcatcaccc
     9901 agcgacgcct gctcgaagac ctcggcgcac acgtcctgac gcccgaactg cgcacgcaca
     9961 agatcaccgc cgcgcacatc gaactcttcg aagcgcctta cgatctcgtc aagacgatgc
    10021 gcgcttccgt gctcgtcttg ggtcccctgc tcgcgcgttt cggcaaggcg aaagtaagtc
    10081 tgccgggcgg ttgcgccatc ggctcgcgcc cgattgatct gcaccttgag agtttgaaag
    10141 cgctcggcgc ggaagtgtcg ctcgaagcgg gctacgtggt ggcgcgtgcg ccgcgcggcg
    10201 ggcgtctgcg cggcgcaacg gtcaacttcg acaaggtgac cgtgacgggc acggagaacc
    10261 tcctgatggc cgccacgctc gcctccggca cgacgacgct caaccacgcc gcatgcgaac
    10321 ccgaagtggc cgacctcgcg gagcttttga atcgcatggg cgcacgcgtg cgcggcgcgg
    10381 gcacgccttc gatcacgatt gaaggcgtcg agacgctcgg cgcggcggag cacacgatca
    10441 tccccgaccg catcgagacg ggcacgttcc tcgtggccgc cgccatcacg gacggcgagc
    10501 ttgagttgaa agattgcacg cccgaacatt ccgccaccgt catcgccaaa ctgcgcgagg
    10561 cgggcgtcga gattgaagag gtcaatccga gcactctgca tgtcaggcgt gcgcccggag
    10621 ggttgaaagc ctctgacatc acgaccgagc cgcacccgcg ttttcccacc gacatgcagg
    10681 cgcaatacat gactctgatg acgcaatcac aggggcgcgc ctcgatcacc gagacgatct
    10741 tcgagaaccg cttcatgcac gcctccgaga tgcagcgcat gggcgcgcgc atcgcgattg
    10801 aagggcgcac ggccatcgtc gaaggcgcaa cgccgctcgc gggcgcacgc gtgcaagcct
    10861 cggacttgcg cgcctcggcc tcgctcgtcc tcgccggact cgtcgccgaa ggcgagacga
    10921 tcatcgagcg cgtctatcac attgatcgcg gctacgaaaa gatcgaagac aaactgcgcg
    10981 gcgtcggcgc acggatcgaa cgcctccgcg actcggttac ggcgagcctc ccgcccatcg
    11041 ccgaagtcgg cgtgttatga aaactgtgag tagcccgtcg cctccggcta gcaggcaagt
    11101 tgccggagag ttgccgcaga atggatttgc acaactgatg acgaccgact gacgacggac
    11161 tttttaccta tcatctaact tatgtctcaa caaagctgca tcttttgcca ggtcgtcgcg
    11221 ggcgagattt ccgcagacat cgtctacagg gatgatcgcg ccgtggcgtt tcgcgacatc
    11281 aacccgcagg ctcctgtcca cttgctcgtc atcccgcacg agcacctcga ttcgctcgac
    11341 gacgccgcgg gacgcgacga agcgacgctc ggccatttgc tgcgcatcgc ggcgcgcgtc
    11401 gccaacgagc aagggttatc ggagtcgggc tatcgcaccg tgatcaatac gggcgcgggc
    11461 gcaggccagt cggtctttca cctgcacctg cacgtggtcg gcggtcgccc gctgcactgg
    11521 ccgcccggtt agaaaggaga cgagttatgt acgaagagtc ggaagacccg aaggctgtgg
    11581 tgagccgctg gtttgaagag gtgtggaaca aaggacggga agaggcgatt gacgagttgt
    11641 tcgcggagga cggcgtcgcg cacgggttgg ctgatgagac gggggagcca ttgcgcggcg
    11701 cgtcgggctt caaaccgttc ttccgcaatt ttcgcagcgc gttccctgac atcgaagtgg
    11761 ttgtcgaaga cgccatcgcg gagggcgaca aggtggcggc gcgttgccgc gtgcgcgcca
    11821 ctcacgcagg cgacagtctc ggattcgcgg cgacgcagag agcgatagag ttcacgggta
    11881 tgacttttct gcgagtgcgg gacggcaaga tcgtcgaagc gtggaacaac ttcgacttcc
    11941 tggggctgcg ccagcaactc ggcgcactct aaaaataaac gatgaaggag gaacgatgaa
    12001 ggatgaacta aaggcaactt gcttttagtt catccttcat cgttcctcct tcatcgtttc
    12061 tctctagctc gccgcgcgtt gcttgagtcg ctcctcttgc tgttgcccgg cctgttgcac
    12121 cttctcttcc ttgagcaggg aggtttcgta tttcacgttg ccgagcagcg tgtcggagat
    12181 ggctttgacg aagccgatga tggcgggacc gaggacgatg ccggggatgc cgaaggtata
    12241 gacgcctgcg atgagcgcga ggaacatcca gtagaagttg aaacgccccg cgccgctcgc
    12301 cgccacgcgc gggcgcagca agagcgacga gccgaccgtc atcataatgc cgatgacgtg
    12361 atagacggac gcgccgacgg ggtcgttgcg gaacacgagc aggtagacgc tgacgggcac
    12421 gtagatcgcc cactcgccga gcagcgggaa gaagcccgtg agaaacgtca cgattgagag
    12481 cacgagcgcg agcgggatgc cgaagac
//
